LOCUS       DASVOD010000001         6795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_887058, whole genome shotgun sequence.
ACCESSION   DASVOD010000001 DASVOD010000000
VERSION     DASVOD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887058"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF720_00005"
     CDS             complement(<1..925)
                     /locus_tag="VF720_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877757.1"
                     /translation="MNSSTCTPARRRNRHALAPATFRALLITLFMVAAPGLARLARSQ
                     AICPGDMGSAPDGIRAYSNVSGAPVTGHFPTRRNGPNGCALYTAVPDNERFFLGWGLD
                     YEIDAMGCASGSYYIDELDPPSQCEIGLTGPTLYTFDSGGNEVPLAGTGPNPYLGMPG
                     TLARWGQEPGDNLDLFVQNLGADTVYLNLLIDWDRDGTWGVNGQFSGPGELTPAGVGG
                     RAADMGSVAANSHLVLAQLWNRSVYNFPIPPSYIGFVSYLNPPSFPIGGNGGYHWGRF
                     TITRGQLPSWWNGSGNGQGGAVSDFIIRNPQG"
     gene            complement(1197..1451)
                     /locus_tag="VF720_00010"
     CDS             complement(1197..1451)
                     /locus_tag="VF720_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX7877758.1"
                     /translation="MMQEMLLGATAMAAMTVSLFFLRHWRDTRDRLFLFFAIAFALDG
                     AGRVFLGIGSARQESEPLSYLIRLLSFSLIIAAIVDKNRR"
     gene            complement(1456..1713)
                     /locus_tag="VF720_00015"
     CDS             complement(1456..1713)
                     /locus_tag="VF720_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HEX7877759.1"
                     /translation="MAGLIYSLCAATALLCAILLLQAYGRSHYKLLLWSGLCFVGLTL
                     NNLLLVVDKLLIPSHDLSSWRTATALISMSILLYGLIWDRE"
     gene            complement(1713..2102)
                     /locus_tag="VF720_00020"
     CDS             complement(1713..2102)
                     /locus_tag="VF720_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877760.1"
                     /translation="MTDDPISPDVRQFLLEHIDSVGELEALLILRREPEIQWNVTLIA
                     QRLYITEVAASQLLAHLVVQRLVRREGDDVPGYRYNPESEELERLVTATRETHARHLV
                     PVTNLIHSKSRTRIQEFADAFKLRREK"
     gene            complement(2203..3657)
                     /locus_tag="VF720_00025"
     CDS             complement(2203..3657)
                     /locus_tag="VF720_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7877761.1"
                     /translation="MNTPAFPDTNLLIAGERPAASNGAVQPTINPATAEKLIDVASAS
                     ADDARKAVDAAHHAFKSGVWSGLAAVDRGRVLFKLADLLEANADQVATLETLDNGKPF
                     FESRRIDVPLAVEAIRYYAGWANKVHGEVLPVRGQFHTYTRHEPLGVVAAITPWNFPL
                     LLSVYKVAAALAVGNTVVLKPAELTPLSCLRLGELALEAGVPPGVLNILTGKGSVAGQ
                     ALLEDPRVAKIAFTGSTPVGKSIMRGAADTLKRLSLELGGKSANIVFEDADLKGAVRG
                     AINGIFYGKGEICTAGSRLLLHESIHDEFVEMLKSTAEGMKQGDPFDSKTRIGAQVSE
                     AHMNSILAYIASGKDQGARVVTGGERNVIGNGFFVKPTIFDNVTPGMTIAQEEIFGPV
                     VAVMKFSNDDDAISLANDHMYGLAAGLWTNDLRRAHRLSARLEAGTVWVNTYNFYDPA
                     APFGGYKQSGFGRELGMHALAEYTQVKTVWIDLN"
     gene            3722..4213
                     /gene="purE"
                     /locus_tag="VF720_00030"
     CDS             3722..4213
                     /gene="purE"
                     /locus_tag="VF720_00030"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX7877762.1"
                     /translation="MPPLVGVIMGSKSDWETLRHASETLDSLGIVHEVRVVSAHRTPD
                     LLFEYAAGAEARGLEVLIAGAGGAAHLPGMAAAKTLLPVIGVPVQSAALNGLDSLLSI
                     VQMPGGVPVATMSIGRAGAINAALTAAAILGNGHPAIREALRRFRDAQTRAVLDDPDP
                     RTS"
     gene            4248..5372
                     /locus_tag="VF720_00035"
     CDS             4248..5372
                     /locus_tag="VF720_00035"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX7877763.1"
                     /translation="MRIGVLGGGQLGRMLALAGVPMGHSFTFLDPGDAAPMAPVATQI
                     RADYHDPAGLDRLARASDVVTWEFENVGAGPLSRLAEDGVPVRPGPGSLEFKQDRLDE
                     KLFFRRLGIPTTEFRAAATRDEFDDALAALGLPVVVKTRRQGYDGKGQSVVEDRAGAE
                     AAWQQLGGQPLLVECKVPFDRELSILAVRGADGACDFWPLVENVHAEGILRVSLAPIR
                     GGTPELETRADRLIRSILESLEHVGVLALELFQVGDDLLANEMAPRVHNSGHWTIEGA
                     VTSQFANHVRAISGARLGSCAPIGHAAMVNLVGRVPEPATLLALDGASLHLYGKTPAP
                     GRKLGHVTVMGRTPRERDERLAALLRSTDNTVLAPKPSII"
     gene            5567..6580
                     /locus_tag="VF720_00040"
     CDS             5567..6580
                     /locus_tag="VF720_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877764.1"
                     /translation="MIKRSPPRTAALRLATIMSIVGACVLAAPVARAAGTPACREIEN
                     QASASWTIGTTTFTGTSNKVTTTVAEILNVITTWQDATPVVVMSGATSQVLTFLVTNT
                     GNGTDSYTLTPTANLGGDSFDPSFVSLVLDANRNGTFDNGVDQPYVAGVNDPVLAADS
                     SLVVFALNDMPGGLNDGDTGDCRLTATSNTGSGTPGTVVPGAGECGNNAVVGASGGES
                     GDVGTYVKSGVVVSVVKAADIEDPFGGSEPVPGATITYTIQVAVTGSGIASGLDIVDP
                     IPASTTYLAGSLTLDAGGLSDALDLDPGDVGGTTAGAVTVLLGDVAAGGGVRTITFTV
                     TID"
     gene            6595..>6795
                     /locus_tag="VF720_00045"
     CDS             6595..>6795
                     /locus_tag="VF720_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877765.1"
                     /translation="MKMFLGLVFGLVLATGTAAAADTIRLRTEALTEKTVVDEKGKSE
                     TTLVTAARVAPGDEVVYAIHWEN"
BASE COUNT         1162 a         2241 c         2242 g         1150 t
ORIGIN      
        1 caccctgcgg attgcggatg atgaagtccg aaaccgcgcc gccctgcccg ttgccgctgc
       61 cgttccacca ggatgggagc tgcccgcggg tgatcgtgaa ccgcccccag tgatagccgc
      121 cgttgccgcc gatcgggaag ctgggcgggt tcaggtagga aacgaagccg atgtaggagg
      181 gcggaatcgg aaagttgtag accgaccggt tccagagctg ggcaaggacg aggtgtgaat
      241 tcgccgccac cgatcccatg tccgcggcgc ggccgcccac gcccgccggt gtcagttcgc
      301 ccggtcccga gaactgtccg ttcacgcccc acgtgccgtc ccggtcccag tcgatcagca
      361 ggttcaggta gaccgtgtcg gcgccgaggt tctgcacgaa gagatcgagg ttgtcacccg
      421 gttcctggcc ccacctggcc agcgtgccgg gcatgccgag ataggggttc gggccggtgc
      481 cggcgagcgg tacctcgttg ccgccactgt cgaacgtgta aagcgtgggc cctgtcaggc
      541 cgatctcgca ctggctcggc ggatcgagtt catcgatgta atacgaaccc gacgcgcaac
      601 ccatcgcatc gatctcgtaa tccaggcccc accccaggaa gaagcgttcg ttgtcgggta
      661 ccgcggtata gagcgcacag ccgttcggcc cgttgcgccg cgtcgggaaa tggcccgtta
      721 ccggagcgcc gctcacgttc gagtaggcgc ggatgccgtc cggcgcgctt cccatgtcgc
      781 cggggcagat ggcctgcgat cgcgcgagtc gcgccaggcc cggtgcggct accatgaaga
      841 gggtgatgag gagcgcgcgg aaggtggccg gagccagcgc gtggcgattc cggcggcggg
      901 cgggggtgca ggtactcgag ttcatgacag gcctccgttc gtgtcccagg ttcacgtgtc
      961 gccgcgtggt gaggaacacg acgggttaac agacggaacg cggagagcag gaggtgtgcc
     1021 ggaaaaatgt cgtatcggct tagagaacag ctaagatatt tatttcataa tattttaggg
     1081 tcgacagact ggtgtgtgga catcaggata ccccagtcgc cggggcgatt tttgttaact
     1141 tgttttaacg tgcttccgtg gctgattcga tgaaagctgc ctcgtgggaa cggactctag
     1201 cgacgattct tgtccacgat ggccgcgatg atcaggctga aggacaggag cctgatcaag
     1261 taagacagtg gttcggattc ctgacgtgcc gaaccgatcc ccaggaagac ccgcccggct
     1321 ccgtccagtg caaacgcgat ggcgaaaaac agaaagagcc ggtcccgcgt gtcccgccag
     1381 tgacgaagga agaacaggct cactgtcatg gcggccatgg ccgtggctcc cagcagcatt
     1441 tcctgcatca ttccgtcact cccgatccca gatgagtccg tacaggagaa tcgacatcga
     1501 gatgagagct gtcgcggtgc gccacgagga caggtcatga gatggaatga gcagcttgtc
     1561 cacaaccagc agcaggttgt tcagtgtcag cccaacgaaa caaaggccac tccagagaag
     1621 cagcttgtag tgacttcggc cgtaggcctg cagcagtaga attgcgcaaa ggagcgcggt
     1681 cgccgcgcag aggctataga tcaagccggc catcactttt cccttctcag cttgaacgca
     1741 tcggcaaatt cctgaatccg ggtgcgtgac ttcgaatgga tcagattggt caccggaacc
     1801 aggtgccttg cgtgcgtctc ccgagttgcg gttacgagcc gttccaactc ctccgactcg
     1861 ggattgtatc gatagcctgg aacgtcgtcc ccctcacgac gcaccagacg ctgaacgacc
     1921 agatgcgcca ggagttggct ggccgcaacc tccgtgatgt acaaccgctg ggcgatgaga
     1981 gtgacgttcc actggatctc cggctccctg cgaaggataa ggagggcttc gagttcgccc
     2041 accgagtcga tgtgctccag cagaaactgc cggacatccg gagagatggg atcatcggtc
     2101 atcttgtgag tttgtctgtg gaaagtatcc ctgggtcatg acctcctccg cgggaagcaa
     2161 tcgtcagaaa ctggcctcgg agggtgtcgc gggacacgtc cctcagttca aatcaatcca
     2221 caccgtcttc acctgcgtgt actcggccag cgcgtgcatc cccagctcac ggccgaagcc
     2281 gctctgcttg tagccgccga acggggccgc cggatcatag aagttatagg tgttcaccca
     2341 caccgtgccc gcctccaacc gagccgaaag ccggtgcgcc cggcgcaggt cgttggtcca
     2401 gaggccggcc gccaggccgt acatgtgatc gttggccagg gagatggcgt cgtcgtcatt
     2461 cgagaatttc atgacggcca cgaccggtcc gaagatctcc tcctgggcga tggtcatgcc
     2521 gggcgtcacg ttgtcgaaga tggtcggctt cacgaagaag ccgttgccga tcacgttccg
     2581 ctcgccgccg gtcacgacgc gcgccccctg gtccttgccc gaggcgatgt aggccaggat
     2641 ggaattcatg tgggcctcgc tcacctgggc gccgatccgc gtcttggaat cgaacggatc
     2701 cccctgcttc atcccctcgg cggtcgactt gagcatctcc acgaactcat cgtggatcga
     2761 ctcgtggaga aggagccgcg agccggcggt gcagatctcc cccttgccgt agaagatgcc
     2821 gttgatggcg ccgcgcacgg cccccttcag gtcggcgtcc tcgaagacga tgttggcgct
     2881 cttgccgcca agctcgagcg agaggcgctt caacgtgtcg gccgcgccgc gcatgatcga
     2941 cttgcccacc ggcgtcgaac cggtgaaggc gatcttggcc acgcgcggat cctcgagcag
     3001 cgcctgtccg gccaccgatc ccttgccggt cagaatgttg agcacgccgg gcggcacgcc
     3061 cgcttccagc gccagctcgc cgaggcggag gcacgataag ggcgtcaact cggcgggctt
     3121 gagcaccacc gtgttcccga cggccagcgc cgccgccacc ttgtacaccg acaggagcag
     3181 cgggaaattc cacggcgtga tggcggccac cacgcccagc ggctcgtgcc gcgtgtaggt
     3241 gtggaactgc ccgcgcaccg gcagcacctc gccgtgcacc ttgttggccc agccggcgta
     3301 gtagcggatc gcttccaccg ccagcggcac gtcgatgcgg cggctttcga agaagggctt
     3361 gccgttgtcg agcgtttcga gcgtggccac ctggtcggcg ttggcctcga ggagatcggc
     3421 cagcttgaag aggacgcgac cccggtccac cgcggccagg ccggaccata ccccgctctt
     3481 gaacgcgtgg tgcgccgcgt ccacggcctt ccgcgcgtcg tccgccgagg ccgaggccac
     3541 gtcaatgagc ttttcggccg tggccgggtt gatcgtgggc tggacggcgc cgttcgaggc
     3601 cgccgggcgc tcgccggcga tgaggaggtt ggtatcgggg aaggcgggtg tattcatggt
     3661 cggacccttt ccgggagtcg ggtgggtgtc agggggaggg agtataaagt aacctagccc
     3721 catgccaccc ttggtaggcg tcatcatggg ctcgaaaagc gactgggaga cgctgcgtca
     3781 cgcctccgaa acgctcgact cgctggggat cgtccacgaa gtacgggtgg tttcggccca
     3841 tcgcacgccc gacctcctct tcgagtacgc ggcgggagct gaggcgcggg gcctcgaggt
     3901 gctgattgcc ggcgcggggg gcgcggccca cctgccggga atggcggccg ccaagacgct
     3961 cctgccggtc atcggcgtgc cggtccagtc cgcggcgctg aacgggctcg actccctcct
     4021 atccatcgtg cagatgccgg gcggggtgcc cgtggccacc atgtcgatcg gccgggcggg
     4081 ggccatcaac gcggccctga ccgcggccgc catcctggga aacgggcacc cggccatccg
     4141 cgaggccctt cgtcggttcc gcgacgccca gacgcgcgcg gtgctcgacg accccgaccc
     4201 tcgcacgtca tgaacgccgc ggtccgttcc gccgcccgga acaggtgatg cgcatcggcg
     4261 tcctgggcgg cggccagctc ggccggatgc tggccctggc cggcgtgccg atggggcatt
     4321 cgttcacctt cctcgatccg ggcgatgcgg cgccgatggc gccggtggcc acccagatcc
     4381 gggccgacta ccacgatccg gccggcctcg accgactcgc ccgggccagc gacgtggtga
     4441 cgtgggaatt cgaaaacgtt ggcgcggggc cgctttcgcg gctggccgag gatggcgtgc
     4501 cggtccggcc aggccccggc tcgctcgagt tcaagcagga ccgcctcgac gagaaactct
     4561 tcttccggcg gctgggcatc cccaccacgg agttccgtgc ggccgccacc cgggatgaat
     4621 tcgacgatgc gctcgcggcg ctgggcctcc cggtcgtcgt caagactcgt cgccagggtt
     4681 acgacggcaa gggacaatcg gtggtcgaag accgggccgg cgcggaggcg gcctggcaac
     4741 agctcggcgg gcagccgctc ctcgtggagt gcaaggtgcc gttcgaccgc gagctctcca
     4801 tcctggcggt ccgcggagcg gacggcgcct gtgatttctg gccgctggtg gagaacgtgc
     4861 acgccgaagg aatcctgcgg gtgagcctgg cgcccatccg cggcggaacg cccgaactcg
     4921 agacgcgggc cgatcgattg atccggtcga tcctcgaatc cctggaacat gtgggcgtcc
     4981 tggcgctcga gttgttccag gtcggcgacg atctccttgc caacgagatg gccccgcgcg
     5041 ttcacaattc cggccactgg acgatcgaag gggccgtgac gagccagttc gccaaccacg
     5101 tgcgcgccat tagcggggcg cggctcggtt cgtgcgcgcc catcggccac gccgcgatgg
     5161 tgaacctcgt gggccgggtg cccgagccgg ccaccctcct cgcgctggat ggcgcgtctc
     5221 tccacctcta tggaaagacg ccggcgcccg gccggaagct gggtcacgtg accgtcatgg
     5281 ggcgcacgcc gcgtgagcgg gatgaacgtc tcgcggcgct ccttcggtcg acggacaaca
     5341 cggtcctggc cccgaagcca tccatcatct gatcgcaatg ccatcgatat gactgttacc
     5401 actcgatgag gggagtccgt gccttcgcgt cttattgcat gttctcacta ttaacaagat
     5461 aagaccttta attaactcaa gttagcgagg acaaggccga ttccgtggcc acgacccgac
     5521 gggcgctttc gcccgaggcg ggcaattcac cggccacgga gatcccatga tcaagaggtc
     5581 gccacctcga accgccgcgc ttcgccttgc cacgatcatg agcatcgtcg gggcgtgcgt
     5641 cctggcggct cctgtcgccc gcgccgcggg caccccggcc tgccgcgaaa tcgagaacca
     5701 ggcgtccgcc agctggacca tcggcaccac caccttcacc ggcaccagca acaaggtgac
     5761 gacgacggtg gccgagatcc tgaacgtcat caccacctgg caggacgcca cgccggtggt
     5821 ggtgatgtcg ggcgccacgt cgcaggtgct gaccttcctc gtcaccaaca ccggcaacgg
     5881 caccgattcg tacacgctga cgccgaccgc caacctcggc ggcgactcct tcgatccgtc
     5941 gttcgtgagc ctcgtgctcg acgcgaaccg gaacggcacg ttcgacaacg gcgtcgacca
     6001 gccctatgtg gccggcgtca acgatcccgt gctggccgcc gattcgtcgc tggtggtctt
     6061 cgccctgaac gacatgccgg ggggcctcaa cgacggcgat accggcgact gccggctgac
     6121 cgccacctcg aacaccggct ccggcacacc gggcaccgtc gttccgggcg ccggggagtg
     6181 cggcaacaac gccgtggtcg gcgcgtcggg tggcgagtcg ggcgacgtgg gcacctacgt
     6241 caagtcgggc gtcgtcgtct cggtggtcaa ggccgccgac atcgaggacc ccttcggcgg
     6301 ctccgagccc gtaccgggcg ccacgatcac ctacaccatc caggttgccg tcacgggaag
     6361 cggtatcgcc agcgggctcg acatcgtcga cccgattccc gccagcacga cttacctcgc
     6421 cggctcgctc acgctcgacg cgggcggcct ctccgacgcg ctcgatctcg atccgggaga
     6481 tgtcggggga accaccgcgg gcgccgtcac cgtgcttctt ggtgacgtgg ccgccggcgg
     6541 cggcgttcgc acgatcacgt tcaccgtcac catcgattga gggggatacc gatcatgaag
     6601 atgttcctgg gcctggtatt cggcctcgtg ctcgcgacgg gcaccgccgc cgccgccgac
     6661 acgatccggc tccgcaccga ggcgctgacc gagaagacgg tcgtcgacga gaaggggaag
     6721 tcggaaacca cgctcgtcac cgccgcccgc gtggcgcccg gcgacgaggt cgtctacgcc
     6781 atccactggg agaac
//
LOCUS       DASVOD010000002         3850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_887244, whole genome shotgun sequence.
ACCESSION   DASVOD010000002 DASVOD010000000
VERSION     DASVOD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887244"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VF720_00050"
     CDS             <1..904
                     /locus_tag="VF720_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7877766.1"
                     /translation="DFWDYFPTPGGGYPASLPAPLGHGAVPANVMRHYRNVIWVGNNF
                     NGDQNGWYDSPILSYLEAGGNVMLLSRQGEVFLTPALQDYLGINWSATGTTITDCIAF
                     HPGLTTIARTGTQNLVSVFNQTLTRADADLLYRAEAGFTPDVGIGVLRRPSAGGTYRT
                     NGGRFIFLSGRPYRWNSTNLKGNIMYLLLNKFFEPLDQSAVPEAPGVPVGARLLSVRP
                     NPTSSASRVSFELPHAGVVRLSIVDVSGRLVRRLVDGEMALGAHEVSWDGCDEGGRDA
                     ASGMYYAILEADGEKSTRTLVRVR"
     gene            complement(917..1888)
                     /gene="nadA"
                     /locus_tag="VF720_00055"
     CDS             complement(917..1888)
                     /gene="nadA"
                     /locus_tag="VF720_00055"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX7877767.1"
                     /translation="MTVSALLDLEAEIRRLKTERKAVLLAHYYQESEIQDVADFVGDS
                     LQLSQEAAKTDADVILFAGVHFMAETAKIVNPTKTVILPDLEAGCSLAEGCPAPQFEA
                     FLQRYPGHTVISYINCSAAVKALSDIICTSSNAVAVVNSVPKDTPIVFAPDQHLGRWV
                     IKQTGRPMVLWPGSCLVHELFNEREIVHLKHENPGAPVLAHPECDERVLAHADYIGST
                     SGILGEVIRNPATTFIIATEPGIIHQMEKSAPHKRFIPAPAGNGCACNTCPFMRKNTL
                     EKVYAALHDLKPEITLDEDLRARAEKPLRRMLEISAAPKANPTPYKD"
     gene            1925..2521
                     /gene="thpR"
                     /locus_tag="VF720_00060"
     CDS             1925..2521
                     /gene="thpR"
                     /locus_tag="VF720_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX7877768.1"
                     /translation="MDTGHPPTEARTRAFVAVHPDAPTLAAIADLLAMLQAREREAIR
                     WIRPEQAHFTLRFFGDCDAAEVEAAARVLSAVAAAAAGFRLSLDGLGAFPDWRRPRIV
                     WLGSGEGGGTLEQLARTLDAAFRDVGLDRSDRPFRAHLTLGRVREGCTLSPATLALLE
                     GMRPVLPAFQVESVQLVASRLTPSGAIHRAIASARLEG"
     gene            2752..>3850
                     /locus_tag="VF720_00065"
     CDS             2752..>3850
                     /locus_tag="VF720_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877769.1"
                     /translation="MPEHPVRPAPPRKAHILAARRAAAFVAFLLTAGITGSASARPRP
                     EEDDLSTGPLHPMVYAINDPYIFTHNAAQLTLQITNVGLLGNPFIDEVSAGWRGGEYL
                     FQAGLWIGALGEDGEAHVSQSIGRNNVEFRPSIQAVDTIYESYEGIRNGNRRTEAFDA
                     DDDKDGLDDEDFQNGKDDDLDGQIDEDFAAIGQQMFSCEYRDDTQEAVNQLGQHRPLG
                     LRVRQRSFQWSTAGINEFTGIDFDVQNVGDQRLSDVYIGFYVDADAGPKSAPNFFSDD
                     LVGFTQLDTIPPVEGNAGMCERERLSLDIAWIHDAPDNGTTVVGGDVTGWFGGLFLGH
                     TTDPAGVRAPERVGLTTVRWTSSSAPYPEGDP"
BASE COUNT          566 a         1256 c         1325 g          703 t
ORIGIN      
        1 tgatttctgg gattacttcc cgacgccggg gggcggctat cccgcatcgc tgccggcccc
       61 gctggggcat ggcgccgtgc ccgcgaacgt gatgcggcac tatcgcaacg tcatctgggt
      121 ggggaacaac ttcaacggcg accagaacgg ctggtacgac tcgccgatcc tgtcgtacct
      181 cgaggcgggc ggcaacgtca tgctcctgtc gcggcaggga gaggtgttcc tgacgccggc
      241 gttgcaggat tacctcggca tcaactggag tgccacgggt acgaccatca cggactgcat
      301 cgcgttccat ccgggtctga cgacaattgc ccggaccggc acgcagaacc tggtttcggt
      361 tttcaaccag acgctgaccc gtgccgacgc cgatctgttg taccgggccg aagcgggctt
      421 cacgccggat gtcggcatcg gcgtcctgcg tcgcccgtcg gccggcggca cctaccggac
      481 gaacggcggt cgcttcatct tcctgtcggg ccgtccgtac cgctggaaca gcaccaatct
      541 caagggcaac atcatgtacc ttctgctcaa caagttcttc gagcccctcg accagagcgc
      601 ggttccggag gcgccgggcg tgccggtcgg ggcgaggctg ctgtccgttc gtccgaaccc
      661 gacatcgagc gcttcgcgcg tgtcattcga gttgccgcat gccggcgtgg tgcgactgtc
      721 gatcgtggat gtatccggtc ggctggtgcg gcgtctcgtg gatggggaga tggcgctcgg
      781 tgcccacgag gtttcgtggg atgggtgcga tgagggtggt cgcgacgcgg cttccgggat
      841 gtactacgcg attctcgagg cggatgggga gaagtcgacg cgaacgctgg tgcgggtgcg
      901 ctaggacgac ccggcgtcag tccttgtacg gcgtggggtt ggccttcggc gccgcgctga
      961 tttcgagcat ccgccgcagc ggcttctccg cgcgcgcgcg aagatcctcg tccagcgtga
     1021 tctcgggctt gaggtcgtgc agcgccgcgt acaccttctc cagcgtgttc ttccgcatga
     1081 aggggcaggt gttgcaggcg cagccgttgc cggcgggggc agggatgaag cgcttgtggg
     1141 gcgccgactt ttccatctgg tggatgatgc ccggctcggt ggcgatgatg aacgtggtgg
     1201 ccgggttccg gatcacttcg cccaggatgc ccgacgtcga gccgatgtag tcggcgtggg
     1261 cgagcacgcg ctcgtcgcac tcggggtggg cgagcacggg cgcgccgggg ttttcgtgct
     1321 tcaggtgcac gatctcccgt tcgttgaaga gttcgtgcac gaggcaggag ccgggccaga
     1381 gcaccatcgg ccggccggtc tgcttgatga cccagcgccc gaggtgctgg tcgggcgcga
     1441 agacgatggg tgtgtccttc gggacggaat tcaccacggc caccgcgttg ctcgacgtgc
     1501 agatgatgtc cgagagcgcc ttcacggccg ccgaacaatt gatgtagctg atgacggtgt
     1561 ggccggggta gcgttggagg aaggcctcga actggggcgc gggacaccct tcggccagcg
     1621 agcagccggc ctcgaggtcg gggaggatca cggtcttcgt cgggttgacg atcttggccg
     1681 tctcggccat gaagtggacg ccggcgaaga gaatcacgtc ggcgtcggtc ttcgccgctt
     1741 cctggctgag ctggaggctg tcgccgacga agtcggccac gtcctggatt tcggattcct
     1801 ggtaatagtg ggccagtaac acggccttgc gttccgtctt gagccggcgg atttcggctt
     1861 cgagatcgag gagggcggag acggtcatgc ggaaagctta tcaccgcccg gagtcccgcg
     1921 cctgatggac acggggcatc cgcccaccga agcccgcacc cgggccttcg tggccgtcca
     1981 tccggatgcc ccgaccctgg ctgccattgc ggacctcctg gccatgctcc aggctcgcga
     2041 gcgagaggcc atccgctgga tccggccgga acaggctcat ttcacgcttc gttttttcgg
     2101 cgattgcgat gcggcggagg tcgaggcagc cgcgcgcgtc ctgtcggccg tcgctgcggc
     2161 cgcggccggg ttccgccttt cgctggacgg cctcggcgcc tttcccgact ggcgccgtcc
     2221 ccggatcgtc tggttgggtt cgggggaggg gggcggcacg cttgaacagc tggcgcgcac
     2281 gctcgacgcg gccttccgcg acgtcgggct cgatcgttcc gatcgcccct ttcgcgccca
     2341 cctcacgctc ggtcgcgtgc gggaggggtg tacgctgtcg ccggcgacgc tggcgctcct
     2401 cgagggcatg cgtcccgtgc ttcccgcatt ccaggttgag tccgtccagc tcgtggccag
     2461 ccggctgacg ccgtcggggg ccattcatcg cgcgatcgct tcggcgcggc tcgagggatg
     2521 attttccggt cggccgcctt gcgccgatcg gggacactac ctatagtcga ttccagaggc
     2581 cggaacacca atgctcgtcc tcccctcccg gcgctgggtg acccaccctt cggtcactcc
     2641 cgtttcagac ggttgttcat cgccccctcg ggtcacgcgg atcggccata gccgctccct
     2701 cgtacgtacc ggcagctgcc ggtgcgtcgg cgttcgtcac cggaggtccc catgcccgag
     2761 cacccggttc ggcccgcccc ccctcggaag gcgcatatcc tggcggcgag gcgtgccgcc
     2821 gcgttcgtcg ctttcctgct gacagccgga atcacgggga gtgcttccgc acgtcctcgt
     2881 ccggaagagg acgatctttc cacggggccg ctccacccca tggtctacgc catcaacgac
     2941 ccctacatct tcacccacaa cgcggcccag ctcacgctcc agatcacgaa tgtcggcctc
     3001 ctgggcaacc cgttcatcga cgaggtgtcg gcggggtggc gcggcggcga gtacctcttc
     3061 caggcggggc tctggatcgg cgcgctcggg gaggatggcg aggcgcacgt ctcccaatcg
     3121 atcgggcgca ataacgtgga gttccgcccg tcgatccagg cggtcgacac catctacgag
     3181 tcgtacgagg ggatccgcaa cggcaatcgc cgcaccgagg ccttcgatgc cgacgacgac
     3241 aaggatgggc tggacgacga ggatttccag aacggcaagg atgatgacct cgacggccag
     3301 atcgacgagg actttgccgc catcggccag cagatgtttt cctgcgagta ccgggacgac
     3361 acgcaggagg cggtgaacca gctcgggcag caccgcccgc tcgggttgcg tgtgcgccag
     3421 cgttcgttcc agtggtccac cgcggggatc aacgagttca ccggcatcga cttcgacgtg
     3481 cagaacgtgg gcgaccagcg cctgagcgac gtgtacatcg gtttctacgt cgatgccgac
     3541 gccggcccga agagcgcccc caatttcttt tccgacgacc tggtcggctt cacccagctc
     3601 gacacgatcc cgcccgtgga ggggaacgcg gggatgtgcg agcgggaacg cctcagcctc
     3661 gacatcgcct ggatccacga cgcgcccgac aacggcacga cggtcgtggg cggcgacgtg
     3721 accggctggt tcggtgggct ctttctcggg cataccaccg accccgccgg cgtgcgggcc
     3781 ccggaacgcg tgggcctgac caccgtgcgc tggacatcgt cttcggcgcc ctatccggaa
     3841 ggggatccgg
//
LOCUS       DASVOD010000003         3150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_156905, whole genome shotgun sequence.
ACCESSION   DASVOD010000003 DASVOD010000000
VERSION     DASVOD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156905"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="VF720_00070"
     CDS             complement(<1..370)
                     /locus_tag="VF720_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX7877770.1"
                     /translation="MTRNQKPHGARRAALALTVGVSAIAIATQAADGPGVALTVYNGN
                     LGLVKDTRSVDLKSGNTTIRFDDVAALIDPTSVHVKALQHPADVAVLEQNYQYDLADA
                     ERILQRYLNQSVTAIMKDGNS"
     gene            complement(367..3036)
                     /locus_tag="VF720_00075"
     CDS             complement(367..3036)
                     /locus_tag="VF720_00075"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX7877771.1"
                     /translation="MATRELAKSFDPAQVEERLYRAWMDSGQFHARVDGARKPFTIMI
                     PPPNVTGILHMGHVLDNTIQDVVIRFRRMQGWEALWQPGTDHAGIATQNVVEKKLRDE
                     GTSRKEIGREAFLERVWKWREEYGGTIIRQLQRLGASCDWERERFTMDEGLSKAVEEV
                     FLRLYEKGLIYRGYYIVNWCPRCQTAISDEEVEMVETEGRLHFVTYPFDIGGTDGITV
                     ATTRPETILGDVAVAVNPEDERYRHLVGRMVHVPFVNRPIPIIADDYVEKDFGAGALK
                     VTPAHDINDFELGKRHGLEPIVVIDEAGTMNGAAGPFAGQDRLEARKHVVRDLKEMGL
                     LVKVENHHMKLGHCQRCDTVIEPLLSRQWFVKMAPLAAPAVEALEEGRLAFVPEHWNK
                     VYLNWMTNIRDWCISRQLWWGHRIPVWYCDNDQTPHAGHNAPGACTTCGHRSFTQDPD
                     VLDTWFSSWLWPFSTLGWPEKTPDLDYFYPTQFLTTGPDIIFFWVARMVMAGREFMGD
                     VPFPVVYLHGIVRDEQGRKMSKSLGNSPDPLDLIDRYGADALRYTMILLTPTGQDVLF
                     SEKKCEVGRNFANKIWNASRLALSHMEGFDPASVDIRSLPLEDVDRWILSRLDETVES
                     VTSNLETFRYNEAARDLYEFAWGDFCDWYLEIAKRRFYAEPGPERTTSQAVTWGVLAT
                     LLHLLHPFLPFLTEELWSVLPGGSSDGAGMLIRRPWPACDAARRDPAAAAGLAHLKEV
                     VTSIRNLRTEMNVPPGREVAVVLRARPNESGILSRLSGYVRSLAKVGDLVIAPDAPRP
                     EVAAASVVGETEIFLSLAGAIDLAAETARLTKELDKIEKQAQSVEKKLANEDFRAKAR
                     PEVVDAEVERLKLLRDTRARLADNLAILSGGKG"
BASE COUNT          501 a         1019 c         1076 g          554 t
ORIGIN      
        1 cggaattgcc gtctttcatg atcgcggtga ccgactggtt caggtagcgc tggaggattc
       61 gctcggcgtc ggccaggtcg tactggtagt tctgttcgag gacggccacg tcggcgggat
      121 gctggagtgc cttcacatgc acgctggtcg ggtcgatcag ggccgccacg tcatcgaagc
      181 ggatggtggt gttgcccgac ttgaggtcga ccgagcgggt gtctttcacg aggccgaggt
      241 tgccgttgta caccgtgagg gccacgccgg gaccgtcggc ggcctgcgtg gcgatggcga
      301 tggcgctgac ccccaccgtg agtgccaggg ccgcccggcg ggcgccgtgg ggcttctgat
      361 tccttgtcat cccttccctc ccgagagaat ggccaggtta tccgcgagtc gggcccgggt
      421 atcgcgcagg agcttgagac gctccacttc ggcatccacc acttcggggc gggccttggc
      481 gcgaaaatcc tcgttggcca gcttcttctc gacggactgg gcctgcttct cgatcttgtc
      541 cagttccttg gtaaggcggg ccgtttcggc cgccaggtcg atggctcccg cgagggagag
      601 gaagatctcg gtttcgccga ccaccgaggc ggcggccact tcgggccggg gcgcgtcggg
      661 cgcgatgaca aggtccccca ctttcgcgag agagcgcacg tacccggaaa gccgcgaaag
      721 gattcccgac tcattcggcc gggccctgag aaccaccgcc acctcccggc cgggaggcac
      781 gttcatctcg gtgcgcaggt tgcggatgga ggtgacgacc tccttcaggt gcgccaagcc
      841 ggccgccgcg gccggatccc gccgggccgc atcgcacgcc ggccaggggc gacggatgag
      901 catgccggcg ccatcggacg agccgccggg gagcaccgac cagagctcct cggtgaggaa
      961 cggcaggaag ggatggagca ggtggaggag agtggcaagc accccccagg tgaccgcctg
     1021 cgaggtggtc cgctccgggc ccggctcggc gtagaagcgc cgcttggcga tctccaggta
     1081 ccagtcgcag aaatcgcccc aggcgaactc gtacaggtcc cgggcggctt cgttgtagcg
     1141 gaacgtttcg aggttcgacg tgaccgactc caccgtctcg tcgagccggg agaggatcca
     1201 gcgatccaca tcctcgagcg gcaatgaccg gatgtcgacc gacgccggat cgaacccctc
     1261 catgtgggaa agggcgaggc gcgaggcgtt ccagatcttg ttggcgaaat tccggccgac
     1321 ctcgcacttc ttttccgaga agaggacgtc ctgtccggtg ggcgtgagga ggatcatggt
     1381 gtagcggagc gcgtcggccc cgtagcgatc gatcaggtcg agcggatcgg gcgaattgcc
     1441 gaggctcttg gacatctttc gcccctgctc gtcgcgcacg atgccgtgca ggtagacgac
     1501 cgggaacggc acatcgccca tgaattcccg accggccatg accatgcgcg ccacccagaa
     1561 gaagatgatg tcgggcccgg tggtcaggaa ctgggttgga tagaagtaat cgagatccgg
     1621 cgtcttttcc ggccacccca gcgtggagaa gggccacagc cacgacgaga accaggtgtc
     1681 caacacatcg gggtcctgcg tgaacgagcg gtggccgcac gtcgtgcagg cgccgggcgc
     1741 gttgtggccg gcgtgcggcg tctggtcatt gtcgcagtac cacaccggga tgcgatgccc
     1801 ccaccacagc tggcggctga tgcaccagtc gcggatgttg gtcatccagt tgagatacac
     1861 cttgttccag tgttcgggca cgaaggcgag ccgcccttcc tccagtgcct cgacggccgg
     1921 cgcggccaac ggcgccatct tcacgaacca ctggcgggag aggagcggct cgatgaccgt
     1981 gtcgcagcgc tggcagtggc cgagcttcat gtggtggttt tccaccttga cgaggagccc
     2041 catctccttg agatcgcgca cgacgtgctt gcgtgcctcc agccgatcct ggccggcgaa
     2101 cggcccggcg gccccgttca tggtgcccgc ctcgtcgatg acgacgatcg gctcgaggcc
     2161 gtgcctcttc cccagctcga agtcgttgat gtcgtgggcc ggcgtgacct tgagcgcgcc
     2221 cgcgccgaag tccttctcca cgtagtcgtc ggcgatgatc gggatcggac ggttcacgaa
     2281 cggcacatgc accatcctcc ccaccagatg gcggtagcgc tcgtcctccg ggttcacggc
     2341 cacggccacg tcgcccagga tcgtttcggg acgcgtggtg gccacggtga tcccatccgt
     2401 gccgccgatg tcgaaggggt acgtgacgaa gtgaagacgc ccctccgtct cgaccatctc
     2461 cacttcctcg tcggagatgg cggtctggca gcgcggacac cagttgacga tgtagtagcc
     2521 gcggtagatg agccccttct cgtacaggcg gaggaacact tcctccaccg ccttcgagag
     2581 cccctcgtcc atcgtgaacc gctcgcgctc ccagtcgcac gaggcgccga ggcgctggag
     2641 ctggcggatg atcgtgccgc cgtactcttc gcgccatttc cagacccgct cgagaaaggc
     2701 ctcgcggccg atctccttcc ggctggtgcc ctcgtcgcgc agcttcttct ccaccacgtt
     2761 ctgcgtggcg atcccggcgt ggtcggtgcc gggctgccac agcgcctccc acccctgcat
     2821 gcggcggaag cggatgacga cgtcctggat ggtgttatcg aggacatgcc ccatatgcag
     2881 gatgccggtc acgttgggcg ggggaatcat gatcgtgaac ggcttccggg ccccgtcgac
     2941 ccgggcgtgg aattgcccgg agtccatcca ggcccggtag aggcgttcct ccacctgggc
     3001 gggatcgaaa ctcttggcta gttctcgagt ggccatgtga tcgtctccgg cgcggccccg
     3061 ggcgcggaat tccggcccgg cgcaagggcg cccggtgaaa ggacggccgg aggatccagc
     3121 gatcgcggcc ggtgcggaac tggtttcggg
//
LOCUS       DASVOD010000004         2967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_417893, whole genome shotgun sequence.
ACCESSION   DASVOD010000004 DASVOD010000000
VERSION     DASVOD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417893"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VF720_00080"
     CDS             <1..1585
                     /locus_tag="VF720_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877772.1"
                     /translation="FASTRFVQRAEQEGLLATNLFSVFPDGTGLIRLTTDRNGAEEPS
                     VDPRNGRIVYARWWFNRHRPPVVPADTLTTDPDRAMPSDPVDLWHAISIRADGDGMRL
                     AGGWPRVRSQTMAYQPVLLHDGTLVGVTSATGSLLARPGERLEATLLVAYPGGFAPPV
                     PIAAGGVSPLALPDDRIVYSAPGPGGDLALFLVRSPGQAPVVLADTPGFDDLDAVVLM
                     PREAPARLDTYLPPPIREAPVSSRTPLEDPDWTFRFDCLNVFANGPVDSPFPDAPPIT
                     EGLRIRFFAAIERPGSAADSIVLVREAPVLDGGAVHEHEMPADTPMFEQLVDARGEVI
                     RGFNGPAHVPGFNFGRFGSGTKCVGCHAGHSAMEVARNNSLAAWTNLAPSARLTTQGV
                     TNPGQGTRTLVDRRAKGPIGQVGCRLRGDGEDRVRLSWPFPVEVKEVILYAGPRDRAH
                     GDDSRIRLTDITFLDADRPVHAGTITRDLDPAGTHLEVDNVRIDALDIHFRRVEGTID
                     HEPGGALSEIEVIGRLVDR"
     gene            1603..>2967
                     /locus_tag="VF720_00085"
     CDS             1603..>2967
                     /locus_tag="VF720_00085"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7877773.1"
                     /translation="MTRWFRKMSRLIPFVAAVALLAAGSSPALAQNGFYAVFSRDGTD
                     VWAVGGSGTLYRSFDGGLTWTTRTVGTDTHRGVTANGWTDILVSDNGRIHRSTDSGGT
                     WNTTTVAGSPSLRGVQLVDAMNGWIVGASGTILHTVDGGANWTPQASGTAVKLNALKF
                     LDATTGWVVGDGGTILKTTDGGASWTPLTSGTTKDLYGVDATTIGAVDPVVWAVGKDA
                     TALKSVNGGTNFTPIDLKIDSKADANGVYLASASAVTLVGGGGFIRVSADGGASWNFR
                     QHNIHGEMDGVYMAAGKGWAVNRSNRIVLRSGDAGVTFAPPAGALASRTLTLKQSDAG
                     AIIRGSTLAQNGQNKNSFYCVLSATVYKSLNRGETWAAIGTINTGSPYGAPSKTNAFY
                     VHPSDSTLMIAAVGATDRIVRSTNGGVTWTTVPGSVIPFTEYGVPLEMDVNDPNHLIL
                     GAEDQ"
BASE COUNT          474 a         1065 c          964 g          464 t
ORIGIN      
        1 cttcgccagc acgcgcttcg tacagcgcgc cgagcaggag gggctcctgg ccaccaatct
       61 cttctcggtg ttccccgacg gcacgggcct gatacgcctc accaccgacc ggaacggggc
      121 cgaagagccg tcggtcgatc cccgcaacgg ccggatcgtc tacgcgcgct ggtggttcaa
      181 tcgccatcgc cccccggtcg tgccggccga cacgcttacc accgatcctg atcgcgcgat
      241 gccgtccgat ccggtcgacc tatggcacgc catctccatc cgcgccgatg gtgacggcat
      301 gcgcctggcc ggtggatggc cgcgcgtacg gagccaaacg atggcctacc aacccgtact
      361 cctccatgac gggacgctcg tcggcgtgac gtcggccacg ggttcgctcc tggcccgccc
      421 tggcgagcgc ctcgaggcaa cgctcctcgt ggcgtacccg gggggttttg cgccgccggt
      481 tccgatcgcg gcgggaggcg tttcacccct ggcgctcccc gacgaccgca tcgtctattc
      541 ggcgcccggc ccgggaggcg acctcgcact cttcctcgtg cgttcacccg gccaggcgcc
      601 ggtggtgctc gccgacacac ccggcttcga cgacctcgat gcggtcgtcc tgatgccgcg
      661 cgaggcacct gcgaggctcg atacctacct tccgccgccg atccgcgagg cgccggtctc
      721 ttcgaggacg ccgctcgagg atcccgactg gactttccgc ttcgactgcc tgaacgtctt
      781 cgccaatggc ccggtcgatt cgccgttccc ggacgcgcct ccgatcacgg aggggctacg
      841 tatccgattc ttcgcggcga tcgagcggcc ggggagcgcg gcggattcga tcgtgctggt
      901 ccgcgaagcg ccggtgctcg atggcggcgc cgtccatgag cacgagatgc cggccgacac
      961 accgatgttc gagcaactgg tggatgccag gggcgaggtg atccggggat tcaacgggcc
     1021 ggcgcacgtg cccggtttca acttcggccg cttcggctcg ggcaccaagt gcgtcgggtg
     1081 ccacgccgga cactcggcga tggaagtggc ccggaacaat tcgctggcgg cgtggaccaa
     1141 cctggctccc tcggcccggc tcaccaccca gggggtgacc aacccggggc aggggacgag
     1201 gaccctggtc gatcggcggg ccaaagggcc catcggccag gtagggtgcc ggctccgggg
     1261 ggatggggag gaccgggtcc gcctctcgtg gccgtttccc gttgaagtaa aggaagtcat
     1321 cctctacgct ggaccgcggg accgggcgca tggagatgac tcccgcatcc gattgaccga
     1381 catcacgttt ctggacgccg accgaccggt tcatgcgggc accatcaccc gcgatctcga
     1441 cccggccggc acccaccttg aagtggacaa cgtccggatc gacgccctcg acatccactt
     1501 tcgccgggtc gaggggacga ttgaccatga acccggcggt gccctgtccg aaatcgaagt
     1561 gatcggccgc ctcgtcgacc gttgaatctg ccgggggtcc ccatgacgcg ttggttccgg
     1621 aagatgtccc gcctcatccc gttcgtggcc gcggtggccc tgctcgccgc gggatcatca
     1681 cccgcactgg cccagaacgg cttctacgcc gtcttctcac gtgacggtac cgacgtctgg
     1741 gcggtcggtg gaagcggcac cctctaccgc tcgttcgacg gcgggctcac ctggaccacg
     1801 cgcaccgtcg gcaccgacac gcatcgcggc gtgacggcca acggctggac cgacatcctc
     1861 gtatcggaca acggcaggat ccatcgttcg accgattcgg gcggcacctg gaacaccacc
     1921 accgtggccg gctcgccgtc gctgcgcggc gtgcagctgg tggatgccat gaacggctgg
     1981 atcgtcggcg cgtcgggcac gatcctccat accgtggatg gcggcgccaa ctggacgccg
     2041 caggcgagcg gcactgccgt gaagctcaac gcgctcaagt tcctcgacgc caccaccggc
     2101 tgggtggtgg gcgacggcgg caccatcctc aagaccaccg acggcggcgc cagctggacg
     2161 ccgctcacct ccggcaccac gaaggacctt tacggcgtcg acgccaccac gatcggcgcg
     2221 gtcgacccgg tcgtgtgggc cgtcggcaag gacgccaccg cgttgaagag cgtgaacggg
     2281 ggaacgaact tcacccccat cgacctcaag atcgactcga aggctgatgc gaatggcgtc
     2341 tatctcgctt cggccagcgc cgtcacgctc gtgggcggtg gcggcttcat ccgcgtctcc
     2401 gccgatggcg gggcctcatg gaatttccgc cagcacaaca ttcacggcga gatggacggc
     2461 gtctacatgg ccgccggcaa gggatgggcg gtcaaccgga gcaaccgcat cgtgctccgc
     2521 tcgggggatg ccggcgtcac gttcgcgccg cccgccggcg cgctggcctc ccgcacgctc
     2581 accctcaagc agtcggacgc cggggccatc atccgcggaa gcacgctggc ccagaacggc
     2641 cagaacaaga actcgttcta ctgcgtgctg agcgcgacgg tgtacaaatc gctcaaccgt
     2701 ggcgagacct gggccgccat cgggacgatc aacacgggca gcccctacgg cgcaccgagc
     2761 aagaccaatg ccttctacgt gcatccgagc gactcaacgc tgatgatcgc cgcggtcggc
     2821 gccaccgacc ggatcgtccg ctcgaccaac ggcggcgtca cctggaccac ggtgcccggc
     2881 tcggtgatcc cgttcaccga gtacggcgtc ccgctcgaga tggacgtcaa cgatcccaat
     2941 cacctgatcc tcggcgccga agaccag
//
LOCUS       DASVOD010000005         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_887367, whole genome shotgun sequence.
ACCESSION   DASVOD010000005 DASVOD010000000
VERSION     DASVOD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887367"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="VF720_00090"
     CDS             complement(<1..811)
                     /locus_tag="VF720_00090"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX7877774.1"
                     /translation="MPFARFRFLCALVVLGLLLASAPAMAQGTAGAYSLQIPPGARAE
                     GMGRSFTAIADDAFAPWWNPGGLAFLDGQNASLMHTKLVPDLADDVYFEYISYAQHLA
                     GWGGIAGTITYLSYGESQATDGSSGDLGTFSSWEIAPSVAIGTSITDNLGLGANLKYV
                     HLDLAPALSSTSGQAGKGSTFAVDIGALYRIPNAPINLALVVQNLGPDIALVDEEQAD
                     PLPRMLRVGGAWQVVNKNAHSLIFAFDADKLLLSDEGGAPPDTLFEPTWFDE"
     gene            complement(1002..>1835)
                     /locus_tag="VF720_00095"
     CDS             complement(1002..>1835)
                     /locus_tag="VF720_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7877775.1"
                     /translation="GAVLFDGRASVTGGRFEATFVVPVSLRGGARGRLRAYAYSQGVE
                     AVGAVDPLPIGGLSASAPVDTIAPSITIEVTAGTITPGDDLSILIEDQNGINVTQLFE
                     FRSIILSFLDESNLEAFRADVTEDFAYDAGSYQRGRVTTRVPDVAAGSYTIRLTATDN
                     FNNRGEARLTVSVGGAGEGGAVSNLLALPNPFESETTLVFDLEAATSQVRVLVYTVAG
                     RRVREWSVSGVAGENRLSWDGRDSRGDPVSNGVYLVKVAAQPADGGEEIDIVEPIVRL
                     R"
BASE COUNT          320 a          629 c          606 g          280 t
ORIGIN      
        1 tctcgtcgaa ccaggtgggc tcgaagaggg tgtccggagg cgcgccacct tcgtcgctga
       61 ggaggagctt gtcggcatcg aacgcgaaga tgaggctgtg ggcgttcttg ttgacaacct
      121 gccaggcgcc gccgacgcgc agcatgcgcg ggagtggatc ggcctgctct tcgtcgacca
      181 gcgcgatgtc gggcccgagg ttctggacga ccagggccag gttgatcggc gcgttgggga
      241 tgcgatagag ggcgccgatg tcgacggcga acgtgctccc cttgccggcc tgtccgctgg
      301 tggagctgag cgcgggtgcc aggtccaggt gcacgtactt gaggttggcg ccgaggccga
      361 ggttatcggt gatcgaggta ccgatggcga ccgacggtgc gatctcccac gagctgaagg
      421 taccgaggtc gcccgagctg ccgtcggtgg cctggctctc gccgtacgag aggtaggtga
      481 tcgttccggc aatgccgccc cagccggcga gatgctgggc gtacgagatg tattcgaagt
      541 agacgtcgtc ggccaggtcg ggaaccagct tcgtgtgcat cagcgaggcg ttctggccat
      601 ccaggaacgc cagtccgccg ggattccacc acggtgcgaa ggcgtcgtcg gcgatggccg
      661 tgaagctccg gcccatcccc tccgcccggg ccccgggcgg aatctgcaag ctgtaagcac
      721 ccgcggtccc ctgggccatg gccggcgcgc tggccagaag gagccccagg accacgaggg
      781 cgcacagaaa ccggaaacga gcaaaaggca ttgaaaaacc tcccgttaga cgatcggtcg
      841 gggcgcggat aggcggtgtg gtaccggcgc cggtaaccgg ggttcccaat cggaacctca
      901 aatgccgcac tatgctagca gaggatgcgc accccggcca gaactctctc ctggagaatc
      961 gagcgggtca aaagccccgc cgcgaagccc cctgtcgggc ctcagcgcag tcggacgatc
     1021 ggctcgacga tgtcgatctc ttctccgcca tcggcgggtt gggccgccac cttgaccagg
     1081 tagacgccgt tcgacaccgg gtccccgcgg gaatcacggc catcccagga gaggcggttc
     1141 tcgcccgcca cccccgacac gctccactcc cgcacccgac gtcccgccac ggtgtacacc
     1201 agcaccctga cctgggatgt ggccgcctcg agatcgaaaa cgagggtcgt ttccgattcg
     1261 aatggattcg gcagggccag caggttcgac accgcgccac cctcccccgc cccccccacc
     1321 gacacggtca gccgcgcttc cccacggttg ttgaaattgt ccgtggccgt caaccggatc
     1381 gtgtacgacc ccgccgccac gtccggtacc cgcgtcgtga cccgaccccg ctggtagctc
     1441 ccggcgtcgt aggcaaaatc ctccgtgaca tccgcccgga acgcctccag attcgactca
     1501 tccaggaatg acaggatgat cgagcggaat tcgaagagct gcgtcacgtt gatcccgttc
     1561 tggtcctcga tcaggatcga gaggtcgtct cccggcgtga tcgtcccggc cgtcacctcg
     1621 atcgtgatcg acggggcgat cgtgtcgacc ggcgccgagg ccgaaagacc gccgatcgga
     1681 agcggatcga ccgcgccgac cgcctcgacc ccctgcgaat aggcgtaggc gcgcaggcgg
     1741 ccccgggccc cgccgcgcag cgacaccggc accacgaacg tcgcctcgaa gcgccccccc
     1801 gtcaccgagg cccggccgtc gaagagaacg gcgcc
//
LOCUS       DASVOD010000006         2989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_365708, whole genome shotgun sequence.
ACCESSION   DASVOD010000006 DASVOD010000000
VERSION     DASVOD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365708"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VF720_00100"
     CDS             complement(<1..687)
                     /locus_tag="VF720_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7877776.1"
                     /translation="MRVLLAVIIKEFQQIWRDKRMVRMVVVAPILELVIFGFAANTEV
                     RHIPLVVVDQDRSAESRDLVNRFLASDYFDLAGTLDTTRDVEQWFVGGKAAVALVIAP
                     QFGREMMSAGRPRVQLLSDGSNSSVATIGLQYASGILGGYMEAKVGDMRAAAGLAREA
                     SGGLVIEPRVWYNPNLKSRWFYVPAVLAMVLMLMTLILSSMSVVKEKELGTMEQIIVT
                     PIRSWQLIVGK"
     gene            complement(724..1869)
                     /locus_tag="VF720_00105"
     CDS             complement(724..1869)
                     /locus_tag="VF720_00105"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7877777.1"
                     /translation="MRKLRAVAGKELRQARRDPLSLGLLLGIPTMMLLLYGYAVNFDV
                     RHVALSVEDNDRSRASRELVQAFVQSTYFDLKPERQPGETPEHLFERKRAQAILVIPD
                     DYGRELAAGRPATVQFLVDGTDATVAETILGYANALARDQNARLGARRMMPMAGGADG
                     TTPPPAPTLSVEPRVWYNPELRSSHFLVPGLIGFILMLTAVLSTALSVVREKERGTME
                     QIRMAPVAALELLVGKTVPYLVISLLATVLILIAAGFLFGVTIRGPLLDLGVAVILYL
                     LGGLGLGLFVSTLARTQAEAFQIGSMFSILPTFLLSGFIFPVKNMPAMMQVVSHIVPA
                     RYFLIILRGIILKGTGLGPYHGPLLALATYAVVMITLAGLRFGRRDA"
     gene            complement(1866..2837)
                     /locus_tag="VF720_00110"
     CDS             complement(1866..2837)
                     /locus_tag="VF720_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7877778.1"
                     /translation="MNDAIAIETRGLTRRFGNFTAVDRLDLTIRAGEIFCFLGSNGAG
                     KSTAIRMLCGLLRPTSGEARVLGIDVAARPEDVKRSIGYMSQRFSLYEDLSVIQNLHF
                     YGGVYGLYGKHLDSRIEWALEMAGLHGKEQRLTADLPGGWKQRLALGCAVLHEPKVLF
                     LDEPTGGVDPISRARFWDLIRDMAARGVTVMVTTHYMDEAEQCDRLALIHAGKLIALG
                     TVEELRSVFAGQAVLEVVVDDYLGALERLEADPRIRDASVFGTRLHVILDGEPGAAEA
                     VVRSILNAEQGQAAIRPVLPSLEDIFIHSIEMADAGNGAVAPAGKRS"
BASE COUNT          507 a         1019 c          988 g          475 t
ORIGIN      
        1 ctttcccacg atgagctgcc acgagcggat cggcgtcacg atgatctgct ccatcgtgcc
       61 gagctccttc tccttgacca ccgacatcga tgacaggatc agcgtcatca gcatcagcac
      121 catcgccagg accgccggca cgtagaacca gcgtgatttc aaattagggt tgtaccagac
      181 gcgcggctcg atcacgaggc cgcccgacgc ctcccgcgcg agtccggccg ccgcccgcat
      241 gtcacccacc ttcgcttcca tgtagccgcc caggatgccg ctggcgtatt gcaggccgat
      301 ggtggccacg gacgagttcg aaccgtcgga gaggagttgc acgcgtggcc tgcccgccga
      361 catcatctcg cgtccgaact gcggggcgat caccagcgcc acggcggcct ttccccccac
      421 gaaccattgc tccacgtcgc gggtcgtatc gagggtgccc gccaggtcaa aatagtcgga
      481 cgccaggaat cgattcacca ggtcgcggct ctcggcactc cggtcctggt ccaccaccac
      541 cagcgggatg tgccgcactt cggtgttggc cgcgaagccg aagatcacca gctcgaggat
      601 cggcgccacg accaccatgc gcaccatccg cttgtctcgc cagatctgct ggaattcctt
      661 gatgatgacc gcgaggagga cgcgcatgtc agctccagcc tcgcgggaac gaccgggatt
      721 tcatcaggcg tccctccggc cgaagcgcag ccccgccagg gtgatcatga cgacggcgta
      781 ggtggccagc gccagcagcg ggccatggta tggcccgagg cccgtccctt tcaggatgat
      841 gccgcgcagg atgatcagga agtaacgggc cggcacgatg tgcgacacca cctgcatcat
      901 cgccggcatg ttcttgaccg ggaagatgaa gcccgacagg aggaatgtcg gcaggatcga
      961 gaacatcgag ccgatctgga aggcctcggc ctgtgtgcgg gccagtgtcg agacgaagag
     1021 gccgagcccc aacccgccga gcaggtagag gatgacggcc actcccaggt cgagcagcgg
     1081 cccccgaatg gtcacgccga agagaaagcc ggccgcgatg aggatcagta ccgtggcgag
     1141 tagcgagatc accaggtacg gcacggtctt tcccacgagc aactccagcg ccgccaccgg
     1201 ggccatccgg atctgctcca ttgtgccgcg ctccttctcg cgcacgaccg acagcgcggt
     1261 ggagaggacc gccgtgagca tcaggatgaa gccgatcagc cccggcacga ggaagtggct
     1321 cgagcggagc tccgggttgt accagacgcg cggttccacg gagagggtgg gcgcgggtgg
     1381 gggagtggtg ccgtccgcgc cgccagccat cggcatcatc cgcctcgccc cgagccgcgc
     1441 gttctggtcg cgggccagtg cgttggcgta gcccaggatc gtctcggcca cggtggcgtc
     1501 ggtgccgtcc accaggaact gcacggtggc cgggcggccg gcggccagct cgcggccata
     1561 gtcgtccgga atcaccagaa tggcctgcgc ccgcttccgc tcgaacaggt gctcgggcgt
     1621 ctcgcccggc tggcgttccg gcttgagatc gaaataggtc gactgcacga aggcctgcac
     1681 cagctcgcgg ctggcccggc ttcgatcgtt gtcttccacc gacagcgcca cgtggcggac
     1741 gtcgaagttc accgcgtagc cgtacagcag gagcatcatg gtcgggatgc cgagcaggag
     1801 gcccagcgac agcggatcgc ggcgggcctg ccgcagctcc tttccggcta cggcgcggag
     1861 tttcctcatg agcgctttcc cgccggcgcg acggcgccgt tccccgcatc ggccatctcg
     1921 atcgaatgga tgaagatgtc ctcgagggag ggcaataccg gtcggatggc cgcctggccc
     1981 tgttcggcat tcaagatcga gcggaccacc gcttcggccg ctcccggctc accatcgagg
     2041 atcacgtgga gccgcgtgcc gaacaccgag gcgtcgcgga tgcgtgggtc ggcctcgagc
     2101 cgttccaggg cgcccaggta gtcatcgacc accacttcga gcacggcctg gccggcgaac
     2161 accgaccgga gttcctccac cgtgccgagc gcgatcagct tcccggcgtg gatcagggcc
     2221 aggcggtcgc actgctcggc ttcgtccatg tagtgcgtgg tgaccatcac ggtgacgccg
     2281 cgggcggcca tgtcacggat caggtcccag aagcgggcgc ggctgatcgg gtcgacgcca
     2341 ccggtcggtt cgtcgaggaa gagcaccttc ggttcgtgca gcaccgcgca accgagcgcc
     2401 agccgctgct tccacccgcc gggcaggtcg gccgtaaggc gctgctcctt gccgtgcagg
     2461 ccggccatct cgagcgccca ctcgatccgg gagtcgaggt gtttgccata cagcccgtac
     2521 acgccgccgt agaagtggag attctgtatg acactcaggt cctcgtagag gctgaagcgc
     2581 tggctcatgt atccgatcga cctcttcacg tcctcgggcc gggccgccac gtcgatgccg
     2641 agcacgcgtg cctcgccgga ggtggggcgg aggagcccgc acaacatccg gatggcggtc
     2701 gacttgccgg ccccgttcga gccgaggaag cagaagatct cgccggcccg gatcgtcagg
     2761 tcgagccggt cgactgcggt gaaattgccg aaacggcgcg tgagcccgcg cgtctcgatc
     2821 gcgatcgcgt cgttcacgac gcacctcccg cggcgcggat cttctccacg aagacttcct
     2881 cgagggagcg ttcggcggct ccccgcgtca gcgcctccgg cgtgttgagt gcgagcagtt
     2941 tgccccgctc gatgagcgcc agtcgctggc agcgctccgc ctcgtcgag
//
LOCUS       DASVOD010000007         2350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_626628, whole genome shotgun sequence.
ACCESSION   DASVOD010000007 DASVOD010000000
VERSION     DASVOD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626628"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2022)
                     /locus_tag="VF720_00115"
     CDS             complement(<1..2022)
                     /locus_tag="VF720_00115"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7877779.1"
                     /translation="MTPLAAPAAMPHHRAARGAAPIVRTLLVATTLLLLMHLPTSTVR
                     AADPAGTAPAGTTPAGTAPAGTTPADTTPVVATDWFKPLRDQQAAFAAQLPAAGASRV
                     TVDNLRAMMATGRLDEAAGYLGRVDGDPATVTLLSARVQLMRQDFDAVRGVLEPIVKS
                     RRALTDDEKDLVHAWFFAGDNSPEVAARTAGIDVSSHGDASVADLLAAGRLSYQMLNF
                     DRAESCFTSALAVAGSLESRAPFRAAALRGLGLVQYRRRDFDGAIAFHERAMKTLATP
                     EVLLASSETLIRLGRTDEAISCAEWAVKLNRYHDGAHYYLGNGYARKNYSELATAYPA
                     NFADAAGREAMTAADRLLENGRRDEARDAYVALRKAHPAWADVAIRLASFEFEEGRYA
                     AARDLCFAALDACPEYGRAHATLAKALEFQRFDIDIHRREYEARFAAMPMPDLPGIDR
                     YVANWKSLSPRHQKRVALSVEPWKNFLPVLLEGGCTHYIKPLYQRLSETPGLETLRDQ
                     RISYDSRLWDDVRGAGGYNTVTGLEDVERTIFDRYNTVLHELTHQVHGVTTADQGREI
                     QELYRKAKERDEATRNGFLSRYAGGSVYEYFAEGANALKSKKRDAYDSREEVYERLDA
                     MDPELMTLVRKFMALADLEPNYPIAYVNAGQDKVGKGEVDAGIVYFDKAL"
     gene            2046..>2350
                     /locus_tag="VF720_00120"
     CDS             2046..>2350
                     /locus_tag="VF720_00120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7877780.1"
                     /translation="MSDSRGRIIGHYASMPDGYRDWWAPVLDSMAAPALDALPHSGAP
                     ERWLDLASGTGIAARRLLKKLPTGSLVIAGDLVASMLERAREASPRIPVVRLDAARLP
                     "
BASE COUNT          346 a          796 c          820 g          388 t
ORIGIN      
        1 cagcgccttg tcgaaataga cgatccccgc atcgacctcg cccttgccga ccttgtcctg
       61 ccccgcattc acgtaggcga tcgggtagtt cggctcgaga tcggcgagag ccatgaactt
      121 ccggacgagg gtcatgagct cggggtccat ggcgtcgagg cgctcgtaca cttcctcgcg
      181 gctgtcgtag gcatcgcgct tcttcgattt gagcgcgttg gccccttccg cgaaatactc
      241 gtacaccgag ccgccggcgt agcgcgagag gaagccgttg cgcgtggcct cgtcccgttc
      301 cttggccttg cggtagagct cctggatctc gcggccctgg tcggcggtcg tcacgccatg
      361 cacctggtgg gtgagctcgt ggagcaccgt gttgtaacgg tcgaagatgg tccgttccac
      421 gtcctcgagg ccggtcacgg tgttgtagcc gccggcgccg cggacgtcgt cccagagccg
      481 gctgtcgtag ctgatgcgct ggtcgcgcag cgtttccagt ccgggcgttt ccgacaggcg
      541 ttgatacagc ggcttgatgt agtgcgtgca gcccccctcg aggagaaccg ggaggaaatt
      601 cttccatggc tcgacggaga gcgccacccg cttctggtga cggggcgaca ggctcttcca
      661 gttggccacg taacggtcga tccccggcag gtcgggcatc ggcatcgcgg cgaagcgggc
      721 ctcgtattcc ctgcgatgga tgtcgatgtc gaagcgctgg aactcgagcg ccttggccaa
      781 cgtggcgtgc gcccgcccgt actcggggca ggcgtccagg gccgcgaaac agagatcgcg
      841 cgccgcggca tagcgccctt cctcgaactc gaaactggcc agccggatgg ccacatccgc
      901 ccaggcggga tgcgccttgc gcagcgccac ataggcatca cgcgcctcgt cgcggcggcc
      961 attctccagc aaccggtccg ccgcggtcat tgcctcgcga ccggccgcgt cggcaaagtt
     1021 cgcgggatag gcggtggcca gctcactgta gttcttccgc gcatagccgt tcccgaggta
     1081 gtagtgggcg ccgtcgtgat aacgattcag cttgaccgcc cattcggcgc acgagatcgc
     1141 ctcgtcggtg cggcccagcc ggatcaacgt ttccgacgag gcaagcagta cttcgggcgt
     1201 ggccagcgtc ttcatcgccc gctcgtgaaa ggcgatcgcg ccgtcgaaat cgcggcggcg
     1261 gtactgcacg agcccgagtc cccgcaacgc ggcagcgcgg aacggcgccc tcgattcgag
     1321 cgatccggcc acggccagtg cgctcgtgaa gcacgattcg gcccgatcga agttcagcat
     1381 ctggtaggag aggcgcccgg ccgcgagcag gtcggccacc gacgcgtcgc cgtgcgatga
     1441 cacgtcgatc ccggccgtcc gcgcggccac ttcgggcgag ttgtcgccgg caaagaacca
     1501 cgcgtggacc aggtccttct cgtcgtcggt gagagcccgc ctcgatttca cgattggttc
     1561 cagcaccccg cgaaccgcgt cgaagtcctg ccgcatcagc tgcacgcgcg ccgagaggag
     1621 ggtgacggtc gctggatccc cgtccacgcg gccgaggtag ccggctgcct cgtcgagccg
     1681 gccggtggcc atcatcgccc ggaggttgtc caccgtgacg cgggatgccc cggcggccgg
     1741 aagctgcgcc gcgaacgcgg cctgctggtc ccggagcggc ttgaaccagt ccgtcgcgac
     1801 caccggagtc gtgtcggcgg gcgttgtgcc ggcaggcgcg gtgccggcag gcgttgtgcc
     1861 ggcaggcgcg gtgccggcag gatcggcggc tcgaacggtc gacgtgggga ggtgcatcag
     1921 gagcaggagc gtcgtggcaa cgagaagggt ccggacgatc ggcgccgctc ccctggcggc
     1981 gcggtggtgc ggcatggccg cgggggcggc gagcggagtc attgcggcag ggtaacatag
     2041 gccacatgag cgacagccgg ggccgcatca tcggccatta cgcctcgatg cccgacggat
     2101 accgtgattg gtgggcgccc gtgctcgact ccatggccgc cccggcgctc gacgcgttgc
     2161 cgcactccgg cgcgccggag cgctggctcg acctcgcctc cgggaccggc atcgcggcgc
     2221 gccgcctcct gaagaaactt cccaccggca gccttgtcat tgcgggagac ctggtggcgt
     2281 ccatgctcga gcgggcacgg gaggcatccc cccgaatccc ggtcgtccgg ttggacgcgg
     2341 cgcgcctccc
//
LOCUS       DASVOD010000008         3655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_939791, whole genome shotgun sequence.
ACCESSION   DASVOD010000008 DASVOD010000000
VERSION     DASVOD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_939791"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF720_00125"
     CDS             complement(<1..486)
                     /locus_tag="VF720_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7877781.1"
                     /translation="MSWDPAQYRLFSGHRLRPALDLLAAIPLEAPGTVVDLGCGEGRV
                     TRVLRDRWPDATLTAVDDSAAMLDVAAAELPHVDWRRGDIATWRPEAPVDCLFSNAAL
                     HWLPDHATLFPDLFRSVAPGGVMAIQMPRNFDQPSHTTIHATVAEGPWKARLTPLVRR
                     AP"
     gene            complement(483..2846)
                     /locus_tag="VF720_00130"
     CDS             complement(483..2846)
                     /locus_tag="VF720_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877782.1"
                     /translation="MTGCRALLYLLFHSSACLRYLAPSLLVVLGAVAAPRTAWAVPYD
                     GHPKILLHLAAPTVKSPCAAGKLTDCGSAVVAGSVGTPGGPFYFAYVLAAKGNLPDLA
                     GIQLGIRYQEGCVNGREDGNRIDVFGWTLCGTLEFPSSGFYSWPSPNSSNTITWDNGA
                     ACQTGETAVAGYFYVGCYGAADTLSVIPRPVDQLASVARCNTLETNLTSADLGRVVFS
                     PGGVSSGCNPCAGPCAPAPTYLSCGAGVDVTPPAPVTLSLRQRTNTSLTVQWQAPGDD
                     ANSGGPASAYDLRIFHGDPPYIFEMGTPVPGVPVPGTPGTVQFATATGLLPLETYTFL
                     LRTADEVPNWSGNPTNLFFATTLGGPPDPIAPAAITDLAQVSAGLHSIRLGWTATGND
                     GSTGTATSYEMRYSSSPITTEVEFANATPVTGMPSPTVAGSHQEKDVTGLTPGTTYYF
                     AIVALDVAFNRSPLSNSPACATLPQTETIPPAAITDLKALSATLSSVRLGWTAPGDDG
                     MMGTATLYDIRYSASPITAGNFASASQVGSEPAPQPAGTPQEMNVVLASSGTLYYFAI
                     KTIDNEDNVSGISNVVTRRTSDNSGARLLLHNRVTTTKNQCAQGQLADCGTAAVSNGA
                     LYPNLHFTYLLAGRFGEIGALQCGLQYDLGAADGDLNRRGIDIYSWTLCATQETPSTA
                     PAWPKPGSGNLITWNTATLCQNAGTAVAGYFYVGAYGVDLLQLTPRPSDGLARVYDCP
                     GTAQNLTADALGSAGFGRLGYNPCTGGQPSLPVQPTSWSQIKTLLGH"
     gene            complement(3042..>3655)
                     /locus_tag="VF720_00135"
     CDS             complement(3042..>3655)
                     /locus_tag="VF720_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HEX7877783.1"
                     /translation="LAPGAIYGNGIGEYLCVVIGKEHRLFAITFGAMAFSTFVFDTLD
                     VATRLGRYITQELFGWARSAGGAAATGLMVAIPAWILVTAEPGAYLKFWVLFGTSNQL
                     LAALTLLGVTVWLHRNRRPIWFTLPPMVFVMAITVWSLVKQIAGPIAAFREHGLRFDV
                     TMMNGVVSIALMILAVLLLVESAMAVKRGRRREDGDEPDTRAA"
BASE COUNT          637 a         1148 c         1310 g          560 t
ORIGIN      
        1 cggcgcgcga cgcacgagtg gcgtgagccg ggccttccat ggaccctccg ccacggtggc
       61 atggatggtc gtgtgcgatg gctggtcgaa gttgcgcggc atctgtatcg ccattacccc
      121 gcccggtgcc accgaccgaa acaggtcggg aaacagggtg gcgtgatccg gcagccaatg
      181 gagcgccgca ttggagaaga ggcaatccac cggcgcctcc ggccgccagg tcgcgatgtc
      241 gccccggcgc caatcgacat gagggagctc ggcggccgcc acgtccagca tggcggccga
      301 atcatccacc gcggtgagcg tggcgtccgg ccagcgatca cggaggacgc gcgtcacgcg
      361 cccctcgccg cagccgagat cgacgaccgt gccgggcgcc tcgagcggga tcgccgcgag
      421 gaggtcgagc gcgggccgaa gtcggtgccc ggagaagagc cggtactggg cggggtccca
      481 gctcaatggc cgagcagcgt cttgatctgg ctccacgacg tcggctggac ggggagcgag
      541 ggctggccgc cggtgcacgg gttgtaaccg agtcgaccga atccggccga gccgagcgcg
      601 tcggccgtca ggttctgcgc cgtgcccggg cagtcgtaca cgcgggccag accgtccgag
      661 ggacgggggg tcaactgcag caggtcgacg ccgtaggccc cgacgtagaa ataccccgca
      721 accgcggtgc ccgcattctg gcagagcgtc gcggtgttcc aggtgatcag gttgcccgaa
      781 ccgggcttcg gccaggccgg cgcagtggaa ggcgtttcct gtgtggcgca gagcgtccag
      841 gagtagatgt cgatgccccg acggttgaga tcgccgtccg cggcgccgag gtcgtactgc
      901 agcccgcact ggagggcgcc gatctcgccg aagcgcccgg caagcaggta ggtgaaatga
      961 agattgggat agagagcgcc gttgctgacc gcggcggttc cgcaatcggc cagctgaccc
     1021 tgcgcgcatt gattcttggt ggttgtcacg cgattgtgga gcaggagcct ggccccggag
     1081 ttgtcggatg tccgccgggt cacgacattg gagatgccgg agacgttgtc ctcgttgtcg
     1141 atcgtcttga ttgcgaaata gtagagcgtg ccgctcgacg ccagcaccac gttcatctcc
     1201 tggggagtgc cggccggctg gggcgccggc tcgctgccca cctggctggc cgaagcgaaa
     1261 ttcccggcgg tgatcggcga agcagaatag cggatgtcat acagggtggc cgtgcccatc
     1321 atgccatcat ctccgggcgc cgtccaaccg aggcgaacgc tcgacagggt ggcggagagg
     1381 gccttgagat cggtaatggc ggccggcggg atcgtttcgg tctgcggtaa cgtggcgcag
     1441 gccggcgaat tggagagagg cgagcggttg aaggcgacgt ccagcgcgac gatggcgaaa
     1501 tagtaagtcg tgcccggggt gagccccgtg acgtccttct cctggtggct gccggccacg
     1561 gtgggtgacg gcatgccggt cacgggggtg gcgttcgcga actcgacctc ggtcgtgatc
     1621 ggggaggagg agtagcgcat ctcgtacgac gtggcggttc ccgtggagcc gtcgttccct
     1681 gtcgcggtcc agccgagacg gatcgagtgg agccccgcgc tcacctgagc caggtccgtg
     1741 atcgcggccg gggcgatcgg atccgggggg cctccgagcg tggtcgcgaa gaacaggttc
     1801 gtcgggttgc ccgaccagtt gggcacttcg tcggcggtcc tgagcaggaa tgtataagtt
     1861 tcaaggggaa ggagcccggt ggccgtggcg aactggaccg ttccgggtgt ccccggcacc
     1921 ggaacgcccg gaaccggcgt gcccatttcg aaaatgtaag ggggatcgcc atggaagatg
     1981 cgcaggtcgt aggcggacgc cggccccccg gaattcgcgt catcgccggg ggcctgccac
     2041 tgcacggtca gcgaggtgtt ggtccgctgc cggagcgaca gggtaacggg ggccggcggc
     2101 gtgacgtcca cgccggcgcc gcatgagagg taggttgggg ccggtgcaca cggtcctgcg
     2161 cacgggttgc agcccgacga cacgccgccc ggcgagaaga cgaccctccc caggtccgcg
     2221 ctggtgaggt tggtttccag tgtgttgcag cgcgccaccg atgccagttg atcgactggg
     2281 cgcggaatca cgctcagggt gtccgcggca ccatagcagc cgacgtagaa gtatcccgcc
     2341 accgccgtct cgccggtctg gcaggcggcg ccgttatccc aggtgatcgt gttcgagctg
     2401 ttcggcgacg gccacgaata gaagccgctg ctcgggaatt ccagcgtgcc gcaaagggtc
     2461 catccgaaga cgtcgatccg gttgccgtct tcccgcccgt tgacgcaccc ctcctggtag
     2521 cggatcccca gctggatccc cgccaggtcg ggcaggtttc ccttcgccgc caggacgtag
     2581 gcgaaataaa aggggccacc cggggtgccc acgctccccg ccaccacggc cgaaccgcaa
     2641 tccgtcagct ttccggccgc gcagggcgat ttgacggtgg gtgccgccag gtggagcagg
     2701 atcttgggat gcccgtcgta cggcaccgcc caggccgtcc gtggggccgc gacggcgccg
     2761 aggacgacga gcagagaggg agcgaggtag cggaggcagg cagaagaatg gaacagcagg
     2821 tacaggaggg cgcggcaacc ggtcatggcg agggaccccc acaggtgagg tgtggaatgg
     2881 cgagacgagg ggatgcggga ctcggtctgc aggcgaccac aaccagtcta tcgaccgatt
     2941 cccggaccgg caagcggaat ggtcaatctt tgatatgcca aggagttcga gcaatagtcc
     3001 ccgccccgga atacccacca acaggttccg gaacctgttg atcaggccgc gcgggtgtcc
     3061 ggttcgtctc cgtcctcgcg tcgacgtccc cgcttcacgg ccatggccga ctcgacgagc
     3121 agcagcacgg ccaggatcat gagcgcgatc gataccaccc cgttcatcat cgtcacatcg
     3181 aaccgcaaac cgtgctcgcg gaaggccgcg atcggcccgg cgatctgctt caccagcgac
     3241 cagacggtga tggccatcac gaagaccatg ggtggcagcg tgaaccagat ggggcgccgg
     3301 ttgcgatgga gccagaccgt cacgccgagc agcgtaagcg ccgccagcag ctggttcgac
     3361 gttccgaaca gcacccagaa tttcaaatag gctcccggct cggcggtcac gaggatccag
     3421 gccgggatgg ccaccatgag gccggtggcc gccgcgccgc cggcgctccg ggcccagccg
     3481 aacagttcct gggtgatgta gcggccgagc cgggtggcca catccagcgt gtcgaacacg
     3541 aaggtcgaga aggccatcgc cccgaacgtg atggcaaaca gccgatgctc ttttccaatg
     3601 accacgcaca ggtactcgcc aataccgttt ccgtagatgg cgcccggcgc cagct
//
LOCUS       DASVOD010000009         3229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_783305, whole genome shotgun sequence.
ACCESSION   DASVOD010000009 DASVOD010000000
VERSION     DASVOD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783305"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /gene="moaC"
                     /locus_tag="VF720_00140"
     CDS             complement(<1..407)
                     /gene="moaC"
                     /locus_tag="VF720_00140"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEX7877784.1"
                     /translation="MAAAKKLTHVDSKGSARMVDVGAKPDTERRAMARGFVRMNAAAR
                     GALAENRLAKGDALAVARVAGIQAAKRTPDWIPLCHPLQLTHVRVEVVLDEKGGGVAI
                     EAEAATRGPTGVEMEALTAVAGAALTVYDMAKS"
     gene            646..1383
                     /locus_tag="VF720_00145"
     CDS             646..1383
                     /locus_tag="VF720_00145"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX7877785.1"
                     /translation="MDESVGLLAALTAGLISFVSPCVLPVVPAYLSFVSGMSIEEMKG
                     TGATGASRLRLFLNCLAFVAGFSTVFIMLGASATVVGGFLNENAKILSKVAGVIIIIF
                     GLHTMGVFKLPFLNYEKRFHQNQKSAGLLGSFLVGLAFAFGWTPCIGPILGTILGVAA
                     SSETVGKGILLLSVYSLGLGIPFLLAGLFVEAFFRVSSGVKKQFALIEKISGAFMIVV
                     GLMIFFDLFTMLSTWVSQALPWLSTIG"
     gene            1430..2011
                     /locus_tag="VF720_00150"
     CDS             1430..2011
                     /locus_tag="VF720_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7877786.1"
                     /translation="MKQVIHATRTATLAALLAFTLGATACAAEEKAAAPAAEPKTFVD
                     TSTLKPAGDFKLLDLNGKPKTLADYKGKVVIIDFWATWCGPCKASIPHLIELQKEYQA
                     QGLEVVGISLDTTGPKAVGSFATGQKINYTVLMGNDDVAAAYGGIKGIPVAFVVTQDG
                     KIYRRYVGYRDKKVYVSDIRALLGLGPAGTVKS"
     gene            2008..2316
                     /locus_tag="VF720_00155"
     CDS             2008..2316
                     /locus_tag="VF720_00155"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX7877787.1"
                     /translation="MSEDRRRLSDAEIGSALQGLPGWSLLAGKLHREFKFRDFVEAVG
                     FMTSAALVIQGMDHHPEWSNVYGTVRVDLVTHSASGVTATDVKLATKLNELADRLKPA
                     "
     gene            2355..>3229
                     /locus_tag="VF720_00160"
     CDS             2355..>3229
                     /locus_tag="VF720_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX7877788.1"
                     /translation="MAGVPVMSLISELGPILFLAAVLGLLAARLGQPPLLGYIAAGIV
                     AGSGAFGLIWHEETIRAFGDLGIVLLLFYLGLELSWHRMKPIAGRSVLLALTITGVPF
                     LVLVNILLLAGMRGITPAVIAFILSINSTAIAVQALEARGLFSGRLAGLVIGSNLVVD
                     ILAIIALTALGVGHGASDHNGGQSLGEVLLRTAGFVAACATVGLAVVPRALDAIHAHR
                     ASRLLMPVALIATGLMVAFAGERLGVPLSVGAFVAGILAAESKAREELVACLDPIKEL
                     FLGVFFVAVGFEVTLG"
BASE COUNT          494 a         1089 c         1022 g          624 t
ORIGIN      
        1 atcgacttgg ccatgtcgta cacggtgagc gccgcgccgg caaccgccgt gagagcctcc
       61 atctccacgc cggtggggcc gcgcgtggcg gcctcggctt caatggccac gccgcccccc
      121 ttctcgtcga gcaccacttc cacccgcaca tgagtcagct ggagggggtg gcagagcggg
      181 atccagtcgg gggttcgctt ggccgcctgg atgccggcta cccgggcaac ggccagtgca
      241 tcccccttgg cgaggcggtt ttcggccagg gcgccccggg cggcggcgtt catccggacg
      301 aagccgcgag ccatcgcccg acgttcggtg tcgggttttg ctcccacgtc gaccatgcga
      361 gcagaaccct ttgagtccac gtgagttagc ttctttgcgg ctgccatgct ggatccgtcg
      421 gtcgatagca gtgagcggga aggtgaagcc tatcaggcct tgccacggca cgaaagacgc
      481 caactcgcag tcccgagtga acctgcagcg gcgggcggtg tttcacccgt ccggtgccgg
      541 ccctctccct attaattcgg tcggaccaac gaatggcgca tgctacaatc cggcgcgagt
      601 ttggcttgta agttcaattg acatatagag gtacagcgaa aacacatgga tgagagcgtc
      661 ggactcctgg ccgcactgac cgccggattg atttccttcg tttccccctg cgtgctcccg
      721 gtggttccgg cttacctctc gttcgtgagc ggcatgtcga tcgaggagat gaagggaacg
      781 ggtgccacgg gggcttcccg gctgcgcctc ttcctgaact gcctggcctt cgtggcgggg
      841 ttctcgaccg tattcatcat gctcggcgcc tcggccaccg tcgtcggtgg cttcctgaac
      901 gagaacgcca agatcctgtc gaaggtggcc ggcgtcatca tcatcatctt cggcctgcac
      961 acgatggggg tcttcaagct ccctttcctg aactacgaaa agcgcttcca ccagaaccag
     1021 aagagcgcgg ggctgctcgg gtcgttcctg gtcggcctgg cattcgcctt cggctggact
     1081 ccctgcatcg ggccgatcct cggcaccatt ctcggcgtgg cggccagttc ggaaaccgtc
     1141 gggaaaggga ttctccttct gtcggtctat tcgcttggcc tcggcatccc gttcctcctg
     1201 gccggtctct tcgtcgaagc gttcttccgc gtctcgagcg gagtcaagaa acagttcgcc
     1261 ctgatcgaga aaatcagcgg cgccttcatg atcgtcgtcg gtctcatgat cttcttcgat
     1321 ctcttcacca tgctgtcgac gtgggtgtca caggccctgc cgtggctctc caccatcggt
     1381 tgatacaggc gcgcggccgc acctggccgg gtactcggag gatcagttca tgaagcaagt
     1441 catccacgcg acgcggaccg ccacgctggc ggcccttctt gccttcaccc tcggcgctac
     1501 ggcgtgcgcc gccgaggaaa aggcggccgc accggccgcc gaaccgaaga ccttcgtcga
     1561 cacctccacg ctcaagccgg ccggtgattt caagctgctc gacctgaacg gcaagccgaa
     1621 gacgctggcc gactacaagg gaaaggtcgt catcatcgac ttctgggcca cctggtgcgg
     1681 gccgtgcaag gcctccatcc cgcatctcat cgagctgcag aaggagtacc aggcgcaggg
     1741 tctcgaggtc gtggggatct cgctcgacac cacgggtccc aaggcagtcg gctcgttcgc
     1801 caccgggcag aaaatcaact acaccgtcct gatggggaac gacgacgtgg ccgcggccta
     1861 cggtggcatc aagggaatcc cggtggcgtt cgtcgtcacc caggacggca agatctaccg
     1921 ccggtacgtc ggataccgcg acaagaaggt gtacgtcagc gacatccgcg cgctcctcgg
     1981 actcgggccg gccggcaccg tcaagtcgtg agcgaggatc gccggcgtct ttccgacgcc
     2041 gagatcgggt cggcgctcca gggactgccg ggctggagtc ttttggccgg caagctgcac
     2101 cgcgaattca aattccgcga cttcgtcgag gccgtcggct tcatgacgag cgcggcactg
     2161 gtgatccagg ggatggatca tcaccccgaa tggtccaacg tgtacgggac tgtccgtgtc
     2221 gacctggtca cgcattccgc ctccggcgtc accgccaccg acgtgaagct ggccacgaag
     2281 ctcaacgaat tggccgatcg actgaagccc gcctgacgat gcgctgagcg cgcgcccgac
     2341 gaggccccgt cgcgatggcc ggggtgcccg tcatgtcgct catttccgaa ctcggtccca
     2401 tcctcttcct cgcggcggtg ctcggcctcc tggccgcgcg cctgggacag ccgcccctcc
     2461 tcggctacat cgccgccggc atcgtggccg gctccggcgc cttcggcctc atctggcacg
     2521 aggagacgat ccgggcgttc ggcgacctcg ggatcgtcct cctcctcttc tacctcggcc
     2581 tcgagctctc ctggcaccgg atgaagccga tcgccggccg cagcgtgctg ctcgcgctca
     2641 ccatcaccgg cgttccgttt ctcgtcctgg tcaacatcct cctcctggcc ggcatgcgcg
     2701 gaatcacgcc cgcggtgatc gccttcatcc tgtcgatcaa cagcacggcc atcgccgtgc
     2761 aggcgctcga ggcccgcggg ctcttttccg gtcggctggc cggactcgtc atcgggtcga
     2821 acctggtggt ggatatcctg gccatcattg ccctgacggc gctcggtgtg gggcacggcg
     2881 catcggatca caacggggga cagtccctgg gtgaggtgct gctgcgcacg gcgggatttg
     2941 tggccgcctg tgccacggtg ggccttgcgg tggtgccccg cgcactcgac gccatccatg
     3001 cccaccgggc ctcacggctc ctcatgccgg tggcgctcat cgccaccggg ctcatggtgg
     3061 ccttcgcggg ggaacgtctg ggcgtgcccc tctcggtggg cgcgttcgtg gccggcatcc
     3121 tggccgccga gtcgaaggcg cgggaagagc tggtggcctg cctcgatccg atcaaggaac
     3181 tgtttctcgg cgtcttcttc gtggctgtcg gcttcgaggt aacgctggg
//
LOCUS       DASVOD010000010         4226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_992068, whole genome shotgun sequence.
ACCESSION   DASVOD010000010 DASVOD010000000
VERSION     DASVOD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992068"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF720_00165"
     CDS             complement(<1..275)
                     /locus_tag="VF720_00165"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX7877789.1"
                     /translation="MAGTLFRSVKKGGSLLVTGALFLGILVMINVAAGRLAWRSDWTG
                     ENRYSLSPQTHQILKSLDRDVSIMAFLQTNDPDQDELRRLFDEYASS"
     gene            complement(280..1053)
                     /locus_tag="VF720_00170"
     CDS             complement(280..1053)
                     /locus_tag="VF720_00170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7877790.1"
                     /translation="MRGFTALLKRELSLYFIPPVGYVVLSLFLFSVGLTWYGALTRFV
                     EYARQAGAGLGSQNPVDLHLQLMTPWFFNLAIIAVFLLPLLTMRLMAEERRQGSIELL
                     LTWPLSDFQVVMAKYLAALAFWGLMLLATAWTTLVLFRLGNPDPGPMLAGYLGVFLYG
                     GALIALGLMVSSLTENQIVAAMVSFVLFFLLWSFEPLSVATTGWFSAFLAGAAVLMHL
                     KPMTQGVVDMKDVLFFLTVAGFGIGVAHQALAARRWSGS"
     gene            complement(1053..1988)
                     /locus_tag="VF720_00175"
     CDS             complement(1053..1988)
                     /locus_tag="VF720_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7877791.1"
                     /translation="MIEVEGLTKSYGPTRAISDLSFSVAKGEILGFLGPNGAGKTTTM
                     RILTGFMPPAAGRVTIDGLDAANDSLAIRRRIGYLPESVPVYRDSSVADYLGLVADVK
                     GVPRRGREKTLASVMEQCGLTGVRHRIVGRLSRGYRQRVGLAQALVNDPAVLILDEPT
                     VGLDPAQVVEVRDLIRGLAGTRTVILSTHILPEVSQIASRILILDKGRIVAEGSPAEL
                     MRRLQGRPSWRVRVGGDTGLAQRLLAAVPGVLAVRVETPGNDGGTLFVETDDVADRRA
                     AISRALIDGGSSLLELASADLTLEEIFLRIVGKEG"
     gene            complement(1999..3168)
                     /locus_tag="VF720_00180"
     CDS             complement(1999..3168)
                     /locus_tag="VF720_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013321679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7877792.1"
                     /translation="MTAKPRGSIKAARFTESVIREMTRLAQLHGALNLAQGFPDFPAP
                     AALKEAAIQAIRDDVNQYAITWGAKSCREAIARKFARYGGFPIDPEREITVTCGATEA
                     MIATLLAVVDPGDEVIVFEPFYENYGPDAILSGAVPRFVKLRAPDWTFDPAELAAAFN
                     DRTRAIIINTPNNPTGKVFDAAEMETIAALCRRHDVLAVTDEIYEHILYDGAEHVVMA
                     SLEGMRERTVTINALSKTYSVTGWRVGYIIAPPDLTAAIRKTHDFLTVGAPAPLQAAA
                     ATALDLPDSYYEELRKGYHQRRDILYDALGTAGLKANRPRGAYYIMADFGGVGFDGDD
                     TAFAHHLIREIGVAPVPGSSFFNDPKDGATIIRFCFPKQHATLTAAGERLQRLTR"
     gene            complement(3165..>4226)
                     /locus_tag="VF720_00185"
     CDS             complement(3165..>4226)
                     /locus_tag="VF720_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7877793.1"
                     /translation="IAAGSHRSLRLLPGHGVLGMTLALVVALAVLDIINIPFGLIAWQ
                     KARAVGLSTQGLGDWWLDWGKGVLLDWPITALVAAVLFFLIDRFPRGWPLPATLLAAV
                     GGAVMILLVPLVVDPLFNKFTPLTDARLTASFLDLAEKGGVPAREVLVSDASRRTTAV
                     NAYFTGFGPTRRIVVYDTMVEKLTPRESELVLAHEVGHWSHNHIVLGLLLGTIGLGLA
                     LLAGAAVYGHMVRNGSFGITGALDPAAVPVVILLYWLGLFLAMPIENGISRRFEKQAD
                     EFALELTRDAATQVSVERSLARTNLPDLVPPPFIEALMYTHPKTLDRIVHAESWAAAH
                     GIPLAPAAIDTPDTRRDTP"
BASE COUNT          714 a         1390 c         1505 g          617 t
ORIGIN      
        1 ctcgacgagg cgtactcgtc gaagaggcgg cgcagctcgt cctggtccgg atcgttcgtc
       61 tggaggaagg ccatgatcga cacgtcgcgg tcgagcgatt tcaggatctg gtgcgtctgc
      121 ggcgacaggc tgtagcggtt ctcccctgtc cagtccgagc gccaggccag ccgtcccgcg
      181 gccacgttga tcatgacgag gatcccgagg aagagggcgc cggtgacgag gaggctcccc
      241 cccttcttca cgctccggaa gagtgtgccg gccatcgcgt cagctccccg accagcggcg
      301 cgcggccagc gcctggtggg ccacgccgat gccgaaaccg gccacggtga ggaaaaagag
      361 gacgtccttc atgtccacca ccccctgagt catcggcttc aggtgcatga gcaccgccgc
      421 gcccgccagg aaggcggaga accagccggt cgtggccacc gagaggggct cgaagctcca
      481 gaggaggaag aagaggacga acgacaccat ggccgccacg atctggtttt cggtgaggct
      541 cgacaccatg aggccgagcg cgatcagcgc gcccccgtag aggaacacgc cgagatagcc
      601 ggccagcatc ggccccggat cgggattgcc gaggcggaag aggacgagcg tggtccacgc
      661 cgtggccagg agcatgagtc cccagaacgc cagggcggcc aggtacttgg ccatcaccac
      721 ctggaagtcc gagagaggcc aggtgaggag gagctcgatg ctcccctgcc ggcgctcctc
      781 ggccatgagg cgcatggtga ggagcggcag caggaagacg gcgatgatgg ccaggttgaa
      841 gaaccagggg gtcatgagct gcaggtggag atcgaccggg ttctgcgagc ccagcccggc
      901 gccggcctgg cgggcgtact ccacgaagcg ggtcagcgcc ccgtaccagg tgagccccac
      961 gctgaagagg aagagggaaa gcaccacgta gccgaccggc gggatgaaat agagcgagag
     1021 ttcgcgcttg agcaacgccg tgaagccgcg catcacccct ccttcccgac gatccggagg
     1081 aagatctcct ccagcgtgag gtcggcggag gcaagttcga gcagcgagga gccgccgtca
     1141 atcagggccc gcgagatggc ggcgcggcga tcggccacgt cgtcggtttc cacgaagagc
     1201 gtgccgccgt cgttcccggg cgtttccaca cgcacggcca gcacgccggg aaccgccgcc
     1261 aggaggcgct gggccagccc ggtatcgccc cccacgcgga cgcgccacga agggcgaccc
     1321 tgcaggcgcc gcatcagctc ggccggcgat ccctccgcca cgatccgccc cttgtcgagg
     1381 atcaggatgc ggctggcgat ctggctgact tccggcagga tgtgggtgga gaggatgacc
     1441 gtgcgggtac cggccaggcc tcggatcagg tcgcgcactt ccaccacctg cgccgggtcg
     1501 aggccgaccg tcggctcatc gaggatgagc acggccggat cgttcaccag cgcctgggcc
     1561 agccccaccc gctgtcgata gccgcgcgaa aggcgcccca cgatgcgatg gcgcacgccc
     1621 gtcaggccgc actgctccat caccgaggcg agggtcttct cgcggccgcg gcgcggcact
     1681 cctttcacgt cggccacgag gccgaggtaa tcggccacgc tcgaatcgcg gtagaccggc
     1741 acgctttcgg ggagataacc gatccggcgg cggatggcca gcgaatcatt ggcggcatcg
     1801 aggccgtcga tcgtgacgcg gcccgcggcg ggcggcatga agccagtcag gatccgcatg
     1861 gtggtggtct tccccgcgcc gttcgggccc aggaagccga gaatttcgcc tttggccacc
     1921 gaaaagctga ggtcggaaat ggcgcgtgtg ggaccgtacg acttggtgag cccctcgact
     1981 tcgatcatct cgggccggct accgggtgag gcgctgcagc cgttcgccgg ccgcggtgag
     2041 cgtggcgtgc tgcttcggga agcagaagcg gatgatcgtg gcgccgtcct tcgggtcgtt
     2101 gaagaaggag gagcccggca cgggcgccac gccgatttcc cgtatgaggt gatgggcgaa
     2161 ggccgtgtcg tcgccgtcga agccgactcc gccgaagtcg gccatgatgt agtaggcgcc
     2221 gcgcggccgg ttggccttga gccccgccgt gccgagagcg tcgtacagga tgtcgcggcg
     2281 ctggtgatat cccttccgca actcctcgta ataactgtcc ggcaggtcga gcgcggtggc
     2341 cgcggcggcc tgcagcggcg ccggcgcgcc gacggtgagg aagtcgtgcg tcttccggat
     2401 ggccgccgtg aggtcgggcg gggcgatgat gtatcccacg cgccagcccg tcacgctgta
     2461 ggtcttcgaa agagcgttga tggtgacggt gcgctcccgc atcccctcga gggaggccat
     2521 caccacgtgc tcggccccgt cgtacaggat gtgctcgtag atctcgtcgg tgaccgccag
     2581 tacatcgtgg cgccggcaga gcgccgcgat cgtctccatc tcggccgcgt cgaacacctt
     2641 gccggtcggg ttgttgggcg tgttgatgat gatggcgcgc gtgcggtcgt tgaaggcggc
     2701 ggccagctcg gcggggtcga acgtccagtc gggcgcgcgg agcttgacga agcgcgggac
     2761 ggcgcccgac aggatggcgt cgggaccgta gttctcgtag aacggctcga agacgatcac
     2821 ctcgtcgccg ggatcgacca cggccaggag cgtggcgatc atcgcctcgg tggcgccgca
     2881 ggtcaccgtg atctcccgct cgggatcgat cggaaagccc ccgtagcgcg cgaacttccg
     2941 ggcgatggcc tcccggcacg atttggcccc ccaggtgatc gcgtactggt tcacatcgtc
     3001 ccgaatggcc tggatggccg cttccttgag tgcggccggc gccgggaaat ccggaaaccc
     3061 ctgcgccagg ttcagggcgc catggagctg ggccagccgc gtcatctcgc ggatgaccga
     3121 ctcggtgaag cgggccgcct tgatcgagcc gcgcggcttc gccgtcatgg cgtatctctc
     3181 cgggtgtcgg gggtatcgat ggcggcgggt gcgaggggaa ttccgtgggc cgccgcccag
     3241 gactcggcat gaacgatccg gtccagcgtc ttgggatgag tatacataag cgcctcgatg
     3301 aacggcggcg gcaccaggtc cggcaggttc gtgcgggcca ggctccgctc caccgacacc
     3361 tgcgtggccg cgtcgcgcgt gagctccagc gcgaactcgt cggcctgctt ctcgaagcgg
     3421 cgcgagatgc cgttctcgat cggcatcgcc aggaagaggc cgagccagta aaggaggatc
     3481 acgacgggca ccgccgccgg atcgagtgcc ccggtgatgc cgaacgagcc gttccgcacc
     3541 atgtgcccgt acaccgccgc gcccgccagc agcgcgaggc cgagcccgat cgtgccgagg
     3601 aggagtccga ggacgatgtg gttgtgcgac cagtgcccca cctcgtgggc cagcaccagc
     3661 tccgattcgc gcggcgtgag tttctccacc atcgtgtcgt acaccacgat gcggcgcgtc
     3721 gggccgaagc cggtgaagta ggcgttcacc gcggtggtgc gcctgctggc atcgctcacc
     3781 agcacttcgc gggccggcac gccccccttc tccgccagat cgaggaagga cgcggtaagc
     3841 cgggcatcgg tgagcggcgt gaacttgttg aagagggggt ccaccaccag cggcacgagg
     3901 aggatcatca ccgcaccccc caccgccgcc agcaacgtgg ccggcagcgg ccagccccgc
     3961 gggaaccgat cgatcaggaa gaagaggacg gccgccacca gcgccgtgat cggccagtcg
     4021 agcagcacgc ccttccccca gtcgagccac cagtcgccga gcccctgcgt ggagagaccg
     4081 acggcccggg ctttttgcca cgcgatgagg ccgaacggga tgttgatgat gtcgagcacg
     4141 gccagcgcga ccacgagggc cagcgtcatc ccgaggacgc cgtgcccggg gagaaggcgc
     4201 agactccggt ggctgcctgc ggcgat
//
LOCUS       DASVOD010000011         8922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_626790, whole genome shotgun sequence.
ACCESSION   DASVOD010000011 DASVOD010000000
VERSION     DASVOD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626790"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VF720_00190"
     CDS             <1..820
                     /locus_tag="VF720_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7877794.1"
                     /translation="KAPFLALNCAAIPEQLLESELFGHEKGAFTGAVRSRPGKFEQAH
                     HGTLFLDEIGEMPAAMQAKLLRVLESGEVERVGGPGSRRVDVRVVSATNVDLARAVTR
                     GRFRDDLYHRLNVVPVRLPALRERPDDFPVLVDHLLERLSQVHGKRPRRLAPPALARL
                     AAREWPGNVRELRNALERRVILSAGAVIDELAVDAFLSPAGLVPVETTTDTAVSEGGS
                     LRDRLRVVERDIIARCLDANGGNVTATAKALGLERSHLHRKIRELGVDRDADAT"
     gene            complement(813..2090)
                     /locus_tag="VF720_00195"
     CDS             complement(813..2090)
                     /locus_tag="VF720_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7877795.1"
                     /translation="MTGRLHARAVGAALLGFLMLWGLAACGGSGGAPKGRVLLIGLDG
                     AEWNLIRPMMAAGEMPNLKALVDRGVSGDLKSLVPLQKSPAIWTTIITGKPPEEHGIR
                     GFVDQVGGKPLTQNIRRVKALWNILSSTGRKVGVVGWLMSWPAESVNGFVVSDYVQYQ
                     AARNQRLENRTFPPALYDSVANLVRDWETVSWTEVSAFLSQPFDPATGDTTLTSRLAS
                     IKWMVAADQGFADIADRLGRKEPWDFLAVYLRSMDTMGHLFWNYQNPEAHPPQLVDAA
                     MTPYARDIMRKNYRWIDGQIGRLVALADDRTTIMVCSDHGFVGGGGGGVRDHRVEGIL
                     VMAGPHIGKGEITGATVFDVTPTLLALFGLPKADDMRGKVLWTAFDGTIRPEQFQHAL
                     PTYETGDGQAGEGVASPVDKELIERLKSLGYIK"
     gene            complement(2087..2929)
                     /locus_tag="VF720_00200"
     CDS             complement(2087..2929)
                     /locus_tag="VF720_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX7877796.1"
                     /translation="MTAPVTVGVALSGGTAKTIAHIGVLEALEDAGIRVSCITGTSGG
                     AIIGALYAAGFSIPELKALARGVRWNKLARLSFPRLGFLSNDTVHRFLTDLLGSRTFE
                     QLRIPLAIVGTNLLTGDRAIFREGSVALAARVSSTIPHLFAPVEIGDDMYVDGGMVEF
                     LPVATLRDTFDPDVRVGVNLGLRGRLPRPRNFIQVSVVVGTVVARQTVRYNESLADVV
                     VRPDTSLFHPFDLEASQGLIEVGYQAVVEELDALAAAIRRHDPSFEAKPLGSGGLGLA
                     GESR"
     gene            complement(2926..3522)
                     /locus_tag="VF720_00205"
     CDS             complement(2926..3522)
                     /locus_tag="VF720_00205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7877797.1"
                     /translation="MDKTTTEFDILEYQKALGERIRRFRRDLQLTQMDLARQVGVTNG
                     QISTIERGLSAPSIGTLRRISEAMGIAMVYFFDQPGQRDVMVVRRGDRTRLTSPTSPE
                     ILEVLAGSRTMTALQVGLNPGQSCRRPGHDSASELFLIVQQGQIEIEVESQTMALKSG
                     DSARLDGRSGRSYICQGESPALVLEVRSESTTNFAPEV"
     gene            3762..4730
                     /gene="pfkA"
                     /locus_tag="VF720_00210"
     CDS             3762..4730
                     /gene="pfkA"
                     /locus_tag="VF720_00210"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX7877798.1"
                     /translation="MNRIGILTSGGDAPGMNAAIRAAVRTASVRGVEMVGITEGYTGL
                     LAGLVHTLGPRAVSNTIQRGGTILGTSRCEEFRNPEGRRLGAERLRHAGIDGLIVIGG
                     DGSFRGADRLATECGIAVIGVPGTIDNDISGTDATIGYDTAVNTAIDAIDKIRDTAAS
                     HSRIFFIEVMGRHAGFIALEAAIGGGAEEVILPEEEVTIELVCERLRDGMTHGKSSSI
                     VIVAEGHPLGGAVAIAERVEAILPVDTKTTILGHIQRGGRPTARDRVLASRLGAAAVD
                     RLLASETGVMVGEVAGSVMTTPLPDTWNQRKPLDAGLLRLARELSA"
     gene            4727..6235
                     /locus_tag="VF720_00215"
     CDS             4727..6235
                     /locus_tag="VF720_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877799.1"
                     /translation="MIRTPPHRIGLVLLAALLAVTVSTMSASGAGTARVTFDHLTWAP
                     EGSALAVEARFESSDGVATDTLLVDLTEGSVATRQFRPSHFALDAGGRRIVAAGRYAM
                     VTASLDDPDRLERLSGRDPARQEVVRLSFTARGDSVLLLSRTRGSVRYELSAWPWPTG
                     RQAMRLITSSRTDAIRAFNAAASATRPLAVEEDPLPHLYRQIGSNLFHLEASQTPVPG
                     VPSFGLFNLFHRDMERGQSKLLATNVAPYDLLASPDSAWVAVAGHRAVADYGGRLVKC
                     LWIASTDGAIFFSVAPTGERSYHPADVRAMCWVSRKLWYSTPDGLFSVDPATGRAVRI
                     TLGPPPPVWSAQLAPSTPLWTLLSRSSAIDTAEAVRERERLRKDGWPAWVTTWQGDRF
                     RAAIGSAVDEASLAPLSKRLVEAGHTGFSAARVDPVASGSPFPWGMVKSPVDSREAYL
                     VSVGPPGFTAGEIWLTELQGARQIRLVKAMLVGHDLPPGSGYVPEPPEPGPR"
     gene            6354..8072
                     /locus_tag="VF720_00220"
     CDS             6354..8072
                     /locus_tag="VF720_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877800.1"
                     /translation="MPRRTAPLPPLLRLLVALVIVGTPIFAAIVDGAPRKSPRRRSFT
                     PDQMQRLRAADDGLRILTRNARLSETYISPKGDGVQDVTLISYEVVAPDSITMFVYLD
                     TAAWGTVEHPFRGIIRDYELRPPGPTVIPFNGNIGTGPDAARLDGVYGVIIRAIGDTG
                     RVEVDTLMLVIDRVSPVLQSVTTLGGLSSYRNGETVVIQIQADQPGYTASANFSGIDS
                     DPINRTEVLELGNGRYEIRHVISDTNTKADTSNVLVPVRLVDLAGNFTNYNALRLCLS
                     NHPPVLLSAVTLNAPEGAYKGESLIQIETTWQSVDTALTVRADFKGIDTAYDSTRYVT
                     SRLPGNRYHSTYRLSDANQKPDGSYLVPIVASDHGCGVSPAALVTIALDTEAPPLPVF
                     DAVPTGVREPAYTLRGTANGSVRVLVLQNEALVDTFQVNPDGRFEVPVTLVPGNNRFT
                     AEGLDTAGNKTVRSVPVQIVYVTGGYVTIPLPFRPGNTFQVGASQPASGVRIELWTLG
                     GDLARVLTNESPAEVYNISWDGRDEAGERLNSGPLIAIITTDFTDGSSATEKRAMVLA
                     SGAGTP"
     gene            8102..>8922
                     /locus_tag="VF720_00225"
     CDS             8102..>8922
                     /locus_tag="VF720_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877801.1"
                     /translation="MWPLVAFAALGLLLSPKGVRAGHTTTAFADIEPGPRIMAMGGAG
                     AGLANDPTAAWWNPAGLYFMRGTQGVVTYDDLYGEGLIQRNYLSVAWKKVHYEPRFDD
                     NVLTLERDLNRGSAFGFSVSSLFLDLDESSYSEFMPTLSYAAGVGGGVGLGASLSFLK
                     ASSDVVGVGSTGYSATVGVSFPLPWDARFGYAARNLFSSLSPEEGAGEKLPITSAVGV
                     SAPIGKAGLVAADATFSDGDSRFGAGAEYWFFNHHLAGRAGLRQFDGAVESRVVPT"
BASE COUNT         1427 a         3134 c         2909 g         1452 t
ORIGIN      
        1 caaggcgccc ttcctggccc tcaactgcgc ggccatcccc gaacaactgc tggagagcga
       61 actgttcggc cacgagaagg gcgccttcac cggcgccgtg cgaagccgtc cgggcaagtt
      121 cgagcaggcc caccacggca cgctcttcct ggacgagatc ggcgagatgc cggccgccat
      181 gcaggccaag ctcctccgcg tgctggaatc aggtgaggtg gagcgcgtgg ggggacccgg
      241 cagccggagg gtggatgtcc gcgtcgtctc ggccacgaac gtcgacctgg cccgggccgt
      301 cacgcggggt cgattccgcg acgatctcta ccatcgcctc aacgtggtgc cggtccgtct
      361 cccggcactc cgcgaacgcc cggacgattt ccccgtgctc gtcgatcacc tgctcgagcg
      421 cctgtcgcag gttcatggga aacgcccgcg gcggctggct cctccggccc tggcccgcct
      481 ggcggcacgg gagtggcccg ggaacgtgcg ggagcttcgg aacgcgctgg aacggcgggt
      541 catcctgtcg gcgggcgccg tcatcgacga actcgctgtc gacgccttcc tctccccggc
      601 cggcctcgtt cccgtggaaa cgaccaccga cacggcggtg tcggagggag gttcgctgcg
      661 cgatcgcctt cgcgtcgtgg agcgcgacat catcgcgcgc tgtctggatg ccaacggcgg
      721 aaacgtcacc gccaccgcga aggccctggg cctcgagcgg agccacctgc accggaagat
      781 ccgcgagctg ggcgtcgacc gcgacgccga cgctacttga tgtagccgag cgacttcagt
      841 cgctcgatga gttccttgtc gaccggtgag gccacgccct cgcccgcctg accgtcgccc
      901 gtctcatagg tgggaagcgc atgctggaac tgctccgggc ggatcgtgcc gtcgaaggcc
      961 gtccagagca ccttgccccg catgtcgtcg gctttcggca ggccgaaaag cgccagaagg
     1021 gtcggcgtca cgtcgaaaac ggtggcgccg gtgatctcac ccttgccgat gtgcggcccc
     1081 gccatgacca ggatcccctc gacccgatga tcacgcacgc cgcccccgcc gcctcccacg
     1141 aaaccgtgat cggagcagac catgatggtc gtcctgtcgt cggccagtgc cacgagtcgg
     1201 ccgatctggc cgtcgatcca ccggtagttc ttccgcatga tgtcgcgcgc atagggcgtc
     1261 atggcggcat ccaccagctg cggcgggtgc gcctcgggat tctggtagtt ccagaagagg
     1321 tgacccatgg tgtccatcga tcggagatac accgccagga aatcccacgg ctccttccgg
     1381 cccagccggt cggcgatgtc ggcaaaaccc tggtcggcgg ccaccatcca cttgatcgag
     1441 gccagacggc tggtcagcgt ggtgtcgccg gtggcgggat cgaagggctg cgacaggaag
     1501 gcgctcactt ccgtccagct caccgtctcc cagtcacgga ccaggttggc cacgctgtcg
     1561 tagagggcgg gcggaaaggt ccggttctcg aggcgctggt tccgcgcggc ctggtactgc
     1621 acgtagtcgc tcaccacgaa gccgttcacc gactcggcgg gccacgacat caaccacccg
     1681 accacgccca ctttccggcc cgtcgaggag aggatgttcc agagcgcctt cacccgccgg
     1741 atgttctgcg tgaggggctt cccccccacc tggtcgacga agccacggat gccgtgttcc
     1801 tccggcggct tgccggtgat gatggtggtc cagatggccg gtgacttctg gagcggcacc
     1861 agcgacttga ggtctccgga aacgccccga tcgaccagcg ccttcaagtt cggcatttca
     1921 ccggccgcca tcatggggcg aatgaggttc cactcggcgc cgtcaaggcc gatgagcagc
     1981 acccgcccct tcggcgcccc gccggatcca ccgcacgccg cgaggcccca cagcatgagg
     2041 aatccgagga gggcggcccc caccgcgcgc gcgtgcaggc gcccggtcat cgggactccc
     2101 cggccagccc caatccgccc gagccgagcg gcttggcctc gaacgaggga tcgtggcggc
     2161 gtatcgccgc ggccagggcg tcgagttcct ccaccaccgc ctggtagccg acttcgatca
     2221 gaccctgcga ggcctcgaga tcgaacgggt ggaagagact cgtgtccggg cgaaccacca
     2281 cgtcggccag cgattcgttg tatctcacgg tctggcgggc caccacggtg cccaccacca
     2341 cggaaacctg gatgaaattg cgggggcgcg gcagccggcc gcgcagaccg aggttcacgc
     2401 ccacccggac atccgggtcg aaggtgtcgc gcagcgtggc caccgggagg aactccacca
     2461 ttccgccgtc gacgtacatg tcgtcaccga tctccacggg cgcaaagagg tgcggaatgg
     2521 tgctggagac gcgggccgcc aatgccacgc ttccctcgcg gaagatggcg cgatctcccg
     2581 tgaggaggtt ggtgccgaca atggccagtg gaatccggag ctgctcgaag gtccggcttc
     2641 cgaggaggtc ggtaaggaag cgatggacgg tatcgttcga gaggaaaccg agccgcggga
     2701 acgagagccg ggccagcttg ttccagcgga caccccgggc cagcgccttg agctccggga
     2761 tcgagaaccc ggcggcatag agcgcaccga tgatggcccc gcccgacgta ccggtgatgc
     2821 aggaaacccg gattccagcg tcctcgagtg cctcgaggac gccgatgtgg gcgattgtct
     2881 tggcggtgcc gccgctcagc gcgacgccga ccgtgacggg tgcggtcaca cctcgggcgc
     2941 gaaattggtg gtcgattcgc tgcgcacttc gaggacaagg gcgggactct ccccctggca
     3001 gatgtacgag cgccccgagc gcccgtcgag tcgggccgag tcgccgctct tcaaggccat
     3061 ggtctgggat tccacttcga tctcgatctg accctgctgg acgatgagaa agagctcgct
     3121 ggccgagtcg tgaccgggcc ggcgacagct ctggccgggg ttcaacccca cctgcagcgc
     3181 ggtcatcgtg cgggaaccgg ccagcacctc gaggatctcg gggctggtcg ggctggtgag
     3241 gcgcgtgcga tcgccccttc ggacgaccat cacgtcgcgc tgtcccggct gatcgaagaa
     3301 atagaccatc gcgattccca tcgcctcgga gatacgtcgc agggtgccga tcgatggggc
     3361 cgacaggcct cgctcgatcg tggaaatctg accgttcgtg acgccgacct gcctggcaag
     3421 gtccatctgc gtcaactgga gatcgcggcg aaaacgccgg atcctctcgc cgagtgcttt
     3481 ctgatactcc aggatgtcga actcggtagt ggtcttgtcc atgcacgctc cgcgtgtgcg
     3541 gcggtcgcaa catccggaac ggcctgaaag cggcaggcat acaggcgcga ccgggttccg
     3601 gttgcaacgg aaccgccaga tctcgggctt tgggcgccgt gaaattagca acccccacgc
     3661 cggagtgtca attccatcga gtggggcagc aaggacttgg acgattccga tcggaccgat
     3721 cgttccccgc caggtcggtc cgcctgctag gatccccggc catgaatcga atcgggatcc
     3781 tgaccagcgg cggggacgct cccggcatga acgcggcgat ccgggcggcc gttcggacgg
     3841 cgtcggtgcg gggagtggag atggtcggca tcaccgaggg ttacaccggc ctcctggccg
     3901 gcctcgttca cacgctgggg ccccgcgctg tctcaaacac catccagcgc gggggcacga
     3961 tcctcggcac ctcgcgctgc gaggagttcc ggaatccgga gggtcgccgc ctcggcgccg
     4021 aacgcctgcg tcacgccggc atcgatggac tcatcgtcat cggtggcgac ggctccttcc
     4081 gaggcgccga tcggctggcc acggaatgcg gcatcgcggt gatcggggta ccgggcacga
     4141 tcgacaatga catcagcggc accgacgcca cgatcggcta cgataccgcc gtcaacacgg
     4201 ccatcgatgc catcgacaag atccgcgata ccgccgcttc ccactcgcgg atcttcttca
     4261 tcgaggtgat gggccggcac gcaggtttca tcgcgctcga ggccgcgatc ggcggcggcg
     4321 cggaggaagt gatcctgccc gaggaagagg tcaccatcga actggtctgc gagcgcctcc
     4381 gcgacgggat gacgcacggg aagtcgagca gcatcgtcat cgtggccgag ggacatcccc
     4441 tcgggggggc ggtggcgatc gcggagcgcg tggaggccat cctgccggtg gataccaaga
     4501 ccaccatcct cgggcatatc cagcggggtg gccgacccac cgccagggac cgcgtcctgg
     4561 ccagccggct gggtgccgcg gcggtcgacc ggctcctggc cagcgagacg ggcgtcatgg
     4621 tcggcgaagt ggccggatcg gtgatgacga cgccgctccc ggatacctgg aaccagagga
     4681 agccactcga cgccgggctc ctccgactcg cccgcgaact ctccgcatga tccgaacgcc
     4741 gccgcaccgt atcggactcg tgctgctggc cgcgctgctc gccgtaacgg tcagcacgat
     4801 gtccgcttcg ggagccggga cggcccgggt caccttcgac cacctcacct gggcgccgga
     4861 aggaagtgcg ctggcggttg aagcacgctt cgagtcgtcc gacggcgtgg caaccgacac
     4921 gctgctcgtc gatctgaccg aggggagcgt ggccacacgc cagttccgac cgtcgcactt
     4981 cgcgctcgat gccggcggac gacgcatcgt ggcggcgggg cgctacgcga tggtcaccgc
     5041 gtcactcgat gaccccgacc ggctggagcg actttccggc cgtgacccgg cccgccagga
     5101 agtggtgcgg ctctccttca ccgcccgcgg agattcggtg ctcctcctct cccgcacgcg
     5161 gggatccgtc cgctatgaac tctcggcctg gccctggccc accggtcggc aggccatgcg
     5221 cctgatcact tccagccgga ccgacgccat ccgggcgttc aatgcggccg catccgcgac
     5281 gcgcccgctg gccgtcgagg aggaccctct cccccacctc taccggcaga tcggttcgaa
     5341 cctgtttcat ctcgaggcca gtcagacgcc ggtgcccggt gtcccgagct tcgggctctt
     5401 caacctcttc catcgggaca tggagagggg ccagtcgaaa ctccttgcca cgaacgtggc
     5461 cccgtacgat ctcctcgctt cccccgactc ggcctgggta gccgtcgcgg ggcaccgcgc
     5521 ggtggccgac tatggcgggc gcctggtgaa gtgcctctgg atcgcctcca cggacggcgc
     5581 gatcttcttc agcgtggcgc ccaccgggga gcgctcctat cacccggccg atgtgcgcgc
     5641 catgtgctgg gtgtcgcgga agctctggta ctcgactccc gatgggctct tcagtgtcga
     5701 ccccgccacc ggccgggccg tgcgcatcac gctcggaccg ccaccgccgg tctggagtgc
     5761 ccagctcgct ccatccactc ccctctggac cctcctctcc cgatcaagcg ccatcgacac
     5821 cgcggaagcg gtgcgggagc gggagcgact gcggaaggac ggctggcccg cctgggtgac
     5881 cacctggcag ggcgatcgct tccgggccgc gatcggatcg gccgtggacg aggcttcgct
     5941 ggcgcccctc tcgaagcggc tggtcgaagc cggccacacc ggcttctcgg ccgcacgggt
     6001 cgacccagtg gcctccggct cccccttccc atggggaatg gtgaagagcc cggtcgacag
     6061 ccgcgaggcc tacctggtgt ccgtcggtcc gcccggcttc accgccggcg aaatctggtt
     6121 gaccgagctc cagggcgccc gccagatccg cctggtcaag gccatgctgg tcggtcacga
     6181 cctgccgccc ggctccggct acgtgcctga gccgcccgag ccagggccgc gttgacttgc
     6241 ccttcggaac ttcttaagtt ctcacggttt tgatgccgcg atccgaaatg cgaggctccc
     6301 gcccatcctc cggggcgtcg tcgcacgacc agaaccctct ccccaaggat tcgatgcccc
     6361 gccggaccgc cccgctgccg ccgttgctgc gcctccttgt cgcgctcgtg atcgtcggca
     6421 ccccgatctt cgccgcgatc gtcgatggcg ccccgaggaa atccccacgg cgccggagct
     6481 tcacgcccga ccagatgcaa cgcctccgtg cggcggatga cgggctgcgg atcctcaccc
     6541 gcaatgcgcg attgagcgag acctacatct cgccgaaggg tgacggcgtg caggatgtca
     6601 cgctgatttc gtacgaagtc gtcgccccgg acagcatcac catgttcgtc tacctcgaca
     6661 ccgccgcctg gggaacggtc gaacacccgt ttcgcggaat catccgcgac tatgaactcc
     6721 ggccgccggg ccccaccgtc attccgttca atggcaacat cggcaccggt ccggatgcgg
     6781 cccggctgga cggggtgtac ggcgtgatca ttcgggccat cggcgatacc ggccgggtcg
     6841 aggtcgacac cttgatgctc gtgatcgacc gggtgagtcc cgtcttgcag tcggtgacca
     6901 ccctgggagg cctgtcgagc tatcggaacg gcgagacggt cgtgatccag atccaggcgg
     6961 accaacccgg ttatacggca tcggccaact tcagcgggat cgacagcgac ccgatcaacc
     7021 gtaccgaggt gctcgaactc ggaaatggcc gctacgagat ccgtcacgtg atttccgaca
     7081 ccaacacgaa ggcggacacg tccaacgtcc tcgtcccggt ccggctggtc gacctcgccg
     7141 ggaacttcac caactacaac gcgctccgcc tttgcctctc gaatcatcct cccgttctcc
     7201 tctccgcggt caccctgaac gcgccggaag gcgcctacaa gggcgaatcg ctcatccaga
     7261 tcgagaccac ctggcagagc gtggataccg cgctgacggt ccgggccgat ttcaagggaa
     7321 tcgacacggc ctacgattcc acgcggtacg tcacgtcccg gcttcccggc aatcgctatc
     7381 actcgaccta tcgtctgagc gacgccaacc agaagcccga cggcagctac ctggtgccga
     7441 tcgtggccag cgatcacggc tgcggcgtgt caccggcggc cctcgtcacc attgcactcg
     7501 acaccgaggc accgccgctg ccggtcttcg atgccgttcc caccggtgtg cgggagccgg
     7561 cttacacgct tcgcggtacg gccaacggtt ccgttcgtgt cctggtcctc cagaatgagg
     7621 cgctggtcga cacgttccag gtcaacccgg acgggcgatt cgaggtgccc gtcaccctcg
     7681 tgccgggcaa caatcgtttc acggctgagg gactcgacac ggccggcaac aagaccgtcc
     7741 gttcggtgcc ggtccagatc gtctacgtca ccggcgggta cgttacgatc ccgctcccct
     7801 tccgacccgg gaacaccttc caggtgggtg ccagccagcc ggccagtggc gtgcgcatcg
     7861 agctgtggac cttggggggc gatctcgcgc gcgtgcttac caacgagtcg ccggccgagg
     7921 tctacaacat ctcctgggat ggccgcgacg aggccggtga gcggctgaac tcggggcccc
     7981 tgatcgcgat catcaccacc gatttcaccg acggctcgag cgccaccgag aaacgcgcca
     8041 tggtccttgc ctccggggcg gggacgccat gatgcacctc aatcaacgac tcgggtcccg
     8101 cttgtggccg ctggtcgcat tcgccgccct cggcctcctc ctctcaccga agggcgttcg
     8161 tgcgggccac accaccacgg ccttcgccga catcgaaccg ggtccgcgga tcatggccat
     8221 gggcggcgcc ggcgccggcc tcgccaacga cccgaccgcg gcctggtgga acccggccgg
     8281 gctctacttc atgcgcggca cccagggcgt cgtcacctac gatgacctgt acggcgaggg
     8341 gctgatccag cgcaattacc tgagcgtggc ctggaaaaag gttcattacg aaccccgctt
     8401 cgacgacaac gtcctcacgc tcgagcgcga cctgaaccgt ggatcggcat tcggcttctc
     8461 ggtttcttcg ctcttcctcg acctggacga gtccagctac tcggagttca tgcccacgct
     8521 gtcgtacgcg gcgggcgtgg gcggaggcgt cggcctcggg gcgtcgctca gtttcctgaa
     8581 ggccagcagt gacgtggtcg gggtcggcag caccggctac tcggccacgg tcggcgtttc
     8641 cttcccgctt ccgtgggatg cccggttcgg ctacgccgcg cgcaacctct tttcgagcct
     8701 gagccccgag gagggggccg gcgaaaagct gccgatcacc agcgccgtgg gagtttccgc
     8761 gccgatcggc aaggcggggc tcgtggccgc cgacgccacg ttctccgacg gcgattcacg
     8821 cttcggggcc ggcgccgagt actggttctt caaccatcac ctggccgggc gggcgggcct
     8881 ccggcaattc gacggcgccg tcgagtcgcg cgtcgtgccg ac
//
LOCUS       DASVOD010000012         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_365913, whole genome shotgun sequence.
ACCESSION   DASVOD010000012 DASVOD010000000
VERSION     DASVOD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365913"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VF720_00230"
     CDS             complement(<1..403)
                     /locus_tag="VF720_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877802.1"
                     /translation="MTPSFRKGVTVLRRHRHSSLIVNLLALGTLVMAPSAAAQTGLVP
                     LTDLGSGTHGGYPGGLYPGSLNTPPPAHLLDALTLADGVVPRDAAGNPSTDGWIGMIA
                     VGMSNTTHEFAVFERNADADPSRNARVVILDT"
     gene            complement(409..1476)
                     /locus_tag="VF720_00235"
     CDS             complement(409..1476)
                     /locus_tag="VF720_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7877803.1"
                     /translation="MLAWILFLALILAFLALDLGVFHRKSHAVGVREALAWSGVWIVL
                     ALGFSFVVRLAYDAGWQGLGAHPDPVDGTRLTGSTAMVKFLTGYIVEKSLSIDNVFVI
                     ALIFGSFGVPPRYQHRVLFWGILGALAMRGVMIGLGAVLVARFHWVLLIFGGFLVLTG
                     VRMLFLKDAHGDPSQSRVLRWARRFFPVTDRFHGQHFIVKAGSAASAEPDEPGQAVAP
                     DAGVSRARPGSWMITPLALALMLVESADLLFAVDSIPAIFAVTGDPFLVFSSNVFAIL
                     GLRSLYFALAGAIDRFRHLKTSLALVLLLVGIKMLAAEPLKHWLGAAFNLYLLAGVLL
                     ILAVGVIASWRRPAGPVPGHG"
     gene            complement(1485..1799)
                     /locus_tag="VF720_00240"
     CDS             complement(1485..1799)
                     /locus_tag="VF720_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX7877804.1"
                     /translation="MNCPRCDVVVLDEKTRDGVVIDACPRCRGIWLDRGELEKLIARS
                     LHDEEGRGTYGDDRRPGERRDDDRRRDDDDRGWRGRDDDDDDDRNRRRGGFLGSLGDL
                     FD"
     gene            1926..>2124
                     /locus_tag="VF720_00245"
     CDS             1926..>2124
                     /locus_tag="VF720_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7877805.1"
                     /translation="MDWLNYHHLLYFWTVARHGTIVRAARELNLSHPTISSQIRSLEE
                     SLGERLFTRIGRRLELTDVGRT"
BASE COUNT          410 a          708 c          703 g          303 t
ORIGIN      
        1 ccgtatcgag aatgacgacg cgggcattac gggagggatc ggcatccgcg ttgcgctcga
       61 acaccgcgaa ttcatgcgtc gtgttcgaca tgccgacggc gatcatgccg atccagccgt
      121 ccgtgctcgg attgccggcc gcgtcgcgcg gaaccacgcc gtcggcgagc gtcagggcat
      181 ccaaaaggtg cgcggggggc ggcgtgttca ggcttcccgg gtagaggccg ccgggatagc
      241 cgccatgcgt tccggagccg aggtcggtca gcggaacgag ccccgtctgg gccgccgcgg
      301 aaggggccat gaccaacgtg ccaagggcaa ggaggttcac gatcaacgat gaatggcgat
      361 ggcggcgcag cacggtcacc cccttccgaa acgaaggggt caatcatatc agccgtggcc
      421 aggcaccggt cctgcgggac gccgccacga cgcaatcacc cccaccgcca ggatcaggag
      481 cacgcccgcc aggagataga ggttgaacgc cgccccgagc cagtgcttca ggggttccgc
      541 cgcgagcatc ttgattccca ccagcaggag gacgagcgca agcgacgtct tgaggtggcg
      601 gaaccggtcg atggcgcccg ccagcgcaaa gtaaagcgat cgcaaaccca gtatcgcgaa
      661 gacattgctg ctgaatacga ggaatggatc gcccgtgacg gcaaagatgg ccgggatgga
      721 gtcgaccgcg aagaggaggt cggcgctctc caccagcatg agcgccaggg ccaatggcgt
      781 gatcatccag gaaccggggc gggcgcgcga cacgcccgcg tccggcgcaa ccgcctggcc
      841 cggctcgtcc ggctccgcgg aggccgcgct gccggctttc acgatgaagt gctggccgtg
      901 gaagcgatcg gtaacgggga agaaccgtcg ggcccaccga agcacgcggc tctgcgaggg
      961 atcgccatgc gcgtctttca ggaagagcat ccggacgccg gtcaacacga gaaatccgcc
     1021 gaagatgagc aacacccagt ggaaccgcgc caccagcact gctcccaacc cgatcatcac
     1081 cccacgcatg gccagcgcac cgaggatccc ccagaagagc acccggtgct ggtagcgggg
     1141 cggcactccg aacgatccga agatcagcgc gatgacgaag acgttgtcga tgctgagcga
     1201 cttctcgacg atatagccgg tcaggaactt gaccatggcc gtcgatccgg tgagccgcgt
     1261 gccatccacc gggtcgggat gcgcgcccag tccctgccag ccggcatcgt acgccagccg
     1321 caccacgaac gaaaaaccga gcgccaggac gatccacacc cccgaccacg ccagcgcttc
     1381 gcggaccccc acggcatgcg acttccggtg gaacacgccg aggtccagcg cgaggaaggc
     1441 gaggatcagg gcaaggaaga ggatccaggc gagcatggag tcgatcagtc gaacaggtcg
     1501 ccgagggatc cgaggaaacc gccccgccgg cggttccggt cgtcatcgtc atcgtcgtcc
     1561 cggccgcgcc agccgcgatc gtcgtcgtcg cggcggcggt cgtcgtcgcg gcgttcaccc
     1621 gggcgccggt cgtccccgta tgtcccgcgg ccctcctcgt cgtgcaacga ccgggcgatc
     1681 agcttctcca gttcgccgcg gtccagccag atgccgcggc agcgggggca ggcatcgatg
     1741 acgacgccgt cccgggtctt ttcgtcgagc acgacgacgt cacagcgggg gcagttcatg
     1801 tggggcacct cgtggccatg gtcaggacgg cttgcgaacg tcccgatcat ggccgtcttt
     1861 cgtcatgtag actatttgat aatttggatg caatctgtcg atatatccaa caggggggcc
     1921 ccggcatgga ctggctgaat taccaccacc tcctctactt ctggaccgtg gcgcgccacg
     1981 gcacgatcgt gcgcgcggcg cgcgagctga atctctccca tcccaccatc agctcgcaga
     2041 tccgctccct ggaggaatcg ctgggggaac ggctcttcac ccggatcggg cggcggctcg
     2101 aactcaccga cgtggggcgc accg
//
LOCUS       DASVOD010000013         4935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_731275, whole genome shotgun sequence.
ACCESSION   DASVOD010000013 DASVOD010000000
VERSION     DASVOD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731275"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="VF720_00250"
     CDS             <1..1520
                     /locus_tag="VF720_00250"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEX7877806.1"
                     /translation="YAHNNFLNWRVPALWFSPEVFGDVASLDMPIAIFGRPYMSGTGW
                     GAIGVVPLGLVFFALVSRVGPVRERLFWAGLGGITLAFLFFLATPAYHVLIRFLPWLD
                     DVDLLRGLLVPTLCGALLAGWGAAELVAAWSRGTPRSQWLLPWLGFIAIGVVAILVGR
                     SGLSMDRSLPGDLAPLLGLCGGVLLIGLLPFSCANWQRARTCLLLALVVGELGWLHYK
                     VNTFASPAHTFPEHPMVTDLKARLGPPDYSRYLVLGRFRFLPPNTGATYELADIRGYS
                     PIPIARFRTLIECAEGRSGLENSAFTENFESPIYRLLGASHVVLGSKEPPPGYEKVWN
                     RYLYRNPASLPRAFLVHDVVSIDGEAAMRAALGAANFDPGTRAYLERTAMPATSLHPP
                     TGTESVRITAMEPARVVIEAEASSPALLVLSDPYYPGWRATVDGIDTRILPADWALRG
                     VEVPPGRHRVEFRYRPAWLIPGFIAMVVGTVLAVIGLAFTGSPSASPRHPTGRRT"
     gene            complement(1468..4401)
                     /locus_tag="VF720_00255"
     CDS             complement(1468..4401)
                     /locus_tag="VF720_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7877807.1"
                     /translation="MSRTLRPGDDISRYRIVGPLGAGGMGEVYRARDTNLDRDVALKV
                     LPPTLVRSEERVRRFVLEAKSASSLNHPNIVTIYEIGEDQVSSSDGTPVDPPSKAHFI
                     SMELVSGDTLAAKIHDEKTDLKTLLGWMAQAADGVAKAHAAGIVHRDLKPGNIMVSND
                     GYAKVLDFGLAKLTEKVDLDTVLSTAPTEIGNRTTEGVVLGTVGYMSPEQVRGKGVDA
                     RTDIFALGCILYECATRRRPFVAESSIETMHAILNGTPTPIEEFNPGVPAELRRLIRR
                     CLSKSTDQRLQSAKDLAIELREIVDDYENLSASATSAGSGGTAAPAMPSTGGSGATMA
                     PPSTGSGSVAAPPRRLPWIPILVTALVAAGVAVGSSFFRKSAKPVAAPADIKVTTITN
                     KGDVSNPVISPDGRYLAYVSGPIDKRQVYVRQIATGSDIEVLQSLPVPPERLNFSPDG
                     NYLYYLAPDPEHPGYRGLYRVPSLGGASTRLIYDVDSKPTFDPTGTKLAFIRGVPQEK
                     AYDLVILDIASGKETRLKRTVDPENISPSPEWSPDGEWIACSYQPGDKSGATRLGLVR
                     VKDGSIRIVPKFQVYDTRSASWAWLPDGRSIVSTGYTLDEANPYPVRLVSITDGSSHR
                     LTRTMDSWSDVSVSADGNQLVAQRRVTTSNLWVMDVDVPGSLPKQLTFTTGGEQAVGD
                     FQGIESGDIVYMQGGSDAVSLSAMKADGTGIRSLRSDNANDFFLRLRPNGWFVQRLIR
                     QDNGEADLSTWRYDGESGARRKIGHSTSELLMDVSRDGKHLLVCDWHDPSRFFHMGPD
                     GGDFRQVADDASGPDASFSTDGTRFLYRAFRTVNGENQLRYTVRNIADGTMIPVPELP
                     PGADDVTWSADGRSLYYFDWLSGGMSRQGLDGGAPVPIGRFAPGRVTELSLSPDGTRL
                     VARVVDGAVQNLWMMDADGKNAVQLTHFLTGDLFNAEWSTDGKRVIFTYGDRSDDVVM
                     LTGFR"
     gene            complement(4415..>4935)
                     /locus_tag="VF720_00260"
     CDS             complement(4415..>4935)
                     /locus_tag="VF720_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX7877808.1"
                     /translation="TPFFIGDGRRDELLLEAGIARARSIILATDDDLAHMEMALDARR
                     LNPKIRVVLRMFDQALADKIKEGFDIHLAMSQSAISAPAFATAALDGTMVGSFVVGDE
                     LIVLQRRTVDGAWNGKRIDEIGKAVGGVVVEQVTGGARRLFPSPEKAVALGDELLLQA
                     PFENLRVQNGRH"
BASE COUNT          719 a         1614 c         1710 g          892 t
ORIGIN      
        1 actacgcgca caacaacttc ctcaactggc gcgtgccggc actctggttc agccccgagg
       61 tcttcggcga cgtggccagc ctcgacatgc cgatcgcgat tttcggccgc ccgtacatga
      121 gcggcacggg gtggggcgcg atcggcgtgg taccgcttgg cctcgtcttc ttcgcgctgg
      181 tgagtcgtgt cggaccggtg cgggagcgac tgttctgggc cggtctcggg ggcatcaccc
      241 tggccttcct ctttttcctg gcgactcccg cttatcacgt actgatccgg tttctcccgt
      301 ggctggacga cgtggacctg ctccggggac tgctcgttcc gacgctgtgc ggggcactgc
      361 tggccggctg gggcgccgcg gaacttgtcg ccgcctggtc gcgcggcaca ccccgatcgc
      421 agtggctcct cccgtggctc ggcttcatcg ccatcggcgt cgtggccatc ctggtgggcc
      481 gaagcggtct gtcgatggac cgttcgctcc ctggcgacct ggcgcccctg ctcggcttgt
      541 gcggcggtgt actcctcatc ggcctcctgc cgttcagctg cgcgaactgg cagcgggcgc
      601 gcacctgcct cctcctggca ctcgtggtcg gcgagctggg ctggcttcac tacaaggtga
      661 acacgttcgc ttcgccggcc cacacgttcc cggaacaccc gatggtcacc gacctcaagg
      721 cccggctcgg tccgcccgac tattcccgct atctcgtgct gggccgtttc cgattccttc
      781 ccccgaacac cggcgccacc tatgaacttg ccgacatccg cggctattcg ccgattccca
      841 tcgcgcgctt ccggacgctc atcgagtgcg ccgaaggacg atccggcctg gagaattcgg
      901 ccttcaccga gaatttcgag tcgccgatct atcggctgct gggggcctcg cacgtcgtgc
      961 tgggttcgaa ggaaccaccg ccgggatacg agaaggtctg gaatcgatat ctctatcgga
     1021 atcccgcgtc gttgccgcgg gcctttctgg tgcatgacgt cgtctcgatc gacggcgagg
     1081 cggcgatgcg cgccgcgttg ggggccgcaa acttcgatcc tgggacacgc gcctacctgg
     1141 aacgaaccgc gatgcccgcg acatcgctcc atcctccgac ggggacggag agtgttcgca
     1201 tcaccgcgat ggagcccgcg cgtgtcgtca tcgaggcgga ggcgtcgtca cccgcgctcc
     1261 tcgtcctgag cgatccgtac tatcccggtt ggcgggccac ggtggacggt atcgacacac
     1321 ggatcctgcc ggcggactgg gccctgaggg gcgtcgaggt tccgcccggg cgacatcgcg
     1381 tggagttccg gtaccgaccg gcgtggctga tcccggggtt catcgcgatg gtggtcggga
     1441 cggtgctggc ggtgatcggc ctggcgttta ccggaagccc gtcagcatca ccacgtcatc
     1501 cgaccggtcg ccgtacgtga agatgacgcg tttgccgtcg gtgctccact cggcgttgaa
     1561 gaggtcgccg gtgaggaagt gggtgagctg gacggcgttt ttgccgtcgg catccatcat
     1621 ccagaggttc tggacggcgc catccacgac gcgggccacg aggcgcgtgc cgtcggggga
     1681 gagcgacagc tcggtgacac gcccgggcgc aaagcggccg atcggcacgg gtgcaccgcc
     1741 gtccagcccc tggcgcgaca tcccgccgga aagccagtcg aagtagtaga gcgaccggcc
     1801 gtcggcgctc caggtgacgt catcggcgcc cggcggcagt tccggcacgg ggatcatcgt
     1861 accgtccgca atgttccgga cggtgtagcg cagctggttt tcgccgttga cggtccggaa
     1921 ggcccgatag aggaagcgcg tgccgtcggt cgagaagctc gcgtcggggc ccgacgcgtc
     1981 gtcggccacc tggcggaagt cgccgccgtc cggtcccatg tggaagaatc ggctcggatc
     2041 gtgccagtcg cagacgagga ggtgcttgcc gtcgcgcgac acatccatga gcaactcgct
     2101 ggtcgagtgg ccgatcttcc ggcgggcgcc cgattcgccg tcgtaccgcc aggtgctcag
     2161 atcggcttcg ccattgtcct ggcggatgag gcgctgcacg aaccagccgt tgggacggag
     2221 tcggaggaag aagtcgttgg cgttgtccga gcggagactg cggatacccg tgccgtcggc
     2281 cttcatggcc gacagggaga cggcatcgct gcctccctgc atgtagacga tgtcgcccga
     2341 ttcgatcccc tggaagtcgc cgacggcctg ctcgccgcct gtcgtgaagg tcagctgctt
     2401 cggaagtgaa ccggggacgt cgacgtccat cacccagagg ttggaggtgg tgacccgccg
     2461 ctgcgccacc agctggttgc cgtcagccga cacgctcacg tcggaccacg aatccatcgt
     2521 ccgggtgagc ctgtggctgc tcccatccgt gatcgacacg agccggacgg gatagggatt
     2581 ggcttcgtcg agcgtgtagc ccgttgaaac gatgctgcgg ccgtccggca gccaggccca
     2641 cgacgcgctc cgcgtgtcgt agacctggaa tttcggcacg atccgtatgg aaccgtcctt
     2701 gacgcggacg agccccaggc gggtggcgcc cgatttgtcg cccggctggt acgagcaggc
     2761 gatccactca ccgtcgggac tccactccgg tgagggtgag atgttttccg gatcgaccgt
     2821 gcgcttgagg cgcgtctcct tgccggacgc gatgtcgagg atcacgaggt cgtaggcctt
     2881 ttcttgtggc acaccccgga tgaacgccag ctttgtcccc gtcgggtcga aggtcggctt
     2941 gctgtccacg tcgtagatga gccgcgttga ggccccgccg agcgacggaa cacgatagag
     3001 cccgcgatag ccggggtgct cgggatcggg ggccaggtag tagaggtagt tgccgtccgg
     3061 cgagaagttg agccgctccg gcggcacggg aagcgattgc agcacctcga tgtcgctgcc
     3121 ggtggcgatc tggcggacgt acacctgtcg cttgtcgatc ggccccgaca cgtacgccag
     3181 gtagcggccg tccggggaga tgaccggatt ggagacgtcg cccttgttgg tgatcgtggt
     3241 caccttgatg tcggcgggcg ccgcgaccgg cttcgccgac ttgcggaaga acgacgaccc
     3301 gacggccacg cctgccgcga cgagcgcggt gacgagaatg ggaatccagg ggagtcgtcg
     3361 cggcggagcc gcaaccgaac cggagcccgt ggacggtggc gccatcgtgg cgccggaccc
     3421 gcccgtcgac ggcatcgcag gcgccgccgt cccgccgctc cccgccgagg tggcgctggc
     3481 cgacaggttt tcgtaatcgt ccacgatctc gcgcagctcg atggccaggt ccttggccga
     3541 ctggagccgc tggtcggtgc tcttcgacag gcagcggcgg atgagccggc gaagctcggc
     3601 gggcacgccg gggttgaact cctcgatggg cgtgggcgtg ccgttcagga tggcgtgcat
     3661 cgtctcgatg ctcgattcgg ccacgaaggg gcggcggcgt gtggcgcact cgtagaggat
     3721 gcagccgagc gcgaagatgt ccgtccgcgc gtccactccc ttgccgcgca cctgttcggg
     3781 tgacatgtac ccgacggtgc cgagcaccac gccttcggtc gtacggttgc cgatctcggt
     3841 gggggcggtc gagagcacgg tgtcgaggtc gaccttctcg gtgagcttgg ccaggccgaa
     3901 gtcgagcacc ttcgcgtagc cgtcgttgga aaccatgatg ttgccgggct tgagatcccg
     3961 gtggacgatg ccggccgcgt gggccttggc cacgccgtcg gccgcctgcg ccatccagcc
     4021 gaggagcgtc ttgaggtcgg tcttctcgtc gtggatcttg gcggccagcg tgtcgcccga
     4081 caccagctcc atcgagatga agtgggcctt cgacgggggg tcgaccgggg tgccgtcgct
     4141 ggacgacacc tggtcctcgc cgatctcgta gatcgtgacg atgttcgggt gattcagcga
     4201 gctggccgac ttggcctcga ggacgaagcg tcgcacgcgc tcctcgcttc gcacgagtgt
     4261 gggcggcagg accttgaggg ccacgtcacg gtcgaggttc gtatcgcggg cacggtacac
     4321 ctcgcccatc ccgccggcgc cgagcgggcc gacgatgcga tagcgggaga tgtcgtcgcc
     4381 ggggcggagg gttcggctca tgccgacatt ctactcaatg acgtccattc tggactctca
     4441 ggttttcgaa gggcgcctgg agcaggagct cgtcccccag ggccacggcc ttctcgggcg
     4501 atgggaagag gcgtcgcgcg ccgcccgtca cctgctcgac caccacgcca ccgacggctt
     4561 ttccgatctc gtcgatccgc ttgccgttcc acgctccgtc caccgtgcgg cgctgcagca
     4621 cgatcagttc gtcgcccacc acgaagctgc cgaccatcgt gccgtcgagc gccgccgtcg
     4681 cgaaggccgg cgccgagatc gcggattgcg acatcgccag gtggatgtcg aacccctcct
     4741 tgatcttgtc ggccagcgcc tggtcgaaca tgcgaaggac gacgcggatc ttcggattca
     4801 gccgacgcgc gtcgagcgcc atctccatgt gggccaggtc gtcgtcggtg gccaggatga
     4861 tgctgcgggc acgggcgatg ccggcttcga ggagcagctc atcgcgccgg ccgtcgccga
     4921 tgaagaaggg ggtgc
//
LOCUS       DASVOD010000014         2636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_157376, whole genome shotgun sequence.
ACCESSION   DASVOD010000014 DASVOD010000000
VERSION     DASVOD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157376"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF720_00265"
     CDS             <1..365
                     /locus_tag="VF720_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7877809.1"
                     /translation="RGSFPTLVFLSLLFILVMLGFGLFMSTLAKNQQQAMMGSIFLIM
                     VPMMYLSGLVFPIENMPKPIQLVTWVIPLRYYTNVLRGVFLKGSGMAVLWPEALALAV
                     SGVVVLTLASLRFRKRLD"
     gene            complement(435..1580)
                     /locus_tag="VF720_00270"
     CDS             complement(435..1580)
                     /locus_tag="VF720_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7877810.1"
                     /translation="MVKDKNEISVDKLREWLEKGKAVSVLDVRPEAERAEWSIPGSFH
                     VDAYRKLWENDPDALAGVDLTKDDPVVTVCAKGRTSLLARERLEAAGYKAYSLVGGMK
                     AWSLAWNVAEVPIPPAKGDVPGGPSEVFVLQFRRTGKGCLSYLVAAGGRAVVIDPSLD
                     PKVYIEAAAKRGWTIAASLDTHVHADHLSRARLIEKLTGAPVALPETTRVSFPYQVVR
                     DSDALPLSASVTLSAMRTPGHTPESICWRLDNRAIFTGDTLFIDAVGRPDLAASAKGA
                     EQRAHALWRSLEHLFTLPSTLLVLPTHTDHPVPFDKTPIMATLGEVQEAVSGLPKSEK
                     EFVSTLLSRIPPPPPMYDEIVKLNEKGTMPPGDVTDFEAGANRCAIK"
     gene            complement(1722..>2636)
                     /locus_tag="VF720_00275"
     CDS             complement(1722..>2636)
                     /locus_tag="VF720_00275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7877811.1"
                     /translation="GLGAGTRLMQAALREAREAGYPLSGLYPATQPIYRSVGYEQAGT
                     RWKTSVDAAKIDVKDHALLVSPITDADHPAIREVYAAKARRHEGHLDRNEYIWKRVFK
                     PKGEPPRGMKVVGDSGIEGYVVLTKVPREGSKADFSCLDVATLTPAAGRRILSLFADH
                     KSLADRFTWYGGPDDPLLYLLREQEPEMEAQTFWMLRLLDVPKALELRGYAPGRAGEL
                     HLRVEDPLFPENDGPWVLSVEGGRGHVVPGGRGSFRVSIRGLAPLYSGFLRAGVLAAI
                     GWVNAPSDELATAASLFDGPAPWTPDMY"
BASE COUNT          404 a          896 c          889 g          447 t
ORIGIN      
        1 tgcggggttc gttcccgacg ctggtcttcc tgtcgctgct cttcatcctg gtgatgctcg
       61 gcttcggcct cttcatgtcg accctggcga agaaccagca gcaggccatg atgggatcga
      121 tcttcctcat catggtgccg atgatgtatc tctccgggct cgtcttcccc atcgagaaca
      181 tgccgaagcc gatccagctc gtgacgtggg tcatcccgct gcgctactac acgaacgtgc
      241 tgcgcggggt gttcctcaag ggatcgggga tggccgtgct ctggccggag gctcttgcac
      301 tggcggtttc gggcgtggtg gtgctgacgc tggcgagcct cagattccgg aagcggctgg
      361 actaagtgcg cgccgacagg cgcgcgggcc cttgcataca ccaaaggcat tcccggccgc
      421 cctgatgatc cgccctactt gatcgcacag cgattggccc cggcctcgaa gtcggtcaca
      481 tcgcccgggg gcatcgtccc cttttcgttg agcttcacga tctcgtcgta catggggggc
      541 ggcggcggaa ttcgcgagag gagcgtggac acgaactcct tttcgctctt cggcaggccg
      601 gaaaccgcct cctgtacctc ccccagcgtg gccatgatcg gcgtcttgtc gaatggcacc
      661 gggtgatccg tgtgcgtcgg caggacgaga agcgtggacg gcagcgtgaa gaggtgttcg
      721 aggctgcgcc acagcgcgtg cgcgcgctgt tcggcaccct ttgccgaggc ggccaggtcg
      781 ggacggccga cggcgtcgat gaagagcgtg tcgccggtga agatggcccg attatcgagc
      841 cgccagcaga tgctctcggg cgtgtgaccg ggcgtgcgca tggcggagag ggtcacggag
      901 gcggagagcg gcagggcatc gctgtcacgc acgacctggt agggaaagga cacgcgcgtg
      961 gtttccggca gcgccaccgg ggcgcccgtc agcttctcga tcaggcgcgc gcgggaaaga
     1021 tggtccgcat ggacatgcgt atcgaggctg gccgcgatcg tccagccccg cttggcggcg
     1081 gcctcgatgt acaccttggg atcgagcgac ggatcgatga cgaccgcgcg cccccccgcg
     1141 gcgacgaggt acgacaggca ccccttgccg gtccggcgga actggagcac gaagacttcg
     1201 ctcggcccgc cgggcacatc tcccttggcc gggggaatgg gcacctcggc cacgttccat
     1261 gccaggctcc acgccttcat gccgccgacc aacgaatacg ccttgtagcc ggccgcctcg
     1321 agccgctcgc gcgccaacag gctggtgcgc cccttcgcgc agaccgtcac caccgggtcg
     1381 tccttcgtga ggtccacgcc ggcgagggcg tccggatcgt tctcccacag cttgcgatag
     1441 gcatccacat gaaaactgcc gggaatggac cactcggccc gctcggcctc cggccggaca
     1501 tcgaggaccg acacggcctt gcccttctcc agccactcgc gcagcttgtc gacggagatt
     1561 tcgttcttgt ccttcaccat gtgggggagc gctcccgctg tgctgatttc cctggccaat
     1621 ttccccggcc gacggcgccg ggcgacgcag gatgatagcg caaatgtggg ccggggtcgg
     1681 ccgggggatg gcctcgccga agcgatcaac gtggagcgcc cctagtacat gtccggcgtc
     1741 cacggcgccg gcccgtcgaa cagggaggcc gccgtggcga gctcgtcgga tggcgcgttc
     1801 acccagccga tggccgccag caccccggcc cgcagaaacc ccgagtagag gggcgcgagg
     1861 ccccggatcg acactcggaa cgatccccgc ccgccgggga cgacgtgccc gcgccccccc
     1921 tcgacgctca ggacccaggg accgtcgttc tcggggaaga ggggatcctc caccctgaga
     1981 tgcagttcgc ccgcccgtcc cggcgcgtag ccgcgcagtt caagggcctt ggggacgtcg
     2041 agaagccgga gcatccagaa cgtctgcgcc tccatctcgg gttcctgttc gcgcaacagg
     2101 tacagcagcg gatcgtccgg gccgccgtac caggtgaacc ggtcggcgag gctcttgtga
     2161 tcggcgaaca ggctcaggat gcgacgaccg gcggccggcg tcagcgtggc cacgtccagg
     2221 caggagaagt cggccttgct cccctcccgc gggaccttgg tgagcaccac gtacccttcg
     2281 atgccggagt ctcccaccac tttcatcccg cggggcggct cccccttcgg cttgaagacg
     2341 cgcttccaga tgtactcgtt ccgatcgaga tgtccttcgt ggcggcgcgc cttcgcggcg
     2401 tatacctcgc gaatggcggg atggtcggcg tcggtgatcg gcgacaccag gagcgcgtga
     2461 tccttgacgt cgatcttcgc cgcgtcgacg gaggtcttcc agcgggttcc agcctgctcg
     2521 taaccgaccg agcggtagat gggctgcgtg gccgggtaga gccccgagag cggatagccg
     2581 gcctcccgcg cctcgcgcag cgccgcctgc atgagacgcg tgccggcccc cagccc
//
LOCUS       DASVOD010000015         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_470444, whole genome shotgun sequence.
ACCESSION   DASVOD010000015 DASVOD010000000
VERSION     DASVOD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470444"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..728)
                     /locus_tag="VF720_00280"
     CDS             complement(45..728)
                     /locus_tag="VF720_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877812.1"
                     /translation="MSEHIERPAKSDRDYLRRKLFSFFGLVPMALYVFLHLAAHSAAW
                     LGAHVWEERLRDWHGSPFYWPVVVAFIYVPFAFHTVWGIVLALRGRATGSGGLTTFGY
                     VKYVLQRLSAVGVLLFLGAHIYKTRIEPALHGEPLDFDHMVWAMHHVPTILVYALGVL
                     GVAFHMANGLWLAGITWGLTVSRPSQRAWQAWTIAAFVVIAAMGFVAMWGFARVPLDG
                     HAGQAMLGR"
     gene            914..1810
                     /locus_tag="VF720_00285"
     CDS             914..1810
                     /locus_tag="VF720_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002615987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7877813.1"
                     /translation="MIRLIARQGVLLLCVVAALTSFHGCGGGGGGNKADGGNRSGGGR
                     ARAITLKGSDTMVILGQKWAEVYMASHPGSVIQVTGGGSGTGIAALINGTTEIAQSSR
                     TMKDTEKGQAEEKSGRKVEEHTVALDGVTVYVNNGNTVGTLSTGQLKGIYTGQITNWK
                     EVGGSDMPIVVYGRESSSGTYVFFKEHVLGGADFAPQTQTLPGTAAVADAIGKDPKGI
                     GYGGYAYGVGIKHVSVSKEDGGTAVEPSEATVRDGSYPLSRPLYWYTLSSPNPAAVEF
                     LTWVMSAEGQKVVKDVGYFPIN"
     gene            complement(1853..2326)
                     /locus_tag="VF720_00290"
     CDS             complement(1853..2326)
                     /locus_tag="VF720_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7877814.1"
                     /translation="MTPQPVRSSHTGMLIRRPVVTVYEAFVDPAVTTRFWFTHGSGRL
                     DAGKPVEWRWAMYDVAVTVTPKAIEPGRRILIEWPGDPEPRTVEWLFESVGEDATFVR
                     ITENGFTASGAELVSQVADSTGGFTLALAGAKAFLEHGIELNLVADRFPKGLAGQ"
     gene            complement(2331..>2950)
                     /locus_tag="VF720_00295"
     CDS             complement(2331..>2950)
                     /locus_tag="VF720_00295"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX7877815.1"
                     /translation="DSGDPVALSKRILDGVGHTPVGNGNGNGHGDLLDTITDHKLVAP
                     DTGANPIQFVHVFPGTPDRKVHLVPPDLDAEAPGGLYHWQPDPGTALYPLALISPALG
                     TTVSSTFAQLIPGHVPVEIHPDDAAPRGIVNGEPVRIHNELGEVFTIARINPDVRPGV
                     VSLPKGLWARHTKNGLTSNALSPATHSDVGGNACFNDARVEIEKA"
BASE COUNT          537 a          890 c         1061 g          462 t
ORIGIN      
        1 tcctggggga gaactggttg aggatgctgg ggtaggccgg aggcctaacg ccccagcatc
       61 gcctgccccg cgtgtccatc cagaggcacg cgcgcgaatc cccacatcgc cacgaagccc
      121 atggcggcga tgacgacgaa ggcggcgatc gtccaggcct gccaggcgcg ctgcgagggg
      181 cggctcacgg tgaggcccca ggtgatgccg gccagccaga ggccgttggc catgtggaag
      241 gccacgccga ggacgccgag cgcgtagacc aggatcgtgg ggacgtggtg catggcccac
      301 accatgtggt cgaaatcgag cggctcgccg tggagggcgg gctcgatgcg cgtcttgtag
      361 atgtgcgcgc cgaggaagag gagtaccccg acggccgaga ggcgttgcag gacgtacttc
      421 acgtagccga acgtcgtgag gccgcccgag ccggtggcgc gtccgcgcag ggccaggacg
      481 atcccccaca ccgtgtggaa ggcgaacggc acgtagatga acgcgacgac caccggccag
      541 tagaacgggc tgccgtgcca atcccgcagg cgttcttccc agacatgggc tcccagccag
      601 gcggccgagt gcgccgcaag gtgcaggaag acgtagaggg ccatcgggac gagcccgaaa
      661 aacgagaaaa gcttgcggcg gaggtagtcc cggtcggatt tggcgggacg ttcgatgtgt
      721 tcggacatgg atctcccggg ggccggcgcg gcgtgcgcgg cggcaaattg gacagccgcg
      781 cacgataaca aaggggtttg ggggtgtcca gccgagggaa ataaaggtgg cggtcgccat
      841 cgaatcgcca caatctgttt cgtattgtga ttgctggacc tgtttgccat atcgttttca
      901 tacggaggaa gtcatgattc gattgatcgc ccgccagggc gtccttctgt tgtgtgtcgt
      961 ggccgcgctg acgtcgttcc atggctgcgg cggtggtggc ggtggaaaca aggccgatgg
     1021 aggaaatcga tccgggggtg gccgtgccag ggccatcacc ctcaagggat ccgacaccat
     1081 ggtgatcctt ggccagaagt gggccgaggt gtacatggcc tcacacccgg gcagcgtcat
     1141 ccaggtgacg ggcggcggct cgggaaccgg catcgccgca ctgatcaacg gcacgaccga
     1201 gattgcccag tcgtcgcgca cgatgaagga caccgagaag gggcaggccg aggagaagag
     1261 cggccgcaag gtggaagagc acaccgtggc gctggatggc gtgacggtct acgtgaacaa
     1321 cgggaacacc gtcggcacgc tcagcacggg ccagctcaaa ggaatctata ccggccagat
     1381 caccaactgg aaggaagtgg gcggcagcga catgccgatc gttgtgtacg gccgcgagag
     1441 ctcatccggg acgtacgtct tcttcaagga acacgtgctg ggtggcgccg acttcgcacc
     1501 gcagacgcag accctgccgg gcaccgccgc cgtggccgac gccatcggga aggaccccaa
     1561 ggggatcggc tacggcggtt acgcctacgg cgtggggatc aagcacgtct ccgtgagcaa
     1621 ggaagacggc ggcaccgccg tcgagccgag cgaggccacg gtgcgcgacg ggagctatcc
     1681 actgagccgg cccctctact ggtacaccct ctcgagcccg aacccggcgg ccgtggaatt
     1741 ccttacgtgg gtgatgtcgg cggaggggca gaaggtcgtg aaggatgtgg ggtactttcc
     1801 gatcaactag gcggcgggcg ctccataccg agacgaagct gtggaccgga cgtcactgcc
     1861 ccgcgagccc tttcggaaag cggtcggcca cgaggttgag ctcgattcca tgctcgagaa
     1921 acgccttggc cccggccagc gccagcgtga atccgccggt cgagtcggcc acctggctca
     1981 ccagttcggc gccgctggcg gtgaagccgt tctcggtgat gcgcacgaag gtggcgtcct
     2041 cccccaccga ttcgaacagc cattcgacgg tgcgcggctc gggatctccg ggccactcga
     2101 tcaggatgcg ccggcccggc tcgatggctt ttggcgtgac ggtgacggcc acgtcataca
     2161 tcgcccagcg ccactcgacg ggcttgccgg cgtcgaggcg gccggaacca tgcgtgaacc
     2221 agaagcgcgt cgtgacggcg ggatcgacga aggcctcgta gaccgtgacg accggcctgc
     2281 ggatcagcat gccggtgtgg gaggagcgga cgggttgtgg ggtcatccgc tcaggccttc
     2341 tcgatttcca cgcgcgcgtc gttgaagcag gcgttgccgc cgacgtccga gtgcgtggcc
     2401 ggcgacagcg cgttggacgt cagcccgttc ttcgtgtggc gcgcccatag acctttcggc
     2461 aggctcacga cgccggggcg tacgtccggg ttgatccggg cgatggtgaa gacctcaccc
     2521 agctcgttgt ggatgcggac cggctcgccg ttgacgatgc cgcgaggcgc cgcgtcatcc
     2581 ggatggattt ccaccggcac gtgccccggg atgagttgcg cgaacgtcga ggaaacggtg
     2641 gtgccgagcg cgggggagat cagcgccagc ggatagagag ctgtgccggg atcgggctgc
     2701 cagtgataca ggccgccggg agcctcggca tcgagatcgg gcggtacgag atgcactttc
     2761 cggtccggcg tgcccggaaa gacatgaacg aattgaatcg gattggcgcc cgtgtcgggc
     2821 gccaccagct tgtgatcggt gatcgtgtcg agcagatcgc cgtggccgtt tccatttccg
     2881 ttgccgaccg gcgtgtggcc gacaccgtcg agaatccgct tcgacagagc caccggatcg
     2941 ccggagtcct
//
LOCUS       DASVOD010000016         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_105310, whole genome shotgun sequence.
ACCESSION   DASVOD010000016 DASVOD010000000
VERSION     DASVOD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105310"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VF720_00300"
     CDS             <1..857
                     /locus_tag="VF720_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7877816.1"
                     /translation="AGTSMGIFLVAAAILEPGDRVLVEMPAYEPLVKVPLVFGAAIDR
                     LERRFEDGWQVDVDRVKSLLTPKTKIIALTNLHNPSGVRLSPEVESALVTLATERGIT
                     LFSDEVYRDFLPGPVGTAYRPGTNVVVASSLTKVYGLGGLRAGWLLGPPDLLHRASRV
                     IDMLYVNDPVPVLPYTLAAHAAADRLRERHLPLANAGQAQLDAWMRAHPDFGWVKPDG
                     GLCAFPRLPEGFTGTDVHARLLKDEGTLVVPGHFFEDDRHVRIGVGGGAEALAGGLSA
                     LERVVSRG"
     gene            1014..1298
                     /gene="groES"
                     /locus_tag="VF720_00305"
     CDS             1014..1298
                     /gene="groES"
                     /locus_tag="VF720_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX7877817.1"
                     /translation="MKFTPLGDRVLIRALEESEQKVGGIIIPDTAKEKPQQGEVVAAG
                     AGRRKKSGERVPLEVKAGDRILYGKYSGTEVNMDGQTYLILHEDEILARL"
     gene            1329..>2312
                     /gene="groEL"
                     /locus_tag="VF720_00310"
     CDS             1329..>2312
                     /gene="groEL"
                     /locus_tag="VF720_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013376916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7877818.1"
                     /translation="MAKLLQFDSEAREALRRGVDKLANVVTVTLGPRGRTVVLDKKFG
                     SPLIINDGVTIAKEIELEDPYENMGAQLVKEVSARTQDVAGDGTTTATCLAQAICTLG
                     LKSVTSGANPMALKRGIDKAVAAIVEDLKKQSKPIKTHEETAQVASISANSDRAIGEL
                     LADAMDKVGKDGVITVEEAKTLHTTFDHVEGMKFDRGYQSPYFINDADKMQVVLEDAM
                     ILIHDKKIGSIKDLVPVLEKTLQTGKPLLIISEEVEGEALATLVVNRIRGSLQVAAVK
                     APGFGDRRKAMLEDIAILTGGKLIAEEIGMSLESATLADLGKAKRIIIDKDN"
BASE COUNT          431 a          770 c          754 g          357 t
ORIGIN      
        1 tggccggcac ctccatgggg atattcctcg tggccgccgc gatcctcgag ccgggcgatc
       61 gcgtgctcgt cgagatgccg gcctacgagc cgctggtcaa ggtgccgctc gtcttcggcg
      121 cggccatcga ccgcctcgaa cgacgcttcg aggatgggtg gcaggtcgac gtcgaccggg
      181 tgaagtcgtt gctcacgccg aagacgaaga tcattgcgct caccaacctg cacaacccga
      241 gcggcgtccg cctgtcaccc gaagtggaat cggcgctcgt cacgctcgcc accgagaggg
      301 gcatcacgct cttctcggac gaggtgtatc gcgacttcct gcccgggccg gtcggcaccg
      361 cgtatcgccc cggcacgaac gtcgtggtgg cctcgagcct caccaaggtg tatggcctcg
      421 gaggtctgcg ggccggctgg ttgctcggcc cgcccgacct gctccatcgc gcgtcccgcg
      481 tcatcgacat gctctacgtg aacgatccgg ttcccgtgct cccgtacacg ctggccgccc
      541 acgccgccgc cgaccgcctt cgcgaacgcc acctgcctct ggccaacgcc ggccaggcgc
      601 aactcgacgc ctggatgcgt gcgcaccctg atttcggctg ggtgaaaccc gatggcgggc
      661 tgtgcgcctt cccgcggctg ccggaggggt ttaccggcac cgatgtccac gcgcgcctcc
      721 tgaaggacga ggggacgctc gtcgtcccgg ggcatttctt cgaggacgac cgtcacgtcc
      781 ggatcggggt cggcggggga gcggaggccc tggccggagg gctctcggcg ctcgagcggg
      841 tggtttcccg gggctgacgg cacctccccg acgacatcgc ttgaatcgcc tcgcatcgtt
      901 ctgtaatcta cgccgctggc actccatacc caagagtgcc agcggcgtct ttccattcgg
      961 cgccccatag cataagtctt ttaatggtag taacttataa acggaggttc ccaatgaagt
     1021 tcacgccttt gggcgatcgg gtcctgatcc gcgccctcga ggagtcggag cagaaggtcg
     1081 gcggcatcat cattccggac acggccaagg agaagccgca gcagggcgag gtggtggctg
     1141 ccggggccgg gcgccgcaag aagtcgggcg agcgcgtgcc gctcgaggtg aaggccgggg
     1201 atcgcatcct gtacggcaag tactcgggca ccgaagtcaa catggacggc cagacctacc
     1261 tcatccttca cgaagacgaa attctcgccc gcctctaggc gcggcgctca acgggagaca
     1321 acgagttcat ggcaaaactt ctgcaattcg acagcgaggc gcgtgaagcg ctccgccgcg
     1381 gcgtcgacaa gctggccaac gtggtcacgg tcaccttggg gccgcgcggg cgcaccgtcg
     1441 tgctggacaa gaaattcggg tcgccgctga tcatcaacga cggcgtcacc atcgccaagg
     1501 aaatcgagct ggaagacccg tacgagaaca tgggggcgca gctcgtcaag gaagtgtccg
     1561 cgcgcacgca ggacgtggcc ggcgatggca ccacgaccgc cacgtgcctg gcccaggcca
     1621 tctgcacgct gggcctgaag tccgtcacct cgggcgccaa cccgatggcg ctgaagcgcg
     1681 gcatcgacaa ggccgtggcg gccatcgtcg aggacctgaa gaagcagtcc aagccgatca
     1741 agacccacga ggaaaccgcg caggtggcct cgatctcggc caacagcgac cgggccatcg
     1801 gcgagctcct ggccgacgcg atggacaagg tcggcaagga cggcgtcatc acggtggagg
     1861 aggccaagac gctccacacc accttcgatc acgtcgaggg gatgaagttc gaccgtggct
     1921 accagtcgcc ctacttcatc aacgacgcgg acaagatgca ggtcgtcctc gaggacgcca
     1981 tgatcctgat ccacgacaag aagatcggca gcatcaagga cctggttccc gtcctcgaga
     2041 agacgctcca gaccggcaag ccgcttctga tcatctccga ggaagtggaa ggggaggcgc
     2101 tggccaccct ggtggtcaac cggatccgcg gcagcctgca ggtggccgcg gtcaaggcgc
     2161 ccggcttcgg cgatcgccgc aaggccatgc tcgaggacat cgccatcctg accggcggca
     2221 agctcatcgc cgaggaaatc gggatgagcc tcgagagcgc cacgctggcc gacctcggca
     2281 aggccaagag gatcatcatc gacaaggaca ac
//
LOCUS       DASVOD010000017         4293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_418404, whole genome shotgun sequence.
ACCESSION   DASVOD010000017 DASVOD010000000
VERSION     DASVOD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418404"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2765)
                     /locus_tag="VF720_00315"
     CDS             complement(<1..2765)
                     /locus_tag="VF720_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877819.1"
                     /translation="MKFRVLPSLAALLVLFSGAARAQDFDDPDMPPVADHGLDKATYM
                     ELRNSALMLKYSGAGFEEIYNGRIAAIEQVKVDNRHQGPFTSFSAWTPIGPYPIPNGQ
                     TTTTAHAVSGRVTAIAIDPADANTVYVGTAQGGVWRTTNGGTNWTPIFDNAASLAIGA
                     LALAPSNTSILYVGTGEANLSADSFFGVGLYRIDDAAGAATLNGPFNPTPTTDVIGAQ
                     TFTGRTISRILVDPADPATIFVSTASGIGGLGGEPYASSPPITALRGIYRSTNGTSAS
                     PSFAKLTVTPSSSIAPDVTGNLIVTDMAYDPTDATRNTIVAWVYGANSATNGGIYRTT
                     TAKGAASFTQTFITTVANTRGAFAATQAGGPTTMFVSTGESAAGTGCTTGSGVLRKST
                     DGGATWGAKLAGGGGFCGGQCFYDLPVAVSPVDANLLIIAGAGNSTCSRVLARSTNGA
                     ASFTAAGVADVGLHADGHAIEFAPSDPTIVYTGNDGGIWKSTNSGATWTSLNTAGFSA
                     TQFQSIDVHPSDANFSIGGTQDNGTNMFQPAATWNRIDFGDGGFAVIDQNAADNSNVR
                     MYHTYFNQRNNLVAYGTVSSVAAAFDGNWTLRGNNANGILVSENPNFYAPLVRGPGNP
                     NSIYYATDRLHRSVDFGVTNPVVSQAPIATSGGLGVPISAVAIAPSNDNVRLVGLNIV
                     APNPTMVWGTVTGSSTLVNMTSASFPSKYIGRIAIDPTDPNIAFVCFGGFGVPAGQHV
                     WKTTNLMTGTPTWAASGSGLPDVPVNAMVIDPVTPSKMYVGTDVGVYRSLDGGATWTA
                     FTTGMPVVSIFDMKLQPSSRILRVATHGRGMFERYVDQPPVPVDLVLVGAEIVEGHPR
                     LTWHLTDRPERLNLYRRQVPGEWSLLSQVTADNDGDIVFEDTNTKDGVSYEYQIGVIE
                     RGEETMKG"
     gene            complement(3189..3770)
                     /locus_tag="VF720_00320"
     CDS             complement(3189..3770)
                     /locus_tag="VF720_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877820.1"
                     /translation="MTPIHRLSRWSRSLVPGLLFTAALAAVSHAGGIQMSAERAEKPF
                     PRKATAAVLFVNTFECGRAHDFKLEARAEGVVNGRRSSHPLAVTPAGREGRFAIERAW
                     PLEGEWVILITTEAWNHTANLVVRLEPNGGFVPSGGAKDGSGFIEITDSETIHGELDA
                     RAVEALVAGKPAGERSEVRRPSLGEWLASSLGL"
     gene            complement(3851..>4293)
                     /locus_tag="VF720_00325"
     CDS             complement(3851..>4293)
                     /locus_tag="VF720_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877821.1"
                     /translation="LAVEPGGAGRLSVTAGWVALEDRGRQSLVPAGAACETEPGHGPG
                     TAFFLDAPETFKESLHAFDFEAGGDRALAALLAGARRRDSLTLWHLVSRVDRETTDGA
                     IDRDRIVTALAELAPMPAGVEREGILALDEGMLTRWREDLARHW"
BASE COUNT          679 a         1338 c         1560 g          716 t
ORIGIN      
        1 cccttcatcg tctcctcacc ccgctcgatc acgccgatct ggtactcgta gctcacaccg
       61 tccttcgtgt tggtgtcctc gaagacgatg tcgccgtcat tgtcggccgt gacctgggag
      121 agcaacgacc actcgccggg aacctggcgg cggtagaggt tgaggcgctc cggacggtcg
      181 gtgaggtgcc aggtgaggcg cggatgcccc tcgacgatct cggcaccgac caggaccagg
      241 tccaccggaa cgggcggctg gtccacgtag cgctcgaaca tgccgcggcc gtgcgtggcc
      301 acgcggagga tgcggctgga gggctggagc ttcatgtcga agatggacac caccggcatc
      361 ccggtcgtga aggccgtcca ggtggcgccg ccgtcgaggc tgcgatagac gccgacgtcc
      421 gtacccacgt acatcttgct cggggtgacg gggtcgatga ccatggcatt caccggcacg
      481 tccgggaggc ccgagccgga cgccgcccag gtgggcgtgc cggtcatgag gttggtggtc
      541 ttccagacgt gctggccggc cggtacgccg aagccgccga agcagacgaa ggcgatgttc
      601 gggtcggtcg ggtcgatggc gatccggccg atgtacttgg aggggaagct cgcgctggtc
      661 atgttgacca acgtgctcga accggtgacc gtgccccaga ccatggtggg gttcggcgcc
      721 acgatgttga ggccgacgag gcgcacgttg tcgttgctcg gcgcgatggc cacggcgctg
      781 atcggtacgc cgagaccgcc ggacgtggcg atcggggcct ggctgaccac ggggttcgtc
      841 acgccgaaat cgaccgaacg atggagacgg tcggtggcgt agtagatcga attcgggttg
      901 ccggggccac gcaccagcgg tgcgtagaag ttcgggttct cgctcaccag gatcccgttg
      961 gcattgttgc cgcgcagcgt ccagttgccg tcgaatgccg cggccacgct ggacaccgtg
     1021 ccgtacgcca ccaggttgtt ccgctggttg aaataggtgt ggtacatccg cacgttggag
     1081 ttgtcggccg cgttctgatc gatcaccgca aagccgccgt caccgaagtc gatgcggttc
     1141 caggtggccg ccggctggaa catgttggtg ccgttgtcct gcgtgccgcc gatggagaaa
     1201 ttggcgtcgc tcgggtggac gtcgatcgac tggaactggg tggcgctgaa cccggccgtg
     1261 ttcaggctgg tccaggtggc gccgctgttg gtcgacttcc agatgccgcc gtcattgccg
     1321 gtgtacacga tcgtcggatc cgacggggcg aactcgatgg catgaccgtc ggcgtggagg
     1381 cctacgtccg caactccggc cgccgtgaag gaggcggcgc cgttggtcga gcgggccagg
     1441 acgcggctgc aggtgctgtt gccggcgccg gcgatgatga ggagattggc gtcaaccggg
     1501 ctcaccgcaa ccggcaggtc gtagaagcac tggccgccgc agaagccgcc gccgccggcc
     1561 agcttggcgc cccacgtcgc gccaccgtcg gtggacttgc ggaggacgcc ggagccggtg
     1621 gtgcatcccg tgccggcggc cgactcgccg gtcgacacga acatggtggt cgggccgccg
     1681 gcctgcgtgg ccgcgaaggc gccgcgggtg ttggccacgg tggtgatgaa ggtctgggtg
     1741 aaggaggccg cgcccttggc ggtggtggtg cggtagatgc cgccgttggt ggccgagttg
     1801 gcgccgtaaa cccacgccac gatcgtgttg cgggtggcgt cggtcgggtc gtaggccatg
     1861 tcggtcacga tcaggttgcc cgtgacgtcc ggcgcgatgc tcgacgatgg ggtcaccgtg
     1921 agcttggcga acgacgggct ggccgaggtg ccgttcgtgg aacggtagat gccgcggagg
     1981 gccgtgatcg gcggggatga ggcgtagggc tcgccgccga ggccgccgat gccgctggcg
     2041 gtcgagacga agatcgtggc cggatcggcc ggatcgacga ggatcctgct gatcgtgcgc
     2101 cccgtgaagg tctgcgcgcc gatcacgtcg gtggtgggcg tcgggttgaa ggggccattc
     2161 aacgtggcgg cgccggccgc atcatcgatg cggtagaggc ccacgccgaa gaacgagtcg
     2221 gccgagaggt tggcctcgcc ggtgccgacg tacaggatgc tggtgttcga gggcgccagc
     2281 gccagggcgc cgatggccag cgaagcggca ttatcgaaga tcggcgtcca gttggtgccg
     2341 ccgttggtgg tgcgccacac accgccctgg gcggtgccga cgtacaccgt gttggcgtcg
     2401 gcgggatcga tcgcgatggc ggtcacgcgg ccgctgacgg catgcgccgt cgtcgtcgtc
     2461 tgcccgttgg gaatcgggta cggaccgatg ggcgtccacg cgctgaacga ggtgaacgga
     2521 ccctggtgcc gattgtcgac cttcacctgc tcgatggccg cgatgcgacc gttgtagatc
     2581 tcctcgaagc cggcgcccga atacttgagc atgagcgcgc tgttccgcag ttccatgtac
     2641 gtcgccttgt cgaggccgtg atcggccacc ggcggcatgt cgggatcgtc gaagtcctgg
     2701 gcgcgcgcgg cgcccgagaa caggaccagg agtgcggcaa gggatggcag tacccggaac
     2761 ttcatgaagt cctccttggc gtggaactgc gggtggagcg aggtgccgta gaaaaaatag
     2821 tgatcccgac tcgaggcgag gggattggat tggtggagct gaggtgctgg aacgcgacgt
     2881 catgcaggtg gatacgtccg cgccactata caggcgggtg ggggaaatcc gtagaagaat
     2941 cagaattgca tcaggattac caggaatgtg tcagggtggc cggagtccgg gccaccagct
     3001 cgcccggcga cgcgggaatt ggggctatat aaatgagggg cgtgaaccgg gtacggtgtc
     3061 acaacctgaa tacttccaat taattagcgt cgaaaccggg aattttttcc catggcggca
     3121 aagacggttt cccggggtcg aagcgccgcc aggctcttct cccccgggat tcaaagggac
     3181 tcggccggct aaagcccgag ggatgacgcc agccactcgc cgagcgacgg tcgccgcact
     3241 tccgaccgtt cgccggccgg ctttcccgcc accagcgcct cgaccgcccg ggcatccagc
     3301 tccccgtgga tcgtctccga gtcggtgatc tcgatgaaac cggagccatc cttcgcaccg
     3361 ccgctcggca cgaatccccc gttcggttcg aggcgcacca cgaggttggc cgtgtgattc
     3421 caggcctcgg tcgtgatgag gatcacccat tccccctcca gcggccaggc gcgctcgatg
     3481 gcgaagcgac cctccctgcc ggccggtgtg acggcgagcg ggtggctcga gcgcctgccg
     3541 ttcaccaccc cctcggcgcg cgcctcgagc ttgaagtcgt gggccctgcc gcactcgaac
     3601 gtgttgacga aaaggaccgc tgccgttgcc ttgcggggaa acggcttctc ggctcgttcc
     3661 gcggacatct ggatgccgcc ggcatgggag accgcagcga gggccgcggt gaacagcaat
     3721 cccgggacaa gcgaccggga ccagcgggaa aggcgatgga tcggtgtcat ggtgtcctcc
     3781 tcattcaggg ttgggcgccg ggcatggctc cgcccggagc gttgcgggtg agagtcgaaa
     3841 acggtgcccc ctaccagtgg cgggcgaggt cctcgcgcca tcgggtgagc atcccctcgt
     3901 cgagtgcgag gatcccctcg cgctcgacgc ctgcgggcat cggcgccagt tccgccaggg
     3961 cggtgacgat ccgatcgcgg tcgatcgcac catcggttgt ctcccggtcg acgcgggaaa
     4021 cgagatgcca gagcgtgagg ctgtcgcgcc ggcgcgcgcc ggccaggagg gcggccagcg
     4081 cgcggtcacc gccggcctcg aagtcgaacg cgtgcagcga ttccttgaac gtttccggcg
     4141 catcgaggaa gaaggccgtt cccggcccat gccccggctc ggtctcgcaa gccgcaccgg
     4201 ccggcaccag cgactggcga ccccggtctt ccagcgccac ccaaccggcg gtcaccgaca
     4261 gtcgaccggc gccaccgggc tccacggcca gcg
//
LOCUS       DASVOD010000018         5014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_470606, whole genome shotgun sequence.
ACCESSION   DASVOD010000018 DASVOD010000000
VERSION     DASVOD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470606"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF720_00330"
     CDS             complement(<1..291)
                     /locus_tag="VF720_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877822.1"
                     /translation="MRPGRCRLSPYRLFRQWLLRAVAAACLFLAGPVSASEPWMWDQD
                     ENGLDDRLTSAFINGISAVYENGDPDGRLRFEVTFVDTLLQYGGYVRFNHTPT"
     gene            complement(302..3709)
                     /locus_tag="VF720_00335"
     CDS             complement(302..3709)
                     /locus_tag="VF720_00335"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX7877823.1"
                     /translation="MARTYLQVVEARLARPRLPPILLRDRLIDQLRRGVPAHPLTLVT
                     AGAGYGKTTLMASATDNAPESTLWYSLGEEDADLTRFLRHVGAALQRHNRRYGRALKA
                     MLDEGTIEARSAATAAGALLNDLGRIKESMVLVLDDFHLVQGSKEIQEFLSIVVENNR
                     SPVHLMIASRTEPPLPLGRLRARRMVLELGPADLAFTAAELKSLLEEVHHRPMSEDDI
                     DFLARFTEGWVTPIQLALQADGLNAPRDLPRTLRRAMASGATLHDYLAAEVLDRQPPG
                     WRRWILETSPFEEIEPELLRTVLGEPDPEGRLGELLRMSLIQAFEGETGTVYRYHVLL
                     RELLVRRFQVEVPEADRDRLHRAGAAWFRRRGDLVGAARQLAFLDDRSVLAEFLRAEG
                     LALLDAGHYQALTHWLDQLPAHLVDEDAWLRLRRADARHFLADWPGAELDYEKARALF
                     AAASDSRGEAWAILGLARLWNLRGQSLLAVEEGEAALRQLASTGDNSAEELEVRLNKV
                     VSGAYYYQGRYADAIRGLDRLEELARGHPERQATLWNNRAVVHASQGNYPEAAAAFEK
                     GLRRPGARQSPRAPLHLSNLGLLLNEMGDPERARVLFDEALRRARHWKNRSHQLSALL
                     GQAHLLHRLGNTEGCLTLLKEVDELNDELKIPLLVSDAMALRARVLAESNQHAAAREL
                     LRGALAAWGTTTRDSHWLLYRVEDAVIDLKSGRTEEALAAFDELRPVAFELEALFPRM
                     RLLFHRGEAAMLEGLPDAESELTAALELGQRLGYDAFLRAEFRRDPKPFLFLLKRGVA
                     SDYLARLAIGAGAALELEFLDLVGDATLPPVAGKTVLRILAETGGPPAHRRISASSWT
                     ADGPLTRAARAALDSIERRHPDLTTLRPTGRRALIISLLGPLKMTAPTGELEAARWTS
                     QRALSLFLYLVLRGDRGVSRDRLIELFWPGRQRRGAESNFHPTLSYVRRALKDVAAGQ
                     VVVTVNGLYQVDPDLPITIDVRHFEALASEGRARGKSRDRIQALEEAARLYRGDLLED
                     REEPWADDLRTQLGLRYEGVLAELAGLLLSSGDAGRAIPHARTLLARSPYREEVHLLL
                     MRCYNLAGDRQAVHEQYRRMVRLLREDLGIEPLPETTRSYESLVAAS"
     gene            complement(3750..4409)
                     /locus_tag="VF720_00340"
     CDS             complement(3750..4409)
                     /locus_tag="VF720_00340"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7877824.1"
                     /translation="MADRNPTPGGAAPWFFLGAVYLGLEAYLWLVVPTRNSLLIAIGA
                     IVVLFVLVGDPIRRGETADSVGLDVTHLRQATLRLLPMTALAMIVLFAVNQLVAAPPP
                     DLPRFGRRFLAILPWAFLQQGMLQATFNRRITARLGPGWRAAGLVALAFAGMHLPGPL
                     LVAITGLAGLVWARTYQSAPNLWVLVLSHALLSAMAQSSLPREWTHGFRVGPGWFRWH
                     P"
     gene            4478..>5014
                     /locus_tag="VF720_00345"
     CDS             4478..>5014
                     /locus_tag="VF720_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877825.1"
                     /translation="MTTDSSPRPSDGTNPSQSSPDSKPRVAYFCMEYGLSEKFPIYSG
                     GLGVLAGDYMKSAGDLGLPVVGIGLLWNNATHQTLDDNGQAVDEWREYPHDYLEDTHV
                     RVRVRIRAREVECRVWRVTGFAIAPLYLIEPVHKEDKWITERLYDTRPDCRVAQEMML
                     GIGGVRALSRLGLTIDVYH"
BASE COUNT          843 a         1612 c         1810 g          749 t
ORIGIN      
        1 cgtgggtgtg tggttgaacc tgacgtagcc gccgtactgc aggagggtgt cgacgaaggt
       61 aacttcgaag cggagccgcc catccggatc gccgttttcg tagacggccg agatgccgtt
      121 gatgaaggcg ctggtcaggc ggtcatccag gccgttctcg tcctggtccc acatccaggg
      181 ctcggaggcg gaaacgggcc cggccaggaa caggcatgcg gcggccacgg cccggaggag
      241 ccactggcga aacaacctgt acggggagag ccggcagcgg cctggtctca tacggcgatc
      301 ctcaggaagc ggcgaccagg gattcgtacg accgcgtcgt ctcgggcagt ggctcgatgc
      361 ccaggtcctc gcggaggaga cggaccatcc ggcgatactg ttcgtgcacg gcctgacgat
      421 cgcccgcaag attgtaacat cgcatcagca gcagatggac ttcttcgcga tacggactgc
      481 gcgccagaag cgtcctggca tggggaattg ccctacccgc atccccggac gaaaggagga
      541 ggccggccag ttcggccagc accccttcgt accggagccc gagctgagta cggaggtcgt
      601 cggcccacgg ttcttcccgg tcctcgagga gatcgcctcg gtagagccgg gcagcctcct
      661 cgagggcctg gattcgatca cggctcttcc cccgggcgcg cccctcggag gcgagggctt
      721 cgaaatggcg cacatcgatc gtgatgggga ggtcggggtc gacctggtag aggccgttca
      781 ccgtcacgac gacctggccc gccgccacgt ccttcaaggc ccgccggacg tacgacagcg
      841 tcgggtgaaa attactctcc gccccccgac gttgccgacc gggccagaag agctcgatga
      901 gccggtctcg cgacacgccg cgatcgccac ggagcacgag gtagagaaac agggaaaggg
      961 cgcgttggga ggtccagcgg gccgcctcga gttcaccggt gggagccgtc atcttgagcg
     1021 ggccgagcag ggagatgatg agcgcgcgac gtccggtcgg gcggagtgtg gtcaggtcgg
     1081 gatgtcgtcg ctcgatgctg tcgagcgcgg cccgggcggc gcgggtgagg gggccgtctg
     1141 ccgtccacga ggatgccgag atgcgccgat gcgccggcgg tccacccgtc tcggccagga
     1201 tgcggagcac cgtcttgccg gccaccggtg ggagggtggc atcgccgacc aggtcaagga
     1261 attcgagttc gagcgcggcg ccggcgccga tcgccaggcg ggccaggtag tcggaggcca
     1321 cacccctctt gagcaagaag aggaagggct tcggatcgcg acggaattcg gcgcggagga
     1381 aggcgtcgta gccgagccgc tggcccagtt cgagcgcggc ggtgagctcc gactcggcat
     1441 cgggaagccc ctcgagcatg gccgcctcgc cgcggtggaa gaggaggcgc atgcggggga
     1501 agagcgcctc cagttcgaac gccaccgggc gcaattcgtc gaacgcggcc agtgcctcct
     1561 ctgttcgccc cgacttcaag tcgatgaccg cgtcttccac gcggtagaga agccagtgcg
     1621 aatcgcgcgt ggtggttccc caggcggcca gcgccccgcg caggagctcg cgcgccgcgg
     1681 cgtgctggtt cgattcggcg agcacgcggg cccgcagcgc catcgcatcc gagaccagca
     1741 ggggaatctt cagttcgtcg ttgagttcat ccacttcttt gagcagcgtg aggcacccct
     1801 cggtgtttcc cagtcgatgg agcaggtgcg cctggccgag cagcgccgac agctggtggc
     1861 tccggttctt ccagtgacgc gcgcggcgca acgcttcgtc gaacagcacg cgcgcgcgtt
     1921 cgggatcgcc catttcgttg agcaggagac cgagattcga gaggtggagc ggggcgcggg
     1981 gagattgacg ggcccccggt cggcgcagcc ccttctcgaa ggcggcggcc gcctcgggat
     2041 agtttccctg gctggcgtgc acgacggcgc gattgttcca gagcgtggcc tgccgttcgg
     2101 gatggccgcg cgccagctct tcgagccgat cgaggccgcg aatggcgtcg gcgtagcgcc
     2161 cctggtagta gtaggcgccc gacaccacct tgttgagccg cacctcgagt tcctcggcgg
     2221 agttgtcgcc ggtcgaggcc agttgccgga gtgccgcttc accttcctcg accgcgagga
     2281 gagactggcc gcgcaggttc cagagtcggg ccaggcccag tatggcccac gcctcgccac
     2341 gcgagtccga tgccgcggca aagagcgccc gtgccttttc gtagtcgagc tcggcgccgg
     2401 gccagtcggc caggaagtgg cgggcatcgg cgcggcggag gcggagccag gcgtcctcat
     2461 ccaccaggtg agcgggcaat tgatcgagcc agtgggtcag ggcctggtaa tgaccggcgt
     2521 cgagaagcgc cagtccttcc gcgcggagaa attcggccag cacgctccgg tcgtcgagga
     2581 aggccagctg gcgcgcggcg cccaccaggt cgccgcgacg gcgaaaccag gcggcgccgg
     2641 cgcgatgcag gcggtcgcgg tcggcttcgg gcacctccac ctggaaacgc cggacgagga
     2701 gttcgcgcag cagcacgtgg taccgataga ccgtgcccgt ttccccttcg aacgcctgga
     2761 tcaggctcat ccggaggagc tcgccgagcc gcccttccgg gtcgggctcg ccgagcacgg
     2821 tccggagcag ctccggttcg atctcctcga acggtgaggt ctcgaggatc catcggcgcc
     2881 accccggagg ttgacgatcg aggacttcgg cggccaggta gtcgtggagc gtggccccgg
     2941 aggccatcgc ccggcgcaac gtgcgtggca ggtcgcgcgg ggcattcaac ccgtcggcct
     3001 ggagcgccag ctggatcggc gtcacccacc cctcggtgaa gcgcgccagg aaatcgatat
     3061 cgtcctccga catcggacgg tggtgcacct cctcgagcag cgacttgagc tcggcggcgg
     3121 tgaatgccag gtcggccggg cccagctcga gcaccatgcg tcgcgcgcgg aggcggccga
     3181 gcggcagggg cggctcggtc cggctggcga tcatcaggtg cacggggctc cggttgttct
     3241 cgacgacgat cgagaggaac tcctggatct ccttcgatcc ctgcaccagg tggaagtcgt
     3301 ccagcacgag caccatcgat tccttgatgc ggccgagatc gttgaggaga gcgccggcgg
     3361 ccgtggcggc cgagcgggcc tcgatcgtcc cctcgtcgag catcgccttg agggcccggc
     3421 cgtaacggcg gttgtggcgc tggagcgcgg cgccgacgtg gcgcaggaac cgggtgaggt
     3481 cggcgtcttc ttcgccgagc gaataccaga gggtcgactc gggcgcgttg tcggtggccg
     3541 aggccatgag ggtggtcttg ccgtatcccg cgccggcggt gacgagcgtg agcgggtgcg
     3601 ccggcacccc ccggcgcaac tgatcgatca ggcgatcacg tagcaggatg gggggcaggc
     3661 gggggcgggc gagtcgggcc tcgacaactt gaaggtaggt tctggccatt gggtctccaa
     3721 ccgggacccg tcaattgtac ctgccggcct cagggatgcc agcggaacca gccgggcccc
     3781 acccggaaac catgcgtcca ctcccgcggg agggagctct gcgccatggc cgacaggagc
     3841 gcgtgggaaa gcacgagaac ccagagattg ggcgccgact ggtacgtgcg ggcccagacg
     3901 agaccggcca ggcccgtgat cgccaccagc agggggccgg ggagatgcat tcccgcgaag
     3961 gccagagcca cgaggccggc cgcgcgccac ccggggccca gccgggccgt gatccgccgg
     4021 ttgaaggtgg cctgcagcat tccctgctgg aggaacgccc aggggaggat ggccaggaag
     4081 cggcggccga agcggggcaa gtcgggaggc ggcgccgcga ccagttggtt cacggcgaac
     4141 aacacgatca tggccagtgc ggtcattgga aggaggcgaa gcgtagcctg gcgcagatgc
     4201 gtgacatcga gcccgaccga gtcggccgtt tcgccgcggc ggatcgggtc gccgaccagc
     4261 acgaagagaa ccacaatcgc gccgattgcg atgagaagcg aattccgggt ggggacgacc
     4321 aaccagagat aggcctccag gccgaggtac accgcgccga gaaagaacca gggggcggcg
     4381 ccgccggggg ttgggttgcg atcggccatg atcgagacaa tagtgcggtg cgttccaact
     4441 tgaaaccgtg ttgtcactcg gggataatca ttcgctcatg acgacagact cctcgccacg
     4501 cccctccgac gggaccaacc cgtcccagtc ctcccccgat tcgaagcccc gtgtggccta
     4561 tttctgcatg gaatacggcc tctccgagaa attcccgatc tactcgggcg gcctcggcgt
     4621 gctggccggc gattacatga agtcggccgg ggacctcggc ctgcctgtcg tcgggatcgg
     4681 cctcctctgg aacaacgcca cgcaccagac actcgacgac aacggccagg cggtggacga
     4741 atggcgggag tacccgcacg actacctcga ggacacccat gtcagggtcc gggtgcgcat
     4801 ccgcgcccgc gaggtggaat gccgggtctg gcgtgtaacg ggtttcgcga tcgcgcccct
     4861 ctatctcatc gagccggtcc acaaggaaga caagtggatc accgagcgcc tctacgacac
     4921 gcgcccggat tgccgcgtgg cgcaggaaat gatgctcggc atcggcggcg tgcgggcgct
     4981 ctcgcgcctc gggttgacga tcgacgtgta ccac
//
LOCUS       DASVOD010000019         2064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_940270, whole genome shotgun sequence.
ACCESSION   DASVOD010000019 DASVOD010000000
VERSION     DASVOD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940270"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF720_00350"
     CDS             <1..355
                     /locus_tag="VF720_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877826.1"
                     /translation="AWVEAYDRRVWRGEAPADGMLASAEQERDAAYGELTRDNYLDVT
                     FPPAGLAVTNGRASRGTRVPIPDLMPGEYDLVMTITDLHAKSSATASTWFQVLSPEQR
                     ADLVSMARGDSGHSR"
     gene            581..1897
                     /locus_tag="VF720_00355"
     CDS             581..1897
                     /locus_tag="VF720_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877827.1"
                     /translation="MFDNSLFSYGRRGIALALLVTGSFLVTGCGDDDDDVDDDVSAVS
                     GRVDGGVGGTAPAQIEGATVSLARINSSGNLETVSSEAATSDAEGRFRVETDLDNVRY
                     LVVVADKGSTHLEAVVADAVQNGSEIKSQPLTQETTVEARVWRAIVADNKTSSVSLAD
                     VATYITPSVASGIAGNAAAIEAIATALEAEGDVRNAALADAEFGVTTTELNVINQARA
                     NALANLDEALLAADTQAEIDAAWQAWYTADAAAYATGSVDLDAILRAREIASRALLNS
                     TGSLSPSVELSVVRGAALARAAVLTMATQDAFLDANATAGERTTVANAGTTLINAIKA
                     ATTEQAILNAFDTYHDTLLARLQVVYPLRSTALTTVNDALYASSGPRATLLTTVGTST
                     NPTVLVPAYVDYFNSATTAVSTSLVGAPQAEIDIATRVLLDTSLIM"
     gene            complement(1903..1979)
                     /locus_tag="VF720_00360"
     tRNA            complement(1903..1979)
                     /locus_tag="VF720_00360"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Arg,seq:tct)
BASE COUNT          356 a          700 c          684 g          324 t
ORIGIN      
        1 cgcctgggtg gaggcctatg accggcgggt gtggcggggc gaagcaccgg ccgatggcat
       61 gctggcctcg gcggagcagg agcgggacgc ggcgtacggc gaactgaccc gcgacaacta
      121 tctcgacgtg acctttccgc cggccggcct tgccgtgacg aacgggcgcg cctcccgcgg
      181 cacgcgggtg ccgattcccg acctcatgcc gggagagtac gacctcgtga tgacgatcac
      241 cgatctccac gcgaagtcgt cggccacggc cagcacctgg ttccaggtgc tctcgccgga
      301 acagcgcgcg gacctggtct cgatggcgcg cggggactcg gggcactccc gataggaaag
      361 atcgcccggg aactttgatc catcccgcca ttgaattgag cccggccgcg taagccttgc
      421 ccgtcatgcc ggcgcatttc ccgcgctccg gtcacgtgtg ctggcatgac ccgtgcgtgt
      481 tacgcgcgcg ggcgttccgg ttcgttcccg catacgaacg tcttccttcg ggatatcccc
      541 gattgttcag cgctgattcc ggccgcggag gaggaatttc atgttcgaca attcgctgtt
      601 ttcgtacggg cgtcggggta tcgccctggc cctcctggtg accggttcgt tcctcgtgac
      661 cggctgcggt gatgatgatg atgacgtgga tgacgacgtt tccgcggtct ccggccgcgt
      721 ggacggcggc gtcggcggca ccgccccggc acagatcgaa ggggccacgg taagcctggc
      781 ccgcatcaac tcatcgggca acctcgagac ggtgtcgagc gaggccgcca ccagcgatgc
      841 cgaagggcgt ttccgcgtgg aaaccgacct ggacaacgtg cgctacctgg tggtcgtggc
      901 cgacaagggc tccacccacc tcgaggccgt ggtggccgac gcggtgcaga acggcagcga
      961 gatcaagagc cagccgctga cccaggaaac caccgtcgag gcgcgcgtct ggcgcgcgat
     1021 cgtggcggac aacaagacgt cgagcgtttc gctggccgac gtggccacgt acatcacccc
     1081 gagcgtggcc tctggaatcg ccgggaatgc cgccgccatc gaagccatcg ccaccgccct
     1141 cgaggccgag ggtgacgtgc gcaatgccgc gctggccgac gccgagttcg gcgtgaccac
     1201 cacggagctc aacgtcatca accaggcccg ggccaatgcc ctggccaacc tcgacgaggc
     1261 gctcctggcg gcggataccc aggccgagat cgacgccgcc tggcaggcct ggtacaccgc
     1321 cgacgccgcg gcctatgcca ccggttcggt ggacctcgat gccatcctcc gggcgcgcga
     1381 gatcgcctcg cgggcactac tcaattccac aggtagcctg agccccagcg tcgagctctc
     1441 ggtcgtgcgg ggcgccgcgc tggcccgtgc cgcggtgctg acgatggcca cacaggacgc
     1501 cttcctcgac gccaacgcca ccgccggtga gcggaccacc gtggccaatg ccggcacgac
     1561 gctgatcaac gccatcaagg cggccacgac agaacaggcc attctgaacg cgttcgacac
     1621 gtatcacgac acgctgctgg cccggctcca ggtggtgtat ccgctccggt cgacggccct
     1681 cacgaccgtc aacgacgcgc tgtacgcgtc gtccggtccc cgcgccacgc tcctgaccac
     1741 cgtgggcacg tccaccaatc cgaccgtgct ggtgccggct tacgtcgact acttcaacag
     1801 cgccacgact gccgtctcga cgtcgctggt cggcgcgccg caggcagaaa tcgacatcgc
     1861 gacgagggtg ctgctggata cgtcgttgat catgtaagga agtggcgcgc ctggagggaa
     1921 tcgaaccccc gaccgacgga ttagaaatcc gttgctctat ccgactgagc tacaggcgcg
     1981 tgagtgcaga agagtagcga accagaagaa tgagcgcacg atgacggggg gagaagggcg
     2041 gctcgggtgc tcgataacgg taag
//
LOCUS       DASVOD010000020         4729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_783664, whole genome shotgun sequence.
ACCESSION   DASVOD010000020 DASVOD010000000
VERSION     DASVOD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783664"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2336)
                     /gene="lon"
                     /locus_tag="VF720_00365"
     CDS             complement(<1..2336)
                     /gene="lon"
                     /locus_tag="VF720_00365"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7877828.1"
                     /translation="MLGREPVVPDVVPIFLLREVVAFPFMMFPLVATEPAQVELADEA
                     LAGSKTIGLFTELDEDTVRPAGSSPLDNLPFHRVGTAAVIHKMPRLPDGGMRLLVQGT
                     SRIRVTKVVQSDPFWKARVEPVPTIEVAGLRTEAQTRNLKELFARIIDGSNTMPDELK
                     VIAFNIDEVTRLVDFISANLSILTPAKQEILETPSLEKRIELITGHLHHELNLQDMHK
                     KIQSEVQSEIDKSQKDYILRQQIKAIQKELGEGEDAPTELVELEARIKTTVMPDAALE
                     AATKEMDRLKRMSPGAAEYSVARTYIEWILTVPWALPVRKPIDLNKAERILNEDHYDL
                     ERVKQRILEYLAVRKLKAEARSPILCLVGPPGVGKTSLGKSIAKALDRPFVRLSLGGV
                     QDEAEIRGHRRTYVGAMPGRILSGLVTAKAPDPVFILDEIDKVGLSYRGDPTSALLEV
                     LDPEQNGTFSDNYLNIPVDLSQVIFITTANMLDTIPPPLRDRMEVIDIDGYASQDKVF
                     IARNYLIPRALDDNGLKKGQLSFTEKALIRIIQGYTMEAGVRNLERELSRVARKVATR
                     VARRKTAPVTVTEAKVPDYLGPEPFFEERSGRKDAVGVATGMAWTPVGGTILFVEATR
                     MPGSRNLTLTGQLGDVMRESAIAALSYLRTHATGYGIEEDEFNNYDVHIHVPAGAMPK
                     DGPSAGVAMFTALTSLFTGLPVRRDTAMTGELTLRGQVLPIGGVRQKVLGAHRSGIKR
                     IILPRANSRDLEDVSPEVRKALTFIFVDDLDDALRAAFP"
     gene            complement(2380..2802)
                     /locus_tag="VF720_00370"
     CDS             complement(2380..2802)
                     /locus_tag="VF720_00370"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7877829.1"
                     /translation="MSSWEREWQHLREMMEHMLQDSTRTTATAGIVWHPPTDAWETDR
                     EFVIRVDVAGVRRDDLKVLIQGGEMQIRGIRRDAVPPGKKHFYKMEITLGPFERRVPL
                     PRGARFSEVQATYRDGILEIRCQKAHGEVPGEVRIDIA"
     gene            complement(2815..3408)
                     /locus_tag="VF720_00375"
     CDS             complement(2815..3408)
                     /locus_tag="VF720_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HEX7877830.1"
                     /translation="MTERENDPPPPGGEAPRSGEGHPPESAWGQPPSNETPIDVPIHD
                     EPIDDVPIDDEPALSDQASPGGPADAEHLAHEVGLIFENAVEAIRADDGRYRMEAYQF
                     VREALDWQVSRMSIRRHLSGPELLEGVKKLAWDRFGPMARTVLNHWGLVDGEDVGRIV
                     FQLVDAGILSKTEDDRLDDFAGVVRFDDAFEAGYRWP"
     gene            complement(3405..>4729)
                     /locus_tag="VF720_00380"
     CDS             complement(3405..>4729)
                     /locus_tag="VF720_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7877831.1"
                     /translation="HSRRRILHAADQTGREIERALLAAVAARPNIRLLENQLALDLII
                     ESRLLGTGRPGAGAETAWGAYILDEATGLVEPYMAAVTVVATGGTGKVYRYTSNPDIA
                     TGDGLAMGFRAGATVGNLEFVQFHPTCLYHPEARSFLISEAVRGEGAVLRTLDGNAFM
                     TGYDPRGDLAPRDIVARAIDSELKRRGESHVLLDAVHLGEKVLLEHFPNIVSTCRSFG
                     IDPVRRPIPVVPAAHYMCGGLVTDRDARTDIHRLLAVGEVAMTGLHGANRLASNSLLE
                     ALVFAGRAALTARSMSPGTRVPRPVPWVAPPATERKEKVIVDHNWDAVRGLMWDYVGI
                     VRSEERLKSALARLHLIRDEVESFYRRFPVDADLAELRNITLVAEMIIRCATYRKESR
                     GLHYTVDHPNRDDGAFARDTLLSSTQGLRLGPPVDHASEAGVPEGAPS"
BASE COUNT          721 a         1542 c         1620 g          846 t
ORIGIN      
        1 gggaaggccg cccggagcgc gtcgtccagg tcgtccacga agatgaaggt gagcgccttc
       61 cgcacctcgg gcgacacgtc ttccaggtcg cgcgagttgg cgcgcggcag gatgatccgc
      121 ttgatcccgg accggtgcgc accgagcacc ttctgccgga ccccgccaat cggcagcacc
      181 tgcccccgca gcgtgagctc accggtcatg gccgtgtcgc gtcggaccgg caggccggtg
      241 aagagcgagg tgagcgcggt gaacatggcc acgccggcgg agggaccatc cttgggcatg
      301 gcgcctgccg gcacgtggat gtgtacgtcg tagttattga actcgtcttc ctcgatgccg
      361 tagccggtgg cgtgggtccg gaggtacgag agcgcggcga tggccgattc tcgcatcacg
      421 tctcccagtt gcccggtgag cgtgaggttc cggcttccgg gcatccgcgt ggcttccacg
      481 aagaggatgg tgccgccgac cggcgtccac gccatgccgg tggccacccc caccgcgtcc
      541 ttgcggccgc tccgctcctc gaagaagggc tcgggcccca ggtaatccgg caccttggcc
      601 tcggtgacgg tgaccggcgc ggtctttcgg cgcgccacgc gggtggccac cttgcgggcc
      661 acgcgcgaaa gctcccgttc caggttccgg accccggctt ccatcgtgta cccctggatg
      721 atccggatca gcgccttctc ggtgaacgag agctgcccct tcttgaggcc gttgtcatcc
      781 agtgcgcgcg ggatcaggta attccgggcg atgaacacct tgtcctgcga ggcgtagccg
      841 tcgatgtcga tgacctccat ccggtcgcgc agcggcggcg ggatggtgtc gagcatattg
      901 gccgtggtga tgaagatgac ctgcgacagg tcgaccggaa tgttcaggta gttgtcgctg
      961 aacgtgccgt tctgctccgg gtcgagcact tcgaggaggg ccgacgtcgg atccccccgg
     1021 tacgagaggc ccaccttgtc gatttcatcg aggatgaaga ccgggtcggg cgccttggcc
     1081 gtgacgaggc cggaaaggat gcgccccggc atggcgccca cgtaggtgcg ccgatggccg
     1141 cggatttcgg cctcgtcctg gacgccgccg agcgagaggc gcacgaaggg acgatcgagc
     1201 gccttggcga tcgacttgcc gagcgaggtc ttgccgacgc cgggcgggcc gaccaggcag
     1261 aggatgggcg accgggcctc cgccttgagc ttgcgcaccg ccaggtattc gaggatgcgc
     1321 tgcttgaccc gttccaggtc gtagtgatcc tcgttgagga ttcgctcggc cttgttcagg
     1381 tcgatcggtt tccggaccgg gagcgcccat ggcacggtga ggatccactc gatgtaggtg
     1441 cgggccacgc tgtactcggc agcccccggc gacatcctct tcagccggtc catttccttg
     1501 gtagccgcct cgagcgccgc gtcgggcatc acggtcgtct tgatgcgtgc ctcgagctcg
     1561 acgagctcgg tgggcgcgtc ctccccttcc cccagctcct tttgtatggc cttgatctgc
     1621 tggcggagga tgtaatcctt ctgggacttg tcgatctcgc tctgcacctc ggactgtatc
     1681 ttcttgtgca tgtcctggag gttgagttcg tggtgcaggt gcccggtgat cagctcgatc
     1741 cgcttctcca gggagggtgt ctcgaggatt tcctgcttgg ccggcgtcag gatggagagg
     1801 ttcgccgaga tgaagtcgac cagccgcgtc acttcgtcga tgttgaaggc gatcaccttg
     1861 agctcgtccg gcatcgtgtt tgaaccgtcg atgatgcggg cgaagagctc tttcaggttg
     1921 cgggtctgcg cctcggtgcg caggccggcc acctcgatcg tgggcaccgg ctccacgcgg
     1981 gccttccaga acggatcgga ctggacgacc ttcgtgacgc ggatgcggct ggtcccctgc
     2041 acgaggaggc gcatgccgcc gtcgggaagg cggggcatct tgtggatgac ggcggcggtt
     2101 cccacccggt gaaacggcag gttgtcgagc ggactcgatc cggccgggcg gacggtatcc
     2161 tcgtcgagtt cggtgaagag accgatcgtc ttgctcccgg ccagcgcctc gtcggccagc
     2221 tccacctggg cgggctcggt ggccaccagc ggaaacatca tgaacgggaa cgccacgact
     2281 tcccggagga ggaagatcgg cacgacatcg ggcacgaccg gttcgcgccc gagcacggga
     2341 tccgtgccgg cggaatcgga cgggcgttcg accgggatgt caggcgatat cgatgcggac
     2401 ttcgcccggg acttcgccgt gagctttctg gcagcggatt tcgaggatgc catcgcgata
     2461 tgtggcctgg acttccgaga agcgggcgcc ccgtggaagc ggcacgcgtc gctcgaacgg
     2521 tccgagcgtg atctccatct tgtagaagtg tttcttgccg ggcggcacgg cgtcgcgccg
     2581 tatgccgcgg atctgcattt caccgccctg gatcagcact ttcaggtcgt cacgccggac
     2641 accggccaca tccacccgga tcacgaactc gcgatcggtt tcccacgcgt ccgtcggtgg
     2701 atgccagacg atgcccgctg tagcggtcgt tcgcgtggag tcctgcaaca tgtgttccat
     2761 catctcgcgc aggtgctgcc actcccgctc ccatgagctc atgacatcag ttcctcatgg
     2821 ccagcggtac ccggcctcga acgcgtcgtc gaatcggacg acgccggcaa aatcatccag
     2881 tcgatcatcc tcggtcttgc tgaggatacc cgcatcgacc agctggaaca cgatccgccc
     2941 cacgtcctcg ccgtcaacca atccccagtg attgaggacg gtgcgcgcca tcggcccgaa
     3001 acgatcccag gcgagcttct tgaccccctc caggagttcc ggccccgaga gatggcggcg
     3061 tatcgacatc cgggacacct gccagtcgag cgcctcgcgg acgaactgat aggcctccat
     3121 ccggtagcgg ccgtcatcgg cccggatggc ctcgacggca ttctcgaaga tcaatccgac
     3181 ctcgtgggcc aggtgctccg cgtcggccgg accacccggc gatgcctgat cggaaagggc
     3241 cggctcgtcg tcgatcggca cgtcgtcaat cggctcgtcg tggatcggca cgtcgatcgg
     3301 cgtctcgttg gatgggggct ggccccatgc ggattcgggc gggtgcccct ctccggagcg
     3361 cggcgcctcg ccgcccgggg gcgggggatc gttttcgcgt tccgtcatga cggggctcct
     3421 tcggggacac cggcctcgct ggcatggtct accggcgggc cgagccggag accttgagtg
     3481 gacgacaaca gcgtatcacg cgcgaaggct ccgtcgtccc ggttcgggtg atccaccgtg
     3541 tagtggagcc cccggctctc tttccgatag gtggcgcagc ggatgatcat ttcggccacc
     3601 agcgtgatat ttctcagctc ggccaggtcg gcgtcgaccg gaaaacgacg gtagaacgac
     3661 tccacctcgt cacggatcag gtgcagtcgg gccagcgccg acttgagtcg ctcctcgctg
     3721 cggacgatcc ccacgtagtc ccacatgagg ccgcgcacgg catcccagtt gtggtcgacg
     3781 atgacctttt ccttccgctc ggtggccggc ggcgccaccc atggcaccgg gcgcggcacg
     3841 cgggtaccgg gggacatgct ccgcgccgtc agggcggcgc gcccggcaaa gaccagcgcc
     3901 tcgaggaggc tgttggaggc caggcgattg gcaccatgga ggccggtcat cgccacctcc
     3961 ccgaccgcga ggaggcgatg gatgtcggtg cgcgcatcgc gatcggtgac gagaccgccg
     4021 cacatgtagt gcgctgccgg cacgacgggg atcggccgcc ggaccgggtc gatgccgaac
     4081 gaccggcagg tggagacgat gttcgggaag tgctcgagca gcaccttctc tcccaggtgc
     4141 acggcgtcga gcagcacgtg cgattcgcca cgccgcttca gttcgctgtc gatggcccgg
     4201 gccacgatgt cgcgcggggc gagatcaccg cgcgggtcgt agccggtcat gaaggcgttc
     4261 ccgtcgagtg tcctgagcac cgcaccttca ccgcgcaccg cctcggagat gaggaacgag
     4321 cgggcctccg ggtgataaag gcaggtggga tggaactgga cgaattccag gttccccacc
     4381 gtggcccccg cgcggaagcc catggccagc ccatcccccg tggcgatgtc ggggttcgag
     4441 gtgtagcgat agaccttccc cgtgccgccg gtggccacca ccgtgaccgc cgccatgtac
     4501 ggctccacga ggcccgtggc ctcgtcgagg atgtaggcgc cccacgccgt ctcggcaccc
     4561 gcgccggggc gcccggtgcc gaggaggcgc gattcgatga tcaggtcgag ggccagctgg
     4621 ttttccagga ggcggatgtt gggacgggcc gcaacggccg ccaacagcgc cctttcgatt
     4681 tcccggccgg tctggtcggc ggcatggagg atgcggcgcc gggagtggg
//
LOCUS       DASVOD010000021        11121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_783670, whole genome shotgun sequence.
ACCESSION   DASVOD010000021 DASVOD010000000
VERSION     DASVOD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11121
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783670"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1573
                     /gene="kdpB"
                     /locus_tag="VF720_00385"
     CDS             <1..1573
                     /gene="kdpB"
                     /locus_tag="VF720_00385"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020507045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX7877832.1"
                     /translation="VRESGGDFSAVTGGTRVLSDWLVIRVTAGAGQSFLDRMIALVEG
                     SKRRKTPNEIALDILLAGLTIIFLVVTATLLPFSMFAVREAGAGAPVPMTTLIALLVC
                     LIPTTIGGLLSAIGIAGMDRMIRANVIATSGRAVEAAGDVDTLLLDKTGTITMGNRQA
                     SRLLPAAGVDARHLADAAQLASLADETPEGRSIVVLAKQTFGFRERNIHELGATFIPF
                     SATTRMSGVTIDGREIHKGAADAIGKHLAALGGSLPADVAKAVDDVARQGGTPLVVAE
                     GSRALGVVDLKDVVKGGIRERFDELRRMGIRTVMITGDNPTTAAAIAAEAGVDDFLAE
                     ATPERKLGLIREMQSKGHLVAMCGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDL
                     DSNPTKLIEIVETGKQMLMTRGALTTFSLANDVAKYFAIVPAAFMATYPALGALNVMR
                     LATPQSAILSAVIFNALVIVALIPLALKGVKFRAVGAARLLRDNLLVYGLGGIVAPFV
                     GIKLIDMALTALHLT"
     gene            1586..2161
                     /gene="kdpC"
                     /locus_tag="VF720_00390"
     CDS             1586..2161
                     /gene="kdpC"
                     /locus_tag="VF720_00390"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX7877833.1"
                     /translation="MTALMVSLRFLVAMSLLTGGLYPAVVTLAGRLAFPRAADGSLIR
                     TPDGVAGSELIGRQFDAPGRFWGRPSATAPDPYRADASSGSNLGPSHPDLAAAIEERV
                     ARWRASDPAQTAPIPVDLVTASASGLDPHITPAGALWQVPRVARERGMAPEAVERLVR
                     ARIEPPWFGFLGGARVNVLLLNLDLDRAPPG"
     gene            2184..4793
                     /locus_tag="VF720_00395"
     CDS             2184..4793
                     /locus_tag="VF720_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX7877834.1"
                     /translation="MESSRPTPEAMLDRVREEEARARRGRLKIFFGMAPGVGKTFAML
                     EWAQGRRRDGGDVVIGWLESHGRADTERLSEGLERLPPRVVMHRGLPLSEFDLEGALA
                     RRPQILLVDELPHTNAPGSRHERRWQDVEELVAAGIHVVTTVNVQHIESLGDVVALLT
                     GVVVSETVPDRILDQADEIELVDLPPDDLLQRLREGKVYVPDQAVRAMDGFFTTRNLT
                     ALREMALRRTADRVGARREDGGATGEALMVVVTPAPESANLIRAAYRMASRWRAHWIA
                     LSVETAVFERLSPADRERLAGHLALAQRLGAETLVVRGEKAIDEIRHAARGRRVARLL
                     VGTPDRPGWRERLTGTLAERLVRGGGIEVVAVPGRGGETEPLPATSSRMPLSQYGRAA
                     VVVLGVTLLLGFTRHLLNLADQSMIYLLGVLLVSSTLNRGPSLMAAVLSVAALDFFFV
                     PPYLTFDVAHFRYLLTFAVLLVTAVLVSRQTLLIREQAEAARQRERRTVALYQMTREL
                     ASATEPEPIVEALVRQVRLLLERESILLSNPDDEAPGLVDRDRAVARWVYQHGQPAGF
                     GTRTLPGGEWTFLPAGPEARHGVFGLRMTPGAAPLGPADHQFIEAAIGQTVAALERIS
                     SAEVAEGLRLHVETERTRNLMLSLVSHDLRTPLAAMGGAAETLLASENLPAPERRGLL
                     ESIRREAFRLGRLVGDLLELSRIESGRVPIRRERYPVEELILSALDQTHSLLADRSIR
                     FDRPEELAMVDADGTLLTEALVNLLENAARHTPAGTPVDIRMASSPAGVSIEVADRGP
                     GLPAGEEARIFERFHRGEPAQASGTGLGLALVMAIVKAHGGTVRALNRDGGGAIFRIE
                     LPAAVGGLEAARA"
     gene            4790..5518
                     /locus_tag="VF720_00400"
     CDS             4790..5518
                     /locus_tag="VF720_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006891407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7877835.1"
                     /translation="MNRPSSELPRILLIEDEPEVRRFLRTSLTAQGYQVIEATNGRDG
                     LALAGQHRPVAVLLDLGLPDTDGMAVLVQLREWSSVPVIVVSARGQERQKVEALDAGA
                     DDYLTKPFGLGELLARLRAALRHRPADGDDALADGVADAVRRFGRLTIDTTAHRVLVD
                     EAPVHLTPIEFRILAVLARHAGRVVTQKQLLTAVWGPSGTDRGDSLRVFMTHLRRKLE
                     ADPTAPRLFHTEAGVGYRLIDDEG"
     gene            complement(5552..8380)
                     /locus_tag="VF720_00405"
     CDS             complement(5552..8380)
                     /locus_tag="VF720_00405"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7877836.1"
                     /translation="MRRYLAIRAELAALVLLILPLAPSPAFPAAGDLDPTFSGDGLVQ
                     VGFGQGHDGFNAIAAQADGKTVSVGFGTWASDRDWVIARNEVDGSRDVTFGNFGWVRE
                     SLSTSLDVAYAVLVQPDGKIVVGGESNGQFAVMRLNTDGTPDGTFAVGGLFRHGPAGG
                     GQAWVVRAMALQGTRIILFGKYLPGLTPVAVTMRLNANGTVDGSFGSGGLVTTSILTY
                     GEATSGLVMPDSRILVGGWGADATNRRAVAALRYQANGAPDLTWATTGQVTRYVGTGS
                     SEAFEIAYLTGGTHQTRVALAGRTDGLTGKDALVVMYNDAGAPLGAFGTGGARTYPVA
                     SGDDAFVAMTLIVDALGNPSVACLCGSTRINNSGAFQDALVARIDTMTGTPDVGFDGD
                     GFAIVAYSGGDDSATDIILAAPDKLILGGLYAQAGTKADAFLNRRSRATGANDASYYV
                     NGIWNADYGDAPSRAVEAAVRPDGKIWVAGNHDGDTWDIALVRLHPDGTLDDALSYDG
                     KLVHNGNGHDYVNGMALYPDGRVLVCGSSQFPASDDNFMLARFLEDGSWDPSFDGDGW
                     LVSGMDLSGPANDVLVQPDGRIVGVGYAVAFGVTRARVTRFLDNGAVDITFNGTGHNV
                     RDYDSNYTAWNAVARQSNGKLVMAGFRHIDGIPHLMVGRFNTDGTLDGTFGGGAGYVY
                     PMISPLGSVGQAVAIRPDGRILVTGQSWSGAGSSHTVVRFLSNGSLDTSFDGDGIRIL
                     SLPGGDGVDMDLVLDPAGRMYLAGSQASLPAVTDWSLLRMFHDGTMDTQWSGDGVALV
                     PHQQLYNARAEDVVLDGSGRPIMVGQDGEFMAIRRFDPGIATSSVPEEEGIPSLRVAS
                     PWPNPARLQAAISFELAEAGPVRATIFDVAGRRIRTLADAPRLDPGRHDISWDLRDDT
                     GREVAAGIYLVRVKSEANSSVRRITVAR"
     gene            complement(8478..8777)
                     /locus_tag="VF720_00410"
     CDS             complement(8478..8777)
                     /locus_tag="VF720_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX7877837.1"
                     /translation="MSAYIVVDIDIRDPEAYKEYIRLAPASIRQYGGRYIARGGRTEV
                     FEGKWTPKRLVILEFDTLDRAREWLDSPEYAPARAIRQAAAGADMVAIEGIAVQP"
     gene            complement(8804..10642)
                     /locus_tag="VF720_00415"
     CDS             complement(8804..10642)
                     /locus_tag="VF720_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEX7877838.1"
                     /translation="MLAALGVVFGDIGTSPLYSMRECFHGPHSIEASSENILGVLSLI
                     FWSLVIVISVKYLGFILRADNRGEGGILALSALANRTARGRNTERMVITALGIFGAAL
                     LYGDGVITPAISVLSAVEGLSIVTPVFTKYVIPITCGIIVLIFMVQKNGTGGIGRVFG
                     PVTLLWFLALAILGASWMVRHPSVLEAVNPTHAVDFFVRNGWHGFLVLGSVFLVVTGG
                     EALYADMGHFGARPIRLIWFFLVLPSLLLNYFGQGALLLSDPVHAENPFYHLVPDWAL
                     IPMVILATAATCIASQAVISGAFSLTRQAVQLGYSPRVEIKHTSASEIGQIYVPGVNF
                     ALMVACIWLVLMFKTSSNLAAAYGIAVTATMGITTILFYFVAHEKWRWPNWAALSLCA
                     TFLVIDLAFFGANIVKVADGGWFPLALGLAIFIIMTTWKRGRQILTARHRESEIPQTD
                     FIKMIQDHPPHRVPGTAVFMYRNATGVPSALLHNLKHNRVLHERVVFLTVISEEVPHV
                     AGDERVQVTDLGHGFWRVVIHYGFVEDPDVPKALATIPEETLVVKPMETTYFLGRETI
                     VATKRPGMFIWQERLFSRMIRNERTATSFFKLPPNRVVELGEQIEI"
BASE COUNT         1771 a         3723 c         3911 g         1716 t
ORIGIN      
        1 ggtgcgcgaa tcgggcggcg acttctcggc cgtgaccggc ggcacgcgcg tgctgtccga
       61 ctggctggtc atccgcgtga cggccggagc ggggcagagc ttcctcgacc ggatgattgc
      121 gttggtggaa gggtccaagc gtcgcaagac gcccaacgag atcgcgctgg atatcctgct
      181 ggccggcctc accatcattt tcctggttgt caccgccacc ttgctgccgt tctcgatgtt
      241 cgcggtgcgc gaggcggggg ccggcgcgcc cgtgccgatg acgacgttga tcgcactgct
      301 ggtgtgcctc atcccgacga cgatcggcgg ccttctctcg gccattggca ttgccgggat
      361 ggatcggatg atccgggcga acgtgatcgc cacgtcgggg cgtgccgtcg aggcggccgg
      421 cgacgtggac acgctcctcc tcgacaagac cggcacgatc accatgggga accgccaggc
      481 cagccggctc ctgccggcgg cgggtgtcga cgcgcgtcac ctggccgatg cggcgcaact
      541 ggcctcgctg gccgatgaaa cgccggaagg gcggagcatc gtcgtcctgg ccaagcagac
      601 gttcggcttc cgcgagcgga acatccacga gctgggggcc accttcatcc cgttctcggc
      661 caccacgcgg atgagcggcg tgacgatcga cggccgggaa atccataaag gagcagccga
      721 cgccatcggc aagcacctgg ccgccctcgg cggcagcctg ccggccgacg tggcaaaggc
      781 cgtggatgac gtggcgcggc aggggggaac gccgctcgtg gtggcggagg gatcaagggc
      841 tctgggagtc gtggacctga aggatgtcgt gaagggcggc atccgggagc ggttcgacga
      901 actgcgccgg atgggcatcc ggacggtcat gatcaccggc gataacccga ccaccgcggc
      961 ggccatcgcg gccgaggccg gcgtggacga cttcctggcc gaggccacgc ccgagcggaa
     1021 gctcgggctg atccgcgaga tgcagtcgaa agggcacctg gtggcgatgt gcggcgacgg
     1081 aacgaacgac gcgccggcgc tggcccaggc cgacgtggcc gtggcgatga acagcggcac
     1141 gcaggcggcc aaggaggccg gcaacatggt cgatctcgat tccaatccga ccaagctgat
     1201 cgaaatcgtc gagaccggca agcagatgct catgacccgc ggcgcactca ccaccttcag
     1261 cctggccaac gacgtggcca agtacttcgc gatcgtgccg gcggccttca tggccaccta
     1321 tcccgcgctc ggcgcgctca acgtgatgcg cctggccacg ccgcagagcg ccatcctgtc
     1381 ggcggtcatc ttcaacgcgc tcgtcatcgt ggcgctgatc ccgctggcgc tgaagggggt
     1441 caagttccgg gcggtcggtg ccgcgcggct cctgcgggac aacctcctgg tgtacggcct
     1501 cggcgggatc gtggccccgt tcgtcggtat caagctcatc gacatggcgc tgacggcgct
     1561 gcacctgacg tgaggagttg acctgatgac cgcgctgatg gtctcccttc gattcctcgt
     1621 ggcgatgagc cttctgaccg gcgggctcta cccggccgtc gtcacgctgg ctggacggct
     1681 ggcttttccg cgtgcggcgg atggatcgct catccgcacg ccggacggcg tggcgggctc
     1741 ggagttgatc ggacggcagt tcgatgcgcc gggccgtttc tggggccgcc cgtcggccac
     1801 ggcaccggat ccgtaccggg ccgatgcctc cagcggctcg aacctggggc cgagccatcc
     1861 cgacctggcc gccgcgatcg aggagcgcgt ggcccgctgg cgggcctccg atccggccca
     1921 gaccgcgccg atcccggtgg acctcgtgac ggcgtcggcg agcggcctcg atccgcacat
     1981 cacgccggcg ggggcgttgt ggcaggtgcc gcgcgtggcg cgcgagcggg ggatggcgcc
     2041 ggaagcggtg gaacgactcg tgcgggctcg catcgaaccg ccctggttcg gcttcctggg
     2101 aggcgcacgg gtcaacgtgc tcctcctcaa cctcgacctc gaccgggcgc cgccgggctg
     2161 atggcgggac tatactgacg gccatggaat cgagccggcc gacgcccgaa gcgatgctcg
     2221 atcgggttcg ggaggaggaa gcccgtgccc gccgggggcg actcaagatc ttcttcggca
     2281 tggcgcccgg tgtcgggaag acgttcgcca tgctggaatg ggcgcagggg cgtcggcgcg
     2341 acggcgggga tgtcgtcatc ggctggctgg aatcgcacgg tcgggccgac accgagcgcc
     2401 tgtccgaggg gctcgagcgg ctgccacccc gcgtcgtgat gcatcgcggc ctgcctctgt
     2461 cggaattcga tctcgagggg gcgctggcgc gccggccaca gattctcctg gtcgacgagt
     2521 tgccccacac caacgccccg ggatcccggc acgaacgtcg gtggcaggac gtggaggagc
     2581 tggtggcggc cggcatccat gtggtcacca ccgtcaacgt ccagcacatc gagagcctgg
     2641 gcgatgtcgt ggccctgctc accggagtgg tcgtctcgga aaccgttccg gatcgcatcc
     2701 tcgaccaggc ggacgagatc gagctggtcg acctgccgcc ggacgacctg ctccagcggc
     2761 tgcgggaggg gaaggtgtac gtgccggacc aggcggtccg ggccatggat ggcttcttca
     2821 cgacccgcaa tctcactgcg ctgcgcgaga tggccctgcg ccgcaccgcc gaccgggtgg
     2881 gcgcgcgccg ggaagatggc ggggccaccg gcgaggcgct catggtggtt gtgacgcccg
     2941 cgcccgagtc ggccaacctg atccgtgccg cctacaggat ggccagccgg tggcgggcgc
     3001 actggatcgc cctgtcggtg gagaccgctg tcttcgaacg cctctcgccc gccgaccgcg
     3061 agcggctggc cggtcacctg gcgctggccc agcggctcgg cgccgaaacg ctggtggtgc
     3121 gtggcgagaa ggccatcgac gaaatccgtc acgcggcgcg cggccggcgg gtggcgcgcc
     3181 tccttgtcgg cacgccggat cggcccggct ggcgcgaacg gctcaccggc acgctggccg
     3241 agcgactggt gaggggcggc ggcatcgagg tcgtcgcggt tcccggccgc ggaggcgaaa
     3301 cggagccgct gccggccaca tcttcgagaa tgccgctctc acagtacggt cgcgcggccg
     3361 tggtggtgct cggcgtcacg ctcctcctgg ggttcacgcg ccatctcctg aacctggccg
     3421 atcagtcgat gatctacctg ctcggcgtcc tgctcgtctc cagcacgctg aaccgcggtc
     3481 cctcactgat ggccgcggtc ctgagcgtgg cggcgctcga ctttttcttc gtgccgccgt
     3541 acctcacgtt cgacgtggcc cacttccgct atctcctcac gttcgcggtt ctgctcgtca
     3601 cggccgtgct ggtcagccgg cagacgctcc tcatccgcga gcaggccgag gccgcccgcc
     3661 aacgggaacg acgcacggtg gcgctctacc agatgacgcg ggagctggct tcggccaccg
     3721 agcccgaacc gatcgtcgag gcgctcgtgc gacaggtgcg gctgctgctg gaacgtgagt
     3781 cgatccttct ctccaatccg gatgacgagg ctccgggcct ggttgatcgt gatcgggccg
     3841 tggcgcgctg ggtgtaccag cacgggcagc cggccgggtt cggcacgcgc acgcttccgg
     3901 gcggcgaatg gacgtttctt ccggcggggc ccgaggcgcg gcacggtgtg ttcggcctca
     3961 ggatgacgcc gggcgccgcc ccactcgggc ccgcggatca ccagttcatc gaggccgcga
     4021 tcggccagac cgtggcggcg ctggaacgga taagctcggc cgaggtggcc gaggggctcc
     4081 ggctgcacgt ggaaaccgag cggacgcgca atctgatgct cagcctcgtc tctcacgatc
     4141 tgcgcacgcc gctcgcggcg atggggggcg cggccgaaac gttgctggcc agcgagaacc
     4201 tcccggcgcc ggaacgccgt gggctcctgg agtcgatccg gcgagaggca ttccggctcg
     4261 ggcgcctggt gggcgatctc ctcgagctga gccggatcga atcggggcgg gtgccaatcc
     4321 gacgcgagcg ctacccggtg gaggagttga ttctctcggc tctcgaccag acacattcac
     4381 tgctggccga tcgatcgata cgcttcgacc gaccggagga actcgccatg gtggatgccg
     4441 acggcaccct tctcaccgag gccctcgtca acctgctcga gaacgcggca cgacacaccc
     4501 cggctggcac gccggtcgac atccggatgg cgtcgtcgcc ggcgggcgtg agtatcgagg
     4561 tggccgatcg cgggccgggg ctgccggccg gcgaggaggc gcggatcttc gaacgcttcc
     4621 atcgcggcga gccggcccag gcttccggca cggggctggg cctcgcactc gtgatggcca
     4681 tcgtgaaggc gcacggcggc acggtccggg ccctcaatcg tgacggcggc ggcgccatct
     4741 tccgtatcga actgccggcg gccgtcggcg gcctggaggc ggcccgcgcg tgaaccggcc
     4801 gtcgtccgaa cttccgcgca tcctcctcat cgaggatgaa cccgaggtgc gccgcttcct
     4861 ccgcaccagt ctcaccgcgc agggttacca ggtgatcgag gccaccaatg gccgggacgg
     4921 gcttgccctg gccggtcagc accggccggt ggcggtcctg ctcgacctgg gtctgccgga
     4981 cacggatgga atggcggtcc tcgtccaact ccgcgagtgg tcgagcgttc cggtcatcgt
     5041 ggtgtcggcc cgcgggcagg agcggcagaa ggtggaagcg ctcgacgccg gcgcggatga
     5101 ctatctcacc aagccgttcg gcctgggcga actgctggcg cggttgcggg cggccttgag
     5161 acatcggccc gcggacggcg atgacgccct cgccgacggc gtcgccgacg cggtccgtcg
     5221 gttcggccgg ctgacgatcg acacgaccgc tcaccgcgtc ctggtggacg aagccccggt
     5281 gcacctcacg ccgatcgagt tccgcatcct ggccgtgctg gcgcgccacg ccggtcgcgt
     5341 cgtcacccag aaacaactcc tcactgcggt gtggggcccc tccggcaccg accgcggcga
     5401 ttcgctccgc gtcttcatga cccacctgcg tcgcaagctc gaagccgatc ccacggcgcc
     5461 ccgcctcttc cacaccgagg cgggcgtcgg gtaccggttg atcgatgacg aggggtgacg
     5521 ggagccacgt cgggaagcgc cgccgcgttg gtcagcgggc cacggtgatt cgtcggacgg
     5581 aggagttggc ctccgatttc acccgcacca ggtagatgcc ggcggcaacc tcccggccgg
     5641 tatcgtcccg caggtcccag ctgatgtcgt gccgtcccgg gtcgaggcgc ggcgcatcgg
     5701 caagcgtccg aatacgccgg cccgccacgt cgaagatggt ggcgcgcacg gggcccgcct
     5761 cggcgagctc gaacgagatg gcggcctgga gccgggccgg gttgggccac gggctcgcca
     5821 cgcggagcga gggtatcccc tcctcttccg gaacggacga ggtggcgatg ccgggatcga
     5881 agcggcggat cgccatgaat tccccatcct ggccgaccat gatcggcctg ccgcttccat
     5941 cgaggaccac gtcctcggcg cgggcattgt agagctgctg gtggggaacg agggccacgc
     6001 cgtcgccgga ccactgcgtg tccatcgtgc cgtcgtggaa catccggagg agcgaccagt
     6061 cggtgacggc cgggaggctc gcctggctcc ccgcgaggta catgcggccg gccggatcga
     6121 ggaccaggtc catgtcgacg ccgtcgcctc cgggcaggct caggatgcgg atcccgtcgc
     6181 cgtcgaagga ggtgtcgagc gagccgttgg agaggaagcg gacgaccgtg tgggatgatc
     6241 ccgcgcccga ccatgactga cccgttacca ggatgcgccc gtccggccgg atggccacgg
     6301 cctggccgac gctgccgagg gggctgatca tcgggtagac gtaaccagcg ccgccgccaa
     6361 aggtgccgtc gagcgtgccg tcggtgttga aacgccccac catcaggtgg ggaatgccgt
     6421 cgatgtgtcg gaaaccggcc atcacgagct tgccgttgga ctgacgggcc accgcgttcc
     6481 acgccgtgta gttcgagtcg tagtcgcgca cgttgtggcc ggtgccgttg aaggtgatgt
     6541 cgacggcgcc gttgtccagg aagcgcgtga cgcgcgcccg ggtcacgccg aacgcgacgg
     6601 cgtagccgac gccgacgatc cggccgtcgg gttgcaccag gacgtcgttg gccggaccgg
     6661 aaaggtccat gccggacacc agccagccgt cgccgtcgaa cgagggatcc cagctgccgt
     6721 cctcgaggaa acgggccagc atgaagttgt cgtccgacgc cgggaactgg ctcgagccgc
     6781 atacgaggac gcggccgtcg gggtaaaggg ccatgccgtt cacatagtcg tggccgtttc
     6841 cgttgtggac cagcttgccg tcgtaactca gggcgtcgtc gagggtgccg tcaggatgga
     6901 ggcgcacgag cgcgatgtcc catgtgtctc cgtcatggtt gccggccacc cagatcttcc
     6961 cgtcggggcg caccgcggcc tcgacggcgc gcgacggcgc atcaccgtag tcggcgttcc
     7021 agatgccgtt cacgtagtag ctggcgtcgt tggcgccggt agcgcgcgag cgacggttca
     7081 ggaacgcgtc ggccttggtg ccggcctggg cgtagagtcc gcccaggatc agcttgtccg
     7141 gcgccgccag gatgatgtcg gtcgccgagt catccccgcc cgagtaggcc acgatggcaa
     7201 acccgtcgcc gtcgaagccg acgtccggcg tgccggtcat ggtgtcgatg cgggccacca
     7261 gcgcatcctg gaaggcgccg ctgttgttga tgcgcgtgct cccgcacagg caggccaccg
     7321 acgggttgcc aagggcgtcg acgatcagcg tcatcgccac gaaggcgtca tcgccggagg
     7381 ccaccgggta ggtccgcgct ccccccgtgc cgaaggcgcc gaggggcgcg cccgcgtcgt
     7441 tgtacatgac caccagcgca tccttgccgg tgagaccgtc cgtccgtccg gccagcgcca
     7501 cccgcgtctg atgcgttccg ccggtgagat aggcgatctc gaacgcctcg ctgcttcccg
     7561 tgccgacata ccgcgtcacc tggccggtcg tggcccaggt gaggtcgggc gcgccgttgg
     7621 cctggtaacg caacgccgcc accgcgcgcc ggttcgtggc gtcggcgccc cagccgccca
     7681 ccaaaatccg gctgtcaggc atgacgaggc cggacgtggc ttcgccgtac gtcaggatcg
     7741 acgtcgtgac caggccgccg ctcccgaacg agccgtccac cgtgccgttg gcgttcaatc
     7801 gcatcgtcac ggccaccggc gtgagcccgg ggagatactt gccgaacagg atgatccgcg
     7861 tcccctgcag cgccatcgcc cgcaccaccc atgcctgccc gccgccggcc ggaccgtgac
     7921 ggaagaggcc gccgacggca aaggtgccat ccggcgtgcc gtcggtgttc aatcgcatga
     7981 ccgcgaactg cccgttcgat tcgcccccga ccacgatctt cccgtccggc tggacgagca
     8041 cggcgtacgc cacgtcgagg ctggtggaca gggactcccg gacccagccg aagttgccga
     8101 acgtcacgtc acgcgagccg tccacctcgt tccgggcgat cacccagtcg cgatccgaag
     8161 cccacgtgcc gaagccgacc gaaaccgtct tgccgtccgc ctgggcggcg atggcattga
     8221 agccgtcatg cccctgcccg aaaccgacct ggacgaggcc atcgccgctg aacgtggggt
     8281 cgaggtcgcc ggcggcgggg aaggcggggg aaggggccag tggaaggatc aggagcacaa
     8341 gggcggcaag ctcagcccgg atagcaagat agcggcgcat ggactcctcc aggggcgaca
     8401 ggcggtgatg gtcaccggag gaagcgccga atggcgggaa agtggctcac gcggacggcg
     8461 gggaatgagc tagccgctca gggctgcacg gcaatcccct cgatcgccac catgtcggct
     8521 ccggccgctg cctggcgaat ggcccgcgcc ggcgcgtact ccggcgagtc gagccactcg
     8581 cgtgcccggt cgagggtatc gaactcgagg atgaccaggc gcttcggcgt ccactttccc
     8641 tcgaacacct cggtgcgccc gccacgcgcg atgtacctgc cgccgtactg cctgatcgag
     8701 gcgggcgcga ggcggatgta ctccttgtag gcttcgggat ccctgatgtc gatgtcgacg
     8761 acgatgtagg cgctcatgcg atctccttgg gcgccgtccg ggatcagatc tcgatctgct
     8821 cgccgagctc cacgacgcgg ttcgggggca acttgaagaa cgacgtggcc gtccgttcgt
     8881 tgcggatcat gcgggagaag aggcgctcct gccagatgaa catgccgggg cgcttggtgg
     8941 ccacgatcgt ctcgcgaccg aggaagtagg tggtttccat cggcttcacg accagcgttt
     9001 cctcaggaat cgtggccagg gccttcggca cgtcgggatc ctccacgaaa ccgtagtgga
     9061 tgaccacccg ccagaaacca tgccccaggt cggtcacctg gacgcgttcg tcgccagcca
     9121 cgtgcggaac ttcttcggag atcacggtca ggaacacaac ccgctcatga agcacgcggt
     9181 tgtgcttcag attgtgcagc agcgccgacg gcacgccggt ggcattgcgg tacatgaata
     9241 cggcggttcc tggaacccgg tgcggcgggt gatcctggat catcttgatg aagtctgtct
     9301 gcgggatctc ggactcacga tgccgcgccg tcaggatctg ccttccgcgt ttccaggtgg
     9361 tcatgatgat gaagatggcg aggccgagtg ccagcgggaa ccaccctccg tcggccacct
     9421 tcacgatgtt ggcgccgaag aacgccaggt cgatgaccag gaatgtcgcg cagagcgaga
     9481 gcgccgccca gttgggccag cgccacttct catgggccac gaagtagaac aggatggttg
     9541 tgattcccat ggtggcggtc accgcgatgc cgtaggcggc ggccaggttg ctggacgtct
     9601 tgaacatcag caccagccag atgcaggcca ccatgagcgc gaagttcaca cccggcacgt
     9661 agatctggcc gatttcgctg gccgaggtgt gcttgatctc cacgcggggc gagtagccga
     9721 gctgcaccgc ctggcgggtc aggctgaacg cccccgagat gaccgcctgg ctggcgatgc
     9781 aggtggcggc cgtggccagg atcaccatcg ggatgagcgc ccagtcgggc accaggtgat
     9841 agaacggatt ctccgcgtgc accgggtcgg agaggagaag cgcgccctgc ccgaaatagt
     9901 tgaggaggag cgacggcagg accaggaaga accagatcag ccggatcggc cgcgcgccga
     9961 agtgccccat gtcggcgtag agtgcctcac cgccggtcac cacgagaaac acggagccga
    10021 gtaccaggaa accgtgccag ccgttccgca cgaagaagtc caccgcgtgc gtcggattca
    10081 ccgcttcgag taccgacggg tgccgcacca tccacgacgc gccgaggatg gcgagcgcca
    10141 ggaaccagag aagcgtgacc gggccaaaga cgcggccgat accgcccgtg ccgttcttct
    10201 gcaccatgaa gatcagcacg atgatcccgc aggtgatcgg gatgacatac ttcgtgaaga
    10261 ccggtgtcac gatggagagc ccctccaccg ccgataaaac cgaaatggcc ggcgtgatga
    10321 cgccgtcgcc gtacaggagc gcggcgccga agatgccgag tgccgtgatc accatccgct
    10381 cggtattccg cccccgagcg gtccggttgg ccagcgccga aagcgccagg atccccccct
    10441 cgccccggtt gtccgcgcgc aggatgaatc ccaggtactt gaccgagatg acaatgacca
    10501 gcgaccagaa gatgagcgac agaacgccga ggatgttttc cgacgaagct tcgatcgaat
    10561 gcggcccatg aaaacattcc cgcatcgagt agagggggct cgtcccgatg tccccgaaaa
    10621 cgacgcccag ggccgccagc atcaggagat agaggtaact gccctgcggg gcggcggggt
    10681 ggtggcggcg atgatgtcgt gaagcggcgg atccggccaa gcgcgtgtcc ttgaaacccg
    10741 ctcctcggga gcggaccggc cgacccagcc gggtcggcca agcggcggat attacgccgg
    10801 gggtacgggg ggggcaaggc gggcgggaag tccccgcgcg ccgtgaaaag tggcccagga
    10861 catgcctggt tccgatgcct cctctggaca ttcccgcacc gcatggcgaa tatccccggt
    10921 ctgaccaacc gagccattcc gcctcccgat gaggacccca tgccgaactc ccgcaagaag
    10981 ccagtcaaac gttcgggccg caaggcggcc cgtcgaccga tcacgcccga agacctgacg
    11041 cgtttcgtct tcgtttcgga tccgcaggtg tcgccggatg gatcgcgcgt ggtgttcgtg
    11101 cggaagcacg tgggggagaa g
//
LOCUS       DASVOD010000022         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_157731, whole genome shotgun sequence.
ACCESSION   DASVOD010000022 DASVOD010000000
VERSION     DASVOD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157731"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..>1725
                     /gene="acs"
                     /locus_tag="VF720_00420"
     CDS             141..>1725
                     /gene="acs"
                     /locus_tag="VF720_00420"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX7877839.1"
                     /translation="MAEAVYPVPAEWSRNALIDDARYQEMYERSVSEPEAFWREEARR
                     IDWIKPFSVVKQTSFHEEDFGISWFTDGTLNLSANCLDRHLVERGDQIAILWEPDSPT
                     APDRRITYRQLHEQVCRLANLLKARGVRKGERVTLYLPMVPEAAVAMLACARIGAIHS
                     IVFAGFSPDALAGRIQDCDSRVVLTADEGLRGGKKVPLKANVDEALKACPGVDSVIVL
                     AHTKSGVPMAEGRDIDWASAVADRAAECEPVEMSAEDPLFILYTSGSTGKPKGVLHTT
                     GGYSVWASMTHEYVFDYRPGQIYWCAADIGWVTGHSYVVYGPLMNGATTVMFEGVPNF
                     PDPSRFWQVVDKFGVEIFYGAPTALRALMREGDDWVKKTSRQSLRLLGSVGEPINPEA
                     WSWYHEVVGEGRCPIVDTWWQTETGGVMISPLPGATPLKPGSATRPMIGVQPKMLDND
                     GKEIEGPGDGCLVITDSWPGQMRTVWGDHERFFQTYFSTFKGTYFTGDGCRRDEDGYY
                     WITGRIDDVINVSGHRMGTA"
BASE COUNT          309 a          580 c          544 g          292 t
ORIGIN      
        1 cccgccatct tcgtcgaaac ggcacgagat tccgcccggc ccgttctacc attgtccaat
       61 gggaacggcc cgggtccatt gtgctatgca acatgcaagg ccgggagagc ccgaccctac
      121 gatatgagag gagaatccgc atggccgagg ccgtctatcc cgttcccgcc gagtggagcc
      181 ggaacgccct gatcgacgat gcccgctatc aggagatgta cgaaaggtcg gtttccgagc
      241 ccgaagcatt ctggcgcgag gaagcgcggc ggatagactg gatcaagccc ttcagcgtgg
      301 tcaagcagac cagtttccac gaagaggact tcggcatttc gtggttcacc gacggcaccc
      361 tgaacctctc ggccaactgc ctcgaccgtc atctcgtgga gcgtggcgac cagatcgcga
      421 tcctgtggga gccggacagt cccaccgctc ccgaccgccg catcacttac cgccagttgc
      481 acgagcaggt ctgccgcctg gccaatctcc tcaaggcacg cggcgtccgg aagggtgagc
      541 gcgtcactct ctacctgccg atggtgccgg aagcggccgt tgccatgctg gcctgcgcgc
      601 ggatcggcgc aatccactcg atcgtcttcg ccgggttctc gccggacgcg ctggcgggcc
      661 gcatccagga ttgcgacagc cgcgtcgtcc tcactgccga cgagggcctg cgtgggggta
      721 agaaagtccc gctcaaggcc aatgtcgatg aggcgctcaa ggcctgtccc ggtgtcgaca
      781 gcgtgatcgt cctcgcgcac accaagtccg gcgtaccgat ggccgagggt cgcgacatcg
      841 actgggcctc ggccgtcgcc gatcgggccg ccgaatgcga gccggtcgaa atgagcgcgg
      901 aggacccgct gttcatcctc tacacttcgg gctccactgg aaagcccaag ggcgtgttgc
      961 acaccaccgg cggctattcg gtatgggcct cgatgaccca cgagtacgtc ttcgactacc
     1021 gccccggcca gatctactgg tgcgcggccg acatcgggtg ggtcaccggc cacagctacg
     1081 tcgtctacgg cccgctgatg aacggtgcga ccaccgtgat gttcgaaggc gtgcccaact
     1141 tccccgatcc cagccgtttc tggcaggtcg tcgacaagtt cggcgtcgag attttctacg
     1201 gtgcccccac cgcgctgcgt gcgctcatgc gcgaaggcga cgactgggtg aagaagacca
     1261 gccgccagtc gctgcgcctc ctcggctcgg tcggcgaacc aatcaacccg gaggcatggt
     1321 cctggtatca cgaggtcgtg ggcgaggggc gttgcccgat cgtcgatact tggtggcaga
     1381 ccgagaccgg cggcgtgatg atctccccgt tgccgggcgc caccccgctc aagcccggct
     1441 cagccacccg cccgatgatc ggcgtgcagc cgaaaatgct cgacaacgac ggcaaggaaa
     1501 tcgaggggcc gggcgacggc tgcctcgtca tcacagacag ctggcccggc cagatgcgca
     1561 ccgtctgggg cgatcatgag cgcttcttcc agacctattt cagcaccttc aagggcacgt
     1621 acttcaccgg tgacggctgc cgccgggacg aggacggcta ctactggatc acgggccgca
     1681 tcgacgatgt catcaacgtc tccgggcatc gcatgggcac cgcgg
//
LOCUS       DASVOD010000023         6903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_783775, whole genome shotgun sequence.
ACCESSION   DASVOD010000023 DASVOD010000000
VERSION     DASVOD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783775"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..231
                     /locus_tag="VF720_00425"
     CDS             67..231
                     /locus_tag="VF720_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020025523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877840.1"
                     /translation="MSIGVMTCGKCNKDLKHSTLTKADGTTVHISECPEGHGKVKSPM
                     CCGQDMSCQV"
     gene            257..2293
                     /locus_tag="VF720_00430"
     CDS             257..2293
                     /locus_tag="VF720_00430"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7877841.1"
                     /translation="MMRANPMIAGLALLGTLFLPAFGRAESLSPMSSGVDLSAIDRSV
                     RPQDNFYQFANGAWLDRTEIPAIYSSYSVYQEVNERVEVALQEIIEEAAAHPGPAGSE
                     SRMVADLFTTWMDVDTINRLGVSAVKPELAIVDGITDKKSLVEAFGRFTRIGVGGPIR
                     FNIDQDARNSTVYAVYVNQGGLTLPDRDFYLDTTNPKFDKARQALPGYIASMLKAAGR
                     DGAGAPGIVELETAIARAQWDKVKNRDNEATYNKRTLAEMAALAPHVDWQAWLSVLGA
                     GSVDHVIVGQPDFFTALDGMIESTSLDTWKNYLAYRVMDARAGDMDSTTAAIRFEFRN
                     RTLNGQKEERERWKRGVSLVDRMVGEAVGKLYVAKYFPPEAKTRMVAMVQNVVKTLDQ
                     SLAELDWMTPATRVKAREKLARFTPKIGYPDVWLDYTGMQIVPGDHVGNVRRAIEWDH
                     NRQVNKLGKPVDRTEWGMTPQTVNAYYNASLNEVVFPAARLQPPFFQLEADDAINYGA
                     VGGVIGHEISHGFDDQGSGFDGDGNMSNWWTPEDKTAFEARTKALIAQYDAFSPIEGM
                     HVNGAFTLGENIGDLSGVAMAYRAYIASLNGKEAPVIDGFTGPQRFFIGYAMSRKGKM
                     RDEQTIQRLASDPHSPLEFRVTGPYRNIDAFHEAFGTKPGDGMWLAPEERVRLW"
     gene            complement(2319..3140)
                     /locus_tag="VF720_00435"
     CDS             complement(2319..3140)
                     /locus_tag="VF720_00435"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX7877842.1"
                     /translation="MPLTAFQLRIARILAVNRSPDSHLAGGAALHFAPNSKRYSNDLD
                     YFHDSVERVATAFGSDRDELKKHGITVELEMNQPGYLRAVVSSGNDQTKIEWAHDSSW
                     RFMPAIYNSDVGFQLHPIDIAVNKVMALAGRDEARDFVDILYIDKEILPLGPLCWAAA
                     GKDPGLSPGSLLELLKRRGRYRPEDFSALMMTEPMNLAEMKSEWLEILNSADNFIRGA
                     PADQIGCLYYSKRLQTFVDPQAHGDTTDIELHFGRPGGILPKFYPGDPFPRTKDS"
     gene            complement(3131..3397)
                     /locus_tag="VF720_00440"
     CDS             complement(3131..3397)
                     /locus_tag="VF720_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877843.1"
                     /translation="MSTSLRKHLARIGRRGGLKSRRILAPEMAREMVRIREARRAYRR
                     FHTSCFWSYDPDHRIMVADIPWVIEGLRKNGNREAWELASRLCH"
     gene            complement(3530..6511)
                     /locus_tag="VF720_00445"
     CDS             complement(3530..6511)
                     /locus_tag="VF720_00445"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX7877844.1"
                     /translation="MSWDWQRYVMTATQPRVRVPVIFGGLSWIAGVALMCLATPGFVA
                     SGYAETPVLDATVPGAARFQNYDSPPGSGDDSGEPSIGSNWTTEQSFSNSHGAIPNGG
                     TTTYFGGFSPYMLRITFNDCQSPAIATWEEKSLLTASTPRVFGDPILFTDRVTGRTFV
                     SQEEGLTPLGSTTDFTDDDGDTFLPTQGSGVPSCVDHQTIGGGPFHAPLTGIGYPHAV
                     YYASQCVGDAVCSLSLDGGVTFLPSVPMYTVADCAGLHGHIKVGPDGTVYIPNKGCGG
                     TLPFHDGGQAAVVVSEDNGVTWDVRPIPGSVGMIDDDPSVGVATNGNIYLGWQSSDGH
                     PRIAKSTDKGLTWGPLRDVGTSLGIVNCVFPEVVAGDGDRAAFAFYGTTTAGAYDQPD
                     FPGVWYLYIATTYDGGNTWTTVNATPGDPIQRGGVCDDGDCRNMLDFFDITVDKEGRI
                     LVGWDDGCIGGCVTGGGNSFSAKAVITRQSGGKRLFAAYDPVEPGVPQAAGLTAGIVG
                     PVVHLAWTAPDNGGATITEYRVYHSSTGDAGPFSVVGTVNETAFDHVVNPANQNRYRV
                     TAVNSMGEGPYCQTVAPNSGPVANACIVPGLRSVSDVNSDGSDNDSGQNTPVDPAVNI
                     RALSVSEPYLGPGVAQLTFTLQVEPTTGSIPSSSQWYIVWNRATVAADGSDRRFVAMK
                     SNASGALSFVYGDFGPPLPLDGSLPALNANTPTVLGNADSGTYDPATGLITIKLAANK
                     ADAPVALGAGDDLAGLNVRTFLLKPDGGPRSQNIASDITGDGNYVLVGNASCFVNSAP
                     IASLDATPTEGHAPLIVTFDASGSSDPDPGDGVAMYTFNFGDGSSPVTQSSATINHTY
                     NSASGPSGFFATLTVEDQAGKGSVNVASIHIQVTPAISGVNPVVLPTRFNLAPMTNPA
                     QDQMFLTLDVKEPGPVTVQVFSATGRLVANLLQSPMSAGTHSLHWRAIDSSGAPVPAG
                     MYMVRARTGEEVTTARVVLVH"
BASE COUNT         1223 a         2107 c         2358 g         1215 t
ORIGIN      
        1 cccgtcccga aaaacccctt tccaaaggag ccccccatgg cggtctacgt ctgtgagaag
       61 tgtggcatga gcattggtgt catgacgtgc ggcaagtgca acaaggatct gaagcacagc
      121 acgttgacga aggcggatgg cacgacggtg catatttcgg aatgtccgga gggacacggc
      181 aaggtgaagt cgccgatgtg ctgcgggcag gacatgagct gccaggttta ggcggcggtt
      241 ggaactggag gcaccgatga tgcgcgcgaa cccgatgatc gctggcctgg ctctgctggg
      301 caccctgttc ttgccggcct tcggccgggc ggaatctctg tcaccgatgt cgtccggtgt
      361 tgatctcagc gccatcgatc ggtcggtgag gccgcaggac aacttctacc agtttgccaa
      421 tggggcgtgg ctcgacagga cggagattcc ggccatctac tcgagctact cggtttacca
      481 ggaggtgaac gagcgggtgg aggtggcgct gcaggagatc atcgaggagg cggcggcgca
      541 tccggggccg gcggggtcgg agtcgcggat ggtggcggat cttttcacga cctggatgga
      601 cgtggacacg atcaaccggc tgggcgtgag cgcggtgaag ccggaactgg ccatcgttga
      661 cggcatcacc gacaagaagt cgctggtgga ggcgttcggg cgtttcaccc gcatcggtgt
      721 gggcgggccg atccgcttca acatcgacca ggacgcgcgc aattccacgg tgtatgcggt
      781 ctacgtgaac cagggggggc tgacgcttcc ggatcgcgat ttctacctcg acacgacgaa
      841 tccgaagttc gacaaggcgc ggcaggcgct accgggttac atcgcttcga tgctgaaggc
      901 cgccgggcgc gatggcgccg gggccccggg catcgtggag ctggagacgg ccatcgcccg
      961 ggcgcagtgg gacaaggtca agaaccggga caacgaggcc acctacaaca agcgcacgct
     1021 ggccgagatg gcggccctgg cgccgcatgt ggactggcag gcctggttga gcgtgctggg
     1081 cgcgggttcc gtggatcacg tgatcgtcgg tcagccggat ttcttcacgg cgctcgacgg
     1141 gatgatcgag tcgacttccc tcgacacctg gaagaactac ctcgcctacc gggtgatgga
     1201 tgcccgcgcc ggcgacatgg attcgacgac ggccgccatc cggttcgagt tccgtaatcg
     1261 cacgctcaat ggacagaagg aagagcgcga gcggtggaag cgcggtgtct cgctcgtcga
     1321 ccgcatggtg ggggaggcgg tgggcaagct ctacgtggcc aagtacttcc cgcccgaggc
     1381 caagacgcgc atggtggcca tggtgcagaa cgtcgtgaag acgctggacc agagcctggc
     1441 cgagctcgac tggatgacgc ccgccacccg cgtgaaggcc cgtgagaagc tggcccgctt
     1501 cacgcccaag atcggctatc ccgacgtgtg gctcgactac accggcatgc agatcgtgcc
     1561 gggcgatcat gtcggcaacg tgcggcgcgc catcgagtgg gaccacaacc gccaggtgaa
     1621 caagctcggc aagccggttg accgcaccga gtgggggatg acgccccaga cggtcaacgc
     1681 ctactacaac gccagcctca acgaggtggt cttcccggcg gcgcgcctgc agccaccgtt
     1741 ctttcagctc gaagcagacg atgccatcaa ctacggtgcg gtgggcggcg tcatcggcca
     1801 cgaaatctcc cacggcttcg acgaccaggg gagcggtttc gacggcgacg gcaacatgtc
     1861 gaactggtgg acaccggaag acaagacggc cttcgaggcg cgcaccaagg cgctcatcgc
     1921 ccagtacgac gcgttctcgc cgatcgaggg aatgcacgtg aacggcgcct tcacgctcgg
     1981 cgaaaatatc ggcgacctgt cgggcgtggc gatggcctac cgggcctata tcgccagcct
     2041 gaacgggaag gaagcgccgg tcatcgatgg cttcaccggg ccgcagcgct tcttcatcgg
     2101 ctacgcgatg tcacggaaag ggaaaatgcg ggacgagcag acgatccagc gcctggcttc
     2161 cgacccgcat tctcccctcg aattccgtgt taccgggccc taccggaaca tcgatgcctt
     2221 ccacgaggcc ttcggcacca agccgggtga cggcatgtgg ctggcgccgg aggaacgcgt
     2281 ccggctctgg taaatcgatt gggcggccgg cactgaattc aggaatcctt cgttcgagga
     2341 aatggatcgc ctgggtagaa cttcggcaga atccccccag gcctgccgaa gtggagctca
     2401 atgtcagtgg tgtctccatg cgcttgcgga tccacaaatg tttgcaggcg cttcgagtaa
     2461 tagaggcatc cgatctggtc ggcgggggcg ccccggataa agttgtccgc ggaattcaat
     2521 atttcgagcc actctgactt catttcggcg agattcatcg gctccgtcat catcagcgcc
     2581 gaaaagtctt caggccggta ccgaccgcgc cgtttcagga gctccagcaa ggatcccggg
     2641 ctgaggccgg gatcctttcc cgctgccgcc cagcataagg ggcctaaagg aagaatttcc
     2701 ttgtcgatgt acaggatatc gacgaagtcg cgtgcctcgt cccggccggc gagcgccatt
     2761 actttgttca ccgcgatgtc gatcggatgc agctggaatc ccacgtcgga attgtagatg
     2821 gccggcatga atcgccaact tgagtcgtgg gcccattcaa tcttcgtttg atcgttgcca
     2881 ctggacacga cggcgcgcaa atagcctggc tggttcatct ccagttcgac ggttatgccg
     2941 tgttttttca gttcgtcgcg gtcgctcccg aacgccgtgg ccactcgctc gaccgagtca
     3001 tgaaagtagt cgaggtcatt gctgtatcgc ttcgagttcg gcgcgaagtg aagtgcggca
     3061 cctcccgcca gatgactgtc gggggagcgg ttgacggcca ggatgcgagc gatcctcagc
     3121 tgaaaagcgg tcagtggcat agtcggctcg ccagttccca ggcctcgcga tttccgtttt
     3181 ttcggagccc ttcgatcacc cacgggatgt cggcaaccat gatccggtga tcaggatcgt
     3241 aggaccagaa gcatgaggtg tgaaaacgcc ggtaggcccg gcgagcttcg cggatacgaa
     3301 ccatctcccg ggccatttcg ggtgccagga tgcggcggct cttgagcccg ccccggcggc
     3361 cgatccgcgc gaggtgtttc cgaagtgaag ttgacatgta tctatcgtaa agggcttatg
     3421 atgttaagtc aattaatata atgaattaca acgacagaaa tgcagaaagg gcgacgctcg
     3481 aaagtcaacg ccggtccggc cgcagccgaa ccggcgttgg cgaatccagc tagtgcacca
     3541 gcacgacccg ggccgtcgtc acttcctcgc cggtcctggc ccgcaccatg tacatgccgg
     3601 ccggcaccgg ggcgccgctc gagtcgatgg cccgccagtg cagcgaatgc gtgccggccg
     3661 acatcggtga ctggagcagg ttcgcgacga ggcggccggt cgcgctgaac acctgcaccg
     3721 tcaccgggcc gggttccttc acgtccagcg tcaggaacat ctggtcctgg gcaggattgg
     3781 tcatcggggc caggttgaac cgcgtgggca gcaccaccgg gttcacaccc gaaatggccg
     3841 gggtcacctg gatgtggatc gaggccacgt tcacgctccc cttgccggcc tggtcttcca
     3901 ccgtgagcgt ggcgaagaag ccgctcgggc cgctggccga gttgtaggtg tggttgatgg
     3961 tggcgctcga ctgggtgacc ggcgaggagc cgtcgccgaa gttgaacgtg tacatcgcca
     4021 cgccgtcacc gggatcggga tccgacgagc cggaggcatc gaaggtcacg atcagcgggg
     4081 cgtgtccctc ggtgggcgtg gcatccaggc tggcgatcgg cgccgagttc acgaagcacg
     4141 aggcgttccc caccagcacg tagttgccgt cgcccgtgat gtccgaggcg atgttctgcg
     4201 agcgcggtcc gccatccggc ttgagcagga aggtgcggac gttgagcccg gccaggtcgt
     4261 cgccggcacc gagcgccacg ggcgcgtcgg ccttgttggc cgcaagcttg atggtgatga
     4321 ggccggtggc cggatcgtag gtgccgctgt cggcgttccc gagcacggtg ggggtgttgg
     4381 cgttgagtgc gggaagggag ccgtcgaggg ggagcggcgg gccgaaatcg ccgtacacga
     4441 acgacagggc tccggacgcg ttgctcttca tcgccacgaa gcgacggtca ctgccgtcgg
     4501 cggccacggt ggcccggttc cagacgatgt accactggct gctcgacgga atgctgccgg
     4561 tggtcggttc cacctgcagc gtgaaggtga gctgggccac gccgggcccg aggtacggct
     4621 cggacaccga cagcgcacgg atgttgacgg ccggatcgac cggtgtgttc tggccggagt
     4681 cgttgtcgga gccgtcgctg ttgacgtcgc tcaccgaacg gaggcccgga acgatgcagg
     4741 cgttggccac ggggcccgag ttcggcgcca cggtctggca gtacgggccc tcgcccatcg
     4801 aattgacggc cgtcacgcgg taacggttct ggttggccgg gttcaccacg tggtcgaagg
     4861 ccgtctcgtt gacggtgccc accaccgaga acggcccggc atcgccggtg ctgctgtgat
     4921 acacgcggta ttcggtgatc gtggcaccgc cgttgtcagg cgcggtccag gccaggtgca
     4981 cgaccggtcc cacgatcccc gcggtcaggc cggccgcctg cggcacgccg ggttccaccg
     5041 ggtcgtacgc ggcgaagagg cgcttgcccc ccgactggcg cgtgatgacg gccttggccg
     5101 agaacgaatt gccgccgccg gtcacgcagc cgccgatgca gccgtcgtcc caacccacca
     5161 ggatgcgccc ttccttgtcc accgtgatgt cgaagaagtc gagcatgttg cggcagtcgc
     5221 cgtcgtcgca cacgccgccg cgctggatcg ggtcgcccgg cgtggcgttc acggtggtcc
     5281 aggtgttgcc gccgtcatag gtggtggcga tgtagaggta ccagacgccg gggaagtccg
     5341 gctggtcgta ggcgccggcc gtggtggtgc cgtaaaacgc gaaggcggcc cggtcgccgt
     5401 cacccgccac cacctccggg aagacgcagt tcacgatgcc gagcgaggtg cccacgtcgc
     5461 gcagcgggcc ccaggtcagt cccttgtcgg tcgatttcgc gatgcgggga tgtccgtccg
     5521 acgactgcca tcccaggtaa atgttgccgt tggtggccac gccgaccgac gggtcgtcat
     5581 cgatcatgcc gaccgagccg gggatcgggc gcacgtccca ggtgacaccg ttgtcttccg
     5641 acaccaccac cgcggcctgt ccgccgtcgt ggaacggcag tgtgccgccg caccccttgt
     5701 tgggaatgta caccgtgccg tccggcccca ccttgatgtg gccgtgcagg ccggcgcagt
     5761 cggcgaccgt gtacatcggg acggagggga ggaacgtcac gccgccgtcg aggctcagcg
     5821 agcagacggc gtcgccgacg cactgcgagg cgtagtacac cgcgtgcggg tacccgatgc
     5881 cggtgagcgg cgcgtgaaac gggccgccgc cgatcgtctg gtgatcgacg caggatggga
     5941 cgccggatcc ctgggtgggc agaaacgtat cgccatcatc gtcggtgaag tcggtcgtgc
     6001 tgccgagcgg cgtgagccct tcttcctggc tcacgaaggt gcgtccggtg acacggtccg
     6061 tgaagaggat ggggtcgccg aagacgcgcg gcgtgctggc ggtcaggagg ctcttctcct
     6121 cccacgtggc gatggccggc gactggcagt cgttgaacgt gatgcggagc atgtatggcg
     6181 agaagccgcc gaagtaggtg gtcgtgccgc cgttgggaat ggcgccatgg ctgttgctga
     6241 acgactgctc ggtcgtccag ttgctgccga tcgacggctc gccggaatca tcgccggaac
     6301 ctggcggtga atcgtaattc tggaatcgcg cggcgccggg caccgtcgcg tcgaggaccg
     6361 gcgtttccgc gtagccggaa gcaacgaatc ccggcgtggc gaggcacatc agcgccaccc
     6421 ccgcgatcca cgacaggcca ccgaaaatca cgggcacccg cacgcgcggt tgggttgcgg
     6481 tcatcacgta tctctgccag tcccaggaca tgatcgatcc tccttcggct ccaggccgtg
     6541 ccgtgcccgg cacgcgtcac tcgcatgcgg gcaacgggag gagaatgggg aggaagtatt
     6601 gattccgagt tgagtggagg atttgcgctg ccctttaagt aattgatgtg caagcaaata
     6661 cactattttc tgatgaaatt ctgctgattt cggccgccct caaccgtggc ggagtaacgg
     6721 gttgtgagga ctccgaacgc tggctaaact cccgcgttct tgaacccaag gagccccatg
     6781 tccctgagcg ccggccgcac gctctcccac taccgcctcg tcgagccgat cggcgagggg
     6841 gggatgggcg tggtgtggaa agcccacgac acctccctcg gccgcgatgt ggccgtcaag
     6901 atc
//
LOCUS       DASVOD010000024         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_105822, whole genome shotgun sequence.
ACCESSION   DASVOD010000024 DASVOD010000000
VERSION     DASVOD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105822"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF720_00450"
     CDS             complement(<1..833)
                     /locus_tag="VF720_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877845.1"
                     /translation="MRRLLPVATACLALGTLPLTGLPGTAAAAQDEEEHTTIGGYGEV
                     HYTNLSGPNTPGEVNVARFVVFLAHSFNERVSFRSELEVEDAKVEGGTPGGEVSVEQV
                     YLDYMLSPAATIRAGLVLPPIGIINETHEPPTFNGVDRPQFENDVIPTTWRDIGVGLV
                     GSIPGSSGLNYRVYLLNGLVASGFSAEEGIREGRQEGRDASFANPSLTGRLEWARPGF
                     RVGGSFWYGGSAAQDPALGTGTFDNAVALVSADARYDVGPLMFRAVVANVSIADAERI
                     NA"
     gene            860..>1523
                     /locus_tag="VF720_00455"
     CDS             860..>1523
                     /locus_tag="VF720_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013555859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877846.1"
                     /translation="MVINLKPYYFIPHCHWCQLPVPHIIWITPPLSPRTVRRLERGSP
                     RAYLPLRPVALPEASERVYDAWLDDLDGRLSAPGADWHRITRDVLFGLWYPGLSDYDA
                     LVEDPATPPVTRASLLALDPRNVTLEPEYYADVDAERYARVKPLHWLWQSFDRTPLGG
                     GNVQVGVRFRRILAPHLFARVGRNFKCFHFVEFSYGYNLEVGDDCVIHRYVLLDDRGG
                     ITL"
BASE COUNT          219 a          540 c          503 g          261 t
ORIGIN      
        1 gcgttgatgc gctcggcgtc cgcgatgctc acgttcgcca ccacggctcg gaacataagg
       61 ggcccgacgt cgtaccgggc gtcggcggag accagggcca ccgcgttgtc gaacgtgccg
      121 gtgcccagcg ccgggtcctg cgccgcgctt ccaccgtacc agaacgagcc gccgactcgg
      181 aagccgggcc gggcccactc gagccggccg gtgagcgacg ggttggcgaa gctggcgtcc
      241 cgcccctcct gccggccctc acggatgcct tcctcggccg agaatccgct ggccaccagc
      301 ccgttcagga ggtaaacgcg gtagttgagg cccgagctgc cggggatgct tccgacgagg
      361 ccgacgccga tgtcccgcca cgtggtcggg atgacatcgt tctcgaactg ggggcggtcg
      421 acgccgttga aggtgggcgg ctcgtgggtc tcgttgatga taccgatggg cgggagcacc
      481 agaccggcgc ggatggtagc ggccggcgac agcatgtagt cgagatagac ctgctccacc
      541 gagacctcgc caccgggcgt gccgccctcg accttggcgt cctcgacctc cagctcggac
      601 cggaaggaca cccgctcgtt gaagctgtgg gccaggaaca cgacgaaccg ggcgacgttg
      661 acctcacccg gcgtgttggg tccactgagg ttggtgtagt gcacctcgcc gtagcccccg
      721 atcgtggtgt gctcctcttc gtcctgggcc gcggcggccg taccaggcag cccggtgagg
      781 gggagtgtgc ccagggcgag gcaggcggtg gcgaccggga ggaggcggcg catggggcct
      841 ttcgtgactc tagttgaaaa tggttatcaa ccttaagccc tactacttta ttccccattg
      901 ccactggtgt caactgcccg tcccacacat catttggatc acgcccccgc tttccccccg
      961 aacggtccgt cgtctcgagc gaggctcccc gagggcctat ctgccgctcc gcccggtcgc
     1021 cctccccgag gcgtccgagc gtgtctacga cgcctggctg gacgacctgg acggccgcct
     1081 gtcggctccg ggcgccgact ggcatcggat cacccgcgac gtcttgttcg ggctgtggta
     1141 ccccggcttg agcgattacg acgccctggt ggaggatccg gccacccccc cggtcacgcg
     1201 cgcatcgctc ctggccctcg acccccgcaa cgtcaccctc gagcccgagt actacgccga
     1261 cgtcgacgcc gagcggtacg cccgggtcaa gccgctccac tggctgtggc agagcttcga
     1321 ccgtacgccg ctcggcggcg gcaacgtcca ggtcggcgtg cgcttccgcc ggatcctcgc
     1381 cccccacctc ttcgcgcggg tcggccgcaa cttcaagtgc tttcatttcg tggagttttc
     1441 ctacggctac aacctggagg tgggtgacga ctgtgtcatc caccgctacg tgctgctcga
     1501 cgaccgcggc gggatcacgc tcg
//
LOCUS       DASVOD010000025         3232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_888240, whole genome shotgun sequence.
ACCESSION   DASVOD010000025 DASVOD010000000
VERSION     DASVOD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888240"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="VF720_00460"
     CDS             <1..1738
                     /locus_tag="VF720_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7877847.1"
                     /translation="PARAQSPTSGENHPVAPSDPGRDLLLFEADNAIRGGDYRAAIDA
                     LEKILRKNPNDEEALEKLAQTWSRWAESGEASPDSLRTLTLDVYRRLLEENPRQPRAL
                     LNLRRLSPDEWRQYMARAATAESGGNEREAETCLRIAFELSDTSAVLRERLGTLTRRL
                     ADKAPEPERTFYMGRLDLAAGRPFEAAARFRQCLDADSTIAEFRKYYGIALYEQERFA
                     DALTWLQPLAESQPEDPDVRFYIGSLLFRLQHWQPALEQLLPVAEQHLYRGEASRMVG
                     LALARSGVHDEVALKYLEQAATDGSTDTDLPCQIGEHYVRLDRWDEARTSFEKCRQGS
                     PDHPAAHLGLGLVADEQGKWKESIEHFEKFLVTNPKSPAVLIRSGVSWIRIDQPDTAA
                     ARFRAAIRADSVFAAMPADSINDAQAFEVAFLVLLAGREYRDGIVVGEYLLKREPENP
                     TYLNNLAMALADAGQNLDRGLDLAQRANAASPDNPGFQDTLGWVQVRMKRYGDASKTF
                     DRSLELAEKVGMTDIAEILYHRAVMHASNNERMKAIDDLNKTLAGDPNPQIRRSAEAL
                     LKRLLEEQKKLD"
     gene            1755..3131
                     /locus_tag="VF720_00465"
     CDS             1755..3131
                     /locus_tag="VF720_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7877848.1"
                     /translation="MTFDRARLAQIDAAIDASLESNLADLARLVAIPSVAAQNRGIEP
                     CAKLVRDMLVERGYQAEICPTDGNPVVCGEGAGASDRTLLFYNHYDVQPAEPLELWDS
                     EPFVMVRRDGKVFGRGISDDKGHFIARLHALDAVRQARGGTLPCRVKFVVEGEEEVGS
                     VHMPAFVEKNRERLAADACVWEFGGEDDEGRPEQYLGMRGICYVELTVRTANIDAHSG
                     MAGSIFHNAAWRLTWALATLKGKDERILIPGHYDSVKAATPRDLEMLAAMPDTAPEMT
                     ERYELKGFLNKLTGGVELRRAESFEPTCTICGLESGYQGAGSKTVLPAFARAKVDFRL
                     VPDQSPEEVLANLRKHLDAQGFTDVEITYLGGGKPARTDPDHPFITMSNEAARDAYGK
                     PPVVAPMVGGSGPNWPFTHVLNVPITMCGIGYPGGQAHAPNEHFVLEHYMKGVKHTAR
                     IVEGFAAL"
BASE COUNT          573 a         1175 c         1026 g          458 t
ORIGIN      
        1 cccggcccgg gcccagtctc cgacgtcggg cgagaaccac ccggtggccc cctccgatcc
       61 cgggcgcgac ctcctcctct tcgaagccga caacgccatc cgtggcggcg actaccgcgc
      121 ggccatcgac gcgctggaaa agatcctccg gaagaacccg aacgacgagg aggcgctgga
      181 gaaactggcg cagacctggt cgcgctgggc cgagtccggg gaggccagcc cggattcgct
      241 gcgcacgctc acgctcgatg tgtatcggcg cctgctcgag gagaatccgc gacagccccg
      301 cgcgctcctg aatctccgga ggctgtcgcc agacgagtgg cggcaataca tggcccgggc
      361 cgccacggca gaatcgggcg gcaacgagcg ggaggccgaa acctgccttc gcatcgcctt
      421 cgaactgtcg gacacgagcg cggtgctccg cgagcggctc ggcacgctca cgaggcgcct
      481 ggccgacaag gcaccggagc cggaacgcac cttctacatg ggtcggctcg accttgccgc
      541 cgggcgcccc ttcgaggcgg ccgcccgatt ccgccagtgt ctcgatgccg attccacgat
      601 cgcggagttc cgcaagtact acggcatcgc cctgtacgag caggaacgct ttgccgacgc
      661 gctcacctgg ctccagccgc tggccgaatc gcagccggaa gatccggatg tccgcttcta
      721 catcggctcg ctcctcttcc ggctgcagca ctggcagccg gcgctcgagc agctcctccc
      781 ggtggccgag cagcatctct accgcggcga ggcctcgcgg atggtgggcc tcgcgctcgc
      841 gcgttccggc gtgcacgacg aggtggcgct gaaatacctc gagcaggcgg ccaccgatgg
      901 ctcgaccgac accgacctcc cctgccagat cggcgagcac tacgtgcgcc tcgaccgctg
      961 ggacgaggcc cgcacctcct tcgagaagtg tcgccaggga agcccggatc acccggccgc
     1021 ccacctgggg ctcggcctcg tcgccgatga gcaggggaag tggaaggaat cgatcgaaca
     1081 cttcgaaaag ttcctcgtca cgaatccgaa atcgccggcc gtgctcatcc gctcgggcgt
     1141 cagctggatc cgcatcgacc agcccgacac ggccgcggcc cgcttccgtg ccgccatccg
     1201 ggccgacagc gtcttcgccg ccatgccggc cgattcgatc aacgatgccc aggcgttcga
     1261 agtggcgttc ctcgtgctgc tggccgggcg cgagtatcgc gatggcatcg tggtcggcga
     1321 gtatctcctg aagcgggagc cggagaaccc gacctacctg aacaacctgg cgatggccct
     1381 ggccgacgcg ggacagaacc tcgatcgcgg cctcgacctg gcccagcgcg ccaacgcggc
     1441 atcgcccgac aaccccggct tccaggacac gctggggtgg gtgcaggtgc gcatgaagcg
     1501 gtatggcgac gccagcaaga cgttcgatcg ctcgctggag ctggccgaga aggtcgggat
     1561 gaccgacatc gcggaaatcc tctaccaccg cgccgtgatg cacgcctcga acaacgaacg
     1621 gatgaaggcc atcgacgacc tcaacaagac gctggccggc gatcccaatc cccagatccg
     1681 acgctccgcc gaggcgctcc tgaagcgcct cctcgaggaa cagaagaaac tcgactgacc
     1741 ggaaggaact cgccgtgacc ttcgaccgcg cccgccttgc ccagatcgac gccgccatcg
     1801 acgcctccct cgaatcgaac ctggccgacc tggcccgcct cgtggccatc ccgagtgtgg
     1861 cggcccagaa ccggggcatc gagccgtgcg cgaaactcgt gcgcgacatg cttgtggagc
     1921 gcggctacca ggccgagatc tgcccgacag acggcaaccc ggtggtgtgc ggcgaaggcg
     1981 cgggtgccag cgaccgcacg ctcctcttct acaaccacta cgacgtccag cctgccgagc
     2041 cgctcgagct ctgggattcc gaaccgttcg tcatggtgcg ccgcgacggc aaggtgttcg
     2101 gccgcggcat ctcggacgac aaggggcact tcatcgcccg gcttcatgcg ctcgacgcgg
     2161 tacgccaggc ccgcggcggc accctcccct gccgggtgaa gttcgtggtg gagggcgagg
     2221 aggaggtcgg cagcgtgcac atgccggctt tcgtcgagaa gaaccgggag cgactggccg
     2281 ccgatgcgtg cgtgtgggag ttcggtggcg aggacgacga ggggcgcccc gagcagtacc
     2341 tcggcatgcg cggcatctgc tacgtggagt tgacggtgcg taccgccaac atcgacgccc
     2401 actccggcat ggccggctcg atcttccaca acgccgcctg gcgcctcacg tgggcgctgg
     2461 ccacgctcaa gggcaaggac gaacgcatcc tgatccccgg tcactacgac tccgtgaagg
     2521 ccgccacccc gcgcgacctc gaaatgctgg cggccatgcc cgacaccgcc cccgaaatga
     2581 ccgagcgcta cgagctcaag ggattcctga acaagctgac cggcggcgtg gaactgcgcc
     2641 gagccgagag cttcgagccc acctgcacca tctgcgggct cgaatccggc taccagggcg
     2701 ccggctcgaa gaccgtgctg ccggccttcg ccagggccaa ggtcgatttc cgcctggtgc
     2761 ccgaccagtc gcccgaggaa gtgctggcca atctgcgcaa gcacctcgat gcgcagggct
     2821 tcaccgacgt ggaaatcacc tacctcggag gcggcaagcc ggctcgcacc gaccccgacc
     2881 acccctttat caccatgtcc aacgaggccg cccgcgacgc ctacggcaag ccccccgtgg
     2941 tggcccccat ggtcggcggt tccgggccca actggccctt cacgcacgtg ctgaatgtcc
     3001 ccatcaccat gtgcggcatc ggctaccccg gcggccaggc ccacgcgccg aacgagcact
     3061 tcgtactgga gcactatatg aagggggtca agcacaccgc gaggatcgtg gaaggcttcg
     3121 ccgcgctcta gggcaacccg gcccgaaccc gcttgacgcc cccccaaggg cggtgtctcc
     3181 tttcccccaa tgagcaaacc cgacgaagcc aaacgccgct ccacgttcat ca
//
LOCUS       DASVOD010000026         9029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_992857, whole genome shotgun sequence.
ACCESSION   DASVOD010000026 DASVOD010000000
VERSION     DASVOD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992857"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VF720_00470"
     CDS             complement(<1..472)
                     /locus_tag="VF720_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877849.1"
                     /translation="MSTPEVDRLVEAVAQEVTRRLAAEYQVQVARTPKDLPCTGCGHC
                     AQKCSDNVKGVLAAGADRIASSPGTINVESAIAGFIDHTILKPDATPKEVETVCDEAL
                     KYGFAAVCVNPTWVRLVARKLRGSNVTVCSVVGFPLGASPTRAKVSETRGAIRDG"
     gene            complement(469..1077)
                     /gene="rpiB"
                     /locus_tag="VF720_00475"
     CDS             complement(469..1077)
                     /gene="rpiB"
                     /locus_tag="VF720_00475"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEX7877850.1"
                     /translation="MISDRELREVVRRVVEASLPGGNDGGTTLPAEPATPSATPPEPA
                     SGGARRLAIGADHGGYELKERLKPFLLDLGWAIEDVGTWSEQAVDYPAIALKVADLVA
                     AGTTAVGIVIDGAGIGSCMAANKVPGVRAALCHDTTTARNSREHNDANVLTLGGKLIG
                     VVQAQEIVKTWLATECREERHKKRVAMITAIESRAGGAPAPR"
     gene            complement(1123..2027)
                     /locus_tag="VF720_00480"
                     /pseudo
     CDS             complement(1123..2027)
                     /locus_tag="VF720_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
     gene            complement(2086..2538)
                     /locus_tag="VF720_00485"
     CDS             complement(2086..2538)
                     /locus_tag="VF720_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877851.1"
                     /translation="MSHHPESGDRANESAATRFDGGSFPTGLVLTMVVIIGLAIWQFG
                     SLAPKDQDFPTDDVDTLIRGNEVLVFNPTHGLLDIYQVTIQRLSGSYVYFGRNLKPRS
                     IKHVKLTDLRTESGVRFDPKEEGECRLMLEYWRGTEKEGPFFRYCRGF"
     gene            complement(2586..3371)
                     /locus_tag="VF720_00490"
     CDS             complement(2586..3371)
                     /locus_tag="VF720_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEX7877852.1"
                     /translation="MFQAFTILLREGVESFIVVAIIVTYLMKTGRGSLLPAAWSGIAT
                     ALVASIAGTWLLSRFGESSLQEGVVCLIAAALTGTMVVWMWKTGKNLRRDIENQLEEA
                     SQKHDKSPSFWAAVGVFTAAFLLIAREGAEAGLLIVSLVRTTNQSSALLGALLGLALS
                     IGLGMLWIQNSRRMNLKAFFQVTGLFLLVFVAQLTLSGVHELSEAGVIPYAEQIHLAT
                     EPWVEQGVVARWITLIMVLIPGGWLLYSAFKRPPAVPASSATK"
     gene            complement(3408..3938)
                     /locus_tag="VF720_00495"
     CDS             complement(3408..3938)
                     /locus_tag="VF720_00495"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX7877853.1"
                     /translation="MGIESWKSFEKNEVTHSMAHYLMAIRDLLTSRGYARATDVAEYL
                     DITRASASVAIGGLRGKGLVEEDDKHFLKLTEHGQELVRTILTNRRLLICFLRDVLMV
                     DPVVAEEEACKTEHLLSSESGTRLFRLLRYLLADSNRTERVREVVASLNDECPGLSRC
                     EICSEFHECILGQANH"
     gene            complement(4028..6643)
                     /locus_tag="VF720_00500"
     CDS             complement(4028..6643)
                     /locus_tag="VF720_00500"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7877854.1"
                     /translation="MRRGFWLLLVLLWTMAAPATAPSPASAEIVRLPTDVVPTYQEVS
                     LKLDPADPAFSGSVRIELAVRAPVTSFRLHAPTARIGTITLHDSAGLVAATVDSADAQ
                     EQVTFTLSRPLEAGPAVLELTFTNVFDVRAIGLYRAKAGNDWYAMSQMEPDDCRLAFP
                     CFDEPAFKIPWRLTLEIPDTLSAVTNVPEASSAPASSGWKKIVFRKTPPMPSYLVAVC
                     VGAWEYVEVPGVSVPTRIVTARGMSGHVSLARELTPPICQALEEWFGSPMPYEKLDLI
                     SAPEFWPGAMENPGAILFADRNLIQDAATAGRAARRQLTSLIAHEVAHLWFGDMVTMA
                     WWDDLWLNEAFAAWMGDRITDQLYPEFRSPITELVQTQRFLAMDARGVAPAIRQPVES
                     THDLLGNVGLVYSKGKRILSMFQSWVGPEAFQSGVRQYLATNAWRNAEAKDLWNALSV
                     AGGVDVASALPTFLDQPGCPLIGVRGMPGGRIELTQRPLSAGPGPASDRRWSVPVGLS
                     AGLPDSITRVTALVPGPESAYALGVPRAPAWIYPNRGGTGYYRWMVSPPALVALADSA
                     SRWLEPIERAEVSGNLTGLMEAGLINSGVWLNLQGKLAGDVEPMVVAAVSDNLAGNLS
                     LLVDKADRPALAAWIRTTFRPALDRIGMQPKPDEPPGATFLRPRLINLLGRLGDDGDV
                     IKFSRRQLDDWLANPEAVDPALRQDAFRVAVYRGDAALWEKLHAAFQVTRRPPDRRLL
                     LLTLGAFGDPVLARKSLDSLHDPGVRPTEFFPLLGEAASDEAGKDVVFAWVRDDYAAL
                     ASKVGPGLMPFLVRQLGGVSKEREAAVAAWFNDPAHAAPGTATELSRVVQDGEALRLL
                     RNRESALLGQYLRRL"
     gene            complement(6667..6744)
                     /locus_tag="VF720_00505"
     tRNA            complement(6667..6744)
                     /locus_tag="VF720_00505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6707..6709),aa:Arg,seq:cct)
     gene            complement(6897..7454)
                     /locus_tag="VF720_00510"
     CDS             complement(6897..7454)
                     /locus_tag="VF720_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877855.1"
                     /translation="MRFANTARAVLVGSLVTLSAGVASAAHLTIIDTASDVTVQPDGN
                     FEFGFNSVQNNQHDWSFSGSWIADSGSTGEGVIYFVDPNTNELEDIYTLSWTYDGFFA
                     ATLSGRFESDNGGVIASGRTIPAGFPTVDVTNGNVDVLGLFRDPTTGAPVSVPSNITM
                     LVNFDYAPVATEASSWSAIKGKLAR"
     gene            complement(7700..8602)
                     /locus_tag="VF720_00515"
     CDS             complement(7700..8602)
                     /locus_tag="VF720_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7877856.1"
                     /translation="MSDLTIRAAMTTDVPAIAAFIVRMNAVSDQRCLHCGDSIADVMK
                     SLSTLGLPPERSFYLAFMGERIVGLIGADLATDIGRGWLWGPYADVAEPATVASALAA
                     RFLDDLPDGIAVVDSFLDTNNRFTGLLADAHGFEAPRIVHFYRAPRPTGATPAGAPMA
                     DDLKTDVADTHLPLLNALHEASFPGTWRSVDQMRASDGNSAVLYVAEEGGVFQGYVYS
                     EAAPEEETGHVHYLAVMPEWRGRGVGRRLLDAALAWCFVARDLPSASLTVFDTLTDAR
                     SLYESAGFRLLESGVTRRWTRPGA"
     gene            complement(8656..>9029)
                     /locus_tag="VF720_00520"
     CDS             complement(8656..>9029)
                     /locus_tag="VF720_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877857.1"
                     /translation="NLHVVYTSRVQLPGGGDELFSPVLESLPGAVNDDVRWNEVEVIF
                     TPGSIPRQYTSSTEVISAMAAGDVVLTPTGARYAMNLISISPDDPPGERMVRGDLLKP
                     IAVPDEDVNVTWGYVKGRIRR"
BASE COUNT         1574 a         2987 c         3021 g         1447 t
ORIGIN      
        1 cgccgtcacg gatggcgccg cgcgtttccg acaccttggc ccgggtgggc gaggcgccga
       61 gcggaaagcc caccaccgaa cagaccgtca cgttcgagcc gcggagcttc ctggccacca
      121 ggcgcaccca ggtggggttc acgcagaccg cggcaaagcc atatttcagt gcctcgtcgc
      181 acaccgtttc gacttctttc ggcgtggcgt cgggcttcag gatcgtgtgg tcgatgaagc
      241 cggcgatggc cgactccacg ttgatcgtgc ccggcgaact ggcgatgcga tcggcccccg
      301 cggcgaggac ccccttcacg ttgtccgagc acttctgcgc gcagtggccg cagccggtac
      361 aggggaggtc tttcggcgtg cgggccacct gcacctggta ttcggcggcc aggcggcgcg
      421 tgacctcctg cgccacggcc tcgaccaggc ggtcgacctc gggggtgctc atcggggcgc
      481 cggcgcgcct cccgcgcgcg attcgatggc ggtgatcatg gctacccgct tcttgtggcg
      541 ctcctcgcgg cactcggtgg cgagccaggt cttcacgatc tcctgggcct gcacgacgcc
      601 gatcagcttt ccccccagcg tgaggacgtt ggcgtcgttg tgctcgcggc tgttccttgc
      661 ggtggtcgtg tcgtggcaga gcgcggcgcg cacgccgggc accttgttgg cggccatgca
      721 ggagccgatg ccggcgccgt cgatcacgat ccccacggcc gtcgtgccgg cggcaacgag
      781 gtcggcaacc ttgagggcga tggcgggata gtccaccgcc tgttcgctcc aggtccccac
      841 gtcttcgatg gcccagccga gatcgagcag gaagggcttc aggcgctcct tgagctcgta
      901 gccgccatga tcggcgccga tggccaggcg gcgggccccg ccggaggccg gctccggagg
      961 ggtggcggag ggtgtggcgg gttcggccgg gagcgtggtt cccccgtcat tcccacccgg
     1021 caggctggct tccaccaccc gccggaccac ctcgcgcagt tcacgatcgg aaatcatgca
     1081 acctccccgg acgcggcaac ttaacacacc ccgtcacgga cttcatcccc cggcccgggc
     1141 ggccgatcgc gtccttcccc gggccggctc gaggtgcacc agcacctcgc gaatttcggg
     1201 gtgggtttcg agcaggcgat ccttcacggc gtgccccacg cggtggcccg cctccacggt
     1261 catggcggga tcgacctcca gatggagatc gacgaggtac tcgagcccgg ttttccggac
     1321 gaggagtttt tccacggcgg ccacgtccgg cacgtccagc gcaatccggc gaagccggtc
     1381 cacgtactcc ggctcggcct gcgcatccat cagctcatgg atgctgccga gcaggaggcg
     1441 cagtcctccc cacagaatta ccgccaccac gcagagcgcc gcgatttcat ccgcgctgcg
     1501 ccagtcgggc ccgccgaaac gcaccagtga cagcccggcc agcaccgcga gtgccgccag
     1561 ggcatccgcg cgatgatccc aggcgttggc cagcgtggcc tgcgaacccg acagacggcc
     1621 gatgcgtccc tggtagcggt aaagagcctc cttgaggacg atggtcaggc cggccaccca
     1681 gagcgtccag gccggcggtg cggcgtgatc gaggggaatc cgccggaatg cctccacggc
     1741 gatggcccag cgctggccag gatcagcacc gacacgatgg ccccggccac ggcctcgatt
     1801 cgcgtgtggc cgtaggggtg gccggcatcc gggggccgct gggcgaaacg gagcgaggag
     1861 aggacgacga tggaggcgag gacatcgccc agcgaattga tggcatcggc gatgagcgcg
     1921 aaggagccgg agaagagtcc ggccgcgagt ttgccggcgg ccagcacgag attggcgagg
     1981 agcccggtca gcgcgcccca ttccgcgcgg gcgtacacgg accgcatcgc cccgacaggg
     2041 cgtccgacga cgggcccgga ggcggcgctc acacgattac cggcgtcaga acccgcggca
     2101 gtaacggaag aacggacctt ccttctcggt gccgcgccag tactcgagca tcagccggca
     2161 ctccccttct tccttcggat cgaagcgcac gcccgactcg gtgcgaaggt cggtgagctt
     2221 cacgtgcttg atgctgcgcg gcttcaggtt gcgcccgaag tagacgtacg agccggagag
     2281 gcgctggatc gtcacctggt agatgtcgag caacccgtgg gtgggattga acaccaggac
     2341 ctcgttgccc cggatcagcg tgtcgacgtc gtcggtggga aaatcctggt ccttgggcgc
     2401 caggctcccg aactgccaga ttgccaggcc gatgatgacg accatggtga ggaccagccc
     2461 ggtcggaaag gagccgccgt cgaaccgcgt ggcggccgat tcgttggcgc ggtcgcccga
     2521 ttcggggtga tgggacatgg gcgcatctta gcaggatgcc gggagcgttg cccccgttcg
     2581 catcgctact tcgtggcgct ggaggccgga acggcgggcg gccgcttgaa cgccgagtag
     2641 aggagccagc ctccggggat cagcaccatg atcagcgtga tccagcgcgc cacgaccccc
     2701 tgctcgaccc acggctcggt ggccaggtgg atctgctcgg cgtacggaat cacgcccgcc
     2761 tcggagagct cgtgcacgcc cgagagcgtg agctgggcca cgaagaccag caggaagagt
     2821 ccggtcacct ggaaaaacgc cttcaggttc atgcgccggc tgttctggat ccagagcatg
     2881 ccgaggccga tggaaagcgc caggccgagg agcgcgccga ggagcgccga gctctggttg
     2941 gtggtgcgga cgaggctcac gatcagcagg ccggcttccg ccccttcgcg ggcgatcagg
     3001 agaaaggccg cggtgaacac gcccaccgcc gcccagaagc tgggggactt gtcgtgcttc
     3061 tggctggcct cctcgagctg gttttcgatg tcgcggcgga ggttcttgcc ggtcttccac
     3121 atccacacca ccatcgtgcc ggtcagtgcc gccgcgatga ggcagaccac gccttcctgc
     3181 agcgagctct caccgaatcg cgacaggagc caggttccgg caatcgaggc gaccagcgcc
     3241 gtggcgatgc cgctccaggc ggccggcaga agcgagcccc gccccgtctt catgagatac
     3301 gtgacgatga tggccacgac gatgaacgat tcgacgcctt cgcggagcag gatggtgaac
     3361 gcctggaaca ctcaagtcct cgcagtcgat gggtggtttg cggcggccta gtggtttgcc
     3421 tggccgagga tgcattcgtg gaattcggag cagatttcgc agcgcgacag tcccgggcac
     3481 tcgtcgttga gcgatgccac gacctcgcgc acccgctcgg tccgattgga atcggccagg
     3541 agatagcgaa ggaggcggaa gagccgcgtg cccgattcgg aactcaggag atgctcggtc
     3601 ttgcaggctt cttcctcggc cacgacggga tcgaccatca gcacatcacg caggaagcag
     3661 atgagaaggc gccggttcgt gaggatggtg cggacgagtt cctggccgtg ctcggtcaac
     3721 ttgaggaagt gcttgtcatc ctcctcgacc agtcccttgc cccgcaggcc gccgatggcc
     3781 accgaggccg aggcgcgcgt gatgtcgagg tactcggcca cgtccgtggc cctggcatag
     3841 ccgcggctgg tcaggagatc ccggatggcc atcaggtaat gggccatgct gtgggtcact
     3901 tcgttcttct cgaacgattt ccagctctcg attcccatcg gttcttcgat tcccccgcca
     3961 gtgtggcttg acgcttgtta aagatgccta acggatgttc ggtccgtcaa gcagtaatcg
     4021 taacttgtta caggcgccgt agatattgac cgagcagggc cgattcccgg ttccggagca
     4081 gccggagagc ctcaccgtcc tgaacgaccc gcgagagctc cgtggccgtt ccgggcgccg
     4141 cgtgggcggg atcgttgaac caggccgcca ccgccgcctc gcgctctttc gagacgccgc
     4201 caagctggcg caccaggaac ggcatcaggc cgggccccac cttggaggcc agcgcggcgt
     4261 agtcgtcccg gacccaggcg aagaccacgt ccttccccgc ctcgtcactg gccgcctcgc
     4321 cgagcagtgg aaagaactcc gtcggccgga caccggggtc gtggagcgag tcgaggctct
     4381 tccgcgccag gaccggatcg ccgaaggcgc cgagcgtgag caggaggagc cgtcgatcgg
     4441 gcggacgccg cgtgacctgg aacgcggcgt gcaatttctc ccagagcgcg gcgtcaccgc
     4501 gatacacggc cacgcggaac gcgtcctgcc gtaatgccgg atcgaccgcc tccggattgg
     4561 ccagccagtc atcgagctgg cgacggctga acttgatgac gtcgccgtcg tcgccgaggc
     4621 ggcccaggag gttgatgagg cgcggccgca ggaacgtggc ccccggcggt tcatcgggct
     4681 tgggctgcat gccgatccgg tcgagcgccg ggcgaaacgt ggtgcggatc caggccgcga
     4741 gggccggacg atcggccttg tcgacgagca gcgagagatt gcccgccagg ttgtcggaaa
     4801 ccgccgccac gaccatcggc tcgacatcgc ccgccagttt cccctgcaga ttgagccaca
     4861 cgccgctgtt gatgagcccg gcctccatga ggccggtcag gtttcccgac acttcggcgc
     4921 gctcgatcgg ctcgagccag cgggacgcgc tgtcggccag cgccaccagt gccggtggcg
     4981 acaccatcca gcggtaatag cccgttcccc cgcgattggg atagatccac gccggggcgc
     5041 gcggcacacc cagcgcgtag gccgactcgg gccccggcac cagcgccgtg acgcgcgtga
     5101 tcgagtccgg aagaccggcc gacagcccga ccggcaccga ccagcgtcga tcggacgccg
     5161 gacccgggcc cgcgctcaag gggcgttgcg tcagctcgat ccgcccaccc ggcatgccgc
     5221 gcacgccgat caaggggcat ccgggctggt cgagaaacgt cggcaatgcc gaggccacgt
     5281 ccacgccgcc ggccaccgac agggcattcc agaggtcctt tgcctcggcg ttgcgccagg
     5341 cattcgtcgc gaggtactgg cggacaccgg actggaaggc ctcggggccc acccacgact
     5401 ggaacatcga caggatccgc ttccccttcg aatagacgag ccccacgttt ccgagcaggt
     5461 cgtgcgtcga ctcgaccggc tggcggatgg cgggcgccac gccacgggca tccatggcca
     5521 ggaagcgctg ggtctgcacc agttcggtga tcggcgaccg gaattccggg tagagctggt
     5581 cggtgattcg atcccccatc caggccgcaa acgcctcgtt cagccagagg tcgtcccacc
     5641 aggccatcgt gaccatgtcg ccgaaccaga ggtgcgccac ttcatgcgcg atcagcgacg
     5701 tgagctgccg ccgcgcggcc cgccccgccg tggccgcatc ctggatgaga ttgcggtcgg
     5761 cgaagaggat ggcgccgggg ttctccatcg cccccggcca gaactcgggc gccgagatga
     5821 ggtcgagctt ctcgtacggc atcggcgagc cgaaccactc ttcgagtgcc tggcagatcg
     5881 gcggcgtgag ctcgcgcgcc aggctcacgt gtcccgacat cccgcgtgcc gtcacgatgc
     5941 gggtgggcac cgacacgccc ggcacctcca cgtattccca cgcccccacg cagactgcca
     6001 ccaggtagga gggcatcgga ggcgtcttcc ggaagacgat cttcttccat cccgatgacg
     6061 ccggtgcgct cgacgcctcg ggcacgttcg tcaccgccga gagcgtgtcc gggatctcga
     6121 gcgtgagccg ccacgggatc ttgaacgccg gctcgtcgaa gcagggaaag gccagccggc
     6181 agtcatccgg ctccatctgc gacatcgcgt accagtcgtt gccggccttc gcgcgataga
     6241 ggccgatggc ccggacatcg aagacgttgg tgaacgtgag ctcgaggacc gccggtcccg
     6301 cctcgagcgg ccgggacagg gtaaaggtca cctgttcctg cgcgtcggcc gagtcgaccg
     6361 tggcggccac gaggcccgcc gaatcgtgca acgtgatcgt gccgatccgg gcagtcggcg
     6421 catgcagccg gaaactcgtc accggggcgc gaaccgcgag ctcgatcctc accgatccgg
     6481 aaaaggcggg atcggccgga tcgagtttca gggagacttc ctggtacgtg ggaacgacgt
     6541 ccgtggggag ccggacgatt tccgcggacg cgggactcgg cgcggtggcg ggagcggcca
     6601 tcgtccacaa caggaccagg agtagccaga aacctcgacg cacgcatccc cccatgaaag
     6661 gcgaaatggt gcccctgact gggatcgaac cagcgacacg cagtttagga aactgctgct
     6721 ctatccatac tgagctacag gggcacgagc gcgaaattgt agcgtgattg gcggttaccg
     6781 gcgagtccct tgtccgggcc cacgtttccg gatcgtgttc gaaatcgatt ggtcccgcaa
     6841 acaaacgggc ccgtggcaag ccacgggccc gttcgattgg aagcgttcga gtgaaatcag
     6901 cgagccagct tgcccttgat cgcggaccac gacgaggcct cggtcgccac cggggcgtag
     6961 tcgaagttga ccagcatcgt gatgttcgac ggcacgctga ccggcgcgcc ggtcgtcggg
     7021 tcgcggaaca ggccgaggac gtcgacattg ccgttcgtga catcaacggt cgggaaaccg
     7081 gcggggatcg tacgacctga agcgatgacg ccaccattgt ccgattcgaa ccggccactc
     7141 aaggtggcgg caaaaaaacc atcatacgtc cacgacagcg tgtagatgtc ctcgagctcg
     7201 ttcgtattcg gatcgacgaa gtagatgacg ccctcgccgg tcgagccgga gtcggcaatc
     7261 caggatccgc tgaacgacca gtcgtgctgg ttgttctgga cgctgttgaa tccgaactcg
     7321 aagttgccgt ccggctgcac cgtgacgtcg gacgcggtgt cgatgatcgt gaggtgggcg
     7381 gccgaggcca ctcctgccga cagggttacc aatgatccga ccagaacggc acgtgcggta
     7441 ttcgcaaagc gcatgatgtc caacctccta atgaggccac ttgctgttgt ctgaggcggt
     7501 tcctcaaacg gatgggcgat ggaagttatg acaacagcgg aacaggggtc aatcactttc
     7561 acctttcggg acaatcggaa cattgcgaaa gttgatcact ttcgctcgcc tcggggcgag
     7621 ttcgtcgaca agtcaggaaa aatttacgga cgcgtttcta cccttaccga gatcatgagg
     7681 cccgtcacgt cgggcgcggt cacgcacccg gtcgcgtcca gcgtcgcgtg acgccgctct
     7741 ccagcaggcg aaatccggcc gactcgtaga gtgaacgcgc gtcggtcaac gtgtcgaaga
     7801 ccgtgagcga ggccgagggc agatccctgg ccacgaagca ccatgccagt gcggcgtcga
     7861 gcaggcgccg ccccacgccc cgcccccgcc actcgggcat gaccgccagg tagtggacgt
     7921 gacccgtctc ctcttcgggt gcggcctcgg agtagacgta gccctggaag acgcccccct
     7981 cctcggcgac gtacaggacg gcgctgttgc cgtcggaggc tcgcatctgg tcaacgcttc
     8041 gccaggtgcc cgggaaggag gcttcgtgaa gcgcgttgag gagcggcagg tgagtgtccg
     8101 cgacatcggt cttcaagtcg tcggccatcg gagcgccggc aggagtcgct ccggtcggtc
     8161 gaggggcccg atagaagtga acgatgcggg gcgcctcgaa gccatgcgca tccgcgagga
     8221 ggcccgtgaa acggttgttc gtgtccagga aggaatcgac taccgcgatc ccgtcgggca
     8281 ggtcgtccag gaatcttgcg gcaagtgcgc tcgccaccgt cgccggctcc gcgacatcgg
     8341 cgtacggccc ccacaaccac ccccgaccaa tgtcggtggc gagatcggcg ccgatcaggc
     8401 cgacaatcct ctcccccatg aaggccaggt aaaaggatcg ctcgggcggg agcccgagcg
     8461 tcgacaggct tttcatgaca tcggcaatcg agtcgccgca atgaaggcac cgctggtcgg
     8521 acaccgcgtt catccggacg atgaacgcgg cgatggcagg aacatcggtc gtcatggctg
     8581 cccggatcgt caggtcactc atcggggcag tttaatccaa accgtgcgac ccactctcag
     8641 acgaggccgc cgcggtcaac gccggatcct tcccttcaca tacccccagg tgacattcac
     8701 gtcctcgtcg ggcactgcga tcggcttcag cagatcgcct cgcaccatgc gctctcccgg
     8761 cgggtcatcg gggctgatgc tgatcaggtt catcgcgtaa cgcgcgccgg ttggcgtgag
     8821 gacgacatcc ccggccgcca tcgcgctgat cacttcggtg gacgacgtgt actgacgggg
     8881 aatgcttccg ggagtgaaga tcacctcgac ttcgttccaa cgtacatcgt cattcaccgc
     8941 gccgggaagg ctctcgagga ccgggctgaa gagttcatcg cccccacccg gaagctgaac
     9001 ccgggatgta tagacgacat gcaagttcg
//
LOCUS       DASVOD010000027         3990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_314440, whole genome shotgun sequence.
ACCESSION   DASVOD010000027 DASVOD010000000
VERSION     DASVOD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314440"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..2920
                     /gene="uvrA"
                     /locus_tag="VF720_00525"
     CDS             89..2920
                     /gene="uvrA"
                     /locus_tag="VF720_00525"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX7877858.1"
                     /translation="MDRISIRGAREHNLKNISIDIPRDRLVVLTGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLGQMEKPDVDQIEGLSPAISIEQRTAGRNPRSTVATVT
                     EIYDYLRLLFARVGIQHCHNCGRRISRMTIQEMVDVALKQPEGARAQLLAPIVKGRKG
                     EYRAELEQLKKQGYLRARIDGELYELEEPPKLAKTKKHTIEIVVDRLKIDPKNQGRLA
                     DSLETALKLGQGQATLSVDGVAEEILFSEAASCLHCGLSFDELEPRMFSFNSPYGACP
                     KCDGLGTQMEIDIDLLIPDDSLSLRDGALAPMRTVEDGWYGQQITALAKNYKFSLDTP
                     FRKLTKKQQEVLLNGSGDEEITVQYKDKRYDVKFKKKFEGVIPNLMRRHRETSSNWIR
                     EWIEGYMTLRPCTACDGARLKPSSLAVSVQGDSIRDVTSRSVSRSLEWARALVLTERE
                     ATIATAIMKEVTSRLDFLSDVGLGYLTLDRTAGTLSGGEAQRIRLATQIGSKLMGVLY
                     ILDEPSIGLHHRDNRKLLNTLHHLRDLGNTVIVVEHDQETMEEADFLVDLGPGAGKHG
                     GEIVVAGPPPVVRDHPDSLTGRFLSGRESIAVPETRRPGNGKLLTVTGCAENNLKNLD
                     LSIPLGTFTGVTGVSGSGKATFVNEILFRAVARKLNGTGAVPGKHGALKGVEAIDKVI
                     DIDQSPIGRTPRSNAATYTGLFTFIRDLFAQLPESKARGYQPGRFSFNVKGGRCEACG
                     GDGVVKIEMHFLPDVYVKCDVCRGRRYNRETLEVLYKGKNINEVLEMTVDDALEFLNA
                     IPPVRRKLEILQGVGLGYVHLGQPATTLSGGEAQRIKLATELSKIATGRTLYILDEPT
                     TGLHFHDVRLLLKVLTTLVDRGNTVVVIEHNLDVIKTCDHLIDLGPEGGDGGGRVVAM
                     GTPEEVAATQGSWTGEALKTVLPEAAGVR"
     gene            2944..>3990
                     /locus_tag="VF720_00530"
     CDS             2944..>3990
                     /locus_tag="VF720_00530"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX7877859.1"
                     /translation="MAALTALMAVVNLVSGVHPALAGRLDALHAWLPLEVVHGSRLAT
                     LLAGFALLLLSRALARRKRIAWGLTLVAVIISIAGHLGKGLDWEEATYGLLLIGVLVG
                     YRHEFHALSDGLSVKRALWTTVVAALFVLTYGTLGFFVLDHHFQVNFDPVASIRQTIL
                     MFTSFDNPGLVPRTPFGAWFGGSIYALAGLTGAVALFLLFRPVVARAPATREARERAR
                     RIVARHGRSSIARFTLFDDKSYVFSEGGSMVAYVARGGTALALGDPIGPAEDFRGAIE
                     AFHDEARRHDWTPAFYQVGPDRAAYYREAGFELLPVGREAIVETVDFTLDGKVGAPFR
                     TARNRLERAGHHVSV"
BASE COUNT          671 a         1236 c         1410 g          673 t
ORIGIN      
        1 ccgtcgtatt gacagccccg ctgtcgcccc ctaacgtttc ccttccgcct gacccgcgga
       61 tcctccagtg aaaggcccgt ccccgtccat ggatcgcatc tcgatacgcg gcgcccgcga
      121 acacaatctc aagaacatct cgatcgacat cccccgcgac cgtctcgtcg tgctcaccgg
      181 tgtctccgga agcggcaagt cgagcctggc gttcgacacc atctatgccg aggggcagcg
      241 acgctacgtc gagagcctct ccgcctatgc ccgccagttc ctcgggcaga tggagaagcc
      301 ggacgtcgac cagatcgagg ggctctcgcc cgccatctcg atcgagcagc ggacggccgg
      361 gcggaacccg cgctccaccg tggccaccgt cacggagatc tacgactacc tgcgattgtt
      421 gttcgcgcgc gtcgggatcc agcactgcca caactgcggg cggcggatca gccggatgac
      481 catccaggag atggtggacg tggcgctcaa gcagcctgag ggggcccgcg cgcagctcct
      541 ggcgcccatc gtgaaggggc ggaaggggga gtatcgcgcg gagctcgagc agctcaagaa
      601 gcagggatac ctgcgcgccc gcatcgacgg cgagctgtac gagctggaag agccgccgaa
      661 gctggccaag acgaagaagc acacgatcga aatcgtggtg gatcggttga agatcgatcc
      721 gaagaaccag gggcgactgg ccgacagcct cgagacggcc ctcaagctcg gacaggggca
      781 ggccaccctt tctgtggacg gcgtggcgga ggagatcctc ttcagcgagg cggcctcgtg
      841 cctccactgc ggcctgtcgt tcgacgagct cgagccgcgc atgttctcgt tcaactcgcc
      901 gtacggcgcc tgccccaagt gcgacgggct cggtacgcag atggagatcg acatcgacct
      961 gttgatcccc gacgactcac tgtcgttgcg cgacggggcg ctggccccga tgcgcacggt
     1021 ggaggacggc tggtacggcc agcagatcac cgccctggcc aagaactaca agttttctct
     1081 cgatacgccg ttccggaagc tcacgaagaa gcagcaggaa gtgctgctca acggatcggg
     1141 cgacgaggag atcaccgtcc agtacaagga caagcggtac gacgtcaagt tcaagaagaa
     1201 gttcgagggg gtcatcccga acctcatgcg gcggcaccgc gagacgtcgt cgaactggat
     1261 ccgggagtgg atcgagggtt acatgacgct gcggccgtgc acggcgtgcg acggggcgcg
     1321 gctcaagccg tcgtcgctgg ccgtttcggt gcagggcgac tcgatccgcg acgtgacttc
     1381 ccggtcggtg tcgcgcagcc tcgagtgggc gcgcgcgctg gtgctcacgg aacgcgaggc
     1441 cacgatcgcg acggccatca tgaaggaagt gacgtcgcgc ctcgacttcc tctcggatgt
     1501 cgggctcggc tacctgactt tggatcggac ggccggcacg ctgtcggggg gcgaggcgca
     1561 gcggatccgg ctggccacgc agatcggcag caagctgatg ggcgtactct acatcctcga
     1621 cgagccgtcg atcggcctgc atcatcgcga caaccgcaag ctcctgaata cgcttcatca
     1681 cctgcgcgac ctcggcaaca cggtgatcgt ggtcgagcac gaccaggaga cgatggagga
     1741 agccgacttc ctggtggacc tgggccccgg cgccggcaag cacggcggcg aaatcgtggt
     1801 ggccggccca ccgccggtcg tgcgcgacca tcctgattcg ctgaccgggc gattcctctc
     1861 gggacgcgag tcgattgcgg tgcccgaaac gcgtcggccg gggaacggca agttgctgac
     1921 ggtgacggga tgcgcggaga acaacctcaa gaatctggat ctctccattc cgctgggcac
     1981 gttcaccgga gtcaccggcg tttcgggatc ggggaaagcg acgttcgtga acgagatcct
     2041 cttccgcgcc gtggcccgca agctgaacgg caccggcgcg gtgcccggca agcatggcgc
     2101 gctcaagggc gtggaagcca tcgacaaggt catcgacatc gaccagagcc cgatcgggcg
     2161 cacgccgcgc agcaacgcgg ccacctacac cggactgttc acgttcatcc gcgacctctt
     2221 cgcgcagttg cccgagtcga aggcgcgcgg ctaccagccg gggcgcttca gcttcaacgt
     2281 gaagggtggc cgctgcgagg cgtgcggcgg cgacggcgtg gtgaagatcg agatgcactt
     2341 cctgcccgac gtgtacgtga agtgcgatgt gtgccgcggg cggcgctaca accgcgagac
     2401 gctcgaggtg ctctacaagg ggaagaacat caacgaagtg ctcgagatga ccgtggacga
     2461 cgcgctggag ttcctgaatg ccattccgcc ggtgcggcgg aagctggaga ttctgcaggg
     2521 cgtggggttg gggtacgtgc acctcgggca accggccacc acgctgtctg gtggtgaggc
     2581 gcagcggatc aagctggcca ccgagctttc caagatcgcg accggacgga cgctgtacat
     2641 cctcgatgaa ccgacgaccg ggctgcactt ccatgacgtg cggttgctgc tgaaggtgct
     2701 cacgacgctg gtggatcgcg gcaataccgt cgtggtgatc gaacacaacc tggatgtcat
     2761 caagacgtgc gatcacctga tcgacctcgg gcctgaaggg ggggacgggg gtgggcgcgt
     2821 cgtggcgatg gggacgccgg aggaagtggc ggcgacgcag gggtcgtgga cgggggaggc
     2881 gttgaagacg gtgctgccgg aggcggcggg agtccgctga ggtcagcggc cgcggtccgg
     2941 ctcgtggcgg cgctcaccgc cctgatggcc gtggtcaatc tcgtctccgg tgtgcacccg
     3001 gcgctggccg gtcgactgga cgcgctgcac gcctggcttc cgctcgaggt ggttcacggg
     3061 agccgtctgg ccacgctgct ggcggggttc gcactgctgt tgctctcgcg cgcgctggcc
     3121 cgacggaagc ggatcgcgtg gggcctcacg ctggtggcgg tgatcatttc gatcgccggg
     3181 catctcggga aggggctcga ttgggaggag gccacgtatg gcctcctgct cattggggtg
     3241 ctggtcggct accgccacga gtttcacgcg ctgtcggacg gtctctcggt aaagcgggcg
     3301 ctctggacga cggtggttgc cgcgctcttc gtgctgacct acggcacgct tggattcttt
     3361 gtcctcgacc accacttcca ggtcaatttc gatcctgtcg cgtcgatccg ccagacgatc
     3421 ctgatgttca cctcgttcga caaccccggc ctggtgccgc ggacgccgtt cggcgcgtgg
     3481 ttcggtgggt cgatttacgc gctggcgggg ctgacgggag cggtggcgtt gttcctgttg
     3541 ttccggccgg tggtggcgcg ggcgccggcc acgcgcgagg cgcgggagcg cgcccggcgg
     3601 atcgtggcac ggcacggacg ctcatcgatc gcccgcttca cgctcttcga cgacaagtcg
     3661 tacgtcttca gcgaaggtgg ctcgatggtg gcctacgtgg cgcggggtgg gacggccctg
     3721 gcgctggggg atccgatcgg gccggcggag gactttcggg gagcgattga ggcgtttcat
     3781 gacgaggcgc gccgtcatga ttggacgccg gcgttctacc aggtcgggcc cgatcgggcg
     3841 gcttactacc gggaggccgg gttcgagctt ctgccggtcg gccgtgaagc aattgtcgaa
     3901 acagtcgact tcacgcttga tgggaaggtt ggcgcgccgt ttcggaccgc gcgcaatcga
     3961 ttggagcggg cggggcatca cgtaagcgtg
//
LOCUS       DASVOD010000028         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_679823, whole genome shotgun sequence.
ACCESSION   DASVOD010000028 DASVOD010000000
VERSION     DASVOD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679823"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2890
                     /locus_tag="VF720_00535"
     CDS             <1..2890
                     /locus_tag="VF720_00535"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX7877860.1"
                     /translation="TPNGLARRVAGSGAWVSYQPEAGGLVHPNVWRLLEDKAGGVWAA
                     TPEGISRLDADRLTWQSFRADPNALGRDSVLAIAEDDEGAIWLGTDDGAWRYAPSGDG
                     PTWQHVGVAQGLSSEIVLAIVPDGQRGLWFGGSRGIDHFDGHTWRHYPVTTEGLPLGR
                     VFSLLPDHSGNLWAGTQSNGLFRYDRVDIQGFIAPAGSGDCSARPDLGGHVKPILASN
                     CITGLIEDHAGEIWATTADRGAAHLDITGTWSFPGRAPGRPLSDSLSTLVEDRDGRIW
                     FASGNVSGSGRYSGLAVLEADRVRWQAWRGPGALPSDNVLTLHEDASGAMWAGTTAGV
                     AVHAGGAWRSFLAPAAIGFDVAVYAIEEDESGGIWLRTSDGLYRKGIDAPMPVRFGAA
                     EGLWTDDIRVVHAAGGRVWIGGAGGFGTWTGEDIERSAVTTHDVESIRLMSNGDLWVG
                     TDNGAQRIAPGGAATFYPGEVIGSTPVIGLYEDRRHTAWLATFGGMSRFNGSGWNRFG
                     SSDGLVSDQVARFLEDRRGRLWLSSFSGITQYDPDAVAPQSVFVTTPDALTSSRNVRF
                     IHAAAYGEVADVAFRTRLDGLPSEWTPAVSAEFTGLSDGVHTFEVVGRDWSGNVDPTP
                     ARFTFEVDATPPEALLAAPSFGQAVNGEVTIRGRAADARFQCYEVLARPAGTAGWNDP
                     AVLTLARDCRPVVLGTLAAWNTMALDDGDWEVRLSVEDTLGLAGVAQVSVIVDNHAPF
                     ADVTSPVRVTALDGGDVYTTFEEVHCYFPPRAFDKDAVVTIAPAGGEAWDIAWGEPQL
                     EKPGVIDLGMREGNDSAVLHRSSDGGQSWTLVGGSPDAAGRRLVAIIDAPGRYALLPA
                     GTAPVPSGGLSDPDLIPRVFRPGGGSPDGVSISYRLDGSAPATVRVFNRAGRLVRELA
                     EDAPMGGGVNVLHWDGRDLDGGYVTGGLYLVRVESGGESRVKPLVVLR"
     gene            2965..>3954
                     /locus_tag="VF720_00540"
     CDS             2965..>3954
                     /locus_tag="VF720_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877861.1"
                     /translation="MIVFLVFMVTPAFAVREQVDMGARAIALGGAYSAVADDASAIYW
                     NPAGLCQLGHQELVASHADLYGEGIKQNVVGAVVPLTPDQAAAFEWYSTGYDDGELDF
                     GENRLDLAFSLRPNRRVAFGITGKYLSRNVDLDETAFGSGHGLGMDAGVLAFVARNVR
                     VGAVLQDVFDTHIDDPDRGSTVAYPRGLRVAGAWTPAAPLTLALDVDDRLHGGVEYAP
                     VEVLALRAGAQDDLGGPEGTTWSAGAGLRIGIVKFDYAVDFHPDLGSTNHFDLGLAFN
                     FNPAQVRIEKVEGQEVYSSLYKSYDRRPLAKVKLRNLKDVPLTARVVLSSPDLM"
BASE COUNT          565 a         1368 c         1426 g          595 t
ORIGIN      
        1 cacgccgaac ggacttgccc ggcgggtggc cgggtccggc gcgtgggttt cctaccagcc
       61 ggaggccggc ggcctcgtgc accccaacgt gtggcggttg ctcgaggaca aagcgggggg
      121 cgtgtgggcc gccacgccgg aagggatcag ccgcctcgac gcggaccgcc tcacctggca
      181 gagctttcgc gccgatccga acgcgctggg gcgcgacagc gtgctggcca tcgccgagga
      241 tgacgaaggc gccatctggc tcggcaccga tgacggggcc tggcgctacg caccgtccgg
      301 cgatgggccc acctggcaac acgtcggcgt cgcgcagggg ctttccagcg agatcgtcct
      361 cgccatcgtc cccgacggcc agcgagggct ctggttcggc ggctcgcgcg gcatcgatca
      421 ttttgacggc cacacctggc ggcactaccc ggtcacgaca gagggactgc cgctcgggcg
      481 cgttttctcg ctgctccccg atcactcggg caacctgtgg gcggggacgc agtccaacgg
      541 cctcttccgc tacgaccgcg tcgacatcca ggggttcatc gcaccggccg gcagcggcga
      601 ctgctcggcg cgtccggatc tcggcggcca cgtcaaaccg atcctggcca gcaactgcat
      661 caccgggctg atcgaggacc atgccggcga aatatgggcc accaccgccg atcgcggtgc
      721 cgcgcacctc gacatcaccg gcacgtggag ctttccgggt cgtgcgcccg gacgccccct
      781 ctccgattcg ctgagcacgc tggtggagga ccgcgacgga cggatctggt tcgccagcgg
      841 caacgtgtcg ggaagcgggc gctacagcgg gctggccgtg ctcgaggccg accgcgtgcg
      901 gtggcaggcg tggcgcgggc ccggggcatt accgagcgac aacgtcctca cgctgcacga
      961 agacgcgagc ggcgcgatgt gggccggcac gacagccggc gtggcggtgc atgcgggggg
     1021 tgcctggcga tcgttcctcg caccggccgc gatcggcttc gacgtggcgg tgtacgccat
     1081 cgaggaagac gaatcgggcg gcatctggct ccgcacgtcg gacggcctgt accgcaaggg
     1141 gatcgatgcc ccgatgccgg tgcggttcgg cgcagccgag gggctctgga ccgatgacat
     1201 ccgcgtggtg cacgcggcgg gggggcgcgt ctggatcggc ggcgccggcg gcttcggtac
     1261 gtggacgggc gaggacatcg agaggtcggc ggtcacgacg cacgacgtgg aatcgatccg
     1321 cctcatgtcg aacggcgatc tctgggtcgg aaccgacaac ggcgcccaga ggatcgcgcc
     1381 gggcggcgcc gctaccttct atccgggcga ggtgatcggc tcgacgccgg tgatcggcct
     1441 gtacgaagac cgccggcata ccgcctggct ggccacgttc ggcggcatga gccgcttcaa
     1501 cggctcgggg tggaaccgct tcggcagcag cgacggcctc gtctccgacc aggtggcccg
     1561 tttcctcgag gaccggcggg gacgactctg gctgtcgagc ttctccggca tcacgcagta
     1621 cgacccggat gccgtggcgc cgcagtccgt gttcgtgacc acgccggacg cgctcacctc
     1681 gagccgcaac gtccgcttca tccacgccgc cgcctacggc gaggtggccg acgtggcctt
     1741 ccgcacccgg ctcgacggcc tgccgtccga gtggacgccg gcggtgagcg ccgagttcac
     1801 cggcctctcc gacggggtac acaccttcga ggtcgtcggg cgcgactgga gcggcaacgt
     1861 cgatccgacg cccgcccgct tcaccttcga ggtggatgcc acgccgccgg aagccttgct
     1921 ggccgcgccg tcgttcggcc aggccgtgaa cggcgaggtg acgatccgcg gccgcgccgc
     1981 ggacgcccgc ttccaatgtt acgaagtcct cgcgcgcccg gccggcacgg ccggctggaa
     2041 cgacccggct gtcctcacgc tggcgcgtga ttgccgcccg gtcgtacttg gcaccctggc
     2101 cgcctggaac accatggcgc tcgatgacgg cgactgggaa gtgcgccttt ccgtggaaga
     2161 cacgctcggc ctggccggcg tggcgcaggt gtcggtgatc gtggacaacc acgcgccgtt
     2221 cgccgatgtc acctcgccgg tgcgcgtgac cgcgctcgac gggggagatg tctacaccac
     2281 cttcgaggaa gtgcactgct actttccgcc gcgcgccttc gacaaggacg ccgtcgtcac
     2341 gatcgcgccg gccggtggcg aggcgtggga catcgcgtgg ggcgagccgc aactggaaaa
     2401 gccgggcgtc atcgacctcg gcatgaggga gggcaacgac tccgccgtcc tgcaccgatc
     2461 gagcgacggc ggccagagct ggaccctcgt gggcggatcg cccgacgccg ccggccggcg
     2521 cctcgtggcc atcatcgacg cccccgggcg ctacgcgctc ctgccggccg ggacggcgcc
     2581 ggtgccctcg ggcggcctct cggatcccga tctcatcccg cgcgtcttcc ggcccggcgg
     2641 cggatcgccg gacggtgtgt cgatcagcta ccggctcgat ggctcggcgc ccgcgacggt
     2701 gcgcgtcttc aaccgggccg gccggctggt ccgggaactg gcggaggacg ccccgatggg
     2761 ggggggcgtg aacgtgctgc actgggacgg gcgcgacctg gacggcggat acgtgaccgg
     2821 cgggctctac ctcgtccggg tggagtcggg tggcgaaagc cgtgtgaagc cgctcgtggt
     2881 gctgcgatga attggacggt gattcggccg gggtggccgc cgggacgcga cggtgcaccg
     2941 agacagtcgg gcaggctggt ttcggtgatc gtgttcctgg tattcatggt caccccggct
     3001 tttgccgtcc gtgaacaggt ggacatgggg gcgcgggcca tcgccctggg tggcgcctac
     3061 tcggcggtgg ccgacgatgc ctcggccatc tactggaacc cggccgggct ctgccagctg
     3121 ggccaccagg aactcgtggc ctcgcacgcc gatctctacg gcgaggggat caagcagaac
     3181 gtggtcgggg ccgtggtgcc cctcacgccc gaccaggcgg cggccttcga gtggtacagc
     3241 accggctacg atgacggcga gctcgacttc ggcgagaacc ggctcgacct ggcattctcg
     3301 ctgcggccga acaggcgcgt ggcgttcggc atcaccggca agtacctgtc acgcaatgtc
     3361 gacctcgacg aaacggcctt cggcagcggc cacgggctcg gcatggatgc cggcgtgctg
     3421 gccttcgtgg cgcgcaacgt gcgtgtgggc gccgtattac aagacgtctt cgacacgcac
     3481 atcgatgatc ccgaccgtgg ctccacggtg gcctacccgc gtggcctgcg cgtggccggg
     3541 gcatggaccc cggccgcgcc gctcaccctg gcgctcgacg tggacgaccg gctccacggc
     3601 ggcgtggagt acgcacccgt cgaggtcctg gccctgcgcg ccggtgcgca ggacgacctg
     3661 gggggccccg agggaaccac ctggagcgcc ggtgccggcc tcaggatcgg catcgtcaag
     3721 ttcgactacg ccgtcgactt ccatcccgac ctcgggtcga ccaaccactt cgacctgggg
     3781 ctcgccttca atttcaatcc ggcccaggtg cgcatcgaga aggtggaagg gcaggaggtg
     3841 tacagctcgc tctacaagag ctacgaccgc cgcccgctgg ccaaggtcaa gctccgcaat
     3901 ctcaaggatg tgccgctgac cgcacgcgtg gtgctgtcgt cgcccgacct catg
//
LOCUS       DASVOD010000029         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_784174, whole genome shotgun sequence.
ACCESSION   DASVOD010000029 DASVOD010000000
VERSION     DASVOD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784174"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VF720_00545"
     CDS             <1..781
                     /locus_tag="VF720_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7877862.1"
                     /translation="ILFAEPTGLFPRVAEPLAENLGILCGHVANAAAGASGPTPGQPY
                     DIGFAFDPDVDRLSLVDETGRALGEEYTLALAARFVLERKPGPAVTNLSTSAMIDAVA
                     ARLGRTVLRTAVGEANVVEEMARAGAPIGGEGNGGVILPAVHLGRDAPVGVALVLGLM
                     AATGKKLSELAAELPTMAMVKAKQELAAPPDLARLEDPLARALPGGILDRRDGLRVSR
                     EGEWIHVRKSGTEPIVRIIAEAGDMTRAGQLRDLALEALGS"
     gene            890..2716
                     /gene="glmS"
                     /locus_tag="VF720_00550"
     CDS             890..2716
                     /gene="glmS"
                     /locus_tag="VF720_00550"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX7877863.1"
                     /translation="MCGIVGYVGPRDCVDLLIDGLKRLEYRGYDSAGIAIQNGKGLEI
                     HKAAGKIKSLEERVNGARPTGTSGIAHTRWATHGEPNDTNAHPHVSQNGRIAVAHNGI
                     IENHAALRASLVQKGYAFTTETDTEVLAHLIEEYYDGNLEQAVIRSLQHVDGTYGIAV
                     ICSDEPGKVVGARKGSPLVLGLGQGEYFIASDVSAILRHTKQVIYLDDGEVAVITPNE
                     YRTMTIDNQPVDKTVSEVEWELEQIEKGGYDHFMLKEIIEQPESVRNAFRGRLLLEAG
                     TGHLGGLNMEKNALRLIDRIIIIGCGTSWHAGLIGEYMIEEYAQIPVEVEYASEFRYR
                     NPVVKPGTVVFVISQSGETIDTLEAMREAQRKGAPALGIVNVVGSTIARESDGGTYIH
                     AGPEIGVASTKAFTSQVTALAVLTLILGRLRGMSLEQGRQVVTELNAIPGKIAKILET
                     NDAIREIARQLVQHDNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKHGPIA
                     LIDENMPVVFICTRDAAYEKIMSNMEEVRARKGKIIAVATEGDEDVRKKAHHVIYVPE
                     TIQMLTPLLSVIPLQLLAYHIAVLRGEHVDQPRNLAKSVTVE"
     gene            2777..3178
                     /locus_tag="VF720_00555"
     CDS             2777..3178
                     /locus_tag="VF720_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HEX7877864.1"
                     /translation="MRKIVQTDKAPGAIGPYSQGIEYSVSGPIRIVYTAGQVGLDPVT
                     GKLVDGDVAAQTAQVFRNLEGVLGARGFHLADAVKVSVFLTDMANFGAMNEVYAAHFP
                     AHPPARSTVAVSALPAGALVEIDVVAIKPGL"
     gene            3316..3413
                     /locus_tag="VF720_00560"
     tRNA            3316..3413
                     /locus_tag="VF720_00560"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3350..3352,aa:Sec,seq:tca)
     gene            3433..>4084
                     /locus_tag="VF720_00565"
     CDS             3433..>4084
                     /locus_tag="VF720_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877865.1"
                     /translation="MRPTLLTFGWLAFCWLSVAGPVVREAKGGGRFDSRESFTHSPLL
                     TAAMRTAADMSSDSTATSPIAIVPIDSLGKAFKIADSAATDASRDSTGNQSVGSTPAP
                     RKIDKPPGEAWTTIRSGLIPGWGQLVNRKPLKAGLFFGAWAFLGAQAIVAESDRREAQ
                     EAFDESGSEADMIAVNDAVDQRNSRLWWMGGVAVFAMLDAYVDVHFWGFEEQWQARV"
BASE COUNT          708 a         1328 c         1391 g          657 t
ORIGIN      
        1 gatccttttc gcggagccga ccgggctctt cccccgtgtg gcggaaccgc tggccgaaaa
       61 tctcggcatc ctgtgcggcc acgtggcgaa cgcggcggcg ggtgcctcgg gtccgactcc
      121 cggccagccc tacgacatcg gctttgcctt cgacccggac gtggatcgcc tctccctggt
      181 cgatgaaacc gggcgcgccc tgggtgagga atacacgctg gccctggccg cgcgcttcgt
      241 gctggaacgg aagcccggcc cggccgtcac gaatctctcc acttcggcca tgatcgacgc
      301 cgtggcggcc cggctcgggc ggaccgtcct ccggacggcg gtcggtgagg ccaacgtcgt
      361 cgaggagatg gcgcgggccg gtgcgccgat cggcggggaa gggaacggtg gggtgatcct
      421 cccggcggtg cacctcgggc gggatgcccc ggtcggggtg gccctcgtcc tggggctcat
      481 ggccgccact ggaaagaagc tctccgaact cgccgccgaa ctgccgacaa tggccatggt
      541 gaaggcgaaa caggaactgg cggcgccacc cgacctggca cgcctcgagg atccgctcgc
      601 ccgggccctg ccgggtggta tcctcgaccg gcgtgacggc ctccgcgtct cccgcgaggg
      661 agagtggatc catgtccgga agtccggcac cgagccgatc gtccggatca tcgccgaagc
      721 gggtgatatg acccgggccg gccagttgcg cgacctggcc ctcgaagcgc tggggtcctg
      781 aaacaaggag cgatctcccc agaccggggg ggccatcgat ccagacagcg gggcgatctc
      841 cccacagact gaaggggctg gtttcccctc accggaggaa cggaacggca tgtgtggaat
      901 cgtcggctac gtcggcccgc gcgattgcgt ggatctcctc atcgacggcc tgaagcgact
      961 cgagtaccgc ggctacgact ccgccggcat cgccatccag aacggcaagg gcctggaaat
     1021 ccacaaggcc gccggcaaaa tcaagagcct cgaggaacgc gtgaacggcg cccggccgac
     1081 gggcacctcg ggcatcgccc acacccgctg ggccacccac ggtgagccga acgacaccaa
     1141 cgcccacccc cacgtcagcc agaacggccg catcgcggtg gcccacaacg gcatcatcga
     1201 gaatcatgcc gccctgcggg ccagcctggt ccagaaggga tacgccttca cgaccgagac
     1261 cgataccgag gtgctggcgc acctgatcga ggaatactac gacggcaacc tggagcaggc
     1321 cgtcatccgc tcgctccagc acgtggacgg cacgtacggc atcgccgtca tctgctccga
     1381 cgagcccggc aaggtggtcg gcgcccggaa gggtagcccg ctcgtcctcg gcctcgggca
     1441 gggggagtac ttcatcgcct ccgacgtgtc ggccatcctg cggcacacca agcaggtcat
     1501 ctacctcgat gacggcgaag tggcggtcat cacgcccaac gagtaccgca cgatgaccat
     1561 cgacaaccag ccggtcgaca agacggtgtc cgaggtggag tgggagctcg agcagatcga
     1621 gaagggcggc tacgaccact tcatgctgaa ggagatcatc gagcagcccg agtcggtgcg
     1681 gaacgccttc cgcggccggc tgctgctgga ggccggcacg ggacaccttg gcggcctgaa
     1741 catggagaag aacgcgctcc ggttgatcga ccggatcatc atcatcggct gcggcacgtc
     1801 atggcacgcc ggactgatcg gcgaatacat gatcgaggag tacgcccaga tcccggtcga
     1861 ggtggagtac gcgtcggagt tccgctaccg gaacccggtg gtgaagcccg gcacggtggt
     1921 gttcgtgatc tcccagtcgg gcgagacgat cgacacgctc gaggccatgc gcgaggccca
     1981 gcgcaagggc gccccggcgc tcgggatcgt gaacgtggtc ggctcgacca tcgcccgcga
     2041 gtcggacggg ggcacgtaca tccatgccgg gccggagatc ggcgtggcgt cgaccaaggc
     2101 gttcacgtcg caggtcacgg cgctggcggt gctcacgttg atccttggcc gcctccgcgg
     2161 catgagcctc gagcagggac gccaggtggt caccgagctg aacgccatcc cggggaagat
     2221 cgccaagatc ctcgagacga acgacgccat ccgcgagatc gcccggcaac tggtgcagca
     2281 tgacaacttt ctctacctgg ggcgcggcta caactttccg gtggcgctcg aaggtgccct
     2341 gaaattgaaa gagatttcgt acatccacgc cgagggttac cccgcggcgg agatgaagca
     2401 cgggccgatc gcactgatcg acgagaacat gccggtggtc ttcatctgca cccgcgacgc
     2461 ggcgtacgag aagatcatga gcaacatgga ggaggtgcgc gcgcggaagg ggaagatcat
     2521 cgccgtggcc accgaggggg acgaggacgt gcggaagaag gcgcaccacg tcatctacgt
     2581 tcccgaaacg atccagatgc tcacgccgct cctctcggtc atcccgttgc agctgctggc
     2641 gtatcacatc gctgtgctgc gcggcgagca cgtcgaccag ccgcgcaacc tggccaagag
     2701 cgttacggtc gagtagcggg cacccatccc ctcgcgttga accggcgccg gggccggctc
     2761 ccggcaagga gtcgacatgc gcaagatcgt ccagaccgac aaggcgcccg gggccatcgg
     2821 cccctactcg caggggatcg agtactccgt gtccgggccc atccggatcg tgtataccgc
     2881 cggccaggtc ggactcgatc cggttaccgg caagctggtc gatggcgatg tggctgccca
     2941 gacggcccag gtcttccgca acctcgaagg tgtcctcggc gcccgaggct ttcacctggc
     3001 cgatgcggtc aaggtctcgg tcttcctgac cgacatggcc aatttcggcg cgatgaacga
     3061 ggtgtacgcc gcccacttcc cggcccaccc gccggcccgc tcgacggtgg ccgtgtcggc
     3121 gcttccggcg ggggcgctgg tcgagatcga cgtggtggcg atcaagccgg ggctgtaggg
     3181 ggccgagata ggcgggcttc gttcccgggc cgggtccctc atgtcgcgcc gtttcgcccc
     3241 ccgaagttgc tttcgttccc gccggttgag gcgcacgcgt aaccccgcta caatcttcgg
     3301 cttgtgcaac gtaaagggag cgaacgagtc ctggtgggct ccgaggactt caaatccttt
     3361 tgccgggcgc taatcacgtc cggggtgggt tcgattccca cacgttcccg ccagtactgg
     3421 ctggcgcgcg acgtgcggcc gaccctgctt accttcggat ggctggcgtt ttgctggctg
     3481 tccgttgccg gccccgttgt tcgtgaagcg aagggcggtg gccgtttcga ttcccgggag
     3541 tcattcactc acagcccttt gttgaccgcg gccatgcgga cggcggcaga tatgtcgtcc
     3601 gactcgacgg cgacttcccc gatagcgatc gtgccgatcg acagtctcgg aaaagcgttc
     3661 aagattgctg attcggcggc gaccgacgcg agtcgcgatt caaccgggaa tcagtcggtt
     3721 gggtcgaccc ccgcgccaag gaagatcgac aagccgcctg gcgaggcgtg gacgaccata
     3781 cgctccgggc ttatccccgg ctgggggcag ttggtcaatc gcaaaccgct gaaggccggc
     3841 ctctttttcg gggcctgggc gttcctgggc gcgcaggcga tcgtagcgga gtcggatcgt
     3901 cgggaggccc aggaagcgtt cgacgagtcc ggatcggagg ccgacatgat tgcggtcaac
     3961 gatgcggtcg accagcggaa ttcacgcctc tggtggatgg ggggcgtggc ggtcttcgcg
     4021 atgcttgatg cgtacgtgga cgtgcatttc tggggttttg aagagcagtg gcaggcgcgt
     4081 gtgg
//
LOCUS       DASVOD010000030         9587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_366846, whole genome shotgun sequence.
ACCESSION   DASVOD010000030 DASVOD010000000
VERSION     DASVOD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366846"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VF720_00570"
     CDS             complement(<1..696)
                     /locus_tag="VF720_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7877866.1"
                     /translation="MLIVMTPTATPEDIARVVGAIEALGFKPNPIPGAVRTAIGITGN
                     QGPVDPRVFEALPGVQEAIPVSKPFKLVSRDWKAADTIVTVGGAKIGGGHWAVMAGPC
                     GVESMDQALAIAERVKKAGAQVFRAGAFKPRTSPYSFQGLGEEGLKILARVRSETGLP
                     IVTEAIDADTVSAVEEYADMIQVGARNMQAFTLLKRAGKSKLPVLLKRGLSATVDELL
                     LAAEYIMSEGNPNV"
     gene            complement(690..1514)
                     /gene="trpA"
                     /locus_tag="VF720_00575"
     CDS             complement(690..1514)
                     /gene="trpA"
                     /locus_tag="VF720_00575"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX7877867.1"
                     /translation="MSAATARLARAIERARGEARPLVIPYLTVGFPTLGETVDLAMAA
                     AAAGVDALELGLPFSDPLADGPVIQAASQAALAGGVTHERAFEIAREVRRRSELPLLF
                     MGYANPMYHYGLEKFIHVMSEAGVDGLIVPDLPHEEAGAAREAAGRAGVSWVPLIAPT
                     TPTTRIPLLDAAATDFTYCVALTGVTGVRADLDPELPAYLERVAGAVSRPFVVGFGIS
                     KPEQVARVAPPAAGVVIGSALIRAIGAATTPEERRGAVTDFLAPFRKQSRTGGSSC"
     gene            complement(1511..2716)
                     /gene="trpB"
                     /locus_tag="VF720_00580"
     CDS             complement(1511..2716)
                     /gene="trpB"
                     /locus_tag="VF720_00580"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018937748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX7877868.1"
                     /translation="MSGMDQRPDARGYFGAYGGRFVPETLMAALESLAAAYETARCDP
                     DFVAALSSELHRFAGRPTPLDPAPRLGEAWGGICLHLKREDLLHTGAHKINNTIGQIL
                     LARRMGKTRIIAETGAGQHGVATATVAAKFGLPCVVYMGEEDIKRQSLNYFRMKLLGA
                     EVVPVSSGSKTLKDATNEAIRDWVTNVRDTFYIIGSVVGPHPYPVMVRDFQSVIGTEA
                     RAQHLEVTGHLPDVAVACVGGGSNAIGLFHAFVPDESVRLVGVEAGGHGVDTDQHSAT
                     LTKGEPGVLHGARSYLLQDQDGQVRLAHSVSAGLDYPGVGPEHAYLKDSARASYATVT
                     DDEALEALQEVARLTGIVPALETAHAFAWLKREGPALGKGTRVVLGLSGRGDKDVPRL
                     ADLLPEAKR"
     gene            complement(2713..3354)
                     /locus_tag="VF720_00585"
     CDS             complement(2713..3354)
                     /locus_tag="VF720_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX7877869.1"
                     /translation="MPFRVRIKVCGLTLPDNARAVAELGVDEIGLNFFEPSTRYVDPV
                     AARDIARTLPPEVRRVGVFVNASGAEIDRIAELVGLDRVQLHGEPSRDFCLARTRPVV
                     RAFRSAPELTLEELQEFRDLPVLLDGFHPGLHGGTGRLADWELAARIAAAGFELYLAG
                     GLGPDNLSEAIARVSPSVVDLNSGVESAPGVKDLGKVQAAIAVIRGAVMERDR"
     gene            complement(3354..4856)
                     /gene="trpE"
                     /locus_tag="VF720_00590"
     CDS             complement(3354..4856)
                     /gene="trpE"
                     /locus_tag="VF720_00590"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEX7877870.1"
                     /translation="MLENPGPEGLRERSANGNLVPVHREIAADLMTPVAAFLRIDGPN
                     TESFLLESVEGGERLARYSFLGRDPFLAVTIHGRYVQVVDHRAGTTRIFEARDPRAAL
                     RELLAPYRAARVGGLPRFTGGAVGWFGYDTVRWIEELPERARDDRGLADVELRLFDTV
                     LAFDHARGRLVLIANAHVGDAVTPEHFEAAWADAVARLDRIESMLTTELPATPAARAP
                     EAPAAPNSNMRREEFEAMVTTALEHIRAGDIFQVVLSQRFEVPTTARPFDLYRSLRTV
                     NPSPYMYFLNYGRAPVVGSSPEPLVRLHDGRLEYRPIAGTCPRGADEEEDARNAAALA
                     GDDKERAEHIMLVDLGRNDLGRVAEPGSVVVSDLMIVESYSHVMHLVSRLTARLRAGC
                     DALDALFACFPAGTVSGAPKVRAMEIIEALEPTRRGLYAGAVGYLDFAGNLDTAIALR
                     TMVVEDGVAYIQAGAGIVADSKPAREYEETRQKARALVTAIERAGSRGLA"
     gene            complement(4850..6355)
                     /locus_tag="VF720_00595"
     CDS             complement(4850..6355)
                     /locus_tag="VF720_00595"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEX7877871.1"
                     /translation="MKSERNLVLVVAAADREKARRALAAPPDGIDMLELRIDRLAPEE
                     RTAPVVADLVRAAALPVIATCRPIDESGFHDGPDADRWALLSAALGAGARLVDIEWSR
                     IRRDHGLAARWPADRLLVSHHAGADAGHAIEDELLAMTRVEAAGVKLVATPASFTESM
                     RLVDLTRHLVRSGRRVTCFSAGAAALAGRVLGYLAGAWLTYLVPDDETGLPPSIPHVS
                     ELITMYRLPAQGQSVKLLGLAGHPLGHSLSPRMQNGVIRVLGERYLMLPLESPSIAEV
                     VDYVRHNDIRGLAVTRPHKETIVPLLDGLDATARSAGAVNTVVRDHDRLVGWNTDLLA
                     MRTILREWSAGPDDRAVVLGAGGAARAVVAALAERQVPVTILNRDTKRARLLADKFAG
                     VTVAGKPDALDAVTGTIFIQCTPLGAAGETVRLPGWLGAGGRLLDLAYRDGMTPLESA
                     AHAAGHRVVGGHDFLARQGAAQFALWTGRLVNADLFRDALGTNLVTREPAC"
     gene            complement(6352..7203)
                     /gene="larE"
                     /locus_tag="VF720_00600"
     CDS             complement(6352..7203)
                     /gene="larE"
                     /locus_tag="VF720_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEX7877872.1"
                     /translation="MASDRVVPVIAVREGLEGRAARLEAILADMGSVLVAYSGGVDSA
                     YLLLAAHRVLGSGAAGLLAVSPSLPAAEHEAALQVAAEIGARLEVVEAFEHMNPEYVA
                     NNPNRCYFCKTELFDVAGPVAERLGLAHVAYGANRDDLGDFRPGMQAARERAIRAPLL
                     EADLDKADIRLLAREAGLSIWDKPAFACLASRIPHGTPVTTERLARVERAEVVLREAG
                     FRQFRVRDLGRCARVEVDPVDVSRLLSEPWRGRVTAGLLGAGFDDVEIDPEGYRPGKL
                     HGTKGVA"
     gene            complement(7193..8137)
                     /gene="cysK"
                     /locus_tag="VF720_00605"
     CDS             complement(7193..8137)
                     /gene="cysK"
                     /locus_tag="VF720_00605"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018034991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX7877873.1"
                     /translation="MAPIHDNLTDLIGRTPLVRLNRVTAGLPAEIIVKLESMNPMSSV
                     KDRTGWAMIQAAEKVGRLAPGSTIIEPTSGNTGVALAFVSAARGYKLVLAMPDTMSEG
                     RVKLLAALGARVILTPGALGMKGAVEEAMALAERTPGAFVPMQFENPANPDVHRRTTA
                     EEIWSDTEGRLDAFVAGVGTGGTITGVGEVLKARLPRVRIVGVEPAESPVLSGGEGAP
                     HDIQGIGAGFVPPVLNRAILDEVMTVSGADAMRMTRRLAREEGLLVGISSGAAVTASM
                     RLAERPEMNGRRIVTLLASHGERYLGVPGLFEGDRHGE"
     gene            complement(8186..8620)
                     /locus_tag="VF720_00610"
     CDS             complement(8186..8620)
                     /locus_tag="VF720_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HEX7877874.1"
                     /translation="MRLTSRIDYGLRAMLDLALHAGGGPVPNHDISRRQRIPEQYLKQ
                     ILPALRRQGLVVSTRGPNGGHRLARRPDEVTVAEILKALEGRLDVTGFGKEAGAQGES
                     SRILAQFWGELSQAMENVLEGTTLDDLCRKKRRHDQSLIYQI"
     gene            complement(8707..>9587)
                     /locus_tag="VF720_00615"
     CDS             complement(8707..>9587)
                     /locus_tag="VF720_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877875.1"
                     /translation="ARGGPERTCLAGAHAMSDIVLVRGGGRLGTATAHRLRLCGFPVV
                     IAEAARPTLIHRRAAFGAAIEDSEVSVEGVRGRRASGIEGIRQVLDEGGVPVLVDPAA
                     TIREGLAPWVLVDAIGLEWNVGTRREDAPIVVAMGPGFSAGADAHAVVETRPNVDLGR
                     FTTEGPLREAPLSLAAAGAVDRGLVRAPIPGRFESTASIGISVEVDAVLGTVGGREVR
                     APIAGLVIGLLAGGLFVGPGMRVAEIDPGADAERVSAIDPLARAAAGGVLEAILHLAA
                     LGREGGQSLESRRTGD"
BASE COUNT         1457 a         3465 c         3278 g         1387 t
ORIGIN      
        1 cacgttcggg ttcccctcgc tcatgatgta ctcggcggcc aggaggagtt catcgacggt
       61 ggccgaaagg ccccgcttga ggagcaccgg gagcttcgac ttgccggccc gcttcaggag
      121 ggtgaaggcc tgcatgttgc gcgcgccgac ctggatcatg tcggcgtact cctccaccgc
      181 cgacacggtg tcggcgtcga tcgcctcggt cacgatcggc aggcccgtct cgctccgcac
      241 cctggccagg atcttgagac cttcctctcc gagcccctgg aaggaatagg gactggtgcg
      301 cggcttgaag gcgcccgccc ggaagacctg agcgccggcc ttcttcaccc gctcggcgat
      361 cgcgagcgcc tggtccatcg actccacgcc gcacggcccg gccatcacgg cccaatggcc
      421 gccgccgatc ttcgccccac ccaccgtcac gatcgtatcg gccgccttcc aatcccggct
      481 gaccagcttg aacggcttcg aaaccgggat ggcttcctgc acgccgggaa gcgcctcgaa
      541 gacgcgcggg tccacaggtc cctggttgcc ggtgatcccg atggccgtgc gcaccgcgcc
      601 gggaatcggg ttcggcttga aaccaagggc ttcgatggcc ccgaccaccc gcgcgatgtc
      661 ttcgggcgtg gcggtgggcg tcatgacgat cagcacgaag aacctccggt tcgggactgt
      721 tttcgaaatg gcgccaggaa atcggtgacg gcgccccgcc gctcttccgg ggtggtcgcc
      781 gcgccgatcg cccggatcag ggcactgccg atgacgactc ccgccgccgg cggcgccacc
      841 ctggccacct gctccggctt cgagatgccg aacccgacta cgaagggtcg ggaaacggca
      901 ccggccaccc gttcgaggta ggccggcagc tccgggtcga gatcggcgcg cacacccgtg
      961 acgccggtga gcgcgacaca gtacgtgaaa tcggtggcgg ctgcatccag cagcgggatg
     1021 cgcgtggtgg gtgtcgtcgg ggcaatgagc ggcacccagg acacgccggc acgcccggcc
     1081 gcctcgcggg ccgcgcccgc ctcctcgtgc ggcaggtccg gcacgatcaa cccgtccaca
     1141 ccggcttcgg acatcacgtg aatgaacttt tccaaaccat agtgatacat cgggttggcg
     1201 taacccatga agaggagcgg cagctcggaa cgccggcgga cttcgcgggc gatttcgaag
     1261 gcccgctcgt gcgtgacccc gccggccaac gccgcttgcg aggcggcctg gatgaccggg
     1321 ccgtcggcca acgggtccga gaacggcagg ccgagctcga gggcatccac ccctgccgcc
     1381 gcggctgcca tcgcgaggtc caccgtttcc ccgagcgtcg gaaagccgac cgtgagatag
     1441 gggatgacga gcggccgggc ctcgccccgg gccctctcga tggcgcgggc cagccgggcg
     1501 gtcgcggcgc tcatcgcttt gcctccggaa ggagatcggc gagtcgcggc acgtccttgt
     1561 cgccccgccc cgagaggccg aggaccacgc gcgtaccctt gccgagcgcc ggaccttcgc
     1621 gcttcagcca ggcgaaggcg tgggcggttt cgagcgccgg cacgatcccg gtcagtcggg
     1681 ccacttcctg cagcgcttcg agcgcctcgt cgtcggtgac ggtggcgtag ctcgcccgcg
     1741 cggaatcctt gaggtacgcg tgctcgggcc cgacgcccgg atagtcgagt ccggcggaaa
     1801 cggagtgggc cagccgcacc tggccgtctt gatcctgtag aaggtacgag cgggcgccgt
     1861 gcaggacgcc gggctcccct ttcgtgagcg tggccgagtg ctggtcggtg tccacgccat
     1921 gcccccccgc ctccacgccg acaaggcgca ccgactcatc gggcacaaac gcatggaaga
     1981 ggccgatggc attgctgccg ccccccacgc aggccaccgc cacgtcgggg agatggccgg
     2041 tgacttcgag gtgctgggcg cgtgcctccg tcccgatgac cgactggaaa tcgcgcacca
     2101 tgaccggata ggggtgaggt ccgacgaccg atccgatgat gtagaaggtg tcgcgcacgt
     2161 tcgtcaccca gtcgcggatg gcttcgttgg tggcgtcctt gagcgttttg ctcccgctcg
     2221 acaccgggac gacctcggcg ccgaggagct tcatccggaa atagttcagc gactgccgct
     2281 tgatgtcctc ctcgcccatg tacaccacgc acggcaggcc gaacttggcg gccaccgtgg
     2341 cggtggccac gccatgctga ccggcgcccg tctcggcgat gatgcgcgtc ttccccatcc
     2401 ggcgggccag gaggatctgg ccgatcgtgt tgttgatctt gtgcgcgccc gtatgaagga
     2461 ggtcctcccg cttgaggtgc aggcaaatcc ctccccacgc ctcaccaagg cgcggcgccg
     2521 ggtcgagcgg cgtgggccgg ccggcgaagc ggtgcagctc cgacgaaagc gcggccacga
     2581 aatccggatc gcagcgcgcc gtctcgtacg cggcagccag cgattcgagc gcggccatca
     2641 gcgtctcggg tacgaatcgc ccgccgtagg ccccgaagta gccgcgcgcg tcggggcgct
     2701 ggtccattcc gctcatcggt cccgctccat gacggcgccc cggatgacgg cgatggccgc
     2761 ctgcaccttg ccgaggtcct tcacccccgg cgccgactcg acgccgctgt tcaggtcgac
     2821 cacgctcggt gacacccggg cgatggcctc ggacaggttg tccggcccga ggccgccggc
     2881 gaggtagagc tcgaagccgg cggcggcgat acgcgccgcc agctcccagt cggccaggcg
     2941 acccgtgccg ccgtgcaacc cgggatggaa tccgtcgagg aggaccggga gatcgcgaaa
     3001 ctcctgcagt tcctccagcg tgagctcggg cgccgaccgg aaggcgcgca cgaccggccg
     3061 cgtgcgcgcc aggcagaagt cgcggctcgg ttcgccgtgg agctgcacgc ggtcgaggcc
     3121 gacgagttcg gcgatccggt cgatctcggc cccggacgcg ttgacgaaca cgccaacccg
     3181 ccgcacctcc ggcggcagcg tccgcgcgat gtcgcgcgcg gcaaccggat cgacgtagcg
     3241 ggtgctcggc tcgaagaagt tgaggccgat ctcgtccaca cccagctcgg ccacggcccg
     3301 ggcgttgtcg gggagcgtca gcccgcacac cttgatccgc acgcggaaag gcatcaggcc
     3361 agcccccggc ttccggcacg ctcgatggcc gtcacgagcg cccgcgcctt ctgccgcgtc
     3421 tcctcgtact cgcgggccgg cttgctgtcg gccacgatgc cggcgccggc ctggatgtag
     3481 gccacgccat cctcaaccac catcgtgcgg agcgcgatcg ccgtgtcgag gttgccggca
     3541 aagtcgaggt agccgacggc acccgcgtag agcccacgcc gggtcggttc gagcgcctcg
     3601 atgatctcca tcgcccggac cttgggggcg ccggataccg tgcccgcggg aaagcaggcg
     3661 aagagggcgt cgagcgcgtc gcacccggcg cggaggcggg ccgtcaggcg cgaaacgagg
     3721 tgcatgacat gcgagtacga ctcgacgatc atgagatcgc tcaccaccac gctccccggc
     3781 tccgccaccc ggccgagatc gttgcgccca aggtcgacca gcatgatgtg ttccgcgcgt
     3841 tccttgtcgt cgccggccag ggccgccgcg ttccgcgcat cctcttcctc gtcggcgccg
     3901 cgcgggcagg tgccggcgat cggccgatac tcgaggcgcc cgtcgtgcag gcgcaccagg
     3961 ggctcgggcg aggaacccac caccggtgcc cgaccgtagt tcaggaagta catgtagggc
     4021 gatgggttca cggtccgcaa cgagcggtag aggtcgaacg gtcgggccgt ggtcggtact
     4081 tcgaagcgtt gcgagaggac cacctggaag atgtcaccgg cgcggatatg ctcgagcgcg
     4141 gtcgtcacca tcgcctcgaa ttcctcgcgc cgcatgttgg agttgggcgc ggcgggtgcc
     4201 tccggcgcgc gcgccgccgg agtggccggc agctcggtcg tcagcatcga ctcgatccgg
     4261 tcgagccggg ccacggcatc ggcccaggcc gcttcgaaat gctccggcgt gacggcatcc
     4321 ccgacatggg cgttggcgat caggaccagg cgtccgcgcg cgtgatcgaa ggcaaggacg
     4381 gtgtcgaaca ggcggagctc gacgtcggcc agcccccggt cgtcccgggc ccgctcggga
     4441 agctcctcga tccagcggac ggtgtcgtag ccgaaccagc cgacggcgcc acccgtgaac
     4501 cggggaagtc cgccgacccg ggcggcccga tacggcgcca ggagctcgcg cagcgcggcg
     4561 cggggatccc gggcctcgaa gatgcgggtc gtgccggcac gatggtccac cacctggacg
     4621 taccggccgt ggatcgtcac ggccaggaag ggatcacgcc cgaggaagga gtagcgggcc
     4681 agccgctccc ctccctccac gctctcgagg agaaacgatt cggtgttcgg gccgtcgatg
     4741 cgaagaaaag ccgccacggg tgtcatgaga tcggcggcga tctcccggtg caccggcacc
     4801 aggttgccgt tggccgagcg ctcgcggagc ccttccggtc ccggattttc tagcacgccg
     4861 gctcacgcgt cacgaggttc gttccgaggg cgtcgcggaa gagatcggcg ttgaccagcc
     4921 ggccggtcca gagcgcgaac tgcgccgcac cctgccgcgc aaggaagtca tgccctccca
     4981 ccacccggtg acccgccgcg tgcgcggccg actcgagcgg cgtcatccca tcacgataag
     5041 ccaggtcgag gaggcgcccc ccggcgccga gccagcccgg gagccgaacc gtttctcccg
     5101 ccgcgcccag cggcgtgcac tggatgaaga tcgttccggt caccgcatcg agtgcgtcgg
     5161 gcttcccggc aaccgtgacg cccgcgaact tgtcggccag gaggcgcgcc cgcttcgtgt
     5221 cgcgattcag gatcgtgacc ggcacctgtc gttcggcaag cgccgccacc acggcgcgcg
     5281 ccgcgccccc cgcgcccagc accaccgccc ggtcgtccgg cccggccgac cattcgcgca
     5341 ggatggtccg catcgcgagg aggtcggtgt tccatccgac cagtcgatca tggtcgcgca
     5401 ccaccgtgtt cacggcaccg gcggatcgcg ccgtggcatc gaggccgtcg aggagcggca
     5461 cgatcgtttc cttgtgcggt cgggtgaccg cgaggccacg gatgtcgttg tgccgcacgt
     5521 agtcgaccac ctcggcgatt gacggcgatt ccagggggag catcaggtaa cgctccccga
     5581 gcacccggat cacgccgttc tgcatccggg gcgagaggga gtgaccgagc ggatggccgg
     5641 ccaggccaag cagcttgacc gactgcccct gtgcggggag ccggtacatc gtgatcagct
     5701 ccgaaacatg cggaatcgat ggcgggaggc cggtctcgtc gtcgggaacg aggtaggtca
     5761 gccacgcgcc ggccaggtaa ccgagcacgc ggccggccag ggcggcggct cccgccgaga
     5821 agcaggtgac acgacgaccc gaccggacga ggtggcgcgt caggtcgacc agccgcatcg
     5881 attcggtgaa gctggccggt gtcgccacca gtttcacgcc ggccgcctcg acgcgcgtca
     5941 tggccaggag ttcgtcctcg atggcgtgac cggcatccgc cccagcgtga tgcgagacga
     6001 gcagtcgatc ggccggccag cgggcggcca atccgtgatc gcgacggatg cggctccact
     6061 cgatgtcgac caggcgcgca ccggcgccga gagccgcgga gagaagggcc cagcggtcgg
     6121 cgtcgggccc gtcatgaaag cccgactcat cgatcgggcg gcaggtggcg atgaccggca
     6181 gggccgcggc gcgtaccagg tcggcgacga cgggcgccgt ccgttcctcc ggcgcgagac
     6241 ggtcgatgcg cagttcgagc atgtcgatgc cgtccggcgg cgccgcgagc gcccgacgcg
     6301 ccttctcccg atcggcggcc gccacgacga ggacgagatt ccgttcggac ttcatgcgac
     6361 ccccttcgtt ccgtgaagct tgccggggcg atacccctcg ggatcgatct ccacgtcgtc
     6421 gaagccggcg cccaggagcc cggcggtcac ccgcccgcgc cacggctcgg acaggaggcg
     6481 cgacacgtcg accgggtcta cttccacgcg cgcgcaccga ccaagatcac gcacgcggaa
     6541 ctgccggaaa ccggcctcgc gcaggacgac ctccgccctc tcaacgcgcg ccaggcgctc
     6601 ggtcgtcacc ggcgtcccgt gcgggatgcg cgacgcgagg cacgcgaagg ccggcttgtc
     6661 ccagatggaa agcccggcct cgcgcgcgag cagacgaatg tcggccttgt cgagatcggc
     6721 ctcgaggagc ggcgcgcgga tcgcgcgctc acgcgccgcc tgcatcccgg gacggaagtc
     6781 gccgaggtcg tcgcgattgg ccccgtaggc cacgtgggcc agtccgagcc gctcggccac
     6841 cgggccggcc acgtcgaaga gctcggtctt gcagaagtag catcgattcg ggttgttcgc
     6901 cacgtactcc gggttcatgt gctcgaaggc ctcgacgacc tcgagccgcg cgccgatttc
     6961 ggcggccacc tggagcgccg cctcgtgctc cgcggccggc agcgacggcg acaccgcgag
     7021 caatccggcg gccccgctgc cgagaacccg atgcgcggcc aggagcaggt acgccgagtc
     7081 gacgcccccc gagtaagcca ccagtacgga gcccatgtcg gcaaggattg cctcgagccg
     7141 ggccgcgcgc ccttcgagcc cctcacgaac cgcaatcacg ggaacgacgc ggtcactcgc
     7201 catggcggtc cccctcgaag agaccgggca caccgagata acgttccccg tggctggcca
     7261 ggagcgtcac gatccgacga ccgttcatct ccggtcgttc ggccagccgc atggaggcgg
     7321 tcacggccgc gccgctcgag atgccgacca gcaatccctc ctcgcgcgcc agccggcgcg
     7381 tcatccgcat ggcgtcggcg ccggacaccg tcatcacctc atcgaggatc gcgcgattca
     7441 acaccggcgg aacgaaaccc gccccgattc cctggatgtc gtgcggtgct ccctcgccgc
     7501 ccgagagcac cggcgattcg gccggctcga cgccgacgat ccgtacgcgc ggcagccgcg
     7561 ccttcagcac ctcgcccacg ccggtaatcg tgccgccggt gccgacgccg gccacgaagg
     7621 catcgagcct gccctcggtg tcggaccata tttcctcggc cgtcgtgcgc cggtgcacgt
     7681 cgggattggc ggggttttcg aactgcatcg gcacgaaggc gcccggcgtg cgctcggcca
     7741 gcgccatcgc ctcctccacc gcacccttca tcccgagcgc gcccggcgtg aggatgacgc
     7801 gcgcaccgag cgcggcgagc agcttgaccc gtccttcgct catcgtgtcg ggcatcgcga
     7861 ggacgagctt gtagccgcgc gccgccgaca cgaacgcgag tgccacgccg gtgttgccgc
     7921 tggtcggctc gatgatggtg ctcccgggcg caagccgccc gaccttctcg gcggcctgga
     7981 tcatggccca gccggtccgg tccttcacgc tcgacatcgg gttcatcgac tcgagcttca
     8041 cgatgatttc ggccggcagg ccggccgtca cccggttgag gcggacgagc ggcgtcctgc
     8101 cgatcaggtc ggtgaggttg tcgtggatcg gtgccatcgc gtcgatgcgt ctccgtcgtg
     8161 ccggcctctt cggggccgct gtccgtcaga tctggtagat cagcgactgg tcgtgccggc
     8221 gcttcttccg gcagaggtcg tcgagtgtcg ttccttcgag gacgttttcc atcgcctgcg
     8281 aaagctcgcc ccagaattgc gcgaggatcc gggacgactc tccctgggct cccgcttcct
     8341 tcccgaaccc cgtcacgtcg agccgccctt cgagcgcttt caggatctcg gccaccgtca
     8401 cctcgtccgg ccggcgcgcc agtcgatgcc cgccgttcgg cccccgcgtg ctcacgacca
     8461 gcccctgtcg gcgcagcgcc gggaggatct gtttcaggta ctgctcggga atccgctgcc
     8521 ggcgcgagat gtcatggttc ggcaccggcc cgccccccgc gtgcagcgcc aggtcgagca
     8581 ttgcccgcag tccatagtcg atccgggatg tcagcctcat gccgatgtcc tcccgccgcc
     8641 gaacgattgt tgagattgtt gagtagttta ctcacggatg gtcgggacgt ccatccgaac
     8701 cgtcgatcag tcgccggtgc gccgagattc gagcgactgc cccccctcgc ggccgagcgc
     8761 cgccaggtgg aggatggcct cgagcacgcc gccggcggcg gcccgggcca acgggtcgat
     8821 ggccgagacg cgttcggcgt cggcgcccgg gtcgatctcg gccacccgca tccccggccc
     8881 gacgaagagg ccgcctgcca ggagcccgat gacgagaccc gcgatcggtg cgcgaacctc
     8941 gcgtcctccg acggtgccca gcacagcatc gacttccacg gagatgccga tcgatgccgt
     9001 ggattcgaaa cgcccgggga tcggcgcgcg gacgagcccg cgatcgacag cgccggccgc
     9061 ggccagcgaa aggggcgcct cgcggagcgg cccctcggtc gtgaagcggc cgagatccac
     9121 gttgggcctt gtctccacca ccgcgtgcgc gtcagcgccg gccgaaaacc ccggccccat
     9181 ggccaccacg atcggcgcgt cctcgcgccg cgtgccgaca ttccattcaa ggccgatggc
     9241 atcgaccagg acccacggtg ccagcccctc gcggatcgtg gccgccggat ccacgaggac
     9301 cggcacgccc ccctcgtcga gcacctggcg gattccctcg atgccggagg cgcgtcggcc
     9361 acggacccct tcgaccgaca cttcggaatc ctcgatcgcc gcgccgaagg cggcgcgccg
     9421 gtggatcaac gtggggcggg ccgcctccgc gatcacgacc ggaaagccgc agagccggag
     9481 tcggtgcgcg gtggccgtgc cgaggcgacc gccgccgcgg accaggacga tatcgctcat
     9541 cgcatgcgcc ccggcgaggc aagtccgttc cggaccgcca cgagctc
//
LOCUS       DASVOD010000031         5896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_627739, whole genome shotgun sequence.
ACCESSION   DASVOD010000031 DASVOD010000000
VERSION     DASVOD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627739"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VF720_00620"
     CDS             <1..1602
                     /locus_tag="VF720_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877876.1"
                     /translation="AISPDGRTLVFTASDSVGTTRLWIRPLDGLMARVLPGTDRAIQP
                     FWAPDGRSIGFFADTKLKTIRIETGRVETLGDAPDPRGGSWGSNGSIVFAPIAMGSLY
                     SVPSEGGAVTEVARPDTTRGETALRYPEFMPDGRKFLFVALPVRDGNHQVYLGELGSS
                     NRTALLQAGASPAYANDGWLATLKEDRLVAQKFDGRQGKPIGTPVVLGDAPILGGNSG
                     TRAASASRNGILAYSAARRSDTQLAWLDRSGRVESRFSLPAGRWESVAISPDGSRAAV
                     LRRDGSDSYGLWIVDLTTGQATSSGRVAELGQSLVWSPDGTRIMVSASPDGPTDLYMQ
                     AVDSGEMHLLYASNVLFKNCYGWAPDGQFIVFESPSQETGWDVWKLSVEPGSQPVPLV
                     RTQFDEGGGWFSPDGRWLVYYSDESGLYELFVQPLIGTGRRQSIPGTRTAGPQGPSPC
                     WWADDGREILMRLPDGTMRVAEVEPGPDFQCRRARILFKFGDDVAGICPTPDHRRFLA
                     TIDVEEAAAPAIVVDLNWPSVLKKK"
     gene            complement(1645..2664)
                     /locus_tag="VF720_00625"
     CDS             complement(1645..2664)
                     /locus_tag="VF720_00625"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7877877.1"
                     /translation="MAGFDGLDVMLERVVRETDFSGVIEIREGDVVRFARAWGHAERE
                     RGIPITLDTRFGIASGTKFLTALAIGHLIAEGRIELDTRLSEVLALDLPDYPPDITIR
                     HLLTHTSGIPDYYDEDKVESFDTFALPIPFDRLLGPRDYLPFFPREPMKFAPGSAFSY
                     SNGGYILLGVIVEELSGMGYQTYVARTILEPLGMSRSGFFAMDQLPEGTAIGYVAQPE
                     GGWRTNFGILPIIGASDGGMFLTPGDLNRLWTAFWSGAILPASLVEIYATPHVRAAAD
                     SARWYGHGLWMRERAPGEWEHWLEGCDAGVSFQSIALRSTHQRITVMSNTTDGAWPIL
                     RAMGR"
     gene            complement(2673..3617)
                     /locus_tag="VF720_00630"
     CDS             complement(2673..3617)
                     /locus_tag="VF720_00630"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7877878.1"
                     /translation="MKLAWRNLQIGLFFLGGLAILTIALLIVGTNQNLFSPVYRLKGY
                     LPDTQTLAEGTSVTLSGIEIGVIEKISLDTWQGQNAVRFDIRLKKEFQSRITTSSHGV
                     VKSIGVLGDKFLEITMGKAGEPVLADGGIIPIEPAIDFEKLMKDVSGSLVSSLHRVEG
                     ILDEVEAGHGTLGMLVRDSTLVVRLNDALGSLDQTMTAMRSGKGTMGRMINDPAIYDD
                     LAETTKRLKSITDKIDRGEGSLGKLIGDSTLYVSAAHAATGLDTTMARINAGEGTVGK
                     ALNDDAAYDEMTKTIQEVRNLLLELQKDPRKFLKFSVF"
     gene            complement(3614..4345)
                     /locus_tag="VF720_00635"
     CDS             complement(3614..4345)
                     /locus_tag="VF720_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7877879.1"
                     /translation="MSRPAVIELRNVTFRFDTRMILDDVSIAIEEGTTRCILGASGAG
                     KSTILRLILGLLTPEKGEVWVLGKDVTKLKGKELQELRHSIGMVFQSGALFDSLAVWE
                     NIGYCPLETHQEKREPVRQKVRELLAMVGLGEVLDMMPSELSGGMKKRVAIARSFICG
                     PKVMLYDEPTTGLDPIVSDTINELINRLRNQNKVTSIVVTHIMRDAYYVADSMTLLRD
                     GKVVFDGTPAEIKVSPDPYIREFIS"
     gene            complement(4342..5112)
                     /locus_tag="VF720_00640"
     CDS             complement(4342..5112)
                     /locus_tag="VF720_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7877880.1"
                     /translation="MADLTYPVKTFVFAAQEFWLLLWNSFLGFPAAFKYRKETVRQIY
                     AIGVTATPLILLAALLIGLVLALEWGNKLEPFGAKILMGRIMAVGTIREIGPVITGLM
                     FAGRTGAMIAAELGSMKVTEQIAALTALGTDPVRRLVVPRQVASVVTILPLTVIADAV
                     AILGGYVVAVAWLHTPGKFYWTSALNSLAWEDLAIGVLKPFFFGWMISTVGCYYGMTT
                     EGGASAVGTATTKAVMYASLGTLAADFVLSKLILSFFR"
     gene            complement(5203..5754)
                     /locus_tag="VF720_00645"
     CDS             complement(5203..5754)
                     /locus_tag="VF720_00645"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7877881.1"
                     /translation="MSTSDALAERYASAPDWPAYLASTVKNHDLWSGIDRHVAIDADQ
                     VDLARKAAERGGVRHVVVLSEDWCGDAVNIVPWVGRLVGMLPGVDLRIFGRDANPDLM
                     DAHLTGSSRSIPVIILYDSQFRELGWWGPRPTELQALALGPWCSLPKDERYREIRTWY
                     ARDKGSTILAEIIALMSRASSPA"
BASE COUNT         1024 a         1856 c         1950 g         1066 t
ORIGIN      
        1 gccatttcgc ccgacggacg caccctggtc ttcacggcat ccgactcggt gggcaccacg
       61 cggctctgga ttcggccact cgatggcctc atggcccgtg tcctccccgg aacggatcgg
      121 gccatacagc cgttctgggc gccggacggg cgctcgatcg gattcttcgc cgacaccaag
      181 ctcaagacca tccggatcga gacaggccgt gtcgaaactc tgggcgacgc gccggacccg
      241 cgcgggggct cgtggggaag caacggctcg atcgtcttcg ccccgatcgc gatgggctca
      301 ctgtacagcg taccgtcgga gggaggagcc gtcaccgagg tggcccgccc cgacacgacg
      361 cgcggcgaga cggccctgcg gtatcccgag ttcatgccgg acggccggaa atttctgttc
      421 gttgccctcc ccgtccgcga tggcaatcac caggtctacc tgggagaact cggatcgagc
      481 aaccgcacgg ccctgctgca ggccggggcc tcgccggctt atgccaatga cggctggctc
      541 gcaacgctca aggaggatcg gctcgttgcc cagaaattcg acgggcggca gggcaagccg
      601 atcggtaccc cggtcgtgct tggcgacgcg cccattctcg gcggcaattc ggggaccagg
      661 gcggcgtcgg cgtcgcggaa cgggattctg gcatattcgg cggcccgtcg gtccgatacg
      721 caactggcct ggctcgaccg ctccggtcgt gtcgagagcc ggttttcgtt gcccgcagga
      781 cgatgggaat ccgtggcaat ttccccggac ggcagccggg cggccgttct gcgccgggac
      841 ggatcggata gctacggcct gtggatcgtg gacctgacca ccggccaggc aacttcgtcc
      901 ggcagggtcg ccgagctcgg tcaatcactg gtctggtctc cggacggaac acgaatcatg
      961 gtcagtgcat cccccgacgg ccctacggat ctctacatgc aggctgttga ctccggcgaa
     1021 atgcacctgc tttacgcctc gaatgtgctg ttcaagaact gctatggctg ggcacccgac
     1081 ggtcagttca tcgttttcga gtcgccaagc caggagacgg gctgggacgt ctggaagctc
     1141 tccgtcgaac cgggcagtca gccggtgccg ctggtacgga cccagttcga cgaaggaggg
     1201 ggctggttct cgccggacgg tcgctggctg gtctattact cggacgagtc cggcctttac
     1261 gaactcttcg tccagccgct catcggaacg gggaggcgac aatcgattcc ggggacgcgg
     1321 acggcggggc ctcaggggcc gagcccgtgc tggtgggcgg acgacggtcg cgagatcctg
     1381 atgcgcctcc ccgacggaac catgcgtgtt gccgaagtgg agcccggtcc cgacttccag
     1441 tgccgtcggg cacgtatcct gttcaagttc ggcgacgacg tcgccggcat ctgcccgaca
     1501 ccggatcatc gccgcttcct cgccaccatc gatgtcgaag aggccgcggc gcccgcgatc
     1561 gtcgtggatc tcaactggcc gtcggtattg aagaaaaagt gatgatgtca atcgtcggac
     1621 gcaacccgtg cgacgaaatc ccggctaccg acccatcgca cgcaggatcg gccatgcccc
     1681 gtccgtcgta ttggacatca cggtgatgcg ctggtgcgtg ctgcggagag cgatcgactg
     1741 gaacgacacc cccgcgtcgc acccctcgag ccagtgctcc cattctcccg gggcccgttc
     1801 gcgcatccac agtccatggc cataccacct ggcgctgtcg gccgccgccc gcacatgcgg
     1861 ggtggcatag atctccacga gcgaggcggg aaggatggcg ccgctccaga aggccgtcca
     1921 aagccggttc aggtcgccgg gcgtcaggaa catcccgccg tcggatgcac cgatgatcgg
     1981 caggatgccg aagttcgtgc gccatccccc ctcgggctgc gccacgtagc cgatcgccgt
     2041 tccctcgggt agctgatcca tcgcgaagaa tccggaccga ctcatgccga gcggctcgag
     2101 gatcgtacgg gcgacgtacg tctgatatcc cattccgctc agttcttcga caatgacgcc
     2161 caacaggatg tagccgccgt tggagtacga gaaggcgctg cccggcgcga acttcatcgg
     2221 ctcacggggg aagaatggga gatagtcgcg cggcccgagg agccggtcga atggaatcgg
     2281 gagggcaaac gtgtcgaagc tctcgacctt gtcctcatcg tagtaatccg gaatgcccga
     2341 cgtgtgggtc agaaggtgcc ggatcgtgat gtcgggcggg tagtcgggga ggtcgagcgc
     2401 gagaacttcg gacagacgtg tgtcgagctc gatccgccct tcggcaatca aatgtccgat
     2461 ggccagtgcc gtcaggaact tcgttccgga cgcaatgccg aagcgggtat ccagcgttat
     2521 cggaatgcct cgctcgcgct cggcatgacc ccaggcgcgg gcgaaccgga cgacgtcacc
     2581 ctcgcgtatc tcgatgacac cggagaagtc tgtttcccgg accacccgct cgagcatgac
     2641 atcgagccca tcgaagcccg ccatgcaacg gctcagaaca cgctgaactt gaggaacttc
     2701 ctgggatcct tctgcagctc caacaggagg ttccggactt cctggatcgt cttcgtcatc
     2761 tcgtcgtagg cggcatcgtc attgagcgcc ttgcccacgg tgccctctcc cgcgttgatc
     2821 cgggccatcg tcgtatccag cccggtcgcc gcgtgggcgg cgctgacata cagcgtcgaa
     2881 tcgccgatca gcttgccgag ggatccttcg ccccggtcga tcttgtcggt gatcgactta
     2941 aggcgcttcg tcgtctcggc caggtcgtcg tagatggccg ggtcattgat catccgcccc
     3001 atcgttccct tgccggagcg catggccgtc atggtctggt caaggcttcc cagggcatcg
     3061 ttcaggcgca cgacgagcgt cgaatcgcgt accagcatgc ccagtgtgcc gtggccggcc
     3121 tccacttcat cgaggatccc ctcgacgcgg tggagcgaac tgaccagcga accggaaacg
     3181 tccttcatca gcttttcgaa gtcgatggcc ggttcgatgg ggatgatgcc gccgtcggcc
     3241 aggacgggct cgccagcctt ccccatggtg atctcgagga atttgtcgcc gaggacgccg
     3301 atggacttga ccacaccgtg gctggaggtg gtgatgcggg actggaactc cttcttgagg
     3361 cggatgtcga aacgcaccgc attctgcccc tgccaggtgt cgaggctgat cttctcgatg
     3421 acgccgatct cgatgccgga aagggtgacg ctcgttcctt cggccagcgt ctgcgtgtcg
     3481 gggaggtacc ccttcagccg gtacacgggg ctgaagaggt tctggttggt gccgacgatc
     3541 aggagcgcaa tggtgaggat ggcgaggccg cccaggaaga agaggccgat ctggaggttg
     3601 cgccaggcga gtttcacgaa atgaactccc ggatgtaggg atcgggtgac accttgatct
     3661 cggccggcgt gccgtcgaag accaccttgc catcacgcag cagggtcatg gagtcggcca
     3721 cgtagtaggc gtcacgcatg atgtgggtca ccacgatcga cgtcaccttg ttctggttcc
     3781 ggaggcgatt gatcagttcg ttgatggtgt ccgaaacgat ggggtcgagg ccggtggtgg
     3841 gctcgtcata gagcatcacc ttcgggccgc agatgaacga tcgggcaatg gccacccgct
     3901 tcttcattcc gccggagagt tcggacggca tcatgtcgag cacctcgccc agccccacca
     3961 tcgccagcag ctcgcgcacc ttctgtcgga ccggttcgcg cttctcctgg tgcgtctcga
     4021 ggggacagta gccgatgttt tcccataccg cgaggctgtc gaacagggcg ccgctctgga
     4081 acaccatccc gatggagtgg cggagttcct gcagctcctt tcccttgagc ttggtgacat
     4141 ccttccccag cacccagacc tctccttttt cgggcgtaag gaggccgagg atcagccgga
     4201 ggatcgtgct cttgccggcg ccggaggcgc ccaggatgca ccgggtggtc ccttcttcga
     4261 tcgcgatcga cacgtcgtcc aggatcatgc gcgtgtcgaa tcggaacgtg acgttccgaa
     4321 gctcgatgac ggccggtcgg ctcatcggaa aaacgagagg atgagcttgg acagcacgaa
     4381 gtcggcggcc agcgtgccga ggctggcata catcacggcc ttggtggtgg cggtgccgac
     4441 ggccgaggcg cctccttcgg tcgtcatgcc gtagtagcac ccgacagtgg agatcatcca
     4501 gccgaagaag aagggcttga ggacgccgat ggccaggtcc tcccaggcca ggctgttgag
     4561 cgcactcgtc cagtagaact tgccgggcgt gtggagccag gccacggcca ccacgtatcc
     4621 tccgaggatg gccaccgcgt cggcgatgac ggtgagcggc aggatggtga cgacgctggc
     4681 cacctggcgc ggcacgacga ggcggcggac cgggtcggtg ccgagcgccg tcagcgcggc
     4741 gatctgctcg gtcaccttca tcgagcccag ctcggcggcg atcatggcgc cggtgcggcc
     4801 ggcgaacatg aggccggtga tgaccgggcc gatctcgcgg atggtgccga cggccatgat
     4861 gcgccccatg aggatcttgg cgccgaacgg ctcgagcttg ttgccccact cgagggccag
     4921 cacgagcccg atgagcaggg ccgccagaag gatgagtggc gtggcggtga cgccgatggc
     4981 gtagatctgg cggaccgttt ccttgcggta cttgaaggcc gccggaaagc cgaggaacga
     5041 gttccagagg aggagccaga actcctgggc ggcaaagacg aatgtcttga ccgggtatgt
     5101 caggtccgcc acgggcgcgc tccggagttt cgggattggc gggttctgga agtcatgccg
     5161 ccggacaaag aggctcgaat cctaatggga tgcccccgag agtcaagcgg gggatgaggc
     5221 ccgcgacatg agggcgatga tctcggccag tatcgtgctc cccttgtccc gggcatacca
     5281 ggtgcggatc tcgcggtagc gttcgtcttt cgggagggaa caccaggggc ccagggccag
     5341 cgcctgcagt tcggtgggac gcgggcccca ccagccaagc tccctgaact ggctgtcata
     5401 caggatgatg accgggatcg accgactcga cccggtcagg tgggcgtcca tcaggtccgg
     5461 gttggcatcg cggccgaaaa tcctgagatc gactcccggc aacatgccca ccagccggcc
     5521 cacccagggc acgatgttca cggcgtcacc gcaccagtcc tcggagagga cgaccacgtg
     5581 gcgcacgccc cctcgttccg ccgcctttcg ggccaggtcc acctggtcgg catcgatggc
     5641 gacgtgccga tcgatgccgc tccagaggtc gtggttcttg accgtggagg ccaggtaggc
     5701 cggccaatcc ggggcggatg cgtaccgttc ggccagggcg tcggaagtgg acatgacggg
     5761 ttggatgatc gcaggtttct ttcgatccgt ggtcacttcc gtctcgcgga gttttcgcct
     5821 tcgtgaacgc ggaaattgac acctaaagtc atccggtgct aaggtttacg cccttccgtt
     5881 ctccaggtga gacccg
//
LOCUS       DASVOD010000032         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_523468, whole genome shotgun sequence.
ACCESSION   DASVOD010000032 DASVOD010000000
VERSION     DASVOD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523468"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1949)
                     /locus_tag="VF720_00650"
     CDS             complement(<1..1949)
                     /locus_tag="VF720_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7877882.1"
                     /translation="MTTPRTSHRNARPHAGSNRNHVRRQALTGRPGGFTLVEVMMALV
                     LFGAVMGAIYTVFTRTQKAQSFGRELSETQQNARAALDAMAADLRAAGYGIDPTSAVA
                     IEVASDYRVTFRRDVNRNGTIDLGEMITYFVDPTLTDAMVSDTENPRDCVLRKVVSSA
                     GNATAEPAAGTGDIVAFGITQIASGNGTTWNAPLFTYRDEAGTDLMGSTNDPDDVVWG
                     HSLQDSVLGKPAGGTNDLLIESIAINVIAETPRPNPQTGQYRKTSITARVLPRNLPLE
                     YASAIQSGVTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGLPPYEPPIHISTVNVLT
                     LTLADVDEVDPNEGSATTTDNQHDLDIVLGTKAGASNNLDVWWNGQPGKYIGGARNYN
                     SVPNYRGSATYDVYGLAVGYIDGNTTRDCVTAYRISDNTGGFYVWLNQGPAPSSSYMD
                     GRLGVGSTSPTTYNASYRHSQSGKGQSMALADLDNDGDKDVVMGTRTNANAGKVEVWF
                     NNGFGIFTANSSTDVYTAAGEVQSVALMDVNADGRIDIITGCKTSNNNNNGSFDIFTM
                     NTTPPGKFTKLSTTIAGGNIRSVAAGQMNNDSYPDLVFGERTGSNTGRVELWTNNGSG
                     VFSMADQVAADGPVTCVALGLIDQDL"
     gene            complement(1973..2371)
                     /locus_tag="VF720_00655"
     CDS             complement(1973..2371)
                     /locus_tag="VF720_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877883.1"
                     /translation="MIRRLKRRTERRTTASAGFSLIEVMFAIAFLGFGLMAVAQMIPL
                     ATRQMVSSKQLTDGVEAGQSLFEELKMDDYGGSTLTAGTHTRTVGQYELEWLVTNNEP
                     VAGSKRVELTVSWNVSGQTQSATMATYITR"
     gene            complement(2368..2874)
                     /locus_tag="VF720_00660"
     CDS             complement(2368..2874)
                     /locus_tag="VF720_00660"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HEX7877884.1"
                     /translation="MNPFRRSNPSAPRSGERGFSLVELMVSIALMSIMFLVGLPAMNR
                     YLKTNRLLAASANLAGELRLARQRAVSEENNVVFSWNLTDDKYRWLDDDDNDGSADTG
                     EYVSEWRQLPDGIDLDAGATPLAGTSVTFLSSGSASQGGQLTVTNEEGLERTIQLVQV
                     SGLVKVLS"
     gene            complement(3226..>3821)
                     /locus_tag="VF720_00665"
     CDS             complement(3226..>3821)
                     /locus_tag="VF720_00665"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HEX7877885.1"
                     /translation="LRGRCRDCKAPISSRYPLVEAASALLVVACFARWGVTPTAAIHA
                     AFCLSMLVITLIDLDHRIIPDSITLPGTILALALVNWTEPSWLDAVIGAVAGFAVLYG
                     VGEAYHRSTGIEGMGGGDIKMAAYMGAMLGWKGVLLTIFLGALLGSVAGVTTMMVGKG
                     NRRTALPFGTFLAPAAVITLFFGQSIIDGYLRLIFRN"
BASE COUNT          601 a         1186 c         1327 g          707 t
ORIGIN      
        1 aggtcctggt cgatcaggcc gagagccacg caggtgacgg gcccgtcggc ggcgacctgg
       61 tccgccatgg agaagacccc cgagccgttg ttggtccaga gttcgacccg gccggtgttg
      121 ctgccggttc gctcaccgaa caccaggtcg ggatagctgt cgttgttcat ctgtccggcg
      181 gccaccgagc ggatgttgcc gccggcgatc gtcgtggaga gcttggtgaa cttgccgggt
      241 ggcgtggtat tcatcgtgaa gatgtcgaag gatccattgt tgttgttgtt cgatgtcttg
      301 cacccggtga tgatgtcgat ccgcccgtcc gcattcacgt ccatcagtgc cacgctctgc
      361 acctcgcccg ccgccgtgta gacgtcggta ctcgagttgg cggtgaagat cccgaacccg
      421 ttgttgaacc acacctccac cttgccggca ttcgcgttgg tcctggtgcc catgaccacg
      481 tccttgtcgc cgtcgttgtc gaggtcggcc agcgccatgc tctgtccctt gcccgactgg
      541 gagtggcggt aggacgcgtt gtaggtggtg gggctcgtcg agcccacgcc gaggcgtcca
      601 tccatgtacg agctgctggg ggccgggccc tggttcagcc acacgtagaa gccgccggtg
      661 ttgtcggaaa tccggtaggc ggtgacgcag tcgcgggtgg tattgccgtc gatgtatccg
      721 accgccaggc cgtacacgtc gtaagtggcg ctgccgcgat aattcgggac ggaattgtag
      781 ttccgggcgc cgccgatgta cttcccgggc tgcccgttcc accagacgtc caggttgttc
      841 gatgcgccgg ccttcgtgcc gaggacgatg tcgaggtcat gctggttgtc ggtcgtggtg
      901 gccgaaccct cgttcgggtc cacctcgtcg acatcggcca gcgtaagcgt gaggacgttg
      961 accgtgctga tgtggatggg cggttcgtac ggaggaagcc cggtgcccgt gccggtaccc
     1021 gttccagtgc cggttccagt gccggtaccg gtcccggtgc cggttccggt gccagttcca
     1081 gttcccgtgc cggtcacgcc ggactgaatc gcgctggcgt actcgagcgg caggttacgc
     1141 ggaagcacgc gggccgtgat gctggtcttg cgatactggc cggtctgcgg gttcggacgg
     1201 ggcgtttcgg cgatcacgtt gatggcaatc gactcgatca gcaggtcgtt cgtgccgccg
     1261 gccggcttgc cgaggaccga atcctgcaga gaatggcccc acacgacgtc gtcgggatca
     1321 ttggtggatc ccatgaggtc cgttccggcc tcgtcgcgat aggtgaagag cggcgcgttc
     1381 caggtcgtcc cgttccccga ggcgatctgg gtgatgccga atgccacgat gtcaccggtg
     1441 cccgcagccg gttccgcggt ggcgttgcct gccgaggaga cgaccttgcg caggacgcag
     1501 tcacgcgggt tctcggtgtc cgacaccatc gcatcagtca gggtaggatc gacgaagtac
     1561 gtgatcatct cccccaggtc gatcgtcccg ttgcgattca cgtcgcggcg gaacgtgacg
     1621 cggtagtccg atgccacctc gatggccacc gccgacgtcg ggtcgatccc gtagccggcg
     1681 gcccggaggt cggcggccat ggcgtcgagt gccgcgcggg cattctgctg ggtttccgag
     1741 agctcgcggc cgaagctctg ggccttctgc gtgcgcgtga agacggtgta gatggctccc
     1801 atgacggcgc cgaagagaac cagggccatc atgacctcga ccagcgtgaa tccgccgggc
     1861 cggcccgtca gcgcctgccg tcgcacgtga ttccggttgc tgccggcgtg cgggcgcgcg
     1921 ttccgatgag aggtgcgggg ggtcgtcatg acaggctctc cttccatcct cgtcaccgtg
     1981 tgatgtaggt ggccatggtg gccgactgtg tctggccgga aacgttccac gacacggtga
     2041 gctcgacgcg cttgctgccg gccaccggtt cgttgttggt cacgagccac tcgagttcgt
     2101 actgaccgac cgtacgggtg tgggtgccgg cagtcagagt cgaaccgccg tagtcgtcca
     2161 tcttgagctc ttcgaagaga ctctggcccg cctccacacc gtcggtcagc tgcttggacg
     2221 acaccatctg ccgcgtggcc agcgggatca tctgggccac ggccatcagg ccgaagccga
     2281 gaaaggcgat ggcgaacatg acctcgatga gactgaaccc ggcggaggcc gtcgtgcgcc
     2341 gttcggttcg gcgcttgagt cggcggatca cgagagcacc ttgacgaggc cggacacctg
     2401 cacgagctgg atcgtccgtt cgagcccttc ctcgttcgtc acggtcaact gacctccctg
     2461 cgatgcgctg ccgcttgaga ggaacgtcac cgacgttccg gccaacggcg tggcgccggc
     2521 atccaggtcg atcccatcgg ggagctgccg ccattcgctc acgtactccc cggtgtcggc
     2581 ggagccatcg ttgtcgtcgt cgtcgagcca ccggtacttg tcgtccgtga ggttccagct
     2641 gaagaccacg ttgttctcct cggaaacggc acgctggcga gccagccgaa gctcgcccgc
     2701 caggttggcg ctggccgcga gaagccggtt cgtcttgagg tagcggttca tggccggcag
     2761 tccgaccagg aacatgatcg acatgagtgc gatcgatacc atgagctcga caagcgagaa
     2821 gccgcgctca ccggacctgg gcgcggacgg gttggaccga cggaacgggt tcaagtgccg
     2881 gactcctctc catgcggtgg attgcgcccc cggggggcga tctcgaccgg acaaacgatg
     2941 cggtcacccg ccggacagac cggcagggtc ccgagtggat ggctgcaaga gcgggaccat
     3001 tccgctccgg tgggtcctga tcgtcgggag gtcatcggca atcgggcgcg ggaactgcag
     3061 ttgtcgtttg gcgggtgatt tcgcgcaggg cgcgcagtcg gctcatcatc ggcccgacga
     3121 tgtggcggtc tgcacgccgg gtgcgtgcag ggcgcgatca ggtgcggcag gtcgatccgg
     3181 aagggcggcc ttgggacgat tcgttcggag acagagtccc gaaggtcagt tcctgaagat
     3241 caggcggagg tagccgtcga tgatcgactg gccgaagaag agcgtgatca cggccgccgg
     3301 ggcaagaaag gtcccgaacg ggagcgcggt gcggcggttt cctttcccga ccatcattgt
     3361 ggtgacgccg gccacggagc cgagcagcgc gcccaggaag atcgtgagca acacgccttt
     3421 ccacccgagc atggcaccca tgtaggccgc cattttgatg tcacccccgc ccattccctc
     3481 aatgccggtc gatcggtggt aggcctcccc caccccgtag aggacggcga agccggccac
     3541 cgcgccgatg acagcgtcga gccagctcgg ctccgtccag ttgacgagcg cgagcgccag
     3601 gatggtgccc ggcagcgtga tgctgtcggg gatgatgcgg tgatcgaggt cgatgagcgt
     3661 gatcacgagc atgctcaggc agaaagcggc atggatagcc gccgtgggcg tgactcccca
     3721 gcgggcgaag caggccacga ccaggagagc gctcgccgcc tcgacgagcg ggtagcggga
     3781 cgagatgggc gccttgcagt cgcggcagcg gccgcgcagc a
//
LOCUS       DASVOD010000033         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_210424, whole genome shotgun sequence.
ACCESSION   DASVOD010000033 DASVOD010000000
VERSION     DASVOD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210424"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VF720_00670"
     CDS             complement(<1..1187)
                     /locus_tag="VF720_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX7877886.1"
                     /translation="MTRIRANMLAMAGTFLVQAFFVIVHVKLLTRWLTPAEFGLYSSV
                     FALGAVLGSLAELGFSVVLPRYGATFEATGRPAAYRRLVWTAVRLWAVSGAVLCGLVG
                     MLSFLAVSPAGTGAISPGLLVLGLASALTFSLRSFASAAFQGLRKMGPALLLEFVYMA
                     GLTLWFIVFRESLDPFRVFAGFLALGLVTGAAGLVAFFRVAPHGAADEVRESDRDVLR
                     SIAPYWIGAFFTTVVAISVENADRLVLATLVPLSAVGAWHVAGRISLFLRKILFIFQQ
                     VAGPEFAWKWERGARQQLVGDLVLFMKVDWLIGVLLAIGVALLARFGVLFASNDRFLD
                     AVPALTAMAGSMTVLCLAAPITTFLRAGGRIDITVTAEILWLVGSMVIGAVLLKPFGL
                     FGFG"
     gene            complement(1184..2776)
                     /locus_tag="VF720_00675"
     CDS             complement(1184..2776)
                     /locus_tag="VF720_00675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7877887.1"
                     /translation="MRTPKPWHLALLVGLGIILAAVSWRGFDPEVIPGYSDYWDYLQL
                     GRQIAEGRGFTSRFTYPIFYLASGTGDAAGGFPLLWRPPLYPLVVAGGLVLTGGAVWT
                     PIVIGIVAYLAAILATYFLALEFTGRRWALLSGLVVALSPAMLGLDEPGLATTLYAAL
                     LALAVRAVLHAQTRVRAIVAGLAFGLLTLLRGEALFVLPAMIWLLWAGERGDRERRIW
                     AFLAAAIAITLPWTVRNFLVTGHPWFGTSSLLYIDTASYPGWTSSRMTDIGQRSAFGW
                     AFGHLGEVGWKGLKNLYHFLVQALLLPLAALAPFVWTAMGRLTGVGRESAYCASVLIA
                     LGLTIALLAPLEYAARFLNPFIPLLTVVGVIILGRMREQIGEGEGARYTRRPITWVAA
                     AVVTLAALQFLGALRDARAMRAAWEAEFGAMKGTDWRAVTAALPPNRLFEADYPAYYA
                     WKTGRRFLWWRAGCDGPAPPSAGRFPNDPGAERAGLLMRNRGVDRPDPMGIEPTPAIV
                     PHATSAHEIGNVVWLLLRPCPT"
BASE COUNT          540 a         1004 c         1007 g          303 t
ORIGIN      
        1 ccgaagccga agagcccgaa gggcttgagc aggacggccc cgatcaccat cgaacccacc
       61 agccagagga tctcggccgt cacggtgata tcgatccgcc cgccggcccg gaggaaggtg
      121 gtgatgggcg ccgccaggca gagcacggtc atcgatccgg ccatggcggt gagcgccggc
      181 accgcgtcga ggaagcggtc gttgctggcg aacagcacgc cgaaccgcgc gaggagcgcc
      241 acgccgatgg ccagcagcac accgatcagc caatcgacct tcatgaagag gaccaggtcg
      301 cccaccagct gctggcgggc gccgcgctcc cacttccagg cgaactccgg tcccgccacc
      361 tgctggaaga tgaaaaggat cttgcgcagg aagaggctga tgcgtcccgc cacgtgccac
      421 gcccccacgg ccgaaagcgg caccagcgtg gccaggacca ggcggtcggc attctccacg
      481 gaaatggcca cgaccgtggt gaagaaagcg ccgatccagt atggggcgat cgagcggagg
      541 acgtcgcgat cgctctcccg cacttcgtcg gccgcgccgt gcggggccac gcggaagaag
      601 gccaccagcc cggccgcgcc ggtcacgagg ccgagggcca ggaagccggc gaacacccgg
      661 aacggatcga gcgactcgcg gaagacgatg aaccagagcg tgagccccgc catgtagacg
      721 aactccagga ggagcgccgg acccatcttt cgcagcccct ggaacgcggc cgacgcgaag
      781 gagcggagcg agaacgtgag cgccgacgcc agcccgagca cgagcagccc cggggagatg
      841 gcccccgtcc cggccggcga gacggccagg aaggagagca tccccaccag tccgcagagg
      901 accgcgccgc tcaccgccca gagccgcacc gccgtccaga ccagccgacg gtaggccgcg
      961 ggcctccccg tggcctcgaa cgtggcgccg taccgcggca gcaccaccga gaagccgagc
     1021 tccgccaggc tcccgagcac ggcgcccagc gcgaagaccg acgagtagag accgaactcg
     1081 gcgggggtca gccagcgcgt caggagcttg acgtgcacga tgacgaagaa cgcctggacg
     1141 aggaaggtgc cggccatggc cagcatgttg gcgcggatgc gggtcacgtc gggcagggcc
     1201 ggaggagcag ccagaccacg ttcccgatct cgtgcgcgga ggtggcatgc ggcacgatgg
     1261 ccggcgtcgg ctcgattccc atcgggtcgg ggcggtcgac gccccggttc ctcatgagga
     1321 gtcccgcccg ctcggcgccg ggatcgttcg ggaatcgccc ggcggatggg ggtgccggcc
     1381 cgtcgcagcc ggcccgccac cagaggaacc gccgccccgt cttccatgcg taataggccg
     1441 ggtaatcggc ctcgaatagc cgattcggcg gcaacgctgc ggtcaccgcg cgccagtcgg
     1501 tccctttcat cgccccgaac tccgcctccc aggccgcccg catcgcccgc gcatcgcgca
     1561 gtgcacccag gaactgcagc gccgccagcg taacgaccgc cgccgccacc caggtgatcg
     1621 gccgccgggt gtagcgcgct ccctccccct caccgatctg ctcgcgcatc cgcccgagga
     1681 tgatcacgcc gaccacggtc agcagcggga tgaacgggtt gaggaagcgg gcggcgtact
     1741 cgagcggcgc cagcagcgcg atcgtgaggc ccagggcgat cagcaccgac gcgcagtagg
     1801 ccgactcgcg gccgacgccc gtgaggcgcc ccatggccgt ccagacgaac ggcgccagcg
     1861 cggccagcgg cagcaggagc gcctgaacga ggaagtggta gagattcttg agacccttcc
     1921 accccacctc gcccaggtgc ccgaacgccc agccgaaggc cgagcgctgc ccgatgtcgg
     1981 tcatccgcga cgacgtccat cccgggtaac tggcggtgtc gatgtagagg aggctcgacg
     2041 tgccgaacca gggatgcccg gtgacgagaa aattgcggac cgtccacggc agcgtgatgg
     2101 cgatggctgc agccagaaag gcccatatgc gccgttcccg gtccccgcgc tcaccggccc
     2161 agaggagcca gatcatggcc ggcaacacga agagcgcctc gccccgcagg agcgtaagca
     2221 gcccgaaggc cagcccggcc acgatcgcgc gcacgcgcgt ctgcgcgtgg agcaccgcgc
     2281 gcacggccag cgccaggagc gccgcgtaga gcgtggtggc cagccccggt tcgtcgagcc
     2341 cgagcatggc cggcgagagg gccacgacga ggcccgaaag gagcgcccag cgccgcccgg
     2401 tgaactcgag cgcgaggaag taggtggcca gtatggcggc caggtaagcc acgatgccga
     2461 tgacgatcgg tgtccagacg gcgccgccgg tgagcacgag gccgccggcc acgacgagcg
     2521 gataaagggg tgggcgccac aggagcggaa atccgccggc cgcatcgccc gtcccggagg
     2581 ccaggtagaa gatggggtac gtgaaccgtg aggtgaaccc gcgcccttcg gcgatctgcc
     2641 ggccgagctg caggtagtcc cagtagtcgc tgtagccggg gatgacctcg ggatcgaagc
     2701 cgcgccacga caccgcggcc aggatgatgc cgaggccgac gagcagcgcc aggtgccacg
     2761 gttttggcgt tctcaatcgc gccccaggaa acgaagcagc cgtcggcggg ccaggcggat
     2821 ccggccgaac cagggagact cgcggaacca ggag
//
LOCUS       DASVOD010000034         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_888668, whole genome shotgun sequence.
ACCESSION   DASVOD010000034 DASVOD010000000
VERSION     DASVOD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888668"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /gene="atpD"
                     /locus_tag="VF720_00680"
     CDS             <1..778
                     /gene="atpD"
                     /locus_tag="VF720_00680"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7877888.1"
                     /translation="VFGQMNEPPGARLRVALSGLTQAEYFRDEQGKDVLLFVDNIFRF
                     TQAGSEVSALLGRIPSAVGYQPTLSTEMGGLQERITSTKRGSITSVQAIYVPADDLTD
                     PAPATTFSHLDATTVLSRQIAELGIYPAVDPLDSTSRMLDPNIVGQEHYDLSRDIQLI
                     LQRYKDLQDIIAILGMDELSNDDRLTVSRARTIQRFLSQPFFVAEAFTGSKGKYVKLE
                     DSIKGFKQIVDGKCDDLPEQAFYMVGTIDEVFEKAASMKG"
     gene            783..1202
                     /gene="atpC"
                     /locus_tag="VF720_00685"
     CDS             783..1202
                     /gene="atpC"
                     /locus_tag="VF720_00685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX7877889.1"
                     /translation="MADALHITVLTPERAVLDQDVYSVTAPGAAGYLGILKNHAALIT
                     SLVPGKLTVKDLRQQPTVYAIGGGFLEVAHNRVTVLADTLESVDDINLAEAQEAKKAA
                     QRRLELAKTPADKEHARAELDRHENRINVKRNPGPSV"
     gene            1267..1965
                     /locus_tag="VF720_00690"
     CDS             1267..1965
                     /locus_tag="VF720_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018200354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7877890.1"
                     /translation="MFWHQFTTGVATRYPHPMILEYLTVGPFQENSYILGDEETKDAI
                     LIDPGHGAEAILKRVEALGLKVMAIVNTHAHIDHVGGVEAVQKALGVPFLLHPDDLPL
                     LEALPKQAAMFGLPPIVVPRVDRHLEDGGRFKVGKIEIEIIHTPGHSPGSVSFWIGPR
                     DLIGGDVLFNGSIGRTDLPGGDHDHLLETIRKKLFNLPDDTRVWSGHGPETTIGEEKK
                     FNPFVGETPRVTEW"
     gene            complement(2049..>2210)
                     /locus_tag="VF720_00695"
     CDS             complement(2049..>2210)
                     /locus_tag="VF720_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HEX7877891.1"
                     /translation="LGGFTTFSAFGLETFALLKDGRWAAAGTYVALSNALGLSAVWVG
                     FRLMRGAGG"
BASE COUNT          414 a          762 c          683 g          351 t
ORIGIN      
        1 ggtcttcggc cagatgaacg agcctccggg agcccgtctg cgcgtggctc tctcgggcct
       61 cacccaggct gagtacttcc gcgacgagca ggggaaggac gtcctcctct tcgtggacaa
      121 catcttccgc ttcacgcagg cgggctccga ggtgtcggcg ctcctcggcc gcatcccgtc
      181 ggccgtcggt taccagccga ccctctccac cgagatgggc ggcctccagg agcgcatcac
      241 ctccaccaag cgcggctcga tcacctcggt gcaggccatc tacgtgcccg ccgacgacct
      301 gaccgatccg gccccggcca ccacgttcag ccatctcgac gccacgacgg tgctctcgcg
      361 ccagatcgcc gagctcggca tctacccggc cgtcgatccg ctcgactcca ccagtcggat
      421 gctcgacccg aacatcgtcg gccaggagca ctacgacctc tcgcgtgaca tccagctgat
      481 cctccagcgc tacaaggatc tccaggacat catcgccatc ctcggcatgg acgagctgtc
      541 gaacgacgac cgcctcaccg tctcgcgcgc gcgcacgatc cagcgcttcc tctcccagcc
      601 cttcttcgtg gccgaggcct tcaccggctc gaaggggaag tacgtgaagc tcgaggactc
      661 gatcaagggg ttcaagcaga tcgtggacgg gaagtgcgac gacctgcccg agcaggcgtt
      721 ctacatggtg ggcaccatcg acgaggtctt cgaaaaggcc gcgagcatga agggctaggc
      781 tcatggccga cgcgctccac atcacggtgc tcacgcccga gcgggcggtg ctggaccagg
      841 acgtctactc cgtcacggct cccggcgccg ccggctatct cggcatcctg aagaaccacg
      901 ccgcgctgat cacgagcctg gttcccggca agctgacggt gaaggacctt cgtcagcagc
      961 cgaccgtgta cgccatcggc ggcggcttcc tcgaggtggc ccacaaccgg gtgacggttc
     1021 tcgccgacac gctcgaatcc gtcgacgaca tcaacctggc cgaggcccag gaggcgaaga
     1081 aggccgccca gcgccgtctg gaactggcca agacgccggc cgacaaggaa cacgccaggg
     1141 cggagctaga ccggcatgag aaccggatca acgtgaagag gaatccgggg cccagcgtat
     1201 agggggactg gcgggatctg aattgaaatc agtgtggccg actcggttcg aaccgagtcg
     1261 gccactttgt tttggcacca gttcacgacc ggcgtcgcca cgcgctatcc tcatcccatg
     1321 atcctcgaat acctgaccgt cggccccttc caggaaaact cctacatcct cggggacgag
     1381 gagacgaagg acgccatcct gatcgacccg ggccacggcg ccgaggcgat cctgaagcgc
     1441 gttgaggctt tggggctcaa ggtcatggcc atcgtgaaca cccacgccca catcgaccat
     1501 gtgggcgggg tggaggccgt gcagaaggcg ctcggcgtgc cgttcctgct gcatcccgat
     1561 gacctgccgc tcctcgaggc cctgccgaag caggccgcca tgttcggcct cccgccgatc
     1621 gtggtgcccc gcgtcgatcg tcatctggaa gatggtggac gcttcaaggt ggggaagatc
     1681 gagatcgaga tcatccacac gccggggcac tcgcccggga gcgtgtcgtt ctggatcgga
     1741 ccgcgcgacc tgatcggcgg ggatgtcctc ttcaatggct cgatcggacg tacggacctg
     1801 cccgggggcg atcacgacca cctgctcgag acgatccgga agaagttgtt caacctgccg
     1861 gacgacacgc gcgtctggtc ggggcatggg cccgaaacga cgatcggcga ggagaagaag
     1921 ttcaacccat tcgtcgggga gacgccgcgg gtgacggagt ggtgaggctt cggggcctga
     1981 tcaacgggtt ccggaacctg ttgagcggcc gactcgtttc ccaaacgggt tcgcttacct
     2041 gagccgaatc agccgccggc ccctcgcatc agtcgaaacc ccacccacac cgccgacagc
     2101 cccagcgcgt tactgagagc cacataggtt cccgccgcgg cccagcgccc gtctttcaac
     2161 agcgcgaacg tctccagacc gaatgccgaa aaggtcgtaa acccgccgag
//
LOCUS       DASVOD010000035         9682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_784599, whole genome shotgun sequence.
ACCESSION   DASVOD010000035 DASVOD010000000
VERSION     DASVOD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9682
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784599"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..1279
                     /locus_tag="VF720_00700"
     CDS             383..1279
                     /locus_tag="VF720_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877892.1"
                     /translation="MVADGPVDESPIRELSLDAARRLDIDFVDIGVVQRSVLEARRLD
                     TVYAFELKAGHQVLDGPPDILAGMPSIDPSLLPLIEATRLLVNRGWGLVWARLHLEEA
                     RLYPAGFDRLRRRFTINAIHKGVLAAGEAGLILAKRYDLSYRERARRLAELPLEWLGI
                     GASDFRRAFADSTRFKLAPIVDDDAPDTLVDRWMTVRDWHERTLRAVEEARIGRPVDA
                     WSGWRRTVVGEGWRATLRRPRRFLSQRSQGVAGGGFGHLGLDRDHDFRRRLPAILYGI
                     GSERLPSDWREDARRHLDAWHS"
     gene            1276..2448
                     /locus_tag="VF720_00705"
     CDS             1276..2448
                     /locus_tag="VF720_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7877893.1"
                     /translation="MTRPDTLVVLVIDALGAELAETSGFLAARLPHRQRLRTILGYSS
                     AAIPSLLTGNTPKEHGHWFLYLRAGVGRPSPFRGASFLSKMPARLANRWSVRRRLASW
                     WRRRADIRGYFGLYEVPYRELADMDYVEREDGWGPGTFPHGSFIDDVTSSGLASFVSD
                     WRATDDAKLEAALAREHSAPADVYLMYLTEIDARQHAHGSRGRPVFERLESYRAPLDQ
                     LLDSAARRGTTRLVVVSDHGMTDVTTLLDPEPALKATGLHDGRDYRIFIDSTFLRFWC
                     DRPAVADRLVEVLGTTRGLRHVTPEELAREGVDFDDDRYGALHFLADPGVLICPSYMG
                     RTPLMGMHGYTPDDAASDAMLLDETAPGAPLASILNLRDHFRSLLPAGQGRAEPGA"
     gene            2445..4022
                     /locus_tag="VF720_00710"
     CDS             2445..4022
                     /locus_tag="VF720_00710"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX7877894.1"
                     /translation="MTTATRRVPKHTATVGAVGGALGWSVITRLLQFGLSLIGSIVVT
                     RTLGPDNYGQLTILRTALAFVTALCGLGLGQAVLRYLPTARARGDVQMAGRVMKLVIL
                     PQLVAWLLAIGLMALAHDWIVAHYYPQLADLFLLGAVLAGAELAFLATNNLTTAFYDT
                     RSLSIVILGGAVAYLALAVLALGRGFGVAGVMIATGLSQALMAAVLARRLARLLGELP
                     STPPVAAEADARAALNSRDLYRYALPFAAIAVMNLLVWRQSESLLLAHFHTKREAGFW
                     DLAYRMPQMILEFVPGAIWPLLMAGFSEIYTRDRSALSRAMRVYYKLLFLLVVPIAIG
                     GAAVGDRAIVAFYGEEFLPAGVYCQLLFLVFSMSFFSTPMTMAFYVLERPWYSFWQYL
                     GNAIVLVGLDFLLIPKLGLTGALIPMTLIIAASPFVNAFLLRRLGVRPQIPWAFLGRV
                     LLAAIPCALIYPARLYVRGKVPVALSVLAAGLLYLVGLRVFRVLKEEEAELLRRSRLP
                     FASWLLRWLVRPEASGP"
     gene            4019..5005
                     /locus_tag="VF720_00715"
     CDS             4019..5005
                     /locus_tag="VF720_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7877895.1"
                     /translation="MMKKIQIAVSLLISVVCLYFAFRNIDWVETWEAIKRANYLYLLL
                     TVFVTLGSVWLRSFRWKFMLDPIRKVPVGGLYESTMIGFMANNVLPARLGEFVRAYAV
                     GRAHGVSKSAAFATIVIERAFDMLTLVFCLGLVLLLVQLSPGIKLMGIVAIVVTFLMF
                     GVLVFFRQRRDLVAKLVDRLTRSLPEASRSRGERLLHSFIDGLEVLARGGHIAAIIGL
                     SLLMWASVMLSFDLAMRAFGLPVPLYASMVLLVVVSLGLMIPSGPGFAGTFEAAMIWA
                     LLLFPGVSKEQAASCAIMYHATQFVPITAFGLYYLWKSNLSLTDASRDGGAG"
     gene            5034..7478
                     /locus_tag="VF720_00720"
     CDS             5034..7478
                     /locus_tag="VF720_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877896.1"
                     /translation="MRRLLPWIGPAAALLARAPVGDGPRSIAALIAIFLGVPLAASPL
                     LAAADAGRRAPIAAAGVAIALPFLVFTALGLAGVNLDLLLVAFLILQAGVAILSMRER
                     PHSSNDEARESQRDSSGTWRTAVLISCLATALLAARAGTPLGPYDDALDHVATIRHVA
                     ETRHVDFPGAFYGRDQPDGQDIRKGSVHLGLAMAVRLAHVDAVVLWRWAATMLAPLVL
                     VAFIALAGTLAPGRPLIAWLALPAWILIASDPTWLLKSPYGGHAGLALAWAAIAGILS
                     GWGALPSFLAGAALAGVHAYAPAQALVPLAGFALVKPRTGDDPMAPSWRSLGALAVGA
                     LVIEGGRLLVAAPSINPLSNQSMPWLMTQLGPIASPLQLLDWYGLVGVAAAAAVIVLS
                     FVDRRPATRYLLVTLIAPMLLLVNPLLFGAVSKIFGSVANKIALVWSPPLAAILLMAA
                     ATRPDALRATRAVAAVGTALVMFAAFPTLQSNLESVTVPAVTAPSSLSRAAIELIERN
                     TPPDAVIAADPITSYAIPAMTGRRTIVTLHQHAPPGDGRALERVAAGAALGSTCAASM
                     SLWHDEGARWVLLDERPGPRIDEYGAHRDPRNNGSLASRFDWRFHGGGGAEVAGVDSA
                     DGFKLLRPAGNSVPITPGPMPTSELTSSGVVQVVAGNVELAAAMLGPDRLVSRGATLE
                     IPAWWRRNGDAVAPYLEVQAHLRIENEQMGEAGGPFSKLRRRLLIEPKLGGSLRARGV
                     ELPFRGLCPVAEWPAGKWLGDTIQIIVPQYIVPGRYKVELSLEEVTLYPVLTLADLFS
                     DADRYEGPDLGWMTVE"
     gene            7475..9073
                     /locus_tag="VF720_00725"
     CDS             7475..9073
                     /locus_tag="VF720_00725"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX7877897.1"
                     /translation="MNTTTDKRPGRRFGLPPWLSIDNIWVQRVLGLALAIAVGFFLGD
                     QISKPQGRVIKGLVGIFVLYAAWRFPIPISLAAFVLLFPYPFPTVYASSNTIFVFLIT
                     AMWLAQIGLRTARPAGRTMLDLPILLLMGVYLLSFNAVTYAEQLKFGLINLSTILASI
                     FVFYLIVSAVRDEETLKRMMKIITIMSITCWLTAIWELVFPGRAIIPGWILSGAYYNP
                     EAIERGLRVGGPFQDFELFAEFNAMLLPVMIFQAARAEDRKQRLFFLGLVLLNFFTLM
                     ATVTRGATIALFAGMLYMLFLLRKTTPIKTLAIVAGLAIIVFSVVEFYLSNFTVSGSV
                     LDRLLGTKMVNGMPDSRTFWPDILARALEKPWVGHGPYYEFGEPGREKLKRFFWPHNG
                     YLYYLHTVGILGTAAFLWMLFRMLAGSFKDSIHKLDSADYPSSLLFAWHVSLVIFMID
                     QLKIEYLRSQYYQFWPWIVFGMLVATWNVVRSRAPVPRPYHPRLGSPVHRVRRSGRIP
                     LVPVPPPADNGGIPSVAAPGGVDR"
     gene            9102..>9682
                     /locus_tag="VF720_00730"
     CDS             9102..>9682
                     /locus_tag="VF720_00730"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7877898.1"
                     /translation="MSLTEVDLSRPAAPARANLPPITLLIPAYNEEKGIGPVLEQVKA
                     LGLPGEILVVDDGSTDKTFEAAQIPGITVLRHDVNRGYGQSLKTGILASKHDIVVITD
                     ADGTYPNEDIAELLSHMEHYDMVVGARTGKTVKIPLLRKPAKKFLNLLANYLAGVEIP
                     DLNSGLRVFRKSVAREFFSILPSGFSFTTTITLA"
BASE COUNT         1391 a         3340 c         3086 g         1865 t
ORIGIN      
        1 gcatcgtgct tgtggtccgc gccgcgcgca ccaagcggga agtcgtccag cgcgcgatcg
       61 aaaccatcga caagttccag ggacgcgtcc tcggggccat cctcaaccgc cagcagtacg
      121 tcatccccga cttcatctac cggcggctct gagcagcgcc acgaggcgag tcctgccgcc
      181 gcccttcact gccgaggcgc cgccggaagc ggaggcgcgg atcgccgccg acctcgattt
      241 cctcgttcga gcgctcgttc gcgccgtacc ggcaccaccg cgccgggcca tcctcctcat
      301 cgggggattc gggcggggcg aggggggagt ccttcgctcg cccgacggcg gatggcgggc
      361 cttcaacgac tacgatcttg tcgtggtggc ggacggcccg gtcgatgaat cgccgatccg
      421 tgagctctcg ctcgacgcgg cccgccggct cgacatcgac ttcgtcgaca tcggtgtcgt
      481 acagcgatcc gtcctcgagg cgcgccgcct cgacacggtg tatgccttcg agctgaaggc
      541 gggccaccag gtcctcgacg gcccgccgga tatcctggcg ggcatgccct ccatcgaccc
      601 gtcgctcctt ccgttgatcg aagccacgcg actcctcgtg aaccgtggct ggggcctcgt
      661 gtgggccagg cttcaccttg aggaggcccg gctctacccg gccggcttcg accggctccg
      721 gcgccggttc accatcaatg ccatccacaa gggtgtgctg gcggcgggag aggccggcct
      781 catccttgcc aaacgctacg acctctcgta ccgggagcgc gcgcgccgac tggccgagct
      841 ccccctcgag tggttgggga tcggcgcctc ggacttccgc agggccttcg ccgactcgac
      901 gaggttcaag ctggcgccga tcgtcgacga cgatgccccg gacacactgg tcgatcgatg
      961 gatgacggtt cgcgactggc acgaaaggac gctccgcgcc gtggaagagg cacgcatcgg
     1021 ccggcccgtc gacgcgtgga gcggttggcg ccgcacggtg gtcggcgagg ggtggcgcgc
     1081 cacgctccgg cggccgcgac gattcctgtc gcagcgttcc cagggggtgg cgggtggcgg
     1141 gttcggacat ctcgggctcg accgggacca cgacttccgg cggcgcctgc ccgcgatcct
     1201 gtacggaatc ggctcggaaa gactcccgtc ggattggcgc gaggacgccc gccggcatct
     1261 cgacgcgtgg cactcgtgac acgccccgac acgctggtcg tgctcgtgat cgacgcgctc
     1321 ggcgccgagc tggccgaaac gagcggcttc ctggccgccc gccttccgca ccgccagcgg
     1381 ctccgcacga tcctcgggta cagctcggcc gcgatcccga gcctgctcac cggaaacaca
     1441 ccgaaggagc atggtcactg gttcctctac ctgcgagccg gcgtggggcg gccgtcaccc
     1501 ttcagaggcg cgtcgttcct ctcgaagatg ccggccaggc tcgccaaccg gtggagcgtc
     1561 aggcggcgcc tggcttcctg gtggcggcgc cgggccgaca tccgggggta cttcgggttg
     1621 tacgaagtgc cgtaccgtga actggccgac atggactacg tcgagcgcga ggacggctgg
     1681 ggccccggga cattcccgca cggttccttc atcgacgacg tgacgagctc gggcctcgcc
     1741 tccttcgtat cggactggcg cgccaccgat gacgccaagc ttgaagccgc gctggcccgc
     1801 gagcactcgg cgccggccga cgtttatctg atgtacctta cggaaatcga cgcgcgtcag
     1861 cacgcgcacg gaagccgggg gcgcccggtc ttcgagcgtc tcgagtcgta ccgggcgccg
     1921 ctggatcaac tgctcgactc ggccgcgcgc cgggggacga cgcgcctcgt ggtcgtctcc
     1981 gaccacggga tgaccgacgt cacgaccctc ctcgacccgg agcccgcgct caaggccacg
     2041 gggctgcacg acggccgcga ctaccgcatc ttcatcgact cgacattcct ccgcttctgg
     2101 tgcgatcggc cggcggtggc cgatcgattg gtcgaagtcc tcggcaccac cagggggctg
     2161 cggcacgtca cgccggagga gcttgcgcgc gagggagtgg acttcgacga cgatcgctac
     2221 ggcgcgctcc acttcctggc cgaccccggc gtcctcatct gcccctcgta catggggcgc
     2281 accccgctca tgggcatgca cggctacacg ccggacgacg ccgcctcgga cgccatgctc
     2341 ctcgatgaga cggctcccgg ggcaccgctg gcctccatcc tgaacctgag ggatcacttc
     2401 cgctcgctgc ttccggccgg tcagggtcgg gcggagcctg gggcatgaca acagccacgc
     2461 ggcgcgttcc gaagcacacc gccaccgtgg gcgccgtcgg cggcgcactc ggctggagcg
     2521 tcatcacgcg ccttcttcag ttcgggctct cgctcatcgg ttccatcgtg gtgacgcgga
     2581 ccctgggccc ggacaactac ggccagctca ccatcctccg gaccgcgctg gccttcgtca
     2641 ccgcattgtg tggcctgggg ctcggccagg ccgtgcttcg ctacctgccc acggcgcggg
     2701 cccggggcga cgtgcagatg gccggccgcg tgatgaagct ggtcatcctc ccgcagctgg
     2761 tggcgtggct cctggccatc ggactcatgg cgctcgcgca cgactggatc gtggcgcact
     2821 actatccgca gctcgccgac ctcttcctgc tcggcgccgt cctggcgggt gcggagctgg
     2881 ctttcctggc caccaacaac ctcaccaccg ctttctacga cacccgcagc ctgtcgatcg
     2941 tcatcctcgg cggggccgtg gcctacctgg cgctggcggt gctggcgctg ggccgcggct
     3001 ttggagtggc gggagtcatg atcgccacgg gcctctcgca ggcgctgatg gcagccgtgc
     3061 tcgcccgacg gctggcgaga ctgctcggtg agttgccgtc aacgcccccg gtggccgcgg
     3121 aggccgatgc ccgcgcggcc ctgaacagcc gggacctcta ccggtatgcg ctcccgttcg
     3181 cggccatcgc cgtcatgaac ctgctggtct ggcgccaatc ggagtcgctc ctcctggccc
     3241 acttccacac caagcgggaa gcgggtttct gggacctggc ctaccggatg ccgcagatga
     3301 tcctcgagtt cgtgccgggg gcgatatggc cgctcctgat ggccggcttc tccgagatct
     3361 acacgcgtga ccggtcggcg ctctcgcgag ccatgcgcgt ctattacaag ctcctctttc
     3421 tcctggtcgt gccgatcgcg atcggcggcg ccgcggtggg ggaccgggcc atcgtggcct
     3481 tctacgggga ggagttcctc ccggcgggcg tctactgcca gctcctgttc ctcgtcttct
     3541 cgatgtcgtt cttctcgact cccatgacga tggcgttcta tgtcctcgag cgcccctggt
     3601 acagcttctg gcagtacctg ggcaacgcga tcgtcctggt cgggctcgac tttctcctga
     3661 tccccaagct ggggctcacc ggggcgctca tcccgatgac gctgatcatc gcggccagcc
     3721 ccttcgtcaa cgccttcctg ctccggcggc tcggcgttcg tccgcagatt ccatgggcat
     3781 tccttggccg ggtgctcctg gccgccatcc cctgcgccct catctatccc gcccggctgt
     3841 acgtgcgtgg taaggtgccg gtcgcgcttt cggtcctggc cgccggattg ctctacctgg
     3901 tcggcctccg cgtgtttcgt gtgctgaagg aggaggaggc cgagctgctc cgtcgatccc
     3961 gcctgccgtt cgcatcgtgg ctcctgcgct ggcttgtccg tcccgaggcg tccgggccat
     4021 gatgaagaaa atccagatcg cggtcagcct tctgatcagc gtcgtctgcc tctacttcgc
     4081 cttccgcaac atcgactggg tggaaacctg ggaggcgatc aagcgggcca actacctcta
     4141 cctcctgctc accgtattcg tgacgctcgg cagcgtatgg ctccgctcgt tccggtggaa
     4201 gttcatgctc gatcccatcc ggaaggtgcc cgtggggggg ctgtacgagt cgacgatgat
     4261 cggattcatg gccaacaacg tcctgccggc gcggctcggc gagttcgtca gggcctacgc
     4321 ggtggggcgt gcccacgggg tttcgaaaag cgcggcgttc gccacgatcg tgattgagag
     4381 ggcgttcgac atgctgacgc tcgtcttctg cctcggactc gtcctgctcc tcgtgcaact
     4441 gagtccgggc atcaagctga tggggatcgt ggctatcgtc gtcaccttcc tgatgttcgg
     4501 cgtcctggtc ttcttccgcc agcgccgcga cctcgtggcg aagctggtcg accggctcac
     4561 ccgttctctc cccgaagcct cgcgcagccg gggagaacgc ctgcttcatt ccttcatcga
     4621 cggcctcgag gttctggcca gagggggcca catcgcggcc atcatcggcc tctcgctcct
     4681 catgtgggcc tcggtcatgc tgagcttcga cctggcgatg cgcgccttcg gccttcccgt
     4741 gccgctctac gcgagcatgg tgctcctggt ggtcgtctcg ctcggcctca tgatcccttc
     4801 ggggcccggc ttcgccggca cgttcgaggc cgccatgatc tgggcgctcc tgctgtttcc
     4861 cggagtgtcc aaggagcagg ccgcctcctg cgccatcatg taccacgcca cccagttcgt
     4921 ccccatcacc gcgttcggtc tctactacct ctggaaatcc aacctctcgc tgacggacgc
     4981 ctcgagggac ggcggggcgg gctgaggaag cccgcaccac ggctcgtgac ctgatgcgcc
     5041 gactcctccc gtggatcggt cccgccgcgg cactgctcgc gcgggcgccg gtcggtgatg
     5101 gaccgcgatc gatcgctgcg ttgatcgcca tcttcctcgg ggtgccgctc gcggcctcgc
     5161 cgctgctggc tgccgctgat gcagggcgtc gggcgcccat tgcggccgcc ggagtggcga
     5221 tcgcactgcc gttcctcgtc ttcacggctc tgggattggc gggcgtcaat ctggatcttc
     5281 tgcttgtcgc cttcctcatt ttgcaggccg gagtcgcgat cctgtcgatg cgggagcgtc
     5341 cgcactcatc gaatgacgaa gctcgggaga gtcagcgcga cagcagcggc acttggcgta
     5401 cggcggttct catctcgtgc cttgccacgg cgctcctcgc cgcgcgcgcc ggcacacccc
     5461 tcggccccta tgacgacgcc ctcgaccacg tggccacgat ccgtcacgtg gcggaaacgc
     5521 ggcacgtcga cttcccgggc gcgttctacg gtcgcgacca accggacggc caggacatcc
     5581 ggaaggggag cgtgcacctt ggcctcgcga tggcggtccg cctcgcccac gtcgatgccg
     5641 tcgtcctctg gcggtgggcc gccacgatgc tcgcaccgct ggtcctcgtg gcgttcatcg
     5701 cactcgccgg aacactcgcg cctggccgac cactcatcgc ctggctcgcc ctgcccgcct
     5761 ggatactgat cgcaagcgac ccgacctggc tcctcaagtc gccctacggc ggtcacgccg
     5821 gcctggcgct ggcctgggcc gcgatcgccg ggatcctctc gggatggggc gctctcccgt
     5881 cattccttgc cggcgccgcg ctcgcgggtg tccacgccta cgcgcccgcg caagcactcg
     5941 tgcctctagc cggcttcgcg ctcgtcaagc ctcgtaccgg cgacgatccg atggcaccct
     6001 cctggcgttc gctcggtgcg ctggccgttg gcgcactcgt gatcgagggt ggacgcctgc
     6061 ttgtggcagc accttccatc aatccactga gcaaccaatc gatgccgtgg ctgatgacgc
     6121 aactcggacc gatcgcttcg ccactccaac tgctcgactg gtatggcctg gttggcgtcg
     6181 ccgcggcggc tgccgtgatc gtgctgtcgt tcgtcgatcg acgaccagcc acgcgctatc
     6241 tcctcgtcac gctgatcgcg ccgatgctgt tgctcgtcaa tccgcttctc ttcggagcgg
     6301 tgtcgaagat cttcggctca gtggccaaca agatcgccct tgtgtggtcg ccgccattgg
     6361 cggcgatcct cctcatggcc gcggcaacgc ggcctgacgc tcttcgcgca acgcgcgccg
     6421 ttgccgcggt gggaactgca ctcgtgatgt tcgcggcgtt tccgacactc cagtcgaacc
     6481 ttgaaagtgt cactgtacct gccgtgaccg caccaagctc cctgtcgcgg gccgccatcg
     6541 agctgattga gcggaacacg ccgcccgacg ccgtgatcgc ggccgacccg atcacgagct
     6601 atgcgattcc cgccatgacg ggacggcgaa cgatcgtgac gctgcaccag catgcaccgc
     6661 cgggggatgg gcgggcattg gaacgtgtag ccgcgggggc cgcgctgggg tcgacatgcg
     6721 cggccagcat gagtttgtgg cacgacgagg gcgcccgctg ggttctgctt gacgagcgtc
     6781 cgggtccgcg cattgacgaa tacggtgcgc atcgcgatcc gcgcaataac gggagtctgg
     6841 cctcgcggtt tgattggaga ttccacgggg ggggtggagc cgaagtggcc ggagtcgact
     6901 ctgcggacgg attcaagttg cttcgaccgg ccgggaattc cgttccgatc acacccggtc
     6961 cgatgcccac gtccgagctg acgtcttccg gtgtcgttca agtggtggcg gggaatgtcg
     7021 aactcgccgc ggccatgctc ggtcccgacc gcctcgtctc gcgtggcgcc acactcgaga
     7081 ttcccgcctg gtggcgccgt aatggagacg cggttgcgcc ctacctcgag gtacaggccc
     7141 acttgcgtat cgagaacgag caaatggggg aggcaggagg accattttcc aagctccgcc
     7201 gccgcctgct gatcgagccg aagctcggcg gcagtctgcg tgcccgtggc gtggagctac
     7261 ccttccgcgg gctctgtccc gtggccgaat ggccggcggg caagtggctg ggtgacacca
     7321 tccaaatcat tgtgccacaa tatattgtcc cgggccgata caaggtggag ctctcgctgg
     7381 aagaggtgac gctttaccct gtgctgaccc tggccgatct attctccgat gcggaccgat
     7441 acgaagggcc cgacctgggc tggatgaccg tcgaatgaac accacaaccg acaagcgccc
     7501 cgggcgccga ttcgggctgc ccccctggct gtcgatcgac aatatctggg ttcagcgtgt
     7561 gctgggcctg gccctggcca ttgccgtcgg gtttttcctt ggcgaccaga tttcgaagcc
     7621 gcaggggcgc gtcatcaagg ggctggtcgg catcttcgtg ctttatgccg cctggcgttt
     7681 cccgattccg atctcactgg cggccttcgt gctgcttttc ccgtacccct tcccgacggt
     7741 ctacgcctcc agcaacacca tcttcgtctt cctcatcacg gccatgtggc tggcccagat
     7801 cggccttcgg acggcgcgcc ccgccggtcg gacgatgctc gacctgccga tccttctgct
     7861 catgggcgtt tacctgctgt cgttcaacgc ggtcacgtat gccgagcagc tcaagttcgg
     7921 tctcatcaac ctgagcacga tcctggcgtc gattttcgtc ttctacctga tcgtgagcgc
     7981 ggtgagggac gaggagacgc tgaagcggat gatgaagatc atcaccatca tgagcatcac
     8041 ctgctggctc accgccatct gggaactggt ctttccgggg cgggccatca tcccggggtg
     8101 gatcctctcc ggcgcctatt acaacccgga ggccatcgaa cggggacttc gcgtgggtgg
     8161 cccattccag gatttcgagc tcttcgccga attcaacgcc atgctgctgc cggtcatgat
     8221 tttccaggcc gcgcgcgccg aggacaggaa acagcggctg ttcttcctcg gcctggttct
     8281 gctcaatttc ttcacgctga tggctaccgt cacccgcggc gccaccatcg cgctgttcgc
     8341 ggggatgctc tacatgctct tcctgctccg caagacgacc ccgatcaaga cgctggcgat
     8401 cgtggccggt ctggcgatca tcgtcttctc cgtggtcgag ttctacctct cgaatttcac
     8461 ggtctcgggc tcggtgctcg atcgactgct gggaaccaag atggtgaacg ggatgcccga
     8521 ctcgcggaca ttctggccgg acatcctggc ccgggccctc gagaaaccgt gggtcggtca
     8581 cggtccctac tatgaattcg gcgagcccgg tcgcgagaag ctaaagcggt tcttctggcc
     8641 gcacaacggc tatctctact acctgcacac ggtcggcatc ctggggacgg ccgccttcct
     8701 ctggatgttg tttcggatgc tggcggggag cttcaaggac agcatccaca agctcgacag
     8761 cgccgactat ccgtcgtcgc tcctttttgc ctggcatgtc tcgctggtca tcttcatgat
     8821 cgaccagctc aagatcgagt atcttcgcag ccagtactac cagttctggc cctggatcgt
     8881 gttcgggatg ctcgtggcca cctggaacgt cgtgcgctcg cgggcaccgg tgccccggcc
     8941 gtaccaccca cgtttgggga gcccggtcca tcgggtcagg cggtcgggtc gaattccgct
     9001 ggttccggtt ccgccgccgg ccgataacgg agggattcct tcggtggccg cgccgggtgg
     9061 tgtggatcgg tgagggggat attgagcatg gagaaagccg catgtcgttg actgaagtcg
     9121 atttgtcccg tccggccgcg ccggcccggg ccaatctccc cccgatcacc ctcctgatac
     9181 ccgcttataa cgaggagaag gggatcggac cggtcctgga gcaggtgaag gcactcgggc
     9241 tcccggggga gatcctggtc gtggatgacg gctcgaccga caagaccttc gaggcggccc
     9301 agatcccggg gattactgtc ctccggcacg acgtgaaccg ggggtacgga caatcactca
     9361 agaccggaat ccttgcatcc aagcatgaca tcgtcgtaat taccgatgcc gacggcactt
     9421 atcccaacga agacatcgct gaattgttga gtcacatgga acactatgat atggttgtcg
     9481 gtgcccgaac cggcaagacg gtgaagatcc ccctcctccg aaagccggcc aagaagtttc
     9541 tgaacttgtt ggccaactac ctcgctggcg tggagatccc cgatctgaat tccgggctcc
     9601 gggtgttccg gaaatcggtg gcgcgggagt tcttcagcat ccttccctcc ggtttctcgt
     9661 tcacgaccac catcacgctg gc
//
LOCUS       DASVOD010000036         2085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_2358, whole genome shotgun sequence.
ACCESSION   DASVOD010000036 DASVOD010000000
VERSION     DASVOD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2358"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF720_00735"
     CDS             <1..502
                     /locus_tag="VF720_00735"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011231937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7877899.1"
                     /translation="YITNLNKALESAPEWAEKPLEEILAKLEKLPEGIRTAVRNNGGG
                     HHNHSLFWTIMGPGKGGAPSGALAEAINAAFGSFDAFTEKFAAAGATRFGSGWAWLVK
                     DGHKVEVYSTPNQDSPIMDGKTPLLGCDVWEHAYYLKYQNRRPDYMAAFWNVVNWDEV
                     ARRFGS"
     gene            499..1323
                     /locus_tag="VF720_00740"
     CDS             499..1323
                     /locus_tag="VF720_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877900.1"
                     /translation="MTARFGLTVLMAASLTVFAGSVRADVTLVYDVDVERPEKIEKKV
                     TWHLAETRLALVTGGDKAARVFYSVKDSTLTLVDDTKKQYVVLTPENVATLSTMLAGM
                     ASQMEQVLAGLSPEQREKVAGEMKKRGLEPGEKKSLTIEVLPMSETKNMHGFTCRKNE
                     VLANGAKAAEVWTTALGPNTVTAAEWAPVNAMVTSLHQVFDAVSGLTKAFDVEMSLPS
                     SLGRGLEGVPVYVKDLEGDAVKGEMTLTSLKRGEIDDDVFEIDDDYKKQELTPAEH"
     gene            complement(1376..>2085)
                     /locus_tag="VF720_00745"
     CDS             complement(1376..>2085)
                     /locus_tag="VF720_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7877901.1"
                     /translation="AFTRALAPGDVRGIVIVQPNNPTGSMLDDSELDFLFEQAHAAGA
                     TVIADEVFLDFPAPGRELTTLAAGATVPLLVLSGLSKCAGLPQMKLGWMVAAGPPAPA
                     EQLMQGLEWIADAFLSVGAPVQAAGALFLAGRHDFIAATRARCDANEAYLDRLLTGTP
                     VTRLPREGGWSIILRLPATRTDDAWCFELLEAGVVAHPGHYYDVMKESLLVLSLLAPE
                     NEWADGLERLLGVVGPG"
BASE COUNT          400 a          648 c          727 g          310 t
ORIGIN      
        1 gtatatcacc aacctgaaca aggcgctgga atcggcgccg gaatgggcgg agaagccgct
       61 cgaggagatc ctggccaagc tggagaagct accggagggg atccgcaccg cggtgcgcaa
      121 caacggcggt gggcatcaca atcactcgct cttctggacg atcatgggcc ccggcaaggg
      181 tggcgcgccg tccggcgcgc tggccgaggc catcaatgcc gccttcggca gcttcgatgc
      241 tttcacggag aagttcgccg ccgcgggcgc cacgcgcttc ggctccggct gggcctggct
      301 ggtgaaggat ggccacaagg tcgaggtgta ctccacgccg aaccaggact cgccgatcat
      361 ggacggcaag acgccgctcc tcggctgcga cgtgtgggag cacgcctact acctcaagta
      421 ccagaaccgt cgccccgact acatggccgc cttctggaac gtcgtgaact gggacgaagt
      481 ggcccgccgg ttcgggtcat gactgcccgg ttcggcctca cggtgctgat ggccgcgagc
      541 ctcaccgtgt ttgccgggtc ggtacgggcg gatgtcacgc tcgtttacga cgtcgatgtg
      601 gagcgcccgg agaagatcga gaagaaggtc acctggcacc tggcggagac gcgcctcgcg
      661 ctcgtgaccg gcggggataa agccgcacgc gtcttctaca gcgtcaagga cagcacgttg
      721 accctggtcg acgacacgaa gaagcagtac gtggtgttga cgccggaaaa cgtggccacg
      781 ctctccacga tgctggccgg aatggccagc cagatggagc aggtcctggc cgggctctct
      841 cccgagcagc gcgagaaggt tgccggggaa atgaagaagc ggggcctcga gcccggcgag
      901 aagaagagcc tcacgatcga ggtcctgccg atgagcgaaa cgaagaacat gcacggcttc
      961 acctgtcgca agaacgaggt gctggccaac ggcgccaagg cggctgaagt ctggacgacg
     1021 gcgctcggcc ccaacacggt caccgcggcc gagtgggcgc cggtgaacgc gatggtgacg
     1081 tcgctgcacc aggtgttcga tgcggtctcg ggcctcacca aggccttcga cgtggaaatg
     1141 tcgttgccat cctcgctggg acggggcctc gaaggcgtgc ctgtctacgt gaaggatctc
     1201 gagggagatg ccgtgaaggg cgagatgacg ctcacctcgc tgaaacgggg cgagatcgat
     1261 gacgacgtct ttgagatcga tgacgactac aagaagcagg aactgacgcc ggccgagcat
     1321 tgaatcgcgc cgacaaggca atcaatccgg tcggccgatc ccgccgaacg gtgactcatc
     1381 ccgggccgac gaccccgagc agccgctcga ggccgtcggc ccactcgttt tcgggcgcga
     1441 ggagcgacag gacgagcagt gattccttca tgacgtcgta gtagtggccc gggtgggcca
     1501 ccacgccggc ctcgaggagt tcgaagcacc aagcatcgtc cgtccgcgtg gcggggaggc
     1561 gcaggataat gctccaccct ccttcccgcg ggaggcgggt aaccggcgtg ccggtgagaa
     1621 gacggtcgag gtacgcctcg ttggcgtcgc accgcgcgcg cgtggcggcg atgaaatcgt
     1681 gccgacctgc caggaacaac gcaccggcgg cctggaccgg cgcgccaacc gaaaggaacg
     1741 cgtcggcaat ccattcgagg ccctgcatca actgttcggc cggggcaggt gggccggccg
     1801 ccaccatcca gccgagcttc atctgcggga ggccggcgca cttggacagc ccggaaagca
     1861 cgaggagcgg caccgtggca ccggccgcca gcgtggtgag ttcgcgtccc ggtgccggaa
     1921 aatccaggaa gacttcgtcg gcgatgaccg tggcgccggc cgcgtgagcc tgttcgaata
     1981 ggaaatccag ctcggaatcg tcgagcatcg aaccggtcgg gttgttcggt tgcacgatga
     2041 cgatgccgcg cacgtccccg ggggccagcg cgcgggtgaa ggcct
//
LOCUS       DASVOD010000037         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_941417, whole genome shotgun sequence.
ACCESSION   DASVOD010000037 DASVOD010000000
VERSION     DASVOD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941417"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VF720_00750"
     CDS             complement(<1..1205)
                     /locus_tag="VF720_00750"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HEX7877902.1"
                     /translation="MNRPKRRHLWPELAYNPITALGTFLALFALAAIVIFYFLSLRGE
                     GDNPYTGIFTLVVFPGVLVIGLLLIPAGMLLEKRRQEHGHARPFVVDLKNPAHLRALV
                     VFGAGTAVFMLATTIGLYETYHFTESVQFCGSTCHVMTPEKTAHAASSHARVACVHCH
                     VGPGGDDYLKSKMAGARQLWHFAMDDYHRPIETPVYSLRPARETCEECHWSTRTHPPT
                     VIVRDHYLSDRENRHWRVELLFNTAMGDSMTPSPAAPGGAATAAGIHWHVAAGNTVTY
                     VASDSSRQSFDQVSWSGPDGKTVTYRRPGAGLSAADLERKETKGLTRTMDCLDCHNRP
                     AHRYLSPVESVNRALTAGKLDASIPWIKREAVRALTGRWESSASADTAIAGRLERFYG
                     REGITLPAGT"
     gene            complement(1202..>1995)
                     /locus_tag="VF720_00755"
     CDS             complement(1202..>1995)
                     /locus_tag="VF720_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877903.1"
                     /translation="LASDARVLAQRDRITPRWPHPADHNQAGWTRHHGAASEKEHDRC
                     TSCHRQSSCTSCHGDGPASRAAIARFAADDAPGPAGVTGNSKRPPIHPPGFRMAHGNA
                     AASRANCASCHDTGKFCSDCHEGTVASGFHPPGYLNRHAADAFARDLDCAGCHSNEVS
                     CRACHESAGLAGFASTTSSYHDAQPFWLLSHGQAARQGLDTCASCHAQTDCLQCHSAR
                     SGWRISPHGPGFEAERLSERGPVMCSLCHVSNPVPEAGRERVAPR"
BASE COUNT          299 a          636 c          741 g          319 t
ORIGIN      
        1 gtgccggccg gtagcgtaat cccttcgcgt ccgtagaatc gctccagccg gccggcgatg
       61 gcggtgtcgg ccgaggcgct cgattcccac cgtccggtca gggctcgtac cgcttcgcgc
      121 ttgatccacg ggatcgacgc atcgagcttc ccggcggtga gtgcccggtt caccgattcg
      181 acgggggaga ggtaccggtg ggccgggcga ttgtggcagt cgaggcaatc catggtgcgg
      241 gtgagcccct ttgtctcctt ccgctcgagg tcggccgccg aaagccccgc acccgggcgc
      301 cggtaggtca ccgtcttccc gtccgggccc gaccaggaaa cctggtcgaa cgactgtcgt
      361 gacgagtcgg aagccacgta cgtgaccgtg tttcccgccg ccacgtgcca gtggatgccg
      421 gcggccgtgg ccgcgccgcc gggtgccgcc ggcgaaggcg tcatcgaatc gcccatggcc
      481 gtgttgaaga ggagctccac ccgccagtgg cggttctccc ggtcactcag gtaatgatcg
      541 cgcacgatca ccgtcggtgg atgggtgcgg gtggaccagt ggcactcttc gcaggtttcg
      601 cgcgccggac ggagcgagta gaccggcgtc tcgatcgggc ggtggtagtc gtccatggcg
      661 aagtgccaga gctgacgcgc accggccatc ttgctcttca ggtagtcgtc gccgccgggt
      721 cccacatgac agtggacgca cgccacccgg gcatgggatg aggcggcatg ggcggtcttc
      781 tccggtgtca tcacgtggca cgtcgatccg cagaactgga ccgactccgt gaaatggtat
      841 gtctcataga gcccgatcgt cgtggcgagc atgaacacgg cggtaccggc gccgaacacc
      901 acgagcgccc ggaggtgcgc cgggttcttc aggtcgacga cgaagggacg ggcatgccca
      961 tgttcctgcc gccgcttctc gagcagcatc cccgccggga tgagcaggag cccgatcacc
     1021 aggacgccgg gaaagacgac cagcgtgaag atgccggtgt acgggttgtc tccctcgccg
     1081 cgcaggctca ggaagtagaa gatgacgatc gccgccaggg caaacagggc caggaaggtc
     1141 cccagggccg tgatcgggtt gtaggccagt tccggccaga gatgccggcg tttcggtcga
     1201 ttcatcgggg agccaccctt tcccggcctg cctcgggcac cgggttcgag acgtgacaga
     1261 gggagcacat gaccgggccc cgttccgaga ggcgttccgc ctcgaagccc gggccgtgag
     1321 gactgatccg ccagccgctc ctggccgaat ggcactgcag gcagtcggtc tgggcatggc
     1381 acgacgcgca cgtatcgagc ccctggcggg cggcttgccc gtgtgacagg agccagaagg
     1441 gctgggcgtc gtggtacgac gaggtcgtcg acgcgaaacc ggccaggccc gcgctttcgt
     1501 gacaggcccg gcacgacact tcgttcgaat ggcacccggc gcaatccagg tcgcgcgcga
     1561 aggcgtcggc cgcgtgacgg ttgaggtatc ccggcgggtg gaagccggag gccaccgtcc
     1621 cctcgtggca gtccgagcag aacttgccgg tgtcgtggca ggaggcgcag ttggccctcg
     1681 aggcggcggc gttgccgtgt gccatccgga agcccggggg gtggatcggt gggcgcttcg
     1741 aattgccggt cacgccggcc gggccgggcg cgtcgtcggc ggcaaagcgt gcgatggccg
     1801 cgcgggaggc cgggccgtcg ccgtggcagc tggtgcagct cgactgccga tggcagctcg
     1861 tgcagcggtc gtgctccttt tcggaggcgg cgccgtgatg ccgggtccag ccggcctggt
     1921 tgtggtcggc cgggtggggc cagcggggag tgatgcgatc ccgttgcgcg aggacgcgcg
     1981 cgtcggaggc cagcg
//
LOCUS       DASVOD010000038         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_106573, whole genome shotgun sequence.
ACCESSION   DASVOD010000038 DASVOD010000000
VERSION     DASVOD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106573"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VF720_00760"
     CDS             <1..440
                     /locus_tag="VF720_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877904.1"
                     /translation="TASADSRDRSFGYYGWGPRVGVSVDPDQIVVGAHLDMGEPITQI
                     RFQPNVEFGFGDDATVFEFNAPLHFRFSEPIDVWSPYVGAELGWAFASYDDAKDTSDL
                     GVKIVGGFERHISGGDKFFIEGKVGLSDEVSDFKFLVGWTFVK"
     gene            complement(534..1283)
                     /locus_tag="VF720_00765"
     CDS             complement(534..1283)
                     /locus_tag="VF720_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7877905.1"
                     /translation="MSGHSKWATIKRKKAGIDAARGKSFTKVIKEITIAARSGGGDPA
                     GNPRLRTAIAAAKAVNMPADNIDRAIKKGTGTLEGVNYEEIVYEAYAPHGVALMIEST
                     TDNRNRTSGELRHILSKYGGNLAEPGAVGWNFQQKGVIVIDAGKVDEDTAMTVALESG
                     AEDFSNEGETYEVITTMPDFHAVREALEGKGLPVAEASLQRLPNTTVALSEKEAPSVL
                     KVMEMLEDNDDVQKVFANFDISDEIMAKLDA"
     gene            complement(1290..3374)
                     /gene="fusA"
                     /locus_tag="VF720_00770"
     CDS             complement(1290..3374)
                     /gene="fusA"
                     /locus_tag="VF720_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX7877906.1"
                     /translation="MKEFRTDQIRNIVFVGHGGTGKTSLIESMLFDAGEINRIGSVTD
                     GTTVMDHSPDETKRKISINLGLGHAEWRDTRLNLIDTPGYDDFQGDMLAGIRVADGAV
                     LVISGLSGVESGTQRAWEALQARELPVLIAANMLDKEHADFHKVIDSARAILSDKVMP
                     LQVPLGKGATFAGFIDLFTMKAYSMGADGQPKEVEVPADARPEAEEAREKLIEAVATF
                     DDALVEKYLEGQELTIEEELAALKKGVMQKGIYPVVMTSATHNTGIRRLLDTLVVCLP
                     SPHEAPTGTAIDTATGQEVTLVPDSSKPLCALVFKTLSEAHVGDLSLIRVYEGKLEHG
                     VEVNNVTAGHSEKIGSLFLLQGKERKEVHSVGAGDIVAAVKLRGTHTGNTLTVKGHPL
                     ELPGIDYPEPILAEAIFPKVKGEEDKVAQALHKIHEEDPTVRTVVDGELHQQLLYGMG
                     ELQLAIVVDRLKRKYGVDVELRKPRIPFRETLRGKAEAQGRHKKQTGGRGQFGDTWIR
                     LEPNTRGGGYEFVDAVVGGVVPGKFIPAVDKGIQEAAERGILAGYRVVDFKATLYDGS
                     HHSVDSSEAAFKMAGIIGFHAAAEKCKPVLLEPIVEIEVTVPDDHMGDVMGDLSSKRG
                     KILGMNPAPGGQSVKALVPQAELYRYSTHLRSLTQGRGRFTMKFSSYEEVPRDSAEKI
                     IEEAKAAREKES"
BASE COUNT          562 a         1159 c         1152 g          737 t
ORIGIN      
        1 ccacggcttc ggccgacagc cgtgatcgca gcttcggata ctacggatgg ggcccccgtg
       61 tcggcgtgtc ggtcgacccc gaccagatcg tggtgggggc ccatctcgac atgggcgagc
      121 cgatcaccca gatccgcttc cagcccaacg tggaattcgg cttcggcgac gacgccaccg
      181 tcttcgaatt caacgcccca ctccacttcc gcttctccga acccatcgac gtctggagtc
      241 cctacgtggg ggcggaactc ggctgggctt tcgcctccta tgacgacgcg aaagacacgt
      301 ccgacctggg cgtcaagatc gtaggcggct tcgaacgcca catcagcgga ggagacaagt
      361 tcttcatcga gggaaaagtg gggctctcgg atgaagtgag cgacttcaag ttcttagtgg
      421 gctggacctt cgtgaaataa ggcgagcaag gccgtacacg cgcccgcgcc ggcaccaagc
      481 ttccgacaag tgtacttccg caggcgggag cttggtgccg gcgcgggcgc tatttatgcg
      541 tccagtttcg ccattatttc atcggagatg tcgaagttcg caaagacctt ttgaacgtcg
      601 tcgttgtctt cgagcatctc catcaccttg agcaccgaag gagcctcttt ctcactcagc
      661 gccaccgtgg tgttcggaag tcgctgcaag ctggcttccg ccacgggaag gccttttcct
      721 tccagcgcct cacgcaccgc gtgaaagtcc ggcatcgtcg tgatgacttc gtaggtctcg
      781 ccttcattac tgaaatcttc ggcccctgat tcgagggcga cggtcatggc cgtgtcctca
      841 tcgaccttgc cggcgtcgat gacgatgacc cccttctgct ggaagttcca gccgacggcg
      901 ccgggctcgg ccaggttgcc gccgtacttg gagaggatgt ggcggagctc gcccgaggtg
      961 cggttccggt tgtcggtggt cgattcgatc atcagggcca cgccgtgggg ggcgtaggct
     1021 tcgtagacga tctcctcgta gttgacccct tccagcgtgc cggttccctt tttgatagcg
     1081 cggtcgatgt tgtcggccgg catgttgacg gccttggcgg cggcaatggc cgtccttagg
     1141 cgcgggttgc cggcgggatc accgccaccc gagcgggcgg cgatggtgat ttccttgatg
     1201 accttggtaa agctcttgcc gcgggcggcg tcgatcccgg ccttcttgcg cttgatcgtc
     1261 gcccatttcg aatgtccgga catgccttct caactctcct tttcgcgggc ggctttcgcc
     1321 tcctcgatga ttttttccgc ggaatcgcgc ggcacttcct cgtacgagga gaacttcatg
     1381 gtgaaccgcc cgcgcccctg ggtgagggag cggaggtgcg tggaatagcg gtagagctcg
     1441 gcctggggca ccagggcctt caccgactgc ccgccgggag ccgggttcat cccgaggatc
     1501 ttgccgcgct tggatgacaa atcgcccatc acgtccccca tgtggtcgtc ggggacggtg
     1561 acctcgattt cgacgatcgg ctcgagcagc accggcttgc acttttcggc cgcagcatgg
     1621 aagccgatga tgcccgccat cttgaaggcc gcttccgagg aatcgaccga gtggtgcgat
     1681 ccgtcgtaca gcgtggcctt gaagtccacc acccggtagc cggccaggat gccacgctcg
     1741 gcggcttcct ggatcccctt gtcgacggcc gggatgaact tgcccggcac gacgccgccg
     1801 accacggcat ccacgaactc gtaaccgccg ccgcgcgtgt tgggctcgag gcggatccag
     1861 gtgtcgccga actggccgcg gccgccggtc tgcttcttgt ggcgcccctg cgcctcggct
     1921 ttcccccgga gcgtttcacg gaagggaatc cggggcttcc tgagctccac gtccacgccg
     1981 tacttccgct tcaggcggtc gaccacgatg gccagctgca gctcgcccat gccgtacagg
     2041 agctgctggt gcagctcgcc gtcgaccacg gtgcgcacgg tcgggtcttc ctcgtggatc
     2101 ttgtggaggg cctgggccac cttgtcctcc tcccccttca ccttggggaa gatggcctcg
     2161 gccaggatcg gctcgggata gtcgatgccg gggagctcga gcgggtgccc tttgaccgtc
     2221 agggtgtttc cggtgtgggt gccgcggagc ttgacggcgg ccacgatgtc gccggcgccg
     2281 acggagtgca cctccttgcg ctccttcccc tgcaggagga agagcgaccc gatcttctcg
     2341 gagtggccgg cggtgacgtt gttgacctcc acgccgtgct ccagctttcc ttcgtacacc
     2401 cggatcagcg acagatcgcc gacatgcgcc tcggaaagcg tcttgaagac gagcgcgcag
     2461 aggggcttgc tcgaatcggg caccagcgtc acttcctgtc cggtggcggt gtcgatggcc
     2521 gtcccggtcg gggcttcgtg cggcgacggg aggcagacga ccagtgtgtc gagcaggcgg
     2581 cggatgccgg tgttgtgcgt ggcgctggtc atgacgacgg ggtagatccc cttctgcatg
     2641 acccccttct tcagggccgc cagctcttcc tcgatcgtga gctcctgccc ctcgaggtac
     2701 ttctcgacca gggcgtcgtc gaacgtggcc acggcttcga tgagcttttc gcgcgcttcc
     2761 tcggcttcgg gacgggcatc ggccggcacc tccacttcct tgggctgacc gtcggcgccc
     2821 atcgaatagg ccttcatcgt gaagaggtcg atgaaaccgg caaaggtggc gcccttgcca
     2881 agcggcacct gcagcggcat caccttgtcg gagaggatgg cccgcgccga atcgatcacc
     2941 ttgtgaaagt cggcatgctc cttgtcgagc atgttggcgg caatgaggac cggcagctcc
     3001 cgggcctgga gcgcttccca cgcgcgctgc gtgccgcttt ccaccccgga gagtccggag
     3061 atgaccagca ccgctccgtc cgccacgcgg atgccggcca gcatgtcccc ctggaaatcg
     3121 tcgtatcccg gcgtgtcgat caggttcagg cgggtgtcac gccattcggc gtgcccgagc
     3181 ccgaggttga tcgagatctt gcgcttcgtc tcgtccgggc tgtggtccat gacggtcgtg
     3241 ccgtcggtca ccgagccgat ccggttgatt tcgccggcgt cgaagagcat cgactcgatg
     3301 agggaggtct tgccggtgcc gccatggccg acaaaaacga tgttgcggat ctgatcggta
     3361 cggaattcct tcaccggata cctcccccgc tggggcgctg agtcgggtct cacctggaga
     3421 acggaagggc gtaaactcta tcaccggtag aatttaggtg tcaaacgagc cccggaacgc
     3481 cctcctgaaa tgcctgggcc gcggtcgaag ccgtattcga cccgtgaggc cacttagtgc
     3541 agaagtccga tgtttgctgg cccaagcttt cagcgcgcct tcagaccgct ccaagtggat
     3601 ggcagcactg
//
LOCUS       DASVOD010000039         2955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_941516, whole genome shotgun sequence.
ACCESSION   DASVOD010000039 DASVOD010000000
VERSION     DASVOD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941516"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VF720_00775"
     CDS             complement(<1..1275)
                     /locus_tag="VF720_00775"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7877907.1"
                     /translation="MFHATTRSRLLLTILTAALLVGTAFGASARERVAPWSIGKLMKG
                     NTVTPPTPVQADLSRLRWDPGNARTNSPINNPDIQVFPSADPQTEVSVSVSYTNPMRF
                     LITSNTTPTATGQGYFYSLNNGATWFGSNSFPNGNANFGDPVSLHDNLGNTFDVTLRS
                     PSGIGLISSADYGVTWGGFSNIDPLNSSGCDKEMGHVDRKVGSPFENQVYVAWTDFNL
                     TDPHVILTRSGDGYAARTDLSAALGPDLGQGVDITTGPGGQVYACWAVYAGGFLPEAG
                     IGFARSTNGGVSWTPGSQIFAITGIRSSNGPNVDFNNTRVNGFPSISCDISTGANSGR
                     IYIVYSDRSTGDSDIYLRHSDDQGSSWSAAVRVNPDPVSNGKQQWFPWIDCDPVSGDV
                     SISYFSMDTGGSGFQTKTWVSHSTDGGNTFDSF"
     gene            complement(1628..>2955)
                     /locus_tag="VF720_00780"
     CDS             complement(1628..>2955)
                     /locus_tag="VF720_00780"
                     /inference="COORDINATES: protein motif:HMM:NF037084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HEX7877908.1"
                     /translation="PMQFEMAPIFEEVNLANDHNALAMDIARKVALRHDFVCVFYEKP
                     FAGLNGSGKHCNWSMATDKGENLLEPGRTPHQNLRFLAFLAVCLKAVHDHSVALRYTI
                     ANAANDLRLGANEAPPAILSCFVGDQLTRVIERVISGTGGEDAEHFVLQMGVAKLPQL
                     ARDNTDRNRTSPFAFTGNKFEYRAVGSSANCAVPMALLNAAVADALGQMTKRLKARID
                     GGSARDEAVLGLLKEVFTETSPIRFEGNNYSDEWRHEAEKRGLPNLVDTPSAIAAFAD
                     KKHSGFLTELGIFSKEEIASRENVALERYIKQVTLEAETVIEMLETLVAPAAEKQLAL
                     TAAALKARMELKAAGVSFIAGKASESLTKRTSALGDGLTEILAAADQLKDRLKEIDGI
                     HDEAKKARRLADDVRTLMADARVAADRLETLVEDGAWVLPKYREMLFVR"
BASE COUNT          469 a          910 c         1039 g          537 t
ORIGIN      
        1 gaacgaatcg aaggtgttgc cgccgtcagt ggagtggctc acccaggtct tcgtctggaa
       61 gcccgatccg ccggtgtcca tgctgaagta gctgatcgac acgtcacccg agacgggatc
      121 gcagtcgatc cacgggaacc actgctgctt gccgttcgaa accgggtcgg gattgacgcg
      181 gacggccgcg ctccagctgg agccctgatc gtccgaatgc cggaggtaga tgtcggagtc
      241 gccggtggag cgatccgagt agacgatgta gatgcggccg ctgttggcgc cggtggagat
      301 gtcgcaggag atcgagggga agccgttcac gcgggtgtta ttgaagtcga cgttcggccc
      361 gttggacgaa cggatgcccg tgatcgcaaa gatctgcgaa cccggcgtcc aggagacgcc
      421 gccgttcgtc gagcgcgcga aaccgatgcc cgcctccggc aggaagccgc cggcgtaaac
      481 cgcccagcac gcgtacacct ggccgcccgg gccggtggtg atgtccacgc cctggccgag
      541 gtcggggccg agggccgccg aaaggtcggt gcgggccgcg taaccgtcgc cggaacgcgt
      601 gaggatcacg tggggatcgg tgaggttgaa gtcggtccag gccacatata cctggttctc
      661 gaacgggctg ccgaccttgc ggtcgacgtg acccatttcc ttgtcgcaac cggagctgtt
      721 cagcggatcg atgttgctga agccgcccca cgtcacaccg tagtcggcgc tcgagatgag
      781 tccgatgccc gaggggctgc ggagcgtcac gtcgaacgtg ttgccgaggt tgtcgtgcaa
      841 cgagacgggg tcgccgaagt tggcgttgcc attggggaag ctgttgctcc cgaaccaggt
      901 ggcgccgttg ttcagcgaat aaaaatagcc ctggccggtg gccgtgggcg tggtgttcga
      961 ggtgatcagg aaccgcatcg ggttggtgta cgacaccgac accgatacct cggtctgcgg
     1021 atcggccgag ggaaacacct ggatgtccgg gttgttgatc ggggagttgg tgcgggcgtt
     1081 gccggggtcc cagcgaaggc gactcaagtc ggcctggacc ggcgtgggcg gggtgaccgt
     1141 gttccccttc atcagcttcc cgatgctcca gggcgccacg cgttctcgcg ccgaagcgcc
     1201 gaacgccgtg cccacgagca gggcggcagt gaggatggtc agcaacaggc gtgaacgggt
     1261 agtagcgtgg aacatgggga cctccttcag cggtaggcgc tgcggtgcca cgaaaaccct
     1321 gtcttggaat cctgtcgtgc gctctgggtt ggttgtctgt tgggcggacg tagcctgagg
     1381 aaggccatgc atccggtcgg cggtgctctc ctgatgggat aaggtgggat gacgattccg
     1441 gtgggccacg ccccgcggcc ggaccgggtc atcgggttat agagccgccg aagatcagaa
     1501 cgcaagcaga aaattgtggc aggcgtgttt catgccgcac gtggagtccg gccggaacgc
     1561 aaacgggacg gagcctttcg gccccgcccc gtcgcggaac ttggttcgtg tcaatcggat
     1621 cggcgcgtca cctcacgaac agcatctccc ggtatttcgg caggacccag gcgccgtcct
     1681 cgaccaatgt ttccagccgg tcggccgcca cccgggcgtc ggccatgagg gtgcggacgt
     1741 cgtcggccag tcgccgggcc ttcttcgcct cgtcgtggat gccgtcgatc tctttgaggc
     1801 gatccttgag ctggtcggcc gccgccagga tttcggtgag gccgtcaccc agggccgaag
     1861 tgcgcttcgt caatgactcg gaggccttgc cggcgatgaa cgacacaccg gccgccttca
     1921 gctccatgcg cgccttgagg gccgccgcgg tcagcgccag ctgcttctcg gcggccgggg
     1981 ccaccagcgt ctcgagcatc tcgatgacgg tttcggcttc gagcgtcacc tgcttgatgt
     2041 agcgctccag ggccacgttc tcgcggctgg cgatctcctc cttcgagaag atgccgagct
     2101 cggtgagaaa gccggagtgc ttcttgtccg cgaaggcggc gatggccgac ggcgtgtcga
     2161 ccaggttggg caggccgcgc ttctcggcct cgtggcgcca ctcgtcgctg tagttgttcc
     2221 cctcgaagcg gatggggctc gtctcggtga agacctcttt caggagcccg agcaccgcct
     2281 cgtcccgcgc cgaaccgccg tcgatcctgg ccttcaggcg cttcgtcatc tgccccagcg
     2341 cgtcggccac ggccgcgttg aggagcgcca tcggcaccgc gcagttggcg gaggagccca
     2401 ccgcccggta ctcgaacttg ttgccggtga aggcaaacgg gctcgtccgg ttgcggtcgg
     2461 tgttgtcgcg cgcgagctgc ggcagcttgg ccacgcccat ctgcaggacg aagtgctcgg
     2521 catcctcgcc acccgtgccg gagatcacgc gctcgatgac ccgcgtgagc tggtctccca
     2581 cgaagcacga caggatggcc ggcggcgcct cgttggcgcc gaggcgcagg tcgttggccg
     2641 cgttggcgat ggtgtagcgg agcgccaccg aatgatcgtg cacggccttg aggcacacgg
     2701 ccaggaaggc caggaagcgg aggttctggt gcggcgtgcg accgggctcg aggaggttct
     2761 cccccttgtc ggtggccatg ctccagttgc agtgcttgcc gctgccgttg agccccgcga
     2821 acggcttctc gtagaagacg cagacgaagt cgtgacgcag cgccaccttg cgggcgatgt
     2881 ccatggccag cgcgttgtga tcgttggcca ggttcacttc ctcgaagatc ggcgccattt
     2941 cgaactgcat cggcg
//
LOCUS       DASVOD010000040         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_576118, whole genome shotgun sequence.
ACCESSION   DASVOD010000040 DASVOD010000000
VERSION     DASVOD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576118"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1648
                     /locus_tag="VF720_00785"
     CDS             <1..>1648
                     /locus_tag="VF720_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX7877909.1"
                     /translation="RIARRAVDRFVTPSTGPRFVAGALGPTNRTASLAIDVNHPDRRT
                     HTFEDFRAAYYDQARGLLDGGVDFLLVETVFDTLVGKAALFAVQQIFEERGASVPLML
                     SVTIVDQSGRTLSGQTVEAFWNSVSNVPLFSVGINCSLGPREMRPYIEELARVAPLPV
                     SCYPNAGLPNPLADTGFDETPATMAPHLGDWAREGFLNIVGGCCGTTPEHVKAIAEAV
                     AGVAPRAIPRIDPTLRLSGLEPLTVRPDSNFQMIGERTNVTGSPKFARLILESRYEEA
                     LAVARQQVENGANLIDINMDEGLLDSVEAMRTFLNLIGSEPDIARIPIMIDSSNWAVL
                     EAGLACLQGKGVVNSISLKDGEAEFLRRARIIRRFGAAVVIMAFDEQGQADTVDRKVA
                     ICTRAYDLLTKEVGFPPEDLIFDPNVLTVATGIEEHNDYAVAFIEATRRIKATLPGAR
                     VSGGISNISFSFRGNNPIREAMHAAFLYHAIQAGLDMGIVNAGQLAVYEEIPKDLLEH
                     VEDVLLNRRPDATERLLTFAETVKGGEKVAAREDAWRKGTV"
BASE COUNT          277 a          572 c          547 g          252 t
ORIGIN      
        1 cgcatcgccc gccgcgccgt cgaccgcttc gtgacccctt cgaccgggcc gcgcttcgtg
       61 gccggcgcgc tcggaccgac caaccggacg gcctcgctgg ccatcgacgt gaaccacccc
      121 gaccggcgga cgcacacctt cgaggacttc cgggccgcct actacgacca ggcccgcggc
      181 ctcctcgacg gcggcgtcga tttcctgctg gtcgaaacgg tcttcgacac gctcgtcggg
      241 aaggcggcgc tcttcgccgt ccagcagatc ttcgaagagc ggggcgcctc ggtgccgctc
      301 atgctgtcgg tgacgatcgt cgaccagtcg gggcgcacgc tctcgggcca gacggtggaa
      361 gcgttctgga attccgtcag caacgtgccc ctcttctcgg tggggatcaa ctgctcgctg
      421 ggaccgaggg agatgcgccc ctacatcgag gagctggccc gcgtggcgcc gctcccggtg
      481 agctgttatc ccaacgccgg tctcccgaac ccgctggccg acaccggctt cgacgagacg
      541 ccggccacca tggccccgca cctcggcgac tgggcccgcg aggggttcct caacatcgtg
      601 gggggatgct gcggcaccac gccggaacat gtgaaggcca tcgccgaggc ggtggcgggc
      661 gttgcgccgc gggccattcc ccgcatcgat cccacgctgc gcctgtcggg cctcgagccc
      721 ctgacggtgc gccccgactc gaacttccag atgatcggcg agcggaccaa cgtgaccggt
      781 tcgccgaaat tcgccaggct catcctcgag agccggtacg aggaggcgct cgccgtggcg
      841 cgccagcagg tggagaacgg cgccaacctg atcgacatca acatggacga ggggctgctc
      901 gactcggtcg aggcaatgcg gacgttcctc aacctcatcg gctccgagcc cgacatcgcc
      961 cgcatcccga tcatgatcga cagctcgaac tgggccgtgc tcgaggccgg cctggcctgc
     1021 ctgcagggga agggcgtcgt caattccatc agcctgaagg acggcgaagc cgagttcctg
     1081 cggcgtgcgc ggatcatccg ccgcttcggc gcggccgtgg tgatcatggc cttcgacgaa
     1141 caggggcagg ccgacaccgt cgatcggaag gtggccatct gcacgcgggc ctacgacctg
     1201 ctgacgaagg aagtcggatt cccgccggaa gacctgatct tcgatcccaa cgtgctgacg
     1261 gtggccaccg gcatcgagga gcacaacgac tacgcggtgg ccttcatcga ggccacccgg
     1321 cggatcaagg ccacgttgcc cggggcgcgc gtgtcgggcg gcatctcgaa tatttcgttc
     1381 tcgttccggg gaaacaatcc gatccgcgaa gcgatgcacg cggcgttcct ctatcacgcc
     1441 atccaggcgg gcctcgacat ggggatcgtc aacgccgggc agttggccgt gtacgaagag
     1501 atcccgaaag acctgctcga gcacgtggaa gacgtgctgc tgaaccggcg gcccgacgcc
     1561 accgagcgcc tcctcacctt cgccgaaacg gtgaagggtg gcgaaaaggt ggccgcccgc
     1621 gaagatgcct ggcgcaaggg aaccgtgg
//
LOCUS       DASVOD010000041         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_941605, whole genome shotgun sequence.
ACCESSION   DASVOD010000041 DASVOD010000000
VERSION     DASVOD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_941605"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF720_00790"
     CDS             <1..837
                     /locus_tag="VF720_00790"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7877910.1"
                     /translation="RLIGLDRRTNVALLSAPGLKTTPARIGESSLVTPGSRVIVVGSR
                     PVDKPLSSFGSIELDRGLIWGYSEVEMLQLNAPVYRGHVGAPVVNSEGRVVAMVCGTL
                     DGNSISQRGEGATGISGYIYEDRIIPRGGTDVSFAIPIEKAQEIAAELKEHGRVDRGF
                     MGVRVRRGEALPGTREGVSVQEVLAGGPADRVGIRVGDTILDYAGQAVRVGDDVTFLV
                     MATRPGSRVPVTVERGGQRLTLEVAIGLAPNQYPAGAEPVPAEALMGGSAEVDSPAGR
                     RP"
     gene            853..1935
                     /gene="aroB"
                     /locus_tag="VF720_00795"
     CDS             853..1935
                     /gene="aroB"
                     /locus_tag="VF720_00795"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX7877911.1"
                     /translation="MTRVRVPLPGKRYDILVGAGVRTGAARSIAAAVAARRLLIVADA
                     RAWDRHGPPLLENLRGTFDLSVVTIPPGESSKTLRRAERLFEACRDHRLGRDGAILAF
                     GGGMVGDLAGFVAATWQRGVDLVMMPTTLLAQVDSSVGGKVAVNVGGIKNLVGAFHQP
                     RLVLADTDWLATLSPRERRSGLAEVVKYGMIADRRLFERLERAGAGLMKASAEELANV
                     VVGCVRIKSAIVARDEKDEGRRLLLNYGHTIGHAFEAAAAGGLRHGEAVALGMRGAAR
                     LAEGIGWLDSASRRRQDELLDALGLPNRFSGASPRELEAKILQDKKVQDRKPRFVLTR
                     SIGSASVAPPIDASRVRRVLADLTSS"
BASE COUNT          338 a          705 c          762 g          368 t
ORIGIN      
        1 cggctgatcg ggctggatcg ccggaccaac gtggcccttc tctccgcgcc cggcctcaag
       61 acaacgccgg cgcgcatcgg cgagtcgagc ctggtgacgc ccggatcgag ggtcatcgtc
      121 gtcggcagtc ggccggtcga caagcccctc tcttcgttcg ggtccatcga gctggatcgc
      181 ggactcatct ggggctactc cgaagtggaa atgctccagc tcaacgctcc cgtctatcgc
      241 ggccacgtgg gcgccccggt cgtgaactcg gagggtcgag tcgtcgcgat ggtttgcggc
      301 acgctcgatg gcaattcgat cagccagcgg ggtgaaggcg ccacggggat ctcgggctac
      361 atctacgagg accggatcat tccgcgcggt ggcaccgacg tcagcttcgc catcccgatc
      421 gagaaagccc aggagattgc cgccgagctg aaggagcacg gccgggtgga tcgtggcttc
      481 atgggtgtcc gcgtccgccg cggtgaagcg cttccgggaa cccgggaagg cgtctccgtc
      541 caggaggtcc tcgcgggcgg cccggccgat cgggtcggca ttcgagtggg tgacacgatc
      601 ctcgactatg ccggccaggc agtgcgcgtc ggcgacgatg tcacgttcct ggtgatggcg
      661 acgaggccgg gatcgcgcgt gccggtcacc gtcgaaaggg gcgggcagcg attgacgctc
      721 gaagtggcga tcgggcttgc cccgaaccag tatcccgccg gagccgagcc ggtccccgcc
      781 gaggccctca tgggcgggtc ggccgaagtc gattctcccg ccggccgtcg gccctagcca
      841 ctaccccgcc cgatgacccg ggtccgggtg ccgctgccgg gtaaacgcta cgacatcttg
      901 gttggcgccg gtgtccgcac cggggcagcc cgctcgatcg ccgccgccgt tgccgcccgc
      961 cgattgctca tcgtggccga tgcccgggcc tgggatcggc acggtccacc gcttctcgaa
     1021 aatcttcgcg gcaccttcga tctttccgtg gtgacaattc cgcccggaga gtcctccaag
     1081 acgctccgcc gtgccgaacg cctgttcgag gcgtgccgcg atcatcgcct cggccgcgac
     1141 ggcgccatcc tggccttcgg cggcgggatg gtcggcgacc tggccggctt cgtcgccgcc
     1201 acctggcagc gcggcgtcga tctcgtgatg atgcccacca cgctcctcgc ccaggtagat
     1261 agctcggtcg gggggaaggt ggccgtcaac gtgggcggca tcaagaacct ggtcggggcc
     1321 tttcaccagc cgcgcctcgt cctcgccgac accgactggc tggcgacgct ctcgccacgc
     1381 gagcgccgtt cgggcctcgc cgaggtcgtg aagtacggca tgatcgccga tcgccgcctc
     1441 ttcgaacggt tggaacgggc aggtgcgggg ttgatgaagg cctcggcgga ggaactggcc
     1501 aacgtggtag tcggctgcgt ccgcatcaag tcggcgatcg tcgcgaggga tgagaaggac
     1561 gagggtcgtc ggctgctgct gaactacgga catacgatcg gccatgcctt cgaggcggcc
     1621 gccgcgggcg ggctccggca cggtgaggcc gtggctctcg gaatgcgggg agccgcccgg
     1681 ctggcggaag ggatcggctg gctcgattcc gccagccgcc gccgccagga cgagctcctg
     1741 gacgcactgg ggctgccgaa ccggttttcc ggggcgtcgc ctcgggagct cgaagctaag
     1801 atattgcagg acaagaaggt acaggatcga aaaccgcgct tcgtgttgac acggtctatc
     1861 ggctctgcta gcgtggcccc tccgattgat gcctcccggg tgcgccgggt actcgccgac
     1921 ttgaccagct cctaagatat cttccggcag ccagttgccg gattcgccga cgcgagatga
     1981 cgcaccgtca ctccagttca ggcggtcccc ggtgggccca cggggaattg aggactgaat
     2041 gaaactcgag gaagtccgaa aactgatcca gttggtcgaa gagagcaaca tcggcgaact
     2101 ggaggtctgg aagtggtggg gacggattcg gatttcgcgc aacgggtcat ccgggaacgg
     2161 ggtggtcgtt tcg
//
LOCUS       DASVOD010000042         1962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_211153, whole genome shotgun sequence.
ACCESSION   DASVOD010000042 DASVOD010000000
VERSION     DASVOD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211153"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VF720_00800"
     CDS             <1..374
                     /locus_tag="VF720_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006834095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX7877912.1"
                     /translation="AEGRRVAMVGDGINDAPALASADLGIAIGTGADIAVETADIALV
                     RGDVSAVAGAIHLSRRTLATIRQNLAWAFGYNLVGIPVAAGVLFPFTGLLLSPVLAGL
                     AMAVSSVSVVTNSLRLKGTRL"
     gene            606..1511
                     /locus_tag="VF720_00805"
     CDS             606..1511
                     /locus_tag="VF720_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX7877913.1"
                     /translation="MKSSSERGHRPPQFGVVAGHRPEPAPDITAAPVAVPVRTITLMS
                     GKGGVGKTTLAASLAIALGRQGRRVLLVDGDLSLSSLDLLMGVMPRHNAADLVSGRAT
                     IDDTLLESYAGVRLLPAASGDEELANLDDMRRHRLFRSLGTLGSRFDLVVIDAASGVG
                     RNVTSLARLGGENLVVTTPEPPAISAAFSLLKILDRSEAAAPPRLVVNQAEGSLEARE
                     CWGHISKACKQFLSVTPEYLGWVPLDPAVPRAVRAREPFLLSNPAGPASSAVLRLAQR
                     VLQNDPVRGPEGAGRPVGARERLAG"
     gene            1598..>1962
                     /locus_tag="VF720_00810"
     CDS             1598..>1962
                     /locus_tag="VF720_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7877914.1"
                     /translation="MATATASVTTRRRTEREAAAPPVWKKMSKEAAYERYQPLVKYVV
                     DRIAANVPKYVERDDLLNAAALGLFDALEKYDSGKGTKFETYAVWRIRGAVLDELRSM
                     DWASRSIRRKARQLDSVCR"
BASE COUNT          307 a          672 c          665 g          318 t
ORIGIN      
        1 aggcggaagg gcggcgcgtc gccatggtgg gggacggcat caacgatgcg ccggccctcg
       61 cgtcggccga cctgggcatc gccatcggca ccggcgccga catcgcggtc gagacggcgg
      121 acatcgccct ggtccgggga gacgtgtcgg ccgtggccgg cgccattcac ctctcgcgcc
      181 gcacgctggc caccatccgc caaaatctgg cctgggcgtt cggctacaac ctcgtgggca
      241 tacccgtggc cgcgggcgtg ctcttcccat tcaccggcct gctcctctcg ccggtgctcg
      301 cgggcctcgc catggcggtc tcgagcgtgt cggtggtgac gaacagcctg aggctcaagg
      361 gcacccggct ctagacgttc caaccggccc gattctggcc gctccggtcg ctttccgcca
      421 cctgccgttg catcgtgcct tgccggtggt gagggtgggc atcgccaact gatgacgtag
      481 tgccgtctcc cgtgcaatcc gccacgtggc atatgccttg cagtcgaatc gccccatcag
      541 tcgcaaacag ttcgaacgac tccgggggga ggcgttcggt caccgaaacg ggtgatgggg
      601 attacatgaa gagctcgtcc gagcgggggc atcgcccgcc gcaattcggc gtcgtggccg
      661 gacaccgtcc ggagccggcg ccggacatca cggccgcacc ggtggcggtg ccggtccgca
      721 cgatcacgct gatgtcgggc aaggggggcg tcgggaagac cacgcttgcc gcgagcctgg
      781 ccattgcgct ggggcgccag ggacggcgcg tgctgctcgt ggacggcgat ctctcgctgt
      841 cgagtctcga tctgctcatg ggcgtcatgc cgcgccacaa cgcggccgat ctcgtgtcgg
      901 ggcgcgccac gatcgacgac acgctgctcg aaagttacgc gggggtgcgc ctccttccgg
      961 ccgcttcagg cgacgaagag ctggccaacc tggatgacat gcgccggcac cggctcttcc
     1021 ggtcgctcgg caccctgggc tcccggttcg acctcgtggt catcgacgcc gcctccggcg
     1081 ttggtcgaaa cgtcaccagc ctggctcggc tgggtggtga aaatctggtc gttacgactc
     1141 ccgaaccgcc ggccatttcg gcggctttca gccttctgaa aattctcgac cggtccgagg
     1201 cggccgcccc gccgcggctg gtcgtgaacc aggccgaggg atccctcgag gcccgggaat
     1261 gctggggcca tatctccaag gcgtgcaagc agttcctgtc ggtcaccccg gagtacctgg
     1321 ggtgggtgcc actggacccg gccgtcccca gggcggtccg ggcccgggaa cctttcctcc
     1381 tgtccaaccc ggccggcccg gcctcctcgg cggtgctcag gctggcacaa cgcgtgcttc
     1441 agaacgatcc cgtccgcggt ccggaaggag ccgggcgccc tgtcggcgcc cgggaacgac
     1501 tggccggata gcggcaacgg cccgatcggg aagcaagccc ggtccggtcg aatgaatggc
     1561 gagacattca cccgaaccag gaagcgagaa gcaaacgatg gccacggcaa ccgcaagcgt
     1621 aacgacacgg cgccgcaccg aacgcgaagc ggcggcgccg cccgtctgga agaaaatgtc
     1681 caaggaagcg gcttacgagc gctatcagcc gctcgtcaag tatgtggtgg atcggatcgc
     1741 cgccaacgtg cccaagtacg tcgagcggga tgatctcctc aatgccgctg cccttggcct
     1801 ctttgacgcc ctggagaagt acgactccgg caagggcacc aagttcgaga cctacgccgt
     1861 gtggcgcatc cgcggcgcgg ttctcgacga gcttcgttcg atggactggg ccagccgctc
     1921 gatccgtcgc aaggcgcgcc agctcgattc ggtgtgccgc ga
//
LOCUS       DASVOD010000043         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_524305, whole genome shotgun sequence.
ACCESSION   DASVOD010000043 DASVOD010000000
VERSION     DASVOD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524305"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1800
                     /locus_tag="VF720_00815"
     CDS             124..1800
                     /locus_tag="VF720_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7877915.1"
                     /translation="MPRVPVYMTLDEAHRARMFPVLSEKEIARVAEHGTTRTVTAGEV
                     LVSPGQPADRLFLVLSGRLEVINTYADPESIVASYTRGMFSGDVNLLSGRPSLVQIEV
                     MEPGEVIEVPREELRALVQSDAELSEIFMKAYILRRIELRDRKLGDVVLFGSAHCRGT
                     LRIREFLDRNGHPYRYVDLDRDDSMQTTLDRFHVTEEEIPVLLHQGATMLRNPSNQEI
                     ATTLGFNTAIDEMHVRDCVIVGAGPAGLAAAVYAASEGLDVLVIETVAPGGQAGSSSR
                     IENYLGFPMGITGQELAGRAHTQAQKFGAEIMIARNAVGLSCDRKPYAITMEDGRRIP
                     ARTIIIATGAEYRKLSLDNLAQFEGAGVYYGATHTEAQVCKGEEIVIVGGGNSAGQAA
                     VYLASHARRVHLMVRGPGLQDTMSRYLIRRIETHPGIEYHTNTELTALNGNGHLESVV
                     WKCNESGEVGACDCRHVFVMAGAVPSTNWLDSCLTLDGKGFIKTGADLTPEDLTAAEW
                     PHERSPFLMETSLPGVFAVGDVRCGSVKRVASAVGEGAIAVAFVHQALKD"
     gene            1872..2147
                     /locus_tag="VF720_00820"
     CDS             1872..2147
                     /locus_tag="VF720_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX7877916.1"
                     /translation="MSADTCSHLEALDTSHLKTAERRECAECVPIGSRWVHLRTCQTC
                     GITLCCDNSPNRHATKHAKSTKHPVIASAEPGERWVYCYPDDAYGEY"
BASE COUNT          432 a          763 c          654 g          331 t
ORIGIN      
        1 aaggcggccg gtcgctcata ccgcattgtg gcggcaagat ccgcgccgcc gcaaccggcc
       61 cggcgccggg tgtagactca tcctccgttt gccgagcctc ccattttcga tcggagatcc
      121 cacgtgcccc gcgttcccgt ctacatgacc ctcgacgagg cgcaccgcgc ccggatgttt
      181 ccggtcctca gcgagaagga aatcgcccga gtggccgagc acggcacgac ccgaacggtg
      241 accgctggcg aggtgctcgt ctcccccggc cagccggccg accggctgtt cctcgtcctc
      301 tccggccggc tcgaagtcat caacacctac gccgatcccg agtccatcgt ggccagctac
      361 acgcgcggca tgttctcggg cgatgtgaac ctcctctccg gccgcccctc cctggtccag
      421 atcgaggtga tggagccggg cgaggtgatc gaagtgccgc gcgaagaact ccgcgcgctg
      481 gtccagagcg acgccgaact gagcgaaatc ttcatgaagg cgtacatcct gcgccgcatc
      541 gagcttcgcg accgcaagct cggcgacgtc gtgctgttcg gctccgccca ctgccgcggc
      601 acgcttcgta tccgcgagtt cctggatcgg aacggccacc cctaccggta cgtcgatctc
      661 gatcgcgacg acagcatgca gaccacgctc gatcgattcc atgtgaccga ggaagagatt
      721 ccggtgctgc tgcaccaggg cgccaccatg ctgcggaacc ccagcaacca ggagatcgcc
      781 accacgctcg gcttcaacac cgccatcgac gaaatgcacg tgcgcgactg cgtgatcgtg
      841 ggcgccggac ccgccggact cgcagccgcc gtgtatgcgg catccgaggg cctggatgtc
      901 ctcgtcatcg agaccgtagc gcccggaggc caggccggct cgagctcacg aatcgagaac
      961 tacctcggct tcccgatggg aatcaccggc caggagctgg ccggcagggc ccacacgcaa
     1021 gcccagaaat tcggcgccga gatcatgatc gccaggaacg ccgtcggcct ctcgtgcgac
     1081 cgcaagccct acgccatcac catggaagac ggccgccgca tccccgccag gaccatcatc
     1141 atcgccaccg gcgccgaata ccggaaactc tcactcgaca acctcgccca gttcgaagga
     1201 gccggcgtgt actacggcgc cacccacacc gaggcccagg tctgcaaagg cgaggaaatc
     1261 gtgatcgtag gaggcggcaa ctccgccggc caggcggccg tctatctggc aagccacgcc
     1321 cgccgcgtgc atctcatggt gcgcggccca ggcctccagg acaccatgtc ccgctacctg
     1381 atccgccgca tcgaaaccca ccccggcatc gagtaccaca ccaacaccga actgaccgcc
     1441 ctgaacggca acggccacct cgaaagcgtc gtgtggaagt gcaacgaatc cggcgaagtc
     1501 ggcgcctgcg actgtcgtca tgtgttcgtg atggcgggtg cggtgccatc gacgaactgg
     1561 ctcgacagtt gcctgacgct cgacggcaaa gggttcatca agaccggcgc tgacctcacg
     1621 cccgaagacc tcaccgccgc cgaatggcca catgaacggt cgccgttcct catggagacg
     1681 agccttcccg gcgtcttcgc cgtaggcgac gtgcgatgcg gcagcgtgaa acgggtggca
     1741 tcggctgtcg gcgaaggagc gattgcggtg gcgttcgtgc atcaggcgtt aaaggactga
     1801 cgacgacccc aaggacggat gcccaataag gaaggcgccg gcatcgccgg cgccaacatc
     1861 aggagacatt catgtcggca gacacctgct cccacctcga agcactcgat accagccacc
     1921 tgaagaccgc cgaacgccgt gaatgcgccg aatgtgtccc catcggttcc aggtgggtcc
     1981 acctgcgcac ctgccaaacg tgcggcatca cgctctgctg cgacaactcg ccgaatcgtc
     2041 acgcaacaaa gcatgcgaag agcacgaaac atccggtgat cgcgtcggcc gagccgggag
     2101 aacggtgggt gtactgctat ccggatgatg cgtacgggga gtattgaaac aaacggccta
     2161 gaagagcgtc agcttccgtg
//
LOCUS       DASVOD010000044         4311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_315682, whole genome shotgun sequence.
ACCESSION   DASVOD010000044 DASVOD010000000
VERSION     DASVOD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4311
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315682"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /gene="ugpC"
                     /locus_tag="VF720_00825"
     CDS             complement(<1..935)
                     /gene="ugpC"
                     /locus_tag="VF720_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX7877917.1"
                     /translation="MARVDIENVSKVYDGGVRAVDAASFTVNNEELVVLVGPSGCGKT
                     TLLRMVAGLETITSGTIRIGDRVVNEVAPKDRDIAMVFQSYALYPHMSVYDNMAFGLK
                     LRKVPKDEIDRRVRAAADTLGLLPYLDRKPRALSGGQRQRVAVGRAIVRQPQVFLFDE
                     PLSNLDAALRVQMRSEINQLHHRLSATMLYVTHDQVEAMTMADRIVVMRAGRIEQFDE
                     PLTLYRRPVNRFVAGFIGSPSMNFLKGKFQGSVFEAGGLRVEMPAAGFGPPEGPAGRE
                     LELGIRPEDLSPQRSAGTTATLRAPVALVEPMGSET"
     gene            complement(936..1826)
                     /gene="accD"
                     /locus_tag="VF720_00830"
     CDS             complement(936..1826)
                     /gene="accD"
                     /locus_tag="VF720_00830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX7877918.1"
                     /translation="MSWLKRETPGPRSQEKRDVPEGLWVKCDSCGEIIYKRELERNLH
                     VCPKCGHHFRIGSRQYATILLDGGQWEPFADDIESVDPLNFIDSKPYPDRYAAAVKKT
                     KLNDAVLTATGNLSGRPVVIAFMDFGFIGGSMGSVVGEKIARAIETARDTRSPILILS
                     SSGGARMMEGILSLMQMAKTSALLAELREMAIPFISILTNPTTGGVTASFASLGDVIL
                     AEPKALVGFAGPRVIRETVMQELPPGFQRSEFLLEHGMVDMIVGRHELKPTIGRLLDF
                     LSGTRSRSTETPAEPPESEG"
     gene            complement(1826..2302)
                     /gene="rimI"
                     /locus_tag="VF720_00835"
     CDS             complement(1826..2302)
                     /gene="rimI"
                     /locus_tag="VF720_00835"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX7877919.1"
                     /translation="MSARRSPRIAIEPMTAEWLPEVLVIERLVFGDPWSAASFRHEVE
                     ENATAVTRVARLWPDGPVLGYFVAWFIEDEVHLGNLAVHPDRQGEGTGQRLLDNLLEE
                     AGRREARMVTLEVRESNLGAQRLYLRNGFRPIAIRRRYYPDNHEDAIVMMLERTTS"
     gene            complement(2299..3084)
                     /gene="tsaB"
                     /locus_tag="VF720_00840"
     CDS             complement(2299..3084)
                     /gene="tsaB"
                     /locus_tag="VF720_00840"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX7877920.1"
                     /translation="MREGGLTLAIDSSAALGGVGLSAGEVLLGSRELGPPGQQAETVL
                     VAIDALLHDSGRELAEVGLIAVALGPGSFTGLRIGLSAAQGIGFGRDIPVIGVPTTTI
                     RMAWARLVSEDRGSSWVALLDARRSEAFLATGRSAPDDPFDLANETSPVLISLTELTH
                     RLAPSAAAATSDRPMVVCGEGVPAAAAELAALGADPAGGGAMIRLRTISPPRPPDAVR
                     LLAALGRRRFAIHGAPPLLEPLYVREPDARKPRIPVLPPGEAS"
     gene            complement(3081..3572)
                     /gene="tsaE"
                     /locus_tag="VF720_00845"
     CDS             complement(3081..3572)
                     /gene="tsaE"
                     /locus_tag="VF720_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX7877921.1"
                     /translation="MLRRVEVHSDDPDATRIVARALGDRLAEGPGGAIVLLTGPLGAG
                     KTVFVQGLAAALGVSTPVKSPTFALEHRHNGRRALHHFDLYRLSAGNDLAELGLAELM
                     AGPDVVAIEWPDRLDAVPEDAIHVSLDWPPGSDPGSDRRVILIEGPAARLDGLVIETG
                     GVS"
     gene            complement(3592..>4311)
                     /locus_tag="VF720_00850"
     CDS             complement(3592..>4311)
                     /locus_tag="VF720_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HEX7877922.1"
                     /translation="GLWPAEIAQKFPKYWQEASNDERSKNRFERELLEEQVKRLKLSG
                     PIKYSKVYHPEEANNVRRELGSYSRLPLVALVFNFLDILAHGRSDSPILQELAPDEAA
                     FRSLLYSWFTHSAIYDILRGIADQDAVVVITTDHGAVVGKRAALVYGNRDTSTNLRYK
                     YGVNLVTDEKMALVAKNPTDLRLPNEYLNKNYVFAKEDYYFVYPTNFHEYERQYRNSF
                     QHGGISMEEMILPVATLVPKR"
BASE COUNT          678 a         1444 c         1494 g          695 t
ORIGIN      
        1 gtttcgcttc ccatcggctc gaccagcgcg accggcgcgc gcaacgtggc ggtggtgccg
       61 gccgacctct ggggcgagag gtcttccggg cggatgccga gctcgagttc gcgcccggcg
      121 ggcccctccg gcgggccgaa ccccgccgcc ggcatctcga cgcgaaggcc gcccgcttcg
      181 aagacgctcc cctggaattt ccccttgagg aagttcatgg aggggctgcc gatgaaaccg
      241 gccacgaagc ggttgaccgg ccggcgatag agagtgagcg gttcgtcgaa ctgctcgatc
      301 cggccggccc gcatcaccac gatccggtcg gccatcgtca tcgcctcgac ctggtcgtgc
      361 gtgacgtaga gcatggtggc cgacaggcgg tggtggagct ggttgatctc cgatcgcatc
      421 tggacgcgca gggcggcatc gaggttcgac aggggctcgt cgaagaggaa gacctgcggc
      481 tggcgcacga tggcccttcc cacggccacg cgctgccgct ggccgcccga tagggcgcgc
      541 ggcttccgat cgagatacgg aaggaggccg agcgtgtcgg cggcggcgcg gacgcgcctg
      601 tcgatctcgt ccttcggcac cttccggagc ttgagcccga acgccatgtt gtcgtacacc
      661 gacatgtgcg gatacagggc gtacgactgg aagaccatcg cgatgtcgcg gtcctttggc
      721 gccacctcgt tcaccacccg gtcgccgatc cggatcgttc ccgaggtgat cgtctccagc
      781 cccgccacca tgcggagcag ggtggtcttg ccgcagccgg acggaccgac caggaccacc
      841 agttcctcgt tgttcacggt gaagctggcg gcatcgacgg ccctgacgcc gccatcgtat
      901 accttcgaaa cgttttcgat gtcgacgcgc gccatctacc cctcgctctc cggcggctcg
      961 gccggcgttt ccgtgctgcg gctccgcgtc cccgacagga agtcgagcag ccggccgatg
     1021 gtgggcttca gttcgtgccg gccgacgatc atgtcgacca tgccgtgctc gagcaggaac
     1081 tccgatcgct ggaaaccggg cggcagttcc tgcatgaccg tttcgcggat gacgcgcggc
     1141 ccggcaaagc cgaccagcgc cttcggctcg gccaggatca cgtcacccag cgaggcgaac
     1201 gatgccgtga cgccgccggt cgtcgggttg gtcaggatcg agatgaaggg gatggccatc
     1261 tcgcgcagtt cggccagaag ggccgaggtc ttggccatct gcatgaggga gaggattcct
     1321 tccatcatgc gcgcgcctcc cgaagacgaa aggatgagga tcgggctccg cgtgtcgcgg
     1381 gccgtctcga tggcgcgcgc gatcttctcc ccgaccaccg atcccatgct gccgccgatg
     1441 aatccgaaat ccatgaaggc gatgacgacg ggtcgcccgg agaggtttcc ggtggcggtg
     1501 aggaccgcgt cgttcagctt cgtcttcttg acggccgcgg cgtaacggtc cgggtatggc
     1561 ttggaatcga tgaagttcag gggatcgacc gactcgatgt cgtcggcgaa gggttcccac
     1621 tggccgccgt ccaggaggat ggtggcgtac tgccggcttc cgatgcggaa gtgatgtccg
     1681 cacttcgggc agacgtggag gttccgctcg agctcccgct tgtagatgat ctcgccgcag
     1741 gagtcgcact tgacccacag cccttcggga acatcgcgct tttcctgtga gcgcggcccc
     1801 ggcgtctccc gcttgagcca cgacatcagg aggtggtccg ctccagcatc atgacgatgg
     1861 catcctcgtg attgtcgggg taatagcgcc gccggatggc gatcgggcgg aatccattgc
     1921 ggaggtagag acgctgggcg cccaggttcg actcgcgcac ttcgagggtc accatccttg
     1981 cctcccgccg tcccgcttcc tcgagcaggt tgtcgaggag ccgctggcct gttccctccc
     2041 cctgccggtc cgggtgaacc gccaggttgc cgagatggac ctcgtcctcg atgaaccagg
     2101 ccacaaagta accgagaacc ggcccgtccg gccagagccg ggccacccgc gtcaccgcgg
     2161 tggcgttctc ctccacctcg tggcggaagg aggcggccga ccacgggtcg ccgaagacga
     2221 gtcgctcgat gaccagcacc tcggggagcc actcggcggt catcggctcg atggcgatgc
     2281 ggggggaacg gcgcgcactc acgacgcctc ccccggcgga agcaccggga tcctgggctt
     2341 ccgcgcgtcc ggctcgcgga cgtagagggg ttcgagcagc ggcggcgcgc catggattgc
     2401 gaagcggcgc cggcccagcg cggccagcag ccgcaccgcg tcgggtggcc gcgggggcga
     2461 gatggtgcgc aggcgaatca tggccccgcc gccagccggg tcggccccga gggcggccag
     2521 ctcggcggcc gcggcgggca ctccctcacc gcacacgacc atcgggcgat cggacgtggc
     2581 ggcggcggct gacggcgcga gccggtgcgt cagctcggtg agggatatca gcacgggaga
     2641 cgtttcgttc gccaggtcga agggatcgtc cggcgcggaa cggccggtgg ccaggaatgc
     2701 ctcgctccgg cgcgcgtcca ggagcgccac ccagctcgag ccccggtctt cggagacgag
     2761 tcgggcccag gccatccgaa tcgtggtggt cggcacaccg atcaccggga tgtcgcgccc
     2821 gaaaccgatt ccctgggcgg ccgaaaggcc gatgcgcagg ccggtgaacg agcccggccc
     2881 cagggccacg gcgatgaggc ccacctccgc aagctcacgg ccggagtcgt ggagaagcgc
     2941 gtcgatggcc acgaggacgg tttcggcctg ctgacccggc gggccgagct cacgggagcc
     3001 gagcaggacc tccccggccg agagacccac gccgcccagc gcggcggacg agtcgatggc
     3061 aagcgtgagc cccccctctc tcatgaaaca cccccggtct cgatgacgag gccgtcgagt
     3121 cgggccgccg gcccctcgat caggatcacg cgacgatcgg agccggggtc gcttccggga
     3181 ggccagtcga gcgacacgtg gatcgcgtcc tccggaacgg catcgaggcg atccggccac
     3241 tcgatggcca cgacgtcggg acccgccatc agttcggcca ggccgagttc ggcgaggtcg
     3301 ttcccagcgg acaggcgata caggtcgaag tgatggagcg cgcgtcgccc gttgtggcga
     3361 tgctcgagcg cgaaggtcgg gctcttgacc ggcgtggaca ccccgagtgc ggcggcgagc
     3421 ccctgcacga acaccgtctt ccccgcgccg agaggccccg tgaggagcac gatcgccccg
     3481 cccggcccct cggccagccg gtcaccgagc gcccgcgcca cgatgcgggt ggcatccgga
     3541 tcgtccgagt ggacctcgac gcgacgaagc accggtgcgc ccgtcatccc cctaccgctt
     3601 cggcacgagc gtggcgaccg gcaggatcat ctcctccatc gagatgccgc cgtgctggaa
     3661 cgaattccgg tattgccgct cgtactcgtg gaaattggtg ggatagacga agtaatagtc
     3721 ttccttggcg aagacgtagt tcttgttcag gtactcgttc ggcagtcgga ggtcggtggg
     3781 gttcttggcc accagcgcca tcttctcgtc ggtgacgagg ttcactccgt acttgtagcg
     3841 caggttggtg gaggtgtcgc ggttgccgta cacgagggcg gcccgcttcc ccaccacggc
     3901 gccgtggtcg gtggtgatga ccaccaccgc gtcctggtcg gcgatgccgc gcaggatgtc
     3961 gtagatggcc gagtgggtga accacgagta caggaggctg cggaaagccg cctcgtccgg
     4021 cgccagttct tgaagtatcg gcgagtcgga cctcccgtgc gccaggatgt cgaggaagtt
     4081 gaacaccagc gccaccagcg gcaggcggga atacgagccg agctcgcgcc gcacgttgtt
     4141 ggcctcctcg gggtggtaca ccttggagta cttgatcggg cccgacagct tgagccgctt
     4201 cacctgctcc tcgagcagct cacgctcgaa ccggttcttg ctgcgctcgt cgttcgacgc
     4261 ctcctgccag tatttcggaa acttctgggc gatctcggcc ggccagaggc c
//
LOCUS       DASVOD010000045         6380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_472422, whole genome shotgun sequence.
ACCESSION   DASVOD010000045 DASVOD010000000
VERSION     DASVOD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6380
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472422"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="VF720_00855"
     CDS             complement(<1..525)
                     /locus_tag="VF720_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEX7877923.1"
                     /translation="MNWRVGASTGSVIDRPIAAAVAEIQRAGIQGIEVATPPRHFDPA
                     RNLEVAALDSQLRAAGTLPVSVHAPFGGMLDLSDPNPHHRHAAIGAIVTSASALLALG
                     GRIVVVHPTDTERNGQDVGARLDHCIESLSILARALDAMGLLLAVESPLPHLIGGDAG
                     EFRHLLERVPTAGVC"
     gene            complement(522..1970)
                     /locus_tag="VF720_00860"
     CDS             complement(522..1970)
                     /locus_tag="VF720_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877924.1"
                     /translation="MTVRIRRPFAREPPVRDYYQRREVRTRIREYASLTEPGHPGCAY
                     LAAMTGLEGPYATWYDAPHYPPAALDRLLATGADIARSMLDREGLLVFLDLDFQDPDD
                     PGFAFRTPAPAFGMLEPMYAAITAELLRLDMPLLPLMTGEGYHFVGRVAWSSKAISRL
                     ASLCPETPGWLSSMAPRQPPWVHPLLGEPEARAYAGLGMVMEHLGHEIQRHASVRCPV
                     PVVMNGTVVGRAGRGRQCVSIDLTFAGDPLDMRHLRVAFGAYQRHRWRPDIFGEDLSR
                     TAGPLVVLPRVGSLEHLLAGGRGTAAAIELASRVDATIPDVALGAERLLDDYQASPVA
                     AAHRRFYAEPAMPREEWPARFDPHEWRELPPCISGSLIAPNDRLLQPAHVQHVVRAFL
                     SRGWPPRHIAALIQSRYAGDFRWGDRWTRMHAPTKTEVDVRVFSTLCFLGLDRLVDFN
                     CVSAQEKGLCPMTGCRHDLRADRHRLLERVAS"
     gene            complement(1970..2386)
                     /locus_tag="VF720_00865"
     CDS             complement(1970..2386)
                     /locus_tag="VF720_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877925.1"
                     /translation="MGRDTLDLVWDCDECGTVQSAAAGSLRVGGRDGWSPEHLVSVAA
                     EADLMARFLQLAGREELNVLGYLSAVNLGVKRGDAGGFRVVVRPCVVLAGPAEEPAAR
                     RLLDRAFGESPVCRSLKAVRLEAAFVVEPGGRSGGV"
     gene            complement(2404..2850)
                     /locus_tag="VF720_00870"
     CDS             complement(2404..2850)
                     /locus_tag="VF720_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7877926.1"
                     /translation="MKPLTLAPMTGVTNLRNEMDRLLDRFWDGSTLELPAIGERRPAM
                     DVSETNDRFQVVVEMPGMKLEDLDVRLENQVLTITGEKKEEKEETTANFHQRERIRGS
                     FVRRIQLPTAVDPKKVMADYGDGVLTVTLPKLVATEGARIAVHPQK"
     gene            3200..4165
                     /locus_tag="VF720_00875"
     CDS             3200..4165
                     /locus_tag="VF720_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7877927.1"
                     /translation="MPDTSHAWVILLAAGEGSRVSGVFRDTDGVAIPKQYWTLFGKQT
                     MLGRTIERAARLVPPSRMVPVVAARHARWWRGGLSDIPCWNFVIQPENRGTAIGILVP
                     LLRILRRDRDASVIIMPTDHDFRNEQVLGRSLARALEAVRLAPEHPVLLGMTPWASDP
                     ELGYITPGPGAEVIRTIRNFTEKPRPGLAQRLREDGALWNSFIIVANGARLRRAFQAT
                     LHGVVDRFEETSALLFGSSRILSRLYARLPIRDFSRDVLQPVAANCRVLTVPECGWTD
                     LGTRARLGAWQHEHDEGSRRLDATVTPQFLTAGQPTEAASSPCSR"
     gene            complement(4131..5066)
                     /locus_tag="VF720_00880"
     CDS             complement(4131..5066)
                     /locus_tag="VF720_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877928.1"
                     /translation="MTIRYRVLVSAIAFAGLVAGKSPVAQAAGGIGDAFITSDASDIV
                     RAYAGATGSFLGNHCASVNAIGQLGIHFGTSNGRFLVGAFGGGVDEFDAASGAYIKTY
                     NPGGGVQWAGLYVPGGNVYIGSWNTNDVREYDAVTGAFIRVVCSALGSGGGLPSDMRI
                     GPNGNLYICLYGDASVIEVDVVTGAPINQWPVPGRPNDIAFIPGQILVTAMGLNNCQR
                     FDSSSLAPLGSFAGTGWDRPHGIDIGPSDGRIYIVDGVTTQVHVFDPVTFVELNANWR
                     APDPEDKIVDIEFRRFDLPTPVRPVTWSKVKTLLQ"
     gene            complement(5323..>6380)
                     /locus_tag="VF720_00885"
     CDS             complement(5323..>6380)
                     /locus_tag="VF720_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX7877929.1"
                     /translation="PGAILVLAPSEPVRKEMLFLPVRDPDDEIWSGERPSIGGALRES
                     TGFEWIFRKTALNAQLASALRHSRNLHLLTGPTGPEGDTPPDLALYKKISGNNVDVSI
                     KNSALLLEKMRLIKDETEMAKMRRAIEITHLGLKAAIAAAKPGATEQDVARALDQAFK
                     AAGARRHAFDPIVASGINSTTLHYPVPSNRVLEAGDMLVLDVGAEFEGYAADITRTIP
                     VGGHFSPEQRKVYETVLEAQAAGFTAARPGVTLRGDVHEAARAVIRRAGWDDFFTHGI
                     GHYVGLQVHDRGDAFAPLEAGMVITVEPGVYRKDVAIGVRIEDEVLITPKGNIILSDA
                     IPRSVDAIEAWAAGQSD"
BASE COUNT         1090 a         2110 c         2150 g         1030 t
ORIGIN      
        1 acagactccg gcagtcggca cccgctcgag caggtgccgg aattcaccgg catccccacc
       61 gatgaggtgc ggcagcgggc tctccactgc caggagcaat cccatggcat cgagcgcccg
      121 cgccaggatc gacagggact cgatgcagtg atcaagacgg gctccgacgt cctgtccatt
      181 ccgttccgta tcggtcggat gcaccaccac gatccggcca ccgagcgcca ggagagccga
      241 ggccgacgtc acgatggcgc cgatggcggc gtgccggtga tgcggattgg gatcggacag
      301 gtcgagcatc ccaccgaacg gggcatgcac ggacaccgga agcgttcccg cggcacgcaa
      361 ctggctgtcg agcgccgcga cctccaggtt ccgcgcgggg tcgaaatgcc ggggaggcgt
      421 ggccacttcg atcccttgga tccccgcccg ctggatttcc gcgaccgccg ccgcgatcgg
      481 gcgatcgatc acactgccgg tggaggcccc gacgcgccag ttcatgacgc cactctctcg
      541 agcagacgat gacgatcggc ccgcaggtcg tgtcggcatc ccgtcatcgg gcagaggccc
      601 ttctcctggg cggagacgca gttgaagtcg accagccggt cgaggccgag gaaacagagt
      661 gtggagaata cccgcacgtc gacttcggtc ttcgtcggcg cgtgcatccg tgtccaccgg
      721 tccccccacc ggaaatcgcc ggcgtaacgg gactggatga gcgcggcaat gtgtcggggc
      781 ggccatccac ggctgaggaa ggcgcggacg acgtgctgca catgcgcggg ttgcaggagc
      841 cggtcgttcg gagcaatcag cgacccggat atgcagggtg gcagctccct ccattcgtgg
      901 ggatcgaacc gggccggcca ctcttcccga ggcatcgccg gctccgcgta aaagcggcga
      961 tgggcggccg ccacgggcga agcctggtag tcgtcgagga gccgttccgc cccgagtgcg
     1021 acgtctggaa tcgtggcatc cacgcgtgag gcgagttcga tggcggccgc ggtgccgcgg
     1081 ccacccgcca gcaggtgttc cagtgatccg actctcggga ggacgaccag tggaccggcc
     1141 gttcgggaca aatcctcccc gaaaatgtcc ggacgccagc gatgacgctg gtacgcgccg
     1201 aaggccacac ggaggtgccg catgtcgagg ggatcgccgg cgaacgtcag gtcgatcgac
     1261 acacattgcc ggccccgccc cgctcgaccc acgaccgttc cgttcatcac gacgggaaca
     1321 gggcaccgga cggacgcgtg ccgctggatt tcgtggccga ggtgttccat caccatgccg
     1381 agaccggcat acgcccgggc ctccggctca cccagcagcg ggtggaccca gggaggttgg
     1441 cgcggagcca tggaactcag ccagccgggt gtttccgggc agagggaggc cagccgggaa
     1501 atggccttgg aggaccatgc gacgcgcccc acgaagtggt atccctcccc ggtcatcagt
     1561 ggaaggagtg gcatgtcgag ccggagcagc tccgcggtga tcgcggcgta catcggttcc
     1621 agcatgccaa acgcaggggc cggcgtgcga aaggcgaagc cgggatcgtc cgggtcctgg
     1681 aaatcgaggt cgagaaagac gaggaggccc tcgcgatcca gcatcgaccg ggcaatgtcg
     1741 gccccggtgg cgagcagtcg gtcgagagcg gccggcgggt agtgcggagc gtcgtaccac
     1801 gtggcatatg ggccttcgag acccgtcatg gcggccaggt aggcgcagcc ggggtgcccg
     1861 ggctcggtca acgaggcata ttcgcggata cgagtccgga cctcgcggcg ctggtagtag
     1921 tcacgaaccg ggggctcgcg ggcgaacggt cggcgaatgc gtacggtcat cacacccctc
     1981 cactccggcc accgggttcc accacgaagg cagcctcgag acggacggcc ttgagcgatc
     2041 ggcacacggg agactccccg aacgcgcggt ccaggagccg acgggcagcc ggctcctccg
     2101 ccggaccggc caggaccacg cagggacgga cgacgacgcg gaagccgccg gcatcgcccc
     2161 gcttaacccc caggttgacc gccgagaggt acccgagaac gttgagctcc tcgcggccgg
     2221 ccagctggag aaaccgggcc atgaggtcgg cttccgccgc caccgatacg agatgctccg
     2281 ggctccagcc gtcgcgccct ccgacgcgca atgaaccggc agcggcggac tggacggtgc
     2341 cgcactcgtc gcaatcccac acgaggtcga gagtgtcacg gcccatggtc cgctccggcg
     2401 acgttacttc tgggggtgca cggcgatccg ggcgccctcg gtcgccacca gcttgggaag
     2461 cgtcacggtc agcacgccat ccccgtagtc cgccatgacc ttcttcgggt ccacggccgt
     2521 cggaagctgg atgcgccgca cgaagctccc gcggatccgc tcccgctggt ggaagttcgc
     2581 ggtcgtctct tccttttctt ccttcttctc gccggtgatc gtcaggacct ggttctcgag
     2641 ccgcacgtcg aggtcctcga gcttcatccc cggcatctcg accacgacct ggaacctgtc
     2701 gtttgtttcc gaaacatcca tcgcgggccg gcgctcgccg atcgccggca gttccagcgt
     2761 cgagccgtcc cagaaccgat cgagcaggcg atccatctcg tttcggaggt tggtcactcc
     2821 ggtcatggga gccagggtca gcggtttcat cgtgatttcc ttgtctcgac acccggaagc
     2881 ccgccagacg atcccgatgt cggggggatc gccgttggcg ccgacgccca cgcgtggcgc
     2941 aatccgaggg gactgcatcg gacatgccag cgccgacggg acgccagggc atcgacgatc
     3001 accggcttgg acccggcggc aacgcgaaac tgtcattttg gcagggggaa ccgccgatgg
     3061 cagtgtggcg atccggcaag tccggcggcc gggccttcag gcgggtatcg cgtggcatgc
     3121 ccgatgcaca ccagacgagg agacgctgac cccacattcg gcgtgcgtca ccgtgcaccc
     3181 atggggtgaa gggttcgaaa tgcccgatac atcacatgcc tgggtcatcc tgctcgcggc
     3241 aggggaaggt tcgcgcgttt ccggcgtctt ccgggacacg gatggagtgg ccattccgaa
     3301 gcagtattgg accctgttcg ggaaacagac gatgctcggc cggacaatcg aacgggccgc
     3361 ccgcctggtc ccgccttcac ggatggtccc cgtcgtggcc gcccggcatg cacgatggtg
     3421 gcgggggggg ctctccgaca tcccgtgctg gaacttcgtg atccagccgg agaaccgggg
     3481 aacggccatc gggattctcg tgccgctgtt gcgcattctc cggcgcgacc gcgacgcctc
     3541 ggtcatcatc atgccgaccg atcatgactt tcggaatgaa caggtcctcg gtcggtcgct
     3601 cgcgcgggcg ctcgaagccg tccgcctcgc ccctgaacac ccggtcctcc tcggcatgac
     3661 gccgtgggcg tcggacccgg agctggggta catcacgccg ggccccggcg cagaagtgat
     3721 ccggacgatc cggaatttca cggagaagcc ccgacccggc ctggcgcaac gcctgcggga
     3781 ggacggcgct ctctggaaca gtttcatcat cgttgccaac ggcgcccggt tgcgccgggc
     3841 gttccaggcg acgcttcatg gcgtcgtcga ccgcttcgag gagacttcag cgctactctt
     3901 cggatcgtcg cggatactct cccggttata cgcccggttg ccgatccgcg acttctcgcg
     3961 ggatgtgctt cagccggtcg cggccaattg ccgcgtcctg accgtgccgg agtgcggatg
     4021 gaccgacctc ggcacccgtg cgcggctcgg cgcgtggcaa catgaacatg atgaggggtc
     4081 acggcggctt gacgccaccg tgacccctca attcctgact gccggtcagc ctactgaagc
     4141 agcgtcttca ccttgctcca ggtaacgggc ctgaccggag tcgggaggtc gaagcgacgg
     4201 aattcgatgt cgacgatctt gtcctcggga tcgggggcgc gccagttcgc gttcagttcc
     4261 acgaacgtca ccggatcgaa gacgtggacc tgggtcgtga cgccgtccac gatgtagatg
     4321 cgcccatccg aaggaccgat gtcgattccg tgcggccgat cccagcccgt gccggcaaag
     4381 gagccgaggg gggccagtga cgaagagtcg aagcgctggc agttgttcag tcccatcgcc
     4441 gtgacgagga tctgacccgg aatgaaagcg atgtcgttcg gccggcccgg aacaggccac
     4501 tgattgatgg gagctccggt gaccacgtcg acctcgatga cactggcatc gccgtacagg
     4561 cagatgtaca gattcccgtt tggtccgatg cgcatatcgg acggcaatcc acccccggat
     4621 ccgagggcgg agcagacaac gcgtatgaag gcgcccgtca ctgcgtcgta ttcccggacg
     4681 tcgttcgtgt tccaggagcc gatgtagacg ttgccgcccg gaacgtagag tccggcccac
     4741 tgcacgccgc cacccgggtt gtaggtcttg atgtacgcgc cgctcgcggc gtcgaactca
     4801 tccaccccgc cgccgaacgc cccgacgagg aatcgaccgt ttgacgttcc gaagtgaatc
     4861 ccgagctggc cgatcgcatt gaccgaggcg cagtgattgc ccagaaatga cccggtggca
     4921 ccggcgtaag cgcgcacgat gtccgaggca tcgctggtga tgaaggcgtc gccgatcccc
     4981 ccggccgcct gtgccacggg cgattttccg gcgacgaggc cggcgaacgc gatggcggag
     5041 accagaactc gataccgaat cgtcatgaaa tcctcctcgt agctggacca cacggttgtg
     5101 acgcttcgtc ccgcccgcgg gatgcgcgga gaggcgaatc aatgaaggaa ggcgtgcacg
     5161 atgatcggga gtcgccagcg acgaacctca gccacgtcgg ctccaggctg atcgggcgat
     5221 caaggcaccg gcaagtttac cgtaccgacc gggtacgatc caaaggcaaa catggggccg
     5281 gaggccccgt aaccgctctt ttcttgccgt tggagcctcg gctcagtcgg actgccccgc
     5341 cgcccatgcc tcgatggcat ccaccgaacg cgggatggca tcgctcagga tgatattgcc
     5401 cttcggggtg atcaggacct catcttcgat ccgcaccccg atggccacgt ccttccgata
     5461 gacgcccggc tcgacggtga tgaccatccc ggcttcgaga ggagcaaacg cgtcgccgcg
     5521 gtcgtgcacc tgcaggccga cgtagtgccc gatgccgtgc gtgaagaagt cgtcccagcc
     5581 ggcacggcgg atgaccgcgc gggcggcttc gtgcacgtcg ccgcgaagtg tgacgccggg
     5641 gcgcgccgcc gtgaaaccgg cggcctgcgc ttcgagcacc gtttcgtaca cctttcgctg
     5701 ctcgggcgag aagtgtccgc ccaccgggat ggtgcgcgtg atgtcggcgg cgtaaccctc
     5761 gaactcggcg ccgacgtcga gcacgagcat gtcgcccgcc tcgaggacgc ggttggacgg
     5821 tacgggatag tggagcgtgg tggaattgat gccggaggcc acgatcggat cgaacgcgtg
     5881 gcgccgggcg ccggccgcct tgaacgcctg gtcgagcgcg cgggccacat cctgctcggt
     5941 ggcgcctggc ttcgcggcgg cgatcgccgc cttgaggccg agatgcgtga tctcgatggc
     6001 gcgacgcatc ttcgccatct ccgtctcgtc cttgatgagg cgcatcttct cgaggagcag
     6061 cgccgagttc ttgatcgaga cgtcgacgtt gttgccggaa atcttcttgt agagcgcgag
     6121 gtccggcggt gtgtcccctt cggggccggt cggtccggtc agcaggtgaa ggtttcgact
     6181 gtgcctcaag gccgaagcca gctgcgcgtt cagcgccgtc ttccggaaga tccactcgaa
     6241 gcccgtcgac tcgcgcagcg cgcccccgat cgaggggcgt tcgccgctcc agatttcgtc
     6301 atccggatcg cgcacgggaa ggaagagcat ctccttgcgc accggctcgg agggcgccag
     6361 gaccaggatg gcacccggct
//
LOCUS       DASVOD010000046         2984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_524495, whole genome shotgun sequence.
ACCESSION   DASVOD010000046 DASVOD010000000
VERSION     DASVOD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524495"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF720_00890"
     CDS             complement(<1..228)
                     /locus_tag="VF720_00890"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7877930.1"
                     /translation="MSGSPGVEVVRLEIPSRLELLSIIDKVVDGVADQMGFEDDDKDA
                     VAISVIEAGTNAIQHGHGHESERLVEIVFRLE"
     gene            complement(231..566)
                     /locus_tag="VF720_00895"
     CDS             complement(231..566)
                     /locus_tag="VF720_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7877931.1"
                     /translation="MTRRDDGDVTILTLSGRLMGGPDTEQFLKQIRDLIDGKRSRVIV
                     DMGDVAWVNSSGLGALIAGWTLLKEKGGSLKLIRVSRRIEQILAVTKLNTVFEIHPDE
                     ASARAGFGG"
     gene            complement(601..1569)
                     /locus_tag="VF720_00900"
     CDS             complement(601..1569)
                     /locus_tag="VF720_00900"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7877932.1"
                     /translation="MRQTVPPADAGRRLDQWLSEREPSATRSQVRRWIEDGLVTVNGA
                     SVKAGTALKTGAVIDWERPTPRNVTGGPAAESLDFGLLYEDDAIVVVDKPPGMVVHPA
                     AGAWTGTLVAALKGRGLTLATLGGPQRPGIVHRLDKDTSGCMVVARTDQAYEALTRAV
                     AARQVHRQYRALVWGDIATPSGTMEGAIARSRADRKKMTVSRKGGRPARTHWRVLARH
                     RLVTDLELTLETGRTHQIRVHCRHDGHPVFGDPTYGGRARGGSLSPSDRQRTSDWLAL
                     IDRQALHAACLEFDHPVNGERMSFTAPLPADMSRLFDRVAADAGGG"
     gene            complement(1544..2152)
                     /gene="lspA"
                     /locus_tag="VF720_00905"
     CDS             complement(1544..2152)
                     /gene="lspA"
                     /locus_tag="VF720_00905"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX7877933.1"
                     /translation="MTNDVGAPSAPAGQPTTPAAPLSRLVAFGAIALLVIALDHLTKW
                     MVVRKLPFNHDVHIAGEWVMLTHIKNTGGAFGLFPGSTLPLILVSSAASVVLCYLAVR
                     LRHDWTRLIALGLILGGAIGNLIDRIFQKKVTDFIHVGIPDGPRWPIFNVADSAVSIG
                     VGILAWYVYVKGSKADELPAPADAPAPTAEPRVDHAPDGTAG"
     gene            complement(2149..2532)
                     /locus_tag="VF720_00910"
     CDS             complement(2149..2532)
                     /locus_tag="VF720_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX7877934.1"
                     /translation="MKKKDMDNLEKRLLEERQKLLLEIKHLDETVLNQSVKDSSGDLS
                     SYSFHMADMGSDAMERERAFLTASAEGRALLEVDEALRRLYRNEYGVCEACGEDIALK
                     RLEAVPQAKLCLDCKAKEEKSGHRS"
     gene            complement(2529..>2984)
                     /locus_tag="VF720_00915"
     CDS             complement(2529..>2984)
                     /locus_tag="VF720_00915"
                     /inference="COORDINATES: protein motif:HMM:NF039585.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEX7877935.1"
                     /translation="EAAGHLAAGEPTAVVIGGHDVTVQPGWVEVLRHPAEGLALIEQG
                     GLAVAVDTRITEALTAEGRVRELVHRLQGLRKDSGLSPTDRIHVDYWADSELARALAA
                     HEELVREETLARTLSSRPEPADLAAEWDVDGLKFRVSIRPAGAAGEETR"
BASE COUNT          438 a         1018 c         1035 g          493 t
ORIGIN      
        1 ctcgagacgg aagacgattt ccaccaggcg ctccgattcg tggccgtggc cgtgctggat
       61 ggcgttggtt cccgcctcga tgaccgagat ggccacggca tccttgtcgt cgtcttcgaa
      121 acccatctgg tcggccacgc cgtcgacgac cttgtcgatg atggaaagca actcgagccg
      181 gctggggatt tcgagccgga cgacctcgac tcccggggac cccgacacga tcagcctccg
      241 aatccggccc gggccgaagc ctcgtccgga tggatctcga acacggtatt gagcttggtc
      301 acggccagga tctgctcgat ccgccgcgac acgcggatga gcttcaggct cccgcccttc
      361 tccttgagga gcgtccaccc ggcgatgagg gcgccgagcc ccgagctgtt gacccacgcc
      421 acgtcgccca tgtcgacgat gacgcgactc cgcttgccgt cgatcaggtc gcggatctgt
      481 ttcaggaact gctcggtgtc cggcccgccc atcagccggc cggacagggt gaggatggtc
      541 acgtcaccgt catctcgtcg tgtcatgcgc ctcgttccaa tcccgggaag agaacgcccc
      601 tcaaccgccg ccggcgtcgg cggccacgcg gtcgaagagg cgcgacatgt cggcggggag
      661 tggcgccgtg aagctcatcc gctccccgtt cactggatga tcgaactcca gacaggcggc
      721 gtgcaaggcc tgccggtcga tcagggccag ccagtcggag gtccgttgtc gatcggatgg
      781 cgagagcgag ccgccgcggg cccgtccgcc atacgtggga tcgccgaaga ccggatgccc
      841 atcgtgccgg cagtgtaccc ggatctggtg ggtgcgtccc gtctcgagcg tgagctcgag
      901 gtcggtgacg agccggtggc gggccaggac ccgccagtgg gtccgggccg gcctcccccc
      961 cttccggctc acggtcatct tcttgcgatc ggcgcggctc cgggcgatgg cgccctccat
     1021 ggttccgctc ggcgtggcaa tgtcgcccca gacgagggcg cgatactggc ggtggacctg
     1081 gcgggccgcc acggcgcggg tgagcgcctc gtaggcctgg tccgtacgag ccacgaccat
     1141 gcagccgctg gtgtccttgt cgagccggtg cacgatgccg gggcgttgtg gccctccgag
     1201 ggtggccagc gtgaggcccc gacccttcaa ggccgccacc agcgtgccgg tccaggcgcc
     1261 ggccgccggg tgcacgacca tgccgggagg cttgtcgacc acgacgatgg cgtcatcctc
     1321 gtagagaagg ccgaagtcca gcgattcggc ggcgggcccg ccggtgacgt tccggggtgt
     1381 gggtcgttcc cagtcgatga cggcgcctgt cttgagcgcc gtgcccgcct tgacgctggc
     1441 gccgttgacc gtgacgagtc cgtcctcgat ccaccggcga acctgggagc gggtggccga
     1501 gggctcacgc tccgacagcc actggtcgag gcggcggccg gcgtcagccg gcggtaccgt
     1561 ctggcgcatg gtccacccgc ggctcggccg tgggcgctgg cgcgtcggcc ggggcgggga
     1621 gctcgtcggc cttcgacccc ttcacgtaga cgtaccaggc caggattccc acgccgatcg
     1681 agacggccga gtcggccacg ttgaagatgg gccagcgcgg gccgtccggg atccccacgt
     1741 ggatgaagtc ggtcaccttc ttctggaaga tccggtcgat caggttgccg atggcccccc
     1801 ccaggatgag cccgagggcg atgaggcgcg tccagtcgtg gcggaggcgc acggccaggt
     1861 agcagagcac caccgaggcc gccgaggaca ccaggatcaa ggggagcgtg ctccccggga
     1921 agagcccgaa ggcgccaccg gtgttcttga tgtgcgtgag catcacccac tcgccggcga
     1981 tgtgcacgtc gtggttgaac ggcagcttcc ggaccaccat ccacttggtc aggtgatcga
     2041 gggcaatgac gaggagcgcg atggcgccga acgcaacgag gcgcgaaaga ggtgcggccg
     2101 gtgtggtggg ttgccctgcc ggcgcggaag gtgcgccgac gtcgtttgtc acgatcggtg
     2161 ccccgacttc tcttccttgg ccttgcagtc gaggcagagc ttggcctgcg gcacggcttc
     2221 gaggcgcttc agcgcgatgt cctcgccgca cgcttcgcac acgccgtatt cgttgcggta
     2281 gaggcggcgg agcgcctcat ccacttcgag gagggcgcga ccctcggccg aggcggtgag
     2341 gaaggcccgc tcgcgctcca tggcgtcgga acccatgtcg gccatgtgga agctgtagct
     2401 cgacaggtcg ccggaggaat ccttgacgct ctggttgagc acggtttcgt cgaggtgctt
     2461 gatttccagc agcagcttct ggcgttcctc gaggaggcgc ttctcgagat tgtccatgtc
     2521 cttcttcttc aacgcgtctc ctctccggct gcaccggccg gtcggatcga aacccggaac
     2581 ttgaggccgt ccacatccca ctcggcggcc aggtcggccg gctcggggcg tgacgagagc
     2641 gttcgggcca gcgtttcctc ccggaccagc tcctcgtggg cggccagggc acgtgccagt
     2701 tccgaatcgg cccagtagtc tacatgaatc cgatccgtgg gcgagagacc ggaatccttc
     2761 cgcaatccct ggagccggtg caccagctcg cgcacgcgcc cctcggccgt cagggcctcg
     2821 gtgatgcggg tatcgaccgc cacggccagc cccccctgct cgatcagggc cagcccctcg
     2881 gccggatggc gaagcacctc cacccagccc ggctggaccg tgacgtcgtg cccgccgatg
     2941 accacggcgg tgggctcccc cgccgccagg tggccggccg cctc
//
LOCUS       DASVOD010000047        13890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_420350, whole genome shotgun sequence.
ACCESSION   DASVOD010000047 DASVOD010000000
VERSION     DASVOD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420350"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VF720_00920"
     CDS             complement(<1..631)
                     /locus_tag="VF720_00920"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX7877936.1"
                     /translation="MTARIAATFLLLSTLAGGSALADVVTFTSSQDNTLYVNDGELSS
                     GAGPTMFCGRTGAFGTGARRALVQFDLSGIPAGSTVTAVQLRLVVNQTVSGTYPIALR
                     AVTASWGEGTSDAGDNGGQGTQATANDATWLHRFYNGTLWTNPGGDFSAVTSATRNVT
                     GLGAYTWGSTAQMVADVQAWVTTPASNFGYLLLGNEDATGSAKRFATREN"
     gene            complement(708..1475)
                     /locus_tag="VF720_00925"
     CDS             complement(708..1475)
                     /locus_tag="VF720_00925"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7877937.1"
                     /translation="MDFVPDAHCHLDEIDDPSNVVREAREAGVGPLLLIGMDEASSRR
                     TLDIARTLDAGLVYTAVGIHPSYVPEWDDERLEREFAYVKDTIASVDCLGEVGLDYKD
                     ARTSEQRARQQRILDRQLELAARHRKPVSFHSRRAERPSVARAVEFVRQTGLGINLHW
                     FTHSEKMARKCAEGGVYISCGPAILWREEQARVASLIDGDLLLTETDCPVPFNDESAR
                     PAWAARVAVRLAELRQEPLPVLATRLARNFRRYLGVA"
     gene            1676..3709
                     /gene="tkt"
                     /locus_tag="VF720_00930"
     CDS             1676..3709
                     /gene="tkt"
                     /locus_tag="VF720_00930"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7877938.1"
                     /translation="MSQDNDRIDLGSAETELQVNTIRTLAMDAVEKANSGHPGLPMGA
                     ADMAHVLWSRHLRFDPSDPDWPDRDRFVLSAGHGCMLQYALLHLAGYDLSLDDLKRFR
                     QWGSKTPGHPEYGHTPGVETTTGPLGQGFSNAVGMAIAESMSAARFNHGSSTIVQHWT
                     YVIASDGDMMEGVASEAASLAGHLQLGRLIVLYDDNHITIDGNTSLAFSEDVGARFEA
                     YGWHVQHVNGHDRPAVDAALTAAKVEAGRPSLIVARTHIGFGAPHKQDSEEAHGAPLG
                     PDEVKAAKKAYGWPEDAQFLVPEEVYAFWRGVAARGKQQRAEWTTRFDAWRSANPDGA
                     RLWDQSHAADLPSGWSKGAPTFPADAKGMATRAASGKMIQWLAEAVPHLVGGSADLAP
                     SNKTMIDKAAAVGPMAFAGRNFHFGVREHAMGAILSGMSLHGGLRPYGGTFLIFSDYM
                     RPTIRLACLMKRPVVYVWTHDSFYLGEDGPTHQPVEHLMALRAIPGFTLIRPCDANET
                     REAWIAALDHRGPVGLVLTRQNLPTLDRTVYGPAEGLHRGAYILACELRVDNLELILI
                     ATGSEVAIAVEAHRVLEGEGRSVRVVSLPSWELFDAQDATYRESVLPARVTRLLAVEA
                     GRSFGWEKHVGTSGRVIGLDRYGASAPDKVLAEQFGFTAANVLQVAREMLGAR"
     gene            3706..4626
                     /locus_tag="VF720_00935"
     CDS             3706..4626
                     /locus_tag="VF720_00935"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7877939.1"
                     /translation="MSRTVRAAFLLLLSLAVTVVAGCARPESKPAPTTAPQRIISCAP
                     NLTEMLFALGAGDRVIGVTRWCRYPAEATSRPPLGDLYQPNLEAMVAARPDLIVAVPG
                     NTKVLEFFAGKPEPRILETNACETIADITATLRQLGAAVGATDRAEALVRGIESGLDS
                     LRSAHANEPPISTLMILGHTEGALEQIYAVGHPTYLDELLSIVGGRNVVPVELGRYPI
                     ITREALLTMNPDLILERHLGGIATPEQKAAMIRLWDELPTLPAVRTNQVVVMDDDHMT
                     INGIDVVNTGIKLATVISKARASAPTDSTD"
     gene            4628..5425
                     /locus_tag="VF720_00940"
     CDS             4628..5425
                     /locus_tag="VF720_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7877940.1"
                     /translation="MTHEARNESLSARDISFAYHAGPPVLSGASLSIHAGSFLGIVGP
                     NGAGKSTLLHLLSGALVPSSGSVTLDGRPLAAMKRRDIARRIAVVPQSEPHGFPYRVS
                     EIVMMGRTPWLTGLLSTETAKDHVAVRKAMDAVGVSDLACRPTGQLSGGERQMVLVAR
                     ALAQEPSILLLDEPTASLDLAHQQQIFRLLTRLNAEHGLTVVVVTHDLNLAGLYCRTV
                     AVLHHGRIEVTGAPADVLRPERLESVYGATLWGTTSPAGAPIIGLSR"
     gene            5434..6435
                     /locus_tag="VF720_00945"
     CDS             5434..6435
                     /locus_tag="VF720_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX7877941.1"
                     /translation="MAAGHPGRGIVLLAILLLATLALAPLVGTTNISLSRAFDRTIPL
                     DQNIDAVIFFNTRLPRILFAALVGAALACAGTVYQALLRNDLATPYTLGISGGASFGA
                     LLAMRWLAPLVAGAAYVLTPLAALAGAGSAMLLVTLLARRPTVSDRTQTLLLAGVTLN
                     LLFASGILIVQYLSDPHQAFAMLRWMMGGLDVASVRLPAILAIPILGAFAVLMSQAQA
                     LNVMSIGDRTAIHLGLDADRVRLIGLGASSIMAALVVAWAGPIGFVGLVVPHLLRRFV
                     GPDHRFLLPAVALGGASFLIICDTLARTIAGGLELPVGIITAAIGGPFFLWVLFRRG"
     gene            6485..6613
                     /locus_tag="VF720_00950"
                     /pseudo
     CDS             6485..6613
                     /locus_tag="VF720_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973982.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(6904..7713)
                     /locus_tag="VF720_00955"
     CDS             complement(6904..7713)
                     /locus_tag="VF720_00955"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HEX7877942.1"
                     /translation="MRALPLFLFVVALAGCSGGSDRKPPAAGNAIVGTVTYREKVALP
                     PEATLVVRLEDVSRADAPAMLLAESRERANGRQVPIPFSLPYDPGRIEPGHTYALRAT
                     ISDEDELRFTTTTVTPVLSNGVTGGVELVVEPAGAEAASGGVAAGGNTASTATLENTY
                     WKAVRLYDRDIVVTDEIRETHFVLQREGHRVGGNGGCNKLFGTYRVNGDSLLFSGLGG
                     TLMACPEPGMSNERLLNEVLTKTTNYRVVGETLKLLRDGAVIGDFKAVYLE"
     gene            complement(7731..8126)
                     /locus_tag="VF720_00960"
     CDS             complement(7731..8126)
                     /locus_tag="VF720_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877943.1"
                     /translation="MSRPIRPPGHEPEFSDPRLEAGLKSLTAGTTAPGHLVAAVERKA
                     RLHPAPVVHPWAERLPALASLGLVLSMLIGALTHLPVQRALAGLTGVTPSELGRSPMV
                     VAGMVALALASLATIDWLRGAPVLRRFNR"
     gene            complement(8123..8719)
                     /locus_tag="VF720_00965"
     CDS             complement(8123..8719)
                     /locus_tag="VF720_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7877944.1"
                     /translation="MLAVDRPEAGEEPAEALREHLAAGRIEAARLALVAATKDAVYRY
                     LRHLLRNDPAVDDLFQDAYLRAFRSLEWFRGESSSTTWMLTIARNTALNRIRRLKTER
                     TWTTVTDDPPEVADAGANAERAAADLRLATDSRLLPALDRLPVAQREAVLLYYVEDQP
                     IEEVARITNRTPNTVKSDLRRARLALRAALEGPEDEGK"
     gene            8938..9336
                     /locus_tag="VF720_00970"
     CDS             8938..9336
                     /locus_tag="VF720_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877945.1"
                     /translation="MEIIIIPIVVGLFMAMLVLTNSLITSTFKRRYLHRERMAALEKG
                     VPLPDELLVETEALSKRPSNHMTAISGIIWTGFGLGILMSRLFVDADSISNDFHQFMT
                     FMAIWAFPALLVGSGLLVYAWLIRDRAPRA"
     gene            9333..12539
                     /locus_tag="VF720_00975"
     CDS             9333..12539
                     /locus_tag="VF720_00975"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7877946.1"
                     /translation="MTPGLRRVKFVRAGLLAGIVGLVALGLNGGTARAEPAPLPLGRV
                     DLGATSIVFSSSGDLFVVERSGGEARPLTTGPAWDDLPLLSPDGSQLAFARSYDGDKD
                     IYIMPMSGGEPVRLTWHPKGDFPRDWTIDGRSILFSSSREGDGVDRLYLVDAVGGNEQ
                     ALDLPRGSQASYSPDGSRLAMTFRPKFDGWRRYRGGNVPRVHVVTLASGDVEPVPAES
                     WADWQPMWINEAIWFLSERAGAGNLYRWRPGKGSPVPMTDAGRYGFESASAGPGGIVL
                     VGHGRLWMYAFDASGTKAEAGSLTPLEVRLPANVLPNLAVTTESVTDFVQQYFVSPDG
                     ATLLVQARGDIFVVPGAGGAPVNVTDSPGVADRSPGLSPDGRTLAWLSDASGEYRLNL
                     AAMAADGSLTRPAREITIGETPTFYTPPSWSPDGKYLVTADVHRRLWLVSVADGRALE
                     LDRSDYPGFDDFQPAWSPDGHRVAWVKHLPSRVRGVFMASVDGPGAGRRVTPVLQSCE
                     FPVFSADGTRLYATTAPLGPAADTFGMVGRFYRTMMARSIVSIDPDGDPESMPSTLPV
                     ELRNWVSIGRTHDGQLVGVSESPSGNPIGGRPERSAWRIDTASGKTTRLATGINAWEF
                     AAHGTAMLLLTGDTPSLASVDSAGSVPVSLDSLTARVDRRAEWRQIFHESVRFMRDWF
                     YDPGHHGQDLVALEAEYARWLPTLRTRRDLDDLVRAMFAHVSVSHLGVGGGDDKPLGI
                     PEGFQPPPPVGLAGVDVAIEGNRFRITRVFAGDGRSPLTTGPLAADAVRALPGEWLLS
                     INGQRMDASRNFHAWFEMAGRAPVNVTLGPDSSGAESRTVPIRLLRGENTLRRIDWAE
                     ANRRRVMERSGGRLGYLYLPDTGTRGVELFHQMFYAQRDREGLIIDERFNSGGAPADL
                     LIETLARRPLSAYLFRDGRDIPFPSGTMTGPMVMLINESAGSGGDTLPWMFRRAGLGP
                     LVGMRTMGAGIGLFVDIPALIDGGELDIPTRGFYNPDGAWEIENGGVVPDREVDITPA
                     DWRAGRDPQLEAAIDDALSRLERPREPTRSPRGQLPPN"
     gene            12617..13345
                     /locus_tag="VF720_00980"
     CDS             12617..13345
                     /locus_tag="VF720_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877947.1"
                     /translation="MARWLRPTARFGAIACAMLLFSFARMAPQAGAAPLVEFGEAWFY
                     DAATGPAPNPPFPAQPTIVGRVMRFGGALEPLNARLGEVEYTFVQREMSYQAADQGGI
                     PGAPGTVHQVYGGGRLEIYVDATPDANFASEASFGDGEIVLVARCDQMSFVYSSPSRT
                     YSSPIYLNAGLVLTGGTEMSRLAETPGTLRLSQVARVIMEPTALDPERTVLGYRGRLD
                     GAIELRAETPVAPTTWSGIKALVE"
     gene            complement(13349..>13890)
                     /locus_tag="VF720_00985"
     CDS             complement(13349..>13890)
                     /locus_tag="VF720_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7877948.1"
                     /translation="GSSIARGRHPAPVEWLGIFLTLGGLYWLVRPGLAAPPIVGALLM
                     ATAGMAWGFYSLIGRGSVAPLQDTAGNFARATLLAMGAGLIALALSAPVSPARPSGYL
                     LAVASGAVTSALGYVVWYAALKGLTATRAALVQTLVPVLSALGGIALLGENASWRLVG
                     AGALILAGIVLTLRPRKSA"
BASE COUNT         2140 a         4830 c         4678 g         2242 t
ORIGIN      
        1 tgttctcgcg cgtggcgaag cgcttggcgg aaccggtggc atcctcgttg ccgaggagca
       61 ggtagccgaa gttggaggcc ggcgtggtca cccaggcctg cacgtcggcc accatctggg
      121 ccgtggagcc ccaggtgtac gccccgagcc cggtcacgtt acgggtggcg ctggtcaccg
      181 ccgagaaatc gccgccgggg ttcgtccaaa gggtgccgtt gtagaagcgg tgcagccagg
      241 tggcatcgtt cgccgtggcc tgggttccct ggccgccgtt gtcgccggcg tcgctcgtcc
      301 cttcgcccca gctcgcggtg accgctcgca gcgcgatcgg atacgtaccc gaaaccgtct
      361 ggttgaccac gaggcgcaac tgcacggcgg tcaccgtgct gccggccggg atgccgctca
      421 ggtcgaactg gacaagggcc cggcgggcac ccgtgccgaa ggcaccggtc ctgccgcaga
      481 acatcgtggg gccggcgccg ctcgagagct cgccgtcgtt cacgtacagc gtgttgtcct
      541 ggctcgacgt gaacgtgacc acgtcggcca gggcgctgcc gccggccagg gtcgagagaa
      601 ggaggaaagt ggctgctatc cgtgcggtca tcggggggta cctcgcggct ggagtgatgt
      661 tcgtgggtgc ggctacgagc ggggtcgatt ttaacaccga cccggggtca ggccaccccg
      721 agatatctcc gaaaattgcg ggccagccgg gtggcgagga ccggcagggg ctcctggcgc
      781 aactcggcca gccgaaccgc cacccgggcc gcccaggccg ggcgggccga ttcgtcgttg
      841 aagggcaccg ggcagtcggt ttcggtcagc aggaggtctc cgtcgatgag cgaggcaacc
      901 cgggcctgct cctcccgcca gaggatggcc ggcccgcagg agatatagac gcccccctcg
      961 gcgcacttcc gggccatctt ctccgaatgg gtgaaccagt ggagattgat gccgagcccg
     1021 gtctggcgga cgaactccac ggcccgcgcc acgctggggc gttcggctcg acggctgtgg
     1081 aacgagaccg gtttcctgtg ccgggcggcc agctccagct gccggtcgag gatccgctgc
     1141 tggcgcgcgc gctgctcgga cgtgcgggcg tccttgtagt cgaggcctac ttccccgagg
     1201 caatcaaccg acgcgatggt gtccttcacg tacgcgaact cgcgctcgag tcgctcgtcg
     1261 tcccattcgg gaacgtagga ggggtggatg ccgacggcgg tgtaaaccag gccggcgtcg
     1321 agcgttcgcg cgatgtcgag cgtgcggcgg ctcgaggcct cgtccatgcc gatgaggaga
     1381 agagggccga cgccggcctc gcgcgcttcg cgcacgacgt tactcggatc gtcgatctcg
     1441 tcgagatggc agtgggcgtc cggcacgaag tccatcgggc catcatatca gccgcaacgt
     1501 ggtgggccga tggagggaac tccgggctcg gcaaccgcca tggctttccg ggtggctcat
     1561 caaatacaaa tagctttgtg ttataaagcc acagtgggcg gacgggtttg gaacggatag
     1621 gctcgatccc tgcgaagcgt gccggctttt cgttttcctc cgcccggagt cgaccatgtc
     1681 acaggacaac gatcggatcg atctcggctc cgccgagacc gaacttcaag tgaataccat
     1741 ccgcacgctg gccatggatg cggtggaaaa agccaattcg gggcatcccg gccttccgat
     1801 gggtgcggcc gacatggcgc acgtcctctg gtcgcggcac ctgcggttcg acccgtccga
     1861 cccggattgg cccgaccggg accggttcgt cctgtcggcc ggacacggct gcatgctgca
     1921 gtacgccctc ctccacctgg ccggctacga cctctcgctc gacgacctga aacggttccg
     1981 ccagtggggg agcaagaccc ccgggcaccc cgaatacggc cacacgccgg gcgtggaaac
     2041 gaccaccggg cctctcggcc agggtttcag caacgcggtc ggcatggcca tcgcggaatc
     2101 gatgtcggcc gcccgcttca accacggcag ctcaacgatt gtgcagcact ggacgtacgt
     2161 gatcgcctcc gacggcgaca tgatggaagg ggtggcttcc gaggccgcgt cgctggccgg
     2221 ccacctgcag ctcggccggc taatcgttct atacgacgac aaccacatca cgatcgacgg
     2281 caacacctcg ctggccttct cggaagacgt cggcgcgcgc ttcgaggcct acggctggca
     2341 tgtgcagcac gtgaatggcc atgatcgccc ggcggtggat gcggccctca cggccgcgaa
     2401 agtggaggcg ggccgcccca gcctcatcgt ggcgcgcacg cacatcggat tcggcgcgcc
     2461 gcacaagcag gacagcgagg aggcgcacgg cgcgcccctg ggccccgatg aggtgaaggc
     2521 ggccaagaag gcctacggtt ggccggaaga cgcccagttc ctcgtaccgg aagaggtgta
     2581 cgccttctgg cgcggcgtgg ccgcgcgcgg caagcaacag cgcgccgagt ggaccacccg
     2641 tttcgacgcg tggcggagcg ccaatcccga tggcgcgcgc ctctgggacc agtcccacgc
     2701 cgccgacctg ccaagcggct ggtcgaaggg cgcccccacc ttcccggccg acgccaaggg
     2761 aatggccacg cgggccgcct cgggcaagat gatccagtgg ctggccgaag cggtgcccca
     2821 cctggtgggc ggctcggccg acctggctcc ttccaacaag acgatgatcg acaaggctgc
     2881 cgcggtcggc ccgatggcgt tcgcagggcg caacttccac ttcggcgtgc gcgaacacgc
     2941 catgggggcc atcctcagtg gaatgagcct tcacggcggg ctccgcccgt acggcgggac
     3001 gttcctcatc ttcagcgact acatgcgtcc cacgatccgg ctggcctgcc tcatgaagcg
     3061 gcccgtggtg tacgtctgga cgcacgactc gttctatctc ggcgaggacg gcccgacgca
     3121 ccagccagtc gagcacctga tggcgctccg cgcgattccc ggtttcacgc tcatccggcc
     3181 gtgcgatgcc aacgaaacgc gcgaagcctg gattgccgcg ctcgaccaca gaggccccgt
     3241 tgggctggtg ctcacgcgcc agaatcttcc gacgctcgac cgcaccgtgt acgggccggc
     3301 cgaagggctg caccggggcg cctacatcct ggcctgcgag ttacgcgtcg acaatctcga
     3361 gcttattctc atcgcgaccg gctcggaggt ggccatcgcg gtggaggcgc atcgggtcct
     3421 cgaaggtgaa ggacgatcgg ttcgcgtcgt cagcctgccg tcgtgggaac tgttcgacgc
     3481 ccaggacgcg acctatcgcg agagcgtcct tccggctcgc gtcacccgcc tactcgccgt
     3541 ggaagccggt cgcagcttcg gctgggaaaa gcacgtgggg acgtccggac gcgtgatcgg
     3601 cctcgaccgt tacggcgcct ccgcgcccga caaggtgctg gccgaacagt tcggattcac
     3661 cgccgccaac gtgttgcagg tggcccgcga gatgctcggg gccagatgag tcgaacggtc
     3721 cgggccgcgt tcctccttct gctgtcgctc gccgtcaccg tcgttgccgg ctgcgcacgt
     3781 cccgaatcga aaccggcgcc caccaccgcc ccacagcgga tcatctcgtg cgcaccgaac
     3841 ctcaccgaaa tgctcttcgc gctcggcgcc ggcgaccgcg tcatcggcgt gacccgctgg
     3901 tgccgctacc ccgccgaggc caccagtcgc ccgccgctcg gcgacctgta ccagccgaac
     3961 ctcgaggcca tggtggccgc ccgccccgat ctcatcgtcg cggttcccgg caacacgaag
     4021 gtgctcgaat tcttcgccgg caagcccgag ccacgcatcc tcgaaacgaa cgcctgcgag
     4081 acgatcgccg acatcacggc caccctccgc cagctgggcg cggccgtcgg agccaccgac
     4141 cgggccgagg cgctggtccg cggcatcgag tcgggcctcg attcgctgcg gtcggcacac
     4201 gcgaacgagc cgcccatctc aacgctcatg atcctcggcc acaccgaggg agcgctcgag
     4261 cagatctacg cggtcggcca tccaacgtat ctggatgaac tcctctcgat cgtcggcgga
     4321 cggaacgtgg tgcccgtcga attggggcga tatccgatca tcacgcgcga agcgctcctc
     4381 accatgaacc ccgacctcat cctcgagcgc cacctggggg gcatcgccac gccggagcag
     4441 aaggcggcca tgatccgcct gtgggacgaa ttgccgacgc tccccgcggt tcgcacgaat
     4501 caggtggtcg tgatggacga cgaccacatg acgatcaacg gcattgatgt cgtgaacacc
     4561 ggcatcaaat tggcgacggt gatatcgaag gcgcgcgcga gcgcgccaac cgattcgacc
     4621 gactgacatg acacacgaag cacgcaacga gtcgctcagc gcccgggaca tttcgtttgc
     4681 ctaccacgcc ggccctcccg tgctgtcggg cgcgtccctt tcgatccacg ccggatcgtt
     4741 tctcggcatc gtcggcccca acggagcggg caagagcacg ctgctgcatc tgctgtccgg
     4801 cgcgctggta ccctcctccg ggtctgtgac gctcgacggc cgcccgctcg ccgccatgaa
     4861 gcgccgcgac atcgcccgtc gcatcgccgt ggtgccgcag tccgaaccgc acggctttcc
     4921 ctaccgcgtc agcgagatcg tgatgatggg acggacaccc tggctgaccg gtctcctttc
     4981 cacggagacg gccaaggatc atgtcgcggt ccgcaaggcc atggacgccg tcggggtttc
     5041 cgacctggcc tgtcgcccca ccggacagct ttccggcggc gaacgccaga tggtgctggt
     5101 ggcgcgggca ctggcacagg agccgtccat cctcttgctg gatgaaccga cagcctcgct
     5161 cgacctggcc caccagcagc agatcttccg gttgctcacg cggctgaacg cggagcatgg
     5221 gctcaccgtg gtggtcgtca cgcacgacct gaacctggcc ggcctgtatt gccgaacggt
     5281 ggccgtcctg catcacgggc gcatcgaagt gacgggcgcg ccggccgacg tcctccggcc
     5341 cgagcgcctc gagtcggtgt acggcgccac gctgtggggc accacatcgc cggccggagc
     5401 gccgatcatc gggctcagtc ggtgaagggc aacgtggccg cgggacatcc ggggcgtggg
     5461 atcgtgctgc tggcgatact gctgctggcc acgctggccc tggctccact ggtgggcacc
     5521 acgaatatct cgctctcgcg ggcctttgat cggaccatcc cgctcgacca gaacatcgac
     5581 gcggtcatct ttttcaacac gcgtcttccc cggatcctgt tcgctgcact ggtcggggcc
     5641 gcgctggcct gcgccggcac ggtctaccag gctcttctcc gtaacgacct ggcgacgccc
     5701 tacacgcttg gtatatcggg cggagcctcg ttcggtgcgc ttctcgccat gcgatggctg
     5761 gcacccctcg tggcaggagc cgcctacgtc ctgacaccgc tggcagccct ggccggcgcg
     5821 gggtcggcca tgctgctggt taccctgctg gcccggcgcc cgacggtgtc cgaccgcacg
     5881 cagacgctgc tgctggccgg cgtcacgctg aacctgttgt tcgcgtcggg aatcctgatc
     5941 gtccagtacc tctcggaccc ccaccaggct ttcgccatgc ttcggtggat gatgggagga
     6001 ctcgacgtgg cttcggtcag gctgcccgcc atcctggcca tccccattct tggcgcattc
     6061 gccgttctga tgtcacaggc ccaggcgctg aacgtgatgt cgatcgggga tcgcactgca
     6121 atccacctcg gactcgatgc ggatcgggtc cggcttatcg gcctcggagc gtcatcgatc
     6181 atggcggcgc tcgtggtggc ctgggcgggc ccgatcggtt tcgttggcct ggtcgtacct
     6241 catcttcttc gccgcttcgt gggtccggac catcggttcc tcctcccggc cgtcgcgctg
     6301 ggtggggcct cattcctgat catctgtgac acgctcgcca ggaccattgc cgggggtctc
     6361 gagctccctg tcggaattat cacggccgcc atcggcggcc ccttcttcct ctgggttcta
     6421 ttccggcgcg gataaggccc tttttttctt tgataaacat tcaaccaaat ggttgaattt
     6481 atccatgccg acccccgccc gcttcgatcg cgcctttgcc gccctggccc atccggcccg
     6541 ccggcgcatc gtggagcggc tgacccgcgg gccggcccgg gtgacggagc tggcggcgcc
     6601 gttcgacatg tcgctgacgc acacggggct gccgagcgag gactcggcca atcagcacca
     6661 gggcggttgg tcggaattca tggatatcct ggcgacgagg gtctgaccgg cggcggcccc
     6721 gggtcggctg cgtcgactgt gccccaaggt gacgacaatg tggggccgca aggtcagcat
     6781 ctccgagcgt tgtcttacat tgcaagacac gtcggaattc gttcatcaca acggccgccc
     6841 gttaagcccg aattcccgcc gcaacgcacc gcgccgagcc tgcgagcaaa ccagcgagca
     6901 cccctactcc aggtacaccg ccttgaagtc cccgatgacc gcaccgtcgc ggaggagctt
     6961 cagcgtctcg ccgacaacgc ggtagttggt cgtcttcgtg agcacctcgt tcagcagccg
     7021 ctcgttggac attcccggtt cggggcacgc catgagcgtg ccgccaagcc ccgagaagag
     7081 gagcgaatcg ccgttcacgc ggtaggtgcc aaagagcttg ttgcagccgc cgtttcctcc
     7141 cacccggtgc ccttcccgct gcagcacgaa gtgggtttcg cggatctcgt cggtgaccac
     7201 gatgtcgcga tcgtagaggc gcaccgcctt ccaatacgtg ttctcgagcg tggcggtcga
     7261 ggccgtgttg ccgccggcgg caacgccgcc cgaggcggcc tcggcgccgg ccggttccac
     7321 gaccagctcc actccccccg tgaccccgtt ggagaggacc ggcgtcacgg tcgtcgtcgt
     7381 gaaacgaagc tcgtcctcgt cgctgatcgt ggcccgcagg gcgtaggtat gcccgggctc
     7441 aatccgaccg ggatcgtagg gaagggagaa tggaatcggg acctgacggc cgttggcgcg
     7501 ctcgcgcgat tcggccagga gcatggccgg ggcatcggcg cgggaaacat cctcgaggcg
     7561 cacgaccagg gtggcctccg ggggaagcgc gaccttctcg cgataggtga cggtgccgac
     7621 gatggcgttg ccggcggcgg gcggcttcct gtcggagccg ccggaacatc cggccagggc
     7681 aacaacgaac agaaacaggg gcagggcgcg catcgacggc tcctcccgga tcagcgattg
     7741 aaacggcgga gcaccggcgc cccgcgcaac caatcgatgg tagcaagcga ggcgagcgcc
     7801 agtgccacca ttccggccac gaccatcgga gagcggccga gttcgctggg tgtgaccccc
     7861 gtcagtccgg caagcgcccg ctgcaccggc aggtgggtca atgcgccgat cagcatcgag
     7921 agcaccagcc ccagcgacgc cagtgccggc agtcgctcgg cccaggggtg cacgacgggc
     7981 gccgggtgga gccgggcctt ccgttcgacc gccgccacca ggtggccggg cgcggtcgtc
     8041 ccggccgtca gcgacttgag cccggcttcg agccggggat ccgagaattc gggttcgtgc
     8101 ccgggggggc gtatcggtcg gctcatttcc cctcgtcctc cggaccttcc agggcggcgc
     8161 gcagggccag gcgggcgcgc ctcaagtcgc tcttcacggt gttgggcgtc cggttcgtga
     8221 tccgggccac ctcctcgatc ggctggtcct ctacatagta aaggaggacg gcctcccgtt
     8281 gggccaccgg cagtcgatcc agcgccggca gcaaccggct gtcggtggcg aggcgcaggt
     8341 ccgcggccgc gcgttcggcg tttgcgcccg catcggccac ctcgggcggg tcgtcggtga
     8401 cggtggtcca ggtccgttcc gtcttgaggc gccggatccg gttcagggcc gtgttgcggg
     8461 cgatcgtcag catccaggtc gtcgagctcg attcgccgcg aaaccactcg agcgaccgga
     8521 acgccctgag atacgcatcc tggaagagat catccacggc agggtcattc ctcagcagat
     8581 ggcgcaggta gcgatagacc gcgtccttcg tggcggccac cagcgccaac cgggcggcct
     8641 cgatccggcc ggccgccagg tgttcgcgga gcgcctcggc cggctcctcg ccggcctctg
     8701 gcctgtcgac tgccaacatc cccctcctcc gagggcgacc ggcgcccgga tgcccggaca
     8761 cgcggctccc gcggatggtt gcagccggga ggcgggtgct ccggcaccga cccggcgggg
     8821 tgcaaccggc cggctacgcc ccgtgtctcc cccgttcgaa cgcgaccgga cggaacggcg
     8881 agtcgcgccg tgaccgccac gctaacagag cccgtaccgg gcgtggagat ccccgcaatg
     8941 gaaatcatca tcatcccgat cgtggtgggc ctgttcatgg cgatgctcgt gctgaccaat
     9001 tcgttgatca cgtccacgtt caagcgtcgc tacctgcatc gtgaacggat ggcggccctc
     9061 gaaaagggcg ttcccctgcc ggacgaactg ctcgtggaaa ccgaggccct gagcaagcgc
     9121 cccagcaacc acatgaccgc catcagcggc atcatctgga ccggcttcgg cctcggcatc
     9181 ctgatgtcgc gcctgttcgt cgacgcggat tcgatcagca acgacttcca ccagttcatg
     9241 accttcatgg ccatctgggc cttcccggcc ctgctcgtgg gctcggggct cctggtctac
     9301 gcgtggctca tccgcgatcg agcgccgcgg gcatgacgcc ggggcttcga agggtcaagt
     9361 tcgttcgcgc cgggctcctc gccggaatcg tcggcctcgt ggccctcgga ctgaatggcg
     9421 ggacggcccg ggccgagccc gctcccctcc cgctgggtcg ggtcgacctt ggcgcgacgt
     9481 cgatcgtgtt cagttcctcc ggcgacctct ttgtcgtgga acgctcggga ggcgaggcgc
     9541 gaccgctgac gacaggaccc gcctgggacg acctgccgct cctctctccc gacggctcgc
     9601 aactggcctt tgcgcggtcg tacgacggcg acaaggacat ctacatcatg ccgatgtcag
     9661 gcggtgagcc ggtgcggctc acctggcatc ccaaggggga cttcccgcgc gactggacga
     9721 tcgacgggcg atcgatcctc ttttcatcga gccgggaagg ggatggggtc gaccgcctct
     9781 acctggtgga cgccgtgggc ggcaacgagc aggcgcttga tcttccgcgg ggatcgcaag
     9841 cctcgtacag cccggacgga tcgcggctcg ccatgacatt ccggccgaag ttcgacggct
     9901 ggcgacgcta ccgcggcggc aacgtgccgc gcgtgcacgt ggtgacgctg gcgtcgggag
     9961 atgtcgagcc ggtaccggcg gaaagctggg ctgactggca gccgatgtgg attaacgagg
    10021 cgatctggtt cctctccgag cgggccggcg cgggaaacct gtatcgctgg aggccgggca
    10081 agggttcccc ggtcccgatg accgatgccg ggaggtatgg tttcgagtcg gcttccgcgg
    10141 gtcccggcgg aatcgtgctg gtcgggcatg ggcggctctg gatgtacgcg ttcgacgcgt
    10201 cgggcacgaa ggccgaggcc ggctcgctca ccccgctcga ggtgcgcctg ccagccaacg
    10261 tgctaccgaa cctcgccgtc accaccgagt ccgtcaccga tttcgtgcag cagtatttcg
    10321 tgtcgccgga tggagcgacg ctcctcgtgc aggcacgcgg cgacatcttt gtcgtcccgg
    10381 gcgccggcgg cgcgccggtc aacgtcaccg actcgcccgg cgtggccgat cgatcgcccg
    10441 gcctctcacc ggacggacgc acgctcgcct ggttgtcgga tgccagcggc gagtatcgat
    10501 tgaacctggc cgcaatggcg gccgacggct cgctcacgcg ccccgcccgg gagatcacca
    10561 tcggcgagac gccgaccttc tacacgccgc cgtcgtggtc gccggacgga aagtacctcg
    10621 tcacggccga cgtgcatcgc cggctctggc tggtgagcgt ggcggatggg cgtgcccttg
    10681 aactcgatcg ctccgactat ccaggcttcg acgacttcca gccggcgtgg agccccgacg
    10741 gccaccgggt ggcctgggtg aagcacctgc cgagccgcgt gcggggcgtc ttcatggcgt
    10801 cggtcgacgg gccgggcgca gggcgacgcg tgacaccagt gctccagtcg tgcgaattcc
    10861 cggtcttcag cgcggacgga acgcggttgt acgccacgac ggcgcccctc ggcccggcgg
    10921 ccgacacgtt cggcatggtc gggcggttct atcgcacgat gatggcgcgg tccatcgtct
    10981 cgatcgaccc ggatggcgat cccgaaagca tgcccagcac gctcccggtc gaattgcgca
    11041 actgggtgtc gatcggccgg acgcatgacg gccagctcgt cggcgtgtcg gagtcgccat
    11101 ccggcaaccc gatcgggggg cggccggaac ggagcgcctg gcggatcgat acggcctccg
    11161 gaaagaccac ccgtctcgcc accggcatca acgcctggga gttcgcggcc cacggcacgg
    11221 cgatgctcct gctcacgggc gacacgccgt ccctcgcgtc ggtcgattcc gccgggtcgg
    11281 taccggtatc gctcgacagc ctcaccgcgc gcgtggatcg gcgggcggag tggcggcaga
    11341 tcttccacga gagcgtccgg ttcatgcgcg actggttcta cgacccgggt caccacggcc
    11401 aggacctggt cgcgctcgag gccgagtacg cccgctggct cccgacgctc cgcacgcggc
    11461 gcgacctcga cgacctcgtc cgcgcgatgt tcgcgcacgt gtcggtgagt cacctcggcg
    11521 tgggcggcgg ggatgacaag ccgctcggga tcccggaggg gttccagccg ccgccgcccg
    11581 tggggctcgc gggcgtggat gtcgcgatcg aggggaaccg cttccggatc acgcgcgtgt
    11641 tcgcgggtga cggacgctct ccactcacca cgggccccct ggccgccgac gcggtgcgcg
    11701 ccttgccggg cgagtggttg ctctcgatca acggtcaacg gatggatgcc tcgaggaatt
    11761 tccatgcctg gttcgagatg gcggggcgcg cacccgtgaa cgtgacgctc gggcccgact
    11821 cgagcggagc cgaatcgcga acggttccga tccgcctcct gcgcggcgag aacacgcttc
    11881 ggcggatcga ctgggccgaa gccaaccgcc gtcgggtaat ggaacgctcg ggagggcgcc
    11941 tcggctacct ctaccttccc gacaccggca cgcgcggggt ggaactgttc caccagatgt
    12001 tctatgccca gcgcgaccgt gagggtctca tcatcgacga gcgcttcaac tcgggcggcg
    12061 cgccggccga cctcttgatc gaaacgctgg cccggcgccc gctcagcgcc tacctcttcc
    12121 gcgacggccg cgacatcccc ttcccatcag gcacgatgac cgggccgatg gtgatgctca
    12181 tcaacgagtc ggccggctcg ggtggcgaca cgcttccctg gatgttccgc cgcgccgggc
    12241 tcggacccct ggtggggatg cggacgatgg gggccgggat cggcctgttc gtcgacatcc
    12301 cggccctgat cgacggcggg gagctcgaca tcccgacccg gggcttctac aacccggacg
    12361 gcgcctggga gatcgagaat gggggcgtgg tcccggaccg ggaggtggac atcacccccg
    12421 ccgactggcg ggccggacgc gatccgcagc tcgaggcggc catcgacgac gcactctcga
    12481 ggctggaacg cccgcgggag ccgacgcgga gccccagggg ccagctgccg ccgaactagt
    12541 cggagcggag gagcttgtcg gctatactgg gggggcttcc gatccccggt cccgttccct
    12601 cttccggagg cttctcatgg cccgctggct ccgacccacc gcacgtttcg gcgcgatcgc
    12661 ctgcgccatg cttcttttct cgtttgcccg gatggcgccc caggccggtg ccgcgccgct
    12721 ggtggaattc ggcgaagcgt ggttctacga cgccgccacc ggccccgcgc ccaacccgcc
    12781 gtttcccgcg caaccgacga tcgtcggacg ggtgatgcga ttcggcggtg cgctcgagcc
    12841 gctcaatgcc cggctcggag aggtcgagta cacgttcgtg cagcgcgaga tgtcgtacca
    12901 ggcagccgat caggggggga tccccggcgc gcccggcacg gtccaccagg tttacggagg
    12961 cgggcgactc gagatttacg tcgatgccac ccccgatgcc aacttcgcga gcgaggcatc
    13021 attcggtgat ggcgagatcg tgctggtggc ccgctgcgac cagatgtcgt tcgtctactc
    13081 gtcgccgtca cgcacctatt cgagcccgat ctacctgaac gccggcctgg tcctgacggg
    13141 cggcaccgaa atgagccggc tcgcggaaac ccccggcaca cttcgcctct cgcaggtggc
    13201 gcgcgtgatc atggagccga ccgccctcga tccggaacgg accgtgcttg gttatcgggg
    13261 tcgactcgac ggggccatcg aactgcgcgc cgagacaccc gtcgccccga caacctggag
    13321 cggcatcaag gcgctggtgg agtaaccctc aggccgactt ccgtggccgg agcgtgagga
    13381 cgatgccggc caggatgagc gccccggccc cgaccagccg ccatgaggca ttctcgccga
    13441 gcagggcgat ccctcccagt gcggacagga ccggcaccag cgtctgcacg agcgcggccc
    13501 gcgtggcggt cagccccttc agcgcggcat accagaccac gtaacccaac gccgaagtga
    13561 cggcacccga agcgacggcg aggagatagc ccgagggcct ggccggcgac accggcgcgg
    13621 agagcgccag cgcgatgagt cccgccccca tcgccagcag ggtggcccgc gcgaaattcc
    13681 cggccgtgtc ctgaagcggc gccaccgaac cccggccgat cagcgagtag aagccccagg
    13741 ccatgcccgc cgtggccatg agcagcgccc ccacgatcgg cggcgccgcc aggccgggcc
    13801 gcacgagcca gtagaggccg ccgagcgtca ggaagatgcc cagccactcg accggcgccg
    13861 ggtgacgccc ccgtgcgatg gacgatccga
//
LOCUS       DASVOD010000048         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_889670, whole genome shotgun sequence.
ACCESSION   DASVOD010000048 DASVOD010000000
VERSION     DASVOD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889670"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VF720_00990"
     CDS             <1..390
                     /locus_tag="VF720_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7877949.1"
                     /translation="RDKPRLDTPELQNDFETAFRYTFAARLGQRNYPEAIRLTHKLVT
                     ERLGFAPAYRVLGYALFNIDRATDALAAYQRAVSLDPDYGEAHYALALMFAMGDKAAG
                     REHFRRAMELGIKDERQLEQQFYPSTQ"
     gene            429..1736
                     /locus_tag="VF720_00995"
     CDS             429..1736
                     /locus_tag="VF720_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX7877950.1"
                     /translation="MTMTRDDRHTPAPLRLAGVAALFFSLFLTAAAAGQSTLNPIPAT
                     PDTSGAAQAPLPPLPAAPRPTIMIDLDRNLPRLHSVAAVEALVDTIARTGFGRIIVDV
                     KLRDGRVLFPSRVAPPLDAGFDYFEAFRKAADRHDIDVIAYASVFAEGDPSTGKGLAY
                     EHPEWQQMLMIEGRGVLRQAESPEAGQFILLNPLLPSVQKYEATSISDMIQNLKPDAI
                     LLNETRFISRESDLSDSSRVSFDAWTGLSPLDWPKDILDKEHPRFPLWQAFRVGVIHE
                     FVNRIKTLRDGVAPGMPIILSAPAYWEPATTLGVNWAHSTFRPNLWYATDRFRARGLA
                     DIVDELALVSRDANPAAIREIMIGVRRATRHQRPVGLILLVEQFQNRPGRLLEAVQTL
                     NNGGFGVTVSDAGQIGAYGMWDVLAEAIRPNPPKPDSPDGGGR"
     gene            1736..>2117
                     /locus_tag="VF720_01000"
     CDS             1736..>2117
                     /locus_tag="VF720_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877951.1"
                     /translation="MRPVLVRTLLAFPMLLVACSSGDKTLTEADGGVAPADPSYETVF
                     TILDRNCAACHHDSEDAGAGPRFLEDDRDSSTCAGIERGLDDILDSAVDNERMPPGAW
                     PRLTEEEKLVIRLWVEEGACSPCRP"
BASE COUNT          350 a          754 c          676 g          337 t
ORIGIN      
        1 cgggacaagc cccggctcga cacaccggag ttgcagaacg acttcgagac ggcgttccgc
       61 tacacctttg cggcccggct cgggcagcgc aattacccgg aagccatccg gctgacccac
      121 aagctcgtga ccgagcgcct cggtttcgct ccggcctacc gcgtgctggg ttacgctctc
      181 ttcaacatcg accgggccac cgatgccctg gccgcctacc agcgggccgt gagcctcgac
      241 ccggattacg gcgaggccca ttacgccctg gccctcatgt tcgccatggg ggacaaggcg
      301 gccggtcgcg agcatttccg ccgggcgatg gaactcggca tcaaggacga acgccaactg
      361 gagcagcagt tctacccgtc cacgcagtag ccgggcgtgc gccggctggc aaggaatcac
      421 gaacaacgat gacgatgacg agagacgacc gacatactcc cgccccgctg cggctggccg
      481 gggtggccgc cctcttcttc tcgctcttcc tcacggcagc cgccgccggg cagagcacgc
      541 tgaatccgat ccccgccacc ccggacacct ccggcgcggc ccaggcgcca ctgccaccgc
      601 tgccggcggc gccccggccc acgatcatga tcgacctcga tcgcaacctg ccccgcctcc
      661 actcggtggc cgcggtggag gccctggtcg atacgatcgc ccggacggga ttcggccgca
      721 tcatcgtgga cgtgaagctg cgcgatggcc gcgtgctgtt cccgagccgg gtggcgcccc
      781 ccctcgatgc gggcttcgac tacttcgagg ccttccggaa ggcggccgac cgccacgaca
      841 tcgacgtcat cgcctacgcc tcggtcttcg ccgagggaga tccttcgacc ggcaaggggc
      901 tggcctacga acaccccgag tggcagcaga tgctgatgat cgaagggcgc ggtgtgttgc
      961 gccaggccga atccccggaa gcggggcagt tcatcctgct gaacccactc ctgccgtcgg
     1021 tgcagaagta cgaggccacg tcgatctccg acatgatcca gaacttgaag cccgatgcca
     1081 tcctcctcaa cgaaacgcgc ttcatctcgc gcgagtcgga cctctccgat tcgagccggg
     1141 tgagcttcga tgcctggacg ggactctcgc cgctcgactg gccgaaggac atcctcgaca
     1201 aggagcatcc acgcttcccg ttgtggcagg ccttccgtgt gggggtcatc cacgagttcg
     1261 tgaaccggat caagacgctg cgtgacggcg tggcgccggg gatgccgatc atcctctcgg
     1321 cgccggctta ttgggagccg gccaccacgc tcggcgtgaa ctgggcccac tccacgttcc
     1381 gccccaacct ctggtatgcc accgatcgct tccgggcccg gggcctggcc gacatcgtgg
     1441 acgaactggc gctcgtctcg cgcgatgcca atcccgcggc catccgcgag atcatgatcg
     1501 gcgtgaggcg ggccactcgc caccagcgac ccgtggggct catcctcctg gtggaacagt
     1561 tccagaaccg gcccgggcgc ctcctcgaag ccgtgcagac gttgaacaac ggcggctttg
     1621 gggtgacggt gagcgacgcc gggcagatcg gggcctacgg catgtgggac gtgctggcgg
     1681 aagcgatccg gccgaaccct ccgaaaccgg actcacctga cggcgggggg cggtaatgcg
     1741 gccggtcctg gtccgaacgc tcctcgcatt ccccatgctt ctcgtggcgt gctcatcggg
     1801 cgacaagacc ttgaccgaag ccgacggcgg ggttgcaccg gccgatccct cctacgagac
     1861 ggtcttcacc atcctcgacc gcaactgtgc ggcctgccat cacgattccg aggacgccgg
     1921 ggccggcccg cgattcctcg aagacgatcg cgactcctcg acgtgcgcgg gcatcgagcg
     1981 tgggctggac gacatcctcg attcggccgt cgacaacgaa cggatgccgc ccggagcctg
     2041 gccccgactg acggaggaag agaagctggt catccggctc tgggttgagg agggggcctg
     2101 ctcaccgtgt cgtccct
//
LOCUS       DASVOD010000049         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_785720, whole genome shotgun sequence.
ACCESSION   DASVOD010000049 DASVOD010000000
VERSION     DASVOD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785720"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF720_01005"
     CDS             complement(<1..399)
                     /locus_tag="VF720_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877952.1"
                     /translation="MNRIGRLLFPASSLLLGVALATPAGALPPFLVESADPSSGFVGQ
                     YSSIVLDALGEPHVSYYDESNGNLKYAWKSGATWSSETADPSADIVGLFTSIALDAQG
                     VPHVSYYDATNGDLWYANRSGGTWTQEVVDG"
     gene            680..1681
                     /locus_tag="VF720_01010"
     CDS             680..1681
                     /locus_tag="VF720_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877953.1"
                     /translation="MKIRLLAMALALCTVCHATKALALDPYDAPSISCVSATENSLTL
                     KLCAGNSGAPAGLTIHWMTLEAYEANGNTWFSSDDPRLCKISLSGQPSLQHPGASRWE
                     LGSNGCEELTIGDINFDETGVSGQDCGLDPLECGTDYVFSAFAHAGRRMGRSDWSATT
                     VCRTADCPAEDCTYTQGFWKTHGPAPCLTGNNSNVWPVGGLTLGTVNYTAVQLCAILQ
                     KPAAGNALVSMAHQLIAAKLNTLTGATCATAAAKIAGADAAIGARVIPPIGTGSLTNS
                     QTSSFTGALTTFNEGAMSTCPAHCGDEIGFHPAGDGVAPSTWGDIKSRYIGKADQAE"
     gene            2135..>3312
                     /locus_tag="VF720_01015"
     CDS             2135..>3312
                     /locus_tag="VF720_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7877954.1"
                     /translation="MFLGLAVPAGAQSINATTYPFFSSTGATLEDMSASTTQLIAANS
                     DDNASTVTDIGFDFWFGGTRYTQFSVNANGLMRLGSLVVGGNFVNDLTSSTNVPQIAP
                     YWDELMVGTNGKVHWRVAGSAPNRKLVVEWLNMQVPRVGSGNAGAATFQCWLSETTGA
                     VEIVYGEGMATNATNGGATIGVGSSASQFASVTSAGPTVSYIVSNNANVAPIASGTRY
                     ALAPPTPAAPTNLAFTGVTATTMTLNWTDNASAEVGYAVYRSLDGVTYTYLTQLPANT
                     TSYPQAGLDQSTTYWWIVRAVGEGALSATLAGSQPTTAPGMITTSGSGNWSSTVPDAP
                     WPGGVVPTASDDVTIADGTSVIVDVAASCLSLTAGQGSSGRIEFEATSARTLTVGGSL
                     S"
BASE COUNT          603 a         1096 c         1047 g          566 t
ORIGIN      
        1 cccgtccacc acctcctggg tccaggtgcc gccggaccgg ttggcgtacc agagatcgcc
       61 gttggtggcg tcgtagtagc tgacgtgcgg cacaccctgg gcgtccaggg cgatggaggt
      121 gaagagaccg acgatgtctg ccgacgggtc ggcggtctcg gaactccagg tggctcccga
      181 tttccacgcg tatttcagat tcccgttcga ctcgtcgtag tagctcacgt ggggctcccc
      241 gagcgcgtcg aggacgatcg agctgtactg gccgacgaat cccgaggaag ggtcggctga
      301 ttcgaccagg aagggcggaa gggcaccggc cggggtggcc agggccaccc cgagcagcag
      361 gctcgaggca ggaaagagaa ggcggccaat gcgattcacg taatcctccc atcgggtggt
      421 tcaaggtgtg cctggtgggg gggggaagag aatcccgacc cgttcgatcc ggaagaccgg
      481 accgacgact gactcctaac aatgcgagat gaagggcatc gggcgccaat agaattctct
      541 tacaccacgt aagaaaactc taatgatacg aagagattac taacttgctg ctgcgttcca
      601 atcacggcat aaatctctcc aggcaacaac ggcccgccgc tgggtcgtcg ggctgattca
      661 ctcaagacgg ggggggttca tgaagattcg tctactcgcg atggcgctgg cactgtgtac
      721 cgtgtgccat gcaaccaagg ccctggcact cgatccgtac gacgcgccga gcatcagctg
      781 cgtcagtgcc accgagaact ccctcaccct caagctctgc gccggtaatt ccggcgcgcc
      841 ggccggcctc acgatccact ggatgacgct cgaggcgtat gaggccaacg ggaatacctg
      901 gttctcatcg gacgacccgc gcctctgcaa gatctccctg tcgggccagc ccagcctgca
      961 gcacccgggc gcctcgcgct gggagctcgg ctccaacggc tgcgaagagc tcacgatcgg
     1021 cgacatcaac ttcgatgaaa ccggcgtgtc cggccaggac tgcggcctcg acccgctcga
     1081 gtgcggcacg gattacgtct tctcggcctt tgcccatgcc gggcgccgca tgggtcggtc
     1141 ggactggtcg gcaacgaccg tctgccggac ggccgactgc ccggccgaag actgcaccta
     1201 cacgcagggg ttctggaaga cccacgggcc ggcgccctgc ctgaccggca acaacagcaa
     1261 cgtgtggccc gtcggcggtc tcaccctggg aaccgtgaat tacaccgcgg tacagctctg
     1321 cgccatcctg cagaagcccg ccgctgggaa cgcactcgtc tcgatggctc accagctgat
     1381 cgccgccaag ctgaatacgc tcaccggcgc gacctgtgcc acggcggccg cgaagatcgc
     1441 gggcgcggat gccgccatcg gcgcccgtgt cattcccccg atcggaacgg gatcgctcac
     1501 caacagccag accagcagct tcaccggcgc tctcaccacg ttcaatgaag gcgccatgag
     1561 cacctgcccg gcgcattgcg gcgacgaaat cggattccat ccggcaggcg atggggttgc
     1621 cccgagtacg tggggcgaca tcaagtctcg ctacatcggg aaggccgacc aggccgagta
     1681 gtggggaggg gttggtccta gggccgctga ggactacgac cggcggggag gcgtcgagag
     1741 acgcctctcc gcttttcgtg tttctcgccg gcacatgaat aaacgggcaa gtcgtggatc
     1801 gatggccatt gatatcgtcg aatatcgcat ttgatcatgg acggaatcgt acttcgacca
     1861 taaactcgat gatctgccca ggccgcgcac tgcccgccgg cttgtcccga agtccagcat
     1921 tgccacaaca ccacatgagg gagagatgtg ccgccccacg tttcgtgggt ctccctcggt
     1981 gcgcgggcgg ttgtgccttc cgcattctgc accgaggcgt ccgggacctg attccaatcg
     2041 aatcctggcc ttgctgacag gcgtgtgtcg acgagccctc cagtcatgcg gggcgtcgcg
     2101 acgcccgcgc ctggccgtgt ctccgtggta tttcgtgttc ctcggccttg cggttcccgc
     2161 cggcgcccaa tcgatcaatg ccacgaccta tcccttcttc tccagcaccg gcgccaccct
     2221 cgaagacatg tcggcgagca ccacccagct gatcgccgcc aattccgacg acaacgcctc
     2281 caccgtcacc gacatcgggt tcgacttctg gttcggcgga acgcgctaca cgcagttctc
     2341 ggtcaacgcc aatggcctca tgcggcttgg gagccttgtc gtgggcggca acttcgtcaa
     2401 tgacctgacg agcagcacga acgtgccgca gatcgcccct tactgggacg aactcatggt
     2461 ggggacgaac ggcaaggttc actggcgcgt ggccggcagt gccccaaacc ggaagctggt
     2521 cgtcgagtgg ctgaacatgc aggtgccccg cgtcggctcc gggaatgccg gggctgccac
     2581 gtttcagtgt tggctctccg agactaccgg agccgttgag atcgtctacg gagagggcat
     2641 ggccaccaac gccaccaacg gtggcgccac gatcggggtc ggcagttcgg cctcgcagtt
     2701 cgcaagtgtc acgagcgcgg gccccaccgt ttcctacatc gtgtcgaaca atgccaacgt
     2761 ggccccgatc gcgtcgggaa cccggtatgc cctggcgccg ccgacgccgg ccgccccgac
     2821 caaccttgcc ttcaccggtg tcaccgcaac cacgatgacc ctgaactgga cggacaacgc
     2881 ctcggccgag gtgggctatg cggtctatcg ctcgctggac ggcgtcacct acacctacct
     2941 gacccagctt ccagccaaca ccacgtcgta tccccaggcg ggactggacc agtccacgac
     3001 ctattggtgg atcgtgcgag cggtcgggga aggagcgttg agcgccacgc tggccgggag
     3061 ccagccgacc accgcccccg ggatgatcac gacctcggga agcggtaact ggagctcaac
     3121 ggttccggat gcgccgtggc ctggcggcgt ggtcccgacg gcctcggacg acgtgaccat
     3181 cgcggacggc acctccgtga ttgtcgacgt ggcggcttcc tgcctctcgc tgacagccgg
     3241 ccagggcagt tcagggagga tcgagttcga ggcgacgtcg gcccggacgt tgacggtcgg
     3301 gggaagcctg tc
//
LOCUS       DASVOD010000050         3726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_159846, whole genome shotgun sequence.
ACCESSION   DASVOD010000050 DASVOD010000000
VERSION     DASVOD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159846"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VF720_01020"
     CDS             <1..480
                     /locus_tag="VF720_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877955.1"
                     /translation="AEKILVAHLPLANATFLFDRHALPAEELRCDEGFRRCCQDADLL
                     MRLLRRHPSVLLPEVHVHQRRYPGQESATGNPAAMVPDMRRLLRQHRSLLFREAPFLA
                     ALGTWVRAWSTQYHFEALVGRGTWRGYGFMALSAILWPGNPRLRGTLGRRLAGRPTG"
     gene            566..2791
                     /locus_tag="VF720_01025"
     CDS             566..2791
                     /locus_tag="VF720_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX7877956.1"
                     /translation="MRYQQFRRSSSFRFGWPTNPVGRDFPLRILLVLIVLGLPGLVAP
                     LANGQTASPNAYPVPPGVYPVPTSTPIADKTRLQSTTEMSYADVYRLLLSRGLTEEAA
                     NELMRDYDPDRKFTPGEVSDILRRVRPEDLSTRRKKEETAAEVEASAVVPQQLGVSPE
                     PVPFGYEVFVGAPESYEPEQDLAVGNDYRLGPGDELKISVWGSVTQDFFAVVDREGNV
                     TIPEVGVLPAAGKTMTEFEAELDRELRKVFSGFKLGVTLRRLRRIQVVVAGDVARPGA
                     YFLSPVSTAFNALYFAGGPTPEGSLRRVRVVRDGVIVAEVDLYPYLMFGDTSNQVRLL
                     SGDTVFIMHKGPVATLRGEVNRPAIFELLGRETVADLVRMGAGFTPTVHPERATLDRV
                     SPATGPMSVELNLTGLLRPDSTLAADDPALLPLQAGDNLTLYSIYHVEPRRFVEIQGM
                     VQYPGIYPLFPDSRVSDLVYRAGGLLDSAYPIRAELSRLALAGGDTLADDGDEAQVSR
                     LIYVELSEALAHPRSESDLTLQKGDKLYIRKIPGWKFQETVKIAGEVKYPGIYPILLR
                     EERLSDFIQRAGGVTDESFLKGASLFRKGQGRVIIDFEKALKKTGDRENIVLADEDSI
                     FVPPYPPTILVEGEVSQPGALIYKPGQTADYYVDRTGGVTDDADKGETRIIRVDGIVQ
                     KAFRTLARDPKVEPGSRIVVAAKPEGEGVNWGNVFKDTVTILASLATTIFVITEINNN
                     N"
     gene            2795..>3726
                     /locus_tag="VF720_01030"
     CDS             2795..>3726
                     /locus_tag="VF720_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877957.1"
                     /translation="METPPTSGPIAAPGARTWPLLETLAIILAWRRVVIAFAAGVLLV
                     TLVYSLVAKPVFLVRSSLLPKQEETGMMGLSSLVANQFGALAGGISGITSDTDVLMSI
                     LESDEIRDRVINRLDLVRVLKIKAKTPARAREMAQERLQRLVRVDLTRRSSIFLEARA
                     PTAQLAADIAAAYFEELDNLNQEFAFTSARQTRMFIEKRLAETRDTLSVAQTRLEDFQ
                     REHGMVSLDEQAKAEVEVVSKLEAELITMEAQLEVQRQYSTGSFSGTRELQFKIAALK
                     NRLAQILGGDAPSASEPRSSLMISFRRMPGLGRQL"
BASE COUNT          628 a         1306 c         1180 g          612 t
ORIGIN      
        1 gccgaaaaaa tcctggttgc gcacctgccc ctcgccaacg ccacgttcct cttcgaccgc
       61 cacgcgctcc cggcggagga gctccgatgc gacgagggat tccgccgctg ctgccaggat
      121 gccgacctcc tgatgcgact cctgcgccgc cacccctcgg tgctcctccc cgaggtgcac
      181 gtgcaccagc ggcggtatcc cggccaggag tcggccaccg gcaacccggc cgccatggtc
      241 ccggacatga ggcggctcct tcggcagcac cggtcgctcc tcttccggga ggcgccgttc
      301 ctggccgccc tcggcacctg ggtgagggcc tggtccaccc agtaccactt cgaggccctc
      361 gtcggccggg gcacctggcg gggctacggg ttcatggccc tcagcgccat cctctggccg
      421 ggaaatccgc ggctccgcgg cacgctcggg cgtcggctgg ccggccgacc gacggggtga
      481 cgcccggaaa cttgactctc cccccgggcc cccgtaatct cattgggttc ctgaatgcat
      541 tatctcaagc aacggggctt tctgattgag ataccagcag ttccggcgtt cgagttcctt
      601 ccgcttcggg tggcccacga accctgtcgg ccgcgacttt ccgctgcgaa tcctcctcgt
      661 cctgatcgtt cttggcctgc cgggactcgt cgcgccgttg gcgaacggac agaccgcgtc
      721 acccaacgcg tacccggtgc caccgggcgt ctacccggtg ccgaccagca cacccatcgc
      781 ggacaagacc cggcttcaat ccaccaccga aatgtcctac gccgacgtct atcggctgct
      841 cctgtcgcgc ggcctcaccg aagaggcggc caatgaactg atgcgcgact acgatcccga
      901 ccggaagttc acccccggcg aggtgagcga catcctccgc cgcgtccggc cggaggatct
      961 ctccacccgc cgcaagaagg aagagacggc cgccgaggtc gaggcctccg cggtggtgcc
     1021 ccagcaactc ggtgtctcgc cggagccggt gcccttcggt tacgaggtgt tcgtcggggc
     1081 gcccgagtcg tacgaacccg agcaggacct ggcggtaggc aacgactacc gtctcggccc
     1141 gggggatgaa ctcaagattt cggtctgggg atcggtcacg caggacttct tcgcggtcgt
     1201 cgatcgcgag ggaaacgtca ccatcccgga agtgggggtg ctccccgcgg cggggaagac
     1261 gatgaccgag ttcgaggccg aactcgatcg tgaacttcga aaggtcttct cgggattcaa
     1321 gctcggcgtg acgctgaggc gcctgcgccg catccaggtg gtcgtggccg gcgatgtcgc
     1381 tcggcccggt gcgtacttcc tgtcgccggt ctccaccgcg ttcaacgccc tctacttcgc
     1441 cggcggcccc acgcccgagg ggagcctgcg tcgtgtccgc gtggtgcgcg acggggtcat
     1501 cgtcgccgag gtcgacctgt atccgtacct gatgttcggg gacacgtcga accaggttcg
     1561 ccttctctcg ggtgacacgg tgttcatcat gcacaagggt cccgtggcca cgctgcgggg
     1621 cgaggtgaat cgcccggcaa tcttcgaact cctgggacgg gaaaccgtgg ccgacctcgt
     1681 gaggatgggc gccggcttca cgcccactgt ccatcccgag cgggccacgc tcgaccgggt
     1741 ttcgcccgcc accggcccga tgagcgtcga actcaacctc acgggactcc tgcgcccgga
     1801 ctccacgctg gcggcggacg atcccgccct cctgccgctc caggccggcg acaacctgac
     1861 gctctactcc atctatcacg tcgagccgcg gcgcttcgtg gaaatccagg ggatggtgca
     1921 gtacccgggc atctacccgc tctttcccga ctcccgcgtc tccgacctgg tgtaccgggc
     1981 cggcggcctt ctcgacagcg cctatccgat ccgggccgag ctctcgcgcc tcgcgctggc
     2041 cgggggggac acgctggccg acgacgggga cgaggcgcag gtgagccggc tcatctacgt
     2101 cgagttgtcc gaggcgctgg cgcatccccg ctcggaaagc gacctcaccc tccagaaagg
     2161 ggacaagctc tacatccgca agatccccgg ctggaaattc caggagacgg tcaagatcgc
     2221 gggcgaggtc aagtatccgg gcatctaccc gatcctgctg cgcgaggagc gcctctccga
     2281 cttcatccag cgggccggcg gcgtcaccga cgaatcgttc ctgaagggcg cttcactgtt
     2341 ccggaagggg caggggcgcg tgatcatcga cttcgagaag gccctcaaga agaccggcga
     2401 ccgggaaaac atcgtcctgg ccgacgagga ttcgatcttc gtgcccccct atccacccac
     2461 catcctggtg gaaggggagg tcagccagcc gggcgccctc atctacaagc ccggccagac
     2521 ggccgactac tacgtggacc gcacgggcgg cgtgacggat gacgccgaca aaggggagac
     2581 gcgcatcatc cgggtggatg gcatcgtcca gaaggccttc cgcacgctgg ccagggaccc
     2641 caaggtcgag cccggatccc gcatcgtggt ggccgccaag cccgagggcg aaggggtgaa
     2701 ctggggaaat gtcttcaagg acaccgtcac catcctcgcg agcctggcca cgaccatctt
     2761 cgtcatcacc gaaatcaaca acaacaacta ggacatggaa accccaccca cctcgggacc
     2821 gatcgccgct cccggggcgc gcacctggcc gctgctcgag acactggcca tcatcctggc
     2881 ctggcgtcgg gtcgtcatcg cgtttgccgc gggcgtgctg ctggtcacgc tggtctactc
     2941 gctcgtggcc aagccggtct tcctggtgcg ttccagcctc cttccgaagc aggaggaaac
     3001 ggggatgatg gggctctcca gcctcgtggc caaccagttc ggcgcgctgg caggcgggat
     3061 ctcgggcatc acctccgata ccgacgtact gatgagcatc ctcgagagcg acgagatccg
     3121 cgatcgcgtc atcaaccggc tcgacctggt gcgggtgctc aagatcaagg caaagacacc
     3181 ggcgcgcgcc cgggaaatgg cacaggagcg gctccagaga ctggtgcgtg tcgacctcac
     3241 ccgtcgctcc tcgatcttcc tcgaagcgcg ggcgcccacc gcccagctgg ccgcggacat
     3301 cgcggccgct tatttcgagg agctcgacaa cctgaaccag gagtttgcct tcacctcggc
     3361 ccgccagacg aggatgttca tcgagaaacg tctcgccgaa acgcgggaca cgctcagcgt
     3421 ggcgcagacc cgcctcgagg atttccagcg cgagcacggc atggtgtcgc tcgacgagca
     3481 ggccaaggcc gaggtcgagg tcgtttcgaa actggaagcc gagttgatca ccatggaagc
     3541 ccagctcgag gtgcagcgtc agtattccac cggttcgttt tcgggcaccc gggaactcca
     3601 gttcaagatc gcggcgctca agaaccgcct ggcacagatc ctgggcggcg acgcaccgag
     3661 tgcgtctgag ccacgcagca gcctcatgat ctcgttccgg aggatgccgg ggctcggtcg
     3721 ccagct
//
LOCUS       DASVOD010000051         6234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_316093, whole genome shotgun sequence.
ACCESSION   DASVOD010000051 DASVOD010000000
VERSION     DASVOD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316093"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VF720_01035"
     CDS             <1..1228
                     /locus_tag="VF720_01035"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7877958.1"
                     /translation="FEACNGGLATGQFYPGFAVANGDTIPAIGGLQDNFSAIYEGSPA
                     WTRVIGGDGCWAAIDPANPFNMWASYQLLSILRSFDRGANWIEAIPPGGGSTTAFVAP
                     YVLSPFSNTTLYAGRAIVYRSTNGGTSWTAGGAIAGRATISMFASPTQAGRIVVGCEP
                     DASGNSVWLSDNDGATWTNISAGLPNRYPLDVVIAPDNSATIYVTFGGYDIPHVWRST
                     NSGGTWQNIGNALPDLPTWALAVDPLHPSHLYVGNDLGVFVSPDGGLTWGSFREGLPE
                     AVMVSDIVVTPGVRRLRIATHGNGVFDRPIPDLITGVPERPAKPVAHLAAWPNPFSGA
                     VRLAVRGADDLAEAEIVIVSVDGRVVRRLPPGETGSDVRWDGNDDSGRPVSEGVYWAR
                     VTGRPGIAASRLVRLR"
     gene            complement(1236..1865)
                     /locus_tag="VF720_01040"
     CDS             complement(1236..1865)
                     /locus_tag="VF720_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877959.1"
                     /translation="MRASILVTVIAGSLLAAAMSVLVNPRSASLVSWLTHRPATTTEM
                     LALPDLAMEGSDAAVSPAENETTNGIEPPNVLTLPAGTVIRALLQYPIGSVVSRAGDA
                     VTLSVIEPVLVGGAVAIPRGASVQGVVNEARGAEGGGGVGLLSLTYKSVTDVGGKMHG
                     IKARHLRTTDLLPAGAARHGDVELQRGDILEVRLEAPLVVAVGAGRTSS"
     gene            2386..3960
                     /locus_tag="VF720_01045"
     CDS             2386..3960
                     /locus_tag="VF720_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7877960.1"
                     /translation="MTTLPVHDERPTTGAHRAGEPKMTASRILIVDDEAPIREGLKEL
                     LCGWGYDTRSASDSKAAMEIAETFSPQLILSDLMMPGLSGLELMAQVREQSPNVAFIL
                     LTGHGTIENAMQAMREGAHDYLTKPVDLDRLRVVIERALEIVRDRDELVRLTNDLADR
                     GQFGRLVGSSPAMRKVYRVVQQAAPSRASVLITGESGTGKEVVARTIHEMSARRNKPF
                     VAVNCAAIPETLLESEILGHEKGSFTGATSARAGCFEQANGGTLLLDEIGEMPPELQA
                     KLLRVLEERTLRRVGGRQEFPVDVRVLSSTNVNIETALREGKFREDLFYRLNVFTIAL
                     PSLRERPEDIGLLMQHFVEEFAESYDKTIRGITPAALARMRQHDWPGNVRELRNVVER
                     AVVVAHRDIIDVDDLPAFGPSSSRLPSPADESGRAEIISGIDWGSAQGRGYEVRDSGG
                     EAVPMAATGTHGGATSGNGTVTLPVGTTVEEAERVLIFETLKMTNQNKTRAAEVLGIS
                     TKTLHNKLKLYRRSDP"
     gene            3957..5834
                     /locus_tag="VF720_01050"
     CDS             3957..5834
                     /locus_tag="VF720_01050"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7877961.1"
                     /translation="MTTEIWPRFAEIIRRIVFLRPGLRLALILAVALFALVVGIGSAL
                     VEISTITRLEVRAAISRGELANSLIISQLGQAVINSPGRSLHDAIAYDERVGTTLEDA
                     RGFIPGLVEVTITDTANVVMRSTQPELAGRLALPREELEPADGLGLLRQGFSLLRVTE
                     PFEVATGFEVDGRQVGTVRALISPGLMREELKQVFDTHLVKLGLQVLLATIFAFAVSW
                     LVVLKPLGRIGDALGRLQRGEYSGLLEEDDPDDIGNISRQINELAARMAQQRQEFMHT
                     RDEGDALRRLVNAVDDGLIMIDADRKILMANEQAIAHLGGDAEELVGRDLRQILPPES
                     PLLSAVNAAFAGVPLESSPMEFMTPTGPRMLLATCQMADDATGRSVAMISLRDYGRIM
                     RIREMIDHARVLSRIGQMAAGVAHEVRNPLNAMNIHLQLLKEKMPAGNGDGGRPHLVV
                     VQDEIARLERVVSGFLKIARNQELSYKRIKVEPFLDKLVELVQPAARMAGIRVDLKVF
                     PGVPDLYGDQEVLHQAFLNLVLNAIQASTAGSGPVTIAASPDANQVCITVSDHGRGMT
                     EEVLEQAFKLYFSTRKEGSGVGLALVQQSVEMHGGRVEVESEPGKGTRFTICIPAVDA
                     G"
     gene            5824..>6234
                     /locus_tag="VF720_01055"
     CDS             5824..>6234
                     /locus_tag="VF720_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877962.1"
                     /translation="MRADDASRSAVGSAAHAARGLAGLALIACLPLALTGCSLFHRGD
                     PPAVEVVAPEPTPDTTAVTVTPPAPAPTRSKSGSSKKNGSTKSTSKPTPAPPPPDTLA
                     AVHPPPAPELADAAHRPVFSAVLTEEQKNDKAARF"
BASE COUNT         1079 a         2035 c         2091 g         1029 t
ORIGIN      
        1 gttcgaggcc tgcaacgggg ggctggccac cgggcagttc taccccggct tcgccgtggc
       61 caacggggac acgatcccgg cgatcggcgg gctgcaggac aacttttcgg ccatctacga
      121 ggggagcccg gcctggacgc gcgtgatcgg cggtgacggc tgctgggcgg cgatcgatcc
      181 ggccaacccg ttcaacatgt gggcctcgta ccagctgctc agcatcctcc gctcgttcga
      241 tcgcggggcc aactggatcg aggcgatccc tcccggggga ggttccacga cggcgttcgt
      301 ggccccctat gtcctctcgc ccttttccaa taccacgctc tacgccggcc gggcgatcgt
      361 ctaccgcagc acgaacgggg gcacgagctg gaccgccggc ggcgcgatcg cgggacgtgc
      421 caccatctcg atgttcgcct caccgacgca ggcgggacgc atcgtggtgg ggtgcgagcc
      481 cgatgcctcc ggcaattcgg tctggctctc ggacaatgac ggagccacct ggaccaacat
      541 ttccgcgggg cttcccaacc gatacccgct cgacgtggtg atcgcgcccg acaattccgc
      601 cacgatctat gtcacgttcg gcggttacga catcccgcat gtctggcgat cgacgaacag
      661 cgggggcacc tggcagaaca tcggcaacgc gctccccgac ctgccgacct gggcgctggc
      721 ggtcgatccg ctccacccct cgcacctcta cgtcgggaat gatctcggcg tgttcgtgtc
      781 gccggatggc gggctcacct ggggatcgtt ccgcgaagga ttgccggagg ccgtgatggt
      841 gagcgacatc gtggtgaccc ccggcgtgcg gcgactccgg atagccacgc acggcaacgg
      901 ggtcttcgat cggccgattc cggacctcat caccggcgtg cccgagcggc cggcgaaacc
      961 ggttgcccac ctcgccgcat ggcccaatcc cttctccggt gcggtgcgac tggctgtccg
     1021 cggcgccgac gaccttgccg aggccgagat cgtgatcgtg agcgtggacg ggcgggtggt
     1081 gcggcggctc ccgcccggcg agacggggag cgacgttcgc tgggatggaa acgacgattc
     1141 cggccgaccc gtgtcggagg gtgtttactg ggcgcgggtc acgggccggc cgggaatcgc
     1201 cgcaagtcgg ctggttcggt tgcgctgagc cggactcaac tcgaggttcg gccggcgccc
     1261 accgcaacga ccaggggcgc ctcgaggcgc acttccagga tgtccccgcg ctggagttcg
     1321 acgtcgccgt ggcgcgccgc gccggccggc aacaggtcgg tggttcgcag gtggcgggcc
     1381 ttgatgccat gcatttttcc gcccacgtcg gtgacggact tgtaggtcag cgacaggagc
     1441 cccacaccgc cacctccctc cgcgccccgg gcttcgttga ccacaccctg caccgacgcc
     1501 ccgcgcggga tggccacggc ccccccgacg agaaccggct cgatgaccga gagcgtgacg
     1561 gcatcacccg cgcgactgac gacggagccg atgggatact ggagcagggc gcggatgacg
     1621 gtgccggcgg gaagcgtgag gacgtttggg ggctcgatcc cgttcgtcgt ttcattttcc
     1681 gccggtgaca cggcggcgtc cgacccttcc atcgcgagat ccggcagggc cagcatctca
     1741 gtcgtcgtgg cgggtcgatg ggtgagccat gagacaaggg aggcggagcg gggattgacg
     1801 agaacggaca tcgcggcagc gaggagcgat ccggcgatga cggtgacgag gatgctggcg
     1861 cgcatcgatc ctcggcgggg gggagactag gggaggtcgg gcgccgcaaa ggggctatct
     1921 ccgttgcggt gggcgcccga ccaccctgag tcccgggtat ggggatgggg ttggtgaatt
     1981 ccggaccggg tcgactcttt ggatctggcc gcgtgaccgc cgagtcgacc cagtccagtc
     2041 ttcgtgggga gcagtttgca agaggggtgc caatcaccag tcgacgaaga atcaacgact
     2101 tacgaggttc gtggggtcac aaagtgtgta gagataacgc gatggggaaa tttcgtggac
     2161 ggcgaagagg gtaattacgt catagtggga aaaacagggg gtaatttttt ccgacccttc
     2221 ttgcgccgac gagcgggaat tttttacccg gcgccaatgg gatcggggtg tgcctgatcg
     2281 ccgaagttgt ttgccggcaa aaacttcgaa gtttctccca agttcggcac acccctcgca
     2341 atgggtgaga aaagcgacca ccctgtccga agggagatac ctatcgtgac caccctgcct
     2401 gtgcacgacg agaggccgac gaccggggcc catcgtgcgg gagagccgaa gatgacggcc
     2461 agtcggattc ttatcgtcga tgacgaagca ccgatccgcg aagggctgaa agaactcctt
     2521 tgcgggtggg gatatgacac ccggagtgcc tccgactcca aggccgccat ggagatcgcc
     2581 gagacttttt cgccacagct gatcctgtcc gacctgatga tgccgggact ctccggtctc
     2641 gaactgatgg cccaggtcag ggaacagtcg cccaacgtgg cgttcatcct tcttaccggc
     2701 catggaacga tcgaaaacgc catgcaggcc atgcgcgaag gcgcacatga ttacctcacc
     2761 aagccggtcg acctggaccg tcttcgtgtc gtcatcgagc gtgcgctgga aatcgtccgc
     2821 gaccgtgacg agctcgtacg cctcaccaac gacctggccg accggggcca gttcgggcgc
     2881 ctggtgggaa gttcaccggc catgcgcaag gtgtatcgcg tcgtccagca ggcggcgccc
     2941 agccgggcca gcgtactcat caccggcgag agcggcacgg gcaaggaagt cgtggcccgc
     3001 acgatccacg agatgagcgc acgccgcaac aagccgttcg tggccgtgaa ttgcgcggcc
     3061 atcccggaga cgctcctcga aagcgagatc ctcggccacg agaagggatc attcaccggc
     3121 gccaccagcg cgcgggccgg ttgcttcgaa caggccaatg gtggaacgct gctcctggac
     3181 gagatcggcg agatgccgcc cgagctccag gccaagctcc tgcgcgtcct ggaggagagg
     3241 acgctccggc gggtcggcgg gcgccaggag ttcccggtgg atgtccgcgt cctctcctcg
     3301 accaacgtca acatcgagac ggcgctgcgc gaggggaagt tccgcgagga cctcttctac
     3361 cggctgaacg tcttcaccat tgcgctgccg tcgctgcgtg aacgtccgga ggacatcggc
     3421 ctgttgatgc agcacttcgt ggaagagttc gccgagtcgt acgacaagac catccgcgga
     3481 atcacgccgg cggcactggc ccgcatgcgg cagcatgact ggccggggaa cgtccgcgag
     3541 ctgcgcaacg tggtggagcg cgccgtggtg gtggcccatc gggacatcat cgacgtcgac
     3601 gacctgccgg ccttcggtcc atcgtcttcg cgtctaccct ccccggccga tgagagcggc
     3661 cgggccgaga tcatttccgg aatcgactgg ggctcggcgc agggtcgcgg ctacgaggtg
     3721 cgcgacagcg gcggggaagc ggtgcccatg gcggccacgg gcacgcacgg tggcgccacg
     3781 tccggcaacg gcacggtgac cctgccggtc ggcacgaccg tcgaggaagc cgagcgggtg
     3841 ctgatcttcg agacgctcaa gatgaccaac cagaacaaga cacgcgccgc ggaggttctc
     3901 ggcatcagta ccaagacgct ccataacaag ctgaagttgt accgtcggtc ggatccgtga
     3961 ccacggagat ctggccgcga ttcgcggaga taatccgccg aatcgttttt ctgcgacccg
     4021 ggctgcggct ggcattgatc ctggccgtgg cccttttcgc gctggtggta ggaatcggct
     4081 ccgcgctggt cgagatcagc accatcaccc gcctcgaagt aagggcggcg atcagccggg
     4141 gcgagctcgc caactccctc atcatttccc agctcggcca ggcggtcatc aactcgcccg
     4201 gtcgatcgct ccacgacgcc atcgcctacg atgagcgtgt gggcaccacg ctggaggatg
     4261 cccgcggttt catcccggga ctggtggagg tcaccatcac cgacacggcg aatgtcgtga
     4321 tgcgatccac ccagccggag ctggccgggc gcctggccct tccacgggag gaactcgaac
     4381 cggccgacgg gctcggcctc cttcgccagg gattctccct gctccgggta acggagccgt
     4441 tcgaagtggc cacgggtttc gaagtggacg ggcgccaggt gggaacggtg cgggccctga
     4501 tctcccccgg cctcatgcgc gaggagctca agcaggtctt cgacacgcac ctggtcaagc
     4561 tcgggctcca ggtgctcctg gccaccatct tcgcttttgc cgtgtcgtgg ctggtggtgc
     4621 tcaagccgct ggggcggatc ggtgatgccc tcggacggct tcagcgcggg gagtacagcg
     4681 ggctgctgga agaggacgac cccgacgaca tcggcaacat ctcccgccag atcaacgagc
     4741 tggccgcgcg aatggcccag cagcgacagg aattcatgca cacgcgcgat gagggtgatg
     4801 cgcttcgccg gctcgtcaac gccgtcgacg acggcctcat catgatcgat gccgaccgga
     4861 agatcctgat ggcgaacgag caggccatcg cccatcttgg aggcgacgcc gaggaactgg
     4921 tgggacgcga tctccgccag atcctgccgc cggagagccc gctcctctcg gccgtgaacg
     4981 cggcattcgc cggtgtcccg ctcgagtcgt caccgatgga gttcatgacg ccgaccggcc
     5041 cccggatgct cctggccacc tgccagatgg ccgacgacgc cacgggacgc tcggtggcca
     5101 tgatctcgtt gcgcgattac gggcgcatca tgaggatccg cgagatgatc gaccacgccc
     5161 gggtgctctc ccgcatcggg cagatggctg ccggggtggc ccacgaggta agaaatccac
     5221 tcaacgccat gaacatccac ctgcagctcc tcaaggagaa aatgcccgcc ggcaacgggg
     5281 atgggggaag gccccacctc gtcgtggtgc aggacgaaat tgcccggctc gagcgggtcg
     5341 tttcgggctt cctcaagatc gcgcgcaacc aggagctcag ttacaagcgg atcaaggtgg
     5401 agcctttcct cgacaagctg gtggaactgg tgcagccggc ggcgcgaatg gccggaatcc
     5461 gggtggacct caaggtgttc ccgggtgtgc cggacctgta tggcgatcag gaggtactcc
     5521 accaggcctt tctcaatctg gtgctgaatg cgattcaggc ttccacggcg ggttccggac
     5581 ccgttacgat tgccgcctcc cccgacgcca accaggtttg catcaccgtc tccgaccatg
     5641 gtcggggaat gacggaggag gtcctcgagc aggccttcaa actctacttt tcgacgcgta
     5701 aggaggggag tggtgtcggt ttggcgctgg tgcagcaatc ggtggaaatg catggggggc
     5761 gtgtcgaggt cgagtccgaa cccggcaagg gaacccgctt caccatctgc atcccggcgg
     5821 tcgatgcggg ctgacgacgc gtcgcgttcc gccgtcggct ccgcggctca tgccgcgcgc
     5881 gggcttgcgg gcctcgcctt gatcgcgtgc ctacccctcg cgctgaccgg ctgctctctc
     5941 ttccatcggg gcgatcctcc cgccgtggag gtcgtggctc ccgaaccgac cccggacacg
     6001 accgcggtaa ccgtgacgcc tcccgcgcct gcgccgaccc ggtcgaagag cggctcgtcg
     6061 aagaagaacg gatcgaccaa atccacctcg aagcccacgc cggccccgcc acctcccgac
     6121 acgctggcgg ccgtccatcc cccacccgct cccgaactgg ccgacgccgc gcaccgcccc
     6181 gtcttcagtg ccgttctcac cgaggaacag aagaacgaca aggcggcccg attc
//
LOCUS       DASVOD010000052         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_473168, whole genome shotgun sequence.
ACCESSION   DASVOD010000052 DASVOD010000000
VERSION     DASVOD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473168"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VF720_01060"
     CDS             complement(<1..1347)
                     /locus_tag="VF720_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7877963.1"
                     /translation="MTEPQLPLRQATTRDFLAVVFRQLWVIVAVFLIASLSVVVVSLR
                     SPTNYESDALVRVARGQKQSVLQSNLQVFPWEEEISTELETVKSLPVVQRAQKILDQW
                     HTEGRFNNKFRVSPSGVGAGLVGESNVIIISYNSRDAESCQPVTNALTQAYMDYRRDS
                     ATVPFVNEFFQREISSVDSAMAAVMQRRQSFVSETGAVDPEEEQHALFNLVQNAEAEQ
                     AQVREDVRLAQSNLAQGRKYLDGAELPDPGFFFQLNLGNQQALSKLKSDLSELEQRRE
                     SLASTQTPSHPELRAVLTSIEEVRSQIRAEARASLALLESRIATLTERERSLSSQIGS
                     YRGQLEQLPYQQNALNQLDHEMQTLRDRYKELVGKEITARIQQATSPDWTVTLYSPAS
                     RPKALRTTDYVRLALAPLLSLVVGLMLAFFLDSLDHSLKTAGDVEEYLGIPVLASLP"
     gene            complement(1349..2212)
                     /locus_tag="VF720_01065"
     CDS             complement(1349..2212)
                     /locus_tag="VF720_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX7877964.1"
                     /translation="MMIRSANRAAPGAGWWRAALVLAALAFVLSGCAGGSGVNGDVTM
                     IEEEVGKPEDTITFGPPESTYVDPSYRLGPGDTIRMVFLFDHDLDDEIIIRPDGGINL
                     PILGDLPVAGLTPGQLADTVMVAYSQYYTNPQLSINLKEFAPPKVYVLGDVKYPKAVD
                     IRPGMTVAGALAEAGGPNEFADLRNTVLVRRLSRNQAMAKRFDILRFAEGRPMSSDLY
                     LQDYDIVFIPRSFLGKLVTVVDQIFGKLVDIPIFYLRGWEAFNTDLVYNREIRPSEIP
                     NSSSPGSREAQ"
     gene            complement(2537..>3016)
                     /locus_tag="VF720_01070"
     CDS             complement(2537..>3016)
                     /locus_tag="VF720_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877965.1"
                     /translation="GRHLVESGAPHRDRSLLAGAYRKNVPLTVHVAIGTDILHQHPSA
                     DGAAIGATTLRDFRILAEAIRPLAGGVVLNLGSAVILPEVFLKVFSIHLNLGETVTEL
                     TTVDFDFLRHYRPTQNVVRRPTAGGRGEGYSLTGHHEILVPLYVAGVLRTLERAPGS"
BASE COUNT          532 a          937 c         1005 g          542 t
ORIGIN      
        1 cggcaaagaa gccaggacgg ggatgccgag gtattcctcg acgtcgccgg cggtcttcag
       61 tgagtgatcg aggctgtcca ggaagaaggc cagcatgagc ccgacgacca gggagaggag
      121 cggcgcgagc gcgaggcgca cgtagtcggt ggtgcgaagt gccttcggcc gactggcggg
      181 agagtaaagc gtcaccgtcc agtcggggct ggtggcctgc tggatccggg cggtgatctc
      241 cttcccgacg agttccttgt aacggtctcg cagcgtctgc atctcgtgat cgagctggtt
      301 cagcgcattc tgctggtagg ggagctgctc gagctgccct cggtagctcc cgatctggct
      361 ggagagggag cgctcacgtt cggtcagcgt cgcgatcctc gactcgagca gggccagtga
      421 cgcccgtgct tcggcgcgga tctgcgaacg aacctcttca atcgacgtca ggacggcccg
      481 gagctcggga tgactggggg tctgggtcga tgcgagcgac tcccgccgct gctcgagctc
      541 ggaaaggtcg ctcttcagct tgctcagcgc ctgctggtta ccgaggttca gctggaagaa
      601 gaagccagga tcgggaagtt cggctccatc caggtatttc cgaccctggg ccaggttcga
      661 ctgggccagc cggacatctt cgcgcacctg ggcctgctcg gcctcggcgt tctgcaccag
      721 gttgaagagc gcatgctgct cttcctctgg atcgacggcg ccggtctcgc tcacgaagct
      781 ctggcggcgc tgcatgacag ccgccatggc cgagtccacc gagctgatct cccgctggaa
      841 gaactcgttg acgaaaggca ccgtggccga gtcccgccgg taatccatgt aggcctgggt
      901 cagggcgttg gtgaccggct ggcagctttc cgcatcgcgg ctgttgtagg aaatgatgat
      961 gacgttcgac tcaccgacca ggccggcgcc gacgcccgac ggggacaccc ggaacttgtt
     1021 gttgaaccgt ccctccgtgt gccactggtc gaggattttc tgggcgcgct ggacgacggg
     1081 aaggctcttc accgtttcca gctcggtgga gatttcctcc tcccacggga agacctggag
     1141 gttggattgc agcacgctct gtttttggcc gcgggccaca cgcaccaggg catccgactc
     1201 gtagttcgtc gggctccgga ggctcaccac caccaccgac aggctggcga tgaggaacac
     1261 ggccacgatg acccaaagct ggcggaaaac caccgccagg aagtcgcggg tcgtggcctg
     1321 acgcaggggc agttgcggtt cggtcatgtc attgcgcctc gcgtgaaccc gggctggaac
     1381 tgttgggaat ttcgctcggc cggatctcgc ggttgtacac gaggtcggtg ttgaaggctt
     1441 cccagccgcg gagatagaaa atcgggatgt cgaccagctt gccgaatatc tgatcgacca
     1501 ccgtcacaag tttcccgagg aagctcctcg gtatgaacac gatgtcgtag tcctggaggt
     1561 aaaggtcgct cgacatcgga cgtccctcgg cgaaccggag gatgtcgaat cgtttcgcca
     1621 tcgcctgatt cctcgagagg cgacggacca gcaccgtgtt gcgcaggtcg gcgaattcgt
     1681 tcgggccgcc cgcctcggcc agcgcgcccg ccacggtcat gcccgggcgg atgtcgacgg
     1741 ccttcggata cttgacgtcg cccagcacgt acaccttggg cggcgcgaat tccttgagat
     1801 tgatcgagag ctgcgggttc gtgtagtact ggctgtaggc caccatgaca gtgtccgcca
     1861 gctgcccggg cgtgagcccc gccaccggca ggtcgccgag gatcggcagg ttgatgccgc
     1921 catccggacg gatgatgatc tcgtcgtcca ggtcgtggtc gaacaggaag accatccgga
     1981 tcgtgtcgcc gggaccgagc cggtagctcg gatcgacata ggtcgactcc gggggaccga
     2041 acgtgatggt gtcctccggc ttcccgacct cttcctcgat catcgtcacg tcgccgttca
     2101 cgccgctgcc gccggcgcat cccgacagca cgaaagccag agccgccagc acaagcgcag
     2161 cccgccacca tccagcgccc ggcgccgccc tgttcgcgga tcgaatcatc atcctcccgt
     2221 tatcggacga tgggctcccc ggttgagcgg gataccccaa acaaaaaagc cgaccccgca
     2281 cgaggtcggc cagtcgacgt ttggtccacc ggagacggtt gccgcgtgcg gcaggacgac
     2341 aggattgccc gaaagtggca agatactgtc gccaaatgag ttgaacgatc agaacgcccg
     2401 ttcagacccg ttaaccagtc tcgatccgtt gctcctgagg gtgccgctga cgacgggaaa
     2461 tggacgggaa aaagtataac aatgccctcg acggggcgtc aaatgttgga gttcaccacc
     2521 gttccgctgg gccggttcag ctccccggag ccctctcgag cgtccgcagg actccggcca
     2581 cgtagagcgg cacgaggatc tcgtggtgtc cggtaagcga gtacccctcc cccctccccc
     2641 ccgccgtcgg ccggcgcacc acgttctgcg tgggacggta gtgccggagg aagtcgaagt
     2701 cgacggtagt gagttcagtc accgtttccc cgaggttcag gtggatggag aagaccttga
     2761 ggaagacctc ggggaggatg accgcggatc cgaggttcag taccacgccg ccggccaggg
     2821 gacggatggc ctcggccagg atgcggaagt cgcggagggt ggtggctccg atcgccgcac
     2881 cgtcggcgga ggggtgctga tggaggatgt ccgtcccgat ggccacatgc accgtgagcg
     2941 gtacgttctt gcgatacgcg ccggccagga ggcttcggtc gcggtgcggc gcaccgctct
     3001 ccaccaggtg ccgacc
//
LOCUS       DASVOD010000053         1725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_212367, whole genome shotgun sequence.
ACCESSION   DASVOD010000053 DASVOD010000000
VERSION     DASVOD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212367"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VF720_01075"
     CDS             <1..905
                     /locus_tag="VF720_01075"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7877966.1"
                     /translation="VESGGRLLMRRRSEAESLMPGMWELPAWRTPDSGSIRGHVERDL
                     GGGSVAIVPAGDARHAIMNRRLRLNVHHVEMARRQAPPGWHWIEPGRLGELPVSSMVF
                     KALEAARGRLKLAFALLLAASVAMASGVPYAHGEDPAVTPAASAAVPADPDTTTIPLP
                     IVPELVVRDIAGKEVSLHALLAKGPVLLNFWALWCKPCLKEIPELVKLQENYAARGFS
                     LMLVNGDSPSDLARVAPFARARKMAGPVVTDVDGALRRRFQANAFPTSILLDRSGRTA
                     WSAQGYRPGDEVKLAAEIEALLAK"
     gene            902..1393
                     /locus_tag="VF720_01080"
     CDS             902..1393
                     /locus_tag="VF720_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012695371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7877967.1"
                     /translation="MTLLDRLLEHDRWTTRELLLRCRELDGSALDREFDIGLKTIRAT
                     FHHVIRNMEIWSGLMDGSLTPANRAALPVDTSLPGLECRLELAADRLAVLARDVAARG
                     AWDDQWLDTFDDPPTRKSFGGAIGHVITHSMHHRAQIIHFLKRSGVADVPEGDLLGWE
                     RGS"
     gene            complement(1390..>1725)
                     /locus_tag="VF720_01085"
     CDS             complement(1390..>1725)
                     /locus_tag="VF720_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877968.1"
                     /translation="QGSDGSLYYLTYFGALRRISYPLAVSAAPETAPRPPLVAARPNP
                     FHAGGTTRFDGMESERTITIYDTQGRLVRKLDRPEWDGVDSSGRPVRAGRYFAVASSG
                     RRVGVVVTR"
BASE COUNT          270 a          587 c          598 g          270 t
ORIGIN      
        1 tcgtggagtc gggcggacgg ctcctgatgc gtcgtcgatc cgaagcggag agcctcatgc
       61 cggggatgtg ggaactcccc gcctggcgga cgcccgactc cgggtcgatc cggggccatg
      121 tcgagcgtga cctggggggc ggtagcgttg ccatcgtgcc cgcgggcgat gcccggcacg
      181 cgatcatgaa caggaggctc cgattgaacg tgcaccacgt cgagatggcg aggcggcagg
      241 cgccgccggg gtggcactgg atcgaaccgg gccggctggg cgagttgccc gtttcctcga
      301 tggttttcaa ggcgctcgaa gcggcgcgcg gcaggctcaa gctcgctttc gccctgctgc
      361 tcgcggcgag cgtggccatg gcatccggcg tgccctacgc tcacggcgaa gacccggccg
      421 tgacgccggc ggcaagcgcg gccgtgccgg cggatccgga cacgaccacg atcccccttc
      481 ccatcgtccc ggaactcgtg gtccgcgaca tcgccggcaa ggaagtctcg cttcacgccc
      541 tgcttgcgaa gggaccggtg ctcctcaatt tctgggcgct ctggtgcaag ccgtgtctca
      601 aggagatccc ggagctggtg aagctgcagg agaactacgc cgcacgggga ttcagcctca
      661 tgctggtcaa cggcgactca cccagcgacc tggcccgcgt ggcgccattc gcccgtgcca
      721 ggaaaatggc cggcccggtg gtgaccgacg tggacggcgc gctccgacgc cgcttccagg
      781 ccaacgcctt tcccacctcg atcctgctcg atcgctcggg gcgcaccgcc tggtcggccc
      841 aggggtatcg gccgggagac gaagtgaagc tggccgcgga gatcgaggca ttgctcgcga
      901 aatgaccctg ctcgaccggc tcctcgagca tgaccggtgg acaacgcggg aactgctcct
      961 gcgctgccgc gagctcgacg ggtcggcgct cgaccgcgag ttcgacatcg gcctgaagac
     1021 gatccgcgcc accttccacc atgtcattcg caacatggaa atctggtccg gcctgatgga
     1081 cgggagcctc acgccggcca atcgcgccgc gctgcccgtg gatacgagcc tccccgggct
     1141 ggaatgccgt ctcgaactgg cggcggatcg gctggctgtg ctggcccgcg acgtggccgc
     1201 gaggggagcg tgggacgatc aatggttgga caccttcgac gaccctccga cgcgaaagtc
     1261 gttcggtggc gccatcggcc acgtcatcac gcacagcatg caccatcgcg cccagatcat
     1321 tcacttcctg aagcgttccg gcgtggccga cgttcccgag ggggatctgc tcggctggga
     1381 acgcgggtcc taacgcgtca ccaccacgcc cacccgcctc ccggacgacg cgacggcaaa
     1441 gtaccgcccc gccctgaccg ggcggccgga ggaatcgact ccgtcccact cggggcggtc
     1501 cagctttcgg accaggcgcc cctgggtgtc gtagatcgtg atcgtgcgct cgctttccat
     1561 gccgtcgaac cgggtggtgc cgcccgcatg gaacggattg gggcgcgccg cgacgagcgg
     1621 aggcctgggc gccgtttcgg gcgcggcgga aaccgccagc gggtagctga tcctgcggag
     1681 agcgccgaag taggtgaggt aatagagtga gccatcgctc ccctg
//
LOCUS       DASVOD010000054         6255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_890785, whole genome shotgun sequence.
ACCESSION   DASVOD010000054 DASVOD010000000
VERSION     DASVOD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890785"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..498)
                     /locus_tag="VF720_01090"
     CDS             complement(73..498)
                     /locus_tag="VF720_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX7877969.1"
                     /translation="MSFEPHPSVVCTVIDDGGVLLHLDTKFYYSLNATALVVWDLLEA
                     GHADPDVILGDGNGRADGIRAFLDELARESLVRAAASRPAGAGNLPGGMGAANGAGAA
                     PPSPALPGGTWIAPRLTRHDIPLNQILSNPFDPSVPLAE"
     gene            719..1648
                     /gene="gnd"
                     /locus_tag="VF720_01095"
     CDS             719..1648
                     /gene="gnd"
                     /locus_tag="VF720_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEX7877970.1"
                     /translation="MTIGMIGLGRMGKNMALRLLAGGHAVVGLNRTPEPYAELTAAGG
                     VAAADLDDLVAKLPTPRGAWCMVPSGDVTEELVQSLVIKLAPGDVIVDGGNSNFHDSV
                     RRAEAAAARGIHFLDAGTSGGIWGRTVGYCLMVGGNETAFRTVEPALATLAPPDGYRH
                     VGAPGAGHYAKMVHNAIEYGMMQAYAEGFELLNVSGYDYDLETLAHLWNQGSVVRSWL
                     LELAERAFKADPALATLGDRVDDSGEGRWTLIESIDKAVPMPVLALALQARFRSRQES
                     SFSGRVLSALRREFGGHAVAPATTSPSTGEAKK"
     gene            1645..2571
                     /locus_tag="VF720_01100"
     CDS             1645..2571
                     /locus_tag="VF720_01100"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX7877971.1"
                     /translation="MTDRVREILSWYGSDNPGTKANLARMMNTGKLAGTGRMVILPVD
                     QGFEHGPARSFAVNPDGYDPRYHLRLAVESGCNAYAAPLGFLEAAAGEHAGEIPLILK
                     VNNHDVLFDEKDPLSSQTASVEDALRLGCSAIGYTIYPGSSASSMMYTELRELAEEAR
                     SVGLAVVVWSYPRGSQISKEGETAIDVVAYAAQIAAQLGAHIIKVKLPSAHIEQAEAK
                     KVYDKTAVPIGTLAERVRHVVQSSFDGRRIVIFSGGAKKDKDEDVFDEARAIRDGGGF
                     GSIIGRNSFQRPKGEAIRFLNSVMDIYGGSTR"
     gene            2568..5882
                     /locus_tag="VF720_01105"
     CDS             2568..5882
                     /locus_tag="VF720_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7877972.1"
                     /translation="MTFRKSLLAAALAVTSISGGASAETRLMRQPDIHGATIVFVYGG
                     DLWSVPRTGGSATRITTDVGIEQYPMISPDGKSIAFTAEYDGNADLFTVPITGGEPTR
                     LTYHPFTDRTVDWYPDGKSILIRSTRQSPTGRYERYFKVPAAGGFAEMLPLPTAGPAT
                     WRADGKALAYNLPSMENRTWKRYQGGSAPEVWIYDFEKNKSEKLTDWTGTDEMPLWYK
                     DTIYFNSDRTGKLNLWAYDLKSKAVRQITKYTEFDVKWPALGPDAIVFENGGRLRVMD
                     LPSEEIHEITVDVPTDRALTRAEYRNAGNAVQSASISPGAMRVAVEARGDIITVPAKK
                     GTYRPLTATPGVHERSPSWSPDGRWVACWSDASGEYEIQLLPQDGNGAPRELTKGVGG
                     YGFDLRWSPDSKSIAWIDESSRLRMVNVASGKVTDVDSDDIGDMGDFSWSYDSKWLAY
                     SKGRPNNYNVIVLYSLASGKATPVTDGMTNDVEPVFSPDGKYLAFRSTRHFDIEFNSY
                     DNEFIFPNAEGLYVLALAKDAPSPVAPESDEETPADDDKKSDDGKSGDKGDKGDKKDS
                     DGDDDADSKAAAVKVDFEGIFQRVAEVPVDHGSIFATSYLDGKLLYLVQDPEAGGGED
                     EDEEGSSGATLEYFDFDERETVTVMEGVEGYLPTPDGEKLLVRTGKGWGIIEPDEDQK
                     VKDMLAVDDIQVLIDPKAEYVQMFDEAWRLERDFFYDPRMHGVDWNEVRQRYSQLVPY
                     ATHRSDLNYLLGEMIAELNSSHAYVGGGDYPRVSRVGVGLLGADYELDAGSGRYKFKK
                     IYGQSDWIGGTMAPLGAPGVNVREGDYLIGVNGREVRAPEDVYAAFQATADRPILLRV
                     NDRPDTNGSRSYLVEPVGNEIPLRYEAWVTDNRKRVHDMTNGRVGYLHVPDTGVGGHV
                     QLSRGLYAESDKDGLIVDGRFNSGGFIPDELVQRLARRPLSYWAQRHHRAFKTPGVAI
                     GGPKVCVTNEYAGSGGDCLPYFFRELGLGPLVGKRTWGGLIGINRGINLIDGGNVTFP
                     AFSFYNLKGEWVVEDHGVDMDYEVEQIPEEVMAGHDPQLEKAIELVNDALKKSPPVTP
                     PPPVAPNRAGVRGDSR"
     gene            5960..>6255
                     /locus_tag="VF720_01110"
     CDS             5960..>6255
                     /locus_tag="VF720_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877973.1"
                     /translation="MFRIALLLSLVPPAFSRAGDVTAGGGTRSRFNLVLTDRLGSWVA
                     GHASDAPGLVLDAGLLGIDTRVTPIAWLSLSAVATREGVRLSWSITADSDPVGFL"
BASE COUNT         1094 a         2099 c         2087 g          975 t
ORIGIN      
        1 tccggccagg gcgaggctga gtagcggcgg tgacgccgcc gggtcgatcg cgtgtcgggt
       61 catggccgga tgttactcgg ccagcggcac cgagggatcg aaagggttgc tcaggatctg
      121 gttgagcggg atgtcgtgcc gggtgagtcg gggggcgatc cacgtgccgc ccggcaacgc
      181 gggtgagggt ggcgccgcgc ccgcaccgtt cgccgctccc ataccgcccg gcaggtttcc
      241 cgcacccgcc ggccgcgacg cggcggcccg caccagcgac tccctcgcga gttcgtcgag
      301 aaaggcccgg atgccatcgg cgcgtccgtt cccatcgccg aggatcacgt ccgggtcggc
      361 gtggccggct tccaggaggt cccacacgac gagggccgtg gcattcaatg aataatagaa
      421 cttcgtatcg agatggagca ggacaccgcc gtcgtcgatg acggtgcaga ccaccgaggg
      481 atgaggttcg aacgacatcg acacgactcc ggaacgttgg ggtgagtgga atgggggcga
      541 atcttccgcg atcgccccat ggcatgtcaa cgacaagccg gcaagccact tacgcggtgc
      601 cgacggatct ggacgatgcg aacgaccggg tgtaggctcc gggtccggcc aaccgcctcg
      661 cacgaggcgc ctcaatgtcg ctcgacctgc ccggccggct ctccggggga tgcctctcat
      721 gacaatcgga atgatcggcc ttggccgcat ggggaagaac atggccctgc gcctccttgc
      781 gggaggtcac gcggtcgtgg gcctgaacag gacgcccgaa ccttatgccg aactgacggc
      841 ggcggggggc gtggccgccg ccgacctcga tgatctcgtt gcgaaattac cgaccccgcg
      901 cggggcctgg tgcatggtcc cctcggggga cgtgaccgaa gagctcgtgc agtcacttgt
      961 catcaagctc gcgccgggcg atgtcatcgt ggatggaggc aactcgaact tccatgattc
     1021 cgtacgacgc gcggaagcgg cggccgcccg cggcatccat ttcctggacg ccggcacgtc
     1081 gggcggcatc tgggggcgca cggtgggcta ctgcctgatg gtcggaggca acgaaacggc
     1141 gttccggacc gtcgaaccgg cgctcgccac gctggcccca cccgacgggt atcgccatgt
     1201 cggggccccg ggcgcggggc actacgcgaa aatggtccac aacgcgatcg agtacggcat
     1261 gatgcaggcc tatgccgagg ggttcgaact gttgaacgtt tcgggctacg actacgacct
     1321 cgaaacgctc gcgcacctct ggaaccaggg gagcgtggtc cgctcgtggc tcctcgaact
     1381 ggccgaacgc gccttcaagg ccgacccggc cctggccacg ctcggcgacc gggtagacga
     1441 ctccggcgaa gggcgctgga cgctcatcga atcgatcgac aaggcggtgc cgatgccggt
     1501 cctcgcgctg gcattgcagg cccgatttcg ttcccggcag gaatcgtcct tctcgggacg
     1561 cgtcctgtcg gccctccgac gcgaattcgg cggccacgcc gtcgcgccgg cgacgacatc
     1621 cccctccaca ggagaagcca agaaatgacc gaccgcgttc gcgagatcct gtcctggtac
     1681 gggagcgaca acccgggcac gaaggccaac ctggcccgga tgatgaatac cggcaagctt
     1741 gccggcacgg gcaggatggt gatcctcccg gtggaccagg ggttcgagca cggtcccgcc
     1801 cgctcattcg cggtgaatcc ggacggatac gacccgcgtt accacctgcg cctggcggtc
     1861 gagtcggggt gcaatgccta cgcggcgccg ctcggtttcc tcgaggcggc cgcgggcgag
     1921 catgccggtg aaatccccct gatcctcaag gtcaacaacc acgacgtgct gttcgacgag
     1981 aaggaccccc tctccagcca gacggccagt gtcgaggatg cgctccggct cggctgctcc
     2041 gccatcggct acacgatcta tcccggctcg tccgccagtt cgatgatgta caccgagctg
     2101 cgcgagctgg ccgaggaggc ccggagcgtc ggcctcgcgg tggtggtctg gtcgtacccg
     2161 cgcggcagcc agatctccaa ggaaggggag acggccatcg acgtggtggc ctacgccgcg
     2221 cagatcgccg cccagctcgg cgcgcacatc atcaaggtca agctgccgag cgcgcacatc
     2281 gagcaggccg aggccaagaa ggtgtacgac aagacagcgg tgccgatcgg cactctggcc
     2341 gagcgcgtgc gccacgtcgt ccagtcgtcg ttcgacggcc ggcgcatcgt catcttctcg
     2401 ggcggcgcga agaaggacaa ggacgaggat gtgttcgacg aggcgcgcgc catccgcgac
     2461 ggcggcggct tcggctcgat catcggccgc aattcgttcc agcgcccgaa gggcgaggcc
     2521 atcaggttcc tgaattcggt catggacatt tacgggggtt cgactcgatg acgttccgca
     2581 aatccctcct ggcggccgca ctcgctgtca cttcgatctc cgggggagcc tcggccgaga
     2641 cgcgtctcat gcgccagccg gacatccatg gcgccaccat cgtcttcgtt tacggcggcg
     2701 acctctggtc ggtgccccga acgggcggtt cggccacgcg gatcacgacc gacgtcggca
     2761 tcgagcagta cccgatgatt tctcccgacg ggaagtcgat cgccttcacg gccgagtacg
     2821 acggcaacgc cgacctcttc accgtgccga tcaccggtgg cgagcccacc cggctcacct
     2881 accacccgtt cacggaccgc accgtcgact ggtatcccga tggcaagtcg atcctcatcc
     2941 gctccactcg acagagcccg accggtcgct acgagcgcta cttcaaggtg cccgcggcgg
     3001 gcggattcgc ggagatgctg ccgctcccca ccgcgggccc cgccacttgg cgggccgacg
     3061 ggaaggcgct ggcctacaac cttccgtcga tggaaaaccg cacctggaag cgctaccagg
     3121 ggggcagcgc ccccgaggtc tggatctacg acttcgagaa aaacaagagc gagaagctca
     3181 ccgactggac cggcaccgat gaaatgccgc tctggtacaa ggacaccatc tacttcaact
     3241 ccgatcgcac cggcaagctc aacctctggg cctacgatct caagtcgaag gcggtccgcc
     3301 agatcacgaa gtacaccgag ttcgacgtga agtggccggc gctcggtccc gatgccatcg
     3361 tcttcgagaa cggcgggcgg ctccgcgtga tggacctccc atccgaggag atccacgaga
     3421 tcacggtgga cgtgccgacc gatcgggccc tgacccgcgc cgagtaccgc aacgccggca
     3481 acgcggtgca gtcggcctcg atttccccgg gcgcgatgcg cgtggcggtc gaggcccgcg
     3541 gcgacatcat cacggtgccg gccaagaagg ggacgtaccg gccgctgacg gccacgcccg
     3601 gcgtgcacga acgctctccc tcctggtcgc ccgacggccg ctgggtggcc tgctggtcgg
     3661 acgcctccgg cgaatacgag atccagctcc tgccgcagga cggcaacggc gcgccgcgtg
     3721 agctcacgaa gggagtgggc ggatacggct tcgaccttcg ctggtccccc gactcgaagt
     3781 cgatcgcctg gatcgacgag tcgtcgcgcc ttcggatggt gaacgtggcc tcgggaaagg
     3841 tgaccgatgt cgattcggac gacatcggcg acatggggga cttctcctgg tcctacgatt
     3901 cgaagtggct ggcctacagc aaggggcggc cgaacaacta caacgtgatc gtcctctaca
     3961 gcctggccag cggaaaggcc acgccggtca ccgacggcat gaccaacgac gtcgagccgg
     4021 tcttcagccc cgacggcaag tacctggcct tccgctccac gcgccacttc gacatcgagt
     4081 tcaactcgta cgacaacgag ttcatcttcc cgaatgccga ggggctttac gtcctggctc
     4141 tcgcgaagga cgccccctcg ccggtggcgc ccgagagcga cgaggagacg ccggccgacg
     4201 acgacaagaa gtcggacgac gggaaatccg gcgacaaggg ggacaagggc gacaagaagg
     4261 attccgacgg cgacgacgac gccgattcga aggccgcggc cgtcaaggtc gacttcgagg
     4321 gtatcttcca gcgcgtcgcc gaagtgccgg tggaccacgg ttcgatcttc gccaccagct
     4381 acctcgacgg caagctcctc tacctggtcc aggatcccga agcgggtggg ggagaagacg
     4441 aagacgagga agggtcatcc ggcgccacgc tcgagtactt cgacttcgac gagcgggaga
     4501 cggtaaccgt gatggaaggc gtggaggggt acctgcccac gcccgacggc gagaagctcc
     4561 tcgtccgcac cggcaagggg tgggggatca tcgagcccga cgaggaccag aaggtcaagg
     4621 acatgctggc ggtcgacgac atccaggtgc tgatcgatcc caaggccgag tatgtccaga
     4681 tgttcgacga ggcctggcgc ctcgagcgcg acttcttcta cgaccccagg atgcacggcg
     4741 tggactggaa cgaggtgcgc cagcgctaca gccagctcgt gccctacgcc acgcaccgga
     4801 gcgacttgaa ctacctcctc ggcgaaatga tcgccgagct caattccagc cacgcctacg
     4861 tgggtggcgg cgattacccg cgcgtgagcc gcgtgggcgt ggggctgctc ggcgccgact
     4921 acgaactgga tgccggcagc ggccgctaca agttcaagaa gatctacggt caaagcgact
     4981 ggatcggcgg cacgatggcg ccgctcggcg cgccgggcgt caacgtcagg gaaggtgact
     5041 atctcatcgg cgtgaatggt cgcgaggttc gggcgccgga ggatgtgtac gccgccttcc
     5101 aggccacggc cgatcgaccg atcctcctcc gcgtgaacga tcgcccggat accaacggct
     5161 cgcgcagcta cctcgtcgag ccggtgggga acgaaatccc actgcgctac gaggcgtggg
     5221 tcaccgacaa tcgcaagcgc gtccacgaca tgacgaatgg tcgcgtggga tacctccacg
     5281 tgcccgacac gggcgtgggt ggacacgtgc agttgagccg cggcctctac gccgagtcgg
     5341 acaaggacgg gctgatcgtc gacgggcgat tcaactcggg cggcttcatc cccgacgagc
     5401 tggtgcagcg cctggcccgg cgccccctgt cgtactgggc gcagcgtcat caccgggcct
     5461 tcaagacgcc cggcgtggcc atcggcggac caaaagtctg cgtcaccaac gagtacgccg
     5521 gctcgggcgg cgactgcctc ccgtacttct tccgcgagct cggcctcggg cccctggtcg
     5581 gcaagcgcac ctggggcggt ctcatcggca tcaaccgcgg catcaacctg atcgacggcg
     5641 gcaacgtcac cttcccggcg ttcagcttct acaacctgaa gggtgagtgg gttgtcgagg
     5701 accacggcgt cgacatggac tacgaggtcg agcagatccc cgaagaggtg atggccggcc
     5761 acgacccgca actcgagaag gccatcgaac tggtcaacga cgcgttgaag aagagcccgc
     5821 ctgtcactcc gccgccgccg gtcgcgccca accgcgccgg cgtgaggggc gactcgcggt
     5881 gacgtcgttt cgaccggctg ccgtgagggt ctgcgccctc gcggcagccg ggcgctgttt
     5941 ttccgttggc tctgcgcgct tgtttcgcat tgccctgttg ctgtccctgg tcccgccggc
     6001 cttcagccgg gcgggcgatg tgaccgccgg cggcggcact cgctcgcgtt tcaacctggt
     6061 cctcaccgat cgtctcggct cgtgggtggc cgggcacgcg agcgatgcgc caggcctcgt
     6121 cctcgacgcc ggtctgctcg gcatcgacac ccgtgtcacg cccatcgcgt ggctcagcct
     6181 gtccgccgtg gccacccgcg agggcgtgcg cctgtcgtgg tcgatcactg ccgactcgga
     6241 tccggtcggg tttct
//
LOCUS       DASVOD010000055         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_369183, whole genome shotgun sequence.
ACCESSION   DASVOD010000055 DASVOD010000000
VERSION     DASVOD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369183"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VF720_01115"
     CDS             <1..935
                     /locus_tag="VF720_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877974.1"
                     /translation="SDNGILVFSTRRAQRGRLVRYDRTGRELGVLNTPASAFHPALSP
                     DGRRLVFRAMDEVSRSRDLWLCDVTRDVSTRFSYEPANENYPVWAPDGRAVFYYSDAA
                     GGVGIYRKEVTGAGLSTQVAASPSGECTISGVSSDGRHLLFDAVGSEGRQDIYVLDLS
                     VSPGKPAVFLNGPFDEWHGTFSPDGRYMAYTSLESGRNEVYVQSYPDRADKWQVSIRG
                     GNEPQWSADGRQLYYLSSEQVIMSVPAGVAGHFEPGTPERLFATAVLQPDGPRPHYAV
                     APDGETFYVMAPASTQSLPSTYVVINWQQAFRNP"
     gene            complement(942..>2688)
                     /locus_tag="VF720_01120"
     CDS             complement(942..>2688)
                     /locus_tag="VF720_01120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7877975.1"
                     /translation="TFRDPYPIVIDVQVAPDRKHADVEAAIKATLERVALEGVTAAEM
                     ERARKQIEVAVIRGRDGTYPLCSSLGEAVASADWKWFVTYIDRMKGVSAADVKRVAAT
                     YLIPDRATVGWFVPANAEQVAPLKAAAEAMVVPAGSGATDSKAPSTNMGSATTTNSGT
                     FASRTTHKVLSNGIVLDIVENHAVPTVAISGILLAGNVTAPKETPGVATLTAMMLDRG
                     TTTRDKKTIGDQLAKAGANLFIGAGPLEMNITGNGMSRDIDLLLDILADQLKNPAFPD
                     SEFTKVKAEYQSDVMEAAENTGFRATEQLSRLVYPEGHPYRVPEHTVFLKALETTTLD
                     NVRAFHKRRIVGSALKLAIVGDVAAADVAAKVEAYFAGLPDGTPPVMEAPRTSTNAVQ
                     KTVVTLPGKANMNFVAGHASGLRRTDPDFEAALIANAALGQNSLSSRIGKRVRDTEGL
                     SYNLSSRYSYTDALDGMWLVNVNVAPQNLSKAMASTMDVIRQYAKEGITEEEVAIGKS
                     YFAGNYQVRLGSNAGVARALVEAEKFGFGPGYLDEYPERFRRVTKGQVDAALRKHLDP
                     AKLHTVVAGDLKTVP"
BASE COUNT          393 a          907 c          903 g          485 t
ORIGIN      
        1 cctcggacaa cggtatcctg gtcttttcca cgcgccgggc gcaacggggg aggctggtgc
       61 gttatgaccg gaccggccgg gaactcggcg tgctgaatac cccggcttcg gcgtttcatc
      121 ccgccctgtc gcccgacggg cggcgcctcg tgtttcgtgc catggacgaa gtgtcccgga
      181 gccgggacct ctggctgtgc gacgtgaccc gcgatgtctc gacccgattc agctacgaac
      241 ccgccaacga gaattacccg gtgtgggctc cggacggacg cgcggtcttc tattactcgg
      301 acgccgcggg cggcgtgggg atctaccgga aagaagtgac cggcgccgga ctgagcaccc
      361 aggtggccgc ctcaccgtcg ggcgaatgca ccatctccgg cgtctcgagc gatggtcgcc
      421 atctcctctt cgacgcggtc gggtcggaag gacgacagga catctacgtg ctcgacctgt
      481 ccgtctcccc gggcaagccc gcggtcttcc tgaacgggcc gttcgacgaa tggcacggga
      541 ccttctcacc cgacggacgc tacatggcct atacctcgct cgaatcggga cggaacgagg
      601 tgtacgtcca gtcgtacccg gatcgcgcgg acaagtggca ggtgtcgatc cgcggcggca
      661 acgagccgca gtggagcgcc gacggacgtc agctttacta cctgtcctcc gagcaggtca
      721 tcatgtccgt gcccgcgggg gtggccggac atttcgagcc gggaacgccg gagcgcctct
      781 ttgccacggc ggtgctgcag cccgacgggc cgcgccccca ctacgcggtg gcgccggatg
      841 gcgagacgtt ctacgtcatg gccccggcct cgacccagtc gcttccaagc acctacgtcg
      901 tgatcaactg gcaacaggcg ttccggaatc cctgacgccc gtcagggaac cgtcttcagg
      961 tctcccgcca cgaccgtgtg cagcttcgcg gggtcgaggt gcttcctgag ggccgcatcc
     1021 acctgaccct tcgtcacccg ccggaagcgc tcggggtact catcgaggta gcccggcccg
     1081 aagccgaact tctcggcctc gaccagggcg cgcgccacgc cggcgttgga gccgaggcgc
     1141 acctgatagt tgccggcgaa gtagctcttg ccgatcgcca cttcctcctc ggtgattcct
     1201 tccttcgcgt actggcggat gacgtccatg gtggaggcca tcgccttcga gaggttctgc
     1261 ggggccacgt tgacgttgac cagccacatc ccgtcgagcg catcggtgta gctgtagcgt
     1321 gacgacaggt tgtacgacag cccctccgtg tcacgcaccc ttttcccgat gcgcgaggag
     1381 agggagttct ggccgagggc ggcgttggcg atgagcgccg cctcgaagtc gggatcggtg
     1441 cggcgaagtc ccgaggcatg tccggccacg aagttcatgt tggccttccc cggcagggtg
     1501 acgaccgtct tctggaccgc gttggtggag gtgcgcggcg cctccatgac cgggggcgtt
     1561 ccgtccggca ggccggcgaa gtaggcctcg accttggccg ccacgtcggc ggccgcaaca
     1621 tcgcccacga tggccagctt gagcgcgctc ccgacgatgc ggcgcttgtg gaacgcgcgc
     1681 acgttatcca gcgtggtcgt ctcgagcgcc ttcaggaaca cggtatgctc gggcacgcgg
     1741 taggggtgcc cctcggggta caccagccgc gagagctgct cggtggcccg gaagccggtg
     1801 ttctcggccg cctccatcac gtcggactgg tattcggcct tcaccttcgt gaactcggaa
     1861 tcgggaaacg ccgggttctt gagctggtcg gccaggatgt cgagcaacag gtcgatgtcg
     1921 cgcgacatgc cgttgccggt gatgttcatc tcgagcggcc ccgcaccgat gaagaggttg
     1981 gcgccggcct tggccagctg gtcgccgatc gtcttcttgt cgcgcgtcgt cgtgccgcgg
     2041 tcgagcatca tggccgtgag cgtggccacg cccggcgtct ccttcggggc ggtcacgttg
     2101 ccggccagca ggatgccgga gatggccacc gtcggcaccg cgtggttctc cacgatgtcg
     2161 aggacgatgc cgttcgacag caccttgtgc gtggtgcggg aggcgaaggt gccggaattg
     2221 gtggttgtcg cggagcccat gttcgttgac ggagccttgc tgtccgtggc cccgctcccg
     2281 gcgggaacga ccatggcttc ggccgccgcc ttgagcggcg ccacctgctc ggcgttggcc
     2341 ggcacgaacc atcccaccgt ggcccggtcg ggaatgaggt aggtggcggc caccctcttc
     2401 acgtcggccg cgctcacccc cttcatccgg tcgatgtagg tcacgaacca cttccagtcg
     2461 gcggaggcca cggcctcacc caggctcgag cagaggggat aggtgccgtc gcgcccgcgg
     2521 atcaccgcca cttcgatctg cttgcgggcc cgctccatct ccgcggcggt cactccctcg
     2581 agcgcgaccc tttccagcgt ggccttgatg gccgcttcca cgtccgcgtg cttcctgtcg
     2641 ggcgccacct ggacatcgat cacgatcggg tagggatcac ggaacgtg
//
LOCUS       DASVOD010000056         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_264827, whole genome shotgun sequence.
ACCESSION   DASVOD010000056 DASVOD010000000
VERSION     DASVOD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264827"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VF720_01125"
     CDS             <1..569
                     /locus_tag="VF720_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005543015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX7877976.1"
                     /translation="MELAGRYAYAGRDWVCEEVARLLGARILEEVHNHHNFAWRERHD
                     GRDLWVVRKGATPAFPGQKGFVGGSMGDISVILEGVESEESKQALYSTVHGAGRVMSR
                     TAARGKLNYRTRQVVTPGAVSREMMMDWVRKAGVELRGAGTDESPHCYKRLPEVLRHH
                     AASVRILHTLQPIGVAMAGEDVVDPYKD"
     gene            584..865
                     /locus_tag="VF720_01130"
     CDS             584..865
                     /locus_tag="VF720_01130"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEX7877977.1"
                     /translation="MSSRARPAVFPNLKPSTETISLRLPASLLAELKSMANEREISSP
                     LCEYRRKKETTGGPKGSSAPFERLTLEASDMIYSRYCFFRRTCMEAQRP"
     gene            862..1263
                     /locus_tag="VF720_01135"
     CDS             862..1263
                     /locus_tag="VF720_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HEX7877978.1"
                     /translation="MIARGPFDVKLTPQSPTGDNPEPTLGRMLLVKSFHGDLEATSTG
                     EMLTAGTSTKGSAAYVAVERVTGTLHGRRGTFSLHHTGIMTRGVPDLSIHVVPDSGDG
                     ELAGISGTLAIQIEGGKHSYELTYDLPGADG"
     gene            1275..>1568
                     /locus_tag="VF720_01140"
     CDS             1275..>1568
                     /locus_tag="VF720_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX7877979.1"
                     /translation="MKSAVPDTHYPPLIGAQDRVILFDGVCNLCNGTVQFILQHDRKR
                     VFRLAAIQSPAGQALMDWFGFMPGSLATLILVEGNRASTRSTAVLRIANQLPWP"
BASE COUNT          293 a          492 c          519 g          264 t
ORIGIN      
        1 gcatggagct ggccggccgc tacgcttacg cggggcgcga ctgggtgtgc gaagaggttg
       61 cgcgcctcct cggcgcgcgc attctcgaag aggtgcacaa ccaccacaat tttgcctggc
      121 gcgaacgcca tgacggacgc gatctgtggg tggtacggaa gggggccacg ccggcgttcc
      181 ccgggcagaa gggttttgtc ggcggctcga tgggggacat ctccgtgatc ctcgaaggag
      241 tggaatcgga ggagtcgaag caggcgcttt attccacggt gcatggcgcg gggcgtgtga
      301 tgagccgcac cgcggcccgt ggcaaactga actatcgtac gcgccaggtg gttacgcccg
      361 gcgccgtctc gcgggagatg atgatggatt gggtcaggaa ggcgggcgtc gagctccgcg
      421 gcgccggcac cgacgagtca cctcattgct acaagcggct gccggaagtg ctgcgacacc
      481 atgccgcctc cgtccgcatc cttcatactc tccagccgat cggcgtggca atggcggggg
      541 aggatgtggt ggatccgtat aaggactaaa tgacgcttgc gggatcagta gccgggctcg
      601 tccggcagtt ttcccgaacc tgaagccgtc gacggagaca atttctcttc gcttgccggc
      661 aagcctcctg gcggagctca agtcgatggc gaacgagagg gagatctcga gtccgctatg
      721 cgagtaccgg cgcaaaaagg aaacgaccgg cggtccgaaa ggcagctcag cgccgttcga
      781 acggctaacg ctcgaagcat cggatatgat ctattccagg tattgcttct ttcgcagaac
      841 ctgcatggag gcacaacgcc catgatcgcc cgaggcccgt tcgacgtgaa actcacccct
      901 caatccccga ccggcgacaa ccccgaaccg acactcggcc ggatgctgct cgtgaagtca
      961 ttccacggag atctcgaagc caccagcacc ggcgagatgc tgaccgccgg gacgagcacc
     1021 aaaggctcgg cggcatacgt ggcggtcgag cgcgtcaccg gcacgctgca cgggcggagg
     1081 ggcaccttct cgctgcacca caccggcatc atgacgcggg gcgtgccgga tctcagcatc
     1141 catgtcgtgc ccgactcggg tgacggcgag ctggccggca tctcgggcac gctggccatc
     1201 cagatcgagg gggggaaaca ttcgtacgaa ctgacgtatg acctgccggg cgcggacggc
     1261 tgatcgagga ctctgtgaag tcagcggttc cggatacgca ctacccgccg ctcatcgggg
     1321 cccaggaccg ggtcatcctc ttcgacggcg tctgcaatct ctgcaacggc acggtccagt
     1381 tcatccttca acacgatcgc aagcgggtgt ttcgcctcgc ggccatccag tcgccggcag
     1441 gacaggccct gatggactgg ttcggcttca tgcccgggag cctggccacg ctgatcctcg
     1501 tggaaggaaa ccgcgcctcg acgcgctcga cggcggtgct gcggatcgcc aaccaactgc
     1561 cctggccg
//
LOCUS       DASVOD010000057         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_369382, whole genome shotgun sequence.
ACCESSION   DASVOD010000057 DASVOD010000000
VERSION     DASVOD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369382"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..>1582
                     /locus_tag="VF720_01145"
     CDS             432..>1582
                     /locus_tag="VF720_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7877980.1"
                     /translation="MLLADPAVVSVAEDRVVPLAACTPSDPYFDHASPDSAQWSLHGN
                     PAGPDLGALDAWNVTTGAPGAILAILDSGTDWHHPEFGPPLSPEGRIWFNPEEVNGTP
                     GVDDDDNGFIDDVRGWDFVDVSTIPGEVNRVDPAEDGLVPDNDPVDYDGHGTFVSGIA
                     GAAMDDGVGMAGVAPGVPIMPLRVGWREDYVTEGGRRVVGGVVSMSFCAQAIVYAADN
                     GARVLNCSWLSVQTPEIEAALNYAIGVKGVVVVDAAGNGGLIETQSQNYLSRRGDCIE
                     VAALAKSGELSGVTSIGSWVDISAPGENLLSLVKTGASRLYRINETGATSFAAPFVSA
                     TAALVRSVAPGMTPAEVRMVLMANVTDIYGIGVNSFYAGKLGAGLVNAG"
BASE COUNT          240 a          526 c          537 g          279 t
ORIGIN      
        1 tgcggatgtc gaggtaatag ttgctttcgg cgccggaagc caggatgaag tggccccgcc
       61 ggagcgcctt ttcgagtatc agcgacttga gtcgcgcgcg attcgtgtcg ttgtcaggca
      121 tgacgggacg gtacggagcg cgtcgaccat cgtcaaggag ggcgctggcg cgcccgatcg
      181 cgtgctatcg ttcccgcatg tcgtccgttc gatcgtctgc gctcgccacc gccctccttg
      241 tcgtcccgac agtcgtccag gcgcgcccga cggaatcccg tatcgttcag ctggcgcctg
      301 gggcgcaacc gccatcggtt gccggccgtt gggagcccct ctttcccggg cacgaaaccg
      361 ccggcgatcc gctctccgta ttttttcgat ggattccggg agacgaccgg cctcttgcct
      421 cggcgagcgc cgtgctcctc gcggatccgg cggtcgtctc cgtggcggaa gaccgcgtcg
      481 tcccgctggc cgcgtgcaca ccttccgatc cgtacttcga ccatgcctcg cccgactcgg
      541 cccaatggtc gctccacggc aacccggccg gcccggacct cggggcgctc gatgcctgga
      601 acgtcacgac gggcgctcct ggcgcgatcc tggccatcct cgacagcggc accgactggc
      661 atcacccgga attcggaccg ccgctctcgc cggaggggcg gatctggttc aatccggagg
      721 aagtgaacgg aacaccgggg gtcgacgatg atgacaacgg cttcatcgac gacgtgcgcg
      781 gctgggactt cgtggatgtc agcacgatcc cgggagaggt gaaccgcgtc gacccggccg
      841 aggacgggct cgtccccgac aacgatccgg tcgactatga cggccacggg acgttcgtct
      901 ccggcatcgc cggcgcggcg atggacgacg gggtgggaat ggccggcgtg gcgcccggcg
      961 tgccgatcat gccgctccgc gtggggtggc gcgaggacta cgtcaccgag ggaggtcgcc
     1021 gcgtcgtcgg gggagtcgtc tcgatgagct tctgcgccca agccatcgtg tatgccgccg
     1081 acaacggtgc gcgggtcctc aattgctcgt ggctctccgt ccagacgccc gaaatcgagg
     1141 cggctctgaa ctacgccatc ggggtgaagg gagtcgtggt ggtcgacgcc gccggcaacg
     1201 gcgggctcat cgaaacgcaa tcccagaatt acctgagccg gcgcggcgac tgcatcgagg
     1261 tggctgccct cgccaaatcg ggggagctgt ctggcgtcac ctcgatcggc agctgggtcg
     1321 acatttccgc gccgggcgag aatctcctgt cgctggtcaa gaccggcgct tcacggcttt
     1381 atcgcatcaa cgagaccggc gccacatcgt ttgccgcgcc attcgtttcc gcaacggccg
     1441 ccctggtccg atcggtcgct ccggggatga cgccggccga ggtacgcatg gtcctgatgg
     1501 ccaacgtcac cgacatctac ggcatcggcg tgaactcgtt ctacgccgga aagctgggtg
     1561 cgggattggt gaacgcgggc ag
//
LOCUS       DASVOD010000058         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_838927, whole genome shotgun sequence.
ACCESSION   DASVOD010000058 DASVOD010000000
VERSION     DASVOD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838927"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VF720_01150"
     CDS             <1..1269
                     /locus_tag="VF720_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX7877981.1"
                     /translation="QRITLLESPGFTKPTTTGFWSEDLPPQRTFTEVLDGKRYHVTEI
                     RTALFATSAGEFTIGPAILECVVPQVQRVVDNDPFSMFGRSMFGSQKVTLKTKELKIN
                     VKPLPPGAPPEFAGAVGDFRLTTQADRTTAAQGDPVTLTVTVSGSGNIKTVPDPKVPS
                     MPEFKAYDSSSSVDVNNTGELVKGTRTSQIVLVPLKAGAMTLPPITMAWFDPKAGAYR
                     TDRSSPMALTITPGAVTAGGALGGGGRGNIEVVGQDVRFLRTTLGSVSHAEHRAWDSP
                     LFWLLQILPIGVMVGAVVVERRRRRFNADIGFARQHRSGREANRRLKTARALAAKNDD
                     GLFAELAAAVSGYVADRLNRSAAGLTHEEMRSRLMVRGIRTELIDTLIRFLEACDMAR
                     FAPAATGAPERQRLLDEAGALIENLKRGGL"
     gene            1269..2069
                     /locus_tag="VF720_01155"
     CDS             1269..2069
                     /locus_tag="VF720_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877982.1"
                     /translation="MGLWARAGMGVARGAVLLLALISLVPTSAHARRPNQAEQARFAR
                     GNSAYESGDFAAAVAAYEELAATGVVGRDLYYNLGNANLKLGHLGPAILNYRRALRVD
                     SRDADARANLDYARRRTQDARPLDLPDPLPWLTAIRPGSDRSAWLYLLALNLAALAFA
                     ALRLLRDPPAALKPVVALGLTAAALFGFIFFWELRVESGRREGVVMSPVAEVRTGPGD
                     TYTVAFQVHEGTELDLGRAANGWLEVSVASPTNELKGWVAPGAVEAIP"
     gene            2075..2902
                     /locus_tag="VF720_01160"
     CDS             2075..2902
                     /locus_tag="VF720_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877983.1"
                     /translation="MTHHDPTPAGRHARRRPAPWVWLLALPLALLLVVAAVFLFVPVD
                     SLRGTVERQFSRATGYQVTIGRLNLHLVGMGLGLAAGDFRAISRDGSQSITVPELGMG
                     VRLIPLFSRRVEITLVEAPDLEFRMGAAGSMGKAAATPPAAQEHGASSTLEAPDIRIH
                     KGRIVHAGTNGITRISDITLQGGFRTTATGGAFEGDVRADSLSFAPAASPNAPLTLPR
                     FEARFHSDIDTRAVSASSTLEGKVGTIPARGSVESVSTGGHWGTRGTSHSSRSASTT"
BASE COUNT          446 a         1064 c         1013 g          456 t
ORIGIN      
        1 cagcggatca cgctgctcga atcgcccggc ttcaccaagc ccacgaccac cggcttctgg
       61 tcggaggacc tgccgcccca gcgcaccttc acggaggtgc tcgacggcaa gcgctaccat
      121 gtcaccgaga tccggaccgc cctcttcgcc acctcggccg gggagttcac gatcgggccg
      181 gccatcctgg agtgcgtggt gccgcaggtg cagcgcgtgg tggacaacga cccgttcagc
      241 atgttcggcc gctcgatgtt cggcagccag aaggtgacgc tcaagacgaa ggaactgaaa
      301 atcaacgtga agccgctgcc gccgggcgcg ccgccggagt tcgccggcgc ggtgggcgac
      361 ttccgcctga cgacgcaggc cgaccggacg accgccgccc agggagatcc ggtcacgctc
      421 acggtgacgg tgtccggctc gggcaacatc aagacggtgc ccgatcccaa agtgccttcg
      481 atgccggaat tcaaggcgta cgattcgtcg tcctcggtcg acgtcaacaa caccggcgaa
      541 ctcgtgaagg gaacgcgcac cagccagatc gtgctggtgc cgctcaaggc cggggcgatg
      601 acgcttcccc cgatcaccat ggcctggttt gacccgaagg ccggcgccta caggacggat
      661 cgctcgagcc cgatggcgct cacgatcacc cccggcgccg tcacggccgg tggcgcgctg
      721 ggcgggggag ggcgcggcaa catcgaggtg gtcgggcagg acgtccgctt cctgcgcacc
      781 acgctcgggt cggtgagcca cgcggaacac cgcgcctggg attccccgtt gttctggctg
      841 cttcagatcc tgccgatcgg tgtcatggtg ggggccgtgg tcgtggagcg ccgccgccgt
      901 cgcttcaacg ccgacatcgg ctttgcccgc cagcaccgct cgggccgcga agccaaccgg
      961 cgcctcaaga cggcccgcgc gctggccgcg aagaacgatg acggcctctt cgcggagctg
     1021 gccgcagccg tatcaggtta cgtggcggac cggctgaatc gatccgcggc cggtctcacc
     1081 cacgaggaaa tgcgctcgcg cctcatggtg cgcggcatca ggacggaact catcgacacg
     1141 ctcatccggt ttctcgaagc ctgcgacatg gcgcgcttcg cgcccgcggc caccggtgcg
     1201 ccggagcggc agcgcctgct ggatgaagcc ggtgcgctca tcgaaaacct gaagcgaggg
     1261 ggattgtgat ggggctctgg gcgcgcgcag gcatgggtgt cgcgcggggc gcggtactgc
     1321 tcctggcgct gatctccctc gtcccaacgt ccgcgcacgc gcgtcgcccg aaccaggccg
     1381 agcaggcgcg cttcgcccgg ggcaactcgg cctatgaatc gggcgacttc gcggcggccg
     1441 tggcggctta cgaggagctg gcggccaccg gcgtcgtcgg gcgcgatctc tactacaacc
     1501 tcggcaacgc caacctcaag ctgggccacc tgggcccggc catcctgaac taccgacgcg
     1561 cgctccgtgt cgattcgcgc gacgccgatg cccgcgccaa cctcgactac gcgcgccgcc
     1621 gcacgcagga cgcccgtccg ctcgacctcc ccgatccgct cccgtggctc acggccatcc
     1681 ggccgggctc ggatcgctcg gcgtggctct atctcctcgc gctcaacctc gcggccctgg
     1741 ctttcgcggc gctgcggttg ctccgcgatc cgcccgccgc gctcaagccg gtcgtggcgc
     1801 tcggcctcac cgcggccgcg ctcttcggct tcatcttctt ctgggagctg cgcgtcgaat
     1861 cgggtcgccg ggaaggggtg gtcatgtcgc cggtggccga ggtacggacc ggtccgggcg
     1921 atacctacac cgtggccttc caggtgcatg aaggaacgga actcgacctc gggcgcgccg
     1981 ccaacgggtg gctggaagtg agtgtggcca gcccgaccaa cgagctcaag gggtgggtgg
     2041 cgccgggggc cgtcgaagcg ataccctgaa ggcaatgaca caccacgacc ccacgccggc
     2101 cggccgccac gcgcggcgcc gcccggcccc gtgggtgtgg ctcctcgccc tgccgctggc
     2161 cctcctcctc gtggttgctg cggtcttcct cttcgtcccg gtggattccc tgcgcggcac
     2221 cgtcgagcgc cagttcagcc gcgccaccgg ctaccaggtg acgatcggtc ggctcaacct
     2281 ccacctcgtg ggcatggggc ttggcctggc ggcgggagat ttccgggcga tctcacggga
     2341 tggctcgcag agcatcacgg tccccgaact cggcatgggg gttcgcctga taccgctctt
     2401 ctcgcggcgg gtggagatca cgctggtgga ggcgcccgac ctggagttcc gcatgggggc
     2461 tgccggatcg atggggaagg cggccgccac gccacccgcg gcccaggagc atggggcttc
     2521 ttccacgctc gaggcgcccg acatccgcat ccacaagggg cgaatcgtcc acgccggcac
     2581 gaacggcatc acccggattt ccgacatcac gctgcagggt ggtttccgca cgaccgccac
     2641 cggcggtgcg ttcgaagggg atgtccgggc cgacagcctc tcgttcgcgc cggccgcttc
     2701 acccaacgcg ccgctcacac tgccccggtt cgaggcgcgc ttccactcgg acatcgacac
     2761 gcgcgccgtg tcggcgtcgt cgacgctcga ggggaaggtc ggcaccatcc cggcccgtgg
     2821 ttcggtggaa tcggtgtcga ccggaggtca ctggggaaca aggggcacat cacactcgag
     2881 ccgatcggcc tcgacgacgt gaagccgctg ctatcggggc aactggccgt cacgatctcg
     2941 cagtacaagc tcgacggccg cctcgagcgg ggagagatc
//
LOCUS       DASVOD010000059         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_212799, whole genome shotgun sequence.
ACCESSION   DASVOD010000059 DASVOD010000000
VERSION     DASVOD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212799"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="VF720_01165"
     CDS             <1..1547
                     /locus_tag="VF720_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877984.1"
                     /translation="MAPPTKRPEVLEEKAREMLARIELPKSTGLAAQGFIDDNSLLRW
                     MKNRDRTRARWSRLETGEAPALLYWYRELHPRDTATRFGSIGRISRTDPPFEQAGMSS
                     VLLDGAGKLRSISVIPPRADTLAAAAEPEWAPWFGLAGLDTARFHPVEPERNPSFYVD
                     GRRAWRGTLASSPADSYLVEGATYRGRLARFAVITPWDPGDRFNDAVSAGQRTAQSVV
                     SFLIIMTVLVGGFLITRRNLRLERADRLGAGRVALAVGGSLMLTWLFTASHTGDLASE
                     IGVFWNGMAIACLNGLLAWLIYIGVEPYVRRAKPHLLIGWTRFLAGGFRDPIVGRDVL
                     IGAVAGVTLHVLQGIGWLVPTWLGQPPETPLSIDPDTLLGFGTMLGAILASLTNVVTT
                     PLVVLIIYVIGRMVFRKEWAALGLVALLFGGAALTGQTSTVRLVIGLIYTAVLLLVVF
                     RWGFLAAGSAILVEGLVSNYPFAMNPSTWYFPTTLAVLSLVALLGYWAWRAARSRLML
                     ARPTTR"
     gene            complement(1534..>1964)
                     /locus_tag="VF720_01170"
     CDS             complement(1534..>1964)
                     /locus_tag="VF720_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877985.1"
                     /translation="EATTGHYLPEFGVLFTAEVSLLNRHWWYGKKANWSFGWDDDDDD
                     DEDYDDDSAKMLARQEKQYAQFKDEMMEALMAGGDNFSGRLSDTQWVGVSVELSDSRY
                     FKKHKLHQLQMKARVSDLKSYAAGSLNEEGMKSKIVVTES"
BASE COUNT          267 a          675 c          656 g          366 t
ORIGIN      
        1 agatggctcc gccgaccaag cgtcccgagg ttctcgagga gaaagcgcgg gagatgctgg
       61 cgcgaatcga gctgccgaaa tccaccggtc tcgccgcgca gggcttcatc gacgacaact
      121 ccctgctgcg gtggatgaag aatcgcgacc ggacccgggc ccgctggtca cgactcgaga
      181 cgggcgaggc gccggcgctc ctttactggt accgcgagct ccacccgcgc gacacggcca
      241 cccggttcgg atcgatcggg aggatcagcc gcaccgaccc gccgttcgag caggccggca
      301 tgtcgagcgt gctcctggat ggcgcgggga agctccgctc gatttcggtc atcccgccgc
      361 gagccgatac attggcggcg gcggcggagc ccgaatgggc accctggttc gggctcgccg
      421 ggctcgacac cgcccgcttc cacccggtcg agccggaacg gaacccgagc ttctacgtgg
      481 atggccgccg ggcctggcgc ggcacgctgg cctcctcccc cgccgactcg tacctggtgg
      541 agggcgccac ctaccgcgga cgactggccc gcttcgcggt gatcactccc tgggatccgg
      601 gcgatcggtt caacgacgcc gtgtcggccg gacagcggac ggcccagtcg gtcgtatcgt
      661 tcctcatcat catgaccgtc ctggtgggcg gcttcctgat cacccggcgg aaccttcggc
      721 tcgaaagggc cgatcggctg ggcgccggcc gcgtggcgct ggcggtgggg ggctcgctca
      781 tgctgacctg gctcttcacc gcgtcgcaca ccggcgacct ggcctcggag atcggcgtct
      841 tctggaacgg gatggccatc gcctgcctga acgggctcct ggcctggctc atctacatcg
      901 gggtggagcc gtacgtgagg cgggccaagc cgcatctcct gatcggatgg acgcgctttt
      961 tggccggcgg cttccgcgat ccgatcgtcg gacgcgacgt gctgatcggc gccgtggccg
     1021 gcgtcacgct ccacgtgctg caggggatcg gctggctggt gcccacctgg ctcggacagc
     1081 ccccggaaac gcccctctcg atcgatcccg acacgctgct cggcttcggg acgatgctcg
     1141 gcgccatcct ggccagcctg accaacgtcg tcacgacgcc gctggtcgtg ctcatcatct
     1201 acgtgatcgg tcggatggtc ttcaggaagg agtgggcggc gctggggctg gtggcgctgc
     1261 tcttcggcgg ggcggcgctg acgggccaga catcgaccgt tcggctggtc atcggcctca
     1321 tctacaccgc ggtgctcctg ctggtggtct tccgctgggg gttcctggcg gccggttcgg
     1381 cgatattggt cgaggggctc gtgtcgaact atccgttcgc catgaacccc tcgacctggt
     1441 acttcccgac cactcttgcc gtgctctcgc tggtggcgct gctcggctac tgggcctggc
     1501 gcgccgcccg cagccggctg atgctcgcgc ggcctacgac tcggtgacga cgatcttcga
     1561 cttcatcccc tcttcattga ggctgcccgc cgcgtaggac ttgaggtccg acacgcgcgc
     1621 cttcatctgg agctggtgca gcttgtgctt cttgaagtag cggctgtcgc tcagctcgac
     1681 cgacacgccc acccactggg tgtcggagag gcgacccgag aagttgtcgc cgccggccat
     1741 cagggcctcc atcatctcgt ccttgaactg ggcgtactgc ttctcctggc gggccagcat
     1801 cttggcgctg tcgtcatcgt aatcttcgtc gtcgtcatca tcgtcgtccc agccgaaact
     1861 ccagttggcc ttcttgccgt accaccagtg ccggttgagc agcgacacct cggcggtgaa
     1921 cagcacgccg aactccggga gatagtggcc ggtggtggct tcgt
//
LOCUS       DASVOD010000060         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_734766, whole genome shotgun sequence.
ACCESSION   DASVOD010000060 DASVOD010000000
VERSION     DASVOD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734766"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF720_01175"
     CDS             complement(<1..624)
                     /locus_tag="VF720_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877986.1"
                     /translation="MELKLPDLRIVDTKRLVLHEESDPSRSERIAERLAEEKVLGNPP
                     IVASVPGTSRLIVLDGANRVSAAFVLRLPALMVQVVDYEDPEVKLHRWHHLLLELDGE
                     ALLDSVRRIPCAEVEKTTATTAERALSGSRVACVVRPTGGSAWRVKPRPGVSPAAVMR
                     SVTDIYKRRTQIYRIADEQIDRLAEQTQANVTAVILFPGFRKKDIVRF"
     gene            complement(615..1628)
                     /locus_tag="VF720_01180"
     CDS             complement(615..1628)
                     /locus_tag="VF720_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX7877987.1"
                     /translation="MFPPVTEAAQAAWRRAVDEAALFDRSDRGRLVLKGGDALDLLHR
                     TTTADLTGLPIGGFATTVMQTETGRILDWLALWRRDNQLLLVTGPGRASDDLAWVDRM
                     VIMDDVMPELLDESTRLFELAGPRAPEVLAATGLAVATTGEAAIIDGTIGGISTTVAV
                     ARRGSRPNFWLMMPKEVAETVAGRLEKGGAIRVGADVDDAMRLERGEPLVGRELTEAA
                     NPLEARLDGSIGFQKGCYPGQEVIARMITYKSLKRRLSLMRLDDPLPSPPAGQSWPLL
                     EGAAAGRITSAAWSFAHGAPLAMGYLRNDLAVTGTAVTVAGPAGLVTGRVLDFPTEGT
                     RWS"
     gene            complement(1840..2442)
                     /locus_tag="VF720_01185"
     CDS             complement(1840..2442)
                     /locus_tag="VF720_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEX7877988.1"
                     /translation="MTRYAGIILAAGESRRMGQPKALLPWGGSTFVFTVTSRLLDAGL
                     DPVVVVTGAHADVIRASTIPDGAAWIENHEWRSGQAGSLRAGVRALPSNVTAAVVALV
                     DQPQIRTASVTRLLDAHAARGAPVVRPVFNGRGGHPYLIDSCVFPRILTAPADATTFA
                     ILKNFRESFDDVAFEDNSILIDFDTPGDLAREAPRFGEQF"
     gene            complement(2439..2960)
                     /locus_tag="VF720_01190"
     CDS             complement(2439..2960)
                     /locus_tag="VF720_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877989.1"
                     /translation="MRHRIVSSRLLVKAHPVHIYKFIENLENHKTLWPGASNWQGDKD
                     QARYRWQLGLGSFNVETRIVERHPGIRVCEEPVSGAPFPYRRYFKIQPDDQTAILNIS
                     IEAEMNGLQVALFHLPLKAQLEGILRNIQKTMEKPKPAVAASPMATPAVATPAPAAAP
                     VPTPSTPPNAASS"
     gene            3238..4173
                     /locus_tag="VF720_01195"
     CDS             3238..4173
                     /locus_tag="VF720_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7877990.1"
                     /translation="MSWSEAWPWVAFLAFVISLLVLDLGLLHRKEHVIRPREALLWSA
                     FWVSLAMAFCVLIYFWKGPAPAVEFLTGYLIEESLSVDNLFVFVLIFTYFRVPPEYQH
                     RVLFWGILGALVLRATFILAGAWLLSRFAWVEYIFGAFLLVTALRMLKGGEPEVHPEG
                     NGVVRLFRRFFPVTTDYHGTKFFIRENGVLAATPLVIVLILVETTDVVFALDSIPAIF
                     GVTRDPFIVFTSNIFAILGLRALYFLLASVLGLFAYLNIGLACVLAFIGLKMMLGHWV
                     HISTLASLLVVFGLLALSIVASLLFGRKKAHHGTP"
     gene            4188..5474
                     /locus_tag="VF720_01200"
     CDS             4188..5474
                     /locus_tag="VF720_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX7877991.1"
                     /translation="MIRPLRSPGKVPLPALLLSMAALGSGCAMPSPKDARAVIAARAF
                     QSTLPAYLRPVGHSPVPKGLTGMRAADCGACHVEIYAEWKESSHARAFRDDPQFMEEL
                     RKTTATSGRDASWICLNCHTPFEAQLPRLLAGLEKGDRGLPIYVDNPSFDPRMQEEGV
                     TCATCHVRDGVVLGPTGAGRAPHAVQRSDLLHSSDLCLQCHEVNARIDDVGLVCLFDT
                     GVTYAAGPDAAEGKSCQECHMPEVERPLTNLGTPPRTTRRHWFGGSLIPKKPEFEAAM
                     APIRAHYKSGALVAWTDLPEAFPAGKRATVTFSVTNAEAGHSLPTGDVERHIVITARL
                     RGSDGTLLAERAERFGTRYEWYPAARRLDDTRLGPRETRTFTLEFEAPSRGTVTLELE
                     GVHVRMTAENVAYHGLEGRTVPSRTFLDSREERPVR"
     gene            complement(5480..>5742)
                     /locus_tag="VF720_01205"
     CDS             complement(5480..>5742)
                     /locus_tag="VF720_01205"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7877992.1"
                     /translation="RIPHAISILVGDVLNTDLTMKTALELDALFTDKGISKDQEVITH
                     CYVGYRSSQGYFYFRLMGYNVTHYDGSWSDWNADPNTPKEHS"
BASE COUNT          931 a         1910 c         1885 g         1016 t
ORIGIN      
        1 gaaacggacg atgtccttct tccggaaccc gggaaagagg atgaccgccg tgacgttggc
       61 ctgcgtctgt tcggccagcc ggtcgatctg ctcgtccgcg atccggtaga tctgggtccg
      121 ccgcttgtag atgtcggtca cgctgcgcat gacggcggcg gggctcacgc cggggcgcgg
      181 cttcacgcgc caggccgacc caccggtggg ccgcaccacg caggccaccc ggcttcccga
      241 aagcgcccgt tcggcggtgg tggcggtcgt cttctcgacc tcggcgcagg gaatgcgacg
      301 gaccgaatcg agcagcgcct cgccatccag ttcgagcagc aggtgatgcc agcgatggag
      361 cttgacctcc gggtcttcgt aatcgaccac ctgcaccatg agggccggca ggcggagcac
      421 gaacgcggcg ctcacgcggt tggccccgtc cagcacgatc agccgggagg tgccgggcac
      481 gctggccacg atgggtggat tgccgagcac cttctcctcg gccaggcgtt cggcaatccg
      541 ctcgctgcgc gacggatccg attcctcgtg cagcaccagc cgtttcgtat cgacgatgcg
      601 caggtcaggc agcttcagct ccatcgcgtt ccttccgtcg gaaagtcgag cacgcgtccc
      661 gtcaccaatc cggccggccc ggccacggtg acggcggtgc cggtcacggc aagatcgttg
      721 cggagatatc ccatcgccag cggcgccccg tgggcaaacg accaggctgc cgaagtgatg
      781 cgaccggctg cggccccctc gaggagcggc cacgactggc cggccggcgg cgacgggagg
      841 gggtcatcga gacgcatgag ggaaagccgc cgcttcaatg acttgtaggt gatcatccgg
      901 gcgatgactt cctgcccggg gtaacatccc ttctggaagc cgatcgaccc gtcgagcctg
      961 gcctcgaggg gattggccgc ctccgtcagc tcgcggccga ccagcggttc cccacgctcg
     1021 agccgcatcg cgtcgtcgac atccgcgccg acacgaatgg caccgccctt ctcgagtcga
     1081 cccgccactg tttcggcgac ctccttcggc atcatcagcc agaaattcgg ccgactgccg
     1141 cgtcgcgcga cagccaccgt tgtcgaaatg ccgccgatcg tcccatcgat gatggcagcc
     1201 tcgccggtgg tggccaccgc gaggcccgtc gcagccagca cttccggcgc gcgcgggccg
     1261 gccagctcga acaaccgtgt cgactcatcc aggagctccg gcatcacgtc atccatgatg
     1321 accatgcggt ccacccacgc gaggtcatcg gaagcgcgac ccggcccggt cacgagcagg
     1381 agctggttat cacgtcgcca cagcgctaac caatcaagga tgcgtccggt ctctgtctgc
     1441 atcaccgtgg tggcaaagcc gccgatcggg agccccgtga ggtccgccgt cgtcgtgcga
     1501 tgcaggagat cgagcgcatc gccgcccttg agcacgaggc ggccgcgatc ggagcgatcg
     1561 aagagagccg cttcgtctac ggcccgccgc catgccgcct gcgcggcttc cgtcacgggt
     1621 gggaacatgg ccggggattg tgcggagcgc cggaaagacg ggtcaagagg gagtccgggg
     1681 agagccgtgg caccaaataa acgagccgcc gacacgtgtc ttcgtcccgt ggccgctcgg
     1741 gagtcgaagt gcgtcgacgg ctctgactca gggggtcggg agctttcgct cccgatttgt
     1801 cacatgcacg ggtggataca cggtggagga cgagaggttt cagaattgtt cgccgaagcg
     1861 cggcgcttct cgcgcgagat cgccgggcgt gtcgaagtcg atcaggatcg aattgtcttc
     1921 gaatgcaaca tcgtcaaacg attcacgaaa atttttcaat attgcaaagg tcgtggcatc
     1981 cgcgggcgcc gtgaggatcc ggggaaagac acaggaatca atgagatagg gatgcccgcc
     2041 gcgcccgttg aatacggggc gcacgaccgg cgcgccgcgc gccgcgtggg cgtcgagcag
     2101 gcgcgtcact gacgcagttc tgatctgcgg ttgatccacc agcgcaacga cggcggccgt
     2161 cacattcgaa gggagcgccc ggactcccgc ccgtagagat ccggcctgac cggaacgcca
     2221 ctcgtgattc tcgatccatg cggcaccatc gggaatcgtc gaagcccgaa tgacatcggc
     2281 atgcgcgccg gtgacaacga cgaccggatc aagcccggca tcgagaaggc gcgacgtcac
     2341 cgtgaacacg aaggtcgagc ccccccaggg gagcaacgcc ttcggttgac ccatccgccg
     2401 ggactcgcca gcggcgagga tgatcccggc ataacgggtc acgacgatgc ggcgttgggg
     2461 ggcgtggaag gtgtcgggac cggcgccgcc gcgggagccg gtgtggccac ggccggcgtg
     2521 gccatgggag acgcggcaac cgcgggcttg ggcttctcca tggtcttctg gatgttccgg
     2581 aggatcccct cgagctgcgc cttgagcggg agatggaaca gggccacctg caggccgttc
     2641 atctccgcct cgatcgagat gttgaggatg gcggtctggt cgtcgggctg gatcttgaag
     2701 tagcggcggt agggaaacgg cgcaccgctg acgggctctt cacagacccg gatgcccggg
     2761 tggcgttcga caatgcgggt ctcgacgttg aacgacccga gccccagctg ccagcggtag
     2821 cgggcctggt ccttgtctcc ctgccagttg gaggcgccgg gccacaacgt cttgtggttc
     2881 tcgaggttct cgatgaactt gtagatatgg acgggatggg ccttcacgag aaggcgggag
     2941 gacacgattc ggtggcgcat gtttacaggc tctcggtcag cggctcggcg gatcgggcgt
     3001 ggtgccggtc cgtcatggac cggagtcgtc acccggaagc ccactattat agggcatccg
     3061 gatggcatct cccggggaga aaatcggccg gatgccccgg attccttagg tgaacttttt
     3121 ccccatcccc ttttcccatc tggcgcaacg ccttaaagtc ggtgatccaa tgacgcggac
     3181 aggggggtgg cgtgctcttt tccagggagc cggtccccgc ccggcggggg ggctccggtg
     3241 agctggtccg aagcctggcc atgggtggcc ttcctcgctt tcgtcatttc cctcctggtt
     3301 ctcgacctcg gtctcctcca ccgcaaggaa cacgtcatcc gcccccgcga agcactcctc
     3361 tggagcgcct tctgggtatc gctcgcgatg gccttctgcg tcctgatcta tttctggaag
     3421 gggcctgcgc cggccgtcga gttcctgacc ggctacctga tcgaagaatc gctttccgtc
     3481 gacaatctgt tcgtttttgt cctcatcttc acctacttcc gggtgccgcc ggagtatcag
     3541 catcgcgtgc tcttctgggg gatcctgggc gcgctggtcc tccgggccac gttcatcctg
     3601 gccggcgcct ggctcctcag ccgcttcgcc tgggtcgagt acatcttcgg ggcgttcctg
     3661 ctggtcacgg cgctgcggat gctcaaggga ggggaacccg aggtccatcc cgaggggaac
     3721 ggcgtcgttc gcctcttccg tcgattcttt ccggtgacga ctgattatca cgggacaaag
     3781 ttcttcatcc gcgagaacgg cgtcctcgcc gccacgccgc tggtcatcgt gctcatcctg
     3841 gtcgagacga ccgacgtcgt cttcgcgctc gattccatcc cggccatctt cggtgtcacg
     3901 cgcgatccct tcatcgtctt cacatcgaac atcttcgcca tcctcgggct gcgcgcgctc
     3961 tacttcctcc tggccagcgt cctcgggctg ttcgcctacc tcaacatcgg tctggcttgc
     4021 gtcctcgcgt tcatcggcct caagatgatg ctcggccact gggtgcacat ctcgacgctg
     4081 gcgtcgctcc tcgtggtctt cggcctgctt gcgctgtcca tcgtggcttc gcttctgttc
     4141 ggccggaaaa aggcgcatca cggcaccccc tgagggacac cgcccgagtg atccgacccc
     4201 tccgttcgcc gggaaaagtc ccgctgccgg ccctgcttct ctcgatggcc gcattgggtt
     4261 cgggctgcgc gatgccctcc ccgaaggacg ccagggccgt gatcgccgcg cgcgcgttcc
     4321 aatcgacgct tccggcctac ctccggccgg tcggccattc gcccgtcccg aaggggctta
     4381 ccggaatgcg agcggccgac tgcggcgcct gccacgtcga gatctacgcc gagtggaagg
     4441 agtcgagcca cgcccgcgcc ttccgcgacg acccccagtt catggaggag ctcaggaaga
     4501 ccaccgccac ctccggccgc gacgcctcgt ggatatgcct gaactgccac acgcccttcg
     4561 aggcccagct cccgcggctc ctggcaggac tcgagaaagg ggatcgcggg ctccccattt
     4621 acgtggacaa cccgtccttc gatccgcgca tgcaggagga gggggtcact tgcgctacct
     4681 gccacgtgcg tgacggcgtg gtgctgggac cgaccggtgc ggggcgcgcg ccgcacgcgg
     4741 tgcaaaggag cgatctcctc cactcgtccg atctctgcct ccagtgccac gaggtaaacg
     4801 cccggatcga cgacgtaggc ctcgtctgcc tgttcgacac gggcgtgaca tacgctgccg
     4861 gccccgacgc cgccgaaggg aagagctgcc aggagtgcca catgccggag gtggagcgcc
     4921 ctcttaccaa cctcggcacg ccgccacgca ccaccaggcg ccactggttc ggcggctcac
     4981 tgatcccgaa gaagccggaa ttcgaggcgg cgatggcgcc gatccgtgcc cactacaaga
     5041 gcggcgcgct cgtggcctgg acggacctgc cggaagcgtt tcccgccggg aagcgcgcca
     5101 cggtcacctt ctcggtgacc aacgccgaag ccgggcactc ccttcccacg ggagacgtgg
     5161 agcgccacat cgtgatcacg gcccggctgc ggggatcgga cggcaccctc ctcgcggagc
     5221 gcgccgagcg cttcggcacc cggtacgagt ggtacccggc cgcacgccgg ctggacgaca
     5281 cgcggctggg cccgcgggag acgcgcacgt tcacgctgga gttcgaggcg ccatcacgcg
     5341 ggacggttac gctggagctg gaaggcgttc acgtgcggat gacggccgag aacgtggcct
     5401 accacggatt ggagggacgg acggtgccga gccggacgtt cctcgattcg cgcgaggaac
     5461 gtccggtccg atagtcgtac tacgagtgtt ccttcggggt gttcgggtcg gcgttccagt
     5521 cgctccacga tccgtcgtaa tgggttacgt tgtaccccat caggcggaag tagaaatatc
     5581 cctgcgagga acgatacccc acgtagcagt gggtgatgac ttcctggtcc ttcgagatcc
     5641 ccttgtccgt gaagagcgcg tcgagttcga gcgcggtctt catcgtgagg tccgtgttca
     5701 gcacgtcgcc caccaggatg ctgatggcgt gggggatgcg cg
//
LOCUS       DASVOD010000061         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_630176, whole genome shotgun sequence.
ACCESSION   DASVOD010000061 DASVOD010000000
VERSION     DASVOD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630176"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="glyS"
                     /locus_tag="VF720_01210"
     CDS             complement(<1..1002)
                     /gene="glyS"
                     /locus_tag="VF720_01210"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX7877993.1"
                     /translation="MTHDFLLEIGTEEIPAGYLRPAGEHLRATLTAGLVERRIDAASV
                     TVHEAPRRLIARITGLNDRQPDLSKEVMGPPSKAAFAPDGTLTKAGEGFARGQGVEPS
                     ALRRVDTPKGEYVAATVHVTGRPTADILLEWLPDVVRGIPFPKTMKWRGESLRFARPI
                     RWLVALLDAEVLPIQVETLRAGRATRGHRLVTPGPIEIESPGTMLTQLEGAGVIADFD
                     ARRSRVVNEVKSAATAASARIVADDDLVDEVTNLVELPTAVMGSFDPEYLELPREVVI
                     TAMKAHQRYFAVEGTDGALKPNFITVANGRWEDTSQVVAGNERVLRARLADARFYWDT
                     "
     gene            complement(999..1973)
                     /gene="glyQ"
                     /locus_tag="VF720_01215"
     CDS             complement(999..1973)
                     /gene="glyQ"
                     /locus_tag="VF720_01215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEX7877994.1"
                     /translation="MKTRETPTFQEMLLRLERYWSDLGCTILQPYPSEVGAGTFNPAT
                     FLRALGPEPWNVAYVEPSRRPKDGRYGENPNRMQNFYQYQVLLKPAPDNVLELYEKSL
                     EAIGIDLTRHDLRYVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQQVGSIDLDLTS
                     AEITYGIERIGTYLQGVNRVMDLRWTKDLNWSDLFLSGEVEWCHYNFEEANTELLFQL
                     FTANETEAQKLLAKGLVAPGYDHVIKCSHAFNLLEARGAISVTERTGYIGRVRKLARL
                     AALAYQEQRKAMNWPLLKAGPERDAWLAKVATEAKPDATDRRDPKANA"
     gene            complement(2029..>3916)
                     /locus_tag="VF720_01220"
     CDS             complement(2029..>3916)
                     /locus_tag="VF720_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877995.1"
                     /translation="FLGTVNQSEFGRAVAGPGDVNGDGRNDIAVGQPYVDNSLWIYSG
                     VDGSQLARYEPPNNVQIDFARSVSRVGSVSGDSRAEVLLGAPIGHGPGRRLAGQVWLV
                     PYGPNIDDSGSAEFMQLGTAVTTLGRRGTTGDFAAGGPGFTSLVRVYRPDGSTRFELT
                     GTPGTGFGSVLAGAGDVDGDGYPDLLVGAPGLDGGNQVGRAALHSGRNGSLVFEIFGT
                     AGDFTAQALSSAGDLDGDGGDEFAVGAPWADPPGAPDGGRVTIYSLPQPCLSVALKAD
                     PATCAGSQIPVLAILTNCGRLPLITSVLAPGEPMTPVVLEPGESIENPLSATPIACAG
                     DGPGHIDVTVQSRYDGCPTTSREVTRSLALVCETCGPVNCPRPLSFWERQCLNKGRGR
                     EVTAGELAAIAACVDVRSSMFDWDTEPGAFCRTIGGLPQESGRDAALREYSALLANSC
                     AASIIQGASPPIRLNLTTAIEFPEVPCATLACLATLVDARLLELADRDPALTEVMAAY
                     DEVRDLCRKVNAGEGIGTTCSASQLVDPPVSPIVRIDPTPLCPDDRVRLRITEADRGP
                     LTVDLHDVAGRRIRRLWSGDVDDATTIEFDFTATGARPAAGIYFLRVTSSSARRSVRL
                     VIVD"
BASE COUNT          620 a         1306 c         1342 g          648 t
ORIGIN      
        1 ggtgtcccag tagaagcggg catcggccag gcgggcgcgc agcacgcgct cgtttccggc
       61 caccacctgg ctcgtgtcct cccagcggcc gttggccacg gtgatgaaat tgggtttcag
      121 tgcgccgtcc gttccttcca cggcaaagta gcgctggtgc gccttcatgg cggtgatgac
      181 gacctctcgc gggagttcga gatattcggg gtcgaaactc cccatcaccg ccgtcggcag
      241 ttcgacgagg ttggtgactt catcgaccag gtcgtcatcg gccacgatgc gtgcggaggc
      301 cgctgtcgcg gccgatttga cctcgttgac gacgcggctc cgccgcgcat cgaagtcggc
      361 gatcaccccg gccccttcga gctgcgtcag catcgtcccc ggcgattcga tctcgatcgg
      421 gcccggcgtg accaggcgat ggccgcgcgt ggcgcgaccg gcccggagcg tctccacctg
      481 gatcggcagg acctcggcgt cgaggagcgc caccagccag cggatcggcc gcgcgaagcg
      541 gagcgactcg ccgcgccact tcatcgtctt cgggaacggg atgccgcgca cgacgtcggg
      601 gagccactcg aggaggatgt cggcggtcgg ccgccccgtg acatgcacgg tggccgccac
      661 gtactcacct ttcggcgtgt cgacgcggcg aagcgccgaa ggctcgacgc cctggccacg
      721 ggcaaacccc tcccccgctt tcgtcagagt gccgtccggc gcgaacgccg ccttcgacgg
      781 cgggcccatg acctcctttg agaggtcggg ctgccggtcg ttgaggccgg tgatccgcgc
      841 gatgagtcgg cgcggggctt cgtggacggt gacggacgct gcgtcgatcc gccgctcgac
      901 caggccggcg gtgagcgtgg cgcgcaagtg ctcgcccgcc gggcgcagat aaccggccgg
      961 gatttcctcg gtgccgattt cgaggaggaa gtcgtgggtc atgcgttggc cttcgggtcg
     1021 cgtctatcgg tggcgtccgg cttcgcctcg gtggccacct tcgccagcca ggcgtcgcgt
     1081 tccggaccgg ctttgagcaa cggccagttc atcgccttgc gctgctcctg gtaggccagc
     1141 gcggccaggc gcgccagctt gcgcacgcgg ccgatgtagc cggtccgctc ggtcacgctg
     1201 atcgcgccac gcgcctcgag caggttgaag gcgtggctgc acttgatgac gtggtcgtag
     1261 cccggcgcca ccagcccctt ggcaaggagt ttctgtgcct ccgtctcgtt ggccgtgaag
     1321 agctggaaga gaagctcggt gttggcttcc tcgaagttgt agtggcacca ctccacttcg
     1381 ccgctcagga agaggtcgga ccagttgagg tccttcgtcc aacgcaggtc catcacccgg
     1441 ttcacgccct ggagataggt gccgatccgc tcgatgccgt aggtgatctc ggcactggtc
     1501 aggtcgaggt cgatcgaacc gacctgctgg aagtaggtga actgcgtcac ttccatgccg
     1561 tccagccaca cttcccagcc gaggccccag gcgcccagcg ttgggctttc ccagtcgtcc
     1621 tccacgtagc ggaggtcgtg ccgggtgagg tcgatgccga tggcctcgag gctcttttcg
     1681 tacagctcca gcacgttgtc cggcgccggc ttcagcagca cctggtactg gtagaagttc
     1741 tgcatccggt tcggattctc gccgtaccgg ccatccttgg gccgccgcga gggttccacg
     1801 tacgccacgt tccacggctc cggacccagc gcgcggagga aggtggccgg gttgaacgtg
     1861 ccggcgccga cctcggaggg atagggctgc aagattgtgc agcccaggtc ggaccagtag
     1921 cgctcgaggc gcaggagcat ttcctggaag gtcggcgtct cgcgagtttt catggggcgg
     1981 ggagtatagg ggggcgggct tgcgggcggc aaccgcgggg ggttcgcgtc agtcgacgat
     2041 caccagccgg acactccggc gggcactcga tgaggtcacc cgcaggaaat agatcccggc
     2101 tgccggtcgt gcgccggtcg ccgtgaaatc aaactcgatt gtggtcgcgt catccacatc
     2161 cccgctccag agccgccgga ttcgacggcc ggccacgtca tgcaggtcga ccgtcagcgg
     2221 gccccggtct gcctcggtga tccgcagcct cacccgatcg tcggggcaga ggggcgtggg
     2281 atcgatacga acgatcggcg ataccggggg atccacgagc tggcttgcgg agcaggtcgt
     2341 gccgatcccc tctccggcat tcaccttccg gcacaggtcc cggacctcgt cgtaggccgc
     2401 catcacctcc gtgagcgctg gatcacgatc cgccagttcc agcaggcggg cgtcgaccag
     2461 cgtggccagg caggcgagcg tcgcgcaggg aacttcgggg aattcgatcg ccgtggtgag
     2521 attcaggcga atcggaggag acgccccttg gattatcgaa gccgcgcagc tgttggccag
     2581 gagggccgag tactcgcgga gggccgcatc gcgtcccgat tcctgcggca ggccaccgat
     2641 ggtgcggcag aatgcacccg gctcggtatc ccagtcgaac atggacgatc gtacgtcgac
     2701 gcatgccgcg atcgcggcga gttcgcccgc cgtcacttcc cgtccgcgac ctttgttgag
     2761 gcattggcgt tcccaaaagg agagcggacg cgggcagttg acggggccgc acgtctcaca
     2821 gaccagcgcg aggcttcggg tgacctcgcg cgacgtggtc gggcagccgt cgtaccggga
     2881 ttgcacggtg acatcaatgt gcccgggtcc gtcacccgca caggcgatcg gggtggccga
     2941 caagggattc tcgatcgact cgcctggctc caggaccacc ggcgtcattg gttcgcccgg
     3001 cgcgagaacg gacgtgatga gcggcagccg accacaattc gtgaggatgg ccagtaccgg
     3061 aatctgcgaa cccgcacagg tggccggatc ggccttcagg gccaccgaga ggcatggttg
     3121 cgggagcgag tagatggtca cgcgtccccc gtccggcgca cccggaggat cggcccacgg
     3181 ggctcccacc gcgaattcgt cgcccccgtc gccgtccagg tcccccgcgg acgagagggc
     3241 ctgggcggtg aaatctccgg ccgtgccaaa gatttcgaag acgagcgagc cgttccggcc
     3301 ggaatggagc gcggcgcgcc cgacctggtt gccaccatcg agcccaggcg cgccgacgag
     3361 gagatcggga tagccatcgc cgtcgacgtc ccccgccccg gccaggaccg acccgaagcc
     3421 ggttccgggc gtgcccgtga gttcgaatcg agtcgatccg tccggtcgat acacgcggac
     3481 gaggctcgtg aagccgggtc cacccgccgc gaaatctccc gtggtgccgc ggcgtccgag
     3541 cgtcgtgaca gcggttccca actgcatgaa ctcagcggaa ccgctgtcgt cgatattggg
     3601 cccatagggg acaagccaca cctgccccgc cagccggcgg ccagggccgt ggccgatcgg
     3661 cgccccgagg agcacctccg cgcgcgagtc gccggagacg cttccgacac gggagaccga
     3721 tcgggcaaag tcgatctgaa cattgttcgg cggctcgtaa cgggcgagct gagacccgtc
     3781 gacgcccgaa tagatccaga gcgagttgtc gacgtaaggc tgtcccacgg caatgtcgtt
     3841 gcgaccatcc ccgttcacat cccccggacc agccaccgca cggccgaact cggactggtt
     3901 caccgtgccg aggaat
//
LOCUS       DASVOD010000062         1895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_212898, whole genome shotgun sequence.
ACCESSION   DASVOD010000062 DASVOD010000000
VERSION     DASVOD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212898"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF720_01225"
     CDS             <1..403
                     /locus_tag="VF720_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7877996.1"
                     /translation="VPGGANAENAKPDSLGWMGPYSIQGFGRSPDRELAVRIAMKLAD
                     QSNYVPNDSPDCPFYPRHGLRYISTTRDTVDVLLSFNCSLIMVVTSDGKEGWGGDFSP
                     VGEDVKGMVTPLLPVVIQPVDKSLSKSGLRR"
     gene            complement(400..1839)
                     /locus_tag="VF720_01230"
     CDS             complement(400..1839)
                     /locus_tag="VF720_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010872170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX7877997.1"
                     /translation="MTPSLAAAAYLLAAVLFIMSLGGLSTQANARRGNLLGIVGMILA
                     VGITAVAFRAGSLPLFLGVVAVASVIGALLASRVSMTAMPELVAILHSFVGLAAVLVG
                     IATHLAPEGHHSGVEMMIHRVEVWLGVFVGAITFTGSIVAFGKLRGMIRSKPLVLPGR
                     HALNLIVFLACLGLAVPFLGSPDADGGLVPLLIMTALAGFLGVHLVMAIGGADMPVVV
                     SVLNSYSGWAAAAAGFMLGNDLLIVTGALVGSSGAILSIIMCRAMNRSIVSVILGGFG
                     AEEGAAPAAAGSAPAGEVVSIDAAATAELLQQARSVIIVPGYGMAVAHAQHPLWEVAQ
                     RLRERRTEVRFAVHPVAGRLPGHMNVLLAEANVPYDIVLEMDEINHDFPHTDAVLVIG
                     ANDIVNPGAMDDPASPIYGMPVLEVWKARTVVVMKRSMASGYAGVDNPLFYKKNTRML
                     FGDAKKTVEGILDALRATKGEMEMSGAGR"
BASE COUNT          328 a          616 c          681 g          270 t
ORIGIN      
        1 cgttcccggc ggcgccaacg cggagaatgc gaagcccgac agcctcgggt ggatgggccc
       61 gtactcgatc cagggcttcg gccggtcgcc ggaccgtgag ctggcggtcc gaatcgccat
      121 gaagctggcc gaccagtcga actacgtccc gaacgactcg cccgactgtc cgttctaccc
      181 gcgccacggg ctgcgctaca tcagcacgac gagggacacg gtggacgtgc tgctcagctt
      241 caactgctcg ctgatcatgg tcgtgacgag cgacgggaag gaaggttggg gcggcgattt
      301 ctcgccggtc ggcgaggacg tgaagggaat ggtgacgccg ctgttgccgg tggtcattca
      361 gccggtggac aagtcgctgt cgaaaagcgg actgaggcgc tagcgccccg cgccgctcat
      421 ctccatctcg cccttcgtcg cgcgaagcgc atccaatatc ccttccacgg tcttcttcgc
      481 gtcgccgaag agcatccgcg tgttcttctt gtagaagagc ggattgtcga cgcccgcgta
      541 gccggaggcc atggagcgct tcatcacgac gacggtcctg gccttccaga cctcgaggac
      601 gggcatgccg tagatggggc ttgcggggtc gtccatggcg ccggggttca cgatgtcgtt
      661 ggcaccgatg acgaggacgg cgtcggtgtg cgggaagtcg tgattgatct cgtccatctc
      721 gaggacgatg tcgtacggca cgttggcctc ggccagcagc acgttcatgt ggcccgggag
      781 gcgccccgcc acggggtgca cggcgaaacg cacttcggtg cggcgttcgc gcaggcgctg
      841 ggccacttcc cacaacgggt gctgggcgtg ggccaccgcc atgccgtagc cgggcacgat
      901 gatgacgctg cgggcctgct gcagcagttc ggcggtggcg gcggcgtcga tcgacaccac
      961 ctcgccggcg ggcgcggagc cggcggcggc gggtgcggct ccctcctcgg cgccgaaacc
     1021 gccgaggatg acgctcacga tcgagcggtt catcgcccgg cacatgatga tggagaggat
     1081 ggcgccggac gagccgacga gcgcgccggt cacgatgagc aggtcgttac cgagcatgaa
     1141 accggcggcg gcggccgccc agcccgagta gctgttcagg accgacacca ccaccggcat
     1201 gtcggcgccg ccgatcgcca tcacgaggtg cacaccgagg aagccggcca gcgcggtcat
     1261 gatgagcagc ggcacgaggc cgccgtcggc gtcgggcgag cccaggaagg gcaccgcaag
     1321 gccgagacag gccaggaaca cgatcaggtt gagcgcgtgg cggccgggca gcaccagcgg
     1381 cttgctgcgg atcatgccgc gcagcttgcc gaaggccacg atcgagccgg tgaacgtgat
     1441 ggcgccgacg aagacaccga gccacacttc cacgcggtgg atcatcatct cgacaccgct
     1501 gtgatgcccc tccggcgcca ggtgcgtggc gatgccgacg agcacggccg ccagccccac
     1561 gaagctgtgc aggatggcca cgagctcggg catggcggtc atcgacacgc gcgaggccag
     1621 cagcgccccg atgacggaag ccaccgccac cacgccgagg aagagcggaa ggctccccgc
     1681 gcggaaggcc accgcggtga tccccacggc caggatcatg ccgacgatgc cgagcaggtt
     1741 gccgcgccgc gcgttggcct gcgtggagag gccgccgagg ctcatgatga agaggacggc
     1801 ggccaggagg tacgcggcgg cggcgaggct gggagtcatc gtcatggtta tttccggaac
     1861 atcgccagca tgcgctgggt gacgaggaag ccgcc
//
LOCUS       DASVOD010000063         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_734852, whole genome shotgun sequence.
ACCESSION   DASVOD010000063 DASVOD010000000
VERSION     DASVOD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734852"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..728)
                     /locus_tag="VF720_01235"
     CDS             complement(168..728)
                     /locus_tag="VF720_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7877998.1"
                     /translation="MDTQTITATLAELTRGDATAIEKLLPVVYDEMRRLAASYLRKER
                     VDHTLQPTALAHEAFLKLVVQKDIAWQNRAHFMGVAAQAMRRILVDHARRVKAARRGG
                     GRTMVLLEEADVADPHQVEFDDLDRALEDLTRLSERQARVVELRYFGGLTIDEVGEVL
                     GVSPMTVKRDWTMARAWLFRELSGGA"
     gene            complement(793..1890)
                     /locus_tag="VF720_01240"
     CDS             complement(793..1890)
                     /locus_tag="VF720_01240"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX7877999.1"
                     /translation="MGCAHVRHYRSALLPAVPVRGGRDALRGHREDRSLKVLIANEAD
                     VHAMLPMDGCVALMREALISQFRVDAVLPLRSKMFLPDKSGLLGLMPGYLGTPRSFGL
                     KVVSVMPGNHGTPYDSHQGVVMLFGLEHGEPLAMMDASAITAIRTAAASGAATDALAN
                     PDAGDLAIIGSGTQARTHLVAMKTVRRLRRIRIWSRSKANAERFAHDNHHGEQPRIEV
                     MDTAEAAVRGADLVCTTTSAPEPVVKGAWLSPGAHINAAGACFPHTRELDTEAVVRAR
                     LFTDCRESCENEAGGYRLPLAEGAITSGHLLGEIGGVFIGELPGRTSPEEITLYESLG
                     IALEDLASAHAIHRRAVETGTGTWVEWGGPA"
     gene            complement(1785..>2411)
                     /locus_tag="VF720_01245"
     CDS             complement(1785..>2411)
                     /locus_tag="VF720_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878000.1"
                     /translation="SAGFVQSIAGGCQDIAGPPATFVSLTKGDAVGAYATRLWTSAPP
                     WDANGTFYDIVAGGALHDDAGDFGMTVATAFDDIAVTDDIDRQFRPGGTPPHADRGPD
                     QLEIASTSAVAEPPEPEDALSASARWTAGGYLAIDYHAEVSGRLELIIVDVTGRKRQS
                     DGREVSANARGRLSWDARTSGITGPLYYRLFLYGVDGTLSVATGRIDR"
BASE COUNT          389 a          855 c          797 g          370 t
ORIGIN      
        1 cgccgcggca aggcctggta cgcctcgagc ctctccatct accgcgaact ggaaaagcgg
       61 aaactgttgc tggtggagga tgcgacggcc ccggccgatg tggcgcggct ggccggtgaa
      121 ctgcagggcg cgaatcactg acggcaactg gtcccgggac cagtcgatca cgccccgccg
      181 ctcaactccc ggaacagcca cgcccgcgcc atcgtccagt cacgcttgac cgtcatcggc
      241 gacacgccaa gcacctcgcc cacttcgtcg atcgtgagcc cgccgaaata gcgcagctcc
      301 acgacgcgcg cctgtcgctc ggagagccgg gtcagatcct caagcgcgcg gtcgaggtcg
      361 tcgaactcca cctggtgggg atcggccaca tcggcttcct cgagcagcac catcgtgcgc
      421 ccgccgccgc gccgcgccgc cttcaccctg cgcgcatgat ccaccaggat ccgccgcatc
      481 gcctgtgccg ccacgcccat gaagtgcgcg cggttctgcc aggcgatgtc cttctggacg
      541 accagcttga gaaaggcctc gtgcgccagc gcggtgggct gaagcgtatg gtccacccgt
      601 tccttgcgga ggtacgaggc ggccagccgg cgcatctcgt cgtacacgac cggcaggagc
      661 ttctcgatgg cggtagcgtc accgcgggtg agctcggcga gcgtggcggt gatcgtctgg
      721 gtatccatgc agggcggccg gctccgggtg aactcgagga gacgtaacga ttataccgga
      781 cgcgaccgga tctcaggccg gcccgcccca ttccacccac gtccccgtac ctgtctccac
      841 cgcccgccgg tggatggcat gcgcggacgc caggtcttcc agggcgatgc cgagcgactc
      901 gtaaagcgtg atctcctccg gcgacgtgcg ccccggcagc tcaccgatga acacgccccc
      961 gatttcaccg aggagatgcc cgctcgtgat ggccccttcg gccagcggca accggtagcc
     1021 gcccgcctcg ttctcgcacg actcgcggca atcggtgaac agacgcgcgc gcacgacggc
     1081 ttcggtatcg agttcgcggg tgtgcggaaa gcaggccccc gccgcgttga tgtgcgcgcc
     1141 cggtgacaac cacgcccctt tcaccacggg ctccggcgcc gacgtcgtcg tgcagacgag
     1201 atccgcgccg cgcacggccg cctcggctgt atccatcact tcgatgcgcg gctgttcgcc
     1261 atgatgattg tcatgcgcga agcgctccgc attggctttc gaacgactcc agatgcggat
     1321 gcgccgcagg cggcgcacgg ttttcatggc caccagatgc gtgcgcgcct gcgtccccga
     1381 gccgatgatg gccaggtcac ccgcgtcggg attggccaac gcatccgttg ccgcgcccga
     1441 ggccgccgcg gtccggatgg cggtgatggc gctcgcatcc atcatcgcca gcggttcgcc
     1501 gtgctcgagc ccgaacaaca tcacgacacc ctggtgggag tcgtacggcg tgccgtggtt
     1561 gccgggcatg acgctcacga ccttgaggcc gaaactgcgc ggcgtgccca ggtagccggg
     1621 catcagcccg agcaacccgg acttgtcggg aaggaacatc tttgaacgga gcggcaggac
     1681 ggcatcgacc cggaactgcg aaatgagcgc ctcgcgcatg agcgccacgc acccatccat
     1741 cggcaacatg gcgtggacat cggcttcgtt ggcaatcaaa actttcagcg atcgatcctc
     1801 ccggtggcca cggagagcgt cccgtccacc ccgtacagga acagccggta atagagcgga
     1861 ccggtaatgc cggacgtgcg cgcatcccac gagaggcgcc cgcgcgcgtt ggccgacacc
     1921 tcgcggccat ccgactggcg cttccggccg gtcacgtcca cgatgatcag ctcgagccgc
     1981 ccggacacct cggcatggta atcgatggcg agataaccgc cggccgtcca gcgggccgag
     2041 gcgctgagcg cgtcctccgg ctcgggcggt tcggccacgg ccgacgtgga tgcgatttcg
     2101 agctggtccg gtcctcgatc ggcgtggggc ggcgtgccgc ccgggcggaa ctgccggtcg
     2161 atgtcgtccg tcaccgcgat gtcgtcgaag gcggtggcca ccgtcatgcc gaagtcgccc
     2221 gcgtcatcgt gcagcgcccc accggccacg atgtcgtaga acgtgccgtt ggcgtcccag
     2281 ggcggggcgg aggtccagag gcgtgtggca taggcgccga cggcatcccc cttggtgagc
     2341 gacacgaacg tggccggcgg cccggcgatg tcctggcagc cgccggcgat gctctgcacg
     2401 aagcccgccg a
//
LOCUS       DASVOD010000064         5041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_630304, whole genome shotgun sequence.
ACCESSION   DASVOD010000064 DASVOD010000000
VERSION     DASVOD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630304"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /gene="ftcD"
                     /locus_tag="VF720_01250"
     CDS             complement(<1..831)
                     /gene="ftcD"
                     /locus_tag="VF720_01250"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX7878001.1"
                     /translation="MKPIVECVPNFSEGRDPAVLDAIVSAMTAVPGVTLLGREMDASH
                     NRSVVTLVGDGEAVLEAAFRGAKEALARIDLTTHQGEHPRMGAVDVIPFVPVRGVTMA
                     DCVELAKRLGKRLGEELNFPVFLYEEAATRPERKNLADVRRGQFEGLRETIPVDPARI
                     PDFGPRAVHPTGGAVAVGARPFLVACNVYLGTPDVQVAKKIAAAVRGANGGLQFVKGL
                     GFFIEERGLAQVSMNLVNTTKTPIHRVVEMIRSEAARYGVAVLDTEIVGLVPEDALLD
                     A"
     gene            complement(947..1813)
                     /locus_tag="VF720_01255"
     CDS             complement(947..1813)
                     /locus_tag="VF720_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7878002.1"
                     /translation="MTTSRAHAYYEELAAAHVLGVLEEPERSELVAHITEGCSICRVV
                     YPQLVDVADLLLLGADAAAPPPGLKERIMAAATGTGTGSAGSTDVDRPVGPSPPLRLP
                     TRASSREPLYRGLALAASLALVLLTWQSLTWKKERDAARQSLLAESGQVSQLREEIRG
                     LEAGRAEQARLLELIERPGSGLVTLASLDPAPGALGKVLYDSAAGKGYLWVKHLPVDA
                     EGKDYQLWAIQGGAPVSAGVFSVTADGSALIPLADVRPDTRVAAFAITLEPAGGLPAP
                     SGPMVLLGNTGS"
     gene            complement(1810..2415)
                     /locus_tag="VF720_01260"
     CDS             complement(1810..2415)
                     /locus_tag="VF720_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7878003.1"
                     /translation="MLKFRSEGTQEGEPEARLVERLRAREGQALSELYDQYGPIVFAI
                     ARRIVKSPGDAEEVTQEVFLYAWEKAHLFDPARGTLFTWLGTLARSRGIDRLRNQKSQ
                     ERRRDALSRAVSAEPRPRDSDPRRELDLIEAKDRVRGALKQLPPEQREPIEIAYFEGL
                     TQSEIAARLEKPLGTIKTRMRQGMLRLRESMLPLLAEESQP"
     gene            2670..4427
                     /locus_tag="VF720_01265"
     CDS             2670..4427
                     /locus_tag="VF720_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX7878004.1"
                     /translation="MTRRLPVTLVTTFLLGIAPAILHASSHSEAPGTAKDRLVDDTDL
                     YAFVAADAPDAVTFVGNWVPLLEPNGGPNFYGFDDAAEYYINIDNVGDCLDHIRFEFE
                     FTTHRRNGATFLYNTGAVTSLNDPDLNIYQTAAITRIDNGSETVVATDVPVAPNYVGP
                     VSMPDYEALAASAVTTLGDGTKVFIGPRDDPFFVDLGAVFDLLTIRKVPGNKGKGVDG
                     VSGYNVLTIAIQVPMTRLTKDGLPPNAGNSVIGIYDSAERPATRTLNADGTISNSGPS
                     IQVSRLGSPLVNEVVIPLQDKNKFNATKPTGDGAFLPYVLNPELGSLLNLLYGITVPP
                     APRNDLVAAFLTGVPTLNNIPGSTPCEMLRLNMAIPPAAKPKRLAVLEGDAAGFPNGR
                     RLADDVVDIAERVVAGVLVPGFNVAPNNQLGDGIDVNDKPYLPYFPYVAPPHNPLDRR
                     GHSIQNGASPASLSTTSEGSIREWTDLAPADPATSPAMDGAAVLARTPSLSLASGPGA
                     GADLRYTVTEAGRVSVRIYDVQGRLVRNLLDQDAAPGEFRAPWDGRDDSGTNAGTGLY
                     FARYMAGGQPVNTTKIVLQ"
     gene            4600..>5041
                     /locus_tag="VF720_01270"
     CDS             4600..>5041
                     /locus_tag="VF720_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878005.1"
                     /translation="MSCLLAPSPAVAHEYWLSATRYRAARGEPVAISAWVGTGFRGDP
                     KLYAASRTASLEMLTDRRIDLTPLAAEADSHFCRVQAPDDSGFVVAFLSNYSVVDLEG
                     PRFEDYLKTEGLDEIVAIRHQRGEQQKRGTERYRRCAKTWITGPA"
BASE COUNT          825 a         1844 c         1578 g          794 t
ORIGIN      
        1 cgcatccagc agcgcatctt cgggaacgag tccgacgatc tccgtgtcga gaaccgccac
       61 gccgtagcgg gcggcctccg agcggatcat ctccaccacg cggtggatcg gcgtcttcgt
      121 ggtgttgacc aggttcatcg atacctgcgc gaggccgcgc tcctcgatga agaagccgag
      181 acccttgacg aattgaagtc cgccgttggc tccacggact gccgccgcga ttttcttggc
      241 cacctgcaca tccggcgtgc cgaggtagac gttgcaggcc accaggaatg gccgcgcccc
      301 gacggccacg gctccgccgg tcggatgcac ggcacgcggt ccgaagtccg gaatccgtgc
      361 cggatcgacc gggatggtct cgcgcaaccc ttcgaactgt ccgcggcgca cgtccgccag
      421 gttcttgcgc tccggccgcg tcgcggcctc ctcgtaaagg aagaccggga aattcaattc
      481 ctcgccgagc cgcttcccga gccgcttcgc cagctccacg caatcggcca tcgtgacgcc
      541 ccggaccggc acgaacggga tgacatccac cgcgcccatc cgcggatgtt ctccctggtg
      601 agtggtgagg tcgatccgcg ccagcgcttc cttcgcgccg cgaaacgccg cctcgagcac
      661 ggcctcgccg tcgcccacca gcgtcaccac cgaacggttg tggctggcat ccatttcgcg
      721 cccgaggagt gtgacgcccg gaacggcggt catggccgag acgatggcgt cgaggacggc
      781 gggatcacgc ccctcgctga aattgggaac gcattcgacg atcggcttca tggcagggcg
      841 cctcccgggc tcgacaatcc gacactcgct gaagaggccg gggaggatag caccggaggc
      901 gggccatggc agccggggca tggtaaccgg tgcctggcga cgtgcctcag ctgccggtgt
      961 tgcccaggag caccatcggt ccggacggcg ccggaaggcc ccccgccggt tcgagcgtga
     1021 tggcgaaggc cgcgaccctg gtgtcggggc ggacatcggc cagcgggatc agcgcggagc
     1081 cgtcggcggt gaccgagaag acgccggccg aaaccggggc gccaccctgg atcgcccaga
     1141 gctggtagtc cttcccctcg gcatccaccg ggagatgctt cacccagagg tatcccttgc
     1201 cggccgccga atcgtagagc accttgccca aggcgccggg cgccgggtcg agcgaggcga
     1261 gcgtgacgag ccccgacccc ggccgctcga tcagctcgag caggcgggcc tgttcggcgc
     1321 gaccggcctc gagcccccgg atctcctcgc gaagctggct cacctggccc gactcggcga
     1381 ggaggctctg ccgcgcggca tcccgctcct tcttccaggt gagcgactgc cacgtgagga
     1441 gcaccagtgc cagcgaggcg gccagcgcca ggccgcggta gagcggctcg cgagaggagg
     1501 cgcgcgtcgg gagtcgaagc ggcggcgacg gcccgaccgg tcgatcgacg tctgtcgaac
     1561 ccgccgatcc cgtccccgtc ccggtggccg cggccatgat ccgctccttg agcccggggg
     1621 gcggggccgc ggcatccgct ccgagaagga gaaggtcggc cacgtcgacc aactgcggat
     1681 aaacgacgcg gcagatcgaa caaccctcgg tgatgtgggc cacgagctcg ctccgttccg
     1741 gctcttccag gacacccagg acatgggcgg ccgccagttc ctcgtagtac gcgtgcgcgc
     1801 gcgaggtggt catggctgcg actcctcggc caggagggga agcattgatt cacgcagccg
     1861 cagcatcccc tggcgcatgc gcgtcttgat ggtgccgagc ggtttctcga gccgggcggc
     1921 aatttcggac tgggtgagcc cctcgaagta ggcgatctcg atcggctcgc gctgttcggg
     1981 cggcagttgt ttcagcgcgc cacgcacgcg gtccttcgcc tcgatcaggt cgagctcgcg
     2041 acgtggatcg gaatcgcgcg ggcggggctc ggcggaaacc gcgcgtgaaa gcgcatcacg
     2101 ccgccgctcc tggctcttct ggtttcgaag ccggtcgatg ccgcggctcc gggccagcgt
     2161 gccgagccag gtgaagagcg tgccgcgcgc cggatcgaag aggtgggcct tttcccacgc
     2221 gtacaggaag acttcctgcg tgacctcttc cgcatcaccc ggcgatttca cgatgcgccg
     2281 ggcgatggcg aagacgatcg ggccgtactg gtcatagagc tccgacagtg cctggccttc
     2341 ccgggcgcgc aggcgctcga ccaaccgcgc ttccggctct ccttcctgcg tgccttcgga
     2401 tcgaaacttg agcacccgaa ctcctcccgg tacggaggat atacgaaaga tgcggtgatg
     2461 cggattgggg catggagcga tcccccatcg tgatggtgcg actccccatt cggcgtcgcc
     2521 cggcaatcca cggcgccacg ggccccgtaa tcagccggac gcgcgacccg tttcggccga
     2581 cccgccgccg tcccaccagc ggcccgccct ccggactttc cggaaaccgc gcggtcaccg
     2641 ccatcacctc tgtcagggga gtgcttcaca tgacacgccg tcttcccgtc acgctcgtga
     2701 cgacctttct cctcgggatc gctccggcga tcctgcatgc ttccagccac agcgaagcgc
     2761 ccggaacggc caaggaccgc ctggtcgacg acaccgatct ctacgccttc gtggccgccg
     2821 acgcgcccga cgccgtcacg ttcgtcggca actgggtacc gctgctcgaa ccaaacggtg
     2881 gccccaactt ctatggcttc gacgacgccg ccgagtacta catcaacatc gacaacgtcg
     2941 gcgattgcct cgatcacatc cgcttcgagt tcgagttcac gacgcaccga aggaatggcg
     3001 ccacgttcct gtacaacacc ggtgccgtca cctcgctgaa cgatcccgat ctcaacatct
     3061 accagaccgc cgcgatcacc cgcatcgaca acggatcgga gaccgtcgtg gccaccgatg
     3121 tgccggtggc gcccaactac gtgggccccg tgtcgatgcc cgattacgaa gcgctggccg
     3181 cttcggccgt caccacgctc ggcgacggca cgaaggtgtt catcggcccg cgcgacgatc
     3241 ccttcttcgt ggatctcggc gccgtgttcg acctgctcac gatccgcaag gtgcccggca
     3301 acaaggggaa gggtgtggac ggcgtgagcg gctacaacgt gctgaccatc gccatccagg
     3361 ttccgatgac gcgactcacg aaggacggcc tgccgcccaa cgccggcaac tcggtgatcg
     3421 ggatctacga ttcggccgag cgtcccgcca cgcgtacgct caatgccgac ggtacgatct
     3481 cgaactccgg cccgtcgatc caggtgtcgc gccttggttc gcccctggtg aacgaagtgg
     3541 tcattcctct gcaggacaag aacaagttca acgccacgaa gcccaccggc gacggcgcgt
     3601 tcctgccgta tgtgctgaac cccgagctgg gcagcctcct gaacctgctg tacggcatca
     3661 ccgttccgcc cgccccgcgc aacgacctcg tggccgcgtt cctgaccggc gtccccacgc
     3721 tgaacaacat ccccggctcg accccctgcg agatgctccg cctcaacatg gccatcccgc
     3781 cggccgccaa gcccaagcgc ctggccgtcc tcgagggaga tgccgccggc ttcccgaacg
     3841 gccgccgact ggccgacgac gtggtggaca tcgccgaacg ggtcgtggcc ggcgtcctgg
     3901 tgcccggctt caacgtggca ccgaacaacc agctcggcga cggcatcgac gtgaacgaca
     3961 agccgtacct cccctacttc ccgtatgtgg cgccgcccca caacccgctc gaccgccgcg
     4021 ggcactcgat ccagaacggc gcgtcgccgg ccagcctgtc gacgacgtcg gaaggatcga
     4081 tccgtgagtg gaccgacctc gcgccggccg atcccgcgac aagccccgcg atggacggtg
     4141 ccgccgtcct ggcgcgcacc ccgtccctct ccctggcctc cggtcccggc gccggcgccg
     4201 acctccgtta caccgtcacc gaagccggcc gcgtgtcggt ccggatctac gacgtgcagg
     4261 gtcgcctcgt ccgcaatctg ctcgaccagg acgccgcccc gggcgagttc cgtgccccgt
     4321 gggacggccg tgacgacagc ggcacgaacg ccggcaccgg cctctacttc gcgcgctaca
     4381 tggccggcgg ccagcccgtg aacaccacca agattgtctt gcagtagact gtttcccgat
     4441 gagagcgaca caccgtcatg tgaaccctga atgcgaatga cacagaacca gaatcggaaa
     4501 gagtactcca tcggacgggc ggccctcggg ccgctcgtcc tctctattcc tcgccctgtt
     4561 tctgcgacat cgtttcaccg gtggttagca accattctga tgtcctgtct cctcgcccca
     4621 tcccccgccg tggcccacga gtactggctc tccgccaccc gctaccgcgc cgcccgtggc
     4681 gaacccgtcg ccatctccgc ctgggtcggc accggcttcc gcggcgaccc caagctctac
     4741 gccgcttcgc gcaccgcctc gctcgagatg ctcaccgacc ggcgcatcga tctcaccccg
     4801 ctggcggccg aggccgattc ccacttctgc cgcgtgcagg cgcccgacga ctcggggttc
     4861 gtcgtggcct tcctctccaa ttacagcgtg gtcgatctcg aagggcctcg tttcgaggac
     4921 tatctcaaga cggaaggact cgacgagatc gtcgccatcc gccaccagcg cggcgagcag
     4981 cagaagcggg gcaccgaacg ctatcgccgc tgcgcgaaga cctggatcac cggacccgcc
     5041 t
//
LOCUS       DASVOD010000065         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_839154, whole genome shotgun sequence.
ACCESSION   DASVOD010000065 DASVOD010000000
VERSION     DASVOD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839154"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /gene="lpxC"
                     /locus_tag="VF720_01275"
     CDS             complement(<1..1091)
                     /gene="lpxC"
                     /locus_tag="VF720_01275"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEX7878006.1"
                     /translation="MSQQRTIGGPATLSGVGLHSGQPTTLNFRPAAPNSGVVFERVDL
                     PGRPKIPVRPESATYDPGAGRRTILRSNGAEVHTVEHVLAAVAGLGVDNLVIEIDGQE
                     AAEPTDGSALPIARALLGAGIVEQSAEKRWARIPGPVSLNRGGVELIGIPHEGLRISF
                     TIEYDSPAVGTQVASFDISPEVFLNEIAPARTFVMHRDVELLRSQGLIRGGSLDNAIV
                     ITDSGILNEKPLRFKDELVRHKVLDLLGDLFLLGRPLKGHVIARRSGHQTNVEFVKQI
                     HAAENRSAGLGDLPPVPVEGKTPPWDIDTIMKLMPHRYPLLLIDRIVHMTDDKVIGLK
                     SVTINEPFFAGHFPGHPIMPAVLIIEAMA"
     gene            complement(1088..2152)
                     /gene="lpxD"
                     /locus_tag="VF720_01280"
     CDS             complement(1088..2152)
                     /gene="lpxD"
                     /locus_tag="VF720_01280"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEX7878007.1"
                     /translation="MPSFLTSAIAKAIGGQLDGTVDPLITGVAGIREAGPGEIAFLAH
                     PRYASYVSSTRAHALIVGHDHERGQGEGPVLIRVTDPYVGFLQTLHLFGGQRPRPAAG
                     IHPTAVVDAGASMGAGASIGPNVVVESGATLGERVTLMAGVYVGADCRLGDDVTVYPN
                     VVLRDAITIGNRVIIHPGAVIGADGFGYVKAGGVQLKVPQLGTVRVDDDVEIGANATI
                     DRATTGVTHIGAGVKIDNLVQIAHNVTIGPHSIVCAQVGVSGSTHIGGDVTLGGQAGV
                     GGHLKIGDRSMIGGQAGVTKSVPDDARVSGYPASDHEQALRVQAHTRRLPELVREIRA
                     LTARLGELERELEKERERVL"
     gene            complement(2200..2754)
                     /locus_tag="VF720_01285"
     CDS             complement(2200..2754)
                     /locus_tag="VF720_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX7878008.1"
                     /translation="MRSSPLFRALILAAALINCATIALAADYKIGYIDSEKVFREYTG
                     TKSAESEFNQDLEGWARQFENKKAELDKATREFEAQKLMLSDARRKERETELQSRISE
                     LEQLQREIWGPTGKVAQRNQQLTKDIVLKIRDVTLRIATAEGYTFILDAADGNLVYGD
                     PALDLTDRVIGELNQTNPGASTPR"
     gene            complement(2805..>3060)
                     /locus_tag="VF720_01290"
     CDS             complement(2805..>3060)
                     /locus_tag="VF720_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878009.1"
                     /translation="IVEPLHGVIFAEAGGTWNDLSDFRWDSLHKAAGIGARMEIPLLG
                     LVGFDYAYGFDRLNRNIIPGRGGHYDRGGWQGHLLFGRFF"
BASE COUNT          453 a         1027 c         1047 g          533 t
ORIGIN      
        1 tgggccatgg cctcgatgat caggaccgcc ggcatgatcg ggtgtcccgg aaagtgcccg
       61 gcgaagaacg gttcgttgat cgtgacgctc ttcagcccga tcaccttgtc gtcggtcatg
      121 tgcacgatcc ggtcgatcag gaggagcggg tagcggtggg gcatcaactt catgatggtg
      181 tcgatgtccc acggcggcgt tttcccttcc accggaaccg gcggcaggtc gccgagtccg
      241 gccgagcggt tttccgcggc gtggatctgc ttcacgaact ccacgttggt ctggtgcccg
      301 gagcgccggg cgatcacgtg ccccttgagc ggccggccga gcaggaagag gtcgcccagg
      361 aggtcgagca ccttgtggcg caccagctca tccttgaagc ggagcggctt ctcgttgagg
      421 atgccgctgt cggtgatgac gatggcgttg tcgaggctcc cgccgcgaat cagcccctgc
      481 gagcggagca gctccacgtc ccggtgcatg acgaacgtac gggccggggc gatctcgttc
      541 aggaacacct cgggggagat gtcgaacgag gccacctgcg taccgaccgc cgggctgtcg
      601 tactcgatcg tgaaggagat gcgcagcccc tcgtgaggaa tgccgatcaa ctccactccc
      661 ccgcgattca gcgaaaccgg tccggggatg cgcgcccagc gcttctcggc cgactgttcc
      721 acgatgccgg caccgagcag cgccctggcg atcggcaggg ccgacccgtc ggtcggctcg
      781 gccgcctcct gcccgtcgat ctcgatgacc aggttgtcga cgccgagccc cgccacggcc
      841 gccaggacat gctccacggt gtggacctcg gccccgttgc tgcggaggat ggtgcggcgg
      901 ccggcgcccg ggtcgtacgt ggccgattcc ggccggaccg ggatcttcgg gcgccccggc
      961 aggtcgaccc gttcgaagac gacgcccgag ttgggtgcgg ccggccggaa gttgagcgtg
     1021 gtcggttgcc cgctgtggag cccgacgccg gagagcgtgg ccggtccgcc gatggtgcgc
     1081 tgctggctca cagcacccgc tcccgttctt tttcgagttc acgctccagc tcccccagcc
     1141 gcgcggtcag cgcccggatc tcgcgcacga gttcggggag ccggcgggtg tgcgcctgta
     1201 cgcggagcgc ctgctcgtgg tcggaggccg gataaccgga aacgcgcgcg tcgtcgggga
     1261 ccgacttggt gactcccgcc tgcccgccga tcatcgaccg gtcgccgatc ttcaggtggc
     1321 cgccgacgcc ggcctgcccg cccagggtga catcgccgcc gatgtgcgtg gagccggaaa
     1381 cccccacctg cgcgcagacg atcgaatgcg gcccgatcgt gacgttgtgg gcgatctgga
     1441 cgagattgtc gatcttgaca cccgccccga tgtgcgtgac gcccgtggtg gcccggtcga
     1501 tcgtggcgtt ggcgccgatc tcgacgtcgt cgtcgacccg gacggtgccg agctgcggca
     1561 ccttgagctg caccccaccg gccttcacgt agccgaaccc gtcggcgccg atgaccgcgc
     1621 ccggatggat gatcacccgg ttgccgatgg tgatggcgtc gcgcaggacg acgttggggt
     1681 agacggtgac gtcatcgccc aaccggcagt cggcgccgac gtagacgccg gccatgagcg
     1741 tgacgcgctc gcccagcgtg gcgccggact cgaccaccac gttcgggccg atggaggcgc
     1801 cggcgcccat cgaggcgccc gcgtcgacca ctgcggtggg atggatgccc gcagcgggcc
     1861 tcgggcgctg tccgccgaac aggtggagcg tctgcaggaa gccgacgtac ggatcggtca
     1921 cccggatcag gaccggaccc tccccttggc cccgctcgtg gtcatggccg acgatcaggg
     1981 catgggcccg cgtggaggaa acgtagctgg cgtaacgcgg atgtgccagg aaggcgatct
     2041 cccccggccc ggcctcgcgg atccccgcaa cgccggtgat caggggatcg accgttccgt
     2101 cgagctgtcc gccgatcgcc ttggcgatcg cagacgtcag aaagctgggc acgcgcgcct
     2161 cctccgaccc gcccgtcgtg gtgggccgcc ctggcggcat cacctggggg tgctggctcc
     2221 ggggttggtc tggttcagtt cgccgataac ccggtcggtg agatccagag ccggatcccc
     2281 gtacaccagg ttgccgtccg cggcgtcgag gatgaacgtg tacccctccg ccgtcgcgat
     2341 ccggagcgtg acatcccgga tcttgagcac gatgtccttg gtcagctgct ggttgcgttg
     2401 ggccaccttg ccggtcggcc cccagatttc ccgctgcagc tgttcgagtt cgctgatgcg
     2461 cgactggagc tcggtttccc gctccttccg ccgggcgtcc gagagcatga gtttctgcgc
     2521 ctcgaactcc ctggttgcct tgtccagttc ggccttcttg ttttcgaact gccgggccca
     2581 cccttccagg tcctggttga attcactttc ggcggacttg gtgccggtgt actcgcggaa
     2641 aaccttctcc gaatcgatgt agccgatctt gtagtcggcg gccagcgcga tggtggcgca
     2701 gtttatcaga gccgccgcca gaatcaaggc ccggaagagt ggtgatgaac gcacaaactg
     2761 tctcctcgtc atgagttatc ggggagcgcc accgcggcgc cggcctagaa gaatcgtccg
     2821 aacaacaggt gcccctgcca accgccacga tcgtaatgcc ctcccctgcc ggggatgatg
     2881 ttgcggttca ggcggtcgaa cccgtaggcg taatcgaagc ccacgagtcc caggaggggg
     2941 atttccatcc gggcgccgat ccccgccgcc ttgtggaggg agtcccagcg gaaatcggag
     3001 aggtcgttcc aggtgcctcc cgcctcggca aagatcaccc cgtggagcgg ttcgacgatg
//
LOCUS       DASVOD010000066         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_839219, whole genome shotgun sequence.
ACCESSION   DASVOD010000066 DASVOD010000000
VERSION     DASVOD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839219"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF720_01295"
     CDS             <1..206
                     /locus_tag="VF720_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006749029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX7878010.1"
                     /translation="PEGFRSATVKPAGAFYSDELREFLLPYDRVRESSDPDRTLLDFL
                     ETTYAAAATLGAWDRNALEKEQL"
     gene            complement(247..1260)
                     /locus_tag="VF720_01300"
     CDS             complement(247..1260)
                     /locus_tag="VF720_01300"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878011.1"
                     /translation="MSPLSDDDLAHLRSVLPEESRYRLIEAIGHGGMGVVWRAHDTRL
                     GRDVALKVLAAHQPGPDAEARLDREASILARLEHPGIVPVHDSGRLEDGRLYYVMKLV
                     QGRLLSAALDEGASPGQRVDVLIRIAEAVAFAHRAGVIHRDLKPSNIMVGEFGEVLVL
                     DWGMAGWLGERSGDDTVPSVSVDAARAVPTDERGGAPPTATGTVLGTPGWMSPEQSRG
                     QRTDARSDIWGLGALLSWSFGGGPTTLGSDGQPEPTPALPRPLRSIARRAQAVDPAAR
                     YADADAFLADLRAWRDGDRVMAHRESALERVGRFVSRYRVPILLIGAYLLVRVAIYVW
                     GRI"
     gene            complement(1260..1985)
                     /locus_tag="VF720_01305"
     CDS             complement(1260..1985)
                     /locus_tag="VF720_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878012.1"
                     /translation="MSDDRFPETRVSAVLAARSHEEAARRAAMDRIIGAYWKPVFKYL
                     RLRWKADHDQAADWTQAFFTRLLEREFVQSWDPERGHFRTWLRVGIDGFVANEKKAAG
                     RQKRGGGRAPLSLDFDGAERELGPLMPMEMLHPERCFEQEWARALFDSGIAALRDDLE
                     SNGRALRWRIFEATDLAHGERPSYRELAGRFREPETQITNHLAAARRQLRQILSQRLR
                     DLTGSDEEYDFEAGRLFGTKERV"
     gene            complement(1982..>2932)
                     /locus_tag="VF720_01310"
     CDS             complement(1982..>2932)
                     /locus_tag="VF720_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012074627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX7878013.1"
                     /translation="HLVHLGSRAVGGAALVMAEATGVEPRGRISPHDLGIWSDAHVPP
                     LARAAAFIREHGAVPAIQLAHAGRKAGTGRPWEGGKRLGLGEGGWIPVAPSPVPFAET
                     DPTPTALDERGIHHIIALFVAAAERSLAAGFEVIEIHGAHGYLINQFLSPLSNLRTDH
                     YGGSFENRVRLLLEIASAVRSVWPERLPLFVRVSATDWAEGGWTDDDTVRLAPLLQER
                     GVDLIDCSSGGVVAGAKIPVGPGYQVPFAERVHKEAGLLSGAVGLITEPAQADAIVRE
                     GRADLVFLARELLRDPYWPLRAAKALGHPAPVPPQYLRAW"
BASE COUNT          482 a          999 c         1017 g          434 t
ORIGIN      
        1 agccggaagg tttccggtcg gcaaccgtga aaccggcggg tgcgttctac agcgacgagc
       61 tccgtgaatt cctgctgccg tatgatcggg tgagggagtc gagcgatccg gacaggacgc
      121 ttctcgattt cctcgagacg acctacgcgg cggccgcgac gctcggcgcg tgggaccgaa
      181 acgcactgga aaaagaacag ctgtgaggtg cggcacggaa gccgccatcc ccgtacggtg
      241 cattaactag atccggcccc acacgtagat cgccacgcgc accaggagat aggccccgat
      301 caacagaatc ggcacgcggt atcggctgac gaatcgtccg acccgctcga gtgccgactc
      361 ccggtgcgcc atcacccggt caccatcgcg ccatgcgcga aggtcggcaa ggaaggcatc
      421 cgcgtcggcg tagcgcgcgg ccggatcgac cgcctgtgcg cggcgggcga tggaacgaag
      481 gggtcggggg agggccggtg tcggttcggg ctggccatcg ctgcccagcg tcgtcgggcc
      541 accgccgaac gaccacgaga gaagcgcgcc aagcccccag atgtcgcttc gggcgtcggt
      601 gcgctgcccg cgggactgtt cgggcgacat ccagccgggt gtaccgagga ccgttcccgt
      661 ggcggtcggg ggggcgccgc cccgctcatc ggtcggcacg gcccgcgcag catcaacgga
      721 aaccgacggc accgtgtcgt cacctgaccg ttcgccaagc caccccgcca tgccccagtc
      781 cagcaccagc acctccccga attcgcccac catgatgttc gatggtttca ggtcacggtg
      841 gatgacgccg gcccggtgcg caaaggccac cgcttcagcg atgcgaatga ggacatccac
      901 tcgctggccg ggggacgcgc cctcatcgag cgctgcggag agaaggcggc cctgcaccag
      961 tttcatcaca tagtagagcc gaccatcttc caggcggccc gaatcatgca cgggaacaat
     1021 gcctgggtgc tcgagacggg caagaatcga ggcctcccga tcgaggcgcg cctcggcgtc
     1081 cggccccggc tgatgcgcgg ccagcacctt gagcgccacg tcgcgcccca gacgggtgtc
     1141 gtgcgcgcgc cagaccacac ccatcccgcc atggccgatc gcctcgatca accgatagcg
     1201 cgactcctcc ggcagcacgg agcgaagatg cgcgaggtcg tcgtcggaga gcgggctcat
     1261 tacacccgtt ccttcgtacc gaagagtcgc ccggcctcga agtcatactc ctcgtcgctg
     1321 cccgtcaggt cgcgcagacg ctgcgagagg atctggcgca gctgtcggcg ggcggcggcg
     1381 agatgattgg tgatctgcgt ctccggttca cggaagcgcc cggccagttc gcgataggac
     1441 ggccgctcgc cgtgtgccag gtcggtggct tcgaagatcc gccagcggag cgcacggccg
     1501 ttcgattcga gatcgtcgcg caacgccgcg atccccgagt cgaacagagc gcgcgcccac
     1561 tcctgctcga agcagcgctc ggggtggagc atctccatcg gcatgagcgg cccgagttcg
     1621 cgctcggcgc cgtcgaaatc gagagagagg ggcgcgcggc ccccaccgcg cttctgccgc
     1681 ccggccgcct tcttctcgtt ggccacgaac ccgtcgatgc cgacgcgcag ccaggttcgg
     1741 aagtggccac gttccggatc ccacgactgg acgaattctc tttcgaggag tcgcgtgaag
     1801 aaggcctgag tccagtcggc ggcctggtca tgatcggcct tccagcggag gcgcaggtac
     1861 ttgaagaccg gcttccagta ggcgccgatg atccgatcca tggccgcgcg gcgcgcggcc
     1921 tcctcgtggc tgcgggcggc caggacggct gagacgcgcg tttctggaaa gcggtcgtcg
     1981 ctcaccaggc gcggaggtac tgcggcggca ccggcgccgg gtgtccgagc gctttggctg
     2041 cgcgcagggg ccagtacgga tcgcgcagca gctcacgcgc gaggaagacg aggtcggccc
     2101 taccctcgcg gacgattgcg tcggcctgcg ccggttcggt gatcaggccg acggcgcccg
     2161 agaggagccc cgcctccttg tggacacgtt cagcgaacgg cacctggtat ccgggtccga
     2221 ccgggatctt ggcgccggcc accacgccgc ccgacgagca atcgatgagg tcgacaccgc
     2281 gttcctggag gaggggcgcg aggcgcaccg tgtcgtcgtc ggtccaccct ccttcggccc
     2341 aatcggtagc cgacacccgg acgaacaggg gaaggcgttc cggccagacg ctgcggacgg
     2401 ccgacgcgat ctcgaggagc aggcgcaccc tgttttcgaa tgacccgccg taatgatccg
     2461 ttctcaggtt cgagaggggg gagagaaact ggttgatcag gtagccgtgc gcgccgtgga
     2521 tctcgatcac ttcgaagccg gccgcgaggc tccgttcggc cgcggccacg aagagcgcaa
     2581 tgatatggtg gatgccgcgt tcatcgagcg cggtcggggt cgggtcggtt tcggcgaacg
     2641 gaacggggga gggggctacc ggaatccagc ctccttcacc gagcccgagc cgctttcctc
     2701 cttcccaggg gcggccggtg ccggccttgc ggccggcgtg ggccagttgg atggccggta
     2761 cggcgccgtg ctcccggatg aaagccgccg cgcgcgccag gggaggcacg tgggcatcgc
     2821 tccatatccc cagatcgtgc gggctgatcc ggccgcgcgg ctccacgccc gtggcctcgg
     2881 ccatgaccag cgccgcgccg ccgacggccc ggctacccag gtgcaccagg tg
//
LOCUS       DASVOD010000067         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_734977, whole genome shotgun sequence.
ACCESSION   DASVOD010000067 DASVOD010000000
VERSION     DASVOD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734977"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="VF720_01315"
     CDS             <1..960
                     /locus_tag="VF720_01315"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878014.1"
                     /translation="DITDTGTFVHRDTVEVTVGTPLVVMSANGTSLAGWTVTGAWGLQ
                     TVSGNSVFSDSPSGKYANGISTTLTRTADLDLSGGANPVLVFRTLYDFERGYDCGRVE
                     VSTNGGGSWTILRGRLNRPGKGNTGAYGGGTQELGQPIYDGTQRIWGDELIDLSAYQG
                     LTNVRLRFRVTSDSGLNRDGWKIDDIAVRSYPVDVAGVADGARPGGGQGGGQGGGQGG
                     GLELHPRLMAVTPNPVGHSGAQLRADFPAATAYRLVVYDVLGRLVQVVSEGITAAGQR
                     EFWWRPDATADGARGVVDGPASVYYLALEWSGGRESRAVVRVE"
     gene            complement(1116..>1522)
                     /locus_tag="VF720_01320"
     CDS             complement(1116..>1522)
                     /locus_tag="VF720_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HEX7878015.1"
                     /translation="TLPYLLRAFQLRFVARLGHGMELHSCLGCSRSIGADGGRGIGIA
                     DAGRLVLSASQGGLFCADCAGDADGTVAITPAALAVLRAYLEQDLDVAIRQRLAPAVR
                     AETGRIIEAFLAAQFDHYRGLRSLKLAASALG"
BASE COUNT          246 a          504 c          553 g          219 t
ORIGIN      
        1 gacatcaccg acaccggcac gttcgtccac cgcgataccg tggaggtgac cgtcggcacg
       61 cccctcgtcg tgatgagcgc caacggcacg tcactggccg gctggacggt caccggcgcc
      121 tggggcctcc agaccgtcag cggcaacagc gtcttttccg actcgccttc cggcaagtac
      181 gccaacggca tctcgacgac gttgacccgg acggccgacc tcgacctgtc gggcggcgcg
      241 aaccccgtgc tcgtcttccg cacgctgtac gacttcgagc gcggctacga ctgcgggcgc
      301 gtggaagtgt cgacgaacgg cggcggaagc tggacgatcc tcagggggcg actaaaccgc
      361 ccgggcaagg gaaacaccgg agcctacggc ggcggtacgc aggagctcgg ccagcccatc
      421 tatgacggca cgcagcggat ctggggcgac gagctcatcg acctctcggc ttaccaggga
      481 ctcaccaacg tgcggctccg gttccgcgtc accagcgatt ccggcctcaa ccgcgacggc
      541 tggaagatcg acgacattgc cgtgcggagc tacccggtgg atgtggcggg cgtggcggat
      601 ggtgcgcgac ccggtggcgg ccagggtggc ggccagggtg gcggccaggg tggcggcctc
      661 gaactgcatc cgcgtctgat ggccgtgacg ccgaatcccg tggggcactc cggtgcgcag
      721 ctccgcgccg acttccccgc cgcgacggcc tatcgacttg tggtgtacga cgtactgggg
      781 cggctggtgc aggtcgtgtc cgaggggatc acggccgcgg gtcaacggga attctggtgg
      841 cggcccgatg ccaccgctga cggcgcccgt ggcgtagtgg acggccccgc ctcggtttat
      901 tacctggctc tcgaatggag tgggggacgg gaatcccggg ccgtcgtgcg ggtggagtag
      961 cgtggcgggc gccatgatcg gcagcggtca cctgtttgcg atagagacgg aactcgcacc
     1021 ggtgaaagta agctgtcgag gtgtggcccg acgcgcttgt gaaaaggggt gcaactcgca
     1081 ccggtgaaag tgccgcgccc ccgcggcagc tctccttacc ccagcgccga cgccgcgagc
     1141 ttcaatgacc gcaggccgcg gtaatgatcg aactgcgccg ccaggaaggc ctcgatgatc
     1201 cggccggttt cggcgcggac cgccggggcc aggcgctggc ggatggcaac atcgaggtcc
     1261 tgctcgaggt aggcgcggag cacggccagc gccgccgggg tgatggccac ggtgccgtcg
     1321 gcgtcccccg cgcagtcggc gcagaagaga cccccctggg aggcggagag aaccagtcgg
     1381 ccggcatccg cgattccgat cccccgtccc ccgtcggcgc cgatcgagcg ggagcatcca
     1441 aggcaggagt gcagctccat cccgtggccc agccgggcaa cgaatcggag ctggaaggcc
     1501 cgcagcaggt aggggagcgt ct
//
LOCUS       DASVOD010000068         4535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_996103, whole genome shotgun sequence.
ACCESSION   DASVOD010000068 DASVOD010000000
VERSION     DASVOD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996103"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="VF720_01325"
     CDS             complement(<1..168)
                     /locus_tag="VF720_01325"
                     /inference="COORDINATES: protein motif:HMM:NF037181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878016.1"
                     /translation="MTATRSVFVCQSCAAEHPRWFGKCPSCDAWNSLTEEIRRKPVKK
                     PGRSVGGITGLG"
     gene            complement(168..440)
                     /locus_tag="VF720_01330"
     CDS             complement(168..440)
                     /locus_tag="VF720_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878017.1"
                     /translation="MTERQLTIERDRQGAPLVAYLYLPAPNGRKSAQCHEVEPGYVVD
                     IDSAGVLIGVEFFYPDEVQLAILDRILEEHHAPPVTPADLAPLKVA"
     gene            complement(975..1232)
                     /locus_tag="VF720_01335"
     CDS             complement(975..1232)
                     /locus_tag="VF720_01335"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX7878018.1"
                     /translation="MRISYYADGRPMIAYLYLPGRKGVKSARSREVEIGYVLDFDIEG
                     VVIGIELLYPDEVTLEAINRILTQYHATPVTPADLAPLKVA"
     gene            complement(1240..1515)
                     /locus_tag="VF720_01340"
     CDS             complement(1240..1515)
                     /locus_tag="VF720_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878019.1"
                     /translation="MVAPRKRPEWWDWELKILAHAELSLDQRDLTELDLRQMFWKADR
                     TSPDPRRGRFRVDVFHRSQRWVIVVEPVIEERQLVVLTIYEVDRLGE"
     gene            complement(1603..2508)
                     /locus_tag="VF720_01345"
     CDS             complement(1603..2508)
                     /locus_tag="VF720_01345"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX7878020.1"
                     /translation="MKRYNDLTIGITVLAAIALLIFGVLWFKGFTTSRETWKLAAYFP
                     QSSGLADGDPVEVAGVVQGKVDDIIYERGRAKLEMSMSQGAELYADATIAIANYGMMG
                     QKFIAIDPGNPALGPLDTSKPMEGEFQPGVGDMMTEVGQSLAATNLLVDRLNRFLAVV
                     DSTGGALSIGHTITHLETITENVSTLTSENRVRLAMSVANFEAASGELRSLLSEKGPT
                     LRNTFDNLERTTLQADTTARELKAMATELRTLISKINSDSSTAGALLTDRALYNRLLS
                     AADRTDSLLIDVQRNPRKYIKFSVF"
     gene            complement(2531..3307)
                     /locus_tag="VF720_01350"
     CDS             complement(2531..3307)
                     /locus_tag="VF720_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878021.1"
                     /translation="MIRCEGLWKRFGAKEVLRGVDLEIPDGQALTIIGRSGTGKSVLL
                     KHIIGLIPPDKGRVLVDGQDLSVLKYDELNDVRRRFGMLFQMAALFDSMTVGENVGLG
                     LREHTKMPEAEVKARVAEKLAMVGLSGIEDLKPASLSGGMRKRVGLARAIAMEPRCVL
                     YDEPTTGLDPVTADTINVLIKDLQQKLGITSIVVTHDMKSAAYVSDRIAMLHEGHLIF
                     DGTFEEIEASPDPIVQQFITGSSHGPLTDEAPVVTARRKR"
     gene            complement(3304..4104)
                     /locus_tag="VF720_01355"
     CDS             complement(3304..4104)
                     /locus_tag="VF720_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878022.1"
                     /translation="MAAREIGAGFVGLATAILDIISEVGRFSRFLGLLIAQTIPGLKY
                     WHLFMDQCRAIGIGSLPLVTLTSLFVGAVAALQAAYQFKNNVPLLWLGSVVAKSVVIE
                     VGPVLTALVVGGRVGASIAAELGTMKVTEQIDALETLALSPVRYLAVPRLLAATLMVP
                     VITVFADALAIFGGMVVAVFRVGMTPHLYFKGMKLFFEVEDIYGGLIKSFVFGSIIAI
                     MGCYYGMRTEGGAEGVGIATTRAVVASCVMILISDYFLAEVIFQVLFR"
     gene            complement(4104..>4535)
                     /locus_tag="VF720_01360"
     CDS             complement(4104..>4535)
                     /locus_tag="VF720_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002707688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7878023.1"
                     /translation="EQPTGLELACGTYDDVSVLDMWNGTATSIWDEIVSITPAGEEEV
                     FDLTVPGPASWLADGIVSHNSGALEQDADVVMFVYRPAMYDKTDENENMAEIIVAKQR
                     NGPTDTINLTFLRDQTRFASMDRRHGGEPFAAPAGLEEPYA"
BASE COUNT          848 a         1411 c         1445 g          831 t
ORIGIN      
        1 gccgaggccg gtgatgccac ccacgctacg gcccggcttc ttcacgggct tgcggcggat
       61 ttcctcggtg aggctgttcc aggcgtcgca cgacgggcac ttgccgaacc agcgcgggtg
      121 ttcagcggcg cacgactggc agacgaatac ggagcgcgtt gcggtcacta ggccactttc
      181 aggggggcga ggtcggcggg ggtgacagga ggggcgtggt gctcctccag gatgcgatca
      241 aggatggcta gctgcacttc atccggatag aagaactcga cgccgatcag gactcccgcc
      301 gagtcgatgt ccaccacata ccctggctcg acttcgtggc attgcgcgct cttcctccca
      361 ttcggagccg ggagatagag gtacgccacg agcggggcac cttgcctgtc tctctcgatg
      421 gtaagttgtc tctcagtcat tgcttgccct gatatcgaag attgtcccga ttcctcggcg
      481 caccacccgt ccttcgggac aggaggacca gggaagtcta gcaacagtcc gcgtcgagcg
      541 gtcaatgctg acgaacccgt aggccagaac acaagcgatc ggtggcacag gattgccctg
      601 agctaaccga gcgtgtcgcg gtcgctagtt cactttcagg ggagccgggt cggctggtgt
      661 gacagggggc tccccgtatc gggtgagaat agaatcgacc tttttcaagg tcacggcttc
      721 aggatccaac agctcaattc cgagcaatgc gcctgctgca tcgagatcga tgatgtaatt
      781 cggaaagatt ccttcgaccg gaaatatcgg gcggcccgcg tgtcgctcaa gtagagatag
      841 gcggccatgg gccggccgtc cttgtagtag ccaatcttga gatatggata gttcaccatg
      901 tcgtgttcat gccgacccgc cgcaatgcgc tgtggagcac gactgacagc cgaagagcga
      961 ccgtgtcgcg gtgctcaggc cactttcagc ggggcgaggt cggcgggggt gacaggggtg
     1021 gcatggtatt gggtgaggat ccggttgatg gcctccaggg tcacctcgtc cggatagagc
     1081 agctcgatgc caatcacgac gccttcgata tcgaagtcga ggacatagcc aatctcaacc
     1141 tcgcgggacc gtgcactctt cactcccttc ctaccgggaa gatagagata ggcgatcatc
     1201 ggccgcccgt cggcgtagta gctgatcctc aagttcgcct cactcgccaa gtcgatccac
     1261 ctcgtaaata gtgagcacca ccagttgtcg ttcctcgatc accggttcga cgacgatcac
     1321 ccaccgctgc gaccggtgga aaacgtccac cctaaatctt ccgcgtcgtg gatcgggaga
     1381 tgtccggtcc gctttccaga acatctgtcg caggtcgagt tcggtgagat ctcgttgatc
     1441 caaactcagt tcagcgtggg cgaggatttt cagttcccaa tcccaccact ccggccgttt
     1501 cctcggcgcc accacccgtc ctccgggacg ggaggctcgg gatcaagtct agccacagcc
     1561 cgctctgaac cgtcaagccg gaaatcaccg gccgaagtcc ccctagaaca ccgaaaactt
     1621 gatgtacttc cgggggttcc gctgcacgtc aatcagcagc gaatcggtgc gatccgccgc
     1681 cgacaacagc cggttgtaca gagcccgatc ggtgagcaac gcgcccgcgg tcgaactatc
     1741 cgagttgatc ttggagatca atgtgcgcag ttcggtggcc atggccttga gctcacgagc
     1801 cgtggtgtcg gcctggagcg tcgtgcgctc gaggttgtcg aaagtattgc ggagcgtggg
     1861 ccccttctcg gacaggaggg agcggagctc gcccgaggcg gcctcgaagt tggccacgct
     1921 catggccagg cgcacgcggt tctcgctggt cagcgtcgag acgttttcgg tgatcgtctc
     1981 gaggtgggtg atggtgtggc cgatggagag cgcgccaccg gtcgagtcga cgacggccag
     2041 gaagcggttc agtcgatcga cgagcaggtt ggtggccgcg aggctctgcc ccacctcggt
     2101 catcatgtcc ccgacgccgg gctggaattc cccctccatc ggtttcgatg tatcgagcgg
     2161 accaagggcc ggatttcccg ggtcgatcgc gatgaatttc tgccccatca tgccgtagtt
     2221 ggcgatggcg atcgtggcgt cggcgtagag ctcggcgccc tgggacatcg acatctcgag
     2281 cttggcgcga ccgcgctcgt agatgatgtc atccaccttt ccctgcacga caccggccac
     2341 ttccaccggg tcaccgtcgg ccagccccga ggactgggga aagtacgcgg cgagcttcca
     2401 ggtttcgcgg ctcgtggtga aacccttgaa ccagagcacg ccgaaaatca ggagtgcgat
     2461 ggccgccagg acggtgatgc cgatggtcag gtcgttgtaa cgcttcattc actccccccg
     2521 gcggaacggc tcaccgtttc cgccgcgcgg tgacgaccgg ggcctcgtcg gtcagcggac
     2581 cgtgcgagga accggtgatg aactgctgga cgatcggatc gggactggcc tcgatctcct
     2641 cgaaggtgcc gtcgaagatc aggtgcccct cgtgaagcat ggcaatgcgg tccgacacgt
     2701 aggccgcact cttcatgtcg tgcgtgacga cgatcgaggt gatccccagt ttctgctgca
     2761 ggtccttgat cagcacgttg atggtgtcgg ccgtcaccgg atcgagcccc gtggtgggct
     2821 catcgtacag cacgcagcgg ggttccatgg cgatggcccg ggcgaggccc acgcgcttgc
     2881 gcatgccgcc ggacaggctg gccggcttca ggtcttcgat gcccgacaaa cccaccatgg
     2941 ccagtttctc ggccacgcgg gctttcacct cggcctcagg catcttcgtg tgttcgcgca
     3001 gccccaggcc cacgttttcg ccgacggtca tcgagtcgaa aagcgccgcc atctggaaga
     3061 gcatcccaaa gcggcgtcgc acgtcgttca actcgtcgta cttgaggacc gacagatcct
     3121 ggccatccac caggacccgc cccttgtccg gcggaatcag gccgatgatg tgcttgagaa
     3181 gcacgctctt cccggtgccg gagcgcccga tgatcgtgag cgcctggccg tcggggatct
     3241 cgagatccac gccccggagc acttccttgg cgccgaaccg tttccagagc ccctcgcaac
     3301 ggatcaacgg aagagcacct ggaagatcac ctcggcgagg aagtagtcgg agatgaggat
     3361 catgacgcag gaggccacga ccgcccgcgt cgtggcgatg ccgacgcctt cggcgccacc
     3421 ctcggtgcgc atgccgtagt agcaccccat gatcgcgatg atcgaaccga agacgaacga
     3481 cttgatgaga ccgccgtaga tgtcttcgac ctcgaagaag agcttcatcc ccttgaagta
     3541 gagatgcggc gtcatgccga cgcggaagac ggccacgacc atgccgccga agatggccag
     3601 ggcgtcggcg aagacggtga tgaccggcac catgagcgtg gcggccaaca ggcgcggcac
     3661 ggccaggtag cgcacgggcg agagggccag cgtttccagc gcgtcgatct gctcggtcac
     3721 cttcatggtg ccgagctcgg ccgcgatgga ggcaccgacg cggccaccca cgacgagcgc
     3781 cgtgagcacc ggccccacct cgatgacgac cgacttggcc accaccgaac cgagccagag
     3841 gagcggcacg ttgttcttga actggtaagc cgcctgcagc gccgccacgg cgccgacgaa
     3901 gagcgaggtg agcgtgacga gcggcaggct gccgatgccg atggcccgac actggtccat
     3961 gaagagatgc cagtacttga ggcccggaat ggtctgcgca atcagcaggc cgaggaaacg
     4021 cgagaagcgc ccgacctcgg aaatgatatc gaggatggcg gtggccagcc cgacgaaccc
     4081 ggcgccgatc tcgcgggccg ccatcaggcg tacggttctt ccaaccccgc cggcgccgcg
     4141 aacggctccc cgccgtgccg ccggtccatc gaggcgaacc gtgtctggtc gcggaggaac
     4201 gtcaggttga tcgtgtccgt cggaccatta cgctgcttgg ccacgatgat ctccgccatg
     4261 ttctcgttct cgtccgtctt gtcgtacatg gcgggacggt agacgaacat gacgacgtcg
     4321 gcgtcctgtt cgagggcgcc ggaattgtgg ctgacgattc cgtcggcgag ccacgacgcg
     4381 ggaccgggga cagtgaggtc gaagacctct tcctcgccgg cgggagtgat ggagacgatt
     4441 tcgtcccaga ttgaggtagc agtcccattc cacatgtcca gtactgagac atcgtcatat
     4501 gtgccgcaag ccaattccaa gcctgtcggt tgctc
//
LOCUS       DASVOD010000069         6407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_161576, whole genome shotgun sequence.
ACCESSION   DASVOD010000069 DASVOD010000000
VERSION     DASVOD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161576"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VF720_01365"
     CDS             complement(<1..1799)
                     /locus_tag="VF720_01365"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEX7878024.1"
                     /translation="MASPLPARKTASSRLHWLALLIPLGYLPSYLFGSRPGALDTIAI
                     VAATAAAWFLVAWSARQPMFTGVTGAPTRTPKKFGATLAWSIGLLAAAVLFTLIIGPA
                     AAGRAEAFGRKEAWSVGIFQQALWWSGHGVPLGATYGTADGSLHSQFGIHFSPFFLAF
                     EPIYRWRPEAVTLFGLQALFLGLAALPLFALARRKVGAGGAGVVALAWLLQPMVIGAP
                     LTGFHDLSFAPFFIFTAFWAMSSERGFLYLAATLLLMSIREDLAIFVIAMGFAGMFLK
                     APGRYVMGSIVLGVAWLVVTTIGVMPGYRTGTLLAGPDVFLQQYLGSWGATPTAIVTN
                     MLTHPVTFLATLTTHEALLYLLTMLRPTGFLLPLADPSWVAALPTAMLNMASEGANLK
                     MPLGRYSLPVIAALIAAVPGAMAWWGRRLGAPAAPAVDTALLGRNMEERAAFLRAESR
                     LGSHWKAAERDEFSLEVGRRGAPLAWLVVMAALLALTIMRIGTQFRSEVSPANAAQAG
                     AVAAVPVDAGVLAPDWAYSKLANRALFASLGSLENRALDARLLARFDAVIVDMSPGSF
                     EMTKYPTLVPALLMRMRGDRAFTELTTADGIST"
     gene            1943..3307
                     /locus_tag="VF720_01370"
     CDS             1943..3307
                     /locus_tag="VF720_01370"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX7878025.1"
                     /translation="MTWIDENADRFVAELTEFLRIPTVSAVPIHAPDVVRGAEWIAAH
                     LERIGLTNVGIHPTPGHPIVYAEWLGAGPDAPTALVYGHYDVQPVDPLELWRHPPFEP
                     TREGVNLVARGATDDKGQVFTHVKAAEAWLHTTGRLPINVKYLIEGEEEVGSEHLDAW
                     VASNADRLRCDVVVISDGSQYGHGQPAINYGLRGLAYFEIRVDGPNRDLHSGAFGGAV
                     ANPANVLCRLLGELLDADGRITIPGFYDDVRPLADTERAAWRALPFDETSFCDAIGIP
                     ATTGEAGFSNQERRWARPTCDVNGLFGGYQGEGAKTVLPAWAGAKLSFRLVPDQAPKR
                     VGELLRRHLAARAPAGVRVTVTDLHGGMPVLVPHDGPAIAAGRRAIERGFGKPPVLTR
                     EGGSIPVVETFQRVLGAPTLLIGYGQKDDNTHSPNEKFNMDDFQRGIRTSAALLEELA
                     ELKR"
     gene            complement(3340..3975)
                     /locus_tag="VF720_01375"
     CDS             complement(3340..3975)
                     /locus_tag="VF720_01375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX7878026.1"
                     /translation="MSGIVYPEIESYLAPLSTSRHEVLVEMEARAAAENIPIVGPLVG
                     AFLFQMVSLRGARRIFELGSAIGYSTIWLALAAGPDAEIFYTDGDEAKAEDAARYFER
                     AGVSDRITIKVGDALDMFDATGGDFDMIFNDVDKHDYPRVLEKALPRVRKGGLLVTDN
                     VLWSGRVAHLTSEDDRETPAIQQYNELAASTPGCVYSIMPLRDGVGVLLKS"
     gene            4234..4665
                     /locus_tag="VF720_01380"
     CDS             4234..4665
                     /locus_tag="VF720_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7878027.1"
                     /translation="MNWQNPDDETVIDLLSRVRSIVVVGLSPKPARDSHSVSAYMQRQ
                     GYRITGVNPGHRTILGEPCYPSLAEVPADRPLELVNFFRNAALVGRDMEAAVSAGARA
                     LWTQYDVIDEEAARRATDAGVLVIMDRCILVEHRRLIGGGR"
     gene            5158..6366
                     /locus_tag="VF720_01385"
     CDS             5158..6366
                     /locus_tag="VF720_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878028.1"
                     /translation="MSHATTSVFIAHLAVFDARELYLGEGCSSIFTYCTRVLHFSESA
                     AQNRITVARLVQRFPAVLDGLGDGSLTLTALRLLSTHLRTDNAAELLGLARHASKHDI
                     QKLIATLAPRADVAFSIRRLSSDRTEQPFPPGVTIGLPEGAKENVDGVGIANTVGAAV
                     SDPAANVVNASADSVEAGVGNAGAHIHPADESRSASAQLGSTSVQRTDPGQTVPLSHR
                     LALRIVPLSPGRYRLQFTAGESFIARLDEARNLCRHQVPDGDRTTILEMALVRYVEFL
                     RQRRFGGTERKRGGKSPAAAPSAGGEPPVLSGLPVELPAQHHRRKGRSSHRSKRRIAV
                     AVRREVWRRDEGRCTFHGPDGMRCNESGFVEFHHVVPFALGGSATMENIVLRCRAHNQ
                     YEGRLVFGPK"
BASE COUNT         1163 a         2056 c         2122 g         1066 t
ORIGIN      
        1 aaggtcgaga tgccgtcggc ggtcgtcagc tccgtgaagg cccggtcccc acgcatcctc
       61 atgagcagcg ccggcacgag tgtcgggtac ttcgtcatct cgaatgatcc gggcgacatg
      121 tcgacgatca ccgcgtcgaa ccgagccagc agcctggcat ccagcgcgcg gttctcgagg
      181 ctgccgagcg aggcgaagag ggcgcgattg gccagcttcg agtaggccca gtcgggcgcc
      241 agcacgcccg catccaccgg aacggcggcc accgcgcccg cctgtgcggc gttggccggc
      301 gacacctccg aacggaactg ggtgccgatc cgcatgatcg tcagcgcgag gagggcggcc
      361 atcacgacca gccacgccag gggcgcgccg cgccgaccca cctccagcga gaattcatcc
      421 cgctccgcgg ctttccagtg gcttcccagg cgcgactccg cgcggaggaa agcggcgcgc
      481 tcttccatgt tacggcccag caatgcggta tccacggccg gtgcggccgg tgcgccgagt
      541 ctgcgtcccc accaggccat tgcgcccggc accgcggcga tgagcgccgc gatcaccgga
      601 agggagtagc ggccgagcgg catcttgagg ttggctccct cggaagccat gttcagcatc
      661 gcggtcggca acgcggccac ccagctggga tcggccagcg gcaggaggaa gccggtcggc
      721 cggagcatgg tcaggagata gagcaacgcc tcgtgcgtgg taagcgtggc caggaacgtc
      781 accggatgcg tcagcatgtt cgtgacgatg gcggtcggcg tcgcgcccca gcttcccagg
      841 tattgctgca ggaagacatc aggtccggcc aggagcgtgc ccgtgcggta ccccggcatc
      901 acgccgatgg tggtcaccac gagccaggcg acgccgagca cgatcgagcc catgacgtaa
      961 cggccgggcg ccttcaggaa catccccgcg aaacccatgg cgatgacaaa aatggcgagg
     1021 tcttcgcgaa tcgacatgag gagcagcgtg gcggccaggt agaggaagcc gcgctcggac
     1081 gacatcgccc agaaggccgt gaaaatgaag aagggggcga aggagagatc gtggaatccg
     1141 gtgagcgggg cgccgatcac catcggctgc aggagccacg ccagcgccac gacacccgct
     1201 ccgccggcac ccaccttccg acgggcgagc gcgaagagcg ggagtgccgc aaggcccagg
     1261 aagagggcct gtaatccgaa cagggtgacc gcctccggcc gccaccggta gatcggttcg
     1321 aaggccagga aaaatggcga gaagtggatg ccgaactggc tgtgaaggct gccgtcggcc
     1381 gtcccgtagg tggccccgag cggcacgcca tgcccgctcc accagagcgc ctgctggaag
     1441 atccccaccg accaggcttc cttgcggccg aaggcctccg cgcggccggc cgcggcgggt
     1501 ccgataatga gcgtgaagag taccgcggcg gcaagcaatc cgatcgacca ggcgagcgtg
     1561 gcgccgaact tcttcggcgt gcgggtggga gcccccgtca cgccggtgaa catcggctgg
     1621 cgggccgacc aggccacgag gaaccacgcc gccgcggtgg cggcgacgat ggcgatcgta
     1681 tcgagcgcgc cggggcgcga tccaaacagg taggaaggga gatagccgag gggaatgagg
     1741 agtgcgagcc agtggagtcg tgatgaagcc gtcttccttg ccgggagggg tgaggccatg
     1801 cggcgagagt agcaggcggc cgtggacgcg ggtagtatcc gttcctgccg aaatcactag
     1861 gcgtccgccg caacgaaccg gcgcccgccg ataccgaccg aaccccgccg agttcaaccg
     1921 gagccagccg atgccgccag tcatgacgtg gatcgatgag aacgccgacc gttttgtcgc
     1981 cgagctgacc gagttcctcc gcattcccac cgtgagtgcc gtgcctatcc acgccccgga
     2041 cgtggtgcgg ggggccgagt ggatcgcggc gcacctggaa cggatcggat tgacgaacgt
     2101 cggaattcac ccgacacccg gtcatcccat cgtttacgcc gaatggctcg gcgccggccc
     2161 ggatgccccc acggccctgg tttatggtca ctacgacgtg cagccggtcg atcccctcga
     2221 gttgtggcgt caccccccgt tcgaacccac gcgtgagggg gtgaacctcg tggcccgcgg
     2281 cgccaccgat gacaaagggc aggtgttcac gcatgtgaaa gcggcggaag catggctcca
     2341 cacgacaggg cgtctgccga tcaacgtgaa gtacctcatc gagggagaag aggaggtggg
     2401 gagcgagcac ctggacgcct gggtggcctc caacgccgat cggctccggt gcgacgtggt
     2461 ggtgatctcg gatggcagcc agtacgggca tgggcagccg gcgatcaatt atgggctgcg
     2521 cgggttggcc tatttcgaaa tcagggtcga cgggcccaac cgggacctgc actcgggggc
     2581 cttcggcggc gcagttgcca atccggccaa cgtcctctgc cggctgctgg gcgagctgct
     2641 cgatgcggat ggccggatca ccattcccgg tttctacgat gacgtccggc ccctggccga
     2701 caccgagcga gcggcctggc gggcccttcc ctttgacgag accagcttct gcgacgcgat
     2761 cggcatcccg gccacgaccg gcgaggccgg tttctcgaac caggagcgcc gatgggcgcg
     2821 gccgacctgt gacgtgaacg ggttgttcgg cggttaccag ggggagggag ccaagaccgt
     2881 cctgccggcc tgggccggcg ccaagctgtc gttccggctc gtgcccgacc aggccccgaa
     2941 acgtgtcggc gagctgttgc gccgtcatct ggccgcccgt gcacccgcgg gggtgcgggt
     3001 cacggttacc gatctgcacg gtggaatgcc cgtcctggta ccgcacgacg gtccggccat
     3061 cgctgccggc cggcgggcca tcgagcgggg attcggcaag cctcccgtcc tcacccgcga
     3121 ggggggctcg attcccgttg tcgagacctt ccagcgcgtg ctcggcgccc cgaccctgtt
     3181 gatcggttac ggtcagaagg acgacaacac ccactcaccc aacgagaagt tcaacatgga
     3241 cgacttccag cgcggcatcc gcaccagcgc ggcgctgctg gaggaactgg cggagttgaa
     3301 gcggtagggg ggcggcgggc cggatggccc cgcccggcat cagctcttca gcaggacgcc
     3361 cacgccatcg cgcagcggca tgatgctgta cacgcagcct ggggtggacg ctgccaattc
     3421 gttgtactgc tggatggcgg gcgtttcacg atcgtcttcc gacgtgaggt gggccacgcg
     3481 tcccgaccag agcacgttgt ccgtgaccag caggccacct ttccggacgc gcggcaacgc
     3541 cttctcgagc acccgcgggt agtcatgctt gtcgacgtca ttgaagatca tgtcgaagtc
     3601 gccgccggtg gcgtcgaaca tgtccagggc atcgcccacc ttgatggtga ttcgatccga
     3661 cacgcccgcc cgctcgaaat agcgggcggc atcctccgcc ttcgcctcgt ctccatcggt
     3721 gtagaaaatc tcggcgtccg ggccggcggc cagcgccagc cagatcgtcg aatagccgat
     3781 cgcggaacca agttcgaaga tgcgcctggc cccccggagg gaaaccatct ggaagaggaa
     3841 cgcgcccacg agcgggccca cgatggggat gttttcggcc gcggcccggg cctccatctc
     3901 cacgagcact tcatggcggg acgtgctcag tggggccagg tagctctcga tctcggggta
     3961 gacgatgccg gacacgctgt cggattctcc ttgtgaatgg cggcaccggc gccgatccgg
     4021 cgcggcttcc gcatcggaag tcgagcgggg aagtgtgacg gatcgtcgtt acgggtgcaa
     4081 gcgcgggtgc ggaatgagtg ccgcggaagc gatggcactc ggcggcggga accggctgcg
     4141 ctcacaggaa cggctgcggt cggaggtgtg gttgcagtcg gaagtgtggc ggcggtcgga
     4201 ggaacggcag cactcggacg tacgggagac agcatgaact ggcagaaccc tgatgacgaa
     4261 acagtcattg acttgttgag ccgagtccgt tccattgtcg tcgtcgggtt gtcgccaaaa
     4321 cctgctcgcg acagccactc cgtgagcgcc tacatgcaac gccagggcta ccgcatcacc
     4381 ggagtaaatc caggtcaccg caccatcctc ggcgagccct gctatccctc cctggcggaa
     4441 gttcccgctg accgcccact ggaactcgtc aatttcttcc gcaatgccgc cctcgtgggt
     4501 cgggacatgg aggccgccgt ctccgccggg gcccgcgcgc tctggacaca gtacgacgtt
     4561 atcgacgagg aggccgcccg ccgcgcgacc gacgccggag tgctggtgat catggatcga
     4621 tgcattttgg tggagcaccg gcgtttgatc ggtggggggc ggtgagttga caaacggggc
     4681 ttcgaaccct gaggagacag ctgtcgatga atgctccact ctcagcgaac ctgttccgga
     4741 acaggtaaca aatcattcat ggctgagcga gctgttcttg cctgcgtgaa cctgttccgg
     4801 aacaggtagc aaagtcattc actggctgag cgagctggtc ttctctgcgt gaacctgttc
     4861 cggaacaggt agcaaagtca ttcactggct gagcgagctg ttcttccctg cgtgaacctg
     4921 ttccggaaca ggtgactgga agagaccgca cgaattgttg ccgatcgcac ctggcgcatg
     4981 attttcagcc ttgactgttc gaatggaagt tcggcactgt caatcggaca tccgctccgg
     5041 tgaaatcgag gcccgtccgg actcgaagga tgggccatga tcccggaaca cttccttaca
     5101 ccccccgatt cgctctcgag ccctgttctg gcttgccggt tagtcgagct cgcaagattg
     5161 agccatgcaa ccacgtccgt gttcatcgca caccttgccg tgttcgatgc ccgtgagctt
     5221 tacctgggag agggttgctc ctccatcttc acctactgca ctcgcgtcct gcatttctcg
     5281 gagtcggcgg cacagaaccg gattaccgtc gcaaggctgg ttcaacggtt tcccgcggtg
     5341 ctcgatgggc tcggcgacgg gtcgttgact ctcacagcgc ttcgcttgtt gtcgacgcat
     5401 ctgcggaccg acaacgcagc tgagttgctc gggcttgcac gtcatgcttc caagcatgac
     5461 attcagaaac tgatcgcgac gctggcaccc cgggctgacg ttgcgttctc gatccgccgc
     5521 ctctcttctg acagaaccga gcagcctttc ccaccagggg taactatcgg gttgcctgaa
     5581 ggtgcgaagg agaacgtgga tggagtcggc atcgcgaata ccgtgggtgc cgccgtgtcc
     5641 gacccagctg ccaacgtcgt caacgccagc gcagactcag tggaagctgg agttggaaat
     5701 gcgggcgcac acattcatcc tgccgatgaa tcccggtctg cttccgctca actcggctcg
     5761 acctcggtac aacggacaga tcccgggcag accgtgcctc tcagtcatcg gctggcgctg
     5821 cgcatcgtgc cactctcgcc gggccgttac aggctgcaat tcacggcggg agagtcattc
     5881 atcgcccgac ttgacgaggc gcgaaatctt tgtcgccacc aggtacccga cggtgaccgc
     5941 acgacaatcc tcgaaatggc gttggttcgc tacgtcgagt ttctccgaca gagacgattt
     6001 ggaggaacgg agcgcaaacg aggaggcaaa tcaccagccg cggctccctc cgcgggcggc
     6061 gaaccgcctg tgctttccgg gctcccagtg gaacttcccg cgcagcacca tcgccgcaag
     6121 ggaaggtcct cccatcgctc gaagcgccga atcgccgttg ctgttcgtcg tgaagtctgg
     6181 aggcgcgacg aaggccggtg cacctttcac ggacccgacg gcatgcgatg caatgagtca
     6241 ggattcgttg agtttcatca cgtggtgcca ttcgcgctgg gcggatcggc aacgatggag
     6301 aacatcgtcc tgcgctgtcg tgcgcacaat cagtacgaag gtcgcctcgt attcgggcca
     6361 aaatgatcgg acactcgcgc tccgcaagcc ctacccccgc ccccgcg
//
LOCUS       DASVOD010000070         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_682886, whole genome shotgun sequence.
ACCESSION   DASVOD010000070 DASVOD010000000
VERSION     DASVOD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682886"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /locus_tag="VF720_01390"
     CDS             <1..1734
                     /locus_tag="VF720_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7878029.1"
                     /translation="RVFVSNGAAVDSSDAFFSICQSLASGTLYPSTGTGIGMATGDFN
                     EDGITDVAEGGCGVRIHLGLGSGGVGNGAFGPASVVPLVGCGTAVATGDFNDDGITDL
                     VAIDGSGLSTLRGQGSGGTGNGSFVRTATYPFPSGPEGLAVADFNEDGIEDIAAALRL
                     QDQVGILFGNGVSGVGTGTFAPVVLVSTGDGPMDVAVQDFNEDGIWDIATSNAVAANV
                     SILIGLGAGSIGNGTFAAPVHYAGIGGSRFLALDMSNDGIVDLVGGSVTLLKGNGSGG
                     VGNGTFAAAVSSVGLYPILGIMDIDHNTLPDLVGLGAGRLELMRNTSSTGTFESAGWL
                     PSMGANPQFAIAGDFLEDQIIDLAGISHSEPALAVALAGGCVSEAVTITLAEPSPTTW
                     NVGTNQTITWSKSFTNFLNIHGWDVEVSRDNGVNWETIARKINNLSFAWTVTGPPSTQ
                     VLLRVRDSFRSSVFDVTSQKITIPGSVSGAPAPEPIRNNEITAAPNPSSGPVSFGLVL
                     ATAADVRLDVYDVAGRLVHSQVTGILQPGHHELAWNGSTDRGNRAPAGIYWARASWNG
                     FRAERRIVRLP"
     gene            1731..>2577
                     /locus_tag="VF720_01395"
     CDS             1731..>2577
                     /locus_tag="VF720_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878030.1"
                     /translation="MIPGRLLRAPGAALLALAVVASPRPASATAYSWKVANSSAWNNS
                     ANWTPAGIPGSGDDVTISISGTYTVNMNLNGGSATVRSLSITGSGAHSLQMIDATLTV
                     TNGPFVVGAGHEIIMYQSHLTGSAALQISGDIAASSTGGGADNTITNAVTTVAGSRIR
                     ILGGSGVSLSIQNAFTVNGELDLRGTSAPGQPNDAVFYLLSGTLTIGSGGKLISSVDT
                     GGGGRICHATINNQGTIQVDYPMTIDKLSADHVSTGTIDLNEGTLTWLQTGTTPSLTA
                     NGSITI"
BASE COUNT          440 a          888 c          823 g          426 t
ORIGIN      
        1 cgggtattcg tgtcgaacgg cgcggcggtc gacagcagcg acgccttctt ctcgatctgc
       61 cagagcctgg caagcggcac cctctatccc tcgaccggaa ccggaatcgg gatggctacc
      121 ggcgatttca acgaggacgg gatcaccgac gtggccgaag gggggtgcgg ggttcgcatc
      181 cacctgggcc tgggttcggg tggcgtcggc aacggcgcgt tcggaccggc ctcggtcgtt
      241 ccgctggtcg gctgcggtac ggccgtggcc accggcgatt tcaatgatga cggcatcacc
      301 gacctcgtgg ccatcgacgg cagcggcctc tccaccctgc ggggccaggg aagcggcggc
      361 acggggaacg gcagtttcgt ccggacggcc acctacccct tcccctccgg gccggaagga
      421 ctggccgtgg ccgacttcaa cgaggacggc atcgaagata tcgcggcggc gctcaggctc
      481 caggaccagg tgggcatcct gttcggcaac ggcgtgagcg gcgtcggaac cggcacgttc
      541 gcgccggtcg tgctggtgtc gaccggcgac ggtcccatgg acgtggcggt ccaggacttc
      601 aacgaggatg ggatctggga catcgccacc tcgaatgccg ttgccgccaa tgtctcgatc
      661 ctgatcgggc tcggtgcggg gagcatcggg aacgggacgt tcgccgcccc ggtccactac
      721 gccgggatag gcggcagtcg cttcctggct ctcgacatga gcaatgatgg catcgtcgac
      781 ctggtcggcg gctcggtgac cctgctcaag ggaaacggat ccggcggcgt cggaaacggg
      841 acgtttgccg ccgcggtttc gtccgtcggc ctctacccga ttcttggcat catggacatc
      901 gatcacaata cgctgcccga cctcgtcggc ctcggcgccg gtcgcctcga gctgatgcgc
      961 aacacttcct cgaccggaac tttcgaatcg gccggatggc tcccgtcgat gggcgccaac
     1021 ccgcagttcg ccatcgccgg cgactttctc gaggatcaga tcatcgacct ggccggcatc
     1081 tcgcacagtg agccggcgct ggcggtggcc ctcgcgggag gctgcgtcag tgaagcggtc
     1141 acgatcacgc tcgcggagcc gtcccccacc acctggaacg tcgggacgaa ccagacaatc
     1201 acctggagca agagcttcac gaatttcctc aacatccatg gctgggatgt cgaggtttcg
     1261 cgcgacaacg gcgtcaactg ggagacgatt gcccggaaga tcaacaacct gagtttcgcg
     1321 tggaccgtca ccggcccccc gagcacccag gtgctcctcc gggtccgcga cagcttccgc
     1381 tccagcgtgt tcgacgtgac cagccagaag atcaccatcc ccgggagcgt ctccggcgca
     1441 ccggcgcccg agccgatcag gaacaacgag atcaccgcgg cgcccaatcc ctcgtcgggt
     1501 cccgtgagct tcggtctcgt gctggcgacc gccgccgatg tccgcttgga tgtgtacgac
     1561 gtggccgggc gcctcgtgca ttcgcaagtc accggcatcc tgcagccggg ccaccacgaa
     1621 cttgcctgga atggctcgac cgatcgtggt aaccgggcac ccgccggaat ctactgggca
     1681 cgcgcctcct ggaacggttt ccgcgcggag agacgcatcg tgcgccttcc atgattccgg
     1741 gacgcctcct gcgtgcgcct ggtgccgcgc tcctggccct ggcggtcgtg gcatcacccc
     1801 ggcccgcctc ggccaccgcc tattcctgga aggtggcgaa cagctcggcg tggaacaatt
     1861 cggcgaactg gaccccggcc ggaattcccg ggtcgggcga cgacgtcacg atttccattt
     1921 ccgggacata caccgtgaac atgaacctga acggcggctc ggccacggtg cgctccctct
     1981 ccatcaccgg ctcgggcgcc cactcgctcc agatgatcga tgccacgctg acggtgacca
     2041 acggcccgtt cgtcgtgggc gccggccatg aaatcatcat gtaccaatcc caccttaccg
     2101 gctccgcggc gctccagatt tccggcgaca tcgcggcctc ttcgaccggg ggcggcgccg
     2161 acaacacgat cacgaacgcc gtcacgaccg tggccggatc gaggatccgg atcctggggg
     2221 ggtcgggtgt ctcgctgtcg atccagaacg ccttcacggt gaacggggag ctcgacctgc
     2281 gcggcacgag cgcccccggt cagccgaacg atgcggtctt ctacctcctg tcgggcacgc
     2341 tgaccatcgg atccggaggg aagctcattt cttccgtgga caccggtggc ggcggccgga
     2401 tctgccacgc cacgatcaac aaccagggaa cgatccaggt cgattacccg atgacgatcg
     2461 acaagctgtc ggccgaccat gtgagcaccg gcaccatcga cctgaacgaa ggaacgctca
     2521 cctggctcca gaccggaacc acgcccagcc tcaccgcaaa cggatcgatc accatcg
//
LOCUS       DASVOD010000071         3385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_265543, whole genome shotgun sequence.
ACCESSION   DASVOD010000071 DASVOD010000000
VERSION     DASVOD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265543"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..670
                     /gene="fsa"
                     /locus_tag="VF720_01400"
     CDS             26..670
                     /gene="fsa"
                     /locus_tag="VF720_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX7878031.1"
                     /translation="MKFFVDTANIAEIKELAELGIIDGVTTNPSLVAKEKGSYDDLLK
                     EICELVDGPISAEVTATDLDGMLKEGEHYASLHDNIVVKLPTIPEGLKACRRLVADDI
                     RVNMTLIFSPLQALLCARAGATFVSPFIGRLDDISQEGMGVIEQTVQIFDNYNFPTEV
                     LVASVRHPVHILQAAQIGADVCTVPHKILMQMLKHPLTDSGLEKFLADWKKSGR"
     gene            703..1893
                     /locus_tag="VF720_01405"
     CDS             703..1893
                     /locus_tag="VF720_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7878032.1"
                     /translation="MIPRPVRLRALALVLLGAGLVLAATNLWKSSPSGAPKAAPTRMA
                     GAPDPIGEGRRNAIVTAAEVAGKGVVSITAVESRTYTVSPFPRGFDELFSQYLGSVPG
                     QTYREDVPKFGSGFIFDHDGHILTNAHVVAGAEEITVTLADGRQITGKVLGGDLAYDL
                     AVIKIEGKDLPAVTVGNSDDLLVGEWAIAIGNPFGHLLGDSHPTVTVGVISATNRDIN
                     VNVAEGGIYKNMIQTDAAINRGNSGGALVNARGEVVGVNTFIFTEGGGSLGIGFAIPI
                     NTASEVAREIIQHGRVREVWIGISVVPLSGYLAQRLGTQDGRGCVVSQLERGSPADRA
                     GLEVGDIIRAIGNTPVADAPSARRQIFGAKAGDTLVMRVERAGRVKDFEVRLENMPDR
                     PPGR"
     gene            1890..3218
                     /gene="purB"
                     /locus_tag="VF720_01410"
     CDS             1890..3218
                     /gene="purB"
                     /locus_tag="VF720_01410"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEX7878033.1"
                     /translation="MIERYSRPEMARLFATPARFATWLDVEAAVSRALERRRLVPAGT
                     ADAIERAAPIDPARVDELEATTNHDVIAFLTAVTEKIGAPGRWLHFGMTSSDLVDTAQ
                     GLTLRRASMVLRSGLVDLSRVVRDRALEFRRQPCVGRTHGVIAEPTTFGLKLLGWYAE
                     LERQRARLDDATLGVAVGKISGAVGTFSHLSPEIEEEVCHRLGLTPEPVSTQVTARDR
                     HAALLTTLAGLASSLERMATEVRNLQRSEILEAEEHFAAGQKGSSAMPHKRNPITAER
                     VAGLSRLVRGYAVAALENVALWHERDITHSSVERVILADAFLVVDYQVSLMTRIVQGL
                     VVYPARMAANLDRAGGIVFSQRILLALTESGLPREEAYRLVQRAAHNAWQNNGSFRAL
                     AEADPEIVSRLDPGQLDDCFDIDHHLRHVDDIFRRVLGDDLLPGSFDDDA"
     gene            3211..>3385
                     /locus_tag="VF720_01415"
     CDS             3211..>3385
                     /locus_tag="VF720_01415"
                     /inference="COORDINATES: protein motif:HMM:NF037180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878034.1"
                     /translation="MPESLTDTDLAARLADARVRLTVEEARRVAGLLGREPTRVELVL
                     FDTMWSEHCSYKSS"
BASE COUNT          559 a         1198 c         1126 g          502 t
ORIGIN      
        1 gaatgagcgt caggaggaac attccatgaa attcttcgtc gacacggcca atatcgcgga
       61 aatcaaggag ctggccgagc tcggcatcat cgacggcgtc accacgaacc cgtcgctcgt
      121 ggccaaggaa aagggtagct acgacgacct gctcaaggag atctgcgagc tggtcgatgg
      181 ccccatctcc gccgaagtga ccgccaccga cctggacggc atgctcaagg aaggcgagca
      241 ctacgcctcg ctccacgaca acatcgtcgt caagctgccg acgatccccg aggggctcaa
      301 ggcgtgccgc cggctggtgg ccgacgacat ccgcgtgaac atgacgctga tcttctcgcc
      361 gctccaggcg ctcctctgcg cgcgggccgg cgccacgttc gtctccccgt tcatcgggcg
      421 cctcgacgac atctcccagg aggggatggg ggtcatcgag cagacggtgc agatcttcga
      481 caactacaac ttcccgaccg aggtgctggt ggccagcgtg aggcacccgg tgcacatcct
      541 gcaggccgcc cagatcgggg ccgacgtctg caccgtgccg cacaagatcc tcatgcagat
      601 gctcaagcac ccgctgaccg acagcggcct ggaaaaattc ctggccgatt ggaagaaatc
      661 cggacggtga gcgccagcct gccgcgaacc gatggccgcc cgatgatccc ccgccccgtt
      721 cgcctgcgcg cgctcgccct ggtgctgctc ggggccgggc tggtgctggc cgccaccaat
      781 ctctggaagt cgtctccgtc cggcgcgccg aaagcggccc cgacgcggat ggccggcgca
      841 ccggatccga tcggcgaagg acgtcgtaac gccatcgtca ctgcggccga agtggccggc
      901 aagggggtgg tgtcgatcac ggcggtcgag agccgcacct ataccgtctc cccgtttccg
      961 cgcggcttcg atgagctctt ttcccagtac ctggggagcg tgcccggcca gacctaccgg
     1021 gaagacgtcc ccaagttcgg cagcgggttc atcttcgacc acgacggcca catcctgacc
     1081 aacgcgcacg tggtggccgg cgccgaggag atcaccgtca cgctggccga cggtcggcag
     1141 ataaccggca aggtgctcgg cggcgacctg gcctacgacc tggccgtcat caagatcgag
     1201 gggaaggacc ttccggccgt gaccgtcggc aactcggacg acctcctggt cggcgagtgg
     1261 gccatcgcca tcggcaatcc gttcggccac ctcctggggg acagccaccc caccgtcacc
     1321 gtcggcgtga tcagcgccac caaccgcgac atcaacgtga acgtggccga ggggggcatc
     1381 tacaagaaca tgatccagac cgacgcggcc atcaaccgag ggaactcggg cggggcgctg
     1441 gtcaacgcgc gcggcgaggt ggtcggcgtc aacacgttca tcttcaccga ggggggtggc
     1501 tcgctcggca tcggcttcgc gattccgatc aacaccgcct ccgaagtggc cagggaaatc
     1561 atccagcacg ggcgcgtgcg cgaggtgtgg atcggcatca gcgtcgtccc cctctcggga
     1621 tatctggccc agcggctcgg cacgcaggat gggcgcggct gcgtcgtctc gcagctcgag
     1681 cggggaagcc cggccgaccg ggccggcctc gaggtggggg acatcatccg cgccatcggc
     1741 aacacgccgg tggccgatgc gccctcggcc cgccgccaga tcttcggcgc caaggcgggc
     1801 gataccctgg tcatgcgcgt ggagcgggcg ggccgggtga aggacttcga agtgcggctc
     1861 gagaacatgc cggaccgccc tcccggccga tgatcgagcg ctactcccgg ccggagatgg
     1921 cgcggctctt cgccacgccg gcccgtttcg ccacctggct cgacgtggaa gccgcggtga
     1981 gccgcgcgct cgagcggcgg cgcctcgtgc cggccggcac cgccgacgcc atcgaaaggg
     2041 cggcgcccat cgatcccgcg cgggtggatg agctggaggc caccaccaat cacgacgtca
     2101 tcgccttcct gacggccgtc accgagaaga tcggggcgcc ggggcgctgg cttcacttcg
     2161 gcatgacctc gagcgacctg gtcgacaccg cccagggact cacgctccgc cgggcctcga
     2221 tggtgctgcg cagcggcctg gtcgacctct cccgggtggt gcgcgaccgc gccctcgagt
     2281 tccgccggca accctgcgtg gggcggacgc acggcgtcat cgccgagccc accacgttcg
     2341 gcctcaagct cctcggctgg tacgccgagc tcgaacggca gcgcgcacgg ctcgacgacg
     2401 ccacgctcgg cgtggccgtc ggcaagatct ccggcgctgt gggcacgttc agccacctct
     2461 cgccggaaat tgaagaagag gtgtgccacc gcctcggcct cacgccggaa ccggtctcga
     2521 cgcaggtcac ggcgcgcgac cgccatgccg cgctcctcac cacgctcgcg ggcctcgcct
     2581 cctcgctcga gcggatggcc accgaggttc ggaatctgca aaggagcgag atcctcgagg
     2641 ccgaggagca ttttgcggcc ggccagaaag ggtcgagcgc catgccgcac aaacggaacc
     2701 cgatcacggc cgaacgggtc gcgggactct cgcggttggt gcgaggctat gccgtggccg
     2761 cgctcgagaa cgtggcgctc tggcacgagc gtgacatcac ccactcgtcc gtcgagcggg
     2821 tcattctggc cgatgcgttc ctggtggtcg actaccaggt gtcgctgatg acccgcatcg
     2881 tgcagggact ggtggtctac ccggcccgga tggccgccaa cctcgaccgc gccggcggca
     2941 tcgtcttctc gcagcggatc ctcctcgcgc tcactgaatc gggcctccca cgcgaggagg
     3001 cctaccggct ggtgcaacgg gccgcgcaca acgcctggca gaacaacggt tcgttccgcg
     3061 cgctggccga ggccgatccg gagatcgtga gccgcctgga cccggggcag ctcgacgact
     3121 gcttcgacat cgaccaccat ctgcggcatg tcgacgacat cttccgccgc gtgctcgggg
     3181 atgacctcct ccccggcagc ttcgacgacg atgcctgagt cgctcaccga taccgacctg
     3241 gccgcccggc tggccgacgc cagggtccgc ctgacggtgg aggaggcccg ccgcgtggcc
     3301 ggtctcctcg gtcgcgagcc gacgcgcgtc gagctggtcc tcttcgacac gatgtggagc
     3361 gagcactgct cgtacaagtc gagcc
//
LOCUS       DASVOD010000072         8535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_787346, whole genome shotgun sequence.
ACCESSION   DASVOD010000072 DASVOD010000000
VERSION     DASVOD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787346"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF720_01420"
     CDS             <1..451
                     /locus_tag="VF720_01420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7878035.1"
                     /translation="LSDDERVAVANSVGADLFISLHCDAHPSPAVSGPRAVVARARTG
                     PRPGMPGDLAELGFTAWGEGQRDQLPRSYRLADRLVTELAAEVDQPSRGVEEWPLPLL
                     DAATMPAAYLEVATLTAPGSERRLREDRDRMVTAIVRAVDRFRRENP"
     gene            448..1005
                     /locus_tag="VF720_01425"
     CDS             448..1005
                     /locus_tag="VF720_01425"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX7878036.1"
                     /translation="MRAPRRVLVGAGIALALLVIVLGVRALFQRPVGGGAGVVADDGL
                     GVGMQAVTLYFADPEGADLATERRDLVVGTQTSMLVSAALSALAEGPLDRLARTVPEG
                     TRIRHVFVDNDGAVYADFTSELARGMAGAGLSQEILLLRSLARTLDVNFRNLRTLVIL
                     VDGKAVPSLGGRIATDRPLVLSEWR"
     gene            1002..1955
                     /gene="murI"
                     /locus_tag="VF720_01430"
     CDS             1002..1955
                     /gene="murI"
                     /locus_tag="VF720_01430"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX7878037.1"
                     /translation="MTGGEAMSRGESGSVPTPGRPVAVFDSGIGGLTLVKALLERAPE
                     TSIVYFGDTARLPYGSKSDATIERQSLECLRFLLGFDPSLVVVACNTASSIALPALVR
                     SIDRPVVGVIDAAVTASLNATRSRKIGIIGTAATIRSGGYERRLRERASDLTLVNQAC
                     PLFVPLVEEGWIDHDVTRQVAREYLAPVLAAGIDTLILGCTHYPLLKPVLAEVAGPGV
                     TLIDTGAETAAEVASGLHARGGGAQGSVSAPGSVSVSTPGAAARGATPGTLRFFVSDV
                     PSRFEINGARFLGRPLGPVTLVEQSDVPWYARRSDIPKGKS"
     gene            1952..2689
                     /gene="rph"
                     /locus_tag="VF720_01435"
     CDS             1952..2689
                     /gene="rph"
                     /locus_tag="VF720_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018230665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX7878038.1"
                     /translation="MKRHDDRRPDQLRPFKLATGIFRHAEGSARVESGRTIVLCAATI
                     DDRVPGWLRGSGKGWVTAEYGMLPRCSHSRIAREAATGKQGGRTLEIQRLIGRSLRSV
                     TDLRSFGERSITLDCDVFEADGGTRTAAINGASVSLHMAFTRLVEQGLIPFHPMRDLV
                     AAVSVGIVAGTPCLDLDYEEDSAAGVDMNIVMTGSGQFVEVQGTAESTPFSNKELAAL
                     LRLGRSGIRRILLAQKKALGLPVVRRS"
     gene            2824..3444
                     /gene="rdgB"
                     /locus_tag="VF720_01440"
     CDS             2824..3444
                     /gene="rdgB"
                     /locus_tag="VF720_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEX7878039.1"
                     /translation="MRTPRFPRLVVATRNMGKLVELADGLAGFAPPIVLSPLSDWSGA
                     REVDETGDTCRENARLKAVSALEATGLPSLADDTALEVDALGGAPGVHSARYAGPDGD
                     ARANMTKLLEALEGVPPARRTARFRCVLALAEPGGDVSFTEGVVEGHILAERRGSAGF
                     GYDPLFRVDGLDRTMAELTTAEKSLVSHRGRALAALRTLLQRAEAG"
     gene            complement(3763..4674)
                     /locus_tag="VF720_01445"
     CDS             complement(3763..4674)
                     /locus_tag="VF720_01445"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7878040.1"
                     /translation="MTPFPNHWIVILAGGSGTRLRGWAGPDGAAHVPKCYRNLDGDGP
                     LIDGAWNRARRLAPAGRTVIVVAQDHEAWWLPLRARWPGAVWMVQRHDRGTGGAILSA
                     ALLVLGRDADAVMTILPADHAVRDDKAFAAALKGLRREAARQAEALILLGAPAGAGTD
                     EDGYGWIVPEPANHPTASNEPALRKVARFVPRPAPGAGDRLRADGALVDTFLVTGTAR
                     TLLKLFKLTRPTLVDGALENLLSSGLTPAALIRSQATLPHLDFGADVLASAAVAPHLL
                     VARLPVCGWADLGTPERVEAWRGQVPQ"
     gene            complement(4671..5753)
                     /locus_tag="VF720_01450"
     CDS             complement(4671..5753)
                     /locus_tag="VF720_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878041.1"
                     /translation="MTSRRSLPIRDLEAGYYLDLFLVAAVTAVLGIRFTLQLTGYPQV
                     AMGQLHIAHMLWGGLFMLAAIVLLLSYMGRGGDEAAALIGGLGFGTFIDEVGKFITRD
                     NDYFYQPSVAIIYVTFIVTWLAVRSIHRERTASPVEYLANAIKLLRKSASGALTAEER
                     QKTLEYLGKSDPSHPVVTPLKALVEALPASPDPLPGVLVRFRDVLSRGYRSLACRPMF
                     EVAITGFFIAQLLVKLASAALFIGIERLSPEVVARLPFLQPELLDDRTRAEWGQLGSS
                     LLSGLFVALGVIRIRRDRLGAFRMFQRSILVSIFLTQVFMFYQRRWDALLALAVNLLV
                     FLALRFMIERERAAREAYASGERGVP"
     gene            complement(6351..8291)
                     /locus_tag="VF720_01455"
     CDS             complement(6351..8291)
                     /locus_tag="VF720_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878042.1"
                     /translation="MFPLRNLPFVALLGLAFLAGVPSFAEAAPGADFRVLQDTTAVPD
                     GNAVLGVAPPWLITPKVLLAGDSWAQYMWDDGAHNDIFDRFGFEDRRAVSLSLGSDPG
                     AGYTGPEYAISGSEARQWSDTANYPWIANMVAALEANPTIEVVVLSIGGNDVLAGKPG
                     GGWYKQMDLDTPGAEAAFFDRLEDDTFAVIDAALAVRPSIRVMIASYEYPNFNVGFWC
                     FAYACPKRRDLSRDPNNALITDIELNDMMKRIEQRRIAWVASNPRLLFDHGIGLMHFY
                     HGDGQSAPRTLPYPGQMAPSYLPFPGGNPLRPTLRADFRRPNGIDADPIHLNAAGYQD
                     KVANETTTLFLPWFRGLSTGTFIAAGGVDDGWTDGTTAGTSGIRVGDTGTAPVAGLLS
                     FDTSSIPDGATITAASLFLTRNSLTGTNPFTSGAMGVPRLDMKRGTFGAAAVEVGDGT
                     APADGNDIGVAVGSVKGNGYALRIDLLPAGYTSVNDSGLTQFRLSFPAVSSGTTADYV
                     TFRDGESPVPPVTEPSTLAEFMATSAPFLDVSWSVPSGVADAARPGGLDFRGASPNPF
                     RAGTTLRFALSSAGPARLTIFDVTGRRVARLVDGDLPAGEQALSWDGRNESGAMTAPG
                     FYLARLESAGETRSVRIVRTGE"
BASE COUNT         1384 a         2846 c         3011 g         1294 t
ORIGIN      
        1 cctctcggac gacgagaggg tggccgtcgc gaactcggtc ggcgccgatc tcttcatcag
       61 tcttcactgc gatgcgcacc cttcgccggc ggtgtcgggc ccgcgggccg tggtggcccg
      121 cgcccggacc gggccgaggc ccggcatgcc gggagatctg gccgaactcg ggttcaccgc
      181 gtggggcgaa gggcagcgtg atcagctccc gcgttcgtac cggctggccg accggctcgt
      241 caccgagctg gccgccgagg tcgaccagcc gtcgcgcggt gtcgaggagt ggccgctccc
      301 gctgctggat gccgccacga tgccggccgc ctatctcgag gtggccacgc tcaccgcgcc
      361 gggaagcgaa cgccggctgc gtgaagaccg ggaccgcatg gtcaccgcca tcgtgcgcgc
      421 ggtcgatcga ttccgccggg aaaatccgtg agggcgccgc ggcgggtcct tgtcggcgcc
      481 ggaatcgcgc tggccctgct cgtcatcgtg ctgggcgtgc gcgccctctt ccagcgcccg
      541 gtcggtggtg gcgccggcgt ggtggccgac gacggccttg gcgtgggcat gcaggcggtc
      601 acgctctact tcgccgatcc ggagggggcc gacctggcga ccgagcgccg cgacctcgtg
      661 gtaggcacgc agacctcgat gctggtgtcg gcggcgcttt ccgcgctggc cgaggggccg
      721 ctcgaccgcc tggcgcgcac cgtccccgaa ggaacgcgga tccgccatgt cttcgtggac
      781 aacgacggcg ccgtctacgc cgatttcacc agcgagctgg cacgcggcat ggccggggcc
      841 ggcctctcgc aggaaatcct cctcctgcgt tccctcgcgc ggacgctcga cgtcaacttc
      901 cgcaacctcc ggacgctggt catcctggtg gacggaaagg cggtgccgtc gctcggcggg
      961 cgcatcgcca cggatcgccc gctggtgctg tcggagtggc gatgacaggc ggtgaggcga
     1021 tgtcgcgcgg tgagtcgggg agcgtgccga cgccgggccg gccggtggct gttttcgatt
     1081 caggtatcgg cggcctcacg ctggtcaagg cgcttctcga gcgggccccg gagacgtcga
     1141 tcgtctactt cggcgacacg gcgcgcctcc cctacggttc caagtccgac gccaccatcg
     1201 agcgccagtc gctcgaatgc ctccggtttc tgttgggatt cgatccgagc ctcgtcgtgg
     1261 tggcctgcaa taccgccagc tccatcgccc tcccggccct cgtccgctcg atcgatcggc
     1321 cggtggtcgg tgtcatcgac gctgccgtca cggccagcct caacgccacg cgcagccgga
     1381 agatcggcat catcgggacg gccgccacga tccgctccgg cgggtacgag cggcgccttc
     1441 gcgaacgggc gtccgatctc acactcgtga accaggcctg ccccctcttc gtgcccctgg
     1501 tggaagaggg atggatcgac cacgacgtga cgaggcaggt ggcccgcgaa tacctggcgc
     1561 ccgtgctcgc ggcgggaatc gacacgctga tcctgggatg cacgcactac ccgttgctga
     1621 agccggtcct ggccgaagtg gccggcccgg gcgtcacgct gatcgacacg ggggccgaga
     1681 cggcggccga ggtggcatcg gggcttcatg cccggggcgg cggcgcacaa ggctcagtgt
     1741 cggcgccggg ttccgtgtcc gtttccacgc ccggcgccgc cgcgcgcggc gccacccccg
     1801 gcacgcttcg cttcttcgtc agcgacgtgc cgagtcgctt cgagatcaac ggcgcccggt
     1861 tcctcgggcg accgctcggc cccgtcacgc tggtcgaaca gagcgatgtc ccctggtacg
     1921 cgcgccggtc cgacatcccg aaaggaaaat catgaagcgc cacgacgatc ggcggccgga
     1981 ccaactccgg cccttcaagc ttgccaccgg gatcttccgg catgccgagg gttcggcgcg
     2041 ggtggaatcg gggcgcacga tcgtgctgtg cgcggccacc attgatgacc gcgttcccgg
     2101 ctggctgcgc ggcagcggca aggggtgggt caccgcggag tacgggatgc tgccgcgctg
     2161 ctcccattcc cgcatcgccc gggaggcggc tacggggaag cagggggggc gcacgctgga
     2221 gatccagcgc ctcatcggcc gctccctccg ctcggtcacg gatctgaggt cgttcggtga
     2281 gcgctcgatc acgctcgact gcgacgtctt cgaggcggac ggtggcaccc gcacggccgc
     2341 catcaacggc gccagcgtgt cgctccacat ggcgttcacc cgattggtcg aacagggact
     2401 catcccgttc caccccatgc gcgacctcgt ggcggccgtc tcggtcggga tcgtggccgg
     2461 caccccgtgc ctcgacctcg actacgagga agattcggcc gccggcgtgg acatgaacat
     2521 cgtgatgacc ggcagcggcc agttcgtcga ggtgcagggg actgccgaat cgacgccttt
     2581 ctccaacaag gaactggcgg ccctcctgag gctcggccgc tccggcatcc ggcgcatcct
     2641 gcttgcgcag aagaaggccc tgggattgcc ggtagtccgg cggtcgtgag cgcattacga
     2701 gcatgaggac ctctcgcacg tcaggccacg cgcacgtgag ggccccgcgc ttcgcgcgcc
     2761 tcgtcgtggg cgcgcgcacg tgagaacccc gcgtttcccg cgcctggtcg tgggcgcgcg
     2821 cacgtgagaa ccccgcgttt cccgcgcctg gtcgtggcca cgcgcaatat gggaaagctg
     2881 gtggaactgg ccgacgggct ggccggattc gccccgccga tcgtcctttc gccgctgtcc
     2941 gactggtcgg gtgctcggga ggtcgacgag acgggtgata cctgtcgcga gaacgcccgg
     3001 ctgaaggcgg tttcggcgct cgaggccacc ggccttccct cgctggccga tgacacggcg
     3061 ctcgaggtcg atgcgctggg tggtgcgcca ggtgtgcaca gcgcgcgtta cgcggggccc
     3121 gacggggatg ctcgcgccaa catgaccaag ctgctcgaag cgcttgaggg cgtgccgccc
     3181 gcgcggcgca ccgcgcggtt ccgatgcgta ctggcgctcg ccgagccagg tggtgacgtg
     3241 agtttcacag agggtgtcgt cgaagggcat atccttgccg agcgccgcgg ctcggccggc
     3301 ttcggttatg acccgctctt ccgcgtggac ggcctcgaca ggacgatggc cgagctgacc
     3361 accgccgaaa agtcgctcgt gagccaccgc ggtcgggcgc tggccgctct gcggacgctg
     3421 ctgcagcggg ctgaagcggg ctgaggcgga ctgaagcagg ctgaagcggg cgacaggtgg
     3481 gatgtcgtgc ccgtcatccc gcggaggtaa gaagcaatca gtgttggtca ggcccctggc
     3541 ttcggcgttg cgatgctctg ggagaacctg ttccggaaca ggtaacgcgg agtggcccct
     3601 ggctccagcg ttgggattct ctgagcgaac ctgttccgga acaggtaacc gggagtggct
     3661 cctggctccg gcgttgcgac tctctaggag aacctgttcc ggaacaggta aggcgacgat
     3721 tcaccgcagc gacactgtta tggaatgagt atcccgattc ggtcactgcg gcacttgacc
     3781 gcgccacgcc tccactcgct ccggcgttcc gagatcggcc cagccgcaaa cgggcaagcg
     3841 cgccaccagg aggtgggggg caacggccgc agaggcgagg acgtcggcgc cgaagtcgag
     3901 atgaggaaga gtggcctgtg atcgtatcag ggcggccgga gtcagaccgg acgacaggag
     3961 attctcgagc gcaccgtcca cgagcgtggg acgagtgagt ttgaaaagct tgagcagggt
     4021 gcgcgcggtt cctgtcacga ggaaggtgtc gacgagcgcg ccatcggcgc ggagccggtc
     4081 gccggcgcca ggtgccgggc ggggcacgaa gcgagccacc ttccggagcg cgggctcgtt
     4141 cgaggcagtc gggtggttcg ccggttccgg cacgatccac ccgtagccgt cctcgtccgt
     4201 acccgctccg gccggcgccc caagcagtat cagcgcctcc gcttggcgag ccgcctctcg
     4261 ccggagcccc ttcaaggcgg cggcaaatgc cttgtcgtcc cgaaccgcat ggtcggccgg
     4321 caggatggtc atgacggcgt cggcatcgcg gcccagcacc aggagcgcgg ccgagaggat
     4381 ggcgccgccg gtgccgcggt cgtgccgctg caccatccag accgcgccgg gccagcgcgc
     4441 gcgaaggggc aaccaccagg cttcgtgatc ctgcgcgacg acgatcaccg tgcggcccgc
     4501 cggtgccagc cgccgcgcgc ggttccacgc gccatcgatc agcggaccgt cgccgtcgag
     4561 gtttcggtag catttgggga catgggccgc gccgtcgggg ccggcccagc cacgcaggcg
     4621 ggtgccgctg ccgccggcca ggatcacgat ccagtggttt gggaatgggg tcatggcacg
     4681 cctcgctccc cggaagcata cgcctctcgg gccgcccgtt cccgctcgat catgaatcgg
     4741 agcgcgagga agaccagcag gttgacggcc agggccagga gggcgtccca tcgccgctgg
     4801 tagaacatga agacctgggt caggaagatc gacaccagga tggagcgctg gaacatccgg
     4861 aaggcaccga ggcggtcccg gcggatccgg atgacgccga gcgccacgaa gaggcccgaa
     4921 aggagggagg agccgagctg tccccattcg gcccgggtgc gatcgtcgag cagttccggc
     4981 tggaggaagg ggaggcgggc caccacttcc ggcgacagcc gctcgatgcc gatgaagagc
     5041 gcggccgacg ccagcttcac caggagctgg gcgatgaaga agccggtgat ggccacctcg
     5101 aacatcggcc ggcatgccag gctccggtag ccgcgcgaca ggacatcgcg gaagcggacg
     5161 agcacaccgg gaagcggatc gggcgatgcc ggcagcgcct ccaccagtgc cttgaggggc
     5221 gtcacgacgg ggtgtgaggg gtcgctcttc ccgaggtatt ccagtgtctt ttggcgctcc
     5281 tcggccgtga gagcgccgct ggcggacttg cggagcagct tgatggcgtt ggccaggtac
     5341 tcgacgggcg aggcggtccg ttcccggtga atcgaacgga cagccagcca ggtgacgatg
     5401 aaggtcacgt aaatgatcgc caccgacggc tggtaaaaat aatcgttgtc acgcgtgatg
     5461 aacttcccga cctcgtcgat gaacgtgccg aagccgagcc cgccgatcag cgcggccgcc
     5521 tcgtcgccgc cccttcccat gtacgacagg agcagaacga tggccgccag catgaagagc
     5581 cctccccaga gcatgtgggc gatgtggagc tgtcccatcg ccacctgggg atagccggtc
     5641 agctggagcg tgaagcggat gccgagcacc gccgtgacgg cggccacgag gaagagatcg
     5701 aggtagtagc cggcctcgag gtcgcgaatc gggagcgagc ggcgggatgt catggggagc
     5761 aatctatcac ccgcgggtga ttaggtactg ccaccgcgtc attccgactc gtcctgggaa
     5821 gaaatgacac gacccgggcg ctcgcgacca acccacccca aaccgtgcga gcggcgtccc
     5881 gcgggggtaa gtgcgtgata cgaaagccgt ttcagcgacg acaccacaga ggcggcaccg
     5941 acggcacgcg tcttgatata cgagtccatc acgaagcgag tgacgaagca ggacgaacaa
     6001 acgcaagcga agcgccgaac gaagcagacc gaacaaacga ttccgaggcg ctggcccgtg
     6061 ccgattcagc ctaaatgacg gctattgaag cacgcgacat ggttgtcggg cgggctggcc
     6121 ttcaggaact tgaacgattc gtccgacggt gccatggaga ttcggaaaga acgataacaa
     6181 cggtcgaagt cagccatcac aacggacgac attgaccgat cgggaaaacc ggacctcagg
     6241 gcaccgccgg acattgagac agtccacacg tccgacaggc gcgtggttta cagcggccgc
     6301 gggatgacgc gagtcatgaa tcccgcggcc gtcatacatt tcccccggcc ctattcgccc
     6361 gtccgcacga tcctcaccga ccgggtctct cccgccgatt ccagccgcgc caggtagaag
     6421 ccgggcgccg tcatcgcgcc gctctcgttc cttccgtccc aggagagcgc ctgctcgccg
     6481 gcggggagat cgccgtccac cagccgcgcc acccgccgcc ctgtcacgtc gaagatcgtg
     6541 aggcgggccg gaccggccga cgacaacgca aacctcagcg tcgtgcccgc ccggaacggg
     6601 ttgggagagg ccccgcggaa atcgagcccc cccggccgtg ccgcgtcggc cacgccggac
     6661 ggaacgctcc agctgacgtc gaggaacggg gcggaggtgg ccatgaactc ggccagcgtg
     6721 gaaggttcgg tgacgggggg caccggcgac tcgccgtcgc gaaacgtgac gtagtcggct
     6781 gtcgtgcccg atgaaacggc agggaaggag agccggaact gggtgagacc gctgtcgttg
     6841 accgacgtgt acccggcggg caggagatca atgcggagcg catagccgtt ccccttcacc
     6901 gagccgaccg ccacgccgat gtcgttcccg tcggccggcg ccgtgccatc gcccacttcc
     6961 accgccgccg cgccgaacgt gccacgcttc atgtcgagcc gcggcacgcc catcgcgccc
     7021 gaggtgaacg ggttcgtgcc ggtcagggag ttgcgggtga ggaagagtga ggcggcggtg
     7081 atcgtggcac cgtccgggat cgaagaggtg tcgaacgaca gcaggccggc caccggggcc
     7141 gtgccggtat cacccactcg gattcccgag gtgccggcgg tcgtgccgtc cgtccagccg
     7201 tcatccacgc cgcccgccgc gatgaacgtc ccggtggaaa ggccgcggaa ccaggggagg
     7261 aagagcgtcg tcgtctcgtt ggccaccttg tcctggtagc cggccgcgtt caggtggatc
     7321 gggtcggcgt caatgccgtt gggacggcgg aagtcggccc gcaacgtggg gcggagcggg
     7381 ttgccccccg ggaacggcag atagctcggc gccatctgcc ccgggtaggg gagcgtgcgc
     7441 ggggccgact ggccatctcc gtggtagaag tgcatgagcc cgatcccgtg gtcgaaaagg
     7501 aggcgcgggt tcgaggccac ccaggcgatc cgccgctgct cgatccgctt catcatgtcg
     7561 ttgagctcga tgtcggtgat cagggcgttg ttcggatcgc gcgagagatc gcggcgcttg
     7621 gggcacgcgt acgcgaagca ccagaacccc acgttgaagt tcggatactc gtagctcgcg
     7681 atcatcacgc ggatcgacgg ccgcaccgcc agcgccgcgt cgatcacggc gaaggtgtcg
     7741 tcctcgaggc ggtcgaagaa ggccgcctcg gcgccgggcg tgtcgagatc catctgcttg
     7801 taccagcccc cccccggctt tcccgccagg acgtcgttgc cgccgatcga tagcaccacc
     7861 acttcgatgg tggggttggc ctcgagcgcc gccaccatgt tggcgatcca gggatagttg
     7921 gccgtgtcgc tccactggcg cgcctcgctc cccgaaatgg cgtactcggg ccccgtgtat
     7981 cccgcgcccg ggtcgctgcc gagcgagagg ctcaccgccc gccggtcttc gaagccgaag
     8041 cgatcgaaga tgtcgttgtg cgccccgtcg tcccacatgt actgcgccca gctgtcgccg
     8101 gccagcagca ctttgggcgt gatcagccag ggaggcgcga caccgaggac ggcattgccg
     8161 tcgggaaccg ccgtggtgtc ctgcaggacg cggaagtccg cgccgggcgc ggcctcggca
     8221 aacgatggaa ccccggcaag gaaggccagt ccgaggagcg cgacgaatgg caagttgcga
     8281 agcgggaaca tggaatcgtc ctccggacgg ggtaggtgag cgacaaggat agccgacagg
     8341 cccggcgagg ggacaagagg atttccgctt cgcggaaagg agtggccgat ccgccaccct
     8401 tccataaact ccagtatggt ggctccacga gttcgtcggc ttgacaccga ccgcccgcgg
     8461 gtggtccggt accggccatg agggagatgc gtcggtcgcc ggccgctttc cggctcgtcc
     8521 tgcccctcct cgtcg
//
LOCUS       DASVOD010000073         2815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_369977, whole genome shotgun sequence.
ACCESSION   DASVOD010000073 DASVOD010000000
VERSION     DASVOD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369977"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VF720_01460"
     CDS             complement(<1..485)
                     /locus_tag="VF720_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008700602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7878043.1"
                     /translation="MTTPNRYDAIVVGAGHAGCEAAYALARMGHRTLLLTLNLDGVAQ
                     MSCNPAIGGLGKGQMVREIDALGGLMGRAIDATGIQFRMLNRSKGPAVRSPRAQAEKK
                     GYQEWVRQVIEATPNLELRQGQSVRLLVEKTSAGNTVRGVGMLTGREYEAPVVILTPG
                     T"
     gene            complement(496..1881)
                     /gene="mnmE"
                     /locus_tag="VF720_01465"
     CDS             complement(496..1881)
                     /gene="mnmE"
                     /locus_tag="VF720_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEX7878044.1"
                     /translation="MTSPPDVIVAPATPAGTGALAIVRLSGAGAEEVADRMFRGRRTL
                     SSTPSHFLTLGLAVDPRFDDEPIDEVMAVVMRAPKSPTGETVVELSSHGSPEITAALV
                     DVLVATGARPAAAGEFTRRAFLNGRIDLAQAEAISELVWARGRRARRNAMAQLRGGIS
                     ERLGVLRGRLLDELAPLEAFIDFGDDVPEAPGQDRLLGAITEVRAGLARLLGGRERAR
                     LMRCGATVALAGRPNVGKSSLLNAMAGEERALVHAEPGTTRDLVDADISIGGYAVRLV
                     DTAGIRNTTDPVEAAGVDRARRIMEEADVTLLVLEAPTAPFDDDRRIAETLEPARMMV
                     VRNKIDLGDDDDCRRFAETCAGGAPVVPVSARRGDGLDQLIGQISGRLRDSAGDEEGV
                     ILTAARHLDAMERADNGLARAALALSDGAWADMVAAELRESLSALGEITGEQVGPEIL
                     DRIFSRFCIGK"
     gene            complement(1878..>2815)
                     /locus_tag="VF720_01470"
     CDS             complement(1878..>2815)
                     /locus_tag="VF720_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YidC/Oxa1 family insertase periplasmic-domain
                     containing protein"
                     /protein_id="HEX7878045.1"
                     /translation="KERRKEVTGEIHWVAVRNKYFMAAIVPPRSSVTTAAATGDAATH
                     LAGAVLRYPVSGAGTFHQSMKLYLGPIDYWQLKKLGLGLEFAIDLGWKIILPVAQLLL
                     WALNAGYKLIPNYGVVIILLSALTRLLFYPMTQISMRSMRAMQEIQPEMERLRKKYEK
                     DPQRLNQEVFALYRKHNVNPVSGCLPLLLQMPVFFALYSVLANSVSLRQAPFVGWITD
                     LSAPDTLATIGGFAIHVLPVLMSLSMFWQQKLTPSDPRQAPVMLLMPFMMLFFFYKVP
                     SGLTLYWTVTNLFSVAQQWQMNRETKARAAAVVTT"
BASE COUNT          442 a          971 c          953 g          449 t
ORIGIN      
        1 aacgttcccg gggtcaggat gacgacgggc gcctcgtatt cgcggccggt cagcatgccc
       61 acgccccgga ccgtattccc ggccgacgtc ttctccacga ggaggcgtac cgactgcccc
      121 tgccggagct cgaggttcgg cgtggcctcg atcacctgcc gcacccactc ctggtatccc
      181 tttttctcgg cctgggcgcg cggcgaccgg acggccggcc ccttgctgcg gttgagcatg
      241 cggaactgga tcccggtggc atcgatcgcc cgtcccatga ggccgccgag tgcgtcgatt
      301 tcccgcacca tctgcccctt tccgaggcca ccgatcgccg ggttgcagct catctgggcc
      361 acgccgtcga ggttcagcgt gaggaggagc gtgcgatgac ccatccgggc cagcgcgtac
      421 gcggcttcgc accccgcgtg gcccgcaccc accacgatcg cgtcgtaccg attgggcgtc
      481 gtcatcgctg gctcctcatt taccgatgca gaagcggctg aagatccggt cgaggatctc
      541 cggaccgacc tgctcgccgg taatttcgcc gagcgcggag agcgactcgc gcagctccgc
      601 cgccaccatg tcggcccacg cgccgtccga gagggcgagg gcggcgcggg cgagcccgtt
      661 gtcggcccgc tccattgcgt cgagatgacg cgccgcggtc aggatcaccc cctcctcatc
      721 cccggcggag tcccggagcc ggccggagat ctgcccgatc agctggtcga gcccgtcgcc
      781 gcggcgcgcc gacaccggga cgaccggtgc ccctccggcg cacgtctcgg cgaaccgtcg
      841 gcagtcatcg tcgtcgccga gatcgatctt gttgcgcacg accatcatgc gggccggctc
      901 gagcgtttcg gcaatccgtc ggtcgtcgtc gaacggcgcc gtgggcgcct cgagcaccag
      961 gagcgtgacg tccgcctcct ccatgatgcg acgggcgcga tccaccccgg cggcttccac
     1021 cggatcagtg gtgttgcgga tgccggcggt atcgacgagc cgcaccgcgt agccgccgat
     1081 ggaaatgtcg gcatcgacca ggtcgcgcgt ggtgcccggc tcggcgtgca cgagggcccg
     1141 ttcttcgccg gccatggcat tgagcagcga cgatttgccg acgttcggcc ggcctgccag
     1201 ggccacggtg gcgccacaac gcatcaggcg cgcccgctcg cgccctccga gcagccgggc
     1261 caacccggcc cgcacctccg tgatggcacc gagcaggcga tcctgccccg gcgcctccgg
     1321 gacatcgtca ccgaagtcga tgaaggcttc gagcggcgcc agctcatcga gcaggcggcc
     1381 gcgaagaaca ccgagccgtt ccgaaatgcc gccgcgcaac tgggccatcg cgttgcgtcg
     1441 cgcgcgacga ccgcgggccc acacgagttc gctgatcgcc tcggcctggg ccaggtcgat
     1501 ccggccgttc aggaaggcgc ggcgggtgaa ctcgccggcg gcggccggtc gtgcaccagt
     1561 ggccaccagc acgtcaacca gtgccgcggt gatctccggg ctcccatgac tgctgagctc
     1621 gacgacggtt tcgccggtcg gactcttcgg ggcccgcatc acgactgcca tgacttcgtc
     1681 gatcggttcg tcgtcaaacc gcggatcgac cgcgaggccc agcgtgagga agtggctcgg
     1741 cgtggacgaa agcgtgcggc ggccgcggaa cattctgtcc gccacctcct ccgcaccggc
     1801 gcccgaaagg cgcacgattg ccagggcccc cgttccggcc ggcgtggccg gcgcgacgat
     1861 gacgtccggc ggggaggtca cgtggtgacg accgccgccg cccgcgcctt cgtctcgcga
     1921 ttcatctgcc actgctgcgc caccgagaag agattggtga cggtccagta gagcgtaagg
     1981 cccgagggaa ccttgtagaa gaagaagagc atcatgaacg gcatcagcag catgaccggc
     2041 gcctggcgcg gatcgctcgg cgtgagcttc tgctgccaga acatgctcag cgacatcagc
     2101 accggcagga catggatggc gaagccgccg atcgtggcca gcgtgtcggg cgcggaaaga
     2161 tcggtgatcc agccgacgaa cggcgcctgg cgcaggctga ccgaattggc cagcacgctg
     2221 tagagcgcaa agaagaccgg catctgcagg aggagcggca ggcatcccga gaccgggttc
     2281 acgttgtgct tgcggtagag ggcaaagact tcctggttca ggcgctgcgg gtccttctcg
     2341 tacttcttcc gcaggcgctc catttccggc tggatttcct gcatggccct catggagcgc
     2401 atgctgatct gcgtcattgg atagaagagg agccgggtaa gcgccgacag aaggatgatg
     2461 accacgccgt agttcggaat cagcttgtat ccggcgttga gggcccacag cagcaactgg
     2521 gccaccggga ggatgatctt ccagccgagg tcgatggcga actcgaggcc gaggcccagc
     2581 ttcttgagct gccagtagtc gatcgggccg aggtagagct tcatcgactg gtggaacgtg
     2641 cccgcgccgc tcaccgggta gcgcagcacg gcgccggcca ggtgcgtggc cgcgtcgccg
     2701 gtggcggcgg ccgtcgtcac cgacgacctg gggggcacga tggcggccat gaagtacttg
     2761 tttcggaccg ccacccagtg gatctcgccg gtcacttcct tgcggcgttc cttct
//
LOCUS       DASVOD010000074         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_944119, whole genome shotgun sequence.
ACCESSION   DASVOD010000074 DASVOD010000000
VERSION     DASVOD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944119"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2242)
                     /locus_tag="VF720_01475"
     CDS             complement(<1..>2242)
                     /locus_tag="VF720_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878046.1"
                     /translation="LATGATIGAGGYTVLGQEQGAPGGGFNAARSFQGQLDELRIWNV
                     VRSAADIAATRAARLDGAEENLALYWPMDEGSSTVVADDTDGGTYATLLSGVTWSGNT
                     APVSVCGLTDSEGNYVLPEIRYGTSTTFRVSPSFPNRQFEPAFKTITLNTDNPVQNEV
                     AFSDISSYTIQGFVRFSGNDVEGDAYNCPVGEVEILVDGVLRGTTGPDGKYSIPVDLG
                     THEITARLGNHVFSPASRSQFIGADTANLDFADLTRRTLSGIAAGGCNLPIGTVDYEI
                     RSESSCFVIEDDTDGSWSQLLPAQKYTVRVIDVTLPTGSPLDRADILEFFENVGPQTV
                     DLRNADATADFIYHAPLVIEIEGFTDPACATLTLPGNPVPLPAVPVVEQSESVPLIIR
                     VFEDYGSAGLCPVDSGLVTVFDEIIDEGDTPVPLELRNGEARYTTIGNTPNIFAGRRD
                     QQGNDRSYQKPISFVAEVEGQAPVTFTRWVVVTGQRPRVATFTSVSEEIPLLILRDPP
                     GDASSAFLETGTTICNEIHNLGLENLTTGINLRVKTGLKFDKGTPFFTTTTEARVETE
                     NTFRFGFEATQDDGISICATTLERISTSPSDQFVDNRGDVYMGVALNLIFAKTDVVEV
                     NQSGSTCAIQKSESVTMGGEGFETTYIYSEEHIRAVLIPQNEELAALTGDTYYQSAAD
                     NWRAHIALNDSLKAAATFSKNRSFSAGADYEFSASSDTTGSFAYSTKLFTNDEVAIGF
                     NFDESG"
BASE COUNT          341 a          716 c          777 g          408 t
ORIGIN      
        1 ctgcccgact catcgaagtt gaagccgatg gccacctcgt cgttcgtgaa cagcttggtg
       61 gagtaggcga agctgccggt cgtgtcgctg gacgccgaga attcgtagtc ggcgccggcc
      121 gagaacgacc ggttcttgga aaacgtggcc gccgccttga gcgaatcgtt cagggcgatg
      181 tgcgcccgcc agttgtcggc cgcgctctgg tagtaggtat cgccggtcag ggcggccagc
      241 tcctcgttct gcgggatcag gacggcgcgg atgtgctcct cgctatagat gtaagtcgtc
      301 tcgaagcctt cgccgcccat ggtgaccgac tcggacttct ggatggcgca ggtgctgccg
      361 gactggttca cctcgaccac gtcggtcttg gcgaagatca ggttgagggc cacccccatg
      421 tacacgtcgc cgcggttgtc cacgaactgg tcgctcggcg aggtggaaat cctctcgagc
      481 gtcgtcgcac agatcgagat gccgtcgtcc tgggtggctt cgaagccgaa gcggaaggtg
      541 ttctccgtct cgacgcgggc ctcggtggtg gtggtgaaga acggggttcc cttgtcgaac
      601 ttgaggcccg tcttgacccg caggttgatc ccggtcgtca ggttctccag cccgaggttg
      661 tggatctcgt tgcagatcgt ggtgccggtc tcgaggaagg ccgaggaggc gtcgcccgga
      721 ggatcgcgca ggatcaggag cgggatctcc tccgacaccg aggtgaacgt ggccacgcgc
      781 gggcgctgcc ccgtcaccac gacccagcgg gtgaaggtga ccggcgcctg gccctccact
      841 tcggccacga acgagatcgg cttctggtat gaccggtcgt tcccctgctg gtcccgacgg
      901 ccggcaaaga tgttcggcgt gtttccgatg gtcgtgtagc gggcctcgcc gttccgcagc
      961 tcgagcggaa ccggcgtgtc gccttcgtcg atgatttcgt cgaacaccgt gacaaggccg
     1021 ctgtccaccg ggcacaaccc cgcggagccg taatcctcga acacccggat gatcagcggt
     1081 acgctctcgc tctgctccac gaccggcacg gccggcagcg gaacggggtt tccgggcagg
     1141 gtcagggtgg cgcaggccgg atcggtgaat ccctcgatct cgatcacgag cggcgcgtgg
     1201 tagatgaagt ccgcggtcgc gtcggcgttc cggaggtcca cggtctgtgg ccccacgttc
     1261 tcgaaaaact cgaggatgtc ggcccggtcg agcggcgaac cggtcggcag cgtcacgtcg
     1321 atgacccgga cggtgtactt ctgggccggc aggagctggc tccagcttcc atccgtgtcg
     1381 tcctcgatca cgaagcagct gctttcgctg cggatctcgt agtccaccgt cccgatcggc
     1441 aggttgcagc cgccggcggc gatgcccgac agcgtacgcc gcgtcaggtc ggcgaagtcg
     1501 aggttggcgg tgtcggcgcc gatgaactgc gaccggctgg ccggcgagaa gacatgattg
     1561 cccagccggg ccgtgatctc gtgcgtgcca aggtccacgg ggatcgagta cttcccgtcg
     1621 ggtccggtcg tcccgcggag gacgccgtcc accaggatct cgacctcgcc gaccgggcag
     1681 ttgtaggcat ccccctccac gtcattccca ctgaagcgga cgaagccctg gatggtgtag
     1741 gaggagatgt cgctgaacgc cacctcgttc tgcaccgggt tgtccgtgtt gagcgtgatc
     1801 gtcttgaacg ccggctcgaa ctgccggttc gggaacgacg ggctcacccg gaacgtggtg
     1861 gaggtgccgt agcggatctc gggaaggacg tagtttccct ccgagtcggt gaggccgcac
     1921 acgctcaccg gggcggtatt gccgctccag gtgacaccgg agaggagcgt ggcgtaggtg
     1981 ccgccatcgg tgtcgtcggc caccacggtg ctggacccct cgtccatcgg ccagtacagc
     2041 gccaggttct cctcggcgcc gtcgagccgg gcggcacggg tggcggcgat gtcggctgcc
     2101 gaacgcacga cgttccagat ccgaagttcg tcgagctgcc cctggaagct ccgcgcggcg
     2161 ttgaagccgc cgcccggcgc gccctgctcc tggccgagga ccgtgtagcc gcccgccccg
     2221 atcgtcgcac cggtggccag cg
//
LOCUS       DASVOD010000075         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_370046, whole genome shotgun sequence.
ACCESSION   DASVOD010000075 DASVOD010000000
VERSION     DASVOD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370046"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF720_01480"
     CDS             complement(<1..559)
                     /locus_tag="VF720_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7878047.1"
                     /translation="MDITPAWIAGRPVIGGGGVLDINSPWSGTVVRRVSLGTGSDVDA
                     ALAAATSAFEITRRASAADRSGWLERMAAQLVKRRDELAAAISNEAGKPIAMARTEVD
                     RAVQTFKTGAEEARRIGGEVLPLDTAPAGLGRFGITRRYPRGVVSGIVPFNFPLNLAA
                     HKVAPALASGNTIVLKPPPQAPGATL"
     gene            complement(702..>3399)
                     /locus_tag="VF720_01485"
     CDS             complement(702..>3399)
                     /locus_tag="VF720_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878048.1"
                     /translation="VRTNLKNNIRETTLENNLESSTAPFLVDIPELVLDVPATITLSP
                     GDSRYYRLASPAGFDLSVTVNSDDADATNELYMAFDRTPTPGDFDFTGPATFTARPSI
                     LVPSSQAGDYYLLVRAASLSSGFTAETVTIRARALPFSIASVSPGTIGSPGEVTITLN
                     GAGFRPTTEISLAVAGGFEIPASRVRFINTTRLSARFQVPQGMLGPVLVVARNPGDNA
                     ETNLANALVIEPATAQAVVTTAVRQDGVRNNAPAYFTFRYRNVSNVDVAHFQARLILP
                     GTAHIVAVQTSPDFLRRSTRYPELFQPTADDLIELPNTLKGESIQAIDLETAHLGPDA
                     EVIVTLNVTNFPVTPFSILSLTELSDTPTWLGRQITKIESARQTLVANSSGVPAEVLS
                     LAADPVAFRNQALEADHVPGGLLDSEDVGNLPGTVESIALAGLGDPFAGAAPPTTEEG
                     VDCARPAKTPDCTLDGTPPACALPHCLACFQAPIPVTLRLGGGVTVSVQEPTCTPYTF
                     EQCIGADVVAPCDPNTLTGPPGFGTDHWVGITQPLLFTANFENLSGVATAPAQVVQIT
                     LPLHANLDLSSFRLGSFGFGSHVIEVPPNRSSYTVEPYFSDLNLRVRVTAGVDVNNRQ
                     AFWTFTSIDPATGQLPSNPQVGFLPVNDTFGRGQGFCNFTVKPFAGAGTGAPIAMQAS
                     VKFDTNVPVLTNTTGHKVDAGRPVSSLAGAPQAVNASTVRVNWNGSDGASGSGVATYD
                     LYMQQNGGTFQFVTGGITGNTATLALTPGKSYGFYTIALDNSGNPESAKNTADGTISL
                     GSGTGVPDASVPPAFQLAQNYPNPFRSATTIEFDLPVEAPARLRVYDVAGRLVATYLD
                     GRPMPAGRHTLNVEAGSFGNGVYFYHLEAGGKVFTRKMLVLR"
BASE COUNT          522 a         1057 c         1281 g          539 t
ORIGIN      
        1 cgagcgtggc gcccggggcc tgcggcggcg gcttcagcac gatggtgttg cccgaggcca
       61 gggcgggcgc caccttgtgc gcggccaggt tgagcggaaa gttgaagggc acgatgccgg
      121 acacgacgcc ccgcgggtaa cggcgggtga tgccgaagcg cccgagtccg gccggcgccg
      181 tgtcgagggg gagcacctcg ccgccgatgc gacgggcttc ctcggcgccg gtcttgaagg
      241 tctggacggc gcggtccact tccgtgcgcg ccatcgcgat cggcttgccg gcctcattcg
      301 agatggcagc ggccagttcg tcgcggcgtt tgacgagctg cgcggccatc ctctcgagcc
      361 agccgctccg gtcggcggcg gaggcgcggc gcgtgatttc gaaggcgctt gtcgccgcgg
      421 cgagcgcggc atcgacgtcg gaccccgtgc cgagcgacac gcggcgaacc acggtcccgc
      481 tccagggaga attgatgtcg agcacgccgc cgccgccgat gaccggtcgg ccggcgatcc
      541 aggccggagt gatgtccatg ccggaattct ataccggcat gaggcggccc gggcgacgag
      601 tcacgcccaa gggccggtcg gaggcatgac cggtccatcg acccatcgcc cgggcccctg
      661 ttgcgcgacc accaactcgt ggcgcggggc ctattttccc gctaacgcag cacgagcatc
      721 ttgcgggtga agaccttccc tcccgcctcc aggtggtaga agtagacgcc gttcccgaac
      781 gaaccggcct ccacgttgag cgtgtggcgc cccgccggca tcggccgtcc gtcgaggtag
      841 gtggccacga gtcgccccgc cacgtcatag acgcggagcc gcgcgggtgc ctcgaccggc
      901 aggtcgaact cgatggtcgt ggccgagcgg aacgggttgg gatagttctg ggccagctgg
      961 aacgcggggg gcaccgacgc gtcggggacg ccggttccgg agccgaggga aatggttcca
     1021 tccgcggtgt tcttggcgct ctccggattt cccgagttat cgagggcgat ggtgtagaag
     1081 ccgtacgact tgccgggcgt gagcgccagg gtggccgtgt ttccggtgat gcctccggtc
     1141 acgaactgga acgtccctcc gttctgttgc atgtagaggt cataggtggc cacgccgctt
     1201 cccgaggcgc cgtcgctgcc gttccagttg acccggaccg tgctggcatt cacggcctgt
     1261 ggcgcgccgg ccaggctact gacggggcgg ccggcatcga ccttgtggcc cgtggtgttg
     1321 gtgaggaccg gcacgttcgt gtcgaacttg accgaggcct gcatggcgat cggcgcgccg
     1381 gtgcccgccc cggcgaacgg cttgaccgtg aagttgcaga acccctggcc gcgcccgaac
     1441 gtgtcgttca ccggcaggaa gcccacctgg ggattggacg gcaactgccc ggtggccggg
     1501 tcgatggagg tgaatgtcca gaaagcctgg cgattgttca cgtccacgcc cgccgtcacc
     1561 cggacgcgca ggttcaagtc ggagaagtag ggctcgacgg tatacgacga acggttgggc
     1621 ggcacttcga tcacgtgcga accgaagccg aagcttccca gccggaacga cgagaggtcg
     1681 agattggcgt gcagcggaag cgttatctgc acgacctggg ccggcgcggt ggccacgccc
     1741 gagaggttct cgaagttggc ggtgaagagg agcggctgcg tgatccccac ccagtggtcc
     1801 gtgccgaagc cgggggggcc ggtaagggtg ttgggatcgc agggcgccac gacgtcggct
     1861 ccgatgcact gttcgaacgt gtacggagtg caggtgggct cctggaccga aaccgtcacg
     1921 ccgcccccca ggcggagcgt gaccgggatc ggcgcctgga agcaggccag gcagtgcggc
     1981 agcgcgcagg ccggcggggt gccgtccagc gtgcagtcgg gcgtcttggc gggtcgcgcg
     2041 caatccaccc cttcttcggt ggtcggcggc gccgcaccgg caaacggatc gccgagcccg
     2101 gccagggcga tcgactcgac ggtgccgggg agattgccga cgtcctcgct gtcgaggagc
     2161 cctcccggta catggtcggc ctcgagcgcc tggttgcgga acgccaccgg gtcggccgcc
     2221 aggctcagca cctcggcggg aacaccgctg gagttggcca ccagcgtctg gcgcgccgat
     2281 tcgatcttcg tgatctgccg gccaagccag gtgggcgtgt cggacagctc ggtgagcgac
     2341 aggatggaaa atggcgtgac cgggaagttg gtcacgttca acgtgacgat cacctcggca
     2401 tcggggccca ggtgggcggt ctcgagatcg atggcctgga tcgattcccc cttcagcgta
     2461 ttggggagct cgatcaggtc atcggcggtg ggctggaaca gctcgggata gcgcgtgctc
     2521 cggcggagaa agtcggggct cgtctggacg gccacgatgt gcgccgtgcc cggcaggatc
     2581 aggcgggcct ggaaatgggc cacgtccacg ttcgagacgt tgcggtaacg gaaggtgaag
     2641 taggccggcg cgttgttgcg cacgccgtcc tgccgcaccg cggtggtgac caccgcctgc
     2701 gccgtggccg gttcgatgac cagcgcgttg gcgaggtttg tctcggcatt gtcgccgggg
     2761 ttgcgggcca cgacgaggac cgggccgagc atcccctgcg gcacctggaa gcgtgccgaa
     2821 aggcgcgtgg tgttgatgaa acggacgcgc gatgccggaa tttcgaatcc gccggccacc
     2881 gccagggaga tttcggtggt cgggcggaat ccggcaccgt tgagcgtgat cgtgacctcg
     2941 ccgggcgacc cgatggtccc gggcgacacc gacgcgatgg agaaggggag cgcccgcgcc
     3001 cggatcgtca ccgtttcggc ggtgaagccc gaggagaggc tggcggcgcg gacgagcagg
     3061 tagtaatcgc ccgcctgcga ggacggcacc aggatggagg gcctggccgt gaaggtggcc
     3121 ggcccggtga agtcgaaatc gccgggggtc ggcgtgcgat cgaacgccat gtagagctcg
     3181 ttggtggcgt ccgcgtcgtc cgagttcacc gtcacgctca ggtcgaagcc ggccggcgag
     3241 gccaggcggt agtaacggga atcgccgggg gacagggtga tcgtggcggg cacgtcgagc
     3301 accagctcgg ggatgtcgac caggaacggc gcggtcgaac tctcgaggtt gttctcgagc
     3361 gtggtctcgc ggatgttgtt cttcaagttg gtgcggacc
//
LOCUS       DASVOD010000076         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_839699, whole genome shotgun sequence.
ACCESSION   DASVOD010000076 DASVOD010000000
VERSION     DASVOD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839699"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1006
                     /locus_tag="VF720_01490"
     CDS             119..1006
                     /locus_tag="VF720_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878049.1"
                     /translation="MSLLAFAAIALTATIAAADRGIHEGPVVGGANVPSNQEVLYRSY
                     GECNDAFTSGITIRTVGGGNITIPPQVAANAVWAEIFWVTLADAPDAGAVTVNGNAVA
                     PLANGPVTVSPCWPQDFAFSYRANVLPFIQGGVNQLRGFAESGVDNVSPSTEGVTMVV
                     VYRTNTADKEIVVLGGNDSAILNQIDLAIPVVSAPGFGAELTLVVADGQPASDVALWN
                     GLVISAPDAFQGLDPGPGIGYWDTQEYGVATGGGNVASVIGQVDCLNWVATVLGVKRG
                     GCVVPVEPSTWSKVKNRFN"
     gene            complement(1110..1301)
                     /locus_tag="VF720_01495"
     CDS             complement(1110..1301)
                     /locus_tag="VF720_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006618702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEX7878050.1"
                     /translation="MIVEATFECAYCGETNETTVDLSAGEVQEYIEDCRVCCRPNNLY
                     VTGRASSGEAWIESFTDDE"
     gene            complement(1349..>2494)
                     /locus_tag="VF720_01500"
     CDS             complement(1349..>2494)
                     /locus_tag="VF720_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX7878051.1"
                     /translation="RTMTWQSDSPVNFFNVVAARWAEKRGNGTVIYYHPGHDYNVAEM
                     SEALDAARKYFSEWFYPFPWQELKLSEFPGLSAYAQGFPTDITFSEQIGFLTKSDPRS
                     NLAFMVTAHEAAHQWWGNILMPGEGPSGDILSEGMAHFSTILLFDQVKGPEQRIEFLK
                     RIESRYGERRQVDSERPLVKIDGSKDGDQTVTYDKGGWVFWMLLNEMGRDSALAGNSA
                     FIHKYAHNRDHALLPDYVEVMRGFAPDTTAYDAFVDQWFHQVVVPEYKLAGATKKRVG
                     DTWEVTVEVTNAGTGVMPVEVAAITGERFPKRKGAPAPGSDAAPAAHAAEKESKDPYL
                     EVRTTVRPAPGEKVNVQLTCPFEPKSILVDPDARVLQLNRKHAVAGF"
BASE COUNT          404 a          823 c          794 g          473 t
ORIGIN      
        1 tacacagcct ccccctgttc ttcctcatgg tcgtgattgc atgtttgcct tgcccggcgg
       61 gcttcgatcg cgacccagtt cagacagcac gaaaggagca cccatgcgga aagttacgat
      121 gtcactcctc gcctttgcgg cgatcgccct cacggcgacg attgcggctg cggatcgcgg
      181 cattcacgaa ggtccggtcg tcgggggcgc gaacgtcccg agcaaccagg aagtgctcta
      241 ccggagctat ggcgagtgca acgacgcctt cacctccggt atcaccatcc gcaccgtcgg
      301 cgggggcaac atcaccatcc cgccccaggt tgcggcgaac gcggtatggg ccgagatctt
      361 ctgggtcacc cttgccgacg cgcccgatgc gggtgcggtc accgtcaacg gcaacgcggt
      421 tgccccgctt gccaacggac cggtcacggt ttccccgtgc tggccgcagg actttgcgtt
      481 ctcgtaccgc gccaacgtgc ttcccttcat ccagggcggc gtcaaccagc tgcgcggatt
      541 cgcggagtcg ggtgtcgaca acgtctcccc gagcaccgag ggtgtcacga tggtggttgt
      601 ctaccgtacc aacacggccg acaaggaaat cgtcgtcctg ggcggcaatg acagcgccat
      661 cctgaaccag atcgatcttg ccattcccgt cgtctcggcc cccggcttcg gcgcggagct
      721 caccctggtc gtggccgatg gccagccggc cagcgacgtg gccctgtgga acggcctcgt
      781 gatttccgct ccggacgcgt tccagggcct tgatccgggc ccgggcatcg gatactggga
      841 cacccaggag tacggcgtgg ccaccggtgg tggaaacgtc gccagcgtca tcggccaggt
      901 cgattgcctc aactgggttg ccaccgtcct cggcgtgaag cgcggcgggt gcgtggtgcc
      961 ggtcgagccg tccacctgga gcaaggtcaa gaaccggttc aactgatccg gctcaaagca
     1021 agacagatcg gcggtccacc tccgggtggg ccgccgattt ttttgtctcc cgcatcctgt
     1081 ctgattcgcc ggtcgaaggg atgaggggcc tactcgtcgt cggtgaacga ctcgatccag
     1141 gcttcgcccg acgaggcgcg gccggtcacg tacagattgt tcggccggca gcagacccgg
     1201 cagtcctcga tgtactcctg cacttcaccg gccgacaggt cgacggtggt ctcgttggtt
     1261 tcgccgcagt aggcgcattc gaacgtggct tcgacgatca tccggggatt gtaccgggcc
     1321 cagggagccc gggggagcgg gggctcccct aaaaccccgc taccgcgtgc ttgcgattga
     1381 gctggagcac ccgggcatcc ggatcgacca ggatcgactt cggctcgaac gggcaggtga
     1441 gctgcacgtt caccttttca ccgggtgcgg gacggacggt cgtacgcacc tcgagatagg
     1501 ggtccttcga ctctttttcc gcggcatggg cggccggcgc ggcatccgat ccgggagccg
     1561 gcgcgccctt cctcttcgga aagcgctcac ccgtgatggc ggccacttcc accggcatga
     1621 caccggtgcc cgcgttggtc acctccaccg tcacttccca ggtgtcgccg acccgcttct
     1681 tcgtggcgcc ggccagcttg tactcgggca cgaccacctg gtggaaccac tggtcgacga
     1741 acgcatcgta ggcggtcgtg tcgggcgcga agccgcgcat cacctccacg taatcgggga
     1801 ggagcgcgtg gtcacgattg tgagcgtact tgtggatgaa ggcgctgttc cccgccaggg
     1861 ccgaatcacg ccccatctcg ttgaggagca tccagaagac ccagcccccc ttgtcgtagg
     1921 taaccgtctg gtcgccatcc ttgctgccgt cgatcttcac cagcggtcgc tccgagtcga
     1981 cctggcggcg ttcgccgtaa cggctctcga tccgtttcag gaactcgatc cgctgctccg
     2041 gccccttcac ctggtcgaac aggaggatcg tggagaagtg ggccattccc tccgaaagga
     2101 tgtcgccgct cggcccctcg cccggcatca ggatgtttcc ccaccactgg tgagccgctt
     2161 cgtgggccgt caccatgaac gccaggttgg agcggggatc gctcttggtg aggaagccga
     2221 tctgctcgga gaacgtgata tcggtgggga agccttgcgc gtatgccgac agcccgggga
     2281 attcggacag tttcagttcc tgccagggga acggatagaa ccactccgag aaatatttgc
     2341 gggcggcgtc gagcgcctcg gacatttcgg ccacgttgta gtcatgcccc gggtggtagt
     2401 agatcaccgt gccattgccg cgcttctcgg cccagcgcgc cgccaccacg ttgaagaagt
     2461 tgaccgggct gtcggactgc caggtcatcg tgcg
//
LOCUS       DASVOD010000077         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_57188, whole genome shotgun sequence.
ACCESSION   DASVOD010000077 DASVOD010000000
VERSION     DASVOD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57188"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1803
                     /locus_tag="VF720_01505"
     CDS             124..1803
                     /locus_tag="VF720_01505"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEX7878052.1"
                     /translation="MSPEREVLEVDVLFVGAGPAGLAGAYHLANLVEQGKAAGTIPAE
                     GLEIAVIEKAAEVGMHSLSGAVLDPRAIRELIPDFEAEGAPVESTVTGDEFYFLTDGG
                     QIKAPINPPPLQNHGNYVISLGNLTRWLAGRVEEKGVYVFTGFPGSELLYEGNRVNGV
                     RTGDKGIDKNGEKKPNYQPGTDIRAKVTILCEGARGSLTKQLIARHNLEPKDHPQVYT
                     TGVKEIWEMPEGRVTAGRVIHTLGFPLPADTFGGGFIYGMSRNRWAVGFVTGLDYRNP
                     FTDPHHEMQKFKTHPVVRKLIEGGKMVAYGAKAIPEGGWYAMPKPVVDGAMICGDAGG
                     FLNAQRLKGIHLAMKSGMLAAETALDAVRKNDFSESTLNAYRDRVNQSFIRKELWNVR
                     NFHQPYQQGLWTGLFHTGIQMITGGRGLTDPFPSQPGYARMEKVKGYFGRGDAKPEKI
                     KYDGRLTFDKLSDVYLSGTTHEENQPCHLKVADLNICATRCTAEFRNPCEHFCPAAVY
                     EPIRREDGSFERLQINFSNCVHCKTCDIADPYQIITWVTPEGGDGPNYQNL"
     gene            1800..>2259
                     /locus_tag="VF720_01510"
     CDS             1800..>2259
                     /locus_tag="VF720_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7878053.1"
                     /translation="MSRPARRPGRVLLLVTILLVAGMAAFANDGRRFFGSTRPPSKNI
                     LTYNNGAEPEVVDPGFSVGQPDGNVCRIQWEGLTVEHPSTLQPLPGMAERWDVSADGL
                     TYTFHLRRAEWSDGHPVTAQDFLWSWRRVIAPGSTARYAGLLFYVKNAEAY"
BASE COUNT          432 a          793 c          689 g          345 t
ORIGIN      
        1 ggaacaaagg catcatccgg ccggacctcc ctccggccgg atgatgcctt gattcgactc
       61 agtagcctgg catacgcccg accccatttc ggcccgcccc cgaattcact acaatcgggc
      121 cgcatgagtc ccgaacgcga agtcctcgaa gtcgatgtcc tgttcgtcgg cgccggtccc
      181 gccggcctgg cgggggcgta tcatcttgcc aacctcgtcg agcaggggaa ggcggccggc
      241 acgatccctg ccgaggggct cgagatcgcc gtcatcgaga aggcggccga ggtcggcatg
      301 cacagcctct ccggcgcggt gctcgatccc agggccatcc gcgagctgat cccggacttc
      361 gaggccgaag gcgcccccgt cgagagcacc gtcaccggcg acgagttcta cttcctcacc
      421 gacggtggcc agatcaaggc gccgatcaat cccccgccgc tccagaacca cggcaactac
      481 gtcatctccc tcggcaacct caccagatgg cttgccgggc gcgtcgaaga gaagggcgtt
      541 tacgtcttca ccgggttccc cggttcggaa ctgctgtacg aggggaaccg cgtcaacggc
      601 gtccgcaccg gcgacaaggg gatcgacaag aacggcgaga agaagccgaa ctaccagcct
      661 ggcaccgaca tccgcgccaa ggtcaccatc ctgtgtgaag gggcgcgcgg ttcgctgacc
      721 aagcagctga tcgccaggca caacctggag ccgaaggatc atccccaggt ttacacgacg
      781 ggcgtcaagg aaatctggga gatgcccgag ggtcgcgtga ccgccgggcg cgtcatccac
      841 acgctcggct tcccgctccc ggccgatacg ttcggcggcg gcttcatcta cggcatgagc
      901 cgcaaccgct gggcggtggg gttcgtcacc ggcctcgatt atcgcaaccc cttcaccgat
      961 ccccatcacg agatgcagaa gttcaagacg caccccgtgg taaggaagct gatcgagggc
     1021 ggcaagatgg tggcgtacgg cgccaaggcc attcccgagg gaggctggta cgccatgccg
     1081 aagcccgtgg tcgatggcgc catgatctgc ggcgacgccg gcggcttcct gaacgcgcag
     1141 cgcctcaagg gaatccacct ggcgatgaaa tccgggatgc tggccgcgga aaccgctctc
     1201 gacgccgtcc ggaagaacga cttctcggag agcacgctga acgcctaccg cgatcgcgtg
     1261 aaccagtcgt tcatccggaa ggagctctgg aacgtccgca acttccacca accctaccag
     1321 cagggtttgt ggaccggcct cttccacacc ggcatccaga tgatcaccgg tggccgcggc
     1381 ctcaccgacc ccttcccttc gcagcccggc tacgcgcgca tggaaaaggt gaaggggtac
     1441 ttcggccggg gcgacgcaaa gcccgaaaag atcaagtacg acggcaggct gaccttcgac
     1501 aagctgtcgg acgtgtacct ctcgggtacc acgcacgagg agaaccagcc gtgccacctc
     1561 aaggtggccg acctgaacat ctgcgccacc aggtgcacgg ccgaattccg aaatccctgt
     1621 gaacacttct gcccggccgc cgtctatgaa ccgatacgac gcgaggacgg ctccttcgag
     1681 cggctgcaga tcaacttctc caactgcgtc cactgcaaga catgcgacat cgccgacccc
     1741 taccagatca tcacctgggt cacgcccgag gggggcgacg ggccgaacta ccagaacctg
     1801 tgagtcggcc cgcgcgccga ccgggccggg tcctgctcct cgtcaccatc ctcctcgtcg
     1861 cgggcatggc cgcgttcgct aacgacggcc ggcgcttctt cggcagcacc cgccccccat
     1921 cgaagaacat cctgacctac aacaacggtg ccgagcccga agtcgtggac cccggcttct
     1981 cggtcggcca gcccgacggc aacgtctgcc gcatccagtg ggaagggctg accgtcgagc
     2041 acccgagcac gctgcagccg ctgccgggca tggccgagcg gtgggacgtg agcgccgacg
     2101 gtctcacgta cacgttccat ctccgcaggg ccgagtggag cgacgggcac cccgtcaccg
     2161 cgcaggattt cctctggtcg tggcgccgcg tcatcgcccc cggatcgacg gcccgctacg
     2221 cgggcctcct cttttatgta aagaatgccg aggcgtacc
//
LOCUS       DASVOD010000078         2357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_474870, whole genome shotgun sequence.
ACCESSION   DASVOD010000078 DASVOD010000000
VERSION     DASVOD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474870"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            21..971
                     /locus_tag="VF720_01515"
     CDS             21..971
                     /locus_tag="VF720_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878054.1"
                     /translation="MNFRVLPAFVLVTAGVASAAAPSWQARGPLSEPSLFGDGIISTG
                     QFESHPCFTPDGNTLYFVRSTPQFTDWKIWESHFADGKWSTPVMASFSGTHRDADPFI
                     TPDGARLYFISDRPVDGKAREDMDVWFMDRAPDGEWGEPRNMGAPVNSAGSEWYPRVA
                     AGGTLYFGSDRPGGEGATDLYRVRPTDGGYAAPENLGPSVNSAADEYEPCIAPDESFL
                     VFMAAGRPDSRGGGDLYISFREKEGWSAARNLGPLINRPAMEISASLSPDGQRFFFSS
                     CRRDTTVKSTPGERPDRSRNGLGDIYQMDLDALLKLGSGK"
     gene            complement(1024..>2357)
                     /locus_tag="VF720_01520"
     CDS             complement(1024..>2357)
                     /locus_tag="VF720_01520"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7878055.1"
                     /translation="PGPTIPGNSWTFVAVSVDRVAATGTWYLNGAPVPGFDFSPPSGS
                     LFNNADLLVGGRPGAPSWNGLIDELQFFGTPLSAQSIAAVAAAPAGKCREHCRVPAVT
                     SICKDKPTVTICMNIVNQTNAPQSYHWSVAGLPSGPGCSVAGPVTFSPSSGTIVVPAG
                     GISSPICITMTRPAGLINQNDTACFEFSFVNDATGVCRACVGKIRADNTCWCVTPAQP
                     GVVAVGQRFAPGITGQPIVIGIKHPCDPVAVIDYRVTAVYEPGAHPDPLAVSLNGLPP
                     GEPVLGSLSLDTEGETEVTVMATYPRAYDPAGLYQVVMEADTDGDGIMEVVSSTPIAS
                     TYEADAVSTVPDVAAPPVPLVSLKALPNPFQAKATLSVTLPVAGPVDLAVYDLSGRLI
                     RRVFAGSVAAGPSTFLWDGRDEAGRETGGGIYFVRLVSGGVRQQAKLVRVR"
BASE COUNT          404 a          732 c          841 g          380 t
ORIGIN      
        1 caagaaggag aagtagggtc gtgaattttc gggtgctgcc ggcgttcgtg ctcgttaccg
       61 cgggtgttgc ctccgccgcg gcaccctcct ggcaggcgcg tggcccgctg tccgagccga
      121 gcctgttcgg cgacgggatc atttccacgg gccagttcga atcgcacccc tgcttcacgc
      181 cggacggcaa caccctctac ttcgtgcgaa gcacgccgca attcaccgac tggaaaatct
      241 gggagtcgca cttcgccgac ggcaagtggt cgactccggt catggcttcg ttttccggca
      301 cgcatcgcga tgcggatcca ttcatcacgc cggatggggc gcggttgtat ttcatctcgg
      361 atcgaccggt ggatggaaaa gcgcgggagg acatggacgt ctggttcatg gaccgcgctc
      421 ccgacggtga atggggagag cctcgcaata tgggggcgcc ggtgaactcg gcgggaagcg
      481 agtggtaccc gcgcgtggcg gccggcggca ccctctactt cgggtcggat cggccgggcg
      541 gtgagggagc cacggatctc tatcgcgtgc ggccgacgga tggcggctat gctgcgccgg
      601 agaaccttgg cccgagcgtg aacagtgcgg cggacgagta cgagccctgc atcgcccccg
      661 acgagagttt cctggtcttc atggcggcgg gtcggcccga cagccgcggc ggtggcgatc
      721 tctacatcag cttccgggag aaggaggggt ggtcggctgc ccgcaacctc gggcccttga
      781 tcaatcggcc ggcgatggag atttcggcgt ccttgtcgcc ggatggtcag cgcttcttct
      841 tttcaagttg ccggcgggat acaaccgtca agtcgacgcc gggcgagcgg ccggaccggt
      901 cgcggaacgg gttgggagac atctaccaga tggacctgga cgcgctgctg aaactgggga
      961 gtgggaagta gatcgagctg ccgcgcggcc tcaaccaacg cggggcccac gactaagacc
     1021 gaattaccgc acccgcacga gcttcgcctg ctgccgcacg ccacccgaca cgagtcgcac
     1081 gaagtagatt ccgccgccgg tttcgcgacc ggcctcatcg cgtccatccc acaggaaggt
     1141 tgacgggccg gcggcaacgg aacccgcgaa gacgcggcgg atcagccgac cggacaggtc
     1201 gtacacggca aggtccaccg gtccggccac cggcagcgtg acgctcagcg tggccttcgc
     1261 ctggaacgga ttgggcagcg ccttgaggct cacgagcgga accggcggcg ctgcaacatc
     1321 gggcaccgtc gacaccgcgt cggcctcgta ggtcgacgcg atcggcgtgc tgctcaccac
     1381 ctccatgatg ccgtcaccgt cggtgtcggc ttccatcacc acctggtaga ggccggcggg
     1441 atcgtaggcg cgcgggtaag tggccatcac ggtgacttca gtttcgcctt ccgtgtcgag
     1501 cgagagcgaa ccgaggacgg gctcgccggg aggcaggccg ttgaggctta cggccagcgg
     1561 atcgggatgc gcgccgggtt cgtacacggc cgtcacgcga taatcgatga cggcgacagg
     1621 atcgcacgga tgcttgatgc cgatcacgat cggctggccg gtgatgccgg gggcgaatcg
     1681 ctggccgacg gccaccacgc cgggctgggc aggtgtcacg caccagcagg tgttgtcggc
     1741 gcggatcttg ccgacacacg cacggcacac cccggtggca tcgttgacaa acgagaactc
     1801 gaagcaggcg gtgtcgttct ggttgatgag gccggcgggg cgcgtcatcg tgatgcagat
     1861 cgggctcgag atgccgccgg cgggcacgac gatcgtgccg gacgatggcg aaaacgtgac
     1921 cggaccggcc acgctgcaac cggggcccga cggcaggccg gccaccgacc agtgatagct
     1981 ctgcggcgcg ttggtctggt tcacgatgtt catgcagatc gtgaccgttg gcttgtcctt
     2041 gcagatgctg gtgacggccg gcacgcgaca gtgttcgcgg cacttgccgg cgggggcggc
     2101 ggccacggcc gcgatggatt gcgccgagag gggcgtgccg aagaactgga gttcgtcgat
     2161 gaggccgttc cacgatggcg cgccgggccg gccaccgacc agcaggtcgg cattgttgaa
     2221 gagtgagccg ctcggcggtg aaaaatcgaa accgggcacg ggcgcaccat tgaggtacca
     2281 ggtgccggta gccgcgacgc gatccaccga cacggccacg aacgtccaac tgttgccggg
     2341 aatcgtcggg ccgggga
//
LOCUS       DASVOD010000079         9210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_631139, whole genome shotgun sequence.
ACCESSION   DASVOD010000079 DASVOD010000000
VERSION     DASVOD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631139"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VF720_01525"
     CDS             complement(<1..611)
                     /locus_tag="VF720_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HEX7878056.1"
                     /translation="MNPQLPVGWAGSEAFRKFAEGRRIDQAAIDAFLAGQPVPIVEGS
                     NCTFVWRGEADEVRLRHWIFGLSTAQPFMRLEGTDLWFLTMELPARSRVEYKLEVARG
                     DRVELLQDPLNPMTARDPFGANSVVVGTGYEVPEWTRYDEEARPGNLEELVVRSHAMR
                     RDQRVTLYLPARFRRTRRYPLLIVHDGGDFLAYSGMKTILDNL"
     gene            complement(608..1564)
                     /locus_tag="VF720_01530"
     CDS             complement(608..1564)
                     /locus_tag="VF720_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX7878057.1"
                     /translation="MSPDTVSLLGPQRFQRTLGDVLASRGIEGPVAVITAGWQEREHD
                     DAELRDHLGGRALNLELHTRTDRIFDRDPELAAAHHERQATLKSMQELYDLRLGHVME
                     GARQLLRRRGDLDVLGPERLEAVEDVRGLDRRHLERIRVVHAEFESRWNPGLRSAVMA
                     ERNEVEQMIQRSSAIAIAGGHVAVLLNRLRLFDIAALAKGKPVIAWAAGAMAVSERII
                     LFYDDPPHGPGNAEVLDEGLGLVTGIVPLPHAGTRLKLDDGSRVALFARRVFPAVAVT
                     LDPGARVDWKDGRAVAVPDDTFAGPRHAFQLQADGDIALVTA"
     gene            complement(1582..2850)
                     /locus_tag="VF720_01535"
     CDS             complement(1582..2850)
                     /locus_tag="VF720_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX7878058.1"
                     /translation="MARTGADPQLKEGIDMHVVFIEPSFPANQREFVRALKEVGATVT
                     GIGERPKESLDGALREWLFHYEQVPSVVDDASVLRVVQWIQEKARVDRLEAVVEAHIQ
                     AAARVRALAGIPGTSERTAFLCRDKPAMKEVLRAGGIPCAASDGVETADAAREFAASV
                     GFPVIVKPRAAAGASGTYRADTVEELEGVIRTTGLDRGNPAAIEEFIEGHEGFYDTIT
                     INGQVVHEFVSHYYPNVLEAMRNRWISPQFIATNRLDSAEGYDEVKELGRRVLPILGI
                     ETSATHMEWFAGPRGLKFSEIGCRPPGVRAWDLYAAGNDIDIYREWAAAICLGHPLQR
                     ASRRFSAGIIALRPERDGTIAGYSGIENIQRAFGSWIIDWHLPQEGTPTQPVEAGYMA
                     NAWIRMKHPDYDELRSMLDTIGQTVKVHAR"
     gene            complement(2831..4321)
                     /gene="glgA"
                     /locus_tag="VF720_01540"
     CDS             complement(2831..4321)
                     /gene="glgA"
                     /locus_tag="VF720_01540"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEX7878059.1"
                     /translation="MKPIAPALPALRIAFVTSELAPFAKAGGLADVSAALPAYLDRAG
                     HDVRPFVPLYQSINWDRYGLTPVEGCQGIGIRMGEATFTVDLMTGRLPGSTLDVYFVH
                     CPPLFGRPGIYTSDPDEHRRFLLLSRAAIECLQRMGFSPDILHVHDWATALVPLYLKT
                     VYAWDKLFAATRTVLTIHNIGYQGTFGTEILKDLNPIGTQYFDGDDLKNGRVNLLKTG
                     LVHAHQLTTVSPTYAREIQTPEFGYGLDGILRMRSENLEGILNGVDYEAWNPETDPHL
                     PAHYGPADLSGKGVCKEALLSAVGLAPTSQAPVVGIVSRLAHQKGLDLLFEPIPALLQ
                     RREFRFVVLGNGEPKYEEFFGNLVSRFPGRAAFYSGYHEKLAHLIEAGSDFFVMPSRY
                     EPCGLNQMYSLKYGTIPVVRKTGGLADTVTMYDPATGRGNGVVFEHFTAEGAYWALDT
                     AMNLYGEPRHRERMIRNGMAADFSWETEGARYVALYRRLVGGANRG"
     gene            4532..4795
                     /locus_tag="VF720_01545"
     CDS             4532..4795
                     /locus_tag="VF720_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011153779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX7878060.1"
                     /translation="MEFPADRLAALISRRADTMSGMPVFVGTRVPIQNLLDYLEDDDM
                     AGFLDNFPSVRQEHAEELARLARGGVNVLEDPAGRVRTETAED"
     gene            complement(4797..4928)
                     /locus_tag="VF720_01550"
     CDS             complement(4797..4928)
                     /locus_tag="VF720_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878061.1"
                     /translation="MIDAEASIHRQDSVEAPNGMLENCRIRGPKEANLAHCHDLMTG"
     gene            5162..6388
                     /locus_tag="VF720_01555"
     CDS             5162..6388
                     /locus_tag="VF720_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7878062.1"
                     /translation="MSNVIFVAPYFLETTLRFVAGAANLPGVNVGLISTDPESKLPPE
                     IRSRLAGHFRLDDCLNPKELIRAGHAFRHAWGSVHAMIAALEELQVPLAEAREGLGID
                     GMSAKAAHNFRDKSQMKEVLRANGLPCARHALAPSERSAFDFANSNGYPIVVKPPAGA
                     GARSTWRVNNADELTRALAANPPRDDRPVLLEEFVQGREHSFDSVMLNGKLVWHSISR
                     YSPTPLDVLENPWIQWAVLLPREIDGPEFDDIRLAAAKALKALGLKTGLTHMEWFRRN
                     DGSIAISEVAVRPPGAQFTSLISYAHDCDFYSAWPRLMALNEFAPPERKWSVGAAYLR
                     GQGQGHVTAIHGIDAANRAVGPLVVESKLPRPSQASSGSYEGEGYVILRHPETSVVEE
                     GLKTLVSTIRVELGSA"
     gene            6385..7602
                     /locus_tag="VF720_01560"
     CDS             6385..7602
                     /locus_tag="VF720_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010852059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7878063.1"
                     /translation="MNVLFVSPGFPPEMPFFTRGLASVGARVIGLGDQPEANLPAMTR
                     DNLGAYFRINGFADEAGMLAEIEKISRQVRIDRIECLWEPMMIPVARAREKLGIPGMT
                     AAETIPFRDKEVMKQVLDDAGIRTPRHANATTAGEVREAASKVGFPLVIKPIAGAGSA
                     DTYKCRSAEELEGVLSKMRHIPEVSVEEFIEGEDFTFDTICADGKVLFENICFYRPRA
                     LISRTNDWISPQTVAVRDVEADWLRSGREMGYAVLKALGFKTGFTHMEWYRKPDGEAI
                     FGEIGCRSAGARTVDVMNWAADTDSFRNWAEAVCHGRITAPLEKKYNAVAIFKRAEGE
                     GRIQRIEGLARLMNEFGQHICAIDLTPLGAPKRPASQTLIGDGFVMLRHPDLAAALEL
                     GDRVATELRLYAG"
     gene            7592..7945
                     /locus_tag="VF720_01565"
     CDS             7592..7945
                     /locus_tag="VF720_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878064.1"
                     /translation="MRGEALLAMLLGLAIGGAGCQSSKPAAQPPVSSTTPPPATEKGA
                     DTVTGAGTIVRLEIEGGFFAIRGDDGTNYDPKSLPDEFKVEGLRVRYTLKVNTGAAGI
                     HMVGPIVDVIDIAKE"
     gene            7959..8378
                     /locus_tag="VF720_01570"
     CDS             7959..8378
                     /locus_tag="VF720_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7878065.1"
                     /translation="MIVRAFLPADIPAVRELWSATDGLGFGPGDAHVELESFLARNPG
                     LSPVAVSDDVLVGALLCGHDGRRGCLYRLAVAASHRRRGVAGQLVRHALDGLRAAGIP
                     RALLYVLADNAPAAAFWKSIGGRIRGELDMFSIDLAP"
     gene            8375..8773
                     /locus_tag="VF720_01575"
     CDS             8375..8773
                     /locus_tag="VF720_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878066.1"
                     /translation="MTQRLAALALVVRDYDEAIAWFCDTLGFDLLEDTPLGNGKRWVR
                     VRPPGGNGAELLLARAGNEEQAAHIGNQTGGRVFLFLETDDLERDYRAWSDRGVRFVE
                     RPRTEMYGQVVVFEDLYGNKWDLIERRPAE"
     gene            8770..9093
                     /locus_tag="VF720_01580"
     CDS             8770..9093
                     /locus_tag="VF720_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7878067.1"
                     /translation="MTPAMFRKLALSFPDAVESSHQAHPDFRAHGKVFASLGYPDAGH
                     GSLKLTPAQQEAEMAQHPDVFSPAAGAWGRRGYTVVALKPATVTLLRRAMAAARANVE
                     PKKSR"
BASE COUNT         1493 a         3088 c         3098 g         1531 t
ORIGIN      
        1 atcaggttgt cgaggatcgt cttcatcccg gagtaggcca ggaagtcgcc gccgtcgtgg
       61 acgatgagca ggggatagcg gcgcgtgcgg cggaaacggg ccggcaggta gagcgtgacg
      121 cgctggtcgc gccgcatggc atggctccgg acgaccagtt cctcgaggtt gccggggcgg
      181 gcttcctcgt cgtagcgggt ccactcgggc acttcgtagc cggttccgac caccactgag
      241 ttggcaccga acggatcgcg ggcggtcatg gggttcagcg gatcttgtag aagctccacg
      301 cggtcgccgc gcgccacttc gagcttgtac tccacacggc tcctggccgg cagctccatc
      361 gtcaggaacc agaggtcggt tccctcgagg cgcatgaagg gttgcgcggt ggagaggccg
      421 aagatccagt ggcgcaggcg cacctcgtcc gcctcgcccc gccagacgaa cgtgcagttc
      481 gatccttcca cgatcggcac gggctgcccg gccaggaagg cgtcgatggc ggcctggtcg
      541 atccgccgtc cctcggcgaa cttgcggaac gcctccgaac cggcccagcc gaccgggagc
      601 tgcgggttca cgcggtcacc agggcgatgt cgccgtcggc ctgcagctgg aacgcgtggc
      661 gcgggccggc aaaggtatcg tccggcacgg ccacggcgcg cccgtccttc cagtcgacgc
      721 gggcgcccgg gtcgagcgtg acggccacgg cgggaaaaac gcgccgggcg aagagggcca
      781 cgcgcgagcc gtcgtcgagc ttgaggcgcg taccggcgtg gggaaggggg acgatgccgg
      841 tgacgaggcc caatccctcg tccagcacct cggcgttgcc cggcccgtgc gggggatcat
      901 cgtagaagag gatgatccgt tcgctcaccg ccatcgcgcc ggccgcccag gcgatgaccg
      961 gctttccttt cgcgagcgcg gcgatgtcga agagccggag ccggttcagc aggaccgcca
     1021 cgtgcccgcc tgcgatggcg atggccgagg agcgctggat catctgctcg acctcgttcc
     1081 gttcggccat cacggccgaa cgcagcccgg ggttccagcg cgattcgaac tcggcatgca
     1141 ccacccggat ccgctccagg tggcgacgat ccaggccccg cacgtcttcc accgcctcga
     1201 ggcgctcggg tccgaggacg tccaggtcgc cgcgccggcg gaggagctgg cgcgcccctt
     1261 ccatcacatg gccgagccgg agatcgtaca gctcctgcat cgacttgagc gtggcctgtc
     1321 gctcgtgatg ggcggccgcg agctcgggat cgcgatcgaa gatgcggtcg gtgcgggtat
     1381 ggagctcgag gttcagcgcg cggccaccca ggtggtcgcg gagctccgcg tcatcgtgtt
     1441 cccgctcctg ccagcccgcg gtgatgacgg ccaccggccc ctcgatgccg cgcgaggcca
     1501 gcacgtcgcc cagggtgcgc tggaagcgct ggggcccgag gagcgagacg gtgtccggtg
     1561 acacggcgga cgcggtcccg gtcaacgcgc gtgcaccttc acggtctggc cgatcgtgtc
     1621 gagcatgctc cggagctcgt cgtagtcggg atgcttcatc cggatccagg cgttggccat
     1681 gtagccggct tcgaccggct gcgtgggcgt tccctcctgc ggaagatgcc agtcgatgat
     1741 ccacgagccg aaggcccgct ggatgttctc gatgcccgaa tagccggcaa tcgtgccgtc
     1801 ccgctcggga cgcagcgcga tgatcccggc cgagaagcgg cggctggccc gctgcagcgg
     1861 atgcccgagg cagatcgccg cggcccattc gcggtagatg tcgatgtcgt tgccggccgc
     1921 gtagaggtcc caggcgcgca cgcccggcgg gcggcagccg atctcggaga acttgagccc
     1981 cctggggccc gcgaaccatt ccatgtgcgt ggcgctcgtc tcgatgccga ggatggggag
     2041 gacgcgtcgc cccagctcct tcacttcgtc gtatccctcc gccgaatcga gccggttcgt
     2101 ggcgatgaac tgcgggctga tccagcggtt gcgcatggcc tcgaggacgt tggggtagta
     2161 gtgcgagacg aactcgtgaa cgacctgccc attgatggtg atcgtgtcgt agaaaccctc
     2221 gtgcccctcg atgaactcct cgatggccgc cggattgccg cgatcgaggc ctgtggtgcg
     2281 aatcactcct tcaagctctt cgacagtgtc ggcgcggtaa gtccccgaag cgcccgcggc
     2341 cgcccggggc ttcacgatca ccgggaaccc gacggaggcg gcgaattcac gggcggcatc
     2401 ggccgtttcg acaccatcgg aggcggcgca gggaatgccg cccgcccgga gcacttcctt
     2461 catggccggc ttgtcgcggc agaggaacgc cgtccgctcg ctcgtgcccg ggatgccggc
     2521 cagggcgcgt acgcgcgcgg cggcctggat gtgcgcctcg accaccgctt ccagccgatc
     2581 gacacgcgcc ttctcctgga tccactggac gacgcgcagc accgaggcgt cgtcgaccac
     2641 ggagggaacc tgttcgtagt ggaagagcca ctcgcgcagc gccccgtcga gcgattcctt
     2701 cggccgctcg ccgatgccgg tgacggtggc cccgacttcc ttcagggcgc gcacgaattc
     2761 gcgctggttg gccggaaagc tcggttcgat gaagacgacg tgcatgtcga tcccctcttt
     2821 cagctgcgga tcagccccgg ttcgcgccac ccacgaggcg ccgatacagc gccacgtagc
     2881 gcgccccttc cgtctcccac gaaaaatcgg ccgccatccc gttccggatc atccgctccc
     2941 ggtgacgcgg ctctccgtac aggttcatgg cggtatccag cgcccagtac gcaccctcgg
     3001 ccgtgaagtg ctcgaagacg acgccgttcc cccggcccgt ggccgggtcg tacatcgtca
     3061 ccgtgtcggc cagcccgccc gtcttccgga cgaccgggat ggtgccgtac ttcaggctgt
     3121 acatctggtt caggccgcac ggctcgtagc ggctcggcat cacgaagaag tcgctccccg
     3181 cctcgatcag gtgggccagc ttctcgtgat atcccgagta gaaggccgcc cgccccggaa
     3241 agcggctcac aaggttgccg aagaactcct cgtacttcgg ttcgccgttg ccgagcacca
     3301 cgaagcggaa ttcgcggcgc tgaagcagcg ccggaatcgg ttcgaacaac aggtcgagcc
     3361 ccttctggtg ggcgagacgg ctcacgatgc ccaccaccgg cgcctgcgac gtcggcgcga
     3421 ggccgaccgc actgagcagc gcctccttgc acaccccctt tccggataga tccgccggcc
     3481 cgtagtgggc cggaaggtgg ggatcggttt ccggattcca cgcctcgtaa tcgacgccgt
     3541 tcaggatccc ttccaggttc tccgaccgca tccggaggat gccatcgagc ccgtaaccga
     3601 actccggggt ctggatctcg cgcgcgtagg tcgggctcac cgtcgtgagc tggtgcgcgt
     3661 gcacgagccc cgtcttgagg aggttcacgc gtccgttctt caggtcgtcc ccgtcgaagt
     3721 actgggtgcc gatcgggttc aggtctttca ggatctcggt gccgaacgtc ccctggtacc
     3781 cgatgttgtg gatggtgagc accgtgcgcg tggcggcgaa gagcttgtcc caggcataca
     3841 ccgtcttgag atacagcggc accagcgcgg tggcccagtc gtgcacatgg aggatgtccg
     3901 gcgaaaaacc catccgctgc aggcattcga tggccgcgcg cgagaggagc aggaaccgcc
     3961 ggtgttcatc cggatcgctg gtgtagatgc ccggccgccc gaagagggga gggcagtgca
     4021 cgaagtacac gtccagcgtc gagcccggca gccgacccgt catgagatcc accgtgaagg
     4081 tggcctcgcc catgcggatg ccgatcccct ggcacccctc gaccggcgtc agcccgtacc
     4141 gatcccagtt gatgctctgg tagaggggca cgaacggccg cacatcgtgt cccgcccggt
     4201 cgagatacgc gggcagcgcc gccgacacgt cggccagccc accggccttg gcaaacggcg
     4261 ccagctcgga cgtgacgaac gcaatacgga gcgccggcaa agccggcgcg atcggtttca
     4321 tgtttgccgg aatcggcgcg gatgccggtg caacaccgac gggtgcgcca gccggtgctt
     4381 tcgtccgtga cgaacggggt ttcgacatgg ggcggggagg ataacaccgg aagggggcgt
     4441 tctgggtgtc ggatcggggt ccggcacctc ttcagaaaaa ggtgccagtt ccccttgcgg
     4501 agtcgaaccg cccgtgtcct atgatcttcc catggaattt ccagccgatc gtctggcggc
     4561 gctgatttcg cgtcgcgcgg acacgatgag cggcatgccg gtcttcgtgg gtacgcgagt
     4621 gcctatccag aatcttctcg attatctgga ggacgacgac atggctggat tcctcgacaa
     4681 cttcccctcc gtccgacagg agcacgctga ggaactggca cgattggcca ggggcggcgt
     4741 gaatgtgctt gaggatcctg ctggacgagt gcgtaccgag actgctgaag actgagctac
     4801 ccggtcatca ggtcatgaca gtgcgcgaga ttggcctctt tggggccccg gatacgacag
     4861 ttctcaagca tgccgttggg cgcttcgacg ctatcctgac ggtggatcga cgcttcggcg
     4921 tcgatcatcc gatccctccc accggcgcga tcattgtgct attgagagcc aggtcaagcc
     4981 gagtcctcga cctcaagccc ttactccaaa aggtgatgca agtgctcgta tcgacacccc
     5041 cggggcgacg tgtcgaagtc tgggcgccat ccgcaagctg aatccccctc gacttggacc
     5101 aatcccccca cagggataga ctcctgcctc gttcgattgt ccaacggagg cctgcccctt
     5161 catgtccaac gtcatcttcg tcgctcccta ctttctcgag acgacgcttc gcttcgtggc
     5221 cggcgcggcc aacctgcccg gcgtgaatgt cgggctcatc agcacggatc cggagtcgaa
     5281 gttgccgccg gagatccgca gtcgcctggc cgggcacttc cggctcgatg attgcctgaa
     5341 tccgaaggag ttgattcggg ccgggcatgc gttccggcat gcctggggat cggtgcacgc
     5401 gatgattgcg gcgctcgagg aactccaggt gccgctggcc gaggcgcgcg aagggctcgg
     5461 gatcgacggc atgtcggcga aggcggccca caacttccgg gacaagtcgc agatgaagga
     5521 agtgctccgc gcgaacggcc tgccgtgtgc gcgtcatgcg ctggcgcctt cggagcggag
     5581 cgcgttcgat ttcgccaact cgaacggtta cccgatcgtg gtgaaaccgc cggcgggagc
     5641 cggcgcccgc agtacctggc gcgtgaacaa tgccgacgag ctcacgcgcg cgctggccgc
     5701 caatccgccc cgtgacgatc gcccggtgct gctcgaagag ttcgtccagg ggcgcgagca
     5761 ctccttcgat tcggtgatgt tgaacggcaa gctggtgtgg cattccatca gccgttactc
     5821 gccgacgccg ctcgacgtgc tcgagaaccc gtggatccag tgggcggtgc tcctgccgcg
     5881 cgagatcgac gggccggaat tcgacgacat ccgcctggcc gcggcgaagg ccctgaaggc
     5941 cctgggcctc aagaccgggc ttacccacat ggaatggttt cgtcggaatg acgggtcgat
     6001 cgccatctcg gaagtggccg tccgcccgcc gggggcgcag ttcacgtcgc tcatctcgta
     6061 tgctcacgat tgcgatttct actccgcgtg gccgcggctg atggcgctga acgagttcgc
     6121 gccgccggaa cggaagtggt ccgtcggcgc ggcctatctg cgcgggcagg ggcaggggca
     6181 tgtaacggcc attcatggaa tcgacgcggc caaccgggcc gtgggaccgc tcgtcgtcga
     6241 atcgaaactg ccgcggccct cgcaggcttc gagcggcagt tatgaaggcg aggggtacgt
     6301 catccttcga catccggaaa cgtcggtggt ggaggagggg ctcaagacgc tcgtctccac
     6361 cattcgcgtg gaattggggt ccgcatgaac gttctcttcg tctcgcccgg ctttccgccg
     6421 gaaatgccct tcttcacccg cggcctggcc tcggtcggcg cgcgcgtcat cggcctcggc
     6481 gaccagccgg aggccaatct cccggccatg acgcgggaca accttggcgc ctacttccgc
     6541 atcaacggat ttgccgacga agcgggaatg ctggccgaga tcgagaagat ctcccggcag
     6601 gtccgaatcg accggatcga atgcctctgg gaaccgatga tgattcccgt ggcgcgcgcc
     6661 cgcgagaaac tcggcatccc cggcatgacg gccgccgaaa cgattccgtt ccgcgacaag
     6721 gaagtgatga agcaggtgct cgacgatgcc ggcatccgca cgccgcgcca cgccaacgcc
     6781 accacggccg gcgaggtgcg cgaggcggcg agcaaggtcg gctttccgct ggtcatcaag
     6841 ccgatcgccg gcgcgggctc ggccgacacg tacaagtgcc gttccgccga ggaactggag
     6901 ggcgtgctct cgaagatgcg gcacatcccc gaggtgagcg tggaggagtt catcgaggga
     6961 gaagacttca ccttcgatac catctgcgcc gacggcaagg tcctcttcga gaacatctgc
     7021 ttctatcgcc cgcgcgcgct catcagccgc accaacgact ggatcagccc gcagaccgtg
     7081 gcggtgcgcg acgtggaagc cgactggctc cgctccggcc gggagatggg gtacgcggtc
     7141 ctcaaggcgc tcggcttcaa gaccggcttc acccacatgg agtggtaccg gaagcccgac
     7201 ggcgaggcca tcttcggcga gatcggctgc cgctcggccg gcgcccgtac cgtggacgtg
     7261 atgaactggg ccgccgacac cgacagcttc cgcaactggg ccgaggccgt ctgccacgga
     7321 cgcatcaccg cgcccctcga gaagaaatac aacgccgtcg ccatcttcaa gcgggccgaa
     7381 ggggaaggac gcatccagcg catcgaaggc ctggcgcgcc tgatgaacga gttcggccag
     7441 cacatctgcg ccatcgacct cacgccgctc ggcgcgccga aacgcccggc ctcgcagacg
     7501 ctcatcggcg acggcttcgt gatgctccgg caccccgacc tggcggcggc cctcgagctg
     7561 ggggatcgcg tggcgacgga gttgcggttg tatgcggggt gaggcgctcc ttgcgatgct
     7621 gctcgggctg gccattggcg gcgccggttg ccagtcgtcg aagcccgcgg cccagccgcc
     7681 cgtgtcgagt accacgccac cgcccgcaac cgagaaaggc gccgacaccg tgaccggcgc
     7741 cggcacgatc gtgcgcctgg agatcgaggg tggcttcttc gccatcaggg gtgacgacgg
     7801 cacgaactac gatccgaagt cgctcccgga cgaattcaaa gtggaaggcc tccgcgtgcg
     7861 ctatacgctc aaggtgaaca ccggcgcggc cggcatccac atggtcgggc cgatcgtcga
     7921 cgtgatcgac atcgcgaagg aatgacgcgg cgggcaacat gatcgtccgc gcgtttcttc
     7981 ccgccgacat tccggccgtg cgcgaactct ggagcgccac cgacggcctc ggtttcggcc
     8041 cgggcgatgc gcacgtcgag ctcgagtcgt tcctggcccg caatcccggg ctttcgccgg
     8101 tggcggtatc cgatgacgtc cttgtcggtg ccttgctctg tggacacgac gggcggcgcg
     8161 gttgcctgta ccggctggcg gtcgccgcat cgcatcgtcg gcgcggcgtg gccggacaac
     8221 tggtgcgcca cgcgctcgac ggcctgcgcg ccgcgggcat tccccgggcc ttactgtacg
     8281 tgctcgccga caacgcccca gcggctgcct tctggaagtc gatcggcggt cgcatccggg
     8341 gggaactcga catgttctcc atcgacctcg ccccatgacg cagcgactcg ctgccctcgc
     8401 gctcgtcgtc cgcgattacg acgaagccat cgcctggttt tgcgacacgc tcggtttcga
     8461 tctcctcgag gacacgccgc tcgggaacgg caagcggtgg gtgcgggtgc gcccgcccgg
     8521 cgggaacggg gcggagttgc tactggcccg ggcaggcaac gaggagcagg cggcgcacat
     8581 cggcaaccag acaggcgggc gcgtcttcct ctttctcgag accgatgacc tcgaacggga
     8641 ttatcgcgcg tggagtgatc ggggcgtccg gttcgtcgag aggccgcgaa ccgaaatgta
     8701 cgggcaggtc gtcgtcttcg aggatctcta cggtaacaag tgggacctga tcgagcggag
     8761 gccggcagaa tgacgccagc catgtttcga aagctggcgc tctcgttccc ggacgcggtg
     8821 gagtcgtcgc accaggcgca tccggacttt cgcgcgcacg gcaaggtctt cgcgagcctg
     8881 ggctacccgg atgccggaca cggatcgctc aagctgacgc cggcccagca ggaagccgag
     8941 atggcccagc atccggatgt cttctcgccg gccgccggcg cctggggccg gcgcggctac
     9001 accgtggtgg cactgaagcc tgccacagtc acactcctgc ggcgggcgat ggccgcggct
     9061 cgggccaacg tggagccgaa gaagtcacgc tgaccggcgt gctacgatcg tcgcgtggag
     9121 gccttccgtt gacccgagtc ccatcccgcc gagccgctcc ttccgcggcc acgactatcc
     9181 cggccactcc cgcccccatc ctctccgacg
//
LOCUS       DASVOD010000080         5254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_944491, whole genome shotgun sequence.
ACCESSION   DASVOD010000080 DASVOD010000000
VERSION     DASVOD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944491"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /gene="infB"
                     /locus_tag="VF720_01585"
     CDS             <1..1896
                     /gene="infB"
                     /locus_tag="VF720_01585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX7878068.1"
                     /translation="DRKKKRSSVDDRAVKESVKKTMANMGAAGRRPKRRRVREDGTVI
                     VEDEKVVRVNEFITISELADHLEVKPTEVIAACMRLGVLATINQRLDRTTIEAVSDEF
                     GFGVEFAEEDEEEILESQVEDTDEQLLPRAPVVTIMGHVDHGKTSLLDYIRRTNVVAG
                     EVGGITQHIGAYEVTLDGGQQLTFLDTPGHEAFTTMRARGAQATDIVVLVVAADSRVM
                     PQTIEAINHARAADVPIIVAINKVDLPDANPTLIKQELAGQNVLVEEYGGKTLFAEVS
                     AKKGMNVEKLLELILLQAEVLELTANPHKAASGVVIESKIDKGRGIVATVLVQEGTLR
                     VGDPFVCGTQYGRVRALANERGQRIKEAGPSTPAEILGWSGTPEAGDVFQATATDQRA
                     REIAQERSMRQREAVRRRQRVSLVNFHEQIQAGQRAELPIVLKADVAGSVEVLTEQLG
                     KLGNEQVGCKVIHAGVGLITESDVNLAVASNGIVIGFHVRPEPRAAELARREKVDVHL
                     YEIIYEVIEQVKGALSGLLKPDEVEETQGVAEVRQVFSISKTGNIAGCMVTSGTITRT
                     SRVRLKRDGEVLTTGRLSSLKRFKDDVKEVVAGFDCGMAIEGYNDIQAGDVIEAFTVK
                     TVARKID"
     gene            1910..2206
                     /locus_tag="VF720_01590"
     CDS             1910..2206
                     /locus_tag="VF720_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX7878069.1"
                     /translation="MSFFVGVVRVELFIPQSRSLKDKRSVLHGVKDRLKALNAAVAEV
                     DGQDLWQRATLAVSLVSSDSGWIEKSVQDIRRIAERDDRALVSGFDWDVTPAPW"
     gene            2217..2594
                     /gene="rbfA"
                     /locus_tag="VF720_01595"
     CDS             2217..2594
                     /gene="rbfA"
                     /locus_tag="VF720_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX7878070.1"
                     /translation="MSRNPRAFRLADQIQFEVSDILENKLRDPRRGFLTITGVEVTDD
                     LRSAKIFVSALGPPEELTKAVELLQHARGFVRSELARRIRVRFMPEIHFRPDDSATRG
                     VEMDKLLRDLRDGKLPPDEDLES"
     gene            2678..3571
                     /gene="truB"
                     /locus_tag="VF720_01600"
     CDS             2678..3571
                     /gene="truB"
                     /locus_tag="VF720_01600"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7878071.1"
                     /translation="MLLVDKPAGPTSRATVDAIVRRFGRRDIGHAGTLDPFATGLLLV
                     LVGRATRLVPWTQEWPKRYRAMIRFGEGTDTLDYTGTVTSRGDVPEDLLARLPAAIAA
                     LTGEIRQAPPMVSAAKVGGRRLHELARAGEIVERKERERVVHAFDIIGFNSPDLEVDV
                     TCSSGTYVRVLAESLGGALGVPAHLAGLRRTAIGPWPVEQALAPDAIEALTPPELVAR
                     TLIAPGGILVDWPTHEASGTDAIDIGHGRIPGVWTIDPPRFDRCRIVRDGSLLALVER
                     NDGAFRFLRVFAPAEPGNGLP"
     gene            3568..4485
                     /locus_tag="VF720_01605"
     CDS             3568..4485
                     /locus_tag="VF720_01605"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX7878072.1"
                     /translation="MTSHHRRPPAAVTIGVFDGVHLGHRALVERTVAGAAALGGEAVA
                     VTFDPHPAAVLAPEHVPPFLTDPTEKTGRLIEAGADRVAVLSFTRELASLTPEEFVEG
                     HLARLASLKLLVIGPDFALGRGRVGNAERLAAIGVERGFTVEVVPARLDGNEVISSSR
                     IRRLVQRGEVRGAARLLGRAPSVSGTVVTGDGRGRTIGVPTANLAVPVERCRPAAGVY
                     AVRVRVEGESRPAHPGVMNVGNRPTFDGTELRFEVHRLDWSGEAVGRTWSVELVERLR
                     EERRFDRVEALVTQIRSDIETARQLLAAQ"
     gene            4666..4935
                     /gene="rpsO"
                     /locus_tag="VF720_01610"
     CDS             4666..4935
                     /gene="rpsO"
                     /locus_tag="VF720_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7878073.1"
                     /translation="MTLNRDQKQSIIGQYRLHDSDSGSPEVQIALLTEKIKYLTEHFK
                     IHKTDHHSRRGLLRMVGHRRRLLDYLKSTKIDRYRAIVKELGLRK"
     gene            5051..>5254
                     /locus_tag="VF720_01615"
     CDS             5051..>5254
                     /locus_tag="VF720_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018392394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878074.1"
                     /translation="MRQTFSREIAGRTLSIEVGHVARQAGGSAWVRFADTVVLAAAVA
                     EKEGKADRDFFPLTVDYREKMYAA"
BASE COUNT          904 a         1791 c         1763 g          796 t
ORIGIN      
        1 gatcgcaaga agaagcgttc ctcggtagac gatcgggcgg tcaaggagag cgtcaagaag
       61 acgatggcca acatgggcgc cgccggccga cgccccaagc gacgccgcgt ccgggaggac
      121 ggcaccgtca tcgtcgagga cgagaaggtc gtccgggtca acgagttcat cacgatcagc
      181 gagctggccg accatctcga ggtgaagccg accgaggtga tcgccgcctg catgcgcctc
      241 ggcgtcctgg ccaccatcaa ccagcgcctg gaccgcacca ccatcgaagc cgtgtcggac
      301 gagttcggct tcggcgtgga attcgccgag gaggacgagg aagaaatcct cgagtcgcag
      361 gtcgaggata ccgacgagca gctgctgccg cgggcgccgg tagtcacgat catgggccac
      421 gtcgaccacg gcaagacatc gctcctcgac tacatccgcc gcaccaacgt ggtggcgggc
      481 gaagtcggcg gcatcactca gcacatcggc gcgtacgaag tgacactgga cggcgggcag
      541 cagctcacct tcctcgacac ccctggccac gaagccttca ccaccatgcg cgcccgcggc
      601 gcccaggcca ccgacatcgt cgtgctggtc gtggccgccg acagccgcgt gatgccccag
      661 accatcgagg ccatcaatca cgcccgggct gccgacgtgc cgatcatcgt ggccatcaac
      721 aaggtcgacc ttcccgacgc caatcccacg ctcatcaagc aggagctggc cggccagaac
      781 gtgctggtcg aggagtacgg cggcaagacg cttttcgccg aagtctccgc caagaagggg
      841 atgaacgtgg agaagctcct cgagctcatc ctcctccagg ccgaagtgct cgagctcacc
      901 gccaatccgc acaaggcggc ctcgggcgtc gtgatcgagt ccaagatcga caaggggcgc
      961 ggcatcgtgg ccacggtcct cgtgcaggaa ggaacgctcc gcgtgggcga tccattcgtg
     1021 tgcggcaccc agtatggccg cgtgcgcgcc ctggccaacg agcgtggcca gcgcatcaag
     1081 gaagccggcc cctcaacgcc ggccgaaatc ctcggctggt cgggcacgcc ggaggccggc
     1141 gacgtcttcc aggccaccgc caccgaccag cgcgcccgcg agatcgccca ggagcgctcg
     1201 atgcggcagc gcgaggcggt tcgccgccgt cagcgcgtca gcctcgtgaa cttccacgag
     1261 cagatccagg ccggccagcg ggccgagctc ccgatcgtcc tgaaggccga cgtggccggc
     1321 tcggtggaag ttctcaccga gcagctcggc aaactcggca acgagcaggt cggttgcaag
     1381 gtcatccatg ccggcgtcgg cctcatcacc gaatccgacg tgaacctggc cgtggcctcg
     1441 aacggcatcg tcatcggctt ccacgtccgc ccggaaccgc gcgccgccga actggcccgg
     1501 cgcgagaagg tcgacgtgca cctgtacgaa atcatctacg aggtgatcga gcaggtgaag
     1561 ggcgccctgt cgggcctgct caagccggac gaggtggagg agacgcaggg cgtggccgaa
     1621 gtgcgccagg tcttcagcat ctccaagacc ggcaatatcg ccggctgcat ggtgacgagc
     1681 ggcacgatca cccgcaccag ccgcgtccgc ctgaagcggg acggcgaggt gctcaccacc
     1741 ggccgcctct cctcgctcaa gcgcttcaag gacgacgtga aggaagtcgt ggccggcttc
     1801 gactgcggca tggccatcga ggggtacaac gacatccagg ccggcgacgt gatcgaggcg
     1861 ttcaccgtca agaccgtcgc caggaagatc gactgagccg gggcccggca tgtcgttctt
     1921 cgtcggtgtc gtgcgcgtgg agctcttcat cccccagagc cggtcgttga aggacaagcg
     1981 atcggtcctg cacggggtca aggaccggct caaggcgctc aacgccgcgg tggccgaggt
     2041 cgatggccag gatctctggc agcgggccac gctggcggtg tccctcgttt cgtccgattc
     2101 cggttggatc gagaagagcg tgcaggacat ccgtcggatc gccgagcgtg atgatcgggc
     2161 gctggtgtcc gggttcgact gggacgtcac cccggcgccc tggtgaggtc cgaacgatga
     2221 gtcgcaatcc gcgtgccttc cggctggccg accagatcca gttcgaagtg tccgatatcc
     2281 tcgagaacaa gctgcgcgat ccccgtcgcg gcttccttac catcacgggc gtcgaggtca
     2341 ccgacgacct gcgcagcgcc aaaatcttcg tctcggcgct cggcccgccg gaagaactga
     2401 ccaaggcggt cgagttgctc cagcatgccc ggggcttcgt ccggagcgag ctggcccgtc
     2461 gcatccgcgt gcggttcatg ccggaaattc acttccgtcc ggatgattcg gccacgcgcg
     2521 gcgtcgagat ggacaagctg ctgcgggacc tgcgcgacgg caagctcccg ccggacgagg
     2581 acctggagag ctgatcgtga ccgcccggcc gccggacgcc cgagcgttgc ggcgcggaac
     2641 ctcccggcca cctcgtccgc gcgtggattc ggacggcatg ctcctcgtcg acaagccggc
     2701 cggccccacc tcccgcgcga ccgtcgacgc catcgtccgc cgcttcggcc gccgcgacat
     2761 cggtcacgcg ggcacactcg atccgttcgc caccggactc ctcctcgtcc tcgtcggccg
     2821 ggccacgcgc ctcgtgccct ggacgcagga atggcccaag cgctatcgcg cgatgatccg
     2881 attcggcgag ggtaccgaca cgctcgatta caccggcacg gtcacctccc gcggcgacgt
     2941 gccggaagac cttttggcgc gtcttcctgc cgcgatcgcc gcccttacgg gggagatccg
     3001 gcaggcgccc ccgatggtgt cggccgcaaa ggtgggaggg cgacgactgc acgagctggc
     3061 tcgcgccggc gaaatcgtcg agcgcaagga acgcgaacgc gtcgtccacg ccttcgacat
     3121 catcggcttc aattcacccg acctcgaagt cgacgtgacc tgctcgtccg gcacgtatgt
     3181 gcgcgtcctc gccgaatcgc tgggcggggc actcggcgtt ccggcgcacc tcgccgggtt
     3241 gcggcggacg gccatcggcc cgtggccggt ggagcaggcg ctcgcgccgg atgcgatcga
     3301 agccctgacg cctcccgagc tggttgcgcg gacactcatt gctcccggcg ggatcctggt
     3361 cgactggccg acgcacgagg cctccggtac cgacgccatc gacatcggcc acggccggat
     3421 cccgggggtc tggaccatcg atccgccccg attcgatcgc tgccgcatcg tgcgtgatgg
     3481 atccctcctc gcgctggtcg agcggaacga cggtgccttc cggtttctcc gggtattcgc
     3541 gccggccgag cccgggaacg gattgccatg acgagtcacc atcggcgccc gcccgcggcc
     3601 gtgacgatcg gcgtgttcga cggcgtacac ctcgggcacc gcgcgcttgt cgagaggacg
     3661 gtggccggcg cggcggcact cggcggcgag gcggtggccg tcacgttcga tccgcacccg
     3721 gcagcggtcc tcgcgcccga gcacgtgccc ccgttcctca ctgatccgac cgagaagacg
     3781 ggtcgcctca tcgaggccgg cgccgatcgc gtggccgtcc tctcgttcac ccgggagctg
     3841 gcctcgctca cgccggagga gttcgtcgag ggtcacctgg cccggctggc ctcgctgaag
     3901 ctcctggtca tcggccccga cttcgcgctg gggcgcggcc gcgtcgggaa cgccgaacgc
     3961 ctggccgcca tcggcgtcga acgcggcttc acggtggagg tcgtgccggc gaggctcgac
     4021 ggcaacgagg tgatctcgag cagccgaatt cgacggctcg tacaacgggg cgaggtgcgc
     4081 ggggcggccc gactactggg tcgggccccc tcggtatccg gaaccgtcgt gacgggggac
     4141 ggccgcggga ggacgatcgg agttccgacc gccaacctgg cagtaccggt ggagcgctgc
     4201 cggcccgccg ccggtgtcta tgccgtccgg gttcgggtgg aaggggaatc caggcccgca
     4261 catcccgggg tcatgaacgt cggcaaccgg ccgacatttg acggcacgga gctccgcttc
     4321 gaggtgcacc ggctcgactg gtcgggagag gcagtcggac ggacctggtc ggtggagctc
     4381 gtcgagcgcc tgagggagga gcgccggttc gatcgcgtcg aggcgctggt gacccagatc
     4441 cgatccgaca tcgagacggc ccgtcaactt cttgccgcac agtgatttga actcaggcgc
     4501 acggtggcac ttggcccgcg ccaggcccgg atgctatcct cccccggctc tttttccatg
     4561 gagatgtcga ccggctgagt gacgccggca gcatctcgaa ctccgtcgct ggaccccgga
     4621 cccggggcgg agaggatgac ttggccgggg aggatcattt ccatcatgac gctgaacagg
     4681 gaccagaagc agtcgatcat cgggcagtac cgtcttcacg attccgactc cggttcgccg
     4741 gaagtccaga tcgccctcct gaccgaaaag atcaagtacc tcaccgaaca cttcaagatt
     4801 cacaagaccg atcaccactc gcgccgcggc ctgctccgca tggtcggcca ccggcggcgc
     4861 ctgctcgact atctcaagtc gaccaagatc gatcggtacc gcgccatcgt caaggaactc
     4921 ggcctgcgca agtaggccgc gttcacgatg tcgcaggggg gaacgcgccg tggagtcgcg
     4981 gcctgccgcc ccattcgaat gcctacacga gagaagtcca aatagatgag aaagaggagg
     5041 taagaccagg atgcgtcaaa cattcagcag ggagatcgcc ggccgaacgc tgtcgatcga
     5101 ggtcggacat gtcgcacgcc aggcgggagg atcggcctgg gtgcgctttg ccgacacggt
     5161 cgtactggcg gcggcggtgg cggagaagga gggaaaggcc gatcgtgact tcttccccct
     5221 gaccgtcgat taccgcgaga agatgtacgc ggct
//
LOCUS       DASVOD010000081         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_996897, whole genome shotgun sequence.
ACCESSION   DASVOD010000081 DASVOD010000000
VERSION     DASVOD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996897"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF720_01620"
     CDS             complement(<1..424)
                     /locus_tag="VF720_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878075.1"
                     /translation="MMRLALRASAMRRITASRVPALVGGLACLGLAAFAATAPAQVTT
                     PVPQAQRGNSDNERSGFHDAGNIRTVFWNYGMVGDYPADPGGVDLSVFHSVEVPKGGG
                     MNYSDGITPFVLAKIRQRDGTESYIMETGFRERQGSSPF"
     gene            complement(421..>3074)
                     /locus_tag="VF720_01625"
     CDS             complement(421..>3074)
                     /locus_tag="VF720_01625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7878076.1"
                     /translation="IAFVAAWLAIIAATPAISGTTGKISGQVLNSDKQPVVAATVLVV
                     GQKLGAVTDAAGKYNILNVPAGTYDLSVGRLGFEKQLLTGLQVRADETVFQDFTLGTA
                     AVEMKEVVITAERPTVDVNLTSTKQTLTTEEIEDLPVQDLEDVVNLQAGVVDGHFRGG
                     RSGEVQYQVDGVSINNAYDNQPTLIIDRSLLQEVQVLSGTFDAEYGQAMSGVVNAVLK
                     DGTPEFEWAAEIYGGSWAFSNADDRRLTTDVSNPTAIQSYQLNLSGPLLGKETVFLVS
                     GRHYHYDDYITAERRFTPTDSSDFAGNVFTPTGDGGDEPLGYSREWSGVAKLTSTAVH
                     NARINYQALFNDIEGRRTNFAWRYNPDGASLQTTRSINHGLDWTQTLSSSTFLDATFR
                     HSYLHYTDHAYDDLYDPRYDDAGPGITDDAYEEGANIQGVDFTRFEQKTNAFLLKSAV
                     VSQLSAEHQVKVGGEIQWPEVSFGNPGYLTYTTVNGVQTLVRHDNEPPDFPGVVTYNP
                     VIAAAHVQEAAELRNLTVRSGLRLDYFDANSFIPGDLANPANAIEGVPTAPPKATTTK
                     VVLAPRLGVAFPVGQSAALHFAYGHFYQFPAIGTVFSNADYSVLSNLQVGDVSYGVLG
                     NPDVKPEKTIQYEFGYKHALSLDMGIDGTLFYKDVRDLLGAEFVSTYNGAEYARLTNT
                     DFGDVLGFTLAFDHRHLGPLVLGLDYTWQQAIGNSSDPRETATRASAGEDPRPRLVSF
                     NWDQRHTLNLTLAYRDTRGFSSSFVLRAGSGQPYTPVIESGFGNGLETNSGRKPTATQ
                     IDLRAEKGTSFQNFNVGLYGRVFNLLDSRFFNGAVFNSTGSPYYSRFPEADEVALNDP
                     SRFYQPRRIEFGLKFSPKQDDTTEKAP"
BASE COUNT          466 a          920 c         1120 g          568 t
ORIGIN      
        1 agaaggggga ggagccctgc cgctcgcgga agccggtttc catgatgtag ctttcggtgc
       61 cgtcgcgctg cctgatcttg gccagcacga acggcgtgat gccgtccgag tagttcatgc
      121 cgccgccctt cggcacttcc acggagtgga agaccgagag gtccacaccg ccgggatcgg
      181 ccgggtagtc gccgaccatg ccgtaattcc agaagaccgt gcggatgttg ccggcgtcgt
      241 ggaagccgga acgctcgttg tcgctgttgc cacgctgggc ctggggcacg ggcgtggtta
      301 cctgggcagg cgcggtggcg gcgaacgccg cgaggccgag gcaggcgagc ccgccgacga
      361 gcgccgggac gcgggacgct gtgatgcggc gcatggccga ggcgcgaagg gcgagccgca
      421 tcatggcgcc ttctccgtcg tgtcgtcctg tttcgggctg aacttcaggc cgaattcgat
      481 ccgacggggc tgatagaaac ggctggggtc gttgagggcc acttcgtcgg cctccggaaa
      541 acgggagtag taggggctgc cggtgctgtt gaagacggcc ccgttgaaga atcgcgagtc
      601 gagcaggttg aacacgcggc cgtagaggcc gacgttgaag ttctggaacg acgttccctt
      661 ctcggcccgc aggtcgatct gcgtggcggt cggcttccgt cccgagttgg tctcgaggcc
      721 gttgccgaag cccgactcga tgaccggcgt atagggctgg cccgaaccgg cgcgaaggac
      781 gaaggacgat gagaatcccc gcgtgtcgcg ataggcgagg gtgaggttca acgtgtggcg
      841 ctggtcccag ttgaacgaca ccaggcgggg gcggggatcc tcgccggcgg aggcgcgcgt
      901 ggcggtctct cgggggtcgc tggaattgcc gatggcctgc tgccaggtgt agtcgagccc
      961 gagcaccagg ggcccgaggt gacggtggtc gaacgccagc gtgaagccga gcacgtcgcc
     1021 gaagtcggta ttggtgaggc gggcgtactc cgcgccgttg tacgtggaca cgaattcggc
     1081 gccgagcagg tcgcgcacgt ccttgtagaa gagcgtgccg tcgatcccca tgtcgaggct
     1141 cagggcgtgc ttgtagccga actcgtactg gatcgtcttc tcgggcttga cgtccgggtt
     1201 gccgagcacg ccgtagctca cgtctcccac ctgcaggttc gagaggacgc tgtagtcggc
     1261 gttgctgaac accgtgccga tggccggaaa ctggtagaag tgaccgtagg cgaagtgaag
     1321 cgccgccgat tgccccaccg ggaaggccac gccgaggcgc ggggcaagca cgaccttggt
     1381 ggtggtggcc ttgggggggg cggtgggcac cccctcgatg gcgttggcgg gattggccag
     1441 gtcgcccggg atgaacgagt tggcgtcgaa gtagtcgagc cggagccccg accgcaccgt
     1501 caggttgcgc agctcggcgg cttcctgcac gtgggcggcc gcgatcaccg ggttgtaggt
     1561 aaccacgccc gggaagtcgg gcggctcgtt gtcgtgccgg accagggtct gcacgccgtt
     1621 caccgtggtg taggtcaggt agcccgggtt gccgaagctc acttcgggcc actggatttc
     1681 gccccccacc ttgacctggt gctcggccga gagctggctc accacggcgc tcttgaggag
     1741 gaaggcgttg gtcttctgtt cgaaacgggt gaagtccacc ccctggatgt tggcgccctc
     1801 ttcgtaggcg tcgtcggtga ttccaggccc ggcgtcgtca taccgtgggt cgtacaggtc
     1861 gtcgtaggcg tggtcggtgt aatgcaggta gctgtggcgg aacgtggcat ccaggaaggt
     1921 ggagctggag agcgtctgcg tccagtcgag cccgtgattg atcgaccggg tggtctggag
     1981 gctggccccg tccgggttgt agcgccaggc gaaattcgtc cggcgcccct cgatgtcatt
     2041 gaagagcgcc tgatagttga tgcgggcgtt gtgcacggcc gtgctcgtga gcttggccac
     2101 gcccgaccac tcgcgtgagt agccgagcgg ctcgtcgccg ccgtcacccg tgggggtgaa
     2161 gacgtttccg gcaaagtccg aggagtcggt cggtgtgaag cgccgctcgg cggtaatgta
     2221 gtcgtcgtag tgataatgcc ggccgctgac gagaaacacc gtctccttgc cgaggagcgg
     2281 tccgctcagg ttcagctggt agctctggat ggccgtgggg ttgctcacgt cggtggtaag
     2341 gcggcggtcg tccgcgttgg agaaggccca gcttccacca tagatctcgg cggcccactc
     2401 gaactccggc gtgccgtcct tcagcacggc gttcaccacg ccgctcatgg cctggccgta
     2461 ttccgcgtcg aacgtgccgc tcaacacctg cacttcctgg agcagcgagc ggtcgatgat
     2521 cagggtgggc tggttgtcgt aggcgttgtt gatgctcacg ccgtccacct ggtactgcac
     2581 ttcgccgctc cggccgccgc ggaaatgccc gtcgacgacg ccggcctgca gattgacgac
     2641 gtcctcgagg tcctggaccg gcaggtcttc gatctcctcg gtggtgagcg tctgcttggt
     2701 gctggtgagg ttgacgtcca cggtggggcg ctccgccgtg atgacgactt ccttcatctc
     2761 gacggccgcg gtccccagcg tgaagtcctg gaagaccgtt tcgtcggccc gcacctggag
     2821 gccggtgagg agctgcttct cgaagccgag tcgaccgacc gacaggtcgt acgtgccggc
     2881 cggcacgttc aggatgttgt acttgccggc cgcgtcggtg acggcgccca gcttctgccc
     2941 cacgacgagc acggtggcgg ccaccaccgg ctgcttgtcg gaattgagca cctgtcccga
     3001 tatcttgccg gtcgtgccgg agatggcggg cgtggcggcg atgatcgcca gccacgccgc
     3061 cacgaaggcg atga
//
LOCUS       DASVOD010000082         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_57336, whole genome shotgun sequence.
ACCESSION   DASVOD010000082 DASVOD010000000
VERSION     DASVOD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57336"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1248
                     /locus_tag="VF720_01630"
     CDS             <1..1248
                     /locus_tag="VF720_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878077.1"
                     /translation="PDATIQAMIAGPDGQLLVGTGGEKSGLYRVDPISRDWSLLARTT
                     APLILSLARTGNTLYIATGSPGRVYRAGLAGSPTGKLLSIVHDAKQTSDWGVVSWDDD
                     AADGRGISIRTRSGNTEKPDATWSAWSAPLSDARGSSITSPPARYIQWEAVLTGNAAL
                     SAVTVSWAEKNLPPLVSRITVSESGGTLQRGGDNGGPQPVVQALPGNVRAEFSVSTQG
                     SRRSASDEESSWARRYRTIRWEASDPNDDGLEYRLQYRARGETAWQLLDEELTEPIYV
                     LDTTRLPDGAFAVRVSVSDRPDNSPGEEKTAQRESEFFLVDNAPPVVEGLTATAAGDS
                     LDVGARVADASGSIKSAEISLDGEEWRRLLPLDRVWDEGSEEIRVTIARPSKDAGDLL
                     IRAFDDTGNKGVGRQPFPKKEKQ"
     gene            1245..>2272
                     /locus_tag="VF720_01635"
     CDS             1245..>2272
                     /locus_tag="VF720_01635"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX7878078.1"
                     /translation="MTFRHAADGVNVAVVGATGAVGEEMRRLLLERRFPIRQLRFFTS
                     PRSAGTAMPFGDRTVTAELLAEGCFDGVQLALFSAGATVSREWGPKAVAAGALVVDNS
                     SAWRMDPTIALIVPEINAHAIPAPPALIANPNCSTIQMVVALKPLHERFGLERVIVAT
                     YQSTSGGGRQAMNELDTATLGFQSGNEPAPVKFPHPIAFNLVPQVDEFDRLDYTKEEW
                     KMVNETRKIMEIPALPVTATCVRVPVHRGHSEVVWARFASPVNVAEARGALSAFPGVV
                     LEDDPAGRRYPTPRSAAGKDPVFVGRLRQDPTDDHALVFWVVSDNLLKGAALNAVQIA
                     EAACAAVGA"
BASE COUNT          352 a          782 c          795 g          343 t
ORIGIN      
        1 cccgacgcca cgatccaggc catgatcgcc gggcccgacg gccagctgtt ggtcggaacg
       61 ggcggcgaga agagcggcct ctaccgggtc gatcccatct cgcgtgactg gagccttctc
      121 gcgcggacga ccgcgccgct catcctgtcg ctggcgcgga cgggaaacac gctctacatc
      181 gccaccggat cgcccggccg tgtgtaccgt gccggccttg ccggaagtcc caccggcaag
      241 ctcctctcga tcgtgcacga cgcgaagcag acctcggact ggggtgtggt gagctgggat
      301 gacgacgcgg ccgacgggcg cggcatctcc atccggacgc ggagcggcaa taccgagaag
      361 cccgatgcca cctggagcgc ctggtcggca cctctttccg acgcgcgcgg ctcgagcatc
      421 acgagcccgc cggcccgcta catccagtgg gaagccgtgc tcaccggcaa cgccgcgctc
      481 tcggcggtca ccgtgtcgtg ggccgagaag aacctcccgc ccctggtgtc ccgcatcacc
      541 gtgtcggagt ccggcggcac gctgcagcgc ggcggcgaca acggcggccc gcagccggtc
      601 gtgcaggcgc tgccgggaaa tgtgcgcgcc gaattttcgg tgtcgacaca ggggagccgg
      661 cgatcggcca gcgacgagga atcgagctgg gcgcgccgct atcgcacgat ccgctgggaa
      721 gccagcgacc cgaacgacga cggcctcgaa tatcgactgc agtatcgggc ccggggcgaa
      781 accgcctggc agctcctcga cgaggagttg accgagccga tctacgtgct cgacacgacg
      841 cgccttcccg atggcgcctt tgcggtccgg gtgtcggttt ccgaccgacc ggacaactcg
      901 ccgggcgagg agaagacggc ccagcgcgaa tccgagttct ttctggtcga caacgcgccg
      961 cccgtcgtgg aaggtctgac ggccacggcg gccggcgatt cgctcgacgt gggagcgcgc
     1021 gtggccgacg cgtccggttc gatcaagtcg gccgagatct cgctcgacgg cgaggagtgg
     1081 cgccgcttgc tgccgctcga ccgcgtgtgg gacgaggggt cggaggagat ccgcgtgacg
     1141 atcgcccggc cctcgaagga cgcgggcgac ctgctgatcc gcgccttcga cgataccggc
     1201 aacaagggcg ttggacggca gccgttcccg aaaaaggaga agcagtgacg tttcgccacg
     1261 cggccgacgg cgtgaacgtg gcggtggtcg gggccaccgg cgcggtgggg gaggagatgc
     1321 gccgtctcct cctcgagcgg cgcttcccca tccgccagct tcgttttttc acctcgcccc
     1381 gttcggcggg aaccgcgatg ccgttcggtg atcgcaccgt gacggccgag ttgctggccg
     1441 aggggtgctt cgacggcgtg cagctcgccc tcttctcggc gggcgccacg gtgagccgcg
     1501 agtgggggcc gaaggccgtg gccgccggcg ccctggtggt cgacaattcc agcgcctggc
     1561 gcatggaccc gaccatcgcc ctcatcgtgc ccgagatcaa cgcgcatgcc attccggcgc
     1621 cgccggcgct catcgccaat ccgaactgtt ccacgatcca gatggtggtg gcgctcaagc
     1681 cgctgcacga gcgtttcggc cttgagcggg tgatcgtggc cacgtaccag tccacttcgg
     1741 gcgggggtcg ccaggccatg aacgaattgg atacggccac gctcggcttc cagtcaggca
     1801 acgaacccgc gccggtcaag tttccgcacc cgatcgcctt caacctcgtg ccgcaggtcg
     1861 atgagttcga ccgcctcgat tacacgaagg aagaatggaa gatggtgaac gagacgcgga
     1921 agatcatgga gatccccgcg cttcccgtca ccgccacctg tgtgcgcgtg ccggtgcatc
     1981 gcggccactc ggaggtggtg tgggcccggt tcgcatcgcc cgtgaatgtg gccgaggcac
     2041 ggggcgcgct ctccgcgttc cccggtgtcg tgctcgaaga cgatccggcg ggcaggcgtt
     2101 atcccacgcc gcggagcgcg gcggggaagg acccggtctt cgtggggcgc ctccggcagg
     2161 acccgaccga cgaccacgcg ctcgtcttct gggtcgtgtc ggacaacctc ctcaaggggg
     2221 ccgcgctcaa cgccgtccag atcgccgagg ccgcctgcgc ggccgtcgga gc
//
LOCUS       DASVOD010000083         5060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_423008, whole genome shotgun sequence.
ACCESSION   DASVOD010000083 DASVOD010000000
VERSION     DASVOD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423008"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..601)
                     /locus_tag="VF720_01640"
     CDS             complement(110..601)
                     /locus_tag="VF720_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX7878079.1"
                     /translation="MSTRTLALASAAAGLVSWVSGALFAVSVSLWQAELGEALPTVLL
                     LTCLAAGLLMMVFLAGYVYSDAESRGMRGALWALLIFFFASLPGFLVYLLMRTPKLRH
                     CPNCHTPVKDDYVVCPNCEAPVARACPGCGRRADPAFRVCPYCQHDLTGAARLERRGG
                     TTA"
     gene            complement(598..1140)
                     /locus_tag="VF720_01645"
     CDS             complement(598..1140)
                     /locus_tag="VF720_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878080.1"
                     /translation="MNWNPFAHRPGACETVQDRLLEKAGTDPGHGSLEADLARHLAGC
                     VTCRELADGLALTSRLHPHWKSEMPPPDLALKTIEFLEPHWKRTEKPVNREEIRLIWT
                     GALGMAGATAALLLFVADSGNAVGHAIAAEGILRFGARLTLLQLAGGTIVSVALLVIR
                     AARSSDSDARPRERNRGGNE"
     gene            complement(1137..1709)
                     /locus_tag="VF720_01650"
     CDS             complement(1137..1709)
                     /locus_tag="VF720_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7878081.1"
                     /translation="MARAQTGDRAAFAELVFRYEKPLYGYLRRLLRHDEAAEDTFQET
                     WYRILKAVDRFNPEFRFAPWLYRIATNLCRDAARRGKIRNHRSLDAPMSGESDTTLGE
                     MVPGNELAPDVMTEGSDVGTQVARFVGELPDKQREAFVLRHYQGLTYEEIARAQRCSL
                     AAVKSNIHHAVVSLRRSLDRIGLSPAASSS"
     gene            complement(1800..3362)
                     /locus_tag="VF720_01655"
     CDS             complement(1800..3362)
                     /locus_tag="VF720_01655"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEX7878082.1"
                     /translation="MTTPTGRDDRLDELRRRKEAAREGGGTERVAKMHAAGKLTAHER
                     IDLLLDRGTFTELGVFVTHRSTDFGIGDKKITGDGVVSGFGRIDGRLVYVFAQDFTVF
                     GGTLSESNARKICQIMDMALENGAPVIGLNDSGGARIQEGVQSLGGYAEIFLRNTLAS
                     GVVPQISAIMGPCAGGAVYSPAITDFTIMTKETSQMFVTGPDVIKMVTREEVTHEELG
                     GAMTHNSKSGVAHFAAEDDRHCLMMIRQLLSFLPSNNTDDAPRKDTGDDLNRMDERLK
                     TIVPPEPNKPYDMKDVVRCVVDNGDFLEVHEHFAPNIVVGFARLNGRPVGIVGNQPKH
                     LAGVLDIKSSVKGARFVRFCDAFNIPLVTFEDVPGFLPGTAQEWNAIITNGAKLLYAY
                     CEATVPKLTVITRKAYGGAYDVMSSKHIRGDYNVAWPAAELAVMGAEGAVNIIFRGEL
                     AKAKDPDAERKRLVADYNTKFANPYVAASLGYLDDVIEPQETRPKLIAALEMLQNKRQ
                     SLPPKKHGNIPL"
     gene            3451..4191
                     /locus_tag="VF720_01660"
     CDS             3451..4191
                     /locus_tag="VF720_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7878083.1"
                     /translation="MERHIARLQWKEFQRLVPEKCITALLPIGILEAHGAAALGTDIL
                     IPERMSELLAEPVGALIAPTIPYGVPGSLFGYPGTIGVREDIFADYVADVLHSLASAG
                     FRQVFILNGHGGNNEALKEVARESWQAGGLAVAVVHWWMECGDIVKEVYGGAGGHGGV
                     DETALMLAISSDLVEKRPRQVDTPFRMRPSVVTYPAPSSLLVFEEGAAPVSFDEKKAR
                     QFEKRVAARLEEIIEEIEARWAETGFTS"
     gene            4188..>5060
                     /locus_tag="VF720_01665"
     CDS             4188..>5060
                     /locus_tag="VF720_01665"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7878084.1"
                     /translation="MRAGTSTPGHRVAIVAAVRTPIGKFLGSLSEVSAVDLAVAAAKG
                     AIARSGLDPSYFGETILGQARQAGSAPNPGRQVSVKAGLSFEVPASTINQACASGLRA
                     IQLGVQSIRAGEAEAVLAGGVESMSRVPFLLEGLRKGWKMGHQPLVDNMYRDGFTCGL
                     CGKIMGETAETLATQYAIPREQQDAYAVRSQNRAEAARKAGRFTAEIIPVDVPGRGGP
                     TRVEIDEHIRDGATPADLAKLPAVFSKTGTVTAGNASGITDGAAMLVLASEALVRRKN
                     LEVLAWYESGTLVGV"
BASE COUNT          789 a         1666 c         1758 g          847 t
ORIGIN      
        1 ggccagcccg atacccggca acaggagggc aagcgccgcc aggacgacgc ggccgatatc
       61 cgggaggctg acgggcatcc ggtattgata cccgcccgcc agtggtgaat caagccgttg
      121 tgccgccccg ccgttcgagt cgcgccgcgc cggtcaggtc gtgctggcag taggggcaca
      181 cgcggaatgc cggatcggcg cgcctcccgc aaccggggca ggccctcgcc accggcgcct
      241 cgcagttggg gcacaccacg tagtcgtcct tcaccggcgt atggcagttg gggcaatggc
      301 gcagcttggg cgtgcgcatc aggaggtaca ccaggaaccc cggcaggctg gcgaagaaga
      361 agatgaggag cgcccagagc gccccgcgca tgccgcgcga ttcggcgtcg gagtaaacgt
      421 aaccggccag gaagaccatc atcaggaggc cggcggccag gcaggtgagg agcagcacgg
      481 tggggagggc ctccccgagt tcggcctgcc acagcgacac gctgacggcg aagagtgccc
      541 ccgacaccca ggaaacaagg ccggccgcgg ccgacgccag cgcgagcgtg cgggtgctca
      601 ttcgttccct ccgcggttgc gctcgcgcgg tcgggcatcg gagtcggacg agcgcgcggc
      661 ccggatcacc aggagcgcca ccgacacgat cgtaccgccg gccagctgga gcagggtgag
      721 ccgggcgccg aaccggagga tcccctcggc ggcgatggca tgccccaccg cgttgccgct
      781 gtcggccacg aagaggagga gcgccgcggt ggcgccggcc atgccgagcg cccccgtcca
      841 gatgaggcgg atctcctcgc gattgaccgg cttttcggtc cgtttccagt ggggctcgag
      901 gaattcgatc gtcttcaagg cgaggtcggg tggtggcatt tcggacttcc agtgcggatg
      961 gagtcgcgag gtgagcgcca gcccatcggc aagctcgcgg caggtgacgc aaccggccag
     1021 gtggcgcgcg agatcggcct ccagggagcc gtgccccgga tcggtgccgg ctttctccag
     1081 cagtcgatcc tggacagttt cgcaagcgcc cggtcgatgg gcgaagggat tccagttcat
     1141 gacgagctcg cggcggggga aaggccaatc cggtcgagcg agcgtcggag cgacaccacc
     1201 gcgtggtgaa tgttcgattt caccgcggcc agcgagcaac gctgggcgcg ggcgatttcc
     1261 tcgtaggtca gtccctggta gtgacgcagg acgaacgcct cacgctgctt gtcggggagc
     1321 tcgccaacga accgcgccac ctgcgtcccc acgtcgctcc cctcggtcat gacgtcgggg
     1381 gccagttcat tgccaggcac catctccccg agcgtcgtat cggattcacc cgacatcggc
     1441 gcatccagag agcggtgatt ccggatcttt ccccggcggg ccgcatcccg gcagagattg
     1501 gtggcgatcc gatacagcca gggggcgaaa cggaactcgg ggttgaagcg gtccaccgcc
     1561 ttcaggatgc gataccaggt ttcctggaag gtgtcctcgg cggcttcatc gtgacgcagc
     1621 agtcgccgga ggtagccgta aagcggcttc tcataacgaa atacgagttc ggcgaaagcg
     1681 gcccgatcgc cggtttgtgc ccgggccatc agctcctcgt ccttgatttc ggggtagcgg
     1741 ggggcttcca cagcgggaga ttgtacgccc cgacccgggg aaggtcgcgg aggaattctc
     1801 tacagcggga tattcccgtg cttcttcggg gggaggctct gccgtttgtt ctggagcatt
     1861 tccagggcgg cgatcagctt cgggcgggtt tcctggggct cgatgacgtc gtccaggtag
     1921 ccgaggctgg cggccacgta ggggttggcg aacttggtgt tgtagtcggc cacgaggcgc
     1981 ttgcgctcgg cgtcggggtc cttggccttg gccagttcgc cgcggaagat gatgttgacg
     2041 gccccctcgg cgcccatcac ggccagctcg gcggccggcc aggccacgtt gtagtcgccg
     2101 cgaatgtgct tgctgctcat gacgtcgtag gccccgccgt aggccttgcg ggtgatgacc
     2161 gtgagcttgg gcaccgtggc ctcgcagtag gcgtacagga gcttggcgcc gttggtgatg
     2221 atggcgttcc actcctgcgc cgtgcccggc aggaagccgg gcacgtcctc gaacgtcacc
     2281 agcggaatgt tgaacgcgtc gcagaagcgg acgaaccgcg cccccttcac gctcgacttg
     2341 atgtcgagca cgccggccag gtgcttcggc tggttgccga cgatgccgac cggccggccg
     2401 ttcaggcgcg cgaaccccac cacgatgttg ggcgcgaagt gctcgtgcac ctcgaggaag
     2461 tcgccgttgt ccaccacgca gcggaccacg tctttcatgt cgtagggctt gttgggctcg
     2521 ggggggacga tcgtcttcag acgttcatcc atccggttca ggtcgtcgcc tgtgtccttc
     2581 cgcggcgcgt cgtcggtgtt gttgctgggc aggaacgaca ggagctgccg gatcatcatc
     2641 aggcagtggc ggtcgtcctc ggctgcgaag tgggccacgc cgctcttgga gttgtgcgtc
     2701 atggccccgc ccagctcctc gtgcgtcact tcctcgcgcg tcaccatctt gatgacgtcc
     2761 gggccggtga cgaacatctg gctggtttcc ttcgtcatga tcgtgaagtc ggtgatggcc
     2821 gggctgtaca ccgccccgcc ggcacacggc cccatgatgg ccgagatctg cggcacgacg
     2881 ccggaggcca gcgtgttgcg gaggaagatc tcggcgtagc cgccgaggct ctggacccct
     2941 tcctggatgc gggcgccgcc cgagtcgttg agcccgatga ccggggcgcc gttctcgagc
     3001 gccatgtcca tgatctggca gatcttccgc gcgttcgatt cggagagcgt gccgccgaac
     3061 acggtgaagt cctgggcgaa cacgtacacc agccgcccgt cgatccggcc gaagcccgaa
     3121 acgacgccgt cgccggtgat cttcttgtca ccgatgccga agtcggtcga ccggtgagtg
     3181 acgaagacgc cgagctcggt gaaggttccc cggtcgagca gcaggtcgat ccgctcgtgg
     3241 gccgtgagct tgccggcggc gtgcattttc gccacccgct ccgtgccccc cccttcgcgg
     3301 gcggcttcct tgcggcggcg gagttcgtcg agccggtcgt ccctgccagt cggtgtcgtc
     3361 atgggaggcg gagggtaatg agcggggggc aaccggtcaa cgccgcccgg cttgtcaccc
     3421 gcccccctcc cgggtaggct ttccggcctc atggaacgac acatcgcacg cctgcagtgg
     3481 aaggaattcc agcgcctcgt ccccgagaag tgcattacgg cgctcctccc gatcggcatc
     3541 ctcgaggccc acggcgccgc ggccctcggt accgacatcc tgattccgga gcggatgtcc
     3601 gagctcctgg ccgaaccggt cggtgcgctc attgccccta ccattcccta cggcgtgccg
     3661 gggagcctct tcggttatcc cggcaccatc ggcgtccgcg aggacatctt cgccgactac
     3721 gtggccgacg tcctccattc gctggcctcg gcaggcttcc ggcaggtctt catcctgaac
     3781 ggtcacggcg gcaacaacga ggccctgaag gaagtggccc gcgagtcctg gcaggccggt
     3841 ggcctggccg tggccgtggt ccactggtgg atggagtgcg gcgacatcgt gaaggaagtg
     3901 tacggcggcg ccggcggcca tggcggcgtg gatgaaaccg cgctgatgct ggccatctcc
     3961 tccgacctcg tcgagaagcg cccgcggcag gtcgatacgc cctttcgcat gcgtccgtcg
     4021 gtcgtcacct atccggctcc ttccagcctg ctcgtcttcg aggagggggc cgcgccggtt
     4081 tccttcgacg agaagaaggc cagacagttc gagaagaggg tggccgcccg tctcgaggag
     4141 atcatcgagg agatcgaggc ccgctgggcg gagacgggat tcacgtcgtg agggccggca
     4201 cgtcgacacc cggtcaccgc gtggccatcg tggccgccgt ccgtacgccg atcggaaaat
     4261 tccttggctc actctccgag gtgagcgccg ttgacctggc ggtggcggcg gcaaagggcg
     4321 ccatcgcccg atcggggctc gacccgtcct atttcggtga aacgatcctt ggccaggctc
     4381 gccaggccgg aagcgcccca aaccccggcc ggcaggtttc ggtcaaggcc ggcctctctt
     4441 tcgaggtgcc ggcctccacc atcaaccagg cctgcgcctc cggccttcgc gccatccagc
     4501 tgggcgtgca gtcgatccgg gccggcgagg ccgaggcggt gctggccggc ggcgtggaat
     4561 cgatgagccg ggtcccgttc ctcctcgagg gtctccgcaa ggggtggaag atgggtcacc
     4621 agccgctggt cgacaacatg taccgggacg gcttcacctg cggtttgtgc ggaaagatca
     4681 tgggggagac ggccgagacc ctcgccacgc agtacgccat cccgcgcgag caacaggacg
     4741 cctatgccgt ccgcagccag aaccgggcgg aagccgcgag gaaggcgggc cggttcaccg
     4801 ccgagatcat tccggtggac gtgccaggac gtggtggccc gacgcgggtc gaaatcgacg
     4861 agcacatccg tgacggcgcc acacccgccg acctggcgaa gctcccggcc gtcttctcta
     4921 agaccggcac cgtcacggcc ggcaacgcct cgggaatcac cgacggcgcc gccatgctcg
     4981 tgctggcctc cgaggcgctg gtccggcgga agaatctcga ggtcctggcc tggtacgagt
     5041 ccggcaccct ggtgggcgtc
//
LOCUS       DASVOD010000084         2575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_266240, whole genome shotgun sequence.
ACCESSION   DASVOD010000084 DASVOD010000000
VERSION     DASVOD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266240"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VF720_01670"
     CDS             <1..1415
                     /locus_tag="VF720_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7878085.1"
                     /translation="FVVTWTVGSPARTRLPLDGAIMGLASTRNGDVRLDVVTLANGHV
                     AVLADGEFAPGWPRPTGGLLFGDPVLLELGGGAEPEIAVAGSDSLMYVWSLDGTSRPG
                     FPARLPDAVLTSSSAGDLDGDGIPELVVVTADDRVSIVRMGGSVRNWSDANPAPLAGA
                     SHAPPILADVTGDGRLDVVLTGNDRVMVLGNPAGPSVTWMSTWPMSDPLVGEVIAADL
                     TGVGSFAVVTTHASGKVVVRPYGGLNGSPIAEIETDSEPAASALAIPIDGNGETGLAV
                     VTVDGCVRAFRATGEAVSGWPKRLAADAGGRWAAGSDPGRDAPVLNVEAVDGTLQRFA
                     LAAAGPVVSFAWGMVGANPARTRSVAPTTPAAPVDPERPDVVRLPPGLLALPNPARAG
                     APVTFAPAGGRTVMSLVIHDVLGRVVRRIDAATNDNRSGVLSWDGRSDVGRPPAAGFY
                     VARARLDDGTVATARFVVLR"
     gene            complement(1424..1834)
                     /locus_tag="VF720_01675"
     CDS             complement(1424..1834)
                     /locus_tag="VF720_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7878086.1"
                     /translation="MTRSLFAGILAVGLVAMAGCGSDSSSTGPDTAPPSAPQLLSGYA
                     KTEGKVVLTWAPNVESDLDGYNIYEVGVDEPIAVAPAGSRGVAIETTVTGEIPFRITA
                     IDRSGNESAPSSTLSIGYHPLPTPEPDNVIELRG"
     gene            2133..>2575
                     /locus_tag="VF720_01680"
     CDS             2133..>2575
                     /locus_tag="VF720_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family peptidase"
                     /protein_id="HEX7878087.1"
                     /translation="MRAARRGVVLGLLLLVPTGVRGADRGYHAPIAATCPVSSSFGEY
                     RANHFHGGLDFSTSGEVGLPIHAIADGWVWRVRASGSGYGRALYVRLADGRTQVTAHQ
                     SRFAPAIAAFVAAAQESLDRYEVDLSPGEGRLPVKRGEIIAWSGES"
BASE COUNT          350 a          866 c          932 g          427 t
ORIGIN      
        1 gtttcgtggt gacgtggacg gtgggctcgc cggcgcgaac ccggctgccg ctggacggcg
       61 ccatcatggg tcttgcctcc acccggaatg gtgatgtgcg cctcgatgtc gtcaccctgg
      121 ccaacggcca cgtggcggtc ctcgccgatg gagagttcgc gcccggctgg ccgcgtccga
      181 cgggtggcct cctcttcgga gacccggtgc ttctcgagct cggcggggga gccgaacccg
      241 agatcgccgt ggccggcagc gacagcctca tgtacgtctg gtcgctcgac ggaaccagcc
      301 ggcccggctt tccggcgcgc ctccccgacg ccgtgctcac aagttcctcc gccggcgatc
      361 tcgacggtga cggcatcccc gagctggtgg tggtgacggc cgacgaccgc gtctcgatcg
      421 tccggatggg aggttcggtt cgcaactggt ccgatgcgaa cccggcacca ctcgccggcg
      481 catcgcacgc gccgccgatc ctggccgacg tgacaggcga tggacgactg gacgttgtcc
      541 tgactgggaa tgaccgggtg atggtcctcg ggaatccggc cggcccgagc gtgacctgga
      601 tgtcgacatg gccaatgagc gatcccctcg tcggcgaagt catcgcggcc gacctcacgg
      661 gtgtcgggtc atttgccgtc gtcacgactc acgcatccgg aaaggtggtc gtgaggccat
      721 acgggggatt gaacgggagt ccgatcgcgg aaattgagac ggactccgag ccggcggcct
      781 ccgccctcgc cattcccatc gacgggaacg gtgagaccgg actcgccgtc gtcacggtcg
      841 acggctgcgt gcgcgccttc cgcgccaccg gcgaagccgt ttccggatgg ccgaagcgcc
      901 ttgcggctga tgccggcggc cggtgggccg ccggttccga cccggggcgc gacgccccgg
      961 tgctgaacgt ggaggccgtg gacgggacgc tccagcgctt cgccctggcg gcggcgggtc
     1021 cggtggtttc gttcgcctgg gggatggtgg gcgccaaccc cgcacggacg cgaagcgtgg
     1081 cgccgacgac tccggccgca ccggttgatc ccgaacgtcc cgatgtcgtg aggcttccgc
     1141 cggggcttct cgccctcccg aaccccgcgc gcgccggtgc gccggtcacg ttcgcgccgg
     1201 cgggtggccg aacggtcatg agcctcgtca tccacgacgt cctcggtcgc gtggttcgcc
     1261 ggatcgatgc cgccaccaac gacaatcgct ccggcgtcct gtcatgggac ggtcggagcg
     1321 atgtcggtcg gcctcctgcg gccgggttct acgtggcacg ggcccggctc gacgacggga
     1381 cagtggcgac tgcccggttc gtcgtcctcc ggtgacgcgg ctctcagccg cgcagttcga
     1441 tcacgttgtc cggttcgggc gtggggagcg ggtggtagcc gatcgacagc gtgctggacg
     1501 gggcgctctc gttgcccgaa cgatcgatgg cggtgatccg gaacgggatt tcgccggtca
     1561 ccgtcgtttc gatggccacg ccgcgactac cggcgggagc caccgcgatc ggctcgtcga
     1621 cacccacttc atagatgttg tagccgtcga gatcgctctc gacgttgggg gcccaggtca
     1681 ggacgacctt cccttccgtc ttggcgtatc ccgaaaggag ctggggcgcc gatggaggtg
     1741 ccgtgtcggg tccggtcgag gacgaatcgc tgccgcagcc ggccatggcg acgaggccca
     1801 cggccaggat gccggcgaaa agggaacggg tcatcggggt attcctcctt gcggtacggt
     1861 caacgcgatc acgcggtcga atcaaggctg tcggcggcgg cccgttcggg tcgacacccg
     1921 gcggtccgac aaggcaaccg ccgtgccatg gatcgggagg aggaacgtca aacttgcgcg
     1981 gaacccccaa gtggtggcga gctaagcgct tgaattgggt ctcttcaccc cgtttggagg
     2041 gggcggaagt tcgatggggg agcgcggagc ggcccatgcg gggcaatctg cacggggccg
     2101 acgtgcgccc tgcagcgcgg gattgtcgtc cgttgcgggc tgcacgccgg ggggtcgtgc
     2161 tcggactcct cctcctcgtg ccgaccggcg tgcgcggcgc cgatcgcggt taccacgcgc
     2221 cgatcgccgc tacctgcccc gtctcgagca gcttcggcga ataccgggcc aaccattttc
     2281 acggcggact cgacttctcg acctccggcg aggtcggcct tcccatccat gccatcgccg
     2341 acggctgggt ctggcgggtg cgcgcctcgg ggtccggcta tggccgggcg ctctacgtgc
     2401 ggctggccga cgggcgcacg caggtcacgg cccaccagag ccgcttcgcg ccggccatcg
     2461 ccgccttcgt ggccgcggcc caggaaagcc tggatcgtta cgaggtcgat ctatcgccgg
     2521 gcgagggccg cctgccggtg aagaggggtg agatcatcgc gtggagcggc gagtc
//
LOCUS       DASVOD010000085         2665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_631390, whole genome shotgun sequence.
ACCESSION   DASVOD010000085 DASVOD010000000
VERSION     DASVOD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631390"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..844
                     /gene="pstA"
                     /locus_tag="VF720_01685"
     CDS             8..844
                     /gene="pstA"
                     /locus_tag="VF720_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX7878088.1"
                     /translation="MVATVAVIVFEIVAGGAGVISWDFLTKPPSDGLRAGGIGPAIFG
                     TVALVLLMVIAVIPFGAMAAIYLHEYSRPGSRWTKAVRFAVSNLAGVPSIVFGLFGLG
                     FFIQFLGVGIDRAMGLDVTWGQPCMLWAALTLALLNLPVVIVTTEEALSSVPQHYREA
                     SLALGATRWQTIWRVVLPQALPGILTGGILAVGRGAGEVAPILFTGAAYFLPYLPTQL
                     TDQFMHLGYHTFVLATQSPDVDATRPVMFGTVLVLLALTLLLNLTATLIRARTRARQK
                     GG"
     gene            955..1722
                     /gene="pstB"
                     /locus_tag="VF720_01690"
     CDS             955..1722
                     /gene="pstB"
                     /locus_tag="VF720_01690"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018125938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX7878089.1"
                     /translation="MDARLPVKFTVTDLDFYYGATKVLKQVGLEIPEHRVTALIGPSG
                     CGKSTFLRTLNRMNDIIPGARAAGRVLLDGEDIFANGVDVVELRRRVGMVFQKSNPFP
                     KSIWDNVAYGLVVNGVSDRNFIAERVESSLMGAALWDEVKDRLKSSALGLSGGQQQRL
                     CIARALAVEPEVLLMDEPASALDPIATQKIEDLIDSLKERYTIVIVTHNMQQAARVSD
                     FTGFFYLGELIEVGPTEKIFTNPDKTSTEDYITGRFG"
     gene            1751..2437
                     /gene="phoU"
                     /locus_tag="VF720_01695"
     CDS             1751..2437
                     /gene="phoU"
                     /locus_tag="VF720_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX7878090.1"
                     /translation="MNTRQHSSREFEAELQSVKNALLAMGGRAEKSLGDAIRALVDRD
                     SELANRTIAADRDTDRAEMEIDELTLRILALRQPVGSDLRFLATALKIVTDLERVGDL
                     TVNICERVLELNNEPQLKPYIDLPRMAGRVSAIVRASLDAYVAGDVEKAHDVLASEQA
                     IDDLNVQIFRELVSFMVEDPRTITRAMRLIFIAKYLERISDHATNIAEQVIFMVKGSD
                     VRHGASIKGS"
     gene            2463..>2665
                     /locus_tag="VF720_01700"
     CDS             2463..>2665
                     /locus_tag="VF720_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878091.1"
                     /translation="MAVGFAIARPAGPAGAADWPKISDEERKLASVDPAGPGAVILFE
                     EAEVDDRAAQGRVQSLYRRFKILL"
BASE COUNT          452 a          905 c          851 g          457 t
ORIGIN      
        1 cctcctcatc gtggccacgg tggccgtcat cgttttcgag atcgtggccg gcggcgccgg
       61 cgtcatcagc tgggacttcc tgacgaagcc cccctcggac ggactccggg ccggcggcat
      121 cggcccggct atcttcggca cggtggcgct tgtgctcctc atggtcatcg ccgtcatccc
      181 gttcggcgcg atggccgcca tctatcttca cgagtattcg cgacccggct cgcgctggac
      241 gaaggccgtg cggttcgcgg tgagcaacct ggccggcgtg ccgtccatcg tcttcggatt
      301 gttcggcctt ggcttcttca tccagttcct cggcgtcggg atcgaccggg caatgggcct
      361 ggacgtgacc tggggccagc catgcatgct ctgggccgcg ctcacgctgg ccctcctcaa
      421 cctgccggtg gtgatcgtca cgaccgagga agcgctctcc tccgtgccgc agcattatcg
      481 cgaagccagc ctggccctgg gcgccacccg ctggcagacc atctggcgcg tggttctacc
      541 gcaggcgttg cccggcatcc tgaccggcgg catcctggca gtggggcgtg gcgcggggga
      601 agttgcgccc atcctcttca ccggggccgc gtatttcctt ccctatcttc ccacgcagct
      661 caccgatcag ttcatgcacc tcggctatca cacgtttgtc ctggccacgc agtcgccgga
      721 cgtggatgcc acacgtcccg tcatgttcgg aaccgtgctg gtcctcctgg cgttgacgct
      781 cctgttgaac ctgaccgcca cgctcatccg ggcccgcacg cgggcgcggc agaagggcgg
      841 atgacctttg aaaggagctg ccatcccatg agcaggatga cgacattgcc ggcgcccctg
      901 atatcctcgg cgatgaaaga ggcgccgcga ccggcgccgg aaggaggccc cgccgtggac
      961 gccaggctcc cggtcaagtt caccgtcacc gatctcgact tttactatgg agcgacaaaa
     1021 gtcctgaagc aggtgggcct cgagatcccc gagcatcggg tgaccgcgct catcggccct
     1081 tccgggtgcg gcaagtccac cttcctccgg acgctcaatc gaatgaacga catcattccc
     1141 ggggcgcggg ccgccgggcg ggtgctgctc gacggagaag acatcttcgc gaacggggtg
     1201 gacgtggtcg agctccgccg ccgcgtgggg atggtgttcc agaagtcgaa cccgtttccg
     1261 aaatcgatct gggacaacgt ggcctacggc ctcgtggtga acggcgtgag cgaccggaat
     1321 ttcatcgccg agcgggtcga gtcgagcctc atgggggcgg cgctgtggga cgaggtcaag
     1381 gatcgcctca agtcctcggc gctcggcctt tccggcggac agcagcagcg cctctgcatc
     1441 gcgcgggccc tggccgtgga gccggaggtg ctcctcatgg atgaaccggc ctcggcgctc
     1501 gatccgatcg ccacccagaa gatcgaggac ctgatcgaca gcctcaagga gcgctatacg
     1561 atcgtcatcg tcacccacaa catgcagcag gccgcccgcg tttccgactt caccggcttc
     1621 ttctaccttg gtgaactgat cgaggtgggg ccgaccgaga agatcttcac caatccggac
     1681 aagaccagca ccgaggatta catcaccggg cggttcggat aaaatggacg ggcacaagga
     1741 ggcgcccttc gtgaacacac gccagcattc cagccgcgaa ttcgaagccg agctccagtc
     1801 ggtcaagaac gccctcctgg ccatgggagg gcgcgccgag aagtcgctgg gcgacgccat
     1861 ccgcgccctg gtcgaccggg acagcgagct ggccaaccgg acgatcgccg ccgaccgcga
     1921 taccgaccgc gccgagatgg agatcgacga gctcacgctc cggatcctgg cgctccgcca
     1981 gccggtcggc tccgacctcc gcttcctggc caccgcgctc aagatcgtca ccgacctgga
     2041 gcgcgtcggc gacctcaccg tcaacatctg cgagcgggtg ctcgagctca acaacgagcc
     2101 gcaactcaag ccctacatcg acctgccgcg catggcagga agggtgagcg ccatcgtgcg
     2161 ggccagcctc gatgcctacg tggccggcga tgtcgaaaag gcccacgatg tccttgcgag
     2221 cgagcaggca atcgacgacc tcaacgtgca gatcttccgc gagctggtct cgttcatggt
     2281 ggaggatccg cggacgatca cccgggcgat gcgcctcatc tttatcgcca agtacctcga
     2341 gcggatcagc gatcacgcca cgaacatcgc cgagcaggtc atcttcatgg tcaaagggag
     2401 cgatgtccgt cacggcgcca gcatcaaggg ttcgtagagt catccggacg gccgcgtgcg
     2461 ttgtggccgt cggcttcgcg atcgcaaggc cggcgggccc ggccggggcg gccgactggc
     2521 cgaagatcag tgacgaggaa cggaagctcg cctcggtcga tccggccggt ccgggcgccg
     2581 tcatcctctt cgaggaagcc gaagtcgatg accgcgcggc ccaggggcgg gtccagagcc
     2641 tctatcgccg cttcaagatc ctcct
//
LOCUS       DASVOD010000086         5745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_527234, whole genome shotgun sequence.
ACCESSION   DASVOD010000086 DASVOD010000000
VERSION     DASVOD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527234"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VF720_01705"
     CDS             complement(<1..1416)
                     /locus_tag="VF720_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017365297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7878092.1"
                     /translation="MSLSGFHPAVSRWFTDRFGAPTAAQARGWAEIRRGRHTLIAAPT
                     GSGKTLAAFLSALDELFREGETGPLPDETRILYVSPLKALSADIHRNLAEPRREIRAL
                     AEAMGLPAARVTAAVRTGDTPSRERTAMLKTPPHILVTTPESLYLLLTAERSRRMLAT
                     VRTVIVDEIHAVIESRRGAHLALSLERLEHVTNGRLQRIGLSATQKPIDEVARYLVGG
                     GAVAGDGTPDCAIVDEGHRRALDLAIEVPGSPLEAVMSNDVWEEIYDRLAVLAFAHRT
                     TLVFVNTRRWCERVAHHLSRRLGEERVTAHHGSLSKEKRHDAETRLKEGSLSLLVATA
                     SLELGIDIGSVDLVVQLGSPGRISTFLQRVGRSGHTIHGTPKGRLFPLSREDLVECTA
                     LVRAVKAGELEHLRIQPAPLDVLAQQIVAEVAAEDWSVDDLYLALGRAWPYRRLDRAT
                     FDEVLEMTAHGFSTRRGRRGAL"
     gene            complement(1494..2765)
                     /locus_tag="VF720_01710"
     CDS             complement(1494..2765)
                     /locus_tag="VF720_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7878093.1"
                     /translation="MRKTNVFLPMILAALALANPVAALEPTTISFMIWGDPVEKQAYD
                     QLVSSFHAGHPDILVDLIHVPNQNLYIQRLTTDFVAGTPADVFLLNYRRMAPFAAKGR
                     IEPLDSRLKKSALIHEDDFYREAMEPFRWDGKQMAIPQNISSLVVYYNRSLFAAAGIP
                     YPKPDWTWDEFIATAKALTKDTDGDGTIDQYGLGTEAVVFRVFPFIWQNGGDFFDDPE
                     KPTRLTLDTPETRGALEWFIGFQSVHKIVPDFVAEKAEPSVHRFMRGAMAMYFDSRRG
                     VPVYRNVQGFDWDVAPLPTGKQPAGILHSDGYFMSKQSKHKDAAWTFIEYANSEEGQK
                     LMCGTGRTVPSMRRVAESQVFLETDHKPASSQVFLDVIPHIRAVPRMETWPDIETRLG
                     EEFERAYHLQTTLDEAIRLSKERTADLFPKK"
     gene            complement(2780..3586)
                     /locus_tag="VF720_01715"
     CDS             complement(2780..3586)
                     /locus_tag="VF720_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7878094.1"
                     /translation="MRPDRIGALALRVGTALLFVLPLWWVLVLSLAPVGGNPSLRLPW
                     WPAAPAFENYPKLFHLVPLARQAANSLFVAAVAVPLTLLTASLAGYSVARMGERWRRR
                     FVIGLVLAMTIPVTALWLTRFVIFRQLGLIDSPLALIAPAIAGTNPLYILLFYRAFRR
                     MPRETFDAARIDGAGHLSTWWRLALPEARPALVTAGVLAFVFYWSDLISPILYLKSEG
                     RYTLPVGLSSLAAMDVTDGPLLMAGIVVITAPVILAFLFAQRFFWPHGGR"
     gene            complement(3576..4448)
                     /locus_tag="VF720_01720"
     CDS             complement(3576..4448)
                     /locus_tag="VF720_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7878095.1"
                     /translation="MRERAVLAAFLMPAAAGMLLLTLLPALVALAFSFSAWDGLSPPE
                     WRGVATFREVLADPYFRMAARNSLVFVLMAVPLRLLGALGLALLYRRSHPGADACRTA
                     AFLPTIVPDAAYALTWLMLLNPLHGPLNIALRAVGLPAPAWLAHGDTALLSIVIMSCF
                     QVGEGMVVLLAGLATIPADWYRAAQLDGAGRWASFRWITLPMILPWLVLLTLRDVIMS
                     AQNTFVPAYLMTGGGPYYATLFVPLHMYQQAFDGFRFGHAAAEMVLLLGAVALLLAIV
                     ARVARRPAIDDDAA"
     gene            4498..>5745
                     /locus_tag="VF720_01725"
     CDS             4498..>5745
                     /locus_tag="VF720_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7878096.1"
                     /translation="MSGYDNILNLLILYDTARGFLPLTESADVIHHALLSFAGAAGTR
                     RVDWFAHQGGGRFEMVPDRGARPVRPPSFQVPAGALARLANDPGPLARTELDDDAEAL
                     ADTAGHLGHALIVPVVEDGRLYGLVGIGDKLLGEFHTDTERSLVHEIAHLMASALLRQ
                     AGTPPGAARSDSNETLGELRKAHPPLVHIAGESQATRALIDEILTLADFDLPVLVMGE
                     TGTGKELVARALHELGPRADGPFEAINCAAIPRELVSSHLFGHEKGAFTGAVGDSRGA
                     FERAGEGTLFLDEIGDMPLDVQASLLRVLQERRFTRVGGEKPLPARARILSATNRNLE
                     EAVAAGTFRADLFYRIQMYSLRLLPLRDRREDIPGIVDHILDEVRRVRGNAPRATPAF
                     LAAVSGRPLAGNVRELEGLVLSAI"
BASE COUNT          954 a         1956 c         2009 g          826 t
ORIGIN      
        1 aagcgccccg cgccggccgc gccgcgtgga gaagccatgc gccgtcatct cgagcacctc
       61 gtcgaacgtg gcgcgatcga gtcgtcgata gggccacgcg cggcccaggg ccaggtagag
      121 atcgtccacc gaccagtctt cggcggccac ctcggccacg atctgctggg ccagcacatc
      181 cagcggcgcc ggctggatgc ggagatgctc cagctccccc gccttcaccg cgcgcaccag
      241 cgccgtgcat tcgaccaggt cttcccgcga gaggggaaag agccgcccct tcggtgtgcc
      301 gtggatggtg tgccccgaac gccccaccct ctgcaagaac gtgctgatcc gccccggcga
      361 gccgagctgc accaccaggt cgaccgagcc gatgtcgatg ccgagctcca gcgacgccgt
      421 ggccacgagg agcgagagcg agccttcctt gaggcgcgtc tcggcatcgt ggcgcttctc
      481 tttcgagagg ctgccgtggt gcgcggtcac tctttcttcg ccgaggcgtc tggacaggtg
      541 gtgcgccacc cgctcgcacc agcgccgggt gttcacgaag accagcgtgg tgcgatgcgc
      601 aaaggccagc accgccaggc gatcgtagat ctcctcccac acgtcgttcg acatcaccgc
      661 ctcgagcggc gaacccggca cctcgatggc caggtcgagc gcccggcgat gcccctcgtc
      721 cacgatggcg cagtcgggcg tgccgtcgcc ggcaacggcg ccgccaccca ccaggtagcg
      781 cgccacttcg tcgatcggtt tctgcgtggc cgacaggccg atgcgctgca gccgcccgtt
      841 ggtgacgtgt tcgaggcgct ccaggctcag cgccaggtgc gcgccgcgcc gcgattcgat
      901 gaccgcgtgg atttcgtcca cgatgaccgt tcgcacggtg gccagcatcc gccggctcct
      961 ctcggccgtg agcagcaggt agagcgattc cggcgtggtg accaggatat ggggcggcgt
     1021 cttgagcatg gccgtgcgtt cccggctcgg cgtatcgccg gtgcgcaccg ccgcggtgac
     1081 ccgcgccgcc ggaaggccca tcgcctcggc cagggcccgg atctcgcggc gcggctcggc
     1141 caggttccgg tggatgtcgg ccgacagcgc cttgagcggc gacacgtaaa ggatgcgcgt
     1201 ctcgtccggc aggggccccg tctccccctc ccggaacagc tcatcgagcg cggagaggaa
     1261 ggccgccagc gtcttgcccg atcccgtcgg ggcggcgatc agcgtgtgcc ttccccggcg
     1321 gatttcggcc cagccgcggg cctgggcggc ggtaggggct ccgaagcggt cggtgaacca
     1381 gcgggagacg gcgggatgga atccggacag ggacatggtc gattgagtgt aacgccgccg
     1441 gcggcggcgg gtaccccgag gatcgggcca catccgccgc ggccggcgcc cccctacttc
     1501 ttcgggaaga gatccgccgt gcgctccttc gacagccgga tggcctcgtc gagcgtggtc
     1561 tgcaggtggt aggcgcgctc gaactcctcg cccaggcggg tttcgatatc gggccaggtt
     1621 tccatgcggg gcacggcccg gatatgcggg atcacgtcca ggaacacctg gctgctggcc
     1681 ggcttgtggt cggtctcgag gaacacctgg gactcggcca cccggcgcat cgagggaacg
     1741 gtgcggccgg tgccgcacat cagcttctgc ccttcctccg agttggcgta ctcgatgaag
     1801 gtccaggccg cgtccttgtg cttcgactgc ttcgacatga agtagccgtc cgagtggagg
     1861 atgccggcgg gttgcttgcc ggtggggagc ggcgccacgt cccaatcgaa gccctgcacg
     1921 ttgcggtaca ccggcacgcc gcgccggctg tcgaagtaca tggccatggc gccgcgcatg
     1981 aagcggtgca cgctgggctc ggccttctcg gccacgaagt cgggcacgat cttgtgcacc
     2041 gactggaatc cgatgaacca ctccagcgcg ccccgcgtct cgggcgtatc gagtgtgagg
     2101 cgcgtgggct tctcgggatc gtcgaagaag tcgccgccgt tctgccagat gaacgggaaa
     2161 acccggaaga ccaccgcctc ggttccgagg ccgtactggt cgatcgtccc atctccgtcc
     2221 gtgtccttcg tcagggcttt ggccgtggcg atgaactcgt cccaggtcca gtccggcttc
     2281 gggtagggga tcccggccgc cgcaaagagg gagcggttgt agtagaccac caggctcgaa
     2341 atgttctgcg ggatggccat ctgcttgccg tcccagcgga agggctccat cgcttcgcga
     2401 tagaagtcgt cctcgtggat cagggcgctc ttcttcaacc gcgagtcgag cggctcgatc
     2461 cgccccttgg cggcaaacgg cgccatccga cgatagttga gcaggaagac gtcggccggc
     2521 gtgcccgcca cgaaatcggt ggtgagccgc tggatgtaga gattctggtt gggcacatgg
     2581 atcaggtcga cgaggatgtc ggggtgcccg gcatgaaacg acgacaccag ctggtcgtag
     2641 gcctgcttct cgaccgggtc gccccagatc atgaaggaga tggtcgtggg ttcgagggcg
     2701 gcgacggggt tggccagggc aagggcggcc aggatcatcg gcaggaagac gtttgttttt
     2761 cgcatcggga gattatcggc taccggccgc cgtggggcca gaaaaaccgt tgggcaaaca
     2821 ggaaggccag gatgaccggt gccgttatca ccacgatgcc ggccatgagg agcggaccgt
     2881 cggtcacatc catggccgcc agggacgaga gcccgaccgg cagcgtgtat cgcccctcgg
     2941 acttgaggta gaggatcggc gagatgagat cgctccagta gaagacgaag gccagtactc
     3001 ctgccgtcac gagggccggc cgggcctccg gcagcgccag ccgccaccag gtggacaggt
     3061 gtcccgcccc gtcgatgcgg gccgcgtcga acgtttcccg cggcatccga cggaaggcac
     3121 ggtagaagag aagaatgtac aggggattgg ttcccgcgat ggcgggcgcg atcagcgcca
     3181 gcggcgagtc gatcaggccg agctggcgga agatcacgaa ccgggtgagc cagagcgccg
     3241 tcaccgggat ggtcatggcc aggacgaggc cgatcacgaa ccggcgccgc caccgctccc
     3301 ccatcctggc caccgagtag ccggccagcg aggcggtgag gagcgtgagc ggcacggcca
     3361 cggccgccac gaagagggag ttggccgcct ggcgggcgag cggtacgagg tggaagagtt
     3421 tcgggtagtt ctcgaaggcc ggcgcggccg gccaccaggg aagccgcagc gaagggttcc
     3481 cccccaccgg cgccagcgat aggaccagca cccaccagag ggggagcacg aacaggagcg
     3541 ccgtccccac gcgcagcgcc agggcgccga tcctgtcagg ccgcatcgtc gtcgatggcc
     3601 ggccggcggg ctacccgggc cacgatggcc aacaggagcg cgacggcgcc gaggaggagc
     3661 accatctccg cggcggcatg accgaagcgg aaaccgtcga atgcctgctg gtacatgtgg
     3721 agcggcacga agagcgtggc gtagtacggc ccgccccccg tcatgagata cgccggcacg
     3781 aaggtgttct gcgcgctcat gatcacgtcg cgcagcgtga gcagcacgag ccacggcagg
     3841 atcatcggca gcgtgatcca ccggaacgac gcccagcggc ccgcgccgtc gagctgcgcg
     3901 gcgcgatacc agtcggccgg aatcgtggcc aggcccgcca gcagcaccac cattccctct
     3961 cccacctgga agcaggacat gatcacgatc gagaggagtg ccgtatcgcc atgggccagc
     4021 caggccggcg cggggagtcc cacggcgcgc agggcgatgt tgagtggtcc gtgcagcggg
     4081 ttgagcaaca tgagccaggt gagggcatac gccgcgtcgg ggacgatggt gggaagaaat
     4141 gccgcggtcc ggcaggcatc ggcacccgga tgcgagcgac gatagaggag ggccaggccc
     4201 agcgcgccca ggagccggag cggcacggcc atgagaacga agaccagcga gttccgggcc
     4261 gccatcctga agtagggatc ggccagcacc tcgcggaacg tggccacccc gcgccactcg
     4321 ggaggggaaa gaccgtccca ggcactgaag gaaaatgcga gcgcgacgag ggcggggagc
     4381 agggtgagga gcagcatccc ggccgcggcc ggcatgagga aggcggccag gaccgcgcgc
     4441 tcgcgcatgc gggaggagac accgccacac cgcatcgcgt caaggtatga tcgggacatg
     4501 agcggctacg acaacatcct caacctgctc atcctctacg acacggcccg gggcttcctg
     4561 ccgctcacgg agtcggccga tgtcatccat catgccctcc tgtcgttcgc cggcgcggcc
     4621 ggtacgcgac gagtcgactg gttcgcccac cagggcggcg gccgcttcga gatggtgccc
     4681 gaccgcggcg cccggccggt ccgcccccct tcgttccagg tgcccgccgg cgcgctggcc
     4741 cggctggcaa acgacccggg ccccctcgcc cgcactgaac tcgacgacga cgccgaggcg
     4801 ctggccgaca ccgccggcca cctcggtcat gcgctcatcg ttcccgtcgt ggaagacggg
     4861 cgcctctacg gcctggtcgg catcggcgac aagctcctcg gcgagttcca caccgacacg
     4921 gaacgctcgc tcgtccacga gatcgcccac ctgatggcct ccgccctcct tcgccaggcg
     4981 ggtacgcctc ccggcgccgc gcggagcgac tcgaacgaaa cgctgggtga gctcaggaag
     5041 gcgcatccgc ccctcgtcca catcgccggg gagagccagg ccacccgggc gttgatcgac
     5101 gagatcctca cgctggccga cttcgacctc cccgtgctcg tcatgggaga aaccggtacc
     5161 ggcaaggagc tggtggcccg ggctctgcac gaattgggcc cccgggccga cgggccgttc
     5221 gaggccatca actgcgcggc catcccgcgg gagctcgtgt cgagccatct cttcggccac
     5281 gagaaaggcg cgttcaccgg agcggtgggc gattcgcgcg gggccttcga gcgggccggc
     5341 gaagggaccc tcttcctcga cgagatcggc gacatgccgc tcgacgtgca ggccagcctc
     5401 ctccgcgtat tacaggaaag gcgcttcacg cgggtcggcg gtgaaaaacc gctgccggcc
     5461 cgggcccgca tcctctcggc caccaaccgg aacctcgagg aagcggtggc ggccggcacg
     5521 ttccgggccg acctcttcta tcgcatccag atgtactcgc tccggctcct ccccctgcgc
     5581 gaccggcgcg aggacatccc gggaatcgtc gaccacatcc tcgacgaggt ccggcgggtc
     5641 cgcggtaacg cgccccgcgc cacgccggcc ttcctggccg cagtgtcggg gcgtccactg
     5701 gccggcaacg tgcgcgagct ggaagggctg gtcctctcgg ccatc
//
LOCUS       DASVOD010000087         4184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_6173, whole genome shotgun sequence.
ACCESSION   DASVOD010000087 DASVOD010000000
VERSION     DASVOD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4184
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6173"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3426)
                     /gene="dnaE"
                     /locus_tag="VF720_01730"
     CDS             complement(<1..3426)
                     /gene="dnaE"
                     /locus_tag="VF720_01730"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX7878097.1"
                     /translation="MTRSGFVHLHNHSDYSLLDGAQTIDTMVERAKSMGMPALALTDH
                     GNMFGAIKFYDACQKAGIQPIVGIEAYVTRGSRTDRTAKHGPQNPLDHLVLLARDEEG
                     YKNLVRLSSASYVEGFYYKPRIDIDLLARHSGGLMGLSACLNAEIPKLLLGGRGEEAF
                     QAAGRFQEMFGKENFYVELQDHDIPEEQQVMPMLVELAKKAGIPLVATNDCHYSRKED
                     ADAQDILVCIQTNKRVNDTDRLKFTTSELYFKSPEEMRTLFRHYPEACDATLDIAEKC
                     KLKLQLGQPQLPHFPCPDGFAGLDDYLAHVSREGLNRRYGTPGDALRERLEFELDVIK
                     KMGYAGYFLVVQDFIDHARSRGIPVGPGRGSAAGSLVAYCTGITDIDPIRFNLLFERF
                     LNPERISMPDIDVDFEDERRDEIIQYVRKKYGEENVCQIITFGTMAARGAIRDVGRAL
                     GFEYGEVDRIAKLVPEELGITLEKAIGLAPDLEKLETEGGEGARLLKTARVLEGLSRH
                     ASTHAAGVIISPRRLDEIVPLFKPANKDEISTQYDMNSVEKIGLVKMDFLGLRTLKVI
                     DRAVRLIQETRGDTIDIGKLEFDDPLAMDLLRAGKAIGVFQVESEGMRNLLRRLQPEN
                     FEDLIAVNALYRPGPMSSGMVDDFIDCKQGRKEVRYEHPLLEPILKPTYGMMVYQEQV
                     MQIASSLAGYSLGQADLLRRAMGKKKAEEMAKQKASFVDGAVANGVAKATAEKIFELM
                     AYFAGYGFNKSHSAAYALISYQTAWLKAHYPVEFMAASLSSEMDKTNRIVILIEECRR
                     MGIPVRPPLVNSSRLDFTVEEGAIRFGLGAVKNVGEGAIDAILAARDGTRAAPGAGTG
                     PFRSLYDFSRRVDLKAVNKRVVESLVAAGAFDDLDGHRAQKMAAVPQAMSAGQSEQDE
                     RSGNQIGLFGGGPAGADAVVSEPKLAEVTPWDQPTLLKEEKGVLGFYLSDHPLAPLRE
                     EIAAVATCDSVTLQERKAGVEIDFVGVVSAVSRRPTRSGSLMAFITLEDFAGSVECIC
                     FSDLYEASKGHLEVDRIVLVRGRADRRGDEDLVKIVADELVPFDESRRQAIHTLHISL
                     PLDSVSERDLDALREILARYPGKGDVVLHFDGGAGRLKVKVGKLKVAVSGDMLG"
     gene            complement(3491..>4184)
                     /locus_tag="VF720_01735"
     CDS             complement(3491..>4184)
                     /locus_tag="VF720_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878098.1"
                     /translation="VSRPVVRHLGGADTLDVIGVPRGGDVQAEFGDLTIQFPDSAAFH
                     PFWLGVSIDSVAGVPGFEPVSRAYRLSAPGVALDKAARFAIRGGPAARNVGLFRKGSN
                     WTWVGNSDAATGTGGDTRYLGTFVLARDTSLPSITILEPSLPGGKKSGPAASRAVLRV
                     RIVDTGSGLAASSIAATLDDVPQLLEWDPEAQVLSGTSRRTLSPGSHRLVVTASDKAG
                     NTAQAERTFTSR"
BASE COUNT          644 a         1386 c         1410 g          744 t
ORIGIN      
        1 accgagcatg tcgccactga ccgccacctt gagcttgccg accttgacct tgagccggcc
       61 ggccccgccg tcgaagtgga gcaccacgtc ccccttgccc ggatagcgcg ccaggatttc
      121 acgcagcgca tcgagatcgc gctccgatac ggagtcgagc ggcagcgaaa tgtggagcgt
      181 gtggatggcc tggcgtcgtg actcgtcgaa cggtaccagc tcgtcggcca cgatcttgac
      241 caggtcttcg tccccgcgcc ggtcggcgcg gccgcgcacc agcacgatgc ggtcgacctc
      301 gaggtgtccc ttcgaggcct cgtagaggtc gctgaagcag atgcactcca ccgaaccggc
      361 gaagtcttcg agcgtgatga aggccatgag gctcccgctc cgggtggggc gccgggacac
      421 ggccgacacg acgccgacga aatcgatctc gacgcccgcc ttcctttcct ggagcgtcac
      481 gctgtcgcag gtggccaccg cggcgatctc ctcgcggagc ggggccagcg ggtgatcgct
      541 caaatagaag ccgagcaccc ccttttcctc cttgaggagc gtcggctggt cccacggcgt
      601 cacctcggcg agtttcggct cgctcaccac ggcgtccgcg cccgcggggc cgccgccgaa
      661 gaggccgatc tggttgccgc tccgctcgtc ctgctccgac tggcccgccg acatggcctg
      721 cggcaccgcg gccatcttct gcgcgcggtg cccgtccagg tcgtcgaacg cgccggccgc
      781 cacgaggctc tccacgacgc gcttgttcac cgccttgaga tcgacgcgcc ggctgaagtc
      841 gtagagcgag cggaacggac cggtgccggc tcccggcgca gcgcgcgtgc cgtcgcgggc
      901 ggccaggatg gcgtcgatgg ccccctcccc cacgttcttg acggcgccca ggccgaatcg
      961 gatggccccc tcctccacgg tgaagtcgag gcggctcgaa ttgacgagcg gcggccgcac
     1021 cggaatcccc atgcgacggc actcctcgat caggatgacg atgcggttcg tcttgtccat
     1081 ttcggagctg agcgaggccg ccatgaattc caccgggtag tgggccttga gccaggccgt
     1141 ctggtacgag atgagcgcgt aggcggccga gtggctcttg ttgaagccgt agccggcgaa
     1201 gtaggccatg agctcgaaga tcttctcggc ggtggccttt gccacgccgt tggccacggc
     1261 gccgtccacg aacgaggcct tctgcttggc catctcctcg gccttcttct tccccatggc
     1321 gcggcggagg aggtcggcct gtccgagcga gtaaccggcc aggctcgagg cgatctgcat
     1381 cacctgctcc tggtacacca tcatgccgta ggtcggcttc aagatgggct cgagcagggg
     1441 gtgctcgtag cgcacttcct tccgcccctg cttgcagtcg atgaagtcgt ccaccatccc
     1501 cgagctcatg gggccggggc gatacagcgc gttgacggcg atcaggtcct cgaagttctc
     1561 cggctggagg cgcctgagga ggtttcgcat cccctccgat tccacctgga agacgccgat
     1621 cgccttgccg gcgcgcagaa ggtccatcgc gagcgggtcg tcgaactcca gtttgccgat
     1681 gtcgatcgtg tcgccgcgcg tctcctggat gaggcgcacg gcccggtcga tcaccttcag
     1741 cgttcggagc cccaggaagt ccatcttcac gaggccgatc ttctctacgg agttcatgtc
     1801 gtactgcgtg gagatttcat ccttgttggc cggcttgaag agcggcacga tctcgtcgag
     1861 gcgccgcggg gaaatgatga cgccggccgc gtgagtggag gcgtgccgcg agagcccttc
     1921 gagcacgcgg gccgtcttga gcagccgcgc tccttcgccg ccttccgttt cgagcttttc
     1981 gagatcgggg gccaggccga tggccttctc cagcgtgatc cccagttcct cgggcaccag
     2041 cttggcgatg cggtccacct cgccgtactc gaagccgagc gcgcgtccca cgtcgcggat
     2101 ggcgccgcgc gccgccatgg tgccgaacgt gatgatctgg cagacgttct cctcgccgta
     2161 cttcttccgg acgtactgga tgatctcgtc acgccgctcg tcctcgaagt cgacgtcgat
     2221 atcgggcatc gagatgcgct cgggattcag gaagcgctcg aagaggaggt tgaagcggat
     2281 ggggtcgatg tcggtgatgc cggtgcagta cgccacgagg ctccccgccg ccgaaccgcg
     2341 ccccggcccc accgggatcc cgcggctccg ggcgtggtcg atgaagtcct gcaccacgag
     2401 gaagtaaccg gcgtacccca tcttcttgat gacgtcgagc tcgaactcga ggcgttcgcg
     2461 cagcgcatcg cccggcgtgc cgtagcggcg attgagtccc tcgcgggaga cgtgcgccag
     2521 gtagtcatcg aggccggcaa atccatcggg gcagggaaag tgcggtaact ggggctggcc
     2581 gagctgcagc ttgagcttgc acttctcggc gatgtcgagc gtggcatcgc acgcctcggg
     2641 atagtggcgg aagagcgtcc gcatctcctc cgggctcttg aagtagagct cggaggtggt
     2701 gaacttgagc cggtcggtgt cgttcacccg tttgttggtc tggatgcaga cgaggatgtc
     2761 ctgcgcgtcg gcatcctcct tccggctgta gtggcaatcg ttcgtggcca ccagcgggat
     2821 gccggccttc ttcgccagct cgaccagcat cggcatcacc tgctgctctt cggggatgtc
     2881 gtggtcctgt aattcgacgt agaagttctc cttcccgaac atctcctgga agcggccggc
     2941 cgcctggaag gcctcctcac cacggccgcc cagcaggagc ttggggattt ccgcgttgag
     3001 gcaggccgag agccccatca atccgcccga atgccgcgcc agcaggtcga tgtcgatgcg
     3061 cggcttgtag tagaacccct ccacgtagct ggcggaggag aggcggacga ggttcttgta
     3121 cccctcttcg tcgcgggcca ggagcaccag gtggtcgagt ggattctgcg gcccgtgctt
     3181 ggcggtgcgg tcggtccggc tccctcgcgt gacataagcc tcgatcccga cgatcggctg
     3241 gatgcccgcc ttctggcagg cgtcgtagaa cttgatcgcg ccgaacatgt tgccgtggtc
     3301 ggtgagcgcc agcgccggca tccccatcga ctttgcccgc tccaccatcg tgtcgatggt
     3361 ctgtgcgccg tcgagcagcg aatagtcgga atgattgtga agatggacga agccggatcg
     3421 ggtcatgaat ggaatttatc accggtcaac gcgcggcgaa agtcgagggt cgcgacgggg
     3481 agccggaccg tcatcgcgag gtgaaggtgc gctcggcctg cgcggtgttt ccggccttgt
     3541 cgctggccgt gaccaccagg cggtgtgatc cgggcgatag cgtccgtcgc gaggttcccg
     3601 acaacacctg ggcctccggg tcccactcga gaagttgcgg cacgtcgtcg agcgtggccg
     3661 cgatggagga ggcggcaagt ccgcttcccg tgtcgacgat gcggacgcgc aggacggcgc
     3721 gcgacgcggc cggccccgac ttcttcccgc caggcaatga cggttcgagg atcgtgatcg
     3781 acgggagcga ggtatccctg gccaggacga aggtgccgag gtagcgcgtg tcgccgccgg
     3841 tgccggtggc ggcgtcgctg ttccccaccc aggtccagtt gctccccttc cggaagagcc
     3901 ccacgttgcg ggccgcgggt ccacccctga tggcgaagcg ggcggccttg tcgagcgcca
     3961 caccgggcgc cgagagccgg taggcgcgtg acaccggttc gaaacccggg acacccgcca
     4021 ccgagtcgat cgatacaccc agccagaagg ggtggaacgc cgcgctgtcg ggaaactgga
     4081 tcgtcaggtc gccgaactcg gcctggacgt cgccgccgcg gggcacgccg atcacgtcga
     4141 gggtgtcggc gccgcccagg tgacgcacca ccggccgact cacg
//
LOCUS       DASVOD010000088         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_318706, whole genome shotgun sequence.
ACCESSION   DASVOD010000088 DASVOD010000000
VERSION     DASVOD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318706"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF720_01740"
     CDS             complement(<1..925)
                     /locus_tag="VF720_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878099.1"
                     /translation="MMIGEIILLALGGALVTLTLLGVAGVPLVAPLVCAVMLLAGLGL
                     VIAVRRAGAVGDRSPWSNRWKAAEILLLAVILAIVGVAGARARFYPVSAMDAHAYDGR
                     ARAIVAERTLDISWYDIPGLDGRSNLSYPPGVPLSLALVYWLGGAEGKIMDILWLGCL
                     LLVLFEFVARRKGRLAGLAAAFLVAAAPEFWRHASLGLTNLATTAAIGAATLAATDRG
                     RPWRAALLFGVATLLRIDSVVVSVSVALALAIGRRRANDLLIALPALAVAIGWQFALA
                     HYGIGSALSALRGSPLPDPSLLVRAVSVAFTD"
     gene            complement(922..1365)
                     /locus_tag="VF720_01745"
     CDS             complement(922..1365)
                     /locus_tag="VF720_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878100.1"
                     /translation="MREYGRLLLVIGLLTLYAAAPGTGPWARSVVTSAGQMYRYRNSG
                     ADRRALSLLGESWRLVDEARRVTPSDARILIPDGPGADPVSNRVWCAYYLHPRHLVRA
                     AEITGRITDAVDFVLVYRGANLERLGVPADSITGDESAIVDLRGR"
     gene            complement(1362..2567)
                     /locus_tag="VF720_01750"
     CDS             complement(1362..2567)
                     /locus_tag="VF720_01750"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7878101.1"
                     /translation="MLKFRRNALFFLTGSFFVGFGLGTYWVLFNLYMKERGFGEEVIG
                     RVLMSGATGTFVMALPAAMIVSRFPTRLVMILAAVTAACGYGLMICPVPLPFIMLGAA
                     LASAAFSAHNIAAAPFFMRNSDPGERLDLFGTHSAIEVAAGVIGAAGGGYLVRFLEPH
                     VGGIEVAYRMTLALASCLVLVAVIPYLGIREEKHAAIVTDLVALLKRAQHGLMARLVL
                     PKFITGLGAALIIPFLNLYFRDRFHLHPDRIGTIFAVSQALTVAAYVAGPHLARRVGR
                     IRAIAGTELLSIPFFVVMALTDRLEFAVAAFWMRGALMNMNQPISGAFAMEMVRPEEQ
                     PLTNALVEFAWHGAWMISTQVGGWLIEHHGYRLPMFITVALYVIASSLYLSFFHDAER
                     KLALRTATA"
     gene            2625..>3300
                     /locus_tag="VF720_01755"
     CDS             2625..>3300
                     /locus_tag="VF720_01755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEX7878102.1"
                     /translation="MSASREVRLATWSRFVKLEHTVFSLPMLVAGGWLAAGGFPGWRI
                     LGLIVLAGFGARIVALGLNRIIDRAIDAANPRTVVRELPRGTMSVAEAWGVVLAGLVV
                     YVAAAWWIAPICLWLSPVPLLVFIVYPYMKRFTPLAHFGVGLGLAVAPLGAWMAVRQT
                     FAGSGPALLLGAFTLFWVAGFDIIYATQDVEFDRKAGLFSIPSRLGVHRALAVSGWLH
                     VAAFLAL"
BASE COUNT          558 a         1114 c         1169 g          459 t
ORIGIN      
        1 ggtccgtgaa agcaaccgac acggcccgca cgagaagcga cggatcgggg agaggggagc
       61 cccgcagcgc cgacagcgcc gaaccgatcc cgtagtgcgc gagcgcaaac tgccagccga
      121 tcgccacggc cagggcggga agcgcgataa ggaggtcgtt ggcccgccgc cggccgatcg
      181 caagcgccag ggccaccgac accgacacga ccaccgagtc gatccgcagg agcgtggcca
      241 cgccgaagag gagtgccgcg cgccacggac gtccccggtc ggtggcggcc agcgttgcgg
      301 cgccgatcgc ggcggtggtg gcaaggttgg tgagcccgag cgaggcatgt cgccagaatt
      361 cgggcgcggc cgccaccagg aaggcggcgg cgaggcccgc cagtcgaccc ttccgccggg
      421 ccacgaactc gaacagcacg aggaggaggc agccgagcca gaggatgtcc atgatcttcc
      481 cctcggcgcc tcccagccag tacaccagtg ccagggagag cggaacgccc ggcgggtagc
      541 tcaggttgct ccggccgtcg agccccggga tgtcgtacca ggagatgtcg agcgtccgct
      601 cggccacgat ggcgcgcgcc cgaccgtcgt aggcatgtgc gtccatggcc gacaccgggt
      661 agaagcgcgc tcgtgcgccc gcaacgccca cgatcgccag gatcacggca aggaggagga
      721 tctcggcggc cttccatcgg ttactccagg gggaacggtc gccgaccgcc ccggcgcggc
      781 ggaccgcgat gacgaggccc agcccggcga gcagcatcac ggcgcagacc agtggtgcaa
      841 ccagcggtac gccggccacg ccgagcagcg tgagcgtgac cagggcaccg ccgagcgcca
      901 ggaggatgat ctcgccgatc atcatcgccc gcgcaggtcc acgattgccg actcgtcgcc
      961 ggtgatcgag tcggccggca cgccgaggcg ctccagattc gcgcctcggt acaccaggac
     1021 gaaatcgacc gcgtcggtga tacgtccggt gatctcggcc gcccgcacga ggtgccgcgg
     1081 atgcaggtag taggcacacc acacccggtt cgacacggga tcggcgccgg gcccgtccgg
     1141 aatcaggatc cgtgcatcgg agggcgtgac gcgccgggct tcgtccacga gccgccagga
     1201 ttcgccaagg aggctcaggg cccgtctgtc ggctccgctg ttccggtatc ggtacatctg
     1261 gccggccgac gtcaccacgc tccgtgccca cggcccggtt ccgggcgccg cggcgtagag
     1321 cgtgaggagg ccgatcacga ggaggagccg gccgtactcc ctcacgcggt ggccgtccgg
     1381 agcgccagtt tccgttcggc gtcgtggaag aacgacaggt agagcgacga cgcaatcacg
     1441 tagagcgcca cggtgatgaa catgggaagc cggtagccgt ggtgctcgat gagccacccg
     1501 ccgacctgcg tcgagatcat ccaggcgccg tgccaggcga actccaccag cgcgttcgtc
     1561 agcggctgct cctcgggccg gaccatctcc atggcgaagg cgcccgagat cggctggttc
     1621 atgttcatca aggcgccgcg catccagaag gcggctaccg cgaactcgag tcgatcggtc
     1681 agtgccatga cgacgaagaa cggaatggac aggagctcgg tgccggcgat cgcgcgaatg
     1741 cgacccaccc gtcgggccag gtgaggcccg gcgacatacg ccgccaccgt gagtgcctgc
     1801 gagacggcga agatcgtgcc gatccggtcg gggtggagat ggaatcggtc gcggaaatag
     1861 agattgagga aggggatgat cagcgcggcg ccgaggccgg tgatgaactt gggcagcacg
     1921 agccttgcca tcagcccatg ctgtgcgcgc ttgagcaggg caaccaggtc ggtgacgatg
     1981 gccgcgtgct tctcctcccg gatgccgagg taggggatca ccgccaccag gacaagacag
     2041 gaagccaggg ccagcgtcat gcggtaggcc acctcgatcc cgccgacatg gggctcgagg
     2101 aatcggacca ggtagccgcc ccccgcggcg ccgatcacgc cggccgccac ttcgatggcg
     2161 gagtgcgtgc cgaagagatc gagccgctcg cccgggtcgg agttccgcat gaagaaaggc
     2221 gcggccgcga tgttgtgggc cgagaacgcc gcgctggcca gggcggcacc cagcatgatg
     2281 aaggggagcg gcacggggca gatcatgagg ccgtagccgc acgcggccgt caccgcggcc
     2341 aggatcatca ccagccgggt gggaaagcgc gagacgatca tggccgccgg caatgccatg
     2401 acgaacgtgc cggtggcgcc ggacatcagg acgcggccga tcacctcttc gccgaagccg
     2461 cgctccttca tgtacaggtt gaagagcacc cagtaggtgc cgagcccgaa tccgacgaag
     2521 aacgaaccgg tcaggaagaa gagcgcgttg cgccggaatt tgagcatcgg cgaagtttac
     2581 aaggggcgcc ccgccccgta ccatccgccg cgatgaacgc cgccatgagt gcctcccggg
     2641 aagtgcggct cgccacctgg agccgattcg tcaagctcga gcacaccgtc ttcagcctgc
     2701 ccatgctcgt tgcggggggg tggctggcgg ccggcggctt tccggggtgg cgcatcctcg
     2761 gcctcatcgt cctggccggc tttggcgccc gcatcgtggc gctcggcctg aatcgcatca
     2821 tcgaccgcgc catcgacgcg gccaacccgc gcaccgtggt gcgggaactg ccgcgcggca
     2881 ccatgtccgt ggccgaggcg tggggcgtgg tgctggcggg actcgtggtc tacgtggccg
     2941 cggcctggtg gatcgcgccg atctgcctct ggctctcgcc ggtgccgctc ctggtcttca
     3001 tcgtctatcc ctacatgaaa cgtttcacgc cgctggccca cttcggcgtg gggctcggcc
     3061 tcgcggtggc gccgctcggc gcctggatgg ccgtccgcca gacctttgcc ggctcgggtc
     3121 cggcgctcct gctcggcgcg ttcaccctct tctgggtggc cggcttcgac atcatctacg
     3181 ccacgcagga tgtcgagttc gaccggaagg ctggcctctt ctcgatcccg tcacgtctcg
     3241 gcgtgcatcg cgcgctggcc gtgtcgggat ggctgcacgt ggccgcgttc ctcgcgctgg
//
LOCUS       DASVOD010000089         8238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_475589, whole genome shotgun sequence.
ACCESSION   DASVOD010000089 DASVOD010000000
VERSION     DASVOD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8238
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475589"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VF720_01760"
     CDS             complement(<1..367)
                     /locus_tag="VF720_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878103.1"
                     /translation="MARSADRRPGSKAARWRRGGAALVLVMATLLAATGAAPKKKADP
                     NEWRRVSVYDVTHSVDRSWSAQVFQSGDFKNLLVVPDEGLDAWALDLAALKGYQLQVM
                     ELGLTEGTARVPSLAAKKPT"
     gene            complement(351..1127)
                     /locus_tag="VF720_01765"
     CDS             complement(351..1127)
                     /locus_tag="VF720_01765"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX7878104.1"
                     /translation="MLLAVDIGNTQTVVGLLEVETIVRQWRLTSQAGRTADEIALQLR
                     GLLDDAALDRAAIRRMAIASVVPNLTPEYRRFGAETLGVPTLVIDGHTDTGVKVDVPD
                     PGSVGPDRIVNVVAALARHGAPAIVIDMGTATTFDALDARGTYVGGAIAPGIGISAEA
                     LFSRGARLARIEIRPPARVIGKTTEEHLQAGIFFGAVGQIDEIVRRMATELGGKPRVV
                     ATGGLSGTVAGYSATIEAVDPDLTLHGIRLVEERHHGPIG"
     gene            complement(1132..1860)
                     /gene="tatC"
                     /locus_tag="VF720_01770"
     CDS             complement(1132..1860)
                     /gene="tatC"
                     /locus_tag="VF720_01770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX7878105.1"
                     /translation="MSFLQHLEELRKAILHSVIVLVIAAALAWPISGWLQEFVITHVT
                     GRMNDVVFMAGKPLGPLSARLKVTLAAALLIAAPFVLFRLWLFVAPGLKTTERRYALP
                     IIATSVVLFYGGFLASFFYMGPQVPEILLGFGTESVQNFIAIDDVMSLVLSLSVACGI
                     VGEVPLVLTFLSWIGIISPESLVRQWRIAVVLILIAAAVVTPGDYGITMLLIALPIAG
                     LYVISIFLAFIVTRKRRARLQASR"
     gene            complement(1927..2391)
                     /gene="nrdR"
                     /locus_tag="VF720_01775"
     CDS             complement(1927..2391)
                     /gene="nrdR"
                     /locus_tag="VF720_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX7878106.1"
                     /translation="MRCPACGHQEDRVIDSRATREGRAIRRRRECLECSHRFTTYEYV
                     EVDTLVVVKKDHRREPFDRQKVISGIARASEKRPISTGDIEDLVDRVVGDLQKVGPEI
                     TTRQIGERIMEELQRLDEVAYVRFASVYRHFKDVNQFMEEIQGLMKSSDEGK"
     gene            2537..4051
                     /locus_tag="VF720_01780"
     CDS             2537..4051
                     /locus_tag="VF720_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX7878107.1"
                     /translation="MSESHAQSRKKLGDILEDCHVVNNAQVQAALAEQRRTGKLFGEA
                     LIDLGFVSEDDLGWALSAQLNLPYIDLSIDMVDRSVVRSLEPELMRKYKIVPLVRVGD
                     ALTVAVADPTNREAIRAVEQATRLTLQVSIASPRRISRIIADLLGPERGDSPDSSDLL
                     FREITRPTVADNDGAPPRPNPLGHLVARALKERVREIHMDPTGDIVRVRFRRGARLEE
                     EPAVSPDDYREMVTRLRRTFGEGVLPVDPVRAALTVSFSEASLRLAMTLVPGTGGESM
                     ILELDETRGAEADPSMLFEEEESAHAVRGWLANSGGLLVVTGPPNAAVRTVWQGLVRS
                     SDRAHRRVALVGLDTGAEVEGVMTLRDTYPDSNVPGQGAFDPIEARGLDVLFAGGVRD
                     EAMLDRMLMAASCGRLIVVRVMAPDATAALALVRDFCRARSLLSRALLGIIEADLDAS
                     GRPRAALMEMNDSLRRALDSGAHPRDLALAAANGGYRALSARLDSQRPDGRAAA"
     gene            4052..5149
                     /locus_tag="VF720_01785"
     CDS             4052..5149
                     /locus_tag="VF720_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018608290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX7878108.1"
                     /translation="MDITELLVFTKEQGASDLHLSSGMPPLVRLNGDMTPLDMPALDR
                     ETLHTLIWDIMNDVQKKTFEEKHELDFAIELAGVARFRVNCFVQRRGEGAVFRVIPSE
                     IKSMQDLNLPPVLADMAMREKGLILVTGPTGSGKSTTLAAMLDHVNRKRRGHIITVED
                     PIEFVHESKNCLINQREIGPHTHSFANALKSALREDPDAILVGELRDLETMQLAITAA
                     ETGHVVFGTLHTNSAAKTIDRVIDIFPSAQQAQIRTMFSESIVGIVCQTLLKRCDKKG
                     RVAGLEILVGVPALRNLIREDKTAQIMSVIQTGGQYGMQSMDQCLKNLVMQGAVTRDE
                     AARKSSNPALFLGGPEAGANEKPGTPQRKAA"
     gene            5162..6274
                     /locus_tag="VF720_01790"
     CDS             5162..6274
                     /locus_tag="VF720_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7878109.1"
                     /translation="MVDVLQLLISMVPTNSSDLYFTSGAPVQRRVNGVCLPASELLTA
                     ADTRAIADFFMSDSQKEEFRVAREMNLAYSIPNVGRYRVNIFVQRGAVGLVIRLVKIQ
                     IPTMDELSLPPMLRDLVMLKKGLVLVTGPTGSGKSTTLASMIDYRNDNDSGHILTVED
                     PIEFIHPHKKSLVTQREVGMDTDSYHAALKNALRQAPDVVLIGEIRDAVAMEAAITFA
                     ETGHLVLGTLHTMNANQTVDRILSFFAREMHALIYLQLSLTLAGIVCQRLIPTVDGQG
                     RRAALEILMTTPRIRDQIHKGEVAALKVSMEESTQEGCQTFDQALYDLYKEGVITLEE
                     ALRNAESANNLRVRIRMENPGGETAESPRLSLHRLR"
     gene            6321..6893
                     /locus_tag="VF720_01795"
     CDS             6321..6893
                     /locus_tag="VF720_01795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEX7878110.1"
                     /translation="MPVATVFGSSTAGPDDPCSREAFALGAGLAGRGFVVRNGGYDGV
                     MAAVSAGARSAGGHVVGVTTAGLDSRRTPNRHLTEVHTEADLFDRLRRLIRGADLFVA
                     FDECGPGTVNEVVLVWALRVVGEIGPDVPLILVGDRWPRLLEGLQGGFSSKWNMDAGT
                     IVVPTTSAALDVIDRRFPRSRSGMAEASGD"
     gene            complement(7193..>8238)
                     /gene="pgl"
                     /locus_tag="VF720_01800"
     CDS             complement(7193..>8238)
                     /gene="pgl"
                     /locus_tag="VF720_01800"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX7878111.1"
                     /translation="GAARGDGGPLGMIPGVLVLPTPADVFDTAARRFTAAVGEALTAR
                     GVAHVVLSGGSTPAGLYQKLALPENQSRLDWKHVHIWWGDERAVPPDDPASNYRLARD
                     TLLRHLELQPSQVHRMAPDAFALEASALRYEAEIDASVPIGEAVGEAVGEAVGEAVIG
                     PAKPLARFDLILLGVGGDGHTASLFPGSGALGVRDRRVAAVPHAPGWPRLTLTLPVIN
                     AARNVHLLATGVAKARAVSRALIGEPANDCPASLIRPTDGQLLWLIDAEADRLRSVGG
                     KGGGRDLGATGGISDPMLRATRGPGDPRNPEDPGDPGSDSSDRARHRLPRELPGPDDG
                     PFIDLPGDSEPES"
BASE COUNT         1372 a         2756 c         2782 g         1328 t
ORIGIN      
        1 aggtcggctt cttcgcggcg agcgacggca cgcgggccgt tccctcggtg agcccgagct
       61 ccatcacctg caactggtac cccttcaggg cggcgaggtc gagcgcccag gcatcgagcc
      121 cttcgtcggg caccaccagc aggttcttga agtcgccgct ctggaacacc tgggccgacc
      181 agctcctgtc caccgaatgc gtcacgtcgt agacgctcac ccgacgccac tcgttcgggt
      241 cggccttctt cttcggggcc gcgcccgtcg cggccaggag ggtggccatc acgagcacga
      301 gggctgcgcc gccccggcgc cagcgcgccg ccttgcttcc gggcctgcga tcagccgatc
      361 gggccatgat gtcgttcctc cacaagtcga atgccgtgca gggtgagatc gggatcgacc
      421 gcctcgatgg tggccgaata gccggccacc gtaccggaga ggcctccggt ggccacgacc
      481 cggggctttc ccccaagctc ggtggccatc cggcggacga tttcgtcgat ctgacccacg
      541 gcgccgaaaa agattccagc ctggaggtgt tcctcggttg tcttgccgat gacgcgggcc
      601 ggcggccgta tctcgatgcg cgccagccgg gcgccccggg aaaaaagtgc ctcggccgag
      661 atcccgatcc ccggcgcgat ggctccgcca acgtacgtcc cacgggcgtc gagcgcgtcg
      721 aacgtggtgg cggttcccat gtcgatgacg atggccgggg cgccgtggcg ggccagcgcg
      781 gccaccacgt tgacgatccg gtcgggcccc accgagccgg gatcgggcac atcgaccttg
      841 accccggtat cggtgtgacc gtcgatcacg agggtcggca cgcccagcgt ttccgcgccg
      901 aagcggcggt actcgggagt gaggttcggc accaccgagg cgatggccat gcggcggatg
      961 gcggcgcggt cgagcgcggc gtcgtcgagc aggccgcgga gctggagcgc gatctcatcg
     1021 gcggtgcggc cagcctggct ggtcaggcgc cactggcgga cgatcgtctc cacctcgagg
     1081 agcccgacca ccgtctgtgt gttgccgatg tccaccgcca ggagcatgga actacctcga
     1141 cgcttgaagc cgcgcgcgac gcttccgcgt cacgatgaag gccaggaaga tgctgatcac
     1201 gtacagcccg gcgatcggca gcgcgatcag gagcatcgtg atgccgtaat ccccgggcgt
     1261 gaccacggcc gccgcgatca ggatgagcac caccgcgatg cgccactgcc ggaccagcga
     1321 ttcgggcgag atgataccga tccacgacag gaacgtcagg accaggggga cctcccccac
     1381 gatgccacac gccacgctga gcgagaggac cagcgacatc acgtcgtcga tggcgatgaa
     1441 gttctggacc gattcggtgc cgaagcccag caggatctcc ggcacctgcg gccccatgta
     1501 gaagaacgac gccaggaagc caccgtagaa gagcaccacc gatgtggcga tgatcggcag
     1561 ggcgtaccgg cgctcggtgg tcttgaggcc gggggccacg aagagccaga ggcggaagag
     1621 cacgaacggg gcggcgatga ggagggccgc ggccagcgtc accttgagac gggccgacag
     1681 gggccccagg ggcttccccg ccatgaagac cacgtcgttc atgcggccgg tcacgtgggt
     1741 gatcacgaac tcctgcagcc acccggaaat gggccaggcg agggcggccg cgatgaccag
     1801 cacgatgacc gaatggagga tggccttgcg gagttcctcg aggtgctgga ggaacgacat
     1861 ctcgggcccc tcggcctccg ggcgccgcgg gatcgagtcc gggacgagtt cgggctgggc
     1921 gatggcctac ttcccctcgt cgctggattt catcagtccc tggatttcct ccatgaactg
     1981 attcacgtcc ttgaagtggc gatagacgga ggcgaaacgg acataggcca cttcgtccag
     2041 ccgctgcagc tcctccatga tccgctcacc gatctggcgg gtggtgatct cggggcccac
     2101 cttctggagg tcccccacca cccggtcgac caggtcctcg atgtccccgg tcgaaatggg
     2161 gcgcttttcg gaggcccggg cgatcccgct gatgaccttc tgccggtcga acggctcccg
     2221 gcgatggtcc ttcttgacca cgacgagggt atcgacctcg acatattcgt aggtcgtgaa
     2281 ccggtgggag cactccaggc actcccggcg tcggcggatg gctcggcctt cgcgggtggc
     2341 gcgggagtcg atgacgcggt cttcctggtg accgcaggcg gggcatctca taaggggttc
     2401 gggcccggaa gtttaccagc ctccccgggg gggcgcaaat cgccgattca cccaactgga
     2461 aactaacggg ctgtcgctcg cggccgataa ctccccccgg ggtcgtgccc catgttcccc
     2521 tccaccggga gattccatga gcgagagcca cgcccaatcg cgcaagaagc tgggcgacat
     2581 cctcgaagac tgtcacgtgg tgaacaacgc gcaggtgcag gcggcccttg ccgagcagcg
     2641 ccggaccggc aagcttttcg gcgaggcgct gatcgacctg ggtttcgtct ccgaggacga
     2701 cctcggatgg gcgctgtcgg cccagctgaa cctcccctac atcgatctct cgatcgacat
     2761 ggtcgaccgg agcgtggttc gaagcctcga gccggagctg atgcggaagt acaagatcgt
     2821 gccactcgtc cgcgtcgggg atgccctgac cgtggccgtg gccgacccga ccaaccggga
     2881 ggccattcgg gcggtggaac aggccacgcg gctcacgctc caggtgtcga tcgcgagccc
     2941 ccggcgcatc tcccgcatca tcgccgacct gctcggcccc gagcggggcg actcgccgga
     3001 ctcgagcgac ctcctcttcc gcgagataac ccgcccgacc gtggccgaca atgacggcgc
     3061 cccgccccgc cccaaccccc tcggccacct ggtggcccgg gcgctcaagg agcgggtgcg
     3121 cgaaatccac atggacccga cgggcgacat cgtccgcgtc cgcttccgcc ggggagcccg
     3181 gctcgaggaa gaaccggccg tttcccccga tgactaccgg gagatggtga cccgcctgcg
     3241 ccgcacgttc ggcgagggag tgctgccggt cgacccggtg cgggccgccc tcaccgtgag
     3301 cttttccgaa gcctccctcc gccttgcgat gaccctcgtg ccgggaaccg gcggcgagtc
     3361 gatgatcctc gaactcgacg agacacgcgg cgccgaagcc gacccctcga tgctgttcga
     3421 ggaagaggaa tccgcccacg ccgtgcgcgg atggctggcc aactcgggcg gcctcctcgt
     3481 ggtgaccggc ccccccaacg cggcggtccg cacggtgtgg caggggctcg tgcgcagctc
     3541 cgaccgggcc catcgccgtg tggcgctcgt gggattggat acgggcgccg aagtcgaggg
     3601 agtgatgacg cttcgcgaca cctaccccga ctccaacgtc cccggccagg gcgccttcga
     3661 cccgatcgag gcgcgcggcc tggatgtgct ctttgccggg ggcgtccgcg atgaagccat
     3721 gctcgacagg atgctgatgg cggcctcgtg cgggcgcctc atcgtggtgc gtgtcatggc
     3781 gcccgatgcc accgcggcgt tggcactggt gcgcgacttc tgccgggcca ggagcctcct
     3841 ctcccgggcg ctcctcggaa tcatcgaggc cgacctcgac gcctccggcc gcccccgggc
     3901 ggccctcatg gaaatgaacg actccctccg gcgagccctc gactccggcg cccatccgcg
     3961 cgacctcgcg ctcgcggccg ccaacggcgg ctaccgggcg ctctcggcgc gcctcgactc
     4021 ccagcggccc gacggccggg cggcggcgta aatggacatc accgaactcc tcgtcttcac
     4081 caaggaacag ggcgcctccg acctccacct gtcgtcgggg atgccgcccc tcgtgcgcct
     4141 gaacggcgac atgacgccgc tcgacatgcc ggcgctcgac cgcgaaacgc tccacacgct
     4201 catctgggac atcatgaacg acgtccagaa gaagacgttc gaggagaagc acgagctcga
     4261 cttcgccatc gaactggccg gcgtggcccg cttccgcgtg aactgcttcg tgcagcggcg
     4321 cggggagggg gcggtattcc gcgtcatccc gtcggaaatc aagagtatgc aggacctgaa
     4381 cctccccccc gtcctggccg acatggccat gcgggagaag gggctgatcc tcgtgaccgg
     4441 gccgaccggt tcgggcaagt ccaccacgct ggccgccatg ctcgaccacg tgaaccgcaa
     4501 gcggcggggc cacatcatca ccgtggaaga cccgatcgag ttcgtgcacg agtcgaagaa
     4561 ctgcctcatc aaccagcgcg agatcggccc ccacacccac tcgttcgcca acgccttgaa
     4621 gagcgcgctc cgtgaagacc cggacgcgat cctggtcggc gagttgcgcg acctggaaac
     4681 gatgcagctg gccatcaccg ccgccgagac ggggcacgtg gtgttcggca cgctccacac
     4741 gaactcggcg gccaagacga tcgaccgcgt gatcgatatc tttccttcgg cccagcaggc
     4801 ccagatccgg acgatgttct cggagtcgat cgtcggcatc gtctgccaga cgctgctcaa
     4861 gcgctgcgac aagaaggggc gggtggccgg gctcgagatc ctggtgggcg tgccggcgct
     4921 ccggaacctg atccgcgagg acaagacggc gcagatcatg tccgtcatcc agaccggcgg
     4981 ccagtacggc atgcagagca tggaccagtg cctcaagaac ctggtcatgc agggggcggt
     5041 gacgcgcgac gaagccgcac gcaagtcttc gaacccggcg ctctttctcg gtggccccga
     5101 ggcgggcgcc aacgagaaac ccggcactcc ccagcgtaag gctgcctaga aagggtcgcg
     5161 cgtggtcgac gtcctccagc tcctgatctc gatggtgccg acgaattcgt cggatctcta
     5221 tttcacctcc ggcgcgccgg ttcagcgccg ggtgaatggc gtctgcctgc cggcgtcgga
     5281 actgctgacg gccgccgaca cgcgagccat cgccgatttc ttcatgagcg actcccagaa
     5341 ggaagagttc cgggtggcca gggaaatgaa cctggcctac tccatcccca acgtgggtcg
     5401 ctaccgcgtg aacatcttcg ttcagcgggg cgccgtcggg ctcgtcattc gcctcgtcaa
     5461 gatccagatc ccgacgatgg acgagttgag cctgccgccg atgctccgag atctcgtcat
     5521 gctcaagaag gggctggtgc tcgtgaccgg tcccaccggt tccggcaagt cgaccacgct
     5581 ggcgtcgatg atcgactatc ggaacgacaa cgactccggc catatcctga ccgtggaaga
     5641 cccgatcgag ttcatccatc cccacaagaa atcgctcgtg acgcagcgcg aggtgggcat
     5701 ggatacggac tcgtaccacg cggcactcaa gaatgcgctg cgccaggcgc ccgacgtggt
     5761 gctcatcggc gaaatccggg atgccgtggc catggaagcg gcgatcacct tcgccgaaac
     5821 cggccacctc gtgctcggca cgctgcacac gatgaacgcc aaccagaccg tggaccgcat
     5881 cctctcgttc tttgcccgcg agatgcatgc gctgatctac ctgcagttgt cgttgacgct
     5941 cgcgggcatc gtgtgccaac gcctgatccc gaccgtggat ggccagggtc gccgcgccgc
     6001 cctcgaaatc ctgatgacca ccccccgcat ccgcgaccag atccacaagg gcgaagtggc
     6061 ggcgctgaag gtctccatgg aggagtcgac gcaggagggg tgccagacct tcgaccaggc
     6121 cttgtacgac ctgtacaagg agggggtgat cacgctcgag gaggcgctgc ggaacgccga
     6181 gagcgcgaac aacctccgcg tgcggatccg gatggagaac ccggggggcg agacggcgga
     6241 atcgccccgg ctgtcgttgc atcggttgag gtaggcgagg cgggtcgatc cgcttccacg
     6301 gcgcgtgata gcttcctcac atgcctgtcg ccactgtctt cggctccagc accgccggtc
     6361 cggatgatcc ctgctcccgt gaggcgtttg cgcttggcgc cggcctcgcc ggccgcgggt
     6421 tcgtcgtgcg gaacggcggt tacgacgggg tcatggcggc ggtcagtgcc ggcgcccgct
     6481 ctgcgggcgg acatgtcgtc ggcgttacga ctgccggcct cgattctcgc cggaccccca
     6541 atcgccacct gactgaagtg catacagaag ccgacctgtt tgaccggttg cgccggctca
     6601 tccgtggcgc tgatcttttc gtggcatttg acgagtgcgg gcccggcacg gtgaacgagg
     6661 tggtgctcgt gtgggcgctg agggtggtcg gtgaaatcgg gccggacgtg ccgttgatcc
     6721 tggtaggtga tcgttggcct cgattgcttg aggggcttca gggggggttc tcgtcgaagt
     6781 ggaacatgga tgctggcacg atcgttgtgc cgaccacgtc cgcggcgctg gatgtgattg
     6841 accggcggtt tccgaggagc aggtccggga tggcggaagc ctccggggac tgattggaaa
     6901 tcagtttgat gaactcggtt tgaaccggat ccgtaccctg attcacaaat atcgagaagg
     6961 ctcgtggagc aagccaccga gctgtcttgt cgagtgctga atcgacggtg tgacatcctg
     7021 ccttccatcg ttacctgttc cggaacaggt tctccgaggg aggagttttc gggcgagggt
     7081 tccggacatt cgttacctgt tccggaacag gttcctgaga gagggcactt caggcgaggg
     7141 gcgacggacg ttcgttacct gttccggaac aggttctccg agagcagagt tttcacgact
     7201 ccggttcgct gtcgccgggg agatcgatga aggggccgtc atcggggccc ggaagttcgc
     7261 gcgggagtcg gtgccgtgcg cggtcgctgg agtcggatcc gggatctcca ggatcttcgg
     7321 gatttctggg atcaccgggt ccgcgcgtgg ctcgcagcat cggatcgctt atcccgccgg
     7381 tcgcgccgag gtcgcgcccc ccgcctttcc cgcccaccga acgcaaccgg tcggcttcgg
     7441 cgtcgatcag ccacaggagc tggccgtcag tcggtcgaat caggctggca gggcagtcgt
     7501 tggcgggttc gccgatcaac gcacgggaaa ccgcacgggc ttttgccacg ccggtggcaa
     7561 ggaggtggac gttgcgggcc gcgttgatca ccggcaacgt gagggtgagg cgtggccatc
     7621 ccggcgcgtg gggaaccgcg gccacgcggc gatcgcgcac tccgagggcg ccacttccgg
     7681 ggaagagcga ggccgtgtgg ccatccccgc cgacgccgag caggatgagg tcgaatcgcg
     7741 cgagcggctt cgccggcccg atgaccgcct cgcccaccgc ctcgcccacc gcttccccca
     7801 ccgcttcccc gatcggaact gaggcatcga tctcggcttc gtagcgcagg gcggaagcct
     7861 cgagtgcgaa ggcatccggc gccatccggt ggacctggga tggttgcagc tcgagatggc
     7921 ggaggagagt gtcgcgggca agtcgatagt tgctggcggg gtcgtcgggc ggaacggcgc
     7981 gttcgtcccc ccaccagata tgcacatgct tccagtcgag gcgtgactga ttctccggca
     8041 gggcgagctt ctggtacagc cccgccggcg tggagccacc ggaaaggacc acgtgcgcca
     8101 cgcctcgcgc ggtgagggcc tcgccgactg cggcggtgaa gcgccgggcg gccgtatcga
     8161 agacgtcggc cggtgtcggg aggacgagga cacccggaat catgccgagg ggccctccat
     8221 cgccgcgcgc agcgcccg
//
LOCUS       DASVOD010000090         4135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_840881, whole genome shotgun sequence.
ACCESSION   DASVOD010000090 DASVOD010000000
VERSION     DASVOD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840881"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VF720_01805"
     CDS             complement(<1..473)
                     /locus_tag="VF720_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX7878112.1"
                     /translation="MFKSLHSWMLVGGLLGVALGIGAHLTLGGTPGLDAFIRNVSQPV
                     GQVFLRLLFMLVIPLIISALGLGIAGLNDLSSLGRVGLKTLAYTVVVSAIAVLIGLTL
                     VNVLKPGSGLPPAAREKLMAGAAERASGLSTSVTAPKTGVDLLVQIVPDNPVKAAA"
     gene            complement(606..1268)
                     /locus_tag="VF720_01810"
     CDS             complement(606..1268)
                     /locus_tag="VF720_01810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX7878113.1"
                     /translation="MSIQLGQEAPNFEAQTTEGTIKFHDWIGDSWAVLFSHPKDFTPV
                     CTTELGYMARVKPEFEKRNTKIIGLSVDPVDRHAQWAADIKETQGHAPNYPMIGDTDL
                     SISKLYGMLPATAGESCDGRTAADNQTVRNVFVIGPDKKVKLILVYPMTTGRNFDEVL
                     RVIDSLQLTAKHKVATPVNWTHGNDVIIAGSVSDEDAKKTYPDGWKSPKPYIRIVPQP
                     KG"
     gene            complement(1401..2309)
                     /locus_tag="VF720_01815"
     CDS             complement(1401..2309)
                     /locus_tag="VF720_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878114.1"
                     /translation="MRDSRPLLITFIVVLAAIGVFYQQKDAARRRAARIETPAPADSF
                     PDADQDGAPDPLPVRRLAGSPEALPALEAVAAAGGQAAWQARPAFAVDIEEVYFDDRG
                     LIREERTSRLVMSRLEPRFVLITSDGRARLGIGDTGPWFAARGDNGLWAPGYPPGVED
                     DGGLRDVAQRVSWLHRLPFVLGDVEVTLEAPPSGEPPDSLIHLTARRPAAPGDTTGAR
                     IDLGFDPATRHLVACRYLDASPLLLVRFDDFRAETPGGLVRPHRWSLFEQDSVAEESR
                     HVLEMRLGDIDWNIAATRADFQPPAS"
     gene            complement(2306..3283)
                     /locus_tag="VF720_01820"
     CDS             complement(2306..3283)
                     /locus_tag="VF720_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7878115.1"
                     /translation="MTQFAHVAPATLEEAARLAAEEGHRLLAGGTDLLREIQAGSAAP
                     DTLVSLRAVPGLDRIERTGKGGLLIGARVSLAEVATHAAIRRDYTALAEAALGAATPQ
                     LRNKATLAGNLLQRPTCEYYRTDFRCALKGGADCPARIGDNTHHALFGGGLCVSAHPS
                     DAAAALHALDARVHLFGNAGASIRPIADLHRKPTASRPTEIALAPGTIVTAIELPPPA
                     GRSTYRQARERATWAFALVGVAARLHLNGTRIRSAGLALTGVAPIPWRLAAVEAWLKG
                     KSIAKDGWAAEAGRRALVGARPLEHNRYKLALVEGLVVRALNALVGRSR"
     gene            complement(3280..>4135)
                     /locus_tag="VF720_01825"
     CDS             complement(3280..>4135)
                     /locus_tag="VF720_01825"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7878116.1"
                     /translation="VMAGTQDLGTGTKTVLAQVAAEEFGVPLARVRVIIGDTGTSAYA
                     PASWGSMTVPSVAPAVRLAAADARRQVEDLIRETSPRKGKGAARSSKSRNAHTPLSPD
                     TLAALRTTLGDFTIIGRGSRWPNPPGKRVAAFAAQWVEVSVDGVTGEVRVTRALSVHD
                     CGRVLNDRLATSQVEGGFIQGLGFALMEGRRIDPNTHKVLNPSFLEYKVPTVRDVPPI
                     DVIFLDEPDTSANNTGAVGIGEPPVIPTAAAIGNAIADACGIRLTETPFTPKRVLDAL
                     ALLGTEPA"
BASE COUNT          601 a         1378 c         1526 g          630 t
ORIGIN      
        1 gccgccgcct tgaccgggtt gtcgggaacg atctgcacga ggaggtcgac gccggtcttc
       61 ggcgccgtga ccgacgtcga gaggcccgac gcccgctccg ccgcaccggc catgagtttc
      121 tcgcgcgcgg ccggcggaag ccccgatccc ggcttgagca cgttcaccag cgtgagcccg
      181 atcagcaccg cgatggccga cacaaccacc gtgtaggcca gcgtcttgag ccccacccgt
      241 cccaggctcg acaagtcgtt gagtccggcg atcccgagcc cgagcgccga gatgatgagc
      301 gggatgacca gcatgaacag gaggcgcagg aagacctgcc cgactggctg gctgacattc
      361 cggatgaacg cgtcgaggcc cggcgtaccg ccaagtgtca ggtgcgcgcc gatgccgagc
      421 gcgacgccga gcaggccgcc gacgagcatc caggagtgca gggacttgaa cacgggcggc
      481 agtatggcga ggggggcggg aggtgacaat aggtcgggcg tctctcccac ggaattcgcc
      541 cacatcaaat aggaggcgcg ggagccgttg gactcccgcg cctcctgaag atcaaccgag
      601 aacgatcaac ccttcggctg cggcacgatc cggatatagg gcttcgggct cttccagccg
      661 tccgggtagg tcttcttcgc gtcctcgtcc gataccgagc ccgcgatgat gacgtcgttg
      721 ccgtgcgtcc agttcaccgg cgtggccacc ttgtgtttgg ccgtgagctg cagcgaatcg
      781 atgacgcgca gcacttcgtc gaagttgcgg ccggtggtca tcgggtagac gaggatcagc
      841 ttgaccttct tgtccgggcc gatgacgaag acattgcgga cggtctggtt gtcggcagcg
      901 gtgcggccgt cgcagctctc gcccgcagtg gccgggagca tgccgtagag cttggagatc
      961 gacaggtcgg tgtcgccgat catcgggtag ttcggcgcgt gcccctgcgt ttccttgatg
     1021 tcggcggccc actgcgcgtg acggtccacc ggatcgacgg agaggccgat gatcttggtg
     1081 ttgcgcttct cgaactccgg cttgaccctg gccatgtagc cgagctcggt ggtgcagacc
     1141 ggcgtgaagt ccttgggatg cgagaagagg acggcccacg aatcgccgat ccagtcgtga
     1201 aacttgatgg tgccttcggt cgtctgcgcc tcgaaattcg gggcctcctg gcccagctgg
     1261 atgctcatgt tcctaccctt cctgtggttc gcggccggga cggccgcccg gagtgggaca
     1321 gcaggtccct gttcaggtct gaacggggaa ttctcccgtt ttccggggac gcgcgcaatc
     1381 gggagtcggc gggggcgcgg tcagctcgcg ggaggctgaa aatcggcacg ggtggcggcg
     1441 atgttccagt cgatgtcgcc gaggcgcatc tcgagcacat gccgggattc ctcggcgacg
     1501 gaatcctgtt cgaagaggct ccagcggtgg ggccggacca ggcccccggg cgtctcggcg
     1561 cggaagtcgt cgaatcggac caggaggagg ggactcgcgt cgaggtagcg gcaggccacc
     1621 aggtggcggg tggcggggtc aaagcccagg tcgatgcggg ccccggtggt atcgccgggc
     1681 gccgcgggac gccgggccgt gaggtgaatg aggctgtcgg gcggctcgcc ggacggcggc
     1741 gcctcgaggg tcacctccac gtcccccagg acaaacggca gccggtggag ccaggaaact
     1801 cgttgtgcca cgtcgcggag ccctccgtcg tcctcgactc cgggcgggta gcccggcgcc
     1861 cacaggccgt tgtcgcctct cgcggcgaac cagggacccg tgtccccgat gccgagccgc
     1921 gcccggccgt ccgaggtgat gaggacgaac cggggctcca accgggacat caccagccgg
     1981 ctcgtgcgct cctcgcggat caggccacgg tcgtcgaagt agacttcctc gatgtcgacg
     2041 gcgaaggccg ggcgcgcctg ccaggccgcc tgaccgccgg ccgctgccac ggcctcgaga
     2101 gccgggagcg cctccggact tccggccagg cgtcgcaccg ggagcgggtc gggcgcaccg
     2161 tcctggtcgg catcgggaaa cgagtcggcg ggggcggggg tttcgattcg cgcggcccgg
     2221 cgacgggccg catccttctg ctgatagaag acgccgatcg cggcgaggac gacgatgaag
     2281 gtgatgagga gcggccggct gtccctcacc gggagcggcc gacgagcgcg ttgagcgctc
     2341 gaaccacgag cccttcgacc agtgccagct tgtagcggtt gtgctcgaga ggacgtgcgc
     2401 cgacgagggc gcgccggccg gcttcggcgg cccacccgtc cttcgcgatc gatttcccct
     2461 tgagccaggc ctcgaccgcg gcgagcctcc aggggatcgg ggccacgccg gtgagtgcaa
     2521 ggccggcgct ccggatgcgc gtgccgttca ggtgcaggcg ggcggccacg ccgaccagcg
     2581 cgaaggccca ggtggcccgc tcgcgggcct gccggtaggt gctccggccc gccggcggcg
     2641 gcagttcgat ggcggtcacg atcgtgccgg gtgccagcgc gatctcggtc gggcgcgagg
     2701 ccgtgggctt ccggtgcagg tcggcgatcg ggcggatcga cgcgccggcg tttccgaaca
     2761 ggtggacacg ggcgtcgagc gcgtgcaggg ccgccgcggc gtcgctcgga tgggcgctga
     2821 cgcagaggcc gccaccgaag agggcgtggt gcgtgttgtc gccgatccgg gcgggacagt
     2881 cggcccctcc cttgagcgcg caacggaagt cggtacggta gtattcgcag gtcggccgct
     2941 ggaggaggtt tccggcgagg gtggccttgt tccgaagctg tggcgtggcc gcgccgaggg
     3001 cggcctcggc cagtgccgtg taatccctgc ggatggcggc gtgggtggcc acttcggcca
     3061 gcgacacgcg ggccccgatg aggaggccac ccttccccgt tcgctcgatc cggtcaaggc
     3121 cgggaacggc ccgcaggctg acgagcgtgt cgggcgcggc ggagccagcc tggatttccc
     3181 gcaggaggtc ggtgccgccg gcgaggagtc gatgcccctc ctcggccgcg aggcgggccg
     3241 cttcctccag cgtggcgggc gccacatgcg caaattgcgt cacgccggct ccgtcccgag
     3301 cagtgccagc gcatcgagca cccgcttcgg cgtgaagggc gtttcggtca gccggatgcc
     3361 gcaggcgtcg gcgatggcat tgccgatggc cgccgccgtc gggatgaccg gcggctcgcc
     3421 gatgccgacc gcgcccgtgt tgttggcgct cgtatcgggc tcgtcgagga agatcacgtc
     3481 gatcggcggc acgtcgcgca ccgtcggcac cttgtattcg aggaacgacg ggttgagcac
     3541 cttgtgcgtg ttcgggtcga tgcggcgacc ttccatcagc gcgaagccga gcccctggat
     3601 gaagccccct tccacctggc tcgtggccag ccggtcgttc aggacgcggc cgcagtcgtg
     3661 caccgacagc gcgcgggtga cgcgcacttc gccggtcacg ccatcgaccg acacctccac
     3721 ccattgcgcc gcgaacgccg ccacgcgctt gccgggcggg ttgggccagc gcgagccgcg
     3781 gccgatgatg gtgaaatcgc cgagcgtcgt gcggagggcg gcgagcgtgt cgggggagag
     3841 gggcgtgtga gcgttgcgcg acttcgagct acgcgcggcg cccttcccct tgcggggcga
     3901 cgtctcgcgg atcagatcct caacctgccg ccgcgcgtcg gccgccgcca gccgaaccgc
     3961 cggcgccacc gagggaaccg tcatcgagcc ccacgaggcc ggcgcatagg ccgacgtgcc
     4021 ggtatcgcca atgatgacac gcacccgcgc gagcggcacg ccgaactcct cggccgccac
     4081 ctgcgccagc accgtcttcg tgccggtccc caggtcctgc gtgccggcca tcacg
//
LOCUS       DASVOD010000091         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_736431, whole genome shotgun sequence.
ACCESSION   DASVOD010000091 DASVOD010000000
VERSION     DASVOD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736431"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1166
                     /locus_tag="VF720_01830"
     CDS             183..1166
                     /locus_tag="VF720_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7878117.1"
                     /translation="MSLDCAQGIRVVSPASVRRAAPRVVASAVGALLLMAGAGVASAC
                     SICRCGDPTFNALGRTGYSTPGFRSALDWERFDKDEGNPEEDFESQVENRVTGLLSYG
                     LSDRAMFTLRVPYSHRELTETEDGDEESVVTDGFSDPELSAQVRLWSTSGATMANGTS
                     VSLYGGVKMPFGENALMEDGERIDEHAQPGTGSTDFHGALNLLHLFGTQSSMFASAGY
                     RANGTNDFDYHYGDAWLANLAYEHKLGDRLDGVLEFNYRHAQADEIDPDGTEEENTGG
                     SLLYVTPKLAANLGRNFVLRTAAQIPTVKDLNGEQTERVVANVGMTYFLSH"
     gene            1340..1612
                     /locus_tag="VF720_01835"
     CDS             1340..1612
                     /locus_tag="VF720_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878118.1"
                     /translation="MFNDQMTREADAHAEGETARMIEGQTAKLPSDLFLWAGLGALGT
                     AAIMSMAGRRHGGLQVGQFAAPLLIMGLYDKLVKVAGSDRVAAPEV"
     gene            complement(1685..2218)
                     /locus_tag="VF720_01840"
     CDS             complement(1685..2218)
                     /locus_tag="VF720_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878119.1"
                     /translation="MMLKRIAADAIPAALARAERYRLLNQARQAESICRDVLEVDPNH
                     GEALVLLLLSLTDCFADEGAEAAHADALALLPKLSDGYARDYYQGLIVERWALALAGR
                     AAPTHLVADWIHRAMASFEKAEAQRPAGNDEALLRWNACARLLGRLSRSGSAGASHQA
                     APADRPDELDTGDDPPG"
     gene            complement(2247..2984)
                     /locus_tag="VF720_01845"
     CDS             complement(2247..2984)
                     /locus_tag="VF720_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX7878120.1"
                     /translation="MSAVTFRVWRGTKDEGKPVEYTTELGEGMVVLDAMHKIQAEQAP
                     DMAIRWNCKAGKCGSCSAEVNGKPRLMCMTRMDEIDTSQPVTVEPMKAFPLIRDLVTD
                     VSWNFEVKKRITPFKPRKPDDPDGSWRIDQSDTERVQEFRKCIECFLCQDVCHVLRDH
                     HLHDKFIGPRFLVYAAALEMHPLDVADRRDDLKDAHGIGFCNITKCCTKVCPEHITIT
                     DNAIIPLKERVADRHYDFLKRLLGGGK"
     gene            complement(2984..>3269)
                     /locus_tag="VF720_01850"
     CDS             complement(2984..>3269)
                     /locus_tag="VF720_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX7878121.1"
                     /translation="RLVGVDGNRHYNPGWHLALDMKNMLIVSEAITKSALERKESRGA
                     HFREDFPDKSAEAARFNLMIKRNADGSMNLSKVPVPELPAEHQAIIKEQA"
BASE COUNT          504 a         1095 c         1098 g          572 t
ORIGIN      
        1 ggcagcggcg aaacaggttc cgggtgcccg tgtgcgggcg tacaatcttc cctcgagagg
       61 tcgcgtctgg gattggctgc tcgatgccgg cgtcgatctg atcggcgtcg acgaccccgc
      121 cgaagggtcc cggcttctcg ccaggcggac ccgctgagcc accttccccc acggagagct
      181 gcatgagcct ggattgcgct cagggtatcc gcgtcgtttc gcccgcatct gtccgccgcg
      241 ccgcgccccg cgtcgtggcg tccgccgtcg gcgcgctgct cctgatggcc ggtgccggcg
      301 tcgcgtcggc ctgctcgatc tgccgttgcg gcgatccgac gttcaacgcg ctcggccgga
      361 cgggctattc cacccccggc ttccgcagcg cgctggactg ggaacgcttc gacaaggacg
      421 aggggaatcc cgaggaagac ttcgagagcc aggtggagaa ccgggtgacc ggactccttt
      481 cgtatggcct cagcgaccgg gccatgttca ccctgcgcgt tccgtacagc catcgcgagc
      541 tcaccgaaac ggaagatggg gacgaggagt cggtcgtcac cgacggcttc tcggatcccg
      601 agctctcggc gcaggtgcgc ctctggtcga cgagcggcgc cacgatggcc aacggtacgt
      661 cggtgtcgtt gtatggcggc gtcaagatgc ctttcggcga gaacgcgctc atggaagatg
      721 gcgagcggat cgacgagcac gcccagcccg ggaccggctc gaccgacttc cacggggcgc
      781 tcaacctgct ccacctgttc ggcacccagt cgtcgatgtt cgcgtcggcc ggctaccgcg
      841 ccaacgggac caacgacttc gactaccact acggcgatgc ctggctggcc aacctggcct
      901 acgagcacaa gctcggcgac cggctcgacg gcgtcctcga gttcaactac cgccacgccc
      961 aggccgacga gatcgacccg gacggcaccg aggaagaaaa caccggcggc agcctccttt
     1021 acgtgacgcc gaagctggcc gcgaacctcg gccggaattt cgtcctgcgc accgccgccc
     1081 agatcccgac ggtgaaggac ctgaacggcg aacagaccga gcgcgtggtg gccaacgtag
     1141 gaatgaccta cttcctcagc cactgagaaa gggtgtcggt tcgatacccg gccacgggcg
     1201 ccaatccgtg acaatcctcc gcgatttccc cggaatgcaa cttgcaggtg ccggtctgtt
     1261 gccgaccgac cggtcggcag gatcctgtct tcatatcagg aaaatcccga cgtttcacgg
     1321 gatttccggg gagatcacca tgttcaacga tcagatgacc agggaagccg acgcgcacgc
     1381 cgaaggggaa acggcccgga tgatcgaggg tcagacggcc aagctgccgt ccgacctctt
     1441 cctgtgggcg ggcctcggcg cgctcggcac cgccgccatc atgtccatgg ccggacgccg
     1501 gcacggcggg ctgcaggtcg gccagttcgc cgctccgctc ctcatcatgg ggctgtacga
     1561 caagctggtg aaggtggccg gctccgatcg ggtggcggcg ccggaggtgt agtcggggag
     1621 gtcggcccag tcgggtcacg atggcgggct ttggacagtc gggtaacgat cgactggccg
     1681 cctctcaacc cggcgggtcg tccccggtgt cgagttcgtc cggccggtcc gccggggccg
     1741 cctggtgtga ggctccggcg cttcccgagc ggctcaatcg cccgaggaga cgcgcgcagg
     1801 cgttccagcg gaggagcgcc tcgtcgttcc cggccggacg ctgcgcctcg gccttctcga
     1861 acgacgccat cgcgcgatgg atccagtcgg ccaccaggtg cgtgggcgcg gcccggcccg
     1921 ccagggccag ggcccagcgc tcgacgatca gcccctggta gtagtcacgg gcgtatccgt
     1981 cgctcagttt cggcagcagg gccagcgcat ccgcgtgggc ggcctcggct ccctcgtcag
     2041 cgaagcagtc ggtgagcgag aggaggagaa gcaccagcgc ctcgccatgg ttcggatcga
     2101 cttccaggac gtcgcggcag atgctctcgg cctggcgcgc ctggttgagc aggcggtagc
     2161 gctcggcccg ggccagcgcc gcgggtatgg cgtcggccgc aatcctcttc aacatcatgc
     2221 catccctccg ttcaggcggg gcgccatcac ttgcccccgc cgagcagccg cttcaggaag
     2281 tcgtaatgcc tgtccgccac gcgctccttc agcgggatga tggcgttgtc ggtgatggtg
     2341 atgtgctcgg ggcagacctt ggtgcagcac ttggtgatgt tgcagaagcc gatgccatgg
     2401 gcgtccttca ggtcgtcgcg gcgatcggcc acgtcgagcg gatgcatctc gagcgcggcg
     2461 gcgtatacca ggaagcgcgg cccgatgaac ttgtcgtgca ggtgatggtc gcgcagcacg
     2521 tggcagacgt cctggcacag gaagcactcg atgcacttgc ggaattcctg cacgcgctcc
     2581 gtgtcggact ggtcgatccg ccacgagccg tccgggtcgt ccggcttgcg tggcttgaac
     2641 ggcgtgatcc gtttcttgac ctcgaagttc cacgacacgt ccgtgaccag gtcgcggatc
     2701 aaggggaagg ccttcatcgg ctccaccgtc accggctggc tggtgtcgat ctcgtccatc
     2761 cgcgtcatgc acatgaggcg cggcttgccg ttgacctccg cggaacagga gccgcacttt
     2821 cccgccttgc agttccagcg gatggccatg tcgggcgcct gctcggcctg gatcttgtgc
     2881 atggcgtcga ggaccaccat cccctcgccg agctcggtgg tgtactcgac cggcttccct
     2941 tcgtccttcg tcccgcgcca gacgcggaag gtgacggctg acatcacgcc tgctccttga
     3001 tgatggcctg gtgctcggcc ggcagctcgg gcaccggcac tttcgacagg ttcatgctgc
     3061 cgtccgcgtt ccgcttgatc atgaggttga agcgggccgc ctcggcggac ttgtcaggga
     3121 aatcctcgcg gaagtgggcg ccgcggctct ctttccgttc gagcgccgac ttcgtgatcg
     3181 cctcggacac gatgagcatg ttcttcatgt cgagcgccag gtgccagccg gggttgtagt
     3241 ggcgattgcc gtccacgccg acgagtcgc
//
LOCUS       DASVOD010000092         1677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_318950, whole genome shotgun sequence.
ACCESSION   DASVOD010000092 DASVOD010000000
VERSION     DASVOD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318950"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..610)
                     /locus_tag="VF720_01855"
     CDS             complement(314..610)
                     /locus_tag="VF720_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878122.1"
                     /translation="MTPEPPVDSSADSTEPDSTERRRQRLPEVRWSELNSPGCYLLTR
                     VGDLLRVTEEMLRQRSAHPGLRGSTRLARLSDDPHEVLPVLRDIAGTSGYFVNF"
     gene            complement(714..>1677)
                     /locus_tag="VF720_01860"
     CDS             complement(714..>1677)
                     /locus_tag="VF720_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7878123.1"
                     /translation="LRERLRERNAIGHIVSEDAKMRAVLDLVAQVGPSKASVLITGES
                     GTGKELVARALHAGHPGRTGRFLAVNCSAIPENLIESLLFGHRRGSFTDARDDRPGLF
                     QEANGGTIFLDEVGDMPPALQGKLLRVLQEKEVTPVGATQPVPVDVRVVAATHRDLAQ
                     RAKEGSFREDLYYRLNVIVVRIPPLRERTEDFLPLIAHFLKKHGERLGKPDAAIAPEA
                     IEVMRRHTWPGNVREMENVIERALVLGRGPVIEMGDLPEALLKPAGDKESLPPLQSVA
                     LVEREHILKTLRAVAGNKAAAARLLGLDRKTLYRKLKQYHIQQG"
BASE COUNT          258 a          507 c          641 g          271 t
ORIGIN      
        1 cgggcggcgc ggtggtcgtg tgcggcgcgg tggtcgtcgg gaccgccgtc gtgtcgggag
       61 gtgcctgggc gtgcgcgggc gcggccgcga gtgccaggag gaggaggggg atgcaggcca
      121 ccttcatggg aggaagagta ggcattgacg agtccgcgcg ggggttcaat ctggtgatgc
      181 gatcagaaat cgtgtgaaac gagcgcgaat tccacgcggc ggcccgcccc atgtggggca
      241 aatagggaca catttcgatg ggtcacgccc ggacttgcga ctccgcggag acccgactcc
      301 ccactgccta cggctagaaa ttgacgaagt aacccgaggt ccccgcgatg tcgcgaagga
      361 ccgggagcac ctcgtgggga tcgtcggaca ggcgggccaa tcgagtcgat ccgcggagtc
      421 ctggatgggc ggaacgttgc cggagcatct cttccgtgac gcggaggaga tccccgaccc
      481 gggtaaggag gtagcaaccc gggctgttca actcgctcca gcgcacctcg gggagacgct
      541 gccggcggcg ttccgtgctg tccggttcgg tggaatcggc cgaagaatcg acgggtggtt
      601 cgggagtcac gcggccgcag gcctgcgaaa ggcgtgccag ccgggggtcc gccgggcgag
      661 gggtgccgca cggcaggccg gggcaccgcg ggtgggtagt gcgcgcccgg cggttacccc
      721 tgctggatgt ggtactgctt cagttttcga tacagggtct tgcggtcgag gcccaggagg
      781 cgggcggcgg cggccttgtt gccggccacg gcgcggagcg ttttcaaaat gtgctcgcgc
      841 tcgacgaggg ccacggactg gagcggcggg agcgattcct tgtcgccggc gggcttgagg
      901 agggcttcgg ggaggtctcc catctcgatg accgggccgc gaccgaggac gagcgcccgc
      961 tcgatgacgt tttccatctc gcggacattc cccggccagg tgtggcggcg catcacctcg
     1021 atggcttcgg gggcgatggc ggcatcgggc ttccccagcc gttcgccgtg cttcttgagg
     1081 aagtgggcga tgagcgggag gaagtcctcg gtgcgctcgc gcagcggcgg gatgcggacc
     1141 acgatgacat tgagtcgata atagagatcc tcgcggaacg acccctcctt cgcccgctgg
     1201 gcgaggtcgc gatgcgtggc ggccaccacg cgcacatcga ccggcacggg ctgggtagcg
     1261 cccacgggcg tcacctcctt ttcctggagc acgcgcagga gctttccctg gagcgcgggc
     1321 ggcatgtcac ccacttcgtc gaggaagatg gtgccgccgt tggcctcctg gaagaggccg
     1381 gggcggtcgt cgcgcgcgtc ggtgaacgaa ccgcgacgat ggccgaagag aaggctctcg
     1441 atcaggtttt cgggaatggc cgaacagttg accgcgagga agcggccggt acgccccggg
     1501 tgcccggcat ggagggcccg cgccaccagc tccttgccgg tgccgctttc gccggtgatg
     1561 agcacggatg ccttcgacgg tccgacctgc gcgacgaggt cgaggacggc gcgcatcttc
     1621 gcgtcttcgc tgacgatgtg gccgatggcg ttgcgctccc gcaaacgctc gcgaagc
//
LOCUS       DASVOD010000093         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_788730, whole genome shotgun sequence.
ACCESSION   DASVOD010000093 DASVOD010000000
VERSION     DASVOD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788730"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1569)
                     /locus_tag="VF720_01865"
     CDS             complement(139..1569)
                     /locus_tag="VF720_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7878124.1"
                     /translation="MRINGLTGFRGLLPVAFLGLSMHGCSDGGGGGGPQGGRPVIERI
                     VASPNVVPKGGASQFSALATDPDGDSLSYAWTVEAGTLSDNDLVRVGWTAPASAGIIE
                     VRVVVSAQDGADTAFANIAVGSGALRVESDPPGALVYLNASLRPGQTPLQFNNLAVGD
                     YNVQLAGLYFRYEPASIDAPVEDAETTVVSFHLPEARVEMVDTGPDAYDEMGGFTYTE
                     GGFGLVFSARIGNSAAVRAASLVSTHAGTNGRLLYPDATIGEPMSLKAVGFDPSLAFV
                     AGDDIYIGLLHDSNLDGLMETLTDVRRLNGTSGSAYAPAYNLAGTTLAFALTPSSTPN
                     TTDIMLEGDIDSAQVTRVRRVSARGGNYPTYRPDDSIVYESGGELYRVYVDSLVPALP
                     VKLTDTGGHARAPAASPDGDYIAYLDDRGLLQIFIPALGVTTTILEEVHAAHVKWAPN
                     SRELLIDDNPPGGPGRIKLVTELPFP"
     gene            complement(1566..>2434)
                     /locus_tag="VF720_01870"
     CDS             complement(1566..>2434)
                     /locus_tag="VF720_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEX7878125.1"
                     /translation="QQAARRLDQLESEKVRHPDETAPRRMAETLLALAEQVPRGVDRV
                     TLAAADNGEPLEIPLEPGRSAIENAERWFKEARKARQAAERLPKEIARASREVARWSA
                     FEERLASLAPDDAAGVAALAGELDGRSGARRGASSTSGTRDVTGGGGPSGGRRRDEPG
                     AAMQPRRYRLPGGWTILVGRSNAANDYLTHRLAEPHDLWFHAHGCPGSHVVLKGPDRR
                     AEPDRSVIEAAAALAALNSKARHASRVPVIYAEKRHVRKPRGSKPGLAAVTNEKSMMV
                     RPGEPAETVEDS"
BASE COUNT          356 a          844 c          843 g          391 t
ORIGIN      
        1 ggggcggccg cgagcgtcag gagcagggcg gccaggaaaa tgcggatcgg cttcatcctc
       61 gtctcctggg gcaggcaccg acaggtccgg cgccgcgccg cccggccgaa tgccaggcgg
      121 cagtccggtt caacaccatc aggggaacgg aagttcggta accagtttga tgcggccggg
      181 gcctccgggc ggattgtcgt cgatgaggag ctcccggctg ttcggggccc acttcacgtg
      241 ggcggcgtgc acctcctcca ggatcgtcgt ggtgacgccg agggccggga tgaagatctg
      301 cagcaggccc cggtcatcga ggtacgcgat gtagtccccg tcgggggatg cggccggtgc
      361 ccgcgcatgt cccccggtgt cggtcaattt taccggcagc gcgggaacga gcgaatcgac
      421 atagacccgg tagagctcgc cgcccgattc gtacacgatc gaatcgtccg gtcgataggt
      481 cgggtagttg ccgccccgtg ccgacacccg ccgcacccgc gtgacctggg cgctgtcgat
      541 atctccttcc agcatgatgt cggtggtgtt cggcgtgctc gacggggtca gtgcaaaggc
      601 cagcgtggtc cccgccaggt tgtacgccgg agcgtaggcc gagccgctgg taccgttcag
      661 gcggcgcacg tcggtgaggg tttccatgag gccatcgagg ttcgaatcat ggaggaggcc
      721 gatgtagatg tcgtcgcccg ccacgaaggc cagtgacggg tcgaatccga ccgctttcaa
      781 gctcatcggt tcgccgatcg tggcgtcggg atacaggagc cggccgttgg tgccggcgtg
      841 ggtggacacg aggctggcgg cccgcaccgc ggcgctgttc ccgatccgcg cgctgaagac
      901 gaggccgaac ccaccctcgg tgtaggtaaa gccccccatc tcgtcgtagg cgtccggtcc
      961 ggtgtcgacc atctcgacgc gcgcctccgg aaggtggaac gacaccaccg tcgtttcggc
     1021 gtcttccacg ggggcgtcga tcgatgccgg ttcgtagcgg aagtagagcc cggcgagctg
     1081 cacgttgtag tcgccgacgg ccaggttgtt gaactggagc ggcgtctgcc cggggcgaag
     1141 cgacgcattg agatacacga gagcgccggg aggatcgctc tccacgcgga gcgcgcccga
     1201 accgacggcg atgttggcga acgccgtgtc cgcgccgtcc tgggcgctca cgaccacgcg
     1261 cacctcgatg atcccggcgc tggccggcgc cgtccatccg acacggacga gatcgttgtc
     1321 cgagagggtg cccgcctcga ccgtccatgc gtagctgagc gaatcaccat cggggtcggt
     1381 ggccagggcg ctgaactggc tggcgccacc cttgggcacg acgttcgggg aggccacgat
     1441 ccgctcgatc accggccggc ccccctgcgg cccgccgcca cctccaccat cgctgcagcc
     1501 atgcatcgac aggccgagga acgcgacagg gagaagccca cgaaacccgg tcaggccatt
     1561 gatcctcacg agtcctccac cgtttccgcc ggctctcccg gccggaccat catcgacttc
     1621 tcgttcgtca cggcggccag gccgggcttt gaaccccggg gtttccgcac gtgccgcttc
     1681 tcagcataga tgaccggaac ccgggatgca tgccgggcct tgctgttcag cgccgccagg
     1741 gccgccgcgg cttcgatcac cgagcggtcg ggctcggcgc gcctgtccgg ccccttcagg
     1801 acgacgtgcg atccggggca gccatgcgcg tggaaccaga ggtcgtgcgg ctcggccaga
     1861 cgatgggtca ggtagtcatt ggcggcgttg ctccgtccga ccaggatggt ccagccgccc
     1921 ggcaggcggt agcgccgcgg ttgcatggcc gcgcccggct cgtcgcgccg gcgaccgccc
     1981 gatgggcccc cgcctcccgt aacatcgcgc gtgccggagg tggaggacgc gccgcgtcgc
     2041 gcgccgctac ggccatccag ttcccccgcc agggcggcca cgcctgccgc atcgtccgga
     2101 gcgagcgagg ccagccgctc ctcgaaggcg ctccatcgtg ccacctcccg gctggcccgg
     2161 gcgatctcct tcggaagacg ctccgccgcc tgccgggcct tgcgcgcttc cttgaaccag
     2221 cgctcggcat tctcgatggc cgaacgcccc ggctccaggg ggatctcgag cggctcgccg
     2281 ttatccgctg ccgcaagcgt cacccggtcc accccgcgtg gcacctgctc ggccagggcc
     2341 aggagtgtct cggccattcg acgcggggct gtttcatcgg gatggcgaac cttctcggat
     2401 tcgagttgat cgaggcgcct ggccgcctgt tgcc
//
LOCUS       DASVOD010000094         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_58402, whole genome shotgun sequence.
ACCESSION   DASVOD010000094 DASVOD010000000
VERSION     DASVOD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58402"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..>1924)
                     /locus_tag="VF720_01875"
     CDS             complement(62..>1924)
                     /locus_tag="VF720_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HEX7878126.1"
                     /translation="DGRLERGEITFESFPDRDEMDYRVTGHLAGVAASLPGKGRVIDA
                     GDADVELKPDRGHLAGNFRSGQATLGLNAVLTKFADPHWTANIELAGPLAEALRFLPP
                     QPDLEVNAGHVAAKLNAAGRFGPTTVPEVDGTVVLRGAALRHTSIAVPVDRFDCDIDL
                     NGRTVHLRDGVVQAGRSDARFTGRIDDYTKPKLDADIQAGTIDLAELFPATGGTATTA
                     EPRKGGASRPPVTGHITIDRMVRKEVTLTDVSSSFAIDANGMGLTNLTGHAWGGTVKG
                     NLQLSPKGDQELDYAGDLDIQGVHAEQLLATYTPIRGLQGLLTTDIKLSGRNAPGLNP
                     LTVLTLIGKGLVIEGSFVNLPIIRKVSEAVGYTPGMAATIPFKTIRHSIRVQNGFADL
                     DSFTIRQDAADWRVGGRIGLDGRLDCPVTARVAVNQFRPGTRLAEFAALLADKDGRLP
                     IAFHLGGTLTSPDVKLNLEPLLASAQKKAEQKIGDELRRRLEDLLRGRKPSGGGGTPP
                     GGTGTPTGGSGTPTGGTPQDTAATTPPGSTTPTPVPTPVPPKPSVEDELKKRLDELLG
                     KKPKAPPPPPPPRDTTVREPPVPPDTAAPQPPPPDTGSANPDTLSRSGSGRDST"
BASE COUNT          254 a          623 c          700 g          347 t
ORIGIN      
        1 atgttccgga acaggcattc acggagcatc ctggtgcgac atgccggggc ctgtcctccg
       61 atcaggtcga gtcccggccg gatccgctcc gcgacagcgt gtcgggattc gcactgccag
      121 tatcgggtgg cggcggctgg ggcgcggccg tgtcgggcgg caccgggggt tctcgaactg
      181 tggtgtcgcg cggcggagga ggcggcggcg gcgccttcgg tttcttgccc agcagttcgt
      241 cgaggcgctt cttcagctcg tcctcgacgc tcggcttggg cggcacgggt gtgggcaccg
      301 gtgtcggagt cgtcgagccc gggggcgtgg ttgccgccgt gtcttgcggt gttccccccg
      361 tcggcgtgcc ggatcccccc gtcggcgtgc ccgtcccacc cggaggtgtc ccgcccccgc
      421 ccgacggctt gcgtccccgc agcagatctt ccagccgccg gcgcagctca tcccctatct
      481 tctgctccgc cttcttctgc gccgaggcca ggagcggttc caggttcagc ttcacgtcgg
      541 ggctggtcag cgtgccgcca agatgaaacg ctatcggcag gcggccatcc ttgtcggcga
      601 gaagggcggc gaattcggcg agccgggtgc cggggcggaa ctggttcacg gccacgcggg
      661 cggtgacggg gcagtcgaga cggccgtcga gtccgatgcg gccgccgacg cgccagtcgg
      721 ccgcgtcctg ccggatggtg aacgagtcga ggtcggcaaa gccgttctgc acgcggatgg
      781 agtgacggat cgtcttgaac ggaatcgttg cggccatgcc gggcgtgtag cccaccgctt
      841 cggagacttt ccggatgatc ggaagattga cgaagctccc ctcgatgacg agccccttgc
      901 cgatgagcgt gaggaccgtg agcggattga ggcctggagc attccggccg gagagcttga
      961 tgtcggtggt aagcaatccc tggagaccgc ggatcggcgt gtatgtcgca aggagctgct
     1021 cggcgtggac cccctggatg tcgaggtcgc cggcgtagtc gagctcctgg tcccccttcg
     1081 gcgagagctg gaggtttccc ttcacggtgc cgccccaggc gtgcccggtg aggttggtga
     1141 gccccatgcc gttggcgtcg atggcgaagc tgctgctcac gtcggtgagc gtgacttcct
     1201 tccgcaccat gcggtcgatc gtgatgtggc cggtgaccgg cgggcgggag gcgccccctt
     1261 tcctcggctc ggccgtcgtg gcggtgccgc cggtggccgg gaagagctcg gccaggtcga
     1321 tggtgccggc ttggatgtcg gcgtcgagct tcggcttcgt gtagtcatcg atccggccgg
     1381 tgaagcgggc gtcgctcctg ccggcctgca cgactccatc gcgcaggtgc accgtgcgcc
     1441 cgttgaggtc gatgtcgcag tcgaatcgat caaccggcac cgcgatgctc gtatggcgca
     1501 gcgcggcccc tcgaagcacc acggtgccat ccacttcggg aacagtcgtg ggcccaaagc
     1561 ggccggccgc attgagcttt gcggcgacat gacccgcgtt cacttccagg tccggttgcg
     1621 gcggcaggaa gcggagcgct tcggccaatg gcccggccag ttcgatgttg gccgtccagt
     1681 gcggatcggc gaacttcgtg agtacggcat tgaggccgag cgtggcctgc cccgaccgga
     1741 aattgccggc caggtggccg cggtcgggct tcaactcgac gtcagcgtcc ccggcatcga
     1801 tgacccgacc cttccccggc agcgaggcgg ccacgccggc aaggtggccg gtgacgcggt
     1861 agtccatctc gtcgcgatcc gggaacgatt cgaaggtgat ctctccccgc tcgaggcggc
     1921 cgtc
//
LOCUS       DASVOD010000095         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_527902, whole genome shotgun sequence.
ACCESSION   DASVOD010000095 DASVOD010000000
VERSION     DASVOD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527902"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF720_01880"
     CDS             <1..640
                     /locus_tag="VF720_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7878127.1"
                     /translation="VNEADKVQVRLDRHTLLDATIVGTDPRSDLAVLKVDRKNLQALP
                     FGNSDNVKIGEWVVACGAPFGLERSITVGIVSAKGRSNVGVTDYEDFIQTDAAINRGN
                     SGGALINLKGELVGINTAIATQTGGSNGVGFSIPINMARYIMSSLIADGKVTRGWIGV
                     NISNVDENIVKGLGLPKDQTGVVISGVTDGSPADRAGLKSNDVIVKLDGHMI"
     gene            644..1099
                     /locus_tag="VF720_01885"
     CDS             644..1099
                     /locus_tag="VF720_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7878128.1"
                     /translation="MADLRNRVSTTKPGTTVDLVIMRDGSNRDVSVKLDELPSNLAEN
                     TGQEPREDEESPTEQGTIDRQLGVQVTTLTTEKARELEIKERRGVLVTAIAPNSPAAR
                     AGLRPDDLILEVNKAPVQTSDEFRSAVEKAGDEPVMRILRGDSYLFIVF"
     gene            1153..2400
                     /locus_tag="VF720_01890"
     CDS             1153..2400
                     /locus_tag="VF720_01890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7878129.1"
                     /translation="MERPANRASLKIAFFTDTWHPSHNGVVTSTTTFRQDMIDLGHQV
                     HVFAPAAPGLDPEPGVTRVASLPFPAEPNCRVALPFPRSLLVKFTRSRFDIIHTQTPF
                     GLGLWGAALARVARRPLVHTYHTFFAEYSHYLKVGEEFGRAFAVQYSRLYCNRCRAIV
                     VPSPEFIDVLRSYRITTRIEVIPTGLRLPERLPDKPEARRRLGLEPGTRQLLYVGRLA
                     REKSVAEVLDAVATLHRTDPTLRLALVGDGPFRDELEQQVERLGLGNVVRFVGGIPHE
                     EVWHWYAASDLFLFASQTETQGLVVAEALSAGLPVVAMRGPGVRDFVKPMNGGLLTEP
                     DAADFLAAIRRVLADDEGRATMGRLGRAAAAQWSSRYQAERLIELYRRLLRRPARRRI
                     LKRAMATVDRIGGRYRRRLSASA"
     gene            complement(2370..>3242)
                     /gene="prmC"
                     /locus_tag="VF720_01895"
     CDS             complement(2370..>3242)
                     /gene="prmC"
                     /locus_tag="VF720_01895"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX7878130.1"
                     /translation="VGAWLADATRRLRAHNPDAARDARLLVLSVLALPPAAILANPQR
                     PLSAEEVERLEELVAERLRGTPLQHLTGRAPFRNLELSVGPGVFVPRPETEGLVEHLL
                     EFIRKGGAGESPRILELCAGSGAIGLALVDEAPPSEVTAIERSETAAACARGNARRLL
                     GPERSHRWTLLGGDLYEPLSGRRMPNEFDVIVANPPYIPDGLWGTLPREVRVHESPLA
                     LLGGPDGTEVIARIVAGAPGWLKRGGLLAMEIDESHAERVLAILDDGGFENRRVASDL
                     AGRPRYALADNRRR"
BASE COUNT          519 a         1156 c         1094 g          473 t
ORIGIN      
        1 ggtgaacgag gccgacaagg tgcaggtgcg cctcgatcgc cacacgctcc tcgacgccac
       61 gatcgtcggg accgacccgc gcagcgacct ggcggtgctc aaggtcgatc gcaagaacct
      121 ccaggccctc cccttcggca attcggacaa cgtgaagatc ggcgaatggg tggtggcctg
      181 cggcgccccg ttcggcctcg agcgcagcat cacggtgggc atcgtcagcg ccaaggggcg
      241 cagcaacgtc ggcgttaccg actacgagga cttcatccag accgacgccg ccatcaaccg
      301 cggcaactcg ggcggcgcgc tcatcaacct gaagggtgag ctggtcggca tcaacaccgc
      361 catcgccacc cagacgggcg gttcgaacgg cgtcggcttc tcgatcccga tcaacatggc
      421 ccgctacatc atgagcagcc tgatcgcgga cggaaaggtc acgcgcggct ggatcggcgt
      481 gaacatctcg aacgtcgatg agaacatcgt gaagggtctc ggccttccga aagatcagac
      541 cggggtcgtc atctcgggcg tcaccgacgg cagtccggcc gaccgggcgg gcctcaagag
      601 caacgacgtc atcgtgaagc tcgacggcca catgatctag gatatggccg acctccggaa
      661 ccgcgtctcc acgaccaagc ccggcaccac ggtcgatctc gtgatcatgc gcgacggctc
      721 gaaccgcgac gtcagcgtca agctggatga gctgccgagc aacctggccg agaacacggg
      781 gcaggagccg cgtgaagacg aggagtcgcc gaccgagcag gggaccatcg accggcagct
      841 gggagtgcag gtgaccacgc tcaccaccga gaaggcccgc gagctcgaga tcaaggagcg
      901 ccgcggggtc ctggtgacgg cgatcgctcc gaacagcccg gccgcccggg ccggcctccg
      961 gcccgatgac ctgatcctcg aggtcaacaa ggcgccggtc cagacgtcgg acgagttccg
     1021 gtccgccgtg gagaaggccg gtgacgagcc ggtgatgcga atcctgaggg gcgacagcta
     1081 cctgttcatc gtcttctagg ccccttcgtc aatacacccc gggcggggcg ctcctgctag
     1141 aatgcacttc cgatggagcg tcccgccaac cgggcctcgc tgaagatcgc cttcttcacc
     1201 gacacctggc atccgagcca caacggcgtg gtcacctcga ccaccacctt ccgccaggac
     1261 atgatcgacc tgggccacca ggtgcatgtc ttcgccccgg ccgctcccgg actcgatccg
     1321 gaacccggcg tcacccgcgt ggcctcgctc cccttcccgg ccgagccgaa ctgccgcgtg
     1381 gcactcccct tcccccgctc gctcctcgtc aaattcaccc gctcgcgctt cgacatcatc
     1441 cacacccaga cgccgttcgg cctcgggctg tggggcgccg cgctcgcgcg ggtggcgcgt
     1501 cgaccgctgg tgcatacgta tcacacgttc ttcgccgagt attcgcacta cctcaaggtg
     1561 ggcgaggagt tcgggcgcgc gttcgccgtg cagtacagtc ggctctactg caaccgatgc
     1621 cgggcgatcg tcgtgccttc gccggagttc atcgacgtgc tccggtccta ccgcatcacg
     1681 acgcgcatcg aggtgatccc gactggcctt cgcctgccgg aacgcctccc cgacaagccg
     1741 gaggctcgac gccggctcgg cctcgaacct ggcaccaggc agcttctcta tgtggggcgg
     1801 ctggcacgcg agaagagcgt ggccgaggtc ctggacgcgg tggccacgct ccatcgcacc
     1861 gaccccaccc tccgcctggc gctggtgggt gatggcccct tccgggacga gctcgagcag
     1921 caggtcgagc gcctcgggct ggggaacgtc gtgcgcttcg tcggtggcat cccgcatgag
     1981 gaggtctggc actggtacgc cgcgtccgac ctcttcctct ttgcctcgca gacggaaacg
     2041 caggggttgg tggtggccga ggcgctctcg gccggcctgc ctgtcgtggc catgcggggc
     2101 ccgggcgtgc gcgacttcgt gaagccgatg aatggcggac tgctcaccga gcccgatgcg
     2161 gccgacttcc tggcggccat ccgccgcgtc ctggcggatg acgaagggcg ggccaccatg
     2221 ggacgcctcg gtcgcgcggc cgccgcccag tggagcagcc gctaccaggc cgagcgcctg
     2281 atcgagctct accggcggct cctccgccgc ccggcccgcc gtcgcatcct caaaagggcg
     2341 atggccaccg tggaccggat cggcgggcgt taccgccggc ggttgtcggc cagcgcgtag
     2401 cggggacgac cggccaggtc gctggccacc cggcggttct cgaaaccacc gtcatcgagg
     2461 atggccagca cccgctcggc atggctctcg tcgatctcca tggccaacag cccacccctc
     2521 ttcagccacc cgggcgctcc ggccacgatc ctcgcgatca cctcggtgcc gtccgggccg
     2581 ccaagcaggg ccaggggcga ttcgtgcacg cgcacctcgc gcggaagcgt cccccacaac
     2641 ccgtcgggga tgtacggcgg attggccacg atcacgtcga actcgttcgg catccggcga
     2701 cccgaaagtg gctcgtagag gtcgccgccg agcagtgtcc agcggtggga gcgctccggg
     2761 ccgagcagcc gccgggcgtt gccgcgggca caggccgcgg cggtttcgga gcgctcgatg
     2821 gcggttacct cggagggggg cgcctcgtcg accagcgcga gcccgatggc cccgctgccg
     2881 gcgcacagct cgagaatccg gggagactcg cccgcccccc ctttccggat gaactcgagg
     2941 aggtgctcca cgagcccctc ggtctccggc cggggaacga agacgccggg gcccaccgac
     3001 aactcgaggt tgcggaacgg cgcccttccg gtgagatgct ggagcggcgt gccgcgcagc
     3061 cgttcggcca ccagttcttc gagtcgctcg acctcctcgg ccgagagcgg acgctgcggg
     3121 ttggcgagga tggcggcggg gggaagcgcc aggaccgaca ggacgaggag tcgggcgtcg
     3181 cgggcggcgt cgggattgtg ggcgcgcagc cgccgcgtgg cgtcggccag ccaggcgccc
     3241 ac
//
LOCUS       DASVOD010000096         3534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_214864, whole genome shotgun sequence.
ACCESSION   DASVOD010000096 DASVOD010000000
VERSION     DASVOD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214864"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VF720_01900"
     CDS             complement(<1..1057)
                     /locus_tag="VF720_01900"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEX7878131.1"
                     /translation="MTNHLPPDTIVVDGHADTLLGIVDKGHHIDSAPSDGFHIDLPRM
                     AKGGLHAEIFTAFVDPQFIPGARERAHRLIDALHVEARTFPERMAITTSADAVREAVA
                     NGRVAAIPAIEGGHAIEDSLDNLREFARKGIRLMTLTWNNSNGWADGCWPHEGDVRHG
                     GLTDFGRRVIGEMEKLNVLVDVSHASPETFRDVAAVATRPFVASHSGAASVTPHFRNL
                     TDDQLDVIARVGGIVGVPFVSAFLVDELAVWKEIQASEAYAKLTRPTYYPFPYHAEED
                     EEELYEASVPQATLDDFFRHLDCIVKRIGWQHVALGTDFDGTRRMPSEIADVADLPKV
                     TAALRSRGLSEEAVRGIL"
     gene            1116..1361
                     /locus_tag="VF720_01905"
     CDS             1116..1361
                     /locus_tag="VF720_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002643428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878132.1"
                     /translation="MTDDRGRERTDRILGEMIIQQRAKVLRVARDHAPNATPEDILNP
                     HDIPELARAPIFHFEDGILSGLISAQIALRAKDPGDA"
     gene            1354..>3534
                     /locus_tag="VF720_01910"
     CDS             1354..>3534
                     /locus_tag="VF720_01910"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX7878133.1"
                     /translation="MRKHPGIVGVVVLFGLLGAFWRQDAGPARPDWAGTDDGKAALLD
                     FRRNEMTRYVRAGADPAEPRLVPADAPLPPYYPPADPMPPYDVHRYDIDLEIFPEDSI
                     AMGSTAISGEIMRDGLDSLVVDYCGPEVFSVELDGVPSDDWVRHGGKLAVALDPPRLF
                     HDDFVLQVFHGGVHDRGIYFPRDDQPTTLATHTFSEPEDARWWFPCHDVPNDKATLDM
                     VVTVPADHVVGSNGVLLEEIALEDGRRRFVWRETRPLATYLMTFQAAPYVLLHDDRVL
                     GVPIVNYVYPADSAEALVAFEPVPAMIDFFSQVFGPYPFDKYGHTEASFPGGMEHQSL
                     SMVGEFVVRNGQNYEWLIAHELAHQWFGDAVTLADWRHIWLNEGFASYGDVLWHEHRY
                     GREGLLARLRLFSDLFKLGYLQNGFSIPVYNPPAERLFNFAAYDKGAWVVHMLRGIVG
                     DENFFDILRTYQARFRYGNVVNDDFITICEEFYGGNLDWFFTPWLNDPGLPRYLVSSE
                     SAPLGDGRYDVSVSVVQTQLMLPQPGGLFPMPVTFALRSETGETRRTFFVDASPFAFR
                     DTIESAPLDTVAILDPDEWILKTVDYTTLVPVRVTGFAASTGADGSVLLRWRHDDGPR
                     DAQFRVWRENRAGEVDGLPGTGAAVLTPEWLSGRSSYEFLDVVPGVDAAWWIEARDRQ
                     GAVDWYGPVRATSVIPPSRLAVAVVPGADGAVTLSLTLPRAGHLQ"
BASE COUNT          570 a         1208 c         1147 g          609 t
ORIGIN      
        1 ccaggatccc ccgcaccgcc tcctccgaaa gcccgcgcga ccgcagcgcc gccgtcacct
       61 tcggcaagtc cgccacatcc gcgatttccg acggcatccg ccgcgtcccg tcgaaatccg
      121 ttcccagggc cacgtgctgc cacccgatcc gcttcacgat gcagtcgaga tgccgaaaga
      181 aatcgtccag cgtggcctgc ggcacggacg cctcgtacaa ttcctcctca tcctcttccg
      241 cgtgataggg gaaggggtag taggtcgggc gcgtgagctt ggcgtaggcc tccgaggcct
      301 ggatctcctt ccacaccgcc agttcatcga ccaggaacgc cgaaacgaac ggcacgccca
      361 cgatcccgcc caccctggcg atcacatcga gctggtcatc cgtcaggttc cggaaatgcg
      421 gcgtcaccga cgccgcaccc gaatgactgg ccacgaaggg cctggtggcg acggcggcca
      481 cgtcgcggaa cgtctcgggt gaggcatgtg atacgtccac cagcacgttc agcttttcca
      541 tttcgccgat gacacgcctg ccgaagtcgg tcagcccacc atgtcgcaca tccccttcgt
      601 ggggccagca gccgtcagcc cagccgttgg agttgttcca ggtcagcgtc atcaggcgga
      661 tgcccttgcg ggcaaattcc cggaggttgt cgaggctgtc ctcgatggca tggccacctt
      721 cgatggcggg aatggcggcc accctgccgt tggccacggc ctcgcggacg gcatcggcgc
      781 tggtggtaat ggccatgcgt tcgggaaagg tgcgggcctc gacgtgcaga gcatcgatca
      841 gccggtgcgc ccgttcccgg gccccgggga tgaactgggg atcgacgaaa gcggtgaaga
      901 tctccgcgtg caggcccccc ttcgccattc ggggcaggtc gatgtggaat ccgtcggagg
      961 gggcgctgtc gatgtgatgg cctttgtcga cgatgccgag gagcgtgtcc gcgtggccgt
     1021 cgacgacgat cgtgtcgggg gggaggtgat tggtcatgcg ggcattgtac aatcgacggg
     1081 ccgtcgggtc ggggtcccga agggcgggag atctcatgac cgacgacagg ggccgggagc
     1141 gcacggatcg catcctgggc gaaatgatca ttcagcagcg cgccaaggtg ctgcgcgtgg
     1201 cccgcgatca cgcccccaac gccacgcccg aggacatcct gaacccccac gacatccccg
     1261 aactggcccg ggcgccgatt ttccactttg aagacggcat cctgtcggga ttgatcagcg
     1321 cgcagatcgc gctacgggcg aaggatccgg gcgatgcgta agcacccggg gattgtcggc
     1381 gtggttgtgc tcttcggcct gctgggcgcc ttctggcgcc aggacgccgg cccggcacgg
     1441 cccgattggg ccggaaccga cgacggaaag gccgccctcc tggatttccg ccggaatgaa
     1501 atgacccgct acgtgcgcgc cggcgccgat cccgccgagc cacgactcgt gccggccgac
     1561 gcgccactgc cgccttacta tccgccggcc gatccgatgc cgccgtacga tgtccaccgc
     1621 tacgacatcg acctcgagat ctttcccgag gacagcatcg ccatgggttc caccgccatt
     1681 tcgggggaga tcatgcgaga cgggctcgac agcctggtcg tcgattattg cgggccggaa
     1741 gtcttctcgg tagagctcga cggcgtgccc agcgatgact gggttcgcca tggcggcaaa
     1801 ctagccgtgg cactcgatcc gccgcggctc ttccacgacg acttcgtgct ccaggtgttc
     1861 cacggcggcg tgcacgaccg cggcatctac ttcccgcggg acgaccagcc caccacgctc
     1921 gcaacccaca ccttcagcga gcccgaagac gcccgctggt ggtttccctg ccacgacgtg
     1981 ccgaacgaca aggccacgct cgacatggtc gtgacggtgc cggccgatca cgtggtgggg
     2041 agcaacggcg tgctcctcga ggaaattgcg ctggaggatg gtcgacgtcg cttcgtctgg
     2101 cgggaaacga gaccccttgc cacctacctc atgacgttcc aggcggcccc ctacgtcctc
     2161 cttcatgacg atcgcgtgct cggagtgccg atcgtgaatt acgtctaccc ggcggattcg
     2221 gcggaagcgc tcgtggcttt cgaaccggtg ccggcgatga tcgacttctt cagccaggtg
     2281 ttcgggccct atcccttcga caagtacggc cacacggagg ccagcttccc cggcggcatg
     2341 gagcaccagt cgctctcgat ggtcggggag ttcgtcgtga ggaacggcca gaactacgaa
     2401 tggctgatcg cccacgaact cgcgcaccag tggttcggcg acgcggtcac gctggccgac
     2461 tggcggcaca tctggctcaa cgaggggttc gcgagctacg gcgacgtgct ctggcacgag
     2521 caccggtacg gccgcgaggg actgctggcc cgtctccgcc tcttcagcga tctgttcaag
     2581 ctcggctacc tgcagaacgg cttcagcatc ccggtctaca acccgccggc ggagcgcctc
     2641 ttcaactttg ccgcgtacga caagggggcg tgggtcgtcc acatgctgcg cggcatcgtg
     2701 ggggacgaaa acttcttcga catcctgcgc acctaccagg cgcgtttccg ctacggcaat
     2761 gtggtcaacg atgacttcat cacgatctgc gaggagtttt acggcggaaa cctcgactgg
     2821 ttcttcacgc cgtggctcaa cgatcccggc ctgccccggt acctggtgag ctcggaatcg
     2881 gcgcccttgg gtgacggtcg gtacgacgtg tccgtgtccg tcgtgcagac gcagctcatg
     2941 ttgccgcagc ccggcggcct gttcccgatg cccgtaacct tcgccctgcg cagcgagacg
     3001 ggggaaacgc gacggacctt cttcgtggat gcctcgccct tcgcgttccg cgacaccatc
     3061 gagtcggcgc cgctcgacac ggtggcgatc ctcgatcccg acgagtggat attgaagacg
     3121 gtggactaca cgacgctcgt cccggttcgc gtcaccggct tcgcggccag cacgggagcc
     3181 gacggcagcg tccttctccg gtggcgccac gacgacggcc cgcgcgatgc gcagttccgc
     3241 gtctggcggg aaaaccgcgc cggcgaggtc gacggcctgc cgggtacggg cgcggcggtc
     3301 ctgacgccgg agtggctgtc gggccgctcc tcctacgagt tcctcgacgt cgttcccggc
     3361 gtcgacgccg cctggtggat cgaagcgcgc gaccgacagg gagccgtcga ctggtacggc
     3421 ccggtccgtg ccacgtccgt catccccccg tcaaggctcg cagtggcggt ggtgcccggc
     3481 gcggacgggg ccgtcacgct ctccctcacg ttgccgcgcg ccggccatct ccag
//
LOCUS       DASVOD010000097         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_267247, whole genome shotgun sequence.
ACCESSION   DASVOD010000097 DASVOD010000000
VERSION     DASVOD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267247"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1927
                     /gene="nusA"
                     /locus_tag="VF720_01915"
     CDS             269..1927
                     /gene="nusA"
                     /locus_tag="VF720_01915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX7878134.1"
                     /translation="MNYEIIEALGQIAREKSVDRKLLIETLEQGLLSAARKKFGLNAQ
                     VGVRFDEGRGGIEMWLTKSVAETIADPALEMTLRDAQAVDPNATVGQEVMIPLDIAEF
                     GRNAIMAAKQVLVQRVREAEREMVYDEYVDKVGSLVRGIVQQVDRGSVFVKINLTEAV
                     IPARELISRDRFRQRDHVRAYVLSVDKQSKGPQVILSRTHPDFLVKLFEAEVPEIAEG
                     IVEIKAVAREAGHRSKIAVYSRDDRIDAVGACVGIKGSRVQSIVRELGGERIDIVPWS
                     SDPALFVSRALNPAKVLQASATPEAEKRVTVVVVDDQLSLAIGKGGQNARLAARLTGW
                     HIDLISQSENEARKKAGVIDLPVEELEGVTPKMAQKLVQSGIETAREVEKAGVAGLTA
                     VEGVGEKTAEKLLATAIAALAALAGAGAAAAGAPEAKVTEAGEPSDPTPVAEAISGTV
                     EVSESAPAPPEADGDEAQNPGVHSESMMESMEMKPEGEALLRGRGDDENKLWDDPDED
                     EEDGEEEEEDGDDDDDDLDDEDEDFEEDDLDDLDIDLDDDDDDE"
     gene            2079..>2276
                     /locus_tag="VF720_01920"
     CDS             2079..>2276
                     /locus_tag="VF720_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7878135.1"
                     /translation="MTKLRVYQVAKDFQISSEALLEILRGLNVEVKSHMSTVEDATIA
                     QIRQKFQKEQEAVKAEDARKRE"
BASE COUNT          463 a          677 c          813 g          323 t
ORIGIN      
        1 ccacccgcga accgatcgag ggacgatccc accacctggg ggtcatcacg ggtgtcgaag
       61 acgacatcct cctcctcgaa cagcccgatg tcgggccgac gcggatcccc atcgacgaaa
      121 tacgcaaggc acagttgaag cgggacccgt ggggggcggc caggggccgt cgctagggtt
      181 tcgaaggctc ggcgctccag gaacggcccc gctttcgggc ggaaggcggg ggccgggcta
      241 tcgccattgg cgaaaggatg ttgatccggt gaactacgaa atcatcgagg ccctcgggca
      301 gatcgcccgg gaaaagagcg tggaccggaa gctcctgatc gagactctcg agcaggggct
      361 cctgtcggcg gcccggaaga agttcggcct gaatgcccag gtcggagtcc ggttcgacga
      421 agggcgcggc ggcatcgaga tgtggctcac caagtcggtc gccgaaacca tcgccgaccc
      481 ggcactcgag atgacgcttc gtgacgcaca ggccgtcgat cccaacgcca cggtcggcca
      541 ggaagtgatg atcccgctcg acatcgcgga attcgggcgc aacgccatca tggcggccaa
      601 gcaggtcctg gtgcagcgag ttcgcgaggc ggagcgtgaa atggtgtacg acgaatacgt
      661 ggacaaggtc ggctcgctcg tccgcggcat cgtgcagcag gtcgaccggg gaagcgtctt
      721 cgtcaagatc aacctgacgg aggccgtcat cccggcgcgc gaactgatca gccgggaccg
      781 tttccgccag cgcgatcacg tgcgggccta cgtcctttcg gtcgacaagc agagcaaggg
      841 gccgcaggtc atcctctcgc gcacccatcc cgatttcctg gtgaagctgt tcgaggcgga
      901 agtgcccgaa atcgccgagg gaatcgtcga gatcaaggcc gtggcccggg aggccggcca
      961 tcgctccaag atcgccgtct attcgcggga cgaccggatc gacgcggtcg gtgcgtgcgt
     1021 cggcatcaag ggttcgcgcg tgcagtcgat cgtgcgggag ctggggggcg agcggatcga
     1081 catcgttccc tggagttccg atccggcgct cttcgtcagc cgtgccttga acccggccaa
     1141 ggtcctgcag gccagtgcca ctcccgaggc cgagaagcgg gtgaccgtgg tggtcgtcga
     1201 tgaccagctc tccctggcca tcggcaaggg ggggcagaat gcccgcctgg ccgcccgact
     1261 taccggctgg catatcgacc tgatcagcca gtcggagaac gaggcccgca agaaggccgg
     1321 cgtcatcgac cttcctgttg aggaactcga aggggtgacg cccaagatgg cccagaagct
     1381 ggtgcagtcg ggcatcgaga cggcccgcga ggttgagaag gccggcgtgg ccggcctgac
     1441 cgccgtcgag ggagtgggag agaagacggc cgagaagttg ctggccacgg ccatagcggc
     1501 ccttgccgcg ctggccggcg ccggggctgc cgccgccgga gccccggagg caaaagtgac
     1561 cgaagccggg gaaccgtcgg acccgacgcc cgtggcggag gcaatttcag gtactgtcga
     1621 ggtatcggag tcggcaccgg ccccccccga ggcggacggt gacgaagcgc aaaaccctgg
     1681 agtgcattcg gagtcgatga tggaatccat ggagatgaaa ccagaaggtg aggcgctcct
     1741 gcgcggtcgc ggagacgacg agaacaagct ctgggacgat cccgacgagg atgaggaaga
     1801 cggggaggaa gaggaagaag acggggacga tgatgatgac gatctcgacg acgaggacga
     1861 ggacttcgag gaagacgatc tcgacgacct cgacatcgat ctcgacgacg atgatgacga
     1921 cgagtaggcg gtgatccggg ggcgcctcgc gccccggccg tgaaccggcc gctccctcga
     1981 atgggatcgg gccggcgcgg atcatcgatg agtcatcgac gtcgggccgg aatgccgcgg
     2041 tggcggattc cggcccacgt ctgaaagaga ggacggcttt gacgaaactc cgggtctacc
     2101 aggtagccaa ggacttccag atttccagcg aggcgctgct ggagatcctg cgcgggctca
     2161 acgtggaagt taaaagccac atgagcaccg tggaggacgc gaccatcgcc cagatccgcc
     2221 agaagttcca gaaggaacag gaggcggtga aagccgagga cgcgcggaag agggag
//
LOCUS       DASVOD010000098         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_528171, whole genome shotgun sequence.
ACCESSION   DASVOD010000098 DASVOD010000000
VERSION     DASVOD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528171"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VF720_01925"
     CDS             <1..700
                     /locus_tag="VF720_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878136.1"
                     /translation="ALLLGPVVPNVSRAGECCSSPDNGSGTATLPPASGGCYYYGSTE
                     IVDGLPGGDTIQINGYIGFFSAVSEIPGGTLSGTTSQGFNAVFFMSMTGTGSITGFSR
                     NINIPLVNERIDWGPRVPYAVTQSATAQYMALQGQITSDLDFDLLRVTGGDLFGMPAP
                     GAIELNSFAGGWLVSGFFSLWHRIDFVGSPISAYLAGRSGSTTRQRPFTLCNFQPVPV
                     EPSTWSNIKGLLAD"
     gene            822..1226
                     /locus_tag="VF720_01930"
     CDS             822..1226
                     /locus_tag="VF720_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878137.1"
                     /translation="MARITGVGGVFFKSKGDPKELAAWYQKHLGLPLEPWGGAALKWP
                     EDTAEDKGVTAWMLAKSDSKWFAPSEASFMINYRVDDMAGLLANLKSAGVEVLQGPES
                     HENGKFAWIVDPDGNKVELWEPKIFDEKNKES"
     gene            1301..1657
                     /locus_tag="VF720_01935"
     CDS             1301..1657
                     /locus_tag="VF720_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878138.1"
                     /translation="MIRIVMTVLALSLAAASPATAGRIFGDIKMDGKPAPAGLQVVIT
                     QPLAGKDSTAAGADTTVTDKFGSYKLNVKTEGKCLLSVKLAKETATLAVFSYKVATRY
                     DLILEKIEGKLSLRRK"
     gene            1665..2624
                     /locus_tag="VF720_01940"
     CDS             1665..2624
                     /locus_tag="VF720_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878139.1"
                     /translation="MNDNLEAPAEACPPEEITVVTARKQRDTWDKLQIILQPVGGLFT
                     ALAIAILGIVSSQFLERRQAAESNARLYSELMSRREEAESALRKDMFQTIIGAFVSPS
                     SHGDLNSELLNLELLAYNFHESLNLKPLFVDVRRRIAEGEQKATTAQARGEFDAYTER
                     IERVAREIARKQMIVLEGVGQKFDRTIDMTTDPVGTTLEPATLRLGSRQSSFTIDILD
                     LNRDRGEVLIGLSVDTPDSALGRQTKNATFTVGYFDFPMIDNTRLTGGDRCAVVLNSM
                     TDQSADMTLVLFPGAYASLKEKPYYSEVIESIRQANAGSAKAR"
     gene            complement(2638..3252)
                     /locus_tag="VF720_01945"
     CDS             complement(2638..3252)
                     /locus_tag="VF720_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878140.1"
                     /translation="MDRTFSKLAATLAPALLVGSLAGASFAADPASPDSAAGKPDTLQ
                     VQAAPPPSRSARQPSRIYYGGSAVVGFGNPDHVGFFPSLGYKLSPRVSIGGEIGYEYL
                     KYDGVKNGFNNYGGSLFSRVKLTPRLFGHAEYQAISTELATSPLSDNERVVVPYLLVG
                     GGMIQPISANASAYAEVLFDVLQDENSPYDSGEPFISFGVGVGF"
BASE COUNT          634 a         1072 c         1103 g          558 t
ORIGIN      
        1 ggcattgctg ctcggccccg tggtgccgaa cgtgtcccgc gcaggcgagt gctgttcctc
       61 gccggacaac ggttccggaa cggccacgct tccgccggct tcgggcggtt gctactacta
      121 cggttccacg gaaatcgtcg acgggctgcc cggtggcgac acgatccaga tcaacggcta
      181 catcggcttc ttctcagccg tgtcggagat tccgggcgga acgctctcgg gcacgacgag
      241 ccagggtttc aatgccgtct tcttcatgtc gatgacgggc accggcagca tcaccggctt
      301 cagccgcaac atcaacattc ccctcgtcaa cgagcggatc gattggggtc cgcgggttcc
      361 ctacgcggtc acccagtcgg ccacggccca gtacatggcc ctgcaggggc agatcaccag
      421 cgatctggat tttgacctgc tgcgcgtcac gggtggcgac ctgttcggca tgccggcacc
      481 cggtgccatc gagctgaatt ccttcgccgg tggctggctg gtctcgggct ttttcagcct
      541 ctggcaccgg atcgacttcg tcggcagtcc gataagcgcg tacctggccg gccggtcggg
      601 aagcaccacg cgccagcgcc cgttcacgct gtgcaacttc cagccggtgc cggtcgagcc
      661 aagtacgtgg tccaacatca aggggctcct ggccgactga acgaagtgcg aaaggtgcgc
      721 atcgtttgct gatccgtcgc gagctcaagc caggtatatg atgtgcggag gccccgcccg
      781 gttgggcggg gccttcacga ttgagtgaag aaggaggggc catggcacga atcaccgggg
      841 tcggcggcgt gttcttcaag agcaagggcg atccgaaaga gctggcggcg tggtaccaga
      901 agcacctggg gttgccgctc gaaccctggg gcggggccgc gctgaaatgg cccgaggaca
      961 ccgccgaaga caagggtgtt accgcctgga tgctcgcgaa gtccgacagc aagtggttcg
     1021 cgcccagcga ggcctcgttc atgatcaact accgcgtcga cgacatggca ggcctcctcg
     1081 cgaatctgaa atcggccggc gtcgaggtgc tccagggacc cgaatcgcat gagaacggca
     1141 agttcgcctg gatcgtcgac ccggacggca acaaggtcga gttgtgggag ccgaagatct
     1201 tcgacgagaa gaacaaggag agttgatccg ggaatcgggt ggagatgccc ggtgccctcg
     1261 gatatgattc tgcccgcctg gccggaaaga gaggagtgac gtgattcgta tcgtgatgac
     1321 ggtgctcgcc ctgtccctcg ccgccgcgtc gccggccacg gcggggcgca tcttcggcga
     1381 catcaagatg gatggaaagc cggctccggc ggggctccag gtggtcatca cccagccgct
     1441 ggccggaaag gactcgacgg ccgccggggc cgacacgacg gtcaccgaca agttcgggtc
     1501 ttacaagctg aacgtgaaga cggaagggaa gtgcctcctt tccgtgaagc tggccaaaga
     1561 aacggccacg ctggccgtct tttcgtacaa ggtggccacg cgctacgacc tgatcctcga
     1621 gaagatcgag gggaagctga gcctgcgtcg caagtgagcg cgcggtgaac gataacctcg
     1681 aagccccggc cgaggcgtgt ccgcccgagg aaatcaccgt cgtcacggct cgcaagcagc
     1741 gtgacacgtg ggacaagctc cagatcatcc ttcagccggt cggcggactg ttcaccgcac
     1801 tggccatcgc catcctcggc atcgtttcct cccagttcct cgagcgtcgg caggcggccg
     1861 aatccaacgc ccgcctctac tcggagctga tgagccggcg cgaggaagcc gagagcgcgc
     1921 tccgcaagga catgttccag accatcatcg gcgccttcgt gagcccgtca tcgcacggcg
     1981 acctcaactc cgaactgctc aacctcgagc tcctggccta caacttccac gagtcgctca
     2041 acctgaagcc gctcttcgtg gacgtgcgcc ggcggatcgc ggaaggggag cagaaggcca
     2101 ccaccgccca ggcgcggggc gaattcgatg cctacaccga gcgtatcgag cgggtggccc
     2161 gggaaatcgc gagaaagcag atgatcgtgc tcgagggagt ggggcagaag ttcgatcgca
     2221 ccatcgacat gacgaccgat ccggtcggaa cgacgctcga gccggctacg ctccggctcg
     2281 ggtcacgcca gtcgagcttc acgatcgaca tcctcgacct gaaccgggac aggggagagg
     2341 tcctcatcgg cctgtcggtc gatacgccgg attccgcact cgggcgccag acgaagaacg
     2401 ccacgttcac cgtcggctac ttcgacttcc cgatgatcga caacacgcgt ctgaccggcg
     2461 gcgatcgctg cgccgtggtg ctcaacagca tgaccgatca gtcggcggac atgacgctcg
     2521 ttctctttcc gggcgcgtac gccagcctca aggaaaaacc ctactacagc gaagtgatcg
     2581 agagcatccg gcaggccaac gcggggagtg cgaaggcgcg gtaacggcgc gctcccgtca
     2641 gaaccccacg cccacgccga aactgatgaa cggctcgccc gaatcgtacg gcgagttctc
     2701 gtcctgcagc acgtcgaaga gcacctcggc ataggccgac gcgttggcgc tgatcggctg
     2761 gatcatgccg ccccccacca ggaggtaggg aacgacgacc cgctcgttgt cactgagggg
     2821 gctggtggca agctcggtgc tgatggcctg gtattcggcg tgaccgaaca ggcgcggcgt
     2881 gagcttcacg cggctgaaga ggctgccgcc gtagttgttg aagccgttct tcacgccgtc
     2941 gtacttgagg tactcgtagc cgatttcgcc cccgatcgac acacgggggc tcagcttgta
     3001 gccgaggctc gggaagaagc cgacatggtc cggattgccg aaaccgacga cggccgaacc
     3061 gccgtaatag atgcgcgacg gttgcctggc cgatctggac ggcggcggtg cggcctgcac
     3121 ctggagagta tccggcttgc ccgccgccga atcgggagat gcgggatcgg cggcgaacga
     3181 ggcaccggca aggctcccca ccaggagagc cggcgccagt gtcgcggcga gcttcgagaa
     3241 tgtgcggtcc atagggcctc cccgggacgt tggattccgg gagagtaacc cctgccggtc
     3301 gggaagtccg ccgaaaagtc ccccatgccc tctctctcgc gaacgacctc ctgccgtttc
     3361 gtggggc
//
LOCUS       DASVOD010000099         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_841520, whole genome shotgun sequence.
ACCESSION   DASVOD010000099 DASVOD010000000
VERSION     DASVOD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841520"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VF720_01950"
     CDS             complement(<1..797)
                     /locus_tag="VF720_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878141.1"
                     /translation="MIRRLGSLVLILTVSTSAARGAVVPTASPFTTPTVSGRHIVMAQ
                     IGGSGPRPKDDPEKPLSDVPQVVNPKTAMLYSLLLPGLGQQAAGRGERARVFYAIEAG
                     IWTSFAIFRVQGDQRQDRYIEYAEFTAGVDASGKDDDYWRTLAQYERSDPGPASANEF
                     VRRQARALYPGDIVAQQQYFADNGYFENDTWDWQNSDNLARYQSLRSSSLDSYDKAEL
                     SIFAAIANRVVSVIDAARVAGQANREAAESSRSKDSGGGIGNPLNHLG"
     gene            complement(809..1630)
                     /locus_tag="VF720_01955"
     CDS             complement(809..1630)
                     /locus_tag="VF720_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878142.1"
                     /translation="MTATRFRRTASMPLGLPLLALALLAGPARALVLEPTLRVGMERP
                     LLVEDMLAAEPSSPEPGAAANLEPGPPEVPGVDGKRSPLLAGFLSLVVPGAGQFYNGS
                     RSGWLFLGLETAGWITYASARDTGHDIEEQYKRYADGHWTFERYRDPTFEDCPPEGHS
                     DGGVQDSTLVFLYETRRDDYYEDIGKLDIYSCGWDSQANREIYREMRNDSNDFLRLSR
                     TAGTMIFLNHLVSAVLAARGAAKHNARLPGGAELGFHMTPSITDPGATLIVSRRF"
BASE COUNT          280 a          607 c          633 g          292 t
ORIGIN      
        1 ccgaggtgat tgagcgggtt gccgatgccg ccgcccgaat ccttgctccg cgagctttcc
       61 gcggcttccc gattggcctg cccggccacg cgggcggcat cgatcaccga aacgacccgg
      121 ttggcgatgg ccgcgaagat cgagagctcg gccttgtcgt acgagtcgag gctgctgctg
      181 cggagcgact ggtagcgggc caggttgtcc gagttctgcc agtcccaggt gtcattctcg
      241 aagtagccgt tgtcggcgaa atactgctgc tgggccacga tgtccccggg atagagggcg
      301 cgcgcctgcc gccgcacgaa ctcgttcgcg ctggccgggc ccgggtcgct ccgctcgtac
      361 tgcgccagcg tgcgccagta gtcgtcgtcc ttcccggagg cgtcgacgcc ggcggtgaac
      421 tcggcgtact cgatgtaacg gtcctggcgc tggtccccct gcacgcggaa gatggcgaac
      481 gaggtccaga tgcccgcctc gatggcgtag aacacgcgcg cccgttcgcc ccggccggcc
      541 gcctgctggc cgagcccggg caggaggagc gagtagagca tggccgtctt gggattcacg
      601 acctgcggca cgtcggagag cggcttctcc ggatcgtcct tggggcgggg gcccgagccg
      661 ccgatctgcg ccatgacgat gtgccggccc gagacggtgg gcgtcgtgaa cggcgacgcc
      721 gtcgggacaa cggcgccccg ggccgccgag gtcgaaacgg tcaggatgag aacgagtgag
      781 ccgaggcgcc ggatcatgat gtctcctgtc agaaacgccg gctcacgatg agcgtcgcgc
      841 cggggtcggt tatcgagggg gtcatatgga atccgagctc cgcaccgccc gggagcctgg
      901 cgttgtgctt cgcggcgccg cgtgccgcca ggaccgcgct caccaggtga ttcaggaaga
      961 tcatcgtccc ggccgttcgc gagagccgga ggaagtcgtt cgagtcgttg cgcatctcgc
     1021 gatagatctc ccggttcgcc tgtgaatccc agccgcacga atagatgtcg agcttcccga
     1081 tgtcttcgta gtagtcgtcg cgccgggtct cgtacaggaa caccagggtc gagtcctgca
     1141 ctccgccgtc ggagtgacct tcagggggac agtcctcgaa cgtcggatct cggtaacgct
     1201 cgaacgtcca gtgcccgtcg gcgtaccgct tgtactgctc ctcgatgtcg tggccggtat
     1261 cccgggcgct ggcataggtg atccacccgg cggtctcgag gcccaggaac agccatccgc
     1321 tccgactacc attgtagaac tgacccgccc ccgggaccac cagggaaagg aagccggcca
     1381 ggagcggcga gcgttttcca tcgacgccgg gcacctccgg cgggcctggt tcgaggttgg
     1441 ccgccgcgcc cggttcgggg ctggacggct ccgcggccag catgtcttcg acgaggagcg
     1501 gccgctccat tcccacgcgg agcgtcggct cgagcaccag ggcccgggcc ggcccggcga
     1561 ggagcgcgag ggccaggagg ggaaggccga gaggcatgga ggccgtccgc cggaaacgag
     1621 tcgccgtcat gccgcttctt cctcctatcg ctaccgaagc accgagacga gccggacctg
     1681 gctccccggc gcgccggggc cctccacgtc gacccggacg agatagacac ccggggccac
     1741 gtctcccagg tgccagatga tgttggcctc acggccatca tttgaaggct cgagccccgc
     1801 cgccagcgga tg
//
LOCUS       DASVOD010000100         3310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_7420, whole genome shotgun sequence.
ACCESSION   DASVOD010000100 DASVOD010000000
VERSION     DASVOD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7420"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..1321
                     /gene="eno"
                     /locus_tag="VF720_01960"
     CDS             35..1321
                     /gene="eno"
                     /locus_tag="VF720_01960"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEX7878143.1"
                     /translation="MTTIEFVYAREILDSRGNPTIEVDVHLHGGTMGRAAVPSGASTG
                     VHEALELRDGETGRYLGKGVQQAVANVIETIGTELAGEDALDQALIDEMLLDLDGTDN
                     KSKLGANAMLGVSLAVAKAAAQALELSLYRYLGGPTARTLPVPMMNVINGGAHADNNV
                     DFQEFMIVPHRADSFCEGLRMGVETFHALKKTLHDRKLSTAVGDEGGFAPDLKSNSEA
                     LELLMTAIERAGYKPGDDISIALDLASSEFFKGGKYILAGDNNLTLDSAGMVEQLAKL
                     CDKFPIVSIEDGLAEEDWEGWKLLTDRLGKRCQLVGDDLFVTNTKILKRGIDTGVANS
                     ILIKVNQIGTLTETLAAVDMAKRAGYSAVMSHRSGETEDVTIADLAVATGVGQIKTGS
                     ASRSDRVAKYNQLLRIEEELGDAARYPGLSLFSGRK"
     gene            1370..1819
                     /locus_tag="VF720_01965"
     CDS             1370..1819
                     /locus_tag="VF720_01965"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEX7878144.1"
                     /translation="MNRTSLNEQVTNRYQRIFHDGYDNAAQHWKKGLALLLVTVTGLC
                     AWELVAGNQGILARKRVKADIARLIAANADLARQQKDYAAQNRGLATNDFMAEKAARE
                     ILHRARPGEIIYLFEESAAGGTPMTFSDVTIVQAPPAKADKEKPATR"
     gene            1873..1949
                     /locus_tag="VF720_01970"
     tRNA            1873..1949
                     /locus_tag="VF720_01970"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1907..1909,aa:Met,seq:cat)
     gene            complement(2108..>3310)
                     /locus_tag="VF720_01975"
     CDS             complement(2108..>3310)
                     /locus_tag="VF720_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878145.1"
                     /translation="NVAALVEGSVRKTGNRVRISVQLVKVADGFHLWSESYDRTLDDI
                     FAVQDDIAQAVVKELRTTLLGEAADSNASGQARAEVAVAGRGRGENGEAHRLYLQGRY
                     HADRLTAEDNVRAIEILKQVVAMEPGHALAWAQLSWAHTVSALKGFANVLDSNSAALD
                     AAQRAITLDPELAQAHRARGTVLLWSMWDWPRAEASLRKAVELAPENSDALNGLAMAL
                     LVTGRFDEALVFSRKAVELDPLAAIGYSVQGRILRAADRYAEAEIAIRKCIELEPNVV
                     SDRMVLSNILITLGRPDEAMAELANEPSDWGRLTGLALHHRHTGRLDDARAALEELIR
                     ISASHSAFQIAEIYADFGDADSAFEWLERACMQRDTGVALVMIDPLLRPLHEDPRWGA
                     FLEKMRLG"
BASE COUNT          622 a         1118 c         1063 g          507 t
ORIGIN      
        1 cgcgtcgagg aatcaaggcc ttcgggagtc gtccatgacc accatcgaat tcgtgtacgc
       61 ccgtgaaatc ctcgacagcc gcggaaatcc caccatcgag gtcgatgtcc acctgcacgg
      121 cggcaccatg gggcgggcgg ccgtgccttc gggagcctct accggggtcc acgaagccct
      181 ggaactgcgg gacggcgaga ccggccgcta cctcggcaag ggcgtccagc aggccgtggc
      241 aaacgtcatc gagacgatcg gcactgaact ggccggggaa gatgccctcg accaggccct
      301 gatcgacgag atgctcctcg acctggacgg caccgacaac aagtcgaaac tgggtgccaa
      361 cgccatgctc ggcgtctccc tggccgtggc caaggcggcc gcccaggccc tcgaactctc
      421 cctgtaccgc tacctgggcg gcccgaccgc ccggacgctc cccgtcccga tgatgaacgt
      481 catcaacggc ggcgcccacg cggacaacaa cgtggacttc caggaattca tgatcgtccc
      541 ccaccgggcc gactcgttct gcgaagggct tcggatgggc gtggaaactt ttcacgctct
      601 caagaagacg cttcacgacc ggaagctctc gaccgcggtg ggcgacgagg ggggcttcgc
      661 gccggatctc aagtcgaact ccgaggcgct cgagctcctg atgaccgcca tcgagagggc
      721 cggctacaag ccgggcgatg acatctcgat cgcgctcgac ctggcctcca gcgagttctt
      781 caaggggggc aagtacatcc tggccggcga caacaacctg acgctcgatt cggccggcat
      841 ggtggaacaa ctcgcgaagc tgtgcgacaa gttccccatc gtatcgatcg aggacggcct
      901 ggccgaggaa gactgggagg gttggaagct cctgaccgac cggctcggca aacgctgcca
      961 gctcgtcggt gacgacctct tcgtgaccaa cacgaagatc ctgaagcgcg gcatcgacac
     1021 cggcgtggcc aattcgattc tcatcaaggt gaaccagatc ggcaccctga ccgagacgct
     1081 ggccgccgtc gacatggcca aaagggccgg ttacagcgcc gtcatgagcc accggagcgg
     1141 cgaaaccgag gacgtgacca tcgccgacct ggccgtggcc accggcgtgg gccagatcaa
     1201 gaccggcagc gccagccgaa gcgaccgcgt ggccaagtac aatcaactcc ttcggatcga
     1261 agaggaactc ggcgacgccg cccgctaccc gggcctgtcg ctcttcagcg gaagaaagta
     1321 gtaccgaggt cgtgtggagg gcgcggggcg cccgtgggag gtggacgacg tgaaccggac
     1381 cagcctgaac gagcaagtga ccaaccgtta ccagcgcatc ttccacgacg gctacgacaa
     1441 cgccgcccag cactggaaga aaggcctggc cctcctcctc gtgacggtca ccggcctctg
     1501 cgcctgggaa ctcgtggccg gcaaccaggg gatcctggcc cgcaagcggg tgaaagccga
     1561 catcgcacgc ctcatcgcag ccaacgcgga cctggcacgc cagcagaagg actacgccgc
     1621 acagaaccgc ggcctggcca ccaacgactt catggcggaa aaggccgccc gcgaaatcct
     1681 ccatcgagcc cggcccggcg aaatcatcta cctcttcgag gaatccgccg ccggcggcac
     1741 ccccatgacg ttttccgacg tcacgatcgt ccaggctccg cccgcgaaag ccgacaagga
     1801 aaagcccgcc acccgttgac atccccaccc gcgggcgtta tggtttcccc cgcttcaatc
     1861 acatcgacaa gttgcggggt ggagcagcct ggtagctcgt tgggctcata acccaaaggt
     1921 cgttggttca aatccaaccc ccgctaccaa atatgacaag gggttacggc ggcgaaaggc
     1981 caccgtgacc ccttttcgct tgcgtggctg ccccagaaga gaatgagggg ttcggcgtcc
     2041 gccgaacccc attccaaaca agatgtgtcg atcccctccc cgtaatcgcg ggaaggccag
     2101 acatccccta ccccaacctc atcttctcca gaaaagcccc ccaccgcgga tcctcgtgca
     2161 gcggccggag cagcgggtcg atcatgacca gcgccacgcc ggtgtcgcgc tgcatgcagg
     2221 cgcgctccag ccactcgaag gcggaatcgg cgtcgccgaa gtcggcgtag atctcggcga
     2281 tctggaacgc ggagtgggag gcactgatgc ggatcagctc ctcgagggcg gcccgggcgt
     2341 cgtcgaggcg gccggtgtgc cgatggtgga gagccaggcc ggtaaggcgg ccccagtccg
     2401 agggttcgtt ggcaagctcg gccatggcct cgtccggccg gcccagcgtg atgaggatgt
     2461 tggagagcac cattcgatcg gacacgacgt tcggttccag ctcgatgcac ttgcggattg
     2521 cgatctcggc ttccgcgtac cggtcagccg cccggaggat cctgccctgc acgctgtagc
     2581 cgatggcggc caggggatcg agctccacgg ctttgcggga aaacaccagc gcctcgtcga
     2641 aacggccggt caccagcaag gccatcgcca ggccattcag tgcgtcggaa ttctccggcg
     2701 ccagttccac tgccttgcgc agggacgctt ccgcgcgtgg ccagtcccac atggaccaga
     2761 gcagcaccgt tccgcgtgcc cgatgggcct gggccagttc cgggtccagc gtgatggcgc
     2821 gctgtgcggc atccagggcg gcggaattgg aatcgaggac gttggcgaac cccttgagcg
     2881 cggagacggt gtgggcccag gagagctggg cccaggcgag cgcgtgcccg ggttccatcg
     2941 cgaccacctg cttcaggatc tcgatggccc tgacgttgtc ttcggcggtc aggcggtcgg
     3001 cgtggtagcg accctgcagg tagagccggt gcgcctcgcc gttctccccg cggccgcgac
     3061 cggccacggc cacttcggcg cgggcctggc cgctggcgtt ggagtcggcg gcttcgccga
     3121 ggagcgtggt ccggagttcc ttgaccacgg cctgggcgat gtcgtcctgc accgcgaaga
     3181 tgtcgtcgag cgtgcgatcg tacgattccg accagaggtg gaagccgtcc gcgaccttga
     3241 cgagttgcac cgagatgcga acgcggttcc ccgtcttccg aacgcttccc tcgacgagcg
     3301 cggcgacgtt
//
LOCUS       DASVOD010000101         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_893861, whole genome shotgun sequence.
ACCESSION   DASVOD010000101 DASVOD010000000
VERSION     DASVOD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893861"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2239
                     /locus_tag="VF720_01980"
     CDS             <1..>2239
                     /locus_tag="VF720_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878146.1"
                     /translation="ISGNASFTLRTIDGTLPVGAGTVTIRFRAQSDNGYSDEDGAYLS
                     TCGLAAIDDIVLSGAIVHSANFESGTNGWVSVTPVSPASQEYADLEDIANLSLPTTFP
                     CNTCGVRDSVLIFVDPSGQHPAGQSNVAISPWIDLKAGGDVGRSELFVTFNAYSGTNT
                     PVSPNLAYGVQYYPWVCPVTGLIGTSPIIYNNAFFFFGNPTCGQVTYSSLRSYAPLAA
                     EQIRVVLGPHGYLDPNTSNGPWFDNIRVGTRGPNTAPPIVLNYRFQDNFAADGSLNPL
                     STSRLDSSLSGPDYSRDTLAVTTTGSNAETRVVFRVTPGQFLPQPVLVSRSARWTPEP
                     TLGPGWYSARLDTAQQGTNPPYVYYNNVVEWMTTFHEADPGFQGTDTSLDPTDPGHLL
                     NDIFPDDLLTPGSRLDYFFAARFRPPDPRNPSGTSWYVTPDTTGGNWFEMEVLPSSMA
                     ADTTWNCVLIVDGHDYESRADHDLEESALTLALGSDGPHARPRFDRWDINSASNMRLG
                     RPAGSAVGATLVQLSAYRRVLWYAGYSSSVRAPGADDVAILEPWLKAAGAGSRGFWLN
                     GATSAGSMNSGTPKTFLNSTLGVTHTCGAIRAAACPSGSLADLSFCLPLTAAPAPAFP
                     PTGSASLAQNGCPDGLNYGLLAVNGAVPTATANLRYLKNGTPTDAASVTVNAPSGFVY
                     RSVVDAFPVSKLRRTPGNPNDPAQCANVTPVYERTLDVLAWLGMPDCPAASVTGVGPV
                     PEGVSR"
BASE COUNT          380 a          791 c          672 g          396 t
ORIGIN      
        1 cgatcagtgg caatgcatca ttcacgctcc gcacgatcga cggcacgctc ccggtgggcg
       61 ccggtacggt taccatccgc ttccgtgcgc agtccgacaa tggctacagc gatgaggacg
      121 gtgcctacct cagcacgtgc ggcctggccg ccatcgatga catcgtgttg tcgggtgcca
      181 ttgtgcacag cgccaacttc gagagcggga cgaacgggtg ggtctccgtg acgccggtca
      241 gcccggcctc gcaggagtat gccgacctcg aagacatcgc caacctctcg cttccgacca
      301 cgtttccctg caacacctgc ggcgtccgcg attccgtgct catcttcgtc gatccgtcgg
      361 ggcaacaccc ggcagggcag agtaacgtgg cgatctcccc gtggatcgac ctcaaggccg
      421 gcggcgatgt gggacgctcc gaactgttcg tcacgttcaa tgcctactcg gggaccaata
      481 cacccgtctc gcccaacctg gcctacggcg tgcagtacta cccgtgggta tgtccggtga
      541 cggggctgat cggtacgagc ccgatcatct ataacaatgc tttcttcttc ttcggcaatc
      601 ccacctgcgg gcaggtgacc tattcctcgc tgcgcagcta tgcacccctg gccgccgaac
      661 agatccgcgt ggtgctcggg ccacacggtt acctcgatcc gaatacgagc aacggtccgt
      721 ggttcgacaa cattcgcgtg ggaacgcgcg ggccgaacac ggccccgccg atcgtcttga
      781 actatcgatt ccaggacaac ttcgcagcgg atggatcact caatcccctc tcgacttcga
      841 ggttggacag ttcgttgagt ggacccgatt acagccgcga cacgctggcg gtcacgacga
      901 ccgggtccaa cgcggagacg cgggtcgttt tccgagtcac gccggggcag ttccttcccc
      961 aaccagtgct cgtgtcacgg agtgcccgct ggacgccgga gcccaccctc ggccccggct
     1021 ggtactcggc gcggctcgat acggcccagc agggcacgaa tccgccctat gtctattaca
     1081 acaatgtcgt cgagtggatg acgacgttcc acgaagcgga tcccggcttc cagggcaccg
     1141 atacgtccct tgatcccacc gaccccggcc atctcctgaa cgacatcttt ccggacgacc
     1201 tcctcacgcc cggaagccgg ctggactatt tcttcgcggc ccgcttccgt ccccccgacc
     1261 cgcgcaatcc gagcggcacg tcgtggtacg taacgccgga cacgaccggg ggcaactggt
     1321 tcgagatgga ggtgcttccg tcatcgatgg cggccgacac cacctggaac tgcgtactga
     1381 tcgttgatgg tcacgactat gaatccaggg cggatcacga tctcgaggag tcagccttga
     1441 ccttggcgct cggatcggac ggtccgcacg cacggccccg cttcgatcgc tgggatatca
     1501 acagtgccag caacatgaga ctgggccggc ctgccggaag cgccgtcggc gccacgctgg
     1561 ttcagctctc cgcctaccgg cgggtgctct ggtatgccgg atactcgagc agcgtacgag
     1621 cgccaggggc cgacgacgtc gccatcctgg agccctggct caaggccgcc ggcgccggat
     1681 cgcggggatt ctggctcaac ggtgccacct ccgccggctc gatgaacagc ggaacgccaa
     1741 agacgttcct caactccacg cttggcgtca cgcatacgtg tggcgcgata cgcgcggccg
     1801 cttgcccgtc cggatcgctg gccgacttga gcttctgcct gccgctgacc gcggcgccgg
     1861 cgcccgcttt ccccccgacc ggcagcgcca gtcttgcgca gaacggctgt cccgatggcc
     1921 tcaactacgg cttgctcgcc gtgaacggcg ccgtcccgac ggcaaccgcc aatctgcgct
     1981 acctcaagaa cggcacaccg accgacgccg cctcggtcac cgtcaacgca ccgagcggct
     2041 tcgtctatcg cagcgtggtc gacgcctttc cggtgtccaa gctccggcgc acacccggca
     2101 accccaacga tccggctcag tgcgcgaacg tgacccccgt gtatgagcgc acgctcgacg
     2161 tgctggcctg gctggggatg ccggattgcc cggccgcctc cgtcacgggc gtgggacccg
     2221 tgccggaagg tgtgtcccg
//
LOCUS       DASVOD010000102         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_7537, whole genome shotgun sequence.
ACCESSION   DASVOD010000102 DASVOD010000000
VERSION     DASVOD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7537"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /gene="rimP"
                     /locus_tag="VF720_01985"
     CDS             complement(<1..282)
                     /gene="rimP"
                     /locus_tag="VF720_01985"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX7878147.1"
                     /translation="MTRLISDVLTGTGYELVEVELAGQASRSIVRVFIDHPKGVGLDD
                     CARVTRLLGDHLDAAGDIGLRSYVLEVSSPGIDRPLKGALDFLRFRGESA"
     gene            complement(426..1364)
                     /locus_tag="VF720_01990"
     CDS             complement(426..1364)
                     /locus_tag="VF720_01990"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7878148.1"
                     /translation="MRRPRGRGLPACIPPAAYFLFLLVGVWVTVALPSDAPIFDGPAA
                     YRLVEKQCAFGPRNPGSPGHRACLAWMEAELRVTGAVVKRQTFSYTPPGSKAPIPMCN
                     LVATFNPAAERRILLCAHWDTRPWGDNDPDSTRRDEPILGANDGASGVAVLLQLARHF
                     QSEPPPYGIDIVLFDGEDMGSKQNPRGWFLGSREYGRRSLGQKPPDHAVLLDMVGDSD
                     LLFYVEGNSLQAVPEQVAWFWAGAAEVAPAFFTDQVGYFVEDDHLPLIAAGIPCLDLI
                     DFNYPHWHTHADTPDKVSVSSLQVVGDVLVHLLYRR"
     gene            complement(1364..>3937)
                     /locus_tag="VF720_01995"
     CDS             complement(1364..>3937)
                     /locus_tag="VF720_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878149.1"
                     /translation="YRSSRNIPKFRANNDIIFDGEDSGLDITENKSQAINLSLRRLSN
                     QYTPWIIRNTIDAMAFRIGTAKSYNRATTSVDSSRAVTAGANWSMRIADPFGIPLPNR
                     WKLSVIPDAVSASYNISDQKSVNYNRDAADPTVLKKTRDNQLKNEGLGFNSSWHLLPP
                     ITFAISSQRDLSNANFERFVIKGVDYRPTLTVAGIDLGREVTRTERLTFTQSFNLTSF
                     FRPRLSWTGDYSENHSPDITTSADIDSTSDTTDSTFVPPDLKTIENRSSTQVSWTIPV
                     SAIFRKLAGTGRTTPRAAPAAGGTYKPPTDSPLAPPPSGGVPPNTQSPHPLAPPPDTT
                     GVIPPGGTSDPEDGNLDAGQAATNTAAPPETPPPPTNDPKEIERRRKADEEARKKEGE
                     RVKKEAEQAKKDAEAAARWEAEEKKRVAKEAEEARKAEEEARKKEAKRLAEEKKKAES
                     HGGIGGKKKKSEPSPPADSTLVPPAGAGSGGNPSLAPPSTAATPAPGDSAAGAPATTG
                     GMTKAQADAEARRRQEETARRAEEERRRKEAEAERARKAAEPPVSLGAFLFRDLIQIG
                     DIRTSFNVTDRNTANRVHGSPDLAYRLGLSSGLEGVTLADGSRVGSSNSVSASGNSAI
                     TLLKDITVDANFQWTETENETDRALSRTANTIWPGLRMNLNSLEKKLKLGRFFTSFSA
                     SSGYERRSEEQGTGLNRRERVTTSSSWRPLLQVDANWRNGVKTQFSADHSSQSQEQFE
                     GSQAGAGVVSTHNTDTYRMTVAKSLVARGGLRGGARSTIDLNLDFTYSKDSIFNDFSN
                     GNVDNGKATDNFQTRFSTSYRFTTNVSGSMQLNYGQRRDYEQGTADRTLGLQATAAIT
                     F"
BASE COUNT          600 a         1271 c         1315 g          751 t
ORIGIN      
        1 ggccgactcg ccacggaagc gcaggaaatc cagcgctccc ttcagcggcc ggtcgatccc
       61 cggcgaggat acttcgagca cgtacgaccg aaggccgatg tccccggcgg catccaggtg
      121 atccccgaga aggcgggtca cccgggcgca gtcatcgagg ccgaccccct tcgggtggtc
      181 gatgaagacg cggacgatcg accggctggc ctgacccgcc agctcgactt cgaccagttc
      241 gtatcccgta ccggtgagaa cgtcggagat cagccgggtc actcgatcgg cccggtcccc
      301 agacatcgct tgaatcgaat cccctaaaac gaaatagagt ggacggtttg gcccactcgc
      361 atcgagaagc tactacaagg attcgcggac tggcaagacc cgtttttccg agcccggcga
      421 cggtatcagc gccggtagag gaggtggacc aggacgtctc ccaccacctg gaggctcgaa
      481 acggacacct tgtccggcgt gtcggcatgg gtgtgccagt ggggataatt gaagtcgatc
      541 aggtcgaggc agggaatccc ggcagcaatc agggggagat ggtcgtcctc cacgaagtag
      601 cccacctggt ccgtgaaaaa ggccggggct acctcggccg cacccgccca gaaccaggcc
      661 acctgctcgg ggaccgcctg gagcgagttc ccctccacat agaataggag gtccgagtcc
      721 ccgaccatgt caagcaggac tgcgtgatcc ggaggcttct gcccgagcga gcgccgcccg
      781 tactcacggg acccgaggaa ccaccccctg gggttctgct tgctccccat gtcctccccg
      841 tcgaagagga cgatgtcgat cccgtacggc ggcggctcgg actggaagtg ccgggccagt
      901 tgaaggagga cggccacgcc cgacgccccg tcgttggctc cgaggatcgg ctcatcccgg
      961 cgggtggagt ccgggtcgtt gtcgccccac ggacgcgtat cccaatgggc acagaggagg
     1021 atgcgccgtt cggccgcggg gttgaatgtg gccacgaggt tgcacatcgg aatcggcgct
     1081 ttcgaccccg gtggcgtgta ggaaaacgtc tgccgtttca cgacggcacc cgtcaccctg
     1141 agttcggctt ccatccaggc aaggcacgcc ctgtgtcccg gggagcccgg gttgcgggga
     1201 ccgaatgcgc attgcttttc cacgagccgg tatgcggcgg ggccgtcgaa gatcggcgcg
     1261 tcgctcggca gggcaacggt cacccacacc cccaccaaca ggaacaggaa gtaggccgcc
     1321 gggggtatgc aggcgggaag tccgcgtccg cgaggccgtc gcatcagaag gtgatcgccg
     1381 cggtggcctg gagtccgagg gtccggtcgg cggtcccctg ctcgtagtcc cggcgctggc
     1441 cgtagttgag ctgcatcgac ccggaaacat tcgtcgtgaa gcggtaactg gtcgagaatc
     1501 gtgtctggaa gttgtcggtg gccttgccgt tgtcgacatt cccgttggag aagtcgttga
     1561 agatcgagtc cttcgaatag gtgaaatcca ggttcaggtc gatggtgctc cgcgcgccgc
     1621 cccgcaatcc gcctcgcgcc accagcgact tggccaccgt catccggtag gtgtccgtgt
     1681 tgtgcgtgct cacgacgccc gcgccagcct ggcttccctc gaactgctcc tggctctgcg
     1741 aggaatggtc cgccgagaac tgcgtcttga cgccattgcg ccagttcgcg tccacctgaa
     1801 ggagaggccg ccagctgctg gatgtggtta ccctctcacg ccggttgagg cccgtcccct
     1861 gctcctcgct gcgccgctcg taaccgctcg acgccgaaaa cgacgtgaag aagcgcccga
     1921 gcttcagctt cttctccagg gaattgaggt tcatgcgcaa ccccggccag atcgtgttgg
     1981 cagtccggct caaggcccga tcggtctcgt tctccgtttc ggtccactgg aagttggcat
     2041 cgacggtgat gtccttgagc aacgtgatgg ccgagtttcc cgaagccgaa acggaattcg
     2101 agctccccac ccgggagccg tcggccagcg tcaccccttc gagaccgctg gagagtccga
     2161 gccggtaggc caggtccggg cttccatgca cgcggttggc cgtgttccga tcggtaacat
     2221 tgaaactggt ccggatgtcg ccgatctgga tcaggtcgcg gaagagaaac gcgccgagtg
     2281 acaccggcgg ttcggccgcc ttccgggcac gctcggcctc ggcctccttc cggcggcgct
     2341 cttcttcggc ccgccgagcg gtttcctcct ggcggcgacg ggcttcggcg tcggcctgtg
     2401 ccttggtcat gccaccggtg gtcgccgggg ccccggcagc cgaatcgccg ggtgccggcg
     2461 tggccgccgt ggacggaggc gcaagggacg gattgccccc ggagccggcc ccggcgggcg
     2521 gaaccagggt cgagtcggcc ggcggtgacg gctccgactt tttcttcttg ccgccgatgc
     2581 cgccatgaga ctcggccttc tttttctcct ccgccaggcg cttggcctcc ttcttcctgg
     2641 cctcctcctc cgccttacgt gcttcctcgg cctccttcgc cacccgcttc ttctcctcgg
     2701 cctcccatct cgcggcggcc tcggcatcct tcttcgcctg ttccgcctcc ttcttcacgc
     2761 gttcgccctc ttttttcctt gcctcctcat cggccttccg gcgccgttcg atttccttgg
     2821 ggtcgttggt tggtggcggc ggcgtttcgg ggggcgccgc cgtgttggtc gcggcctgcc
     2881 ccgcgtccag gttgccatcc tcgggatcac tggttccgcc tggcgggatg acccccgtgg
     2941 tatccggcgg cggcgccagc gggtgcgggc tctgcgtgtt cggaggaacc ccacccgacg
     3001 gcggcggcgc gagaggcgag tcggtaggcg gcttgtaggt accaccggcc gccggagccg
     3061 cccggggagt cgtacgaccc gtgccggcca gcttgcggaa gatcgcggag acgggaatcg
     3121 tccagctcac ctgcgtgctg ctccggttct cgatggtctt gagatcggga ggcacgaagg
     3181 tcgagtcggt cgtatcgctg gtgctgtcga tgtccgcgct cgtggtgatg tccggtgaat
     3241 ggttctcgga gtagtcgccg gtccacgaca ggcgcggccg gaagaacgag gtcaggttga
     3301 acgactgggt gaacgtgagt cgctccgtgc gggtgacttc gcgcccgagg tcgatgccgg
     3361 caaccgtaag cgtcggccgg taatccactc ccttgatcac gaagcgttcg aagttggcgt
     3421 tcgagaggtc gcgctggctg ctgatcgcga acgtgatcgg cggcaggaga tgccagctgc
     3481 tgttgaagcc caggccctcg ttcttcagct ggttgtctcg ggtcttcttc agcaccgtgg
     3541 ggtcggcggc atcgcggttg tagttcaccg acttctggtc ggagatgttg taggaggccg
     3601 agacggcatc cgggatcacc gacaacttcc accggttcgg caaggggatg ccgaaggggt
     3661 cggcgatccg catcgaccag ttcgcgccgg ccgtcaccgc gcggctcgag tcgacggagg
     3721 tcgtggcgcg gttgtagctc ttggccgtcc cgatccggaa agccatcgcg tcgatcgtgt
     3781 tccggatgat ccatggggtg tactggttgc tgaggcggcg caacgacagg ttgatcgcct
     3841 ggctcttgtt ctcggtgatg tcgagccccg aatcctcccc atcgaagatg atgtcgttgt
     3901 tggcgcggaa tttcgggatg ttgcgcgacg accggta
//
LOCUS       DASVOD010000103         4778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_946198, whole genome shotgun sequence.
ACCESSION   DASVOD010000103 DASVOD010000000
VERSION     DASVOD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946198"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1976
                     /locus_tag="VF720_02000"
     CDS             <1..1976
                     /locus_tag="VF720_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878150.1"
                     /translation="RGPQGGEFAPAGALTSPGQQSMGQVDGRWRLGDRATIDGEFALS
                     GQDLNTFSPIDDDDNSGNAGRLNLAVAPVPLAFSGLRLGDIAATAEHRHLSEHFAPLG
                     RIDASFTYDRDWNLPPRTGGLAEDRTGGVVTWRPIVPLSLIGDLAHLSGSGRSSDRRN
                     ARAELSGRVSATGSLLVVDSEVDTGTADGRLTRETLGARSRVKFLVPSARYEAEERTD
                     PVSGVGARYWLGGGDIGVDGLGPVHLTIGAERRVDDRKGAAGSDTLGWQEDTDAVTKR
                     GSLSLSNWNALTASLLYQTRDVERSDGGTSETDLAQFDVSRRSSDAAFGVDFHYQVGT
                     NGVESRTRMLVFVGEGMGSYDEFGNLFPGGGYDLVEGPLGAEEVISDVDASLHLELVP
                     YRARDATGALGWLHKNVGWNATGRVEERSRLPLGRFNHLLDFGAFQDTANSLGGRVTL
                     RQTAEIFPSSRTATIRLSQEYDDIANYQFSNFREDRNESLYGVGVRWTPGPLWTLEGT
                     QKFGTRSQEVTAGDASPRVRRANVLESIGQATWRPSPIVRALLALTWHQESESDDAYA
                     TALDLNPRFSWSIGQKGRLEAGGRWVEADRHGGYTGIGGFSSLALQDRIEATVDGDYR
                     LLDVLTLGAGLAGRRPEGSRFIVDGRMEVRAYF"
     gene            1981..3699
                     /locus_tag="VF720_02005"
     CDS             1981..3699
                     /locus_tag="VF720_02005"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7878151.1"
                     /translation="MLLFLGTLMLAAPAWGQDARVVVDHWKFDGLAAIDDRDAADRLG
                     LVIGGTLDTVEFKGRLGSLLTALGDEGYLDATARPAITPASEGHADFRVVVVEGARAV
                     FGPIVHDGTDSLTAIVRPRPPDAGRPFRRVELERNINEWLDAYDESGFPYARIRPVDV
                     SVDRMARPPVVTVRMRHEPGPRVVLAALDLKGARSTRPEAVARAIGFTPGTVYQQSRI
                     EEGIRRLERTGLVAAVGDVELLPGDDPTHSRLRLTVREASSGSLAGIVGYSGLDERLS
                     GYLDFRLNNIGGTGRRLAARWNAIEDASTLYRLTYREPFLFGKPLDAAVGLEHTIFDT
                     LYTTTRSELSLFWRVGGRTEAHVTAGLDHVVVTSGLNRNQSSGRYAFGVDFDARTPAL
                     APRSGGLLSGRLARGKTLSGLYGDDVTGTLESILSAGLRAESYHPLGRHGVLALLLTG
                     RSIETSATPVPQYELLPLGGALTLRGYREEQFYTPGYVLGQFEYRILADQNGRGVFLF
                     TDVATFAPQEAEATLWPKFDEVKVGYGAGIRMGSRVGRLGLDYGLAAGEGPLDGRIHL
                     RAETEF"
     gene            complement(3931..4545)
                     /locus_tag="VF720_02010"
     CDS             complement(3931..4545)
                     /locus_tag="VF720_02010"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEX7878152.1"
                     /translation="MDELLHRLSAWAATLSPVLLLLVAFAAGVIETIFPPFPSDSLLI
                     ALSFTAARGTTHPVALAVSAALGSFLSLYLLYLAGRSRLEGPVRRLIARLHLDTEARL
                     SGWFARWGYLTILASRFLPGIRGPITFLAGTYGLRPARMAAALLASCLLWNAAVVLVG
                     WVAGRNWDGSAGGLGWIGLAIAVWVSVAWLGGAWALRRGASTRR"
BASE COUNT          775 a         1590 c         1706 g          707 t
ORIGIN      
        1 accgcgggcc gcagggagga gaatttgccc cggccggcgc gctcacctcg cccgggcagc
       61 aatcgatggg tcaggtggat ggccgctggc gcctcggtga ccgcgccacg atcgatggcg
      121 agttcgcgct gtcggggcag gacctcaaca ccttttcgcc gatcgatgac gatgacaaca
      181 gcggcaacgc cgggcgcctg aacctggcgg tggcgccggt gccactcgcc ttcagcggcc
      241 tgcgattggg agacatcgcc gccacggccg agcatcgcca cctctcggag catttcgcgc
      301 ctctcggccg tatcgacgcc tcgttcacct acgaccgcga ttggaacctt ccgccccgca
      361 ccggcggact cgcggaagat cggaccggcg gcgtcgtgac ctggcggccg atcgtgccgc
      421 tctcgttgat cggcgacctg gcgcacctgt ccggaagcgg gcggtcgagc gatcgacgga
      481 atgcccgcgc cgaactctcg gggcgcgtga gcgccaccgg cagcctgctg gtcgtggaca
      541 gtgaagtcga caccggcacg gccgacggcc gcctgacccg cgaaacgctg ggtgcgcgca
      601 gtcgcgtgaa attcctcgtc ccctcggccc gctacgaggc ggaggaacga accgatccgg
      661 tgagcggggt cggtgcgcgc tactggctgg gtggtggcga tatcggggtc gacggcctgg
      721 ggccggttca cctgacgata ggcgccgagc gccgcgtgga cgaccggaag ggggcggcgg
      781 ggagcgacac gctcggttgg caggaggaca ccgatgccgt cacgaagcgg gggtcgctca
      841 gcctttcgaa ctggaacgcg ctcacggcct cgctccttta ccagacgcgt gatgtcgagc
      901 gttcggacgg cggcacgagc gaaaccgacc ttgcgcagtt cgacgtgtca cgccgctcat
      961 cggacgccgc cttcggcgtc gacttccact accaggtggg caccaacggc gtcgaatcgc
     1021 gcacgcgcat gctcgtcttc gtgggggagg ggatggggag ctacgacgag ttcggcaacc
     1081 tcttccccgg gggcggctac gacctggtgg agggtcccct tggcgccgag gaagtgatct
     1141 ccgacgtcga tgcgagcctg catctcgaac tcgtgccgta ccgcgcccgc gacgcgaccg
     1201 gcgcgctggg atggctgcac aagaacgtcg ggtggaatgc cacgggacgg gtggaggagc
     1261 gctcgcgcct gccgctcggc cgcttcaacc atctcctcga cttcggcgcc ttccaggaca
     1321 ccgccaactc gctcggcgga cgcgtgacgc tccggcagac ggccgagatc ttcccctcca
     1381 gccgcaccgc cacgatccgg ctcagccagg aatacgacga catcgccaac taccagttct
     1441 ccaacttccg cgaggaccgg aacgagagcc tgtacggcgt gggcgtgcgc tggacgccgg
     1501 gcccgctctg gacgctcgag ggaacgcaga agttcggcac ccgctcgcag gaagtgacgg
     1561 cgggggacgc gtcgccccgc gtgcggcgcg ccaacgtcct ggaatcgatc gggcaggcca
     1621 cctggcggcc gagcccgatc gtgcgggccc tgcttgcgct cacgtggcac caggagtcgg
     1681 agagtgacga cgcctatgcc acggccctcg acctgaatcc gcgtttctcc tggtcgatcg
     1741 gccagaaggg gcggttggaa gcgggtgggc gctgggtgga ggccgatcgc cacggcggct
     1801 acacgggaat cggcggattt tcctcgctgg cacttcaaga caggatcgaa gccaccgtcg
     1861 acggcgacta ccggctgctc gacgtcctca cgctcggcgc cgggcttgcc gggcggcgcc
     1921 ccgaggggag ccgcttcatc gtggatgggc ggatggaggt gcgggcctat ttctaggatc
     1981 gtgcttctct ttcttggcac gctcatgctt gccgctcccg cgtggggcca ggacgcgcgc
     2041 gtcgtcgtcg accactggaa gttcgacggg ctcgccgcca tcgacgaccg ggatgcggcc
     2101 gaccggctcg gcctcgtcat cggtggcacg ctcgacacgg tggagttcaa gggtcggctc
     2161 ggctccctcc tcaccgcgct gggcgacgaa gggtatctcg acgccacggc gcggccggcc
     2221 atcacgcctg cgtccgaggg gcatgccgat ttccgagtcg ttgtcgtgga gggagcgcgg
     2281 gcggtcttcg gcccgatcgt gcacgacggg acggacagcc tgaccgcgat cgtccggccc
     2341 aggccgcccg acgccggccg ccccttccgg cgtgtcgagc tcgagcggaa catcaacgag
     2401 tggctggatg cctacgacga gtcgggattt ccctacgctc gcatccggcc ggtggatgtc
     2461 tccgtggacc ggatggccag acctccggtc gtgacggtca ggatgcgtca cgaacccggc
     2521 ccgcgggtcg tgctcgcggc gctcgacctg aagggggcgc ggtcgacacg gcccgaggcg
     2581 gtggcacggg ccatcggctt cacgccgggc acggtgtacc agcaatcgcg catcgaagaa
     2641 ggaattcggc ggctggagcg gaccggcctc gtggccgcgg tgggggatgt ggaactcctg
     2701 ccgggcgacg acccgacgca cagccgcctg cgcctcaccg tacgcgaggc gtcgagcggc
     2761 agcctggccg gaatcgtcgg ctactcgggc ctggacgagc ggctgtcggg atacctcgac
     2821 ttccgcctca acaacatcgg cggcaccggc cggcgcctcg ccgcgcggtg gaacgccatc
     2881 gaggacgcca gcacgctcta tcgcctgacg tatcgcgagc cgttcctatt cgggaagccg
     2941 ctcgacgcgg ccgtcggcct cgagcacacc attttcgaca cgctctacac gaccacgcgc
     3001 tccgagctct cgctcttctg gcgcgtggga gggcgcaccg aggcccacgt gaccgccggc
     3061 ctcgaccacg tggtcgtgac gtcgggcctg aatcgcaacc agtcgagcgg acgatacgct
     3121 ttcggcgtcg acttcgacgc ccgcacgccg gcgctggccc cgcgttccgg cggcctcctg
     3181 agcggccgac tcgcacgcgg aaagacactc tccggcctct acggcgacga cgtgaccggc
     3241 acgctggagt cgatcctctc cgccggcctg cgcgccgaga gttatcaccc gctgggacgg
     3301 cacggcgtcc tggcgctcct cttgaccggg cgcagcatcg agaccagcgc cacacctgtc
     3361 ccgcagtacg agttgttgcc gcttggcggc gcgctgacgc tgcgcggcta tcgggaggaa
     3421 cagttctaca cgcccggcta cgtcctcgga caattcgagt accggatcct cgccgaccag
     3481 aacggcagag gcgttttcct cttcaccgac gtggcgacat tcgcccccca ggaagccgag
     3541 gccaccctgt ggccgaagtt cgacgaggtg aaggtggggt acggggccgg catccggatg
     3601 ggctcgcgcg tgggacgact cgggcttgat tacggactcg cggcgggaga ggggccgctg
     3661 gatggacgga ttcatctgcg ggcggagacg gagttctagg ggagcgggtg ggcgcgcggt
     3721 tagtcgtatc gcccgatgtc ttgcattgta agacagtgcg caaatggaag gaccgcgacc
     3781 ctcctggacg ttcgtttcac aaggacgttg gcggggcagg gcctcaacga agggcccaat
     3841 ctgacattga aacaggaggc caactcggtc cgaaccgagt tggccaacat tgtattcagg
     3901 tcaggtctga gttcgaccaa acgcgcccgg ctaccgacgc gtcgatgccc cccgtcggag
     3961 cgcccaggcg ccacccaacc acgccaccga cacccacacg gcaatggcca ggccgatcca
     4021 gccgaggcct cccgcggatc catcccagtt ccgcccggcg acccatccga ccaggacgac
     4081 ggccgcgttc cagaggaggc aggacgccag gagggcggcg gccatccgcg ccggtcgcag
     4141 gccataggta ccggcaagaa aggtgatcgg accccggatg cccgggagga atcggctggc
     4201 caggatggtg agatagcccc agcgggcgaa ccagccggag aggcgggcct cggtgtcgag
     4261 gtggaggcgg gcgatcaggc ggcggacggg gccctcgagt cggctgcggc cggcgaggta
     4321 gagaaggtag aggctgagga acgagccgag ggctgccgac accgccaacg cgaccgggtg
     4381 cgtggtgccg cgcgccgcgg tgaagctcaa ggcgatcagg agactgtcgc tcgggaaggg
     4441 tggaaaaatg gtctcgatga cgccggcggc gaaggccacc aggagcagga gcaccgggga
     4501 aagagtggct gcccacgcgg aaagacggtg gaggagctcg tccatggcgt cccattctgg
     4561 accctccgcc cgccgcctga tagtctcctc gactcgattg gggtgccgac gtttacaagg
     4621 agattgcatg acagcgaagt cgcgtgggtg gcggttgccg cccggagtgg ccgccgcgac
     4681 actggcggtt ctggggatgg ggtacgcggc gtccgtgagg gcggaaacgc tcgacatggc
     4741 cggcgtgaag gtggaagtcg tcgagcggaa gctcgaca
//
LOCUS       DASVOD010000104         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_998839, whole genome shotgun sequence.
ACCESSION   DASVOD010000104 DASVOD010000000
VERSION     DASVOD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998839"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1786)
                     /locus_tag="VF720_02015"
     CDS             complement(<1..1786)
                     /locus_tag="VF720_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878153.1"
                     /translation="MAGRTIAGKDLALLWEGSGVAGSFHWNPADSLGGLGIEAAFDRL
                     DLGRIRRVVEVDLIPTTGIASGRLSLNGIPGLFTFDTSLAGMWGSHPVDTLSVTGRRN
                     GSTIDIERLRLLMPDYDIRQLAGRLDLADGGHLDIGADFAHVQLDSVPLAAVNWLEGR
                     SSGHLDMEMEGFLKSEYPVIGEVTLGLGPTDVFKLPLAGVDGRISFHEGADARLAGVD
                     LRLANGGHVALDGAIHPDGGLDLTLLGETADWSQLEPILAIPGLQGAGRAGGSVKGTG
                     ERPVVDVTGRFSGVQGWGFVADSLDLARLTGPVLPKPELSGLVRTQGLIAVGRKIEAV
                     DLDFAWKEPRLTINRVDLVNADTTASATGWVDFDPAREAMNGTLSGGHLAMGRFDWIP
                     DRDIVFSGVGERFMLAPTRWSSDAGAATLAATFDNAAGRMNLAVDDMDIDLLILSGPD
                     APPEFGGGRLTGRLVLNGPNDNPDPSGAIAIGGLRWRTAVVDSIFADFAANGRRVTVN
                     RSIVRIDSGEVAASGALDLTASAWHVANDYMAKKEVPWQQVDLHDLVVDAHDIDLVTW
                     QAFQPPPEPTHGVIRSHAVLNGTLAAPSI"
BASE COUNT          259 a          630 c          646 g          263 t
ORIGIN      
        1 cgatggaggg ggccgccagc gtgccgttca gcacggcgtg gctccggatg acgccgtgcg
       61 tcggttctgg tggtggctgg aaggcctgcc acgtcacgag gtcgatgtcg tgcgcgtcga
      121 ccacgaggtc gtgcaggtcc acctgctgcc agggaacctc ctttttggcc atgtagtcgt
      181 tggccacgtg ccaggccgac gccgtaaggt cgagcgcccc gcttgccgcc acttccccgg
      241 agtcgatccg gacgatcgag cgattgacgg tgacgcgccg tccgttcgcg gcgaagtcgg
      301 cgaagatcga gtcgacgacc gccgtccgcc agcgcagccc gccgatggcg atggcgccgg
      361 acgggtcggg gttgtcgttc gggccgttca acacgaggcg gccggtgagc cgcccgccgc
      421 cgaactcggg gggcgcgtcc ggcccggaga ggatcagcag gtcgatgtcc atgtcgtcga
      481 cggccaggtt catccgcccg gcggcattgt cgaacgtggc ggccagggtg gcggcgccgg
      541 cgtcggagct ccagcgggtc ggtgccagca tgaagcgctc ccccaccccg ctgaagacga
      601 tgtcgcgatc ggggatccag tcgaagcgcc ccatcgccag gtgcccaccg gagagggtgc
      661 cgttcatcgc ctcgcgggcc gggtcgaaat cgacccagcc ggtggcggac gccgtcgtgt
      721 cggcgttgac caggtccacc cggttgatgg tgaggcgcgg ttccttccag gcgaagtcga
      781 ggtcgaccgc ctcgatcttc ctgccgacgg cgatcagccc ctgcgtgcgc acgagccccg
      841 acagctccgg tttgggaagt accggcccgg tgagccgggc gaggtcgagc gagtcggcca
      901 cgaagcccca cccctgcacg ccgctgaagc gtcccgtcac gtcgacgacg gggcgttccc
      961 ccgtcccctt gacgctgccg ccggcgcgac cggcgccttg cagccccggg atggccagga
     1021 tcggctcgag ctggctccag tcggctgtct cgccgaggag cgtgagatcg aggccgccgt
     1081 ccgggtggat cgccccgtcg agcgccacgt gcccgccgtt cgcaaggcgc aagtcaacgc
     1141 cggcgaggcg cgcgtcggca ccctcgtgga aggagatgcg cccgtcgacg ccggccagcg
     1201 gcagcttgaa gacatccgtc gggccgaggc ccagcgtgac ctcgccaatc acgggatatt
     1261 cggacttcag gaacccctcc atctccatgt cgagatggcc ggacgagcgc ccctcaagcc
     1321 agttcaccgc ggccagcggc accgagtcga gctgcacgtg cgcgaagtcg gcgccgatgt
     1381 cgaggtggcc cccgtcggcc aggtcgaggc gaccggccag ctgccggatg tcgtagtcgg
     1441 gcatgaggag ccgcagccgt tcgatgtcga tggttgagcc gttccgccgt cccgtcacgc
     1501 tgagcgtgtc gaccgggtgc gatccccaca tgccggcgag gctcgtgtcg aacgtgaaga
     1561 gccccgggat cccgttgagc gagaggcggc cgctggcgat ccccgtggtg ggaatgaggt
     1621 ccacttccac cacgcggcgg atccgcccga gatccaggcg gtcgaaggcg gcctcgatgc
     1681 cgagcccccc gagcgagtcg gcggggttcc agtggaacga cccggccacg ccgctcccct
     1741 cccataggag cgccaggtcc ttcccggcga tggtgcgccc ggccacggtc acgcgccc
//
LOCUS       DASVOD010000105        11133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_946217, whole genome shotgun sequence.
ACCESSION   DASVOD010000105 DASVOD010000000
VERSION     DASVOD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11133
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946217"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3538)
                     /gene="rpoC"
                     /locus_tag="VF720_02020"
     CDS             complement(<1..3538)
                     /gene="rpoC"
                     /locus_tag="VF720_02020"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX7878154.1"
                     /translation="MITKSEGKEVRRKTNFTAIKIQLASPEVIKGWSSGEVTKPETIN
                     YRSFKPEKDGLFCERIFGPVKDWECNCGKYKRIRYRGVICDRCGVEVTQAKVRRERMG
                     HIDLAVPVSHIWYFKGVPSRIGHLLNMSVRDLERVLYYESYVMIDPGNTHHKIKDLLT
                     EDQYAELANDDTQQFNAKMGAEAIRELLGNMELDSLAAELRTVVKIETSAQRKKETLK
                     RLRIVEAFRQSGNKPEWMILDVVPVLPPDLRPLVPLEGGRFATSDLNDLYRRVINRNN
                     RLKKLKEIKAPEVILRNEKRMLQEAVDALFDNGRRSRAVRGQGNRPLKSLSDMLKGKQ
                     GRFRQNLLGKRVDYSGRSVIVVGPELKLHQCGLPKSMALELFKPFIIKNLEDKGFVST
                     VKSAKRLVERERPEVWDILGEIIKGHPVMLNRAPTLHRLGIQAFEPVLVEGKAIRIHP
                     LVCAAFNADFDGDQMAVHVPLSWEAQIEAHELILSTNNILSPASGRPLATPSQDIVLG
                     CNYLTKLRPLAEGKRPRSFPDATSVRGAWDHGAVALHEPVRVRLEGKIVETTVGRILF
                     NDILPAGIGFSNEEMKKKTLESLVARCFRLYGNKKTAIFLDDLKRTGFEYATMAGITV
                     GIDDIMIPKEKDAIIRKHQAEVDDIARKYKDGAITDGERYNKVIDTWTRATTAVEAVT
                     FTGLAKDRNGFNPIFMMADSGSRGSKEQIRQLAGMRGLMARPQKKITGGFGEIIESPV
                     IRNFKEGLTVLEYFISTHGARKGLADTALKTADAGYLTRRLVDVAQDVIINEPDCQSI
                     LGLDMGPLKEGEEIIEPLGERVLGRVAAEDVFDAIANEIIVRAGEEIREEHADRIEES
                     SIDHVKIRSVLTCAARRGVCALCYGRNLATGRMVDLGEAVGVIGAQSIGEPGTQLTLR
                     TFHIGGVAGRIVEQSSIKARANGTVKYPKLPVVEIGEGVHSGKWIVTGHKGDLELVDD
                     EGRTRHRYSPPYGATIMVADGAAVEEGQPLFEWDTYNTPILTERNGVVRFGDIKERVT
                     VRDVLDENTGLRQQVIVDDKEKVLQPHIDVIGAAGQRLAHYPLPTGAMLLIKDGEKVT
                     AGTVVAKIRRDISKTRDITGGLPRVSELFEARKPKDAATVTEIDGIVRFAGITRGMRK
                     IVVENENSENREYLVPQGKHLVVQEGLHVRAGE"
     gene            complement(3603..7463)
                     /gene="rpoB"
                     /locus_tag="VF720_02025"
     CDS             complement(3603..7463)
                     /gene="rpoB"
                     /locus_tag="VF720_02025"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009183388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX7878155.1"
                     /translation="MKNNGSRRSYSKIEEKWDVEIPNLLDVQLQSFRGFLQDDVPPEQ
                     RKNEGLQEVFTSVFPISDTREMFSLEFVSFAIGDPKYSVEECQERDLTFSAPLKCTLR
                     LRIREVEDGQKRDKEIIEQEVYLGELPLITSKGTFLINGAERVIVSQLHRSPGVFFDE
                     AIHPNGKKLFSARIIPYRGSWVEFSLDVNDIMYVHIDRKRKLPVTILLKALGYTNAKD
                     ILDLFYATEAIELGSGRSQKDLSLEGRVVAEDVVDTETGEILIETNDELTVDRIKDLA
                     KAGIKEIKLFRIALQDEADVIRNTLKKDSSTGEDDALSRIYNLLRPGEPPRSETAKDI
                     LNRLFFNPKRYDLAKVGRYKLNQKLNHEKLLGGPAKMKKLGLARPSDEHTILSPEDFV
                     AIVKYLVMLRTAEEDNGVVAETDDIDHLGNRRVRSVGELLANQFNIGLTRMARIIRER
                     MSLQDSEQVTPYDLINARTISAVVQSFFGSSQLSQFMDQTNPLAELTHKRRLSALGPG
                     GLTRDRAGFEVRDVHYTHYGRMCPIETPEGPNIGLISSLSTYARVNELGFLESPYRKI
                     SGGTVDKKHWSWLSADIEDTCNIAQANEPVAESGDFEKASLVVRNRGEFPAVTAKEVD
                     FMDVSPIQLVSPAAALIPFLEHDDANRALMGSNMQRQAVPLLVTQAPLVGTGLEGKVA
                     ADSGALIHARRDGVVESVAGDSIAIKYDRDKTEANVVDFSEASGLDLYTLTKFKRSNQ
                     DTCINQRPLVKEGDRVVKGDVIADGAATKDGELALGLNVRVAFMPWGGYNFEDAILVS
                     ERLIKNDAFTSIHIEEFELQVRDTNRGVEEITREIPNVGDDAVKNLDDEGIIRIGAPV
                     KAGDILVGKVTPKGETDLSPEERLLRAIFGEKAGDVRDASLKAPPGMDGIVIDIKVFS
                     RREKDEATKKQDKKKIDRLKRYARKERTHIIEIRDQEVVNLLVDQLANRIVSAKTGEE
                     LIKAGKKITKQSLARMELDELPWNSPLTKSRKINDTVWKQMELAAGALDRIEKNLEKE
                     VDKITRGDELPPGVVKLVKVYVSKKRKLSVGDKMAGRHGNKGVVAKILPEEDMPYMED
                     GSAVDMVLNPLGVPSRMNIGQIMETHLGWAAHKIGIKVATPVFAGATVEEIKSTLREA
                     ELADDGKSVLFDGRNGRRFDERVTVGYIYMLKLSHLVDDKIHARSIGPYSLVTQQPLG
                     GKAQFGGQRFGEMEVWALEAYGAAFTLQEMLTVKSDDVSGRSRIYEAIVKGENPPDPG
                     VPESFNVLVKELQSLCLDVRFEEG"
     gene            complement(7594..7998)
                     /gene="rplL"
                     /locus_tag="VF720_02030"
     CDS             complement(7594..7998)
                     /gene="rplL"
                     /locus_tag="VF720_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX7878156.1"
                     /translation="MSETTYAPEVADIVGKIKNMSILQVNDLVKAIEEEFGVSAAMPM
                     MAGPMMMGAGGGGAAAAAEEKTEFDVELASAGDKKIQVIKVVRELTGLGLKEAKDLVD
                     GTPKMVKEKVSKADADKMKAALEEQGAVVNIK"
     gene            complement(8139..8657)
                     /gene="rplJ"
                     /locus_tag="VF720_02035"
     CDS             complement(8139..8657)
                     /gene="rplJ"
                     /locus_tag="VF720_02035"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX7878157.1"
                     /translation="MRPEKVELVNTLEAKLKDAKGVCLADFTGMTVERMSDLRVRCRK
                     AGVEFKVIKNTLTRRALSEDLRAPMDPYLVGPTAVALSLTDEVAPAKVLADFAKEFQS
                     PKIKAGLVAGRVYNEAAVKELASLPGKDVLLGKLLGGLKQPVQKLHSALSSPLRNLAT
                     VLKAHAEKLPAA"
     gene            complement(8676..9389)
                     /gene="rplA"
                     /locus_tag="VF720_02040"
     CDS             complement(8676..9389)
                     /gene="rplA"
                     /locus_tag="VF720_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003333393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX7878158.1"
                     /translation="MVKHGKRFRRLADQHAGDPNHKHADIAEAIKVVKSCASAKFDET
                     VDVAVRLGIDPRHSDQQVRGTVVLPHGTGKTIRVLVLAKGEKAKEAEAAGADLAGADE
                     YLAKIKEGWLDFDAVVATPDMMAEVGKLGKVLGPRGLMPNPKTGTVTFDVTKAVKELK
                     AGKIEFRVDKGGNLHVPVGKASFGPDALYDNVRAFLNEILRAKPAAAKGAYVKSVTLS
                     TTMGPGIKIDPVAVSGPKA"
     gene            complement(9402..9830)
                     /gene="rplK"
                     /locus_tag="VF720_02045"
     CDS             complement(9402..9830)
                     /gene="rplK"
                     /locus_tag="VF720_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX7878159.1"
                     /translation="MAKKVLDKTIKLQIPAGAANPAPPIGPALGQAGVNIMEFCKAFN
                     AATQGQPGLIIPVVISVFKDKSFTFITKTPPAAVLLKRAAGLAKGSGEPNRNKVGKVT
                     SAQVKEIAELKKPDLNAASLEAAMSMVAGTARSMGIDVTD"
     gene            complement(9964..10506)
                     /gene="nusG"
                     /locus_tag="VF720_02050"
     CDS             complement(9964..10506)
                     /gene="nusG"
                     /locus_tag="VF720_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEX7878160.1"
                     /translation="MTSSVDKRWYVIHTYSGHENKVKTNLERTIRNNGVEEKFGQILV
                     ATEDHAEMKDGKKVISRRKTFPSYVLVEMLLDDETRTLVANVPGVTRFIGSGADPVPL
                     TLPEVQRILGQMDTTKPKEPLEVPYKVGEHVKVTDGPFTDFTGVVDEVNAERGKLKVM
                     VSIFGRATPVELDFLQVKPI"
     gene            complement(10512..10703)
                     /gene="secE"
                     /locus_tag="VF720_02055"
     CDS             complement(10512..10703)
                     /gene="secE"
                     /locus_tag="VF720_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEX7878161.1"
                     /translation="MTLVEQTRTFFRETAVEVRKVAWPTRKELTESTMLVIVTVTILM
                     FLVFFIDRGFSAIVELILG"
     gene            complement(10750..10826)
                     /locus_tag="VF720_02060"
     tRNA            complement(10750..10826)
                     /locus_tag="VF720_02060"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10790..10792),aa:Trp,seq:cca)
     gene            complement(10846..10995)
                     /gene="rpmG"
                     /locus_tag="VF720_02065"
     CDS             complement(10846..10995)
                     /gene="rpmG"
                     /locus_tag="VF720_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013709217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX7878162.1"
                     /translation="MRDQITLACGECKRRNYTTTKNKRKHPDRVEYKKYCPFCRVHKP
                     HKETR"
     gene            complement(11011..>11133)
                     /gene="tuf"
                     /locus_tag="VF720_02070"
     CDS             complement(11011..>11133)
                     /gene="tuf"
                     /locus_tag="VF720_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819280.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7878163.1"
                     /translation="DNVKMSIELITPIAMDEGLRFAIREGGRTVGAGVVSKILE"
BASE COUNT         1667 a         3535 c         3664 g         2267 t
ORIGIN      
        1 gttcgccggc gcggacgtgc agaccttcct ggacgaccag gtgcttgccc tgcggaacga
       61 ggtactcgcg gttctcgctg ttctcgtttt ccaccacgat cttgcgcatg ccgcgggtga
      121 tgccggcgaa gcgcacgatg ccgtcgattt cggtgacggt ggcggcgtcc ttcggcttcc
      181 gggcctcgaa gagctcggaa acgcggggca gaccgcccgt gatgtcgcgg gtcttggaga
      241 tgtcgcggcg gatcttggcc acgaccgtac cggccgtcac cttctcgccg tccttgatca
      301 gcagcatggc gccggtcggc agcgggtagt gcgcgagcct ctggcccgcc gcgccgatga
      361 cgtcgatgtg cggctggagc accttctcct tgtcgtccac gatgacctgc tgcctgaggc
      421 ccgtgttctc gtcgaggacg tcgcggacgg tgacgcgctc cttgatgtcg ccgaagcgca
      481 ccacgccgtt ccgttcggta aggatcgggg tgttgtaggt gtcccactcg aacaggggct
      541 gcccctcctc caccgccgcg ccgtcggcca ccatgatggt ggcgccgtaa ggcgggctgt
      601 agcggtggcg ggtgcgccct tcatcgtcga ccagctcgag gtctcccttg tggccggtga
      661 cgatccactt gcccgagtgc actccctcgc cgatttccac gacggggagc ttcgggtact
      721 tgacggtgcc gttggcccgc gccttgatcg agctctgctc gacgatgcgg ccggccacgc
      781 caccgatgtg gaaggtacga agggtgagct gcgtgcccgg ctcgccgatg gactgggcgc
      841 cgatgacgcc gaccgcctcg cccaggtcga ccatgcggcc ggtggcgagg ttacgtccgt
      901 agcagagcgc gcacacgccg cgccgcgcgg cgcaggtgag caccgaacgg atcttgacgt
      961 gatcgatgga cgattcctcg atccggtcgg cgtgctcctc gcggatttcc tcgccggccc
     1021 ggacgatgat ctcgttggcg atcgcgtcga acacgtcttc cgcggccacg cgacccagga
     1081 cgcgctcgcc gaggggttcg atgatttcct ccccctcctt gagcggaccc atgtcgaggc
     1141 cgaggatcga ctggcaatcg ggctcgttga tgatgacgtc ctgcgccacg tcgacgagtc
     1201 gacgggtgag gtagccggcg tcggccgtct tcagggccgt gtcggccaga cccttgcggg
     1261 cgccgtgggt ggagatgaag tactcgagaa cggtgaggcc ttccttgaag ttccggatga
     1321 ccgggctctc gatgatttcg ccgaaaccac cggtgatctt cttctgcgga cgggccatga
     1381 gcccgcgcat gccggccagc tgccggatct gctccttgga gcctcgcgat ccggagtcgg
     1441 ccatcatgaa gatcgggttg aaaccgttgc ggtccttggc gaggcccgtg aaggtgaccg
     1501 cctcgaccgc cgtggtggcg cgcgtccagg tatcgatgac cttgttgtag cgctcgccgt
     1561 cggtgatggc gccgtccttg tacttccggg cgatgtcatc cacttcggcc tggtgcttcc
     1621 tgatgatggc gtccttctcc ttcgggatca tgatgtcgtc gatcccgacg gtgatgccgg
     1681 ccatcgtggc gtactcgaaa ccggtccgct tcaggtcgtc gaggaagatg gccgtcttct
     1741 tgttgccgta caggcggaag cagcgggcca ccagcgactc gagcgtcttc ttcttcattt
     1801 cctcgttcga gaacccgatc cccgcgggga ggatgtcgtt gaagaggatg cggccgacgg
     1861 tcgtctcgac gatcttccct tcgagacgca cgcggaccgg ctcgtggaga gccaccgcgc
     1921 cgtggtccca ggcaccgcgg acgctggtgg cgtccgggaa cgaccgggga cgcttccctt
     1981 cggccagcgg gcggagcttg gtcaggtagt tgcagccgag aacgatgtcc tggctgggcg
     2041 tggccagcgg ccggccggaa gccggggaca ggatgttgtt cgtcgacagg atgagttcat
     2101 gcgcctcgat ctgggcttcc cacgagagcg gcacgtggac ggccatctgg tccccgtcga
     2161 agtcggcgtt gaacgccgcg cagacgagcg ggtggatacg gatggccttc ccttccacca
     2221 gcaccggctc gaaagcctgg atgccgaggc ggtgcagcgt cggggcgcgg ttcaacatca
     2281 cgggatgacc cttgatgatc tcgcccagga tgtcccacac ctccgggcgc tcgcgctcga
     2341 cgagcctttt tgcgctcttg acggtggaga cgaacccctt gtcctcgagg ttcttgatga
     2401 tgaacggctt gaagagctca agggccatcg acttgggcag accgcactgg tggagcttga
     2461 gctccggtcc gaccacgatg accgaacggc cactgtagtc gacgcgcttg ccgagcaggt
     2521 tctgccggaa gcgcccctgc ttccccttga gcatgtcgga aaggctcttg aggggacgat
     2581 taccctggcc gcggaccgcg cgggagcgcc ggccgttgtc gaacagggcg tcgaccgcct
     2641 cctggagcat gcgcttctcg ttgcggagga tgacctccgg cgccttgatc tccttgagct
     2701 tcttcaggcg gttgttgcgg ttgatgacgc gacggtacag gtcgttcagg tcggaggtgg
     2761 cgaaacggcc gccttccagc ggcacgagcg gccgcaggtc gggcggcagg accggcacca
     2821 cgtcgaggat catccactcg ggcttgttgc ccgactggcg gaaggcctcg acgatgcgga
     2881 ggcgcttgag ggtttccttc ttgcgctggg cgctggtctc gatcttgacc accgtgcgga
     2941 gttcggcggc cagggagtcg agctccatgt tgccgaggag ctcgcggatg gcctcggccc
     3001 ccatcttggc gttgaactgc tgcgtgtcgt cgttggcgag ctcggcgtac tggtcctcgg
     3061 tgaggaggtc cttgatcttg tggtgcgtgt tgccgggatc gatcatcacg tacgactcgt
     3121 agtagagcac gcgctccagg tcgcggaccg acatgttgag caggtggccg atgcgagacg
     3181 gcacgccctt gaagtaccag atgtggctga ccggcacggc caggtcgatg tgacccatcc
     3241 gctcgcgccg caccttggcc tgcgtgacct ccacgccgca gcggtcacag atgacgccgc
     3301 ggtagcggat gcgcttgtac ttgccgcaat tgcactccca gtccttgacc ggaccgaaga
     3361 tgcgctcgca gaacaggccg tccttctcgg gcttgaacga ccggtagttg atggtttccg
     3421 gcttggtcac ctctcccgac gaccatccct tgatgacctc gggggatgcc agctggatct
     3481 tgatggcggt gaagttcgtc ttgcgacgga cttccttgcc ttcactcttg gtgatcatga
     3541 gcttcccggt cgaaagaccc gggaagagcg gggactggag tcctgggctc tcctgcatct
     3601 tctcagccct cctcgaagcg gacgtccaga cagaggctct ggagttcctt gaccaacacg
     3661 ttgaacgact ccggcacgcc gggatccggc gggttctcac ccttgacgat cgcctcgtag
     3721 atgcgcgacc gcccgctgac gtcgtcggac ttcacggtga gcatttcctg gagcgtgaac
     3781 gccgccccgt acgcctccag cgcccagact tccatttcgc cgaaacgctg gccgccgaac
     3841 tgggccttgc cgcccagtgg ctgctgcgtg acgagtgaat agggcccgat cgaccgggcg
     3901 tggatcttgt cgtcgaccag gtgggaaagc ttgagcatgt agatgtagcc gacggtgacg
     3961 cgctcgtcga aacggcggcc gttgcggccg tcgaagagga ccgacttgcc gtcgtcggcc
     4021 agctcggcct cgcggagcgt ggacttgatc tcctcgacgg tggcgccggc gaacaccgga
     4081 gtggccacct tgatgccgat cttgtgggcc gcccacccga ggtgcgtttc catgatctgc
     4141 ccgatgttca ttcgggacgg cacgccgagg ggattcagca ccatgtcgac ggccgagccg
     4201 tcctccatgt agggcatgtc ctcctcgggg aggatcttgg ccacgacacc cttgttgccg
     4261 tgacggccgg ccatcttgtc gccgaccgag agcttgcgct tcttggacac gtacaccttc
     4321 accagcttga ccacccccgg cggcagttcg tcgccgcggg taatcttgtc cacttccttc
     4381 tcgagattct tctcgatccg gtcgagggcg ccggccgcca gctccatctg cttccagacg
     4441 gtgtcgttga tcttgcggct cttggtgaga gggctgttcc acgggagctc gtcgagctcc
     4501 atgcgggcca gtgactgctt ggtgatcttc ttgccggcct tgatcagctc ctcgccggtc
     4561 ttggccgaga cgatgcggtt ggccagctgg tcgacgagga ggttcaccac ctcctggtcg
     4621 cggatctcga tgatgtgcgt gcgctccttg cgggcgtagc gcttgagtcg gtcgatcttc
     4681 ttcttgtcct gcttcttggt ggcttcgtcc ttctcgcggc gcgagaagac cttgatgtcg
     4741 atgacgatgc cgtccatgcc cggcggggcc ttcagggagg cgtcgcggac gtcgccggcc
     4801 ttctcgccga agatggcccg aaggagccgc tcttccggag agaggtccgt ttcgcccttc
     4861 ggcgtgacct ttccgaccag gatgtcgccg gccttgaccg gcgcgccgat gcggatgatt
     4921 ccctcgtcgt cgaggttctt caccgcgtcg tcgcccacgt tcgggatttc acgggtgatt
     4981 tcctccacgc cgcggttcgt atcgcgcacc tggagctcga actcctcgat gtggatcgag
     5041 gtgaaggcgt cgttcttgat cagccgctcg gacacgagga tggcgtcctc gaagttgtaa
     5101 ccgccccacg gcatgaaggc cacccggacg ttcagtccga gcgccagctc gccgtccttg
     5161 gtggcggcgc cgtcggcgat cacgtcaccc ttcaccaccc ggtctccctc cttcacgagg
     5221 ggacgctggt tgatgcacgt gtcctggttg ctgcgcttga acttggtcag cgtgtagagg
     5281 tcgagcccgg acgcctcgga gaaatcgacg acgttggcct cggtcttgtc gcggtcgtac
     5341 ttgatggcga tcgagtcgcc ggccaccgac tccacgacgc cgtcgcggcg ggcgtggatc
     5401 agggcgccgg aatcggccgc caccttcccc tcgagcccgg tgccgaccag cggcgcctgg
     5461 gtgacgagga gcggcaccgc ctggcgctgc atgttggagc ccatcagcgc ccggttggcg
     5521 tcgtcgtgct cgaggaacgg gatgagcgcc gcggccggcg aaaccagctg gatcggcgac
     5581 acgtccatga agtcgacttc cttggccgtg accgccggga actcgccccg gttgcggacg
     5641 accagcgatg ccttctcgaa atctcccgac tccgccaccg gctcgttggc ctgggcgatg
     5701 ttgcaggtgt cctcgatgtc ggcgctgagc caggaccagt gcttcttgtc cacggtgccg
     5761 cccgagatct tgcggtacgg gctctccagg aagccgagct cgttgacgcg ggcgtacgtg
     5821 gaaagcgacg agatcaggcc gatgttcgga ccttcgggcg tctcgatcgg gcacatgcgc
     5881 ccgtagtgcg tgtaatggac gtcgcgcacc tcgaagccgg cgcgatcacg cgtgaggcct
     5941 cccggtccca gggcggacag gcggcgcttg tgcgtgagct cggccagcgg gttcgtctgg
     6001 tccatgaact gggagagctg gctcgagccg aagaacgact ggaccaccgc cgagatggtg
     6061 cgggcgttga tcaggtcgta cggagtgacc tgttcggaat cctggagcga catgcgctcg
     6121 cggatgatcc gggccatgcg cgtcaggcca atgttgaact ggttggccag gagctcgccc
     6181 accgagcgca cccggcggtt gcccaggtga tcgatgtcgt cggtttccgc caccaccccg
     6241 ttgtcttcct cggcggtgcg gagcatgacc aggtacttga cgatcgccac gaagtcctcg
     6301 ggcgacagga tggtgtgctc gtccgacgga cgggcgagcc ccagcttctt catcttggcc
     6361 gggccgccca ggagcttctc gtggttcagc ttctggttca gcttgtagcg ccccaccttg
     6421 gccaggtcgt accgcttcgg gttgaagaag aggcggttca ggatgtcctt ggcggtttcc
     6481 gagcgcggcg gctcgcccgg gcgcagcagg ttgtagatgc gcgagagggc gtcgtcctcg
     6541 ccggtggagg agtccttctt gagcgtgttc cggatgacgt cggcctcgtc ctgcagcgcg
     6601 atccggaaga gcttgatttc cttgatcccg gccttggcca ggtccttgat cctgtcgacg
     6661 gtcagctcgt cgttggtctc gattaggatc tcgccggtct cggtgtccac cacgtcctcg
     6721 gcgacgacgc gcccctcgag cgagaggtcc ttctggctcc gcccggagcc gagctcgatc
     6781 gcctcggtgg cgtagaagag atcgaggatg tccttggcgt tcgtgtaacc gagcgccttg
     6841 agcaggatcg tgaccgggag cttgcgcttg cggtcgatgt ggacgtacat gatgtcgttg
     6901 acgtcgagcg agaactccac ccacgacccc cggtacggga tgatccgcgc cgagaagagc
     6961 ttcttgccgt tgggatggat ggcctcgtcg aagaagacgc cgggcgagcg gtgcagctgg
     7021 ctgacgatga cgcgctcggc gccgttgatc aggaaggtgc ccttggaggt gatgagcggg
     7081 agctcaccga ggtacacctc ctgctcgatg atctccttgt cgcgcttctg gccgtcctcg
     7141 acctcgcgga tgcggagccg gagggtgcac ttgagcggtg cggagaaggt cagatcccgt
     7201 tcctggcact cctcgacgga gtacttggga tcgccgatcg cgaacgacac gaattcgagc
     7261 gagaacattt cgcgcgtgtc ggagatgggg aaaacgctgg taaagacctc ctggagacct
     7321 tcgttcttgc gctgttccgg cggaacgtca tcctggagga agccccggaa ggactggagc
     7381 tggacgtcga ggaggttggg aatttcaacg tcccacttct cctcgatctt gctgtaactg
     7441 cgacgactcc cgttattctt caaagcgcca ccttctttgt tattgggccg tgttatcggg
     7501 gccggaaccg gtggttgcgc accggttccg gctcacgacc ggacgcccct aagggcggta
     7561 ttacctggca actgcgatcc gggccggggc gtcttacttg atgttgacga cggctccctg
     7621 ctcctcgagc gcggccttca tcttgtcggc gtcggccttg gacaccttct ccttcaccat
     7681 cttgggcgtg ccgtcgacca ggtccttggc ttccttgagg ccgaggccgg tcagctcgcg
     7741 cacgaccttg atgacctgga tcttcttgtc gccggccgag gccagctcga cgtcgaactc
     7801 ggtcttctct tcggcggccg ccgcggcacc gccgccaccg gcacccatca tcatcgggcc
     7861 ggccatcatg ggcatggcgg ccgaaacgcc gaactcctcc tcgatggcct tcacgaggtc
     7921 attcacctgg aggatggaca tgttcttgat cttgccgacg atgtcggcca cttcgggggc
     7981 gtaggtcgtc tcggacatgg aacgaaacct ccgtgcgttg gatccggtgt cggattaaga
     8041 gccccgcacc cgcgggattc ggacaaccgg ctgttgaaaa aaaacgtggc gacccggcca
     8101 cccggaattt ccggtgacgg gtcggaaagg ggcgatgtct atgcggccgg aagcttttcg
     8161 gcgtgcgcct tgagcacggt ggccaggttg cgcaacgggc tggagagcgc cgagtggagc
     8221 ttctgcacgg gctgcttgag cccgcccagc agcttgccga ggagcacgtc cttgccgggg
     8281 agcgaggcca gttccttgac ggccgcctcg ttgtacacac ggccggccac caggcccgcc
     8341 ttgatcttcg gcgactggaa ctccttggcg aaatcggcca gcaccttggc cggggccacc
     8401 tcatccgtca gggaaagcgc caccgcggtg ggccccacga gatacgggtc catcggggcg
     8461 cgcaggtcct cggacagggc gcggcgggtg agcgtgttct tgatgacctt gaattccacg
     8521 ccggccttgc ggcagcggac acggaggtcg gacatacgtt ccaccgtcat gccggtgaag
     8581 tccgccaggc agacgccctt ggcgtccttc agcttggcct ccagcgtatt gacgagttcg
     8641 actttttcgg gacgcatcgt ttatctcctc gccgatcagg ccttcgggcc ggaaacggcc
     8701 accggatcga tcttgatgcc gggacccatg gtggtggaca gcgtcaccga cttgacgtac
     8761 gcgcccttgg cggccgccgg cttcgcgcgg aggatttcat tcaggaaggc ccgcacgttg
     8821 tcgtacaggg catccgggcc gaacgaggcc ttgccgaccg ggacgtgcag gttgcccccc
     8881 ttgtcgacgc ggaactcgat cttgccggcc ttcagctcct tgaccgcctt ggtcacgtcg
     8941 aacgtgacgg tgccggtctt ggggttcggc atgaggccgc gggggcccag caccttaccg
     9001 agcttgccga cctcggccat catgtccggg gtggccacga cggcgtcgaa gtcgagccag
     9061 ccctccttga tcttggccag gtattcgtcg gccccggcca ggtcggcacc ggccgcctcg
     9121 gcttccttgg ccttctcgcc cttggcgagg acgagcaccc ggatggtctt gcccgtgccg
     9181 tgcggcagga cgaccgtgcc gcgcacttgt tgatccgagt ggcgcggatc gatgccgagg
     9241 cggacggcca catcgacggt ttcatcgaac ttggccgatg cgcacgactt gacgaccttg
     9301 atggcctcgg cgatatcggc gtgcttgtgg ttggggtcgc cggcgtgctg atcggccagg
     9361 cgacggaaac gctttccgtg cttcacgatt cctcctggtc cttaatcagt gacgtcgatg
     9421 cccatgctgc gggcggttcc cgcaaccatg ctcatggccg cctcgagcga ggcggcgttg
     9481 agatcgggct tcttcagttc ggcgatttcc ttcacctggg cggaagtgac cttgccgacc
     9541 ttgttccggt tcggctcccc cgaccccttg gccagcccgg ccgcccgctt gagcaggacc
     9601 gccgccggcg gggtcttggt gatgaacgtg aacgacttgt ccttgaagac cgagatgacg
     9661 accggaatga tcaggcccgg ctgtccctgc gtggccgcat tgaaggcctt gcagaactcc
     9721 atgatgttga cgcccgcctg tccgagcgcc ggcccgatcg gaggcgccgg attggccgct
     9781 cccgccggga tctgcagctt gatggtcttg tcgagtactt tcttggccat ctctccctct
     9841 cactcaccaa acctgtgagg ccgggaggcc ccaccgaggt tcaacgagac gcctgtcggg
     9901 gacacaaggg actccggtgc ggcgtccgtc cccggggcgc cgtccggtaa tcgagaacga
     9961 tcgtcagatc ggttttactt gtagaaagtc gagctccacc ggggtggccc ggccgaagat
    10021 cgacaccatg accttgagct tgccgcgctc ggcgttgact tcgtcaacca cgccggtgaa
    10081 gtcggtgaac ggcccgtcgg tgaccttgac gtgctcgccg accttgtacg gcacttcgag
    10141 cggttccttg ggcttggtgg tgtccatctg ccccaggatc cgctgcactt cgggcagcgt
    10201 cagcggcacc gggtccgcgc ccgagccgat gaagcgcgtg acgccgggga cgttggccac
    10261 cagcgtccgc gtttcgtcgt cgaggagcat ctccacgagc acgtacgacg ggaacgtctt
    10321 gcgccggctg atgaccttct tgccgtcctt catctcggcg tgatcttcgg tggccaccag
    10381 gatctgaccg aacttttcct ccaccccgtt attgcggatg gtgcgttcca ggttggtctt
    10441 caccttgttt tcatggcccg agtacgtatg gatcacgtac cagcgcttgt cgacgctgct
    10501 cgtcacgtcc gtcatccgag aatcagctcc acgatggccg agaaaccccg gtcgatgaag
    10561 aaaaccagga acatcaggat cgtgacggtg acgatgacca gcatcgtcga ttcggtcagt
    10621 tccttgcgcg tcggccaggc caccttgcgg acctcgaccg ccgtctcccg gaagaatgtc
    10681 cgggtctgtt ccaccagcgt catgtttccc cgtttccggg ccggcagggc cccggagtga
    10741 gtctggtaat ggcaggccag gagggactcg atcccccaac attcggtttt ggagaccgac
    10801 gctctaccaa ttggagctac tggcctagcg atgttcgggc caggatcagc gcgtttcctt
    10861 gtgcggcttg tgcacgcggc agaacgggca gtacttcttg tattccaccc ggtccggatg
    10921 cttccgcttg ttcttggtgg tcgtgtagtt ccggcgcttg cattcgccac aggccagtgt
    10981 gatctggtcg cgcatgttcg ttcccttccc ttattccagg atcttgctga cgacgccggc
    11041 gcccacggtg cgcccaccct cgcggatggc gaaccggaga ccttcgtcca tcgcgatcgg
    11101 cgtgatcagt tcgatcgaca tcttcacgtt gtc
//
LOCUS       DASVOD010000106         3716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_580838, whole genome shotgun sequence.
ACCESSION   DASVOD010000106 DASVOD010000000
VERSION     DASVOD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580838"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VF720_02075"
     CDS             complement(<1..421)
                     /locus_tag="VF720_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878164.1"
                     /translation="MPDGGSVSPEPTVSDATREARIEWLFGEVLEQAEEDREAWLRNA
                     GNDDPGVLAEVRSLLEAYRLAPGLLDEVRPAAVGVPLPSGGLEEGDTLGPWRIIRQLA
                     SGGMGTVYLGERIDDSFRMQVAIKVLSARVVHPGLLSQ"
     gene            complement(414..977)
                     /locus_tag="VF720_02080"
     CDS             complement(414..977)
                     /locus_tag="VF720_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7878165.1"
                     /translation="MSDEWATETLIRATNGSREDAERLFQHVYGELREMAAREMRRES
                     PDHTLQPTELVHEVFFRLVVRDRIRADDRREFLGMACRAMRQVLVDHARRRSRIKRGG
                     GQRPVPIDGLPIPASRDDDTTILALNEALERLMQRHPEKARLVDVHYFAGFTLHESAE
                     ILGLSARTAARHWAFAQAWLVRELSRA"
     gene            complement(1041..1706)
                     /locus_tag="VF720_02085"
     CDS             complement(1041..1706)
                     /locus_tag="VF720_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7878166.1"
                     /translation="MRFTLRSAFVSAVAPLLLMALPGVSGTAWAKGFTLFPFAGYTFP
                     AELDGAGDILPGNIEVKGGLTAGVALGTVTPQNIGIEVMYTYQDNGITFKPVGAPEMD
                     IMDMAIHQIHGNFLFYHPSARHSYTKPYLLIGLGANIFDPGGPNADSETRFTWALGLG
                     FVRQVNEKVGLRVQGRYTPTYLNSSDEGYWCDPYYGCYTVFDSNYLDQWDVTGGLVFT
                     LGN"
     gene            1822..2229
                     /locus_tag="VF720_02090"
     CDS             1822..2229
                     /locus_tag="VF720_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HEX7878167.1"
                     /translation="MNAGTPETRSANDLAVERTDLATQRTVMAADRNLMAWIRTALSM
                     ISFGFTIYKLLQGFHAAGEALPRENTPRNIGLFLTALGTVSMVLGCVEYLGVMKEMRR
                     HQHVRYLRPAFGIALVMSVCGLTMFFVIFLKVL"
     gene            complement(2267..3331)
                     /locus_tag="VF720_02095"
     CDS             complement(2267..3331)
                     /locus_tag="VF720_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX7878168.1"
                     /translation="MGKDSRLARSTRWRRTGLLLLVLLLADAMPVLAAPKTDTVRLNT
                     GDILTGEIKSMERGQLVYSTDTMETVSIKWSRVLRVSSPSWYLVTVEGGAQYYGRLQL
                     ADTDGRLRVAILNRYDDLEMADVVRIEPIESRVWDRISVALSFGLSYTKASEVSQLTT
                     DGNMKYKDRRHLIELGGNLIWTDKGDESARSQQFDTALQYQHTITGRLFGAGIISGEG
                     NDGLGLELRVLGGLGMGYRLLEGGASVLVLVGGLSANREWSTGNETPTNNAEGLINSS
                     YSLYFYESPKTDLNVRGTYYQSLDSDRRRADFSTSARRELIPDFFIVLNYYESYDSQP
                     PSAEGDKTDRGVVLQVSWSK"
     gene            complement(3339..>3716)
                     /locus_tag="VF720_02100"
     CDS             complement(3339..>3716)
                     /locus_tag="VF720_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878169.1"
                     /translation="GLRAAWGKSTSFGYLLYGDLGAQEPYDPMDDTNFYWQAGGAVSW
                     DMREKWRPDIGFTLASSYRSRTSRNEDLGSGGLNSSVTVAYTGSPNFNVGLQTIYQRL
                     KQLEVDNRVGVLGFNFFLRYDFS"
BASE COUNT          634 a         1248 c         1196 g          638 t
ORIGIN      
        1 actgggagag gagcccggga tgcaccaccc gggccgagag caccttgatg gccacctgca
       61 tccggaacga atcgtcgatc cgctctccga ggtacacggt gcccatgcca ccgctcgcaa
      121 gttgccgaat gatccgccag gggccgaggg tatcgccctc ctcgagccca cccgacggca
      181 gggggactcc gacggcggcc ggccgcacct catcgagcag cccgggcgcc agccggtagg
      241 cctcgagaag cgaacggacc tcggcgagca ccccgggatc gtcgttgccc gcattgcgca
      301 gccaggcctc ccgatcctct tccgcctgct ccagcacttc cccgaagagc cactcgatcc
      361 gggcttcgcg ggtggcatcc gaaacggtcg gctccggcga aacgctcccc ccgtcaggca
      421 cgggagagtt cgcgcacgag ccaggcctgg gcaaaggccc agtggcgcgc cgccgtcctc
      481 gcggaaaggc cgaggatctc ggccgattca tggagcgtga atccggcaaa gtagtgcacg
      541 tccaccagcc gggctttctc gggatggcgc tgcatcaggc gctcgagcgc ttcgttcaac
      601 gccaggatcg tcgtgtcgtc gtcccgggag gcgggaatcg gcaggccatc gatcggcacg
      661 gggcgctgcc ctcccccgcg cttgatccgg ctccgtcggc gggcgtgatc caccagcacc
      721 tgccgcatgg cccggcaggc catgcccagg aactcccggc ggtcgtccgc acgaatcctg
      781 tcccgaacca ccagccggaa gaagacctca tggacgagct cggtgggctg cagggtgtga
      841 tcgggactct cgcggcgcat ctcgcgcgcc gccatctcgc gcagctcccc gtaaacgtgc
      901 tggaacagcc gttccgcgtc ttcacgcgag ccgttggtgg cgcggatcag tgtttcagtg
      961 gcccattcgt ccgacacgac aatccccggt ttcgggcgtc acggcgggcg gtagcggcca
     1021 accgccggaa cgggatgcgc tcagtttccg agcgtgaaga ccagcccccc ggtgacgtcc
     1081 cactggtcga gatagttgga gtcgaacacc gtatagcagc cgtagtacgg gtcgcaccag
     1141 taaccttcgt cactggaatt caggtaggtc ggcgtgtagc gcccctgcac gcgaaggccc
     1201 accttctcgt tgacctggcg cacgaagccg aggccgagcg cccaggtgaa acgcgtttcg
     1261 gaatcggcat tcggaccacc gggatcgaag atgttggcgc cgagcccgat gaggagataa
     1321 ggcttggtgt acgaatgacg ggcgctgggg tgatagaaga ggaaattgcc gtggatctgg
     1381 tggatggcca tgtccatgat gtccatctcg ggcgccccga ccggcttgaa cgtgattccg
     1441 ttgtcctggt aggtgtacat gacctcgatg ccgatgttct gcggcgtcac cgtaccgagc
     1501 gccacgccgg ccgtgaggcc ccccttcacc tcgatattgc cggggaggat gtcgcccgcg
     1561 ccgtcgagtt cggcgggaaa cgtgtatccc gcgaagggaa agagggtgaa ccccttggcc
     1621 caggccgtgc cggaaactcc cggcaatgcc atgaggagga gcggagcgac ggccgagacg
     1681 aacgcggaac gaagcgtgaa cctcatgatc atttcctccc cacgaacaga tgccgcggca
     1741 tcgcaggcca atcagcgatc cgcatgatat agaatccgcc cggcaagtca atttgcgggc
     1801 cccgaactcg tgggagatcg attgaacgca ggcactccgg agaccaggtc ggccaacgac
     1861 ctggcggtgg aacggaccga cctggccacg cagcgcacgg tcatggccgc cgaccgcaac
     1921 ctcatggcgt ggatccgcac cgcgctgtcg atgatcagct tcggcttcac gatctacaag
     1981 ctgctccagg gattccacgc cgccggcgag gcgctcccgc gcgaaaatac gccgcgcaac
     2041 atcgggctct tcctcaccgc gctcggcacc gtctcgatgg tcctgggttg cgtcgagtac
     2101 ctgggggtca tgaaggagat gcgccggcac cagcacgtcc gctacctccg gccggccttc
     2161 ggcatcgcgc tcgtgatgtc ggtctgcggg ctgaccatgt tcttcgtgat cttcctcaag
     2221 gttctctgag gcgcctgacc ccctcgccgc cgggtcgggg ggcccgctac ttcgaccagc
     2281 tcacctggag caccacgccc cggtcggtct tgtccccttc cgccgagggg ggctggctgt
     2341 cgtagctctc gtagtaattg agcacgatga agaaatcggg gatcagttcc cgccgcgccg
     2401 aggtgctgaa atccgcgcgc cgacggtcgc tgtcaaggct ctggtagtaa gtcccgcgga
     2461 cattgaggtc ggtcttcggc gattcgtaga aatagagcga gtacgaggaa ttgatcagcc
     2521 cttccgcgtt attggtcggc gtttcgttcc cggtcgacca ctcgcggttg gcgctcaggc
     2581 cgccgaccag aaccagcacg ctcgcccctc cttccagcag ccggtagccc attccgaggc
     2641 ctcccagcac gcggagttcg aggcccagcc cgtcgttccc ctcgccggag atgattcccg
     2701 cgccgaacag tcgcccggtg atcgtgtgct ggtactggag ggcggtgtcg aactgctggc
     2761 tgcgcgccga ctcatccccc ttgtcggtcc agatcaggtt gccgcccagc tcgatgaggt
     2821 gccgccggtc cttgtatttc atgttcccgt cggtggtgag ctgcgagacc tcgctcgcct
     2881 tggtgtagct gagcccgaag ctcaacgcca cactgatgcg atcccacacc ctgctctcga
     2941 tcggctcgat gcgcaccacg tccgccatct caaggtcgtc gtagcggttg aggatggcca
     3001 cgcggagccg gccgtcggta tcggcgagct gcagtcggcc gtaatactgc gcgcctcctt
     3061 ccaccgtcac gaggtaccag ctcgggctgg aaacgcgcag cacccggctc cacttgatcg
     3121 acaccgtctc catggtgtcg gtgctgtaga cgagctggcc gcgctccatc gacttgatct
     3181 cgcccgtcag gatgtcgccg gtgttgagcc ggacggtatc cgtcttgggg gccgccagta
     3241 cgggcatggc atcggccagg aggagtacca gcagcagaag ccccgtccga cgccaccggg
     3301 tggaccgggc caggcgggag tccttcccca cggttctcct acgagaagtc gtaacgcagg
     3361 aagaaattga agccgaggac accgacgcgg ttgtccacct cgagctgctt gaggcgctgg
     3421 tagatggtct ggagcccgac gttgaagttg gggctgccgg tataggccac ggtcacggac
     3481 gaattcaggc cgccgcttcc caggtcctcg ttccgcgagg tccgcgaacg gtaggacgag
     3541 gccagcgtga aaccgatgtc ggggcgccac ttctcccgca tgtcccagct cacggcgccg
     3601 ccggcctgcc agtagaagtt ggtatcgtcc atcgggtcgt acggctcctg cgcgcccagg
     3661 tccccgtaca gcaggtagcc gaacgaggtg ctcttccccc aggccgcccg caggcc
//
LOCUS       DASVOD010000107         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_59363, whole genome shotgun sequence.
ACCESSION   DASVOD010000107 DASVOD010000000
VERSION     DASVOD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59363"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="VF720_02105"
     CDS             complement(<1..711)
                     /locus_tag="VF720_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX7878170.1"
                     /translation="MFNIKRKTIEWGGKTLTLETGRMARQADGAVLATYGETMVLATA
                     VYAKSAKPGQDFFPLTVNYQEKFYAAGKIPGSFPRREGAPSQKETLTSRLIDRPIRPL
                     FVKGFKNEVQVVTTVLAHDLENDPDIVALVAASAALVISGAPFMGPIGGARVGFVDGE
                     YVLNPTTDQMKESRMDLVVAGTDDAVMMVESEIQEMSEDEVLKGVMFAHAGMQPVIQA
                     IIELADHAAKEPFDFQPDD"
     gene            complement(942..1211)
                     /gene="rpsO"
                     /locus_tag="VF720_02110"
     CDS             complement(942..1211)
                     /gene="rpsO"
                     /locus_tag="VF720_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX7878171.1"
                     /translation="MSITAERKAEVIKSHARGDADTGSAEVQVAIMSERISNLTDHFK
                     THKKDNHSRRGLLKLVSARRSLLDHLKKSDVARYQAIIEKLGLRR"
     gene            complement(1226..>2152)
                     /gene="truB"
                     /locus_tag="VF720_02115"
     CDS             complement(1226..>2152)
                     /gene="truB"
                     /locus_tag="VF720_02115"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX7878172.1"
                     /translation="ARRKKGQAISGWINLDKPYDLTSTHAVGRIRRLFDAQKAGHAGT
                     LDPLATGILPIALGEATKTVPFLVDADKAYRFTIAWGRTTATYDREGETIAESDVRPS
                     AAEVEAVLPRFVGEIDQVPPAYSAIKVDGERAYDLARAGETVELASRKVNVFDARVAE
                     VPDADHITLEIECGKGTYVRAIVRDIAEALGACGHVSALRRTRVGQFTEQSAVALELL
                     EDLSYEARLSEALLPVETALDDIPVLAVTDEDAFRLKQGRSVVLVPRQVEAVKARLKP
                     GSRTVSAMAGGSMVALCEMRAGRLEPSRVFNL"
BASE COUNT          336 a          696 c          716 g          404 t
ORIGIN      
        1 gtcgtccggc tggaagtcga agggttcctt ggcggcatgg tccgccagct cgatgatcgc
       61 ctggatgacg ggctgcatgc cggcgtgggc gaacatcacc cccttcagca cttcgtcctc
      121 gctcatctcc tggatctcgg attcgaccat catgaccgcg tcgtcggtgc cggcgaccac
      181 caggtccatc cggctttcct tcatctgatc ggtcgtgggg ttgagcacgt actcaccgtc
      241 cacgaagccg acgcgggcgc cgccgatcgg gcccatgaac ggcgcgccgg agatcaccag
      301 ggcggccgag gcggccacca gggcgacgat gtcgggatcg ttctcgaggt cgtgggccag
      361 caccgtggtg acgacctgaa cctcgttctt gaagcccttc acgaacagcg ggcggatcgg
      421 acggtcgatc aggcggctgg tgagggtttc cttctgggag ggcgcgccct cgcggcgggg
      481 gaagctgccg gggatcttgc ccgcggcgta gaacttctcc tgatagttca cggtcagcgg
      541 gaagaagtcc tgaccgggct tcgcgctctt cgcgtaaacc gcggtggcca gcaccatcgt
      601 ctcgccatag gtggcaagca ccgcgccgtc ggcctgacgg gccatgcggc cggtttcgag
      661 cgtgagcgtc ttgccgcccc actcgatggt cttgcgcttg atattgaaca tttctgcttc
      721 tttctcatcc cgcgggcgta ttcccggcgg gggcggacag gggatcggtc ttccgaaatc
      781 ctctccaatg gcacaggcgg tgaggatctc ttgtcgaccc tcggcgtttg tggagctcac
      841 ttgccccggt cgccgcatcc atcccccggc ccgtgacgga ggagcggcct caaataagaa
      901 acggacccgg ttcccgggcg ggaaacgagt ccgctgaaga actagcggcg caggccgagc
      961 ttctcgatga tcgcctgata acgagccacg tcggacttct tcaggtggtc gaggagcgaa
     1021 cggcgggcgg agaccagctt cagcaggccc cggcgcgagt ggttgtcctt cttgtgcgtc
     1081 ttgaagtggt cggtgaggtt cgagatccgt tcggacatga tcgccacttg gacttcagcg
     1141 ctgcccgtat ccgcgtcgcc gcgggcatgg gacttgatga cttcggcttt gcgttcggcc
     1201 gtgatcgaca tcgggtttct ccgctctaga gattgaagac acgcgagggt tcgagccgtc
     1261 ccgcacgcat ctcgcagagc gccaccatcg atccgcccgc catggctgaa acggttcttg
     1321 agcccggctt gagcctggct ttgaccgctt ccacctgtcg gggaacgaga acgaccgatc
     1381 gtccctgctt taatcggaag gcgtcttcgt cggtcacggc cagcaccggg atgtcgtcca
     1441 gtgcggtctc gaccggaagc aatgcctccg acagtcgggc ctcatagctt aaatcctcaa
     1501 gcaattccag tgcgaccgca gattgctcgg tgaactggcc cacccgcgtg cggcgaagcg
     1561 cgctgacgtg gccgcacgct cccaacgcct cggcgatgtc gcggacgatc gcgcggacat
     1621 aggtgccctt gccgcactcg atctccaacg tgatgtggtc ggcgtccggg acctcagcca
     1681 cccgagcgtc gaagacgttc accttgcgcg acgccagctc gacggtctcg ccggcccggg
     1741 ccaagtcgta ggcgcgctcg ccatccacct tgatggccga ataggctggc gggacctggt
     1801 cgatctcgcc cacgaagcgc ggcagcacgg cctcgacctc cgcggcgctc gggcgcacgt
     1861 cggactcggc gatggtctcg ccctcgcggt cataggtcgc agtcgtgcgg ccccaggcga
     1921 tggtgaaacg ataggccttg tcggcgtcca ccaggaacgg gacggtcttg gtggcctcgc
     1981 ccagggcgat gggcaggatg ccggtggcca gcgggtccag ggtgccggcg tgccccgcct
     2041 tctgggcgtc gaacagccgc cggatgcgac cgaccgcatg ggtcgaggtg aggtcgtagg
     2101 gcttgtcgag attgatccag cccgagatcg cctggccctt cttgcggcgg gc
//
LOCUS       DASVOD010000108         3883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_59419, whole genome shotgun sequence.
ACCESSION   DASVOD010000108 DASVOD010000000
VERSION     DASVOD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59419"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..743)
                     /gene="pdxT"
                     /locus_tag="VF720_02120"
     CDS             complement(81..743)
                     /gene="pdxT"
                     /locus_tag="VF720_02120"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEX7878173.1"
                     /translation="MTTVEKTRTPRVGVLAVQGDFAAHLAALERLGVPAVEVRTPAEL
                     SALDGVILPGGESGAHIRILKENGMWDALRAFHSSGGALWGTCAGLILLAKNVTNPPQ
                     DSLGLIDIDVVRNGWGRQIDSFEADVPFTDWGDIAAPAASEPAVLRLVFIRAPRISRV
                     GPEARVLLSIEGEPIAVVQERVLVTTFHPEMTGDASIHRWFVERVVVAGGDSVSHSRS
                     AT"
     gene            complement(763..1644)
                     /gene="pdxS"
                     /locus_tag="VF720_02125"
     CDS             complement(763..1644)
                     /gene="pdxS"
                     /locus_tag="VF720_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018466229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEX7878174.1"
                     /translation="MKEYTLRLKTGLAEMLKGGVIMDVTNADQARIAEEAGAVSVMAL
                     ERVPSDIRKDGGVARMSNVKVIKEIMAAVSIPVMAKVRIGHFVEAQVLESLGVDYIDE
                     SEVLTPADDRYHVDKFAFKTPFVCGCRNLGEALRRIGEGAAMIRTKGEAGSGNIVEAV
                     RHMREVQSHIRKLHGMRTDELMAEAKELGAPYEIVRWVAEHGKLPVPNFAAGGVATPA
                     DAALMMQLGAEAVFVGSGIFKSGDPAVRARAIVLATTHYKDAAKIAEVSEELGDAMPG
                     LDVSKLPEGELMQTRGW"
     gene            complement(1655..3190)
                     /locus_tag="VF720_02130"
     CDS             complement(1655..3190)
                     /locus_tag="VF720_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX7878175.1"
                     /translation="MRILIDRDSRNPLYEQVKGALQRLIVDGVLNPGDELPASRILAR
                     DLSLNRGTITAAYDELVADGLLYRHVGRGTFVANRDQLASVLGTSKPANRAAAGSIRW
                     PELYANDPLKEQDPLSPQLARWAQRDGMISFAGGVPDSSLFPTDEFRLALNRALKEEG
                     PRLLMYGTTAGHRPFIEFLRHYLVERGVGATPDEILIVNGSQQGIDLIARTLVAPGDS
                     ILVEDPTYYGALNLFRTLRARIIPVPVDDHGLNVDALERILTHERPKFLYTMPTFQNP
                     TGVSMSLERRRRLVRLCADRNVPVLEDDFDGDLLYDGDNIPPLKGLPEGRDVLYTGTF
                     SKMLFPGIRLGWVVAPAPVIERLAAAKQVADLSTSLLFQAAMVRFAQGKRLQRHAEDV
                     CREYRKRRDALLVVLQKEMPEGVHWTKPAGGFSLVVKLPAPMDAAEVLSHAADLGVIY
                     QPGRVFSLSGEKGLLRLSFGNSKIDQINEGVRRLAKAVRDEINRNHKASSRRLAVAGA
                     PPV"
     gene            complement(3352..>3883)
                     /locus_tag="VF720_02135"
     CDS             complement(3352..>3883)
                     /locus_tag="VF720_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX7878176.1"
                     /translation="LHHVGDRRNDPARALSPDVQGLGKGHGLNTRSLERLVNLLDSAF
                     RVPGTRWRFGLDGIIGLIPGLGDMVTAAVSLLVLIVAHRERVPATIIGRMLLNIFFDF
                     AIGEVPLVGDVADFFYKSNRKNLELLKRHAHGVEPPRAIDRAVVLGAIILLLAMTGLS
                     VWVSWQVLQWLIHHPL"
BASE COUNT          681 a         1253 c         1276 g          673 t
ORIGIN      
        1 agtacgcatg acttttcggt tctcgatgca tgtgacccgg ccttgactca actggccgtg
       61 cggctgactg gctgaaattt tcatgtcgcc gaacgtgaat gcgacacaga gtccccaccc
      121 gccaccacta cccgctccac aaaccaccga tgaatcgagg cgtcccccgt catctccgga
      181 tgaaacgtgg tcacgagcac gcgctcctgg acgacagcga tgggctcgcc ctcgatcgac
      241 aggagcacgc gagcctcggg cccgacccgc gaaattcgcg gggccctgat aaataccagc
      301 ctcaggacag ccggttcgga tgccgctggc gcggcgatgt cgccccaatc ggtgaagggg
      361 acgtccgctt caaatgaatc gatctggcgg ccccagccgt tgcggactac atcgatatca
      421 atgagcccca gtgagtcctg cggcgggttc gtgacatttt tcgccaacag gatcaacccc
      481 gcgcacgttc cccacagggc gccaccggat gaatgaaacg cgcgaagcgc gtcccacatc
      541 ccgttttcct tgaggatgcg gatatgcgcc ccgctctcac ctccgggcag gatgacaccg
      601 tccagtgcgg acagctccgc cggcgtgcgc acttcgacgg caggtactcc cagccgttcc
      661 agcgccgcca ggtgcgccgc aaagtcaccc tgaacagcga ggacgccgac acgcggcgtc
      721 ctcgtctttt caacagtcgt cattggtcgg cgatccgcga cgctaccagc cccgcgtctg
      781 catcaattcg ccttccggca gcttcgacac gtcgaggccc ggcatggcgt cgcccagttc
      841 ctcggacacc tcggcgatct tggcggcatc cttgtagtgc gtcgtggcca gcacgatcgc
      901 acgggcgcgg acggccggat caccggactt gaagatgcca ctgccgacga acacagcctc
      961 ggcgccgagc tgcatcatga gtgctgcgtc ggccggcgtg gccacgccgc cggccgcgaa
     1021 gttgggcacc ggcagcttgc cgtgctcggc cacccagcgg acgatctcgt agggcgcgcc
     1081 cagttccttg gcctcggcca tgagttcatc ggtgcgcatg ccgtgtagct tgcggatgtg
     1141 gctctgcact tcgcgcatgt ggcggacggc ttcgacgatg ttgccggagc cggcttcgcc
     1201 cttggtgcgg atcatggcgg cgccttcgcc gatgcggcgg agggcttcgc ccaggttgcg
     1261 gcagccgcag acgaacgggg ttttgaaggc gaacttgtcg acgtgatagc ggtcgtcggc
     1321 cggcgtgagc acttcgctct cgtcgatgta atcgacgccg agcgactcga gcacctgcgc
     1381 ttccacgaaa tgaccgatgc ggaccttggc catgaccgga atggacacgg cggccatgat
     1441 ttccttgatg accttcacgt tggacatgcg ggccacgccg ccatccttgc ggatgtcgga
     1501 cggcacgcgc tcgagcgcca tcacgctgac ggcgccggct tcttcggcga tgcgggcctg
     1561 gtcggcgttg gtcacgtcca tgatgacgcc gcccttgagc atctcggcca gcccggtctt
     1621 gagacgaagc gtgtattcct tcatgtcatc ctcctcaaac gggtggcgcg ccggccacgg
     1681 cgaggcgtcg ggaagacgcc ttatggttgc ggttgatctc gtcccgcacg gcctttgcga
     1741 gccggcggac gccttcgttg atctggtcga ttttcgagtt cccgaacgag agccgcagca
     1801 gtcccttctc acccgagagc gagaagacgc gacccggctg gtagatgacg ccgagatccg
     1861 cggcgtgaga gagcacttcg gccgcgtcca tcggtgcggg gagcttgacc accagcgaaa
     1921 aaccgcccgc cggcttcgtc cagtgcaccc cctcgggcat ctccttctgg agcaccacga
     1981 ggagcgcgtc gcgacgcttg cgatattccc tgcatacgtc ctcggcgtgg cgctgcagcc
     2041 gctttccctg cgcgaagcgg accatggctg cctggaagag gagcgaggtg gagaggtcgg
     2101 cgacctgctt ggctgcggcc aggcgctcga tgaccggcgc cggcgccacc acccagccga
     2161 ggcggatgcc ggggaagagc atcttcgaga aggtgccggt gtagaggacg tcgcgcccct
     2221 cgggaagacc cttgagcggc gggatgttgt cgccgtcgta gaggaggtcg ccgtcgaagt
     2281 cgtcttccag caccggcacg ttccggtcgg cgcagaggcg caccagccgg cggcgccgct
     2341 cgaggctcat cgacacgccc gtcgggttct ggaacgtggg catcgtgtag aggaacttgg
     2401 gacgctcgtg cgtgaggatg cgctcgagtg catcgacgtt gaggccgtgg tcatccaccg
     2461 gcaccggaat gatccgggcc ctgagcgtgc ggaacaggtt caacgcgccg tagtaggtcg
     2521 ggtcttccac caggatcgaa tcgcccggcg ccaccagcgt gcgcgcgatc aggtcaattc
     2581 cctgctggct gccgttcacg atcaggattt cgtccggggt ggcgcccaca ccccgctcca
     2641 ccaggtagtg gcggaggaat tcgatgaacg ggcgatggcc ggcggtggtg ccgtacatga
     2701 ggagtcgcgg cccctcttcc ttgagggcgc gattgagcgc caggcggaac tcgtcggtcg
     2761 gaaagagcga ggagtcgggc acaccgccgg cgaacgagat catcccgtcg cgctgggccc
     2821 agcgggcgag ctgcggcgaa agcgggtcct gctccttgag cggatcgttg gcgtagagct
     2881 cgggccagcg gatggagccg gccgccgcgc ggttggccgg cttcgacgtg ccgaggacgc
     2941 tggccagctg gtcgcggttg gccacgaagg tgccgcgccc gacgtggcga tagagaaggc
     3001 cgtcagccac cagctcgtcg taggcggcgg taatcgtgcc gcgattcagg gagaggtccc
     3061 gggccaggat gcggctggcc ggcagttcat cgcccgggtt caacacgcca tccacgatca
     3121 ggcgctgcag ggcgcccttc acctgctcgt acagggggtt tcgggagtcg cgatcgatca
     3181 ggattctcat ctggggagca cctccggact ggatacgacc gagaggaaat ggtactcccc
     3241 caatggcccc gaggaaggtc caatcatcac attttacggt ccaatcaacg aattattgag
     3301 ccaatttgtt cctaatttgt cgaatattgt ccaaaattgg cacgaattgg cctagagcgg
     3361 atggtggatg agccactgga gcacctgcca gctgacccag acgctcaggc cggtcatcgc
     3421 aagaagaaga atgatggccc ccaggaccac cgcccggtcg atggcgcgcg gcggttcgac
     3481 gccgtgggcg tgccgcttca gcagctccag gttcttccgg ttcgacttgt agaagaagtc
     3541 ggccacgtcc ccgacgaggg gcacctcgcc gatcgcgaag tcgaagaaga tgttgaggag
     3601 catccggccg atgatcgtgg ccggcacccg ttcccggtgg gccacgatca ggacaagcag
     3661 tgacaccgcc gcggtcacca tgtccccgag gccggggatg agcccgatga ttccgtcgag
     3721 cccgaaccgc cagcgcgtcc cgggaacccg gaacgcgctg tcgagcaggt tcaccaatcg
     3781 ttcgagcgag cgggtattca ggccgtgccc tttgccgagc ccttgaacat caggcgatag
     3841 agcacgagca ggatcattgc gccgatcacc gacatgatga agc
//
LOCUS       DASVOD010000109         1913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_424815, whole genome shotgun sequence.
ACCESSION   DASVOD010000109 DASVOD010000000
VERSION     DASVOD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424815"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF720_02140"
     CDS             complement(<1..277)
                     /locus_tag="VF720_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878177.1"
                     /translation="MALLNTIARRLFRDLGLKLAAIFLATALYLHVHRETERTIDLRV
                     PLAWRCASADPGRGALPDSVTVRVRATAAQIDRLVAGPLMLQVDLCGA"
     gene            complement(288..719)
                     /locus_tag="VF720_02145"
     CDS             complement(288..719)
                     /locus_tag="VF720_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878178.1"
                     /translation="MSVHRHPFRLTLPATLLVIGPALVTLVAAPGCSEDPPTGPHIDV
                     VIKIRDTTYIPSNVTIEVNQRVEWQNWSRDTRTVTSGVSPEDPAAGEILDVSLAGYPS
                     GEAIGGRLQRQFTEPDTFYYFSREVPDGYTGDFLGVIIVTP"
     gene            858..>1913
                     /locus_tag="VF720_02150"
     CDS             858..>1913
                     /locus_tag="VF720_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7878179.1"
                     /translation="MPEFRGPAAVLRPVRALVALFVLLMPVIFPAPASARVVVLGFDG
                     ADARLVERWMNEGKLPNLDRLRREGTWSPLGTTTPPQTPVSWTSFATGINPGRHGIFD
                     FLSRDVATYQPGYAMFDIRDVKVGAGKANGPLAGGLAGLLAAAIAWPLLKRARGRLLI
                     VVLAAAILGGAAGMAVARLVPVHRPVVTRKTVGTPFWEVAANAGKKCRIIRVPAMFPP
                     DPVKGGEIHAGLGVPDIRGTNGNYTYYTTETMSLGGANTEKGGRIENVAFLDGHTTTT
                     LYGPRNRLFDDPPDITLPLELKLRREAGDRGVDITVGGKTMRLPEGAWSDWVELEFAF
                     NPLIRAHGLARFYLQSVE"
BASE COUNT          301 a          642 c          674 g          296 t
ORIGIN      
        1 cggcaccgca aaggtccacc tggagcatga gggggcccgc caccagtcgg tcgatctggg
       61 ccgccgtcgc ccggactcga accgtcacgc tgtccgggag ggctccccgg cccgggtcgg
      121 ccgaggcgca gcgccaggcc agcggcaccc ggaggtcgat cgtccgctcg gtttcgcggt
      181 gaacgtggag atagagggcc gtggcgagga agatggccgc cagcttgagc cccaggtcac
      241 ggaagaggcg gcgggcgatg gtgttgagga gcgccatcag gccccggtca tggggtgacg
      301 atgatcacgc cgagaaagtc gccggtgtac ccgtccggca cttcgcggct gaagtagtag
      361 aaggtgtcgg gttcggtgaa ctggcgctgg agtcggccgc cgatggcctc cccggaggga
      421 tagccggcca gcgagacgtc gaggatttca ccggcagcgg gatcctcggg actgacaccc
      481 gaggtgaccg tccgcgtgtc gcgtgaccag ttctgccact cgacccgctg gttcacttcg
      541 atggtgacgt tggacgggat gtaggtcgtg tcgcggatct tgatcaccac atcgatgtga
      601 ggtccggtgg gtgggtcttc gctgcagccg ggcgccgcga ccagggtgac aagggcgggt
      661 ccgatgacca gcaacgtggc ggggagcgtg aggcggaacg ggtgtcgatg aacggacatg
      721 tggactcctc gggcgggatc gcggcagcgg aatggcaccc tcccccatgt tagactcggc
      781 cgccgtgtct gaccagtcgt cgagcgccaa agtccccggg cgcgaacctg attcgatcgt
      841 cggaggttct ccgccccatg cctgaattcc gcggcccggc agccgtactg cggcctgtcc
      901 gggcgctcgt cgccctgttc gtcctgctca tgccggtgat ctttccagcc cccgcctccg
      961 cacgtgtcgt ggtgctcggt ttcgatggag cggatgcccg cctggtcgag cgatggatga
     1021 acgagggaaa gctccccaac ctggaccgcc tgcgccggga aggaacctgg tcgccgctcg
     1081 gcacgaccac gcccccccag acgcccgtgt catggacctc ctttgccacc ggcatcaacc
     1141 cggggcggca cggcatcttc gacttcctct cgcgtgacgt ggccacctac cagcccggct
     1201 acgcgatgtt cgacatccgc gacgtgaagg tgggcgcggg aaaggccaac ggcccgctgg
     1261 ccggcggcct ggcagggctc ctcgcggcgg ccatcgcgtg gccgctcctc aagcgtgcca
     1321 ggggacggct cctgatcgtg gtcctggcgg cggcgatcct gggtggcgcg gccggcatgg
     1381 ccgtggcgcg actggtgccg gttcaccggc ccgtcgtcac ccggaagacg gtcggtaccc
     1441 cattctggga agtggcggcc aacgccggga agaagtgccg catcatccgt gtgccggcca
     1501 tgttcccgcc cgacccggtc aaggggggcg aaatccacgc cgggctcggt gtgccggaca
     1561 tccgcggcac caacggcaac tacacgtact acaccacgga gacgatgagc ctggggggcg
     1621 ccaacaccga gaagggtggc cgcatcgaga acgtggcctt cctcgacggc cacaccacca
     1681 ccacgctcta cgggccgcgc aaccgcctct tcgacgaccc gcccgacatc acgctgccgc
     1741 tcgagctcaa gctccgccgc gaagcgggcg atcgcggcgt ggacatcacg gtcggcggaa
     1801 agacgatgcg gctgcccgag ggggcctgga gcgactgggt ggaactcgag ttcgccttca
     1861 atcccctgat cagggcccac ggccttgccc gcttctacct ccagtcggtg gag
//
LOCUS       DASVOD010000110         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_633108, whole genome shotgun sequence.
ACCESSION   DASVOD010000110 DASVOD010000000
VERSION     DASVOD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633108"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..986)
                     /locus_tag="VF720_02155"
     CDS             complement(<1..986)
                     /locus_tag="VF720_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX7878180.1"
                     /translation="MEPMEAVQLLPNWLRFLTLQDANVRWVLFGSILLGAGSGVLGCF
                     AFLRKQALLGDALAHAALPGVCLSYMLFQSKNPAVLLAGAMLTGWLALRAIHTSVANT
                     RIKEDTALALVLSTFFAAGIVLLTRIQQSGAASQAGLDKFLFGQAAALVERDVIVLGG
                     LAVALVAAVVIAFKEFKVCAFDAGYAQTIGINVKLVDGLLAILIVAAVGIGLAAVGVV
                     LMAAMLVTPAAAARYWTDRLSRMVLLAGLFGAFSGALGTFISFLAPRMPTGPWMVTAV
                     TTMFAVSLIFAPRRGLLARGLLHWRNRRRTAEENLLTTLYRLDEKAAASAWP"
     gene            complement(996..1775)
                     /locus_tag="VF720_02160"
     CDS             complement(996..1775)
                     /locus_tag="VF720_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878181.1"
                     /translation="MSAEPPIEVHNLTVAYGRRPVLWDVDFAVPAGQLIALVGPNGAG
                     KSTLIKAIMGLLPLSSGWVRVFGEPLEKRRRDVAYVPQRESVDWDFPTDALDVVMMGR
                     YGHLPIFGRPGRDDRRVALEALDKVGMSAFARRQISQLSGGQQQRVFIARALAQDASI
                     YLMDEPFAGVDAATESAIVQLLRDMKTRGKTVLVVHHDLQTVPDYFDWTMLLNMRMVA
                     FGPTPQVFTQERLNETYGGRLNLLTQVTERIQREEARRRTL"
     gene            complement(1772..2797)
                     /locus_tag="VF720_02165"
     CDS             complement(1772..2797)
                     /locus_tag="VF720_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEX7878182.1"
                     /translation="MLAITCWSQRHRTSGLLSVLLALLFAGCGGAPAGRNENGGVAAT
                     GASSHGGSVSTLVRPSDASGPITITATTGMVAALAAEVGGSRVAVQALMGPGVDPHLY
                     KATQGDLEKLSNAEIIFYSGLHLEGKMVDIFERMSRTRPTVAVTHLLDEARLRRPPEF
                     AGNFDPHVWFDVAMWSDCSKAVEAELARYDPANAAEYQANGAALREGMATLDAWCREQ
                     IATIPREQRLMVTAHDAFGYFGRAYDIEVLGLQGISTVAEYGLQDLTRMVDTIVARKV
                     RAVFVESSVPKKSIESLVSGCRDRGHPVTIGGQLFSDAMGAAGTPEGTYDGMVRHNVT
                     TLVGALR"
     gene            complement(2803..3495)
                     /locus_tag="VF720_02170"
     CDS             complement(2803..3495)
                     /locus_tag="VF720_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX7878183.1"
                     /translation="MFAASHISVAQEDYIRTISKLYNGLDPVGVTELAQALGVTPPSV
                     SGMIQRLEAEGFVEHRPRHGVTLTEAGQAAALNIHRRHRLIETFLVQALGLDWAEVHE
                     EAEALEHHLSERVVRAIDRYLGHPTTDPHGHPIPAADGSMLIRDLTPFEMLAAGDAAT
                     IREIGSDDPARIRLWKSLGLVPGAEVTVRERLELEGIWRLDVEGREVITGRPGIEGLK
                     VERMPPASHIGG"
     gene            3606..>4114
                     /locus_tag="VF720_02175"
     CDS             3606..>4114
                     /locus_tag="VF720_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008356157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7878184.1"
                     /translation="MDERFAAAFVAAYDFPDSESLVLGAPFQAGETATGTLIRQPLAM
                     MNRHGLIAGATGTGKTRTLQMLAGQLSAAGVPVFLADMKGDLSGLTTPGAVTPKIDER
                     CAAIGRPFQPAGCPVEFYSLTGKGGLPLRATVSSFGPLLLSRVLDLNETQSSALAMVL
                     KFCDDQGLLL"
BASE COUNT          690 a         1397 c         1369 g          658 t
ORIGIN      
        1 atcggccagg ccgatgccgc cgccttctcg tcgaggcgat agagggtggt caggagattc
       61 tcctcggccg tgcgtcgccg gttgcgccag tgcaggagcc cccgggcgag cagaccacgg
      121 cgtggtgcga agatgagcga aacggcgaac atggtcgtga cggcggtcac catccacggg
      181 ccggtcggca tgcggggggc gagaaaggaa atgaaggtgc cgagcgcgcc cgagaaggcg
      241 ccgaacagcc cggccaggag caccatccgc gagagccggt cggtccagta gcgtgcggcg
      301 gctgccggcg tcaccagcat ggcggccatg agcaccactc ccaccgcggc gaggccgatg
      361 ccgacggcag ccacgatcag gatcgccagg aggccgtcga ccagtttcac gttgatgccg
      421 atcgtctgcg cgtaacccgc gtcgaacgcg cacaccttga attccttgaa ggcgatcacg
      481 acggccgcca ccagggccac ggccaacccc cccagcacga tcacgtcgcg ctcgacgagc
      541 gccgccgcct ggccgaagag gaacttgtcg aggcccgcct ggctggccgc accactctgc
      601 tgaatccggg tgagcagcac gatgcccgcc gcgaagaacg tggaaagcac gagggccagc
      661 gcggtgtctt ccttgatccg ggtgttggcc accgacgtgt ggatggcgcg aagggccagc
      721 caccccgtga gcatggcccc cgccaggagc accgcagggt tcttgctctg gaagagcatg
      781 tagctgaggc agacgcccgg gagcgccgcg tgtgccaggg catcgcccag caacgcctgc
      841 ttccggagga acgcgaagca accgagcacg ccgctcccgg cgccgagcag gatggagccg
      901 aacagcaccc agcggacgtt ggcgtcctgc agcgtgagga atcggagcca gttggggagg
      961 agctggacgg cttccatcgg ttccatcgcc cgctcctaaa gggtccggcg tcgcgcttct
     1021 tcgcgctgga tccgctccgt gacctgggtg aggagattga gtcgcccacc ataggtctcg
     1081 ttcaggcgct cctgcgtaaa gacctgcggc gtgggaccga aggccaccat gcgcatgttg
     1141 aggagcatcg tccagtcaaa gtagtccggt accgtctgca ggtcgtgatg caccacgagc
     1201 accgtcttcc cccgggtctt catgtcgcgg agaagctgga cgatggccga ctcggttgcc
     1261 gcgtccacgc ccgcgaacgg ctcgtccatc aggtagatgg aggcgtcctg tgccagggcc
     1321 cgcgcgatga agacgcgctg ctgctgcccc cccgagagct ggctgatctg gcgccgggcg
     1381 aaggcgctca tccccacctt gtccagggcc tcgagcgcca cgcgccggtc gtcgcgcccc
     1441 ggccggccga agatcggcag atggccgtag cgccccatca tgaccacgtc cagggcatcg
     1501 gtgggaaagt cccagtcgac cgactcgcgc tgcggcacgt aggccacgtc gcggcgccgc
     1561 ttttcgagag gctcaccgaa gacgcgcacc caccccgatg agagtggaag gagccccatg
     1621 atggccttga tgagggtgga cttgccggcg ccgttcggcc ccaccagcgc gatgagctgc
     1681 ccggccggca cggcgaagtc gacgtcccac agcaccggtc ggcggccgta ggccacggtc
     1741 aggttgtgca cttcgatcgg cggttcggcg ctcaccggag ggcccccacg agcgtcgtca
     1801 cgttgtggcg taccatgccg tcataggttc cttccggcgt gccggccgcg cccatggcat
     1861 ccgagaagag ctgcccgccg atggtgacgg gatggccgcg gtcgcggcat cccgacacga
     1921 gcgattcgat cgacttcttc ggaacgctcg actcgacgaa gaccgcccgt accttccgcg
     1981 ccacgatcgt gtcgaccatg cgggtcaggt cctggagccc gtactcggcc acggtgctga
     2041 tcccctgcag gccgagaacc tcgatgtcat aggcccggcc gaagtagccg aaggcgtcgt
     2101 gcgccgtgac catgaggcgc tgctcgcgcg ggatcgtggc gatctgttcc cggcaccatg
     2161 cgtcgagcgt ggccatcccc tcgcggagag ccgccccgtt ggcctggtat tcggcggcgt
     2221 tggccggatc gtaacgggcc agctcggcct cgacggcctt cgagcagtcg ctccacatcg
     2281 ccacgtcgaa ccagacatgg ggatcgaaat tgccggcgaa ctcgggcggg cgccggagcc
     2341 gtgcctcatc cagcagatgg gtgacggcca ccgtggggcg ggtgcggctc atccgttcga
     2401 agatatccac catcttcccc tcgaggtgga gcccgctgta gaagatgatc tcggcgttcg
     2461 agagcttttc caggtctccc tgggtggcct tgtacagatg gggatccacc cccggtccca
     2521 tcagcgcctg gaccgccacc ctggagcccc ccacctcggc ggccaatgcg gccaccatgc
     2581 cggtggtggc cgtgatggtg atggggccgg acgcgtcgga gggacgcacc agggtcgata
     2641 cggagcctcc gtgcgacgaa gcaccggtag cggcaacgcc gccgttctcg ttgcggccgg
     2701 ccggtgcgcc gccgcatccg gcaaacaaaa gagccaggag cacggacagg agtcccgatg
     2761 tccgatgccg ttgtgaccag caggtgatcg cgagcacgtt gttcaacctc cgatgtggga
     2821 tgccggcggc atccgttcca ctttcaagcc ttcgatgcct ggccgcccgg tgatgacctc
     2881 acgcccttcc acgtcgagcc gccagatccc ttcgagctcc agtcgttccc gcaccgtgac
     2941 ctcggcgccc ggaaccagcc cgagcgactt ccacagccgg atgcgggcgg gatcgtccga
     3001 gccgatctcc cggatggtgg ccgcatcccc tgcggccagc atttcgaacg gggtgagatc
     3061 ccggatcagc atgctgccgt cggccgccgg gatgggatga ccgtgcgggt cggtggtggg
     3121 atgaccgagg tagcggtcga tggcccgcac gacccgctcc gacaggtgat gctcgagcgc
     3181 ctcggcttcc tcgtgaacct cggcccaatc gagcccgagg gcctgcacga ggaatgtttc
     3241 gatcaggcga tgacggcggt ggatgttgag tgccgcagcc tggccggctt ccgtcagcgt
     3301 gaccccgtga cgcggccggt gttcgacgaa tccttccgcc tcgaggcgct ggatcatccc
     3361 gctgaccgat ggcggcgtga cgccgagcgc ctgcgccagc tcggtgactc ccaccggatc
     3421 caagccgttg tagagcttgg agatggtacg gatgtagtct tcctgtgcca cgctgatgtg
     3481 agatgccgca aacatggaaa ttaggataac ctaatttgtg atgacgtcaa gacaatattt
     3541 ccaggtgttg gcctcgaagc aggcaaccac tgagccccgt cccgcacccc tgctagactg
     3601 cggccatgga tgaacgattt gcggccgcct tcgtggcggc ctacgacttt cccgatagcg
     3661 agagcctggt tctcggcgcc ccgttccagg cgggcgaaac cgcaaccggt accctgatcc
     3721 gccagcccct cgccatgatg aaccggcacg gcctcatcgc cggggccacc ggcaccggaa
     3781 agaccaggac tctccagatg ctggcgggcc agctctcggc agccggggtg ccggtcttcc
     3841 tggccgacat gaagggtgac ctgtccggac tcactacccc cggcgcggtc actccgaaga
     3901 tcgacgagcg ctgtgccgcg atcggccgcc ctttccagcc cgccggttgc ccggtggagt
     3961 tctacagcct gaccggaaaa ggcgggttac cactccgggc taccgtttcc tcgttcgggc
     4021 cgctcctcct ctcgagggtg ctcgacctca atgaaaccca gtcgagcgcg ctggcgatgg
     4081 tcctcaagtt ctgcgacgac caggggctcc tcct
//
LOCUS       DASVOD010000111         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_320275, whole genome shotgun sequence.
ACCESSION   DASVOD010000111 DASVOD010000000
VERSION     DASVOD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320275"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2184)
                     /locus_tag="VF720_02180"
     CDS             complement(<1..2184)
                     /locus_tag="VF720_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878185.1"
                     /translation="MVTLNYARMRRGVLRFVLLGGILATGSAPCAARPISDDHGYRIE
                     EIQVQNDVRDAGGAPLSSTLFQDTVSYGGTFWAPDSMRWEAIRDSCWTFDSGVGSSLN
                     AGANPNKPVGYHETMEGWTGLDQSLNPVPYFRRSSTCVINGSYSLWAGVSLAEANSLC
                     FASGQGYGNSWSMTVSKTFNYPGSGSVTLAWKYAIDAEPGFDYAYVRVDTTGSGAAPD
                     IELASYNGVASGMGSIVLSPGVRMRSSAGAIRIKFVAEADGGYSDEDGAYPTTCGLAV
                     FDDITLSGAITEVTNFEADMNGWAQEIPVIGVGDFSHLADVADLPPAATFCPCNIRDS
                     VLVFSDLVDQHPIDQDNVAVSPWIDLLAGGDVGRPGKLILYSLYGVLPITDYIFIQVR
                     VRYYPEVCPSTGLLYRTPFKDQNTLFPTTSFCTPVGSTNARDFSAVFSPGAEQAQMAL
                     GVISLCRTAPFGPVCSGVTNSTPWFDHVSLGVYSNATVPTVNTTTFDRYQDNFAADGT
                     LNPASPGRLDQNRIKGASTPSSGTILGDTLVARGNGGNTETRLVFRVRPGPFVNNAAL
                     SSMVARWTYEPSLNGKYGTDGNPATWPNWYSARLDTAEQAGVVQTSPFTWMSTFHEAD
                     PGFQGTDRTADPNDPGRLANDILPDHIFTPGSRIDYFIAARYRPPDPRNPGGNNWYLD
                     PDTANGRFREVEILPSSMAADTSWNCVLYVDHHDDRDGEDQYLEDL"
BASE COUNT          417 a          737 c          735 g          385 t
ORIGIN      
        1 gaggtcttcg aggtactggt cctcaccgtc ccggtcgtca tggtgatcga catacagcac
       61 gcaattccat gacgtgtccg ccgccatcga cgacggcagg atctcgacct cgcggaagcg
      121 gccgttggcc gtatcgggat cgaggtacca gttgttgcca ccgggattgc gtggatccgg
      181 gggccggtag cgtgccgcga tgaagtagtc gatgcgcgat cccggcgtaa agatgtgatc
      241 cggaagaatg tcattggcca ggcgcccggg atcgttcgga tcggcggtcc ggtccgttcc
      301 ctggaagccc ggatcggcct cgtggaaagt ggacatccag gtgaacggcg aggtctgcac
      361 cacccctgcc tgctcggccg tatcgaggcg cgccgagtac cagttgggcc atgtcgccgg
      421 attgccatcg gtaccgtatt tgccattcag gctgggctcg tacgtccagc gggccaccat
      481 cgacgagaga gccgcgttgt tcacgaaagg accgggacgc accctgaaca cgaggcgcgt
      541 ctccgtgttc cccccgttgc cgcgggccac cagggtatcg ccgagaatcg tgccggatga
      601 cggtgtcgag gcccccttga tgcggttctg gtccagtcgc cccggcgagg ccgggttcag
      661 cgtcccgtcc gccgcgaagt tgtcctgata gcggtcgaac gtggtggtgt tgacggtggg
      721 tacggtcgca ttgctgtaga cgccgagcga gacgtgatcg aaccaggggg tggaatttgt
      781 cacgccagaa cagacggggc cgaacggcgc ggttcgacag aggctgatga cgccgagcgc
      841 catctgcgcc tgctccgcac cgggactgaa caccgccgag aagtcgcggg cgtttgtcga
      901 gccgacgggt gtacagaaac tggtggtggg aaacagcgtg ttctggtcct tgaacggcgt
      961 gcggtaaagc aggccggtgg acgggcacac ttccgggtag tagcgaacgc gcacctgtat
     1021 gaagatgtag tcggtgatcg gcagcacgcc gtaaaggcta tacaggatca gcttccccgg
     1081 acgccccacg tccccgcccg ccagcaggtc gatccacggc gacaccgcca cgttgtcctg
     1141 gtcgatgggg tgctgatcca ccaggtcgga gaagacgagc accgagtcac ggatattgca
     1201 gggacagaag gtggccgcgg gtggcagatc cgccacatcg gccaggtggc tgaagtcccc
     1261 gaccccgatc acggggatct cctgcgccca cccgttcatg tccgcctcga aattcgtgac
     1321 ctcggtgatc gccccgctca gggtgatgtc atcgaacacc gcgagcccgc aggtggtggg
     1381 atacgcccca tcctcgtccg aatagccgcc gtcggcctcg gccacgaact tgatgcgaat
     1441 ggcgccggcg ctcgaacgca tgcggacccc cggcgagagc acgatactcc ccatcccgct
     1501 cgcgacgccg ttgtacgaag ccagctcgat gtcgggcgcg gcgccggagc cggtggtatc
     1561 gaccctcaca tacgcgtaat cgaatccggg ctcggcatcg atcgcatact tccatgccag
     1621 cgtgacgctc ccggaacccg gataattgaa ggtcttcgac accgtcatcg accaggaatt
     1681 gccgtacccc tgccccgatg cgaagcagag cgaattggcc tcggccagcg aaacgccggc
     1741 ccacagcgag tacgacccgt tgatgacaca ggtcgaagag cgcctgaagt agggcaccgg
     1801 gttcagcgac tggtcgagcc cggtccatcc ctccatcgtc tcgtggtagc cgaccggctt
     1861 gttcgggttg gcccccgcgt tgagcgacga cccgacgccg ctgtcaaagg tccagcacga
     1921 gtcgcggatg gcctcccatc gcatcgagtc gggcgcccag aatgtaccgc catagctgac
     1981 cgtgtcctgg aagagggtgc tcgagagcgg cgcgcccccc gcatcccgca cgtcgttctg
     2041 aacctgtatt tcctcgatgc gataaccatg gtcatcggag atggggcggg ccgcgcaggg
     2101 agccgagccg gtcgccagga tgccgccgag caagacgaag cggagaaccc ctcgacgcat
     2161 gcgagcgtag ttcagtgtca ccacgatgcc tccttgagcg atctgccgac agactggagc
     2221 cgacctactc cccctccacc accatcaact tccgcgcctc cacccgatcc cccg
//
LOCUS       DASVOD010000112         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_8127, whole genome shotgun sequence.
ACCESSION   DASVOD010000112 DASVOD010000000
VERSION     DASVOD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8127"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..740)
                     /locus_tag="VF720_02185"
     CDS             complement(237..740)
                     /locus_tag="VF720_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7878186.1"
                     /translation="MDWKQDLIAEFDQEFAGTRRMLERVPEEKFDWKPHPKSMSIGEL
                     AAHLVSIPAWAAYTVRQSSFDVAPVGGEAYRDEPLATRAAVLETFDRNAAEARSVLAD
                     LTEADMDAPWSLLGGGQLYFTRPKGKVLRDFIFSHNTHHRAQLGVFLRLNDIPVPGVY
                     GPTADEQ"
     gene            complement(755..>1959)
                     /locus_tag="VF720_02190"
     CDS             complement(755..>1959)
                     /locus_tag="VF720_02190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7878187.1"
                     /translation="RLILHLDIDAFFAAIEQQLNPRLRGRPVIVGNGVIASCSYEART
                     FGCYAGQRLSEARRMCPGVVILDGCEKTYRAFAARIFELAREFSPAVEAYLDEAYCDL
                     TGTDRLYGGDWAAAARELKRRIVERTGLTVTMGLGTSRMVAKMASKAVKPDGLHRVAA
                     GEEEPFIRALAIEKLPGVGRSTAEVLRRFNIRTIAELRVLSIDHLVTLFGEPGRLLYE
                     RCRGRDTQPVTVREVPQSISRETSFHEATIDPGEISGMLYYLTERAVKTARQLGLKSK
                     TVTVKVRYTDSGAPAMSRSLAAASDRDEEIFALAVDLLARLHTRRESLHGIGMTLSGF
                     VLDGAGQLDLFDPERGERLDRLYASLDRVRDRFGHSAVVAGRSIALMGKLEQDGYGFV
                     LRTPSLTR"
BASE COUNT          301 a          700 c          663 g          295 t
ORIGIN      
        1 ggtagaggtg ggcgccccga tgccggtggc cctcggagcc acgaggtagc cggccggggc
       61 ggtcggcgtg gcggcggccg gcggggcgcc cggcccggca aggaggagag cgacggccat
      121 gaggcagcag gcggaaaagc agcgaagaag tcggcccatc ggccaacaag attatcacag
      181 gtccggagga cgcccggcgc cgttggcggc gggcgtcccc ccgatccctc gcttcgctac
      241 tgttcgtcgg ccgtcgggcc gtaaacgccg ggcactggaa tgtcgttcag ccgcaggaag
      301 acgccgagct gcgcccggtg atgcgtgttg tggctgaaga tgaaatcgcg cagcaccttc
      361 cccttcggcc gcgtgaagta gagctgcccg ccgccgagca gcgaccaggg cgcgtccatg
      421 tcggcctcgg tcaggtcggc cagcaccgac cgcgcctcgg ccgcgttccg gtcgaacgtc
      481 tcgagcaccg cggcgcgggt ggccagcggc tcatcgcggt aggcctcgcc gccgaccggc
      541 gccacgtcga acgacgactg gcggacggtg tacgccgccc acgccgggat gctcaccagg
      601 tgggccgcca gttccccgat cgacatcgac ttcggatgcg gtttccagtc gaacttctcc
      661 tcgggcaccc gttcgagcat cctccgtgtg ccggcaaact cctggtcgaa ctcggcaatc
      721 aggtcctgct tccagtccat gggcttctcc ttgtctatcg ggtcaacgac ggcgttcgca
      781 gcacgaaacc atatccatcc tgctcgagtt ttcccatcag ggcgatcgac cgccccgcca
      841 ccacggccga gtgtccgaag cggtcgcgca cgcggtcgag gctggcgtac aggcggtcga
      901 ggcgctcccc ccgctcggga tcgaacaggt cgagctgccc ggcgccgtcg agcacgaagc
      961 ccgacagcgt catgccgatg ccgtgcaggc tctcccgccg cgtgtggagc cgcgccagca
     1021 ggtcaaccgc cagcgcgaag atctcctcgt cgcgatcgga ggccgccgcc agcgagcggc
     1081 tcatggccgg cgcgcccgaa tcggtgtagc gcaccttgac cgtcaccgtt ttcgatttca
     1141 ggccgagctg ccgggccgtc ttcaccgccc gctcggtcag gtagtagagc attcccgaga
     1201 tctcgcccgg atcgatggtg gcctcgtgga acgacgtttc gcggctgatc gactgcggca
     1261 cctcgcgcac cgtgacgggc tgcgtgtccc gcccccggca gcgctcgtac aacagccgcc
     1321 ccggctcgcc gaagagcgtg acgagatggt cgatggacaa gacgcgcagt tccgcgatgg
     1381 tgcggatgtt gaaccgccgg agcacctcgg cggtggagcg ccccacgccc ggcagcttct
     1441 cgatggcgag cgcccggatg aacggctcct cctcccccgc cgccacgcga tggagcccgt
     1501 cgggcttgac cgccttcgat gccatcttgg ccaccatgcg gctcgtgccc aggcccatcg
     1561 tgacggtgag ccccgttcgt tcgacgatgc ggcgcttgag ctcccgtgcc gccgccgccc
     1621 aatccccccc gtacagccgg tcggtgccgg tcaagtcgca gtaggcctcg tcgagatacg
     1681 cctcgaccgc cggcgagaat tcgcgcgcca gctcgaagat gcgggcggcg aaagcgcgat
     1741 aggtcttctc gcacccgtcg aggatgacga cgcccgggca cattcgccgc gcctccgaca
     1801 gccgctgccc cgcgtagcag ccgaacgtgc gcgcctcgta cgagcacgac gcgatgacgc
     1861 cgttccccac gatgaccggc cggcctcgca gccgcgggtt caactgctgc tcgatggccg
     1921 cgaagaaggc gtcgatgtcg aggtggagga tgaggcggt
//
LOCUS       DASVOD010000113         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_999378, whole genome shotgun sequence.
ACCESSION   DASVOD010000113 DASVOD010000000
VERSION     DASVOD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999378"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(620..2137)
                     /locus_tag="VF720_02195"
     CDS             complement(620..2137)
                     /locus_tag="VF720_02195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7878188.1"
                     /translation="MEFLQVNTYQYVTSAVHVEELDDLCWARLAERTDMAELSSVAGR
                     SDILVIGGGLIGCSIALECARRGRTVTVLEKLDQVGQGSTARSTAIIRQLYHETHSIK
                     LAIEGLRLWEQWSKHTGHDVELVRFHQPGILWVVKPEALATMPVRTMKELGAEVEVMD
                     EPSMTRRFPSYRFSGPLAGSTVCGLFEKRGGYVEDPSRAVRNIAEAARRAGVRFHTGE
                     TVTSVEHDDAGCGAGTGGAGAGGAGAGGAGAGGAGAEGAGAEGAGVGGTSAASARPRR
                     VTAIITSSGRRFEADTFVNAGGPHSAAVNTMAGCPMPLATAPNRQQFVEGRETALDWN
                     GARDGSYPVCADPVSGIYFRPSRTQFRVGTVLAEDDRHFVTDADAPLPEPTPEFITDK
                     LERLRRRQPGARVLDVRGTSALYDVTLADWCPILDKSDLDGWFVAIGTSGKWFKSGPV
                     IGWIMAELIEWVRTGKDPDKPTDRLTLRLPRTGHELALADFARWRKPHGTEGVLA"
     gene            2247..>2628
                     /locus_tag="VF720_02200"
     CDS             2247..>2628
                     /locus_tag="VF720_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7878189.1"
                     /translation="MTPRSDPHNCDVAVVGAGAAGFMAAIAAAEAGARTTLFNAHLLP
                     GLKILMSGGTRCNVTHVTVGPESFHAASRPAVGLVLKTFPAGAVRAWFEEQGVPLKEE
                     PGGKLFPVSNSARTVVERLLATASG"
BASE COUNT          448 a          834 c          869 g          477 t
ORIGIN      
        1 ggccggtcgg ggtgttgatg aagcgggtca tgacgtcctc cttgagaaga ttccgttggc
       61 ttcggctgcg ggtcttcgtt gcgttgcccg cgtcggatga tgcggacggg aacaggtcaa
      121 agcagcgatt gtgccaatgg ccggggaggg caatgcaaca ccggtaaaac gatgtcttga
      181 tagaagatat gggtgagtgt cggatggggt tgggtgggcg gtggtgcgga gaacgttaac
      241 ttgtattaac gcgaatagcg gtggtcggcg cggggggtta acaggttaac gtgcgacctg
      301 gtttgcgatc aggtccgatt gaccggactg agatcagttc gagcgccccc ttccgactgc
      361 gccgttcctc tctgggagaa cctgttccgg aacaggtagc gaatttcgtc atcggaagag
      421 atcgccgttc cgctctggga gaacctgttc cggaacaggt aacgagtgtc gtcacaagaa
      481 aaggactgtg gcgactcctg gcatcgtcgc gtgcgatcga tgcgacttgc gtcaagaggg
      541 cgactatcac gggtcaagct tgccgcacgt actgattgag aaacgccaca ccgcggtacc
      601 gatgcggcga tcacctggat caggccagca cgccctcggt cccgtggggt ttccgccagc
      661 gggcaaagtc ggccagtgcg agctcgtggc cggtgcgcgg gagacggagg gtgagtcgat
      721 cggtcggctt gtcggggtct ttgcccgtgc ggacccactc gatgagttcc gccatgatcc
      781 agccgatgac cgggcccgac ttgaaccact tgccgctggt gccgatggcc acgaaccagc
      841 cgtccaggtc ggacttgtcg aggatcgggc accagtccgc cagagtcacg tcgtacagcg
      901 ccgaggtgcc acgcacatcg aggacgcgcg cgcccggctg gcggcggcgc agtcgttcga
      961 gcttgtcggt gatgaactcg ggagtgggct cggggagggg agcgtccgcg tcggtcacga
     1021 agtgacgatc gtcctcggcg agcacggtcc ctacgcgaaa ctgcgtgcgt gacgggcgga
     1081 agtagatgcc gctgacgggg tcggcgcaaa ccggatagga accgtcccgg gcgccgttcc
     1141 agtcgagagc ggtttcgcgc ccttccacga actgctgccg attgggtgcg gtggccagcg
     1201 gcatcgggca ccccgccatc gtgttgacgg cggcggaatg tggcccgccg gcattgacga
     1261 acgtgtcggc ctcgaagcgc cgaccgctgc tggtaatgat ggcggtcact cggcgagggc
     1321 gcgcagacgc cgccgacgtc ccaccgaccc ctgccccttc ggcacccgcc ccttcggcac
     1381 ccgccccacc ggcacctgcc ccaccggcac ccgccccacc ggcacccgcg ccaccggtac
     1441 ccgcgccaca acccgcatca tcatgctcga ccgacgtcac cgtttcgccc gtatgaaaac
     1501 gcaccccggc gcgtcgagcc gcctcggcga tgttgcggac ggcacgggaa ggatcctcga
     1561 catagccgcc ccgcttttcg aacaggccgc agacggtgga gccggccagc gggccggaaa
     1621 agcgatagct gggaaagcgt cgcgtcatcg atggctcgtc catcacctcg acctcggcgc
     1681 cgagctcctt catcgtgcgc accggcatgg tggccagtgc ctccggcttc acgacccaga
     1741 ggatgcccgg ctggtggaac cggacgagct cgacatcgtg gcccgtgtgc ttcgaccatt
     1801 gctcccagag tcgaagtccc tcgatggcca gcttgatcga gtgcgtttcg tggtacagct
     1861 gtcggatgat ggcggtggag cgagccgtcg acccttgccc tacctggtcc agcttttcga
     1921 ggacggtcac cgtgcgaccg cgccgcgcgc attccagggc gatggaacag ccgatcaggc
     1981 ccccaccgat caccaggatg tccgatcgcc ccgccacgct tgaaagctct gccatgtctg
     2041 ttctctccgc caaccgggcc caacagagat cgtcaagttc ctcgacatgt actgcgctgg
     2101 taacatattg ataggtattt acttgcagga attctatatg cgaaacagct cacgttgcca
     2161 gtcaattatt cttccgatcg cggattatgc gcgctcgcgt cgatccccgg acatgccctc
     2221 ttgcgacctt cggctatcct gcgcgcatga cgccacgttc cgatccccac aactgtgacg
     2281 tagccgtggt gggtgccggc gccgccgggt tcatggcggc gattgcggcg gcggaggcgg
     2341 gcgcgcgcac gaccctcttc aacgcccacc ttcttccggg actgaagatc ctcatgtcgg
     2401 gcggcacgcg atgcaacgtg acgcatgtca cggtggggcc ggagtcgttt cacgccgcat
     2461 cgcggccggc ggtaggcctc gtcttgaaga cgttccccgc aggcgcggtc cgtgcgtggt
     2521 tcgaggagca gggtgttccc ctcaaggaag agcccggcgg caagctcttc cccgtgagca
     2581 actccgcgcg cacggtggtc gagcggttgc ttgcaacggc ttccggcg
//
LOCUS       DASVOD010000114         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_59838, whole genome shotgun sequence.
ACCESSION   DASVOD010000114 DASVOD010000000
VERSION     DASVOD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59838"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF720_02205"
     CDS             complement(<1..224)
                     /locus_tag="VF720_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020126113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7878190.1"
                     /translation="MTDSRPAGGAGLLAGRLAFITGASSGIGAATARAFHREGARLVL
                     AARRLERLEALADELRAASGVSGTDRRGAS"
     gene            complement(221..1708)
                     /locus_tag="VF720_02210"
     CDS             complement(221..1708)
                     /locus_tag="VF720_02210"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7878191.1"
                     /translation="MKELTMMRSNETTRRFRAGAMLVMAAGLFAANPVAPATAATPAR
                     SWKDVKITPLEWKKPEPTTIKLRNGVTVYLMEDHRLPLISFYGTVRTGALYDPPGKAG
                     TAGLMGNLLRTGGTAKRPWDQVDAEVDRLAMSVSTGVGNESGNASFQVLTENFQPALN
                     LLFEMLREPAFDAEKLALAKEKIKDGIRRQNDNPVQIALRELPRRLYGTDHPRGFAPT
                     FATVDAITRQDLVDFHKKYYVPSNFLIGVAGDFDPKTIAATIEAAMGSWPDATVELPS
                     VPPVPMNTPRAIFQADKTSATQSTILISRLGTKVGDPDAAALEVMDFILGSGGFTSRV
                     VEAVRSDEGLAYASGSALQLGNLDPGPEIVYAISKGESTVKALEIMLREIGRMRDEPV
                     TKAELSRATNGLLNAEVFNYDQPDKVLASVLDLAYYGLPQDLPTKRLEALAKVSAEDV
                     QAMARKYLPDDQLQILVVGAAANFDKPLSSIGPVTTIELKDPTAP"
     gene            complement(1705..>3793)
                     /locus_tag="VF720_02215"
     CDS             complement(1705..>3793)
                     /locus_tag="VF720_02215"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7878192.1"
                     /translation="NGMTILMVENHQSPTIGLIVQFAVGSVDEWDGISGSAHILEHLL
                     FKGTADMGSTDWKKEKLLLDRIETTAQDLRLERGKESRADNGRLLMLQTELDSLQKAA
                     RGYVEPNPYDRVYTENGSQGFNAGTSWDGTNYEIALPSNRLELWMKIESDRLKAPVLR
                     EFYTELGNIMEERRLRTDDQPAGPLGKVGEAIYATAYTAHRYGVPVIGWPSDIQQVTR
                     TEVEAFFKKYYAPNRVTLGIVGDIDPDKVTEMAKAYFGSIPRQPDPIPPRTVEPMQKG
                     ERRFLVEYDAETRVLAAWHITDGHHPDYPALLMMEDILTGGRSSRMINNVVEKQKIAS
                     SIASYTGIPGERYPGLFILEATPLPPHTTQEVEAAMYAEITRLKKEPPTEAELAAAKL
                     RYRKQFVNGLEDNLGLATALAYNNATLGDWRDSFRRAEAVANVTPADIQRVASTYFNA
                     SNRTVGTLVKPTPEAAFVDPKAEAEAMEILGKARSSFGKEATLAGLKDLTVESTLTIF
                     AQGQAIPASQKQTFTIDGRMKTELSIMGQAQVQTLDGETGWITSPGGMQDADAAALSD
                     MKEGFTREVYLLKHVGQNPNGTARRLPDGDFQGKPSIIIEITPVEGRPFNLYLDPATN
                     RPRGRSFDTENPLTGQPGRAEEALLDWQEVSGVWWPAKEEIWMGGEKILEKLTTKRTV
                     NSGVTPAEFRKPS"
BASE COUNT          533 a         1257 c         1270 g          733 t
ORIGIN      
        1 tcgctcgcgc ccctccgatc ggttccggat acccctgacg cggcacggag ctcgtccgcc
       61 agcgcctcca gcctttccag ccgccgggcc gccaggacga gccgggcccc ctcgcgatgg
      121 aacgcccgcg cggtggccgc gccgatgccg gacgacgcgc ccgtgatgaa cgccagtcgt
      181 ccggccagga gcccggcgcc gcccgccggc cgcgaatcgg tcacggcgcg gtcggatctt
      241 tcagttcgat ggtcgtcacc gggccgatcg acgagagcgg cttgtcgaag ttggccgccg
      301 cgccgaccac caggatctgg agctggtcgt ccggcaggta cttcctggcc atggcctgca
      361 cgtcctccgc gctcaccttc gcgagcgcct ccaggcgctt cgtcggcaaa tcctgcggca
      421 ggccgtagta agccaggtcg aggacgctgg ccagcacctt gtccggctgg tcatagttga
      481 agacctcggc gttcaagagg ccgttggtgg cccgggagag ctcggccttc gtcaccggct
      541 cgtcgcgcat gcggccgatc tcgcgcagca tgatctcgag ggccttgacg gtcgattccc
      601 ccttcgagat ggcgtacacg atctccgggc ccggatcgag attgccgagc tggagggcgg
      661 agcccgaggc gtacgccagc ccctcgtcgc tgcgcaccgc ctcgacgacg cggctggtga
      721 agccgcccga gccgaggatg aagtccatca cctcgagcgc cgccgcgtcc ggatcaccga
      781 ctttcgtgcc gagccggctg atcaggatgg tgctctgggt ggcgctcgtc ttgtcggcct
      841 ggaaaatggc ccggggggtg ttcatcggca ccggcggcac cgacggcagc tcgaccgttg
      901 cgtcgggcca gcttcccatg gccgcctcga tcgtggccgc gatcgtcttc gggtcgaagt
      961 cgccggccac gccgatcagg aagttcgacg gcacgtaata cttcttgtgg aagtccacca
     1021 ggtcctggcg ggtgatggca tcgaccgtgg cgaaggtggg cgcgaatccg cgcgggtggt
     1081 cggtgccgta gagccggcgc ggcagttccc gcagcgcgat ctgcaccggg ttatcgttct
     1141 ggcggcggat gccgtccttg atcttctcct tggccagcgc cagcttctcg gcgtcgaaag
     1201 cgggctcgcg cagcatctcg aacaggaggt tcagggccgg ctggaagttc tcggtgagca
     1261 cctggaacga ggcgttgccg ctctcgttcc ccacgcccgt cgacacgctc atcgccagcc
     1321 ggtccacttc cgcatccacc tggtcccacg ggcgcttggc ggtgccgccg gtcctgagga
     1381 ggttccccat caggccggca gtgcccgcct tccccggcgg gtcgtagagc gcccccgtcc
     1441 gcacggtgcc gtagaaactg atcagcggaa ggcggtgatc ctccatgagg tacacggtga
     1501 cgccattcct gagcttgatg gtggtgggct cgggcttctt ccactcgagc ggcgtgatct
     1561 tgacgtcctt ccaggacctg gcaggcgtgg cggccgttgc cggcgcgacc gggttcgcgg
     1621 caaagagtcc cgcggccatt accagcatgg ccccggcccg gaagcgccgc gtcgtttcgt
     1681 tgctccgcat catggtgagc tccttcatga gggcttccgg aactcggcgg gggtgacgcc
     1741 cgagttgacg gtccgcttcg tcgtcagttt ctcgaggatc ttctccccgc ccatccagat
     1801 ctcttccttg gccggccacc agacgcccga cacttcctgc cagtcgagca gcgcctcctc
     1861 ggcgcggccg ggctggccgg tcaggggatt ctccgtatcg aacgaccgcc cgcgtgggcg
     1921 attcgtggcc ggatcaaggt agaggttgaa gggcctcccc tccaccggcg tgatctcgat
     1981 gatgatcgag ggcttcccct ggaagtcgcc atccgggagc cgccgggccg tgccgttcgg
     2041 gttctggccg acgtgcttca ggagatacac ctcgcgggtg aacccttcct tcatgtcgga
     2101 gagcgccgcg gcgtcggcat cctgcattcc gcccgggctg gtgatccagc ccgtctcgcc
     2161 gtccagtgtc tgtacctgcg cctgacccat gatcgagagt tcggtcttca tccgcccgtc
     2221 gatcgtgaac gtctgtttct gggaagccgg aatcgcctgg ccctgcgcaa agatcgtgag
     2281 agtggattca acggtcaggt ccttgagacc ggccagcgtg gcctccttcc cgaatgacga
     2341 gcgggccttg ccgaggattt ccatcgcctc ggcctcggct ttcggatcga cgaacgcggc
     2401 ctcgggcgtc ggcttcacga gagtgccgac tgtccggttg gacgcgttga agtaggtcga
     2461 tgccacccgc tggatatcgg ccggcgtcac gttcgcaacg gcctcggccc gacggaaact
     2521 gtcgcgccag tcgcccagcg tggcgttgtt gtacgccagc gcggtggcca ggcccagatt
     2581 gtcctcgagc ccgttcacga attgcttgcg atagcggagc ttggcggcgg ccagttcggc
     2641 ttccgttggc ggctccttct tgaggcgggt gatctcggcg tacatggccg cctccacttc
     2701 ctgcgtggta tgcggcggga gtggcgtcgc ctcaaggatg aagaggccgg ggtagcgctc
     2761 acccgggatg ccggtgtagc tggcgatgct cgaggcgatc ttctgctttt cgaccacgtt
     2821 gttgatcatt cggctcgatc ggccgccggt gaggatgtct tccatcatca ggagcgccgg
     2881 atagtcggga tgatgcccgt cggtgatgtg ccaggcggcc agcacccgcg tctcggcgtc
     2941 gtactccacc aggaaacggc gctcgccctt ctgcatcggc tccacggtac gtggaggaat
     3001 cgggtcgggc tgccgaggga tggaaccgaa gtaggccttg gccatttcgg tcaccttgtc
     3061 ggggtcgatg tcacccacga tccccagcgt gacgcggttc ggcgcgtaat acttcttgaa
     3121 gaacgcctcg acctcggtgc gcgtgacctg ctggatgtcg ctcggccagc cgatcaccgg
     3181 cacgccataa cggtgcgcgg tgtaggcggt ggcgtagatg gcctcaccca cctttcccag
     3241 ggggccggcc ggctggtcgt cggtgcggag gcgcctctct tccatgatgt tgccgagctc
     3301 ggtgtagaac tcccgaagga cgggcgcctt gagccggtcg ctctcgattt tcatccagag
     3361 ctcgaggcgg ttcgacggga gggcaatctc gtagttggtg ccgtcccagg acgttccggc
     3421 attgaacccc tggctgccgt tctccgtgta gacccggtcg tatgggttgg gctccacgta
     3481 gccgcgggct gccttctgaa gcgaatcgag ctccgtctgg agcatgagga gtcgcccgtt
     3541 gtcggcccgg ctttccttgc cccgttcgag gcgcaggtcc tgggcggtcg tctcgatccg
     3601 gtcgaggagg agcttctcct tcttccagtc ggtcgaaccc atgtcggccg tccccttgaa
     3661 gaggagatgc tccaggatgt gggcgctacc cgagatgccg tcccactcgt cgaccgatcc
     3721 gaccgcgaac tgcacgatca ggccgatcgt cggcgactgg tgattctcga ccatcaggat
     3781 ggtcatgccg ttg
//
LOCUS       DASVOD010000115         1971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_8460, whole genome shotgun sequence.
ACCESSION   DASVOD010000115 DASVOD010000000
VERSION     DASVOD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8460"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VF720_02220"
     CDS             complement(<1..655)
                     /locus_tag="VF720_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878193.1"
                     /translation="MLHGLAILGIGAALPVSWAHADLKQAQSLQAAGKIEAAREAYEE
                     VLTHGDNREAMYQLALLSQSGHDYVRYLQQFLEAGGRRDKRAAEVEVRLGRYHYTAGD
                     YHAALDQLESARERRTDPETRVEVRFWLGQTLLALRERDDARRAFESVISDRAGGPWK
                     SRALYSLGEILRVSGDPGAAARTFAQLRSDSDLGAAAMLAEAQCREKGGDRREAAALY
                     "
     gene            complement(697..>1971)
                     /gene="miaB"
                     /locus_tag="VF720_02225"
     CDS             complement(697..>1971)
                     /gene="miaB"
                     /locus_tag="VF720_02225"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX7878194.1"
                     /translation="AVLAGRFKLEGCHVVATPEEADIILVNTCAVRENAEERVFGRIG
                     ELKRVTSANGALLGVTGCMAQRLGGEILERAKHVDLVVGTGAYGKVFELLEARRETGQ
                     PQIDLSFGQRFTEIERPRLAPGEIKTFLSIMEGCNKSCSYCIVPYTRGKERYKSLGMI
                     RREAEELVARGVKEITLLGQNINSWRDGKWRFADVLTEVNRTPGLERIRFTTSHPINT
                     DEKMLEAMAAGDKVCETLQLPIQSGSDRILALMRRGYTRASYDRLIARGRELMPNLAV
                     TTDLIVGFPGETNDDFAATVRAMDEIRFDSAFMFAYSERRGTRACAMPDPVPAEVAAE
                     RLQTIIALQRRHTEAIGRSRIGSTEEVLIEALAPRRPDHVIGKSRTFKPVVMPGPLEW
                     IGTTRTVSIRSTQGVTLFGEALPIGTPAAVGS"
BASE COUNT          292 a          704 c          648 g          327 t
ORIGIN      
        1 cgtagagggc tgccgcctcg cgccgatctc cccccttctc gcggcactgg gcttcggcca
       61 gcatcgccgc ggcgccaagg tccgaatcgg agcggagctg ggcgaatgtc ctggccgcag
      121 cgcccggatc gccggagacg cgcagaatct cccccagaga atagagcgcc cgacttttcc
      181 aggggccgcc ggcccgatcg ctgatgaccg actcgaaggc ccgccgggca tcatcgcgct
      241 cgcgcagggc caggagcgtc tgccccagcc agaatcgcac ttccacgcgg gtctcgggat
      301 cggtacgtcg ttcgcgggcc gactccagct ggtccagcgc cgcatggtag tcgcccgccg
      361 tgtagtggta ccggccgagg cgcacctcca cctcggcggc ccgcttgtcc cgccggccgc
      421 cggcctcgag gaactgctgc aggtagcgca cgtagtcgtg ccccgactgc gagaggagcg
      481 cgagctgata catggcctcg cggttgtcgc catgcgtgag aacttcttcg taggcctcgc
      541 gggcggcttc gatctttccg gcggcctgga gggactgcgc ctgtttcagg tcggcatgcg
      601 cccacgatac cgggagtgcg gcgccgatcc ccaagatcgc caaaccgtga agcatgagcg
      661 taccgacgcg cccgaccgtc cgagtcgtcg gccggatcat gaaccgacgg cggcgggcgt
      721 cccgatcggc agggcctcgc cgaagagcgt cacgccctgc gtggagcgga tcgacaccgt
      781 gcgcgtcgtg ccgatccact cgaggggccc cggcatcacg accggcttga aggtacgact
      841 cttgccgatc acgtggtcgg gccggcgcgg cgccagggcc tcgatcagca cctcctcggt
      901 gcttccgatc cgcgaacgcc cgattgcctc ggtatgccgc cgctgcaggg cgatgatcgt
      961 ctggagccgc tcggcggcca cttcggccgg caccgggtcg ggcatggcgc aggcccgtgt
     1021 gccgcggcgc tcggagtagg cgaacatgaa ggccgagtcg aaccggatct catccatggc
     1081 gcgcacggtg gcggcgaagt cgtcgttcgt ctcccccggg aaaccgacga tgaggtcggt
     1141 cgtgacggcc aggttcggca tcagttcgcg accccgggcg atgagcctgt cgtagctcgc
     1201 gcgcgtgtag ccgcgcctca tgagcgccag gatgcgatcc gagcccgact ggatcggcag
     1261 ctgcagcgtt tcgcagacct tgtccccggc cgccatcgcc tcgagcatct tttcgtcggt
     1321 attgatcgga tgactggtcg tgaaacggat gcgctcgagg ccgggcgtcc tgttgacctc
     1381 ggtgaggaca tcggcaaaac gccacttgcc gtcgcgccac gagttgatgt tctgtccgag
     1441 cagcgtgatc tccttcacgc cgcgcgccac cagctcttcg gcctcgcgcc ggatcatccc
     1501 gagcgacttg tagcgctcct tgccgcgtgt gtacggcacg atgcagtacg agcagctctt
     1561 gttgcacccc tccatgatgg agagaaaggt cttgatctct cccggggcca ggcgcggtcg
     1621 ctcgatctcg gtgaaacgct ggccgaacga caggtcgatc tgcggctgcc ccgtctcccg
     1681 gcgcgcctcg aggagctcga acaccttgcc gtaggcgccg gtgccgacca ccaggtcgac
     1741 atgcttcgcc cgctcgagga tctcgccccc cagccgctgt gccatgcacc ccgtgacgcc
     1801 caggagcgcc ccgttcgccg acgtgacccg cttcagctcg ccgatccgcc cgaacacccg
     1861 ttcctcggcg ttctcccgca cggcgcaggt gttcaccagg atgatgtcgg cctcttcggg
     1921 ggtggccacc acgtggcacc cctccagctt gaaacggccc gccagaaccg c
//
LOCUS       DASVOD010000116         2786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_686208, whole genome shotgun sequence.
ACCESSION   DASVOD010000116 DASVOD010000000
VERSION     DASVOD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686208"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..763)
                     /locus_tag="VF720_02230"
     CDS             complement(161..763)
                     /locus_tag="VF720_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7878195.1"
                     /translation="MRSTTPARYRVPTPEEEAVLVRACLAGDAAAWKKLLARYETVIW
                     MIPRRFGLSEEDRAEVFQSVCLALYRGLPRLRQAAGLTRWVLTTAHRQSRDLARKRRR
                     EAPLPVEGDMADIPSEDPSPARLHEEASLRVAIRAAVDRLPPRCAELLQLLYLEAVPL
                     SYREVAKRFDVPEGTIGPTRARCLSRLREILEKDLRGGTK"
     gene            842..1105
                     /locus_tag="VF720_02235"
     CDS             842..1105
                     /locus_tag="VF720_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878196.1"
                     /translation="MDERLEAKLHDASARAERLGDDLKSKGRELLDKVDASARDFKAK
                     HIDDIADDVGNFVKKHPGTLILASFVVGVALGALLRGNGRRED"
     gene            complement(1118..1444)
                     /locus_tag="VF720_02240"
     CDS             complement(1118..1444)
                     /locus_tag="VF720_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878197.1"
                     /translation="MTPTKPAGKASVAELQARVAAAERDLHASIDDIGLVVEDALDWK
                     KKVREHPVESAALAALVGFLAMRQPGIVPGLVRQVGKVGLASLAQAGGGPLLGSLLAR
                     FTGRNE"
     gene            complement(1441..1797)
                     /locus_tag="VF720_02245"
     CDS             complement(1441..1797)
                     /locus_tag="VF720_02245"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX7878198.1"
                     /translation="MNTPNEEPPRPGLSDLPRLIRSTVDRGIDLASARIELIQAEIRE
                     EAEKRLRRLPHIVIPAVFLVLGFMLLNVALVAWLAEAIGLTAAAFSLAGLYLAIGAIG
                     TWLFLKGGGLNPPDRP"
     gene            2042..>2786
                     /locus_tag="VF720_02250"
     CDS             2042..>2786
                     /locus_tag="VF720_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013028262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7878199.1"
                     /translation="MADNENSLAVFVDFENLALGFEGRRGPRFEIQKVLERLVEKGKI
                     IVKRAYADWGRFREYRSGLHEAAIELIEIPRRSMTGKNSADIRLVVDAMDLSYSKAHV
                     DTFVIISGDSDFSPLVSKLKENGKQVIGIGMKSSTSDLLVDNCDEFIFYEDLDRPAIS
                     VNTAEMGSVPTEKKKLFSLVMEALAALRRENKEVIWSSMVKDTIKRKRPQFNETYYGY
                     RSFSNVLEDAQRFGLVSLTTDPRSGTYVVS"
BASE COUNT          500 a          924 c          906 g          456 t
ORIGIN      
        1 gctgctcgac aaagtcgagc agcgcggctt ccgtgacatg tcccgaatgg aagtggggac
       61 ccagcatgct caccagattt ctccctgcgg tgacatcacc gcaaccccgc gaccgtgacg
      121 ggaaatttga cgtttacatt cctaatgtgg cgcatcggcc tcatttcgta ccacctctta
      181 aatctttttc cagtatctcc cggagacgtg agaggcaacg cgcccgcgtg gggccgatcg
      241 ttccctccgg cacgtcgaac cgcttcgcca cttcccgata ggagagcgga acagcttcaa
      301 gataaaggag ttgcagtagt tccgcacagc gtggggggag gcggtccacc gcggcccgga
      361 tggccaccct cagcgaggcc tcctcgtgga gccgggccgg ggaggggtcc tcgctcggga
      421 tgtcggccat atctccttcg acggggagtg gcgcctcccg gcggcgcttc cgggccaggt
      481 ccctcgactg ccgatgggcg gtggtgagca cccaccgggt gagccccgcc gcctgccgga
      541 ggcggggcaa cccccggtac agggccaggc acaccgactg gaagacctcg gccctgtcct
      601 cctcggagag accgaagcgc cgcggaatca tccagatcac cgtttcatag cgggccagga
      661 gttttttcca ggccgcggca tcgccggcca ggcaggcgcg gacgagcacc gcctcctcct
      721 ccggggtcgg cacgcggtag cgggccgggg tcgtcgaacg caatggtggt cgcggcaaat
      781 tcggttagac tcccatctcg ttcgacccac cggaattcgt ccgaagagaa aggatcacgt
      841 catggatgaa agactcgaag ccaagcttca cgacgcctcc gcccgcgccg agcgcctggg
      901 cgatgatctg aagtcgaagg gccgggaact gctcgacaag gtggacgcca gcgcccgcga
      961 tttcaaggcg aagcatatcg atgacatcgc cgacgacgtg ggcaatttcg tgaagaagca
     1021 ccccggcacg ctgatcctgg cgtcgttcgt ggtgggtgtg gccctgggag cgttgctccg
     1081 ggggaacggc cgtcgcgaag actgacggcc cggaacgcta ttcgttgcga ccggtgaacc
     1141 gggcgaggag gctgcccaga agcgggccgc ctcccgcctg tgccagcgac gcgagcccca
     1201 cctttcccac ctggcgcacc agccccggca cgatccccgg ctgacgcatc gccaggaaac
     1261 ccaccagggc cgccagcgcg gccgattcca ccgggtgctc gcgtaccttc ttcttccagt
     1321 cgagggcgtc ttccacgacc aggccgatgt cgtcgatgga ggcgtgcagg tcccgctcgg
     1381 ccgcggccac gcgcgcctgc agttcggcca cgctggcctt gcccgccggt ttcgtcggcg
     1441 tcatgggcgg tcgggcgggt tgagcccgcc ccccttgagg aagagccagg tgccgatcgc
     1501 gccgatggcc aggtagaggc cggccaagct gaaggcggcg gccgtcaatc cgatcgcctc
     1561 cgcgagccag gccaccagcg ccacgttcag cagcatgaag ccgagtacga gaaagactgc
     1621 ggggatgacg atgtgaggga gccgccggag gcgcttctcc gcttcctcgc ggatctcggc
     1681 ctggatgagc tcgatgcggg ccgatgcgag gtcgatgccg cgatccacgg tgcttcggat
     1741 gagtcgcggc aggtcggaga gtccggggcg gggcggttcc tcgttcgggg tgttcatggg
     1801 cgcattgtgg acaatccgaa cgcctccgcc aagtcggtgg gcgacaggcg gcccgggatg
     1861 gcgtatgatg ccggcgctgc gattgcctta cccgccacgc ggggcgcttg gaatgcgcgg
     1921 caggtcgccg gatccctccg gccaccaatg acggcccgct ctccccacgg acctttgccg
     1981 tatcgggatg gcgcgcccgg gtgggcgccg attgccccgc ccgttgctaa ctggagaaaa
     2041 catggctgac aacgaaaact ccctcgccgt attcgtcgat ttcgaaaacc tcgccctcgg
     2101 attcgagggg cgccgcggcc cgcggttcga aatccagaaa gtcctcgaac ggctggtgga
     2161 gaaaggaaag atcatcgtca agcgcgccta cgccgactgg ggccgctttc gcgaataccg
     2221 ctccggcctg cacgaggccg ccatcgaact gatcgagatc ccccgccggt cgatgaccgg
     2281 aaagaattcc gccgacatcc gcctcgtggt cgacgcgatg gacctctcgt actcgaaggc
     2341 ccacgtcgac accttcgtca tcatctcggg cgattccgac ttctcgccgc tcgtctccaa
     2401 gctgaaggag aacggcaagc aggtcatcgg catcgggatg aagagctcga cgtccgacct
     2461 cctggtcgac aactgcgacg agttcatctt ctacgaagac ctcgaccgcc cggccatctc
     2521 cgtcaacacg gccgagatgg ggagcgttcc gacggaaaag aagaagctct tctcgctggt
     2581 gatggaggcc ctggccgccc tgcgccgcga gaacaaggaa gtgatctggt cgtcgatggt
     2641 gaaggacacc atcaagcgga agcgcccgca gttcaacgaa acctactacg gctaccgctc
     2701 cttctccaac gtcctcgaag atgcgcagcg cttcggactc gtgtcactga ccaccgatcc
     2761 ccgcagcggg acgtacgtgg tgagcg
//
LOCUS       DASVOD010000117         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_425624, whole genome shotgun sequence.
ACCESSION   DASVOD010000117 DASVOD010000000
VERSION     DASVOD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425624"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..605
                     /locus_tag="VF720_02255"
     CDS             141..605
                     /locus_tag="VF720_02255"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX7878200.1"
                     /translation="MSMPKPGDAHAKLARLAGKWRGTETIHPSPFDPAGGQAEGRVDN
                     RIVLDGFVVVQDYEQERGGAVNFRGHGVFSFDPERNVNVMTWWDSFGGTRSEYLGNWS
                     GDVLTLEASTPQGPARAVFDLATAGQYGFRMEVSPDGNQWFPFMEGRYRRID"
     gene            complement(613..1014)
                     /locus_tag="VF720_02260"
     CDS             complement(613..1014)
                     /locus_tag="VF720_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878201.1"
                     /translation="MSTPDKPEQGAITWIDLTVPNADDVRRFYADVVGWATERVSMGE
                     YDDFNMVGPKSGRAMAGVCHAQGMNADLPAQWLIYINVANLDHSIARCQELGGTIVRP
                     ATPMGEYGRYAVIRDPAGAVAAIFEPASGVG"
     gene            complement(1027..1479)
                     /locus_tag="VF720_02265"
     CDS             complement(1027..1479)
                     /locus_tag="VF720_02265"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7878202.1"
                     /translation="MTTQSTNPKTFLSQPEAARVVDAIKAAEKGTTGEIRVRIARKCG
                     RPPLEAAQITFHALRMDQTAQRNGVLLYLSLVDREFAVFGDEGIDRHIGCDGWNAIRD
                     RLADRFKKDQFADGLAEAITDIGKTLAKHFPGKAGDRNELSDDLSLGR"
     gene            complement(1484..2305)
                     /locus_tag="VF720_02270"
     CDS             complement(1484..2305)
                     /locus_tag="VF720_02270"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEX7878203.1"
                     /translation="MIPVRFLAALLLLAAVLALRPVSAAAEFVLPPPPGRHIVDKAGI
                     LDPGSISYLEERLTRFEQETGHQMVIAIFKGIEGDDLEEVSNKLFRKWGLGDKTANDG
                     ILLAIFYQERISRVEVGYGLEGVLTDARCARILRNEFQPEFQTGRYGQGLRRAVVAIE
                     QLVRDPSSAPPDTTAREKKKSSSSFWIAILAFFIIFAFSMASARSRRGGSELTGSGRR
                     RYRDPWDDGWGGGGFGGFGGFGGGGGGWGGGFSGGGGGFSGGGGSSGGGGASGKW"
     gene            complement(2361..>2691)
                     /locus_tag="VF720_02275"
     CDS             complement(2361..>2691)
                     /locus_tag="VF720_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7878204.1"
                     /translation="PEMLKDPQALKNFEAAQGQLTSALSRLLAVAENYPQLKANQNFL
                     DLQSQLEGTENRIAVERRTFNQVAQTYNTKIRIFPTSLLAGMFGFTPRSYFEAAPGSE
                     KAPTVKF"
BASE COUNT          440 a          879 c          920 g          452 t
ORIGIN      
        1 attggggggg gctgcatact cggcggcgcg ccgcggcgcc gttcacgccc gacgacgaag
       61 aacctggatg aaaaactgat tccacatgcg cgccctccgg gcgcgcatag cccttcgaca
      121 ttttttttga aggaggcacc atgtccatgc cgaaaccggg cgacgcccac gcgaaactcg
      181 cacgactggc cggcaagtgg cgcggcaccg aaacgatcca cccctcgccg ttcgacccgg
      241 ccggtggcca ggccgaaggg cgcgtggaca atcggatcgt gctcgacgga ttcgtcgtcg
      301 tgcaggacta cgagcaggag cgcggcggcg cggtcaattt ccgcgggcac ggcgtcttca
      361 gcttcgatcc ggagcgcaac gtgaacgtga tgacctggtg ggattcattc ggcggcacgc
      421 ggagcgagta cctggggaac tggtcgggcg atgtcctcac gcttgaggcc agcacaccgc
      481 agggaccggc ccgcgccgtg ttcgatctcg cgacggccgg ccaatacggg ttccgcatgg
      541 aagtgagccc ggacgggaac cagtggtttc ccttcatgga ggggcgctac cggcggatcg
      601 actgaagcct gatcagccta cgccgctcgc cggctcgaag atcgcggcca ccgcaccggc
      661 cgggtcgcgg atcaccgcgt agcggccgta ctcgcccatg ggggtggcgg ggcgcacgat
      721 cgtgccgccc agctcctggc agcgggcgat cgagtgatcg aggttcgcca cattgatgta
      781 gatgagccac tgggccggca ggtcggcgtt catcccctgc gcgtggcaca cgccggccat
      841 ggcccgcccg ctcttcggcc ccaccatgtt gaagtcgtcg tactccccca tgctcacgcg
      901 ttcggtggcc caaccgacca cgtcggcgta gaagcgtcgc acgtcgtcgg cattggggac
      961 ggtcaggtcg atccaggtga tggcgccctg ttcgggcttg tcgggcgtgg acacgcgggc
     1021 ctcccgtcag cgccccaacg acaggtcgtc cgaaagctcg ttccggtcgc cggccttgcc
     1081 ggggaagtgc tttgccagcg tctttccgat gtcggtgatc gcttcggcca ggccgtcggc
     1141 gaactggtcc ttcttgaaac ggtccgccag gcggtcgcgg atggcgttcc agccgtcgca
     1201 gccgatgtgg cgatcgatcc cttcgtcgcc gaagacggcg aattcccggt cgaccagcga
     1261 gagatagagg agcacgccgt tccgctgggc agtctggtcc atcctgaggg catggaaggt
     1321 gatctgcgcc gcctcgagcg gcgggcggcc gcacttccgg gcgatacgca cgcggatttc
     1381 gcccgtcgtc cccttctccg cagccttgat cgcgtcaacg acgcgcgccg cttcgggctg
     1441 ggagaggaac gtcttcgggt tggtgctttg tgtcgtcatg cgctcaccac tttccactgg
     1501 cgccgccgcc gcccgacgaa ccaccgccgc cggagaagcc gccgccaccg cccgagaaac
     1561 cgccgcccca acccccgccg ccgccgccga aaccgccgaa acctccgaag ccaccgccac
     1621 cccagccgtc gtcccacgga tcacggtagc ggcgtcgccc ggagccggtc agttcggatc
     1681 cgccgcgcct cgaccgggcg gaggccatcg agaaggcgaa gatgatgaag aaagccagga
     1741 tggcgatcca gaagctcgag gatgacttct tcttctccct tgccgtggtg tcgggcggcg
     1801 ccgacgaggg gtcgcgcacg agctgctcga tcgccaccac cgcacgccgg agtccctggc
     1861 cgtagcggcc ggtctggaac tcgggctgga attcatttcg gaggatgcgg gcgcagcggg
     1921 cgtcggtgag aacaccctcg aggccgtagc ccacttccac tcgcgagatc cgctcctggt
     1981 agaagatggc cagcaggatg ccgtcgttgg cggtcttgtc accgagtccc cacttgcgga
     2041 acagcttgtt gctcacctcc tcgaggtcgt ccccttcgat ccccttgaag atggcgatga
     2101 ccatctggtg gccggtttcc tgctcgaagc gggtaaggcg ttcctcgagg tacgagatcg
     2161 agccggggtc gaggatcccc gccttgtcaa cgatgtggcg gccggggggc ggcggcagga
     2221 caaactcggc cgcggccgac acgggacgga gtgcgaggac ggcagccagc agcagcaacg
     2281 cggccaggaa gcgtacgggg atcatgggcg agatcgatcg gaatgagcgc ggcatccgtg
     2341 tcacggggat ggtcaagcgg tcagaacttc accgtgggcg ccttctcgga gcccggtgcg
     2401 gcctcgaagt acgagcgcgg tgtgaaaccg aacatgccgg ccaggagcga cgtgggaaag
     2461 atgcggatct tcgtattgta ggtctgcgcc acctggttga acgtgcggcg ctccaccgcg
     2521 atccggtttt ccgttccctc gagctggctt tgcaggtcga ggaagttctg gttggccttg
     2581 agctgcggat agttttcggc gacggcgaga agtcgggaga gtgccgacgt gagctgcccc
     2641 tgggccgcct cgaaattctt gagggcctga gggtccttca gcatctcggg c
//
LOCUS       DASVOD010000118         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_790701, whole genome shotgun sequence.
ACCESSION   DASVOD010000118 DASVOD010000000
VERSION     DASVOD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790701"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VF720_02280"
     CDS             complement(<1..292)
                     /locus_tag="VF720_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7878205.1"
                     /translation="MMRLPKFTYRRPTSVKEAVALAAGEGHNAMFVAGGTDLYPNMKR
                     RQQTPATVIGLSRIEELRAISGAPMTGMTIGAGLTLTEVESNPVLAEAYPGLV"
     gene            complement(289..564)
                     /locus_tag="VF720_02285"
     CDS             complement(289..564)
                     /locus_tag="VF720_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878206.1"
                     /translation="MMTEPVKVVGAVFQLVGLTLMGYAYFRHRENEQFPVRSVWALVR
                     ATPEQKRRLAAWYQTPTFRIHLTGWLLFGIGLVLFYLSEPIQGIFAP"
     gene            complement(561..>2133)
                     /locus_tag="VF720_02290"
     CDS             complement(561..>2133)
                     /locus_tag="VF720_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7878207.1"
                     /translation="GGGYGSYGVASTFYTGALQTVTYQVPKYKFEGARFFTNKPPCGP
                     KRGHGTPQPRFALEVQLDKIAEALNLDPAEMRKQNLHPPNTLTANYLRVGSMGLGKCI
                     DKVVEGSDWKNRYRKLPYGRGLGLACSSYITGAGLPIYWNAMPHSGVQLKCDRGGGVT
                     VFCGSTEIGQGSDTILAHITAEILGVDPFDIRVVTADTDLTPVDLGSYSSRVTIMTGN
                     ACIQAAERARELLAKAAAEKLSKPKKPKPGQPALPPPPQVPASRLVFAERRVFDSENP
                     EIGMSFAEAVVAAETKFGTIGTVGSYVPPPSAGQYKGAGVGPSPAYSYSAAVVEVDVD
                     PETGIITVPKIWVGHDIGQCINPILTMGQVEGGVYMGLGEALMEEMTYRENRNVVHKI
                     PSLLEYKSPTTMEMPDVITYLIEDPDPNGPYGAKEVGQGPLLPIMPAVANAVYDAVGV
                     RIDEIPVTPDKVYKALREKAAGRNPRVGPKKMPHLDWGEPIRMKTPAEGGDGHPISEP
                     EPRKKKVEPAAGVRS"
BASE COUNT          322 a          687 c          727 g          397 t
ORIGIN      
        1 cgacgagccc gggatacgcc tctgccagca ccgggttcga ttcgacttcg gtcagcgtga
       61 gccccgcgcc gatcgtcatt cccgtcatcg gcgcgccgga gatggcgcgc aattcctcga
      121 tgcgcgagag gccgatcacc gtggcgggcg tctgctgacg gcgtttcatg ttcggataga
      181 gatccgtgcc gccagccacg aacatggcat tgtggccctc gcctgcggcg agggcgaccg
      241 cttccttcac cgatgtgggg cggcggtagg tgaatttcgg gaggcgcatc atggcgcgaa
      301 gatcccctgg atcggctccg agaggtagaa gaggacgagt ccaattccga acaggagcca
      361 gccggtgagg tggatccgga atgtcggcgt ctggtaccag gcggcaagcc ggcgcttctg
      421 ctcgggggtg gcacggacca atgcccacac tgaacgaacc gggaattgct cgttttcccg
      481 gtgtcggaaa taggcgtagc ccatcagggt gagtccgacg agctgaaaga cggctcccac
      541 gaccttcacc ggctccgtca tcacgaacgc actcccgccg ccggctccac cttcttcttg
      601 cgcggctccg gctcactgat cggatgccca tcgcccccct cggccggcgt cttcatccgg
      661 atcggctcgc cccagtcgag gtgtggcatc ttcttcggtc ccacgcgcgg gttccgaccg
      721 gccgccttct cacgcagtgc cttgtacacc ttgtcgggcg tcacggggat ttcgtcgatg
      781 cggacgccga cggcgtcgta caccgcattg gccaccgcgg gcatgatcgg gagcaacgga
      841 ccctggccca cttccttggc gccgtacggg ccgttgggat cggggtcctc gatgaggtag
      901 gtgatgacgt cgggcatctc catcgtggtc gggctcttgt actcgaggag gctggggatc
      961 ttgtgcacga cgttccggtt ttcgcggtac gtcatctcct ccatcagggc ctcgccgagg
     1021 cccatgtaca cgccgccttc cacctggccc atcgtgagga tggggttgat gcattggccg
     1081 atatcgtggc cgacccagat cttcggcacg gtgatgatgc cggtttcggg atcgacatcc
     1141 acttccacca ccgcagcgct gtacgaatac gcgggcgacg ggccgacgcc ggcacccttg
     1201 tactggccgg ccgacggcgg cggcacgtag ctccccacgg tgccgatcgt gccgaacttg
     1261 gtttccgcgg ccacgacggc ttcggcgaac gacattccga tttcgggatt ctcgctgtcg
     1321 aagacgcggc gttcggcgaa aaccaggcgt gacgccggaa cctggggcgg cggcggaagc
     1381 gcgggctggc cgggcttcgg cttcttcggc ttcgacaatt tctcggccgc cgcctttgcc
     1441 agcagctcgc gcgcgcgctc ggccgcctgg atgcaggcgt tgccggtcat gatcgtgacg
     1501 cggctggagt acgagccgag gtccaccggc gtgaggtcgg tgtcggccgt cacgacgcgg
     1561 atgtcgaacg gatccacacc gaggatttcc gccgtgatgt gcgccaggat cgtgtccgat
     1621 ccctggccga tctcggtgga gccgcagaag accgtgacgc caccgccgcg atcgcacttg
     1681 agctgaaccc cggagtgcgg catggcattc cagtagatcg gcagaccggc gccggtgatg
     1741 tacgagctgc aggcgaggcc gagccccctc ccgtagggga gcttgcggta gcggttcttc
     1801 cagtccgaac cttccacgac cttgtcgatg cacttgccga gccccatcga gcccacgcgg
     1861 aggtagttgg ccgtgagcgt gttggggggg tggaggttct gcttgcgcat ctcggccggg
     1921 tcgaggttga gtgcctcggc gatcttgtcg agctgcacct cgagcgcgaa gcggggctgc
     1981 ggcgtgccgt ggccgcgctt ggggccgcac ggcggcttgt tggtgaagaa acgggcccct
     2041 tcgaacttgt acttcggcac ctggtaggtg acggtctgca gcgcgcccgt gtagaacgtg
     2101 gaggccacgc cgtacgagcc gtagccgccg ccg
//
LOCUS       DASVOD010000119         6703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_373249, whole genome shotgun sequence.
ACCESSION   DASVOD010000119 DASVOD010000000
VERSION     DASVOD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6703
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373249"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF720_02295"
     CDS             <1..184
                     /locus_tag="VF720_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878208.1"
                     /translation="YGNYLANSAVSHLYFKDGRMEMFEFIDTKTQESFQVTNPSDRLA
                     LQSRPPETKTGGGGSH"
     gene            247..1014
                     /locus_tag="VF720_02300"
     CDS             247..1014
                     /locus_tag="VF720_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878209.1"
                     /translation="MPPSSDMRRPGKPAGPLAAFVLATLVAGSSLIAELVRPTAAAAG
                     GNPNAKIVLHLVPWNNRHTCAAGELAGPGQVVTKGDLFPARYAAYALIVDGNRGEGIS
                     GVQFGIAFNDTLKKGVDIIDWQECSLFNWPMPGWPVETATGNLLTWNVVTDCDTTGFR
                     VAGFFYLTAYSPDRLRIIPRPADNMAAVIDCLKNGSKNEDCLDVIPQENLGFVDFGGG
                     DGYNPWDPKQNLMKFKNPGGVRIREGTTPPDKKPGGR"
     gene            1021..1749
                     /locus_tag="VF720_02305"
     CDS             1021..1749
                     /locus_tag="VF720_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEX7878210.1"
                     /translation="MAAAQLPVYSLLLFAAAAFGGLLPTLRPELTKKSRAFILSLASG
                     LLLGTAFLHMLPEAVNIVGASVVVAVLVGFVSLLLLEKFVMVHPCEEMGCHFHHLGTS
                     AFLGISLHSFMDGVALGASLSDAGLSLVVFGAILLHEIPCGFSLGSLLVLGRRDRGRV
                     IALILLYAIMTPLGAILTWLLSLQLPPTTAGWALGFSAGNFLFVATADLLPQLHLHER
                     ANRRQIVWLLAGLGISWLGLLLEG"
     gene            1852..2817
                     /locus_tag="VF720_02310"
     CDS             1852..2817
                     /locus_tag="VF720_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878211.1"
                     /translation="MLAWRQRKTQFAALLGFLALLPLTAAPAGAQATNWTGTLGNWSL
                     GSNWSAGEPNATVDAVFNQLGTATVNMTNESCRNLLIGNDGVSASVTVQAPGTLAVVG
                     SIRVPYQLSGAFNHQSGLVTCDSLLIGNVPLKIGQYLMQGGNLTVGTVQMGMVQNSAA
                     TFSIGGLNTHVSVGHSLYVGRGGNLVSGGGTFTVGSMNTDSLVVIGSFQMVNQPTVNV
                     TNFVLRNLSGITYTFLPTGITPVISSGALILDGTLTVFDAGAPNGTYEILRGNSISGT
                     FDTVTLPLVGDWSWRIDGNSVFVSKGIVAVEPTTWSDVKTRSVRN"
     gene            2944..3801
                     /locus_tag="VF720_02315"
     CDS             2944..3801
                     /locus_tag="VF720_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878212.1"
                     /translation="MTPRYRLVAGLVAGLLAPLSLTGLSLIAMPGSAIAQTTTWTANE
                     GDWGTASNWSNGEPTAGVDAVVNTTASVMVTQGGEICRGLTLSGPLGARLDLTGGALT
                     ADHVSTGTGGTIGISATGNAVFSVTHSLTVGTFSDMVANGGITQIGSLASDSLVVLGT
                     FQIAGTPTLTVSNLALHNGSVYSILIPFTGITGLVTTGTAVLNGKLLVQDFSFSSADV
                     TYEIIHANQIVGTFDEVQLPATGNWSWRIEGNSVFITRGTVATEPATWSDLKAGAGRP
                     AGARGITGR"
     gene            complement(4064..4906)
                     /locus_tag="VF720_02320"
     CDS             complement(4064..4906)
                     /locus_tag="VF720_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEX7878213.1"
                     /translation="MTSGDVQIAWQVMKWPMVACLVLPPLLVYMGLHVVEREVIFVDL
                     ALAQVATLGTCVALIMGYDFESPVAFWISLGVTFLAAALFSWSRRTNRRHVPQEAIIG
                     ITFVVAAAAVILLLSRVAGGKEELENLLTGDVLNVTRGEVGQRAILFALLAGFYGTFH
                     KRFALISSNPDVALAQGLCVRVWDFLFYAAFALVVVSFVRVAGVLLTFAYLIVPAVCA
                     VMLASGWRRRLLIGWAIAATASLLGLWASYSLDLPTGAAIVCACGLLLILVSVYGAVR
                     GAPA"
     gene            complement(4903..5829)
                     /locus_tag="VF720_02325"
     CDS             complement(4903..5829)
                     /locus_tag="VF720_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX7878214.1"
                     /translation="MKTMKLFAVAIAALLTVTGAGTSIAGKLNVVATTPDLASIAMEI
                     GGDAVDVKALAKPTEDPHFVDAKPSLIVTLNKADVLIEGGAELEVGWLPPLLESARNE
                     KIAAGAPGLVVASQGIRMLEVPATLDRAKGDVHALGNPHFMIDPMNVKVVAAVIADHL
                     TQAAPASAETFKANLAAFNSRLDAKTAEWQKLLEPYAGARIVTFHNDFVYLAERFKLD
                     VIATLEPKPGISPSPAHLADVIEKMKSQGAHVILVQPYQNHKTADAVARQTDAVVLES
                     AQQPGAFKDTGTWFLMMDHLVMQLAAALKEKA"
     gene            complement(5846..>6703)
                     /locus_tag="VF720_02330"
     CDS             complement(5846..>6703)
                     /locus_tag="VF720_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878215.1"
                     /translation="PLNLQLKGGQYFAEFGRQNPQHPHAWSFVDQPLVLNRMFGPEGL
                     RSQGARISWLLPTSWYTEAMLSVANSAGGTTFSFRSDESSDIHAGVPDERPVEGPDDL
                     LFVPRLSTSFDLASTQTVVLGASAAFGPNNSGPDARTTIFGADLYWKWKSATADRGFP
                     FVSSQTEVLWRRYDAAERLSADDPDITLPEETLEDRGFYTQFLWGLKPLWVAGLRGEY
                     VHAGDAAFTSPLRTDRYRISPDLTWYLSEFSKLRLQYNYDHRDDQGIDHSVWLQAEFL
                     LGAHSAHKF"
BASE COUNT         1150 a         2321 c         2098 g         1134 t
ORIGIN      
        1 atacggcaac tacctggcca acagcgccgt ctcgcacctg tatttcaagg acgggcggat
       61 ggagatgttc gaattcatcg ataccaagac ccaggagtcg ttccaggtga cgaatcccag
      121 cgaccgcctg gccctccagt cccgcccgcc ggaaaccaag accggcgggg gcggttctca
      181 ctgaggccac ccgccctccc ggcggatccc gcctcttccc caggaaatac ggaaggaacc
      241 gtccgaatgc cgccatccag tgacatgcgc cgaccgggta agccggccgg gcccctcgcc
      301 gcgttcgtcc tcgccaccct cgtggcggga tcctccctca tcgccgagct tgtccgcccc
      361 accgcggcgg ccgccggcgg aaaccccaac gccaagatcg tccttcacct ggtgccctgg
      421 aataaccgcc acacctgcgc ggcaggcgaa ctcgccggcc ccgggcaggt cgtcaccaag
      481 ggagatcttt tcccggcccg gtacgcggcg tacgccctga tcgtggacgg caaccgtggg
      541 gaaggcattt ccggcgtcca gttcgggatc gccttcaatg acacgctgaa gaagggcgtg
      601 gacatcatcg actggcagga atgcagtctc ttcaactggc cgatgcccgg ctggcccgtg
      661 gaaaccgcca ccggcaacct gctcacctgg aacgtcgtca ccgattgcga caccaccggg
      721 ttccgcgtgg ccggcttctt ttacctcacg gcctattcgc ccgaccggct gcggatcatt
      781 cctcgcccgg ccgacaacat ggcggcggtg atcgactgcc tgaagaacgg ctccaagaac
      841 gaggattgcc tggacgtcat tccccaggag aacctgggct tcgtcgattt cggcgggggc
      901 gatgggtaca acccgtggga tccgaagcag aacctcatga agttcaagaa tcccggcggg
      961 gttcgcatcc gcgaggggac tacgccgccc gacaagaaac ccggcggtcg ctgaccgatc
     1021 gtggccgccg cacaactccc cgtctactcg ctgttgctct tcgcggccgc cgccttcggg
     1081 ggtttgctcc ccacgctgcg cccggagctc accaagaaga gccgcgcgtt catcctctcc
     1141 ctggcgtcag gactcctgct cggcacggcg ttcctccaca tgctgccgga agcggtgaac
     1201 atcgtcgggg ccagcgtggt ggtggcggtg ctggtcggct tcgtttcgct cctcctcctg
     1261 gagaagttcg tcatggtcca cccatgcgag gagatggggt gccacttcca tcacctcgga
     1321 acgtcggcct tcctcgggat ttcgctccac agcttcatgg acggcgtggc gctcggcgcc
     1381 agcctgtcgg atgcgggcct ctccctcgtg gtgttcgggg ccatcctcct ccatgagatt
     1441 ccgtgcggtt tctcgctggg gagcctcctc gtcctcggcc ggcgcgaccg gggccgcgtc
     1501 atcgccctca tcctcctcta tgccatcatg acgccgctcg gggcgatcct tacctggctc
     1561 ctttcgctcc agctcccacc caccacggca ggctgggctc ttggcttctc ggccggcaac
     1621 ttcctcttcg tggccacggc cgacctcctg ccacagctgc acctgcacga acgcgccaac
     1681 cggcggcaga tcgtctggtt gctggcgggg ctcgggattt cgtggctcgg gttgctactg
     1741 gaagggtgac gaacgccctc ggtcctgcgt gccggtgagc gatggacggt ttgaatcgca
     1801 gtccaacgca ttggacaatg gccgaaatct gaatccactc ggaggttgct tatgctcgca
     1861 tggcgtcaac gaaagactca attcgccgct ctgctcgggt tcctcgccct gctcccgctc
     1921 acggctgctc cggccggcgc ccaggcgacc aactggaccg gcaccctcgg caactggagc
     1981 ctcggctcca actggagtgc gggcgaaccg aacgccaccg tcgatgcggt gttcaaccag
     2041 ctcggcacgg ccacggtcaa catgaccaac gagtcgtgcc ggaacctcct tatcgggaat
     2101 gacggcgtct cggcgtcggt gaccgtccag gcgccgggaa cactggcggt cgtcggctcg
     2161 atcagggtgc cgtaccagct gtccggcgcc ttcaaccacc agagcggcct ggtcacctgc
     2221 gactcgcttc tgatcggcaa cgtcccgttg aagatcggcc agtacctgat gcagggtggc
     2281 aacctcacgg tgggtacggt gcagatgggg atggtgcaga actccgctgc cacgttctcg
     2341 atcggcgggc tcaacaccca cgtgagcgtg ggccactcgc tctatgtcgg tcgcggcggc
     2401 aacctcgtct ccggaggcgg caccttcacg gtcggttcga tgaacaccga cagcctcgtg
     2461 gtcatcggtt ccttccagat ggtcaaccag ccgaccgtga acgtcaccaa tttcgtgctc
     2521 cggaatctgt ccggcatcac ctacaccttc ctccccaccg gaatcacccc cgtgatttcc
     2581 agcggcgccc tcatccttga cggaacactg accgtcttcg acgcgggcgc gcccaacggc
     2641 acctacgaaa tccttcgcgg caattcgatc tcggggacgt tcgacaccgt cacgctcccc
     2701 ctggtcgggg actggagctg gagaatcgat gggaattccg tcttcgtgag caaggggatc
     2761 gtggcggttg agcccaccac ctggagcgac gtaaagaccc gcagcgtcag gaattagccg
     2821 ccgcggacct cgttcgtcgt gtcatggtcc gaaccgggca ggaatttcgc gttttgccct
     2881 gacggcatga cggatcgtcc gatggtcgga cgcccccgcc ccgccgaacc ggaggaacgc
     2941 gccatgacgc ctcgctatcg tctcgtggcc ggcctggtgg ccggcctcct cgccccgctg
     3001 tcgctgaccg gattgtcgct cattgcgatg cccggctcgg cgatcgccca aaccaccacc
     3061 tggaccgcca acgagggcga ctggggcacc gcctccaact ggtccaacgg cgagcccacc
     3121 gccggcgtcg acgccgtggt caacaccacg gcgtcggtca tggtgaccca gggaggcgaa
     3181 atctgccgcg gcctcacgtt gagcggtccc ctcggtgccc gcctcgacct gaccggcggc
     3241 gcgctcaccg cggatcacgt gagcaccggc accggcggca cgatcgggat cagcgccacc
     3301 ggcaacgccg tcttcagcgt cacccactcg ctcacggtcg gcaccttctc cgacatggtg
     3361 gccaacggcg gcatcaccca gatcggctcc ctcgcgagcg acagcctcgt ggtcctcggt
     3421 acgttccaga tcgccggtac gcccacgctc accgtttcga atctcgcgct ccacaacggt
     3481 tccgtctact cgatcctcat ccccttcacc gggatcaccg gcctcgtgac gaccggcacc
     3541 gccgtcctca acggcaagct gctcgtccag gacttctcgt tctcgagcgc cgatgtcacc
     3601 tacgaaatca tccacgccaa ccagatcgtc ggcaccttcg acgaagtcca gctccccgcc
     3661 accggcaact ggagctggcg aatcgaggga aactccgtct tcatcacgcg cggcacggtg
     3721 gcgaccgaac ccgcgacgtg gagcgacctc aaggcggggg cggggcgtcc ggcgggagcg
     3781 cggggtatca cggggcggta ggcggctggg actgacgtcg gaacagtaat gtcgaaaatc
     3841 tgaaatcgga attctggatc atgttgccga ctcggctctg ccgagtcggc actttcattt
     3901 cagtttgaca tcgtcatgac gagcgtgacg gagggagagg cggatgggat catcgccgcg
     3961 ctgacgcctc ccccggctca atcgcgaatt caagcctgga tcaaggagcg gccaatcctc
     4021 ccgccgtcgc ccagcgtgca aggtttccga ctcggccccc ccctcacgcc ggcgcccccc
     4081 gaaccgcccc gtacacactg accagaatca acagcaaccc gcacgcgcac acgatggccg
     4141 ctccggtcgg caggtccagg ctgtacgaag cccacagtcc gagcaggctg gcggtggcgg
     4201 cgatggccca gccgatgagc aatctccttc gccagccgga ggccagcatc acggcgcaga
     4261 cggcgggcac gatgagatag gcgaacgtca ggagcacacc ggccactcgc acgaaactga
     4321 cgacgaccag cgcgaacgcc gcgtagaaca ggaagtccca aacccgcacg caaaggccct
     4381 gggccagtgc gacgtcaggg tttgaggaaa tcaacgcgaa gcgcttgtgg aaggtgccgt
     4441 agaaccccgc gagcagggca aagaggatgg cgcgctggcc cacctcgccc cgcgtcacgt
     4501 tgaggacatc gccggtcaat agattttcga gttcttcctt tcccccggcc acccggctca
     4561 gaagcaggat gacggccgcg gccgccacca cgaacgtgat gccgatgatg gcctcctgcg
     4621 gaacgtgacg ccgattggtg cggcgggacc agctgaagag ggccgcggcc aggaaggtga
     4681 cgccgagcga gatccagaag gccaccgggc tctcgaagtc gtaacccatg atcagggcca
     4741 cgcaggtgcc aagggtggcc acctgggcca gcgccagatc gacgaagatc acttcgcgct
     4801 cgacgacgtg gagccccatg tacaccagga gcggcggcag cacgaggcac gccaccatgg
     4861 gccacttcat cacctgccac gcgatctgaa catcgccgga tgtcatgcct tctccttgag
     4921 ggccgcggcc agttgcatga ccaggtgatc catcatcagg aaccaggtgc cggtgtcctt
     4981 gaaggcgccc ggctgctggg cgctctccag cacgacggcg tcggtctggc gcgccacggc
     5041 atcggccgtc ttgtggttct ggtagggctg cacgaggatc acgtgggccc cctgcgactt
     5101 catcttctcg atgacgtccg ccaggtgcgc cggcgagggc gagatccccg gcttcggctc
     5161 gagcgtggcg atgacgtcga gcttgaaccg ctcggccagg tagacgaaat cgttgtgaaa
     5221 cgtcacgatc ctggccccgg catagggttc gagcagtttc tgccactcgg ccgtcttcgc
     5281 gtcgagccgc gagttgaacg ccgccaggtt ggccttgaac gtctccgccg aggccggggc
     5341 ggcctgcgta aggtgatcgg cgatgaccgc ggcgacgacc ttcacattca tcgggtcgat
     5401 catgaagtgc gggttgccca gcgcgtggac atcccccttc gcgcgatcga gcgtggcggg
     5461 cacctcgagc atccggatcc cctgagaggc caccacgagc cccggggctc ccgcggcgat
     5521 cttttcgttg cgcgcgctct cgaggagcgg cgggagccag ccgacttcga gctcggcgcc
     5581 cccctcgatc agcacgtcgg ccttgttcag cgtgacgatc aggctgggct tggcatcgac
     5641 gaagtgaggg tcttcggtcg gcttggccag cgccttcacg tccacggcgt cgccgccgat
     5701 ctccatggcg atcgaggcca ggtcgggcgt ggtggccacg acgttcaact ttcccgcgat
     5761 cgatgtcccg gcgccggtta ccgtcagcag cgcggcgatg gccaccgcga acagcttcat
     5821 ggtcttcatc ggatgtccac tccctctaga atttgtgtgc ggagtgcgcg ccgaggagga
     5881 actcggcctg caaccacacc gaatggtcga tgccctggtc gtcacggtgg tcgtagttgt
     5941 attgcagccg cagtttcgag aactcgctca ggtaccaggt gaggtcgggc gagatgcggt
     6001 agcgatcggt gcggagcggc gacgtgaacg cggcgtcccc ggcatggaca tactcgccgc
     6061 gcagccccgc cacccagagg ggcttcaggc cccacaggaa ctgcgtgtag aagccgcgat
     6121 cctcgagcgt ctcctccggg agcgtaatgt cgggatcgtc ggccgacagg cgctccgccg
     6181 cgtcataccg gcgccacagc acctccgtct gggaggagac gaacgggaag ccgcggtcgg
     6241 ccgtcgccga cttccacttc cagtagaggt ccgctccgaa gatcgtcgtc cgggcgtcgg
     6301 ggcccgagtt gttcggaccg aaagccgccg aggcgcccag cacgaccgtc tgcgtgctcg
     6361 ccaggtcgaa cgacgtcgag aggcgcggca cgaacaggag gtcgtccggc ccctccaccg
     6421 ggcgttcatc cggcacgccg gcgtggatgt ccgacgactc gtccgagcgg aagctgaacg
     6481 tcgtcccgcc ggccgagttg gcaacgctca acatcgcctc ggtgtaccag gaggtcggca
     6541 ggagccatga gatccgcgcg ccctgactgc gcagcccttc gggcccgaac atccggttca
     6601 gtaccagcgg ctggtcgacg aacgaccagg cgtggggatg ctggggattc tggcggccga
     6661 actccgcgaa gtactgcccc cccttcaatt gcaggttcaa ggg
//
LOCUS       DASVOD010000120         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_634026, whole genome shotgun sequence.
ACCESSION   DASVOD010000120 DASVOD010000000
VERSION     DASVOD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634026"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..937
                     /gene="murQ"
                     /locus_tag="VF720_02335"
     CDS             17..937
                     /gene="murQ"
                     /locus_tag="VF720_02335"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEX7878216.1"
                     /translation="MAHRRVFEEVSGLLTEQRNPRTADLDRIPVRAVLERINAEDRLV
                     AEAVAGEIPWIEQAVERVVAAFRAGGRLIYAGAGTSGRLGVLDAAECPPTFGTPPEMV
                     RGVIAGGLPALTGAIEGAEDRGEEAVAALADLNVGPNDVVLAIAASRRTPYALAALEE
                     GRRRGAATVFLTTNPRASLTVDVDVAICPVVGPEALMGSTRMKSGTAQKLVLNMITTA
                     SMVRMGKVYENMMVDLMATSRKLVERSRRTVMIVTGLDYDAAAQAIDAAEGSVKTAIV
                     MTRAGVDLGEARRRLDAADGMVRGAIGENG"
     gene            946..>1716
                     /locus_tag="VF720_02340"
     CDS             946..>1716
                     /locus_tag="VF720_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX7878217.1"
                     /translation="MSGRTWGAFRGVVAAALVVVLAIAGPGCGGGGAKSGGPVVVDFW
                     QFWEGDVITPILTRFEAENPGIKVNMKQLTWQNGREEILAAVAANTAPDLCELGSTWF
                     GRFAADGALADVTADTDSLRARLRQWEAATYDGKVYGVPWVLGTRAVFYNKKLFRAAG
                     LNPDLGPPDTWPRLVEAATKIHKPADGVYGFGRNSGERYVLFKKFMPLAWSNGGDVLS
                     VDGTECVFDSPANLEALKFWLSLKSVSLTERQDQIDQAF"
BASE COUNT          262 a          546 c          652 g          256 t
ORIGIN      
        1 ggacataata accgagatgg cccaccgtcg tgtcttcgag gaagtttcgg ggttgctcac
       61 cgagcagcgg aatccgcgca cggccgatct cgatcgcatc ccggtgcggg ccgtgctgga
      121 gcggatcaac gccgaagacc gtctcgtggc ggaggcggtg gccggtgaaa tcccgtggat
      181 cgagcaggcg gtcgagcggg tcgtggccgc ctttcgtgcc ggcggacggc tgatctacgc
      241 cggcgccggc acgtcggggc gcctgggcgt tctcgacgcg gccgagtgtc cgcccacgtt
      301 cggtacgccg ccggagatgg tccgcggcgt catcgcgggg gggctgccgg ccctgacggg
      361 cgccatcgaa ggggccgagg accgggggga ggaggcggtg gccgcgctgg ccgacctgaa
      421 cgtggggccg aacgacgtgg tcctcgccat cgccgccagt cgccggacgc cctatgccct
      481 ggcggcgctc gaggaagggc gccgccgcgg ggccgccacc gtcttcctca ccacgaaccc
      541 gcgcgcttcg ctcacggtgg acgtggacgt ggccatctgc ccggtcgtcg ggcccgaggc
      601 gctgatgggc tccacccgga tgaagtcggg cacggcgcag aagctcgttc tcaacatgat
      661 cacgacggcg tcgatggtgc ggatggggaa ggtgtacgag aacatgatgg tcgacctcat
      721 ggccaccagc cggaagctgg tggagcggag ccgccgcacg gtgatgatcg tgaccggcct
      781 cgattacgac gcggcggcgc aagccatcga tgccgcggag ggaagcgtga agaccgccat
      841 cgtgatgacg cgggcgggtg tggatctggg cgaagcccgc cggcggctcg acgcggcgga
      901 cggcatggtg cgcggcgcca tcggggagaa cggatgacgg gtcggatgtc cggtcggacg
      961 tggggagcct ttcgcggggt ggtcgcggcg gcgctcgtcg tcgtgctggc gatcgcgggg
     1021 ccaggatgcg gtggaggggg tgcgaagtcc ggcggcccgg tcgtcgtcga tttctggcag
     1081 ttctgggaag gggacgtgat cacgccgatc ctcacccggt tcgaggccga gaaccccggc
     1141 atcaaggtga acatgaagca gctcacctgg cagaacggcc gcgaggagat cctggccgcg
     1201 gtggccgcca acacggctcc cgacctgtgc gagctcggct ccacctggtt cggccgcttc
     1261 gccgccgacg gggcgctggc cgacgtgacg gccgacacgg actcgctccg cgcgcgcctg
     1321 cgccagtggg aagccgccac gtacgacggc aaggtgtacg gcgtgccgtg ggtgctcggc
     1381 acgcgggccg tcttctacaa caagaagctc ttccgggccg ccggactcaa tcccgacctg
     1441 gggcctcccg atacgtggcc gcgccttgtg gaggccgcca cgaagatcca caagccggcc
     1501 gatggggtgt acggcttcgg ccggaacagc ggcgagcgct acgtgctctt caagaagttc
     1561 atgccgctcg cctggagcaa cggcggcgat gtcctctcgg tggacggcac ggaatgcgtg
     1621 ttcgattcgc cggccaacct cgaggcgctg aagttctggc tgagcctcaa gtcggtgtca
     1681 ctgaccgagc ggcaggacca gatcgatcag gccttc
//
LOCUS       DASVOD010000121         7300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_529830, whole genome shotgun sequence.
ACCESSION   DASVOD010000121 DASVOD010000000
VERSION     DASVOD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529830"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1752
                     /locus_tag="VF720_02345"
     CDS             <1..1752
                     /locus_tag="VF720_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878218.1"
                     /translation="CLSVAMPADVRVEFDSCFLTPDGRTVVVLGKPRNPDGTVSAASR
                     VYVRTLDGYDYKELPGTEGALYSVPSPDGRSLYFLAPSAPGSTQLRVSRLPIDGSAPP
                     TTICQWKDSWRWMAPQESGDLLLGESPTSFIRLPGNGGAPSAPVAIDCGRPGVSRYDP
                     PGGFLPDGRHMFINVVLYDARGWHYSLGVMNLVDGKVRILDEDGGNGFYLPSSGHLVF
                     SRGNTILGVPFDAEGLTMRGAPTAVWSGLASYATYVPATFDLTPDGDLFYRPGQLGGD
                     RPIVLVDAAGHATPWSSGPRPMNFAPIPSRDGSRIATQFISARGIDEIWVSSAGRDDF
                     HRVGTEPGADAYFPVWSPDQRQIAYRRNGSDDKDGLYIIGVDGGEMTRILPEPAGTDY
                     YDPNSWLPDGSGILLTRLVQGKSSIWLLPKDGGQWGSPRSILGGEFRNELPMISPDGR
                     ALAFQNDESGKLQSYVVAFRGQGVVGRAIAIRTEGSRWHQWGNDGTTLYVHDERNRLM
                     KVTFTTTSELSVSAPQEVCDLDKQHGNSIMWTVLPDGRFFVGLKNAHEEDATSANLVL
                     GWSRVLAGGAGKTDRNH"
     gene            1848..2648
                     /locus_tag="VF720_02350"
     CDS             1848..2648
                     /locus_tag="VF720_02350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7878219.1"
                     /translation="MDDHQIALTERYNREAASYRDLWAPVLHVPARALVEALRGPAAR
                     VVDLGSGVGSLLPTLKGAFPQASILGIDQSIGMLSLAPPEFPRAVMDASRMALADRSV
                     DVVVMAFMLFHLPEPLAGLREARRVLRGRGRVGTLTWVDELRSPAITCWSRCLDDHGA
                     PELDPATKARHEPVNSTSKVIGLLRAAGFEEARAWDEEIVARFDLGHFYSLKTSIGAD
                     YTRFMGLEEPIRARFLEEVRRRLEALAPADFHATTRVVFGVGRIPGND"
     gene            complement(2656..3060)
                     /locus_tag="VF720_02355"
     CDS             complement(2656..3060)
                     /locus_tag="VF720_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006036909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7878220.1"
                     /translation="MDITRAGSTPSNRGPADYFTGSVRLDPLFQAADPARAVGAMVTF
                     EPGARTAWHTHPLGQTLIVTSGAGLAQRWGGPIEPIRPGDVVWFPPGEKHWHGASATT
                     GMTHIAIQERLDGRTVDWLEKVTDEQAGQAAG"
     gene            complement(3107..4339)
                     /locus_tag="VF720_02360"
     CDS             complement(3107..4339)
                     /locus_tag="VF720_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007509039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7878221.1"
                     /translation="MDVTTVVIGAGHAGLAMSHCLTSRSIDHVVLERGEVANSWRTER
                     WDSLRLLTPNWQSRLPGHGYEGDDPDGFRTMPETVNFLERYAERCGAPVVTQTNVTAV
                     RRTDDGYHVVTNRGEWRARTVVMASGACNIPSVPPAAAALPCSITTITPNHYRNPDSL
                     PSGGVLVVGASATGTQIAEEIHRSGRPVTLAVGEHIRAPRTYRGRDIKWWMDAAGVMD
                     DRYDEQDDLNRARNVPSLQLAGSTDRRTVDLNRLTDLGVRLVGRFAGLKDGRAQFSGS
                     LFNQCALSDLKMNRLLDRIDEWASANGLDVQTDPPHRFEPTRVNGTPPLGLDLTSGEI
                     RTVLWATGYHPDYSWLDVPVLDRKGRVRHEGGIVDSPGLYLMGLPFLRRRKSTFIDGA
                     KDDAQEICAHLSNYLDGE"
     gene            complement(4363..4923)
                     /locus_tag="VF720_02365"
     CDS             complement(4363..4923)
                     /locus_tag="VF720_02365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010628212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7878222.1"
                     /translation="MSTTKNPVDNGVNVQALLDARKALTDAPEAAKFKWRARCEWQKG
                     THSRSTVEGFYGLGEEQRHRTKFTFEADHPELFASEDNGATPVEIVLAGLASCLTAGI
                     AAVAQHRDIQLRRVTATIEGGMDVQGILGIDSDVRNGFDGIKVIYDIDADATPDEIRA
                     IVAQSQKRSAVYDVITNPTNVTVEVK"
     gene            complement(4920..6104)
                     /locus_tag="VF720_02370"
     CDS             complement(4920..6104)
                     /locus_tag="VF720_02370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7878223.1"
                     /translation="MQETPSIIQERYDAIVVGARCAGAATAMLLARLGLSVLLIDRDR
                     HGADTLSTLALMRAGVLQLHRWGLLDRIVAAGTPPITRTSFIYGDETITVPIKSRNGV
                     DALYAPRRTVIDAMLAGAAAESGVEVAHGARLLDLERNAEGRVVAARIEPKGGAARRV
                     GAGIMIGADGRRSTVARLVEARTERSRPDACGVVYGFFPGLENDRSRWYYAPRIAAGS
                     IPTNNGNACVFLSMPPARFHGEVRADMDAGYRRGLAECTAELAGEVADKEPSEPLRGF
                     PGEPGYIRQSHGPGWALVGDAGYFKDPITAHGISDALRDAELLARAVVRGTDDALAGY
                     QTTRDELSRDLFEITHDIAGFGWRMDDLKPLHLQLSHSMNREVEALLGLDAPVPHAGR
                     TP"
     gene            complement(6128..6565)
                     /locus_tag="VF720_02375"
     CDS             complement(6128..6565)
                     /locus_tag="VF720_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX7878224.1"
                     /translation="MTGGSEDVWGVPVPVVGQVAERTRKVAPGDIERFTDISGDRNPL
                     HYDEAVAKASRFGTIVVQGGVTSAILNAVVAEDLPGPGTVFLEVHWRFIAPVRPGDSI
                     TGRVEVAEVRHDKPITKLKTRVTRDDGTVVLEGDAVCYTMKLT"
     gene            complement(6673..>7300)
                     /locus_tag="VF720_02380"
     CDS             complement(6673..>7300)
                     /locus_tag="VF720_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878225.1"
                     /translation="MAQKHHGDLVVNLGSSPAYIHVGFTNCPNPAPWQILGLCPGFSA
                     TLVNEDKSPVLSGALPPGWSGWIKVAADASVPSNTTCCFQVKIVCNGSPGIIDMCATS
                     CTWSVSTVPPVPDKVAFGIFRTAPSPASTGTVIDFVVPTSGPVRVEAFDLSGRQVRLV
                     MTGPVEAGVHSVRWDMRGEGGSPIAPGTYFIKLSAAGQTSTRKIVLVR"
BASE COUNT         1093 a         2484 c         2472 g         1251 t
ORIGIN      
        1 tgcctctcgg tggccatgcc ggcggatgtc cgtgtggagt tcgacagctg cttcctcacg
       61 ccggacggcc ggacggtggt cgtgctggga aagccccgga acccggacgg cacggtgtcg
      121 gccgcctcgc gcgtttatgt gcgaacgctc gacggttacg actacaagga gctccccggt
      181 accgagggcg ccctctacag cgtgccgagc cccgacggcc gcagcctcta cttcctcgcg
      241 ccatccgcgc ccggctcgac acagctccgg gtgtcgcgcc tgccgattga cgggagcgcg
      301 cctcccacga cgatctgcca gtggaaggac agctggcgat ggatggcccc ccaggagagc
      361 ggcgacctgc tccttgggga aagtcccacc agtttcatac gccttcccgg gaacgggggg
      421 gccccctcgg cgccggtggc catcgactgc gggcgtcccg gcgtcagccg ttacgacccc
      481 ccgggtggat ttctccccga cggccgccac atgttcatca acgtcgtcct ctacgacgcg
      541 cgcggttggc attacagcct tggcgtcatg aatcttgtcg acggcaaggt caggatcctc
      601 gatgaggacg gcggcaacgg tttctatctg ccgtcctcgg gacacctggt cttctcccgc
      661 ggcaacacga tcctgggtgt tcccttcgat gccgagggcc tcacgatgcg aggcgccccg
      721 acggctgtct ggagcggtct cgcctcgtat gccacctacg ttccggccac cttcgacctg
      781 acgcccgatg gcgacctgtt ctatcgtccg ggccagctcg gcggcgatcg tccgatcgtt
      841 ctggtcgatg cggccggcca cgctacccca tggtcctccg ggcctcgacc gatgaacttc
      901 gcgccgatcc cctcgcgcga cgggagccgg atcgccacgc aattcatcag tgcccgaggc
      961 atcgacgaga tctgggtttc ctccgccggg cgggacgatt tccaccgggt gggcaccgaa
     1021 ccgggcgccg acgcctactt tcccgtctgg tcgcccgatc agcgccagat cgcctatcgg
     1081 cgcaacggat ccgacgacaa ggacggcctg tacatcatcg gcgtggacgg cggggagatg
     1141 acccgcatcc tccccgagcc ggccggcacg gactactacg accccaattc atggctcccc
     1201 gacggcagcg gaattctcct cacacgactc gtccagggga agtcgagcat ctggctgctg
     1261 ccaaaggacg gggggcagtg gggttcgccc cgttcgatcc tggggggcga attccgcaac
     1321 gagctgccga tgatttcgcc ggacggccgt gcgcttgctt tccagaacga tgagtcggga
     1381 aagcttcagt cgtacgtcgt ggccttccgc ggccagggcg tggtcggccg ggccattgcg
     1441 atccggacgg agggtagtcg ttggcaccag tggggcaacg acgggacgac gctctatgtc
     1501 cacgacgagc gcaatcgcct gatgaaggtg acgtttacga caacctccga gttgtccgtc
     1561 tccgctcccc aggaagtctg cgacctggac aagcagcacg ggaactccat catgtggacg
     1621 gtgctccccg acggcaggtt cttcgtcggc ttgaagaacg cccacgagga ggacgccacg
     1681 agcgccaacc tggtgctcgg gtggagccgc gtgctggcgg gcggggccgg aaaaaccgat
     1741 agaaaccatt aagtaatagc tacttaaaag acccttcgga aaattctacg ctcgcgggcc
     1801 cctcattgaa gagggtgtga gcacgccggc caccccgcgg gacattcatg gacgaccatc
     1861 agatcgcact caccgagcgg tacaaccgcg aagcggcctc ctaccgggat ctctgggcgc
     1921 cggtgctgca cgttccggcg cgtgccctcg tcgaggcact gcgtggtccc gccgcgcggg
     1981 tggtcgacct cggcagcggc gtcggcagcc tgttgccgac gttgaagggc gcctttccgc
     2041 aggcatcgat cctgggaatc gaccagtcga tcggcatgct ctcgctggcg ccgccggaat
     2101 ttccacgcgc cgtcatggac gcctcgcgca tggcactggc cgatcgctcg gtggatgtgg
     2161 tggtcatggc cttcatgctg ttccatctgc cggaaccgct ggccgggctg cgcgaggcgc
     2221 gccgcgtgct gcgcgggcgg ggacgggtgg gcacgctcac gtgggtcgac gagctccggt
     2281 cgcccgccat cacctgctgg tcgcggtgcc tcgacgatca cggcgccccc gagctcgatc
     2341 cggccacgaa ggcgcgacac gagccggtca attccacgtc aaaggtcatc gggctgctcc
     2401 gggccgccgg gttcgaggag gcgcgggcgt gggacgagga gatcgtggcg cggttcgacc
     2461 tcgggcattt ctacagcctc aagaccagca tcggcgcgga ctacacacgc ttcatgggtc
     2521 tcgaagagcc catacgcgcc cgtttcctcg aggaggtgcg ccgccggctc gaagcgctcg
     2581 caccggcgga cttccacgcc acgacacgcg tcgtgttcgg cgtggggcgc atccctggta
     2641 acgactgagg cggcgtcagc ccgctgcctg ccccgcctgc tcgtcggtca ctttttccag
     2701 ccagtccacc gtccgcccgt ccagacgttc ctggatggcg atgtgcgtca tgccggtcgt
     2761 cgcgctcgcg ccgtgccagt gcttctcgcc ggggggaaac cagaccacat cccccgggcg
     2821 tatcggttcg atcggcccgc cccagcgctg ggccagtccg gcgccggagg tcacgatgag
     2881 cgtctgcccc agcgggtgcg tgtgccacgc cgtgcgcgcg cccggctcga acgtgaccat
     2941 ggcgccgacg gcgcgcgccg ggtcggccgc ctggaaaagg gggtcgagcc ggacgctgcc
     3001 ggtgaagtag tcggccggtc cgcggttgga cggggtggag ccggcgcgtg tgatgtccat
     3061 gaggttcctc gtggtttgag gtcagggcgt cggtggcccc ggtcgttcat tctccgtcga
     3121 gatagttgga caggtgcgcg cagatctcct gcgcgtcgtc cttcgcgccg tcgatgaatg
     3181 tcgacttccg ccgcctgagg aaggggaggc ccatcaggta caggccgggg gagtcgacga
     3241 ttcctccctc gtgtcgcaca cgccccttcc ggtcgagcac cggtacgtcg agccacgaat
     3301 agtcgggatg atacccggtg gcccagagca cggtgcggat ctcgccgctc gtaaggtcga
     3361 ggccgagcgg cggcgtgccg ttgacccgcg tcggctcgaa ccggtgcggg ggatcggttt
     3421 ggacgtcgag gccgttggcg ctggcccact cgtcgatccg gtcgagcagg cggttcatct
     3481 tgaggtccga cagggcacac tggttgaaga gggagccgga gaattgcgcc cggccgtcct
     3541 tcaggccggc gaaccgtccg accaggcgca cgccgaggtc ggtgaggcgg ttgagatcca
     3601 cggttctccg gtcggtcgat cccgccagtt gcagcgacgg cacgttgcgg gcccgattca
     3661 ggtcgtcctg ctcgtcgtag cggtcgtcca tgacgccggc ggcatccatc caccacttga
     3721 tgtcgcggcc gcggtaggtg cgcggggcgc ggatgtgctc gccgaccgcc agcgtcaccg
     3781 gccgtcccga gcggtggatt tcctcggcga tctgcgttcc ggtggccgag gcgccgacca
     3841 ccagcacgcc gccggaaggg agcgagtcgg gattgcggta atgattgggc gtgatcgtcg
     3901 tgatgctgca ggggagtgcc gcggctgccg gcggcacact ggggatattg catgcccccg
     3961 aggccatgac gaccgtccgg gcgcgccatt cgcctcgatt cgtcacgacg tgatagccat
     4021 cgtcagtgcg gcggaccgcg gtgacgttcg tctgagtcac caccggggct ccgcatcgct
     4081 ccgcgtatcg ctcgaggaag ttgaccgtct ccggcatggt ccggaaaccg tcggggtcat
     4141 ccccctcgta tccgtggccc ggcagccggc tctgccagtt cggggtaagg aggcggagcg
     4201 aatcccaccg ttcggtcctc cacgagttcg ccacctcacc ccgctcgagg accacgtggt
     4261 cgatcgaacg cgaggtgagg cagtgactca tggcaagccc ggcgtggccg gccccgatga
     4321 cgacggtggt gacgtccatc gatgatcgtg gaatcgcggc ggctacttga cctcgaccgt
     4381 gacgttcgtc gggttggtga tgacgtcgta caccgccgac cgcttctgag actgggccac
     4441 gatggcgcgg atctcgtcgg gcgtggcgtc ggcgtcgatg tcgtagatca ccttgatgcc
     4501 gtcgaagccg ttgcgcacgt cgctgtcgat gcccaggatc ccctggacgt ccatcccgcc
     4561 ttcgatcgtg gccgtcacgc ggcggagctg gatgtcgcgg tgctgggcca ccgcggcgat
     4621 gccggcggtc aggcagctcg ccagcccggc cagcacgatc tcgaccggcg tggcgccgtt
     4681 gtcctccgag gcaaacagct ccggatggtc ggcctcgaac gtgaatttcg tccggtgacg
     4741 ctgctcctcg ccaaggccgt agaatccctc caccgtcgag cggctgtgcg ttcccttctg
     4801 ccactcgcag cgggcccgcc acttgaactt ggccgcctcc ggggcatcgg tcagggcttt
     4861 gcgggcatcg aggagcgcct ggacgttcac accgttgtcg accgggtttt ttgtcgtgct
     4921 catggggtcc tccctgcgtg tggcaccggg gcgtcgaggc cgagcagggc ctccacctcc
     4981 cggttcatcg agtgactcag ttgaagatgg agcggcttca ggtcgtccat ccgccagccg
     5041 aagccggcga tgtcgtgggt gatctcgaag aggtcccgcg acagttcgtc gcgggtcgtc
     5101 tggtatccgg ccagtgcgtc gtccgtgccg cgcaccaccg cgcgggccag gagctcggcg
     5161 tcgcgcagcg catcgctgat cccgtgtgcc gtgatcgggt ccttgaagta gccggcgtcg
     5221 ccgaccagcg cccaccccgg gccgtggctc tgccggatgt agcccggttc ccccgggaag
     5281 ccccgcagcg gttccgatgg ctccttgtcg gccacttcac cggccagctc ggccgtgcat
     5341 tcggccaggc cccggcgata accggcgtcc atgtcggccc gtacctcacc gtggaacctg
     5401 gccggcggca tcgagaggaa gacgcaggca ttcccgttgt tcgtcgggat cgagccggcc
     5461 gcgatccgcg gggcgtagta ccagcggctg cggtcgttct cgaggccggg aaagaagccg
     5521 tacaccacgc cgcaggcatc cgggcgcgaa cgttccgtgc gggcctcgac caggcgggcc
     5581 accgtggacc gccgcccgtc ggcgccgatc atgatgccgg cgcccactcg tcgggcggcg
     5641 ccacccttcg gttcgatccg cgccgccacg acccgcccct ccgcattacg ctcgaggtcg
     5701 agcagccgcg ccccgtgcgc cacttccacg cccgattccg cggccgcgcc ggccagcatg
     5761 gcgtcgatca ccgtgcggcg gggggcgtag agcgcatcga cgccgttccg tgacttgatc
     5821 ggcacggtga tcgtttcatc gccgtagatg aagctggtgc gggtgatggg gggcgtcccc
     5881 gcagccacga tccggtcgag caggccccag cgatgaagct ggagcacgcc ggcccgcatc
     5941 agggccaggg tggaaagcgt gtcggcgccg tgccggtcgc gatcgatgag gagcaccgac
     6001 agtcccagcc gcgccaggag catggccgtg gccgccccgg cgcaacgcgc gccgaccacg
     6061 atggcgtcgt agcgctcctg aatgatggag ggggtttcct gcatggtggc gtccccgggc
     6121 tgcggcctca ggtcagcttc atcgtgtagc agaccgcgtc gccttccagc accaccgttc
     6181 catcatcccg ggtgacacgc gtcttgagct tcgtgatcgg cttgtcgtgg cggacttcgg
     6241 caacctccac ccggccggtg atggaatcgc ccggtcgcac tggcgcgatg aaacgccagt
     6301 gaacttccag gaacaccgtc cccggtccgg gcaggtcctc ggccacgacc gcgttcagga
     6361 tggcgctcgt cacgcctccc tgtacaacga tagtgccgaa gcggctggcc tttgccacgg
     6421 cctcgtcgta gtgcagcgga ttccggtcac cgctgatgtc ggtgaaccgc tcgatatcgc
     6481 ctggggccac cttccgggtg cgttcggcca cctggccgac aaccggcacg ggcacacccc
     6541 aaacgtcttc cgagccgccg gtcatggagc gcctttctcg ttcattgtga ggaggagatg
     6601 acggtgccgc tccccccggg cctccggggg gagcggcact gactgtagac cctggcccgc
     6661 gcctgtcaac ggctatcgca cgagcacgat cttccgtgtg gacgtctgcc cggccgcgct
     6721 cagcttgatg aaataagtcc ccggtgcgat gggcgaacca ccctcgccgc gcatgtccca
     6781 tcgcaccgag tgcacgccgg cttccaccgg cccggtcatc accaggcgaa cctgccgacc
     6841 gctcaggtcg aaggcttcca cgcgaaccgg tccgctcgtc ggaaccacga agtcgatgac
     6901 ggttccggtc gaggcgggac tcggcgccgt ccggaaaatg ccgaaggcca ccttgtcggg
     6961 aacgggcggc acggtcgaga cggaccaggt gcaactcgtc gcacacatgt cgatgatccc
     7021 gggcgaaccg ttgcagacga tcttcacctg gaagcagcag gtggtgttgc tggggacgct
     7081 tgcatccgcg gcgaccttga tccaccccga ccagcccggc ggaagcgcgc cactcaggac
     7141 gggtgacttg tcctcgttca cgagcgtggc gctgaagccg ggacagagcc cgaggatctg
     7201 ccacggtgcc gggttcggac agttcgtgaa ccccacatgg atgtaggccg gcgatgagcc
     7261 caggttgacc accaggtctc catggtgttt ctgggccatg
//
LOCUS       DASVOD010000122         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_9100, whole genome shotgun sequence.
ACCESSION   DASVOD010000122 DASVOD010000000
VERSION     DASVOD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9100"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1953
                     /locus_tag="VF720_02385"
     CDS             274..1953
                     /locus_tag="VF720_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878226.1"
                     /translation="MSARPLLLAVALVALVSPVVPGLVTPARAVPFDNTPKILLHLKP
                     VTAKNQCASGITDCRDAVTSGLISTPGDPHFYYALVLGARGNLATLGGFQFGINYDDG
                     CAGGLADHDQIDILGWTLCGMLEFSSPSPRWPSPGSGNLITWDAINSCQTGEHAVAGY
                     FYLSCYNNADVLDIIPRPVDARAVMANCTSVETTLPASSLGSLAFSASGATLGCNSCD
                     GPCPEPPPRTGCPVPPIDHTPPGAVIMTLGTIAGNSIEVYWHAPGDDGFINGPATSYD
                     VRWSTAPITPLNFSSANQASNEPVPAAPGTQQGMTVTNLQISTTYYIAMKSADEQRNI
                     SDLSNVVTARTVDTSGDLNAATSLALHAIPVGPGGCAAGRIPDCRDANTIARLLPDAY
                     LVYLLAGEFAEIGGIEVGIQYSGGAADGAGDQAGVDVYNWTSCADLQFPSPTPAWPAP
                     GSGNLMTWQSTHCQFPPTTVAGYFYVGAYSDDEFRLTIRPGSGQAAVATCNSVETPLP
                     PDALGSVRFTSDGKQAGINPCGRGTPTPVRMSTWSRIKNLGEAKGTKPTGR"
     gene            complement(1981..2646)
                     /locus_tag="VF720_02390"
     CDS             complement(1981..2646)
                     /locus_tag="VF720_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878227.1"
                     /translation="MDRPDRESLKTEWMSFLATGVIAFVIAIAVSGCATAQLDNMWRD
                     ETFVTPMDKVFVVALKEEASRRRLWEDEIVHEMAKHRVTGVPSYSLYPDAVPDTQAVE
                     MAVREQGFDGVMVIFDLDPRQEEVYVPGYLATEPVYRVDPWSRRWLTYYVQVEHPGYY
                     ATDTVIRDQVDLYSTGDRGRLVWSGVGEVVNAGDAHQIREEVCDRIVPELAKQGIIGS
                     GMD"
     gene            complement(2868..>3795)
                     /locus_tag="VF720_02395"
     CDS             complement(2868..>3795)
                     /locus_tag="VF720_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878228.1"
                     /translation="ADVPGAMVVKVGEKVTDGQIVAQSKSFFGMFKSEARANTTGVLE
                     AVSEITGQITLREAPIPVEVDAYVDGTVIEVMPKEGVVVETAGAFIQGIFGVGGETSG
                     KLKRVVESPDQVLDESKLPADCKGLILVGGSQVNRATIQKARERGARGIVVGGIDAEE
                     LRLLLGYDLGVAITGNETIGITVVVTEGFGRLTMAKKTFELLGRMDGQGCSINGATQI
                     RAGVQRPEILVPLLAGQANHAEADFAGGLDIGSSIRVIREPYFGHLGSVTELPPELQA
                     LETESHARVLKVRFADGKEAVVPRANVEMIEG"
BASE COUNT          614 a         1315 c         1232 g          634 t
ORIGIN      
        1 gaccagccaa gcccactcgg agttggcctt gtcgattcgg ccggcgaatt cctcgggagt
       61 tggcacggcg agctcgaacg aggtggcgct gtcggtgatg gccggggcgt agatggccag
      121 gcaggccggg gcgtcgtctg aggtggcggg gcggatcacc ggtaaatcat acgaaatggt
      181 ggcccgattg gctcgtggcc cctatattct ggatggcgcg gccacatcgc ggcccgccac
      241 ccccccctcg actgcccagc ggaggtccca cgcatgtcgg cacgtcctct cctgctcgcg
      301 gtggcgctcg tcgccctcgt ctcccccgtg gttcccggcc tcgtgacccc ggcccgggcg
      361 gtgccgttcg acaatacgcc caagatcctg ttgcacctga agccggtgac ggcgaagaac
      421 cagtgcgcga gcgggatcac cgactgccgg gatgcggtca ccagcgggtt gatttcgacg
      481 ccgggcgatc cgcacttcta ttacgcgctg gtcctcggcg cgcgggggaa cctcgcgacg
      541 ctgggcggct tccagttcgg catcaactac gacgacgggt gcgcgggggg cctggccgac
      601 cacgaccaga tcgatatcct cggttggacg ctgtgcggca tgctggagtt ctcgtcgccg
      661 tcgccgcggt ggccgtcgcc gggatcgggc aacctgatta cctgggatgc catcaattcc
      721 tgccagaccg gcgaacacgc cgtggccggg tacttctacc tctcgtgcta caacaacgcg
      781 gacgtcctcg acatcatccc ccgtccggtc gacgcccggg ccgtgatggc caactgcacc
      841 agcgttgaaa cgacactccc cgccagttcc ctcggcagcc tggctttctc ggcaagtggc
      901 gccacgctcg gctgcaactc ctgcgacggg ccgtgccccg agccgccgcc gaggacgggc
      961 tgccccgttc cgccgatcga ccatacaccg ccgggtgccg tcatcatgac gctcggcacg
     1021 atcgccggga acagcatcga ggtgtactgg catgcgccgg gcgacgacgg cttcatcaac
     1081 gggccggcca cgtcgtacga cgtccgctgg agcacggcgc cgatcacgcc gctcaacttc
     1141 tcctcggcga accaggccag caatgagccg gtaccggcgg cgccgggcac ccagcaggga
     1201 atgaccgtga cgaaccttca gatcagcacg acctactaca tcgccatgaa gagcgccgac
     1261 gagcagcgca acatctcgga tctctcgaat gtcgtgacgg cccgcaccgt ggacacgtcg
     1321 ggcgacctca acgccgccac gtcgctggcg cttcatgcca ttccggtggg accgggcggg
     1381 tgcgcggccg gacgcatccc cgattgccgc gacgccaaca ccatcgcccg cctcctgccc
     1441 gatgcgtacc tggtgtacct gctggccggc gagtttgccg agatcggcgg catcgaagtg
     1501 ggcatccagt acagcggcgg cgcggccgac ggcgcgggcg accaggccgg cgtggacgtg
     1561 tacaactgga ccagctgcgc cgacctgcaa ttcccttcgc cgacaccggc ctggccggcg
     1621 cccggctcgg gaaacctcat gacgtggcaa tccacgcact gtcagttccc gcccaccacc
     1681 gtggccggct acttctacgt gggcgcctac agcgatgacg aattccgcct caccatccgc
     1741 ccgggctccg gccaggccgc cgtggccacc tgcaactccg tcgaaacgcc cctgccgccc
     1801 gacgcgctgg gctccgtgcg gttcacctcc gatggcaagc aggcgggcat caacccgtgc
     1861 ggtcgcggga cgccgactcc ggtgcggatg tcgacatgga gccggatcaa gaacctgggg
     1921 gaggcgaagg gtacgaaacc gaccggtcgt tgacctgaca tccgggggcg ggatggccca
     1981 ctagtccatc ccgctgccga tgatcccctg cttggcgagc tcggggacga tgcggtcgca
     2041 cacttcctcg cggatctggt gggcatcgcc ggcgttcacg acctcgccca cgccggacca
     2101 gacgaggcgg ccgcgatcgc cggtggagta gagatcgacc tggtcacgga tgaccgtgtc
     2161 ggtggcatag tagccggggt gctccacctg cacgtagtag gtgagccagc ggcgtgacca
     2221 gggatcgacg cgatagaccg gctcggtggc caggtagccc ggcacgtaca cctcttcctg
     2281 ccggggatcg aggtcgaaga tgaccatcac gccgtcaaat ccctgttcgc ggacggccat
     2341 ctccaccgcc tgcgtgtcgg gcacggcgtc ggggtaaagg gagtacgacg gaacgccggt
     2401 cacgcgatgc ttggccatct cgtggacgat ctcgtcttcc cagagccggc ggcggctggc
     2461 ctcttccttc aacgccacca caaacacctt gtccatcggc gtcacgaacg tctcatcgcg
     2521 ccacatgttg tcgagctggg ccgtggcgca gccggaaacg gcaattgcga tgacgaaggc
     2581 gatgaccccc gtggcgagaa acgacatcca ttcggtcttc agcgactccc gatccgggcg
     2641 gtccatggct cctccttggg cgtcggcatg ttggccggcg cggtgcaggc aaattgaaac
     2701 catggattgc aagttccttg ccgccccaga agcgcctgtg gcaggacatt tcttcccgaa
     2761 acgacagcgg ggtccggctc gcgtgcggcc ggaccccggg atcgggaaaa tttgggatgg
     2821 gaaacgcccg gactttccaa ggggcgtcac tcgtatgttc gaaattgcta tccctcgatc
     2881 atctccacat tggcgcgcgg caccacggct tccttgccgt cggcaaagcg caccttgagg
     2941 acgcgggcgt gcgactccgt ttcgagcgcc tgcagctcgg gcgggagctc cgtcacgctt
     3001 cccaggtggc cgaagtaggg ctcgcggatg acgcggatcg agctgccgat gtcgaggccg
     3061 ccggcgaaat cggcctcggc atggttggcc tggcccgcca ggagcggcac gaggatttcg
     3121 gggcgctgga cgccggcacg gatctgcgtg gcgccgttga tggagcaccc ctggccgtcc
     3181 atgcggccga gaagctcgaa cgtcttcttt gccatggtca ggcggccgaa gccctcggtg
     3241 accaccaccg tgatgccgat ggtctcgttg ccggtgatgg ccacgccgag gtcgtacccc
     3301 aacaggagtc gaagctcctc ggcatcgatg ccgccgacca cgatgccgcg cgcgccgcgt
     3361 tccctggcct tctggatggt ggcccggttc acctggctgc cgcccacgag gatcaatccc
     3421 ttgcagtcgg cggggagctt cgactcatcg agcacctggt cgggggattc gaccacccgc
     3481 ttcagcttgc cgctggtctc gccacccacg ccgaagatcc cctggatgaa ggcaccggcg
     3541 gtctcgacca ccacgccttc tttcggcatc acttcgatga cagtgccgtc cacgtaggca
     3601 tcgacctcga cggggatcgg cgcctcgcgg agcgtgatct ggccggtgat ttcggatacc
     3661 gcctcgagga cgccggtggt attggccctg gcctccgact tgaacatgcc gaagaacgac
     3721 ttgctctggg ccacgatctg gccgtcggtg accttttccc ccaccttcac gaccatggcc
     3781 cccggcacgt cggcc
//
LOCUS       DASVOD010000123         6160 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_373461, whole genome shotgun sequence.
ACCESSION   DASVOD010000123 DASVOD010000000
VERSION     DASVOD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373461"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /locus_tag="VF720_02400"
     CDS             <1..979
                     /locus_tag="VF720_02400"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878229.1"
                     /translation="TSMTGDDADAIRREAPSVARVAPTTRTVAQIVAGNQNWSTQVQG
                     AGSDYFEVRDWPVEYGLPFTELDVRSAAKVAILGTTVAENLFPGDDPVGQVVRINKVP
                     FSVLGILKKKGGNAMGSDQDDIIVVPYTTSQKRLQGITHIQGMMISAVTKERVPDAQD
                     EITQILRRRHRIAPGGNDDFIIRAQSDIAATAEETSKVMTMLLGAIASVSLLVGGIGI
                     MNIMLVSVTERIREIGIRMAVGARPGDILLQFLLEAIILSTLGGLIGVGLGLAISQIV
                     SKAAGWSVLVAPPAVAMAFGFAVAIGVFFGFYPARKAALLDPIEALRYE"
     gene            complement(989..2920)
                     /locus_tag="VF720_02405"
     CDS             complement(989..2920)
                     /locus_tag="VF720_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7878230.1"
                     /translation="MRNRRLIPPAAMAALFLAAGPSSLPVRPAHAAPADPITAGELSG
                     HVRTLASDEFAGRGTGEAGGEMAARYIADAFKAAGVQPAGDNGGWYQEFPAKVASSLG
                     DGNAVTLPTKKLPHDLELGKEFVPLAFSASGECAAPAIFVGYGITAAEHGYDDYAGLD
                     VSGKVVVCLRYEPARDDTGSIFEGRKDTWHANLRTKARNAETHGAAGIIIVNGPLNEG
                     GADDLLTWEKAGTAQAVGIPAMMITRKGLESIWGLGLPDKPASLKDLQGRIDGEMRPL
                     SQPITSMSVRLKGDIRTETRTTRNVVAWLPPNGGAARMVGRADSTSEPHLVIGAHYDH
                     LGMGGQHSLSPDKLEVHNGADDNASGTAGLLELAEAMASDPTPRTQGVVFAAFGAEEL
                     GLFGSAWYVDHPVRPIDGLTAMLNMDMIGRLKNGRLEVGGVGTSPPLKGLVEAAWGGT
                     GVTPVYSEGGSGPSDHKTFNDRKRPVLFFFTGVHEDYHRPSDDWEKINSDGMAEVTEG
                     IRKIAMQLAGEAAPLPWTAVAADTTTRLGAVSGSGASLGTIPDYTESDIPGLKLSGVR
                     AGGPAEKAGIQGGDIIVGFDGRVVRNIYDLMEELGSHRPGDTVVVEVLRQRQPLDPAA
                     QPPPEPERLKLTATLERRK"
     gene            3106..3693
                     /locus_tag="VF720_02410"
     CDS             3106..3693
                     /locus_tag="VF720_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878231.1"
                     /translation="MNHRFAGPIMALLGLFATTTCAIAGIPDPALSTIPNVIASPNGT
                     IPYRVTIVGQGGPVADAAVQLRYRAAAEGAACWCTAQGHPIINATTNPSGVATFNVAG
                     GMCLNPAVVAGGVVIEVFVNDIKLKEVGQVSPDVSALPSGSCEVGLSDAVDFTSPLAT
                     NTYGYCFDLNTDGAVGLTDAVIFTNPAATAQTCPE"
     gene            3962..4549
                     /locus_tag="VF720_02415"
     CDS             3962..4549
                     /locus_tag="VF720_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878232.1"
                     /translation="MVRRNRFAGSLAALALLTVGSAAYAGIPDCTLSTIPNVVACPDG
                     SIATTFTIVSTSGPLNGATVELRYTAAGDAAGCWCAVQTHPTISGVTNASGQVTFNVR
                     GGGCLNPALVAGGVAIRVFVNNIACKEIGQVSPDVNSTTSPPCTVSLSDAVDFTGPLS
                     TGTYGFCFDLNSDNQVGLTDAVIFTAPAANAVSCN"
     gene            4671..5000
                     /locus_tag="VF720_02420"
     CDS             4671..5000
                     /locus_tag="VF720_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7878233.1"
                     /translation="MFQDLEITPAELAARRASGQPVRLIDVRESWEFEYNRIEGAELL
                     PLSELEFRHAEFLKPEEFVVCYCHMGMRSFNAAMWLKQQGYADVKSLAGGINRWADEQ
                     DPDMPRY"
     gene            complement(5007..5339)
                     /locus_tag="VF720_02425"
     CDS             complement(5007..5339)
                     /locus_tag="VF720_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7878234.1"
                     /translation="MEIVRWTGAWPASPDRMTKINLFETPRFFLDVYVFEPGQSQKPH
                     VHEVNDKIYLVTEGRGIFQVGAERAELGPGDAVLAPAGVEHGAENTGDARLVALAFMA
                     PHPKSPST"
     gene            5446..>6160
                     /locus_tag="VF720_02430"
     CDS             5446..>6160
                     /locus_tag="VF720_02430"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7878235.1"
                     /translation="MGLYSEDNLYWRKLAHGVAEEVVRPLAAKYDQLQEYPWEIKDAI
                     ARAGLLKVWIPKEYGGAGAGVMNLCIVVEELSRACGGVGVMFAVNALGSFPILVGGTE
                     EQKKEWLPQIATGEKLIAFCLSEKFAGSDAGSLRTAARDEGDYFVLSGEKKWTTNGGA
                     ADIYTVFCVTDPNSRSKRISALIVEKGMEGFSIGKVEDKMGIRCAPVVETVFEDVKVP
                     RRNLLGGTAGHGFRHAMQTL"
BASE COUNT          990 a         2106 c         1991 g         1073 t
ORIGIN      
        1 caccagcatg accggagatg acgccgacgc catccggcgc gaagccccgt cggtggcgcg
       61 cgtggccccc accacgcgca ccgtggcgca gatcgtggcc ggcaaccaga actggagtac
      121 gcaggtgcag ggagccggct ccgactactt cgaagtccgc gactggccgg tcgagtacgg
      181 cctgccgttc acggagctcg atgtccgctc cgccgccaag gtggccatcc tgggcaccac
      241 cgtcgccgag aatctcttcc cgggcgacga cccggtcggc caggtggtcc ggatcaacaa
      301 ggttcccttc tcggtgctcg gcatcctgaa gaagaaaggg gggaacgcga tgggaagcga
      361 ccaggacgat atcatcgtgg ttccctacac cacctcgcag aagcgcctgc aggggatcac
      421 ccacatccag gggatgatga tctcggcggt caccaaggag cgcgtgcccg acgcccagga
      481 cgagatcacc cagatcctcc gccgccgcca caggatcgcc cccggcggca acgacgactt
      541 catcatccgg gcccagtccg acatcgccgc cacggccgag gaaacctcga aagtgatgac
      601 gatgctcctc ggggccatcg catcggtcag cctcctcgtg ggagggatcg gcatcatgaa
      661 catcatgctg gtgtcggtca ccgagcggat ccgcgagatc ggcatccgca tggccgtcgg
      721 tgcccgcccg ggggacattc tcctccagtt cctgctggag gccatcattc tctccacgct
      781 cggcggactg atcggcgtgg gactcggcct cgcgatcagc cagatcgtgt cgaaggccgc
      841 ggggtggtcg gtactggtgg cgccgccggc cgtggcgatg gcattcggct tcgccgtggc
      901 catcggggtc ttcttcggat tctacccggc gcgcaaggcg gccctgctcg acccgatcga
      961 ggcgctgcgg tacgagtagc ggtgccggct acttgcgacg ttcgagcgtg gcggtcagct
     1021 tcaggcgttc cggctcgggc ggaggctgcg ccgcgggatc gagcggttgc cgctggcgca
     1081 gcacttccac caccaccgtg tccccgggac ggtggctccc cagttcttcc atcaggtcgt
     1141 agatgttgcg cacgacccgg ccgtcgaatc ccacgatgat gtcgcctccc tggatcccgg
     1201 ccttctcggc cggaccaccg gcccgcacgc ccgaaagctt gagccccggg atgtccgact
     1261 ccgtgtaatc ggggatggtg ccgaggctgg cgcccgagcc gctcaccgct cccaaccggg
     1321 tcgtcgtgtc ggcggccacc gccgtccatg gcaagggcgc cgcctcgccg gcgagctgca
     1381 tggcgatctt gcggattccc tcggtcacct ccgccattcc atcggagttt atcttctccc
     1441 agtcgtcgga cgggcggtga tagtcctcat gcacgcccgt gaagaagaag agtaccggtc
     1501 tcttccggtc gttgaacgtc ttgtggtcgg agggaccgct tcccccttcg ctgtagacgg
     1561 gggtcacgcc ggtgccgccc caggcggctt ccaccagccc cttgagcggg ggtgaggtgc
     1621 ccacgccccc cacttcgagt cgaccgttct tcaatcgccc gatcatgtcc atgttcagca
     1681 tggcggtgag cccgtcgatg ggacggacgg gatgatccac gtaccaggcc gatccgaaga
     1741 ggccgagctc ctcggccccg aacgcggcaa aaaccacccc ctgcgtgcgc ggcgtcggat
     1801 ccgaggccat cgcctcggcc agctcgagga gaccggccgt accagacgcg ttgtcgtcgg
     1861 cgccgttgtg tacctcgagc ttgtccggcg agagggagtg ctgccccccc atcccgagat
     1921 ggtcgtagtg ggcgccgatg acgaggtgag gttcggacgt cgagtcggcg cggccgacca
     1981 ttcgtgccgc cccaccgttc ggcggcagcc acgccaccac gtttcgcgtg gtcctcgtct
     2041 cggtgcggat gtcgcccttg agcctcacgc tcatactggt gatcggctgc gacagggggc
     2101 gcatctcccc gtcgatccgc ccctggagat ccttgagcga cgccggcttg tcggggagtc
     2161 ccaggcccca gatcgattcg agacccttgc gggtgatcat catggccggg atgccgacgg
     2221 cctgggcggt gccggccttt tcccacgtca gaagatcgtc cgcaccgccc tcgttcagcg
     2281 ggccgttcac gatgatgatg ccggcggcgc catgcgtctc ggcgttccgg gctttcgtgc
     2341 ggaggttcgc gtgccaggtg tccttgcgcc cctcgaagat cgagcctgtg tcatcgcggg
     2401 ccggctcgta ccggaggcag acgacgactt ttccggagac gtccaggccc gcgtagtcgt
     2461 cgtagccgtg ctcggcggcg gtgatgccgt acccgacgaa gatggccggc gccgcgcact
     2521 cgccgctggc ggaaaaggcc aacggaacga attcctttcc gagctccagg tcgtgcggaa
     2581 gcttcttcgt ggggagcgtg acggcgtttc cgtcgccgag cgaactggcc accttcgccg
     2641 gaaattcctg gtaccagccg ccgttgtcgc cggcgggctg gaccccggcc gccttgaagg
     2701 catcggcaat gtaacgagcc gccatttcgc cccccgcctc gccggtgccc cggccggcaa
     2761 attcgtcgga ggccagggtg cggacatgcc cgctcagttc gccggcggtg atggggtcgg
     2821 cgggcgcggc atgcgcgggc ctcacgggca aggagctggg tccggccgcc aggaacaggg
     2881 ccgccatcgc ggccggtggg atcaagcgtc gattgcgcat gtcggtatcg gctctccggt
     2941 catgacaggg ggcggggagg tcgcgaagtg tatcagggct cccgcccccg cggcgcctcc
     3001 ccattcttat cccttccgcc aaccctccga cgcagggccg ccacatgaac cggatagctt
     3061 cctccgtgcc aaccaggacc acgaaccgtc tacggaggtg ccaccatgaa tcatcgcttc
     3121 gcgggcccga tcatggccct gctcgggctg ttcgccacca cgacctgtgc cattgccggt
     3181 attccggacc cggcgctcag cacgatcccc aacgtgatcg cctcgcccaa cggcacgatc
     3241 ccgtaccggg tgaccatcgt ggggcaggga gggccagtgg ccgacgccgc ggtccagctc
     3301 cgctaccgag ccgcggccga aggggcggcc tgctggtgca ccgcccaggg ccatccgatc
     3361 atcaatgcca caaccaaccc gtctggggtc gccaccttca acgtggctgg cggcatgtgc
     3421 ctgaaccccg cggtggtggc cggaggcgtg gtcatcgagg tcttcgtcaa cgacatcaag
     3481 ttgaaggaag tcggccaggt gagcccggat gtctccgcgc tcccgtccgg atcgtgcgag
     3541 gtgggtcttt cggatgccgt ggatttcacg tcgccgctgg ccaccaacac ctacggctat
     3601 tgcttcgatc tcaatacaga cggggccgtg ggcctgaccg atgccgtcat ctttaccaat
     3661 cccgccgcca ccgcccagac gtgccccgag tgaaccctca ttggtaacgc cttgatgtga
     3721 aacgatatat gcggcggcta cctcccggag ggggccgccg cccccgtttt tgggcggaag
     3781 tccgccgttc gtttcgctgt cattccgcgt atgaataggc tagacttctc agccgaagca
     3841 attgcttcga cttccagcgg tctcgcgagc ggctggtccc ccggtcccgg tgaaccggta
     3901 tccatctcca gccagtttca gttcaaccca gatttacggc atcgttccgt ggaggtgtca
     3961 catggttcgt cgtaaccgtt tcgccgggtc gttggcggcg cttgctctcc tcacggtcgg
     4021 gtcggcggcc tacgccggaa tccccgactg tacgctcagc accattccca acgtcgtggc
     4081 ctgcccggac gggtcgattg ccaccacgtt cacgattgtc agcacctcgg gtcccctgaa
     4141 cggggccacg gtggaactgc gctataccgc cgccggcgac gctgccggct gctggtgcgc
     4201 ggtccagact cacccgacca tttccggtgt cacgaatgcg tcgggccagg tcacgttcaa
     4261 cgtccgcggc ggtgggtgcc tgaatcccgc actcgtggcg ggcggcgtgg ccatccgcgt
     4321 attcgtcaac aacatcgcct gcaaggaaat cggtcaggtc agcccggacg tcaacagcac
     4381 cacgtcgcct ccgtgcaccg tcagcctctc ggatgccgtc gatttcacgg ggccgctttc
     4441 cacgggcacc tacggcttct gcttcgatct caattccgac aaccaggtcg gccttacgga
     4501 cgcggtcatc ttcaccgccc cggctgccaa cgcggtgagc tgcaactagc aggatcttga
     4561 gggcgcagca ccacggacag acatttcgag cgcggcgaat caggcgatcc gccgcgcttt
     4621 tctttgcggc gcgaagaccg cgcatgggcg ccggagcccc ggaggaaagc atgttccagg
     4681 acctggaaat cacccccgcg gaactggccg cacgccgcgc gtccgggcag cccgttcgcc
     4741 tcatcgatgt ccgcgagtcg tgggagttcg agtacaaccg aatcgagggg gccgagctgc
     4801 tgccgttgtc ggagctcgaa ttccggcacg cggagttcct caagccggaa gagttcgtcg
     4861 tctgctactg ccacatgggg atgcgcagct tcaacgccgc catgtggctg aagcagcagg
     4921 ggtatgccga cgtgaagagc ctggccggcg gcatcaaccg ctgggccgac gagcaggacc
     4981 cggacatgcc gcgctactga tcgacttcat gtggacggcg atttgggatg tggcgccatg
     5041 aaggccaggg ccaccagccg ggcatctccg gtgttttccg cgccgtgctc cacgccggcc
     5101 ggcgcgagga cggcgtcacc cggccccagt tccgcccgtt ccgcgcccac ctggaagatt
     5161 ccgcgcccct ccgtcaccag gtagatcttg tcgtttacct cgtgtacgtg gggcttctgg
     5221 gactgccccg gctcgaaaac gtacacgtcc aggaagaagc ggggcgtctc gaagagattt
     5281 attttcgtca tccggtcggg cgaggccggc caggctccgg tccagcgaac gatctccatg
     5341 cgtgctcccc cgggcggtcg gccgggacgt tccgtgcgcc cgaaccgtgt gataacctcg
     5401 cggacttgcg cgttgcgaac ggcatcttat cccaccggag gaatcatggg cctgtacagc
     5461 gaagacaacc tctactggcg aaagctcgcc cacggcgtgg cggaggaagt cgtccggccc
     5521 ctggcggcca agtacgacca gctgcaggag tatccgtggg agatcaagga cgccatcgcc
     5581 agggccggcc tcctcaaggt gtggatcccg aaggaatacg gcggcgccgg ggccggggtc
     5641 atgaacctct gcatcgtggt cgaggaactc tcgcgggcct gcggcggtgt cggcgtcatg
     5701 ttcgcggtca atgcgctcgg ctcgttcccg atcctggtcg gcggcaccga ggagcagaag
     5761 aaggagtggc tgccccagat cgccaccggt gagaagctca ttgccttctg cctctccgaa
     5821 aaattcgccg gctcggacgc cggctcactc cgcaccgccg cccgcgacga gggggactac
     5881 ttcgtcctct ccggtgagaa aaagtggacg accaacggcg gcgcggccga catctacacg
     5941 gtcttctgcg tcaccgaccc caattcccgc tccaagcgca tctcggccct catcgtcgag
     6001 aagggaatgg aaggattctc gatcggcaag gtcgaggaca agatggggat ccgctgcgcc
     6061 ccggtcgtcg aaaccgtctt cgaggatgtg aaggtaccgc gaaggaacct gctcggcgga
     6121 acagccggcc acggcttccg ccacgccatg cagacgctcg
//
LOCUS       DASVOD010000124         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_321351, whole genome shotgun sequence.
ACCESSION   DASVOD010000124 DASVOD010000000
VERSION     DASVOD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321351"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..915
                     /locus_tag="VF720_02435"
     CDS             121..915
                     /locus_tag="VF720_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEX7878236.1"
                     /translation="MRILIIADIVGNPGRKAVRAFVPKLKAEHGIDFVIANGENAAGG
                     IGMTKETSDDIFASGVDVMTSGNHVYDKPEMMDYLPREPRIVRAANFPNGAPGSPLGL
                     YPTPKGTVALLTVLGRTFMKPLEDPFNVARKKILEAREQGAKIVVVDFHAEATSEKVA
                     LGWYLDGLASVVIGTHTHVPTADERVLPGGTAYCTDIGMTGPFDSVIGVEKQAAIHRF
                     ITGLPVKFKPAGKDVRLCGVIVDCDETTGKGTAIRRVMEYLPDQVG"
     gene            952..>1608
                     /locus_tag="VF720_02440"
     CDS             952..>1608
                     /locus_tag="VF720_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HEX7878237.1"
                     /translation="MSATLLPGAPVAEALLAETALEVERLRSFGITPALALVRVGDDP
                     ASVVYLRRKAEACAKVGIASIDRVFDAETSERTVLEAIEELNADRAVHGILVQLPLPT
                     HLSEALILRAVDPDKDVDGFHPLNAGRLVAGLPGFVPCTPFGIIRMIRHAGIDLAGKH
                     AVVVGRSNIVGRPMANLLSRKAPGLNATVTLAHAASGDLARYLRDADLVVAAVGRPGT
                     V"
BASE COUNT          268 a          561 c          540 g          239 t
ORIGIN      
        1 ggcggccgag atcgcccgga agatcgaagg ggagctgcag taccccggac agatcaaggt
       61 cgtcgtcatc cgggaaaccc gcgccgtcga cttcgccaag tagatggaaa cgtcctccag
      121 gtgagaatcc tcatcatcgc cgacatcgtc ggcaatccgg gccgtaaggc ggtgcgcgcc
      181 ttcgtgccga agctcaaggc cgagcacggc atcgatttcg tcatcgccaa cggcgagaac
      241 gcggccggcg gcatcggcat gaccaaggaa acatccgacg acatcttcgc ctcgggcgtg
      301 gatgtcatga ccagcggcaa ccatgtgtac gacaagcccg agatgatgga ctacctgccg
      361 cgcgaaccgc gcatcgttcg ggcggccaat ttccccaacg gcgcgccggg ctcaccgctc
      421 ggcctgtacc ccacgccgaa gggaacggtg gcgctcctca cggtgctcgg ccggacgttc
      481 atgaagccgc tcgaagaccc gttcaacgtg gcccgcaaga aaatcctcga ggcccgcgag
      541 cagggggcga agatcgtggt ggtcgatttc cacgccgaag ccaccagcga gaaggtggcg
      601 ctcggctggt acctcgacgg cctggcctcg gtggtcatcg gcacgcacac gcatgtgccg
      661 accgcggatg aacgcgtcct gccgggcggc acggcttact gcaccgacat cgggatgacg
      721 gggccgttcg actcggtgat cggcgtcgag aagcaggccg ccatccaccg cttcatcacg
      781 ggcctgccgg tcaagttcaa gccggcgggg aaggacgttc gcctgtgcgg ggtcatcgtc
      841 gactgcgacg agacgaccgg caagggcacg gccatccgac gcgtcatgga gtatctgccg
      901 gaccaggtgg ggtgaggcgg cgatgatcgc gcccaaccgg actcatcggc cgtgagcgcc
      961 acgctcctcc ccggcgctcc ggtggccgag gcgctcctcg ccgaaaccgc cctcgaggtc
     1021 gagcgcctcc gctcgttcgg catcacaccg gccctcgccc tcgtccgtgt cggcgacgac
     1081 ccggcctcgg tcgtgtacct ccgccggaag gccgaggcct gcgcgaaggt gggcatcgcc
     1141 tccatcgacc gggtcttcga cgcggagacc tccgagcgaa ccgtcctcga ggccatcgag
     1201 gagctgaacg ccgaccgggc ggtccacggc atcctcgtgc agctgccgct gccgacgcat
     1261 ctgtccgagg cgttgatcct gcgcgccgtc gatccggaca aggacgtcga tggttttcac
     1321 ccgctcaacg cggggcggct ggtggccggc cttccggggt tcgtgccgtg cacgccgttc
     1381 ggcatcatcc ggatgatccg ccacgccggc atcgacctgg ccggcaagca cgcagtggtg
     1441 gtgggacgca gcaacatcgt cgggcggccc atggccaatc tcctgtcccg gaaggcgccg
     1501 ggtctcaatg ccaccgtcac gctcgcgcac gcggccagcg gcgatctggc ccgttacctg
     1561 cgcgacgcgg acctcgtcgt ggccgccgtg gggcggccgg gcaccgtc
//
LOCUS       DASVOD010000125         8004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_634241, whole genome shotgun sequence.
ACCESSION   DASVOD010000125 DASVOD010000000
VERSION     DASVOD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8004
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634241"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1191)
                     /gene="bshA"
                     /locus_tag="VF720_02445"
     CDS             complement(67..1191)
                     /gene="bshA"
                     /locus_tag="VF720_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEX7878238.1"
                     /translation="MKIGITCYPSAGGSGVMATELGKRLAGLGHEVHFITSALPVRLR
                     GFDENIYFHEVIPDAYPLFQYPPYEVSLATKMHDVALHHGLDVLHVHYAIPHAVSSFL
                     AREMLKPRKIATVTTLHGTDITLVGVLPSFHRLTRFSIECSDGVTAVSEWLRRQTEEK
                     FPMERRVEVIPNFIDTKIFRPVENPRARCRLGGADEKIVMHISNFRPVKNIEAVVATF
                     AGIAARVKSRLVLIGDGPELARADAIARDFGIYDRVNFLGNQEFIEELLPAADLFLLP
                     SHHESFGLVALEAMSAGVPVIATNQGGPVEVIEDGVTGYLRAPDDVPGMVSAALEVLG
                     DRDRAIHMGEAARRVAVDTYDTDRVVPRYVDFYRATLDSV"
     gene            complement(1188..1919)
                     /gene="bshB1"
                     /locus_tag="VF720_02450"
     CDS             complement(1188..1919)
                     /gene="bshB1"
                     /locus_tag="VF720_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX7878239.1"
                     /translation="MSPSDRLDVLAFAPHPDDLELCCGGTLLRLIDAGHRVGLADLTR
                     GDRGTRGTPEIRMAEAEAARLAMGAHRRINLGFPDLGIDARDRDQLRHVVEAIRRHRP
                     RLVLTCSEHDHHPDHVEAAHLVERGVYVAGLAGFEADGEPFRPARLLFYMGRVVFEPK
                     LIVDVTSVWERRMAVAACFRSQFFREPDDPRVTPISEPGFMADVAARFRHFGSRIGVT
                     YAEPFDLREPFGLTEVGPLLAGGMP"
     gene            complement(1906..3516)
                     /gene="bshC"
                     /locus_tag="VF720_02455"
     CDS             complement(1906..3516)
                     /gene="bshC"
                     /locus_tag="VF720_02455"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HEX7878240.1"
                     /translation="MRLPLTAVPGYGAFFRDWAAGKLPAELDTLRRPERAADRTRVME
                     ERPVFPVSDELAATLTVTHTRWHAPEASLANVAHLAAGKALVVLTGQQPGLLGGPLYT
                     RLKIGTAVAMAEALQRSLGRPVVPLYWNAADDADFDEVAHGTLARNDLKLLRFSLPAA
                     ARVPRGWVGDLPGSRVIEALAPGVGAGLGESFDAWHAGLTARAEGLDFGELHAAEALR
                     WYGSRGLVVVDARWPELRRASARLFTNYLEASARVRDAVVSAGDAQRRAGYEPPLGTE
                     AAAGALFLTPERTRLKLEPAEALAEARRLVSTHPSHLSPNVVLRPLVNDVVFPTIAHV
                     AGPAEVQYLTQLVPVYDALGVCRPLVLDRALATLVPAVALPVAGELARLDGGAAADGL
                     ATLLADPVEGLRRWFGGRMPAGLAGRIETARADLARVFEELREPSHALDASLSQILDS
                     AREKALFQFERFPEGAYKKVRQREETARPGLAGLAEFLKPRTRLQERELSALAVELAG
                     AESAVDQAVAEHVAGLERGERGHHLVTI"
     gene            complement(3513..4280)
                     /locus_tag="VF720_02460"
     CDS             complement(3513..4280)
                     /locus_tag="VF720_02460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7878241.1"
                     /translation="MTRAPMVEPAGRSPWFETAFGPLYSEIYQHRDEDEAELAVAMML
                     SRGVLQGPVLDIGCGTGRHLVPLQKEGVPGIGLDLSSHLLEEAATRAPGVLVRGDMRQ
                     LPFREASFGSALSMFTTFGYFADAAENRRVLKEAARVVRPDGFLAIDYFNAVRTVADL
                     APESTRFVGRYDVIERRTVARDERGERLVKTIDVFEGSKRVDQLREEVRIYQPAELAE
                     AIASAGWTELVRLGDYQGHDFDEAVSPRLIILARRGG"
     gene            complement(4277..5074)
                     /locus_tag="VF720_02465"
     CDS             complement(4277..5074)
                     /locus_tag="VF720_02465"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX7878242.1"
                     /translation="MSLAGVRHVVVVSSGKGGVGKSTVTTNLAVCLARSGKSVGVLDA
                     DVYGPDIPIMFGVSQPPTIANNRIQPPEEHGVKLMSLGFLVDPEQAVIWRGPMVMQAL
                     DQMMNQVDWGHLDILLVDMPPGTGDAQLTMTQKVKLSGAILVSTPQAVALADTIKGLA
                     MFRKVETPILGMVENMSEFICPHCGGTSEFFRRGTVLEACRHYEVPYLGDIPFDPACR
                     EGADRGIPITVSQPGSPVGQAFRAVADRILEAMPEPALPIPIVRRAE"
     gene            complement(5071..5400)
                     /gene="trxA"
                     /locus_tag="VF720_02470"
     CDS             complement(5071..5400)
                     /gene="trxA"
                     /locus_tag="VF720_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7878243.1"
                     /translation="MPVKAQEVTEATFDSEVLKSSIPVMVDFWAVWCGPCKMIGPIIE
                     DLAAAYDGQVKVVKVDIDSNQNLATKYSIRSIPTILFFKNGNVVDQVVGASPRAEFER
                     RLKTVIG"
     gene            complement(5434..5886)
                     /locus_tag="VF720_02475"
     CDS             complement(5434..5886)
                     /locus_tag="VF720_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878244.1"
                     /translation="MFRSIRKFGAVLALLVTGCAGGRSAIPLNESTSLPDKDVYYVTT
                     TMGEQLDFVTLESDGAVISGTVREVRERLVGQGDDERVESRSQYRDVRMPLSDVARIE
                     VDKPGSKPLVLIAAGAAAIGGAFLIFSNGGDDATTGGGGGGKGPPDLP"
     gene            complement(6181..>8004)
                     /gene="mutS"
                     /locus_tag="VF720_02480"
     CDS             complement(6181..>8004)
                     /gene="mutS"
                     /locus_tag="VF720_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEX7878245.1"
                     /translation="RRTMFLDEATRRNLEILKPAFEGPDTSLLGVIDRTRTAPGGRRL
                     RAWLEQPLLDRGAIEARHDAIDDFHARAEEATELADLLRDTHDLERLLGRAACGKAGP
                     RDLHALGTTAVRTRDFRRVAGTFTAPRLAELAEEIADLEPLGRRLLDALVDEPPATAA
                     DGGVFRDGYHAELDQLRRGNVSGTEWIAALQARERERTGITSLKVGFNKVFGYYLEIT
                     NTHKHLVPDDYIRKQTLVNAERYVTPDLKEEEARVLGAEDRIRKLEGELFRALRDEVV
                     AESLRVRRTAAALAELDALLALSVVARERSFVRPRMVDGPVLLVRDGRHPVVDRLLPA
                     GQFVPNDIDMDGAKRQVLLITGPNMAGKSTYLRQTALIVLLAQTGSFVPATEATVGIV
                     DRIFTRIGASDSIARGRSTFMVEMQETAAILRNATDRSLVVLDEVGRGTSTFDGLSIA
                     WAVTEHLHDGPSVKPRTLFATHYHELTHLARDLDRVVNLNVQVREWNDQVHFLHKIVP
                     GAADRSYGIQVARLAGLPDPVLARAREVLELLEAGRLDKVEESRHEEARPQLQLFDPP
                     GAAVLEELAALEPEKMTPLEALNQLDRLHRLHRNGKSPRRR"
BASE COUNT         1287 a         2707 c         2777 g         1233 t
ORIGIN      
        1 ctggcattcg catcgcggct cgtccggcgg cgccggtcgg cttaccgcct ggatcgttcg
       61 cccggatcag accgaatcga gcgtggcccg gtagaagtcg acgtaacgag gcaccacgcg
      121 atcggtgtcg tacgtatcca ccgccacccg gcgggcggcc tctcccatgt ggatcgcgcg
      181 gtcgcgatcg cccaggactt cgagcgcggc actcaccatg ccggggacat cgtcgggcgc
      241 gcgcaggtag ccggtgacgc catcctcgat cacctccacc ggtcccccct ggttcgtggc
      301 gatgacgggg acgccggcac tcatcgcctc gagcgccacc aggccgaagc tctcgtggtg
      361 gctcggcaac aggaagaggt cggccgccgg caggagttct tcgatgaatt cctgattgcc
      421 gaggaaattg acccggtcgt aaatgccgaa atcccgggcg atggcgtcgg cccgggccag
      481 ctcgggacca tcgccgatca gcacgagccg cgacttcacc cgggccgcga tgccggcgaa
      541 ggtggccacg accgcctcga tgttcttgac cggccggaaa ttcgagatgt gcatcacgat
      601 cttctcgtcg gcgccgccga gccggcagcg ggcgcggggg ttctcgaccg gccggaagat
      661 cttcgtgtcg atgaagttcg ggatgacctc gacgcgccgt tccatcggga acttctcctc
      721 cgtctggcga cgcaaccact cggagacggc agtcacgccg tccgagcact cgatcgagaa
      781 gcgcgtgagc cggtggaagg acggcaggac gccgaccagc gtgatgtcgg tgccatgcag
      841 cgtcgtcacg gtggcgatct tgcggggctt cagcatctcc cgggccagga aggacgacac
      901 ggcatgcggg atggcgtagt gcacgtgcag gacatcgagc ccgtggtgga gtgccacgtc
      961 gtgcatcttc gtggccagcg agacctcgta cggcgggtac tggaagagcg gataggcatc
     1021 gggaatgact tcgtggaaat agatgttctc atcgaagccg cgcaggcgga ccggcaacgc
     1081 ggaggtgatg aagtgcacct cgtggccgag cccggccagg cgcttgccca gctcggtggc
     1141 catgacgccc gaaccgccgg ccgaggggta acaggtgatg ccgatcttca cggcatccct
     1201 cccgccagca gcggcccgac ctcggtcagt ccgaacggct cgcgcaggtc gaacggctcg
     1261 gcgtacgtga cgccgatccg gcttccgaag tggcggaagc gcgcggccac gtcggccatg
     1321 aagccgggct ccgaaatggg cgtcaccctg gggtcgtcgg gctcgcggaa gaactggctc
     1381 cggaagcagg ccgccacggc catgcggcgt tcccacaccg acgtcacgtc cacgatcagc
     1441 ttgggttcga acaccacgcg ccccatgtag aagaggaggc gcgccggccg gaacggttcc
     1501 ccatcggcct cgaagccggc caggccggcc acgtacacgc cgcgctccac caggtgggcc
     1561 gcctccacgt ggtccgggtg gtgatcgtgc tcgctgcagg tgaggacgag gcgcggccga
     1621 tgtcgtcgga tggcttccac gacgtggcgg agctggtccc ggtcgcgtgc gtcgatgccg
     1681 aggtcgggaa aaccgagatt gatccgccgg tgcgctccca tggccaggcg ggccgcctcg
     1741 gcctcggcca tccggatttc cggcgtgccg cgcgtgccac gatcgccgcg cgtcaggtcg
     1801 gcgaggccca cccggtggcc cgcgtcgatg agccggagca gcgtgccgcc gcagcagagt
     1861 tcgagatcgt cgggatgggg cgcgaacgcc aggacatcca gccggtcaga tggtgacaag
     1921 gtggtgcccc ctctcccctc tctcgaggcc ggccacatgt tcggccaccg cctggtcgac
     1981 ggcgctctcc gcgccggcca gttccaccgc gagcgccgag agctcccttt cctgtaaccg
     2041 cgtgcgcggc ttgaggaatt ccgccaggcc ggccaggccc ggccgggccg tctcctcccg
     2101 ctggcgcacc ttcttgtacg ctccttcggg aaatctttca aactggaaga gcgccttctc
     2161 gcgcgccgag tcgaggatct gcgagaggct cgcgtcgagc gcgtgtgacg gctcacgcag
     2221 ttcctcgaag acgcgggcaa ggtcggcccg ggccgtctcg atccgcccgg cgagcccggc
     2281 cggcatgcgc cccccgaacc agcggcggag cccctcgacg gggtcggcga gcaatgtggc
     2341 aagcccgtca gcggccgcgc ccccatccag ccgtgccagt tctcccgcaa ccggcaatgc
     2401 caccgccggc accagcgtgg ccagcgcccg gtcgagcacc agggggcggc agacgccgag
     2461 cgcgtcgtac accgggacga gctgcgtcag gtactgcacc tcggccggac cggccacatg
     2521 ggcgatcgtc gggaagacca cgtcgttgac cagcggccgg agcaccacgt tgggtgagag
     2581 gtgggaggga tgcgtcgaca cgaggcgccg ggcttccgcc agggcctcgg ctggttcgag
     2641 cttgaggcgc gttcgttccg gcgtgaggaa gagcgctccg gcggccgcct cggtgccgag
     2701 ggggggctcg tagccggccc ggcgctgggc atcaccggcg gagaccaccg cgtcgcgcac
     2761 gcgcgccgac gcttccaggt aattcgtgaa gagtcgggcg gaggcgcgac gcagctcggg
     2821 ccagcgggca tccaccacga ccagcccgcg cgatccgtac cagcggagcg cctcggcggc
     2881 gtgcagctcc ccgaagtcga gcccctcggc acgggcggtg agaccggcgt gccaggcatc
     2941 gaacgactca ccgagcccgg ctccgactcc cggcgccagg gcctcgatca ccctgcttcc
     3001 cggaagatcg cctacccagc cgcgcggcac gcgcgccgcc gccggcaagg aaaatcggag
     3061 cagtttgaga tcgttgcgcg ccagtgtccc gtgcgccact tcatcgaagt cggcgtcgtc
     3121 ggccgcgttc caatagaggg gcacaaccgg gcgaccgagc gaacgctgga gcgcctcggc
     3181 catggccacc gccgtcccga tcttgaggcg cgtataaagc ggccccccca ggagtcccgg
     3241 ctgctggccg gtcaggacca ccagcgcctt gccggcggcg agatgtgcca cgttggcaag
     3301 ggacgcctcc ggcgcgtgcc atcgcgtatg ggtaacggtg agcgtggcgg ccagctcgtc
     3361 gctcaccgga aacaccggcc gttcctccat cacccgagtc cggtcggccg cccgctcggg
     3421 gcggcggagc gtatcgagtt cggccggcaa ctttccggcg gcccagtcgc gaaagaaagc
     3481 gccgtagccg gggacggccg tcaacgggag cctcaaccgc ccctccgcgc caggatgatg
     3541 agacgtggcg acaccgcctc gtcgaagtcg tgcccctggt agtcgccgag tcgcacgagt
     3601 tcggtccaac cggcggaggc aatggcctcg gccagctcgg ccggctggta gatgcgcacc
     3661 tcctcacgga gctggtcgac ccgcttcgag ccctcgaaaa cgtcgatggt cttgaccagc
     3721 ctctcccccc gctcgtcacg cgccacggtg cgccgctcga tcacgtcgta gcgcccgacg
     3781 aaccgtgtgc tctcgggggc caggtcggcc acggtgcgga cggcgttgaa atagtcgatg
     3841 gccaggaagc cgtcagggcg gacgacccgg gccgcctcct tcagcacgcg ccgattttcg
     3901 gcggcatcgg caaagtagcc gaacgtggtg aacatgctga gcgccgagcc gaacgacgcc
     3961 tcgcggaacg ggagctggcg catgtcgccg cgtaccagca cgccgggcgc ccgggtggcc
     4021 gcctcttcga gaagatgact cgaaaggtcg aggccgatac cgggcacccc ctccttctgg
     4081 aggggtacga ggtgacgccc ggttccgcaa ccgatgtcga gcaccgggcc ctgcagcacc
     4141 cctcgcgaca gcatcatcgc caccgccagt tcggcctcgt cctcatcgcg atgctggtat
     4201 atctccgaat agagcggtcc gaaagcggtt tcgaaccagg gggagcggcc ggccggctcg
     4261 accatcgggg cccgggtcac tccgctcgcc ggacgatcgg gatcggaagg gcgggttcgg
     4321 gcatggcctc gaggatccgg tcggccacgg cacggaaggc ctgccccacc gggctccccg
     4381 gctggctcac cgtgatgggg atgccgcggt cggcgccctc ccggcaggcc ggatcgaatg
     4441 ggatgtcgcc gagatacggc acctcgtagt gccggcatgc ctcgaggacc gtgccgcgcc
     4501 ggaagaattc ggacgtgccg ccgcagtggg ggcagatgaa ctcgctcatg ttctccacca
     4561 ttccgaggat cggcgtttcc accttgcgaa acatggccag ccccttgatg gtgtcggcca
     4621 gcgccaccgc ctgaggcgtg gacacgagga tggcgcccga gagcttcacc ttctgcgtca
     4681 tcgtgagctg ggcatccccg gtgccggggg gcatgtcgac gagcaggatg tcgagatggc
     4741 cccagtcgac ctggttcatc atctggtcga gggcctgcat gaccatgggc ccccgccaga
     4801 tcacggcctg ttccggatcg accaggaagc cgagcgacat cagcttgacg ccgtgctctt
     4861 ccggcggctg gatccggtta ttggcgatgg tgggaggttg cgacaccccg aacatgatcg
     4921 ggatgtccgg gccgtagacg tccgcgtcga gcacccccac cgacttcccg gaacgcgcca
     4981 ggcagacggc caggttggtc gtgaccgtgg acttccctac cccgcccttg ccgctcgaca
     5041 ccacgactac gtggcgtacg ccggccaggc tcatccaatg accgtcttga gtcgccgttc
     5101 gaactcggcc cgcggggaag ccccgacgac ctggtcgacg acgttgccat tcttgaagaa
     5161 gagaatcgtc gggatgctgc ggatggaata cttcgtggcg aggttctggt tgctgtcgat
     5221 gtcgaccttg accaccttga cctggccatc gtacgcggcg gccaggtcct cgatgatcgg
     5281 cccgatcatc ttgcaggggc cgcaccagac ggcccagaag tcgaccatga cggggatcga
     5341 cgatttgagc acttcactgt cgaacgtggc ttcggtgact tcctgggctt tgacgggcat
     5401 gtattcctcg ggtaaagggg ggattggaaa acgtcagggt agatcgggcg gtcccttgcc
     5461 gccgccgccg ccgccggtgg tggcatcgtc gccgccgttc gagaagatca ggaacgcgcc
     5521 accaatggcg gccgcgccgg cggcgatcag gacgagtggt ttcgagccgg gcttgtcgac
     5581 ctcgatgcgt gcgacgtcgg agagcggcat gcggacatcg cggtactggc tgcgggattc
     5641 cacgcgctca tcgtccccct gccccacgag acgctcgcga acctcgcgga cggtgccgga
     5701 aatgaccgcg ccatcggact cgagggtgac gaaatcgagc tgttccccca tcgtggtcgt
     5761 gacgtagtag acgtctttgt ccgggagcga ggtggattcg ttcaacggaa tcgcggaacg
     5821 tccaccggca caaccggtta cgagcagcgc caggacggcg ccgaattttc ggatggatcg
     5881 gaacatggaa tcacctcggc aagcccaacg gcttgagact agcaaacatg cgaggggatt
     5941 caaaccgctg gagagggcgg ttcggcggcc ggaggggccc cgggccggta aaaaaatcgt
     6001 gaggcgtcgc gttcgacgga ctgacccgtg acgaaaacct cagtgcggcc ggcggttcgt
     6061 cgctaaggag gcaaaacccg aaacgccggc tgtcggtgcc cagcgaaggt tcaccacgga
     6121 cagctccgtg aagcgctgac gcctcactgt aattcaatat aggaacgaat gagccggtcg
     6181 tcaacgtcgc cggggtgact ttccatttcg atggagccgg tgcagccgat cgagctggtt
     6241 gagcgcctcg aggggcgtca ttttttccgg ttcgagtgcc gccagctcct cgaggaccgc
     6301 ggcacccggg ggatcgaaca gctggagctg cgggcgcgct tcttcgtgac gactctcctc
     6361 caccttgtcg agccggccgg cctccaggag ctccaggacc tcccgggccc gcgcgaggac
     6421 cggatcgggc aaaccggcca accgcgccac ctggattccg tacgaccggt ccgcggcgcc
     6481 cggcacgatc ttgtggagga agtgcacctg gtcgttccac tcgcgcacct gcacattcag
     6541 gttcaccacc cggtcgaggt cgcgggccag gtgcgtgagc tcgtggtagt gcgtggcgaa
     6601 gagcgtgcgc ggctttaccg acggaccgtc gtgcaggtgt tcggtgacgg cccaggcgat
     6661 cgagaggccg tcgaacgtcg acgtccccct cccgacctcg tcgagcacca ccaggctccg
     6721 gtcggtggcg ttgcggagga tggcggccgt ctcctgcatc tccaccatga aagtggagcg
     6781 cccgcgggcg atcgaatcgc tggcgccgat gcgggtgaag atccggtcga cgatcccgac
     6841 ggtggcctcg gtggccggca cgaaggaacc ggtctgggcc agcagcacga tgagggccgt
     6901 ctggcggaga tacgtggact tgccggccat gttgggaccg gtgatgagga ggacctgccg
     6961 cttcgccccg tccatgtcga tgtcgttggg cacgaactgg cctgccggca ggagccggtc
     7021 gacaaccggg tggcggccgt cccggacgag gagcaccggg ccatcgacca tgcgcgggcg
     7081 gacgaaagag cgctcccggg ccaccaccga gagggccagc agggcatcca gctcggccag
     7141 ggccgccgcg gtccggcgga cgcggagcga ttcggccacc acctcgtcgc gcaacgcccg
     7201 gaagagctct ccttcgagct tccggatccg gtcctcggcg ccgaggacgc gggcctcctc
     7261 ttccttcagg tcgggcgtca cgtagcgttc ggcattcacc agcgtctgct tgcggatgta
     7321 gtcgtccggc acaaggtgct tgtgggtgtt ggtgatctcc aggtagtagc cgaacacctt
     7381 gttgaagccg accttgagcg acgtgatgcc ggtccgctcc cgctcgcggg cctggagcgc
     7441 cgcgatccac tccgtccccg acacgttccc ccgccggagc tgatcgagct cggcgtggta
     7501 cccgtcccgg aagacgccgc cgtcagccgc ggtggcgggt ggctcgtcca cgagtgcgtc
     7561 gagcaggcgg cggcccaggg gttcgaggtc cgcgatttcc tcggcgagtt ccgcgagtcg
     7621 cggcgcggtg aacgtgcccg ccacccggcg gaagtcacgc gtgcggacgg ccgtagtccc
     7681 cagcgcgtgc aggtcgcggg gcccggcctt gccgcaggcc gcccggccga gaagccgctc
     7741 caggtcgtgc gtatccctca acaggtcggc cagctcggtg gcctcctcgg cccgggcatg
     7801 aaagtcgtcg atcgcatcgt gtcgcgcctc gatggccccc cgatcgagga gcggttgctc
     7861 aagccaggcg cgcaggcgcc ggccgccggg cgccgtccgc gtgcggtcga tgacgccgag
     7921 aagcgaagtg tcgggccctt cgaacgccgg cttcaggatc tcgaggttcc ggcgcgtggc
     7981 ttcgtcgagg aacatcgtcc tccg
//
LOCUS       DASVOD010000126        14620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_165762, whole genome shotgun sequence.
ACCESSION   DASVOD010000126 DASVOD010000000
VERSION     DASVOD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14620
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165762"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VF720_02485"
     CDS             <1..301
                     /locus_tag="VF720_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019393577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7878246.1"
                     /translation="GLSNDPALIDWVAGVLTPVVGPDAVIHLPKPIMGGEDFAHYLEV
                     MTGAFLRIGVRDESRGIVHPLHSPRFDLDEAALPFGAGVLATVARAWLIRAAEAR"
     gene            complement(326..829)
                     /locus_tag="VF720_02490"
     CDS             complement(326..829)
                     /locus_tag="VF720_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878247.1"
                     /translation="MSDNNTKVGSVVVVITTDVTQSTPQARVRNMTLTAYDENGNPIV
                     GESWGNFQEANLPKVKNKATTTFVWNDVNQAIRVNQFWLTYTAQPGTSNASPFDNLGS
                     GTGSWVFNNTGVQGGSLANGTDGNALAISNKGSWEYAISLDLVDPGSGTHYSCQHDPK
                     MDVGDIG"
     gene            complement(859..3111)
                     /locus_tag="VF720_02495"
     CDS             complement(859..3111)
                     /locus_tag="VF720_02495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878248.1"
                     /translation="MIGRTLLRYQVVARLGQGGMGVVYQGHDPRLDRPVALKVLPPDR
                     LADPIERRRFEREARVASSLNHPGIVTIYEIGTADGVDVIAMEYITGRQLAELIPAGG
                     LPIGKALDWGAQMADALAKAHAAGIIHRDLKPANVMVNEDGRIKILDFGLARAVEMRP
                     APGAEAGASAVGADDATLTAPLTMAGTVSGTIAYMSPEQAAGETLDARTDVFSFGIVL
                     YQMLSGINPFRGDTAVNTLQRLLVAEPPPLASHRGEMPAGLDSLVRNCLRRDRDDRRL
                     AMAGIAAALRSIERELPLTDAPTVTMAPAVSPAPLGSMTDATLPARRTGRDPLILAAV
                     LLAAIVGGGLAYQALIRTKQPVTAAPDGPKGSAELVTEARRRLDRYDRPKNVEESIVL
                     FHSAIAADSNSAPAWAGLAEACHQKFLAAKDEHWLRQAEAAAAEALRRNDQLALSHIA
                     MATVELQRGENGPARRSLATAMTLDPASSAAWRKLGRLENTEKHVDAAESALRKAVTI
                     EPDGWQNHQSLGTFFYQKGRFEEALAEYERARELTPDNVLTLNTLAAVYHQLDRTDDA
                     AVALQRAMEIEPTFRGFNNLGVLHYFSGRYPQAAEAFQKSLDLGAGTYLNWGNLADAR
                     RQLGQKEAAAEAYTRAIQLARDHLATDASDREAAAMLAVYLAKRGNLDQARTQLTALA
                     PTSTDRSDDLFQASVAWEVIGNRPEALKHLELALDAGYGTREVKNEPELLKLREDPEY
                     HRLMSRFEKP"
     gene            complement(3151..3714)
                     /locus_tag="VF720_02500"
     CDS             complement(3151..3714)
                     /locus_tag="VF720_02500"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002535778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7878249.1"
                     /translation="MAREVRPLYVTTHETPLGTVGLAATPVGLASLMLRVNAAGFTEY
                     LRNSHGVMPVEDPQPFDRVRLFLDRYFAGKQIAFEGPWDLLVGTPFQRRVWATLLTIP
                     YGEVRSYRWLAETVGHPGAYRAVGAANGANPVAVLVPCHRVVNHNGGLGGYSGGLDIK
                     RWLLALERVPLAALTPQLRMESLVGHH"
     gene            3845..4597
                     /locus_tag="VF720_02505"
     CDS             3845..4597
                     /locus_tag="VF720_02505"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX7878250.1"
                     /translation="MNQQEVIQEMIEVGRRLDALGFVPATDGNLSVRLSSREILATTS
                     GVKKGMLTPRDFVVIDPDGETLRGGGRVSTEIQMHLRIYRDRPDAMAVVHAHPPTATG
                     FAVAGIPLTACVLPEVVATIGSVPLAPYATPSTSDLPDSIAPYIVDHDAVLLANHGVV
                     VFGPSLFDAANRMETVEQFAKILFVATTLGQVNRLTGGQVEELNQIRHRYGLAGRAAG
                     CDPGVPAPPEVSPPRALVDQVLAEVLKQLQGK"
     gene            4790..6616
                     /locus_tag="VF720_02510"
     CDS             4790..6616
                     /locus_tag="VF720_02510"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7878251.1"
                     /translation="MSDQPGLNIEHGFELIPGGAPGASSRPASPEPVDEPRLPAAAEL
                     KPVVEFARALAKAVKAARMYPANNPIYARFQTDLSERTANCLAEYSGIRLVVGQNRLF
                     SQGECVYESGDPEDNLARLFFRDGIRELTFHGGIDRAEMGRFLDMVRAAATRESTEDL
                     VTQLWDDSLPHITYIAIDDILDQELGQEPVPAEFGSDFMNYVDFEMDFAELDDDTVPE
                     AAREEAVTRAGELHRQLMESGDRQILSLTATEEEQLRPEVEEEDASSLVLRVLDTFFD
                     VIEQDNDPATRGVIFAVLEKALETLVAQRQWQAAGHILHAVGDVMVRREDLVLMHREA
                     INSILRASTDAPRCEALAEALNRTTSTAGESEETYLSAIQPGAVPALCELLGTVESRR
                     GRMMVVNTLTRIGQGNLDVLVGYLRDERWFVVRNILLVLGRMKASVAVKHIRPLVTHE
                     DLNVRREALAALSQIGEGEALDALLALLRDSDPRIRVSAARSLARLGKRAVNPLLQII
                     LARDFEARPLEERRGFFESLGRTNSPDVLPLLKMLLGKKPLFKKKEAEEMRVCAVEAL
                     ARMRGSEVKALLELALTDPSSVVRAAVAGAQKRQDEEDPDDV"
     gene            6609..8141
                     /locus_tag="VF720_02515"
     CDS             6609..8141
                     /locus_tag="VF720_02515"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7878252.1"
                     /translation="MSDRGPAVRNDLGLTRVLESGIHGMARDVIRGLNVAMRTARIHD
                     LKNDSVTAAIDSLVLILNDLFRVGGTFSIKALGDYLFWNDTRLKSDAGSQPVFDSVCR
                     EFRSRGIGSLTVHQGVDREAVFHLVQVLLTAPLAVVEEPDADPPAKGADHAAMVARAD
                     ESCRFLNERLHEKTPGIEFGPWRPTDLEELEKEQIDHKERAKKTFFRAVAVTRAVMTS
                     NKISQQMDIRKAKRVVQSMVDLIMEEEFSMLGLTTLKSHDDYTFFHSVNVCVLSIAIG
                     KRLGLDRKRLSELGVAALLHDIGKTEIPLTVIRKPGKFDKEEWDLMRRHPALGVRVLV
                     RLKGFSDLAMKSIVVAFEHHLGVNLAGYPPLRHARPLHLFSRVVTVADCFDAMTTQRV
                     YSDGAKPRDKALSYMLSQSDKLFDTTILKVFVNLMGVYPIGTLVRLDSGQLAVVLSTP
                     PESIDPRRPRIKLITDTTGIEFDGPVVDLMDGGPDTAPPYRIAETIDATTVNIDTGRF
                     FL"
     gene            complement(8206..8901)
                     /locus_tag="VF720_02520"
     CDS             complement(8206..8901)
                     /locus_tag="VF720_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878253.1"
                     /translation="MKRWLSIAVVALACLPPLGSARGDVSLGWNDVGPAMTGTQTFDC
                     GTNEGADTLVAAFRAPGGVTKLLMMEAVVDFCAGPTGLPEWWRFDGCRAGALSATTTM
                     GPLQVYFGDYWQGRGDVDVDFSLLLANTGRIVVTITLDPEEAGPLQGSSWYYAFRLVF
                     DHRKTVGQDACDECERPACFVLNDVRLVWPEGETRLHNDFNGVSWQSPLLGCPFIVPV
                     EPSTWSGIKTLFD"
     gene            9176..9646
                     /locus_tag="VF720_02525"
     CDS             9176..9646
                     /locus_tag="VF720_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878254.1"
                     /translation="MRMRFGLLGLALCGLLTGCSQLSPATLQDLGSADKVVLITFRDG
                     ETLKGRIIEDQTVTFQTFGKIYRGEVERVSDAEIVLKNAYISTEYDKYRVQRERMEKG
                     TLRITDDQSERIVLPTYRIAKVERVSVNKAKTARYGIFWGFTASVVAAILTARL"
     gene            9655..11265
                     /locus_tag="VF720_02530"
     CDS             9655..11265
                     /locus_tag="VF720_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878255.1"
                     /translation="MIIRFRTLAAAFLLAAAATPAMGQTTNPPVTVPVQGPSGLDVLR
                     YGYVTFDRTLPGSRAKSMGGAGLALSPDVNGASLNPANFSLLSAPELSADTRWMSGSS
                     TATDSPEGFPSPLGTEFPIREYSPSLTRNYTYNDLAFGMPLIFLGLRAGVGATYRRLI
                     DFRSGSEERFVVESPFGEADFGAGEQYKGGVDSFSPSFSVSLASRFSLGATLNFMTGD
                     ITSEGDQGVTSFGQVVTSGFVKLDQEVNGTSVDFGGRFDLTPSLSFSGVLQTGHDLKF
                     TSGDDQFQGLLDPTDPDPTPIIYDRALLDHTLSVPAMYGLGMAWRTMNNRLTLAADYW
                     NRGWSKATFTRQGFSTLTVFPDSLNLGTNFTAVIPNGTETQKKAGFKDTNSFRIGGEW
                     LVKGDGGAGMTIPLRLGFRNEPRSFANIDTTEFNALVKRIDVAAGAANMPVAERQAII
                     ADAMDEIFQNGSLLLEGDDVPATTVTFGTGIKVDSFSFDLGLAWTSYEFTHLFLGSFN
                     DFTRNIHIDTVTEDRSFTEMNFTATLRF"
     gene            11282..13357
                     /locus_tag="VF720_02535"
     CDS             11282..13357
                     /locus_tag="VF720_02535"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX7878256.1"
                     /translation="MIRTWQAAALILVVALGAVVAAPAATGPVIPGGRSNRPAVTWVF
                     FRDRPASLLAADPDAVDPAYVAEVRARVDRLRHVTRWLNGVSVEVNPDQLAELARLPF
                     VREVRPVLRGGRRHDLDRPALDPMGVNDPGIGPDTGAGGIDRFVPRLDSGPTPRSPGT
                     GGEVAFAGDEAWHYGASFNQNAQVGIVDLHRLGYTGAGVTVAIFDSGFRTTHVSLSGR
                     HVLAERDFVFGDDDVDNEPEDTPAAWNHGTSVWSNVGGFKPGALVGGAFEAGILLAKT
                     EDVRSETPIEEDNFIAAIEWADGLGADIISASLLYLDFDPGNPDYTYADLDGDTTPLA
                     IAVDMAVARGICMVNAVGNFGPNPGTLWTPADADSVLSVGAVDSLWIVTSFSSRGPTP
                     DGRIKPEVVARGRSNFVAFAAGGFGASSGTSFATPVTAGAAALLKEAHPSWTGWQLRE
                     ALRSTASHPGTPDNNTGWGLVRLVNAAGLSASSLPRVSRPFSLEFPDRGSVINSAAPV
                     FRWRRSIGNPGDVVTYRVALATTPTFAGADTFPAGLDSTWIPSMFLVPGTTYYWQVVA
                     TNLAGWKREALRPGSFTVAASVGVAERESGEPGPFLAAPAPNPFTGVTRLSMMLPPGE
                     WSVDVIGVDGRLVRRLARGTSEGIRSPQLLAWDGRTDDGMEAVAGIYFVRLLTPAGTS
                     SRRVVLERN"
     gene            complement(13365..14123)
                     /locus_tag="VF720_02540"
     CDS             complement(13365..14123)
                     /locus_tag="VF720_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7878257.1"
                     /translation="MDSARKDLLVDALLAYGLNEYEARAYLALLQHGSAVAGDISRRS
                     GIPRPRVYDTLQRLIEASLVTENGGSPRSYSPLPLDDFLGRMGAQFRRRQELLRDGLI
                     DPAGDNRNDGVFHVHDETPILRQAEDLIDAAQRHLDLRGSGIDLMPLARAMRDAAARG
                     VTIEGTTYGAAELPSGISLVAAPPADNRAGRGGRRLLIVRDREEVLIAELGGSNRPYA
                     VRSRNRLLVEMGAPGAAGVPNRHSPGTAAFAGGS"
     gene            complement(14315..>14620)
                     /locus_tag="VF720_02545"
     CDS             complement(14315..>14620)
                     /locus_tag="VF720_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878258.1"
                     /translation="RPDDPRAFAELGSAWNSLERYDLAEAALWRAIALDDKAAAPRIH
                     LARTLGMLGRERDARQQLELARPLMRSGDPLAGEFQLLDANLPPLYPTRAEPDGSHR"
BASE COUNT         2363 a         5028 c         4789 g         2440 t
ORIGIN      
        1 gggactcagc aacgacccgg cgctcatcga ttgggtagcc ggcgtcctca cgcccgtcgt
       61 cggcccggac gcggtcatcc acctcccgaa accgatcatg gggggcgaag actttgccca
      121 ctacctcgag gtgatgaccg gcgcgttcct ccgcatcggc gtgcgcgacg agtcgcgcgg
      181 gatcgtccac ccgctccaca gcccgcgctt cgatctcgat gaagcggctc ttccgtttgg
      241 cgcaggggtg ctggccacgg tggcacgcgc gtggctcatc cgggcggccg aagcgcgttg
      301 aaaggggtgc gcggcggacg cgaaatcagc cgatgtctcc cacgtccatc ttcggatcgt
      361 gctggcacga gtaatgggtg ccgctgcccg gatcgacgag gtcgaggctg atcgcgtatt
      421 cccagctccc cttgttggaa atggccaggg cattgccatc ggtgccgttg gcgagcgagc
      481 cgccctggac cccggtgttg ttgaacaccc agctcccggt cccgctgcca aggttgtcga
      541 aggggctggc gttgctggtg ccgggctggg cggtgtacgt cagccagaac tggttgaccc
      601 ggatcgcctg gttgacgtcg ttccagacga acgtggtcgt ggccttgttc ttgaccttcg
      661 ggaggttggc ctcctggaag ttcccccagc tctcgccgac gatcgggttg ccgttctcgt
      721 cgtaggcggt gagggtcatg ttccgcaccc gggcctgtgg ggtcgactgc gtgacatccg
      781 tggtgatgac aacgacgacg cttccaacct ttgtgttgtt gtcggacatg acgaacctcc
      841 tcaacgtttg ggcttcgatc agggtttttc gaaacgcgac atcagccgat ggtattcagg
      901 gtcctcacgg agcttgagca gctccggctc gttcttcacc tcgcgcgtgc catagccggc
      961 gtcgagggcc agctcgaggt gcttcaaggc ttcggggcga ttgccgatca cctcccacgc
     1021 cacgctggcc tggaagaggt cgtcggagcg gtccgtggag gtgggggcaa gcgccgtgag
     1081 ctgcgtcctg gcctggtcga ggttgccgcg ttttgccagg tacaccgcca gcatcgccgc
     1141 cgcctcgcga tcggaggcat cggtggccag gtggtcgcgc gccagctgga tggcccgcgt
     1201 gtaggcctcg gccgcggcct ccttctggcc cagctggcgt cgcgcatcgg ccaggtttcc
     1261 ccagttgaga tacgtgccgg ccccgaggtc gagcgacttc tggaaggcct cggccgcctg
     1321 tgggtagcgt cccgaaaaat agtggagcac gccgaggttg ttgaagccgc ggaaggtcgg
     1381 ttcgatctcc atggcccgct gcagggccac cgccgcgtcg tcggttcgat cgagctggtg
     1441 atagacggcg gccagcgtgt tcagcgtcag cacgttgtcg ggggtcagtt cgcgcgcgcg
     1501 ctcgtactcc gccagcgcct cctcgaaccg ccccttctgg tagaagaagg tccccagcga
     1561 ctggtggttc tgccagccgt ccggctcgat cgtcaccgct ttccggaggg cggattccgc
     1621 ggcgtcgaca tgcttttcgg tgttctccag gcgcccgagt ttccgccagg cggcactgct
     1681 ggccgggtcg agcgtcatcg ccgtggccag ggacctgcgg gcgggcccat tctcgccgcg
     1741 ctgcagctcc acggttgcca tcgcgatgtg cgagagggcg agctggtcgt tccggcggag
     1801 cgcctccgcc gccgccgctt cggcctggcg cagccagtgc tcgtccttgg cggccagaaa
     1861 cttctggtga caggcctcgg ccagccccgc ccacgccggc gccgagttgc tgtcggcggc
     1921 gatggccgaa tggaaaagaa cgatcgactc ctcgacattc ttcggacggt cgtaacggtc
     1981 gaggcgccgg cgggcctcgg tgaccagctc ggccgacccc ttcgggccat ccggcgcggc
     2041 cgttaccggt tgcttcgtcc ggatcagcgc ctggtacgcg agtccgccac cgacgatcgc
     2101 ggccaggagc accgccgcga ggatcagggg atcccgtccc gtccgcctgg ccggaagcgt
     2161 ggcatccgtc atggacccca ggggcgccgg gctcacagcg ggtgccatcg tgacggtcgg
     2221 cgcatccgtc aacggcagct cacgctcaat cgaccggagc gccgcggcga tcccggccat
     2281 cgcaaggcga cgatcgtcac ggtcgcgacg gaggcagttg cgtaccaggg aatcgagccc
     2341 ggccggcatt tcgccgcggt gggacgccag cggcgggggc tcggccacga gcaggcgctg
     2401 gagcgtgttc accgcggtat cgccccggaa cgggttgata ccgctgagca tctggtagag
     2461 gacgatgccg aacgaaaaga catcggtccg cgcgtcgagc gtctctccgg ccgcctgttc
     2521 cggcgacatg taggcgatgg tgccgctgac cgtgccggcc atggtaagcg gcgcggtgag
     2581 cgtggcgtcg tcggcgccga ccgcggatgc acccgcttcg gctccgggag cggggcgcat
     2641 ttccaccgcg cgagccaggc cgaagtcgag gatcttgatc cgcccgtcct cgttgaccat
     2701 cacgttggcc ggcttgagat cccggtggat gatccccgcg gcatgcgcct tggccagcgc
     2761 gtcggccatc tgcgcgcccc agtcgagcgc cttcccgatc gggagcccgc cggccgggat
     2821 gagctcggcc agctggcggc cggtgatgta ctccatcgcg atgacatcga cgccgtccgc
     2881 ggtgccgatc tcgtagatcg tgacgatgcc gggatggttg agcgacgacg ccacgcgggc
     2941 ttcccgttcg aaccggcgac gttcgatcgg atcggccagc cggtcgggag gaaggacctt
     3001 gagcgctacg gggcggtcga ggcgcgggtc gtgtccctgg tacacgacgc ccatgcctcc
     3061 ctggccaagg cgagcgacga cctggtagcg gaggagcgtg cggccgatca tcaccgcccg
     3121 attgtacgct gaacgggaaa ggggaggagc ctagtggtgt ccgaccaggc tttccatgcg
     3181 gagctggggc gtgagcgccg ccaggggaac ccgctccagg gccaggagcc agcgcttgat
     3241 gtccagcccg ccggaatagc ccccaagtcc gccattgtgg ttgaccaccc ggtggcaggg
     3301 cacgaggacg gccaccgggt tggcgccgtt ggcggctccg accgcccggt aggcgccggg
     3361 gtggccgacg gtttcagcca gccaccggta gctgcgtact tcgccgtagg gaatggtgag
     3421 gagcgtggcc cacacacggc gctggaatgg tgttccgacg aggagatccc acggcccttc
     3481 gaacgcgatc tgcttacccg cgaagtagcg gtcgaggaag agccgcaccc gatcgaacgg
     3541 ttgtggatcc tcgacgggca tcacgccgtg gctgttccgg agatactcgg tgaatcccgc
     3601 ggcgttgacg cggagcatga gcgatgcgag gccgacgggt gtggcggcga gtccgacggt
     3661 gccgagcggg gtttcgtggg tcgtgacgta gagcggtcgg acctcacgag ccatgctgtc
     3721 gtgcctttcc gtgtggcgga catcgcggtg gtgcacggcg cgagcgcgcc gaagggggac
     3781 gaaggctatg gtaatctcat cgcccgttcg acgcaacacg attgacgcga accatgaagg
     3841 tgcgatgaat cagcaggaag tcatccagga aatgatcgaa gtcggccggc ggcttgatgc
     3901 gctcggcttc gttcctgcca ccgacggcaa cctgtcggtg cggctgtcgt cgcgcgaaat
     3961 cctggccacg acgtccggcg tgaagaaggg gatgctcacg ccgcgcgatt tcgtcgtcat
     4021 cgatccggat ggggaaacgc ttcggggcgg tggccgtgtc tcgacggaaa tccagatgca
     4081 cctgcgcatc tatcgggatc gccccgatgc catggcggtc gtgcacgcgc accctccgac
     4141 cgccaccggc ttcgcggtgg ccggcatccc tctgacggcc tgcgtgttgc ccgaggtggt
     4201 ggcgacgatc ggatctgttc cattggcgcc gtacgccact ccttccacga gcgacctgcc
     4261 cgattcgatt gccccgtaca tcgtcgacca cgacgcggtg ctcctggcca accatggcgt
     4321 cgtggtcttc ggcccgtcgc tcttcgacgc cgccaaccgg atggaaacgg tcgagcagtt
     4381 cgcgaagatc ctcttcgtgg ccaccacgct cggccaggtg aatcgcctga ccggcgggca
     4441 ggtggaggaa ctcaaccaga tccgccaccg ctacggcctt gcgggacggg cggccggctg
     4501 cgaccccggc gtgccggcac cccccgaggt gtcacccccg cgcgcccttg tcgaccaggt
     4561 ccttgccgag gttctgaaac aactgcaagg caagtaacag taagcagttg ccgccccggc
     4621 gcacgggggc cggccatgcc gcttccggga cgctcgcgcc cgccggatcg ggtgatatcg
     4681 ctaacgtccc tcccgcgacg gtcgatattt ctcccagtac cactgcgctg acgaaaaacg
     4741 agctcgatgc gcctgccggt cccatccgac gcggcagaga tggggagaca tgtccgacca
     4801 gcccggcctc aacatcgaac atggcttcga actcattccg ggcggcgcgc ccggtgcgtc
     4861 gtcacgcccg gcctcgccgg aacccgtcga cgagccccgg ctccccgccg cggccgagct
     4921 gaaaccggtg gtcgaattcg cccgggcgct cgccaaggcg gtcaaggccg cccgcatgta
     4981 ccccgccaac aatccgatct acgcccgctt ccagaccgac ctctcggagc gcaccgccaa
     5041 ctgcctcgcc gagtacagcg gcatccggct ggtggtcggg cagaaccgcc tcttcagcca
     5101 gggggaatgc gtctatgaaa gcggcgatcc cgaggacaac ctcgcccgcc tcttcttccg
     5161 ggacggcatc cgcgaactca cgtttcacgg cggcatcgat cgggccgaga tgggacgctt
     5221 cctcgacatg gtgcgcgcgg ccgccacgcg cgaatcgacc gaggacctgg tcacccagct
     5281 ctgggacgac tcgctgccgc acatcaccta catcgccatc gacgacatcc tcgaccagga
     5341 gctggggcag gagccggtgc cggccgagtt cgggtcggac ttcatgaact acgtcgactt
     5401 cgagatggac tttgccgagc tcgacgacga cacggtcccc gaggcggccc gcgaggaagc
     5461 cgtgacccgc gccggcgaac tccatcgcca gctgatggag tcgggcgatc gccagatcct
     5521 gagcctcacc gccaccgagg aggagcagct ccgcccggaa gtggaggagg aagacgcgtc
     5581 cagcctggtg ctccgggtgc tcgacacctt tttcgacgtc atcgagcagg acaacgatcc
     5641 cgccacccgg ggagtcatct tcgcggtgct ggaaaaggcg ctggagacgc tggtggcgca
     5701 gcgtcagtgg caggcggccg gacacatcct ccacgcggtg ggcgacgtga tggtccgtcg
     5761 cgaggacctg gtgctcatgc accgggaggc gatcaactcc atcctccgcg cctccaccga
     5821 cgccccgcgc tgcgaagccc tggccgaggc gctcaaccgc accacgagca cggcggggga
     5881 gagtgaagag acatacctgt cggccatcca gccgggcgcc gtgccggccc tctgcgagct
     5941 cctgggtacc gtcgaatcgc gccgcgggcg aatgatggtg gtgaatacgc tgacacgcat
     6001 cgggcagggg aacctcgacg tcctggtggg ctacctgcgc gacgagcgct ggttcgtcgt
     6061 tcgcaacatc ctcctcgtcc tcgggcgcat gaaggcatcg gtggcggtga agcacatccg
     6121 cccgctggtc acccacgaag acctgaacgt gcggcgcgag gccctggcgg ctctctcgca
     6181 gatcggcgag ggagaggcgc tcgatgccct cctggccctg ctccgggatt ccgatccccg
     6241 gatccgcgtg tccgccgccc gcagcctggc ccgtctcggg aagcgggcgg tgaatccact
     6301 cctgcagatc atcctggccc gcgacttcga ggcccggccg ctggaggaac gccgcggctt
     6361 cttcgagtca ctcgggcgaa cgaacagtcc cgatgtgctg ccgttgctca agatgctgct
     6421 gggcaagaaa ccgttgttca agaagaagga agccgaggag atgcgggtgt gcgccgtgga
     6481 agcgctggcc cgcatgcgcg gctccgaggt gaaggccctg ctcgagctgg cgctcaccga
     6541 tccaagctcg gtcgtgcggg cagccgtggc cggcgcccag aagaggcagg acgaggagga
     6601 tccggacgat gtctgatcgc ggacctgccg tccggaacga cctgggcctg acacgggtgc
     6661 tcgaaagcgg catccacggg atggcgcgcg atgtgatccg cggcctgaac gtggccatgc
     6721 gcaccgcccg catccacgac ctgaaaaatg actcggtgac cgcggccatc gacagcctgg
     6781 tgctcatcct caacgacctg ttccgggtgg ggggcacctt cagcatcaag gcgctgggcg
     6841 attacctgtt ctggaatgac acgcgcctga agagcgatgc cggcagccag ccggtgttcg
     6901 acagcgtctg ccgcgagttc cgctcgcgcg gcatcggcag cctcaccgtc caccaggggg
     6961 tggaccggga ggccgtcttc cacctggtgc aggtgctcct caccgccccc ctggccgtgg
     7021 tggaagagcc cgatgccgac ccgcccgcca agggcgccga ccacgcggcc atggtggccc
     7081 gcgccgacga gtcatgccgt ttcctgaacg aacgtctgca cgaaaagacg ccggggatcg
     7141 agttcgggcc gtggcgcccc accgacctcg aggagctgga gaaggagcag atcgaccaca
     7201 aggagcgggc caagaaaacg ttcttccgcg ccgtggccgt cacgcgtgcc gtcatgacca
     7261 gcaacaagat cagccagcag atggacatcc ggaaggccaa gcgcgtcgtg cagtcgatgg
     7321 tggacctgat catggaggag gagttctcca tgctcggcct caccacgctg aagagtcacg
     7381 acgactacac gttcttccac tcggtcaacg tctgcgtcct ctcgatcgcc atcgggaaaa
     7441 ggctggggct cgaccgcaag cgcctctccg agctcggcgt ggccgcgctg ctccatgaca
     7501 tcggcaagac cgagatcccg ctcaccgtca tccgcaagcc cggcaagttc gacaaggaag
     7561 agtgggacct gatgcgccgc cacccggcgc tcggggtccg cgtgctggtg cgtttgaagg
     7621 ggttctccga cctggccatg aagtcgatcg tggtggcctt cgagcaccac cttggcgtga
     7681 acctggcggg ctacccgccg ctcaggcacg cgcggccgct ccatctcttc agccgggtcg
     7741 tcaccgtggc cgactgcttc gacgccatga ccacgcagcg tgtctattcg gacggcgcca
     7801 agccgcgcga caaggcgctc tcgtacatgc tgagccagtc ggacaagctc ttcgacacga
     7861 ccatcctcaa ggtgttcgtg aacctgatgg gtgtctaccc gatcggcacc ctggtgcgcc
     7921 tcgactcggg ccagctggcc gtcgtcctct ccacgccccc ggaatcgatc gacccccgcc
     7981 gcccgcgcat caagctcatc accgacacga ccggcatcga attcgacggc cccgtcgtcg
     8041 acctgatgga cggcggcccg gacacggcgc ccccctatcg catcgccgag acgatcgacg
     8101 ccacgacggt caatatcgat acgggacgct tcttcctcta ggaacaccgc ggatcggcct
     8161 cggccgacga cacaggaccg gggggttcgg cggttgggcc gccgctcaat cgaacagtgt
     8221 cttgatgccg ctccaggtgg aaggctcgac ggggacgatg aaggggcacc ccagaagcgg
     8281 cgattgccag ctcaccccgt tgaagtcatt gtgcagccgc gtctctccct cgggccagac
     8341 gagccgcaca tcattcagga cgaagcaggc cggccgctcg cactcgtcgc acgcatcctg
     8401 acccaccgtc ttccggtggt cgaacacaag gcggaacgcg tagtaccagg agcttccctg
     8461 gagcggtccg gcctcctcgg gatccagcgt gatcgtgacg acgatccggc cggtgttcgc
     8521 caggaggagg ctgaaatcca cgtcgacatc accgcgaccc tgccagtagt cgccgaagta
     8581 gacctggagc ggccccatcg tggtcgtcgc cgacagggcg ccggcacgac atccgtcaaa
     8641 tcgccaccac tccggcagac ccgtaggtcc ggcacagaaa tcgacgacgg cctccatcat
     8701 caggagcttc gtcacgccgc cgggcgcccg gaaagcggcc accagcgtgt ccgccccttc
     8761 gttcgttccg cagtcgaagg tctgggtgcc ggtcatggcc ggcccgacat cgttccaccc
     8821 cagtgacaca tccccacggg ccgatcccaa cggtggcagg caggcaagcg cgaccacggc
     8881 gatcgatagc caacgtttca tggcgcctcc aatcgaacga gtgccggaca tggccgccgg
     8941 aggcggcctc cagatgttcc actcatcccg aaaaggtgga tcgaaacacc ccttccggcc
     9001 ggttttcttg cgttcgacgc cgcacacgtc cccgccttga caccccacgt tccagccggt
     9061 aacttttcgc ccgacaacgc ggtacccccc ctctcgatac gccacgggcc ccttgagaag
     9121 cccgcgcgcg aaatccttga agagccgttt ccgacggagg aggatcgtcg cccccatgcg
     9181 catgcgcttc gggctgttgg gcctggcgct ctgtggtctg ctgaccggtt gctcccaatt
     9241 gagcccggct actctccagg acctcggttc cgccgacaaa gtggtgctca tcacattccg
     9301 tgacggtgaa acgctcaagg gtcgcatcat cgaggaccag acggtcacct tccagacctt
     9361 cggcaagatc taccggggtg aggtggagcg ggtgagcgac gccgagatcg tgctcaagaa
     9421 cgcctatatc tccactgagt acgacaaata ccgcgtgcag cgcgaacgga tggagaaggg
     9481 aacgctgcgc atcaccgacg accagtcgga acggatcgtc ctgccgacct accgcatcgc
     9541 caaggtggag cgggtgtccg tcaacaaggc caagacggcg cgctacggga tcttctgggg
     9601 cttcacggcc tccgtggtgg ccgccatcct taccgcccgg ctctgaggtt cgcgatgatc
     9661 atccgattcc gtacgctggc cgccgcgttc ctgcttgccg ccgccgccac tcccgcgatg
     9721 ggccagacca ccaatccgcc tgtcaccgtt ccggtccagg gtccctcggg cctggatgtc
     9781 ctgcgatacg gatacgtcac cttcgaccgc acgctccccg gatcgcgcgc caagtcgatg
     9841 ggtggtgctg gccttgcgct ctcgcccgac gtcaacgggg ccagcctgaa tccagccaac
     9901 ttcagcctcc tttcggctcc cgagctcagc gccgatacgc gttggatgtc ggggagcagc
     9961 acggccacgg actcgcccga gggtttccct tcgccgctcg gcaccgagtt cccgatccgc
    10021 gaatattccc ccagccttac ccggaactac acctacaacg acctggcctt cgggatgccg
    10081 ctcatcttcc tggggcttcg cgccggtgtc ggggccacct accggcggct catcgatttc
    10141 cgcagcggct cggaagagcg gttcgtggtg gagagcccgt tcggcgaagc cgacttcggc
    10201 gccggcgagc aatacaaggg aggcgtcgac tccttcagtc cctccttctc ggtgtccctg
    10261 gccagtcggt tctcgctcgg cgccacgctc aacttcatga ccggcgacat cacctcggag
    10321 ggggaccagg gggtcaccag cttcggccag gtggtcacgt cgggcttcgt caagctcgac
    10381 caggaggtca acggcacctc ggtcgacttc gggggacggt tcgaccttac tcccagcctc
    10441 tccttttccg gtgtcctgca gaccggccac gacctcaagt tcaccagcgg cgacgaccag
    10501 ttccaggggc tcctcgatcc gacggacccg gaccccaccc cgatcattta cgaccgggcc
    10561 ctgctggacc acaccctttc cgttccggcg atgtacggcc tcgggatggc gtggcgaacc
    10621 atgaacaacc gtctcacgct ggccgcggac tactggaacc ggggatggag caaggccacg
    10681 ttcacccgcc agggcttctc cacgctcacg gtcttcccgg actcgctcaa cctcgggacg
    10741 aacttcaccg cggtcattcc gaacggaacc gaaacccaga agaaggcggg attcaaggac
    10801 accaacagct tccgcatcgg gggcgaatgg ctggtgaagg gtgacggtgg cgcgggaatg
    10861 acgattcccc tccgtctcgg tttccggaac gagccccggt cgttcgccaa catcgacacc
    10921 accgagttca atgccctcgt caaacggatc gacgtggcgg ccggggctgc caacatgccg
    10981 gtcgcggagc gccaggccat catcgccgat gccatggacg agatcttcca gaacggctcg
    11041 ctcctcctcg agggagatga tgtgccggcc acgaccgtca cattcggtac cggcatcaag
    11101 gtggacagtt tttccttcga cctcgggctt gcctggacga gttacgaatt tacccacctg
    11161 ttcctcgggt cgttcaacga cttcacgcgg aacatccaca tcgataccgt caccgaagac
    11221 cgcagcttca cggagatgaa tttcacggcc acgctccgct tctagttata ctcacagcct
    11281 catgattcgc acctggcagg ccgccgccct catcctcgtc gtcgccctcg gcgccgtggt
    11341 ggccgctccg gccgccaccg gccccgtgat cccgggcggc cgctcgaatc gccccgccgt
    11401 cacctgggtc tttttccgcg accgcccggc ctcgctcctg gccgccgatc ccgatgccgt
    11461 tgatcccgcc tacgtggcgg aagtgcgggc cagggtcgat cgactccgcc acgtcacgcg
    11521 ctggttgaac ggcgtcagcg tggaagtgaa cccggaccag ctggccgaac tggcacgact
    11581 gccgttcgta cgcgaggtgc gtccggtgct tcgcggaggg cggcggcatg acctcgatcg
    11641 tccggcgctc gacccgatgg gcgtcaatga ccccggcatc ggtcccgaca cgggcgccgg
    11701 cggaatcgac cggttcgtcc cgcgcctcga ctccggcccg acgccccgca gcccgggaac
    11761 gggaggagaa gtggcgttcg ccggcgacga ggcctggcac tacggcgcct cgttcaacca
    11821 gaacgcccaa gtcggcatcg tcgacctgca ccggctcggt tacacgggcg ccggcgtcac
    11881 ggtggccatc ttcgactcgg gattccggac gacgcatgta tccctgtcgg gccggcacgt
    11941 cctggccgaa cgggatttcg tcttcggcga tgacgatgtg gacaacgagc cggaagacac
    12001 gccggcggcc tggaaccacg gcacgtcggt ctggagcaac gtgggaggct tcaagcccgg
    12061 tgccctggtg ggcggcgcct tcgaggccgg catcctcctg gccaagaccg aggacgtgag
    12121 gagcgaaacg cccatcgagg aagacaactt catcgcggcc atcgaatggg ccgacgggct
    12181 cggcgccgac atcatcagcg cctcgctcct ctatctcgat ttcgatcccg gcaacccgga
    12241 ctacacctac gccgacctgg acggcgatac gaccccgctc gccatcgccg tcgacatggc
    12301 ggtggcccgc ggcatctgca tggtgaatgc cgtcggcaac ttcggcccga atcccggcac
    12361 gctctggacg cccgccgatg ccgactcggt cctctccgtc ggcgcggtcg attcgctgtg
    12421 gatcgtgacg tcgttctcct cgcgcggccc cacgcccgac ggtcgcatca agcccgaagt
    12481 ggtggcgcgc ggacggtcga atttcgtggc gttcgccgcg ggcggtttcg gcgcgtcgag
    12541 cggcacctcg ttcgccactc cggtcacggc cggcgcggcg gcgctcctca aggaggcgca
    12601 tccctcctgg accggctggc aactccgcga ggcgttgcgc tccacggcca gccatccggg
    12661 cacacccgac aacaacaccg gctgggggct cgtccgcctc gtcaatgcgg ctgggctttc
    12721 ggccagctcg ttgccccgcg tgtcgcgccc tttctcgctc gagtttcccg accgtggttc
    12781 cgtcatcaac tcggcggccc cggtcttccg gtggcggcgg tcgatcggca accccggtga
    12841 tgtcgtgacc tatcgggtgg ccctggccac cacccccacg ttcgccggcg ccgacacctt
    12901 cccggcgggc ctcgattcca cgtggatccc gtcgatgttt ctggtgccag gtaccaccta
    12961 ctactggcag gtggtggcca cgaacctggc cggctggaag agagaggccc tgcgccccgg
    13021 atccttcacc gtggccgcca gcgtcggtgt ggccgaacgg gagtcgggtg agcccgggcc
    13081 gttcctggcg gctccggccc ccaacccctt cacgggcgtc acgcgcctct cgatgatgct
    13141 tccacctgga gaatggtcgg tggacgtgat cggtgtggat ggccgcctgg tccggcgcct
    13201 cgcccggggg acgagcgaag gcatacgatc gccgcagttg ctggcatggg acgggcgcac
    13261 cgatgacggg atggaagccg tggccggcat ctacttcgtg agactgctaa cgccggccgg
    13321 aacctccagc cgacgggtcg tgctcgaacg caactgagcc gggatcagct cccgccggcg
    13381 aacgccgccg tgccgggcga gtgccggttc ggaacgccgg ccgctccggg agcccccatc
    13441 tccaccagca ggcgatttcg actccgcacc gcgtagggac gattcgagcc acccagctcc
    13501 gcgatcaaca cttcctcccg gtcgcgcacg atcaggagcc ggcgaccgcc gcgccccgcc
    13561 cggttgtcgg ccggcggcgc ggccaccagc gaaatcccgg aagggagttc cgccgcgccg
    13621 taggtcgtcc cttcgatcgt cacgccgcgc gcggccgcat ccctcatggc ccgggccaac
    13681 ggcatgagat cgatgccgga accccggagg tccaggtggc gctgcgcggc gtcgatcagg
    13741 tcctccgcct ggcgcaggat cggcgtttca tcgtgcacgt ggaagacgcc gtcattcctg
    13801 ttgtctcccg ccgggtcgat gagtccgtcg cgcaggagct cctgccggcg ccggaactgc
    13861 gctcccatgc gtccgaggaa atcatcgaga gggagagggg aatagctgcg gggtgagccg
    13921 ccgttttccg tcacgagcga ggcttcgatc aggcgttgca gcgtgtcgta cacgcggggg
    13981 cgtggaatgc ccgaacggcg cgagatatcg ccggcaacgg cggaaccgtg ctggagcagt
    14041 gcgaggtagg cgcgggcttc gtactcgttc aggccgtacg cgaggagcgc gtcgaccagc
    14101 agatccttcc gggcagaatc caatggcttc tcacaggttg tccccctgag gacgagcaaa
    14161 atctagcata ccttccgcat gccccactcg ttgccatgcc tttttttgat tcatgcacaa
    14221 cttttttcgc gcattggctt ccttccgacg agctttgttc aattctccga cagtgccggg
    14281 accccactca tgacaatccg ggcggccaat cagcttaccg atggctccca tcgggctcgg
    14341 cgcgggtggg gtacaggggg gggaggttgg catcgagcag ctggaactct ccggccaggg
    14401 ggtcgccgct ccgcatgagc gggcgggcga gttccagctg ctgacgggcg tcccgttcgc
    14461 gccccagcat cccgagcgtc cgcgccaggt gaatgcgggg ggcggctgcc ttgtcgtcca
    14521 gggcgatggc ccgccacaag gccgcctcgg ccaggtcgta gcgctcgaga ctgttccacg
    14581 cactccccag ctccgcgaag gcgcgcggat cgtccgggcg
//
LOCUS       DASVOD010000127         6021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_530092, whole genome shotgun sequence.
ACCESSION   DASVOD010000127 DASVOD010000000
VERSION     DASVOD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6021
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530092"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VF720_02550"
     CDS             complement(<1..501)
                     /locus_tag="VF720_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7878259.1"
                     /translation="MPQLFERSANAFARFTLFGSIFLIAGIGLVATEVNRSGYGTRAD
                     VFVDQPVPFSHQHHVGQLGLDCRYCHTSVEETASAGMPPTKTCMNCHSQVWTNAPMLE
                     PVRASFRTDQSIEWVRVHDLPDFVYFNHSIHVAKGIGCESCHGPVNNMPLVYKANTLF
                     MEWCIQC"
     gene            complement(609..1013)
                     /locus_tag="VF720_02555"
     CDS             complement(609..1013)
                     /locus_tag="VF720_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878260.1"
                     /translation="MPANPRFTLVRSKLVGPAAHHEQLLQLLNAPADYWKAQTPVPGK
                     PTGRIGLSADGASFSEFQVVEGVTLGQMETLLVLMAARMPQISVTFDVSGAGEVTGLR
                     LLLGKLEAGRSGAAQTVDWQPLPHHSTLIGSK"
     gene            complement(1020..1832)
                     /locus_tag="VF720_02560"
     CDS             complement(1020..1832)
                     /locus_tag="VF720_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7878261.1"
                     /translation="MNALADQLLSFPEVDLYVEQAGTGPAIVFIHDYTLDHELWHESV
                     VNLSSHSRCITYDLRGHGKSSSPSTGYALSDHVDDLFRILDSLSVDKATLVGLSMGAG
                     VALHAALMTPARVHRLVLAAPVLNGLPWEEGMWNWFREFETKARQIGVQRAIDQVWMP
                     GTLFKSIRRYPALNQRIREMAARFTGGNIFDRTVYPRPALTDRERLSEVTCRTLVLRG
                     EFDRPEFVRRAQMLADGIPGARLEMVPRAGHFPNLEAPAAFHRSVTRFLKEE"
     gene            1883..2356
                     /locus_tag="VF720_02565"
     CDS             1883..2356
                     /locus_tag="VF720_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878262.1"
                     /translation="MAKVVPSRDEGLLLLAAIRVRAHLDGRPPLQENVADLLDMPHEL
                     MRVLAIGLEDRGILKMLTNAFEVRLDIVNAALVDDLPAAGTGPRLGTELDDFRKAFQA
                     KQDDLKKSFGKEALQKKTDEKMSAMEEKLRQFKEKAGGKPMFGMLSDRPPDDDDD"
     gene            2369..2875
                     /locus_tag="VF720_02570"
     CDS             2369..2875
                     /locus_tag="VF720_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX7878263.1"
                     /translation="MAKTQSFDVTTGVDYNEVQNAVQQAMKEIGQRYDFKGADVSLDV
                     KEEENKLILQAPDDYKVRAAWDILQTKLIRRGQPAKNFEPGDPQPAAKGSLRQEIAIT
                     NGLSSDLAREVVKYLKDAKMKKVQAAIQGDTVRISGPSRDDLQEAIALLKGHDFGIEL
                     KFENFRSQ"
     gene            complement(2882..3379)
                     /locus_tag="VF720_02575"
     CDS             complement(2882..3379)
                     /locus_tag="VF720_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009633282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX7878264.1"
                     /translation="MKKHPELDEPSAKLRDLIEDIDFCMLTTLEPDGSLHSRPMSTQE
                     MDAEGCLWFFTSTDSAKVREVRADEHVNLNYARPKDMRYVSVSGTAEVVRDPAKNKEL
                     WKPIYKTYFPEGLDDPNLGLLKVHVHEAEFWDDSSNRMVKFFKFVKSAVTGTAYEADH
                     GKMQM"
     gene            complement(3569..5566)
                     /locus_tag="VF720_02580"
     CDS             complement(3569..5566)
                     /locus_tag="VF720_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX7878265.1"
                     /translation="MPAHAPIPAASRCVRRLVFPLALLTAGLMAAQPARALKVMNYNL
                     LNYPSATGAAREDEFRTILTAVHPDIMMCQEVTGSTSAGATQYLNNVLNTAFPGEYAL
                     AQFIDGDDTNNALFYRTAAFDFVSADTIATPLREIHHWVMQPDGYAASAAQIHFYAAH
                     LKAGSTGADQTQRADECQRWRNRMNTHAAGTNVIGGGDFNIQSSSETSYSTWLIGSLA
                     DNDGRLFDPINTPGSWNNNATYAGIHTQSPRTAANPYGGATGGMDDRFDFLLISSAMN
                     DNEGMAYVPGTYKCYGNDGQHLNLAINALPTIPEGATIANALEEASDHMPVIMTIQVP
                     ARIDVVASLNFGSVIVGATANQSLNIANIGVAPADELTYTFTPPAGFTAPGGTQNLNA
                     GFNINHTIGMTTGSFGNKAGNLSIATDDVDNATKLVALSGTVLRHAVPSVANAPIATL
                     DTLDFGNEPIGMFVDGMVQAANAGYTGLQARLDVYGASITGADAARFSIVGGFNAALV
                     AGTPVDFTVHFDDTGAADNTTYVADLTFTTRDENLPGQANQASIVWHLTAHTEAASGV
                     PGDGSLPTVSRLVGNYPNPFTPSTRLAFEIAEHGFATIGIFDVNGRLVRRLVEAPFSP
                     GRYEQVWDGTDDAGRPVSQGVYFYCLTTPVKRETKTMTLVR"
     gene            complement(5671..>6021)
                     /locus_tag="VF720_02585"
     CDS             complement(5671..>6021)
                     /locus_tag="VF720_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878266.1"
                     /translation="VEVRVANLKALFFPRADAAPLLSRNSQPAYSQAIDPVRDGKRIV
                     VRFQDGETMTGFALSYRPEKVGFFLFPDDPDSQHEKIFILQAATSEVLVGEKAQAHPV
                     ATRTATPTSQDKAA"
BASE COUNT         1050 a         1824 c         2085 g         1062 t
ORIGIN      
        1 gcactggatg caccactcca tgaagagcgt gttggccttg tagaccagag gcatgttgtt
       61 caccgggccg tggcacgact cgcaaccgat ccccttcgca acgtgaatgg aatgattgaa
      121 atagacgaag tcgggcaggt catgcacccg gacccattcg atcgactggt cggtgcggaa
      181 actggcccgg accggctcga gcatcggcgc attggtccag acctgggaat ggcagttcat
      241 gcaggtcttg gtgggaggca tgccggccga ggccgtctcc tccaccgagg tatggcaata
      301 ccggcagtcc agtccaagct gacccacgtg gtgctggtgg ctgaagggga ccggctggtc
      361 gacgaagaca tccgcccgcg tgccgtagcc cgaccggttg acctcggtcg ccacgagtcc
      421 gatgccggcg atcaggaaga tcgacccgaa cagcgtgaat cgtgcaaagg cattggcgct
      481 gcgttcgaag agctgcggca ttcggccctc atgaccattt gacaggtacc ggcaaaaacg
      541 tgcgcatctt agtccgggtc acccggatgc tcaagcgcaa atcgttcttt tgtttattcg
      601 gaattggact acttggaacc gatcagcgtg gagtggtgcg gaaggggctg ccagtcgacg
      661 gtttgcgcgg ctccggaccg tccggcctcg agcttcccga gcaggaggcg caggcccgtg
      721 acctccccgg cgccggaaac atcgaaggtc accgagatct ggggcatacg ggccgccatg
      781 aggaccagga gcgtctccat ctggccgagc gtcacgcctt cgaccacctg gaactcggaa
      841 aacgaggcgc catcagcgga caggccgatc ctgccggtgg gcttgccggg aacgggagtc
      901 tgcgccttcc agtagtcggc gggcgcgttc aggagctgga gcaactgctc gtgatgggcg
      961 gccgggccga ccagcttcga gcggacgagc gtgaaacggg gattggcggg catcctcggt
     1021 cactcctctt tcaggaatcg ggtcaccgac cgatgaaacg cggccggggc ttccaggttg
     1081 ggaaaatggc cggcccgcgg caccatctcg aggcgtgccc cgggaatgcc gtcggccagc
     1141 atctgggccc gccgcacgaa ctcggggcgg tcgaactccc cgcggaggac cagcgtcctg
     1201 caggtaactt ccgacaaccg ctcacggtcc gtcaatgcgg gccgcggata gacggtccga
     1261 tcaaagatat ttcccccggt gaaacgggcg gccatctcgc ggattcgctg gttgagcgcg
     1321 gggtagcgcc ggatcgattt gaaaagcgtc ccgggcatcc agacctggtc gatcgcgcgc
     1381 tgcaccccga tctggcgggc cttcgtctcg aactcgcgaa accagttcca catcccctct
     1441 tcccagggaa gcccgttgag gacgggcgct gccaggacca gccgatgcac gcgggccggc
     1501 gtcatgagcg ccgcgtggag ggccaccccc gctcccatcg agagccccac gagtgtggcc
     1561 ttatccaccg agagggaatc caggatccgg aagagatcgt ccacgtgatc cgaaagggcg
     1621 tagccggtgc tcgggctcga cgacttgccg tgcccccgga ggtcgtaggt gatgcagcgg
     1681 ctgtggctgg agaggttgac gaccgactcg tgccacaatt cgtggtcgag cgtgtagtcg
     1741 tggatgaaga cgatggccgg tcctgtgccg gcctgttcca catagaggtc gacctccggg
     1801 aaggacagca gctgatcggc cagggcattc atgaggccgc cgattctagc aggccccgcc
     1861 cccgcctgtt agcatcggcc tcatggcgaa agtggtcccc tcccgcgacg aaggcctgct
     1921 cctcctggcc gccatccgcg tccgcgccca cctggacggc cggccgccat tacaggaaaa
     1981 cgtggccgac cttctcgaca tgccccacga actgatgcgg gtcctggcca tcggcctcga
     2041 ggacaggggt attttgaaga tgctcaccaa cgctttcgag gtgcgtctcg acatcgtcaa
     2101 cgcggccctg gtcgacgacc tccccgccgc cggcaccggc ccccggctcg gcaccgagct
     2161 cgacgatttc cggaaggcct tccaggcgaa gcaggacgac ctcaagaagt cgttcggcaa
     2221 ggaggccctc cagaagaaga ccgacgaaaa gatgtcggcc atggaggaaa agcttcgaca
     2281 gttcaaggag aaggccgggg gcaagccgat gttcggcatg ctgtcggacc ggccgccgga
     2341 cgacgacgac gactgagggg gtttcggagt ggccaagaca caatcgttcg acgtgaccac
     2401 cggcgtcgac tacaacgaag tgcagaatgc cgtccagcag gcgatgaagg aaatcggcca
     2461 gcgttacgac ttcaaggggg ccgacgtctc tctcgacgtg aaggaagagg agaacaagct
     2521 gatcctccag gcgcccgacg actacaaggt ccgggccgcc tgggacatcc tgcagaccaa
     2581 gctcatccgg cgcggccagc ccgcaaagaa cttcgagccc ggcgatccgc aacccgccgc
     2641 aaagggttcg ctccgccagg agatcgccat cacgaacggc ctctcgagcg acctggcccg
     2701 cgaagtggtg aagtacctga aggacgccaa gatgaagaag gtgcaggccg ccatccaggg
     2761 cgacacggtg cgcatctccg gcccgtcacg cgatgacctc caggaggcca tcgcgctgct
     2821 gaaggggcac gacttcggga tcgagctgaa gttcgagaat ttcaggagcc agtagccgcc
     2881 gctacatctg catcttgccg tgatccgcct cgtacgcggt accggtcacg gccgacttca
     2941 cgaacttgaa gaacttgacc atcctgttgg acgagtcgtc ccagaactcg gcttcgtgca
     3001 cgtgcacctt gaggagcccg aggttcgggt cgtcgagccc ctccgggaag tacgtcttgt
     3061 agatgggttt ccacagttcc ttgttcttgg cgggatcgcg aaccacttcg gccgtgccgg
     3121 aaaccgacac gtagcgcatg tccttcggcc gcgcgtagtt caggttgacg tgctcatcgg
     3181 cgcgcacctc gcgcaccttg gcgctgtcgg tcgacgtgaa gaaccagagg cacccttcgg
     3241 cgtccatctc ctgggtggac atggggcggc tgtggagcga gccatcgggc tccagcgtcg
     3301 tcagcatgca gaagtcgatg tcttcgatca ggtcgcgcaa tttcgcgctc ggctcgtcga
     3361 gttcgggatg ttttttcatg gtggggctcc tcgaatcggg ttggctcgta cggcccggga
     3421 ggatgaaact cgcaaaggtc gtgccgggga atgagatccc gcctgtcgat gtcgaagggc
     3481 attgaattga aacggccacc ctttcgggtg gccgttgctc gtcatcaaga cgtgaaaaaa
     3541 gtgctcgggc gctcacgcgc cctgcgcgtt accggaccag cgtcatcgtc ttcgtctcgc
     3601 gcttgaccgg tgtcgtgagg cagtagaagt acacaccctg gctcacgggg cgaccggcgt
     3661 cgtccgtgcc gtcccacacc tgctcatagc ggccggggga gaagggggct tcgacgaggc
     3721 gacgcaccag ccggccattc acgtcgaaga tgccgatcgt ggcgaagccg tgctcggcga
     3781 tctcgaacgc caggcgggtc gagggcgtga aggggttcgg gtagttgccg acgaggcggc
     3841 tgaccgtggg gagcgatccg tcgcccggga cgccggaggc ggcctcggtg tgggcggtca
     3901 ggtgccagac gatgctggcc tggttggcct ggccgggaag gttctcgtcg cgcgtggtga
     3961 aggtgaggtc ggccacgtag gtggtgttgt cggcggcgcc ggtgtcgtcg aaatgcacgg
     4021 tgaagtcgac gggggtgccg gccaccagtg cggcgttgaa gccgccgacg atcgagaagc
     4081 gggccgcatc ggcaccggtg atcgaggcgc cgtacacgtc cagccgggcc tgcagcccgg
     4141 tatatcccgc gttggcggcc tgcaccatgc cgtccacgaa catgccgatc ggttcgttgc
     4201 cgaaatcgag cgtgtcgagc gtggcgatcg gcgcgttggc caccgacggc accgcgtggc
     4261 ggagcacggt gccggaaagc gccacgagtt tcgtcgcgtt gtccacatca tcggtggcaa
     4321 tgctcagatt gccggccttg ttcccgaacg agccggtggt catcccgatg gtgtggttga
     4381 tgttgaagcc ggcattcagg ttctgcgtgc ccccgggcgc ggtgaagccg gccggaggcg
     4441 tgaaggtgta ggtgagttcg tccgccggtg ccacgccgat gttggcgatg ttgagcgact
     4501 ggttggccgt ggccccgacg atcaccgaac cgaaattgag cgaagccacc acgtcgatcc
     4561 gggccggcac ctggatcgtc atgatcaccg gcatgtgatc ggaggcttcc tcgagcgcgt
     4621 tggcgatcgt ggctccctcg gggatcgtgg gcagggcgtt gatggccagg ttcaggtgct
     4681 gcccgtcgtt tccgtagcac ttgtaggtgc ccggcacgta ggccattcct tcgttgtcgt
     4741 tcatggccga ggagatgagg aggaagtcga accggtcgtc catcccgccg gtggcgccgc
     4801 cgtacgggtt cgcggccgtc cgcgggctct gggtgtggat gccggcgtag gtggcgttgt
     4861 tgttccagct ccccggtgtg ttgatcgggt cgaacaggcg cccgtcattg tcggccaggc
     4921 tgccgatgag ccaggtgctg tacgacgttt ccgaactgct ctggatgttg aaatcgccgc
     4981 ctccgatgac gttggtgccg gccgcgtgcg tgttcatccg gttgcgccag cgctggcact
     5041 cgtcggcgcg ctgggtctgg tcggcgccgg tgctccccgc cttgaggtgc gccgcataga
     5101 aatgaatctg cgcggcggag gccgcatagc cgtccggttg catcacccag tgatggatct
     5161 cgcgcagggg cgtggcgatc gtgtcggcgg acacgaagtc gaacgcggcc gtgcggtaga
     5221 agagtgcgtt gttggtgtcg tcgccgtcga tgaactgggc cagcgcgtac tcgccgggga
     5281 aggccgtgtt caggacattg ttcaggtatt gcgtggcgcc ggccgaggtg ctcccggtta
     5341 cttcctggca catcatgatg tcgggatgga ccgccgtcag gatcgtccgg aactcgtcct
     5401 cgcgggcggc acccgtggcg gacgggtagt tgaggaggtt gtagttcatg accttgaggg
     5461 cccgggcggg ttgggcggcc atcaggcccg ccgtcaggag tgccaggggg aaaacgaggc
     5521 gccggacgca acgggacgcg gccgggatcg gtgcgtgggc aggcatgaac gcatctccgg
     5581 gaggtgtcga gaggccctgc tttcgagggt gcgattcgat gatgaagagt atatcacccg
     5641 gccggaggaa gcccgcccgg gaggcgcctg tcaggctgcc ttgtcctgcg aggtgggcgt
     5701 ggccgtgcgg gtggccaccg ggtgcgcctg ggccttctcg cccaccagga cttcgctggt
     5761 cgcggcctgg aggatgaaga tcttctcgtg ctggctgtcg ggatcgtccg gaaagaggaa
     5821 gaaccccact ttctccgggc gatacgaaag ggcgaagccg gtcatcgtct ccccgtcctg
     5881 gaagcgcacc acgatgcgct tgccgtcccg tacgggatcg atcgcctgcg agtaggccgg
     5941 ttgggagttg cgggagagga gcggagccgc gtcggcgcgc ggaaaaaaga gcgccttcag
     6001 gttggccacc cggacttcca c
//
LOCUS       DASVOD010000128         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_321583, whole genome shotgun sequence.
ACCESSION   DASVOD010000128 DASVOD010000000
VERSION     DASVOD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321583"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="glp"
                     /locus_tag="VF720_02590"
     CDS             complement(<1..1023)
                     /gene="glp"
                     /locus_tag="VF720_02590"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX7878267.1"
                     /translation="MLTLPEAKQRILAALPVMPVLETSFTAALGHILAEDVVAGEDLP
                     PFANSSMDGFAVRAADLAAASSESPARLLVVDDLPAGRAPTRGVAAGETIRIMTGAPI
                     PPGADAVAIVETSQPDGQHVLIDKPVRAGANIRLAGESVPRGSLALAAGSLVRAAEVG
                     LLASLGVTRVRVFGKPVVALVSSGDELVPPDSAPGPGQIRDSNRFTLAAHLASLGYTV
                     LDGGNAPDVESELEDHFRRAIAAADVVVSTGGVSVGDHDLTRIVLSRLGTMDFWRVAI
                     RPGKPLAFGFIEGKPVFGLPGNPVSSLVVLDQIVRPALRRMAGHQRTDRETWKAVLEE
                     PIRRAPG"
     gene            complement(1017..>1610)
                     /locus_tag="VF720_02595"
     CDS             complement(1017..>1610)
                     /locus_tag="VF720_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEX7878268.1"
                     /translation="RAGFEGRRRERPLRPRTATPAPRMTTTTFRAAIITVSDSSSRGE
                     RPDVSGPEARAAIERLGGEVTSTEILPDDRAVVSERLARLADSGTFALIVTTGGTGFS
                     RRDVTPEATRDVIEREAPGLAELMRRDTAGLTPLAPLSRGVCGLRAGTLIVNLPGSPK
                     GVRECLEAIRPVLLHGLKVLSGEITQHGPGTDGPASC"
BASE COUNT          250 a          574 c          561 g          225 t
ORIGIN      
        1 ccccggcgcg cgacggatcg gctcttcgag cacggctttc cacgtctcgc gatcggtgcg
       61 ttggtgtccg gccatgcgcc ggagcgcggg gcggacgatc tggtcgagaa ccaccagcga
      121 ggaaaccgga ttgcccggaa gtccgaagac cggctttccc tcgatgaacc cgaaagccag
      181 cggtttcccg ggacggatgg ccacccgcca gaagtccatt gtacccagtc gggacaggac
      241 aatcctggtg agatcgtgat cgcccaccga aacgcccccg gtcgagacca ccacgtcggc
      301 cgccgcgata gcccgtcgga agtgatcctc cagttccgac tccacgtcgg gagcgttccc
      361 tccgtcgagg accgtgtagc cgagcgaggc gagatgcgcg gccagcgtga atcgattcga
      421 atcccgtatt tgccccggcc ccggcgccga gtcggggggc accagctcat ctcccgatga
      481 gacgagcgcc acgaccggct ttccgaacac ccggacccgc gtcacgccga gggaggcgag
      541 gagccccacc tcggccgccc gcaccaggct gccggccgca agtgccaggc tccccctggg
      601 tacgctttcg cctgccagcc ggatgttggc cccggcacgc accggcttgt cgatcaggac
      661 gtgctgcccg tccggctgac tcgtctcgac gatcgcgacg gcatcggccc ccggcgggat
      721 cggcgcgccg gtcatgatgc ggatcgtttc gccggcggcc acgccacggg tgggcgcgcg
      781 cccggccggc aggtcatcga ccacgagaag gcgggccggc gactcggaag aggccgcggc
      841 cagatcggcg gcgcgcaccg cgaagccgtc catgctcgag ttggcgaacg ggggaagatc
      901 ttcgccggcc acgacatcct cggccaaaat gtgaccgagc gccgcggtga agctcgtctc
      961 gagcaccggc atcaccggta acgcggccag gatgcgttgc ttggcttcgg ggagtgtcag
     1021 cacgaggcgg gcccgtccgt gccgggaccg tgctgcgtga tctcgccgga aagcaccttg
     1081 agcccatgaa gcagtaccgg ccggatagcc tccaggcact cccgcacgcc cttcgggctg
     1141 ccggggaggt tcacgatgag ggtgccggcc cgcaggccgc acacgccgcg cgacaggggt
     1201 gcgagcggcg tcagacccgc cgtgtcgcga cgcatcagct cggcgaggcc gggggcctca
     1261 cgctcgatca cgtcgcgggt ggcttcgggc gtcacgtcgc gtcgcgagaa gccggtgccg
     1321 ccggtcgtga cgatcagggc gaaggtgccc gagtcggcca gtcgggccag ccgctccgag
     1381 accaccgcgc ggtcgtccgg aagaatttcc gtgcttgtga cctcgccgcc aagccgctcg
     1441 atggccgccc gggcttccgg tcccgagaca tcgggccgct cgccgcggga cgacgaatca
     1501 ctgacggtga tgatcgccgc ccggaacgtg gtcgtcgtca tccgcggggc cggcgtcgcg
     1561 gttcgcggac gtaacggccg ctcgcgccgc cgtccttcga aaccagctcg
//
LOCUS       DASVOD010000129         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1000779, whole genome shotgun sequence.
ACCESSION   DASVOD010000129 DASVOD010000000
VERSION     DASVOD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000779"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VF720_02600"
     CDS             <1..963
                     /locus_tag="VF720_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7878269.1"
                     /translation="GRTIILITHRLADVLGHTDAVTILRRGRDVAHRRTADTTYDELA
                     GMLVATGTGETSVAGLRAHAAATPVASPVDASSSHGPALLELRNVSLPASEHGAPLFD
                     VSLTVRAGEIVALVGVDGSGPSEVIRIAAGRLSPASGEVVLPTARGWIPENRHEEAIV
                     PAMTTAENLVLGRQNDARFGGKGWIDYARITTHAAQLLDRYDVRPAEPGRTVDTLSGG
                     NQQKLVVARETSDGPPLLVAAHPARGLDILATRRVHDELRAARGRGAAVLLMSTELDE
                     VRAVADRIVVFYAGRIVGELTAGRATDEALGRLMTGGQPTEPVA"
     gene            960..2012
                     /locus_tag="VF720_02605"
     CDS             960..2012
                     /locus_tag="VF720_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878270.1"
                     /translation="MKKLVTALAWPVGAALAGLVVAALLAGLAGEDPLRVLGILARSA
                     FGSSFGFGYTIYYATPLIFAGLAVALPYRAGLFNIGGEGQLLVGSAAVAAAGIGFASL
                     PSVVAIPLGIAAGALAGAAWGLLAGWLRARRGAHEVIVTILLNIVAGALVNWIILALL
                     KDPSSQAAESLPIGEGFRIESRLVGGTPANWTFLLGIALAVLAHYLITRTVFGFDIRA
                     TGQNPSAARAQGISEGRVLMLVMALAGGLAGLVGVNEVMGQAWRLKDSFSPGYGYTGI
                     AVALLARGRPLGVIPAALFFGALHRGAADLDIDTENVSRDLALVMQAIVILVVAVEAG
                     RLRRTRTTAAGPGGRA"
     gene            2009..>2569
                     /locus_tag="VF720_02610"
     CDS             2009..>2569
                     /locus_tag="VF720_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878271.1"
                     /translation="MNETLLLVTTLAASTIRLATPLIWAAMGGLLCERAGVVNIALEG
                     LLLTGAFVGAVAAHDTGSPWLGLAAGMAAGSLVGLLYAVATVRFRADQIVAGTAINIL
                     VVGLTAVVARALFGVTGSSPALPREARFAEIHVPALADIPVMGPVFFQHLPLVYLAPV
                     VALILAMVLRRTRAGLRLTAVGEAPDA"
BASE COUNT          317 a          887 c          941 g          424 t
ORIGIN      
        1 gggcggacga tcatcctcat cacgcatcgg ctggccgacg tgctcggcca caccgacgcg
       61 gtcacgatcc tccgtcgcgg ccgtgacgtg gcgcaccggc ggacggccga cacgacctat
      121 gacgagctgg ccgggatgct ggtggcgacg gggaccgggg agacgtcggt tgccgggttg
      181 cgtgcccacg ccgccgcgac gccggtggcg tcgccggtcg acgcctcgtc atcccacggc
      241 ccggccctcc tcgagttgcg caacgtgtcg cttccggctt cggagcacgg cgcgccgctc
      301 ttcgacgtgt cactcaccgt ccgggcgggc gaaatcgtgg cgctggtcgg cgtggacggc
      361 agcggcccct ccgaggtgat ccggatcgcc gccggtcgat tgagtcccgc gtcaggcgag
      421 gtggtgttgc cgacagcccg tggttggatc cccgagaatc gccacgagga ggcgatcgtg
      481 ccggcgatga cgaccgcgga gaatctcgtg ctcggccggc agaatgacgc gcgtttcggc
      541 gggaaggggt ggatcgacta cgcccggatc accacgcacg ccgcgcaact tctcgaccgc
      601 tacgacgtgc ggccggccga gcccggtcgg acagtggaca cgctctccgg gggaaaccag
      661 cagaagctgg tggtggctcg cgagacgtcc gacgggccgc cgctcctcgt ggccgcgcat
      721 ccggcgcgcg gactcgacat cctggccacg cgacgcgtgc atgacgaatt gcgcgccgcc
      781 cgcggacgcg gcgccgccgt gctcctcatg tcgacggaac tcgacgaggt gcgcgcggtg
      841 gccgaccgga tcgtcgtgtt ctatgccgga cggatcgtgg gagagctcac ggcgggccgg
      901 gccaccgatg aggcgctcgg ccgcctgatg acaggcggcc agccgacgga acccgtggcg
      961 tgaagaagct cgtcacggca cttgcgtggc cggttggcgc ggcgctggcc ggcctcgtcg
     1021 tagcggcact cctggccggc cttgccggcg aggatccgct ccgcgtgctc ggcatcctgg
     1081 cgcgatcggc gttcggctcg agcttcggct tcggctacac gatctactac gccacgccgc
     1141 ttatcttcgc gggactcgcg gtggcgctgc cgtaccgggc cggcctcttc aacatcggcg
     1201 gcgaggggca gctcctggtc ggctcggcgg cggtggcggc ggccggcatc ggcttcgcgt
     1261 cgcttccttc cgtggtggcc atcccgctcg ggatcgcggc cggtgctctg gcgggcgccg
     1321 cgtgggggct tctggccgga tggttgcgcg cccggagggg cgcgcatgag gtcatcgtca
     1381 cgatcctcct gaacatcgtg gcgggtgcgc tggtgaactg gatcatcctg gcgctgttga
     1441 aagacccgtc gagccaggcg gccgagtcgc ttccgatcgg cgaggggttc aggatcgaga
     1501 gtcggttggt cggcggcacg ccggccaact ggacattcct cctcggcatc gcgctggcgg
     1561 tgctcgctca ctatctcatc acgcgcaccg tcttcggctt cgacatacgg gccacggggc
     1621 agaatccgtc ggccgcccgc gcgcagggaa tttcggaagg acgcgtcctc atgctggtga
     1681 tggcgctggc cggcggcctg gccggcctcg tcggcgtgaa cgaggtgatg gggcaggcct
     1741 ggcgtctaaa agacagcttc tcgcccggct acggctacac gggaatcgcg gtggcgctgc
     1801 tggcgcgcgg caggccgctc ggcgtcattc ccgcggccct cttcttcggg gcgctgcacc
     1861 ggggtgccgc cgacctcgac atcgataccg agaacgtcag tcgcgatctc gcgctcgtga
     1921 tgcaggccat cgtcatcctg gtggtggccg tcgaggcggg gcggctgcgc cggacgcgga
     1981 ccacggcagc cgggccggga ggtcgtgcat gaacgagacg cttcttctcg tcaccacgct
     2041 ggccgcctcc acgatccgac tcgccacgcc cttgatctgg gcggcgatgg gcgggctcct
     2101 gtgcgagcgc gcgggcgtcg tgaacatcgc gctcgaaggg ctcctcctga ccggcgcctt
     2161 cgtgggcgcg gtggccgcgc acgacaccgg ctcgccctgg ctcggcctcg cggccggcat
     2221 ggcggccggg agcctggtgg gcctcctgta cgccgtcgcc accgtgcgtt tccgggccga
     2281 ccagatcgtg gccggaacgg ccatcaatat cctggtggtc gggctcacgg ccgtcgtcgc
     2341 gcgggcactg ttcggcgtga cgggatcgtc gccggcattg ccgcgcgagg cgcgcttcgc
     2401 ggagatccat gtgccggcgc tcgccgacat cccggtaatg ggtcctgttt tctttcagca
     2461 tctgccgctc gtctacctcg cgccggtcgt cgcgctgatc ctcgccatgg tgcttcgtcg
     2521 caccagggcg ggcctccggt tgaccgcggt cggcgaggca cccgacgcc
//
LOCUS       DASVOD010000130         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_269928, whole genome shotgun sequence.
ACCESSION   DASVOD010000130 DASVOD010000000
VERSION     DASVOD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269928"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1204)
                     /locus_tag="VF720_02615"
     CDS             complement(143..1204)
                     /locus_tag="VF720_02615"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7878272.1"
                     /translation="MTIGKNATPSAIVLDTGGGTSAFDPEVVTAAGIKLGQPIRAMGA
                     GGETREAWLLGDVSYSLPGVTLEGQSITAISFRHLEETTGRRMDGVLGSDFLRQFVVI
                     IDYEGRKLHLHDPKSYQYEGDGHPLPLKFGGPIPTVKATLNGTIDAEFMVDTGARMGV
                     KASRMFVERAKLDDAANAVIEGGPGYGIGGATAQGVARFDKFSVAGFDLPSPLVFLSR
                     DSAGVFGKGESDGVLGGDVWRRFTVILDYPNKMMYLEPNASFNDLFEYDMSGLALRMD
                     NESKGGFLVSAVLSNSPAEAAGMQVGDRIEAVDDKPVASMVLEDIRESFREAGRTVQV
                     TRRRGDAVSVVTLTLKRLI"
     gene            complement(1485..2180)
                     /locus_tag="VF720_02620"
     CDS             complement(1485..2180)
                     /locus_tag="VF720_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HEX7878273.1"
                     /translation="MKTPAAPSPAELEQLDTFLKSKRLGPEALRTHGIDGLVTAIVIG
                     PDMVMPSQWMPRVWDYRDLKFKDEHVAERIIGFVMGLYQRVASLFADDTSDRFEPLYV
                     RLSEGKEREKAIHDWCRGFMAGLALHPSMKDKESREPLLEFIWPMAAMTGGGKLATPA
                     VIETIVPSVRSLHAIWLKGRRAVVPGPARGMGAARGVGQPAPAAPGRNDPCPCGSGLK
                     FKKCCGGPGGTVH"
BASE COUNT          413 a          855 c          684 g          409 t
ORIGIN      
        1 caggttcgtg ctcgactgtg cagtctcatc cggggctccc ctctgctggt ggtcggtagg
       61 cctaagcacc accaacttcc aacagaagag ccccgggtga ctactccccc cgggcagtga
      121 acaacctatt gagaaatcac agctagatga gccgtttcag cgtcagcgtc acgacgctga
      181 ccgcatcccc gcgccgccgc gtgacctgca ccgtgcgccc cgcctcgcga aacgactcgc
      241 ggatgtcttc cagcaccatc gacgccaccg gcttgtcatc caccgcctcg atgcgatcgc
      301 cgacctgcat gccggccgct tcggccggcg agttgctgag caccgcgctc acgaggaagc
      361 cccccttgga ctcgttgtcc atccgcaagg ccagcccgct catgtcgtac tcgaagaggt
      421 cgttgaacga ggcattcggc tcgagataca tcatcttgtt cggataatcg aggatgacgg
      481 taaagcgacg ccatacgtcg ccccccagca caccgtcgct ctctcccttg ccgaacacgc
      541 ccgccgaatc ccggctcagg aagacgagcg gcgacggcaa atcgaacccc gccacgctga
      601 acttgtcgaa ccgcgccacc ccctgcgcgg tggcgccgcc gatcccgtac ccgggcccgc
      661 cctcgatgac cgcgttcgcc gcgtcgtcca gcttggcccg ttccacgaac atccggctgg
      721 ccttcacgcc catgcgcgcg ccggtatcga ccatgaactc cgcgtcgatc gtgccgttca
      781 acgtggcctt cacggtcggg atcggaccgc cgaatttcag cggcagcgga tgcccatccc
      841 cctcgtactg gtacgacttc ggatcgtgga gatgcagttt ccttccttcg tagtcgatga
      901 tgacgacgaa ctgccgaagg aaatcggacc ccaggacgcc atccatgcgg cggccggtcg
      961 tctcctccag atgccggaac gagatggccg tgatcgactg cccctcgagc gtgacgcccg
     1021 gaagcgagta actcacatcg ccaagcagcc acgcctcccg cgtctctccc cccgcgccca
     1081 ttgcccggat cggctgcccg agtttgatcc ccgccgccgt cacgacctcc ggatcgaacg
     1141 ccgaggtgcc cccgccggtg tcgagcacga tcgccgaagg cgtggcgttc ttgccgatgg
     1201 tcacgggcac gtagatgtga ttgctgctca gttcgatcgg aatctcgaca gccgacgtgc
     1261 cgttcgcgaa cttcgccgga ggccccttcg gcggctggta atgctcgaca tgggtgccat
     1321 ggtggccggc gctgccgaac gcgggatccg cggcgacggg cctgccggcg gtggggggcc
     1381 cggcggccgc gcagggcgtg gatcatcctt ggcatgttgg tttcctccga ccggtgacac
     1441 cttgaggccc caccgggtcg cagagaatcg tatgggagcg gccttcaatg aaccgtgccg
     1501 cccggccccc cgcagcactt cttgaacttg agcccgctcc cgcacgggca cgggtcattc
     1561 cggccagggg ccgcgggcgc cggctgtcca acacctcgcg cggcgcccat gccccgagcc
     1621 ggccccggca ccaccgcccg ccgccccttc agccagatcg catgaaggct gcgaacactc
     1681 gggacgatcg tctcgatgac ggccggagtg gccagcttgc ccccgcccgt catcgccgcc
     1741 atcggccaga tgaactcgag cagcggctcg cggctctcct tgtccttcat gctcgggtgc
     1801 agcgcgaggc cggccatgaa tccccggcac cagtcgtgga tcgccttctc gcgttccttc
     1861 ccctcggaaa gccgaacata gagcggctcg aaccgatccg aagtgtcatc cgcaaacagg
     1921 ctcgccaccc gctggtacag ccccatgacg aacccgatga tccgctcggc cacatgctcg
     1981 tccttgaact tcaagtcccg ataatcccac acgcgcggca tccactggct cggcatcacc
     2041 atgtccggcc cgatcacgat ggccgtaacc aggccatcaa taccatgcgt gcgtaacgct
     2101 tccggcccaa gccgcttcga cttcaggaag gtgtcgagct gctcgagctc agcaggggag
     2161 ggggcggcgg gtgtcttcat tccaaaggac tatagtggat tgcaatcatc acgccagcat
     2221 tcgtaagaac cgccaactcc atgccgccgg cccgctcacg cgatttccaa ctgattcacg
     2281 gaactcggcc cgaaccgagt tcgccaattg tttggaatat gaacgactgt ggctgtttcg
     2341 atgaagaagg gttgcccccc g
//
LOCUS       DASVOD010000131        11445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1000902, whole genome shotgun sequence.
ACCESSION   DASVOD010000131 DASVOD010000000
VERSION     DASVOD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11445
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000902"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VF720_02625"
     CDS             complement(<1..540)
                     /locus_tag="VF720_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7878274.1"
                     /translation="MTRFLARGAALLIGLFLGIVALELGLRLIGWSSPRLVEHDPDRG
                     KAYRPGIHWHQSEEGEADVVINRWGFRDRDWTVEKPPGTVRVALLGDSFVDGLQVPVD
                     STWARVAERRLNACRAFGDSTVEVMNFGVSGYGTAQELQTLRHEVLRFHPDVVVLSFL
                     PGNDVRDNGLLLRRDPGRPY"
     gene            complement(537..1814)
                     /locus_tag="VF720_02630"
     CDS             complement(537..1814)
                     /locus_tag="VF720_02630"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7878275.1"
                     /translation="MAAQPVVITGMGAASPIGMGVAAFREGLLGARSGTRAVTAFDAS
                     DLPCRVAADVTEPLEPLLTGIVEGKLVRNLSRTTMMALIAGAEALGSARWPVKNSDDA
                     AGAGVLGVWAGTGGAGVGWAEAQYEIYFRDGWRRTSPWGIVAGLPGMLSSDLSSAFGA
                     TGPSHVLSTGCTSSSDAISYATLAVAMGWCDAALAGGAEAPLTRGIFSNFCRMQAMGT
                     SFNDRPEAASRPYDRDRDGFILGEGAWFVVLETAEAAARRGVVPLARLAGTFSTCDAH
                     HRIRNHPSGAETARAVRSALAAAGVDPGRIGYVNLHGSATPLNDRTETLALTSVFGER
                     TPAIPMSALKSQIGHPQGASGAAGLVATLLGMAGGFVPATINRDHPDPDCGLDVVPRE
                     PREHRFEAAVVNAISFGSKNTALVITTPDFETE"
     gene            complement(1862..3094)
                     /locus_tag="VF720_02635"
     CDS             complement(1862..3094)
                     /locus_tag="VF720_02635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF003015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7878276.1"
                     /translation="MEAANGRPTRWIGHVDMDSFFASVEIHDDPSLAGKPVVVGGPPE
                     KRGVVAAASYESRVFGIHSAMPMARALQLCPHLVRVSPRGGRYKDVSDQVFDILRGFS
                     PLLEPLSLDEAFLDLTGSERLLGAPEAIGGEIRRRIRDVTGLTASVGIAPNKFVAKLA
                     SDHRKPDGLTVVPHAEAVTFVQELPIGRMWGVGDRTGEEMARLGIRTIGDFAALGPAR
                     VKQLFGIGALKMHELSWARDDRPVVPDQEPQSISHEITFARNQSSHDVLKGVLLGLCE
                     QVATRLRRHRFLGRIIVLKLRFADFRTITRRQTLGHHTDDAGEIFETAEALLVEARTS
                     SPLSIRLIGIGASGLVEPDQLPLDLFAADPVDARTNALNATMDRLQERFGREAVRRAR
                     SVLSDRPESTESTLERRD"
     gene            complement(3107..4321)
                     /locus_tag="VF720_02640"
     CDS             complement(3107..4321)
                     /locus_tag="VF720_02640"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEX7878277.1"
                     /translation="MGTDYFRLHPWIGILSNLPVVAGVAAMLLLLLPLFRRRLAEQPV
                     AWIGALFVVTRLVFLGLVVGTMGHVSLDLTTYFEAQGRAVLSGALPYRDFACSYAPLF
                     PYLMAPAVMTPWPTLAIFVTFIAFDLATVVAVAKGADDRDQAMRAAWLYTAAPISWYF
                     LVRYGQDEALAAALLAWAAVLLRNGGGRSAGFVLGLGFALTKFTFGLAMPPFALAARS
                     RRQLALGLGPPILIVFGVALLAGLPVWRPLFGESVELGFGPSLWRLPVLFTPLVLGRW
                     AGLVLLLGLAVAWWWCAKLPKPRRLVPGLVVTGCVFLLLSPKVLPMYLTPFGPAVAWW
                     LAGRADRGDLMLVGLLNLLLGTWWYVDAGGVQGMFGPLVQALAVAVTAAVPVLLALLI
                     ARVVRTEPRETA"
     gene            4390..6042
                     /locus_tag="VF720_02645"
     CDS             4390..6042
                     /locus_tag="VF720_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878278.1"
                     /translation="MSEPARTGPPTIDWMFLSAALSLAILVPLLGIPVYFRTDDVHWL
                     GWAATHPDPLSAFDPAENLFGYYRPLPTLLWWVLFRFFGFQPLGYQIVLAALGVGAMV
                     PLARIGRRLAGGSWGALAAVGLFHAAFVSILYFYYWFSAITFALELLFLALALDALSD
                     GKRIPGRPVHFAIFALLAGLAKQPALLVIPAVTITFLLLESGSPRRRFLWGAGVAAVA
                     LLLMLVTPFTAQRPEALGHLAGDERTRYLLDRLRFYAGVLLRGPAGMLGAAGALVALA
                     SWRGERRPSSLVLYGVLPGILAGVSLARLDPALAIGPWLALLVAASVVVPASRPWLAG
                     FLVPAAALLLVDFHVSTYLIEPGLALTPALLLWWRPLLEAPAVRIESKLRRSALVIAC
                     VAPLVSLLAAYLLHDRVHPIKAMRDVRKVFRETVEQVIDEAPAGTIIGYLSYEELGET
                     YADIRRRPLEQRVERHKTMNGAQLGKFLRLRGRADLSVVPVAEAWAASDSAGAPVWLL
                     AVTAAERAELAARSGAREVAWTRQGSAEAALFRVGSPNSVKK"
     gene            6181..8103
                     /locus_tag="VF720_02650"
     CDS             6181..8103
                     /locus_tag="VF720_02650"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX7878279.1"
                     /translation="MPLLRPLKRRGLAAFLAALVLTLGAPRAEARFPGPPTANIYVRN
                     YYEPGDAELLSRYDLLALDADTPPSVIQEIRSYGTGTKILAFIPANGTYEAALNFPDG
                     SIWREIYEAAETHGWWMKDTHGGHINDHGAKFTTNITTVCPPNAQGKTILDWFPTWVV
                     NTLLVNNQTNWDGVFLDDCWVGIYWVSYDTQLNPYPIDANNDGVADPQAQLDEWYKAG
                     TDTLVTRIRRQLPPDMIVMGNGQNHFYSLNGAMIENFPYTGYADAGCPYNYSWTWDMF
                     GPWGYTNNTTNYSPNPARYNMINSKWIWGDRYNPTITADFERFKDFTLAASMLRDGYY
                     SLDWYDLPNTKKSHHSIWWQPEYDIPFGNPLGPAYQLTRAGVTIWRRDFDGGSVVVNP
                     NAMSFGPSIPDSLPIVPAWEAMLIKDGEFYFPDVTPPARVTDLKLTRVWADSVELEWH
                     NSGDDGMTGQAASLVIRRSSAGMITDANFTQGVRLQPAVSPEGPGTVQRAVFRGLVPG
                     TQYWFALRHIDDSGNAAPVSNNASAVAGGNTSGIPAPRPTIPALGPASPNPFNGSIAA
                     TILAPEAGTPVRLTVLDAAGRQVRTILDGPHPGGSMVADWDGRDESGASSPSGVYFLV
                     LTSGATTDVRKIVRSR"
     gene            complement(8118..9926)
                     /locus_tag="VF720_02655"
     CDS             complement(8118..9926)
                     /locus_tag="VF720_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7878280.1"
                     /translation="MRDVRSLVSLVVMALVILAARGSGAAEPPAWWVEQDGSTAGVPW
                     QAVTYRVDGLRVKGFLFDAAIPGRRPVVVFNHGGVSGVSADMMRRSADLARGGYLVFT
                     PTYRGEGGSEGRIEVAAGEVNDVLAAVHFLREHPRADGARVALVGSSHGALISVLAAA
                     RDSKVTAVAAACGVMDVHAWYRYLVDNGFDVSDSLSVAVYGTGPESRPEAFRDRSAIR
                     VAGKIHQPLLLMQGMKDRTVPPDQVWRMSAAMERAGSGRPEVRTYPLLGHAFWFWDRA
                     HHSEAEVVQAEESWSELMSFLERNLMPDSHTTGAAPRAGAATGGGATTDDQAWRSELA
                     EWQTRRAARLTADDGWLTVAGLFWLEPGLNRMGTAEDNAIRLPEGSAPPRAGSFLLEN
                     GVVSVESLPGVPLMNQGKPIERMTLRADADGSPDTLALGDLRMIVIRRSKGLAIRLRD
                     LSAEARRNFAGIECFPPDPAWRIEARWVPYDPPKPVEIPNIIGSIDTMMAPGRAEFEK
                     DGRAYSLEPVLEEGDTDLFYIFKDGTSGKTTYPPGRFLYSGAPVDGKVTLDFNRAYNP
                     PCAFTAWATCPLPPRQNELSLDVTAGEKLYGEASGH"
     gene            complement(9919..10671)
                     /locus_tag="VF720_02660"
     CDS             complement(9919..10671)
                     /locus_tag="VF720_02660"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX7878281.1"
                     /translation="MPSTIRINVFLARAGVASRRGADRLVETGQVRVNGTVVTLLGTA
                     IDPARDRVEVAGQVVGSPQSLHYMVMNKPTGYVTTARDPEGRPTVLDLAGPTGARLYP
                     VGRLDMDSEGLLFLMNDGRLAFRLTHPKYDVPKTYRAWTSAPPSAGAIRQLREGLELE
                     DGPTRPAEVIERGRMPAGAGVAGPAIDITLKEGRNRQVRRMLEAVGFPVVRLRRIRFG
                     PIELGNLSPSEHRPLTRAEVNDLRRLVRLPHA"
     gene            11020..>11445
                     /locus_tag="VF720_02665"
     CDS             11020..>11445
                     /locus_tag="VF720_02665"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEX7878282.1"
                     /translation="MQIKHQGLGSLLVDEGMLTQDQLGKALQEQTSTHERLGAILLRL
                     GYVREPDLVRVLARQLGVSTFDPTIHKVMPDAVSLVPLDMAERYELVPVVRENGRLTV
                     AMADPLNLMALDDLRYRTQTEPIVMIGLADDIAVARRKHY"
BASE COUNT         1864 a         4028 c         3719 g         1834 t
ORIGIN      
        1 gtaggggcga cccgggtcgc gccgcagcag gaggccgttg tcgcgcacat cgtttccggg
       61 aagaaacgac aggacgacca cgtccggatg gaaccggagc acctcgtggc gcagggtctg
      121 cagctcctgg gcggtgccgt aacccgacac gccgaagttc atcacctcca cggtcgaatc
      181 gccgaaggca cggcaggcgt tgagccgccg ctcggccacc cgcgcccacg ttgagtcgac
      241 gggcacctgg agtccgtcca cgaagctgtc gccgagaagc gccacccgca cggtgcccgg
      301 tggtttctcg acggtccagt cccggtcgcg gaaaccccat cggttgatga ccacgtccgc
      361 ctcgccttcc tccgactgat gccagtggat gccgggccgg tacgccttgc cgcgatccgg
      421 atcgtgctcg accagcctgg ggctcgacca cccgatcagg cgcaggccga gttccagcgc
      481 cacgattccc aggaagagac cgatgagcag cgcggcgccc cgcgcgagga agcgggtcat
      541 tccgtctcga agtccggtgt cgtgatcacg agcgcggtgt tcttcgatcc gaacgagatg
      601 gcattcacca cggccgcctc gaagcggtgc tcccgcggct cccgcggcac gacgtccaga
      661 ccgcaatcgg ggtcggggtg gtcgcggttg atcgtggccg gcacgaatcc gccggccatg
      721 cccagcagcg tggccacgag gcccgcggcg cccgaggctc cctgcgggtg gccgatctgc
      781 gatttcagcg cgctcatcgg gatggcgggc gttctctcgc cgaataccga ggtgagcgcc
      841 agcgtttcgg tccggtcatt gagcggcgtg gcgctgccat gcaggttcac gtacccgatc
      901 cggccgggat cgacaccggc agccgccagc gccgagcgga cggcccgggc cgtttcggcg
      961 ccggacggat ggttccggat gcggtggtgc gcatcgcagg tggaaaaggt gccggccagc
     1021 cgggcgagcg gcacgacgcc ccgccgcgcg gctgcctcgg ccgtttccag gacaacgaac
     1081 caggccccct cgccgaggat gaacccgtct cgatcgcgat cgtacggacg gctggcggct
     1141 tccgggcggt cgttgaacga cgtgcccatc gcctgcatcc ggcagaagtt ggaaaagatg
     1201 ccacgggtca ggggcgcttc cgcgccgccg gccagcgcgg catcgcacca acccatggcc
     1261 acggcgagtg tggcgtagga gatggcatcg ctggacgacg tacagccggt ggagagcacg
     1321 tgcgagggtc ccgtggcccc gaacgccgag gacaggtcgc tcgacagcat tcccggcagc
     1381 ccggccacga ttccccaggg actcgtccgc cgccacccgt cccggaaata gatctcgtac
     1441 tgcgcctcgg cccaccccac gccggcgccg cccgtgccgg cccacacgcc gagcactccg
     1501 gcgcccgcgg catcatcgga attcttcacc ggccagcgtg ccgagcccaa tgcctcggcg
     1561 cccgcgatca acgccatcat cgtcgtccgc gaaaggttgc gcacgagctt cccctcgacg
     1621 attcccgtga ggagcggctc cagcggttcg gtcacgtcgg cggccacgcg gcacggcagg
     1681 tcggaagcat cgaaggccgt gaccgcgcgc gtgccgctcc gcgcgcccag caagccttcg
     1741 cggaacgccg ccacgcccat gccgatgggc gaggccgcgc ccatgccggt gatgaccacc
     1801 ggctgcgccg ccatcgggga cctcgttgcg gcatcgaccc tgccggaacc gcgcgtagtt
     1861 gtcagtctcg ccgctccagc gtcgattcgg tcgattcggg ccgatcgctc aggacgctgc
     1921 gcgcgcgccg gacggcctcg cggccgaaac gctcctgcag ccggtccatt gtggcgttca
     1981 gggcgttcgt ccgtgcgtcg accggatcgg ccgcgaatag atcgaggggc agctggtcgg
     2041 gttccaccag ccccgaggcg ccgatgccga tcaaccggat ggagaggggc gagctggtgc
     2101 gcgcctccac cagcagcgcc tccgcggttt cgaagatctc ccccgcgtcg tcggtgtggt
     2161 gccccagcgt ctgccggcgg gtgatcgtgc ggaagtcggc gaagcggagt ttcaggacga
     2221 tgattcgccc gaggaagcga tgccgccgca accgcgtggc cacctgctcg cacaggccga
     2281 gcagcacccc cttcaggacg tcgtgcgacg actggttgcg tgcgaaggtg atttcgtggc
     2341 tgatcgactg cggctcctgg tccggcacga ccggtcggtc gtcgcgggcc caggatagtt
     2401 catgcatctt gagcgccccg atgccgaaga gctgcttcac gcgggccggc ccgagtgccg
     2461 cgaaatcgcc gatggtcctg attcccaggc gggccatctc ttctccggtc cggtccccga
     2521 cgccccacat cctgccgatc ggcaactcct gcacgaacgt cacggcctcg gcgtgcggca
     2581 cgacggtgag cccgtccggc ttgcggtggt ccgacgccag cttggccacg aacttgttcg
     2641 gtgcgatccc taccgaggcg gtgagccccg tcacgtccct gatgcggcga cggatctccc
     2701 caccgatcgc ctccggggcg ccgagcagcc gctcgctgcc ggtcaggtcc aggaaggcct
     2761 cgtcgaggga aagaggttcg agcagcggcg agaagccgcg caggatatcg aacacctggt
     2821 ccgacacgtc cttgtatcga ccgccgcgcg gcgacacgcg cacgaggtgg ggacagagct
     2881 ggagggcacg ggccatcggc atggcggagt gaatgccgaa aacccgtgat tcgtacgagg
     2941 cggcggccac cacgccccgt ttttcgggag gaccgcccac caccacgggc ttgccggcca
     3001 gcgaggggtc gtcatggatc tccaccgagg cgaagaacga atccatgtcg acgtggccga
     3061 tccagcgggt cggccgcccg ttggccgctt ccatcggcga agtctatcac gccgtctcgc
     3121 gcggttccgt tcgcaccacg cgggcgatga ggagggcaag gagcaccgga acggcggccg
     3181 tcaccgccac cgccagtgcc tgcaccagcg ggccgaacat tccctgcacg ccgcccgcat
     3241 ccacgtacca ccaggtgccg agcagcaggt tcaggaggcc caccagcatc aggtcgcccc
     3301 gatccgcgcg cccggccagc caccaggcca ccgccggccc gaatggagtc aggtacatcg
     3361 ggaggacctt gggggagagg agcaggaaga cgcacccggt caccacgagg cccggaacga
     3421 ggcggcgcgg cttcggcagc tttgcgcacc accaccatgc gacggccagg ccgagcagca
     3481 gcacgaggcc ggcccaccgc ccgagcacga ggggggtgaa gagcaccggc agtcgccaga
     3541 ggctcgggcc gaatcccagc tccaccgact caccgaacag gggacgccac accggcaggc
     3601 cggcgaggag cgccactccg aatacgatga ggatcggcgg gccgagcccc agcgccagct
     3661 gccgtcgcga tcgtgccgcc agggcgaacg gcggcatcgc gaggccgaac gtgaatttgg
     3721 tgagggcgaa gcccagcccg agcacgaatc cggccgatct cccgcccccg ttccgcagca
     3781 acacggccgc ccaggccagg agcgccgcgg ccagcgcctc gtcctgcccg taacgcacca
     3841 ggaagtacca ggagatcggc gccgccgtgt acaaccaggc ggcccgcatc gcctggtccc
     3901 gatcgtcggc acccttcgcc accgccacca cggtggcgag gtcgaaggcg atgaaggtca
     3961 cgaagatggc gagtgtcggc catggcgtca tcacggccgg cgccatgaga tagggaaaaa
     4021 ggggtgcgta cgagcaggcg aagtcccggt agggaagcgc acccgaaagc acggctcgtc
     4081 cctgcgcctc gaagtaggtg gtgagatcga ggctcacgtg tcccatcgtt cccaccacga
     4141 gtccgaggaa gacgaggcgc gtgacgacga agagggcccc gatccaggcc accggctgct
     4201 ccgccagcct tcgacggaag agggggagca ggaggaggag catggccgcc acaccggcca
     4261 cgaccgggag attggacagg atgccgatcc acgggtgcag ccggaagtag tcggttccca
     4321 tcgggtggcc actctatcag gcccgggcaa ctcctgccgt tcgcgggggc ggcctgctag
     4381 agtgccgccg tgagcgagcc cgcccgaacc ggcccaccga cgatcgactg gatgttcctc
     4441 tcggcggccc tctcactggc tattctcgtg ccgctgctcg ggatcccggt ctacttccgc
     4501 accgacgatg tccactggct gggctgggcc gccactcatc ccgatccgct gtcggccttc
     4561 gatcccgccg agaacctctt cggttattac cggccactcc ccacgcttct ctggtgggtg
     4621 ctcttccgct tcttcggttt ccagccgctc gggtaccaga tcgtcctggc cgccctcggc
     4681 gtgggtgcca tggtgccgct ggcccggatc gggcgccggc tggccggcgg gtcgtggggc
     4741 gcgctggccg cggtcggcct gttccatgcc gccttcgtgt cgatcctcta cttctattac
     4801 tggttcagcg ccatcacctt tgcgctggag ctcctcttcc ttgcgcttgc gctcgacgcc
     4861 ttgagcgacg gcaaacggat ccccgggcgt cccgtccatt tcgccatctt cgccctcctg
     4921 gcgggcctgg ccaagcaacc ggcgctgctc gtcattccgg ccgtcaccat caccttcctt
     4981 ctcctcgagt cgggttcgcc acgtcggcgc ttcctgtggg gcgcaggtgt cgcggcagtg
     5041 gccctcctgt tgatgctggt cacgccgttc acggcgcaac gtcccgaggc gctcggtcac
     5101 ctggcaggcg acgaacgcac acgctatctc ctcgaccggc tccggttcta cgcgggcgtg
     5161 ctgctgcggg ggccggcggg catgctcggc gcggccggtg cactggtggc cctggcctca
     5221 tggcgcggcg aacggcgccc gtcgtctctc gtcctttacg gcgtgcttcc cgggattctt
     5281 gcgggcgtct cactggcccg cctcgatccc gcgctggcca tcgggccgtg gcttgctctt
     5341 ctcgtcgcgg catccgtcgt cgtccccgcc tcgcgcccct ggctggccgg attcctcgtg
     5401 ccggccgccg cgctcctcct ggtcgacttc catgtttcca cctacctgat cgagccgggg
     5461 ctggcgctca caccggcgct cctgttgtgg tggcgcccgc tcctcgaggc acccgcggtg
     5521 cggatcgaaa gcaagctccg gcgctccgcc ctcgtcatcg cctgcgttgc gccgctcgtg
     5581 tcactcctcg cggcgtacct tcttcacgat cgggtgcacc cgatcaaggc catgcgcgac
     5641 gtgcggaagg tcttccggga gacggtcgag caggtgatcg acgaggcccc ggcaggaacc
     5701 atcattgggt acctgagcta cgaggagctc ggggaaacgt acgccgatat ccgccgccgc
     5761 ccgctcgaac agcgggtgga gcggcacaag accatgaatg gggcccagct cgggaagttt
     5821 ttacgtctgc gtggtcgtgc cgacctgagt gtcgtacccg ttgccgaggc ctgggccgca
     5881 tccgattccg cgggtgctcc cgtatggctc ctggcggtta cggcggccga acgggccgag
     5941 ctggccgccc ggagcggggc ccgggaggtg gcttggaccc gccagggaag cgccgaggct
     6001 gcccttttcc gggtgggctc acccaattcc gtgaaaaaat agggtcgaaa tcgacgtggc
     6061 cgccgcccct ctcgtcgcgt aaactctctc ccattgccaa ccggccgtat ccccgacaga
     6121 tgccagcgct ccggcgcgct tcgtaacgaa agggcgaacc tgcatccgat gctcgcctgc
     6181 atgcccttgt tgcgcccgtt gaaacggcgc ggcctcgcgg ccttcctcgc cgcgctggtg
     6241 ctcactctcg gagctccccg ggcggaggcc cgttttcccg ggccccccac ggcaaatatc
     6301 tacgtccgga actactacga accgggggat gcggagctcc tctcccgcta cgacctcctg
     6361 gccctcgacg ccgacacgcc tccctcggtc atccaggaga tccgctctta tggaacgggc
     6421 accaagatcc tggccttcat tcccgccaac ggcacctatg aggccgccct caattttccc
     6481 gacgggagca tctggcgaga gatatacgaa gccgccgaga cccatggctg gtggatgaag
     6541 gacacccatg gcggtcacat caacgaccac ggcgcgaagt tcaccaccaa catcaccact
     6601 gtctgtccgc ccaacgccca ggggaagacg atcctcgact ggttccccac ctgggtcgtg
     6661 aacacgctcc tggtcaacaa ccagacgaac tgggacggcg tgttcctcga cgattgctgg
     6721 gtgggcatct actgggtgag ctatgacacg cagctcaacc cgtaccccat cgatgccaac
     6781 aacgacgggg tggccgatcc gcaggcccag ctcgatgagt ggtacaaggc gggcaccgac
     6841 acgctcgtca cccgcatccg ccggcagctc ccgcccgaca tgatcgtgat gggcaacggc
     6901 cagaaccatt tctactcgct caacggggcg atgatcgaga acttccccta caccggttac
     6961 gccgacgccg gctgtcccta caactattca tggacatggg acatgttcgg accctggggc
     7021 tacaccaaca acaccaccaa ctattcgccc aatcccgccc gttacaacat gatcaattcg
     7081 aaatggatct ggggcgaccg ctacaacccc accatcaccg ccgatttcga gcgtttcaag
     7141 gacttcacgc tggccgcctc gatgttgcgg gacggctatt actcgctcga ctggtatgac
     7201 ctgcccaaca ccaagaaatc gcaccactcg atctggtggc agccggagta cgacatcccc
     7261 ttcggcaatc cgcttggccc ggcgtaccag ctcacccggg ccggcgtcac gatctggcgt
     7321 cgcgacttcg acggcggttc ggtcgtggtg aatcccaatg ccatgagttt cggcccgtcc
     7381 attcccgaca gtctcccgat cgtccccgcc tgggaggcga tgctcatcaa ggatggagag
     7441 ttctactttc ccgacgtgac tccgcccgcc cgtgtcaccg acctcaagct gacgcgcgtc
     7501 tgggccgact cggtcgagct ggaatggcac aactccggcg atgacggaat gacgggccag
     7561 gcggcctccc tggtgatccg gcgctcgtcg gccggaatga tcaccgacgc caacttcacc
     7621 cagggggtga gactccagcc cgcagtctct ccggaagggc cgggcacggt ccagcgcgcc
     7681 gttttccgcg gcctcgtgcc gggcacgcag tactggttcg cgcttcgaca catcgatgac
     7741 tcgggcaatg cggccccggt ttcgaacaac gcttcggccg tggccggtgg gaacacgagc
     7801 ggcatcccgg cgccgcgtcc cacgattccc gcgctcggac ccgcctcgcc gaacccgttc
     7861 aacggttcga tcgccgccac gatcctggct cccgaggccg gcacaccggt ccggctgacc
     7921 gtgctcgatg cggcgggacg ccaggtgcgg acgatcctcg acggtccgca ccccggtgga
     7981 tcgatggtgg ccgactggga tggccgcgac gaatcgggag cgtcatcccc ctcgggcgtc
     8041 tacttcctcg tattgacgag cggcgccacc accgacgtcc ggaagatcgt tcgaagccgc
     8101 tgaccgtccg aaccggctca gtgccccgaa gcctccccgt acagcttctc cccggcggtc
     8161 acgtcgagtg acagttcgtt ctggcgcggc ggcagcgggc aggtggccca cgccgtgaag
     8221 gcgcagggcg ggttgtaggc gcggttgaag tcgagcgtca ccttgccgtc gaccggtgct
     8281 cccgagtaga ggaatcgccc cggcggatag gtggtcttgc cgctcgtccc gtccttgaag
     8341 atgtagaaga ggtcggtgtc cccttcctcg agcaccggtt caaggctgta cgcgcggcca
     8401 tccttctcga attcggcgcg gccgggcgcc atcatggtgt cgatgctgcc gatgatgttc
     8461 gggatctcga ccggcttcgg gggatcgtag ggaacccagc gggcctcgat gcgccaggcg
     8521 ggatcgggag gaaagcactc gatccccgca aagttgcgcc gcgcctccgc cgacaggtcg
     8581 cgcagccgga tggccagtcc tttcgaacgg cggatcacga tcatccggag atcaccgagc
     8641 gcgagcgtgt cgggtgagcc gtcggcatcg gctctcaggg tcatccgctc aatcggcttc
     8701 ccctggttca tcaggggaac gcccggaagg ctttccaccg acacgacccc gttctccagc
     8761 aggaagctgc cggcccgggg cggcgccgat ccttccggca accggatggc gttgtcttcg
     8821 gccgttccca tccggttgag acccggctcc agccagaaga ggccggccac ggtgagccag
     8881 ccgtcatcgg cggtcagtcg ggcggcgcgc cgggtctgcc attcggcaag ctcggaccgc
     8941 caggcctggt cgtcggtcgt ggcaccgccc cccgtggccg cgccggcgcg cggggcggca
     9001 cccgtagtgt gggaatccgg catcaggttc ctctcgagga agctcatgag ctcgctccag
     9061 gactcttcgg cctgcacgac ctcggcttcg gaatggtgcg cgcggtccca gaaccagaag
     9121 gcgtggccga gcaggggata ggttctcact tcggggcgtc cggaccccgc ccgctccatt
     9181 gcggccgaca tccgccacac ctggtcgggt ggcacggtcc tgtccttcat tccctgcatg
     9241 aggaggaggg gctgatggat cttgccggcc actcggatgg cgctgcgatc gcgaaaggcc
     9301 tcggggcggc tttcgggacc ggtgccgtac accgccacgg aaagcgagtc ggaaacgtcg
     9361 aagccgttgt ccacgagata gcgataccag gcgtgcacat ccatcacgcc gcacgccgcc
     9421 gccaccgccg tcaccttcga gtcgcgggcg gccgccaata cggagatcag ggcgccatgg
     9481 ctgctcccca cgagcgccac gcgcgcgccg tccgcccgcg gatgctcgcg aaggaaatgg
     9541 acggcggcca ggacgtcgtt cacctcgccg gccgccacct cgatccgtcc ttcgctccct
     9601 ccctccccgc gatacgtcgg cgtgaagacc aggtagcccc cccgcgcaag gtcggccgag
     9661 cggcgcatca tgtccgccga aacgccgctc acgccgccgt ggttgaagac caccacggga
     9721 cggcgaccgg ggatcgccgc gtcgaagagg aatcccttca cccggaggcc gtccacccgg
     9781 taggtcacgg cctgccatgg gacgccggcc gtggacccgt cctgctcgac ccaccaggca
     9841 ggcggctcgg cggcgccgga tccgcgcgcc gcgaggatta cgagggccat gacaaccagg
     9901 gaaaccagcg agcggacgtc acgcatgcgg gagcctcacg agtcggcgga gatcattcac
     9961 ctccgcgcgg gtaaggggac ggtgttcact cggggagagg ttccccagtt cgatcgggcc
    10021 gaaacgaatc cgtcgcaggc gaacgacggg aaagcccacc gcctcgagca tccggcggac
    10081 ctgccggttg cgcccctcct tgagcgtgat gtcgatggcc ggcccggcga ctcccgcccc
    10141 cgcgggcatg cgtccccttt cgatcacctc ggccggtcgg gtggggccgt cctcgagttc
    10201 gagaccttcc cgcagttgcc ggattgcgcc ggcggacggc ggggccgatg tccacgcccg
    10261 gtaggttttc ggcacgtcat acttcggatg ggtgagccga aacgcaagcc gcccatcgtt
    10321 catcaggaac aggagcccct cggagtccat gtccagccgg cccacggggt agagccgagc
    10381 tccggttggt ccggccaggt cgagcacggt agggcgcccc tccgggtccc gcgccgtggt
    10441 cacgtacccg gtcggcttgt tcatcaccat gtaatggagc gattgcgggc tcccgacgac
    10501 ctgtcccgcc acttccacgc ggtcgcgggc cggatcgatc gctgtcccga gaagggtgac
    10561 gaccgtgcca ttgacccgga cctggcccgt ttcgaccagg cggtcggccc cccgccggga
    10621 ggcaacaccg gcccgggcca ggaatacgtt gatgcggatt gttgacggca ctatcccgtt
    10681 gccttcaaat gggttataaa caccgttgac ggcgggtcaa ggaaaaatgt actcgaaatg
    10741 caatccaacc taagtgtcat acaaaacaag gagttgtcag cttccgacgt ctttttcgac
    10801 atggggtgtt aaacgctccg acagtagcaa atccggaaac ctttgcgcgg gaaaggctta
    10861 gccgactctc cacattgaaa agaatttgtc cccgcggtgg gcgctggagt agaatcaaat
    10921 cactttcgct atggcatcct gtctgtcgcc gagggacagc cgcttgaatt cgatggcgga
    10981 cccggccgag tcactctaac aaaggggcac tggtacttga tgcagatcaa acaccaggga
    11041 cttggttcgc tcctcgtcga cgagggaatg ctcacccagg accaactggg caaggcgctc
    11101 caggagcaga cgagcacgca tgagcggctc ggggccatcc ttcttcgcct gggttacgtt
    11161 cgtgagccgg atcttgtgcg tgtccttgcc cgccagctcg gcgtttccac gttcgacccc
    11221 accatccaca aggtcatgcc cgacgcggtc agcctcgttc cgctcgacat ggcggagcga
    11281 tatgagctgg tgccggtcgt ccgcgagaac ggccgcctca cggtggcgat ggccgatccc
    11341 ctgaacctga tggcgctgga cgaccttcgc taccggaccc agaccgagcc catcgtcatg
    11401 atcggcctgg ccgacgacat cgcggttgcc cggcgcaagc actac
//
LOCUS       DASVOD010000132         2791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_374157, whole genome shotgun sequence.
ACCESSION   DASVOD010000132 DASVOD010000000
VERSION     DASVOD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374157"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2261
                     /locus_tag="VF720_02670"
     CDS             <1..2261
                     /locus_tag="VF720_02670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7878283.1"
                     /translation="VETGAGHAWAELIRRNGPLRVPFRDREEFLRHFWASSLPEDLEI
                     PDELKLSSQVAPPAPRLMVTKAPPERERDTLDAALWFDYDGGSVAEGQAARAVVNVDA
                     GRITTRDTAAEEAARALLIELGFREGPPPRSPDATLEIAPRHLGRVVRVLIGQGWQVE
                     AEGHRYRAPGIRRMSISSGIDWFDLTGAVDFGGQEASLPALLAAARKGLDFVLLDDGS
                     RGLLPEDWLERLSPLAELGVPEGEALRFHRSQATLLDALLASQPEVDKDADFRRLLER
                     LNAHSTIAPAEQPPGFVGKLREYQRLGLGWLQFLGEFAFGGCLADDMGLGKTIQVLAL
                     LEERRKKNTTRMPSLVVVPRSLVHNWIEEAARFTPELRVLDYTGQARAEHRPGFEAYD
                     LVVTTYGTVRRDVVELRSFRFDYVILDEAQAIKNATSQASRACRLLSSNHRLAMSGTP
                     VENHLGELGALFEFLNPGMLGRAAAARARVAGASDAESVAVLAKALKPFILRRTKEQV
                     LSELPTKTEQTLYCELEPKQRKMYDTMKAHYRQSLTATIGKMGLAKAKVYVLEALLRL
                     RQVACHPGLVDKSLVDESSAKIDTLFESLREVLDEGHKALVFSQFTSLLSIVRPRLEA
                     EGIKYEYLDGKTTKRSEKVKRFQEDPDCPVFLISLKAGGQGLNLTAADYVFILDPWWN
                     PAVEAQAVDRAHRIGQTRPVFAYRLIARDTVEEQIVELQRNKRHLADAIIAADSSLLK
                     SLTAEDLQRLLM"
     gene            2397..2588
                     /locus_tag="VF720_02675"
     CDS             2397..2588
                     /locus_tag="VF720_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878284.1"
                     /translation="MTMAIRHARTSTNPKTSAKKAAPATVRARKNKADQPPDGGNDER
                     AIARKNAKPRSSRQANRGA"
     gene            complement(2606..>2791)
                     /locus_tag="VF720_02680"
     CDS             complement(2606..>2791)
                     /locus_tag="VF720_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX7878285.1"
                     /translation="VLFDSGDEVVVQAGDEGVRFLLVSGEPLREPVAWYGPIVMNTQA
                     ELRKAFDDLDRGTFLRK"
BASE COUNT          487 a          972 c          903 g          429 t
ORIGIN      
        1 tcgtcgaaac gggcgcgggc catgcctggg ccgagctcat ccgccggaat gggccgctcc
       61 gcgtgccgtt ccgtgaccgc gaggaattcc tgcgtcactt ctgggcttcc tcgctccccg
      121 aggacctcga gatcccggac gaactgaagc tcagctcgca ggtggcgccg cccgcacctc
      181 gcctcatggt gacgaaggcg cctcccgaac gggaacgcga cacgctcgat gcggccctgt
      241 ggttcgatta cgacggcggc agcgtggccg aggggcaggc ggcccgcgcg gtcgtcaacg
      301 tcgacgcggg acggatcacg acacgcgaca cggcggccga ggaagcggcc cgggcgctcc
      361 tcatcgaact cggtttccgc gaagggccgc cgccacggtc gcccgacgcc acgctcgaaa
      421 tcgcgccgcg gcacctgggt cgcgtggtgc gcgtcctcat cggccagggg tggcaggtgg
      481 aggccgaggg gcatcgttac cgcgcgcccg gcatccgtcg catgtcgatc tcctcgggga
      541 tcgactggtt cgacctgacc ggcgccgtgg atttcggcgg ccaggaggcc tcgcttcccg
      601 cgctgctggc cgccgcgcga aagggtctgg acttcgtgct cctcgacgac ggttcgcgtg
      661 gcctcctgcc ggaagactgg ctcgagcgcc tctcgccctt ggccgagctc ggcgtgccgg
      721 aaggggaggc cctccgcttc catcgctcgc aggccacgct gctcgatgcg ctcctggcct
      781 cgcagcccga ggtggacaag gacgccgact tccggcgcct gctcgaacgc ctgaatgccc
      841 acagcaccat cgcaccggcc gaacagcccc ccggattcgt cggcaagctg cgcgaatacc
      901 agcgtctcgg cctcggctgg ctgcagttcc tcggcgagtt cgccttcggc ggctgcctgg
      961 ccgacgacat gggcctcggc aagacgatcc aggtgctcgc gctcctcgag gagcgccgca
     1021 agaagaacac gacccgcatg ccctcgctgg tggtggtgcc ccgcagcctc gtgcacaact
     1081 ggatcgagga agccgcgcgc ttcacgcccg aactgcgcgt gctcgactac accggccagg
     1141 cccgcgccga gcaccgtccc ggtttcgagg cgtacgacct cgtggtcacg acctacggca
     1201 ccgtccggcg cgatgtggtc gagcttcgct cgttccgatt cgattacgtc atcctcgacg
     1261 aagcgcaggc tatcaagaac gccacgtcac aggcctcgcg cgcctgtcgc ctgctcagca
     1321 gcaaccatcg cctcgccatg tcgggaacac cggtcgaaaa ccacctcggc gagctcggcg
     1381 cgctgttcga gttcctgaac cccggcatgc tcggccgtgc tgccgccgcc cgcgcgcgcg
     1441 tggccggcgc ctccgacgcc gaatccgtgg ccgtgctggc caaggcgctc aaacccttca
     1501 tcctgcgccg caccaaggag caggtgctct ccgagctccc gaccaagacc gaacagacgc
     1561 tgtactgcga gctcgagccc aagcagcgca agatgtacga caccatgaag gcccattacc
     1621 gccagagcct caccgccacc atcggcaaga tgggcctggc caaggctaag gtatacgtgc
     1681 tcgaggcatt gttgcggtta cgccaggtgg cctgccaccc cggcctcgtc gacaagtcac
     1741 tcgtggacga atcgagcgcc aagatcgaca cgcttttcga gagcctgcgc gaagtgctcg
     1801 acgaggggca caaggcgctc gtcttctcgc agttcacgag cctgctgtcg atcgttcgcc
     1861 cgcgcctcga ggccgaggga atcaagtacg aatacctcga cggcaagacg accaagcgct
     1921 ccgaaaaggt gaagcgcttc caggaagatc ccgattgccc ggtgttcctc atcagcctga
     1981 aggcgggcgg acaggggttg aacctcactg ccgccgacta cgtgttcatt ctcgatccgt
     2041 ggtggaaccc ggcggtcgag gcccaggccg tggatcgcgc gcaccgcatc gggcagacgc
     2101 gaccggtgtt tgcttatcga ctgatcgcgc gggacacggt cgaagagcag attgtggagt
     2161 tgcagcggaa caagcggcac ctggcggatg cgatcattgc ggcggattcg agtttgttga
     2221 agagtttgac ggcggaggat ctgcagcggt tgttgatgta gggcgcccgg gggattcccc
     2281 gatgttgttt cgccggcgtg cccgcacaat gtctaacgga ctattggata gagacccacg
     2341 acagaatgtc tccggcggca cgccggacca gataacctcg gccgcatcga gaggacatga
     2401 ccatggccat ccggcacgcg aggacatcga cgaacccgaa gacatcggcg aagaaggcgg
     2461 cgcctgccac cgtgcgcgcc cgcaagaaca aggcggatca gcccccggac ggcggtaacg
     2521 atgagcgcgc catcgcccgg aagaacgcca agccgcggag ttcgcgccag gcgaatcgag
     2581 gagcctgacg ggtcgggcgg gcctctcact tccggagaaa cgtcccccga tcgagatcgt
     2641 cgaacgcctt ccgcagttcc gcctgcgtat tcatcacgat cggcccgtac cacgccaccg
     2701 gctcgcgcag cggttcgccc gacacgagga ggaagcgcac gccctcgtcg ccggcctgga
     2761 cgaccacctc atcgcccgag tcgaacagga c
//
LOCUS       DASVOD010000133         4492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_843756, whole genome shotgun sequence.
ACCESSION   DASVOD010000133 DASVOD010000000
VERSION     DASVOD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843756"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VF720_02685"
     CDS             complement(<1..966)
                     /locus_tag="VF720_02685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7878286.1"
                     /translation="MTAGRKFLHILWQAPVYALPFAFFFALLFGGGEKAPFWSAVWAS
                     YKIAFTFSLSVQLLMFTVFWLSPLRMLREPPDGDRAAIIRTSIAYTLVATAGSILAAL
                     IIHQFVMPGFLGGGRQMALVGSFTLIFAVMGLAIAFAFQFHTTSMEKAKADQELTLAR
                     RIQESFLVREFPALAHLDVHAVNVSSQQVSGDFYDFVPAGNGRWLLAIADVTGKGVPA
                     ALLSSMLQASLRTQAGSEEQSPSRIVASMNRLAYRSTLTEQFATFFMACIDETTLSLS
                     YTNAGHNHPYVVRKDGRVEMLDQGGVIVGILEDFPYVEARVELAAG"
     gene            complement(966..2351)
                     /gene="mgtE"
                     /locus_tag="VF720_02690"
     CDS             complement(966..2351)
                     /gene="mgtE"
                     /locus_tag="VF720_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX7878287.1"
                     /translation="MTVRPEFESQASGLLFPEIREILHTAPSELPEVTEELHPADLAD
                     LVSKLEEDEVGIFFSNLPVDRAADVAEYVEETLRAHLVTALDPDRAARIVSAMSPDDR
                     ADVLADMEPEPAAAILARIALPQRRETQLLLQYEANTAGGIMTTQFVTLPLATRADHA
                     LALVKAAAQEKETIYTIFVIDRDQKLTGVVSLRELLVADAVQRLADIMNESVVWVTPE
                     TDQEEVARLISKYDILALPVVDEGRSMLGVVTVDDVIDVLVAEGTEDVQRMSAVSPIE
                     EPYLVAGFWHVMRNRVVWLVVLFLGELLTMNALQHYEDAFKAMSVLVLFIPLIISTGG
                     NSGSQTATLIIRALATGELKTGEYLRVLGRELMMGVALGVTLGGIGFIRAIVFPGADN
                     PDPVGVAAVVAFTIVAVVINGCVIGSLFPLLLKKLRLDPALMSAPFIASLIDVMGIVV
                     YFNIAQLLLRL"
     gene            complement(2390..>4492)
                     /locus_tag="VF720_02695"
     CDS             complement(2390..>4492)
                     /locus_tag="VF720_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878288.1"
                     /translation="LNPDGRDRYVSGQMQAVGRKPNTDRADREHQEAWPGGRYNHYLF
                     DLNRDWAWLSQPETKGRVAAFRAWHPQVHVDVHEMFFNSSYFFFPAAEPFHPTLPPQS
                     RAWGEVFGRGNAEAFDERGWRYFTAESFDLFYPGYGDSWPTLQGATGMTYEQGGQSGL
                     AIDRSDGTVLTLRERLRHHYVASMTTLAVARRERERRLLDYHAFFNDAVNGRSSAAAA
                     YLLPPVADEERRRELVELLLAHGIRVERATGEIKGANARGYDGQPMPGTLPAGTYVIP
                     GRQPLGYLAHALLEPETALPETLFYDITAWSLPMAFGLEAGYTDKLPGGSREMVTTTT
                     PEPGGVVDGPARTGWLLPWDRNAAPRLLNRLLNEGIRARFATKPFEIGGRYFQRGTIL
                     IPVNGNPDSTAALVDREARRAGVSVYAEKTGLTGTGIDLGSDRLLPVRTPRVAVVAGE
                     GIDPTSFGALWFLLDRQYEIDATVIPLEQLGSTNLSPYTALIFPDDDSGVGASYDDVL
                     DSLDVDRIERWVKAGGTFIGIKGGAGWATADHSGLTGLAIKHIDDEEEEHSDDKDDEA
                     ADEEKLRKKFLTTDERERERRLEAIPGAILRVELDPGHALAYGYEGEARVLKSGDLIF
                     EPTASGRNVAWYPPLSRVSGYISVDNEERLARTPFLAVESVGRGRAVLYNEDPNFRLF
                     WFGLNRLFLNSLFFAGGY"
BASE COUNT          733 a         1465 c         1577 g          717 t
ORIGIN      
        1 gccggcggcc agctccaccc gggcttcgac gtaggggaaa tcttccagga tcccgacgat
       61 cacgcccccc tggtcgagca tctccacgcg gccatccttc cggaccacgt aggggtggtt
      121 gtggccggcg ttggtatagg agagcgaaag ggtcgtttcg tcgatgcagg ccatgaagaa
      181 ggtggcgaac tgctcggtca gggtgctgcg gtaggccagc cggttcatgc tggccacgat
      241 ccgcgagggg gattgttcct ccgacccggc ctgggtccgg agcgacgcct ggagcatcga
      301 cgagaggagc gccgccggca cccccttgcc ggtcacgtcg gcgatggcca ggagccaccg
      361 gccgttcccg gccggcacga agtcgtagaa gtcgccgctc acctgctgtg acgagacgtt
      421 caccgcgtgc acatcgaggt gcgccagcgc cggaaattcc cgcaccagaa agctttcctg
      481 gatccggcgg gccagcgtga gctcctggtc ggccttggcc ttctccatcg acgtggtgtg
      541 gaactggaac gcgaaggcga tggccagccc catgaccgcg aagatcagcg tgaagctgcc
      601 gaccagggcc atctgccggc cgccacccag gaagccgggc atgacgaact ggtggatgat
      661 gagcgccgca aggatcgaac cggccgtggc caccagcgtg taggcgatcg aggtcctgat
      721 gatggcggcg cggtcgccgt ccggcggctc gcgcagcatc ctgagcgggg acagccagaa
      781 gaccgtgaac atcaggagct ggaccgacag ggagaaagtg aacgcgatct tgtaactggc
      841 ccacacggcg ctccagaagg gcgccttctc cccgccgccg aacagaaggg cgaagaagaa
      901 ggcgaagggg agcgcgtata ccggcgcctg ccagaggatg tgcaggaact tgcggccggc
      961 ggtcatcaga gccggagaag cagctgggcg atgttgaagt agacgacgat ccccatcacg
     1021 tcgatcaggc tggcgatgaa gggcgccgac atgagggccg ggtcgagccg cagcttcttg
     1081 agcaggagcg ggaagagcga gccgatcacg cagccgttga tgacgacggc cacgatggtg
     1141 aacgccacga cggcggccac gccgaccgga tcgggattgt ccgcccctgg gaagacgatg
     1201 gcccggatga agccgatccc gccgagcgtg acgccgagcg ccacgcccat catcagttca
     1261 cggccgagca cgcgcaggta ttcgccggtc ttgagttcgc cggtggccag ggcccggatg
     1321 atcagcgtgg ccgtctggct tcccgaattt ccgccggtcg agatgatcag cgggatgaag
     1381 agcaccagta ccgacatcgc cttgaaggcg tcttcgtaat gctgcagggc gttcatggtg
     1441 aggagctcgc cgagaaagag caccaccagc cacaccacgc ggttgcgcat gacgtgccag
     1501 aagccggcca ccaggtacgg ctcctcgatc ggcgagacgg cgctcatcct ctgcacgtcc
     1561 tcggtacctt cggccacgag gacgtcgatg acgtcgtcga cggtcaccac gcccagcatc
     1621 gagcgccctt cgtccaccac cggcagcgcc aggatgtcgt acttcgagat gaggcgtgcc
     1681 acttcctcct ggtccgtctc gggcgtcacc cacacgaccg actcgttcat gatgtcggcc
     1741 agccgctgca cggcgtcggc caccagcagc tcccggagcg acacgacgcc ggtgagcttc
     1801 tggtcacgat cgatgacgaa gatcgtgtag atcgtctcct tctcctgggc ggccgccttc
     1861 accagcgcga gcgcgtggtc ggcgcgcgtg gccagcggca gcgtgacgaa ctgcgtcgtc
     1921 atgatgccgc cggcggtgtt ggcctcgtac tgcagcagca gttgcgtctc gcgccgctgc
     1981 ggcagggcga tgcgggccag gatggcggcg gccggctccg gctccatgtc ggccagcacg
     2041 tcggcgcgat cgtccgggct catggccgaa acgatgcgcg cggcccggtc gggatcgagg
     2101 gccgtcacca ggtgcgcgcg gagcgtctcc tccacgtact cggccacgtc ggccgcccgg
     2161 tcgaccggga ggttcgagaa gaagatgccg acttcgtctt cctcgagctt gctgaccagg
     2221 tcggccaggt cggccgggtg cagctcttcg gtgacttcgg gcagctccga cggcgcggtg
     2281 tgcaggatct cccggatttc cgggaagagg aggcccgagg cctgcgactc gaactcgggg
     2341 cgcaccgtca tgatgactcc gtccgcacgc gtgtcgccgg catgacgctc tagtacccgc
     2401 cggcgaagaa gaggctgttg aggaagaggc ggttcaggcc gaaccagaag aggcggaagt
     2461 tggggtcctc gttgtagagc acggcgcggc ctcggccgac cgactccacc gccaggaagg
     2521 gggtgcgggc cagccgttct tcgttgtcca cggaaatgta gcccgacacg cgcgacagcg
     2581 gcggatacca ggccacgttc cgaccggaag cggtcggttc gaagatcagg tcgcccgact
     2641 tgaggacgcg ggcctctcct tcatagccgt aggccagggc gtggccggga tcgagctcca
     2701 cgcgcaggat ggcaccggga atggcctcga ggcgccgctc ccgttcgcgc tcgtcggtgg
     2761 tcaggaactt cttgcggagc ttttcctcat cggcggcctc gtcgtccttg tcgtcgctgt
     2821 gctcttcctc ctcgtcatcg atgtgcttga tggccaggcc ggtcaggccg gaatgatcgg
     2881 cggtggccca accggcgcct cccttgatcc cgatgaacgt gccgccggct ttcacccagc
     2941 gttcgatccg gtcgacgtcg aggctgtcga ggacgtcgtc gtagctcgcg ccgactccgg
     3001 agtcatcgtc cgggaagatg agcgccgtat agggcgagag gttggtcgat cccagctgct
     3061 cgagcggaat gaccgtggcg tcgatctcgt actgtcggtc caggaggaac cagagcgcgc
     3121 cgaacgacgt ggggtcgatt ccctcgccgg ccaccaccgc cacgcgaggc gtgcgcaccg
     3181 gcagcagtcg gtcggagccc aggtcgatgc cggtgccggt aaggcccgtc ttctcggcgt
     3241 acacgctcac gcccgcccgg cgggcttcgc gatccacgag ggccgcggtc gagtcgggat
     3301 tgccgttcac cggaatcagg atggtgccgc gctggaagta gcgacctccg atctcgaacg
     3361 gcttggtggc gaagcgggcc cggattccct cgttgagcaa ccggttcagg agcctcggcg
     3421 ccgcgttccg gtcccacggg agaagccagc cggtgcgggc cgggccatcc accacgcccc
     3481 cgggttccgg ggtggtggtg gtgaccatct cgcgactgcc gccggggagc ttgtcggtgt
     3541 aaccggcctc gaggccgaag gccatcggca gcgaccaggc ggtgatgtca tagaagagcg
     3601 tctccggaag cgccgtttcc ggctcgagga gcgcgtgggc caggtagccg agcggctgcc
     3661 gccccgggat gacgtaggtg ccggccggga gcgtgcccgg catcggctgg ccgtcgtagc
     3721 cgcgggcgtt ggctcccttg atctcgccgg tggcgcgttc cacgcggatg ccgtgggcca
     3781 ggaggagctc caccagttcg cgccggcgtt cctcgtcggc caccggcggg agcaggtacg
     3841 ccgccgcggc gctcgagcgt ccattgacgg cgtcattgaa gaaggcgtgg tagtcgagca
     3901 gccgccgctc gcgctccctc cgcgccacgg ccagcgtggt catcgacgcc acgtagtggt
     3961 ggcgcaggcg ctcgcgcagg gtgaggacgg tgccgtcgct ccgatcgatg gcgagtcccg
     4021 actggccccc ctgttcgtag gtcatcccgg tggcgccctg cagggtgggc cacgagtcgc
     4081 cgtatcccgg gtagaagagg tcgaacgact cggcggtgaa gtatcgccac ccccgttcgt
     4141 cgaaggcctc ggcattgccg cgcccgaaca cctcgcccca ggcccgcgac tgcggcggga
     4201 gcgtgggatg gaacggctcg gcggccggga agaagaagta gctcgagttg aagaacatct
     4261 cgtggacgtc cacgtgcacc tgtggatgcc aggcgcggaa ggcggccacg cgccccttcg
     4321 tttccggctg actgagccag gcccaatcgc gattcaggtc gaagagatag tgattgtagc
     4381 gtccccccgg ccaggcttcc tggtgttcgc ggtcggcccg gtcggtattc ggcttccgtc
     4441 ccaccgcctg catctgcccg ctcacgtaac gatcgcgccc atccgggttg ag
//
LOCUS       DASVOD010000134         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_321973, whole genome shotgun sequence.
ACCESSION   DASVOD010000134 DASVOD010000000
VERSION     DASVOD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321973"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /gene="carB"
                     /locus_tag="VF720_02700"
     CDS             <1..891
                     /gene="carB"
                     /locus_tag="VF720_02700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX7878289.1"
                     /translation="HIEEAGIHSGDSSCVIPPWSIGEETTRRLSEITRAVALDLNVVG
                     LINVQFAVHNDAIYVIEVNPRGSRTIPFASKAVGRPLARIATRVMVGKKLADMGLTTP
                     PAPTHVSVKKAVLPFERFPGEDTLLGPEMKSTGEVMGIGEDFGRAYAKAQLGSGEGLP
                     LTGTVFLSIRDADKRSIVFLSKKLLELGFRIVATGGTARFLKMNGQIVESVNKIIEPA
                     PNVMDWIVSGRIHMVINTPEGGPSQHDDQAIRMAAVARGIPVITTIPGAMAAVSGIES
                     IRSSGMTVAPIQALAPVLEA"
     gene            895..>2603
                     /locus_tag="VF720_02705"
     CDS             895..>2603
                     /locus_tag="VF720_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878290.1"
                     /translation="MNPPTGAGRRLPRWSLAVFLALALSSTGCAYYNTFYLAKRYYRD
                     AVTEEERNTTDRISPAATAAYEKAIKQCARILQKYRGSKWVDDALLLTGKSFYGQGNY
                     GEARRWLSQLVNEHPKSELLPEAQVWIGRTWLAEEDYEKSGSVLHGVLADIPQFEARS
                     EAVFFAAETELRQEHWEQAIKEFRELIERYPKSERVGQALFLVGDAQFGLRQYQEAER
                     SYRQSAARAREPRDRMQALLKVGQMLEKQERFDEATHLYDGLSAEIVPRDKLNTILSG
                     FEAALPNQPLQGNAANSGLNEQQLIEQGSYQDANGNTVFPNPNFPRDNVTPNGNPNNL
                     SADPAAPAAAPPDPRRTGEGNAAARGALNLAIASNPLAADLPQVLLRQGNALMEMGEY
                     DKAIKTFESVLASTPHTSEAGEAQYRIGYILEVYLEDYAGARIAYDLVKQHGAGLFVE
                     QATRRSGGLERLVALAAQDSTRATGANASLEAEAEKQFLAAELAWFQQEKPEKAIDLY
                     AEVERNYPNTSFAPRSALARAWLLANVMADTTAGREAFESVTVTYADTEQARIAHQIL
                     TGEV"
BASE COUNT          484 a          909 c          834 g          376 t
ORIGIN      
        1 cacatcgagg aggccggcat ccactccggc gattcctcct gcgtcatccc gccatggtcc
       61 atcggcgagg aaacgacgcg gcgactgtcc gagatcaccc gggcggtggc tctcgacctg
      121 aacgtggtgg ggctcatcaa cgtgcaattc gcggtccaca acgacgccat ctacgtcatc
      181 gaggtcaatc cgcgcggctc gcgcacgatt cccttcgcca gcaaggcggt gggacgaccg
      241 ctcgcgcgca tcgccacccg cgtgatggtc ggcaagaagc tggccgacat ggggctcacc
      301 acgccgccgg cacccacgca cgtgagcgtg aagaaggcgg tcctgccgtt cgagcgcttc
      361 ccgggcgagg acacactgct cggccccgag atgaagtcca ccggcgaggt catgggaatc
      421 ggcgaggact tcggccgcgc gtacgccaag gcccagctcg gctcgggcga gggactgccg
      481 ctgaccggca cggtgttcct ctcgatccgc gacgccgaca agcgatcgat cgtcttcctc
      541 tcgaagaagc tcctcgagct cggtttccgg atcgtggcca ccggcggcac cgcccgcttc
      601 ctcaagatga acggccagat cgtggagagc gtgaacaaga tcatcgagcc ggcacccaac
      661 gtcatggact ggatcgtgtc gggacgcatc cacatggtga tcaacacgcc ggaaggtggc
      721 ccgagccagc acgacgacca ggccatccgc atggcggcgg tggcccgcgg catccccgtc
      781 atcaccacca tcccgggagc gatggcggcc gtgagcggca tcgagtcgat ccggagttcc
      841 ggcatgacgg tcgcgccgat ccaggcgctg gcacccgtcc tcgaggcgta ggggatgaac
      901 ccgccgaccg gcgccgggcg ccggttgcca cgctggagcc tcgcggtgtt cctggcactt
      961 gccctctcat cgaccggctg cgcgtactac aacaccttct atctcgccaa gcggtactac
     1021 cgggacgccg tgaccgagga ggaacggaac accaccgacc gcatttcccc cgcggccacc
     1081 gcggcgtacg agaaagccat caagcagtgc gcccgaatct tgcagaagta ccgcggttcc
     1141 aagtgggtcg atgacgcact tctgctcacc ggcaagagct tctacggcca ggggaactac
     1201 ggcgaggcgc gtcgctggct cagccagctc gtcaatgagc acccgaaaag cgagctcctc
     1261 cccgaggcgc aggtctggat cggtcggacc tggctggcgg aggaggatta cgagaagtcg
     1321 ggaagcgtcc tgcacggcgt cctggccgac atcccgcagt tcgaagcgcg ctccgaggcc
     1381 gttttctttg ccgcggaaac cgagttgcgc caggagcact gggagcaggc catcaaggaa
     1441 ttccgcgagc tcatcgagcg ctaccccaag agcgagcggg tgggccaggc cctcttcctg
     1501 gtgggcgatg cgcagttcgg cctgcgccag taccaggagg ccgagcgctc ctatcgccag
     1561 tcggcggccc gcgcccggga gccgcgcgat cgcatgcagg cgctgctcaa ggttgggcag
     1621 atgctggaga agcaggagcg gttcgatgaa gccacgcatc tctacgacgg cctctcggcc
     1681 gagatcgttc cgcgcgacaa gctgaacacc atcctgtcgg ggttcgaggc cgcgctgccg
     1741 aaccagcccc tgcaaggcaa tgcggccaac agcggcctca acgagcagca gctgatcgag
     1801 cagggctcct accaggacgc caacggcaac acggtattcc ccaatcccaa cttcccgcgc
     1861 gacaacgtca cgccgaacgg caatccgaac aacctctccg ccgatcctgc cgctccggcg
     1921 gccgcgccgc ccgatccacg ccggaccggc gaagggaacg ccgcggcccg gggcgccctc
     1981 aacctggcca tcgcgagcaa ccccttggcc gccgacctcc cccaggtgct tctccggcag
     2041 ggaaacgcgc tgatggagat gggggagtac gacaaggcca tcaagacttt cgagtcggtg
     2101 ctggcgtcca cgccgcacac ctccgaagcg ggggaggccc agtatcggat cggctacatc
     2161 ctcgaagttt atctcgagga ttacgcgggc gcgcgcatcg cctatgacct cgtgaagcag
     2221 catggagccg gcctcttcgt ggagcaggcc acccggcgct cggggggact ggagcggctg
     2281 gtggcgctcg ccgcccagga ttccactcgc gccacgggcg ccaacgcctc gctcgaagcc
     2341 gaggccgaga agcagttcct cgccgccgag ctggcgtggt tccagcagga aaagcccgag
     2401 aaggccatcg atctctacgc cgaagtcgaa aggaattatc cgaacacctc attcgcgccc
     2461 cggtcggcac tggcccgggc ctggctcctc gccaacgtca tggccgatac gaccgccggc
     2521 cgcgaggcct ttgagtcggt cacggtcacg tacgccgata ccgaacaggc gcgcatcgcc
     2581 caccagatcc tgaccggcga agt
//
LOCUS       DASVOD010000135         6061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_217629, whole genome shotgun sequence.
ACCESSION   DASVOD010000135 DASVOD010000000
VERSION     DASVOD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6061
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217629"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1925)
                     /locus_tag="VF720_02710"
     CDS             complement(30..1925)
                     /locus_tag="VF720_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878291.1"
                     /translation="MATILWLALILLLPGSSAAQVTSSTNPPATTATPPVEASEGNPP
                     QASTLEPLVSLNGQGVMTHVVEAQTAYRGGLESGRRGQGEAARRLFAEAARLDPTFPD
                     PWFATAASWLPFRPDMAMASVIEGCKSSWRSFRGQHRLIMNSALLLLGVLVFSLMGTT
                     FLIALRGLRHFQHPIFEVLRRRLPVMAAAIAGWIIVLQPLLWGLGAFLSLMLASGLLW
                     FYVGKGERRVLAAYATLAIIVPMAFHGLSRLSAPLNPESVPYLLSAASETPDQPGLSD
                     ALARAMQDNPADPGPHMALGLTMEHAGQYEGAEKEYRAALENQGDEGRVRNNLGNIMA
                     LSGRLDGAVEQYQKSIATAPWLAAPHFNLSQIYARRLQFDLADNEMKQASQLDFESVR
                     SHTASLGQKKAGLVSLGLAPRDLWSATLARPAAFPIGVPRSMAWLYGGAMGMLPLFSI
                     ALIALGLLVGQALHRFLPTYACANCDCVVCRKCLRRIRRRAYCEPCGDTILSMQTSEF
                     TRMLLERRLNQDHWGRRLARLALVLAIPGWEAVRRGRPMIGLTVMTGFVLLIVPVITG
                     GRAIGPTPGLAGFSPGPLWSVLLPGLFVLYGLSALLLKLMPEPESALIGQDLAGPSGR
                     DRLDRAA"
     gene            2121..3422
                     /gene="asnS"
                     /locus_tag="VF720_02715"
     CDS             2121..3422
                     /gene="asnS"
                     /locus_tag="VF720_02715"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX7878292.1"
                     /translation="MDVSAVSYIEDLGRHVGAAVTVKGWIYNSRHSGKLAFLLVRDGT
                     GTVQGVVSKKDVDEATWLASESATQEAAVTMTGVVRADERAPGGYELTVTEVRGVSPA
                     VDYPITPKEHGTDFLMDHRHLWLRSSRQHAILRVRHELVAGIRDFLNGRGFTLIDAPI
                     FTPNACEGTSNLFETDYFDDKAYLTQSGQLYMEAGAAAFGRVYCFGPTFRAEKSKTRR
                     HLTEFWMVEPEVAWADLDDNMRLIEEFVSALVGRLLDKRKEELKRLERDTTLLEKAVP
                     PFPRVHYDEAAATLKAAGVDFQYGGDFGGTDETVLTEKQEKPIFVHRFPAAVKAFYMK
                     GDPERPDLSLSCDLLAPEGYGEIVGGGQREESLDVLLGKIHEHGLPESAFQWYLDLRR
                     YGSVPHSGFGLGVERTLAWVCGLPHVRETIPFPRMLSRLSP"
     gene            3548..4372
                     /locus_tag="VF720_02720"
     CDS             3548..4372
                     /locus_tag="VF720_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7878293.1"
                     /translation="MAQWRSFRTLLVVAIVALMATLAGDLARAQEGTSTGSASTEFKT
                     DLPSQVPDEPAPPPVRPDGSFLTVAAQSGGTIFWVLVVMAIGTVLVALERFLALQKGR
                     VDVSRLMSDIVKALNDKGPNAAMEIASRNKGPVGQIVMYGLQRVARGSEGVEKAVETA
                     ALAQVVGMRRGLGILNFVGQSAPLLGFLGTIMGMLHAFDQAVGTGRVNAPQAIGGISE
                     AVITTTTGIAVAIVANIFLNLITARIDQVTVQMEEAGDELVDAVHAGGARLKGRTA"
     gene            4650..6035
                     /locus_tag="VF720_02725"
     CDS             4650..6035
                     /locus_tag="VF720_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7878294.1"
                     /translation="MISRRPAGMAVFAIMLSALLIPGVAACADDETRPEVALDRPLSA
                     DEAVRLALDRNYRIAQATDLKGIQDANKLNAWGGFVPSLNLGYDFSRSGSKGSFQGDN
                     LEISIPERTGQSDGFSLGASQTLFSLPVIFNIISAGKNSGAAGEEVRNAEQTAAQIVR
                     TQYYNVIEAIKLEEVAREDVRLAGEELKRTQSLFDVGSVARTDVLKSNVRVSEAQSAL
                     TSAVNRIDVERALLNQALALPPTTVVQLIESLEPRADNPDSVQAYAQAVENRPDLVAA
                     RLRLEGARADQKAAIGGKIPYISHSFRASDITSDGDVQVTGEDNVPRFVSGNINSDSW
                     SYQIGATWNILDGLVTESQIQRAKHNKHLQENQYHDLENTILVETTQALVALRNANAQ
                     IIVAREARVSAEEDLKLSQERYSVGLGTILELIDAQVNVSRARSGEVTAMAALKKAEA
                     QLDKAVGRTNW"
BASE COUNT         1100 a         1966 c         2036 g          959 t
ORIGIN      
        1 aggttgcccg agagtcccat gaatggttcc taggccgcgc gatccagacg gtcccgaccc
       61 gatggaccgg ccaggtcctg gccgatcagc gccgattccg gttccggcat cagcttgagc
      121 aggagggcgg agaggccgta cagcacgaag aggccgggga gcagcaccga ccagagcggc
      181 cccggtgaga agccggccag tccgggggtg ggcccgatgg ccctgccacc ggtgatcacc
      241 gggacgatca ggaggacgaa accggtcatc acggtgaggc cgatcatcgg gcgcccgcgg
      301 cggacggcct cccagccggg aatcgccagc accagggcga gccgggccag ccggcggccc
      361 cagtgatcct ggttgagccg gcgctcgaga agcatgcggg tgaactcgga cgtctgcatg
      421 gaaaggatcg tgtcgccgca tggctcgcag taggcgcggc gccggatccg tcggaggcac
      481 ttgcgacaca ccacgcaatc acagttcgca caggcgtagg tcggcaggaa ccgatggagg
      541 gcctgcccga cgaggagccc gagtgcgatc agcgcgatgc tgaagagggg gagcatcccc
      601 atggcgccgc cgtacagcca ggccattgag cgtggaacgc cgatggggaa tgccgccggg
      661 cgggccagcg tagccgacca gagatccctg ggtgcgaggc cgagcgaaac gagaccggcc
      721 ttcttctgtc cgagcgacgc ggtatgtgac cggacgctct cgaaatcgag ctgcgaggcc
      781 tgcttcatct cgttgtcggc cagatcgaac tggagacggc gcgcgtagat ctggctcagg
      841 ttgaagtgcg gcgccgccag ccagggggcg gtggcgatcg acttctggta ctgctcgacg
      901 gcaccgtcca ggcggccgct cagggccatg atgttgccga ggttgttccg cacacgtccc
      961 tcgtccccct ggttctccag cgcggcgcga tattcctttt cggctccctc gtactgtccc
     1021 gcatgttcca tcgtgaggcc cagggccatg tgcggcccgg gatcggcggg gttgtcctgc
     1081 atggccctgg ccagagcgtc ggaaagaccg ggctggtcgg gggtttcgct ggcggccgac
     1141 aggaggtagg gaacggattc cgggttcagc ggggccgaca ggcgtgacag gccatgaaag
     1201 gccatcggca cgatgatggc cagcgtggca tacgcggcca ggacccggcg ctcccccttg
     1261 ccaacgtaga accagaggag gcccgacgcc agcatgagcg agaggaaagc gccgaggccc
     1321 cacagcagcg gctgcaggac gatgatccat ccggcgatcg cggcagccat cacgggaaga
     1381 cgacgacgaa gcacttcgaa aatggggtgc tggaaatggc gaaggccacg aagcgcgatc
     1441 aggaacgtcg ttcccatcag gctgaagacg agaacgccga gcaggagcag cgccgagttc
     1501 atgatcaggc ggtgctggcc ccggaaggat cgccacgatg acttgcaccc ctcgatcacg
     1561 ctggccatcg ccatgtcggg acggaagggg agccaggagg ccgcggtggc aaaccaggga
     1621 tcggggaagg tgggatcgag ccgggccgct tcggcgaaga gacggcgggc cgcctcaccc
     1681 tgcccgcgcc ggcccgattc caggccgccc cgataggccg tctgcgcttc caccacatgc
     1741 gtcatgacac cctggccgtt caggctcacc agtggctcga gggtggacgc ctggggcgga
     1801 ttgccctcgg aagcctccac cgggggagtt gcggtcgtgg cgggagggtt ggtcgacgag
     1861 gtcacctgcg cggcgctgga accggggaga agcaggatga gggcgagcca tagaatggtt
     1921 gccactgcct tcgcgccgcc tctcaagcga cgagggtaac cacaagaaat cattctagtt
     1981 aacctccatg gaggtgaaat tgacagcgca tctggaggcc gctatagtgc cggctctttg
     2041 ttgaaacggg atgggccgat tccaccattc agtgcggtta tcggcgcata ggttcattct
     2101 caaaggaaaa agttgggagg gtggacgtgt ccgccgtcag ctatatcgag gacctggggc
     2161 ggcatgtggg tgccgccgtc accgtgaagg ggtggatcta caacagccgg cacagcggga
     2221 agcttgcctt cctcctcgtg cgggacggca ccggcaccgt gcagggtgtt gtcagcaaga
     2281 aggatgtcga cgaggccacc tggctggcca gcgagtcggc cacccaggaa gcagccgtca
     2341 cgatgaccgg cgtggtgcgc gccgatgaac gcgcccccgg gggctatgaa ctcaccgtca
     2401 ccgaggtgcg gggcgtctcc ccggccgtcg actacccgat cacccccaag gaacatggca
     2461 ccgacttcct gatggaccat cgccacctct ggctccggtc cagccgccag cacgccatcc
     2521 tccgggtgcg gcatgaactg gtggccggca tccgcgactt cctcaatggc cgcgggttca
     2581 cgctgatcga cgcgcccatc ttcacgccca acgcctgcga agggacgagc aacctcttcg
     2641 agaccgacta cttcgacgac aaggcctacc tcacgcagag cggccagctc tacatggaag
     2701 cgggtgccgc ggccttcggc cgcgtctact gtttcgggcc cacgttccgc gccgagaaat
     2761 cgaagacgcg ccggcatctc accgagttct ggatggtcga gccggaagtg gcatgggccg
     2821 acctcgacga caacatgcgt ctcatcgagg agttcgtctc cgcgctggtg gggcggctcc
     2881 tcgacaagcg caaggaagaa ttgaagcgtc tggagcgcga caccacgctc ctcgaaaagg
     2941 cggtgccgcc atttccgcgg gttcactacg acgaggcggc ggccactctc aaggcggccg
     3001 gtgtcgactt ccagtacggc ggtgatttcg gcggcacgga cgaaaccgtg ctcaccgaga
     3061 agcaggagaa gccgatcttc gtccaccgtt ttccggcggc ggtcaaggcg ttctacatga
     3121 agggagaccc cgagcggccg gatctctccc tgtcgtgcga cctgctggcc cccgaggggt
     3181 acggcgaaat cgtcgggggg gggcagcgcg aggagtcgct cgacgtgctg ctcggcaaga
     3241 ttcacgagca cggcctgccg gagtcggcct tccagtggta cctcgacctc aggcgttacg
     3301 ggagcgtccc ccattcgggg ttcgggctcg gcgtcgagcg aaccctggcc tgggtgtgcg
     3361 gcctgccgca cgtccgggag acgatcccgt tcccacgcat gctgtcgcgg ctctccccct
     3421 aggaggtgac gttgacagcc ggcggggcgg tatttaccat gccccggcgg tcgtaaccac
     3481 ccctcggacc gcctgttccg caatcacaac cgactgactc cgtaggaccg taaaggagat
     3541 tccgttcatg gctcagtgga ggtctttccg aacgctgctc gtggtcgcca tcgtggccct
     3601 gatggcgact cttgccggcg atctggcccg cgcccaggag gggacgtcga cgggtagcgc
     3661 gagcacggag ttcaagaccg acctgccgag ccaggtgccc gacgagccgg caccccctcc
     3721 ggtccgcccg gacggttcct tcctcaccgt ggccgcccag tccggcggca cgatcttctg
     3781 ggtgctcgtg gtcatggcca tcggcaccgt cctcgtggcg ctcgaacgat tcctggccct
     3841 gcagaagggg cgggtcgatg tctcccgcct gatgagcgac atcgtgaagg ccctgaacga
     3901 caaagggccg aacgccgcca tggaaatcgc ctcccgcaac aagggccccg tcgggcagat
     3961 cgtcatgtac ggcctgcagc gcgtggcccg cggctccgaa ggcgtggaaa aggccgtcga
     4021 gacggccgcg ctggcccagg tcgtcggcat gcgccgcggc ctcggcatcc tgaatttcgt
     4081 cggccagtcg gcgccgctgc tcggcttcct cggcaccatc atggggatgc tccacgcctt
     4141 cgaccaggcc gtaggcaccg ggcgcgtcaa cgccccgcag gccatcggcg gcatttccga
     4201 ggccgtcatc accacgacca ccggcatcgc cgttgcgatc gtggccaaca tcttcctgaa
     4261 cctgatcacg gcccggatcg accaggtcac ggtccagatg gaagaggcgg gcgatgagct
     4321 cgtcgacgcc gtccatgccg ggggagcccg tctcaaggga cgaactgcct gatccagaga
     4381 aaagcccgca acgaccggcg cgggtttccc gtccaagaag cggcggtggc gccatgccgg
     4441 attccttccg gatagcgccg acaatcgaat ccagatcccg ccggtcccat tcgatcggtg
     4501 ggattttttt tgcaacccgt cctgcatgat tccgtaaccc ggtgtgagtt cgcgggaaac
     4561 ggccgccccg cgcggtcccg cccgcatcct cgccccgatc cgacgacagt ccgcggtgcc
     4621 gggctgcctt gcctcatcaa gggggaaaca tgatctccag acgaccggcc ggcatggccg
     4681 tgttcgccat tatgctgagt gccttgctta ttcccggcgt tgccgcttgt gcagacgacg
     4741 aaacccgtcc ggaggtcgcc ctcgatcgac ccctgtcagc cgatgaggct gtccgcctgg
     4801 cgctggaccg gaactaccgg atcgcccagg cgaccgacct caagggaatc caggacgcga
     4861 acaagttgaa tgcctggggc ggattcgtgc cgtcgctgaa tctcgggtac gacttctccc
     4921 gcagcgggtc caaggggagt ttccaggggg acaacctgga gatctccatc cccgaacgta
     4981 cggggcagtc cgacgggttt tcgctgggcg cgtcgcagac tctcttctca ctcccggtca
     5041 tcttcaacat catctctgcc ggcaagaatt ccggcgcggc cggtgaggaa gtgcgcaacg
     5101 cggagcagac cgcggcacag atcgtccgga cccagtacta caacgtcatc gaggcgatca
     5161 agctggagga ggtggcgcgc gaagatgtgc gccttgccgg cgaggagctc aagcggaccc
     5221 agagcctgtt cgacgtcggc tcggtggccc gcaccgacgt gctgaagtcc aatgtccgcg
     5281 tctcggaggc gcagtcggcg ctgacctctg ccgtcaaccg gattgatgtc gagcgggctc
     5341 tgctcaatca ggccctggcc ctcccgccga cgaccgtggt ccagctcatc gaaagcctgg
     5401 agccgcgtgc cgacaatcct gactccgtcc aggcgtatgc ccaggccgtg gagaaccggc
     5461 ctgatctcgt ggcggcgcgg ctgcgactgg aaggagcccg ggccgaccag aaggccgcaa
     5521 tcggcgggaa gattccgtac atcagccaca gcttccgggc cagcgacatc acttcggacg
     5581 gcgacgtgca ggtaacgggt gaggacaacg tgccccgctt cgtgtccggc aacatcaatt
     5641 cggattcctg gtcctatcag atcggcgcaa cctggaacat cctcgacggg ctggtcaccg
     5701 aaagccagat ccagcgggcc aagcacaaca agcatctgca ggaaaatcag tatcacgacc
     5761 tggagaacac catccttgtg gagaccaccc aggccctcgt ggcgcttcgc aacgcgaatg
     5821 cccagatcat cgtggcgcgc gaagcccggg tttccgccga agaggacttg aagctttcgc
     5881 aggagcgtta ctcggtgggc ctggggacga ttctcgagct catcgatgcg caggtgaacg
     5941 tctcgcgcgc ccgttcgggc gaggttaccg ccatggccgc gctcaagaag gccgaggcgc
     6001 agctcgacaa ggcggtcggt cgcaccaact ggtagacgcg cggggagttc ccggcgcgcg
     6061 g
//
LOCUS       DASVOD010000136         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_583013, whole genome shotgun sequence.
ACCESSION   DASVOD010000136 DASVOD010000000
VERSION     DASVOD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583013"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF720_02730"
     CDS             <1..660
                     /locus_tag="VF720_02730"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX7878295.1"
                     /translation="WYRRLSLLDHVLRDDATFEEFDRADFGEQGDFLVQPWDVRVAEP
                     TGTGTPQGAELTLRRAGTAWLGGRARQLVFEKRIRMTADAPRIEIEHVIRSMESTPLE
                     FRHGIEFNFTMLAGDADDRWVAIDGTKPAGQPNLGGRGVHAGVRTVSLHDAWEPFSVV
                     LNWTRPADLWRLPVDTISQSEAGFERVYQSTCLLPQWRVALEPNGEWRQAITLTFHDR
                     A"
     gene            703..1467
                     /locus_tag="VF720_02735"
     CDS             703..1467
                     /locus_tag="VF720_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEX7878296.1"
                     /translation="MNWLVILGLLFLAAWAAADTAAFGQFMVGQPLASGWLAGVIIGR
                     PIEGLFLGGALQMLWSRLAPVGAAAYPDVGPATVGGIGSLHFWTASIRGAGEPATWSE
                     RFGFVSSPWEWRSALDPMALMLGLGIALATGAVGQRLVVAMRRHNADLGRAADAAAER
                     GDFGGIERANLLGLTRAAGLGVVVTAGALLVGAVGATLASRVPHAPAGSPNPGVLLFW
                     WIALAALGTALWSGGRRDWLWLLAGVAGGAIFLVTR"
     gene            1472..2275
                     /locus_tag="VF720_02740"
     CDS             1472..2275
                     /locus_tag="VF720_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEX7878297.1"
                     /translation="MNLARHRLDEQELPLPRRLAIVARSFFLQSAWSFERMQSVGFAT
                     LMSGEGKRLTRGRPDAAAEFLKRHLTFFNTNPPMAGYLAGVSVRLEEAAAAASDPAES
                     ARLLDDLHRFKRAGVSAFAAWGDTFFWATLRPAATAVALLVALFAGAWGALAWLIVYN
                     AAHLHYRYHGVREGYRYGPAVAGRVGGSRLKRLPDMTRRVGLLSVGALVPLSVVGARV
                     DFGTVGLAAFPAVGVAMVAILRRRGGLGWEWGLLAVILGIAWSVATAGR"
BASE COUNT          317 a          828 c          867 g          424 t
ORIGIN      
        1 tggtatcgac gtctctcgct gctcgatcac gtgttgcgcg atgatgccac gttcgaagag
       61 ttcgatcggg ccgatttcgg cgagcaggga gatttcctcg tccagccgtg ggacgtgcgc
      121 gttgccgagc cgacgggcac cggcacaccc cagggcgccg agctcacgct gcggcgcgcg
      181 ggcacggcct ggctcggcgg gcgggctcgg caactggtct tcgaaaagcg gatccggatg
      241 acggccgacg cgccgcgtat cgagatcgag catgtcatcc ggtcgatgga gtccacgccg
      301 ctcgagttcc ggcacggcat cgaattcaac ttcacgatgc tggcgggcga tgcggacgac
      361 cggtgggtgg cgatcgacgg cacgaagccg gccggacaac ccaaccttgg agggcgcggc
      421 gtgcacgccg gcgtgcgcac ggtgagcctg cacgatgcct gggagccgtt ctccgtggtg
      481 ctcaactgga cgcggccggc cgatctctgg cgactgccgg tcgacaccat ctcccagtcg
      541 gaagctggat tcgagcgcgt ctaccagagt acctgccttc ttccacagtg gcgcgtggct
      601 ctcgagccga atggcgagtg gcggcaggcg atcacgctca cgtttcacga ccgggcctga
      661 ggcgggtcga ggcgggatgc gggacgggat gacgttgtgg atgtgaactg gctcgtcatc
      721 ctcggactgc tgttcctggc ggcctgggcc gccgcggaca cggctgcctt cggccagttc
      781 atggtgggac agccgcttgc ctccggctgg ctggcggggg tgatcatcgg ccggccgatc
      841 gagggactct tcctgggagg cgcccttcag atgctctgga gccgcctcgc cccggtcggc
      901 gccgcggcct accccgacgt gggtccggcc acggttggcg gcatcggctc gctccacttc
      961 tggaccgctt cgatccgcgg cgcgggggag ccggcaacct ggtccgagcg attcggcttc
     1021 gtttcctccc cgtgggaatg gcgttcggca ctcgatccga tggcgctcat gctcgggctg
     1081 ggcatcgcgc tcgcgaccgg ggccgtcggg cagcggctcg tggtggccat gcgccggcac
     1141 aacgccgatc tcggtcgtgc cgcggacgcg gcggccgagc ggggtgattt cggcggcatc
     1201 gaacgcgcga acctcctggg tctcacacgg gccgccgggc tcggcgtggt ggtaaccgcc
     1261 ggtgcgcttc tggtcggagc ggtcggggct accctcgctt cgcgtgtgcc gcacgcaccc
     1321 gccggcagcc cgaacccggg ggtgctcctc ttctggtgga tcgccctggc ggcactcggc
     1381 acggccctct ggagcggcgg ccgccgcgac tggctgtggc ttctggccgg cgtggctggc
     1441 ggcgccatct tcctcgtgac ccggtgattc catgaacctg gcccgccatc gcctcgacga
     1501 gcaggaactc ccgttgccgc gccgtctcgc gatcgtcgcg cggtcgttct tcctccagtc
     1561 ggcatggagc ttcgagcgga tgcaatcggt cggcttcgcc acgctcatgt caggcgaagg
     1621 gaagcggctc actcgaggtc gtccggatgc cgcggccgaa ttcctcaaac gccacctcac
     1681 cttcttcaac accaatccgc ccatggccgg ctacctggcg ggcgtatcgg tacgactcga
     1741 ggaggcggcg gccgccgctt ccgacccggc cgagtcggcg cgcctcctcg acgacctcca
     1801 ccgattcaag cgggccggcg tgagcgcttt cgccgcctgg ggcgacacct tcttctgggc
     1861 caccctgcga ccggccgcca cggcggtcgc cctcctggtg gccctcttcg ccggtgcgtg
     1921 gggggcgctg gcgtggctga tcgtctacaa tgccgcgcac cttcattacc gataccatgg
     1981 cgtccgcgaa gggtaccggt acggcccggc cgtggccggt cgggtcgggg gatcacgcct
     2041 gaaacgcctg ccggacatga cccggcgggt cgggctcctc tccgtgggcg ctctcgtccc
     2101 tctgtccgtg gtgggcgccc gggtcgattt cgggacggtg gggctcgcgg cctttccggc
     2161 cgtcggggtg gccatggtgg ccattctcag gcgtcgtggc gggctcggat gggagtgggg
     2221 cctcctggcg gtcatcctcg ggatcgcgtg gagcgtggcc accgccggcc gataactggg
     2281 aaaaactttc gagatcgaga cccgatgcct gaaacgaccg tcaccatcac caacgaaaaa
     2341 ggaatgcacg cccgggccgc cgcgctcttc tcccgggagg ccagcctcca caaggccagc
     2401 gttttcgtgg cccgcgaggg tctggaagtg aatggc
//
LOCUS       DASVOD010000137         2527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_374264, whole genome shotgun sequence.
ACCESSION   DASVOD010000137 DASVOD010000000
VERSION     DASVOD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374264"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1041)
                     /gene="pstC"
                     /locus_tag="VF720_02745"
     CDS             complement(94..1041)
                     /gene="pstC"
                     /locus_tag="VF720_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX7878298.1"
                     /translation="MAKGGIRRGGVPRSRWFTVRERLIEGTIAVCSAVAILGVFLILI
                     SVVREAIPIFFTAEDPAAANLHGFFGQPVWQPVSDHPRYSLLPLVLGTAKITIIAMAV
                     AIPIGVLAALYSAEFAPRWVGEIVKPFVELLAGIPSVVMGFFALIVLASLVQAITGAT
                     ARLNAVNAGLALGLAIIPVVYSVSEDALRAVPNTLREASLALGCNNWKTAVHAVLPAA
                     SSGIFASIVLGLGRAVGETMVVLMASGNAALISWSPLDTTRTMSATIAAEMGEVVVGG
                     DHYRVLFLIGVMLFGFTLVLNIVAGWYLSAMRRRLTGEA"
     gene            complement(1085..>2527)
                     /locus_tag="VF720_02750"
     CDS             complement(1085..>2527)
                     /locus_tag="VF720_02750"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7878299.1"
                     /translation="RSIRLHYLALVVATVTLSGLAMLVMPAAAALLVGLMTGLGGAWW
                     LAAGIAAPLEDLALRARGWAEGTIEQLPPRHGGGDEVDAVTAVLANLGTLLEAREGSQ
                     EHGARQLRQILETMVEGVMVLAPDGRVTLENAAMRRLVGTTGPLAGRTAIEALRISEF
                     EAAARDIQAGGPAADLELVLDAVVSLDPREAAPMGAASRRTFRVSLAPLAVEQGRSVV
                     AVFHDISQVRELENLRRDFVANVSHELRTPLTAIKGYVETLRDGEIAPADRERFLDIV
                     ARHSNRLSVLIEDLLELSRLESPETRLDIRPNSLPAAAQRALALLDGAAHLAEVTLVS
                     GMPADLPAVVADARSLEQILINLLDNAIKHTGKGGRVMLSASRQEGRVLVSVADTGIG
                     IPAHALPRVFERFYRVDQGRSREHGGTGLGLAIVKHLVALHGGEVGVESEPGKGSRFW
                     FTLRVAGSEPAVAIVTESSPGADRSVTESA"
BASE COUNT          445 a          881 c          849 g          352 t
ORIGIN      
        1 acagccccgt gaggaacacc tcggcccagg cgatgcggcg cccgccgatg aggggaagcc
       61 cgcggcgacg gatcgagggg gacgtggcgg ccatcaggcc tccccggtca ggcggcgacg
      121 catggcggag agataccagc cggccacgat gttcagcacg agcgtgaagc cgaagagcat
      181 cacgccgatc aggaaaagga cgcggtagtg atcgccgccg acgaccacct cgcccatctc
      241 ggccgcaatg gtggccgaca tcgtgcgcgt tgtgtcgagc ggactccagg agatgagcgc
      301 ggcgttcccc gaggccatca gcaccaccat cgtctcgccg acggcgcggc cgagtccgag
      361 gacgatcgag gcgaagatgc cggacgaggc ggccgggagc acggcgtgga ccgcggtctt
      421 ccagttgttg caccccagcg ccagcgacgc ctcacgcagg gtgttcggaa cggcccgtag
      481 cgcgtcctcg ctgaccgagt agacgaccgg gataatggca aggccgagcg ccaggccggc
      541 gttcacggcg ttcaggcggg ccgtcgcccc ggtgatcgcc tgcaccagcg aggccagcac
      601 gatcagggcg aaaaagccca tgaccacgga gggaatgccg gccagcagct cgacgaacgg
      661 cttcacgatt tccccgaccc agcgcggggc gaactcggcg ctgtacaacg ccgcaaggac
      721 gccgatcggg atggccacgg ccatggcgat gatcgtgatc ttggccgtgc cgagcacgag
      781 cggaagaagg gagtagcggg gatgatcgga caccggctgc cagaccggct gtccgaagaa
      841 accatggagg ttggcggcgg caggatcctc ggccgtgaag aagatcggga tggcctcccg
      901 gaccacggaa atgaggatga ggaagacccc gaggatcgcc accgcggagc agaccgcgat
      961 cgtcccctcg atcagccgtt cccggacagt gaaccaacgg ctgcgcggca caccgccccg
     1021 tcggattccg cctttcgcca tgtgaccggt tcctccttgc ctcgtcggta atcccgaatg
     1081 aaggctaggc ggactccgtg acggaacggt ccgcgcccgg tgacgattcg gtgacgattg
     1141 ccaccgcggg ctccgacccc gccacccgca acgtgaacca gaaccggctc cccttgccgg
     1201 gctcgctttc cacgccgacc tcccccccgt ggagggccac caggtgcttc acgatggcca
     1261 gcccgaggcc cgtgccaccg tgctcccggc tgcgcccctg gtccacccgg tagaaacgct
     1321 cgaagacgcg cggaagggcg tgcgccggta tcccgatgcc ggtgtcggcc acgctcacca
     1381 ggacgcgtcc ctcctgccgg gaggccgaaa gcatgacgcg cccccccttc ccggtgtgct
     1441 tgatcgcatt gtccagcagg ttgatcaaga tctgctccag actcctggcg tcagccacga
     1501 ctgccggaag atcggccggc atcccgctga ccagcgtgac ctccgccagg tgcgccgcac
     1561 catcgagcag ggccagcgcg cgctgtgcgg ccgctgggag ggagttcgga cggatgtcga
     1621 ggcgggtctc gggtgattcg aggcgggaga gttccagcag gtcctcgatc agtacggaca
     1681 gccgattgga gtgccgcgcc acgatatcca ggaaccgctc ccggtcggcc ggcgcgattt
     1741 ccccgtcccg gagcgtttcc acgtacccct tgatggcggt cagcggcgtc ctcagttcgt
     1801 gagacacgtt ggccacgaag tcgcgacgca ggttttcgag ttcccgtacc tgggaaatgt
     1861 cgtggaaaac cgccacgacc gatcgcccct gctcgactgc caggggcgcc agcgacaccc
     1921 ggaaggtgcg tcgtgatgcg gctcccatcg gagcggcctc gcgcggatcg agcgacacaa
     1981 ccgcatcgag cacgagttcg agatcggcgg cgggtccgcc cgcctggatg tcgcgcgcgg
     2041 ccgcctcgaa ttcggaaatg cgcagggcct cgatggccgt tcttccggcc agcgggccgg
     2101 tggtcccgac caaccgccgc atcgccgcgt tttccagcgt gacgcgaccg tccggggcca
     2161 ggaccatgac cccttccacc atcgtctcga ggatctggcg gagttgccgg gcgccatgtt
     2221 cctgcgatcc ttcccgcgct tcgaggaggg tgcccaggtt tgcgagaacc gccgtgaccg
     2281 catcgacttc atccccgccg ccatgacgcg gcggcagttg ctcgatcgtc ccctccgccc
     2341 agccacgggc gcggagcgcc agatcctcga ggggagcggc aatgccggcc gccagccacc
     2401 aggcgcctcc cagacccgtc atcagcccca cgaggagggc cgccgcggcc ggcatcacga
     2461 gcatcgccag gcccgaaagc gtcaccgtcg ccacgactag ggccaggtaa tgaagtcgaa
     2521 tggatcg
//
LOCUS       DASVOD010000138         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_166359, whole genome shotgun sequence.
ACCESSION   DASVOD010000138 DASVOD010000000
VERSION     DASVOD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166359"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VF720_02755"
     CDS             complement(<1..1011)
                     /locus_tag="VF720_02755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7878300.1"
                     /translation="MWLPLLSITLLFAAPSPSGRWIAERRLVNDAWTGRIVATDAGTT
                     FDLGPHVFPIGWQGKDDLLRVVRLVDDGHELIEAASGFVHPADGAVVTWKEDESVAGV
                     ADAWRRAYAAVAPPPVTASAAPLVVAIDPGHGGPGAGTWNGNNGDGAGARGPNGLTEQ
                     WVNLRVAGFLAADLARDPRFAGSFLLRSSATAAVTLADRVALAAQGGAGFYLSIHHNG
                     LPTGAANRTEAYWCGLNAHAGECPDMAWRLHDRIVDAFGYTGRGAFEDSAGTGRFHFY
                     VLRHAAMPAVLTEASNLNDAAEEDRFGHDPVERHAKDEAVALHLGLIDLAFGTTGVTE
                     GAP"
     gene            complement(1090..2418)
                     /locus_tag="VF720_02760"
     CDS             complement(1090..2418)
                     /locus_tag="VF720_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7878301.1"
                     /translation="MESAPLEERPKGGRSFRPRLKRPLPTPTLLRGYLVVGSLVLASF
                     ALFYANRVVNRLNDQTRALSAVMARVIALSSAQVREYPDSSLVELYKELIGPITFPII
                     ITDPEGRPRVRGNSPQLRAIRLTDEELSAEDLRRPGPELALLLDRVDQFDRKQPPEPL
                     ISPRDGSVLGYLHYGPTGIVEEIRWVPWLLFGVAALFATVGIVWFRSLRRSEQNRIWA
                     GMAKETAHQLGTPISSLMGWLELARDQAVARPDGVTELPTALFADVEREIAADLERLR
                     KVASRFGHIGSLPALSPQDIVPIVASTVGYYRRRLPQQGLGMTLTESFDPDVPPVNVN
                     AELMGWVVENLVKNAIDASDAKTGHIDVSVRRRPESETVEISVKDNGRGIPAAQQRRV
                     FEPGFSTKKRGWGLGLTLVRRIVEDYHGGRVELRSSAPGQGSEFVVCFPV"
     gene            complement(2429..3700)
                     /gene="serS"
                     /locus_tag="VF720_02765"
     CDS             complement(2429..3700)
                     /gene="serS"
                     /locus_tag="VF720_02765"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX7878302.1"
                     /translation="MLDARYLRTNPEGISDAIAAKNGDPAHFARFLAADADYRKVLAE
                     VEQLRAERTKGSEEVGRRRKAGEDAAALQAEVKAMGDRLKAAEERLGPLEAAAEEALH
                     WIPNLAHPSVPVGDATHNTVVKSWKEPRTFDFPVLPHDELAPKLGLLEMARGTAMSGP
                     GFSVLKGAGARLERALINFFLDVHTGEHGYTEVAAPFLVKREALFGTGQLPKLEDDMY
                     RTTDDLFLIPTAEVSITNLHRDEILDGAQLPLHYVGFSPCFRREAGSYGKETKGLTRV
                     HQFMKVEMVRITTPEKSYAEHEQLLADAEDILRRLDLPYRVLLLASGDTSFAAAKCYD
                     LEVYAPGADRWLEVSSCSNFEAFQARRLKMRYKAEAGAKPEFVHTLNASGLALPRIVI
                     AILENYQTKDGRVRVPDVLVPYLGGLRELGG"
     gene            complement(3764..4756)
                     /gene="hemB"
                     /locus_tag="VF720_02770"
     CDS             complement(3764..4756)
                     /gene="hemB"
                     /locus_tag="VF720_02770"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX7878303.1"
                     /translation="MPFPEERPRRLRGSVRRRAMVAETRLHRDRLIWPLFAVPGTNVE
                     REIPGFAGVQHHSVDRLVEACRRAVGDYGLRSVLLFGLPEAKDAEGSGAWAEEGIVQQ
                     ACRAIRAAKLDIEVITDVCLCEYTDHGHCGVLTPELPNRPATVDNDPTLELLARTAVS
                     HAAAGADMVAPSDMMDGRIGALRAALDDEGHDDLPIMAYSAKYASAFYGPFRLAADSA
                     PAFGDRRTYQMDPANADEAMREIALDIDEGADIVMVKPGLPYLDIIRRAKDTFPVPIA
                     AYNVSGEYVMLKALAAGDAAAEERLFLEATTSLCRAGADLVITYAAPLVARAIR"
     gene            complement(4760..>5190)
                     /locus_tag="VF720_02775"
     CDS             complement(4760..>5190)
                     /locus_tag="VF720_02775"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX7878304.1"
                     /translation="AEGLVELLADTPGVLSGRRVVILRAVEGGVTLPNGLTRAGAAAR
                     LIFAYRTAIMEGAARELAERLQKWPADDPDAIIVTSGMPLIALTGRLSKEHRTRVPIV
                     AIGPVTAEMAGTLGFRVAAVAEQPTPAALASAIERYFSPR"
BASE COUNT          818 a         1768 c         1794 g          810 t
ORIGIN      
        1 gggcgctccc tccgtcacgc ccgtcgtgcc gaaggccagg tcgatgagtc ccaagtgcag
       61 cgccacggcc tcgtcctttg cgtgccgctc caccggatcg tgtccgaatc ggtcctcctc
      121 ggcggcatcg ttcaggttgc tggcctcggt caggaccgcc ggcatcgccg cgtggcggag
      181 cacgtagaag tgaaagcgcc cggtgccggc ggaatcttcg aaggcgccgc gacccgtgta
      241 gccgaacgca tcgacaatcc ggtcgtggag ccgccatgcc atgtccgggc attcgccggc
      301 gtgggcgttg agaccgcacc aataagcctc ggtgcggttg gcggcgccgg tcggcaggcc
      361 attgtggtgg atcgagagat agaaaccggc cccgccctgc gccgccagcg ccacgcggtc
      421 agcgagggtg acggcggcgg tggcgctgct cctcaggagg aacgacccgg caaagcgggg
      481 atcgcgcgcg aggtcggccg cgaggaaacc cgccacgcgc aggttcaccc actgctcggt
      541 aagcccgttc ggcccgcggg cgcccgcgcc gtcgccgttg ttgccgttcc aggtgccggc
      601 gcccggaccg ccatgccccg gatcgatggc cacgacgagc ggggccgcgc tcgccgtgac
      661 gggtggcgga gccacggcgg cataggcccg gcgccaggcg tcggcaaccc ccgcgaccga
      721 ctcatcctcc ttccatgtga cgaccgcgcc gtccgccgga tggacgaacc ccgacgcggc
      781 ctcgatcaac tcatggccgt catccacgag gcggacgacg cgcagcaggt cgtccttccc
      841 ctgccaaccg atcggaaaga cgtgcgggcc aaggtcgaac gtcgtgccgg cgtcggtggc
      901 gacgatccgt ccggtccagg cgtcgttgac gagacgccgc tcggcgatcc atcgaccgga
      961 tggggacggc gcggcgaaga ggagcgtgat ggaaagcagg ggaagccaca tgtggcggta
     1021 cccggcgggt tggagaggga tgtgggcggc cgcaaggcta atcgcgatgc ggttcgagcc
     1081 gtcaagtggt tacacgggaa aacagacgac gaactcgctc ccctgccccg gtgcgctcga
     1141 gcggagctcg acgcggccgc cgtgatagtc ctcgacgatg cggcgcacca gcgtgaggcc
     1201 gagcccccag ccacgcttct tggtgctgaa ccccggctcg aagacgcgcc gctgctgggc
     1261 cgccgggatc ccccgtccat tgtccttcac cgagatctcg acggtttcgg attcgggccg
     1321 gcgccgcacg ctcacgtcga tgtggccggt cttcgcatcg gaggcatcga tcgcgttctt
     1381 gaccaggttc tccaccaccc atcccatcag ctcggcattg acgttgacag gggggacatc
     1441 gggatcgaac gattcggtca gcgtcattcc cagcccctgt tgcggcaggc gccgacgata
     1501 gtagccgacg gtcgaggcca cgatcggcac gatgtcctgc ggcgagagcg ccggcagcga
     1561 accgatgtgg ccgaatcggc tggccacctt gcgcagtcgt tccaggtcgg cggcgatttc
     1621 ccgctccacg tccgcgaaga gggcggtggg gagttcggtc accccgtcgg ggcgcgccac
     1681 cgcctggtcg cgggcgagct ccagccatcc catcagggag gagatgggcg taccgagctg
     1741 gtgggccgtc tccttggcca tgccggccca gatccggttc tgctcgctgc ggcggaggct
     1801 gcggaaccag acgatgccga ccgtggcgaa gagggccgcc acgccgaaga ggagccacgg
     1861 aacccagcgt atctcctcca cgatgccggt cgggccgtag tggaggtaac cgaggacgct
     1921 cccgtcgcgc gggctgatca acggctccgg cggctgttta cggtcgaact ggtcgacgcg
     1981 gtcgagcagc agcgccagct ccggaccggg gcggcgcagg tcttcggccg agagctcctc
     2041 gtccgtcagg cggatggcgc gcaactgcgg gctgttcccc ctcacgcggg gacgtccttc
     2101 cggatcggtg atgatgatcg ggaaggtgat cgggccgatc agttccttgt agagttcgac
     2161 cagcgagctg tcgggatact cgcgcacctg ggcgctggac agcgcaatga cgcgggccat
     2221 caccgccgag agcgcgcgcg tctggtcgtt gagccggttg acgacccggt tggcgtagaa
     2281 gagggcgaac gaggcaagga cgaggctgcc gacgaccagg tagccgcgga ggagggtcgg
     2341 cgtcgggagc gggcgtttca ggcgcggccg gaacgaccgc ccacctttcg gcctctcctc
     2401 gaggggtgcc gattccatca ggaaacgatc aacctcccag ttcccggagc ccgccgaggt
     2461 acggcacgag gacgtcgggt acccgcaccc gcccatcctt cgtctggtag ttctcgagga
     2521 tggcgatcac gatccggggg agcgccaggc ccgacgcgtt gagcgtgtgc acgaactcgg
     2581 gctttgcgcc ggcctcggcc ttgtagcgca tcttgaggcg ccgggcctgg aaggcctcga
     2641 agttgctgca gctcgacacc tccagccagc gatcggcgcc cggcgcgtac acctcgaggt
     2701 cgtagcactt ggccgccgcg aatgacgtat cgccgctggc gagcaacagc acccggtacg
     2761 gcaggtcgag gcgccggagg atgtcctcgg cgtcggccag gagctgttcg tgctcggcat
     2821 acgacttctc gggagtcgtg atgcggacca tctccacctt catgaactgg tggacgcggg
     2881 tcagcccctt cgtttccttg ccgtacgaac cggcctcgcg gcggaagcag gggctgaacc
     2941 ccacgtagtg gagaggcagt tgcgccccgt cgagaatctc gtcgcgatgc aggttggtga
     3001 tcgacacctc ggccgtcggg atcaggaaga ggtcgtcggt cgtccgatac atgtcgtctt
     3061 cgagcttcgg gagctggccg gtgccgaaga gagcctcgcg cttcacgagg aagggcgccg
     3121 ccacctcggt gtaaccgtgc tctccggtgt gcacatcaag gaagaagttg atcagggccc
     3181 gctcgaggcg cgcgccggcc cccttgagca ccgaaaaccc ggggccgctc atcgccgtgc
     3241 cgcgcgccat ctcgaggagg ccgagcttcg gagccagctc gtcgtgcggc agcaccggaa
     3301 agtcgaacgt gcgcggctcc ttccacgact tcacgaccgt gttgtgcgtg gcatcgccca
     3361 ccggaacgga gggatgggcc aggttcggaa tccagtggag cgcttcctcg gcggcggctt
     3421 cgagcgggcc gagcctttcc tcagcggcct tgagccgatc gcccatggcc ttcacctcgg
     3481 cctgcagcgc ggccgcgtcc tcgcccgcct tgcggcgccg ccccacctct tccgagccct
     3541 tcgtccgttc ggcacggagc tgttccacct cggccagcac cttccggtag tcggcatcgg
     3601 ccgcgaggaa acgggcgaaa tgcgccggat cgccgttctt ggcggcgatg gcgtcggaaa
     3661 tgccttcggg attggttcgc aggtatcggg cgtcgagcac gcagctactc ctctacgacg
     3721 gatttgtcag ggacgggtgc gatcgaatcc ggcagtctat cagtcaacga atcgcccgcg
     3781 cgacgagcgg cgccgcgtag gtgatgacga gatccgcgcc cgcccggcag agcgacgtgg
     3841 tggcctcgag gaagagccgc tcctcggctg cggcatcacc cgccgccagc gccttcagca
     3901 tcacgtattc gccgctcacg ttataggcgg cgatcggcac cgggaacgtg tccttcgccc
     3961 tgcggatgat gtcgaggtac ggaagccccg gcttcaccat cacgatgtcg gccccttcgt
     4021 cgatgtcgag ggcgatctcg cgcatcgcct cgtcggcgtt ggccggatcc atctggtagg
     4081 tgcgccggtc gccgaaggcg ggcgccgaat cggccgccag gcggaacggg ccgtagaaag
     4141 ccgaggcgta cttggccgag taggccatga tcggaagatc gtcgtgccct tcgtcgtcga
     4201 gcgccgcgcg cagggcgccg atccggccgt ccatcatgtc gctcggcgcc accatgtcgg
     4261 cgccggccgc cgcgtgcgac acggccgtgc gcgccaggag ctccagcgtc gggtcgttgt
     4321 cgaccgtggc cggtcggttc ggcaactcgg gcgtgaggac gccacaatgc ccgtgatcgg
     4381 tgtactcgca gaggcagaca tcggtgatga cttcgatgtc gagcttcgcg gcccggatcg
     4441 cacggcacgc ctgctgaacg attccctcct cggcccaggc acccgagccc tccgcgtctt
     4501 tcgcctcggg caggccgaac aggagcaccg agcgcagacc gtaatcgccc accgcccgcc
     4561 gacaggcctc gacgagccgg tccaccgaat gatgttgcac gcccgcgaag cccgggatct
     4621 cccgctcgac gttcgtgccg ggcacggcaa acaggggcca gatgagccgg tcgcgatgca
     4681 ggcgcgtctc ggccaccatg gcccggcgac gtacggagcc gcgcaggcgg cggggacgtt
     4741 cctcgggaaa gggcatcgat tacctcggtg aaaagtaacg ttcgatcgcg gacgccaggg
     4801 cggccggtgt cggctgctcc gccacggccg ccacccggaa gccgagcgtg ccggccatct
     4861 cggccgtgac cggtccaatg gcaacaatcg gtacgcgtgt tcgatgttcc ttcgacagcc
     4921 gccccgtcaa cgcaatcagc ggcattccgc tggtcacgat gatcgcgtcc ggatcatcgg
     4981 caggccattt ctgcaatcgc tccgccagct ccctggccgc gccctccatg atagcggttc
     5041 ggtacgcgaa gatgagccgg gcggcggcgc cggccctggt cagcccgttc ggcaaggtga
     5101 caccaccctc gaccgcgcgc aggatcacga cgcggcggcc cgatagcacc cccggtgtgt
     5161 ccgccaggag ttccaccaat ccctcggccc
//
LOCUS       DASVOD010000139         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_687158, whole genome shotgun sequence.
ACCESSION   DASVOD010000139 DASVOD010000000
VERSION     DASVOD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687158"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1550)
                     /locus_tag="VF720_02780"
     CDS             complement(<1..1550)
                     /locus_tag="VF720_02780"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878305.1"
                     /translation="MNFPPVPFSLLLAPALVATLLSPALAVERAMVLHGGDRTLASVV
                     APAGPVTLNLASVGLFPSEMVRFGQHAWVPCGGSDRVDVVDLAGDLNPDPPAIVTGPG
                     TNPYGVAFAGASKIYVSLLTSNAVAVANPLSGGVAATIPVGRSPQGVLFAAGRIFVAN
                     TGFDFSTFGYDPGTVSVIDPATDAVIATLPVGTNPQALALAPNGEVHVICTGNYFSEF
                     GRVFVIDPNVPAVVADFAVGGSPGFIAISPAGTGYVSDYFLGLLSYDVATRTLLHGDG
                     DPIAVGTGAAGVDFDTAGRVYAAVYGDDQVVVLGADDSILNVHPLGDGPQDVVLYDSD
                     EPVAVALADFAAALDGGDVTLSWRTQGETDHAAFQVERSAVSVDGPWGEAGRVTSGGA
                     DHGRYSFVDRAPFDGSTERLWYRLVAIDRDGGAQAFAPAVVERPATGAIAGLALGAPA
                     PNPLRSGVTRLALHLAFESRVVLTVHDPAGREVARPLESLLSAGDHAVTWDGHAADGR
                     ELARGVYFL"
     regulatory      complement(1701..1885)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(1933..3453)
                     /locus_tag="VF720_02785"
     CDS             complement(1933..3453)
                     /locus_tag="VF720_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878306.1"
                     /translation="MSKCLWSALRAGILIGFFVIGCSAPTGQATPPASPAPPTARAAL
                     VEYRRIGDAGAGADSADLALNWRPVNPGHAARGGWSSSVWLASLPDGETKRRYILDCT
                     GCHQLNETRVFRGDSLRTAVQWSTDAARMIAFAGSESGFPVISHDADAESLGAFLSSH
                     LRAAPPANAAATYEALVAPPPRDSDALRARITEYQLPVAPDLPHDVAVTADGSVLVTG
                     MFTSRIYRVNPDSGVVGVENIPVEKANPRAIELDAAGNWWVLCGAPMKVARYEPAGAR
                     WTSWDIGMYPHSIAPSSDGSRIWFNGHFTRDPEQIGWLDPATGQVTTATAPRHPELGA
                     QPGGPIPYEARLAPNGTLWVSELQGNRLLGHHPGTGAWKTWSMPTSWSGPRRFDVGPD
                     GVLWIPAYANGTLVRFDPSTGAVEELPLPIADSVPYVVRVDPRNGQVWIGTAAADVLY
                     RYSPAERRFDILPIPTRGASMRHLAVDPARGAVWVAYGASPAIHPARVARIQILPD"
     gene            complement(3456..3833)
                     /locus_tag="VF720_02790"
     CDS             complement(3456..3833)
                     /locus_tag="VF720_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7878307.1"
                     /translation="MKIHLDTSFLIRAMIRDTVQDLRLREWLTAGESVAITAIAWTEF
                     LCGPVGPTDVELARELIREQVAYHEEDASLAARLYNQSGRRSRSLTDCMIAASCIRSE
                     ATLATTDAKDFQRLIPLGLRLLT"
     gene            complement(3830..4108)
                     /locus_tag="VF720_02795"
     CDS             complement(3830..4108)
                     /locus_tag="VF720_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX7878308.1"
                     /translation="MATPSVKTTYSLDVKTLRALDGMAKRWKVSRSEALRRIILTAAS
                     APAPPEEDPIAILNRLQKSLALTEVEADEWVERVRDERRAFPRKTVRK"
     gene            complement(4158..4805)
                     /locus_tag="VF720_02800"
     CDS             complement(4158..4805)
                     /locus_tag="VF720_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7878309.1"
                     /translation="MRATFAFVTLAVLALVVLAGPTHAQSDSTAAPTTKAWQVWMGGG
                     ISFPASGTFKDTYDSGWNARGMLSYRSAGMPIGLRLDVGAQEFDFTSSGIDASIRALS
                     VLLDITHDFQLGTIQPYVAVGAGGHSIEPGPETVESVRSRPRAPSREVEDNLEYDPVE
                     PGWRFGLNGVIGLKLPISPRMMIFAEGRIDQVFVEKAILGQDDLQMIPVTFGIVF"
     gene            4737..>4918
                     /locus_tag="VF720_02805"
     CDS             4737..>4918
                     /locus_tag="VF720_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878310.1"
                     /translation="MGGAGQNDESEYRQGDESKRRSQFGLVNRREWPEKSPPDGVHRP
                     IGLVELLRIVDFVPFPL"
BASE COUNT          850 a         1667 c         1652 g          749 t
ORIGIN      
        1 aggaagtaaa ccccgcgggc gagctcacgg ccatccgccg cgtgcccgtc ccacgtgacc
       61 gcgtggtcgc cggccgacag gagcgactcg agcggccggg ccacttcgcg gccggcggga
      121 tcgtgaacgg tcaagaccac ccggctctcg aaagccaggt gcagcgcgag ccgcgtcacg
      181 ccggaacgaa ggggattcgg ggccggcgcc cccagggcca accccgcgat cgcgcccgtc
      241 gccggccgct cgaccaccgc gggcgcaaac gcctgcgcgc caccatcgcg atcgatggcc
      301 accagccgat accagagccg ttcggtcgag ccgtcgaacg gggcccggtc gacgaacgag
      361 taccgaccgt gatcggcccc gccggacgtc acgcgcccgg cctcgcccca cggcccgtcg
      421 accgaaaccg ccgaacgctc aacctgaaag gccgcatgat ccgtctcgcc ctgcgtccgc
      481 cacgagagcg tcacgtctcc accgtccagc gccgccgcga aatcggccaa cgccaccgcc
      541 accggttcgt ccgagtcgta cagcaccacg tcctgcggcc cgtcacccag cggatgcacg
      601 ttcaggatcg agtcgtcggc gccgagcacc acgacctggt cgtcgccata caccgccgcg
      661 tacacccggc cggcggtgtc gaagtcgacc ccggccgcgc cggtgccgac cgcgatggga
      721 tcgccgtcac cgtgcaggag cgttcgcgtg gctacgtcgt acgacaggag cccgaggaaa
      781 tagtcgctca cgtagccggt gcccgcgggg ctgatggcga tgaaccccgg tgatcctccc
      841 accgcgaagt cggccacgac ggccggcacg ttcggatcga tcacgaagac ccgaccgaat
      901 tccgagaagt agttgccggt gcagatcacg tgcacttcac cgttcggcgc gagggccagc
      961 gcctgcggat tggtgccgac cgggagcgtg gcgatgaccg cgtccgtggc cgggtcgatc
     1021 acgctcaccg tgccggggtc atagccgaac gtcgagaagt cgaaaccggt gttggccacg
     1081 aagatcctgc cggcggcgaa cagcacgccc tgcggcgaac ggccgaccgg gatggtggcg
     1141 gccactccgc cggacaacgg attggcgacg gccacggcat tggaagtaag gagcgagaca
     1201 tagatcttgc tcgccccggc gaacgccacg ccatacggat tcgtgccggg accggtcacg
     1261 atcgccggcg gatcggggtt caggtctccc gccaggtcaa cgacatccac tcggtcgctg
     1321 ccgccgcacg gcacccaggc atgctggccg aatcgcacca tctcgctcgg aaaaagtccg
     1381 accgaggcca ggttcaacgt gacgggaccg gcgggggcca cgacggaggc aagcgtgcga
     1441 tcgcccccgt ggaggaccat ggcccgctcg acggccaggg cgggggagag gagcgtcgcg
     1501 acaagtgcgg gcgcgagcaa cagcgaaaag ggtacgggag gaaaattcat ggacgagcct
     1561 ccttcgggtc cgaaaccgtc actcgcggtc gggcacacac gaaggagaaa gcggagcgcg
     1621 ccccggcagg agccgggcag gcgacagcgg gcgggttgcc gagcgggaaa ccgtccgtcc
     1681 accttcccct cgaaggtgtg ctgttggacc gtttccccgg ccggtctcct ggctcgcgat
     1741 tcgtcctact acccgcccct tcccggaagc ccgaaggcca ccagtgggat ttcgcggggt
     1801 tcgtcctcgc tcacagtggc ggggccgcgt cggtcttgca ccgacttccc gttcaccggg
     1861 gaacaccgac gacgggccgt cgatcggtcc gccatcgtcg atcggaacgc tacggcaggc
     1921 gaggcgtccg cgtcaatccg gaagaatctg gatgcgggcc acgcgcgccg ggtggatggc
     1981 cggcgaggca ccatacgcca cccacaccgc gccacgggcc ggatcgaccg ccaggtggcg
     2041 catcgaggcc ccgcgcgtcg ggatggggag gatgtcgaag cgccgctccg ccggcgaata
     2101 gcgatacagc acgtcggccg ccgcggtgcc gatccacacc tggccattcc gcggatcgac
     2161 gcgcacgaca tatggcaccg agtcggcgat cggcaacggc agttcctcga ccgccccggt
     2221 ggacggatcg aaccggacca gcgtgccgtt cgcgtaggcc ggtatccaca acacgccgtc
     2281 cggccccacg tcgaatcggc gcggcccgct ccacgacgtc ggcatcgacc acgttttcca
     2341 ggctcccgtg ccggggtgat ggccgagcag ccggtttccc tgcagttccg atacccacaa
     2401 cgtgccgttg ggcgcgaggc gtgcttcata tggaatgggc ccgcccggct gcgctccaag
     2461 ctccgggtgc cgaggcgccg tggccgtcgt cacctgtccc gtggccggat cgagccagcc
     2521 gatctgttcc gggtcgcggg tgaagtgccc gttgaaccag atgcgcgagc cgtcgctcga
     2581 cggcgcgatc gagtggggat acatcccgat gtcccagctc gtccagcgcg cgccggccgg
     2641 ttcgtaccgc gccactttca tgggtgcccc gcagagcacc caccagtttc ccgccgcgtc
     2701 gagctcgatg gcccgcgggt tggccttctc gaccgggata ttttccacgc ccaccacgcc
     2761 gctgtcggga ttcacgcggt agatgcgcga cgtgaacatg ccggtcacga gcacgctccc
     2821 gtcggccgtc acggccacgt cgtgcgggag gtcgggtgcc actggcagct ggtactcggt
     2881 gatgcgagcc cggagggcgt cggagtcgcg cggcggcggg gccacgagtg cctcgtacgt
     2941 ggcggcggca tttgcgggcg gcgcggcgcg caggtgactc gacaggaaag cgccgagcga
     3001 ttcggcatcc gcgtcgtgcg aaatgaccgg gaatcccgac tccgatcccg cgaaggcgat
     3061 catgcgcgcc gcgtcggtgc tccactgcac cgccgtccgc agcgaatccc cccggaagac
     3121 gcgcgtctcg ttgagctgat gacagccggt gcagtcgagg atatagcgcc gcttcgtctc
     3181 gccgtcgggg agcgaggcca gccagaccga actcgaccag ccgccgcgtg ccgcgtgacc
     3241 gggattcacc ggccgccagt tcaacgcgag gtcggccgaa tcggcgccgg cgcccgcgtc
     3301 gccgatccgg cgatactcga cgagggcggc acgggcggtc ggtggtgcgg gggaggcggg
     3361 gggcgtggct tgcccggtgg gcgcggagca gccgatgacg aaaaacccga tgaggattcc
     3421 cgcgcgaagc gcggaccaca ggcacttcga catcgtcagg tcagcagacg caggccgaga
     3481 ggaatcaggc gctggaagtc ctttgcatcc gtcgttgcga gtgtcgcttc gctccggatg
     3541 caggaggcgg caatcatgca atcagtcagg ctccgacttc gccgtcccga ctggttgtac
     3601 agcctggccg caagcgaggc gtcttcttcg tggtaggcga cctgttctcg aatcagctct
     3661 ctggccaatt cgacatccgt cggccccacg ggtccgcaca ggaactcggt ccatgcgatg
     3721 gcagtgatcg cgacagactc gccggccgtc agccattccc gaagtctcaa gtcctgtact
     3781 gtatcccgga tcatggctcg aatcaggaaa ctcgtgtcga gatgaatctt catttccgga
     3841 cagtcttgcg gggaaaggcg cgtcgttcgt cgcgaacgcg ctcgacccat tcgtccgctt
     3901 cgacttcagt caaagccagg ctcttctgaa gccgattgag aatcgcgatc ggatcctcct
     3961 cgggcggcgc gggcgcggat gcggcggtca gaataatccg gcgcagggcc tcggaccgcg
     4021 aaaccttcca tcgtttcgcc atgccgtcca gggcacgcaa tgtcttgacg tcgagggaat
     4081 aggtggtctt gacgctcggg gtggccatga ttggacaagg gtaaggcctt acccttgtcc
     4141 ggtcaacgac aaataggcta gaagacgatc ccgaacgtca ccgggatcat ctgcagatcg
     4201 tcctgcccca ggatcgcctt ctcgacgaag acctggtcga tccgcccttc ggcgaagatc
     4261 atcatgcggg ggctgatggg gagcttgagg ccgatcacgc cgttcaggcc aaatcgccac
     4321 ccaggttcga cagggtcgta ttcgaggttg tcctcaacct cacgcgatgg cgcgcggggc
     4381 cgggatcgca cgctttccac ggtttccggc ccgggctcga tactgtgtcc gccggcgccg
     4441 accgcgacgt acggttgaat cgtgccgagc tgaaagtcat gggtgatgtc gagtaacacc
     4501 gacagcgccc ggatggaagc atcgatgcca gaactcgtga agtcgaactc ctgcgctccg
     4561 acgtcgagcc ggaggccgat cggcatcccg gccgaccgat acgagagcat gccgcgagcg
     4621 ttccagccag agtcgtaggt gtccttgaac gtgcctgagg cggggaagct gatgccgcct
     4681 cccatccaga cctgccatgc tttggtcgtc ggggctgccg tactgtccga ttgggcgtgg
     4741 gtggggccgg ccagaacgac gagagcgagt accgccaggg tgacgaaagc aaacgtcgct
     4801 ctcaattcgg cctcgtcaac cgtcgtgaat ggccggagaa atcacccccc gatggcgtgc
     4861 atcggccgat cgggctggtg gaacttcttc ggattgttga tttcgtgccc ttcccact
//
LOCUS       DASVOD010000140        12234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_166436, whole genome shotgun sequence.
ACCESSION   DASVOD010000140 DASVOD010000000
VERSION     DASVOD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12234
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166436"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VF720_02810"
     CDS             <1..759
                     /locus_tag="VF720_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878311.1"
                     /translation="YWGPNGMAFFRNVQFRWMPFKGDSRVTFALERPGASGDQGDYAD
                     RVELSDIQGNFPVPDFSAEARKAGSFGYVELAGIVRRIEWEDLNDDGVDLSDGTTGWG
                     LSGSSNLKLGGKTTARLQAVVGEGIENYMNDAPVDVAIEADDDEEAGFKGVALPMVGI
                     SAFIDHNWSPKFSSSFGYSQLEIDNSDGQSANAFKLGQYALANIMYYPTANVMMGVEG
                     GWIRRENFEDAFDSDNTHVQFSFKYNFSTGIGTR"
     gene            842..2305
                     /locus_tag="VF720_02815"
     CDS             842..2305
                     /locus_tag="VF720_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEX7878312.1"
                     /translation="MGLVALLSGLGAAIAGAQATRTEKDLLGTKQVPADAYYGVQTAR
                     ALENFQLSGTLINQYPGYVEAWAIVKLAAARANTQCGAMKPETLALIEKACKAVIDGK
                     YHDQFIVDWYQGGAGTSTNMNANEVLANVALELGGHKKGEYEIVEPHDDLNMSQSTND
                     SYPTAIKVALMLRNDKLCEELTMLANSFRKKGNEYMTTVKMGRTEMQDAVPMTVGQEF
                     HAFGAALEWEVANLRQAEKALYAENMGATAIGTGINVPKGYAEKCAAELAKLTGKPIV
                     MANDMIAATWDQQGFVIYSAALKSTAIKLSKISSDLILLASGPRAGLFEINLPPLQPG
                     SSIMPGKVNPVMPEVMGIVAMRVMANDFAVTLASHSGQLQLNAYEPLNGLANIESQSL
                     LYNTSKAFRTKCIDGITVNKPTLERYMETTVGIVTALNPVLGYDKATELAAEAYKSGK
                     GILEVIREKKLLTEKQIADILDPVKMSNLDPSIYPKP"
     gene            2328..3392
                     /locus_tag="VF720_02820"
     CDS             2328..3392
                     /locus_tag="VF720_02820"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II asparaginase"
                     /protein_id="HEX7878313.1"
                     /translation="MVMKALRIICTVGLSLLVASGAYAQKKPRIVILATGGTIAGAAS
                     SQTSAGYSSGAVGVDALIAAVPQMKDLADIKGEQIASIGSQDMNDEVWLKLGKRVNEL
                     LASADVDAIVITHGTDTLEETSYFLDLITKSDKPVVMTGSMRPSTAMSADGPLNIYNA
                     VGIAVDPMAKGRGVLVTIDDDIHAAHDVVKTHTTDVGTFTSGEAGLVGGALFGKNVWF
                     RHPEQVHTTKSDLALNNATTLPRVDIIYAHANMSPDIITSAVKSGAKGIVIAGVGDGN
                     MTAPAWAAAKQAVKDGVVVVRSSRTNGGVIRRNIEVNDDEAGTVAAMALNPGKARVLL
                     QLALMKTKDAKAIQGYFDRY"
     gene            3410..4582
                     /locus_tag="VF720_02825"
     CDS             3410..4582
                     /locus_tag="VF720_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014218400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7878314.1"
                     /translation="MRRIRIDLGTGRNFMLKSRGPADWSVRTIFSAIVVLGLAAMEIG
                     PTGAQSRNSRRGSSVPKVTAQFHDSHFHLTNYVQEGITVPQFLKIMGDRVGRSTLFGI
                     PLQQEWSYRVSGNDAPTYYLDTDAGLYYYSFTDAAIAAAYLALPETDRARFDPMITGF
                     NPTDMYAADHIRRVLMTFPGVFSGIGEFTIHKEFVSSKIEGDVASLTDPALDRILDFA
                     AEVGLVVLIHNDIDQPFPKSGQPPRFLAQFRDLVRRHPGNSIIWAHSGVGRIVQPVED
                     HVAMLESMLGDPAFRQMSIDLSWDQVAKYVISTPETTKRAADLIEKYPDRFLFGSDTV
                     APKDNASYFSVYEMYAPLWSLLSESTREKVLKGNYERIFDEGRRRVRAWEKAHVGH"
     gene            4617..5657
                     /gene="glsA"
                     /locus_tag="VF720_02830"
     CDS             4617..5657
                     /gene="glsA"
                     /locus_tag="VF720_02830"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX7878315.1"
                     /translation="MNARFHRGLATSLLAIVAIGASVGTALAQAPKAADVQKALDAAY
                     SKFQNLQEGKNADYIPALAKVDSKIYGIALVTVDGKVYTKGDISSEVSIQSISKVFAM
                     ARVIEDNGPDAILNNMGVDATGQVFNSIVAVEQYKGAEMNAMVNPGAITTTSMIAGGT
                     EDAIWKKIIDTHSDFAGRPLSVNQEVYKSEAETNQRNQAISMLMFAYGHIKSEPLRAC
                     DLYTKQCAISVNAKDLAMMAATLANGGTNPVTGKQCMSPANVPEVLAVMATAGLYDDS
                     GKWLYATGLPGKSGVGGGILAVSPGKFGIAVISPPLDAAGNSVKAQKTIADVSNALGG
                     NPYAPMPMMKKK"
     gene            5743..7065
                     /locus_tag="VF720_02835"
     CDS             5743..7065
                     /locus_tag="VF720_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878316.1"
                     /translation="MDFRRIATRAGLSLLALLSLPVLAFAQDEAAQPDTTRAPENRFI
                     QLPDFAGEFTPAQGFDLLKTKRGSVNLSVYGSIRYLNQMPGDQTFEDHLGRERKVAAR
                     NDLNWHRTFIWVSGFFFDPKFRYTISAWSLPTTEQTLVFGLMRYTIAPALTFGAGVGP
                     SLTARSMQGSWPFWAGSDRQMAEEFYRGGFASSFFATGMPLDRLYYTASVNRSLSQLG
                     ITAANDNRSFAYSGSGWWMPTTGEFGPRSGFGDLEYHQQVATRIGFSTCHAHENRYAP
                     TDDSPRHTQIKLSDGVNPFETGALADSVTVESLDYDNFAVDAGLKYRGFSFQAEYGYR
                     RLSNFEADGAIPEDEIVDHGFFAQAMHMVVPRKLGLYATVGRVFDEFDRDPWEISGGA
                     SFYPYGSRSLRVNLHYIRIDKSPTGSSFGYYTAGQTGDTISMGVDILL"
     gene            complement(7128..8327)
                     /locus_tag="VF720_02840"
     CDS             complement(7128..8327)
                     /locus_tag="VF720_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878317.1"
                     /translation="MSHTRMRKTWPIGPAILVIALASATGRAQSPAEPPPVKADSTAA
                     PTSSGSKFIDPDDGWFDLSAFLDTGHGFVPLVIPITEPAVGYGLAGALVFIDKNPPGP
                     SGGYRRPNIAAVGGLYTENETWAGFAGHSGSWDGDRWQTLVGGIYGSINLDFYGIGEG
                     ELNDEPVAYSLDPAGALGRASYRVGSTPVILGLGYAFANMDVSFTGDVPPGIENRELN
                     ARVAGFTPSITWDTRSNYFTPITGLYASVEPGIYLEALGGTTDFERINTTLLVFQPVG
                     KRICLGLKGEGAFSFGDAPFYTRPFVSLRGVAALRYLGEHAASAELEARWQFYKRLSL
                     VGFVGSGIAWTTFDNLDTQQDVVAGGGGIRYEIARRYGLHMGLDLAFGPDDGVVYVQF
                     GHAWLKP"
     gene            8437..10149
                     /locus_tag="VF720_02845"
     CDS             8437..10149
                     /locus_tag="VF720_02845"
                     /inference="COORDINATES: protein motif:HMM:NF018530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878318.1"
                     /translation="MHHPALMQAIADALRAHTELALFLAVAAGQALGRLRLGSVRMNP
                     VLATLLVGILVGQLGLVIPLAMQYTFFVLFLFSVGFKSGPQFFRTLGPGAVPHVVLTI
                     LLAALSVGVAVWGAHLVGLDAGGSAGLLAGGMSSSTAVGTAGDAITRLSIGEDARHAL
                     QSNLSVVYSLAYICGFITELIVLTRVAPWLMRVNLAEECRKLEKAMGVERTPTGVFSA
                     HREAAVRAYRIPEGFPVRSAVELERMFAPARVFVERIRRGTGIVDAEWNAPIDPGDLV
                     ALTGRHEVLVEPENPLRAHETDDRELLDIPAVIAEVVVTGRWPAGRTLGDLIAENRET
                     PALRSVFIRSITRAGESIPMGPGTVPERGDVISLAGPPSSVSQAAALFGRVEWPSVTT
                     DLISVALAIAVGGLIGIPTLLLFGIHIGLSVPVGVLLAGLVVGWLRSVRPVFGRIPEP
                     ALWVFDTMGLSGFIAIIGINAGPGFVKGFLSLGPAILLASVAIRIIPNVVTLFVGRKL
                     FRLHPGILLGILAGAGCSSAGLAALKEDTQSEVPTLGYGMAYALANVLLGLGATILVT
                     LLSR"
     gene            10340..11617
                     /locus_tag="VF720_02850"
     CDS             10340..11617
                     /locus_tag="VF720_02850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7878319.1"
                     /translation="MKRKPLLIGSVLLVLVASGAGFVLLRGRGEKPLEVQTAAVARQK
                     IVQKVNGTGKIQPKTRVEISADVSARIIALPIVNGQWVEKGTFLVGLDRERYLAAVEN
                     AEAAVSSAEAGAAVVKENMVRTENEFKRSKELQASGMESQAMFENKQAEHQVEVARYK
                     SALDQVDQAKAVLKQARDDLSKTSIYAPMSGIISALNKETGEIALGSQFQKDVILVIA
                     DLKEMEAQVNVDENDIVSLAVGQAAEIEVDALPDQKLAGIVSEIAISANSAGAGSTDQ
                     KTEFEIRINITNPPSSLRPGMTAGADIVTKTNDNAVGVPIQSVAVRTVDQLTTKGESR
                     EDAEKRFKADKDGFVEVVFCIDGEKAMARQVTTGIQSDEYIEIVDGLAEGDQIVTGSY
                     RAISKDLVNGAVVAIAKAPPGKPKEAPATAEAR"
     gene            11622..>12234
                     /locus_tag="VF720_02855"
     CDS             11622..>12234
                     /locus_tag="VF720_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878320.1"
                     /translation="MRAPTLSLRNVVKTYEVGGETTRALAGVSLDIEANEYVAIMGPS
                     GSGKSTLMNMVGCLDVPTSGEYLLEGEAVSGMSEVRLADIRNRRVGFVFQTFNLIPRA
                     DILHNVELPLVYAGVPPAERRQRAMKALEVVGLADRMHHRPNELSGGQRQRAAIARAL
                     VLDPSIILADEPTGNLDTKTSAEVMAVFDGLHAAGQTIILVTHE"
BASE COUNT         2260 a         3966 c         3974 g         2034 t
ORIGIN      
        1 tactggggcc ccaacggcat ggcgttcttc cgtaacgtgc agttccgctg gatgcccttc
       61 aagggcgatt cgcgcgtcac gttcgccctc gagcgaccgg gtgccagcgg cgaccagggc
      121 gactacgccg accgcgtcga actctccgat atccagggga acttcccggt gcccgatttc
      181 tccgccgagg cccgaaaagc cggcagcttc ggttacgtcg agctggccgg catcgtccgc
      241 cggatcgagt gggaagacct gaacgacgac ggggtggatc tctccgatgg caccaccggc
      301 tggggcctct cgggcagctc aaatctcaaa ttgggcggca agacgaccgc ccgcctccag
      361 gccgtcgtcg gcgagggtat cgagaactat atgaacgacg cgccggtcga cgtggccatc
      421 gaggccgatg acgacgagga agccgggttc aagggcgtgg cgcttccgat ggtcggcatc
      481 tcggccttca tcgaccacaa ctggagcccg aagttctcga gctcgttcgg ctactcgcag
      541 ctcgagatcg acaactcgga cggccagtcg gccaatgcct tcaagctcgg ccagtacgcg
      601 ctggccaaca tcatgtacta cccgaccgcc aacgtgatga tgggggtgga gggtggctgg
      661 atccgtcgcg agaacttcga ggacgccttc gacagcgaca acacccacgt ccagttctcg
      721 ttcaagtaca acttctccac gggcatcggc actcgctgag cccggctttt ccgatcaccc
      781 cagggtttcc tggaaaggac acgaggtcat gaaaaggcac cgtccggacg cgctgcgcgt
      841 catgggtctg gttgcgctgt tgtcagggct gggagccgca atcgccggcg cccaggccac
      901 gcgcaccgaa aaggatcttc tcgggacgaa gcaggtcccg gcggatgctt actacggtgt
      961 gcaaacggcc cgtgcgctcg agaatttcca gctttcagga accctgatca atcaataccc
     1021 cggttacgtg gaggcgtggg cgatcgtcaa gctggcggcc gcccgcgcca acacgcagtg
     1081 cggcgccatg aagcccgaga cgctggccct catcgagaag gcctgcaagg cagtcatcga
     1141 cggcaagtat cacgaccagt tcatcgtgga ctggtaccag gggggcgccg gcacctccac
     1201 gaacatgaac gccaacgaag tcctggcgaa cgtggctctc gagctcggcg gccacaagaa
     1261 gggcgagtac gagatcgtcg agccgcatga cgacctcaac atgtcgcagt cgaccaacga
     1321 ctcgtacccg acggccatca aggtggccct gatgctccgg aacgacaagc tgtgcgagga
     1381 gctcacgatg ctcgccaact cgttccgcaa gaaggggaac gagtacatga ccaccgtgaa
     1441 gatggggcgc accgagatgc aggacgcggt gccgatgacg gtgggccagg agttccatgc
     1501 gtttggcgcc gccctggagt gggaggtcgc caacctccgg caggccgaga aggcgctcta
     1561 tgcggagaac atgggcgcga cggccatcgg cacgggtatc aacgtgccga aggggtatgc
     1621 cgagaagtgc gccgccgagc tggccaagct gaccggcaag ccgatcgtca tggccaacga
     1681 catgatcgcc gccacctggg accagcaggg tttcgtcatc tattccgcgg cgctcaagag
     1741 caccgccatc aagctctcga agatctcgag cgacctgatc ctgctggcgt ccggcccacg
     1801 ggccggcctc ttcgagatca acctgccgcc gctccagccg ggctcctcga tcatgcccgg
     1861 caaggtcaac ccggtcatgc cggaggtgat ggggatcgtg gccatgcgcg tgatggccaa
     1921 cgacttcgcg gtgacactgg cctcgcacag cggccagctc cagctcaacg cctacgagcc
     1981 gctcaacggc ctggccaaca tcgagtcaca gtcgctcctc tataacacgt cgaaggcatt
     2041 tcgcacgaag tgcatcgacg gcatcaccgt caacaagccg acgctcgagc gctacatgga
     2101 gacgacggtc ggcatcgtga ccgcgctgaa ccccgtgctg ggctacgaca aggccaccga
     2161 actggcggcc gaggcataca agagcggcaa ggggatcctc gaggtcatcc gcgagaagaa
     2221 gctgctgacc gagaagcaga tcgccgacat tctcgacccg gtgaagatgt cgaatctcga
     2281 cccctcgatc tacccgaagc cctgaccgac cggtcagaaa aggagtcatg gtcatgaaag
     2341 ccctcagaat catctgcacg gtcggcctca gcctgctcgt ggcgagcggt gcgtatgccc
     2401 agaagaagcc gcggatcgtt atcctggcca ccggcggcac cattgccggc gcggcctcct
     2461 cgcagacgtc ggccggctac tcgtccggag ccgtgggcgt ggacgcgctg atcgccgccg
     2521 tcccgcagat gaaggacctg gccgacatca agggcgagca gatcgcctcg atcggaagcc
     2581 aggacatgaa cgatgaggtg tggctcaagc tgggcaagcg ggtgaacgag ttgctggcct
     2641 ccgcggatgt cgacgccatc gtcatcacgc acggcaccga cacgcttgag gaaacctcct
     2701 acttcctcga cctgatcacc aagagcgaca agccggtggt catgaccggt tcgatgcgtc
     2761 cctccaccgc catgagcgcc gacggcccgc tgaacatcta caacgcggtg ggcatcgctg
     2821 tggatcccat ggccaagggg cgcggcgtgc tcgtcacgat cgacgacgac atccatgcgg
     2881 cccacgacgt ggtcaagacg cacacgacgg acgtcggcac attcacctcc ggcgaggccg
     2941 gcctggtggg cggcgcgctc ttcggcaaga acgtctggtt ccgtcacccc gagcaggtgc
     3001 acaccacaaa gagcgacctg gccctgaaca acgccacgac gctgccgcgg gtcgacatca
     3061 tctatgcgca cgccaacatg tcgcccgaca tcatcacctc ggccgtcaag agcggcgcca
     3121 aggggatcgt gatcgccggt gtcggcgacg gcaacatgac cgcccctgcc tgggccgccg
     3181 ccaagcaggc cgtgaaggat ggggtggtcg tcgtccgctc ctcgcgcacc aacggcggcg
     3241 tcatccgccg gaacatcgag gtgaacgatg acgaggcggg aacggtggcg gccatggcct
     3301 tgaacccggg caaggcgcgc gtgctgctcc agctggcgct catgaagacg aaggacgcga
     3361 aggcgatcca gggctacttc gatcgttact gatcggaaca ctcgaaacca tgcgacgcat
     3421 ccgaattgac ctgggaacgg gtcggaattt catgctgaaa tcgcgcgggc cggccgactg
     3481 gtcggtccgc acgatttttt cggccatagt ggtcctgggc ctggccgcga tggagatcgg
     3541 accaaccggg gcccagtcac gaaactcgcg ccggggctcg tcggtcccga aagtgaccgc
     3601 ccaattccac gactcgcact tccacctgac caactacgtc caggaaggga tcaccgtccc
     3661 gcagttcctc aagatcatgg gcgatcgtgt cgggcgctcc acgctcttcg ggatcccgct
     3721 tcagcaggag tggtcgtacc gggtgagcgg caatgatgcg cccacctact acctggacac
     3781 tgacgccggc ctctactact actcgttcac cgacgcggcg atcgcggcgg cctacctggc
     3841 gcttccggag acggaccgtg cgcggttcga cccgatgatc accggcttca acccgaccga
     3901 catgtacgcg gcggaccata tccgccgggt gctcatgacc tttcccggcg ttttcagcgg
     3961 catcggggag ttcacgatcc acaaggagtt cgtctcctcg aagatcgagg gtgacgttgc
     4021 gagcctgacc gacccggccc tcgaccggat cctcgacttc gcggccgaag tgggcctcgt
     4081 ggtcctcatc cacaacgaca tcgaccagcc gttcccgaag agtggccagc cgccccgctt
     4141 cctggcgcag ttccgcgacc tggtgcgccg gcatccgggc aactcgatca tctgggcaca
     4201 cagcggcgtg gggcgtatcg tgcagccggt cgaagatcat gtcgccatgc tcgagtcgat
     4261 gctcggtgat ccggccttcc ggcagatgtc gatcgacctg tcgtgggacc aggtggccaa
     4321 gtacgtgatc tcgacacccg agaccacgaa gcgggccgcc gacctgatcg agaagtaccc
     4381 cgaccgtttc ctgttcggct cggacaccgt ggcccccaag gacaatgcct cctatttttc
     4441 ggtgtacgag atgtatgcgc cgctgtggag cctgctttcc gaatccacgc gcgaaaaagt
     4501 gctgaagggc aattacgaac ggatcttcga cgagggtcgc cgccgggttc gcgcctggga
     4561 aaaggcccac gtcggtcatt gaatcaatcc atcgtctgag agaccaggag gaacacatga
     4621 acgcgcgttt tcatcgggga cttgccacca gcctgctcgc gatcgttgcc atcggcgcat
     4681 cggtcgggac ggccctcgcc caggccccga aggctgccga cgtccagaag gcgctggatg
     4741 ccgcctactc caagttccag aacctgcagg aagggaaaaa cgccgactac atcccggcac
     4801 tggccaaggt cgattcgaag atttacggca tcgcgctcgt gacggtggac ggcaaggtgt
     4861 acacgaaggg ggacatctcc tccgaggtgt cgatccagtc gatctccaag gtgttcgcga
     4921 tggcgcgtgt gatcgaggac aacggcccgg acgccatcct gaacaacatg ggtgttgacg
     4981 ccaccggcca ggtgttcaac tcgatcgtgg ccgtcgagca atacaagggc gcggagatga
     5041 acgcgatggt gaaccccggt gcgatcacga ccaccagcat gatcgccggg ggcaccgagg
     5101 acgccatctg gaagaagatc atcgacacgc acagcgactt cgccgggcgt ccgttgtcgg
     5161 tgaaccagga ggtctacaag tccgaagcgg agacgaatca gcggaaccag gccatttcga
     5221 tgctgatgtt cgcctacggc cacatcaagt ccgagcctct ccgcgcatgc gatctctaca
     5281 cgaagcagtg cgccatcagc gtgaacgcca aggacctggc catgatggcc gctaccctgg
     5341 ccaacggtgg cacgaatccg gtcaccggca agcagtgcat gagcccggcc aacgtgcccg
     5401 aggtgctggc ggtgatggcc accgccggtt tgtacgacga ttcgggcaag tggctctacg
     5461 cgaccgggct tccgggcaag agcggcgtgg gcggcggcat cctggccgtc tcgccgggca
     5521 agttcggcat cgccgtcatc tcgccgccgc tcgacgcggc cggcaacagc gtaaaggcgc
     5581 agaagaccat tgcggacgtg tccaacgcgc tcggcggaaa tccctacgcg ccgatgccga
     5641 tgatgaagaa gaagtaggca ggccgtactt ccggtacaca agcaagggcc cggccgcaca
     5701 ggtcgggcct ttgcatctgc actcctcacg aaaggagacc gattggactt tcgtcgcatt
     5761 gcaacacgtg cgggcctgag cctcctggcc cttctgtcgc tgccggtcct ggcgtttgcc
     5821 caggacgagg ccgcccaacc cgacacgacc cgggcgccgg agaaccgctt catccagctg
     5881 ccggacttcg ctggtgagtt cacgcctgcc cagggcttcg atctgctgaa gacgaaacgc
     5941 ggcagcgtca acctcagcgt atacggcagc attcgctatc tgaaccagat gcccggtgac
     6001 cagacattcg aggaccacct gggaagagag cgcaaggttg cggcccgcaa cgatctcaac
     6061 tggcaccgaa cgttcatctg ggtgagcggc tttttcttcg acccgaagtt ccggtacacg
     6121 atctcggcct ggtcacttcc taccaccgag cagactctgg ttttcggcct gatgcgttac
     6181 acgatcgcac cggcgctcac gttcggcgct ggtgtcggac ccagcctgac cgcccgttcc
     6241 atgcagggct cctggccttt ctgggcgggg agtgaccgcc agatggccga ggagttctac
     6301 cgtggcggct ttgcttcatc gttcttcgcg accggcatgc ccctcgaccg cctctactac
     6361 acggcatcgg tcaaccggag cctgagccag ctcggcatta cggcggctaa cgacaatcgt
     6421 tcgtttgcct acagcggcag cggatggtgg atgccgacga ccggggagtt cgggccgcgg
     6481 agcggtttcg gggacctgga gtatcaccag caggtcgcca cccggatcgg gttttcgacc
     6541 tgtcacgccc acgagaatcg ctacgcgccc actgatgatt cgccgcgaca cacccagatc
     6601 aagctgtcgg acggcgtgaa cccattcgag accggagccc tggcggatag cgtcaccgtc
     6661 gaatcgctcg actacgacaa ctttgccgtg gatgccgggt tgaagtaccg cgggttctcg
     6721 tttcaggcgg agtacggcta ccgtcgtctg tcgaacttcg aggcagacgg tgccattcca
     6781 gaagacgaga tcgtggacca tggattcttc gcccaggcaa tgcacatggt ggttccgcgc
     6841 aagctcgggt tgtacgccac cgtcggccgt gtttttgacg agttcgaccg cgatccgtgg
     6901 gaaatctcag gtggcgcgag cttctatccg tacggtagcc gctcgctccg cgtgaacctc
     6961 cactacattc gcatcgacaa gtcgccgacc ggttccagct tcgggtacta caccgcgggc
     7021 cagaccgggg acacgatctc gatgggggtc gatatcctgc tgtaggtggt atcgatcaag
     7081 gctcgaaatg cgacggggct cctcctggtg gggagccccg tcgtgcttca tggcttgagc
     7141 caggcgtggc cgaactgcac gtacacgacg ccgtcatccg ggccgaaggc caggtcgagc
     7201 cccatgtgca ggccgtagcg gcgggcgatc tcgtagcgga tgccgccgcc gccggccacg
     7261 acgtcctgct gcgtgtcgag gttatcgaac gtggtccagg cgatgccgga cccgacgaat
     7321 ccgaccaggc tcagccgctt gtagaactgc cagcgcgcct cgagttcggc gctggcggca
     7381 tgctcgccga gatagcggag cgccgccacg ccgcgcagcg acacgaacgg ccgggtgtag
     7441 aagggcgcat cgccgaagct gaaggcgccc tcgcccttca accccaggca gatccgcttg
     7501 ccgaccggct ggaagaccag gagcgtggtg ttgatccgct cgaagtcggt cgttccgccg
     7561 agcgcctcga ggtagatgcc cggctcgacg ctcgcgtaca ggcccgtgat cggcgtgaag
     7621 tagttgctgc gggtgtccca ggtgatcgac ggggtgaagc cggccacgcg cgcgttgagc
     7681 tccctgttct cgatgccggg cggcacgtcg ccggtgaagg acacgtccat gttggcgaag
     7741 gcgtagccca ggccgaggat cacgggggtg ctgccgacgc ggtagctggc ccgcccgagc
     7801 gcgccggccg ggtcgagtga ataggcgacg ggctcgtcgt tcagctctcc ctcgccgatg
     7861 ccgtagaaat cgaggttgat cgagccgtag atgccgccca ccagcgtctg ccagcggtcg
     7921 ccatcccagg agcccgaatg gccggcgaaa ccggcccacg tttcgttctc cgtgtagagc
     7981 ccgcccacgg cggcgatgtt gggccgccga tagccgccgg aaggccccgg cggattcttg
     8041 tcgatgaaga cgagcgcgcc cgccaggccg tatccgacag ccggctcggt gatcgggatg
     8101 acgaggggga cgaacccgtg cccggtgtcg aggaaggcgc tgagatcgaa ccacccgtcg
     8161 tcggggtcga tgaatttcga acccgacgag gttggcgcgg cagtggaatc ggccttcacg
     8221 ggaggcggtt ccgcgggtga ctgcgcgcgg ccggtggccg aagcgagggc gatgacaagg
     8281 atcgccgggc cgatcggcca cgtcttccgc attcgggtat ggctcatgac cgtgggcacg
     8341 tcggcagaat aaggacgagg cgcggccacg tccagacgaa gacgatcccg gcgcgaggtt
     8401 ccgtgacgcg caccactccc ggacacgccc gttttcgtgc atcatcccgc actcatgcag
     8461 gcaatagcgg atgcactgcg cgcacacacc gagctggctc tcttcctggc ggtggctgcc
     8521 ggccaggccc tggggcggtt gcgactcggt tccgtgcgga tgaacccggt gctcgccacg
     8581 cttctcgtgg ggatcctggt cgggcagctc ggcctcgtga ttccgctcgc catgcagtac
     8641 accttctttg tcctcttcct cttcagcgtc ggattcaaga gcggcccgca gttcttccgc
     8701 accctgggcc caggggccgt tccgcatgtc gtcctgacga tcctcctcgc cgcgctttcc
     8761 gtcggcgtcg ccgtatgggg agcccacctg gttggattgg atgccggcgg ctcggccggt
     8821 ctcctggcgg gagggatgag ttcctcgacc gccgtcggca ccgccggcga tgcgatcacg
     8881 cggctgtcga tcggggaaga tgctcggcac gcgctccagt caaacctgtc ggtcgtctac
     8941 tcgctcgcct acatctgcgg attcatcacg gaattgatcg tgctgacacg cgtggcgcca
     9001 tggctcatgc gcgtcaacct ggccgaggag tgccggaagc tcgagaaggc gatgggcgtg
     9061 gagcggaccc cgaccggagt cttttcggcc catcgcgaag ccgccgtccg ggcctaccgg
     9121 atccccgagg gctttcccgt ccgttccgcg gtcgagctcg aacgaatgtt cgcgccggcc
     9181 cgcgttttcg tggaacggat acgccgcggc accgggatcg tggatgccga atggaacgca
     9241 cccatcgacc ccggcgacct cgtggccctg accggtcgtc acgaagtgct ggtggagccg
     9301 gaaaatccat tgcgcgcgca cgagacggac gaccgggagc tgctcgacat cccggccgtg
     9361 atcgccgagg tggtggtcac cggcaggtgg cccgccggcc gcaccctggg ggatttgatc
     9421 gcggagaacc gcgagacgcc ggcgctgcgc agcgtcttca tccggagcat cacccgcgcg
     9481 ggggaatcga tcccgatggg gcccggcacc gtgccggagc gcggcgacgt gatctcgctg
     9541 gctgggccgc cctcgtccgt ctcgcaggcg gcggcgctct tcggccgggt cgaatggcct
     9601 tcggtgacga ccgacctgat ctcggttgcg cttgccattg cggttggggg gttgatcggc
     9661 attcccacgc tcctgctctt cggcatccac atcggcttga gcgtgccggt cggcgtgctc
     9721 ctggcggggc tggtcgtcgg ctggctccgg tccgttcgcc ccgtcttcgg ccggattccg
     9781 gagccggcac tctgggtgtt cgacaccatg gggctcagcg gcttcatcgc gatcatcggc
     9841 atcaatgccg gtccgggatt cgtgaaaggt ttcctgagcc ttggaccggc catcctcctg
     9901 gcctccgtgg ccatccggat cattccgaat gtcgtgaccc tcttcgtcgg acgaaaattg
     9961 ttccggctgc atccgggtat cctgctggga atccttgccg gggccgggtg ctcctcggcc
    10021 ggtctggccg ccctcaagga agatacccag agcgaggtgc cgaccctcgg atacggaatg
    10081 gcctacgccc tggccaacgt cctcctggga ctcggggcca cgatcctcgt gaccctcctc
    10141 tcccggtgac caataatgag tatcagtcac ccggatcgga ccggccgttg cccgctgtaa
    10201 taaagttgta acgcggtccg cctcggggcg caccgcattg aacagaaacc cgcaacctcc
    10261 gtctgtaact tctcgtatcc aacctgccgc acgccgtcac attgcctaca ggcccaagtc
    10321 tcaagggggg cgtttgccca tgaaaaggaa gccgcttctg atcgggtcgg tgctgctggt
    10381 cctcgtggcc tccggtgccg gcttcgtgct cctccggggg cgaggggaaa agcctctcga
    10441 ggtacagacc gccgccgtgg cccgccagaa aatcgtgcag aaggtgaacg gcaccgggaa
    10501 gatccaaccg aagacgcgcg tggagatcag cgccgacgtg agcgcgcgga tcatcgccct
    10561 gccgatcgtg aacgggcagt gggtggagaa ggggacattc ctggtgggcc tcgatcggga
    10621 acgttacctg gcggccgtgg aaaacgccga ggccgccgtg agctccgccg aggcgggcgc
    10681 cgccgtcgtc aaggagaaca tggtccgcac cgagaacgag ttcaagcgtt cgaaggagct
    10741 ccaggccagc gggatggaat cgcaggccat gttcgagaac aagcaggccg agcaccaggt
    10801 ggaggtggcc cgctacaagt cggctctgga tcaggtggac caggcgaagg ccgtgctcaa
    10861 gcaggccagg gatgacctgt ccaagacaag catctacgcg ccgatgtcgg ggatcatcag
    10921 cgccctcaac aaggaaaccg gcgagattgc gctcggctct cagttccaga aggacgtcat
    10981 cctcgtcatc gccgacctga aggagatgga agcccaggtg aacgtggacg agaacgacat
    11041 cgtctcgctg gccgtcggcc aggcggccga aatcgaggtc gatgcactgc ccgaccagaa
    11101 actggcgggc atcgtctcgg agatcgccat cagcgccaac tcggcgggag cgggctcgac
    11161 cgaccagaag accgagttcg agatccgaat caacatcacg aacccgccgt cgtcgctccg
    11221 gccgggaatg accgccggcg cggacatcgt gacgaagacc aacgacaatg ccgtcggcgt
    11281 gccgatccag agcgtggccg tgcgaaccgt tgaccagctc accacgaagg gggagtcgcg
    11341 cgaggacgcc gagaaacgct tcaaggccga caaggacggc ttcgtcgaag tcgtgttctg
    11401 catcgacggg gagaaggcga tggctcgaca ggtcacgacc ggcatccaga gcgacgagta
    11461 catcgagatc gtggatggac tggccgaggg agaccagatc gtgaccggga gctaccgggc
    11521 catctcgaag gacctggtga acggcgcggt cgtggcgatc gcgaaggccc cgcccggcaa
    11581 gccgaaggaa gcccccgcca ccgccgaggc ccggtagttt cgtgcgcgcg ccgaccctgt
    11641 cgctccgcaa cgtcgtcaag acgtacgaag tgggtggcga aacgacgcgc gcgctggccg
    11701 gcgtgtccct tgacatcgag gccaacgagt acgtggccat catggggccg tcggggtcgg
    11761 ggaagtccac cttgatgaac atggtcggct gcctcgacgt gccgacctcg ggcgaatacc
    11821 tcctcgaggg tgaggccgtc tcggggatgt ccgaagtgcg actggccgat atccggaacc
    11881 ggcgcgtcgg cttcgtcttc cagacgttca acctgattcc gcgcgccgac atcctgcaca
    11941 acgtggaact gccgctggtg tacgcgggcg ttccgccggc cgagcggcgc cagcgggcca
    12001 tgaaggcgct cgaggtggtc gggttggccg accggatgca tcaccgcccc aacgagcttt
    12061 cgggtggcca gcggcagcgg gccgccatcg cgcgggccct ggtcctcgat ccatcgatca
    12121 tcctggcgga cgagccgacc ggtaacctcg acacgaagac cagcgcggaa gtgatggcgg
    12181 tgttcgatgg gctccacgcc gccggccaga cgatcatcct cgtgacgcac gagg
//
LOCUS       DASVOD010000141         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_635001, whole genome shotgun sequence.
ACCESSION   DASVOD010000141 DASVOD010000000
VERSION     DASVOD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635001"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VF720_02860"
     CDS             complement(<1..566)
                     /locus_tag="VF720_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878321.1"
                     /translation="MSKNDGAAWGSSLHDGHRNDMLGGDCETCHGSSEYPVRLDSSLG
                     GTGFSPIGCLGCHGRAEPAAGGAVTGAGLRQHHYRTGVTTCATAGCHTDANPAAFVAF
                     GENVKPPYYFTPDAAHPDKPTSTCNIGGSEGAVAPPLGLDNDGDNLYDQADHDCRLTG
                     VPDAPDVTGLALAAVAPNPVVRRGRFHVT"
     gene            992..1621
                     /locus_tag="VF720_02865"
     CDS             992..1621
                     /locus_tag="VF720_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX7878322.1"
                     /translation="MNKYVTELIGTFFLVLTIGCTVLSRGTGIMPPLAIGSMLMVMIY
                     AGGHISGAHFNPAVTLAVVMRGKCPSKDMLPYILFQLAGAALAAMVVRFLIPDAAATP
                     MTLDVGHALLAEFLFTFALAWVVLNVATAKANSGNSFYGLAIGFTVLAGAFAVGPISG
                     GAFNPAVALGAALMGLLDLAQIWIHVAGNFAGAAVAALAFKSLSTADAA"
BASE COUNT          231 a          577 c          570 g          297 t
ORIGIN      
        1 gtcacgtgga accgcccgcg gcgcacgacg ggattggggg ccaccgccgc cagcgccaga
       61 cccgtcacgt ccggcgcatc cgggaccccg gtgagacggc agtcatggtc ggcctggtcg
      121 tagaggttgt cgccgtcatt gtccaggccg aggggcgggg ctaccgcacc ctccgagccc
      181 ccgatgttgc aggtggaagt cggcttgtcg gggtgtgccg catccggggt gaagtaatag
      241 ggcggcttga cgttctcccc gaacgccacg aaggcggcgg gattggcgtc ggtgtggcag
      301 ccggcggtgg cgcacgtggt cacgccggtt cgatagtgat gctggcggag ccccgcgccc
      361 gtcacggcgc ctcccgccgc cggctcggcc cggccatggc aaccgaggca gccgatcggg
      421 ctgaagccgg tcccgccgag cgatgaatcg aggcggaccg ggtattcgct gctcccatgg
      481 caggtttcgc agtccccgcc gagcatgtcg ttgcggtggc catcatggag gctggagccc
      541 cacgcggctc cgtcgttctt cgacacgtaa ttgccgacac ggaatccccc gtggcagtcg
      601 gcgcaattcc cggccgttct gctgagggag taccggtcat accccgcggc cgggggcacc
      661 agggcgacga tggcggccgc cgccaggacg agccggacaa acgatccgga tgttcgaagt
      721 gttggggttc ggtccgtggc ggacagttgg cgtggagttc tcatcatctc tccttgtgac
      781 gcccgttcgg gcggggtgac gatccgtcac gcgaccaccc gctgacgctg cgcccggtgg
      841 gcgccgcctg ccatgacaat tctcgggacc gaattgagaa atcgaccgga tcctgtcgcc
      901 gagccgggct ctttccaccc gccttcgcgc ccgctacagt ccccgcgtcc gttcctcgcg
      961 tgccctggcc gacccgttgc aaggaggagt cgtgaacaaa tacgtcaccg aactgatcgg
     1021 aacgttcttc ctcgtgctca ccatcggatg caccgtcctg agccgcggta cggggatcat
     1081 gccgccgctc gccatcggtt cgatgctcat ggtgatgatt tacgcgggcg gccacatcag
     1141 cggcgcccac ttcaatccgg cggtgacatt ggccgtggtc atgcgcggca agtgcccgtc
     1201 gaaggacatg ctgccgtaca tcctctttca gctggccggc gcggcactgg ccgcgatggt
     1261 cgtcaggttc ctcattccgg atgcggccgc cacgccgatg accctcgatg tcggccacgc
     1321 gctcctcgcc gagttcctgt tcacgtttgc cctggcttgg gtggtgctga acgtggccac
     1381 ggcgaaggcc aacagcggaa actcgttcta cggcctggcc atcggtttca ccgtcctggc
     1441 cggcgcgttc gcggtgggcc cgatctcggg tggcgcgttc aatcccgccg tggcgctcgg
     1501 ggccgcgctc atgggcctgc tcgacctcgc gcagatctgg atccacgtgg cgggcaactt
     1561 cgccggcgcg gcggtggccg ccctggcctt caagtcgctg agcacggcgg acgccgcctg
     1621 acgctcgacg ccactttcgc ccttcgcctc gccgccgtcc gcgcgcggat ccagg
//
LOCUS       DASVOD010000142         7086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_322086, whole genome shotgun sequence.
ACCESSION   DASVOD010000142 DASVOD010000000
VERSION     DASVOD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322086"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VF720_02870"
     CDS             <1..872
                     /locus_tag="VF720_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7878323.1"
                     /translation="PLPVLVNQAAGSLSRASPADDLPGAAESAGIPIEIMVCDGPGVM
                     RQTVCRLRDQGAGTIAVAGGDGTVALVAQELAHTTVDLGIIPAGTANNFATALGLPRD
                     WREALEVVRDGGTERVDLGKAGERYFTESAGVGLFADSLEYYGRRTGKHFGKLVYAAG
                     RLLMSARARRMKLTIDGEQVVERAVMCEVANTYRMGLGAPVAPEANPADGWLDVVLIG
                     DLSRWELLRYYRAVMAERHLELPKVKATRARRVTIEALHPMNVHCDDRIIGTTPAEFS
                     VARAALRVRVPRS"
     gene            869..1534
                     /locus_tag="VF720_02875"
     CDS             869..1534
                     /locus_tag="VF720_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7878324.1"
                     /translation="MRRWLVMPLGYLSSARQSVAFWVMLAMFLGFLTLSLLVRSPGLT
                     QRDAMLTRAIQHWRTPGLDSFFRAVTFLGDPIPPLLVAALASAWLFRMGQQWPGVFVL
                     ISTAGLPLNFLIKELIRRPRPAGDSVNVLLDVVGRSFPSGHAMTATMVYGFLAYLGFV
                     LPARPLHRALLVALFTILPLLIGFSRVYLGVHWASDILGGWTAGLFFLFLMVLAYRLV
                     TAV"
     gene            complement(1536..2357)
                     /locus_tag="VF720_02880"
     CDS             complement(1536..2357)
                     /locus_tag="VF720_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018622605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7878325.1"
                     /translation="MTLRRTLTNGLALAVLLGSTVLPTGCSKVPVTGRQQLNLIPSST
                     ILSMSNDEYDTFLKEHRLSGNTTQVQLVRRVGSRIQQAVETYFRQHDLSSQLDGYAWE
                     FNLVEDKAANAWCMPGGKVVVYTGILPVTQTEAGLAVVMGHEIAHAVAEHGNERMSQG
                     LLTQLGGLALSEALSSKPAQTRQIWMTAFGVGTQVGALLPFSRLQESEADRLGLIFMA
                     MAGYNPNEAVSFWERMSANSSGAPPEFLSTHPSDRTRIANLQKEIPEAMRYYNQS"
     gene            2743..3573
                     /locus_tag="VF720_02885"
     CDS             2743..3573
                     /locus_tag="VF720_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878326.1"
                     /translation="MRTIDTDRQNELHELHVVRPENHGTRNALLIGGAILVLAIGGAL
                     ALQGNNNLPEGEMAGNVSSPAPVTPGASTSGSIATPRPPAVATTPTPARPRPAASNLV
                     TIPQGTPVTVELRNEIGTKHSRVGDTFTAVVAQSVSANGRTAIPAGSTVNGRVILSEQ
                     PGKASGRGKLQLQFNTVSINGRSYDLGSRSAVFESKSGTEKDVAIIGGGAAAGGVAGA
                     ILGKDAGSAAKGAAIGGAAGTVASLLTRGPQLTLEPGTNIRFTLDQPVAVRPPSNTNS
                     "
     gene            complement(3684..5606)
                     /locus_tag="VF720_02890"
     CDS             complement(3684..5606)
                     /locus_tag="VF720_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878327.1"
                     /translation="MRPTPQARSTSRHSTLLVLVLALVAGSCGGTPDPTGRSAQMAVA
                     SALARQLAPHVAAGERRTVASIIEDAMARPDVAWVIVQDTDGQSVASVQRGDVDREAA
                     RTAIVRGAWCPTTATWTECRATAPSVVHNIGIPIPGAGATDAPILGTLNLGFRDARVG
                     IPFSGLIQRGRRNLGALPATFGSQVTTASSQARRYYDDGIEAIRKFYDNEARTAWMMA
                     LEEDPHLAMAMVRLGRLAHDLADDEGALDWYRRATIEEARVTDRERLEIRLLGADLEG
                     RESDERALEDELIRRFHDDADVLILAANQHLSEGRPDEALSELQRAEEIDPARTDVWN
                     LLGYARSELGSWPEAEDAFERYVFVHEQQANPHDSLGEFYLRTGRYRKALAQFETACE
                     IKPDFSWAHYHLALVNAELGRWEASLFAIQRARDISGESPEGRQWSRTQIALHLRAGR
                     IAEAASLAEVHCSAYALKPADQALLARVALAVGDRAGTRQYLDAMLLALQQQARARGC
                     DLTEVDNYYNLPELEALLLLGAGSPREAVDLLDRALPAAHHWEARQRIERETIGALID
                     SKRPDEALRRLEKVLARNPNDPAANWSFARILESRGEVQEARAALARARQALEGADED
                     DPLTAAVREASRGTGG"
     gene            5957..6511
                     /locus_tag="VF720_02895"
     CDS             5957..6511
                     /locus_tag="VF720_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7878328.1"
                     /translation="MNDQSLISRILAGDVGAERQLYDAHVDRIYRLACRMTGDPLHAE
                     ELTQESFIRAFSRLATFRGDAAFSTWMHQVALSVIISGLRKKKRIRERETSLETMTNF
                     DPGRPAERHELKRKLKTAVDDLSEILKTVLVMYEVEGYSHGEIASILEIPEGTSRARL
                     ARAKEILRGVLEPPKIVGLEGELR"
     gene            6508..>7086
                     /locus_tag="VF720_02900"
     CDS             6508..>7086
                     /locus_tag="VF720_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878329.1"
                     /translation="MSDRQFDDLLKETLEEYGEPGATPREAMWRSVSESRRKVVSLPW
                     WRRPMTRVDFLAGAVAAAVLILVGILVGRMTQNPPTPTGRTTPSFTAESNPAPSAPVE
                     SIDRPVPDGIDRGTAVNTRKPVRHDVVRTPSPSLGELAKVPGQAEGSDAPLPSVASGE
                     LEIGMRSQPGDAEREVLRLAATRYLSRTESFLT"
BASE COUNT         1141 a         2370 c         2333 g         1242 t
ORIGIN      
        1 atcccctccc ggtcctcgtg aaccaggcgg cgggttccct gagccgcgcc tcaccggcgg
       61 acgacctgcc gggcgccgcc gagtccgcgg gcattccgat cgagatcatg gtgtgcgatg
      121 gtcccggtgt catgcgccag accgtgtgcc gcctgcggga ccagggggcg ggcaccatcg
      181 ccgttgcggg aggggatggc accgtggcac tggtggccca ggagctcgcc cacaccaccg
      241 tggacctcgg gatcattccg gctggcaccg ccaacaactt cgccacggcg ctcggcctcc
      301 cgcgcgactg gcgcgaggcg ctggaggtcg tgcgcgacgg cggcacggag cgcgtcgacc
      361 tgggcaaggc aggggaacgc tacttcaccg agtcggccgg cgtcggcctg ttcgccgatt
      421 ccctcgagta ttacggtcgc cggaccggga agcacttcgg gaagctcgtg tatgccgccg
      481 gcaggctcct catgtcggcg cgggcacgcc gcatgaagct caccatcgat ggcgaacagg
      541 ttgtcgaacg tgccgtcatg tgcgaagtgg ccaatacgta ccggatggga ctgggtgcac
      601 ccgtggctcc cgaagccaac cccgccgacg gatggttgga tgtcgtcctg atcggcgacc
      661 tgtcgcgatg ggaactcctc cggtattacc gtgccgtgat ggcggagcgg cacctggagc
      721 ttcccaaggt gaaggcaacc cgggcccgcc gggtgaccat cgaggcactc caccccatga
      781 acgtgcactg cgacgaccgg atcatcggca ccactccggc ggaattctcg gtggcccggg
      841 cggcgctccg ggtcagggtg ccccggtcgt gaggcgctgg ctcgtcatgc cgctgggata
      901 cctgagttcg gcccgccagt cggtggcctt ctgggtgatg ctggccatgt tcctcggttt
      961 cctcacgctt tccctgctgg tccggtcgcc cggtctcacg cagcgcgatg cgatgctcac
     1021 cagggcaatc cagcactggc ggacacccgg gctcgactct ttcttccggg cggtcacctt
     1081 cctcggggat ccgattccac ccctcctggt ggcggcgttg gcttccgcat ggctcttccg
     1141 catggggcag caatggcccg gcgtgttcgt cctgatttcg accgcggggc tgccgctcaa
     1201 cttcctgatc aaggaattga ttcgcaggcc gcgaccggcc ggcgactcgg tcaacgtgct
     1261 cctcgacgtc gtcggccgga gctttccctc gggccatgcc atgacggcca ccatggtgta
     1321 cggcttcctg gcctatcttg gcttcgtgct tcccgccagg cctctgcatc gtgccctgct
     1381 cgtggccctc ttcacgatcc tcccactcct catcgggttc agccgcgtct acctcggggt
     1441 acactgggcc tcggacatcc tgggaggatg gacggccggc ctcttcttcc tgttcctcat
     1501 ggtgctggcc taccggctcg tcactgcggt ctgacctacg actggttgta gtagcgcatc
     1561 gcctccggga tttccttctg gaggttggca atgcgggtcc tgtccgacgg gtgcgtgctg
     1621 aggaattccg gcggcgcgcc cgaggagttg gccgacatcc gctcccagaa tgagacggct
     1681 tcgttggggt tgtagccggc catcgccatg aagatgaggc ccaggcgatc cgcttccgac
     1741 tcctggagac ggctgaaggg gagaagcgca ccgacctggg tgccgacgcc gaacgcggtc
     1801 atccagatct gacgcgtctg ggccggcttc gacgacagcg cttccgaaag cgcgagtccg
     1861 ccgagctgcg tcagcagccc ctggctcatc cgttcgttcc cgtgctcggc cacggcatgg
     1921 gcgatctcgt gtcccatcac gaccgccagt ccggcttcgg tctgcgtgac gggcaggatc
     1981 cccgtgtaga ccaccacctt tccgccgggc atgcaccagg cgttggcggc cttgtcttcc
     2041 acgaggttga actcccacgc atagccgtcg agctgcgagg agaggtcatg ctgacggaag
     2101 taagtttcca ccgcctgctg gatccggctt ccgacgcggc gcaccagctg gacctgggtg
     2161 gtattcccgc tcaggcgatg ctccttgaga aacgtgtcgt actcgtcgtt gctcatcgaa
     2221 aggatggtgg acgacgggat caggttgagc tgctgccgtc cggttaccgg caccttggaa
     2281 cagccggtcg gaaggacggt ggagccgagc aggacggcaa gcgcaagtcc gttggtgagc
     2341 gtgcggcgaa gagtcatcgt tcctccgatc aaacgaagcg accgcaccgg agcacggagc
     2401 gcgtcagtat tcagcatcac ggccctgtcg tcgcagtcat gcatcaggtg tgcccggccc
     2461 caagtcattg aaaacagggg agttggtcaa aactgacgcg cgccggcgcg acagtcggga
     2521 ggaaatgacg cgaaacacgg gaatctcgaa tgggaaattc ttgcgttgtc cggagcggag
     2581 cactccgcac cgacggtgta gtgccttctc tgataattgg ttatgggatt gctgacgaat
     2641 cccggtacgc cacttgcaac atcccccgga gcacgatgac ctgaaggctt ccatcgggaa
     2701 aggaagactt gaagcttcca tcggggatga aggaggactg tcatgagaac gatcgacacg
     2761 gatcgacaga acgaacttca cgagctgcac gtggtccgtc ccgagaatca cggaacgcgc
     2821 aatgctctcc tcatcggtgg cgcgatcctg gttctggcaa tcggcggcgc gctggcgctg
     2881 cagggaaaca acaatcttcc cgagggggag atggccggca acgtgtcgtc gcccgctccc
     2941 gtcaccccgg gagcctccac ttcgggctcg atcgccacac cccgtccgcc ggccgtcgcg
     3001 acgactccga caccggcccg tccgcgtccg gccgcttcga acctcgtaac cattccgcag
     3061 ggaacgccgg tcacggtgga gctgcgcaat gagatcggca cgaagcactc gcgcgtcggt
     3121 gacaccttca ccgccgtcgt cgcccagagc gtctcggcca acggccgcac cgccattccg
     3181 gccggctcga cggtgaatgg gcgggtcatc ctgtccgaac agcccggcaa ggccagcggt
     3241 cgaggcaagc tgcagctcca gttcaacacg gtctcgatca acgggcgttc ctacgacctg
     3301 ggttcgcgca gcgccgtctt cgaaagcaag tcgggcaccg agaaggatgt ggccatcatc
     3361 ggcggcggcg cggctgccgg tggtgtggca ggcgcaatcc tcggcaagga tgccggctct
     3421 gcggcaaagg gtgcggccat cggcggcgcc gccggcacgg tggcttcgct ccttacccgc
     3481 ggtccgcagc tcacgctcga acctggaacg aacatccgct tcacgctgga ccaaccggtt
     3541 gcagttcgac cgccctcgaa caccaacagc tgatcctcgg cgtgaagcgg cctttacaag
     3601 cgggcccccg gtcacttgac cgggggcccg tccctgttgt cccggaaccc gactctattc
     3661 gtcctggaaa gcgatgtctt gtctcagccc ccggtccccc gcgaggcctc ccggaccgcc
     3721 gcagtcagcg gatcatcctc gtcggctccc tcgagggcct gccgcgcccg ggccagcgcg
     3781 gcgcgggcct cctggacttc tccccgcgat tccaggatcc gtgcgaacga ccagttggcc
     3841 gccgggtcgt tcgggttcct ggccagcact ttctccagtc gccggagcgc ttcgtccggg
     3901 cgcttcgagt caatcagcgc tccgatcgtc tcccgttcga tgcgctgtcg ggcctcccaa
     3961 tggtgtgcag ccggcagggc cctgtccagg agatcaaccg cttcccgggg cgaccccgcg
     4021 cccagcagga gtagcgcctc caattcggga aggttgtagt aattatccac ctccgtgagg
     4081 tcacaccccc tggcccgggc ctgctgctga agcgcgagca acatcgcatc gagatattgc
     4141 cgggttccgg cccggtcgcc gaccgccagg gccacgcggg ccagcagtgc ctggtcggcc
     4201 ggcttcaagg catatgcgga acagtggacc tcggccagcg aggccgcttc ggcaatacgg
     4261 ccggcacgca ggtgcagcgc aatctgggta cggctccact ggcgcccttc cggcgactca
     4321 cccgagatgt cgcgggcccg ctggatggcg aagagggagg cttcccatct acccagctcg
     4381 gcattcacca gcgccaggtg atagtgggcc cagctgaagt cgggcttgat ctcgcaggcc
     4441 gtctcgaact gcgcgagtgc cttccgatag cgtcctgtcc tcagataaaa ctcgccaagg
     4501 ctgtcgtgcg ggttggcctg ctgttcgtgg acgaacacat atctctcgaa tgcatcctcc
     4561 gcttctggcc aggatcccag ctcggaacgc gcgtaaccga ggaggttcca cacgtcggtg
     4621 cgggcgggat cgatctcctc cgcccgttgc agttcggaga gcgcctcatc ggggcgccct
     4681 tccgacaagt gctggttggc cgccaggatc aggacgtcgg cgtcatcatg gaatcgccgg
     4741 atcagctcgt cttccagagc gcgctcgtcg ctctcgcgtc cttcgagatc ggcccccagg
     4801 aggcggattt cgagccgttc ccggtccgtc acgcgcgcct cctcgatcgt ggcccgtcgg
     4861 taccagtcga gcgccccttc gtcgtcggca agatcgtggg caaggcgtcc cagtcgcacc
     4921 atggccatcg cgaggtgcgg atcctcctcg agagccatca tccaggcggt cctggcttcg
     4981 ttgtcgtaga acttccggat ggcctcgatt ccatcgtcat agtagcggcg cgcctggctg
     5041 gacgcggtcg tcacctggct cccgaacgtg gcgggaagtg cgccgaggtt tcgtcgtccc
     5101 cgctggatga gcccgctgaa cgggatgccc acgcgcgcgt ctcgaaatcc caggttgagc
     5161 gtgccgagaa tcggcgcgtc agtggcaccg gcaccaggga tgggaatgcc gatgttgtgg
     5221 accacggagg gcgccgttgc acggcactcg gtccaggtgg cggtggtcgg gcaccacgcg
     5281 ccgcgcacga tggcggtacg agccgcttcc cggtccacat ccccgcgctg caccgacgcc
     5341 accgactggc cgtcggtgtc ctgaacgatc acccacgcca cgtcgggtcg ggccatcgca
     5401 tcctcgatga tgctcgccac ggtgcgcctc tcaccggcgg ccacgtgagg cgccagctgc
     5461 cgggccaggg ccgaggccac cgccatctgg gccgaccggc ctgtcggatc cggtgtccca
     5521 ccacacgaac cggcaacgag tgccagcacg agcacgagga gggtggagtg cctggatgtc
     5581 gagcgtgctt gtggagtggg gcgcatgccg cgagggggtc ccggggtgag tgaggcggcc
     5641 ggggaccgcg catcggtcgc cccggcaggc agggttatcg actggaccgc ggctcaactg
     5701 gaggagcggc ttgagtccgc tttggtgcgg cagtctggcc cggcctcgac ccgggccgct
     5761 gctggttccg aagatgttcg agcccatgaa attacgccgt ttcgggcctg actccgatcg
     5821 ccgcccggtt cgattctgac gcgagccggc tacgatagga tacgcgtgcc ttgagctggg
     5881 ccccgaggcc catgcagcgt ttcgagatcc gccgtcgtca cagggaatgt ccgtcgcttc
     5941 gaccccaagg gacccgatga acgaccagtc actgatatca cgaatactgg ccggcgatgt
     6001 cggtgcggag cgacagctgt acgacgcgca tgtcgatcgg atttaccggt tggcatgccg
     6061 gatgaccggc gatccgcttc acgcggaaga gttgacccag gagtcgttca tccgggcatt
     6121 cagccggttg gccacctttc gcggcgatgc ggccttcagc acctggatgc accaggtggc
     6181 cctctccgtg atcatctcgg ggctccgcaa gaagaagcgg atccgcgagc gggagacgag
     6241 tctcgagacc atgacgaact tcgatccggg ccgaccggcc gagcggcacg aactcaagag
     6301 gaaattgaag acggcggtcg acgatctctc cgagatcctg aagaccgtcc tcgtgatgta
     6361 cgaggtcgag ggttactcgc acggcgagat cgcctcgatc ctggaaatac cggaagggac
     6421 cagccgcgca cgactggccc gcgcgaagga gatcctccgc ggggtgctgg aacctccgaa
     6481 gatcgttggc ctggaaggag aattgcgatg agcgaccggc agttcgacga tctcctcaag
     6541 gagactctcg aggaatacgg cgagcccggc gccaccccgc gtgaagcgat gtggcgatcg
     6601 gtttcggagt cgcgtcgcaa ggtggtgagc ctgccgtggt ggcggcgtcc catgacgcgc
     6661 gtcgacttcc tggccggcgc cgtggcggcg gcggtcctca tcctggtggg gatcctcgtc
     6721 ggccggatga cgcagaaccc gccgaccccc acgggtcgga cgactccctc gttcaccgcc
     6781 gaatcgaatc cggccccgtc ggccccggtt gaatcgatcg atcgccccgt tccggacggg
     6841 atcgatcggg gaaccgccgt caatacgcga aagccggtga ggcacgacgt ggtccggacc
     6901 ccgtcgccct ctctcggtga gctcgccaag gttcccggcc aggcggaagg gagcgatgcc
     6961 cccctgccga gcgtggcgag cggcgagctg gagatcggga tgcggtccca gcccggtgac
     7021 gccgagcgcg aggttctccg actcgcggcc acgcgttacc tgtcgcgcac cgagtcgttc
     7081 ctcacc
//
LOCUS       DASVOD010000143         5354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_10195, whole genome shotgun sequence.
ACCESSION   DASVOD010000143 DASVOD010000000
VERSION     DASVOD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10195"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1726)
                     /locus_tag="VF720_02905"
     CDS             complement(62..1726)
                     /locus_tag="VF720_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878330.1"
                     /translation="MSAKSGRSGASDKPSGKSRPKSPGSGSGSGSGRSRRSGQLDRFA
                     GSPWALIPTFLLALAIRFIHLDQMNSYEDFRTPILDEAFSWDLASRFLAGFGSVGRPF
                     DADPGVALFVSVLVRVVGTRPDLVLACLAVLGSGAAVLVQLLGNRVLGGAAGLFCGLI
                     AAVYRPAIHHGAHVVDAAFVPFVAALALLLMAIQSESKRPLPELISGFLGGAATMLLV
                     LFQAPLIILAPIVLVVLIFDRKMKIPQKLRRGLSFSAGALGVMALLTVLNSPAAHGAG
                     SSPFLFRNVVPPGFGREFHLANRDGNRAGDRLPPHWAPADPAGLAAAYVEEGARVSAL
                     EGHVVPLTENARNPASVNAVAIDRAWSDRTGREAAGHPWGFLSGWAWRAWRNLNGAEA
                     PGPYDMAWESGRSWILRLPFPGWALLLPLALAGVMAARGASKDPALTLVVVWGVAVFI
                     AIWPFAVTSRARMAIEPALIVLAVRGAMRLFGLVGKRDMPALARAGLVVGIAAILVRL
                     PVGGTGPAPFHRLMGQAYESRGAIRAASEHRDEAERRSRGDTRLGR"
     gene            complement(1874..4486)
                     /gene="leuS"
                     /locus_tag="VF720_02910"
     CDS             complement(1874..4486)
                     /gene="leuS"
                     /locus_tag="VF720_02910"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEX7878331.1"
                     /translation="MTDDRTTTTTPDAGAKSPDEGGRAPREGSFPFAEYEEKWRAEWE
                     RQGLFRVDLASSKPKYYALNMFPYPSGDLHVGHGRNYIIGDVVVRRKLMDGHAVLAPM
                     GWDAFGLPAENAAIQNGIHPGVWTYNNIKRFKEQFAQWGIGLDWTREIAACHPGYYKW
                     TQWLFLTLYKKGLAYKAEAPVNWCPSCGTVLANEQVEDGACFRCGTTIEQRELSQWFF
                     RTTAYAQRLLDDLAQLDEWPDRVRTMQRNWIGRSEGVEIDFKRTDGPEVVRCFTTRAD
                     TLFGVTYVVIAPEHPLATALIGNDGTPLTADRKSRLQAMKNQRAERFYNPDLEKDGFD
                     TGKTAINPMNGEAVPVWVANYVLMNYGTGAVMAVPGHDARDYEFATKFGLPVRIVIEP
                     KLAADGGVAPVPEPGAAFEDDGVLVHSGDMTGKTSAEARGAIAAALEAKGSGKATITY
                     RLRDWLISRQRYWGAPIPIIYCPEHGAVPVPESQLPVLLPDDAEFKPTGESPLARHAA
                     FVNTTCPTCGKPARRETDTMDTFVDSSWYFLRYISPRDEARAFDTPMVNKWLPVDQYI
                     GGVEHAILHLMYARFITKALKDEGLLNFDEPFQRLFTQGMITKKSPLTGRLEKMSKSK
                     GNVVAPIDLINRYGADTVRLYTLFIGPPEKDAEWEDRAVEGAYRFLGRVWRLVGDMAD
                     RMPSSAGTIDQAGLSGATAELRFKVHDTIRQVGHDLTSFHFNTAVSGLMELVNAMSLY
                     QQSAEGARIAADTLEGRVWREACESLLLLLAPMAPHIAEELWRRTGHADSIFRMASPT
                     VDPAALVRTSVRIVIQVAGKLRGHLDLPADASPESIEAAALADPKIAPYLGDKPPMKV
                     IQVPGKLINIVPGR"
     gene            complement(4500..>5354)
                     /locus_tag="VF720_02915"
     CDS             complement(4500..>5354)
                     /locus_tag="VF720_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015196786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7878332.1"
                     /translation="NNTVWRETKVDFNPDDYEVHREFVTSTDGARVPLFVAHKRGLKR
                     DGRNPTLLYVYGGFNQPQVPSFTVSRVAWMEMGGVFALVCARGGGEYGREWHQAAVKL
                     TKQKTFDDVIAAGEWLQSNRYTTPAHLGLQGGSNGGMVVGAVTNQRPDLFGAVLAQAG
                     VMDMLRYDKFTIGKAWSADYGLSENEDEFKALYAYSPYHNVRPGTHYPAILVTTSDFD
                     DRVVPGHSFKYAAALQAAQAGSNPVLIRISRSTGHNGGMKPTMLAIDESADLWAFLAA
                     NLGMKPRL"
BASE COUNT          905 a         1789 c         1789 g          871 t
ORIGIN      
        1 tattgaaatc agccaactgt tgctgacaac agttgcccgc cgtccggaac gcccgacgac
       61 ctcagcgtcc cagccgcgtg tcaccccggg atctccgctc cgcctcgtca cggtgttcgg
      121 aagcggcgcg aatcgcgccg cgtgactcgt acgcctgccc catcaggcga tggaacggag
      181 ccgggccggt gccgccgacc gggagtcgca ccagtatcgc cgcgatccca acgaccaatc
      241 cggcccgcgc caacgcgggc atgtctcgct tgccgaccag gccgaaaagc cgcatcgcac
      301 cccgcaccgc caggacgatc aaggccggct cgatcgccat gcgcgcccgc gaggtgacgg
      361 cgaagggcca gatggcaatg aacaccgcga caccccacac cacgacgagg gtcagcgccg
      421 gatccttcga cgcaccgcgc gcggccatca cgcccgcgag ggccaatggc aagaggaggg
      481 cccagccggg gaaggggagg cggaggatcc atgaccgccc cgactcccag gccatgtcgt
      541 agggcccggg cgcctcggcg ccgttgaggt tgcgccacgc gcgccaggcc catcccgaca
      601 ggaatcccca gggatgtccg gccgcttcgc ggccggtccg atcggaccag gcgcgatcga
      661 tggcaacggc attgacgctg gccggattcc gggcattctc cgtgagcggc acgacatgtc
      721 cttccagagc cgacacgcgg gcaccttcct cgacatacgc cgccgcgagg cccgccgggt
      781 cggccggcgc ccagtgcggc ggcagtcgat cgccggcccg gttgccgtcg cgattcgcca
      841 ggtggaattc acgaccgaat cccggcggca cgacattccg gaacaggaat ggcgaagaac
      901 cggccccgtg ggctgcgggc gaattcagga ctgtcaggag cgccatcacg cccagggcgc
      961 cggcgctaaa tgacaatcca cggcgaagtt tctggggaat cttcatcttc cggtcgaaga
     1021 tgagaacgac caacacgatt ggcgccaata tgatgagtgg cgcctggaac agcaccagca
     1081 gcatcgtggc cgcgccgcca aggaaccctg aaatcaattc aggcagcggt cgtttcgatt
     1141 cggactgaat tgccatcagg agcagcgcca gggccgcgac gaacggcacg aacgccgcat
     1201 ccacgacatg cgcgccgtga tgaatcgccg gtcgatagac cgcggcgatc agtccgcaga
     1261 acagtcccgc ggcgccgccg aggacccgat ttccaagcaa ctggaccagg accgcggcgc
     1321 ccgagccgag cacggccagg catgccaata ccaggtccgg tcgcgtaccg accacgcgca
     1381 ccaggaccga cacgaacagc gccacacccg gatccgcgtc aaagggccgg cccacggagc
     1441 cgaatccggc caggaatctg gatgcgaggt cccatgagaa ggcctcgtcg aggatcggcg
     1501 tccggaagtc ctcgtacgaa ttcatctggt cgaggtggat gaatcggatc gcgagggcca
     1561 ggagaaaagt cgggatcagg gcccagggcg aaccggcgaa tcgatccagc tgaccggatc
     1621 gacgggagcg gcccgagccg gagccggagc ccgaaccggg ggactttggc ctggacttgc
     1681 cggagggctt gtccgaagcc ccggaacggc cggatttagc cgacatcagg ccccgcaagc
     1741 cccggcaatg tttggcgcat aatatatatt atgtaaacta acagataggg ggccggacgc
     1801 ctgccaggct ggcacatggc tcccgggtgc tctcgtaccg tggccccgcc cggccgacgc
     1861 caaccgatct cccctagcgc ccgggtacga tgttgatcag cttgcccggc acctggatga
     1921 ccttcatcgg tggtttgtcg ccgaggtacg gcgcgatctt gggatcggcc agggcggcgg
     1981 cctcgatcga ctccgggctg gcatccgccg gcaggtcgag atgaccgcgc agcttgccgg
     2041 ccacctggat gacgatccgc acgctggtgc gcaccagggc cgccggatcg acggtggggc
     2101 tggccatccg gaagatgctg tcggcgtggc cggtccgccg ccacagttcc tcggcgatgt
     2161 ggggcgccat gggcgccagc aacaacagga gcgattcgca ggcttcgcgc cagacgcgcc
     2221 cttccagggt atcggccgcg atccgagcgc cctcagccga ttgctgatac aggctcatcg
     2281 cattgaccag ctccatcagg cccgaaacgg cggtgttgaa gtgaaaagaa gtcaggtcgt
     2341 gaccgacctg gcgaatggtg tcatgcacct tgaagcgcag ctccgccgtg gccccggaga
     2401 ggccagcctg gtcgatcgtg ccggcggaag acggcatccg atccgccatg tcgccgacga
     2461 gccgccagac gcgtcccagg aagcgatagg ctccctcgac cgcgcggtct tcccattcgg
     2521 catccttctc cggcgggccg atgaagagcg tgtacaggcg gaccgtgtcg gcgccgtagc
     2581 ggttgatcag gtcgatcggg gccaccacgt tccccttcga cttcgacatc ttttccagcc
     2641 ggccggtcag cgggctcttc ttggtgatca tcccctgcgt gaagaggcgc tggaacggct
     2701 cgtcgaagtt cagcaggcct tcgtccttga gcgccttggt gatgaagcgc gcgtacatga
     2761 ggtgcaggat ggcgtgctcg acgccgccga tgtactggtc gaccgggagc cacttgttga
     2821 ccatcggcgt gtcgaaggcc ctggcctcgt cgcgcggtga gatgtagcgc aggaagtacc
     2881 acgacgaatc gacgaacgtg tccatcgtgt cggtttcgcg tcgcgccggt ttcccgcagg
     2941 tgggacaggt ggtgttcacg aacgccgcgt ggcgggccag cgggctctca ccggtcggct
     3001 tgaactcggc gtcgtccggc aggagcaccg gcagctgcga ctcgggcacc ggcacggcgc
     3061 cgtgctccgg gcagtagatg atcgggatcg gcgcgcccca gtagcgctgg cgggagatca
     3121 gccagtcgcg aaggcggtac gtgatcgtgg ccttgccgct ccccttggct tcgagcgcgg
     3181 ccgcgatggc gccgcgggcc tcggcactcg tcttccccgt catgtcgccg gaatggacca
     3241 ggacaccatc gtcctcgaac gcggcaccgg gctcggggac gggcgcgact cccccgtcgg
     3301 ccgccaactt cggttcgatc acgatccgga ccggcaggcc gaacttcgtg gcgaactcgt
     3361 agtcgcgcgc gtcgtgtccg ggcacggcca tgacggcccc ggtgccgtag ttcatcaaca
     3421 cgtagttggc cacccacacc ggcacggcct cgccgttcat cgggttgatc gccgtcttcc
     3481 cggtgtcgaa cccgtccttc tcgagatcgg gattgtagaa gcgctcggcg cgctggttct
     3541 tcatggcctg caggcgggac ttgcgatccg cggtcagcgg cgtgccgtcg ttgccgatca
     3601 gtgccgtggc cagaggatgc tccggcgcga tcacgacata agtgacgccg aacagcgtgt
     3661 cggcccgcgt cgtgaagcag cggacgactt ccggaccgtc ggtgcgcttg aagtcgatct
     3721 ccacgccttc gctgcgtccg atccagttcc gctgcatggt gcggacgcgg tccggccatt
     3781 catccagctg ggccaggtcg tccagcaggc gctgtgcgta ggccgtggtc cggaagaacc
     3841 actgcgacag ctcacgctgc tcgatggtgg tgccgcagcg gaaacaggcg ccatcctcca
     3901 cttgctcgtt ggccagcacc gtgccgcacg acggacacca gttgaccggc gcctcggcct
     3961 tgtaggccag ccccttcttg tagagcgtga ggaagagcca ctgcgtccac ttgtagtagc
     4021 cggggtggca ggcggcgatt tcgcgcgtcc agtcgagccc gatcccccac tgggcgaact
     4081 gctccttgaa gcgcttgatg ttgttgtagg tccagacgcc ggggtgaatg ccgttctgga
     4141 tggccgcatt ctcggccggg aggccgaagg cgtcccatcc catcggcgcg aggacggcgt
     4201 ggccatccat gagcttccgg cgcacgacga cgtcgccgat gatgtagttg cggccgtgcc
     4261 ccacgtggag atctcccgag gggtacggaa acatgttcag ggcgtagtac ttcggcttcg
     4321 aggaggcgag gtcgacccgg aacagcccct ggcgctccca ctcggcgcgc cacttctcct
     4381 cgtactcggc gaacggaaac gacccttcgc gcggcgcgcg acccccctcg tcgggcgact
     4441 tcgctccggc gtcgggtgtc gtggtggtcg tgcgatcgtc ggtcatgatc tcctctttcc
     4501 tacagacggg gcttcatccc caggttggcg gccaggaagg cccacaggtc ggctgattcg
     4561 tcgatggcca gcatcgtggg cttcatgccg ccattgtgcc cggtcgagcg gctgattcgg
     4621 atcagcaccg gattgcttcc cgcctgggcg gcctgcaggg cggcggcata cttgaagctg
     4681 tgaccgggca ccacgcggtc atcgaaatcg gacgtggtga ccaggatggc cgggtagtgt
     4741 gttcccggtc ggacgttgtg gtacggcgag taggcgtaca gcgccttgaa ttcgtcctcg
     4801 ttctcggaga ggccgtagtc ggccgaccag gcctttccga tcgtgaactt gtcgtagcgg
     4861 agcatgtcca tcacgccggc ctgggccagc acggcgccga acaggtcggg ccgctggttg
     4921 gtgaccgcgc cgacgaccat cccgccgttc gatcccccct gcagacccag gtgggccggc
     4981 gttgtgtagc ggttggactg gagccactcc cccgccgcga tgacgtcgtc gaaggtcttc
     5041 tgcttggtca gtttcaccgc ggcctggtgc cactcgcggc cgtactcgcc gccgccgcgg
     5101 gcgcagacga gggcgaacac gccccccatc tccatccagg ccacgcggga gacggtgaac
     5161 gacggcacct gcggctggtt gaagccgccg taaacataga gaagcgtggg attccggccg
     5221 tcgcgcttca gcccccgctt gtgagccacg aagagcggca cccgcgcgcc gtcggtggag
     5281 gtgacgaact cccggtgcac ctcgtaatcg tccgggttga aatcgacctt cgtttcccgc
     5341 cagacggtgt tgtt
//
LOCUS       DASVOD010000144         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_896262, whole genome shotgun sequence.
ACCESSION   DASVOD010000144 DASVOD010000000
VERSION     DASVOD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896262"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2095
                     /locus_tag="VF720_02920"
     CDS             <1..2095
                     /locus_tag="VF720_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878333.1"
                     /translation="SFIAKWNGQEWFPLAEGFDDYVGALEVYGEQLVAGGRFIFSGAE
                     YIPRVAAWDGAAWTRIDSGIYYDRVDALLVWNKKLIAAGHFESAGSVLTRNIAQYDGR
                     KWQPLGAGLGGAIELVTSLVPWHGELVAGGLFNPAGSTFAPGIASWNGARWKPLLTTF
                     GAPENTVPGIYALASSSTHLYAAGWFNLINGASVKGIAGTDGTSWVPLGAGAEKAFFE
                     LAVDPASGRVLAGGAFETIGGIRASGVAEYDGAWHPLGRGEPVDDAVRALGTWDGHVV
                     AAGDFVRSGLIESNGVALWKGGDWQPLGDGFDDRVEALTEWQGTIVAGGYFGASGATP
                     LSRIAIWRDETWQDLGGGVDGAVWALVPYGPDLVAAGEFLTAGGVTVNRVARWDGSAW
                     HPFGAGMNAPVRDLTTWNGQLVAGGFFTQADNAPSNRVARWDGTAWQPLGAGLNGRVT
                     AVAAWNGRLVAAGNFLSAGNVFANRIAAWDGSLWAPLGVGFPVSGSAIGKLVSWNGAL
                     LAGGSFTEVGNVFANQVAAWNGTDWSAIGQGIRKIGIAGDVPTRIDEFLPTPDGLYVS
                     GDFNRAGGKSSVNMAILAPVAAPAQPLAGVDADHPSPDAHSLRLLAPAITTRSGVARL
                     AYERAHAGPVRIDIVDVLGHPVARLTGQDPAGRSERNWDGLDWAGRATSAGVYFARVS
                     DGVTSAVVRIVRLDR"
     gene            complement(2143..2853)
                     /locus_tag="VF720_02925"
     CDS             complement(2143..2853)
                     /locus_tag="VF720_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX7878334.1"
                     /translation="MSDVTLTWLGHGSLLVGAADGRQILIDPWVGQNPACPDTLKKSV
                     RPDVMLVTHGHGDHIGDAVSLARAHEPDIIGIVELCHWLESKGVGKTRGMNKGGTQAL
                     GDMRVTMVHADHSCGILDDGKMVYAGEAVGFVLRFPDDLSVYVAGDTNVFGDMKLIAD
                     LYRPDIAVLPIGDHYTMGPAEAALAARLLGVNRVVPTHWGTFPILTGTPEKLSAALGG
                     VGPEIVALRPGESTKLGR"
     gene            complement(2898..>3603)
                     /locus_tag="VF720_02930"
     CDS             complement(2898..>3603)
                     /locus_tag="VF720_02930"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX7878335.1"
                     /translation="AKGLAWARVTDAGLEGGSAKFLDAPKVIAALSATPGDLVLFVAD
                     TKRDVACKSLGAVRTVLGEPLVAGRQNEFNFLWVREFPLFEQADDGSWVPAHHIFSMP
                     LEEHLDALESDPGRVHAQLYDLVCNGVEMASGSIRIHRRDIQERVMKVIGLSIEEAEN
                     RFGFLLEAFQYGAPPHGGIAPGLDRLIMVMTGRKSLRDVIAFPKTQSATSLMDGCPAE
                     VDARDVKDLHLKIVTE"
BASE COUNT          535 a         1240 c         1259 g          569 t
ORIGIN      
        1 ctccttcatc gcgaaatgga acggccagga gtggtttccc ctggcggaag gattcgacga
       61 ctacgtcggg gcgctcgagg tgtacggcga acaactcgtg gcggggggcc ggttcatctt
      121 cagcggtgcg gagtacatcc ccagggtggc cgcctgggac ggtgccgcct ggacgcgcat
      181 cgactcgggg atctattacg accgggtgga tgcgcttctc gtctggaaca agaagttgat
      241 cgcagccggc cacttcgagt cggccggcag cgtcctgacc cgcaacatcg cccagtacga
      301 cggcaggaag tggcagccgc tgggcgccgg cctcggcggg gccatcgaac tcgtcacctc
      361 gctcgtgcca tggcacgggg aactcgtggc cggcggactc ttcaacccgg ccggctccac
      421 cttcgcgccc ggaatcgcct cgtggaacgg cgcgcgatgg aagccccttc tcaccacctt
      481 cggggcgccc gagaacacgg tgcccggcat ctacgcgctg gcctcctcgt ccacgcatct
      541 ctacgccgcc ggctggttca acctcatcaa tggcgcctcc gtgaagggaa tcgcggggac
      601 ggacggcacc tcgtgggtcc cgctcggcgc cggggcggag aaggccttct tcgagctggc
      661 cgtcgatccc gcttccgggc gtgtgctggc ggggggcgcc ttcgaaacga tcggcggcat
      721 ccgggccagc ggcgtggcgg aatacgacgg cgcctggcat ccgctgggga ggggcgaacc
      781 ggtcgacgac gccgtgcgcg ccctcggcac ctgggatggc cacgtagtgg ccgccgggga
      841 tttcgtccgg agcggcctca tcgaatcgaa cggcgtggcc ctctggaagg gtggcgactg
      901 gcagccgctg ggcgatggat tcgacgaccg ggtcgaggcg ctcaccgagt ggcagggaac
      961 gatcgtggcc ggcggctatt tcggcgcctc gggcgcaacg ccactctccc gcatcgccat
     1021 ctggcgcgac gagacctggc aggacctggg cggcggcgtc gatggcgcgg tgtgggcgct
     1081 ggtcccctac gggcccgacc tggtcgccgc cggagaattc ctcacggcag gtggcgtcac
     1141 ggtaaaccgc gtggcccgat gggacggaag cgcctggcat ccattcggcg ccggcatgaa
     1201 cgcgccggtg cgcgacctca cgacgtggaa cggccagctg gtggccggcg gcttcttcac
     1261 ccaggccgac aacgcaccgt ccaatcgtgt cgcgcgctgg gacggcactg cctggcaacc
     1321 gcttggcgcc ggcctcaacg gtcgcgtgac ggcggtggcc gcctggaacg gccggctggt
     1381 ggcggccggc aacttcctct cggccggcaa cgtgttcgcc aatcgcatcg ccgcgtggga
     1441 cggaagcctg tgggcaccgc tcggcgtcgg ttttccggtg agcggcagcg cgatcgggaa
     1501 actcgtctcc tggaacgggg ccctgctcgc ggggggctcg ttcaccgagg tggggaacgt
     1561 tttcgccaac caggtggccg catggaatgg aacggactgg agcgcgatcg gccagggaat
     1621 ccggaagatc gggatcgccg gcgacgtgcc gacgcgcatc gacgaattcc tgccgacacc
     1681 cgacggcctc tacgtgtcgg gcgatttcaa ccgggccggc ggcaagtcgt cggtgaacat
     1741 ggcaatcctc gcgccggttg ccgcacccgc ccagccgtta gccggcgtgg acgccgacca
     1801 cccatccccc gacgcgcact cgctccggct gctcgctccg gcgatcacca cgcgctcggg
     1861 cgtggcacgc ctggcctacg aacgtgcgca cgccggcccg gtccgcatcg acatcgtgga
     1921 tgttctcggc cacccggtgg cgcgcctgac cggccaggac ccggccggac ggagcgaacg
     1981 aaactgggac gggctcgact gggccggacg cgcaacgtcg gctggcgtct acttcgcccg
     2041 cgtgtcggac ggcgtgacgt cggcggtcgt ccggatcgtg cggctcgatc gataggagaa
     2101 cccggcgatc caccgccgat ccccccggaa cgccggttga cgctatcgtc cgagcttcgt
     2161 cgactccccc ggcctgagcg ccacgatctc cggccccacc ccaccgagcg ccgccgagag
     2221 tttttccggc gtgcccgtca ggatcggaaa cgtgccccaa tgcgtcggga cgacgcggtt
     2281 cacgcccagc aggcgcgcgg cgagcgccgc ctcggccggg cccatcgtgt agtggtcccc
     2341 gatcggcagc accgcgatgt ccggccggta gaggtcggcg atgagcttca tgtcgccgaa
     2401 gacgttcgtg tctcccgcca cgtagaccga caggtcgtcc gggaaccgga ggacgaagcc
     2461 gacggcctcc cccgcgtaca ccatcttccc gtcgtcgagg atgccgcacg agtggtcggc
     2521 gtggaccatc gtcacccgca tgtcgccgag cgcctgcgtc ccacccttgt tcatgccgcg
     2581 cgtcttcccg acccccttcg actcgagcca gtggcagagt tccacgatgc cgatgatgtc
     2641 gggctcgtgg gccctggcca ggcttacggc gtcgccgatg tggtcgccgt ggccgtgcgt
     2701 cacgagcatg acgtcgggcc tcacgctctt cttgagggta tcggggcagg cggggttctg
     2761 cccgacccat gggtcgatca ggatctggcg gccgtcggcg gctccgacga gcagcgagcc
     2821 gtggccgagc caggtgagtg tcacgtcgga catgtcgttc ctctcgttgt tccgccgggc
     2881 cgcatcggtc cgtcgggcta ttcggtcacg atcttcaagt gaaggtcctt cacgtcacgc
     2941 gcgtcgacct cggcggggca cccgtccatg aggctggtgg cgctctgcgt cttgggaaag
     3001 gcgatgacgt cgcgcagcga tttccggccg gtcatcacca tgatgaggcg gtcgaggcct
     3061 ggcgcgatgc cgccatgcgg cggcgccccg tactggaacg cctcgagcag gaagccgaag
     3121 cggttttcgg cctcttcgat ggagagcccg atcaccttca tcacccgctc ctggatgtcg
     3181 cggcggtgga tacggatcga acccgaggcc atctcgacgc cgttgcacac caggtcgtag
     3241 agctgggcat gcaccctgcc ggggtcggac tcgagcgcgt cgagatgctc ctcgagcggc
     3301 atcgagaaga tgtggtgcgc cggcacccac gagccgtcat cggcctgctc gaagagcggg
     3361 aattcccgca cccagagaaa gttgaactcg ttctgccggc cggccaccag cggctcgccg
     3421 agcacggtgc ggacggcacc caggctcttg cacgccacgt cgcgcttcgt gtcggccacg
     3481 aagaggacga gatcgccggg agtggcggaa agtgcggcga tcaccttcgg cgcgtcgagg
     3541 aacttggccg agcccccctc gaggccggca tcggtcaccc tggcccaggc gagccccttc
     3601 gca
//
LOCUS       DASVOD010000145         1540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_61867, whole genome shotgun sequence.
ACCESSION   DASVOD010000145 DASVOD010000000
VERSION     DASVOD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61867"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VF720_02935"
     CDS             <1..557
                     /locus_tag="VF720_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878336.1"
                     /translation="DRLWTEQRELATLGTRRLTLEDVSDVVSDVESSFSEEINKKKAR
                     VIRRLTNGLPPLLLDVAQVRRVLTEIVSGLIETLPIGGRLKLETRRGGDQVQVLLGAD
                     GPRRPGDSLDRLFLAFTPAARPADGPITGLVRQVIREHGGEIGVRADVDWPALYVLSF
                     PIRTNQDRRRPATDRRIGRDRRAA"
     gene            650..725
                     /locus_tag="VF720_02940"
     tRNA            650..725
                     /locus_tag="VF720_02940"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:683..685,aa:Phe,seq:gaa)
     gene            778..853
                     /locus_tag="VF720_02945"
     tRNA            778..853
                     /locus_tag="VF720_02945"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:811..813,aa:Thr,seq:cgt)
     gene            917..>1540
                     /locus_tag="VF720_02950"
     CDS             917..>1540
                     /locus_tag="VF720_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX7878337.1"
                     /translation="MSQLMISVSGIRGIVGEALTPEVVAHYAARLATWLPAGPIVFGR
                     DSRPSGPVLRHAILAAMTGVGREVIDLGVVPTPTVQVEVEQRRAAGGIALTASHNPVE
                     WNALKLIGGDGRFLTATTGRAFLDAAATMTPAWKSWDGLGHVTTAVDPLSRHLGLILG
                     LDFLPVADIRARGFRVALDAVHGAGGLIGRQLLEALGCRAEILFAEPT"
BASE COUNT          227 a          534 c          524 g          255 t
ORIGIN      
        1 tcgaccggct ctggacggaa cagcgcgagc tggccacgct cggcacgcga cgcctcacgc
       61 tggaagacgt gagcgacgtc gtcagcgacg tcgagtcctc gttcagcgaa gagatcaaca
      121 aaaagaaggc gcgggtgatc cggcgcctga ccaacggcct gccgcccctg ctcctcgacg
      181 tggcgcaggt gcgacgcgtc cttaccgaga tcgtgtcggg cctgatcgag acgcttccga
      241 taggggggcg cctcaagctc gaaacgcggc gcggcggcga ccaggtgcag gtgctgctcg
      301 gtgctgatgg tccgcgtcgt ccgggagatt cgcttgaccg gctcttcctg gctttcacgc
      361 ccgcggcccg tccagccgac ggcccgatca cggggctcgt ccggcaggtc atccgcgaac
      421 acggcgggga gatcggcgtc cgggccgatg tcgactggcc ggccctctac gtcctgagct
      481 ttccgatccg gaccaatcag gatcggcgcc gcccggcgac cgaccgccgg atcgggcgcg
      541 atcgccgggc ggcctgatac ggcatcagcg ccgcggacaa gcagttccgc gttgacgatc
      601 ggcgccaccg gctggtaatc tcccggacgc ttttcaccac gacattggcg cgaccctagc
      661 tcagttggta gagcaccaga ctgaaaattt gggtgtccgc ggttcgattc cgcggggtcg
      721 caccaccttg gcgcaaggca taccgcggcc ggaccagtcg cggggttccg tccggacgct
      781 ggtgtagctc agcaggtaga gcaacgcact cgtaatgcgt aggtcagcgg ttcgattccg
      841 ctcaccagct ccattctccc gccgcggggc ccttcccgca caggtaatcc cgccgtgttc
      901 caattcaggt gatcccgtgt cgcaactgat gatcagcgtc tccggcatcc ggggaatcgt
      961 gggggaggcc ctcacgccgg aagtcgtggc gcattacgcc gcccgactgg ccacgtggct
     1021 gccggccggg cccatcgtct tcggtcgcga ctcgcgtcct tccggaccgg tccttcgcca
     1081 cgccattctc gcggccatga ccggtgtcgg gcgcgaggtc atcgacctcg gggtcgtgcc
     1141 cacgcccacg gtgcaggtgg aggtggagca gcgccgggcg gccggcggca tcgcgcttac
     1201 cgccagccac aacccggtgg agtggaacgc gctcaagctg atcggcggcg acggccggtt
     1261 cctgaccgcc accacggggc gtgcgtttct cgatgccgcc gccacgatga cgcccgcatg
     1321 gaaatcttgg gacggcctcg ggcacgtgac gacagccgtc gacccgctgt cgcggcacct
     1381 ggggctgatc ctcggcctcg acttcctgcc ggtggccgac atccgcgccc gcggtttccg
     1441 cgtggcgctc gatgcggttc acggtgcagg cggcctgatc ggccggcagc tgctcgaggc
     1501 actgggttgc cgggcggaga tccttttcgc ggagccgacc
//
LOCUS       DASVOD010000146         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_166681, whole genome shotgun sequence.
ACCESSION   DASVOD010000146 DASVOD010000000
VERSION     DASVOD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166681"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF720_02955"
     CDS             complement(<1..203)
                     /locus_tag="VF720_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX7878338.1"
                     /translation="MTDDMLGRGFSHYQVTRRLGSGGMGVVYEADDSRLGRKVALKFL
                     PAELQQDPHALERFQREARAASGL"
     gene            complement(420..>1893)
                     /gene="groL"
                     /locus_tag="VF720_02960"
     CDS             complement(420..>1893)
                     /gene="groL"
                     /locus_tag="VF720_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX7878339.1"
                     /translation="KEIDLEDVYENIGARMVREVASKTSDVAGDGTTTATVLAEAIFK
                     QGLKNVTAGANPMDLKRGIEKAVETVTAELKKLSKSVKGSTTEIEQVATISANGDSFI
                     GKIIADAFSKVGQEGTITVEEAKSMETTLEVVEGMQFDNGYLSPYFINDKDGMECNLE
                     NAYILLNEKKISNMKDLLPLLEQIAKAGRPLLIISEDVEGEALATLVVNKLRGTLNVC
                     AVKAPGFGDRRKAMLQDIAILTGGQVITEDLGIKLENITLKDLGQAKNISVTKDNSTI
                     IKGAGKEADIKARIGQIKKQIEETTSDYDREKLQERLAKLAGGVAVVNVGAATETEMK
                     EKKARVEDALHATRAAVEEGIVPGGGTALIRCVPAIKKMMETLEGDQKIGADIVCRAI
                     EEPLRTLVNNAGLEGSVVVEKVRNEKKAVGFNVDTEQYVDMFEAGIIDPTKVTRSALQ
                     NAASIAALMLTTEALVTEVPDKDNKPAGMPGGGGYGGDDF"
BASE COUNT          255 a          587 c          635 g          416 t
ORIGIN      
        1 agcccggaag cggcccgcgc ctcccgctgg aagcgctcga gcgcatgcgg atcctgctgc
       61 agttcggcgg gaaggaactt gagggccacc ttgcgcccca ggcgggaatc gtcggcctcg
      121 taaaccacgc ccattccacc acttccaagg cgtcgggtga cctggtagtg ggagaagccg
      181 cggccaagca tgtcatcggt catgggcgat gattatacgg ggcggtgcgg tgatcccgaa
      241 gcgggaagat tgtgcgggga ccgagccgtc aaggcgtcgg tgcgatcggg tgcgcctcaa
      301 cgccagaaac gggcgccgtc tccggcgccc gttccgctgc agttctcgtt gtcttgtcag
      361 ctaatcccgg cggccggccg gtggttctga tgtggccggc cgccggtcat caatctggtc
      421 tagaagtcgt ccccgccgta gccgccgccg cccggcatgc cggccggctt gttgtccttg
      481 tccggcactt cggtgacgag ggcttcggtc gtgagcatga gcgccgcgat ggaggcggcg
      541 ttctgcaggg ccgagcgggt gaccttggtc gggtcgatga tgccggcctc gaacatgtcc
      601 acgtactgct cggtgtcgac gttgaagccg acggccttct tctcgttgcg caccttctcg
      661 acgacgaccg agccttcgag gccggcgttg ttgaccaggg tgcggagcgg ctcctcgatg
      721 gcgcggcaga cgatgtcggc gccgatcttc tggtcccctt caagcgtctc catcatcttc
      781 ttgatggccg gcacgcagcg gatgagcgcg gtgccgccgc cggggacgat cccttcctcg
      841 accgccgcac gggtggcgtg gagggcgtcc tctacgcggg ccttcttctc cttcatctcg
      901 gtctcggtgg cggcgccgac gttgacgacg gccacgccgc cggccagctt ggcgaggcgc
      961 tcctggagct tctcgcggtc gtagtcggac gtcgtttcct cgatctgctt cttgatctgg
     1021 ccgatgcggg ccttgatgtc ggcttccttg ccggcgccct tgatgatcgt gctgttgtcc
     1081 ttggtgaccg agatgttctt ggcctggccg aggtccttca acgtgatgtt ttcgagcttg
     1141 atccccaggt cttcggtgat gacctggccg ccggtcagga tggcgatgtc ctggagcatg
     1201 gccttgcggc ggtcaccgaa gcccggcgcc ttcacggcgc agacgttcag cgtgccgcgg
     1261 agcttgttga ccaccagcgt ggccagcgcc tcgccctcga catcttcgga gatgatgagg
     1321 aggggccggc cggccttggc gatctgctcg agaagcggga gcaggtcctt catgttcgag
     1381 atcttcttct cgttcagaag gatgtaggcg ttctcgaggt tgcactccat cccgtccttg
     1441 tcgttgatga agtacgggga gaggtagccg ttgtcgaact gcattccctc gacaacttcg
     1501 agcgtcgtct ccatcgactt ggcttcctcg acggtgatcg tgccttcctg gcccaccttc
     1561 gagaacgcgt cggcgatgat cttgccgatg aacgaatcgc cgttggcgga gatggtggcc
     1621 acctgctcga tctcggtggt ggagcccttc acgctcttgg agagcttctt gagctcggcg
     1681 gtgaccgtct cgacggcctt ctcgatgccg cgcttcaggt ccatcgggtt ggcgccggcg
     1741 gtgacgttct tgaggccctg cttgaagatg gcttcggcaa ggaccgtggc ggtggtggtg
     1801 ccgtcgccgg ccacgtcgct ggtcttgctg gcgacttcgc gaaccatgcg agcgccgatg
     1861 ttttcataaa cgtcttccag gtcgatttcc ttg
//
LOCUS       DASVOD010000147         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_322396, whole genome shotgun sequence.
ACCESSION   DASVOD010000147 DASVOD010000000
VERSION     DASVOD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322396"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VF720_02965"
     CDS             complement(<1..1127)
                     /locus_tag="VF720_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEX7878340.1"
                     /translation="MTLPVLAVIVLAVLWLGYHFYGRLVARQFRLNAGTETPAVRHND
                     GNDFIPTKPFYLLSQHFAAIAAAGPIAGPILACQAFGWLPCVLWIGFGVIFIGAVHDL
                     SALVASVRHDARSVAEIVKENIGKRAWVAIMLFIWIALMYVIVAFADITANTFLGRIE
                     ELDGVAVGFERGGAVAAASTFYLLLSVVMGLVQKRFNPPLWLVTLIFVPATLGVVWLG
                     TRFDTLMVLPAIPHLPAGKAWGLLILAYCFVASLLPMWLLLQPRGYLGGFVLYLALAA
                     GVIGIFFGGFPIQQESFKTWNGPGVTGSLFPFLFVTIACGACSGFHGLVCSGTTSRQI
                     ANETHCQPVGYGAMLLEAFVALIALSTVMIVSQATISAGKLA"
     gene            complement(1189..1911)
                     /locus_tag="VF720_02970"
     CDS             complement(1189..1911)
                     /locus_tag="VF720_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878341.1"
                     /translation="MKISRLVLGGVLALAPSSAHAFGNANAKVQLHLTPPFTSQVCSR
                     AESNPSCDQIDTSGGLYPTNYFVHLLITDGDPVEGVVTLANSIQYDAAPGSGVDVWTW
                     SLCARLELASAGWPSSGGRNLISWGSGNCERNEPGGPGTGVVAHAGYFYISAYSADVM
                     RVVPNENNPGQPGLYRKVVVSNCDGQPDIIEDLDNPNVPSHLGSVAFSATGLVEGTNP
                     CLKIVPVTSATWSRIKSGLIDE"
     gene            complement(1908..2636)
                     /locus_tag="VF720_02975"
     CDS             complement(1908..2636)
                     /locus_tag="VF720_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878342.1"
                     /translation="MKLARIVPALLALSLLPNVAHAYGNANAKIVLHLAATTTVAPCT
                     RAAAHPSCEQIDTSGGLYPNLYYAYVLITDGSAAEGVAGAEFGIDYNGDYGVGVEIYL
                     WTLCATLELGGREPGWPNADSATLITWDASTRCQRNEPGGPGSGVVATAGYFYCAAYN
                     ADTLRIVPRRRLPSHTYARVRVANCASQEDIIDDRDAPASPSPLGSVAFSASGLEEGT
                     NPCQKIVPVQGTTWSRLKSGGNTE"
     gene            complement(2800..>3119)
                     /locus_tag="VF720_02980"
     CDS             complement(2800..>3119)
                     /locus_tag="VF720_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX7878343.1"
                     /translation="PAPVRFLPEFDNLMLSHHDRSRVLADAHRPKVVSKNLQVAATFL
                     VDGFVAGTWKVEAKKSVATLRLAPIGKIDKGTKAELEREGEALLAFLEPEAAGRSVTF
                     TSA"
BASE COUNT          584 a         1018 c         1029 g          488 t
ORIGIN      
        1 gccagcttgc cggccgagat cgtggcctga ctcacgatca tcaccgtgga gagcgcgatc
       61 agggccacga acgcctcgag gagcatcgcc ccgtatccca ccggctggca gtgcgtttca
      121 ttggcgatct ggcgcgaggt cgtgcccgag cagacgaggc catggaatcc cgaacaggcc
      181 ccgcacgcga tcgtcacgaa cagaaatgga aacaggctcc ccgtgacacc cggcccgttc
      241 cacgtcttga agctctcctg ctggatcgga aacccgccga agaagatgcc gatgacgccg
      301 gccgccagcg cgaggtacag gacgaatccg ccgagataac cgcgcggttg cagcagcagc
      361 cacatcggca acagcgaggc cacgaagcaa taggccagga tcaggagccc ccacgccttg
      421 cccgccggca ggtgcggaat ggccggcagc accatcaggg tatcgaaccg cgtgccgagc
      481 cagaccacgc ccagcgtggc cggaacgaag atcagcgtca ccagccagag cggtggattg
      541 aagcgcttct gcaccagccc catcaccacc gacagcaaca ggtagaaggt gctggccgcc
      601 gccaccgccc cgccccgctc gaatccaacg gccacgccat ccagttcctc gatccgcccg
      661 agaaaggtgt tggccgtgat gtcggcgaac gccacgatca cgtacatgag cgcgatccag
      721 atgaagagca tgatcgccac ccaggcccgc ttcccgatgt tctccttcac gatctccgcc
      781 accgaccgcg cgtcgtgccg caccgacgcc accagcgccg acaggtcgtg caccgccccg
      841 atgaagatca ccccgaaccc gatccagagg acgcacggca gccacccgaa cgcctggcag
      901 gccaggatcg gccccgcgat cggcccggcg gccgcgatgg ccgcaaagtg ctggctgagg
      961 agatagaagg gtttggtggg gatgaagtcg ttgccgtcgt tatgccggac ggcgggggtc
     1021 tcggtgccgg cgttcagtcg gaactgacgc gcgaccaggc ggccgtagaa gtgatagccg
     1081 agccagagga cggcgaggac gatgacggcc aggacgggga gcgtcatggc gcgtcatcct
     1141 agggatgcgg gcgggagtgt caaggcacac cccccgcccg caatcagact attcgtcgat
     1201 cagtcccgac ttgatgcggc tccaggtcgc actcgtgacc ggcacgatct tgaggcaggg
     1261 gttcgtcccc tcgacaagcc cggtggccga gaaggcaacc gaacccaggt ggctcggcac
     1321 attcgggttg tccagatcct cgatgatgtc gggctggccg tcacagttgc tcacaacgac
     1381 cttccggtag agtcccggct ggcccggatt gttttcgttg ggaacgacgc gcatcacgtc
     1441 cgcactgtac gcgctgatgt agaagtatcc ggcgtgggcc acgacgccgg tgccgggccc
     1501 ccccggctcg ttccgctcgc agttcccgga cccccaggaa atcaggtttc gaccgcccga
     1561 ggaaggccag cctgcggagg cgagctccag gcgggcacag agactccacg tccagacgtc
     1621 gacccccgaa cccggagccg catcgtactg gatgctgttg gcgagggtga cgacgccctc
     1681 gacagggtcg ccgtccgtga tcagcaggtg aacgaagtag ttggtgggat aaagaccccc
     1741 gctggtatcg atctgatcac atgacgggtt ggactcggcg cgcgagcaca cctgggatgt
     1801 gaatggcggc gtgaggtgca actgcacttt ggcatttgca ttgccgaagg cgtgggcgga
     1861 cgacggcgcc agcgccagga ccccgcccag gacgagcctg gagattttca ctcggtgttc
     1921 ccacccgact tgagtcgact ccacgtcgtt ccctggacag gcacgatttt ctgacaggga
     1981 ttggtccctt cttcgaggcc cgatgcggag aaggccaccg acccaagcgg gctgggtgac
     2041 gccggcgcgt cacgatcgtc gatgatgtct tcctgcgagg cgcagttggc cacgcggacg
     2101 cgggcgtacg tgtgcgacgg cagccgccgg cgcgggacga tccgcaaggt gtccgcattg
     2161 taggcggcgc agtagaagta gccggccgtc gccacgacgc ccgacccggg cccgcccggc
     2221 tcgttccgct ggcaccgggt cgaggcatcc caggtgatca gggtggcgga atcggcgttg
     2281 ggccagcccg gttcccgtcc cccgagttcg agtgtggcgc agagcgtcca taggtagatc
     2341 tcgaccccca cgccatagtc gccgttgtaa tcgatgccga actccgctcc ggccacccct
     2401 tcggcggcac tgccatccgt gatgagaacg taggcgtagt acagattggg atacaggccg
     2461 ccgctcgtgt cgatctgttc gcacgacgga tgggcggccg cccgggtgca gggcgccacc
     2521 gtcgtggtgg cggccaggtg gaggacaatc ttggcgttgg cgttgccgta ggcgtgggcc
     2581 acgttgggga ggagggacag ggcgagcagg gccggaacaa tacgagcgag tttcatggtt
     2641 gtctcccgtg ggcaggtacc agaagttgcc agtaacgctc gagcgggccg ggaggctcag
     2701 gtcgcggcat ccgaggaggg ttcagtcttc cagatggaga ggggcttgtc cagtcagaat
     2761 tgatatggat tcagtcccat atgaccgtca cggggccggt caggccgatg taaacgtcac
     2821 ggagcgaccg gccgcctcgg gctcgagaaa tgccagcagg gcctcgccct cccgctccag
     2881 ctcggccttg gtccccttgt cgatcttccc gattggcgcc aatcgaagcg tggccaccga
     2941 tttcttcgcc tccaccttcc acgtccccgc cacgaaccca tccaccagga aggtggccgc
     3001 cacctgtaaa ttcttcgaca caacctttgg acgatgcgcg tccgccagga cgcgcgaccg
     3061 atcgtgatgc gacagcatca ggttgtcgaa ctcggggagg aagcgcaccg gcgccgggg
//
LOCUS       DASVOD010000148         4862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_114350, whole genome shotgun sequence.
ACCESSION   DASVOD010000148 DASVOD010000000
VERSION     DASVOD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114350"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2841
                     /locus_tag="VF720_02985"
     CDS             <1..2841
                     /locus_tag="VF720_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878344.1"
                     /translation="RRNEIITVTALCNGMPIVIGPQNVVLAPGGTTRFSVPCVLPVNC
                     EGDVYVSLTATAAIEGFPDCRKEMTATKPIGCAEGCIDVVACRDEVLACNGSTVNVPF
                     VIRNCTGATEEVVTISATCDGKPVVLPITMYTIGAGDSVTVEVPCRMPDWCMGPVEVK
                     LTANARKSGSIETACPAMDMAICPVQCVEACVTSVSLFEGKVCPGDSVCVPFEVQNCT
                     ETADEVLTFKFKCDGMPIMDLPITLRLGPGQKDTVCVPCRIPDQCDADGFKVVLTTFA
                     SIPGLEACEFKDHAEAILECGTVCVAVNDPVALEGCPGDTVEVPFDVTNCGDVTIDVN
                     MMAACNGMLTDAVDPHHFVLTPGQSIVSTVTCIIPEECKPGEYLTVVNSAWAKGFGGD
                     GGCAMDIAGDTRIYCVPCETEGGCGPIALNAPPGAESCPGDPVSFQFTVENQGQSPVN
                     VAITTPTPGWTFVPNNFNNLATSVPTNVTAYGTAPADYNQSQQLVICAAASFNGVVCD
                     STKDYVNVTAKKILFQVEKTADPDTVTALNSTSRISIRVASYKESGPLNPISVVDRMP
                     EGIAFAGNVSSNCGVMTDAQPGDTVVEFTAFNLAPGTSCTISFDVTCGKDGAFVDTAF
                     VRAYCAGSETSYRELQDIAMVVCKEARSACPRTRGFWRQQTEQKDNGSRKICAADAGD
                     GMDMYRLWRGVIDMTDLTSFKTNDGGTVLVSDLRNLSDADLFDALGCELEGPNPNTQR
                     DQAETQYLALMLNVKMGLIGLDIHVDNGTFMGTVGEAIDAVEAILDNPASTNTQLSNA
                     IDIADKINNRQGLMAQTCPGGENAPFGGFANSCPAGNVGFVCPDEDAPGHVDNTGSPA
                     AGRFSLAAFPNPFNPTARLRWSVPTRMLGQHGVIEIFDAAGRSVAQVFEGTVSSEQNE
                     LVLAPDGWASGMYIARLTVGVESIAYRMVLVK"
     gene            2967..4421
                     /locus_tag="VF720_02990"
     CDS             2967..4421
                     /locus_tag="VF720_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878345.1"
                     /translation="MTPKNATLTAILLLMLPAAPLKAATWTITTIDNGPFLSGYLSMD
                     IDSMNRSQVAYYFGSTVVFETGTLRHAVKNGGTWNLETVDPGASVGQYCTLRLDDQDR
                     PMISYYDNAETQRNLMFAWWAPVGGWTLTKVDTTGATGQHTSLAMRGDGALSIGYFNV
                     DSADFMVARSTLAAARVAGPGPTGAWNVQPVDEIGTVGQFGSAVFNELGGEVFTYYDA
                     SLGDLVSERYLTVGQAWQRTGVDIPGDVGRFVDTLFDPWNFLHVSYYDAGQAKLKYAA
                     ARSGRNAWYSETIPTEPGADVGRPSAIAQNPSGNVEIAYRDGRGWLMLARKVKAYDWI
                     IEPIDQSGTVGEAIDFGIDATGRRHIVFYDATNRRLRYATAAGDPPIVGVPDDPTGGA
                     AVLQLGAVNPNPVTTVTWIPFTTAAPGELELTIVDVSGRRVATPHRGFEAAGAHSIPW
                     DGTDDSGRRLPNGIYFARVTTTTETGTTKLVLAR"
     gene            complement(4457..>4862)
                     /locus_tag="VF720_02995"
     CDS             complement(4457..>4862)
                     /locus_tag="VF720_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878346.1"
                     /translation="SGWETWLRSQRRRVQNLIYESGQYEDGPDPAAGGRATKLNVYAL
                     ESLEHWLSEHPQGMTRARTSLSRAIRLYALRALRGLNPFFWIEWLLFFPRKVAARIGV
                     AGDPEWTDSLRLLYWTALAAALVVFFIFFVMR"
BASE COUNT          868 a         1660 c         1598 g          736 t
ORIGIN      
        1 cggcgcaacg agatcatcac ggtcacggcg ctctgcaacg gcatgccgat cgtcatcgga
       61 ccgcagaacg tggtcctggc gcccggcggc accaccaggt tctcggttcc ctgcgtcctg
      121 ccggtcaatt gcgagggcga cgtgtacgtc tcgctgacgg ccaccgccgc catcgagggc
      181 ttccccgact gcaggaagga gatgacggcc accaagccga tcggctgcgc cgaagggtgc
      241 atcgacgtcg tggcctgccg ggatgaagtc ctggcctgca acggctccac ggtgaacgtg
      301 ccgttcgtca tccggaactg caccggcgcc accgaagagg tggtgacgat cagcgccacc
      361 tgcgacggca agccggtcgt cctgccgatc acgatgtaca cgatcggcgc cggcgattcg
      421 gtcaccgtgg aagtgccgtg caggatgccg gactggtgca tgggaccggt ggaggtcaag
      481 ctcacggcca atgcccgcaa gtcgggcagc atcgagacgg cctgcccggc catggacatg
      541 gcgatctgcc ccgtccagtg cgtcgaggcc tgcgtcacct cggtatcgct gttcgaaggg
      601 aaggtgtgtc ccggcgactc ggtctgcgtt ccgttcgaag tccagaactg caccgagacc
      661 gccgacgagg tcctcacctt caagttcaag tgcgacggca tgccgatcat ggacctgccg
      721 atcacgctca ggctgggccc gggccagaag gacacggtct gcgtgccgtg ccggatcccc
      781 gaccagtgcg acgccgacgg gttcaaggtc gtgctgacca ccttcgcgtc gatccccggc
      841 ctcgaggcgt gcgagttcaa ggatcacgcc gaggccatcc tcgagtgcgg cacggtctgc
      901 gtggccgtga acgacccggt ggccctggaa gggtgccccg gcgacacggt cgaggttccg
      961 ttcgacgtga ccaactgcgg cgacgtgacc atcgacgtca acatgatggc ggcctgcaac
     1021 ggcatgctca ccgacgcggt cgatccccat catttcgtgc tgacgcccgg acagtcgatc
     1081 gtgagcaccg tcacgtgcat catccccgag gagtgcaagc ccggcgagta cctgaccgtg
     1141 gtcaacagcg cctgggccaa gggcttcggt ggtgacggcg gctgcgccat ggacatcgcc
     1201 ggcgacaccc gcatctactg cgtgccgtgc gagaccgaag ggggctgcgg cccgatcgcc
     1261 ctcaacgccc ccccgggcgc cgagagctgc ccgggcgacc cggtttcgtt ccagttcacc
     1321 gtggagaacc agggtcagtc ccccgtgaac gtggccatca ccacgccgac gccggggtgg
     1381 accttcgttc cgaacaactt caacaacctg gccaccagcg tgccgaccaa cgtcacggcc
     1441 tacggcacgg caccggccga ctacaaccag tcgcagcagc tggtgatctg cgcggcggcg
     1501 tcgttcaacg gcgtcgtgtg cgactccacc aaggactacg tcaacgtcac cgcgaagaag
     1561 atcctgttcc aggtggagaa gacggccgat ccggacacgg tgacggccct gaactccacc
     1621 agccggatct cgatccgcgt ggcgagctac aaggagtccg gccccctcaa cccgatcagc
     1681 gtggtcgacc ggatgccgga agggatcgcc tttgccggaa acgtgtcgtc caactgcggc
     1741 gtgatgaccg atgcccagcc cggcgacacc gtcgtcgagt tcaccgcgtt caacctcgcg
     1801 cccgggacgt cgtgcacgat ttcgttcgac gtgacatgcg gcaaggatgg cgcgttcgtc
     1861 gacacggcct tcgtgagggc gtactgcgcc ggctccgaaa cgtcgtaccg cgagctccag
     1921 gacatcgcga tggtcgtctg caaggaggcc cgttcggcct gcccgcggac ccgcggcttc
     1981 tggcgccagc agacggagca gaaggacaac ggcagccgca agatctgcgc ggcggacgcc
     2041 ggtgacggca tggacatgta ccgcctctgg cggggcgtca tcgacatgac cgacctgacc
     2101 tcgttcaaga cgaacgacgg cgggacggtc ctggtgagcg acctccgcaa tctcagcgac
     2161 gccgacctgt tcgacgccct ggggtgcgag ctcgaaggtc ccaatccgaa cacccagcgt
     2221 gaccaggccg aaacgcagta cctggccctc atgctgaacg tgaagatggg gctgatcgga
     2281 ctcgacatcc acgtggacaa cggcacgttc atgggcaccg tcggcgaggc cattgacgcc
     2341 gtcgaggcca tcctggacaa cccggcctcc acgaacaccc agctctcgaa cgccatcgac
     2401 atcgccgaca agatcaacaa ccgccagggc ctgatggccc agacctgtcc cggtggcgag
     2461 aatgcgccgt tcggcggatt cgccaacagc tgtccggcgg gcaacgtcgg cttcgtctgt
     2521 ccggacgagg acgcgccggg tcacgtggac aacacgggat cgccggccgc gggacgtttc
     2581 tcgctggcgg ccttccccaa cccgttcaac ccgacggcgc gcctccggtg gagcgttccg
     2641 actcggatgc tcggccagca cggcgtcatc gagatcttcg atgccgcggg ccggtcggtg
     2701 gcccaggtct tcgagggaac cgtctcgagc gagcagaacg aactggtgct cgcgcccgac
     2761 ggatgggcca gcggcatgta tatcgcccgg ctgacggtcg gcgtggagtc gatcgcctac
     2821 cggatggtgc tcgtgaagta acccgaagaa ccgaccggcc gttccaaagt gcgcggatcg
     2881 gccggtcggg catcgtataa tcgaagccgg tcgggccccg gggttcgacc ggcttcctcg
     2941 ttttgcaccc aacccacgga ccggccatga ccccgaagaa cgccaccctg accgccatcc
     3001 tcctcctgat gctccccgcc gcgccgctca aggcggccac gtggacgatc acgaccatcg
     3061 acaacggccc gttcctgtcg ggttacctct ccatggacat cgactccatg aaccggtcgc
     3121 aagtggccta ctacttcggc agcaccgtgg tcttcgagac cggcacgctc cggcacgccg
     3181 tgaagaacgg cggcacatgg aacctcgaga cggtggatcc gggcgcatcg gtcgggcagt
     3241 actgcacgct ccggctcgac gaccaggacc ggccgatgat cagttactac gacaacgccg
     3301 agacacagcg gaacctcatg ttcgcctggt gggccccggt gggggggtgg acgcttacga
     3361 aggtcgacac gaccggcgcc accggccagc acaccagcct cgccatgcgc ggcgacgggg
     3421 cgctttccat cggctacttc aatgtcgact cggccgactt catggtggcc cgttcaacgc
     3481 tggctgccgc gcgcgtggcc ggccccgggc cgaccggcgc ctggaatgtg cagccggtcg
     3541 acgagatcgg caccgtcggc cagttcggct cggcggtgtt caacgagctg ggcggcgagg
     3601 tgttcaccta ctacgacgcc tcgctgggcg acctcgtctc cgagcgttac ctgacggtcg
     3661 ggcaggcctg gcagcgcacc ggtgtcgaca tcccgggcga tgtcggccga ttcgtcgaca
     3721 cgctcttcga cccgtggaat ttcctccacg tgagctatta cgacgccggc caggcaaagc
     3781 tcaaatacgc ggcggccagg agtggccgga acgcctggta ctcggaaacc attcccaccg
     3841 agccgggtgc cgatgtgggg cgccccagcg ccatcgcgca gaacccctcc ggaaacgtgg
     3901 aaattgccta ccgcgatggg cggggctggc tgatgctggc gcgcaaggtg aaggcctacg
     3961 actggatcat cgagccgatc gaccagtcgg gtacggtggg cgaggcgatc gacttcggaa
     4021 tcgacgcgac tggccgtcga catatcgtct tctacgacgc cacgaaccgg cgcctccgct
     4081 acgccaccgc cgcgggcgat ccccccatcg tgggcgtccc ggacgacccg accggcggcg
     4141 cggccgtcct ccagctcggc gcggtcaatc ccaaccccgt caccaccgtc acctggatcc
     4201 cgtttacgac tgcggcgcca ggcgagctgg agctgacgat cgtcgacgtg agtggccgcc
     4261 gggtggccac gccgcaccgg ggattcgagg cggccggcgc gcactccatc ccctgggatg
     4321 gaaccgatga ttcgggacgc cgactcccga acggcatcta cttcgcgcgg gtcaccacga
     4381 caacggaaac cggaacgacg aaactcgtcc tggcacgttg aggcgcctcg gaagtgactg
     4441 ggccggccgg aaaacctcag cgcatcacga agaagatgaa gaagacgacc agggcggccg
     4501 ccagcgcggt ccagtagagg agccgaagcg agtcggtcca ttccgggtcg ccggccacgc
     4561 cgatgcgagc ggccaccttg cgcggaaaga acaggagcca ctcgatccag aagaagggat
     4621 tgagtccacg gagggcgcgc agcgcgtaca gccggatggc ccgggagagg gacgtgcggg
     4681 cgcgcgtcat cccctggggg tgctcggaga gccagtgctc gaggctctcg agcgcataca
     4741 cgttgagctt ggtggcgcgg ccaccggcgg cgggatcggg gccatcctcg tactgccccg
     4801 attcgtagat caggttctgg acacgtcggc gctggctccg gagccacgtt tcccagccgg
     4861 ac
//
LOCUS       DASVOD010000149         6700 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_61984, whole genome shotgun sequence.
ACCESSION   DASVOD010000149 DASVOD010000000
VERSION     DASVOD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61984"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..705
                     /locus_tag="VF720_03000"
     CDS             133..705
                     /locus_tag="VF720_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEX7878347.1"
                     /translation="MTANDPAPDSTPHGAGSDAFFAQVYDELRAIAHARMRGERAGHT
                     LQSTELVHEAYMRLKPGDGSTWENRAHFFAAAAEAMRRILIERARARGRVKRGGDGEG
                     RPAPRVSLNLDEVAALADDQDPETVIALDDAIDRLSENDERAARIVRLRFYAGLSVEE
                     VAEILEASPRTVLRDWAFARAWLYGELSRG"
     gene            complement(681..3536)
                     /locus_tag="VF720_03005"
     CDS             complement(681..3536)
                     /locus_tag="VF720_03005"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878348.1"
                     /translation="MANARWSRLRNLFEQARELTGEERSALLDRELADDAVLRAELDS
                     LLKAGDDASHFLVDHAALGTGSSIGPYHLIEPIGEGGFGVVYLAEQQRPIKRRVALKL
                     IKPGMDSRQVIARFEAERQALALMDHPGIAQVFDAGETPEGRPYFAMEHVAGVPITVF
                     CDQEKLSLRERLEVFLLACDAIRHAHQKGVIHRDIKPSNVMVARRDGSPALKVIDFGI
                     AKATGENSPGGPTMTREGMIIGTTGYMSPEQLGATNAPVDTRSDIYSLGVLLYELLAG
                     DLPYDREQLKRASWLDAMQIILGDPTPPAVRAARTDTAEVAVKRSTDARSLVKSLRGE
                     LEWITLKALEKEPERRYASASELANDIRHFLADEPVSAAAPGTMYRIGKYARRHRVGV
                     TAAALILLAIVAGGIAATVGFGRAVKAERLAKREAESSAQVADFLVGLFHKSSPGAGG
                     DTMTVRSLLDEGVRKIEMDPPDDPHVRARLFGAMSDSYLNLGQYDEGVRLTRAALAAI
                     ESAKPRDDVEVARYLDKVANGLSMAGEEESLLVVVERTIALLSANRTGDPDLLSWCLY
                     RKARYRLNHGGVEEADSLIDLAIATAKSVPAPDNERLARMYGTKGLAASWRYDFASAI
                     TSYRTGLECARRAGEPMRIAAMEQAIAGAFEGQGEGDSSLAHAEAGVAIARRVYAPDH
                     PALAGALGAQTGALSSLGRYPEAIALQEEVVRILRGRGKPGEQLAYELAILGSYLQMD
                     GKLEEAIERTREAVDLYESDLGPMHFRVGETKANLARYEMEIGRMDAAEAHFRQTIEI
                     GTAIDDKTIVLPIARQDFGNLCIDLGRLAEAESLFARSSAGLDSTNNALRAYYGNNII
                     GLARIWARQGRVAEAESLMAHGVRLRLEDVPEADPSLVDLWLCLAEVRWMTGHPEEAI
                     ESLRRAGKSGAASADVARYPELAALRSRADYPLDNSP"
     gene            complement(3615..5393)
                     /locus_tag="VF720_03010"
     CDS             complement(3615..5393)
                     /locus_tag="VF720_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX7878349.1"
                     /translation="MSTSRVCIAALAVLVMVLLGPALAAAQCGPWRMDGCLKDITVVG
                     CEPGGAHVDYVPPTAHNDCGPIPAILYYGPPPGSLFPFGTTTVEYWTGLDENGNFDSC
                     VFDVIVLAPERHITQGKNITVTGCDPGGAVVTYPMPTAWDDCGPLTPELISGYPPGSV
                     FPFGTTTVEWWTNTNPDGQWETTVFEVNVLVPEKQITCVNNIFVTGCVGGAVITYEEP
                     VITDACGTRPAELYQGYRSGSVFPTGPTTVEWWSGLDANNEFVTCVFEVTVDDDTKPP
                     VIDCPEIVVATAPAGQSSAIVHFDVSATDSCGGAVTLDFQPPDGSSFPCGTTPVTVTA
                     RDPMGNIDTCTFDVTVASTVPLDVRPGDCPNQLNLNANAAVPVAIMGSSLFDIARIDP
                     ASLRLEGVAPMRWSLDDVGAPYSPFTGKDDCEDCNRAKKDRIADFSMKFDGAELREAM
                     GDAMPGECRVLRLTGQTIDGCPIAGEDVVRIKHIANAAISIEPGTSPVGTALHAIRPN
                     PVVNAAGFAWEMETAAHASITIFDIAGRRVRQLADGWQGAGQHEAEWDVRDELGMPVP
                     NGVYLYALTVDDGTGRRFVRKLIVSR"
     gene            complement(5526..5759)
                     /locus_tag="VF720_03015"
     CDS             complement(5526..5759)
                     /locus_tag="VF720_03015"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7878350.1"
                     /translation="MARAIFDLRSGAGLTQAALAKRIGTSPSVISRLEDADYSGHSLA
                     LLRRIAEALDRQLEVQFVPRRRPRTLTARRPPG"
     gene            complement(5650..6246)
                     /locus_tag="VF720_03020"
     CDS             complement(5650..6246)
                     /locus_tag="VF720_03020"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7878351.1"
                     /translation="MPQTRVILYRDDDGIVPLIKWLDRLQPKAQLKCLASLKRLEDMG
                     YELRRPEADYLRDDIHELRIRFGRVNLRMLYFFHGRDAVVVSHGLSKQDKVPRRDIEL
                     AKRRRAQFESDPLKHTFSEQSNHVQEASIRIPGAPTPRSSLHRQERTTAGGVRGGSPE
                     FPGGEGHLRSSLRCGPDTGCSRETDRHVTLRHLAARGC"
     gene            6472..>6700
                     /locus_tag="VF720_03025"
     CDS             6472..>6700
                     /locus_tag="VF720_03025"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEX7878352.1"
                     /translation="MKSRLTLSLEESLVKRATSYARRHGLSLSEVVRRCFEVLPGEDP
                     DPELTPTVRSLYGALSPSNLKRRKYRKHLERK"
BASE COUNT         1133 a         2362 c         2098 g         1107 t
ORIGIN      
        1 cctgctgcag cgcaagttcg gatgcctgcc ggccgggccg gcagaaccgc cgcaaccggg
       61 cgatcccccc tccgtgcgcc gaaacccgtc cggaggtgac agggcccgcc ggcaccgtta
      121 ggctcgaccc cgatgaccgc caacgaccct gcccccgact ccacgcccca cggcgccggt
      181 tccgacgcct tcttcgcgca ggtctacgac gagctgcgcg ccatcgcgca cgcccgcatg
      241 cggggcgaac gggccgggca cacgctgcaa tccaccgagc tggtccatga ggcgtacatg
      301 cgcctcaagc cgggagacgg cagcacctgg gaaaaccgcg cccacttctt cgcggcggcc
      361 gccgaggcca tgcggcgcat cctcatcgag cgcgctcggg ccaggggacg ggtcaagcgg
      421 ggtggcgacg gcgagggaag gcccgccccc cgcgtgtcgc tcaacctcga cgaagtggcg
      481 gcgctggccg acgaccagga tcccgaaacc gtcatcgcgc tcgatgacgc gatcgaccgg
      541 ctctccgaaa acgacgaacg ggccgcgcgc atcgtgcggc tccgcttcta cgcgggcctc
      601 agcgtggagg aggtggcgga gatcctggaa gcctcaccac gcaccgtgct gcgcgactgg
      661 gccttcgcgc gcgcctggct ctacggggag ttgtcgaggg ggtagtccgc gcgcgagcgg
      721 agcgcggcca gctccgggta tcgcgccaca tcggcggatg cggcaccgga cttccccgcg
      781 cgtcgcagac tttcgatggc ctcctcggga tgcccggtca tccagcgcac ctccgccagg
      841 cagagccaca ggtccaccag cgaaggatcg gcctcgggca catcctcgag ccggagccgc
      901 acgccgtgcg ccatcaacga ctcggcctcc gcgacacgac cctgtcgagc ccagatccgc
      961 gccaacccga tgatgttgtt gccgtagtag gcgcgcaggg cgttgttggt cgaatcgagc
     1021 cccgcggacg agcgggcgaa gaggctctcg gcctcggcca atcgtcccag gtcgatgcaa
     1081 aggttgccga agtcctgccg cgcgatgggt aacacgatcg tcttgtcgtc gatcgcggtg
     1141 ccgatttcga tcgtctggcg gaagtgggcc tcggccgcgt ccatccggcc aatctccatc
     1201 tcgtagcggg ccaggtttgc cttggtttcc ccgacgcgga agtgcatggg accgaggtcc
     1261 gattcgtaca ggtcgacggc ctcccgcgtc cgctcgatcg cctcctcgag cttgccatcc
     1321 atctgcaggt agctgccgag tatcgccaac tcgtacgcca gctgttcccc cggttttcca
     1381 cggccgcgca ggatccgcac gacctcctcc tgcagggcga tggcttcggg ataacgacca
     1441 agggaactca gcgctccggt ctgtgcgccc aacgcgccgg caagcgccgg gtggtcggga
     1501 gcgtacacgc gacgcgcaat cgccacgccc gcctcggcat gcgccagcga cgaatcgccc
     1561 tctccctgcc cctcgaacgc gccggcgatg gcctgttcca tggcggcgat gcgcatcggc
     1621 tcaccggccc gccgcgcgca ctcgagtccg gtccggtagg acgttatcgc ggaggcgaag
     1681 tcgtaccgcc aggatgccgc cagccccttg gtgccgtaca tgcgggccag gcgttcgttg
     1741 tcgggagccg gcacgctctt cgccgtcgca atggccaggt cgatcagcga gtcggcctcc
     1801 tcgacgccgc catggttcaa ccggtagcgt gctttccggt acaggcacca tgacaacagg
     1861 tcgggatcgc cggtccggtt ggccgacagg agcgcgatgg tgcgctccac caccacgaga
     1921 aggctctcct cctcgcccgc catgctcagt ccgttggcca ccttgtcgag ataacgcgcc
     1981 acttccacgt catccctcgg cttcgctgac tcgatcgccg ccagcgccgc ccgcgtcagt
     2041 cttacccctt cgtcgtactg ccccaggttc aggtacgaat cactcatcgc gccgaacagg
     2101 cgcgcccgca catgcggatc gtcgggcgga tccatctcga tcttccgcac gccctcgtcg
     2161 aggagcgagc gcacggtcat cgtgtcgccc cccgcgcccg ggctcgactt gtggaagagc
     2221 cctaccagga agtcggccac ctgtgccgat gactcggcct cccgcttcgc cagccgttcg
     2281 gccttcacgg cccgcccgaa tcccaccgtc gccgcaattc cgcccgccac gatggccaac
     2341 aggatcagcg ccgccgccgt cacccccacc cgatgccgcc gcgcatactt cccgatccgg
     2401 tacatcgtgc ccggcgccgc cgccgacacc ggctcatcgg ccaggaagtg ccggatgtcg
     2461 ttggccagct ccgacgccga cgcgtagcgc cgctccggct ctttctcgag agccttgagc
     2521 gtgatccact ccagctcccc ccggagcgac ttcacgaggc tccgggcgtc ggtggaacgc
     2581 ttcaccgcca cctcggccgt gtccgtcctg gccgcgcgca cggccggcgg cgtcggatcg
     2641 ccaaggatga tctgcatggc gtcgagccac gacgccctct tcaactgctc gcggtcgtaa
     2701 ggcaggtcgc cggccagcag ttcatacagg agcacaccaa gggagtagat gtccgagcgc
     2761 gtgtccaccg gggcgttggt ggccccgagc tgctccgggc tcatgtagcc cgtcgtgccg
     2821 atgatcatcc cctcgcgcgt catcgtcggc ccgcccggac tgttctcgcc cgtggccttg
     2881 gcaattccga aatcgatgac tttgagtgcc ggcgacccgt cccgccgcgc caccatcaca
     2941 ttcgacggct tgatatcgcg atggatcacg cccttctggt gcgcatgccg gatggcgtcg
     3001 cacgccagca ggaacacctc gagccgctcc cgcagcgaca atttttcctg gtcgcagaac
     3061 accgtgatcg gcacacccgc cacgtgctcc atggcaaagt acggccgccc ctccggcgtt
     3121 tcccccgcat cgaacacctg ggcaatgccc gggtgatcca tcagggccag cgcctgccgc
     3181 tcggcctcga accgggcgat gacctgccgt gaatccatgc ccggcttgat gagcttgagg
     3241 gccacgcggc gtttgatcgg ccgctgctgc tccgccaggt acacgacgcc gaagccgccc
     3301 tcaccgatcg gttcgatcag gtgatacggc ccgatggagg atcccgtccc gagcgccgcg
     3361 tgatccacca ggaagtggct ggcgtcatcg ccggctttca ggagtgaatc gagctcggcg
     3421 cgcagcacgg catcatccgc cagctcacga tcgagcaacg cggaccgctc ctcgcccgtc
     3481 agctcccggg cctgctcgaa caggttgcgc aggcgactcc accgcgcatt ggccatcgct
     3541 cgctcctccg agggcgccgg aggacggcgc ccggacacgc agtgtacacg ggcgccgctc
     3601 cggcgcggtg tgccctaccg gctgacgatc agcttccgga cgaagcgccg ccccgtaccg
     3661 tcatccaccg tcaacgcata gaggtacacg ccgttcggca ccggcatccc caactcgtcg
     3721 cgaacatccc attccgcctc gtgctgaccg gcgccctgcc acccgtcggc gagttgccgt
     3781 acccggcggc cggcgatgtc gaagatcgtg atcgaagcgt gcgccgccgt ttccatctcc
     3841 caggcgaacc cggccgcgtt gacgaccggg ttcggacgaa tagcgtggag cgcggtgccc
     3901 acgggtgagg tccccggctc gatcgagatc gccgcattcg cgatgtgctt gatccgcacc
     3961 acgtcctcgc cggcgatcgg acaaccgtcg atcgtctgtc ccgtcaggcg cagcacgcga
     4021 cattcaccgg gcatcgcatc gcccatggcc tcccggagct ccgcgccgtc gaatttcatc
     4081 gagaaatccg cgatgcggtc cttcttcgcc cggttgcagt cctcgcaatc gtcctttccc
     4141 gtgaacggcg aatacggtgc acccacgtcg tccaggctcc atcgcatcgg cgccaccccc
     4201 tcgagccgga ggctggccgg gtcgatccgc gcgatgtcga acaggctcga ccccatgatc
     4261 gccaccggca ccgctgcgtt ggcattgagg ttcagctggt tcggacagtc acccgggcgc
     4321 acgtcgagcg gcaccgtcga ggccaccgtc acgtcgaacg tgcacgtgtc gatgttcccc
     4381 atcgggtcgc gggccgtcac ggtaaccggc gtcgttccgc agggaaagct ggatccgtcc
     4441 ggtggctgga agtcgagcgt cacggcaccg ccgcacgaat ccgtcgccga gacgtcgaag
     4501 tgcacgatcg ccgagctctg ccccgccggt gccgtcgcca ccacgatttc cggacagtcg
     4561 atgaccggcg gtttcgtgtc gtcatccacc gtcacttcga agacgcacgt cacgaactcg
     4621 ttgttggcgt cgagcccgct ccaccattcc acggtcgtcg gccctgtcgg aaacacgctc
     4681 cccgagcgat agccctggta cagctcggcc ggccgcgtcc cgcaggcatc ggtgatgacc
     4741 ggctcctcgt aggtgatgac ggcgccgccc acgcagcccg tcacgaagat gttgttcacg
     4801 caggtgatct gtttctccgg cacgagcacg ttcacctcga aaacggtcgt ttcccactgc
     4861 ccgtccggat tggtgttggt ccaccactcc acggtcgtgg tgccgaaggg gaacacactc
     4921 cccggcgggt acccgctgat gagctccggc gtcagcgggc cgcaatcgtc ccaagcggta
     4981 ggcatcggat aagtcaccac ggcgccgccg gggtcgcagc cggtcacggt gatgttcttc
     5041 ccttgcgtga tgtgccgttc cggcgccagc acgatcacat cgaagacaca ggagtcgaaa
     5101 tttccattct cgtcgagacc cgtccagtac tcgacggtcg tcgtcccgaa cggaaacagg
     5161 cttcccggcg gcggtccgta atacaggatc gccggaatcg gcccgcaatc gttgtgcgcc
     5221 gtcggcggca catagtccac gtgggccccg cccggctcgc acccgacaac ggtgatgtcc
     5281 ttcaggcaac catccatccg ccagggaccg cactgggcgg cggcaagcgc aggtccaagc
     5341 agaaccatca ccaggacggc gagggcagcg atgcaaacgc gggatgtcga cacgggagcc
     5401 tcctgtcatc acggcactta cgagccggag cagccctccg gccggaccgc cgcggacggg
     5461 cggtcgaggc tacgtgcgac ggaaggacgg gaggggtgac actctcgccg gcaccggtcg
     5521 ccgcgtcatc cgggcgggcg gcgagccgtt agcgtccgcg gacgccgacg gggcacgaac
     5581 tgaacctcaa gttgtcgatc caacgcctca gcgatccggc ggagcagagc aagcgaatgg
     5641 ccgctgtagt cagcatcctc gagccgcgag atgacggagg gtgacgtgcc gatccgtttc
     5701 gcgagagcag cctgtgtcag gcccgcaccg gagcgaagat cgaagatggc cctcgccacc
     5761 tgggaactct ggactgccgc ctcgaacgcc ccctgccgtc gttcgttcct gccgatgtaa
     5821 cgagcttcga ggtgtcggag cgccggggat tcgaatcgat gcttcctgga catggttact
     5881 ctgctccgag aaggtgtgct tcaaagggtc ggactcgaat tgggcccttc gtcgtttggc
     5941 gagctcaata tcccgacggg gaaccttgtc ctgttttgaa agaccgtgtg agacgaccac
     6001 ggcatccctg ccgtgaaaga agtagagcat gcgtagattc acccgcccga acctgattcg
     6061 aagttcgtgg atatcatcac gcagatagtc ggcttcaggc cggcgcagtt cgtaccccat
     6121 atcctcgagg cgcttgagag acgcgaggca cttcaactgg gccttcggct ggagcctatc
     6181 gagccacttg atcaacggta cgattccatc gtcgtcacgg tagagaatga ccctggtttg
     6241 cggcacaacc tctcccagta tatcgcaaaa ttgcgaatat gacgggagac aaatcagcgt
     6301 gacggggaga acatctcagc agattccggt tcgactcgac tagtgggacc gactcgccga
     6361 accgtggcag gcgccacagg gttgggcgca cgggcgagaa tgtagaccgt tggaatgtcg
     6421 ggtcggcgtg gggcagtatc tgctgagcaa ttgtgcagaa cggaggaaag aatgaagtcg
     6481 agactgacac tgagcctcga agagtcgctc gttaagaggg cgacatccta cgcacgcagg
     6541 catggccttt cgctctcgga agtggtgagg cggtgcttcg aagtcctccc gggagaggat
     6601 cctgatccgg agctgacccc gaccgtgcgg tcactctatg gcgcgctgag tccgtcgaat
     6661 ctcaaacgca ggaagtaccg gaagcatctc gagagaaagt
//
LOCUS       DASVOD010000150         6355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_844424, whole genome shotgun sequence.
ACCESSION   DASVOD010000150 DASVOD010000000
VERSION     DASVOD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844424"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..1758
                     /locus_tag="VF720_03030"
     CDS             43..1758
                     /locus_tag="VF720_03030"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX7878353.1"
                     /translation="MRIAAGPATTLLFLVFHLVGAPRASHAQADSTDAPRTLDISPAA
                     PDAVPPPPKPILSGTVPADDDIYRDLERLDALGLIPASVLGQRPLSRAEIARILRQAS
                     NNPRAAREPVRRLLIRLRKEYPWQEGVEPRGVDEVRYGAAWLEDEPVRFLRDNGVGQL
                     DALNQPLTSDHNGRYYRSNGGTGWLLTRHTLPFRTNLVAVLAPEFLVRFGNYDQGNVP
                     NADSTDTRESDVQGDFTFRSIYGRLGIGPAIVQVGRDPLAWGPSPSGSLLLSTNARPL
                     DMAYLTTDSPFRLPWFFRGLGPSRWFIGMAALGQDRVLPGSALALLRASFRVHRNIEL
                     GFSESLVMLGDDSPTDDFWQYLWEFFPAGRLGTDSDLADHRFGFDSRWHIWPGHLSLY
                     GEILVEDVRANHYQDVTARRLGVYLPAIGPGGQWEARGEFLRLPAILYRHGRWTTGYA
                     LNGHLLGNEIGPDSRGLRFRFEKTGNEGQRTLLEFAWESRDSDIWAQEVAENGDNEDI
                     YRAVDRPSEMRWRSRLGFNWPLTPHVKCLPNFAVERVTRAEGEMGNDRTDFMVELAFR
                     YAFER"
     gene            complement(1759..4530)
                     /locus_tag="VF720_03035"
     CDS             complement(1759..4530)
                     /locus_tag="VF720_03035"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878354.1"
                     /translation="MGRRGVLFLAAIVLLANLPSSSLAAFVNFESHQIHPIGMSPDGS
                     KLAALNTPDGYLVIYELSIVTGPTMTAQIPVGMEPVSLTWHGNNEIWVANHLSDAISI
                     VNVTTGNVVKTLLVGDEPTDVVFAGAPERAFVCVSQEDAVKVYDPANLAAAPIVIPIF
                     GSDPRALATSPDRSKVYVSVFESGNRSTILPETTVVNGGGQPPANPPMDPMLPPAPNT
                     GLIVQKSGANWVDDTGGIWNSHVTWSLPDNDIVQISTNTMTVTANFTGVGTLNYDLEV
                     NPSSGVIYVSNHESLNLTRFENNLQDNFARMRVTIINPLGPTITPIHLNPHINYAVVP
                     GPQSEMDQSLSLPTDMVWNSSGTLLYMAAMGSGKVAVLTPGGTVVDRWDVADGPTGLA
                     LDENRGRLYVLDRFAEKIRCVSLSTGTVVQLAGLGFNPEPPEVQNGRKFLYDARLSSG
                     HGDVSCATCHPFANFDGLAWDLGRPDGAYLSAPDGQLDTLLVGFHPMKGPMVTQSLRG
                     LRDTAPYHQRGDRGDLFEFNVAFPDLMGRASGLDGADMSAFNDFIQTVTYPPNPNQNL
                     DRTLPNPTLQPSPQRGRNAFTTLHLDDGKFCVECHEFPAGTNNQITSATVMGNTQDLN
                     ISQLRNLYEKTGYGRTGALKNGFGFTHNGQWETLFEFFKQPFFTFGSNLVRRDMEAFM
                     MAFDTDTAPAVGAQQTIDAANKNDSAVVTRISTLITQADIGNIDLMVKGKVGGLARGY
                     RYIGGNQFESDALGDGFVHKDTLHAWAGAGSELTYTGLPPGCGMKAIDRDGDSFRDRD
                     ELNAGSDPANPESTPATGGVADPTPVGGERLLGGLVSRFVATPNPVRQSGTRIEFQLT
                     RSADVTLRLFDPQGRLIRTLVDGPRDGAVSVDWDLTNQSGQRMPSGVYFYRLESGSFF
                     ETRRLTILR"
     gene            5048..5467
                     /gene="mce"
                     /locus_tag="VF720_03040"
     CDS             5048..5467
                     /gene="mce"
                     /locus_tag="VF720_03040"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEX7878355.1"
                     /translation="MKAQLDHVGIAVRSLAEAIPAFEAALGATASQTEEVADQDVRVV
                     FLKTGEPAIELLEPIGPEGPIGKFIQKRGEGIHHLSFDVPDLEAALARCKAAGLTLID
                     EQPRKGAAGKRIAFLHPRSTNGVLIELSESNTDRSSS"
     gene            5549..5636
                     /locus_tag="VF720_03045"
     tRNA            5549..5636
                     /locus_tag="VF720_03045"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5583..5585,aa:Ser,seq:tga)
     gene            complement(5762..6295)
                     /locus_tag="VF720_03050"
     CDS             complement(5762..6295)
                     /locus_tag="VF720_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878356.1"
                     /translation="MKTRSALLSVVACLLLAAPVMADVVVLEPVQTAVTEEEGTARVA
                     VEFDLSGLRSGDGRCIDIATLTWSVSGAPEAEIAYLVSYPIDAAWHAQEVSAELEELD
                     VGETAAASWEITPEEHENGLGDIVRLDLTGLASTWAGNGAGNHGVVIETGDIAAADLE
                     AALSTLRLTVHYGFRAE"
BASE COUNT         1109 a         2183 c         1976 g         1087 t
ORIGIN      
        1 cccggcttga accgcaccac gcgttgaccc catcctcccg cgatgcgcat cgccgcggga
       61 cccgccacga ccctcctctt cctcgttttc cacctggtcg gtgcgccgcg agcgtcccac
      121 gcccaggccg attccaccga cgcaccccgc acgctcgaca tctcccccgc cgcgccggat
      181 gcggtcccgc cgccaccgaa accgatcctt tccggcacgg tgcccgccga cgacgacatc
      241 taccgcgacc tcgagcgcct cgacgccctc ggcctcatcc cggcttccgt gctcggccag
      301 cgccccctct cgcgcgccga gatcgcccgg atcctgcgac aggcttcgaa caatcctcgc
      361 gccgctcgcg agccggtgcg ccggctcctg atccggctgc gcaaggagta tccgtggcag
      421 gaaggcgtgg agcccagggg cgtggatgaa gtgcgctacg gcgccgcctg gctcgaagac
      481 gagccggtcc gcttcctccg ggacaacggc gtcggccagc tcgacgccct gaaccagccg
      541 ctcacttccg atcacaatgg ccgttactac cgctcgaacg gcggcaccgg gtggctcctc
      601 acgcgacaca cgctgccgtt ccgcaccaac ctcgtggccg tcctcgcgcc ggagttcctc
      661 gtccgtttcg gcaactacga ccagggaaac gtgccgaacg ccgattccac cgacacgcgc
      721 gaatcggacg tgcagggaga cttcacgttc cgttcgatct acggccgcct tggcatcggc
      781 ccggccatcg tccaggtggg gcgcgatccg ctggcctggg gcccatcacc gtcggggagc
      841 ctgctacttt cgacgaacgc gcgcccgctc gacatggcct acctcaccac cgactccccg
      901 tttcgcctgc cgtggttctt ccgcgggctc ggcccgagcc gctggttcat cgggatggcg
      961 gcgctcggcc aggatcgcgt gctccccggg agcgcgctgg cgctgctccg cgcctccttc
     1021 cgcgtgcatc gcaacatcga gctcggcttc agcgaatcgc tcgtgatgct tggcgacgac
     1081 agccccaccg acgacttctg gcagtacctc tgggagttct tcccggccgg ccgcctcggc
     1141 accgattcgg acctggccga ccatcgcttc ggtttcgaca gccgctggca catctggccg
     1201 gggcacctct cgctgtacgg cgaaatcctg gtggaggatg tgcgcgcgaa ccactaccag
     1261 gatgtcacgg cccggcgcct gggtgtgtac ctgccggcca tcggccccgg cgggcagtgg
     1321 gaggcccgcg gcgagttcct tcgcctgccg gccatcctct accggcacgg tcgctggacg
     1381 acgggctacg cgctgaacgg ccacctgctc ggcaacgaga tcggcccgga ctcgcgcggc
     1441 ctccgcttcc gctttgaaaa gaccgggaac gaaggtcagc gcacgctgct cgagttcgcg
     1501 tgggaaagcc gcgacagcga catctgggcc caggaagtgg ccgagaacgg ggacaacgag
     1561 gacatctacc gcgcggtcga ccggccgtcg gaaatgcgct ggcgcagccg gctcggcttc
     1621 aactggccgc tcaccccgca cgtgaagtgc ctgccgaact tcgcggtcga acgcgtcact
     1681 cgggcggaag gggaaatggg gaacgaccgc accgacttca tggtggaact ggccttccgc
     1741 tacgccttcg agcgctgatc agcgcaggat cgtcagccgc cgcgtctcga agaacgaacc
     1801 cgactccagg cgatagaagt acacacccga aggcatccgc tgccccgact ggttggtcag
     1861 gtcccaatcg acgctcacgg cgccatcgcg cggcccatcc accagcgtgc ggatcaaccg
     1921 cccctgcgga tcgaacagcc tgagcgtgac gtcggccgac cgcgtgagct ggaactcgat
     1981 ccgcgtaccc gactgccgca ccgggttcgg cgtggccacg aagcgcgaga caagccctcc
     2041 gagcaaacgt tcaccgccaa ccggcgtcgg gtcggccaca ccgccggtgg cgggcgtcga
     2101 ttcgggattg gccggatcgg agccggcgtt caattcgtcg cgatctcgga agctgtcgcc
     2161 gtctcgatcg atggccttca tgccgcaacc gggtggcaga ccggtgtagg taagctcgga
     2221 gccggcgccg gcccaggcat gcagcgtgtc cttgtgcacg aagccgtcgc ccagcgcgtc
     2281 ggattcgaac tggttgccgc cgatgtaacg gtagccgcgg gccagcccgc ccaccttccc
     2341 cttcaccatc aggtcgatgt tcccgatgtc ggcctgcgtg atgagcgtgg aaatccgcgt
     2401 cacaacggcc gagtcgttct tgttggcggc gtcgatggtc tgctgggcgc cgacggccgg
     2461 cgccgtgtcg gtatcgaagg ccatcatgaa ggcctccatg tcccggcgca ccaggttcga
     2521 gccgaaggtg aagaaaggct gcttgaagaa ctcgaagagc gtttcccact gcccgttgtg
     2581 cgtgaagccg aacccgttct tgagcgcccc ggtccggccg tagccggtct tctcgtacag
     2641 gttgcgcagc tggctgatgt tcaggtcctg ggtgttcccc atgaccgtgg cgctggtgat
     2701 ctggttgttc gtgccggccg ggaactcgtg gcactccacg cagaacttgc cgtcatccag
     2761 gtgcagcgtg gtgaaggcat tccggccgcg ctgcgggctc ggctgcagcg tggggttcgg
     2821 gagggtgcga tcaaggttct ggttgggatt gggcggatag gtaaccgtct ggatgaagtc
     2881 gttgaaggcg gacatgtcgg ccccgtccag acccgacgcc ctccccatca aatccgggaa
     2941 cgccacgttg aactcgaaga ggtcgccacg gtcgccgcgc tggtggtacg gcgcggtatc
     3001 gcgcaggccc cgcaacgact gcgtgaccat cggccctttc atcgggtgga agccgacgag
     3061 cagggtgtcg agctggccat ccggcgccga caggtaggcg ccatccggcc gccccaggtc
     3121 ccaggccagt ccgtcgaagt tggcgaaagg atggcaggtg gcgcacgaca cgtccccgtg
     3181 gcccgacgac aggcgggcgt cgtacaggaa cttgcgccca ttctggactt ccggcggctc
     3241 gggattgaag ccgagtcccg ccagctgcac gaccgttccg gtcgagagcg atacgcagcg
     3301 gatcttctcc gcaaagcggt cgagcacgta gaggcgcccg cggttctcgt cgagtgcgag
     3361 gccggtcgga ccatcggcca cgtcccaccg gtccaccacg gtgccgcccg gcgtgagcac
     3421 cgccaccttg ccggagccca tggcggccat gtagaggagc gtgccgctgg aattccagac
     3481 catgtcggtc ggaagcgaaa ggctctggtc catctcgctc tgcggacccg gcaccaccgc
     3541 gtagttgatg tgcggattca ggtggatggg cgtgatcgtc gggccgagcg ggttgatgat
     3601 ggtcacgcgc atccgcgcga agttgtcctg caggttgttc tcgaatcgcg tgaggttcag
     3661 cgattcgtgg ttgctcacgt agatgacgcc cgagctcggg ttgacctcca ggtcgtagtt
     3721 gagcgtcccc acacccgtga aattggcggt gacggtcatc gtgttggtgc tgatctgcac
     3781 gatgtcgttg tcgggcagcg accaggtcac gtgcgagttc cagatgccac cggtgtcatc
     3841 cacccagttg gcccccgact tctgaacgat gagcccggta ttgggcgccg ggggaagcat
     3901 cgggtccatc ggcgggttgg ccggcggctg cccgcctccg ttcaccacgg tggtctcggg
     3961 caggatggtg gaacggttgc ccgactcgaa cacgctcacg tacaccttgg agcggtcggg
     4021 gctggtggcc agcgcccggg gatccgaacc gaagatcggg atgacgatcg gagccgccgc
     4081 caggttggcc gggtcgtaca ccttcacggc atcttcctgg ctcacgcaca cgaaggcccg
     4141 ctcgggcgcg ccggcaaaga ccacgtcggt cggctcatca cccacgagca gcgtcttcac
     4201 gacgttgccg gtggtgacat tgacgatgct gatggcgtcg gaaaggtggt tggccaccca
     4261 gatctcgttg ttcccgtgcc aggtcaggct gacgggctcc attcccaccg ggatctgggc
     4321 cgtcatggtg ggcccggtga cgatgctcag ttcataaatg accaggtagc cgtcgggcgt
     4381 gttgagggcc gccagcttgc tgccatccgg cgacatgccg atcggatgga tctggtgact
     4441 ctcgaaattg acgaacgcgg cgagggaact actgggaagg tttgcaagga gcacaatggc
     4501 cgccaggaac aacacaccac gacgacccat ccgtcttgcg acacccgcgg ccaaaacaga
     4561 catcactgcc ctccgtcacc attcccccct gactgtggaa cgggggggtt gcgctttgaa
     4621 ttggggtgcg aacggctggc tcaagccgct gacatgtcga tcggaagcca caatagtatc
     4681 aaacaagact ctcactatag aaggaaggaa ttccgtgccc gaaagacccg actgcaggcc
     4741 taactatttg taaataaata atttacacat atttctcagg atggtgactg gccgatcagc
     4801 aacggcgcgg gcggccaggg cggtgcgatc aacacgcgcc cggggcgtcc acgccgtcac
     4861 ccaagcccgt aacaggcggc cggggagcgc gggtctttgc aagccgtgtg ccccggcgcc
     4921 aacccccgca actgacgttg tcatcgcgcg acgcgcgtcc tcacgatcag gttcttgacg
     4981 acatgtcgtc accgatgtga cgacgcccac accacacgct ctccgaccat cggctagaat
     5041 gcgaaccatg aaagcccaac tcgatcacgt cggcatcgcg gtccgctcgc tggccgaagc
     5101 catccccgcc ttcgaagccg ccctcggcgc caccgcatcc caaaccgaag aagtggccga
     5161 ccaggacgtc cgcgtcgtct tcctcaagac cggcgaaccc gccatcgagc tcctcgagcc
     5221 catcggcccc gaaggcccca taggaaaatt catccagaag cgcggcgaag gaatccacca
     5281 cctcagcttc gacgttcccg acctcgaagc cgccctcgcc cgctgcaagg ccgccggcct
     5341 gaccctgatc gacgaacagc cgagaaaagg cgccgccggc aagcgcatcg ccttcctcca
     5401 ccccaggtcg acgaacggcg tcctgatcga actctccgaa tcaaataccg atcgatcatc
     5461 aagctaaaaa attcccgcga ccggccgccc cgcgcacgcc cggtcgtggt gatccacatt
     5521 tcttttcgac atttcggttg caacgcgcgg agggatgtcc gagtggttta aggaaccggt
     5581 cttgaaaacc ggcgtggtga aagccaccgt gggttcgaat cccactccct ccgccaggtt
     5641 acgggattca ggcaaatcgt gctgtgccga ccttcaccaa ttaattcgcg attaattctc
     5701 gaacttccat gagaaagccc ggtgctgcca tcgctgacga caccgggctt tggacgatct
     5761 gctactcggc cctgaatccg taatgcaccg tcagccggag cgtcgacagc gcggcctcga
     5821 gatccgccgc cgcaatgtcc ccagtctcga tcacgacgcc atggttgccg gcgccgttgc
     5881 ccgcccaggt ggatgccaga ccggtcagat caagccggac gatgtcaccg agaccgttct
     5941 catgctcctc cggcgtgatt tcccagctcg cggccgcggt ctctccgaca tccagttcct
     6001 cgagctcggc cgagacttcc tgggcgtgcc aggccgcgtc gatcgggtac gagacgaggt
     6061 aggcaatctc cgcttcggga gcaccgctca ccgaccaggt gagcgtggcg atgtcgatac
     6121 agcgcccatc gccgcttcga agtccggaga ggtcgaactc gacggccacc cgcgccgtcc
     6181 cttcctcttc tgttaccgca gtctgcaccg gttcgagaac caccacgtcg gccattacgg
     6241 gcgccgccag aagaaggcaa gccaccacac tcaacagagc acttctcgtc ttcatggcac
     6301 tctcctccgt cagcggacgt agacgaactt ggtgattccg agcacgtccc ggtca
//
LOCUS       DASVOD010000151         3381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_479658, whole genome shotgun sequence.
ACCESSION   DASVOD010000151 DASVOD010000000
VERSION     DASVOD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479658"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..965)
                     /locus_tag="VF720_03055"
     CDS             complement(168..965)
                     /locus_tag="VF720_03055"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEX7878357.1"
                     /translation="MEGSFTPPTIAGTGQSGFEETPIRNEGGQFPPGQGGPTPPLDGP
                     PPGPPHLPPVAPIPWEEPGRALVNAFVDTITLLYSRCSEAFARMPLNSELLKPIIFAI
                     LVGMIGAVAETVYNSIWSMTIWKLFPWMDEQEHPGFGVFGNFVALFFMPLLIPMFLVI
                     ASAITHIMLILLGAGNRGWTATFRVSCYASASALCQVIPFVGGLASGIVGLIFATHGL
                     AVVHGTSRGRALFAQLLPAAICCGVLSLGLLVFGSAMLAGFKELWNK"
     gene            1054..2376
                     /locus_tag="VF720_03060"
     CDS             1054..2376
                     /locus_tag="VF720_03060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7878358.1"
                     /translation="MPPPLLSELSPVTPRPPLSRVSRYTARFHMPAAFFDGLVAGTFT
                     LNDIVLRKTFGASALLITLMVMAQPVSQLLAIVWGRLMHGRSKRPFILAFGGFGRTLL
                     LLVALASTATAFAVPVVLSIALAIAIIPALNALYQSNYPDTERGRVFGQVLAVTSLAT
                     IVSSMAAGWAMDHDREAYRIIYPLTGLVGLLGIWLYWRIRPRPAKGGAATVVRGGDIS
                     PSEFFDALADAVRNPFRGAKTLYVADPNFLRFEIGYMLYGLGFMMLQPVIPLYLVDEI
                     QVHYSEAATARGLIFWGMMALVSPVFGRLLDRWNAVRLSILGFAVLAVFPILLAVSHS
                     LPGVYGAFALFGFAMSPVNIAWTMGPILFAGKKDAATYMGVHVTMVGIRGLIGNPMGL
                     VLFKTFGSRPTFLVATLFFVAAMVVMIRLNRRLTPTPAPAPVPPPLPA"
     gene            complement(2327..2947)
                     /locus_tag="VF720_03065"
     CDS             complement(2327..2947)
                     /locus_tag="VF720_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878359.1"
                     /translation="MERTAPTRIILTFLLALGLYGFMLPLWLSVESFWARGVAAGASR
                     LAMPTTGQPGSVQLKPGRSIHFEYLLPDPRTGGTARVPQRLLNLPDVPLGIALSLSLL
                     GLAWRRRVVLAAGITAILYLVHVVLLTVSAHRAAALLRQTGLDLTELNRRLSESLNGM
                     NRYGDLSVILVVALAVVGWALLGGRRAVPAIRPEAEAEPAPAPGSE"
     gene            complement(2914..>3381)
                     /locus_tag="VF720_03070"
     CDS             complement(2914..>3381)
                     /locus_tag="VF720_03070"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HEX7878360.1"
                     /translation="LFAFFSWILRVDSVLPIVGGFVRVATEAQASIAAALLRLAGQPV
                     VRKGLLLNGNGFACEVGEGCNGFVALTLAAAAVLAWPVSWRRRLTGLLWMLPLVLALN
                     VLRIAGLWWVGRHRPDWFDLAHVYVGQVVVVVGTVAAWGIWLSWNEPRRPASS"
BASE COUNT          574 a         1167 c         1114 g          526 t
ORIGIN      
        1 acgggcccgc gcttccggcg tgtcggccag ggcgtggggg atgaccttga tggccacgac
       61 gcggtcgagc cgcgtgtcgc gcgcccggta cacctcgccc atcccccccg ccccgatggg
      121 cgactcgatc aggtaatggc cgagccggat gccgggcgcg agtcggctca cttgttccag
      181 agttccttga agccggccag catggccgac ccgaaaacga ggaggccgag cgagaggacg
      241 ccgcagcaga tggccgccgg gaggagctgc gcgaagagcg cccggccgcg cgaggtaccg
      301 tgcaccaccg ccaatccgtg ggtggcgaag atgagcccca cgattccgct ggcaagaccg
      361 ccgacgaacg ggatgacctg gcagagcgcc gacgcggagg cgtagcacga cacgcggaaa
      421 gtggcggtcc agccgcggtt gcccgccccg aggaggatca gcatgatgtg cgtgatcgcc
      481 gaggcgatca ccaggaacat cgggatcagg agcggcatga agaagagtgc cacgaaattg
      541 ccgaagacgc cgaatcccgg gtgctcctgt tcatccatcc acgggaatag cttccagatc
      601 gtcatgctcc agatcgagtt gtagaccgtt tcggccaccg caccgatcat gcccaccagg
      661 atggcgaaga tgatcggctt cagcagctcg gagttgagcg gcatccgggc gaaggcctcg
      721 gaacagcggc tgtaaagaag cgtgatggtg tcgacgaagg cgttgacgag ggcacgcccc
      781 ggctcttccc atggaatggg agcaacaggc gggagatgcg ggggaccggg cggcggtcca
      841 tccaatggcg gcgtcggacc gccctgaccg ggcggaaatt ggcccccctc gttccggatt
      901 ggcgtctctt cgaaaccgga ttggccggtt ccggcgatcg tgggcggcgt gaacgaccct
      961 tccatgcaac cccctcgcgt cgaatatcgc tgactttcga aatcgcgccg cagtatacac
     1021 gcggcgcccc ctgttagaat tcagtggcag accatgccac caccccttct ttccgaactc
     1081 agccccgtca cgccgcgccc ccccttgtcg cgcgtgtcgc gctacacggc ccggtttcac
     1141 atgccggccg ccttcttcga cggcctcgtg gccggtacct tcacgctcaa cgacatcgtg
     1201 ctgcgcaaga cgttcggcgc cagcgccctg ctgatcacgc tgatggtgat ggcacagccc
     1261 gtgtcgcagc tcctggccat cgtgtgggga cggctgatgc acggccgttc gaagcgcccc
     1321 ttcatcctgg cgttcggcgg tttcggccgg acgctcctgc tcctggtggc gctggcctcc
     1381 accgccaccg cattcgccgt gccggtggtc ctgtcgatcg ccctggccat cgccatcatt
     1441 ccggcgctca acgccctcta ccaatccaac tatcccgaca ccgaacgggg acgcgtcttc
     1501 ggccaggtgc tggccgtgac ctcgctggcc acgatcgtgt cgtcgatggc cgcggggtgg
     1561 gccatggacc acgaccgcga ggcgtaccgc atcatctatc cactgacggg gctcgtgggg
     1621 ctgctcggca tctggctcta ctggcggatc cgcccccgcc cggccaaagg gggagcggca
     1681 acggtggtgc ggggcggcga catctccccg tccgagttct tcgatgccct ggccgacgcc
     1741 gtcaggaacc cgttccgcgg cgcgaagacg ctctacgtgg ccgacccgaa cttcctgcgc
     1801 ttcgaaatcg gctacatgct gtacggcctc ggcttcatga tgttgcagcc ggtcatcccg
     1861 ctgtacctgg tggacgagat ccaggtgcac tactcggagg cggccaccgc ccgggggctc
     1921 atcttctggg ggatgatggc gctggtgtcg cccgtgttcg gccggctgct cgaccggtgg
     1981 aacgccgtcc gactgtcgat cctcggcttc gcggtgctgg ccgtctttcc gatccttctg
     2041 gccgtgagcc actccctgcc gggcgtgtac ggggcgttcg cgctcttcgg cttcgcgatg
     2101 tcgccggtca acatcgcctg gacgatggga ccgatcctct tcgccgggaa gaaggacgcc
     2161 gccacgtaca tgggggtgca cgtcaccatg gtcggcatcc gcggcctgat cggcaatccg
     2221 atggggctcg tgctgttcaa gaccttcgga agccggccca ctttcctcgt ggccacgctc
     2281 ttcttcgtgg ccgccatggt ggtgatgatc cggctgaatc gacggcttac tccgaccccg
     2341 gcgccggcgc cggttccgcc tccgcttccg gcctgatcgc ggggaccgcg cggcgcccgc
     2401 caagcagcgc ccacccgacc accgccagcg ccaccaccag gatcaccgag agatcgccgt
     2461 aacggttcat gccgttcagc gattcgctca accggcgatt cagctcggtg aggtcgagcc
     2521 ccgtctgtcg aaggagcgcc gcggcgcggt gcgccgaaac ggtgagcagg accacgtgca
     2581 ccaggtacag gatggcggtg atacccgctg ccagcaccac ccgccgccgc caggccagcc
     2641 cgagcagtga aagcgagagg gcgatgccga gcggcacatc cggaaggttc aggagccgtt
     2701 gaggcacgcg ggccgtgcca cccgtcctcg gatcggggag gagatattcg aagtggatgc
     2761 tgcggccggg cttgagttgc acgcttcccg gttgcccggt cgtcggcatg gccagacggg
     2821 aagcgccggc cgccacgccg cgggcccaga acgattcgac tgacagccag agcgggagca
     2881 tgaacccgta gaggccgagg gccaggagga aggtcaggat gatgcgggtc ggcgcggttc
     2941 gttccacgac agccagatcc cccatgcggc cacggtaccg acgacgacga cgacctgccc
     3001 cacgtagaca tgcgccaggt cgaaccagtc gggacgatga cgccccaccc accacagccc
     3061 cgcgatccgc agcacgttga gcgccaggac cagaggcagc atccagagaa gaccggtgag
     3121 gcgtcgacgc cacgacaccg gccaggccag cacggccgcc gcggccagcg tgagcgccac
     3181 gaatccgttg cacccttcgc ccacttcgca ggcaaagccg ttcccgttca gcaggagccc
     3241 cttgcgaacg accggctgcc cggccagccg caggagcgcc gcggcgatcg aggcctgtgc
     3301 ctcggtggcc acccgcacga atccaccaac gatcggcagg acgctgtcga cccgtaagat
     3361 ccacgagaag aatgcgaaga g
//
LOCUS       DASVOD010000152         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_583780, whole genome shotgun sequence.
ACCESSION   DASVOD010000152 DASVOD010000000
VERSION     DASVOD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583780"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF720_03075"
     CDS             <1..337
                     /locus_tag="VF720_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915521.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEX7878361.1"
                     /translation="VSTGLTSGDSRPVYGRLFQTPFSDRIRNEGKVPTIAVGNITDAE
                     QVNMILAGGRADLVALARPHLADPHWTLRAAADLGWRGVEWPPQYRAARQQLERELAR
                     KAEQRKGSS"
     gene            334..>2148
                     /locus_tag="VF720_03080"
     CDS             334..>2148
                     /locus_tag="VF720_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7878362.1"
                     /translation="MKLGAGSTVGPYQIEAPLGTGGMGAVFRARDTRLGRDVAVKALT
                     DSIGAEPERLGRLRREAQTLAALNHPNIATIYGLEETSDGPCLVLELIDGESLAERLT
                     RGPLPVRDALEIGVQVAAAIETAHERGIIHRDLKPANIMITRSGLAKVLDFGLARNEA
                     GPMPSSDPSSSQALTTEAAATQAGMIVGTIAYMSPEQARGKVLDRRSDVWSFGCVVFE
                     CLTGTRTFAGETASDLIARVLEREPDWSLLPAGTPPRVREILKRCLRKDADARPRDIR
                     DVRLELAEASAGPLRGADTGEKSIAVLPFENLSGPDDEYFADGVTEEILNALTHLEGM
                     RVAARTSCFAFKGRREDLRSVGEKLDVATVLEGSVRRSGPRIRITVQLVNVADGYQLW
                     SERYDREMRDVFELQDEIAAAIAGKLRGTMRDEADRRRASPGTRNLEAFELLLKGRAL
                     QNKRGRFLAEAAACFERAIELDPHYAEAMAWLSDSYRLMGTFGAAPANQVMPRALALA
                     RNALEIDSGQAEAWASMATVEEHWTWDFPLASEHWERALTLDPRHARARTQRALWACI
                     RGDMTDAEAVAESTRAVEDDPLNAWVGAMHSHILGVVGR"
BASE COUNT          340 a          729 c          780 g          299 t
ORIGIN      
        1 cgtgtcgacc ggcctcacgt ccggcgactc gcgcccagtc tacggacgcc ttttccagac
       61 gccgttcagc gaccggatcc gcaacgaagg aaaggtgccg accatcgccg tcggcaacat
      121 caccgacgcg gagcaggtca acatgatcct ggccggtggc cgggccgacc tcgtggcact
      181 ggcgcggccg cacctggccg atccgcattg gacgctgcgc gcggcggcgg acctcggctg
      241 gcgcggcgtg gagtggccgc cccagtaccg cgcggcgcga cagcagctgg aacgcgagct
      301 ggcgcggaag gccgaacagc ggaaggggtc gtcgtgaaac tgggcgccgg ctccacggtc
      361 ggtccgtacc agatcgaggc gccgctcggc acgggtggca tgggcgccgt gttccgcgcg
      421 cgtgacacgc gcctcggccg cgacgtggcg gtgaaggcgc tcaccgactc gatcggggcc
      481 gagccggaac ggctgggccg cctgcggcgg gaggctcaga ccctggccgc cctcaatcac
      541 cccaacatcg ccacgatcta cgggttggaa gaaaccagcg acggcccctg cctcgttctc
      601 gaactcatcg acggggagtc gctcgccgag cgattgacgc gggggccctt gccggtgcgc
      661 gacgcgctgg aaatcggcgt gcaggtggcg gcggccatcg agaccgcgca cgagcgcggc
      721 atcatccacc gcgacttgaa gccggccaac atcatgatca cccggtccgg cctggccaag
      781 gtcctggact tcgggctggc gcgcaacgag gcggggccca tgccgtcgtc ggatccgtcg
      841 tcgtcccagg cgctgacgac ggaagccgcc gccacgcagg ccggcatgat cgtcggcacg
      901 atcgcctaca tgagcccgga acaggcgcgg ggaaaggtgc tggatcggcg gagcgacgtc
      961 tggtcgttcg gctgcgtggt tttcgagtgc ctgaccggta cgcgcacgtt cgccggggaa
     1021 accgcctcgg acctgatcgc ccgcgtgctc gagcgcgagc cggactggtc actcctgccg
     1081 gccggcacgc cgccccgcgt gcgcgagatc ctgaagcgct gcctgcgaaa ggacgccgat
     1141 gcccggccac gggacatccg cgacgtgcga ctcgagctgg ccgaggcgtc ggccggcccg
     1201 ttgcgtgggg cggacaccgg tgagaagtcg attgccgtgc tgccgttcga aaacctgagc
     1261 ggtcccgacg atgaatattt cgccgacggt gtcacggaag agatcctgaa cgcgctcacc
     1321 catctcgagg gaatgcgcgt ggcggcgcgg acgtcgtgct ttgccttcaa aggacgacgc
     1381 gaggacctgc gctcggtggg cgagaaactc gacgtggcca ccgtgctgga ggggagcgtc
     1441 cgtcgatcgg gaccgcggat tcgcatcacc gtccagctcg tcaacgtggc cgacggctac
     1501 cagctctggt cggaacgcta cgaccgggag atgcgcgacg tcttcgagct gcaggacgag
     1561 atcgccgccg cgatcgccgg caagctgcgc ggcacgatgc gcgacgaggc cgatcgccgc
     1621 cgggcgagcc ccggcacgcg caacctggag gccttcgagc tcctcttgaa ggggagggcc
     1681 ctgcagaaca agcgggggcg cttcctcgcg gaagccgcgg cgtgcttcga acgggccatc
     1741 gagctcgatc cgcactatgc cgaggcgatg gcgtggctgt cggactcgta ccggttgatg
     1801 ggcacgttcg gtgccgcacc ggccaaccag gtgatgccgc gggcgctcgc cctggcccgg
     1861 aacgccctgg aaatcgattc cggccaggcc gaggcgtggg cctcgatggc caccgtcgag
     1921 gagcactgga cctgggattt cccgttggcc tcggaacact gggagcgggc gctcacgctc
     1981 gacccgcggc atgcccgcgc ccgcacccag cgcgcgctct gggcctgcat ccggggcgac
     2041 atgacggatg cggaggccgt ggccgagtcg acacgggccg ttgaggatga tcccctgaac
     2101 gcgtgggtcg gagccatgca ctcccacatc ctgggagtag tggggcgg
//
LOCUS       DASVOD010000153         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_583832, whole genome shotgun sequence.
ACCESSION   DASVOD010000153 DASVOD010000000
VERSION     DASVOD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583832"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /locus_tag="VF720_03085"
     CDS             <1..1759
                     /locus_tag="VF720_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878363.1"
                     /translation="MLGPNGYIVHTGLTGDMRFGFYEDQESNGNSDDCAFNTYDHDEL
                     FNDGDAGLLFPSPFSMGPPEYPAGPFGSDHELGTGCSSVHWGTEIDIHYVNNTLHEAY
                     INVIFDFDGNGEWGGQFTCFPGPLLVIEHAVRNVKVSATNNGNLSSVLNALGQAYTAV
                     GGERFVWARFTISEFPVPPDWDGAGIFGDGETEDYLFKIGAPLPAPNGPVTATATVPL
                     TWEPDQSIVINSGPCAAELGDAPDGIRAYSDVSGLPRDGLFPTRRNGIHGSIVHAANA
                     SDENCFFGFEADYEMDATGTCAGGGHETDESSPVDDCDPGIHGPLTYTIDASGLEVPT
                     AFYPGSEPFFGTGSSTVSWGQGPGQFDLYVQNTRGSIAFVNAVIDFDRDGDWDVFTAP
                     QVRGGLDPASPDAGELMATNRAATNIVVPANYVGWLSGLAPPPLNLAGQGGYAWARFS
                     ITSVPISPSWNGSGNFPFGESEDYLFRLNPTASSGVTGPATTAGLQLAQNLPNPMTAS
                     TQIGYRLDQAGEARVDLFDVSGRLVRRLEAGTFEAGDRTVTWDGRDASGGIAPPGIYL
                     YRLSVNGRSLSRKLILSGG"
BASE COUNT          304 a          656 c          602 g          305 t
ORIGIN      
        1 catgctcggt ccgaacggct acatcgtgca caccggcctg accggcgaca tgcgattcgg
       61 cttctacgag gaccaggagt cgaacgggaa ctccgacgac tgcgccttca acacctacga
      121 ccacgatgag ctcttcaacg acggcgacgc gggccttctg ttcccgtcgc cgttcagcat
      181 gggcccgccg gagtatcccg ccggtccctt cggctccgat cacgagctcg ggacggggtg
      241 cagcagcgtg cactggggca ccgagatcga catccactac gtcaacaaca cgctccacga
      301 ggcctacatc aacgtgatct tcgacttcga cggaaacggg gagtggggtg gccagttcac
      361 ctgcttcccc gggcccctcc tcgtcatcga gcatgccgtg cgaaacgtga aggtgtccgc
      421 caccaacaac gggaatctct cgtcggtatt gaacgcgctc gggcaggcgt acacggccgt
      481 cggcggcgaa cggttcgtct gggcccgctt caccatttcc gaatttccgg ttccgcccga
      541 ctgggacggc gccggcatct tcggcgacgg cgagaccgag gactacctct tcaagatcgg
      601 cgcgccgctg ccggcgccca atggcccggt caccgcgacc gcgacggtcc ctctcacctg
      661 ggagcccgac cagtcgatcg tgatcaacag cggtccgtgc gcggccgagc tgggcgacgc
      721 tcccgacggc atccgcgctt actcggacgt gagcggcctc ccgcgggacg gcctgtttcc
      781 gacgcgtcgc aacggcatcc atggctcgat cgtgcacgcg gccaatgcct ccgatgagaa
      841 ttgcttcttc ggcttcgagg ccgactacga gatggacgcc accggcacat gcgcgggagg
      901 cggtcatgaa accgacgaat cctcgcccgt cgatgactgc gatccgggaa tccacgggcc
      961 gctgacctac acgatcgacg cttccggcct cgaagtgccc accgccttct atcccggcag
     1021 cgaaccgttc ttcggcaccg gcagctcgac ggtgtcgtgg ggacagggac cggggcagtt
     1081 cgacctgtac gtccagaaca cccggggcag catcgccttc gtgaatgccg tcatcgattt
     1141 cgaccgcgac ggcgactggg acgtgttcac ggcgccccag gtgcggggcg ggctcgaccc
     1201 ggcttcgccc gacgcggggg aactgatggc gaccaaccgg gccgccacca acatcgtggt
     1261 gccggccaac tacgtgggat ggctgtcggg cctcgcgccg ccgccgctca acctggcggg
     1321 acagggcggg tacgcctggg cgcgcttttc catcaccagc gtgccgatct cgcccagctg
     1381 gaacggcagc ggcaactttc ccttcgggga gtcggaggac tacctcttcc gcctcaaccc
     1441 gacggcgagc tcgggggtga cgggccccgc gaccacggct gggctccagc tggcccagaa
     1501 cctgccgaac ccgatgacgg catccacgca aatcggctac cgcctggacc aggcggggga
     1561 ggcccgagtc gatctcttcg acgtcagcgg tcggctggtc cgccggctcg aggccggcac
     1621 cttcgaggcc ggcgaccgga cggtcacctg ggacggccgg gacgcctccg ggggcatcgc
     1681 gcccccgggc atctacctct accgcctctc cgtgaacggc cgctccctct ccaggaagct
     1741 gattctttcg ggaggatgac acctatgagc accgtttgac gcttaggtct ttggggatgg
     1801 gtccggtcgg ctggacgttc ggcccggctc cgatgacagt ggatcgccgt ccgattgcag
     1861 ggctgcg
//
LOCUS       DASVOD010000154         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_114744, whole genome shotgun sequence.
ACCESSION   DASVOD010000154 DASVOD010000000
VERSION     DASVOD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114744"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF720_03090"
     CDS             <1..275
                     /locus_tag="VF720_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEX7878364.1"
                     /translation="ALTALDNPYDALVGPDGAVWIADTGNNRVRRIAADGTMTTIAGT
                     GVRGYSGDGGPAAEAELDAPSAIAFDASGSLYIADTYNNRIRVIPN"
     gene            272..739
                     /locus_tag="VF720_03095"
     CDS             272..739
                     /locus_tag="VF720_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878365.1"
                     /translation="MSRPPRLRAVIGSLVAAFVIAANIFTGCGGDSTSPGPPPDCTSV
                     ADTSQPATVTYTGQIAALIDHYACATAGCHGSIGTRSRYTVVSYDDLFDPGEQATALR
                     VCAIRPGMPDSSYFYWKLEGHAGIRGVQMPNDRPKLTPEHLAMVRTWIEEGAR"
     gene            complement(804..1952)
                     /locus_tag="VF720_03100"
     CDS             complement(804..1952)
                     /locus_tag="VF720_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878366.1"
                     /translation="MIRTAAWLSAAGVLALLVGGCGGDDSGGGTPVQEDGRLYNVAGI
                     ADEPGGGHDGQVATRSRLYWPQDMTMNAAGELLIADWNNHVIRSVGTDGKIKRIMGSG
                     NHGDDSNGPALQINLNHPDGIAMGPDGNIYVSGWHNWKIKMCDVSTQIVSSVVGNDNG
                     FGGDGGLAINAAISLPSCVVWDQAHNMYVSDQGNNRIRRVDHATAIITTFAGAEKGYA
                     EGTGAEAHFAFPNGTDAYPGGRIDVSADGQYLYVADTENNRVRRIEIATQVVTTIGGT
                     GAATYSGDGGPAVNAGINYPTDIACTPEGDIFVADSKNHVIRKIDTDGVITTIAGTGV
                     YGNSPDGTLATEANLDTPSALYYRAATRTLYIADTYNCQVKKVKLPVE"
     gene            complement(2038..3231)
                     /locus_tag="VF720_03105"
     CDS             complement(2038..3231)
                     /locus_tag="VF720_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878367.1"
                     /translation="MIYWRLNVLSRRTLSALVALPVLVLTGCGGDDDGGVEPGLEKIS
                     YVVAGVAGESGHVGDDGPATDALLYWPIDMTVVPNGQLVIMDWNNHCARKIDADGNIH
                     AFIGSGRLGDDRTGAMTAIDFNHPSDFKIGPDGSYYVAAFHNWAIRKIDPVTGQATTP
                     VGKVAASGVGSVRGHEGDGGPANVASLNLPGSIDFDANGNLYITDQGNMRIRMVDPNG
                     VITLIAGGENGYADGPALEARFNFEGEQATTGTGTRSGCIDISKDGRYLYIADTLNNR
                     VRRLEFATGMVTTVAGTGAVGYSGDDGPALAATFNHPSDVVCTVSGDVYVSDRYNNVI
                     RKIDPAGTITTVVGTGQAGFTPNGTPARETKLYNPLGLDFDDANNTLYIADMYNHQVL
                     KVINP"
     gene            3447..4151
                     /locus_tag="VF720_03110"
     CDS             3447..4151
                     /locus_tag="VF720_03110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7878368.1"
                     /translation="MTFKDKRALITGGSDGIGLATGKLLREKGCRVALMARNPEKLAA
                     AAAEIGALSIPGDVARAEDARGAVERTVAEFGGIDILVNNAGIGTWGPLVELDIAEFD
                     RVFAVNVRGALLVTQAAAPHMIRHGSGNILNIASTSGLKGSANGTIYCSSKFALRGMT
                     ECWRDELRRHNIRVTLVNPSEVQTNFFVANSLAPRPESPKKLRSQEIAAAIVGALEID
                     DRGFIPEFSVFATNPF"
     gene            4222..4485
                     /locus_tag="VF720_03115"
     CDS             4222..4485
                     /locus_tag="VF720_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878369.1"
                     /translation="MRSASLRPLSLILTLLFIWAMMAAVSGCESIPVKRHDRVLTQAE
                     HDSILATETAIPGTVVVARALDAKERNAQKTAALNAQIDSLTR"
     gene            4524..>5293
                     /locus_tag="VF720_03120"
     CDS             4524..>5293
                     /locus_tag="VF720_03120"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7878370.1"
                     /translation="MSRHPIQYREFVPHLRLRPFVECYWRVWGTVEASPKPERVVPDA
                     CPEIIFHCANPFRRQIGARWIVQRSTFLAGTLSAPWRLKPGRRVDTVGVRFKPGMITA
                     MFPVVMSEMADRETELESFVPAVDVATLLAQLRNATPARRVHLVDDWLLPRLEHRAPG
                     GHSVTRTAVALLLQSRGRRRVDDVASRLAVPRRRLERLFARDLGIRPKLFARIARLNH
                     ALARLEREERDGAVDAALAAGYFDQSHMSREFRNVAGRR"
BASE COUNT          853 a         1791 c         1736 g          913 t
ORIGIN      
        1 ccgcgttgac ggcgctcgac aacccctacg acgcgctcgt cgggcccgac ggcgccgtct
       61 ggattgccga caccggcaac aaccgggtgc gtcggatcgc ggccgacgga acgatgacca
      121 ccatcgccgg caccggcgtg cgcggctact cgggcgacgg ggggccggcc gcggaagccg
      181 agctcgacgc cccctcggcc atcgccttcg acgcaagcgg cagcctgtac atcgccgaca
      241 cgtacaacaa ccgaatccgc gtcatcccga attgagccgc ccgccccgac tccgggcggt
      301 catcggctcc ctggtcgccg cctttgtcat cgccgccaac attttcaccg gctgcggcgg
      361 cgactccacc agccccggcc caccgcccga ctgcacgagc gtggccgaca ccagccagcc
      421 cgccaccgtc acctacaccg gccagatcgc cgcgctcatc gaccactacg cctgcgccac
      481 tgccggctgc cacggctcga tcggcacccg ttcgcgctac acggtcgtca gctacgacga
      541 tctcttcgac ccgggtgaac aggccaccgc gctccgcgtc tgcgcgattc gaccgggcat
      601 gccggactcg agctacttct actggaagct cgaagggcac gcgggcatcc gcggcgtgca
      661 gatgccgaat gatcggccga agctcacccc tgagcacctg gcgatggtgc ggacgtggat
      721 cgaagagggg gcgcggtaaa gcaagaggcc ggcgcccctg aggatgccgg cctttgagat
      781 ttccgatgct gtcgaaccgc cgatcactcc accggcagct tcaccttctt gacctggcag
      841 ttgtaggtgt cggcgatgta gagcgtgcgc gtggccgcgc ggtagtacag ggccgagggc
      901 gtatccaggt tggcctcggt cgcgagcgtg ccgtccgggg agttgccgta gacgcccgtg
      961 ccggcgatgg tggtgatgac gccgtccgta tcgatcttcc ggatgacgtg gttcttggag
     1021 tcggccacga agatgtcccc ctccggcgtg caggcgatgt cggtcggata gttgatgccg
     1081 gcgttcacgg ccgggccacc gtcacccgaa taggtggcgg cgcccgtgcc gccgatcgtc
     1141 gtgacgacct gcgtggcgat ctcgatgcga cggacccggt tgttctcggt gtcggccacg
     1201 tagagatact gtccgtcggc gctcacgtcg atgcggccac ccggataggc gtccgtgccg
     1261 ttcgggaagg cgaagtgggc ttcggcgccc gtcccttcgg catatccctt ttcggcgccc
     1321 gcaaaggtcg tgatgatggc cgtggcgtga tcgacccggc ggatccggtt gttcccctgg
     1381 tcggacacgt acatgttgtg cgcctggtcc cacaccacgc acgacggcag ggaaatggcc
     1441 gcattgatgg ccaggccgcc gtcaccgccg aagccgttgt cgttaccgac cacggacgag
     1501 acgatctggg tggagacatc gcacatcttg atcttccagt tgtgccagcc cgacacatag
     1561 atattcccgt cgggccccat cgcgatgccg tcgggatggt tcaggttgat ctggagggcc
     1621 gggccgttcg agtcgtcgcc gtggttgccc gagcccatga tccgcttgat cttgccgtcg
     1681 gtgccgaccg aacggatgac atgattgttc cagtcggcga tgagcagctc gcccgccgcg
     1741 ttcatggtca tgtcctgggg ccagtacagt cgcgagcgcg tggccacctg gccatcgtgt
     1801 ccgccgcccg gctcgtcggc gatgccggcc acgttgtaga ggcggccgtc ttcctggacc
     1861 ggtgtgccgc cgcccgagtc gtcgccgccg cagccgccca cgaggagcgc cagcacgccc
     1921 gcggcggaga gccatgccgc ggtgcgaatc atggtccgat gtcctggttg caatgtcgtc
     1981 atatgttcga acctccggaa cggcgcccgt gccgcatgcg gcgggcgcgg tcgtcgtcta
     2041 cgggttgatg accttcagca cctgatggtt gtacatgtcg gcaatgtaga gcgtgttgtt
     2101 ggcatcgtcg aagtcgaggc cgagcgggtt gtagagcttg gtttcccgcg ccggcgtgcc
     2161 gttcggcgta aaccccgcct ggccggtgcc gaccaccgtg gtgatcgtcc cggccgggtc
     2221 gatcttccgg atgacgttgt tgtagcgatc ggaaacgtag acatccccgc tcaccgtgca
     2281 gaccacgtcc gacgggtgat tgaacgtggc ggcaagcgcc ggcccatcat cgcccgaata
     2341 gcccactgcg cccgtgccgg ccacggtcgt caccatgccg gtggcaaact cgaggcgacg
     2401 cacccggttg ttcagtgtgt cggcaatgta caggtaacgg ccatccttcg agatgtcgat
     2461 gcagccgctc ctcgttcccg tgccggtggt ggcctgctca ccctcgaagt tgaatctggc
     2521 ttccagggcc gggccatccg cgtagccgtt ctcgccgccg gcgatcaagg tgatcacgcc
     2581 gttcggatcg accatgcgga tccgcatgtt cccctggtcg gtgatgtaga ggttgccgtt
     2641 ggcgtcgaag tcgatcgagc cggggaggtt cagcgacgcc acgttggccg gaccgccgtc
     2701 tccctcgtgg ccacgcaccg agccgacccc gctggccgcc accttgccga ccggcgtggt
     2761 ggcctgtccc gtgacgggat cgatcttgcg gatggcccag ttgtggaacg cagccacgta
     2821 atacgagccg tcgggtccga tcttgaagtc gctcggatga ttgaaatcga tggcagtcat
     2881 ggcgccggtg cgatcgtcgc ccagtcggcc cgagccgatg aacgcgtgga tgttcccgtc
     2941 ggcgtcgatc ttccgggcgc agtgattgtt ccagtccatg atgaccagct ggccgttcgg
     3001 caccaccgtc atgtcgatgg gccagtaaag gagcgcgtcg gtggccggac catcgtcacc
     3061 gacatggccg gattcgccgg ccacgcccgc cacgacgtag gagatttttt ccaggccggg
     3121 ctccacgccc ccgtcgtcat cgccaccgca accggtgagc acgaggacgg ggagggcgac
     3181 gagagcggac agtgtgcgcc gagaaagcac attcaggcgc caatagatca tgaattcctc
     3241 catatgggga taattcgacg acgcgatgtc atgcagggtg gccggcacgg acgctgccgc
     3301 cgccgggtca agcctagact atagtgtgaa ccggccatcg ttcacaagga aatcccggca
     3361 gcatgacacc gggctaagcc ccagtcagac accagtttgg caatcttttc ggcaactttt
     3421 ccgacaccca gaaacgaggg aagtccatga cattcaagga caagcgcgcc ctgatcaccg
     3481 ggggtagtga tggtatcggc ctggccacgg gcaagctgtt gcgggaaaag gggtgccggg
     3541 tggcgctcat ggcccggaac cccgaaaagc tggctgccgc cgcggcggag atcggggccc
     3601 tgtccatccc gggggacgtg gcgcgcgcgg aggacgcccg gggagccgtc gagcgcacgg
     3661 tggccgaatt cggcggcatc gacatcctgg tcaacaacgc cggcatcggg acctggggcc
     3721 cccttgtgga gctggacatc gccgagttcg atcgtgtctt tgccgtgaac gtgcggggag
     3781 ccctcctcgt aacccaggcg gccgcccccc acatgatccg ccacgggtcc ggcaacatcc
     3841 tcaacatcgc ctccacctcc gggctcaagg gctccgccaa cggcacgatc tactgctcga
     3901 gcaagttcgc gctccgcggc atgaccgagt gctggcgcga cgagctccgc cgccacaaca
     3961 tccgcgtgac gctcgtgaac ccgagcgagg tgcagaccaa tttcttcgtg gccaactccc
     4021 ttgcgccccg cccggaaagc ccgaaaaaac tgcgaagcca ggagatcgcc gccgccatcg
     4081 tcggggcatt ggagatcgac gaccgggggt tcatcccgga gttctcggtg ttcgcgacga
     4141 acccgttcta ggaacgggcc agatgcccca ccggcccggt ccggcatcaa gccgatggcc
     4201 gggccggacg ataacgggat catgagaagc gcctccctgc ggccgctgtc gctgatcctc
     4261 acgctcctct tcatctgggc catgatggct gccgtctccg gctgcgagag catccccgtc
     4321 aagcggcacg accgcgtcct gacgcaggcc gaacacgaca gcatcctggc caccgagacc
     4381 gccatccccg gcacggtcgt ggtggcccgg gcgctcgacg cgaaggaacg gaacgcccag
     4441 aagaccgcgg cgctcaacgc ccagatagac tcgctgacgc gctgacaccg gtcggtcctc
     4501 ccgcgccgag ctagaatgcg gcgatgagtc ggcaccccat ccagtatcgc gagtttgtcc
     4561 cccatctccg gcttcgcccc ttcgtcgagt gttactggcg ggtgtggggc acggtggagg
     4621 catcgccgaa gccggaacgc gtcgtaccgg atgcgtgtcc ggagatcatt ttccactgcg
     4681 cgaatccatt ccgccggcag atcggtgcgc gctggatcgt ccaacgctcg actttcctgg
     4741 cgggtacgct ctcggcaccg tggcgcctca agcccgggcg acgcgtggat acggtcggcg
     4801 ttcggttcaa gcccggaatg atcacggcga tgtttcccgt cgtgatgtcg gaaatggccg
     4861 accgggaaac ggaactcgaa tcgttcgttc ccgcggtcga tgtcgcgacg cttttggcgc
     4921 agttgcgcaa cgccacgccg gcccggcgtg tccatcttgt cgatgactgg ctgctgccgc
     4981 ggctggaaca ccgggcgcca ggagggcact cggtgacacg cacggccgtc gccctcctgc
     5041 tgcagtcgcg cggtcgccgc cgcgtcgacg atgtcgcttc ccgcctcgcg gtgccccgcc
     5101 ggcggcttga gcgcctcttt gcgcgggacc tcggtatccg gcccaagctc ttcgcccgga
     5161 tcgcgcggct caatcatgcg ctggcccgcc tggagcggga ggaacgcgac ggcgccgtcg
     5221 acgcggcgct cgcggccgga tacttcgatc agtcccacat gtcgcgcgag ttccggaacg
     5281 tggcggggcg acg
//
LOCUS       DASVOD010000155         5020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_218698, whole genome shotgun sequence.
ACCESSION   DASVOD010000155 DASVOD010000000
VERSION     DASVOD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218698"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF720_03125"
     CDS             <1..275
                     /locus_tag="VF720_03125"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011388024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX7878371.1"
                     /translation="LARVVEAYARRFQVQEKMTAQIAHTICDVLQPRGVAVVIEAAHL
                     CMTMRGVEKQNSVAVTSAMLGGFRTDRSSRMEFLNLIRGQARPGGS"
     gene            275..1003
                     /locus_tag="VF720_03130"
     CDS             275..1003
                     /locus_tag="VF720_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7878372.1"
                     /translation="MTPVAPLAGRVALVTGGSRGLGRGVALALATRGAAVVPVSRDAT
                     GLVGTLEELRAMGARTTGRPIAVDVRNLTDLEAAVRDAGSLGTVDILVAAAGVAHIAP
                     IVELSADAWNEQIAVNLTGAFNAVKAVLPGMIAAGRGDVVFLGSVAARRPFTRGSAYG
                     ASKAGLAMLADILRAETRDAGIRVLHVVAGATDTDIWGAEPPAPPERMMSVALVATAI
                     ADAVTTDRRGTMEEIVLRPLLGDL"
     gene            complement(1010..1300)
                     /locus_tag="VF720_03135"
     CDS             complement(1010..1300)
                     /locus_tag="VF720_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX7878373.1"
                     /translation="MTSDDRIEPIGSDPPVDGVPDIIVDAMGLLCPLPLVKVARAIGA
                     LAPGTLLELQCDDPAALADVPAWCEARRHDYLGVCREGSVLRFFFRKGGAAD"
     gene            1532..1996
                     /locus_tag="VF720_03140"
     CDS             1532..1996
                     /locus_tag="VF720_03140"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX7878374.1"
                     /translation="MEIIRVLFVCEGNDCVSPMAEGFLKRHGGVAFDVVSAGIHPKPL
                     DATAVDVMHRSGIDISAHPPTAVAELADRDFDFVINVSEASRDAVIGVTNTHRLIHWR
                     FPDPRDGGGTDSEVRRRYQRVRDEVAGRVRLFAYAQTRRRERGTRDSFSLAG"
     gene            2091..3107
                     /locus_tag="VF720_03145"
     CDS             2091..3107
                     /locus_tag="VF720_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7878375.1"
                     /translation="MTRNLMIPNLLAAFVFFLLALTNPTSGADSRPIRVAHLGTLAHA
                     PVLIARERQTFEKAVAPRPVEWKMFNAGPQLIEALFAREVDLAWVGPGPAVNGFARSN
                     GEALRIISGLTQGGAGLVVRKGSGITDAAVLGGKRIATPQAGNTQDLALRHWLDTHAL
                     KPREKGGTVDILPMPAADQLTLFQRGEIDGAWAVEPWVTRLILEGPGELVLDEATIWP
                     DGKYATTVLIARPELLRDEPALIATWLATQESLLTWMNANPEAARDEANAALAKLTGK
                     GLPPAALASAWTRFTFTSDPARNSIETGARQAEALGFLGRRRVELAPIFALDPLAAGR
                     RPQP"
     gene            3104..3871
                     /locus_tag="VF720_03150"
     CDS             3104..3871
                     /locus_tag="VF720_03150"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020247489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878376.1"
                     /translation="MSIVLTGVERGFTVAGRPLPALGPIDLTIASGEFVCIVGPSGCG
                     KTSLLNLVAGLDIPDRGSVVADDERVIGTSADRLLVFQDGGLFPWLNVRENVEFGLRV
                     AGVGARERRERAEQLLAQVHLKPFAEARIHQLSGGMRQRVALARALAPKPKLLLMDEP
                     FAALDAQTRDRLGFELQEIQAATRTTILFITHNLREAGCLGDRVLLMSGRPGRIVREL
                     RVELPRPRRIEDDGLIEAIRPLMADLRREVDQMVAAS"
     gene            3910..4650
                     /locus_tag="VF720_03155"
     CDS             3910..4650
                     /locus_tag="VF720_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014392906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878377.1"
                     /translation="MLVLLLLIWVVLARWGPWPPYLFPGPLDVGQALLAGLGDGSIGS
                     SVLVSLKRLLVGFAISVVAGIVIGLLNARFTVMRETLGLLVLGLQSLPSICWLPLALL
                     WFGLNESAVIFVVVMGSLLSVALAVEDAVRNIPAIYLKAARNMGASGIMLYRTVVLPA
                     SLPTVVSGLKQGWSFAWRALMAGELLFVGPGLGRLLMLGRDLNDMAQVIAVMLVIVAI
                     GLGFDRLVFSPIERRLANAWGPTGIASG"
     gene            complement(4655..>5020)
                     /locus_tag="VF720_03160"
     CDS             complement(4655..>5020)
                     /locus_tag="VF720_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878378.1"
                     /translation="RPAKGGVRTKSFSDGFAFEISIEIDSPLDRVWESWHAATSRRLW
                     LHVAGVSTRKVNAKSSLRLLWNDGSEVKATFSSRAKGRTGVDVRHDRLKSAADQAAIR
                     TLWETSLRRLKTRLERRRG"
BASE COUNT          765 a         1734 c         1709 g          812 t
ORIGIN      
        1 agctggcgcg ggtggtggag gcttatgccc gccgcttcca ggtgcaggaa aagatgaccg
       61 cgcagatcgc ccacaccatc tgcgacgtgc tgcagccgcg cggcgtggcc gtggtgatcg
      121 aggccgccca cctctgcatg acgatgcgcg gggtggaaaa gcagaacagc gtggcggtga
      181 ccagcgcgat gctcgggggc ttccgcaccg atcgctcgag ccggatggaa ttcctgaacc
      241 tgatccgcgg ccaggcccgc cccggcggca gttgatgaca ccggtcgcgc cgctggccgg
      301 acgggtggcg ctcgtcacgg gtggcagccg cggactcggg cgcggcgtgg cattggctct
      361 ggcgacgcgc ggcgcggcag tggtgccggt gtcgcgcgat gcgacggggc tcgtcggaac
      421 gctcgaggag ttgcgtgcca tgggggcgcg cacgacagga cgtccgatcg ccgtcgacgt
      481 gcggaatctg accgatctcg aggcggccgt ccgcgacgcc ggctcccttg gcactgtcga
      541 tatcctggtg gccgctgccg gcgtggcgca catcgcgccg atcgtcgagt tgagcgcgga
      601 cgcttggaac gagcagatcg ccgtgaacct gaccggcgcg ttcaatgcgg tgaaagccgt
      661 cctcccggga atgatcgccg cgggtcgggg cgacgtcgtt ttcctggggt cggtagccgc
      721 ccgacgcccc tttacgcggg ggagcgctta cggcgcctcg aaggcgggcc tcgccatgct
      781 ggccgacatc ctccgcgccg agacccgcga cgccggcatc cgcgtgctcc acgtggtggc
      841 cggggccacc gacaccgaca tctggggagc tgagccgccg gcgccccccg aacggatgat
      901 gagcgtcgcg ctcgtggcga cggccatcgc cgacgcggtg acgaccgatc gccgcgggac
      961 catggaggag atcgtcctcc gccccctcct cggcgacctg tagcgggcct cagtcggccg
     1021 cgcctccttt ccggaagaag aaccggagca cgctcccctc acggcacaca cccaggtaat
     1081 cgtggcgccg tgcttcgcac caggccggaa cgtcggccag agcggcggga tcgtcacact
     1141 gcaattcgag cagcgtaccc ggcgcgagcg caccgatcgc acgggcgact ttcaccagtg
     1201 gcagcgggca gaggaggccc atggcatcca cgatgatgtc gggcacgcca tcgaccggtg
     1261 gatcactccc gatcggctcg atgcggtcgt cgcttgtcaa gtcgtggcct ttcgcacccg
     1321 gcagcccccc ggaggacgcc ggtggaaaaa aagtcacaat ccgcgaatcg cttgcggcgg
     1381 ttcacgatcg gtcgccggtc atcgtaccgc aaccggaatg gggcttcctc ggattgacac
     1441 ctcgggaacc acctcctagc gtttccactg acgaccgtaa cgccgcgtgg tgaccacagc
     1501 gccgcgactg tggaatgtat ccgggggaac gatggagatc atccgagtcc ttttcgtctg
     1561 cgaaggaaac gattgcgtga gcccgatggc cgagggcttc ctgaagcgtc acggcggggt
     1621 cgcgttcgac gtagtcagtg ccggcatcca tccgaagccg ctcgatgcga ccgcggtcga
     1681 cgtcatgcac cgctcgggta tcgacatctc cgcgcacccg cccacggccg ttgccgaact
     1741 cgccgaccgc gacttcgact tcgtcatcaa cgtctcggaa gcgtcgcgtg acgcggtgat
     1801 cggcgtcacc aacacgcacc gcctgatcca ctggcgcttt cccgacccgc gcgacggcgg
     1861 gggaacggac tccgaagtcc gccgtcgtta ccagagggtg cgcgacgagg tggccggccg
     1921 cgtccggctg ttcgcctacg cgcagacccg ccgccgcgag cggggaacgc gggactcctt
     1981 ctccctggcg ggataggcgc tccgaccgaa aaaaacattt gccggacggg gcaaaccgcc
     2041 tgctacaatc tctactagtc ctatagacat taacgactat cgaggcgccc atgacccgca
     2101 acctgatgat tcccaacctg ttggctgcat tcgtcttctt ccttcttgcc ctgaccaacc
     2161 ccacctccgg ggccgattcc cgcccgattc gtgtggcaca cctcggtacc ctggcccacg
     2221 cgccggtcct cattgcccgg gaacggcaga ccttcgagaa ggccgtggcc ccccgccctg
     2281 tcgaatggaa gatgttcaac gccgggcccc agctcatcga agccctcttc gcccgcgagg
     2341 tcgacctggc gtgggtcggg ccggggccgg cggtgaatgg cttcgcgcgc tcgaacggcg
     2401 aggcgctccg gatcatttcg ggccttacgc aaggcggggc ggggctcgtc gtccggaagg
     2461 ggagcggcat caccgatgcc gcggtgctcg gcgggaaaag aattgccacg ccgcaggccg
     2521 gcaacacgca ggacctggcg ctccgtcact ggctcgacac gcacgcactg aagccgcggg
     2581 aaaagggggg gacggtcgac atccttccga tgccggccgc cgaccagctc accctgttcc
     2641 agcgcgggga gatcgacggc gcctgggcgg tggagccgtg ggtgacgcgg ctgatcctcg
     2701 aggggccggg ggaactcgtc ctcgacgaag cgacgatctg gccggatgga aagtacgcca
     2761 ccaccgtctt gatcgcgcgg cccgagctgc tgcgggacga accggcactc atcgcgacgt
     2821 ggctggccac gcaggagagt ctattgacgt ggatgaacgc caacccggaa gccgcgcgcg
     2881 acgaggccaa cgccgcactg gcgaaactga ccggcaaggg attgcccccg gccgcgctcg
     2941 cctcggcctg gacgcgcttc accttcacct ccgatccggc gcgcaactcg atcgaaacag
     3001 gggcaaggca agccgaggcg ctcggcttcc tcggaaggcg ccgggtggag ctggcgccga
     3061 tcttcgcgct cgacccgctc gcggccggcc ggcgaccgca gccgtgagca tcgtcctcac
     3121 cggggtggag cgcggcttca cggtggcggg ccgcccgttg ccggcgctcg ggccgatcga
     3181 cctgaccatc gcctcggggg agttcgtctg catcgtggga ccgtccggtt gcggcaagac
     3241 atcactcctg aacctggtgg ccgggctgga cattcccgac cgggggagcg tggtggcgga
     3301 cgacgaacgc gtcatcggca cgtcggccga ccggctgttg gtcttccagg acggcggcct
     3361 cttcccgtgg ctcaatgtcc gcgagaacgt ggagttcggc ctgcgggtgg ccggcgtcgg
     3421 cgccagggag cggcgcgaac gggccgagca gctgctggcc caggttcacc tgaagccgtt
     3481 cgccgaggcg cgcatccatc agctctcggg cggcatgcgc cagcgcgtgg cactggcccg
     3541 cgcgctggca ccgaagccga aacttctcct gatggacgaa cccttcgctg cgctcgatgc
     3601 ccagacgcgc gaccgcctgg gcttcgagct ccaggagatt caggcggcga cccggacgac
     3661 catcctcttc atcacccata acctgcgcga ggccggctgc ctcggtgacc gcgtcctcct
     3721 catgtccggc cggcccggtc gcatcgtccg ggagctgcgc gtggagttgc cccggccgcg
     3781 ccgcatcgag gatgatggcc tcatcgaggc catccgtccg ctgatggccg acctgcgccg
     3841 ggaggtcgat caaatggtgg ccgcgtcctg agtcgcgaat ggcttcgccg ccaggggcgg
     3901 cgtgcggtca tgctcgtgct gctgctcctc atctgggtcg tcctggcccg atggggtccg
     3961 tggccgcctt acctgttccc cggacctctc gacgtcggac aggcactgct cgcgggactt
     4021 ggcgacggtt cgatcgggag cagcgtcctc gtcagcctca agcgcctgct ggtcggcttc
     4081 gccatctcgg tcgtggccgg catcgtcatc ggcctcctga atgcccgctt cacggtgatg
     4141 cgggagacgc tcggcctcct cgtcctcggc ctgcagtcgc tccccagcat ctgctggctg
     4201 ccgctggcgc tcctctggtt cggcctcaac gagtcggcgg tgatcttcgt ggtcgtgatg
     4261 ggctcgctcc tgtcggtggc gctcgccgtc gaggacgccg tccggaacat cccggccatc
     4321 tacctgaagg cggcccgcaa catgggcgcc tccggcatca tgctgtaccg caccgtggtg
     4381 ctgcccgcgt cgctcccgac ggtggtcagc ggcctgaagc agggatggtc gttcgcctgg
     4441 cgcgctttga tggccggcga acttctcttc gtcgggcccg gcctcggccg actcctgatg
     4501 ctcggccgcg acctgaacga catggcgcag gtcatcgccg tcatgctggt gatcgtggcc
     4561 atcggcctcg gcttcgaccg tctggtgttc agcccgatcg agcggcgcct ggccaatgcg
     4621 tggggaccga cgggaatcgc gtcgggctag aaaatcaacc ccgacgacgt tcgaggcggg
     4681 tcttcagccg ccgaagcgac gtctcccaca acgtgcggat cgccgcctga tcggcggcgg
     4741 acttgagtcg atcgtggcgc acgtccacgc ccgtacgtcc cttggcgcgt gacgagaagg
     4801 tagccttcac ctcggacccg tcattccaga ggagtcggag cgatgacttc gcgttgactt
     4861 tccgggtgga aacccccgcg acgtgcagcc agagccgccg cgacgtggcg gcgtgccagg
     4921 actcccacac gcggtcgagc ggcgaatcga tctcgatcga gatctcgaag gcgaagccat
     4981 ccgaaaagga ctttgtccgc acgccgccct ttgcgggcct
//
LOCUS       DASVOD010000156         6513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_114758, whole genome shotgun sequence.
ACCESSION   DASVOD010000156 DASVOD010000000
VERSION     DASVOD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114758"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /gene="tssI"
                     /locus_tag="VF720_03165"
     CDS             <1..1524
                     /gene="tssI"
                     /locus_tag="VF720_03165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HEX7878379.1"
                     /translation="IRLEFQVKGLDPATFTPVKFEGVEAISELYRYEIDLVAPDTTEV
                     DPDKVVGQPGVLTIDRAGDVTRFHGIVAVFELLGRGNNDATRFRVVMVPRLANLGLTK
                     TSQVFLDKPHWKIVQAVLEENGLADGKDFDVQISEPGPTRDYVVQYQESDLDFIRRLM
                     EYEGIFFFFRHDEEQEVMVLGSAKERHKPIEGEATVTFRDQRDVGPGGEAVGEFIYRQ
                     TQLSKDVFLKDYNYRKPTLEIKGTADVIPKGFGTFMEYGDHFKDPEDGKRLAAIRAQE
                     QSCRQKEWFGRGAMRHFRAGSTFTLQDHFVTKFDGKYLITSVTHSAHQAGAVGGDTGP
                     QGQRPGYLNHFVAIPADTLFIPRRITPRPRIWGYMKAQIDAGGSGDYAEIDDLGRYKV
                     KLPFDLSDAKEGKASHFIRMAQPYSGGGMGMHFPLHKGTEVLLIHLDGDPDRPIIVGS
                     VPNPDTMSPVTGANQTQCVIHTGGGNKIVIEDTDGGQRIAFSSPHSETFFSLGAAPE"
     gene            1528..2361
                     /locus_tag="VF720_03170"
     CDS             1528..2361
                     /locus_tag="VF720_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878380.1"
                     /translation="MGGGPGGAGISAGDPHGAAGDGRGSATTDKNGQVSKTAGDHRED
                     SHNQVSQTQGDSWIEVKGNKNEFKMGAFLELIGGMKHTMVALFKTGLDFSYNRTNDIG
                     PYTTEMFAAKYERLGSKNEKVMGKKTETWTGYKYEKTTGPWVEANAKKRQEAEAKKKA
                     INEALHQRAGAMLEEKATKCKQRAQVVKESVAMLDRRVSKEMRLTCEKAREQVNKHER
                     EISKIEMVTDKFEQKLKAMDASIGSLMSIDAKKITHKAGKYDAKASVLKFTSDLIKLG
                     E"
     gene            2387..3211
                     /locus_tag="VF720_03175"
     CDS             2387..3211
                     /locus_tag="VF720_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878381.1"
                     /translation="MTDGPAPSAGEPTHKDTDPVPPVSSGAFWKTIKDKYDRHENHHG
                     VFDSHSWKLIVGLSVSAVLSKAHKITLGWEHKSVKGGSVTVVMPYDNKLNVGAVCNYI
                     IMDKSETTLGNKSEWILGFKFAKVNGKKQKFEGANKLKVSEIEQAKKTVDLQLHVMAL
                     EKLMAAELERDIKNLEEEHSKAEEIMKKAEATYTQMEVQMKEWTFKSNYLKSDIPKAQ
                     WECDSAEAKYSATWKSLSDAAHEITADGGVDMVAGAEAKFIGKSLIQLGASLTNAG"
     gene            3237..4718
                     /locus_tag="VF720_03180"
     CDS             3237..4718
                     /locus_tag="VF720_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878382.1"
                     /translation="MTVTQDQVDEAKAASEAARKESEAAHAAAEKAKTDAAKARTDAT
                     NARTESNRLKGEVGPLETKAGQLEGEAAASKTACDNAKAAATKAESDATAEEGRAARV
                     QALNPGECNADTVAADGKAKAARDRATALRADAGTKCAKAAEDAKKAEAARAEATAAR
                     TKADGASDKADDLEDAADDAEAAIPGAAATAISMATAAAVAKSKLESLEKELAAQAAN
                     PGTVGPRAPGLNDVAPIVGAPPPTINNPGIHNDTKKNATIGAKMGIFLVGGDSYEKVE
                     GKLIEIIDGSKITVVLGGDFSLVLGAKLEEIVGLESKLVLGVDRRLVVGFRWERNIGI
                     KAETVVGDATEVTLARHDQFIPTLTTGVNPSTRKETTPEMETKAAKVMEEYKARLVDL
                     RKKVKDDFDEAQRHYREVSSHVDKMAEEVKRLEEVIKNLDLKVKDMKTQAKKYDEKAA
                     TFIKIIVSGTAKLKPKRFKGNAEQKFKMVASAIAKLQGEVKWN"
     gene            4726..>6513
                     /locus_tag="VF720_03185"
     CDS             4726..>6513
                     /locus_tag="VF720_03185"
                     /inference="COORDINATES: protein motif:HMM:NF021456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2169 domain-containing protein"
                     /protein_id="HEX7878383.1"
                     /translation="MPTLINDSGLRFGVFVGRVPPAALTATLVVKGAFALRPNGPAEV
                     IPEEEQPHLDGDKAWDDDPARGLRSASDFAPFKPGTDLLLAGHAHAPGGRPVPFTQVS
                     FGVANFRKPAVVIGDRRLKSAMGGSSAGEPEPFTSMPLDWSRTWGGSDVPENPVGRGR
                     HELTRPDGSKVRLAANIESVDQATSASGVFGRRMGFGPIDPNWAERMSRVGTYDGKWL
                     AERWPWYPDDFDWRFFSAAPPDQYLHQIYLKGDEALEFVHLHPEHAVLASRLPGLYAR
                     CFIRVRKGVDLEFREVPMRIDTLFADLDASQVILTWRGVTPAATLKLKEFEDYFVILE
                     PLAAPSGRSLDEYRERHERRLREIAAEFEVEPVILEPIVMPTLTPAATAWAERLEKAA
                     KKLRDAAQVEAQAPPPATGPLGTPMAPLKMPPPPKVNSIADATALAAADFAAIDGHAP
                     GFSAKYPPPDFSEFAADTALFAAFNGLPDMEMDDAEDDPWTRKSVIEHLATGGRFDGE
                     DLSELDLSGLDLSGAGFRGATLEATNLAGAGLIGADFRKASMAGAILDGARCERADFG
                     GADMAEVSGAAAVFAGIQGEASDWTEARLA"
BASE COUNT         1354 a         2063 c         2192 g          904 t
ORIGIN      
        1 atccggctgg agttccaggt gaaggggctc gatccggcca cgttcacgcc ggtcaagttc
       61 gagggcgtgg aggcgatcag cgaactgtat cgctatgaga ttgacctggt ggcgccggat
      121 acgacggagg tcgacccgga caaggtggtg gggcagccgg gcgtgctcac gatcgaccgc
      181 gcgggagacg tcacccggtt ccatggcatc gtggcggtgt tcgagctcct cggccgcggc
      241 aacaacgatg ccacccggtt ccgcgtggtg atggtgccgc gcctggccaa cctcgggctc
      301 acgaagacga gccaggtgtt cctcgacaaa ccgcattgga agatcgtgca ggcggtgctc
      361 gaggagaacg gcctggccga cggcaaggac ttcgacgtcc agatcagcga gcccggcccc
      421 acgcgcgatt acgtggtgca gtaccaggag agcgatctcg atttcatccg tcgcctcatg
      481 gagtacgagg gaattttctt cttcttccgc cacgacgagg aacaggaagt catggtcctc
      541 gggagtgcga aggagcggca caagccgatc gagggtgagg ccaccgtcac cttccgtgac
      601 cagcgcgatg tggggcccgg tggcgaggcg gtcggcgagt tcatctaccg gcagacgcag
      661 ctctcgaagg atgtcttcct caaggactac aactaccgga agcccacgct ggaaatcaaa
      721 gggaccgccg acgtcatccc caagggattc ggcaccttca tggagtatgg cgatcacttc
      781 aaggatcccg aggacgggaa gcggctggcc gcgatccgcg cgcaggaaca gagctgccgt
      841 caaaaagagt ggttcggccg gggcgcgatg cgccacttcc gcgcgggctc cacgttcacc
      901 ttgcaggacc atttcgtgac gaaattcgac ggcaagtacc tgatcaccag cgtgacgcac
      961 agcgctcacc aggccggcgc ggtcggcggc gacaccggcc cgcaggggca gcgaccggga
     1021 tatctcaacc atttcgtcgc catacccgcc gacacgctgt tcatcccgcg gcggatcacg
     1081 ccccgccccc gcatctgggg atacatgaag gcgcagatcg atgccggtgg atcaggtgac
     1141 tatgccgaga tcgacgatct cgggcgatac aaggtgaaac tgccgttcga cctgtcggac
     1201 gcgaaagaag ggaaggcctc gcatttcatc cgcatggcgc agccctattc gggaggcggc
     1261 atggggatgc atttccccct gcacaagggc accgaggtgc tcctgatcca ccttgatggc
     1321 gaccctgacc gcccgatcat cgtgggaagc gtgccgaatc cggacacgat gagcccggtg
     1381 acgggagcaa accagacgca gtgcgtcatc cataccggcg gcggcaacaa gatcgtcatc
     1441 gaggataccg atggcggaca gcgcattgcg ttctcctcgc cccactccga gacgttcttc
     1501 tccctgggcg cggcgcccga gtaggccatg ggcggcggac cgggcggagc cggcatctcg
     1561 gcgggagatc ctcacggcgc ggcgggcgac gggcgcggaa gcgcgaccac cgacaagaac
     1621 ggccaggttt ccaagaccgc gggggaccat cgcgaggatt cccacaacca ggtcagccag
     1681 acacagggcg acagctggat cgaggtgaag gggaataaga atgagttcaa gatgggcgcg
     1741 tttctcgagc tcatcggcgg catgaagcac accatggtgg cgctcttcaa gaccggtctc
     1801 gacttctcct acaaccgcac gaacgacatc ggcccctaca ccaccgaaat gttcgcggcc
     1861 aagtacgaac gcctcggttc gaagaacgaa aaggtgatgg ggaagaagac cgagacctgg
     1921 accggataca agtacgagaa gaccaccggc ccctgggtgg aggccaacgc caagaaacgg
     1981 caggaggcgg aagccaagaa gaaggccatc aacgaggccc ttcaccagcg ggccggcgcg
     2041 atgctcgagg agaaggccac gaagtgcaag caacgggcgc aggtcgtcaa ggagtccgtg
     2101 gcgatgctcg accgtcgagt cagcaaggag atgcggctca cctgcgaaaa ggcgcgggag
     2161 caggttaaca agcacgaacg ggagatctcg aagatagaga tggtcaccga caagttcgag
     2221 cagaaattga aggcgatgga cgcgtcgatc ggctccttga tgtccatcga cgccaagaag
     2281 atcacgcaca aggccggcaa atacgacgcg aaggccagcg ttctcaagtt cacgagcgac
     2341 ctgatcaagc tcggcgaatg agccgacgcg aagagggcgg caaaccatga ccgacggacc
     2401 ggcgccctcc gcgggcgaac ccacccacaa ggataccgac cccgttcccc ctgtctcgtc
     2461 cggggcgttc tggaagacga tcaaggacaa gtacgatcgg cacgagaacc atcacggggt
     2521 cttcgattcg cactcgtgga agctgatcgt cggcctgtcc gtgtcggccg tgctgtccaa
     2581 ggcgcacaag atcacgctcg gctgggagca caagtcggtc aagggcgggt cggtcaccgt
     2641 cgtgatgccg tacgacaaca agcttaatgt cggcgccgtc tgcaactaca tcatcatgga
     2701 caagagtgaa accacgctcg gcaacaagtc ggagtggatc ctcggcttca agttcgccaa
     2761 ggtgaacggc aagaaacaga agttcgaggg cgccaacaag ctcaaggtgt ccgagatcga
     2821 gcaggccaag aagacggtgg acctgcagct tcacgtcatg gcgctggaga agctgatggc
     2881 cgccgaactg gaacgcgata tcaagaatct cgaggaagag cacagcaagg ccgaggagat
     2941 catgaagaag gccgaggcca cctacacgca gatggaagtc cagatgaagg agtggacgtt
     3001 caagtccaac tacctgaagt cggacattcc gaaggcgcag tgggaatgcg actcggccga
     3061 ggccaagtat tccgcaacct ggaagagcct gtccgatgcg gctcacgaga tcacggcgga
     3121 cggcggggtg gacatggttg ccggggccga ggcgaaattc atcggcaaga gcctgatcca
     3181 gctcggcgcc tcactgacga acgccggctg atccggaacc ctgccggagc gaatcgatga
     3241 ccgtcactca agatcaggtc gacgaggcca aggccgcttc cgaggcggcc cgcaaggagt
     3301 ccgaggccgc ccacgcggcc gccgaaaagg cgaagacgga tgccgcgaag gcccgcaccg
     3361 acgccaccaa tgcccgcacc gagtccaatc ggctcaaggg ggaggtgggt ccgctcgaga
     3421 cgaaggccgg ccagctggaa ggcgaggccg cggcctcgaa gaccgcctgc gacaacgcga
     3481 aggccgccgc caccaaggcc gagtcggacg ccacggccga ggaagggcgg gcggcccgcg
     3541 tccaggccct gaatccgggc gagtgcaacg ccgacacggt ggcggccgac gggaaggcga
     3601 aggccgcccg cgacagggcc accgcgctgc gcgccgacgc cggcacgaag tgcgccaagg
     3661 ccgccgagga tgcgaagaaa gccgaagcgg cgcgcgccga ggcgacggcc gcccggacga
     3721 aggccgacgg cgccagcgac aaggccgatg atctcgagga tgccgccgac gatgccgagg
     3781 ccgcgatccc cggtgcggcc gccacggcaa tctcgatggc caccgcggcc gcggtggcca
     3841 agtccaagct cgaatccctg gaaaaggagc tcgccgccca ggccgcgaac cccggcacgg
     3901 tgggcccccg cgcgcccggg ctgaacgacg tcgcgcccat tgtcggagcc ccgccaccca
     3961 cgatcaacaa ccccggcatc cacaacgaca ccaagaagaa cgccaccatc ggggccaaga
     4021 tgggaatctt cctggtcggt ggcgacagtt acgaaaaggt cgagggaaag ctgatcgaga
     4081 tcatcgacgg gagcaagatc accgtggtcc tcggggggga tttctcgctg gtgctcgggg
     4141 ccaagctcga ggaaattgtc ggcctcgaat cgaagctcgt gctgggtgtc gatcgccggc
     4201 tcgtggtggg atttcgctgg gaacggaata ttggcatcaa ggccgagacg gtcgtcggcg
     4261 acgccaccga ggtgacgctg gcccgccacg accagttcat ccccacgctc acgaccggtg
     4321 tgaacccctc cacgcgcaag gaaaccaccc cggagatgga gaccaaggcc gccaaggtca
     4381 tggaggagta caaggcgcgc ctggtcgacc tgcgcaagaa ggtcaaggac gactttgacg
     4441 aggcgcagcg gcactaccgc gaagtctcct cgcacgtcga caagatggcg gaggaagtga
     4501 agcggctcga ggaggtgatc aaaaacctcg acctcaaggt gaaagacatg aagacgcagg
     4561 ccaagaagta cgacgagaag gcggccacgt tcatcaagat catcgtcagc ggcaccgcca
     4621 agctgaaacc gaaacgcttc aaggggaacg ccgagcagaa gttcaagatg gtggccagcg
     4681 ccatcgccaa gctccagggc gaagtgaaat ggaactgagg ctcgtatgcc gaccctgatc
     4741 aacgactcgg ggcttcgctt cggtgtcttc gtgggacgcg tgccgccggc cgcgctcacg
     4801 gccacactgg tcgtgaaggg agccttcgcg ctccgcccga acggacctgc cgaggtcatc
     4861 ccggaggagg aacagcctca cctggatggc gacaaggcct gggacgacga tcccgcccgc
     4921 ggactgcgct cggccagcga tttcgcaccg ttcaagccgg gcaccgacct cctcctcgcg
     4981 ggacatgcgc acgcgccggg ggggcgcccc gttcccttca cgcaggtgtc gttcggcgtg
     5041 gccaacttcc ggaagccggc ggtcgtgatc ggcgaccggc gcctcaagtc ggcgatggga
     5101 ggctcgagcg cgggtgagcc agagcccttt acctccatgc cgctcgattg gtcccgcacg
     5161 tggggcggat cggacgtgcc ggagaatccg gtggggcggg ggcgacacga gctcacccga
     5221 cccgacggca gtaaggtgcg actggcggca aacatcgagt cggtggacca agccacgtcg
     5281 gccagcgggg ttttcggcag gaggatgggc ttcgggccga tcgatccgaa ctgggccgag
     5341 cggatgtcgc gcgtcgggac gtacgacggg aagtggctgg ctgagcgctg gccctggtac
     5401 cccgacgact tcgactggcg cttcttcagc gcagcgccgc ccgatcagta tctccaccag
     5461 atttacctga agggggacga ggcgctcgaa ttcgtccacc tccaccccga gcacgcggtc
     5521 ctcgcctcgc ggctgccggg cctgtacgcg cgctgtttca tccgggtgcg gaagggcgtc
     5581 gatctcgaat tccgcgaggt gccgatgcgg atcgacacgc tgtttgccga cctcgatgcc
     5641 tcgcaggtca tcctcacctg gcggggcgtg acaccggccg ccacgctgaa actcaaggag
     5701 ttcgaggact acttcgtgat cctggagccc ctggccgcgc cgtcgggccg ttcgctcgac
     5761 gagtaccggg agcggcacga gcggcgcctg cgcgagatag cggccgagtt cgaggtggag
     5821 ccggtcatcc tcgagccgat cgtcatgccg accttgaccc cggccgcgac ggcctgggcc
     5881 gagcggctcg aaaaggcggc caagaaactc cgcgacgcgg cccaggtgga ggcccaggcg
     5941 ccgcccccgg ccacgggtcc cctggggacg ccgatggcgc cgctcaagat gccgccgccg
     6001 ccgaaagtga acagcatcgc cgatgccacc gcgctggcgg cagccgactt tgcggcgatc
     6061 gacggccacg cgcccggctt ctcggccaag tatccaccgc ccgacttttc cgaattcgcg
     6121 gccgacacgg ccctgttcgc cgccttcaat ggcctgcccg acatggaaat ggacgacgcg
     6181 gaagacgatc cctggacccg caagtcggtg atcgagcacc tggccaccgg cgggcgcttc
     6241 gacggcgagg acctgagcga gctcgatctc tcgggcctcg acctgtcggg ggccggattc
     6301 cgcggtgcca ccctggaggc caccaacctg gccggcgccg gcctgatcgg cgccgacttc
     6361 cgcaaggcat ctatggccgg cgccatcctg gacggcgccc gctgtgaacg ggcggacttc
     6421 ggcggtgcgg acatggccga ggtttcgggt gccgcggccg tcttcgccgg gatccagggt
     6481 gaggcatccg actggacgga agcccgattg gcc
//
LOCUS       DASVOD010000157         9617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_949502, whole genome shotgun sequence.
ACCESSION   DASVOD010000157 DASVOD010000000
VERSION     DASVOD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949502"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..2367
                     /locus_tag="VF720_03190"
     CDS             130..2367
                     /locus_tag="VF720_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7878384.1"
                     /translation="MRRWARWLHLGWPAGQVEKLPEVGPDGATSIPGVRIAGDLAGIP
                     LLKFALDSGVRAVRAIAVELGPCDRAANGSSRRDPAEGPVDLVILGAGVAGMAAAVEA
                     AKLGLRYQVIESAAPLSTLVNFPKGKPIYTYPADVTPAGELQVTATVKEALVDELEAQ
                     VARAGITTVIGRAERISRQPNDQLLVHLETGESCRARHVLVAIGRSGNHRRLMVPGED
                     RENVYNRLHDPADFAGRRVVVVGGGDSALEAAVALAEAGAAVTLSYRGETFARPKPEN
                     VAHCDARRTDADDPAPGRLRTAMGTAVIAIRVGSVDLKPVKGGAAQTIPNDDVFVLIG
                     REAPLGFLRRSKLPIAGDMTPASWTALGLFVLFCAWLYNWKSGGAMSNLWYDKGWFPT
                     NLESLTAAWSVPPTSLLGITVKSASGPSFWYTLAYSLLVTIFGVKRIRRRKTPYVTAQ
                     TLTLMAIQVVPLFLLPEIILPWLNYHALLPAGIADALFPVVNYGHGREFWRAYGLILA
                     WPLMIYNIFTDQPLAAWLILGFVQTFVLIPAMVWFWGKGAYCGWICSCGALAETLGDT
                     HRHRMPHGPGWNRLNFLGQFLLAVAFVMLGIRIAGWIWPHSWAALQFHHLKDGWKWIV
                     DVSLAGILGYGLYFWYSGRTWCRFACPLAALMHWYARLGRFRILADKKKCISCNVCTS
                     VCHQGIDIMNFANKGLPMADPQCVRCSACVQMCPTGVLSFGRVDGRGNVAAVDRLAAS
                     RARES"
     gene            2453..2887
                     /locus_tag="VF720_03195"
     CDS             2453..2887
                     /locus_tag="VF720_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEX7878385.1"
                     /translation="MGYDETLVAPMREELAQLGFKETKTAEEVQREVETRKGLTMVVV
                     NSVCGCAAGKARPGIALAMQSEVKPDNFITVFAGNDVEATAKARGYFTGYLPSSPSIG
                     LLRDGKLVFMMERHDIEGNDALTIANRLSAAFREHCGTGAGA"
     gene            complement(3009..3914)
                     /locus_tag="VF720_03200"
     CDS             complement(3009..3914)
                     /locus_tag="VF720_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4394 domain-containing protein"
                     /protein_id="HEX7878386.1"
                     /translation="MNPFVTTRRSTPGRLAPALAPLAIAAFLAVAPPATRAAEEIFAV
                     TTSNTLVNFNSSSPWFFSSRPITGMMSGENALGIDFRPALPLGRLYVLGSTGRLYRVD
                     DPFSGVAALVTTTPFALSGSSVGFDFNPTVDRIRVITDADQNIRLNPDTGNLAATDPP
                     LAYAAADANFGANPSATGGGYTNSVPAAASTLLYDIDSSLDILVTQNPANNGTLNTVG
                     SLGLDANGVNGFDISGYTGIAYAALNTGGPQSNLYTVNLATGLASDLGLVGCQEPLRG
                     LAVYRELPTPVESSTWSRIKSLTRD"
     gene            complement(4063..4626)
                     /locus_tag="VF720_03205"
     CDS             complement(4063..4626)
                     /locus_tag="VF720_03205"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX7878387.1"
                     /translation="MPRITRVYTRSGDDGTTGLGTGGRVPKDHLRIEAYGTVDELNSV
                     IGLALTADLDPWLVEVLGHIQNDLFHLGSDLCIPEADKAAMPVPTVEPRHVDELERWM
                     DTMQEELTPLENFILPGGAVGAAHLHVARTVCRRAERLAVHLSRTEPIGPLVVRYLNR
                     LSDALFVMARFENLRKGRPDISWNSRA"
     gene            4774..5085
                     /locus_tag="VF720_03210"
     CDS             4774..5085
                     /locus_tag="VF720_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX7878388.1"
                     /translation="MAYVVTGNCQDCRFTDCVEVCPVNCFYGDDKMLYINPNECIDCD
                     ACVPACPVEAIYSMDDVPGDQKAWIAANAEKTEAGNLPNITQKVDPLPTAEAKKNSLG
                     L"
     gene            complement(5089..6915)
                     /locus_tag="VF720_03215"
     CDS             complement(5089..6915)
                     /locus_tag="VF720_03215"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7878389.1"
                     /translation="MFTRWVRNWTLGALLVSATLAANTVSAALPTNFADQSVVTGLDL
                     PSGFTFLPDGRMIVIEQKTFRVRLVLAGGSTADTTLTITDVNGSGNERGLLGVAIDPQ
                     WPTRPYVYFYFTQTPTIKCHIVRYTASGDLSNGASSNLTLASRYSIITDIPDAASNHN
                     GGTLRFGIDGKLYASLGEDADACGAQNNTLLKGNILRLNIDALPAGAGGPPAKSLITP
                     PDNPFVGSANANTKLVFAFGLRNPFRFQVDSVTGRLYVSDVGQNVYEELDECVGGENF
                     GWPNFEGPAFFSNSCLATSPVGPIAWYDRSAFASASIICGGRYRRVVGGAFTFPAEYE
                     GDVFFSEYYQGFVRRLNESAGTWSPEAAPGQPDATNWATAITNVSDYARGPDGALYYC
                     KQFTAGIRRIVYTGLQPAGIAQGITATAVSSSRIDIDWNAPLNGGAVTSYDVECRIPP
                     AAFALRANVPAGATAYADTGLVASTLYEYRVLAKNAAGSWGYSGTASDTTFSTSGVPD
                     VPRPSFLTAFPNPFRGGSGVTIRADAVNGSPLRLAIFDPAGRRVRDLSAEAAATDLLG
                     GPVTVSWDGRNDDGRPARPGVYFVRFEGGGRTATHRVVLSGP"
     gene            7055..8083
                     /locus_tag="VF720_03220"
     CDS             7055..8083
                     /locus_tag="VF720_03220"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX7878390.1"
                     /translation="MTRPDIPRLTANEFHAMGPYRWLADALDRQRQGLESAVRRLESP
                     GHDDEAIHDLRVSTRRILGLLAAASDYVSGKSVRRLERRLKRTRRTLGPARDAEVGAG
                     LLKDLRDSLPSGARGAAGIMAAELLARRATLENDGGDRLRSLKLRKMTTGLTRIVRDL
                     EDRDWPVGDPPLTLSMFRARALATLRERWDDLRGLPVEPIERHDAEAQHGFRIAGKKL
                     RYTLELFAPLFPKPIERRLALLKLVQDQLGHLHDLAALSSAARHLSDRAREGGLPEEA
                     LDLAELAHRLDDERHRGLDGFRSRYEALVHPGFLPPEAPPAAAPDSPPTDASTELRVI
                     DGGREESA"
     gene            8083..8937
                     /gene="thiD"
                     /locus_tag="VF720_03225"
     CDS             8083..8937
                     /gene="thiD"
                     /locus_tag="VF720_03225"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX7878391.1"
                     /translation="MVIHLGLKPIKRPVSLSIAGSDSGGGAGIQADLITFARLGSVGT
                     TVITCVTAQNLDGVAAIHPVPPADIAAQIDAIFLGFPVGGVKTGMLYSRAIIDTVGEA
                     LEKNVITRLVVDPVMVATSGARLLNEDAIAGYAERIFPWAYVMTPNLDEAEVLLGRSI
                     RDASDMPDAARDLGERHKCAVFLKGGHLEGNQLVDVLWEKETGAVHRWTGTRIIGVGT
                     HGTGCMLSAALTARLSFGDPLVEACLRAREYVEHVLLNCTEISDGRRIPGLSVTESGP
                     IRPRPAGR"
     gene            8981..>9617
                     /locus_tag="VF720_03230"
     CDS             8981..>9617
                     /locus_tag="VF720_03230"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HEX7878392.1"
                     /translation="MIRIGHVPVDPPFVLAPMAGITDKYFRLILKRIGGVGVVTMEFI
                     SSEALTRGVERTRRMMTFAEEERPLSIQIYGSRADRMADAAQLVEDEGADIVDINMGC
                     PANKVLKGCAGCSLMGDLDNAREIIRTVRRRVTIPLTVKFRLGLRETALNFIELGKIC
                     EGEGVNAVALHGRTAKQMFSGRSDWSRVAELKQALSIPVMGNGDVNTPADAM"
BASE COUNT         1566 a         3197 c         3299 g         1555 t
ORIGIN      
        1 gcgctggacg ctccactccc gggtggtccg cccggccggc agctccacgg ggcgcgtccg
       61 gaatgccggg ggcgcggcgg ccgtaacccg gcgctgacta tccggcgata acgcgctgag
      121 tccccggtga tgagacgctg ggcccgctgg cttcacctcg gctggcccgc cggccaggtc
      181 gaaaagcttc ccgaagtggg gcccgacggc gccacctcca ttcccggcgt gcgcatcgcc
      241 ggcgacctgg cgggcatccc actcctcaag tttgcgctcg acagtggcgt ccgggcggtt
      301 cgcgccatcg ccgtcgagct gggcccatgc gatcgcgccg ctaacgggtc atcgcggcgg
      361 gatcccgccg agggccccgt cgatctcgtc atcctcggcg ccggcgtggc cggcatggcc
      421 gccgccgtcg aggctgcaaa actcggcctc cgttaccagg tgatcgagtc cgccgcgccg
      481 ctcagcaccc tggtcaattt cccgaagggg aaaccgatct acacctatcc ggccgacgtg
      541 acgccggcgg gcgagctcca ggtcacggcc accgtcaagg aagcgctggt cgacgagctc
      601 gaagcgcagg tcgcccgggc cggcatcacc acggtgatcg gtcgggcgga gcggatctcg
      661 cgccagccca acgaccaact cctggttcat ctcgaaacgg gggagtcgtg ccgggcccgt
      721 cacgtcctcg tggccatcgg gcggagcggc aaccaccgcc ggttgatggt gccgggcgag
      781 gatcgcgaga acgtctacaa ccgcctccac gatccggccg atttcgccgg acgacgcgtc
      841 gtggtggtgg gcggcggcga ctcggcgctc gaggcggccg tggcgctggc cgaagccggc
      901 gctgccgtga cgctctcgta ccgtggggaa acgttcgccc gacccaagcc ggaaaacgtg
      961 gcccattgcg acgcgcgtcg caccgatgcc gatgaccccg ccccggggcg gctccgtacc
     1021 gccatgggca ccgccgtgat cgccatccgg gttgggtcgg tcgatctcaa gccggtcaaa
     1081 ggtggagcgg cgcagaccat ccccaacgac gacgtcttcg tcctcatcgg tcgcgaggca
     1141 cccctcggct tcctccgccg ctcgaagctg ccgatcgccg gcgacatgac gcccgcgagc
     1201 tggacggcgc tcggcctctt cgtccttttc tgcgcctggc tctacaactg gaagtcgggc
     1261 ggcgcgatgt ccaacctctg gtacgacaag gggtggtttc cgaccaacct cgagtcgttg
     1321 acggcggcgt ggagcgtgcc gccgacgagc ctcctcggca tcacggtgaa atcggcgtcg
     1381 gggccgtcgt tctggtacac gctggcctat tcgctcctcg tcaccatctt cggagtgaag
     1441 cgcatccgtc gaaggaagac gccttacgta accgcgcaga cgctgacgct gatggccatc
     1501 caggtcgtgc cgcttttcct cctcccggaa atcatcctgc catggctgaa ctaccacgcc
     1561 ctgctgccgg ccgggatcgc agacgccctc tttcctgtgg tgaactacgg gcacggccgc
     1621 gaattctggc gcgcctacgg cctcatcctg gcgtggcccc tcatgatcta caacattttc
     1681 accgaccagc cgcttgccgc ctggctgatc ctcggcttcg tgcagacctt cgtcctgatc
     1741 ccggccatgg tctggttctg gggaaagggg gcctactgcg gctggatctg ttcgtgcggc
     1801 gcgctcgccg aaacgctggg cgacacgcat cgtcacagga tgccgcacgg ccccggatgg
     1861 aaccgactca acttccttgg ccagttcctg ctggccgtgg cgttcgtcat gctcggcatc
     1921 cggatcgccg gatggatctg gccgcattcg tgggccgccc tccagtttca tcatttgaaa
     1981 gatggctgga agtggatcgt cgatgtctct ctggccggca tcctcggcta cggtctttat
     2041 ttctggtact caggacgtac ctggtgccgc ttcgcgtgcc ctctggccgc gctcatgcac
     2101 tggtacgccc ggctcggccg cttccgcatc ctggccgaca agaagaagtg catctcctgc
     2161 aatgtgtgca cctcggtctg ccaccagggg atcgacatca tgaacttcgc caacaagggg
     2221 ttgccgatgg ccgaccccca gtgcgtccgc tgctcggcct gcgtacagat gtgcccgacg
     2281 ggtgtcctgt cgttcggccg ggtggatgga cgggggaacg tggcggcggt cgatcggctg
     2341 gccgccagcc gggcccggga atcgtgatag actgaacctc ttctaaagga ttgcaaagga
     2401 tgggccgaaa gcgacaaccg gcctccgatt cccgccaaaa ggagattttg caatgggtta
     2461 cgatgagaca ctggtcgcgc cgatgcgcga ggaactggcc cagctcggtt tcaaggaaac
     2521 gaagacggcc gaagaggtgc agcgcgaggt ggagaccagg aagggactca cgatggtggt
     2581 cgtcaattcg gtctgcggtt gcgccgccgg caaggcccgc cccggcatcg cacttgccat
     2641 gcagtccgag gtgaagcccg acaacttcat cacggttttc gccggcaacg acgtcgaggc
     2701 cactgccaag gcgcgcggct acttcaccgg ctaccttccc tcgtccccgt cgatcggcct
     2761 cctccgcgac ggcaagctcg tcttcatgat ggagcggcat gacatcgagg ggaatgacgc
     2821 gttgaccatc gcgaaccggc tttccgccgc gttccgtgag cactgcggga ccggcgccgg
     2881 cgcctgatcg cacgacccgc ggctcgccgc gcccgcatca cgaaaatcga aggcccacct
     2941 ggtcgaaacc gggcgggcct tctttttgtc cccggatgga tcaatagccg ccgggctccc
     3001 tccgaagact aatcgcgggt caggctcttg atccggctcc aggtggacga ctccaccgga
     3061 gtcgggagct cgcgatagac ggcgaggcca cgcaggggct cctggcagcc gaccaggccc
     3121 aggtcggagg cgagaccggt ggccaggttc acggtgtaaa ggttcgactg ggggccaccg
     3181 gtgttgagcg ccgcgtaggc gatgccggtg taacccgaga tgtcgaagcc gttcacgccg
     3241 ttcgcgtcga ggccgagcga gccgaccgtg ttgagcgtgc cgttgttggc cgggttctgc
     3301 gtcaccagga tgtcgaggct ggagtcgata tcgtacagga gcgtcgaggc ggccgccggt
     3361 acgctgttgg tgtagccgcc gcccgtcgcc gacgggttgg ccccgaagtt ggcatccgcc
     3421 gccgcatacg ccaggggcgg atcggtggcc gccaggttcc ccgtgtcggg attgagacgg
     3481 atgttctggt ccgcgtcggt gatgacccgg atgcggtcga cggtggggtt gaaatcgaag
     3541 ccgacgctgc ttccgctcaa cgcgaacggc gtggtggtga cgagcgcggc cacgcccgag
     3601 aacggatcgt cgacgcgata gagccgaccg gtgctgccga gcacgtagag ccgtccaagg
     3661 ggcaatgccg ggcggaagtc gatgccgagc gcgttctcgc cggacatcat cccggtgatc
     3721 gggcggctcg agaagaacca gggcgaggag ctgttgaagt tcaccagcgt gttgctcgtg
     3781 gtcaccgcga agatctcctc ggccgcgcgc gtggcgggtg gcgcaacggc caggaaggcg
     3841 gcaatcgcga gcggggccag cgccggcgcg agacggccgg gcgtggatcg gcgagtggtg
     3901 acaaacggat tcatggcgtt ccccttctgt ggatggcgca tatcgaagtg gctggaacgc
     3961 cgaaggacgg gcaggcccgg tcgtctgcac cagctccccg gctacgcgtc ctctacggac
     4021 gcacgattcg atcggatttg ccggttgagc ggattttttt cgtcaggccc gactgttcca
     4081 ggaaatgtcg ggccgccctt tccgcaggtt ctcgaagcgg gccatgacga agagcgcatc
     4141 ggagagccgg ttgagatagc gcacgacgag aggaccgatc ggttcggtgc gcgaaaggtg
     4201 cacggccagt cgctcggccc gccggcagac ggtgcgggcc acgtgcaggt gggcggcgcc
     4261 caccgcaccg ccgggaagaa tgaaattctc gagcggcgtc agctcttcct gcatcgtgtc
     4321 catccacctt tcgagttcgt ccacgtggcg cggttcgacg gtggggacgg gcatggcggc
     4381 cttgtcggcc tccgggatgc agagatcgct gccgaggtgg aagaggtcgt tctggatgtg
     4441 cccgaggact tcgacgagcc aggggtcgag atcggccgtc agtgccaggc cgatcaccga
     4501 gttgagttcg tcgaccgtgc cgtaggcctc gatgcgcagg tgatccttcg gcacccgccc
     4561 gccggtgccg aggccggtcg taccgtcgtc gccggagcgc gtgtacacac gggtgatgcg
     4621 gggcattggg cacctcggga gggcgggtcc ggcacttgtg gagcggaaac gggttgccgt
     4681 atcttaaccg atcttgtcgg cccggcaacc gtatccgtcc gtcacaaacg gctccgccgg
     4741 gacggccggt cgaacctatt ccaggaggaa tcgatggcgt acgtcgtcac cggcaattgc
     4801 caggattgcc gctttaccga ctgcgtggaa gtctgtcccg tcaactgctt ctacggcgac
     4861 gacaagatgc tgtacatcaa tccgaacgag tgcatcgact gcgatgcctg cgtgccggcc
     4921 tgccccgtcg aagccatcta ttcgatggac gacgtgccgg gcgaccagaa ggcctggatc
     4981 gccgccaacg ccgaaaagac ggaagcgggc aacctcccga acatcaccca gaaggtcgac
     5041 ccgcttccta ccgccgaggc caagaagaac tcgctcggcc tctagtcatc agggcccgga
     5101 cagcaccacc cggtgcgtcg ccgtacggcc gcccccttcg aaccggacga agtacacgcc
     5161 cggtcgggcc gggcggccgt cgtcgttccg gccatcccac gacacggtga ccggcccccc
     5221 caggagatcc gtggcggcgg cctccgcgct gagatcacgc acgcggcgtc ccgccggatc
     5281 gaagatggcc agccggagcg gtgatccgtt cacggcatcc gcccggatcg ttacgccgga
     5341 gccgccacgg aacgggttgg ggaacgcagt gaggaacgac gggcgcggta cgtcgggcac
     5401 gccgctcgtg gagaacgtgg tgtccgaggc cgtgccgctg tagccccatg agccggcggc
     5461 attcttcgcc agaacgcggt actcgtacag ggttgaggcc accagcccgg tgtcggcata
     5521 cgcggtcgcg cccgcgggaa cgttggcccg gagcgcgaag gccgccggcg gaatccggca
     5581 ctcgacgtcg taggaagtca ccgcgccgcc gttcagcggg gcgttccagt cgatgtcgat
     5641 ccgcgatgac gaaacggccg tggccgtgat cccctgcgcg atgcccgccg gctgcagccc
     5701 ggtatagacg atccgccgga tgccggcggt gaactgcttg cagtagtaga gcgccccgtc
     5761 ggggccgcgt gcgtagtccg acacgttggt gatggcggtg gcccaattcg tggcatccgg
     5821 ctggcccggc gccgcctccg gcgaccaggt gccggccgac tcgttcaggc gccggacgaa
     5881 cccctggtag tactcgctga agaagacgtc tccctcgtat tccgccggaa acgtgaaggc
     5941 gccgccaacc acgcggcgat agcgcccgcc gcagatgatc gacgcgctgg cgaaggcgct
     6001 ccggtcatac caggcgatcg ggccgaccgg cgaggtggca aggcacgagt tcgagaagaa
     6061 cgccggcccc tcgaagttgg gccagccgaa gttctcgccc cccacacact cgtcgagctc
     6121 ctcgtagaca ttctgtccca cgtccgagac atagagtctg ccggtcaccg aatccacctg
     6181 gaatcggaac ggattgcgca ggccaaaggc gaaaacgagc ttggtgttgg cgttggcgga
     6241 gccgacgaac gggttgtccg ggggggtgat gaggctcttg gccggcgggc cgcccgcgcc
     6301 ggcggggagc gcgtcgatgt tgaggcgcag gatgttcccc ttgaggagcg tgttgttctg
     6361 ggcgccgcag gcgtcggcat cttcgccgag gctggcgtag agcttgccgt cgatgccgaa
     6421 ccggagcgtg ccgccgttgt ggttgctggc cgcgtcgggg atgtcggtga tgatgctgta
     6481 gcggctcgcc agcgtgaggt tgctggaggc cccgttcgac aggtcgccgc tggccgtgta
     6541 gcgcacgatg tgacacttga tggtcggcgt ctgcgtgaaa tagaagtaga cgtacgggcg
     6601 cgtgggccac tgcgggtcga tggccacgcc cagcaggccc cgctcgttgc ccgagccgtt
     6661 cacgtcggtg atggtgagcg tcgtgtccgc ggtggagccg ccggccagga caagccgcac
     6721 gcggaaggtt ttctgttcga tgacgatcat ccggccgtcg ggaaggaacg tgaagccgga
     6781 ggggaggtcg aggccggtga cgaccgactg gtcggcgaag ttggtgggaa gggcggcgga
     6841 aaccgtgttg gcggcaagcg tggccgacac gaggagagcg cctagggtcc agttacgaac
     6901 ccatcgagtg aacatgggat gacctcctca agcggccatg cgtcgaggtg cgaagaacgg
     6961 gccgaaccat tctaggcacc cttccaatgc cggagcaacc ggcgatgggg aggcccccga
     7021 tcgcgctatg atggtggaac cactcgaggg gaaaatgacc cgcccggaca tcccgcgact
     7081 caccgcaaat gaattccatg ccatgggccc gtaccgctgg ctggcggatg cgctggatcg
     7141 ccagcgacag gggctcgaat ccgccgtgcg gcggctcgaa tcgcccggcc acgacgacga
     7201 ggccatccac gatctacggg tgagcacacg ccgcatcctg gggctcctgg ccgccgcctc
     7261 cgattacgtt tccggcaagt cggtgcgacg cctcgagcgg cgactgaagc gcacgcggcg
     7321 cacgctcggc ccggccaggg atgccgaggt gggggccggc ctcctcaagg acctgcgcga
     7381 ttcgttgccg tccggggcaa gaggcgcggc cggcatcatg gctgccgagc tcctggctcg
     7441 ccgggccacc ctcgagaacg acggaggtga tcgcctccgg tcactcaagc tcaggaagat
     7501 gacaaccggg ctcacccgga tcgtgcgcga tctcgaggat cgcgactggc cggtcggcga
     7561 tccacccctc accctttcca tgttccgcgc gcgggcgctc gccacactcc gcgagcggtg
     7621 ggatgacctc cgcgggctgc cggtcgagcc gatcgaacgg cacgatgcgg aagcgcagca
     7681 tggctttcgc atcgccggaa agaagctccg ctacacgctc gaactcttcg cgccactctt
     7741 tccgaagccg atcgagcggc gcctcgcgct cctcaagctg gtgcaggacc agttgggcca
     7801 cctgcacgac ctggcggccc tctcgagcgc ggcgcgccat ctttccgacc gggcgcggga
     7861 gggcggtctc cccgaggagg cgctcgacct cgccgaactg gcccaccgcc tggatgacga
     7921 gcgccatcgc ggcctcgacg ggttccgctc gcggtatgag gcgctggtcc atcccggctt
     7981 cctgccgccc gaagcgcccc cggcggctgc gccggacagc ccgcccaccg acgcgtccac
     8041 cgagctgcgg gtcatcgacg gcggccgcga ggaatcggcc tgatggtgat tcacctgggc
     8101 ctcaagccga tcaagcgacc ggtgtcgctc tccatcgccg ggtcggactc gggaggcggt
     8161 gccggcatcc aggccgatct catcaccttt gcgcggctcg gatcggtcgg caccacggtg
     8221 atcacctgcg tcacggccca gaacctggac ggcgtcgccg ccatccaccc ggtgccgccc
     8281 gccgacatcg ccgcgcagat cgacgcgatc ttcctcggct tcccggtcgg tggcgtgaag
     8341 acagggatgc tctactcacg cgcgatcatc gacacggtgg gggaggcact cgagaagaac
     8401 gtcatcaccc ggctcgtcgt cgacccggtg atggtggcca ccagcggcgc gcgactcctg
     8461 aacgaggacg ccatcgccgg ctatgccgaa cgcatcttcc cgtgggccta cgtgatgacg
     8521 cccaacctcg acgaggccga ggtgctcctc ggccgctcga tccgcgacgc gtccgacatg
     8581 cccgacgcgg cccgggacct gggagagcga cacaagtgcg cggtgttcct caagggtggg
     8641 cacctcgagg ggaaccagct ggtggacgtc ctctgggaaa aggaaaccgg agccgtgcat
     8701 cgctggaccg gcacacggat catcggtgtg ggcacgcacg gcaccgggtg catgctttcg
     8761 gccgccctga cggcccgact gtcgttcggc gacccgctcg tcgaggcgtg ccttcgggcc
     8821 cgggagtacg tggagcatgt gctgctgaat tgcacggaaa tctcggacgg gcggcggatt
     8881 ccgggcctct cggtgacgga gtccgggccg atccggccgc ggccggccgg ccggtaggcc
     8941 ccccggccct cccgcgccca tccgctatac ttcccctccc atgattcgca tcggacacgt
     9001 ccccgtcgac ccgccgttcg tcctggcccc catggccggc atcacggaca aatacttccg
     9061 gctgatcctg aaaaggatcg gcggggtggg cgtggtgacc atggagttca tctcgtcgga
     9121 ggcgctcacc cgcggggtgg agcgcacgcg ccgcatgatg accttcgccg aggaagagcg
     9181 ccccctctcg atccagatct acggcagccg cgccgaccgg atggccgatg ccgcccagct
     9241 cgtggaagac gaaggggccg acatcgtcga catcaacatg gggtgcccgg ccaacaaggt
     9301 gctcaagggt tgcgcgggct gcagcctgat gggcgacctg gacaacgcca gggagatcat
     9361 ccgcacggtc cgccgccgcg tgacgattcc gctcacggtg aagttccggc tcggcctccg
     9421 ggagacggcg ctcaacttca tcgagctggg gaagatctgc gaaggggaag gcgtgaacgc
     9481 cgtggcgctc cacggacgca cggccaagca gatgttctcg gggcgttcgg actggagccg
     9541 cgtcgccgag ctcaagcagg ccctctcgat ccccgtgatg gggaacggcg acgtgaacac
     9601 ccccgccgac gcgatgg
//
LOCUS       DASVOD010000158         6579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_635977, whole genome shotgun sequence.
ACCESSION   DASVOD010000158 DASVOD010000000
VERSION     DASVOD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6579
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635977"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2528)
                     /locus_tag="VF720_03235"
     CDS             complement(<1..2528)
                     /locus_tag="VF720_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878393.1"
                     /translation="MFAARAFSLVIAAMLLVAIAAPARAQRGIEAIPTEPAPVPEVDD
                     GPSRPFPPFRSNGLPGLATTPIGSPNVRMNDPSGDGSTEVQSAPQIAAAQNRVFACWF
                     DSRGLDAPTTTPPNTLVGFGWSTNYGQTWIDGGNMPRLTSDSQVLNARLTGDGQGNFY
                     LVARVTTNLLGTNGTSIGLWKGTFTGSSFAWGTPTYLNSLAGTTEILDNPFVLVAPGS
                     PVVYVAWTRIMNGLAQGRIEIIRSDDAGLNWTAPATVAQDYSGDRRPGHARLALGPSG
                     EVYATWLAFSDFIYFQCNIDDNAQAYEPIYFSRSVDSGATWTAPVTVLNQGIMVRPIV
                     GPGETASSEGMYPSFATDNTGGPGNGVLYVTAPHSPDFSLGATSGITRPEVEANDFPA
                     QAASVLQPGDEASGTWTSTADNDYWPIDLTAGQTVFLHLEPPAFTCPSTNGMTVTLRL
                     YAADIPLANVHPDTLLGTQTRTSGAPSWLMFTCQRTGRYFIRAFTGSASALNQDYRLR
                     SRLVSFAAPTANNPARDTKDIVLCLSTDQGSTWSQPVRINTSPAGLVEGQPNVITDEK
                     GALHIFWYDRRESVLNPGLVNQAQPTDIYLYSKNPLELPQNRRISDQSGAFAGLSRST
                     TYPGNFGGLWQAGGRLYAAWADGRSRSGLGGTSVDTWVNVLDFYPPQTTVVSGPAEGD
                     TLFARSVTFVASGMDSVTAMAALTYETQMDGGGWVARGTTTSFTYNDMTPGAHTFQVR
                     ASDAAANTDATPVTVGFHVSPRITWANPAGGSWNTAANWNTNTVPGSGDEVEITLAGA
                     YTVSLGSSATVSLLMLGGAGSTPTLDIANATLTATTGTTVASGAT"
     gene            3037..4347
                     /locus_tag="VF720_03240"
     CDS             3037..4347
                     /locus_tag="VF720_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7878394.1"
                     /translation="MELRRSTPAGPARPARASAPQDRFVGRSRAIRSVLSDLSKVAGT
                     RCAVLIEGESGTGKELTAHVIHEMSAHRSGPFEALNCGAIPENLVESELFGHVAGAFT
                     GAMRQHRGAFERAAGGTVFLDEVAELPLNAQVKLLRVLQEQNFRPVGGEKLVSVQSRV
                     LAATNRDLKQEVRTGRFREDLYYRLSVFPIRLPPLRDRRDDIPLLIEEFLRDVSVELG
                     CRRPDIEPVALQRMLLYSYPGNIRELQNLIRTLIIEARDAGRIMDRHVVALFRRHQIE
                     ELHPARLVDGAGPCPGPPGMPSDAGTWVVEQLRRFDFNIALAERKLKATKYALSGSGH
                     RVACSRSGLTYYLQGEWIRALPRESWDQRAVAIRLAGNTELAGRLTIKLDHFLKRTLA
                     AMQPTRSSAQRLRALSESFRKLPGCYEPDLAHFVAQFPKSPWGP"
     gene            complement(4512..6548)
                     /locus_tag="VF720_03245"
     CDS             complement(4512..6548)
                     /locus_tag="VF720_03245"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HEX7878395.1"
                     /translation="MTPFAPRKSSRHGLLGAVGAIVCLAVLVLLQALGAFRTADMKVR
                     DARYLLRGPRPAADAIALVTIDDATIEAYGRWPLPRSQYAVLIGALEEAGARVIGFDL
                     LLFDPLDPADDSTLVILTGEFDNVVHAYVFPPRNSDLEAQRFLPEARASRFVREGIAA
                     EPLPVWQASEIALPFDDLLFTSPALGHVAVLLDRDGPVRRVPFLVGFRDRVYPALALR
                     MVGLARGDSSLPVAARTRHGVSITWQDGTRLDVPVDGEGGTTLDYAGDIEAFPHRVSM
                     LDVVQWYQAADTTRLAAAFANRIVLVGNTAVGEAAADIGTTPFSNKTPLVYVHANALD
                     ALLGGRFVHHAPPLLFYGFWALAAALLGWLVMTRPLLHAAVGVVAVTLAWQAAAYALL
                     VFRRIDMPSLLPALLPFTIYATLGTFRLFFVERHARETDRELKVARDIQQKLLPQEPP
                     AAPEFDVFGTNLPAQEVGGDYFDWLSGNGKGLLVALGDVSGKGVSASLLMSHLRASLH
                     VEARNNASPADIAREMHRSMYTAVERGRFATFFLARLARPTGELVFCNAGHNPPILVS
                     ARGVELLEATGLPLGLMDIDDYEEGSRPFDPGDVLVVFSDGVTEAPSGSELYGDDRLL
                     DLVTRLADGRRTSSEIGQAILEDVAAFTRRGNDWDDVTVLVVRRKSDADPTSSA"
BASE COUNT         1065 a         2132 c         2338 g         1044 t
ORIGIN      
        1 gtggcgccgg aggccaccgt cgtgccggtc gtggcggtga gggtggcatt cgcgatgtcc
       61 agcgtcggcg tgctccccgc gccaccgagc atgagcaggc tgaccgtcgc cgaactgccg
      121 agcgacacgg tgtaggcgcc ggccagcgtg atttccactt catcgcccga ccccggcacc
      181 gtattggtgt tccagttggc cgcggtgttc cagctcccgc ccgccggatt ggcccaggtg
      241 atgcgagggc tgacatggaa accgacggtg accggcgtcg cgtcggtgtt ggcggccgca
      301 tcggaagcgc gcacctggaa cgtgtgcgcc ccgggcgtca tgtcgttgta ggtgaacgag
      361 gtcgtcgtgc cccgtgccac ccatccaccg ccgtccatct gcgtttcgta cgtcagggcg
      421 gccatggccg tgacggagtc catccccgac gccacgaacg tgacggaacg ggcgaaaagc
      481 gtgtccccct cggccggccc gctcacgacc gtggtctgcg gcgggtagaa gtcgaggacg
      541 ttcacccagg tgtcgacgga cgtgccaccg agaccggacc ggctgcggcc atcggcccat
      601 gccgcgtaga ggcggccgcc ggcctgccag aggccgccga agttgccggg ataggtcgtt
      661 gaccgcgaca ggccggcaaa ggctcccgac tggtcggaga ttcttcggtt ctgcggcagt
      721 tcgagggggt tcttgctgta gagatagatg tccgtgggct gggcctggtt gacgagtccc
      781 gggttgagta cagattcccg ccggtcatac cagaagatgt ggagtgcccc cttttcgtcg
      841 gtgatgacat tcggctgccc ctcgaccagg ccggccggcg atgtgttgat acggaccggc
      901 tggctccagg tcgacccctg gtcggtcgaa aggcacagca cgatgtcctt ggtgtcgcgc
      961 gccgggttgt tcgccgtggg cgcggcgaag ctcaccagcc tcgagcggag ccggtagtcc
     1021 tggttgagcg cactggccga tcccgtgaag gcccggatga agtaccggcc ggttcgctgg
     1081 caggtgaaca tcagccagga gggagccccg ctcgtccgcg tctgggtgcc caggagcgta
     1141 tccgggtgga cgttggccag ggggatatcg gcggcataga ggcggagcgt gacggtcatc
     1201 ccgttggtgc tcgggcaggt gaatgccgga ggctcgaggt gcaggaagac ggtctggccg
     1261 gccgtcaggt cgatcggcca gtaatcgttg tcggcggtcg acgtccaggt gccggacgcc
     1321 tcgtcccccg gttgcagcac gctggcggcc tgggccggga agtcgttggc ctccacttcc
     1381 ggacgagtga tgcccgaagt ggcgccgagg ctgaagtcgg gtgagtgggg cgccgtcacg
     1441 taaaggacgc cgtttcccgg accgccggtg ttgtcggtgg cgaacgacgg gtacatgccc
     1501 tcggagctgg ccgtctctcc tggccccacg atcgggcgga ccatgatccc ctggttcaat
     1561 accgtgaccg gcgccgtcca ggtggcgccc gaatccacgc tccgggagaa atagatcggc
     1621 tcgtaggcct gggcattgtc atcaatgttg cattggaagt agatgaaatc cgagaaggcc
     1681 agccaggtcg cgtacacctc accggatggc ccgagcgcca gacgcgcgtg gccggggcgg
     1741 cggtcgccgg agtagtcctg ggccacggtg gccggtgcgg tccagttcag cccggcgtca
     1801 tccgagcgga tgatttcgat gcgcccctgc gccaggccgt tcatgatgcg cgtccaggcc
     1861 acgtagacca ccggtgagcc gggtgccacg agaacgaagg ggttgtcgag aatctccgta
     1921 gtgccggcaa ggctgttcag gtaggttggc gtgccccacg caaacgagga tcccgtgaac
     1981 gtccccttcc acaacccgat ggacgtgccg ttcgtcccca gcagattcgt ggtgacgcgg
     2041 gccacgagat agaagttgcc ctggccgtcg ccggtcaggc gggcgttcag cacctgggag
     2101 tcggacgtga ggcgcggcat gttgccgccg tcgatccagg tctgcccgta attggtggac
     2161 cagccgaagc cgacaagcgt gttggggggt gtggtggtcg gggcatccag tcccctactg
     2221 tcgaaccagc aggcaaacac acgattctgg gccgcggcga tctggggagc gctctggacc
     2281 tcggtcgatc cgtcaccaga agggtcgttc atccggacgt tcggacttcc gatcggcgtc
     2341 gtggccagcc cgggcagacc gttcgatcgg aacggcggga acgggcgcga tggcccgtcg
     2401 tccacctccg ggaccggggc gggttcggtc ggtatggctt cgatcccgcg ctgggcccgg
     2461 gcgggcgccg caatcgcgac gaggagcatt gcggcgatga cgagcgaaaa tgcgcgcgcc
     2521 gcaaacaacg cacggcttga acgattcgca actgcggcca taccggacat gatgcaagcc
     2581 tccccggttg ggtggatggt ttccctgtga atgcgtgaaa tgtggccgcc acgcaccagc
     2641 tggccattgt ttgtcgaaac aaccgtcgac ggatggcagg catggcgccg cgttggcgtg
     2701 acgtgacttg aatttgtcaa gcatcaagtc aggccggtgc cgtcggatcg gcggatcggg
     2761 cgtgccgggc agagcacggg gagccgtgga ggcggcggaa cgaacgaatc gggcgtgact
     2821 tgagtactta cggagtagcc ggggtctgtg gggtgcggcc cctcccggcg tgctgcgact
     2881 ctcttccgat tgtatcccct ccctttcact ttcctgaacg attgttcgcg ctattttcgt
     2941 tagtgaagaa aagagtttgc ggaattccta ttgaagtgac gcattggtag tgacaagtgg
     3001 tcactacacc cgacgaaaag gggaccaggg agagatatgg aactacgacg cagtacgccg
     3061 gccggcccgg cccgcccggc acgtgcatcc gcaccccagg accgcttcgt cggccggagc
     3121 cgcgcaatcc gctcggtcct gagcgacctc tccaaggtgg ccggcacccg ctgtgccgtc
     3181 ctcatcgagg gggaatcggg gacggggaag gagctcacgg cgcacgtcat tcacgagatg
     3241 tcggcgcacc ggtcggggcc gttcgaggcc ctgaactgcg gcgccatccc ggaaaacctg
     3301 gtcgagagcg agctgttcgg ccacgtggcc ggcgcgttca ccggggccat gcgccagcac
     3361 cgcggcgcgt tcgagcgcgc ggccggcggc acggtcttcc tcgacgaagt ggccgagctc
     3421 cccctcaacg cgcaagtcaa gctgctgcgc gtgctgcagg agcagaactt ccgccctgtc
     3481 ggtggtgaaa agctcgtcag cgtgcagtcg cgcgtgctgg cggccaccaa ccgggacctg
     3541 aaacaggaag tgcggacagg tcggttccgc gaagacctct actatcgcct gagcgtcttc
     3601 ccgatccgac tgccgccgct gcgagaccgt cgtgacgaca ttccgctcct catcgaggag
     3661 ttcctgcgcg atgtgtcggt cgagctcggc tgtcggcgtc ccgacatcga gcccgtggcg
     3721 ctgcagcgga tgctcctgta ctcctacccg gggaacatcc gcgagctgca gaacctgatc
     3781 cgaacgctga tcatcgaggc gcgcgatgcg ggtcgcatca tggaccgaca cgtggtggcg
     3841 ctcttccgcc gccaccagat cgaggagttg catcccgcgc gcctcgtgga cggtgcgggg
     3901 ccgtgccccg ggccgccggg tatgccgtcc gacgccggta cgtgggtggt cgagcagctc
     3961 cggaggttcg atttcaacat cgcactggcg gagcggaagc tgaaggccac caagtacgcc
     4021 ctttcgggaa gcggccaccg ggtggcctgt tcgcgcagcg gcctcaccta ttacctgcag
     4081 ggagagtgga tccgtgccct gccccgggaa tcgtgggacc agcgtgcggt ggcgatccgc
     4141 ctggcgggca acaccgaact cgccggccgc ctcacgatca agctcgatca ctttctcaag
     4201 cggacactgg cggccatgca accgacacgc tcttccgccc agcggctgcg cgcgctcagc
     4261 gaatcctttc gaaagctccc cggctgctac gagcccgacc tcgcccattt cgtggcgcag
     4321 ttcccgaaga gtccctgggg accctgaccg aagccagcag cttcggcgcc agggccgcca
     4381 cctgtttggg agtaagggcc gccacctgct tcggcgtcat cgccgcgacc agccttgcca
     4441 ggcgtgtcgg agcgaggtcc gccagctcgc cgggggacag ggccgcgagg tcgagatcgt
     4501 tttcccggga atcaggcgct tgatgtcggg tcggcatcac tcttcctccg gacgacgagc
     4561 accgtcacgt cgtcccaatc gttgccgcgg cgcgtgaacg ccgccacgtc ttccaggatg
     4621 gcctgcccga tctcgctgga ggtgcgccgg ccgtcggcca gccgcgtcac caggtccagg
     4681 aggcggtcgt cgccgtagag ttcgctgccg gacggcgcct ccgtgacgcc gtcggaaaaa
     4741 acgacgagca cgtcgcccgg gtcgaagggg cggctccctt cctcgtagtc gtcgatatcc
     4801 atgaggccca gcggcaggcc ggtggcctcg aggagctcca cgccccgggc cgagacgaga
     4861 atcggcgggt tgtggccggc gttgcagaag acgagctcgc cggtcggccg cgccagtcgc
     4921 gccaggaaga aggtggcgaa gcgtccccgc tccacggcgg tgtacatcga gcgatgcatc
     4981 tcccgggcaa tgtcggccgg gctcgcattg ttgcgcgcct ccacgtgcag cgaggcccgg
     5041 aggtgcgaca tgaggagcga ggccgaaacc cccttccccg atacatcgcc cagcgccacc
     5101 aggagcccct tgccgttgcc ggacagccag tcgaagtagt cgcccccgac ttcctgggcc
     5161 gggaggttgg tgccgaaaac gtcgaactcc ggcgcggccg gcggctcctg cggcaggagc
     5221 ttctgctgga tgtcgcgcgc caccttcagt tcgcggtcgg tctcccgggc gtgccgctcc
     5281 acgaagaaga gacggaaggt gccgagcgtg gcgtagatgg tgaagggcag gagcgccggc
     5341 aggagcgatg gcatgtcgat ccgccggaag acgaggaggg cataggccgc ggcctgccag
     5401 gcgagcgtga cggccacgac gcccacggcg gcgtgcagga gcgggcgcgt catcaccagc
     5461 cagccgagta gggccgctgc gagcgcccag aatccgtaga agaggagcgg cggcgcgtgg
     5521 tgcacgaacc ggccgccgag gagcgcgtcg agcgcgttgg cgtgcacgta gacgagcggc
     5581 gtcttgttgc tgaagggggt cgtgccgatg tcggcggccg cttctcccac ggcggtgttg
     5641 ccgaccagca cgatccggtt cgcgaaggcc gcggccagac gcgtggtgtc ggccgcctgg
     5701 taccactgca ccacgtcgag catcgacacc cggtggggga aagcctcgat gtcccccgcg
     5761 tagtcgagcg tcgtgccccc ttccccgtcc accggcacgt cgagccgcgt accatcctgc
     5821 caggtgatcg acacgccgtg gcgcgtgcgg gcggcaaccg gcaggctcga atcgccgcgg
     5881 gccaggccaa ccatccggag cgccagcgcc gggtagaccc ggtcgcggaa ccccaccagg
     5941 aacggcacgc gtcgtacggg gccatcgcga tcgaggagca ccgccacgtg acccagcgcg
     6001 ggagaggtga agaggaggtc gtcgaacggc agggcgatct cgctggcctg ccagaccggc
     6061 agcggttcgg ccgcgatccc ttcgcggacg aaccggctgg cccgggcctc gggaaggaag
     6121 cgctgcgcct cgagatcgga attgcgcggc gggaagacgt aggcgtggac cacgttgtcg
     6181 aactcgccgg tcaggatcac cagcgtcgag tcgtcggcgg ggtcgagggg gtcgaacagc
     6241 aggaggtcga aaccgatgac gcgggccccc gcctcttcca gcgcgccgat caacacggcg
     6301 tactggctgc gcggcagggg ccagcgcccg taggcctcga tcgtggcgtc gtcgatcgtg
     6361 acgagcgcga tggcgtcggc ggccgggcgc ggcccgcgca ggaggtagcg cgcatcgcgc
     6421 accttcatgt ctgccgtgcg gaacgccccc agcgcctgca gcaggaccag cacggccagg
     6481 cagacgatgg ccccgaccgc gccgagcaac ccgtggcgcg acgacttcct gggagcgaac
     6541 ggcgtcatcg gggacttcta ccgccgccag tacagggtg
//
LOCUS       DASVOD010000159         4055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_844927, whole genome shotgun sequence.
ACCESSION   DASVOD010000159 DASVOD010000000
VERSION     DASVOD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844927"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VF720_03250"
     CDS             complement(<1..1041)
                     /locus_tag="VF720_03250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878396.1"
                     /translation="MIPTPIAAALSPRYAIRRELGRGGMARVYLADDVRHGRQVAIKT
                     MLPEVSAAIGRDRFLREIETAARLTHPNILPLFDSGEAEGHLWYVTPFIEGESLRQRL
                     TRDGPLSVPDAVRVIRGVAAALDHAHRQGVVHRDIKPENILVADGIALVADFGIARAM
                     VADADSETSATRDVGLTGSGMTVGTPRYMAPEQFGRADVDGRADLYAMGCVLFEMLTG
                     EPPFDGTTLADLMRAHLAEAPRSIAESRPEAGGNLSEAVRRLLAKQADERPATGAAFL
                     VLLDGARLPAAMSALPNNLPRERTSFVGREKDVATAGRLLDEARLLTITGIGGCGKTR
                     LALRLARERLGAF"
     gene            1224..1784
                     /locus_tag="VF720_03255"
     CDS             1224..1784
                     /locus_tag="VF720_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878397.1"
                     /translation="MVSIVSLWLPILVSAIAVFFASFLLHMVIPFHRADYGTLPNEDR
                     AMEALRSLAIPPGDYMTPCARTPEQMKSPEFLEKFNRGPVLSMTVMKPGNMHLGSSLI
                     QWFIYSLAIGVLAAYVTSRAMGSGTDRIEVLRFAGVTGFTAYAVGLWQQSIWYKRSWA
                     TTVRYTIDGLIYALLVGGAFAWLWPR"
     gene            1950..2393
                     /locus_tag="VF720_03260"
     CDS             1950..2393
                     /locus_tag="VF720_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878398.1"
                     /translation="MSTQPATRAALALLLAGLVSCAGSTTTHRTNPEDNVRDEGSYST
                     KTTIFTDADEAALDVARGLRRDGHFEKAADAFQALHENASVDPEIREQALFDHAHLAA
                     DPLNPKKDPAMAVKLFDQFLSEFPNSKDAERAREQLKALGNRPGG"
     gene            complement(2400..3773)
                     /locus_tag="VF720_03265"
     CDS             complement(2400..3773)
                     /locus_tag="VF720_03265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7878399.1"
                     /translation="MRFTLFERLFAFLCVAVVGTATLTMDARAQGASVNRVPEAETFV
                     DLLAAGDFSSAFARFDPQMQAGLPEAQLKSIWATIQAQAGPYRNRLTARAEKAGAYDV
                     VFVPCDFEKARLDAKIVFDSEGWIAGLFFVPVPSLAGPEWQAPDYVKPDSFHEQDMTV
                     GSGEWALPGTPALPVGPGPFPALVLVHGSGPHDRDETIGPNKTFRDLAQGLASRGIAV
                     LRYEKRTKAHGKKMAGMTSLKVKEETVDDAVAAVAAVAGVTGIDPHAVFVLGHSLGGT
                     VIPRIAARTPDAAGYIVLAGATRPLEEVALEQVAYLMTLDSAGPDEMKQLESMKMELE
                     KVKALDPANADTSATFLGVPTAYWLDLRSLKPAEAAREIKQPMLILQGERDYQVTMTD
                     FRNWKEALGKRSNVTLVSYPDLNHLFVTGTGRSTPAEYLVAGHVSGTVVDRIARWVGE
                     VAGKGKE"
BASE COUNT          679 a         1439 c         1269 g          668 t
ORIGIN      
        1 gaacgcgccg agccgttccc gggccagccg cagcgcgagg cgcgtcttgc cgcacccgcc
       61 gatcccggtg atcgtgagca gccgcgcctc gtcgagaagc ctccccgccg tcgccacgtc
      121 cttttcgcgc ccgacgaacg acgtgcgctc ccgcggcaga ttgttcggca gggccgacat
      181 cgcggcggga agccttgccc cgtcgagcag caccaggaag gccgcgccgg tggccggtcg
      241 ctcgtccgcc tgcttggcca ggaggcgccg gacggcctcg gacaggttgc cccccgcttc
      301 cgggcgcgat tcggcaatcg aacggggcgc ctccgccagg tgggcgcgca tcaagtcagc
      361 cagcgtggtg ccgtcgaacg gcggctcgcc ggtcagcatc tcgaacagca cgcatcccat
      421 cgcgtacagg tcggcccgtc cgtccacgtc ggcccgcccg aactgctccg gcgccatgta
      481 gcggggcgtg cccacggtca tccccgaacc ggtcagcccc acgtcccgcg tggccgaggt
      541 ttcggaatcg gcatcggcca ccatggccct cgcgatcccg aagtcggcca ccagcgcgat
      601 gccgtcggcc accaggatgt tctcgggctt gatgtcccgg tgcaccacgc cctgccggtg
      661 cgcgtggtcg agcgccgccg ccacgccgcg tatcacgcgc acagcatccg gtaccgacaa
      721 tggcccgtca cgcgtcaacc gttgtcgcag cgattcccct tcgatgaacg gagtgacata
      781 ccagaggtgg ccttcggcct cacctgaatc gaagagcggc aggatattgg gatgcgtgag
      841 ccgcgcggcc gtctcgatct cgcggagaaa ccggtcgcgc ccgatcgcgg ccgacacttc
      901 cggcagcatc gtcttgatcg ccacctggcg accgtggcgc acgtcgtcgg ccaggtacac
      961 cctggccatg ccgccccggc cgagttcgcg ccggatggcg tagcgggggg agagggctgc
     1021 ggcgatcggg gtcgggatca tgaaatcgtg tcgctccgga cggtccaggc gcggtcgacc
     1081 ggccacgccg gcgacgtcca cgaatcgtag gggatcgctt cccgtctcgc caccatgcgc
     1141 cggctcgact agactgcccg cgccacccgc gcgaccgccc cggctccaaa caggtgtcac
     1201 gaaccccgac ccggaggaga agaatggttt cgatcgtttc cctgtggctc cccatcctcg
     1261 tttcggcaat cgccgtgttt ttcgcgagct tccttctcca catggtcatc ccgttccacc
     1321 gtgccgatta cggcacgctc ccgaacgagg acagggccat ggaagcgctg cgctccctcg
     1381 ccatcccgcc cggcgactac atgacgccgt gcgcccgcac gccggaacag atgaagagcc
     1441 cggaattcct cgagaagttc aacaggggac ccgtactgtc gatgaccgtc atgaagcccg
     1501 gaaacatgca cctggggagc agcctgatcc agtggttcat ctattcgctg gccatcgggg
     1561 tcctggccgc gtacgtcacc agccgcgcga tgggaagcgg taccgaccgg atcgaagtcc
     1621 tgcgcttcgc cggcgtcacg ggattcactg cctacgccgt cggcctctgg cagcaatcga
     1681 tctggtacaa gcggtcgtgg gccaccacgg tgcgctacac catcgacggc ctgatctacg
     1741 cgctgttggt ggggggagcg ttcgcctggc tctggccaag gtagggcggg gatcacgcaa
     1801 ccggcggtta cggcactata ataggtagca actcgtgcgc cgccgttcgt aaaaccggct
     1861 aagtcgttgg agccggccca ttcggacgat tttcaggcga ttgccggaac tcggcccgga
     1921 ccgagccggc gaaaccggag cccacggcca tgtccaccca acccgcaacc cgcgccgcgc
     1981 tcgcccttct cttggccggc ctcgtttcat gtgccggaag caccaccacg caccggacca
     2041 acccggaaga caacgtccgc gacgagggga gctactccac caagaccacg atcttcaccg
     2101 atgcggatga agccgcgctc gacgtcgcgc gcggcctgcg ccgcgacggt cacttcgaga
     2161 aggccgccga cgcgttccag gcgctccatg aaaatgcctc ggtcgatccc gagatccgcg
     2221 aacaggccct gtttgatcat gcgcacctcg ccgcggatcc gctgaacccg aagaaagacc
     2281 cggcaatggc cgtcaagctt ttcgaccagt tcctctcgga atttccgaat tcgaaggacg
     2341 ccgagcgggc ccgcgagcag ctcaaggcac tcgggaatcg gccgggggga tagggaaggt
     2401 cactctttcc ctttgccggc cacctcaccg acccacctcg cgatccggtc gacgacggtc
     2461 cccgatacgt gtcccgccac caggtattcg gccggcgtcg atcgccccgt gcccgtcacg
     2521 aagaggtgat tcaggtcggg atacgaaacg agcgtcacgt tcgatcgctt ccccagcgcc
     2581 tccttccagt tccggaagtc ggtcatcgtc acctggtagt cgcgctctcc ctggaggatc
     2641 agcatcggct gcttgatctc gcgggcggcc tcagccggct tcagggagcg gaggtcgagc
     2701 cagtaggcgg tcggcacgcc gaggaaggtg gccgaggtgt ccgcgttggc aggatcgagc
     2761 gccttcacct tttccaactc cattttcatg gattcgagct gcttcatctc gtccgggccg
     2821 gccgaatcca gcgtcatgag atacgccacc tgctccaacg ccacctcctc cagggggcgc
     2881 gtggcgccgg ccagaacaat gtagcccgcc gcgtccggcg tgcgcgcggc aatcctcggg
     2941 atgacggtgc cacccaggct gtggccgagc acgaacaccg catgcgggtc gatgccggtc
     3001 acacccgcaa ccgccgctac ggccgccacg gcgtcgtcga ccgtctcttc cttcaccttg
     3061 agggacgtca tgccggccat cttttttccg tgggccttcg ttcgcttctc gtagcgaagg
     3121 actgcaatcc cgcgcgaggc cagaccctgc gccaggtcgc gaaaggtctt gttgggcccg
     3181 atcgtctcgt cgcggtcgtg cggccccgag ccgtgcacca ggacgagggc cgggaacggc
     3241 cccgggccga ccggcaaggc gggcgtgccg ggcaacgccc actctcccga gccgacggtc
     3301 atatcctgtt cgtgaaagga atcaggtttc acgtagtccg gagcctgcca ttccggcccc
     3361 gccagggatg gcaccggcac gaaaaacagc ccggcgatcc aaccctcgga atcgaagacg
     3421 atcttggcgt cgagacgcgc cttctcgaag tcgcacggca cgaagacaac gtcgtaagcg
     3481 ccggccttct cggcccgtgc ggtcaggcgg ttccggtagg ggccggcctg ggcctggatg
     3541 gttgcccata tgctcttgag ttgcgcctcg ggaaggcccg cctgcatctg cggatcgaac
     3601 cgggcaaagg ccgaactgaa atcgccggcg gccaacagat cgacaaacgt ttcggcctcc
     3661 ggcacccggt tgaccgacgc gccctgggcg cgggcatcca tcgtcagggt ggccgtcccg
     3721 acaacagcga cacaaagaaa cgcgaacaga cgctcgaaca gggtaaatcg cactcgggtc
     3781 ctccttccgt ccgcgtatca gcgtaatccc tccacgctct cgccgtctcc cgcggcgtgt
     3841 tgggacaatc gtctcccgag tcggtagact gcgcccggaa cgcttcccag aaccgctcgt
     3901 ccccgctgca ctttccggag gtgaatccga atgaaccggg tcagcctttc actcgttgcc
     3961 gctttggtcg tgctcgggat cgagccgatt cctcatgcgg gcgccgcgtc ggattcggtg
     4021 tatgcccagc gccgcaaggc cttcgccgca aagct
//
LOCUS       DASVOD010000160         4860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_844979, whole genome shotgun sequence.
ACCESSION   DASVOD010000160 DASVOD010000000
VERSION     DASVOD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844979"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1904
                     /locus_tag="VF720_03270"
     CDS             <1..1904
                     /locus_tag="VF720_03270"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878400.1"
                     /translation="HDLIEPIAEGYRFTQGLIREVLLRELPADLRQECHSVVASYLLK
                     GYADRSDYAGPISGHLYRAGRFGEALPYLWRAATEARRLFLYDRALIYLDRALTAHTE
                     SGATDVRGTELLGARAAVLLKLGRPADARQSAQSMLTQASEGADHPDRSEAEEIVGEA
                     ALEEGDYDAAEKHLQQAFEHYQRDESATTGLPRSQRKLASLSLRRGDFDMALTRYAAA
                     RGRLEEAGDRQGVAEVRMDLGALFARRGEFEAALADLEAALVEFRALGDKHGEARTLS
                     FLASLYRRQGRLEEALATGEAALPVAGRIGDRQGVANLQANIGNVHLARGDIAKAEAA
                     YEESRTRFKSIGDHRGEAMALVAMGNVRFLSGEYQAAADCYRQSLKPRERMKDRYGLA
                     NALDNLGVAEYRIGRWEEARIHMLHAHTIRDELRDRPGQAESALNVGALYQVLGGTAL
                     ARKLFNDSLNLSVEMGDVQHEVKARLLLAGLDLWKGNDAAAAEHLTAANARSVAEPTL
                     KARAMTLEAMAGAGGDPVTAGERFVQAVAAADASGSLPERAAARLARARHLLGVDPAG
                     AAREAEGAMELIPPAVLPTLRLVACQVLQAAGQPRSDEAQILADLNGQLPPGQDPRPL
                     EEWARLPLP"
     gene            2108..2587
                     /locus_tag="VF720_03275"
     CDS             2108..2587
                     /locus_tag="VF720_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878401.1"
                     /translation="MHLLRSLLVATVLTVLTASVVGAQTRSVTTGGTATGSGVVKITI
                     EDFGKPGRHDLAPQSRLQAVYEVDVNVIAGWTCAQTTQAMYNALLAALPPEFIVAISK
                     TNPCILYIAKELHGTAPWGIEVIVTVPGQVIDVTDGVVPVQPTAWSAIKMRGWNRFN"
     gene            2630..>4860
                     /locus_tag="VF720_03280"
     CDS             2630..>4860
                     /locus_tag="VF720_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7878402.1"
                     /translation="MIAGDLSPYALIRRLGTGGMGEVHLALDERLGRRVALKFLKPEY
                     LGDASARQRFEFEARAAAILHHPDITTLFDFDAARGCISLEFVEGRTLEERLVDGPLT
                     VPEAIRIGIHVCRALDHAHGRNVVHRDIKPSNILLGDDGSIKLTDFGLAKVRDANFRT
                     ATGVVLGTAAYMCPEQIKGTGMDARGDLFALGVILHRAVSGRLPWNGEGVAVLYSILH
                     DTPPPLRSIDATIPAPFEAVVKRLLEKEPADRLASAAEVEGYLSEIEESLKRGAATSK
                     PLSPASAGSAPSGAGPAPRREPSGSEMAVIGPASAEAPPRPLIGREGELARLVAALED
                     TRLGHGRTLLLSSEAGGGKSRLMDEFRSRAEASGRLCLLGRCTPQAARNFQPFVEALE
                     EFARRTAGSRHRSEELTASHPELATVLPTLELLLDPGHDPALEPKSREQLWHLVDTLL
                     KRMARKEPLVLFLDDLHWADEGTLSLLHHVTMHHGGAHLLVVGAYRPEELVRAGEADP
                     PLLDLLRILSASDRFETLALGPLGEADTARLVRTQLGSSAPSGRWLEAIHHRAMGNPF
                     FALEMARLEHGEEAALPGTIADVLNRRLARLSGEEREILEMAAVEGEVFHTDVLEAGT
                     GLPRVTLLKRLRGLGQRHQLIAAVEEGHRFSHGLIREVLLDGIPAELRREYHAAVAGT
                     LLNGYSDRDDWAGRIGHHLFEGGRFADAVSYLTRAGFEARRLFLAERALEFLDQALGA
                     LERS"
BASE COUNT          753 a         1697 c         1663 g          747 t
ORIGIN      
        1 cgcacgacct gatcgagccg atcgccgagg ggtatcggtt cacccagggg ctgatccggg
       61 aggtgctgct gcgcgagctc cccgccgacc tcaggcagga gtgccactcg gtcgtggcgt
      121 cttacctgct gaagggatac gcggaccgat ccgactacgc cggaccgatt tcgggtcatc
      181 tctatcgcgc cgggcgattc ggcgaggcgc tgccgtatct ctggcgcgcg gccaccgaag
      241 cgcggcgtct cttcctgtac gaccgggcgc tgatctacct cgaccgggcg ctgacggcgc
      301 acaccgagtc gggcgccacc gacgtgcgcg gaacggaact cctcggagcc cgcgcggccg
      361 tgctcttgaa gctcggccgc cccgccgatg cccgtcagtc ggcccagtcg atgctgaccc
      421 aggcctcgga gggggccgat caccccgatc gatcggaagc cgaggagatc gtgggggagg
      481 ccgcgctcga ggaaggggat tacgacgctg ccgagaagca tctccagcag gcgttcgaac
      541 attaccagcg cgacgaatcg gccacgaccg gtctcccgcg gagccagcgg aagctggcct
      601 cgctctcgct ccggcgtggc gacttcgaca tggcgctcac gcgttatgcc gcggcccggg
      661 gtcggctcga ggaggcgggg gatcggcagg gagtggccga ggtgcgcatg gacctgggcg
      721 ccctcttcgc acgtcgggga gaattcgagg cggccctggc cgacctcgag gcggcactcg
      781 tcgagttccg cgcgctggga gacaagcacg gtgaggcgcg tacactgtcg ttcctggcca
      841 gcctctatcg gcgccagggg aggctcgagg aggcgcttgc caccggcgag gccgcgcttc
      901 cggtggccgg ccggatcggc gaccggcaag gcgtggccaa cctgcaggcc aacatcggca
      961 acgtgcatct ggcccgcggc gacatcgcga aggccgaggc ggcctacgag gagtcgcgga
     1021 cccgcttcaa gagcatcggg gaccaccgcg gcgaggccat ggccctcgtg gccatgggga
     1081 acgtccgctt tctctcgggc gagtaccagg ccgccgccga ctgctaccgg cagtcgctca
     1141 agccgcgcga gcggatgaaa gaccgttacg gcctggccaa cgcgctcgac aacctggggg
     1201 tggccgagta ccggatcggg cgatgggaag aagcgcgcat ccacatgctc catgcgcaca
     1261 ccatccgtga cgaattgcgc gaccggccgg ggcaggccga gtcggcgctg aacgttggag
     1321 cgctgtacca ggttctgggg ggtacggccc tggcacgaaa gcttttcaac gactccctga
     1381 acctttcggt ggagatgggg gacgtccagc atgaggtgaa ggcacgcctt ctcctggccg
     1441 gcctcgacct gtggaagggg aacgacgcgg cggccgctga gcatctgacc gcggccaatg
     1501 cccgttcggt ggccgaaccc accctgaaag cccgggccat gacgctcgag gcgatggccg
     1561 gagccggcgg cgatccggta acggcggggg aacgattcgt tcaggccgtt gcggcggccg
     1621 acgcctccgg ttcgctcccc gaacgtgcgg ccgcccggct ggcccgggcc cgccacctgc
     1681 ttggcgtgga cccggcgggg gcggcccggg aagctgaagg ggcaatggaa cttataccgc
     1741 ccgcagtcct gcccacgctg cggctggtgg cctgccaggt gctccaggcg gccgggcagc
     1801 cccgaagcga cgaagcgcag atcctggccg acctgaacgg ccagctcccc ccgggacagg
     1861 acccccgacc gcttgaggaa tgggcccgcc tcccgctgcc gtgaatcggc cgactggtcc
     1921 gaattcggca gtacggcgcg tttccttgca cgcaaccgac cgtccgttca ggtacacttc
     1981 cccgcattgg aaaccgtttt cctgggatat cggtagcggg cgccaaacgg cgccgcgacc
     2041 gcgctcaatt gtttggggac gattgacgcc gcttttctta gctccaggtg ttcccctcgg
     2101 aggcagaatg cacctcttga gatcactcct tgtcgccact gtcctcacgg tactgacggc
     2161 ctccgtcgtc ggcgcccaga cccgctccgt gacgaccggc ggcaccgcca cgggcagcgg
     2221 cgtcgtcaag atcaccatcg aggatttcgg gaaacccggg cgtcacgacc tggcgccgca
     2281 gagccggctc caggccgttt acgaggtgga cgtcaacgtc atcgccggct ggacctgcgc
     2341 gcagacgacc caggccatgt acaacgccct tctcgccgcg ctcccgcccg agttcatcgt
     2401 cgcgatctcg aagaccaacc cgtgcatcct gtacatcgcg aaggagctgc acggaaccgc
     2461 gccctggggt atcgaagtca tcgtgaccgt cccgggacag gtgatcgacg tcaccgatgg
     2521 cgtggtgccg gtgcaaccca ccgcctggag cgccatcaag atgcggggct ggaaccgctt
     2581 caactgaccg atcgaatttc gcccgcgatc atgaagtaat ctaccttcca tgatcgcggg
     2641 cgacctctct ccctacgcgc tcattcgacg ccttggaacg ggcggcatgg gcgaagtgca
     2701 cctggcgctg gacgaacgcc tcggccgccg ggtggccctc aagttcctga agcccgaata
     2761 cctgggcgac gcatcggccc gccagcgctt cgagttcgaa gcccgtgccg cggccatcct
     2821 ccaccatccc gacatcacca cgctcttcga tttcgacgcg gcccgcgggt gcatctcgct
     2881 cgagttcgtc gagggccgca ccctcgagga gcggctggtc gacggaccgc tcaccgtgcc
     2941 cgaggccatc cggatcggca tccatgtctg ccgggcgctc gaccatgccc acgggcggaa
     3001 cgtggttcat cgcgacatca agccgtcgaa catcctgctg ggcgacgacg gctcgatcaa
     3061 gctcaccgac ttcggcctgg ccaaggtgcg cgatgccaac ttccgcaccg ccaccggcgt
     3121 ggtgctcggc acggccgcct acatgtgccc cgagcagatc aaggggaccg gcatggacgc
     3181 gcggggcgat ctctttgccc tcggcgtgat cctccaccgg gcggtgagcg gccgactccc
     3241 ctggaacggc gagggagtgg ccgtgctgta ctccatcctt cacgacacgc cgccgccgct
     3301 tcgctcgatc gatgccacca tcccggcgcc cttcgaggcg gtggtgaagc ggctgctcga
     3361 gaaggagccg gccgaccggc tggcctcggc tgccgaggtg gagggttatc tctccgagat
     3421 cgaggagtcg ctcaagcggg gggccgccac ctcgaaaccg ttgtcgcccg cctcggcggg
     3481 ttccgccccg agcggcgcgg gcccggctcc gcgccgggag ccatccggtt cggagatggc
     3541 ggtcatcggc cccgcatcgg cggaagcccc cccccgccct ctcatcggcc gcgaaggcga
     3601 gctggcccgc ctcgtggccg cgctcgagga tacgcgcctc ggacacgggc gcaccctcct
     3661 cctgtcgtcg gaagccggcg gcggcaagag ccggctgatg gacgaattcc gatcccgtgc
     3721 cgaggcctcg ggccggctct gcctccttgg ccgctgcacg ccccaggcgg cccgcaactt
     3781 ccagccgttc gtggaggcgc tcgaggaatt tgcccgccgg acggccggat cgcggcatcg
     3841 cagcgaggag ctcaccgcct cgcatcccga gctggccacg gtgctgccga cgctggagct
     3901 gttgctcgat ccgggccatg atccggcgct cgagccgaag agccgggagc agctctggca
     3961 cctggtggat acgctcctca agcggatggc ccggaaggaa ccgctggtgc tctttctcga
     4021 cgacctgcac tgggccgacg aaggaacgct ctcgctcctg caccacgtga cgatgcacca
     4081 cggcggcgcg cacctcctgg tggtgggagc ctaccggccc gaggagctgg tgcgggccgg
     4141 cgaggccgat ccgccgttgc tcgaccttct ccggattctc tcggcctccg atcgattcga
     4201 gacgctggcg ctggggcccc ttggcgaggc cgataccgcc aggctcgtgc gcacgcagct
     4261 cggctcctcg gcgccgagcg gacgctggct cgaagccatc caccatcgcg ccatgggaaa
     4321 tccgttcttt gcgctggaaa tggccaggct cgaacatggc gaagaggccg cgctccccgg
     4381 aacgatcgcc gatgtcctga accggcggct ggcgaggctt tccggcgagg aacgggagat
     4441 ccttgagatg gccgccgtcg aaggagaggt gttccacacc gacgtgctcg aggcggggac
     4501 cggccttccg cgcgtcacgc tgttgaaacg actgcgcggg ctcggccagc gccatcaact
     4561 gatcgcggcc gtggaggagg ggcaccgctt ttcgcacggg ctgatccgcg aggtgctgct
     4621 cgacgggatc cccgccgagt tgaggcggga gtaccacgcc gcggtggcgg gaacgcttct
     4681 caacggctat tccgaccggg acgactgggc ggggcggatc ggtcaccacc tcttcgaggg
     4741 tgggcgcttc gccgacgcgg tgtcgtacct gacgcgggcc ggtttcgagg cgcggcgcct
     4801 cttcctcgcc gagcgtgcgc tcgaattcct cgaccaggcg ctcggcgcgc tcgaacgttc
//
LOCUS       DASVOD010000161         5089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_323159, whole genome shotgun sequence.
ACCESSION   DASVOD010000161 DASVOD010000000
VERSION     DASVOD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323159"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF720_03285"
     CDS             <1..192
                     /locus_tag="VF720_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878403.1"
                     /translation="EVSLYDVAGRRVRTIVAGDFDAGEREAAWDGRDDTGRPVANGVY
                     FLAATSGGTSTRIKVGVVR"
     gene            194..1306
                     /locus_tag="VF720_03290"
     CDS             194..1306
                     /locus_tag="VF720_03290"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7878404.1"
                     /translation="MNRFALLLPLLIVPILASGKADAATLLALLDTGEMAASEDGGSS
                     WSFRAAIPVPDAAALVAGATPEELHLVSRSGLLYRSGNGGDTWTAVATLPTNDVVDLL
                     PAGGGILVISASGVIHRSSGGNAPFIAWGTMTGQDLVSMARRQDGRLAAITRSGVVFR
                     SDDDGQSWELNGFFPVSDAVAIRADGPDFVAITGTGLAWRSTDGGRTWNAVGSTSQVT
                     TAGMTVSGSSLVAVTEEGLVATSADGSQWSWVGTVNQVRVVAVANDAPQVVGVPGPGS
                     GPVAVLQLSSPFPNPPPAVGLPVYVDVTLGSRESVRLDIVDVMGRIVRTWPETPQGPG
                     TVRLTARPAGLPAGSYRLRVSTARGAAASRPIVIPG"
     gene            complement(1317..3602)
                     /locus_tag="VF720_03295"
     CDS             complement(1317..3602)
                     /locus_tag="VF720_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX7878405.1"
                     /translation="MPDLSQLPATLYLMVGALAVLLGVVIARENARERIHRVTAALLI
                     LVGLKAAYEGLIYAAAPATMVAAPVDPVLRALNYFWEFFFPTLVLFASIFPAEHRWMS
                     RIRFGPVLLFLPHTFHFLLLMYLGILGRNDLGLESLAPEGGWLRGIFRLSETLFGILL
                     SLHLPFFSLVNICYVTGATWLLYRSWRATSNPRLATQLRIITIGVGSCVGLYMVAEPM
                     PVLLGIEMAPWIRQGLVVGALTICVGAIAWSIVRYRFLDTQLLARRSILYALVTALLV
                     GAYLTLVRQLQGLLARALGRELTVIEPVFLVLALILFQPVLQRLEEIIDSWFLRDRHD
                     HRNILRGLSRDIISILDRKELTRKIVSTLAEGVMARQSILIDLEAPQGERVTAAEAVL
                     EEAEKLEDDVILAELRLLPLDEDFVHRDQVAGRLQAWGTPVIDLLYQLRVRYLLPIRH
                     GDRLLGLLLLGRKVTNVSYTGEEVSLLSTLANQVGVALQNSALYRQSLEKAVLEEELS
                     LARQIQERLLPAELPQVAPFLVSAINVPSKTVGGDFYDFVELEDGRVLFAIADVAGKG
                     VPAALLTSMLQASLRTQMHVMTTPSRILANLNDLTYRFTAADQFATFFLGVLDPVTRT
                     LVYANAGHNFPMRIVAGGAVEWLSEGGLLLGAFPGIQWGETTLHLGPGDCLVFYTDGV
                     TEARPNGQDEEYGERRFLEQLERLRHVAPRRMMDAILEDVAAYAGDEQADDITLIIIR
                     IDGAHEATLSSDDARALAGHL"
     gene            complement(3625..>5089)
                     /locus_tag="VF720_03300"
     CDS             complement(3625..>5089)
                     /locus_tag="VF720_03300"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7878406.1"
                     /translation="GELERAFDYPERVARVSVADVQAAARRYFGVSKATTVLYRPHGT
                     PLVNDPAALEARLSAATRGVAGAAPMAAMSTAAFQVHRHRLPNGLTVLTESIPGLPIV
                     AMTMQCRCGSGEETKGNNGITHLMQQLRLKGAGGRDARMLASEIESMGGHIRPFAGRD
                     TSGVSMSVLRSRLDDLLPLFADVARRPDFGPDEIERERAKMLADLAAMRDSSLSFTMQ
                     AFNEALFAGHPYGLAPLGNVDSVPTLNRGDLLEWHVTHLAPERMVVAVVGDIADDEAL
                     ERVERWFGDLPPGRPLGVVPEVDPTPVSRKVVLTKDVAQSVIVLGCPGPAHDSPERYA
                     LDVLMSVLSGMGNRLFYELRDRQHLCYFTGSFASLLRAGGVVGAYIGTRPENETQAID
                     GLLSELERIRHEPPTDEEMMRAKNTIAGGYVIDLQRRAARASLLAQDEVSGLGYEEAL
                     GYLDRIRTVTAGEVRDVAARWFQLQSTTLTILKPPAA"
BASE COUNT          849 a         1649 c         1828 g          763 t
ORIGIN      
        1 gaggtctcgc tgtacgacgt tgcgggacga cgcgtccgga ccatcgtcgc gggggacttc
       61 gacgcgggcg agagggaggc cgcctgggac ggccgcgacg acacggggcg tccggtggcc
      121 aacggcgtct acttcctggc cgcgacgagc ggggggacga gcacccgcat caaggtcggc
      181 gtggtgcggt gacatgaacc ggttcgcgct tctgctgccc ctgctcatcg tcccgatcct
      241 ggcatcggga aaggccgatg ccgccacgct cctggcgctc ctggacaccg gcgagatggc
      301 cgcgtccgag gatggcggct cgagctggag tttccgtgcc gcaatcccgg tgccggatgc
      361 ggccgcgctc gtcgcggggg caactcccga ggaactccat ctcgtgtcac ggtcggggct
      421 cctgtaccgg tccgggaacg gcggcgatac atggacggcc gttgccacgc tgccgacgaa
      481 tgatgtcgtt gacctccttc cggcaggagg tgggatcctg gtgatttccg cgtccggcgt
      541 gatccatcga tcgagcggcg gcaacgcgcc cttcatcgcg tggggaacca tgacgggcca
      601 ggacctcgtc tcgatggcgc gccggcagga tgggcggctc gccgcgatca cccgaagcgg
      661 agtggtcttc cgaagcgatg acgacggaca atcctgggag ttgaacggct tctttcccgt
      721 gagcgatgcc gtggcgatcc gcgcggacgg accggacttc gtggccatca ccggaaccgg
      781 ccttgcgtgg cgatcgacgg atggcggcag aacctggaac gcggttggtt cgacctcgca
      841 ggtgacgacg gccggaatga cggtctccgg ttcttccctg gtggccgtca ccgaggaggg
      901 gctggtggcc acgagcgccg atggctccca atggagctgg gtgggcacgg tgaaccaggt
      961 ccgcgtcgtg gccgtggcca atgacgcacc gcaggtcgtc ggtgtgcccg gcccgggttc
     1021 cggcccggtg gccgtgcttc aactttcttc acccttcccc aacccgccgc ccgcggtggg
     1081 actaccggtg tacgtggatg tcacgctcgg cagccgggaa tcggtgaggc tcgacatcgt
     1141 cgacgtcatg ggaaggatcg tccgaacgtg gcccgaaacg ccacagggcc cggggaccgt
     1201 gcggctgacg gcgcgtcctg ccgggcttcc cgccggaagt tatcggctgc gtgtcagcac
     1261 ggcgcgcggc gcggcggcca gccgaccgat cgtgatcccg gggtgaaacg gccgcgtcag
     1321 aggtggccgg ccagggcccg ggcgtcgtcg ctgctcaacg tggcctcgtg cgcgccgtca
     1381 atccggatga tgatgagcgt gatgtcgtcg gcctgctcat ctcccgcgta agccgccacg
     1441 tcctcgagaa tggcatccat catccgccgc ggcgccacgt gccggaggcg ctcgagctgc
     1501 tcgaggaagc gccgctcgcc gtactcctcg tcctgcccgt tgggccgcgc ctcggtcacg
     1561 ccgtcggtgt agaagacgag gcaatccccg gggccgaggt gaagagttgt ctcgccccac
     1621 tggatgccgg ggaatgcgcc gagcagcagc cctccctccg agagccattc caccgcgcca
     1681 ccggccacga tgcgcatggg gaaattgtgc ccggcgttgg cgtacaccag ggtccgagtc
     1741 acgggatcga ggacgccgag gaaaaacgtg gcgaactggt cggcggccgt gaagcggtag
     1801 gtgaggtcgt tcaggttggc caggatgcgg ctcggcgtgg tcatcacgtg catctgcgtg
     1861 cggagcgagg cctgcagcat cgacgtgagg agcgccgccg gcaccccctt gccggccacg
     1921 tcggcgatgg cgaacaacac gcggccgtcc tccagttcga cgaagtcgta gaagtcgccg
     1981 cccaccgtct tgctggggac gttgatggcc gacacgagga aaggcgccac ctggggcagc
     2041 tccgcgggaa ggaggcgttc ctggatctgc cgcgccagcg acaattcttc ctcgagcacg
     2101 gccttctcga ggctctgccg gtagagggcc gagttctgga gcgccacgcc cacctggttg
     2161 gccagcgtgg aaagaaggct cacttcctcg ccggtgtacg acacgttcgt gaccttgcgg
     2221 ccgaggagga gcaggccgag caggcgatcg ccgtggcgga tcggcaggag gtaacgcacc
     2281 cggagctggt agagaagatc gatgaccggc gtcccccagg cctggagccg gccggccacc
     2341 tggtcgcggt ggacgaaatc ctcatccaga ggcaggagcc gcagttcggc caggatcacg
     2401 tcgtcttcca gtttctcggc ttcctcgagg acggcctcgg cggccgtcac gcgctcgccc
     2461 tgcggtgcct cgaggtcgat gaggatcgac tggcgggcca tgactccctc ggcgagcgtg
     2521 gacacgatct tcctcgtcag ctccttccgg tcgaggatcg agatgatgtc gcgggagagg
     2581 ccgcgcagga tgttgcgatg gtcgtgccgg tcgcgcagga accagctgtc gatgatctcc
     2641 tcgagccgct gcagcaccgg ctggaagagg atgagcgcca ggacgaggaa gaccggctcg
     2701 atgacggtga gctcgcgccc gagcgcccgg gcgagcagcc cctggagctg ccggaccagc
     2761 gtgaggtagg cgccgaccag gagtgcggtg accagcgcgt acaggatgga gcggcgggcc
     2821 aggagctgcg tgtcgaggaa gcggtaacgc acgatcgacc aggcgatggc accgacgcag
     2881 atcgtgagcg cgcccaccac cagcccctgc cggatccagg gggccatctc gatgccgagg
     2941 aggaccggca tcggctcggc caccatgtag agcccgacgc acgagccgac cccgatagtg
     3001 atgatccgga gctgggtggc gaggcgagga ttggacgtgg cgcgccacga gcggtagagg
     3061 agccaggtgg caccggtcac gtagcagatg ttgaccagcg agaagaaggg aaggtgcagc
     3121 gagaggagga tgccgaagag cgtttcgctc agccggaaaa tcccgcgaag ccatcctccc
     3181 tcgggcgcca gtgactcgag gccgaggtca ttccgcccga ggatgccgag atacatgagc
     3241 agcaggaaat ggaacgtatg cgggaggaac aggagaacgg gcccgaaacg gattcggctc
     3301 atccagcggt gctcggccgg gaagatcgag gcgaagagca ccagcgtcgg gaagaagaat
     3361 tcccagaagt agttcagcgc ccggaggacg ggatcgaccg gcgcggcaac catcgtggcc
     3421 ggcgccgcgg catagatcag cccttcgtag gccgccttga ggccgacgag gatgagcagc
     3481 gcggccgtga cccggtgaat ccgctcgcgg gcgttctccc gggcgatgac gacgcccagc
     3541 agcacggcca gggccccgac catgaggtac agggtggccg ggagctgcga gagatcgggc
     3601 acgggcggtc tcctccccgg tagctcaggc ggccggcggt ttcaggatgg tcagggtggt
     3661 tgactggagc tggaaccacc gggcggccac gtcacgaacc tctccggcgg tcacggtgcg
     3721 gatgcgatcg aggtagccga gcgcctcctc gtatcccagc cccgaaacct cgtcctgggc
     3781 caggagcgac gcccgggcgg cccgccgctg caggtcgatg acgtatccgc cggcgatcgt
     3841 gtttttggcc ctcatcatct cttcgtcggt cgggggctcg tgacggatcc tttccagttc
     3901 ggacaggagg ccgtcgatag cctgcgtttc gttttcggga cgggttccga tgtacgcccc
     3961 gaccaccccc ccggcccgga gaaggctggc gaacgacccg gtgaaatagc agagatgctg
     4021 gcgatcccgg agctcgtaga agagccggtt ccccattccg gaaaggaccg acatcaggac
     4081 atccagcgcg taccgctcgg ggctgtcgtg cgccgggccg gggcaaccca ggacgatgac
     4141 cgactgcgcc acgtccttgg tgaggaccac ctttcgcgaa accggcgtcg ggtcgacctc
     4201 ggggaccacg cccagcgggc gacccggggg gaggtcgccg aaccatcgtt ccacacgctc
     4261 cagcgcctcg tcgtcggcga tgtcacccac cacggccact accatacgtt cgggcgcgag
     4321 atgggttaca tgccattcga ggagatcgcc acggttcagc gtgggcaccg aatcgacgtt
     4381 gccgagcggc gccaggccat agggatgccc cgcgaagagc gcttcgttga aggcctgcat
     4441 cgtgaacgac agggaggaat cgcgcatggc cgcgaggtcc gccagcatct tggctcgttc
     4501 ccgctcgatc tcgtccggcc cgaagtccgg ccgtcgcgcc acgtcggcga agagcggcag
     4561 gaggtcgtcg aggcgcgagc gaagaaccga catcgagacg cccgaggtat cgcgcccggc
     4621 gaaggggcgg atgtgccccc ccatcgactc gatctcggag gcaagcatcc gggcatcccg
     4681 cccaccggcg cccttgaggc ggagctgctg catgagatgg gtgatgccgt tgttcccctt
     4741 cgtctcctcc ccgctgccgc accggcactg catcgtcatg gccacgatcg gaagcccggg
     4801 gatcgattcg gtgagcacgg tgagcccgtt cggcagccgg tggcgatgca cctggaacgc
     4861 ggccgtcgac atggcggcca tcggggccgc tccggccacc ccacgggtcg cggcggagag
     4921 ccgggcctcg agcgccgccg ggtcgttcac gaggggagtg ccgtgcgggc ggtaaagcac
     4981 cgtggtggcc ttcgacacgc cgaagtagcg ccgcgcggcg gcctgcacgt cggccaccga
     5041 gaccctggcc acccgctcgg ggtaatcgaa cgcccgctcc agttcaccg
//
LOCUS       DASVOD010000162         1612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_11351, whole genome shotgun sequence.
ACCESSION   DASVOD010000162 DASVOD010000000
VERSION     DASVOD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11351"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VF720_03305"
     CDS             complement(<1..528)
                     /locus_tag="VF720_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX7878407.1"
                     /translation="MTCRLRGPAAALVVLLLAPAALNAAAPPNYYNSVNADTPALLRS
                     TLHNVIKDHTRYPYTSSGTDTWTILEIADQNPANSGQSIDLYRNRALTKFGGGTGPYN
                     REHTWPNSYGFPNDGGANYPYTDCHHLFLCDVGYNSDRANKPYGAAAAGATERVTDVN
                     GGQGGGSGIFPGNSNW"
     gene            complement(648..1457)
                     /locus_tag="VF720_03310"
     CDS             complement(648..1457)
                     /locus_tag="VF720_03310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7878408.1"
                     /translation="MKARHMLMSCCLAALVGCASPAKSPPTLADYEPIDLSHPFNSQT
                     LYWPTSPTNFALQSLFDGQTPGGWHYSANSICTPEHGGTHLDAPIHFAAGGQTADQVP
                     LERLIGPAVVIDIAAKAAANVDYLLSVDDVTQFEKSHGAIAPGTIVLVRTGWDARWPD
                     RKSYFGDDTPGDASNLHFPSFGPEAARLLVEQRKVAAIGIDVASIDNGASRDFQVHRV
                     AAAANVPGFENLAHLDRLPPTGAMVVALPMKIEGGSGGPLRAVALVPRPSR"
BASE COUNT          246 a          483 c          606 g          277 t
ORIGIN      
        1 ccagttggag ttgcccggaa agatgcccga cccgcccccc tgcccgccgt tcacgtcggt
       61 gacgcgctcg gtggcgccgg ccgccgcggc gccgtacggc ttgttggcgc gatcactgtt
      121 gtagccgacg tcgcacagga agaggtgatg gcaatcggtg tagggatagt tcgcgccgcc
      181 gtcgttggga aagccgtagc tgttgggcca ggtgtgctcg cggttgtagg ggccggtgcc
      241 gccgccgaac ttggtcagcg cgcggttgcg gtacaggtcg atgctctgcc cggagttggc
      301 cggattctgg tcggcgatct cgaggatcgt ccaggtgtcg gtgccggacg acgtgtaggg
      361 gtagcgcgtg tgatccttga tgacattgtg cagcgtgctg cggagcaggg ccggcgtatc
      421 ggcgttgacc gagttgtagt agttgggcgg cgcggcggcg ttgagggcgg cgggagcgag
      481 cagcagcacg acgagggcgg cggccgggcc acgaagccgg caagtcattg aggccattaa
      541 cgttctccga tgtgttgcca aggacttgcg gacaccaatg tgccggccgc gatgcggatg
      601 gacaattcta atggatggcg cccgaatcgc cacctgaaat ctggggccta ccgactcgga
      661 cggggcacga gcgccacggc ccggagcggc ccgcccgagc ccccctcgat cttcatcggc
      721 agggcaacga ccatggcccc ggtggggggc aggcgatcga gatgcgccag gttctcgaaa
      781 ccgggcacgt tggccgccgc ggcgacgcga tgcacctgga agtccctgga agcgccgttg
      841 tcgatcgagg ccacgtcgat gccgatggcg gccaccttgc gttgctcgac gagcaggcgc
      901 gcggcctccg gcccgaagct cgggaagtgg aggttcgagg catcgcccgg cgtgtcgtcg
      961 ccgaagtacg acttgcgatc gggccagcgg gcatcccacc ccgtgcggac gagcacgatc
     1021 gttccgggtg cgatcgctcc gtgcgacttc tcgaattgtg tcacgtcgtc cacgctcagg
     1081 aggtagtcca cgttggccgc cgccttggcc gcgatgtcga tgaccaccgc gggaccgatg
     1141 aggcgctcga gcggcacctg gtcggccgtc tggccgccgg ccgcgaagtg gatgggtgcg
     1201 tcgaggtgcg tgccgccatg ctccggcgtg cagatcgagt tggcggagta gtgccagccg
     1261 cccggcgtct ggccgtcgaa gagcgattgc agtgcgaagt tggtcggcga ggtgggccag
     1321 tagagggttt gggagttgaa cgggtggctc aggtcgatcg gttcgtagtc ggcgagggtt
     1381 ggcggtgact tcgccggtga cgcgcatccg accagcgcgg cgaggcaaca cgacatgagc
     1441 atgtgacgtg ccttcatgac tcgatcctcc aatgcggtgc tggcaccgac gtcatccagc
     1501 ctgagatttg tccagcagtt gtgcgagctc cgaatccgga ccgacaccga cagcggccgc
     1561 gatgtcccgg gcctccgtga gcgcggactc ggcgaggggg gcattgccgg cg
//
LOCUS       DASVOD010000163        10829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_584466, whole genome shotgun sequence.
ACCESSION   DASVOD010000163 DASVOD010000000
VERSION     DASVOD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10829
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584466"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF720_03315"
     CDS             <1..309
                     /locus_tag="VF720_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX7878409.1"
                     /translation="ERGGRVVAVGTTVVRTLESAVRTDQSGRHRLESGAGWADLFIYD
                     PFTFRAVDALITNFHLPRSTLLMLVSAFAGLEATRRAYRAAIREGYRFYSYGDSSLYL
                     "
     gene            331..861
                     /locus_tag="VF720_03320"
     CDS             331..861
                     /locus_tag="VF720_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HEX7878410.1"
                     /translation="MDQDLRYPVGKVQFKDGDVTPADRKGWIAAIAETPANMRKAVSG
                     LSDVQLDTPYRPEGWTVRQVVHHVPESHMNAFIRIKWSMTEENPTIKGYDEKAWANLP
                     DARLPIAMSLDLLEALHVRVVALLNALGDGDFSRTYFHPESQRQFTLDQLLQIYAWHG
                     RHHTAHITSLRERKGW"
     gene            866..1147
                     /locus_tag="VF720_03325"
     CDS             866..1147
                     /locus_tag="VF720_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX7878411.1"
                     /translation="MTTAVILLNCERGQVNAVAQALAAIDGITEVYSVAGRWDLVAMI
                     RVRRNEDLASLVTAQMLKVPGIRETETLIAFQAFSKHDLEAAFSLGGES"
     gene            1168..2292
                     /gene="tgt"
                     /locus_tag="VF720_03330"
     CDS             1168..2292
                     /gene="tgt"
                     /locus_tag="VF720_03330"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX7878412.1"
                     /translation="MFRFQLERTEGAARLGAFENARGRVETPVFMPVGTQASVKTLSA
                     PELEGAGAGIILANTYHLYLRPGHALIRELGGLHRFSAWNRLFLTDSGGFQVYSLADL
                     NRITDQGVRFQSHLDGSYHEFTPELSMQIQTDLGSDIVMVFDQCTTWPCAPEDSLVAL
                     ERTTRWALRSREAFDRRDALPGSDQALFGIVQGSVFPDQRRRSLEELMAIGFDGYAVG
                     GLAVGEPKSAMWETLEALEPHLPKDRPRYLMGVGTPEDLIEGVRRGIDMFDCVLPTRN
                     ARKGTVFTRDGRLVVKNSSFARDERPLDPDCNCLTCTRYSRAYLRHLFATGELLGPRL
                     ASLHSVYFYLALMTELRTALREGRFDSWSRRFLARYRGTD"
     gene            2354..2707
                     /gene="yajC"
                     /locus_tag="VF720_03335"
     CDS             2354..2707
                     /gene="yajC"
                     /locus_tag="VF720_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX7878413.1"
                     /translation="MLLPATLLAFMPLTLPLMAPAPVGGEAPNPLIQMLPIVLIFVIF
                     YFLLIRPQQAQQKKHQEMLKNVKKGDRIITSGGIYGRVVGVGDNDVTVQIAKDVEVQL
                     QKGSITAVVAAEGGK"
     gene            2713..3231
                     /gene="def"
                     /locus_tag="VF720_03340"
     CDS             2713..3231
                     /gene="def"
                     /locus_tag="VF720_03340"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX7878414.1"
                     /translation="MAREELHLRLYGDPVLRQKAADITVFDKSLAKRAESMFEIMYEN
                     DGIGLAGNQVGLLERLIVLDVSLDDENRVRMALANPRILTRAGEDTAEEGCLSIPDVR
                     ADVTRPDVLTVEAEGLDGKTIRFEAEGLLARAIQHEIDHLDGVLFIDRISATRRKLLE
                     GRLKEIARESAS"
     gene            3264..4280
                     /gene="fmt"
                     /locus_tag="VF720_03345"
     CDS             3264..4280
                     /gene="fmt"
                     /locus_tag="VF720_03345"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEX7878415.1"
                     /translation="MRVVFMGTPVFSVPCLVAMVEAGHTVPLVVTQPDRPKGRGRELS
                     PTPVKQAALDRNIAVFQPERPNRDDAVATIEAARPDVLVVVAYGAILKARLLGLAPKG
                     AVNVHASLLPRHRGMSPIHRAILDGDEETGITTMFMDEGVDTGDMILIETTPIAPNET
                     TGQLHDRLAQMGARLLVDTLARIEAGTAPRTPQPPLGASYAGRIDKIDGVIDWSRNAA
                     QVHNRIRAMTPAPGATGSLEGQPLLVAGSLLVDESVQDAIPHGDTTRTPGGEPAIPGT
                     VLAVMRDALRVATGQGAIDIHEVRPAGGRTMSASAYARGRGIAPGSRFYAPPASPGAP
                     GAAR"
     gene            4277..5641
                     /gene="rsmB"
                     /locus_tag="VF720_03350"
     CDS             4277..5641
                     /gene="rsmB"
                     /locus_tag="VF720_03350"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEX7878416.1"
                     /translation="MNRPYRPRREAPPKTPREVALEALYAVDRRHAFSDRLLHKLLRD
                     HPMDPRDAALVTNIVRGTLRWQGRIDGLLNHYIKIGLDALPPMIQNILRMGVFQMLYM
                     DRIPPSAAVDESVKLAKKFGHPGTAGLVNAVLRKMVTEKDRLPEPRLGDNPAPALATI
                     YSHPQWMIDRWLLRFGLDETKELLEANNSPPPVTIRINPNKTTREEFLKRLHDLSLTA
                     EVGEHHKSTIKVHGDFAPASVSSFHQGLFTVQDESESMVVDLLDPRPGERIVDLCAAP
                     GGKTSQIAELIGPGGTVIAVEGQKGRLKPLSENKLRMSMDNAQLVLADGRRLALRKPV
                     DKVLIDAPCSGLGVLAKRADARWRKTEAGIRQIAELQAQLLEAGAALVKPGGVLVYSV
                     CSMEPEEGDQQVRAFQKRHPEWRIDDAAQYVPADVVTDRMLQVFPHRHHMDGAFAARL
                     VKEG"
     gene            5641..6414
                     /locus_tag="VF720_03355"
     CDS             5641..6414
                     /locus_tag="VF720_03355"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX7878417.1"
                     /translation="MNTPPERIDAPGPRSRPQLAGLIQIALGLVAALVVTVILFNNVV
                     MPGFVGRGNVTRVPDVTGKELTQAQSLLTKAGLTPGETRQRNDPKPAGTVIRQQPQAG
                     NSVKDGRPIILTVSLGEPGRSVPTLAQESIRNAELALGDVDLVLGQVTRAPTDNIPTD
                     LVVASQPPAGTTLPRGSTVDILVSAGTSRRSAGYVMPNLVGRETRPTRDRLLDAGFKV
                     TVDEKSFGFFRVGNTHVTEQDPEPGSRVMPWDTIWLKGE"
     gene            6419..7144
                     /gene="rpe"
                     /locus_tag="VF720_03360"
     CDS             6419..7144
                     /gene="rpe"
                     /locus_tag="VF720_03360"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006557241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX7878418.1"
                     /translation="MIPDRPLIAPSILSADFTQLAEQIHLMETAGAQLLHLDVMDGHF
                     VPNITFGPMVVEAVNRITPLFLDVHLMIAEPQKYAEAFVKAGADRLLVQIETQADPVP
                     LLDRIRGLGAEAGLVLNPETPFEAVEPHLAHIDCLLVMSVHPGFGGQAFMAEVLPKVQ
                     KARALREMHGWDWPIEIDGGIDSATVVPAVEAGTSILVAGNAIYKYPDPSDAFTRLRL
                     TAELAGESSGRPRGLIRTDEGFL"
     gene            7190..7459
                     /locus_tag="VF720_03365"
     CDS             7190..7459
                     /locus_tag="VF720_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878419.1"
                     /translation="MTFPGFDARPLALALAVGIAFVGLPLLGVVGPSDVRSAEYVYET
                     ADSLHPHIRYDDGSVSVNDRCAVRMTKLSRSMRPIYVNGKPIGFC"
     gene            7496..7816
                     /locus_tag="VF720_03370"
     CDS             7496..7816
                     /locus_tag="VF720_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878420.1"
                     /translation="MYLKARGIDVRDYFDPDIVAVLDMEHRSAVNYELYFFGNSQSKA
                     RFDKDILKHCGVVTDPIIRTRFRPSHLSPHEDYMGRRYYFLTHGTQAEFAAHPDSFAV
                     RQGM"
     gene            7864..10200
                     /locus_tag="VF720_03375"
     CDS             7864..10200
                     /locus_tag="VF720_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878421.1"
                     /translation="MTRIRIGTLALATVLATAFSAVALVMGDSVTPLSPADVTPPTVT
                     VMSPNGGESWTAATTHSVTWTATDDVNVSTVDILYEDAPGAGWTLIARDLENTGSWIW
                     PVHNTPGTGVRVQVRARDTSANVGADSSNAVFTILIEPGGRLPTTLRDFRMPGTQPLA
                     INNFNRSSSCNGCHIGFNAATEPGFGFKGSMMAQAGRDPLFYACMAIANQDARSSGDL
                     CIRCHSPFGWMEGRSNPTDGSALTAADRDGVSCDHCHRMVDPVYQLGISPVEDAAVLA
                     AMPVADRPTGAHGGQYVDDPNVRKRGPYADANPPHAKLASNFHRSSDLCATCHDLSNP
                     VYTRTGDDDYAPGSFNSAPGMVASDTHMPIERTFSEWAASNFPSGVYAPEFAGVKPGG
                     IVSTCQDCHMADVVGKGCSSGSTPVRSDLGFHDFTGGNSWMPGVIGALYPSETDPAAL
                     AAAATRARGMLAKSAVVDVGLSAATGNLVATVTVTNRTGHKLPTGYPEGRRMWLNVVA
                     YDGVNTKVYESGAYNASTGVLTEDAAARVYEVQAGLSAPIATQASLTAGPSFHFVLND
                     TIYKDNRIPPAGFTNAEFILFGGRPVEPGHPAPRYADGQNWDTASYTLPSTTRKVVAT
                     LRYQTTSREYVEFLRDANVTNTAGQVMYDAWVAHGKSTPVTMAADSMVIGATGVPDEV
                     ALTAPTTIGPLSAMSNPFRELLALSFELSGPARVSWQVFDVRGRRIAAGEPGMLGGGA
                     HRLDWDGRMEEGLAPAGIYWVRVTAGAEEQVLRVVRVR"
     gene            complement(10219..>10829)
                     /locus_tag="VF720_03380"
     CDS             complement(10219..>10829)
                     /locus_tag="VF720_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7878422.1"
                     /translation="RADKALEVAGLVADGKRDYGTWSRGMRQRLKLGWALSGDAALVL
                     LDEPFLGVDPPSRRQLTELIRALAAEGRTVMLTSHVLSEIETLTSRVAVLAHGRLLGF
                     GQLEDLLRSLRDRHPHRVHLDVVGDPRPLGRLLLDRPDTLEVRITGPSSLSFLTSGPD
                     TLYPELPALVTASGAAVWRLQAPDDTLEAVFRHVTEAGTRRL"
BASE COUNT         1877 a         3740 c         3497 g         1715 t
ORIGIN      
        1 gagcggggtg ggcgcgtggt ggccgtggga accaccgtcg tccgcacgct cgaatcggcg
       61 gtccggacgg accaatcggg gcgacatcgg ctggaaagcg gtgcggggtg ggccgacctt
      121 ttcatctacg acccgttcac cttccgcgcg gtggatgccc tgatcacgaa cttccacctg
      181 ccgcgctcca cgctcctcat gctggtatcc gctttcgccg ggctggaagc cacgcgacgg
      241 gcctatcgcg cggccatccg ggaggggtat cgtttctact cctacggcga ttcgtcgctc
      301 tacctctgac cgcccgcaac aggagtttcg atggaccagg acctccgcta tcccgtcggc
      361 aaggtgcagt tcaaggatgg ggacgtcacg cccgccgatc gcaagggttg gatcgccgcc
      421 atcgccgaaa cgcccgccaa catgcgaaag gcggtgtcgg ggctgagcga tgtgcagctc
      481 gacacgcctt atcgccccga agggtggacg gtgcgtcagg tcgtgcacca cgtgccggaa
      541 agccacatga acgcgttcat ccgaatcaag tggtccatga ccgaggagaa tcccacgatc
      601 aaggggtacg acgagaaggc ctgggccaac cttcccgatg cccgtctccc gatcgcgatg
      661 tcgctcgacc tgctcgaggc gctgcacgtg cgcgtcgtgg cgctcctgaa tgcgctgggc
      721 gatggcgatt tctcccgcac ctacttccat cctgagagcc agcgtcagtt cacgctcgac
      781 cagttgctcc agatctacgc ctggcacggc cggcatcaca ccgcgcacat cacgagcctg
      841 cgcgagcgga aaggttggtg accggatgac caccgccgtg attcttctca actgcgagcg
      901 gggccaggtg aatgccgtgg cccaggcact ggccgccatc gacggcatca ccgaggtcta
      961 ctccgtggcc ggacgttggg acctggtggc gatgatccgg gtgcgcagga acgaggacct
     1021 ggcaagcctg gtcaccgcgc agatgctcaa ggtgcccggc atccgggaaa cggaaacgct
     1081 gatcgccttc caggctttct cgaagcatga cctcgaggcc gcattcagcc tgggcgggga
     1141 gagctagaac cgcccccgcg gtcccgcatg ttccgattcc agctggaaag gaccgagggg
     1201 gccgcgcgac tcggcgcctt tgaaaatgcc cgcggccgcg tggaaacgcc cgtcttcatg
     1261 ccggtcggca cgcaggcttc ggtcaagacg ctctcagctc ccgagctcga aggggccggc
     1321 gcggggatca tcctcgccaa cacgtatcac ctgtacctgc gccccggcca cgcgctcatc
     1381 cgggagctgg gcgggctcca ccggttcagc gcctggaatc gcctcttcct caccgattcc
     1441 ggcggcttcc aggtgtacag cctggccgac ctcaacagga tcaccgacca gggggtgcgc
     1501 ttccagagcc atctcgacgg ctcgtaccac gagttcacgc ccgagctgtc gatgcagatc
     1561 cagaccgacc tgggctcgga catcgtgatg gtgttcgacc agtgcaccac ctggccctgc
     1621 gcgccggaag actccctggt ggcgctcgag cgtacgacgc gctgggcgct ccgctcgcga
     1681 gaggcgttcg accggcgcga cgccctcccc ggcagcgacc aggccctgtt cggcatcgtg
     1741 caggggagcg tgtttcccga ccagcgccgt cgctcgctcg aggagctcat ggccatcggg
     1801 ttcgacggct acgcggtggg gggcctggcg gtcggcgagc cgaaatcggc catgtgggaa
     1861 acgctcgagg cgctggagcc gcacctgccc aaggaccgtc cgcgctacct catgggtgtg
     1921 ggcacccccg aagacctgat cgagggggtg cggcgtggca tcgacatgtt cgattgcgtg
     1981 cttcccaccc gcaacgcgcg caagggcacc gttttcacgc gggacgggcg actggtggtc
     2041 aagaactcgt cgttcgcccg ggacgagcgc ccgctcgatc ctgactgcaa ttgcctcacc
     2101 tgcacccgct acagccgggc ctacctgcga cacctctttg ccaccgggga gctcctgggg
     2161 cctcgtctgg ccagcctcca cagcgtatac ttctacctgg ccctgatgac cgaactgcgc
     2221 accgcgctcc gcgagggtcg attcgattcc tggagccgcc ggtttctggc ccgctaccgc
     2281 ggcaccgact gatcaccccg gcgtcacccg ctcagtaaaa actccataca cccgattcgg
     2341 aggtccgaac gcgatgttgc ttccggccac gttgctggct ttcatgcccc tcacgctccc
     2401 gctcatggct cccgcgcccg tcggcggcga ggcgcccaat cccctcatcc agatgctccc
     2461 gatcgtcctc atcttcgtca tcttctattt tctgctcatc cggccgcagc aggcccagca
     2521 gaagaagcac caggagatgc tcaagaacgt gaaaaagggg gatcgcatca tcacgtccgg
     2581 cggcatctac ggtcgcgttg tcggcgtggg cgacaacgac gtcacggtgc agatcgccaa
     2641 ggacgtggaa gtccagctcc agaaggggtc catcaccgcg gtggtcgctg ccgagggcgg
     2701 caagtagctc cgatggcgcg cgaggaactc catctccggc tctacggcga tcctgtcttg
     2761 agacagaagg ccgcggatat caccgtcttc gacaagtcac tggcgaagcg ggccgaatcg
     2821 atgttcgaga tcatgtacga gaatgacggc atcggcctgg ccggcaacca ggtgggcctt
     2881 ctcgagcgcc tcatcgtgct cgatgtgagc ctggacgacg agaaccgcgt ccggatggcg
     2941 ctggccaacc cgcgcatcct gacacgggcg ggggaggaca cggccgagga agggtgcctc
     3001 tcgattcccg acgtgcgcgc cgacgtcacc cggccggacg tcctgaccgt ggaagccgaa
     3061 gggctcgacg ggaagacgat ccgcttcgag gcggaagggc tgctggcccg ggccatccag
     3121 catgaaatcg atcacctgga tggcgtcctc ttcatcgacc ggatcagcgc cacgcgccgc
     3181 aagctcctcg aagggcggct gaaggagatc gcccgggagt ccgcgagctg acgggcccgc
     3241 ccgtcagctc gaggaaggct tcgatgcgcg ttgtcttcat gggcacgccc gttttttcgg
     3301 tcccgtgcct tgtggcgatg gtcgaggccg ggcataccgt tccgctggtc gtcacgcagc
     3361 cggaccgccc gaagggtcgg ggccgggaac tttcgcccac gccggtcaag caggccgcgc
     3421 tcgaccggaa catcgcggtg ttccagcccg aacggccgaa tcgcgatgac gcggtggcca
     3481 cgatcgaagc cgcccggccg gatgtgctgg tcgtcgtggc ctacggcgcc atcctgaaag
     3541 cccgcctcct cggcctcgcg ccgaaagggg cggtgaacgt gcacgcaagc ctgttgcccc
     3601 gtcaccgcgg gatgagcccg atccaccggg ccatcctcga tggcgatgag gaaacgggca
     3661 tcaccacgat gttcatggat gaaggagtgg atacgggcga catgatcctg atcgaaacga
     3721 cacccatcgc cccgaacgaa accacgggcc aattgcacga ccgactggcc cagatgggag
     3781 cccgcctgct ggtcgatacg ctggcgcgga tcgaggccgg cacggccccg cgcacgccgc
     3841 agcctcccct cggcgccagt tacgccggtc gcatcgacaa gatcgacggc gtgatcgact
     3901 ggagtcgcaa cgccgcccag gtgcacaacc gcattcgggc gatgactccg gcgcccggcg
     3961 ccacggggag cctcgaaggg cagccgctcc tcgtggcggg aagcctcctc gtggacgaat
     4021 ccgtgcagga tgccatccct cacggcgata ccacccgcac gcccggcggc gaacccgcca
     4081 ttcccggcac cgtgctggcc gtcatgcgcg atgcgctccg cgtggccacc ggtcagggag
     4141 ccatcgacat ccacgaagtg cgccccgcgg gcggcagaac tatgtccgcc tccgcctacg
     4201 cgcgtggccg cggcatcgcc cccggcagcc gtttctacgc accgcctgca tcgcctgggg
     4261 cgccgggagc cgcacgatga accgtcccta tcgtccgcgc cgcgaggcgc cccccaagac
     4321 accgcgcgaa gtggcgctcg aagcgctcta cgcggtggac cgccgccatg cgttcagcga
     4381 tcgcctgctc cacaagctcc tgcgcgatca cccgatggac ccgcgcgacg ccgcactggt
     4441 caccaacatc gtgcgcggca cgctccgctg gcaggggcgc atcgacggcc tgctgaacca
     4501 ctacatcaag atcggcctcg acgcgctgcc gcccatgatc cagaacatcc tgcgcatggg
     4561 cgtcttccag atgctctaca tggaccgcat cccgcccagc gcggcggtgg acgagagcgt
     4621 gaaactcgcg aagaagttcg gtcatcccgg caccgccggg ctcgtcaatg ccgtgctccg
     4681 caagatggtc accgaaaagg accgtcttcc cgagccgcgc ctgggagaca accccgcgcc
     4741 cgcgcttgcc acgatctact cgcacccgca gtggatgatc gaccgctggc tcctccgttt
     4801 cggcctggat gaaacgaagg agctgctcga ggccaacaat tcaccgccgc cggtcacgat
     4861 ccgcatcaac cccaacaaga ccacgcgcga ggaattcctg aagcgcctcc acgacctctc
     4921 gctcaccgcg gaagtcggcg agcaccacaa gagcaccatc aaggtgcacg gcgattttgc
     4981 gcctgcgtcc gtgtcgagtt tccaccaggg gctcttcacc gtgcaggacg agagcgagag
     5041 catggtggtg gacctgctcg acccgaggcc gggggagcgg atcgtggacc tctgcgctgc
     5101 ccccggcggc aagaccagcc agattgccga gctgatcggc cccggcggca cggtcatcgc
     5161 cgtggagggg cagaaggggc gcctcaagcc attgtccgaa aacaagttac ggatgagcat
     5221 ggataatgcc cagctggtcc tggccgatgg ccgcaggctg gcgctccgga agccggtgga
     5281 caaggtgctg atcgatgccc cctgctccgg actcggcgtc ctggccaagc gagccgatgc
     5341 ccgctggcgg aagaccgaag ccggcatccg gcagatcgcg gaactgcagg cccagctcct
     5401 cgaggccggc gccgcgctgg tgaagcccgg cggcgtgctc gtctatagtg tctgttccat
     5461 ggaacccgaa gagggagacc agcaggtccg cgccttccag aagcgccacc ccgaatggcg
     5521 catcgacgac gccgcccagt acgtcccggc ggatgtcgtc accgacagga tgctccaggt
     5581 cttcccgcac cgccaccaca tggacggcgc cttcgccgcc cgcctggtga aagaggggta
     5641 atgaacaccc cgccggaacg catcgacgcg cccggcccca ggagccgccc gcagctggcc
     5701 ggcctcatcc agattgccct cggcctggtt gccgccctcg tggtgacggt catcctcttc
     5761 aacaacgtgg tcatgcccgg cttcgtcggc cgcggcaacg tgacccgggt gcccgacgtc
     5821 accggcaagg agctgaccca ggcccagtcg ctgctcacga aggcgggcct tacccccggc
     5881 gaaacccgcc aacgcaacga ccccaagccc gccggcaccg tcatccgcca gcaaccgcag
     5941 gccggcaact ccgtgaagga cgggcgcccc atcatcctga ccgtgagcct cggcgagccc
     6001 ggccgctccg tcccaacgct ggcgcaggaa tcgatccgca acgccgaact cgccctgggc
     6061 gacgtggacc tcgtgctcgg ccaggtcacg cgtgccccga ccgacaacat tccgaccgac
     6121 ctcgtcgtgg ccagccagcc gcccgccggc accacgctcc cccgcggcag caccgtcgac
     6181 atcctggtat ccgccggcac cagccggcgg tccgccggat atgtcatgcc gaacctcgtg
     6241 ggccgggaaa cacgccctac gcgcgatcgg ctgttggacg ccgggttcaa ggtgacggtg
     6301 gatgaaaaat cgttcggatt ttttcgcgtc ggaaacacgc acgtgacgga gcaggatccc
     6361 gagcctggct cccgcgtgat gccctgggac accatctggc tgaaggggga gtgaccgcgt
     6421 gattcccgac cgccccctga tcgcccccag catcctctcc gccgacttca cgcagctggc
     6481 cgagcagatc cacctgatgg agactgccgg cgcccagttg ctgcacctgg atgtcatgga
     6541 tgggcacttc gtgcccaaca tcacattcgg cccgatggtc gtggaggcgg tgaaccggat
     6601 cacgccgttg ttcctggatg tgcacctcat gatcgccgag ccgcagaagt acgccgaagc
     6661 gttcgtgaag gcgggggcgg atcggttgct ggtgcagatc gagacgcagg cggatcccgt
     6721 gccgctgctg gatcggattc gtgggctggg cgcagaggcc ggactggtgc tcaacccgga
     6781 gacgccgttt gaggcggttg agccgcacct ggcgcacatc gactgcctgc tcgtgatgag
     6841 cgtgcacccc gggttcggcg ggcaggcctt catggccgaa gtgctgccga aggtgcagaa
     6901 ggcgcgggcg ctgcgggaga tgcatgggtg ggattggccg atcgagattg atggggggat
     6961 tgattcggcg acggtcgtgc ccgccgtgga agccggcacc agcatcctgg ttgccggcaa
     7021 tgcgatctac aagtatccgg acccgtccga cgccttcacc cgccttcggc tgacggccga
     7081 gctggccggc gagtcgagcg gccggccaag gggtctgatt cggacggatg aaggcttcct
     7141 ctagagactg gcgtccccgc tcttaacctg acttctggag gagactcaaa tgaccttccc
     7201 cggtttcgac gcccgcccgc tcgcgcttgc ccttgctgtc ggcatcgcgt tcgtgggcct
     7261 cccgctgctc ggcgtcgttg gccccagcga cgtgcgctcc gccgaatatg tatacgagac
     7321 cgccgactcg ctccacccgc acatccgcta cgacgacggc tcggtgagcg tgaacgatcg
     7381 ctgcgccgtc cgcatgacga agctctcgcg gtcgatgcgc cccatctacg tgaacgggaa
     7441 accgatcggc ttctgctgag gcccctgccc gaaggctttc ggtgaaagcc ccgagatgta
     7501 cctcaaggcc cgcggcatcg acgttcgtga ctatttcgat ccggacatcg tggcggtgct
     7561 cgacatggag catcgatcag cggtgaatta cgagctctac ttcttcggca acagccagtc
     7621 gaaggcccgc ttcgacaagg acatcctgaa gcattgcggc gtggtgaccg acccgatcat
     7681 ccgtacgcgc ttccggccaa gccacctgag cccgcacgag gactacatgg ggcggcgcta
     7741 ttacttcctg acccacggca cgcaggccga attcgccgcg cacccggaca gctttgcggt
     7801 gcggcagggg atgtagggca caaatcgctt gattcgggct tgtccgaccg tcattctggt
     7861 cgcgtgacac gcatccggat cggcaccctc gcgctggcaa ccgtgctggc caccgcattt
     7921 tcagccgtgg ccctcgtcat gggcgattcg gtcacgccgc tctcgccggc cgatgtcacc
     7981 ccccccacgg taacggtcat gtcaccgaat ggcggcgaat catggacggc cgccaccacg
     8041 cactccgtga catggaccgc caccgacgat gtgaacgttt cgaccgtcga catcctctac
     8101 gaagatgcgc cgggcgccgg ctggacgctg atcgcccgcg acctcgagaa caccggaagc
     8161 tggatctggc cggttcacaa cacgcccggc accggcgtgc gggtgcaggt acgcgcccgc
     8221 gatacctccg ccaatgtcgg ggccgattcg agcaacgcgg tgttcacgat cctcatcgag
     8281 ccgggggggc ggcttcccac cacgctgcgc gatttccgga tgccgggtac gcagcccctc
     8341 gccatcaaca acttcaaccg cagcagctcg tgcaatggct gccacatcgg gttcaatgcc
     8401 gccaccgagc ccggcttcgg cttcaaggga agcatgatgg cgcaggcggg gcgcgatccc
     8461 ctcttctacg cgtgcatggc catcgccaac caggatgccc gctcgtcggg cgacctctgc
     8521 atccgctgcc actcgccgtt cggctggatg gaggggcgga gcaatcccac cgatggttcc
     8581 gcgctcaccg cagcagaccg cgacggcgtg tcgtgcgatc actgccatcg catggtcgat
     8641 ccggtctacc agctgggaat cagcccggtg gaagacgcgg ccgtgctggc ggccatgccg
     8701 gtggctgatc ggccgacggg ggcccacggc gggcagtacg tggatgatcc gaacgtgagg
     8761 aagcgcgggc cgtacgccga cgccaacccg ccgcatgcga agctggcctc gaacttccac
     8821 cgttcgtcgg acctctgcgc cacctgtcac gacctgtcga acccggtgta cacccgcacg
     8881 ggtgacgacg actatgcacc cggttcgttc aacagcgcgc ccggcatggt ggcgtcggac
     8941 acgcatatgc cgatcgagag gacgttcagc gaatgggcgg ccagcaactt tccgagcggt
     9001 gtgtacgcgc ccgagtttgc cggcgtgaag ccgggcggca tcgtgagtac ctgccaggat
     9061 tgccacatgg cggatgtcgt ggggaaagga tgctccagcg gatccacccc ggtgcgttcg
     9121 gacctgggtt tccacgactt caccggcggc aattcatgga tgccaggggt catcggtgcg
     9181 ctctatccct cggaaaccga ccccgccgcc ctcgcggcgg ccgccacgcg cgcccgcggc
     9241 atgctggcca agtcggccgt ggtggacgtg ggcctgagcg cagccaccgg caacctggtg
     9301 gccaccgtca cggtcacgaa tcgcacgggg cacaagctgc cgaccggcta cccggaaggg
     9361 cgccgcatgt ggctcaacgt ggtggcgtac gacggcgtca acacgaaagt ctacgaatcg
     9421 ggggcgtaca acgcgtcgac cggtgtcctg accgaagacg cggccgcgcg cgtgtacgaa
     9481 gtgcaggcgg ggctttccgc accgatcgcc acccaggcga gcctcaccgc cggcccgtcg
     9541 ttccacttcg tgctgaacga caccatctac aaggacaacc gaattccgcc ggcgggcttc
     9601 accaatgctg aattcattct cttcggaggt cgccccgtcg agccgggtca tcccgcgccg
     9661 cgctatgccg acggacagaa ctgggatacc gcaagctaca cattgccgag cacgacacgc
     9721 aaagtcgtgg ccacgctccg ttaccagacg acgtcaaggg aatatgtgga atttctacgg
     9781 gacgcgaacg tgaccaacac cgcggggcag gtgatgtacg acgcatgggt ggcgcatggg
     9841 aaatcgacgc cggtgacgat ggccgccgat tcgatggtga tcggggcgac gggcgttccg
     9901 gatgaggtcg cgctcacggc gccgacgaca atcggaccgt tgtcggccat gtcgaacccg
     9961 ttccgcgagt tgctggcgct gtcgttcgaa ctgagtgggc ccgcacgcgt gtcgtggcag
    10021 gtattcgacg tgcgcggtcg ccgcatcgcg gcgggagaac cggggatgct gggcggtggc
    10081 gcgcaccgcc tcgactggga tggccggatg gaagaaggct tggcgccggc ggggatctac
    10141 tgggtgcgcg tgacggcagg agcggaggag caggtcctgc gcgtcgtgcg ggtgcggtga
    10201 cggcgccgct ggtacgccct acaaccgccg agtccccgcc tccgtcacat gccggaacac
    10261 cgcctcgagc gtgtcatccg gcgcctgcag ccgccaaacg gccgctccag atgccgtgac
    10321 gagggccggc aactccggat aaagagtatc cggccccgac gtcaggaacg acagcgaact
    10381 cggcccggtg atgcgcacct cgagcgtgtc cggtcgatcg agcaacagtc gccccagcgg
    10441 ccgcggatcg ccgacgacat ccaggtgaac acgatgggga tgtcgatcgc gaagcgatct
    10501 caacaggtct tccagctgcc cgaacccgag cagacgcccg tgtgccagca ccgccacgcg
    10561 actcgtgagc gtttcgattt ccgacaggac atgcgatgtc agcatcacgg tgcgcccctc
    10621 cgcggcgagc gcgcggatga gctcggtgag ctgtcgtcgg ctcggcggat cgacaccgag
    10681 gaacggctcg tccagcagca ccagtgcggc atcgcccgaa agcgcccacc cgagcttcag
    10741 tcgctggcgc atgccccggc tccaggtgcc gtagtcgcgt ttgccgtcgg caaccaggcc
    10801 ggcaacttcg agggccttgt cggcgcgcg
//
LOCUS       DASVOD010000164         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_740846, whole genome shotgun sequence.
ACCESSION   DASVOD010000164 DASVOD010000000
VERSION     DASVOD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740846"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2039)
                     /locus_tag="VF720_03385"
     CDS             complement(<1..>2039)
                     /locus_tag="VF720_03385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX7878423.1"
                     /translation="TIFAGIGAVLTFFNGLNSIPLAMYDYETTQAWGAFLGNTIVGAV
                     MGGLGVAIWIALFGAAGEPLYRRDLPHVPSLNRYLSRRTIGTKSFFRALVLGYTLVSA
                     FMAYQCVFYVVAARFGAWAPAETPYSNLLNTQFPWITVLLIGFLPAVTEEFSNRMFSI
                     PLYTRMMHFPWLRPLGVILAGFVWGFLHASYPNQPFWIRGVEVGLAGCVIGVLMLRYG
                     VVPLLIWHFSVDAIYTALLLLRSGKPYFVVSGALAAGLLLVPLLAAVGLYLRRRGFTP
                     EDDLTNAAEGFHAGESTRREEDVAGDAAAEEVMAAPSDAPSDVPAAEMPPPMAPAPLP
                     DRRVSIRLLVLTALALALATIVLTWPTDYPPRRLDWPMGRNEALRVAADFLRANGADP
                     DTFRSGTMPVSGFDRYRDDGVNTPPDISDAIAHRYVTETGGLSAWERLVTTRLPASNW
                     ESRFVKSLSRHEWTVTVDPRRGRVTGFKRTFPDEEPGAELDEAAARLAARELIRSMEA
                     NEPDGHAGTWSEIAAKSDKRPHRRDWTFVFDDSSAAVDAGVPRLTTILAGDRPAGYGV
                     TLYIPEGYTRQRTLSTPLSTLLLVVRLLVMGGLFGLMAVEVIRSTRSASVPWRALLGL
                     ALAATLPSALAQLNRLSVLVFTYRSEIPWNLFLLTGSIGLVLGVIMQLMAVIAALL"
BASE COUNT          372 a          635 c          744 g          288 t
ORIGIN      
        1 agcagcgcgg cgatgaccgc catcaactgc atgatcaccc cgaggacaag cccgatggat
       61 ccggtcagga ggaagaggtt ccaggggatc tcggagcggt acgtgaacac cagcaccgac
      121 aggcgattga gctgggccag agccgacggg agggtggcgg cgagggccag accgagcagt
      181 gcccgccagg gtaccgatgc cgaacgggtg ctgcggatga cctccacggc catcagtccg
      241 aacaatccac ccattaccag cagccggacg acgaggagaa gcgtggagag gggcgtggag
      301 agcgtccgtt gccgcgtgta cccctcgggg atgtagaggg tgacgccgta gccggccggt
      361 cggtccccgg cgaggatggt ggtcagtcgc ggcacgccgg cgtcgacggc ggcgctgctg
      421 tcatcgaaga cgaaggtcca gtcgcggcga tgggggcgct tgtcgctttt cgcggcgatc
      481 tcgctccagg tgccggcatg cccgtccggc tcgttggcct ccatcgagcg gatgagttcg
      541 cgggccgcaa ggcgcgcggc ggcttcatcg agttccgcgc cgggctcctc gtcggggaag
      601 gtccgcttga agcccgtcac tcttccacga cgcggatcga cggtgacggt ccattcgtgc
      661 cggctcaacg acttcacgaa gcgactttcc cagttggatg ccggcaggcg ggtggtgacg
      721 agtcgttccc acgcggacaa tccgcccgtt tcggtgacgt agcgatgggc gatggcgtcc
      781 gaaatgtcgg gaggcgtgtt cacgccgtcg tcccggtagc gatcgaatcc cgagaccggc
      841 atcgtgccgc tccggaaggt gtcggggtcg gcgccgttgg cccggaggaa gtcggcggcc
      901 acccgcaacg cctcgttccg tcccatcggc cagtcgaggc gacggggggg atagtcggtg
      961 ggccaggtga ggacgatcgt ggccagggcc agcgcaaggg cggtgagaac gagaagccga
     1021 atcgatacgc ggcggtccgg caggggggcc ggcgccatcg ggggcggcat ctcggcagcc
     1081 ggcacgtcgg atggcgcgtc ggatggagcg gccatgacct cctccgcggc cgcatcccct
     1141 gcgacatcct cctcccgccg ggtcgattcc ccggcgtgga acccttcggc cgcgttggtg
     1201 aggtcgtctt cgggcgtgaa ccctctccga cgcaggtaga gtcccacggc cgccaacagc
     1261 ggcacgagaa gaagccccgc cgccagcgcg ccactgacca cgaaataggg cttcccgctc
     1321 cggaggagca ggagcgcggt gtagatggca tccaccgaga agtgccagat gaggaggggc
     1381 acgacgccat agcgcagcat cagcacgccg atcacgcagc cggccagtcc cacttccacg
     1441 ccgcggatcc agaagggctg gttgggatag gaggcgtgga ggaatcccca tacgaagccg
     1501 gccaggatga cgcccagcgg ccggagccac gggaagtgca tcatgcgcgt gtagagcggg
     1561 atcgagaaca tccggttgga gaattcctcg gtcaccgccg gcaggaatcc gatcaggagg
     1621 accgtgatcc acggaaactg cgtgttgagc aggttcgagt agggggtctc ggccggcgcc
     1681 caggcaccga aacgggcggc caccacgtaa aagacgcact gatacgccat gaaggcgctg
     1741 accagcgtgt agccgagcac cagcgcccgg aagaaactct tcgtgccgat cgtccggcgg
     1801 gagaggtagc ggttgagcga ggggacgtgc ggcaggtcgc gtcggtagag cggctcgccg
     1861 gccgcgccga acagggctat ccagatggcc acgccgaggc cccccatgac cgccccgacg
     1921 atcgtgttgc cgaggaaggc gccccaggcc tgcgtcgttt cgtagtcgta catcgccaga
     1981 gggatcgaat tgaggccgtt gaagaaggtc aggacggcgc cgatcccggc gaagatcgt
//
LOCUS       DASVOD010000165         7377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_897481, whole genome shotgun sequence.
ACCESSION   DASVOD010000165 DASVOD010000000
VERSION     DASVOD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7377
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897481"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="VF720_03390"
     CDS             <1..952
                     /locus_tag="VF720_03390"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7878424.1"
                     /translation="NVLGEEAFLADVDKTVEDWLPMMDALLTEDDVDLYVHVWDFTDR
                     IGHMFWRTIDPTSPAADSLADAEWNDVMIRTYQRMDSIVGRVREKLPEGSTLLVCSDH
                     GFNTWHKSVNYNTWLVHNGFMTLKGPEGGRALTLDDLFGQGEFWPNVDWSKTKAYALG
                     LGDLYVNLKGRESQGCVDPADYETVRNELIAGLEAFVDSANGERPVRRVIRREDVYSQ
                     FDPKLIPDLFLANSEKYRVSWQTSLGGIPGQLFEINDKKWSGDHCSLDAEITKGIFLS
                     SARYQAPAPNILDLYPTILNELGVTVPAGLDGTPLPRRTP"
     gene            972..1796
                     /locus_tag="VF720_03395"
     CDS             972..1796
                     /locus_tag="VF720_03395"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7878425.1"
                     /translation="MSFRSPHDSAGNGFADRFTPAIRMLVIACMLVFLLQALFHGPGP
                     VLERLFGLVPADLFGRWHLWQPLTYVFLHAGLFHLLFNLLALVMFGGEIEQRWGTGAF
                     LRYYFVCGAGAALTHWMVGMHSTVPVIGASGAIFGVLLAFGMLFPDRQILLWFVIPIP
                     ARYLVVLFGFIELVGAAAGPADGVARFAHLGGLITGWIYLKSESWLWPIRRRFGAIKR
                     KQAEKASLAAIEDRESKQAVIDAILEKIQSEGMGALTDKEKKILHDAAQRGRRPRG"
     gene            complement(1797..3101)
                     /gene="hemL"
                     /locus_tag="VF720_03400"
     CDS             complement(1797..3101)
                     /gene="hemL"
                     /locus_tag="VF720_03400"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX7878426.1"
                     /translation="MTAPRAGQRSRALFGRALDLFPGGVDSPVRAFRSVGGDPFIVAA
                     AEGARLTDVDGNAYVDWVMSWGPLILGHADPAVVEALEARIRRGTSYGAPCEEEVAMA
                     EAVVARFPSIEKLRFTSSGTEAAMSAVRLARAATGRDRLIKCDGCYHGHVDALLVKAG
                     SGLATQGLADSAGVPANVAAATTVVPFNDLEAVAAALRGAPGEYAAVLVEPVAANMGL
                     VPPADGYLEGLARLCSDAGTLLVFDEVITGFRVAPGGAQERYGVTPDITILGKIIGGG
                     LPVGAYGARRELMALVAPDGPVYQAGTLSGNPIAMTAGLVTLARLAPPGPYTALEATA
                     RALADDLRRMAREAGLSVIVHQLASLLTVFFAEGPIRNYTDAAGADRARFATFHRAML
                     ERGVFLPPSQFEAWFLSTAHGQSDLEATRRAAAESFRVVAAG"
     gene            complement(3098..3547)
                     /locus_tag="VF720_03405"
     CDS             complement(3098..3547)
                     /locus_tag="VF720_03405"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX7878427.1"
                     /translation="MFPNDANPAGTVMGGRVMHLIDVAAAVAAGRHARTLCVTASADH
                     IDFRSPVRVGDLLILKASVNYVGTTSLEVGVRVEVEDRKTGQRRHTSSAYLTFVALDD
                     ARQPMVVPRLGLETEVDRRRHTEAERRRLARLIARGKEFPPGHSPTP"
     gene            complement(3615..4592)
                     /locus_tag="VF720_03410"
     CDS             complement(3615..4592)
                     /locus_tag="VF720_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX7878428.1"
                     /translation="MSHILVVAEQRGGSLRKAAQEALSHARRLADASAGRVTAVIAGS
                     GIAGLSTEAARFGADHVLLADHADLANYSTEGYALAAQRAFEKTSPTLVLMGASSMGK
                     DLAPRLAARLRLAYLADVVEMSGTGADVTAVRPMYAGKIRATVAPAGGVVPLVTIRPN
                     VFRPAPADGRTATTEAIDMSGLAIRARVVETKSEAGGQIELTEADVVVSGGRGIKGPE
                     NFPLIRELADAFGGALGASRAVVDAGWIDHAHQVGQTGKTVSPGLYVACGISGAIQHL
                     AGMSSSRIIVAINKDAEAPIFKVADIGVVGDVFDIVPKLTAEVKRIRSH"
     gene            complement(4624..5394)
                     /locus_tag="VF720_03415"
     CDS             complement(4624..5394)
                     /locus_tag="VF720_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX7878429.1"
                     /translation="MKIVVCVKQVPATDSRIKPMADGSGIDPAGLEYVVNPYDEFAIE
                     EALRIKEKLGGEVVAIGIGGARAEEGLRTCLALGCDRARVLKDESLSGADALTIAGAL
                     AAIIKSEAPDLVLTGKQAVDDDQMAVPAMLAELLDWPQATVVIKLEVAADGKSATAER
                     EIEGAVEVIQLPLPAVIAAQKGLNEPRYASLKGIMAAKKKAVEPVDASAVTAGAPAVR
                     TVSVTPPPPRPEGRRLDGDAESQVKELVRLLHEEYKVI"
     gene            complement(5470..6417)
                     /locus_tag="VF720_03420"
     CDS             complement(5470..6417)
                     /locus_tag="VF720_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX7878430.1"
                     /translation="MKKQWINELSAPGTVNGQFAVASKTLRDFARGRVLSMTLADRTG
                     SIAAVLWDDAESIERSLKPGDIVKVEGSVQEYRDALQVKIDRIGFVDRGTVDLGDFRE
                     SLADAEQVEARLRGQLATITNPWLGKLVNSFLDDADFMARFRVAAAAKKWHHGFFGGL
                     LLHTTELVELAAAVTPLFPKADRDLVVTGAFLHDIGKIHELEGDLAVDYSTAGRLIGH
                     IVMGSQMALDRVRRIEGFPAVLSMHLQHLVLSHQGELSFASPVVPKSLEAILLHHMDD
                     LNAQANAFMRIIGDTKKREATWSDYQNIIDRQIWTGDVP"
     gene            complement(6414..>7377)
                     /locus_tag="VF720_03425"
     CDS             complement(6414..>7377)
                     /locus_tag="VF720_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878431.1"
                     /translation="FPVVHGLLIAVGAFTLSVSVAAASVLAIDLRFASAQWVQQLWRP
                     VLLGGILVALAAGSLTLDVVLFLYAGAGLLTLLLVRRTLAKVPRGADPLPFGRLVREG
                     AVFFGLFLTSSLMLRLDAFFLFGLISEEALGRYAAASNIALTGYGILAAGVAQVVTPR
                     IASGEGLALRRLLLTLTLLAVGAGALIVLFGTPVIHLVGGQEKYPGDFSGLLGILCIA
                     GIIQVAYVVPSAWLGATAPLPALKLFLLVNVVSLVINAILNSLLIPRWQLEGAALSTA
                     LSWAWRLGWALTFVAWIRRVRARRGTSTPATPTGADIIARGELT"
BASE COUNT         1195 a         2488 c         2530 g         1164 t
ORIGIN      
        1 aaacgtgctg ggcgaggagg ccttcctggc cgacgtcgac aagacggtcg aggactggct
       61 tccgatgatg gacgcgctcc tgacggaaga tgacgttgac ctctacgtgc acgtctggga
      121 tttcaccgac cggatcggcc acatgttctg gcgcaccatc gatcccacgt caccggcggc
      181 cgactcgctg gccgacgcgg agtggaacga cgtgatgatc aggacctacc agaggatgga
      241 ctcgatcgtg gggcgtgtcc gggagaagct gccggagggc tccaccctgc tcgtctgctc
      301 cgaccacggc ttcaacacgt ggcacaagtc ggtgaactac aacacgtggc tcgtgcacaa
      361 cggcttcatg acgctgaagg gtccggaagg ggggcgcgcc ctcacgctcg acgacctgtt
      421 cggccagggg gagttctggc ccaacgtgga ctggtcgaag acgaaggcgt acgcgctcgg
      481 cctgggggac ctgtacgtca acctgaaggg gcgcgagtcg caggggtgcg tcgatccggc
      541 cgactacgag acggtccgca acgagctcat cgcggggctc gaggccttcg tcgattcggc
      601 caatggcgag cggcccgtcc ggcgggtcat caggcgggag gatgtctatt cgcagttcga
      661 tccgaagctc atccccgacc ttttcctggc caacagcgag aagtaccgcg tgtcgtggca
      721 gacctcgctc ggcggtatcc cggggcagct cttcgagatc aacgacaaga agtggagcgg
      781 cgaccactgc tccctcgacg ccgagatcac caagggaatc ttcctctcga gcgcccgtta
      841 ccaggcgccc gcgccgaaca tcctggacct ctacccgacg atcctgaacg agctgggcgt
      901 gacggtcccg gccggcctcg acggaacgcc ccttccgcgg cgcacgccgt gacgaccggg
      961 agtcgctgac catgtcgttc cgttcgcccc acgactcggc ggggaacggc ttcgcggacc
     1021 gcttcacgcc cgccatccgg atgctggtca tcgcctgcat gctggtcttc ctgctgcagg
     1081 ccctgttcca tggtccaggc cccgtgctcg agcggctctt cggcctcgta ccggcggacc
     1141 tcttcggaag atggcacctc tggcagccgc tcacctacgt cttcctgcac gccgggctct
     1201 tccacctgct gttcaacctg ctcgcgctgg tcatgttcgg cggcgagatc gagcagcgct
     1261 gggggacggg tgcgttcctg cgctactact tcgtgtgcgg ggccggggcc gccctcaccc
     1321 actggatggt cggcatgcac tcgacggtgc cggtgatcgg cgcctcgggg gccatcttcg
     1381 gcgtgctact ggccttcggc atgctctttc cggaccggca gatcctgttg tggttcgtca
     1441 ttccgatccc ggcccgctac ctggtcgtgc tgttcggctt catcgagctg gtcggtgccg
     1501 cggcggggcc cgcggacggc gtggcccgtt tcgcccacct gggagggctg atcaccggct
     1561 ggatctatct caagagcgaa agctggctct ggccgatccg gcgccggttc ggggcgatca
     1621 agcggaaaca ggccgagaag gcctcgctcg ccgcgatcga ggatcgcgag tcgaagcagg
     1681 cggtgatcga cgccatcctg gagaagatcc agtccgaagg gatgggagcg ttgaccgaca
     1741 aggaaaagaa gatcctgcac gacgccgccc agcgcgggcg tcgtcccagg gggtgatcag
     1801 ccggccgcca ccacacggaa cgattcggcg gccgcgcggc gggttgcctc gagatcggac
     1861 tggccgtggg ccgtcgacag gaaccaggct tcgaactggc tggggggcag gaacacgccg
     1921 cgctcgagca tcgcccgatg gaaggtggcg aagcgtgcgc ggtcggcacc ggccgcgtcc
     1981 gtgtagttcc tgatcggccc ttccgcgaag aagacggtga gcagcgaggc aagctggtgc
     2041 acgatcacgc tgagtccggc ttccctggcc attcggcgca gatcgtcggc cagtgccctg
     2101 gccgttgctt cgagggccgt gtaaggcccc ggcggcgcca ggcgtgcgag cgtcacgagg
     2161 ccggccgtca tcgcgatcgg gttccccgac agcgttcccg cctggtagac ggggccgtcg
     2221 ggcgccacga gcgccatcaa ttcccgccgc gcgccgtagg ccccgaccgg aagtcccccg
     2281 ccgatgatct tgccgaggat ggtgatgtcg ggcgtcacgc cgtagcgctc ctgggccccg
     2341 cccggcgcca cgcggaaccc ggtgatgacc tcgtcgaaga cgagaagcgt cccagcgtcg
     2401 gagcagagcc gcgccagccc ttcgagatat ccatccgccg gcggcacgag gcccatgttg
     2461 gcggccaccg gctcgaccag cacggcggcg tattcgcccg gcgccccacg aagcgccgcc
     2521 gcgacagcct cgaggtcgtt gaacggcacg acggtggtgg ccgcggccac gttggccggc
     2581 acgcccgcgc tgtcggcgag cccttgcgtg gccaggcccg atcccgcctt cacgagcagg
     2641 gcgtccacgt ggccgtggta gcagccgtcg cacttgatga gccggtcgcg accggtggcg
     2701 gcccgggcca ggcggacggc gctcatggcc gcctcggtgc cggaggaggt gaagcggagt
     2761 ttctcgatcg aggggaatcg ggccacgacg gcctcggcca tcgccacttc ttcctcgcag
     2821 ggggcaccat acgaggtgcc gcggcgtatc cgtgcctcca atgcctcgac gacggccgga
     2881 tcggcatgcc cgaggatcag cgggccccag gacatcaccc agtcgacgta ggcgttgccg
     2941 tccacatcgg tgagccgggc gccctcggcg gcggccacga tgaagggatc gcctccgacc
     3001 gaacggaagg cccggaccgg gctgtcgacg ccaccgggaa agagatccag cgcgcgtccg
     3061 aacagggccc tcgagcgctg acctgcgcgt ggagcagtca tggcgtcggg gagtggccgg
     3121 gtggaaattc cttgccccgt gcgatcagtc gcgccaggcg ccggcgctcc gcctctgtat
     3181 gccggcggcg gtcgacctcg gtttccagcc cgagtcgcgg cacgaccatc ggctgacgcg
     3241 cgtcgtcgag cgccacgaag gtgaggtagg ccgacgacgt gtggcgacgc tggccggtct
     3301 tccgatcctc cacttccacg cgcacgccca cttcgagcga ggtggtgccc acgtagttca
     3361 cgctggcctt gaggatcagg aggtcaccca cgcgaaccgg cgaccggaag tcgatgtggt
     3421 cggccgaggc cgtgacgcag agcgtacggg catgcctgcc cgctgccacg gcggccgcca
     3481 cgtcgatcag gtgcatgacg cgccccccca tgacggtgcc ggccgggttg gcatcattcg
     3541 gaaacatgat ttccaccatc tcgacccggc tctccgagac cggcctttcg gggcgttccg
     3601 ggtgggacgt cacgtcagtg ggaccggatc cgcttcacct cggcggtgag cttcggcacg
     3661 atgtcgaaca catctcccac cacgccgatg tcggccacct tgaagatggg ggcttcggca
     3721 tccttgttga tggccacgat gatcctggac gacgacatgc cggccaggtg ctggatggcg
     3781 cccgagatgc cgcacgccac gtagagtccc ggcgacaccg tcttgccggt ctgcccgacc
     3841 tggtgcgcat ggtcgatcca tccggcatcc accacggccc gcgaggcgcc cagcgcgccg
     3901 ccgaacgcgt cggccagttc cctgatgagc gggaagttct ccggcccctt gataccgcgg
     3961 ccgcccgaca cgactacgtc ggcctcggtc agttcgatct ggccgcctgc ttccgacttc
     4021 gtttccacca cgcgggcgcg gatggcgagg cctgacatgt cgatcgcttc ggtcgtggcg
     4081 gtgcgtccat cggcgggcgc cggacggaag acgttgggac gaatcgtgac cagcggcacg
     4141 acgccgccgg ccggggcgac cgtggcgcgg atcttcccgg cgtacatcgg gcggacggcg
     4201 gtcacgtcgg cacccgtgcc cgacatctcg acgacgtcgg ccaggtaggc cagccggagc
     4261 cgggcggcga ggcgtggagc gaggtccttc cccatggacg aggcgcccat caacacgaga
     4321 gtcggcgacg tcttctcgaa ggcgcgctgg gcggcgaggg cataaccctc ggtggagtag
     4381 ttcgcgagat cggcatgatc cgccagcagc acgtgatcgg cgccgaagcg tgccgcctcg
     4441 gtggaaagac ccgcgatgcc ggagccggcg atgaccgcgg tgacgcgccc cgctgaggca
     4501 tcggccaggc gccgggcgtg gctcagcgct tcctgggctg ctttcctgag ggaaccgccg
     4561 cgctgttcgg cgacgacgag aatgtggctc atgtttatct ccccggggcg caccgcgccg
     4621 ccgtcagatg accttgtatt cctcgtgcag caggcggacg agctccttca cctggctctc
     4681 ggcgtcgccg tcgaggcggc gtccttcggg ccggggcggc ggcggcgtga cgctgaccgt
     4741 gcgcaccgcg ggcgctcccg cggtgacggc ggaggcgtcg accggttcga ccgccttctt
     4801 cttggcggcc atgatcccct tgaggctcgc gtaacgcggt tcgttcaacc ccttctgcgc
     4861 ggcgatgacg gcgggcagcg ggagctggat gacttccacg gcgccctcga tctcgcgctc
     4921 ggcggtggcg gacttcccgt cggcggccac ctcgagcttg atgaccacgg tggcctgcgg
     4981 ccagtcgagc agctcggcca gcatggccgg cacggccatc tggtcgtcat cgaccgcctg
     5041 cttgccggtg agcaccaggt cgggcgcctc ggacttgatg atggcggcca gggcaccggc
     5101 gatcgtgagg gcgtcggcac cggagagcga ctcgtctttc aggacgcggg cgcgatcgca
     5161 accgagggcc aggcaggtgc gaagcccctc ctcggccctg gcgccgccga tcccgatggc
     5221 cactacctca ccgcccagct tttccttgat gcggagcgct tcctcgatgg cgaattcgtc
     5281 gtacgggttg accacgtatt ccaggccggc cgggtcgatc cccgatccgt cggccatggg
     5341 cttgatgcgc gagtcggtgg caggcacctg cttcacgcag accacgattt tcactggaga
     5401 tcctcccctt cgcatgagcg gaaaaagccg gcgaaggtta ccagaggggc cggaacccgt
     5461 ccatccgcgt cagggcacgt cccccgtcca tatctggcgg tcgatgatgt tctggtaatc
     5521 gctccacgtg gcctcgcgct ttttcgtgtc gccgatgatc cgcatgaagg cgttggcctg
     5581 ggcattcagg tcgtccatgt ggtggagcag gatggcctcg agcgacttcg gcaccaccgg
     5641 cgaggcgaag gagagctcac cctggtgcga gaggaccagg tgctgaaggt gcatggagag
     5701 gacggcggga aatccctcga ttcggcgcac ccggtcgagc gccatctggc ttcccatgac
     5761 gatgtggccg atgagccgcc ccgccgtcga gtaatcgacg gccagatccc cttccagctc
     5821 gtggatcttg ccgatgtcgt ggaggaaggc gccggtcacg accaggtcgc gatcggcctt
     5881 cgggaagagc ggcgtgactg ccgcggccag ctcgaccagc tcggtggtat ggagcaggag
     5941 cccgccgaag aatccgtggt gccacttctt ggccgcggcg gccacgcgga agcgcgccat
     6001 gaaatccgcg tcgtcgagga atgagttgac gagctttccg agccacgggt tcgtgatggt
     6061 ggccagctgc cctcgaagcc gcgcctcgac ctgttcggca tcggcgagcg attcacggaa
     6121 gtcgccgaga tcgaccgttc ccctgtcgac gaagccgatc cggtcgatct tcacctggag
     6181 ggcatcgcgg tactcctgga cgctcccctc caccttcacg atgtctcccg gcttcagcga
     6241 gcgctcgatc gattcggcat cgtcccacag gacggccgcg atcgatccgg tgcgatccgc
     6301 cagcgtcatc gagaggacgc gtccacgcgc gaagtcgcgc agcgtcttgg aagccaccgc
     6361 aaactgaccg ttcaccgtac ccggcgccga cagctcgttg atccactgtt tcttcatgtg
     6421 agttcccccc gcgcgatgat atccgctccc gtaggcgtgg cgggagtgga ggtaccccgc
     6481 ctggcccgaa cgcgtcgtat ccaggccacg aacgtgagcg cccagccgag tcgccatgcc
     6541 cacgacaggg cggtggaaag cgccgctccc tcgagctgcc agcgcggaat cagcagcgaa
     6601 ttgaggatgg cgttgattac aagcgaaacg acattgacca ggaggaagag cttcagtgcc
     6661 ggaagaggcg ccgtcgcccc gagccaggcc gaggggacga cataggccac ctggatgatc
     6721 ccggcgatgc agaggatgcc gagcaggccg ctgaaatcac ccgggtactt ctcctggccg
     6781 cccaccagat ggatgaccgg cgtgccgaag agaacgatga gcgcgcccgc gcccaccgcg
     6841 agaagggtga gggtcaggag cagtcgcctg agggccagcc cttcgccgct ggcgatgcgt
     6901 ggcgtgacca cctgcgccac tccggccgcc aggatgccgt agccggtcag cgcgatgttg
     6961 ctcgccgcag cgtaacgtcc gagggcctct tcgctaatca acccgaaaag gaagaaggca
     7021 tcgaggcgca gcatgaggct cgacgtgagg aagagtccga agaatacggc accctcccgg
     7081 accagtcggc cgaacggcag cggatccgca ccgcgcggca ccttggccag cgtgcgccgg
     7141 accaggagga gcgtgagaag tcccgcgccg gcgtacagga acaggaccac gtccagcgtg
     7201 aggctccccg cggccagggc caccaggatg ccgcccagca ggaccggacg ccagagttgc
     7261 tgcacccatt gggcggatgc gaaccggaga tcgatcgcca gcaccgaggc agccgccacc
     7321 gacacggaaa gtgtgaaagc ccccacggcg atcaggaggc cgtgcaccac cgggaaa
//
LOCUS       DASVOD010000166         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_271630, whole genome shotgun sequence.
ACCESSION   DASVOD010000166 DASVOD010000000
VERSION     DASVOD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271630"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF720_03430"
     CDS             <1..398
                     /locus_tag="VF720_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEX7878432.1"
                     /translation="VCGPTTLMPVDVESMGVTVCHRVEEALEGAHVVNILRIQQERLE
                     SNLLPSLREYALQFSVTHDRLRVARPDILIMHPGPMNRGIEIAQDVAEDERNVILDQV
                     TNGVAVRMAVLYHLSGGESLLPVGIEGVA"
     gene            398..1756
                     /locus_tag="VF720_03435"
     CDS             398..1756
                     /locus_tag="VF720_03435"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX7878433.1"
                     /translation="MSSRSFQRLALWDSHDRPLFLSGADVRDPLTGREERQDLWIVNG
                     RITFRKPEGTTPVAFDLTGAVLFPRLIDVHVHLREPGFEEAETVATGALAAVKGGFSA
                     VCCMPNTNPPLDDAATIRFVLEQAARAGGATVYPFACITRGRKGEALADMFELAEAGA
                     RGFSDDGSPVMNAELMRRALEYSTMLDLPIVAHEEDSHLAAHGCVHESSVATRVGLKG
                     IPREAEDVMVARDIALVRLTGGRLHICHVSSKHTVDLVRQAKAEGLPVTAEVTPHHLV
                     LTHERVATYDTNFKMNPPLREESDRQALLEGLLDGTLDCIATDHAPHTAIAKQVEFDR
                     APMGVTGLETALSICLTDLVKPGIVPLDTVLRALGPHPSALLKEPWTPIQEGSAADLV
                     AVDLKRTWTVGQDGFASKSSNSSFLGRELNGVTLLTLVDGRVAWSDPSVVPTNRPAVR
                     VS"
     gene            1780..3291
                     /locus_tag="VF720_03440"
     CDS             1780..3291
                     /locus_tag="VF720_03440"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HEX7878434.1"
                     /translation="MNPRPQGPLAALRPHDALLALEDGTVFPGWSCGAPGEMSGELVF
                     NTSMMGYPEIITDPSYRGQIVTFTYPLIGNYGINAADAESPKPCLSGVVVRTMCGEPS
                     SWRSKETLPDYLRRHRVVAIEGVDTRALTRRLRSAGVMRAVLSTKDLDPASLVAKAKA
                     SPGLVGRDLVAEVTPFEATPWLAAAERPALPVAVLAELPAGTDRFVTDDAIAARLREL
                     PPEIELTDDDILLDETARVDVAAMAVEAAIEARRSGGPMVLPTVETRLKRPSARSEAE
                     RPTTGEELGASGSNTELAVANSHGELAPRIAILHCGMKANIAEELRKRGARVRVWPAA
                     TPIATILADKPDGLLLSNGPGDPEGVDGVVEEVRAALGQVPIFGICLGIQMLALAFGG
                     KTYKLKFGHRGGNQPVLDLTTGKVEITAQNHGFAVDPESLKTGDTSDRRHGLSITHIN
                     LNDGTVEGFQHPGLDVVAVQYHPEAAPGPHDAAYLFDRFLERVRTRMARTGVN"
     gene            3291..>4224
                     /gene="carB"
                     /locus_tag="VF720_03445"
     CDS             3291..>4224
                     /gene="carB"
                     /locus_tag="VF720_03445"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853338.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEX7878435.1"
                     /translation="MPRSDDLKSILIIGSGPIIIGQACEFDYSGTQACQVLKEEGYRV
                     LLVNSNPATIMTDPEIADATYIEPITPDVVERIIAKERPDALLPTLGGQTALNTAVAL
                     AESGVLDKYGVKLIGANLAAIKRAEDREEFRKTMQGIGMDLPLSRQVASLAEAETFAA
                     EAGLPLVVRPSFTLGGLGGGMARTMDDLLSIVAGGLAASPVGTVLLEESVEGWKEFEL
                     EVMRDVKDNVVIICSIENFDPMGVHTGDSLTVAPAQTLTDREYQKMRDDARAIIRAIG
                     VETGGSNIQFAVNPLDGRQVVIEMNPRVSRSSALA"
BASE COUNT          690 a         1495 c         1409 g          630 t
ORIGIN      
        1 tggtgtgcgg cccgaccacg ctgatgccgg tcgacgtgga gtcgatgggg gtcaccgtgt
       61 gccaccgtgt cgaggaggcg ctcgagggag cgcacgtggt gaacatcctg cgcatccagc
      121 aggagcgcct cgaatcgaac ctcctgccga gcctgcgcga gtacgccctc cagttcagcg
      181 tcacgcacga tcgcctccgg gtggcccggc ccgacatcct gatcatgcac ccggggccga
      241 tgaaccgcgg catcgagatc gcccaggacg tggccgagga cgagcgcaac gtcatcctcg
      301 accaggtcac caacggcgtg gccgtccgca tggctgtcct ctaccacctg tcgggcggcg
      361 agtcgctgct gcccgtcggc atcgaaggag ttgcctgatg agttcccgat cgtttcagcg
      421 cctggcgctg tgggacagcc acgaccggcc gcttttcctt tcgggggccg acgtgcgcga
      481 tcccctgaca ggccgcgagg agcggcagga cctctggatc gtgaacggcc ggataacgtt
      541 ccggaagccc gagggaacga cgcccgtggc gttcgacctg accggcgccg tcctcttccc
      601 gcggctgatc gacgtgcacg tgcatctccg cgaacccggt ttcgaggaag ccgagaccgt
      661 ggccaccggc gccctggccg ccgtgaaggg gggcttctcg gccgtctgct gcatgccgaa
      721 caccaacccg ccgctggatg atgccgccac gatccgcttc gttctcgaac aggccgcccg
      781 ggccggcggc gccaccgtgt accccttcgc ctgcatcacc cgtgggcgca agggagaggc
      841 gctggccgac atgttcgaac tggccgaggc cggcgcccgc ggcttctcgg acgacggaag
      901 cccggtcatg aatgccgagc tcatgcggcg ggccctcgaa tactcgacga tgctcgacct
      961 gccgatcgtg gcccacgagg aggattcgca cctggccgca cacggctgcg tgcacgaaag
     1021 ctccgtggcc acccgcgtcg gtctcaaggg aatcccccgc gaggccgagg acgtgatggt
     1081 ggcgcgcgat atcgccctcg tgcgattgac cggcgggcgg ctccacatct gccacgtgtc
     1141 gtcgaaacac acggtggacc tcgtccgcca ggccaaggcg gaggggctgc cggtcaccgc
     1201 cgaggtcacg ccccaccacc tggtgctcac gcacgaacgg gtggccacct acgacaccaa
     1261 cttcaagatg aacccgccgc tgcgcgagga atccgaccgc caggcgctgc tcgaagggct
     1321 gctcgacggc acgctcgact gcatcgccac cgaccacgcc ccgcacaccg ccatcgccaa
     1381 gcaggtggag ttcgaccggg cgccaatggg cgtcaccggc ctcgagaccg cactctcgat
     1441 ctgcctgacc gacctggtga aaccaggcat cgtgccgctc gataccgtcc tgcgcgccct
     1501 cgggccgcat ccgtcggccc tgctcaagga gccctggacg ccgatccagg aggggtccgc
     1561 ggccgacctc gtcgctgtcg atctcaaacg cacctggacg gttgggcagg acggtttcgc
     1621 ctcgaagtcg tccaactcct ccttcctcgg ccgcgagttg aacggcgtca cgctcctcac
     1681 cctcgtggac ggccgggttg cctggagcga tccctccgtg gtgcccacca accgcccggc
     1741 ggtgcgggta tcgtgagcga tccgttcgag ccccgaccgg tgaacccgcg cccccagggc
     1801 ccactggccg cactccgccc gcacgacgcg ctcctggcac tcgaggacgg caccgtcttc
     1861 ccggggtggt catgcggcgc gcccggcgag atgtcgggtg agctcgtctt caacacgtcg
     1921 atgatggggt atcccgaaat catcaccgat ccctcgtacc gcgggcagat cgtcaccttc
     1981 acctatccgc tcatcgggaa ctatggcatc aacgcggccg acgccgagag cccgaagccc
     2041 tgcctgtcgg gcgtggtggt gcgcaccatg tgcggcgagc cttcgagctg gcgctcgaag
     2101 gaaacgctgc ccgactacct gcgccgtcac cgcgtcgtgg ccatcgaggg ggtcgacacg
     2161 cgcgcgctca cccgcaggct ccgttcggcg ggcgtcatgc gcgcggtcct ctcgacgaag
     2221 gacctcgatc cggccagcct ggtggccaag gccaaggcct cgcccgggct cgtggggcgc
     2281 gacctggtgg ccgaggtgac cccgttcgag gccacaccct ggctggcggc cgccgagcgt
     2341 ccggcgttgc cggtggcggt gctggccgag ctgccggccg gcacggaccg cttcgtcacc
     2401 gacgacgcca tcgcggcgag acttcgcgaa ctcccgcccg agatcgagct caccgatgac
     2461 gacatcctgc tggacgagac ggcccgggtg gacgtggccg ccatggccgt cgaggcggcc
     2521 atcgaggcac gacggtcggg tggtccaatg gtgctgccga ccgtggagac gcggctgaag
     2581 cggccatcgg cgcgatccga ggccgaacgc cccacgacgg gggaggagct tggtgcgtcg
     2641 gggtcgaaca ccgagcttgc cgtcgccaat tcgcatggcg aattggctcc tcgtattgcc
     2701 atcctccact gcggcatgaa agccaatatt gccgaggagc tccggaagcg cggcgcccgc
     2761 gtgcgcgtct ggccggcggc cacgccgatc gccaccatcc tggccgacaa gccggacgga
     2821 ctgctgttgt cgaacggccc gggcgatccc gaaggggtgg acggcgtggt cgaggaggtg
     2881 cgcgcggcgc tcggacaggt gccgatcttc ggcatctgcc tcggcatcca gatgctggcg
     2941 ctggcattcg gcgggaagac ctacaagctc aagttcggcc accgcggcgg caaccagccg
     3001 gtgctcgacc tgacgaccgg caaggtggag atcacggcgc agaaccacgg cttcgcggtg
     3061 gatccggagt cgctcaagac cggcgacacc tccgacaggc ggcacgggct ctccatcacg
     3121 catatcaacc tcaacgacgg caccgtcgaa ggcttccagc acccggggct cgacgtggtg
     3181 gccgttcagt atcaccccga ggcggcgccc ggaccgcatg atgccgccta cctcttcgac
     3241 cgattcctcg aacgcgtgcg tacccgcatg gccagaaccg gagtgaacta gtgccccgtt
     3301 ccgacgacct gaaatcgatc ctcatcatcg gctccgggcc gatcatcatc ggccaggcgt
     3361 gcgagttcga ctactcgggc acgcaggcct gccaggtgct gaaggaggag ggatatcgcg
     3421 tcctcctcgt gaattcgaac ccggccacca tcatgaccga ccccgagatc gcggacgcca
     3481 cctacatcga gccgatcacg cccgacgtgg tggagcggat catcgcgaag gagcggccgg
     3541 acgccctcct gcccaccctc ggcggccaga ccgccctcaa caccgcggtg gcgctggccg
     3601 aaagcggtgt gctcgacaag tacggcgtga agctgatcgg cgccaatctc gcggccatca
     3661 agcgtgccga agaccgcgag gagttccgga agacgatgca ggggatcggc atggacctgc
     3721 cgctctcgcg ccaggtggcg agcctcgccg aggccgagac ttttgccgcc gaggcgggac
     3781 tgccgcttgt cgtgcgcccc tcgttcacgc tcggcggcct gggcggcggc atggcccgca
     3841 cgatggacga cctcctctcg atcgtggcgg gcggcctcgc ggccagcccg gtgggcaccg
     3901 tcctcctcga ggagtcggtg gaagggtgga aggaattcga gctggaagtg atgcgcgacg
     3961 tgaaggacaa cgtcgtcatc atctgctcga tcgagaactt cgatcccatg ggcgtgcaca
     4021 ccggcgattc gctcaccgtg gcgccggccc agacgctgac cgaccgcgag taccagaaga
     4081 tgcgcgacga cgcccgggcc atcatccggg ccatcggcgt ggaaaccggc ggctcgaaca
     4141 tccagttcgc ggtgaatccg ctggacggcc gccaggtcgt catcgagatg aatccccgcg
     4201 tcagccggag ctcggcgctg gcca
//
LOCUS       DASVOD010000167         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_950103, whole genome shotgun sequence.
ACCESSION   DASVOD010000167 DASVOD010000000
VERSION     DASVOD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950103"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="VF720_03450"
     CDS             complement(<1..451)
                     /locus_tag="VF720_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004901184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX7878436.1"
                     /translation="MTRAPFDLVSKYAPSGDQPQAIEELVAGLESGQQNQTLLGVTGS
                     GKTFTMANVIARYGRPTLVISHNKTLAAQLYGEFRQFFPNNAVGYFVSYYDYYQPEAY
                     VPSTNTYIEKDASINDDIDRLRLAATTSLLSRDDVVVVASVSCIYGLG"
     gene            complement(484..1851)
                     /locus_tag="VF720_03455"
     CDS             complement(484..1851)
                     /locus_tag="VF720_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878437.1"
                     /translation="MSIRWGFVATLVVLVTASSLPAHSAPRPVVANWTGTLDLYGHYA
                     RPGLARTYLANVRVIAGLDGARLFEWTTRDSADTVGSVESTLLRGDSVFVSVNGGRWR
                     LLDERRATQAWLQAVGFFTFRSGTEAGSNRLTETFRTNADPLRQERRITSSWAHPRFG
                     DVDDLVACRFGADRGSPLPQTLEFHIHERDQDWTARLEYQGGGGPHTGLSLAAPDTFD
                     LPGPDENALTGHLTWETIAPGLHLASMDDVHSRSLVVEFADSLAVLESAVSSANGERL
                     IDAIHEKWPDKPIKWFLFSHHHPHYLGGIRAFIAEGATIAVAPKNRDYVAEIAARKFT
                     REPDRLAAAPKALRMAGVDGRFRLAEGTNEMIAVNIAERSTHTDDFLIFWFPRQRVLF
                     ETEQGWFARDGKTVASRRAAMLLQVLDDEKLDVDRIAQSWPFESTDAVMTRSRLAELV
                     AARGK"
     gene            complement(1877..2377)
                     /locus_tag="VF720_03460"
     CDS             complement(1877..2377)
                     /locus_tag="VF720_03460"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7878438.1"
                     /translation="MSHADSSEAAIRLNLAWNAWANRETLASLRAASHVPSRALGVIG
                     HIVGAERLWLRRMGHDSAPLPVWPQLTIDHIDSELRSLSIDWDAWTAGLDEDSLARFV
                     DYTNSKGESFRNTVVDILSHVTHHSSYHRGQIATLMVQAGEVPACTDYIECVRRGFLD
                     GGWPAG"
     gene            complement(2377..2715)
                     /locus_tag="VF720_03465"
     CDS             complement(2377..2715)
                     /locus_tag="VF720_03465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015203382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX7878439.1"
                     /translation="MIRGEVWWANLERPFGSEPGYRRPVVIIQSDKFNQSRIGTVIIA
                     VITSNIELAESPGNIPLKARTADLPRKSVVNVSQLLTVDRRRLTGRLGQLPPERLAAV
                     DAGLRRVLSL"
     gene            complement(2715..2954)
                     /locus_tag="VF720_03470"
     CDS             complement(2715..2954)
                     /locus_tag="VF720_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX7878440.1"
                     /translation="MKTAISLSDGLFRDAERVAKSLGISRSELYQKALTEFLARQDDQ
                     FVTDALNRIYTAENPGTLDPLFEQAQFEILPDEEW"
     gene            complement(3014..>4027)
                     /locus_tag="VF720_03475"
     CDS             complement(3014..>4027)
                     /locus_tag="VF720_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX7878441.1"
                     /translation="TLLSYDGANLVLGGKDGVRIVNRGEVKTVTLGELPGGLVVRPTL
                     VWDLLSQRSGSELLEVSYLTTGIDWHAEYVAVVDADDRNLDLTGWVSIDNHSGATYRN
                     ARLRLVAGDVNRLQPEMPPMPRREMMAMAADAGNAKQFQEESFFEYHLYTLERPATVS
                     DRETKQLALFPQARAAVTKKMTYDGQKDPKKVSVSVEFANSENNGLGMALPKGVVRVY
                     KQAKDGAEEFVGEDRIDHTAKDEKLRLTLGNAFDVVGERTQTDYQRINDNTFKQSVKI
                     EIRNHKTESVPVSIIEHLPGDWDITEKSTDFKKTDAHTVEFPVTVPADGTVTVTYTAR
                     IRY"
BASE COUNT          683 a         1232 c         1364 g          748 t
ORIGIN      
        1 tgccgaggcc gtagatgcag gacaccgacg ccaccactac cacgtcgtcc cgcgagagga
       61 ggctcgtcgt ggccgccagt ctcagccggt cgatgtcgtc gttgatcgag gcgtccttct
      121 cgatgtacgt gttcgtggag gggacgtacg cctccggctg gtagtagtcg tagtacgaca
      181 cgaagtagcc gacggcattg ttgggaaaaa attgccggaa ttcgccgtac agctgggccg
      241 ccagagtctt gttatgcgag atgaccagcg tgggccgccc gtaccgtgcg atgacgttgg
      301 ccatcgtgaa cgtcttgccg gagccggtga cgccaaggag ggtctggttc tgctgaccgg
      361 attcgaggcc ggccaccagc tcctcgatgg cctgcggctg gtcgccggag ggggcgtact
      421 tggaaacgag gtcgaaggga gcgcgtgtca tggggcacga agtatagcgc gtgcccgcgc
      481 ggtctacttc ccccgtgccg cgaccagctc ggccagccgg gaacgcgtca tcacggcatc
      541 cgtgctttcg aaaggccatg actgggcgat gcggtccacg tcgagctttt cgtcgtccag
      601 gacctggagc agcatcgcgg cccgccggct ggccacggtc ttcccatcac gcgcaaacca
      661 cccctgttcg gtttcgaaga gcacccgctg gcgcgggaac cagaaaatga ggaagtcgtc
      721 cgtgtgcgtc gagcgctccg caatgttgac ggcgatcatc tcgttcgtgc cttcggccag
      781 gcggaaacgg ccatcgacgc cggccatgcg gagcgccttc ggcgcagcgg ccaggcgatc
      841 gggctcgcgg gtgaactttc gcgcggcgat ctcggccacg tagtcccggt tcttcggcgc
      901 cacggcgatc gtggcgcctt cggcaatgaa ggcccggatg ccgcccaggt agtgcgggtg
      961 gtggtggctg aagaggaacc acttgatggg tttgtcgggc cacttctcgt ggatggcgtc
     1021 gatgaggcgt tcgccgttgg cgctcgaaac ggcggactcc agcacggcca gcgagtcggc
     1081 gaattcgacg acgaggctgc gggagtggac gtcgtccatc gaggccaggt ggaggccggg
     1141 agcgatggtc tcccaggtga ggtggccggt cagggcgttt tcgtcggggc ccggtaggtc
     1201 gaacgtgtcg ggggcggcga gggacagccc cgtgtgcggg ccgccgccgc cctggtactc
     1261 cagccgcgcc gtccagtcct ggtcccgctc atggatgtgg aactccaggg tctgtggaag
     1321 aggcgagccc cggtcggctc cgaatcgaca ggccacgagg tcatcgacat caccgaagcg
     1381 gggatgagcc caggacgacg tgatgcgccg ttcctggcga agtggatcgg cattcgttcg
     1441 aaacgtctcg gtcagtcggt tactgcctgc ttccgtcccg gaccggaagg tgaagaaccc
     1501 gactgcctga agccaggctt gcgtggcccg tcgttcgtcg agcagtcgcc accgaccacc
     1561 attgacgctc acgaaaacgg aatcgccgcg aagcagcgtc gattccacgg atccgacggt
     1621 gtcggcggaa tcacgtgtcg tccattcgaa caggcgagca ccatcgagcc ccgcaatcac
     1681 tcggacattg gccaggtatg tccgcgccag gccggggcgc gcgtagtgac cgtacagatc
     1741 gagcgtgccc gtccaattgg cgacgacagg tcgcggagcg gagtgcgccg gcaacgaaga
     1801 ggccgtcacg agcaccacca gtgtcgccac gaacccccat ctgatactca ctccaaatcg
     1861 cccttcgaca tcgtcctcat cccgccggcc atcccccatc cagaaacccc cgccgcacgc
     1921 actcgatata gtccgtgcag gccggcacct cccccgcctg caccatcaga gtggcgatct
     1981 ggccgcgatg gtacgaggag tggtgcgtga cgtgcgagag gatgtcgaca acggtattgc
     2041 ggaaggactc tccctttgag ttcgtgtagt cgacgaaccg ggcgaggctg tcttcatcga
     2101 gtccggctgt ccacgcatcc cagtcgatgg agagcgagcg cagttccgaa tcgatgtggt
     2161 cgatcgtgag ctgcggccag acgggaagcg gtgcgctgtc atgccccatg cggcggagcc
     2221 acaatctctc ggcgccgacg atgtgaccaa taacgccgag agcacgggat ggcacatggg
     2281 aggcggcgcg gagggatgcg agcgtttcgc ggttggccca ggcgttccag gcgagattga
     2341 ggcggattgc cgcttccgat gagtcggcgt gggacatcag agggagagga cccgccgaag
     2401 cccggcatcg acggcggcca gtcgttccgg cggcagttgc ccgagccgcc cggtcagtcg
     2461 tcggcggtca accgtgagca gttgcgacac gttcacgacc gactttcgcg gcaggtcagc
     2521 cgttcgtgcc ttgagtggga tgttaccggg tgattcggca agttcgatgt tcgacgtgat
     2581 gacggcgatg atgacggtgc cgatccgact ctgattgaac ttgtccgatt ggatgatgac
     2641 gaccggcctt cgatagcccg gctcggaccc gaacgggcgt tccagattgg cccaccagac
     2701 ttcgccccgg atcatcacca ctcttcgtca ggcaggatct cgaattgcgc ctgctcgaac
     2761 aacgggtcca gggtgcccgg attctcagcg gtgtagatgc ggttcaacgc atcggtgacg
     2821 aactggtcgt cctgacgcgc gagaaattcg gtgagtgcct tctgatacag ctcgctccgg
     2881 ctgatcccaa gcgacttggc gacgcgctcg gcatcgcgga agagtccatc ggaaagtgag
     2941 attgcagtct tcatgataac ggggagtata accatggtta tactccccgt caaggtggtg
     3001 agcgggtagg gccttagtac cggatccgcg ccgtgtacgt caccgtcacc gtgccgtcgg
     3061 ccgggaccgt gaccgggaat tcgacggtat gcgcgtcggt cttcttgaag tcggtcgact
     3121 tttcggtgat gtcccaatcg ccgggcaggt gctcgatgat cgacacgggg acggactcgg
     3181 tcttgtggtt gcggatctcg atcttgaccg actgcttgaa ggtgttgtcg ttgatgcgct
     3241 ggtagtcggt ctgggtgcgc tcgccgacca cgtcgaaagc attgcctagc gtgaggcgga
     3301 gcttttcgtc cttggccgtg tggtcgatgc ggtcctcgcc gacgaactcc tcggcgccgt
     3361 ccttcgcctg cttgtacacc cggacgacac ccttcgggag ggccatgccc aggccgttgt
     3421 tctcggagtt ggcgaactcc acgctgaccg acaccttctt cgggtccttc tggccgtcgt
     3481 acgtcatctt cttggtgacg gcggcccggg cctgggggaa gagggccagc tgcttggttt
     3541 cgcggtcgct caccgtcgcg gggcgctcga gcgtgtagag gtggtactcg aagaacgact
     3601 cctcctggaa ctgcttggca ttgccggcgt cggcggccat cgccatcatt tcgcggcgcg
     3661 gcatgggtgg catctcgggc tgaaggcggt tcacgtcgcc cgccacaagg cgcagccggg
     3721 cgttccggta ggtggcgccg gagtgattgt cgatcgacac ccaaccggtc aagtcgaggt
     3781 tcctgtcatc cgcgtcgacc acggccacgt actcggcgtg ccagtcgatg ccggtggtga
     3841 gatagctcac ctcgaggagc tcgctccccg aacgctggct caggagatcc cacaccaggg
     3901 tggggcgcac cacgaggccg ccgggaagtt cgcccagcgt gacggtcttg acctcgccgc
     3961 ggttgacgat gcgcacgccg tccttgccgc cgagcacgag gttggcgcca tcatacgaaa
     4021 gcagcgt
//
LOCUS       DASVOD010000168         4356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_793319, whole genome shotgun sequence.
ACCESSION   DASVOD010000168 DASVOD010000000
VERSION     DASVOD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793319"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..659
                     /locus_tag="VF720_03480"
     CDS             162..659
                     /locus_tag="VF720_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX7878442.1"
                     /translation="MIQVKVTGLIIDDTSKSPVVILQELEGERYLPIWIGPFEAQAIA
                     NELQGKKFPRPLTHDLLFSAVNSLKAVVQRIVISDLKEKTFFASIILEKGGEVVSLDA
                     RPSDSIALAVRAKSPIFVAEKVFDGEALDHPPAGVDPTAEESAEELRKRLEGLDPEDF
                     GRFTM"
     gene            800..2200
                     /gene="selA"
                     /locus_tag="VF720_03485"
     CDS             800..2200
                     /gene="selA"
                     /locus_tag="VF720_03485"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEX7878443.1"
                     /translation="MTTPRDQLRAIPAVEILLRNPGLDSIRNSFSHMALVRLVRRVLA
                     DVRAEVLEGRLKEAPAEGELGARVVAEAGRLLAPGPVPMINATGVIVHTNLGRAPLSA
                     AALEAVATAAAGYSNLEYDLVRGVRGSRQSHVEGLLAFLTGSETSLAVNNNAAAVLLA
                     LNSLADGRDVLVSRGELVEIGGSFRIPDIMKKSGARLVEVGTTNKTHRKDFENAIGPN
                     TALILKVHASNYRIEGFTAEVPLPEMAEIGAAHGLPVMVDVGSGALVDVSTRGVEKEP
                     LVSECLAQGADLVTFSGDKLLGGPQAGIVVGRSDLVKRLRENPLARCVRLDKLSLAAL
                     SATLRSFLDPENAWRDVPVLAMLARTESELQGSAESLARDLVASGSNRLTAEVVVTQG
                     AVGGGALPLAGLATRAVAVQVQGFTPDRLERRLRQGSPAVLVRIQNDRVLFDMRTISP
                     AERAMLPGLVARAIES"
     gene            2240..>4356
                     /gene="smc"
                     /locus_tag="VF720_03490"
     CDS             2240..>4356
                     /gene="smc"
                     /locus_tag="VF720_03490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEX7878444.1"
                     /translation="MFVDKLEVFGFKSFSQRLELSFGSGISGVIGPNGCGKSNVVDAF
                     RWVLGEQSSKQLRGDKMEDVIFNGTRDIRPMSMAEVHLTLVNDKGLLPVEYDTVTVGR
                     RLYRSGQSEYTINRQPCRLKDVRDLFLDTGMGSHAYSVIERQMVDNVLSDTTGHRRFL
                     FEEASGIMKYKTRKKEALTKLDATQTDLTRLSDIIHEVERETESLKRQVGKAERYREL
                     RGKLKDLELSLYYRRLSDLAARLDSLADSGATLSTAEQEAEAALAAAEGTLEQLRLQA
                     IMADRQLAGAREDLARLDGEITERNHRITVLKEREASTRQRIEAARERIAVLALRREE
                     NEADGIRAEEARKGHEAALAVESERLTALDGRFRELDARFKAQREDVGKKKQLSLDLF
                     QERLKREGDLRQCLAELEQIAAAQAALETEKAGLVARREEIAGRLDEARQAVAAHVAD
                     IEAAETAATGLAERINAAERAAREHHDDEVVAQKAAAGVASNLQVLENLRKSYEGFEP
                     GVRKVMLDHATDVAGTVADLVRIPAEWVEALRPVLGSWLQHVVTHNVEDARRLIRGLT
                     ADEAGYVNFLPLDRLSTRDHHLLPAHPGVLGRAGNFVTAPAEARALVDHLLGDVLVVD
                     TLDRAIELSNDPAWSGWRYVTADGELLDGVRLGGGKGGDRTNDLLERDREIATLTERL
                     GALNADIARHERARQEALGERIVA"
BASE COUNT          744 a         1496 c         1482 g          634 t
ORIGIN      
        1 aagctcgacg agccgaagcg cgcccagaag gccatcggtt cgctcaacac ccgcttcccg
       61 aagtcggacc tgatcccggc cggggagtgg ctcgtggccc atctcgggga tcgcgagcta
      121 ccgctcatct ccttcgatga gttgaaggcc aagaagccgt catgatccag gtgaaggtca
      181 ccgggctcat catcgacgac acctccaaga gtccggtggt catcctccag gagctggaag
      241 gggagcgcta ccttcccatc tggatcggtc cgttcgaggc ccaggccatc gccaacgagc
      301 tccagggaaa gaaattcccg cggcccctca cccacgacct cctcttctcg gcggtgaaca
      361 gcctgaaggc ggtcgtccag cggatcgtca tctccgacct caaggaaaaa accttcttcg
      421 ccagcatcat cctcgagaaa gggggcgagg tcgtctccct ggatgcacgc ccctccgatt
      481 cgatcgccct ggcggtccgg gccaagtccc cgatcttcgt ggccgaaaag gtcttcgacg
      541 gcgaggcact cgatcatccg ccagccggcg tcgacccgac cgcggaagaa tcggcggagg
      601 agctcagaaa acgcctggaa gggctcgatc cggaggactt cggccgcttc acgatgtaga
      661 cttccccgga aggccgcatc gacgggaccc ccggggggtg tggtcccgcg gtcggaatcc
      721 acctggcggg agccgggccc aacccaatat atggaaaagg gttggcccct acccacaaca
      781 tctggtatta atgcccatca tgaccacccc ccgcgaccag ttgcgcgcca tcccggccgt
      841 cgagatcctt ctccggaacc cgggcctcga ctccatccgc aactccttca gccacatggc
      901 gttggtgcgc ctggtgcgcc gtgtcctggc cgacgtgcgg gccgaggtgc tcgaagggcg
      961 cctgaaggag gccccggcgg agggtgagct cggcgcgcgc gtggtggccg aggcgggaag
     1021 gctcctggcc cccggccccg ttccgatgat caacgccacc ggcgtcatcg tgcatacgaa
     1081 cctgggacgc gcgcccctct cggcggcggc gctcgaagca gtggccacgg ccgccgccgg
     1141 ctactcgaat ctcgaatacg acctcgtgcg cggcgtgcgc ggctcgcggc agtcgcatgt
     1201 cgagggactc ctcgccttcc tcacggggag cgagacgtcc ctggcggtca acaacaacgc
     1261 cgcggcggtc ctcctggccc tgaacagcct ggccgacggc agggatgtgc ttgtctcgcg
     1321 gggcgagctg gtcgagatcg gggggagctt tcgcataccc gacatcatga agaagagcgg
     1381 cgcccggctg gtcgaggtgg gcaccaccaa caagacccac cggaaggatt tcgagaacgc
     1441 catcggcccg aataccgcgc tgatcctcaa ggtgcatgcc tccaattacc gcatcgaggg
     1501 gttcaccgcc gaggtccccc tccccgagat ggccgagatc ggggccgccc acggcctccc
     1561 ggtgatggtg gatgtgggaa gcggcgcgct ggtcgacgtc tcgactcgtg gcgtcgagaa
     1621 ggagccgctg gtttccgagt gcctggcgca gggggccgac ctggtcacct tcagcgggga
     1681 caagctgctg ggcggtccgc aggcgggtat cgtggtcggt cgctccgacc tggtgaagcg
     1741 gctcagggaa aacccgctgg cgcgctgcgt gcggctcgac aagctctcgc tggcggccct
     1801 ctccgccacg ctccggtcgt tcctcgaccc ggagaatgcc tggcgcgacg tcccggtgct
     1861 ggccatgctg gcccggacgg aaagtgaact gcagggatcg gccgaatcgc tggccagaga
     1921 cctcgtggcc tcgggctcga atcgcctcac cgcggaagtg gtcgttacgc agggggctgt
     1981 cggtggcggg gccctgccgc tggcgggcct ggccacgcgc gcggtggcgg tgcaggtcca
     2041 gggcttcacg cccgacaggc tggagcgccg gctgcgccaa ggctcgccgg ccgtcctcgt
     2101 ccgcatccag aacgatcgcg ttctgttcga catgagaacc atctcgcccg ccgagcgggc
     2161 gatgctgccc ggcctcgtgg cccgggccat cgaatcgtga ccgcccccgc cgggcgacgc
     2221 tgaaaggaga gtcaccaggg tgtttgtcga caagctcgaa gtgttcggct tcaagtcgtt
     2281 cagccagcgg ctggagttgt ccttcggttc ggggatctcg ggcgtcatcg gcccgaacgg
     2341 gtgcgggaag agcaacgtcg tcgacgcctt ccgctgggtg ctgggcgagc agtcgtccaa
     2401 gcagctccgc ggcgacaaga tggaagacgt catcttcaac ggcacgcggg acatccggcc
     2461 gatgtcgatg gcggaagtgc acctcacgct cgtgaacgac aaggggctcc tgccggtcga
     2521 gtacgacacc gtcacggtcg gccgccgcct ctatcgctcg ggacagtccg aatacacgat
     2581 caaccggcag ccctgccggt tgaaggacgt gcgcgacctc ttcctcgaca ccggcatggg
     2641 aagccatgcc tactccgtga tcgagcggca gatggtcgac aacgtcctct ccgacaccac
     2701 cggccaccgg cgcttcctct tcgaggaggc ctccgggatc atgaagtaca agacgcgcaa
     2761 gaaggaggcg ctcaccaagc tcgacgccac ccagaccgac ttgacgcgtc tctcggacat
     2821 catccacgag gtggagcggg aaacggagag cctgaagcgg caggtcggca aggccgagcg
     2881 ctaccgggaa ctgcgaggca agctcaagga tctcgagctc agcctctact accgccgcct
     2941 ctccgatctg gcggcgcggc tcgactcgct ggccgattcg ggcgccacgc tctccacggc
     3001 cgagcaggag gccgaggccg cactggccgc ggcggaagga acgctcgagc agctgcggct
     3061 ccaggccatc atggccgatc gccagctggc cggcgcgcgg gaagacctgg cacggctcga
     3121 cggcgagatc accgagcgga accaccggat caccgtcctc aaggagcgcg aagcctccac
     3181 ccgtcagcgg atcgaggcgg cccgcgagcg gatcgccgtc ctggccctcc gtcgcgagga
     3241 gaacgaggcc gatggcatcc gcgccgagga ggcccgcaag ggtcatgaag cggccctggc
     3301 cgtcgagtcc gagcggctga ccgcgctcga cggccgcttc cgcgaactcg acgcccgctt
     3361 caaggcccag cgcgaggacg tcgggaagaa gaaacagctt tcgctcgacc tcttccagga
     3421 gcggctcaag cgcgaggggg acctgcgcca gtgcctcgcc gagctggaac agattgccgc
     3481 ggcgcaggcc gcgctcgaga ccgagaaggc gggcctcgtc gcccgacgcg aggagatcgc
     3541 cggacgcctc gacgaggccc ggcaggccgt ggccgcccac gtggcggaca tcgaggcggc
     3601 cgaaacggcg gcgacgggcc tggcggagcg gatcaacgcc gccgagcggg ccgcccgtga
     3661 gcatcacgac gacgaagtgg tggcccagaa ggcggccgcc ggcgtcgcct ccaacctcca
     3721 ggtgctcgaa aacctccgta agagctacga agggttcgaa cccggcgtgc gaaaggtgat
     3781 gctcgaccat gccaccgacg tcgccggcac cgttgccgac ctggtccgga tcccggccga
     3841 atgggtggag gcgctccggc cggtgctcgg ctcgtggctc cagcatgtcg tcacgcacaa
     3901 cgtggaggac gcccggcgcc tgatccgggg cctcactgcc gatgaggcgg gctacgtgaa
     3961 cttcctgccg ctcgatcgcc tgtcgacgcg cgatcaccac ctgctgccgg cgcatcccgg
     4021 cgtcctcggg cgggccggca acttcgtcac cgcgccggcc gaggcgcggg cgctggtcga
     4081 tcacctgctg ggcgacgtgc tcgtggtcga tactctcgac cgggcgatcg aactctcgaa
     4141 tgatcccgcc tggtcgggat ggcgttacgt gaccgccgac ggggaactgc tcgacggtgt
     4201 ccgcctcggc ggcggcaagg ggggcgatcg cacgaacgac ctcctcgagc gcgaccggga
     4261 gatcgccacg ctcaccgagc ggctgggcgc cctgaacgcc gacattgccc gccatgaacg
     4321 ggcgcgccag gaagccttgg gcgagcggat cgtggc
//
LOCUS       DASVOD010000169         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_584672, whole genome shotgun sequence.
ACCESSION   DASVOD010000169 DASVOD010000000
VERSION     DASVOD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584672"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF720_03495"
     CDS             complement(<1..903)
                     /locus_tag="VF720_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX7878445.1"
                     /translation="MKDLLGGKGANLAEMTNAGVPVPPGFTIATSVCNDWSANGRVAG
                     DFAAEQETYLARLEAAMGKKLGHAHDPLLVSVRSGARFSMPGMMDTILNLGLNDKSVE
                     GLAKRTGNPRFAWDSYRRFIQMFGSVVMEIEKNRFEKQLEALKEARGVHLDTDLTADD
                     LKELVATYKKVVHAERGEDFPQEPRRQLDMARDAVFRSWGNPRAVYYRKQNRIPDNLG
                     TAVNVQAMVFGNMGNDSGTGVGFTRNPADGTNEFYGEYLVNAQGEDVVAGIRTPQPIA
                     KLKDEMPGVYDQLREITSRLEKHYR"
     gene            complement(997..2265)
                     /locus_tag="VF720_03500"
     CDS             complement(997..2265)
                     /locus_tag="VF720_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7878446.1"
                     /translation="MARGGRGAKGPELAAIVADAFGRLPEAGEPIELPLDLAVLGGSA
                     GPESARLALVANRSVWDPRKVALTVDFPAPNVESRVPRSRAVCREFAAHHGLRHVFDL
                     NLGIGAQVLLEMGVVTPGLVVGGCGRCLHLMGAAGSFVVGAEPGVLAPALSTGKLSMR
                     MPHLAQVEFIGPAGGLSAFDMGLMAARALEDLSADTNVQYTGRAVDALGMDGRITLVE
                     VGADALRGGFIAADQVTQGFYKGRRDGGDLVTGRPDVNVAIPSRSVELSGAPLLVQGP
                     GPGGPVRPLSDLAGKKIHSAFIGSCAAGRQADLMEAAGVVKRARRIHGDVRLTLAPAT
                     LEVARHAVTSGLYEIFINAGAMLAVSGSSPGMAGGGAVFGEGEIILSTVPYQSPMTDA
                     GRGPEVYRASPLVVAAAAVAGEIRHPADLR"
     gene            complement(2267..3754)
                     /locus_tag="VF720_03505"
     CDS             complement(2267..3754)
                     /locus_tag="VF720_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7878447.1"
                     /translation="MAEKYLNLIGGKWVPAESGEFFENRNPANWEELIGLFPKSGAAD
                     VDKAVAAAKEAAKTWRLVPAPKRAEIIYRAGEIMRSRKEELARDMTREMGKVIAETRG
                     DVQEAIDCAYFTAGEGRRLYGDTTPAELPNKMAMSIRCPIGVAALITPWNFPMAIPSW
                     KAFPALVSGNTIILKPATETPLSSYHFIKVLEEAGMPPGVVNFICGGGSTCGQYLAEH
                     KDVGVVSFTGSSAVGKHLATTTGATLKRLSLELGGKNGQIVMDDADLDLALEGALWGA
                     FGTTGQRCTATSRLILHKSIADGFTKRLVEAAGKLKVGNGLDAAIQMGPAVSEKQRQT
                     VHEYVEIGKKEGARLLLGGAALTDGPHANGWFYQPTIFGDVKPNMRIAQEEIFGPVLS
                     IITVGSFEEAVEVLNGTVYGLSSSIYTKDVNRAMAAIRDIEAGITYINGPTIGAEVHL
                     PFGGVKETGNGHREASHTVLDIFTEWKSVYIDYSGRLQKAQIDNN"
     gene            complement(4043..>4707)
                     /locus_tag="VF720_03510"
     CDS             complement(4043..>4707)
                     /locus_tag="VF720_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878448.1"
                     /translation="INASTCSPGRILRAYVAGGGALWVSGQLVFAAFAVAPNTMCRSN
                     LSYEFGAGGVGINYMGGDFPCDFMQVCGGAFMNVRTNTNVNGLAGVDASGEGAQLGFR
                     ALEIDRTVFTTGSIGLTDAMFTPTFDVTGGLDTLFTHRAPIATSGMHGKVNAFRYHDP
                     DPIPDQGPVAIFGFPLFFLQPGSVESQTGTFHTARLMIDWLRSEQQRYFLTHSAAGHG
                     RN"
BASE COUNT          736 a         1620 c         1528 g          823 t
ORIGIN      
        1 gcggtagtgc ttctccagcc tgctggtgat ctcgcggagc tggtcgtaca cgccgggcat
       61 ctcgtccttg agcttcgcga tgggctgcgg cgtgcggatg ccggccacca cgtcctctcc
      121 ctgggcgttg accaggtact cgccgtagaa ctcgttggta ccgtcggccg ggttgcgcgt
      181 gaagcccacg ccggtgccgg agtcgttccc catgttgccg aacaccatgg cctgcacgtt
      241 gacggccgtg ccgaggttgt cggggatccg gttctgcttg cggtagtaaa cggcacgagg
      301 atttccccac gagcggaaga cggcatcgcg ggccatgtcg agctggcggc gcggctcctg
      361 tgggaagtcc tcccctcgct cggcatgcac caccttcttg tacgtggcca ccagttcctt
      421 gaggtcatcg gcggtgaggt cggtatcgag gtgcacgccg cgcgcttcct tcagcgcctc
      481 cagctgcttc tcgaaccggt tcttctcgat ctccatcacc acgctgccga acatctggat
      541 gaagcggcgg tacgagtccc aggcaaagcg ggggttaccg gtgcgcttcg cgagcccttc
      601 cacggacttg tcgttgagcc cgaggttcag gatcgtgtcc atcatcccgg gcatcgagaa
      661 cctggcgccc gagcgcaccg acacgaggag cggatcatgg gcatggccca gcttcttccc
      721 catggccgcc tcgaggcgcg cgaggtacgt ctcctgctcg gcggcgaaat cgccggccac
      781 gcggccgttg gccgaccagt cgttgcagac gctggtggcg atggtgaatc cgggaggcac
      841 cggcacgccg gcattcgtca tttcggcgag gttggccccc ttgccgccga gaaggtcctt
      901 catgtccgca cggccatcgg cctggcattt gccgaagaaa aatatccgct ggctcaccct
      961 tttcctccgt ccctcgcgtc tatcggattc gtcagttcat ctcaggtcgg ccgggtggcg
     1021 aatttcgccg gccacggccg cggccgccac cacgaggggg ctggcccggt acacctcggg
     1081 gccacgcccc gcgtcggtca tcggactctg gtacggcacg gtggagagga tgatctcccc
     1141 ttcgccgaaa acggcgccgc cgccggccat gccgggcgac gagccggaaa ccgccagcat
     1201 ggcgccggcg ttgatgaaga tttcgtagag gccgctcgtc acggcatggc gcgccacttc
     1261 gagtgtggcc ggcgccagcg tgaggcgcac gtcgccgtgg atgcggcggg ccctcttcac
     1321 cacgccggcc gcttccatga ggtcggcctg gcgtccggcc gcgcacgacc cgataaaggc
     1381 cgagtggatc ttcttgccgg ccaggtcgga gaggggtctt accgggccgc cggggccggg
     1441 tccctgcacg aggagcggcg ccccggaaag ctcgaccgat cgcgacggga tggccacgtt
     1501 cacgtcgggg cggccggtga cgaggtcgcc cccgtcgcgc cgtcccttgt agaacccctg
     1561 cgtcacctgg tcggcggcga tgaagccgcc gcggagagca tcggccccca cctccaccag
     1621 cgtgatgcgg ccgtccatgc cgagggcatc cacggcgcgg ccggtgtact gcacgttggt
     1681 gtcggccgag aggtcttcga gggcccgggc cgccatgagt cccatgtcga aggccgagag
     1741 gcctcccgcc gggccgatga actccacctg ggccaggtgc ggcatccgca tcgagagctt
     1801 gccggtgctc aacgccggcg ccaggacgcc tggctcggcc cccaccacga aggacccggc
     1861 cgcgcccatg aggtgcaggc agcggccgca cccccccacc acgaggcccg gcgtcaccac
     1921 ccccatctcg aggagcacct gggcgccgat tccgaggttc aggtcaaaga catgccggag
     1981 gccatggtgc gccgcgaact cccggcagac ggcccggctg cgcggcacgc gcgattccac
     2041 gttgggtgcg gggaaatcga ccgtgagcgc caccttccgt ggatcccata cggaacggtt
     2101 ggcaacgagc gccaggcggg ccgactcggg gcccgccgac ccgccaagca cggccaggtc
     2161 gagcggcagc tcgatcggct cgccggcctc gggcaggcgg ccgaacgcat cggccacgat
     2221 ggcggccagc tccggcccct tcgcgccgcg tcccccgcgc gccatgtcag ttgttgtcga
     2281 tctgcgcctt ctgaaggcgc ccgctgtagt cgatgtagac cgacttccac tcggtgaaga
     2341 tgtcgagcac cgtgtggctg gcctcgcgat ggccgttgcc ggtctccttc acgccgccga
     2401 acggcaggtg gacctcggcg ccgatggtcg gcccgttgat gtaggtgatg ccggcctcga
     2461 tgtcgcggat ggccgccatc gcccggttca cgtccttcgt gtagatggag gacgaaaggc
     2521 cgtacacggt gccgttcagc acctccaccg cctcctcgaa cgaccccacg gtgatgatcg
     2581 acagcaccgg cccgaaaatt tcttcctggg cgatgcgcat gttcggcttc acgtcgccga
     2641 agatcgtggg ctggtagaac cacccgttgg cgtgcggccc atcggtgagc gcggcgccgc
     2701 cgagcaggag cctggcccct tccttcttcc cgatctccac gtactcatgg accgtctgcc
     2761 gctgcttctc ggagacggcc ggccccatct ggatggcggc atcgagcccg ttcccgacct
     2821 tgagcttgcc cgccgcctcg accagccgct tcgtgaaacc gtccgcgatc gacttgtgca
     2881 ggatcagccg gctggtggcc gtgcagcgct gccccgtcgt gccgaaggcg ccccacaggg
     2941 ccccctcgag cgccaggtca aggtcggcat cgtccatcac gatctggccg ttcttgccgc
     3001 ccagctcgag cgacaacctc ttcaatgtgg ccccggtcgt cgtggcgaga tgcttgccga
     3061 ccgccgacga tccggtgaac gacaccacgc ccacgtcctt gtgctcggcc aggtactggc
     3121 cgcacgtcga gccgccgccg cagatgaaat tcacgacgcc cggcggcatc ccggcctcct
     3181 ccagcacctt gatgaagtga tacgaggaaa gtggcgtctc ggtggccggc ttcaggatga
     3241 tcgtgttgcc cgagaccagg gccgggaagg ccttccacga ggggatggcc atcgggaaat
     3301 tccagggggt gatgagcgcc gccacgccga ttgggcagcg gatcgacatc gccatcttgt
     3361 tgggaagctc ggccggcgtc gtgtcgccgt acagccgccg tccctcgccg gccgtgaagt
     3421 aggcgcaatc aatcgcctcc tgcacgtcac cccgggtctc ggcaatcacc ttccccatct
     3481 cgcgcgtcat gtcacgggcc agctcttcct tgcgcgagcg catgatctca ccggcccggt
     3541 agatgatctc cgcccgcttc ggcgccggca ccagccgcca cgtcttcgcc gcctctttcg
     3601 cggcggccac ggccttgtcc acgtcggcgg cccccgactt cgggaagaga ccgatcaact
     3661 cctcccagtt ggcgggattg cgattctcga agaactcgcc ggactccgcc ggcacccact
     3721 tcccgccgat caggttgaga tatttctccg ccatgacaac gctccaatca cgattcaacg
     3781 ccccgaaggg gctccaattg acgatttcca gccgcttcga tcggccggcg ggagattcta
     3841 ccagatgagg ctgaggacgt cgccctgaca gggcgacgat tgtcaccgga tttggtgtca
     3901 gccgaggcca ggggccattc agggcgttgc ggggaccacc gtgatgtcga ttcgaaaggc
     3961 ttggaatttc gaagttgcgg agcgtcggct cgctcaccct caccctcggc gtcgtggccg
     4021 gccctctcga cgtcgcacca tctcaattcc gtccgtgtcc ggccgctgaa tgcgtcagga
     4081 aatagcgctg ctgttcgctc cggagccagt cgatcatcag ccttgccgtg tggaatgttc
     4141 cggtctggct ttccactgac cctggttgca ggaagaaaag cgggaagccg aagatggcaa
     4201 ccgggccctg gtcggggatg ggatcggggt cgtggtagcg gaaggcgttc acctttccgt
     4261 gcatgccgga ggtggctatt ggagcacggt gggtgaacag cgtgtcgagg ccgcccgtga
     4321 catcgaatgt cggggtgaac atggcgtcgg tcaacccgat cgagccggtc gtgaagacgg
     4381 ttctgtcgat ctcgagcgcc ctgaatccaa gctgggcgcc ctcaccggag gcatcaactc
     4441 cagccaggcc attcacattt gtgttcgtgc ggacgttcat gaaggcccca ccgcacacct
     4501 gcatgaagtc acagggaaag tcgcccccca tgtagttgat tcctacgcct cccgcgccga
     4561 actcgtagct gagattggac cggcacatcg tgttgggcgc cacggcgaat gccgcgaaga
     4621 cgagctgtcc cgacacccac aaggcgccgc caccagccac gtaggctcgc aggatccggc
     4681 cggggctgca ggtgcttgcg ttgatca
//
LOCUS       DASVOD010000170         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_845440, whole genome shotgun sequence.
ACCESSION   DASVOD010000170 DASVOD010000000
VERSION     DASVOD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845440"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..1201
                     /locus_tag="VF720_03515"
     CDS             113..1201
                     /locus_tag="VF720_03515"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX7878449.1"
                     /translation="MPRYVGLDVHSKASVYAIEDEVGQVIGRGSVPTTPEGLRQIRDQ
                     NTLSPGTGVALEAGVVSFFVVDVLRELGLDPVVIDAAEVRAKVSRPRQKSDRRDAEAL
                     AEGLRLGLYRNIVWIPPMAIRLAREVISRRRHFVRLRTMEVNAAKRLVRGWGKVALSR
                     SLQSGSGWTKLLESLKDTPVLQGFVRGHHELWLATQEQIRILDRQLEELTEPYAAELK
                     RLQTVPGVGRVVAATFLAVVGDARRFGSAKQVASYAGIVPTTYQSGSTDHHGHISRRG
                     SAELRSMLCEAGQHARRTTNPLNPYFSKIRARAGYRKAITAVAHRLSRMLYAILRDGT
                     TFELAKAGVEEGQFQKVSVRRYRQVATT"
     gene            complement(1462..1959)
                     /locus_tag="VF720_03520"
     CDS             complement(1462..1959)
                     /locus_tag="VF720_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878450.1"
                     /translation="MTDYRPPDPKPANLWILQSAFLVGLVTYSGFGAVSLMGRGGDST
                     GIFADPDVNTFVGTGLLLVAILIIILATVLPRLMPALDPDRAATADDYLDRAFKQLVF
                     TNALLEAPALLALIAVLLGRSLLLFPLVVIVLSGVAMVAMMPKIRGWIDEYGRRVERE
                     RSGSR"
     gene            1987..3243
                     /locus_tag="VF720_03525"
     CDS             1987..3243
                     /locus_tag="VF720_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEX7878451.1"
                     /translation="MPQPAFGPAILPGMERDAHLMVTGHLIDSGLLSRCLNLIIENGA
                     RYDMKHFEVGRKVEDVSSAHFTIHAADERIDRIVSELVSLGCRLEGDDPVIVKPSEAD
                     KTVPDDFYSTTNHRSEVRLKSGWVPVADQRMDACLVIDEKAGTARCVKLRDIRKGDAV
                     VCGGTGVRVYPPTKAREAQDFVFMANDVSSERRVERMVTAIAQDMKEAKKAGGRIVVV
                     AGPVVVHTGGGTHLGRLIRNGYVHSLLAGNAVAVHDVEHALYGTSLGVTLQSGIPAHE
                     GHMNHMRAINAINKCGTLAGAVEQGVLKTGVIYECVKAGVPFVLAGSIRDDGPLPDTI
                     MDLIEAQTRTAEQLEGADMLLILSTMLHGIGVGNMTPSWVKTVCVDIHPAVVTKLVDR
                     GSAQAVGIVTDVGLFLRLLADELTPA"
     gene            complement(3406..3609)
                     /locus_tag="VF720_03530"
     CDS             complement(3406..3609)
                     /locus_tag="VF720_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878452.1"
                     /translation="MTDPDPEDWPVSFEANARDQRRRMARLSFAEKLDWLESAHELAR
                     HLAGKASGGASVAQDLERTPSDE"
     gene            complement(3602..>3913)
                     /locus_tag="VF720_03535"
     CDS             complement(3602..>3913)
                     /locus_tag="VF720_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878453.1"
                     /translation="VPMDDFANEERRAEWISAKGMLVFSLRSPDHARTAVDLFLEPPF
                     DFEEMYTRGLRLEISPGVSAMIIGYDDLIQMKRRAGRPIDLADLSILEEGRNDGESTN
                     D"
BASE COUNT          722 a         1212 c         1325 g          654 t
ORIGIN      
        1 caactgtggc tgtttcgatg aagaagggtt gccccccgca agaccgcagt aaggtctgcg
       61 gcggaccacg gggtcatcat tgctgagccc caaaaggcag gaggcaaccc atatgccacg
      121 gtacgtcggt ctggatgtgc acagcaaggc ttctgtttac gcgatcgagg atgaggtggg
      181 tcaagtcatt ggccggggat cggttccaac cacccccgaa ggcctgagac agatccggga
      241 ccagaacacg ctttcgccgg gcaccggggt ggccctggag gccggggtcg tctcgttctt
      301 cgtcgtcgat gtcctgcggg agctcggcct ggatcccgtg gtgatcgatg ccgccgaggt
      361 ccgggccaag gtctcgcggc cgcggcagaa gagtgatcgc cgggatgcgg aagcactggc
      421 cgagggactg aggctgggcc tctaccgcaa catcgtgtgg ataccgccga tggcgatccg
      481 cctggcgcgg gaggtcatct cgcggcgccg gcacttcgtg aggctccgca ccatggaagt
      541 gaacgccgcg aagcggctcg tgcgcggctg ggggaaggtg gccctcagcc ggagcctgca
      601 gtcgggatcc ggttggacga agctgctgga gagcctgaag gacacaccgg tgctccaggg
      661 gtttgtccga ggccaccacg agctgtggct ggccacacag gagcagatcc gtattctgga
      721 tcgacagctc gaagagctga ccgaaccgta tgccgcggag ctcaagcgcc tgcagaccgt
      781 tcccggtgtc ggccgcgtgg tggcggctac gttcctggcc gtcgtcggcg atgcccgccg
      841 gttcggctcg gccaagcagg tggcgagcta cgccggcatc gtgccgacca cctaccagtc
      901 cggatcaacg gatcaccatg gtcatatcag ccggcgcggc tccgccgagc tgcgctcgat
      961 gctctgcgag gcgggtcagc acgccaggcg cacaacgaac ccgttgaacc cgtacttctc
     1021 gaagattcgg gcacgggccg gttaccgtaa ggcgatcacg gcggtggccc accgtctgag
     1081 ccggatgctg tacgcgatcc tgagggacgg aacgacgttc gagctcgcga aggccggcgt
     1141 cgaggaagga cagttccaga aggtatcggt ccgccggtac cgccaagtcg cgacgacctg
     1201 agcgttgcca ggaccgtcag cacatcgttc gactctgaag gcgcgcatgt ttgagccggt
     1261 gaccctgtct tgtggttctg gggttccgaa ccccggtggc tgtttggggc ccggctctcc
     1321 aaaacgcgac atggggcact gcgaccccga atagatgact ggacagcgac ttcttcaggg
     1381 tccgggcgga gcttcgccgg aaaggaaaaa gggagaaatt agttgacgcc cttcttcata
     1441 gataaccaca gttgacctcg atcagcgcga cccggacctc tcccgctcca cccgccgccc
     1501 gtattcgtcg atccaccccc ggatcttcgg catcatggcg accatcgcga cgcccgacag
     1561 gacgatcacg accagcggga agagcagcag tgaccgaccg aggagcacgg cgatcagggc
     1621 caggagtgcc ggcgcctcga gcaatgcgtt ggtgaagacg agctgcttga acgcgcggtc
     1681 gaggtagtcg tcggccgtcg cggcgcgatc ggggtcgagt gccggcatga gccgggggag
     1741 caccgtggcc aggatgatga tcaggatggc cacgagcagt aggccggtgc cgacgaacgt
     1801 gttcacatcg ggatcggcga agatgccggt gctgtcgccg ccccgaccca tcaacgagac
     1861 ggccccgaaa cccgagtagg tcaccagccc gaccaggaag gccgactgca atatccagag
     1921 gttggcgggc ttcggatcgg gtgggcggta gtcggtcatg gcgcccagtc tgcccaagat
     1981 cgccggatgc cgcaaccggc cttcggccct gctatccttc ccggcatgga acgcgacgcg
     2041 cacctcatgg tgaccggaca cctcatcgac tccggactcc tctcccgctg cctgaacctg
     2101 atcatcgaaa acggcgcccg gtacgacatg aagcactttg aggtcggccg caaggtggaa
     2161 gacgtttcca gcgcccactt cacgatccat gccgccgacg agcggatcga ccggatcgtg
     2221 tcggagctcg tgtcgctcgg atgccgtctc gagggggatg atcccgtcat cgtgaagccg
     2281 agcgaagccg acaagaccgt accggacgac ttttactcca cgacgaacca ccggagcgaa
     2341 gtgcgcctga agagcggctg ggtgccggtg gccgaccaac ggatggacgc ctgtctcgtc
     2401 atcgacgaaa aggccggcac cgcgcgctgc gtgaagctgc gcgacatcag gaagggtgat
     2461 gcggtcgtgt gcggcggcac cggcgtgcgg gtctacccgc cgacgaaggc gcgcgaagcg
     2521 caggacttcg tcttcatggc caacgacgtg tcgagcgaac ggcgcgtgga gcggatggtg
     2581 accgccatcg cgcaggacat gaaggaagcg aagaaggccg gcggacgcat cgtggtggtg
     2641 gctggcccgg tggtcgttca cactggcggc ggcacgcatc tcgggcgact gatccgcaac
     2701 ggctacgtgc attcgctcct ggccggcaac gccgtggcgg tgcacgacgt ggagcacgcg
     2761 ctgtacggca ccagcctcgg cgtgacgttg cagagcggca tcccggcgca tgaaggacac
     2821 atgaaccaca tgcgcgccat caacgccatc aacaagtgcg gcactctggc cggcgcggtg
     2881 gagcagggcg tgctgaagac cggcgtcatt tacgagtgcg tgaaggccgg tgtgccgttc
     2941 gttctcgcgg gctcgatccg cgacgacggc cccctccccg acaccatcat ggacctgatc
     3001 gaggcccaga cccgcaccgc cgagcagctc gaaggcgccg acatgctgct gattttgtcg
     3061 acgatgctcc acggcatcgg cgtcggcaac atgacgccgt cgtgggtgaa gacggtatgc
     3121 gtggacattc acccggcggt ggtgaccaag ctggtggatc gcgggtcggc gcaggcggtg
     3181 gggatcgtga cggacgtggg gttgtttttg cggctgctgg cggatgagtt gacgccggcc
     3241 tgacggcggt cgcgggggcg gcggcgcccc ccgcgttatg tgtcccgcgc gttgtcttac
     3301 aatgtaagac acgcggcggt ggcaataaca aaagggccga catcccgcgc gtcagcgcgg
     3361 tgaggaatat tcggctccgc cagcggcgat cccaaggttg gcaacctatt cgtccgacgg
     3421 agtgcgctcc aggtcctgcg ccacagaggc gccacctgac gcttttccag cgaggtgccg
     3481 cgccaactca tgcgccgatt cgagccaatc gagtttctcg gcaaagctca ggcgcgccat
     3541 gcgacgccgc tggtcacggg cgttcgcctc gaagctgacc ggccagtcct cgggatctgg
     3601 atcagtcatt cgttgattcc ccgtcgtttc gaccctcctc caggatcgac agatccgcaa
     3661 gatcgatcgg gcggcctgca cgccgcttca tctggatgag gtcatcgtag ccgatgatca
     3721 ttgcggaaac tccaggagag atttccagac gtagaccgcg ggtatacatt tcctcgaagt
     3781 cgaatggtgg ttcgaggaag aggtcgactg ccgtccgtgc atggtcggga ctccggagcg
     3841 aaaatacgag catgcccttt gcgctgatcc attcagcgcg tcgctcctca tttgcgaagt
     3901 catccatggg cac
//
LOCUS       DASVOD010000171         5641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_271808, whole genome shotgun sequence.
ACCESSION   DASVOD010000171 DASVOD010000000
VERSION     DASVOD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271808"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..479)
                     /locus_tag="VF720_03540"
     CDS             complement(36..479)
                     /locus_tag="VF720_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878454.1"
                     /translation="MLNKDLREFIELLNSTGVEFLIAGGHAVGFHGHPRFTGDIDIVV
                     RPVAANASRVLEAIRRFGFDDLKLDVSDFTAIGRVIQLGYPPNRIDLLTSLSGVTWQE
                     ADSTAVAADLDGVPVRFLGRSELIRNKRATGRAQDLADLEKLEGN"
     gene            complement(424..618)
                     /locus_tag="VF720_03545"
     CDS             complement(424..618)
                     /locus_tag="VF720_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878455.1"
                     /translation="MERVIRVHDSWAAAEEADRAYYAGLTPQERLVIQLELIRRYQES
                     LPHAEQGFKRVYRVVEFDRR"
     gene            complement(639..923)
                     /locus_tag="VF720_03550"
     CDS             complement(639..923)
                     /locus_tag="VF720_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878456.1"
                     /translation="MCHAARLTHETGSEASGRSGRPKNVQFSFMGLGAEADAGDERRG
                     NAPPRILDETELAAHYAQERRRDIITWLAAGIGVLAIVGLLAWQIMRSTR"
     gene            complement(987..1532)
                     /locus_tag="VF720_03555"
     CDS             complement(987..1532)
                     /locus_tag="VF720_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7878457.1"
                     /translation="MPRRFIALVGLVVAALTGLYLGMSVRSSHEVVEESAPPTTELVT
                     GAAFPDVDLVSEDGSSTSSRTMLGGEGGVVLFLLPGRAPCKDMSTEWQRRLDDGELAD
                     VHFYAVANAPADRIALYKGNNHYSFPIVADTAGVFGREHRVEDFPLLIVVDRNHVIRH
                     QTWDPAELVEMAAVRRMIAGE"
     gene            complement(1592..1795)
                     /locus_tag="VF720_03560"
     CDS             complement(1592..1795)
                     /locus_tag="VF720_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878458.1"
                     /translation="MRKMLAAAGLVFAVTFAIGATLPKAEAVSCRYRCICGVPNKCCT
                     NNGVETCKPVSNAPIQCPQSFPC"
     gene            1997..4699
                     /locus_tag="VF720_03565"
     CDS             1997..4699
                     /locus_tag="VF720_03565"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX7878459.1"
                     /translation="MSPAWPIGAPLRALSIAILALVATPLTLFANQSTSPDSTKPSER
                     PPTRRYDTRRCDTPPVVDGDLSDPCWEAVEWGTDFMGWEPQDGVPPSQQTAFKILYTD
                     DALYLAYRAYDTDPERIANRLARRDWFPGDWVEINIDSRYDKRTAYSFTSSVSGTRGD
                     EFVSNDGDNWDGSWDPIWDLETKVDDQGWTAEVRIPFSQLRFSEAEEQVWGIQVTRRL
                     FREEARSLWQPFRREDPGWVSRFGELHGLKNLGSARRLEVFPYMVAKAEKYPEEPGNP
                     FADGADGELNGGLDAKMGITNDITLDATINPDFGQVEADPSEVNLTAFETFFQEKRPF
                     FIEGKNITDFPIAPAITGGSFTQDNLFYTRRIGRNPIYTPDLEEGEYTDQPENTSIIG
                     AAKVSGKTPGGMSIGVLESVTAREQAEIDGPSGPSKEEVEPLSNYFVGRLQQDYGQGE
                     SQVGGMFTSVNRRIEDEHLEFLHDSALSGALDGTHRWGNRKWYITGDAAYSRVSGDPA
                     ALVRTQRTSARYFQRPDNTYRDVDSTATSLAGTGGSVRFGRGGGQGIRFQVGGAWRTP
                     GFEINDIGFMRRADEINEFAWGSYREGNPFSIFRNASINVNQWLTWDFGGNLNIRQFN
                     TNGNAQFKNNWRAGAGTTRLLEYSSNYELRGGPSFLLPGTQDVNVWLDTDTRKRLYFN
                     LSTYHSFGNEDVSENHNYYLGATVEPSNALRISANAEYSDNRNDIQYVSTTGASDGAR
                     YILGKLEQETLALTFRIDYTLTPNLTVQYYGSPFISAGGYTGFRRVTDNRAPDFDDRT
                     HQFADNEIAYDEATDRYLVDEDANGTTDYAFRDPSFNVQDFNSNLVTRWEFSPGSVLF
                     LVWSQARSGFVPHGMFDAGEGLNTLFDQPANNVFLVKVSKWFPM"
     gene            complement(4736..>5641)
                     /locus_tag="VF720_03570"
     CDS             complement(4736..>5641)
                     /locus_tag="VF720_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7878460.1"
                     /translation="GGALQISGAQSVTIQDCAFARNLSAAGLFNDSSGGAIDLVAASV
                     SIVRCAFTDNTAEGTLCGGSGGAVMVGGDAVTFTDCEFRGNSAADGGAVLAVGTTTFD
                     GCRFIGNTASAGGGFCGIPAVSSVLHLAFGGEVRDCVFLNNTGPGSAVAMTGGSVNVV
                     EHSTIAWNGVSSASAQPAVDLRGLDASSVDFRQNVVAFNRGGGIAHAVPASDDVACNI
                     IWANNQDFVAGADWRGQFDNQASDPLFCSQSEPGISVAANSPCLAGGSGHAEGCGVIG
                     TVEVGCPSVSVTPTTWSAIKARIHG"
BASE COUNT         1049 a         1996 c         1715 g          881 t
ORIGIN      
        1 gggctgtcaa tacgacgggg cgacgccggg aggcgtcagt tcccttccag cttctccagg
       61 tcggccaggt cctgtgcgcg acccgtggcc cgcttgttgc ggatcagctc actccgcccg
      121 aggaaccgca ccgggacgcc gtccagatcc gcggcaacgg ccgtcgagtc ggcctcctgc
      181 cacgtcacgc cagaaaggga tgtcagcagg tcgatgcgat tcggcgggta tcccagttgg
      241 atgactcgac cgatggcggt gaagtcggag acgtcgagtt tcaagtcatc aaatccgaac
      301 cgtcgaatcg cttcaaggac ccgggatgca ttcgccgcca cgggcctgac gacgatgtcg
      361 atgtcgcccg tgaaccgggg atgtccgtga aatccgacag catgcccccc ggcaatgagg
      421 aactcaacgc cggtcgaatt caacaactcg ataaactctc ttaaatcctt gttcagcatg
      481 cggcagcgat tcctggtaac ggcggatcaa ttccagctgg atcacgagcc gctcctgcgg
      541 cgtcaggccg gcatagtacg ccctgtcggc ctcctcggcc gcggcccacg agtcgtggac
      601 ccggatgacg cgttccattc atcaatttta tcatgcccct accgggtcga ccgcatgatc
      661 tgccacgcca agagccccac gatcgccaga acgccgatcc ccgccgccag ccaggtgatg
      721 atgtcgcgcc gccgctcctg ggcgtaatgg gccgcgagct ccgtctcgtc caggatccgc
      781 ggcggagcgt tgccacggcg ctcgtcgccg gcgtcggcct cggcgcccag gcccatgaag
      841 ctgaactgga cgttcttcgg ccgccccgat cggccggacg cttccgaccc ggtctcgtgt
      901 gtcagccgcg cggcgtggca cacggcacac cgggcccggg aattgaggtt tgtctcaccg
      961 caatgacagc gccacatggg gcgattctac tcccccgcga tcatccggcg gaccgccgcc
     1021 atctccacca gctcggccgg atcccaggtc tggtgccgga tgacatggtt tcgatccacg
     1081 acgatgagga gcggaaaatc ctccacgcgg tgctcgcgcc cgaagacgcc ggccgtgtct
     1141 gccacgatcg ggaaggagta atgattgtta cccttgtaga gggcaatccg gtcggccggg
     1201 gcattcgcca ccgcatagaa gtgcacgtcg gccagttcac cgtcgtccaa ccgccgctgc
     1261 cactcggtgg acatgtcctt gcagggcgcg cggccgggca ggaggaacag gacaacccca
     1321 ccctcgcccc cgagcatcgt ccggctcgac gtcgacgaac catcctcgga aaccaggtca
     1381 acatcgggga aggccgcgcc ggtcacgagc tccgtcgtcg gcggcgcgct ctcctcgacc
     1441 acctcgtgcg aggagcggac cgacatgccg agatagaggc cggtcagggc ggcaacgacc
     1501 aggccaacga gggcaatgaa tcggcggggc atgggaaggg ggcaaattcg acacgggggg
     1561 ccggcgcccg gccccccgcg ccggattcaa ctcagcaggg gaagctctgg ggacactgga
     1621 tcggggcatt cgataccggc ttgcaggtct cgacgccgtt gttcgtacag cacttgttgg
     1681 ggacaccaca gatgcaccga tagcggcacg aaaccgcctc ggccttcggc agcgtggccc
     1741 cgatcgcgaa cgtgacggcg aataccaggc ctgcggcagc gagcatcttg cgcattcgga
     1801 cctcctgttg gggtggcagt cggccgggag gatagaaaac tccattggcg gcccgacgtc
     1861 aagaaggtcg aaatccgcta ttttcaggca acggttgtcc atgtccgggc gtctatgacg
     1921 cggtgccgat ggaactgccg cggccgcccg gttcgggccg gtcgatccca tcgaaagatt
     1981 ccggggattt cgacacatgt cgcctgcatg gccaattggc gcgcctctcc gcgcgctctc
     2041 gatagcgatc cttgccctcg tcgcaacgcc gctgaccctg ttcgccaatc agtcaacatc
     2101 gcccgattcc acgaagccct ccgaacgccc ccccacgcgc cgctatgaca cccggcgctg
     2161 cgacacgccg ccggtcgtcg atggcgatct ttccgatccc tgctgggagg cggtggaatg
     2221 gggtaccgac ttcatgggat gggagccgca ggacggtgtt cccccgtcgc agcagaccgc
     2281 cttcaagatc ctctacacgg acgacgcgct ttacctggcc taccgcgcct acgacaccga
     2341 ccccgagcgc atcgccaatc gcctggcccg tcgtgactgg ttcccgggag actgggtcga
     2401 gatcaacatc gacagccgct acgacaaacg caccgcctac tccttcacct ccagcgtctc
     2461 cggcacgcgc ggcgacgaat tcgtcagcaa cgacggcgac aactgggacg gcagctggga
     2521 cccgatctgg gatctcgaga cgaaggtcga cgaccagggg tggaccgccg aggtccgaat
     2581 ccccttcagc cagctccggt tttccgaggc ggaggagcag gtctggggta tccaggtcac
     2641 ccgtcgcctc ttccgcgagg aagcgcgctc cctctggcag ccgttccgtc gcgaggatcc
     2701 gggttgggtg agtcgtttcg gggagctgca cgggctcaag aacctgggct cggcccgccg
     2761 actggaggta tttccctaca tggtggcgaa ggccgagaaa tatccggagg agccgggcaa
     2821 ccccttcgcc gacggcgccg atggcgagct caacgggggc ctcgacgcga agatgggaat
     2881 caccaacgac atcacgctgg acgccacgat caatcccgat ttcggacagg tggaagccga
     2941 cccgtccgag gtcaacctga ccgccttcga gaccttcttc caggaaaagc gtcccttctt
     3001 catcgaggga aagaacatca ccgacttccc catcgcgccg gccatcaccg gcggctcgtt
     3061 cacgcaggac aacctcttct acacgcgccg catcggccgc aacccgatct acacccccga
     3121 tctcgaagag ggggaataca ccgaccagcc ggagaacacc tcgatcatcg gcgcggccaa
     3181 ggtcagcggc aagacgccgg gaggaatgtc gatcggcgtg ctggaaagcg tgaccgcccg
     3241 cgagcaggcc gagatcgacg gcccgagcgg ccccagcaag gaagaggtgg agccgctcag
     3301 caactacttc gtcggccgcc tccagcagga ctacggccag ggggaatcgc aggtcggcgg
     3361 catgttcacc tcggtcaatc gacggatcga agacgagcat ctcgagttcc tgcacgactc
     3421 ggccctctcg ggagcgctcg acggcacgca ccggtggggc aaccggaagt ggtacatcac
     3481 gggcgacgcg gcctacagcc gcgtatcggg cgatccggcc gcgctggtgc gcacgcagcg
     3541 cacgtcggcc cgctacttcc agcgtcccga caacacctac cgtgacgtcg actccaccgc
     3601 cacttcactt gccggaaccg gcggttccgt ccgcttcggc cgcggcggcg gccaggggat
     3661 ccgcttccag gtgggcggcg cctggcgcac gcccggtttc gagatcaacg acatcggctt
     3721 catgcgccgc gccgacgaga tcaacgaatt cgcatgggga agctaccgcg aagggaaccc
     3781 gttctcgatc tttcgcaacg cttcgatcaa cgtcaaccag tggctcacct gggacttcgg
     3841 cggcaacctc aacatccggc agttcaatac caacggcaat gcccagttca agaacaactg
     3901 gcgcgccggc gccggcacca ccaggctcct cgaatactcg agcaactacg agctgcgggg
     3961 cggcccgagt ttcctcctgc ccggcacgca ggacgtgaac gtctggctcg acaccgacac
     4021 ccgcaagcgc ctttacttca acctgagcac ctaccacagc ttcggcaatg aggacgtgtc
     4081 ggagaaccac aactactacc tcggagccac cgtcgagccg agtaacgccc tccggatcag
     4141 cgccaatgcc gagtacagcg acaaccggaa cgacatccag tacgtctcga ccacgggcgc
     4201 gagcgacggc gcccgctaca tcctcggcaa gctcgagcag gaaaccctgg ccctcacctt
     4261 tcgcatcgac tacacgctca caccgaacct cacggtccag tactacggct ccccgttcat
     4321 ctccgccggc ggttacaccg gcttccgccg cgtcaccgac aaccgcgccc ccgacttcga
     4381 cgaccgcacc caccagttcg ccgacaacga gatcgcctac gacgaagcca ccgaccgcta
     4441 cctggtggac gaagatgcca acggcaccac cgactacgcc ttccgcgacc ccagcttcaa
     4501 cgtccaggac ttcaattcca acctggtcac ccgctgggaa ttctcgccgg gctcggtgct
     4561 cttcctcgtc tggtcacagg cgcgcagcgg cttcgtgccc cacggaatgt tcgacgccgg
     4621 cgaaggcctg aacacgctgt tcgaccagcc ggccaacaac gtcttcctgg tcaaggtgag
     4681 caagtggttc ccgatgtagc cacctggctt ccatcccccg gccgtcccgg caacgtcacc
     4741 cgtgaatccg cgccttgatc gcactccacg tcgtcggcgt cacgctgaca gacggacatc
     4801 ccacctccac cgtgccgatc acgccgcacc cttccgcgtg acccgatcct cccgccaggc
     4861 agggggaatt ggccgccacg gagatgccgg gttccgactg cgagcagaac agcggatcgc
     4921 tggcctggtt gtcgaactgg ccgcgccagt cggcgccggc cacgaagtcc tggttgttgg
     4981 cccagatgat gttgcaggcc acgtcgtccg aggccggaac cgcgtgggcg atgccgcccc
     5041 cccggttgaa ggccacgacg ttttgcctga agtccaccga ggaggcgtcg aggccgcgca
     5101 ggtcgacggc cggctgggcg ctcgccgacg acacaccatt ccaggcgatc gtcgagtgtt
     5161 ccacgacgtt cacgctcccc cccgtcatgg ccactgccga acccggtccg gtattgttga
     5221 ggaacacgca atcccggacc tctcccccga acgccaggtg gagcacggag gacaccgcgg
     5281 gaattccgca gaagcccccc ccggcgctcg ccgtattccc gatgaaccgg cacccgtcga
     5341 acgtcgttgt cccgacggcc agtacggcgc cgccgtccgc ggccgagttg ccgcggaact
     5401 cgcagtcggt gaacgtgacg gcatcgcccc ccaccatcac cgcaccgccc gagccgccac
     5461 agagcgttcc ctcggcggtg ttgtccgtga acgcgcagcg cacgatggag acgctggcgg
     5521 ccaccaggtc gatcgcgccg cccgagctgt cgttgaagag gcccgccgcc gacaggtttc
     5581 gcgcgaaggc gcagtcctgg atcgtgacgc tctgcgcgcc ggagatctgg agcgcgccgc
     5641 c
//
LOCUS       DASVOD010000172         3511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_168046, whole genome shotgun sequence.
ACCESSION   DASVOD010000172 DASVOD010000000
VERSION     DASVOD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168046"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VF720_03575"
     CDS             <1..1071
                     /locus_tag="VF720_03575"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX7878461.1"
                     /translation="TVLSTYFRAWIVFIPVQIFLPRQWDVPGVIPYPGGFTLGWMLFL
                     NLVTAHAVRFKWQWKRAGLILIHLGLLLLIVGEFVTAISAEENRMTIVEGGTVNYATD
                     IREVELAVVDRSAPDVDQVVAIPGHRLAHDQVIRDPRLPFDVRVEAYYQNAELVEGVD
                     RAGGAGQADMGYAASRAITLRPVPPASGTDRDRSDLPAAMVTLEKDGKRLGTWIVSLY
                     LTLTDVPPQPVTVDGRTVDMALRFKRVYKPYSMSLIDFRHDTYPGTETPMNYSSDVRL
                     VDAAHGEDRQVRIYMNNPLRYRGETFYQSSFLEGDTGTVLQVVRNPGWLLPYLACAIG
                     AFGLMLHFGTRLMAFLRRRSAA"
     gene            1068..2825
                     /gene="ccsA"
                     /locus_tag="VF720_03580"
     CDS             1068..2825
                     /gene="ccsA"
                     /locus_tag="VF720_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX7878462.1"
                     /translation="MNRMNSWMSRLPHALLLLSILYVASHMRPDRSDGWNTGQFGRLP
                     VQDLGRVKPIDTLARTSLMVLSNRQSFYTAGESGGSDRKRLRNPHKTPAVRWLLDVVA
                     RPSQARTYEVFRIDHPDVLALIGAPDTRKYFSLNEIMANEDRLLAQFDQTRAIDKNDW
                     TPYQRQLMNLHNKLSLYFTLEDAERLRFVPSPDGQADWLTLRTSDAPGRPTDSSIQYA
                     RSVTAAFIAWRDQDATQFNAAVQAAGLGLGDVPAPRRARLTHEYEFNHMAPFIVAMSL
                     YVFTFVLALLSWLRYSAPLSKASLWILGLALVIHTAGLISRIYIQGRPPVTNLYSSAI
                     FVGWGAAVLGFVLERLYRNGVGTATAASLGFLTLLVAHNLSMDGDTMTMMQAVLDTNF
                     WLATHVVVITLGYGATFLAGFLSILYILRGVFTRGLDQATQASLSRMIYGIIAFATLF
                     SFVGTILGGIWADQSWGRFWGWDPKENGALLIVLWNALILHARWGGMVRHRGIAVLGV
                     AGNIVTSWSWFGTNMLGVGLHAYGFIDSALLWMFIFVASQLAIIAIGLTPPHKWRSYA
                     AKSPAPVPVGPLPVPTRHQ"
     gene            2992..>3511
                     /locus_tag="VF720_03585"
     CDS             2992..>3511
                     /locus_tag="VF720_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878463.1"
                     /translation="MTYETGLDAITELVRWASEHVAERNEATTRLHLIDTLLLKCLGW
                     PMHMVEAEKHFRKEYSDYELGEPSKLLLVEAKREGIYFELPHGFGQEVCRLETLAGLN
                     VGIGDAIKQAMEYAQSRGIPVAAVTNGHQLIAFIASRQDGVPPSKGEAIVFESLEAIR
                     TRFRQFWNCLSPS"
BASE COUNT          608 a         1189 c         1053 g          661 t
ORIGIN      
        1 acggtcctct ccacctactt ccgggcctgg atcgtcttca tcccggtgca gatcttcctg
       61 ccgcgccagt gggacgtgcc gggcgtgatt ccctaccctg gaggattcac gctggggtgg
      121 atgctcttcc tgaacctggt cacggctcac gccgtccgtt tcaagtggca gtggaagcgg
      181 gccgggctca tcctcatcca cctgggactc ctgctgctga tcgtgggcga gttcgtcacg
      241 gccatctcgg ccgaggagaa ccggatgacg atcgtggaag ggggcaccgt caactacgcc
      301 acggatatcc gcgaggtgga gctggccgtg gtcgaccgct cggctcccga cgtggaccag
      361 gtggtggcca tccccggcca tcggctggcc cacgaccagg tgatccgcga tccacggctt
      421 ccgttcgacg tccgcgtgga ggcgtattac cagaacgccg aactcgtgga aggggtggat
      481 cgcgcaggcg gggcgggcca ggccgacatg ggttacgcgg caagccgcgc catcaccctt
      541 cgcccggttc caccggccag cggcaccgac cgcgatcgga gcgacctccc ggcggccatg
      601 gttacgctcg agaaggatgg aaaaaggctc ggcacctgga tcgtgtcgct ctatctcacg
      661 ctcaccgacg tgccgccgca gccggtgacg gtggacggac gcaccgtcga catggcgctc
      721 cgcttcaagc gcgtctacaa accctattcg atgagcctga tcgacttccg ccacgacacc
      781 tatccgggga ccgagacgcc catgaattat tccagtgacg tgcgcctggt cgatgccgcc
      841 cacggcgagg accggcaggt gcgcatctac atgaacaatc cgctccgata ccgcggcgaa
      901 acgttctacc agtcgagctt cctggaaggg gacaccggca cggtgctgca ggttgtgcgc
      961 aacccgggtt ggctcctccc gtatctggcc tgcgccatcg gggccttcgg gctgatgctc
     1021 cacttcggca cccgcctcat ggcgttcctc cgccgccgga gtgccgcatg aaccgcatga
     1081 attcgtggat gtcgcgtctc ccgcacgcgc tgctgctcct ttccatcctg tacgtggcct
     1141 cccacatgcg tcccgacagg agcgacggct ggaacaccgg gcagttcggc cggctccccg
     1201 tgcaggacct ggggcgcgtc aagccgatcg acacgctggc ccgcaccagc ctgatggttc
     1261 tctcgaaccg gcagtcgttc tacaccgcgg gcgagagcgg cggatcggat cggaagcgcc
     1321 tgcgcaatcc ccacaagacg ccggccgtcc gctggctgct cgacgtggtg gcccgcccct
     1381 cgcaggcgcg cacctacgaa gtgttccgga tcgatcaccc cgacgtgctg gccctgatcg
     1441 gcgcgcccga cacgcggaaa tatttctcgc tgaacgagat catggccaac gaggatcggc
     1501 tcctcgcgca gttcgaccag acccgcgcca tcgacaagaa cgactggacg ccgtaccagc
     1561 gccagctcat gaacctccac aacaagctct cgctttactt cacgctggaa gacgcggagc
     1621 ggcttcgttt cgttccgtcg ccggatggcc aggccgactg gctcacgctg cgcacgtccg
     1681 acgcaccggg ccggccgacc gactcctcga tccagtacgc gcgctcggtc accgccgcct
     1741 tcatcgcctg gcgtgaccag gacgccaccc agttcaacgc cgccgtccag gccgccggcc
     1801 tcggcctggg cgatgtgccg gctccgcgcc gggcccgcct cacgcacgaa tacgagttca
     1861 accacatggc ccccttcatc gtggccatgt ccctctacgt cttcacgttc gtcctggccc
     1921 tcctctcgtg gctccgctac agcgcgccgc tgtcgaaggc ctcgctctgg atcctggggc
     1981 tggcgctcgt catccacacg gcggggctca tctcgcgcat ctacatccag ggtcggccac
     2041 cggtcaccaa cctctactcg tcggccatct tcgtcggatg gggcgccgcc gtgctcggct
     2101 tcgtcctgga gcgcctctac cggaacggcg tgggaaccgc cacggccgcc agtctcggct
     2161 tcctcacgct gctggtggcc cacaacctgt cgatggacgg cgacacgatg acgatgatgc
     2221 aggccgtcct cgacaccaac ttctggctgg ccactcacgt cgtcgtcatc acgctcggct
     2281 acggcgccac gttcctggcg ggcttcctgt cgatcctcta catcctgcgc ggcgtcttca
     2341 cgcgaggcct cgaccaggcc acccaggcca gcctctcgcg catgatctac ggcatcatcg
     2401 ccttcgccac gctcttctcg ttcgtcggca ccatcctcgg cggcatctgg gccgaccagt
     2461 cgtgggggcg cttctggggc tgggatccca aggaaaacgg cgcgctcctc atcgtcctct
     2521 ggaacgccct gatcctccac gcccgctggg gcggcatggt ccgccaccgc ggcattgcgg
     2581 tcctcggcgt ggccggcaac atcgtgacga gctggtcctg gttcggcacc aacatgctcg
     2641 gcgtcgggct gcacgcctac ggcttcatcg actcggcgct cctctggatg ttcatcttcg
     2701 tggcgtcgca gctggccatc atcgccatcg ggctcacgcc gccgcacaag tggcggagtt
     2761 acgcggcgaa gtcaccggcg ccggtcccgg tgggaccctt gccagtgccg acgcggcatc
     2821 aatagaatct cgtccacgaa acgcgccgga tctcagcact agcatgctcg gccagaagga
     2881 cggacctcag cttcacaatt cttgaggtgc cgtatcgacc ttcatacgca tataggcctc
     2941 gagtcagtat ccagtacgga agtatgcaag tgataaagcc ggagatgctc aatgacatat
     3001 gagactggtc tcgacgcgat caccgagttg gtacgctggg catctgagca tgtagccgag
     3061 cgcaacgagg ctacgactcg acttcatcta atcgacacac tcttattgaa atgcttgggt
     3121 tggccaatgc acatggtcga agccgagaag cactttcgga aagagtacag tgattatgag
     3181 cttggagagc cttcgaaact gttgttagtg gaggccaagc gggaggggat ctacttcgag
     3241 cttccacacg gtttcgggca agaggtctgt cgtctcgaga ctttggcagg attgaacgtt
     3301 ggaattggcg atgcaatcaa gcaagcgatg gaatatgcgc agagtcgagg aattccggtc
     3361 gcagcggtga caaacggcca tcaactaatt gccttcattg cttcccgcca ggacggagtt
     3421 cctccaagta agggtgaagc aattgtattt gagtctcttg aggcaatcag aactagattt
     3481 cggcagttct ggaattgcct gtcgcccagt g
//
LOCUS       DASVOD010000173        12618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_115537, whole genome shotgun sequence.
ACCESSION   DASVOD010000173 DASVOD010000000
VERSION     DASVOD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12618
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115537"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2378
                     /locus_tag="VF720_03590"
     CDS             <1..2378
                     /locus_tag="VF720_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7878464.1"
                     /translation="VGRHGEAPIYLADVALVEDGFEDVRRAARVNGEPAQGLGIRKQR
                     GANAVAVGEAVKHEMEEIRKTLPEGMELGINFDSTQFIEESVHEIQFELILAVILTAL
                     VCWIFLGSLSSTLNVILAIPMSLFGTVAVIYFLGYTFNTFTLLGMALAVGIVVDDAIM
                     VLENIHRHGEMGKDKVTAAREGTAEITFAALAATLAVVAIFMPVIFIEGVVGRFFFQF
                     GVTLCVAVLFSYLEAITLAPARCAQMLDVSREKRGKIGRAVDDAFHKLENGYRRLLPL
                     ALNRPVMVLGGATLLFAGAIWILRSLPSEFVPSQDQSRLMVRVQTAVGADLHETDALF
                     EKAEAKVSAHPEVTRIFAVVGGFGGSGVNSGVIFVTLVPPKQRKIGHLDFAAQLRREL
                     NGIPGVRAVVQDPSQQGLTARRGFPVEFSVRGANWDGLVDVSTGLMRDMDASGTVVDL
                     DSDYRVGMPELRIVPDRARCADLGIPVEEVAAAINALVGGIPVGKYSEGGRRMDVRMR
                     LLADQRARPEDIVRLRVRTANGDLVPLSSVVTTEERPALQAITRRDRERAITLTANVA
                     PGHSQQDALKAVEGLAADVPDGYRVVLGGASVAFKESMGSLLFALVMGIIVAYMVLAS
                     QFNSLLHPITVLTILPLSVAGAAGALLLTGKTLNIFSMIGLLLLLGIAKKNSIILVDY
                     GRQIRDQGKNARDAMLEAGPRRLRPILMTSVATMMAAVPPALGLGPGSEIRTPMAIGI
                     IGGIVVSTALSLFVVPTFFVTADKVTEGIKGSMARRKARREGRTEEAATQA"
     gene            complement(2418..3068)
                     /locus_tag="VF720_03595"
     CDS             complement(2418..3068)
                     /locus_tag="VF720_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878465.1"
                     /translation="MHSRFDHLVITAGSLAAGTDWVTKRLGVTPGPGGQHPYMGTHNT
                     LLRLGDDAYLEVLAIDPQAAPPDRPRWFALDDAAMTAEPRLAGWVVRVDDIEAVVALA
                     PVPPGSIETMTRGDLTWRLTIPVDGRPPWGGALPILIEWPEGRHPTTTMTGSGCSLER
                     LDLFHPDVVALRAFLDRIGLECPLVVRPGKSGLQAHVQTPSGTKVMSSGRSVEDQV"
     gene            complement(3205..3471)
                     /locus_tag="VF720_03600"
     CDS             complement(3205..3471)
                     /locus_tag="VF720_03600"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7878466.1"
                     /translation="MSSEPDEVTVLEVADPVQQSLIESVLTDAEVPYMVRHVGVQNLI
                     GAGTLGGHNFLTGPIVIQVMREDAERARELIAAALNVPELPAED"
     gene            complement(3537..3983)
                     /locus_tag="VF720_03605"
     CDS             complement(3537..3983)
                     /locus_tag="VF720_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878467.1"
                     /translation="MLTRTLGLLVALSILGCATERRPESLRATLADGEWRLLVDEQLA
                     APVTGIPGDAIPDSAYRDEPAGPELRVRAGQGATRIEIVDERLVGELTRESTPTSLTY
                     DLVQGTVAGGRFRAWTERDVVRAELTLYGSGVPVLSSRRGKLIKVG"
     gene            complement(3994..4143)
                     /locus_tag="VF720_03610"
     CDS             complement(3994..4143)
                     /locus_tag="VF720_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007530931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878468.1"
                     /translation="MIRKLPSGVYRLYSRRKDPKTGNRRNLGTFKTRAAAEKHERAVQ
                     YFKRK"
     gene            complement(4150..4509)
                     /locus_tag="VF720_03615"
     CDS             complement(4150..4509)
                     /locus_tag="VF720_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019519980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX7878469.1"
                     /translation="MPGLFSYGTLQQENVQIATFGRLLSGTPDTMIGFILAPLAIDDP
                     HVVAVSGKAVHTIAAYDGQETSRIPGTVFEITDDELAAADRYEVEACLRVAVTLASGM
                     RAWVYVDRNRPGPGIQE"
     gene            complement(4537..5439)
                     /locus_tag="VF720_03620"
     CDS             complement(4537..5439)
                     /locus_tag="VF720_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878470.1"
                     /translation="MRIAILTLRALPRFITREVANVDEFFADDRRLIAEFDRFGVSAE
                     AVSWGDPDVDWNSYDIAIIRSTWDYIDARDEFLAVLKRIEASSCRLINPLDTVRWNSD
                     KVYLTELYAQGIPTVPTYRAGLSHPFDLHRLLAEHGWDDAVIKPRVGAAGFGVRRVSL
                     GELRSELQELRAAGVAKDFIVQPLIETIREEGEWSFIFIDGRFSHLVRKRPAAGDYRI
                     NTTYGGTDEVATPRPDHLAEAGAIMARIDFRHLYARLDLVRIGADMVIMEVELIEPFL
                     YFDHAPESVARFVDAVLRPPSTDA"
     gene            complement(5449..6216)
                     /locus_tag="VF720_03625"
     CDS             complement(5449..6216)
                     /locus_tag="VF720_03625"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878471.1"
                     /translation="MTSEMPTMPAGSGQHPVTLVVISANRLDESTGFYSSLFGWKMQP
                     LSGELTAIITPDGPAAALRTRVPAGFPGIVPYLRVSDVEAAMARVVAAGATVERAPWN
                     VAPVGTLARFKDASGTIYGVTNGMAGRPTPAIPVPFGDNPKPLPGSICSLEMYAADGA
                     AAAAFFGEQFGWGSAAMMPQYMSFNPGAGISGVFQSHTPALPAVAYIYVSDVHATIAA
                     IEAAGGKRVGDPMAMPGMATFGYFTDASGTSMGLIGP"
     gene            complement(6302..7333)
                     /locus_tag="VF720_03630"
     CDS             complement(6302..7333)
                     /locus_tag="VF720_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878472.1"
                     /translation="MVRRLSLVIVAAAALLAAGTRTARASDPGTAVPACDLTSATPWV
                     ERWFNAWDLTSREILHIPTEAPPYFVFYDSLCVYTTSAVSAAGAAPIAGPRFQLSELP
                     WRAVAHDDSIRLPDSTRLAVQLMTFAGGSKEHGGVFFVMGSPTFWKSAGHGQEPGLTA
                     VFLHEFAHTRQIPGMAAVMGPLDSAWTFPEPLSDDVVQLRFGRDSTYVAEYLAERDLL
                     YEGARTGTDDEARALAAKALGMMRARHARWFVDADSVFAALDDTFLSLEGAGQWTGQA
                     WLAHPQGGGLTGDEAVSKMLGRRRWWSQDEGLALFLLVDRLVPEWPSMVFRVPSAGAI
                     ELLERAVKP"
     gene            complement(7338..7709)
                     /locus_tag="VF720_03635"
     CDS             complement(7338..7709)
                     /locus_tag="VF720_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7878473.1"
                     /translation="MTRPQLQQWLDDYIGAWRTYDSSRIGALFSTGAEYHFNPFGTEP
                     PVRGRDAIVANWLSERDDPGSWEAHYEPVAVEGNVGVARGRTRYTRDGVLLREFANIF
                     VMEFDEEARCTRFTEHYMERR"
     gene            complement(7811..8455)
                     /locus_tag="VF720_03640"
     CDS             complement(7811..8455)
                     /locus_tag="VF720_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007508897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7878474.1"
                     /translation="MSRLRVLAYSMSLDGFSAGPDQSLENPLGVRGPEIMAWLFPTRM
                     FAVMQKQDGGETGVDNRFAEDSMEMGAWIMGRNMFGPVRGPWPDESWRGWWGPNPPYH
                     VPVFVLTHHPRAPLTMEGGTTFHFVTGGIHEALDRARDAAGGRDIRIGGGVATVRQYL
                     EARLVDDLHLAVGPVLLGRGEHLFHGLDLDALGYACTEAIPGERATHVMITRRG"
     gene            complement(8566..9858)
                     /locus_tag="VF720_03645"
     CDS             complement(8566..9858)
                     /locus_tag="VF720_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7878475.1"
                     /translation="MSLRNRFLLYLVLVHLVFGAAIATLALPKRPWLLGLEAFLLASL
                     LAGIRLVRHLTRPHELTQQALNQLESGDFTTRFIEEGPHDVERLMRVYNAMTERLRRE
                     RILNREQDSLLKRLVQLSPSGMISLDFDGNAVEMNPAAAALLGGGDSLLGTPPAAWSN
                     PLGAELAQLVDGESRLVTLAGRRRLRCLKLPYIDRGFARSFILIDEMTRELYESEKAA
                     YEKLIRTMSHEINNTSASVISLLQSCLDSPADDHWRESLTVAINRTSHMNAFLRRFAD
                     VIRLPPPHTESCNVNEMLTETARVLKPQTDARAITWHWELDPDFPAVRLDRAQMEQVF
                     LNVAKNAMESIDRNGRIIVRTRRDPQRDIIEIEDTGGGIAPDVHDRLFSPFFTTKPGG
                     NGIGLTLIREILASHGFEGSLENGVRGGALFRVRVGGS"
     gene            complement(9889..11256)
                     /locus_tag="VF720_03650"
     CDS             complement(9889..11256)
                     /locus_tag="VF720_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019947354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7878476.1"
                     /translation="MILIVDDDAAVVASLALLLKQRGHATVAASSPAEALALVDDREI
                     SLVLSDMNFSRETTGEEGLALLADIRHRRPTLPVLLITAWGSINLAVQGMKGGASDFI
                     TKPWSNEQVIQAVETAMGLAASGTAPSEETSRADLDLEYDLAGVVGEDPKFCRALQLL
                     MRVARTNAGILITGETGTGKEVVAEAVHRNSPRAGRSFVKVNLGGMPRSLFESEMFGH
                     VRGAFTDAHADRPGRFEAARGGSILLDEIGDLDLSCQVKLLRVLQDRSYEVLGTSVPR
                     PLDVRVLCATNVDLQQAIAAGTFREDLYYRINLIEARLPSLNERAGDIPLLARHFATQ
                     AAERYSLGEVAVDADALEWLKTRAWPGNLRQLDHLVERTLLTSGTRRLSTETLRRTLQ
                     AESGEDMRDQLPPVGSMTMDEIERAMILKSLKHHDGNITRAATALGMSRPALYRRLDK
                     HGIEA"
     gene            complement(11259..12491)
                     /locus_tag="VF720_03655"
     CDS             complement(11259..12491)
                     /locus_tag="VF720_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878477.1"
                     /translation="MIGHIVRMVWNRKRTTGLLLVELLVSYLVLCVVFSAAASYYINW
                     KKPLGYDYHDVLTLTASAPPTDDFDREARGHRLEEAARLEQAIRTLPNVVAVSPLVNV
                     PYSGSGSRRGLNMKDGSLIQFFISQASIDMKEVFKFSVVQGRWLEPADFQTAWTPIVV
                     NRALARRLFGNEDPIGRTIESYDENGTLRVAEDESDNMKVVGVIDDFRKGGEFSDSPY
                     AGFVPQYTDPGRAWPSDEYMVRVNPGTPAAFEEQLITFVRSVLPDYSVRVESLEASRA
                     TYFKTTLLPLIVGGIVAWFLILMVGMGLVGVLWQNVAQRSREFGLRRALGANGGDVVR
                     QVVGEMLALTTLAVLAGAVLFLQVPMLKLPGFINLRTALTAILAAVVVLYPFVALCSL
                     YPSWLAARVQPVEALHAD"
BASE COUNT         2125 a         4210 c         4225 g         2058 t
ORIGIN      
        1 ttgtgggacg gcatggcgag gcaccgatct acctggccga cgtggcgctg gtggaagacg
       61 gcttcgagga cgtgcgacgc gcggcccgcg tgaacggcga gccggcccag gggctcggca
      121 tccgcaagca gcggggcgcc aacgcggtgg cggtgggcga ggccgtcaag cacgagatgg
      181 aggagatccg gaagacgctt cccgagggga tggagctcgg catcaacttc gactccaccc
      241 agttcatcga ggagtcggtg cacgagatcc agttcgaact gatcctggcg gtgatcctga
      301 cggcgctcgt ctgctggatt ttcctgggat cattatcgag cacgctcaac gtgatcctgg
      361 ccatcccgat gtcgctgttc ggcacggtgg ccgtcatcta cttcctcggt tacaccttca
      421 acacgttcac gctgctcggg atggcgctgg ccgtcggcat cgtggtggac gacgccatca
      481 tggtgctcga gaacatccac cggcacggcg agatggggaa ggacaaggtg accgccgccc
      541 gcgagggaac ggccgagatc accttcgcgg ccctggccgc cacgctggct gtcgtggcca
      601 tcttcatgcc ggtcatcttc atcgagggcg tggtcgggcg cttcttcttc cagttcggcg
      661 tcacgctctg cgtggcggtg ctcttttcgt atctcgaggc gatcacgctg gcgccggccc
      721 gctgcgcgca gatgctcgac gtgtcacggg agaagcgggg caagatcggg cgcgcggtgg
      781 acgatgcctt ccacaagctc gaaaacggct accgccggct gttgccgctg gcgctgaacc
      841 ggccggtgat ggtgctcggc ggcgccacgc tcctctttgc cggcgccatc tggattctcc
      901 gctcgctccc ctcggaattc gtgccgagcc aggaccagag ccggctgatg gtgcgcgttc
      961 agacggccgt gggcgccgac cttcatgaaa cggatgcctt gttcgagaag gccgaggcca
     1021 aggtgtcggc ccatcccgaa gtgacgcgca tctttgcggt ggtgggcggc ttcggcggat
     1081 cgggcgtgaa ctccggcgtc atcttcgtga cgctggtgcc gcccaagcag cggaagatcg
     1141 ggcatctcga tttcgccgcg cagctgcgcc gcgaattgaa cggcatcccc ggcgtgcgcg
     1201 ccgtggtgca ggacccctcc cagcaggggc tcaccgcccg ccgcggcttc ccggtggagt
     1261 tctcggtgcg cggcgccaac tgggatgggc tggtggacgt gtcgacgggc ctcatgcgcg
     1321 acatggacgc ctccggcacc gtcgtcgacc tcgattcgga ctaccgcgtc ggcatgccgg
     1381 aactccgcat cgtgcccgac cgcgcccgat gcgccgatct cggcatcccg gtggaggaag
     1441 tggccgcggc catcaatgcg ctggtcggcg gcattcccgt cggcaagtac tccgaaggcg
     1501 ggcgccgcat ggatgtgcgc atgcgcctcc tggccgacca gcgcgcccgc cccgaagaca
     1561 tcgtgcgcct gcgcgtccgc accgccaacg gcgatctcgt gccgctctcc tcggtggtaa
     1621 cgaccgaaga gcgcccggcg ctccaggcca tcacccggcg tgaccgcgaa cgggccatca
     1681 cgctcacggc caacgtggcg cccggccact cgcagcagga cgcgctcaag gcggtggaag
     1741 gcctggcggc cgatgttccc gacggctatc gcgtggtgct gggcggcgcc tcggtggcct
     1801 tcaaggagtc gatggggagc ctgctgtttg cgctggtgat ggggatcatc gtggcgtaca
     1861 tggtgctggc ctcgcagttc aactcgctgc tgcaccccat cacggtgctc acgatcctgc
     1921 ccctctcggt ggccggcgcg gccggcgccc tcctcctgac cggcaagacg ctcaacatct
     1981 tcagcatgat cggcctgttg ctcctcctcg gcatcgccaa gaagaactcc atcatcctgg
     2041 tggactacgg ccgccagatc cgcgaccagg gaaagaacgc ccgcgacgcc atgctcgaag
     2101 cggggccgcg ccgcctgcgc cccatcctca tgaccagcgt ggccaccatg atggccgccg
     2161 tcccacccgc actcggcctc ggccccggct cggagatccg cacgccgatg gccatcggca
     2221 tcatcggcgg catcgtggtc tcgacggccc tctccctctt cgtggtgccg acgtttttcg
     2281 tgacggcgga caaggtgacg gaggggatca agggatcgat ggcgcggcgg aaggcgcggc
     2341 gcgagggacg tacggaagag gcggcgacgc aggcgtaggg gccagcgacg gcccttagcg
     2401 aatcacccat cgggggctca gacttgatct tccacggatc gcccagacga catgaccttg
     2461 gttccggacg gtgtctggac gtgtgcctgc agtcccgatt tccccggccg caccaccaat
     2521 ggacactcca ggccgattcg atccagaaac gcgcgcagcg cgaccacatc cggatgaaag
     2581 aggtcgagcc gctccaatga gcaccccgac cccgtcatcg tcgtcgtcgg gtgccgcccc
     2641 tccggccatt cgatcaggat cggcagggcg cctccccagg ggggtcggcc atctacgggg
     2701 atcgtgaggc gccaggtcag gtcgccccgc gtcatcgttt cgatcgaacc gggcggcacg
     2761 ggcgccagcg ccaccacggc ctcgatgtca tcgacccgca ccacccatcc cgccagccgt
     2821 ggctccgcgg tcatcgcggc atcgtcgagc gcaaaccagc gagggcgatc gggtggggcg
     2881 gcttgcgggt cgatcgccag aacttcgagg taggcgtcat ctcccaggcg gaggagcgtg
     2941 ttgtgggttc ccatgtacgg atgttggcca cctgggccgg gcgtgacacc gagccgcttg
     3001 gtcacccaat cggtgccggc ggccagtgaa ccggccgtga tgaccaggtg atcgaagcgg
     3061 ctgtgcattg gccctgtacc ctcagcccgc taccgcaaga atgtgcgcgg acaccgacaa
     3121 atccgatgac gaaaaacaac agaagatgac ttcgtcaaga ttggattgcg ccggagcgga
     3181 gcggacggtg gccaccacga tcgctcaatc ttccgcgggc aactccggca cgttcagcgc
     3241 cgccgcgatc agctcgcgcg cacgctccgc gtcttcccgc atcacctgga tcacgatcgg
     3301 ccccgtaagg aaattgtggc cgccgagcgt gccggcgccg atcaggttct gcacgccgac
     3361 atgccgcacc atgtaaggca cctcggcgtc ggtgagcacc gattcgatga gcgattgctg
     3421 gaccgggtcg gctacctcga ggacggtgac ctcgtcgggc tcggatgaca tggaaggctc
     3481 ctccgggtgg tgcgggccct acgcgtcgct cggggcctgg ttgctgggcg gctccttcag
     3541 cccaccttga tcagtttacc gcggcgactg ctgaggaccg gtacccccga accatacaac
     3601 gtcaattcgg cccggacgac atctctttcg gtccaggcac gaaaccggcc gccggccacg
     3661 gttccctgga ccaggtcata ggtgagcgac gtcggggtcg attcgcgcgt cagctccccg
     3721 accaggcgct cgtcgacgat ttcaatgcgc gtggcgccct gccccgctcg cacgcggagc
     3781 tccgggccgg ccggttcatc gcgataggcc gagtcaggga tggcatctcc cggaattccg
     3841 gtgacgggag cggccagctg ttcatcgaca agcaaacgcc attcgccgtc ggcgagagtg
     3901 gcgcggagcg actccgggcg acgctcggtt gcacagccca ggatcgagag ggcaaccaag
     3961 aggccgaggg tccgcgtcaa catgccggcc agttcatttc cgcttgaagt actgcaccgc
     4021 ccgctcatgc ttttcggcgg cggcccgcgt cttgaacgtg ccgaggttgc ggcggttgcc
     4081 ggtcttcgga tccttcctgc gcgagtagag gcggtacaca cccgacggga gctttcggat
     4141 catggtgcgt tactcctgta ttccggggcc gggacgattc cggtccacgt acacccacgc
     4201 gcgcatgccc gatgcgagcg tcaccgccac gcgcaggcac gcctccacct cgtaccgatc
     4261 ggcggcggcc agttcgtcgt cggtgatctc gaagacggtg ccggggatgc ggctcgtctc
     4321 ctggccgtcg tatgccgcaa tggtgtggac ggccttgccg ctgacggcca ccacgtgcgg
     4381 atcgtcgatg gcaagcggcg ccagaatgaa gccgatcatc gtatcgggcg ttccggagag
     4441 aagcctgcca aacgtcgcga tctggacgtt ttcctgctgc agcgtgccgt acgaaaagag
     4501 tcctggcatg gcttcagcgt actccgattc gggcgctcag gcatccgtgg acggcggccg
     4561 cagcacggca tccacgaagc gcgccacgct ctcgggcgca tggtcgaagt agagaaacgg
     4621 ttcgatcagt tcgacttcca tgatgaccat gtcggcgccg atgcgcacga ggtccagccg
     4681 ggcgtagaga tgccggaagt cgatgcgggc catgatggct ccggcttcgg ccaggtggtc
     4741 ggggcgcggc gtggcgactt cgtcggtgcc gccgtaagtc gtgttgatgc ggtagtcgcc
     4801 ggcggcgggg cgcttcctca cgagatggct gaagcggccg tcgatgaaga tgaacgacca
     4861 ctcgccctct tcccggatgg tttcgatcag gggctgcacg atgaagtcct tcgccacacc
     4921 cgccgcgcga agctcctgga gttcacttcg cagttcgccg agactcaccc gacgaacgcc
     4981 gaagcccgcc gcgccgacgc ggggcttgat cacggcatcg tcccacccgt gctcggccag
     5041 caacctgtga agatcaaagg ggtgagagag cccggctcga tacgtcggca ccgtcgggat
     5101 tccctgcgcg tacagttccg tgaggtagac cttgtcgctg ttccagcgca ccgtatccaa
     5161 cggattgatc aaccggcacg atgacgcttc gatgcgtttg agcaccgcca ggaattcgtc
     5221 gcgtgcgtcg atgtagtccc aggtgctccg gatgatggcg atgtcgtacg aattccagtc
     5281 aacgtcggga tcgccccacg acacggcctc ggccgacacg ccgaagcgat cgaattcggc
     5341 gatcagccgg cggtcatcgg cgaagaactc gtcgacattc gcaacctccc gggtgatgaa
     5401 ccgggggagc gcccgcaagg tcaggatggc gatgcgcacg caatcgtcct acggaccgat
     5461 caatcccatg ctggtgccgg aggcgtcggt gaagtagccg aaggtggcca tgcccggcat
     5521 ggccatcgga tcgcccacgc gctttccgcc ggcggcttcg atggcggcga tcgtggcgtg
     5581 gacatcggac acgtagatgt acgccaccgc ggggagcgcc ggcgtgtgcg actggaacac
     5641 gccgctgatc ccggcgcccg ggttgaacga catgtactgc ggcatcatgg ccgccgagcc
     5701 ccagccgaac tgctcgccga agaaggccgc ggcggccgcg ccgtcggcgg cgtacatctc
     5761 gaggctgcag atggagccgg gcaacggctt gggattgtcg ccgaagggca ccgggatggc
     5821 aggtgtcggg cgacccgcca tgccattcgt gacaccgtag atcgtaccgg acgcgtcctt
     5881 gaagcgcgca agcgtgccga ccggcgccac attccacggg gcccgctcga cggtggcgcc
     5941 tgcggcaacg acacgcgcca tcgccgcctc gacatccgat acgcgcaggt acggcacgat
     6001 ccccggaaac cccgcgggca ccctggtgcg gagcgcggcg gcggggccgt ccggcgtgat
     6061 gatggccgtc aattcgcccg agagtggctg catcttccaa ccgaacaacg aggaatagaa
     6121 cccggtcgac tcatccagtc gattggccga gatcacgacc agcgtgacgg ggtgctgtcc
     6181 gcttccggcg ggcatggtgg gcatttcgga cgtcatggga gatcctctcg agggagcaac
     6241 gggacattca aaccgccagg agcgacagag atcgagcagt gccacattac gctgtcgtga
     6301 gtcacggctt caccgcccgc tcgaggagct cgatcgcccc cgccgaggga acgcggaata
     6361 ccatcgacgg ccattccggc accaggcgat cgacaagcag aaacagcgcc agtccctcgt
     6421 cctgcgacca ccagcggcgc cggcccagca tcttcgatac cgcttcgtcg cccgtgaggc
     6481 cgccaccctg cggatgcgcc agccaggcct gcccggtcca ttgccccgct ccttcgagcg
     6541 agaggaaggt gtcgtccagg gcggcgaaga cggaatcggc atcgacgaac catctggcgt
     6601 gacgggcccg catcattccc agtgccttcg cggccagcgc gcgcgcctcg tcgtccgttc
     6661 cggtcctggc gccttcgtac aggagatcgc gctccgcgag atactccgcc acgtacgtgg
     6721 agtccctgcc gaagcggagc tgcaccacgt cgtcgctgag cggctccgga aacgtccagg
     6781 ccgaatcgag cgggcccatg acggcggcca ttccgggaat ctggcgtgtg tgggcgaact
     6841 catgcaggaa cacggcggtg agcccgggct cctggccgtg acccgcgctc ttccagaacg
     6901 ttggcgatcc catcacgaag aagacaccgc catgctcctt gctgccgccg gcaaaggtca
     6961 tgagctgtac ggccaggcga gtggaatcgg ggagccggat cgagtcgtcg tgcgcgacgg
     7021 cgcgccacgg gagctccgac aactggaacc tcggccccgc gatcggcgcg gcgccggcgg
     7081 ccgagacggc cgacgtggtg tatacgcaca ggctgtcgta gaagacaaag tacggcggcg
     7141 cctcggtcgg aatgtgcagg atttcgcgcg acgtcaggtc ccacgcattg aaccagcgtt
     7201 cgacccacgg cgtggcggag gtgagatcgc aggcgggaac ggctgttccc gggtccgagg
     7261 cgcgcgcggt gcgcgtgcct gccgccagga gggcggcagc tgccacaatc accagcgaaa
     7321 ggcgtctcac cacctggtca gcgccgctcc atgtaatgct cggtgaaccg cgtgcaacgg
     7381 gcctcttcgt cgaactccat cacgaagatg ttggcgaact cgcgcagcag cacgccatcg
     7441 cgcgtgtagc gagtgcgtcc ccgtgccacg ccgacatttc cctccacggc caccggctcg
     7501 tagtgcgcct cccacgaccc ggggtcatcg cgctccgaca gccagttcgc cacgatcgcg
     7561 tcgcggccgc gaacgggtgg ctccgtaccg aacggattga agtggtactc ggcgccggtg
     7621 ctgaacaggg ccccgatgcg ggaggaatcg taggtgcgcc aggcaccgat gtagtcatcg
     7681 agccattgct ggagttgcgg gcgggtcatg ggggcgttcc tcgctcggtc gatccggtgg
     7741 ttttcagcgc ggccgacgat cagctccttc cgagcgtacc atcggaccga tcacggcggc
     7801 gcacggtggt tcagccgcgc ctcgtgatca tgacgtgcgt ggcgcgctcg ccgggaatcg
     7861 cttccgtgca ggcgtaaccg agcgcatcca ggtcgaggcc gtggaagaga tgttcgcccc
     7921 ggcccagcag caccggaccg acggccaggt ggaggtcgtc gaccaggcgc gcctcgaggt
     7981 actggcggac ggtggccacg ccgccgccga tgcggatgtc ccggccgccc gccgcgtcac
     8041 gggcccggtc gagtgcctcg tggatgccgc cggtcacgaa gtgaaacgtg gtgccccctt
     8101 ccatcgtgag gggcgcccgc gggtgatgcg tgaggacgaa gaccggcacg tggtacggag
     8161 ggttcgggcc ccaccagccg cgccagctct cgtccggcca ggggccgcgg accggcccga
     8221 acatgttgcg ccccatgatc caggcgccca tctccatgct gtcctcggcg aagcggttgt
     8281 cgacacccgt ctcgccgcca tcctgtttct gcatcacggc aaacatccgg gtgggaaaga
     8341 gccaggccat gatctcgggg ccgcgaacgc cgagcgggtt ctcgagcgac tggtccgggc
     8401 cggcgctgaa gccgtcgagc gacatggaat aggcgaggac gcggagtctg gacatgggga
     8461 gactttagcg cagggggtgg ggcggcgacc aacagagggc caccgaccaa ccgtgggcca
     8521 ccaactctgg tgcagccaca gtggggtgat tgcgatacag gttcctcaac tacccccgac
     8581 tcgcacccgg aacagggcac ctccccggac accattctcc agcgacccct caaacccatg
     8641 gcttgccagg atttcccgga tgagcgtcag tccgatcccg ttcccgcccg gcttggtcgt
     8701 gaaaaaggga gaaaagagcc ggtcgtgcac atccggcgca atcccgccgc cggtatcctc
     8761 gatttcgatg atgtcgcgct gcgggtcgcg gcgcgtccgc acgatgattc gaccgttccg
     8821 atcgatcgat tccatggcgt tcttggcgac attcaggaac acctgctcca tctgggcgcg
     8881 atcgagccgg acggctggaa aatcaggatc gagttcccag tgccaggtga tggcgcgtgc
     8941 gtcggtctgc ggcttcagca cgcgggccgt ctcggtgagc atctcgttca cgttgcacga
     9001 ttccgtgtgc gggggcggca gccggatgac atcggcgaac cgtctcagga acgcattcat
     9061 gtgactggtc cggttgatgg ccaccgtgag cgactcgcgc cagtggtcgt cggcagggga
     9121 gtcgaggcag ctctggagca gggagatgac gctggcgctc gtgttgttga tctcgtgact
     9181 catcgtgcgg atgagctttt cgtaggccgc cttttcgctt tcatagagct cgcgcgtcat
     9241 ctcgtcgatc aggatgaacg accgcgcaaa gccgcggtcg atgtagggga gcttgaggca
     9301 gcgcaatcga cgccgtcccg ccagcgtcac caatcgcgat tcaccatcga ccagctgggc
     9361 cagttccgcc ccgagcgggt tcgaccaggc ggcggggggc gtgccgagga gcgagtcgcc
     9421 accccccagc agcgccgccg ccgccgggtt catctccacc gcgttcccgt cgaagtcgag
     9481 cgagatcatg ccgctcggcg aaagctgaac caaccgcttc agcagcgagt cctgctcgcg
     9541 attcaggatt cgttcgcggc ggagccgctc cgtcatggcg ttgtagacgc gcatgagccg
     9601 ttccacgtca tgcggccctt cctcgatgaa gcgcgtggtg aaatcgcccg attcaagctg
     9661 gttcagggcc tgctgcgtga gctcgtgcgg gcgcgtgagg tggcgaacga ggcggatgcc
     9721 ggccaggagc gaggccagca ggaaagcctc gaggccgagc agccagggac gcttcggcag
     9781 cgcgagcgtc gcgatggccg cgccgaagac gagatggacg aggacgaggt agagcaggaa
     9841 gcggttcctg agactcacgc gatcaccgac cccacgcggg cgcccgcctc acgcctcgat
     9901 accgtgcttg tccaatcgcc ggtagagggc ggggcggctc atcccgagcg ccgtggcggc
     9961 ccgggtgatg ttgccgtcgt gatgcttcag gctcttcagg atcatggccc gttcgatttc
    10021 gtccatcgtc atgcttccca ccggcggcaa ctggtcgcgc atgtcctcgc ccgactccgc
    10081 ctggagcgtg cggcggagcg tctccgtcga aagccgccgc gtgccgctcg tgagtagcgt
    10141 ccgctccacc aggtgatcga gctggcggag atttccgggc caggcccgcg tcttgagcca
    10201 ctccagcgca tcggcgtcca cggccacttc cccgagactg taccgctcgg cggcctgcgt
    10261 ggcgaagtgg cgcgccagga gggggatgtc gccggcgcgc tcgttcaggg aggggagccg
    10321 cgcctcgatc aggttgatgc gatagtagag gtcctcgcgg aacgttcccg ccgcgatggc
    10381 ctgctgcagg tccacgttgg tggcgcagag cacgcgaaca tccagtggac ggggcacgct
    10441 cgtgccgagg acttcatacg agcgatcctg cagcacgcgc aggagcttca cctggcacga
    10501 gaggtcgagg tccccgattt cgtccaggag gatcgagcca cccctggccg cctcgaaccg
    10561 ccccggacga tccgcgtgcg cgtcggtgaa cgcgccgcgc acgtggccga acatctcgct
    10621 ctcgaacagg ctgcgcggca tgcctcccag gttcaccttc acgaacgacc ggcccgcccg
    10681 cggactgttg cgatgcaccg cctccgccac cacttccttg cccgttcccg tctcgccggt
    10741 gatcaggatt ccggcattgg tccgggccac gcgcatcaac aattgaagag cgcggcagaa
    10801 tttcgggtcc tcaccgacca cgccggccag gtcgtactcg aggtccaggt cggcgcgact
    10861 cgtttcctcg gatggcgccg tgcccgaagc ggcgagcccc atcgccgttt ccaccgcctg
    10921 gatcacctgc tcgttgctcc agggcttggt gatgaagtcc gacgcgcccc ccttcatccc
    10981 ctgcaccgcc aggttgatcg atccccaggc ggtgatgagg agaacgggaa gcgtgggccg
    11041 gcgatgccgg atgtcggcca ggagcgccaa tccctcttcg ccggtcgtct cgcgggagaa
    11101 gttcatgtcc gagagaacga gcgagatttc ccgatcatcg accagcgcca gcgcctcggc
    11161 cggcgacgat gcggccacgg tggcatggcc gcgctgcttc aggagaagcg ccagcgaggc
    11221 caccacggcc gcgtcgtcgt ccacgatcag gatcatcgtc agtcggcgtg gagcgcttcg
    11281 accggctgga cccgggccgc cagccagctc gggtagaggc tgcagagcgc cacgaagggg
    11341 tacagcacga ccacggccgc aaggatggcg gtgagcgcgg tccggaggtt gatgaaaccg
    11401 ggaagcttga gcatcggcac ctgcaggaag aggacggcgc cggccaggac ggccagggtc
    11461 gtcaacgcca gcatctcgcc gacgacctgc cggacgacat cgcccccgtt ggcgccgagc
    11521 gcccggcgga ggccgaattc acgcgatcgc tgggccacat tctgccacag aacccccacc
    11581 agccccatgc cgaccatcag gatgaggaac caggccacga tgcctcccac gatgagcgga
    11641 aggagcgtcg tcttgaaata ggtggcccgg ctggcctcga gcgattccac ccgcacgctg
    11701 tagtccggca gcaccgatcg cacgaaggtg atgagctgct cctcgaacgc cgccggcgtg
    11761 cccggattca cgcggaccat gtactcatcg ctcggccagg cacgcccggg gtcggtatac
    11821 tggggcacga agccggcgta gggcgagtcg gagaactcgc cacccttccg gaagtcgtcg
    11881 atgacgccga ccaccttcat gttgtccgac tcgtcttcgg ccacccgcag ggttccattc
    11941 tcgtcgtacg actcgatggt cctgccgatc gggtcctcgt tgccgaacag ccgccgcgcc
    12001 agcgcccggt tgacgacgat cggcgtccag gccgtctgga agtcggccgg ctcgagccag
    12061 cgtccctgca ccaccgagaa cttgaagact tccttcatgt cgatcgatgc ctgcgaaatg
    12121 aagaactgga tcagcgagcc atccttcatg ttgagcccgc gacggcttcc gcttcccgaa
    12181 taggggacat tgacgagggg cgaaacggcc accacgttcg gcagggtcct gatggcctgc
    12241 tcgagccggg ccgcttcctc gaggcggtgt ccccgggctt cccggtcgaa gtcgtcggtc
    12301 ggcggggcac tggccgtcag cgtgagcacg tcgtggtagt cgtagccgag cggcttcttc
    12361 cagttgatgt agtacgacgc ggccgcgctg aagaccacgc acagcacgag gtacgacacc
    12421 aggagctcga ccaggaggag gccggtcgtc cgcttgcggt tccacaccat gcggacaatg
    12481 tggccgatca tcgtgaacct ccgttcaggg cgcgcaccgg ttccatccgg gacatgcgcc
    12541 acgccgggta gacacccgac aggatgccga agaatgccga caacaggagc gccacgccga
    12601 agacccggaa attcacgt
//
LOCUS       DASVOD010000174         2103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_950195, whole genome shotgun sequence.
ACCESSION   DASVOD010000174 DASVOD010000000
VERSION     DASVOD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950195"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF720_03660"
     CDS             <1..283
                     /locus_tag="VF720_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878478.1"
                     /translation="LDRYYRSRERLLTQVRRGSGTGAGLRGKGPNVRDVALEILSKGK
                     RAMNIAALASLVIRRKGGRAGANFTQNLGAALYRDRRFKRTGRGLYSAR"
     gene            complement(320..1588)
                     /locus_tag="VF720_03665"
     CDS             complement(320..1588)
                     /locus_tag="VF720_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878479.1"
                     /translation="MDLPWFARALGVMAALALVIVVARLAMRVASTPVALAGAVVLAA
                     DAGLAVWSQSGLESAGFALALTLLATVVAAVASDPSPRLLLLAGLLAGALPLVRPEGF
                     ALGALAVAVCWRSAGGKRYGRLLLLVPALAVAAALTAWRLGEYGAWLPASAAAKLTFR
                     PDALAAGAGSLVHGFGRRLVVVAGAAWLVNRLRSRASAGAALAAWRDMTVGSTILIAG
                     MVLSSGGDWMGHDRLILPVVPLLVVTAVAAFPAPRFGGAALFLFAALAVIGPAWHADR
                     IPLHGHAARRLGEWLAGNSPDSTLLGVAAAGAVPYHSGLRTIDALGITDPAIARTPPV
                     PGAAWMPGHGHYDTEKFLAARPDIIVWEYGTSWSRQRMAEPSEGVPVRRGDYRRELLR
                     HPSFRAGWRPMPGVPAEVEGWFSVFRRVRD"
     gene            complement(1893..1979)
                     /locus_tag="VF720_03670"
     tRNA            complement(1893..1979)
                     /locus_tag="VF720_03670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Leu,seq:gag)
BASE COUNT          370 a          737 c          748 g          248 t
ORIGIN      
        1 cctcgaccgc tactatcgtt cgcgcgaacg actcctgacc caggtgcgac gcggcagcgg
       61 cacgggagcc gggcttcggg gcaagggacc gaatgtccgg gacgtggccc tcgagatcct
      121 gagcaagggc aagcgcgcga tgaacatcgc cgcgctggcg tccctggtga tacgccggaa
      181 agggggacgc gccggtgcca acttcacgca gaacctgggc gccgctctct accgcgaccg
      241 tcgcttcaag cggacgggcc gggggctcta ctcggcccgc tgaccgcgga tcgagccgcc
      301 tcctgaatca gggggcggct caatcccgca cccgacggaa taccgagaac catccttcca
      361 cttccgccgg cacacccggc atcggccgcc agccggcgcg gaacgacggg tggcggagca
      421 gttcgcgccg ataatcgccg cgccgcaccg gcactccctc cgagggctcg gccatcctct
      481 gccgcgacca gctcgttcca tattcccaca cgatgatgtc cggccgggcc gccaggaact
      541 tctccgtatc gtaatgaccg tggcccggca tccaggcggc gccgggaacc ggcggggtcc
      601 tcgcgatggc gggatccgtg atgccaagtg catcgatcgt gcgaaggccg gagtgatagg
      661 gaacggcgcc cgcagcggcg acgcccagca gcgtggaatc tggggagttc cccgccagcc
      721 actcaccgag gcgccgggcc gcgtgaccgt gaagcggaat ccggtcggca tgccaggccg
      781 gcccgatcac ggcgagcgca gcgaacagga agagcgccgc gccaccgaaa cgcggcgcgg
      841 gaaatgccgc gaccgcggtc acgacgagca atggcacgac cggaagaatg agtcggtcgt
      901 gtcccatcca gtcgccgccg gacgagagca ccatccccgc gatcaggatc gtggatccca
      961 cggtcatgtc tcgccatgcg gcgagcgccg ctcctgccga tgcccgggaa cgcaagcggt
     1021 tcaccagcca ggccgcgcct gccaccacca cgaggcggcg cccgaacccg tgaaccagtg
     1081 aaccggcgcc ggcggccagc gcgtcgggcc ggaaggtgag cttggccgcc gcgctcgccg
     1141 gaagccaggc cccgtattcg ccgagtctcc acgccgtgag ggcggccgcc accgccagcg
     1201 ctggaacgag gagaaggagc cgtccgtatc gtttgcctcc ggcggagcgc cagcagacgg
     1261 ccacggccag ggcgccgagg gcgaaaccct cgggacggac gagtggcagc gcaccggcca
     1321 gaaggcccgc gaggagaagg aggcggggcg agggatccga tgcgacggcc gcgaccaccg
     1381 tggccagcaa tgtcaacgcc agcgcgaagc cggcgctctc caggccggac tggctccaga
     1441 cggcgaggcc ggcatcggcc gcaagcacca cggcccccgc cagcgccacc ggcgtggacg
     1501 cgactcgcat cgcgagacgc gccaccacga tgacgagagc cagcgccgcc atgacaccga
     1561 gcgcacgcgc gaaccatggg agatccatgc cgaaccgtgc gccaccggag aggagcgcca
     1621 cccaggcggg actggaccac gattcgaatc gaacgccggg attgaagacg gttccgagcc
     1681 cggacgcggc attggccgcg acgcggcagg cgatcagcga atcgtcgatg acattccagc
     1741 gggacagctg tccgtgcgtg acgaagctgg cgaggaaggc ggccagggcg gccggaacga
     1801 tgatccggga gcgggacgac ggcgtcgcca ggcggacctc ccgcgcgggc aggggcgcgg
     1861 ccgtcgccgc gttcctctgc cggtatggat gatggtgcgg aagaggggat ttgaacccct
     1921 acaggattgc tcccgccagc ccctcaagct ggtgcgtctg ccagttccgc cacttccgca
     1981 gtgggtgtcg gccccgaggg ccgacttgaa tcagttgccg gtgccggtcg aggtgcccgt
     2041 cgctgtaccg gtggtggtgc caggggtcgt caccggagtg cccgggttcg tcgccggctg
     2101 act
//
LOCUS       DASVOD010000175         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_219644, whole genome shotgun sequence.
ACCESSION   DASVOD010000175 DASVOD010000000
VERSION     DASVOD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219644"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..>1599)
                     /locus_tag="VF720_03675"
     CDS             complement(46..>1599)
                     /locus_tag="VF720_03675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7878480.1"
                     /translation="PATIPAAVPAMRPAGLGLKAGLVLMAFTLVLAFGFQGTRSLWEP
                     DEGRYAECAREMLVTGDFLTPRLGFEPHFTKPPLTYWSIAAPVALFGRNPWAARLYLS
                     LAYLATVLLVAALGARLWNRATGLLAGVIYATSLLPFIGASIATPDTLLVLWEVLALY
                     AFWRGWTAEDDRDRFAWPVVTGLAFGLAFLTKGPPGLLFLPAMLGFRASPAGRRAGAA
                     PLLNVTGAFLFLVVGGAWYLAVILRNKGLLPYFLADEVIGRLAGKHHRNAQWYGALVV
                     YVPSLLLGSFPWTFLWPAMWRHLDPASRGRGPSLWRARPKALLLALLVGIPLVVFTVS
                     RSRLPLYVLPLFAPLALAAARAAALRFGASFEGLSARFDRTARHRWAPRLAAWTVALL
                     GLRVAAAYWPNPQDDLAALRRLALPKVEELVFINAPPRYGMAFYAGLDVEATGWHGEA
                     AGSREDVEDEIEEVRQGNSHVFFLSPGEEGRFRKVMDRAGVQARERIELGHLVAYVVT
                     PPDPSTPTP"
BASE COUNT          293 a          525 c          588 g          193 t
ORIGIN      
        1 gtgtcgcgcg gcgggtgtgg gtaggaatgt cggggtggga aggaattacg gggtgggagt
       61 cgacggatcc gggggggtca cgacgtacgc gacgaggtgg ccgagctcga tgcgctccct
      121 ggcctgcact cccgcccggt ccatcacctt gcggaatcga ccctcttccc caggggagag
      181 gaagaagaca tgactgttcc cctgccgcac ctcctcgatt tcatcctcca cgtcttcccg
      241 cgatccggcg gcctcgccat gccagccggt ggcttccacg tcgagcccgg cgtagaacgc
      301 catcccgtag cgcggcgggg cgttgatgaa gacgagctcc tcgaccttcg gcagcgccag
      361 ccggcggagc gccgccaggt cgtcctgcgg attcggccag tacgcggcgg ccacgcgcag
      421 gccgagcagc gcaacggtcc acgccgccag gcgcggtgcc cagcgatgcc gggcagtgcg
      481 atcaaagcgg gcggacaatc cctcgaacga cgcaccaaac cgcagcgccg cggcgcgcgc
      541 ggcggccagc gcgaggggtg cgaacagcgg cagcacatag agtggaaggc gagatcgcga
      601 cacggtgaat accacgagcg ggatccccac gaggagcgcg aggagaagcg ccttcggccg
      661 ggcacgccag agcgacggcc ctcttccgcg cgaggccgga tcgagatgcc gccacatcgc
      721 cggccagagg aacgtccacg gaaagctccc caggaggagg gacgggacgt acacgaccag
      781 cgcgccgtac cactgggcat tccgatgatg cttgccggcc aggcgtccga tgacctcgtc
      841 ggccaggaag tacggcagca atcccttgtt cctcaggatg accgccaggt accaggcgcc
      901 tcccacgacg aggaacagga acgcgccggt tacgttgagg aggggggccg caccggcccg
      961 ccggcctgcc ggtgaggccc ggaagccgag catcgccggg aggaagagga gccccggcgg
     1021 gcccttggtg aggaacgcca ggccgaatgc gagaccggtg acgaccggcc atgcgaatcg
     1081 atcgcgatcg tcttcagcgg tccacccccg ccagaaggca tagagggcca gcacctccca
     1141 caggacgagg agcgtgtcgg gtgtggcaat actggccccg atgaacggga ggaggctggt
     1201 ggcgtagatc acgccggcca ggagaccggt cgcgcggttc cacaggcgcg cgccgagtgc
     1261 cgcgacgagg agaacggtgg cgaggtacgc cagtgacagg tagaggcggg ccgcccaggg
     1321 atttcgtccg aagagcgcca ccggggccgc gatgctccag taggtcaagg ggggcttcgt
     1381 gaagtgcggc tcgaagccga gtcgtggcgt gaggaaatcg ccggtgacca gcatctcgcg
     1441 ggcacactcc gcgtagcgcc cctcgtcggg ctcccacagg gagcgcgttc cctggaagcc
     1501 gaatgccagg accagcgtga aggccatcag gaccaggccg gctttcaggc cgagcccggc
     1561 gggccgcatc gcgggaaccg ccgcggggat cgtcgcggg
//
LOCUS       DASVOD010000176         7202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_532456, whole genome shotgun sequence.
ACCESSION   DASVOD010000176 DASVOD010000000
VERSION     DASVOD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7202
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532456"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1268)
                     /locus_tag="VF720_03680"
     CDS             complement(66..1268)
                     /locus_tag="VF720_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX7878481.1"
                     /translation="MTDKKRNGGTATKAAPGKNGAGAKAARPATTYGTPLPGPVIEPP
                     KRELLPGGVALIRDDDGNVIGPWPDGAAPPKLSRELARGWTGEQLLAVHRFMRLSREI
                     ENKVINLYRQGKISGGVYLGRGNEATAVSALYCLEPTDPVTILHRDLGAHLVRGWTAR
                     EIFMQYFAKRDAPNFGKDTGLHLGSLERFSLGTVSPLGCMIPVTLGCAVGLKMQGKKA
                     VGLTFTGDGATSLGDFHEALNFAGVWKAPFILIVENNQFAYSTPTGRQFACARISDRA
                     AGYGMPGTTVDGTDVLKMLNAVKTAVDRARKGDGPTLIESVTMRMRGHSEHDDASYVP
                     KGMADAWASWDPIVRFEEFLMAEGHLTQKKLDELLAELAVLIDESAEWAMTQPNSLGP
                     EAAEGVYA"
     gene            complement(1799..2503)
                     /gene="lipB"
                     /locus_tag="VF720_03685"
     CDS             complement(1799..2503)
                     /gene="lipB"
                     /locus_tag="VF720_03685"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX7878482.1"
                     /translation="MHAEAFANKQVNPHRPARRIWAGHVGYDAGQEIQKRLVGERQAG
                     AVPDTLLLLEHPLTYTIGRSGGDNNLLVPEVEIEKLGGTVHRVDRGGDITFHGPGQLV
                     GYPIVDLNDHYRDVHRYLRDLEEVLIRVLARWKIRADRVAGLSGVWVGQDKVAAMGVH
                     VSRWVTSHGFALNVSTDLAHFGRIIPCGIADRGVTSMERLLKSKLTLMEVAEATAEEF
                     AVVFGVSWEPDKWVPV"
     gene            2578..4041
                     /locus_tag="VF720_03690"
     CDS             2578..4041
                     /locus_tag="VF720_03690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX7878483.1"
                     /translation="MTQTDLLILGGGITGAMTACLAARRGWSVTLLERGDLASGASGA
                     TSHMLHGGLRYLEHGRLGLVREALHERRFWLDRAPEFSRAVRFLVPLRRDGRVGTLKL
                     RAGLWLYDRLAGSQNIEPASGHSSADIGRLEPSIDIASLSGGGAYTDGVVDDAGLAIA
                     LAREARAHGALIRTYREPISARRLDDGTFELTTRDSLAEDATRASDVIHARVIVNATG
                     CWSDATRAFLAAQLGLPTPSPRLKPSRGVHLVYPALTRGHGILFFARSDGRVLFVVPF
                     AGRSLVGTTEMPVDSPPTLEQQRPTRGEMAYLRRELANVIPESPGRRPLAVFSGIRPL
                     MSNGKAVGRASREHHVSNEAGVISIAGGKYTTARVMANDVLEAAARQLGRSSDAGHDA
                     LPARVDRWQDGPDPVALADESINDLFARRLDDVMRRRSHLWLEDDRGLDRVGAVATAM
                     GIRLGWSLARRNDEVDAYRGAVAAEQELIARSGDSNT"
     gene            4038..5525
                     /gene="glpK"
                     /locus_tag="VF720_03695"
     CDS             4038..5525
                     /gene="glpK"
                     /locus_tag="VF720_03695"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF000756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX7878484.1"
                     /translation="MTSPLVMAIDQGTTGTTVLLIDHDGLVAARGYAEIPQHYPKPGW
                     VEHDGDEIWASILDAAGQALRVDDGLASRIATIGITNQRETVLLWDRKTGRPVGPAIV
                     WQDRRTTDRCEQLRKARHERMIRKTTGLVLDPYFSGTKIEWLFRHDKSWKRAAAKGDL
                     VIGNIDTWVLWKLTGGEVHATDPTNASRTMLYDINKLAWSPKLLDLFGVPAACLPEVR
                     RSSGDFGTTRGAHPIPDGIPIGGIAGDQQAALFGQGCVKPGQVKNTYGTGAFLMVSLG
                     NRPRESKGGLLTTVACGPKGEAAWALEGSVFIAGAAVQWLRDGLAFFEKSHEIEALAA
                     SVDDSGGVTVIPAFVGMGAPHWRADARGAIFGLTRGTTRAHVARAVLEAIAQQSADVV
                     DVMQQDTGRRIVSLRVDGGATANNLLMQYQADLLGTPIQRPRMLETTALGAGILAGMA
                     AGIWASPRDAEAARTLDRTFTPSRPASWRRQERARWHEAVGRLLR"
     gene            5671..6180
                     /locus_tag="VF720_03700"
     CDS             5671..6180
                     /locus_tag="VF720_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878485.1"
                     /translation="MTKTKVRPSETEAPPVVVWRLIAHHAEVDKALRWFVSSKLIAVG
                     WGRIGDLSQMSLESPNDIAKLVSSSYPEMENSGSGGTSLWRFLNVMKKGDLVILGPKR
                     RLVMRIDGDYEYSNVRSPISPGDYRHQRRATRVAQDPEMLWLRCGAKIAPGEGMRWTV
                     ARCGDLAVS"
     gene            6185..7126
                     /locus_tag="VF720_03705"
     CDS             6185..7126
                     /locus_tag="VF720_03705"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7878486.1"
                     /translation="MRVVRLLILLALSAIPTNVSATPDSTTVILLGTGMPRPNPERSG
                     PATAITVGDRQFLFDAGPAVMRQLAAAKLSFKEFEAVFITHLHSDHTLGYPDVLLTSW
                     VMGRKTPLRVYGPPGLRRMTDLILEAWSEDVDIRTHGLERDLPGGEKADVREIEPPTS
                     SDPAAPLAARVRVVYDSADVRVTAIPVPHGSWKYAYGYRIDTPDRSIVISGDTAYSPL
                     LLEMSRGVDLLIHETYPLAYLKPEPRPNGELWPEYMHTFHTSDVEVGKLAAAARPKMV
                     ILHHVVWMGGSEAELIQGVRKGGYDGPVLIGADLGRY"
BASE COUNT         1188 a         2475 c         2312 g         1227 t
ORIGIN      
        1 cttgtcgcgg atcatctcct cgcggaggcc gtcgcccagc gcttcgatgt aggttttcac
       61 tgccattacg cgtacacccc ctcggcggct tccggcccga gactgttcgg ttgagtcatg
      121 gcccattcgg ccgattcatc aatcagcacg gcgagctccg ccaggagctc gtcgagcttc
      181 ttctgcgtga gatgcccttc cgccatgagg aattcctcga agcggacgat cggatcccac
      241 gaggcccacg catccgccat ccccttcggc acgtacgacg cgtcgtcgtg ctccgagtgc
      301 ccgcgcatgc gcatcgtgac cgactcgatc agcgtcgggc catcgccctt ccgcgcgcga
      361 tcgaccgccg tcttcaccgc gttcagcatc ttgagtacgt cggtgccgtc cacggtcgtg
      421 cccggcatgc cgtagcccgc cgcgcgatcg gaaatgcgcg cgcaggcgaa ctgccgcccg
      481 gtgggcgtgg agtaggcgaa ctggttgttc tccacgatca ggatgaacgg cgccttccac
      541 acgccggcaa agttcagcgc ttcatggaag tcgcccaggc tggtggcgcc gtcgccggtg
      601 aaggtgaggc ccacggcctt cttcccctgc atcttgaggc ccaccgcgca gccgagcgtg
      661 accgggatca tgcagcccag tgggctcacc gttccgagcg agaagcgctc gagcgatccc
      721 aggtggagcc ccgtgtcctt gccgaaattc ggggcatcgc gcttggcgaa gtactgcatg
      781 aagatctcgc gcgccgtcca gccccgcacc aggtgcgcgc cgagatcacg gtgcaggatc
      841 gtgaccggat cggtcggctc gaggcagtag agcgccgaca cggccgtggc ctcgttgccg
      901 cgcccaaggt acacgccccc cgaaatcttc ccctggcgat agaggttgat gaccttgttc
      961 tcgatttcgc gggagagccg catgaagcga tggacggcca gcagctgctc gcccgtccac
     1021 cccctggcca gctcgcgcga gagcttcggc ggcgccgcgc catccggcca cgggccgatg
     1081 acgttgccat cgtcgtcgcg aatgagcgcc acgccgcctg gaaggagctc gcgcttgggc
     1141 ggctcgatga cggggccggg gaggggcgta ccgtacgtcg ttgccgggcg agcggccttg
     1201 gcgccggcgc cgttcttgcc aggcgcggct ttcgtggcgg ttccgccgtt tcgtttcttg
     1261 tcggtcatca agaactcctt caggacctgg tcgagcccag tttttttctc agcgaaggag
     1321 aaaggtcgag tatggcgaag cgttcggtgt tgtagagata gtcttctccg gattcgtcaa
     1381 caacacggat caatccgtga cctgccgcat ccgcgtctgg aagcacctcg tagaccttcc
     1441 gtacttccag cgatacttgg taactctgat tatcgagaca gagagcaaat ttgggccaca
     1501 ccgtccgacc agtcactggt cgttcccgag cagccttttc accctgagtt cccgtcttcc
     1561 gatcccgtgg cattcgtacc agtggagctc cgcccattgg acgcttccgt cctccaggcg
     1621 tatcaaggcc atttcttttc gcttgcgcca ccgccccgcc ccgtactttc gcttcaactg
     1681 ctgcagcttg ttgatcgttc tcccgacagc gattgtctcc attccgcgta tcggtcccag
     1741 caactcgaac cacaccctcc ctcatgcttt ctggacggga aggtttcctg cagttacgtc
     1801 acaccggcac ccacttgtcc ggctcccagc tcaccccgaa caccaccgca aactcttccg
     1861 cggtggcctc ggccacttcc atcagcgtca gtttcgattt cagcagtcgc tccatcgatg
     1921 tgaccccccg gtcggcaatc ccgcacggga tgatccggcc gaagtgcgca agatcggtcg
     1981 agacgttgag cgcaaagccg tggctcgtca cccatcgact tacgtggacg cccatggcgg
     2041 ccaccttgtc ctgccccacc cagacgcccg aaaggccggc cacgcggtcg gcgcggattt
     2101 tccagcgggc cagaacccgg atgagcactt cctccaggtc gcgcaggtaa cggtgcacat
     2161 cccggtaatg gtcgttcagg tccacgatcg ggtagccgac gagctgcccc ggtccgtgga
     2221 acgtgatgtc ccccccgcga tcgacccggt ggacggtgcc gcccagcttc tcgatctcga
     2281 cttcggggac gagcaggttg ttgtcgccgc cgctccgccc gatcgtatac gtgagcgggt
     2341 gttcgaggag caggagagtg tcgggaaccg cacccgcctg acgttcgccg acgagccgtt
     2401 tctggatttc ctgcccggcg tcgtatccca cgtgcccggc ccagatgcgg cgggcggggc
     2461 gatgcgggtt aacttgttta ttggcaaatg cttcggcgtg catccaagtc ctcgaagggg
     2521 gcaaaaagct ccctcattat aatcgattgc gccccaatgg ctatgatggg ccccgacatg
     2581 acccaaaccg acctgttgat cctcggcggc ggcatcacag gcgccatgac agcctgcctg
     2641 gcggcccgtc gcggctggtc ggtgaccctg ctcgagcggg gcgacctggc ctcgggtgcc
     2701 tcgggcgcca ccagccacat gctccacggc gggctccgtt acctcgagca cggccgcctc
     2761 ggtctcgtaa gagaggcgct ccacgagcgt cggttctggc ttgatcgggc cccggagttc
     2821 tcacgggccg tgcgattcct ggtgccgctc cggcgggatg gccgcgtggg cacgctcaag
     2881 ctgcgcgctg ggctctggct ctacgaccgt ctggccggtt cgcagaacat cgagcccgct
     2941 tcgggccatt cgagtgccga catcgggcga ttggaaccgt cgatcgatat cgccagcctc
     3001 tcgggcggcg gcgcctacac cgacggcgtg gtggacgacg ccggcctcgc catcgccctc
     3061 gcgcgggaag cgcgggcgca tggggcactc atccgcacgt atcgggagcc catctccgcc
     3121 cggcggctgg atgacggcac gttcgaactg acgacgcggg actcactggc cgaggacgcc
     3181 acgcgcgcct cggacgtcat ccacgcccgc gtcatcgtca acgccaccgg ctgctggtcc
     3241 gacgccacac gcgccttcct ggccgcgcag cttggcctgc cgacgccatc accacgcctc
     3301 aagccctccc gcggcgtaca cctcgtctac ccggcgctca cccgcggtca cggcatcctc
     3361 ttcttcgcgc ggtccgacgg tcgcgtcctc ttcgtcgtgc ctttcgcggg gcgaagcctg
     3421 gtcggcacga cggaaatgcc ggtcgattcg ccgccgaccc tggaacagca acggccgacc
     3481 cggggcgaga tggcctacct gcgccgcgag ctggcaaacg tcatcccgga gagtccgggc
     3541 cggaggccgt tggcggtctt cagcggcatc cgccccctga tgtcgaacgg caaggcggtc
     3601 ggtcgcgcgt cgcgcgagca ccacgtgtcg aacgaagcgg gagtcatctc gatcgcgggg
     3661 gggaagtaca cgacggcgcg ggtgatggcc aacgacgtgc tcgaagccgc cgcgcgtcag
     3721 ctgggacggt catcggatgc gggccacgac gcgcttcccg cacgcgtcga tcgctggcag
     3781 gatggccccg atcccgtggc gctggccgac gagtccatca acgacctctt cgcgcgacgg
     3841 ctggacgacg tcatgcgacg tcgctcgcac ctctggcttg aggacgaccg tggcctcgac
     3901 cgcgtcggcg ccgtggccac cgccatggga atccgtctcg gctggtcact ggcccgccgc
     3961 aatgacgaag tggacgcgta ccgcggggcg gtggcggccg aacaggaact catcgcccga
     4021 tccggagact cgaacacatg acatcgccac tcgtgatggc catcgaccag ggcaccaccg
     4081 gcacgaccgt ccttctgatc gatcacgacg ggctggtggc cgcgcgcggt tacgccgaga
     4141 tcccgcagca ctatccgaag cccggatggg tggagcatga cggcgacgaa atctgggcca
     4201 gcatcctcga cgcggccgga caggcgctgc gggtggatga cggcctggcg tcgcgcatcg
     4261 ccacgatcgg catcaccaac cagcgcgaaa cggtgctgct gtgggatcgc aagacaggtc
     4321 gccccgtggg gcccgccatc gtgtggcagg accggcgcac gacggatcgt tgcgagcagc
     4381 tgcgaaaggc acgccatgag cggatgatca ggaagacgac cggcctcgtg ctcgacccgt
     4441 atttctcggg caccaagatc gaatggctct tccgtcacga caagtcgtgg aagcgcgccg
     4501 ccgccaaggg cgatctcgtc atcggcaaca tcgacacatg ggtgctctgg aaactgaccg
     4561 gcggcgaggt tcacgccacc gaccccacca acgccagccg cacgatgctg tacgacatca
     4621 acaagctggc gtggagcccg aagcttctcg acctgttcgg cgttccggcg gcctgcctcc
     4681 cggaagtgcg tcgttcgagc ggtgacttcg gcacgacgcg cggcgcccat cccattcccg
     4741 atggcatccc gatcggcggc atcgcgggcg accagcaggc cgcgctgttc ggccagggat
     4801 gcgtgaagcc cggccaggtc aagaacacgt acggcaccgg cgcctttctc atggtgtcgc
     4861 tcggcaacag acctcgcgaa tcgaaggggg gcctcctcac gacggtggcc tgcggcccga
     4921 agggcgaagc cgcctgggcg ctcgagggga gcgtcttcat cgccggcgcc gccgtgcagt
     4981 ggctgcgcga cggactcgcg ttcttcgaga aatcgcacga gattgaagca ctcgccgcaa
     5041 gcgtggatga ctccggcggt gtcaccgtca tcccggcatt tgtcggcatg ggcgctccgc
     5101 actggcgcgc cgacgcccgc ggcgccatct tcggccttac gcgcggcacc acgcgcgccc
     5161 atgtggcccg cgccgtcctc gaagccattg cccagcaatc cgccgacgtg gtagacgtca
     5221 tgcagcagga caccggccgc cgcatcgtgt cgctccgcgt cgacggcggc gctaccgcca
     5281 acaacctcct catgcagtac caggccgatc tcctcggcac gccgatccag cgcccgcgca
     5341 tgctcgaaac gaccgcactg ggcgcgggaa tcctggccgg catggcggcg ggaatctggg
     5401 cctcgcccag ggacgccgag gcggcgcgaa cgctcgaccg cacgtttaca ccctcaagac
     5461 cggcgagctg gcgccgtcag gagcgcgcgc gatggcacga agccgtggga cggttgttgc
     5521 gatgacgaca agcaggctcc ggctcgccga atcgggcatc gaccttggcc ccggaagtcg
     5581 ggaccgacac ccgtgtcatg cggaatccag aaactttttc tcatgggaga taatcccact
     5641 ccgggcggcc cgcggggtta tcattggccc atgacaaaga cgaaggtacg accgagcgag
     5701 accgaggctc cacccgtcgt cgtctggcgg ctgattgccc atcacgcgga agtggacaag
     5761 gcactgaggt ggttcgtgtc gagcaagctg atcgccgtgg gttgggggcg gatcggcgat
     5821 ctgagccaga tgtcgctgga atcgcccaac gacatcgcga agctggtgag ctccagctac
     5881 ccggaaatgg aaaactcggg atccggcggc acgagcctgt ggcgattcct gaacgtcatg
     5941 aagaagggtg atctcgtcat cctcggcccg aagcggcgac tggtgatgcg gatcgacggc
     6001 gactacgaat actcgaacgt gcgctcgccg atatcgcccg gcgactatcg ccaccagcgt
     6061 cgcgccaccc gcgtcgcgca ggatcccgaa atgctctggc tgcgttgcgg cgcgaagatc
     6121 gcccccggcg aaggaatgcg ctggacggtg gcccgctgcg gagacctggc tgtttcgtag
     6181 gcaagtgcgc gtcgtccggc tgctgatcct gctcgcgttg tcggcgatcc cgaccaacgt
     6241 aagcgccaca cccgattcca ccaccgtcat cctcctcggc accggcatgc cgcgccccaa
     6301 cccggagcgg tcgggtccgg ccacggccat cacggtcggg gatcggcagt ttctcttcga
     6361 tgcggggccg gcggtcatgc ggcagcttgc ggcggcaaag ctgtcgttca aggaattcga
     6421 ggccgtcttc atcacgcacc tgcacagcga tcacacgctc ggttaccccg acgtactcct
     6481 gacgagctgg gtgatggggc ggaaaacacc gcttcgtgtg tacgggccgc cagggctccg
     6541 ccggatgacc gacctgatcc tcgaggcatg gagcgaggat gtcgacatcc gcacgcacgg
     6601 cctggagcgt gatcttccgg gcggcgagaa ggccgacgtc cgcgagatcg agccgccgac
     6661 gtcatcggac cccgcggcgc cgctggccgc gcgtgttcgc gtcgtgtacg acagcgccga
     6721 cgtgcgcgtg acggccatcc cggtgccgca cggcagctgg aaatacgcct acggctatcg
     6781 catcgacacg ccggaccgat cgatcgtcat ctccggcgat acggcctaca gcccgctcct
     6841 gctcgaaatg tcacgcggcg tggatctgct catccacgaa acctatcccc tcgcgtacct
     6901 gaagccggag ccgcgcccca acggggagct ctggccggag tacatgcata ctttccacac
     6961 gtcggacgtg gaggtgggga agctggccgc ggccgcccgg ccgaagatgg tcatcctgca
     7021 ccatgtcgtc tggatgggtg gttcggaggc ggagctcatc cagggtgtgc gcaagggcgg
     7081 ctacgacggt ccggttctga tcggcgcgga cctgggacgg tactaggacg ccttcggcga
     7141 tttgtccagg cgctcggcga tccacatctt gatgaccgat tggcgcgtca cgccgaggcg
     7201 ct
//
LOCUS       DASVOD010000177         5795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_688973, whole genome shotgun sequence.
ACCESSION   DASVOD010000177 DASVOD010000000
VERSION     DASVOD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688973"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VF720_03710"
     CDS             <1..438
                     /locus_tag="VF720_03710"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7878487.1"
                     /translation="AIGGTQRIATAASIELAGRTIRLYSLHIGTWAELTREQRRDQVE
                     AVIADANTTEDDVIIGGDLNDSSLTSSFEAAGFTWLTRDIGRTCKFWSLDHVFTRGMV
                     ADGSRGKIADNRGSSDHCPVWVSVAGPEAAMSPRTPVASRTRS"
     gene            complement(354..1397)
                     /locus_tag="VF720_03715"
     CDS             complement(354..1397)
                     /locus_tag="VF720_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX7878488.1"
                     /translation="MIRTTLGVGVASAVLLLALAAAEPSGVVTIERLDPGFDRLVPRG
                     TRAERVAGGFAWVEGPVWDAASGALLFSDVPNNVIHRWSEAGGVTTELKASGYSGPVP
                     FSGREPGSNGLAFDSRGRLLICRHGDRRVVRREIDGTLTVLADRYQGRRLNSPNDLIV
                     RPNGDIWFTDPVFGLPGLANDPGKELSFQGVYRLTPDGTLTAMVTDLEGPNGIGFSPD
                     GRTLYVSNSDPRHPGWMAYPVDEDGTVGAGRQFADAAPFMKPGGGACDGLEVDEEGNV
                     WAAGPGGVHVFAPDGRRFGRIVTGVKTGNLAFGGDGSVLYIAAGHDLVRLATGVRGLM
                     AASGPATDTQTGQ"
     gene            complement(1394..1861)
                     /locus_tag="VF720_03720"
     CDS             complement(1394..1861)
                     /locus_tag="VF720_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878489.1"
                     /translation="MTAPDNDLDLTLAHRRLSAGCFNRTWEWIRKPDRTPEETEEMIH
                     AAHASAWHWLQRPDNAPKRRSVGCWLLSRVYSLAERPDEARRYAHLAAGAAMGESPFY
                     RAYALEALARAELRAGNAAEGRRLAGEAAGLAAQVEKDEDRRMLLDDLAELES"
     gene            complement(1896..3143)
                     /locus_tag="VF720_03725"
     CDS             complement(1896..3143)
                     /locus_tag="VF720_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TCR/Tet family MFS transporter"
                     /protein_id="HEX7878490.1"
                     /translation="MSAPRPPATRPAATGRSAAVTFIFITVVIDILALGIIIPVLPRL
                     VEGFLGGDTARAARMYGLFGTLWALMQFVFSPILGSLSDRFGRRPVILLSCLGLGLDY
                     VFMALAPSVGWLLAGRIISGITAASIATAYAYIADVTPPEKRAASFGMIGAAWGLGFV
                     LGPAVGGLLGAVNPRFPFWAAAALAFLNVIYGLFVLPESLPPESRRPFDWRRANPVGS
                     LGLLGQHAGLLGLATVNLLYYIAHQVLPSVFVLYAGYRYGWDPRMVGFTLAVVGVCNV
                     IVQAVLVKRVVGRFGARTTLLVGLVCGAIGFASYGLAPTAALFFLGVPIFAMMGFYSP
                     AAQSIMSRRVSASEQGRLQGANASIMGLSGLVGPGLFTQVFAAFIGEQAGWHLPGAAF
                     LLASILMFIAVPVALAATRKPTT"
     gene            complement(3140..3976)
                     /locus_tag="VF720_03730"
     CDS             complement(3140..3976)
                     /locus_tag="VF720_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX7878491.1"
                     /translation="MGYPRGADCTARPPRRRCRVLARHRPGRFLRRPAGRILNDVILA
                     GAALLAGAVAAVSGFGIGSLLTPVLLAAYPTAEAVALLSIPHVVASAVRLLPLRKEID
                     WRAFRDFGMASAGGGLAGALVQSRLASPLMGVILGVLLLMAGCAELFGKRLPPPKSKS
                     GRLIGGLLSGFFGGLVGNQGGIRSAALAGYRLSPRALVATATAAAVLVDLARVPIYLS
                     ARPELFVRGLPHLAIATIGVLAGTFLGVPVLGRIPERRYHLVLGLLLVLLGAGLLYRS
                     LT"
     gene            complement(3862..4491)
                     /locus_tag="VF720_03735"
     CDS             complement(3862..4491)
                     /locus_tag="VF720_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX7878492.1"
                     /translation="MIPAVEAIQRLRAGNERFVLRQLTHEVLLGRAHSPEHADGQNPF
                     AIVLGCSDSRAPAELVFDQGLGDLFVIRVAGNIVAPSQVGSVEFAAARFNTRLVVVLG
                     HTKCGAVEATLEVMRGTATAESRNLQSIVDRIRPSMEALMQTDLRNDPVELMRQAVRA
                     NVRASVNQLKHGSDILEGLIARHGLLVVGAEYSLGTGRVDFFDGLPDES"
     gene            complement(4514..4978)
                     /locus_tag="VF720_03740"
     CDS             complement(4514..4978)
                     /locus_tag="VF720_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7878493.1"
                     /translation="MSIPLLFLALLLSMTPNADAPKTARPTNAELAAQVRAAETAFAK
                     TMADRDHAAFSAMLADEAIFFGSRSVARGRDAVANQWKGLFAEKEAPFSWGPEEVEVL
                     DSGSLGISSGPVFAPDGKRIGTYNSIWRLEEDGKWRIIFDKGCPPCDCGEKK"
     gene            complement(5025..5423)
                     /locus_tag="VF720_03745"
     CDS             complement(5025..5423)
                     /locus_tag="VF720_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878494.1"
                     /translation="MTTLRMRVASLLVLVLSLAGCTAPAKKEDSPHAQKSPGAVADFR
                     DRVWRVTESNGVEPGMTYIWKSDGTLAIASPHGTPAFGTWRRNGQRLIMVEEDIEYPV
                     DILESTADHFRIVINNPGTPTELTMVPDQP"
     gene            complement(5474..>5795)
                     /locus_tag="VF720_03750"
     CDS             complement(5474..>5795)
                     /locus_tag="VF720_03750"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7878495.1"
                     /translation="FVVTQPRMPCFKLGIRHGRPDILKRFLATGRSGFYLSVAEEGVI
                     EAGQPILHTTVSGESLTVADINALYTSESQNRDLLGKAAELGHLTPNWRDYFCKRLAQ
                     LNDS"
BASE COUNT         1033 a         1956 c         1978 g          828 t
ORIGIN      
        1 gccatcggcg gcacgcagcg gatcgccacc gcggcctcca tcgagctggc cggtcgcacg
       61 atccgcctct acagcctgca catcggcacg tgggccgagc tcacacgcga gcagcggcgc
      121 gaccaggtgg aagcggtcat cgccgacgcc aacaccacgg aggacgacgt catcatcggc
      181 ggcgacctga acgactcgag cctcaccagc tcgttcgaag ccgccggctt tacctggctc
      241 acccgggaca tcggacgcac ctgcaagttc tggagcctcg atcatgtctt cacgcggggg
      301 atggtggcgg acggttcacg cggcaagatc gcggacaacc gtggatcgag cgatcactgt
      361 cccgtctggg tgtccgtggc cggtccggaa gcggccatga gcccgcgtac cccggtggcc
      421 agccgcacga ggtcgtgacc ggcggcgatg tagagcacgg agccatctcc cccgaacgcc
      481 aggttgcccg tcttcactcc cgttacgatc cgcccgaacc gccgtccgtc cggtgcaaag
      541 acatgcacgc caccgggccc cgccgcccag acgttccctt cctcatcgac ctcgagaccg
      601 tcgcacgctc cccccccggg cttcatgaac ggcgcggcat cggcgaattg gcgccccgcc
      661 cccaccgtgc cgtcttcatc caccgggtag gccatccatc ccggatgacg tggatcggaa
      721 ttggacacgt acagtgtgcg gccgtcgggc gaaaagccga ttccgttggg cccttcgagg
      781 tcggtcacca tcgcggtgag cgtgccgtcg ggcgtgaggc ggtacacgcc ctgaaaggac
      841 agctccttcc ccggatcgtt ggcgagacca ggcaggccga acaccggatc ggtgaaccag
      901 atgtcaccgt tcggcctcac gatgaggtcg ttgggactgt tgaggcggcg cccctggtag
      961 cggtcggcga gaacggtgag cgtgccgtcg atttcccggc gaacgacccg gcgatcccca
     1021 tgccggcaga tgaggaggcg cccccggctg tcgaaggcca gtccgttgct tcccggctcc
     1081 cggccggaaa atggaaccgg gccggaataa ccgctggcct tgagctcggt ggtcacgccc
     1141 cccgcttccg accagcgatg gatgacgttg ttcggcacgt ccgaaaaaag gagcgcgccc
     1201 gaggcggcat cccacaccgg cccctcgacc catgcgaaac cgccggccac ccgctcggcc
     1261 ctcgtgccgc gcggcacgag gcgatcgaac cccggatcga gccgctcgat cgtgaccacg
     1321 ccggagggct cggctgccgc gagcgcaagc agcaacaccg cggaagcgac gcccacgccg
     1381 agtgtcgttc ggatcacgac tccagttcgg ccaggtcgtc gaggagcatc cgccggtctt
     1441 cgtccttctc cacctgcgcg gccaggcccg cggcctcgcc agccagtctt cggccctcgg
     1501 ccgcgttgcc ggcccgcagc tcggcccggg ccagcgcttc gagagcgtac gcgcgataaa
     1561 aaggcgattc ccccatggcc gcgccggccg ccaggtgcgc gtagcgacgg gcctcgtccg
     1621 gcctttcggc cagtgagtag acgcgcgaga ggagccagca ccccaccgag cgacgcttcg
     1681 gcgcattgtc cggccgctgc agccagtgcc aggcggaggc atgggcggcg tggatcattt
     1741 cctcggtttc ttccggcgtt cgatcgggct tccggatcca ctcccaggtg cggttgaagc
     1801 agccggcgga gagacggcgg tgagccagcg tgaggtcgag atcgttgtcg ggagcggtca
     1861 tgcgggtact cctcagcgtc gagggacgcg gcacatcagg tcgtgggctt gcgcgtggcg
     1921 gccagcgcca cgggcacggc gatgaacatg aggatgctgg ccaggaggaa ggccgcgccg
     1981 ggaagatgcc atcccgcctg ctcgccgatg aacgcggcga atacctgcgt gaagagcccg
     2041 gggccgacca ggcccgacaa ccccatgatg ctggcattgg ccccctggag ccgcccctgc
     2101 tcggaggccg acacccgccg gctcatgatg gactgtgccg ccgggctgta gaagcccatc
     2161 atcgcgaaga tcggcacacc aagaaagaag agcgcggcag tcggcgcgag gccgtacgag
     2221 gcaaatccga tggcaccgca cacgaggccc accagcagcg ttgtccgggc gccgaagcgc
     2281 cccaccaccc gcttcaccag cacggcctgg acgatgacgt tgcagacacc caccacggcc
     2341 aacgtaaacc ccaccatcct ggggtcccaa ccgtaccggt agccggcgta caaaacgaag
     2401 acgctcggca acacctggtg ggcgatgtaa tagaggagat tgacggtggc caggccgagc
     2461 agcccggcat gctgcccgag gaggccgagc gaaccgaccg ggttggcccg ccgccagtcg
     2521 aaggggcggc gcgattctgg cggcagcgat tcgggaagga cgaagagccc gtagatgaca
     2581 ttgaggaagg ccagcgccgc ggccgcccag aaggggaagc gcggattcac cgccccaagc
     2641 aggccgccga ctgcggggcc gaggacgaag cccagccccc atgccgcccc gatcatgccg
     2701 aagctggccg cgcgcttttc gggtggcgtc acgtcggcga tataggcgta ggccgtggcg
     2761 atgctcgccg ccgtgattcc cgagatgatg cgccccgcga ggagccatcc caccgacggg
     2821 gcaagcgcca tgaagacgta gtcgaggccg agcccgaggc acgacaggag gatgaccggt
     2881 cgtcggccga agcggtcgga caaggagccc aggatcggcg aaaagacgaa ctgcatgagg
     2941 gcccacaacg tcccgaagag tccgtacatc cgggcggccc tggccgtgtc gcccccgaga
     3001 aatccttcca cgagccgcgg caggaccggg atgatgatgc cgagcgccag gatatcgatg
     3061 acgacggtga tgaagatgaa ggtgacggcg gccgaccgcc ccgttgccgc cgggcgcgtc
     3121 gcgggcggac gaggggcact cacgtgaggc tccggtagag caggccggcg cccagcaaca
     3181 cgagaagcag ccccaggacg aggtgatacc gccgttcggg gatgcgaccc aggacaggaa
     3241 cgccgaggaa cgtgccggcc aggacaccga tcgtggcgat cgccaggtgc gggagacccc
     3301 gcacgaacag ctcgggccgc gcggacaggt agatcggcac ccgtgccagg tcgaccagca
     3361 cggccgcggc ggtggcagtg gcaaccagcg cgcgaggcga caaccggtaa ccggccagcg
     3421 ccgccgatcg gatccctccc tggttaccga cgagaccacc gaagaagccg gagaggagtc
     3481 cgccgatcag ccggcccgac ttcgacttcg gtggtggcag gcgtttgccg aagagctcgg
     3541 cgcaccccgc catcaggagc agcactccga ggatgacgcc catcagcgga ctggccagcc
     3601 gggattgcac aagagcaccg gctagccccc cgccggcgga ggccatgccg aagtcccgga
     3661 atgcccgcca atcgatctcc ttgcgaagcg gcagcagtcg cacggcgctg gccacgacgt
     3721 gtgggatcga caggagagcc acggcctcgg ccgtgggata cgcggcgagc agcacgggag
     3781 tgaggaggct gccgatgccg aagccggaaa cggccgccac ggcgccggcc aggagtgccg
     3841 cccccgcgag gatgacgtcg ttcaggattc gtccggcagg ccgtcgaaga aatcgacccg
     3901 gccggtgccg agcgagtact cggcacctac gacgaggagg ccgtgccgtg caatcagccc
     3961 ctcgaggata tccgatccgt gcttcagctg gttgaccgag gcacgcacgt tggcccggac
     4021 ggcctggcgc atcagctcga ccggatcgtt gcggaggtcg gtctgcatga gcgcctccat
     4081 cgacgggcgg atccggtcga cgatcgactg gaggttgcgc gattcggccg ttgccgtgcc
     4141 gcgcatcacc tcgagcgtgg cctcaaccgc gccgcacttc gtatggccga gaacgaccac
     4201 caggcgcgtg ttgaaccggg ccgccgcgaa ttccacgctc cccacctgcg aaggggccac
     4261 gatgttgccg gccacccgga tcacgaagag atcccccaga ccctggtcga agaccagctc
     4321 ggccggcgca cgcgagtcgg aacagccgag gacgatggcg aaggggttct ggccgtccgc
     4381 atgttcgggg ctgtgggcac gccccagcag cacctcatgc gtgagctggc gcaggacgaa
     4441 cctctcgttc ccggcccgca ggcgctggat ggcctcgacg gccgggatca ttgcgtgcct
     4501 gccggttgga tgatcatttc ttctctccgc agtcgcaggg aggacacccc ttgtcgaaga
     4561 tgatgcgcca cttgccgtct tcctcgaggc gccagatgga attgtacgtt ccgatccgct
     4621 tgccatccgg ggcgaacacc ggccccgagc tgatgccgag cgaccccgaa tcgagcacct
     4681 ccacctcctc ggggccccac gagaacgggg cttccttttc ggcgaacagc cccttccact
     4741 ggttggccac ggcatcgcgg ccgcgcgcca cgctcctgct gccgaagaag atggcttcat
     4801 cggccaacat ggcggaaaag gccgcatgat cgcgatcggc catcgtcttc gcgaaggcgg
     4861 tttcggcggc ccgcacctgg gcggccagct cggcgttggt cggcctggcg gtcttcggcg
     4921 cgtcggcgtt cggcgtcatc gagagaagga gagccaggaa gaggagtggt atcgacatcg
     4981 gttcctccgt gcggcgtgag gtccggcgat caggggccgg accgtcaagg ttggtcgggg
     5041 accatcgtca gttcggtggg cgtgccagga ttgttgatga cgatgcgaaa gtggtcggcg
     5101 gtcgactcga ggatgtcgac ggggtactcg atgtcctctt caaccatgat gaggcgctgg
     5161 ccgttcctcc gccaggtgcc gaaggccggc gtgccgtgcg gggaggcgat tgccagcgtt
     5221 ccgtccgact tccagatgta ggtcatcccg ggctcgacgc cgttcgattc ggtgacgcgc
     5281 cacacccggt cgcggaaatc cgcaacggcg ccgggggact tctgcgcgtg cggggaatcc
     5341 tccttcttcg ccggtgcggt gcatccggcg agggagagca ccaggaccag gagggaggcc
     5401 acgcgcatcc gcaacgtcgt catccgatga ctctatcggc gaccaatgga gatgtccata
     5461 cgccgggaag gcgtcatgag tcgttcagct gggccaggcg tttacagaag taatcgcgcc
     5521 agttcggcgt gagatggccg agttcagccg cctttcccaa cagatcccga ttttgcgatt
     5581 cggatgtgta aagggcgttg atgtcggcca ccgtgaggct ctctcccgaa accgtggtat
     5641 ggaggatcgg ctggcccgcc tcgatcaccc cctcctccgc caccgacagg tagaaaccgc
     5701 tccggccggt ggccaggaat cgcttcagga tgtcgggccg gccgtgccgg atgccgagct
     5761 tgaagcaggg catccgcggt tgcgtgacga cgaat
//
LOCUS       DASVOD010000178         6796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_481026, whole genome shotgun sequence.
ACCESSION   DASVOD010000178 DASVOD010000000
VERSION     DASVOD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6796
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481026"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..3244)
                     /locus_tag="VF720_03755"
     CDS             complement(95..3244)
                     /locus_tag="VF720_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878496.1"
                     /translation="MTAGRPDPADLLAEALALPEAERPAFLDRACGADTALRGEIDSL
                     LAAHPGAQRFFDAPLVTPPLAGSEDPWIDRTVGAWRLLRRLATGGMGAVYLGERTDRA
                     FEKQAAVKLVRSGLDSAEVLARFRRERQLLADLEHPHIARLLDGGSTDGGLPYLVMEY
                     VDGHRIDEYADANRLDLSARLALFLAVCDAVQFAHKNLVIHRDLKPANILVDASGTVK
                     LLDFGIAGVVADGASPDLTATTSRRLTPRYASPEQVTGGRMSTATDVYSLGVVLFELL
                     VGRSPYRDTSSPYALERAVLEESIGRPSDHVSSKAAEHRRTTPARLSATLRGDLDTIV
                     LNALAKDPERRYPTVEALADDLRRHRDGRPVRARADTFVYRMSRFARRNTGLVAGVLA
                     AFVTMIAALVLVTNAYRRADADHRKAEWLAYTSSLAAAESSIRTSAVGEATRQLEAAP
                     VARRGWEWHHLHGRLDRSWKEWHPHEGGITQLVEAPAGDYVSLSVDGWIRASAVHGPP
                     VRSPVAHFDDAVESAAFLPGGVTLIAGLNNGSVYRVAMATGDTTRLFTGGPWARVAVS
                     PDGRTLAAGDFHGVVRTWDLATDRPGPAWQADHALVIPTFRPHDGRLITVGSDGVIRT
                     WHADGRRAGPDVPAHARRIYSFAWDRDGRRLALGSMDMTASVWDADSWTRLGVFREHR
                     GTIASLDFLEFEDRVVSAGADGRLLLWDIVSGQVTHELRGHTSDVSAVIATGGDSRII
                     SGDWAGGMRDWREGADDVRTIAVHPNRYMVPRVSDVRFDSAGTAVVVATNLTDVVEFD
                     VGGRERGRWHVGGVLQAIRIDDHRVAATTTDGELLVLGPDGDEPLVRVVAHRAGDTLL
                     EVRGSVIATTGADSLIRTWSAHVLTSNFTIKAGFLARALTITPDGSVLAAAGEAGHLA
                     AWQLSNGLQLWQTTVDSLDILDGAWQPDGRALILASASGVTRCTSGKAQPLASQPAAC
                     LALSPDGRRVAIGTEGQLVRILDAADGRELLTLHGHTGRLSVMTWSADGVFLASGAGD
                     GTVRLWDGVPASAVASGRD"
     gene            complement(3241..3810)
                     /locus_tag="VF720_03760"
     CDS             complement(3241..3810)
                     /locus_tag="VF720_03760"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7878497.1"
                     /translation="MNPSTDVTQLLHQAARKEPGSVERLYTAIYDDLRAVAEVRMRQE
                     SGDHTLQATALVHEAYLRLVDQTRCRWQDRAHFLAVASQVMRRALVDHARRRDAQKRG
                     GDWQRVPFEEALTIGAPDSGRALLAVDDALTRLASEHPDKARLVEMRYFGGLSHEECA
                     DVLAISTKTVSRHLDYAQAWLYREMTGRG"
     gene            3974..4810
                     /locus_tag="VF720_03765"
     CDS             3974..4810
                     /locus_tag="VF720_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878498.1"
                     /translation="MSLAIVRRPAALSAALLLLGLGSARASSIEMPAGSRSWIVVASS
                     DCDDDGPNVNVDRGHCVGAVSQLLPAGDVGHGQFGPGFAIVADATIGPELFRGSVRST
                     GNFTNTFVDMSMIDTYTLHSKTLPEGTTVSVTVHFEFEATLTPIFNVLAFGGGAVSIR
                     IGDGFQTAPIVIPENTRVTGNLGPTASQQVFYGVPQPSGAPIPIHLSATNSFTAQIGV
                     PFDRSYFLGLRPASSEIDFHNTGTIGFDLPDGVTITSTGGFGAVVPVAPASWSAIKSR
                     VR"
     gene            4906..5478
                     /locus_tag="VF720_03770"
     CDS             4906..5478
                     /locus_tag="VF720_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878499.1"
                     /translation="MRRRSLLPAVSGLMLLVASGCGDDDVNRTGPPITTVEDHLDFRD
                     AEGQPIDFGPGAETFIWCGPWESDVEEPALHVFVGTVDPPRAWQLMVVLDDVDPGESV
                     TFPNEFVFDQPRNAHLFIVDPPNQGATDTEDSFGAITFTRIACGTDGAVEFTIDATIG
                     SEFGDGPIVTVTGSFRGPVGNSPDWYDGGN"
     gene            5551..6003
                     /locus_tag="VF720_03775"
     CDS             5551..6003
                     /locus_tag="VF720_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878500.1"
                     /translation="MRHRATQLIGAKQLAIAAALVVANVLSACGGDDPPPPVTVEPTL
                     ASIQAEIFTPTCATASCHSTGQLEAGLDLSAGKSYDSLINADCTTEEADREGLWRVKP
                     GDPSKSFLIEKLRGVPNSKGRRMPYDGPYLSDAEIDVIEQWIAEGANP"
     gene            6000..>6796
                     /locus_tag="VF720_03780"
     CDS             6000..>6796
                     /locus_tag="VF720_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878501.1"
                     /translation="MKPNFRAIATSLLAVGLVLSAGCGGDSTGPGQVSCTDPGSICTA
                     VGTGTPAFRGEGAGPLQAALFLPIDIHFDESNRLLILDLNNFRIRRVDSDNKLRTIMG
                     TGVEAPVVNGSPALETPLHHTFSIARDGLGGFYLAGNHEPRILYCSPDGHAYVTAGVT
                     LPGLGGEGVPATEASFDAPCGVAVGPGGSPIWVADTNNHRIRRIDADGIVTTIAGNGT
                     VGHSGDGGPAIDARFRLPYRVRYDASSGDLYVCDTGNHAVRRIDSAG"
BASE COUNT         1041 a         2329 c         2351 g         1075 t
ORIGIN      
        1 atgggtggcc cggcgccgtt ccacgcgaag aacaggcagg agttcgcgaa tgcgctggat
       61 cgggtgctga cgcggcggtt gcgttagtcg acggtcaatc ccgcccggaa gcgaccgcac
      121 tcgccggcac tccatcccac aaccgcaccg tcccatcgcc cgctccgctg gccaggaaca
      181 ccccgtcggc cgaccaggtc atcactgaga ggcgtccggt gtggccgtgg agcgtcagga
      241 gttcgcggcc gtcggcggcg tcgaggatgc ggacgagctg gccttccgtt ccgatcgcga
      301 cgcggcggcc gtccggactg agggcaaggc aggcggccgg ttgggaggca agaggctgcg
      361 cctttccgga tgtgcatcgt gtcactccgg aggccgacgc gaggatcagc gcgcggccgt
      421 cgggttgcca ggcgccgtcg aggatgtcga gggagtcgac tgtggtctgc cacagttgca
      481 atccgttcga tagctgccat gccgcgaggt ggccggcttc accggcggcg gccagcaccg
      541 aaccgtccgg cgtgatggtg agggcgcgcg cgaggaatcc cgccttgatc gtgaaattgg
      601 aagtcagcac gtgggcggac caggtacgaa tgagggagtc ggcgccggtt gtcgcgatca
      661 ccgagcctcg tacttccagc agggtgtcgc cggcacgatg cgcgacgacc cggacgagcg
      721 gttcatcgcc atccgggccg aggacgagga gttcgccgtc cgtcgtggtt gccgccacgc
      781 ggtgatcgtc gatgcgaatg gcctgcagca ctccgccgac gtgccagcga ccccgttccc
      841 ggccacccac atcgaattcg acgacatccg tcaggttcgt ggcgaccacg acggccgtgc
      901 cggcgctgtc gaaacgcacg tcgctcacgc ggggcaccat gtagcgattc ggatggacgg
      961 cgatcgtccg gacgtcgtcg gcgccttcac gccaatcgcg cattccaccg gcccagtcgc
     1021 cggaaatgat ccgtgagtca ccaccggtgg cgatgacggc ggagacatcc gaggtgtgcc
     1081 cgcgcaattc atgggtcacc tggccggaca cgatgtccca gaggagcagt cgcccgtcgg
     1141 cccccgcgga gacgacgcgg tcctcgaatt cgaggaagtc gaggctggcg atggtgcccc
     1201 gatgctcgcg gaaaacgccg agccgggtcc aggagtcggc atcccacacg gaggcggtca
     1261 tgtccatgct gcccagcgcc agtcgcctgc cgtcccgatc ccaggcgaac gagtagatgc
     1321 gtcgcgcgtg tgcgggcacg tcgggaccgg cgcgccggcc gtccgcgtgc caggtgcgaa
     1381 tgacgccgtc ggagccgacg gtgatcaacc gtccatcgtg cgggcggaac gtggggatga
     1441 ccagggcgtg gtcggcctgc cacgcgggtc cggggcggtc ggttgccagg tcccacgtac
     1501 ggaccactcc gtggaagtcg ccggccgcca gggtgcgtcc gtccggcgaa accgcgacac
     1561 gggcccaggg tccgccggtg aagagccgcg tcgtgtcgcc ggtcgccatc gccacacggt
     1621 agaccgaccc gttattgagg cccgcgatga gcgtgacgcc accggggagg aaggcggcgc
     1681 tctcgacggc atcatcgaaa tgcgcaaccg gcgaacggac cggaggcccg tgaacggccg
     1741 aagcgcggat ccagccgtcg acggagaggc tcacgtaatc accagccggt gcctcgacca
     1801 gctgcgtgat tccgccttcg tgcggatgcc actccttcca gctccgatcc agccgcccgt
     1861 gcaggtgatg ccactcccat ccacgccggg ccacgggggc cgcctcgagt tgccgcgtgg
     1921 cctcgccgac ggcgctcgtc cggatcgacg actccgccgc cgccagcgaa ctggtgtagg
     1981 ccagccactc cgccttgcga tgatcggcgt ccgcgcggcg gtaggcgttc gtcacgagca
     2041 ccagcgccgc gatcatcgtc acgaatgccg ccagcacgcc ggccaccagt ccggtgttgc
     2101 gtcgggcgaa gcgcgacatc cggtagacga aggtgtccgc gcgcgcccgg acgggtcggc
     2161 cgtcgcgatg gcgccggagg tcgtcggcca gggcctcgac ggtggggtaa cgtcgctcgg
     2221 ggtccttggc cagcgcgttg agaacgatcg tgtcgaggtc gccgcggagc gtggcggaca
     2281 ggcgggcggg cgtggtccgg cgatgctcgg ccgctttgga ggacacgtgg tcgctcgggc
     2341 ggccaatcga ctcctcgagg acggcgcgct cgagggcata gggggacgac gtgtcgcggt
     2401 agggcgaacg accgaccagc agctcgaaca gcacgacgcc caggctgtac acgtcggtgg
     2461 cggtggacat ccgcccgccg gtcacctgct cggggctggc gtagcggggt gtgaggcgtc
     2521 gtgaggtcgt ggcggtgagg tccggagacg cgccgtcggc caccacgccg gcgatgccga
     2581 agtcgaggag tttcacggtg ccggacgcat ccaccagaat gttggccggt ttcagatcgc
     2641 ggtggatgac caggttcttg tgggcgaact gcacggcgtc gcacacggcg aggaagagcg
     2701 cgaggcgcgc ggaaaggtcg aggcggttcg cgtcggcgta ttcgtcgatc cggtggccgt
     2761 ccacgtactc catgacgaga taggggagtc ctccgtcggt ggagccgccg tcgaggagcc
     2821 tcgcgatatg gggatgctcg agatcggcca ggagctgcct ctcgcgccgg aagcgggcca
     2881 ggacctcggc ggaatcgagc ccgcttcgca ccagtttcac ggcggcctgc ttctcgaagg
     2941 cgcggtcggt ccgctcgccc aggtacaccg cgcccatgcc gccggtggcc aggcgccgga
     3001 gaagtcgcca ggcgccgacg gtgcggtcga tccacgggtc ctcgctgccg gcgagcggtg
     3061 gggtcaccag cggcgcgtcg aagaagcgtt gtgcgccggg gtgggccgcc agcagggaat
     3121 cgatttcgcc gcgcagcgcg gtgtcggcgc cgcaggcgcg atcgaggaac gcggggcgtt
     3181 cggcttcggg aagcgccagc gcctcggcca ggaggtcggc gggatccggt cggccggccg
     3241 tcatccgcgc ccggtcatct cgcggtagag ccaggcctgc gcgtagtcga ggtggcgcga
     3301 caccgtcttg gtggagatgg ccagcacgtc ggcgcattcc tcgtgcgaga ggccgccgaa
     3361 gtagcgcatc tcgacgagac gggccttgtc ggggtgctcg ctggccaggc gcgtcagcgc
     3421 atcatccacg gccagcaggg cgcgcccgga atcgggcgcc ccgatcgtga gcgcttcctc
     3481 gaacggcacg cgctgccagt cgccgccgcg tttctgcgcg tcccgccggc gggcgtggtc
     3541 caccagcgcc cggcgcatca cctggctggc caccgccagg aaatgggcgc gatcctgcca
     3601 ccggcagcgc gtctggtcga ccagccggag gtaggcctcg tgcacgagcg cggtggcctg
     3661 gagagtgtga tcgcccgatt cctgtcgcat ccggacctcg gccaccgctc tcaagtcgtc
     3721 gtagatggcg gtgtacaggc gctccacgct gccgggctcc ttgcgggccg cctggtggag
     3781 gagctgggtg acgtcggtcg acgggttcat ggcgggtgtc ctaactccct tctggatcat
     3841 caattgtaac cgatggacca gccgccgatg ccgcgtgtcc ggtggcgccg ggaaattgcg
     3901 cgtaaccgtc ggcctgttgt ccgatcggcg gaccgacagc ctgccgcgga catccagcca
     3961 aggagcgtca cccatgtcac tcgcgatcgt tcgccgtccg gcggccctgt cggccgccct
     4021 cctccttctc ggactcgggt ccgctcgcgc ctcgtcgatc gagatgccgg ccggctcccg
     4081 gagctggata gtcgttgcct ccagcgactg cgacgatgac ggtcccaacg tcaatgtcga
     4141 ccgggggcac tgcgtcgggg cggtttcgca gctcctcccc gccggcgacg tcggtcacgg
     4201 acagttcggc ccgggcttcg ccatcgtggc ggatgcgacg atcggccccg agctctttcg
     4261 gggatcggtg aggtcgaccg gcaacttcac caacacgttc gtcgacatgt ccatgatcga
     4321 tacctacacg ttgcacagca agacgttgcc cgaaggcacc accgtttcgg taaccgtcca
     4381 cttcgaattc gaggccacgc tcacgccgat cttcaacgtg ctggccttcg ggggtggagc
     4441 cgtgagtatc cgcatcggcg acggtttcca gaccgccccc atcgtcatcc ccgagaacac
     4501 ccgcgtcacc ggaaatctcg ggccgacggc ttcccagcag gtgttctacg gagtaccaca
     4561 gccgagcggc gcaccgatcc cgatccatct cagcgccacc aactcgttca cggctcagat
     4621 cggcgtcccc ttcgatcgtt cctacttcct cggtctccgt ccggccagct ccgagatcga
     4681 ctttcataac accggcacca tcggcttcga cctgccggac ggcgtgacga tcacctcgac
     4741 cggcggtttc ggggccgtcg tgccggttgc gcccgcgagc tggagcgcga tcaagtcgcg
     4801 ggttcgataa gccaccggtc gccgcgatcg gcgctatgat gggggggcct cccgcgccgc
     4861 ggggggctcc tttcatcgtc caacggcgac atcgacggag gcctcatgcg acgccgctcc
     4921 ttgctccccg cagtatccgg cctgatgctc ctcgtggcct ccggttgcgg cgatgacgac
     4981 gtgaatcgga ccgggccgcc catcaccacc gtcgaggacc atctcgactt ccgcgatgcc
     5041 gaggggcagc cgatcgactt cggacccggc gctgagacgt tcatctggtg cggcccgtgg
     5101 gaaagcgatg tcgaggagcc ggcgctgcac gtcttcgtgg gaacggtgga cccgcccagg
     5161 gcctggcagc tgatggtggt gctcgacgac gtcgatccgg gtgagtctgt tacctttccc
     5221 aacgagttcg tcttcgacca gccgcgcaat gcccacctgt tcatcgttga cccgccgaac
     5281 cagggggcta ccgacaccga ggattcgttc ggggcgatca cgttcacccg gatcgcctgc
     5341 ggcacggacg gcgccgtcga gttcacgatc gacgccacca tcgggagcga gttcggcgac
     5401 ggtccgatcg ttaccgtgac ggggtccttc cgcggcccgg tgggtaactc cccggactgg
     5461 tacgacggcg gcaattgagc ccccggttgg gctatcattg gttccgtcac gcggggagtc
     5521 gccccgcctc agtccccgga ggaccggacc gtgcggcatc gcgcaactca actcattggt
     5581 gctaaacaac ttgccatcgc agcggccctg gtggtcgcca atgtgctttc cgcttgcggg
     5641 ggtgatgatc ctccgccccc ggtcaccgtc gagcccacgc tggccagcat ccaggccgaa
     5701 atcttcaccc ccacctgcgc caccgcgagc tgccacagca cgggccagct ggaagccggc
     5761 ctcgacctgt cggccggaaa gtcgtacgac agcctcatca atgccgactg caccaccgag
     5821 gaggccgatc gcgaggggtt gtggcgcgtg aagccgggcg atccctccaa gagcttcctc
     5881 atcgagaagc tgcgtggcgt gcccaattcg aaggggcgcc ggatgcccta cgacggcccg
     5941 tatctctccg acgccgagat cgacgtcatc gagcagtgga tcgccgaggg cgccaatcct
     6001 tgaagccgaa tttccgggcg atcgccactt cgctgctggc cgtcgggttg gtcctgtcgg
     6061 ccggctgcgg cggcgattcc accggccccg ggcaggtgtc gtgcaccgac ccggggagca
     6121 tctgcaccgc cgtgggcacg ggcacgccgg cgtttcgcgg cgagggtgcg gggccgctcc
     6181 aggcggccct cttcctgccg atcgacattc acttcgatga atcgaatcga ctcctcatcc
     6241 tcgacctgaa caacttccgg atccgccgcg tcgattcgga caacaagctg cgcacgatca
     6301 tgggcaccgg agtcgaggcg cccgtggtga acggcagccc ggccctcgag acgcccctgc
     6361 atcacacctt ctcgatcgcg cgcgacggcc tggggggctt ctacctggcc ggcaaccacg
     6421 agccgcgcat cctctactgc agtccggatg gtcacgccta cgtaacggcg ggtgtcaccc
     6481 tgccgggcct gggaggcgaa ggcgttcccg ccaccgaagc ctcgttcgac gccccctgcg
     6541 gcgtggccgt gggacctggc ggctcgccga tctgggtggc cgacacgaac aatcaccgga
     6601 tccgccgcat cgacgccgac ggcattgtga ccacgatcgc cggcaacggc accgtgggtc
     6661 attcgggcga cggcggcccc gccatcgacg cgcgcttccg cctcccgtac cgggttcgct
     6721 acgacgccag cagtggagat ctgtacgtgt gcgacaccgg caaccacgcc gtgcgccgca
     6781 tcgactcggc cggcat
//
LOCUS       DASVOD010000179         3083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_845842, whole genome shotgun sequence.
ACCESSION   DASVOD010000179 DASVOD010000000
VERSION     DASVOD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845842"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VF720_03785"
     CDS             complement(<1..533)
                     /locus_tag="VF720_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX7878502.1"
                     /translation="MTLSAAAHNDLQRLQELMRHQVPDGDPALIVARALALLREQVEK
                     RRLGLRRNSVRKRSHAVRLEAARVIGKNPCSRYIPAAIRHEVAARDLQQCAFVTADGR
                     RCGERNWLEFHHIVPHARGGRAIAGNLELRCRAHNRYEAEREFDGSVREGAGRYEASG
                     WNQAGRADFVDTGANRN"
     gene            complement(1573..2079)
                     /locus_tag="VF720_03790"
     CDS             complement(1573..2079)
                     /locus_tag="VF720_03790"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEX7878503.1"
                     /translation="MATTRKPEKGKAGKRKSVTRGGEKGNASRKKAAPGRTVSSRGGD
                     RASRASATSPGRASTAADRTRTTAADRSRITGNDIAAQVTEQRRRLDDLDRRIIDLVV
                     ERASVVRSVARVKRTVAMDIIDVARETEIVELLRERAAGRLPEDELDNFIDALRELMQ
                     GVAMEREH"
     gene            complement(2057..>3083)
                     /gene="scpB"
                     /locus_tag="VF720_03795"
     CDS             complement(2057..>3083)
                     /gene="scpB"
                     /locus_tag="VF720_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX7878504.1"
                     /translation="ARGATARARRNHPMERDDLEAGGLDDVGADDPGMTDEKGGEELV
                     AASADPVTANGEDAGDTSLLADREARKSLVEALLFASDEPLTFALLKKATGNMAAAEL
                     RVVLEELKVEYDASRRGVALLEVAGGYVLVTRESWAPWVERLMKGKRKVRLSRAALET
                     VAVVAYKQPITRGEIERIRGVDAGGVLATLLERDLVMIKGRDPGPGKPLLYGSTQEFL
                     NYFGLNKLSDLPRLDEISALAAKNPAWSDAEKARFERAGIEPIDFEGDQPPALELGAG
                     ADSDGGDGRRFGVGDDSEPETRDSGEHGLPEGLEAVDSRYFREPLEPAENDDDSTERH
                     RGDDAEA"
BASE COUNT          483 a         1046 c          986 g          568 t
ORIGIN      
        1 tgattgcggt tggctccggt gtcaacaaag tcggcccgac ccgcttgatt ccagccgctg
       61 gcttcgtatc ggccggctcc ctcccgcacg gacccatcga actcgcgctc cgcctcgtag
      121 cgattgtgtg cccgacagcg cagttccagg ttccccgcga tcgccctccc cccgcgcgca
      181 tgcggcacga tgtgatggaa ctcgagccag ttccgttcgc cgcatcgccg accatcggcg
      241 gtgacgaacg cgcattgctg caggtcgcgc gcggcgactt cgtgccggat ggcggccggg
      301 atgtaccggg agcatggatt ctttccgatc acgcgtgccg cttccagccg gaccgcgtgc
      361 gatcgctttc ggacggaatt gcgccgcagc cccagccgcc gcttctcgac ctgttcgcgc
      421 aacagcgcca gtgcccgggc cacgatgagc gccggatcgc cgtccggtac ctggtgtcgc
      481 atcagttcct ggagccgctg gaggtcgttg tgagccgcgg cgctcagcgt catgtgcagg
      541 cgccaggcgg tttcggggtg attggtcagt gagggtgagc tggccggact cggactcacc
      601 tccggcgatg acccgtttgt ggctgctgaa tcaggtgccg ccccgttcgg acaggaacct
      661 gtttccgaac gactcgaatt gaagaactcg agagtcacgg gggtctcagg ccgctcgcgc
      721 cgccgaggct cgggtaccgc caccgcctgc accaccagcc gtgtggagga aaccgacggg
      781 gccatgaatc gggccacgat caaatcaatt gcttcacggg atgcatgccg tgcctcatcg
      841 agaagcgctc gatgattttc gggggtcagg tgcggtgaca ggcgggcaat cgcactcaga
      901 tggatgtcat tcgaccgcaa ccgttccagc accaggggaa agcgacgccc cgcgcgcgcc
      961 gcccggatac gcgtgaaggc ggcggcttcg ctgagatgaa gtattccggt gcaccagcgg
     1021 aacagcgaag gagcgccgtc cgcgagatag gcctgacgca catcgagttc acctagatga
     1081 cacagcaggt caacgaggtg agcgcgttca agccgagcca ggttcttgac gtgcgacagg
     1141 agctcttcgt tcgtgagccc cgagggctcg agtgtcgtag gttgcatggc gagagcgtct
     1201 catgcggttg ctctcgagct gtacgcatcg cgcgtcgatc cgaacgcgat catcgagcga
     1261 ttttccaagc atcgggcccg tttgcacggg gaggtggcga atgaggtggt gcaggaggcg
     1321 ttgcagtaat gacagcagca acagcgaacc gtgctcgata ttttctgtca agaactttcg
     1381 ataaatcttt tcatgatcga atcacgatgt gatcatcatc gaacgattgc ggttcactcc
     1441 aactgtttcc ggaaacagtt gactgtattc aatacaggtt ccccgactcg tggccgcacg
     1501 acccaagagc cacgccccat cgccgcggca ctccccaaat gcgcccgtcc caccgcccca
     1561 ccccgccccc ccctagtgct cccgctccat cgccacgccc tgcatcagct cgcgcagggc
     1621 gtcgatgaag ttgtcgagct catcctcggg aaggcggccg gcggcgcgtt cgcggagcaa
     1681 ctccacgatc tcggtttcgc gggccacgtc gatgatgtcc atggccacgg tgcgcttgac
     1741 gcgcgcaacg gagcggacca ccgaggcccg ttcgacgacc aggtcgatga tgcggcggtc
     1801 gaggtcatcc aggcggcggc gctgctcggt gacctgcgcg gcgatgtcgt tgccggtgat
     1861 ccggctgcgg tcggcagccg tggtacgcgt gcggtcagcg gcagtggagg ctcgacccgg
     1921 gctggtggcg gatgcccgac tcgcacggtc gccccccctg gagctcacgg tccttcccgg
     1981 agctgccttc ttcctcgacg cgtttccctt ttcgccccct cgcgtcaccg acttccgctt
     2041 gccggccttt cccttctcag gcttccgcgt cgtcgccacg gtgcctctcg gtggaatcgt
     2101 cgtcgttctc ggccggctcg agcggctcgc ggaagtagcg ggagtcgacg gcctcgagac
     2161 cttcggggag gccgtgctcg cccgaatcgc gggtctcggg ctcggaatca tcgccgacgc
     2221 cgaaccgacg cccgtcgccc ccgtcggagt cggcccccgc cccaagttcg agcgcgggag
     2281 gctgatctcc ctcgaagtcg atcggttcga tgccggcgcg ctcgaagcgg gccttctcgg
     2341 cgtcgctcca ggccgggttc tttgcggcaa gggcggagat ctcatccagc cgtggcagat
     2401 cggaaagctt gttcaggccg aagtagttca ggaattcctg ggtgctgccg tagagcagcg
     2461 gctttcccgg gccgggatcg cgccccttga tcatgacgag atcgcgttcg agcagcgtgg
     2521 cgagcacgcc gccggcatcc acgccgcgga tccgttcgat ctcgccgcgg gtgatgggct
     2581 gcttgtaggc cacgacggcc acggtctcga gggccgcgcg ggagaggcgc accttccgct
     2641 tccccttcat gaggcgctcc acccagggcg cccacgactc gcgcgtgacg aggacatagc
     2701 cgccggcaac ttcgagcagg gccacgccgc gcctggacgc gtcgtactcg actttcagct
     2761 cttcgaggac aacccgcagc tcggccgccg ccatgttgcc ggtggccttt ttcaggagag
     2821 caaacgtcag cggctcgtcg ctggcgaaca ggagggcctc gaccagcgac ttccgggctt
     2881 cgcgatccgc caggagcgaa gtatcgccag cgtcctcgcc gtttgcggtc acgggatcgg
     2941 cgctcgcggc cacgagttcc tcgccaccct tctcgtccgt cattcccgga tcatccgcgc
     3001 cgacatcatc caacccgccg gcttccaggt catctcgttc catcgggtga ttcctccggg
     3061 ccctggccgt cgctcctctc gcc
//
LOCUS       DASVOD010000180         2282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_376110, whole genome shotgun sequence.
ACCESSION   DASVOD010000180 DASVOD010000000
VERSION     DASVOD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376110"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="VF720_03800"
     CDS             <1..1055
                     /locus_tag="VF720_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878505.1"
                     /translation="QTPSSAQLSFGRPLGTKYGASQIQVFAYKVIDWHGATLTSGVLT
                     KEDAAILNPWLQLRFIGNNNFWGSGEGIMQSMQTEGGLARSFLNNTLGVLQNCTAIRL
                     AGCGGAAVDTTYCIPTSVVAGSHFASTILPRGRGNGCPNLLPFDRMSVNGAVASAKGQ
                     LNYVRLVPGTGGTPTDIGFESVTNLNVIDVLYHTVLDGISVGRMRSNPGYFGVGCDDV
                     GASYARTDNIHDWFGAPVLCKIPQTLVDVPVDGGPPPPAFRHALGNAYPNPMNPTTRI
                     QFTNGTANGRVRVEIFDVTGRLVRSLVDAKMPAGVHEVTWDGANEGGSTVPSGMYFYR
                     MTADDFVAAKKLLVSK"
     gene            1171..1974
                     /locus_tag="VF720_03805"
     CDS             1171..1974
                     /locus_tag="VF720_03805"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7878506.1"
                     /translation="MTAACIRSTVVATLLLLAPARTCFADGPAPDAVPNEAPESGHRL
                     PTRTLLTIGAAAGGLFLATQLDDWAAEEAPEANSRIQQQVSHTARSFGEPIMLGAGLG
                     LVALAGPVLGHKEWTGSALRIGGGVAVAAGSSALLKEAFGRSRPNESTDDGDDLSPFS
                     GHRSFPSGHTTVAFALATGIDQETRAGWVPWVAYPAAALVGWSRVRDNRHGTSDVVAG
                     AILGTVVARHVVKYEQKLTKPGVKSMNVHLDQVGPDGGKAPALGLELRF"
BASE COUNT          374 a          841 c          693 g          374 t
ORIGIN      
        1 accagacgcc ctcctcggcc cagctgtcgt tcggtcgtcc actcggcacg aagtacgggg
       61 ccagccagat ccaggtgttc gcctacaagg tgatcgactg gcacggcgcc acgctcacct
      121 cgggcgtcct gacgaaggaa gacgcggcca tcctcaatcc gtggctccag ctccggttca
      181 tcggaaacaa caatttctgg gggtccggcg aggggatcat gcagtcgatg caaactgaag
      241 gcggcctcgc ccgatcgttc ctgaacaaca cgctcggtgt cctgcagaac tgcacggcaa
      301 ttcggctcgc cggatgcggc ggcgccgccg ttgataccac ttactgcatc ccgaccagcg
      361 tggtggcggg ctcgcacttt gcgagcacga tcctgccgcg cggccgcggg aatggctgcc
      421 cgaacctgct ccccttcgat cggatgagcg tgaacggcgc cgttgcctcc gcaaaggggc
      481 agctcaacta cgtgcgactc gttccgggca cgggcggcac gcccaccgat atcgggttcg
      541 agtcggtcac gaacctcaac gtcatcgacg tcctgtacca taccgtcctc gacggcatct
      601 cggtcggccg catgcgcagc aacccgggct acttcggcgt cggatgcgat gacgtggggg
      661 cctcctacgc ccggaccgac aacatccacg actggttcgg cgcgcccgtc ctctgcaaga
      721 tcccgcagac cctggtcgac gttcccgtcg acggtggccc gccgccgccg gcgttccgtc
      781 atgcgctcgg caacgcctac cccaacccga tgaatcccac cacgcgcatt cagttcacca
      841 acggcacggc caacggccgg gtccgggtcg agatcttcga tgtcaccggc cgcctggtcc
      901 gttccctggt ggatgcgaag atgccggccg gcgttcacga ggtaacctgg gacggcgcca
      961 acgagggggg ctcgaccgtt ccgagcggca tgtacttcta tcggatgacg gcggacgact
     1021 tcgtggcggc aaagaagctg ctggtatcga aatagggcct ccgggagagg accgggaacc
     1081 acctcgccgc ccggtggttc tcggtcctca acccgcccgg cgtacccccg ccggcgggga
     1141 atcccacgaa cagaaccccg ggagccccaa gtgacggccg cctgtatccg atccaccgtc
     1201 gtcgccaccc ttctcctcct cgcccccgcc cgcacctgtt tcgccgatgg ccccgccccg
     1261 gatgccgtcc ctaatgaagc cccggagagc ggacacaggc tcccgaccag gaccctcctt
     1321 accatcgggg ccgccgcggg cggcctcttc ctcgccaccc agctcgacga ctgggctgcc
     1381 gaggaggctc ccgaagccaa ttcccgcatc cagcagcagg tgtcccacac ggcccgcagt
     1441 ttcggcgagc cgatcatgct cggggccggc ctgggcctcg tggcgctggc gggaccggtt
     1501 ctcggtcaca aggagtggac cggaagcgcg ctgcgtatcg gtgggggagt ggccgtggcg
     1561 gccggctcca gcgccctcct caaggaggct ttcggccgca gccgtcccaa cgaatccacc
     1621 gacgatggcg atgacctctc tcccttctcc ggacaccgct cgtttccctc cggacacacc
     1681 accgtggcct tcgccctggc caccggcatc gaccaggaaa ccagggcagg ctgggttccc
     1741 tgggtggcct atcccgcggc ggccctcgtc ggctggtccc gcgtccgcga caatcgccac
     1801 gggaccagcg acgtggtggc cggcgcgatc ctcggcaccg tggtcgcccg ccacgtggtc
     1861 aagtacgagc agaagctcac gaagcccggc gtcaagtcga tgaacgtgca tcttgaccag
     1921 gtggggccgg acggcggcaa ggccccggca ttgggattgg agctccgctt ctaggagcct
     1981 caaaggggcc gccaaatggc accttccgga gttctcccct atattcaata ggctggcgaa
     2041 ttctccctac ccctccggag gtgccgcatt cggaccgttc cgctcgttct cctcggactg
     2101 ttcctactgg ccgatgccgt cgcggctcga accttcgatc gcaccagcga actccgcccc
     2161 gaaatccccg acctgtcgag cgatcggaca ccggccaacc tcaccctctc ctcccacgtc
     2221 ttcaccgaca cggtcagcta tggcggcacc ttctgggccc ccgactcgat gcgctgggaa
     2281 gc
//
LOCUS       DASVOD010000181         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_323970, whole genome shotgun sequence.
ACCESSION   DASVOD010000181 DASVOD010000000
VERSION     DASVOD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323970"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..126
                     /locus_tag="VF720_03810"
     CDS             7..126
                     /locus_tag="VF720_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878507.1"
                     /translation="MAERAYERLISLPLYPRMTEDDLGLVMEAVTKIARAYRR"
     gene            152..1177
                     /locus_tag="VF720_03815"
     CDS             152..1177
                     /locus_tag="VF720_03815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7878508.1"
                     /translation="MSLTPLDAAGTARWAELEAFPPYGFYRDLDDAACRRYRARQLAS
                     MAGNTAHAAFTWHGEGDGAVDSEAAFGLTSLGWDTEQFGVLAGRLDYLIVAPKSGGTP
                     ATDPRNTAANRRVLAVATEAARAMTAEADRRGLKHLSARVDSRELPVLQALERNGFEL
                     VDAILRFSLDASEWGGAPALPAGVKLRDAVESDVEVLGALSAKGFLYDRFHNDPMLAD
                     GVADRVHAAWLANAVRGKAGDGVLVAEVDGRPAGFFILALDKLAGETLGKTIGTLVLI
                     TVDGTLRRRGVALALSLGSIDWLKERGADRFEVGTQLANVPAANVYLKAGFRLVNTSV
                     SLRRMAS"
     gene            1174..>1565
                     /locus_tag="VF720_03820"
     CDS             1174..>1565
                     /locus_tag="VF720_03820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013077034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7878509.1"
                     /translation="MSAPEPLRAVPGGAAAATGTPMAADGVTPRTPAELAEMAAVHHG
                     LKVLAIVPAHNEGETTAEVVKDVQRLAPGVDVVVVDDCSSDKTGEWARQAGARVLPLP
                     VNLGIGGAVQTGFKFAEDHGYDAVIQVDG"
BASE COUNT          217 a          515 c          584 g          249 t
ORIGIN      
        1 ttccccgtgg ccgagcgcgc ctacgaacgg ctgatctcgc tgccgctcta tccccggatg
       61 accgaggacg acctcggcct ggtgatggaa gcggtgacca agatcgcccg ggcgtaccgc
      121 cgctgaccgg ggacggatcg aacccgatcg catgtcgctg acgccgctgg atgccgcggg
      181 aacggcccgc tgggccgagc tcgaggcatt tcccccgtat ggtttctacc gggatctcga
      241 cgacgcggcc tgccgccgtt atcgggcccg gcagctggcc tcgatggccg gtaacaccgc
      301 gcatgccgcg ttcacctggc acggcgaggg ggatggtgcg gtcgattcgg aggcggcgtt
      361 cgggttgacg tccctgggtt gggataccga gcagttcggc gtcctggccg gtcggctcga
      421 ttacctgatc gtggccccga agtcgggcgg cacgccggct acggatccgc ggaataccgc
      481 ggccaatcgt cgcgtgctcg ccgtggccac cgaggcggca cgggccatga cggccgaggc
      541 ggaccggcgg gggctcaagc acctctccgc gcgggtcgat agccgcgagc taccggtgtt
      601 gcaggcgctc gagcggaatg gattcgagct cgtggacgcc attctccgat tctcgctcga
      661 cgcctcggag tggggtgggg cgccggcgtt gccggcgggt gtgaagctgc gcgatgcggt
      721 cgagtccgat gtcgaggtat tgggggcgct gtccgcgaag ggttttcttt acgatcgttt
      781 ccacaacgat cccatgctcg ccgatggcgt ggccgatcgc gtccacgcgg cgtggctcgc
      841 gaatgcggtt cgcggaaagg ccggcgacgg cgtcctcgtg gccgaagtgg acggccgtcc
      901 ggccgggttc ttcatcctgg ccctcgacaa gctggccggc gaaacgctcg gcaagaccat
      961 cggcacgctc gtcctcatca ccgtggacgg cacgctccgc cggcgcggcg tggcgctggc
     1021 gctctcgctc gggtcgatcg actggctcaa ggagcgcggc gccgaccgct ttgaagtcgg
     1081 cacgcagctg gccaatgtgc cggcggccaa cgtgtacctc aaggcggggt tccggctcgt
     1141 gaacaccagc gtgtcgctcc ggaggatggc gtcatgagcg cgcccgagcc gttgcgcgcg
     1201 gtgccgggtg gtgcggccgc cgcgaccggc acgcccatgg ccgccgacgg cgtcacgccg
     1261 cgcacgccgg ccgagctggc ggagatggcc gcggtgcacc atggcctcaa ggtgctggcc
     1321 atcgtgccgg cccacaacga aggggagacg accgccgagg tcgtgaagga cgtgcagcgg
     1381 ctggcgcccg gtgtggacgt ggtggtggtc gacgactgtt cgagcgacaa gaccggggag
     1441 tgggcccgcc aggccggcgc acgcgtgttg ccgcttccgg tcaacctcgg catcggcggc
     1501 gccgtgcaga ccggcttcaa gtttgccgag gatcacggct acgacgccgt catccaggtg
     1561 gacgg
//
LOCUS       DASVOD010000182         4472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_272459, whole genome shotgun sequence.
ACCESSION   DASVOD010000182 DASVOD010000000
VERSION     DASVOD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272459"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            264..1289
                     /locus_tag="VF720_03825"
     CDS             264..1289
                     /locus_tag="VF720_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7878510.1"
                     /translation="MSAPTPEALPPSAQLVQMAMGHLISRIVHLAAKLEIADRLADGP
                     KKAEELAGPTGMHAPSLYRFMRTLGNFGILTESADHRFGLTPLGEAMKKGAPGAAHSS
                     ILTIASDWWFEGFGAMEHSLKTGGTAFEKTLGMPVFDYLARHPDKASLFSDTMIGFHG
                     AEPPAIAEAYDFAGIETLVDVGGASGHMLATLLSRHAKLKGILFDLPHVVRDAPALLE
                     SRGVADRVAIESGSFFEGVPAGADAYLLSHIIHDWNEEQCLTILGHVRKAMRPTSKLL
                     LVEMVLPEGDTPHPGKLLDLIMLVAPGGKERTEKEYAALYQKAGYNLSRVVPTASPVS
                     VVEGVPA"
     gene            complement(1296..2132)
                     /locus_tag="VF720_03830"
     CDS             complement(1296..2132)
                     /locus_tag="VF720_03830"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878511.1"
                     /translation="MKTGVFVLALALMAAPASATVFSFNTGPDLTNNFNRDVRPGDSG
                     THWEPGYGACNGFPASDGGIVYFDDYASSHSIQFKTGPVTLNSFQISSQRFVCGDGVG
                     AANIAANDYLLRLYDAAGNVVHEEFRIIAAGGAWETLTFNIANVSTIWIASTRNATGE
                     FGSGWWPVLDNVTVNAPVQSGVDDPPVSSLTTRVFPNPSPGPFVFEVPTATPGFVTLS
                     IVDAAGRRIREVHAGTLDVGTHRFGWDGRDDAGIATPSGVYYLRCATSDVVQSERLVR
                     LR"
     gene            complement(2326..2670)
                     /locus_tag="VF720_03835"
     CDS             complement(2326..2670)
                     /locus_tag="VF720_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6717 family protein"
                     /protein_id="HEX7878512.1"
                     /translation="MNALTVLTPYKNNGMWVFDDDRTGLVREPFIMGIPRMIDRVVAD
                     IPNAAEGFNLIFSATRFPGAQLKLEWRRADGGGNWYWSADDDMEGWLCPALFRYFDEA
                     PKEIHVRVEAKG"
     gene            3002..>4472
                     /locus_tag="VF720_03840"
     CDS             3002..>4472
                     /locus_tag="VF720_03840"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HEX7878513.1"
                     /translation="MSPTLPARVPSASAAFRFVRIRTFFLLVLASLVAGLFTAAGSPT
                     AAASPDPSDPNCCFYKVQGTVWYDQNGDGIKQGTEPPLAGWTVQALDIANNVVGTTTT
                     NGAGLYNFDVPVACGATFKIKSIVQSGWTQTFPPSNGLHTGTGNGCSEIPLGPYNFGN
                     NQPACAPFTKTYTLDADFNMGTLNGAVTNVNQLELANTPTTWPYAWIANSGDGTLSKV
                     DTNTGNEVARYYTGPPDINLYAGLAPSRTVVDANGNCWVANRNYSGTGPASVTQIALT
                     GGIDWNANSVINTSGDWNSNGVIDPAEILPWGQDERVIRHYQLGAVGEQARGMTLDKT
                     GGLWVGLSVAQTVVKVNPNLSTATYNASQVPSAPPILITVPIGYMPYGLALSPNGKIY
                     LSTGGERAFEIDPGIASGGTGAGPAVTENIYHPNIYNYGLCVDRNCIVWFALTYTNGP
                     NYGCMRWDPTLTLGNPANGWTVSAPGAAGPGRGICVDFNN"
BASE COUNT          801 a         1510 c         1433 g          728 t
ORIGIN      
        1 ccggtgaaag tagtcattcc atttctccag gaacccgtgc tgccggctac ggacgattga
       61 ggctaacacc aagttcacgc gactccgcaa gcccgaattg caggttgatg cttcagattc
      121 gcactctgat actccatcca atcgcctgtt gacatcgttc aatcggtgcg caccgaccca
      181 ggcgtaccta ttcgcctctc aagcccctcc gatctacact ccccgcaccg ttaccgcagc
      241 gtcccgacat caaggagacc gccatgtccg ctcccactcc cgaagccctc cccccttccg
      301 cccagctcgt ccagatggcc atgggccacc tcatctcgcg gatcgtgcac ctcgcggcga
      361 agctcgagat tgccgaccgg ctggccgacg ggccgaaaaa agccgaggag ctggccgggc
      421 cgaccgggat gcacgcgccg tcgctgtatc gcttcatgcg cacgctcggc aacttcggca
      481 tcctgacgga gagcgccgat catcgcttcg gcctgacgcc gctcggcgaa gcgatgaaga
      541 agggcgcgcc gggggcggcg cattcgtcga tcctgacgat cgccagcgac tggtggttcg
      601 aggggttcgg ggcgatggag cattcgctca agaccggcgg cacggcgttc gagaagacgc
      661 tcggcatgcc ggtcttcgac tacctggcca ggcatcccga caaggcctcc ctcttcagcg
      721 acacgatgat cggcttccac ggggccgaac cgccggcgat cgccgaggcc tacgactttg
      781 ccgggatcga aacgctcgtg gatgtggggg gcgcgtcggg ccacatgctg gccacgctcc
      841 tgtcgcgcca cgcgaaactg aaggggatcc tgttcgacct gccgcacgtc gtgcgcgacg
      901 cgccggcgct gctcgagtcc cgcggcgtgg ccgaccgggt ggccatcgag tcgggcagct
      961 tcttcgaagg cgtgccggcc ggcgccgacg cctacctcct ctcgcacatc atccacgact
     1021 ggaacgagga gcagtgcctc accatcctcg gccacgtgcg gaaggccatg aggccgacgt
     1081 cgaaactcct gctcgtcgag atggtcctcc ccgaggggga cacgccgcac ccgggcaagc
     1141 tgctcgacct catcatgctg gtggcgcccg gcggcaagga gcgcaccgag aaggagtatg
     1201 cggccctgta tcagaaggcc ggctacaatc tgtcgcgcgt ggtgccgacg gcctcgccgg
     1261 tgagcgtggt ggagggagtg ccggcgtaag cctcgtcagc ggaggcggac cagtcgctcc
     1321 gattgaacga catccgacgt cgcgcagcgg aggtagtaca cgccggaagg tgtcgcgata
     1381 ccggcatcat cccgtccgtc ccagccgaaa cggtgggtgc cgacgtccag ggtgcccgcg
     1441 tggacctcgc ggatccgacg gccggcggca tcgacgatcg atagcgtgac gaatcccggc
     1501 gtggccgtcg gcacttcgaa cacgaacggt ccgggactcg ggttggggaa gacccgcgtc
     1561 gtcagcgagg acacgggtgg gtcgtcgacg ccggactgga cgggcgcgtt gaccgtgacg
     1621 ttgtccagga cgggccacca acccgaaccg aactcacccg tcgcgttccg ggtgctcgcg
     1681 atccagatgg tcgagacatt cgcgatgttg aaggtcaacg tttcccaggc gccccccgcc
     1741 gcgatgatcc ggaactcctc gtgcacgaca tttccggccg cgtcgtagag ccgaagcagg
     1801 tagtcgttgg cggcaatgtt ggcggcgccc acgccgtcgc cgcagacgaa tcgctgcgaa
     1861 ctgatctgga agctgttgag cgtgaccgga ccggtcttga actggatcga gtgggaggac
     1921 gcgtagtcgt cgaaatagac aatgccgccg tcagaggcgg gaaagccgtt gcaggcacca
     1981 tagccgggtt cccagtgggt tcccgagtct cccgggcgca cgtcccgatt gaagttgttg
     2041 gtcaggtcgg gcccggtgtt gaacgagaag acggtcgcag acgccggtgc ggccatgagt
     2101 gccaatgcca ggacgaatac tccggtcttc atgactcccc ctcgatcgaa tgtgccgaac
     2161 ctgattgccc ctcaccgcgt gaggaaattc gcctcgggtg gcggggcgga cgggtcgcgc
     2221 tgaaacgttt gacggacgat tgcccactcg cgctggatga ggagcacgat ccggcgaaaa
     2281 ctgtcggcct cggccatccg ccgggcgtac tcacatcgga cgcactcatc cctttgcctc
     2341 cacccgcacg tgaatttcct tcggcgcctc gtcgaaatac cggaagagcg ccgggcagag
     2401 ccacccttcc atgtcgtcgt cggcggacca gtaccaattg ccaccgccgt cggcacggcg
     2461 ccactcgagc ttcagttgcg cgccggggaa ccgcgtggcc gagaagatga ggttgaagcc
     2521 ctcggcggcg ttggggatgt cggccacgac gcgatcgatc atgcgcggaa ttcccatgat
     2581 gaacggttcg cgcacgaggc cggtgcggtc gtcgtcgaag acccacatgc cgttgttttt
     2641 gtagggcgtg aggacggtca gggcgttgat ggggttctcc ttgcggcgtc caggtgactc
     2701 gaaggtaccg ggaggcgacc tgtcgtgccg tggagggtca gggcaggacg attcagtgtg
     2761 tgtgcgaacc ttgaaatgtg tcaagcaaca agccggtctg tgtgacacca cggaaagggc
     2821 gacggagcgt cacttccggc ggaccctccc ggattggtcg gccgaccgtg accctgggcc
     2881 ccgctccccg aaattggcgg ttggatgacg cccgccatgc agtaggatgc cgcgatccca
     2941 gtgtcgtttg gcccacggcc gggcggcgcg gacattctcc ctcacctgca tgaggatacg
     3001 catgtcgccc acgctccccg cccgggtacc atcggcctcc gccgcgttcc gcttcgtccg
     3061 catccgcacc ttcttcctcc tcgtgctggc ctcgctcgtt gccggccttt tcacggcagc
     3121 cggctcaccg accgccgccg catcgcccga cccctcagac ccgaactgct gcttctacaa
     3181 ggtgcagggc acggtctggt acgaccagaa tggcgatggc atcaagcagg gtaccgagcc
     3241 gccgctggcc ggctggacgg tgcaggcgct ggacatcgcg aacaacgtgg tcggcacgac
     3301 gacgacgaat ggcgcggggc tctacaactt cgacgtgccg gtggcgtgcg gcgccacgtt
     3361 caagatcaag tcgatcgtgc agagcggctg gacgcagacc ttcccgccga gcaacggact
     3421 gcacacgggc accggcaacg gctgctcgga gattccgctc gggccgtaca acttcggcaa
     3481 caaccagccg gcctgcgcac cgttcacgaa gacgtacacg ctcgacgccg acttcaacat
     3541 ggggacgctg aacggtgcgg tgacgaacgt gaaccagctc gagctggcca acacccccac
     3601 cacctggccc tacgcctgga tcgccaactc gggcgacggc accctctcca aggtcgatac
     3661 gaacaccggg aacgaagtgg cgcgctacta caccggtccg ccggacatca acctgtatgc
     3721 cggcctggcg ccctcccgca cggtggtgga tgccaacggc aactgctggg tggccaaccg
     3781 caattattcc ggcaccgggc ccgccagcgt cacgcagatc gcgctcaccg gcggcatcga
     3841 ctggaacgcc aacagcgtca tcaacacgtc aggcgactgg aacagcaacg gggtcatcga
     3901 tccagcggaa atattgccgt ggggccagga cgagcgcgtc attcgccact accagctggg
     3961 cgcggtgggc gaacaggccc gcggcatgac gctcgacaag accggcggcc tctgggtggg
     4021 cctgtcggtg gcgcagacgg tcgtcaaggt gaaccccaat ctttcgaccg ccacgtacaa
     4081 cgccagccag gtgccgtcgg cgccgccgat cctgatcacg gtacccatcg gttacatgcc
     4141 gtacggcctc gcgctctcgc cgaacggcaa gatctacctt tcgaccggtg gcgagcgggc
     4201 gttcgagatc gatcccggca tcgcctcggg gggcaccggc gcgggcccgg ccgtcaccga
     4261 gaacatctat cacccgaaca tctacaacta cggactctgc gtcgatcgca actgcatcgt
     4321 ctggttcgcg ctcacctaca ccaacgggcc gaattacggc tgcatgcgct gggatcccac
     4381 gctcacgctg gggaacccgg ccaacggctg gaccgtgtcc gcacccgggg ccgccggccc
     4441 cggccgcggc atctgcgtcg acttcaacaa ca
//
LOCUS       DASVOD010000183         4084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_898366, whole genome shotgun sequence.
ACCESSION   DASVOD010000183 DASVOD010000000
VERSION     DASVOD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898366"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4084)
                     /locus_tag="VF720_03845"
     CDS             complement(<1..>4084)
                     /locus_tag="VF720_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878514.1"
                     /translation="TTTSVTLSWTAPGDDGTTGTAASYDLRYSTGSITAANFATANTV
                     SGEPVVGPSGSVLGMTVSGLSSGVQYHFAIKTADEVPNWSTISNTPAGTTQLAVDTTP
                     PSAVANLALGTWTQTSVLVSWTAPGDDNAAGTASSYDLRYSTSAITAANFGSATAVSG
                     EPVPAVAGTSQSMTVSGLTAGTTYFFALKTSDEVPNVSSISNVPTGSTLPPPDVTAPA
                     AVANLALGTWTQTSVLVIWTSPGDDNAAGTASSYDLRYSTSAITAANFGSATAVSGEP
                     VPAVAGTSQSMTVSGLAAGTTYFFALKTSDEVPNTSTISNVPTGATLPSPDTTSPSAV
                     ANLSLGAWTQTSVLVSWTAPGDDNAAGTAASYDLRMSTSVITAANFGSATPVSGEPVP
                     GIAGTSQSMTVSGLTAGTTYFFALKTSDEVPNTSSISNVPSGPTLPFAPVDTTPPAAV
                     ANLALTSSTISSVTVGWTSPGDDANVGTATTYEMRWSTSPITAVNFASANLVSGIPVP
                     LVAGTSQLVAINGLAAGTTIHLALRTADEIPNWSNISNVLVAATLAPPDGTSPAAVGS
                     VASVGVTANSITIDWDAPGDDGMIGTASVYDIRYRTGPITGANFGSCTTTTGEPAPAV
                     AGTTQSFTIGGLASGTTYWIALKTADEVPNWSNLSNVISVTTLVPPDVTPPGSIGNLA
                     AGSWTTNSLWLTWTSTGDDGSTGVASSYDIRVSSTPITAANFDAATPIANEPAPASSG
                     TSQNVQISGLTPGTDYWFAMRVADEVPNWSSISNVATGSTLDPPDATPPGLVTDLSTG
                     TPTVASLVLTWTAPGDDGAAGTATTYDLRRSTSPITAATFATATPLTGEPVPAVAGTV
                     QSMTASGLVAGTTYYFALRTADEMPNWSGIGNVATATTLPPPDVTPPAAVGDLAFGNV
                     ASTSVMFSWHAPGDDGLAGTAAAYDLRWSTGPITASDFDAATPVAGIPAPLASGASQS
                     VTLDDLVPGQPIWAALRTRDEAGNWSALSNVPSTTPPVPADDVPPAAITSLSISAVGP
                     GWVTIGWLASGDDVTTGLGEAYDLRLSASAITAANFGLAEAIPGEPVPGNPGMAQSMT
                     LTGLTAGARYWAAIKSVDESGNWSSISNVLVIDIPSPPDTTPPAEITLTLVRVDTTSA
                     VLAWLAPGDDGAAGRASRYELRRSEAPLDASNFDAGLPVPAPVPGAAGSAESMTATGL
                     AAGSTWWFAIRAIDDADNAGPVSASLRVDTKEPSEPPDTAPPLAPVGLAVVRDPEGAY
                     LSWSPNLEFDLVGYHVYRRPTGGVWQRRTDAPETDLYWHDRLDGRAGSWDYRVTAINV
                     RGEESAPSTIATLVITSPGGDARLVQLAAPAPNPFTESTLI"
BASE COUNT          655 a         1278 c         1505 g          646 t
ORIGIN      
        1 ggatgagggt ggactcggtg aacgggttcg gcgccggtgc ggcaagctgc accagcctgg
       61 catcgcctcc cgggctcgtg atcaccaacg tggcaatggt gctcggcgcg ctctcctcgc
      121 cccgcacgtt gatggccgtg acgcggtagt cccacgagcc cgcccgtccg tcgagccggt
      181 cgtgccagta gaggtcggtc tccggcgcgt cggtccgccg ctgccacacg ccccccgtcg
      241 ggcgacgata gacgtggtag ccgacgaggt cgaattcgag attcgggctc cacgagaggt
      301 aggccccctc cggatcccgg acaaccgcca ggccgaccgg cgccagagga ggcgcggtat
      361 cgggaggttc gctcggttcc ttggtgtcca cgcggagcga ggcgctcacc ggcccggcgt
      421 tgtcggcgtc atcgatggcc cggatggcga accaccaggt ggagcccgcc gcgaggcccg
      481 tggcggtcat cgattccgcc gaaccggccg cacccggcac cggcgccggc accgggagcc
      541 ccgcgtcgaa attcgacgcg tcgagcggag cttcggaacg ccgcagttcg taacggctgg
      601 cgcgaccggc ggcgccatcg tcacccgggg ccagccaggc caggacggcg ctggtcgtgt
      661 ccacgcggac gagggtcagc gtgatctcgg ccggcggtgt cgtgtcgggg ggcgagggga
      721 tgtcgatcac gagcacgttc gagatgctcg accagttccc cgactcgtcc acgctcttga
      781 tggcggccca gtagcgcgcg ccggccgtga ggccggtcag cgtcatcgac tgcgccatgc
      841 cgggattgcc gggcaccggt tcacccggga tcgcctcggc caacccgaag ttcgcggccg
      901 tgatcgcgga agctgaaagt ctcaggtcgt acgcctcgcc caggccggtc gtgacatcgt
      961 cgcccgaggc cagccagccg atcgtcaccc agccggggcc gacggccgaa atcgagaggc
     1021 tcgtgatggc ggccggcgga acgtcatcgg cgggcaccgg gggcgtagtc gaggggacat
     1081 tcgagagagc cgaccagttg ccggcctcgt cacgcgtgcg cagcgcggcc cagatcggct
     1141 ggcctggtac gagatcgtcg agagtcacgg actgggacgc accggacgcc agcggcgccg
     1201 gaatcccggc taccggcgtg gcggcatcga agtcgctcgc ggtgataggg ccggtggacc
     1261 agcggaggtc gtaggcggcc gcggttccgg ccaggccatc atcgcccggt gcgtgccagc
     1321 tgaacatgac cgaggtcgag gccacgttcc cgaaagccag gtcgccaacg gcggcggggg
     1381 gcgtgacgtc gggcggcgga agcgtggtgg ccgtggccac gttgccgatg ccggaccagt
     1441 tgggcatttc gtcggccgtg cgcagcgcaa aataatacgt ggtgccggcc acgaggccgc
     1501 tcgcggtcat cgactgcacg gtgccggcca cggcgggcac cggctcgccg gtcagcggcg
     1561 tggccgtcgc gaaagtggcc gcggtgatcg ggctggtcga gcggcgcagg tcatacgtgg
     1621 tggccgtgcc ggccgccccg tcatcgccgg gggccgtcca ggtaaggacg agcgaggcca
     1681 cggtcggcgt gccggtcgag aggtcggtca cgagccccgg aggtgtcgcg tccggcggat
     1741 cgagtgttga acctgtggcc acgttgctga tcgacgacca gttcggaact tcgtcggcca
     1801 ctctcatggc aaaccagtag tcggtgcccg gcgtgaggcc cgaaatctgc acattctggc
     1861 tggtgcccga cgacgccggc gccggctcgt tcgcgatcgg cgtggccgcg tcgaagttgg
     1921 ccgccgtgat cggtgtgctc gacacgcgga tgtcgtacga cgacgccacg cccgtcgatc
     1981 cgtcgtcgcc cgtcgaggtc caggtgagcc agaggctgtt ggtggtccac gaacccgcgg
     2041 ccaggttgcc gatgctgccg ggaggcgtaa cgtccggcgg caccagcgtc gtgaccgaga
     2101 tcacgttcga aagattggac cagttcggaa cttcgtcggc cgtcttcagg gcaatccagt
     2161 aggtggtgcc cgaagcgagg cccccgatcg tgaacgactg cgtcgtcccg gccacggcgg
     2221 gcgccggctc accggtcgtg gtggtgcacg agccgaagtt ggctccggtg atgggcccgg
     2281 tacgatagcg gatgtcgtac accgaagccg ttccgatcat cccgtcatcg cccggcgcgt
     2341 cccagtcgat cgtgatgctg ttcgccgtga caccgaccga ggccacgcta ccgacggcgg
     2401 ccggtgaggt gccatccggc ggcgcgagcg tggcggccac gagcacgttc gaaatgttcg
     2461 accagttcgg gatctcgtcg gccgtcctca gcgcgaggtg aatcgttgtg ccggccgcga
     2521 gcccgttgat cgcgaccaac tgcgaggtgc ctgcgaccag cggtaccgga atcccgctga
     2581 ccaggttggc cgaggcgaaa ttgaccgccg tgatcggcga tgtggaccag cgcatttcgt
     2641 atgtcgtggc ggttcccacg ttcgcgtcat cgccgggcga agtccacccc acggtcacgg
     2701 acgagatcgt ggagcttgtg agggcaagat tggcaacagc ggccggagga gtcgtatcga
     2761 ccggagcaaa cggaagcgtc gggccggaag gcacgttgct gatcgaggag gtgttcggga
     2821 cttcgtcact ggttttcagg gcgaagaagt aagtcgttcc ggccgtcagg cccgacaccg
     2881 tcatgctctg cgacgtcccg gcgatacccg ggacaggttc tcccgaaaca ggagttgcgg
     2941 aaccgaagtt cgcggccgtg atgaccgacg tcgacatgcg caggtcgtag gatgcggccg
     3001 ttccggccgc gttatcgtcg ccgggtgcgg tccagctcac gaggaccgac gtctgggtcc
     3061 aggctccgag agagagattg gcaactgccg aaggagatgt cgtatcgggc gagggaaggg
     3121 tggcgccggt aggcacgttg ctgatggtgg aggtgttggg gacttcgtcg ctggtcttca
     3181 gggcaaagaa ataagtcgtc ccggccgcaa ggccggagac tgtcatgctc tgcgacgtcc
     3241 cggcgacagc cgggacaggc tctcccgaaa cagcggtagc cgagccgaag ttggcagccg
     3301 tgatcgccga cgtcgagtag cgaaggtcat acgaggacgc cgttccggcc gcgttgtcat
     3361 caccaggaga agtccagatc acgaggacgg acgtctgcgt ccatgtcccg agggccaggt
     3421 tggccacggc tgccggggcg gtgacgtcgg gcggcggcaa cgtcgagccc gttggcacat
     3481 tcgaaatcga cgagacgttg ggtacttcgt cactggtctt cagtgcaaag aaatacgtcg
     3541 ttccggccgt caggccggac accgtcatgc tctgcgacgt cccggcgaca gccgggacag
     3601 gctctcccga aacagcggta gccgagccga aattcgccgc ggtgatcgcc gatgtcgagt
     3661 agcgaaggtc atacgaggac gccgttccgg ccgcgttgtc atcaccaggt gccgtccagc
     3721 tcacgaggac cgaggtctgc gtccatgtcc ccagcgccag gttggccacg gccgacgggg
     3781 gcgtggtgtc gactgccagc tgggtcgtac cggccggcgt gttggagatc gtggaccagt
     3841 tggggacttc gtcggcggtc ttgatggcaa agtgatattg aacgccggac gagagcccgg
     3901 agacggtcat tcccagcacg gagccggagg ggccgaccac aggctcaccg ctgacggtgt
     3961 tggcggtggc gaagttggcg gcggtgatgc tgccggtgga atagcggagg tcgtagctgg
     4021 cggcggtgcc cgtggtgccg tcgtcgcccg gagcggtcca ggacagggtg accgaggtgg
     4081 tggt
//
LOCUS       DASVOD010000184         3521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_585587, whole genome shotgun sequence.
ACCESSION   DASVOD010000184 DASVOD010000000
VERSION     DASVOD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585587"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="VF720_03850"
     CDS             <1..747
                     /locus_tag="VF720_03850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7878515.1"
                     /translation="FIPVVYKDCFFSVLSESTRDDLIGRGVRPDRLRLIHSGMDHRQY
                     TPDGRPPSARPPWVAYLGRLKKYKGIDLVIQALPEVVQRVPDVVYKIVGEGDDRPRLE
                     ALTKQLGMEKHVDFTGWISGATKTDLLRNCRILAYTSPKEGWGLSVIEAGACCVPVLA
                     SNSPGLRESVVDGKTGFLVPHGDVAAIAARMTTLFTDDALADRMGAAGIEWAATFNWE
                     DTTRKTLELLQEAAAERRTEPGSAHVMRAG"
     gene            749..1801
                     /locus_tag="VF720_03855"
     CDS             749..1801
                     /locus_tag="VF720_03855"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7878516.1"
                     /translation="MTSGIPADGPASPKKSPWLKWVLLTAKIALSVALMGWALSRVQL
                     DLFEQRVGSMDMAWMVAAAATFALSNVLGAVQWGWLLRLSGARIPFRKVLSFHWVGLF
                     FSNLLPANVGGDVVRVVDVARTTGSRRGAVGATLLDRLLGFVAIAVLALGALPLLPKG
                     TVSPWLFGVVLAFSLATLFLSLTIFHRGLFAPLERMFGHVQLFSLGRRLTAVADELHA
                     YRRHPGTLLQLLGLALVVQLNRVAVHLLMARSLGIRLSAVYFFVIVPILAIVVVLPVS
                     VAGLGIRETAAVGLFGHVGVSPSDAVAHQLSTFLVCLAVNMIGGVLFVARSFGKSPGT
                     MATAGGPGELAAEDLS"
     gene            1798..>3521
                     /locus_tag="VF720_03860"
     CDS             1798..>3521
                     /locus_tag="VF720_03860"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX7878517.1"
                     /translation="MKERIWPASPEGRWNTIVGAFVFLVSLLVYAWCVTPTVPYWDSG
                     EYIATSYILGIPHPPGTPLYVLIGRLFTFLPLGSIAVRVNLLSSLSSAVAVLLTYLIT
                     VRLVKKNLAGDGSWWMPLAGGVVASFFMAFGTTFWDNAIEAEVYASASAIMCFAIWLA
                     LLWWDGQGEARNDRILWLILYVLFLAIGIHLGTFLVFPCIYLLVTMVHWDRVNKGVFW
                     GTIILFLVAALARTAVVSTIDDVDKRIVDLSFAAPDVQRTAGLLTLVAAAAVVGNLVS
                     VLGLRFTLGIGLLAVIGLSVHFYLLIRAGLNPVINEGDPSTWNSLWLLLSRDQYKPPE
                     AIFRKAGYAYQFSQMYWRYFTWQFHLADFLAGKTFWLPIMVGLFGAFLNFFRNRKSFW
                     LMLTLFLITGPFLVWYLNFKEGEVRERDYFFVVNFHLFAIWIGIGAAALIQWVDELMT
                     AGRERTGRIPPAGVLATTALLVFMSTMPLWAGQENQNFFRHNRRGNWIAHNYAYNMLV
                     GLEKDAILFTNGDNDTFPLWYLQTVERFRPDVRVVCLSLLNTDWCIRQLRDEEPKVPV
                     ELTDAQISR"
BASE COUNT          486 a         1207 c         1140 g          688 t
ORIGIN      
        1 ttcatcccgg tcgtctacaa ggattgcttc ttctcggtgc tctcggagag cacgcgcgac
       61 gacctgatcg gtcggggggt gcgtcccgat cggctccggc tgatccacag cgggatggac
      121 caccggcaat acacccccga cggacggccg ccgtcggccc gacctccatg ggtggcgtac
      181 ctcggtcgac tcaagaagta caaggggatc gacctcgtga tccaggccct gcccgaagtg
      241 gtccagcgcg tccccgatgt ggtgtacaag atcgtgggcg aaggggatga ccggccccgc
      301 ctcgaggcgt tgacgaagca gctcggcatg gaaaagcacg tcgatttcac cgggtggatt
      361 tcgggcgcca ccaagaccga cctcctccgc aactgccgca tcctggccta tacctcgccg
      421 aaggaagggt gggggctgtc ggtcatcgag gccggcgcct gctgcgtgcc ggtcctggcc
      481 agcaactcgc cggggctgcg cgagtcggtc gtggacggca agaccggatt cctggtgccg
      541 catggcgacg tcgcggcgat cgcggcgcgg atgaccaccc tcttcaccga cgacgcgctg
      601 gccgatcgga tgggggcggc cggcatcgag tgggccgcca cgttcaactg ggaggacacc
      661 acgcggaaga cgctcgagct cctgcaggag gcggccgccg agcggcgcac cgagcccgga
      721 tcggcccacg tgatgagggc cggctgaggt gacttccgga atcccggccg acgggcctgc
      781 atccccgaag aagagcccct ggctcaagtg ggtcctgctc accgccaaga tcgccctctc
      841 ggtggccctc atgggatggg ccctttcccg cgtgcagctc gatctcttcg aacaacgcgt
      901 cggctctatg gacatggcct ggatggtggc ggccgccgcc acgtttgccc tgagcaacgt
      961 tctcggcgcc gtgcagtggg gatggctcct gcgcctgtcg ggagcgcgca tcccgttccg
     1021 caaggtgctg tcgttccact gggtggggct cttcttcagc aacctcctgc cggccaatgt
     1081 gggtggggat gtcgtccgcg tggtggacgt ggcccgcacc accgggagcc ggcgcggcgc
     1141 cgtgggagcc acgctcctcg accggctgct cggcttcgtg gccatcgccg tgctggcgct
     1201 cggggcgttg cccctcctcc cgaagggaac cgtgagtcca tggcttttcg gcgtggtgct
     1261 cgcgttcagc ctggccaccc tcttcctttc gctcaccatc ttccaccgcg gcctctttgc
     1321 accgctggag cggatgttcg gccatgtgca gctcttctcg ctcggacggc gcctgacggc
     1381 ggtggccgat gaactgcacg cctaccgtcg gcaccccggc accctgctcc agctcctggg
     1441 gctggccctg gtcgtgcagc tcaatcgggt ggccgtgcac ctcctcatgg cccggagcct
     1501 cggcatccgg ctgtcggctg tctacttctt cgtgatcgtg ccgatcctgg ccatcgtggt
     1561 ggtgctcccc gtgtcggtgg ccggcctcgg catccgcgaa accgcggcgg tggggctctt
     1621 cgggcacgtg ggtgtctcgc cgtccgacgc ggtggcccac cagctctcca cgttcctggt
     1681 ctgcctggcc gtgaacatga tcggcggcgt gctcttcgtg gcgcgttcgt tcggcaagtc
     1741 ccccggcacc atggccacgg cgggcggccc cggcgagctg gccgccgagg acctgtcgtg
     1801 aaggagcgga tctggcccgc ctcgcccgaa gggcgctgga acacgatcgt cggggcgttc
     1861 gtctttctcg tatcactcct ggtgtacgcc tggtgcgtga cgcccaccgt tccctactgg
     1921 gactcgggcg agtacatcgc cacctcgtac atcctgggga tcccgcaccc gcccggcacg
     1981 ccgctctacg tgctgatcgg acgcctcttc accttcctgc cgctcgggtc gatcgccgtg
     2041 cgcgtcaacc tcctgtcgag cctgtcgtcg gcggtggccg tgctcttgac gtatctcatc
     2101 accgtgcggc tcgtgaagaa gaacctggcg ggcgacggca gctggtggat gcccctggcc
     2161 ggcggggtgg tggcgtcctt cttcatggcc ttcggcacca ccttctggga caacgccatc
     2221 gaagccgagg tgtacgcctc ggccagcgcc atcatgtgct ttgccatctg gctggcgctc
     2281 ctctggtggg acgggcaggg tgaggcgcgc aacgaccgca tcctctggct catcctgtac
     2341 gtcctcttcc tggccatcgg catccacctg ggcacgttcc tcgtcttccc gtgcatctac
     2401 ctgctcgtca cgatggttca ctgggaccgg gtcaacaagg gggtgttctg ggggacgatc
     2461 atcctcttcc tggtggctgc gctggcccgc accgcggtcg tgtcgaccat cgacgacgtg
     2521 gacaagcgga tcgtcgacct gtcgtttgcg gctcccgatg tgcagcgtac ggccggcctg
     2581 ttgaccctgg tggccgccgc ggccgtcgtg ggtaacctgg tttcggtgct cgggctccgc
     2641 ttcacgctcg gcatcggcct cctggcggtg atcggcctga gcgtccactt ctacctgctc
     2701 atccgggcgg gcctcaaccc cgtcatcaac gagggggacc cgagcacctg gaacagcctc
     2761 tggctcctgt tgtcgcgcga ccagtacaaa ccccccgagg ccatcttccg caaggccggc
     2821 tacgcctacc agttcagcca gatgtactgg cgctacttca cctggcagtt ccacctcgcc
     2881 gatttccttg ccggcaagac gttctggctt ccgatcatgg tgggcctctt cggcgcgttc
     2941 ctgaacttct tccgcaacag gaagtcgttc tggctgatgc tcacgctctt cctgatcacc
     3001 gggccgttcc tcgtgtggta cctgaatttc aaggaagggg aggtccggga gcgcgactat
     3061 ttcttcgtgg tgaacttcca tctcttcgcc atctggatcg gcatcggagc ggcggccttg
     3121 atccagtggg tggatgagtt gatgactgcg ggccgggagc gcaccggccg gattcctccc
     3181 gcgggggtcc tggccaccac cgccctgctg gtcttcatgt cgaccatgcc gctctgggcc
     3241 gggcaggaga accagaactt cttccggcac aatcgccgcg ggaactggat tgcccacaac
     3301 tacgcgtaca acatgctggt cgggctcgag aaggacgcca tcctcttcac caacggggac
     3361 aacgacacgt tcccgctctg gtatctccag accgtcgagc gtttccggcc ggacgtacgc
     3421 gtcgtctgcc tctccctgtt gaataccgac tggtgcatcc gtcaactgcg cgacgaggag
     3481 ccgaaggtgc cggtggagct cacggacgcc cagatcagcc g
//
LOCUS       DASVOD010000185        12156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_12614, whole genome shotgun sequence.
ACCESSION   DASVOD010000185 DASVOD010000000
VERSION     DASVOD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12156
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12614"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VF720_03865"
     CDS             <1..1703
                     /locus_tag="VF720_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878518.1"
                     /translation="ESCDFIRETVTIVPGEEPCGPVGSVCGPLSFFGPSADTTCPGEP
                     LSVTFTVENQGEPNVDVYFRTSSTGWTLSPTFINNLDTGTRQVIATRTNPSSTTQQLV
                     VRATSYLATTTPADTSGESCDFVRETVTIVPGEEPCGPVGSVCGPLSFIAPPADTVCP
                     GEPVQFTVTVENQGEPAIDVYLRTASSGWTFSSPVINNLASGATRQITVSRSTVPTSP
                     LELTLRATSYLATSTPADTSGDACDFVREVVNIVIGPCEDLAQVCPRTIGFWRQQTQQ
                     KANGSRKICSDDPSDPTDMQRLWENVIQMTDLTSFRGNDGSTISVSDLQKLEGDDLFD
                     ALGCELEGPRPMTQRNMAEIQYLALLLNVSMGLIDLDTPVTIGEAGRTVGDAIDEIEE
                     ILNRSNSTNVQLGTAGTLAECINSGSCIRGIPCEGGENGPFAGFGRTCPSGNVGFVCQ
                     NGDDDDDDDDDHGNNGKSDHRVGPVPAGQLALSAYPNPFNPTAILRWSVGSDLVGQPA
                     VIDVFDAAGRPVAHLFDGVVSSESNEVLLAPDSWSSGMYIARLTVGNKSIVYRLMLVK
                     "
     gene            1886..2353
                     /locus_tag="VF720_03870"
     CDS             1886..2353
                     /locus_tag="VF720_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878519.1"
                     /translation="MTRILAVILMLSSLLVTVPGRAQFTPGGGGGHGGPGHFGGGDRE
                     EMRTRMEALAALNLEGMWGVLTFGIGLSADQLESLRAPFQVQWDRRAEILKDTDKEDT
                     EGWEAIRDEFKSVREDLETMIKTTIGNEKYKEYSKILKDREKMVRNRTGGGPG"
     gene            2567..5314
                     /locus_tag="VF720_03875"
     CDS             2567..5314
                     /locus_tag="VF720_03875"
                     /inference="COORDINATES: protein motif:HMM:NF025295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEX7878520.1"
                     /translation="MNRILPTVLFVLLALFPAGSARAQCATWLSTPFSGPGYGTDGEV
                     NCFLNWDPDGSGPDPTWLIVGGEFHSVDGVPADNIAAWDGFVWRALGFGTNGPVRALA
                     NWNGQLIVGGNFQFAGNLAVKSVARYTAGAWSPMGNWQGTNNNALVYALAVHNGTLFV
                     GGSLPVAYGATARWNGSDWATSPSGIGSVTCFVQFNGMLLAGGSYGSIEYPAIKQYNG
                     TSWSDFEPALTHNRPHGVESMAVVGTALYVAALTILESSAHITRYDLNFRVWNDLAGG
                     PCYPRVLYDRGGTLYAAGNYCCSSPNSRRVQYYAGNQWWPVGALGPDCATVTPDGVNA
                     LVDYNGLLYAGGEFRVTGGDWPVGIARWNGTTWSVPQSSSFISALRPFGTKMVLGGLF
                     STATPAGQAYHLATWDGNQLASFPGPNAPVYAMYDYTDSSFPFTKHLVIGGGFNRVGN
                     VSTFGIASWNETSDMPPSWSAIGSGFNNTVLVIDSYRGKMVAGGLFTGSGSTPMSRIA
                     RLSSGAWTAMGSGFNGAVRALKYYTDRNTDRAILVAGGDFTMTGSSAVSYISGWSESL
                     SGGPTFTWGPFGGGFNAPVYALEYYNSSLYAAGNFTASTAGTALNRIARSTTSGWVPV
                     GTATTGGGLNGNVTGMEVINGFLYVTGSFTAADGVAAYRIARWDGTSWSGVGGGTSGA
                     VMAVAGYGSEVVAGGDFDQAGSTFTWDIARYSASGAPWVVTQPTAQAVSEGATSFFKI
                     EPASGYSGVTYQWRRNNVAVVNGLTPGGSTITGANQSMILISNTAAADSGNYSCQVSN
                     GCGAAVSGSAKLTVQLVSGVGDLPLGVGRIELAAGRSYAGSAARLAWTLPIAMSVRLD
                     IFDVQGRRLATLAEGPHEAGRHESDWQGTPDGRVPPGIYFARLSGSGAIVTTKVVVTG
                     G"
     gene            5533..11001
                     /locus_tag="VF720_03880"
     CDS             5533..11001
                     /locus_tag="VF720_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878521.1"
                     /translation="MRTESSCTGPTSSAGHWCFRFAALALLLFASSPASASHVNIVVD
                     GNLNDLISVVNANPGGANGGFSAVDPLGDVYTPTCGYVNGYDIRDTYAFFDFRDGAGE
                     PTPHDVTLYLGWVVEGLVGDVDGDGNPDTFSTASPGGSTGCAISDEVGIGPNESYNLL
                     FDLDCTGEVGDVRVAVLNNMVGRFNGTTFQPLPGATFAMSGHNLEVRIPNYQNLLQGL
                     NLTGIDLCDAQVRMTANAEFDGPGEDFVSPPFQLEVPPQVSIGKFPPTQVVCSGQNPE
                     WRVVVNNIGMCQLNEITVTDTLQSGMTFVSSVPPSTGTSQFRTWQFNDIDLAPGDSVV
                     ITLAATFGDQCPAGPLTNTVAVEAVHTSTCLEQPLSVSARDTGTAACRSACLTVRAVD
                     STLGGCPGSDVTAGFYIRNCGTVDAINLGVTTSCGDMSPVDVSTIPTILGAGDSALVQ
                     VTCRLPATCDPAGDYTIRLDGTANIADLECTDQASASAEVDCAAPCLQVEFNRAPAAA
                     CAGDTFAVVFDVRNCSPSTTETVTLTGFCNNVMPVLSVTPPSLVLGPNTQQEVTVRCI
                     MPAECQGDISVRLNASTSVGPGTPGCTAEANQSHTVACLDVCLSATAPNDTTICPGET
                     FLGTFTLRNCNLNTASLRGLVRPVGSRTALGVPAGQHYVLTATCNGTPVTVTPSTVDL
                     AAGAKANVTVSCNMPAECGGPLDIDLKAVAWLLEDPTCRDSTTVDVSVDCAASCVDVV
                     ECPPISACPGETAQVPFVIRNCSKETNETVTVSATCNGTPVVLPITSYTIAAGDSVTV
                     TVPCVMPEVCAGNLNVRLTANSHRAGSPSNICPDTDVANCTVTCGTICVAVNNPAPLE
                     GCPGDTIDVPFTVSNCGTVAVNVNMVAACNGQVVNTVNPFNFDLNPGQSRTSTVTCVI
                     PDTCEPGEFLTVVNSAWARSATGCAMDIAGNTRITCLDCPTLPVCGPLALNAPPADTT
                     CPDNTFQFTFTVENQGEPDVDVYLRTSATGWTFNTPVINNLATGNPITVTATRSSPPA
                     NPEQVVIRATSYLATSTPADTAGEACDFRTDTVNIVLGSPPCVTVSNPTAIEACPGDT
                     VDVAFTVSNCGIVAFNVNMVGSCNGQIVNTVDPFNFNLSPGQSRISTVTCVVPDDAET
                     GEFLTVVNSAWARTAAGCAFDVAGNTRINIGQCPVSAQCGPLALNAPPADTTCPDNTF
                     QFTFTVENQGEPNVDVYLRTSATGWTFNTPVINNLATGNPITVTAIRSSPPANPEQVV
                     IRATSYLATTTPADTAGEACDFRTDTVNILFGTTPCVSVNNPTPVEACAGATVQLPFT
                     VTNCGTVAFNVNMVAACNGQVVNTVTPFNFNLNPGQSQTSTVTCTVPADCKPGEFLTV
                     VNSAWARTAAGCQMDIAGTTRINCVQCPVGGSCGPLALVAPPSDSTCPGQPVQFTFTV
                     ENQGEAAVDVYLKSPGSGWNLSSTVVNNLASGNPVQVTASRASPPASPLQLTLRATSY
                     PASSTPADTAGFACATASDVVSILTDACAVPLAEACPRTIGFWRQQTQQKPNGSRKIC
                     EDDPGDGNDMLRLWREVIASTDVVAFKRNDGSMATTANMRNLGDDDLFDALGCELEGP
                     RPMTQRDMAEIQYLALMLNVSMGLLDHDTPVNNSSFHGTVGDAIDTIEEMLNHSGSSN
                     GQMSLAGGIADAINNGSGVLGIPCAGGENGPFAGFGQSCPSDNAGFVCPDGESASSLN
                     LTSSVPARQLSLSATPNPFNPTALLHWTVGPDKVGQHAVIDIFDAAGRPVAHLFDGSV
                     TAESNEVLLAPDGWSSGMYIARLTVGNESIVYRLMLVK"
     gene            complement(11149..11619)
                     /locus_tag="VF720_03885"
     CDS             complement(11149..11619)
                     /locus_tag="VF720_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HEX7878522.1"
                     /translation="MDAEVWPDERVTGLVEDNFLPARLHVKDDAADFKRFGENFGAHW
                     TPTILFLDPQGIEHYRIEGFLPVDDFLAQLNLGLGHLAFQAGRFDEAGEYFQTVVNEY
                     STTEGAPEGQYWAGVSRYKSSGDAAALAETALAFDARYQDSSWAKKASVWKPAA"
     gene            11860..>12156
                     /locus_tag="VF720_03890"
     CDS             11860..>12156
                     /locus_tag="VF720_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878523.1"
                     /translation="MRHLMLAAVSAFAFAGCVAVVPIDGTPKFTDRAGDRKSGFIPAE
                     YAPYDGVGTASIDGQVIHTRPDGLKFYGTGNKVVLNPVTSYSTEWLDRVVLDGEW"
BASE COUNT         2120 a         4046 c         3910 g         2080 t
ORIGIN      
        1 gtgagtcgtg cgacttcatc cgcgagacgg taacgatcgt gcccggtgag gaaccgtgcg
       61 gcccggtcgg ctccgtctgc ggaccgctgt cgttcttcgg cccgtcggcc gacacgacct
      121 gtccgggtga gccgctttcg gtgaccttca cggtcgagaa ccagggtgag ccgaacgtcg
      181 acgtctactt ccggacgtca tccaccggct ggaccctgag cccgacgttc atcaacaacc
      241 tggatacggg cacgcggcag gtcatcgcca cgcgcacgaa tccgtccagc acgacccagc
      301 agctcgtggt tcgtgccacg tcgtacctgg ccacgaccac cccggccgac accagcggtg
      361 agtcgtgcga cttcgtccgt gagacggtaa cgatcgtgcc cggtgaggaa ccgtgcggcc
      421 cggttggctc ggtctgcgga ccgctgtcgt tcatcgcgcc accggcggac acggtctgtc
      481 cgggcgagcc cgtccagttc acggttaccg tggagaacca gggtgaaccg gccatcgacg
      541 tgtacttgag gactgcgtcg tcgggctgga ccttcagctc accggtcatc aacaacctgg
      601 cctccggtgc gacacgccag atcacggtgt cacgctcgac ggtgcccacc agcccgttgg
      661 agttgacgct gcgcgcaact tcctaccttg ccacgagcac cccggcggac accagcggcg
      721 atgcctgcga cttcgttcgc gaagtggtca acatcgtcat cggaccgtgc gaggacctgg
      781 cccaggtctg tccgcggaca atcggtttct ggcgccagca gacgcagcag aaggccaacg
      841 gaagccggaa gatctgttcg gatgacccgt ccgacccgac cgacatgcag cggctctggg
      901 agaatgtcat ccagatgacc gacctcacgt cgttccgggg caatgatggt tcgacgatca
      961 gcgtgtccga ccttcagaag ctcgagggtg atgacctctt cgacgcgctt ggctgcgagc
     1021 tcgaaggacc gcgcccgatg acccagcgca acatggcgga gatccagtac ctggcgctcc
     1081 tcctgaacgt atcgatgggg ctcatcgacc tcgacacgcc ggtcaccatc ggcgaggccg
     1141 gccgcaccgt cggcgacgcg atcgacgaaa tcgaggagat cctcaacagg tccaattcca
     1201 ccaacgttca gctcgggacc gcgggcaccc tggccgagtg catcaacagc ggttcctgca
     1261 tccgcggtat cccctgtgaa ggtggcgaaa acggcccgtt cgccggcttc ggcaggacct
     1321 gcccgtccgg aaacgtcggc ttcgtctgcc agaacgggga tgatgacgac gatgacgacg
     1381 acgatcatgg caacaacggg aagtcggacc atcgggtcgg accggtaccg gcagggcagc
     1441 ttgcgctgtc ggcctacccg aatccgttca acccgaccgc catcctccgt tggtcggtcg
     1501 gttccgatct cgtcggtcag ccggccgtga tcgacgtttt cgatgccgcc ggtcggcccg
     1561 tggcgcatct cttcgacgga gtcgtttcgt ccgagtcgaa cgaggtgctc ctggcgccgg
     1621 acagctggtc gagcggcatg tacatcgccc ggttgacggt ggggaacaag tcgatcgtct
     1681 accggttgat gctggtcaag taacaggaag catcgttcgg tgtttccagg ccccccggcc
     1741 ttcgggtcgg ggggctttcg tttggggccc gggttcatgt aaccggtccc cggttcggcg
     1801 gtcggatgac gtattcgact ggcgccagga caagcgtggc gcagtcgtcc cgtcgaacgt
     1861 gtttcctcga aaacggagcc cggaaatgac cagaattctt gcggtgatcc tcatgctctc
     1921 cagccttctc gttacggtcc ccggcagggc gcagttcact cccggaggcg gcggcgggca
     1981 cggtgggccg ggtcactttg gcggcggcga tcgcgaggag atgcggaccc gcatggaggc
     2041 cctggcggcg ctgaatctcg aggggatgtg gggcgtgctc acgttcggaa tcggcctttc
     2101 ggccgaccag ctcgagtcgc tgcgggcgcc gttccaggtg cagtgggatc ggcgggcgga
     2161 aatcctgaag gacaccgaca aggaggacac cgaggggtgg gaggccatcc gggacgagtt
     2221 caagtcggtg cgcgaggatc tcgagacgat gatcaagacg acgatcggga acgagaagta
     2281 caaggaatac tcgaagatcc tgaaggatcg cgagaaaatg gttcgaaacc gcacaggtgg
     2341 tgggccgggc tgagccggcg ggcgcccgag aggagattcg tgaccgacaa aatatggagg
     2401 gcatcgctcc ccggcgaaac taggctgtaa gccgtttcac ttcaattagt tggttatttt
     2461 gctgagaacc gctcgtgctt ccggtcggtc gcatgagccg atccggccca acatggcgct
     2521 accccacacg acagcgtggt cgaaccataa tccacagggg tgcctcgtga accggatcct
     2581 tcccaccgtc cttttcgtcc tgctcgctct tttccccgcc ggatccgccc gggcgcaatg
     2641 cgccacctgg ctgtcgaccc ccttctccgg ccccggctac ggcacggatg gggaagtgaa
     2701 ctgcttcctg aactgggatc cggacggttc cggacccgat ccgacctggc tgatcgtggg
     2761 gggggagttt cactcggtcg atggcgtgcc ggccgacaac atcgcggcct gggatggatt
     2821 cgtctggcgg gccctcgggt tcggcacgaa cgggccggtg cgcgctctcg cgaactggaa
     2881 cgggcagctg atcgtcggtg gaaactttca gttcgcgggc aacctcgcgg taaagtcagt
     2941 ggcccgatac acggccgggg cctggtcgcc gatgggaaac tggcagggaa ccaacaacaa
     3001 cgccctggtc tacgccctgg cggtccataa cggcaccctg ttcgtgggcg ggtcgttgcc
     3061 cgtggcatat ggcgcgacgg cgcgctggaa cggatcggat tgggcgacga gcccctccgg
     3121 catcggcagc gtgacatgct tcgtccagtt caacggcatg ctgctcgccg gcggcagtta
     3181 cggatccatc gagtaccccg ccatcaagca gtacaacggc acttcctggt ccgatttcga
     3241 gcccgcgctc acgcacaatc ggccgcatgg cgtcgagtcg atggcggtcg tcggcaccgc
     3301 gctctacgtg gcggcgctca ccatcctgga atcgagcgcg cacatcacgc ggtacgacct
     3361 gaacttccgg gtctggaacg acctggccgg cggaccctgt tacccgcggg tgctgtacga
     3421 ccgaggtggc actctctacg ccgccggcaa ctactgctgc tcgagcccga acagtcggcg
     3481 tgtccagtac tacgccggca accagtggtg gccggtcggc gcgctcgggc ctgattgcgc
     3541 gacggtgacc ccggacggcg tcaacgcgct ggtcgactac aacggactcc tctacgccgg
     3601 cggcgagttc cgcgtcaccg gcggcgactg gcccgtgggc attgcgcggt ggaacggtac
     3661 gacgtggagt gtgccgcagt cctcttcctt catctcggcc ctgcgaccgt tcggcacgaa
     3721 gatggtgctc ggcggtcttt tctccaccgc cacgcccgcg gggcaggcct atcacctggc
     3781 cacctgggac ggaaaccagc tggccagttt ccccggtccg aacgcgccgg tgtatgccat
     3841 gtacgactac accgactcgt cctttccgtt cacgaagcac ctggtgatcg gcggcggatt
     3901 caaccgggtg gggaacgtat cgacattcgg aatcgccagc tggaacgaga cgagcgacat
     3961 gccgccatcc tggtcggcga tcggcagcgg tttcaacaac acggtcctcg tgatcgattc
     4021 ctatcgcggc aagatggtgg cgggcggact gttcaccgga tcggggagca caccgatgag
     4081 ccggatcgcc cggctcagca gcggggcctg gacggccatg ggctccggct tcaacggcgc
     4141 cgtccgggca ctcaagtact acaccgatcg gaacaccgat cgcgccattc tcgtggccgg
     4201 cggagacttc accatgaccg gaagctccgc cgtcagctac atctcgggct ggagcgagag
     4261 cctgtccggc ggccctacct tcacctgggg accgttcggc ggtggcttca acgcaccggt
     4321 ctacgcgctc gagtactaca acagctcact ctacgcggcg ggcaacttca cggcctcgac
     4381 ggcgggcacg gcgctgaacc gcatcgcgcg gagcacgacc tccggctggg taccggttgg
     4441 cacggccacc accgggggcg gattgaacgg caacgtgacc ggcatggaag tgatcaacgg
     4501 attcctgtac gtgaccggct cgttcaccgc ggcggatggc gtagccgcgt atcggatcgc
     4561 gcgatgggac ggcacttcgt ggagcggggt gggtggcgga acgtccggag cggtcatggc
     4621 cgttgccggc tatggcagcg aagtcgtggc cggaggcgat ttcgaccagg ccggaagcac
     4681 gttcacctgg gacatcgccc ggtacagcgc cagcggggcg ccgtgggtcg tcactcagcc
     4741 gacagcccag gccgtgagcg aaggggcaac ctcgtttttc aagatcgaac cggcctccgg
     4801 gtattccggg gtgacctacc agtggcgtcg caacaacgtg gctgtcgtga acgggcttac
     4861 gcccggcggg tcgacgatca cgggggccaa tcagtcgatg atcctcatct cgaacacggc
     4921 cgcggccgac agtggaaatt attcctgcca ggtttcgaac ggatgtgggg ccgcggtgag
     4981 tggaagcgcc aagctcaccg tgcagctggt ttcaggtgtg ggcgatctgc cgctcggagt
     5041 cgggcgcatc gaactggccg cgggccggtc gtacgcgggc tccgccgctc gcctggcctg
     5101 gaccttgccg attgccatgt ccgtgcgcct cgacatcttt gacgtgcagg gacggcgatt
     5161 ggccacgctg gctgaggggc cgcatgaggc gggccggcat gagtccgact ggcagggcac
     5221 gccggatggt cgtgtgcctc cgggaatcta tttcgcacga ctgtcgggga gcggtgcgat
     5281 cgtgacgacg aaagtggtcg tgaccggagg atgagccgaa ttcgtctcgt gctgaaacgc
     5341 acgtgacctt cgcactgggg taaaccccga gatcaattcc ccaatcgtcg atagggcaaa
     5401 cccgcaatcg cacgtttcac acgtgctttc tattgaaacg cttcattctg actcctagtg
     5461 tcggacatcg gccgcaggaa acttccgagc accgacatcc gacaaacgat ggcatcgcca
     5521 tctgggggtc acatgcgcac cgagtcctcc tgtaccggac cgacgtcatc cgccggccac
     5581 tggtgtttcc gcttcgcggc actggctctc cttctcttcg cttcctctcc cgccagcgcc
     5641 tcccacgtca atatcgtcgt tgacggcaat ctcaacgatc tcatcagcgt ggtgaacgcc
     5701 aatcccggcg gggccaacgg cggcttttcg gccgtcgatc cgctcggcga cgtctacacg
     5761 ccgacgtgcg gctacgtaaa cgggtacgac atccgcgaca cgtacgcttt cttcgatttt
     5821 cgcgacggcg ccggggagcc gaccccgcac gacgtgaccc tctatctcgg atgggtggtg
     5881 gaagggctgg tcggggatgt cgatggcgac gggaacccgg acaccttctc gaccgccagt
     5941 cccggcggct cgaccggctg cgcgatcagc gacgaggtcg ggatcgggcc gaacgagtcg
     6001 tacaacctgc tgttcgacct ggactgcacc ggcgaggtgg gggatgtgcg ggtggcggtc
     6061 ctcaacaaca tggtgggccg cttcaacggt acgacgttcc agcccctgcc cggcgcgacg
     6121 ttcgcgatga gcggacacaa cctcgaggtc cgcatcccga attaccagaa cctgctccag
     6181 gggctgaacc tcaccggcat cgacctgtgc gatgcgcagg tgcgcatgac ggccaacgcc
     6241 gagttcgacg gtccgggcga ggacttcgtc tcgccgccgt tccagctcga ggtgccgccg
     6301 caggtgagca tcggcaagtt cccgccgacg caggtcgtct gcagcgggca gaatcccgag
     6361 tggcgcgtag tcgtgaacaa catcggcatg tgccagctca acgagatcac ggtgaccgat
     6421 acgctgcaga gcggcatgac gttcgtgagc agcgtgccgc catcgaccgg cacgagccag
     6481 ttccggacct ggcagttcaa cgacatcgac cttgcgccag gcgattcggt cgtcatcacg
     6541 ctggccgcca ccttcggcga ccagtgtccc gccggtccgc tcaccaacac cgtggcggtg
     6601 gaggccgtgc acaccagcac ctgccttgag cagccgctct cggtcagcgc gcgcgatacc
     6661 ggcactgcgg cctgtcgcag cgcctgcctc accgtccgcg cggtcgactc cacgctcggc
     6721 ggctgccccg gctccgacgt gaccgccggc ttctacattc gcaattgcgg gaccgtcgac
     6781 gccatcaacc tgggcgtcac cacgagctgc ggcgacatgt cgcccgtgga tgtgtccacc
     6841 atccccacca tcctcggggc gggggattcg gcactggtgc aggtgacctg ccggctcccg
     6901 gccacctgcg acccggccgg cgactacacc atccggctgg acggcaccgc gaacattgcg
     6961 gatctcgagt gcaccgacca ggcgtcggca tcagccgagg tggattgcgc ggcgccctgc
     7021 ctgcaggtgg aattcaaccg cgcaccggcg gcggcctgcg cgggcgatac gttcgccgtc
     7081 gtcttcgacg tgcggaactg cagtccgtcg accaccgaaa cggtcacgct gacgggcttc
     7141 tgcaacaacg tgatgccggt cctctccgtg acgccgccga gcctcgtgct cgggccgaac
     7201 acccagcagg aagtgaccgt gcgctgcatc atgccggccg agtgccaggg ggacatctcg
     7261 gtgaggctga acgccagcac gagtgtcgga cccggaactc ccggatgcac ggccgaggcc
     7321 aaccagtccc acacggtggc ctgcctcgac gtgtgcctca gcgccacggc gccgaacgac
     7381 acgaccatct gtcccggcga gacattcctc gggacgttca cgctccggaa ctgcaacctg
     7441 aacaccgcca gcctccgcgg gctggtgcgt ccggtcggca gccgcacggc cctcggcgtc
     7501 cccgccggcc agcattacgt gctgaccgcc acctgcaacg gcacgcccgt taccgtgaca
     7561 ccgtccaccg tcgatctggc agcgggcgcg aaggccaacg tgacggtgtc gtgcaacatg
     7621 ccggccgagt gcggcggccc tctcgacatc gacctgaaag ccgtggcctg gcttctcgaa
     7681 gacccgacct gccgcgactc gacgaccgtc gacgtgagcg tcgactgcgc cgcatcgtgc
     7741 gtggacgtgg tggaatgccc cccgatttcg gcatgccccg gtgagacggc ccaggtgccg
     7801 ttcgtcatcc gcaactgctc gaaggaaacc aacgagacgg tgacggtgag cgccacctgc
     7861 aatggcacgc cggttgtcct cccgatcacc agttacacca ttgcggccgg agattcggtc
     7921 acggtgacgg tgccctgcgt aatgccggag gtttgtgccg gaaacctgaa cgtccggctg
     7981 acggccaatt cccaccgggc cggcagcccc tcgaacatct gtccggacac cgacgtggcc
     8041 aactgcacgg tgacctgcgg aaccatctgc gtggcggtga acaacccggc gccgctcgag
     8101 gggtgcccgg gggacacgat cgacgttccg ttcacggtga gcaactgcgg cacggtggcg
     8161 gtcaacgtga acatggtggc cgcctgcaac ggccaggtcg tgaacacggt gaatccgttc
     8221 aacttcgacc tgaaccccgg ccagtcgcgg acgagcacgg tgacgtgcgt gatccccgat
     8281 acctgcgagc cgggcgagtt cctgacggtg gtcaacagcg cctgggcccg gtcggcgacc
     8341 ggatgcgcca tggacatcgc ggggaacacc cggatcacct gcctcgattg tccgacgctg
     8401 cccgtctgcg gcccgctggc actgaatgcg ccgcccgccg acaccacatg cccggacaac
     8461 acgttccagt tcaccttcac cgtcgagaac cagggtgagc ccgacgtcga tgtctacctg
     8521 aggacctcgg cgaccggctg gaccttcaac acgccggtga tcaacaatct ggccacggga
     8581 aatccgatca cggtgacggc cacgcgctcg tcgccgccgg ccaacccgga acaggtcgtc
     8641 atccgtgcca cgtcgtacct ggccacttcc accccggccg acacggccgg ggaggcgtgt
     8701 gacttccgca ccgacacggt caacatcgtc ctcggttcgc cgccctgcgt gacggtcagc
     8761 aacccgaccg ccatcgaggc ctgccccggc gataccgtcg acgtggcgtt cacggtgagc
     8821 aactgcggca tcgtggcctt caacgtgaac atggtcggct cgtgcaatgg gcagatcgtg
     8881 aatacggtcg acccgttcaa cttcaacctg agtcccggcc agtcgcgcat cagcacggtg
     8941 acgtgcgtgg tgccggacga tgccgagacc ggcgagttcc tgacggtcgt caacagtgcc
     9001 tgggcgcgaa ccgccgcggg ttgcgcgttc gacgtggccg gaaacacccg gatcaacatc
     9061 ggtcagtgcc cggtcagcgc ccagtgcggt ccgctggcgc tgaacgcgcc gcccgccgac
     9121 accacctgcc cggacaacac gttccagttc accttcaccg tcgagaacca gggcgagccc
     9181 aacgtcgatg tctacctgag gacctcggcc accggctgga ccttcaacac gccggtgatc
     9241 aacaacctgg ctacgggaaa tccgatcacg gtgacggcaa tccgttcgtc gccgccggcc
     9301 aacccggaac aggtcgtcat ccgtgccacg tcgtacctgg ctaccaccac tccggccgat
     9361 actgcggggg aggcgtgcga tttccgcacg gataccgtca acatcctctt cggaacgacg
     9421 ccgtgcgtgt cggtcaacaa cccgactccg gtcgaggctt gtgccggcgc cacggtccag
     9481 cttccgttca cggtcaccaa ctgcgggacc gtggcgttca acgtgaacat ggtcgcggcc
     9541 tgcaacggcc aggtcgtgaa cacggtcact ccgttcaact tcaacctgaa ccccggccag
     9601 tcgcagacca gcacggtgac gtgcacggtt ccggcggact gcaagccggg tgaattcctg
     9661 acggtggtca acagcgcctg ggccaggacg gccgcaggct gccagatgga catcgccgga
     9721 accacccgga tcaactgcgt ccagtgcccg gtcggtggct cctgcggacc gctggccctg
     9781 gtggctccgc cgtccgacag cacctgtccc ggtcagccgg tccagttcac cttcacggtc
     9841 gagaaccagg gtgaagccgc cgtcgatgtc tatctcaaat cgccgggctc cggctggaac
     9901 ctgagctcga cggtggtcaa caaccttgcc tccggcaatc cggtgcaggt gacggcttcc
     9961 cgtgcatcac cgccggccag cccgctgcag ctgacgctgc gcgccacgtc gtacccggcc
    10021 agctcgacac cggccgacac ggccgggttc gcctgcgcca cagccagcga cgtggtcagc
    10081 atcctgaccg atgcctgcgc cgtcccgttg gccgaagcgt gtccgaggac gatcggcttc
    10141 tggcgtcagc agacgcagca gaagccgaac ggcagccgga agatctgcga ggacgatccg
    10201 ggtgacggca acgacatgct gcggctgtgg cgcgaggtga tcgcctcaac cgatgtggtg
    10261 gcgttcaagc gcaacgatgg ctcgatggcc accacggcca acatgcgcaa tctcggtgat
    10321 gacgacctgt tcgatgccct ggggtgcgag ctcgagggtc cccgtcccat gacgcagcgc
    10381 gacatggcgg agatccagta cctggccctc atgctcaacg tctcgatggg tctgctcgac
    10441 cacgacacgc cggtcaacaa cagttcgttc cacggcacgg tcggcgacgc gatcgacacg
    10501 atcgaggaga tgctcaacca ctccggctcc agcaacgggc agatgtcatt ggccggcggc
    10561 atcgccgatg ccatcaacaa cggcagcggt gtcctgggca ttccgtgtgc cggaggcgag
    10621 aatggtccgt tcgcgggctt cggccagagc tgtccgtccg acaacgccgg gttcgtctgt
    10681 ccggacggcg agtccgcgtc gagcctgaac ctcaccagtt cggtgccggc acgccagctc
    10741 tcgctgtccg caacacccaa tccgttcaac ccgaccgccc tgctccactg gacggtcggt
    10801 ccggacaagg tcggtcagca cgccgtgatc gacatcttcg acgcagccgg tcgccccgtg
    10861 gcccacctgt tcgacggaag cgtgacggcc gagtcgaacg aggtgctcct ggcgccggat
    10921 ggctggtcca gtggcatgta catcgcccgg ctcaccgtcg gcaacgagtc gatcgtgtat
    10981 cggttgatgc tcgtgaagta gcccagcggc agggtcggcc cggttcgatt gggccggccc
    11041 tgcacttgat ttgaaatgac gagccctccg ccgaccggcg gagggctcgt tttccttaat
    11101 cagcgaaaac ctgtcggtta cgattcggcc agctcccgct gacccgtctt atgccgccgg
    11161 tttccagacc gacgccttct tcgcccagct ggaatcctgg tagcgggcat cgaaggccag
    11221 ggcggtttcg gccaatgccg cggcatcccc ggatgatttg tagcgcgaaa ctccagccca
    11281 gtactgcccc tcgggggcgc cttcggtcgt ggagtattca ttcaccaccg tctggaaata
    11341 ctctccggcc tcgtcgaagc gaccggcctg gaaggccagg tgaccgagcc ccaggttcaa
    11401 ctgcgccagg aagtcatcga ccggcaggaa gccttcgatc cggtagtgct caatgccttg
    11461 gggatcgaga aagagtatcg tcggcgtcca gtgcgcaccg aagttctcgc cgaagcgctt
    11521 gaaatcggca gcgtcatcct tcacgtgcag gcgggcgggt agaaaattat cttcgacgag
    11581 cccggtgacg cgctcgtcgg gccaaacctc ggcatccagc cgggcacagc cccctcacat
    11641 gggggtggcg ttgaaatcca gcaggatggg gcggttctgg agcctggcgg tgtcgagcac
    11701 cgcatcgacg tcgtgacgcc attgaatcgg catgatgtcc tcccgtgaat gatgtgggag
    11761 cgatcacgcg agtacgtcca gtctggaccc ggggctttga tcggcacaag ctgaaagtcc
    11821 attgggggaa cgcctgatgg cgagtagtat cgccccgtca tgcgacacct gatgcttgcc
    11881 gccgtctcgg cgttcgcttt cgccgggtgc gtggccgtcg ttcccatcga cgggacgccc
    11941 aagttcaccg atcgcgccgg tgaccggaag agtgggttca tccccgccga gtacgcacct
    12001 tacgacggag tcggcacggc ctccatcgat ggccaggtga ttcacacccg ccccgatggg
    12061 ttgaagtttt acggtaccgg caacaaggtg gtgctgaacc cggtgaccag ctattcgacc
    12121 gaatggctcg accgcgtcgt cctcgacggc gaatgg
//
LOCUS       DASVOD010000186        10096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_324356, whole genome shotgun sequence.
ACCESSION   DASVOD010000186 DASVOD010000000
VERSION     DASVOD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324356"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VF720_03895"
     CDS             <1..1466
                     /locus_tag="VF720_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7878524.1"
                     /translation="SNRYITDRFLPDKAIDLVDEAASKIKMEIDSMPVEIDQIGRRLQ
                     RLEVEREALRKEKDAGTKARLQALESEIETLRGEKARLESHWTKEKEAIDRIRTAQEA
                     LEVARGEEARAEREGNLQRVAEIRYGTIPNLEKQLADTTAALNALQSGVKMLKEEVDE
                     EDVAEVVARWTGIPVSRMLEGEVQKLLKMEERLKERVVGQDAAVIAVSDVIRRARAGL
                     QDPRRPLGSFLFLGPTGVGKTELAKALAEFLFDDEQALVRIDMSEYMERHTTSRLIGA
                     PPGYVGYEEGGQLTEAIRRRPYAVILLDEIEKAHPDVFNVLLQLLDDGRLTDNQGRTV
                     SFRNTVVIMTSNLGSAIIQEHAGDKAAIEREVQSLLKQSFRPEFINRIDEVVIFDNLG
                     LDQLKKIVDIQLVDLQERLAERNVGLVLEDPVKELLAREGFDPVYGARPLKRVVQKRL
                     ANPLAQKILAGEVPDGGTVTARLEKGEVVFEAGRGPG"
     gene            complement(1597..2112)
                     /locus_tag="VF720_03900"
     CDS             complement(1597..2112)
                     /locus_tag="VF720_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878525.1"
                     /translation="MKRIVPTALAGALLLVMGGPLVVGGMLVLGGPFVAAAEAGTYPM
                     VRTGSTASLPHVGTASSVRGATGTSTVPAAVLARESGLTLGKHYLEVSPGIWVPVNGE
                     RERRNVRYARLVAFWSDDQRDVAVDEGFPHHRWRDLYDGHYTETWFYPDVNRQYVFDV
                     ESGGLIKTTRR"
     gene            complement(2160..3590)
                     /locus_tag="VF720_03905"
     CDS             complement(2160..3590)
                     /locus_tag="VF720_03905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7878526.1"
                     /translation="MKRKPIPFDQLLSTVLPVDRLPLMDQADIRAALIEGDPIRIEKV
                     ALEVIRTLARLGHLRPLEETREGEERVQRYRDLVSANTITIRIPVAPAGDGVFRLPLP
                     LRDWKGTTSLDEIRSVLALYNRILARDSSLLAGVPEILRQIIQATRSVLGCDQARFVP
                     VREAPGVEGTLDPEYLAEPWDPYIVREWVVSRNHLVHVPELTAQARASGRVPAGMSSL
                     ALVPLGDPTSPVQGAIQAWSARPRFFGEDRLGLFALLSECGSDILSRAAILADLVFVD
                     SSTKIYNRSYFNLQLENEIARARRENKSLALIIFDIDDFKRFNSEYGYEGGNDVLARV
                     ARHLKSGLRPFDSVARWGGEEFALILTAPIGIEHARAVTGRLRRGVERQSFDITGLEG
                     SSHAVTITVSGGGALYPDDAGSGPDLWRAANAALVWSKEHGKNRVTFFNDLSGGSRSA
                     SPEPTGDAPPGDARRDGTTVSKKKGT"
     gene            4094..4447
                     /locus_tag="VF720_03910"
     CDS             4094..4447
                     /locus_tag="VF720_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878527.1"
                     /translation="MRSRSKWVVVLGVTALLTASQASFDGLGPWANETPGSEGEVSLG
                     DRRPVPDGSGNGSGGGQTEGIRQGADPNDFSVSSPRRPNVSTSATPERRDLGSQLRIW
                     FQAMLNRFGGLVHRN"
     gene            4615..7653
                     /locus_tag="VF720_03915"
     CDS             4615..7653
                     /locus_tag="VF720_03915"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7878528.1"
                     /translation="MTDKIDDPVSTADADQQVAAGRPDEALDSLRRLLSRQGLKPAET
                     VLLKRKIAGLLSTQAKWEEARSLLIEARDLAARSGLAAEEALLLVEFGKLHMVRGEID
                     RALECARGAMERAEAAGETLSKAYAENLTGYALFSRGLLDQSQAAFERALMACKASGN
                     LRALSLVYNNLGLLYKERCQWKKALEHFQVAGNLNAIDGAESERRKGLQNLGLVHLRL
                     GQWTDARACFDESLAIGRKLGDPLSVIRALLGLAQLNLSAGQADHAKDQLEQSLELCT
                     QDAYPRETALTHRSLGVMHLKAGDYERARRCFETAWKRAVEIAPEGDLAVELLLHRAE
                     LSLAVGKAREAQSLAQRSHALAMKAGDAFYEAEAFRVLGNVMWALGNLDEAENHFKEG
                     ITAFRSIDAPYPLALVLLDMGRLGAERLTARQEPEKVLNALDEALRLFTQLGVGSLRG
                     ETLLALARAHVRSAAFDRASACLAEATEAYRGLDLPTGMSRVAEVQTELEGRFVDESV
                     SHLNEFRATNRIEEILEARADATTKIGQIVEVVSNAVEADGILLISGLGQRDAEPLAF
                     RGMSPPVARSIGEQLHALHGTAIGTRPQIVLNTEACTVPELSDLAVRHQAQSLILIPI
                     RWDDGREALLYLDRTRTGRRGPFRQSNLNLTVVLSSYLHDLLAQMDRDVTISENLRIK
                     SQLEEKIALADVVTQNRDMLGILKLVEKINRSDLTVLLQGETGTGKTLLAKSIHLSSP
                     RGKGPFVTVDCAALPDNLLESELFGYVKGSFTGAMSDKKGLFEEADGGTIFLDEIGRA
                     GLTVQRRLLHLLDKGEVRPVGSNSYRKLDVRVICATSSKDLRHDVAQGAFIKDLYYRL
                     NDISVVVPSLRDRVEDIPLLADYFLSTFTGETDRDVPGFTRAALNRLLRYNWPGNVRE
                     LEKVVRRAAILCDEGAPINVEHLPEDLLGTAVDEPAILTDGASIHDTVEEMERRMVLK
                     ALEENNWNKSRAAVALGLSRKGLKNKIARYDLDRRIVTRR"
     gene            7688..9796
                     /locus_tag="VF720_03920"
     CDS             7688..9796
                     /locus_tag="VF720_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX7878529.1"
                     /translation="MPGVVGAPVVRSITGRASRSERNRPIPALLRLLLFGACCLGGVL
                     LVGAPMAWADPPLPDNSPAVLAARRALKANPESKERWLELAHVCWNLGTIEGRRAADE
                     VLEDARRKWPHDSEILLDWADLNRERRFISRSRGLYRRALKEEPGSERALTGLSALAM
                     YDWRRYIRPKSLIEADLRAGQLMAVSPDNADGMALGAFTRLMRGDTTEAMARTEALLA
                     RHPDDPRGHFLKLTLDVDAGRWDDAAEHAELALARLTDAAEIEAYRSLENIHWGQEDR
                     RQELPDSLKVRFERNFWKRRDPTPAPVRTERRLEHLRRVFMSDELYGIPETGLRGWDT
                     DPGEAVIRFGMPKARMLGTGVQVSYSFRADRLTTVHNIDGYDYDFNFDDEMLNGNWVE
                     PSGFLTPSLMDKVAAVKEGWMPDPIGGPVLDMVFDAVQFRGKNGWTRVEAIVAAPAVP
                     DTATSWERPIALYTDEWELVAQRGGTLSHAARVKDRQRPDAGWLVDLVPIEVDPGIDS
                     VFIGSELVAGLKMGHGERFDRLLLRQFHDDSLEVSDLMLLDRIDPDLVEGPFARRDGG
                     TVPSPDAIYAIGESVPVYFEAYGLTPDSDGRCRYAVTVEINDLKTFGEWESKRRSALT
                     ANRRPRRPRSTSRFEEWSPTPDIERMIDLSVGRLPAGDFWVKIVVEDLVRSRRAQGEG
                     AFRMVPAPKSKHGDKHGKTP"
     gene            9793..>10096
                     /locus_tag="VF720_03925"
     CDS             9793..>10096
                     /locus_tag="VF720_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878530.1"
                     /translation="MNRLSTGTAWLIGTAALLVVGVALIVAGTRPPSPARDRTTDRTS
                     PATSAAPPNSATPAGSVAHETTPVATPTGSLVRADSTNTPPALLAALAGTSGGDPAL"
BASE COUNT         1720 a         3394 c         3441 g         1541 t
ORIGIN      
        1 tctccaaccg ctacatcacc gaccggtttt tgcccgacaa agccatcgac ctcgtcgacg
       61 aggcggcctc caagatcaag atggagatcg actcgatgcc ggtcgagatc gaccagatcg
      121 gccgccggct ccagcgcctc gaggtcgagc gcgaagcgct tcgcaaggag aaggatgccg
      181 ggacgaaggc gcggttgcag gcgctggaat ctgaaattga aacactccgc ggcgagaagg
      241 cgcgcctcga atcgcactgg acgaaggaaa aggaggccat cgaccggatc cgcaccgccc
      301 aggaggcgct cgaggtggcc cgcggcgagg aggcccgcgc cgagcgggaa ggaaatctcc
      361 agcgcgtggc cgaaatccgc tacggcacga tcccgaacct cgagaagcag ctcgccgaca
      421 cgaccgccgc cctgaatgcc cttcagtccg gcgtgaagat gctcaaggag gaagtcgacg
      481 aggaggacgt ggccgaggtg gtggcccgct ggaccggtat cccggtgagc cggatgctgg
      541 aaggggaggt gcagaagctc ctcaagatgg aggagcgact gaaagagcgg gtggtggggc
      601 aggacgcggc cgtcatcgcc gtctccgacg tcatccgacg ggcccgcgcc gggttgcagg
      661 atccgcgccg gccgctcggc tcgttcctct tcctggggcc taccggtgtc ggcaagaccg
      721 aactggccaa ggcgctggcc gaattcctgt tcgacgacga gcaggcgctg gtgcggatcg
      781 acatgtccga gtacatggag cgccacacca ccagccgcct gatcggtgcc ccgcccggct
      841 acgtgggata cgaggaaggc gggcagctca ccgaagccat ccgccggcgc ccgtacgccg
      901 tcatcctgct cgacgagatc gagaaggcgc accccgacgt cttcaatgtg ctgctgcagc
      961 tgctggacga cgggagactg accgacaatc agggacggac ggtgagtttc cgcaacaccg
     1021 tcgtcatcat gacgtcaaac ctcggcagtg ccatcatcca ggagcacgcc ggcgacaagg
     1081 ccgccatcga gagagaggtg cagtcgctcc tcaagcagtc gttccgcccc gagttcatca
     1141 accggatcga cgaggtggtc atcttcgaca acctcggcct cgatcagctg aagaagatcg
     1201 tcgacatcca gctggtcgac ctccaggagc ggctggccga gcggaacgtt ggcctcgtgc
     1261 tcgaggatcc ggtcaaggaa ctgctggccc gcgagggatt cgaccctgtg tacggcgcaa
     1321 ggccgctgaa gcgcgtcgtc cagaagcgcc tggcgaaccc gctggcccag aagatcctgg
     1381 ccggcgaggt gccggacggc ggcaccgtga cggcacgact ggagaagggg gaggtggtgt
     1441 tcgaggcggg gcgggggccg gggtgagctg tcggtccacc ctgaacgaca aacagccgac
     1501 tgttgccagc aacagtcggc tgtttgcgaa acaggtctgg agtacgggcc ctgccgaacc
     1561 gcccgaccgc gagtgtcgcc gcggcgggcg ccgcggctac cgccgcgtcg tcttgatcaa
     1621 tccgcccgat tccacgtcga acacgtactg gcggttcacg tcggggtaga accaggtctc
     1681 cgtgtaatgc ccgtcgtaga ggtcccgcca ccggtggtgg gggaagcctt cgtcgacggc
     1741 cacgtcacgc tggtcgtccg accagaaggc cacgaggcgg gcgtagcgga cgttgcgccg
     1801 ttcgcgctcg ccgttcaccg gcacccagat gccgggactg acttccaggt agtgcttgcc
     1861 gagcgtcaga ccggactcgc gggcgagcac cgcggcgggc acggtgctcg tgccggtcgc
     1921 gccgcgcacg ctcgaggcgg tgccgacgtg cggcaacgag gcggtggagc cggtgcggac
     1981 catcgggtag gtgccggcct cggcggcggc gacgaacggt ccgcccagga cgagcatgcc
     2041 gcccacgacg agcggtccgc ccatcaccag caggagcgcg ccggcaaggg cggttgggac
     2101 gatgcgtttc attgctgatt tccccccagg gaaacgggcg cgtggccctt cttctcgggc
     2161 taggtgccct tcttcttgga gacggtcgtt ccgtctctcc tggcatcgcc gggcggcgcg
     2221 tcaccggtgg gctccggtga ggcggaccgc gatcctcccg acaaatcgtt gaagaacgtg
     2281 acccggttct ttccgtgttc cttggaccag acgagcgcgg cgttggcggc ccgccacagg
     2341 tcgggtccgc tcccggcgtc gtccggatag agagcgccgc cgcccgacac ggtgatggtg
     2401 accgcgtggg aggagccctc gagcccggtg atgtcgaatg actgtcgttc cacgccgcgg
     2461 cgcaggcgcc cggtaacggc gcgggcgtgc tcgatgccga tgggcgccgt caggatgagc
     2521 gcgaattcct cgccgcccca gcgcgccacg gagtcgaacg gccggagccc cgacttcagg
     2581 tggcgcgcca cgcgagccag gacatcattg ccaccctcgt agccgtattc gctgttgaag
     2641 cgcttgaagt cgtcgatgtc gaagatgatc agcgcgaggc tcttgttctc gcgccgggcg
     2701 cgggcgattt cgttctcgag ctgcaggttg aaatacgacc ggttgtagat cttggtcgag
     2761 gaatccacga agacgaggtc ggccaggatg gcggcgcggc tgaggatgtc cgaaccgcat
     2821 tcggaaagga gcgcgaagag gccgagcctg tcctcgccga agaagcgggg gcgggcgctc
     2881 caggcctgga tggccccctg caccggcgag gtgggatcgc ccagcgggac gagcgccaga
     2941 gaggacatgc cggcgggaac gcgccccgaa gcgcgcgcct gcgccgtgag ttccggcacg
     3001 tgcaccaggt ggttccgcga cacgacccac tcgcgcacga tgtacggatc ccacggctcg
     3061 gcgagatact cgggatcaag cgttccctcg acgccgggcg cctcgcggac cggcacgaac
     3121 cgcgcctggt cgcagccgag cacgcttcgc gtggcctgga tgatctgccg caggatttcc
     3181 ggcacgccgg cgaggaggct cgaatcgcgg gccaggatgc ggttgtagag ggcgagcacc
     3241 gaccggattt catcgagcga cgtggtcccc ttccagtcgc ggagcggcaa cggcaggcgg
     3301 aacacgccgt cgccggccgg cgcgacggga atgcggatgg tgatcgtgtt ggcgctgacg
     3361 agatcgcgat agcgctgaac gcgctcttcg ccctcgcggg tttcctcgag gggacgcagg
     3421 tgaccgagcc gggccagcgt gcggatgacc tccaacgcca ccttctcgat ccggatggga
     3481 tcgccctcga tcagggcggc ccggatatcg gcctgatcca tgagcggcag ccggtccacc
     3541 ggtagaacgg tcgacaggag ctggtcgaag gggatcggct tccgtttcat acccgcgcgg
     3601 tcgggtcctc tcggggtgtc gtgcaggatg cgctggaccg ggctgtcagg ccgtattagg
     3661 ggggtcggca tttttccacc tggatagcgg ccggacaggg aatcaggcct caattatagc
     3721 tggatcgtct gcgcatccgg aactttcatt aaaacaacgg gatagatacc ctcgtccatt
     3781 gcgccaacgg acccagaatc ggacccgcgg tgcctgttcg gatcgcggta tttttgctgg
     3841 gtaccgagta tccatttgca agtatttaca agccaacgag ttgctgatta tcgcatcccc
     3901 gctgtatact gaggtggcaa ggtcggaacc agcccgtgga aacaccggcg gttccgccgg
     3961 cgccaccgac gacgcaaccc cttgcatgac agtcgattag gtggcccgtt ccgttctgcg
     4021 ccacatggca cgggacgtgc ttccttcatc gtaaaggtct ccgcatcctc tgaggtgcat
     4081 gaaggagtga aacatgcgta gtcgttcgaa gtgggtggtc gttctcggcg tgaccgctct
     4141 cctcaccgcc agccaggcca gtttcgacgg gctgggtccg tgggccaacg aaaccccggg
     4201 cagtgaggga gaagtctcgc ttggcgatcg ccgtcccgtc ccggacggga gcggtaacgg
     4261 atcgggtggc ggccagacgg agggaatccg tcagggtgcc gatcccaacg atttcagtgt
     4321 ctcgagcccg cgccggccga atgtctcgac ctcggccacg ccggagcgcc gcgacctggg
     4381 atcccagttg aggatctggt tccaggccat gctcaaccgg ttcggggggc tggtgcaccg
     4441 gaactgatcg gtgcacctcc cttccggccg cggccgagat gtcgcggcga aaggtgaacg
     4501 aagcaaacag ccagagccgt gtccaaggat cgctgcctgc attccttcct ggagaagtga
     4561 atggtcccca ccaatgacga ccggccgccc gcccgtaccg agggggagcg gccagtgacc
     4621 gataagatcg acgacccggt ctcgacggcc gacgccgacc aacaggtcgc ggcgggccgt
     4681 ccggacgagg ccctggattc gttgagacgc ctcctgtccc gacaggggct caagcctgcc
     4741 gaaaccgtcc tcctgaagcg caagatcgcc ggccttcttt ccacgcaggc caagtgggag
     4801 gaagcccgct cgctcctgat cgaggcgcgc gacctggcgg cccgctccgg cctcgcggcc
     4861 gaggaggcgc tgctgctcgt cgagttcggc aagcttcaca tggtgcgcgg cgagatcgat
     4921 cgcgcgctcg aatgcgcccg cggggcgatg gagcgcgccg aggcggcggg tgaaacactg
     4981 tcgaaggcgt atgccgagaa ccttaccggc tacgcgctct tcagccgcgg cctcctcgac
     5041 cagagccagg ccgccttcga gcgcgccctc atggcctgca aggcctcggg caacctccgg
     5101 gcactctcgc ttgtgtacaa caacctcgga ctgctctaca aggaacgctg ccagtggaag
     5161 aaggccctcg agcacttcca ggtggcgggc aacctgaacg ccatcgacgg cgccgagtcc
     5221 gagcgccgga aggggctgca gaacctgggc ctcgtccacc tgcgcctcgg ccagtggacc
     5281 gacgcgcggg cctgcttcga cgagagcctg gccatcggcc ggaagctcgg cgacccgctc
     5341 tccgtcatcc gcgcgctcct cggcctcgcg cagctgaacc tgtcggccgg ccaggccgac
     5401 catgcgaagg accagctcga gcagtcgctc gaactctgca ctcaggatgc ctacccgcgc
     5461 gagacggcgc tcacccatcg ttcgctgggc gtgatgcacc tgaaggcggg cgactacgag
     5521 cgcgcccgcc gctgcttcga gaccgcctgg aagcgggccg tcgagatcgc tcccgagggt
     5581 gacctcgcgg tcgagctcct cctgcaccgc gccgagctgt cgctggcggt cgggaaggcc
     5641 agggaagccc agagcctcgc gcagcgcagc cacgccctcg cgatgaaagc cggcgacgcg
     5701 ttctacgagg ccgaggcctt ccgcgtcctc ggcaacgtga tgtgggcgct cggcaacctc
     5761 gacgaggccg agaaccactt caaggaaggg atcaccgcct tccgttcgat cgacgcgccg
     5821 tacccgctgg cgctcgtcct cctcgacatg ggacgcctgg gcgccgagcg cctgacggcc
     5881 cgccaggagc ccgagaaggt cctcaacgcg ctggacgagg cgcttcgcct cttcacgcag
     5941 ctcggcgtgg gctcgctccg cggcgaaacg ctgctggccc tggcccgcgc gcatgtgcgg
     6001 tcggcggcct tcgaccgcgc ctcggcctgc ctggccgaag ccaccgaggc ctaccgcggc
     6061 ctcgacctgc cgaccgggat gagccgcgtg gccgaggtgc agaccgagct cgagggacgc
     6121 ttcgtcgacg agtcggtctc gcatctcaac gaattccgcg ccaccaaccg catcgaggag
     6181 atcctcgagg ctcgggccga cgccaccacc aagatcggcc agatcgtcga ggtggtgtcg
     6241 aacgcggtcg aagccgacgg catcctcctg atctccggac tcgggcagcg cgacgcagag
     6301 ccgctggcgt tccgcggcat gagcccgccg gtggcccgct cgatcggcga gcagctccac
     6361 gcgctccacg gcaccgccat cggcacgcgc ccccagatcg tcctgaacac cgaggcgtgc
     6421 acggtgcccg aactttccga cctggccgtg cggcaccagg cccagagcct catcctcatc
     6481 ccgatccgct gggatgacgg gcgcgaggcg ctcctctatc tcgatcgcac caggaccggc
     6541 cgtcgcgggc cgttccgcca gagcaacctg aacctgaccg tcgtgctctc ctcatacctg
     6601 cacgacctcc tggcccagat ggaccgggac gtgacgatca gcgaaaacct ccggatcaag
     6661 agccagctcg aggagaagat cgcgctggcc gacgtggtca cccagaaccg cgacatgctc
     6721 ggcatcctga agctggtgga aaagatcaac cggagcgacc tcaccgtcct gctccagggc
     6781 gaaaccggca ccggcaagac gctgctggcc aagtcgatcc acctgtcgag cccgcgcggc
     6841 aagggaccgt tcgtcacggt ggactgcgcc gcccttccgg acaacctcct cgagagcgag
     6901 cttttcggtt atgtgaaggg gtcgttcact ggcgccatga gcgacaagaa ggggttgttc
     6961 gaggaggccg acggcggcac catcttcctc gacgaaatcg gccgggccgg gctcaccgtg
     7021 cagcgtcggc tcctccacct gctcgacaag ggagaggtac ggccggtcgg cagcaacagc
     7081 taccgcaagc tcgatgtgcg ggtgatctgc gccacctcga gcaaggacct ccgccacgac
     7141 gtggcgcagg gggccttcat caaggatctc tattaccggc tgaacgacat cagcgtggtg
     7201 gtgccgtcgc tccgtgatcg cgtggaggac atcccgctcc tggccgacta cttcctctcg
     7261 acgttcaccg gcgagaccga ccgcgacgtg ccgggcttca cccgggcggc gctcaaccgg
     7321 ttgctccgct acaactggcc gggtaatgtc cgcgaactcg agaaggtggt ccggcgcgcg
     7381 gccatcctgt gcgacgaggg tgcgccgatc aacgtcgagc atctccccga ggacctgctg
     7441 gggactgccg tcgacgaacc ggccatcctg accgacggcg ccagcatcca cgacaccgtg
     7501 gaggagatgg agcgccggat ggtgctcaag gcgctcgagg agaacaactg gaacaagagc
     7561 cgggcagcgg tggccctcgg gttgtcgcgc aagggcttga agaacaagat cgcccggtac
     7621 gacctggacc gtaggatcgt cacccgccgc tagaatcccc tcgtttcttc ggcggcgagg
     7681 aggagtcatg ccgggtgtgg tgggggcgcc ggtcgtccgc tcgatcacgg gtcgcgccag
     7741 tcgctccgaa cgaaaccgcc cgattcccgc ccttcttcgc ctcctccttt tcggcgcctg
     7801 ttgcctcggt ggcgtccttc tcgtcggcgc gccgatggcg tgggccgatc cgccccttcc
     7861 ggacaattcg cccgccgtcc tcgcggcgcg ccgcgcgctg aaggcaaatc ccgagtcgaa
     7921 ggaacgctgg ctcgagctgg cccacgtctg ctggaacctc ggtacgatcg aagggcggcg
     7981 cgcggccgac gaggtcctcg aggacgcgcg ccggaagtgg ccccatgaca gcgaaatcct
     8041 gctcgactgg gccgacctga accgtgagag gcgcttcatc tcgcgctcgc gtggactcta
     8101 ccgccgcgcg ctgaaggagg agcccggatc cgagcgggcc ctgacggggc tatccgccct
     8161 tgccatgtac gactggcgcc gctacatccg ccccaagtcg ctcatcgagg ccgacctccg
     8221 cgccggccag ctcatggcgg tgagcccgga caacgcggac ggcatggcgc tcggcgcctt
     8281 cacccgcctg atgcgcggcg acaccaccga ggcgatggcc cgcaccgagg ccctgctggc
     8341 ccgtcatccc gacgatccgc gcgggcactt cctcaagctc acgctggatg tggacgcggg
     8401 gcgctgggat gacgccgccg agcatgccga gctggccctg gcgcgactca ccgacgcggc
     8461 cgagatcgag gcctatcgat cgctcgagaa catccactgg gggcaggagg atcggcggca
     8521 ggagctcccc gactcgttga aggtgcggtt cgagcggaat ttctggaaac gacgggaccc
     8581 cacgccggcc cccgtccgga ccgagcgacg gctcgagcac ctccgtcgcg tcttcatgtc
     8641 cgacgagctg tacggtattc cggagacggg gctgcggggg tgggacaccg atcccggcga
     8701 ggcggtcatc cggttcggca tgccgaaggc gcgcatgctg gggacggggg tccaggtgtc
     8761 gtactcgttc cgggccgacc gcctgaccac ggtccacaat atcgacggct acgactacga
     8821 cttcaacttc gacgacgaaa tgctgaacgg caactgggtg gagccgagcg gattcctgac
     8881 cccttcgctg atggacaagg tggcggcggt gaaggaaggg tggatgcccg atccgatcgg
     8941 cggcccggtg ctcgacatgg tgttcgatgc cgtccagttc cgggggaaaa atgggtggac
     9001 gagggtcgag gccatcgtgg ccgcgccggc cgtccccgac accgccacct cctgggaacg
     9061 gccgatcgcg ctctacaccg acgaatggga gctggtggcc cagcgcggcg gcaccctgtc
     9121 gcacgcggcg cgcgtgaagg accgccagcg accggatgcc gggtggctgg tcgatctcgt
     9181 gccgatcgaa gtcgacccgg ggatcgactc ggtcttcatc gggagcgagc tggtggccgg
     9241 cctgaagatg gggcacggcg agcgcttcga ccgcctcctc ctgcgccagt ttcacgacga
     9301 ctcgctggaa gtgtccgacc tgatgctcct cgaccggatc gaccccgacc tggtggaagg
     9361 gccgttcgcc cggcgcgatg gcggcaccgt gccgagcccg gacgccatct acgccatcgg
     9421 cgaatcggtg ccggtctact tcgaagccta tggcctgacg cccgacagcg acggtcgctg
     9481 ccgttatgcc gtcaccgtcg agatcaacga cctgaagacg ttcggcgaat gggagagcaa
     9541 gcggcgctcc gcgctgaccg ccaaccgccg cccccgacgc ccgcgctcga cttcccgctt
     9601 cgaggagtgg agcccgacgc ccgacatcga gcggatgatc gacctctcgg ttgggcgcct
     9661 cccggccggc gatttctggg tgaagatcgt ggtggaggac ctggttcgca gccgtcgtgc
     9721 gcagggggag ggagcgttcc gcatggtgcc ggcacccaag tcgaagcatg gcgacaagca
     9781 cgggaagacg ccatgaatcg actgtcgacg gggacggcct ggctcatcgg cacggcggca
     9841 ttgctggtgg tgggcgtggc gctcatcgtg gccggtaccc gtccaccgtc gccggcgcgc
     9901 gatcgaacga cggatcgcac cagtcccgcg acttccgcgg cgccgccgaa ctctgcaacg
     9961 cccgcgggtt ccgtcgccca cgagaccacc ccggtggcca cgccgacagg ctccctggtt
    10021 cgtgccgatt ccaccaacac ccctccggcc ttgctcgccg cgctcgcggg aacatcggga
    10081 ggggatccgg cgctgg
//
LOCUS       DASVOD010000187         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_637508, whole genome shotgun sequence.
ACCESSION   DASVOD010000187 DASVOD010000000
VERSION     DASVOD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637508"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF720_03930"
     CDS             <1..249
                     /locus_tag="VF720_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7878531.1"
                     /translation="DPRVFWHRGPTKPEEVAFNQLPAVLEREKNSLPEDELIVLIKID
                     REATYDTMVRITDELGMAEIDKFSLVPLEDADRKAVPL"
     gene            246..1181
                     /locus_tag="VF720_03935"
     CDS             246..1181
                     /locus_tag="VF720_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7878532.1"
                     /translation="MSSFVWAKFMMLAQLDILTVVAWSVMGLFAVATIVSYFNRLATP
                     SQTSGEGVLSWVPIPENLPTSGEVAPYGAPVLKSSYQNFMTRGVVGATLFHALLIGGG
                     VGIATLLTREPVAQQAVVVNYAELGAPPSLSQKQAPNQAVVGPTPPPPPSAAIPIPVP
                     DATAPKNTTILSQDELGDLGPMFEGGEGEGSIWGGGGGGGFGGGAVTVTEGGDPNPTD
                     FQPVEQLPELITIPEPDYPQLAREAGVEGVVKLLVLVGKDGKVKNVKIEKSIEMLNSS
                     AEAAARQASFKPALWQKKPVAVWVSIPIRFSLRQG"
     gene            1351..2259
                     /locus_tag="VF720_03940"
     CDS             1351..2259
                     /locus_tag="VF720_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7878533.1"
                     /translation="MSDFLWSRQALLAGASAIDVLVLVMSALVLFAGALAAWRAWTAS
                     PLPIRWWNPVPERLERADSAYGAAALKKVYPDALRNGVTSAGAIHIVLATLAIAWMAR
                     TPPPQPVDPGCTITIPRWEKPPMITVTQDGKPGGGRAPLVLPDPRNVIPSPIETHVTT
                     LDPEDFRISDFMSPDGSIFGKGTGLDGEEKDGGPNGTEFGRNALADPDPNDAVPVDQL
                     PELIWMAEPKYPEMARLAGLEGVVQLRLLVDTGGRVTKSRVDRSIAGLDEAALASGVT
                     ARFRPALWRGKPVKVWVALPIRFSLL"
BASE COUNT          387 a          865 c          781 g          365 t
ORIGIN      
        1 gacccgcggg tcttctggca ccgcggaccc accaagcccg aggaagtggc gttcaaccag
       61 ctgccggccg tcctcgagcg ggagaagaac tccctccccg aggacgagct catcgtgctg
      121 atcaagatcg accgtgaggc cacctacgac acgatggtgc ggatcaccga cgagctgggc
      181 atggccgaga tcgacaaatt cagcctcgtg cccctcgagg atgccgatcg gaaggcggtg
      241 ccgctgtgag cagtttcgtc tgggcaaagt tcatgatgct ggcccagctc gacatcctga
      301 ccgtcgtcgc ctggtcggtg atggggctct tcgcggtggc cacgatcgtc tcgtatttca
      361 accggctcgc cacgcccagt cagaccagcg gcgaaggcgt gctctcctgg gtgccgatcc
      421 cggagaatct gccgacgtcc ggcgaggttg ccccctatgg cgcgccggtc ctcaagagct
      481 cctaccagaa cttcatgacc cggggcgtgg tcggtgccac acttttccac gccctcctga
      541 tcggcggcgg cgtcggaatc gccaccctcc tgacacgcga gccggtggcg cagcaggcgg
      601 tggtcgtcaa ctacgccgag ctcggcgcgc ccccgtcgct cagccagaag caggccccga
      661 accaggccgt ggtgggcccc acgcctcccc cgcccccgtc ggcggccatc ccgatcccgg
      721 tacccgacgc aacggccccc aagaacacca ccatcctctc gcaggacgaa ctgggcgacc
      781 tgggcccgat gttcgaaggc ggcgaaggtg aaggaagcat ctggggcggt ggcggtggcg
      841 gtggcttcgg cggcggcgcc gtcacggtca ccgaaggggg cgatccaaat cccaccgact
      901 tccagccggt cgagcagctc cccgagctga tcacgatccc cgagcccgat tacccgcagc
      961 tggcccgcga ggccggcgtg gaaggcgtcg tgaagctcct cgtcctggtg ggcaaggacg
     1021 gcaaggtcaa gaacgtgaag atcgagaagt cgatcgagat gctgaactcc tcggccgagg
     1081 ccgccgcacg gcaggcctcg ttcaagccgg ccctctggca gaaaaagccg gtggccgtct
     1141 gggtgtcgat cccgattcgc ttcagcctcc ggcagggtta gtcgatatac tgatggcatc
     1201 gtcacccggc ggccgccggg tgacgatggg ctccactccc cctcgcacgg ccgtacaccc
     1261 cctcgcacca acccccccag ggactgacgc gcgcgtcgcg cggggccatc cggtccgatc
     1321 gcggcacgcg cccgagcggg aggtggtttc atgtccgatt tcctctggtc ccgccaggca
     1381 ctcctcgccg gcgcctccgc catcgacgtc ctggtgctcg tcatgagcgc actggtgctg
     1441 ttcgccggcg ccctggccgc gtggcgcgcg tggacggcct cgccccttcc gatccggtgg
     1501 tggaatccgg tgccggagcg gctggagcgc gcggacagcg cgtacggtgc cgccgcactg
     1561 aagaaggtgt accccgacgc gcttcgcaac ggcgtcacgt cggcgggcgc cattcatatc
     1621 gtgctggcga cattggccat cgcctggatg gcgcggacac cgccgccgca acctgtcgac
     1681 cccgggtgca ccatcacgat ccccaggtgg gagaagcccc ccatgatcac cgtcacccag
     1741 gacgggaaac ccggcggcgg cagggctccc ctcgttctcc cggatccgag aaacgtcatt
     1801 ccgtcgccga tcgagactca tgtcacgacg ctcgaccccg aagacttccg gatcagcgac
     1861 ttcatgtcgc cggacggatc gatcttcggc aaggggactg gtctcgacgg agaggagaag
     1921 gatggcggac cgaacggcac ggagttcggt cgcaacgcac tcgccgaccc cgacccgaac
     1981 gacgcggtcc cggtcgacca gctccccgaa ctgatctgga tggccgagcc gaagtacccg
     2041 gagatggccc gcctggccgg cctggaggga gtggtccagc tccggctcct ggtcgacacc
     2101 ggcgggcgcg tcacgaagtc gcgcgttgac cgctccatcg ccgggctcga cgaagcggcg
     2161 ctggcatccg gcgtcacggc gcgcttcagg ccggccctct ggcggggaaa gccggtcaag
     2221 gtctgggtgg ccctccccat ccgcttctct cttctctgac aacccggggt gcacaccccc
     2281 ggccgcgggc gccacttgtc cgcggccggg gccgggggct aatctgctgg gcgatgaccc
     2341 gccccatcta cctcgacaac cacgccacca cgccgatcga tcccgtcgtc ctcgaggc
//
LOCUS       DASVOD010000188         2516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_429519, whole genome shotgun sequence.
ACCESSION   DASVOD010000188 DASVOD010000000
VERSION     DASVOD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429519"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..552)
                     /gene="ssrA"
                     /locus_tag="VF720_03945"
     tmRNA           complement(203..552)
                     /gene="ssrA"
                     /locus_tag="VF720_03945"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(598..673)
                     /locus_tag="VF720_03950"
     tRNA            complement(598..673)
                     /locus_tag="VF720_03950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(638..640),aa:Lys,seq:ctt)
     gene            complement(1013..2497)
                     /locus_tag="VF720_03955"
     CDS             complement(1013..2497)
                     /locus_tag="VF720_03955"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX7878534.1"
                     /translation="MNHWIDNKPYDGPVARWGEVTDPALGKVTHRVAFADEALVNQAV
                     AAAVRASATWRVSSLSARTRIFFAFRELLNARKREVAELLTREHGKVLSDAMGEVQRG
                     LEVVDFACGLPQLLKGEFSENVSSEVDSYSIRQPLGVVAGITPFNFPAMVPMWMYPLA
                     IACGNTFILKPSEKDPSASVYCAKLFAEAGLPDGVLNVVHGDKVSVDAILHHPDIAAV
                     SFVGSTPVARYIYEQGTKAGKRVQALGGAKNHMVVLPDADLDLAADSAVNAGYGSTGQ
                     RCMAISVIVAVGDVGDRLIPKIRERIKKLNVAPGFAENAEMGPLTTRDAWDRTRSYVE
                     QGVASGATLLEDGRDVKPVGGENGFFVGPCLFDGVKPDMSIYKDEIFGPVLSVVRADS
                     YEEAMNLISSGPYANGVAIFTNDGGAARRFQNEVPVGMVGINVPIPVPMAYYSFGGWK
                     QSLFGDAHVHGKEGVHFYTRGKVVTARWPDPSHGGVNLGFPQMK"
BASE COUNT          438 a          808 c          852 g          418 t
ORIGIN      
        1 caggccggcg aggaccctcg cggttccgtg agagaacctg ttccggaaca ggtagctcgg
       61 atctacccag gccggcgagg accctcgcgg ttccgtgaga gaacctgttc cggaacaggt
      121 agctcgggtg ctgagcggaa agtggggcgg cgtgggagga actggcggga gggtcgtccg
      181 aagcggagct tcgtccgact gatggtggag gcggcgggac tcgaacccgc gtccgaagtg
      241 acatccccga aaggtactac gcgcgtgtcc gaccgatttt agctctcgcc gctccgaccg
      301 ccgatcggcg ggctgttcgg attggccagc ctctgaatct cgccggtgtc cgcgaggcac
      361 ggaaccagcc agtccctctg atggcgtttg accctggccc aagggaccga gaccaggcaa
      421 acgtcgctac tgtttaggca gcgagtgcga actcagtgtt cgcagttaca cttgtccacc
      481 tttttaacga ggccggtggg ccctcggcgc gcaccttccg cttcagttac cccgtcgaaa
      541 ccaatcgccc ccacatgaag cttacggcac cttctttgga tgcccatggc gggaaaatgg
      601 tgcgccgcga tggaatcgaa ccatcaacct actgattaag agtcagttgc tctgccaatt
      661 gagctagcgg cgcgtcggga tccggcggtg tcatccaccg ggccgaaaca gcgacgcagg
      721 acgttatcag agagtttccg gggggtcaac cggaaggtgg cgactgcgag gaaactgttt
      781 cggaatcagg cggaagcgat tcaaaacgag acgatcgggg agccgcttgg gaccggcggg
      841 gaccgggagg cgtccgtggc tgttgcctct gccgcgtgtc ttacattgta agacatcgcg
      901 cgaaattctt gacgcgcacc ccccgagcgc tgtccgagag caccccgagc gcccgtgacc
      961 ccgagcgacc gcgccgagcg tccgcaaccc gagcgcccgc agcgccgggc gcctacttca
     1021 tctgaggaaa gccgaggttc acgccaccgt ggctgggatc aggccagcgc gccgtgacga
     1081 ccttgccgcg cgtgtagaag tgcacccctt ccttgccgtg cacgtgggca tcgccgaaga
     1141 ggctctgctt ccagccgccg aacgagtagt aggccatcgg caccgggatc ggcacgttga
     1201 tgccgaccat gccgaccggc acctcgttct ggaagcggcg ggcggcgccg ccgtcgttgg
     1261 tgaaaatggc cacgccgttg gcgtaggggc ccgacgagat gaggttcatg gcctcttcat
     1321 agctgtcggc gcggacgacc gacaggaccg ggccgaagat ctcgtccttg tagatcgaca
     1381 tgtcgggctt cacgccgtca aagaggcagg ggccgacgaa gaagccgttc tcgccgccga
     1441 ccggtttcac gtcgcggccg tcctcgagca gtgtggcgcc ggaggccacc ccctgctcca
     1501 cgtaggagcg ggtacgatcc catgcatcgc gcgtggtgag cgggcccatc tcggcgttct
     1561 cggcgaaacc gggggccacg ttgagcttct tgatccgctc gcggatcttc gggatgaggc
     1621 gatcgccgac atcccccacc gccacgatga ccgagatggc catgcagcgc tggccggtcg
     1681 atccgtagcc ggcgttcacg gccgagtcgg cagccaggtc caggtcggcg tccggcagga
     1741 ccaccatgtg gttcttggcg ccgccgaggg cctgcacgcg cttcccggcc ttcgttccct
     1801 gttcatagat atagcgggcc accggcgtcg aaccgacgaa gctgacggcg gcgatgtccg
     1861 ggtgatgcag gatggcatcg accgacacct tgtcgccgtg gaccacgttg agcacgccgt
     1921 cgggcaggcc ggcctcggcg aagagcttcg cgcagtagac cgacgccgac ggatcctttt
     1981 ccgagggctt caggatgaac gtgttgccgc aggcgatggc cagcgggtac atccacatgg
     2041 gcaccatcgc cgggaagttg aacggcgtga taccggccac cacgccgagg ggctggcgga
     2101 tggaatagct gtcgacctcg ctcgacacgt tctcgctgaa ttcacccttg agcagctgcg
     2161 gcaggccgca ggcgaagtcg accacctcga ggccgcgctg cacctccccc atcgcgtcgg
     2221 agagcacctt gccatgttcg cgcgtgagga gctcggccac ctcgcgcttc cgtgcattga
     2281 ggagctcgcg gaaggcgaag aagatgcgcg tgcgggcgga gagcgaggaa acgcgccacg
     2341 tggcggacgc gcgaaccgcc gcggccaccg cctggttcac cagcgcctcg tcggcgaagg
     2401 ccacgcggtg cgtgaccttg ccgagcgcgg ggtcggtcac ctcgccccag cgggccaccg
     2461 ggccgtcgta cggcttgttg tcgatccagt ggttgatcgt gccgagctcg gacgtg
//
LOCUS       DASVOD010000189         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_272941, whole genome shotgun sequence.
ACCESSION   DASVOD010000189 DASVOD010000000
VERSION     DASVOD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272941"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VF720_03960"
     CDS             <1..607
                     /locus_tag="VF720_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878535.1"
                     /translation="IARLDPVDGALFLAAHAVRHLDAPEGAYRSLVDLAWFLAAEPAL
                     DAETLASRAIAQRLDRVMAAALGWLDEQVPGTGGVAAVTLRGRVIPRREQDEAVRDLR
                     AMMTDLARRPAHRPLAVASGITGCWQRSGRARWAFLFRGLAPAPGDMARDLAPEQRRR
                     ALPLRYLALVGNYWSEALRLGPGGFVMAARLGRLRRRLSQS"
     gene            complement(558..2504)
                     /locus_tag="VF720_03965"
     CDS             complement(558..2504)
                     /locus_tag="VF720_03965"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878536.1"
                     /translation="MRRTRGKQRGTTEPAGETAVRTALTIAVLAGYFTGLLGPSNWVW
                     GMNLLRYYPSAFGLLLMSAAVALVWFLPPIDDDSRSSLAFARVPPVGAGVGVALIGLA
                     LFVTFRTKSFLLGDGLELIRRLHQGEVPAPRSPLFNAVQPVFYRMLEQGDSARGGESA
                     AAISFLAGFLGLLGAVFYLARLARRQPAAAITAGTLLLLTCGMQLFFGYVEVYSLLAA
                     ATLVFMAAAFDRLTGGGRTALIGATLAFGAAMLSHPFGTTLFLPWLYLVAGSGNDKSW
                     HINWRLLAILLTSVIAAVLVLTLAFALNPAWQEPGMRFRYLSPQVQASGLIRGLLQID
                     SKLSWASEYTVLSWRHVADCVNSVWLPGAAALAFVVGCLAAPATRSAMRRPTVVMPLL
                     GFVMILVYRIIWRTPLGAMRDWDLYAGLGFGIAAIAAGLSLAGAGRRLAGPVVAASLF
                     FLVPWIGIQIDSSRAARRHFDGVDAEPRPEPMVAAQFHGVMGDRFSSMREFGMAAKAY
                     ERALAMRPRHEYAWRLGMAQFAAHDYEHAVINLKRALELRPGDVTTRITLGEVCIDAG
                     RMDEADGWLDPVLAEDPASAGAWLQKGRAEQRRGRFDAAARAYARADTLFAPNARARR
                     DHDRARESLRTATVGGGAAPTGPP"
     gene            2621..>3258
                     /locus_tag="VF720_03970"
     CDS             2621..>3258
                     /locus_tag="VF720_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878537.1"
                     /translation="MSDNQPLVPMTGGDRRFVAICLLVFLAGVAVVAVLFHRAFPEAS
                     IEFKVDRGEARSIAESFLAERGLPVTGDRFAGIFEADDDTKVYLERTLGLEEANRVYK
                     EPVKLWRWQLRWFRPLRKAEVRVAVTPEGEVVGFRREIEESAPGAHLESSEARGLAES
                     FLASRPGLDPAGLEFLETSATERPARRDHSFTWERRGWSAGAATIRVQVDVFG"
BASE COUNT          508 a         1128 c         1166 g          456 t
ORIGIN      
        1 catcgcacgg ctcgaccccg tcgacggcgc gctcttcctc gccgcgcacg cggtccggca
       61 tctcgacgcg ccggaggggg cctaccgaag cctggtcgac ctggcctggt tcctcgcggc
      121 ggaaccggcg ctcgatgcgg aaaccctggc atcgcgcgcc atcgcgcagc gtctcgaccg
      181 ggtgatggcc gccgctctcg gctggctgga cgagcaggtg cccgggaccg gaggcgtcgc
      241 cgcggtcacg ctccgcggga gggtcattcc gcgacgcgaa caggacgagg ccgtccgcga
      301 tctgcgcgcg atgatgaccg acctggcgcg ccgccccgcc catcgtccgc tggcagtcgc
      361 ttcggggatc acggggtgct ggcagcgatc cggccgtgcc cgctgggctt tcctcttccg
      421 ggggctcgcg cctgcccccg gcgacatggc gcgcgatctc gcgccggagc agcggcggcg
      481 ggcgctcccg ctgcgctacc tggccctggt cggcaactac tggagcgagg cgcttcggct
      541 cggcccgggt ggtttcgtca tggcggcccg gttggggcgg ctccgccgcc gactgtcgca
      601 gtcctgagcg actcgcgcgc gcgatcgtgg tcgcgccggg cccgggcgtt cggcgcgaaa
      661 agagtgtccg cccgcgcgta ggcacgagcg gccgcgtcga agcggccgcg gcgctgctcg
      721 gccctcccct tctgcagcca ggctccggcc gatgccggat cctcggccag aaccggatcg
      781 agccagccgt cggcctcgtc catccgcccg gcgtcgatgc acacctctcc cagggtgatg
      841 cgggtggtca cgtcacccgg gcggagttcg agtgcgcgct tcagattgat caccgcatgc
      901 tcgtagtcgt gggcggcgaa ctgggccatc ccgagccgcc aggcgtattc gtgtcgcggg
      961 cgcatcgcga gcgcgcgttc gtaggccttg gccgccattc cgaactcacg catgctggaa
     1021 aagcggtcgc ccatgacgcc gtggaactgt gcggccacca tcggttcggg acgcggctcg
     1081 gcgtcgacgc cgtcgaaatg gcgacgggcc gcccggctgg agtcgatctg gatgccgatc
     1141 cacggaacga ggaaaaagag cgaagccgcc acaacgggcc cggcgagacg acgccccgca
     1201 ccagccagcg agagaccggc cgcgatggcc gcaatgccga accccaggcc ggcgtagagg
     1261 tcccagtcgc gcatggcgcc gagcggcgtg cgccagatga tccggtagac caggatcatc
     1321 acgaagccga gcagcggcat caccacggtg ggacgacgca tggctgatcg cgtggccggc
     1381 gcggcaagac acccgaccac gaaggccagg gccgcggcgc cggggagcca gaccgagttg
     1441 acgcagtcgg cgacgtggcg ccacgacagc accgtgtact cgctggccca cgagagcttg
     1501 ctgtcgatct gcaggagtcc gcggatgagc ccgcttgcct gcacttgcgg cgagaggtat
     1561 cggaaccgca tccccggttc ctgccaggcg ggattgagtg caaaggcgag ggtcaacacg
     1621 aggacggctg cgatcacgct cgtcagcagg atcgccagaa gccgccagtt gatatgccac
     1681 gacttgtcgt tgccactacc ggccacgagg tagagccagg ggaggaagag cgtcgttccg
     1741 aaggggtgcg acagcatggc cgcgccgaag gccagggtgg cgccgatcag cgcggtccgc
     1801 cctccgccgg tcaggcggtc gaacgccgcg gccatgaaga cgagcgtggc ggcggccagc
     1861 agcgagtaga cctccacgta gccgaagaag agctgcatgc cgcaggtgag gaggaggaga
     1921 gtccccgcgg ttatcgcggc ggcgggctga cgccgggcga gccgtgccag gtagaagacg
     1981 gctcccagga ggcccaggaa accggccagg aagctgattg cggcggccga ttcccccccc
     2041 cgggcggaat ccccctgctc gagcattcga taaaagaccg gctggacggc attgaagagg
     2101 ggggatcgcg gggcggggac ctccccctgg tggagacgcc gaatcagttc gagcccgtcc
     2161 cccagcagga agctcttcgt ccggaacgtg acgaagaggg cgaggccgat cagggcgacg
     2221 ccgacgcccg cccccaccgg tggcacccgc gcgaaggcca gggagctgcg actgtcgtcg
     2281 tcgatcggcg ggagaaacca gaccagggcc accgccgcgc tcatgagcag gagcccgaat
     2341 gccgatggat agtagcgaag caggttcatg ccccagaccc agttcgacgg cccgaggagg
     2401 cccgtgaaat acccggcgag gaccgcgatg gtcagcgcgg tacggacggc ggtttccccc
     2461 gccggttctg tcgtgcctcg ctgttttccc ctcgtccgtc gcatggctcg tccgggttgg
     2521 ttatcatgcc gccgcgcggc cgggcgtgga gattagtgag cgtgccgccg ccgaacaagg
     2581 ggatcgtgac acggacagcg ttccgcccag ccggccaacg gtgagcgaca accagccact
     2641 cgtcccgatg acggggggtg atcgccgttt cgtggcgatc tgcctcctcg tcttcctcgc
     2701 cggcgtcgcc gtcgtggcgg tgctcttcca ccgcgccttc cccgaagcct ccatcgaatt
     2761 caaggtcgat cgcggcgaag cccgctcgat cgccgagtcg ttcctcgccg agcgtggcct
     2821 cccggtaacc ggcgatcgat tcgccggcat cttcgaagcc gacgacgaca ccaaggtgta
     2881 cctcgagcgc acgctcggcc tcgaggaggc caaccgcgtc tacaaggagc cggtcaagct
     2941 ctggcgctgg cagctccgct ggttccggcc gcttcgcaag gccgaggtgc gggtggccgt
     3001 cacgccggag ggagaagtgg tgggctttcg ccgcgagatc gaggagtcgg cgccgggggc
     3061 gcacctcgaa tcgtccgagg ccaggggcct cgccgaatcc ttcctggcct cacgtccggg
     3121 cctcgacccg gcgggcctcg aattcctgga gacgtcggcc accgagcgtc cggcccgccg
     3181 cgatcactcc ttcacctggg agcgccgcgg ctggagcgcc ggcgccgcca cgatccgcgt
     3241 gcaggtggac gtgttcgg
//
LOCUS       DASVOD010000190         6414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_324731, whole genome shotgun sequence.
ACCESSION   DASVOD010000190 DASVOD010000000
VERSION     DASVOD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324731"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="VF720_03975"
     CDS             complement(<1..737)
                     /locus_tag="VF720_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7878538.1"
                     /translation="MTRPGRNLLFLVVLVLAGGLLSCQSESDFAPIPIDSSGTTLLAG
                     TVTTATGPLANAIVTLEPLVGELPARVHAAAGGSEALTLPNAGSEPLSPLGEGARFDT
                     PAIRATVTDALGRFEFAGVHTGGFQVEARGRDHLGATRRVTIGPVAALAETTLIDIRL
                     TPTGDYYGTVTLETLSSHQGTLVYIDGTSHVAATRPSGAYVIEGVPVGTHTVRATHPG
                     WLDRTAMGTIAAAGDSIPLATLVLPRDS"
     gene            923..1531
                     /locus_tag="VF720_03980"
     CDS             923..1531
                     /locus_tag="VF720_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7878539.1"
                     /translation="MKLRAILLLLAVIALITKAGNVRAGTLCGTVRDGITNSPVPGAG
                     VFVFHPGGAFAGQAAVTDMAGAFCIAAIPPGTYNVQVRVNDYLEKVIAGVVVTGDVSG
                     VDVELGARVFLAAPHPNPARGQVFLGFELAVPAALVTLEVFDVAGRLVQGWRSDDMGA
                     GVHQVEWSFDDRNGTRVAPGCYFVRLSAAGVSVTRRVVYVGP"
     gene            1777..2430
                     /locus_tag="VF720_03985"
     CDS             1777..2430
                     /locus_tag="VF720_03985"
                     /inference="COORDINATES: protein motif:HMM:NF016270.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEX7878540.1"
                     /translation="MNGSDMHHSHQGTRETIGSLFARGFYILMPLLLILLIGKKLIEM
                     LQGMFSPVLDRLPGEIIRSATVRFLLLCLLVAAILVLVGFIARRRVGRAIGAWLERRI
                     LNKLPFYTHLRGLTTTLASTDHDHSLRTARIEVDIPGLEQLGVIMEYHEDGSATVFLP
                     SAPNPSSGTVVIVARERIRELTIPVRSVVGCMGRYGHGTAAVLKGADYDPMTSGPRK"
     gene            complement(2397..3962)
                     /locus_tag="VF720_03990"
     CDS             complement(2397..3962)
                     /locus_tag="VF720_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEX7878541.1"
                     /translation="MSTTVEAAPTDDATTLTPASFREALTPAELAFEQRRRRFGLGLA
                     PLVFLLLWLLPFPGLTPEAHRFAAILAFTVILWVTEAVPLPVSGILGVALSVILGVEP
                     AREALAPFADHLVFLLIGGFIMAEAIFVHGLNRRFAFGVLSLPWVGARPARVLVAYAA
                     VTAFISMWVSNTASTALMLPIGLSLLEFLYTRRGAASPLPDRNYATGLMLVTAFAASL
                     GGLGTPIGTPTNLIGMGFLEEQIHRRVSFFEWVLLALPILVILMAVTLVNLNRSCPAG
                     LEEVHGGRRFILTEKGRLGPLSGGERNVLAVFALAIFLWTLPGFVGLAGFEGLSTKLS
                     GRFPEGIVALLCASLLFFLPTDLVHRKPTLQWNQAARIDWGTVLLLGSGIALGRMAER
                     TGLSREIGQAMAAHIPQGSLLVMLLVSTLAAVLISETTSNMASVTMLVPLVISVSQGA
                     GVDPVLPALGATMGGSLGFMLPVSTPPNALVYGSGYVPIARMVKHGLVLDVAGVIVVV
                     GVLYFLGPLVIGS"
     gene            complement(3959..4993)
                     /locus_tag="VF720_03995"
     CDS             complement(3959..4993)
                     /locus_tag="VF720_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7878542.1"
                     /translation="MKAAQFAEHGDAEVLRLVDIADPVAGPDEVVVALEAAALNRLDL
                     AIRKGLPGIKVARPHTLGCEGYGRVASVGAGVGHVAPGDTVVVTPGMSCLRCEACARG
                     EDSLCRSYRMIGYQRPGCYAAQVVVPAGNVFHADDRLTPHEWAALPLVYLTAWRMLMA
                     RARLAPGETVLVQAAGSGVGIAAIQIAKLMSARVITTVGSEAKRTKALALGADHVIDY
                     TRNDVATEVKHLTGGAMVDIVIEHTGAATWEGSVGSLKWGGRLVTCGATDATPVSVDL
                     RHLFVKQFDWLGSYMGGRHDLMQVLKLAGAGRLQPVVDRVIPLAEAAEAHRYLDSRAA
                     FGKVVLSMNG"
     gene            complement(4990..5439)
                     /locus_tag="VF720_04000"
     CDS             complement(4990..5439)
                     /locus_tag="VF720_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HEX7878543.1"
                     /translation="MIPYEALPTVNATLNALSAGFLVLGWRFIRLGDRGSHRWCMLAA
                     TSVSVLFLVSYIVYHLKAGTTRFPDVGVVRAIYLGILGTHTLLAAIVPVMVLLTLAPA
                     LSKRFDRHRRIARYTLPIWLYVSVTGVVIYLMLYHLAPHLTGTGRTS"
     gene            complement(5436..6068)
                     /locus_tag="VF720_04005"
     CDS             complement(5436..6068)
                     /locus_tag="VF720_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7878544.1"
                     /translation="MRRIWWTLLVVVLGGVIAIWLAGELIPGFPTNSARSGTLPSFGP
                     APSFELVDQLGRPFSSTALAGRPWVADFVFTRCAGPCPLMTRRMAALVDTLGPRTPVK
                     FVSFTVDPEYDTPAVLREYAGGFRADPDRWRFLTGLPADLHQVSVNGFHLAMGEAEAD
                     SATQLISVAHSTRFILVDAAGQVRGYYDGEDAAAMTALARDAASLARRGR"
     gene            complement(6094..>6414)
                     /locus_tag="VF720_04010"
     CDS             complement(6094..>6414)
                     /locus_tag="VF720_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878545.1"
                     /translation="GFLVAWSMESTQGFHAVMNLFLLPLWLLSGSAFPIDGARGWLRG
                     VMMVNPVTYGTALVRRLVDPVSAATSPGLPEIGISWIVTVVFAVVMVVAASLITMRRR
                     NVAA"
BASE COUNT         1117 a         2126 c         2284 g          887 t
ORIGIN      
        1 gaatcgcgcg gcaggacgag cgtggccagc gggatgctgt cgccggcggc cgcgatggtg
       61 cccatggcgg tgcggtcgag ccagccggga tgcgtggcgc gcaccgtgtg cgtcccgacc
      121 gggacccctt cgatcacata ggcgcccgag ggcctggtgg cggccacgtg cgaggtgccg
      181 tcgatgtaga cgagcgtccc ctggtggctc gacagcgttt cgagcgtgac cgtgccgtag
      241 tagtcgccgg tgggagtgag gcggatgtcg atgagcgtgg tttccgcgag cgcggccacc
      301 ggaccgatcg tgacacggcg cgtggcgccc aggtggtcgc ggccgcgggc ctcgacctgg
      361 aagccgccgg tatggacgcc ggcgaattcg aagcggccga gggcgtcggt gaccgtggcg
      421 cgaatggcgg gtgtgtcgaa cctcgcaccc tcacccaggg gagacaacgg ttccgagccg
      481 gcattcggca aagtcagcgc ttccgagccg ccggccgccg catgcacgcg ggccggaagc
      541 tcgccgacca gcggctcgag cgtgacgatc gcgttggcca gcggccccgt ggcggtggtg
      601 accgtgccgg ccaggagggt cgttcccgag ctgtcgatgg ggatcggtgc gaagtcggat
      661 tcggactgac acgagaggag cccaccggcc agcacgagca cgacaaggaa aagcaggttg
      721 cggccgggcc gggtcatcgt ccatccccct gaaacgtgac aatccggaag ccccgaaacc
      781 cggctgcacg gccgactttc gggctcaaat tggcttgagg tcagcgcaga atgtccgata
      841 gtatgacaaa attggattcc gaatctgaag acaaatcgcc cttcccccca cgatcacgcg
      901 ccatcgccca cctgagggtt gcatgaagct tcgagctatc ctgttgctgc tggcggtgat
      961 cgcactgata acgaaggcgg ggaacgtccg ggcaggcacg ctctgcggca cggtgcggga
     1021 cggcatcacg aacagcccgg tgccgggcgc gggggtcttc gtcttccatc ccggcggggc
     1081 tttcgccggg caggccgcgg tcaccgatat ggccggcgcc ttttgcatcg ccgccatccc
     1141 gcccggcacg tacaacgtcc aggtaagggt caacgactac ctggagaagg tgatcgccgg
     1201 cgttgtcgtg acgggggatg tctccggcgt ggacgtggag ctcggtgcgc gggtcttcct
     1261 cgcggcgccc catccgaatc ccgcgcgggg gcaggtcttc cttggtttcg agctcgcggt
     1321 tcccgcggcc ctggtcacgc tcgaagtctt cgacgtggcc ggacgcctcg tgcagggttg
     1381 gcgttccgac gacatgggcg caggcgtgca ccaggtggaa tggagcttcg acgaccgcaa
     1441 cggaacccgg gtggcgcccg gatgctactt cgttcggctg agcgcggccg gcgtgagtgt
     1501 gacgcggcgc gtggtctacg tcggaccgta gagaccggcc ggtgtgaacc agcggatcgt
     1561 gctgcatggc cggcccgact gccaaggttc ccaggtgcac gaggtcatgg acgacgtctt
     1621 ctcgggcgag gaagagagac gcccaccaca ccaccggcac gcccctcgcg cgcgtgactg
     1681 attgaccgac gtggtggcgg cccgcggatc ggacgggctt atactgtcgc gcgtgcacga
     1741 tgaacaacac cctttcggaa ggaccgggcg gacacgatga acggttccga catgcaccac
     1801 tcacaccagg gaacccgcga gacgatcggc tcgctgttcg cgcgcggctt ctacatcctg
     1861 atgccgctcc tgctgatcct cctgatcggc aagaagctga tcgagatgct gcagggaatg
     1921 ttcagcccgg tcctcgatcg gctccccggt gaaatcattc gcagcgccac ggtccgcttc
     1981 ctcctcctgt gcctgctcgt ggccgccatc ctggttctcg tcggattcat tgcgcgtcga
     2041 cgcgtggggc gggcgatcgg tgcctggctc gaacgaagga tcctgaacaa gcttcccttc
     2101 tacacgcacc tgcgcggcct gaccaccacg ctggcttcca cggaccacga tcactccctc
     2161 aggacggcgc gcatcgaggt ggacattccg gggctcgagc agctcggcgt catcatggaa
     2221 tatcacgagg acgggagcgc caccgtattc ctgccgtcgg cgcccaaccc gagcagcggg
     2281 acggtcgtca tcgtggcgcg cgagcggatc cgcgaattga ccatccccgt tcgcagcgtg
     2341 gtcggctgca tgggacggta cgggcatggc acggcggcag tgctgaaggg ggcggactac
     2401 gacccgatga ccagcggccc aaggaagtag agcaccccca ccaccacgat cacccccgcc
     2461 acatcgagca cgagcccgtg cttcaccatc cgggcgatcg gcacgtagcc cgatccgtag
     2521 accagtgcgt tggggggcgt ggaaaccggc agcatgaaac cgaggctccc gcccatggtg
     2581 gcgccgagtg ccggcaggac gggatcgacg cccgccccct ggctcaccga gatgaccagc
     2641 ggcaccagca tcgtgaccga cgccatgttg gaagtggttt cgctgatgag gaccgcggcc
     2701 agcgtgctga ccagcagcat cacgagaagc gatccctggg ggatgtgggc ggccatggcc
     2761 tggccgatct cgcgcgagag gccggtgcgc tcggccatgc gcccgagcgc gatgccgctc
     2821 ccgaggagga gaacggtgcc ccagtcgatc cgtgccgcct ggttccactg cagcgtgggc
     2881 ttccggtgga cgagatcggt ggggaggaag aagagcaacg acgcgcagag gagcgccacg
     2941 attccctcgg gaaaacgacc ggaaagcttc gtggagaggc cttcgaaacc ggccaggccc
     3001 acgaaaccgg ggagcgtcca gaggaagatg gccagcgcga agacggccag cacgttccgt
     3061 tcgccgcccg agagggggcc gaggcgcccc ttctcggtga gaatgaagcg gcgcccgccg
     3121 tgcacctcct cgaggccggc cgggcaggag cggttcaggt tcaccagcgt caccgccatc
     3181 aggatgacga ggatcggcag cgccaggagg acccactcga agaagctcac ccgccggtgg
     3241 atctgctctt ccaggaaacc catcccgata agattggtgg gggtgccgat cggcgtcccg
     3301 aggccgccga gcgaggcggc gaaggcggtg accagcatga ggccggtggc gtagttccgg
     3361 tcggggagtg ggctggcggc gccccgccgc gtgtagagaa actcgaggag cgagaggccg
     3421 atcggcagca tgagagcggt gctggcggtg ttggacaccc acatggaaat gaaggccgtc
     3481 accgccgcgt aggccaccag caccctcgcg gggcgcgcgc cgacccacgg gagcgagagc
     3541 acaccgaacg cgaaacgccg gttgagccca tgcacgaaga tcgcctcggc catgatgaag
     3601 ccgccgatca acaggaagac caggtggtcg gcgaacggcg ccagcgcctc gcgggccggc
     3661 tccacgccga ggatgaccga caacgccacg cccaggatcc ccgacacggg caacggcacg
     3721 gcctcggtga cccagaggat gaccgtgaaa gccaggatgg ccgcgaaacg atgcgcctcc
     3781 ggcgtcaggc cgggaaacgg caggagccag aggagcagga agacgagcgg cgcgaggccg
     3841 aggccgaacc gccgccgccg ctgctcgaac gccagctcgg ccggcgtgag tgcctcgcgg
     3901 aacgaggcgg gggtgagcgt ggtggcgtcg tcggtcgggg cggcttccac cgttgtgctc
     3961 atccgttcat tgacaggacg actttgccga aggccgcgcg gctgtcgaga tagcgatgcg
     4021 cctcggcggc ctcggccagc ggaataacgc gatcgacgac cggctggagc cttcccgcgc
     4081 cggccagctt gagcacctgc atcaggtcgt ggcgtcctcc catgtaggaa ccgagccagt
     4141 cgaactgttt gacgaacagg tgccggagat cgacggacac cggcgtggcg tcggtggcgc
     4201 cgcaggtgac gaggcggccc ccccacttga ggctgccgac gctcccctcc caggtggcgg
     4261 cgcccgtgtg ctcgatgacg atgtcgacca tggcgccacc ggtcaggtgc ttcacttcgg
     4321 tggccacgtc gttccgcgtg taatcgatca cgtggtcggc gccgagcgcc agcgccttcg
     4381 tgcgcttggc ttccgagccg accgtggtga tgacgcgggc gctcatcagt ttcgctatct
     4441 ggatggcagc gatgccgacg ccggagccgg ccgcctgcac cagcaccgtt tcgccgggcg
     4501 cgaggcgcgc gcgggccatc agcatccgcc acgcggtaag gtagacgagc ggcagcgccg
     4561 cccactcgtg cggcgtgagg cgatcgtcgg cgtggaacac gttgccggcc ggcacgacca
     4621 cctgcgccgc gtaacagccg gggcgctggt aaccgatcat ccggtagctg cggcagaggc
     4681 tgtcctcgcc tcgggcgcag gcctcgcagc ggaggcacga catgccgggc gtcaccacca
     4741 cggtatcgcc gggggccacg tgcccaacgc ccgcgcccac cgaggccacc cgtccgtacc
     4801 cttcgcagcc gagcgtgtgg gggcgggcca ccttgatgcc ggggagcccc ttccggatgg
     4861 ccaggtcgag ccggttgagc gcggcggcct cgagcgccac caccacctcg tccgggccgg
     4921 caaccgggtc ggcaatgtcg acgagccgga gtacctcggc atcgccatgc tcggcgaatt
     4981 gggcggcctt cacgacgtcc tccccgttcc ggtgagatgc ggcgccagat gatagagcat
     5041 caggtagatg acgacaccgg tcacgctcac gtagagccag atcggcagcg tgtaccgggc
     5101 gatcctccgg tggcgatcga accgcttcga caaggcgggc gccagcgtga ggagcaccat
     5161 cacggggacg atggcggcca gcagggtgtg cgtgccgagg atgccgaggt agatcgcgcg
     5221 cacgacgccg acatcgggga agcgcgtggt gccggccttc aggtgataca cgatgtacga
     5281 gacgaggaac aggaccgaca cggaggtggc cgccagcatg caccagcgat ggctcccgcg
     5341 atcacccagc cggatgaagc gccagccgag gaccaggaag ccggcgctca gcgcgttgag
     5401 cgtggcgttc accgtcggca aggcctcgta ggggatcatc ggcctcgacg cgccagcgag
     5461 gcggcatcgc gggccagggc cgtcatggcc gcggcgtcct cgccgtcgta gtagccgcgg
     5521 acctggccgg ccgcatccac caggatgaag cgggtgctgt gggccacgct gatgagctgc
     5581 gtggcggaat cggcctcggc ctcgcccatc gccaggtgga agccgttcac cgacacctgg
     5641 tggagatcgg ccgggaggcc ggtcaggaag cgccagcggt ccgggtcggc gcggaagccg
     5701 ccggcgtact cgcgtagcac cgccggcgtg tcgtattccg gatcgaccgt gaacgacacg
     5761 aacttgaccg gcgtgcgcgg cccgagcgta tcgacgaggg ccgccatccg ccgcgtcatc
     5821 aaggggcacg ggccggcgca ccgggtgaag acgaagtcgg ccacccacgg cctgccggcc
     5881 agcgcggtgg aggaaaaggg gcgcccgagc tggtcgacca gctcgaacga gggcgcgggg
     5941 ccgaacgacg ggagcgtgcc cgaccgcgcg gagttggtgg ggaagccggg gatcagttca
     6001 cccgccagcc agatggcgat gacgcccccc agcaccacca cgagcagcgt ccaccagatc
     6061 cggcgcatca ggccgccagc acgtcgaggc gcatcaggcc gccacgtttc gtcggcgcat
     6121 cgtgatcagc gaggccgcca ccaccatcac gaccgcgaac accacggtca cgatccacga
     6181 gatgccgatt tccggaagtc cgggcgacgt ggccgccgag accggatcga ccagccgccg
     6241 cacgagtgcc gtgccgtagg tgaccgggtt caccatcatc acgccccgca gccagccgcg
     6301 cgccccgtcg atcgggaagg ccgagcccga caggagccag agcggcagga ggaagaggtt
     6361 catcacggcg tggaagccct gcgtcgattc catcgaccag gccaccagga agcc
//
LOCUS       DASVOD010000191         4566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_220955, whole genome shotgun sequence.
ACCESSION   DASVOD010000191 DASVOD010000000
VERSION     DASVOD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220955"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="VF720_04015"
     CDS             <1..149
                     /locus_tag="VF720_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006260635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HEX7878546.1"
                     /translation="EEIMTAFHTIHEQGNTVILVTHEPDIAAHAQRVIRVRDGRIESD
                     EKTR"
     gene            154..1374
                     /locus_tag="VF720_04020"
     CDS             154..1374
                     /locus_tag="VF720_04020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878547.1"
                     /translation="MDLWLESAKLALQSLKVNKLRTFLTVLGNIIGVMFVIAILSITQ
                     GMTAYVSEKVVDQGSNKFWVERVGMVRSWDEWVEKNKRKEFTMRDLDALRERGTTFGM
                     VGAARDRSARIKYRNRSINGITVIGVTDDHPEAEKYTLEAGRHLAGPDIEHRRFVAII
                     GHRVKNELFPGLDPIGRDIRVGPNWFTVIGICEKQGNVLGEDRDNFVAVPLTAYESMW
                     GTEDDLQFSIQARNASVYEQSQDEATQILRSQRRVPPGKPDDFDLLTAEMFMQIYNSF
                     TSGAYIVMVALAGIALVVGGIVIMNIMLVSVTERTREIGIRKAIGAKRGDILRQFVVE
                     AMILGIMGGLMGVALGVGLAKLVEALSPLPAVIQPWVIALGLSVATGVGLFFGIWPAM
                     KAAKLDPIAALRYE"
     gene            1374..2633
                     /locus_tag="VF720_04025"
     CDS             1374..2633
                     /locus_tag="VF720_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878548.1"
                     /translation="MAGRPTRAQRMANRFGNIREAVAMALDTIKVSKMRSFLTILGVF
                     IGVSSVIAMASCIEGLNRSMAKQISSLGSETIRIRRFPPFHGGDLPDSLRLRKYFTLN
                     DVDGIKRTCPAVGAVSILMLDGDRVKYRGTTTGMTDIYGADVAFPEIQNVTVERGRFY
                     NDGDIERNMRVCVIGADHVESLFAGIDPIGQWVTVKGHKFEVIGVFAHRGKFLGQSMD
                     DMFVIPYTTFERMIGGRRMVIDARPASAALLPTAIDQITDSLRRSRGVPPEKPNDFEV
                     FTSDQLMNIYKQITGAFYIVMFAISSIALMVGGIGVMNIMLVAVTERTREIGIRKAIG
                     AKRSDILWQFLIEAMTLTGLGGILGILFGAGIGQLVRMLTPLPSYVPPMAAIAGFVFS
                     VGIGLFFGIWPAAKAARLHPVDALRYE"
     gene            complement(2647..3000)
                     /locus_tag="VF720_04030"
     CDS             complement(2647..3000)
                     /locus_tag="VF720_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878549.1"
                     /translation="MNRIRHLLRLAALAALLAPLGCDYASIQLHFSGALVVYVYDDNL
                     AIRGTIIEVVETGEKATTNASGLATFTLSPGTYTVRAYDINRGGPCCAYVEEAVEVER
                     DGEARVAIWTCVRCR"
     gene            3199..>4566
                     /locus_tag="VF720_04035"
     CDS             3199..>4566
                     /locus_tag="VF720_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7878550.1"
                     /translation="MLGYLVVGATLASPSRQAFAGLWVFSSPASGSDVQSVADRAADG
                     DTLLFMPGRHEIQLELTDKSIVLVGQGGPGQVTLTRTAEEVIFKPGMIRITGATGGGN
                     SFRASGIRFEGGFSHGRAQAAAIWAWEVSTVVIEDCQFINNRAVPSSMAGSNFGGAVR
                     VWGASTIRLQGCLFKGNSSEASFHHDSSGGAAQLSGQFIEVRHCEFEANRAEGYFCGG
                     GGGALIALGNFVTIADCNFFRNSASDGAALGANSNITIERCSFQENPGGGSLFGPCGV
                     QEGSSVNISGVASIYQSTFIDNRSNAPALRLLGAGPHHVETSAFVGNGVSGGGSPAVR
                     LGGSAAANVTFARNLVAFNHGPGIAHAITTEGAASCNIIWANNPDFIQGSDWRGAHDN
                     QASDPLFCEGAVNDLLVANNSPCLPGGSGHPESCGVIGSVTAGCAVVSIRPSSWSGIK
                     LRFGST"
BASE COUNT          818 a         1443 c         1459 g          846 t
ORIGIN      
        1 gcgaagagat catgacggcc ttccacacga tccacgaaca ggggaacacg gtcatcctgg
       61 tgacgcacga gcccgacatc gccgcgcacg cccagcgggt catccgggtc cgtgacgggc
      121 gtatcgaatc ggatgaaaag acgaggtaac gacatggacc tctggctcga aagcgccaag
      181 ctcgccctcc agtcgctgaa ggtcaacaag ctccgcacct tccttaccgt cctcggcaac
      241 atcatcggcg tcatgttcgt cattgccatc ctctccatca cgcaggggat gacggcctac
      301 gtctccgaga aggtcgtcga ccaggggtcg aacaagttct gggtggagcg ggtcggcatg
      361 gtccgctcgt gggacgagtg ggtcgaaaag aacaagcgca aggaattcac catgcgcgac
      421 ctcgacgcgc tgcgcgagcg tggcaccacc ttcggcatgg tcggggcggc gcgggaccgc
      481 tcggcgcgca tcaagtaccg caatcgctcc atcaacggca tcacggtcat cggcgtcacc
      541 gacgaccacc ccgaggccga gaagtacacg ctcgaggcgg gccgtcacct ggccgggccc
      601 gacatcgaac atcgccgctt cgtggccatc atcggccacc gcgtcaagaa cgagctcttt
      661 cccggcctcg acccgatcgg ccgggacatc cgggtggggc cgaactggtt cacggtgatc
      721 ggcatctgcg agaagcaggg gaacgtgctg ggggaggatc gggacaactt cgtggccgtc
      781 cccctcaccg cctacgaatc gatgtggggc accgaggacg acctccagtt ctccatccag
      841 gctcgcaacg cctccgtgta cgagcagtcg caggacgagg ccacgcagat cctccgctcc
      901 cagcgccgcg tgcctcccgg caagccggac gacttcgacc tgttgaccgc cgagatgttc
      961 atgcagatct acaacagctt cacctccggc gcctacatcg tgatggtggc cctggccggc
     1021 atcgcgctgg tggtcggcgg catcgtcatc atgaacatca tgctggtgtc ggttaccgag
     1081 cggacgcgtg agatcggcat ccgcaaggcc atcggcgcga aaaggggcga catcctccgc
     1141 cagttcgtgg tggaggccat gatcctcggc atcatgggcg gcctcatggg cgtggcgctg
     1201 ggcgtgggcc tggccaagct ggtcgaggca ctctcgccgc tgccggcggt gatccagccg
     1261 tgggtcatcg ccctcgggct ttcggtggcc accggcgtgg ggctcttctt cggcatctgg
     1321 ccggccatga aggccgccaa gctcgacccg atcgccgcgc tgaggtacga gtgatggccg
     1381 gccggcccac ccgcgcccag cgcatggcca acaggttcgg aaatatccgc gaggccgtgg
     1441 ccatggcgct cgacaccatc aaggtgagca agatgcgctc gttcctgacc atcctgggcg
     1501 tcttcatcgg cgtctccagc gtgatcgcga tggccagctg catcgagggg ctcaaccgct
     1561 cgatggccaa gcagatctcg agcctcggct ccgaaacgat ccgcatccgc cgcttcccgc
     1621 cgttccacgg cggtgatctc cccgattcgc tccgcctacg gaaatatttc accctgaacg
     1681 acgtggacgg catcaagcgc acgtgccccg ccgtcggcgc cgtctcgatc ctcatgctgg
     1741 atggcgaccg ggtgaagtac cgcggcacca ccaccggcat gaccgacatc tacggcgccg
     1801 acgtggcctt ccccgagatc cagaacgtca ccgtggaacg gggccgcttc tacaacgacg
     1861 gcgacatcga gcggaacatg cgcgtctgcg tgatcggcgc cgaccatgtc gaatctctgt
     1921 tcgccggaat cgacccgata ggccagtggg tgaccgtcaa ggggcacaag ttcgaggtca
     1981 tcggcgtctt tgctcaccgc ggcaagttcc tcgggcaatc catggacgac atgttcgtga
     2041 ttccgtacac cacgttcgag cggatgatcg gcggccgccg catggtcatc gacgcccgcc
     2101 cggcctcggc cgcgctcctg ccgaccgcca tcgaccagat caccgacagc ctgaggcgct
     2161 cccgtggggt tccgccggag aagcccaacg acttcgaggt cttcacctcc gaccagctca
     2221 tgaacatcta caagcagatc accggcgcct tctacatcgt gatgttcgcc atcagcagca
     2281 tcgccctcat ggtcggcggc atcggcgtca tgaacatcat gctcgtggcc gtcaccgaac
     2341 gcacccgcga aatcggcatc cgcaaggcca tcggcgccaa gcgctccgac atcctctggc
     2401 agttcctcat cgaggccatg acgctgaccg gcctcggggg gatcctgggg atcctgttcg
     2461 gggcggggat cgggcagctg gtgcgcatgc tcacgccact gccgtcctac gtgccgccta
     2521 tggcggcgat tgccgggttc gtcttctcgg tggggatcgg tttgttcttc gggatctggc
     2581 ctgcggccaa ggcggcccga ctgcatccgg tggatgcgtt gaggtatgag taggggtcag
     2641 gccctctcat cggcatcgca cgcacgtcca gatagccacc cgcgcctcac cgtcccgctc
     2701 cacctcaacg gcctcctcca cgtacgcgca acacggcccg ccccggttga tgtcgtacgc
     2761 gcgcacggta tacgtaccgg gcgagagggt gaacgtggcc aggccggagg cgttggtcgt
     2821 ggccttctcg cccgtctcca cgacttcgat gatggtcccc ctgatcgcga ggttgtcgtc
     2881 gtacacgtag acgaccagcg cccccgagaa gtggagctgt atggaggcgt agtcgcagcc
     2941 gagaggtgcg aggagggcgg cgagggccgc gagccggaga aggtgacgga tgcggttcat
     3001 gggtggcgtc tcgggatagc ggagcaaggt ggacgcgaag gtagatatcg tccagccgac
     3061 tctctcatta tacttgaggt caagacgtag gcccgagcac tatcctgatg ttcctcctcg
     3121 atactgcagt gggcgttgag tccaccagat gccggtccga ccaaccttga gaggggcaga
     3181 ttgcgaaccg cagccgcatt gctcggctac ctggtcgtgg gagcgaccct tgcgagccct
     3241 tcgcgccagg ccttcgcagg actttgggtg ttcagctcgc cggcctccgg ctccgatgtg
     3301 caatcggtcg ccgatagagc ggcggacggt gacacgctgc ttttcatgcc tggccgacac
     3361 gaaattcaac tggagctcac cgacaaaagt atcgtgttag ttggccaagg tgggcctggg
     3421 caggtcacgc tgacgcggac ggcggaggaa gtcatcttca agcccggaat gatccgaatc
     3481 acgggggcga cgggtggcgg caatagtttc cgcgccagcg gaatccgatt cgagggagga
     3541 ttcagtcacg gtcgtgccca ggctgctgcg atctgggcgt gggaggtctc gacagttgtc
     3601 attgaagact gccaattcat caacaaccgc gccgtgccgt cgagtatggc cggcagcaat
     3661 ttcggcgggg ccgtcagggt gtggggcgcc agcacgattc gacttcaagg ttgtcttttc
     3721 aagggcaatt cgagcgaggc aagtttccac catgatagtt ccggcggagc ggcgcagttg
     3781 tcgggtcagt tcattgaggt tcgccattgc gaatttgagg ctaaccgggc ggaaggctac
     3841 ttctgtggcg gcggtggcgg tgcactgatc gcattgggga actttgtgac gatcgccgac
     3901 tgcaatttct ttcggaacag tgccagcgat ggggcagcgc tcggcgccaa ttcgaatatt
     3961 acaatcgagc gctgcagttt ccaggagaat cccgggggcg gatcgctgtt tggtccttgc
     4021 ggcgtccagg aaggttccag cgtcaatatt tccggcgtcg ccagcatcta tcagtcgact
     4081 ttcatcgata accggagtaa cgccccggca ctccggttgc tcggggccgg accccatcac
     4141 gtcgagactt ccgctttcgt aggtaacggc gtgtcgggcg gtggatcccc cgcggttcga
     4201 ttgggtggga gcgcggccgc aaacgtcaca tttgcccgaa atctggttgc cttcaaccac
     4261 ggcccgggga tcgcacacgc gattaccacg gagggtgcgg catcgtgcaa catcatttgg
     4321 gccaacaacc cggacttcat ccagggatcc gattggcggg gtgcgcatga caatcaggct
     4381 agtgatccgc ttttctgcga aggggcggtc aacgatctgc tggttgcgaa caattccccc
     4441 tgtctgcctg gtggcagtgg gcatcccgag tcgtgcggtg ttatcggaag cgtgacggct
     4501 gggtgcgccg ttgtctcgat tcgtccctcc agttggagtg ggatcaagct tcgattcggt
     4561 tccacc
//
LOCUS       DASVOD010000192         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_638048, whole genome shotgun sequence.
ACCESSION   DASVOD010000192 DASVOD010000000
VERSION     DASVOD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638048"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF720_04040"
     CDS             <1..366
                     /locus_tag="VF720_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878551.1"
                     /translation="HAWRVRTGDEPIAPIPGTNTWLLVWTSDEGAALEASVHRHLGLA
                     TVESIRRGWLWQFGAAPGVDAAVLLHAAREAAFGDGGSHGFLVHDAWQEGRLYADRPS
                     VYDCREAFGGAGVSTGRRG"
     gene            404..1825
                     /gene="purF"
                     /locus_tag="VF720_04045"
     CDS             404..1825
                     /gene="purF"
                     /locus_tag="VF720_04045"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX7878552.1"
                     /translation="MSFTPRDRDDDRFHDACGVFGAVGVDAAAHAVYLGLYSLQHRGQ
                     ESTGIVAADGGEFQLHKAMGLVADVFDNVRLDSLKGRMAIGHNRYSTAGDSTLRNAQP
                     ILVTYHGGHLAVAHNGNIVNAIDLKKEMEGGGHIFQTTSDSEVILHLIARSPARSFEE
                     ALGPALSRLSGAFSLVLMTDTALYAVRDPLGFRPLSIARLRDGWVVASETCAFDINEA
                     EYVRDVEPGEVVRIDESGPTTILRLPSKRQASCIFELIYFARPDSTVFGESVNRVRRE
                     LGRKLAEEHPADADIVIAVPDSSNAAALGYAEKSGLPFEFGLIRNHYIGRTFISPRQF
                     TRDHGVRVKFNPVKGVLDGKRVVVVDDSIVRGTTSRKLVKLIRRAGAKEVHFRISSPP
                     IVSPCFFGIDTPRRAELIGANHTVEEIGTYLRVDSIGYLSQAGMLSVVKNAAGHCTAC
                     FDENYPVPLAGLPTDRALEPVGR"
     gene            1833..3344
                     /gene="lysS"
                     /locus_tag="VF720_04050"
     CDS             1833..3344
                     /gene="lysS"
                     /locus_tag="VF720_04050"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEX7878553.1"
                     /translation="MTNELIRVRRQKVDDWRALGVEPWAYAYAVSHKAADIHADADAM
                     VNASVPVNVTVAGRLVGKRGHGKTCFAHLLDVSGKIQLYFKRDDLGDLFDRVEMLDVG
                     DVIGVAGFIFRTRTGETTVHVTSFELLAKSLRPLPEKWHGLKDLETRFRQRYVDLIVH
                     EDVREIFRKRARFISAMRRWLDDHGFLEVDTPTLQPIYGGAFATPFVTHHEALGIPFY
                     LRISNELYLKRLIVGGLERVYEIARDFRNEGIDRTHNPEFSLLEFYQAYADYNVIREE
                     AEELLSYAFEAALERKELTYGEHQISLARPWAVGTYFGLLQEATGRNLIDADEAALRE
                     IGREHHVDLGGAKGYGKVVDTLFSDLVQPRLIQPTFVLDYPFAISPLAKRKRGEPRLV
                     ERFELFIAGMEVANCFSEQNDPLEQEAQFDLQADFRESGDLEAQVKDTDYLRALQYGM
                     PPTGGIGFGVDRLVMLLTDSHNIRDVLLFPALRPETEEELDATDTPESAESGN"
     gene            3449..>4195
                     /locus_tag="VF720_04055"
     CDS             3449..>4195
                     /locus_tag="VF720_04055"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7878554.1"
                     /translation="MRQFTVSLGLVSMVGMLCLWPAGTGANVPTLEPGFRAFDPGVST
                     CELVVADLDNDDRADLVAASWQHPVILWAGDNGLYSPQALTTGAANRSVAVADFDEDG
                     TPDLAVANGGAESVDIWLGTGSGFTFLGFLDGVDGAYSIRSADFDADGHADLAVTNGL
                     SGGFLAVGLGNGDGTFSKAALHPAGNAPTDLEIADLNADGAADIVVANADAGIPSVSV
                     YIGAGDGSFLPSPQLVTGPPCFALVVADFDG"
BASE COUNT          691 a         1434 c         1336 g          734 t
ORIGIN      
        1 catgcctggc gcgtgcggac cggtgacgaa ccgatcgccc ccatcccggg taccaacacc
       61 tggctcctcg tctggacgtc ggacgagggt gccgcgctcg aggcctccgt gcaccggcat
      121 cttggccttg ccaccgtcga atcaatccgg cgcggctggc tctggcagtt cggagctgcg
      181 cccggagtgg acgcggcagt attgctccac gccgcccgcg aggcggcttt cggcgacggc
      241 ggttcgcacg gcttcctggt gcatgacgcc tggcaggagg ggcgtctgta cgccgatcgg
      301 ccgagcgttt acgattgtcg cgaagcgttc ggcggtgccg gtgtttcgac cggtcgccgc
      361 ggctgaacgc cgccgacccc gcgtcacgaa aggacttcaa tccgtgtcat tcaccccccg
      421 ggaccgggac gacgatcgtt tccacgacgc ctgcggcgtc ttcggcgcgg tgggcgtgga
      481 cgccgccgcc cacgcggtct acctcggcct ctattcgctc cagcaccggg gccaggaatc
      541 gaccggcatc gtggcggccg acggcggcga attccagctg cacaaggcca tgggcctggt
      601 ggccgacgtc ttcgacaacg tccgtctcga ctccctcaag gggcggatgg ccatcggcca
      661 caaccgctac tccacggcgg gcgattccac gctccggaac gcccagccca tcctggtcac
      721 gtaccacggc gggcacctgg cggtggcgca caacggcaac atcgtcaacg ccatcgacct
      781 gaagaaggag atggagggtg gcggccacat cttccagacc acctccgatt ccgaggtcat
      841 ccttcacctc atcgcccgct cgccggcgcg atcgttcgag gaggccctcg ggccggccct
      901 ctcgcgcctt tcgggcgcct tcagcctggt gctgatgacc gacaccgccc tctatgccgt
      961 gcgcgacccg ctcggcttcc gacccctgtc gatcgcgcgc ctgcgcgacg gctgggtggt
     1021 ggcctccgag acgtgcgcct tcgacatcaa cgaggccgag tacgtgcggg acgtggagcc
     1081 gggcgaggtg gtccgcatcg acgagagcgg ccccaccacc atcctccggc tccccagcaa
     1141 gcgccaggcc tcctgcatct tcgagctcat ctatttcgcg aggcccgatt ccacggtgtt
     1201 tggcgaaagc gtgaaccgtg tgcgccgcga gctcggccgc aagctggccg aggagcatcc
     1261 ggccgatgcc gacatcgtga tcgcggtgcc cgattcgagc aatgcggcag cgctgggata
     1321 tgccgaaaag tcgggccttc ctttcgaatt cggcctcatc cgaaaccact acatcggccg
     1381 cacattcatc agcccccgcc agttcacgcg cgaccatggc gtgcgcgtca agttcaatcc
     1441 cgtgaagggg gtcctcgacg gcaagcgcgt ggtggtggtc gacgactcga tcgtccgcgg
     1501 caccaccagc cggaagctcg tcaagctgat ccgccgggcc ggcgcgaagg aagtacactt
     1561 ccgcatctcc tcgccgccga tcgtctcccc ctgcttcttc ggcatcgaca cgccccgccg
     1621 ggcggaactc atcggcgcca accacaccgt tgaggagatc ggcacctacc tccgtgtgga
     1681 ttccatcggc tatctctcgc aggcggggat gctcagtgtc gtgaagaacg ccgccggcca
     1741 ctgcaccgcc tgcttcgacg agaactaccc ggtgcccctg gccgggctcc ccacggatcg
     1801 cgcgctcgag ccggtggggc gctgaccggc cgatgaccaa cgaactcatc cgcgtccgcc
     1861 gccagaaagt cgacgactgg cgcgccctcg gcgtcgagcc ctgggcgtac gcctacgccg
     1921 tgagccacaa ggccgccgac atccacgcgg atgccgacgc catggtcaac gccagcgtgc
     1981 cggtcaacgt caccgtggcc gggcgcctgg tcggcaagcg cgggcacggc aagacctgct
     2041 tcgcgcacct gctcgacgtg tcgggaaaga tccagctcta cttcaagcgc gatgacctgg
     2101 gcgacctctt cgaccgggtc gagatgctcg acgtggggga cgtgatcggc gtggccggct
     2161 tcatcttccg cacccggacg ggggagacca ccgtccacgt cacgagcttc gagctcctgg
     2221 cgaagagcct gcgcccgctt cccgagaagt ggcatggact gaaagacctg gaaacccgct
     2281 tccgccagcg ctatgtcgac ctcatcgtcc acgaagatgt gcgcgagatc ttccggaagc
     2341 gggcgcgctt catcagcgcc atgcgccgct ggctcgacga ccacggtttc ctcgaggtcg
     2401 atacccccac gctccagccg atctacggcg gcgccttcgc cacgccgttc gtcacgcacc
     2461 acgaggcgct cgggatcccg ttctacctgc ggatctccaa tgagctctat ttgaagcggc
     2521 ttatcgttgg cgggttggag cgcgtgtacg agatcgcgcg cgacttccgc aacgagggga
     2581 tcgaccgcac gcacaacccc gagttcagcc tcctcgagtt ctaccaggcg tacgcggact
     2641 acaacgtcat ccgcgaggag gccgaagagc tgctcagcta cgccttcgag gcggcgctcg
     2701 aacgcaagga gctcacctac ggtgagcatc agatttcatt ggctcgaccc tgggccgtcg
     2761 ggacctactt cgggctgctc caggaagcca ccggccggaa cctgatcgat gccgacgagg
     2821 ccgcgctccg ggagatcggc cgggagcatc acgtcgacct gggcggtgcc aaggggtacg
     2881 gaaaggtcgt cgatacgctc ttttccgatc ttgtccagcc caggctgatc cagccgacat
     2941 tcgtgctgga ctatccgttt gccatatcgc cactcgccaa gcgcaagcgc ggcgagccgc
     3001 gcctcgtgga gcgcttcgag ctcttcatcg ccggcatgga agtggccaac tgcttcagcg
     3061 aacagaacga tccgctcgag caggaagccc agttcgacct ccaggccgac ttccgcgaaa
     3121 gcggggacct cgaggcccag gtgaaggaca ccgattacct gcgggccctc cagtacggaa
     3181 tgcctcccac cggcggcatc ggctttgggg tcgaccggct cgtcatgctc ctgacggaca
     3241 gccacaacat ccgcgacgtg ctgctgtttc cggcgttgag gccggaaacg gaggaagagc
     3301 ttgatgccac cgacacgccg gagtccgctg aatccggaaa ttgatgccct ggcccgcacg
     3361 atggcagggc ccgcgtcgtc cgtcgggatt cgctggtaga atcgtggcgc cctgccagtt
     3421 tggacaacga ttcgatcggg agctccacat gcggcaattc accgtcagct tgggccttgt
     3481 gagcatggtg ggaatgcttt gcctgtggcc ggccgggact ggagcaaatg tcccgactct
     3541 cgagcccggt tttcgggcct tcgatcccgg tgtgtcgacc tgcgaactgg tagttgccga
     3601 tctggacaat gatgatcggg ccgatcttgt cgcagccagt tggcagcatc ctgtcattct
     3661 ctgggcgggc gacaacggtt tgtattcgcc acaggcgctg acgacgggcg cagcgaatcg
     3721 atcggtcgct gtagcggact tcgacgagga cggaacacct gacctcgcgg tcgccaatgg
     3781 cggagccgag tccgtcgaca tctggttggg caccggcagc gggttcactt tcctgggctt
     3841 ccttgacggg gtcgatggcg cctattcgat ccgctcagca gactttgacg cggacggtca
     3901 tgccgacctt gcggttacga acggtctctc cggaggtttc ctcgcggtcg gtctggggaa
     3961 cggtgacggg acgttttcga aggccgcgct tcatcccgcc ggcaatgccc ccacggacct
     4021 ggagatcgcc gacctcaatg ccgatggggc agccgacatc gtggttgcca acgccgacgc
     4081 gggaattccg tcggtgtcgg tctacatcgg cgcaggggat ggatcgtttc tgccgtcgcc
     4141 ccagctggtg acgggcccac cgtgcttcgc gctggttgtt gcggacttcg atggc
//
LOCUS       DASVOD010000193         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_273332, whole genome shotgun sequence.
ACCESSION   DASVOD010000193 DASVOD010000000
VERSION     DASVOD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273332"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="VF720_04060"
     CDS             complement(<1..>1517)
                     /locus_tag="VF720_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX7878555.1"
                     /translation="DRRRIQEVANERAVQLFPAANEAAVALVEVAGRFAEPNLIYERT
                     ETMHLEADARNAVATISGRVESGERIIDSHTKITPEEYEKLRSLEVATNRLSRQPGTS
                     GWFMPLVGRALLVFMLVAIFAVFLKRHRPRLYDDLTTLTFLTSVSILVLLVAALVIRF
                     APEASPYLIPVGLASILITLLLDDQLAGAASVAMAILIGIVGGLGLPFVIVSIVGSLA
                     ASYAVAGIRHRWEFYRAMIFMSLAYAVTILAVELITSSSPFQVAMKDSLWGVINAVSA
                     TTAAIVLLPVFERGFGLCTDITLIELADLNRPIFKRMMLEANGTYHHTMVIGSLSEAA
                     ATAVGANPLLARVGAYYHDIGKVPKAEYFGENLKPGMKNPHEKLTPTMSCLILESHVR
                     EGQEMARELKLPKRVADFIPEHQGTTLMSYFYHKALEMDPGVEERDYRYPGPKPQSKE
                     TALVMLADSVEATARSLADPSPSRIKAVVKRIIDQRSADGQLDKCNLSLQELARA"
BASE COUNT          249 a          484 c          520 g          264 t
ORIGIN      
        1 gcgcgcgcgc cagctcttga agtgacaggt tgcacttgtc gagctgcccg tcggcggagc
       61 gctggtcgat gatccgcttc acgacggcct tgatccggct ggggcttgga tcggccagcg
      121 agcgcgcggt ggcttcgacc gagtcggcca gcatcacgag cgcggtttcc ttcgactgcg
      181 gcttcggccc ggggtaacgg tagtcacgct cctccacgcc ggggtccatc tcgagcgcct
      241 tgtgatagaa ataactcatc agcgtcgtcc cctggtgctc ggggatgaaa tcggcaaccc
      301 gcttcggcag cttcagctcg cgggccatct cctgtccttc gcgcacgtgg ctctccagaa
      361 tcaggcacga catggtcggc gtgagtttct cgtgcggatt cttcatgccg ggcttgagat
      421 tttcgccgaa gtactcggcc ttgggcacct tgccgatgtc gtggtagtag gcgcccacgc
      481 gcgccaggag aggattggcg ccgaccgccg tggccgccgc ttccgagagg cttccgatca
      541 ccatcgtgtg gtgataggtg ccgttggcct cgagcatcat ccgcttgaag atcggccggt
      601 tgaggtcggc caattcgatc agcgtgatgt cggtgcagag cccgaatccc cgctcgaaca
      661 ccggcaagag gacgatggcc gccgtggtgg ccgacactgc gttgatgacg ccccacagcg
      721 agtccttcat ggccacctgg aagggggagc tcgaggtgat cagttccacc gccaggatcg
      781 tcaccgcgta ggccagcgac atgaagatca tggccctgta gaactcccag cggtgccgga
      841 tgccggccac cgcgtagctc gccgccaggc tcccgacgat cgacacgatg acgaacggaa
      901 ggccgagtcc ccccacgatg ccgatcagga tggccatggc cacgctggcc gcgccggcca
      961 gctggtcgtc gagcagcagc gtgatcagga tcgaggccag ccccaccggg atgagatacg
     1021 gcgacgcctc cggggcgaac cggatgacga gcgccgccac cagcaggacg aggatgctga
     1081 cggaggtcag gaaggtcagc gtcgtcaggt cgtcgtacag gcgcgggcga tggcgcttga
     1141 ggaagaccgc gaagatggcc acgagcatga agacgaggag cgcgcggccg accagtggca
     1201 tgaaccaacc gctggtgccc ggctggcgcg ataaacggtt ggtggccacc tcgagcgagc
     1261 ggagtttctc gtattcctcg ggggtgatct tggtgtggct gtcgatgatc cgctcgccgc
     1321 tctccacccg tcccgagatg gtggccacgg cgttccgggc gtcggcctcg aggtgcatcg
     1381 tctcggtgcg ctcgtagatc aggttcggct cggcgaagcg cccggccacc tccaccaggg
     1441 ccaccgcggc ctcgttggcg gccgggaaga gctgcaccgc ccgttcgttg gccacttcct
     1501 ggatgcggcg gcggtcg
//
LOCUS       DASVOD010000194         4407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_742424, whole genome shotgun sequence.
ACCESSION   DASVOD010000194 DASVOD010000000
VERSION     DASVOD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742424"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VF720_04065"
     CDS             <1..544
                     /locus_tag="VF720_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX7878556.1"
                     /translation="GEMLELEKKRDLTTSEADYFRMIDEKTATLIAAACEIGGYLTYG
                     DGKERQALHRFGTELGMAFQITDDLLDFVGDSGHIGKSTGADIRDGKITLPLIHALRQ
                     APPEKANAIGEYIRATDITEPAWNAIFDFTAEFGGLDYGRAMAQKYADRAVRALDEFD
                     DSPIKSTLATAVDYVVHRRR"
     gene            522..926
                     /gene="rsfS"
                     /locus_tag="VF720_04070"
     CDS             522..926
                     /gene="rsfS"
                     /locus_tag="VF720_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX7878557.1"
                     /translation="MSSTAGAERERPRRTGKASADSHALALAAAREALKKKAQDVVVL
                     DLRTLTGVCDFFVIVTGTSDVQVRAIADQVEEGLREQGHDVWHVEGRTAGKWVLLDFV
                     DVVVHVFHHETRELYQLERLWRDAAREEMADE"
     gene            919..2604
                     /gene="argS"
                     /locus_tag="VF720_04075"
     CDS             919..2604
                     /gene="argS"
                     /locus_tag="VF720_04075"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX7878558.1"
                     /translation="MSSAPLATIEQQLAARINDTIGRLWPESAHLVPNPEVAVPREAG
                     HGDYSTNAAMLLAKALKKNPREFAGALAEELKKSDDLLDGVEVAGPGFINLTLSRTVL
                     NDTLKRVLAEGSAYGCSNAGLGERLQVEFVSANPTGPLNVVNARAAAFGGTLARLFNA
                     AGWKCDTEFYVNDVGTQIDLLGQSLLARYRESKGGGPAVIPENGYQGLYLADIAGELK
                     GPDVDAALAKPNDTAIAADFSKIATARIRAWQKGDLARYGTEFDRWYLQSELYPEAVQ
                     KTLAFIKERGYVVEKDGAQWLATTRFGDDQDRVLVRQTGEPTYFLADIAYHVDKHNRG
                     YAHVIDIWGPDHHGHIPRMQAGMEILGYGRDWLEVIIVQQVNLLSAGQPVKMSKRAGE
                     LVTLSDLVDDVGKDTAIFFFLMRRASSHLDFDLDVARKTSDENPVYYVKYAHARIASL
                     LRKAVESSQPGANDPAAGESSLLTHSTELDLMRLITRLPEVIVGGARHREPHRLTNYL
                     REVASAFHPFYHQCRVVGEEPKLAHARLLLCAAVKQVLANGMALINVDAPESM"
     gene            2609..3535
                     /locus_tag="VF720_04080"
     CDS             2609..3535
                     /locus_tag="VF720_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX7878559.1"
                     /translation="MSDQEAEVPGPEIVAVPPPPPWEPITPVPASKPFPGWWASIGLL
                     VAIQFLANLVGFIFLAPGIVSDVMAHGTLTRTLPLAAVGIVANSIAFGLVTWFAWRAS
                     KLTAAEAFPLRRVSGGAWFTAALLMGGTVVLAEFVSSGMMRLWPPPDFVRDLFLQLVG
                     DDAPVVVSIIFLVIVAPITEEFFFRGVLQKGLTRRYGRKAGVIGSGLLFGLIHLIPWQ
                     VVPAALLGLVFAWWTDRTGSLWPALVAHAVNNGMSYYLSRSQSGRDVLEPGPIDPVAL
                     AIGAGLLLAGLALSPRLLRPTEPETVGERSFT"
     gene            3532..>4407
                     /locus_tag="VF720_04085"
     CDS             3532..>4407
                     /locus_tag="VF720_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7878560.1"
                     /translation="MSALVVGSVALDTVITPFGRADEALGGSASFFSVAARLFTPVNL
                     VAVVGEDFPKAHVELLESRGVNTDGLVTAPGRTFRWAGEYSFDLNSRTTLDTQLNVFA
                     DFDPVVPEAYRNPRFLFLANIDPGLQRRVLDQVNRPEMVLMDTMNFWIERSRESLMET
                     LRRVDLVFLNDGETRELTGESNLIKAARAVLDMGPSVVVVKKGEHGSLLFTRDFTFSA
                     PAYPIEHLFDPTGAGDTFAGGFVGHLARSPRVDESELRRAVVTGTVMASFSVESFSLD
                     RLADVTMDEVAGRVRE"
BASE COUNT          731 a         1534 c         1420 g          722 t
ORIGIN      
        1 cggggagatg ctcgagctgg agaaaaagcg tgacctgacc accagcgagg ccgactactt
       61 ccggatgatc gacgagaaga ccgccaccct gatcgccgcc gcctgcgaga tcggcggcta
      121 cctcacgtac ggagacggca aggagcgcca ggcgctccac cgcttcggca ccgagctcgg
      181 catggccttc cagatcaccg acgacctgct cgacttcgtg ggggattcgg gccacatcgg
      241 caagtccacc ggcgccgaca tccgggacgg caagatcacg cttccgttga tccatgcgct
      301 ccgccaggcg ccgccagaga aggccaacgc catcggcgag tacatccggg ccaccgacat
      361 caccgaaccg gcctggaacg ccatcttcga tttcaccgcg gaattcggcg gcctcgacta
      421 cgggcgcgcc atggcgcaga aatacgccga tcgcgccgtc cgggcactcg acgaattcga
      481 cgactcgccc atcaagagca ccctcgcaac cgccgtcgat tatgtcgtcc accgccggcg
      541 ctgaacggga gcgtccgcgc cggacgggca aggccagcgc tgattcccac gccctcgccc
      601 ttgccgccgc gcgcgaagcg ctgaagaaga aggcccagga tgtcgtcgtg ctcgacctgc
      661 gcacgctgac cggcgtttgc gacttcttcg tgatcgtcac cggcacctcg gacgtgcagg
      721 tgcgggccat cgccgaccag gtggaagaag ggttgcgcga gcaggggcac gacgtgtggc
      781 acgtggaagg tcgcaccgcc ggcaagtggg tgctgctcga cttcgtcgac gtggtcgtcc
      841 acgtcttcca tcacgaaacg cgggagctct accagctcga acggctgtgg cgcgacgcgg
      901 cccgcgaaga aatggccgat gagtagcgcg cccctggcca ccatcgagca gcagctcgcc
      961 gcccgcatca acgacacgat cggccgcctc tggccggaat ccgcccatct cgtgccgaac
     1021 cccgaagtgg ccgtcccgcg cgaggccggg cacggcgatt acagcaccaa cgcggcgatg
     1081 ctgctggcca aggcgctcaa gaagaacccg cgggaatttg cgggggcgct ggcggaggag
     1141 ctgaagaagt cggacgacct cctggatggg gtggaggtgg cggggccggg attcatcaac
     1201 ctcacgctgt cgcggacggt tctcaacgac acgctgaagc gggtgctcgc cgaggggagc
     1261 gcctacgggt gcagcaatgc cgggctcggc gagcgacttc aggtggaatt cgtcagtgcc
     1321 aacccgaccg gtccgctcaa cgtcgtgaat gcccgcgcgg ccgctttcgg cggcaccctg
     1381 gcacgactct tcaacgccgc cggctggaag tgcgacaccg agttctacgt gaacgacgtc
     1441 ggcacgcaga tcgacctcct cggccagagc ctcctggctc gttatcggga gtcgaagggt
     1501 ggcggtcccg cggtcattcc cgagaacggt taccagggtc tttacctggc cgacatcgcc
     1561 ggggaactca agggccccga cgtggacgcc gcgctggcga agccgaatga caccgccatc
     1621 gccgccgact tcagcaagat cgccacggcc cgcatccgcg cctggcagaa gggcgacctg
     1681 gccaggtacg gcactgaatt cgaccgctgg tacctccagt ccgagctcta ccccgaagcc
     1741 gtgcagaaga cgctcgcctt catcaaggag cggggctacg tggtggaaaa agacggcgcc
     1801 caatggctcg ccaccacgcg tttcggcgac gaccaggacc gcgtcctcgt ccgccagacc
     1861 ggcgagccca cctacttcct ggccgacatc gcctatcacg tcgacaagca caaccgcggc
     1921 tacgcccacg tcatcgacat ctggggcccc gaccaccacg gccacatccc ccggatgcag
     1981 gccggcatgg aaatcctggg ctacggacgc gactggctcg aagtcatcat cgtccagcag
     2041 gtcaatctgc tcagcgcggg gcagccggtc aagatgagca agcgagccgg cgagctggtg
     2101 acattgagtg acctcgtcga tgacgtcggc aaagacaccg ccatcttctt cttcctgatg
     2161 cgccgcgcca gcagccactt ggattttgat ctcgatgtcg cgcgtaaaac gagcgacgag
     2221 aaccccgtct actacgtgaa gtacgcccac gccagaattg ccagcctctt acggaaggcc
     2281 gtggagtcct cccaacccgg agccaacgac ccggcagcgg gcgaatcctc gctgctgact
     2341 cattccaccg aactggacct gatgcgcctg ataacgcgcc tcccggaagt cattgtgggt
     2401 ggggcgcgac atagagagcc gcatcgcttg accaactacc tccgcgaggt cgcctccgcc
     2461 ttccacccct tctaccacca gtgccgcgtg gtgggcgagg agccgaagct cgcgcacgcg
     2521 cggctgctcc tctgcgccgc ggtcaagcag gtcctggcga acggcatggc gctgatcaac
     2581 gtcgacgctc cggaaagcat gtaggcggat gtccgaccag gaagccgaag tgcccggtcc
     2641 cgagatcgtt gccgtcccgc cgccgccgcc gtgggagccg atcaccccgg tcccggcatc
     2701 gaagcccttc ccgggttggt gggcgtcgat cggcctcctc gtggccatcc agttccttgc
     2761 gaacctggtc ggcttcatct tcctggcgcc gggcatcgtg tcggatgtca tggcacacgg
     2821 cacgctcacc cgcacgctgc cgcttgccgc ggtggggatc gtggccaact ccatcgcgtt
     2881 cggcctcgtc acctggttcg cctggcgcgc gtcgaagctg acggcggccg aggcgttccc
     2941 gctccgtcgc gtatcgggcg gcgcctggtt caccgcggcg ctcctcatgg ggggcacggt
     3001 ggtgctggcc gagttcgtgt cgagcggcat gatgaggctg tggcctcccc cggacttcgt
     3061 ccgcgacctc ttcctccagc tggtgggcga cgacgcgccg gtcgtcgtca gcatcatctt
     3121 cctggtgatc gtggcgccca tcaccgagga gttcttcttc cgcggcgtgc tgcagaaggg
     3181 tcttacccgg cgctacggtc ggaaggcggg agtcatcggg tcgggcctgc tgttcggcct
     3241 catccacctg atcccgtggc aggtggtgcc ggccgccctg ctcggcctcg tcttcgcgtg
     3301 gtggaccgac cggaccggca gcctctggcc ggcgctggtg gcgcacgcgg tcaacaacgg
     3361 catgagctat tacctcagcc gcagccagtc gggacgcgat gtcctcgaac cggggcccat
     3421 cgatcccgtg gccctggcca tcggggcggg cctcctcctg gcgggcctcg ccctttctcc
     3481 ccgattgctg cgaccgacgg aacccgaaac cgtcggcgaa aggagtttca cgtgagcgct
     3541 ctcgtcgtcg gctcggtggc cctcgatacc gtcatcacgc cgttcggccg ggccgacgag
     3601 gcgctgggtg gttcggcctc gttcttctcg gtggcggccc gcctcttcac gccggtaaac
     3661 ctggtggccg tggtggggga ggactttccg aaggcccacg tcgagttgct cgagtcgcgc
     3721 ggcgtgaaca ccgacggcct cgtcaccgcg cccggccgta cgttccgctg ggcgggggag
     3781 tacagcttcg acctgaacag ccggaccacc ctcgacacgc agctcaacgt cttcgccgac
     3841 ttcgatcccg tggtgccgga ggcctatcgg aacccccggt tcctcttcct ggccaacatc
     3901 gatccgggcc tgcagcggcg ggtcctcgac caggtgaacc gtcccgaaat ggtcctcatg
     3961 gacacgatga acttctggat cgagcgctcg cgcgagtcgc tgatggaaac gctgcgccgc
     4021 gtcgacctcg tcttcctcaa cgacggtgag acgcgcgaac tgaccggcga gtcgaacctg
     4081 atcaaggcgg cccgcgcggt cctcgacatg gggccctccg tcgtcgtcgt caagaagggg
     4141 gagcacggct cgctcctctt cacgcgcgat ttcaccttct cggcgccggc ttaccccatc
     4201 gagcacctct tcgatccgac cggggcgggg gataccttcg ccggcggctt cgtcggccac
     4261 ctggcccgat ccccccgtgt ggacgaatcg gaattgcgcc gggccgtcgt gaccggtacg
     4321 gtcatggcat cgttctcggt cgaatcgttc agcctcgatc ggctggccga tgtcacgatg
     4381 gacgaggtgg cggggcgcgt gcgcgag
//
LOCUS       DASVOD010000195         3337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_169635, whole genome shotgun sequence.
ACCESSION   DASVOD010000195 DASVOD010000000
VERSION     DASVOD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169635"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="VF720_04090"
     CDS             <1..1001
                     /locus_tag="VF720_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878561.1"
                     /translation="LSTAVIDSVSAQTSSPGDWPNFLMSALLPLAASIFFTMRNLRLG
                     RGDWRGATRIAVFVFVMNILEGATTTRLSEVGLIGAGADIVHGRAFGHALMHAVSMWF
                     AYVALEPYVRRLWPRTLVSWARLLSGRLRDPLVGRDLLIGAVAGSAMTALYLISASVF
                     RHFGLHAVPTLVGWEMPSSLTSLSNTACALSYGGSVCVLEVLGTFVQLLVLRLLFRHT
                     GAAIAATMAIAMGLTLIGAVPTEGWLIGIVGGLFRALPVLILMRFGVLAGVTTAFVAL
                     VVNSVVPSLDFSAWYADRALVPLVLLVGILIYGAFTALAGKSLFGDPLREERERGA"
     gene            complement(1044..3275)
                     /gene="katG"
                     /locus_tag="VF720_04095"
     CDS             complement(1044..3275)
                     /gene="katG"
                     /locus_tag="VF720_04095"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX7878562.1"
                     /translation="MSVNDSQETTGGRCPFTGGSTSRQGASHGRATTNANWWPDQLDL
                     KILHQHSSRSNPMDPGFDYAAEFRTLDLDSVVKDLHALMTDSQEWWPADYGHYGPLFI
                     RMAWHSAGTYRIGDGRGGAGHGAQRFGPLNSWPDNGNLDKARRLLWPIKQKYGRKLSW
                     ADLMILAGNVALESMGFRTFGFAGGRPDIWEPQEDIYWGSEGKWLADERYSGDRDLAH
                     PLAAVQMGLIYVNPEGPNGNPDPLASARDIRETFRRMAMNDEETVALTAGGHTFGKAH
                     GAGDPSHVGAEPEGATIADQNFGWLSTHGTGRGGDTITSGIEGSWTSNPTRWDTGYFE
                     VLFGYDWELTKSPAGANQWQARNLADRDTAPAAHDASKRVPLMMTTADMAMRMDPAYE
                     KISRRFRDDPKAFADAFARAWFKLTHRDMGPRSRYLGPLLPSEELIWQDPVPAVDHEL
                     VSDADVADLKDRILASGLPVSDLVGAAWASASTFRGSDKRGGANGARVRLAPQKDWEV
                     NQPGRLAHVISTLDGIRNSWNADQAGKKKVSLADLVVLGGGAAIEAAAKKAGTTLTVP
                     FTPGRMDASAEQTDVDAFSVLEPQADGFRNYARPGLGRYAAELLVDRAQLLTLTAPEM
                     TVLVGGLRVLGANTGASTHGVFTDRHGMLTNDFFVNLLDMGTKWTQVDEAGGILEGRD
                     RKTGELRWTGTIADLVFGSNSQLRAIAEVYASNDAGDRFVGDFVAAWTKVMELDRFDR
                     ARR"
BASE COUNT          458 a         1174 c         1093 g          612 t
ORIGIN      
        1 ggctctccac ggccgtcatc gactcggtgt ccgcgcagac ctcctcgccc ggagactggc
       61 ccaacttcct catgagcgcc ctcctgccgc tggcggcaag catcttcttc accatgcgca
      121 acctgcgcct cggccgcggc gactggcgcg gcgccacgcg catcgccgta ttcgtcttcg
      181 tgatgaacat cctcgaggga gccaccacca cgcgcctctc cgaggtgggg ctgatcggcg
      241 ccggcgccga cattgtccat ggtcgcgcct tcgggcacgc cctcatgcac gccgtgagca
      301 tgtggttcgc ctacgtggcg ctcgagccgt atgtccgccg gctgtggccc cgcacactcg
      361 tctcctgggc ccgcctcctc tccggccgcc tgcgcgatcc attggtcggg cgcgacctcc
      421 tgatcggcgc ggtggccggc tcggccatga ccgcgctcta tctcatttcg gcgtccgttt
      481 tccggcactt cggcctccac gccgtcccga cgctcgtggg gtgggagatg ccgtcctcgc
      541 tcaccagcct gtcgaacacc gcctgcgccc tctcgtatgg tggatcggtg tgcgtcctgg
      601 aggtcctcgg cacgttcgtg cagctgcttg tcctgcgcct cctgttccgg cataccggcg
      661 cggccatcgc cgccaccatg gccatcgcca tgggactcac cctgatcggg gccgtgccga
      721 ccgaggggtg gctcatcggc atcgtcggcg gccttttccg ggccctcccc gtcctgatcc
      781 tgatgcgatt cggcgtgctc gccggcgtga ccacggcctt cgtcgcgctc gtcgtcaact
      841 cggtcgtgcc atcgctcgat ttctcggcgt ggtacgcgga ccgggctctc gtgccgctgg
      901 tgttgctggt ggggatcctg atctatgggg cgttcacggc gctggcgggg aagtcgctct
      961 tcggggatcc cttgcgggag gaaagagaaa ggggagcctg agcaggcccc cctctccagc
     1021 acattcatgt ccgacggagt cgactaccgc ctggcccgat cgaagcgatc cagctccatc
     1081 accttcgtcc aggccgccac gaagtcgccc acgaaccggt cacccgcgtc gttcgaggcg
     1141 tacacctcgg cgatggcccg cagctgcgag ttcgagccga agacgaggtc ggcgatcgtg
     1201 ccggtccagc ggagctcgcc tgtctttcgg tcgcgtcctt cgaggatccc tccggcttcg
     1261 tcgacctgcg tccacttcgt ccccatgtcg agcaggttca cgaagaagtc gttggtcagc
     1321 atcccgtgcc ggtcggtgaa gacgccgtgc gtggacgcgc cggtattcgc accgagcacg
     1381 cgcaatccgc ccaccagcac cgtcatctcg ggcgcggtga gcgtgaggag ctgcgcacga
     1441 tccaccagca gctcggcggc gtagcgcccg aggcccggac gggcgtagtt ccggaagccg
     1501 tcggcctgcg gttcgagcac cgagaaggcg tcgacgtcgg tctgctccgc cgacgcgtcc
     1561 atgcgcccgg gcgtgaacgg cacggtcagg gttgtccccg ccttctttgc cgcggcttcg
     1621 atcgccgcgc cacctcccag cacgaccagg tcggccagcg agactttttt ctttcccgcc
     1681 tggtcggcgt tccaggaatt ccggatgccg tcgagcgtgg agattacgtg ggccagccgg
     1741 ccgggctggt tcacctccca gtctttctgc ggagccaggc gaacacgagc gccgttggcg
     1801 cccccgcgct tgtcgctgcc gcggaacgtc gaggccgaag cccaggccgc gccgacgaga
     1861 tcggaaaccg ggagcccact ggcgaggatg cggtccttga gatcggctac gtccgcgtcg
     1921 ctcaccagct catggtcgac cgccggaacc gggtcctgcc agatcagctc ttccgacgga
     1981 agaagcggac cgaggtaacg ggaacgcggt cccatgtcac gatgcgtcag cttgaaccac
     2041 gcgcgggcga acgcgtcggc aaacgccttc gggtcgtccc ggaagcggcg cgagatcttt
     2101 tcgtatgccg ggtccatgcg catggccatg tcggcggtgg tcatcatgag cggcacgcgc
     2161 ttcgatgcgt cgtgcgccgc cggagccgtg tcccggtcgg ccaggttcct ggcctgccac
     2221 tggttggcgc cggccgggct cttcgtgagc tcccaatcgt agccgaacag cacctcgaag
     2281 taaccggtat cccacctggt gggattggag gtccacgacc cctcgatgcc gctggtgatc
     2341 gtgtcgccgc cgcgcccggt tccgtgggtg cttagccagc cgaagttctg gtcggcgatg
     2401 gtggctcctt ccggctcggc accgacgtgc gagggatctc ccgcgccgtg ggccttgccg
     2461 aacgtgtggc cgccggcggt cagtgccacg gtctcctcgt cgttcatggc catccggcgg
     2521 aacgtctccc ggatgtcccg ggccgacgcc agcggatccg ggttgccgtt cggcccttcg
     2581 ggattcacgt agatgagccc catctgcacc gccgccaggg gatgcgcaag gtcgcgatcg
     2641 ccgctatagc gctcgtcggc gagccacttc ccttccgatc cccagtagat gtcctcctgc
     2701 ggctcccaga tgtcgggccg gccgccggca aagccgaatg tcctgaaccc catcgactcg
     2761 agcgccacgt tgccggccag gatcatgagg tcggcccagg agagcttgcg gccgtacttc
     2821 tgcttgatcg gccacaggag gcgccgcgcc ttgtcgaggt tcccgttgtc gggccagctg
     2881 ttgagcggac cgaaccgctg ggcgccgtgc cccgcgccgc cgcgtccgtc accgatacgg
     2941 taggtgcccg cgctgtgcca ggccatgcga atgaacaggg gaccgtagtg cccgtagtcg
     3001 gccggccacc actcctgcga gtcggtcatg agggcatgca ggtccttgac caccgagtcg
     3061 agatcgagcg tcctgaactc ggccgcgtag tcgaaccccg ggtccatcgg gttcgagcgg
     3121 gaggagtgct ggtgcaggat cttcaggtca agctgatcgg gccaccagtt ggcgttcgtg
     3181 gtggcccgcc catgcgaggc accctgccgc gaggtactgc cgcccgtgaa cgggcacctg
     3241 ccgcccgtcg tttcctgact gtcgttgacg ctcatgaacc atcctttccg aagtgccgga
     3301 tgtccgtaac gggaaaggag acagggtcgc cgcggat
//
LOCUS       DASVOD010000196        16639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_377421, whole genome shotgun sequence.
ACCESSION   DASVOD010000196 DASVOD010000000
VERSION     DASVOD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377421"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VF720_04100"
     CDS             complement(<1..1579)
                     /locus_tag="VF720_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX7878563.1"
                     /translation="MKRAAHSRPSPPALTRALLALLAFALALRLTGIGFGLPNARHAY
                     PYNPDEWTPMQVLKGMDPGRLDFNPHYYFNPTLQYYLYGATFAAAHVAGWINIKGDER
                     FYFQHPENIARMILLGRLWSALFGVATVWLGYRLARRIGASRNTALGAAALLAVQPAH
                     VIHGHFMTVNSPVTFWMTLALLLLVRWLGRPGIVAAALAGAAIGLSITTKFSAGILVP
                     LAGFAALLVWRRRGFGHAMGESVALVVAALAAFVAGTPYAVLAWPEFRDGFIEMTTYF
                     QAGPPPGGPMAGLMKVLRMYFYAVTPGVLLAGIGGLLLPRLRRSAGGLLLIVFIAAFM
                     ATTIKAGALASDSRFLPLFPALCVTAALAFAALRKARFALGSAALAVSLAFVALADIA
                     YLGRFIGRMPQETASDWMRSHVTGSPLIHLAGTSIYYFPDLPLRERLAGENPGAYARE
                     TEWRFVESKEFATARPANPDVVVLTSWTARKPEELEWLSQPDYDVVATFPGKVNFFGK
                     RWPVPLDLYDTDTWILRR"
     gene            1681..1757
                     /locus_tag="VF720_04105"
     tRNA            1681..1757
                     /locus_tag="VF720_04105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1715..1717,aa:Arg,seq:ccg)
     gene            1785..3545
                     /locus_tag="VF720_04110"
     CDS             1785..3545
                     /locus_tag="VF720_04110"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7878564.1"
                     /translation="MRNIRLLPGFVLSLGLLALLAGLPGSNAAGPPAQPTADAARMLN
                     FVRHFSEDGSSQPISRYAYRVEMDALQADLRNQMAEALGPRGTAELWAFPSRMPPGIW
                     DPQAALACGALDPLSPVHAHPTFWPAGGTIPFRSTFNNVIGRLPGTNPGAGKFVICSH
                     FDAIGFRTPGWQAWADPAPGADDNLSGTAAVVELARLLASEAPLPFDLEFVCFDGEEL
                     GLWGSDTLAHAERKANAPILGVFNMDMVGFNPVADSLVIMPNRESWYLADFVRETEAL
                     DPQPSLSLQVTIDNLVNSDHGPYWLQGYTAFMMIENLNVVRNNPQYHRVTDRASTLRG
                     GVMMAKAANVYLKALRRLAERSGGPPDLRISETDLIMFVNGSPEGRVAVPGDEIRVEG
                     GIFNAGGSTEGMPIHVSWYSIGNDGKRNYLETTTVTTPVRNGGHLRVPFVRTATAADV
                     GALTIEMELDDGTHIDTARRAIPVKDTVGRVVSHYMAPNPVRAIESASLQYELSREAD
                     VRITLFDAHGTELGRRYYEDVSGTRQTSVATNTGLNRVPLADLFGAFNPAPGIYLYRI
                     EVFGDDAVDEMTMGKFALLR"
     gene            3542..3772
                     /locus_tag="VF720_04115"
     CDS             3542..3772
                     /locus_tag="VF720_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878565.1"
                     /translation="MKPGPGDRFLIRLLLGLNLAMLVALPVLAVTLWRELGGPAWRDA
                     ARFRPLVVLVAAGGEFLLIRRFRGILARLRGI"
     gene            3918..4955
                     /locus_tag="VF720_04120"
     CDS             3918..4955
                     /locus_tag="VF720_04120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7878566.1"
                     /translation="MQQGTERRILIRTLHRIAELMAGDRDLERIQAAVLDGALELFGA
                     ETAFLLIREGEGDLRLARRRANIDQTSGLEAERFASSMFLTTLLSGRKPLTVADPANL
                     SSLGLGGIGFGESSPRNLVAVPFLARGNNQGALVVVNGLRLEHGNDEAMDLLAVLANQ
                     AAVAAETFRLNRRLEQLAITDELTQVYNYRFLKTALRKEVKRAGRFGHEFAILMIDVD
                     NLKKYNEKNGHLRGSELLRKVGRQLLDNSRAADLVAKYGGDEFLIILPLTGSEGAAIM
                     GERVRKAVAETAFVNAEPGVITISIGVAVFPGHGRSVEDLIASADEALFRAKRGGRNC
                     VQAAPPERQAA"
     gene            5004..5807
                     /locus_tag="VF720_04125"
     CDS             5004..5807
                     /locus_tag="VF720_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878567.1"
                     /translation="MSNDVLWLILSYVAVFALLAVGTWAGKRGGSPEITRKIIHIGVG
                     TWIIPTTLGFDSAWMAIIPPASFVVLNLISYRYRLVKAMEVGDRSPGTIYFPLAFVML
                     ILVFWPGGFFVRGLLTSGGPEFVPRIDTTPAPPVDGLPMRQVDILARLPALAGILVLA
                     WADAAAAILGRAYGRHSYRVLGATRSWEGSLAFLGFSALAMAAATLVTDLARLVPAST
                     AVGSALFSLPRFLLLMVPATIVEAVTPAGLDNLAVPAIVAATVALPYWL"
     gene            5821..6828
                     /locus_tag="VF720_04130"
     CDS             5821..6828
                     /locus_tag="VF720_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7878568.1"
                     /translation="MSRIFLTGATGYLGGRLAARFAADGHDLTLLLRDASRAPALPGV
                     SYRTAIGDLDDPHPDATRRLADAMSGHDTVVHTAAMVRNWAKDAAAFERVNVGGSWTM
                     CEAALAAGVGRFLYTSSFFALGPSLDGRPVTELALDLPAPARFFNEYHSTKFRAARHL
                     RDFIPRGLNLVTLIPTVIFGPGAETDGNHVARILRWLQRGQFPGHLGGARFRWNMAFV
                     EDVVNGHALALEKGKAGEAYILGGEDRMLRDVAETAARKLGVPIPTRNIPWGIGRLIA
                     GIEEWRAGTFGGSPRLSRGEIDIYRHEWIYSSQKAERELGYRITPFTDALDRTVAWIR
                     S"
     gene            6834..7562
                     /locus_tag="VF720_04135"
     CDS             6834..7562
                     /locus_tag="VF720_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEX7878569.1"
                     /translation="MEKLLEAIMHRGMLALGVNVVLALVAARAGTVRISGVVAGIIVG
                     SLIWLAGGWRWWLVLVAFFVLGSLLTKFGYRKKAALGLAEEKGGRRGWQNAVANGGVG
                     VLAGLAWLLTDWEPLRAAFAASFATALFDTAGSEIGGLYGRTPISLRSFRKVPPGTEG
                     AVSAEGTAGAFVFALLVALAALAAGQIPISFLLSVVLGSVIGGLYESFAAGRLPLGHQ
                     ALNFTNTLFGALAALALAWVTTRP"
     gene            7730..9106
                     /locus_tag="VF720_04140"
     CDS             7730..9106
                     /locus_tag="VF720_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7878570.1"
                     /translation="MLFRRAGPAENRKSRRLADPLERSASLTDLIKKIESRTAHCSVI
                     GLGYVGLPLAVEMARAGFTVTGFDVDVRKVDGINSGVSHVKDVADATLAEQVTAGRLR
                     ATTDFAKLAEVDTINICVPTPLRKTKDPDVSYIVAAVDQIVDHLRPGQLILLESTTYP
                     GTTDELILPRLEEKGLTVGKDFFLAFSPERVDPGNAKYQTHNIPKVVGGVTEACTAVA
                     SALYQTSIETVVPVSSTRVAEMVKLLENTYRSVNIGLVNELALLCDRMQIDVWEVIDA
                     AATKPFGFMPFYPGPGLGGHCIPIDPFYLSWKAKETGFEARFIELAGQINASMPHYVV
                     DRVAQVLNDAAKPVKGSKLLILGVAYKSDIDDFRESPALDVITELQKRGAVVEYHDPY
                     IPAFEHDHLKMTGVALTADRLAAADCVVITAAHKSIDWELVAGKSKAIFDTRNVFKGR
                     RDPKIARI"
     gene            9111..10100
                     /locus_tag="VF720_04145"
     CDS             9111..10100
                     /locus_tag="VF720_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7878571.1"
                     /translation="MLTPDAKVLVTGGAGFIGSHLVERLLGEGNHVRVFDNFSTGRAE
                     NLEQVREAVGSAAAARLEVHTGDLRNAADVTKAVQGMSVVFHEAALGSVERSVEDPLT
                     TNEVNTTGTLRLLVAARDAGVSRLVFAGSSSVYGEVEGLPKTEEMATIPMSPYGTTKR
                     DGELYCRQFAMLYGFNSVTVRYFNVFGPRQNPNSQYAAVIPIFIDRIMRGEAPIINGD
                     GEQSRDFTYVANVVDANLLAAKAPAESVRGEILNMACGTRYSLNQLVALVAGLTGKEV
                     APQYRAARAGDVRHSEADISRARRILGYEPRVSFEEGLRQTVEYFQKSVVPLP"
     gene            10142..11326
                     /locus_tag="VF720_04150"
     CDS             10142..11326
                     /locus_tag="VF720_04150"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX7878572.1"
                     /translation="MRRVLERNIQVANQPSGQPMVGMIDLKAQYGPLRPEIDAAVKNI
                     FDTGRFILGPEVEAFEKEIAELHGVPHGIGVASGTDALMLVWRALDLKPDDEVVVPSM
                     TFLASGSSVRLAGATPVFADVDPTTLNMTAATARAAVTKKTKAILVVHLYGQCANVPA
                     LAALADEIGVPLIEDCAQSIGATWDGVPCGSIGLAGTTSFYPSKNLGAPGEGGMVWSR
                     DEAFAARVRRLRNHGERVRYEHVDLGTNSRLSAINGAVLRIKLRALPKWTELRRENAA
                     FYDAAFANLGPTIRLMTVDPRAKHVYHQYSFFSPERDKLREHLTAAGIENIVYYPIPL
                     HRQICFADLKADPANFPNTEMACREVLSVPVHSELTAAQRQRVADAVVSFAKAGATTG
                     GR"
     gene            11328..12374
                     /locus_tag="VF720_04155"
     CDS             11328..12374
                     /locus_tag="VF720_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013440582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7878573.1"
                     /translation="MARRILVTGGAGFIGSHVAEQYALDGWQVRVLDNLSRAKLLKKS
                     DRNASFNWDHLSKLPGVELIKGSVTDWETVRQAAADCQVIVHAAAQTAVTTSVTDPRP
                     DFENNIVGTFNVLEAAREQSVKPAIVFCSTNKVYGENVNGVGLSEGEKRWSFESSMAR
                     GIPETFPIDHCEHTPYGVSKLTGDLYMQDYAHLYGLKIGVFRMSCIYGTRQFGLEDQG
                     WVAWFTIASMLGKPITIFGDGKQVRDVLYVEDLVGAYRAFLDSSLNHAVFNMGGGPEF
                     TMSLHELLDILKARTGITPALSYADWRPSDQKVYISNIDKATKALGWKPSVTPEQGVA
                     KLIDWVKENKGLFA"
     gene            complement(12506..13078)
                     /locus_tag="VF720_04160"
     CDS             complement(12506..13078)
                     /locus_tag="VF720_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878574.1"
                     /translation="MKSWRPFLALPLLATLVTLTAAAPPLGIINGDFSAGNVGWNVNQ
                     PANWTVSFPAAGGNPDAYGRIQSPFGNSGGTGSIFQTFLCGEPGIPGNCVITLDYRLD
                     QLDASQLSGRVVVRIDGTVEYTWSLGPVGWTNVTVTVPCGNHVISLELEVSAGNNGWA
                     ACFDNVRASCEPIVPTRSSTWGRIKSLIIE"
     gene            complement(13172..14071)
                     /locus_tag="VF720_04165"
     CDS             complement(13172..14071)
                     /locus_tag="VF720_04165"
                     /inference="COORDINATES: protein motif:HMM:NF041383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPGUxxT family thioredoxin-like (seleno)protein,
                     type 2"
                     /protein_id="HEX7878575.1"
                     /translation="MAIQRVLTFLLTTLVAVVIPATLAAGGNPRELGRVSWLRDWNEA
                     AAHSRAEGRPLFVLFQEIPGCATCVGFGQTVLSNPRLVEGIETLFVPLAIHNNKGGAD
                     QVVLERFGEPAWNNPVVRFMAADGKDWIARKDGVWSEAEVARRMVAALDASGHAAPEW
                     LHVVADELDVDDARHLYLAMHCFWEGEARLGSIEGVLATRAGFIDGQEVVDVTYDAGH
                     LDAATLIAEADRRGVAGKAWRDGDPGFREAPPSDQKRHLSGTRWEKAPDLTPLQAARI
                     NSALADGKDPAAWLTPRQAAALK"
     gene            complement(14050..14670)
                     /locus_tag="VF720_04170"
     CDS             complement(14050..14670)
                     /locus_tag="VF720_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878576.1"
                     /translation="MTLRIAFLALVLALVTPPMAAGQVVNGAFEDPDEAGWIDDLPVG
                     GWTATFPDSGGVTGGCARIASPTPDGEDYGCLSQLFSCGEPGSDAVCLISVTYRHANL
                     GAAPRTGGVTIRLDGTTILTTDPEEIQAGWRTAAVTVPAGTHTLALCLYRLDGPDLDG
                     APSLRRDIGGIEGWEAYFDDVTAIVTPSSPTRTTTWGKLKWQFSGS"
     gene            14931..>16639
                     /locus_tag="VF720_04175"
     CDS             14931..>16639
                     /locus_tag="VF720_04175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX7878577.1"
                     /translation="MATARRFPEDPLERRLDEIQGLLREPLIPDDADLSSPATLSDLA
                     LILADRVERGHRQLIETSVQLTSLREVAHRLLSVRSADRAAETIALYLHKAFDHGRVA
                     VLLFDREALTLRGTAIRRAGLGFFQAQVALPIAGAQGAIPATFASGEARLIDSPSEAN
                     LYDGEGDLAGAFDMEDLGSIVVAPLKAAHLFGSPVSNGGWRAGEEAANPLDPRHTILG
                     LILVGRSRRESPLTSDDLAGIESVATTLSTVVENGRLYQDLRQGEMFRDDLLNSMGSG
                     LVAINLARRAISMNQTAVGLTGWEISDVLGDVPPFLPIADRGMDAAIRGASYGKPVLR
                     KEVVLSRKDGTFFPASVSTSPLRNEDGEIYGVIATFQDLTVFKEMEERIRQLDRLAAL
                     GRFTAAIAHEIRNPLAGISAGVEYLTKGMSQAGDAADNIRFIQTEIKRLNQIIEDLFR
                     VTHPRPLMTSPERPEAILERSLRCLGEMANERKIEVVRILPDSVPAVPVDADQIQQVA
                     INLIKNAIEAMEPGGTLTMRTGTRPGRPGEEPWITLSVSDTGPGMSAEVRKRLFEPFF
                     TKKA"
BASE COUNT         2694 a         5745 c         5585 g         2615 t
ORIGIN      
        1 gccggcgcaa tatccaggtg tccgtgtcgt acaagtcgag gggcaccggc cacctcttcc
       61 cgaagaagtt cacctttccc gggaacgtcg ccaccacgtc atagtcgggc tggctgagcc
      121 actccagctc ctcgggcttc cgcgccgtcc agctcgtcaa caccaccacg tccgggttgg
      181 ccggccgcgc ggtggcgaat tccttcgact ccacgaagcg ccactcggtc tcacgcgcgt
      241 aggcgccggg gttctcgccg gccaggcgct cgcggagcgg caagtcgggg aagtagtaga
      301 tcgacgttcc cgccaggtgg atgaggggcg atccggtcac gtggctccgc atccagtcgg
      361 aggcggtctc ctgcggcatc cggccgatga agcggccgag gtacgcgatg tcggccagcg
      421 ccacgaacgc cagcgagacg gccagggccg cggagccgag cgcgaagcgg gccttgcgga
      481 gtgccgcgaa agccagcgcg gccgtcacgc agagtgccgg aaagagcggg aggaagcggc
      541 tgtcactggc cagggcaccg gccttgatcg tcgtggccat gaaggcggcg atgaagacga
      601 tgagcaggag ccccccggcc gagcggcgga ggcgcggcag caagaggccg ccaattcccg
      661 ccagcaggac gccgggagtc actgcataga aatacatgcg gagcaccttc atgagcccgg
      721 ccatggggcc gccgggcggc ggcccggcct ggaagtaggt ggtcatctcg atgaagccgt
      781 cgcggaactc cggccaggcg agcaccgcgt acggcgttcc ggccacgaag gcggccagtg
      841 ccgccaccac gagcgccacc gattcgccca tggcgtggcc gaagccgcgg cgccgccaca
      901 ccagaagcgc cgcgaagccg gcgaggggca cgaggattcc ggccgagaac ttcgtcgtga
      961 tcgacaggcc gatcgcggcg ccggccaggg ccgccgcaac gattcccggc cgtcctaacc
     1021 aacgtacgag caggaggagc gccagcgtca tccagaacgt caccggcgaa ttcacggtca
     1081 tgaagtggcc gtggatgacg tgcgccggct gtaccgcaag gagcgcggcg gcgcccaggg
     1141 ccgtgttgcg cgaggccccg atccgccggg ccagccggta ccccagccag accgtggcca
     1201 cgccgaagag tgccgaccag agtcgcccga gcaggatcat ccgggcgatg ttctccgggt
     1261 gctggaagta gaagcgctcg tcccccttga tgttgatcca gccggccacg tgggcggccg
     1321 cgaaggtggc gccgtagagg taatactgca gcgtggggtt gaagtaatag tgcgggttga
     1381 agtcgagccg cccggggtcc attcccttca ggacctgcat gggcgtccac tcgtcggggt
     1441 tgtaggggta ggcgtggcgg gcattgggga ggccgaaccc gatccccgtg aggcggagcg
     1501 cgagcgcgaa tgccaggagc gccagcaatg cacgcgtcag ggccggcggg gagggacggg
     1561 aatgcgcggc acgcttcatg gcgcggcagc atacggggcg gcgcgcggcg gcgccacctt
     1621 gaaatgggag ggcggtcgtg gcacagttcc cagccgatcg acgcctgtcg gtcgtccagt
     1681 gcgcccgtag ctcagctgga tagagcacca gcctccggag ctgggtgtcg gaggttcgaa
     1741 tcctctcggg cgtaccactt tccgggattt gcggagagcg ccgcgtgcga aatatccgtc
     1801 tgttgcccgg gttcgtcctc tcgctcggac ttcttgccct cctggcgggc cttcccggtt
     1861 ccaatgccgc cgggccgccg gcccagccca ccgccgacgc cgcacggatg ctcaatttcg
     1921 tgcgtcactt ttccgaggac ggctccagcc agccgatcag ccgctacgcc tatcgggtgg
     1981 aaatggacgc cctccaggcc gacctccgga accagatggc cgaggcgctc ggcccgcgcg
     2041 gcaccgccga gctctgggct ttcccgtccc ggatgccgcc gggcatctgg gatccccagg
     2101 ccgccctggc ctgcggggcg ctcgacccgt tgtcgcccgt ccacgcgcac cccaccttct
     2161 ggccggccgg cggcacgatt ccgttccgct cgaccttcaa caacgtgatc ggtcggcttc
     2221 cgggcacgaa cccgggcgcc ggaaagttcg tcatctgctc tcatttcgac gccatcggat
     2281 tccgcacccc cggctggcag gcgtgggccg atccggcgcc cggtgccgac gacaacctga
     2341 gcggcacggc cgcggtcgtc gagctggcgc gcctcctggc ctccgaagcc ccgttgccct
     2401 tcgacctcga gttcgtctgc ttcgacggcg aagagctcgg cctctggggg agcgacacgc
     2461 tggcccacgc cgaacggaag gccaatgccc cgattctcgg cgtcttcaac atggacatgg
     2521 tcggcttcaa cccggtggcc gattcgctgg tcatcatgcc caaccgcgag tcgtggtacc
     2581 tggccgactt cgtgcgcgag acggaggcgc tcgatccgca gccgagcctc tcgctccagg
     2641 tgacgatcga caacctcgtg aacagcgatc acggcccgta ctggctgcag ggatacacgg
     2701 ccttcatgat gatcgagaac ctgaacgtgg tccgcaacaa cccgcagtac caccgggtca
     2761 ccgaccgcgc ctccacgctc cggggcggcg tcatgatggc caaggcggcc aacgtctacc
     2821 tcaaggccct gcggcgcctg gccgagcgat cgggtggacc gccggatctc cgcatcagcg
     2881 agaccgacct catcatgttc gtgaacggct ccccggaggg gcgcgtggcc gtgccggggg
     2941 atgagatccg cgtcgagggg ggaatcttca acgccggcgg ttccacggag gggatgccca
     3001 tccacgtgag ctggtattcg atcgggaatg acggcaaacg caactacctc gagacgacca
     3061 ccgtcacgac gcccgtccgg aacggcggac acttgagggt gccgttcgtc cggaccgcca
     3121 cggcggccga tgtcggcgcc ctgacgatcg agatggagct cgacgacggc acgcacatcg
     3181 acaccgcgcg ccgggccatc ccggtgaagg acacggtcgg gcgcgtcgtc agccactaca
     3241 tggccccgaa cccggtgcgc gccatcgagt cggcctcgct gcagtacgaa ctgtcgcgcg
     3301 aagccgacgt ccgcatcacg ctgttcgacg cgcacggcac cgaactcggc cggcgctact
     3361 acgaggacgt gagcggcacc cgccagacaa gcgtggccac caacaccggc ctgaaccgcg
     3421 taccgctcgc cgacctgttc ggcgctttca accccgcccc cggcatctac ctgtatcgca
     3481 tcgaggtctt cggcgatgac gccgtcgacg agatgacgat gggcaagttc gcccttctca
     3541 ggtgaagccc ggtccgggcg accgcttcct catccggctt ctcctcggct tgaacctggc
     3601 catgctggtg gccctgccgg tccttgccgt caccctctgg cgggagctcg gtggcccggc
     3661 ctggcgcgat gccgcccgat tccgccccct ggtggtcctg gtggccgcgg gaggggaatt
     3721 tctgctcatc cggcggttcc ggggcatcct cgcgcgactt cgggggattt gaccccgtcg
     3781 gaaaccccga tgcggtgcga attgtcttcg gcggggctca agacccgcca tggggcgggc
     3841 gataactgag acgacacgag ttggtcgtcc acgccctgga accaccgttc ggccgcgcga
     3901 acgtgacggg agatgtcttg cagcaaggga ccgaacgacg catcctgatc cgcaccctac
     3961 atcgcatcgc cgagctcatg gccggcgatc gcgacctcga gcgcatccag gcggccgtgc
     4021 tggacggcgc gctcgaactc ttcggcgccg aaaccgcctt cctgctcatc cgcgaagggg
     4081 agggtgacct ccgcctggct cgccgccgcg ccaacatcga ccagacctcg ggcctggagg
     4141 cggagcgctt tgcgtcgtcc atgttcctga ccactcttct ctcggggcgg aagccgctca
     4201 cggtggccga tccggccaac ctgtcgtcac tcggcctggg cggcatcggc ttcggcgagt
     4261 cgtcgcctcg caacctcgtg gccgtcccct tcctggcccg cggcaacaac cagggcgcgc
     4321 tggtcgtggt gaacggcctg cgcctcgaac atggcaatga cgaggcgatg gacctcctgg
     4381 ccgtgctggc caaccaggcg gccgtggccg ccgaaacgtt ccgcctgaat cgccgcctcg
     4441 agcagctggc catcaccgac gagctcacgc aggtctacaa ctaccgtttc ctgaagaccg
     4501 cgctgcgcaa ggaagtgaag cgggcgggcc ggttcggcca tgaattcgcg atcctgatga
     4561 tcgacgtgga caacctgaag aagtacaacg agaagaacgg ccacctccgc ggctcggaac
     4621 tgctccgcaa ggtcggccgg cagctcctcg acaactcgcg ggccgccgac ctcgtggcca
     4681 agtacggcgg cgacgaattc ctgatcatcc tgcccctcac cggcagcgag ggggccgcca
     4741 tcatgggcga gcgcgtccgg aaggcggtgg ccgaaaccgc cttcgtgaac gcggaaccgg
     4801 gcgtcatcac catttcgatc ggcgtggccg tcttcccggg ccacggacgc tcggtcgaag
     4861 atctcatcgc cagcgccgac gaggcgctct tccgcgccaa gcgcggcgga cgcaactgcg
     4921 tgcaggccgc gcctccggaa cgccaggcag cctgacaacc ccgtcagctt gcccgtcgct
     4981 ccggccggtg gtctaatcgt cccgtgtcca acgatgtcct ctggctgatc ctctcgtacg
     5041 tcgccgtctt cgcattgctc gcggtcggca cctgggccgg caagaggggc ggctcccccg
     5101 aaatcacccg caagatcatc cacatcggcg tcggcacctg gatcatcccc accacgctcg
     5161 gcttcgacag cgcctggatg gccatcattc cgcccgcctc gttcgtcgtc ctgaacctga
     5221 tttcctatcg gtatcgactg gtgaaggcga tggaggtggg agaccggagt ccggggacga
     5281 tctactttcc gctggcgttc gtgatgctca tcctggtgtt ctggccgggc gggttcttcg
     5341 tgcgtgggct cctgacgagc ggaggcccgg agttcgtccc ccgcatcgac acgacgccgg
     5401 cgccacccgt cgacggcctg ccgatgcgcc aggtcgacat cctcgccaga ctccccgcac
     5461 tggccggcat cctggtcctg gcctgggcgg acgcggcggc ggcaatcctg gggcgtgcgt
     5521 atggccgcca ctcttaccga gtgctcggcg ccacccgttc ctgggagggc tcactggcct
     5581 tcctcggttt ctcggcgctg gcgatggcgg cggccacgct cgtcaccgac ctggcgcgcc
     5641 tcgttccggc aagcacggcg gtggggtcgg cgctgttcag tctcccgcgc ttcctgctgc
     5701 tcatggtgcc cgccacgatc gtcgaagcgg tcacgccggc cggcctcgac aacctcgccg
     5761 tccccgccat cgtggccgcc acggtggccc tcccgtactg gctctgagga actggggacg
     5821 atgtcgcgca tcttcctgac cggcgccacc ggctatcttg gagggcgact ggccgcccgg
     5881 ttcgccgccg atggccacga tctcacgctg ctgctgcggg atgcctcacg agcgcctgct
     5941 ctgcccggcg tttcctaccg caccgccatc ggcgacctcg acgaccccca cccggatgcc
     6001 acccgacgcc tggccgacgc gatgtccggg cacgataccg tggtgcacac ggcggccatg
     6061 gtcaggaact gggcgaagga tgccgcggcc ttcgagaggg tgaacgtcgg ggggagctgg
     6121 acgatgtgcg aagcagcgct ggccgccggg gtgggccggt tcctgtacac gtcgtcattc
     6181 ttcgcgctcg ggccgtcgct cgatggccgc ccggtcacgg aattggcgct cgacctgccg
     6241 gcaccggccc gatttttcaa cgaatatcac tccaccaaat tccgggcggc ccgccacctg
     6301 cgcgatttca tcccgcgggg cctgaacctc gtcacgctca tcccgaccgt catcttcggc
     6361 cccggcgcgg agaccgacgg caaccacgtg gcgcgcatcc ttcgttggct gcagcgcggc
     6421 cagtttcccg gacacctggg aggcgcgcgc ttccgttgga acatggcctt cgtggaggac
     6481 gtggtgaacg ggcacgcgct cgcgctcgag aaggggaagg cgggcgaggc ctatatcctc
     6541 ggcggcgagg accgcatgct gcgcgacgtg gccgaaacgg ccgcccgaaa gctcggcgtg
     6601 ccgatcccga cccgcaacat cccgtggggt atcggccgcc tcatcgcagg tatcgaggag
     6661 tggcgggccg gcacgttcgg cggatcgccg cgcctctcgc gcggcgagat cgacatctat
     6721 cgccacgaat ggatctactc gagtcagaag gcagagcgcg agctcggcta ccgcatcacg
     6781 ccgttcaccg acgcgctcga tcgcacggtc gcatggatcc ggtcgtgacg ccaatggaaa
     6841 aactcctcga ggcgatcatg caccgaggca tgttggcgct cggcgtcaac gtcgtgctcg
     6901 cactggtcgc ggcgcgcgcc ggcacggtgc ggatctcggg cgtcgtggcc ggcatcatcg
     6961 tcggatccct gatctggctg gccggcggct ggcgctggtg gctggtcctc gtggccttct
     7021 tcgtcctcgg cagcctgctc accaagttcg gctaccggaa aaaggccgcc cttggcctcg
     7081 ccgaggagaa ggggggacgc cgcggctggc agaacgccgt ggccaacggg ggcgtgggcg
     7141 tcctcgccgg actcgcctgg ctcctgaccg actgggagcc gctccgcgcc gccttcgcgg
     7201 cctcgtttgc caccgcgctg ttcgacacgg ccggctcgga gattggcggc ctgtacggca
     7261 ggacgccaat cagcctccgc agcttccgga aagtgccgcc gggcaccgag ggggccgtgt
     7321 cggcggaagg aacggccggg gcattcgtct tcgccctgct ggtggcgctg gccgccctgg
     7381 ccgccgggca gatcccgatc tcatttctac tgtcagtggt cctcgggagc gtgatcggcg
     7441 gtctctacga gagtttcgcc gccgggcgcc tgccgctggg gcaccaggca ctcaatttca
     7501 ccaacacgct gttcggtgcg ctggccgccc tggcgctggc gtgggtgacg acccggccct
     7561 agcgcgtgcc ggcgtctgtc ttgcttggta agacatcgcg cgaatgttcc aaccctgggg
     7621 cgagaccgcc ccggaattgc ctgtccgggc ctgctaagtt caaatcctgc aataagtact
     7681 ggccttccag cgccccgttt gttacacttc ttccttccaa atcgcccgaa tgctgtttcg
     7741 aagggccgga ccggccgaaa acaggaaatc caggcgcctg gccgacccgc tcgaaaggag
     7801 tgcttccctg acggacctga tcaagaaaat cgagtcgcgc accgctcact gttcggtgat
     7861 cgggctcggc tacgtaggac tcccgctggc ggtggagatg gcccgggcgg gtttcacggt
     7921 caccggtttc gacgtcgacg tccgcaaggt cgacgggatc aattcgggcg tgtcccacgt
     7981 gaaggacgtg gccgacgcca cgctggcgga gcaggtcacc gccgggcggc tccgggccac
     8041 caccgacttc gcgaagctgg ccgaggtcga caccatcaac atctgcgtgc cgacgcctct
     8101 ccgcaagacg aaggacccgg acgtgtcgta catcgtggcg gcggtcgacc agatcgtcga
     8161 tcacctgcgg cccgggcagc tcatcctcct cgagagcacc acctatccgg gcacgaccga
     8221 cgagctcatc ctgccgcgcc tcgaggagaa ggggctcacg gtggggaagg acttcttcct
     8281 ggccttcagc cccgagcggg tcgatcccgg caatgcgaag taccagacac acaacattcc
     8341 gaaagtcgtc ggtggtgtca cggaagcgtg taccgcggtg gcgtcggctc tctaccagac
     8401 ctccatcgag acggtcgtgc cggtgtcgag cacccgggtg gccgagatgg tgaagctcct
     8461 cgagaacacc taccgcagcg tcaatatcgg cctcgtgaac gagctggcgc tcctctgcga
     8521 ccggatgcag atcgacgtct gggaggtcat cgacgccgcg gccaccaagc cgttcggctt
     8581 catgcccttc tacccggggc cggggctcgg cgggcactgc atcccgatcg acccgttcta
     8641 cctgtcgtgg aaggccaagg agacgggctt cgaggcccgc ttcatcgaac tggccgggca
     8701 gatcaacgcc tcgatgccgc actacgtagt ggaccgggtg gcgcaggtgc tgaacgacgc
     8761 ggccaagccg gtgaaggggt cgaagctcct catcctcggc gtggcctaca agtccgacat
     8821 cgacgatttc cgggagagcc cggcgctcga cgtgatcacc gagctccaga agaggggcgc
     8881 ggtcgtcgag taccacgatc cgtacatccc ggccttcgag cacgaccatc tcaagatgac
     8941 cggcgtggcg ctcaccgccg accgcctggc cgcggccgac tgcgtggtga tcacggcggc
     9001 gcacaagtcg atcgactggg agctggtggc cgggaagtcg aaggccatct tcgacacgcg
     9061 caacgtcttc aagggtcgtc gcgacccgaa gatcgcccgg atctaggcgg atgctgaccc
     9121 cggacgcaaa ggtcctcgtc accggcggcg ccggcttcat cggcagccac ctggtggaac
     9181 gactcctcgg cgaggggaac cacgtccgcg tcttcgacaa cttctcgacc ggccgcgccg
     9241 agaacctgga gcaggtgcgc gaggcggtgg ggagcgccgc ggcggcccgt ctcgaagtgc
     9301 acaccggcga cctgcgcaac gcggccgacg tcacgaaggc cgtgcaggga atgagcgtcg
     9361 tcttccacga agcggcgcta ggatcggtgg agcgctcggt ggaagacccg ctcaccacca
     9421 acgaggtcaa caccaccggt acgctccggc tgctggtggc cgcgcgcgac gccggcgtgt
     9481 cgcgcctcgt cttcgccggc tcgtccagcg tgtacggcga ggtggaaggg ctccccaaga
     9541 ctgaggagat ggccaccatc ccgatgagcc cgtatggcac caccaagcgg gacggcgagc
     9601 tctactgccg ccagttcgcg atgctctacg gcttcaactc ggtcacggtc cgctacttca
     9661 acgtcttcgg gccgcgccag aatccgaaca gccagtacgc ggcggtcatc ccgatcttca
     9721 tcgaccggat catgaggggc gaagccccga tcatcaacgg cgacggcgag cagagccgcg
     9781 acttcaccta cgtggccaac gtcgtggacg ccaatctcct ggccgcgaag gcgccggccg
     9841 agtccgtccg gggcgagatc ctcaacatgg cctgcggcac ccgctacagc ctgaaccagc
     9901 tggtcgccct cgtggccggc ttgacgggga aagaggtggc tccgcaatat cgcgccgccc
     9961 gggcgggaga cgtccggcac tccgaggccg acatctcccg ggcccggcgg atcctcggct
    10021 acgagccccg ggtcagcttc gaagaggggc tccggcagac cgtcgagtac ttccagaaat
    10081 cggtggtccc gctcccgtag ccggggctac ccgaagtcag aacatccaca tcacgcggcg
    10141 cttgcgccgc gtcctcgaaa ggaacatcca ggtggcgaac cagccgtccg gccagcccat
    10201 ggtcggcatg atcgatttga aggcccagta cggccccctc cgtcctgaga tcgacgcggc
    10261 cgtgaagaac atcttcgata cggggcgctt catcctggga ccggaggtcg aggccttcga
    10321 gaaggagatc gccgagctcc acggcgtgcc gcacggcatc ggtgtggcca gcgggaccga
    10381 tgcgctcatg ctggtctggc gggcgctcga tctaaagccg gacgacgaag tggtcgtgcc
    10441 gtcgatgacc ttcctggcct cgggctcgag cgtgcggctg gccggcgcca caccggtctt
    10501 cgccgacgtc gatccgacca cgctcaacat gaccgccgcc acggcgcgtg ccgccgtcac
    10561 gaagaagacg aaggccatcc tggtcgtgca cctctacggc cagtgcgcca acgtccctgc
    10621 gcttgcggcg ctggccgacg aaatcggcgt gccgctcatc gaggattgcg cccagtcgat
    10681 cggcgccacc tgggacggcg tgccgtgcgg ctcgatcggc ctggcgggca ccacgtcgtt
    10741 ctatccctcc aagaacctgg gcgcgcccgg ggaagggggg atggtctgga gccgcgacga
    10801 ggcgttcgcc gccagggtcc gccgcctccg taaccacggg gaacgcgtcc ggtacgagca
    10861 tgtcgatctc ggcaccaaca gccggctgtc ggccatcaat ggcgcggtgc tccgcatcaa
    10921 gctgcgggcc ctgccgaagt ggaccgagct gcgtcgcgaa aacgccgcgt tctacgatgc
    10981 ggccttcgcc aacctggggc cgaccattcg cctgatgacg gtcgatcccc gcgcgaagca
    11041 cgtctaccac caatacagct tcttctcgcc cgagcgcgac aagctccgcg agcacctgac
    11101 ggcggccggg atcgagaaca tcgtgtatta ccccatcccg ctccatcgcc agatctgctt
    11161 cgcggatctc aaggccgatc ccgcgaactt cccgaacacc gagatggcgt gccgcgaggt
    11221 gctgtcggtg ccggtgcact ccgagttgac cgccgcgcag cgccaacggg tggccgatgc
    11281 cgtcgtgtcg ttcgcgaagg ccggcgccac gacgggaggg cgctgagatg gcccgccgca
    11341 tcctcgtcac cggcggggcc ggcttcatcg gcagccacgt ggccgaacag tacgcgctcg
    11401 acggctggca ggtccgcgtc ctcgacaacc tctcgcgcgc gaagcttctc aagaagtccg
    11461 acaggaacgc ctcattcaac tgggatcacc tctcgaagtt gccgggcgtc gagctgatca
    11521 aggggagcgt gaccgactgg gaaaccgtcc gccaggcggc ggccgattgc caggtgatcg
    11581 tgcacgcggc ggcccagacc gccgtcacga cctcggtcac cgacccgcga cccgacttcg
    11641 agaacaacat cgtcggcacg ttcaacgtgc tcgaggcggc ccgcgagcag tcggtgaagc
    11701 cggcgatcgt cttctgctcc accaacaagg tgtacggcga aaacgtgaac ggcgtcgggc
    11761 tctcggaagg ggagaagcgc tggtcgttcg agtcgtcgat ggcccgcggc atccccgaga
    11821 ccttcccgat cgaccactgc gagcacacgc cgtacggcgt gtcgaagctc acgggcgatc
    11881 tctacatgca ggactacgcg cacctctacg gcctcaagat cggcgtcttc cggatgtcgt
    11941 gcatctacgg cacgcgccag ttcggcctgg aagaccaggg gtgggtggcg tggttcacga
    12001 tcgcgtcgat gctcggcaag ccgatcacca tcttcggcga cggcaagcag gtccgcgacg
    12061 tcctctacgt cgaagacctg gtgggcgcct accgggcgtt cctcgattcc tcgctgaacc
    12121 acgcggtctt caacatgggg ggtgggccgg agttcacgat gtcgctgcac gagctgctcg
    12181 acatcctgaa ggccaggacg gggatcacgc cggcgctctc gtacgccgat tggcgcccga
    12241 gtgaccagaa ggtctacatc tcgaatatcg acaaggccac aaaggcgctg gggtggaagc
    12301 cctccgtcac gccggaacag ggcgtggcga agctgatcga ctgggtgaag gagaacaagg
    12361 gcctgtttgc ctgacgggtc cgggggcggt cctgcccggg gcggccgccg ggttcgggat
    12421 gtcgaagagc gaatggaaac gccatgagtc ctttcggacc catggcgttt tgatttcggg
    12481 cgtgggactc gtctgagggg cgtcgctact cgatgatcag cgacttgatc cggccccagg
    12541 tggaggagcg ggtcggcacg atcggttcgc aactggcccg cacgttgtcg aagcaggcgg
    12601 cccagccgtt gttgccggcg gagacctcga gctcgagcga gatgacgtgg ttgccgcacg
    12661 gaacggtgac cgtgacattg gtccagccga ccggccccag tgaccaggtg tactcgacgg
    12721 tgccatcgat ccgcaccacc acccggccgc tcagctggct cgcatcgagc tggtcgagcc
    12781 ggtaatcgag cgtgatgacg cagttgccgg gaatgccggg ctcgccgcag aggaaggtct
    12841 ggaagatcga gccggtgccg ccggagttgc cgaagggtga ctggatgcgt ccgtaggcat
    12901 ccgggttgcc gccggcggcc gggaacgaaa cggtccagtt ggccggctgg ttgacgttcc
    12961 agcccacgtt tccggccgag aagtcgccgt tgatgatgcc gagcggtggc gcggccgctg
    13021 tcagggtgac gagcgtggcg agcaggggaa gggcaaggaa cggacgccag gatttcatgg
    13081 gacctcctcg gatgggggac atccttgggc cggtccggcg cggccagccc ggaaacccgg
    13141 atcgtacgcc cgcggccggg ccgatccaca actatttcag cgcggcggcc tggcgcggcg
    13201 tgagccaggc ggcgggatcc tttccgtcgg ccaacgcgga attgatacgg gcggcctgca
    13261 gcggcgtgag gtcgggtgcc ttttcccagc gggtgccgga gaggtggcgc ttctggtccg
    13321 acggcggcgc ctcgcggaaa ccggggtctc cgtcccgcca ggccttgccg gccacgccgc
    13381 gccggtcggc ctcggcgatc agcgtggcgg cgtccaggtg ccccgcgtcg tacgttacgt
    13441 cgacgacttc ctggccgtcg atgaaaccgg cccgggtggc cagcaccccc tcgatcgagc
    13501 cgagccgcgc ttcgccttcc cagaagcagt gcatcgccag gtagagatgc cgcgcatcgt
    13561 ccacgtcgag ctcgtcggcc acgacgtgaa gccactcggg cgccgcatgc cccgaggcgt
    13621 cgagggccgc caccatccgc cgcgccacct cggcctccga ccagacacca tccttccgcg
    13681 cgatccagtc cttgccgtcg gcggccatga agcggacgac ggggttgttc caggccggtt
    13741 cgccgaagcg ctcgagcacg acctggtcgg cgcccccctt gttgttgtgg atggcgagcg
    13801 gcacgaagag cgtctcgatc ccctcgacca gccggggatt ggagaggacg gtctgcccga
    13861 agccgacgca ggtagcgcac cccgggatct cctggaagag cacgaagagg ggccgcccct
    13921 cggcgcgcga gtgggccgcc gcctcgttcc agtcgcgcag ccacgacacg cgcccgagct
    13981 cgcgggggtt tccgccggcg gccagcgtgg cggggatcac gactgcaacc agcgtggtga
    14041 ggagaaacgt caggacccgc tgaattgcca tttcaacttc ccccacgtcg tggtgcgagt
    14101 cgggctggac ggcgtcacga tcgccgtcac gtcatcgaaa taggcttccc aaccttcgat
    14161 gccgccgatg tcgcggcgaa gggacggcgc gccgtcgagg tcggggccgt ccaggcggta
    14221 gaggcacagc gccagggtgt gcgtcccggc cgggacggtt accgcagccg tacgccaacc
    14281 ggcctgaatt tcttcgggat cggtcgtcag gatcgtcgtg ccgtcgagcc ggatcgtcac
    14341 gccgccggtc cggggagcgg cgccgaggtt ggcatgacgg taggtgaccg agatgaggca
    14401 gacggcgtcg ctcccgggct cgccgcacga gaagagctgc gagaggcagc cgtagtcctc
    14461 gccgtccggc gtcgggctgg cgatgcgggc gcagccgccg gtcacgcccc ccgagtcggg
    14521 aaaggtggcc gtccaaccgc cgaccgggag gtcgtcgatc cagcccgctt cgtccgggtc
    14581 ctcgaaggcg ccattcacga cctggccggc ggccatcggg ggtgtcacga gcgccaggac
    14641 cagcgccagg aacgcgattc ggagcgtcat ccggccgatg ttactcctcg tcgggaccgg
    14701 gtgtcctcgc tcgcgccaaa tcacccgatc tgcgagaggg ggattgcagg ctgcacgaca
    14761 gcgcgactcc ccccggaagc ggcccgtccg gcggctggaa tgcgccaacc ggtaactggt
    14821 ttcgacacag gaagatggag ctgccacttc acgtgatcgg cgatctcccg atcgctggtc
    14881 catccggccc agcccttgct gtagccggcc ttggtatgga gacgactgcc ttggctacgg
    14941 cccgacgctt tccggaagac ccgctcgagc ggcgcctgga tgaaatccag gggctgctgc
    15001 gggagccgct cattcccgac gatgccgacc tgtccagccc cgccaccctt tccgacctgg
    15061 cgctgatcct ggccgaccgg gtggagcgcg gccaccgcca gctcattgaa acgagcgtcc
    15121 agctcaccag cctgcgcgag gtggcccatc gcctcctgtc ggtccgctcg gcagatcgcg
    15181 cggccgagac gatcgcgctc tacctgcaca aggcgttcga tcatggccgt gtggccgtcc
    15241 tcctgttcga ccgggaggcc ctcaccctcc gtggcaccgc catccgtcgg gcggggctcg
    15301 gcttcttcca ggcccaggtg gcgcttccga tcgcgggggc ccagggggcc atcccggcca
    15361 ctttcgcgtc gggcgaggcg cggctgatcg actcaccctc ggaagcgaac ctgtatgacg
    15421 gtgagggtga ccttgccggc gcctttgaca tggaggacct gggttccatt gtggtggcac
    15481 cactcaaggc ggcccacctc ttcggctcgc ccgtctcgaa cggcggctgg cgggcggggg
    15541 aggaggcggc gaacccgctc gatccgcgtc acacgatcct cggcctcatc ctcgtcggcc
    15601 gctcgcggcg cgaatcgccc ctgaccagcg acgacctggc tggaatcgaa tcagtagcca
    15661 ccacgctctc caccgtcgtc gagaacggcc gcctctacca ggaccttcgc cagggggaga
    15721 tgttccgcga cgacctcctg aactcgatgg ggtcggggct cgtggccatc aacctggcgc
    15781 gacgggccat ctcgatgaac cagacggccg tcgggctcac cggctgggag atctcggacg
    15841 tcctcggcga tgtgccgccg ttcctcccca tcgccgaccg gggcatggac gccgccatcc
    15901 gcggcgccag ctacgggaag ccggtgctcc ggaaggaagt ggtgctctcc cgcaaggacg
    15961 gcacgttctt cccggcgtcc gtctccacct cgccgctccg gaacgaggac ggcgagatct
    16021 acggcgtcat cgccacgttc caggacctca cggtcttcaa ggagatggag gagcggatcc
    16081 gccagctcga ccggctggcc gccctcggtc gcttcacggc cgccatcgcc catgagatac
    16141 gcaatccact ggccggcatt tcggccggcg tggaatacct cacgaaaggg atgtcgcagg
    16201 ccggcgatgc cgccgacaac atccgcttca tccagaccga gatcaagcgc ctcaaccaga
    16261 tcatcgagga cctcttccgg gtgacgcacc cgaggcccct catgacctcg cccgagcgcc
    16321 ccgaggcgat cctcgagcgc tcgctccgct gcctcggcga aatggccaac gagcggaaga
    16381 tcgaggtggt ccgcatcctg cccgactcgg tgccggccgt tccggtcgat gccgaccaga
    16441 tccagcaggt ggccatcaac ctgatcaaga acgccatcga ggcaatggag ccgggcggca
    16501 cgctcacgat gcgcaccggc actcgccccg gccgccccgg cgaggagccg tggatcacgc
    16561 tgtcggtttc cgacacgggc ccggggatga gcgccgaggt gcggaagaga ttgttcgagc
    16621 cgtttttcac gaagaaggc
//
LOCUS       DASVOD010000197         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_638320, whole genome shotgun sequence.
ACCESSION   DASVOD010000197 DASVOD010000000
VERSION     DASVOD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638320"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1289)
                     /locus_tag="VF720_04180"
     CDS             complement(108..1289)
                     /locus_tag="VF720_04180"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX7878578.1"
                     /translation="MSRLDFIDAELERLKSEGLYNQIRTIGSAQGAWIEIDGRRVLNL
                     CSNNYLGLANDPRLKKRAAEAIEKWGVGPGAVRSIAGTTSLHDELERKLSAFKKREAT
                     LSVQSGLLANVATIPALMESPEDALFTDELNHASIIDGVRLSKAQRHIYRHNDAGHLR
                     EQLKAATSVRRKLIITDGVFSMDGDVAPLDDLAKVAAEFDAMLMVDDAHGEGVLGSHG
                     RGAADHFGLHEEVDIEIGTLSKAFGVMGGFVSGSAKLVEFLKQKARPFLFSSAVTPAD
                     VAACIAAVEVLEESDAAVKKLWANGDYLKKGLREAGFDTGVSTTPITPVMLGEAERAR
                     AFSRKLFDKGIFAMAIGFPTVAKGKARIRCMVSAAHETADLDTAIAAFREVGKELGVA
                     G"
     gene            1399..2400
                     /locus_tag="VF720_04185"
     CDS             1399..2400
                     /locus_tag="VF720_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7878579.1"
                     /translation="MSDDFRPAFGMANPHVQTIWGRLTRSGPRMAVRREFLPTPDGDE
                     IVLDHLEFEGSAAPRTLILHGLEGSSQSVYAQGFLKLFAARRLNVTAMNFRSCARDPK
                     NNGKWIPNRGDRLYHSGETSDIGFVIDTMTKRDGRPLNIIGASIGGNVTLKWLGENPG
                     DPRVNRAAALSVPVDLAACADHLEKGRMGPFYTSIFLSTLKPKVAELAARSPKVAELF
                     DMKRALRARTFREFDEAATARIHGFRNAADYYARASSLPFLERIATPTLLVNSADDPF
                     IPPAVSAHLRGFNNGSGRAFRAEITSSGGHVGFIAGSIWKPRFWVEERLVNWVAEGA"
     gene            2515..3009
                     /locus_tag="VF720_04190"
     CDS             2515..3009
                     /locus_tag="VF720_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX7878580.1"
                     /translation="MSHLGPGAVAPEFSLPRLGGGLIQLDELLAVGPTWLFFLKSNCP
                     TCKLTAPYLQRFHEELGDRPGAIVAIMEDEPEAAAAFAATYGWTLPVGIEESPWPVSS
                     SYRLFSVPTNFVIGTHGIMTRVGAGFHRTELNETARELLHGAVNREYELIRAGDGAPE
                     FKPG"
     gene            3068..4468
                     /gene="lpdA"
                     /locus_tag="VF720_04195"
     CDS             3068..4468
                     /gene="lpdA"
                     /locus_tag="VF720_04195"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008990652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX7878581.1"
                     /translation="MAERKEYDIVVLGGGPGGYVAAIRAAQLGFKTAVVEKERLGGVC
                     LNWGCIPSKALLKNSEVYRSATKAAEWGITIEGLSFDFKKVIDKSRTVSDTIVKGVEF
                     LMKKNKIEVIPGTGKFISKNELEVTGSAATTRVAGKHIIIATGGRPRPLPFAPFDGKL
                     VLSSRHALDRREFPKSIVIIGAGAIGVEFASFYNAFGSKVTLVEMMPTLVPVEDKEVG
                     AILKKSFEKRGMQVFVGARVNSLKPGGAGIQAEVEAGGTKQVVEADCCLVAIGIVPNS
                     EEMGLDKIGVTVEKGFIPVDRKTFATNVPGVYAIGDINGPPLLAHVASAEGVVCVEQI
                     AGHKASKVDYDNIPGCTYCQPQIGSVGLTEEQCIEKKLDYKVGKFPYTALGKAKAVGE
                     TEGMVKLIFSKKYGELLGGHIVGPEATEQLAELNVLKGAEGTQVELHRTIHAHPTFSE
                     AVMEAAAAADGEAVHI"
     gene            4606..4887
                     /locus_tag="VF720_04200"
     CDS             4606..4887
                     /locus_tag="VF720_04200"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX7878582.1"
                     /translation="MPNDLDFEWDPGKSRQNELKHGISFELARLIWIDDMRVEIQACS
                     LEEPRHVVIGRIGSRLFAAVITYRGARIRLISVRRARGREVIHHESQEI"
     gene            4868..5101
                     /locus_tag="VF720_04205"
     CDS             4868..5101
                     /locus_tag="VF720_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX7878583.1"
                     /translation="MKAKKFDKLFDSGKDITPHLDLKSAKRPGQTIQRVNVDFPTWVV
                     ESLDREAQRLGVTRQSVIKMWIAERLDKSPKAS"
BASE COUNT          940 a         1714 c         1625 g          883 t
ORIGIN      
        1 ccgcaagggt atcgaccggc gcgccgagcg aatcgggcgc ggccgcgcgc gccgccgccc
       61 cggcaacgtt tacgagcagc aagacagcca gcgcgaacgc cgctgcctca acccgcaacg
      121 cccagctcct tacccacctc ccgaaacgcg gcgatggccg tatccaggtc ggccgtctcg
      181 tgcgccgccg acaccatgca gcggatgcgc gccttccctt tcgccaccgt cgggaaaccg
      241 atggccatcg cgaagatccc cttgtcgaac agcttgcggc tgaaggcccg cgcccgctcg
      301 gcctcgccga gcatgacggg ggtgatcggc gtggtggaga cgcccgtatc gaagcccgcc
      361 tcgcgcaacc ccttcttcag gtaatcgcca ttggcccata gcttcttcac ggccgcgtca
      421 ctctcttcca ggacttcgac cgccgcgatg caggccgcca catcggccgg cgtgaccgca
      481 ctcgaaaaga gaaacggtcg cgccttctgc ttcaggaact cgaccagttt cgccgacccc
      541 gacacgaacc cacccatcac gccgaacgcc ttggagagcg tgccgatctc gatgtccact
      601 tcctcgtgca acccgaagtg gtcggccgcc ccgcgcccgt ggctgcccag caccccttcg
      661 ccatgcgcgt cgtccaccat cagcatggcg tcgaactcgg ccgccacctt cgccaggtcg
      721 tcgagcggcg ccacgtcgcc gtccatcgag aagacaccgt cggtgatgat gagcttccgc
      781 cggacgctgg tggccgcctt gagctgttcg cgcaggtgcc ccgcgtcgtt gtgccggtag
      841 atgtgtcgct gcgccttcga gagccgcaca ccgtcgatga tcgacgcatg attcagctcg
      901 tcggtgaaca gcgcatcctc gggcgactcc atcagcgccg ggatcgtggc cacgttggcc
      961 aggagccccg actgcaccga cagcgtggct tcgcgcttct tgaacgcgga gagcttccgc
     1021 tccagctcgt cgtgcagcga ggtcgtcccg gcgatggacc gcaccgcccc cggccccact
     1081 ccccatttct cgatcgcctc ggccgcccgc ttcttcagcc gcggatcatt cgccaggccg
     1141 aggtaattgt tcgagcagag gttcaacacc cgccgcccgt cgatctcgat ccacgccccc
     1201 tgggcgctgc cgatggtgcg aatctggttg tagagccctt cggacttgag gcgttcgagc
     1261 tcggcatcga tgaaatcgag acgggacatg gggttcctcc ggagacggga tttgtaaccg
     1321 cgattttact atggctgcgg gccgtccggg aggagtccgc ccgcggctca gccgcatgac
     1381 atgcggtagc atctccgcat gtccgacgac ttccgccccg ccttcggcat ggccaacccc
     1441 cacgtccaga cgatctgggg gcggctgacg cgctcgggcc cacgcatggc cgtccgccgc
     1501 gagttcctcc ccacccccga cggcgacgag atcgtgctcg atcatctcga gttcgaaggt
     1561 tccgccgccc cgcgcacgct gatcctgcac ggcctcgaag ggagctcgca gtccgtctac
     1621 gcgcaaggct tcctcaaact ctttgcggcg cggcggctca acgtcaccgc catgaacttc
     1681 cgctcctgcg cccgcgaccc gaagaacaac ggcaagtgga ttcccaaccg cggcgatcgt
     1741 ctctaccact ccggcgagac cagtgacatc ggtttcgtga tcgacacgat gacgaaacgc
     1801 gacgggcggc ccctcaacat cattggcgcc tcgatcggcg ggaacgtcac gctcaagtgg
     1861 ctcggggaga accccggcga tccgcgcgtg aaccgtgcgg ccgcgctttc cgtgccggtc
     1921 gacctggcgg cctgcgcgga ccatctcgag aaagggcgca tggggccgtt ctatacgtcg
     1981 atctttctgt cgacgctcaa gccgaaagtc gcggagctgg cggctcgatc accaaaggtc
     2041 gccgagctgt ttgatatgaa gcgggcgctt cgcgcccgca cctttcgaga attcgacgag
     2101 gcggccacgg cacgcatcca tggattccgc aatgcggcgg actattacgc gcgggccagt
     2161 tcgctcccct tcctggagcg gatcgccaca ccgaccctgc tggtgaattc ggcggatgat
     2221 cccttcattc cgccggctgt gtccgcgcac ctgcgaggct tcaacaatgg ctccgggcgc
     2281 gcattccgcg ccgaaatcac gtcatccggc ggccacgtcg gcttcatcgc cggatcgatc
     2341 tggaaacccc gtttctgggt ggaggagcgc ctggtgaatt gggtggcgga gggcgcctga
     2401 ggcgccattg acccaccccg cccggtacct taattttccg ctacaggccc cgtccccgac
     2461 cggtggcggg gccttcgaat tggccgtttt caccccccgt cgaggtcccc cggcatgtcg
     2521 caccttggtc ccggcgccgt tgcgcccgag ttttcgctgc cgcggttggg cggggggctc
     2581 atccagctgg atgaactgct ggcggtgggg ccgacgtggc tcttcttcct caagtccaat
     2641 tgcccgacgt gcaagctgac tgcgccgtat ctgcaacggt tccatgagga actgggcgat
     2701 cggccgggtg cgatcgtggc catcatggaa gacgagccgg aggcggcggc ggccttcgcg
     2761 gccacgtacg ggtggacgct gccggtgggg atcgaggaat cgccctggcc ggtttccagc
     2821 tcctacaggc tcttcagcgt gccgacgaac ttcgtgatcg gcacgcacgg catcatgacg
     2881 cgcgtcggcg cgggttttca tcgcacggaa ttgaacgaga cggcgcgcga gttgctgcac
     2941 ggcgccgtga atcgcgaata cgaactgata cgcgccggtg acggcgcgcc cgagttcaaa
     3001 cccggctgag gcagccggaa cggccactcg tgaagtggcc ggtcaacgga acgagaggaa
     3061 cgagtcgatg gcggagcgca aggagtacga cattgtggtg ctgggcggcg gccccggtgg
     3121 ttacgtggct gccatccgcg ccgcgcagct cggtttcaag acggcggtgg tcgagaagga
     3181 acggctcggc ggcgtctgcc tgaactgggg ctgcatcccg agcaaggcgc tcctcaagaa
     3241 ctccgaggtg tatcgctcgg ccaccaaggc cgccgagtgg ggcatcacga tcgaagggct
     3301 gtcgttcgac ttcaagaagg tcatcgacaa gagccgtacg gtttccgaca cgatcgtgaa
     3361 gggcgtcgag ttcctgatga agaagaacaa gatcgaggtg atccccggca ccgggaaatt
     3421 catctcgaag aatgagctcg aggtgaccgg ctccgccgcc acgacgaggg tggccggcaa
     3481 gcacatcatc atcgccaccg gtggtcgccc tcgcccgctg ccgttcgcgc cgttcgacgg
     3541 caagctcgtg ctgtcgagcc gccacgcgct cgaccgtcgc gaattcccca agtcgatcgt
     3601 catcatcggc gccggcgcca tcggcgtgga gttcgccagc ttctacaatg ccttcggctc
     3661 gaaggtcacg ctcgtcgaga tgatgccgac gctcgtgccg gtggaggaca aggaagtcgg
     3721 cgccatcctc aagaagtcgt tcgagaagcg cggcatgcag gtgttcgtgg gcgccagggt
     3781 caactcattg aagccgggcg gcgccggcat ccaggccgaa gtggaagccg gggggacaaa
     3841 gcaggtggtg gaagccgact gctgcctcgt ggccatcggc atcgtgccga actcggagga
     3901 gatggggctc gacaagatcg gtgtcaccgt cgagaagggg ttcatccccg tggatcgcaa
     3961 gacgttcgcc accaacgtcc ccggcgtcta cgccatcggc gacatcaacg gcccgccgct
     4021 cctcgcgcac gtggcctccg ccgagggagt cgtctgcgtg gagcagatcg ccggccacaa
     4081 ggcctcgaag gtcgattacg acaacatccc cggctgcacc tactgccagc cccagatcgg
     4141 ctcggtgggc ctgaccgaag agcagtgcat cgagaagaag ctcgactaca aggtgggcaa
     4201 gttcccctac accgcgctcg gcaaggccaa ggccgtcggc gagaccgaag gaatggtcaa
     4261 gctcatcttc tcgaagaagt acggcgagct gctcggcggc cacatcgtcg gacccgaagc
     4321 cacggaacag ctcgcggagc tcaacgtgct caaaggcgcc gagggaacgc aggtggaact
     4381 gcatcgcacg atccatgcgc accccacgtt cagcgaggcc gtcatggaag cggcggcggc
     4441 ggcggatggg gaggccgtgc acatctaagg tggggccttt cgacgtgcaa tggacgccgt
     4501 gatcgacaat cctttccgat agccggcatg aagtgtccgg aagcatggaa attgccttga
     4561 caccatgtca tttaaaaaat atacctttat tataccgtcc ggaaggtgcc gaatgacttg
     4621 gatttcgaat gggaccccgg gaagagcagg cagaacgagc tgaaacacgg gatcagcttt
     4681 gagcttgcgc gattgatctg gatcgacgac atgcgcgtcg aaatccaggc atgttcactc
     4741 gaggagccga gacacgtcgt aatcggacga atcggctcaa ggctcttcgc agcagtcatt
     4801 acctaccgtg gcgcacggat tcgactgatc tcggtgcgac gtgcccgtgg ccgtgaggtg
     4861 atccaccatg aaagccaaga aatttgacaa gctctttgat tccggcaagg acatcacccc
     4921 acacctggac ttgaagagcg cgaagcgccc cggccagaca attcagcggg tcaatgtcga
     4981 ctttccgacg tgggtggtcg agtcactgga tcgtgaggct cagcgcctcg gcgtgacgcg
     5041 ccaatcggtc atcaagatgt ggatcgccga gcgcctggac aaatcgccga aggcgtccta
     5101 gtaccgtccc aggtccgcgc cgatcagaac cggaccgtcg tagccgccct tgcgcacacc
     5161 ct
//
LOCUS       DASVOD010000198         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1004504, whole genome shotgun sequence.
ACCESSION   DASVOD010000198 DASVOD010000000
VERSION     DASVOD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004504"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /locus_tag="VF720_04210"
     CDS             <1..2167
                     /locus_tag="VF720_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7878584.1"
                     /translation="YDSSEYIRESVSEVGETLMIAVGLVVLVILFFLRSFRATIIPTL
                     AIPTSIIGTFAVAYFLGFTINILTLLALVLAIGLVVDDAIVVLENIYRHMEMGKSRWR
                     AAIDGSHEIGFAVISTTIALVAVFVPVAFLQGSVGRLFNEFGTCVAASVAISGIVALT
                     FTPMLSSRILAPHSAPKSGIGKALDAFFRGMDSAYAATINFAMRNRLAVLVVALLLMI
                     GSVFVFRMLPRELVPTEDRGTAFGIVLAPEGATLEYTDRYMRQAEAILLNVPEREGLF
                     TATGLGFGGVGRVTNGFIFLNLKDRKDREKSQQQIVQELFPQMLGIPGVLAFLINPPS
                     LGGGFSSSPVEYILLGDSYEQLQGGIRAMMAEGQKLGYLINMDSDLRLNKPELDIDID
                     RERAAQLGVSVTDIGGTLETLLGGRAVTNFKRGSKQYDVIVQMKPSDRATPDKIEQVY
                     VKGPNGMVQLANVVQVRESVTPKELNHFNRVRAATITANLAPGVTLGKALDDLDKIAR
                     TSLPPGIKTDLGGQSREFRESSNRLYFMFFLAIGFIFLVLAAQFESFIHPLTILLSVP
                     LAVVGAIFSLFVFGQSLNIYSQIGLIMLVGLVTKNAILIVEFANQLRLRGEGVVQATV
                     EASRIRLRPILMTSFATIFGILPIAIGIGAGGEARRPLGIAIVGGLVFSTMLTLVVVP
                     VVYTLMARFTRLKRIEDDEDAPAGQPASAGHPSPAHARQ"
     gene            2374..>4024
                     /locus_tag="VF720_04215"
     CDS             2374..>4024
                     /locus_tag="VF720_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7878585.1"
                     /translation="MPGPSRPLADPRPMTADDLWALARIGAPTPAPDGTFAVVPVTTY
                     DMSANKGRTRLWRVPADGGESLPLTASDVSSDQAAFSPDGKWLTFIRRRDGEKPQLYR
                     MALGGGEAEKLTDLPLGAADPKWFPDGTALVFVAPVIMPELVATVPPIPGAAPTAAAT
                     TAPPAATGTSSAPAGTPVEPPTLVTTRRLMAQRDAEPVKARVTEDRVFRYWDRWLTGG
                     EVPHLFAIDLATRSLSDLTPESTRWFDFMEPCGQYDLSPDGSELAFTADASPAPHQVM
                     NWDLFTLAVTRDDAGIRKGGAVTNITADNPGNDLKPRYAPDGDSILYGRDTDLFFYAD
                     RVRLARYDRRTKQHEVLTEAWDRSLAGWEWRPDGKSIWLTAEHEGRQAIFTMPPAVIT
                     MPELLVKGGVAAGLAAAKDGAAWFTHQDLSHPAELHRLAPDGPMTRLTRFNDDRLAGL
                     AMGKVEDRTFAGAGGAKVQMYLVYPPGFDASKKYPLVHVIHGGPHGISGDQFHPRWNA
                     QMFAAPGYVVAMVNFHGSTSWGQDFAASIVGDHPTKPFADIM"
BASE COUNT          634 a         1464 c         1250 g          676 t
ORIGIN      
        1 ctacgattcc tcggagtaca tccgcgagtc ggtgagcgaa gtgggcgaga cgctgatgat
       61 cgccgtcggg ctcgtcgtcc tcgtcatcct cttcttcctg cgcagcttcc gcgccaccat
      121 catcccgacg ctggccatcc ccacctcgat catcggcacc ttcgcggtgg cctatttcct
      181 cggcttcacg atcaacatcc tcaccctgct ggcgctcgtg ctggccatcg gactggtggt
      241 cgacgatgcc attgtcgtgc tcgagaacat ctaccggcac atggaaatgg ggaagagtcg
      301 atggcgcgcg gccatcgacg gctcgcacga gatcggcttc gccgtcatct ccacgaccat
      361 cgcgctcgtg gcggtgttcg tgccggtggc cttcctccag ggttcggtgg ggcgcctctt
      421 caacgagttc ggcacctgcg tggccgcctc ggtggccatc tcgggcatcg tggccctgac
      481 cttcacgccg atgctcagct cgcgcatcct ggcaccgcac tccgccccga agtcggggat
      541 cggcaaggcg ctggacgcgt tcttccgcgg catggacagc gcctacgcgg ccacgatcaa
      601 ctttgccatg cgtaaccgcc tggccgtgct ggtggtggcg ctcctcctga tgatcggctc
      661 ggtattcgtc ttccggatgc tgccgcgcga gctggtgccc accgaggacc gcggcaccgc
      721 cttcggcatc gtgctggcgc cggaaggagc cacgctcgag tacaccgacc gctacatgcg
      781 gcaggccgag gcaattttgc tcaacgtgcc ggagcgcgag ggactcttca ccgccaccgg
      841 cctcgggttc ggcggcgtgg ggcgtgtcac caacggcttc atcttcctga acctcaagga
      901 ccgcaaggat cgcgaaaaga gccagcagca gatcgtgcag gagctcttcc cgcagatgct
      961 cggcatcccc ggcgtgctgg ccttcctcat caacccgccc agcctcggtg gcggcttctc
     1021 gtcgagcccc gtcgaataca tcctcctggg agacagctac gagcagctcc agggcggcat
     1081 ccgggccatg atggccgagg gacagaagct cggttacctg atcaacatgg attcggacct
     1141 ccgcctcaac aagccggagc tcgacatcga catcgaccgc gagcgcgcgg cccagctcgg
     1201 cgtgtcggtc accgacatcg gcggcacgct cgagacgctg ctcggcggcc gcgcggtgac
     1261 caacttcaag cgcggttcca agcagtacga cgtgatcgtg cagatgaagc cgtcggaccg
     1321 ggccacgccg gacaagatcg agcaggtgta cgtgaagggt ccgaacggca tggtgcagct
     1381 ggccaacgtg gtgcaggtgc gggagtccgt gacgcccaag gaactgaacc acttcaaccg
     1441 cgtgcgcgcc gccacgatca cggccaacct ggcgcccggc gtcacgctcg gcaaggcgct
     1501 ggacgacctg gacaagatcg cccgcacctc gcttcccccc ggcatcaaga ccgacctggg
     1561 cggccagtct cgcgagttcc gcgagtcatc gaaccgcctc tacttcatgt tcttcctggc
     1621 gatcggcttc atcttcctgg tgctcgcggc acagttcgag agcttcatcc acccgctcac
     1681 aatcctgttg tcggtgccgc tggccgtcgt cggcgccatc ttctccctct tcgtcttcgg
     1741 gcagagcctc aacatctaca gccagatcgg cctgatcatg ctggtgggat tggtcacgaa
     1801 aaacgccatc ctgatcgtcg aattcgccaa ccagctccga ttgcgcggcg aaggggtggt
     1861 gcaggccacg gtggaagcct cgcgcatccg cctccgcccc atcctcatga ccagcttcgc
     1921 caccatcttc ggcatcctcc ccatcgccat cggcatcggc gccggcggcg aggcgcgtcg
     1981 tcccctcggc atcgccatcg tgggcggcct cgtcttctcg acgatgctta cgctggtcgt
     2041 cgttcccgtg gtgtacacgc tgatggcccg gttcacgcgg ctcaagcgga tcgaggacga
     2101 cgaggatgcc ccggccggcc agccggcgtc ggcggggcat ccgtccccgg cgcacgcccg
     2161 gcagtagtcg agccggcaaa gccggttcga ccgcatttgt ccttcgacat cgtcgggcgc
     2221 gtggttcccc ggagccgcgc gcccggcctg tttccgggac tccgacccaa ggtgccagtt
     2281 gcctccaccc ttgggctata cttccgcgcg ctccttctga cgccatcgaa gcacgattaa
     2341 cgataccggt acatccccgc ggaggccccc gccatgcccg gcccgtcacg gcccctggcc
     2401 gatcctcgtc ccatgaccgc cgacgacctc tgggccctgg cccgcatcgg cgcaccgaca
     2461 cccgcaccgg acggcacgtt tgccgtcgtg ccggtcacca cctacgacat gagcgccaac
     2521 aagggacgca cccgcctctg gcgcgtgccc gccgacggcg gcgagtcgtt gccgctcacc
     2581 gcgtccgacg tgtcgagcga ccaggcggcc ttctcgccgg atggaaagtg gctgaccttc
     2641 atccggcgcc gcgacggcga gaagccgcag ctctaccgga tggcgctcgg cggcggcgaa
     2701 gcggaaaagc tgaccgacct gccgctgggc gcggccgatc cgaagtggtt tcccgacggc
     2761 acggcgctgg tcttcgtggc gccggtgatc atgccggagc tggtggccac ggtgccgccg
     2821 atccccggcg cggctcccac ggctgccgcc accacggctc cgcccgccgc caccggcacc
     2881 tcttcggcgc cggccggcac ccccgtcgaa ccacccaccc tcgtcaccac ccgtcgcctc
     2941 atggctcagc gcgatgccga acccgtcaag gcgcgagtga cggaagaccg cgtcttccgt
     3001 tactgggatc gctggctgac cggcggcgag gtgccgcacc tcttcgccat cgacctcgcc
     3061 acgcgctcgc tctccgacct cacgcccgag tcgacgcgct ggttcgattt catggagccg
     3121 tgcgggcagt acgacctgtc gcccgacggc tcggaactgg ccttcacggc cgacgcgagc
     3181 ccggcgccgc accaggtgat gaactgggat ctcttcacgc tggccgtgac acgggatgac
     3241 gcggggatcc gcaagggcgg cgccgtcacg aacatcaccg ccgacaaccc gggcaacgac
     3301 ctgaaaccac ggtacgcgcc ggacggcgat tccatcctct acggccgcga caccgatctc
     3361 ttcttctacg ccgatcgcgt ccgcctggcc cgctacgacc gcaggacaaa gcaacacgag
     3421 gtgctcacgg aagcgtggga ccgctcgctg gccggctggg aatggcggcc cgacggaaag
     3481 tcgatctggc tgaccgccga acacgaaggg cgacaggcca tcttcacgat gcctccggcc
     3541 gtcatcacca tgccggagct gctggtgaag ggtggcgtgg ccgccggcct ggcggccgcc
     3601 aaggacggcg cggcctggtt cacgcaccag gacctctccc accccgctga gctccatcgc
     3661 ctggcgcccg atggtccgat gacccgcctc acccgcttca acgacgatcg cttggccggc
     3721 cttgccatgg gaaaagtgga ggaccgcacg tttgcgggtg ccggcggcgc caaagtgcag
     3781 atgtacctgg tgtacccgcc cggtttcgac gcgtcgaaga agtacccgct ggtgcacgtc
     3841 atccacggcg ggccgcacgg catttcgggc gaccagttcc atccgcgctg gaacgcccag
     3901 atgttcgccg cgccgggcta tgtggtggcc atggtcaact tccacggctc cacgtcgtgg
     3961 gggcaggatt ttgccgcctc catcgtgggc gaccatccga cgaagccgtt cgccgacatc
     4021 atgg
//
LOCUS       DASVOD010000199         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_325279, whole genome shotgun sequence.
ACCESSION   DASVOD010000199 DASVOD010000000
VERSION     DASVOD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325279"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1141
                     /locus_tag="VF720_04220"
     CDS             248..1141
                     /locus_tag="VF720_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878586.1"
                     /translation="MRRLRITTIMLFLSAGCLWAQESGATFTVYFDPAQIATVGEGTT
                     TDTVRCQGYEFVYTRDKLFTGGGSVPIGRPVRVAWPTGVEAQYVTAGPNPEKARITVR
                     RVDGDVFDVVSFTARLLANAGAGRAIEIVPMLNGEEPFNDPFYFDVSGYYGQEFSYDR
                     TPNYLGTTAPLVDFDTYHIGLTLDYALVALTLEDHLPVSGVGDSPVSEAGRIHMTPNP
                     ARSPVQIRLEVAGEIMAGIPVEIHAVTGALVRTLRLDGIGRAAWDLRDERGQPVGAGV
                     YFARATTLRGIMRTERLIVLR"
     gene            complement(1182..>1582)
                     /locus_tag="VF720_04225"
     CDS             complement(1182..>1582)
                     /locus_tag="VF720_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878587.1"
                     /translation="IYTIVYSATDASSNVAYDTVDVRVQEPIGDPAAAAHPIDARVTA
                     LTTVQTSPFTPQTMVHFSLATSEHVRISIYDVTGSLVRKFVDDTMPAGRHQARWDGTS
                     DRGTGVPSGIYFVRMMTGSYREVRKIVVQR"
BASE COUNT          278 a          487 c          533 g          284 t
ORIGIN      
        1 ctttcgccat ggtgccccgg gcaccatcga atttcccgga ttgctgacct acaatgccgg
       61 cgcctcgatg acttgaagct ccatgacttg aactttggat aaatgaggaa gccgcaattc
      121 ctcaacccgc cacctcttga aggaccgccg ggaatgtgac gaatgtcgtc cggatcgcac
      181 tttccctggc catcgtggag cctgacagcc actgtcgggt taccgtggtg gccggggagg
      241 aaatgcgatg agacgcctgc gaatcacgac gatcatgctg ttcctgagtg ccggttgtct
      301 gtgggctcaa gaatccggtg caacgttcac ggtctatttc gatcccgcgc agattgccac
      361 ggtgggagag ggaaccacga ccgacaccgt ccgctgccag ggatacgagt ttgtctacac
      421 ccgcgacaag ctgtttaccg gcggggggtc ggtaccgatc ggacggccgg tgcgggttgc
      481 atggccgacg ggggtcgaag cgcagtacgt gaccgcggga cccaacccgg agaaggccag
      541 gatcaccgtg cgccgggtcg atggcgacgt gttcgacgtc gtgtccttca cggcgcgact
      601 tctggcgaac gcgggtgcgg ggcgggccat cgaaatcgtt ccgatgctga acggcgagga
      661 accgttcaac gaccccttct acttcgatgt gtccggctac tatggccagg agttctcgta
      721 cgaccggacc ccgaactacc ttggaacgac ggcgcccctg gtcgacttcg acacgtatca
      781 catcggcctg acgctggatt atgcgctggt ggccctcacg ctcgaagacc atcttccggt
      841 ctccggggtc ggcgattcac cggtctccga agccggccga attcacatga cgccaaaccc
      901 ggcccggagc ccggtgcaga tccgcctgga agtcgccggc gagatcatgg ccgggatccc
      961 ggtcgagatc cacgcggtga ccggagcgct cgttcggaca ctgcggctgg acggaatcgg
     1021 ccgtgccgcc tgggatctgc gggacgaacg gggacaaccg gtgggggccg gggtctactt
     1081 cgcgcgggcc acgacgctgc ggggaatcat gaggacggaa cggctgatcg tccttcgatg
     1141 acgacgcggt gacaaggggg gagacgccgc ccgggttcga tctaccgctg caccacgatc
     1201 ttccgtacct cgcggtacga gcccgtcatc atccgcacga agtagattcc cgaagggacg
     1261 ccggttcccc ggtccgacgt gccgtcccag cgcgcctggt gccggcccgc cggcatcgtg
     1321 tcgtcgacga acttccgcac cagggatccg gtgacatcgt agatcgagat gcggacgtgc
     1381 tcgctggttg ccagcgaaaa gtgcaccatc gtctgcgggg tgaacggact ggtctgtacg
     1441 gtggtcagcg cggtgacgcg cgcgtcgatc gggtgggcgg cggccgccgg gtcgccgatg
     1501 ggctcctgca cgcgaacgtc gacggtgtcg taggcgacgt tgctcgaggc atcggtcgcc
     1561 gagtagacga tggtgtagat gc
//
LOCUS       DASVOD010000200         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_586566, whole genome shotgun sequence.
ACCESSION   DASVOD010000200 DASVOD010000000
VERSION     DASVOD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586566"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1639
                     /gene="recJ"
                     /locus_tag="VF720_04230"
     CDS             <1..1639
                     /gene="recJ"
                     /locus_tag="VF720_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEX7878588.1"
                     /translation="HGFPRVGAEMLLRRGITADSDLQQFFEPRLKSLHDPFRLPDMPA
                     AAERAWAAINRKESILIHGDYDVDGMVGTAFLIRMMGAMGARTSYFLPDRVRDGYGLG
                     PAGLEAAAERGATLIVCVDCGVTAVEAATAAAARGIDLIILDHHQPPPALPSALAVVD
                     ALRTDADYPFMGLCGVGVAAKFIQAMAMSRPGRLEPAVYVDALQLVALGTIADVVPLL
                     DENRTFVMHGLKALARTEYPGLAALKRLARLTGGPITAEQVAYQVAPRLNAAGRMGVP
                     SLGVELLLAGTIERGEFLARELDNLNLHRRESDQAVSAAARAMAGGYGELPPFIVLWS
                     DDWSAGVIGIAAARVAEEFRRPALLIGMNGDVGRGSARSYAGLDVGGAFARVADLLVA
                     HGGHRQAAGLSVRRENLEALRERLVALAAEEPGDGAPEPLNLEGELRPESVDAGLVAF
                     LDRLAPFGAGSPEPLFLLRGLSLAAPPRIVGTDHLKLTLSMNGRSMGAIGFGLGRHAG
                     RLRESRESIALAATPSPDDWRGAGAIQLKFKDAGVMP"
     gene            1636..>2999
                     /locus_tag="VF720_04235"
     CDS             1636..>2999
                     /locus_tag="VF720_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEX7878589.1"
                     /translation="MIPPGQAEAIHESLLEMVEARHLDVDRGALRHAFDYSFAAHGTQ
                     TRANGDPYMVHPVEVVKILIGLLDKRVDTTLLCAALLHDVAEDTDISIEDIGREFGKE
                     VAYLVDGVTKITGFEFASTESEQAENFRKMLMSMAHDVRVILIKLADRLHNMRTLDYL
                     AADKAQRIARETQEIYAPLAHRLGIGTIKWELEDLAFKYTDRAAYKDLVDKVAMSRAE
                     RERLIQAVQAPIQRELAAQGIRSEISGRPKHLYSIYRKMTSQGRPFNEIFDLLGVRVI
                     VPTKADCYRVLGVLHDLFTPVHERFKDYIATPKGNMYQSLHTTVIGPEHKMVEVQIRT
                     GDMHKTAELGIAAHYSYKEGLWPEEELSSKLGELFGESGFWAGNDATGDPGEFLDFLK
                     TSLYQDEVFVFTPKGELKRLPKGSTPLDLAYMIHTDIGHRTVGARVGGRIVSLKYELK
                     NGDSV"
BASE COUNT          482 a         1023 c         1028 g          466 t
ORIGIN      
        1 gcacggcttc ccgcgggtgg gggcggaaat gctcctccgc cgcggcatca cggctgactc
       61 cgacctccag cagttcttcg agccccgcct caagtcgctc cacgacccgt tccgactccc
      121 cgacatgccg gcggccgccg agcgcgcctg ggccgccatc aaccggaagg aatcgattct
      181 cattcacggc gactacgacg tggatggaat ggtcggcacc gctttcctca tacggatgat
      241 gggggcgatg ggggcgcgca ccagctactt tcttcccgat cgggtgcggg atggttatgg
      301 ccttgggccc gccgggctcg aggcggccgc cgagcggggg gccacgctca tcgtctgtgt
      361 cgactgcggc gtcacggcgg tggaagcggc gaccgccgcg gccgcgcgag ggattgacct
      421 catcatcctt gatcatcacc agccgcctcc ggcactccct tctgccctcg cggttgtgga
      481 tgcgctccgc accgacgccg actatccatt catggggctg tgcggcgtgg gcgtggcggc
      541 caagttcatc caggccatgg ccatgtcgcg gccggggcgg ctcgagcccg ccgtgtacgt
      601 ggacgcgctg cagctggtgg cgctcggcac catcgccgac gtcgtgccgc ttctcgacga
      661 gaaccgcacg ttcgtcatgc acgggctcaa ggcgctggcg cgcaccgaat atcccggcct
      721 cgcggcgttg aagcgcctgg cgcggctcac cggcgggccg atcaccgccg aacaggtggc
      781 ctaccaggtg gcgccccgcc tcaacgcggc cgggcggatg ggggtgccct cgctcggcgt
      841 cgaactcctg ctggccggca cgatcgagcg gggcgagttc ctcgcgcgcg agctcgacaa
      901 cctgaacctg caccgccgcg aatcggacca ggcggtgagc gcggccgccc gcgccatggc
      961 gggcggctac ggcgagctgc cccccttcat cgtgttgtgg tcggatgact ggtcggcggg
     1021 tgtcatcggc atcgcggccg cccgcgtggc cgaggagttc cgccggccgg cgctcctgat
     1081 cggcatgaat ggcgatgtgg ggcgcggctc ggcccgctcg tacgcggggc tggatgtcgg
     1141 cggcgccttc gcccgcgtgg ccgatctcct ggtggcccac ggcggtcatc ggcaggcggc
     1201 cgggctcagc gtgcgacgcg agaacctgga agcgctgcgg gagcgccttg tcgcgctggc
     1261 cgccgaggag ccgggcgatg gcgcacccga gccgctgaac ctcgaaggag agcttcgccc
     1321 cgaatcggtg gatgcgggcc tggtggcctt cctcgatcga ctcgcgccgt tcggcgccgg
     1381 cagccccgag ccgctcttcc ttctgcgcgg gctctcgctg gccgccccgc cgcgcatcgt
     1441 cggcacggac cacctgaagc tcaccctgtc gatgaacggg cgctcgatgg gagccatcgg
     1501 tttcgggctc ggtcgccacg cggggcggct ccgcgagtcg cgcgagtcga tcgccctggc
     1561 ggccacgcca tcgcccgatg actggcgcgg cgcgggcgcc atccagctca agttcaagga
     1621 tgccggggtg atgccatgat cccgccggga caggccgagg ccatccacga atcgctcctc
     1681 gaaatggtgg aagcacgcca cctggacgtg gaccgcggcg ccctccgcca cgccttcgac
     1741 tactcgttcg ccgcccacgg cacgcagacg cgcgccaacg gcgacccgta catggtgcat
     1801 ccggtcgagg tggtcaagat cctgatcggg ctcctcgaca agcgggtgga cacgacgctc
     1861 ttgtgcgccg cgctgctcca cgacgtggcg gaggacaccg acatctcgat cgaggacatc
     1921 ggtcgggaat tcggcaagga agtggcctac ctggtcgacg gcgtgaccaa gatcaccggc
     1981 ttcgaattcg cctccaccga gtcggagcag gccgagaact tccgcaagat gctgatgtcg
     2041 atggcgcacg acgtccgcgt catcctgatc aagctggccg accggctcca caacatgcgc
     2101 acgctcgact acctggcggc cgacaaggcc cagcgcatcg cccgcgagac gcaggaaatc
     2161 tacgcgccgc tggcgcaccg actcggcatt ggcaccatca agtgggaact ggaggacctg
     2221 gccttcaagt acaccgaccg ggccgcgtac aaggacctgg tggacaaggt ggccatgagc
     2281 cgggccgagc gcgagcggct gatccaggcc gtgcaggcgc cgatccagcg cgagctggcc
     2341 gcgcagggga tccggtccga gatctccggg cggccgaagc acctgtattc gatttaccgg
     2401 aagatgacga gccaggggcg cccgttcaac gagatcttcg acctgctcgg cgtgcgcgtg
     2461 atcgtgccga ccaaggccga ctgctaccgc gtgctgggcg tcctgcacga cctgttcacg
     2521 ccggtgcacg aacggttcaa ggattacatc gccacgccca aggggaacat gtaccagtcg
     2581 ctccacacga cggtcatcgg cccggagcac aagatggtgg aggtgcagat ccgcaccggc
     2641 gacatgcaca agaccgcgga actgggcatc gccgcccact actcgtacaa ggaagggttg
     2701 tggcccgagg aggagttgag ctcgaagctg ggcgaactgt tcggtgagtc gggcttctgg
     2761 gccggcaacg acgcgacggg ggaccccggc gaattcctcg acttcctgaa gacgtccctg
     2821 tatcaggatg aggtattcgt cttcacgccg aagggtgaat tgaaaagact ccccaaggga
     2881 tccacgccac tcgacctggc ctatatgatc catacggaca tcggacaccg gaccgtgggc
     2941 gctcgtgtcg gggggcggat cgtgtcgctc aagtacgaac tcaagaacgg cgactcggt
//
LOCUS       DASVOD010000201         3970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_13730, whole genome shotgun sequence.
ACCESSION   DASVOD010000201 DASVOD010000000
VERSION     DASVOD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13730"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="VF720_04240"
     CDS             complement(<1..1685)
                     /locus_tag="VF720_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX7878590.1"
                     /translation="MARARALRRQSRQEPGNDSLRYELALAEAEVGTIQERIRSLELF
                     ESLRATYGKEARYHRDRARVYEACQQASLAREALEDLLKLDPGDVAARVGVARLRLVE
                     LLYHFDLPETARMLEVLEPALALAPNDRGVLFYHSLALEMAAGLPDRESPRLSERGLA
                     EAERMAANDATDFEARLLAAVHAMDLGRSDRAARYFEAALAIAPEDVRSAFLTSRWTA
                     PPAAVRALESRDATDRAAYDTVYWRHHDPSPLTALNENQLEIWKRLALAEFLFARPAS
                     GVRGWDTEPGIAFVRYGAPRESHYETGDITATGPGPDRRLPYAGVGRGLNAPLRLQHP
                     SWEWVHHFRGLQFGLRFIDMNLNGEFTADNPTRKTLDFLHDSAPVVFHEAPPGPLRYL
                     AVAAAGFLAEERRVNENVYLGVPLWRPLGDGGWLGDVTMELIVRDTTRTIVRQARHRA
                     TPEDVVPMLGSDVSLLLLHQPWALVPGSYTITGYVEDADRKVHGVYTGPLTIRDYSKV
                     TGLVVSDLDLTLRPESRDSRVTVTRLGSSYIPNPLRVATDDRRIDIFYEVYGLT"
     gene            complement(1820..>3970)
                     /locus_tag="VF720_04245"
     CDS             complement(1820..>3970)
                     /locus_tag="VF720_04245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX7878591.1"
                     /translation="VAGVFLVTGSSNVENPAALARARALRKESGRDVRNDSLKYELAL
                     AEAEVGTIETRLRALTLFAEIRPAYEHETRFHRDRARVYESCSQPSSARECLEDLLKI
                     DPTDVNARVDASRLLLNELLYHYDLRLTWHMLDLLDPALAQAPDDRRVLFYHSLALEL
                     ASGLPEQTSPELSRRGLADAERLMGLDSTDVDACFLAAIHAMDLGQPDRAARRFEDAL
                     AIAPEDVRAAFLTSRWTAPVAAVAALKSRDEAGRASYDRAYWRHHDPSPFSSINENQL
                     EIWKRLALADFLFTRPGSDIHGWDTEPGLAFVRYGAPLIHGFDPGEVVASGPGIDKTL
                     PRYGWSSVATDHASINLIPPSWSWEHRFKGLEFDLRFVDRNLRGDFRADAGTTKVLEV
                     LREAQPVVFHEAPPGPLRYLAVTSAGVLDERESVVENVYLGVPLWRPLGDGKWLGDVT
                     MELIVRDTTRVIVRQARHKATPEDVVAILGQDVSGFLFNQPWRLPPGAYTVTGYVEDA
                     GKKLHGIYTGPLTVRDYLRPKGPVISDLDLTLRPATSDSRVAVTRLGSRYIPNPLRVA
                     TDDRQLDIFYEIYGLKEKSGTAFIETRYTILPRAWVEGFDRLVRAGDEQAGQMLETAE
                     SDADAEGSELSRSNYLQVTFPPAGLNLVNGRGAKGSRVPVPDLEPGEYDLVMTVRDIH
                     AGLSATASTWFQILSPEEREDLVAMGREAASRRP"
BASE COUNT          628 a         1282 c         1454 g          606 t
ORIGIN      
        1 gtgagtccgt acacctcgta gaagatgtcg atccggcgat cgtcggtggc cacgcggagc
       61 gggttcggga tgtagctcga accgaggcgg gtgacggtca cccgcgagtc gcgcgattcg
      121 gggcggagcg tcaggtcgag atcgctcacc acgaggccgg tgaccttgga gtagtcgcgg
      181 atggtcaggg ggccggtgta aaccccgtgc accttgcggt cggcatcttc cacgtaaccg
      241 gtgatggtgt agctgccggg aacgagcgcc catggctggt gaagaaggag gagcgacacg
      301 tcgctcccga gcatcggcac cacgtcttcg ggcgtggccc ggtggcgggc ctggcggacg
      361 atcgtacgcg tggtgtcgcg gacgatgagc tccatggtga cgtcacccaa ccagccgcca
      421 tcgcccagcg gccgccagag cggaacgccc aggtaaacgt tctcgttcac ccgcctttcc
      481 tcggccagga agccggcggc ggcaacggcc aggtagcgga gcggccccgg gggcgcttcg
      541 tgaaagacca ccggcgccga gtcgtgcagg aagtcgagcg tcttccgggt gggattgtcg
      601 gcggtgaact cgccgttcag gttcatgtcg atgaagcgaa ggccgaactg caacccccgg
      661 aagtgatgga cccactccca ggacgggtgt tgcagccgga gcggcgcgtt gagtccacga
      721 ccgactcccg cgtacgggag ccgacggtcg gggccgggcc cggtggcggt gatgtccccc
      781 gtttcgtagt ggctctcgcg cggggcgccg tatcggacga aggcgattcc cggctcggtg
      841 tcccagccgc ggacgccgga cgccgggcgc gcgaagagga attcggccag cgcgaggcgt
      901 ttccagattt ccagctggtt ctcgttgagc gcggtgagcg gcgagggatc gtgatgtcgc
      961 cagtacaccg tgtcgtaggc cgcccgatcg gtggcatcgc gcgattcgag ggcgcgaacg
     1021 gcggccggcg gcgcggtcca gcgcgaggtg agaaatgccg agcggacgtc ttccggcgcg
     1081 atcgccagcg ccgcttcgaa ataccgggcc gcccgatccg accgccccag gtccatggcg
     1141 tgcacggccg ccaggaggcg cgcctcgaaa tcggtggcgt cgttggccgc catccgctcg
     1201 gcttcggcca gaccccgctc gctcaacctt ggcgactccc ggtcgggaag gccggcggcc
     1261 atctccaggg ccagcgaatg ataaaaaagc acgccgcggt cgttcggagc cagggcgagc
     1321 gcgggctcga gcacctcaag catgcgggcg gtttcgggga ggtcgaagtg atagaggagc
     1381 tcgacgaggc ggaggcgcgc cacgcccacc cgcgcggcca cgtcgcccgg atcgagcttc
     1441 aggagatctt cgagcgcctc gcgagccagc gaggcctgct ggcaggcctc gtacacgcgc
     1501 gcacgatcgc ggtggtagcg ggcctccttt ccgtacgtcg cgcgaaggga ttcgaacagc
     1561 tcgagggatc gaatccgctc ctggatggtg ccgacctcgg cctcggccag ggccagctcg
     1621 taccggaggg aatcgttgcc gggctcctgc cgtgactggc ggcgaagcgc cctggcccgc
     1681 gccatccttg ccgggtcttc ggtgtttgtg ccagcgccga gaaggcaaac tgttgcgacg
     1741 acgagcaggg cgggcatcgc gagcctcacg agcgcaccgc ggcggcgccg tgcgcggaca
     1801 tcgccgccgc gcgcgccgtt cacggacggc gggatgcggc ttcgcgcccc atcgccacca
     1861 gatcctcccg ctcttcgggc gagaggatct ggaaccaggt gctggccgtg gcgctcaggc
     1921 cggcgtgaat gtcgcgaacg gtcatgacga ggtcgtactc ccccggttca agatcgggca
     1981 ccgggacgcg cgatcccttg gcgccgcggc cgttgaccag gttcaggccg gccggcggga
     2041 aggtcacctg gagatagttg ctgcggctca gctcgctccc ctccgcgtcg gcatccgact
     2101 cggccgtttc gagcatctgg ccggcctgct cgtcgcccgc gcgcaccagg cggtcgaatc
     2161 cctccaccca ggctcgcgga aggatggtgt agcgggtttc gatgaaggcc gtgcccgact
     2221 tttccttgag gccgtagatc tcgtagaaga tgtcgagctg gcgatcgtcg gtggccacgc
     2281 ggagcgggtt cgggatgtag cgcgagccga ggcgcgtgac ggccacgcgc gagtcgctgg
     2341 tggcgggacg gagcgtgagg tcgagatcgc tgatcaccgg gcccttcggc cggaggtagt
     2401 cacgtacggt caggggcccg gtgtagatgc cgtgcagctt cttccccgcg tcctcgacgt
     2461 aaccggtgac cgtgtacgcg cccggcggga ggcgccaggg ctggttgaag aggaagccgc
     2521 tgacgtcctg cccgaggatc gccacgacat cttcgggggt ggccttgtgc cgggcctgcc
     2581 gcacgatgac gcgggtggtg tcgcgcacga tgagctccat cgtcacgtcg cccagccact
     2641 tgccgtcacc cagcggccgc cagagcggca cgccgaggta cacgttctcg accacgctct
     2701 cccgttcatc cagcacgccg gcgctcgtca ccgccaggta gcgaagcggg ccgggaggcg
     2761 cctcgtggaa gacgaccggc tgggcctcgc gcagaacctc gagcaccttg gtggtgccgg
     2821 catcggcgcg gaaatcgccg cgcaggttcc gatcgacgaa gcggagatcg aactcgagcc
     2881 ccttgaagcg atgctcccag gaccacgaag gcggaatcag gttgatcgac gcatggtcgg
     2941 tagccacgct gctccagcca tatcgcggaa gtgtcttgtc gatccccggt ccggaagcca
     3001 ccacctcgcc cggatcgaat ccgtggatga gcggcgcccc gtagcgtacg aaggcgaggc
     3061 cgggttcggt gtcccagcca tggatatcgc tgccggggcg cgtgaagaga aagtccgcca
     3121 gggccaggcg cttccagatc tcgagctggt tctcgttgat ggaggagaag ggcgacgggt
     3181 cgtggtgccg ccagtaggcg cggtcgtagg aggcgcgacc ggcctcatcg cgtgacttca
     3241 gggcggccac ggccgcgacg ggagccgtcc agcgcgaggt gaggaaggcc gcgcggacat
     3301 cctcgggcgc gatggcgagc gcgtcctcga accggcgggc ggcccggtcg ggctgcccca
     3361 ggtccatggc gtggatggcg gccaggaaac aggcatcgac gtcggtggaa tcgagcccca
     3421 tgagccgctc ggcatcggcg aggccgcggc gactcaactc gggagacgtc tgttccggaa
     3481 gcccggaggc cagttccagg gccagcgaat gatagaagag cacccgtcgg tcgtccggcg
     3541 cctgcgccag cgccgggtcg agcaggtcga gcatgtgcca ggtgagtcgg agatcgtagt
     3601 ggtagaggag ctcgttcagg aggaggcgcg acgcgtcgac ccgcgcgttc acatcggtcg
     3661 gatcgatctt gagcaggtcc tcgaggcact cccgcgcgct cgacggctgc gagcacgact
     3721 cgtagacgcg ggcgcgatcg cgatgaaaac gggtctcgtg ctcgtaggcg ggccggatct
     3781 cggcaaagag ggtcagtgcg cggagccgcg tctcgatggt gccgacctcg gcttcggcca
     3841 gcgccagctc gtacttgagc gagtcgttcc tgacgtcgcg tcccgattcc ttgcgcagcg
     3901 ccctggcgcg cgcgagggca gccggattct cgacgttgct cgaacccgtg accaggaata
     3961 cgcccgccac
//
LOCUS       DASVOD010000202         8287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_221569, whole genome shotgun sequence.
ACCESSION   DASVOD010000202 DASVOD010000000
VERSION     DASVOD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221569"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VF720_04250"
     CDS             complement(<1..504)
                     /locus_tag="VF720_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7878592.1"
                     /translation="MPLQRYRSMRRFDETPEPRGARPGNSEKLSFVVQKHAASRLHYD
                     FRLEVGGVLVSWAVPKGPSTTPGEKRLAVHVEDHPLEYGSFEGVIPKGQYGAGTVEIW
                     DKGTWVPEGDPKRGIRAGNLKFQLKGRKLKGGWALVRMAPHDDEKGKENWLLIKHDDA
                     RGTGADAA"
     gene            complement(508..1446)
                     /locus_tag="VF720_04255"
     CDS             complement(508..1446)
                     /locus_tag="VF720_04255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX7878593.1"
                     /translation="MAHAVWKGTLEFGLVTIPIGLYPAESSEDLDLRMLDSEDLSPVG
                     YNLINKSTGESIPRSRVVKGYEYEEGRYVTLTDEELKSANAEATQTVDIVGFVDVEEI
                     PPSYFDRPYWLAPEKKGQKAYVLFREALLKARKIGVARVVLRTRQHMAAVYPQGDILV
                     LQLLRYASEFRDTKSLDIPDKAPRGHDVSPREVAMAIELIGQMAEPWKPAAYHDEYHD
                     QLLEYIESKAKTGKGKTIPAARASTGGGKVLDLMSLLKKSVEQGKRGSSPRTATATRS
                     ESSRAGGARSASSRRKAPAPLKLRATKKPAPKRRSA"
     gene            1725..3338
                     /locus_tag="VF720_04260"
     CDS             1725..3338
                     /locus_tag="VF720_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7878594.1"
                     /translation="MNRKACDAAGNRSLWALPMPEITVTGRNLDEDLTTDVCVVGGGI
                     AGLTSAYLALLDGASVVLIDSWDIAGGESCRTSAHLSNMLDRRYVAIESIHGEDGAFL
                     AAASHSAAIDRIERIARDESIDCRFARLDGYLFSPPGGSLDDLEKELTATRRAGLAVD
                     LIARAPLPDFDTGPCLRVGGQAHFDPTRYLAGLAAAIVRRGGRLYADSPAESVAGGAE
                     PVVTMANRRTIRAKAVIVATNSPMNDYVSIHLMQKAFRTYVVGGLVPGDTIARALFWD
                     TADPFHYVNVIPAAALVGRSVPDGHDLLLIGGEDHRTGRGDPTAERFDQLIEWAAARF
                     PGIGPFVVRWSGQVLESIDGLGLIGADPEKRANVYIATGDSGNGLTHGTIAGMLLTDL
                     IAGRSNPWRDLYSPRRKPVGALGNFVEENARMTYQYADWLHGSEVHDESSIAPGSGAV
                     LREGLKKWTAVHRRMDGTVVRRSAVCPHLGCIVHWNAAECSWDCPCHGSRFDAEGSLL
                     NGPALTGLAEAPDAREGAGADETAKPASG"
     gene            complement(3341..4213)
                     /locus_tag="VF720_04265"
     CDS             complement(3341..4213)
                     /locus_tag="VF720_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013628096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7878595.1"
                     /translation="MPAKTRKKRVARPAQRQSRQPGKQAPMRPQPVSVDDRYRGTGKL
                     AGKVALITGGDSGIGRAVAILFAREGARVAIVYKDEHADAKETARLVEREGARVLLLP
                     GDIGKEPFCRQAVARTVKELGALDILVNNAAEQHSVESPEAMTARQVEATFRTNVFGA
                     FHFIRHALPHLPKGGCIINTTSVTAYRGSPHLVDYAATKAALVGLTRSLAMALVERGI
                     RVNAVAPGPIWTPLIPATFRPKDVATFGSDVPMGRAGQPEEVAPSYVFLASDDASYIT
                     GQVLHPNGGEVVNA"
     gene            complement(4374..4829)
                     /locus_tag="VF720_04270"
     CDS             complement(4374..4829)
                     /locus_tag="VF720_04270"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7878596.1"
                     /translation="MKKMIWGAVASLALVALFAGMSQGGGKPFNVTLSGASEVPGPGD
                     PDGTGALKLTVNPGLGEICYTLTWDGLDGATAAHIHRAPIGEAGPVVVPLEVSDTGSS
                     NDCITIDKDLAREILQNPDMFYVNVHNETYPSGAIRAQLSGADDTEDEK"
     gene            5222..6466
                     /locus_tag="VF720_04275"
     CDS             5222..6466
                     /locus_tag="VF720_04275"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX7878597.1"
                     /translation="MDRPIDSAVMEGRKRRRWITLAGAAAVLVLLMALLPAWVRPAVE
                     RGRLRTGRVDRGPIEMTLSAAGTVVPEVERTIVAPGPSRVLRLLKQPGDSVAVGEPIL
                     ELDNGASRLTVERLTRQMAMKENEREQARVDQDNRLSELRTQSDIKSLELKSKQFDVE
                     RNTKLKEMGVISGDVLRQSQNDTERLAIEIAELTRRAANADRELAVRLKSLDLEYDIL
                     AKERDEAARVLDRGTASSDIAGVLTWVLGTEGTAVNAGDELARVADLDRFKVDATLSD
                     VYAARIRPGMPAFVAAGEARLPGRVSRVLPTVDVGTLRLEVALDDQSNAVLRHNLRVE
                     AHVIVDRREGALRVARGTFPTIDGNLCAFVVRGDRAVRRPIRLGLTGWEAAEILEGLN
                     EGDEVVLSDMSEYLNQKEIRIR"
     gene            6463..7191
                     /locus_tag="VF720_04280"
     CDS             6463..7191
                     /locus_tag="VF720_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878598.1"
                     /translation="MNAIATLERAADAIAGIRRHPAIRLVDVNKIYRTDRIETVALDG
                     VNLTVEPGEFISIMGPSGSGKSTLLHLVGLLDRPSTGTLEITGRPVMGLNDRALARLR
                     NETIGFVFQTFHLVNDLNVVDNVEIPLLYRKMSGSDRRRRAMEALARVGLSARTHHHP
                     GQLSGGQRQRVAIARAIAAEPRILLADEPTGNLDSQMGDEIMAILESLNREQGTTVVM
                     VTHDQRLAERTRRTIRLFDGRQVQ"
     gene            7200..>8287
                     /locus_tag="VF720_04285"
     CDS             7200..>8287
                     /locus_tag="VF720_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878599.1"
                     /translation="MFLSHLKIALKVLLRRKFFTFVSLFGTAFTLVVLMVIAAMMDGF
                     LSAGGPEPNLDRTLYISRMAMRGPENEWNGNPGYGFLDKYCRDLPGVQVMSLYTDAGN
                     TISFLDGNKITSDLRRTDAQYWTIHRYRFLEGGPFTEDDVTSARPVAVINATTRHRFF
                     GDGTAVGRTLETGGRSYTVVGVVADVSRARDAVYGDIWAPLTTAKTDDYRTRLMGSFS
                     AALLTRSRADFPAIRAEFARRLPTVQMSEPERYDRMEGAPRTRFEDMAGEIAGTEGPS
                     EKARTTLFLFGATLAALLFMSLPAINLVNLNISRILERSSEIGIRKAFGATSGHLVGQ
                     FLLENIVLVLMGGLIGLAASAWILDLINR"
BASE COUNT         1324 a         2743 c         2829 g         1391 t
ORIGIN      
        1 ggcggcgtcg gcgccggttc cgcgcgcgtc gtcgtgcttg atgaggagcc agttctcttt
       61 tcccttttcg tcgtcgtgag gggccatgcg gacgagcgcc catcccccct tgagcttcct
      121 tcccttcagc tggaacttga gattgccggc gcgaatgccg cgcttcggat caccctccgg
      181 aacccaggtt cccttgtccc agatctcgac ggtgccggcg ccatactgcc ccttcgggat
      241 gaccccttcg aagctgccgt actcgagggg atgatcctcg acatgcacgg ccaggcgttt
      301 ttcaccggga gtggtgctcg gccccttggg aacggcccag ctcaccagga cgccgccgac
      361 ttcgagacgg aagtcgtagt gaaggcgcga ggcggcatgc ttctgcacga cgaaggatag
      421 tttttcgctg ttgcccggcc gggcgccgcg cggctcgggc gtttcgtcga agcggcgcat
      481 ggagcggtag cgctggagcg gcatgactca ggcggagcgg cgcttcggcg ccggcttctt
      541 cgtggcacgc agcttgaggg gcgcgggagc cttcctgcgc gacgacgcgg agcgcgcccc
      601 accggcgcgt gagctctcgg accgggtggc ggtggcggtg cgtggagagg acccccgctt
      661 cccctgctcc acgctcttct tgaggagcga catcaggtcg agcaccttgc cgccgccggt
      721 cgaagcgcgg gcggcgggga tcgtctttcc cttgccggtt ttcgccttcg actcgatgta
      781 ttcgagcagc tggtcgtggt actcgtcgtg atacgcagcg ggcttccacg gctcggccat
      841 ctgcccgatc agctcgatgg ccatggccac ttccctgggg gacacgtcgt ggccgcgcgg
      901 cgccttgtcg gggatgtcga gcgacttcgt gtcgcggaac tcactggcgt agcggaggag
      961 ctggagaacc aggatgtcgc cctgcggata gacggccgcc atgtgctggc gcgtgcgcag
     1021 caccacgcgg gccacaccga tcttgcgcgc cttgagcagc gcctcacgga agaggacgta
     1081 ggccttctgc cctttttttt cgggcgccag ccagtaggga cggtcgaagt acgacggcgg
     1141 aatttcctcg acgtcgacga agccgacgat gtcgacggtc tgcgtggcct cggcgttggc
     1201 ggatttcagt tcctcatcgg tgagcgtgac atatcgcccc tcttcgtatt cataaccctt
     1261 gaccacgcgt gagcgtggga tcgactcacc ggtggacttg ttgattaggt tgtagccgac
     1321 cggcgagaga tcctcgctgt cgagcatgcg gaggtcgagg tcctcggaac tctccgcggg
     1381 atagaggccg atggggatcg tcaccaggcc gaattccagc gttcccttcc agactgcgtg
     1441 cgccatcgtg gttgcctcgt gagcggcggc ccggaaagtt gtccgggtgc cgggaatttt
     1501 ctgccctggt gaaaaagtgg cccgggtcgg tcggcgcacc gcactcctga caaccgttcc
     1561 acttcggagt gcgacaggat ccggtaaacg gtaaaaacgg tccctgtgca tccgctgtcg
     1621 ccgggccgac tacaaagagg ttgcaaggca tatgccacgg agtggcgttg cccggatggc
     1681 ccgttttgac acggtcggaa cgcttcttgc gttgatacca ccgcatgaat cgcaaagcgt
     1741 gtgatgccgc gggtaaccga tcgctctggg cgttgccgat gccggaaatc acggtgaccg
     1801 gccgcaacct ggacgaggac ctcaccacgg atgtgtgcgt ggtgggaggc ggcatcgccg
     1861 gactcacgtc agcgtacctg gcgctcctcg atggggcctc ggtcgtcctc atcgactcgt
     1921 gggacatcgc ggggggcgaa agctgccgca ccagcgcgca cctctccaac atgcttgacc
     1981 ggcgttacgt ggcgatcgag tcgatccacg gcgaggatgg ggcgttcctc gccgcggcca
     2041 gtcactcggc ggccatcgac cggatcgaac ggatcgcgcg cgacgagtcg atcgactgcc
     2101 gcttcgcgag gctcgacggc tatctctttt cgccgccggg aggttcgctc gacgatctcg
     2161 agaaagagct gaccgccacc cggcgggccg gcctggcggt ggacctgatt gcccgcgcac
     2221 cccttccgga cttcgacacg ggaccgtgcc tgcgcgtggg aggccaggcg cacttcgacc
     2281 cgacgcgcta tctggcggga ctggccgccg ccatcgtccg tcgcggtggc aggctgtacg
     2341 ccgattcccc ggccgaaagc gtggccggcg gggccgagcc ggtcgtgacg atggccaaca
     2401 ggcggaccat ccgggcgaaa gccgtgatcg tggccaccaa ctcgccgatg aacgattacg
     2461 tatcgatcca cctgatgcag aaggcgtttc gcacctacgt ggtgggtggc ctcgtgccgg
     2521 gcgacaccat tgcccgcgcg cttttctggg acaccgccga tccgttccac tatgtgaacg
     2581 tgatcccggc cgcggcgctg gtcggccgtt cggtgcccga cgggcacgac ctgctcctca
     2641 tcggcggcga agatcatcgg accggtcgtg gcgacccgac ggcggaacga ttcgaccagc
     2701 tcatcgagtg ggccgcggcg cgctttcccg gcatcgggcc gttcgtcgtg cgatggtcgg
     2761 ggcaggtgct cgaatcgatc gatggacttg gcctcatcgg ggcggatccc gagaagcggg
     2821 ccaatgtgta catcgccacc ggcgattcgg gaaacggcct cacgcacggg acgatcgccg
     2881 gaatgctcct cacggacctg atcgccggtc gttcaaaccc ctggcgcgac ctctattccc
     2941 cgcgccgcaa accggtgggc gcgctcggca atttcgtgga agagaacgcg cggatgacgt
     3001 accagtacgc cgactggctg cacgggagcg aagtgcacga cgaatccagc atcgctcccg
     3061 gcagcggcgc cgtgctgcgc gaagggctca agaagtggac ggccgtgcac cgccgcatgg
     3121 acggcacggt agtacgccgc tcggccgtat gtccccacct cgggtgcatc gtccactgga
     3181 atgccgccga atgctcgtgg gactgcccct gccacggttc gcgattcgat gccgagggga
     3241 gcctgctcaa tgggcccgca ttgacgggac tggcggaggc gccggatgcg cgggaggggg
     3301 ccggcgcgga cgagacggcg aagccggctt cgggatagcg ctacgcgttc accacttccc
     3361 caccgttcgg atgcagcacc tgccccgtga tgtacgaggc gtcgtccgag gccaggaaca
     3421 cgtagctcgg cgccacttct tccggttggc cggcccgccc catcggcacg tcactcccga
     3481 acgtggccac atccttcgga cggaacgtgg cgggaatgag cggcgtccag atcgggcccg
     3541 gcgccacggc gttcacgcgg ataccgcgct cgacgagcgc catggccagg gatcgcgtga
     3601 gccccacgag cgccgccttg gtggcggcgt aatcaacgag gtgcggactg ccgcgatagg
     3661 cggtgaccga cgtcgtgttg atgatgcacc cccccttcgg caggtgaggg agagcgtgcc
     3721 ggatgaagtg aaaagcgccg aagacattgg tgcggaatgt ggcttccacc tgtcgggccg
     3781 tcatcgcctc cggcgactcc acactgtgct gctccgccgc gttgttcacg aggatgtcga
     3841 gcgcaccgag ttccttgacg gttcgtgcca ccgcctgacg acagaatggc tccttgccga
     3901 tgtcgcccgg aaggaggagg acgcgcgccc cctcccgttc caccaggcgg gcggtttctt
     3961 tcgcgtcggc gtgctcgtcc ttgtagacga tggccacccg cgccccttcg cgcgcgaaga
     4021 ggatggccac ggcgcgcccg atgccgctgt cgcctcccgt gatgagcgcc accttgccgg
     4081 ccagcttgcc ggtgccacga tagcggtcat cgaccgatac gggctgcggg cgcatcgggg
     4141 cctgcttccc cggctggcgg gactggcgct gtgccgggcg ggccacacgt ttcttcctgg
     4201 tcttggcggg catggaaacg aaacgctgca atccccgtgc ccgtgcgagg ggatgaaatt
     4261 ggggaccgcg ccacgcccga gagcgtgaac acggcccccc tgaaccggtc gtcgatggat
     4321 gtgatgcgcg ttagagggac cgcctcagta cgccgatggt cggagtgttc cgactacttc
     4381 tcgtcttcgg tgtcgtcggc gcccgagagc tgtgcgcgga tggcgcccga cgggtatgtc
     4441 tcgttgtgca cgttcacgta gaacatgtcg gggttctgga ggatctcgcg cgcgaggtcc
     4501 ttgtcgatcg tgatgcagtc gttggagctg cctgtatccg agacttccag cggcacgacc
     4561 acggggcccg cctcgccgat cggtgcgcga tggatgtggg ccgcggtggc gccatcgagg
     4621 ccgtcccagg tgagcgtgta gcagatttcg ccgaggccgg gattgacggt gagcttcagg
     4681 gcgccggtgc cgtcgggatc gcccggaccg ggaacttcgc tggcgccgct gagcgtgacg
     4741 ttgaacggct ttccgccgcc ctgggacatg ccggcgaaca gtgcgacgag cgccagggac
     4801 gcgacggcgc cccagatcat cttcttcatg atgcgaactc ctcgttggat gggggtcggg
     4861 cgatccatcg cggccgcgcc cggggttctt cgtggcgtta ccacccggct ggaggcaagg
     4921 agcgggccat ccgaaatcgg ttcgaaatga cacgattcga gtcgatgtgc ggctctgttc
     4981 cgaggtggcc ctgccggatt ctcccggggg gtcgcgcatc gtttcccggg gcggggagat
     5041 tcgttcccct ctgggggaaa gcccggtcga gagaccgcgg gcctgattgc cgtccacccc
     5101 cgaacgccca tcgtccggat ccggacgatg ggacggggcg ccatcgccgt aagtcgttgc
     5161 catttcgcgt ttccgtgctg gcactccggt tgcagttgcc ggggcgttca cggaggaaac
     5221 catggatcga ccgatcgaca gcgcggtgat ggaaggacgg aaacggcggc ggtggatcac
     5281 gctggccggc gcggcggccg tcctcgtgct cctgatggcg ctccttcccg catgggtaag
     5341 gccggcggtg gaacgcgggc ggctccggac cggaagagtg gatcgcggcc cgatcgagat
     5401 gacgctctcg gcggccggca ccgtcgtgcc ggaagtggag cgcacgatcg tggcacccgg
     5461 cccgagccgc gtgctgcggc tcctcaagca gccgggcgat tcggtggcgg tgggcgagcc
     5521 gatcctcgag ctcgacaacg gcgcctcgcg cctgacggtg gagcgcctca cccggcagat
     5581 ggcgatgaag gagaacgagc gcgagcaggc gcgcgtggac caggacaatc gcctctccga
     5641 gctgcgcacg cagagcgaca tcaagagcct ggaactcaag tcgaagcagt tcgacgtgga
     5701 gcgcaacacg aagctcaagg agatgggcgt gatctccggc gacgtgctcc gccagagcca
     5761 gaacgacacc gaacgactgg ccatcgagat cgccgagctg acgcgccgcg cggccaacgc
     5821 cgacagggaa ctggccgtcc ggctcaagtc gctcgacctc gagtacgaca ttctcgcgaa
     5881 agaacgcgac gaggcggccc gcgtgctgga ccgcggcacg gcctcgagcg acatcgccgg
     5941 cgtgctcacc tgggtgctcg gcaccgaggg aaccgccgtg aacgccggcg atgaactggc
     6001 ccgggtggcc gacctcgatc gcttcaaggt ggatgccacg ctctccgacg tctatgccgc
     6061 gcgcatccgc cccgggatgc cggccttcgt ggccgccggg gaggcgcgac ttcccggtcg
     6121 tgtgtcgcgc gtgctgccca cggtggacgt gggaacgctc cggctcgaag tggcgctcga
     6181 cgatcagtcg aacgctgtcc tgcgccacaa cctccgcgtg gaagcccacg tgatcgtcga
     6241 ccggcgcgag ggagccctgc gcgtggcgcg tggcacgttt cccacgatcg acggcaacct
     6301 ctgcgccttc gtcgtccgcg gcgaccgcgc ggtgcgccgc cccatccgcc tcggcctgac
     6361 cggctgggaa gcggccgaaa tcctggaagg actgaacgag ggcgacgaag tcgtcctctc
     6421 cgacatgagc gaatacctga accagaagga gatacgtatc cgatgaacgc catcgccacc
     6481 ctcgaacgcg ccgcggacgc gatcgccggc atccggcgtc accccgccat ccgcctggtc
     6541 gacgtcaaca agatctatcg caccgaccgc atcgaaaccg tggcgctcga cggcgtgaac
     6601 ctcaccgtgg agcccggcga gttcatcagc atcatgggcc cgtcgggctc ggggaagagc
     6661 acgctgctcc acctggtggg gctgctcgac cgcccctcga ccggcacgct ggagatcacc
     6721 ggacggccgg tgatggggct gaacgatcgc gcgctcgccc ggctccgcaa cgagacgatc
     6781 ggcttcgtgt tccagacgtt ccacctcgtc aatgacctga acgtggtgga caacgtggag
     6841 atccccctgc tttaccggaa gatgtcgggt tcggaccggc ggcgccgggc catggaggcg
     6901 ctggcgcgcg tggggctgtc ggcccgcacg caccatcacc cgggacagct ctccggcggc
     6961 cagcgtcagc gcgtggccat cgcccgggcc atcgccgccg agccgcggat cctcctggcc
     7021 gacgaaccga ccggcaatct cgacagccag atgggtgacg agatcatggc catcctcgag
     7081 agcctgaacc gcgagcaggg aacgaccgtg gtcatggtca cgcatgacca gcgcctggcc
     7141 gagcggacgc gtcgcaccat ccggctgttc gacggccggc aggtgcagta ggagggacga
     7201 tgttcctgag ccacctgaaa atcgcgctga aagtgctgtt gcgccgcaag ttcttcacgt
     7261 tcgtcagcct gttcggcacg gccttcacgc tggtcgtgct gatggtgatc gcggccatga
     7321 tggacggttt cctttcggcc ggcggccccg agcccaacct cgatcgcacg ctctacatct
     7381 cgcggatggc catgcgcggt cccgaaaacg agtggaacgg caacccgggc tacggcttcc
     7441 tcgacaagta ctgccgcgac cttcccggcg tgcaggtgat gtcgctctac accgatgccg
     7501 gaaatacgat cagcttcctc gacggcaaca agatcacctc ggacctccgg cgcacggacg
     7561 cgcaatactg gaccattcac cgataccgct tcctggaggg ggggccgttc accgaggacg
     7621 acgtgaccag cgcgcggccg gtggcggtca tcaacgccac gacgcgtcat cgcttcttcg
     7681 gagacggcac cgcggtcggg cgcacgctgg aaaccggcgg gcgcagctac accgtggtgg
     7741 gcgtggtggc cgacgtatcg cgggcgcggg acgcggtgta cggcgacatc tgggcgccgc
     7801 tcaccaccgc gaaaaccgac gattacagga cgcgactgat gggcagcttc agcgccgcac
     7861 tcctgacgcg ctcccgcgcc gatttcccgg cgatccgcgc cgagttcgcc cgccgtctcc
     7921 ccaccgtcca gatgtcggaa ccggagcggt acgaccgcat ggaaggcgcg ccgcgcacgc
     7981 gcttcgaaga catggcgggc gagatcgccg gcacggaagg gccttccgag aaggcccgca
     8041 cgacgctctt cctgttcggc gccacgctgg ccgcgctcct cttcatgtca ctgccggcca
     8101 tcaacctcgt caacctgaac atcagccgca tcctcgagcg ctcgagcgag atcggcatcc
     8161 gcaaggcgtt cggcgccacg tcgggccacc tggtcggcca gttcctgctc gagaacatcg
     8221 tgctggtgct gatgggcggc ctgatcgggc tcgcggcctc ggcatggatc ctcgacctca
     8281 tcaaccg
//
LOCUS       DASVOD010000203         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_325792, whole genome shotgun sequence.
ACCESSION   DASVOD010000203 DASVOD010000000
VERSION     DASVOD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325792"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /gene="ligA"
                     /locus_tag="VF720_04290"
     CDS             complement(<1..1723)
                     /gene="ligA"
                     /locus_tag="VF720_04290"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX7878600.1"
                     /translation="MAKKALPKEPALRIEALRAEIRRHDRLYHVEATPEISDRDYDRL
                     VEELLDLEAGHPELVTPDSPSQRVGGEPLTGFVTVRHAVPMQSLDNTYNEGELKAFDE
                     RVRKVLGDPVEPVRYVVEPKIDGVAVNLVWEDGLFTLGTTRGDGVSGDDITQNLKTVR
                     GLPLRLNDVALPIGRTTAGRFEARGECYLTREGFSAMNARFEAEGEKTFMNPRNATAG
                     TLKQLDPSIPGSRPLKILTYQVVEAERRHGLRTQSSILAALRDAGLPVNTSDTVHGID
                     EVIRLVSVWDAKRHKLPFDVDGLVVKVDDLRQQEDLGSTAKSPRWAIAFKFPGEEAIS
                     QVEQIFPSVGRTGVVTPIAFLTPVLVSGTMVSRASLHNADEVARLDVREGDWVAVEKG
                     GEIIPKVTRVLTERRTGELKEFRMPATCPSCGSDLVREEGEVAWRCIRVDCPAQVEGR
                     ITHYASRGAMKIEGLGEKVVAALIAAGLATNAADLYDLTAEQLVPLERMGEKSAANLV
                     ASIEASKTRGLSRLIYAIGIRQVGTRAAQLLARHFGLMAALAAADDEALVAVEEIGPK
                     VAHEIRRF"
     gene            complement(1723..>2127)
                     /locus_tag="VF720_04295"
     CDS             complement(1723..>2127)
                     /locus_tag="VF720_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7878601.1"
                     /translation="LPGMIARGAGHVVHIGSIAGHEVYPNGNVYCATKSAVDALTRAL
                     RLDLMGTGVKVSTVDPGLVETEFSLVRFKGDVERARRPYVDLEPLTATDVAESVLFVV
                     SRPAHVTVAEVILVARNQASTRDYFRKPRETA"
BASE COUNT          317 a          742 c          701 g          367 t
ORIGIN      
        1 agaagcggcg gatctcgtgt gccaccttcg ggccgatttc ctcgacggcc accagtgcct
       61 cgtcatccgc ggcggccagc gcggccatca atccgaagtg gcgggccagg agctgggcgg
      121 cgcgcgtgcc gacctggcgg atgccgatgg catagatgag gcgtgagagt ccccgcgtct
      181 tcgaggcctc gatcgacgcc accaggttgg ccgccgattt ttcgcccatc cgttcgagcg
      241 ggacgagctg ttcggccgtc aggtcgtaca ggtcggccgc gttggtcgcg agccccgcgg
      301 cgatcagggc cgccacgact ttctcgccca gtccctcgat cttcatcgcg ccacggctgg
      361 cgtagtgggt gatgcgtcct tccacctgcg ccgggcaatc cacccggatg caacgccagg
      421 ccacctctcc ctcttcgcgc acgaggtcac tgccgcacga cgggcaggtg gccggcatcc
      481 ggaattcctt gagctcgccg gttcgccgct cggtcaagac gcgcgtgacc ttggggatga
      541 tctccccccc cttttccacc gccacccagt ccccctcacg gacatcgagg cgggccacct
      601 cgtccgcatt gtggagtgac gcgcgcgaca ccatcgtgcc ggacaccagg accggcgtga
      661 ggaacgcgat cggcgtgacc acgccggtcc gtccgacgga agggaagatc tgctccacct
      721 ggctgatcgc ctcttcgccc ggaaacttga aggcgatggc ccagcgcggc gacttggcgg
      781 tggagcccag gtcttcctgc tggcggaggt cgtcgacctt cacgacgagg ccgtcaacat
      841 cgaacgggag cttgtgccgt ttcgcgtccc acacgctcac caggcggatg acctcgtcga
      901 tcccgtgcac ggtgtcgctc gtgttgaccg gcaggccggc gtcgcgaagc gccgccagga
      961 tggacgactg cgtgcgaagt ccatgacgcc gctcggcctc cacgacctgg tacgtgagga
     1021 tcttgagggg tcgcgagccg gggatcgagg gatcgagctg cttcaacgta ccggcggtgg
     1081 cgttccgggg attcatgaac gtcttctctc cttcggcctc gaaccgcgcg ttcatggcac
     1141 tgaacccctc gcgcgtgagg tagcactcgc cccgagcctc gaagcgcccc gccgtggtcc
     1201 gcccgatcgg gagcgccacg tcattcaacc gcagcggaag gccgcgcacg gttttcaggt
     1261 tctgggtgat gtcatcgccc gagacgccgt ctccccgggt cgtgccgagc gtgaaaaggc
     1321 cgtcttccca gaccaggttg accgccacgc cgtcgatctt gggctccacg acataccgaa
     1381 ccggttcgac cggatcgccc agcaccttgc gcacccgctc gtcgaacgcc ttgagctcgc
     1441 cctcgttgta ggtgttgtcg aggctctgca tcggcacggc gtggcgcacc gtcacgaacc
     1501 ccgtgagcgg ctcccctccg accctctggc tcgggctgtc cggcgtgacc agctcgggat
     1561 ggccggcctc gaggtcgagc agctcctcga caagccggtc gtagtcgcga tcggagattt
     1621 cgggggtggc ctcgacgtgg tacaggcgat cgtggcgacg gatttccgcg cgcagcgcct
     1681 cgatccggag cgcgggctcc ttcggcaagg ccttcttcgc catcaggccg tctcgcgcgg
     1741 cttccggaag tagtcgcgcg ttgaggcctg gttgcgggcc accaggatca cctcggccac
     1801 cgtcacatgg gccgggcggc tcacgacgaa gaggaccgac tcggccacgt cggtggcggt
     1861 cagcggctcc agatcaacgt acgggcgcct ggcccgttcc acgtctccct tgaagcggac
     1921 gaggctgaat tcggtttcga cgaggccggg atcgaccgtg gagaccttga ccccggtccc
     1981 catcaggtcg agtcggagcg cgcgcgtgag cgcgtcgacc gccgatttcg tggcgcagta
     2041 cacgttgccg ttgggataca cctcgtgccc cgcgatcgaa ccgatgtgca ccacgtgccc
     2101 cgcaccgcgg gcgatcatcc cggggag
//
LOCUS       DASVOD010000204        18866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_639063, whole genome shotgun sequence.
ACCESSION   DASVOD010000204 DASVOD010000000
VERSION     DASVOD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18866
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639063"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..737
                     /locus_tag="VF720_04300"
     CDS             132..737
                     /locus_tag="VF720_04300"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEX7878602.1"
                     /translation="MSLVHHEPRDIGWIETICGSMFSGKTEELIRRLRRAAIARQRVQ
                     VFKPVIDDRYDTEKIASHTGQTFESRPILEIEQVDELLHRDTRVVGLDEAQFFDEKLV
                     DVCESLADRGLRVIVATLDQDFRGEPFPAIPRLMCASEYVTKMQAICVVCGNPASRSQ
                     RLSGGSSQVQVGGKDFYEARCRGCWRPYVIAQGELPEVGGE"
     gene            757..1710
                     /locus_tag="VF720_04305"
     CDS             757..1710
                     /locus_tag="VF720_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878603.1"
                     /translation="MRQSLLMIAALAASMVMAVEAHAQFPQTDIYYIRLVTFGNTGAR
                     ADTVINVTRRAGYDNQPSVSPAYPNNCWYSSIREDGQADVYKLDLSRWPTVSSERVTS
                     TSEAEYSPRVMQDGEHLTVVRVEADSSQRLVQMDPDGANPTRLLSNIPDLGLIGYYAG
                     LDDKRLLLFILGEPHALWMVDVTTGTRKKLADDIGRCFRPMPWVGPDAIAYVQKDSTG
                     SQLCELDVTTGKRRTLMPMPDRTVEDFEWVDVSARGPTRLVLTAFDGRILFATLGESD
                     AWKTWIDLTPYRLTALARLAWRPGADFGLFVVAADTLAAPK"
     gene            1897..4974
                     /locus_tag="VF720_04310"
     CDS             1897..4974
                     /locus_tag="VF720_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HEX7878604.1"
                     /translation="MPRSERSRTPLRALGLGVGRCLVFLLMVIAGSPAIARAQGPNDP
                     PDSAVVRVAVRTDKQVYEPGEPIRITVTATNPLDRAITLTFNSTLQADYGFDNAWQWS
                     DHRGFGDAITHVTIKANETWTFPSFTHLPADYFPPPGRHAVRGIVTGYGEAQVTIGVG
                     GNTPPPSPLHLTPEIVPNPVPFGVPIEMFVTVTNLSSTTQTFGHSGCPVHFTVDQHYS
                     PPVACAEYWRQVTLEAGESIRFGPPEFDYLTLGRAESGFVLTPGVHVVDFEVPGVGKT
                     AARIQVAGGGGSISGSVVGLDGQLLHHAVVQAVTYATPDSTPWNGGNFVHQTFTDEHG
                     QYRFDGLPSDLYFVNASYNSSPPVWYPGVPTRDMATPLPVSEGTNHQNIDFALNQTGP
                     PPPPTKVVTGTVLGNPPPQSERCCPAPLAGAVVAAVAERASAVDDTILPPPPPDGGGS
                     GGPPVPGDPTRPGEPWPGQPPVFYAFADSVGDFSLPLPAGRYRFVAFNQGFRYQWWEA
                     TTDWSESDVIEINPYTRSLGPDLMFTLDPLPGPNGGAGAVITGTVHGLIQADDPATGD
                     PRGLPPIQMVPIEGADVVARPLVLDPRILWFVEVHYPVRTDADGRYRIELPADLPYVV
                     QTWAAGWESQFYDHAWSRIEARPVDVAPGAETTSIDFDLHQGYKPPDLGVIAGSVFRA
                     GTDDVGSNNDPARIYPVGGATVRVRLAGPGFGGFDRVAMTAEDGTFRLDGLPMADDGS
                     LKYLVSAEAEGSLPAYFPDAMSWQEATPLAPGNDGQRSPVRIVLTPRQAEGAFFLVGV
                     VRGDPGSGVPPVELPPWPGDDIADAVADSSGIPWWPLIGAYLYLVPADDPSQGPVAGG
                     ATSDNGTLIIPHLAPGRYKAYADHPGFHTGWFHGANRAEAAVLTVTDGEPLLVDLVLE
                     TAFPPPGSGGDGNDAAAPAMVSGLRNSPNPFKPQTTIQYRLLAPADVSVQVYDLNGRL
                     VRTLISAVHQPAGEQFLPWDGKDETGVEASGGVYFYRVWTPVETRTGKMVMVR"
     gene            4971..5360
                     /locus_tag="VF720_04315"
     CDS             4971..5360
                     /locus_tag="VF720_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878605.1"
                     /translation="MSVSHAIRALTTALMIGLGAAGLSGCADDGNKEPIETLAPDMVR
                     LTGSVSEIDDDVPADGGVTIRMTDKVKGAVTLNFESLFTLPRPTEARQALYADIQKVS
                     AGDVVTATARLVEGNYRLESIRVIEDH"
     gene            complement(5390..6601)
                     /locus_tag="VF720_04320"
     CDS             complement(5390..6601)
                     /locus_tag="VF720_04320"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX7878606.1"
                     /translation="MSPKPLEGILVLDLSRVLAGPWCTMVLGDMGARVIKVESLSGDD
                     TRAYGPPFAAGESLYFLSINRNKESVTLDFQKPEGAALLRKLVARADVLIENFRPGTL
                     ERHGFGYADCAALNPRLVYASITGYGQEGPWRDRPGYDLVTQGEGGVMYLTGQADGPP
                     LKCGVSQADITTGMWMVGGITTALYVRERSGTGQQIDLALADSQLSLLGFAGADWLVA
                     GHEPARMGNLHPMLAPYATMAASDGWFNVAVGTPGQWRAFCTAVERPELADDARFATN
                     ADRVRHREALDDILLPLFRARPRAYWLARLAAADVPSGAVRKVSEALESEQAAARNMV
                     VTLPHPTIGPIRQVGLPLNFSATPGAPRSAPPRLGEHTDAVLAELCGTTADELADLRS
                     RGIVGAPPTGS"
     gene            complement(6598..7017)
                     /locus_tag="VF720_04325"
     CDS             complement(6598..7017)
                     /locus_tag="VF720_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX7878607.1"
                     /translation="MKTFRREIWMNIPKRRAFVNITPEVESALAESGIREGLVLVNAM
                     HISASVFINDDESGLHHDYEEWLEKLAPHAPVDQYRHNDTGEDNADAHLKRQVMGREV
                     VVAVTAGKLDFGPWEQIFYGEFDGRRKKRALVKIIGE"
     gene            complement(7083..8477)
                     /locus_tag="VF720_04330"
     CDS             complement(7083..8477)
                     /locus_tag="VF720_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX7878608.1"
                     /translation="MRVILPASPPLALMLLAILAAPTPGLAGQGTRFEDFQGPDDHNS
                     VTTMRTWTIELRPGTDWLEVTLPRPEALSPGGDVGVFNQETTTWYLSSPPHVEEDRSE
                     WYTRLTWPPGALNGGARVEIREETDSSSDVTRGGLHAEPVRRPGDWYTSPSLTIPWVA
                     GIDEAVRWAAETSQWDDGLPFGEHGVVQRWMAYVNEAIDVRLVISIWAPENAASAWNA
                     GLSDPWGQADVFASGLRALGFPTCVGTLMRPDGEYVNLADRAFNLMGVAPWVGVWCDG
                     LGFLPVDLRARHYGFLASNVQVGNYVEDFQHLRWIVDSNDPTARVEFDDSRHEYERPP
                     STRQRVRTRRIDDGGIDTFICQDTPGHHAPGPVTAVPDTPASRGELLIASHPFRQVLS
                     LALRLDRGGEVTVTVFDVAGRRVALLADARAVAAGETFFRWLPDTSPAGVYFVQARLD
                     DGRSFTARAVRAGS"
     gene            complement(8474..9091)
                     /locus_tag="VF720_04335"
     CDS             complement(8474..9091)
                     /locus_tag="VF720_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878609.1"
                     /translation="MMRRLELPGRAPGPYRRGGPLLAACVAAVLTVGMSSVRLPAVEP
                     ARDGVTLRVDRAWRAEFFPGAGRDGVRRSSIWRVTATLGRGLSTSAPWYLVVGPDAGA
                     PDVVDAAAIEGAVAEALPRDSGHPTDSDIIPRHPGGRRHPIGAPDWRDAAGWNAWRLH
                     ASSPLRRTLALSLDAATVDTLGRWVGLFDASGHRVAIARLDEAGR"
     gene            complement(9088..9366)
                     /locus_tag="VF720_04340"
     CDS             complement(9088..9366)
                     /locus_tag="VF720_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878610.1"
                     /translation="MFFRFFLEVMTALVVGSYTGPAGQAPACHAPQVTTRQVDAPEVA
                     GPRLAAPMRIRPGAVRSGGGPRPPKKPPEKRPHDSSWSEIKSRFGGRP"
     gene            9604..10467
                     /locus_tag="VF720_04345"
     CDS             9604..10467
                     /locus_tag="VF720_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7878611.1"
                     /translation="MPGPIVYRPPVRPQQQPDWAPPPFGPPIVIRPNWPLHIALFLAT
                     IATTWFTGGPLYAASVLAILLSHEMGHYLTALHYRVPATPPYFIPMPFGLFGTFGAVI
                     RMATQGANRRILFDIAVAGPIAGMVLAVPACVIGLRLSRVVTVANAGEIHLGSSLLFQ
                     WLSDLVVGKLGPGQDIELHPVAFAGWAGLFVTSLNLIPVGQLDGGHVAYALLGRRAHK
                     LAYLVAAGFVALTIFVSWSWLVVAVMVALFGLRHPPTGDDWTPVDTKRMILGVFLLLM
                     FVITFTPRPLQ"
     gene            10480..11607
                     /locus_tag="VF720_04350"
     CDS             10480..11607
                     /locus_tag="VF720_04350"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7878612.1"
                     /translation="MPELPEIATMVKALKSRIIGRVVTHSVFEESGLLDGGSPEQARK
                     AILGREIVDVARCGKYVRIDFAGTIGGDILDLIEPRRKGGARKMKPKAAPLPPDASLV
                     VRRPATLADALVLADGDDWIHGAAIDALRTTEKRGHGAPLTAPARQRLLAARRRQFGR
                     YASGERSSLLVHLKMTGRWYLMNDNGQMLPPRTRFAIMLTFEDENLVLGMKDVRRLGT
                     ARVLTGAAAHAWPADLELGPDALTTRWTGNTLKKRVSGSLPIKLALLDQSRLAGVGNI
                     YASEALHRAAIHPARPADSLTNEEWQRLAVTIPDLLRHSIRNWCHLSRWVGPSVEGYG
                     NFNGELGVYDRRGESCRRCKGIIHSMVQGARTTFYCPGCQA"
     gene            complement(11611..14148)
                     /locus_tag="VF720_04355"
     CDS             complement(11611..14148)
                     /locus_tag="VF720_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7878613.1"
                     /translation="MPSAVRKVGFICLPDRQLDIFSEITRRGEFRPEVVVDSNPASCA
                     FKMAEILEIPASGDLELLRRYPCDLLVIPDDRPDLQVDVCRVLGSALAGIMTGTEMAT
                     LLDLRAEGSATEEPSSTWMLPLPAAAAAAAAETATATAPQASSPAQVHRDMEDDSPLQ
                     IERHPLERVDAVFENVDAPEVQEEAPRSGHRDYELEVIADPAWFDTAAHASGDLKRIS
                     ETLDLAGDQQRLLKRILEIAVNTAGGDSGSIMLLDESGQHLRIVVADGLSDEVIRKTS
                     QRRGEGVAGQVLRDGRARVLVDKLDDPRYRSGRERSGIQSAISAPIQVAGRTIGVLNV
                     SSDSRPNAFTAHSLEQMERFGSEVAEVLLRALKPAGGERPLARSFEIAVESLMTLDRP
                     LDHRLEAVLHKLATGTRAAAGRLYLLDGTGRRLDVAAEFGSAAPGSRRSTVMADRGFT
                     ARALDRNESTVLTSTIDATGPRALLLLPLRGAEPMGLIELEDLPAEEPGWSERLEALT
                     QAAARLTTRLEFERSQVLLTRRTRQMLRLSDLSGGLLSATSAGELNRLAVEAAAEIVD
                     ADLAILRRPDSDNLELSDADFAIGDPEGQRLLLLDAEFGELAALGGRVVSLDECPTLL
                     EERLGNEAGVRWALALPIPGIPGGVLSVYRRKGTALAGDSEDRALLVRLSASLGQTLV
                     RMEAGELPGGDTDRFMRWTVFQERIAEEMKRADRYNRTFALVTLELEGIRAMAESAGG
                     DWLEGARFALTDFVLNQIRELDIPCWVREGRVAIICPDTSELGDSLADRLSEEWRKLL
                     PSLPLAGLDRMAFNVNETIYPLDRRDWESMLDWAADRFDTGDTRQAS"
     gene            14330..15808
                     /locus_tag="VF720_04360"
     CDS             14330..15808
                     /locus_tag="VF720_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IG family 5'-nucleotidase"
                     /protein_id="HEX7878614.1"
                     /translation="MSELPTTTENRVAVVEERRISKQGRVFVNRNLRLPSLGAIGFDL
                     DHTLAHYAPIPVETLAFDVTKRKLVENKGYPAAILDYHYDPRFVTRGLVIDKKRGNII
                     KMNYHGYVSRAYHGRKELHTEDRKKQYRQRPIHLSNTGGYASVDTLFHLPEVYLYLLL
                     VEMNQAAPRKDRRSFSQIYTDVREMIDEAHRDGTIKTVIVADPGKYLAPDPRMGPTLE
                     HFRSAGKKLFLLTNSEYAYTDVLLSFLLKHNLAGKQSWRDFFDIIIVDARKPDFFTHK
                     ENDEPRPHHEELPGPPIWSGGGARFLEDRLGYQGDRILYFGDHTYGDILRSKKSVGWR
                     TAMVIEEMEREIEALEMIRPKMIRLGTLAALRERLDADMAFVRRERNKLALIAENLGG
                     SNPAVVAKVQGRLTRREDELARMQSELSSAIKESRQLRKENNEAFNSFWGSIFREGNE
                     TSRFGHQVEDFACLYTARVSNFLNYPASQYFQAPIRLMPHEI"
     gene            16002..16715
                     /locus_tag="VF720_04365"
     CDS             16002..16715
                     /locus_tag="VF720_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878615.1"
                     /translation="MRTRYLAVLFGIAALALLPSIAPANPIPGLYNSTDLGGQLLTGR
                     ASTWRPGINSGLPHVLHAQSWDSATLGTQWDVSCAIETTPFSVQDNRIAGTGTVVYTS
                     TFQGGTLTLYPGAWPWGDGTATLGTTTLISTVQFVNNIPVASVVNGNTSGEFTNGCNL
                     VFAIGNGSGVGETTSLNPLITKPATFPGFLDGTCGPAGPVVQFGTWGTTLTQTMAIDC
                     GALPVEPSTWSAVKGLRLD"
     gene            16869..18248
                     /locus_tag="VF720_04370"
     CDS             16869..18248
                     /locus_tag="VF720_04370"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX7878616.1"
                     /translation="MLLAALTGFLLTGTVNQFGQSYRFELAAASGGAFDFRKEGPAGE
                     RLVWDGSSGWRVDADGRVMPLELFDLEQTRLTAWLAVGSTLGWRANVSADTLVLPGGR
                     LVTRVTRNSDGDIALATWPGVSGEVAWSFDWTTAADGVPDVAARWTQRVGDLVVETMS
                     VTDVNRNAEFDPSRFAEPPRPADDAIWLPEAPDTLEIMRAQTGHLLIRPRLNGRDVGW
                     FIFDTGAGESVVTPAVAAQLGLPEVGRTVLTSILGSAESPVYGVDTLDVGRLRLAHRR
                     LTAMDLAPFEGPLGVPLSGVLGGEVLSRAILTVETAVPRLTVQKPDEVTVDAAAWRPL
                     HFNQRLPLITADFAGGTGELFRIDVGAGGSFGHVAFHSPAVARLGLLAGNDSERLEAA
                     GMTCVFAPIAWFNLAGHRFENEPVVYVEKSEGPMADSWVTGNIGVELLKPFRVIFDCP
                     NRRIAFVPR"
     gene            18272..18835
                     /locus_tag="VF720_04375"
     CDS             18272..18835
                     /locus_tag="VF720_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7878617.1"
                     /translation="MGRRITTALLSRPAQAKALSQPIRLEIVEAFRPGEELGVDELAA
                     RLGRKPVSLYFHVRNLVANDILVAADERRGAGRPTRLYRVAADRLMVSPSPKASRDIQ
                     AGFDLFRATLRATERDFRKALALYARPGSVQPTRMAGQRMKGRLSDRELKIVEGLLTR
                     LENIFINATGRRSGRLVAVTTVMVPIG"
BASE COUNT         3039 a         6478 c         6287 g         3062 t
ORIGIN      
        1 atcaggggcc gccgtgggac agcaggcccc taccgtctga ttctgaaaca gttcctttcc
       61 gatttgcatg gggcgatgcc ccatgcaagg tcgtcatgcg ataatcggtg ccaggaccgc
      121 ccgccaggag aatgtctctc gtgcaccatg aaccccgcga catcggctgg atcgaaacca
      181 tctgcggcag catgttttcc ggcaagaccg aggaactcat acgccgcctc cgccgcgccg
      241 ccatcgcgcg ccagcgcgta caggtgttca agcccgtcat cgatgaccgc tacgacaccg
      301 aaaagatcgc cagccacacc ggccagacgt tcgaatcccg ccccatcctt gaaatcgagc
      361 aggtggacga gctgctccac cgcgacacgc gcgtggtggg cctggacgaa gcacaattct
      421 tcgacgagaa actcgtggac gtttgcgaat ccctggccga ccgcggtctg cgggtcatcg
      481 tggccacgct cgaccaggac ttccgcggcg agccctttcc ggccattccg cgcctcatgt
      541 gcgccagcga gtacgtcacg aagatgcagg ccatctgcgt ggtgtgcggc aaccccgcca
      601 gccgcagcca gcgcctctcc ggcggttcca gccaggtgca ggtcggcggc aaggacttct
      661 acgaggcgcg ctgccgtggc tgctggcgcc cgtacgtgat cgcgcagggg gagttgccgg
      721 aggtgggtgg ggaatgactg gccgcgagga atgcggatga ggcagtcact cctgatgatc
      781 gcggcgctgg cggcatcgat ggtgatggcc gtcgaggcgc acgcccagtt cccgcaaacc
      841 gacatctact acatccggct tgtcactttt ggcaacacgg gtgcccgggc cgatacggtg
      901 atcaacgtga cgcgccgggc cggctacgat aaccagccgt cggtgtcgcc tgcctatccg
      961 aacaactgct ggtacagctc gatccgcgag gatggccagg cggacgtgta caagctcgac
     1021 ctgtcgcggt ggcccaccgt gtcgagcgag cgcgtcacct caacgagcga ggcggaatat
     1081 tcgccgcgcg tcatgcagga cggtgagcat ctcacggttg tccgcgtcga ggccgactcg
     1141 agccagcgcc tggtacagat ggatccggac ggcgcaaacc ccacccgtct gttgtcgaac
     1201 atccccgatc tcggactcat cggctactac gccgggcttg acgacaagcg cctcctcctc
     1261 ttcatcctgg gggagccgca cgcgctctgg atggtggatg tcaccaccgg cacccgcaag
     1321 aagctggccg acgacattgg ccgctgtttc cgcccaatgc cgtgggtggg tcccgacgcg
     1381 atcgcttacg tgcagaagga cagcaccggc tcgcagctct gcgagctgga tgtgacgacg
     1441 ggcaaacgcc ggacgttgat gcccatgccc gaccgcacgg tcgaggactt cgagtgggtg
     1501 gacgtcagcg ccagggggcc aacgcggctg gtgctcaccg cgttcgacgg gcgcatcctg
     1561 ttcgcaacgc tgggcgagtc tgatgcctgg aagacctgga tcgacctcac gccataccga
     1621 ttgacggcac tcgcccggct ggcctggagg ccgggtgcgg acttcgggtt gttcgtcgtg
     1681 gcggccgata cgttggcggc tcctaaataa ctgcgtaaca atagtttagg gtgacacaat
     1741 ccgctggccg acggtggctc cccctactcg caccggtgcg ggtccgcacc ccgattgtca
     1801 cgcccaaatc tttccgaacc caacacttga ccccgctgtt caccccggcg ctacactcgt
     1861 accggtacac cccccgcacg atcaggaggc tgtcgcatgc cacggagcga gcgttccaga
     1921 acccccctca gggcgctcgg attgggcgtc ggtcgatgtc ttgttttcct gttgatggtg
     1981 atcgcgggat ctcccgcgat tgcgcgcgcg caggggccga acgatccccc ggattcggcg
     2041 gtcgtgcgcg tggcggtgag gaccgacaag caggtctacg agccgggcga gccgatccgg
     2101 atcacggtca ccgcaacgaa tccgctcgat cgcgcgatca cgctcacctt caactccacg
     2161 ctgcaggccg actacggatt cgacaatgcc tggcagtggt cggatcatcg cgggttcggc
     2221 gacgccatca cgcatgtcac catcaaggcc aacgaaacgt ggaccttccc gtcgttcacg
     2281 cacctcccgg ccgactactt ccctcctccc ggccgtcatg cggtcagggg gatcgtgacg
     2341 gggtacggcg aggcgcaggt gacgatcggc gtggggggca atacgccgcc gccgtcgccg
     2401 ctccatctca cgccggagat cgtgccgaac ccggtgccgt tcggcgtgcc gatcgagatg
     2461 ttcgtcacgg tcaccaatct ctccagcacg acgcagacat tcggtcacag cggctgtcct
     2521 gtgcatttca cggtcgacca gcattactcc cccccggtag cctgcgccga gtactggcgg
     2581 caggtgacgc tcgaggccgg agagtcgatc cggttcgggc cgccggagtt cgattacctc
     2641 acgcttggtc gcgccgagag cggattcgta ctcacgccgg gcgtgcatgt cgtcgatttc
     2701 gaggtgccgg gcgtcgggaa aaccgcggcg cgcatccagg tggcgggcgg gggcggctcg
     2761 atcagcggtt cggtggtggg gcttgacggt cagctgctgc atcacgcggt cgtgcaggcg
     2821 gtgacctacg ccacgcccga cagcacgccc tggaacggcg gcaatttcgt gcatcagacc
     2881 ttcacggacg agcacggcca gtatcgtttc gacgggcttc cgtccgatct ctacttcgtg
     2941 aacgcctcgt acaactcttc gccgccggtc tggtatccgg gtgtgccgac gcgcgacatg
     3001 gccacgccgc tcccggtgtc cgaggggacg aaccaccaga acatcgactt cgcgctgaac
     3061 cagaccgggc cgccgccgcc accgacgaag gtggtgaccg gtacggtgct ggggaatccg
     3121 ccgccgcagt cggaacgctg ttgcccggcg ccactcgcgg gcgcagtggt ggcggccgtg
     3181 gcggaacggg cttcggcggt cgacgacacc atcctcccac cgccacctcc ggacggtggt
     3241 ggctccggtg ggccgccggt gcccggcgat ccgacccgcc cgggtgaacc ctggccggga
     3301 cagcctcccg tgttttacgc cttcgcggac agcgttgggg atttctccct tccgctgccg
     3361 gcggggcgct accgcttcgt ggcattcaac caggggttcc ggtaccagtg gtgggaagcc
     3421 accaccgact ggagtgagtc cgacgtcatc gagatcaatc cgtacacccg cagcctggga
     3481 ccggacctca tgttcacact cgatcctttg cccggcccga atggtggagc gggggcggtg
     3541 atcacgggga ccgtgcatgg cctcatccag gccgacgatc ccgccaccgg tgatccgcgc
     3601 gggcttcctc cgatccagat ggtgccgatc gagggagcgg atgtcgtggc gcgtccgctg
     3661 gtgctcgatc cgcgcatcct ctggttcgtg gaggtgcact acccggtgcg gaccgatgcc
     3721 gacgggcgtt accgcatcga attgccggcc gacctgccgt acgtggttca gacatgggcg
     3781 gcgggctggg agagccagtt ctacgatcat gcctggagcc gcatcgaggc ccggccggtc
     3841 gacgtggcgc cgggtgccga aacgacctcc atcgacttcg acctgcacca ggggtacaag
     3901 ccgcccgatc tcggcgtgat cgccgggagc gtgttccggg ccggcaccga cgacgtgggg
     3961 agcaacaacg acccggcgcg catctatccc gtggggggcg ccaccgtgcg agtgcggctg
     4021 gccgggcccg gcttcggcgg attcgaccga gtggcgatga ccgccgagga tggcacgttc
     4081 cgcctggacg gcctgccgat ggccgacgac ggttcgctca agtacctcgt gtcggccgag
     4141 gccgagggga gcctgcccgc ttatttcccc gacgcgatga gctggcagga ggccacgccc
     4201 ttggcgcccg gaaatgacgg ccagcgctcg ccggtccgca tcgtgctgac cccgcgccag
     4261 gccgagggtg ccttcttcct ggtgggtgtc gtacggggcg atcccggcag cggggtgcct
     4321 cccgtcgagc tgccgccgtg gccgggcgat gacatcgccg acgccgtggc ggactcgagc
     4381 ggcatcccgt ggtggccgtt gatcggcgcc tatctctatc tcgtgccggc ggacgatccc
     4441 tcgcaggggc ccgtggcggg tggcgccacg agcgacaacg gcacgttgat catcccgcac
     4501 ctggcgccgg gccgttacaa ggcctacgcc gatcaccccg gcttccacac cggctggttc
     4561 catggcgcca atcgcgccga ggcggccgtg ctcacggtga ccgatgggga gccgctcctc
     4621 gtggacctcg tgcttgaaac cgccttcccg ccgccgggaa gcggcgggga cggcaacgat
     4681 gccgcggcgc cggccatggt cagcgggctg cgcaattcac cgaacccctt caagccgcag
     4741 accaccatcc agtatcgact tctggcgccg gccgatgttt ccgtgcaggt gtacgacctg
     4801 aacggccggc tggtgcggac cttgatcagc gccgtccacc aaccagccgg ggaacagttc
     4861 ctcccctggg acggcaagga cgaaaccggc gtcgaagcgt cgggtggcgt ttacttctat
     4921 cgggtctgga cgccggtcga aacccgcact ggaaagatgg tgatggtgcg atgagcgttt
     4981 cccacgcgat ccgggcactg accacggcgt tgatgatcgg gcttggcgcc gccggcctct
     5041 ccgggtgcgc ggacgacggc aacaaggagc cgatcgaaac gctggccccc gacatggtgc
     5101 gcctcaccgg ttccgtatcc gagatcgacg atgacgtgcc ggctgacggc ggcgtgacga
     5161 tccggatgac cgacaaggtg aagggtgccg tgacgctcaa tttcgaatcg ctcttcacgc
     5221 tgccgcggcc gaccgaagcg cggcaggccc tgtacgcgga catccagaaa gtttcggcag
     5281 gggatgtcgt gactgccacc gcgcggctgg tggaagggaa ctatcggctc gagagcatcc
     5341 gggtcatcga ggatcactag cggcagccgg gctcggggca ctgctgccgt caactccccg
     5401 tcggcggcgc gccgacgatc ccgcgcgaac ggaggtccgc cagctcgtcc gccgttgtcc
     5461 cgcacagctc ggccaggacc gcgtccgtgt gttccccgag acgtggcggc gccgagcgcg
     5521 gcgcccccgg cgtggcggag aagttgagcg gcaatcccac ctggcggatc gggccgatcg
     5581 tgggatgggg gagcgtgacc accatgtttc gcgcggccgc ctgctccgat tccagcgctt
     5641 ccgaaacctt tcgcaccgcg cccgagggga catcggctgc ggcgaggcgg gcgagccagt
     5701 acgcccgagg gcgggcccgg aagaggggaa gcaggatgtc gtcgagcgcc tcgcgatgcc
     5761 ttacccgatc ggcgttggtc gcgaagcgcg catcgtccgc cagctccgga cgctcgacgg
     5821 cggtgcagaa ggcacgccac tgccccggcg tgccgaccgc cacgttgaac cagccgtcgc
     5881 tcgcggccat cgtggcatag ggcgccagca tcgggtgcag gttgcccatc cgtgccggtt
     5941 cgtggccggc caccagccag tccgcgccgg cgaaaccgag gagcgagagc tggctgtcgg
     6001 ccaatgccag gtcgatttgc tggccggtgc ccgatcgctc gcgcacgtag agtgcggtgg
     6061 tgatgccccc caccatccac atccccgtgg tgatgtcggc ctgcgacacg ccgcacttga
     6121 gcggcggccc atcggcctgg ccggtgagat acatcacgcc accctccccc tgcgtcacca
     6181 ggtcataccc agggcgatcg cgccacgggc cctcctggcc gtagcccgtg atggaggcgt
     6241 acaccagccg cgggttgagc gccgcgcagt cggcgtaacc gaaaccatgc cgctcgagcg
     6301 tgccggggcg gaagttctcg atgaggacat cggcgcgagc caccagtttc cggaggagtg
     6361 cggccccttc cggcttctgg aagtcgagcg tcacgctttc cttgttccgg ttgatgctga
     6421 ggaagtagag gctttcgccg gcagcgaacg gagggccgta ggcgcgtgtg tcatcgcccg
     6481 agagcgattc gaccttgatg acgcgggctc ccatgtcccc cagcaccatc gtgcaccagg
     6541 ggccggccag gacgcgggag aggtcgagga cgaggatccc ctcgagcggc ttcggactca
     6601 ttccccgatg atcttgacca gggcgcgctt cttgcggcgg ccgtcgaact cgccgtagaa
     6661 gatctgttcc cacgggccga agtcgagctt tccggccgtg acggccacga ccacctcgcg
     6721 ccccatcacc tgccgtttca ggtgcgcgtc ggcgttgtcc tcgccggtgt cgttgtggcg
     6781 gtactggtcc accggcgcat ggggcgccag tttctccagc cactcctcgt agtcgtgatg
     6841 cagccccgat tcgtcgtcgt tgatgaagac cgacgccgag atgtgcatgg cgttgaccag
     6901 gaccagcccc tcccggatgc cgctctcggc gagcgcgctc tcgacctcgg gagtgatgtt
     6961 cacgaaagcg cgccgcttcg ggatgttcat ccagatctca cgtcgaaagg tcttcatggt
     7021 ggcggcactc cccggggagg tgatcgggcg catggccctc gagcggcacg cattgtaacc
     7081 cgctacgacc cggcccggac cgcgcgggcg gtgaagctcc ggccgtcgtc gaggcgcgcc
     7141 tggacgaagt agacgccggc cggtgaggtg tccggaagcc accggaagaa ggtctcgccg
     7201 gccgcgacgg cacgcgcgtc ggcgagtagg gccacgcggc ggcccgccac gtcgaacaca
     7261 gtcaccgtga cctctccacc tcggtcgagg cgcagcgcca acgagagcac ctggcggaat
     7321 ggatgggagg cgatcaacag ctcgccgcgg ctcgcgggcg tgtcgggaac ggcggtgacc
     7381 gggcccgggg cgtggtgccc gggcgtgtcc tggcagatga aggtgtcgat cccgccgtcg
     7441 tcgatgcggc gcgtgcgcac ccgctggcgg gtcgaggggg gtcgttcata ctcgtggcga
     7501 ctgtcgtcga attccacgcg ggcggtcgga tcgttcgagt cgacgatcca gcggagatgc
     7561 tggaaatcct cgacatagtt cccgacctgc acgttggacg ccaggaatcc atagtgacgg
     7621 gcccggagat ccacggggag gaaaccgagg ccgtcgcacc agacaccgac ccacggcgcc
     7681 acgcccatca ggttgaacgc ccggtcggcc aggttcacgt attcgccgtc cgggcgcatg
     7741 agcgttccca cgcaggtggg gaatccgagc gcgcgcaggc cggaggcaaa gacatccgcc
     7801 tgtccccacg gatcggagag gcccgcgttc caggcgctgg ccgcgttttc cggcgcccag
     7861 atggagatga ccagccggac atcgatggcc tcgttcacgt aggccatcca ccgctggacg
     7921 acgccgtgct caccgaacgg caagccatcg tcccactggc tcgtttcggc cgcccagcga
     7981 accgcctcgt cgatcccggc cacccatggg atggtgagag aggggctcgt gtaccagtcg
     8041 cccggccggc gcaccggctc cgcgtggagg cctccccgcg tcacgtccga ggaggagtcg
     8101 gtttcctcgc gaatctccac gcgcgcgccc ccattcaagg caccgggggg ccaggtgagc
     8161 cgcgtgtacc actcgctccg gtcctcctcg acgtggggcg gcgatgacag gtaccaggtg
     8221 gtcgtctcct ggttgaacac gccgacgtcg ccgccgggtg acagagcttc gggacgcgga
     8281 agggtcacct cgagccagtc ggttccgggt cggagctcga tcgtccacgt cctcatggtc
     8341 gtgaccgagt tgtgatcgtc gggtccctgg aagtcctcga agcgcgtgcc ttgtccggcg
     8401 aggccgggtg ttggcgcggc caggatcgcg aggagcatca gcgcaagggg cggcgatgcc
     8461 ggcaggatga ccctcatcgc cccgcctcgt cgagtcgcgc gatggccacg cgatgtcccg
     8521 aggcgtcgaa gagcccaacc catcggccga gcgtgtcgac cgtggccgcg tcgaggctca
     8581 gggccagcgt gcgccggagc gggctcgacg catgcaggcg ccaggcattc cagccggccg
     8641 cgtcgcgcca gtcgggcgcg ccgattggat gccggcggcc acccgggtgc cgtggaatga
     8701 tgtccgaatc ggtgggatgg ccggagtcac gaggcaacgc ctcggcgacc gccccctcga
     8761 ttgccgcggc gtccacgaca tcgggggcgc cggcgtcggg ccccaccacc aggtaccagg
     8821 gcgccgatgt cgagagtccg cggccgagcg tggccgtcac ccgccagatg gacgagcgcc
     8881 gcacaccatc gcgcccggca cccgggaaga attcggcgcg ccaggcacga tccactcgca
     8941 aggtgacgcc atcgcgcgcc ggttcgaccg ccggcaaccg caccgacgac atcccgacgg
     9001 tcaggacggc ggcgacacat gccgcgagga gcggaccgcc acgccggtac gggcccggtg
     9061 cccttccggg caattcgagg cgccgcatca tggccggccg ccgaagcgtg acttgatttc
     9121 actccagctg ctgtcgtgcg gccgcttctc gggcggcttc ttgggtgggc gcggcccgcc
     9181 gccgctccgc acggcgccgg gacgaatgcg catgggtgcg gcgaggcggg gcccggcgac
     9241 ctccggcgca tcgacctgcc gcgtggtgac ctgcggcgca tgacacgcgg gcgcttgccc
     9301 ggccggaccg gtgtagcttc cgacgaccag cgccgtcatc acctcgagga aaaaccggaa
     9361 gaacatgaca tctcccttcc ggcgggagat cgggggctcg acacgagggg gtgcgaacga
     9421 tcgggcgggt ccattcaccg tgcgcgccag ggcgccgagt cgtcaagtgc atttcgcgcc
     9481 gcgccggcgg aatcgatttc gtatgatcgt tcgatggtag aaacgcccgg cccacgcccc
     9541 ccgaactgga catcgacacc gatcccggag tttcgccctg acgacgaggc tccgggaact
     9601 cccgtgccgg ggccgatcgt ttatcgacct cccgtccggc cgcagcagca gcccgactgg
     9661 gcgcccccac ccttcggccc gccgattgtc atcaggccga actggccgct ccatatcgcc
     9721 ctcttcctgg ccacgatagc caccacgtgg ttcacggggg gtccgctgta cgccgcctcg
     9781 gtcctggcca tcctcctgtc gcacgagatg ggacactacc tgacggcgct tcattaccgg
     9841 gtgccggcca cgccacccta tttcatcccg atgccattcg ggctgttcgg cacctttggc
     9901 gccgtcattc ggatggcgac gcagggggcc aatcgacgga tcctcttcga cattgccgtg
     9961 gccggcccga tcgccgggat ggtgcttgcg gtgccggcct gtgtgatcgg actccggctt
    10021 tcgcgcgtcg tgacggtcgc caacgcggga gagattcatc tcgggtcgtc actcctcttt
    10081 cagtggctct ccgacctggt cgtggggaag ctgggccccg ggcaggacat cgagcttcac
    10141 ccggtggcat tcgcggggtg ggcgggcctg ttcgtcacgt cgctcaacct gatcccggtg
    10201 gggcaactcg acggcgggca cgtggcctac gcactgctcg ggcggcgcgc ccacaagctg
    10261 gcctacctgg tggcggccgg cttcgtggcg ctcacgattt tcgtgagctg gtcgtggctg
    10321 gtcgtggcgg tcatggtggc cctcttcggc ctgcgccacc ccccgacggg ggacgactgg
    10381 acgccggtcg acacgaagcg catgatcctc ggcgttttcc tgttgttgat gtttgtcatc
    10441 accttcacgc cgcgcccgct ccagtgaggt ttcgcccgga tgcctgaact acccgagatc
    10501 gccacgatgg tgaaggccct caagtcccgg atcatcgggc gggtcgtgac ccactccgta
    10561 ttcgaagagt cgggattgct ggatggcggc tcgcccgagc aggcacggaa ggccatcctc
    10621 ggccgcgaga tcgtcgatgt ggcccggtgc ggcaagtacg tgcgaatcga cttcgccggc
    10681 accatcgggg gggacatcct cgacctgatc gagccgcgac ggaagggggg cgcccggaag
    10741 atgaagccca aggcggcgcc cctgcccccc gatgccagcc tcgtggtgcg ccgcccggcc
    10801 acgctggccg acgcgctggt cctcgccgac ggcgacgact ggattcatgg cgccgccatc
    10861 gacgcgctga ggacgaccga gaagcggggg cacggggcgc cactcacggc gcccgcgcgc
    10921 caacgccttc tcgccgcccg ccggcggcag ttcggccgct acgcctccgg cgaacgctcg
    10981 agcctgctgg tccatctcaa gatgaccggc cgctggtacc tgatgaacga caacggccag
    11041 atgctcccgc cgcgcacgcg tttcgcgatc atgctcacct tcgaggatga aaacctggtc
    11101 ctcgggatga aggacgtccg tcgcctcggc accgcgcggg tgctgacggg tgcggcggcc
    11161 cacgcctggc cggccgacct cgaactcggc ccggacgcgc tcaccacccg gtggaccggt
    11221 aacacgctga agaagcgggt gagtggctcc ctcccaatca agctcgcgct gctcgaccag
    11281 tcacgcctgg ccggcgtggg gaatatctac gcctcggagg cgctccaccg cgcggccatc
    11341 cacccggccc gccccgccga ttccttgacg aacgaggagt ggcagcgcct cgcggtcacg
    11401 atccccgatc tcctccggca ctccatcagg aactggtgcc acctgtcgcg ctgggtcggg
    11461 ccatcggtcg agggctacgg caatttcaac ggggagctcg gcgtatacga ccggcgcggt
    11521 gagtcgtgcc ggcgctgcaa gggaatcatc cactcgatgg tgcagggggc ccgcaccact
    11581 ttctattgcc cggggtgcca ggcctagagg tcaactggcc tgtcgggtgt cgccggtgtc
    11641 gaagcggtcc gccgcccagt cgagcatcga ttcccaatcg cgccgatcga ggggataaat
    11701 cgtctcgttc acgttgaacg ccatccggtc gagcccggcg agcgggaggc tgggaaggag
    11761 tttccgccac tcctcgctca ggcgatcggc cagtgagtca ccgagctcgg aggtgtcagg
    11821 gcagatgatg gccacccgac cttcgcgcac ccagcaggga atgtcgagtt cgcggatctg
    11881 gttcagcacg aaatcggtga gggcgaagcg ggcaccctcc agccagtcgc cgcccgcgct
    11941 ctcggccatc gcccggatcc cttccagctc gagggtgacg agggcgaagg tgcggttgta
    12001 acggtccgcc cgtttcatct cctcggcgat ccgttcctgg aagaccgtcc accgcatgaa
    12061 acggtcggta tccccgccgg ggagctcgcc ggcttccatg cgcaccagcg tctggccgag
    12121 cgaggccgag aggcgcacga gaagggcccg gtcttccgag tccccggcca gggccgttcc
    12181 ctttcgccgg tagaccgaca gcacaccacc cgggatgccc gggatcggaa gcgccagcgc
    12241 ccagcgcact cccgcttcat tccccagtcg ctcttccagg agggtgggac actcgtcgag
    12301 ggagacgacg cgccccccga gggcggcaag ctcgccgaac tcggcatcga ggaggagcag
    12361 gcgttgcccc tcgggatcgc cgatggcgaa gtcggcatcc gagagttcga ggttgtcgga
    12421 gtccgggcgc ctcaggatcg cgaggtcggc atccacgatt tccgcggcgg cctcgacggc
    12481 cagccggttc aattcccccg cggaggttgc cgagaggagt ccgcccgaca ggtcggagag
    12541 gcggagcatc tgccgggtgc gtcgcgtgag gagcacctgc gaccgttcga attcgaggcg
    12601 ggtggtgagc cgtgcggcgg cctgggtgag ggcctcgagg cgttcgctcc agccgggctc
    12661 ctcggccggc aggtcctcca gctcgatgag tcccatcggt tcggcgccgc gcaacggcag
    12721 gagcaggagc gcccgcggcc ccgtcgcgtc gatcgtgctg gtgagcaccg tcgattcgtt
    12781 cctgtccaat gcccgggcag tgaatccgcg atcggccatc acggtgctcc ggcgcgaacc
    12841 gggcgcggcc gagccgaact cggcggccac gtccagacga cggccggtgc catccagcag
    12901 ataaagtcgc ccggccgccg cccgtgtgcc ggtagcgagc ttgtgcagca cggcctcgag
    12961 ccggtgatcc agcggccggt cgagtgtcat cagcgattcc acggcgatct cgaagcttcg
    13021 cgccaggggt cgctcgcccc ccgccggctt cagggcccgc agcagcacct ccgccacctc
    13081 ggagccgaaa cgctccatct gctcgagcga gtgggcggtg aaggcattcg gccgcgagtc
    13141 gctcgacacg ttgaggacgc cgatcgtccg cccggccacc tggatgggcg ccgagatggc
    13201 cgactggatg ccggagcgct cgcgtccgct tcggtatcgc gggtcgtcca gcttgtcgac
    13261 gaggacgcgc gcccggccgt cccgcaacac ctggccggcc acaccctcgc cgcggcgctg
    13321 gctggttttc cggatgacct cgtcggaaag tccgtcggcc accacgatgc gcaggtgctg
    13381 gccactttcg tcgaggagca tgatgctgcc ggaatcgccg ccggccgtgt tcaccgcgat
    13441 ctccaggatc cgcttgagga ggcgttgctg gtcgccggcc aggtcgagcg tttcggaaat
    13501 ccgcttgaga tccccggagg catgcgccgc cgtgtcgaac caggcggggt cggcgatgac
    13561 ttcgagctcg tagtcacgat ggcccgatcg aggagcctcc tcctgcacct ccggcgcgtc
    13621 gacattctcg aatacggcgt caacccgctc gagggggtgc cgctcgatct ggagcggaga
    13681 gtcgtcctcc atgtcccggt gcacctgggc cggtgacgaa gcctgggggg ctgtcgcggt
    13741 ggcggtctcg gcggccgcgg ccgccgccgc ggccggcagg ggaagcatcc aggtgctgga
    13801 cggctcttcc gttgcgctgc cttcggcccg caggtcgagg agcgtggcca tctccgtccc
    13861 ggtcatgatg ccggccagcg ccgatccaag cacccggcac acgtccacct ggaggtcggg
    13921 ccggtcatcc gggatgacga ggaggtcgca gggatagcga cggagcagct cgaggtcgcc
    13981 cgaagcagga atctcgagga tctccgccat cttgaaggcg cacgaggccg gattcgaatc
    14041 cacgacgact tccgggcgga attcgccacg gcgggtgatt tcggagaaga tgtcgagttg
    14101 tcgatcgggc aggcagatga acccgacctt ccgcacggcg gatggcacga tgcaaaactc
    14161 ccggtttggg tttgactcaa cagatcggca ccctcggccg aaaatcgagt caaaagcatg
    14221 tgcggggccg ttgtccctcg agtacaggga ggcggaaaca ggtggatccc gggtgggatc
    14281 gcccgccgac cgagcctata atcccccgtc cacgttccac aggagatcca tgtccgagct
    14341 tcccaccacc accgaaaacc gcgtcgccgt cgtcgaggaa cgacgtatca gcaagcaggg
    14401 gcgcgttttc gtcaatcgca acctccggct gccgagcctc ggcgccatcg gcttcgacct
    14461 cgatcacacc ctggcccact acgcccccat cccggtggag acactggcct tcgacgtgac
    14521 caagcgaaag ctcgtcgaga acaaggggta cccggccgcc atcctcgact atcactacga
    14581 tccccgcttc gtcacccgcg ggctggtcat cgacaagaag cgcggcaaca tcatcaagat
    14641 gaactaccac ggttacgtga gccgcgcgta tcacggccgc aaggagctcc acaccgagga
    14701 ccggaagaag cagtaccggc agcgccccat ccacctctcc aataccggag gctatgcctc
    14761 ggtggacacg ctcttccacc tgcccgaggt gtacctctac ctcctcctcg tggagatgaa
    14821 ccaggccgcc ccgcgcaagg atcgccgctc gttcagccag atctacaccg acgtgcgcga
    14881 gatgatcgac gaggcgcatc gcgacggcac catcaagacc gtcatcgtgg ccgacccggg
    14941 caagtacctg gcgcccgacc cgcgaatggg ccccacgctc gagcactttc gttccgcggg
    15001 gaagaaactc tttctgctga ccaactccga gtacgcctac acggacgtcc tgctgtcgtt
    15061 cctcctgaag cacaacctgg ccggcaagca gagctggcgc gacttcttcg acatcatcat
    15121 cgtggatgca cgcaagcccg atttcttcac ccacaaggaa aacgacgagc ccaggccgca
    15181 tcacgaggag ctccccggcc cccccatctg gtccggcggc ggcgcccgct tcctcgagga
    15241 ccggcttggc taccagggcg atcgcatcct ctacttcggc gaccacacct acggcgacat
    15301 cctccgcagc aagaagtcgg tgggatggcg aaccgccatg gtcatcgagg agatggaacg
    15361 ggaaatcgag gcgctcgaga tgatccgccc gaagatgatc cgcctcggca cgctggccgc
    15421 cctgcgcgag cgcctcgacg ccgacatggc cttcgtccgc cgcgagcgca acaagctggc
    15481 gttgatcgcc gagaacctcg gcggaagcaa ccccgccgtc gtggccaagg tgcaggggcg
    15541 cctgacccgc cgcgaagacg aactggcccg catgcagtcc gagctctcct ccgccatcaa
    15601 ggaaagccgc cagctccgca aggagaacaa cgaagccttc aacagcttct ggggctcgat
    15661 cttccgcgaa ggaaacgaga cgagccggtt cgggcatcag gtggaggact tcgcctgcct
    15721 ctacacggcg cgggtttcca acttcctgaa ttacccggcc tcgcagtatt tccaggcgcc
    15781 gattcggttg atgccgcacg agatttgatt tcccgccgat tttgccgact tttgtcagct
    15841 gatttccatg aatcgcacat gacagccatg ccgggccagt gtgatgccat atcctttcgg
    15901 gacacagcag aggaagcgcg ccggcgacta tcgatcacgg agccttcgat caagggcagg
    15961 ggcattgggt cgcctgccga aaagcacccc gggggatttc catgcggact cgttatcttg
    16021 ccgtactgtt cgggatcgcc gcccttgcac tcctcccctc gatcgctccg gccaatccga
    16081 ttcccggcct ctacaacagc acggacctcg gtggccagct gttgaccggc cgcgcctcga
    16141 cctggcgtcc cgggatcaac agcggactcc cgcatgtcct gcacgcgcag agctgggaca
    16201 gcgccacgct gggcacccag tgggacgtga gttgcgccat cgagaccacg ccgttcagtg
    16261 tccaggacaa ccggatcgcc ggtaccggca ccgtggtcta caccagtacg ttccaggggg
    16321 gcacgctcac cctgtacccg ggcgcctggc cgtgggggga tggcaccgcc acgctcggca
    16381 cgaccacgct catctccacg gtccagttcg tgaacaacat ccccgtggcc agcgtcgtga
    16441 acggcaacac gagcggcgag ttcaccaatg gctgcaacct ggtcttcgcc atcggcaacg
    16501 gcagcggcgt cggcgaaacc acctcgctca atcccctgat caccaagccg gccaccttcc
    16561 ccggcttcct ggacggtacc tgtggcccgg ccggccccgt cgtccagttc ggcacgtggg
    16621 gcaccaccct cacccagacc atggccatcg actgcggcgc cctgccggtc gagccgtcga
    16681 cctggagcgc ggtgaagggg cttcgcctcg actagatcga tttccgaacg acaccttcgg
    16741 cccggcagct cccgcgttgg agttgccggg ccgttctcct gacgaagcgt ccttgttttt
    16801 ccgcaacttg ttttatttta tgacccatat aaaataacgg ccacaccgct cactgaaagg
    16861 aacatcccat gctcctcgcc gccctcaccg ggttcctgct caccggcacc gtgaaccagt
    16921 tcggtcagtc gtaccgtttc gagctggcgg cggcttccgg cggcgccttc gacttcagga
    16981 aggaaggacc ggcgggggag cgactggtgt gggatgggtc gtcgggttgg cgcgtcgatg
    17041 ccgatgggcg ggtcatgcca ctcgaattat tcgacctgga gcagacgcgg ctcaccgcgt
    17101 ggctggcggt tggttcgacg ctgggttggc gggcgaatgt cagtgccgac acgctggtcc
    17161 ttccgggggg gcgcctggtg actcgggtga cgagaaacag cgacggtgat atcgcgctgg
    17221 ccacctggcc gggggtgtcg ggggaagttg cctggagctt cgactggacg acggcggcgg
    17281 acggcgtgcc cgacgtggcc gcgcgatgga cccagcgcgt gggcgacctt gtcgtggaga
    17341 ccatgagcgt caccgacgtg aaccggaatg ccgagttcga cccgagccga ttcgccgagc
    17401 cgcctcgccc ggccgacgat gccatctggc ttcccgaggc gccggacacc ctcgagatca
    17461 tgcgggccca gaccggccac ctcctcatcc gtccccgcct gaacggcagg gacgttggct
    17521 ggttcatctt cgacacgggt gccggcgaat cggtcgtcac gcccgccgtg gcagcgcagc
    17581 tcggattgcc cgaagtcggc cgcacggtcc tcacgtcgat cctcggctcg gccgaatccc
    17641 ccgtctacgg cgtcgacacg ctcgatgtcg gaaggctccg gctggcccat cgccggttga
    17701 cggccatgga cctggctccg ttcgaaggac ctctcggcgt tcccctgtcc ggtgtcctcg
    17761 gcggcgaagt gctctcccgc gccatcctca cggtggaaac ggccgtgccg cgactcacgg
    17821 tgcagaaacc ggacgaagtg acagtcgatg cggccgcctg gcggccgctc catttcaacc
    17881 agcgccttcc gctcatcacc gccgacttcg cggggggcac gggcgagctc ttccggatcg
    17941 acgtcggcgc ggggggaagc ttcggccacg tggccttcca ttcccccgcg gtggcccggc
    18001 tgggactcct ggccggaaac gactcggagc gcctcgaagc cgctggcatg acctgcgtct
    18061 tcgcgccgat cgcctggttc aacctggccg gtcatcgctt cgagaacgag ccggtcgtgt
    18121 acgtcgaaaa gtccgaggga ccgatggccg actcctgggt gaccggcaac atcggcgtgg
    18181 agctgctcaa gccgttccgg gtgatcttcg attgcccgaa tcgccgcatt gccttcgtgc
    18241 cgcgatgatc ggtaagcgaa aggggaggcc ggtgggccgc cgcatcacga ccgcgctgct
    18301 gtcgcggccg gcgcaggcca aggcgctgag ccagcccatc cggctcgaga tcgtggaggc
    18361 cttccggccg ggcgaggaac tcggcgtgga cgagctggcg gcccgcctcg gtcgcaagcc
    18421 ggtatcgctc tactttcacg tgcggaacct cgtggccaat gacattctcg tggccgccga
    18481 tgagcgccgc ggagccggcc ggccgacacg gctctaccgg gtggccgccg atcgactcat
    18541 ggtgtcgccc tccccgaagg cctcgcgcga catacaggcc gggttcgatc tcttccgtgc
    18601 caccctccgc gccaccgaac gcgacttccg caaggcgctg gccctctacg ccaggcccgg
    18661 ctcggtccag ccgacacgga tggcggggca gcggatgaaa gggcgcctca gtgatcggga
    18721 gctgaagatc gtcgagggcc tgctcacccg gctcgaaaac atcttcatca acgccaccgg
    18781 ccggcgctcc gggcgcctgg tggccgtgac gacggtcatg gttccgatcg ggtagattga
    18841 cccaccgtga cgacatccgg gtgagt
//
LOCUS       DASVOD010000205        12463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_325958, whole genome shotgun sequence.
ACCESSION   DASVOD010000205 DASVOD010000000
VERSION     DASVOD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12463
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325958"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VF720_04380"
     CDS             <1..1469
                     /locus_tag="VF720_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7878618.1"
                     /translation="VARSLTAIRDFDHLLDVVLDTAIRELSAEMGILVLLNAAGEAVN
                     RAARNFAEESLPEDQVQVSRKVLRNVVESGKPACITDALSDPDWISESVQGLSLRSIL
                     CVPLVLRDGVGGALYLENRRVAGIFGPREQRYLEAIASLAAIGLDNAHLYAELENRRE
                     ALQDENARLKIALGTPENFEGFVGGAPAMQRIFHLLKRVADSKVNVLIRGESGTGKGL
                     VARRIHELSPRRDGPYVEVNCAAVPETLLESEFFGVAKGTATGVDARVGRFEQASGGT
                     IFLDEIGDMSLVLQAKILKAVQERIVERVGGRGPTTVDVRIVAATNKNLEEAVAKGEF
                     REDLFFRLNVVPIVLPPLRDRMSDVPALADFFLKRHAKENDRAARGFTRAAMDLFMAH
                     TWPGNVRELENAVAQAVVLSEGTRIETHDLPPSLRDGGRKGGPRTLDDATAHAEKDLI
                     VAALEEHGWSRQVAAKALGVSRVTLYKKMKRYGIEAGD"
     gene            1462..3300
                     /locus_tag="VF720_04385"
     CDS             1462..3300
                     /locus_tag="VF720_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878619.1"
                     /translation="MTDTAGRRLAIIAIALPIVLGLIVRLLAWPHQSIVTVDGTSYIR
                     LARDLMGGAEYLTVQPPGYPVMMMPFLLGADVEGVLAARVTALLAGIGAMLLFGLLAR
                     RLVSRWGWLAFGLAFATTPILVRYSIVTMSEMPYLLWTAAALLAASHGRWWLAGAFGA
                     AAFHVRPEGMFLVGGLALAAGRRPRAWLGLFSGLVLLGFLPAMIYNHAQSGSWSLTLK
                     DTNIVADTPVQNESIRAVERDPAKPDTGLTARLSQFAGVSLRVWPGRFVAEMKNMARA
                     VGWPWLVAALAGLFLVIRRSTRPAAPPGPTDPDAAIADPRPPPPGRLLAAGLIQLLVV
                     PLFGGVAPVPRLIVPVLPFVLLLGLIAIDNLVRRDRRLGAIAAMAAAAAWFVAALPEA
                     KALRLQEDGYYPELVRAGRELRGILPAEALVMDRKPYTAFYAGTRFEQVPFGSYHATI
                     EAIQRMGGDYLVVDEAVAEVFRPDLMPLVGDSFTMLNEARLQPIWFDPSLQRRHTAVY
                     RIRRRDERAGPPADDAASSNAQGSGSPIPPGREAAVRDLMARVPHQPKLHVLHAEILW
                     LAGRRAEALAEYEAARAAGTFRPVDAERVRLLEAEIAAGDSATAGR"
     gene            complement(3284..3712)
                     /locus_tag="VF720_04390"
     CDS             complement(3284..3712)
                     /locus_tag="VF720_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878620.1"
                     /translation="MNRTTRSYPGGRPDSGRPFLPFVAPVLLLAGALALASCSTDFDD
                     DEAREHQTILIKQGASMPQENQSFSPNPVTVLVKTEILWVNQDSLAHQIASVGALFPA
                     ATRPIPPGGEFTVLLKDQGAYRYYCLLAGHREEGVINVLP"
     gene            3932..5284
                     /gene="thiC"
                     /locus_tag="VF720_04395"
     CDS             3932..5284
                     /gene="thiC"
                     /locus_tag="VF720_04395"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX7878621.1"
                     /translation="MQPRSNGASPHDNVTQMHFARKGVVTPEMEHVATREAMDVELVR
                     SEVARGRMIIPANVNHASLEPMAIGIAAKCKINANIGNSSTTSDVEGELTKLHAAVHF
                     GADTVMDLSTGGDIDAIREAIIAKSPVPIGTVPIYQALQEVPRVEDLTAEKLLDVIER
                     QAKQGVDYMTIHAGVLAEHVPLTRGRITGIVSRGGSILAQWMVGHHKQNPLYERFDDI
                     CEIFKKYDVSFSLGDGLRPGCLADANDEAQFAELATLGELTQKAWKHDVQVMIEGPGH
                     VPMHLVEMNVKKEMAVCHEAPFYLLGPLVTDIAPGYDHITSAIGAALAGWAGAAMLCY
                     VTPKEHLGLPDANDVREGVIAYKIAAHAADVARGRKGARDRDDELSKARFDFDWERQF
                     ALSLDPERARSMHDATLPDEAYKSAKFCSMCGPKFCSMRITQTLRKLEDVEAAAGAGH
                     "
     gene            5311..5691
                     /locus_tag="VF720_04400"
     CDS             5311..5691
                     /locus_tag="VF720_04400"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HEX7878622.1"
                     /translation="MAAGVIRRGDYVLIARRLPHAHLGGFWEFPGGTREEGESLEECL
                     VREIQEELGLTVRVGMHALTVRQDYPDRALDLHFYLCTPVAGTPEARGCAEFRWVTVG
                     ELTGFEFPPADIPLIEQLQAVPGH"
     gene            5688..6938
                     /locus_tag="VF720_04405"
     CDS             5688..6938
                     /locus_tag="VF720_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878623.1"
                     /translation="MNIERLLAPDYAAHPILDPDWLEKDPEGAGALRQALSEMSAGPE
                     NYNAHLNRLLRPWDDPRALNQNLPVIHSYLREVDLYLRELLQLVPPDAAGKLAPLAAV
                     TGTNDLKHLLDLVFTADGKRARFEAQRKIGLARLFFDVDHTDDIQHGPAHTRALEECL
                     ARELWAHVTRARRFTVSFSLTPDGETIRYRLGPPEKGEDSWPVHLRRIARPVGGRPAH
                     VHVFYHSCRFKRRYRFRTVEPLAPQEIDPDDVPIWEELKRGRSGSIVSKMIRKGEIDP
                     QKIGDLIGAMFIVKDAAEVERLQAMLYDIFGGPFRWREAVDTIKSHDDRRRLHEQSAG
                     GFQVKKSIVGVLHRPDDAGKPPYIFNVEFQIYTLEGFLKTVHSRHEASHERMKLRQFL
                     EGLLPYLFPAGIYGEETLKSCLGK"
     gene            complement(7593..8942)
                     /locus_tag="VF720_04410"
     CDS             complement(7593..8942)
                     /locus_tag="VF720_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7878624.1"
                     /translation="MVGDLMPVGLLPARFRRPAITVALIVAATIVCGCGDERSLSPSR
                     PAEETSPSASAGLIVVGAPAEVVVVLRRSTVPSAVWEDYGLAPQEVLSDGRTWRTVVQ
                     GSGRSVGDILTALAGDTRVNDAEPNVPVGTPLLKQSSMSFDEGFPSIESYHDQSVNRR
                     LHLDLAHATSRGYGALVAVLDTGVDLDHPGLRNVLVDGYDFVDRDDFPAEAEDGLDQD
                     ADGLVDEALGHGTHVAGLVSLVAPGAKIMPIRVLNNDGVGNAYNVASAIELAVNKGAT
                     VINMSLTFSSPSAAVDRAITYAQQHDVVMVVAAGNTGSYGPLEYPASDPRVLSVASVN
                     SANQRSTFSAWSNTVTLAAPGENLLSTFWNGGYAVWSGTSMSAPIVSGTAALLRSRRI
                     GLSATEVRNRLVLRAHPLNESGYGMGAGIVDPAAAVGFTAPGNAPEFRRIDSARESR"
     gene            complement(9080..11842)
                     /locus_tag="VF720_04415"
     CDS             complement(9080..11842)
                     /locus_tag="VF720_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX7878625.1"
                     /translation="MKRSPPAADLLARVAEANRTDPRQSMRLVRQALAAIPAGRDPEA
                     RATKARLIRYRAHARRALNDFPGALADYRAALAAFRSLGDRHEEAITRIGQVTALFYR
                     GRHTEALAAARAGRSLLVRLGDALRVARLDTNTGNIYHRLDQPLVALTWYERARRSLK
                     RIGDEADLALVELNRGNALTQLGRLDEARTAYRTARSEFAARGQELATAEADYGLAYL
                     LFVENRFTDALAMFDDLKPRLDRLGERRLAALTDMDAAETNLRLHLWEEAEIRAERAG
                     RVFRLLGMRYEEARSGAFLGTALFQLGRKSRALTAWRRSARLFEREGNRTWQGMIFLV
                     MARAERHTGRRAAAHVLVRRALTLVKPPGPPVAAAEARYLDGILSAELATSAKARAHA
                     RTILAGASKEARRLGAEWLRRDAEEALGELARLDGRPTEARKHLTLAVESGERLRSLI
                     AGDDVRAAFFRDRSHPYLSLARLELDAGRLDEAYGWIERGRARSLLTDVGRLPRRPAA
                     ARSKGADRSAELESILRRLSTHYQPGQALVAGQRPAAGAARLPDTLRRHLESRAESLM
                     DRLYPGGRPLPGKSAPAQARRSLLGPDEALISWFETDGVLSAFVETRDGLSLASDLAS
                     VEVVAGAAERLRWQWGRFRLGSRHFERHHRLLVDDAVEDLALLHSLLVAPLVTAAGKN
                     RWIVVPGPGMADLPFAAFHDGRRFLVENRELAVTPARAVLAMCRARLGRRGSGTLLVG
                     QTGAGTPEVEAELEAVRQSATDRVDLLTGAEATVANVRRLAPGRRVLHLASHGFFHAE
                     RPRLSGIRLADRWLHANDVAGQTFDAELVVLSACQSGTSMVFGGDEWMGLPRAFLKSG
                     AARVLASLWDVDDRATRELMSRFATALAVSGTLPASALAAAQRDLLLENRHPYFWAGF
                     ELLGAP"
     gene            complement(11851..>12463)
                     /locus_tag="VF720_04420"
     CDS             complement(11851..>12463)
                     /locus_tag="VF720_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878626.1"
                     /translation="LDFVEQRLGVDDARAIDGHLVGCDSCRSAAGRVAQLLTHLAGMR
                     TDRPPDHLSAWARSIPALVAPKRRPGWLELVADSWGDLTQAIDSALETLQPTPMLRGV
                     DIPHRAGRRRLLFAAGRFDLDLEIAYPGLADPRRIRGQLLPVTDPRQLWTGSDVRLMR
                     GRAVVATARLDARGAFVLPKVAAGRYALSLAGPVPARTPAFEV"
BASE COUNT         2061 a         4136 c         4384 g         1882 t
ORIGIN      
        1 aggtggcgcg gtcgttgacc gccatccgcg atttcgacca cctgctggac gtcgtcctcg
       61 acacggccat ccgcgagctt tccgccgaga tggggatcct ggtcctcctg aatgccgcgg
      121 gggaggcggt gaaccgggcc gcgcgcaatt tcgccgagga gagccttccc gaggaccagg
      181 tgcaggtgag ccggaaggtg ctgcgcaacg tggtcgagag cgggaagccg gcctgcatca
      241 ccgacgcgct gtcggatcct gattggatat cagagtccgt ccaggggctg agccttcgtt
      301 cgatcctctg tgtgccgctc gtcctgcgtg acggcgtcgg cggagccctc tatctcgaga
      361 accggcgtgt ggcgggcatc ttcgggccgc gggagcagcg ctacctcgag gccatcgcat
      421 cgctggcggc catcggtctc gacaacgccc atttgtacgc cgagctcgag aaccgtcgcg
      481 aagccctgca ggacgagaat gcccgtctca agatcgccct gggcacaccg gaaaacttcg
      541 aggggttcgt gggcggcgca cccgccatgc agcggatctt ccacctgctg aagcgggtgg
      601 cggacagcaa ggtcaacgtc ctgatccgtg gcgagagcgg caccggcaag gggctggtgg
      661 cgcgccgcat ccatgagctc tcgccccgtc gcgatggacc ctatgtcgaa gtcaattgcg
      721 cggccgtccc cgaaacgctg ctcgagagcg agttcttcgg cgtggccaag ggtacggcca
      781 ccggtgtcga cgcgcgcgtg gggcgttttg aacaggccag cggcggcacg atcttcctcg
      841 atgaaatcgg cgacatgagc ctcgtgctgc aggcgaagat cctgaaagcg gtacaggagc
      901 ggatcgtgga acgggtgggg gggcgcgggc cgaccacggt ggatgtccgg atcgtggccg
      961 ccaccaacaa gaacctggag gaagcggtgg cgaaagggga gtttcgagag gacctcttct
     1021 tccgactcaa cgtggtgccg atcgtcctgc cgccgctccg cgaccggatg agcgacgttc
     1081 cggcgctggc cgatttcttc ctgaaacgcc atgcgaagga aaacgatcgg gctgcccgcg
     1141 gctttacccg ggccgccatg gatctcttca tggcccacac ctggccgggc aatgtccgcg
     1201 aactcgagaa cgccgtggcg caggcggtcg tcctctccga gggaacccgc atcgaaaccc
     1261 acgaccttcc accgtctctc cgcgatggcg gcaggaaggg aggcccccgc acgctggacg
     1321 atgccaccgc ccacgccgag aaggacctga tcgtggccgc gctcgaggaa catgggtgga
     1381 gccgccaggt ggcggccaag gcactgggcg tgagccgcgt cactctctac aagaagatga
     1441 aacggtacgg gatcgaggcc ggtgactgac acggcgggcc gccggctcgc catcatcgcg
     1501 atcgccctgc cgatcgtgct cgggctgatc gttcgactcc tcgcctggcc ccaccagtcc
     1561 atcgtgaccg tcgacggcac cagctacatc cggttggccc gcgacctgat ggggggggcc
     1621 gagtacctca ccgtccaacc ccccggatat cccgtgatga tgatgccgtt cctgctcggg
     1681 gcggacgtgg agggagtcct ggccgcccgg gtgacggcgc tcctggcggg gatcggcgcc
     1741 atgctcctgt tcggactgct cgcacgccgg ctcgtctcgc gatggggatg gttggccttc
     1801 ggcctggcgt tcgccaccac gccgatcctc gtgcgctatt cgatcgtcac gatgtcggag
     1861 atgccctatc tcctctggac ggccgcggcg ctgttggccg cctcgcacgg ccggtggtgg
     1921 ctggcgggcg cgttcggtgc ggccgcgttc catgtgcggc ccgaggggat gttcctcgtg
     1981 ggagggcttg ccctggcggc aggccgtcga ccgcgagcct ggctgggcct tttcagcgga
     2041 ctcgtgcttc tgggatttct tccggcgatg atctacaacc atgcgcagtc cgggagctgg
     2101 agcctgacgc tgaaggacac gaacatcgtg gccgacacac ccgtgcagaa cgagtcgatc
     2161 cgtgccgtcg agcgtgaccc cgcgaaaccg gataccggcc tcaccgccag gctgtcgcag
     2221 ttcgctggcg taagcctgcg cgtgtggccc ggacgattcg tcgccgagat gaagaacatg
     2281 gctcgggccg tcgggtggcc gtggctggtg gccgcgctgg cgggactgtt cctggtgatt
     2341 cgtcgatcga ctcgtccggc agccccgcct ggcccgaccg atccggacgc cgcgatcgcc
     2401 gacccgcgac ccccacctcc aggccgcctc ctcgccgccg gcctcattca actcctcgtc
     2461 gtgccgctgt tcggcggcgt ggcgccggtc ccacgcctca tcgtgcccgt tctgccgttc
     2521 gtactgctgc tcggcctgat cgccatcgac aacctggtcc ggcgggatcg ccggctgggc
     2581 gcgattgcgg cgatggcggc cgccgcggcg tggttcgtcg ccgcgttacc cgaagcgaaa
     2641 gcgctcaggc tgcaggaaga cggttactac cccgagctcg ttcgggcggg gcgggagctg
     2701 cggggcatcc tgccggccga ggcgctggtg atggaccgga agccctacac cgccttctac
     2761 gccggtacgc ggttcgaaca ggtgccgttc gggagctacc acgccacgat cgaggcgatc
     2821 cagcgcatgg gaggggacta cctggtggtc gacgaagcgg tggccgaggt cttccggccc
     2881 gacctgatgc cgctggtcgg cgactcgttc acgatgctga acgaggctcg actccagccg
     2941 atctggttcg atccctcact gcagcggcgg cacacggccg tgtaccggat caggcgtcgg
     3001 gacgagaggg cggggccgcc ggccgatgac gcggcatcat cgaacgcgca aggctccgga
     3061 tcaccgatcc caccgggacg cgaggccgcc gtgcgggacc tcatggcgcg ggttccgcac
     3121 cagccgaagc tccatgtgct tcacgcggaa atcctctggc tggccggccg acgcgcggag
     3181 gcgctggcgg agtacgaagc cgcgcgggcg gccggaacgt tccggccggt ggatgccgaa
     3241 cgggtgcgtc ttctggaagc ggaaatcgcg gcgggggact cggctacggc aggacgttga
     3301 tgacgccttc ctcgcgatgt cccgccagaa ggcagtagta ccggtaggct ccctggtcct
     3361 tcagaaggac ggtgaactcc cctcccggag gaatcggccg cgtggccgcc gggaagagag
     3421 cgcccacgct ggcgatctgg tgcgccaggc tgtcctggtt cacccacagg atctccgtct
     3481 tgaccagcac cgtgacgggg ttgggactga acgactggtt ctcctgcggc atggaggcac
     3541 cctgcttgat caggatggtc tggtgttcgc gggcctcgtc gtcgtcgaag tcggtgctgc
     3601 aggaagccag ggccagcgcg ccggccagga ggagcaccgg ggccacgaag ggaagaaagg
     3661 ggcgcccgga atcaggacga ccacccggat aggaccgtgt cgttcggttc attgcgtgga
     3721 actccagttg ggacaatcgg ggaacgcccc ggaggggagg cgggcaaaga aaaacatttt
     3781 ttccccctcc tgtccagacg gagggcccca tgggcccggt gtccgtgcgg cggcattcgt
     3841 tgacactccc ggatgatcgt gacagcataa ccggcgtagc ggatggcgat tagccggtcc
     3901 gttcccaccc aggaatcgga gattccaagc catgcagcca cgcagcaacg gcgcgtcccc
     3961 gcacgataac gtgacgcaga tgcatttcgc ccggaagggc gtagtcaccc ccgagatgga
     4021 gcatgtcgcc acccgcgaag ccatggacgt cgagctcgtc cggagcgaag tggcccgcgg
     4081 ccggatgatc atcccggcca acgtgaacca cgcgagcctc gagccgatgg ccatcgggat
     4141 cgcggcgaag tgcaagatca acgcgaacat cggcaactcg tccaccacgt cggacgtgga
     4201 aggggagctc acgaaacttc acgcggcggt ccacttcggt gccgacacgg tgatggacct
     4261 ctcgacgggc ggcgacatcg acgccattcg cgaggccatc atcgcgaaga gcccggtgcc
     4321 gatcggcacg gttcccatct accaggccct gcaggaagtc ccgcgcgtgg aagacctgac
     4381 ggccgagaag ctgctcgacg tcatcgagcg ccaggcgaag cagggcgtgg actacatgac
     4441 gattcacgcg ggcgtccttg ccgagcacgt gccgctgacg cgcgggcgga ttaccgggat
     4501 cgtgagccgg ggtgggtcca ttctggcgca gtggatggtc gggcatcaca agcagaaccc
     4561 gctctacgag cggtttgacg acatctgcga gatcttcaag aagtacgacg tgtccttcag
     4621 cctgggcgat ggcctccgtc ccggctgcct ggccgacgcc aacgacgagg cgcagttcgc
     4681 cgaactggcc acgctcggtg agctcacgca gaaggcctgg aagcacgacg tgcaggtgat
     4741 gatcgagggg cccggccacg tgccgatgca cctcgtcgag atgaacgtga agaaggagat
     4801 ggcggtctgc cacgaggcgc ccttctacct cctggggccg ctcgtcaccg acatcgcgcc
     4861 cggctacgac cacatcacct ccgccatcgg cgcggcgctg gccggttggg ccggggcggc
     4921 catgctctgc tacgtgacgc ccaaggagca tctcggcctg ccggatgcca acgacgtgcg
     4981 cgaaggggtc atcgcctaca agatcgcggc gcatgcggcc gacgtggccc gcggccgcaa
     5041 gggggcccgc gaccgggacg acgagctctc gaaggcgcgc ttcgacttcg actgggagcg
     5101 ccagttcgcc ctgagcctcg accccgaacg cgcccgcagc atgcacgacg ccaccctgcc
     5161 ggacgaagcc tacaagtcgg ccaagttctg ttcgatgtgc gggccgaagt tctgttcgat
     5221 gcgcatcacc cagacgcttc gcaagctcga ggacgtggaa gcggccgcgg gagccgggca
     5281 ctgagcgaca gccagcgaca gcgcctcgaa gtggccgccg gcgtcatccg ccgcggtgac
     5341 tatgtgctca ttgcccggcg gctgccgcac gcccacctcg gcggcttctg ggagtttccc
     5401 ggcggcacgc gcgaggaggg ggagtcgctc gaggagtgcc tcgtccggga aatccaggaa
     5461 gaactcgggc ttaccgtgcg cgtcgggatg catgccctga ccgtccgaca ggactatccg
     5521 gaccgggcgc tcgatctcca cttctacctc tgcacgccgg tggccggcac gcccgaggcc
     5581 agagggtgcg ccgagttccg ctgggtgacc gtgggggagc tcaccggctt cgagttcccg
     5641 ccggccgaca ttcccctcat cgagcagctc caggcggtac ccgggcattg aacatcgaac
     5701 gactgctggc gcccgattac gcggctcacc cgatcctcga tcccgactgg ctggagaagg
     5761 atcccgaggg agccggtgcg ctccgccagg ccctctcgga aatgagcgcc ggcccggaaa
     5821 actacaacgc gcacctgaat cgccttctcc gcccctggga cgacccgcgc gccctgaacc
     5881 agaacctgcc ggtgatccac tcctacctgc gcgaggtcga tctctacctg cgcgagctcc
     5941 tgcagctggt gcctccggat gctgccggca agctcgcgcc ccttgcggcg gtgaccggca
     6001 ccaacgacct gaagcacctc ctcgacctgg tcttcaccgc cgacgggaag cgggcgcggt
     6061 tcgaggcgca gcggaagatc ggcctggcgc gcctcttctt cgacgtggac catacggacg
     6121 acatccagca tggcccggcc cacacgaggg cgctcgagga gtgcctggcc cgggagttgt
     6181 gggcgcacgt gacccgggcg cgccggttca ccgtaagttt ttcgctgact cccgacggcg
     6241 aaacgatccg ctatcgcctg ggcccgccgg aaaaaggcga agattcatgg cccgtgcatc
     6301 tgcgccgcat cgcccggccg gtcggggggc ggccggcgca tgtccacgtg ttctatcact
     6361 cgtgccggtt caagcgacgt taccgcttcc gcaccgtgga gcccctcgcg ccccaggaaa
     6421 tcgaccccga tgacgtgccg atctgggagg aattgaagcg cggtcgcagc ggctcgatcg
     6481 tgagcaagat gatccgcaag ggtgagatcg atcctcagaa gatcggtgac ctgatcgggg
     6541 ccatgttcat cgtgaaggac gcggcggagg tggagcggct gcaggcgatg ctctacgaca
     6601 tcttcggcgg cccgttccgc tggcgcgagg ccgtggacac catcaagagc cacgacgacc
     6661 ggcgccgcct ccacgagcag tcggccggcg gcttccaggt gaagaagagc atcgtggggg
     6721 tgctgcaccg cccggacgat gcggggaagc ctccctacat cttcaacgtg gagttccaga
     6781 tctacacgct ggaggggttc ctcaagacgg tgcacagccg ccacgaggcc agccacgagc
     6841 ggatgaagct ccgccagttc ctcgaggggc tgctcccgta cctctttccg gcgggaatct
     6901 acggcgagga gacgctcaaa tcctgcctgg ggaaatgagg cgggaggggg gacgcccgcc
     6961 tgaaggaggg gccggcgggg gcaggaacat cgctgctcgt gtcactcacc gttgccggtc
     7021 tgtcgatcac cggccccaag aatagagagc ggcttccatc ccaccatggc ggtcgggaac
     7081 tccgcagcgg agcaggtcaa gttgccgctc acgatgtcgc tgcggagaaa cggccgagag
     7141 caggtcggca gccgtctctg aaaacgattc actgtctgcg atgcacggtc gattcgctgc
     7201 gacgcacgaa ttactgcctg cgatgcgatg aattcgggcc tggccccggg aggcaccgag
     7261 ttcccggggc cggaccgcga atcgccgaag agcacccgag ggggtttgac gcggaattcc
     7321 ctcaccctcg ccggatgagg ccccccggac gaccgggaag cagttgccgt agcgtcgaac
     7381 actctggccg cgacggaacc caccccgggc gcattcggcg ggaggatcgg tcgccctgag
     7441 ccgaggaagc cgttcccccc ttcatccatg aatgtggggg gaccggcggg caaaatgcag
     7501 gaatttgagg tgacgatccg atgaaatcgt gccggcggcc gggcgtggct cgggtgccgc
     7561 acgggttgcc tggagcggag tgccccgtgg ggctagcggg attcccgggc ggagtcgatc
     7621 cggcggaact cgggggcgtt tccgggggcg gtgaacccca ccgccgccgc gggatcgacg
     7681 atgccggctc ccatgccgta gcccgactcg ttgagcgggt gggcccgcag gaccaggcgg
     7741 ttcctgactt cggtggccga gaggccgatc cggcgcgacc ggagtagcgc cgcggttccg
     7801 ctgacgatcg gggcggacat cgatgtgccg ctccagacgg catagccgcc attccagaag
     7861 gtgctgagga gattctcgcc gggcgcggcg agggtgaccg tgttcgacca ggcggagaac
     7921 gtcgaccgct ggttggccga attcaccgag gcgacggaga ggacgcgcgg gtcggacgcc
     7981 ggatactcga gcgggccgta gctgccggta ttgccggcgg ccaccaccat gacgacgtcg
     8041 tgctgctgcg cgtacgtgat ggcccggtcg accgccgcgg agggggagga aaaggtgagg
     8101 ctcatgttga tgaccgtggc ccccttgttg acggccaatt caatcgcgct ggccacgttg
     8161 taggcatttc ccacgccgtc attgttgagg acccggatgg gcatgatctt ggcgccgggc
     8221 gccaccagcg agacgaggcc cgccacgtgg gtgccgtgtc cgagcgcctc atccaccagg
     8281 ccgtcggcat cctggtcgag gccatcctcc gcctcggccg ggaaatcgtc ccggtcgacg
     8341 aaatcgtagc cgtcgaccag cacgttccgg aggccgggat ggtcgaggtc gacgcccgta
     8401 tcgaggacgg ccaccagcgc gccgtacccg cgactggtgg catgggccag gtcgaggtgg
     8461 agccggcggt tcacgctctg gtcgtggtac gactcgatcg acggaaatcc ctcgtcgaac
     8521 gacatgctcg attgcttgag gagcggggtg cccaccggaa cattgggctc ggcatcattc
     8581 acacgcgtgt cgcccgcaag tgcggtgagg atgtctccca cggagcgacc tgatccctgg
     8641 acgacggttc gccaggtgcg tccgtcggag agcacttcct gcggcgcgag gccgtagtct
     8701 tcccagaccg cggacgggac cgtgctgcgg cgcagcacga ccacgacttc ggccggggcg
     8761 ccgacaacga tcaggccggc gctggccgag ggactggtct cctcggcggg gcgggaaggg
     8821 gagagggagc gttcgtcgcc gcagccgcaa acaatcgtcg ccgccacgat cagcgcgacg
     8881 gtgatggccg gtcgacggaa tcgcgccggc aggagaccga cgggcatgag atcaccaacc
     8941 atgcgggaca cgacgttgac tcctctgctg ctcgaagcgt ggcgcgttga cactcagacc
     9001 cccctgagcc aaccggcagg cgcggtggat tccgccgcct ccgtagtgta ggcgaacatt
     9061 tttccgctcg ccagaaaact cacggagccc ccaacaattc gaagccggcc cagaaatagg
     9121 ggtgtcgatt ctcgaggagc aggtctctct gggccgccgc cagtgcgctg gccggcaggg
     9181 ttccggatac ggcaagagct gtggcgaaac gggacataag ttccctggtg gcgcggtcgt
     9241 cgacatccca gaggctcgcc aggacgcgcg cggcgcccga cttgaggaac gcccgcggga
     9301 ggcccatcca ctcgtctcct ccgaaaacca tggaggttcc gctttggcag gcacttagga
     9361 cgacaagttc cgcgtcgaac gtctggccgg ccacgtcgtt ggcgtgaagc caacgatcgg
     9421 ccagccggat gcctgagagg cggggccgct cggcatggaa gaatccgtgg gacgccagat
     9481 gaagcacccg gcgccccggg gccagccggc ggacattcgc cacggtggcc tcggctccgg
     9541 tgaggaggtc cacccggtcg gtcgccgact gccggacggc ctccagctcg gcctccactt
     9601 ccggggttcc cgcccccgtc tgccccacca ggagggtgcc cgaaccgcgc cggccaaggc
     9661 gggcccggca catggccagg acggcccgcg ccggcgtcac cgccagctcc cggttttcga
     9721 cgaggaatcg acgaccgtca tgaaacgcgg caaaggggag gtcggccatt cccggtcccg
     9781 gcacgacgat ccagcggttc ttcccggccg cggtgacgag cggcgcaacc agaagcgagt
     9841 ggaggagggc gaggtcttcc acggcgtcat ccaccagcag gcgatgatgg cgttcgaaat
     9901 ggcggctccc cagccggaat cgcccccact gccagcggag ccgctccgca gcgcccgcca
     9961 ccacctcgac cgaggcaagg tccgacgcca gcgagagccc gtcacgggtc tcgacgaagg
    10021 ccgacaggac gccatccgtc tcgaaccatg aaatgagcgc ctcatcgggt ccgagcaacg
    10081 acctcctggc ctgcgccggc gcagacttcc cgggcagggg gcgcccaccc gggtagaggc
    10141 ggtccatcag tgattctgcc cgcgattcga gatgtcggcg cagcgtgtcg ggcaaacgcg
    10201 ccgccccggc ggccggacgc tgcccggcaa cgagcgcctg tcccggctgg tagtgcgtcg
    10261 agaggcggcg caggatcgac tcgagctccg ccgatcggtc ggcgcccttc gaccgggccg
    10321 cggccggcct gcggggaagg cgtccgacgt cggtcaggag ggagcgggcg cggccgcgct
    10381 cgatccagcc gtacgcctcg tcgagacggc cggcatccag ctccagacgg gcgagcgaga
    10441 ggtacggatg cgagcggtcg cggaagaaag cggcccggac gtcatccccc gcgatgagcg
    10501 aacgaagccg ttcgcccgat tcgacggcca gggtgagatg cttcctggcc tcggtcggcc
    10561 ggccatccag gcgggcaagt tcgccgagcg cttcctcggc atcgcgacgg agccactcgg
    10621 cgccaagccg acgcgcttct ttcgatgccc cggccaggat ggtgcgcgcg tgcgcgcgcg
    10681 cctttgccga cgtggccagt tcggccgaca ggatgccgtc gaggtaacgc gcttccgcgg
    10741 cggccaccgg cggacccggt ggtttgacga gggtgagagc acggcggacc agcacatgcg
    10801 cggcggcccg acgcccggta tggcgttcgg cccgcgccat gacgaggaag atcattccct
    10861 gccaggtgcg gtttccctcg cgctcgaaga gccgggcgga tcgccgccag gccgtcaggg
    10921 cccgggactt tcgcccgagc tggaagagcg ccgtcccgag gaacgcaccc gatcgcgcct
    10981 cttcatagcg catgccaagc agccggaaga cacgaccggc gcgctccgcg cggatttcgg
    11041 cctcctccca gaggtgaagc ctgagattgg tctccgccgc atccatgtcg gtgagcgcgg
    11101 cgagtcgcct ctcgcccagc cggtcgagcc gcggcttcag gtcgtcgaac atcgcgagcg
    11161 catcggtgaa gcgattctcg acgaagagaa gataggcaag tccgtagtcg gcttccgcgg
    11221 tggccagctc ctgcccgcga gcggcgaatt cgctgcgggc cgtgcggtag gctgtgcgcg
    11281 cctcgtccag ccggcccagc tgcgtgaggg cgttgccgcg attcagctcg accagcgcca
    11341 ggtcggcctc gtcaccgatg cgcttgagcg atcgacgggc gcgctcgtac caggtgagcg
    11401 ccacgagtgg ctggtcgagc cggtgataaa tgttgccggt gttcgtgtcg aggcgcgcca
    11461 cgcggagcgc gtcgccgagc cgtaccagga gcgagcgacc ggcccgtgcg gcggcgagcg
    11521 cctccgtatg ccgcccgcga tagaaaagcg cggtgacctg cccgatgcgg gtgatggctt
    11581 cttcatgccg gtcgccgagg gagcggaagg cggcaagcgc cgcccggtaa tcggccagtg
    11641 ccccgggaaa gtcattgagg gcgcggcggg catgggctcg atagcggatg agccgcgcct
    11701 tcgttgcgcg tgcctcggga tcccgaccgg ccgggatcgc ggccagtgcc tggcgcacca
    11761 gccgcatcga ctggcggggg tcggtccgat tggcttcggc cacccgggca aggaggtcgg
    11821 cggccggcgg tgaccgtttc acgggcacgc tcagacctcg aaagccggcg tacgggccgg
    11881 aacggggccg gccagcgaca gggcatagcg tcccgccgcc actttcggga gaacgaaggc
    11941 gccccgcgca tcgaggcgcg cggtggccac gacggcgcgc ccccgcatga ggcgtacgtc
    12001 gctgccggtc cagagctgtc gtggatccgt gacggggagg agctgcccgc ggattcgtcg
    12061 cggatcggcg aggccgggat aggcgatttc caggtccagg tcgaaccggc cggcggcaaa
    12121 gaggagccgc cgccgaccgg cacggtgggg gatgtcgaca ccgcgcagca tcggcgtcgg
    12181 ctggagagtt tcaagggcgg aatcgatcgc ctgcgtcagg tcgccccagg agtcggccac
    12241 gagttcgagc caaccggggc gtcgtttcgg tgccacgagg gccgggatcg accttgccca
    12301 ggcagagagg tggtccggcg ggcgatcggt gcgcatcccg gccaggtggg tcaggagctg
    12361 ggccacacgg ccggccgcgg aacggcagct gtcacagccg accaggtggc catcaatggc
    12421 ccgcgcgtcg tcgaccccga gccgctgctc gacaaagtcg agc
//
LOCUS       DASVOD010000206         9494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_378200, whole genome shotgun sequence.
ACCESSION   DASVOD010000206 DASVOD010000000
VERSION     DASVOD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378200"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VF720_04425"
     CDS             complement(<1..541)
                     /locus_tag="VF720_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878627.1"
                     /translation="MTLIRSSAALMIMILVPVIQTGCSQRSPEPPAAQAGEHASVETR
                     HAARDVEPGKAMSLKEAYPDYHPGADDPEADAARKGRREVPVSDRAFDIGGAPSLNDL
                     VNLVVSGVDQAEGPTLERTAITRHDFETILWPEFPQSRPAAGVPSAEAWDFVHRQHVA
                     SFNRTAGELHGKGLRVASMK"
     gene            complement(556..3705)
                     /locus_tag="VF720_04430"
     CDS             complement(556..3705)
                     /locus_tag="VF720_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7878628.1"
                     /translation="MRNRLLRSLLVILAIVGASAPAVAGSFPDSTAAPAPALRIIEEG
                     NGVVRGRLTKTETGEPLAWAYVTLTRLDQADAVKILTSNADGYFSGEVAPGHWSVLVE
                     HLLWPAKKIDDVEVRPSEALVLDLSLTDRTMVEYRGHVVDGLSPTENVSQANVLFQPV
                     PIDASVPPFGAVTGADGRFSVRLPTGVYEVSVLALNYARTVVSRVAISPGINPALHLE
                     MLPEAKVTVQEINVRGNRITSTAVSELQTRRLSAAVTDGISRETIRKGTDADAAQVLG
                     RVTGVSVKDGKFLVVRGMSERYTSTQVNGVRVGSPETNRKVVPMDIFPASLLDNVVLQ
                     KTWTPDLPGDFGGASVQITTREVPETPVFSSSFGAGMRSGTTGEDGLGYTGGALDFLG
                     IDDGSRGLPDLVSAMAGNQRIVQKSPLRPDGPGFSKDDFLKLARSFNPEWSVSDEPPP
                     LNSLWNMAVGRRMDVMGRPLGFVGALAYQNTYVTRSGDVFNYEDNTLSTVKGDYDEVR
                     TTRSVLWGGIGNLSYRLGESSSVKLNTLYTLSSDDEARRYEGYYLDIDTDIRVERLQF
                     QQRDLFSGTLAGQHGLPVLHSRIEWKLNRSDAHRDEPDRRENFYAKTTSQILEESENG
                     STVRDTTYWGFSGRGADRLFSYLDDQEMGTALDWTVPFRSWADGDARLRMGWATNDKD
                     RVSWTRRFTYRPLNGIDLGEPVDSVLANDHIGNGMTFTENTRGTDYTWADQSIDAWYL
                     VADLPVQKRMRLSAGARLEKASQGVSYYSTDVFTSDILKTEHVTDDFLPGVNVTWAPG
                     ATQNIRASWSKTVNRPDLRELSPTEFTEYESGASSIGNPGLRRALITSYDLRWEAFPT
                     LGELLSISGFYKKLTDAIESNIQGGEFPRKQPVNTPEGRNIGVELEARLGLGRLSSKL
                     QRFGAGANLVLVDSEVSFGGSDGVQTSRVRPLTDQSGVVGNLALFYTGPRSEASVYFN
                     HFGRRLTDVGVYGQPDVYEEGRLAIDAAFSQRLGRSLKLKLVAKNVTNSTQLKTQGGR
                     VREEFATGAGYGLSLSFGD"
     gene            complement(3702..6707)
                     /locus_tag="VF720_04435"
     CDS             complement(3702..6707)
                     /locus_tag="VF720_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878629.1"
                     /translation="MTLGRLARTVSLALFLAAPAMAQDIVWDGNISFNNNPNGCNDAA
                     TGGFLEDNVLSGQFTHNRFVDPQLVNAASLTNPDFRPVAGGNAICGTHPVIALPADGF
                     FDQVNYAGALSPNAADNWATGAWISYDPNGNPAEFNLGKPLITLTAGNLPANYVFSSA
                     NNYLIEGRVSVANGRNCTIQPGTVIFGSTTVTPSYFVIERGGKLNANGRPNQPIIFTS
                     ELAPSNTAAPGNWGGVVWHGRACANCANTAAGDSCVSEGNAGSYGGTDDTYDGGSMTY
                     TRIEFAGFQVAPNNELNCLTMNALGDRTLIDFVQTHQGSDDLFEWFGGTVRHKYLIGT
                     HGDDDGLDFQMGYRGKVQFAIIQQEPNTAANTVDKGIEGDNNEFNFANPLCRSNPTFA
                     NLTLVGVRTTDGPATTSQGGPGIHFRRGARGEVLNSIVMGFKACGFDLDDAETVAGGF
                     GPNGTPICPAEVKSPVITWDGNVLFDNNPNGCNDAATGGVLENTVLFGTPFTHNRQVN
                     PNLTNAASLSAPSFVPLPTSNALCSNFHPVVTLKSDGFFTQTSYAGALAPDASQDWTT
                     GWTTFDPNGNPADFNLAKPLVTLTAGNLADGYAMTSNNNYVIEGRVAVANGRTLVIQA
                     GTAIFGSTTVTPSYLVIERGGQVIANGTRQAPIIFTSELAPSGTAAPGNWGGFVWHGR
                     ACANCANTAAGDSCVSEGNAGSYGGIDDEYNGGSLSFTRIEFAGFQVAPNNELNCLTM
                     NALGQGTSVNWIQTHQGSDDLFEWFGGTVRSKYLLGTHGDDDGLDFQMGFRGRVQFAV
                     IQQEPNAAANTVDKGIEGDNNEFNFANALCRSNPQFANLTLIGVRTTDGPATTSIGGP
                     GVHFRRGARGEVLNSIIQGFKSCGFDFDDAETVAGGFGSNGIVYCSGTSGVIADGGSG
                     AGALSVRAFPNPVRQSSRISFSLPSAARTTVKVFSLSGQLVSTLADEEMPAGSHEMDW
                     VPENLSDGIYFYAVDAGNLKATGKLVYVR"
     gene            6975..7871
                     /locus_tag="VF720_04440"
     CDS             6975..7871
                     /locus_tag="VF720_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX7878630.1"
                     /translation="MRLLVSGPTGLIGTPLVERLEREGYEVRRLSRSPGSGRVTWDPA
                     TGRLAPDAFDGVHGVIHLAGESIAGGRWTPARKGAIRNSRVAGTTLLARTAAAARPLP
                     RFFISASALGYYGDRGDALLDETAPPGTDFLAQVCQEWEAAATPARDAGIRTVAVRTG
                     LVLAPEGGALKEMLLPFRLGVGGPLGNGRQYWSWITLDDIVAAFDHAIRTDTLEGPVN
                     GVTGAVTNRDFTRVLNGVLKRPGFIPAPAAALKIMLGEMAEPLLLASARLVPRRLRDS
                     GFRWNDPELEPALRRLLGRSPR"
     gene            7940..8401
                     /locus_tag="VF720_04445"
     CDS             7940..8401
                     /locus_tag="VF720_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878631.1"
                     /translation="MEHLYSAALLLHLLAYAAWVGGGVAQQRFMSASRRAGLEAVIRD
                     QYERLSAALLTKLELPAALVSIVSGLVILWLRPGLLKMPGMHMKLTLVALILVLTHLE
                     MFNARRIVRLRESGGDLGAIADRKSRHAAYGTVGGLMVLGILVIVAFVIRG"
     gene            complement(8441..8908)
                     /locus_tag="VF720_04450"
     CDS             complement(8441..8908)
                     /locus_tag="VF720_04450"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7878632.1"
                     /translation="MGDTLRKRIQQSRFESPLQEAILNILVAAGHIRERVNRLCAVHD
                     ISNTQFNVLRILKGGPAEGYPRHEIQERMLERAPDVTRLIDRLEVRGLAERGRSDSDR
                     RLSVTRITPAGVRLLEELQPHIGKLMTELSTRLDEQDCHELSRICEKLYSDDE"
     gene            complement(8988..9362)
                     /locus_tag="VF720_04455"
     CDS             complement(8988..9362)
                     /locus_tag="VF720_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEX7878633.1"
                     /translation="MAAERAKKLERKTRPTPALEARAGRGLPDPDPVIHERLRLRILS
                     TLAVQPNLTFNDLKGILDATDGNLSVHARRLEEAGYLDCTKEFAGRVPRTRYRITPAG
                     RRALERYLEQMENLIRAARGTG"
BASE COUNT         1443 a         3089 c         3278 g         1684 t
ORIGIN      
        1 ctttcatcga ggccacccgt agccccttcc cgtgaagctc gccggcggtc cggttgaacg
       61 aggccacgtg ctgccggtgg acgaaatccc acgcctcggc cgaaggaacc ccggcagccg
      121 gccggctctg gggaaattcg ggccagagga tcgtctcgaa gtcgtggcgg gtgatggcgg
      181 tccgctcgag ggtgggtccc tcggcctggt cgacgccgga taccaccagg ttcacgaggt
      241 cgttgagcga cggggcgccc ccgatgtcga aggcccggtc gctgaccggc acctcgcgac
      301 gccccttcct ggcggcatcg gcttcggggt catccgcgcc cgggtggtaa tccgggtagg
      361 cctccttgag cgacatcgcc ttgcctggtt ccacgtcacg ggcggcatgc cgggtttcga
      421 ccgaggcgtg ttcgccggcc tgggcggcgg ggggttcggg tgaacgctgg ctgcaccccg
      481 tctggattac cgggaccagg atcatgatca tgagagcggc ggacgaacgg atgagagtca
      541 caaaggctcc tttggtcaat cgccgaaact gagggagagg ccgtagccgg cgccggtggc
      601 gaactcctcg cggacccgcc ccccctgcgt cttcagctgc gttgaattgg tcacgttttt
      661 cgccacgagc ttcaacttga ggctgcggcc gaggcgttgc gagaaggccg catcgatggc
      721 caggcgccct tcttcataga catcgggctg gccgtacacg ccgacatccg tgaggcgtcg
      781 gccgaagtgg ttgaagtaga cggaggcctc gctgcgcggc cccgtataga agagggccag
      841 gtttccgacg acgcccgact ggtcggtcag ggggcgcacc cgcgaggtct gcacgccgtc
      901 cgacccgccg aagctcacct cgctgtccac gaggacgagg ttggcgcccg ccccgaagcg
      961 ctggagcttc gaggagaggc ggccaagccc gagcctggcc tcgagttcga caccgatgtt
     1021 gcgcccctcc ggcgtgttga ccggctgctt gcgggggaat tcgccaccct ggatgttgct
     1081 ctcgatggcg tcggtgagtt tcttgtagaa gccgctgatc gacagcagtt ccccgagggt
     1141 ggggaacgcc tcccagcgga ggtcgtacga cgtgatgagc gcgcggcgga gccccgggtt
     1201 gccgatgctc gaggcgccgc tctcgtactc ggtgaattcg gtcggcgaga gctcgcgcag
     1261 gtcggggcgg ttcacggtct tgctccacga ggcgcggatg ttctgcgtgg cgccgggagc
     1321 ccaggtgacg ttgacgcccg gcaggaagtc gtccgtcacg tgctcggtct tcaggatgtc
     1381 gctggtgaag acgtcggtcg agtaatacga aacgccctgg ctggccttct cgaggcgcgc
     1441 gccggccgac aggcgcatgc gcttctgcac cggcaggtcg gccaccaggt accaggcgtc
     1501 gatcgactgg tcggcccagg tgtagtcggt gccgcgggtg ttctcggtga aggtcatccc
     1561 gttgccgata tgatcgttgg ccaggaccga atcgaccggc tctcccaggt cgatgccgtt
     1621 gagggggcgg taggtgaagc ggcgggtcca ggagacgcgg tccttgtcgt tggtggccca
     1681 ccccatccgc agccgcgcat ccccatcggc ccacgagcgg aacggcaccg tccagtcgag
     1741 cgccgtaccc atctcctggt catcgaggta gctgaagagg cgatcggcgc cgcgcccgga
     1801 gaaaccccag taggtggtgt cgcgcacggt cgatccgttc tcgctctctt ccaggatctg
     1861 cgacgtggtc ttcgcgtaga aattctcgcg tcggtcgggc tcgtcgcggt gggcatcgga
     1921 tcgattcagt ttccactcga tgcgcgagtg caggaccggg agaccatgct gccccgccag
     1981 cgtgcccgaa aacaggtcgc gctgctggaa ctggaggcgt tccacgcgaa tgtcggtgtc
     2041 gatgtcgagg tagtacccct cgtagcgtcg ggcctcgtcg tcgctgctca gcgtgtaaag
     2101 cgtgttcagc ttgaccgagg aactttcgcc gagccggtag ctcaggttgc cgatgccgcc
     2161 ccagagcacg ctccgggtgg ttcggacctc gtcgtaatcg cccttcacgg tggagagtgt
     2221 gttgtcttcg tagttgaaca cgtctcccga gcgcgtcacg taggtgttct ggtaggccag
     2281 tgcgcccacg aagccgagtg gccggcccat cacgtccatc cgtcgcccca ccgccatgtt
     2341 ccagaggctg ttcaggggag gcggttcatc gctcacgctc cactcggggt tgaacgaacg
     2401 ggcgagcttc aggaagtcgt ccttgctgaa accgggaccg tccggcctga gcgggctctt
     2461 ctgcacgatg cgctggttgc cggccatcgc cgacaccagg tcgggcaatc cgcgcgagcc
     2521 gtcgtcgatg ccgaggaagt cgagtgcgcc gcccgtgtag ccgagtccgt cctcccccgt
     2581 ggtgcccgag cgcatccccg cgccgaacga cgacgagaac accggcgtct ccggcacctc
     2641 gcgggtggtg atctgcaccg aggcaccgcc gaaatcgccc ggcaggtcgg gggtccaggt
     2701 cttctgcagc accacgttgt cgaggaggct ggccgggaag atgtccatcg gcaccacctt
     2761 gcggttcgtc tccgggctcc ccacccggac gccgttgacc tgcgtggagg tgtaacgctc
     2821 gctcatcccc cgcaccacca ggaacttgcc gtccttcacc gacacgccgg tgacgcgtcc
     2881 gagcacctgc gcggcgtccg cgtcggttcc cttcctgatg gtttcacggg agatgccgtc
     2941 ggtgacggcg gcggagaggc gacgcgtctg cagttccgac acggccgtcg aggtgatccg
     3001 attgccgcgg acgttgattt cctggacggt gaccttggcc tcgggaagca tttcgaggtg
     3061 gagtgccgga ttgattcccg gcgaaatggc cacgcgcgag acgacggtcc gtgcgtagtt
     3121 gagcgccagc accgacacct cgtacacgcc ggtggggagc ctcacgctga agcggccgtc
     3181 tgcccccgtt acggcgccga acggcggaac cgaggcgtcg atcggcacgg gctggaaaag
     3241 aacgttggcc tggctcacgt tctcggtggg gctgagccca tccaccacgt ggccacggta
     3301 ctcgaccatc gtccggtcgg tgagcgagag gtcgagcacc agggcttcgg aggggcgcac
     3361 ttccacgtcg tcgatcttct tcgccggcca gagaaggtgc tccaccagca cgctccagtg
     3421 ccccggagcc acctcgccgg aaaagtatcc gtccgcgttg ctggtgagga tcttgaccgc
     3481 gtcggcctgg tcgagccgcg tcaacgtcac gtaggcccac gccagcggtt cgccggtttc
     3541 ggtcttcgtg aggcggcccc ggacaacgcc attcccctct tcgatgatcc gcaacgccgg
     3601 ggccggggcc gctgtcgaat cgggaaacga cccggcgacc gccggagccg aagctccgac
     3661 gatcgccagg atgacaagca aggaacggag aagacgattc ctcatcggac gtagaccagt
     3721 ttccccgtgg ccttcaggtt gccggcatcg accgcataga agtagatgcc gtccgacagg
     3781 ttctcgggca cccagtccat ctcgtgcgaa ccggccggca tctcctcgtc ggccagcgtg
     3841 gagacgagct ggccgctgag cgagaagacc ttgaccgtcg tgcgcgcggc gctggggagc
     3901 gagaaggaga tccgggagct ctgtcgcacc gggttcggga aggcccgcac cgagagcgcc
     3961 ccggcaccgc tgccgccgtc ggcgatgacg cccgacgtgc cggagcagta cacgatgccg
     4021 ttcgatccga atccgccggc caccgtttcg gcgtcgtcga agtcaaagcc gcacgacttg
     4081 aagccctgga tgatggagtt gagcacctca ccgcgggcgc cgcggcggaa atgcacgccc
     4141 gggccgccga tgctggtggt ggcgggtccg tcggtggtac gcacgccgat cagcgtgagg
     4201 ttggcgaact gggggttcga acggcacagg gcgttggcga agttgaactc gttgttgtct
     4261 ccttcgattc ccttgtcgac cgtgttggcc gccgcgttcg gttcctgctg gatgacggcg
     4321 aactgcaccc gaccgcggaa gcccatctgg aaatcgaggc cgtcatcgtc gccgtgcgtg
     4381 ccgagcaggt acttgctgcg gaccgttccg ccgaaccact cgaagaggtc gtccgaaccc
     4441 tggtgcgtct ggatccagtt cacgctggtc ccctgcccga gggcattcat ggtcaggcag
     4501 ttgagctcgt tgttcggcgc cacctggaag ccggcgaatt cgatgcgggt gaagctgagc
     4561 gagccaccgt tgtactcgtc gtcgatgccg ccgtacgagc cggcgtttcc ttcggacacg
     4621 cacgaatcgc cggcggcggt gttggcgcag ttggcgcacg cccggccgtg ccacacgaag
     4681 ccgccccaat ttccgggagc tgccgttccg gacggggcca attccgaggt aaagatgatc
     4741 ggagcctgcc gcgtgccatt ggcgatcacc tgcccgccac gctcgatcac caggtacgac
     4801 ggtgtcaccg tcgtcgaccc gaaaatcgcc gtgcccgcct ggatgaccag cgtccggccg
     4861 ttggccaccg ccacgcgccc ctcgatcacg tagttgttgt tgctggtcat cgcgtagccg
     4921 tcggccaggt tgccggcggt gagcgtcacg agcggcttgg ccaggttgaa gtcagcggga
     4981 ttgccgttcg gatcaaaggt cgtccatccg gtcgtccagt cctgcgaagc gtccggggcg
     5041 agtgcgccgg cgtagctggt ctgggtgaag aatccgtcgc tcttcagggt gacgaccgga
     5101 tggaagttgg agcagagggc gttgctggtc ggcagcggca cgaagctcgg tgccgacagg
     5161 ctggcggcgt tggtgaggtt cggattgacc tggcggttgt gggtgaacgg cgtgccgaag
     5221 agcacggtgt tctcgaggac accgccggtg gccgcgtcgt tgcagccgtt cgggttgttg
     5281 tcgaacagca cgttgccgtc ccaggtgatg accgggctct tcacctcggc ggggcagatc
     5341 ggcgtgccgt ttggcccgaa accgccggcc acggtttcgg cgtcgtctaa gtcgaagccg
     5401 cacgccttga atcccatcac gatgctgttg agcacctcgc cccgcgcgcc gcggcggaag
     5461 tggatgcccg ggcccccctg gctggtggtg gccgggccat cggtcgtgcg gacgccgacc
     5521 agggtcaggt tggcgaacgt ggggttcgag cggcagaggg ggttggcgaa attgaactcg
     5581 ttgttgtcac cctcgatccc cttgtccacc gtgttggcgg cggtgttcgg ctcctgctgg
     5641 atgatcgcga actgcacctt gccgcggtac cccatctgga agtcgaggcc gtcgtcgtca
     5701 ccgtgggtgc cgatcaggta cttgtggcgg acggtgccgc cgaaccactc gaagaggtcg
     5761 tccgagccct ggtgggtctg cacaaagtcg atgagcgtgc gatcgcccag cgcgttcatg
     5821 gtgaggcagt tgagctcgtt gttcggggcc acctggaagc cggcgaactc gatgcgggtg
     5881 taggtcatcg atccgccgtc gtaggtgtcg tcggtgccgc cgtagctgcc ggcgttccct
     5941 tccgacacgc acgaatcgcc ggcggcggtg ttggcgcagt tggcacaggc gcgtccgtgc
     6001 cacacgaccc caccccaatt gcccggcgcg gccgtgttgc tcggcgccag ttcggacgtg
     6061 aagatgatcg gctggttcgg ccgcccgttg gcgttcagct tgccaccgcg ttcaatgacg
     6121 aaatacgacg gcgtcaccgt cgtcgatccg aagatgaccg tgccgggctg gatcgtgcag
     6181 tttcgcccgt tggccaccga gacgcgccct tcgatgaggt agttgttcgc gctgctgaac
     6241 acgtagttgg ccggcaggtt gccggcggtc agcgtgatca gaggcttgcc caggttgaac
     6301 tcggccgggt tgccgttcgg gtcgtagctg atccaggcgc cggtggccca gttgtcggcc
     6361 gcgttcgggg agagcgcgcc ggcgtagttc acctggtcga agaagccgtc ggcgggcagg
     6421 gcgatgaccg ggtgcgtgcc gcagatggcg ttgccccccg ccaccgggcg gaagtcgggg
     6481 ttcgtcaggc tggccgcgtt gaccagctgc gggtcgacga aacggttgtg cgtgaactga
     6541 ccggagagaa cgttatcctc gaggaaacca ccggtggccg cgtcgttgca gccgttcggg
     6601 ttgttgttga agctgatgtt cccatcccag acgatgtcct gggccatggc cggagcggcc
     6661 aggaacaggg ccagcgagac ggtccgtgcc agtcttccga gcgtcatccg aatcctcccg
     6721 atcgcggtgc gtttgcggag tgcctgggcg acggacatcg ccggcacgag tgcgcgggag
     6781 aaaacgtagc gatcgcgatt gtcggtggga tgaagcgtgt gtggcgtcat ggcgacggac
     6841 atgttgccgc gcgttcatga cgcttcggtg acggcggcgg ggatgcggcg tggagattcg
     6901 acggcggtcg cctcgactca tggtggttct gaccacggga cgctcacccc gcgagtcagc
     6961 taagctcgat caccatgcga ctcctcgttt ccggccccac cggcctcatc ggcacgccgc
     7021 tcgtcgagcg gctcgaacgg gaggggtacg aggtgcgccg cctctcccgg tcgccggggt
     7081 cggggcgcgt cacatgggat ccggccaccg gccgcctcgc gcccgacgcg ttcgacggcg
     7141 tgcacggcgt catccacctg gccggcgaga gcatcgcggg aggacggtgg acaccggccc
     7201 ggaagggagc catccggaac agccgggtgg ccggcaccac gctcctcgcc cgcaccgcgg
     7261 ccgccgcgcg accacttccc aggttcttca tctccgcctc ggcgctcggc tattacggcg
     7321 atcgcggtga tgccctcctc gacgaaaccg ccccacccgg cacggacttc ctggcccagg
     7381 tctgccagga gtgggaagcc gccgcgactc cggcgcgcga cgcgggcatc cgcaccgtgg
     7441 ccgtgcgcac cggcctcgtc ctggcgccgg agggaggcgc gctcaaggag atgctccttc
     7501 ccttccggct cggagttggt ggaccgctcg ggaacggccg ccagtactgg agctggatca
     7561 cgctcgatga catcgtggcc gccttcgacc acgcgatccg caccgacacc ctggagggcc
     7621 cggtgaacgg ggtgacgggc gccgtcacca accgtgactt cactcgcgtg ctgaacggcg
     7681 tcctgaaacg cccgggcttc atcccggcac ccgctgcggc gctgaaaatc atgctcgggg
     7741 agatggcgga gcccctgctc ctggccagcg cgcgactcgt tccgaggcgc cttcgcgact
     7801 cgggcttccg ctggaacgac cccgaactcg agcccgcgct gcggcggctc ctcggtcgtt
     7861 caccccggtg agcgggacgc acccccgctg agaaggtcgc accttatacc ggcgctcact
     7921 ccctgctagg atgccgcgca tggaacatct ttacagcgcg gcactcctgc ttcacctgct
     7981 cgcttacgcg gcgtgggtgg gtggcggcgt ggcccagcag cggttcatgt cggcctcgcg
     8041 ccgggccggt ctcgaagcgg taatccgcga ccagtacgag cgcctctcgg ccgccctcct
     8101 cacgaagctc gaattgccgg cggcgcttgt gtcgatcgtg agcggcctcg tcatcctctg
     8161 gctccgcccc ggactcctca agatgcccgg catgcacatg aagctcactc tggtggcgct
     8221 catcctggtg ctcacgcatc tcgagatgtt caacgcccgg cggatcgtgc gcctgcggga
     8281 atcgggcggg gacctgggcg ccattgccga tcgaaagagc cggcacgcgg cctacggcac
     8341 ggtgggcgga ctcatggtgc tggggatcct cgtcatcgtg gcgttcgtca tccggggcta
     8401 gtcgccctgg aacgccaacc gctcgtagcc ctgccggccc ttactcatcg tcggagtaga
     8461 gcttctcgca gatccggctg agctcatggc aatcctgctc gtcgagccgg gtggagagct
     8521 cggtcatcaa cttgccgatg tggggctgca gctcctcgag gaggcggacg cccgccggcg
     8581 tgatgcgcgt gaccgacagg cgacggtccg agtcggaacg cccccgttcg gcgagaccgc
     8641 gcacttccag ccggtcgatc aggcgggtca cgtcgggtgc gcgctcgagc atgcgctcct
     8701 ggatttcgtg gcgcgggtac ccctcggcgg gtccaccctt gaggatgcgc aggacgttga
     8761 actgcgtgtt gctgatgtca tggacggcgc acagccggtt gacgcgctcg cgaatatgcc
     8821 cggccgccac caggatgttg aggatggctt cctgcagcgg actttcgaat cgactctgct
     8881 ggattcgctt gcgaagtgtg tcacccatac aggggttagc atactcccct tgaatcgaat
     8941 cgaataccac ccgaatcaac cgggcggatc catcggcagg gccgatgtca gccagtgcct
     9001 cgagccgccc ggatcaggtt ttccatctgc tcgaggtagc gctcgagggc acgccgcccc
     9061 gccggcgtga tcctgtagcg ggtgcgcgga acgcgaccgg caaactcctt ggtgcagtcg
     9121 agatagccgg cttcctcgag gcgccgggca tgcacgctga ggttcccgtc cgtggcgtcg
     9181 aggatcccct tcagatcgtt gaaggtgagg ttgggctgga cggccagcgt gctcaggatc
     9241 cggagccgaa gcctctcgtg tatgacggga tcagggtcgg gcaggccacg gccggcccgc
     9301 gcctccaggg ccggcgttgg ccgcgtcttc cgctcaagct tcttcgcgcg ctcagccgcc
     9361 atgccggctc ccgatcacga ggccgagcgc caggtgcaat ccgccgaaac cgagcgccat
     9421 gagtgcgtct ccgatggccg ggctcaattc cagcgcggcc gatcccgatc ccgccgcgcc
     9481 gaggagcatg aaag
//
LOCUS       DASVOD010000207         6308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_326083, whole genome shotgun sequence.
ACCESSION   DASVOD010000207 DASVOD010000000
VERSION     DASVOD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326083"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VF720_04460"
     CDS             <1..1305
                     /locus_tag="VF720_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 51"
                     /protein_id="HEX7878634.1"
                     /translation="REATRHSVNLVYIRLMRDIVRHYTTEIPDFDSEMYENRSHPLRR
                     RYLEKFADREGKQFLARFLPRYRGKTSEEILDRVVEEIRPTPSRLAVIFRSVKPAGSV
                     DEFRRWVARVWPAGLGTERGESALRDAYQRYPRTAFDIHDRGYISGLHPLELWLVQYL
                     YLNPTAGWSDLVAASTEERQGVYKWLFNSKSLDGQNNRIRILIEEEAFKKVHAAWRKQ
                     GYPFSSLVPSYATSIGSSADRPAALAELMGIIVNGGVRFPLTRIDTMHLAEGTPYEIL
                     YEHHPMEGKRVYPAELTSVVRKAITDVVENGTARRVYGAFKRADGSVIPVGGKTGTGD
                     HRYDTYAPGGRLIESRVVNRTATFVFLIGDHFFGTLTAYVAGPKAAGYAFTSSLPVQI
                     LKTLAPSLEPLILSDEPGPTQAAAVSSAGADSLHRPIDPHAE"
     gene            complement(1964..2770)
                     /locus_tag="VF720_04465"
     CDS             complement(1964..2770)
                     /locus_tag="VF720_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEX7878635.1"
                     /translation="METGIKGKVAFITGAAHGQGRASALALAAEGAHIAAYDIARTTA
                     YPGYALGQEDDLKSLAAACEAGGVRCLTFAGDVRDDAAITRAVETTVNELGRIDVLFN
                     NAGICAYGLAHELTEDEWDSMIDVDLKGPWMVARRIIPVMIRQGSGVIINNSSVGGLR
                     GMGRLSHYAAAKWGLTGLTKSWAIELAPHGIRVVSTHPTGVNTPMNDGLAELEGLTPR
                     EIGERSAGNLMPVPWIEPEDVAGAVVYLASDAARFVTGAQFVIDAGLLTR"
     gene            complement(2775..3302)
                     /locus_tag="VF720_04470"
     CDS             complement(2775..3302)
                     /locus_tag="VF720_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878636.1"
                     /translation="MARIAPIEYDAASAETRRGYDEQIAAHGRVTNMKRTLGHSPVAF
                     RALMEWYPLEEAVASFLGQRRTRLFAHAISAETDCLVCSTFFRRILVDLGENPDRLEL
                     DERDRVIVDYGRQVAVDSNKVSDDLYARLAAFLAPAQIVTLTTFGALMIATNVFNNAL
                     QVDLDDYLAPYRREI"
     gene            3378..3869
                     /locus_tag="VF720_04475"
     CDS             3378..3869
                     /locus_tag="VF720_04475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878637.1"
                     /translation="MKSVGFALSLAALLMAAPVDAQSPKGRTPWKPVEGAFFALSVPD
                     IVASSRWYQDKLGLEVVLEIPVQNGTAVTILEGGGLTVELLQHATARPISSACPGVTD
                     PQMIHGFVKAGFVVKDFDGVLAELRSRGVEVAYGPFPASDIQKANVILRDNSGNLVQL
                     FGR"
     gene            3934..>6308
                     /locus_tag="VF720_04480"
     CDS             3934..>6308
                     /locus_tag="VF720_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7878638.1"
                     /translation="MSLQPGSRVGPYVIEHALGAGGMGEVYRAKDERLGRSVAIKSLP
                     GTFLGDADRLARFDREARLLASLNHPNIATIHGLEEADGLPHLVLELIDGETLQARLS
                     RGPLSVREALDIGRQVASAIEAAHDKGIIHRDLKPANVMVTSDGRAKVLDFGLARGDT
                     PKPVDSASLSASPTLAVSATAAGVILGTAPYMSPEQARGLEVDRRTDIWALGCLLFEC
                     LSGRGTFAGDTVSDVIARILERDPEWALLPPGTPPRLRELIKRCLTKDTAERPRDIGD
                     LRKELGSIATEMTSGSGIRATSDKPSLAVLYFENLSSDAESGYFCAGITEDILTDLSK
                     IKGLKVASRNAVSRYRGQTVDLARVASDLGVKAILEGSVRRAGDRVRISAQLINAADG
                     FQLWAERYDRQMADVFQVQEEIASAIAEALKVALTPAEEAAIGGDRPTDVKAYDLYLR
                     GREAYRQYTAESLREARQCFQDAIDLDPDYSLAWAGLADTFAQMHGYQVADDPVEALR
                     LGEEAARRSIALNPMLAEGHKALSLVMRYAGQIGEAEASLRRALEVNPRYTPAMINLA
                     VRLFDIADLAGAERQFRRALEIDPQETFAVSWLAFLLSLEGRYEETWQLYERVYRVAD
                     TPAYRTIVYSARVFVRLRQSDTTGAAAILPDAGADGCHPATLAILRAAVDCRAGRIEE
                     ARAAMAELGDPPGLTFYGGLLLGEVQITLGEWDRAFRTMDRPVWRSEMPTAIRLIPAL
                     HALADRAPFAPRRSDSTLVWPVEAPMMSPAVYAMFREVKIESGGAESSQPG"
BASE COUNT         1055 a         2193 c         2043 g         1017 t
ORIGIN      
        1 cgcgaggcca cgcggcactc ggtgaacctg gtctacatcc gcctgatgcg cgacatcgtg
       61 cgccactaca ccaccgagat tcccgacttc gattcggaga tgtacgagaa ccggagccat
      121 ccgctccggc gccgttacct cgagaagttt gccgatcgtg aagggaagca gttcctggcc
      181 cgtttcctcc cccgttaccg cggcaagacc agcgaggaga tcctcgatcg cgtggtggag
      241 gagatccggc cgacaccctc gcgcctggcg gtcatcttcc ggtcggtcaa gccggccggt
      301 tcggtcgatg aattccgccg gtgggtggcc cgcgtctggc cggcgggttt ggggaccgag
      361 cggggcgaat ccgcgctgcg cgatgcctac caacgctatc cgcgcacggc cttcgacatc
      421 cacgaccgcg gctacatttc ggggttgcat ccactcgagc tctggctcgt gcagtacctc
      481 tacctgaatc ccacggccgg ctggagcgat ctcgtggccg ccagcaccga agagcggcag
      541 ggagtctaca agtggctctt caactcgaag agcctggatg ggcagaacaa ccgcatccgc
      601 atcctcatcg aggaagaggc cttcaagaag gtgcacgccg cctggcgcaa gcagggctac
      661 cccttctcga gcctcgtgcc gtcttatgcc acgtcgatcg gctcctccgc cgaccggccg
      721 gcggccctgg ccgagctcat ggggatcatc gtgaacggcg gcgtccggtt tccgctgacg
      781 cgcatcgaca cgatgcacct ggccgaggga acgccctacg agatactcta cgagcaccat
      841 ccgatggaag gtaagagggt ctacccggcc gaactcacca gcgtcgtgcg aaaggccatc
      901 accgacgtgg tggaaaacgg cacggcccgc cgggtgtacg gtgccttcaa gagggccgat
      961 ggaagcgtga tcccggtcgg cggcaagacc ggcaccggcg accatcgcta cgacacgtat
     1021 gccccgggtg gccgcctcat cgaatcgcgc gtggtgaaca ggacggctac gttcgtcttc
     1081 ctgatcggcg accacttctt cggcacgctt accgcctacg tggccggccc caaggcggcc
     1141 ggctacgcct tcaccagctc cctgccggtg cagatattga aaacgctggc cccctcgctc
     1201 gagccgctga tcctgtcgga tgagccgggc ccgacgcagg ctgcggcagt ttcgtcggcg
     1261 ggcgcggatt ccctccaccg accgatcgac ccgcacgcgg aataggtttc ggggcgattg
     1321 ccccggactg gattcaactc agggtcgcgc gagcgccatg ccgcacgaaa aatcatgtca
     1381 aagggcctat gttcggataa gtatcggggt gcctgcgcca actcccccga agggccgtcg
     1441 tcgaatcacc cgaattgtcc cgccggaggc agtccgcacc ggggtgccag ggacgccgcc
     1501 actaccctga tctgacatct cggcctcccc tcgtccgcgg aagtgatgaa actttccacg
     1561 gttgccgagc cggagattgt gtggcccgcg gaggcgtgag gtcgccgcgc acccgtcctc
     1621 gccccgtggc acatcccctg caatatgtcc ctgcgtccgg gcgacgcgag tcgatcgggc
     1681 ctgaacgaga acgagacgat gttctctgca gtgaccgcca ccggtgctgt cggctgatcc
     1741 cccattaatg gatggattcc agcgctgcgg cggaacggca cagagtggag agcatcctca
     1801 tctacggcgc gaccgtcgac tcatcctcga cacgccaccc gatacgacgg tcgcgccacc
     1861 atatctcctg acagctccgc gtggtcgctc gaaagagacg gccggacctc tggctcaggt
     1921 ccggccgttt cacttttccg ccgtctcgga atccatcgcg cccttaccgc gtcaacagcc
     1981 ccgcgtcgat cacgaactgc gccccggtca cgaaccgcgc cgcgtcggag gccaggtaga
     2041 cgacggcacc tgccacgtcc tcgggctcga tccagggaac cggcatcagg tttccggcgg
     2101 agcgctcgcc gatttcgcgc ggcgtgagcc cctccagttc ggccaggccg tcgttcatcg
     2161 gcgtgttcac gccggtggga tgcgtggaga ccacgcgaat gccgtgcggc gccagctcga
     2221 tggcccacga cttggtgagg ccggtgagcc cccacttggc ggcggcgtag tggctgagtc
     2281 ggcccatgcc gcgcaggccc ccgaccgacg agttgttgat gatcacgccc gaaccctgcc
     2341 ggatcatgac ggggatgatg cggcgggcca ccatccacgg tcctttcagg tcgacatcga
     2401 tcatggaatc ccattcgtcc tcggtgagct cgtgggccag gccgtaggcg cagatgccgg
     2461 cgttgttgaa cagcacgtcg atccggccga gctcgttcac cgtcgtttcg accgcgcggg
     2521 tgatcgccgc gtcgtcgcgg acgtcaccgg cgaacgtgag gcatcgaacg ccaccggctt
     2581 cgcaggcggc ggccagcgac ttcagatcgt cttcctgccc cagcgcgtag ccgggatagg
     2641 cggtcgtgcg cgcgatgtcg tacgccgcga tgtgggcgcc ctcggccgcc agggccaacg
     2701 ccgaggcccg tccctgtcca tgcgcggcgc cggtgatgaa ggccaccttc cccttgattc
     2761 ccgtctccat gcgatcagat ctccctgcga tacggcgcca gatagtcgtc gagatcgacc
     2821 tggagcgcgt tgttgaacac gttggtggca atcatcaacg ccccgaacgt cgtgagcgtg
     2881 acgatctggg cgggtgcgag gaaggcggcg agacgggcgt acagatcatc ggaaaccttg
     2941 tttgaatcga cggccacctg gcggccgtag tccacgatga cgcgatcccg ctcatcgagt
     3001 tcgagacggt ctgggttctc gccaagatcg acaagaatcc gccgaaagaa cgtcgaacag
     3061 acaaggcaat cggtctccgc cgaaatggcg tgggcgaaca gccgggtgcg gcgctgtccg
     3121 aggaacgagg ccaccgcctc ctcgagcgga taccattcca tgagcgcgcg gaacgcgact
     3181 ggggagtgcc ccagcgtccg cttcatgttg gtcacgcggc cgtgggccgc gatctgctca
     3241 tcgtagccgc gccgcgtttc cgcactcgca gcatcgtatt cgatgggagc tatccgggcc
     3301 atgtcatcac ctccgattgc cgaccgtagg ctaccattat gtgacgctgg gcttgattac
     3361 atcacgagga ggaccccatg aagtcagtcg gtttcgcact cagcctggcc gcgctcctga
     3421 tggccgcacc ggtcgacgcg cagagcccga agggacgcac tccctggaaa ccggtggaag
     3481 gtgctttctt cgcactctcg gttcccgaca tcgtggcgag ttcccgctgg taccaggaca
     3541 agctcggact cgaggttgtg ctggaaatcc cggtccagaa cggaaccgcc gtcacgatcc
     3601 tcgaaggggg cggcctgacg gtggaactcc tccagcacgc cacggcccgc ccgatttcaa
     3661 gcgcctgtcc gggtgtcacc gatccgcaga tgatccacgg cttcgtgaag gccggattcg
     3721 tcgtgaagga cttcgacggc gtcctcgcgg agctgcggag ccgcggcgtc gaagtcgcct
     3781 acgggccgtt tccggcctcg gacatccaga aggccaacgt catcctgcgg gacaactcgg
     3841 ggaacctggt ccagctgttc gggcggtgat gaggcgcagg ccgcaccgca cgcgccacgc
     3901 cggcagtccg ggatggggta tcatcgtcgc cccatgagcc tccaacccgg cagccgcgtc
     3961 ggtccctacg tcatcgagca cgccctcggc gccggtggca tgggcgaggt gtaccgcgcg
     4021 aaggatgagc ggctgggacg gtcggtggcc atcaagtccc ttccaggcac gttcctcggt
     4081 gatgccgacc gcctggcccg cttcgaccgc gaggcgcggt tgctcgcctc gctcaatcac
     4141 ccgaacatcg ccacgatcca cggcctcgag gaggccgacg gcctccccca ccttgtcctc
     4201 gaactgatcg acggcgaaac gctccaggcc cggctgtcca ggggaccgct ttcggtacgc
     4261 gaggccctcg acatcgggcg acaggtggcg tcggccatcg aggcggcgca cgacaagggg
     4321 atcatccacc gcgacttgaa accggccaac gtgatggtca cctccgacgg ccgggccaag
     4381 gtgctcgatt tcggtctcgc gcgcggcgac acgccgaagc cggtcgactc ggcgagcctg
     4441 tcggcctcgc ccacgctcgc agtgagcgcg accgcggcgg gcgtgatcct cggcaccgct
     4501 ccctacatga gtccggaaca ggcccgcgga ctcgaagtgg atcgtcgcac cgacatctgg
     4561 gcgctcggct gcctgctgtt cgagtgcctg agcggccgcg gcacgttcgc gggcgatacc
     4621 gtttcggacg tcatcgcccg catcctcgag cgagatccgg agtgggcgct gctgccgccc
     4681 ggcacgccgc cccgactgcg cgagctcatc aagcgctgcc tcaccaagga cacggccgag
     4741 cggccgcgcg acatcggcga cctgcggaag gagctgggct cgatcgccac ggagatgacg
     4801 agcggctccg gcattcgcgc caccagcgac aagccgtcgc tggccgtcct ctatttcgag
     4861 aacctgtcga gcgatgcgga aagcggctac ttctgcgccg gcatcaccga ggacatcctt
     4921 accgacctct cgaagatcaa gggactcaag gtggcctcgc gcaacgcggt gtcccgctac
     4981 cggggccaga cggtcgacct tgctcgcgtg gcctccgacc tcggcgtgaa ggcgatcctc
     5041 gaagggagcg tccgccgggc gggcgatcgc gtgcgcatct ccgcgcagct catcaacgcc
     5101 gccgacggtt tccagctctg ggccgaacgc tacgaccggc agatggccga tgtcttccag
     5161 gtgcaggagg agatcgcctc cgccatcgcc gaagcgctga aggtggcgct gacgcccgcc
     5221 gaggaagcgg ccatcggcgg cgatcggccg accgacgtga aggcgtacga cctgtatctc
     5281 agggggcgcg aagcctaccg gcaatacacg gcggagtcgc tgcgcgaagc ccggcagtgc
     5341 ttccaggacg ccatcgacct cgatcccgac tactccctgg cgtgggccgg cctcgccgac
     5401 acgttcgccc agatgcacgg ataccaggtg gccgacgacc ccgtcgaagc actcaggctg
     5461 ggggaggagg cggcccgtcg ctccatcgcg ctgaacccga tgctggctga gggacacaag
     5521 gcgctctcgc tggtgatgcg ctacgccggg cagatcggcg aggccgaagc cagcctccgc
     5581 cgggccctcg aggtaaaccc ccgctacacc ccggccatga tcaacctggc cgtgcgactc
     5641 ttcgacatcg ccgacctggc cggcgcggag cggcagtttc gccgcgcgct cgaaatcgac
     5701 ccgcaggaga ccttcgccgt gtcatggctg gcgttcctcc tgtcgctcga agggcgctac
     5761 gaggagacgt ggcagttgta cgagcgcgtc taccgggtgg ccgacacgcc ggcctaccgc
     5821 accatcgtct acagcgcccg cgtcttcgtg cgacttcgcc agagcgacac gaccggcgcc
     5881 gccgccatcc tccccgacgc gggggccgat ggctgtcacc ccgccacact ggccatcctg
     5941 cgcgcggccg tcgattgccg ggccggccgc atcgaggagg cccgcgcggc catggccgag
     6001 ctgggtgatc ctccgggcct cacgttctac ggcggcctcc tgctggggga agtgcagatc
     6061 accctcggcg agtgggaccg cgcgttccgg acgatggacc gccccgtctg gcgaagcgag
     6121 atgcccaccg ccatccgcct gatcccagct ctgcacgcac tggccgaccg cgccccgttc
     6181 gcaccgcgcc gcagtgactc gacactcgtc tggcccgtcg aggccccgat gatgtcgccc
     6241 gccgtgtacg ccatgttccg cgaggtgaag atcgagtcgg ggggcgccga atcgagccag
     6301 ccggggat
//
LOCUS       DASVOD010000208         4368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_14399, whole genome shotgun sequence.
ACCESSION   DASVOD010000208 DASVOD010000000
VERSION     DASVOD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14399"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1867)
                     /locus_tag="VF720_04485"
     CDS             complement(272..1867)
                     /locus_tag="VF720_04485"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX7878639.1"
                     /translation="MTQYHADRKFTLAGAPRHIRVLITSFNVIVLIALLVGVVNYWDK
                     TGMTPAGVRAWYRGNEGEAVIDDAPMTFEKTFRELLDATHPHLFGQGVLLFILSHIVA
                     LTGLSERRKIAIYLLSFGAMLLDAAMPWLIRYVSPGLAPLSIVSLLFLTCMFALQVIV
                     PIREMWFGQRRTVPGAGAAVLLLVALGMTGVHGDTRAADLAESRRLWLVMGTTCEVTL
                     GTADDELARRGFDAAWRELALVDSLMSLYRPSSELCRVNREAAHAPVAVSEPTFTVID
                     AAQRMAESSDGAFDITVKPLMDLWGFYRKAGHRPDATAIDSTRALTGWQRLVLDRELH
                     TVRFTTPGMALDLGGIAKGYALDRAMAAVMRCGIRDALIDLGGNIIARGSAGHDAPGW
                     PVALRDPACPDSVTSLAWLVNEAISSSGGYEKSVVLDGVTYGHILDVRRGEPVRGVAG
                     TTVVAPGAMQGDALSTTLAVLGSEGLAWLAARYPGVEAVVTLQDGGTGKSFYSAGSQL
                     APHDVPDESDTNHPSAPEAARRR"
     gene            complement(1864..2430)
                     /locus_tag="VF720_04490"
     CDS             complement(1864..2430)
                     /locus_tag="VF720_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEX7878640.1"
                     /translation="MRRLRVVFVAVLVAACPVRAEEPVPDMPVTVYQSAGQAMLETFP
                     TADRFDAETKILAADQRAALEKALGGRVADDTVHVHRAWRDSSLVGYGVVSEEIGKYR
                     PITFLVGTTPDLVVARVTVLVYRESHGADVRRTRFLNQYRGKEWDDPIRLNKDIINIS
                     GATLSVRSLNFGVRKVLGILGAFYGERP"
     gene            complement(2430..3620)
                     /locus_tag="VF720_04495"
     CDS             complement(2430..3620)
                     /locus_tag="VF720_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7878641.1"
                     /translation="MVRRAMLALLFLATTPFLASAADDPAVDPAATDKPRMPGSVYDK
                     PFLYRGGDGNFMVGGYLDLELHANESTSTFTAHRFVPFIYGQISDRVSVASEIEFEYG
                     GAVAGDEETDGEIKVEFAHMDFRMTDAFQFRGGILLSPLGRFNLVHDSAVNDLTERPL
                     VDQQIIPSTLSEAGLGFFGTIETGESSLLSYEAYLVNGFNQGILEENGEIRNREGRGS
                     AKTDNNNARSFVGRLGFSPMLGLDLGASAHTGRYTDRTSSSENLSIVAFDARFNRGPL
                     ELLGEYALSGAGLPDGITLEGADEVNQNGYYVQANWHFLSGTIKSMPASIMTAVVRFD
                     EVDFDTDITGDRNRRFAVGLNFRPTEDTAFKADVTRGWRADRGVEGDGLPQDSAHFSV
                     ATYF"
     gene            complement(3773..>4368)
                     /locus_tag="VF720_04500"
     CDS             complement(3773..>4368)
                     /locus_tag="VF720_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7878642.1"
                     /translation="ELYRTMRRVVRGNVPASRLAVLVARRHATFPEHAMPMRFVENHD
                     EYRAGRRLGASARALAVFAALSGGCFLVYNGQEVGARLRPNLFDRDPINWMSPDAAIH
                     RAWWRGLIEMRRGLHDFGAPEAIETGIPVLAAYRRRGAKGERIVAFNGSARPVDWRPE
                     GVVGESIVVQPGSMTWPPSAGRSFRIPAHGVIVYLSY"
BASE COUNT          766 a         1438 c         1471 g          693 t
ORIGIN      
        1 gtgagtcccg ctcggaaaaa ttcaaggccc cgctcacctt tcggtgaacg gggcctctcc
       61 atcacatcat cgcttacatg atggaagccg ctcggggacg atgaaggcct tcgccgtccc
      121 ctcgcgggcc gcgactgaag cagtgcctat cctccagtcg ccggaaacac cgtccgggtt
      181 accagcccaa ccgccgcgtt tccctgtcca gaatataggt cgagccatcc gactgagcca
      241 cactcatctg gtaaaaagag ggtaaaagtt gttatcggcg ccgggccgct tcgggtgccg
      301 atggatgatt cgtgtccgat tcgtccggca cgtcatgtgg tgccaattgc gatccggcgc
      361 tataaaaaga ctttccagtc ccgccatcct gaagcgtcac cacggcttcg acgcccgggt
      421 agcgtgcggc gagccaggca agtccctcgg atccgaggac ggcgagggtg gtcgagagcg
      481 catcgccctg catggccccg ggagccacga ccgtagtgcc ggcgacgccg cgcacgggct
      541 ccccgcgccg gacatcgagg atgtggccgt aggtgacgcc gtcgaggacg acggatttct
      601 cgtacccccc cgaggaggag atggcctcgt tcacgagcca cgcaagtgac gtgaccgagt
      661 cagggcacgc gggatcacgc agcgccaccg gccagcccgg cgcgtcgtgg ccggcgcttc
      721 ccctggcgat gatgttgccg ccgagatcga tcaacgcatc acgaatgccg caacgcatga
      781 ccgccgccat cgcgcgatcg agcgcgtaac ccttggcgat gccgcccagg tcgagcgcca
      841 tccccggcgt cgtgaatcgg acggtgtgca attccctgtc gaggaccagc cgttgccagc
      901 cggtgagcgc gcgtgtggaa tcgatggccg tcgcgtccgg acgatgaccg gctttgcgat
      961 agaaccccca caggtccatg agcggcttca cggtgatgtc gaacgcgccg tccgacgatt
     1021 cggccatccg ctgcgcggcg tcgatcaccg tgaacgtcgg ctcgctgacc gcgaccggcg
     1081 cgtgcgccgc ctcccggttc acccggcaga gctcgctcga tggccggtaa agactcatca
     1141 gcgaatcgac gagcgcgagc tcccgccacg cggcatcgaa gccgcggcga gccagctcat
     1201 cgtcggccgt accgagcgtc acctcgcagg tcgttcccat gacgagccag aggcggcgcg
     1261 attccgcgag gtcggcggcg cgggtatcgc cgtgcactcc ggtcatcccg agcgcgacga
     1321 ggagcagcac ggccgcaccg gctcccggaa cggtccggcg ttgcccgaac cacatctccc
     1381 ggatcggcac gatgacctgc agcgcgaaca tgcacgtgag gaaaaggagc gacacgatcg
     1441 agaggggcgc cagcccgggc gatacgtacc ggatgagcca cggcatggcg gcgtccagga
     1501 gcatcgcccc gaacgacaga aggtagatgg cgatcttcct ccgttcggac aggccggtga
     1561 gcgccacgat gtgggacagg atgaagagga gcacgccctg tccgaacagg tggggatgcg
     1621 tggcgtcgag cagctcgcgg aacgtcttct cgaacgtcat cggcgcgtcg tcgatgaccg
     1681 cctccccctc gttgccgcga taccacgccc ggacgccggc cggcgtcatg ccggtcttgt
     1741 cccagtagtt caccacgccc acgaggaggg cgataaggac gatcacgttg aagctggtga
     1801 tgaggacccg gatgtgacgg ggcgccccgg ccagcgtgaa tttccggtcg gcgtggtact
     1861 gggtcacggc cgttccccgt agaacgcgcc aaggatcccc agcaccttcc tgaccccgaa
     1921 gttgagggaa cggaccgaga gcgtggcgcc gctgatgttg atgatgtcct tgttcaggcg
     1981 gatgggatcg tcccattcct tgccgcggta ctggttcagg aaacgcgtgc gccgcacgtc
     2041 cgcgccgtgg gactcccggt acaccagcac cgtgacgcgc gccacgacga gatccggcgt
     2101 cgtgcccacg aggaaggtga tcggccggta cttgccgatc tcctcgctca ccacgccgta
     2161 gccgacgagg ctcgaatcac gccaggcccg gtgcacgtgg acggtgtcgt cggcaacgcg
     2221 gccgccgagg gccttctcga gtgccgcgcg ctgatcggcg gcgagaatct tcgtctcggc
     2281 atcgaagcga tcggccgtcg gaaacgtctc gagcatggcc tgcccggcgg actggtacac
     2341 cgtcaccggc atgtccggca cgggctcctc ggcccggacc ggacatgcgg caacgagcac
     2401 cgcgacgaag acgacgcgga ggcgtctcat cagaagtagg tggccaccga gaagtgggcc
     2461 gagtcctggg gcaggccgtc gccctcgaca ccccggtcgg cgcgccagcc tcgggtcacg
     2521 tcggccttga aggcggtgtc ctcggtgggg cggaagttga ggcccaccgc gaagcgacgg
     2581 ttgcggtcgc cggtgatgtc ggtgtcgaag tcgacctcgt cgaagcggac gacggcggtc
     2641 atgatcgagg ccggcatcga cttgatcgtg cccgacagga agtgccagtt ggcctgcacg
     2701 tagtagccgt tctggttcac ctcgtcggct ccctcgagcg tgatgccgtc gggcaggccg
     2761 gcccccgaga gggcgtactc gccgaggagt tcgagcgggc cgcggttgaa gcgggcgtcg
     2821 aacgccacga tcgagaggtt ctcggacgac gaggtgcggt cggtgtagcg gccggtgtgg
     2881 gccgaggcgc cgaggtcgag gccgagcatc gggctgaagc cgagccgccc gacgaacgag
     2941 cgcgcgttgt tgttgtcggt cttggcgctg ccgcgccctt cacggttccg gatctcgccg
     3001 ttctcctcga ggatcccctg gttgaagccg ttgaccaggt aggcctcgta cgacaggagc
     3061 gatgactcgc ccgtctcgat cgtgccgaag aagccgagcc ccgcctcgga gagcgtgctc
     3121 gggatgatct gctggtcgac cagcggccgc tcggtcaggt cgttgaccgc cgaatcgtgc
     3181 accaggttga agcggccgag cggcgagaga aggataccgc cccggaactg gaacgcgtcg
     3241 gtcatccgga agtccatatg ggcgaactcc accttgatct cgccgtcggt ctcctcgtcg
     3301 cccgccaccg cgccgccgta ttcgaactcg atttcgctgg ccaccgacac gcggtcggag
     3361 atctggccgt agatgaaggg gacgaagcgg tgcgccgtga aggtgctggt cgactcgttg
     3421 gcgtgcagct cgaggtcgag gtagcccccc accatgaagt tgccgtcgcc gccccgatac
     3481 aggaacggct tgtcgtacac gctccccggc atccggggct tgtcggtggc cgccggatcg
     3541 accgcggggt cgtcggcggc cgaggcgagg aacggcgtgg tggcgagaaa gagaagcgcg
     3601 agcattgccc tgcggaccat ggatctgtct cctggattgt ggaataccta ccaactgtca
     3661 gctatcccta acggggaggc gggagactaa ttgccatcgg agcccccggt caatgtcaaa
     3721 tatgtctaac atgcgtttgc cacgaccaac gcaggatcta atttaaatag attcaataac
     3781 ttagatacac aatgaccccg tgcgcgggaa tgcggaagct cctgccggca ctcggcggcc
     3841 aggtcatcga gcccggttgg accacgatcg actcgccgac caccccttcc ggccgccagt
     3901 cgaccggccg ggcggagccg ttgaaggcca cgatccgctc ccctttcgca ccacgccttc
     3961 ggtacgcggc cagcaccggg atcccggtct cgatcgcctc gggtgccccg aagtcgtgaa
     4021 gtccgcggcg catctcgatg aggcctcgcc accaggcccg atgaatggcc gcgtccgggg
     4081 acatccagtt gatcgggtcg cgatcgaaga ggttcgggcg aagacgcgcg ccaacttcct
     4141 ggccgttgta gacgaggaag cagccaccgg acagggcggc gaacactgcg agagcccgcg
     4201 ccgacgcgcc gagccggcgc cccgcgcgat actcgtcgtg gttctcgacg aagcgcatcg
     4261 gcatggcgtg ttccggaaac gtggcgtggc gccgagcgac gagtacagcc aggcggctcg
     4321 cgggcacgtt gccgcggacg acacgccgca tcgttcgata cagctccc
//
LOCUS       DASVOD010000209         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_274609, whole genome shotgun sequence.
ACCESSION   DASVOD010000209 DASVOD010000000
VERSION     DASVOD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274609"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..959)
                     /locus_tag="VF720_04505"
     CDS             complement(207..959)
                     /locus_tag="VF720_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878643.1"
                     /translation="MVALRTHRNSLLAALVVAFLPTLAAAQTCADPTRCADTPVLITF
                     ESLAAGTSVEGPGAVFPPLTIQTAASALYPSCPVGSAKVIEELNPFPYGSYSTGTSGI
                     NACLTGIHGFADSADCVLDYQFTFAPGFTVSCFSVLMLDYADYFPYGGSSHSVLMEAF
                     DASNVVVDTFTLTEFGGTQPGSGDACDAQPGDPGRTTMFVTAPGIKKVRIRFTEAPDP
                     NVGFDDLSFCLQEPPTPVLPSTWSGVKSRLGD"
     gene            1092..2138
                     /locus_tag="VF720_04510"
     CDS             1092..2138
                     /locus_tag="VF720_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX7878644.1"
                     /translation="MKLTPTDNPPNRWTGLRLDYDEVPPPPADLVIFEERARTVLTAN
                     DSPDISFKWSLNPYRGCFHGCAYCYARPSHGYWDFGAGTDFERKIVVKVNAPERLREA
                     FMKPSWEGEMVAFSGNTDCYQPLEIQYEITKRCLEVCLEFRNPVGIITKGSIIRRDVD
                     LLARLAREAKASVYLSIPFADDEMARALEPWAPSPKARFDTLRILSDAGIPTGVSVSP
                     IIPGMNDSQVAEVLERSAEAGATKAFHILLRLPNEVKPVFTERLTAAYPDRVDKVMNG
                     IKAARGGRLNDPRFGSRMQGSGPRWEAVKFLFESTCKRLGLNDRRRTEFDEPGSTFRR
                     PGDQLGLFDAAAGA"
     gene            complement(2102..>2293)
                     /locus_tag="VF720_04515"
     CDS             complement(2102..>2293)
                     /locus_tag="VF720_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878645.1"
                     /translation="GTPHLTTSGATLFGQQALFAVVATLILCIVAVAVYVAWERPFLS
                     LKRFVPRPQAPAAASNNPS"
BASE COUNT          434 a          716 c          788 g          355 t
ORIGIN      
        1 gcagctcacc gtcacctaca cgccgccttc ggttccggtg gccgaatcga cctggggacg
       61 gatcaaggcg ctttcgcagt aggcacgcgc ccaaggccag cgttccctga cgtcgacact
      121 ccagaaaacg aaaacgaggc cgcccggttc atctaccggg cggcctcgtc atttgtgctc
      181 tcgaatgtca gctgtgcgtt tgaatgtcag tcgcccagac gcgatttcac gccgctccag
      241 gtgctcggca ggaccggcgt cggcggctct tgcagacaga acgagaggtc gtcgaagccg
      301 acgttcgggt cgggcgcctc ggtgaagcgg atccggacct tcttgatgcc cggggcggtc
      361 acgaacatcg tggtgcggcc ggggtcgccg ggctgcgcgt cgcacgcatc gccggaaccg
      421 ggctgcgttc cgccgaactc ggtcagcgtg aacgtatcga cgaccacgtt cgatgcgtcg
      481 aaggcttcca tgaggaccga atgcgatgag ccgccgtacg ggaagtaatc ggcgtagtcg
      541 agcatcagga ccgagaagca cgacaccgtg aagccgggcg cgaaggtgaa ctggtaatcg
      601 agcacgcagt cggcgctgtc ggcgaagccg tggatgccgg tcaggcatgc gttgatgccg
      661 ctggtcccgg tgctgtagga gccatacggg aacgggttca actcctcgat gaccttcgca
      721 cttccgaccg ggcacgacgg gtaaagcgcc gacgcggccg tctggatcgt gagcggggga
      781 aaaacagcgc ccggaccctc gacactggtg cccgcagcaa ggctctcgaa cgtgatcagg
      841 accggcgtgt cggcgcaacg cgtcgggtcg gcgcaggtct gcgccgcggc gagggtggga
      901 aggaatgcca cgacgagcgc ggccagcagg gaattgcggt gcgtacgcaa ggcgaccatg
      961 ggacctccga ggggtgcgga accgtgtggt gcgggcggcc ggctacatca tataaggggt
     1021 cggggcaggc gatgacaaac gccatgaaga aaagtttaaa ataccagaca aattaatagt
     1081 ataatcgtca ggtgaaactg acgcccaccg acaacccccc gaatcgctgg accggactcc
     1141 ggctcgacta cgacgaggtg ccgccaccgc cggccgacct cgtcattttc gaggagcggg
     1201 ccaggacggt gctcacggcc aacgattcgc cggacatcag cttcaagtgg agcctgaacc
     1261 cgtatcgcgg ctgcttccac ggctgcgcct actgttacgc gcgtccctcg cacggctact
     1321 gggatttcgg cgcgggcacc gacttcgagc ggaagatcgt ggtcaaggtc aacgcgccgg
     1381 aacggttgcg cgaggcgttc atgaaaccgt cctgggaggg ggagatggtg gccttcagcg
     1441 gcaacaccga ctgctaccag ccgctggaaa tccagtacga gatcacgaag cgctgcctcg
     1501 aggtgtgcct cgagttccgg aacccggtcg gcatcatcac caaggggtcg atcatccggc
     1561 gtgatgtcga ccttctcgcc cggctggcgc gggaggcgaa ggcttcggtc tatctgagca
     1621 ttcccttcgc ggacgacgag atggcgcgcg cactggaacc ctgggcgccg tcgcccaagg
     1681 cccgcttcga cacgctgcgc atcctctccg acgcgggcat tccaaccggc gtgtcggtgt
     1741 cacccatcat cccggggatg aacgacagcc aggtagccga ggtgctggag cgatcggcgg
     1801 aggcgggggc cacgaaagcg ttccacatcc tgctcaggct gccgaacgaa gtgaagccgg
     1861 tcttcacgga acggctcacg gccgcgtatc ccgaccgggt cgacaaggtc atgaacggca
     1921 tcaaggcggc ccgcggcggg cggctcaacg atccccgctt cggttcgcgc atgcagggga
     1981 gcgggccgcg ttgggaggcg gtgaagttcc tgttcgaatc cacctgcaag cggctcggcc
     2041 tgaacgaccg ccggcgcacc gagttcgacg agcccggcag caccttccgc cggccggggg
     2101 atcagctcgg attgttcgac gcggcggccg gcgcctgagg gcgcggcacg aaccgcttga
     2161 gtgacaggaa cggccgttcc caggcgacat acacggccac cgccacgatg cagaggatga
     2221 gcgtggccac cacggcgaag agcgcctgct ggccgaagag cgtggcaccg gatgtcgtga
     2281 ggtgcggtgt gcc
//
LOCUS       DASVOD010000210         8693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_743669, whole genome shotgun sequence.
ACCESSION   DASVOD010000210 DASVOD010000000
VERSION     DASVOD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8693
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_743669"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2380)
                     /locus_tag="VF720_04520"
     CDS             complement(<1..2380)
                     /locus_tag="VF720_04520"
                     /inference="COORDINATES: protein motif:HMM:NF025152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 family protein"
                     /protein_id="HEX7878646.1"
                     /translation="MENWKAAHIEIARRIQDGRRHLTREIVAAGLLRFLAVALIGFVV
                     GTALHLVLRFGTAPAFGLSAAALGAAGYALWRMLLKPYRSRPDEERFVEWLDARHDRD
                     KNLIVSAWQLGKESPGSVRFAPDLVDAVVQRGARAAGAIELTGWRDRENDRRWWIGLG
                     SALAAVALLVVVAGPQRFGGAAMKLVNPRMAALAPVSLLVQPGDLTVERGTDVDLDIK
                     VTGTTEPPVLKVRETGGIWMTRRLAQPTELPAALGGPIGYRSSLARVERDQEYQVVVH
                     KTESPVFRIAVNEAPRVAGFEITYYYPDYTGRDPETVTSGSGDLAALQGTRVLLKVLA
                     NRPMGSALMRQGPAGAPELTEGTPLDAVDAPNWETRLTLAGEPKEYAIVLRDAGGEER
                     FTSPRFRIEAAPDRPPVIRLVSPEKDMPIPEEMTLGLSAEAVDDYGLSRLDLVYEVKD
                     GAQGRVRLKDFAAGTSELHGDFAWDLVPLNLTPGSDVSYFLEIWDNDRVTGPKSARSD
                     VRHLRFPTMEEIYAEVQDEHEQQIDDLSSTLQQGKELQEQLEKLARESKRGDEMSWDK
                     KKEMENLLEKQAELEKQLEETARQLEETLKKAQEETFISPELLQKMQEISDLMNSIKN
                     EKLKESFEKLNEALKSMDQEAINKALEDFKVDQEEMVKGLDKTIEMLKEIRKEEMMED
                     SVRKAEELAREQEQLARDIQKEAEQDKNGKDPNGKDPGAEQKENQDLADRQAEAQKKA
                     EELARQMEELSKLAENEKKLQEELNQMQESQQKKDMKENMEQSEQSLRQQEKE"
     gene            complement(2392..3045)
                     /locus_tag="VF720_04525"
     CDS             complement(2392..3045)
                     /locus_tag="VF720_04525"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX7878647.1"
                     /translation="MVASILAIGAGVVPASAGVGFRAARLKYGGGGDWYSNPSSLPNL
                     MSNVSQRTGIPVEGEGEEAQVGLDDPALYLYPLVYMNGHGEVKFTESELASLRKYLEG
                     GGFLWADDNYGMDASFRREMRRLYPESPLVELPFDHPIYHSFYSLPQGLPKIHEHDGK
                     PAQGFGLFRDGRLVAYYTYETDIGDGIEDAEVHNDTAAVRELAMRMAVNVVVYVMNH"
     gene            complement(3475..4482)
                     /gene="secF"
                     /locus_tag="VF720_04530"
     CDS             complement(3475..4482)
                     /gene="secF"
                     /locus_tag="VF720_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX7878648.1"
                     /translation="MLQILHGTKIDFMGKRKMAYAFSALVLLAGFASLIMKGGPRPGI
                     DFAGGTLLEVRMSQAIPADQLRHAADQAGMAGAEIQTYPGTNDALFRVHEAHDQAATP
                     GSSPSAKLQQALAASHPGVTVEVLRQEVVGPKVGRELTGKAFMAILFSVIAIMIYVAF
                     RFHGWVFGVGAALALFHDILVTLGIFSILNKEIGLTVLAAFLTVAGYSVNDTIVVFDR
                     VRENLEKNRKTPLSDLMNLSINQTLSRTILTVATVMMTAAALFFLGGEVLRDFSLALL
                     IGVGFGTYSSVYVASALALDLDNWRRRKLAAKGELAKPVPTPPSTGRKAGKATQAVAS
                     R"
     gene            complement(4493..6055)
                     /gene="secD"
                     /locus_tag="VF720_04535"
     CDS             complement(4493..6055)
                     /gene="secD"
                     /locus_tag="VF720_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX7878649.1"
                     /translation="MSKTAWRGLFIALVTIAFGIVMYPTYQYYSMSKAAQAAMPKDKH
                     DKLRAQTLNLGLDLQGGAHLVYEVKTEGLPPDQRLDAVDRAIETIRNRIDQIGVGEHV
                     VQKEGTSRVLVQLPGVLDTERAKEIVGRTARLEFKLVKNDSEIQSLLGRLDSFLAARS
                     PADSSMADSTDAGRPFTSLIRTAGGGAVLFEVDAIPAVDAMIARARSVIPGNVSLAWS
                     RETSNLSGKPGRFLYFLENKVDLTGDNLQNAEYAVGVDPKSPGGSGVRLFMTKEGGAI
                     FYRLTRANVNRQLAIVMDNSVYSAPRINDAIKGGEAVITGIDQDQEARDLAVVLRAGA
                     LPADMSIVEERTVGPSLGSDSIKQGVTAAWVGAALVIVFMLVYYQGSGLIAVIAMLLN
                     VLYLVAVMVLIGATLTLPGLAGIILTIGMAVDANILVFERIREELTGGKTVRAAVDAG
                     FKRAFLTIFDAHVTTLLTAVALYVFAAGPVKGFGMTLGIGIVLNLFTAVFCSRVMFDA
                     FTERGTAKRLSI"
     gene            complement(6130..7257)
                     /locus_tag="VF720_04540"
     CDS             complement(6130..7257)
                     /locus_tag="VF720_04540"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HEX7878650.1"
                     /translation="MILILALVGLVGFLVMPAGRRLLFGARVMTATADPQVPATELAR
                     LFGESLKAALAPVGVDTTLVRVESLAAGEKGSLHQRWEVPLPRGLSADSASTLLAAAA
                     VPFHGKPQPAPEGGNSGFATRLRADVQVEAVFLVPDARTDGRPRLALLVHDFVQGKES
                     REIIDFAREITVAIPPGTTGARDLADGCRRVGIEVLVDLPMEDLNYPTNDPGPKGILV
                     DLTEREIMKRVGEAYKLLGGAEGVHTLGGNLAVEDRSVMTAIIGEVKKRRGYFLDSTR
                     STFSTVEEVAAAAGVPSRKVRSPGALDYPTATAAQVEAKLAELAETARSRGVAVGILR
                     PYPGTVEALRKMLPAWAASGLEVVPVSEVVAIPGKGANPSR"
     gene            complement(7314..7940)
                     /locus_tag="VF720_04545"
     CDS             complement(7314..7940)
                     /locus_tag="VF720_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878651.1"
                     /translation="MRSFRTARLTTAGLLFGALALTGTYGCTEIDDGPQDPGRYEPQT
                     SEDNVINNLQVAYRQRQIGKYAELLASDFQFYFDPKTRGQLGIEFWSRTTDSLQTEVL
                     FNSSDVTSIKIELTWPKRSASGAGYLTPREGWTKLFLTDVFLDVDVQTPGQPVTTLRV
                     EDQTQRFYFRKGRTYPPSGPADTLMYLVEWRDEGDPDAPGSVSGLKAN"
     gene            complement(8069..>8693)
                     /locus_tag="VF720_04550"
     CDS             complement(8069..>8693)
                     /locus_tag="VF720_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878652.1"
                     /translation="MRDNARRHAAMFAALATLTLAGCGYFAPRDPDEPGDPPDRHAPT
                     APESVLFNFQQALQEKVLGAPQLAETIDPDFILDLDERDSDRLNGLQVIFKDEFETAN
                     RLFLSLRIGANDLSVLFDTTSTPLVPETADSAFYNRLPYKLLVFPQGQSVAIDSIKGE
                     VNLRFKRRTNWAIVAWEDFDVDQLRTETYGYYMGFYAGVSAPGDILK"
BASE COUNT         1429 a         2796 c         2894 g         1574 t
ORIGIN      
        1 tttccttttc ctgctgacgg agcgactgct ccgactgctc catgttttcc ttcatgtcct
       61 tcttctgctg ggactcttgc atctggttga gctcttcctg gagcttcttc tcgttttcgg
      121 ccagcttgga gagctcttcc atctgcctgg ccagctcctc ggccttcttc tgcgcctcgg
      181 cctggcgatc ggccagatcc tggttttcct tctgctcggc gcccggatcc ttgccgttcg
      241 gatcctttcc gttcttgtcc tgctcggctt ccttctggat gtcgcgggcc agctgctcct
      301 gctcgcgggc cagctcctcg gccttgcgga ccgagtcttc catcatctct tccttgcgga
      361 tctccttgag catctcgatg gtcttgtcga gccccttgac catctcttcc tgatccacct
      421 tgaagtcttc cagggccttg ttgatggcct cctggtccat gctcttcagg gcctcgttca
      481 gcttctcgaa cgactccttg agtttctcgt tcttgatgga gttcatgagg tcggagattt
      541 cctgcatctt ctggaggagc tcgggggaga tgaaggtttc ctcctgggcc ttcttgagcg
      601 tttcctcgag ctggcgggcg gtctcctcga gctgcttctc gagctcggcc tgcttctcga
      661 gcaggttctc catctccttc ttcttgtccc acgacatctc atcgccgcgc ttcgactcgc
      721 gggccagctt ttcgagctgc tcctgcagtt ccttcccctg ctgcagcgtg gaggagaggt
      781 cgtcgatctg ctgctcgtgc tcgtcctgca cctcggcgta gatctcttcc atcgtcggga
      841 aacggaggtg ccggacgtcg ctccgggccg acttgggccc cgtcacgcga tcgttgtccc
      901 agatttccag gaagtagctc acgtcgctgc cgggagtcag gttcagcggg acgaggtccc
      961 aggcaaagtc gccgtgaagt tcgctggtgc cggccgcaaa gtccttgagt cgcacccgcc
     1021 cctgggcgcc gtccttcacc tcgtacacga ggtcgaggcg tgacagcccg tagtcgtcca
     1081 ccgcctcggc ggagaggccg agcgtcatct cctcgggaat gggcatgtcc ttctcgggcg
     1141 acacgaggcg gatgaccggc gggcggtcgg gggccgcctc gatccggaag cggggcgatg
     1201 tgaagcgttc ttcgccaccc gcgtcgcgca gcacgatggc gtattccttc ggctcgccgg
     1261 ccagcgtgag gcgcgtttcc caattcggtg catcgacggc atcgagggga gtgccctcgg
     1321 tgagctccgg cgcgccggcc gggccctgcc gcatgagggc cgagcccatc gggcggttgg
     1381 ccagcacctt gagcaggacg cgtgttccct ggagcgcggc caggtcgccg ctgccgctgg
     1441 tgacggtttc gggatcgcgg ccggtgtagt cgggatagta gtaggtgatc tcgaagccgg
     1501 ccacgcgcgg cgcctcgttc acggcgatcc ggaacaccgg cgattcggtc ttgtgcacca
     1561 ccacctggta ctcctggtcg cgctcgaccc gggccagcga cgaccggtag ccgatggggc
     1621 cgcccagtgc ggccggcaat tcggtcggct gcgcgagacg gcgcgtcatc cagatgccgc
     1681 cggtctcgcg caccttgagg acggggggtt cggtggtgcc ggtcaccttg atgtcgaggt
     1741 caacgtcggt gccccgctcg accgtcaggt cgccgggctg cacgagcagg ctcaccgggg
     1801 ccagcgcggc catgcgcggg ttcacgagct tcatggccgc gccgccgaag cgctgcgggc
     1861 cggccaccac gaccagcaac gccacggcgg ccagcgccga tcccaggccg atccaccagc
     1921 ggcggtcgtt ctcgcggtcg cgccagccgg tgagttcgat ggcaccggcg gcgcgtgcac
     1981 cgcgctgcac cacggcatcg accaggtcgg gagcgaaacg gacgctcccc ggcgattcct
     2041 tgccgagctg ccaggcgctc acgatgaggt tcttgtcccg gtcgtgacgg gcatcgagcc
     2101 actccacgaa gcgctcctcg tcggggcgcg agcgatacgg cttgagaagc atccgccaca
     2161 gcgcgtagcc ggcggcgcca agcgccgcgg ccgacaggcc aaaggcaggc gccgtgccga
     2221 agcggaggac gaggtgcagg gcggtgccca cgacgaagcc gatgagcgcc accgccagga
     2281 agcggaggag cccggccgca acgatttccc gggtcaggtg ccgccggccg tcctggattc
     2341 ggcgggcgat ctcgatgtgg gccgctttcc agttttccat gtccgacctc gtcagtgatt
     2401 catcacgtaa acgacgacgt tgacggccat cctcatggcc agttcccgca ccgccgccgt
     2461 gtcgttgtgc acttcggcat cctcgatgcc gtctccgatg tccgtctcgt acgtgtagta
     2521 cgccaccagc cggccgtccc ggaagagccc gaaaccctgg gccggcttgc cgtcgtgttc
     2581 atggatcttg ggcagtccct gcggcaggga gtagaacgaa tgatagatgg gatggtcgaa
     2641 cggcaactcc acgaggggtg attcgggata gagccgccgc atttccctcc ggaaggaagc
     2701 gtccatgccg taattgtcgt cggcccacag gaacccccca ccttccaggt acttcctgag
     2761 gctggccagc tccgactcgg tgaacttcac ctcaccgtgc ccgttcatgt agacgagcgg
     2821 gtagagataa agcgccggat cgtcgagccc cacctgcgcc tcctccccct caccctccac
     2881 gggaatgccg gtccgctggc tgacgttcga catcaagttc ggcagggacg acggattcga
     2941 gtaccagtcc ccgccacccc cgtacttgag tcgggccgcc cggaaaccga cgccggccga
     3001 ggccggaacg acacccgcgc cgatggcaag gatcgaggcg accaatgccg ccgccgcaac
     3061 gcgacagacg gcaccgcgga tcgatctgat ggagccgatg gccggcggag gggccgggcg
     3121 gattcggggg ttcgggggtg acatcgtcgt tgcggactcc aggttgacgc tgaggtgagg
     3181 cgtctgaaca cacccctccg gcttcggttc caacacacga cggcacaaac gtttacaacg
     3241 acagcacccg tgcggaacgg atcgttccat cattatatgg gcgagttggt cagggcaaca
     3301 accaaatgag ggggtggggg gacgacgtca gtcctgcgta aacctctgtg ggaatatcaa
     3361 ttggggcgca taagcaaagc cgcgcggtcc ttgaggatcg cgcggccgat tcgaacgatg
     3421 tcgaaaagac aagaatggat gaactggctt tcgccagttc acctgcttac ctgcttacct
     3481 gctggctacc gcctgggtcg ccttcccggc cttccggccg gtggacggag gggtcgggac
     3541 gggcttggcg agctcaccct tggcggccag cttcctgcgg cgccagttgt cgaggtcgag
     3601 cgccagggcg ctggccacgt agaccgacga gtaggtgccg aagccgacgc cgatgaggag
     3661 cgccagcgag aagtcgcgga gcacctcgcc gcccaggaag aagagcgcgg ccgccgtcat
     3721 catgacggtg gcgacggtca ggatcgtgcg cgacagcgtc tggttgatgg agaggttcat
     3781 caggtcgctg aggggcgtct tgcggttctt ctcgaggttt tcacgaacgc ggtcgaagac
     3841 cacgatcgtg tcgttgaccg aatagccggc caccgtgagg aaggcggcca gcaccgtaag
     3901 gccgatttcc ttgttcagga tcgagaagat gccgagcgtg accaggatgt cgtggaagag
     3961 cgccagggcg gccccgacgc cgaacaccca gccgtgaaac cggaacgcca cgtagatcat
     4021 gatggcgatg acggagaaga gaatggccat aaaggccttg ccggtcagct cgcgtcccac
     4081 cttcgggccg acgacttcct ggcgcagcac ctcgacggtg acgccggggt gcgaggcggc
     4141 cagcgcctgc tgcagcttgg ccgatgggct cgagccgggc gtggccgcct ggtcatgcgc
     4201 ctcgtgcacc cggaagagcg cgtcgttggt gccggggtac gtctggattt cggcgccggc
     4261 catgccggcc tggtcggcgg catggcgcag ctggtcggcc gggatggcct ggctcatccg
     4321 gacttcgagc agggtaccgc cggcgaagtc gatgccgggg cgggggccgc ccttcatgat
     4381 cagcgaggca aagcccgcca ggagaacgag cgcggaaaag gcataggcca ttttccgctt
     4441 ccccatgaag tcgatcttgg ttccgtgaag gatctggagc atgaaatgac tcctaaatgc
     4501 tcaggcgctt ggcggtgccg cgctcggtga aggcgtcgaa catgacgcgc gaacagaaga
     4561 cggcggtgaa caggttcagc acgatgccga ttcccagcgt catgccgaat cccttgaccg
     4621 gacccgcggc gaatacgtac agcgccacgg ccgtgagcag cgtggtcacg tgtgcgtcga
     4681 aaatcgtcag gaaggcgcgc ttgaatccgg catcgaccgc ggcccgcacg gttttcccgc
     4741 cggtcagctc ttcccggatg cgctcgaaga cgaggatgtt ggcgtccacg gccatgccga
     4801 tggtgaggat gatgccggcc aggccgggga gcgtgagcgt ggcgccgatg agcaccatga
     4861 ccgccacgag gtacaggacg ttgaggagca tggcgatgac cgcgatgagt cccgaaccct
     4921 ggtaatacac gagcatgaag acgatgacga gtgcggcgcc cacccacgcg gcggtgacgc
     4981 cctgcttgat cgagtcgctg ccgaggctgg gcccgacggt gcgctcctcg acgatcgaca
     5041 tgtcggccgg cagggcgccg gcgcggagga ccacggccag gtcgcgggct tcctggtcct
     5101 ggtcgatgcc ggtgatgacg gcctcgccac ccttgatggc gtcgttgatc cgcggggccg
     5161 aatagaccga gttgtccatg acgatggcca gctggcggtt cacgttggcg cgcgtcaggc
     5221 ggtagaagat ggcgccgcct tccttggtca tgaagagccg gacgcccgag cctccggggc
     5281 tcttgggatc gacgccgacg gcgtactcgg cgttctggag gttgtcgccc gtcaggtcga
     5341 ccttgttctc gaggaagtag aggaagcgac ccggcttgcc ggagaggttg gaggtttcgc
     5401 ggctccaggc cagcgagacg ttgccgggga tgacgctcct ggccctcgcg atcatcgcgt
     5461 cgaccgcggg aatggcgtcg acttcgaaca ggaccgcgcc gccgccggcc gtgcggatca
     5521 ggctggtgaa cgggcgtccg gcgtcggtgg aatcggccat cgacgaatcg gccggcgaac
     5581 gggccgccag gaagctgtcg agccgcccca ggagcgactg gatttcgctg tcgttcttga
     5641 ccagcttgaa ctcgagacgg gccgtgcgtc cgacgatttc cttggcgcgt tcggtatcga
     5701 ggacgccggg gagctgcacc agcacgcggc tggtcccttc cttctgcacc acgtgctcgc
     5761 ccacgccgat ctggtcgatt cggttgcgga tggtttcgat ggcccggtcg acggcgtcga
     5821 gccgctggtc ggggggaagg ccttccgtct tgacctcgta cacgagatgc gcgccgccct
     5881 ggaggtcgag gcccaggttc agtgtctgcg cgcgaagctt gtcgtgcttg tccttgggca
     5941 tcgccgcctg ggcggccttg ctcatggagt agtactggta cgtcgggtac atgacgatcc
     6001 cgaacgcaat cgtgacaagg gcaataaaaa gcccgcgcca ggctgtcttg gacatggcaa
     6061 ctcccggatg gactaatcgg aaggaaaaac gcgagcgggg attgtagaga gaaggggcgc
     6121 ttctgtcaat cagcgagacg ggttggcgcc cttccccgga atcgccacaa cttccgaaac
     6181 gggcacgact tcgagccccg atgccgccca ggccgggagc atttttcgca gggcttccac
     6241 ggtgcccggg tagggacgaa gaatgcccac ggccacaccc cggctccggg ccgtttcggc
     6301 cagctcggcc agctttgcct ccacctgggc ggccgtggcg gtggggtaat ccagcgcccc
     6361 cggactcctc accttccgac tcgggacgcc ggccgccgcc gccacctcct ccacggtgga
     6421 gaacgtgctc ctggtggagt cgaggaaata gccgcgcctc ttcttcacct cgccgatgat
     6481 cgcggtcatc acgctccgat cctcgactgc caggttccct cccagcgtgt ggaccccctc
     6541 ggcgcccccc aggagcttgt aggcctcccc gactcgtttc atgatctccc gctccgtcag
     6601 gtccaccagg atccccttcg gccccggatc gttggtcgga tagttcaggt cttccatcgg
     6661 caggtcgacg agcacctcga tgccgacacg ccgacaccca tccgccaggt cccgggctcc
     6721 cgtggtaccg ggagggatgg ctacggtgat ctcccgggca aaatcgatga tctcgcggga
     6781 ctccttcccc tggacgaaat catggaccag gagagccagc cggggccggc cgtcggtcct
     6841 ggcgtcgggc accaggaaga ccgcctcgac ctggacgtcc gcgcgaagcc gcgtggcgaa
     6901 acccgagttc cccccctcgg gagccggctg cggcttgccg tggaacggaa ccgcggccgc
     6961 cgccaggagg gtggatgccg aatcggcgga caagccccgc ggcaacggca cttcccagcg
     7021 ttggtggagc gagcccttct cgccggcggc gagtgactcc acccggacca gtgtcgtatc
     7081 gaccccgacc ggggcgaggg ccgccttgag cgattcgccg aacagccgcg ccagttcggt
     7141 ggcgggtacc tggggatcgg ccgtggccgt catcacccgc gccccgaaga ggagccggcg
     7201 gcccgccggc atcaccagga aaccgaccag gcccaccagg gccagaatga gaatcacccc
     7261 gagtgagcgg ccaagaccgc cccctcgggg tgatgaaaag gtgcgccggg gaatcagttg
     7321 gccttgagtc cgctgaccga cccgggcgca tcggggtcgc cttcgtcacg ccactcgacg
     7381 agatacatca gcgtgtcggc cggacccgac ggcggatagg tgcgtccctt gcggaaatag
     7441 aagcgctgcg tctggtcctc cacgcggagg gtcgtaacgg gttggccagg agtctgcacg
     7501 tcaacatcaa ggaagacgtc ggtcaggaag agcttggtcc acccctcacg cggcgtgagg
     7561 taccccgcgc ccgaggccga cctctttggc caggtgagtt cgatcttgat cgacgtgacg
     7621 tcgctcgagt tgaacaggac ctcggtctgg aggctgtcgg tcgtccgcga ccagaactcg
     7681 atgccgagct gcccgcgcgt cttcggatcg aaatagaact ggaagtcgct ggcgaggagc
     7741 tccgcgtact taccgatctg ccgctgccgg taagccacct gcaggttgtt gatgacgttg
     7801 tcctcgctcg tctgcggctc gtaccggccg ggatcctggg ggccgtcatc gatttccgtg
     7861 caaccgtaag tccccgtaag ggccagggct ccgaaaagca gcccggcggt cgtgagccgg
     7921 gcggtcctga atgaacgcat gaacctgatt cctcctgaaa atggacggag cgattgtatc
     7981 agccggatgt cgtgtcacgc acggaatatc tggggctatc cattggcgat acggtcgtct
     8041 cctcggccca taacagtcat gtttgaaatc acttaagtat atccccgggc gccgatacgc
     8101 ccgcgtagaa gcccatgtag tagccgtagg tttccgtgcg aagctggtcc acgtcgaaat
     8161 cctcccacgc cacgatcgcc cagttcgtgc ggcgtttgaa ccggaggttc acctctccct
     8221 tgatggaatc gatggccacg gactgcccct gtggaaagac cagcagcttg tagggaagac
     8281 ggttgtagaa ggcgctgtcg gctgtctcgg gcaccagcgg cgtggacgtc gtgtcgaaca
     8341 ggacagacag gtcattcgct ccgatccgca gcgagaggaa caggcggttc gccgtttcga
     8401 attcgtcctt gaaaatgacc tggagaccgt tcaggcgatc ggaatcccgc tcgtccaggt
     8461 ccaggatgaa atccgggtcg atcgtctcgg cgagctgcgg ggcgccgagg accttttcct
     8521 gcagcgcctg ctggaagttg aacagcacgc tctccggcgc cgtgggcgcg tgtcgatccg
     8581 gggggtcgcc gggctcatcg gggtcgcgcg gcgcgaaata gccgcagccg gccagcgtga
     8641 gggtggcaag ggccgcgaac atggccgcat gacggcgggc attgtctctc atc
//
LOCUS       DASVOD010000211         3179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_848390, whole genome shotgun sequence.
ACCESSION   DASVOD010000211 DASVOD010000000
VERSION     DASVOD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848390"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..403)
                     /locus_tag="VF720_04555"
     CDS             complement(89..403)
                     /locus_tag="VF720_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878653.1"
                     /translation="MNRRQMIALFAFPTLVTSLGIASVQLTGAAPQANAAGGSCCDPT
                     CCLTSAGTVMVAAQLAPTADPAIACAIPTAAPAATSAGGCCDAGSCCAVSCEAPAESP
                     AR"
     gene            complement(513..944)
                     /locus_tag="VF720_04560"
     CDS             complement(513..944)
                     /locus_tag="VF720_04560"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7878654.1"
                     /translation="MSGEVRIKPIVDVCVVAGDKVLLVKYGEGAGHDGQAGWFLPDDL
                     MADFEAPDAAAVRCLAEQTGVRTTAPFLSHVESFRGNDGSWHLAFHFVVQLPKAPRLT
                     PSAGIAETRWFDLIGLPAKRDVAHGGWALATIKEILQRASG"
     gene            complement(941..2047)
                     /locus_tag="VF720_04565"
     CDS             complement(941..2047)
                     /locus_tag="VF720_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7878655.1"
                     /translation="MINLLDISSRTVRALRREWILKQGGILSATFGMTYKCNLRCTTC
                     GVWVRGETDTMVELTTAEILEAARQLAELKVPQVILVGSEPLLRRDSLDIIDGMRKLG
                     LDVDVITNGTLFTERTSERVVKAGVRAITVSVDGVGKRHDVVRGQERAWERAMTGLER
                     LAAVKKRLNSNRPRLALHATISRLNADQLEELSALQDSLNMDVSFQLASETSQAEVSG
                     STLDGHVVAGEEFVSRDGSFLPTDDDVRRIRAFVDRPGIIRKHPTLAILATMSDDDIR
                     HGRFPIHQCRVVEGEILIDPYGFVRPCSHLEAYDYGNVREQSIREIWNGLKRRKLVEK
                     VRSDMFPICDKCCQHGQNLSFGQKARMILTKGAG"
     gene            complement(2073..>3179)
                     /locus_tag="VF720_04570"
     CDS             complement(2073..>3179)
                     /locus_tag="VF720_04570"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX7878656.1"
                     /translation="TLASLGLGWVGEPAFAHLFEPFFHGFLKEAMAHTAGLTTAFVLI
                     TFLHIVFGELAPKSLAIQRAEATTLAVARPIIAFYWVFYPLIWSLNGLANLTLKLLRL
                     GPASEHDTTHSEEEIQMLLAHSHAGGRIPVVRQRLLQNVFTFGRRTARQIMIPRTDVI
                     VLSIDRPFAEIVEQVRASGHTRYPVCEGDLDKVLGILHIKDLPTSDPGRKGLEAVLRT
                     PLFVPESMGAERLLLNFQEERQHMAIVVDEYGGASGILTLEDVIEELVGEVQDEFDEE
                     APQLLKVRQGGYLVSAGMLLDAVAAQLGVTLEEEVDADTIGGYVQLKLGTLGRVGDQV
                     PFGRYAIRVVETRGRRLIRLLILPVGAAAAPVGR"
BASE COUNT          518 a         1088 c         1061 g          512 t
ORIGIN      
        1 cgcaccttgc tggcaatgaa gccgtgcagg ccgctccgca tcgagcggta cagctcctcc
       61 aggtccctgg cgggcggcgc gtcgggtgtc atcgagccgg cgattccgcc ggagcctcgc
      121 agctcaccgc gcagcacgaa ccggcgtcac agcagccacc ggccgacgtc gcggccgggg
      181 cggcggtcgg gatggcgcag gcgatggccg ggtcggcggt cggggcgagc tgagcggcca
      241 ccatcaccgt tccagcactc gtcaggcagc aggtcggatc gcagcaggaa ccaccggcgg
      301 cgttggcctg gggcgcggca ccggtgagct ggacggaggc gattcccagg ctcgtcacga
      361 gcgtggggaa ggcaaacagg gcgatcatct gacggcggtt catttcgagg ctcctcaggg
      421 ctactggcga gggcgcctca cggaacctcc gggacacctc gccgttcacc ggaactgacg
      481 ccgcctgccg cgacaggacg caggaaaaat cgctatccgg aggcccgctg gaggatttcc
      541 ttgatggtgg ccagcgccca ccccccgtgg gccacgtccc gcttggccgg caggccgatc
      601 aggtcgaacc accgggtctc cgcgatgccg gcggacgggg tcagcctggg cgccttgggg
      661 agctgtacca cgaaatggaa ggccaggtgc cagctcccgt cgttgccgcg aaacgactcc
      721 acgtgggaga ggaacggcgc cgtcgtcctg acgccggtct gctcggcgag acatcggacg
      781 gccgccgcat ccggggcctc gaagtcggcc atcaggtcgt ccggcaggaa ccatcccgcc
      841 tggccgtcat gcccggcacc ctcgccgtac ttcaccagga ggaccttgtc tcccgccacg
      901 acgcacacgt cgacaatcgg cttgatccgg acctcgccgc tcatcccgcc cccttggtca
      961 ggatcatccg cgccttctgg ccgaatgaca ggttctggcc atgctggcag cacttgtcgc
     1021 agatcgggaa catgtcgctg cgcaccttct cgacgagctt ccggcgcttc aggccgttcc
     1081 agatctcgcg gatcgactgc tcgcgcacgt tgccgtagtc gtacgcctcg aggtggctgc
     1141 agggccgcac gaagccgtag ggatcgatga ggatttcacc ctccaccacg cggcactggt
     1201 ggatgggaaa gcggccgtgc cggatgtcgt cgtccgacat ggtggcgagg atggccagcg
     1261 tcgggtgctt gcggatgatg ccggggcgat ccacaaacgc gcggatccgc cgcacgtcgt
     1321 cgtcggtcgg caggaagctg ccgtcgcggc tcacgaattc ctcgcccgcc accacgtggc
     1381 cgtcgagcgt gctccccgat acctcggcct gcgatgtctc gctggccagc tggaacgaca
     1441 cgtccatgtt caggctatcc tgcaacgccg acaactcctc gagctggtcg gcattcagcc
     1501 gggagatggt ggcgtgcaat gccagccggg ggcgattgga gttcaggcgc ttcttcaccg
     1561 ccgccagccg ctcgaggccc gtcatggcgc gctcccaggc gcgctcctgc ccgcgcacca
     1621 cgtcgtgcct cttgccgacc ccgtccaccg ataccgtgat ggcccgaacg ccggccttca
     1681 cgaccctctc ggacgtccgc tcggtgaaaa gggtgccgtt ggtgatgaca tccacgtcga
     1741 gtcccagctt gcgcattccg tcgatgatgt cgagggagtc gcgccggagg aggggttccg
     1801 atcccaccag gatcacctgg ggcaccttga gctccgccag ctggcgggcc gcctcgagga
     1861 tttcggcggt ggtgagctcg accatcgtgt cggtttcgcc ccgaacccac actccgcagg
     1921 tcgtgcagcg gaggttgcac ttgtaggtca tcccgaaggt ggccgacagg atgccgccct
     1981 gcttcaggat ccattcgcgg cgcagggccc ggacggttcg ggaggagatg tcgaggagat
     2041 tgatcacggg caacgattat accgcaggcg cgtcagcgcc ccaccggggc cgcggccgcc
     2101 ccgaccggca ggatcaggag ccggatcagc cgccggccac gggtttcgac cacccggatg
     2161 gcgtaccgac cgaacggcac ctggtcgccg acgcgcccca gcgtgcccag cttcagctgc
     2221 acgtagccgc cgatggtgtc ggcgtcgacc tcctcctcga gcgtgacgcc gagctgggcc
     2281 gccacggcgt cgagcagcat gccggcgctc accaggtagc ccccctgacg caccttgagg
     2341 agttggggcg cttcctcgtc gaattcgtcc tgcacctcgc cgaccagttc ctcgatgacg
     2401 tcctcgagcg tgaggatgcc ggaggccccg ccgtactcat ccaccacgat ggccatgtgc
     2461 tggcgctctt cctggaaatt caggaggagg cgctcggcgc ccatcgattc ggggacgaag
     2521 agcggggtgc ggagcaccgc ctcgagcccc ttgcgccccg ggtcgctcgt cggcaggtcc
     2581 ttgatgtgca ggatgccgag caccttgtcg agatccccct cgcacaccgg atagcgggta
     2641 tggccggaag cgcgcacctg ttccacgatc tccgcgaatg gacggtcgat ggaaaggacg
     2701 atgacatcgg tcctcgggat catgatctgt cgggccgtcc gccgcccgaa cgtgaagaca
     2761 ttttgtaaca gtcgctggcg caccaccggg atccttccac cggcgtggct gtgcgccagg
     2821 agcatctgga tctcttcctc gctgtgcgtc gtgtcgtgct cggaagccgg tccgagccgc
     2881 aggagcttca gcgtgaggtt cgccagcccg ttcaacgacc agataagggg atagaaaacc
     2941 cagtagaagg cgatgatcgg ccgcgccacc gccagcgtgg tggcctccgc gcgctggatc
     3001 gccagcgact tcggggccag ctcgccgaac acgatgtgca ggaaggtgat cagcacgaag
     3061 gcggtcgtga ggcccgccgt gtgcgccatg gcttccttca ggaacccatg gaagaacggc
     3121 tcgaacaggt gcgcgaaagc cggttcgccc acccatccca aaccaaggct ggccagcgt
//
LOCUS       DASVOD010000212         7714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_483959, whole genome shotgun sequence.
ACCESSION   DASVOD010000212 DASVOD010000000
VERSION     DASVOD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483959"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..1137
                     /locus_tag="VF720_04575"
     CDS             40..1137
                     /locus_tag="VF720_04575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7878657.1"
                     /translation="MKPRILMVGPYPPPFGGLGNNMSLLLNSDLSRRYDLDLLRTSKH
                     VKHVKVSQADAWSGPYLVWNSLRMIDRLIRRRPRLVYVKATSDTGFVRDAALMALARM
                     FGLKVVCHLHGRPMGRLFAETGFWPRQVARAMRMASVTVVLSPGLKTIFSRMFPGQPI
                     EVLPNVVDISKIAPPPARNGSGPVRILFVGRLSRDKGAFDILEIAGRLMATDPDFRID
                     LVGIAETEAEEKEIRRRTAELGLADHLVFHGYRSGAEKATLFANADIFLLPTYAEIFP
                     NVVLEAMAAGLPVVTTDVPVIPEMVTENVHGHLRKPGDIDGFVEALRLLVRDPERRRA
                     MGAHNRTEAETRYDVPVAVTTLSTIFDRVMS"
     gene            1134..2462
                     /locus_tag="VF720_04580"
     CDS             1134..2462
                     /locus_tag="VF720_04580"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEX7878658.1"
                     /translation="MSIRKQSALISATSFIQLGFGMAAGILVSRVLGPEGKGISVLAF
                     YLPSFLASLGSLSIGEASTFFLGRGVEARRVVSNTLIAAFGLGILYAAACLIGLNFLQ
                     QKIVPDVPVPLIVAGLAVMPLMLLKNYGDGMLVALGRLSWFIAGNLTLHVSRAIFLFI
                     ALVLMKPSVTGAVAAEFASWILTGILYLVGLVKGNGVDFKVDRALAADQLRYSGITHV
                     GNMAMRANHQLSTGILSALSGSIAVGLFSVATTMAQVLWYLPDSVGRILFPRVAGSTR
                     EEGSRLTAQVCRHTMLLTAAACLALYLAGPWVVLFLNGEKFRGSVMPMNLLLPGVLAS
                     VLNRVLSKYLSGIGKPNLNSMASIVALVVNVPLLYILIGPYGVNGAALATTIAYVVNG
                     ILMLWFFARESGVPWWDCLVPTLDDLKMYPLSIAQVWNRLRRMIRRAEAT"
     gene            2462..3559
                     /locus_tag="VF720_04585"
     CDS             2462..3559
                     /locus_tag="VF720_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7878659.1"
                     /translation="MRIVFVSPKAWLVATRRAGIHGGAELQVTRLARGLAARGHDVVI
                     MTEGEPERPAEAVEGVRVKQAWSAEGGLPFFGIFGQGKSLNAAITAEKPHVVLLRAAS
                     PFVSFLADQARREGHRFLFMVSNDSQLDGRYEATSNFRDRDLWHKGLKKAHGVITQTA
                     DQTKKLKERFGIEGFHVPSAIAPTPDPAPAIIPEHVFWAGRFEEPKRPHLLLEIARKA
                     PELPFKVAGWVPAKSDYAARFLAEAKELPNVELVGRLGYDAMDTTYMKSIALLSTAGP
                     EGICNTFLESWRLGLPVISLTADPDEVICRYDLGLHSDDTGKVVDQLHSLRDDFTRRA
                     IVSGNAWRYMKQHDERAVVDRLETYLKTAVA"
     gene            3573..4688
                     /locus_tag="VF720_04590"
     CDS             3573..4688
                     /locus_tag="VF720_04590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7878660.1"
                     /translation="MMVIHGLAGGGAERVCCHLADAWVARGSRVTILTLDDTAPPFYP
                     LDSRVELMPLARPIGKVSALTLVRHHLEMMGLIRRAIVRAKPDVVISFIDQTNVEVIL
                     ATRGLRVPVLAYEANDPDRGLRAAARNWKLWERLRHWTYPHADRVVILSEDFRNYFRP
                     NVRKRCVVVANPVLPPDDADSTGSPSDGQAPTRRPVVLAAGSFSYQKQFDHLLRAFAI
                     VAPSHPDWSLDLFGKGPEQNRLLALIEELGLSGRVRLPGHTKTLGDEMRRASLFAMSS
                     RYEGFPMVLGMAMAHGLPAISYDCPGTPGEMIRDGIDGYLVPPDDIGGLAAALDRLMS
                     DAALRGELSARAPEVIERFSVKRVMDDWERLIREAQH"
     gene            complement(4728..>7714)
                     /locus_tag="VF720_04595"
     CDS             complement(4728..>7714)
                     /locus_tag="VF720_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX7878661.1"
                     /translation="TLSVGDVSVTEGNSGTTAAELTVTLSAASGQSVTIDFQTADGTA
                     LSPDDYQATTGSLTFAPGETFKTVSVQVGGDLLNETDETFLVGLSNPTNATVSDDQAL
                     VTLLNDDGVPSFAIADASIAEGNAGTTNATFTVTLSAASGQTMAVDFRTTDGTALSLD
                     DYQAAAGSLTFAAGETTKTVSILVVGDLLNESDETCAVDLSNATNATVVDGHALVTIL
                     NDDGVPSLSIADASVMEGNSGTTNATFTVTLSAVSGLPVTVDYETSDGTALDLLDYQA
                     AAGSLTFAPGETTKSIVVAVAGDLLNEADEAFLVQLSNAGHATVSDGEATGAILNDDG
                     VPSLSIGYVSVTEGDSGTKAAQLTVTLSAASGQPVTVDFQTADATAQSPDDYQATSGS
                     LTFAPGETVRTVSVLVVGDLLNETDETIAVDLSNATNATVSEDQALVTILNDDAVPTL
                     MIGDVSITEGNAGSTAAQMTVTLSAAGGQPVTVDFQTADGTALSAEDYLASGGSLTFA
                     PGETTKTFIVSITGDLLNEADEVVRVDLSNPTNATVTDAQGRITILNDDGAPSLSIED
                     ASVTEGNSGTTNATFTVTLSAASGLPVSVDYATSDGTALDPDDYQAAAGSLTFAPGET
                     TKNLAIAVVGDLLNETDEALLVGLSNATNAAVSDDQGLLTILNDDGVPSLSIGDVIVT
                     EGNSGTSAAQLTVTLSAASGQPVTVDYSTVNGSAVAPGDYVVGVGTVTFAPGEVARSV
                     PVLVNGDMLSEASETFVVRLASPVMATILDADGLVTILDDDAAPVLTIGDVTVLEGTG
                     GATSVTLSVRLSPASGQTVSVDWATADDMATAPADYVASGAVLTFAPGDTVKTVTVSI
                     VPDINNEPTEDFFVRLTNPLHAVAGDGEAVVVILDDDAVSGVGEPGNFRTLLAPNYPN
                     PFQERTTIPWSLAEPGSVHLQVFDVSGRLIRTLVDGQRSAGEFRAEWDGRDDAGSPVA
                     RGLYLLSLRTTDGQQVRRLILR"
BASE COUNT         1272 a         2657 c         2417 g         1368 t
ORIGIN      
        1 tctgttacaa ttttcagcgc ggtttccgaa acccacttta tgaagccacg aatcctgatg
       61 gtcgggccct atccaccgcc gttcggcggc ctgggcaaca acatgagcct cctgctcaac
      121 tccgaccttt cccgccggta cgatctcgac ctcctccgca cctccaagca cgtgaagcac
      181 gtgaaggtga gccaggccga cgcctggagc gggccgtacc tcgtctggaa cagcctcagg
      241 atgatcgacc ggctcatccg gcgtcgtccg cgcctcgtct acgtgaaggc caccagcgac
      301 accggcttcg tgcgcgacgc cgccctcatg gccctcgccc ggatgttcgg cctgaaggtg
      361 gtgtgtcatc tccacggccg cccgatgggg agactctttg ccgagaccgg cttctggccc
      421 cgccaggtgg cccgggcgat gcggatggcc agcgtgaccg tggtcctctc gcccggcctc
      481 aagacgatct tcagccggat gttcccgggg cagccgatcg aagtgctccc caacgtggtg
      541 gatatctcga agatcgcccc cccgccggca cgtaacggtt cgggcccggt gcgcatcctc
      601 ttcgtggggc gactgtcgcg cgacaagggg gctttcgaca tcctggagat cgccggcagg
      661 ctcatggcca ccgatcccga tttccggatc gacctggtcg gaatcgccga aacggaagcg
      721 gaggagaagg aaatccgccg ccggaccgcc gagctcggcc tggcggacca cctggttttt
      781 catggctacc gttcgggcgc cgagaaggcc acactctttg ccaatgccga cattttcctc
      841 ctccccacct acgccgaaat ctttcccaac gtggtcctcg aggcgatggc ggccgggttg
      901 ccggtggtga cgaccgacgt gccggtcatc cccgagatgg tgaccgagaa cgtccacggc
      961 cacctgcgca agcccggcga catcgacggc ttcgtggagg ccctccgcct cctcgtgcgc
     1021 gacccggaga ggcgccgggc gatgggcgcc cataatcgga ccgaggccga aacccgctac
     1081 gacgtgcccg tggcggtcac gacgctctcc acgatcttcg accgggtcat gtcttgagca
     1141 tccgcaagca atcggcgctg atctcggcca ccagcttcat ccagctcgga ttcgggatgg
     1201 cggccggcat cctcgtctcg cgggtcctgg ggccggaagg aaagggaatc tcggtcctgg
     1261 cgttctacct gccgagtttc ctggccagcc tgggatcgtt gtcgatcggc gaggccagca
     1321 ccttcttcct cggacgcggc gtggaggcgc ggcgcgtggt gagcaacacg ctcatcgccg
     1381 ccttcgggct cggcatcctg tacgccgccg cctgcctcat cggcctgaac ttccttcagc
     1441 agaagatcgt gcccgatgtg ccggtcccgc tgatcgtggc cggcctggcc gtcatgccgc
     1501 tcatgctcct caagaattac ggggacggca tgctggtggc gctcggccgt ctgtcgtggt
     1561 tcatcgccgg caacctcacg ctgcacgtgt cccgggccat cttcctcttc attgcgctgg
     1621 tgctgatgaa accaagcgtg accggcgccg tggcggccga gttcgcctcg tggatcctga
     1681 cggggatcct ctacctcgtc ggcctcgtga aggggaacgg cgtcgacttc aaggtggacc
     1741 gggccctggc cgcggatcag ctccgctaca gcggcatcac ccacgtgggc aacatggcga
     1801 tgcgggccaa tcaccagctt tccacgggca tcctgagcgc gctttcgggc tcgatcgccg
     1861 tcggcctctt cagcgtggcc accaccatgg cccaggtcct ctggtacctc cccgattcgg
     1921 tcgggcgcat tctcttcccg cgcgtggccg gatcgacacg cgaggaaggg agccgcttga
     1981 cggcgcaggt ctgccgccac accatgctgc tcacggccgc cgcctgcctc gcgctttacc
     2041 tcgccgggcc gtgggtcgtg ttgttcctga acggcgagaa gttccgggga tcggtgatgc
     2101 cgatgaacct cctcctcccg ggcgtcctgg ccagcgtcct gaatcgcgtc ctctccaaat
     2161 acctctccgg gatcggcaag ccgaacctca actcgatggc gtcgatcgtg gcgctcgtcg
     2221 tgaacgtccc gctcctttat atcctcatcg gcccgtacgg cgtgaacggg gctgccctgg
     2281 ccaccaccat cgcctacgtc gtcaacggca tcctcatgct ctggttcttc gcgcgcgaat
     2341 ccggcgtccc ctggtgggac tgcctggtgc cgacactcga cgacctgaag atgtatcccc
     2401 tctcgatcgc acaggtgtgg aaccgccttc gccggatgat ccgccgcgcc gaggccacct
     2461 gatgcggatc gtcttcgtga gcccgaaggc ctggctcgtg gcgacgcgga gggcgggaat
     2521 tcacggcggg gccgagctcc aggtgacgcg gctggcccgt gggctggccg cccgcgggca
     2581 cgacgtggtg atcatgaccg aaggggagcc cgagcggccg gccgaggccg tggaaggcgt
     2641 tcgggtgaag caggcctggt cggccgaggg gggactccct ttcttcggca tcttcggcca
     2701 gggaaagtcg ctcaacgccg ccatcaccgc cgaaaagccc catgtggtgc tgctccgcgc
     2761 cgcatccccc ttcgtcagtt tcctggccga ccaggcgcgc cgcgaggggc accgtttcct
     2821 cttcatggta tcgaacgact cgcagctgga tggacgctac gaggccacct cgaatttccg
     2881 ggaccgtgac ctgtggcaca agggcctcaa gaaggcgcat ggcgtcatca cccagaccgc
     2941 cgaccagacg aagaagctca aggagcgatt cggcatcgag gggttccacg tgccgagcgc
     3001 catcgcaccc acgcccgatc cggcgccggc catcattccc gaacatgtct tctgggccgg
     3061 ccgcttcgag gagccgaagc gcccgcacct cctgctggag atagcgcgga aggcgcccga
     3121 actccccttc aaggtggcgg gatgggtgcc tgcgaagagc gattacgccg cgcgcttcct
     3181 ggcggaagca aaggagctcc ccaacgtgga actggtcggg cggctgggct acgacgccat
     3241 ggacaccacg tacatgaagt cgatcgccct gctctcgacg gccgggcccg agggcatctg
     3301 caacaccttc ctggaatcgt ggcggctcgg cctgccggtc atctccctga ccgcggaccc
     3361 cgatgaagtg atatgccgct acgacctcgg gcttcacagc gacgacaccg gcaaggtggt
     3421 cgatcagctt cactcgctgc gcgacgactt cacccggcgc gccatcgtgt cggggaatgc
     3481 ctggcgctac atgaagcagc acgatgagcg ggcggtcgtg gatcggctgg agacttacct
     3541 gaagaccgcg gtggcctgac ccgatgcacg tgatgatggt catccacggc ctggccgggg
     3601 ggggagcgga acgcgtctgc tgccacctgg ccgatgcctg ggtggcacgc ggctcgaggg
     3661 tgacgatcct gaccctggac gacaccgcgc cccccttcta cccgctcgat tcccgggtgg
     3721 aactgatgcc gctggcccgt ccgatcggaa aggtgtcggc gctcacgctg gtccggcacc
     3781 atctcgaaat gatggggctg atccgccgcg ccatcgtccg ggcgaaaccc gacgtcgtca
     3841 tctccttcat cgaccagacg aatgtggagg tgatactggc cacccggggc ctccgggtac
     3901 cggtactggc atacgaagcc aacgatcccg accggggact gcgcgccgca gcccgaaact
     3961 ggaaactgtg ggagcgcctg cggcactgga cctacccgca tgccgatcgg gtggtgatcc
     4021 tctccgagga ctttcggaat tacttcaggc cgaatgtccg gaaacgctgc gtggtggttg
     4081 ccaacccggt gctccccccg gacgatgcgg actccaccgg gtcaccatcg gatggccagg
     4141 ccccgacccg gcgccccgtg gtgctggccg ccggatcgtt ctcctaccag aagcagttcg
     4201 atcacctgtt gcgtgccttt gccatcgtgg ccccctccca ccccgactgg tcacttgacc
     4261 tcttcggcaa ggggcccgag cagaatcgcc tgctggccct gatcgaggaa cttgggctct
     4321 ccgggcgcgt gcgcctcccg ggtcacacga agacgctggg tgacgagatg cgccgtgcca
     4381 gtctgtttgc catgtcctcg cgctacgagg gcttcccgat ggtgctcggc atggccatgg
     4441 cccacgggct gccggcgatc agctacgact gccccggcac gccgggcgag atgatccggg
     4501 atggcatcga cgggtacctc gtgccgccgg atgacatcgg gggactcgcg gcggccctgg
     4561 accggttgat gtccgacgcc gcccttcgcg gggagttgtc ggcccgcgct cccgaagtaa
     4621 tcgaacgatt cagcgtgaag cgcgtgatgg acgactggga gcggctgatc cgggaggcgc
     4681 aacactgaaa cccggcaacc cgacagttac cggggccccg caccaactca gcgcaggata
     4741 agccgccgca cctgctggcc gtccgtcgtc ctcaggctga gcaggtagag tccacgcgcg
     4801 accggactcc cggcgtcatc gcgcccatcc cactcggccc ggaattcgcc cgccgacctc
     4861 tgcccgtcca ccagtgtccg gatcaatcga cccgacacgt cgaacacctg gaggtggacc
     4921 gagcccggct ctgccagcga ccagggaatc gtcgtccgct cctggaacgg gttcggatag
     4981 ttgggcgcca ggagggtgcg gaagttcccc ggctccccga caccgctcac cgcatcgtca
     5041 tcgaggatca cgacgacggc ctcgccgtct cccgccaccg cgtgcaacgg attcgtcagc
     5101 cggacaaaga agtcctcggt cggctcattg ttgatgtccg gcacgatcga caccgtgacc
     5161 gtcttcaccg tatctcccgg tgcgaacgtc aggaccgcac cggacgccac atagtccgcc
     5221 ggagccgtgg ccatgtcatc cgcggtggcc cagtccacgg acaccgtctg cccgctggcc
     5281 ggtgacagcc ggacgctgag ggtcacgctc gtcgccccac ccgtcccctc gagcacggtg
     5341 acatccccga tcgtcagcac cggcgccgca tcgtcgtcca ggatcgtaac cagcccatcg
     5401 gcatccagga tcgtggccat gaccgggctc gcaagccgca ccacgaacgt ttcactcgcc
     5461 tcgctgagca tgtcgccgtt caccagcacc ggcaccgacc tggccacttc acccggcgca
     5521 aacgtgaccg tccccacccc caccacataa tcccccggcg ccaccgccga cccattcacc
     5581 gttgagtaat ccaccgtcac gggctgtccg ctggccgccg acagggtgac ggtcagctgg
     5641 gcggcactcg ttccggagtt gccctccgtg acgatgacgt ccccgatgga caggctcggc
     5701 acgccatcgt cattcaggat ggtgagcagt ccctggtcgt ccgaaacggc ggcgttggtt
     5761 gcattggaca gcccaaccag gagcgcctcg tccgtctcat taaggagatc gccaacaacc
     5821 gcgatcgcaa ggttctttgt cgtctcgccc ggagcaaagg tcagcgatcc agcggcggcc
     5881 tggtagtcat caggatccag ggcggtgcca tccgacgtcg catagtcgac cgacaccggc
     5941 aggccgctgg cggccgacag cgtgaccgtg aacgtggcat tcgttgttcc ggagttgccc
     6001 tccgtaacgc tggcgtcctc aatcgacagg ctcggggcgc cgtcgtcgtt caggatcgta
     6061 atccggcctt gggcgtccgt caccgtggcg ttggtgggat tcgagaggtc aacacgcacc
     6121 acttcgtcgg cttcattcag gagatcaccc gtgatcgaca cgataaacgt cttggtcgtt
     6181 tcgcctggcg cgaacgtcag ggagccgcca gatgccaggt agtcctccgc ggacagcgcc
     6241 gtgccgtccg cggtctggaa gtcgaccgtc acgggctgtc cgccggccgc cgacagcgtg
     6301 accgtcatct gggccgcagt cgacccggcg ttaccttccg ttatgctgac atccccgatc
     6361 atgagcgtcg ggacggcgtc gtcattcagg atcgtgacca gcgcctggtc ctccgaaacg
     6421 gtggcattcg tcgcattgga gaggtcgacc gcgatcgtct cgtcggtttc attgagaaga
     6481 tctcccacga ccaggacact gacagtcctg acggtttcac ccggcgcgaa ggtcagagag
     6541 cctgaggtcg cctgatagtc gtcgggtgac tgcgccgtgg cgtcggcagt ctgaaagtcg
     6601 accgtcacgg gctgtccgct ggccgccgac agggtgaccg tcagctgggc cgccttcgtc
     6661 cccgagtcgc cttccgtgac gctgacgtag ccgatcgaga gacttggcac cccatcgtca
     6721 ttcaggatag cgcccgtggc ttcgccgtca gaaacagtcg catggcccgc gttactcaat
     6781 tggaccagga acgcctcatc cgcctcattc aggagatcgc cggcaacggc gaccacaatg
     6841 ctcttcgtcg tctcgccagg cgcaaaggtc agcgagccgg cggcggcctg gtagtcaaga
     6901 agatcaaggg cggtgccatc cgacgtctca tagtcgaccg tgaccgggag gccgctgaca
     6961 gccgacagcg tcaccgtgaa cgtggcgttc gtcgttcctg agttgccttc catgacgctg
     7021 gcgtccgcga tcgagaggct cgggacaccg tcgtcgttca ggatcgttac cagcgcatga
     7081 ccatccacaa cagtggcgtt agttgcattg gagaggtcga ccgcacacgt ctcgtcggat
     7141 tcattcagaa gatctcccac gaccagaatg ctgacagtct tggtggtttc ccctgccgcg
     7201 aaggtcagag agcccgccgc cgcctgatag tcgtccagcg acagagccgt gccgtcggtg
     7261 gtccggaagt cgaccgccat ggtctgtccg ctggcggccg acaacgtgac cgtgaacgtg
     7321 gcattcgtcg tgcccgcgtt gccttccgca atgctcgcgt ccgcgatcgc gaaactcgga
     7381 acaccgtcgt cattcaggag cgtgaccagc gcctggtcat ctgaaacggt ggcgttggtc
     7441 ggattggaga ggccgaccag gaacgtctcg tccgtctcat tgaggaggtc tccgccgacc
     7501 tggacactca cagtcttgaa ggtttcaccc ggcgcaaagg tcagtgaacc cgtcgtcgcc
     7561 tggtagtcat ccggcgacaa cgccgtgccg tccgcggtct ggaagtcgat cgtcacgctc
     7621 tgtccgctgg ccgccgagag cgtgaccgtc agctcggccg cagtcgttcc ggagttgcct
     7681 tccgtaacgc tgacatcccc gaccgatagc gtcg
//
LOCUS       DASVOD010000213         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_171309, whole genome shotgun sequence.
ACCESSION   DASVOD010000213 DASVOD010000000
VERSION     DASVOD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171309"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VF720_04600"
     CDS             <1..1477
                     /locus_tag="VF720_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878662.1"
                     /translation="MLVDKRVVTGGGPGTPPVERGWAVWLDGMQSYLSIATGGSPEVY
                     LGPILQASTWTHLVVSVDHATHEGRWYKDGIPESAFDFTPLDGFVGNTGDLTMGQPNP
                     GFGFGNVLHGCVSDVALFNAPISADAAYKAWLPGPIGVFCPQYALMPSLTNFCPNQTS
                     KQVCFKIANNTAQSHTYHWSLAPLPAGPGCTVAGPTLMTPSSGVVTVGAGSTSANICV
                     TIQRPAGMTAQGATACFQLTFVNDQTGVCRTKTSKLRADNTCWCATPTQASVVGVPAR
                     SIAGATIGIGIELPCDPVARTYQWRAVWLDDEHPDPQGIRLNGLPPGEPVIGTLTAHA
                     GGSEVVSVNVSYDGVYDITRLYEIRLEADIDGDGQYDPVDPIVVHPLSDTPVSGVDPP
                     VVVVPVAPPVPVLGLSPNPFVGRTTIAFAVPVAGDVDLGIYDLAGRLVRRLVHERLEA
                     GDHRFEWDGRNDGSAKVSGGVYFVRLLANGTRVDGKIVMVE"
     gene            complement(1501..>2781)
                     /locus_tag="VF720_04605"
     CDS             complement(1501..>2781)
                     /locus_tag="VF720_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878663.1"
                     /translation="GGLAEGFVDLANAVVGLVVEPNGRISVASDGCFVARYLPDGSPD
                     FDFGFQGIGGYSKCVEATGFARQPGGGLLLCGRSDDRMAVARMKDDGTADDGFGTDGL
                     AGVDDWPISNVAALRVLPDGRFALAGSGTPEIATHAALARFLSNGAPDPSFGMNGHRL
                     VEIGEESAFSAVVDQPDGRLVAAGFRTNGLGRNLVLFRCKVNGDPDPTFGEAGFVFPA
                     LDFDPDQPLALAWEKDAKIVVAGARQTLVPDAWGGLIPTHEFVAAAFNANGTLDASYG
                     TGGVMVVDLGDRTDDLPRSVAFDPLERAVIAGASSGFIGIVRLRTGATTSVPDGVADR
                     APFELGQVVPNPMRGRAALDLVLSNSARVRADVFDPTGRRVRALVEDELRAGSHVISW
                     DGRDDAGRPVSGGVYFVRVDAGGVPCVRRVTVVE"
BASE COUNT          457 a          994 c          918 g          412 t
ORIGIN      
        1 catgctggtg gacaagcgcg tcgtgaccgg cggcgggccg ggtacgcccc ccgtcgagcg
       61 gggctgggcg gtgtggctcg acggcatgca gagttacctg tcgatcgcca cgggaggttc
      121 gccggaggtg tacctcgggc cgatcctcca ggcctccacg tggacgcatc tcgtcgtgtc
      181 ggtcgatcac gccacgcacg aaggtcgctg gtacaaggac ggcatccccg aatcggcctt
      241 cgacttcacg ccgctcgacg gctttgtcgg caacaccggc gatctcacga tgggacagcc
      301 caatcccggc ttcggcttcg gcaatgtcct gcacggctgc gtttccgacg tggcgctgtt
      361 caacgctccg atcagcgccg acgccgcgta caaagcctgg ctccccggcc cgatcggcgt
      421 cttctgtccc cagtacgcgc tgatgccgag cctcaccaac ttctgcccca accagacgtc
      481 caagcaggtc tgcttcaaga ttgccaacaa caccgcgcag tctcacacct accactggtc
      541 gctggcgccg ctccccgcgg ggcccggctg caccgtggcc ggccccacac tgatgacgcc
      601 gagttccggc gtggtgaccg tgggggccgg cagcacgtcg gccaacatct gcgtgacgat
      661 ccagcgcccg gcggggatga cggcacaagg cgccaccgcc tgcttccagc tgacgttcgt
      721 caacgaccag accggcgtct gccgcacgaa gacttcgaag ctccgcgccg acaacacctg
      781 ctggtgcgcc acgcccacgc aggccagcgt cgtcggggtg ccggcgcgca gcattgccgg
      841 cgccacgatc ggcatcggca tcgagttgcc ctgcgatccc gtcgcccgca cctaccagtg
      901 gcgcgcggtg tggctggatg acgagcatcc cgatccccag gggatccgcc tgaacgggct
      961 ccctccgggc gaaccggtca tcggcacgct caccgcccac gccggcggct ccgaggtcgt
     1021 gtcggtcaac gtgagctacg atggggtcta cgacatcacg cgcctctacg aaatccgcct
     1081 cgaagccgac atcgacggcg acggccagta cgaccccgtc gacccgatcg tcgtgcatcc
     1141 gctgtccgac acgcctgtgt ccggcgtcga tcctccggtc gtggtggtgc cggtggctcc
     1201 gccggtgccc gtgctcgggc tctcgcccaa cccgttcgtc ggccgcacca ccatcgcctt
     1261 cgcggttccg gtggcgggcg acgtggacct cggcatctac gacctggccg ggcgcctcgt
     1321 ccggcggctg gtgcacgaac gactcgaagc cggcgaccac cgattcgagt gggatggacg
     1381 gaacgacggg tccgcgaagg tgtcgggcgg cgtctacttc gtccggctgc tggcgaacgg
     1441 cacgcgggtg gacggcaaga tcgtgatggt ggagtagcgg acgagcgaaa gctcgctcac
     1501 ttactccacc actgtcaccc gccggacgca cggaacgccg ccggcatcca cgcgcacgaa
     1561 atacacgccg cctgaaacag gccgaccggc atcgtcgcgg ccgtcccacg aaatgacatg
     1621 tgagccggcg cggagctcgt cctccaccag ggcccgcacg cggcgaccgg ttggatcgaa
     1681 cacgtccgcc cgtaccctcg cgctgttcga gagaacgaga tccagcgcgg cgcgcccgcg
     1741 catcggattc ggcactacct gtccgagttc gaatggagcc ctgtcggcca ctccgtcggg
     1801 caccgatgtc gtggccccgg tccgcaggcg cacgatcccg atgaaaccgg acgaggcacc
     1861 cgcgatcacc gctcgctcga ggggatcgaa cgcaacggaa cgaggcaggt catcggtgcg
     1921 atcccccagg tcgacaacca tcaccccgcc cgtgccgtac gaagcgtcca gcgttccgtt
     1981 ggcgttgaac gccgcggcca cgaactcatg tgtcggaatc aggccgcccc aggcatcggg
     2041 aaccaatgtt tgccgcgcgc cggccacaac gatcttggca tccttttccc aggccagggc
     2101 cagcggttgg tcggggtcga agtcgagtgc ggggaagacg aagccggcct cgccgaaggt
     2161 cgggtcggga tcgccgttca ccttgcagcg gaagagcaca aggttgcggc ccaggccgtt
     2221 cgtgcggaag ccggccgcca cgaggcggcc gtccggttga tcgacgacgg cgctgaaggc
     2281 gctttcctcg ccgatctcga ccaggcggtg tccgttcatg ccgaacgagg gatccggcgc
     2341 gccgttcgaa aggaatcgcg cgagggcggc atgcgtggcg atttcgggag tgcccgagcc
     2401 ggccagggcg aagcggccgt cggggagcac gcggagggcg gccacgttcg agatgggcca
     2461 atcgtccacg ccggcaagcc cgtcggtgcc gaagccgtcg tcggccgtgc cgtcgtcctt
     2521 catgcgggcc accgccatgc ggtcgtcgct gcgcccgcac aacaggaggc cgccgccggg
     2581 ctgccgggcg aatccggtgg cctccacaca tttcgaatat ccgccgatcc cctggaaacc
     2641 gaaatcgaag tcggggctgc cgtcggggag gtagcgggcc acgaagcaac catcggaggc
     2701 caccgaaatc cggccgttgg gctcgaccac gaggcccacg accgcattgg ccaggtcgac
     2761 gaacccttcc gcaagcccgc c
//
LOCUS       DASVOD010000214         2792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_222873, whole genome shotgun sequence.
ACCESSION   DASVOD010000214 DASVOD010000000
VERSION     DASVOD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222873"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VF720_04610"
     CDS             complement(<1..826)
                     /locus_tag="VF720_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878664.1"
                     /translation="MKSLILVALCLVWLVTPAEGRPGDRFGELRPEIPDLSTESTPPN
                     PALSSHVFADTVGYGGTFWAPDSMRWEALRDSHWSFDSGVGSSINTGANPNKPVGYHQ
                     QMEGWYGIDQTLPSQPYFRRSSTCAIGGSFSFWAGVTLAEANGLCYATGQGYGNDWSI
                     FLARSFSYAGSGSVAFSFDYALDAEPGFDYAYARVDTTGDGSAPDLVLWTGTGVAAAH
                     LAFTLDPGQEMRSSPGNVIIRFVAQSDGGYSDQDGSYPTTCGLLAVDNVQFGASLST"
     gene            complement(943..>2792)
                     /locus_tag="VF720_04615"
     CDS             complement(943..>2792)
                     /locus_tag="VF720_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878665.1"
                     /translation="NVSLGIYGRSSAPAITTTTFDRFQDNFATNGSISGYSPGRIDQN
                     RIKGASAPNPFTILGDTLNARGDGGNTEVRLVFRVRPGPMINTAILATYSSRWTTEPA
                     LTARYGGVWYSARMDTAEQGGVVSAASWMSTFHEDDPGFSGTDRTADPNDPSRLANDI
                     IPDHLFTPGSRLDYFVTARYRPPDPRNPGGTDWGTDPDTNGARFREVEILPSSFAADT
                     TWNCVLYVDHHDDRDQDQQTLEEAGLQQSLGAGSTNAENTRYDRFDNQTPSSAQFSFG
                     RPSGSPYGCGQGQVFFYKAIVWHSASLTSGVLTKEDAAILNPWLLLRAFGNNNFWGSG
                     EGIMQSMQAAGGTPRSFMNNTLGVLQSCTGIRLANCGGTAIDTTYCIPTSPVAGSWFN
                     SSLLPRGRGNGCPELRLFDRLSLNPSVASARGQLNYVRLVPGMGGSPTDIGFASVANY
                     NEIDVIYHTVLDGMGVGSLRADPGNFIAGCDDVSASLARTDDVLDWFGAPLRCRYPPP
                     NAVVDPPPTQYPPGFRLSLGPAWPNPMTGSARIQFTNAVAGADIRLTIFDVTGRAVRT
                     LHKGEAPAGVHELVWDRTNDEGRPVAGGIYFYRLSAEDFAEARRLVVTP"
BASE COUNT          487 a          870 c          963 g          472 t
ORIGIN      
        1 acgtgctgag cgacgcaccg aactgcacgt tgtcgacagc caggaggccg caggtggtcg
       61 gataggaacc gtcctggtcc gagtaccccc cgtccgactg cgcgacgaac cggatgatga
      121 cgttccccgg gctggaccgc atttcctggc ccgggtcgag ggtgaaggcg aggtgtgctg
      181 ctgcgacacc cgttcccgtc cagagaacga ggtcgggagc tgaaccgtcg ccggtggtat
      241 cgacccgggc ataggcgtag tcgaaccctg gttcggcgtc gagggcgtag tcgaagctga
      301 aggccacgct gccggagccc gcgtaggaaa acgatcgggc aaggaagatc gaccagtcat
      361 tcccgtaccc ctgtcccgtc gcgtagcaca gcccgttggc ctcggccagg gttacgcccg
      421 cccagaacga aaatgatccg ccgatagcgc aggtgctgga gcggcggaag tacggctgcg
      481 acggcagcgt ctggtcgatg ccgtaccacc cctccatctg ctggtggtag ccgaccggct
      541 tgttcggatt ggcgcccgtg ttgatggacg aaccgacgcc agaatcgaac gaccagtgac
      601 tgtcgcggag agcctcccag cgcatcgagt cgggggccca gaacgtgcct ccgtagccta
      661 ccgtgtcggc gaagacgtgc gaagaaagcg cgggattggg tggggtcgat tccgtcgaga
      721 gatcgggaat ctcaggacgc agttcgccga aacgatctcc gggtcgacct tcggccggag
      781 tcaccaacca gaccaggcag agcgcgacga gaatcaagga cttcacggtc gtttcctccc
      841 gcagccgggt gggaatcgat aagtcgatgc tgaccagctc tcgggagtcg gaagtgtcct
      901 ccactcatgg agtgcagcca ccaccaggtt gccgcttgcc cgtcagggcg tgacgaccag
      961 cctcctggcc tccgcgaagt cctccgccga gagacggtag aagtagatgc cgcccgcgac
     1021 cggccgcccc tcgtcgttcg tgcggtccca cacgagttca tggacgcccg cgggcgcctc
     1081 ccccttgtgg agcgtgcgta ccgccctgcc cgtgacgtcg aagatcgtga ggcggatgtc
     1141 ggcgcccgcc acggcattcg tgaactggat gcgcgcggag cccgtcatcg ggttgggcca
     1201 ggccggcccg agcgagagac ggaagccggg cgggtattgc gtggggggcg gatcgacaac
     1261 ggcgttcggc ggcggatatc ggcaacggag cggagcaccg aaccagtcga ggacgtcatc
     1321 ggttcgggcc agtgaggccg acacgtcgtc gcatccggcg atgaaattgc ccggatcggc
     1381 tcgcaggctt ccgacgccca tgccatccag cacggtgtgg tagatcacgt cgatctcgtt
     1441 gtagttggcc acggatgcga aaccgatgtc ggtcggcgag ccacccatgc ccggaacaag
     1501 ccgcacgtaa ttgagctgac cccgggccga agccacgctg gggttgagac tcagccgatc
     1561 gaacaaccga agttccgggc agccgttgcc gcggccacgt ggcagcagac tgctgttgaa
     1621 ccagctgccg gcgacaggcg aggtgggaat gcagtacgtg gtgtcgatgg ccgtgccacc
     1681 gcagttggcg agccggatcc ccgtgcagct ttgcaggaca cccagcgtat tgttcatgaa
     1741 gctcctgggc gttccaccgg ccgcctgcat cgactgcatg atcccttcgc ccgaacccca
     1801 gaagttgttg ttgccgaacg cgcgaaggag cagccaggga ttgaggatgg cggcgtcctc
     1861 cttcgtgagg acgccggatg tcagcgacgc cgaatgccac acgatggcct tgtagaagaa
     1921 aacctgcccc tggccgcagc cgtacggcga gccggatggc ctcccgaagg agaattgcgc
     1981 ggacgacggt gtctggttgt cgaagcgatc gtaccgcgtg ttctcggcat tggtgctccc
     2041 cgcaccgagc gattgctgga ggcccgcctc ctcgagagtc tgctggtcct ggtcccgatc
     2101 gtcgtgatga tccacgtaga gcacgcagtt ccaggtggta tccgccgcga aggacgacgg
     2161 caggatctcc acttcgcgga accgcgcgcc gttggtgtct gggtcggtgc cccagtccgt
     2221 accaccggga ttgcgcgggt cgggcggccg gtagcgggcc gtcacgaaat agtcgagccg
     2281 cgatcccggc gtgaacaggt ggtcggggat gatgtcattg gcgaggcggc tcggatcatt
     2341 ggggtcggcc gttcggtcgg tgccgctgaa gccgggatcg tcctcatgga acgtggacat
     2401 ccaggaagct gccgatacca cgcctccctg ctccgccgtg tccatgcggg ccgagtacca
     2461 cacgccgcca tagcgcgccg tgagcgccgg ctcggtcgtc cagcgcgacg aatacgtcgc
     2521 cagtatcgcg gtgttgatca tgggccccgg ccggacgcgg aagaccaggc gaacctccgt
     2581 gttgccgccg tctccccggg cattcagcgt gtcgccgagg atcgtgaacg ggttcggtgc
     2641 cgaggctccc ttgatccggt tctggtcgat ccgtcccggc gagtagccgc tgatgctgcc
     2701 gttggtcgcg aagttgtcct ggaagcgatc gaacgtggtg gtggtgatgg ccggcgcgga
     2761 ggatcgaccg tagatgccga ggctcacgtt gt
//
LOCUS       DASVOD010000215         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_326822, whole genome shotgun sequence.
ACCESSION   DASVOD010000215 DASVOD010000000
VERSION     DASVOD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326822"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF720_04620"
     CDS             <1..313
                     /locus_tag="VF720_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878666.1"
                     /translation="VAFTDLMRPGLYGWCFFVFAAGLICGLAGWRRVAGDVRLLAGLV
                     AVEFLAVVFLYQQIDPAFGGGTGSYFLNSMKRALFILMPGAAAAGCLLLATRAPEGNP
                     T"
     gene            310..1734
                     /gene="rlmD"
                     /locus_tag="VF720_04625"
     CDS             310..1734
                     /gene="rlmD"
                     /locus_tag="VF720_04625"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEX7878667.1"
                     /translation="MTRPTPPVKVGQEIEVAVNDLAFGGAGVARVQGFTVFVRGGLPG
                     QTVRARIVKRKDGFAEARIEEVLVKSPDEIAPVCRHFGPCGGCLWQNLDYNRQLAAKA
                     GQVRDCLVRLGGIAEPPLTPPLAAPAVYGYRNKMEFSFADRAWDEPDCRFGVGLHVRG
                     RFDKVLNNAECHIAAPWMAEVLATVRELAMASGLPPSQMRTHEGFFRFLVLRDGRNTG
                     ERMAHLITWPAAPGSPEERAVDGLLATVVERHPEMTSLLHGTTGSKASVAYCETWRVV
                     AGRPTIRERLLDHHFEIGPNTFFQTNTAQAERLFTLALELAGGPIDLAWDLYCGVGAL
                     TLPLARQARRVVGAELIEGSIEAARANAIANGVTNADFVAIDMKDALTAEGLVDRSGR
                     LVSGRPGLVLVDPPRDGLHADVTAGLNALRPPVIVYVSCNPSTLARDAARLAEGGYGM
                     DTATPVDMFPQTAHVEMVARFRRG"
     gene            complement(1721..2617)
                     /locus_tag="VF720_04630"
     CDS             complement(1721..2617)
                     /locus_tag="VF720_04630"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX7878668.1"
                     /translation="MGRLDRFRPYLVVAAALGLAAIWGMAAFLKALDPAAFAEQITQH
                     HVTPAAWSPLLARVFVAIEALLVVAHLSLWRPKWVFAASGILLLLFMGATGLAWAGGH
                     TEGCGCFGRLAARHPREVLLEDAGFLALAAVGFRAAAGFGSRRWSGPVLIALTPLALA
                     LPFVGPHLPGDSLVTTFNPGEDLTHLAADDLPQPLDDGKVLLVLMGPECSPCDAGLES
                     LNTIAGTEGAPTVIVVYAGSRNEARSWVLDHVPGFAVGSAPEKVLRQYYRKLPQVGLL
                     DGGRIVKVWRGRVPAPGEITPA"
     gene            complement(2625..>3174)
                     /locus_tag="VF720_04635"
     CDS             complement(2625..>3174)
                     /locus_tag="VF720_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX7878669.1"
                     /translation="PRAQIVITGEDATMTGSLPAVAGPFTRTGSDITFSAAGGAWKLA
                     PEAPLVGEISQPNLLLRKKDYQAAARAYKPKNAAISLIKSVRQPIQITVFFGTWCSYC
                     KHYLPGFMKTIDAAANPAITVTYIGISEDMSEPEAELTKNAVTKTPTFIVSSGGQEIG
                     RIVEKPKDTIEEDLALLLLGGK"
BASE COUNT          484 a         1093 c         1105 g          492 t
ORIGIN      
        1 ggttgctttc acggacctga tgcgccccgg tctctacggc tggtgctttt tcgtcttcgc
       61 tgccggcctc atctgcggcc tggccggctg gaggcgcgtg gccggcgacg tccggctgct
      121 ggccgggctc gtggccgttg aattcctggc ggtcgtgttc ctctaccagc agatcgaccc
      181 cgccttcggc gggggtaccg gaagctattt tctcaactcg atgaaacggg ctctcttcat
      241 cctcatgcca ggcgcggcgg ctgcggggtg tctcctcctg gccacgcgcg caccggaagg
      301 taaccccaca tgacccgccc aaccccgccc gtgaaagtcg gacaggaaat cgaggtagcc
      361 gtgaacgacc tggccttcgg gggcgcgggg gtggccaggg tgcagggctt caccgtcttc
      421 gtgcgcggcg gcctgcccgg ccagacggtc cgcgcccgga tcgtcaagcg gaaggacggc
      481 ttcgccgagg cgcgcatcga ggaggtgctg gtcaaatcgc ccgacgagat cgccccggtc
      541 tgccgccact tcgggccgtg cggcggctgc ctctggcaga acctcgacta caaccgacag
      601 ctggccgcca aggccggcca ggtgcgcgat tgcctcgtcc ggctgggcgg catcgccgag
      661 ccgccgctca cgccgccgct cgccgcaccc gccgtgtatg gctatcggaa caagatggag
      721 ttctcgttcg ccgaccgggc ctgggacgaa cccgactgcc gcttcggcgt gggactccat
      781 gtgcgggggc gcttcgacaa ggtgctgaac aacgccgagt gccacatcgc cgcgccctgg
      841 atggccgagg tgctcgccac cgtgcgcgag ctggccatgg cctcggggct tcccccctcg
      901 cagatgcgca cgcatgaagg cttcttccgc ttcctcgtcc tgcgcgacgg ccggaacacc
      961 ggcgagcgga tggcgcacct catcacctgg ccggcagccc cgggatcacc cgaggaacga
     1021 gcggtggatg ggctgctcgc cacggtggtg gagcggcacc cggaaatgac ctcgctcctc
     1081 cacggcacca ccggcagcaa ggccagcgtg gcctactgcg aaacgtggcg cgtggtggcg
     1141 ggacgtccga ccatccggga gcgactgctc gatcaccact tcgagatcgg gccgaacacc
     1201 ttcttccaga ccaacacggc ccaggccgag cgactcttca cgctggcgct ggaactggcg
     1261 ggcgggccaa tcgacctggc gtgggatctc tactgcggcg taggcgcgct cacgctgccg
     1321 ctcgcacgac aggcccggcg agtcgtgggc gccgagctga tcgaggggtc gatcgaggcg
     1381 gcgcgcgcca acgcgatcgc caacggggtc acgaacgccg atttcgtggc catcgacatg
     1441 aaggacgcgc tgacggccga ggggctggtg gatcgatccg ggcgtctcgt gtcaggccga
     1501 cccggcctcg tgctggtgga cccaccgcgc gacggactcc atgccgacgt gaccgccggg
     1561 ctcaacgcgc tccggccacc cgtgatcgtg tacgtctcct gcaatccctc cacgctggcg
     1621 cgcgacgcgg cccggctggc cgagggtggt tatggaatgg acacggccac gccggtcgac
     1681 atgttcccgc agacggccca cgtggagatg gtggcccgct tcaggcgggg gtgatttccc
     1741 cgggggccgg cacgcggccg cgccacacct tcacgatcct gccgccgtcg agcaggccca
     1801 cctgcggcag ctttcgatag tactggcgca gcaccttttc gggcgccgaa ccgacggcga
     1861 agccggggac atggtcgagc acccagcttc gagcctcgtt ccggctgccg gcgtagacga
     1921 cgatgacggt gggcgctccc tcggtgccgg cgatggtgtt gagcgactcg aggccggcat
     1981 cgcaggggga gcactccggc cccatcagca ccagcagcac ctttccgtcg tcgagcggct
     2041 gcggcaggtc gtcggcggcc aggtgggtca ggtcctcgcc cgggttgaag gtggtgacga
     2101 gcgaatcgcc cgggaggtgc gggccgacga acgggagcgc gagtgcgagc ggcgtcagcg
     2161 cgatcagcac cggccccgac caacgtcgtg agccgaagcc ggccgccgcc cggaacccga
     2221 cggcggccag ggccaggaag ccggcatctt ccaggagcac ctcgcgcgga tggcgcgccg
     2281 ccagtcgacc gaagcaaccg cacccctcgg tgtgcccccc cgcccacgcg agccccgtgg
     2341 cccccatgaa gaggagaagc aggatccccg aggcggcgaa cacccacttc ggccgccaca
     2401 gcgagaggtg agccacgacc agcagcgcct cgatggccac gaagacgcgg gccaggagcg
     2461 ggctccaggc cgccggcgtc acgtggtgct gggtgatctg ctcggcgaag gccgcgggat
     2521 cgagagcctt gagaaacgcg gccatccccc agatggcggc gaggccgagt gccgcggcca
     2581 cgaccaggta cggccggaac cgatcgaggc ggcccacgtc ggttttactt tccgcccagg
     2641 aggagaaggg ccaggtcctc ctcgatcgta tcctttggct tctcgacgat gcggccgatt
     2701 tcctggccgc ccgacgacac gatgaacgtg ggcgtcttgg tgacggcatt tttcgtgagt
     2761 tcagcctcgg gctcgctcat gtcctcgctg atcccgatgt acgtgacagt gatggccggg
     2821 ttggccgcgg cgtcgatcgt cttcatgaag cccggcaggt agtgcttgca gtacgagcac
     2881 cacgttccga agaagacggt gatctggatc ggctgccgaa cgctcttgat gagcgagatg
     2941 gccgcgttct tcggtttgta agccctcgcc gccgcctggt agtccttctt ccggaggagg
     3001 aggttcggct gcgagatctc gccgaccagg ggcgcttctg gcgccagctt ccacgctcca
     3061 cccgcggccg agaaggtgat gtcggagccg gtccgggtga acggaccggc caccgccggg
     3121 agcgatccgg tcatggtcgc atcttcgccg gttatcacga tctgggcccg cggc
//
LOCUS       DASVOD010000216         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_119124, whole genome shotgun sequence.
ACCESSION   DASVOD010000216 DASVOD010000000
VERSION     DASVOD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119124"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VF720_04640"
     CDS             complement(<1..389)
                     /locus_tag="VF720_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006633114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX7878670.1"
                     /translation="MTQEITRRDWLKGIAAGAAALNTIQLVACADAQNTPPANPPAAA
                     PAAAPTGPFTLPALPYAVDALEPHIDAMTMEIHHDKHHAAYVTNLNKALAGHADLARM
                     SIEELLTHLDTVPEEIRNAVRNNGGGH"
     gene            444..938
                     /locus_tag="VF720_04645"
     CDS             444..938
                     /locus_tag="VF720_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7878671.1"
                     /translation="MSGARRATPTLVINDSPACLSVPPDTTLLDALRDHLGLVGTKLG
                     CARGECGACTVHLDGKPVYSCLLLVAECDGASVRTIESLAGREGGAPHPLLAAIVEED
                     ALQCGFCTPGQAMSILALIESNPDPSDEEILEALSGNLCRCGAYPGLVRAARRAAEAM
                     RKRR"
     gene            941..>2327
                     /locus_tag="VF720_04650"
     CDS             941..>2327
                     /locus_tag="VF720_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX7878672.1"
                     /translation="MPRILRAKKEVEGRWSEEIVVVEGDDLAVWPAGSTSIVGAAVPR
                     MDGHDRASGRATYTADVRLAGMWHAVFLRSPHAHAMIRAIDTRKAEAAPGVHGVLTHR
                     NAPKIPWYIDSFLLDPHVRFAGEEVAAVLAETESAARAAANLIDVRYDVLPAVTDPER
                     AIAPGAPKLRDSGNLVGGKAARYERGSVEAGLAGADTRIELVARTAGALHNALESHGS
                     VARWEGDDLVLHDSTQFIFGIRQGIADKLGLTLNRVRVLCPFMGGGFGAKNSVRKYMV
                     IAALFARDSGRPVRAVLDRVEENLAAGHRHETVSHVKLGVSAAGRLTALECITSAGGG
                     AHANGMMPVGGPFRELYACPNVTTEERAAQVNLGPQSAFRAPGYVEGAFALETAIDVA
                     ARELGIDPVDFRRRNIPKLDPAMGIPYSRPSFRKILDTGERAAREWPKLVRQRGDIRR
                     GRGMAAASWGVG"
BASE COUNT          346 a          805 c          813 g          363 t
ORIGIN      
        1 tagtgcccgc cgccgttgtt gcggacggca ttccggatct cctcgggcac ggtgtcgaga
       61 tgagtgagca gttcctcgat cgacatcctc gccaggtcgg cgtgccctgc cagcgccttg
      121 ttgagattgg tcacgtaggc cgcgtgatgc ttgtcatggt ggatttccat cgtcatggcg
      181 tcgatgtgag gctcgagcgc gtcgaccgca tagggaagtg ccggcagcgt gaacggcccg
      241 gtgggcgcgg cggccggtgc ggcggccggc ggattggcgg gcggggtgtt ctgggcgtcg
      301 gcgcacgcca cgagctggat ggtgttcagt gccgcggcgc cggccgcgat tcccttgagc
      361 cagtcgcggc gggtgatttc ctgggtcatt tcgtcctccc ggatctgtgc atcgaccaat
      421 acccggtagt acgatagccc tccatgtccg gcgcgagaag agccaccccg acgcttgtca
      481 tcaacgactc gcctgcctgc ctgtcggtgc cgcccgatac aacactcctc gacgcgctgc
      541 gcgaccacct cggcctggtg ggaacgaaac tgggttgcgc gcgcggggag tgtggcgcct
      601 gcacggtgca cctggatgga aaacccgtct attcgtgcct gctgctggtc gctgagtgtg
      661 atggcgcaag cgttcggacg atcgagtcgc tcgccggtcg cgagggtggc gctccccatc
      721 ccctcctggc cgccatcgtc gaggaagatg cgttgcaatg cggcttctgc acgccggggc
      781 aggcgatgtc gatcctggcg ctcatcgaat ccaaccccga cccgtccgac gaggagatcc
      841 tcgaggcgct ctcgggaaac ctctgccgct gcggcgccta tccgggcctc gtgcgcgccg
      901 cgcgccgcgc ggccgaagcc atgcggaaga ggcgctgacg atgccgcgaa tcctccgcgc
      961 gaaaaaggaa gtggaaggac gctggtccga ggagatcgtc gtcgtcgagg gggacgacct
     1021 cgccgtgtgg cccgctggct cgacctcgat cgtcggggcc gcagtgccgc gcatggacgg
     1081 ccacgatcgc gcctcaggcc gcgccacgta cacggcggat gtgcgcctcg ccggcatgtg
     1141 gcacgcggtg ttcctccgct caccgcacgc gcacgccatg atccgcgcca tcgacacgcg
     1201 gaaggccgaa gccgcgccgg gcgtgcacgg ggtcctcacc caccgcaacg cgccgaagat
     1261 cccctggtac atcgactcgt tcctgctcga tccgcacgtc cgctttgccg gcgaggaagt
     1321 ggccgccgtc ctggcggaaa cggaatccgc ggcccgggcc gccgcaaacc tcatcgacgt
     1381 tcgctacgac gtccttccgg cggtgaccga cccggagcgg gccatcgccc ccggtgcccc
     1441 gaagctgcgc gactccggca acctggtcgg cggcaaggcg gcacggtacg aacgcggatc
     1501 ggtggaagcg gggctcgccg gggccgacac gcgcatcgaa ctggtcgcgc gcaccgcggg
     1561 cgcgctgcac aacgcgctcg aatcgcacgg cagcgtggcc cggtgggagg gggatgacct
     1621 cgtccttcat gactccacgc agttcatctt cggcatccga cagggaatag ccgacaagct
     1681 gggccttacg ctcaaccgcg tccgcgtcct ctgcccgttc atgggtggcg gcttcggcgc
     1741 caagaactcg gtgcgcaagt acatggtgat cgccgccctc ttcgcgcgcg attcggggcg
     1801 gccggtgcgg gccgtcctcg accgcgtgga ggaaaacctc gcggccgggc accgccacga
     1861 aacggtgtcg cacgtgaagc tcggcgtttc cgccgcgggg cgcctcacgg cgctcgagtg
     1921 catcacgtcc gccggcggcg gcgcccacgc caacggcatg atgccggtcg gcggcccgtt
     1981 ccgcgaactg tacgcctgcc ccaacgtgac gacggaagag cgcgcggcgc aggtgaatct
     2041 cgggccgcag tcggccttcc gcgcgccggg ctacgtggaa ggcgccttcg cgctggaaac
     2101 ggccatcgac gtggcggcgc gcgaactcgg catcgatccc gtcgacttcc gccgccgcaa
     2161 catcccgaaa ctcgatccgg cgatgggaat cccctactcc cgaccgagct tccggaagat
     2221 cctcgacacg ggcgagcgag cggcgcgtga gtggccgaaa ctggtccgtc agcgcggcga
     2281 catccggcgc ggccgcggaa tggccgccgc cagctggggc gtggggg
//
LOCUS       DASVOD010000217         5850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_901274, whole genome shotgun sequence.
ACCESSION   DASVOD010000217 DASVOD010000000
VERSION     DASVOD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901274"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VF720_04655"
     CDS             complement(<1..631)
                     /locus_tag="VF720_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878673.1"
                     /translation="MSAPPRESRGRLSHIAMLGAVIALVIAVRLAWVQVRCAEAYKKL
                     AMEQHFERERLAPHRGAILDRNMEPLAYTADNPTLIVDANTLTAAEKGTLARGLAPIL
                     GVEAGQLERKLNRQKGAVELNPKATALTAEDLAGLPAFVKVEHHPKRIYRIGPAAAHL
                     TGYVGVNQEGLDGIEVQWDRYLRGEPGWTTYLRDARGHQQAESLKKPPVP"
     gene            complement(628..1038)
                     /locus_tag="VF720_04660"
     CDS             complement(628..1038)
                     /locus_tag="VF720_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878674.1"
                     /translation="MKRADAVEWRRHRRGTGKRSLRGNWAWGITALLVLATAGMGVVW
                     QKVRYEGDAIRHADLMAMNARLTGELETEKFEFNQHATRATLVPKADQLGLVDVPSDA
                     IVLVSFPDAPAPTNPILDGFVGEALASSGARDGQ"
     gene            complement(1035..1940)
                     /gene="rsmH"
                     /locus_tag="VF720_04665"
     CDS             complement(1035..1940)
                     /gene="rsmH"
                     /locus_tag="VF720_04665"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX7878675.1"
                     /translation="MDGHQPVMVREVLQYLIGEDSRIVVDATVGSGGHASAILDAMGP
                     TGRLIGLDRDARALERAQQILARYGERAVLRQANFRSMADVVGPLVGGGVDGVLMDLG
                     VSSEQLDRPDAGFSYRVDGPLSLAMEPGSHPDAADLLNSLSESELVRILDEYGDVRRA
                     RALARSVLRQRPVATTFDLVKAAEAVGAGRPDELSRIFQALRIAVNDEFQSLEEGLAG
                     LERIVRPGGRVVVISYHSGEDRIVKKFFTPVTLGKPLPWLPADGSAHRWMSLHRGAVK
                     PAADEVAVNVRSRSARLRAAQRAGE"
     gene            complement(2038..2568)
                     /locus_tag="VF720_04670"
     CDS             complement(2038..2568)
                     /locus_tag="VF720_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX7878676.1"
                     /translation="MNNEVPETTFRGTFPCTVDQKRRLTVPSRFAKALTPRAKRTVVA
                     VHGLEGCLSFYPLDWWQVFENELLLAETTDPDIRRGTRRLLASAEDLVFDAQNRITLL
                     PSQLEQAGIRSQAILLGQAERMELWSPERWNAYLNEPGTPSYEQAMVGVGPIMKLTSL
                     KVRNIRAGRAETDGQE"
     gene            2794..4383
                     /locus_tag="VF720_04675"
     CDS             2794..4383
                     /locus_tag="VF720_04675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7878677.1"
                     /translation="MVTGIGLNHYPLVWVDEGWIAEVAAQAARGLPLGSPSHGTLYHY
                     SDRLFWMPPLYFYELGGLFRVAGVDLLAGRWFNVFVGGLSTLALFFFLRRRTGSWAAW
                     FAALFFACDTFVWKSHRTIRFESLLTLFGVLLVIAVTAALEREDEDRPTHGAWFAAGL
                     AAGLLVNVHPNALVWLAAVFAMAFVRRGPRLLRTGGAWMALSLALLMALPYLFYLGTD
                     APNQFANVIGQNSYHLEHGAAEGWAPLREWKRWADFFVFPARLPALLAWLAITGLALR
                     LMAGEGAVPAPGSTPPDHHDSPTPVARQLAPWLAALGTYITLLALLPNKTLLYLAGAT
                     PFVAILGALAWRDDEGGSRPERGAPVERGAPVARGLSVAHGAPVARSILIARGVLIAG
                     MLASLIVNVGILWRSRDCRATQDLAAIRAVLQPDDRVAGTFVTWWATQPVSAALPPRP
                     FHEFSRGASLESVARFDPTVIVLGDRQWEQEVPTRFGPLDRALVADGGPLAARAPGLS
                     LPNSCLGPIRLYALRPADLKR"
     gene            4713..5426
                     /locus_tag="VF720_04680"
     CDS             4713..5426
                     /locus_tag="VF720_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878678.1"
                     /translation="MRLGHNSALTRTPHPRAALILLPHTPRTHDRPELPSLQDRQQFD
                     ECPIQHRRRIAARNLVSQQFLRLAHPGVHGRIGRELDAPAGWSERLDDRSGRGRGRGL
                     AHRRGRGRGHRNVVTRIGQQLLLSPDARCTWTRIRKLSRSERNIRLRRPFRQEDFDLA
                     AGAIPRLHQQFRAGLRIQVGSEHQEPGNVDRSVAQVFVGCRKLSEDPRALNALVGGTL
                     RNVQVQRAVLEHGTITSSR"
BASE COUNT          884 a         2040 c         1858 g         1068 t
ORIGIN      
        1 cgggaacggg cggtttcttg aggctctcgg cctgctggtg tccgcgggca tcgcggagat
       61 aggtcgtcca ccccggctcc ccgcgcaggt agcggtccca ctgcacttcg atgccgtcca
      121 gcccttcctg gttcacgccg acatacccgg taaggtgagc ggcggcgggg ccgatccggt
      181 agatccgctt gggatgatgt tcgaccttca cgaaagccgg gaggccggcc aggtcttcgg
      241 ccgtcagcgc ggtggccttg ggattgagct ccacggcgcc cttctggcgg ttgagcttcc
      301 gctcgagttg cccggcctcc acgcccagga tcggggccag cccgcgcgcg agcgtcccct
      361 tttcggccgc cgtcagcgtg ttggcatcga cgatgagcgt gggattgtcg gcggtgtagg
      421 ccaggggctc catgttgcgg tcgaggatgg cgccgcggtg cggggccagc cgttcacgct
      481 cgaagtgctg ctccatcgcc agcttcttgt acgcttcggc gcaacggacc tggacccagg
      541 ccagccgcac ggcgatcacc agcgcgatga ccgcgccgag catggcgata tgggagagtc
      601 ggccccggct ttcccgggga ggcgcgctca ctgcccgtcc cgtgctccgg aagaggccag
      661 ggcttccccg acgaagccgt cgaggatcgg attggtcggt gccggcgcgt caggaaacga
      721 caccagcacg atggcgtcgc tgggcacgtc caccagccca agctggtcgg ccttcggcac
      781 gagcgtggcc cgcgtggcat gttgattgaa ctcgaatttc tcggtctcga gctcaccggt
      841 caaccgcgca ttcatggcca tgaggtcggc atgccggatc gcatccccct cgtagcggac
      901 cttctgccac accacgccca tccccgccgt ggccaggacg aggagagccg tgatccccca
      961 ggcccagttg ccgcgcaggg aacgcttccc cgtgccccga cgatgccgac gccactccac
     1021 cgcatccgct ctcttcattc tcccgccctc tgcgcagccc tgagccgcgc gcttcggctc
     1081 cgcacgttca ccgccacctc gtcggcggcg ggtttcaccg cgccgcgatg gaggctcatc
     1141 caccggtgag cggagccatc cgccggaagc cacgggagcg gcttgccgag ggtcaccggt
     1201 gtgaagaact tcttgacgat ccggtcctcg ccggagtgat acgaaatgac caccacccga
     1261 ccgccgggcc tgacgatccg ttcgagcccg gccaggcctt cttccaggga ctggaactcg
     1321 tcgttcaccg cgatgcgaag cgcctggaag atccgggaga gttcgtcggg ccgtccggcc
     1381 cccaccgcct ccgccgcctt gaccaggtcg aacgtcgtcg ccacgggcct ttgccgcagg
     1441 acggagcggg ccagtgccct ggcgcgccgc acatcgccgt actcgtcgag tatccggacc
     1501 agctccgatt ccgaaaggga gttcaggaga tccgcggcgt cggggtggct gcccggctcc
     1561 atggccagcg acagcggtcc gtcgacgcgg tacgagaacc cggcgtcggg tcgatcgagc
     1621 tgttccgagg aaacgccgag gtccatcagg accccgtcca ccccgccacc cacaagaggc
     1681 ccgaccacgt ccgccatcga acggaaattg gcctgccgca gtaccgcccg ctcaccgtag
     1741 cgggccagga tctgctgcgc gcgctcaagg gcgcgcgcat cccggtccag accgatcagg
     1801 cgtccggtcg gccccatcgc atcgaggatg gccgaggcgt gtccgcccga tccgaccgtg
     1861 gcgtcgacca cgatccggga gtcttctccg atgaggtact ggagaacctc ccggaccatg
     1921 accggctggt gaccatccac ggttcgtttt cccggtccta ccggttgccg cccttggacc
     1981 gcgacgcctc acctcgcgcc gcgacctgca accggcagga cctgttgctg ccctgtgcta
     2041 ctcttggccg tcggtctccg cccggcccgc gcgaatgttg cggaccttga gcgacgtgag
     2101 cttcatgatc ggaccgacgc cgaccatggc ctgttcgtag gatggcgtgc cgggctcgtt
     2161 gaggtaggcg ttccacctct cggggctcca gagctccatc ctttccgcct gaccgagcag
     2221 gatggcttga gagcgaatgc cggcctgctc gagttgcgac ggaagaagag tgatgcggtt
     2281 ctgggcgtcg aagaccaggt cttcggcgga ggccaggagc cgccgcgtac cccgccggat
     2341 gtcggggtcg gtcgtctcgg cgagcaacag ctcgttctcg aagacctgcc accagtccag
     2401 cggataaaaa gagaggcacc cttcaagacc atggaccgcc acaactgttc gcttggcccg
     2461 gggggtcagg gctttggcaa accgcgacgg caccgtaagc cgtcgcttct ggtccacggt
     2521 gcagggaaag gtgcccctga aagtcgtttc cggtacttcg ttgttcacct gcggtccccg
     2581 cgcgttgatg accgacaagg gtgagagtcc ctttttctgg gaatctgtgg gaagttgtcc
     2641 cacggcggca atctatcatt ccccgaatgc tttgcaagca ctttttttac gccattttcg
     2701 agccctggtg gcaactcgta tcgataaaac atgaatcacc acgtaaataa cttgaaacgg
     2761 tctcttcccc tcctgttagt gattgtttac ctgatggtta ccggaatcgg cctgaaccac
     2821 tatccgcttg tctgggtgga cgaggggtgg atcgccgagg tggccgccca ggcggcgcgc
     2881 ggtctccccc tcggaagtcc atcccacgga acgctttacc actattcgga ccgcctcttc
     2941 tggatgccgc cgctctattt ctacgaattg ggcggactct tccgcgtggc gggcgtggac
     3001 ctgcttgcgg gacggtggtt caacgtcttc gtgggcgggt tgagcaccct ggcgctcttt
     3061 ttcttcctgc gacgccgcac gggatcgtgg gccgcgtggt tcgccgcgct cttcttcgcg
     3121 tgcgacacgt ttgtctggaa gtcacatcgc acgatccgct tcgaatcatt gctcacgctg
     3181 ttcggcgtgc tgctcgtgat tgccgtcacc gcggcactcg agcgcgagga cgaggaccgc
     3241 cccacgcacg gcgcctggtt cgccgcgggc cttgccgccg gcctcctcgt caacgtgcat
     3301 cccaacgcgc tggtgtggct ggccgccgtc ttcgcgatgg cattcgtgcg gcgcggcccg
     3361 cgcctcctcc gcaccggtgg cgcctggatg gccctgagcc tcgcgctgct catggcgctc
     3421 ccctacctgt tctatctcgg cactgacgcg ccgaatcaat tcgccaacgt gatcggtcag
     3481 aactcatatc acctcgagca tggcgccgcc gaagggtggg ccccgctgcg cgagtggaag
     3541 cgctgggccg acttcttcgt gttccccgcg cggctccccg cgttgctggc gtggctcgcg
     3601 atcacgggac tggctctgcg gttgatggcg ggggaagggg cggtgccggc gcccggttcc
     3661 acgccacccg accaccatga ttcacccacg cccgtggctc gccagctcgc gccctggctc
     3721 gccgcgctcg gcacctacat cacgttgctc gcgttgctcc ccaacaagac gctgctctat
     3781 ctcgcgggag ccactccctt cgtggcgatc ctcggggcgc tggcgtggcg cgacgacgag
     3841 ggcggaagtc gcccggagcg cggcgctccc gtcgagcgcg gagctcccgt cgcgcgcgga
     3901 ctatccgtcg cccacggcgc tcccgtcgca cgcagcatcc tcatcgcacg cggcgtcctg
     3961 atcgcgggca tgctcgcgtc actcatcgtg aatgtcggta tcctgtggcg ctcgcgcgac
     4021 tgccgggcca cgcaggacct ggccgccatc cgcgccgtgc tccagcccga cgaccgcgtg
     4081 gccggcacgt tcgtgacctg gtgggccact cagcccgtgt cggccgcgct ccctccccgc
     4141 ccatttcacg aattcagccg cggcgcgtcg ctcgagtcgg tggcgcgttt cgatcccacg
     4201 gtgatcgtgc tcggcgaccg ccagtgggag caggaagtgc ccacgcgttt cggtccgctc
     4261 gatcgtgcgc tggtggccga cggtggccca ttggccgccc gcgcgcccgg cctgtcgctg
     4321 ccgaactcgt gcctcggccc gatccggtta tacgcgttgc ggccggccga cctgaaacga
     4381 taagaggggg ttggttcgcg gagggtcggt tcgttcatga tcgacaactg cccgtccaac
     4441 ggcgcgcccg gcgattgacg tttccgcctt cgccgtcccc cgaaccgccg ccgtccgtcg
     4501 tccgcattgt gtgcccgaca cctcaatgtc aggttctcga tcgtcgtcgg ccctccctcc
     4561 gcgaagggct ccagatggtg aaactccagc atccccttcg caccgcactg tttcccggcc
     4621 ggcgacgtga acgtgcagcg cgccccatcg cgttccgaca cggcccgccg gaccgcgggc
     4681 gggatgtagc ggctacccgg ctccggccgg cgatgcgcct cggccacaac agcgccctca
     4741 cccgcacccc tcaccctcgt gccgccctca tcctccttcc gcacaccccg cggacgcacg
     4801 accgccccga acttccgtcg cttcaggatc gtcagcagtt cgatgagtgc ccgatccagc
     4861 accgccgccg gatcgccgct cggaatctgg tgtcgcaaca gttcctgcgc ctcgcgcacc
     4921 cgggcgtgca tggccgcatc ggccgtgaac tggatgcgcc agcgggatgg agcgagcggc
     4981 tggatgaccg gtcggggcgc gggcgggggc ggggcctggc ccaccgccgg ggccggggcc
     5041 ggggccaccg caacgtcgtc acccgcattg gccaacaatt gctgctgagc cccgatgcgc
     5101 ggtgcacatg gacccggatc aggaagcttt ctcgaagtga acggaacatc cgccttcggc
     5161 gcccattccg ccaggaggat ttcgatctgg cggcaggtgc gattccgcgc ctgcatcagc
     5221 aattccgcgc cggcctgcgc atccaggtgg ggtccgagca tcaggagccc ggtaatgtgg
     5281 atcgatccgt tgcgcaggta ttcgtaggct gccggaaact gtcggaggat ccgcgcgcac
     5341 tcaatgcgct tgtaggcggc actctccgca atgtgcaggt gcaacgtgca gtactcgaac
     5401 atggaaccat aacttcgagc aggtaaaggt gccgcctgtc gaactccgcc agatgcgcga
     5461 cgagcaccgc ggtggttgca ccttctttct gcgcaagcga gacgactcga gatgtgagtt
     5521 cgccgttcga cagcgtgcgt ggcgctcctg gcgcgacttc cagtttcgtt ttcatcacat
     5581 ggttttatca tgcgttgttg acgcggccgt gggtaccgcc ggtcagtcga cgatgacccg
     5641 agggcgcgaa ttcgtctcga tcgagcgagg tcgcgccggc aaggggcctg gtgcgcgcga
     5701 cacgcacgcc acagtcgagc ctcgcaacct cgcccgcgga catcaaattt ttctgtcaac
     5761 aactttctga aaaaaatcat tcgcctgacg aatcaactct ccttgaggag agtaaaacga
     5821 acagcgacgt aattttccgc aatggtgaac
//
LOCUS       DASVOD010000218         1906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_379385, whole genome shotgun sequence.
ACCESSION   DASVOD010000218 DASVOD010000000
VERSION     DASVOD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379385"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>1906
                     /locus_tag="VF720_04685"
     CDS             95..>1906
                     /locus_tag="VF720_04685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7878679.1"
                     /translation="MSFLLLVAAFPAAPAHAELFGSDYTLGPCPEDANLIRAGEFTGD
                     DRQDVLATNGSEFWVIPGRRAGGFDAPILRLSGQSIGSLDVSDINGDGLDDIVALDGN
                     RSPGVFLARRLGGFEEFAPFAGMASSRSMVLGDFNGDGRTDLALAPQDTNLVVIHTRS
                     DDGGWATGISRPGIVDYPYLMSADWDGDGLDDVLWPAPWYDASELYSIRGGPDLAAVS
                     TDRMPLPDDIDQDDRMRFADVNGDGHPELLATSSSDGLQALELRPDGSWVSVWSAVPN
                     GEGYDLSIETIDVDGNGLEDLVAFQRYSSPLWVALSSFGTGLGDLSAQPFPTELMAVA
                     AGNWLDDGPRLAFATRRGHLAILSRREEEMVQTTPLPTRTGLVGLFPATADLDHDGRL
                     DLVLGQLETGEAGSIWLVMAEGGRTFRFHEYETGFPPEAVLAVDVTGDGHVDVVAFST
                     YRGAFRVYPGDGQGALGVGGPIVHGPFGMRTHLAQAGDYDADGDIDIAVPGLGTEIQV
                     FRNSGHGQFGAPEALALPGDVSGIVQAPLAPGSRPELLVLTYTPGEIHNIRLEAGGLV
                     DVRVQESVSRSGLPSLADLDGDCRDDFILQVGFNDRIS"
BASE COUNT          280 a          621 c          648 g          357 t
ORIGIN      
        1 ggccagtcca atgttgacgg atcgaggcgg caaactgttg taccggttga gcctgtggag
       61 gccgcctgcc ggcgccctcc tcacgatgtc attggtgtca ttcctcctcc tggtcgccgc
      121 cttcccggcc gcacctgccc acgccgagct gttcggatcg gactacacgc tgggtccatg
      181 ccccgaagat gccaacctca ttcgtgccgg cgaattcacc ggtgacgatc gccaggatgt
      241 cctggcgacg aacggtagcg agttctgggt gatccccggc cgccgggccg gaggattcga
      301 cgccccgatc ctgagattga gcggacagag catcggatcg ctggacgtca gcgacatcaa
      361 tggcgatggg ctcgatgaca tcgtggcgct ggacggaaac cgctctcccg gtgtcttcct
      421 ggcccgtcga ctcggcgggt tcgaggaatt cgctcccttt gccggaatgg cgtcatcgcg
      481 ttcgatggtc ctcggtgatt tcaatggtga tggccggacc gaccttgcgc tggcgccgca
      541 ggacacgaac ctcgtcgtga tccatacgcg aagcgatgat ggtggatggg cgactggaat
      601 ttcccggccg ggaatcgtgg actatccgta cctcatgtcc gcggactggg acggcgacgg
      661 gctcgacgac gttctgtggc cggcgccctg gtacgacgcg agcgagctct attccattcg
      721 cggcgggccc gatctcgccg cggtgtccac ggatcggatg ccgctgccgg acgacatcga
      781 ccaggatgat cggatgcggt tcgccgacgt caacggcgat ggccaccccg aactgctggc
      841 cacgtcgtct tccgacggac tccaggcact ggagctacgg ccggacggga gttgggtcag
      901 cgtctggtcg gcggtcccca acggggaggg gtacgacctg tcgatcgaga cgatcgacgt
      961 ggatggaaac ggactcgaag acctggtggc gttccagcgt tactcctcgc cgctctgggt
     1021 ggcgttgtcc tcgttcggaa cgggtctcgg tgacttgtcg gcccagccgt ttccgaccga
     1081 gctgatggcg gtggccgccg gcaactggct cgacgacgga cccaggctcg cctttgccac
     1141 gcgccgcggc cacctggcca tcctgtcccg gcgggaggag gagatggtgc agaccactcc
     1201 cttgccgacc cggaccggtc ttgtgggcct ctttccggcc acggccgacc tggatcacga
     1261 cggccggctc gacctggtgc tggggcaact cgagaccggt gaggccggtt cgatctggct
     1321 ggtcatggcc gagggcggcc ggacattccg gttccatgag tacgaaaccg gctttcctcc
     1381 cgaggcggtg ctggcggttg acgtgacggg cgacggacac gtcgatgtgg tggccttctc
     1441 cacctatcgt ggcgccttcc gtgtgtatcc cggcgacggt cagggcgcac tgggggtcgg
     1501 cggccccatc gttcacggtc ccttcggcat gaggacccac ctggcgcagg ccggcgatta
     1561 tgatgcggac ggcgacatcg acatcgccgt cccgggcctc gggaccgaga tccaggtgtt
     1621 ccgcaattcg ggccacggcc agttcggtgc gccggaggcc ctcgcgcttc ccggggacgt
     1681 gtccggcatc gtccaggccc ccttggcccc ggggagtcgg ccggaactcc tcgtgctgac
     1741 ctataccccc ggggagattc acaacatccg cctggaagcc ggggggctcg tcgacgtccg
     1801 cgtgcaggag tcggtgtcgc gctcgggcct cccgtccctg gccgatctcg acggcgactg
     1861 ccgtgatgac ttcatcctgc aggtcggctt caatgaccgg atttcg
//
LOCUS       DASVOD010000219         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_588563, whole genome shotgun sequence.
ACCESSION   DASVOD010000219 DASVOD010000000
VERSION     DASVOD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588563"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF720_04690"
     CDS             complement(<1..447)
                     /locus_tag="VF720_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878680.1"
                     /translation="MPTHGIRATSVAPFVLAAILWPFPAHAQASDRGSVRQRAALDSV
                     ALAVRTLQSRVDSLTAADRDTPLSVASASPGSAPAPRAGGAYMNISFDGLVDFGWSSE
                     ADVRSLEMGDHDPLVRGFTIPNAEISLDGTVDPHFKGFSNIVLKLDE"
     gene            complement(635..955)
                     /locus_tag="VF720_04695"
     CDS             complement(635..955)
                     /locus_tag="VF720_04695"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HEX7878681.1"
                     /translation="MGCSVTAMSWIRTFRHRPLLALILSLLVLATGIVAVGAHHDHDT
                     GHHADCPTCRLADEAAGAVPVVSVTASVILPGAPFAVVLPATSPAVASACDAHRLRAP
                     PQIG"
     gene            complement(979..>2237)
                     /locus_tag="VF720_04700"
     CDS             complement(979..>2237)
                     /locus_tag="VF720_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878682.1"
                     /translation="LWHDRIDPLLVRRGAAIDSLAIALGYGGYTEFAVRLRRTDPERV
                     LPGLFGFIRATDTMYQLLGRELATPVTVVPARVAARRPPSLKSADFRHLPWSDTWDRY
                     VDAAPVLAREWTSLPAAVDPGRPTPVVQAIAPHLVMEGRPAVVDDASDVQAVVVCPNP
                     PVEVKLAVSDVTGMAAARAALAAAGEARQKNGTAESKPEWRDAGPELLPATSAELSML
                     LTSSPEWYEQRRRDDRASGRDTDVTRLSDLHLARLIRWQLWNDLRRIRSEIAGQLVID
                     LIRHRAGTDYWMPYVFAPAGVDEGELIRQIRGFARGLDLTPAESYDALANRGDFLPGL
                     DAARAVALAAAVEEHLRERIGPEWFLDTDLRREAVFATPDGLAAGSEADIAKALAGTD
                     VIDFSALQRRCERLFDWSERVLEVAR"
BASE COUNT          382 a          759 c          809 g          287 t
ORIGIN      
        1 ttcatccagc tttaggacga tgttgctgaa gcccttgaaa tgcgggtcga cagtcccgtc
       61 gagggagatc tcggcattcg gaatcgtgaa gccgcgcacc agcgggtcgt gatcccccat
      121 ctccagcgag cggacgtcgg cctcggagga ccatccgaag tcgaccaggc cgtcgaagct
      181 gatgttcatg tacgcaccgc ccgcccgcgg agcgggggcc gatccgggcg acgccgatgc
      241 cacggacaaa ggcgtgtccc ggtcggcggc cgtcaggctg tccacccggg actggagtgt
      301 gcgcacggcc aaggccaccg aatcgagcgc ggcccgctgc cggacggaac cccgatcgga
      361 agcctgcgca tgggctggaa agggccacaa tatcgcggcc aggacgaacg gcgcgacgga
      421 cgtcgcgcgg atgccatggg tcggcatgaa tccctcgtgg ttcggacccc cggcacgcgc
      481 gcgcgtgaaa gccttgaaac gggcggttcg gggtcatggc gaccgcgtgg cgtggaagca
      541 actcttccac ggcggcgcgc gatcgtgacg aacgggatct cgggcgcggc aaccgatcgc
      601 cggatcgcgg agcgatcacg ggatcggggc gcgtctagcc gatctgggga ggggctctca
      661 ggcgatgggc gtcgcaggcc gacgcgaccg ccggggaagt cgccggaaga acgaccgcga
      721 acggggcgcc gggaaggatg acactcgcgg tgaccgacac caccggcacg gcgccggcag
      781 cttcgtccgc caggcggcag gtggggcaat cggcatgatg gccggtgtcg tggtcatggt
      841 gcgccccgac cgccacgatg ccggtggcga gcaccaggag cgacaggatg agcgccagga
      901 gcggacggtg ccggaaggtc ctgatccacg acatggcagt gacgctacac cccacgcgca
      961 agatgggtca acaatcgatc accgcgccac ttccaggacc cgctccgacc agtcgaagag
     1021 ccgctcgcac cgacgctgca gcgcgctgaa gtcgatgaca tccgtgccgg ccagcgcctt
     1081 cgcgatgtcg gcttcggagc cggccgccag cccgtcgggc gtggcgaaca ccgcctcgcg
     1141 gcggaggtcg gtatcgagaa accactcggg gccgatccgt tcgcgcaggt gttcctcgac
     1201 ggcggcggcc agcgccacgg cccgcgccgc atcgagtccc ggcaggaagt caccgcgatt
     1261 ggcaagcgcg tcgtacgact cggccggcgt caggtcgagg ccgcgcgcaa acccccggat
     1321 ctggcggatg agctctccct cgtccacgcc ggcaggggca aagacgtacg gcatccagta
     1381 gtcggtaccg gcccggtggc ggatcaggtc gatcacgagc tggccggcga tctcgctccg
     1441 gatgcggcgg aggtcgttcc agagctgcca gcggatgagc cgggccaggt gaaggtcgga
     1501 gagccgggtg acatcggtgt cgcggccgga agcccggtcg tcccgacgac gctgctcgta
     1561 ccactcgggc gaactcgtga ggagcatcga cagctcggcc gacgtggccg gcaggagctc
     1621 ggggcccgca tcgcgccact ccggcttcga ctcggccgtg ccattcttct ggcgagcctc
     1681 gccggccgcg gccagggccg cgcgtgcggc ggccatgccg gtgacgtccg acaccgccag
     1741 cttcacctcc accgggggat tcgggcaaac caccacggcc tgcacgtcgg acgcatcgtc
     1801 gacgaccgcc ggccgcccct ccatcaccag gtggggggca atcgcctgga cgacgggagt
     1861 cggacgaccc ggatcgaccg ccgccgggag cgaggtccac tcgcgggcca ggacgggggc
     1921 cgcatccacg tagcggtccc acgtgtcgga ccaggggagg tgccggaagt cggcggattt
     1981 caaggatggc ggacggcgcg ccgccacgcg ggcaggaacc accgtgaccg gcgttgccag
     2041 ctcacgaccc agaagctggt acatcgtgtc ggtggcccgg atgaaaccga acaggcccgg
     2101 gagcactctc tccggatcgg tccggcgcag tcggacggcg aactcggtat agccgccata
     2161 tcccaacgcg atcgccaggc tgtcgattgc ggcgccccgc cgaaccagga gcgggtcgat
     2221 ccggtcgtgc cacagcc
//
LOCUS       DASVOD010000220         3937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_484773, whole genome shotgun sequence.
ACCESSION   DASVOD010000220 DASVOD010000000
VERSION     DASVOD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484773"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VF720_04705"
     CDS             <1..555
                     /locus_tag="VF720_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878683.1"
                     /translation="NGSFDVFTMNTTPPGKFTKLSTTIAGGNIRSVAIGKMNNDNYPD
                     LVFGERTGNNTGRVELWTNNGSGVFSMADQVAADGPVTCVALGLIDQDLYLDIAAGNE
                     ARTVQAWFCNGSVQAQVIPSAENWADANTGGTVNAIAIGKIETSIDTPDLDVLNDIVV
                     GTAITATTGDIIIYLNPYVDVITP"
     gene            575..1864
                     /locus_tag="VF720_04710"
     CDS             575..1864
                     /locus_tag="VF720_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HEX7878684.1"
                     /translation="MNGYDKNAKSDERGNVMVGALMMLLLISAMGVSYVALSKTETGI
                     AGSGKRHVQSMFTAEAGVAEALTRMSSTDTTIYFGEDADATPTPGWGRYLVQASGNSS
                     QDPNYNDTLSDSLNNDADGSTDETSEHYAEVLSAQDSDHRLPYPWVRVKYRLNSSNQV
                     VLYGDHDLNPATDPRPNLVRGFPVLQVSAEGNQGTSQRLIQVEAVRSPFSVPKAAIYS
                     ESDDFQFNGTQFLVSGQDWDPYTDTVVPGSSEVQGIETTEDPQNIIDELHNNQLNNVE
                     GDGAEPAIGVAGTNYDIPALIAQYAPLADDEHPGGTISVGTIPDWGGINDFRIVHVVS
                     DLHITGDVSGGGLLLLEGDLTCTGQFTWYGMVINTGEIAFSGGGQGIHLFGSLMTQGG
                     VSTNTVGGNADIKYSSAMLTKLTAFNPYKVSAWTELP"
     gene            complement(2086..>3937)
                     /locus_tag="VF720_04715"
     CDS             complement(2086..>3937)
                     /locus_tag="VF720_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878685.1"
                     /translation="STVGPTVGSAVGSAVGSAVGPTRWPTLGFTRGTLAGLAAVLLLG
                     TRGHVVWAGLSGMDTLLLPAALATVAARVAIAGPGPGAAWWMAGLLGWVRPEGIPLGF
                     VALLYLWLESWRHRKTGEPMPRSLPSVERLSTNALRLSASATRALVFLGAVALLLSPG
                     LLNWALTGSPQWDSMRVKGLWSETRPDVLASLVSRLPSVWGEIALLPFDDFHHFRMDS
                     PAGGLFAVVMALGFIPGWLAALAGRFGPAGRLIGIWLPIQVALGGFSPGWDAHYFRYQ
                     IPLLVPVTILTVVGWWHATRALPGRISLLTPIALAAITLLLLTGGFREMRGLFARNGA
                     NILEQQTRVGRWIAANTREGTRVALNDAGAIAYFGERPIIDMVGLVSHGWSPASRQGS
                     GALFERLESLPVEERPELLAWYPRWFPQMAATGFPGPVLMKADLVDNTICGDRLKQVQ
                     KIDWRLAGTGDRPIQHLELIRDFGMSIVDSVDIADQASEAVHGYHWYETFRDRLREFP
                     TAAPDGGVAMDGGRWIADGESMRVRARPGQWLVLVMRTEGEHVDTLDVAVNGRPAGAI
                     ALARLAAAWSEPLLQIPDSLVVDSLLSIDIRRRPSPTGDGYGSYHYWFLQ"
BASE COUNT          742 a         1312 c         1302 g          581 t
ORIGIN      
        1 aacgggtcct ttgacgtctt cacgatgaat accacgccgc ccggcaagtt caccaagctc
       61 tccacgacga tcgccggggg caatatcagg tcggtggcga tcgggaagat gaacaacgac
      121 aactatccgg acctggtgtt cggcgagcgt accggcaaca acaccggccg ggtcgagctc
      181 tggaccaaca acggctcggg cgtcttctcc atggcggacc aggtcgccgc cgacgggccc
      241 gtcacctgcg tggctctcgg cctgatcgac caggacctgt acctcgacat cgcggctggc
      301 aacgaggccc gcaccgtcca ggcgtggttc tgcaacggct ccgtccaggc acaggtcatc
      361 cccagcgccg agaactgggc cgacgccaac accggcggca cggtcaatgc gatcgccatc
      421 ggcaagatcg agacgagcat cgacactccc gacctggacg ttctcaatga catcgtggtc
      481 gggacggcga tcaccgccac gaccggcgac atcatcatct acctgaatcc ctatgtcgat
      541 gtcatcacgc cctgattgga aggagcggtc acgtatgaac ggatatgaca agaacgccaa
      601 gtccgacgag cgcggcaacg tcatggtcgg ggcgctgatg atgctgctcc tgatctcggc
      661 gatgggcgtg tcgtacgtgg ccctgtcgaa gaccgagacc ggtatcgccg gcagcgggaa
      721 gcggcatgtc cagtcgatgt tcaccgccga ggccggggtg gccgaggcgt tgacgcggat
      781 gtcgagcacc gataccacga tctacttcgg cgaggacgcc gatgccacgc cgacgcccgg
      841 ctggggacgt tacctggtgc aggccagcgg caacagttcg caggatccga attacaacga
      901 caccctgagc gacagcctca acaatgatgc ggacggctcg acggacgaga cgagcgagca
      961 ttacgccgag gtgctcagcg cgcaggacag cgatcaccgg ctgccgtacc cgtgggtgcg
     1021 ggtgaaatac cgcctgaact cctccaacca ggtggttctg tacggcgacc atgacctgaa
     1081 tccggccacc gatccccgcc cgaacctggt ccgtggattc ccggtcctcc aggtatcggc
     1141 ggagggaaat caggggacgt cgcagcgcct catccaggtg gaagcggtcc ggtcgccatt
     1201 ttcggtgccg aaggcggcca tctattcgga aagcgacgac ttccagttca acggcacgca
     1261 gttcctggtg tccggccagg actgggatcc ctacaccgat acagtggtgc ccggttcgag
     1321 cgaggtgcag ggaatcgaga cgacggaaga cccgcagaac atcatcgatg agctccacaa
     1381 caaccagctc aacaacgtgg agggcgacgg agcggagccc gccatcggtg tcgcgggcac
     1441 caactacgac attcccgccc tgatcgccca gtacgcgccg ctggccgatg acgagcaccc
     1501 cggcggcacg atttccgtcg ggacgattcc cgactggggc ggcatcaacg acttccggat
     1561 cgtgcacgtg gtcagtgacc tccacatcac cggcgacgtg agcggcggtg ggctgcttct
     1621 actcgaaggc gatctgacct gcaccggcca gttcacctgg tacggcatgg tcatcaacac
     1681 cggcgagatc gccttctccg gcggtggcca ggggattcat ctcttcggct cgctgatgac
     1741 gcagggcggc gtcagcacca acacggtcgg cggtaacgcc gacatcaagt actccagcgc
     1801 catgctcacc aagctcaccg cgttcaaccc ctacaaggtc agtgcttgga cggagttgcc
     1861 gtagcggccg gcgatttcgt cacgcgcccc gggcggtaaa caccgttcgg ggcgcatttt
     1921 tttggtcggc gtggggggcg atagccggtt cgcgcgcgcg aatgcggatc ccgagcaacc
     1981 tctagtgcaa acagtttcca ctttcaccgg tgcgagttgg tcggacctcc actttcaccg
     2041 gtgcgcgttg gtcggaccta cactttcatc ggtgctagtt gccggctatt gcaggaacca
     2101 gtagtggtag gagccgtagc cgtccccggt gggggagggg cggcgacgga tgtcgatcga
     2161 aaggagcgag tcgacgacga ggctgtccgg gatctgcaga agaggctcgc tccaggcggc
     2221 ggcgaggcgg gccagtgcga tggcgccggc cggccggccg ttcacggcca cgtcgagggt
     2281 gtcgacgtgt tccccttcgg tccgcatgac aagcaccagc cactggccag ggcgcgcccg
     2341 gacccgcatc gattcgccat cggcgatcca ccggccgccg tccatggcca cgccgccgtc
     2401 cggggcggcg gtggggaact cacgcagccg gtcgcgaaac gtttcgtacc agtgatagcc
     2461 gtgcacggcc tctgacgcct ggtcggcgat gtccacggag tcgacgatcg acatcccgaa
     2521 gtcgcggata agctcgagat gctggatcgg ccggtcgccg gtgccggcca ggcgccagtc
     2581 gatcttctgc acctgcttca gacgatcgcc gcagatggtg ttgtcgacga gatcggcctt
     2641 catgaggact gggccgggaa agccggtggc ggccatctgg gggaaccatc gcgggtacca
     2701 tgcgaggagt tcggggcgct cctcgacagg gagtgattcg agacgctcga acaaggcccc
     2761 ggaaccctgg cggctggccg ggctccaccc gtgcgacacg aggcctacca tgtcgatgat
     2821 cggtcgctcg ccgaagtagg cgatcgcgcc cgcgtcattc aaggctacgc gtgttccttc
     2881 cctcgtgttc gccgcgatcc agcgtccaac ccgggtctgc tgctcgagga tgttggcgcc
     2941 gttccgcgcg aacaggccgc gcatctcgcg gaagccgccg gtcagcagca gcagcgtgat
     3001 ggccgcgagg gcgatcggtg tcagcaagga gatccgtccg gggagagccc gggtggcatg
     3061 ccaccacccc accacggtca gtatcgtcac cggcaccagc agcggaatct ggtagcggaa
     3121 atagtgcgcg tcccagccgg gagagaaacc gccgagcgcc acctggatgg gaagccagat
     3181 cccgatcagc cggcccgccg gcccgaatcg tcccgccagg gccgccagcc acccggggat
     3241 gaagccgagc gccatgacga ccgcgaagag cccgccagcc ggggagtcca tccggaagtg
     3301 atggaagtca tcgaagggga ggagcgcgat ttcaccccac accgacggaa gccgggaaac
     3361 gagcgacgcg agcacgtccg gacgggtttc gctccatagc cccttcacgc gcatgctgtc
     3421 ccattgcggc gaaccggtca gcgcccagtt gaggagcccg ggggagagga gaagagcgac
     3481 ggctccgagg aagacgagcg cccgggtggc gctggcggat aaccgaagcg cattggtgga
     3541 gaggcgctcc acggacggaa gcgagcgcgg catcggctcc cccgtcttcc ggtggcgcca
     3601 gctctccagc cagagataga ggagcgcgac gaacccgagc gggattccct cggggcggac
     3661 ccagcccagg agccctgcca tccaccaggc tgcgccgggc cccggcccgg cgatggccac
     3721 gcgggccgcg acagtggcca gcgccgcggg gaggagcagc gtgtccattc ccgagaggcc
     3781 ggcccagacg acgtgcccgc gcgtgccaag cagcagcacc gccgccaaac cggccaacgt
     3841 accccgagtg aacccgagcg tgggccaccg agtcggcccc accgccgacc ccaccgccga
     3901 ccccaccgcc gaccccaccg tcggccccac cgtcgac
//
LOCUS       DASVOD010000221         2627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_901590, whole genome shotgun sequence.
ACCESSION   DASVOD010000221 DASVOD010000000
VERSION     DASVOD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901590"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /gene="accC"
                     /locus_tag="VF720_04720"
     CDS             complement(<1..1220)
                     /gene="accC"
                     /locus_tag="VF720_04720"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7878686.1"
                     /translation="MFKKILIANRGEIALRVIRACRDLGIQTVAVYSEADRDSIHVRL
                     ADESVCIGPALSRDSYLNIPRIISAAEITAAEAIHPGYGFLSENPQFAEVVESCGLTF
                     IGPTSEQIRRMGDKSMARQTMVAAGVPVVPGSAGPVRTPEEALEAAEVTGYPLIIKAL
                     AGGGGRGMRVVEAEDELVDRFNTARAEAQAAFNNGDVYIERYLRQPRHVEIQLLGDRH
                     GNCVSLFERDCSVQRRHQKLIEESPSPAVDPLLRKAMGDAALVGARAINYVGAGTMEF
                     LLDSDGRFYFMEMNTRLQVEHPVTEIVTGLDLVREQIRVAAGDPLSFRQDDLELKGWA
                     IECRVNAEDVAHDFRPSPGRIDYLHFPGGPGVRVDSHIYAGYRVPPNYDSLIAKIITW
                     GDTREEALARMRRSL"
     gene            complement(1255..2370)
                     /locus_tag="VF720_04725"
     CDS             complement(1255..2370)
                     /locus_tag="VF720_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7878687.1"
                     /translation="MNIKRILERMIEERASDLHVKAGTPATIRVDGRLIALDEPVPSQ
                     KDIETVVEQILTPKQRKHFEDSKEIDFAFSIPGLSRFRSNFYQQRGTIAMAFRQVPFG
                     VPTLEDLSLPMILGDLILKPRGLVLITGTVGTGKSTSMAAMLQHVNRNIAKNIISIED
                     PIEFLHRDDKSLISQREVGLDTQSFHDGLKHILRQDPDVIVIGEIRDQETMRVAIMAA
                     DTGHLVLASLHTPDAAQTINRIISFFPLQEHEEVRFLLASNLLSVCSMRLVPRKEGTG
                     RLPAVEVMINTATIKEYIMDPQKTTGIYKAIQEGVNQYGMQTFDQALMSLFKKDGISY
                     EDALRYCTNPAEFDLRAKGIHAASDKTWEHFEGEMIR"
     gene            complement(2402..>2627)
                     /gene="accB"
                     /locus_tag="VF720_04730"
     CDS             complement(2402..>2627)
                     /gene="accB"
                     /locus_tag="VF720_04730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX7878688.1"
                     /translation="PMVGTFYRSPAPDADPYIEPGDRIESGQTLCIIEAMKLMNEIQA
                     EVRGKIVRVSVSNGQPVEFGQTLFLVDEKA"
BASE COUNT          436 a          820 c          871 g          500 t
ORIGIN      
        1 agggaacgcc gcatccgggc cagcgcctct tcccgcgtat caccccaggt gatgatcttg
       61 gcgatcagcg agtcgtagtt cggcggcacg cggtagccgg cgtagatgtg gctgtcgaca
      121 cgcaccccgg gcccgcccgg aaagtgcagg tagtcgatgc gcccgggcga tggacggaag
      181 tcgtgcgcga cgtcctccgc gttgacgcgg cactcaatgg cccatccctt gagctcgaga
      241 tcgtcctgcc ggaaggagag cgggtcgcct gcggccacgc ggatctgttc ccgcacgagg
      301 tcgagccccg tcacgatctc ggtgaccgga tgctccacct ggagtcgcgt gttcatctcc
      361 atgaagtaga agcgtccgtc cgagtcgagg agaaactcca tggtgcccgc gccgacgtag
      421 ttgatggccc gggcgccgac cagcgccgcg tcgcccattg ccttgcgcaa gagtgggtcg
      481 acggccggcg agggagactc ctcgatcagc ttctggtggc ggcgctggac ggaacagtcc
      541 cgctcgaaca ggctcacgca gttgccgtgc cggtcgccga gcagctggat ctcgacgtgc
      601 cggggctggc gcaggtagcg ctcgatgtac acgtcgccgt tgttgaaggc cgcctgggcc
      661 tcggcgcggg ccgtattgaa gcggtcgacc agttcgtcct cggcttccac cacgcgcatg
      721 ccgcgcccgc caccaccggc cagtgccttg atgatcaggg ggtatccggt gacttcggct
      781 gcctcgagcg cttcttccgg cgtccgcacg ggacccgcgc tccccggcac caccggcaca
      841 cctgccgcca ccatggtctg gcgggccatc gacttgtcgc ccatgcggcg aatctgctcc
      901 gaggtggggc cgatgaacgt gaggccgcac gattccacga cctcggcaaa ctgcggattc
      961 tcggagagga agccgtaacc gggatgaatg gcctcggccg ccgtgatctc ggcggccgag
     1021 atgatgcgcg gaatgttgag gtagctgtcg cgcgagagtg ccgggccgat gcacaccgat
     1081 tcgtcggcca ggcgcacgtg gatggaatcc cggtcggcct cgctgtacac agccaccgtc
     1141 tgaatgccga gatcgcgaca ggcgcggatg acgcgcagcg cgatctcgcc ccggttggcg
     1201 atcagtatct tcttgaacat gttcccccgg aagccggacg gcgacagcgc cgaatcagcg
     1261 gatcatctcg ccttcgaagt gctcccaggt cttgtcggag gccgcgtgga ttcccttggc
     1321 ccgcaggtcg aactcggccg ggttcgtgca gtagcggagc gcgtcctcgt acgagatgcc
     1381 gtccttcttg aagagggaca tcagcgcctg gtcgaacgtc tgcatgccgt actggttcac
     1441 gccctcctgg atggccttgt agatgccggt cgtcttctgc ggatccatga tgtattcctt
     1501 gatcgtggcc gtattgatca tgacctcgac cgccggaagc cgcccggtac cttccttgcg
     1561 gggcacgagg cgcatggagc agaccgaaag gaggttcgac gccagcagga agcgaacttc
     1621 ctcgtgctcc tgcagcggga agaacgagat gatgcggttg atggtctggg ctgcgtccgg
     1681 cgtgtggagg ctggccagga ccagatgccc ggtgtcggcg gccatgatgg ccacacgcat
     1741 cgtctcctgg tcacggattt cgccgatgac gatcacgtcg ggatcctgcc tgaggatgtg
     1801 cttcaggccg tcgtggaacg actgggtatc gagccccacc tcacgctggc tgatgaggct
     1861 cttgtcgtcg cgatggagga attcgatcgg gtcctcgatc gagatgatgt tcttcgcgat
     1921 gttccggttg acgtgctgga gcatggccgc catcgacgtg gacttgccgg tgccgacggt
     1981 gccggtgatg agaaccaggc cacggggctt caggatcagg tcgcccagga tcatcggaag
     2041 cgacaggtcc tcgagcgtgg gcacgccgaa cggcacctga cggaaggcca tggcgatggt
     2101 gccgcgctgc tggtagaagt tcgaacggaa tcgcgaaagg cccggtatcg agaaggcgaa
     2161 gtcgatctcc ttcgagtcct cgaaatgctt ccgctgcttc ggggtcagga tctgctcgac
     2221 gaccgtctcg atgtccttct ggctcggcac cggttcatcg agggcgatga gccggccgtc
     2281 aacgcggatg gtggcgggcg ttccggcctt cacatgaagg tccgaggccc gttcctcgat
     2341 catccgttcc aggattcgct tgatgttcac aggcttactc ctcggttagg gaggcgggtt
     2401 ttcaggcctt ttcgtcgacc aggaacagcg tctggccaaa ctcgaccggt tgcccgttcg
     2461 acacggacac gcgcacgatt ttcccccgca cttcggcctg gatctcgttc atcagtttca
     2521 tggcttcgat gatacacaac gtctggcccg actcgatccg gtctccgggc tcgatgtaag
     2581 gatccgcgtc gggcgccggc gaacgataaa acgtgccgac catcgga
//
LOCUS       DASVOD010000222         5458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_223595, whole genome shotgun sequence.
ACCESSION   DASVOD010000222 DASVOD010000000
VERSION     DASVOD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5458
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223595"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VF720_04735"
     CDS             complement(<1..774)
                     /locus_tag="VF720_04735"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878689.1"
                     /translation="MSRQAVVSTVHVLVAGIAALAIANSPARGAIPVNNPANFVHPGT
                     PAVASKSAGGPAATSTLSGAPGKTRVAPFGAKKPTHGVATAGVRTLKPLVRGGAATTL
                     RASAPTLDAAAAHRRAELEAMNGADLKLVLDDATGTIRFLAGPRLETDPVTATPASTD
                     PMDAAGWNARAIGFLADNATLFRLDDPAAELLPVRLESDAAGFRASRFRQAWRGREIW
                     GTDLVVRFDPAGRVIGMSGHYRGTPKGVIPAARLDEASAA"
     gene            complement(787..1269)
                     /locus_tag="VF720_04740"
     CDS             complement(787..1269)
                     /locus_tag="VF720_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878690.1"
                     /translation="MARLPLLPIGLALALLAAGCSGPGKKSDARPASGSDTATAEVPA
                     YRLICGEGGGFTGRYGGFIVEADGKVVTWQGIDVSSAPTTPIGTVSAADRGQLWRALI
                     DAGFFARELNEPGNLNRFVRVEAPADTQMWNWSFDPGADSTSRVAVWNACQEIFRRVD
                     "
     gene            1377..2675
                     /locus_tag="VF720_04745"
     CDS             1377..2675
                     /locus_tag="VF720_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7878691.1"
                     /translation="MTEPTTIPGAATELPVSTMAKGLVGSEILKIAAEIRAKIAAGQN
                     VVNLTVGDFKPAVFGIPGVLKAAIAEAYEKGETNYPPSDGVPELRQAIRQFYIDRLGL
                     DYPLGSIVAAGGVRPVIYCTYRTLLDPGDTLAYPVPSWNNNHYAWLVGAKGQPIIARR
                     ETNFLPTAVDLKPYLGGTRVLILNSPLNPAGTAYGEDELTKIVELILDENGRRAASGH
                     PPLMLLYDQVYWMLTFGTTRHITPVAIDERMRDYTVFTDGMSKAFAATGLRVGWVVGP
                     EKVMKPLSDILGHVGAWAPRPEQIACAKFLGDTANLDAYSTCLKHGIEKRLVTLHEGF
                     EAMRQDGYPVESIAPQGAIYLSARINLIGRTIGGVKLDTNEQIRQLLLEKAGMAVVPF
                     QAFGLKEETGWFRLSVGALAATDLDGLFPRLREVLAEARG"
     gene            2863..3453
                     /locus_tag="VF720_04750"
     CDS             2863..3453
                     /locus_tag="VF720_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7878692.1"
                     /translation="MTDDPSQEWHSLLALLEPIHDAARASARRMAASRPDGDDLFQEA
                     VLKAVRKLPQLRDRTRFGAWFYAVMLSVHRSRARRSFWKRLVGLESVAESGIEPASAD
                     PSIDEASWRAARLSRAFARLPAVQREAVVLHDLDGYTMENVAEMQGVTVSAVKTRVVR
                     GRARLREHYVRLGFGASERAGAQPADVEGREGMING"
     gene            3446..4045
                     /locus_tag="VF720_04755"
     CDS             3446..4045
                     /locus_tag="VF720_04755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878693.1"
                     /translation="MVEKDEVTREIHADAAALKRESDRGLPPLASLSARLEKSPEAGL
                     VWRPRAIVTVGVLAAAVLLVIGAVASERFRRLIIPTRGKSAIAIEEGVKEGLAGEGFD
                     HTSVAVERAPGSLRLEVSAGDSHGTSIEMVHELEGGGSDSTLVVELPDFHDLDHLPMP
                     ERRLAIQRRLAEQGIRATVSIVDGQLRIDADERRIEAEK"
     gene            4045..5454
                     /locus_tag="VF720_04760"
     CDS             4045..5454
                     /locus_tag="VF720_04760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7878694.1"
                     /translation="MSIQRRRAFGVMLLVSVFMTMPASESAGTQSGARAPRRERGAAI
                     EALVQSSGDAPLDEFAATQLEPGYRASFEPGALRALLSAIRREAAGFGGIEGEPVEGN
                     GLRLTFLRGDRGTAVVFRIQEAEPYLITHLELQGTNVSAPDRTAIPPITWDNLELRLA
                     EEEGAGFSGVVYATRGGKVVLHRGYGMANRDTGRKNDINTVFAIGSIPIDFTRAAILK
                     LDEMGTLHTSDLISKYFEGVPADKAAMTIDHLLNGTSGLPDFHHKAGVDADRDLAWID
                     RQTAIDRILAQPLLFAPGNGEQNSHSGWVLLATLVEIVSGKGYEAFLREHFFGPAGMT
                     RTGNHESAADFLDDEFAVGYGGESVGKLNIPKYWGRTSWLVMGSGGMESTPGDLALWH
                     AAIRERKTLGDAAAARYLEPGVFAGGDDRGFFCLYNQGGDDQVIVCCNAHAGPGDRAS
                     AVARALVMMAMPPRVSSSD"
BASE COUNT          904 a         1724 c         1935 g          895 t
ORIGIN      
        1 ggcggctgaa gcctcgtcca gccgcgccgc gggaatgacc cccttgggag tcccgcgata
       61 gtggcccgac atgccgatca cacgaccggc cggatcgaag cggacgacga ggtcggtccc
      121 ccagatttcg cggccgcgcc aggcctggcg gaagcgcgag gcccggaaac cggccgcatc
      181 cgattcgagg cggacaggga ggagttcggc ggcggggtca tccaggcgga agagtgtcgc
      241 gttgtcggcc aggaaaccga tggcgcgggc gttccagcct gcggcatcca tcgggtcggt
      301 cgacgcgggc gtggccgtca cggggtcggt ctcgaggcgc gggccggcca ggaagcggat
      361 cgttccggtc gcgtcgtcca ggaccagctt gaggtcggca ccgttcatgg cttcgagctc
      421 ggcgcggcga tgggccgcgg ccgcatcgag cgtcggagcg gacgcacgca gggtagtagc
      481 ggcgccgccc cgcacgagcg gcttcagtgt gcgcacaccg gcggtcgcga ctccgtgcgt
      541 tggcttcttg gcgccgaagg gcgccacccg ggtcttgccc ggcgccccgg agagcgtcga
      601 cgtggccgcc ggcccgccgg cggacttcga tgccacggcc ggcgtgccgg ggtggacgaa
      661 attggcgggg ttgttcacgg ggatggcgcc gcgggcgggg gagttggcga tggccagggc
      721 cgcgatgccg gcaacgagga cgtgaacggt ggaaacgacg gcctgacgag acatgatcgc
      781 tcctccctag tcaacccgcc ggaagatctc ctggcaggcg ttccacacgg cgacccggga
      841 ggtgctgtcc gcgcccggat cgaaactcca gttccacatc tgcgtgtcgg cgggagcctc
      901 cacgcgcacg aatcggttga ggttgcccgg ctcgttgagc tcgcgggcaa agaagccggc
      961 gtcgatcagc gcgcgccaga gttggccacg gtccgctgcg gatacggtgc cgatgggagt
     1021 ggtcggggcc gagctgacat cgatgccctg ccaggtgacg accttgccgt cggcctcgac
     1081 gatgaatccg ccatagcgac cggtgaatcc acccccttcg ccgcagatga gccggtaggc
     1141 gggcacttcg gcagtggcgg tatcggaacc ggaggcgggg cgcgcgtcgg actttttccc
     1201 ggggccggag caaccggccg cgagcagcgc aagcgccaga ccaatcggaa ggagggggag
     1261 ccgtgccatg gtcgatgcgc aaaataacag agtcgggcat ccttggcacg ggtcggcggg
     1321 gcgccacccc tttctgctat ccttcccgcc gccgttttca ccaccctgga gaggacatga
     1381 ccgagcccac caccattccc ggcgccgcca ccgaactccc cgtttccacc atggccaagg
     1441 ggctcgtggg ttcggagatc ctcaagatcg ccgccgagat ccgggcgaag atcgccgccg
     1501 gacagaacgt ggtgaacctc accgtgggcg atttcaagcc ggcggtgttc ggcattcccg
     1561 gcgttctcaa ggcggccatc gccgaggcct acgagaaggg ggagacgaac taccccccct
     1621 cggacggcgt gccggaactc cgccaggcca tccgccagtt ctacatcgac cggctcggcc
     1681 tcgattaccc gctgggctcg atcgtggcgg ccggcggcgt gcgtccggtc atttactgca
     1741 cgtaccgcac gctgctcgat ccgggcgaca cgctcgcgta tcccgtgccg tcctggaaca
     1801 acaaccacta tgcctggctg gtgggtgcca aggggcagcc gatcatcgcc cggcgcgaga
     1861 cgaatttcct gccgacggcg gtcgacctga agccgtacct gggcggcacg cgcgtgttga
     1921 tcctgaacag tccgctgaat ccggcgggta cggcctacgg cgaggacgag ctgacgaaga
     1981 tcgtggagct gattctcgac gagaacgggc gccgcgcggc gtcgggccat ccgccgctca
     2041 tgcttctcta cgatcaggtg tactggatgc tcaccttcgg caccacccgc cacatcacgc
     2101 cggtggccat cgacgagcgg atgcgcgact acacggtctt caccgacggc atgtcgaagg
     2161 ccttcgccgc caccggcctg cgtgtgggct gggtcgtggg gcccgaaaaa gtgatgaagc
     2221 cgctctccga catccttggc cacgtcggcg cctgggcgcc gcgtccggaa cagatcgcct
     2281 gcgcgaaatt cctcggcgac accgccaatc tcgatgccta ctcgacctgc ctgaagcacg
     2341 gcatcgagaa gcgtctcgtc acgctgcacg agggtttcga ggcgatgcgc caggacggct
     2401 atccggtcga gagcatcgca ccgcaggggg ccatctatct gtcggcccgc atcaacctga
     2461 tcggccgcac gatcggcggc gtgaagctgg acaccaacga gcagatccgc cagttgctcc
     2521 tcgagaaggc cggcatggcc gtcgtgccct tccaggcctt cgggctcaag gaggagaccg
     2581 ggtggttccg gctgtcggta ggggcgctgg cggctacgga ccttgacggg ctgttcccgc
     2641 gcctgcggga agtgctggcg gaggcgcggg ggtaggggtc cccagtctgg gcgcactttt
     2701 tgctgttacg gaatcagtcc acccaacttg gttcaaaccg agttgggtga cttcattttc
     2761 gggctgatgg cctgacacca aaaaagttgc gaccattcgc cgtcgaagcg gcgtcatcat
     2821 cctgaatcaa cgacaggccc attaccgtca cccgggcggc ccgtgaccga cgacccctca
     2881 caggagtggc actccctcct cgcgcttctc gagccgatcc acgacgctgc gcgcgcgagc
     2941 gcccggcgaa tggccgcctc gcgcccggac ggggacgacc ttttccagga agccgtgctg
     3001 aaggcggtac ggaagttgcc gcagctccgg gatcggacgc gtttcggggc atggttctac
     3061 gcggtgatgc tgtcggtgca tcggagccgg gcgcggcgat cattctggaa gcggctggtg
     3121 gggttggagt cagttgccga atcaggaatt gaacctgctt cggccgatcc atcaatcgac
     3181 gaagcctcgt ggcgcgccgc gcggctctcc cgggcgttcg cccggctgcc ggcggtccag
     3241 cgggaggccg tcgtcctgca cgacctggat ggctacacga tggagaacgt ggccgagatg
     3301 cagggggtga cggtgtcggc ggtgaagaca cgcgttgtgc gcgggcgggc gcggttgcgt
     3361 gaacactacg tgcgactcgg gttcggggcg tcggagcggg ccggcgccca gcccgccgat
     3421 gtcgaaggac gagaaggaat gatcaatggt tgagaaggac gaggtcacgc gcgagatcca
     3481 tgccgatgcg gcggcgttga agcgcgagtc ggatcgtggg ctgccaccgt tggcttcatt
     3541 gtcggcacgg ctggagaagt cgccggaagc cggcctcgtg tggcgacctc gggcgatcgt
     3601 gaccgtgggc gtgctggcgg ccgcggtcct cctggtcatc ggcgcagtgg cgtcggagcg
     3661 gttccgaagg ttgatcatcc cgacccgtgg aaagagcgcg atagccatcg aggagggtgt
     3721 gaaagaggga ctggccgggg aaggctttga tcatacgtcg gtggccgtcg agcgagcgcc
     3781 gggttcgctc cggttggagg tgtcggccgg tgactcgcac ggaacttcga tcgagatggt
     3841 tcatgagctt gaagggggag gctccgacag cactcttgtc gttgagcttc cggactttca
     3901 cgacctcgat cacctcccca tgccggagcg ccggttggcc atccagaggc gcctggctga
     3961 gcaggggatc agggccaccg tgtcgatcgt ggatggacaa ttgcggatag acgcggatga
     4021 gcgtcggatc gaggcggaga agtgatgtcg atacagcgtc ggcgggcatt cggagtcatg
     4081 ctgctggtat cggtcttcat gacgatgccg gcgtcggaga gtgccggcac ccagagcggc
     4141 gcccgcgcgc cccgtcgtga gcggggcgcc gccatcgagg cgctcgttca gagttccggt
     4201 gatgcgcccc tcgacgagtt cgcggcgacg cagctcgaac cgggctaccg ggcatcgttc
     4261 gagccgggtg cgttacgggc gttgctgtcg gccatccgca gggaggccgc cgggtttggg
     4321 ggcatcgagg gagagccggt ggagggcaac ggcctccggc tcacgttcct gcggggtgac
     4381 cggggcacag ccgtcgtctt ccggatacag gaggcggagc cgtacctcat tactcatctc
     4441 gagctgcagg ggacgaatgt atcggctccg gaccggaccg ccatcccgcc aatcacgtgg
     4501 gacaacctcg aattgcggct ggcggaggag gaaggggctg gtttttccgg cgtggtgtac
     4561 gccacgcgcg gcggcaaggt cgtcctgcac cgcggctacg gcatggccaa tcgtgacacc
     4621 ggaaggaaga acgacatcaa tactgttttc gccattggct cgatcccgat cgacttcacc
     4681 agggcggcca tcctgaaact ggatgaaatg gggacgctgc acacctccga cctgatttcg
     4741 aaatatttcg agggcgtgcc ggccgacaag gcggccatga ccatcgacca tctcctgaac
     4801 ggcacctcgg gcctgcccga cttccaccac aaggccggtg tcgatgccga tcgtgatctt
     4861 gcctggatcg accggcagac cgccatcgac cggatcctgg cgcagccact cctctttgcg
     4921 cccggaaacg gtgagcagaa ttcgcactcg ggatgggtgc tcctggccac gctcgtcgag
     4981 atcgtgtcgg gcaagggtta cgaggcgttc cttcgcgaac acttcttcgg ccccgccggg
     5041 atgacacgca ccggcaacca cgagagcgcc gccgacttcc ttgacgacga gtttgccgtc
     5101 ggctacggcg gggagtcggt cggcaagctg aacatcccga agtactgggg caggacatcg
     5161 tggctggtca tgggctcggg cggcatggaa tcgacgccag gagacctggc cctctggcac
     5221 gcggccatcc gcgaacggaa aaccctgggg gatgcggccg cggcgcgcta tctcgaaccg
     5281 ggggtctttg ccggcggcga cgatcgaggc ttcttctgtc tctacaacca ggggggcgat
     5341 gaccaggtga tcgtctgctg caacgcgcac gcgggtccgg gagatcgcgc ttcggccgtg
     5401 gcgcgagcac tggtgatgat ggccatgccg ccgcgcgtca gctcgagcga ctgatccc
//
LOCUS       DASVOD010000223         7914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_588936, whole genome shotgun sequence.
ACCESSION   DASVOD010000223 DASVOD010000000
VERSION     DASVOD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588936"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2743
                     /locus_tag="VF720_04765"
     CDS             <1..2743
                     /locus_tag="VF720_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878695.1"
                     /translation="SSAGATYTVDVISGTSAAPVYTNGATLTRTGGGWAQPSGNQLPQ
                     SAPNAGASACGATPCRIESQDAQVRSAPVFRNGSLYYTQTIGLPTGTLTRTAVQWTRL
                     TAVGGAFAEGGRIDDPTATATNGGKWYAYGSIAVNSVGDFIVGYTQFSSAQHPSAGYS
                     LRLVGDGAGTIRDPFIYKVGDDYYHKTFSTATGRNRWGDYSTSQVDPSDDRSLWTIQE
                     YAKTRTGTDDGNTGSNSSRWSSWWAGVKTAYTITASAGPNGSVSPSGATAVVVGGSQA
                     YTITANACYHIVDVLVDGVSQGPIANYNFTNVTADHTISATFALNTYTIVASAGAGGS
                     ISPSGNVNVNCGANQSFAITANACFAILDVVVDGSSVGPVANYTFNTVTANHTISASF
                     TPLSYTITATAGTGGSITPSGAVNVACGDEQVFVIEPQDKCWVIADVLVDGVSVGPVS
                     SYAFGNVQANHTIDATFTQLGPFTITATANPGGTITPAGAVLVDCGASQTFTFEPTDK
                     CWYVSQILVDGVEVDEAGFYIFTDVQANHTIEVSFAQGDPFTITATAGPGGSITPSGS
                     VSVACGDDQNFTIVANVGFVIDDVVVDGSSVGPVGVYNFTDVQEDHTIDATFRDVACP
                     YVEVLVPNGGEELIIGLNTNLTWNASDNVGVTCVDLHLSRTGSGGPFEVIASCIANSG
                     SYPWTVTGPATSDAWFKVIARDEAGNECADLSNEPFTIEDVVVPILLSQFQVNALENG
                     VELRWGVSDAASFIKSGIERSDRFEGPYVELTAPVRVDGMQSILVDRDVTNGQLYWYR
                     LAGLTRSGQVVTFEPLSVTAGLPISAFSLPAIKPNPTRGVAHVTFSVPAPSHVKLTVF
                     DIQGRAIATLADRSFDPGRYELSWNGEGNDGRVPAGTYFVHLESANTRLVQRVVLVH"
     gene            complement(2810..3598)
                     /locus_tag="VF720_04770"
     CDS             complement(2810..3598)
                     /locus_tag="VF720_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878696.1"
                     /translation="MMIVLGLTAIGCQRTTPTPQTATPDMEPAPAEPVEPPPPDVVGN
                     WTITGHHIPGTSAMTDAEAAAWHGRTVRLGSALIVAGEQSCSTPRYESHRVYTQPFLA
                     KAYKLAPGSLEPLSARERVVVTESYCGRKRFTGPGAIMIGLDDNHALAPWDGVFFELT
                     RGPSFRAIGQEPGWALDFRDDGTMRFEYGYGESAVTAGITRPTVDPDTKIRTWKTAAA
                     GGDLVVTVEPKPCADAMSGLPFETTVTVTYGGTTYQGCGWTMPR"
     gene            3692..4513
                     /locus_tag="VF720_04775"
     CDS             3692..4513
                     /locus_tag="VF720_04775"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7878697.1"
                     /translation="MTDLNPQARQMADESMVRTLAAQTEAIWPQEAPLLERYGLPSDI
                     RILDAGCGTGEASSRLAALFPGAQVLGVDIEESSLALARSRYEALAPRLRFENRSIYE
                     LGLPNDMFDLTVNRHVIHSIPYPERVLAELVRVTRPGGRLHFIPEDYGMIHFPRHGLD
                     TRRFWLDGPAQFGDATGTDLFSGRHIYPLLLNLGVQDIAIDYIVVDPLRVPRETFAAI
                     WTAWRDGYVEPIAEHTNLTVDEARATFNQMIDDIRDPSRYAVWMVPVVTGVVGKG"
     gene            4798..6606
                     /gene="pepF"
                     /locus_tag="VF720_04780"
     CDS             4798..6606
                     /gene="pepF"
                     /locus_tag="VF720_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEX7878698.1"
                     /translation="MSATALPPRESIPVAERWNIESVFPTEAAWESEMAALPGVLADL
                     EKFRGRLHEGPSVLKNWLETSWTARLRVERAAIYGTMGHHVDAADPVWRQRNDRGQGM
                     RAKMLEAIAFAEPEVLAIGKETIHKWIDGTAALHVYDMWANRLFDRQPHIRSSDVEEV
                     LGALADPFTGASRIHGSASDTDLKFQPAKGSDGRTIAIGQGNIGRLLSDGDAALRRSA
                     WENYADGYLSLKNTFADCLVTGAKQNVFIARTRRYGSALEASLAPREVPNAVFHNLIA
                     AYRKNLPTWHRYWGIKKRALKLDQFRPCDVAAPIAGKRPELGWKQSVDWIADGMAPLG
                     AEYVKAFTRGINEQRWVDWRPNQGKRGGAYSGGYAGTHPFIFMSFNNDVFGLSTLAHE
                     IGHSMHSYLAWETQPPVYSDYTDFVAEVASNFNQALVRKHLLETQKDPAMRLAILEEA
                     MSNLHRYLFLMPALERLEYEVHTRVEQDKGVSADDLIGLMADYFGEAYGPEVALDRDR
                     AGITWAQFPVHMYLNYYVFTYATGIAAAHALAARVLAGEAGAREAYLEFLKSGGSLPP
                     MEALKRAGVDMTKPEPVDKAFEVLAGYVDEVGKLVG"
     gene            complement(6573..6806)
                     /locus_tag="VF720_04785"
     CDS             complement(6573..6806)
                     /locus_tag="VF720_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX7878699.1"
                     /translation="MVNHLKAFRADRNWSQDQLASRVGVSRQTIHAIERGKSEPSLSL
                     AFRLARLFDVTIEEIFAPEARSDHPTSFPTSST"
     gene            7055..>7914
                     /locus_tag="VF720_04790"
     CDS             7055..>7914
                     /locus_tag="VF720_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878700.1"
                     /translation="MKSGRSGAPASKPVGVRRLGVVWLGVLSCSMAVPMSAPRARAAA
                     FDVPGTHVTLQLAVAAAAVSADVDNVITISASPVSTTTTVDIGAAFGPARHLLIRPAP
                     ALPRASLVNTAPSVPILALASAANVTIQDLDILRNITNNEDVVSIDVCDDIVIERCRI
                     GSNWTTPGTAGWACVRMTYPTRVLLRNNICFARSPGTFDYGIHAGSFNDPANELRLYN
                     NLVSDYKVYGIRIEGVIGGALVLLRNNVAVNYTNLNPEPIAYRSEVLLGGPTVVTSHN
                     TSFASAGFVQ"
BASE COUNT         1319 a         2728 c         2596 g         1271 t
ORIGIN      
        1 gagctcggcc ggcgccacgt atacggtgga cgtcatcagc ggcacttcgg ccgcaccggt
       61 gtacaccaac ggcgccaccc tcacgcgcac cggcggcggt tgggcgcagc cgagcggcaa
      121 ccagttgccg cagtccgcgc cgaacgcggg cgccagcgcc tgcggcgcca cgccgtgccg
      181 catcgaatcg caggacgccc aggtgcgttc cgcacccgtg ttccgcaacg gctccctcta
      241 ctacacgcag accatcggct tgccgacggg cacgctcacc cgcaccgccg tgcagtggac
      301 gaggttgacc gccgtcggcg gtgccttcgc ggaaggtggc cgaatcgacg atccgaccgc
      361 cacggccacc aacggtggca agtggtacgc ctacggcagc atcgccgtga actcggtggg
      421 cgacttcatc gtcggctaca cccagttctc gtcggcgcag catccgtcgg ccggctattc
      481 gttgcggctg gtgggtgacg gcgcgggcac catccgcgat ccgttcatct acaaggtggg
      541 cgacgactac taccacaaga ccttcagcac ggcgacgggc cgcaaccgct ggggcgatta
      601 cagcacctcc caggtggatc cctcggacga ccgctcgctc tggacgatcc aggagtacgc
      661 caagacccgc accggcaccg acgacggcaa caccggctcg aactcgagcc gctggagcag
      721 ctggtgggcc ggcgtgaaga cggcgtacac catcaccgca tcggccgggc cgaacgggtc
      781 ggtatcgccc tcgggcgcca cggccgtggt tgtcggcggc agccaggcgt acacgatcac
      841 ggcgaacgcc tgctaccaca tcgtcgacgt gctggttgac ggcgtgtcgc agggaccgat
      901 cgccaactac aacttcacca acgtgaccgc cgaccacacg atcagcgcca cgttcgccct
      961 caacacctac acgatcgtgg cctcggccgg cgcggggggc tccatcagcc cgagcggcaa
     1021 tgtcaacgtg aactgcggcg ccaaccagtc gttcgccatc accgccaacg cctgcttcgc
     1081 catcctcgac gtcgtggtcg acggttcgtc ggtcggcccg gtggccaact acacgttcaa
     1141 taccgtcacg gccaaccaca ccatctcggc cagcttcacg ccgctcagct acacgatcac
     1201 ggccacggcc ggcacgggcg gcagcatcac gccttccggc gccgtcaacg tggcgtgcgg
     1261 cgacgaacag gtcttcgtca tcgagccgca ggacaagtgc tgggtcatcg ccgacgtcct
     1321 ggtggacggc gtgtcggtcg gaccggtgtc gtcctacgcg ttcggcaacg ttcaggccaa
     1381 ccacacgatc gatgccacct tcacgcagct cgggcccttc accatcacgg ccacggccaa
     1441 cccgggcggc acgatcacgc cggccggcgc cgttctcgtg gattgcgggg ccagccagac
     1501 cttcaccttc gagccgaccg acaagtgctg gtacgtctcc cagatcctgg tggacggcgt
     1561 cgaggtggac gaggccgggt tctacatctt caccgacgtc caggccaacc acaccatcga
     1621 agtgtcgttc gcccagggcg atccgttcac catcacggcc acggccggcc ccggcggcag
     1681 catcaccccg tcgggttccg tttcggtggc ctgcggtgac gaccagaact tcaccatcgt
     1741 ggccaacgtc ggcttcgtca tcgacgacgt ggtcgtcgac ggcagctcgg tcggcccggt
     1801 gggcgtgtac aacttcaccg acgtccagga agaccacacg atcgacgcca cgttccgcga
     1861 cgtggcctgc ccgtacgtgg aggtcctcgt gccgaacggc ggcgaggagc tcatcatcgg
     1921 gctcaacacc aacctgacgt ggaacgcgtc cgacaacgtg ggcgtcacct gcgtcgatct
     1981 ccacctctcc cgcacgggtt ccggtggtcc gttcgaagtc atcgcgtcgt gcatcgccaa
     2041 cagcggaagc tatccctgga cggtgaccgg tccggcaacg agcgacgcct ggttcaaggt
     2101 gatcgcgcgt gacgaggccg gcaacgagtg cgcggacctc agcaatgagc cgttcacgat
     2161 cgaagacgtc gtcgtgccga tcctcctctc gcagttccag gtgaatgccc tggagaacgg
     2221 cgtggagctt cggtggggtg tgagcgatgc cgcctcgttc atcaagagcg gcatcgagcg
     2281 ctccgaccgg ttcgaaggac cgtacgtgga gctgacggcg ccggtgcgcg tggacggcat
     2341 gcagtcgatc ctggtggacc gtgacgtgac gaacggacag ctctactggt accggctggc
     2401 gggcctcacc cgctccggcc aggtggtcac gttcgaaccg ctctcggtca ccgccggcct
     2461 gccgatcagc gcgttcagcc tgccggccat caagccgaac ccgactcgcg gcgtggcgca
     2521 cgtgaccttc tcggtgccgg cgccgtcgca cgtgaagctc acggtgttcg acatccaggg
     2581 acgtgccatc gccacgctgg ccgaccgctc gttcgacccg ggtcgttacg agctctcgtg
     2641 gaacggggaa gggaacgacg gccgcgtgcc ggcgggaacc tacttcgtcc acctggagtc
     2701 ggccaacacg cggctcgtgc agcgcgtggt gctcgtccac taggacgttc gttcgattga
     2761 accaggggat cgtccgggcc tttccggccg gacggtcccc tttctttttc tagcggggca
     2821 tcgtccagcc gcacccctgg taagtggtgc cgccgtaggt cacggtcacg gtcgtctcga
     2881 acggcaggcc gctcatcgcg tcggcgcacg gcttcggctc gaccgtcacg accaggtcgc
     2941 cgccggcggc ggcggtcttc caggtgcgga tcttcgtgtc gggatcgacc gtcggccgcg
     3001 tgatgccggc cgtcacggcc gactcgccat agccgtactc gaagcgcatg gtgccgtcgt
     3061 cgcggaaatc gagcgcccac cccggctcct ggccgatggc gcggaacgac ggaccgcgcg
     3121 tgagctcgaa gaacacgccg tcccacgggg ccagcgcatg gttgtcgtca agtccgatca
     3181 tgatggcgcc cggtcccgta aagcgcttcc ggccgcagta cgactcggtc acgacgacgc
     3241 gttcgcgtgc gctcagcggt tcgaggctgc cgggggccag cttgtacgcc ttggccagga
     3301 acggttgggt gtacacgcgg tgcgactcgt accgcggggt ggaacacgac tgctcgcccg
     3361 ccacgatgag cgcggatccc agccgaaccg tgcgcccgtg ccacgcggcg gcctccgcgt
     3421 ccgtcatggc gctcgttccg gggatgtgat gaccggtgat cgtccagttc cccaccacat
     3481 cgggcggcgg gggctcgacc ggctcggccg gggccggctc catgtcggga gtggcggtct
     3541 gcggcgtcgg agtcgtgcgc tggcagccga tcgcggtcag gccaagtaca atcatcattc
     3601 cgattccaat ccgtctcatc gtgaggaccc tccggcggca gcataacgca cccgtcggat
     3661 gccggaggcc cacccgtcac ccggagcccg catgaccgat ctcaaccccc aggccaggca
     3721 gatggccgac gaatcgatgg tccgcacgct ggccgcgcag accgaagcca tctggccgca
     3781 ggaagcgccg cttctcgagc ggtacgggct gccgtccgac atccggatcc tcgacgccgg
     3841 ctgcggaacc ggcgaggcgt cgtcgcggct ggccgcgctc tttcccgggg cacaggtcct
     3901 cggagtcgac atcgaggagt cgagccttgc cctggcacgc tcgcgatacg aggcgctggc
     3961 gccgcggttg cgcttcgaaa accgcagcat ctacgagctg ggcctgccga acgacatgtt
     4021 cgacctgacc gtgaaccgcc acgtcatcca ctccatcccg tatccggaac gcgtgctggc
     4081 cgagctcgtg cgcgtcaccc ggccgggcgg tcgcctgcat ttcattcccg aggactacgg
     4141 gatgatccac ttcccgcgcc acgggctcga cacgcgccgc ttctggctcg acggtcccgc
     4201 gcagttcggc gacgccaccg gcaccgacct cttttcgggc cggcacatct acccgctgct
     4261 cctcaacctc ggtgtccagg acatcgccat cgattacatc gtcgtcgacc cgctccgcgt
     4321 gccgcgcgag accttcgccg ccatctggac cgcctggcgc gacggctacg tggagccgat
     4381 cgccgagcac accaacctca ccgtcgacga ggcgcgcgcc accttcaacc agatgatcga
     4441 cgacatccgc gacccatcgc gctacgcggt ctggatggtg ccggtggtga cgggggtggt
     4501 ggggaagggg tgatggcggg gcgtacgagc ggctggcggg ggggctttgc gttgtcgtca
     4561 tcgcgcgatg tcttaccttg taagacagtg gggaattgag acaaccgggg ccctcccgga
     4621 gggtttcggc cgatgcggcc gggtgttacg ctgggggcgg aataccgtcc ggaggaggta
     4681 atgcccgaag tgtttgaccg ctggaggccg ccgacgtgac ggcctgcctc aggttcgcca
     4741 gccagcactg aaccgcgaag ccatcccgat tcgaccaccc accaccgagg caatccaatg
     4801 tccgcaaccg ccctcccccc ccgcgaatcc atccccgtcg ccgagcgatg gaacatcgag
     4861 agtgtctttc cgaccgaggc ggcctgggag tcggagatgg cggcgttgcc gggcgtgctg
     4921 gccgacctgg agaaattccg gggacggttg cacgaggggc cttcggtgtt gaagaactgg
     4981 ctcgagacga gctggacggc ccggctgcgg gtggagcgcg cggcgatcta cggcacgatg
     5041 gggcatcacg tggatgcggc ggatccggtc tggcggcagc ggaatgatcg cgggcagggg
     5101 atgcgggcga agatgctcga ggcgatcgcg ttcgcggagc cggaggtcct ggcgatcggg
     5161 aaggagacga tccacaagtg gatcgacggc acggcggcgc tgcacgtgta cgacatgtgg
     5221 gccaaccgcc tcttcgaccg gcagccgcac atccgttcct ccgacgtcga ggaagtgctc
     5281 ggcgcgctgg ccgatccctt cacgggggcc agccgcatcc acgggtcggc gtcggatacc
     5341 gacctcaagt tccagccggc gaaggggagc gacggcagga cgatcgccat cgggcagggg
     5401 aacatcggtc ggctcctctc cgacggtgac gcggcgcttc gccgctcggc ctgggagaat
     5461 tacgccgacg gttatctctc gctcaagaac accttcgccg attgcctcgt gacgggcgcc
     5521 aagcagaacg tcttcatcgc gcgcacgcgg cgctacggct cggcgctcga ggccagcctg
     5581 gccccgcgcg aagtgccgaa cgccgtgttc cacaacctga ttgcggccta ccgcaagaat
     5641 ctccccacct ggcatcgcta ctggggcatc aagaagcggg cgctcaagct cgaccagttc
     5701 cgcccgtgcg acgtggcggc gccgatcgcc ggcaagcgtc ccgagcttgg ctggaagcag
     5761 tcggtggact ggatcgccga cggcatggcc ccgctcggcg ccgagtatgt gaaggccttc
     5821 acccgcggaa tcaacgagca gcgctgggtg gactggcgtc ccaaccaggg gaagcggggg
     5881 ggcgcgtact cgggcggcta tgccggcacg cacccgttca tcttcatgag cttcaacaac
     5941 gacgtgttcg gcctctccac gctggcccac gagatcggcc actcgatgca ctcgtacctg
     6001 gcgtgggaaa cgcagccgcc ggtctattcc gactacaccg acttcgttgc cgaggtggcc
     6061 agcaacttca accaggccct cgtgcgcaag cacctgctcg agacgcagaa ggatcccgcc
     6121 atgcggctgg ccatcctcga ggaggcgatg agcaacctcc atcgctacct cttcctgatg
     6181 cccgcactgg agcgcctcga gtacgaagtg cacacgcgcg tggaacagga caagggcgtg
     6241 agcgccgacg acctgatcgg cctgatggcc gactacttcg gcgaggcgta cggccccgag
     6301 gtggcgctcg atcgcgaccg cgcgggaatc acctgggcgc agttcccggt gcacatgtac
     6361 ctcaactact acgtcttcac ctacgccacc ggcatcgcgg ccgcccatgc actggccgcc
     6421 cgcgtcctgg cgggtgaagc gggcgcgcgc gaggcttatc tcgagttcct caagtcgggc
     6481 gggagcctcc cgccgatgga agcgctcaag cgggccggcg tggacatgac gaagcccgag
     6541 ccggtggaca aggcgttcga ggtgctggcg ggttacgtgg acgaggtggg gaagctggtt
     6601 gggtgatcgg atcgcgcctc cggcgcgaag atctcctcga tcgtcacgtc gaacagccgc
     6661 gccagcctga acgccagcga caggctcggc tccgacttcc cccgctcgat ggcgtggatc
     6721 gtctgccgcg agacgccgac ccgggaggcc aactggtcct gggaccagtt ccggtcggcg
     6781 cggaaggctt tgaggtggtt gaccatgggg actccgaagt caattgctca atatagtagt
     6841 atgaatgtct aatagactag acaaaaagta aagcacactt gtcattattg agatttccgg
     6901 tggccaaccg gcactgaaat agcattccac cggttcgaat cagcggcgct atgctgtcgg
     6961 tccaccctat ttcgtacggg cgcaggcggg ctcgttgatg tcgacggcgc gcctcgtccg
     7021 gacacactga aactggccgc gttcggaggc tgacatgaag agtggtcggt ccggcgcgcc
     7081 ggcgtcgaag ccggttggtg tccggaggct gggtgtcgtc tggctgggcg tcctgtcgtg
     7141 ctcgatggcc gtgcccatgt cggcgccgcg cgcccgcgcc gccgccttcg acgtccccgg
     7201 cacccacgtc acgcttcaac tggcggtggc ggccgcagcc gtcagcgcgg acgtcgacaa
     7261 cgtcatcacg atcagcgcct ctccggtcag caccaccaca acggtcgaca tcggggcggc
     7321 gttcggcccg gcacgccatc tcctgatcag gcccgccccg gcgcttccgc gggccagcct
     7381 ggtcaacacg gcgccgagcg ttcccatcct tgcgctggcg tcggccgcca acgtgacgat
     7441 ccaggacctc gacatcctcc ggaacatcac gaacaacgag gacgtcgtgt cgatcgacgt
     7501 gtgcgacgac atcgtgatcg aacgctgccg catcggctcg aactggacca cgccgggcac
     7561 ggccggctgg gcctgcgtgc ggatgaccta tcccacgcgg gtgctgctgc ggaacaacat
     7621 ctgcttcgcg cggtcgcccg gcacgttcga ctacggcatc catgccggca gcttcaacga
     7681 cccggccaat gagctgcggc tgtacaacaa cctcgtatcg gactacaagg tgtacggcat
     7741 ccgcatcgag ggcgtcatcg gcggcgcgct cgtgctgctg cgcaacaacg tggcggtcaa
     7801 ctacacgaac ctgaatcccg agccgatcgc gtaccggagc gaagttctcc tcggcgggcc
     7861 caccgtcgtc acgtcgcaca acacgtcgtt cgcctcggcg ggcttcgtgc agag
//
LOCUS       DASVOD010000224         3284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_745111, whole genome shotgun sequence.
ACCESSION   DASVOD010000224 DASVOD010000000
VERSION     DASVOD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745111"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF720_04795"
     CDS             <1..286
                     /locus_tag="VF720_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878701.1"
                     /translation="GDHARGCYCGGMNLRGLLATLILSAVLLWLRAALPASAEGDTRF
                     QRRGLLVLAVVLPVAGLLIPLVPWRVAAVAIVVSTVVAVVAVRHRLPGDG"
     gene            383..1402
                     /gene="galE"
                     /locus_tag="VF720_04800"
     CDS             383..1402
                     /gene="galE"
                     /locus_tag="VF720_04800"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX7878702.1"
                     /translation="MSTILVTGGAGYIGSHILPSLRRLGYRTLVFDSLTEGHREAVGE
                     SELVIGNLGDYGALRALIRANRPLAILHFAAVCSVAESVSEPLRYYDTNVAGTLNLVR
                     AALEAGAPRIILSSTAAVYGEPKQVPIDESHPTVPINPYGWTKLAAERILADFSHAYG
                     LRYLAFRYFNAAGADPANGLGEDHRPETHLIPLALRSALDLGDPLQVYGDDYPTPDGT
                     CIRDYIHVKDLVDAHVRGLQYLLDGGDNGVFNLGTESGHSIRQIIDAVETVTQSSVEY
                     EMAPRRPGDPAVLVASARKAREVLGWEPRHDLDAIVRTALNWMQSHPNGYRDAENETA
                     AENWP"
     gene            1399..2637
                     /locus_tag="VF720_04805"
     CDS             1399..2637
                     /locus_tag="VF720_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7878703.1"
                     /translation="MIGEVVGHFEIQSELGRGGMARVFKAWDRVLNRHVAIKVLNATP
                     GEGPFAPGDSDYARILHEARAASRLDHPNIAPVHQVFTVGQRAFLVMQLVNGPSLRQR
                     LHAGPLALDEGRRIAVAIARGLSEAHRCGVLHRDIKPENILLTDRGEVKLTDFGIARV
                     AALDTGTVTATIHGTPGYLPPEVLNGAPHDARGDVYSAGLTIYETMAGGHPYARATTA
                     ATLAAVLTEDAPPLSEVQPGIPPSLSRLVMRAMAREPAERFENASDLLASLEGESPVE
                     WIPSPSGSIRAVPRVGLKRPLWQLALIPAVILAIITAAGLMWRSSRPTAAATPVVLGQ
                     VVGPTGQPVSGALVTIDGHLFQARTSSDGRFHGEVLDGTAGEQMLLRVSHDRFITHTH
                     WIELNAASKESLEIDLVPVD"
     gene            2645..>3284
                     /locus_tag="VF720_04810"
     CDS             2645..>3284
                     /locus_tag="VF720_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878704.1"
                     /translation="MTRRVCRFAPIARHPFAILRLLSILLLALWPALPAGAAEREIEL
                     RGTLLCRVDGDARRERPIETQMLIVSPVGHEEAAEVIGEAGLFRVKVPESYIDHQVIL
                     RIHRGMEEIHSLSLFLDAARVKQVGDRRVCQLGAFPLPGSCNDHSGTAAEAVMYRDEI
                     RKGGAAEPVHAAYKPKLWQGGAVAALVGAGAAALGGGSNPPGDDLASKLLPAE"
BASE COUNT          483 a         1181 c         1104 g          516 t
ORIGIN      
        1 cggggaccac gcccggggct gctattgtgg cgggatgaac ttacgggggc tcctcgccac
       61 gctcatcctg tccgccgttc tcctgtggct ccgcgccgcg ctcccggcat cggccgaggg
      121 tgatacccga ttccagcgcc gcggcctcct ggtcctggca gtggtcctcc cggtggccgg
      181 cctcctcatt cccctcgtcc cctggcgagt ggcggccgtg gcgatcgtcg tttccacggt
      241 cgtggcggtc gtggccgtcc gccatcgcct gccgggcgac ggttgacgcc cccgcccggg
      301 tgacggttga cgccccgcgc catcccccgg acaattcgcg gctcatgaaa cccacggtca
      361 gggctgccgc cgaggcatcg cgatgagcac catcctggta actggcggtg ccggctacat
      421 cggcagtcac atcctcccct cgctccggcg cctcggttac cgcacgctgg tcttcgatag
      481 cctgaccgag gggcatcggg aggcggtcgg cgagagtgag ctggtcatcg gcaacctggg
      541 ggactacggc gcgctccggg cgctcatccg ggccaaccgg ccgctggcca tcctccactt
      601 cgcggcggtc tgctcggtgg ccgaatcggt aagcgagccg ctccgctact acgacacgaa
      661 cgtggccggc acgctcaacc tcgtgcgggc ggccctcgag gcgggggcgc cgcgcatcat
      721 cctgtcctcc accgcggcgg tatacggcga gccgaagcag gtgccgatcg acgaatcgca
      781 ccccaccgtg ccgatcaatc cctacggttg gacgaagctg gccgccgaac ggatcctggc
      841 cgatttctcc cacgcgtacg gactgcgcta cctggccttc cgctacttca acgcggcggg
      901 cgccgatccg gccaatggcc tcggcgagga tcatcgcccg gaaacgcacc tgatcccgct
      961 ggcgctccgc tcggcgctcg acctgggcga tccgctccag gtgtatggcg acgactaccc
     1021 cacccccgac ggcacctgca tccgcgacta catccacgtg aaggacctgg tggatgcgca
     1081 cgtgcgtggc ctgcagtacc tgctggacgg cggcgacaac ggtgtcttca acctcggcac
     1141 cgaatcgggg cattcgatcc gccagatcat cgacgccgtg gaaacggtca cgcagtcgtc
     1201 ggtggaatac gagatggcgc cgcgccgccc cggcgatccg gcggttctgg tggcctcggc
     1261 caggaaggcg cgcgaggtgc tggggtggga accgcggcat gatctggacg cgatcgtgcg
     1321 caccgcgctc aactggatgc agagccaccc gaacggctat cgcgacgccg agaacgagac
     1381 ggccgccgaa aactggccgt gatcggcgag gtcgtcggcc acttcgagat ccagtcggag
     1441 ctcggccggg ggggcatggc ccgcgtcttc aaggcctggg accgcgtgct caaccgccac
     1501 gtggccatca aggtattgaa cgccactccg ggcgaagggc ccttcgcgcc cggcgattcg
     1561 gactacgcgc gcatcctgca cgaagcgcgg gcggcctcgc ggctcgacca tcccaacatc
     1621 gcgccggtgc accaggtgtt cacggtggga cagcgcgcct tcctcgtgat gcagctggtg
     1681 aatggcccga gcctccggca gcggctgcac gcgggtcccc ttgcgctgga cgagggtcgg
     1741 cgcatcgccg tggccatcgc gcgcggattg tccgaggccc accggtgcgg cgtgcttcat
     1801 cgcgacatca agcccgagaa catcctcctg accgaccggg gcgaagtgaa gctcaccgat
     1861 ttcggcatcg cccgggtggc ggcgctcgac acggggacgg tcaccgccac catccacggc
     1921 acgccgggct atctcccgcc cgaagtgttg aacggtgcgc cgcacgacgc gcggggggac
     1981 gtgtactcgg cgggcctcac gatctacgag acgatggccg gggggcaccc ctacgcccgg
     2041 gccaccacgg ctgccacgct ggccgccgtt ctcaccgagg acgcgccccc gctgtcggag
     2101 gtgcagcccg gcatcccccc ctcgctctcg cggctcgtga tgcgggccat ggcgcgcgaa
     2161 ccggccgaac ggttcgaaaa cgcctcggac ctcctggcct cgctcgaggg ggaaagcccg
     2221 gtcgagtgga tcccctcgcc gtcgggatcg atccgcgccg tgccgcgggt gggcttgaag
     2281 cgtccgctct ggcaactcgc cttgattccg gccgtcatcc tcgccatcat caccgccgcc
     2341 gggctcatgt ggcgttcgtc gcggccgacc gcggcggcca cgccggtcgt cctgggacag
     2401 gttgtcggcc ccaccggcca gccggtgtcc ggcgccctgg tcaccatcga cgggcacctc
     2461 ttccaggcca ggacgtcgag cgacggccgt tttcatggcg aggtcctcga cggcaccgcc
     2521 ggggaacaga tgctgctccg ggtgtcccac gatcgcttca tcactcacac gcactggatc
     2581 gagctgaatg ctgcgtcgaa agagagtctc gaaatcgatc tggtgcccgt tgactgatgg
     2641 ggcgatgacc cgtcgcgtct gccgtttcgc acccattgcg cgtcatccgt tcgcgatcct
     2701 gcgcctcctc tcgatattgc tgcttgccct gtggccggcg ctccccgccg gagccgccga
     2761 gcgcgagatc gaactgcgcg gcacgctcct ctgccgggtg gacggcgatg cccgccggga
     2821 acgaccgatc gaaacgcaga tgctcatcgt gtcgcccgtg ggccacgagg aagcggccga
     2881 agtgatcggc gaggcgggcc ttttccgcgt gaaggtgccc gagtcgtaca tcgaccacca
     2941 ggtgatcctc cgcatccacc gcgggatgga ggaaatccac tccctctccc tctttctcga
     3001 cgcggcccgc gtgaagcagg tcggcgaccg gcgggtgtgc cagctgggcg catttccgct
     3061 accggggagc tgcaacgatc attcgggcac ggcggccgaa gcggtgatgt accgcgacga
     3121 aatccgcaag ggtggcgcgg ccgaaccggt tcacgcggcc tacaagccga agctctggca
     3181 gggtggcgcg gtggccgcgc tggttggagc cggcgcggcg gcactgggcg gtggatcgaa
     3241 ccccccgggc gacgacctgg ccagcaagct cctccccgcc gaac
//
LOCUS       DASVOD010000225         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_692778, whole genome shotgun sequence.
ACCESSION   DASVOD010000225 DASVOD010000000
VERSION     DASVOD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692778"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VF720_04815"
     CDS             <1..1722
                     /locus_tag="VF720_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878705.1"
                     /translation="PDGTETLCGPEGAFTYEWSGPGGPFPDERCIQVHLPGMYSLTIS
                     DGIGCSGSDDHTLTADVAVGATTLSDVWACTGDRVEFCVTGSGTGPITYQWSRNGTPI
                     NGATDNCLVINPVAAASIGTYSVRVTGKCGSVTRSAVLRLADVTVADIPDLFLCEGQT
                     ARWCPAVTGRGPFTWEWKHNGAPIPGADDSCLVIDGVDVGDDGFYSVTVQGFCGDPVT
                     VSARLYVGTCEVFCGLTQGAYGNAGGKWNGVSRIDMLNALITPASPLVVGVLGARSVT
                     FNDGSEACIIELLPGGGPAAALKNGLGDAVVNPLTCDTNPPLQGPRGRIRNVLLAQTI
                     TLSLNVRLDPDLLTMEICDTVIVIPALPGPDGVRGTADDLPDNDHPRFLVIPASVFTA
                     LNQLGLPHTVTGVLTLANRVLAANLPPGVTLSPSDANAAAGGINDLVDECVLFIHCDD
                     ATHASAARISQLSPLDGAPDDGSTGAGQGAFLGAVRVVGSGSAVSIGYAAPAAGRLEM
                     SIFDVLGRRVATMERIVGSAGPGVERIELGDGGGVRSGIYLVRVRLDPTDGGAGVVES
                     RKIVITR"
BASE COUNT          268 a          697 c          635 g          344 t
ORIGIN      
        1 ccggacggca cggaaaccct ctgcgggccc gaaggcgcct tcacctacga gtggagcggc
       61 cccggcggtc cgttcccgga tgagcgatgc atccaggtgc atctccccgg catgtattcg
      121 ctcaccattt ccgacggcat cggctgttcg ggcagcgacg accatacgct cacggccgac
      181 gtggccgtcg gcgccaccac gctctccgat gtgtgggcct gcaccggtga tcgcgtcgag
      241 ttctgcgtca ccggcagcgg caccgggccg atcacgtacc agtggtcccg gaacggcacc
      301 ccgatcaacg gcgccaccga taactgcctc gtgatcaatc ctgtcgcggc tgccagcatc
      361 ggcacctact cggtgcgggt gaccggcaag tgcggctcgg tgacgcgctc ggcggttctc
      421 cgcctggccg acgtgaccgt ggccgacatt cccgatctct tcctgtgcga agggcagacc
      481 gcccgctggt gccccgccgt gaccggcagg gggcccttca cctgggagtg gaagcacaat
      541 ggcgcgccga ttcccggcgc ggacgacagc tgcctggtga tcgacggcgt ggacgtcggc
      601 gacgacggat tctactcggt aaccgtgcag ggtttctgcg gcgacccggt gaccgtgtcg
      661 gcgcggctgt acgtgggcac ctgcgaggtc ttctgcggcc tcacgcaggg cgcctacggc
      721 aatgccggcg gcaagtggaa cggcgtttcg cggatcgaca tgctgaacgc cctcatcaca
      781 ccggcctctc cgctggtggt gggcgtgctc ggcgcgcgat cggtcacgtt caatgacggg
      841 agcgaggcgt gcatcatcga gcttctcccc ggcggtggtc cggccgcggc cctcaagaac
      901 ggactgggcg atgcggtcgt caatccgctc acctgtgaca ccaacccgcc gctccagggt
      961 ccgcgcgggc gcatccgcaa tgtcctcctg gcccagacga tcacgttgag cctcaacgtg
     1021 cggcttgatc ccgatcttct gaccatggag atctgcgaca cggtgatcgt gatccccgcg
     1081 cttccgggtc ccgacggtgt tcgtggcacc gcggacgacc tgcccgacaa cgatcacccc
     1141 cggttcctgg tgatcccggc gtcggtcttc accgcgttga accagctggg ccttccgcac
     1201 acggtgacgg gagtcctcac gctggccaac cgcgtgctgg cggccaacct gccgcccggc
     1261 gtgaccctct cgccctccga cgccaacgcg gcggcgggcg gcatcaacga cctggtcgac
     1321 gagtgcgttc tcttcatcca ctgcgacgac gccacgcacg cttcggccgc ccggatcagc
     1381 cagctctcgc cgctcgacgg ggcgccggat gacggttcca ccggtgcggg tcagggtgcg
     1441 ttcctcggtg ccgtccgtgt ggtcggcagc ggctcggccg tgtcgatcgg ctacgcggcc
     1501 ccggccgccg gccgcctcga gatgtcgatc ttcgacgtcc tcgggcgccg ggtggccacg
     1561 atggaacgga tcgtcggaag cgccggcccc ggcgtcgagc ggatcgagct cggtgatggc
     1621 ggcggcgtcc gctccggcat ctacctcgtc cgggtgcgcc tcgacccgac cgacggggga
     1681 gcgggtgtcg tggaaagccg gaagatcgtg attacccgct gagctccttc cggaccgtga
     1741 aattcacggc ccgatccgaa tttcggtcgc cgccgtggcc tcctccggga gtccgcggcg
     1801 gcgatttccc cttctggtgc tatgatgccg caccatacct ctccttcttc gcctgcatgt
     1861 ccctcatcca aggagaagcc gtcatgtcct tgctcaagag cgttgccccg cgtcttgcct
     1921 tgctcctctg cctcgctccc tgcg
//
LOCUS       DASVOD010000226         1934 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_640837, whole genome shotgun sequence.
ACCESSION   DASVOD010000226 DASVOD010000000
VERSION     DASVOD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1934
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640837"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(264..866)
                     /locus_tag="VF720_04820"
     CDS             complement(264..866)
                     /locus_tag="VF720_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 domain-containing protein"
                     /protein_id="HEX7878706.1"
                     /translation="MNVRRILLGFAAAALLTCTALPVRAADEKPAMDQEAQMKEWMKI
                     ASPSEGHAVFDKYVGKWNADVKSWMDPAAPPMESKGSAEFSKMFGGRYMVQSFHGSMM
                     GMPFEGHGMTGYDNFRKEYQSTWMDDMSTAMMVMRGNRKSDGTFELAGTMDDPMTGQK
                     DMKMRTVEKWVDDDHFNFSMYCTMTGQEMKMMEISYTRAK"
     gene            complement(1008..>1934)
                     /locus_tag="VF720_04825"
     CDS             complement(1008..>1934)
                     /locus_tag="VF720_04825"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEX7878707.1"
                     /translation="LSPIDFGERTRAFLRVQDGCDAGCTFCIIPRIRGRSTSLSADQA
                     VERACDLLDAGYREIILTGIHLGMYGHDRPGELPSLAGLVDAIHEAATVRGHAGGFRL
                     RLSSIEPQEIDDALIDRIAAGRLVPHLHIPLQSGSDAVLTAMNRTYRAHEYDAVLSRL
                     TARIDPIGLGADVIVGFPGETDADFQATHDFIAQRPFTYLHVFPFSPRPGTRAASLPG
                     RPSGDVVDDRSARLRALSDQKRRAFAQRFVGREVDVLVEEPAGEDGWEGWSEHYVRIH
                     LDSSAGDDLTGLLLRVPAPACSGVDEPLTKGQ"
BASE COUNT          319 a          617 c          654 g          344 t
ORIGIN      
        1 gaggagttta gcggagcttg gtaccgcgcg ccagcgcggg gtccggggtt ggccgggccc
       61 gggcgagcct cggcgcgacc gggcgccccc cgacctgtgc ggaaaacagt caacaggttc
      121 cggaacctgt tgagccgacc gggccgggtg ccccgagagg ccaggtcaaa ggccccggca
      181 cggcgtgccg gggcccttta ctggcttggc tgatgccgat cgacggccga ccggagctgg
      241 ctccgtccgg gccgtcccga gggttacttc gcccgcgtgt agctgatctc catcatcttc
      301 atttcctgcc cggtcatcgt gcagtacatc gagaagttga agtggtcgtc gtcgacccac
      361 ttctcgacgg tgcgcatctt catgtccttc tggccggtca tcgggtcgtc catcgtgccg
      421 gccagttcga acgtgccgtc ggacttccgg ttgccccgca tgaccatcat ggcggtggac
      481 atgtcgtcca tccaggtgga ctggtattcc ttgcggaagt tgtcgtagcc cgtcatgcca
      541 tgcccctcga agggcatgcc catcatcgac ccatggaacg actgcaccat gtagcggccg
      601 ccgaacatct tggaaaactc ggccgatccc ttcgattcca tcggcggcgc cgcggggtcc
      661 atccagctct tcacgtcggc attccacttt ccgacgtact tgtcgaagac ggcgtgccct
      721 tccgacggcg acgcgatctt catccactcc ttcatctggg cttcctggtc catggccggc
      781 ttctcgtcgg cggcacggac aggaagcgcc gtacacgtca ggagcgcggc ggcggcaaag
      841 cccagcagaa tgcgacgaac gttcatgatc gggaactcct ttcaaacggg ttgtggcgag
      901 cccgcgccaa acctggatca gttggacgga ggtcaggcta gtccggggag cggggggcgt
      961 caaccgttac cgaaaacagt tgggtgtatt ggtgacaggg gtgggtctca ctggcctttg
     1021 gttaacggct cgtcgacacc ggagcatgca ggtgcgggta cacgaagcag cagtccggtc
     1081 aggtcgtctc cggctgacga gtcgagatgg atccggacgt agtgttcgga ccaaccttcc
     1141 cacccatcct cccctgccgg ctcctccacc aggacatcaa cctcccgtcc gacgaaacgc
     1201 tgcgcgaagg cgcgccgctt ctgatccgac agcgcgcgca ggcgcgcgct cctgtcatcc
     1261 acgacatcgc cggaaggccg gcctggcagg gacgctgccc gcgttcccgg gcgcggcgag
     1321 aacgggaaga cgtggagata ggtaaacgga cgctgcgcga tgaaatcgtg cgtggcctgg
     1381 aagtcggcgt ccgtttcgcc cgggaagccg acgatgacgt cggcgccgag gccgatcggg
     1441 tcgatccggg cggtgaggcg gctgaggacg gcgtcgtatt cgtgggcgcg gtaggtgcgg
     1501 ttcatggcgg tgagtacggc gtccgagccg gattgcaggg gaatgtggag gtgcgggacg
     1561 agccgtccgg cggcaatccg gtcgatcagc gcgtcgtcga tctcctgcgg ttcgatgctc
     1621 gagaggcgca gtcgaaaccc accggcgtga ccccgtaccg tggcggcctc gtgaatcgca
     1681 tccaccaatc ccgccaggct cggcagttca cctggtcggt cgtgaccgta catcccgaga
     1741 tggatgccgg tcaggatgat ttcgcgatac ccggcatcga gcaggtcgca cgcccgctcg
     1801 acggcctgat ccgcgctcag gctcgtcgac ctgccgcgga tgcgcgggat gatgcagaac
     1861 gtgcagccgg cgtcacagcc gtcctggacg cggaggaacg cgcgcgtgcg ctcgccgaag
     1921 tcgatcggcg agag
//
LOCUS       DASVOD010000227         1896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_327961, whole genome shotgun sequence.
ACCESSION   DASVOD010000227 DASVOD010000000
VERSION     DASVOD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327961"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VF720_04830"
     CDS             <1..974
                     /locus_tag="VF720_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX7878708.1"
                     /translation="IAEATIKVIDRAGVSIRWDRRLAGVAAVEAVGNPIPDDTLAAIA
                     ERKVALKGPLTTPVGGGFRSVNVSIRQEFDLFANLRPARTWEGLASHWPGTDLVIVRE
                     NTEGLYSGVEHWVGRKREAAEIIGVFTKSALDRICRFAFEYARRHGRRKVTAVHKANI
                     LKITNGMFLEAARGVSAEFPDIAFDDRIIDNMCLQLVRDPRQFDVIVTTNLFGDILSD
                     LTAGLTGGLGIAPSANIGAGVSVFEAVHGSAPDIAGRGIANPTSLILSACLMLDHLGE
                     AAGAARIRAALGNVLASRDRLTADLEAGRPATTAQFVDALLERIDRG"
     gene            1069..>1896
                     /locus_tag="VF720_04835"
     CDS             1069..>1896
                     /locus_tag="VF720_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7878709.1"
                     /translation="MPAEFRNEPFTDFTQPSEKAAFEKALATVAAKKGGEHPLVIGGE
                     RFILPDKIRSVNPARKTEVLGTFQGATPELAAKAVDVALAAFATWSKVPAAKRAQYLF
                     DASAVLRERKHEFSAAMVLEVGKSWAEADADTAEAIDFMEFYGREMLRYAGEQNELVY
                     LPLGVGVVIPPWNFPLAIMVGMSTAAIVAGNTVVLKPSSDTPWIAWMFFEVMEQVGLP
                     PGVLNFVTGSGGTVGNALVGHPKVRFVSFTGSKETGLHINRLAAETAPGQIWIKRVVA
                     "
BASE COUNT          318 a          641 c          623 g          314 t
ORIGIN      
        1 aaatcgccga ggccacgatc aaggtgatcg accgggccgg cgtttcgatc cggtgggacc
       61 gccgcctggc cggcgtcgcg gccgtggagg ccgttggcaa ccccatacct gatgacacgc
      121 tggcggccat tgctgaacgc aaggtggccc tgaaggggcc gctcaccacc cccgtcggag
      181 gaggtttccg cagcgtcaac gtcagcatcc gacaggagtt cgacctcttt gccaacctga
      241 ggccggcccg gacatgggaa ggccttgcct cgcactggcc gggaaccgac ctcgtcatcg
      301 tccgcgagaa caccgagggg ctctactcgg gagtggaaca ctgggtggga aggaagcggg
      361 aggccgccga gatcatcggg gtcttcacga aatcggctct cgatcggatc tgccgctttg
      421 cgttcgaata cgcacgccgg catggccggc gcaaggtgac ggccgtccac aaggccaaca
      481 tactcaagat caccaacggg atgttcctcg aggccgcccg cggggtttcc gccgagttcc
      541 ccgacatcgc cttcgatgac cggatcatcg acaacatgtg cctgcaactc gtccgggatc
      601 cgaggcagtt cgacgtgatc gtcacgacca acctcttcgg tgacatcctc tccgatctca
      661 cggcgggcct gaccgggggg ctcggtatcg ccccgtcggc caacattgga gccggcgttt
      721 cggttttcga ggcggttcat ggctccgcac ccgacatcgc cggcaggggg atcgccaatc
      781 ccacctcgct catcctgtcg gcctgtctca tgctcgacca cctcggtgag gcggccggcg
      841 ccgcccggat ccgtgccgcc ctgggaaacg tcctggcgtc ccgcgaccgg ctgaccgccg
      901 atctcgaagc cgggagaccg gccaccaccg cgcagttcgt cgacgccctc ctcgagcgga
      961 tcgatcgggg ttgagccggc cccggaagcg ttggggggat tccggcgcgt ggtctacaat
     1021 tccgtccgct cgatgacgct ccaacctttg ccggaggccc gcctcgccat gcccgccgaa
     1081 ttcaggaacg aaccgttcac cgatttcacg cagccgtccg agaaggccgc attcgagaaa
     1141 gccctggcca ccgtcgccgc gaagaagggg ggcgaacatc ccctggtgat cgggggcgag
     1201 cgcttcatcc ttccggacaa gatccgttcg gtgaacccgg ccaggaagac cgaagtgctc
     1261 ggcacgttcc agggcgccac gccggaactg gccgccaagg cggtcgacgt cgcgctcgcg
     1321 gccttcgcca cctggtcgaa ggtgcccgcg gcgaagcggg cgcaatacct gttcgacgca
     1381 tcggccgtgc tgcgtgagcg gaagcacgag ttctcggcgg ccatggtgct cgaggtcggc
     1441 aagtcatggg ccgaggccga cgccgacacg gccgaggcga tcgacttcat ggagttctac
     1501 gggcgggaga tgctccgcta cgccggcgag cagaacgaac ttgtctacct ccccctgggc
     1561 gtcggggtgg tgatcccccc ctggaatttc ccgctggcga tcatggtcgg gatgtcgact
     1621 gcggccatcg tggccggcaa caccgtcgtg ctgaagccat cgagcgacac tccctggatc
     1681 gcctggatgt tcttcgaggt catggaacag gtggggctcc cccccggtgt cctgaacttc
     1741 gtcaccggtt cgggcgggac ggtcggcaac gcgctggtgg ggcaccccaa ggtgcgtttc
     1801 gtctccttca cggggtcgaa ggaaaccggc ctccacatca atcgcctggc cgccgaaacg
     1861 gcgccggggc agatctggat caagcgcgtc gtggcc
//
LOCUS       DASVOD010000228         2492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_16516, whole genome shotgun sequence.
ACCESSION   DASVOD010000228 DASVOD010000000
VERSION     DASVOD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16516"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="ricT"
                     /locus_tag="VF720_04840"
     CDS             complement(<1..1129)
                     /gene="ricT"
                     /locus_tag="VF720_04840"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEX7878710.1"
                     /translation="MIAATVNTDVVELSFKGRRRELYLNADHLEYRTGEYLIVQADRG
                     EDIGVVVLSSHPMTLAPVARGAASGPPATAPVPVPAAEGEGALEDPEAAGHAAAPPPQ
                     ANERPRDERGGKEGHHQREGRGNRPDVRPNQLKRIVRRATMDEIGRVVDLREKEDEAA
                     RTARSKVLEHKLDMKLVDVEYQFDGNRITFYFTAEQRVDFRDLVKDLAGIFRTRIELR
                     QIGVRDESGRLGGVGVCGRPLCCTTWIREFAPITLKMAKEQNLSLSPSKLSGVCGRLK
                     CCLRYEIEFYQQAQKTYPRLGAQLPWNNRPMQVVKVDIFKEGVWLLDDDRETHWVALT
                     DLPDIPREDRLSFKKKKSAGGQGGCGNVNAGGCGAGRGEARS"
     gene            1443..>2492
                     /locus_tag="VF720_04845"
     CDS             1443..>2492
                     /locus_tag="VF720_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878711.1"
                     /translation="MTPRRRLASLLSCLPALVFLLLAAGCSDRDGDTPPNKPPVVRLT
                     GGPANGDSAEARSVFYWHGWDADGIVATYRYALVNLVEHPEVQTATDLPASAWHDTLA
                     THASFELVSPRPTLGEVEDPQHFSTGRHLLAVQAVDDAGDSSNIDWLIVTSKNVVPVT
                     TIGYPTIPVESGVVQFRQAVEVRWTGLDPDSPDPARQPVAYEWKVVPLPPNVPAFTVN
                     VNYVVGTTPGPDYAWNRVDGSVNSVRLNLQAGLDYVFAVRAIDMAGGREDVYVKGRNA
                     LPITVSSIDLFRPYLTIRDPQIGQFTFPSDGAVWEFEAPLSKCLNFELTGDASSYGGA
                     PNGFNWCLDAPNGGED"
BASE COUNT          367 a          803 c          878 g          444 t
ORIGIN      
        1 ccgaacgcgc ctcgccacgc ccggccccgc agccacccgc gttcacattc ccgcaccccc
       61 cctgtccacc ggccgacttc ttcttcttga agctaagccg gtcttcgcgc gggatgtcgg
      121 ggaggtcggt gagagccacc cagtgggttt cgcggtcgtc gtcgaggagc cagacgcctt
      181 ccttgaagat gtcgaccttg acgacctgca tggggcggtt gttccagggg agctgggcgc
      241 cgaggcgggg gtaggttttc tgggcctgct ggtagaactc gatttcgtag cggaggcagc
      301 acttcaggcg gccgcagacg ccggagagtt tgctggggga gagcgagagg ttctgttcct
      361 tggccatctt gagcgtgatg ggggcgaact cgcggatcca ggtggtgcag cagagggggc
      421 ggccgcagac gcccacgccg ccgaggcggc cggactcgtc gcggacgccg atctggcgga
      481 gttcaatgcg ggttcggaag atgccggcca ggtctttgac caggtcgcgg aagtcgacgc
      541 gctgttcggc ggtgaagtag aacgtgatgc ggttgccgtc gaactggtac tcgacgtcga
      601 ccagcttcat gtcgagcttg tgttccagca ccttgctgcg ggcggttcgc gcggcctcgt
      661 cttctttttc gcggaggtcg accacccggc cgatctcgtc catcgtggcg cggcggacga
      721 tgcgcttgag ctggttggga cgcacgtcgg ggcggttgcc gcgtccttcg cgctggtgat
      781 ggccctcctt gccgcccctc tcgtcgcggg ggcgctcgtt ggcctgggga ggcggtgcgg
      841 cagcgtggcc ggcggcctcc gggtcttcga gggcgccctc cccctccgcg gcgggaacgg
      901 gaacgggcgc ggttgccggg ggcccggaag ccgcccccct ggccaccggc gcgagtgtca
      961 tcgggtggga ggaaagcacc accaccccga tgtcctcgcc gcggtcggcc tggacgatca
     1021 ggtattcgcc ggtgcggtac tcgaggtggt cggcattgag gtagagctcg cggcgccggc
     1081 ccttgaagga gagctcgacg acgtcggtgt tgacggttgc ggcaatcatc gtggcgaccc
     1141 cacggcggat cgaacgggcg atccacccgc tggctgtctg aaaattttca gtggtctggc
     1201 gcggggtggg gagttttgga gggccgcggc tcggcgccgg agccggccgt tgggccaggg
     1261 gtccgacagg catcgaggtt cggtaatcta ttgaagcatg gccggttacg ggcccgctca
     1321 gatcgtcaaa ctcggtccac ccacatctat cgggaacgag aggccgccga aagggcggcc
     1381 atctcgtgac ggcggcacta tagcacaggc ccccggttgt cgattgggat aggatgcccc
     1441 cgatgacgcc gcgccgccgc cttgcatcgc tcctttcctg cctcccagcc ttggtcttcc
     1501 tcctcctcgc cgccggttgt tccgaccggg atggcgacac gccgcccaac aagccccccg
     1561 tggtccgatt gaccggcggg cccgcgaacg gcgattcggc ggaggcccga tcggtcttct
     1621 actggcatgg ttgggatgcc gacgggatcg tggccaccta ccgctacgcg ctggtgaacc
     1681 tggtggaaca ccccgaggtg cagacggcca ccgatctgcc ggcctcggcg tggcatgaca
     1741 ccctggccac gcacgcctcg ttcgagctcg tgagcccacg gccgaccctg ggagaggtgg
     1801 aagacccgca gcacttctcg acggggcggc atctcctggc ggttcaggcc gtggacgacg
     1861 cgggcgactc ctcgaacatc gactggctga tcgtgaccag caagaacgtg gtgcccgtga
     1921 ccaccatcgg atacccgacg atccccgtgg agagcggggt cgtgcagttc cggcaggccg
     1981 tggaggtccg ctggaccggg ctcgatcccg acagtccgga cccggcccga cagccggtgg
     2041 cctatgaatg gaaggtggtg ccgctcccgc cgaacgttcc ggcgttcacc gtgaacgtga
     2101 actacgtggt gggaaccacg ccggggcccg actacgcctg gaaccgggtg gacggcagcg
     2161 tcaattcggt gcggctgaac ctccaggcgg gactcgatta cgtctttgcc gtgcgcgcca
     2221 tcgacatggc gggtggacgg gaggacgtgt acgtgaaggg tcggaacgcg cttcccatca
     2281 ccgtttccag catcgacctc ttccgcccct atctcaccat ccgcgatccc cagatcgggc
     2341 agttcacctt tccctcggat ggtgcggtgt gggaattcga ggcgcctctc tcgaagtgcc
     2401 tcaacttcga gctcaccggc gacgcctcga gttacggggg ggccccgaac gggttcaact
     2461 ggtgcctcga cgctccgaac ggtggcgagg at
//
LOCUS       DASVOD010000229        21032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_380287, whole genome shotgun sequence.
ACCESSION   DASVOD010000229 DASVOD010000000
VERSION     DASVOD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 21032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21032
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380287"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1681)
                     /locus_tag="VF720_04850"
     CDS             complement(<1..1681)
                     /locus_tag="VF720_04850"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878712.1"
                     /translation="MRFISPLLTIATLAFAPAARASHYNITVDGNLADLIAAVNANLG
                     PANGGFTASDPLGDTYTGPCAYVNGYDLRQSYILIDYTDAMGNITPDDMILYAGWQVE
                     GIVGDVDGDGNPNTFSIGSPGGSTGCALSDEAGIGPNESYNVLLDLDCTGGVDDVRIQ
                     IKNGVVSRVVNAVVTPLAGATFNFTGTTLELKVPNYKMLLQGLEVTTDLCDARYRLTA
                     NAEFDGPGEDFSSAFQLELLPSIDVEKTPGEQAICAGDDVTWTITVTNDGLCRLDDIT
                     VTDVLGAGLTYESSDNMPTSVNGQTVTWVFEDIGLMAGQTKVISLTASTGETCSGPSL
                     TNDVTAEGVHFSPCASPEAPPASDTDMDSATVECRDKPPCDIDGPTTASIGGSVTVTT
                     THDPTVYMLNWSVIDPDGICNITSPATMGVTSIDIEFTGAGACTVQLSVKDPVNPETC
                     ITTCTHIITANPGGFACPHTIGFWRQQCAQKSGGSTKVCKTGMENLWRCVITETDVIS
                     WKKNDGSSETTASLAALGNTALFDALCSQLQGPRPMTIRDMTEIQYLGLMLN"
     gene            complement(2024..8107)
                     /locus_tag="VF720_04855"
     CDS             complement(2024..8107)
                     /locus_tag="VF720_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878713.1"
                     /translation="MLSAPRRCLVRRFLTFSLLLAALIMPAAARAAHENITIDGDLTD
                     LISAVNNNLGPSNGGFVVSDPLGDTYTGPCAYVNGYDLRQSYILVDFKDAMGNVTPDN
                     MILYAGWDMEGLVGDVDGDGNPNTFSIGSPGGSTGCALSDEAGIGPNESYNLLLDLDC
                     TGGVDDVRIQIKNGTVSRIVNAVVTPLPSATFAFAGDKLELKIPNYKALLMDLPITTD
                     LCDARMRLTANAEFDGPGEDFSGPSQLELLPSVAVTKTPGEQAICAGEPVSWTITVTN
                     DGLCRLDDITIEDTLGEGLTYANADMPPSMVKGPIIRWEFPGIGLMPGQTKVINLTAS
                     TPPECASGTLSNDVTVEAIHLNPCLAPGTPAPTASDKASASVTCRDLPPCDIAGDQNT
                     CVGRSETYTTSIGAPYTTLWSVTSTPPGICNTVGDPAGPSITVNFTGAGVCTVSLSVT
                     DPLNPEICTKTCTYLVTVNPPPPCNIEGDDHTCFPMSEVYTTNIGPNYTKSWSVTSVP
                     AGIAAINGAANGDQVTVDYTGAGTVTVHLNVTDPNDPTDCFSVCEMGVDVDAMPPCDI
                     SGDLSLCLKDPGPTQETYTTTVGAQYDRVWSIESTPPGIASFVGGNTGGSVTVNYTGL
                     GDVTVKLLVTDPADPTDCHHECDVTVAVMPPPPCDIAGDDHTCFPMSETYTTTVGSEF
                     NRTWSVMSVPPGIASINGPANGSSVQIDYTGAGTVTVKLGVFDPEDATDCVSMCEKVV
                     DVDAMPPCDISGDLHTCFPMSETYTTTVGAQYDRTWSVTSVPPGIAAINGSNTGGSVT
                     VDYTGAGTVTVHLVVTDPADPTDCHHECTVEVDVDAMPPCDIAGDLAICLDAAGAAQG
                     VYTTTVGAQYTRVWSIESTPPGIASFNGGNTGGSVTIDYTGEGSVSLKLTVTDPADPE
                     DCHHECSQTIEVTPAPPCNIDGDDHTCFPMSETYTTNVGAQYTRQWSVSSVPPGIASI
                     NGPSTGGSVLIDFTGEGTVTVKLSVFDPSDPEDCMSMCTKIVDVDAMPPCDISGDLHT
                     CFPMSETYSTTVTAAYNRAWSVSSVPPGIASINGPANGSSITVDYTGAGTVTVHLLVT
                     DPADPTDCHHECTVEVDVDAMPPCDISGDLSTCIKVPGPTQETYTTTAGAQYNRVWSI
                     ESNPPGIASFVGGVNTGGSVTLDYTGVGDVTLKLAVTDPADPADCHHECQVDIDILPT
                     PPCDIAGDDHTCFPMSEVYTTTVGAEYSRQWTVMSNPPGIASINGSATGGSVTVDFTG
                     EGQVTVKLGVFDPADPTDCLSMCEKVVDVDAMPPCDISGDLHTCFPMSETYTTTVTSA
                     YNRAWSVSSVPAGIASIVGPADGGSVTVNYTGAGTVTIKLVVTDPADPTDCVHECTAT
                     VDVDAQPPCDISGDLSTCLRVPGPTQETYTTTVGAEFSRVWSIESVPAGIASFVGGVN
                     TGGSVTLDYTATGTVTLKLTVTDPADAEDCVHVCEQTITVMPTPPCDITGDDHTCFPM
                     SETYSTTVGAGYTKAWTVMSVPPGIAAISGPANGSSVTVNFTGAGTVTVKLGVFDPND
                     PTDCLSMCEKVVDVDPGPPCDITGDLHTCFPATETYTTTVGAEYARQWSVSSVPPGIA
                     SINGSSTGGSVSIHYTGAGTVTVKLVVTDPADPIDCVHECTQTVTVEAQPSCNITGPG
                     TICADSTATFSATGGAGLTLSWSATSNPPGIFMFAGGQVGNMVHLNFSGPGTVTLTLN
                     VSFTNDPDDCHSTCMTTLTVEDCIPEPELCWMTGGGCLDEPVKKSHKKSSFGGNIHAP
                     PQSGTWEHIQRDGGNNILFNFHSWDAMVDSCWHDGGDGPCSPRTDVNNIAWSGTGKYS
                     IGNGSREYAGTFTAHVADHGEPGSHNGPCGSTDFYEITVFDTNGKVVFHAGDFLSCGN
                     MQLHPLNGNQGQAPEDWRTLVDWPEGWFNESQLLATRPYPNPVTSGATSISWSIPSGT
                     ETSRVGITVFDVAGRVIRTFDLGEQPSGDHSVDWDLRDENGTDVPSGIYFYRLAVGSE
                     AITSKLMVVRQ"
     gene            complement(8308..11952)
                     /locus_tag="VF720_04860"
     CDS             complement(8308..11952)
                     /locus_tag="VF720_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878714.1"
                     /translation="MLTATRRCTSHRLFGILLLLAALILPTAARAAHETITIDGDLTD
                     LIAAVNNNLGPANGGFTVSDPLGDTYTGPCAYVNGYDLRQGYILVDYKDAMGNETPDN
                     VILYAGWDVEGLVGDVDGDGNPNTFSIGSPGGSTGCALSDEAGIGPNESYNLLLDLDC
                     TGGVDDVRIQIKNGTVSRVVGAVVTPLAGATFAFANDKLELKITNYKALIMDLPVTTD
                     LCDARFRLTANAEFDGPGEDFSSPMQLELVPSVDVTKTPPQQEICAGEPVTWTITVTN
                     DGLCRLDDVTVTDILGVGLNYASSDNTPTNIDGQTITWVFSGLGLMPGQTKVISLTAN
                     TPAECADATLTNNVSVDAIHLNPCLDPGDSPPTATDNASATVSCRELPPCDIAGDQNT
                     CVGRSETYTTSIGAPYTTLWSVTSTPPGICNSVGDPTGSSIQVNFTGAGVCTVSLTVT
                     DPLNPELCTKTCTYLVTVNPQPPCDIDGDDHTCAPSSLVYTTTVGAEYTRAWSVSSVP
                     PGIASINGAANGGSITVDFTGAGTVTVRLLVTVPGDAEDCFHECTFPVDVDALPPCDI
                     SGDLSTCIKVPGPTQETYTTTVGAAYGRNWSVTSVPPGIATFVGGVNTGGSVTLAFSG
                     TGDVNLNLNVTDPADPEVCAHECDIDIDVLPTPPCNIDGDQNTCYPGSEVYTTTVGAE
                     YTRQWSVMSVPPGIASINGSSTGGSVTVDFTGEGTVTVKLGVFDPSDPTDCLSMCELP
                     VEVGTEPPCNITACPRQDLITTSCIGDTLYFCTPNYPAYDVQWGLTGTPMGACVIVGA
                     SNTGSVVVALAAAGTCTLTLTVTDPADPTECPATCELILTSNPLPPCNVDGPTEAAID
                     DTVTVTTTLNPADYMLMWMVEDDPDGTCEILGSTTGSSVDIHMLGSGTCTVTLRVKDL
                     NNPDECYSECGLEIEVAPEGFACPHTIGFWRAQCAQKGNGSTKVCKTGMENLWRCVIT
                     ETDVITWKKNDGSFETTASLAALGNSALFDRLCTQLQGPRPMTIRDMAELQYLGLMLN
                     VCSGALPLDIQIDSPNGDTVQEAIDFIENALNSGNGIGDAASLADDLNNRVGVLAEDC
                     EDSNSLFRNLPGCTADAAGQIGLGSNFGDPDLLATRPFPNPVTSNAVSIEYAIPNRVS
                     SADVRITIFDVSGRAVRTLLPGTQNAGSHSADWDLRDEDGTSVPSGIYFYRLTAGDET
                     ITEKLMVVRK"
     gene            complement(12285..14558)
                     /locus_tag="VF720_04865"
     CDS             complement(12285..14558)
                     /locus_tag="VF720_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7878715.1"
                     /translation="MPLNLVVFTILALLLFPASLLAGSLRASINDPRVSVHLRSALSA
                     AAAVENVATELQTNSVRPEMEIVSGGPDYHWATPDSRVLLVVEGNVDLSQLSSLNGEV
                     NTSAGGVTTLLAPLSSVPDILALDGIRGLEMEQPVELCTDISTLATNAKALWGGPPPN
                     YAATGQTGENVVIGIVDTGLDVTHQDLRTSAGSRVKFGWDQTITTGQKPAGFTYGSEY
                     TQAQINAGSYPGLDEVGHGTHITGVAAGNGRATGGGVPQFTYTGMAPEADIVFVKLMA
                     NPNGTYTDARIIDGVNYVFQKAAALNKPAVVLLAVNKMTGPHDGSDPMDIALTALTGP
                     GKILVTAAGNEYGRARHAEWTASTSQPAGSMTVNVPTYAASSNASDHFLVEAWYDAGE
                     NATVSVTTPAGLIVGPVLRGTQLTLNTPSGVVNIKNGVTTSANGSYKVEFNITRGSLT
                     YPAVAAGTWSLNYLAVTNPYFRVDAWLTSWILGSGAATFVNGKTESRLIGSPSTAQGA
                     ISVSSYTTKRTWTDISGAARSYYYAVMNQVANYASSGPRRDGALVPHITAPGYGVAGA
                     LSNTALSVVSSAYRLQDGVHYVNSGTSVAAAHVAGGVALLLQQARNLTHDQIVNQIQT
                     SATVDSYTGQVPNPRWGAGKLYLQPLAAAAVGDQAFTSQIGFRVSPNPSRGPAIFTFN
                     VPLDAASSRSMAAELVIYDVSGREVSRVARTVTGGPTEFVWDGMTNGGDEAAAGMYWG
                     RLNLDGQHATARIVRTK"
     gene            15090..17027
                     /locus_tag="VF720_04870"
     CDS             15090..17027
                     /locus_tag="VF720_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HEX7878716.1"
                     /translation="MFLTRGSPVVLLLALLGAACLMGAAAGAAETPPDSTLETAIRAL
                     VDQGLENVSMARLTDGGLDVRYENRIERWDLPAIGRVAEVCRRAVDGERSLAVTPLVR
                     GVPVRTLTATADDWGRFLAGLTSAAAFRERLAITSPARRGPATPRTNSSHGRIDLALR
                     PLLDIQLGIADDPFQTAVAVAPELTMSPLRGFLLTLQARLELQDDLDDFNNPLEPGRN
                     TLSWNGRLPAGLEVATSAGTFPENRYGVVAEMGRYLEPGGRLEARIGGDYSGFYKFLD
                     GGTISYSDAGAWSGIGTLIGRIPDREIELAVAGGHFLTGDTGVRVDLLRRFGEVELGF
                     FGVSTDEGEVGGFRFSVPLPVRHWSRPSSVRLVTVPSFPFEYREDVTPIGRRVRAYDT
                     LDRFRRGVTPVGIATNIEAIRGRSVTARFQARGLPGEPVAESGPALGGTTGLIDIPVP
                     YVTPEGTLHVGYSHIPEKWAYEGRPTLKNDYWYLTVGMLPWMETSIRATVLPGEYLID
                     DVPVDAVDRMGSLRIQSPWPRGRTTLGAGIDDIRGTRRFHSLYLVGAQSFEPHFTSMG
                     LEVVAGYGLRSLSAPRYLLDGAFGGVRLRLTPWAFAMAEHDSEKWNGGLRVVAFSRLS
                     AQLAMLNFESLSAGASWTVRF"
     gene            17069..17431
                     /locus_tag="VF720_04875"
     CDS             17069..17431
                     /locus_tag="VF720_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878717.1"
                     /translation="MTLRNKKWQWPATAALAITLLTLSGSQCSQVADRATGPDTGSLG
                     NNNDVAACVQACNETAKQDRDAEKDLFKANKEACNGDPACLDAEEARHEARMEEIAAA
                     SQLCKAPCHEQGGGGGGQ"
     gene            complement(17563..18984)
                     /gene="pyk"
                     /locus_tag="VF720_04880"
     CDS             complement(17563..18984)
                     /gene="pyk"
                     /locus_tag="VF720_04880"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX7878718.1"
                     /translation="MRRAKIVATLGPATREPKMIRRLIEAGMNVCRLNFSHGTHDEHQ
                     RTVESIHREARRLGATVAILQDLQGPKIRTGLMAAGEVLLKPGAPFVLTTRRVPGSAA
                     IVSTTFEDLPREVTRGDQLLLADGLIQLEVAHVETTDVHTRVVDGGMLGSNKGINIPG
                     RKLSVAALTPKDREDLKLGVRLGVDWMALSFVRSADDVRLLRSMLKRLKAPDLPVVAK
                     LEKPQAIANLIEILGESDAVMVARGDLAVETSPEQVPVLQKRVIREARRRGVPVITAT
                     QMLESMIEHPRPTRAEASDVANAIYDGTDAVMLSAETAVGKYPVETVELMGRIIENVE
                     NELASNPRREAESASWWRKSVADAICEAAANAAEGLGAPVVAVFTETGNTARRVAQYR
                     PGARLIAFSSSDRIRRRMALLWGVESRPAPAVSTTDRMVEWVAGALRKEGAIHPGDRI
                     VVTAGTPVGQAGSTNFLKIHQVR"
     gene            19009..20382
                     /locus_tag="VF720_04885"
     CDS             19009..20382
                     /locus_tag="VF720_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878719.1"
                     /translation="MTPPPMLGRWMAVQLPLLGLLMTMAMARLARFPTLAGQGANLLL
                     LLVILVACFCAALFVARRLCRSDAADGRGIAGVVLSFPVLLTVGILMRLPLLAAPPQL
                     SDDIYRYAWDGRVAVAGTNPYRYAPPDPALSGLRDADWRRINNPTLPTIYPPVAQRLF
                     EVATRIAPGVTGLKCLFFLFDLSVALLLSITVARSFASRWRLLVWWWHPLAVIEWSGS
                     GHVDVAGIALLAAALALAAAPAGYARWLVTGLLVGLATQVKFLALLALPFVPGAASRK
                     SGGWVAAGFVLALLVGYLPFMAPGVNVFGSLETYAARWRASDFFFGLMIRSGTAQDQP
                     ARLTDAKLFVALLAGAIATGVALLRTRPAAAVATTIGAAVVLSPTVHPWYLAWVLPFV
                     AAEFSIPWIYATLAALLAYQAVPAWLAGHVWSESAWQKGALVLPFLALAVWELARWRA
                     GRQEMAQ"
     gene            20379..>21032
                     /locus_tag="VF720_04890"
     CDS             20379..>21032
                     /locus_tag="VF720_04890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7878720.1"
                     /translation="MSAAPSSRTGVTLVIRTLNAAPWLPELLPRLAEQRRRPDELLVV
                     DSGSTDGTVERLLAAGPAITSPEPAPGDAERARVVTIPGREFSHARSTNLGFREARGA
                     IVVMISQDALPASPEWLSQLLAPLESVRAGDGTVVAAFGRHLPRPGAFALERWQIEAD
                     YPAGGAAGVLFSNVNSATRRSAWVNEPFDESLTIAEDRVWAAGQAARGRRIVYVPEAT
                     "
BASE COUNT         3250 a         6535 c         7458 g         3789 t
ORIGIN      
        1 cgttgagcat gaggcccagg tactggattt cagtcatgtc gcggatcgtc atcgggcgcg
       61 gcccctgcag ctggctgcag agcgcgtcga agagcgccgt attgccgagg gcggccagcg
      121 aggcggttgt ctcggaggat ccgtcgttct tcttccagga aatgacgtcg gtttcggtga
      181 tcacgcaacg ccagaggttc tccatgccgg tcttgcagac cttggtggag cctccggact
      241 tctgggcaca ctgctgacgc cagaagccga tggtgtgcgg acaggcgaag ccgcccggat
      301 tggcggtgat gatgtgcgtg caggtggtga tgcaggtctc cgggttgacc gggtccttga
      361 ccgacagctg gaccgtgcag gcgccggcgc cggtgaactc gatgtcgatg gaggtgacgc
      421 ccatcgtggc cggcgaggtg atgttgcaga tgccgtccgg atcgatgacc gaccagttca
      481 gcatgtagac cgtcgggtcg tgcgtggtgg tgacggtgac cgagccgccg atgctggccg
      541 tcgtcgggcc gtcgatgtcg cagggcggct tgtcgcggca ctcgaccgtg gccgaatcca
      601 tgtcggtgtc ggatgccggc ggggcctcgg gggaggcgca gggcgagaag tgcacgccct
      661 cggccgtcac gtcgttggtg aggctcgggc ccgagcaggt ttcgcccgtg ctggccgtca
      721 gcgagatgac cttggtctgg ccggccatga ggccgatgtc ctcgaagacc caggtgacgg
      781 tctggccgtt gacgctcgtc ggcatgttgt ccgagctctc gtaggtgagc ccggcgccca
      841 ggacgtcggt gacggtgatg tcgtcgaggc ggcagaggcc gtcgttggtg acggtgatcg
      901 tccaggtgac gtcgtcaccg gcgcagatgg cctgctcgcc cggggtcttt tccacgtcga
      961 tcgaggggag cagttcgagc tggaacgcgc tcgagaagtc ttcgcccggg ccgtcgaact
     1021 cggcattggc cgtcaggcgg tagcgggcgt cgcacaggtc ggtggtgacc tcaaggccct
     1081 gaagcagcat cttgtagttc ggcaccttga gctcgagcgt ggtgccggtg aagttgaagg
     1141 tggcgcctgc aagcggcgtg accacggcat tgacgacgcg gctgaccacg ccgttcttga
     1201 tctggatgcg cacgtcgtcc acgccgccgg tgcagtcgag gtcgaggagg acgttgtacg
     1261 actcgttcgg gccgatgccg gcctcgtcgg agagggcgca gccggtggag ccgccggggc
     1321 tgccgatgct gaaggtgttc gggttgccgt cgccgtcgac gtcacccacg attccttcca
     1381 cctgccagcc ggcatacagg atcatgtcgt cgggcgtgat gttgcccatc gcatcggtgt
     1441 agtcgatcag gatgtacgac tgccgcagat catagccgtt cacgtaggcg cagggccccg
     1501 tgtaggtgtc gcccagcgga tcggaggccg tgaagccgcc gttggccggc ccgaggttgg
     1561 cattgaccgc cgcgatcagg tcggcgaggt tgccgtccac cgtgatgttg tagtgggacg
     1621 cgcgggccgc cggcgcgaag gccagcgtgg cgatagtgag aagcggtgag atgaaacgca
     1681 tggcaataca tcgtctggac atggaattac ccccctgatg tcagcgctcg gcaggcgatg
     1741 cttccccgag gaccggggcc gggcggtacc gccgacgcgg ctgacggtgt gatgtaaacg
     1801 acggaaagcc gcgcggagtg cggccagggg ggggagtttc cagcccacgg ggccggtgcg
     1861 tcaacactta agaggtatcg gaatgatttc ggtccatgga accagccctg gcggcccaat
     1921 ccgggccccg gccggcgttc accggcatgg aagtaggtac aaaagaaacg gggggcggcc
     1981 cgaaggccac cccccgtcgt gacttaccgg tacttgcggc ggcctattgc cggaccacca
     2041 tcaacttgga ggtaatggcc tccgacccga cggccagccg gtagaaatag atcccggacg
     2101 gaacgtcggt gccgttctcg tcgcggagat cccagtccac cgagtgatcg ccggacggct
     2161 gctcacccag gtcgaaggtg cggatcacgc ggcccgccac gtcgaagacg gtgatgccga
     2221 cgcgcgaggt ctccgtcccg gacggaatcg accaggagat ggaggtggcg ccggacgtga
     2281 ccgggttcgg gtacggacgg gtggccagga gctgcgattc attgaaccaa ccctccggcc
     2341 agtcgaccag ggtgcgccag tcttccggcg cctggccctg gttgccgttc agaggatgga
     2401 gctgcatgtt gccgcagctc aggaagtctc ccgcatggaa gacgaccttc ccgttggtgt
     2461 cgaacacggt gatctcgtag aagtccgtgc ttccgcaggg accgttgtga ctgcccggtt
     2521 cgccgtgatc ggccacgtgc gccgtgaacg tgccggcgta ttcgcgcgat ccgtttccga
     2581 tgctgtactt gccggtgccc gaccaggcga tgttgttcac atcggtcctg gggctgcagg
     2641 gaccatcacc gccgtcgtgc cagcacgaat cgaccatggc gtcccagctg tggaagttga
     2701 acaggatatt gttgccgccg tcacgctgga tgtgctccca cgtcccggac tggggcggcg
     2761 cgtggatatt gccgccgaac gacgacttct tgtggctctt cttgaccggt tcgtccaggc
     2821 agccgccgcc ggtcatccag cagagttccg gctcggggat gcagtcctcg acggtcaggg
     2881 tggtcatgca ggtggaatgg cagtcgtcgg ggtcgttcgt gaagctgacg ttgagcgtca
     2941 gcgtcaccgt cccgggaccg ctgaagttga gatgaaccat gttgcccacc tgtccgcccg
     3001 cgaacatgaa gatgcccggg gggttggagg tggccgacca gctgagggtg agcccggcgc
     3061 caccggtcgc gctgaacgtg gcggtcgagt cggcacagat ggtgcccggt cccgtgatgt
     3121 tgcaggacgg ctgggcctcc acggtgacgg tctgggtgca ctcgtgcaca cagtcgatcg
     3181 gatcggccgg gtcggtcacc acgagcttca cggtgacggt gccggcgccc gtgtaatgga
     3241 tggacaccga accgccggtg ctcgagccgt tgatgctggc gatgcccggc ggaaccgagg
     3301 acaccgacca ctggcgggca tactcggccc cgacggtggt ggtgtaggtc tcggtggccg
     3361 ggaagcaggt gtggagatcg cccgtgatgt cgcacggcgg acccgggtcg acgtcgacga
     3421 ccttctcgca catcgacagg cagtcggtcg gatcgttcgg atcgaacacg ccgagcttca
     3481 ccgtgacggt gcccgcgccg gtgaagttca ccgtcaccga gctgccgttg gccgggccgg
     3541 agatcgcggc gatgcccggg ggcaccgaca tgaccgtcca ggccttcgtg tagcccgcgc
     3601 ccaccgtcgt cgagtaggtc tcgctcatcg ggaagcaggt gtggtcgtca ccggtgatgt
     3661 cgcacggcgg cgtcggcatg accgtgatgg tctgctcaca gacgtgcacg caatcctcgg
     3721 cgtcggccgg gtcggtaacc gtcagcttca gcgtgacggt gccggtggcc gtgtagtcga
     3781 gcgtcaccga gccgccggta ttcacaccgc ccacgaagga ggcgatgccc gccggaaccg
     3841 actcgatcga ccagacgcgg ctgaattccg ccccgacggt cgtcgtgtac gtctcctggg
     3901 tcgggcccgg gactctgagg caggtcgaaa gatcgcccga gatgtcgcac ggcggctgcg
     3961 cgtcgacgtc gaccgtcgcc gtgcactcgt gcacgcagtc ggtcggatcg gcggggtcgg
     4021 tcacgacgag cttgatcgtg accgtgccgg caccggtgta attcaccgtc accgagccgc
     4081 cgtcggccgg gccgacgatg gacgcgatgc cggccggaac cgacgatacc gaccaggcgc
     4141 ggttgtacgc ggaggtcacg gtggtggtgt aggtctcgct catcgggaag caggtgtgga
     4201 gatcacccga gatgtcgcac ggcggcatcg cgtcgacgtc cacgaccttc tcgcacatgg
     4261 agaggcagtc ggtcggatcg gccggatcga acacgccgag cttcaccgtg acctgacctt
     4321 cgccggtgaa gtccaccgtc accgaaccgc cggtagccga accgttgatg ctggcgatgc
     4381 ccggcgggtt ggacatgacc gtccactgcc ggctgtactc ggccccgacg gtcgtggtgt
     4441 agacctcgct catcgggaag caggtgtggt cgtcaccggc gatgtcgcac ggcggtgtcg
     4501 gaaggatgtc gatgtcgacc tggcactcgt ggtgacagtc tgccggatcg gccgggtcgg
     4561 tgaccgccag cttcagcgtc acgtcgccca cgccggtgta gtcgagcgtc accgaaccgc
     4621 cggtgttcac gccgcccacg aaggaggcga tgcccggcgg gttcgactcg atcgaccaga
     4681 cccggttgta ctgggctccg gccgtcgtgg tgtacgtctc ctgggtcggg cccggcacct
     4741 tgatgcaggt ggacaggtcg cccgagatgt cgcacggcgg catggcgtcg acgtcgactt
     4801 cgacggtgca ttcgtgatgg cagtcggtcg gatcggccgg gtcggtcacc aggaggtgca
     4861 ccgtgaccgt gccggcgccg gtgtagtcaa ccgtgatcga gctgccgttc gccgggccgt
     4921 tgatggaggc gatgcccggg ggcaccgacg acaccgacca ggcccggttg taggcggcgg
     4981 tcacggtggt ggagtaggtc tcgctcatcg ggaagcaggt gtggaggtcg cccgagatgt
     5041 cgcagggcgg catggcgtcg acgtccacga tcttcgtgca catcgacatg cagtcttcgg
     5101 gatcgctggg gtcgaacacc gacagcttca cggtgaccgt gccttcaccg gtgaagtcga
     5161 tcagcaccga gccgccggtc gacggaccgt tgatcgacgc gatccccggg gggacggacg
     5221 aaaccgacca ctgccgcgtg tactgcgcgc cgacgttggt cgtgtaggtc tcgctcatcg
     5281 ggaagcaggt gtggtcgtcg ccgtcgatgt tgcagggcgg cgccggggtc acttcgatgg
     5341 tctggctgca ctcgtggtga cagtcttccg gatcggccgg atcggtcacc gtgagcttca
     5401 gcgagacgct tccctcgccg gtgtagtcga tggtgaccga gccgccggtg ttgccgccgt
     5461 tgaaggaggc gatgccgggg ggcgtcgatt cgatcgacca gacgcgggtg tactgcgcgc
     5521 cgaccgtcgt ggtgtacacg ccctgggcgg cgcccgccgc gtcaaggcag atcgccaggt
     5581 cgcccgcgat atcgcaggga ggcatggcgt cgacgtcgac ttcgacggtg cactcgtggt
     5641 ggcagtcggt cggatcggcc gggtcggtca cgacaaggtg caccgtgacg gtgccggcgc
     5701 cggtgtagtc caccgtcacc gatccgccgg tgttcgagcc gttgatggcc gcgatgcccg
     5761 ggggcaccga ggtcaccgac caggtccggt cgtactgggc cccgacggtc gtggtgtagg
     5821 tctcgctcat cgggaagcag gtgtggaggt cgcccgagat gtcgcagggg ggcatggcgt
     5881 cgacgtcaac gaccttctcg cacatcgaca cgcagtcggt ggcgtcttcc ggatcgaaca
     5941 cgccgagctt cacggtgacg gtgccggcgc cggtgtagtc gatctgcacc gagctgccgt
     6001 tggccgggcc gttgatggag gcgatgcccg gcggaaccga catgaccgac caggtccggt
     6061 tgaactcgga gcccacggtg gtcgtgtacg tctcgctcat cgggaagcag gtgtggtcgt
     6121 cgcccgcgat gtcgcagggg ggcggcggca tcaccgccac cgtcacgtcg cactcgtggt
     6181 ggcagtcggt cgggtcggcc gggtcggtga cgagcagctt gaccgtcaca tcgccgagtc
     6241 cggtgtagtt gaccgtgacc gaaccgcccg tgttgccgcc gacgaaagaa gcgatgcccg
     6301 gaggcgtcga ctcgatcgac cagacgcggt cgtactgggc gcccacggtc gtggtgtagg
     6361 tctcctgcgt cgggccggga tctttcaggc agagcgacag gtcgcccgag atgtcgcagg
     6421 gcggcatcgc gtcgacgtcg acgcccatct cgcagaccga gaagcagtcg gtcgggtcat
     6481 tcggatcggt cacgttgagg tgcacggtga cggtcccggc gccggtgtag tccaccgtca
     6541 cctggtcgcc gttggccgcg ccgttgatgg ccgcgatgcc cgcgggcacc gaggtcaccg
     6601 accacgactt ggtgtagttc ggcccgatgt tcgtcgtgta aacctcgctc atcgggaagc
     6661 aggtgtggtc gtcgccctcg atgttgcagg ggggcggcgg attcacggtc acgaggtagg
     6721 tgcaggtctt cgtgcagatt tcgggattca aggggtcggt gacggacagg ctcaccgtgc
     6781 agacgcccgc gccggtgaag ttgaccgtga tcgacggacc ggccgggtcg cccaccgtgt
     6841 tgcagatgcc cggcggggtg gacgtgaccg accagagggt cgtgtacggg gcgccgatcg
     6901 aggtggtgta ggtttcgctc cggccgacgc aggtgttctg gtcgccggcg atgtcacacg
     6961 gcggcaggtc acggcaggtg accgaggccg aagccttgtc gctggcggtt ggggccggtg
     7021 ttcccggcgc caggcagggg ttcaggtgga tggcttccac cgtgacgtcg ttgctcagcg
     7081 tgcccgaggc gcactcgggc ggcgtgctgg ccgtgaggtt gatgaccttt gtctggccgg
     7141 gcatgaggcc gatgccgggg aactcccagc ggatgatcgg acccttgacc atgctcgggg
     7201 gcatgtcggc gttggcatag gtgagccctt cgccgagggt gtcctcgatg gtgatgtcgt
     7261 ccaggcggca gaggccgtcg ttggtgacgg tgatcgtcca gctgaccggc tcgccggcgc
     7321 agatggcctg ctcgcccggc gtcttggtga cggccaccga ggggagcagt tcgagctgcg
     7381 aggggccgga gaagtcttcg cccgggccat cgaactcggc gttggccgtc agccgcatgc
     7441 gcgcgtcgca caggtcggtc gtgatcggca ggtccatcag cagcgccttg tagttcggaa
     7501 tcttgagctc gagcttgtcg cccgcgaagg cgaacgtggc gctggggagc ggcgtgacca
     7561 ccgcgttcac gatccggctg accgtgccgt tcttgatctg gatgcggacg tcgtcgacgc
     7621 cgccggtgca gtcgaggtcg agcagcaggt tgtacgactc gttcggaccg atccccgcct
     7681 cgtcggagag ggcgcatccg gtcgagccgc cggggctgcc gatgctgaat gtattggggt
     7741 tgccgtcgcc gtcgacgtca cccacgagac cttccatgtc ccagccggcg tacagaatca
     7801 tgttgtccgg cgtgacgttg cccatcgcgt ccttgaagtc gaccaggatg tacgactggc
     7861 gcaggtcata gccgttcacg taggcgcagg gtcccgtgta cgtatcgccc agggggtcgc
     7921 tcacgacgaa cccgccgttc gacggcccga gattgttgtt caccgccgaa atcaggtcgg
     7981 taaggtcgcc gtcgatcgtg atgttctcgt gggcggcccg agcggccgcc ggcatgatca
     8041 gcgcggccag cagcagggaa aaggtgagaa agcggcggac aagacatcgc ctcggtgccg
     8101 aaagcatgat gaatcctcct ggtgactcgt acacggggat gggaaaaccg gagttccccg
     8161 tcggcaggct gggcggcggg tgtccggctg gctggaacga tcggcaatgc agggagccga
     8221 ccagaatttc aggagtgcgc gaccaaacga tcaacaagat ctcgccggga cggtccctcc
     8281 ggaccgtccc ggcgatggag ccaatcgtta cttgcgaacc accatcagct tctcggtgat
     8341 cgtctcgtca ccggcggtga gccggtagaa gtagatcccc gaaggaaccg aggtgccgtc
     8401 ctcgtcgcgc agatcccagt cggccgaatg ggagccggcg ttctgggtgc ccgggaggag
     8461 ggtgcgaacc gcgcggccgg agacgtcgaa gatcgtgatg cggacgtcgg ccgagctgac
     8521 gcgattcggg atggcgtact cgatggagac ggcgttcgac gtgaccggat tcgggaacgg
     8581 acgggtggcc aggaggtccg ggtcaccgaa gttcgagccg aggccgatct ggccggcggc
     8641 gtccgcggtg cagccgggga ggttgcggaa gaggctgttg ctgtcttcgc aatcctcggc
     8701 aagcacgccg acgcggttgt tgaggtcgtc ggccagcgaa gcggcatcac cgatcccgtt
     8761 gcccgaattg agcgcgttct cgatgaagtc gatcgcttcc tgcacggtgt cgccgttggg
     8821 gctgtcgatc tgaatgtcga gcgggagagc acccgagcag acgttcagca tcaggccgag
     8881 atactgcagt tcggccatgt cgcggatcgt catcggacgc ggtccctgga gctgggtgca
     8941 caggcggtcg aagagcgccg agttgccgag agcggccagc gaggctgtgg tctcgaacga
     9001 tccgtcgttc ttcttccagg tgatcacgtc ggtttcggtg atcacgcagc gccagaggtt
     9061 ctccatgccg gtcttgcaga ccttggtgga gccgttcccc ttctgggcgc actgcgcgcg
     9121 ccagaaaccg atggtgtgcg gacaggcaaa gccttccgga gccacttcga tctcgagtcc
     9181 gcactcggag tagcactcgt ccggattgtt cagatccttg acacggagcg taacggtgca
     9241 ggtgccggag ccgagcatgt ggatgtccac cgaggagccg gtggtgctgc ccaggatctc
     9301 gcacgttcca tccggatcgt cctcgaccat ccacatgagc atgtagtcgg ccgggttgag
     9361 cgtggtggtc accgtcacgg tgtcgtcgat cgccgcttcc gtgggaccgt ccacgttgca
     9421 gggcggaagc ggattcgagg tgaggatgag ctcgcacgtg gccggacact cggtcggatc
     9481 ggccgggtcg gtcaccgtca gcgtcagcgt gcaggtaccc gccgccgcta gggccaccac
     9541 caccgaaccg gtgttgctgg caccaacgat cacgcaggcg cccatcgggg tgccggtgag
     9601 cccccactgg acgtcgtagg ccgggtagtt cggcgtgcag aagtagagcg tatcgccgat
     9661 gcacgaggtg gtgatcaggt cctgccgcgg gcacgccgtg atgttgcacg gcggctcggt
     9721 gccgacctcg accgggagct cgcacatcga gaggcaatcg gtgggatccg agggatcgaa
     9781 gacgccgagc ttaacggtga cagtgccttc accggtgaag tcgacggtca ccgaaccgcc
     9841 ggtggacgaa ccgttgatcg acgcgattcc cggcggaacc gacatgaccg accactgccg
     9901 cgtgtattcc gcgccaacgg tggtcgtgta gacctcgctg ccggggtagc aggtgttctg
     9961 gtcgccgtcg atgttgcagg gtggcgtcgg gaggacatcg atatcgatgt cgcactcgtg
    10021 cgcgcacacc tcgggatcgg ccggatcggt gacattgagg ttgaggttca cgtcgccggt
    10081 gccgctgaag gccagtgtca ccgaaccgcc cgtgttgacg ccgcccacga aggtggcgat
    10141 gcccggcgga accgaggtga ccgaccagtt gcggccgtag gcagccccga cggtcgtcgt
    10201 gtaggtctcc tgggtcgggc ccggaacctt gatgcaggtc gagaggtcac ccgagatgtc
    10261 gcacggcgga agcgcgtcga catccacggg gaacgtgcac tcgtggaagc agtcttcggc
    10321 atcgcccggg acggtgacca gcagacgaac cgtgaccgtg ccggcgccgg tgaagtcgac
    10381 cgtgatggaa ccaccattgg ccgcgccgtt gatggacgcg atgcccggcg gaaccgagct
    10441 gacggaccag gcacgcgtgt actccgcgcc gaccgtggtc gtgtagacca ggctcgacgg
    10501 ggcgcaggtg tggtcgtcac cgtcgatgtc gcacggcggc tgcggattga ccgtcaccag
    10561 gtaggtgcag gtcttcgtgc agagttcagg attcagggga tcggtgaccg tgaggctcac
    10621 cgtgcagacg ccggcgccgg tgaagttgac ctggatcgaa gagccggtcg ggtcgcccac
    10681 cgaattgcag ataccgggcg gggtcgaggt gaccgaccag agcgtggtgt acggggcgcc
    10741 gatcgaggtg gtgtaggtct cgctgcggcc gacgcaggtg ttctggtcgc cggcgatgtc
    10801 gcacggcggc agctcacggc agctgacggt ggccgaagcg ttgtcggtgg ccgtcggggg
    10861 cgaatcgccc gggtcgaggc aggggttcag gtgaatggcg tcgacgctca cgttgttggt
    10921 gagcgtggcg tcggcgcatt ccgccggcgt attggccgtc agcgagatca ccttggtctg
    10981 gccgggcatc aggccgaggc cggagaagac ccaggtgatg gtctggccgt cgatattggt
    11041 cggcgtgttg tcggagctgg cgtagttgag gcccacgccc aggatgtcgg tgaccgtgac
    11101 gtcatcgagg cggcagaggc cgtcgttggt gacggtgatc gtccaggtga ccggctcgcc
    11161 ggcgcagatt tcctgctgcg gcggggtctt ggtgacgtcg acggacggca ccagttcgag
    11221 ctgcatgggg ctcgagaaat cttcgcccgg gccgtcgaac tcggcgttgg ccgtcaggcg
    11281 gaaccgtgcg tcacacaggt cggtcgtgac cggcaggtcc atgatgagcg ccttgtagtt
    11341 cgtgatcttg agctcgagct tgtcgttcgc gaaggcgaac gtggcgccgg caagcggcgt
    11401 gactaccgcg ccgacgacgc ggctgacggt gccgttcttg atctggatgc ggacgtcgtc
    11461 cacaccgccg gtgcagtcga ggtcaagaag caggttgtac gactcgttcg ggccgatgcc
    11521 ggcctcgtcg gagagggcgc agcccgtcga tcccccgggg ctgccgatgc tgaaggtgtt
    11581 cgggttgccg tcgccgtcga catcgccgac gaggccctca acgtcccagc cggcatacag
    11641 gatcacgttg tcgggcgtct cgttgcccat cgcgtccttg tagtcgacca ggatgtagcc
    11701 ctggcggagg tcatagccgt tcacgtaggc gcacgggccg gtgtaggtgt cgccgagcgg
    11761 atcgctgacg gtgaagccgc cattggccgg gccgaggttg ttgtttaccg ccgcgatcag
    11821 gtcggtgagg tcaccgtcga tcgtgatggt ctcgtgtgcg gcccgcgcgg cggtgggcag
    11881 gatcagtgcc gcgagaagga ggaggattcc gaataggcgg tgggacgtac atcgtctcgt
    11941 tgcggtgagc atgttgtgtt tccccctgtc aggagggccg ggctgtccga gccgaaacag
    12001 atgttcgtcc tggtgcaatc cggtctgacc tgactcgtga cggaactacg gaatggcggc
    12061 cgctagcgtg gtcggcgccg ggtgggctga cgcagtaatt ttctacaggt ttaatgtagt
    12121 cgtgtcaaca ctttacgggt gtcagtggta catttcgggg ggagtcccca caggagtttc
    12181 aggggacacc tgatgcgcaa gttaccagaa attctgactc acatgtcaaa aaggccgaca
    12241 cctcccggtg ccggcctttg gctaagctga ttgaggcaat cgtcttactt ggtgcggacg
    12301 atcctcgccg tggcgtgctg tccgtccaga ttgaggcgac cccagtacat cccggcggcc
    12361 gcctcgtccc ccccgttggt catcccatcc cacacgaatt cggttggtcc tccggtaacg
    12421 gtgcgggcaa cccggctcac ttcccgaccg gaaacgtcgt agatgaccag ttcggcggcc
    12481 atcgacctgg acgaggccgc gtcgagcgga acgttgaagg tgaagatggc cgggccgcgg
    12541 ctcgggttcg gtgaaacgcg gaatccgatc tgcgaggtga aggcctggtc gcccacggcg
    12601 gcggccgcga gcggctggag atacagcttt ccggctcccc accgcggatt cggcacctgc
    12661 ccggtgtagc tgtcgacggt ggccgacgtc tggatctggt tgacgatctg gtcgtgggtg
    12721 agattccggg cctgctgcag gagaagagcc acgccgcccg ccacgtgcgc ggccgcgacg
    12781 ctggtgccac tgttgacgta gtgcacgccg tcctggagcc ggtaggcgct cgacaccacc
    12841 gaaagcgccg tgttcgagag cgcgcccgcc acgccgtatc ccggtgccgt gatatgcggc
    12901 accagggcgc cgtcacgacg cggaccgctg gaagcgtagt tggccacctg gttcatgacc
    12961 gcgtagtagt acgagcgcgc ggcaccgctg atgtcggtcc aggtacgctt ggtcgtgtac
    13021 gacgaaaccg agatggcgcc ctgcgccgtg ctcggcgagc cgatcagccg tgactccgtc
    13081 ttgccattga cgaaggtggc ggctccggag ccgaggatcc agctcgtcaa ccacgcatcc
    13141 acccggaagt agggattggt gacggccaga taattgagcg accaggtgcc ggccgcgacg
    13201 gcgggatagg tgaggctgcc gcgcgtgatg ttgaactcga ctttgtacga accgttcgcg
    13261 ctggtcgtca cgccgttctt gatgttcacc acgcccgacg gcgtgttcag cgtgagctgg
    13321 gtgccgcgga gcacggggcc cacgatcagg cccgccggcg tcgtcaccga cacggtggcg
    13381 ttctcgccgg cgtcgtacca ggcttccacg aggaagtggt cgctggcgtt cgaggaagcc
    13441 gcataagtgg gcacgttgac cgtcatgctt ccggcgggct gggaggtcga ggccgtccat
    13501 tccgcgtggc gcgcgcggcc gtattcgttg ccggccgcgg ttaccaggat cttccccggg
    13561 ccggtcagtg ccgtgagggc gatgtccatc gggtccgaac cgtcatgagg gcccgtcatc
    13621 ttgttgaccg ccaggaggac caccgccggc ttgttgagag cggctgcctt ctggaacacg
    13681 taattcacgc cgtcgatgat ccgggcgtcc gtgtaggtgc cgttgggatt ggccatgagc
    13741 ttcacgaaga cgatgtccgc ctccggcgcc atgccggtgt aggtgaactg cggaacgcca
    13801 ccgccggtgg cccggccgtt acccgccgcc acgccggtga tgtgcgtgcc gtggccgact
    13861 tcgtccaggc ccgggtagct cccggcgttg atctgcgcct gggtgtactc gctgccatag
    13921 gtgaaaccgg ccggcttctg ccccgtcgtg atcgtctggt cccagccgaa ttttacgcgc
    13981 gagccggcgc tggttctcag gtcctggtgg gtgacgtcga ggccggtgtc cacgatgccg
    14041 atcaccacgt tttcgccggt ctggcccgtc gcggcatagt tcgggggcgg gccgccccag
    14101 agggccttgg cgttggtcgc aagggtgctg atgtccgtgc agagttcgac aggctgttcc
    14161 atttcgaggc cgcggatccc gtcgagcgcc aggatgtccg gcacgctcga cagcggcgcg
    14221 agaagcgtgg tgacgccgcc ggcgctggta ttgacctcgc cattcaggga gctcagttgg
    14281 gacagatcga cattcccttc gaccacaagg aggacacggg agtccggagt ggcccagtgg
    14341 tagtccgggc cgccggaaac gatctccatc tcgggccgga cggagttcgt ctggagttca
    14401 gtggcgacat tttcgacggc cgccgcggcg ctgagtgccg accggaggtg cacggaaacg
    14461 cggggatcgt tgatggatgc gcgaagggat ccggcaagga gggacgccgg aaagaggagg
    14521 agggcgagga tggtgaacac gacaaggttc aggggcatcc agtccttgac cagtccatgc
    14581 agggaccggt gcgtctgcgg gtatcgcaaa tttgacctcg atgacagggg gtggaacgtg
    14641 tcgcgtcagc cggcaggcgg ccttacgccg aacgccgacg gccccgtcgt caaattcgcc
    14701 ggattcccca ccggaccgtc ggacgtcagg caacgcactt ttggtgccat tccccattta
    14761 ccccctgcat acgaagccat gtggcaagct tgtatgcaga taacccatct acaccctact
    14821 ggggggtttc cccaatggtc aacccatttt cgcggttcgc cacacattta ttgacaattg
    14881 caattcccgg taaaagtgac ccaggttatt gatcgataac cggttgcgat atggttgtca
    14941 atcaggttga acgtgatgcc accctgacct gacacggcaa tcggtcaggc cggaacaggg
    15001 tgtggccgat aatcagtgtc cgttcctccc gatttcagcc cgctacaatg acttgcctcg
    15061 tcaggcctgt catgttctcc ggagaccaga tgttcctgac ccgcggatcg cccgttgtgc
    15121 tcctcctggc cctgctgggg gccgcgtgcc tcatgggtgc cgccgcggga gcggccgaaa
    15181 cccctccgga ttcgaccctg gaaacagcta ttcgagccct tgtcgaccag ggtctcgaga
    15241 acgtctcgat ggcgcgattg accgacgggg ggctcgacgt ccgctacgaa aaccggatcg
    15301 agcgctggga cctgccggcg atcgggcgcg tggcagaggt gtgccggagg gcggtggacg
    15361 gcgagcgctc gctggccgtc acgccgctgg ttcgcggcgt ccccgttcgc accctcacgg
    15421 ccacggccga tgactggggc cggttcctcg ccggactcac gtccgccgcc gccttccggg
    15481 aacgccttgc cattacctcg cctgcccgga ggggcccggc gacgccgagg accaattctt
    15541 cgcacgggcg gatcgacctg gcccttcgtc cgctgctcga tatccagctc gggatcgccg
    15601 acgatccgtt ccagaccgcg gtggcggtgg cgcccgagct gaccatgagc ccgttacgcg
    15661 gcttcctgct cacgctgcag gcccgcctcg agctgcagga cgacctggat gacttcaaca
    15721 acccacttga accgggccgc aacacgctct cctggaatgg ccggctgccc gccgggctcg
    15781 aggtggcaac cagcgccggg actttcccgg agaaccgcta cggcgtggta gccgagatgg
    15841 ggcgatacct cgagccgggt ggacggctgg aggcccggat cggcggcgac tactcgggct
    15901 tctacaagtt tctggacggc gggacgattt cctactccga cgcgggagcc tggtcgggga
    15961 tcggcacgct catcggccgg attcccgacc gcgagatcga gctggccgtg gccggcgggc
    16021 acttcctgac cggggataca ggggtccgcg tcgacctgct gcgccgtttc ggggaggtgg
    16081 agctcgggtt cttcggcgtc tcgaccgacg aaggggaagt ggggggcttc cggttcagcg
    16141 tgccgctgcc ggtgcgccac tggtcccggc catcctcggt tcgcctcgtg acggtcccgt
    16201 cattcccctt cgagtaccgg gaagacgtca caccgatcgg tcgccgcgtc cgggcctacg
    16261 acacgctcga ccgcttccgg cgcggtgtga cacccgtcgg gatcgccacg aacatcgagg
    16321 cgatccgggg gcgttcggtg acggcccgct tccaggcccg ggggctcccg ggggagccgg
    16381 tggccgaatc ggggccggcc ctcggcggca cgaccggact catcgacatc ccggtgccgt
    16441 atgtcacgcc ggaggggacg ctccacgtcg gatacagcca tatcccggag aagtgggcct
    16501 acgaggggcg ccccaccctg aagaacgact actggtacct gacggtgggg atgctgccgt
    16561 ggatggaaac ttccattcga gccaccgtgc tccccggcga atacctgatt gacgacgttc
    16621 cggtcgacgc cgtcgatcga atggggagtc tccgtatcca gagtccctgg ccgaggggtc
    16681 ggaccaccct cggggccgga attgatgaca ttcggggcac aagacgcttt cattctctgt
    16741 atctcgttgg agcccaatca tttgagcccc atttcaccag tatgggcctg gaagtcgtcg
    16801 caggatatgg attgcgcagc ctgtccgctc cccgttatct tctagatggt gcctttggag
    16861 gtgtccgttt gcgactcact ccctgggcct tcgcgatggc cgaacatgac agcgagaaat
    16921 ggaacggtgg acttcgcgtc gttgcgtttt cccgcctgtc ggcccagttg gcaatgctca
    16981 atttcgagtc cttgtcggct ggcgcatcct ggaccgttcg attctgatgt attgcggctg
    17041 agtcgcagca cacctggggg atcaacgcat gacgctccgc aacaagaaat ggcagtggcc
    17101 ggcaacggcc gcactggcga tcacgctcct gacactctcc ggctcgcagt gcagtcaggt
    17161 ggccgaccgc gccaccggac ctgacactgg ttcgctcggg aacaacaacg acgtcgccgc
    17221 ctgcgtacag gcgtgcaacg aaaccgcgaa gcaggaccgc gatgcggaaa aggacctgtt
    17281 caaggccaac aaggaagcct gcaacgggga tccagcctgt ctcgacgcgg aagaagcacg
    17341 gcacgaggcg cgcatggaag aaatcgcggc ggcatcgcag ctctgcaagg ctccctgtca
    17401 cgaacagggt ggcggcggcg gcggtcagta acgcgcaccg cgacgacgag ttgaactgaa
    17461 cttttcggcc cggccggttt cactggccgg gccgattcgt tcttgtgagg cggctgcctc
    17521 tcctcgaaac ccccgatgac gtggccgtgt gagccggcgg gctcagcgca cctggtggat
    17581 cttgaggaag ttggtcgatc cggcctggcc tacaggtgtg cccgcggtca cgacgatccg
    17641 atcgccggga tggatggccc cttccttccg cagcgcgccg gccacccact cgaccatgcg
    17701 gtcggtcgtg gagacggccg gtgcgggccg cgactccacg ccccagagga gcgccatccg
    17761 gcggcggatg cggtcggagg aactgaacgc gatgagacgc gcgcccggcc ggtactgcgc
    17821 cacgcggcgg gcggtgtttc cggtctcggt gaagacggcc acgaccgggg cgccgagccc
    17881 ctccgcggca ttggccgccg cctcgcagat ggcgtcggcc accgacttgc gccaccacga
    17941 ggcactctcg gcctcccgtc gcgggttgga ggccagctcg ttctccacgt tctcgatgat
    18001 gcggcccatg agctcgaccg tctcgaccgg atattttccc acggcggttt cggccgacag
    18061 catgacggca tcggtgccgt catagatggc gttggccacg tcgctggcct cggcgcgggt
    18121 ggggcgggga tgctcgatca tcgactcgag catctgggtg gcggtgatca cgggcacgcc
    18181 gcggcgacgc gcctcgcgga tgacccgctt ctgcagcacc ggcacctgct ccgggctcgt
    18241 ttccaccgcc aggtcgccgc gggccaccat gacggcgtcg gactcgccga ggatctcgat
    18301 gagattggcg atggcctgcg gcttttcgag cttggccacc accggcaggt ccggcgcctt
    18361 cagacgcttg agcatggagc ggaggagccg gacatcatcg gccgagcgga cgaatgagag
    18421 ggccatccag tcgacaccga gccggacgcc cagcttcagg tcctcccggt ccttgggcgt
    18481 gagggctgcc accgagagct tgcggccggg gatgttgatc cccttgttcg agccgagcat
    18541 ccccccgtcg acgacccggg tgtgcacgtc ggtggtctcg acgtgcgcca cctcgagctg
    18601 gatcaacccg tcggccagga ggagctgatc tccccgcgtt acttcccgtg gaaggtcctc
    18661 gaacgtggtc gacacgatgg ccgcgcttcc cggcaccctg cgggtggtga ggacgaacgg
    18721 cgcgcccggc ttgagcagga cttcaccggc ggccatgagg ccggtgcgga tcttcggccc
    18781 ctggaggtct tgcaggatgg ccaccgtggc accgagccgg cgcgcttccc gatggatcga
    18841 ctcgaccgtc cgctggtgct cgtcgtgcgt gccatgggaa aaattcaaac ggcatacgtt
    18901 catccccgcc tcgatcaggc ggcggatcat tttcggctcc cgtgtggccg gccccagtgt
    18961 tgcgacgatc ttcgcgcgtc tcatgcagcc gggtatgcta acggaccgat gacgccaccg
    19021 ccaatgctcg gccgctggat ggcggtccaa cttcccttgc tgggcctcct gatgaccatg
    19081 gccatggccc ggcttgcccg gttcccgacg ctggcggggc agggagccaa cctgcttctt
    19141 ctcctggtga tcctggtggc ctgtttctgc gcggccttgt tcgtggcacg acggctctgc
    19201 cggtcggacg cggccgacgg gcgcgggatc gccggagtgg tgctctcctt tccggtgctc
    19261 ctcaccgtcg gaattctcat gcggctgccg ctcctcgcgg cgccgcctca actctccgat
    19321 gacatctacc gctacgcctg ggacggccgg gtggcggtgg ccggtacgaa tccctatcgt
    19381 tacgcgccgc ccgacccggc gctgtcgggc ctgcgtgacg ccgactggcg gcgcatcaac
    19441 aacccgacgc tccccacgat ctatccgccg gtggcacagc gcctgttcga agtcgcgacg
    19501 cgcatcgcgc ccggcgtgac cggcctcaag tgtctcttct tcctgttcga cctgtcggtg
    19561 gcgttgctcc tgtcgattac agtggcccgc tcgttcgcct cgcggtggcg tctcctcgtc
    19621 tggtggtggc atccgcttgc ggtaatcgag tggtccgggt cggggcacgt cgatgtggcg
    19681 ggcattgcgc tcctggcggc cgcgctcgcg ttggccgcgg cgccggccgg gtacgcgcgc
    19741 tggctggtga cgggactcct ggtgggtctg gccacgcagg tcaagttcct ggcactgctc
    19801 gcacttccgt tcgtccccgg cgccgcgagc cggaagagcg gggggtgggt ggcggcggga
    19861 ttcgtccttg ccctcctggt cggttacctt cccttcatgg cccccggcgt gaacgtcttc
    19921 ggcagtctgg aaacgtacgc ggcccgctgg cgcgccagcg atttcttctt cggattgatg
    19981 atccggtccg gcaccgcgca ggaccagccc gcacgcttga ccgacgccaa gctctttgtt
    20041 gccctgctgg ccggcgcaat tgcgacgggc gtggctctcc tccggacccg acccgcggcc
    20101 gcggtggcca cgaccatcgg ggcggccgtg gttctctcgc cgacggtgca tccctggtac
    20161 ctggcctggg tgcttccgtt cgtggccgcc gaattctcga tcccgtggat ctacgccacg
    20221 ctggccgctc ttttggccta tcaggcggtc ccggcctggc tggccggaca tgtctggtcg
    20281 gagtcggcct ggcagaaggg agcgctcgtg cttccgttcc tcgcgctggc ggtgtgggag
    20341 ctggcccgct ggcgggccgg tcggcaggag atggcgcaat gagcgcggcg ccttcgtcca
    20401 ggaccggcgt gacgctggtg atccgcacac tgaacgcggc tccgtggctt ccggaactcc
    20461 tgccgcgcct ggccgaacag cgacgccggc ccgacgagtt gctggtggtc gattcgggat
    20521 cgacggatgg caccgtggag cgactgctgg ccgcgggccc cgcgatcacg tcgcccgagc
    20581 cggcaccagg cgacgcggag cgggcacgcg tcgtcacgat tccaggccgc gagttcagtc
    20641 acgcgcgaag tacgaacctc ggattccggg aggcgcgggg cgcgatcgtc gtcatgatca
    20701 gccaggatgc gctccccgcg agcccggagt ggctgtcgca actgctggcg cccctcgaat
    20761 cggtgcgagc gggcgatggg acggtcgtcg ccgccttcgg tcggcacctt ccccggcccg
    20821 gagcgttcgc gctcgaacgg tggcagatcg aggccgacta tcccgcgggt ggagcggccg
    20881 gagtcctgtt cagcaacgtg aactccgcca cgcgccgttc ggcgtgggtg aacgagccgt
    20941 tcgacgagtc cctcaccatc gccgaggacc gcgtgtgggc ggccggccag gcggcgcgtg
    21001 gccgacgcat tgtctacgtc cccgaagcga cc
//
LOCUS       DASVOD010000230         1549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_328316, whole genome shotgun sequence.
ACCESSION   DASVOD010000230 DASVOD010000000
VERSION     DASVOD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328316"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VF720_04895"
     CDS             <1..141
                     /locus_tag="VF720_04895"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX7878721.1"
                     /translation="ESPGGWRLIGRTPVSLFDPMADPPALLGIGDVVRFKSITAREYE
                     GR"
     gene            138..1091
                     /locus_tag="VF720_04900"
     CDS             138..1091
                     /locus_tag="VF720_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX7878722.1"
                     /translation="MSFLVVEKPGLLTTVQDAGRPRLAHLGISPAGAADDLAAKAANR
                     LVGNRDSAAVLEMTWTGAQLAFHGPAVVAAVGAPMPLTIDGTPMPAGRAVQLKPGVKL
                     VAGAARQGARLYLAIRGGLEVPMTLGSAATHLLSGLGNPGRALRAGDMIAFGAAGHDL
                     GQAIDAPASRTRAFLAEAWSARALAVTPGPQHDWFTRAAHDAIGIAEWTVHDHSDRLG
                     IRLTGSTLDRTDDRELLTEGVLLGSIQVMPDGSPVILFVDQQTTGGYPKIATVASVDR
                     HRLGQLRPRDTVRFEWVTFEEARQRRAVRDASLDAALPPSP"
     gene            1106..>1549
                     /locus_tag="VF720_04905"
     CDS             1106..>1549
                     /locus_tag="VF720_04905"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX7878723.1"
                     /translation="MNHTRSIDLNADVGEIPELQSNGTEARLIAAVSSVNIACGGHAG
                     DISSVETAVRTAIAHGVAIGGHPSYPDRANFGRVVMEITHHDLMRDIYRQVVSLMSVA
                     REHGAVVRHVKPHGALYNAAVDDANLAAVIAKPLAEWRDSLTLVGL"
BASE COUNT          240 a          545 c          525 g          239 t
ORIGIN      
        1 gaatcgcccg gcggctggcg gctcatcggt cggacaccgg tttcgttgtt cgaccccatg
       61 gcggatccgc cggcgcttct cggcataggc gatgttgtac gcttcaagtc catcaccgct
      121 cgcgaatacg aaggacgatg agcttcctcg tcgtcgagaa gccgggcctt cttaccacgg
      181 tgcaggatgc ggggcgcccg cgcctcgcgc acctcgggat ctccccggcg ggggccgccg
      241 acgacctggc ggcgaaagcg gccaaccggc tcgtcggcaa tcgcgactcg gcggccgtgc
      301 tggagatgac ctggacgggc gcccagctgg cgtttcacgg tccggcggtc gtggcggcgg
      361 tgggcgcgcc gatgccgctc acgatcgacg gcacgccgat gccggccggt cgcgccgttc
      421 agttgaagcc gggagtgaaa ctcgtcgcgg gcgccgcacg gcagggcgcg cggctttacc
      481 tggccattcg cgggggactc gaagtaccca tgacccttgg gagtgcggcc acgcaccttc
      541 tctccggact cggaaacccg gggcgtgccc tccgcgcggg cgacatgatc gccttcgggg
      601 ccgccgggca cgacctgggc caggcaatcg atgcgccggc gtcccgcacg cgggccttcc
      661 tggccgaggc gtggtcggct cgcgcactgg ccgtcacccc ggggccgcag cacgactggt
      721 tcacgagagc cgctcacgac gccatcggca ttgccgaatg gaccgtgcac gaccattccg
      781 accgtctcgg catccgcctc accggatcga cactcgatcg caccgacgac cgggagctcc
      841 tcaccgaagg ggtgctgctc ggctcgatcc aggtcatgcc ggatggatcg cccgtcattc
      901 tcttcgtcga ccagcagacg accggcggct atccgaagat tgccacggtg gcatcggtgg
      961 atcgccatcg gctgggccag ctgcggccgc gtgataccgt tcggttcgaa tgggtgacgt
     1021 tcgaggaagc gagacagcgg cgcgcggtgc gcgacgcgtc cctcgacgcc gcgctgccgc
     1081 cgagtccctg agcttgcggg aaacaatgaa ccacacgcgc tcgatcgatc tcaacgccga
     1141 cgtcggggaa atccccgaac tccagtccaa cggcaccgaa gcgcggctca tcgcggccgt
     1201 cagctcggtc aacatcgcgt gcggcggaca cgccggcgac atctcgtcgg tggaaacggc
     1261 cgtccggacc gccatcgccc acggcgtggc catcggcggc catccctcct atccggaccg
     1321 cgcgaatttc ggccgcgtgg tgatggagat cacgcaccat gacctcatgc gcgacatcta
     1381 tcggcaggtg gtgtcgttga tgtcggtggc ccgagagcat ggcgcggtcg tccgacacgt
     1441 gaaaccgcac ggcgccctct acaatgcggc cgtcgacgac gcgaacctgg cggccgtgat
     1501 cgcaaagccg ctcgccgagt ggcgcgacag cctcacgctg gtcggcctc
//
LOCUS       DASVOD010000231         3929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_277041, whole genome shotgun sequence.
ACCESSION   DASVOD010000231 DASVOD010000000
VERSION     DASVOD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277041"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="VF720_04910"
     CDS             complement(<1..1222)
                     /locus_tag="VF720_04910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878724.1"
                     /translation="MPLTPGTRLGPYDIIAPLGSGGMGEVYRARDPRLGRDIALKTLP
                     ADVAGDAQRLARFQREARTVAGLSHPNVVVLHSIEEADGMHFLTMELVDGEGLDTVVV
                     PGGLPFPRVLELAIALSDALTAAHERGIVHRDLKPANVMLTRDGRLKVLDFGLAKPSD
                     SSSTPDLSRAMTLDRPVSTVGQIVGTVPYMAPEQIRGEPTDSRTDLFSLGILVFELAT
                     GRRPFSGGTAADISSAILRDAPPTLGELRAELSGDFDRIVSRCLEKEPRDRFQTARDV
                     HNELRRLRKSIESSGMTAARPAVTESPSIAILPFTNLSRAEDDEYFSDGLADELLNML
                     ARIRGLRVAARSSSFAFKGRNATVAEVGQALGVATVLEGSVRKSGDRARISVQLVKVA
                     DGFHLWSERYDRTLD"
     gene            complement(1266..1415)
                     /locus_tag="VF720_04915"
     CDS             complement(1266..1415)
                     /locus_tag="VF720_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878725.1"
                     /translation="MGADLDLDLVWKALADPTRRERWVTGYRDLWVRQLTSLKRSLGE
                     SAGNS"
     gene            complement(1457..1900)
                     /locus_tag="VF720_04920"
     CDS             complement(1457..1900)
                     /locus_tag="VF720_04920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7878726.1"
                     /translation="MRVLLALDESECSRKAIESVRRMTWPSGTEFLVLSVAPTRFLLS
                     AEYYVDSMDIDAAEKAALVAHEKVATDGQRVLEGVSGPVRRVVRTGDPRIVILEVAQS
                     EDVDMIVIGSHGRTGLARLFLGSVAAHVVTHAPCNVLVVREPRPT"
     gene            complement(1946..2992)
                     /locus_tag="VF720_04925"
     CDS             complement(1946..2992)
                     /locus_tag="VF720_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018879012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX7878727.1"
                     /translation="MRAAAFVKPGKIELQERPIPEVGPKDALLRVTMTTICGTDVHIL
                     KGEYPVAPGRIIGHEPVGVIEKLGSAVEGYAPGDRVIAGAITPCGQCFACLDGHPSQC
                     GGRKMGGWRLGNTIDGCQADYVLIPDAAANLARVPSGLTDEEVLMCPDIMSTGFSGAE
                     SGNVRIGDVVAVFAQGPIGLCATVGARLRGASLVIGVDGIPERLRLASQLGADMTINY
                     RETDPVEAIMKLTDGRGVDVAIEALGTQGTFENALRVLRPGGTLSSLGVYSGKLSVPL
                     EAFAAGLGDHRIVTTLCPGGKERMRRLLNVVNSGNVSLKPLVTHRFPLERLEEAYALF
                     ASQEKGVLKIAITP"
     gene            complement(3058..3558)
                     /locus_tag="VF720_04930"
     CDS             complement(3058..3558)
                     /locus_tag="VF720_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7878728.1"
                     /translation="MRIKDIMSHPVVTASLGASMDVVARRMWEHNCGAIVIVDEDDRL
                     AGLITDRDICMAAYTQGKPLHAIAVTSAMAREVSTCREEDSLHEAEKVMCSRRVRRVP
                     VVNAHGVPVGMVTMDDLARNVTGTKGGRKTAQETAVLHTLAAVCGPPSDRGVESEEHR
                     RKAGGG"
     gene            3741..3908
                     /locus_tag="VF720_04935"
     CDS             3741..3908
                     /locus_tag="VF720_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878729.1"
                     /translation="MTRFPSAGSLKPGTWSPAHHVELTGASGNVPYRPSNVARAESMG
                     TSATDFTPNSP"
BASE COUNT          633 a         1320 c         1296 g          680 t
ORIGIN      
        1 cgtcgagcgt gcgatcgtag cgttcggacc agaggtggaa gccgtcggct accttgacca
       61 gttgcaccga gatgcgggcg cgatcgcccg acttgcggac acttccctcg agcacggtgg
      121 ccacgccgag cgcctggccc acctccgcca cggtggcgtt gcgccccttg aacgcgaagg
      181 acgatgagcg ggcggccacg cgcaggccgc ggatccgggc cagcatgttg aggagctcgt
      241 cggccaggcc gtcggagaag tattcgtcgt cctcggcacg gctcaggttg gtgaacggga
      301 ggatggcaat cgacgggctc tccgtgacgg ccggccgcgc ggccgtcatg ccgctcgact
      361 cgatcgattt ccgaaggcgg cgcagttcgt tgtgcacgtc acgggcggtc tggaaacgat
      421 cgcgtggctc cttctcgagg cagcggctga cgatgcgatc gaaatcgccc gacagctcgg
      481 cgcgcagctc cccgagcgtg gggggcgcat cccgcaggat ggccgagctg atgtcggccg
      541 ccgtgccacc cgaaaacgga cgccgccccg tggcgagctc gaagacaagg atccccagcg
      601 agaagaggtc ggtgcgcgag tcggtgggct cgccgcggat ctgttcgggc gccatgtacg
      661 gaaccgtgcc gacgatctgg ccgaccgtgg aaaccggccg gtcgagcgtc atggcccgtg
      721 acagatcggg cgtggacgag ctgtcgctcg gcttggccag gccgaaatcg agcaccttga
      781 ggcgaccgtc ccgggtgagc atgacgttgg ccggcttgag gtcgcgatgc acgatgccgc
      841 gctcgtgggc ggcggtgagc gcgtcggaga gcgcgatggc gagctccagc acgcgcggga
      901 acggaaggcc gccgggcacg acgaccgtgt cgagtccctc gccgtcgacc agttccatgg
      961 tcaggaagtg cataccatcc gcttcctcga tggagtgcag gaccaccacg ttcgggtggc
     1021 tgagtccggc cacggtgcgc gcttcgcgct ggaagcgggc caggcgctgg gcatccccgg
     1081 ccacgtcggc agggagcgtc ttcagggcga tatcgcggcc caggcgcgga tcgcgggcgc
     1141 gatacacctc ccccatgccg ccggatccga gcggggcgat gatgtcgtag ggaccgaggc
     1201 gcgtgccggg agtcagtggc atggcgggag tgttgcagcg gcgggacgtg gttgtcgagc
     1261 cggcgtcagg aatttccggc cgactctccc agcgatcgct tgaggcttgt caactgccgg
     1321 acccacaggt cccggtagcc ggttacccat cgttcgcggc gcgtgggatc ggcgagcgcc
     1381 ttccagacca ggtcgaggtc gaggtcggcg cccatgcgat cgggctcgcc ggggtaacga
     1441 cggtccgctt tccggatcag gtcgggcgcg gttcccggac caccagtacg ttgcacggcg
     1501 cgtgggtgac gacgtgcgcc gccacgctac cgagaaacag ccgcgccagg ccggtgcggc
     1561 cgtgggagcc gatcacgatc atatcgacgt cttcggattg tgccacctcg aggatgacga
     1621 tgcgggggtc tccagtcctg acgactctcc gtaccggacc ggacaccccc tccagcaccc
     1681 tctgcccatc ggtcgcgacc ttttcgtggg cgacgagggc cgccttctcc gctgcgtcga
     1741 tgtccatcga atcgacgtag tactccgcgc tgagcaggaa gcgggtcggc gcaaccgaca
     1801 ggacgaggaa ctcggtcccg gagggccagg tcatccgtcg aacggactcg atggccttcc
     1861 tggagcactc ggattcgtcc agtgcgagaa gaactctcat ggctgcctcc atgggttgtt
     1921 ggatgcgccg acccggaaag ggggcttacg gagtgatggc gatcttcaac acccctttct
     1981 cctggctggc gaacagggcg tacgcctcct cgagcctctc gagcggaaac cgatgggtga
     2041 cgaggggctt caggctgaca ttgcccgagt tgacaacgtt gagcaaccgc cgcatccgtt
     2101 ccttgccgcc aggacagagg gtggtcacga tccggtgatc cccgaggccg gctgcgaacg
     2161 cttcgagcgg aacggagagc ttgccggagt agacgccgag gctggacagg gtgccccctg
     2221 gacgaagaac ccgaagcgcg ttctcgaagg tgccctgggt tcccagcgct tcgatggcca
     2281 catcgacccc gcggccatcg gtcagcttca tgatggcctc gactggatcc gtctcacggt
     2341 agttgatggt catgtcggca cccagttggc tcgcgagccg gagccgttcg gggatcccgt
     2401 caacgccgat gacgagcgag gccccccgca gcctggcccc gaccgtggcg cagaggccga
     2461 tcgggccctg ggcgaagacg gcgaccacgt cgccgatccg gacattgcca ctctcggccc
     2521 ccgagaatcc cgtactcatg atgtcgggac acatgagcac ttcctcgtcg gtgagcccgg
     2581 aggggacccg ggccaggttg gccgccgcat ccgggatcag cacatagtcg gcctggcagc
     2641 catcgatggt gttcccaagc cgccagcccc ccatcttccg tccgccgcac tgcgacggat
     2701 gcccgtccag gcaggcgaaa cactgcccgc agggagtgat ggcgccggca atcacccggt
     2761 cgcccggcgc gtaaccctcg acggccgatc cgagcttctc gatgaccccg accggctcgt
     2821 gcccgatgat ccgtccgggc gccaccgggt actccccctt caggatgtgc acgtcggtcc
     2881 cacagatcgt ggtcatcgtg acgcgcagga gcgcatcctt cgggccgact tccgggatgg
     2941 gcctctcctg caactcgatt ttcccgggtt tcacgaacgc ggcggcccgc atcatgagcc
     3001 tgccggaact ccggcgcgtc gtcggcgccg gtgcctgaat gacctggcgg tcgctgttca
     3061 tcctccacct gcctttcgtc gatgctcttc cgactccacc cccctgtctg atggtggtcc
     3121 gcacacagct gccaaggtat ggagtaccgc ggtttcctgg gcggttttcc tgcccccctt
     3181 cgtgcctgtg acgttccggg ccaggtcgtc catcgtgacc atgccgacgg ggactccgtg
     3241 ggcgttgacc accggcacgc gacgcactct tcgggaacac atcacctttt cggcttcatg
     3301 gagtgagtct tcctcgcggc aggtggacac ttcccttgcc atcgcacttg taaccgcaat
     3361 agcatgaagc ggcttgccct gcgtgtaggc cgccatgcag atatcgcgat ccgtgatcag
     3421 cccggcaagc cgatcgtcct cgtccacgat cacgatcgca ccgcaattgt gttcccacat
     3481 ccttctggcc accacatcca tgcttgcccc aaggctcgcg gtgacgaccg ggtggctcat
     3541 gatgtccttg atccgcatga ccatctcctc tccggggatc cctgtgacct cctgttcccc
     3601 acgtctgctt gcatcaggaa tgccatggcg ttgcgatcca tttcgtggcg cttggccggg
     3661 cacctcgctg gaatctcacg aggcattgac ttcctgtcat gacaggccag ctcgaccggc
     3721 agccactatc caggagttcc ttgacccgtt tccccagcgc cgggtcgttg aaaccgggta
     3781 cctggtcgcc ggcgcaccac gtcgaactca cgggcgcctc cggcaacgtg ccgtatcgcc
     3841 cctcgaacgt agcccgggcg gagtcgatgg gcacaagcgc cacggatttc acgccgaact
     3901 caccgtagga aggatgccag ccttcacgg
//
LOCUS       DASVOD010000232         3067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_277129, whole genome shotgun sequence.
ACCESSION   DASVOD010000232 DASVOD010000000
VERSION     DASVOD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277129"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VF720_04940"
     CDS             <1..1122
                     /locus_tag="VF720_04940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HEX7878730.1"
                     /translation="GGASGLIVENFGDAPYFPDQVPPETIAAMTRLVTEVRKAVRIPV
                     GVNVLRNDARGGVAVAAATGAGFVRVNVHTGVMVTDQGLIAGRAWESVRLREVLRSRT
                     LLFADLRVKHAAPLADFDLVAEADDLLSRGRADALIVTGAATGSPADLSRVKALKEAF
                     PEVAVMVGSGVDAENAEAVLAIADGAIIGTAIKYDGVVGNAVDSERLSVITRVASRLA
                     PRESADPQRNEERPRYEDRPRYEERPRNEERPRFEERPAFDERPRRSERPAPREHYAG
                     NGETPAAPPREKEQPVESQAPWSAAPARDGGVPVWEPRPLAGRPSPVLTDAVEELDPI
                     VDTTAPVSPAAPAVAVTPSASAAEEPAAFGRIGHRKKRR"
     gene            1253..2155
                     /locus_tag="VF720_04945"
     CDS             1253..2155
                     /locus_tag="VF720_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEX7878731.1"
                     /translation="MNLIDQAVVGLLPLVPKPIVRRFSSRYIAGDDLAAAVRTIRLLN
                     GRRLMATVDVLGEFIHRLDEADATADEYEQVLSAIESGGLDANISVKLTGLGLLLDRE
                     HCYRLVERLVRGAAARKNFVRIDMEDSACTSITLDLYRRLREAGHDNVGVVLQAYLRR
                     SHDDIAALLPLKPNVRVCKGIYVEPEAIAYKDADEIRMNYRRMVERLLDAGCYVGMAT
                     HDPALVETGKAIVKARNLPTSAYEFQMLLGVAEEIRQSLIDAGHRLRVYVPYGAQWYA
                     YSVRRLKENPRIAGYVFKNLVSGR"
     gene            2159..2797
                     /locus_tag="VF720_04950"
     CDS             2159..2797
                     /locus_tag="VF720_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX7878732.1"
                     /translation="MKWTRDSGKEPPKSPAVKRVIEDAAKHGSRLPPGQVLTEKWPVL
                     QYSTIPRLDLSMWTFKLWGLVEESALFTWEDFNALPVSTLTNDIHCVTHWSKFDNTWE
                     GVLARDVLARVAIRPEARFAIVHGHDGYTTNVPIDILTGGDSIFAFRHDGMELTPEHG
                     WPCRLVPPPQHYLWKGAKWVSGLEFIAEDRPGFWERNGYHMNANPWREERFS"
BASE COUNT          511 a         1031 c         1026 g          499 t
ORIGIN      
        1 ggcggcgcct cgggcctgat cgtcgaaaat ttcggcgatg ccccctactt cccggaccag
       61 gtgccgcccg aaacgatcgc ggccatgacc cggctcgtca cggaagtgag gaaagcggtc
      121 cggatcccgg tgggcgtcaa cgtgctgcgc aacgacgcgc gtggtggcgt ggccgtggcc
      181 gcggccaccg gcgccgggtt cgtccgggtc aacgtgcaca ccggcgtcat ggtcaccgac
      241 caggggctga tcgccggccg ggcctgggag tcggtgcgcc tgcgcgaggt cctgcgttca
      301 cgcacgctgt tgttcgccga tctccgcgtc aagcacgccg ccccgctggc cgacttcgac
      361 ctggtggccg aggccgacga cctcctttcg cggggccggg ccgatgccct catcgtgacc
      421 ggcgcggcca ccggctcgcc ggccgatctc tcccgggtga aggcgctcaa ggaggcgttc
      481 cccgaggtgg ccgtcatggt cgggtcgggc gtcgatgcgg aaaacgccga ggcggtgctc
      541 gccatcgccg acggcgcgat catcggcacc gccatcaagt acgacggggt cgtcggcaat
      601 gccgtcgact cggaacgcct gtcggtgatc acccgcgtgg ccagccgcct ggccccgcgc
      661 gaatccgccg atccacagcg gaacgaagag cggccccgtt acgaggatcg ccccaggtac
      721 gaagaacggc cccggaacga ggagcgcccg cggttcgagg aacgccccgc attcgatgag
      781 cgtccccgtc gttcggagcg ccctgcgccg cgcgagcact acgccggcaa cggcgagact
      841 ccggcagcgc cgccacgcga gaaggaacag ccggtcgaat cccaggcccc atggtcggcg
      901 gcacccgcgc gcgacggcgg tgtcccggtc tgggaacccc ggccactggc cggccggccg
      961 tcaccggtcc tgaccgacgc agtcgaggaa ctcgatccga tcgtcgacac cacggctccg
     1021 gtcagtcctg ccgccccggc tgttgcggtc actccttcgg catctgccgc cgaggagccc
     1081 gcggcgttcg gacggatcgg acatcgcaag aagcgccgct agtcgttccg gccgacacgt
     1141 tcaagtcatc atcaggcccc ggtcccgaga gggatcgggg cctgttagct gctcggagcc
     1201 gaggccgggc cggcgctgat tttgcatcgc caagatgata gagtcgcatc ccgtgaatct
     1261 catcgaccag gccgtcgtcg gcctcctgcc cctggttccg aaaccgatcg tccgtcgatt
     1321 ttcctcgcgg tacatcgccg gtgatgacct ggcggcggcc gtccgcacga tccggttgct
     1381 gaatggccgc cggctcatgg ccacggtgga tgtcctcggg gagttcatcc accgactcga
     1441 cgaggcggat gccacggctg acgagtacga acaggtcctc tccgccatcg agtcgggtgg
     1501 cctcgacgcc aatatctcgg tcaagctcac gggcctcggc cttctcctcg atcgggagca
     1561 ttgctaccgc ctggtggaac gcctcgtccg gggggcggcg gcccggaaga acttcgtccg
     1621 gattgacatg gaagattcag cctgcacgtc gatcacgctc gacctgtacc gccgattgcg
     1681 ggaagccggc cacgacaacg tgggcgtcgt cctccaggct tacctgcggc gcagtcacga
     1741 cgacatcgcg gccctgttgc cgctcaagcc gaacgtgcgc gtctgcaagg ggatctacgt
     1801 ggagcccgag gccatcgcct acaaggacgc cgatgaaatc cgcatgaact accgccggat
     1861 ggtggagagg ctgctcgatg ccggctgcta cgttgggatg gccacgcacg atccggccct
     1921 ggtcgagacg ggcaaggcaa tcgtgaaagc ccggaatctc ccgacgtcgg catacgagtt
     1981 ccagatgctt ctcggcgtgg ccgaggaaat ccggcaatcg ctgatcgacg ccggacaccg
     2041 gctgcgggtg tacgtgccgt acggggccca gtggtacgcc tactcggtga ggcggctgaa
     2101 ggaaaacccc cgcattgccg gctacgtgtt caagaacctc gtgtcgggcc ggtagcggat
     2161 gaaatggacg cgcgactccg gcaaggagcc ccccaagagc ccggcggtga aacgggtgat
     2221 cgaggatgcg gcaaagcatg gcagccgcct cccgccgggc caggtgctca ccgagaagtg
     2281 gccggtgctg cagtacagca cgattccccg cctcgacctg tcgatgtgga cgttcaagtt
     2341 gtgggggctc gtcgaagagt cggccctctt cacctgggaa gatttcaacg cccttcccgt
     2401 ctccacgctc accaacgaca tccattgcgt gacgcactgg agcaagttcg acaacacctg
     2461 ggagggtgtc ctcgcccggg atgtattggc ccgggtggcg atccggcctg aggcccgctt
     2521 cgcgatcgtg cacggtcacg acgggtacac caccaacgtg ccgatcgaca tcctgaccgg
     2581 cggcgactcc atcttcgcgt ttcgccacga cggcatggaa ctcactccgg agcacggctg
     2641 gccctgccga ctggtgcccc ccccgcagca ctacctctgg aagggagcca agtgggtcag
     2701 cggcctggaa ttcattgccg aggaccgtcc gggcttctgg gagcggaacg ggtaccacat
     2761 gaatgccaac ccgtggcgcg aggagcgctt ttcctgacgc ggtcggcctc cggaaggatc
     2821 cggagatgcg gtaactgagg ctggaaaaaa atcggccggg tcctcgcgga cccggcctcg
     2881 ctttttctgt ttccgttcga ctgaactggc gcgaaccgac tcagtcgatc aggtgcttga
     2941 tgcggctcca ggtggacgtc tgggcgggaa gggcaccgca gtggccgggg cccaggatcc
     3001 cgttgttgta ctggtcgaac atctgcgtgt acatgctcgt ggagctggga gtcaggtagc
     3061 ccgatcc
//
LOCUS       DASVOD010000233         2237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_68239, whole genome shotgun sequence.
ACCESSION   DASVOD010000233 DASVOD010000000
VERSION     DASVOD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68239"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1678
                     /locus_tag="VF720_04955"
     CDS             <1..1678
                     /locus_tag="VF720_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878733.1"
                     /translation="AYELLAGRMPFDIPTDSLHRAIVVILTAEPRPLSEVRPDCRGDV
                     EYIVQRLLEKSPSHRYSSAREVADDLGRVTAGHRLHRLRRPVRRRGSWGMMMWAGVAA
                     VVLGVGAIGWIYARPLLRPERVLFNRSVVASVLHHIADGGLLIHADDATEAVIRQGIA
                     RLDSADVQLQSLPGTMPLAAIRRLLFWRRGEAHYFLGAMKNSKAEFGLARVAWERAWG
                     QWNQPWSLAVVDSSMEMYTELLKTDPSSPIAGMVLVEMALAGYEQPKAHLLQAHDYAN
                     LTWKEMEPEGKALPWQGTTEREMERLHGYCMTLNNLSFIKCRIGWTLGDTNWIAAGLP
                     GLLEADARWPYRRASEPRGAHLHNLGRAYRYRGVLTGSVADLDSAEAAFVRALDYRTA
                     DRLPRGFAATQFERARTVLDRPAPDHRMLEKALRQVRMARTVIEPFADILEAAEGRLV
                     EAELVIRLADRQQLAEADSLIREAGRTITVERFPLQHVDLLRVSALLHEAKSRMSGES
                     AEARMAAQLAGRAVELTSSVEEPFRYRRLVALRSRLESTLGTSGHLRAGH"
     gene            1784..>2237
                     /locus_tag="VF720_04960"
     CDS             1784..>2237
                     /locus_tag="VF720_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX7878734.1"
                     /translation="MKTTTGLEPNPELVLLTGATGYVGGRLLGTLVRGGRKVRCLVRS
                     PHCLQARVIDGVELVQGNVLDLPSTERAMAGVTTAFYLVHSMGSSGSFEDEDRRAARN
                     FGEAAARAGVRRIIYLGGLVPGGVELSSHLRSRLEVGEILRASGVPTIE"
BASE COUNT          339 a          728 c          793 g          377 t
ORIGIN      
        1 cgcgtacgag ctcctggcgg ggcgcatgcc gttcgacatc ccgaccgatt cactgcatcg
       61 cgccatcgtg gtcatcctga cggcggagcc acggccgctg tcggaagtcc ggcctgattg
      121 ccggggggac gtcgagtaca tcgtccagcg actcctcgag aagtcgccga gccatcggta
      181 ttcctcggcg cgcgaggtgg cggacgacct gggtcgggtg acggccggcc accggcttca
      241 tcgcctgcgc cgtcctgtcc ggcgccgcgg gtcctgggga atgatgatgt gggccggcgt
      301 tgccgccgtc gtcttgggcg tcggcgccat cggctggata tatgcccgcc ccctgctgcg
      361 cccggagcgc gtgctgttca accggtccgt cgtggcgagc gtcctccacc acatcgccga
      421 cggcggactg ctcatccacg ccgacgacgc cacggaagcg gtcatccggc agggtatcgc
      481 ccgcctggat tcggcggacg tgcagctcca gtccctcccc gggacgatgc cgctggcggc
      541 aatccggcgg ctgttgttct ggcggcgggg cgaggcccac tacttcctcg gggcgatgaa
      601 gaacagcaag gccgagttcg ggctggcgcg cgtggcgtgg gagcgggcgt gggggcagtg
      661 gaaccagccg tggtcgctcg cggtcgtcga ttcgtcgatg gagatgtata cggaactcct
      721 aaagacggat cccagcagcc cgatcgccgg catggtgctg gtcgagatgg cgctggccgg
      781 ctacgagcag ccgaaggccc acctgcttca ggcgcacgat tacgcgaacc tgacgtggaa
      841 ggaaatggag ccggagggaa aggcgcttcc ctggcaggga acgaccgagc gggaaatgga
      901 gcggctgcac ggctactgca tgacgctcaa caacctctcc ttcatcaagt gccggatcgg
      961 gtggacgttg ggcgatacga actggatcgc ggcgggactg cccgggttgc tcgaggcgga
     1021 tgcccgttgg ccctaccggc gcgcgagcga gccgcgcggg gcgcacctgc acaacctggg
     1081 acgggcgtat cgatatcgcg gcgtccttac ggggagtgtt gcggatctcg attccgcgga
     1141 ggccgcgttc gttcgcgcgc tcgactaccg cacggccgac cggctcccga ggggattcgc
     1201 ggccacgcag ttcgagaggg ctcgcacggt gctggaccga ccggctccgg atcaccggat
     1261 gcttgaaaag gcgctgcgcc aggtccgcat ggcccgcacc gtgatcgagc cgttcgccga
     1321 cattcttgaa gctgccgagg ggcgactcgt cgaggccgaa ctcgtcatcc ggctggccga
     1381 ccggcaacaa ctggccgaag ccgactcgtt gatccgcgag gccgggcgca ccatcacggt
     1441 cgagcgattc ccgctccagc atgtggatct gctccgggtg tccgcgctgc ttcacgaagc
     1501 gaagtccagg atgtccggag agagcgccga ggcgcggatg gcggcgcaac tggcgggccg
     1561 cgccgttgaa ctcacctcat cggtcgagga gccgttccgc taccggcggc tcgtggcgct
     1621 ccggtcgcgg ctcgagtcca ccctcgggac atccgggcac cttcgcgccg gccattaggc
     1681 atctcgatcc cgcttgaatc ccccacgtcc aacttcttga agggtcattt gcccgtccct
     1741 gcatccgttc ctgcgatatc gacgaacagg atgccgagag acgatgaaga cgacgactgg
     1801 ccttgagccg aatcccgagc ttgtcctcct taccggcgcc acgggctacg tggggggacg
     1861 cctgctgggc accctcgtcc gagggggccg aaaagtccgt tgcctcgtcc gctcccccca
     1921 ctgccttcag gcgcgggtaa tcgatggcgt ggagctcgtg cagggcaatg tgctggacct
     1981 cccttccacg gagcgcgcca tggcgggtgt gacgaccgcc ttctatctcg tccactcaat
     2041 gggttcctcc ggatcgttcg aggacgagga tcggcgggcg gcgcggaact tcggggaggc
     2101 ggcggcgcgg gccggggtgc ggcggatcat ttacctcggg gggctcgtgc ccgggggtgt
     2161 ggagttgtcg tcccatctgc gcagccggct ggaggtcggc gaaatcttac gtgcgagcgg
     2221 ggtgccgacc atcgagt
//
LOCUS       DASVOD010000234         3642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_17192, whole genome shotgun sequence.
ACCESSION   DASVOD010000234 DASVOD010000000
VERSION     DASVOD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17192"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF720_04965"
     CDS             complement(<1..552)
                     /locus_tag="VF720_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7878735.1"
                     /translation="MSLLDGVDYKESIIETIGNTPLVRLNKVTRGLKSTLLAKVESFN
                     PGGSVKDRIGIKILTEAEKKGLIKPGGTIVEATSGNTGMGLAIAAAVKGYRAVFVMPD
                     KMSLEKINLLKAMGAEVVVTPTAVPPESPDSYYSVARRLGKEIPNAFLANQYFNDDNP
                     LAHYETTGPEIWKQTAGKITHFFV"
     gene            821..3625
                     /locus_tag="VF720_04970"
     CDS             821..3625
                     /locus_tag="VF720_04970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878736.1"
                     /translation="MPLAIRRAPLSTLLPSLVLVLVCGPAGAQSFTNFESHQLHPLAL
                     SADGSRLFACNTPDNRLSVYQLTPAGPVLEAEIPVGLEPVSVAPRTANEVWVVNHLSD
                     DITIVDLVTMNVRASIPVGDEPTDVVFAGPAGRAFVVVSQEDAVRILDPANPAQPATV
                     VPLFGSDPRAIAASADGSRVYVSVFESGNNTSILSTAKVGQGGGPPAPNPPRNPALPP
                     PPPVGLVIGWNGLHWVDETGTKNWETLVPIPYSLPDNDLIELDAAQVTPVPRYFTGVG
                     TLNYNLSVNPVDGRVYVPNFAAFNLTRFEPKLRGRFGQYRVSIIDPGPATVTPVNLNP
                     HINTAITPGPPSEIALSLSQVNGGDWSADGQSLYLAALGSGTLAVVNPSGVVTARVAV
                     GQGPTAVQVDDARGLVYVLNRFTNTVATVDRNTLTVTGETSLGYQPEPAAIVAGRKFL
                     YDARLSSGHGDLACASCHAFGNLDALAWDLGDPTGTLAPAPPGFPAFHPMKGPMTTQS
                     LRGLAGTAPFHWRGDRADFTRFNPAFVSLMGKGDTLATVEMQAYADFILTVSYPPNPN
                     QNLDRSLPNPPAPAGSAERGRLQFTGPPHDGPLTCQACHNSAPPNQPFLVSPGTNRLL
                     IPAQALQESQAFKVPHLRNLYEKRGFRDAAGPQKNGFGFIHDGSSDTIVNFLLSPVFQ
                     FANNQQRVDLEAFMHAFDTGLAPAIGAQQTVDGTNGATPAVTARLNLLIAQADAGAID
                     LVVKGRASGQARGWFYVGGGQYESDGVGEPLVTTAELMAGAAAGAERTWTGVPPGAGE
                     RIGIDRDLDGYGDRHELSAGSNPADPNSIPSTSTVGGPVDGPGGRVPTLAASPNPAGP
                     AGTEISFTLADAAEVSLEIFDLSGRRVATLLDGRREQGTVTVHWDGRDDSGRPMAGGV
                     YFSRLAAAGASLTRKIVLAP"
BASE COUNT          574 a         1330 c         1162 g          576 t
ORIGIN      
        1 cacgaagaag tgcgtgatct tgccggcggt ctgcttccag atttccgggc cggtcgtttc
       61 gtagtgggcc agcgggttgt cgtcgttgaa gtactgattg gccaggaagg cgttggggat
      121 ttccttgccg agccggcggg ccaccgagta gtaggagtcg gggctctcgg ggggcacggc
      181 cgtcggggtg acgaccacct cggcccccat cgccttcagg agattgatct tctccagcga
      241 catcttgtcg ggcatcacga agacggcccg gtaacccttg accgccgcag cgatggccag
      301 ccccatcccc gtgttgccgc tggtggcctc cacgatcgtg ccgcccggct tgatgagccc
      361 cttcttttcg gcctcggtca ggatcttgat gccgatccgg tccttgacgc tccccccggg
      421 attgaacgac tccaccttgg ccagcagcgt ggacttgagg ccgcgggtaa ccttgttcag
      481 ccggaccagc ggagtgttcc cgatggtctc gatgatcgat tccttgtaat caacgccgtc
      541 gagaagtgac atgaagccga ctctccaggg aaggcggaac tgtgggccgg tgccggccgt
      601 ccgcgggtgg ttccaagggg ggattcgagc cgaagaagca tagccgcgcc gtttcggcag
      661 cgtcaagccg ccaattgact tagcaaccct ggatcaaagg atggaaagtt tgtaacgccc
      721 tggagggagg ccggtctcaa gcccacatcg gccgcgcggc aacgaatcat ggccccgccc
      781 gacggtcgat gccaccaacc attcctgagg gggagccgcc atgcctctcg ccatccgccg
      841 tgccccgctt tccaccctgt tgccgtccct tgtcctcgtc ctggtgtgcg gcccggccgg
      901 cgcccagtcc ttcaccaact tcgaaagcca ccagctgcac ccgctggccc tgtcggccga
      961 cgggtcgcgc ctcttcgcct gcaacacgcc cgacaatcgc ctcagcgtgt accagctcac
     1021 gccggccggg cccgtcctcg aagccgagat cccggtgggc ctcgagccgg tgtcggtggc
     1081 gccacgcacg gcaaacgagg tctgggtcgt caatcacctc tccgacgaca tcaccatcgt
     1141 cgacctcgtc acgatgaacg tgcgcgcctc cattcccgtg ggcgacgagc cgaccgacgt
     1201 ggtctttgcc ggtcccgccg gccgggcctt cgtcgtggtg agccaggagg acgcggtccg
     1261 tatcctcgat ccggccaacc cggcacaacc ggccaccgtc gttcccctgt tcggcagcga
     1321 cccgcgcgcc atcgcggcct cggccgacgg ctcccgcgtg tacgtgtcgg tgttcgagtc
     1381 gggaaacaac acgagcatcc tctcgaccgc caaggtcggc caggggggcg gcccgccggc
     1441 gcccaacccg ccccgcaacc cggcgcttcc cccgccgccc cccgtcgggc tcgtgatcgg
     1501 ctggaacggc ctgcactggg tggacgaaac cgggacgaag aactgggaaa cgctcgttcc
     1561 gatcccctac tccctgccgg acaacgacct gatcgagctg gacgccgcgc aggtaactcc
     1621 cgtgccgcgg tacttcaccg gcgtcggcac gctcaactac aacctgtcgg tcaatccggt
     1681 cgacggccgc gtctacgtgc cgaactttgc ggcgttcaac ctcactcgat tcgaaccgaa
     1741 gttgcgcggc aggttcggcc agtaccgcgt gtcgatcatc gacccgggtc ccgccacggt
     1801 gacacccgtc aacctcaacc cgcacatcaa caccgcgatc acgcccgggc cgccatccga
     1861 aatcgccctg tcgctctcgc aggtcaacgg cggcgactgg agcgccgacg ggcagagcct
     1921 ctacctggcc gccctcggct cgggaacgct ggcggtggtg aatccgtccg gcgtcgtgac
     1981 cgcgcgggtg gccgtcgggc agggccccac cgccgtgcag gtggatgatg cgcgcggcct
     2041 ggtctacgtc ctgaaccggt tcaccaacac agtggccacc gtcgatcgga acacgctgac
     2101 agtgaccggc gaaacgagcc tgggctatca gccggagccg gccgccatcg tggccggtcg
     2161 caaattcctg tacgacgcgc ggctctcctc cggacacggc gatttggcgt gcgcctcctg
     2221 ccacgccttc ggaaacctgg acgcgctggc ctgggacctg ggcgacccga ccggcaccct
     2281 ggcgccggcg ccgcccggct ttccggcctt tcatccgatg aaggggccga tgacgacgca
     2341 gtcgcttcgt ggcctcgccg gcaccgcccc cttccactgg cgcggcgacc gggccgattt
     2401 cacgcgcttc aacccggcct tcgtcagcct gatgggaaag ggcgatacgc tcgccacggt
     2461 cgagatgcag gcctacgccg acttcatcct gacggtgtcg tatccgccga acccgaacca
     2521 gaacctcgac cgatcccttc ccaatccgcc ggcgcctgcg ggaagcgcgg agcgcggccg
     2581 actgcagttc acgggaccgc cccacgacgg gcctctcacc tgccaggcat gccacaacag
     2641 cgcgccgccc aaccagccct tcctcgtgtc accgggaacc aaccgcctcc tgattcccgc
     2701 ccaggcgctg caggagagcc aggccttcaa ggtgccgcac cttcgcaacc tctacgagaa
     2761 acggggattc cgcgacgcgg ccgggccgca gaagaatggc ttcggcttca tccatgacgg
     2821 atcgtccgac acgatcgtca acttcctgct gtcgccggtc ttccagttcg ccaacaacca
     2881 gcagcgcgtg gacctcgagg cattcatgca cgcgttcgac accggcctcg ccccggcgat
     2941 cggcgcgcag cagacggtgg acggcaccaa cggcgccacg ccggcggtca ccgcccggct
     3001 gaacctcctc attgcccagg ccgacgcggg cgccatcgac ctcgtcgtca agggaagggc
     3061 ttcgggccag gcgcgtggct ggttctacgt cgggggaggc cagtacgaga gtgacggcgt
     3121 cggcgagccc ctggtgacga ccgccgaact catggccggg gcggcagcgg gtgccgagcg
     3181 gacctggacc ggcgtccccc cgggcgcggg cgaacggatc ggcatcgacc gcgacctaga
     3241 cggctacggc gatcgccacg agctctccgc cggctccaat ccggccgacc cgaactcgat
     3301 cccctccacc agcaccgtgg gcggtccggt cgacggcccc ggtggacggg tgccgacgct
     3361 cgcggcctcg ccgaatccgg ccggcccggc cggcacggag atctcgttca cgctggccga
     3421 cgcggccgag gtctcgctgg aaattttcga tctctcaggc cggcgcgtgg ccacgctcct
     3481 cgatggccgg cgcgagcagg ggacggtgac cgtccactgg gacggccgcg acgactcggg
     3541 gcggccgatg gcggggggcg tgtacttttc gcgccttgcg gccgccggtg cgagcctcac
     3601 acgaaagatc gtgctggcac cctgacgccg ggcaatcagc gc
//
LOCUS       DASVOD010000235         6147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_173426, whole genome shotgun sequence.
ACCESSION   DASVOD010000235 DASVOD010000000
VERSION     DASVOD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173426"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2313)
                     /locus_tag="VF720_04975"
     CDS             complement(<1..2313)
                     /locus_tag="VF720_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878737.1"
                     /translation="MPSFRTIGLCALLLTAAVSPAGAITADRLLEASGEAAADWFGHA
                     VVGGFDFNGDGYDDVAISAPFLSTAILDAGRVYIHFRGPGADGVADVTLTGLTVDDNL
                     GWSFSSGDFNNDGYDDLVVAALDCCAYKGIAYIFLGGAVPDNVADLTMTGAANDDLYG
                     YSVSGAGDLNGDGFDDWIIGAPQNDTGGSNAGRAYVYLGGSTLNSVIDLTLTGEAAGD
                     QFGGDLSGVGDINNDGYDDWAVMADRHDGNGTNAGRAYLYYGGSTLNSTVDLVVDGVL
                     SDYLGRVSGAGDMNGDGYDDWAVGAFVSGTSSSGVAHVLFGGAIPNAVPDITINGGSG
                     DSLGFALAGGGDVDGDGYDDLVVGVPGGDSEGEVQVFRGGPLTDARADLVLTGDESYD
                     RFGYAVALAGDENGDGASDLVVGAIYGGSGGKAYVYGLNGWRLVSPNGGEQWVTGQPA
                     VIRWRGRELTDVALSTDAGASWTTLVEDAGGALENEIVIDAPATTGDAAMVRLRIASL
                     AISPSSTDVSDATFRIVGPHEPPAAAYTQQATLVGAALGDAFGVAVAGAGDLNGDGFD
                     DIVTGANGNDAAGTSAGRVYIHFGGADFDTTADLVLSGILTGDSYGTAVDAAGDVNGD
                     GFDDLVVGSVSHDGTFVNGGAAFVYFGGSPMNGLADWTLLGTQTSESFGGFVAGAGDV
                     NGDGYDDIVVGAPGNDVVFTNAGAAYLYLGGSAPDAVADWIVYGAASSDGLGAVDGVG
                     DVNRDGYADLVVGASGNDAGGSNAGRAYVHF"
     gene            complement(2501..2809)
                     /locus_tag="VF720_04980"
     CDS             complement(2501..2809)
                     /locus_tag="VF720_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878738.1"
                     /translation="MSRISERSPWRWLAALSAISGLLVLLAIAAGGEASLGMAVPAST
                     MCVGGLLLFFRMSVDEEGWERIVHGPTARRFLAVAIVIAALIGLATVLVMMGLALKQG
                     "
     gene            complement(2953..3765)
                     /gene="msrA"
                     /locus_tag="VF720_04985"
     CDS             complement(2953..3765)
                     /gene="msrA"
                     /locus_tag="VF720_04985"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7878739.1"
                     /translation="MNRNSIVPAIAALVVLMGCGGAPKSQAQSDPKDVPLAADSTAPS
                     GRGVMQSLDGTEEAISLAPETDGAVGVPDLAPAPASKHPGMAMLPPAKKNEEIAIFAG
                     GCFWCMEKPFEKIKGVTSVISGYTGGHTLDPSYDLVNTHTTGHVEAVRVAFDTTVVSY
                     ARLLDFYWHNVDPTTEDRQFCDAGDSYVSRIYWRTDSQRAAAEASRIKVARWLKTPEP
                     IVTAILPARTFWPAEEYHQDYYRKNPVEYEAYRLACRRDQRLKQLWGDAAKH"
     gene            complement(3765..4307)
                     /gene="msrB"
                     /locus_tag="VF720_04990"
     CDS             complement(3765..4307)
                     /gene="msrB"
                     /locus_tag="VF720_04990"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX7878740.1"
                     /translation="MRYVTLPWILAALTLTFTVAACAAPPPATSKGSADMAIKAEDLK
                     PLNQPPEHWKPFLTDEAYHVMFEQGTERAFTCPLNEEHRKGTFVCAACHLPLFTSEQK
                     FDSGTGWPSFNAPLAGHFGFTKDTEIGYVRDEYHCIRCGGHLGHVFDDGPPPTGQRWC
                     TNGVAMIFVPEGQPLPELRS"
     gene            complement(4465..4887)
                     /locus_tag="VF720_04995"
     CDS             complement(4465..4887)
                     /locus_tag="VF720_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7878741.1"
                     /translation="MTYWDTSALVALYVGQVRSAEVRALAARPGRIVTWTVSEIEVRS
                     ALARLEREASVPADALRQAREDASRHWESFAVIGTVDAVKTRARRLLGVHALRAADAL
                     QLAAALTAASDDPTGHSLVTLDERLATAAGREGFTILP"
     gene            complement(4884..5222)
                     /locus_tag="VF720_05000"
     CDS             complement(4884..5222)
                     /locus_tag="VF720_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878742.1"
                     /translation="MSRSIKRLDKPVDSGYSSLMKRVSVTDLKNGLSHYLRLVKRGET
                     IEVMEHQVPIARLVRLEAGVDEDPQLERLVRDGIVTLGARDPGTVKLRPPIECPVDIV
                     RILLEERGGE"
     gene            complement(5219..>6147)
                     /locus_tag="VF720_05005"
     CDS             complement(5219..>6147)
                     /locus_tag="VF720_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7878743.1"
                     /translation="AGGHNILFVGPPGAGKTMLARRLPTLLPTLSLDEAMIVTRIHSV
                     AGRLPLGRPLVLERPFRAPHHTISDSGLIGGGPRALPGELSLAHGGVLFLDELPEFRR
                     NVLEALRQPLEEGFVRVSRAMLDVRYPADAMIVGALNPCPCGHWGDVRRRCLCSDREI
                     RAYRLRVSGPLLDRFDIHLELSPVPFDELASRDRGEPSLPIRERVAAAREIQIERFLG
                     EGTDIPYRPRLPVNARMTHEEMDRWCVTDDAANHLLRRAMERFGLSARAHRRILKVAR
                     TIADLVGSGTLAPVHVAEAVQYRCLDREGVGV"
BASE COUNT         1027 a         2141 c         2007 g          972 t
ORIGIN      
        1 gaagtgcacg taggcgcggc cggcgttgct gccgccggcg tcgttccccg aggcgcccac
       61 caccaggtcg gcgtagccgt cccggttcac gtcgccgact ccgtccacgg cccccagccc
      121 gtcggaggac gccgcgccat acacgatcca gtccgccacc gcgtcgggtg ctgatccgcc
      181 aaggtaaagg taggccgcgc cggcgttcgt gaagaccacg tcgttgcccg gcgcaccgac
      241 gacgatgtcg tcgtagccgt cgccgttcac gtcgccggcc ccggccacga atccgccgaa
      301 gctctcgctg gtctgcgtgc cgaggagcgt ccagtcggcc aggccgttca tgggggagcc
      361 gccgaagtag acgaacgccg cgccgccgtt cacgaacgtg ccgtcgtggc tcaccgagcc
      421 gaccaccagg tcgtcgaagc cgtcgccgtt cacgtcaccc gccgcatcca ccgcggtgcc
      481 gtacgaatcg cccgtcagga tgcccgacaa cacgaggtcg gccgtcgtgt cgaagtccgc
      541 cccgccgaaa tggatgtaga cccgcccggc gctcgtccct gcggcgtcat tcccgttggc
      601 tcccgtcacg atgtcgtcga aaccatcgcc gttcaggtcg cccgcgccgg ccaccgccac
      661 cccgaaggca tcgcccagcg cggcgccgac cagggtggcc tgctgcgtgt acgccgccgc
      721 cggcggctcg tgcggaccga cgatccggaa cgtggcgtcg ctcacgtccg tgctggaggg
      781 ggagatggcc agggaggcga tccggagccg gaccatggcg gcatcacccg tggtggccgg
      841 cgcatcgatg acgatctcgt tctcgagcgc gccaccggca tcctcgacca gcgtcgtcca
      901 gctggcgccg gcatccgtcg agagcgccac gtcggtcagc tcgcgtcccc gccagcggat
      961 cacggcgggt tggccggtga cccactgctc gccgccgttc ggcgacacga gccgccagcc
     1021 gttcaacccg tagacgtagg ccttgccgcc gctccctccg taaatcgccc cgaccacgag
     1081 atcggaggcg ccgtcgccgt tctcgtcgcc ggccagcgcc acggcgtacc cgaaccggtc
     1141 gtacgactcg tcgccggtca gcacgaggtc ggcgcgggca tcggtgagcg gtccaccacg
     1201 gaagacctgg acttctccct cggaatcacc gccgggaacg cccaccacca ggtcatcgta
     1261 accgtcgccg tcgacatccc cgccgccggc cagcgcgaat ccgagggagt cgccgctccc
     1321 gccgtttatg gtgatgtcgg gaacggcgtt ggggatggcc ccgccgaaca ggacatgcgc
     1381 gacgccgctc gagctggttc cgctcacgaa ggcgcccacg gcccagtcgt cgtagccgtc
     1441 gccgttcatg tcacccgccc cggagacccg acccaggtag tccgaaagga cgccgtccac
     1501 gacgaggtcc accgtggaat tcaacgtcga ccccccgtag tagagatagg cccgcccggc
     1561 gttcgtgccg ttgccgtcgt gccggtccgc catcacggcc cagtcgtcgt aaccatcgtt
     1621 attgatgtcc ccgacgccgg acaggtcgcc gccgaactgg tcgccggccg cctcgcccgt
     1681 aagggtgagg tcgatcaccg aattcagggt ggagccgccg agatacacgt aggcgcgtcc
     1741 ggcattactc ccacccgtgt cgttctgggg cgcgccgatg atccagtcat cgaagccatc
     1801 gccattcagg tctcccgcac ccgacacgga gtacccgtac aggtcgtcgt tggcggcccc
     1861 ggtcatcgtc aggtccgcaa cgttgtcggg cacggctccc cccaggaaga tgtaggcgat
     1921 ccccttgtac gcgcagcagt cgagcgccgc caccaccagg tcatcgtatc cgtcgttgtt
     1981 gaaatcgccc gaggagaagg accagccgag gttgtcgtcg accgtgaggc cggtcagcgt
     2041 cacgtcggcc actccgtcgg caccgggccc cctgaagtgg atgtacacgc ggccggcatc
     2101 gagaatggcc gtcgagagga atggggcgct gatggccacg tcgtcgtacc cgtcgccatt
     2161 gaagtcgaaa ccgccgacca ccgcgtgtcc gaaccagtcg gcggcagcct caccgctggc
     2221 ctcgaggagc ctgtcggccg tgatggcccc ggcaggggac acggcggcag tgagaagcag
     2281 ggcgcaaagg cctatggtgc gaaatgacgg catgatccct ccagattgga cgtcgacccc
     2341 gtcctcgccg gccggaagtc gctcttcgcc ggggttacac ggaatcggcc cgcgagggga
     2401 catgcggggg ggtgtggctt gcgggcgcgg caaggccagg ctgctcaagc gcatccgcgc
     2461 tggccgccac ccgggaggtg gcgggggaat gttcggtcgg ctatccctgc ttgagggcca
     2521 gtcccatcat gacgaggacg gtagccaggc cgatgagcgc cgcgatgacg atggctaccg
     2581 ccaggaagcg gcgggccgtc ggcccgtgaa caatccgctc ccatccttct tcatccacgg
     2641 acatccggaa gaagaggagc agaccgccga cgcacatcgt cgaagccggc accgccatcc
     2701 ccaggctcgc ttcgccgccc gccgcaatgg ccaggagaac gaggagtccg gaaatcgcgg
     2761 agagggccgc cagccatcgc catggggatc gttcgctgat ccgactcatg catgaccgat
     2821 cggcattttg cggaggcgct tgagcgccga gcgcggcaat tcgcacccgt ccgagcaatc
     2881 tgcagggcat ttccgtgcgg cctgaatgat atgggtgaaa tgccccgcac acgtgtcgtc
     2941 acacgaggac ggtcagtgct tcgccgcgtc tccccagagc tgcttcagcc gctggtcccg
     3001 ccggcaggcc aaccggtacg cctcgtactc gacggggttc ttcctgtagt aatcctggtg
     3061 gtattcctcg gctggccaga acgtccgggc ggggaggatg gcggtcacga tcggctccgg
     3121 cgtcttgagc cacctggcca ccttgatccg gctggcctcg gcggcggccc gctgcgagtc
     3181 ggtccgccag tagatgcgcg acacgtacga atcgcccgcg tcgcagaact ggcgatcctc
     3241 cgtcgtgggg tcgacgttgt gccagtagaa atcgaggagc cgcgcgtagc tcaccaccgt
     3301 cgtgtcgaac gccactcgca cggcctccac gtgtccggtg gtgtgggtgt tcaccaggtc
     3361 gtagctcggg tccagtgtgt gcccacctgt gtaccccgag atcaccgacg taaccccctt
     3421 gatcttctcg aacggctttt ccatgcacca gaagcagccg ccggcaaaga tggcgatctc
     3481 ctcgttcttc ttcgcggggg gcagcatggc catccccggg tgcttcgacg cgggagcagg
     3541 agcgaggtcg ggcaccccga ctgccccgtc cgtttccggc gcgagcgaga tggcctcctc
     3601 ggttccgtcg agcgactgca tcacgccgcg gcccgacgga gcggtggagt cggcggccag
     3661 cggcacgtcc ttcggatccg actgagcctg cgacttcggc gccccgccac accccatgag
     3721 cacaaccagg gcggcaatcg ccggaacgat cgagttcctg ttcatcagct cctcaattcg
     3781 ggcagcggtt ggccctcggg cacgaagatc atcgctacgc cattcgtgca ccaccgctgc
     3841 ccggtcggcg gcggcccgtc atcgaacacg tgcccgaggt gcccgccgca ccggatgcag
     3901 tggtactcat cccgcacgta cccgatctcg gtgtccttcg tgaagccgaa gtgcccggcc
     3961 agcggcgcgt tgaagctcgg ccatcccgtc ccgctgtcga acttctgttc cgacgtgaag
     4021 agcggcaggt ggcaggcggc acacacgaac gtccccttgc ggtgttcctc gttcaacggg
     4081 caggtaaacg cccgttcggt cccctgctcg aacatgacgt ggtacgcctc gtcggtcagg
     4141 aacggcttcc aatgctcggg gggctggttg aggggcttca ggtcttcggc cttgatggcc
     4201 atgtcggcac ttcctttcga cgtggcggga gggggcgcag cgcaggcggc cacggtgaac
     4261 gtcagggtca gggcggccag gatccaggga agggtgacgt atctcatgga ctggcaaatg
     4321 cctccgggga tgaaaaggtt ccatcgcttg ccgggtgggg ggagcctgat tcagctttgc
     4381 ggacatgccg gcccggtacc agccagtacg aggcggtgcc ggtattggat gcgccaatgg
     4441 accaaccgat tcccggcctt tcggctaggg aagaatcgta aatccttcgc gccccgcggc
     4501 cgttgccagc cgctcatcca gcgtcacgag cgagtgaccc gtcgggtcat cggacgccgc
     4561 ggtgagggcg gcagccagtt gcagcgcgtc ggcggcgcga agggcatgaa ccccgagaag
     4621 tcgccgcgcc cgagtcttga ccgcgtccac ggttccgatg acggcgaaag actcccagtg
     4681 gcgcgacgcg tcctcccttg cctgtcgcaa cgcgtccgcc ggtacggatg cctcccgctc
     4741 cagtcgagcc aacgcggatc ggacctcgat ctcgctgacc gtccaggtga cgattctgcc
     4801 gggccgggca gccagggcac gaacttcggc agatcgaacc tggcccacgt acagggcgac
     4861 gagcgccgat gtatcccagt aggtcattcc ccgcctcgtt cctcaagcag gatacggacg
     4921 atatccaccg gacactcgat cggagggcgc agcttgacgg tgcctgggtc gcgagctccc
     4981 agcgtgacga ttccgtcgcg aacgaggcgt tcgagttgag ggtcctcgtc cactccagcc
     5041 tccagtcgga ccagccgggc gatcggaacc tgatgttcca tgacctcgat ggtctcgcct
     5101 cgcttgacca atcgaaggta gtggctgagc ccgtttttca ggtcggtaac actgacacgc
     5161 ttcataaggc tagagtagcc agaatcaact ggcttatcaa gccttttaat gctcctgctc
     5221 acactcccac cccctcccga tccaggcacc ggtactgcac cgcttccgcg acatgcaccg
     5281 gcgcgagcgt gccggagccg accaggtcgg caatggtccg cgccactttc aggattcgcc
     5341 gatgcgcccg cgccgacaac ccgaaccgtt ccatggcccg ccggagcaga tgattcgccg
     5401 catcgtccgt gacgcaccaa cgatccatct cctcgtgcgt catgcgcgca ttcaccggca
     5461 agcgaggccg atacgggata tcagtgccct caccgagaaa ccgctcgatc tggatctccc
     5521 gcgccgccgc cacgcgttcc cggatcggca gcgacggttc tccccgatcg cgcgacgcga
     5581 gttcatcgaa cggcacgggc gacagttcga gatgaatgtc gaatcgatcc agcaacggcc
     5641 ccgacacgcg aagccgatag gcgcggatct cgcgatccga gcagaggcaa cggcgtcgca
     5701 cgtcgcccca gtggccgcac gggcaggggt tcagggcgcc cacgatcatg gcgtcggccg
     5761 gataacggac gtcgagcatg gcgcggctca cgcgaacgaa cccctcctcg agcggctggc
     5821 gcagcgcctc gagcacgttc cggcggaatt ccggaagctc gtcgaggaag agcacaccgc
     5881 cgtgggccag cgacaactcg cccggcagcg cgcgcggccc gccgccgatc agcccggagt
     5941 cggaaatcgt gtgatgcggc gcgcggaacg gacgttcgag caccagcggc cggccgaggg
     6001 ggaggcgtcc ggccaccgag tggatgcggg tgacgatcat cgcctcatcc agcgacaagg
     6061 tcggcaacag ggtggggaga cgtcgggcga gcatcgtctt gcccgcgccg gggggaccga
     6121 cgaagagaat gttgtggccg cccgcgg
//
LOCUS       DASVOD010000236         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_380905, whole genome shotgun sequence.
ACCESSION   DASVOD010000236 DASVOD010000000
VERSION     DASVOD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380905"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF720_05010"
     CDS             complement(<1..602)
                     /locus_tag="VF720_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878744.1"
                     /translation="MILKRNEGPNSRPRNRPRPDRTSPPWLRRLAKVGLALSVVLVSV
                     PLFPLQRRAETTHYQVGQVIDEEILAPFGFDILRGEEEIDRDRTDAVRSVAPIFTVDE
                     TRAEATLRRLTRFAIDVRRAANSTQLRKGERAEQIGRLGVPLTAESRRVLADPDTSAV
                     ILEEVNRLITQSYKVGIADQSEMASLNDRLKVKLRHGGDET"
     gene            complement(599..1564)
                     /locus_tag="VF720_05015"
     CDS             complement(599..1564)
                     /locus_tag="VF720_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7878745.1"
                     /translation="MKAQRIETIDLESIDPLNLFGERDSHLRWIEQRYDVEATLRGRK
                     LAVAGEDGPVASAATLIRELIGKVKRGENLTAGEVDFAANRIDGGENEPLDMPNGGDV
                     LHFERRTIYLKSPGQRSYMEAIRRNDIVFGIGPAGTGKTFLAVAMAVTALKDRQVERI
                     VLVRPAVEAGESLGFLPGSFEEKVDPYLRPLYDALRDLIGHEKVRRFMETGVIEIAPL
                     AYMRGRTLSRSFVILDEAQNTTVKQMKMFLTRLGPDSKAVITGDVTQIDLEKPESSGL
                     VQIQEILRGIRDIRFCYFEERDVVRHRLVREILKAFTAHESKDDA"
     gene            1990..>2518
                     /locus_tag="VF720_05020"
     CDS             1990..>2518
                     /locus_tag="VF720_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878746.1"
                     /translation="MSFEPFVRHAESWKYRSARIHAVVLLAGFLTAGALLAAGAPMAG
                     AAPPVVERPDGTGGGQVVVPEPWANAPLPPLLSVTPGDERWMTPFNEPGIAGHVRAMA
                     WYEGSLILGGNFNSAGGVAASAIVRYDATGWHPMGEGLSIDHPFFDTPIVFDLQVYRG
                     ELYVAGIFTRAGGVDA"
BASE COUNT          376 a          845 c          830 g          467 t
ORIGIN      
        1 gtttcatccc ccccgtgccg cagcttcacc ttcaaccggt cgttgagcga cgccatttcg
       61 gactggtcgg cgatccccac cttgtacgac tgggtgatca gccggttcac ttcctcgagg
      121 atgacggccg aggtatccgg gtcggccagc acccgccggc tctcggccgt gagcggcacg
      181 ccgagccggc cgatctgctc ggcccgctcc cccttgcgga gctgcgtgga attggccgcc
      241 cggcgcacgt cgatggcaaa gcgcgtgagc cggcgcagcg tggcctcggc ccgggtttcg
      301 tccacggtga agataggggc cacgctccgg accgcgtccg tgcggtcccg gtcgatctcc
      361 tcctcgcccc ggaggatgtc gaacccgaac ggcgccagga tctcctcgtc gatgacctgt
      421 cccacctggt agtgggtggt ctcggcccgg cgctggagcg ggaatagcgg aacgctgacg
      481 aggaccacgg aaagcgccag gcccaccttg gccagtcgcc ggagccaggg gggactggtc
      541 cggtccggtc tcggccggtt gcgcggcctc gaattcggtc cctcgttccg tttcaggatc
      601 atgcgtcgtc tttgctctcg tgggccgtga acgccttgag aatttcgcgc accagacggt
      661 gccgcaccac gtcgcgctcc tcgaagtagc agaagcggat gtcgcggatc ccgcggagga
      721 tttcctggat ctgcaccagc ccgctcgact ccggcttctc gaggtcgatc tgcgtcacgt
      781 cgccggtgat gaccgccttc gagtcgggcc cgaggcgcgt caggaacatc ttcatctgct
      841 tcaccgtggt gttctgcgcc tcgtccagga tgacgaacga gcgcgagagc gtgcggccgc
      901 gcatgtaggc cagcggcgcg atctcgatga cgccggtctc catgaagcgg cggaccttct
      961 catgtccgat caggtcgcgc agcgcgtcgt agagcggtcg gagataggga tcgaccttct
     1021 cctcgaagct cccggggagg aaccccaggc tctcgccggc ttccaccgcg gggcggacga
     1081 ggacgatccg ctcgacctgg cggtccttga gtgccgtgac ggccatggcc acggcgagga
     1141 acgtcttgcc ggttccggcc ggcccgatgc cgaagacgat gtcgttgcgt cggatggcct
     1201 ccatgtagga gcgctggccg ggcgacttga ggtagatggt gcgccgttcg aagtggagga
     1261 cgtcgccgcc gttcggcatg tccaggggct cgttctcccc tccgtcgatg cggttcgccg
     1321 cgaagtccac ttcgccggcc gtcaggtttt cgccgcgctt caccttgccg atgagctcgc
     1381 ggatgagcgt ggccgccgag gccaccggcc cgtcttcgcc ggccaccgcc agcttgcggc
     1441 cacgcagcgt ggcctccacg tcgtagcgtt gttcgatcca gcgaaggtga ctgtcccgtt
     1501 cgccgaagag gttgagtgga tcgatggatt cgaggtcgat cgtctcgatg cgctgcgcct
     1561 tcattggcct ccggttcggt tacccagaaa gcgacaggct ccgggcgccc cgccatggcg
     1621 gagaggcgcc ggagcccggc acgtcacttc aaaatggttt tcgtatcgct ggcggaaata
     1681 ggcccacccg gatagggaac gtggtgctcg tcgccggaac cctgtatttc ggtctgcggg
     1741 gcctcggact tcaggattgc ccgaaacgct agcgccgggg ggcgggggct gtcaacgggg
     1801 ctggaccctg attgaccata actacctatt tataaacaac atacagattt tagtaagatt
     1861 tttcgactat catgccgtgg cgagcgtcag ggccggattt ccagcgattg ctcccctgcc
     1921 gaccgaggac cataattcac gggccacggc cgccgattcg acagttccgt tccgccggga
     1981 gcccgaccga tgtcattcga gccgttcgtg aggcacgccg agtcttggaa atatcgttcg
     2041 gcccgaatcc acgcggttgt cctcctcgcg gggttcctga cggccggcgc gcttctggcg
     2101 gccggcgcac ccatggccgg cgccgcgccc cccgtggtcg aacgacccga cggcaccggc
     2161 ggcggacagg tggtcgttcc cgaaccctgg gccaacgccc cacttccccc tctcctcagc
     2221 gtgacacccg gcgacgaacg ctggatgacc cccttcaacg aaccgggaat cgccggccac
     2281 gtgcgcgcca tggcctggta cgagggaagc ctcattctcg gcgggaactt caactccgcg
     2341 gggggcgtgg cggcctcggc catcgttcga tacgacgcca ccggctggca cccgatgggc
     2401 gaggggctct ccatcgacca tccctttttc gacacgccga tcgtcttcga cctgcaggtg
     2461 tatcgcggtg agttgtacgt ggccggcatc ttcacgcggg ccggcggggt ggacgcct
//
LOCUS       DASVOD010000237         4542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_641768, whole genome shotgun sequence.
ACCESSION   DASVOD010000237 DASVOD010000000
VERSION     DASVOD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641768"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..926
                     /locus_tag="VF720_05025"
     CDS             45..926
                     /locus_tag="VF720_05025"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878747.1"
                     /translation="MIGQTVGHYRVLEFLGHGESGAVYRAEDLIRHRLVALKILPAPH
                     FREPATAERIESGLNAAARLDHPAIPRIYDLGKDDQFRYVAMQLVEGTSLDARLRKGP
                     LAPEEAVSIAWEVASALETAHAAGLPHGDLKPSDIVMTPGGIRVHGFGSTPPVPTQSA
                     SIKGDLWRLAALLYEMLTGRQPFGRDDDARDRDNDAALVLPGQLRRGVPKSLDHAVAR
                     ALSHESSERPASASAFQKLLQEAGTDLNALTLARAPLARRRRRHFGTLLPTLSAVALL
                     ILVWLLWNAMRGAVGGN"
     gene            complement(1235..2761)
                     /locus_tag="VF720_05030"
     CDS             complement(1235..2761)
                     /locus_tag="VF720_05030"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7878748.1"
                     /translation="MKRRDFLARTGGLLATTWGAGSVWWPLTRRALAGESSVTGNKVL
                     VMVVLQGGNDGLNTVVPYNDPAYVLKRPVLKLPSAQVLDLDMGTTGLHPSLAPLLPIW
                     NQGRLGIIRDVGYPYMNLSHFRATDIMFSGSSSEDVVSTGWMARWLETLYPGFPETLP
                     ADPMALQQGFSAGLVLNGNRGTTGVVVDNPTTFYNIVNNNYTGEFTDPVSQTPGGDEL
                     DYIRDIDQQSFQYAGVIQDAADVGINYVPYPNTRLASQLAIVAQLIDANMATPLYLTA
                     QTGYDTHGDQLATHAALLSDLAGALAAFVADLDAMGRAADVCVLTVSEFGRRPAENGS
                     AGTDHGTSAPWFLIGQGINGGLSGGSPNLAGLDASGNLPVQADYRSVYGTILTGWMGT
                     APSVVNDILFGSFPSLSFLDTTGVPEAPPVGVTHLFAPTPNPGTGQRVFRFQLAAGGG
                     ARLRVFDVAGRQVATLSEAYLPAGNHEVRWNPDGLAMGTYVVALDAGGERRTAKLVVR
                     "
     gene            complement(2771..4462)
                     /locus_tag="VF720_05035"
     CDS             complement(2771..4462)
                     /locus_tag="VF720_05035"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX7878749.1"
                     /translation="MNRRCLAWCLAVALLLLPGAGSGRAEERATSASPGSAPTAPDAA
                     TAARIASFEAMIDARREVPALSTLSVRPGTDGDPHPGGAVAAYGWRQHDGPLTIEDAA
                     HLLNRALVGPRFEEFQWAVARGLPGTVHDLLQDPPTPAAPGAWATEPVPNQSTWTQAQ
                     KDSLDAVYRERQRLLRMWWTRTIATEPASLRETMTLFWHDHFATRMAVVYTPQSMYIQ
                     NALLRQHALGNFRTLARAICTDPAMLVMLDGRLNRKQSPNENFSREMLELFTMGEGSG
                     YTQADVSNAARACTGWQTDGTTSYLTASRYDTGTKTILGQTGPWGMDDFVRIIFEQDA
                     TAEYLCGKLYRWFVDDEPAASDIHELAVTLRANDYEIRPVLEKIFLSEHLVSARLRGS
                     IVRDGVDLYAGQARRWHLVGFDPMITSPQWQRNWITNQMNTYGQILLDPPDVSGWPGH
                     RNWINSNSLPNRKRYSVMIVDGRSSQNQPFGFQLDVNVETRRFRDPNDVNLLVDDLAL
                     LCFGQKPTQLVHDQLVAELLQGQLPYNWSIDDPNAVPRLQDLYRLAMRLPDYQLK"
BASE COUNT          719 a         1520 c         1565 g          738 t
ORIGIN      
        1 ggattccatg aggccgtgaa ttatagccaa cccggagtga ttcgatgatc gggcagactg
       61 tcggccatta ccgcgtcctg gaattcctgg ggcacggcga atcgggcgcc gtgtaccgcg
      121 cggaagacct gatccgtcat cgcctggtgg ccctcaagat cctcccggcc ccccatttcc
      181 gtgaacccgc cacggccgag cggatcgaat cgggactcaa tgccgccgcc cggctcgatc
      241 accccgccat cccccgcatc tacgatctcg gcaaggacga ccagttccgt tacgtggcga
      301 tgcagcttgt cgaggggaca tcgctcgacg cccgccttcg caaggggccg ctggcgccgg
      361 aggaggccgt cagcatcgcc tgggaggtgg cctccgccct ggaaaccgcc cacgccgccg
      421 gccttcccca cggcgacctg aaaccgtccg acatcgtgat gacgcccggc ggcatccgcg
      481 tgcacggctt cggctccacg cctcccgtgc cgacccagtc cgccagcatc aagggagacc
      541 tgtggcgact ggccgcactc ctctacgaga tgctcaccgg ccgccagccc ttcggccgcg
      601 acgacgacgc ccgcgaccgt gacaacgacg ccgcgctggt cctccccggc cagctccgcc
      661 gcggcgtgcc gaaaagcctc gaccacgccg tcgcgcgggc cctgagccac gaatcgtccg
      721 aacgccccgc ctcagcttcc gccttccaga aactgctcca ggaggccggc accgatctga
      781 acgcgctcac gctggcccgc gcgccgctgg cgcggcgccg acgccgccac ttcggcaccc
      841 tgttgccgac gctctctgcc gtggcgctcc tcatcctggt gtggttgctc tggaacgcga
      901 tgcggggggc ggtgggcggg aactgatcgg acggacggag ccgggaggag ggagccgttc
      961 gaggacgctc gctacgccgg ccaggataaa gcgaagccgg cgctcatcat ttctgactac
     1021 catggcgtca atttgccaag agaattgccg ccatcatgta caagatatgt aacccattac
     1081 aatttaatcg gttatagtca aataagtcgc ccgaagggcg acctcaccgg gcttttcgac
     1141 atcgttcggc gccggacgct cgcgccagat cgcgtgcagg aggtcgggcg gcacggggct
     1201 cctccgcgga aacgggacag aagcaggtta caccctatcg caccaccagc ttggccgtcc
     1261 gccgttcccc acccgcgtcg agcgccacga cataagtgcc catcgcgagt ccgtccggat
     1321 tccagcgcac ctcgtggttg cccgccggca gatacgcctc ggacagcgtg gccacctggc
     1381 ggccggccac gtcgaacaca cgcaagcgcg cgccacctcc tgcagccagc tggaagcgga
     1441 agacccgctg tcccgtgccc ggattgggcg tcggggcgaa gaggtgcgtg acgcccaccg
     1501 gcggcgcctc ggggacgccg gtcgtgtcga ggaatgacaa cgacggaaac gagccgaaaa
     1561 ggatgtcgtt caccacggag ggcgccgtgc ccatccagcc ggtcaggatc gtgccgtaca
     1621 cggaacgata gtcggcctgg accgggaggt tgccgctcgc gtcgagtccg gccaggttgg
     1681 gcgagccgcc gctcaggcca ccattgattc cctggccgat gaggaaccag ggggccgacg
     1741 tgccgtggtc ggtgccggcg ctgccgttct cggcggggcg gcggccgaat tcggagacgg
     1801 tgaggacgca gacgtcggcc gcgcgcccca tggcgtcgag gtcggccacg aaggcggcca
     1861 gcgcgccggc caggtcggag aggagcgccg cgtgcgtcgc gagctggtcg ccgtgggtgt
     1921 catatcccgt ctgggccgtg agatagagcg gcgtggccat gttcgcgtcg atgagctgcg
     1981 ccacgatcgc cagctggctg gccagtctcg tgttgggata gggaacgtag ttgatgccga
     2041 catccgcggc atcctggatg acaccggcgt actggaacga ctgctggtcg atgtcgcgga
     2101 tgtagtcgag ctcgtccccg cctggagtct gcgagacggg atcggtgaac tcgcccgtgt
     2161 agttgttgtt gacgatgttg tagaacgtgg tcgggttgtc gaccaccacg ccggtcgtcc
     2221 cgcgattgcc gttgagcacg aggcccgccg agaacccctg ctgcaacgcc atcgggtcgg
     2281 ccggcagcgt ttcgggaaag ccgggataga gcgtttcgag ccagcgggcc atccagccgg
     2341 ttgaaaccac gtcctcgctg ctcgaacccg agaacatgat gtcggtggcc cggaagtggc
     2401 tcaggttcat gtaggggtaa ccgacgtcgc ggatgatgcc gaggcgcccc tggttccaga
     2461 tgggaaggag cggcgccagc gatgggtgga ggccggtggt tcccatgtcg aggtcgagca
     2521 cctgggccga ggggagcttc aacacgggac gcttgagcac gtaggccggg tcgttgtacg
     2581 gcacgacggt gttgaggccg tcgtttcccc cctgcagcac caccatcacc agcaccttgt
     2641 tgccggtcac gctcgattca ccggccagcg cccgccttgt caacggccac cagacgctcc
     2701 ccgcgcccca ggtggtggcg aggagcccgc cggtacgggc caggaaatca cgccgcttca
     2761 tcgcccctcc tcatttcagc tggtagtcgg gcaggcgcat ggccagccgg tagaggtcct
     2821 ggagccgcgg cacggcgttc gggtcgtcga tcgaccagtt gtacggcagc tgcccctgca
     2881 ggagctcggc caccagctgg tcatgcacca gttgggtcgg cttctggccg aagcagagga
     2941 gcgccaggtc gtcgaccagg aggttgacgt cgttggggtc gcggaagcgg cgcgtctcga
     3001 cgttcacgtc gagctggaag ccgaacggct ggttctgcga gctcctgccg tccacgatca
     3061 tcacgctgta gcgcttgcgg ttcggcagcg agttggagtt gatccagttg cgatgccccg
     3121 gccaccccga cacgtcgggc ggatcgagca ggatctggcc gtaggtgttc atctggttgg
     3181 tgatccagtt ccgctgccac tgcggggagg tgatcatcgg gtcgaacccc accaggtgcc
     3241 agcggcgcgc ctgtcccgcg tacagatcca cgccgtcccg cacgatcgag ccccggaggc
     3301 gggcgctgac caggtgttcg ctgaggaaga tcttctcgag gacgggacgg atttcgtagt
     3361 cgttcgcgcg gagcgtgacg gccagctcgt ggatgtcgga cgccgccggt tcgtcatcca
     3421 cgaaccagcg gtagagcttg ccgcacaggt attcggccgt ggcgtcctgc tcgaagatga
     3481 tccggacgaa atcgtccatc ccccacgggc cggtctggcc caggatcgtc ttcgtgccgg
     3541 tgtcgtagcg gctggcggtg aggtagctgg tggtgccgtc ggtctgccag ccggtgcagg
     3601 cgcgcgcggc gttgctcacg tcggcctgcg tgtaccccga tccctccccc atcgtgaaga
     3661 gctcgagcat ctcccgggaa aagttctcgt tgggcgactg cttccggttc aaccggccgt
     3721 cgagcatcac cagcatggcc ggatcggtgc agatggcccg ggccagcgtc cggaaattgc
     3781 cgagggcgtg ctggcggagg agcgcattct ggatgtacat gctctgcggg gtgtacacca
     3841 ccgccatccg cgtggcgaag tgatcgtgcc agaagagcgt catggtttcg cgcaggctgg
     3901 ccggctcggt ggcgatggtc cgcgtccacc acatgcgcag cagccgctgc cgctcgcgat
     3961 aaacggcatc gagcgaatcc ttctgggcct gtgtccaggt ggactggttg ggcaccggct
     4021 cggtggccca cgcgccgggc gccgccggtg tcggcgggtc ctgcagcagg tcgtgcaccg
     4081 ttccgggaag cccgcgcgcg acggcccact ggaactcctc gaagcgcggc cctacgaggg
     4141 cgcggttcaa caggtgcgcg gcatcctcga tcgtgagagg cccgtcatgc tgccgccagc
     4201 cgtacgccgc caccgcgccg ccggggtggg gatcgccgtc ggtgcccggc cgcacgctca
     4261 gggtggagag ggccggcacc tcgcgacggg cgtcgatcat cgcctcgaaa ctggcgatgc
     4321 gggccgccgt ggcggcatcg ggggcggtcg gtgccgatcc cggtgacgcc gatgttgccc
     4381 gttcctccgc acgcccgctt cccgcgccgg gcaacagcag aagcgcgacg gcaaggcacc
     4441 acgcgaggca gcggcgattc atcgggagtg gccctccgcg gcgggacgaa cgggatcgat
     4501 ggcggccggg cgttatcgtg ccgacttcgc gcccgtcagt cc
//
LOCUS       DASVOD010000238         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_693836, whole genome shotgun sequence.
ACCESSION   DASVOD010000238 DASVOD010000000
VERSION     DASVOD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693836"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VF720_05040"
     CDS             complement(<1..520)
                     /locus_tag="VF720_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878750.1"
                     /translation="MSFTRWIRAVALVVLLAPTAVMADKGLIGVWEAIVEGEPYILVL
                     DDNGTGRLLDQPIRWDTADDVFQVMVDGNSMAYRYALSGDQLVFSDGGTAPITFTRRG
                     SDAAGDNAATGSAAASGATGGSLVGKWRDSNGAVIELKGDGTGANAAGTFHWQAADGY
                     FYLVESNLVLRYE"
     gene            complement(550..1926)
                     /locus_tag="VF720_05045"
     CDS             complement(550..1926)
                     /locus_tag="VF720_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7878751.1"
                     /translation="MGARFFPDLLASKNGRLAAFFLLYMTEGIPLGFTATAIGTQMRR
                     QGVSPEMIGAFVGSLYLPWAFKWIAGPFVDTFASDRFGRRRLWILLMQLGMVATLCVA
                     YPVNFVTNLSLFTIIIAVHNAFAATMDVAIDSLACNVLPESERGVANGFMFGGASVGQ
                     TVGGSMVLFLTSIMPFQATYFFVMGVIMLVTVFIVLPLQEKSEPRPAVPGSKLSAVGF
                     QLWRFAVDAFRAFTGTRAAFVGLIAALLPLGAYALSLSLQSTLAVELGLNDNQVATLQ
                     LASTLIFAVTCIAGGWLSDRFGRRTTLALFGGLTVVPTLYLAWSLQSHHWIHSIPANA
                     ADRPTVPATLVTAFWIASIAYMVFQGLYYGIRTALFMDITTPAVAATQFTAYMAMSNL
                     CISYTSTWQGFAITKYGYPITLVLDSAAGFLAIALLPFMNPVKRTAVTEPFAAPRPGD
                     AIPEGMGP"
     gene            2071..2982
                     /locus_tag="VF720_05050"
     CDS             2071..2982
                     /locus_tag="VF720_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878752.1"
                     /translation="MKRFIVVTIGATLALLLSLVALPGSSRAQVVNPGDLPLKPDSTE
                     VHVEHARTWEMPEVIVFGKSHKLREEQRIGPYRQPRWTATRRFTTTRTYVIPPGKAEF
                     EFWVRPTFKNDGTVEMRSLWELEFGLPHRFQLDLYLRTDQVLDPGGEPASHGQQIEVR
                     WALADWGKIWGNPTLYLEWVNLYDSPDKIEPKLLLGGQLAEGWQAGANLVGEFEMGGE
                     REYEYEFTGGLGHPIIDSQFSIGLETRLAWADVASDRGNFSQNFRLGPSIQFRPLNRM
                     TINVAALAGLTEDSDDAQMFVNTGWEF"
     gene            3070..>3346
                     /locus_tag="VF720_05055"
     CDS             3070..>3346
                     /locus_tag="VF720_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878753.1"
                     /translation="MSLIGRFQSLPVTRLFPAALAAVLLAWPAKAFSTDDGVWTSLAL
                     PDPLVLGPGASLVYDSSRHRALTRTGGVLYALDLADPVAWEAITPIGP"
BASE COUNT          622 a         1045 c         1142 g          537 t
ORIGIN      
        1 gttcgtagcg cagcacgagg ttcgactcga ccaggtagaa gtagccatcg gcggcctgcc
       61 agtgaaatgt tccggccgcg ttggcgccgg tgccgtcccc cttgagctcg atgacggctc
      121 cgttggaatc acgccacttg cccacgagcg atccgccggt ggccccgctc gcggccgcgc
      181 tgccggtggc ggcgttatcg cccgcggcat cggacccacg acgcgtgaac gtgattgggg
      241 ccgtgccgcc atccgaaaag acgagctggt cgccgctgag cgcgtagcgg tacgccatgg
      301 agttgccgtc caccatcacc tggaagacgt cgtcggccgt gtcccagcgg atgggctggt
      361 cgagcaggcg gccggtgccg ttgtcgtcga ggaccaggat gtacggctca ccttcgacga
      421 tagcttccca gacgccgatc agccccttgt cagccatgac ggcggtgggg gcgaggagaa
      481 cgacgagtgc cacggcgcgg atccatcttg tgaacgacac gacattgctc cttcattcga
      541 atggggcggc tagggcccca tgccttcggg aatggcatcg ccggggcgcg gcgcggcgaa
      601 cggttcggta acggcggttc gtttcaccgg gttcatgaac ggcaacagcg cgatggccag
      661 gaagccggcc gcggagtcga gcacgagcgt gatcggataa ccgtacttcg tgatggcaaa
      721 accctgccag gtagaggtgt acgagatgca gaggttgctc atcgccatgt aggcggtgaa
      781 ctgcgtggcg gccaccgccg gggtggtgat gtccatgaag agcgcggtgc ggatgccgta
      841 atagaggccc tggaacacca tgtaggcgat gctggcgatc cagaaggcgg tgaccagggt
      901 ggccggcacc gtcgggcggt ccgcggcatt cgccgggatg gagtggatcc agtggtggga
      961 ctggagcgac caggccagat agagcgtcgg aaccacggtc agtccgccga acagcgccag
     1021 cgtggtgcgc cggccgaatc gatcggaaag ccagcccccc gcgatgcagg tgaccgcgaa
     1081 aatgagcgtg gaggcgagtt gcagtgtggc cacctggttg tcgttcaacc cgagttccac
     1141 tgccagcgtg gactggagcg aaaggctcag ggcataggcg ccgagcggaa ggagcgccgc
     1201 gatcaggccg acaaatgcgg cccgggtgcc ggtgaaggcc cggaaggcat cgacggcgaa
     1261 gcgccacaac tggaagccga cggccgaaag tttcgagccg ggtaccgccg gtcgcggctc
     1321 gctcttctcc tgcaacggca ggacaatgaa gaccgtgacc agcatgatga cgcccatgac
     1381 gaagaagtag gtggcctgga acggcatgat gctcgtcagg aagaggacca tcgagccgcc
     1441 caccgtctgg cccaccgagg cgccgccgaa catgaagccg ttggccacac cgcgttccga
     1501 ttcgggaagg acgttgcacg ccagcgagtc gatggccacg tccatggtgg cggcgaatgc
     1561 gttgtgcacg gcgatgatga tcgtgaagag cgacaggttg gtgacgaagt tgaccggata
     1621 cgccacgcac agcgtggcca ccatcccgag ctgcatcagc aggatccaga ggcgccgtcg
     1681 accgaagcga tcgctggcaa aggtgtcgac gaacggcccg gcgatccact tgaacgccca
     1741 cggcaggtag agcgagccga cgaaggcgcc gatcatctcc gggcttaccc cctggcggcg
     1801 catctgggtg ccgatggcgg tggcggtaaa gccgagcggg atcccctcgg tcatatagag
     1861 gaggaagaag gcggcgaggc ggccgttctt gctggccaac aggtccggga agaagcgtgc
     1921 gcccatgagt gatgactccg gagagggttt cggcaagccc gaaaggggga tggtatcgcg
     1981 ctcgggagga tcgcgaaagc tggcgtttac aagagggatt gatatagtgc cgcgaccttc
     2041 cgacgcaccg gtccccaacc tcgaggccgc atgaagcgat tcatcgtcgt aacgatcggc
     2101 gccaccctgg cgctgctgct ctcgctggtt gcccttcccg gatcctcacg ggcccaggtc
     2161 gtaaaccccg gcgatctccc gctcaagccc gattccacgg aggtgcacgt cgagcatgcc
     2221 cgcacctggg aaatgcccga ggtgatcgtg ttcggcaagt cgcacaagct gcgcgaggag
     2281 cagcggatcg ggccctaccg ccagccgcgc tggacggcca cgcgtcggtt caccaccacg
     2341 cgtacgtacg tcatcccgcc cggcaaggcc gagttcgaat tctgggtgcg ccccaccttc
     2401 aagaatgacg gcaccgtcga gatgcgcagc ctctgggagc tcgagttcgg tttgccgcac
     2461 cgtttccagc tcgacctcta cctgcgcacc gaccaggtgc tcgaccccgg cggcgagccc
     2521 gccagccacg gccagcagat cgaggtgcgc tgggccctgg ccgactgggg aaagatctgg
     2581 ggcaatccca cgttgtacct ggagtgggtc aacctgtacg acagccccga caagatcgaa
     2641 cccaagctcc tcctcggcgg gcagctggcc gaggggtggc aggccggcgc caacctggtc
     2701 ggcgaattcg aaatgggggg cgagcgcgag tacgagtacg aattcaccgg cggcctcggg
     2761 caccccatca tcgacagcca gttctcgatc ggcctcgaga cacgcctcgc ctgggccgat
     2821 gtggcgagcg accgcggcaa cttctcgcag aactttcggc tggggccgag catccagttc
     2881 cgtccgttga atcgcatgac gatcaacgtg gcagcgctgg cggggctcac cgaggattcg
     2941 gatgacgcgc agatgttcgt gaatacgggg tgggagttct agggggaagt cgccgaaatc
     3001 gccattcacg gctatcttat tgggtactcc gaaacacagt tggcggtgtt cgtcaccacg
     3061 gagggcccga tgagcctcat tggccggttt cagagcctgc ccgtcacacg cttgttcccc
     3121 gcagccctcg ccgccgtgct cctcgcctgg ccggcaaagg ccttttccac ggatgacggg
     3181 gtgtggacat cattggctct gccggatccc ctggtgctcg gtcccggggc ctcactggtc
     3241 tacgactcgt cccgccatcg tgccctgacg cggactggcg gggtcctgta cgccctcgac
     3301 cttgcagacc ccgtcgcctg ggaagccatc acaccgatag ggccag
//
LOCUS       DASVOD010000239         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_486319, whole genome shotgun sequence.
ACCESSION   DASVOD010000239 DASVOD010000000
VERSION     DASVOD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486319"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1970)
                     /locus_tag="VF720_05060"
     CDS             complement(159..1970)
                     /locus_tag="VF720_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878754.1"
                     /translation="MKSASLRLSPGYLRPRILASWMSLAVLLPALALLGCDGQGPVAR
                     ATGYPDSLGPNITRLLIVDFGDNRLAPPGMLGIGSRTRHAVCGWSSGMIREVVRRDTT
                     DKDLADFVEGEDATFVLAVRSPDHPYEVVLTLGDPETARGPMSVSTRDVVVASGIRTK
                     PGQYKQVRFRADAPDRRITFRVRGGRCELFALTSLAVYGPLGSKMPSLAQTGPRDARP
                     FVPRPDSLATLGPKSDPRRILGTFADFLVAQEPVEGGFSYTGIWYQNAYPVRTLLAAG
                     RILDRPELVQEAFRVLDRFVDEQHADGRWTSSYFGRPECAGRVVADASSANFADIGSM
                     AHALAVGAGMADGDRRARYIEAARRYADEVVLPDQMADGAFPNRLYAGKDFPHPYSVA
                     TATQASSLAALAIVTGEARYRNAAEKAGQWLAERMLPSGFIVFHPHDADQPRLIRARD
                     FGDSFYLVEGLIWVHRAAADAATRDSVGAALRRHLWGPAGLKASTIHGYWWEPPGVWN
                     ASKTGGLLEAVAWAREDSPGAEVDRWIQLSQAWLADPLLRQRIGATSLLFMPNGEFSL
                     VATGFAGVGVASVIDFNAIFPVPRVTETPMPARVGTP"
BASE COUNT          368 a          693 c          690 g          301 t
ORIGIN      
        1 gagatgccga cgatcgccgc ggcagcaaat agatagacga ggccgctcct ggcgactcgg
       61 gtcaactcgg aactcctttc gacggttcac atgggcctcc accgccagag gttgcctccc
      121 tcacttgcat ggaaggtagc agagcttttt gaaaagtgtc aaggagtgcc aactcttgct
      181 ggcatcggag tttcggtcac tcgtggcacg ggaaagatcg cattgaaatc gatcaccgag
      241 gccaccccca cgcctgcgaa tccggtcgcc accagactga attccccgtt cggcatgaac
      301 agcaacgaag tagcgccgat gcgttgacgg agaagcgggt cggcgagcca cgcctgggaa
      361 agttgtatcc agcgatccac ctcggccccc ggagagtctt cccgcgccca cgccaccgcc
      421 tcgaggagac cccctgtctt ggaggcgttc cagaccccgg ggggctccca ccagtagccg
      481 tggatcgtgc tcgccttgag ccccgccggc ccccacaggt ggcgcctcag agccgccccc
      541 accgagtcgc gggtggccgc gtcggctgcg gcccgatgca cccagatcaa cccctcgacc
      601 aggtagaaac tgtctccgaa atcccgcgcg cggatcagtc ggggctgatc cgcgtcgtgg
      661 gggtggaaga cgatgaagcc cgacgggagc atccgctccg ccagccactg cccggccttc
      721 tcggccgcat tccggtaccg ggcttccccg gtgacgatgg ccagtgcggc caggctcgaa
      781 gcctgggtgg ccgttgccac gctgtaggga tgcggaaagt ccttcccggc gtagaggcga
      841 ttcgggaagg cgccgtccgc catctggtcg ggcagcacca cctcgtccgc ataccgcctg
      901 gctgcctcga tgtagcgcgc acgccgatcg ccgtcggcca tgccggctcc caccgccagc
      961 gcgtgcgcca tcgagccgat gtcggcgaaa ttggccgagg aggcgtcggc caccacgcgg
     1021 ccggcgcatt cgggccggcc gaaatagctg gaggtccatc ggccgtcggc atgctgctcg
     1081 tccacgaagc ggtcgagcac gcgaaacgct tcctggacga gttcgggacg gtccaggatg
     1141 cgcccggctg cgagcagcgt ccgcaccggg taggcattct ggtaccagat cccggtgtag
     1201 gaaaagcccc cctcgacagg ctcctgcgcc acgaggaagt ccgcgaacgt tccgaggatg
     1261 cgccgcggat ccgacttcgg tccgagcgtc gcgagcgagt cgggccgggg gacgaaggga
     1321 cgcgcgtcgc gcgggccggt ctgggccagc gaaggcatct tcgagccgag ggggccgtac
     1381 accgccagcg aggtcagagc aaagagttcg caccggccgc cgcggacacg aaacgtgatc
     1441 cgccggtccg gagcgtcggc ccggaaccgc acctgtttgt actggccggg cttcgtccgg
     1501 atcccggagg ctacgaccac gtcacgcgtg gagacgctca tggggccccg ggcggtttcc
     1561 gggtcgccaa gcgtcagcac cacttcatag gggtggtccg gcgatcggac cgccagcacg
     1621 aacgtggcgt cctccccttc cacgaagtcg gccaggtcct tgtccgtggt gtcgcggcgc
     1681 accacctcgc gaatcatccc gctcgaccag ccgcaaaccg cgtgacgggt tcgcgagccg
     1741 atgcccagca tcccgggcgg cgcgagccgg ttgtcgccga agtccacgat gagcaggcgg
     1801 gtgatgttcg gtccgagcga atcagggtaa cccgtggcgc gtgccaccgg tccctgccca
     1861 tcacagccca ggagcgccag ggccgggaga agcacggcca gtgacatcca cgaggcaagg
     1921 atgcgggggc ggaggtagcc ggggctcaac cgaagcgatg ctgatttcat gatccgggga
     1981 tcatgcccgg tccggcggac atctcccacg gcaaatgcaa aaaaatggga caaaaaaaac
     2041 cgggggcggg gg
//
LOCUS       DASVOD010000240         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_328906, whole genome shotgun sequence.
ACCESSION   DASVOD010000240 DASVOD010000000
VERSION     DASVOD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328906"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VF720_05065"
     CDS             <1..717
                     /locus_tag="VF720_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878755.1"
                     /translation="EVVMPIERPILFRLVCCVLFVAAAFAIPRPASAGTADDLPRIAL
                     HATSLAFKNPCGYRPNTEALTTHQPTLPFEARNVFVLVCNGPTAGISGLQFGVDYPGV
                     FNREGDPEADMVVYAFASCAYLSFDAPGWPAPGTGTLLAWNYDECQLLDDTGGGTFAN
                     AGYFYLSVYRPGTLRITPRPSDGRASLVDCSNVSYNITNVLDGGLLGSVGFGPDAGYN
                     PCAASVSVTPTTWSAIKRLD"
     gene            810..1097
                     /gene="xseB"
                     /locus_tag="VF720_05070"
     CDS             810..1097
                     /gene="xseB"
                     /locus_tag="VF720_05070"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX7878756.1"
                     /translation="MPPKAATKPPEGADAPSFEAALTRLEEIVNRLERGDITLDESLA
                     VFREGSGLVKLCLTRLSEAEATVEELMAGEGGAPTTVPGRPGLDGADDETA"
     gene            1081..2016
                     /locus_tag="VF720_05075"
     CDS             1081..2016
                     /locus_tag="VF720_05075"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEX7878757.1"
                     /translation="MTKRPDDTAEPMAVFAEFLETTSREVDDVMERLMPPVASMPKVI
                     HRAMRYSLFAGGKRIRPALVVLGYRASGGGGKVAHTAGAAIELIHTFSLIHDDLPCMD
                     DDDYRRGKLSNHKVFGEGVAVLAGDALHALGFDVLAGLRKFKGVSAETVLDVMDEVGR
                     AIGTDGMIGGQVMDLEWEGAKPSRSKVDYIHRRKTAALLGSSLVVGGILAGADKPTIK
                     ALRAYGEPFGLAFQIVDDILNVQQAGRALGKKPGGDERLGKMTWPAVHGLPRSQADVT
                     RLLDEASRAAPKFGPWMDHFLGLADYISVRRQARR"
     gene            2013..2462
                     /locus_tag="VF720_05080"
     CDS             2013..2462
                     /locus_tag="VF720_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEX7878758.1"
                     /translation="MREFIAARPWIIALMAGSLVQLMKFAADWGRHKRPNFRVLTSTG
                     GMPSSHSAGVCALTTAIYVTTGGRSALFSVALYFSLIVMYDATGLRRSAGLQAAILNR
                     IVDEQFIHHRKAGEGRLKELLGHTPLEVLVGAILGILFALTWYPSGG"
     gene            2483..>4280
                     /gene="dxs"
                     /locus_tag="VF720_05085"
     CDS             2483..>4280
                     /gene="dxs"
                     /locus_tag="VF720_05085"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEX7878759.1"
                     /translation="MGRYLDMVDSPRDLKKLSPEQLKTLADEVREEIIHVISQTGGHL
                     GASLGTVELTVALLHVFDSPIDKIVWDVGHQAYPHKLLTGRKQRFPTIRQYGGLSGFL
                     RRDESEYDAFGAGHASTAISAALGMAAARDQQGGTNKVVAVIGDGGLTGGMAFEALNN
                     AGDLKSDIIVVVNDNEMSISPNVGAMTKYLTRLTAAPIYRRFETDVYDLLGKIPAVGH
                     PAQELAGRVKESLTNLVVPTVFFEELGFKYFGPIDGHNLDGLVDTMKHVKEIGGPIVL
                     HVLTRKGKGYKPAEADRLALHGVTQFDKTTGAMKKKEAKAPTYSQIFGEALTEIANRD
                     PKVVAISAAMIEGTGLRIFEKSHPTKIYDVGIAEQHAVTFAAGMATEGMKPVCAIYST
                     FLQRAYDQIVHDVCLQNLDVTFVLDRAGLAGDDGATHHGVFDMAWLRTVPEMVIMAPK
                     DECEMRDMLYTAIEHPGPAALRIPRGEGSGARMNKDFTKLPIGEGEILSDGADVTIIG
                     IGAMVEQAVRVAEELGAQGIRAGVVNARFVKPLDTQLILERARKTGRLVVIEEHSPHG
                     GFGAAVLECLCDAGLSNIPVLHAALPDRFVHHG"
BASE COUNT          728 a         1446 c         1421 g          685 t
ORIGIN      
        1 gaggtcgtca tgcccattga gcgccccatc ctgttccggc ttgtctgttg cgtccttttc
       61 gtcgctgccg cgttcgccat cccacgcccc gcgagcgccg gtaccgcgga tgatctcccg
      121 cgcatcgccc tgcacgccac gagcctggcg ttcaagaacc cctgtgggta tcgcccgaat
      181 accgaggcat tgacgaccca ccagcccacg ttgcctttcg aggccaggaa cgtcttcgtc
      241 ctggtctgca acgggccgac cgccggcatt tccggcttgc agttcggggt cgattatccg
      301 ggcgttttca accgggaagg ggatccggag gcggacatgg tcgtctacgc gttcgcaagt
      361 tgtgcctacc tctccttcga tgcgccgggg tggccggccc ccggtaccgg cacgctcctc
      421 gcgtggaatt acgacgaatg tcagcttctc gacgacaccg gcggcggcac gttcgcgaat
      481 gccggctact tctacctgtc ggtgtatcgt cccggcacgc tgcgcatcac cccaagaccc
      541 tcggacggac gggccagcct ggtcgattgc tccaacgtct cctacaacat cacgaatgtg
      601 ctcgacggcg ggctgctggg aagcgtgggc ttcgggcccg acgcgggtta caacccgtgc
      661 gcggcctcgg tgagcgtgac gccgacgacg tggagcgcca tcaagcggct cgactgacgg
      721 cgccggagcg gcgattcaat ccccgccttg gtcgtcctcc gccgacgatg atagaatttc
      781 cgcgccgaac gacccccgga gagattgcca tgccaccaaa agccgccacg aagccccctg
      841 aaggcgccga tgcaccgtcg ttcgaagccg cgctgacccg cctggaagag attgtgaacc
      901 ggctcgagcg gggtgatatc acgctcgatg agtcgctggc ggtgttccgg gaggggagcg
      961 ggctcgtcaa gctgtgcctc acccggttgt ccgaggccga ggccacagtc gaagaattga
     1021 tggcgggtga gggtggcgcg ccgaccacgg tgccgggccg cccgggactc gacggagccg
     1081 atgacgaaac ggcctgacga cacggccgag ccgatggccg tatttgccga gttcctcgag
     1141 acgaccagcc gcgaggtgga cgacgtgatg gagcggctga tgccgcccgt cgcctcgatg
     1201 ccgaaggtga tccaccgggc catgcgttac agcctctttg cgggtggcaa gcggatccgg
     1261 ccggccctgg tggtgctcgg ttatcgcgcc tcggggggcg ggggcaaggt ggcgcacacg
     1321 gccggcgcgg ccatcgagct catccacacc ttctcgctca tccacgacga cctgccgtgc
     1381 atggatgacg atgactatcg ccgcggaaag ctttcgaacc acaaggtctt cggcgaaggc
     1441 gtggccgtcc tggcgggcga cgcgctccat gcgctcggct tcgatgtgct ggcggggctg
     1501 cgcaagttca agggcgtgag cgccgagacg gtgctcgacg tgatggacga ggtggggcgc
     1561 gccatcggca ccgacgggat gatcggcgga caggtgatgg atctcgagtg ggagggggcc
     1621 aaaccctcgc ggtcgaaggt cgactacatc caccgccgga agacggccgc actcctcggc
     1681 tcctcgctcg tggtgggggg gatcctggcg ggcgccgaca agcccaccat caaggccctc
     1741 cgtgcctatg gcgaaccgtt cgggctggcg ttccagatcg tcgacgacat cctgaatgtg
     1801 caacaggccg gccgggcgct ggggaagaag cccggcggcg acgagcgcct cggcaagatg
     1861 acctggccgg cggtgcacgg cctcccgagg agccaggccg acgtgacgcg cctcctcgac
     1921 gaggcctcgc gcgcggcccc gaaattcggc ccctggatgg accacttcct cggcctggcc
     1981 gactacatct cggtacggcg gcaggcgcgc cggtgaggga gttcatcgcg gcgcgcccct
     2041 ggatcatcgc cctcatggcc ggctcgctgg tgcagctcat gaagttcgcg gcggactggg
     2101 ggcgccacaa gcggcccaat ttccgggtgc tcacctctac cggtggcatg ccctcgagcc
     2161 actcggcggg tgtctgcgcc ctgacgaccg ccatctacgt cacgacgggg gggcgttcgg
     2221 ccctcttctc cgtggcgctt tatttcagcc tgatcgtcat gtacgatgcc actggcctcc
     2281 gccgttcggc ggggctgcaa gctgctatcc tgaaccgaat tgtggacgaa cagttcatcc
     2341 atcaccggaa ggccggggag gggcgcctca aggagctcct gggacacacg cccctcgaag
     2401 tgctcgtcgg ggccatcctc ggcatcctgt tcgccctcac ctggtacccc tcgggcggct
     2461 gacccccacg aaaaaggacg gtatgggacg ctatctcgac atggtcgact ccccgcgcga
     2521 cctcaagaaa ctctcgccgg aacagctcaa gacgctggcg gacgaagtcc gcgaggagat
     2581 catccacgtc atcagccaga cgggggggca cctcggcgcc tcgctcggaa ccgtcgaact
     2641 gacggttgcg ctgctgcatg tcttcgattc gcccatcgac aagatcgtct gggacgtggg
     2701 gcaccaggcc tatccgcaca aactcctgac cgggcgcaag caacgcttcc cgaccatccg
     2761 ccagtacggc gggctttccg ggttcctccg ccgcgacgag tccgagtacg acgccttcgg
     2821 cgccggccac gcctccaccg ccatctcggc cgccctcggc atggccgccg cgcgtgacca
     2881 gcaggggggc acgaacaagg tggtggcggt catcggcgac ggcggcctca ccggcggcat
     2941 ggccttcgag gccctgaaca acgcgggcga cctgaagagc gacatcatcg tggtggtcaa
     3001 cgacaacgag atgtcgatca gcccgaacgt gggcgccatg accaagtacc tcacgcgcct
     3061 caccgccgcg cccatctacc gccgcttcga gaccgacgtg tacgacctgc tcggcaagat
     3121 cccggcggtg gggcacccgg cgcaggaact ggccggccgc gtgaaggaaa gcctgaccaa
     3181 tctcgtggtg ccgacggtct tcttcgagga gctcggcttc aagtacttcg gcccgatcga
     3241 cggccacaac ctcgacggcc tggtcgacac gatgaagcac gtcaaggaaa tcggcgggcc
     3301 gatcgtgctc catgtgctca cacgcaaggg gaaggggtac aagccggccg aggccgaccg
     3361 cctcgcgctc cacggcgtca cccagttcga caagacgacc ggcgccatga agaagaagga
     3421 agccaaggcg cccacctaca gccagatctt cggggaggcg ctcaccgaga tcgccaaccg
     3481 cgatcccaag gtggtggcca tttcggccgc catgatcgag gggaccggcc tccgcatctt
     3541 cgagaagtcg cacccgacga agatctacga cgtcggcatc gccgagcagc acgcggtcac
     3601 cttcgccgcc ggcatggcca ccgaggggat gaagccggtc tgcgccatct actccacttt
     3661 cctgcaaagg gcctacgacc agatcgtgca tgacgtctgc ctgcagaacc tcgatgtcac
     3721 gttcgtgctc gatcgcgccg gactggccgg ggatgatggc gccacccacc acggcgtctt
     3781 cgacatggcg tggctccgca ccgtccccga gatggtgatc atggcgccga aggacgagtg
     3841 tgagatgcgc gacatgctct acacggccat cgagcatccc ggcccggcgg cgctgcgcat
     3901 cccgcgcggc gagggttcgg gcgcccggat gaacaaggac ttcacgaagc ttccgatcgg
     3961 cgagggagaa atcctctccg acggcgcgga cgtgacgatc atcggcatcg gcgccatggt
     4021 cgagcaggcc gtgcgcgtgg ccgaggagct gggcgcgcag ggaatcaggg ccggcgtggt
     4081 gaacgcccgg ttcgtgaagc cgctcgacac gcagctcatc ctcgaaaggg cccgtaagac
     4141 cgggcggctc gtggtgatcg aggagcactc gccgcacggc ggtttcggtg cggcggtcct
     4201 cgagtgcctg tgcgacgccg ggctgtcgaa cattccagtg ctgcacgcgg cgctccccga
     4261 tcggttcgtg caccatgggg
//
LOCUS       DASVOD010000241         4818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_486510, whole genome shotgun sequence.
ACCESSION   DASVOD010000241 DASVOD010000000
VERSION     DASVOD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4818
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486510"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1337)
                     /gene="tssM"
                     /locus_tag="VF720_05090"
     CDS             complement(<1..1337)
                     /gene="tssM"
                     /locus_tag="VF720_05090"
                     /inference="COORDINATES: protein motif:HMM:NF025689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HEX7878760.1"
                     /translation="MILAIIKRTLLPFGLPVVLITAVFFLARPRVPFLKNLPSWLFWA
                     LLLAILLVWVIVIVWSWLAARRRARAIEQGLLKQAQWGVENAAPSRRGDLEEIRKSLL
                     EAMAMLKNSPQGKRALYDLPWYLVIGPPGIGKTTAIVNSGLNFPGLTAARRMRGTGGT
                     RQWDFWFSTDAILLDTAGRYALSEDRTEAEGEWFGCLELLRKHRPKGPINGLILGYSA
                     EALGQQDEAQIVHDARELRQRLDEMMDKLKWSFPVYVVITKCDLIAGFADFFSGLSPS
                     ERQQVWGTAYAVEDASGRPVGDRFRAGFASLVRRLKEFRIRRMGRSEKGESWGKAFLF
                     PEEFAALENRAHLFIETLFEANPFRRDVPIFRGLYFSSGRQMGRPFDTVISQIQSMLG
                     GSKTMAESQPEKEDAFFVRDLFAKVLKGDRDLVGHTDSARKKNLRFQIAASTGL"
     gene            complement(1339..2070)
                     /gene="icmH"
                     /locus_tag="VF720_05095"
     CDS             complement(1339..2070)
                     /gene="icmH"
                     /locus_tag="VF720_05095"
                     /inference="COORDINATES: protein motif:HMM:NF021377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HEX7878761.1"
                     /translation="MTALLKRAPKGSAPGSAVASAPPAAVDLTPRLADHAGSLLSLIL
                     LLRRSSDLDQFKDLRLTVERLIAEFRARSRDDGASGTDVDDATYALAATIDEILLNAR
                     WAGRDAWERDTLARTWCQDEFVGIGFYDKLNQIRRSGTARPQALEVFYYCLVAGFQGK
                     LVEDPAQWNKLVEDLAPDIAPPVKILSPEGQLAEREGAGNRLASFPWPAVLITAIFLP
                     LLVLLLAYGIVDRNRDAILKALAGH"
     gene            complement(2067..3434)
                     /gene="tssK"
                     /locus_tag="VF720_05100"
     CDS             complement(2067..3434)
                     /gene="tssK"
                     /locus_tag="VF720_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX7878762.1"
                     /translation="MSRVHRVLWSEGQFLLPQHFQQWDLFQESQTSGARLLTQAWPWG
                     VAEMEIDNEMLARGTFRVMRLSALLPSGARIEAPDVDPLPPSIPFKDAFDSRADSMDI
                     FVGLPMRRNGWANCLLPGESGDAAGEVRFVARPVNVPDENSGRNDRSILRADQNLRLL
                     LGSDQRDDFETIPLARVVRSSAGGYQLHAEFVPPLLSIRASSVVSEMVRDLVERLSAR
                     STELAAKFTESGADARDVTNANLRAFMIFFTVNGTLPLLAHARNAPNTHPEMLYRLLT
                     GLAGQLSTFNSRRLHPRDLPDYDHLEPGPIFQSLRSTLHQLMELEIGVGYEVIPLIPA
                     GEGRLTGRIPRASFFEPGAALILTVGGDSIGESEVQGGMRRLVVASVDRIQQKINNRL
                     PGLTLHPLAVPPPSIPRRRNTWYFQLDSRGADFDAIRAAMNLALDVPSELQPATLELL
                     GLESK"
     gene            complement(3439..3912)
                     /locus_tag="VF720_05105"
     CDS             complement(3439..3912)
                     /locus_tag="VF720_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878763.1"
                     /translation="MNKVLVARTRASHFLSLATALMAFSLSGCLGCGGDSFIRADLER
                     GAGLKQPAWVGVYYLSQESAIDAVDAADLVSRTKAEEKLAGVQGIVMKEINPVYPDEP
                     VSILKEAPDPAIRWVVIVANLKGGACARQKVALKKGETVKFHVTVEEQCLVVKKD"
     gene            complement(3909..>4818)
                     /locus_tag="VF720_05110"
     CDS             complement(3909..>4818)
                     /locus_tag="VF720_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878764.1"
                     /translation="MGSETRRNRVFRGARISPAETARRLRLTLASPEFRSENQRKAAL
                     RRILHEAVAGLDSPSARVYVDELRGRFPDRVYEATNRLQESESARKELEKKVETMAGE
                     RDRMTMRLRAAEALLTGLLEPPAGAKSAAGAGGGGEALLGPLLDAEGIRRLAPVLARL
                     MAFVAAQETAMAGVDETVGRSGGAGSGVHDLVVKLARGEGSPETSVAELERRLANLTR
                     LPAALLAGLMQSWKAGTQGVLEELDPRKVEAEIPKKLPGLREAAVLKEQRRRYEQFWD
                     DLDKNVEHYYRDVFRRIYVEKMEGRS"
BASE COUNT          779 a         1572 c         1704 g          763 t
ORIGIN      
        1 agtccggtgg aggcggcgat ctggaaacga aggttcttct tccgcgccga gtcggtgtgc
       61 cccaccaggt cgcgatcccc cttgagcacc ttggcaaaca ggtcgcgcac gaagaaggcg
      121 tcttccttct cgggttgcga ttcggccatc gttttcgagc cgcccagcat cgactggatc
      181 tggctgatga ccgtgtcgaa cgggcgcccc atctggcggc cgctggagaa gtagaggccg
      241 cggaagatcg gcacgtcgcg ccggaaggga ttggcctcga agagcgtttc gatgaagaga
      301 tgcgcgcggt tctcgagcgc ggcgaactcc tcggggaaga ggaacgcctt cccccagctt
      361 tcccccttct cggagcggcc catccggcgg atgcggaact ccttgagacg tcggaccagg
      421 ctcgcgaaac cggcacggaa gcgatcgccc accggacggc cggaggcatc ctccacggcg
      481 taggcggtgc cccacacctg ctggcgctcc gacggcgaga gtcccgagaa gaagtcggcg
      541 aagccggcga tgagatcgca cttggtgatg accacgtaca ccgggaacga ccacttgagc
      601 ttgtccatca tctcgtcgag gcgctggcgc agctcgcggg cgtcgtggac gatctgcgcc
      661 tcgtcctgct ggccgagggc ctcggcgctg tagccgagga tgaggccgtt gatgggcccc
      721 ttcggccggt gcttgcgcag gagctcgagg cagccgaacc attctccctc ggcctcggtg
      781 cggtcttcag agagcgcgta gcggccggcc gtgtcgagca ggatggcgtc ggtcgagaac
      841 cagaaatccc actggcgcgt gcccccggtg ccgcgcatgc ggcgggccgc ggtgaggccg
      901 ggaaaattga ggcccgagtt gacgatggcg gtggtcttgc cgatgccggg agggccgatc
      961 accaggtacc aggggaggtc gtagagggcc cgcttcccct gcggcgaatt cttgagcatg
     1021 gccatcgctt cgaggaggct cttccggatt tcctcgaggt cgccgcggcg cgacggcgcg
     1081 gcattctcca cgccccactg cgcctgcttg agcagcccct gctcgatggc cctggcgcgg
     1141 cggcgcgccg ccagccagct ccagacgatc acgatcaccc agacgaggag gatggccagg
     1201 aggagcgccc agaagagcca cgacggcagg ttcttgagga acggcacgcg gggccgcgcc
     1261 aggaagaaga cggccgtgat caggacgacg gggaggccga atggcaggag cgtgcgcttg
     1321 atgatggcga ggatcatgtc agtgacccgc cagcgccttg aggatggcat cgcggttgcg
     1381 atccacgatg ccgtaggcca acaggagcac gaggagcggc aggaagatgg cggtgatcag
     1441 caccgccggc cacggaaacg aggccaggcg attgccggcc ccttcccgct cggcgagctg
     1501 cccctcgggc gagaggatct tgaccggggg cgcgatgtcg ggcgccaggt cttcgaccag
     1561 cttgttccac tgggcgggat cttccaccag ctttccctgg aaaccggcca cgaggcagta
     1621 gtagaacact tcgagcgcct gcgggcgggc cgtgccgctc cggcggatct ggttgagctt
     1681 gtcgtagaaa ccgatgccga cgaactcgtc ctggcaccag gtgcgggcca gcgtgtcgcg
     1741 ctcccaggca tcgcgcccag cccagcgggc gttgaggagg atttcgtcga tggtggcggc
     1801 aagggcgtag gtggcgtcgt ccacgtccgt gcccgaagcg ccgtcgtcac gcgagcgggc
     1861 ccggaattcg gcgatgagac gctccaccgt gaggcggagg tccttgaact ggtcgaggtc
     1921 gctcgagcgc cggaggagga gaatcaggga aagaagcgag ccggcgtgat cggccaggcg
     1981 cggcgtgagg tcgacggcgg cgggcggcgc agaggcgacg gcgctgccgg gcgcgctgcc
     2041 tttcggagct cgcttcagca gcgccgtcac ttcgactcca gcccaagcag ctcgagcgtg
     2101 gccggctgga gctcggacgg cacgtcgagc gccaggttca tggccgcgcg gatggcgtcg
     2161 aaatcggcgc cgcgcgaatc gagctggaag taccaggtgt tccggcggcg cgggatggag
     2221 ggaggcggca ccgccagtgg atggagggtg agccccggca accggttgtt gattttctgc
     2281 tggatgcggt cgaccgaagc caccacgagg cgcctcatgc cgccctgcac ttccgattcg
     2341 ccgatcgaat cgcctcccac ggtgaggatg agcgcggcgc ccggctcgaa gaagctggcc
     2401 cgcgggatgc gtcccgtgag gcgcccctcc cctgccggaa tcagcgggat cacctcgtaa
     2461 cccacgccga tctcgagctc catcagctgg tgaagcgtgc tgcggagcga ctggaaaatc
     2521 ggtccgggct cgaggtgatc gtaatccgga aggtcgcgcg ggtgcaggcg tcgggaattg
     2581 aacgtggaga gctgcccggc caggccggtc aacagccgat agagcatctc gggatgcgtg
     2641 ttcggggcat tcctggcatg ggccaggagc ggcagcgtgc cgttcaccgt gaagaagatc
     2701 atgaaggccc gcaggttggc attggtcacg tcgcgcgcat cggcgcccga ttcggtgaac
     2761 ttggcggcga gctccgtcga acgcgcggag aggcgctcga ccaggtcgcg caccatctcg
     2821 ctgacgaccg aggaagcccg gatggaaagg agcggcggca cgaactcggc gtggagctgg
     2881 tagccgccgg ccgagctgcg cacgacgcgg gccaggggga tcgtctcgaa gtcgtcgcgc
     2941 tggtcgctgc cgagcaggag gcggagattc tggtcggccc ggaggatcga gcgatcgttg
     3001 cgccccgagt tctcgtcggg cacgttgacg gggcgggcca cgaatcgcac ctcgccggcc
     3061 gcatcgcccg attcacccgg caggaggcag ttggcccagc cgttccggcg catcggcaat
     3121 cccacgaaga tgtccatcga atcggcgcgg ctgtcgaagg cgtccttgaa ggggatcgac
     3181 ggcggcagcg gatcgacatc cggcgcctcg atgcgcgcgc ccgagggaag aagcgccgag
     3241 aggcgcatca cccggaacgt gccgcgggcc agcatctcgt tgtcgatctc catctcggcc
     3301 acgccccacg gccaggcctg cgtgaggagg cgggcgcccg acgtctgcga ttcctggaag
     3361 aggtcccact gctggaagtg ctggggaagg aggaactgcc cctcgctcca gaggacgcgg
     3421 tggacgcggc tcatcgtgtc agtccttctt gacgacgagg cactgttcct cgaccgtcac
     3481 gtggaatttc accgtctcgc ccttcttgag cgccactttc tggcgggcgc aggcgccccc
     3541 cttcaggttg gccacgatca ccacccagcg gatggcggga tcgggcgcct ctttcaggat
     3601 ggaaaccggc tcgtccggat agacggggtt gatctccttc atgacgattc cctggacgcc
     3661 cgccagcttc tcctcggcct tcgtgcggct gacgaggtcg gcggcgtcca ccgcgtcgat
     3721 ggccgattcc tgcgagaggt aatacacccc tacccaggcc ggctgcttga gccccgcgcc
     3781 ccgttccagg tcggcgcgga tgaacgaatc gccgccgcag cccaggcatc ccgacaggga
     3841 aaacgccatg agcgcggtgg ccaacgacag gaaatgcgat gcgcgtgtcc gcgccaccag
     3901 caccttgttc acgaccggcc ctccatcttc tccacgtaga tgcggcggaa cacgtcgcga
     3961 tagtagtgtt ccacgttctt gtcgagatcg tcccagaact gttcgtaacg ccgccgctgc
     4021 tccttgagca ccgcggcttc gcgcaggccc ggcagcttct tcggaatttc ggcttcgact
     4081 tttcgcggat cgagttcctc gagcaccccc tgcgtgccgg ccttccacga ttgcatgagg
     4141 ccggccagca acgcggccgg caaacgggtg agattggcca gccgccgctc gagctcggcc
     4201 accgaggttt cggggcttcc ttcgccgcgg gccagtttca cgaccaggtc gtgcacgccg
     4261 gacccggcgc cgccgctgcg cccgaccgtt tcatccacgc cggccatggc ggtttcctgc
     4321 gctgccacga acgccatgag gcgcgcgagc accggggcca ggcgacggat cccttcggca
     4381 tcgagcagcg gaccgagcag ggcctctcca ccgccacccg cgcccgcggc cgatttcgcg
     4441 ccggccggcg gctcgaggag tccggtgagg agcgcttcgg ccgcccggag gcgcatggtc
     4501 atcctgtcgc gctcgccggc catcgtctcg actttttttt caagctcctt gcgggccgac
     4561 tccgactcct ggagccggtt ggtggcctcg tagacgcggt ccggaaagcg gccgcggagt
     4621 tcgtccacgt agacgcgggc cgagggggaa tcgagcccgg ccacggcttc gtggaggatc
     4681 cgccgcaacg ccgctttccg ctggttctcc gagcggaact cgggcgaggc cagtgtgagg
     4741 cggaggcgcc tggccgtctc ggccgggctg atcctggcgc cgcggaagac gcggttccgg
     4801 cgggtctcgc ttcccatg
//
LOCUS       DASVOD010000242         4846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_486556, whole genome shotgun sequence.
ACCESSION   DASVOD010000242 DASVOD010000000
VERSION     DASVOD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486556"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2526
                     /locus_tag="VF720_05115"
     CDS             <1..2526
                     /locus_tag="VF720_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX7878765.1"
                     /translation="AWPWRVREHRRQSRALTRLLGLSAGERAEIRAADALTPVIARRL
                     DDEEDYIGALVVAAEQLDRRRRVLGNDDPEVAATLDALGRIAYASGEVLRAEAYLEQA
                     VAIRRTLLGGDDPRVAQSLRSLGVVMEERRQKETARAFFLEATAIAEKGWSPGDSALA
                     PIYESLGSHYRRWAEWKQAEAFYERALAIRRACFGPRSVPVAENALWLGHCQLCAGKL
                     DEAERSLTEAETLLAENGLGNSHPMGDATLFLATIHWLRGDNAGAIARWRHSYACPTD
                     VSRKLPQSYGLRRLGGIRGEWIDAQLAEGHSDEAWREVCRGSGPVTRALLEFARIRRE
                     NPETSARLDSLRAAVIEVRRRLIDIADPDNAPPSVMVPLLSELASIEARRLETERLLT
                     SHIPLPDGDVPRLADVQARLGDDQAMIGWIFGKWSWPGRTQKWAFHAYVVRRDGPVHW
                     VDYGSFTDPDASGARFRSLQSVEAILDKASRWPHRVAYDVTLDSLGADAGRVVFGPLE
                     PYLEGVSEIIVAGRSSLMPGMGGTSECWRGSDGVVLGERFAFSYTPSPWAFVALADAG
                     KERDLSLVAVADPLGPDREPPDRLPWPGSAFPDEATASAAVIDASLLRSAIGGDPAAM
                     ARLPELPWSRTEVMTVANAFPRSTLLIGPQATEASLERIVRDRSCGVLHFATHALIDD
                     AEPDRSTLVMAGATNALADDDPDDDGRITAEEVLLGWNLDADLVTLSGCRTSEGQPAR
                     GGENLGFAQAFLASGANCLLMSRWPVDDRATALLMGRFYENLSGTYTDERRGRIHEPM
                     SRARALQEAQLWLRDWRDPKGRATFAHLVCWAGFVLIGTANKP"
     gene            2633..2857
                     /locus_tag="VF720_05120"
     CDS             2633..2857
                     /locus_tag="VF720_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878766.1"
                     /translation="MSELIERGLHYIYDSRAFRNLKDQDRIVAVNGFGEQAVGTYDET
                     SCSIGGLRGKVTYWRHAVPADERLATTGYN"
     gene            complement(2879..4117)
                     /locus_tag="VF720_05125"
     CDS             complement(2879..4117)
                     /locus_tag="VF720_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878767.1"
                     /translation="MMPRRLRRPTSRGKFPGRCPGAFPGLFLGVLLVALLVALTAARA
                     AMALDLYWIESSYGVPKLNRSDLAGTGVTTVMLTPGTLPQGLAVRNNGRPVWAEGAWT
                     NARIMESGYNLSGVVPIVTGQSTLNGVAVDPLNQVTFFTSSNLSLGARIHRKTLNGAA
                     TTLMTLPAAANPRGIAVDPAANRIFWADFDQNTINRANLDGTGITTIVNLGSDARPWG
                     ISVNATLQRVYWTEYGGGRVMRSGYGGEAVTSILTGLTNPTYLASDATAGFHFWTEGG
                     ANTERIRRANLDGTGLLTLPATISTYGGIAVGPGAVTGVPAEPGAVLLTLAPPWPLPA
                     AGAVHLSFTLPSAGFVRLAIFDVLGREVAVPVNESMAAGPHVAIWDGHVDGSPAAVGV
                     YFARMVMNGEARTERVVVAR"
     gene            complement(4114..4752)
                     /locus_tag="VF720_05130"
     CDS             complement(4114..4752)
                     /locus_tag="VF720_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEX7878768.1"
                     /translation="MTRERPGEPLERRTFLGRVLATAAGLILPMRPGRAGAATQDADP
                     YLGQIMPVAFNYAPRGWALCNGQLLPINQNQALFSILLTTYGGNGITTFALPDLRGRS
                     AINFGQGPGLTNRPIGQAIGSDLHQLTIAEMPSHTHVPRAASATATSVIPSGAVVSAR
                     NPAQVPEWGTGLEAAMHAGAIQNSGNGQSHANQQPYLTVNYIIAIQGIYPQP"
BASE COUNT          720 a         1635 c         1736 g          755 t
ORIGIN      
        1 gcctggccgt ggcgagtccg cgagcaccgt cgccagtcca gggcgctcac ccgcctcctc
       61 ggcctttccg ccggggaacg cgccgaaatc cgcgcggccg acgccctgac cccggtgatc
      121 gcccggcggc tggacgatga ggaggactac atcggcgccc tggtggtggc ggcggagcag
      181 ctcgatcggc gccggcgggt cctggggaat gacgatcccg aggtggccgc cacgctggac
      241 gcgctcggcc gcatcgccta cgcgtcggga gaagtgttgc gggccgaggc ctatctcgag
      301 caggccgtgg ccatccgccg caccttgctc gggggcgacg acccgcgcgt ggcgcaatcg
      361 ctgcgcagcc tcggtgtggt gatggaggag cgtcgccaga aggaaacggc gagagccttc
      421 ttcctcgagg cgacggccat cgcggaaaaa ggctggtcgc cgggcgattc cgcattggcc
      481 ccgatctacg aatcgttggg gagccactac cggcgctggg ccgagtggaa gcaggccgaa
      541 gccttctacg agcgggccct cgcgatccgg cgcgcctgct tcgggccccg cagcgtgccc
      601 gtggcggaga atgcgctctg gctggggcac tgccagctct gcgccggcaa gctcgacgaa
      661 gccgaacgat cgctgacgga ggccgagacg ctcctggccg agaacggcct cgggaacagt
      721 catcccatgg gggacgccac gctcttcctg gccaccatcc actggctgcg gggcgacaat
      781 gccggcgcca tcgcccggtg gcggcattct tatgcctgcc ccacggacgt gtcgcgcaag
      841 ctcccgcaga gctacggact ccggcgcctc ggcgggatcc gcggcgagtg gatcgacgct
      901 cagctggccg agggacactc cgacgaagcc tggcgtgaag tgtgccgcgg ctcggggccg
      961 gtgacgcggg cgctcctcga attcgcccgc atccgtcgcg agaacccgga aaccagcgca
     1021 cggctcgact cgctgcgtgc cgcggtgatc gaggtccggc ggcgcctgat cgacatcgcc
     1081 gacccggaca acgcgccccc ctccgtgatg gtgccgctcc tttccgagct ggcctcgatc
     1141 gaggctcgtc ggctggagac cgaacgcctc ctgacgagcc acattcccct gccggacggg
     1201 gatgtcccgc gtctggccga cgtccaggcc cgcctgggcg atgaccaggc catgatcggt
     1261 tggatcttcg gcaagtggag ctggccgggg cgcacgcaga agtgggcgtt ccatgcgtac
     1321 gtcgttcggc gcgacggccc ggtgcactgg gtggactacg gctcctttac cgatcccgac
     1381 gccagcgggg cgcgattccg gagcctccag tcggtcgagg ccatcctcga caaggccagc
     1441 cggtggccgc atcgcgtggc gtacgatgtc acgctcgatt cccttggcgc ggacgccggc
     1501 cgggtcgtct tcggtcctct cgaaccgtac ctcgagggcg tctcggagat catcgtggcc
     1561 gggcgatcga gcctgatgcc ggggatgggt ggcacctcgg agtgctggcg cgggagcgac
     1621 ggcgtggtgc tcggcgagcg ctttgccttc tcgtacacgc catctccctg ggctttcgtt
     1681 gcgctggccg atgccggaaa ggagcgcgat ctctccctcg tggccgtggc cgatccgctc
     1741 ggcccggacc gggagccgcc cgaccggctg ccctggcccg gctcggcctt tccggacgaa
     1801 gccaccgcct ccgcggccgt catcgacgcc tccctgctcc ggagcgccat cggcggcgac
     1861 ccggccgcga tggcccggct ccccgagctc ccctggtcac gcaccgaggt catgacggtc
     1921 gcgaacgcct ttccgcgcag cacgctcctc atcgggccgc aagccaccga agcctcgctg
     1981 gaacggatcg tgcgggaccg gtcgtgcgga gtgctccact ttgccaccca cgcgctcatc
     2041 gacgacgctg aacccgatcg atcgaccctc gtgatggccg gtgccacgaa cgctctggcc
     2101 gatgacgacc ccgacgacga cggacgcatc accgcggagg aggttctcct cggctggaac
     2161 ctcgatgccg acctggtgac gctgagcggc tgccgcacgt ccgagggaca gcccgcgcgc
     2221 ggcggcgaga acctcggatt cgcgcaggcc ttcctggcca gcggcgccaa ctgcctcctg
     2281 atgagccgat ggccggtgga tgatcgggcc acggcgctcc tgatgggccg cttttacgag
     2341 aacctgagcg gcacgtacac ggacgagcgc cgcggccgca ttcacgagcc aatgtcgcgc
     2401 gcgcgcgcgc tgcaggaggc ccagctctgg ctccgggatt ggcgggaccc gaaagggcgc
     2461 gccacgttcg ctcacctggt ctgctgggcc gggttcgtgt tgatcggcac cgccaacaag
     2521 ccctgatgct cgaatcggga gcctcccgat tccgcatcca ccttgaactc ccgcatcctt
     2581 ccgcgactgg ccattgtgtc accctctctt gaaagcgggg ctggtcgtga acatgagcga
     2641 gttgatcgag cgcggacttc actacattta cgattcccgc gccttccgga acctcaagga
     2701 ccaggatcgc atcgtggcgg tgaacgggtt cggcgagcag gccgtcggca cctacgacga
     2761 gacgtcgtgc tccatcggcg ggttgcgcgg aaaggtgacg tactggcgtc atgcggtgcc
     2821 ggcggacgaa cgccttgcaa ccaccggcta caactgacgg ctgaatttcc ggccgccgtt
     2881 acctcgccac cacgacgcgc tcggtgcgcg cctcgccgtt catgaccatg cgggcgaagt
     2941 agacgcccac cgcggcgggc gaaccatcga catgaccgtc ccagatggcc acgtgcgggc
     3001 cggcggccat cgactcattc acgggaacgg ccacctcacg ccccagcacg tcgaagattg
     3061 ccaggcgcac gaagcccgcc gacggaagcg tgaatgacag gtgcacggcc cctgccgccg
     3121 ggagcggcca tggaggtgcg agtgtcagca acacggcacc gggctccgcc ggcacgccgg
     3181 tgaccgctcc gggcccgacc gcgatccccc catacgtgct gatcgttgcg ggaagggtca
     3241 ggaggccggt gccatccagg ttggcgcggc ggatccgctc ggtgttggcg cccccctccg
     3301 tccagaagtg gaaaccggcc gtggcatcgc tggccaggta ggtgggattg gtgaggccgg
     3361 tgaggatgct ggtaacggcc tcgccgccgt agcccgagcg catgacgcgg ccgcctccgt
     3421 actcggtcca gtagacgcgc tgcagggtgg cattgacgct gattccccac ggccgggcat
     3481 cggacccgag gttgacgatc gtggtgatgc ccgtgccgtc caggttcgcg cggttgatcg
     3541 tgttctggtc gaagtcggcc cagaagatcc ggttggcggc gggatccacg gcgataccgc
     3601 gcggattggc ggcggccggc agcgtcatga gcgtggtggc ggcgccgttc aacgtcttcc
     3661 ggtggatgcg cgcaccgagc gaaaggttgc tcgaggtgaa gaatgtcacc tggttcaacg
     3721 ggtcgaccgc cacaccgttg agcgtggact ggccggtgac gatcgggacg acgcccgaca
     3781 ggttgtaccc ggattccatg atacgtgcgt tcgtccaggc gccctcggcc cagacggggc
     3841 gcccgttgtt tcggacggcc aggccctgcg gcagcgtgcc gggggtgagc atcacggtcg
     3901 tcacgccggt gccggccagg tcggatcgat tgagcttggg aacgccgtaa ctgctttcga
     3961 tccagtagag atcgagcgcc atcgccgcgc gggccgccgt gagcgcgacg aggagcgcga
     4021 cgaggagcac gccgaggaac aggccgggga atgcgccggg gcacctgccg gggaacttgc
     4081 cgcggctcgt gggccggcgg agacgccgtg gcatcatggc tgcgggtaga ttccctggat
     4141 ggcgatgatg tagttgacgg tgaggtacgg ctgctggttc gcgtgggact ggccgttgcc
     4201 gctgttctgg atggcccccg catgcatggc cgcctccaga ccggtgcccc actccggcac
     4261 ctgtgccggg ttgcgcgccg acaccaccgc gccggaggga atgacgctgg tggccgtggc
     4321 ggaagcggcg cgcggcacat gcgtgtgcga cggcatctcg gcgatcgtga gctggtggag
     4381 gtcgctcccg atggcctggc cgatcggccg gttggtgagg ccgggcccct gcccgaaatt
     4441 gatcgccgag cggccgcgga ggtccgggag tgcgaacgtg gtgatgccgt tgcccccgta
     4501 cgtggtcagg agaatcgaga aaagggcctg gttctggttg atgggcagga gctgcccgtt
     4561 gcagagggcc cagccgcgcg gcgcgtagtt gaacgccacc ggcatgatct ggccgaggta
     4621 ggggtcggca tcctgcgtgg ccgcccccgc ccggccgggg cgcatcggca ggatgaggcc
     4681 tgctgccgtg gccaggacgc ggccgaggaa ggtgcgtcgt tcgagcggtt cgcccgggcg
     4741 ttccctcgtc atgacggcac cggccacaaa ccgtcgacgg cgatgatgta ccggatgccg
     4801 atccagggct gcatgttggg atggggcgcc gagcttccgg cggtgg
//
LOCUS       DASVOD010000243         9463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1009185, whole genome shotgun sequence.
ACCESSION   DASVOD010000243 DASVOD010000000
VERSION     DASVOD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9463
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009185"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VF720_05135"
     CDS             <1..198
                     /locus_tag="VF720_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878769.1"
                     /translation="EIFDAAGRLVRVLVAEEQSAGRHEPMWQGTDESGRDVLSGIYFA
                     RLTTSHNGTQTASQRKINLVR"
     gene            complement(211..2904)
                     /locus_tag="VF720_05140"
     CDS             complement(211..2904)
                     /locus_tag="VF720_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX7878770.1"
                     /translation="MDRPRRLIPVLILAALTPLIAAPAFATNYTWKSAINGSWHTSSN
                     WLPTGVPGPSDVAIINLSGNYTVSVNSNASVGAVGLSGGTGSRRLTVNSATLTTGTGI
                     AVGTAHVLNLNSGTISGAGTLDLQGSLTSSGTSHLNSPVMTHAATAITISGTSLEVAG
                     GFELHGNLETNPLSSGRLIVGSGVLTNSGTLDIENGDVTGNVTNGAGNTISLLNSTMQ
                     GSVVNLGTLSIQGTSSLLAFLDNEGLVTIQNGVLLASGDTGHQNYGTIRVQGGTFTVT
                     QTPAGYFSNQGTLEVFPGAGVAITGGAFRNQPTGILSGGGTCDMSGTSFLNDGTIKPG
                     ASPGTLTVNGDVILGSTSVVEMEIGGTVPGSSQDRLAISGIVTLAGTLNVSLVSGFMP
                     QPGEAFELITCGGHTGQFTTVNGTDLGGGRYLAFVPQTRVSDPIRLTALSETWTNVAA
                     TGPAARLAHTAVYDGFSNRMIVFGGLGPAGELNDTWVLTNANGSGGTPAWQPLSPGNA
                     PPLARQGHAAAYDGVNNRLIVYGGYSAARPEGFLDVWVLSNANGLGGTPMWTQLLPGG
                     STPDRRAGSGIAYNPSSNRLILFGGLRTLDPCAGASNDVWILTHANGLGGTPEWSLLP
                     ATGPLPAARSGAVLGYDSATNRLIVQGGTVACTWTIGEAHYLTHADGLGGTPTWSTLP
                     LVNPAPPGRTYHGGIYDAATDRLLTFGGIDANGTMALDDILLVTGATNAGAAGWSRLP
                     NPTVHPPTRSSHTACWDPLTRRLIVFGGDGWQYGLLGDLWILDLDLDNGAVSGLPDPA
                     PARPGTNAPGFGFRAVWPNPTVGPAALRFALPRAASVSVDVFDVSGRHVRQLLNGELA
                     EGVHDIQWNGDGYAGPVPSGIYYVVIRSGGGRDARRLVVTR"
     gene            3067..3210
                     /locus_tag="VF720_05145"
     CDS             3067..3210
                     /locus_tag="VF720_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878771.1"
                     /translation="MAPNRRAPGTRFNVDNAVYVDTARAGRTCPQASGAMAMEGSDPN
                     HDT"
     gene            3248..4687
                     /locus_tag="VF720_05150"
     CDS             3248..4687
                     /locus_tag="VF720_05150"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7878772.1"
                     /translation="MTLLFLLVISLATAGAACGEPPSAGASPRVGPTDLAEFSAFIDS
                     IMTAEMAAAHIPGAAFVFIRDHRVILARGYGLADVEAHRAVAVDSTIWRVGSISKVFT
                     ATAVMQLVDRGLVELDAPIDRYVHKVSIPATFPDPVTVRQLLDHTAGFDEIRPGTQAA
                     SRDSLLPLADFLRHRLVRVRPPGRTIAYSTYGMTLAGELVEEVSGQPFERYMEENIWR
                     PLGMSRTCINVPERWLGHTARGYELSGDSLAVQPWEWYHTIPASSINATASDMARFLL
                     AHLDYGAAASERLFDERTGMEMQRQQVTMDPRLPGFALGFYEDYVGKLRVLEHGGNTA
                     GFSAELLLIPGHHAGFFVVNHLEGSRLRDNLKWALLQRYYPAARERHQVPPLTTAAPS
                     SAVRFAGQYVPTSSCWSCRPVRASSLFDVTSNPDGSLTMAGGRWIPVDSLLFVNEKGT
                     GYIAFRANSSGAITHCFPGGFWSFQRVPE"
     gene            complement(4635..5756)
                     /locus_tag="VF720_05155"
     CDS             complement(4635..5756)
                     /locus_tag="VF720_05155"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7878773.1"
                     /translation="MKLWLACLVVLTMGGCSSADPDVPPPARTLAEFEVQLDDLRAEL
                     AIPGMSAIVMRHDTVVWEGALGVADIASGLPVTSTTVFHLASLTKTFASTIVLQLVDQ
                     RLVDLDDPITDYGLSLPGQGIIRVRHLLSHTSEGIPGENFHYNGDRYALLDQVIGRTT
                     GESFARRLMERIITPLALEHTAPNPFNPTAFAESGGDAPTFIARMATGYGIDDRRRPV
                     AVPYPALFSSAAGLVSTAREVARYSTALDSGQFLSAATRALAYTPARGAGNRLLPYGL
                     GWFISTVSGHTLVWHYGYWTGNSSLIIKEPDRGLTFVVLTNSDQLSAPFNLGAGDLMS
                     STVAQVFINAFVTGSLAPPTGPIIQELSGSSRIHPESSE"
     gene            6023..9289
                     /locus_tag="VF720_05160"
     CDS             6023..9289
                     /locus_tag="VF720_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7878774.1"
                     /translation="MAPLHIRLRATRQLASISIACLAGTFGFLGGIESAAAHLSIIRQ
                     GAESRGSREAGDRFGRSVAVGDFNGDGFADVATGSPGEDIGTSSNAGAVIINWSNRLG
                     VQHAGAQLLTQASLNNDPEQAQAQFGFALAAGNFDNDAYDDLVVASPYYDAGQADAGA
                     FWYFRGSASGLVAVTWQTQANVGGSNEAGDLFGYSLAVGDFNSDGNDDLAVGGPGEDG
                     NAGAIFQFLGSAFGPIVNGGWFKQSTLGGTNGMGDYFGWSLAAGNMVTPIGTQYDDLA
                     VGAPNKNNTAVTSGGVWVLRGSSSGLRSTSPLYYTAGSFDSQEAGAQFGYSLASGRFF
                     GGSTDGLAVGEPSRTIGGHDNAGRVVYAKGGVAGLQFSTGNYLVQVNAGEVSEANDRF
                     GYAVGAGDLDQVDAYQELMVGAPFEKVDTSIEAGFVAIFFGGPNGPTGQYGRYGLYQS
                     TLNDPVTAGDEFGNAFASGVTDESGRSTIVAGASGDDTDRGMVHVFAPWRQVLNLGTK
                     TSAVWNCEGEFVFTQKPFDEVCIASTTKIMTVLIACERSQLPVGNPKRLGLNNGYIIP
                     QWVREIAGSQYVFYPLERTNLGRLLYCCLYPSGNDAAYAIADMLTGGDAVWTDHLTVV
                     PEFVQEMNDRAAALGMTHTHFSNPAGLDLDGSHHSTAEDMLKLSRAAMENELFRNVAS
                     GQFVDWIREWEYPVGTVRTDWDGLSYGWLDNFQSWDRDGNGLKPGWTPCANDTRCFSH
                     PGSLGRDDVLAATFGSSANEERNGADLIELGLAACGSPLTFSYQGRGHLGHMTATTGN
                     GSSRSLTLPLQEELPGFALELFRQEGTGNTGARLEVERSQELLFQPGQTQSFGVRGAI
                     GEHGEMVVRNLGTNVVSLEVDLPYSSPQVVMLDQYEKHVIPPHVGSAGPVWTVRNITG
                     VTNGQSAHVMVSESYAWELTSIGTGADPKFSEYLLRQGRFDNTRVRIVGRDPNAGSSL
                     YLDLHESGAVSGVGISPEPVTPDAPPVTALSASPNPFTSETRIAFTLGGPGDVVLSIH
                     DIQGRQIVSITRDNLAAGGWTVNWDGRSSDSRPVVAGTYFYRVQVNGRTKSGKIVKVK
                     "
BASE COUNT         1631 a         3121 c         3078 g         1633 t
ORIGIN      
        1 gagatcttcg atgccgcggg acggctggta agggtcctgg ttgcggaaga gcaatccgcg
       61 gggcgccacg agccgatgtg gcagggaacc gacgagagcg ggcgcgacgt gctgtcgggg
      121 atctacttcg ccaggctcac gacgagccac aacggcacgc agactgcatc tcagcggaag
      181 atcaatctgg tgcggtagct acggttcgga tcaacgcgtc accaccagcc gccgcgcatc
      241 ccgtccgcca cccgagcgga tcaccacgta gtagatgccg gacgggacag gccccgcgta
      301 gccgtcgccg ttccactgga tgtcatgcac cccttccgcc agctcgccgt tcagcagctg
      361 ccggacgtga cggcccgaca cgtcgaagac atccaccgat acggacgccg cgcggggaag
      421 tgcgaatctc aacgcggccg gcccgacggt ggggttgggc cacaccgccc ggaatccgaa
      481 accgggcgcg ttcgtcccgg gcctggccgg cgcgggatcc ggaagcccgg aaacggctcc
      541 gttatcgaga tcgagatcca ggatccagag atcgcccagg agcccgtatt gccagccgtc
      601 gcccccgaag acgatgaggc gacgggtgag cggatcccag caggcggtgt ggctgcttcg
      661 ggtcggggga tgcacggtcg gattcggcag ccggctccag ccggccgcgc ccgcatttgt
      721 ggcgccggtc acgaggagga tgtcatcaag ggccatcgtg ccgttggcgt cgatcccgcc
      781 gaacgtgagc aggcgatcgg tggcggcgtc gtagatcccg ccgtgatacg tgcgccccgg
      841 gggcgccggg ttgacgaggg gaagcgtgct ccatgtcggc gttccgccca gaccgtccgc
      901 atgggtcagg tagtgggctt ctccgatcgt ccaggtgcag gccaccgtgc ctccctggac
      961 gatcaaccgg ttggtggcgc tgtcgtaccc cagaacggcc ccgctgcgcg ccgccggcaa
     1021 gggaccggtg gccggcagga gcgaccattc cggagtcccg cccagcccgt tggcatgggt
     1081 caggatccag acatcgttcg aggctcccgc gcaggggtcc aacgtgcgca gtccgccgaa
     1141 aaggatgagt cgattgctcg acgggttgta cgcgatgccg cttccggcac gccggtccgg
     1201 cgtgctgccg ccaggcagca actgcgtcca catcggagtg ccgccgagcc cgttggcgtt
     1261 cgacaggacc catacatcca ggaatccctc cgggcgcgcg gcgctgtaac ccccgtacac
     1321 gatcaatcga ttgttcacgc catcgtaggc ggccgcatgt ccttgccgcg cgagcggcgg
     1381 cgcgtttccc ggcgaaagtg gttgccaggc gggtgtccct ccgcttccgt tggcgttggt
     1441 cagcacccag gtatcgttca actccccggc ggggcccagg cctccgaaga cgatcatgcg
     1501 gttggagaag ccgtcataaa cggcggtgtg cgccagccgc gcggcgggac cggtagccgc
     1561 aacattggtc cacgtctcgg agagcgccgt gaggcgaatc gggtcggaaa cccgggtttg
     1621 cggcacgaaa gccaggtagc ggccgcctcc caggtccgtg ccgttcaccg tggtgaactg
     1681 gccggtgtgg ccgccgcagg tgatcagttc gaaggcctct ccgggctgcg gcatgaaacc
     1741 gctgaccagg gaaacgttca gcgttccagc cagcgtcacg atcccggaaa tcgccagtcg
     1801 atcttggctg gaaccgggaa cggtaccgcc gatttccatt tccacgaccg acgtcgatcc
     1861 caggatgacg tcgccgttca cggtgagcgt ccccggcgag gcgcccggct tgatcgttcc
     1921 atcgttgagg aacgacgtgc cgctcatgtc gcaggtgccg cctcccgaca ggatgccggt
     1981 gggttggttc cggaaggcgc cccccgtgat cgccacaccg gctcccggga agacttccag
     2041 agtgccctgg ttcgagaagt agccggccgg tgtctgcgtg acggtaaacg ttcccccctg
     2101 cacccggatg gtcccgtaat tctggtgtcc ggtgtcgccg ctcgccagga ggacgccgtt
     2161 ctggatggtc acgagccctt cgttgtccag gaaggccagg aggctcgagg tgccctggat
     2221 actcagcgtg ccgaggttca ccaccgagcc ctgcatcgtg gagttcaaca gggagatggt
     2281 gttgccggcg ccgttcgtca cgttgccggt gacatccccg ttctcgatgt cgagcgtgcc
     2341 gctgttggtc aggacgccgc tccccacgat gaggcggccg gacgaaaggg gattcgtctc
     2401 gaggtttccg tggagctcga agccgccggc cacctcgagc gaggtgcccg aaattgtgat
     2461 ggccgtcgcg gcgtgggtca tcaccggcga gttgaggtgc gacgtgccgc tcgaggtcaa
     2521 cgagccctgg aggtcgaggg tgccggcacc ggaaatcgtg cctgagttca agttcaacac
     2581 gtgcgccgtg cccacggcga tgccggtgcc tgtggtcagc gtcgccgagt tcactgtcaa
     2641 tcgtcggctt cccgtgcccc cgctcaggcc gaccgcgcca accgaagcgt tcgaattcac
     2701 actgacggtg tagttcccgc tcaggttgat gatggccacg tccgaaggac cggggacccc
     2761 ggtcggaagc cagttcgacg aggtgtgcca gcttccgttg atggcgcttt tccacgtgta
     2821 attggtggcg aaggccggag cggcaatcag cggcgtcagc gccgccagga tcagaacggg
     2881 gatgagtcgt cgcgggcggt ccatgggtgt ctcctcgttt caggatccac ggccagctcc
     2941 cggcaggtgc ggggcgaggc ggtcccggaa ctccacgcgc agatcgggac cgcggaggac
     3001 caatggaggg gcaaaaggaa tcgaattagc gatgccatgt tccgcaatct cttgacgaat
     3061 aagggcttgg cgccgaatcg acgggcgccg ggcacccgct tcaacgtcga caatgcggtt
     3121 tacgtcgaca cggcccgggc gggacggacc tgcccgcagg ccagtggggc gatggcgatg
     3181 gaaggctccg atccgaatca tgacacctga ccagttgatc cggacccggc gcacccaaag
     3241 gtgcaccatg actctgctct ttcttcttgt catctccctg gctaccgccg gggcggcctg
     3301 cggggagcct ccatccgcgg gagcctcacc ccgcgtcggc ccgaccgacc tggctgaatt
     3361 ctccgcgttc atcgattcga tcatgaccgc cgagatggcc gcggcgcaca ttcccggcgc
     3421 ggctttcgta ttcatccggg accatcgcgt gattcttgcg cgcggctacg ggctggccga
     3481 tgtcgaggct catcgcgccg tggccgtcga ctccacgatt tggcgcgtcg gttcgatctc
     3541 caaggtgttc accgccaccg cggtcatgca actcgtggac cggggcctgg tcgaactcga
     3601 tgctcccatc gatcgctacg tgcacaaggt ttcgatcccg gccacctttc ccgatccggt
     3661 gacggtgcgc cagctgctcg accacacggc cggcttcgac gagatccgcc ccggcaccca
     3721 ggcggccagc cgtgacagcc tgttaccgct ggctgacttc ctgcgccatc ggctcgtacg
     3781 tgtgcgacct ccgggaagga cgatcgccta ttcgacttac ggcatgacac tggccggcga
     3841 gctggtcgag gaagtgtcgg gccagccgtt cgagcgctac atggaagaga acatctggcg
     3901 gccactgggg atgtcgcgga cgtgcatcaa tgttccggaa cgctggcttg gccataccgc
     3961 caggggttac gagctcagcg gcgattccct cgcggtgcag ccctgggagt ggtatcacac
     4021 catcccggca tcctcgatca acgccaccgc ctccgacatg gcccggttcc tgctcgcgca
     4081 ccttgattac ggagccgccg cgtcggagcg cctgttcgac gagcgaaccg ggatggaaat
     4141 gcagcgccag caggtgacga tggacccgcg ccttcccgga ttcgcactcg gcttctacga
     4201 agactacgtc gggaagctcc gcgtcctcga gcatggcggc aacaccgcgg gcttcagcgc
     4261 ggagctgctc ctgatccccg ggcatcacgc cggcttcttc gtcgtgaacc acctcgaggg
     4321 gagtcggctc cgcgacaacc tcaagtgggc gcttctgcag cgctactatc cagccgcccg
     4381 cgaacgacac caagtgcccc ctctgaccac cgcggcgcct tcatcggccg tacgctttgc
     4441 cggacagtac gtgccgacga gcagttgctg gagctgccgc ccggtccggg ccagttcgct
     4501 cttcgatgtc acgtccaatc cggatggatc cctgacgatg gccggtggac gctggattcc
     4561 cgtcgactcc ctcctcttcg tcaacgagaa gggcacgggc tacatcgcgt ttcgcgccaa
     4621 cagctccggc gcgatcactc actgctttcc gggtggattc tggagcttcc agagagttcc
     4681 tgaatgatcg gacccgtcgg cggagcgagc gatccggtga cgaatgcgtt gatgaacacc
     4741 tgggccacgg tggagctcat gaggtcgccg gcgccgaggt tgaacggggc gctgagctga
     4801 tcgctgttcg tgagcaccac gaacgtgagt ccgcggtccg gttccttgat gatgagcgac
     4861 gaatttccgg tccagtagcc atagtgccag accagggtgt gtccgctcac cgtgctgatg
     4921 aaccagccga gaccgtaggg aaggagccga ttcccggcgc cccgtgccgg ggtgtacgca
     4981 agtgcacgcg tggccgcact gaggaactgt cccgagtcga gtgccgtcga gtagcgcgcc
     5041 acctcacgcg cggtcgacac gagaccggcg gcgctgctga acaacgcggg atagggcacg
     5101 gctaccggac gccggcgatc atcgatgccg tatccggtgg ccatcctggc gatgaacgtg
     5161 ggggcatcgc ctcctgattc cgcgaaggcc gtggggttga aagggttggg cgccgtgtgt
     5221 tccagggcaa gcggcgtgat gatgcgctcc atcagccgcc tggcaaagga ttcgcccgtc
     5281 gtccgtccga tgacctggtc gagcaacgcg tagcgatctc cgttgtaatg aaaattctcg
     5341 ccgggaatcc cttcggacgt atgcgagagg aggtggcgga cgcgaatgat gccttgcccc
     5401 ggcagggaga gcccgtagtc ggtaatcgga tcgtcgaggt cgacgagtcg ctgatccacc
     5461 agctgcagga cgatggtgga cgcgaaagtc ttcgtcagcg aggcgaggtg aaacactgtc
     5521 gtgctcgtta ccggtaatcc tgaggcgatg tcggcaacgc ccaacgcacc ttcccacacc
     5581 accgtgtcgt gcctcatgac gatcgccgac atccccggaa tcgccagttc cgcacgaagg
     5641 tcgtcgagtt gcacctcgaa ttccgccagc gtccgggcgg ggggaggaac gtccggatcc
     5701 gcggacgagc accctcccat ggtcaggacc accaggcagg ccagccacaa tttcacccga
     5761 aactcctcca ttggcctttc ggcaaccgtt ggacccgatt gtaccgccct gcacggcaac
     5821 atcgcgctca gcgcccgtcg agccgggtgt atccggacat tgtccggtaa tcgattcgta
     5881 cacttgcact tggcgcacat ggagccccgc gcgtcaaaca gcctgtcccc ctccgagctc
     5941 tttccgtgta tcccgcctgg agcctcccgc ccggcctcaa ccgcggcgtg cgggacacct
     6001 cacctgcagc ccgggagaca tcatggcacc cctccacatc cgccttcggg caactcgcca
     6061 actggcctcg atatccatcg cctgtcttgc cggaacgttc ggcttcctcg ggggcatcga
     6121 aagtgcggcc gcccatctct ccatcatccg gcagggcgcg gaatcgcgcg gatcccgcga
     6181 ggccggggac cgcttcggcc gttccgtggc cgtgggggat ttcaacggtg acggctttgc
     6241 ggatgtggcc accggctcac ccggcgagga tatcggaacc agctcgaacg ccggcgcggt
     6301 catcatcaac tggtcgaacc ggctgggagt gcagcatgcg ggcgcgcagc tcctgacgca
     6361 ggccagtctc aacaacgatc ctgaacaggc gcaggcgcag ttcggctttg ccctggcggc
     6421 cggcaatttc gacaacgatg cctacgacga tctggtcgtg gcctctcctt attacgatgc
     6481 gggtcaggcc gatgccggcg ccttctggta cttccgcgga tcggcctcgg gcctcgtggc
     6541 ggtcacctgg cagacgcagg ccaacgtcgg cggcagcaac gaggcgggcg acctgttcgg
     6601 ctacagcctg gccgtcggcg acttcaattc ggacggaaac gacgacctgg ccgtcggagg
     6661 acccggggaa gacggcaacg ccggcgccat cttccagttc ctcgggtcgg cgttcgggcc
     6721 gatcgtgaac gggggctggt tcaagcagtc cacgctcggc ggcaccaacg gaatgggcga
     6781 ttacttcgga tggagccttg ccgcggggaa catggttacc cccatcggaa cgcagtacga
     6841 cgacctggcg gtcggcgccc cgaacaagaa caacacggcg gttacctcgg gcggcgtctg
     6901 ggtccttcgc ggctccagtt ccggactccg ctccacgagt cctctctatt acacggccgg
     6961 gagcttcgac agccaggaag cgggtgcgca gttcggctac tccctggcgt cgggtcgctt
     7021 cttcggcgga tcgacggacg ggctcgccgt cggggagccg tcgcgcacga tcggcgggca
     7081 cgacaatgcc gggcgcgtcg tctacgcgaa gggaggcgtg gccggtctcc agttcagcac
     7141 cggcaactac ctggtccagg tcaacgccgg cgaagtgtcg gaggccaatg accggttcgg
     7201 ctacgcggtg ggcgcggggg atctggacca ggtcgatgcc taccaggaac tcatggtcgg
     7261 cgcgccgttc gaaaaggtcg acacctccat cgaggcgggc ttcgtcgcca tcttcttcgg
     7321 cggtccgaac ggtcccacgg gtcagtacgg gcgatacgga ctctaccaat ccaccctcaa
     7381 cgatccagtc accgccggtg acgagttcgg caatgcgttc gcgtccggcg ttaccgacga
     7441 atccgggcgc tcgaccatcg tggccggtgc ttccggcgat gacacggacc gggggatggt
     7501 tcacgtcttc gcaccgtggc ggcaggtcct gaaccttgga acgaagactt cggccgtctg
     7561 gaactgcgaa ggggaattcg tctttaccca gaagccgttc gacgaagtct gcatcgcctc
     7621 gaccaccaag atcatgaccg tcctgatcgc ctgcgagcga tcccagcttc cggtcggcaa
     7681 tccgaagcgg ctcggattga acaacggcta catcattcct cagtgggttc gcgagatcgc
     7741 cggctcgcag tacgtgtttt atccgctcga gaggaccaac ctgggacgcc tgctctactg
     7801 ctgtctctat ccctcgggaa acgatgcggc ctacgccatc gccgacatgc tgaccggggg
     7861 agatgccgtc tggaccgacc acctgacggt cgtcccggag ttcgtccagg agatgaacga
     7921 tcgcgccgcg gccctcggca tgacccacac gcacttctcg aacccggccg gtctcgacct
     7981 cgacggcagc catcactcga cggccgaaga catgctcaag ctctcccggg cggccatgga
     8041 aaacgagctc ttccgtaacg tggccagcgg ccagttcgtc gactggatcc gggagtggga
     8101 gtaccccgtg ggcacggtca ggaccgactg ggacggactg agctatggat ggctcgacaa
     8161 cttccagtcc tgggaccgcg atgggaacgg cctgaagccg ggctggacgc cgtgcgccaa
     8221 cgatacgcgc tgtttctccc atccgggcag cctgggccgg gacgatgtcc tggccgccac
     8281 cttcggctcc agcgccaacg aggagcggaa cggcgctgat ttgatcgagc tcggactcgc
     8341 cgcctgcgga agtccgttga ccttctcgta ccaggggcgg ggccacctgg gccacatgac
     8401 cgccacgacc ggcaacggat catcgcgatc gctcacgctg ccgctccagg aagagcttcc
     8461 ggggtttgcc cttgaactgt tccgccagga aggcacgggt aataccggcg cccggctcga
     8521 agtggaacgc tcgcaggaac tcctgttcca gcccggccag acccagtcgt ttggcgtgcg
     8581 gggcgccatc ggggagcatg gggaaatggt ggtccggaac ctcggcacca atgtcgtctc
     8641 gctggaagtg gatcttccgt attcctcacc gcaggtcgtc atgctcgacc agtacgagaa
     8701 acatgtcatt ccgcctcatg tcgggagtgc cggccccgtc tggaccgtgc gcaacatcac
     8761 cggcgtcacg aacggacaat cggcccatgt catggtgtcc gagtcgtacg cctgggagct
     8821 gacctccatc ggcaccgggg cggaccccaa attcagcgag tatctcctcc ggcagggccg
     8881 cttcgacaac acccgggtcc ggatcgtcgg ccgcgacccc aatgccggca gcagcctcta
     8941 cctggatctt cacgaaagcg gcgcggtctc gggtgtcggc atctcaccgg agccggtcac
     9001 cccggacgcg ccgccagtga ccgcactcag cgccagcccc aatcctttca cgtcggaaac
     9061 gcgcatcgcc ttcaccctcg gagggcccgg cgacgtggtt ctctccatcc acgacatcca
     9121 ggggcgtcag atcgtgagca tcacgcgcga caacctggcc gccggtggct ggaccgtgaa
     9181 ctgggacggc cgctcgagcg acagccgccc ggtggtggcc ggaacctact tctaccgcgt
     9241 ccaggtcaac ggtcgcacga aaagcgggaa gatcgtcaag gtaaagtaga tgggcgagcc
     9301 ttacacttgt atggctgtca tgatatggct gaccatctga aaacgaccgt ggagattcca
     9361 gcaggttgcg gcctggatgg aagggccgcg atctgtcccc taccgaacga tcaccagccg
     9421 ctccgtccga gccttctcgt tccaccgcaa ccgataccag tac
//
LOCUS       DASVOD010000244         6601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_329116, whole genome shotgun sequence.
ACCESSION   DASVOD010000244 DASVOD010000000
VERSION     DASVOD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329116"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..733)
                     /locus_tag="VF720_05165"
     CDS             complement(86..733)
                     /locus_tag="VF720_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7878775.1"
                     /translation="MTSDDGSDREHEMLQSFPDFLRQLDRDPELAWSGFHVFAWKLLS
                     AHPPLVFRDLPREGREEIISDIVLACRADNFRLLRGYEDRGVPFAAWVARLARNRALD
                     RMRHDEVVDRHRDSVTRIAARPLSEPADEAVDNRQLLDAVREAIRRLGVRCRLLIEAA
                     ADELKPAEIAHLLGNPIDDNKKISDALRACRRTLRQMLRRHGLEFEGGGMTGRQR"
     gene            complement(730..1752)
                     /locus_tag="VF720_05170"
     CDS             complement(730..1752)
                     /locus_tag="VF720_05170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7878776.1"
                     /translation="MTATARIREQVLATDRLHLILMPTEACNLRCTYCYEDFRMGRMP
                     EDVVAGVERLIERRASALSYLDISWFGGEPLAASDTVIRIMAHARDVAAASAGLTVRS
                     DMTTNAWYLTPTLFERLNDLGVVKYQVSFDGAAAVHDTRRVRKGGGPTFERLWGHLKA
                     MKATDREFTVMLRLHVSADNLGSVAEFLELVRLDLDGDRRFRIYPKLLARWGGARDAA
                     LPVMSAAEGKVVVESLRERAATAGIPLVPLDPADTVCYAARSNAIVVRADGRLNKCTL
                     ALEAAENQVGRLRPDGRFDLDPARLLPWMRGLESGKLGELACPKRGLVGIPTTATITS
                     PALETA"
     gene            complement(1760..2080)
                     /locus_tag="VF720_05175"
     CDS             complement(1760..2080)
                     /locus_tag="VF720_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878777.1"
                     /translation="MDIYRIQLNPGDEASIDVTDKETLEEIEYQMKKHGNERPRVEYL
                     ADEQKLALHFGDADRVDVDVSLLSKLMNPPDGAKTFVEAKVVLVPYRYDPNDPPPPDT
                     MCPC"
     gene            complement(2083..5295)
                     /locus_tag="VF720_05180"
     CDS             complement(2083..5295)
                     /locus_tag="VF720_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing tetratricopeptide repeat
                     protein"
                     /protein_id="HEX7878778.1"
                     /translation="MGRISAFLWFVLLLAPAPATAYDGCASADSVVAVVRGAAVLDTA
                     RLADALLEQGRCRGKTGQADAARGSLDEGLRLVMATTPPDRLREAAFRHEIGAFLINR
                     RDLAGAAPFLEAARDIRTRELGEPHPDTARTRLYLAALLFQSGQRLEAGPEYAKAVAD
                     IEAALGPDHPDCLWAFNLYASYLQATGARAEARVLYDRVLAGRARLELPPDPTEAWLL
                     NNLGILNEEAGDYATAESLYTRSWEIRRDQLGAVPLTARSLVNRGNLFFLRGDYAGAR
                     ALFEEAMRIFKAEGLDRDPDMARCLHRLGMVYFWTGDVVSAGEHLERARTLLDAQGRT
                     ADRLDVFRGLSYCALAGGDARGAERWLRAGIELADDDSTVAVGPRGNLRIDLGRMLAA
                     QHAWSASDRAFADGIGLLESDSAASPVSLGCALYEQGEARLLAGQFREAADDIGASLH
                     LLQPKLEPWHPQLGLCWMGLARAWAGLDHPTAAFETAARAHRLALEQVRNNLALLTER
                     HARLLAASLDEATGLCLATLMGHDAPGPATVLTTWDAVTDTRGLVQRELTARNAAPKG
                     SPAAVQRLRAARTQLANLYLRGPAGEGDDRFGRLLAAAIREREAAERNALGTPSPDRD
                     ASSTTDVPPDADGSVAGLLATLPPAAALVAFALTPDSLGPGAEGAGGRYVAFVGRPGR
                     PPQFVNMASRQEVDVAVDSWRAQAGDASRSLRTDPDAAERRCHEAGQRLRRLAWDPLR
                     AALSGTEVVYLVPAGRLGLVSFAALPDSGNRFLVEGPAAIRVIDHERDCRARPPGSGA
                     GLLALGDPDFGCCVETMAMNDDTRRDAADCRSIAERSFQPLPATREEVGKLADRWQRA
                     APDKGAMEVLLGADAVETAFSSRAAGRRVLHLATHGFSLEGHCRPGSDRGPLAVNAAL
                     GSGLVLSGAAHWSDSRAGTGDGLLTAEEVASLDLSSVDLAVLSACESGAGETTAWEGL
                     IGLRRAFEIAGARHLVVSLWSVGDAEARDWIGAFYQAYLEAGEPPPAAARTAALELLR
                     ARRAAGRSTHPFYWAGFLTVGGGREAMDGSHAAGKQQARP"
     gene            5663..5944
                     /locus_tag="VF720_05185"
     CDS             5663..5944
                     /locus_tag="VF720_05185"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7878779.1"
                     /translation="MTQTQVEFGSIEGTVRNAAGRHLEDVAVFIKSADQPHPDIAALS
                     GRDGTFRLTLLLPGSYVLEAVGAANSPVTIAVRVMSGPPTRIDVVFPPG"
     gene            6051..>6601
                     /locus_tag="VF720_05190"
     CDS             6051..>6601
                     /locus_tag="VF720_05190"
                     /inference="COORDINATES: protein motif:HMM:NF040341.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HEX7878780.1"
                     /translation="MTLARRLPAAAIVFVPLLLLVVLLAACGDDESGSRMDALRRNRA
                     LWQAQGIENYRFMQQKSCFCHSDNLLPTRVEVRQGEPVRAEVVSDGRLVPAHLSLTVD
                     EVFNRVVQAIEGNYEHLDVTYDAERGFPTHLDMDPGNGVVDAGFGVDISELAPLEPGT
                     PCQPPPPLDCSCTAVFPASHYAYT"
BASE COUNT         1027 a         2324 c         2285 g          965 t
ORIGIN      
        1 gcgggttcca tccgtgcccg gcgaacgaga tcattcaacc gttcggcgtc ggcgggatcg
       61 agatcgtcga tgtcgtcggc gccgttcatc gttgcctccc tgtcatgcca ccaccttcga
      121 attccaatcc atggcgacgg agcatctgcc gcagcgtgcg gcgacaggct ctcagggcat
      181 cgctgatctt cttgttgtcg tcgatcgggt tgccgaggag gtgggcgatc tcggccggct
      241 tcagttcgtc ggccgcggcc tcgatgagga gccggcagcg aacgcccagc cggcggatgg
      301 cctcgcgcac cgcgtcgagg agctgacgat tgtcgacggc ttcgtcggcc ggctccgaga
      361 gcgggcgggc cgcgatcctg gtcacgctgt cgcgatgacg atcgaccacc tcgtcgtgac
      421 gcatgcgatc gagcgcccgg ttccgggcca ggcgggccac ccaggctgcg aacggcacac
      481 cgcgatcctc gtagccgcgg aggaggcgga agttgtcggc gcggcaggcc aggacgatgt
      541 ccgagatgat ctcctcgcgc ccttcccggg gaaggtcacg gaagacgagt ggcggatggg
      601 ccgagaggag cttccacgcg aagacgtgga agccgctcca ggcgagttcc gggtcgcgat
      661 cgagctgccg gaggaaatcc gggaagctct ggagcatctc gtgctcgcga tcggaaccgt
      721 cgtcagatgt catgccgtct ccagggcggg cgatgtgatg gttgctgtcg tcggaatccc
      781 gaccaatccc cgcttgggac aggccagctc gccgagcttg ccgctctcca ggccgcgcat
      841 ccagggaagg aggcgggccg gatcgagatc gaagcggccg tcaggcctca agcggccgac
      901 ctggttctcg gcggcctcga gcgcgagcgt gcacttgttg agacgaccgt cggccctcac
      961 cacgatggca ttggaccggg ccgcgtaaca gaccgtgtcg gccggatcga gcggcacgag
     1021 cggaatgccg gccgtggccg cccgctcgcg cagcgactcg acgacgacct tcccttccgc
     1081 ggcgctcatg accgggagcg cggcgtcacg cgccccgccc cagcgggcca acagcttggg
     1141 ataaatgcga aaacgtcgat cgccatcgag atccaagcgg acgagctcga ggaattcggc
     1201 caccgatccg agattgtccg cggaaacgtg caatcgcagc atcaccgtga actcacggtc
     1261 ggtggccttc atggccttga ggtgacccca gagccgttcg aacgtggggc cgcccccctt
     1321 ccggacgcgc cgcgtgtcgt gaaccgcggc cgcgccgtcg aacgacacct ggtacttcac
     1381 gacccccagg tcgttcaatc gttcgaagag cgtgggcgtg agataccagg cattggtggt
     1441 catgtccgaa cggacggtca acccggcgct cgccgccgcc acgtcccggg cgtgcgccat
     1501 gatccggatc accgtgtccg acgccgccag cggctcgccg ccgaaccagg agatgtccag
     1561 gtacgacagc gccgacgcgc gccgctcgat gagccgctcc acgcccgcca cgacgtcttc
     1621 cggcatccgt cccatccgga agtcttcata gcaatacgtg caccggaggt tgcaggcctc
     1681 ggtgggcatg aggatcaggt gcagccgatc ggtggccagc acctgttcgc ggattcgcgc
     1741 cgtcgccgtc atccggacct cagcaggggc acatggtgtc ggggggcggc ggatcgttgg
     1801 gatcgtagcg gtagggaacg agcacgacct tggcctcgac gaacgttttg gcgccgtccg
     1861 gcggattcat gagcttgctg agcagggaga cgtccacatc aacccggtcg gcatcgccga
     1921 agtggagggc gagcttctgc tcgtcggcaa ggtactcgac cctggggcgc tcgttgccat
     1981 gcttcttcat ctggtattcg atttcctcca gggtttcctt gtcggtgacg tcgatcgacg
     2041 cctcgtcccc cggattcaac tggattcgat agatgtccat gttcacggcc tcgcttgttg
     2101 cttgcccgcg gcgtgcgaac cgtccatcgc ttcgcgaccg ccgccgacgg taagaaatcc
     2161 ggcccagtag aacggatgcg tgctgcgtcc tgccgcccgg cgggcgcgca ggagttcgag
     2221 ggccgccgtg cgggcggcgg ccggcggggg ctcccccgcc tcgagatacg cctggtagaa
     2281 ggccccgatc cagtccctgg cctccgcgtc tcccaccgac cagaggctga ccacgagatg
     2341 ccgcgccccg gcgatctcga acgcgcggcg gaggccgatg agcccctccc aggccgtcgt
     2401 ttcgccggcc cccgactcgc aggccgacag gacggccagg tcgacgctcg agaggtcgag
     2461 cgatgccact tcctcggccg tcaggagccc gtcgccggtg ccggcgcggg agtccgacca
     2521 gtgtgccgcg cccgaaagga ccagccccga tccgagcgcg gcgtttaccg ccagtgggcc
     2581 gcgatcggat ccgggacgac aatgcccctc cagggaaaaa ccgtgcgtgg ccaggtgcag
     2641 gacgcgacga ccggccgccc gcgacgagaa tgctgtctcg acggcgtcgg cgccgaggag
     2701 cacctccatg gcgcccttgt cgggcgccgc gcgttgccag cggtcggcca gtttccccac
     2761 ctcctcgcgc gtggcgggca gcggctggaa gcttcgctcg gcgatggacc gacaatcggc
     2821 ggcgtcgcgc cgtgtgtcgt cgttcatggc catcgtctcg acgcagcacc cgaagtcggg
     2881 atctcccagt gccaggagtc cggcgcccga tcccggtggg cgcgcgcggc aatcccgctc
     2941 gtgatcgatc acccggatgg ccgcgggccc ctccaccagg aaacggttgc cggaatcggg
     3001 aagggccgca aacgacacga ggccgaggcg tccggccggc accaggtaca cgacctccgt
     3061 tccggagagc gcggcgcgga gcggatccca ggccagccgg cgcagccgtt gccccgcctc
     3121 gtggcatcga cgttccgcgg cgtcgggatc ggttcgcaac gagcgcgacg cgtcgcccgc
     3181 ctgggctcgc caggaatcga ccgccacgtc gacttcctgc cgggacgcca tgttcacgaa
     3241 ctgcggcggc cgacccgggc gccccacgaa ggccacgtag cgcccgccgg ccccctccgc
     3301 tcccggtccg agcgaatcgg gcgtgagcgc aaaggccacc agcgccgccg caggcggaag
     3361 cgtggccagg agtccggcga cggatccgtc ggcatcggga gggacgtcgg ttgtcgatga
     3421 cgcatcacga tccggggatg gcgtgcccag cgcgttccgc tcggccgcct cgcgttcccg
     3481 gatggccgcc gcgaggagac ggccgaaacg gtcgtccccc tcgccggccg ggccgcgcag
     3541 gtagagattg gccagctgcg tccgtgcggc gcgaagccgc tggaccgccg ccggcgatcc
     3601 cttcggcgcg gcgttccggg ccgtcaactc gcgctggacg aggccccgcg tgtcggtcac
     3661 ggcatcccag gtggtcagca cggtggccgg accgggcgcg tcgtgcccca tcaacgtggc
     3721 caggcagagc ccggtggcct cgtcgagcga cgcggccagg agccgggcgt gccgctcggt
     3781 gagcagggcc aggttgttcc gcacctgctc gagcgccagc cgatgcgcgc gcgccgccgt
     3841 ttcgaacgcg gccgtcgggt gatcgaggcc ggcccacgcg cgcgccagcc ccatccagca
     3901 gagcccgagc tggggatgcc acggctcgag cttcggttga aggagatgga gcgaggcgcc
     3961 gatgtcgtcg gccgcttccc gaaactggcc ggccaggagg cgggcctctc cctgctcgta
     4021 cagggcgcag ccgaggctca cggggctcgc ggcgctgtcg gattccagga gcccgatccc
     4081 gtcggcaaag gcgcgatcgg aggcgctcca ggcatgttgg gccgccagca tccggccgag
     4141 gtcgatccgg aggttgcccc gggggccgac cgccaccgtt gagtcgtcgt cggccagttc
     4201 gatccccgcg cgcagccagc gctcggcgcc ccgggcatcg cccccggcca gcgcgcagta
     4261 cgagaggccg cggaacacgt ccagccgatc ggcggtgcgc ccctgcgcat cgaggagggt
     4321 ccgcgcccgc tcgaggtgct cgccggcgga gacgacatcc cctgtccaga aatagaccat
     4381 gccgaggcgg tgcaggcatc gcgccatgtc gggatcccgg tcgagaccct ccgccttgaa
     4441 gatgcgcatg gcctcctcga agagggcccg cgcaccggcg taatcgcccc ggaggaaaaa
     4501 gagatttccg cgattgacca ggctgcgcgc ggtaagcggc accgctccga gctggtcccg
     4561 acggatttcc cacgaccgcg tgtagagcga ctcggccgtc gcgtagtccc ccgcctcctc
     4621 gttcaggatg ccgaggttgt tcaggagcca cgcttcggta ggatcgggcg ggagttcgag
     4681 gcgggcccga ccggccagca cgcggtcgta gagaacgcgg gcctccgcgc gcgcacccgt
     4741 ggcctgcaga tacgacgcgt acaggttgaa ggcccacagg caatcgggat ggtcaggccc
     4801 cagcgccgcc tcgatgtcgg ccaccgcctt cgcgtattcc gggccggcct ccaggcgttg
     4861 cccggactgg aagaggagcg cggccaggta gaggcgcgtc cgcgccgtgt ccggatgagg
     4921 ctcgccgagt tcgcgggtcc ggatgtcgcg cgcggcttcg aggaagggag cggcgccggc
     4981 caggtcgcgc cggttgatca ggaacgcccc gatctcgtgg cgaaaggcgg cctcccgcag
     5041 acgatcgggc ggtgtcgtgg ccatgacaag ccggaggccc tcgtcgagcg acccgcgcgc
     5101 cgcgtcggcc tggccggtct tcccgcggca gcgcccctgc tcgaggagcg catcggcaag
     5161 ccgggccgtg tcgaggaccg cggcaccccg gacgacggcg acgaccgaat cggcggacgc
     5221 gcacccgtcg tacgccgtag ccggagccgg ggcgaggagc agcacgaacc ataggaacgc
     5281 cgagatacgg cccatcgtgg cgtaatcccc gggggagttc acgaatgtac caaggggacg
     5341 ctcccgcatg gaagcggagg gacgaccggc ggccggaggt ttggggtgcc cccttctcga
     5401 tcgtcccccc gcactcccca aacgagcgag atggccgatt ccggaccgga aagtgcgcca
     5461 aacgcccctc acgaatgccg tccgaaatcc gggggtccgg ccgtttccga gcactccggg
     5521 ccgccgcggg ggctcacccg acgcggtccg gcagctgaca ttcctccatc cggaggggcg
     5581 ggccggcaac acacatgccc ggcacaccgg tccgtccctc cgttattgac tcacaaggcc
     5641 gcggaaaagg agtgggttca ccatgaccca gacgcaagtg gagttcggca gcatcgaggg
     5701 aactgtccgc aacgcggcgg ggcggcacct cgaagacgtg gcggtcttca tcaagtccgc
     5761 cgaccagcct caccccgaca ttgccgcgct ctccggtcgg gacggcacct tccgcctcac
     5821 gctcctcctg cccggctcgt atgtcctcga ggcggtgggg gcggccaaca gcccggtcac
     5881 gatcgccgtc cgggtcatgt ccggcccccc gacccggatc gacgtggtct tcccgcccgg
     5941 gtagccccgg caagccggca accaggcccg ccaccaggcc gccccgggtg cgctagagtc
     6001 attgggtacc tctcgaacac gccccgcgcc ggtgcggaaa ggagaaaccc atgacacttg
     6061 cccgtcgtct tccggccgcc gcaatcgtct tcgtacccct gctgctcctt gtcgtgctgc
     6121 ttgccgcctg cggcgacgac gagtccggca gccgaatgga cgccctccgc cggaatcgcg
     6181 cgctctggca ggcccagggg atcgaaaact accgcttcat gcagcagaaa tcgtgcttct
     6241 gccactcgga caacctcctt ccaacgcgcg tggaggtgcg ccagggcgag ccggtccgtg
     6301 cggaggtcgt gtcggatggg cgcctggtac cggcgcacct ctcacttacc gtggacgagg
     6361 tgttcaaccg ggtggtgcag gccatcgagg gaaattacga acacctcgac gtgacgtacg
     6421 atgccgagcg gggcttcccg acgcatctcg acatggaccc cggcaacggc gtggtcgacg
     6481 cgggtttcgg cgtcgacatc tccgagctgg cgccgctcga gccgggaacg ccctgccagc
     6541 cgccgccgcc gctcgattgc tcgtgcacag ccgtcttccc ggccagtcac tacgcgtaca
     6601 c
//
LOCUS       DASVOD010000245        13006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_746594, whole genome shotgun sequence.
ACCESSION   DASVOD010000245 DASVOD010000000
VERSION     DASVOD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746594"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VF720_05195"
     CDS             complement(<1..930)
                     /locus_tag="VF720_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX7878781.1"
                     /translation="MILYHAARRHPVGGVPSRVAPVPNSPRKVLGNMCGIVAAYGPNA
                     GRRFPEALGAIRHRGPDSEGIHEGSGWAMGIRRLAIIDVAGGDQPIANEDESIWVVVN
                     GEIYNHNALRRDLTARGHIFRTKSDAEVVVHLYEEHGERFVEHLQGMFGLFLATPAGC
                     FVARDRLGIKPLYIARTKESVCIASEIRSLLELPGVGRPAIEDARLADYFLFRYVPEP
                     HTAFSGIDRFPAGHVMKLSTAGESMRRYWQLEPQPSFKGSFDDAVAECEARLDRIVDM
                     HLMSERPVGVFLSGGLDSSVLSALAVRRAQHPLV"
     gene            959..2182
                     /locus_tag="VF720_05200"
     CDS             959..2182
                     /locus_tag="VF720_05200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7878782.1"
                     /translation="MRVLFIDEGHGVGGSTISLYYLMRELAGRGVEPVVWFSAPHAWS
                     ERFRALGVEVIYGRTPAAAPEPALVAQGAPGIAIPAMTGGRSAAPATASGGYRDSDWY
                     RRLSFYKSHWQGHRRGVSDWRGRIEALRPQVVYANNALPLNFAVLTAASELGLGVVCA
                     LRGLQPLRAPHRAFKGKLHAGVAISELVRRHYLAAGFEESRIVRVYNGVDLQDYPWQA
                     PSPQVAKTGGRILFLGRLTGWKGAEVLLKAVAHLVPRRPKLVTVVAGDGPARGDWERM
                     AADLGIARRIRFVGFQPDVMTLLRDSDLLVHASTAPEPFGRVLVEGMAAGVPVVASDH
                     GAAPEIIESGTSGWLARPGNVEALAAAIDEGLSAGARRVTVARAARERVEANFTVNRT
                     ADAVHQLLAGLEIPR"
     gene            2179..3408
                     /locus_tag="VF720_05205"
     CDS             2179..3408
                     /locus_tag="VF720_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878783.1"
                     /translation="MTRGLPGRLRRTSRRSMAALLLVATFAEGCATGGGAGGAAGSAP
                     SWPFHGFTRYDGLSLNLFRDHLFSPGTMAVDPTGTRLFVPAGRSGAMTIVRLPEVSID
                     AVYDFGEGSSFEQIVFRPDGQILYAFASTVEAANLGRVPLARALLEVELGAELGQQPV
                     SLRPGAWSRGVALWPDRGLLYSLDTAGPDLGGGATLTRIDLYSQEVALRRRLGSVPDR
                     VRDDALVYDDRERWILALLSDDEPSSDFDPPDSRTEPGGSFVAWIDPDSLGIRGDVRL
                     DTRLEYLGIIPSSRGIIAVGIDPRDRARGTALIEIDPRMGRDVAWLDLPEPVNDMARA
                     GSRVVFPTPRGLYVVSLDFLSIEGFIAVPFDRPVGVALTPDGGHACVTFDDPDAPGRT
                     ALAVVDVDAGRLVRVIR"
     gene            3405..4409
                     /locus_tag="VF720_05210"
     CDS             3405..4409
                     /locus_tag="VF720_05210"
                     /inference="COORDINATES: protein motif:HMM:NF017885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEX7878784.1"
                     /translation="MNLPAVDPVVELIDRARASGLDPELLAPIFERFVTIARRHAALE
                     RAVLGSVPLSFPAHLLEGSAAVTDPLAGGASLASRERERLGGDPAVLDRLERILEDQG
                     VKVVSLTFPAGSSLLSAFVFHSDTGPAILVDAANFRRERDYALAHAYGHYLADHDPWH
                     AWTCRTGGGGDSIEELRAHGFAGALLVPEAALTDYLAAVPPALKRPVSADLVRQLRVY
                     FEADDRAILGRLLALGHITTSEIAPLMAGLAPVEGDPSADTEGSGAGGMFRGEGHDIA
                     EGLSDRYVTLAVMAHRAGQLDTAGLARALETDESTALAIDGRFRREETGEEEDGQRTH
                     "
     gene            4390..5052
                     /locus_tag="VF720_05215"
     CDS             4390..5052
                     /locus_tag="VF720_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX7878785.1"
                     /translation="MGSVRIEGTGDEIAVGTIFCLGRNYREHAKEMGSEVPTEPVVFL
                     KPTTALLEEGESIRLPSFSRDIHHEVEMVVLIAREGCDIAIADAMEHVGGYGVGLDLT
                     ARDVQAAAKSKGLPWAVAKGFDGSAPVSKFVRAASVPDPHGLDLSLTRNGEVRQSSNT
                     SQMIFRVDQTIAWLSRIFTLTPGDLIYTGTPEGVGPITPGDELVVSLGGIVSAHFRVA
                     GT"
     gene            5049..6374
                     /locus_tag="VF720_05220"
     CDS             5049..6374
                     /locus_tag="VF720_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878786.1"
                     /translation="MKLFRSIFGESAEVLEERGDALRLAGQWREAAWFYGRACRAARA
                     PALRTRLEERAREATHQAFRSMLDEADSLARRRLNREALDVLSEAERFAQGGEDEAMV
                     AARRWSLHDADGPGPAPTAVLTPGHSEDDGMALDGGELGRAFQERLVGRPDSERRRFE
                     SLGPDGRRGFVQLGAGDHAAALESFEKENALRPESWVVRELSAQALEGLGRSEEACQQ
                     YEAAYRRAPDRSILLLEIARILRDRLARPADALERLETAARRHPPGPESLELHLERVF
                     LRHELGRPDDAIQLLTELMHEPSLDRAFLAFNRGGLHEQVGRIDEAERDLKEAVSLAP
                     DNVLYLERVADLIFRARRNAEEALELLEQALAVDAHDFAGRRGGLGNSPDRARLQFKA
                     ARILFVLDRTGEAAGRVEEGLIVSHDPRVHEALLDLRREIRAVERGVNS"
     gene            6399..7097
                     /locus_tag="VF720_05225"
     CDS             6399..7097
                     /locus_tag="VF720_05225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7878787.1"
                     /translation="MFPLRDTYRSATLPVVTTLLVFLNVGIFLYEMVLGPSLTQFMQK
                     FGAVPVDIVSAVSEGNVAGLLSLVTAAFLHGGWLHLIGNMLFLWIFGDNVEDRFGHVR
                     FLLFYLAGGIVASVSHLASDPGSTVPTVGASGAVAAVLGAYWFLFPGARVLALVPLGF
                     FIQTAELPARLFLGIWFVLQIVSGLVDSTVIRNAQGGVAWWAHIGGFLFGWLVAAGLY
                     RTRRRVRHEGTNAA"
     gene            7142..7435
                     /locus_tag="VF720_05230"
     CDS             7142..7435
                     /locus_tag="VF720_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878788.1"
                     /translation="MNTETRRHDEKSAYHRRAKYRDDPIDDEELGWMNEEDGGVLDDW
                     KDVEESADSVETPPMPPTATPSRPRAARRRTGKPSSASGPAGTAKPRRSGGRP"
     gene            7432..8577
                     /locus_tag="VF720_05235"
     CDS             7432..8577
                     /locus_tag="VF720_05235"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX7878789.1"
                     /translation="MRFLLGADFHLGAKLGWLDGQAAARREDLNRAFGRFVDFALRPD
                     QKIDAVLLLGDIFDTHAPAAPLVELVARELARLEAAGLPVAVIPGTHDGYGYADSVWR
                     SGSFPASMTLITSPGVSRTTVTVAGKPVHLYGLAYDAARTPPDPLLALRKDDTAGEGL
                     HIGMIHGSLPASPEWEFRRRDLAIPTAELPQSGLDLLALGHYHNHLEKWFGDTLVTYP
                     GTLEGLKYSESGERSFVTVDFAAEGTSLERTPYPGRRIIDETFDLDRAGTAGTELEKH
                     LDQLAGLDRIVRLTLTGHAPAGFDAATLQARRADKFFHLELDDQSLTVDERLASRLAG
                     ERTVRGLFVRRMQEKIAACEAADRPLAMAALRAGLAEFGADEVADAD"
     gene            8567..10744
                     /locus_tag="VF720_05240"
     CDS             8567..10744
                     /locus_tag="VF720_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7878790.1"
                     /translation="MRIDRLKLDGFGRFDRTIAHDFGPGLNVVLGPNESGKSTLREAI
                     MAILLGFEDKAAEERHRPRGGKRFRGEIDISTVEARWTVQRDFDTHQVVVSRKKGNEA
                     TRLFEGDANPRGRTDDLAAYLDVIQGIIGFTDRGLGGRTFVVGQGGLETAIDETIRQI
                     ISGSQQGDYDTVLTRLEERFFSLTRDNPWSNRKKSRPREIEELGDRLAQIEGRLEKAR
                     ASLRQGGALLTEMESSSENLVALRRSREEKDRLFESYREFFELNRERSELEKRLAVLR
                     DEKEKVKGFEAECRRTEAGIGERLGHFHKAPPDFGEKLKNLGTLDRDIAALADEHKRR
                     QAILAANTWSGQTKRNFIIAAIVTIVTSLAMWGAGVGWASLAIGLVVGVLSVVGLYLL
                     GNRKERERMKMEASVLEMEDRLELQKGEMSRLEADVRPVIGSQRMTEALEEWREYREL
                     ADLLSRHEQIRDSHRPLAEVEGDYDDVFQKLKLADARARDLIAQSPYLANADQNLEDA
                     SLQVERLRREREALLADEQRETDRLEDLKLRRARQEGGQVDDAESLEDELAESRERLA
                     ALRFRRDAFRMAVDVLKECVTEFQEGHLERLAGDAGTHLARITAGRWTRVALDRNFVP
                     RVVDAEGQTFEPEALSQGTRDQLHLSLRLALIDGIFGDAGPALVLDDALVHCDIERLA
                     TIRKMLEQEVARGRQVLLFSHDPAYAAWGATVIRLKGAAPALV"
     gene            10761..11513
                     /locus_tag="VF720_05245"
     CDS             10761..11513
                     /locus_tag="VF720_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878791.1"
                     /translation="MLRAQGLTRRYGSLLALDRLNLDIGPGQIFGLLGPNGAGKTTTI
                     KLMTGLLRPSEGSVQVGSYDLVTQPREARRLIGYVADDPHLYDKLTPVEFLRFSAELY
                     GVGAQSLPERTQRLLHLFELDGRGDYLIEGLSHGMKQKVALAGALVHEPRVLFLDEPT
                     VGLDPKGARTLKQILRHLAGKGVTVILSTHILEIAERLCDRVAILAKGRLLATGTLAE
                     LKERIGDKSLEEIFLEMTGGNDSADIAAALDP"
     gene            11543..>13006
                     /locus_tag="VF720_05250"
     CDS             11543..>13006
                     /locus_tag="VF720_05250"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878792.1"
                     /translation="MSFARLLGWQLRIAWAGMVRGRKARIGQRILLTSVAVVWLALLG
                     AGIWLALSEAISRTPEARWLLDLTLYSAWLGALAMVIFFNLGFLLHVVFFSRDLEFLM
                     VTPVSARVVLGARFVEGMWSNLLLNLFLSMPAVVAVGLVANAPIAWYLLFVVAQISFL
                     AIPVALTYLVGIPLARWVSTQRLRALFSILGFGLALFLWSLPQLAPSRLRSAAEWDRL
                     MTQGIDILRLFESPAALLWPSTWAASAQVEALRGHWPAALGRLAVLVGVAGLLVALSV
                     RLAANSYRHGWIRMASVSANRPVRPERFTFLLRLVPGDWRPLMWKDLTIVARDFRLSF
                     QLYSLGILMCLFPFMTLATSSSEAAVMAPLTALSCAVGAAVVVGSQAGIMLVPMEGRS
                     AWRLLAAPISVRHLALIKWLSATTLTLPVVVGQAALLILAFHRSFSDAFTGGLISLGA
                     ALIGAGFGTWTGTSFANFEWDHPRRMITPGAQIIWGLG"
BASE COUNT         1924 a         4480 c         4491 g         2111 t
ORIGIN      
        1 cacgaggggg tgctgggcgc gccgcacggc cagcgccgag agcaccgatg agtcaagccc
       61 tcccgacagg aagacgccga caggccgttc ggacatgagg tgcatgtcga cgatccggtc
      121 gaggcgggcc tcgcactcgg ccaccgcgtc gtcgaacgat cccttgaagg atggctgcgg
      181 ctcgagctgc cagtagcggc gcatcgattc gccggcggtg gagagcttca tgacatgccc
      241 ggcggggaag cgatcgatcc cgctgaaggc cgtgtgcggc tcgggcacgt agcggaagag
      301 gaagtagtcg gccaggcgcg cgtcctcgat cgccggccgc ccgacgcccg gcaactcgag
      361 gagtgaccgg atctcggagg cgatgcagac cgactccttc gtgcgggcga tgtagagcgg
      421 cttgatgccg agccggtcgc gagccacgaa gcatcccgcg ggcgtggcga ggaagaggcc
      481 gaacatcccc tggagatgct ccacgaagcg ctccccgtgc tcctcgtaga ggtgcaccac
      541 gacctcggca tccgacttcg tgcggaagat gtggccgcgg gccgtaaggt cgcggcggag
      601 ggcgttgtgg ttgtagatct ccccgttcac gacgacccag atcgactcgt cctcgttggc
      661 gatcggctgg tcgccgccgg ccacgtcgat gatggcgagt cggcggatgc ccatggccca
      721 gcccgacccc tcgtggattc cctccgagtc ggggccgcgg tggcggatcg cgccgagcgc
      781 ttccgggaag cgccgcccgg cgttcgggcc gtaggcggcc acgatgccgc acatgtttcc
      841 caggaccttt cgaggggagt tggggacggg cgccacgcgg gaggggacgc cgccgaccgg
      901 gtgtcggcgc gccgcatggt agaggatcat cggcatcgct gctagacttt cggcgactat
      961 gcgagtcctc ttcatcgacg aaggtcacgg cgtggggggc tcgacgatca gtctctatta
     1021 cctgatgcgc gagctggccg ggcgcggcgt ggagccggtg gtctggtttt ccgcaccgca
     1081 cgcctggagc gagcggttcc gcgccctcgg cgtggaggtg atctacggcc gcacgccggc
     1141 ggcggccccc gagccggccc tggtggcgca gggggcgccg ggcatcgcca tccccgcgat
     1201 gaccggtggc cgctccgccg cgccggcaac cgcgagcggc ggttaccgcg actccgactg
     1261 gtaccgccgc ctctccttct acaagagcca ctggcaggga catcggcgcg gcgtttcgga
     1321 ctggcgaggg cgcatcgaag cgcttcggcc gcaggtggtt tacgccaaca acgcgcttcc
     1381 gctgaacttt gccgtcctca cggcggcctc ggagctggga ctcggcgtcg tctgtgcgct
     1441 gaggggcctg caaccgcttc gagccccgca ccgggccttc aagggaaagc tccacgcggg
     1501 cgtagccatc tccgaactgg tgcgccgcca ttacctggcg gcaggcttcg aggagtcgcg
     1561 catcgtccgc gtgtacaacg gggtcgatct ccaggattac ccatggcagg cgccttcgcc
     1621 gcaggtggcg aagacgggag gtcggattct cttcctgggc cgccttaccg ggtggaaggg
     1681 ggccgaggtg ctcctcaagg ccgtagcgca cctggtaccc cggcggccga agctggtcac
     1741 cgtggtggcg ggagacgggc cggcccgcgg cgattgggaa cggatggccg ccgatctcgg
     1801 catcgcccga agaatccgct tcgtgggatt ccagcccgac gtgatgacgc tcctccgcga
     1861 cagtgacctc ctggtgcatg cttccacggc cccggagccg ttcgggcgcg tgctcgtgga
     1921 ggggatggcg gccggcgtgc cggtggtggc ctccgaccat ggcgccgctc cggaaatcat
     1981 cgagtcgggg acgagcgggt ggctggcgcg ccccggaaac gtggaagcgc tggcggcggc
     2041 catcgacgag ggactgagcg ccggggcgcg gcgcgtcacg gtggccaggg ccgcccggga
     2101 acgcgtggaa gccaacttca ccgtaaaccg cacggcggac gcggtgcatc agctcctggc
     2161 cggactcgaa atcccacgat gacgcgcggg cttccgggtc ggttgaggcg gacctcgcgg
     2221 cgatcgatgg cggcgctgct tctcgtggcc actttcgcgg aaggctgcgc gaccggcggc
     2281 ggtgcgggtg gggcggccgg ctccgccccg agctggccgt tccacgggtt cacgcgatac
     2341 gacggtcttt cactgaacct ctttcgcgac cacctcttct cacccggcac gatggccgtc
     2401 gacccgaccg gcacgcgcct cttcgttccg gccggtcgct cgggagccat gaccatcgtg
     2461 cggctccccg aggtgtcgat cgacgccgtg tacgatttcg gcgaggggag ttcgttcgag
     2521 cagatcgtct tccgacccga cggccagatc ctctacgcct tcgcgagcac ggtggaggcg
     2581 gccaacctcg ggcgcgtgcc gctggcccgg gcactgctcg aagtggaact tggcgcggag
     2641 ctggggcagc aaccggtgtc gctccggccg ggcgcctggt cgcgcggcgt ggccctctgg
     2701 cccgaccggg ggctcctcta cagcctggac accgccggcc ccgacctcgg tggaggagcc
     2761 acgctcaccc gcatcgacct gtacagccag gaagtggcgc tccgccggcg gctcggcagc
     2821 gtgccggatc gggtgcgtga cgacgccctg gtgtacgacg atcgcgagcg ctggatcctg
     2881 gcgctcctct ccgatgacga accctcctcc gacttcgatc cacccgactc gcggaccgag
     2941 ccgggtggca gcttcgtggc ctggatcgat cccgattcgc tcggcatccg cggggatgtc
     3001 cgcctcgata cgcggctcga atacctgggg atcatcccct cctcgcgcgg gatcatcgcg
     3061 gtggggatcg acccccggga ccgcgcgcgc ggcacggcac tcatcgagat cgacccacga
     3121 atgggacgtg acgtcgcgtg gctcgacctc cccgagccgg tgaacgacat ggccagggcc
     3181 ggcagccgcg tggtcttccc gacgccccgt ggcctctacg tggtgtcgct cgactttctc
     3241 tcgattgagg ggttcatcgc ggtgccgttc gaccggccgg tcggcgtggc gctcacgccg
     3301 gatggtggac acgcctgcgt gacgttcgat gatcccgacg cgcccggccg gacggcgctg
     3361 gccgtcgtcg acgtggatgc gggtcgactg gtccgggtga ttcgatgaac ctcccggccg
     3421 tcgaccccgt ggtcgaactg atcgaccggg cacgggcctc cggcctcgac ccggagcttc
     3481 tcgcgccgat cttcgagcgc ttcgtcacga tcgcgcggcg tcacgcggcg ctggagcggg
     3541 ccgtgctggg ttcggtgccc ctctcgttcc cggcgcacct gctcgaagga tctgcggccg
     3601 tcaccgatcc cctggcaggg ggtgcgtccc ttgcctcgcg cgagcgcgag cgcctgggcg
     3661 gcgatccggc ggtgctggac cgcctcgagc ggatcctcga ggaccagggg gtcaaggtcg
     3721 tgtcgctgac gttccccgcc ggttcgtcgc tcctgtccgc ttttgtgttc cactctgata
     3781 ccggccccgc catcctggtg gatgccgcga atttccgtcg ggagcgcgac tatgccctgg
     3841 cgcacgccta cgggcactac ctcgcggatc acgacccgtg gcacgcgtgg acttgccgaa
     3901 ccgggggtgg tggtgattcc atcgaggagt tgcgggccca cgggttcgcc ggagcgctcc
     3961 tcgtgccgga agcggcactg accgactacc tggcggccgt gccgccggca ctgaagcgcc
     4021 cggtttcggc cgacctggtc cggcagctgc gcgtctactt cgaggcggat gaccgcgcca
     4081 ttctcggccg tctgctggcc ctggggcaca tcaccacctc ggagatcgcg ccgctcatgg
     4141 ccgggctggc accggtcgag ggtgacccgt cggccgacac ggaaggatcg ggcgccggag
     4201 ggatgttccg cggcgaaggg cacgatattg ccgaggggct ttcggaccgg tacgtgacgc
     4261 tggccgtcat ggcgcaccgc gccggccagc tcgatacggc gggcctggcc cgcgcgctcg
     4321 aaacggacga atccaccgcg ctggcgatcg atggccgatt ccggcgcgag gagacggggg
     4381 aggaggagga tgggcagcgt acgcattgaa ggaacgggcg acgagatcgc cgtcggcacc
     4441 atcttctgcc tgggccgcaa ttaccgggag cacgcgaagg aaatggggag cgaggtgccc
     4501 accgagccgg tggtcttcct caagccgacc acggcgctgc tcgaggaagg ggaatcgatc
     4561 cgcctcccct ccttttcgcg cgacatccat cacgaggtgg agatggtcgt gctgatcgcc
     4621 cgcgaagggt gcgacatcgc gatcgccgac gccatggaac acgtgggggg ttacggcgtg
     4681 gggctcgacc tgaccgcccg ggacgtgcag gccgcggcca agtcgaaggg gctgccgtgg
     4741 gccgtggcca aggggttcga cggctcggcg ccggtctcga aattcgtgcg ggccgccagc
     4801 gtaccggatc ctcacgggct cgatctctcg ctgacacgga acggcgaggt gcgccagtcg
     4861 tccaacacgt cgcagatgat cttccgggtc gaccagacga tcgcctggct ttcccgcatc
     4921 ttcacgctca cgcccggcga cctcatctac acggggacgc ccgagggcgt ggggccgatc
     4981 acgcccggtg acgaactggt ggtctcgctt ggcggcatcg tttcggccca cttcagggta
     5041 gccggcacat gaagctcttc cgttcgatct tcggcgagtc ggccgaggtg ctcgaggagc
     5101 ggggtgatgc cctccgcctg gcgggccagt ggcgcgaagc cgcgtggttt tacggccggg
     5161 cctgccgggc tgcccgtgcc cccgcactgc gcacccggct cgaggagcgc gcgcgcgagg
     5221 ccacgcacca ggccttccgc tcgatgctcg acgaggccga cagcctggcc cgccgccgcc
     5281 tcaaccgcga ggcgctcgat gtcctctccg aggccgagcg cttcgcccag ggtggggagg
     5341 atgaggcgat ggtggccgcg cgccggtggt cgctccacga tgccgatggc ccgggcccgg
     5401 ctcccactgc cgtcctcacg cctggacatt cggaggatga cggaatggcg ctcgacggcg
     5461 gcgagctcgg tcgggccttc caggaacggc tggtcggccg gcccgacagc gagcggcgcc
     5521 gcttcgaatc gctcgggccc gatggtcggc gggggttcgt gcagctcggt gccggcgatc
     5581 acgccgccgc gctcgagtcg ttcgagaagg agaatgccct ccgccccgag agctgggtgg
     5641 tgcgcgagct ctcggcccag gcgctggagg ggctcgggag gagcgaggag gcctgccagc
     5701 agtacgaggc ggcctaccgg cgggcgccgg accgctccat cctcctcctc gaaatcgccc
     5761 gcatcctgcg cgaccgcctg gcccgcccgg ccgacgcgct cgaacggctc gaaaccgcgg
     5821 cgcggcgcca tccccccgga ccggaatcgc tcgagctgca ccttgaacgt gtcttcctcc
     5881 ggcacgagct cggtcgcccc gacgacgcca tccaacttct caccgagttg atgcacgagc
     5941 cctcgctcga ccgcgccttt ctggcgttca accgcggcgg actccacgag caggtcggtc
     6001 ggatcgacga ggcggagcgc gacctgaagg aggccgtctc gctggctccc gacaacgtgc
     6061 tctacctgga gcgcgtagcc gacctgatct tccgagcccg gcgcaacgcg gaggaggccc
     6121 tcgaactgct cgaacaggcg ctggcggtcg acgcccacga cttcgccggc cggcgggggg
     6181 gtctcggcaa ttccccggat cgggcccgcc tccagttcaa ggcggcccgc attctcttcg
     6241 tcctcgaccg gacgggggaa gcggcgggac gggtggagga ggggctgatc gtgagtcacg
     6301 acccccgggt tcacgaggcc ctgctcgatc tccggcgcga gatcagggcg gtggaacgcg
     6361 gcgtaaacag ctaacctgta aagacttggc gctgcgctgt gttcccgctc cgcgatacgt
     6421 accggtcggc caccctgccg gtggtcacca cgctcctcgt gttcctcaac gtggggatat
     6481 tcctgtatga aatggtcctg gggccctcgc tcacccagtt catgcagaag ttcggggctg
     6541 tcccggtcga tatagtcagt gccgtgtcgg aagggaacgt ggcggggctc ctgtcgctcg
     6601 tcacggccgc cttcctgcac ggtggatggc tacacctgat cggcaacatg ctgttcctct
     6661 ggattttcgg ggacaacgtg gaggaccggt tcgggcacgt ccggtttctc ctgttctacc
     6721 tggccggggg gatcgtcgcc agcgtctcgc acctggcgag cgatccaggt tccacggttc
     6781 cgaccgtggg agccagtgga gcggtggcgg cggtgctcgg cgcgtactgg ttcctcttcc
     6841 ccggcgcacg cgtgctggcg ctggtgccgc tcggtttctt cattcagacg gcggaacttc
     6901 cggcccggct ctttctcggg atatggttcg ttctgcaaat cgtgtcgggg ctggtcgatt
     6961 ccacggtcat ccggaacgcc caggggggcg tggcctggtg ggcgcacatc ggcgggttcc
     7021 tgttcggctg gctggtggcg gcggggttgt accgcacgcg ccggcgggta cgccacgagg
     7081 gaacgaacgc agcatgagtg agcggacgga tcggaaaccg aaaccgcggg tccacccgaa
     7141 gatgaatacc gagacccggc gtcacgacga gaaatcggcc tatcaccgca gggccaagta
     7201 ccgggacgac ccgatcgacg acgaggaact gggctggatg aacgaggagg atggcggggt
     7261 gctggatgac tggaaggatg tcgaggagtc ggcggattcg gtcgaaacgc cgcccatgcc
     7321 gccaacggcc acgccttcaa ggccccgtgc cgcccgccgg cgtacgggca agccctcatc
     7381 cgcgtccggg ccggcaggta cggccaagcc gcgccgctcc ggggggcgac cgtgaggttt
     7441 ctgctcggtg ccgacttcca cctgggcgcg aagctgggat ggctcgacgg ccaggcggcc
     7501 gcccgtcgcg aagacctcaa ccgcgccttc ggccgtttcg tcgatttcgc cctgcgcccg
     7561 gaccagaaga tcgacgccgt cctgctcctc ggcgacattt tcgacaccca tgccccggcc
     7621 gcccccctcg tggagctggt ggcgcgggaa ctggcgcggc tcgaggcggc cgggctcccg
     7681 gtggcggtga tccccggcac gcacgacggc tacggatacg ccgacagcgt gtggcgctcg
     7741 gggagctttc ccgcgtcgat gacgctcatc acctcgcccg gcgtgtcacg caccacggtc
     7801 accgtggcgg gcaagccggt gcacctgtac ggcctggcct acgacgcggc ccgcacgccg
     7861 cccgatcccc tcttggccct gaggaaggac gacacggccg gagaggggct ccacatcggc
     7921 atgatccacg ggtcgttgcc ggccagcccc gagtgggagt tccgccgccg cgacctggcc
     7981 atcccaactg ccgagcttcc gcagagcggg ctcgacctgc tcgccctggg tcactatcac
     8041 aatcacctgg agaagtggtt cggcgacacg ctggtcacct atcccggcac cctcgaggga
     8101 ttgaagtact cggagtcggg cgagcgctcg ttcgtcaccg tcgactttgc cgccgagggg
     8161 acctcgctgg agcggactcc ctacccgggg cgccgcatca tcgatgaaac cttcgacctc
     8221 gaccgggccg gcacggccgg taccgagctc gagaagcacc tggaccagct ggccgggctc
     8281 gatcgaatcg tacgccttac gctgaccggc catgcgccgg ccgggttcga tgccgccacg
     8341 cttcaggcgc gccgggccga caagttcttc catctcgaac tcgacgacca gtcgctgacc
     8401 gtcgacgagc ggctggcctc gcgcctggcc ggggagcgga ccgtccgggg cctcttcgtg
     8461 cggcggatgc aggaaaagat cgccgcgtgc gaagccgcgg atcgtccact cgccatggca
     8521 gccctccggg ccggtctggc cgagttcggc gccgatgagg tggccgatgc ggattgatcg
     8581 cctgaagctc gacggcttcg gccgtttcga ccggaccatc gcgcacgact tcggcccggg
     8641 cctcaacgtg gtcctcggcc ccaacgaaag cgggaaatcc acgcttcgcg aagccatcat
     8701 ggccatcctg ctcggcttcg aagacaaggc cgccgaggag cggcaccgcc cgcgcggcgg
     8761 caagcgcttc cgcggcgaga tcgatatttc gaccgtggaa gcgcgctgga ccgtgcagcg
     8821 cgatttcgac acgcaccagg tggtcgtctc ccggaagaag gggaacgagg ccacccgtct
     8881 cttcgaggga gacgccaatc cgcgcggtcg caccgacgac ctggccgcct acctcgacgt
     8941 gatccagggg atcatcggct tcaccgatcg cggcctcggc gggcggacct tcgtggtcgg
     9001 ccaggggggc ctggaaaccg ccatcgacga gacgatccgc cagatcattt ccggcagcca
     9061 gcagggggac tacgacaccg tgctgacccg gctcgaggaa cgcttctttt cactcacccg
     9121 ggacaacccc tggagcaacc ggaaaaagag ccgcccgcgc gagatcgaag agctgggcga
     9181 ccgccttgcc cagatcgaag gaaggttgga gaaggcccgt gcgtcgctgc ggcagggagg
     9241 cgcccttctc accgagatgg aatcgtcgag cgaaaatctg gtggccctcc ggcgctcccg
     9301 cgaggagaag gaccgcctct tcgagagcta ccgcgagttc ttcgagctca accgcgagcg
     9361 gagcgagctg gaaaagcgcc tggccgtcct acgggacgaa aaggaaaagg tgaaggggtt
     9421 cgaggccgaa tgccgtcgga cggaagccgg catcggcgaa cgcctcggtc acttccacaa
     9481 ggcccctccc gatttcggcg agaagctcaa gaacctgggc acgctcgacc gcgacattgc
     9541 cgctctggcc gacgaacaca agcggcgcca ggcgatcctc gcagccaata cctggtcggg
     9601 gcagacgaag cgcaacttca tcatcgccgc gatcgtcacg atcgtcacgt cgctggccat
     9661 gtggggggcg ggcgtcggct gggccagcct ggccatcgga ctcgtcgtcg gcgtgttgag
     9721 cgtggtcggt ctctacctgc tcggcaatcg caaggaacgc gagcggatga agatggaagc
     9781 ttccgtcctc gagatggaag accgcctcga gctccagaag ggggagatgt cgcgcctcga
     9841 ggccgatgtc cgccccgtca tcggctcgca acgcatgacc gaagcactgg aagagtggcg
     9901 cgaataccgc gagctggccg atctcctctc ccggcacgag cagatccgcg acagtcatcg
     9961 cccgctggcc gaggtggagg gggactacga cgacgtcttc cagaagctga agctcgccga
    10021 cgcccgggcg cgtgacctga tcgcgcaatc cccctacctg gccaatgccg accagaacct
    10081 ggaagacgcc tccttgcagg tggagcgcct ccggcgcgaa cgggaggcgc tcctggccga
    10141 cgagcagcgg gagaccgatc gcctcgaaga cctgaagctc cgcagggccc ggcaggaagg
    10201 gggccaggtg gacgacgccg agagcctgga agacgagctg gccgagtcgc gcgagcgcct
    10261 ggccgctctc cgcttccgcc gggatgcctt ccggatggcg gtggatgtgc tcaaggagtg
    10321 cgtgactgaa ttccaggagg ggcatctcga gcgactggcc ggggatgccg gcacccatct
    10381 tgcccggatc accgccggac ggtggacacg cgtggcgctc gatcgtaatt ttgtcccccg
    10441 ggtcgtggat gccgaggggc agaccttcga accggaagca ctgagccagg ggacgcggga
    10501 tcaactccat ctctcgctcc ggctggccct catcgacggg atcttcggcg acgccggacc
    10561 ggccctcgtg ctcgacgacg cgctggtcca ctgcgacatc gaacgcctgg cgaccattcg
    10621 gaagatgctc gagcaggaag tggcgcgcgg gcggcaggtg ctccttttca gccacgatcc
    10681 ggcgtacgcc gcgtggggcg ctacggtcat tcgcctgaag ggggcggctc ccgcgctcgt
    10741 ctgatacgct cccggcatct atgctgcgcg cgcagggtct cacccgtcgt tacggttcgc
    10801 tgctggccct cgaccgcctg aaccttgata tcggccccgg ccagatcttc ggcctcctcg
    10861 gcccgaatgg cgccggcaag accaccacga tcaagctgat gaccggcctc ctccgcccga
    10921 gtgaaggttc ggtgcaggtg ggaagttacg acctcgttac gcagccgcgc gaggcgcgac
    10981 gcctgatcgg ctacgtggcc gatgatcccc acctctacga caagctcacg ccggtggaat
    11041 tcctccgctt cagcgcggag ctgtacggcg tcggcgccca atcgcttccc gagcgcaccc
    11101 aacgcctcct gcacctcttc gaactcgacg gtcgggggga ctacctgatc gaggggctct
    11161 cccacgggat gaagcagaag gtggcgctgg cgggcgccct ggtgcacgag ccgcgcgtcc
    11221 tcttcctcga cgaaccgacg gtcggcctcg atccgaaggg ggcccgcacg ttgaagcaaa
    11281 tcctccgcca cctggcgggg aagggggtga cggtcatcct ctccacccac atcctcgaaa
    11341 ttgccgagag gctctgcgac cgcgtggcca tcctggccaa ggggcgcctc ctggccaccg
    11401 gcacgctggc cgaactcaag gaacggatcg gcgacaaaag cctcgaggaa atcttcctcg
    11461 aaatgacggg cggcaacgac tcggccgaca tcgcggccgc gctcgatccg tgagtgccgc
    11521 ccggccggac atcgaccgcc cgatgtcgtt tgcccgcctc ctcggctggc agctccggat
    11581 cgcctgggcc ggcatggtgc gggggcgcaa ggcgcgcatc ggccagcgga tcctcctcac
    11641 gtccgtggcg gtggtgtggc tggcgctcct cggcgccgga atctggctgg cactgtcgga
    11701 ggccatcagc cgcacgccgg aagccaggtg gctgctcgac ctcacgctct actcggcctg
    11761 gctcggcgcg ctggccatgg tcatcttctt caacctgggc ttcctgcttc acgtcgtctt
    11821 cttctctcgc gacctggagt tcctgatggt gacgccggtc agcgcgcgcg tggtcctcgg
    11881 cgcccgcttc gtcgagggga tgtggtcgaa cctcctgctc aatctcttcc tctcgatgcc
    11941 ggccgtcgtg gcggtcggcc tggtggccaa cgccccgatc gcgtggtacc tgctgtttgt
    12001 cgtggcgcag atttcattcc tggcgattcc cgtggcgctc acctacctgg tcggaatccc
    12061 gctcgcacgg tgggtgtcga cgcaacgcct gcgcgccctc ttctcgatcc tcggcttcgg
    12121 cctggcgctc ttcctatggt cgctgccgca gctcgcccct tcacgcctcc gttcggccgc
    12181 cgagtgggac aggttgatga cgcaggggat cgacatcctc cgcctcttcg aatcaccggc
    12241 agcgctcctc tggccctcca cgtgggccgc ctccgcgcaa gtcgaggcgc tgcgcggtca
    12301 ctggccggcc gcgttgggac gcctggccgt gctggtcggc gtggccggcc ttctcgtggc
    12361 cctttcggtt cggctggccg ccaactcgta tcgacacggt tggatccgga tggcgtcggt
    12421 atcggccaac cggccggtac gccccgaacg cttcaccttc ctgctccgcc tcgtcccggg
    12481 cgattggcgt ccgctcatgt ggaaggacct cacgatcgtg gctcgtgatt tccgcctgtc
    12541 gttccagctc tacagcctcg gcatcctgat gtgcctcttc ccgttcatga cgctggccac
    12601 cagctcgagc gaagcggccg tgatggcgcc cctcaccgcc ctctcctgcg ccgtcggcgc
    12661 cgcggtggtg gtcggcagcc aggccggcat catgctggtg ccgatggaag gacgctcggc
    12721 ctggcggctc ctggccgcgc ccatctccgt ccggcacctg gccctcatca agtggctctc
    12781 cgccaccacg ctcacgcttc ccgtcgtggt gggacaggcg gccctcctca tcctggcatt
    12841 ccatcgctca ttcagcgacg ccttcaccgg cggcctcatt tccctcggcg ccgccctcat
    12901 cggcgccggc ttcggcacct ggaccggcac cagtttcgcc aacttcgagt gggatcaccc
    12961 ccgccgcatg atcacccctg gagcccagat catctggggc ctcggc
//
LOCUS       DASVOD010000246         2227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_590382, whole genome shotgun sequence.
ACCESSION   DASVOD010000246 DASVOD010000000
VERSION     DASVOD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590382"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VF720_05255"
     CDS             <1..484
                     /locus_tag="VF720_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878793.1"
                     /translation="TGLVRNITALSTLTVLSLLAIVTLVGCGTDVSTAPEFVNIESDD
                     GGYQPGELPLPGHKGLIEAPRGLAIEDITASGAILTWNPASAGLTAQISLNGVRIAEV
                     DATTGKFTDKLAKRPGKYHYAVCFWNGERSGGMRVVDGQVKSTPADDDRRDDRSEDGR
                     "
     gene            complement(683..1228)
                     /locus_tag="VF720_05260"
     CDS             complement(683..1228)
                     /locus_tag="VF720_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014855765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX7878794.1"
                     /translation="MTPDTSPDTSPGKGKKILVFVEHYYEDLELWYPRIRLEEEGYQV
                     VVAGPERKTYPGKNGYPCPADAAIAEIDADAFAGLVIPGGWAPDRLRRYPRVLELVQE
                     FDRAGKTIGMICHAGWVPISARILKGKTVTGVSAIKDDLENAGATFVDRSVVVDGHLV
                     SSRTPADLPDFMKAMIANLAR"
     gene            1347..>2227
                     /locus_tag="VF720_05265"
     CDS             1347..>2227
                     /locus_tag="VF720_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878795.1"
                     /translation="MNNQPRIRPIRAALSALPILALLLYGLLVSSPDGRAARGSGATV
                     TADPRVELAALLARSAGDFGAPWPTDLPTGSVAQLDRTVESKFTALSTLPAPRRVGMY
                     RSAGLTAEVLTTIFLNRGPWPEMKREAGEPYDTEAITRLPGGPTFLARREIFTPGFID
                     SLVEETREFASAAGFENVWSSSQPILLERAESIENDPTLGHLTARLADFLGEPSKAEP
                     WIVPTRFGTWRTPFGVPVNDGDRIVVVDRAESAGKLTPETSLSWICVREFSRPTVERL
                     ARANKDRITGLAGYWTYL"
BASE COUNT          378 a          773 c          730 g          346 t
ORIGIN      
        1 gaccggcctc gtccggaaca tcaccgccct gtcgaccctc accgtcctgt cgctcctcgc
       61 catcgtcacg ctcgtcggtt gcggcaccga tgtgtccacg gcgccggaat tcgtcaacat
      121 cgagagcgat gatggcggat accagccggg ggaattgcct ctccccggac acaagggact
      181 catcgaagca ccgcgcggcc tggcgatcga ggacatcacg gcgagcggcg ccatcctcac
      241 ctggaatccg gcaagcgcgg ggctcacggc ccagatcagc ctgaacggcg tccggattgc
      301 cgaggtggat gccacgaccg gcaagttcac ggacaaactg gcgaaacgcc ctggcaagta
      361 ccactacgcg gtctgcttct ggaacggcga gcggagcggc ggaatgaggg tggtcgatgg
      421 ccaggtgaag tccacgccgg cggatgacga ccgccgcgac gatcgctcgg aagacggtcg
      481 ctagaaccga acggcaacac cagcaccgac gacaccagtc ttcatcgacc ggtgagggga
      541 cccgaaaggg tcaggagagg cccgcgccgg gcgatgtcgg aggcggggcg gccccacaag
      601 ggtcgccccg ccggcttgtt gttatcgata caggtccacg accgtcgcga ccggcatcgc
      661 gaccgcagtt tcgaccctca cgtcaccgcg ccagattcgc tatcatcgcc ttcatgaagt
      721 ccggcaggtc cgccggcgtg cggctcgaga ccaggtggcc gtcgaccacc acgctccgat
      781 cgacgaaggt cgcgccggca ttctcgaggt cgtccttgat ggccgagacg ccggtgacgg
      841 tcttcccctt caggatgcgg gcgctgatcg gaacccagcc cgcgtgacag atcatcccga
      901 tcgtcttgcc ggcccggtcg aattcctgaa cgagttcgag gacacgagga tagcggcgta
      961 accggtcggg ggcccagcca cccgggatga ccaggccggc gaacgcgtcg gcgtcgatct
     1021 cggcgatggc ggcgtcggcg gggcaggggt agccgttctt gccgggatag gtcttccttt
     1081 cggggccggc caccaccacc tggtagcctt cctcctcgag tcggatccgg ggataccaga
     1141 gctcgagatc ctcgtaatag tgctcgacga agacgagaat cttcttcccc ttgccggggg
     1201 acgtatcggg ggacgtatcg ggggtcatcg ggacctcacg ggtgagggtt cgggacgaat
     1261 ggggctcgag gcgaatgggg ttcggggccc acggccgggg cacgttcgga cgggccgccc
     1321 tttatgctag tctcccccat tccccgatga acaaccaacc acgaattcga ccgatccggg
     1381 ctgccctgtc ggccctgccg atcctggcac tgctcctcta tggcctcctc gtgagttcgc
     1441 cggacggacg ggccgcgcgc ggcagcggtg ccaccgtcac ggccgacccc cgcgtcgagc
     1501 tcgcggcgct tctggcgcga tcggcgggcg acttcggcgc cccgtggccc accgaccttc
     1561 cgaccggctc cgtcgctcaa ctcgaccgga ccgtcgaatc gaagttcacg gcactttcca
     1621 cgctgccggc tccccggcgc gtcggcatgt acaggagcgc ggggcttacc gcggaagtcc
     1681 tcaccacgat tttcctgaat cgcggcccct ggccggagat gaagcgggaa gcgggtgaac
     1741 cgtacgacac cgaagccatc acacggctgc cgggcggacc caccttcctc gcgcggcgcg
     1801 agatcttcac gccgggtttc atcgactcgc tggtcgagga gacgagggaa ttcgctagcg
     1861 ccgccggctt cgaaaacgtg tggagctcca gccagcccat cctgctcgag cgcgccgaat
     1921 cgatcgagaa tgaccccacg ctgggccatc tcaccgcccg gctggccgac ttcctgggcg
     1981 agccctcgaa ggcggagccc tggatcgtcc cgacgcgctt cggcacctgg cgcacaccgt
     2041 tcggcgtccc ggtgaacgac ggggaccgca tcgtggtggt cgatcgcgcc gagagcgccg
     2101 gcaagctcac cccggaaacg agcctctcct ggatctgcgt ccgcgaattc tcccggccga
     2161 ccgtcgagcg cctggcgcgc gccaacaagg accggatcac cggcctggcc ggctactgga
     2221 cctacct
//
LOCUS       DASVOD010000247         7563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_278040, whole genome shotgun sequence.
ACCESSION   DASVOD010000247 DASVOD010000000
VERSION     DASVOD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278040"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VF720_05270"
     CDS             <1..393
                     /locus_tag="VF720_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7878796.1"
                     /translation="TWIAGHTDRFACYVNHAGVYDIHAQYASDVSQGRALAYGGEPWD
                     GLETIDMSSPARFAKGFNDPMLVLHGDNDYRVPATQGLEVYGVLKAKGVPARLVSYPD
                     ENHWILSPRNSRHWYGEVMGWLARYLKS"
     gene            404..2035
                     /locus_tag="VF720_05275"
     CDS             404..2035
                     /locus_tag="VF720_05275"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7878797.1"
                     /translation="MPMSPDHARLTELAEAYYRRGWERFPTSGSSAGLAEFDHRLEAP
                     DAGVMEDALADARDTLAKLEAIPAPAVGASIDEKLDRKEFEAQLRLAILHSGTIRNWR
                     RNPADPVDQVVSSLFFLLVRRDVDSPETAESMIGRLSAVPAFLDAVRSRTDDPVALWV
                     DVALESAEGGAPFLKELADGMKERHPSLAPKFDAAVARATPAMESYGKWLAGLKGKTL
                     REDPSIGREALTEMIRWNHGLSDSVDEVKAYGRKQMAELKARQVEVARAIDPAATPAE
                     LMARENRRWAETKPDMLAEYNRITFALRDRLVADGILELPPGEACDVIATPSFLRPMI
                     PTAAYSSPGPFDKRQRGLFYVTAPDPALDEADFIANVAQHFPLDPTCAHEAYPGHHVQ
                     LCWANQAPTLIRKLADHIIFMEGWTLYCEQLMVELGWYPSKVYELGYLNDQLWRACRI
                     VIDASVQSGEMTIDEAKAMLQQEVGFTPQRAQAELNWYTQSPGTPMSYLLGKGKTLAL
                     REAWMSKNQGGTLKQFHKWMLTQGSVPQGWLMEAM"
     gene            2038..3060
                     /locus_tag="VF720_05280"
     CDS             2038..3060
                     /locus_tag="VF720_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7878798.1"
                     /translation="MTPLTPDRIIQTALGFWPSKVVLSAMELELFTVLGRKPMTGEDL
                     RDALNLNERANPDFFDTLVALKFLERDGDGPTAFYRNSEETSAFLDKGNDGYIGGFME
                     MANARLYPFWGGLTEALRTGQPQNEIKHNGRPMFAELYSDPKRLELFMDAMSGISAPN
                     FAAFAEKFDFTRFQTMTDIGGATGQMSMFVARKHPHMKCKTLDLPEVTTIAKAKIAAA
                     ALGDQVSAAPIDFFTDSFPKADVITMGMILHDWNLEKKKMLIKKAYDALPVGGAFVVV
                     ENLIDDARRENAFGLMMSLNMLIEFGDAFDFTGADFRGWCKEAGFREFETIPLRGPAS
                     AGVAYK"
     gene            3223..5595
                     /locus_tag="VF720_05285"
     CDS             3223..5595
                     /locus_tag="VF720_05285"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7878799.1"
                     /translation="MPHRNGSRSGEAGFAVRTLVFFLLSATCMIPGKAAAVPFDSYPK
                     ILLHLRPVAQKNTCALGQLSDCRTALTSGGLSGPEGPFYFAYVLTARGSMPHVAGVQM
                     GISYQNDAPLGQLDGQGVDIFGWTNCATLEFTSRGWPASGGGNLITWDTTTRCQTGDT
                     GVAGYFYLGAYGPDRLSLIPRPVDHQAKLANCYSMETNLIPADLGWIDFSSGNLEAGC
                     NPCNALCGGPNGPDIYPPGAVTLNLWSRTTTTIRVQWYEPDEDGANGIGRPCVEYDLR
                     YSSSPLTEANFLSATQVLSMGTPSTPGNWRSRTVEGLLPSTTYHFALRSRDDAGLWSP
                     ISNSLEASTLFADDTTPPAAVTDLDVDDIDTTSVLLRWTAPGDDNSVGQATAYDLRYA
                     LTPITSASFGAASIAGGLPVPLPAGSSQTYLLTGLAENTRYYVAMRSADDRTNFSSLS
                     NVVTAVTLSSVPPDTTPPVPDVTPPAAITDLSVTNLYSNTVTLEWTVPGDDSLAGRAA
                     VYDLRRSTSPITSANFLSAPAVSGEPAPGLPGARQGMTLGGLAQSTRYYFAIRTGDEV
                     PNWSTPSNTADALTRGGLGDPRLMLHVASPISKSACANGQLARCNDAVTKGDLATVGG
                     GGPFYFVYLMGIEFGELAGLQCGIDYDQNRADGQGNHEAVDILGWTLCATMEFPSGAP
                     RQWPRPGSGNLITWDVSNRCQTGATAVAGYFYMVAYSADILKITPRPADAVAKASSCA
                     AVEKVIIPQFLGTARFSEGATLSGYNPCGAGQSIPVQHTTWSRIKTLARS"
     gene            5630..5971
                     /locus_tag="VF720_05290"
     CDS             5630..5971
                     /locus_tag="VF720_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015831628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEX7878800.1"
                     /translation="MPPIVKTIVLLILSNVFMTFAWYAHLKNLSGKPWIMAAFASWGI
                     ALFEYLLQVPANRIGYTQLQLGQLKILQEVITLAVFVPFAVFYMKEPVRLNYLWAGLC
                     LVGAVYFVFRK"
     gene            6098..>7563
                     /locus_tag="VF720_05295"
     CDS             6098..>7563
                     /locus_tag="VF720_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878801.1"
                     /translation="MPPRRSWLAIIALSALLLSAPLSAEAAPFDNRPKILLHLTNPTT
                     KNACDAGRLSDCTTATTAGDLAIEGSGPFYFAYVLTAKGNLTDVAGLQFGLNYQDGLV
                     GNSSDGVGLDIFQWVQCATYEFAASFRPWPEPGGSNLIVWNALTVCQTGETGVAGYFY
                     MGAYGPDALRLTPRPIDGLAKLANCLSIETILEPTDLGIVAFGSGSEHSGCNACLESC
                     GPPIPPPDTVPPDRIVLSLGTTTSSTATLQWPAPDEDGPTGYGHWCRSYDMRQSTVPI
                     TETNFTSLNEVTNLPNPTNVGFLQSKTIFGLVPGETNYFAIRAIDDGQNAGPISNVIA
                     VTTSVGPDVFPPAAITDLAIAHVGGTELYLEWTAPGDDEDSGQASRYDIRYSLDPITA
                     SNFGAASQSDNEPVPGLAGTPESFVLSGLVSGIQYYVAMKSLDEVPNSSALSNVVTER
                     TIRPTTLDTTPPAAITDLIVTGSDLNQVHLEWTATGDD"
BASE COUNT         1354 a         2623 c         2283 g         1303 t
ORIGIN      
        1 acctggatcg ccggccacac cgaccgcttt gcctgctacg tgaaccatgc cggcgtgtac
       61 gacatccacg cccagtacgc cagcgacgtg agccagggac gcgccctggc ctacggtggc
      121 gagccgtggg acggcctcga gacgatcgac atgtcgagcc ccgcgcgatt tgcgaagggt
      181 ttcaacgacc cgatgctggt gctccacggc gacaacgact accgcgtgcc ggccacgcag
      241 gggctcgagg tgtacggcgt gctcaaggcc aagggcgtcc cggcgcgcct cgtgtcgtac
      301 ccggacgaaa accactggat actctctccc cgcaactcgc gccactggta cggcgaggtg
      361 atggggtggc tggcccgcta cctgaaatcc tgacggagac gacatgccga tgtcaccaga
      421 ccacgcccgg ttgaccgaac tcgccgaggc ctactaccgc cgcggctggg agcgctttcc
      481 cacgtcggga tcgagcgcgg ggctcgccga gttcgaccat cggctggaag cgcccgacgc
      541 cggcgtcatg gaagacgcgc tggccgacgc gcgcgacacg ctggcgaagc tcgaggccat
      601 cccggcacct gccgtcggcg cctcgatcga cgagaagctt gaccgcaagg agttcgaggc
      661 ccagctccgg ctggccatcc ttcattccgg cacgatccgc aactggcggc gcaacccggc
      721 cgacccggtg gaccaggtgg tgtcgagcct cttcttcctc cttgtgcggc gcgatgtcga
      781 ctcccccgaa acggccgagt cgatgatcgg tcgcctgtcg gccgttcccg ccttcctcga
      841 cgccgtgcgt tctcggaccg acgacccggt ggccctttgg gtggatgtgg ccctcgaatc
      901 ggccgaaggg ggcgcgccgt tcctcaagga actggccgac ggcatgaagg agcgccatcc
      961 cagtctggcc ccgaagttcg atgccgcggt ggccagggcc acgccggcca tggagtcgta
     1021 cggaaagtgg ctggccggcc tcaaggggaa gacgctgcgc gaagacccga gcatcggccg
     1081 cgaggcgctc acggagatga tccgctggaa tcacggtctt tccgattcgg tggacgaggt
     1141 caaggcgtat ggccgtaagc agatggccga gctcaaggcc cggcaggtgg aggtggccag
     1201 ggccatcgac cccgcggcca ctcccgccga gctcatggcc cgcgagaacc gccgctgggc
     1261 ggaaacgaag ccggacatgc tggcggaata caaccggatc acgtttgccc tgcgcgatcg
     1321 gctggtggcc gacggaatcc tcgagctccc tcccggcgag gcgtgcgacg tcatcgccac
     1381 cccgtcgttc ctccgcccga tgattcccac cgccgcgtat tcctctcccg gccctttcga
     1441 caagcgccag cgcggcctct tctatgtgac cgctccggat cccgcgctcg acgaggccga
     1501 cttcatcgcc aacgtggccc agcacttccc gctcgacccc acctgcgccc acgaggccta
     1561 ccccggccac cacgtgcagc tctgctgggc caaccaggcg ccgaccctga tccgcaaact
     1621 ggccgaccac atcatcttca tggaaggctg gaccctgtac tgcgaacagt tgatggtcga
     1681 gctcggctgg taccccagca aggtgtacga gctcggctac ctgaacgacc agctctggcg
     1741 ggcctgccgc atcgtcatcg acgcctccgt ccagtcgggc gagatgacga tcgacgaggc
     1801 caaggccatg ctccagcagg aagtgggctt caccccgcag cgcgcccagg ccgagctcaa
     1861 ctggtacacc cagagccccg gcacgccgat gagctatctc ctcggcaagg gaaagacgct
     1921 ggcgctgcgc gaggcctgga tgtcgaagaa ccagggcgga acgctcaagc aattccacaa
     1981 gtggatgctc acccagggct cggtgccgca agggtggctg atggaggcga tgtaaccatg
     2041 accccgctca cccccgaccg catcatccag accgccctcg gtttctggcc ctcgaaggtc
     2101 gtcctctccg cgatggaact cgagctgttc acggtgctcg gccggaagcc catgaccggc
     2161 gaggatctcc gcgacgcgct caacctgaac gagcgcgcca accccgactt cttcgacacg
     2221 ctggtggcgc tcaaattcct cgagcgggac ggcgacggac cgacggcttt ctaccgtaat
     2281 tccgaggaga cctccgcctt cctcgacaaa ggcaacgacg gttacatcgg cggcttcatg
     2341 gagatggcca acgcgcgcct ttacccattc tggggcggcc tcacggaggc gctcaggacc
     2401 ggccagccgc agaacgagat caagcacaac ggccgtccca tgttcgccga actctacagc
     2461 gacccgaaga ggctcgaact gttcatggac gccatgtccg gcatctcggc gcccaacttc
     2521 gccgcgttcg ccgagaagtt cgacttcacc cgcttccaga caatgaccga catcggcggc
     2581 gccaccggtc agatgtcgat gttcgtggca cgcaagcacc cccacatgaa gtgcaagacc
     2641 ctggacctgc ccgaagtgac gaccatcgcc aaggctaaga tcgccgccgc cgctctcggc
     2701 gaccaggtga gcgccgcccc catagacttc ttcaccgatt cgttccccaa ggccgacgtc
     2761 atcaccatgg ggatgatcct ccacgactgg aatctcgaga agaagaagat gctcatcaag
     2821 aaagcctacg acgcgcttcc ggtcggcggg gccttcgtcg tcgtcgagaa cctgatcgat
     2881 gacgcccggc gcgaaaacgc cttcgggctg atgatgtcgc tcaacatgct gatcgagttc
     2941 ggcgatgcgt tcgactttac cggtgccgac ttccgcggct ggtgcaagga ggcggggttc
     3001 cgcgagttcg agacgattcc gctgcgcggg ccggccagtg cgggggtggc gtacaagtag
     3061 ccgatgcccg aaaagggggc gatcggggga aatacggggc cgggttgcgc attttggcgt
     3121 ggtccggccc cactttcttc cctagactgg gaacgggtcg gtcctgtcgg accctttcct
     3181 ccttctcctc ctatccatcg acgacagcct tggagaccgg aaatgccgca ccggaacggg
     3241 agtcgatccg gcgaagctgg cttcgccgtc cgcacgcttg ttttcttcct gctcagcgcc
     3301 acctgcatga ttcccggcaa ggccgccgcc gtcccgttcg acagttatcc caagatcctc
     3361 ctgcacctgc gcccggtcgc gcagaagaac acctgcgctc tcggacaact ttccgattgc
     3421 cgcaccgctc ttacctccgg agggttgtcc ggcccggaag ggcccttcta tttcgcctat
     3481 gtcctgactg cccggggttc gatgccgcac gttgccgggg tgcaaatggg catcagctac
     3541 cagaacgacg cgccgctcgg gcagttggat ggccagggtg tggacatctt cggctggacc
     3601 aactgcgcca cgctggaatt cacgtcgagg ggctggccgg cgtccggcgg cgggaacctg
     3661 atcacctggg atacgacgac gcgctgccag accggcgata cgggggtggc gggctacttc
     3721 tatcttggcg cctacggacc ggacaggcta agtctgattc ctcgccccgt cgaccaccag
     3781 gccaagctcg ccaattgcta ttcaatggaa accaacctga ttcccgccga cctcggctgg
     3841 atcgacttct cgtcgggcaa cctcgaagcc ggctgcaacc catgcaatgc gctgtgtgga
     3901 gggcccaatg gaccggacat ctacccgccg ggcgccgtca cgctcaacct ctggtcgcgg
     3961 acgaccacca ccatccgggt ccagtggtac gagcccgacg aggatggtgc gaacggcatc
     4021 ggccgcccgt gcgtggaata cgaccttcga tactcgagct cgcccctgac cgaggccaac
     4081 ttcctctcgg ccacgcaggt cctgtcgatg ggaacgccgt cgactcccgg caactggcgc
     4141 agcaggactg tcgagggcct gctgccgagc acgacctatc acttcgccct ccgctcccgc
     4201 gacgatgccg ggctgtggag ccccatctcg aacagcctgg aagcctccac cctgtttgcg
     4261 gatgacacga cgcccccggc ggccgtcacc gaccttgacg tggacgacat cgacacgacc
     4321 agcgtgctcc tgcgctggac ggctccgggc gacgacaact ccgtcggtca ggcgacggcg
     4381 tacgacctgc gctacgcgct cactcccatc acgtcggcct ccttcggtgc cgcgagcatc
     4441 gcgggcggcc ttccggttcc cctgcctgcc gggtcgtccc agacttacct gctgaccgga
     4501 ctggccgaga atacgcgcta ctacgtggcg atgcgctcgg cggatgaccg gacgaatttc
     4561 tcgagcctgt ccaacgtggt gacggccgtc accctttcga gcgttcctcc cgataccacg
     4621 ccgcccgtgc ctgacgtgac cccgccggcc gccatcacgg acctgagcgt caccaacctc
     4681 tacagcaaca ccgtaacgct cgagtggaca gtgcccggtg atgacagcct ggccggacgg
     4741 gccgcggtgt acgacctgcg gcgctcgacc tccccgataa cgtccgccaa tttcctttcg
     4801 gctccggccg tcagcggtga gcccgccccc ggcctgcccg gggcgcgcca gggaatgacc
     4861 ctgggtggcc tggcgcagag cacccgctat tacttcgcca ttcgcaccgg ggacgaagtg
     4921 ccgaactggt cgactccctc gaacacggcc gacgccctga ctcgcggcgg actcggcgat
     4981 ccgcgactca tgcttcacgt ggcgtctccc atcagcaagt cggcctgcgc gaacggacag
     5041 ctcgcgcgct gcaacgatgc cgtgacgaag ggagacctgg ccaccgtggg cggcggaggt
     5101 cccttctact tcgtctacct gatggggatc gagttcggtg agctggccgg cctgcagtgc
     5161 gggatcgact atgaccagaa ccgggccgac ggacagggca accacgaggc agtggacatc
     5221 ctcggatgga cgctctgcgc cacgatggaa ttcccgtccg gcgcgccgcg ccagtggcca
     5281 cgccccggaa gcggcaatct catcacgtgg gacgtgtcca accggtgcca gaccggcgcg
     5341 acggccgtgg cgggctactt ctacatggtc gcctactcgg ccgacatcct gaagatcacg
     5401 ccgcgacccg ccgacgccgt ggccaaggca tccagctgcg ccgccgtcga gaaggtcatc
     5461 attccgcagt tccttggcac cgctcgattc agcgagggag ccaccctgtc cggatacaat
     5521 ccgtgcggtg ccggccagag catcccggtt cagcacacga cgtggagtcg gatcaagaca
     5581 ctcgcccgat cataggcgca tcaacgcccg cgtaccgtac aatgccccga tgcctcccat
     5641 cgtcaagacc atcgtcctcc tgatcctctc gaacgtcttc atgacgttcg cctggtacgc
     5701 ccacctcaag aacctgtccg gaaagccctg gatcatggcc gccttcgcca gctggggcat
     5761 cgccctgttc gaatacctgc tccaggtgcc ggccaaccgg atcggctaca cgcagctgca
     5821 gctcggccag ctcaagatcc tgcaggaagt gattacgctg gcggtgttcg tgccgtttgc
     5881 cgtcttctat atgaaggagc cggtgcggct gaactatctc tgggctgggc tgtgcctggt
     5941 tggggcggtt tattttgtgt ttcggaaata ggatgtcgaa ctgaaattat gggtggcctg
     6001 cggccagctt tgtatctaaa ctgatcaggc cgcccgggga cccgggcggc cttccaattg
     6061 gttacgcttc attgaattgc catcaaggag accggacatg ccgccccgga gatcctggct
     6121 tgccatcatt gccctgagtg ctctattgct cagcgccccg ctctcggccg aagccgcacc
     6181 cttcgataac cgcccgaaga tcctcctgca cctcacgaat ccaaccacga agaatgcctg
     6241 tgatgcggga cggctctcgg attgcacgac ggcaaccacc gctggcgatc tcgcaatcga
     6301 gggatcggga ccgttctact ttgcctacgt gctcacggcg aagggcaacc tcaccgacgt
     6361 ggccggcctg cagttcggcc tgaactatca ggacggcctc gtgggcaact cgagcgatgg
     6421 ggtggggctc gacatttttc aatgggtcca atgtgcgacc tatgagtttg ccgcctcctt
     6481 ccgcccgtgg cccgaacccg gggggagcaa cctgatcgtc tggaatgcgc taacggtctg
     6541 ccagaccggt gagacgggtg ttgccggcta cttctacatg ggcgcctacg gccctgatgc
     6601 gctgcgcctc acgcctcggc cgatcgacgg cctcgccaag ctggccaact gcctctcgat
     6661 cgaaactatc ctggagccta ctgacctcgg aatcgttgcc ttcggctccg gctcggaaca
     6721 ttccgggtgc aacgcgtgcc tcgaaagctg cggcccaccg atcccgccgc ctgatacggt
     6781 gccacctgac cggatcgtac tttcactcgg caccacgacg agctcgaccg caacgctgca
     6841 atggccagcc cccgatgagg atggcccgac ggggtacggt cactggtgcc gttcttacga
     6901 catgaggcaa tcgaccgtac caatcacgga aaccaatttc acttcgctca acgaggtgac
     6961 gaaccttccg aatccgacga acgtcgggtt cctccaatcg aaaaccatct tcggcctcgt
     7021 tcccggcgag accaactact ttgcgatccg cgcgatcgac gacggtcaga acgccggtcc
     7081 catttccaac gtgattgccg tcacgacttc tgtcggaccg gacgtcttcc cgccggccgc
     7141 tatcacggac ctggcgatcg cccacgtcgg cggaaccgag ctgtacctgg agtggacggc
     7201 cccgggggat gacgaggatt ccggccaggc aagccgttat gacatccgtt actcgctcga
     7261 tccgatcact gcctccaact tcggagccgc cagccagtcc gataacgagc cggtcccggg
     7321 acttgccggg acgccggagt ccttcgtgct ctccgggctg gtctcgggca tccagtacta
     7381 cgtggcgatg aaatccctgg acgaagtgcc caattcgtcg gccctgtcga atgtcgtcac
     7441 cgaaaggacc atccgaccga cgacgctgga tacgacgcca ccggcggcca tcaccgatct
     7501 catcgttacg gggtcagacc tgaaccaagt tcacctcgaa tggactgcga ccggcgatga
     7561 cag
//
LOCUS       DASVOD010000248        17420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_956167, whole genome shotgun sequence.
ACCESSION   DASVOD010000248 DASVOD010000000
VERSION     DASVOD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 17420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17420
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956167"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2005)
                     /gene="thrA"
                     /locus_tag="VF720_05300"
     CDS             complement(<1..2005)
                     /gene="thrA"
                     /locus_tag="VF720_05300"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HEX7878802.1"
                     /translation="MIVMKFGGSSLATPRRIQEVAALVRAARKTGRVVTVVSAFQGVT
                     NELLRLARLAESGDDSWRAPFRKLAARHRSALSAVAAGPRARKAGERLGAQLDELRDA
                     LTGIRLLRDAHPRSLDVVASFGERMSALVVSAALGPAGVDVDTRPLIVTDERFTDAQV
                     NFDATRPRLSARFRRLFAASPKALPIVTGFIGATPDGRTTTIGRNGSDYTAAIVAEAL
                     GARAIEIWTDVDGILSADPAVVPGAFVVPRLSYEEAMELSYFGAKVIHPAAIAPAVRA
                     GIPLVVKNTMNPEAAGTVVSATPDPAEAVAKGITAIDGMTLLTLRGAGLVGVPGVAQR
                     LFGALAARQVSVILISQASSEHTICFAVESGDAARARRGIAGEFQREMADGLTALEES
                     PGRTIVAIVGEGMRGTPGVSGRVFDALGRHGVSIGAIAQGASERNISFVIDGAQASLV
                     VNVLHEAFFETTRKLAVAVLGAGNIGAALLDQLRGQRASLLARGFDIRVVAVADSRKL
                     LFDPAGVNLARWRADLAATRTVMSPAELLRRLGTVRMPDRAIIDCTASAPLVDAYADF
                     LNADCHLVTPNKKANVLPPAEYDALLALVRSRRKQFLFEANVGAGLPVISTLHDLVAS
                     GDRVDRIEGILSGTLSSLFNRLTDATTFSELVRQARDEGLTEPD"
     gene            complement(2080..4434)
                     /locus_tag="VF720_05305"
     CDS             complement(2080..4434)
                     /locus_tag="VF720_05305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX7878803.1"
                     /translation="MTPPLPGSREVPPGTDPAVVAQYERWFKRLDGQLRVLDRERQKF
                     LAVVNRTDAEVFVADVDRTIRWTNRHLVDCEAGGDPNGWHGRKCSDFCSHLAGASCGP
                     GALDCPVARALRDNEAAHREIHHRTGDVVRELYLSALPILGPDGRPEEAVVLVQDLSD
                     LEIVRRLEARYRTLFDRSGNAILMLDPGTLRIVLANDTACRLLGHTTDELLAMTLDQL
                     HSPGEWARLERIYRDAFAGTTTPTFEAIAWTGDSEERVVEVGLSPLELDDQNLVVVEF
                     RDLTERRRAETALRHSQDQLRTVVSNAPIVLFTLDGNGRFTMSEGRGLEKLGLKPGEV
                     VGKSVFDVYADHPAICDANRRALAGHEFKEIVDVGPLAYETWFSPLRNSRDESEGVLG
                     VAIDVSASRRLEQQLRHSQKMEAIGRLAGGVAHDFNNLLTVILGHCEMMTRKVEPGSA
                     LYREAEAVLKAGRRGADLARQLLAFSRKQLVEPTIIDLNRVLGDLDAMLRRLIPEDIA
                     LVTVVGTAPSQIRADAGQVEQIVMNLVVNARDAIESGGKITVEVARVTVDETFAAAHP
                     SLPPGAYVQLSVSDNGRGMDADTRLKIFEPFFTTKEAGRGTGLGLSIAYGIVRQYGGD
                     IQVYSEIGQGTTFKVYMPEVVLEPTTLAAPPPGLRGETGSGTILLTEDEEEVRALARE
                     ILEMCGYTVLEAACGEEAIALSEAHPGPIHLLLTDVVMPGLSGGEVAQRMQRLRPGIK
                     VVFMSGYPDDAIVHHGVLDAEQAFVQKPFSIEELSGRIREMLVA"
     gene            complement(4431..4916)
                     /locus_tag="VF720_05310"
     CDS             complement(4431..4916)
                     /locus_tag="VF720_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7878804.1"
                     /translation="MTLSDLLLEIDRMDAEGLDLEDILEATMQKLSTTNPLFHWTGIY
                     ELFPDDVLRLGPFVGAPTDHVFIGVGRGVCGRAVAERRNMNIPDVRAESNYLACSTQT
                     RSELVVLIRMGDQIYAQIDIDSHQLAAFDDAAVALVQGVADRLALLYERRSRGTEAGA
                     A"
     gene            5004..5876
                     /locus_tag="VF720_05315"
     CDS             5004..5876
                     /locus_tag="VF720_05315"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7878805.1"
                     /translation="MIGLVLNPEEGPGRPVASVLWIEDHELEEWVRNGRVTPDTWVWA
                     GPWSGGRWKRADDLEVYHLFRPESVEVRPPGISLRDTVFPTRGLSATESLMLLNIAVS
                     AILLAVWREAYETELWQTMAWWWTRVTTDLEWWRWLPTLVMHADAGHLLRNLGALLVT
                     AGGVEVFFGRLRTWLFYVATGVAGAVASFLGRAEAPLSVGASGAIFGLAGVLTGFLFR
                     HAGRFSERQRWKARRVYAPLILVLVPPSLFHADWRAHLGGYVLGVILGLVTPLGAAGR
                     RVLAGKEISSGSGS"
     gene            complement(5854..6603)
                     /locus_tag="VF720_05320"
     CDS             complement(5854..6603)
                     /locus_tag="VF720_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878806.1"
                     /translation="MRLRNRALSEGIPTTLLLGLLLASPASAVPQPYTFEGLAAGTIV
                     AGVKPNGQILASDPYPGFEFSVIGNAASNSLCIFDSAHPTGADLDLGTPNDGFGGPGK
                     GNGGKPGMPGQNKTPQGKVLVIPENLVDVNQDGRVDTPDDNHLGGQITVNWTVQGLLL
                     KVVLLDIEESGAFVDCYKGNQLVQSFPVPALGDNSQVEVLTETTTAIDRTVITLPGSG
                     SLAAMTFDNGPVIPVPVEDATWSRIKNLTRN"
     gene            6707..7276
                     /locus_tag="VF720_05325"
     CDS             6707..7276
                     /locus_tag="VF720_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX7878807.1"
                     /translation="MQISTLLAGRRSTRSFAQREVVQEDIDALFEAARWAPSSGNRQP
                     WRYLFRTSRAGHPGMSACLTRGNQWALAAPLLVVQLTRVADGGELNGVPYAYVGCGLS
                     LMSLIVEAESRGLRAHPMAGWIDQPLRAEFDVPADWQPVVVVAVGYEGTLDQLDPALR
                     EKELRPRTRRPLAEVMVEGRWPSGWSGPL"
     gene            complement(7253..8272)
                     /locus_tag="VF720_05330"
     CDS             complement(7253..8272)
                     /locus_tag="VF720_05330"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX7878808.1"
                     /translation="MADDPRTPAAEAPPPEPEWGGPGPVKVLALLNAGAGKGDVEKIR
                     EGIQKGLEETRAVIDFIEVGGTVHARDKTRTALDAGYQLVLVAGGDGTLADAAHGLVG
                     SPVPLGVIPAGTGNIVAINLGIPMAPRDAARAAWFGTALPYDVGRLDDGRIFVLSAGA
                     GYDADLIRDADRELKRRFGPLAYLIAMFKNLRVKRARYIIEMDGREKVHVLAKSVLVT
                     NVGRTMGALPLAPDARVDDGRLDVVVFTFATLPELVILFVRALLGGLKQDPRVRFYQA
                     ERIRVVASRPIPVQIDGELIERTTPIEIDLLPGALRIMRPATRPTLDLAALWPERPSK
                     RTRPA"
     gene            complement(8299..9528)
                     /locus_tag="VF720_05335"
     CDS             complement(8299..9528)
                     /locus_tag="VF720_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878809.1"
                     /translation="MLIKRLRPVPGLLTLAALGFAIPGTSVAGPTDPFATSDLTPLAT
                     TDTDPRQAAIDHLAIQTRDGLTMLAHWYIQGSMTPLPEPRSAEFMYTLGAHRGEKAWS
                     DAALGATGAWVGLHLNDYLKWGVADSTRTDVPANGREARKVKTLTGQSELLELFTMAF
                     QASGKELFRKAARSTAGDLLAWSRTADGVLAWTSNEPDTLAGPMASDAEMRLADERLA
                     RAALVFGEPAWGVGGGNPAPTPLPAPAPPVTPVTQADSIATARSALDLIRQAMWTHAD
                     SLIEEAGRRLEPLQLATAPETRVMPLFAARASAGLALELSVRPSVMAYLVGDSTSEGI
                     RQLRDAAVRAWRPGKLVQVRSAGAEDLLYPASEDGKPLAYVCSGELCAPPTSDPTEVL
                     ALVGSFALPEAPGAPGQ"
     gene            9667..10362
                     /locus_tag="VF720_05340"
     CDS             9667..10362
                     /locus_tag="VF720_05340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7878810.1"
                     /translation="MTGPSNDRLPRRVPVILVRHGLTEWNDLGLIQGWTDIPLSPLGR
                     RQAEATARALAGRPISAVASSDLVRARETAEAIARAHGLTPTLHADLREYHCGEWEGR
                     PFLEVRRDHPDAFRAWFNDAEAPIPGGESMGAALRRAGPAVDALLASLAGEGALVLVG
                     HGGINRVLAAHFLAMPLAHARRFMLDNASISIFEPHHSGWALRSWNHTGHLDALADET
                     GGDARRTDAPRTN"
     gene            10373..11614
                     /locus_tag="VF720_05345"
     CDS             10373..11614
                     /locus_tag="VF720_05345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7878811.1"
                     /translation="MWRQFDRLLEGRVFPALLLLGVVLRVAAIVSFPVYPLVDNTADT
                     AIYDEGARSLAAGEGYRWGGRLTAFFPIGWPLLLSMAYRVAGESARSGQALNFLFSLV
                     LLLACWFLVHRLAGRRAGRLAALVLAFAPHHVVYPAFLMSETSFTAFFLLSLGLVAVA
                     VPPGREHGEKLPLLIGAGVLMGAATLIRGPALVFPLAVAAWTFVSERGRAVPRMLRSM
                     LCAFVFGLGLLLAVAPWATRNHRVFDRWVLVANDGGMNFLMGNHHGATGARHEPVEGL
                     PDLGNEVADDREGYRRGAAFIKENPGEFLTLLPKKFVRLVAPASLLTYRAELRAKWPE
                     PLALTLLAIDQLLHAVLWIMALVAVLKLRSHPAVRFGAVMIGLWVLIHLAFLGGARYF
                     YPVMPLLLLVAASARFSEREA"
     gene            complement(11595..12881)
                     /locus_tag="VF720_05350"
     CDS             complement(11595..12881)
                     /locus_tag="VF720_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7878812.1"
                     /translation="MTPASAIPLPCCRRARGPCALAVVLALAAMLTGSPPARARTWHL
                     TPGGGDAVSIQAAIDSAADGDTLLLATGIYSERSRIVNKTLYMFGAGVERTTLDGGAQ
                     VGEHGHTLQIRGTGNYSEVRDLTIRDGNANSPQDIDGGEYGGGVLGVEANFAIIGCRF
                     LDNSCSSLGGGLFATSLPLIQLSHAGGGSRSTGPRRAQVAGPRDEILVQDCLFENNFG
                     GSEGGGVCFDQAYFRVVNCTFRHNFSGQGGGAAIINAVGSVSLSLFVDNEAILDGGGL
                     QIMTGLALPPPTIRIFQNTFVKNRCDRHGSGLALYDGTTIEVNANVFDGQTGGAFSVL
                     GCANGAIYAGGCNLFGTNASPPLEGCPPIETDVFGPPMFCNAEGGDYRVCTNSPAAVS
                     SGNCPVVKGIGVGCTGVACATVVRPATWSGIKLLVR"
     gene            12862..13719
                     /locus_tag="VF720_05355"
     CDS             12862..13719
                     /locus_tag="VF720_05355"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7878813.1"
                     /translation="MAEAGVIETILSSARGSRPAARVVFRSWQPTDPVAAVILLHGLG
                     GHSDRFQECGSFWASRALAVYAPDLPGFGHTDGPKGHLDSFEPMFTTIEVILARVMAD
                     HSRLPVFIVAESMGAAVAIDWVSRHPALLSGLVLLAPALKDRLNVPVWRKVEALVHVI
                     VRRRKYYDVPFEPDNFTRDAAIKEVLAADPLEVRRVTAQFYFAWLPVTARALKAAPLL
                     KLPVAVLLPGKDTMIDSDATRVWFEGLPEATRTLVEYPGLLHGLLVELDRQPVLDDIA
                     AWIRKQVGR"
     gene            13769..14545
                     /gene="lgt"
                     /locus_tag="VF720_05360"
     CDS             13769..14545
                     /gene="lgt"
                     /locus_tag="VF720_05360"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEX7878814.1"
                     /translation="MLPILFEIGPFKLHSFGLMMALAFLAATLIAARRFRRLGLDPDR
                     ASELGLGAMVGGVLGAKVYYLIDHWSDTAADPMKMIFSGSGLTWYGGLLGGVIGTLAV
                     AKWRKVPLLWLCDLAAPSLGLSYAIGRMGCFLNGDDYGRPTDVPWAMAFPKGTPPTTT
                     PVHPTQLYETAGGLLIFWVLSRLGDDRLGTGLIFGLYLLMAGFERFVVEFYRTNEPLA
                     AGLTLAQWISVGLMLAGVVFVSRARAGQRKGLATAATAGG"
     gene            complement(14484..15653)
                     /locus_tag="VF720_05365"
     CDS             complement(14484..15653)
                     /locus_tag="VF720_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7878815.1"
                     /translation="MSPRDDNTTLRRFRPLRLALLLLAITAGFGGCGGDDDPVGPTPE
                     GYRTWHTTPDGTGSDCGMPQECLDAAAAGDTIEFAAGGYDTPADTLVDDGMGGVVSAI
                     LSARRSQVLRAAPGADVRVNGMWRPGSLGLHVRAGDDTVVVRGIRFDNCDAGLRAAGG
                     TVMIEECEFVSGQHGIVADDAELDIRNSLFMEYAAEAMVLRNCTGRMTRSQFWGNTYG
                     PYLAESRDFTLEHTLIALCCLTGIRIEEGGVVRLRNVTVTGVGMVPDDSTGVVVAGGA
                     HAVIERSVIAHNRGFGIDCRTGGSAEVRCSDVVQHSSGNYHGCPDVTGTMGNLSVDPG
                     FCGPDDLDFHFKADSPARLASCGGMGAFVDEPCGPFNRPPWRRWPDLSVARPGRD"
     gene            15698..17050
                     /locus_tag="VF720_05370"
     CDS             15698..17050
                     /locus_tag="VF720_05370"
                     /inference="COORDINATES: protein motif:HMM:NF040439.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX7878816.1"
                     /translation="MVPSVTSPAHCNPARRTVFVRVLVALFLAVAFAPNARAQDRSPA
                     PGPPGVSRGGLNYAAELSVRRNAAGATVLDVLVEVPYSSLRFARDAGGWVARFDVTVL
                     VYDRSGNQVNGDLWTIPVRSTDAARDQSRGLSIRRRYPLIVTEGRLKVDISAVQSGSG
                     REGTWTRTIEVPRWDGTEMALSLPMFGRCDLSTATRDSSWSPPDTSWGPAFAPVVRRR
                     YGDSQPDLCVRGEVYDQDPSDAPAYRFDWSIEGEHDRKGGSGSFEVPRVNHRGTWMVH
                     PPIDSLSRGEWRLFVTARLANRSVRIEERFEIDESRLNVLDDADMIRGVLAYIADNEE
                     LVRLENLPVDSLATFWDQFWRRRDPSKDTPRNENRDEFMRRVEYVNAGYSIMDAGWRS
                     DMGRIYIRYGAPDQVEKVPYTSEGPPHEIWYYTERNLRFVFVDSEGFGRFRLVGRERP
                     "
     gene            17047..>17420
                     /locus_tag="VF720_05375"
     CDS             17047..>17420
                     /locus_tag="VF720_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX7878817.1"
                     /translation="MTRLGKRLIEVSVAAIQKEPSHAAELVTQAVMGVRVRVLDEADG
                     GRWLRVVLPDGYRGWLRAWLAAPDVATWPGPRVAEVDVPWTWVLPAPGRHDEPVSDVV
                     IGTRLAVLGRADGGWQPVALPDG"
BASE COUNT         2571 a         5859 c         6094 g         2896 t
ORIGIN      
        1 ggtcgggctc cgtgagtcct tcatcgcgcg cctgccgcac cagttcactg aaggtggtgg
       61 cgtccgtcag gcggttgaac agcgaactga gcgttccgga aagaattccc tcgatgcggt
      121 ccacgcggtc gccggaggcc accaggtcgt gcagcgtgga aatcaccggc agccccgctc
      181 ccacgttggc ctcgaagagg aactgcttgc gccgcgagcg gaccagcgcc aggagcgcgt
      241 catactcggc ggggggaagg acgttggctt tcttgttcgg cgtcaccagg tggcagtcgg
      301 cgttcaggaa gtcggcgtac gcgtccacca ggggtgcgga agccgtgcag tcgatgatcg
      361 cccggtcggg catgcggacg gtgcccagcc ggcggagcag ttcggcggga ctcatcacgg
      421 ttcgagtggc ggccaggtcg gcgcgccagc gggcgaggtt cacgcccgcc gggtcgaaga
      481 ggagcttccg gctgtcggcc accgccacca cgcggatgtc gaagccgcgg gccaggaggc
      541 tggcacgctg gccgcgcaac tggtcgagca gcgccgcgcc gatgtttccg gcgccgagta
      601 ccgccacggc cagcttcctg gtggtctcga agaaggcctc gtgcagcacg ttcaccacga
      661 gcgaggcctg cgcgccatcg atcacgaagg agatgttccg ctccgaggct ccctgggcga
      721 tggcgccgat cgacacgccg tgacggccga gcgcgtcgaa cacgcgcccc gagacgccgg
      781 gcgtgccgcg catcccctcg cccacgatgg ccacgatcgt ccggccgggt gattcctcga
      841 gggcggtcag gccgtcagcc atctcgcgct ggaattcgcc ggcgattccc ctgcgggcac
      901 gcgccgcatc tcccgactcc accgcaaagc agatggtgtg ctcgctcgac gcctgcgaga
      961 tgaggatcac gctcacctgt ctcgcggcca gcgcgccgaa gagccgctgg gccacgccgg
     1021 gcacgcccac gaggcccgcg ccccggagcg tgaggagcgt catgccgtcg attgccgtga
     1081 ttcccttggc caccgcctcg gccgggtcgg gcgttgccga caccaccgtc ccggcggcct
     1141 cggggttcat cgtgttcttc accaccagcg gaatccccgc ccgcacggcc ggcgcgatgg
     1201 ccgccgggtg aatgaccttg gcgccgaagt acgaaagctc catggcctct tcgtacgaca
     1261 ggcgggggac gacgaatgcg ccgggcacta ccgccggatc ggccgacagg atgccgtcga
     1321 cgtcggtcca gatctcgatg gcgcgggcgc cgagcgcctc ggccacgatg gccgccgtgt
     1381 agtccgaccc gttccggccg atcgtggtgg tgcggccgtc gggcgtggcc ccgatgaacc
     1441 ccgtcacgat cggcagcgcc ttcggcgagg ccgcgaagag gcggcggaag cgggcggaca
     1501 atctcgggcg cgtggcgtcg aaattcacct gggcgtcggt gaatcgctcg tcggtgacga
     1561 tcagtgggcg cgtatcgaca tccactccgg ccggaccgag cgccgcgctc accaccaggg
     1621 cgctcatccg ctcaccgaaa ctggccacca cgtcgagcga gcggggatgg gcatcgcgca
     1681 gaaggcggat accggtcagg gcgtcacgca gctcgtcgag ctgggcaccc aggcgctctc
     1741 cggccttccg cgcgcggggt ccggcggcca cggcggacag tgccgatcga tgccgggccg
     1801 ccagcttccg gaacggggcg cgccacgagt catcgcccga ctcggccagt cgcgccagcc
     1861 ggaggagctc gttggtgacc ccctggaagg ccgacacgac cgtgacgacg cgaccggtct
     1921 tccgcgcggc gcgcacgagg gcggccactt cctggatgcg gcggggcgtg gccagcgagc
     1981 tgccgccgaa cttcatcacg atcatgaggc cgcggattct agccgtggcg gccggcagcg
     2041 tcgagggacg cccgcagttc gatggccgga cccgaggcct caggccacca gcatctcccg
     2101 aatgcgtccg gagagttcct cgatggagaa gggcttctgc acgaaggcct gttcggcgtc
     2161 gagtacgccg tggtgcacga tggcatcgtc cgggtagccg gacatgaaca ccaccttgat
     2221 ccccgggcgg agacgctgca tccgctgggc cacctcgccc cccgacaatc cgggcatgac
     2281 gacgtcggtc aacaggaggt ggatcggacc cgggtgcgcc tcggagagcg cgatagcctc
     2341 ttcgccgcag gccgcctcga ggacagtata gccgcacatc tcgaggattt cccgcgcgag
     2401 ggcccgcacc tcttcttcgt cctccgtcag gagaatcgtg cccgagcccg tctcgccgcg
     2461 caggcccggc ggtggggcgg ccagtgtcgt cggctcgagg accacttcgg gcatgtacac
     2521 cttgaaggtc gttccctggc cgatctcgct gtacacctgg atgtcgccgc cgtactggcg
     2581 gacgatgccg taagcgatcg ataaccccag tcccgtaccc cgtccggcct ccttcgtcgt
     2641 gaagaagggt tcgaagatct tgagccgggt gtcggcatcc atgccgcggc cgttgtcgct
     2701 gacggaaagc tgcacgtagg cgccgggcgg cagcgaggga tgggcggcgg cgaaggtttc
     2761 gtccaccgtg acgcgcgcca cctcgacggt gatcttgcct cccgactcga tcgcatcgcg
     2821 ggcattcacc accaggttca tcacgatctg ctccacctgc ccggcatcgg ccctgatctg
     2881 agacggtgcg gtccccacca cggtcaccag ggcgatgtct tccgggatga gccggcggag
     2941 catggcgtcc aggtcgccca gcacacggtt caggtcgatg atcgtcggct cgaccagctg
     3001 cttccggctg aaggccagta gctgacgcgc gaggtcggcg ccgcgccggc ccgccttgag
     3061 gaccgcctcg gcctcccgat acagcgcgct ccccggctcc accttgcggg tcatcatctc
     3121 gcagtgaccg agaatgacgg tgaggaggtt gttgaagtcg tgagccacgc cgccggcgag
     3181 ccggccgatg gcttccatct tctgcgagtg acggagctgc tgttccaggc gccggctggc
     3241 cgaaacgtcg atggccacgc cgagcacgcc ctccgactcg tcccgcgagt tgcgcagcgg
     3301 ggagaaccag gtttcgtagg ccagggggcc cacgtccacg atctccttga actcgtggcc
     3361 ggccagcgcc cgccggttgg cgtcgcagat tgcgggatgg tcggcgtaaa cgtcgaagac
     3421 cgacttcccg accacctcgc cgggcttgag ccccagcttt tccaggccgc gcccttccga
     3481 catcgtgaac cggccgttgc cgtcgagcgt gaagaggacg atcggtgcgt tggatacgac
     3541 cgtccggagc tggtcctggc tgtgtcgcag agccgtttcg gcccgccgcc gctcggtgag
     3601 gtcccggaac tccacgacca ccaggttctg gtcgtccagt tccagcggcg agagaccgac
     3661 ctccaccacc cgctcctccg aatcaccggt ccaggcgatg gcctcgaacg tcggcgtggt
     3721 cgtcccggcg aaagcgtccc ggtagatccg ctcgaggcgc gcccactcgc ccggggagtg
     3781 gagctggtcg agcgtcatgg caaggagctc gtcggtcgtg tggcccagga ggcgacaggc
     3841 cgtgtcgttg gcgaggacga tccggagcgt tccgggatcg agcatcagga tggcgttgcc
     3901 gcttcggtcg aacagggttc ggtagcgggc ctcgaggcgg cggacgatct cgaggtcgga
     3961 gaggtcctgc acgaggacca ccgcctcttc gggccgtccg tcaggaccga ggatgggaag
     4021 ggccgacagg taaagttcgc ggacgacgtc ccccgtccga tggtgaatct cgcgatgagc
     4081 cgcctcgttg tcgcggagcg cccgggccac cgggcaatcg agcgcgcccg ggccgcagct
     4141 tgcgccggcc aggtgagagc agaagtccga gcacttccgg ccatgccagc cgttcgggtc
     4201 gcctccggct tcgcagtcga ccaggtggcg gttcgtccac cggatcgtcc gatcgacgtc
     4261 cgccacgaac acctcggcat cggtgcgatt gaccacggcc aggaatttct ggcgctcccg
     4321 gtcgagaacg cgaagctggc cgtcgagtcg cttgaaccag cgctcgtact gcgccaccac
     4381 ggccggatcg gttccgggag gaacttcgcg acttccgggc aacggcggcg tcatgcggct
     4441 ccggcttcag tcccgcgtga ccggcgctcg taaaggagcg ccaggcggtc ggccacaccc
     4501 tgcacgagcg caaccgccgc gtcgtcgaag gcagcgagct gatggctgtc gatgtcgatc
     4561 tgtgcgtaga tctggtcgcc catccggatc aacaccacga gctcggatcg ggtctgcgtg
     4621 ctgcaggcca ggtagttgct ctcggcccgc acgtcgggaa tgttcatgtt ccggcgttcg
     4681 gccaccgccc ggccgcacac gccgcgcccc acgccgatga agacatggtc ggtgggcgca
     4741 cccacgaagg ggcccagccg gagcacgtcg tccggaaaga gctcgtagat ccccgtccag
     4801 tggaagaggg gattggtcgt ggacagtttc tgcatggtcg cttcgaggat gtcctcgagg
     4861 tcgagtccct cggcgtccat gcggtcgatt tcgagcagga gatcggacag ggtcatggcg
     4921 gcactcctcc ggccggccgg cccggcacgg gtcggtccac cccggaggta tcggtccggg
     4981 ccgggatcgt ctttaatccc ggcatgatcg gcctcgtgct gaacccggaa gagggccccg
     5041 gccgacccgt ggccagcgtc ctctggatcg aggaccacga gctggaggag tgggtccgga
     5101 atggccgggt gacgcccgac acctgggtct gggccggccc ctggtccggt ggccggtgga
     5161 agcgggcgga cgacctcgag gtgtaccacc tcttccgccc cgagtccgtc gaggtgcgcc
     5221 cccccggcat atcactccgc gatacggtct ttccgaccag ggggctgtcg gccaccgagt
     5281 cgctgatgct cctcaacatc gcggtgagcg ccattctcct ggccgtgtgg cgggaggctt
     5341 acgaaaccga actctggcag acgatggcct ggtggtggac gcgggtgacc accgatctcg
     5401 aatggtggcg ctggctcccg acgctcgtga tgcacgccga tgcgggccat ctcctccgca
     5461 acctcggggc gctgctggtg acggcagggg gcgtcgaggt gttcttcggc cggctcagga
     5521 cgtggttgtt ctacgtggcg acgggggtcg cgggggcggt ggcgtcgttc ctgggccgcg
     5581 ccgaagcgcc gctctcggtc ggcgcttcgg gagccatctt cggcctggcg ggcgtcctca
     5641 cgggattcct tttccggcat gccggtcgat tctcggagcg gcagcggtgg aaggcgcgtc
     5701 gcgtctatgc gccgctcatc ctggtcctcg tgccgccgtc actctttcat gccgattggc
     5761 gggctcacct gggtggctat gtgctgggcg tgatactggg attggtgacg ccgctggggg
     5821 cggcgggcag gcgagtcctc gccggcaagg agatcagttc cgggtcaggt tcttgatgcg
     5881 gctccatgtg gcgtcctcga cggggaccgg gatgaccggg ccgttgtcga atgtcatggc
     5941 cgccagcgat cctgaacccg ggagagtgat gacagtccga tcgatcgccg tggtggtttc
     6001 cgtcagtacc tccacctggg agttgtcgcc gagtgccggt accgggaacg actggacgag
     6061 ctggttcccc ttgtagcaat cgacaaaggc gccggactct tcgatgtcga ggaggaccac
     6121 cttgagcagg aggccctgaa ccgtccagtt gacggtgatc tggccaccca ggtggttgtc
     6181 atccggggta tcgacgcggc cgtcctggtt cacgtccacc aggttctcgg ggatgaccaa
     6241 taccttcccc tgcggggtct tgttctgtcc cggcatcccg ggcttgccgc cgttcccctt
     6301 gccaggtcca ccgaacccat cgttgggggt tccgaggtcc aggtcggccc cggtggggtg
     6361 ggccgagtcg aaaatgcaga gggaattgga ggccgcgttc ccgatgaccg agaactcgaa
     6421 gccggggtag ggatcgctgg caaggatctg cccgttcggc ttgaccccgg caacgatggt
     6481 cccagcagca agtccttcaa atgtatatgg ttgtggaact gcagatgccg gggaggcgag
     6541 caggagcccc aatagcaggg tagtgggaat gccctccgat agggcgcggt tgcgcaggcg
     6601 catggtgtct cccttcgtca tgttttgtgc atgaagtatc aacaatcgtt gacacttgag
     6661 caattatccg acgggtgtcg gtacattgca agaggagagt aagaacgtgc agatatcaac
     6721 gctcctggcc ggtcggagga gtacccggtc cttcgcgcaa cgtgaagtgg ttcaggaaga
     6781 tattgacgct ttgtttgagg cggcccggtg ggccccctcc agcgggaacc gccagccctg
     6841 gcggtatctc tttcgcacct cacgggcggg ccaccccggg atgtcggcct gcctgacccg
     6901 ggggaaccaa tgggccctgg cggcgcccct cctggtcgtc cagctcaccc gggtggctga
     6961 cggcggggag ctgaatggcg ttccttatgc ctacgtgggg tgcggcctgt ccctgatgtc
     7021 gctgatcgtg gaggcggaat cgaggggctt gcgtgcccac ccgatggccg gatggatcga
     7081 ccagccgctc cgcgccgagt tcgacgtgcc ggcggactgg cagccggtcg tcgtcgtggc
     7141 tgtcggttac gaaggaaccc tcgatcagct cgatcccgcg cttcgggaaa aggaattgcg
     7201 cccgcggaca cgccggccgc tcgcggaggt catggtcgag ggtcggtggc cctcaggctg
     7261 gtcgggtccg ctttgacggt cgctccggcc agagtgccgc caggtcgagg gtggggcgcg
     7321 tggcgggtcg catgatccgg agagcgccgg gaagaagatc gatctcgatc ggcgtggtcc
     7381 gctcgatcag ttcaccgtcg atctgtaccg gaatcggccg gctggccacc acgcggatcc
     7441 gctcggcctg gtagaagcgg acccggggat cctgcttcaa cccgccgagg agcgcacgga
     7501 cgaagaggat caccagctcg gggagggtgg cgaacgtgaa gacgacgacg tcgagtcgcc
     7561 cgtcgtcgac gcgggcgtcc ggagccagcg ggagcgctcc catggtgcgc cccacgttgg
     7621 tcacgagcac cgacttggcg aggacgtgga ccttctcgcg gccgtccatc tcgatgatgt
     7681 agcgtgcccg cttcaccctc aggttcttga acatggcgat gagataggcc agcggcccga
     7741 aacggcgctt gagctcgcgg tctgcgtcgc ggatcaggtc ggcgtcgtag ccggcgccgg
     7801 cgctcagcac gaagatgcgg ccgtcatcca ggcgccccac gtcgtatggg agcgcggtgc
     7861 cgaaccaggc cgcgcgcgcc gcgtcgcgcg gggccatggg gataccgagg ttaatggcca
     7921 cgatgttccc cgtcccggcc gggatgacgc cgaggggaac gggacttccg accaggccgt
     7981 gcgccgcatc ggccagcgtg ccgtctccgc cggccaccag gacgagctgg tagccggcgt
     8041 cgagtgcggt acgcgtcttg tcgcgtgcgt gaaccgtgcc tcccacctcg atgaagtcga
     8101 tgaccgcccg cgtctcctcg agtcccttct ggatcccttc gcggatcttc tcgacgtccc
     8161 ccttgccggc accggcgttg agcagcgcca gcaccttgac cgggccgggc ccgccccact
     8221 cgggttcggg cggaggtgct tcggcggcgg gcgtacgggg atcgtcggcc atcgatcgct
     8281 ccgtctcgcg gacggtggtt actgtcccgg cgcgcccggc gcctcgggca acgcgaagct
     8341 cccgaccagc gccaggactt cggtcgggtc ggaggtcggc ggcgcacaga gttcaccgga
     8401 gcagacgtag gccagcggct tgccgtcttc cgacgcggga tagaggaggt cctccgcgcc
     8461 cgccgaacgg acctgcacga gctttcccgg gcgccaggcg cggacggcgg cgtcgcgcag
     8521 ctggcgaatc ccctcgctgg tcgagtcgcc gacgaggtac gccatcaccg atggccgaac
     8581 cgagagctcg agcgccagcc ccgccgaggc ccgtgcggcg aagaggggca tgacgcgtgt
     8641 ttcgggcgcc gtggccagtt gtaatggctc gagtcgacga ccggcctcct cgatgagcga
     8701 atccgcgtgc gtccacatgg cctgccggat gagatccagc gccgaacggg cggtggcgat
     8761 cgaatcggcc tgcgtgacgg gggtgacggg aggggccgga gccggaagcg gggtcggtgc
     8821 gggatttccc cctcccactc cccaggccgg ctcgccgaac acgagggcgg cgcgggccag
     8881 gcgctcgtcg gcgaggcgca tttcggcgtc cgacgccatt ggtccggcca gcgtgtcggg
     8941 ctcgttcgat gtccaggcga gtaccccgtc cgcggtgcgg ctccaggcga ggaggtcgcc
     9001 cgccgtcgag cgggcggcct ttcggaagag ctcctttcca ctggcctgga aggccatcgt
     9061 gaagagctcc agcagctcgg actggccggt cagagtcttg accttgcgcg cctcccttcc
     9121 gttcgccggc acatcggttc gggtggagtc ggcgacgccc cacttcaggt aatcgttgag
     9181 gtggaggccg acccaggcgc cggtggcgcc cagcgcggca tcgctccagg ccttttcgcc
     9241 ccgatgtgcc ccgagggtgt acatgaattc cgccgagcgc ggttcgggga gcggcgtcat
     9301 cgacccctgg atgtaccagt gggccagcat ggtgagacca tcccgcgtct ggatggcgag
     9361 gtggtcgatt gcggcctggc gtggatcggt gtcggtggtg gcgagcgggg tgagatcgga
     9421 cgtggcgaac ggatcggtcg gtcccgcaac ggacgttccg ggaatcgcga aaccgagagc
     9481 cgccagggtc aggagcccgg ggacgggccg aagtcgtttg atcagcatgt atggatttcc
     9541 catcaggttg gcgaacaagc gagacggatg gtagctttcg acgcccggca aatccagttg
     9601 ccgggcggcg gccatgtccc gaccgggccg cgtggatttc gggaatcagg ccgccagggg
     9661 agagccatga ccggcccgtc gaacgaccgt ttgccgcgcc gggttccggt catcctcgtg
     9721 cgacatggtc tgaccgagtg gaatgatctg ggcctcatcc aggggtggac cgacattccg
     9781 ctctcacccc tggggcggcg gcaggccgag gccaccgcgc gggcccttgc cgggcgtccg
     9841 atctcggccg tggccagttc cgacctcgtc cgcgcccggg aaaccgccga ggcgatcgcc
     9901 cgcgcgcacg ggctcacgcc gacgctccat gccgaccttc gcgaatatca ctgcggcgag
     9961 tgggaggggc gtcccttcct cgaggtgcgg cgtgaccatc ccgacgcctt ccgcgcctgg
    10021 ttcaacgatg ccgaggcgcc catccccggc ggcgaatcga tgggcgccgc gctgcgccgc
    10081 gccgggcctg ccgtcgatgc cctcctggcg agcctcgccg gcgagggagc gctggtactc
    10141 gtcggccacg gcggcatcaa ccgggtcctg gccgcgcact tcctggccat gccgctggcg
    10201 cacgcccggc gtttcatgct cgataacgcc tcgatttcga tcttcgagcc ccaccactcg
    10261 gggtgggcgc tccgatcgtg gaatcacacc gggcacctcg acgcgctcgc cgacgaaacc
    10321 ggcggcgacg cgcgacggac cgacgcgccc cggaccaact gaagtgaacc gcttgtggcg
    10381 ccaattcgat cgcctcctcg aggggagggt attcccggct ctcctcctgc tgggagtggt
    10441 cctgcgcgtg gcggccattg tctcgttccc ggtctacccg ctcgtcgaca acacggcgga
    10501 caccgccatt tacgacgaag gcgcgcggtc gctcgcggcc ggcgagggat accgatgggg
    10561 agggcggctc accgccttct ttccgatcgg ctggcctctg ctcctctcca tggcctatcg
    10621 cgtggcgggc gaatcagcgc gcagcggtca ggcgctcaac tttctcttca gcctcgtgct
    10681 cctcctggcc tgctggtttc tggtgcatcg actggccggc cggcgggcgg ggcgccttgc
    10741 ggcgctcgtg ttggcgtttg cgccgcacca cgtggtctac ccggccttcc tcatgtccga
    10801 aacgtccttc accgccttct tcctcctgtc gctgggattg gtcgcggtgg ccgttcctcc
    10861 cggacgggag catggggaaa agctgccgct gctcatcggc gccggcgtgc tgatgggcgc
    10921 cgccacgctg atccgggggc cggcactcgt ctttcccctg gccgtcgcgg cgtggacctt
    10981 cgtttcggaa cgtgggcggg cggtgccccg catgctgcga tcgatgctgt gcgcgttcgt
    11041 gttcggcctg ggcctcctcc tggcggtggc cccgtgggcc acgcgcaacc accgcgtgtt
    11101 cgatcgatgg gtactcgtgg ccaacgacgg tggcatgaat ttcctgatgg gcaaccatca
    11161 cggtgccacc ggggcacggc acgaaccggt ggaggggttg cccgacctgg ggaacgaggt
    11221 ggccgatgat cgggaggggt accggcgtgg cgccgccttc atcaaggaaa acccgggcga
    11281 attcctcacc ctgctgccga agaaattcgt gcgactcgtc gcgccggcgt cgctcctgac
    11341 ctaccgcgcc gagctgcgcg ccaagtggcc cgagcccctg gcgcttaccc tcctcgccat
    11401 cgaccagctc cttcatgccg ttttgtggat catggcgctg gtggccgtgc tgaagctccg
    11461 aagtcatccc gcggtccggt tcggcgcggt catgatcggc ctgtgggtgc tcatccacct
    11521 ggcgtttctc gggggcgccc gctacttcta cccggtgatg ccgctcctcc tgctcgtggc
    11581 ggcgagcgcc cggttcagcg aacgagaagc ttgatgccgc tccaggtggc ggggcgcacg
    11641 acggtggcgc acgccacgcc ggtgcacccc acgccgatcc ccttgaccac cgggcaattc
    11701 cccgaggaga cggccgccgg cgagttggta cagacgcggt aatcgccccc ctcggcattg
    11761 cagaacatgg gtgggccgaa cacgtcggtc tcgatcggcg ggcacccctc cagcggtggg
    11821 gaggcgttcg tgccgaagag gttgcacccg ccggcatata tcgccccgtt ggcgcagccc
    11881 aataccgaga atgcgccacc cgtctggccg tcgaaaacgt tggcgttgac ctcgatggtg
    11941 gtgccgtcgt acagggcgag cccggagcca tggcggtcgc agcggttctt cacgaaggtg
    12001 ttctggaaga tgcggatggt cggcggcggc agggccagtc ccgtcatgat ctggagcccg
    12061 ccgccgtcga ggatggcctc attgtcgacg aacagcgaga gggacaccga gccgacggcg
    12121 ttgatgatgg cggcgccgcc cccctggccg gagaagttgt ggcggaaagt gcaattgacc
    12181 acccggaagt aagcctggtc gaagcagacg ccgccaccct ccgaaccgcc gaagttgttt
    12241 tcgaaaaggc agtcctgcac caggatctcg tcccgcggac cggcgacctg cgcacgccgg
    12301 ggccccgtgg accggctccc tccacccgcg tgggacaact ggatcagggg gagcgaggtg
    12361 gcgaataggc cgcctcccag acttgagcag gagttgtcga ggaaccggca gccgatgatc
    12421 gcgaagttag cctccacgcc gaggacgccg ccgccatact cgccaccgtc gatgtcctgc
    12481 ggggagttgg cattgccgtc ccggatcgtg agatcacgca cttccgagta gttccccgtg
    12541 ccgcggatct ggagggtgtg gccgtgttca cccacctggg caccaccgtc cagggtggtc
    12601 cgctcgacgc cggcgccgaa catgtacagc gtcttgttga cgatccggct ccgctcgctg
    12661 taaatcccgg tggccaggag cagcgtgtcg ccatcggccg cggagtcgat ggcggcctgg
    12721 atggaaacgg catcgccgcc acccggggtc agatgccagg tgcgggcccg ggcgggtggt
    12781 gagcccgtta gcatcgcagc gagggccagc acgacggcca gcgcgcacgg gccgcgcgcg
    12841 cgccggcaac aggggagtgg aatggcagag gcaggtgtca tcgagacgat cctaagcagc
    12901 gcacgcggga gccgtccagc cgcgcgcgtc gttttccgct cctggcagcc gacggatccc
    12961 gttgcggccg tcatcctgct ccacgggctc ggcggacatt cggatcgatt ccaggagtgc
    13021 ggcagtttct gggcgtcgcg tgccctcgcg gtgtacgccc ccgatctccc gggcttcggt
    13081 cataccgacg gcccgaaggg acacctggac tcgttcgagc cgatgttcac caccatcgag
    13141 gtgatcctgg cccgggtgat ggccgaccat tcccgcctcc cggtcttcat cgtggccgag
    13201 agcatgggag cggccgtggc catcgactgg gtgtcgcgcc atccggcgct cctctccggc
    13261 ctcgtcctcc tggcgcccgc gttgaaagac cgactgaacg tgccggtctg gcggaaggtg
    13321 gaagcactgg tccacgtgat cgtgcggcgg cgcaagtact acgacgtgcc gttcgagccg
    13381 gacaacttca cccgggacgc ggcgatcaag gaagtgctgg cggccgatcc gctcgaggtg
    13441 cggcgggtta ccgcgcagtt ctacttcgcc tggctgccgg tcaccgcccg cgccctcaag
    13501 gcggcgccgc tcctcaagct gcccgtggcc gtcctgcttc cgggcaagga cacgatgatc
    13561 gactcggatg ccacgcgagt ctggttcgaa ggactccccg aggccacccg gacgctcgtc
    13621 gaatatccgg ggctgctgca cggcctgctc gtggagctcg atcgccagcc ggtcctggac
    13681 gacatcgcgg cgtggatccg caagcaggtc ggccgctgat ccggcccccg cttgggatca
    13741 cggcgacgga ctggtagcat cgcccgccat gctcccaatc cttttcgaga tcgggccgtt
    13801 caagctccac tcgttcggcc tcatgatggc cctggccttt cttgccgcca cgctcatcgc
    13861 cgcccggcgc ttccgtcgac tcggcctcga ccccgaccgg gcgagcgagc tggggctcgg
    13921 cgccatggtg ggtggcgtac tcggcgccaa ggtctactac ctcatcgacc actggtccga
    13981 caccgcggcc gacccgatga agatgatctt ctccggctcc ggcctcacct ggtacggcgg
    14041 cctcctgggc ggcgtgatcg ggacgctggc ggtggcgaaa tggcgcaagg tgcccctcct
    14101 ctggctgtgc gacctggcgg ctccgtcgct cggcctctcg tacgccatcg gtcggatggg
    14161 atgcttcctc aacggcgacg actacggtcg cccgacggac gtgccgtggg ccatggcctt
    14221 tccgaaggga acaccgccga ccaccacgcc ggtccacccc acgcagctct acgaaaccgc
    14281 cgggggtctc ctcatcttct gggtgctgtc gcgcctcggc gacgaccgcc tggggacggg
    14341 gctcatcttc ggcctgtatc tcctgatggc cggcttcgaa cggttcgtgg tggagttcta
    14401 ccggacgaac gaacccctgg ccgccgggct caccctggca caatggatca gcgtgggcct
    14461 gatgctggcg ggggtcgtct tcgtcagtcg cgcccgggcc gggcaacgga aaggtctggc
    14521 caccgccgcc acggcgggcg gttgaacggg ccgcacggct cgtccacgaa ggcgcccatt
    14581 ccgccgcagg aggccagtcg ggccgggctg tcggccttga agtggaagtc caggtcgtcc
    14641 ggcccgcaga atcccgggtc gacggagagg tttcccatcg tcccggtcac atccgggcat
    14701 ccgtgatagt tgcccgatga gtgctgcacc acgtcggagc agcggacctc cgccgaaccg
    14761 cccgtccggc agtcgatgcc gaagccgcgg ttgtgggcga tgaccgagcg ttcgatgacc
    14821 gcgtgggcgc cgccggccac cacgacgccg gtggagtcgt cgggaaccat gccgacgccg
    14881 gtcaccgtga cgttcctgag ccggaccacg ccaccttcct cgatccggat tccggtgagg
    14941 cagcagaggg cgatgagcgt gtgctcgagc gtgaagtctc ggctttcggc caggtagggg
    15001 ccgtaggtgt tcccccagaa ctgggagcgt gtcatccgtc cggtgcagtt gcggaggacc
    15061 atggcctccg cggcgtattc catgaagagg gaattccgga tatcgagttc ggcgtcgtcg
    15121 gccacgatgc catgctgccc cgaaacgaac tcgcactcct cgatcatgac cgtgccgccg
    15181 gcggcccgca gcccggcgtc gcagttgtca aagcggatcc cccgaaccac caccgtgtcg
    15241 tcgccggcgc ggacgtggag cccgaggctg cccggccgcc acatgccgtt gacgcggaca
    15301 tcggcgcccg gggcggcacg cagcacctgt gagcggcggg cggagaggat ggcgctcacc
    15361 acgcccccca tcccgtcgtc gaccagcgtg tcggccggcg tgtcgtaccc gccggccgcg
    15421 aactcgatcg tgtcgccggc cgcggcggca tccaggcact cctgcggcat tccgcagtcg
    15481 gagccggtgc cgtcgggcgt ggtgtgccag gtgcggtatc cctcgggagt cgggcccacc
    15541 ggatcgtcgt cgccgccgca gccgccgaat ccggccgtga tggcgaggag gagcagggcg
    15601 agcctcagcg gccggaaccg gcgtagcgtt gtgttatcgt cgcgtggtga caagcggagt
    15661 cttccgggtg cgaaacgagg tcgcgaagga atcggacatg gttccgagcg taacgagccc
    15721 ggctcattgt aacccggccc gccgaaccgt gttcgtccgg gtgctcgtcg ccctcttcct
    15781 tgcggtggcg ttcgcgccga acgcccgcgc acaggaccgc tcaccggccc cgggccctcc
    15841 cggtgtttcg cggggcggac tcaattacgc ggcggagctc tcggtccggc gtaacgccgc
    15901 gggcgccacg gtcctcgatg tcctggtcga ggtgccgtat tcgtcactcc gtttcgcccg
    15961 tgatgccggc gggtgggtgg cccgcttcga cgtgaccgtg ctggtgtacg accggagcgg
    16021 gaaccaggtg aatggcgatc tctggaccat cccggtgcgc agcaccgacg cggcccgcga
    16081 ccaatcccgg ggtctctcga tccggcgtcg ctatcccctg atcgtgaccg aggggcgcct
    16141 caaggtcgac atctcggccg tccagtcggg gagcgggcgg gagggcacct ggacgcggac
    16201 gatcgaggtg ccgcgctggg acggaacgga gatggcactg tctcttccga tgttcggccg
    16261 ctgcgatctt tcgaccgcca cacgcgacag ttcctggtcc cctcccgaca cgagctgggg
    16321 gcccgctttt gcgccggtgg tccgccgtcg ctacggcgac agccagcccg acctgtgcgt
    16381 ccggggcgag gtttacgacc aggatccttc cgacgcgcct gcgtaccggt tcgactggtc
    16441 gatcgagggt gagcacgacc gcaagggtgg atcgggatcg ttcgaggtgc cgcgcgtgaa
    16501 ccaccgcggc acctggatgg tgcatccccc gatcgattcg ctcagccgcg gcgagtggcg
    16561 gctcttcgtc acggcccgcc tcgccaaccg gtccgtccgc atcgaggaac ggttcgagat
    16621 cgacgaatcg cgcctcaacg tgctcgacga cgccgacatg atccgcggcg tcctggccta
    16681 catcgccgac aacgaggaac tggtgcgcct cgagaacctg ccggtcgaca gcctggccac
    16741 cttctgggac cagttctggc ggcgccggga cccgtcgaag gacacgcccc gcaacgagaa
    16801 ccgcgatgag ttcatgcgcc gcgtcgagta cgtcaatgcc ggctattcca tcatggacgc
    16861 aggatggcgg tcggacatgg ggcgcatcta catccggtat ggcgcgcccg accaggtgga
    16921 gaaggtgccg tatacctccg aaggacctcc gcacgagatc tggtactaca ccgagcgcaa
    16981 cctccgcttc gtcttcgtcg attccgaggg attcggccgc ttccgactgg tggggcgcga
    17041 acggccatga cccggttggg gaagcggctc atcgaggtga gcgtggcggc catccagaag
    17101 gagccatcgc acgccgccga gctggtcacg caggcggtga tgggcgtgcg cgtccgcgtt
    17161 ctcgacgagg cggatggtgg tcgatggctc cgggtggtcc tccccgacgg ctatcgcggc
    17221 tggctccgcg cctggctggc ggcgcccgac gtggccacct ggcccggtcc gcgcgtggcc
    17281 gaggtcgatg tcccctggac ctgggtcctg ccggctcccg ggcggcacga cgagccggtc
    17341 tccgatgtcg tgattggaac acggctggcg gtcctcgggc gggccgatgg ggggtggcaa
    17401 ccggtggccc ttcccgatgg
//
LOCUS       DASVOD010000249         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_851512, whole genome shotgun sequence.
ACCESSION   DASVOD010000249 DASVOD010000000
VERSION     DASVOD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851512"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /gene="murD"
                     /locus_tag="VF720_05380"
     CDS             <1..195
                     /gene="murD"
                     /locus_tag="VF720_05380"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543887.1"
                     /note="UDP-N-acetylmuramoylalanine--D-glutamate ligase;
                     involved in peptidoglycan biosynthesis; cytoplasmic;
                     catalyzes the addition of glutamate to the nucleotide
                     precursor UDP-N-acetylmuramoyl-L-alanine during cell wall
                     formation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7878818.1"
                     /translation="SDWVSAGTSFERAVEAAYQEALARGGVVLLSPGCASFDMFKDYE
                     DRGRRFKALVAGLGAERIGS"
     gene            192..1478
                     /gene="ftsW"
                     /locus_tag="VF720_05385"
     CDS             192..1478
                     /gene="ftsW"
                     /locus_tag="VF720_05385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEX7878819.1"
                     /translation="MTERILPFARKGFDSGRRAIREIGTSMLEEPIVNDRSAIVLPEE
                     MRNERVDWALLLATLFLFGLGMLMVLSSTSFVAQTAHKDPFGRFANQGIRGLIGLVFM
                     IGAMKLDYRRLSGWAPVIAVGSAMLLLLVFLPGVGETSKGSQRWIGLGPVRLQPIELA
                     RLGLIIYLARLLSKPQARITRFTTGPLPAYLAAGVFMALIIIQPSLGSTLTLALTTLA
                     MCLVAGMKRKHFLLTCLAGFAGLVLVLILKHDSYQVQRLLGFIKIWTNDPDRLGVTYQ
                     VRQSLIALGSGGFSGEGIGNSAQKRFFLPDAHTDFIYSIIGEELGFIGTIGVLALFVI
                     LIWRGLRIAGQAEDRFGYLLAAGLTINFMIYATINMGVTMALLPVTGLPLPFVSYGGS
                     ALIANLAAVGVLLSISRRCGTSFALIPARRHRKGLA"
BASE COUNT          235 a          564 c          530 g          288 t
ORIGIN      
        1 agcgactggg tgtcggccgg aacctctttc gaacgggcgg tggaggccgc ctaccaggag
       61 gcgctggcac gcggcggggt ggtgctcctg tcccccggct gcgcctcctt cgacatgttc
      121 aaggactacg aggaccgagg ccggcgcttc aaggcgctgg tggccggcct cggcgcggaa
      181 aggatcggat catgaccgaa cgaatcctgc cgttcgcccg aaagggattc gactccgggc
      241 gccgggccat ccgggaaatc ggcacctcca tgcttgagga gccgatcgtc aacgaccgct
      301 cggccatcgt cctgcccgag gagatgcgga acgagcgcgt cgactgggcg ctcctcctgg
      361 ccacgctctt cctgttcgga ctcggcatgc tgatggtcct ctcgtcgacg agcttcgtgg
      421 cgcagacggc ccacaaggat ccgttcgggc gattcgccaa ccaggggatc cgtggcctca
      481 tcggcctcgt gttcatgatc ggcgccatga agctcgacta ccgccgcctc tccggctggg
      541 cgcccgtcat cgcggtcggc tcggcgatgc tcctcctcct ggtcttcctg ccgggcgtcg
      601 gcgaaacgag caaggggagc cagcggtgga tcggcctggg gccggtccgc cttcagccga
      661 tcgaactggc gcgcctcggg ctcatcatct acctggcgcg cctcctttcg aagccgcagg
      721 cccggatcac gcgcttcacg accggtccgc ttccggccta cctggcagcc ggcgtcttca
      781 tggcgctgat catcatccag ccgagcctcg gctcgacgct gaccctggcg ctcaccacgc
      841 tggccatgtg cctggtggcc ggcatgaagc gaaagcactt cctgttgacc tgcctggccg
      901 gcttcgccgg cctcgtgctg gtgctcatcc tcaagcacga cagctaccag gtgcagcgcc
      961 tcctgggctt catcaagatc tggacgaacg accccgaccg gctcggcgtg acctaccagg
     1021 tgcgccagtc gctgatcgcg ctcggctccg gcggtttctc cggcgagggg atcggcaaca
     1081 gcgcgcagaa gcgattcttc ctgccggacg cccacaccga cttcatctac tcgatcatcg
     1141 gcgaggagct cggtttcatc gggacgatcg gcgtcctcgc ccttttcgtc atcctgatct
     1201 ggcggggcct ccggatcgcc gggcaggccg aggatcgctt cggctacctg ctggccgcgg
     1261 ggctcacgat caatttcatg atctacgcca ccatcaacat gggggtcacc atggcgctcc
     1321 tgccggtgac cggcttgccg ctgccgttcg tttcctacgg cggctcggcg ctgatcgcca
     1381 atctagcggc ggtcggcgtt ctgctcagca tttcgcggcg ctgcgggacc agctttgcgc
     1441 tgatcccggc ccgccgccac agaaaaggac tcgcgtgagc gaacgggaag aatcgccccg
     1501 gcccgagcgg ccgtcggacg acgggaccag gcgtccctcg cgccggcgtc gccctcggcc
     1561 gtaccggggt caacagggaa ttgaggcgcg gccggcgcca cgtggtggtg aggtgga
//
LOCUS       DASVOD010000250         6660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_225717, whole genome shotgun sequence.
ACCESSION   DASVOD010000250 DASVOD010000000
VERSION     DASVOD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225717"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VF720_05390"
     CDS             <1..438
                     /locus_tag="VF720_05390"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878820.1"
                     /translation="SRDRLEETLFDELIARNPFDLPGSIVRSALDDVVERLKRERGGQ
                     DIPAEEVAQVREYYKPAVDRRIKSDLIIAAAGRLLEVQILDEDVDNEIARYAEREKTT
                     PAEVRGKLKKTGGLDRLRDDMYRHKVIETLLGQARVDVVKKGR"
     gene            442..1032
                     /gene="clpP"
                     /locus_tag="VF720_05395"
     CDS             442..1032
                     /gene="clpP"
                     /locus_tag="VF720_05395"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX7878821.1"
                     /translation="MALVPMVVEQSARGERAYDIYSRLLKDRIIFLGTAIDDIVANLI
                     IAQMLFLEAEDPDKDIQLYINSPGGSVTAGLGIYDTMQFIKPHVATTCMGQAASMGAI
                     LLLAGTAGKRSILPNARVMIHQPLGGTQGQAADIEIYTKEMLLIRDRLNSIINNHTGR
                     TLEQILKDTDRNYFMSAAEAKEYGIVDEVIVKRGQA"
     gene            1096..2340
                     /gene="clpX"
                     /locus_tag="VF720_05400"
     CDS             1096..2340
                     /gene="clpX"
                     /locus_tag="VF720_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX7878822.1"
                     /translation="MPSPHPIKCSFCGRGQEEVSRLVSGPSVYICSECVKLCNDILDG
                     ETDRDLPAHRESLPKPSEIKRVLDEYVIGQERAKKTLAVAVYNHYKRIFNPAGRDEVE
                     LDKGNILLIGPTGTGKTLLAQTLSRFLKVPFAIIDATCLTEAGYVGEDVENILVRLLQ
                     AADFNLVNAEHGIVYIDEIDKISRKSENPSITRDVSGEGVQQALLKILEGTVANVPPQ
                     GGRKHPQQKYIEVNSKNILFICGGAFEGLEKIVEKRTHQQTMGFGAQVTGKNKKNVGE
                     LLAQVEPEDLLKFGIIPELVGRLPVVATLDSLSEDALVDILTKPKNAITKQYGRFFDM
                     ENVRLSFEPGALTAIAQQALARGTGARGLRAVLEDAMLDLMYDIPSQADVELVTITAD
                     VINKKAAPAITWRNERRKKEAS"
     gene            2361..4748
                     /gene="lon"
                     /locus_tag="VF720_05405"
     CDS             2361..4748
                     /gene="lon"
                     /locus_tag="VF720_05405"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008515521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX7878823.1"
                     /translation="MQIKRDDEIFEIEDRLPLLPLRDVVLFPYMTTPLLVGRAGSVNA
                     IEACVQRDRILFATAQMKPEMVDPAREDVHKVGTVIRILQLFRLPDGTMRVLVEGICR
                     ARVKRFVASEDYFQVKLELMPDEVDKSAEVEALMRSTLAQFTDYVHLNRRISDEVLVT
                     VNNIVDPHILAYTIANHLVVKVPVKQEVLELDDPMLRLKRLSEILLSELEIVKLERKI
                     EGQVRSQVHKNQKEFYLNEQLKAIRKELGYQNEFASEIEELERDIKKARMPREVQEKA
                     VRELDRLSKMSFMSPEATVLRNYLDWLVALPWKKRSNDNLDLASVQKCLDEDHHGLKK
                     IKERILEYMAIIKLSKSLKGPILCFAGPPGVGKTSLGKSIARALGRKFVRMSLGGVKD
                     ESEVRGHRRTYIGSMPGRIVQAMKKAGTVNPVILLDEVDKLGSDWRGDPSAALLEVLD
                     PEQNNAFNDHYLEVDYDLSQVLFITTANYLGNIPPPLRDRMEIIQLPGYLENEKVQIA
                     RDFLVKKQAAASGLKEGDVVMSDKTLGDIVMQYTREAGVRSLEREIATITRKVARKVA
                     GGEGGVPVTVTSAQLAKYLGPPKFLDQPLEQERRIGLANGLAWTEFGGDVMTIEVSIL
                     PGKGKLNLTGKLGDVMRESAMAALSYARRISGFLGLEKNFYQNIDIHIHVPEGAVPKD
                     GPSAGITMAVALISALTGIPTLPHVAMTGEITLRGSVLPIGGLTEKSVAARRFGMKTL
                     IVPAGNRKDLEEIPKEIRQGLDIVIVEHMDEVLEHTLAKTEKSPDFRQTRNLTAH"
     gene            4840..5937
                     /locus_tag="VF720_05410"
     CDS             4840..5937
                     /locus_tag="VF720_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEX7878824.1"
                     /translation="MALERLFTPGPTPIPERIRRVMAEPIIHHRTAEFTEIFRGTSEG
                     LKPLFGTTEPVITLAASGSGGLEAALVNCCSSGRTLVTVNAGKFGERWSDLGKAYRLD
                     VAELTVPYGQVATPDQLAAFLDAQPHTVQAVALTHSETSTGALHDVAELAAVARDRGA
                     MVLVDAITSLGVHPVRMDEWGLDAVVSGSQKGLMLPPGLAFVALSERARHAMAGADLP
                     RFYFDLAEAARQLSKGTTPFTPAIGLVVGLAESLRMLNEEGLDNVFARHARLAAACRA
                     GMTRLGLPLFADSPSNGVTAVGSLAEPFPEQVVKTLRSAHGMRLAGGQGSMKGKIFRV
                     GHMGAYTDQDIQDVLAALSNVMTTLGAPVRS"
     gene            5934..>6660
                     /locus_tag="VF720_05415"
     CDS             5934..>6660
                     /locus_tag="VF720_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEX7878825.1"
                     /translation="MIKVVVSDPMAKDGLDILRASGLFDVTTVDGGDAAALKSALSDA
                     AALLVRSGTKVTADVMAAAPALKLVGRAGTGVDNVDVEAATRRGIVVMNTPDANSIAA
                     AELTWTLLLALVRNVVPAATSMAAGKWERSALMGTELAGKTLGVVGFGRIGREVAARA
                     RAFDMTVLVSDPFVTDEIARAAGARLVALDGLLAASDVVTLHLPLTKGTRHLVGASQL
                     ASMKPGARLVNCARGGLVDEAALK"
BASE COUNT         1256 a         2237 c         2157 g         1010 t
ORIGIN      
        1 agccgcgacc ggctcgagga gaccctgttc gacgagctga ttgcccggaa ccccttcgat
       61 ctgccgggtt ccatcgtccg ctcggccctc gacgacgtgg tcgagaggtt gaagcgggag
      121 cggggaggcc aggatatccc ggccgaggag gttgcccagg tccgcgagta ctacaagccg
      181 gccgtggacc ggcggatcaa gtccgacctc atcatcgcgg ccgccgggcg cttgctcgag
      241 gttcagattc tggatgaaga tgtcgacaac gaaatcgccc ggtacgcgga gcgggaaaaa
      301 acgacacccg ccgaggttcg gggtaagctc aagaaaaccg gcgggttgga ccgtcttcgg
      361 gacgacatgt accgccacaa ggtcatcgag acgttgctcg gccaggcccg ggtcgacgtg
      421 gtcaagaagg ggaggtaggg aatggcgctg gtcccgatgg tcgtcgagca gtccgcccgt
      481 ggcgaacgcg cttacgacat ctacagccgg ctgttgaagg accggatcat ctttctgggc
      541 accgccatcg acgacatcgt ggccaacctc atcatcgccc agatgctctt cctggaggcc
      601 gaggaccccg acaaggacat ccagctctac atcaacagcc cgggcggctc ggtgacggcg
      661 gggctcggaa tctacgacac gatgcagttc atcaaacccc acgtggccac gacctgcatg
      721 gggcaggccg cttccatggg ggccatcctc ctcctggccg gcacggccgg gaagcgctcc
      781 attcttccca acgcacgggt catgatccac cagccgctgg ggggaaccca ggggcaggcg
      841 gcggatatcg agatatatac caaggagatg ctgctgatcc gggaccgcct gaacagcatc
      901 atcaacaacc acaccggccg gaccctggag cagatcctca aggacaccga ccgcaactat
      961 ttcatgtcgg cggccgaggc caaggaatac ggcatcgtcg acgaagtcat cgtcaagcgg
     1021 ggacaggcct aggccgataa cccgagctac cggcgccttg ccggagggcc cttccccaag
     1081 gagatgaaaa agcgcgtgcc gtcgccacat ccgatcaagt gctcattctg cggtcgtggt
     1141 caggaggagg tcagtcgcct cgtctccggg ccgtcggttt acatctgcag cgagtgcgtc
     1201 aagctctgca acgacatcct cgacggggaa accgatcgtg atctccccgc acaccgggaa
     1261 tcgcttccga agccgtcgga gatcaagcgt gtcctcgacg agtacgtgat cgggcaggag
     1321 cgggccaaga agacgctggc cgtggcggtg tacaaccact acaagcgcat cttcaatccg
     1381 gccggccgcg acgaagtgga gctcgacaag gggaacatcc tcctgatcgg ccccacgggc
     1441 accggcaaga cgctcctggc ccagacgctc agccgcttcc tcaaggtgcc cttcgccatc
     1501 atcgacgcca cctgcctcac cgaggccggt tacgtgggcg aagacgtgga gaacatcctc
     1561 gtccggctcc tccaggcagc cgacttcaat ctcgtcaacg ccgagcacgg catcgtctac
     1621 atcgacgaga tcgacaagat cagccggaag agcgagaacc cgagcatcac gcgggacgtg
     1681 tccggcgagg gggtgcagca ggcgctcctc aagatcctcg aagggaccgt ggccaacgtg
     1741 ccgccccagg ggggacggaa gcacccccag cagaaataca tcgaggtcaa ctccaagaac
     1801 atcctcttca tctgcggcgg ggccttcgaa gggctcgaga agatcgtgga gaagcggacg
     1861 caccagcaga cgatgggctt cggcgcgcag gtcaccggca agaacaagaa gaacgtcggt
     1921 gagctcctgg cgcaggtcga gcccgaggac ctcctcaagt tcggcatcat tccggagctc
     1981 gtggggcgcc tccccgtcgt ggccacgctc gacagcctgt cggaagacgc cctggtcgac
     2041 atcctgacca agcccaagaa cgccatcacc aagcagtacg ggcgcttctt cgacatggag
     2101 aacgtccggc tctcgttcga gcccggggcg ctcacggcca tcgcccagca ggcgctggcc
     2161 cgcggcaccg gggcgcgcgg cctcagggcc gtcctcgagg atgccatgct cgacctgatg
     2221 tacgacatcc cgtcgcaggc cgatgtggag ctggtgacga tcacggccga cgtgatcaac
     2281 aagaaggcgg cgcccgccat cacctggcgc aatgaacgac gcaagaagga agccagctag
     2341 tccccgaggg aacacccgac atgcagatca aacgcgacga cgaaatcttc gaaatcgaag
     2401 accgcctccc gctccttcca ttgcgggacg tcgttctctt tccctacatg accacgccgc
     2461 tcctcgtggg gcgcgccggt tcggtcaacg ccatcgaggc ctgcgtccag cgcgatcgca
     2521 tcctgttcgc cacggcgcag atgaagcccg agatggtcga tcccgcccgc gaggacgtgc
     2581 acaaggtggg caccgtcatc cgcatcctcc agctcttccg tctgccggac ggcaccatgc
     2641 gcgtcctggt cgaggggatc tgccgggcgc gggtgaagcg attcgtggcc tccgaagact
     2701 acttccaggt gaagctggag cttatgccgg acgaggtgga caagtcggcc gaggtcgagg
     2761 cgctcatgcg ctcgacgctg gcccagttca ccgactacgt gcacctgaac cgccgcatct
     2821 ccgacgaagt gctggtcacg gtcaacaaca tcgtcgatcc gcacatcctg gcctacacca
     2881 tcgccaatca cctcgtggtg aaggtgccgg tcaagcagga ggtgctggag ctcgacgacc
     2941 cgatgctccg gctgaagcgc ctgtccgaaa tcctcctctc cgagctggag atcgtgaagc
     3001 tcgagcggaa gatcgagggg caggtccgca gccaggtgca caagaaccag aaggagttct
     3061 acctcaacga gcagctcaag gccatccgga aggagctcgg ttaccagaac gagttcgcct
     3121 ccgagatcga ggagctggag cgggacatca agaaggcccg catgccgcgt gaggtgcagg
     3181 agaaggccgt gcgcgagctc gaccgcctct ccaagatgtc gttcatgtcg cccgaggcca
     3241 ccgtcctgcg caactacctg gactggctgg tggcgttgcc gtggaagaag cgctccaacg
     3301 acaacctcga cctggcctcg gtgcagaagt gcctcgacga ggaccaccac ggcctgaaga
     3361 agatcaagga acgcatcctc gagtacatgg ccatcatcaa gctttccaag tcgctcaagg
     3421 ggcccatcct ctgcttcgcg ggcccgccgg gcgtcggcaa gacgagcctc ggcaagtcga
     3481 tcgcccgcgc gctcggtcgc aagttcgtgc gcatgagcct gggcggcgtg aaggatgaat
     3541 cggaagtccg cggccatcga cgcacctaca tcggctcgat gccggggcgc atcgtgcagg
     3601 ccatgaagaa ggccggcacc gtgaacccgg tcatcctgct cgacgaagtc gacaagctcg
     3661 gctccgactg gcgcggcgat ccatcggccg cgctcctcga ggtgctcgat cccgagcaga
     3721 acaacgcgtt caacgaccac tacctcgagg tcgactacga cctgtcgcag gtgctcttca
     3781 tcaccacggc caattacctc ggcaacatcc cgccgccgct gcgcgaccgc atggagatca
     3841 tccagctgcc gggctacctc gagaacgaga aggtgcagat cgcccgcgac ttcctggtga
     3901 agaagcaggc ggccgcctcc ggactcaagg aaggcgatgt cgtcatgagc gacaagaccc
     3961 tcggcgacat cgtgatgcag tacacgcgcg aagccggcgt gcgcagcctc gagcgcgaga
     4021 tcgccaccat cacccgcaag gtggcccgca aggtcgccgg cggcgaggga ggcgttcccg
     4081 tcaccgtgac gagcgcccag ctggccaagt acctcggccc gcccaagttc ctcgaccagc
     4141 cgctcgaaca ggagcggcgg atcggcctgg ccaacggcct ggcctggact gagttcggcg
     4201 gcgacgtgat gacgatcgaa gtgtccatcc tgccgggcaa gggaaagctc aacctcaccg
     4261 gcaagctcgg cgacgtgatg cgcgagtcgg ccatggccgc cctctcgtac gcgcggcgca
     4321 tctccggctt cctcggcctc gagaagaatt tctaccagaa catcgacatc cacattcacg
     4381 tgcccgaagg cgcggtgccc aaggacggtc cgtccgccgg catcacgatg gcggtggcgc
     4441 tgatctcggc gctgaccggc atccccacgc tgccgcacgt ggccatgacc ggcgagatca
     4501 cgttgcgcgg atcggtgttg ccgatcggcg gcctcaccga gaagtcggtg gcggcccggc
     4561 gcttcggcat gaagacgctg atcgtgcccg ccggcaaccg gaaggatctc gaggaaatcc
     4621 cgaaggagat ccgccagggg ctcgacatcg tcatcgtcga gcacatggac gaggtgctcg
     4681 agcacacgct ggccaagacg gagaagagcc cggatttccg ccagacgcgc aacttgaccg
     4741 cgcactagag acgccggtac gtcccatcgc tcataatccc cggcccgtcg acggagccaa
     4801 cttcgtcgac gggccgaatc ttttcaccgg gaggtcttcg tggccctgga acgactcttc
     4861 acgcccggcc cgacccccat ccccgagcgg atccgccggg tgatggccga gccgatcatt
     4921 catcatcgca ccgccgagtt caccgagatc ttccgcggca cgtccgaggg attgaagccg
     4981 ctcttcggca cgacggaacc ggtcatcacg ctggcggcct cgggctcggg cggcctcgag
     5041 gcggcgctgg tcaactgctg ctcgagcgga cgcacgctcg tgaccgtgaa cgccggcaag
     5101 ttcggcgaac gctggagcga cctcggcaag gcctaccggc tcgacgtggc cgagctcacc
     5161 gtcccgtacg gacaggtggc cacccctgac cagctcgccg ccttcctcga tgcgcagccg
     5221 cacaccgtcc aggccgtggc gctcacccac agcgaaacct ccaccggcgc gctgcatgac
     5281 gtggcggaac tggcggccgt ggcccgggat cgcggggcga tggtcctggt ggatgccatc
     5341 acctcgctcg gcgtgcatcc agtccggatg gacgaatggg gcctcgacgc cgtggtgtcg
     5401 ggaagccaga agggattgat gctgccgccc ggcctggcct tcgtggcgct ttccgagcgg
     5461 gcgcgccatg ccatggccgg cgcggatctc ccgcgcttct acttcgacct ggccgaagcg
     5521 gccaggcagc tgtccaaggg aaccacgccg ttcacgccgg ccatcgggct ggtcgtcggg
     5581 ttggcggaat cactcaggat gctgaacgag gaaggtctcg acaacgtgtt cgcgcggcat
     5641 gcccgtctgg cggccgcctg ccgggccggc atgacgaggc tggggcttcc actcttcgcc
     5701 gactcgccgt cgaacggggt caccgccgtg ggatcgctgg ccgagccgtt ccccgagcag
     5761 gtggtgaaga ccctccgctc ggcccacggc atgcgcctgg ccggcgggca ggggagcatg
     5821 aaggggaaga tcttccgcgt gggacacatg ggggcctaca ccgaccagga tatccaggac
     5881 gtgctggcgg ccctttcgaa cgtcatgacg acgctcggcg caccggtgcg ctcgtgatca
     5941 aggtcgtcgt ctccgacccg atggcgaagg acgggctcga catcctgcgc gcctccggcc
     6001 tgttcgacgt gacgaccgtg gacggcggcg acgcggcggc cctcaagtca gccctgtctg
     6061 acgcggcagc gctgctggtc cgaagcggca cgaaagtgac ggccgacgtg atggccgccg
     6121 cacccgcact caagctcgtc ggccgggccg gcacgggcgt cgacaatgtc gatgtcgagg
     6181 ccgcgacgcg gcgcggcatc gtggtcatga acacgcccga cgccaattcc atcgcggccg
     6241 ccgagctcac ctggacattg ctcctggccc tggtgcgcaa cgtggttccc gcggccacct
     6301 cgatggcggc cggcaaatgg gaacgttcgg cgctcatggg aaccgagctg gccggcaaga
     6361 cgctgggcgt ggtcggcttc ggccggatcg gccgtgaagt ggccgcgcgg gcccgcgcgt
     6421 tcgacatgac ggtgctcgtg tccgacccgt tcgtcaccga cgagatcgcc cgcgccgcgg
     6481 gcgcccgcct cgtggctctc gacgggctgc tcgccgcctc ggatgtcgtc acgctccacc
     6541 tgccgctgac gaagggaaca cgacacctcg ttggcgcttc gcagctggcg tcgatgaagc
     6601 cgggcgcacg cctcgtgaac tgcgcgcggg gtggcctggt ggacgaggcg gcgctcaagg
//
LOCUS       DASVOD010000251         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_537811, whole genome shotgun sequence.
ACCESSION   DASVOD010000251 DASVOD010000000
VERSION     DASVOD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537811"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF720_05420"
     CDS             <1..579
                     /locus_tag="VF720_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878826.1"
                     /translation="IVDPDRSASFARHLGAMIGEDNAEAVWHEALGRPDPERVEALTS
                     LGYLRGSAAESAPPIRRHPLDELPKWLDTQRSKSLIKLAFMRLNEERAGEAARLLDQA
                     ISLDKGFADAWFLRARARAETGDRKGALNDLHACLERDPKHVGARNELARLRSTPVDQ
                     AERDVPSRLPGGGGGTVVGSTMATDGAGGTQR"
     gene            complement(576..1304)
                     /locus_tag="VF720_05425"
     CDS             complement(576..1304)
                     /locus_tag="VF720_05425"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX7878827.1"
                     /translation="MNRPGALATAALVVMMGMATGGRAAVVRIPASHDNTLYAPAADT
                     LSSNGSGPYLFAGRNGQGNVRRALLFFPVADSLPAGAIIDSVELRFTVSQVSNATPRS
                     LLVRRVLASWGEGGSSAPGGAGARPEPGDATWQHRFFPGDEWTNPGGDLAPLPSAASD
                     PGVAGLHVLEGPGLVADVAGWLDAGINAGWAVLGDEFEDNSARRINSRENAGSDTRPE
                     LVVIYRLPGSPIRAATWSRIKTHL"
     gene            complement(1312..>3760)
                     /locus_tag="VF720_05430"
     CDS             complement(1312..>3760)
                     /locus_tag="VF720_05430"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX7878828.1"
                     /translation="IFTGRNNQALRRRALVRFPMAGLPAGAVVSSATLTLRSTTSGGA
                     TDVNVHRVLADWGEGTSNAAGSEGAGAAATSGDATWLHRFYNTLLWSAPGGQYQGTPS
                     ATASVAAQNPYAWSGPGMVTDVQAWLDGVATNFGWVLVGVENAGNTEKRYASRQYAAD
                     TSYRPRLTITYTVPPPDPTGACCLDDGTCDEQTETACQSLGGHWQGEGTACHPEGCPV
                     VLTPFVDALTVPPVAVPISGTVGGIATYDMRMVEFRKKLHRDLPPTTVWGYEGHYPGP
                     TIVATRGLPVTVNWINDLRDSTGALRTTHYLPVDQCLHGPDTAGTGARVVVHLHGGHV
                     PQSVDGHPDSTFLPGESRTYTYPNNQAAALIWYHDHALGTTRLNVMMGLAGGYVVTDP
                     VEGALSLPGGAYDLPIIIQDRRFHADGSLSYPAIWEEHFFGDVALVNGTVWPFAEVRR
                     GAYRLRLLNGSNSRTYILRLSNGLPFTVIGGDAGLLERPVRRDSLLITPGERIDTIVD
                     FSGVAAGTGIILTNSAPAPYPIGDPMHPALPAIMKFVVRPEAGYAWTPPGILRPPSAL
                     DPATADMARDFSLEKMADDCTGQMWTINGLHWDDITELPRLGDTEVWRFINRSGVVHP
                     MHLHLVHFQVLDRAPFDVVADTLVFGTPVTPDSALGGWKDTVPVLPGEAVRVIARFDD
                     YTGRYPYHCHVLEHEDHEMMRQFEVVDTLVTGLPETPVVPVPMRLEPGYPNPMTGVTR
                     IAYEVPREAFVSLRLFDVAGRQVATLDEGIRAAGRHVVVWEGRATDGARLASGLYFYR
                     LTVDGSGDLSRKLLVLE"
BASE COUNT          630 a         1250 c         1296 g          584 t
ORIGIN      
        1 attgtcgacc ccgatcgctc agcgtcattc gccaggcacc tcggggcgat gatcggcgag
       61 gacaacgccg aggccgtctg gcatgaagcc ctgggacgcc ccgaccccga acgcgtggag
      121 gcgttgacca gcctcggcta cctgcgcgga agcgccgccg agtcggcgcc ccccatccgg
      181 cgccatcccc tcgacgaact tccgaagtgg ctcgataccc agcgctcgaa gtcgctcatc
      241 aagctggcct tcatgcggct caatgaggag agggcgggcg aggcggccag gctcctcgac
      301 caggcgattt ccctcgacaa gggtttcgcc gacgcctggt tccttcgcgc ccgggcgcgc
      361 gccgaaaccg gtgatcggaa aggagccctg aacgacctgc atgcctgcct cgaacgcgat
      421 ccgaagcatg tcggggcgcg gaacgagctg gcgcggctca ggtcgacgcc ggtggaccag
      481 gcggaacggg acgtgccgtc tcgccttccg ggcggaggcg gtggcacggt ggttggatcg
      541 acgatggcga ccgacggagc cggcgggacc cagcgctaga ggtgcgtctt gatccgactc
      601 cacgtggcgg cgcggatcgg cgaaccgggg agtcgataga tcaccaccag ctccgggcgc
      661 gtgtccgacc cggcattctc ccgtgaattg atgcgacggg cggaattgtc ctcgaactca
      721 tcgcccagca cggcccatcc ggcattgatg ccggcgtcca gccagccggc cacgtcggca
      781 accagacccg gcccctcgag cacatggaga ccggcgacac ccgggtccga cgcggctgac
      841 ggcagggggg cgaggtcacc gcccggattc gtccactcgt caccgggaaa gaaccgatgc
      901 tgccaggtgg cgtctcccgg ctcgggcctt gctcctgcgc cgcccggtgc ggatgacccc
      961 ccctcacccc aggatgccag cacacggcgc accaggagcg atcgcggggt ggcattggat
     1021 acctgggata ccgtgaagcg aagttcgacc gaatcgatga tggcaccggc cggaaggctg
     1081 tcggccacgg gaaagaagag gagggcgcgt cggacgtttc cctggccatt ccggcccgcg
     1141 aacaggtacg ggccggaacc attgctgctc agcgtgtccg ccgccggggc atagagcgtg
     1201 ttgtcgtgac ttgcgggaat ccggacgacg gcggcccttc cccccgttgc catacccatc
     1261 atgaccacca gggcggccgt cgccagtgcg ccgggacgat tcatggttcc ctcattccag
     1321 caccaggagt ttcctggaca ggtcgccgga cccgtcgacg gtcaggcggt agaagtagag
     1381 gccggaggcc aggcgcgcgc catccgtcgc tcgcccctcc cagaccacca catgacgacc
     1441 ggctgcccgg atcccctcgt cgagcgtagc cacctgacga ccggccacgt caaagaggcg
     1501 cagcgatacg aatgcctcgc gcggcacttc ataagcaatg cgcgtgacgc cggtcatcgg
     1561 gttcgggtaa ccgggttcga ggcgcatcgg cacgggaacg accggagttt ccggcaggcc
     1621 ggtgacgagc gtgtccacga cctcgaactg gcgcatcatc tcatgatctt cgtgctcgag
     1681 cacgtggcag tgatagggat atcgccccgt gtagtcgtcg aagcgggcaa tcacgcgcac
     1741 ggcctcgccc ggcagcaccg gcaccgtgtc cttccagcca cccagcgcgg agtccggcgt
     1801 gaccggcgtg ccgaagacca gggtatcggc cacgacatcg aacggtgccc ggtccagcac
     1861 ctggaagtgc acgaggtgga ggtgcatcgg gtgcaccacg ccggagcggt tgatgaatcg
     1921 ccagacttcg gtgtcgccca ggcgcggcag ctcggtgatg tcgtcccagt gcagcccgtt
     1981 gatggtccac atctggccgg tgcagtcatc cgccatcttc tcgagagaga aatcgcgggc
     2041 catgtccgcc gttgccggat cgagagccga cggcgggcgc aggatgcccg gcggtgtcca
     2101 ggcgtatccc gcttccggcc ggaccacgaa cttcatgatc gcggggaggg ccggatgcat
     2161 cggatcgccg atcgggtatg gggcaggtgc cgaattggtc aggatgatcc cggttccggc
     2221 cgccaccccg ctgaagtcga cgatggtgtc gatccgttcg ccgggggtaa tgaggaggct
     2281 gtcccgtcgg accggtcgtt cgaggagtcc ggcgtcgccc ccgatgaccg tgaagggaag
     2341 cccgttcgac aaccggagga tgtacgtgcg cgagttcgac ccgttcagga gacgcaggcg
     2401 gtacgcgcct cgacggactt ccgcgaaggg ccagaccgtc ccgttgacca gcgcgacgtc
     2461 gccgaagaag tgttcctccc agattgccgg ataggagagc gagccgtcgg catgaaagcg
     2521 ccggtcctgg atgatgatcg gcaggtcgta cgcgccacct ggcaggctga gagccccctc
     2581 gaccgggtcg gtgacgacgt accctcccgc cagccccatc atgacgttga gccgcgtcgt
     2641 gccgagcgcg tgatcgtggt accagatgag cgcggccgcc tggttgttgg gataggtgta
     2701 cgtccgcgac tcgccgggca ggaaagtact gtcgggatgc ccgtccacgc tttgcggcac
     2761 gtgacccccg tgaaggtgaa cgacgacgcg ggccccggtg cccgcggtgt caggaccatg
     2821 gagacactgg tcgaccggca ggtagtgggt ggttcgcaag gcgccggtgg aatcgcgcag
     2881 gtcgttgatc cagttcaccg tgaccgggag tccacgggtg gccacgatcg tcggtcccgg
     2941 gtaatgcccc tcgtagcccc agacggtggt ggggggaagg tcgcgatgca gcttcttccg
     3001 gaattccacc atccgcatgt cgtaggtggc gatccccccc accgttcccg aaatcggcac
     3061 cgcaaccggc gggaccgtca gcgcatcgac gaagggcgtg aggacaaccg ggcacccctc
     3121 cgggtggcag gccgtcccct caccctgcca gtggccaccc agggactggc atgccgtttc
     3181 ggtttgctcg tcacacgtgc cgtcatcgag acagcaggca cccgtggggt cgggcggagg
     3241 gacggtgtac gtgatggtga ggcgcggccg gtatgacgtg tccgcggcgt actgccggct
     3301 cgcgtagcgt ttttcggtgt tgccggcgtt ctcgacgccg accagcaccc agccgaagtt
     3361 ggtcgcaacg ccgtcgagcc aggcctgcac gtccgtgacc attcccggac ccgaccaggc
     3421 gtaggggttc tgggcggcaa cgctcgccgt cgcgctgggt gtcccctggt actgcccccc
     3481 gggagcactc cagaggaggg tgttgtagaa gcgatggagc caggttgcgt cgccactcgt
     3541 cgcggccgca ccggcgccct cgcttcccgc cgcattcgag gtcccttccc cccaatccgc
     3601 caggacacga tgcacgttga cgtccgtcgc gccgccgctc gtcgtggatc gaagggtcag
     3661 cgtggcggaa ctcaccacgg ctccggccgg gaggccggcc atcgggaaac gaacgagtgc
     3721 gcgccgacgg agcgcctgat tgttgcgacc ggtgaaaatg
//
LOCUS       DASVOD010000252         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_642506, whole genome shotgun sequence.
ACCESSION   DASVOD010000252 DASVOD010000000
VERSION     DASVOD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642506"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF720_05435"
     CDS             <1..411
                     /locus_tag="VF720_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878829.1"
                     /translation="LSPRGSDFDVQPLQTRRVSAWLKANTPADSTVLVWGFEPAVNFL
                     SERRSATRYLYDWYLTSRAVEPGRQARYWETFWREVEGSPPSAVVVVHDDMNPVEGKD
                     SATQLASSPQLAEWLAANYTSEIRIGDFEVYRRP"
     gene            527..853
                     /locus_tag="VF720_05440"
     CDS             527..853
                     /locus_tag="VF720_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX7878830.1"
                     /translation="MATTPDKTTGSSEPKPGSKKGAAQNIPARLARVEGQVRGIAKMV
                     DEKRYCIDILTQVAAVHEALRAVSREIVQNHMTSCVARAMRSGDATEAERVSREMSEL
                     LDRFTR"
     gene            864..1079
                     /locus_tag="VF720_05445"
     CDS             864..1079
                     /locus_tag="VF720_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEX7878831.1"
                     /translation="MTTSKLTVNGMTCGGCVASVTRVLKATPGVSECEVDLAGKSATV
                     TFDPTVTSVDVLVERIDNAGFEATPAV"
     gene            1128..>2639
                     /locus_tag="VF720_05450"
     CDS             1128..>2639
                     /locus_tag="VF720_05450"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7878832.1"
                     /translation="MKRNTTLDLTGMYCAACAGRIEKVLTKTPGVESAYVNFATERAT
                     VTHDDDAAAVDKLIARVGDAGYGASLRLPAASRRGRAMDAEEAARRAKHADLARRVAV
                     SGALAVVMALLSMPLMSAMGPSGHDLWHRLMAPIDAFGRGVAPWLYAIPADTLRWLSF
                     ALTLPIVLWAGRDIYRAAWNGARHRTADMNTLIAVGTGAAFAQSLAATIGARAYERAG
                     LPTDVYWESVAWIITLVLTGRWLESRARGRAAGAVRGLLELTPHVAHVVRDDREVDLD
                     VEDVAVGDRVVLRPGERVPVDGVVESGASTMDESMLSGESLPVEKSAGDAVHAGTLNG
                     TGSLTITATRVGQDTTVGRIASFVEAAQGSRAPIQRMADQVAAVFVPIVMVIALLAAV
                     AWLIVGPEPRLWYAVSAAVTVLIIACPCALGLATPTAVMVGTGRAAELGILIKNAAAL
                     EVAGRVNVVLFDKTGTLTEGRPRVTTVVAEDERGLLRLAASAESRSEHPLGRAV"
BASE COUNT          436 a          821 c          960 g          422 t
ORIGIN      
        1 ctctcgccac ggggctcgga cttcgatgtg caaccgctcc aaactcgacg cgtgtcggcc
       61 tggctgaagg ccaacacgcc ggccgattcc actgttctcg tgtggggatt cgagccggcc
      121 gtcaacttcc tgtccgaacg acggagcgca acccgctacc tgtacgactg gtatctcacc
      181 tcgcgcgcgg ttgagccggg acggcaggca cgctattggg aaacattctg gcgggaggtc
      241 gagggctcgc caccgtcggc cgtcgtcgtc gttcacgacg acatgaaccc cgttgagggg
      301 aaggactcgg ccacgcaact ggcgtcgagt ccgcagctgg ccgagtggct ggcggccaac
      361 tacacgagcg aaatcaggat cggggatttc gaagtttatc ggcgaccgtg agcgccacgc
      421 tctttccctg aatcctaccc cccggggtat actccaagaa ttggcccgtc gcggccccat
      481 ctaataggtt gcaactcggg tgccggacgg ccggaaaaga ggacaaatgg cgacaactcc
      541 cgacaagaca acaggttcga gcgaacccaa gccaggatcg aagaaggggg ccgcccagaa
      601 catcccggcc cgcctggcca gggtcgaagg acaggtccgc ggcatcgcga agatggtcga
      661 cgagaagcgc tactgcatcg acatcctgac gcaggtggcc gccgtgcacg aggcgctccg
      721 ggcggtgagt cgggagatcg tgcagaacca catgacgagt tgcgtggccc gggcgatgcg
      781 ttcgggcgat gcgacggaag ccgagcgggt gagccgcgag atgagcgaac tgctcgaccg
      841 gttcacgcga taagggagat tcgatgacga cgagcaagct gaccgtgaac gggatgacct
      901 gcggtggttg cgtggcgagt gtgacgcgtg ttctcaaggc cacacccggc gtgagcgagt
      961 gcgaagtgga cctggccggc aagtcggcga ccgtgacctt cgatccgacc gtgacatcgg
     1021 tggatgtcct ggtcgagcgg atcgacaacg ccggcttcga ggctaccccc gcggtttgac
     1081 tgccgctgca atgaggtctg tctgagcttc cacgaagtca accaatcatg aaacgaaaca
     1141 ccacgctcga cctgaccggc atgtactgtg ccgcctgcgc cgggcgaatc gaaaaggtgc
     1201 tgacgaagac gcctggcgtg gagtcggcct acgtgaactt tgccaccgag cgggccaccg
     1261 tcacgcatga cgacgacgcg gccgctgtgg acaaactgat cgcccgcgtg ggtgatgccg
     1321 gttatggggc atcactgcga ttgccggcgg cctcgcgacg tggccgggcc atggatgccg
     1381 aagaggcggc caggcgggca aagcacgccg acctggcgcg gcgggtggcg gtgtcgggtg
     1441 cgctggccgt cgtcatggcg ctgttgtcca tgcccctcat gtcggcgatg ggtccttccg
     1501 ggcacgacct gtggcaccgg ctgatggcgc cgatcgacgc gttcggccga ggcgtggcgc
     1561 cgtggctcta cgccattccg gccgacacgc tccgctggct ctccttcgcg ctcacgcttc
     1621 ccatcgtgct ctgggccggc cgcgacatct accgggcggc gtggaacggt gcgcgccatc
     1681 ggacggccga catgaacacc ctgatcgcgg tcggaacggg ggctgccttc gcccagagcc
     1741 tggcagccac gatcggggcg agggcgtacg agcgggcggg gttgcccacc gacgtctact
     1801 gggaatcggt ggcgtggatc atcaccctcg tgctcacggg gcgctggctc gaatcgcggg
     1861 cccgcgggcg tgcggccggc gcggtgcggg gattgctgga attgacacct catgtcgcgc
     1921 acgtggtgcg cgacgaccgg gaggtcgatc tcgatgtcga ggacgtcgct gtgggcgacc
     1981 gggtcgtgct ccgacccggg gagcgggtgc cggtggatgg cgtggtggag tcgggggcgt
     2041 ccacgatgga cgagtcgatg ctcagtggtg agtcgctgcc ggtggagaaa tccgcggggg
     2101 acgcggtgca cgccggcacg ttgaacggca cgggatcgct caccatcacg gccacgcggg
     2161 tcggccagga cacgacggtg ggtcgcatcg cctcgttcgt cgaggcggcg caggggtcgc
     2221 gcgcgcccat ccagcgcatg gccgaccagg tggcggccgt cttcgtgccg atcgtcatgg
     2281 tcatcgcgct gctggccgcg gtggcctggc tgatcgtcgg gcccgaaccc cgcctgtggt
     2341 acgccgtgag cgcggcggtc accgtgctca tcatcgcctg cccgtgtgcg ctcggactcg
     2401 ccacgccgac ggccgtcatg gtggggacgg ggcgcgcggc cgaactcggc atcctcatca
     2461 agaacgcggc ggcgctcgag gtggcggggc gggtgaacgt ggtgttgttc gacaagaccg
     2521 gcacgcttac cgaaggaagg ccgcgcgtga cgacggtcgt ggccgaggat gagcgtgggc
     2581 tgcttcgatt ggcagcctcg gccgagagtc ggtccgaaca tccgctgggg cgggcggtc
//
LOCUS       DASVOD010000253         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_329477, whole genome shotgun sequence.
ACCESSION   DASVOD010000253 DASVOD010000000
VERSION     DASVOD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329477"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF720_05455"
     CDS             complement(<1..518)
                     /locus_tag="VF720_05455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7878833.1"
                     /translation="MNCGIEALGATDIYLIDQIMKGRYAAPASILDAGAGHGRNLSWF
                     AKQGLVIHGTDRDAGAVEQLRRNYPGVPAENFQVALVDELPFSDARFDHVICCAVLHF
                     AESESHFRKMFGELVRVLKPGGSLFARVATDVGLADRMIPLGHGRFTMPDGSDRFLLT
                     RSLLASLLDVHRL"
     gene            680..>2499
                     /locus_tag="VF720_05460"
     CDS             680..>2499
                     /locus_tag="VF720_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7878834.1"
                     /translation="MKRLHVVICLLVLCASLPATPTFGQTCTIGGHNETSENFAPGGW
                     GITPPTLGSGPAGLPVPHLVISEVAPRGFGNGVASDSSEYVEIYNPTARPVHLDDKYL
                     SDDIGYYRIVNGPYPAANNTDWAGRFPAGLTLDPGRTLIVCVTKAGFAASGASAPLAQ
                     YVLEMRDSNANPTDDMTILTTVTSIPLIGGMMTNPSATNGEWIVLYCWNGVSDLVCDL
                     DYASWGATSATNPKMNKSGISLDGPDAGAVPSAYNADTAPAGQSNLGAGTALTKPNTY
                     QRVGGGEAAEVNVGGNGCLGRVQPTVINWLPVAGTDHIRFHVRWTNMDEDSPSSQVSG
                     QMLSQDFGVFQPDFGSIGSFNVPPLQPSSFFDVFFEVPLSSLPPAPQKILPGGGPIAP
                     PGLLADSGPATLSDCPPDTNWAGNVDIWWLGPGYDGMVNKHYADLLTCAGGPPSYIHF
                     RGSNCPSPMPWTISGLAPGFSATLVNEDFTPAPNPVPVGWTGWICVSAAAGVPSGTSS
                     CFGVTFTCDDESSTIDICSTACDWSNPQPPVLSVIDWVNVGSLVKFHLGWHNENTTTA
                     SLPVSGDLRSQPFGVFQPIFGHIGHFDVPPLPPNSFFDVFT"
BASE COUNT          430 a          855 c          794 g          420 t
ORIGIN      
        1 aggcggtgaa cgtccagcag gctcgccagc agggagcgcg tgagcaggaa ccggtcgctg
       61 ccgtcgggca tcgtgaaccg gccgtgaccg agcgggatca ttcgatcggc cagccccaca
      121 tcggtggcca cgcgcgcaaa gagtgagccg cccggcttca gcacccggac cagctcgccg
      181 aacattttcc gaaagtggga ttcgctttcg gcaaagtgca ggaccgcgca acagatgacg
      241 tggtcgaagc gggcgtcgga aaacggcagc tcatcgacca gcgccacctg gaagttttcc
      301 gccggcacgc cgggataatt ccgccgcaac tgctccacgg cccccgcatc ccggtcggtg
      361 ccgtggatta ctaatccctg cttcgcgaac caggacaggt tccttccgtg gcctgcgccc
      421 gcatcgagga tggaggcggg cgcggcgtac cgccccttca tgatctggtc gatcaggtag
      481 atgtcggtgg cgccgagcgc ctcgatgccg caattcatgg ctggatcgtg gacccggctg
      541 gaggtggtgt aaaccccttc ggcaccggcg tttgaccaat cttgtatcaa tgcccccccg
      601 cggggagcat aatcgccccc tgcccgaaac ccctctcctc gaaattgcgt tccgtccaca
      661 acgactattg ggggatgcca tgaaacgcct gcatgtcgtc atctgcctcc tggttctgtg
      721 cgcctctctg ccggccacgc cgacattcgg ccagacctgc acgatcggcg ggcacaacga
      781 aaccagcgag aacttcgcgc ccgggggctg gggcatcacg ccccccacgc tcggaagcgg
      841 gccggccgga ctgccggtgc cgcacctcgt gatcagtgaa gtggcgccgc gcggtttcgg
      901 gaatggcgtg gcctcggact cgagcgagta tgtcgagatc tacaacccga ccgcgcgacc
      961 ggtgcacctc gacgacaagt acctgagtga tgacatcggc tattaccgga tcgtgaacgg
     1021 cccttatccc gccgcgaaca acaccgactg ggccggacga ttcccggccg ggttgacgct
     1081 cgacccgggc cgcacgctca tcgtgtgcgt gacgaaggcg ggattcgccg cctccggcgc
     1141 ctccgcgccg ctggcgcagt acgtgctgga gatgcgcgac tcgaacgcga acccgacgga
     1201 cgacatgacg atcctcacca cggtcacgtc gattccgttg atcggcggca tgatgaccaa
     1261 cccgagcgcc acaaacgggg aatggatcgt gttgtactgc tggaacggcg tttcggacct
     1321 ggtatgcgat cttgactatg cctcgtgggg agctaccagc gccacgaatc ccaagatgaa
     1381 caagtccggc atctcgctgg acgggccgga cgccggcgcc gtcccctcgg cctacaacgc
     1441 cgataccgca cccgcgggcc agtcgaacct gggcgccggt acggcgctca ccaagcccaa
     1501 cacctaccag cgcgtgggtg gcggcgaggc ggccgaagtg aacgtgggcg gcaacggctg
     1561 cctggggcgt gtgcagccca ccgtgatcaa ctggctgccg gtggccggca ccgatcacat
     1621 ccgtttccac gtccgctgga cgaacatgga cgaggactca ccgtcctcgc aggtgtcggg
     1681 acagatgctc tcgcaggact tcggggtgtt ccagcccgac ttcggttcga tcggatcctt
     1741 caacgtgccg ccgctgcagc cgtccagctt cttcgacgtc ttcttcgagg tgccgctgtc
     1801 gtcgctgccg ccggcgccgc agaagatcct tccggggggt ggccccatcg cgccgccggg
     1861 gctcctggcc gactcgggcc cggcaacgct gtccgactgc ccacccgaca ccaactgggc
     1921 gggaaacgtc gacatctggt ggcttggccc cggctacgac ggcatggtca acaagcacta
     1981 tgccgacctc ctgacatgcg cgggggggcc gccgtcgtat atccacttcc gcggcagcaa
     2041 ctgcccgagc ccgatgccct ggacgatctc ggggctggcg cccggtttca gcgccactct
     2101 ggtgaacgag gacttcacgc cggcgccgaa cccggtgccg gtgggatgga caggatggat
     2161 ctgcgtgtcc gccgcggccg gggtgccgag cggaacgagt tcctgcttcg gcgtgacgtt
     2221 cacgtgcgat gacgagtcga gcacgatcga catctgctcg acggcctgcg actggtcgaa
     2281 cccccaaccg ccggtgctca gcgtcatcga ctgggtcaac gtggggagcc tcgtgaaatt
     2341 ccaccttggg tggcacaacg aaaacacgac gacggcgtcg ttaccggtca gcggcgacct
     2401 ccgttcgcag ccgttcggcg tcttccagcc gatcttcggc cacatcgggc acttcgacgt
     2461 gccgcctctc ccgccgaaca gcttcttcga tgtcttcac
//
LOCUS       DASVOD010000254         2316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_278433, whole genome shotgun sequence.
ACCESSION   DASVOD010000254 DASVOD010000000
VERSION     DASVOD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278433"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF720_05465"
     CDS             <1..629
                     /locus_tag="VF720_05465"
                     /inference="COORDINATES: protein motif:HMM:NF017024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7878835.1"
                     /translation="ARPDDFRVVDRIDSRDAGGCRARLDAHPDAVVVDFSLAAAVPAL
                     LEIMDGRSNPLVSGTTGLGDTERRGLSNRASKAAVVWAPNMGLGIPLLARLVREAMAL
                     VPDAQVAIVETHHRHKKDRPSGTALYLAEAAGGSVDMHSLRIGEVVGEHRLELAFGEE
                     RLTFTHQALSRDVFADGALAAARFAAGATAGLYDMEAVLAGLAGRSSR"
     gene            626..1789
                     /locus_tag="VF720_05470"
     CDS             626..1789
                     /locus_tag="VF720_05470"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX7878836.1"
                     /translation="MSLTSRRVRELPPYMFAEIDRVKTRLREEGHRLLDFGVGDPDLP
                     TPGFIVDAMVEAARSPKHHRYPSYAGMKSFRQAAADWCRHRHGISVDPDTELLTLIGS
                     KEGIAHFPLGVIDPGDLVLVTDPGYPVYVNTVRFAGGEVALVPLSEEHGWLPDLDAID
                     PALAAKAKLFILNYPNNPIGATADRDFFERLIAWAKKHDVFILHDAAYIEVGYDGWRP
                     PSLLALPGGKEVGIEFHSLSKTFNMTGWRIGFAAGNAEAVGALAKIKTQIDSGVFEPV
                     QVAAIEAYKSGESALGDILAVYQRRRDRMVTALRALGLEAMNPRGAFYIWTRVPAGQT
                     SAAFARRLLEESGVVVMPGTGFGARGEGWFRFSLTVADAGIEEMLAVLPKAIG"
     gene            1805..>2316
                     /locus_tag="VF720_05475"
     CDS             1805..>2316
                     /locus_tag="VF720_05475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7878837.1"
                     /translation="MGDTILRPLASIAEIQAAVDLQRDVWQAEDRDLVPATEIVAARS
                     NGGVVLGAWSGSRLVGFTFSMIGRRSGRVYQYSRMLAVHPEWRGHGLGAALKLAQKEA
                     SLAAGDRWMEWTFDPLEGRNANLNVARLGVRVRHYERDFYGARTSRFDLGIPTDRVRA
                     EWDLEEDLDVT"
BASE COUNT          337 a          804 c          794 g          381 t
ORIGIN      
        1 ccgcgcgccc cgatgacttc cgcgtggtgg accggatcga cagccgcgac gcgggcggct
       61 gccgcgcgcg gctcgacgcc catccggacg cggtggtcgt cgatttctcg ctggccgccg
      121 ccgtcccggc acttctcgag atcatggacg gacgctcaaa tccgctggtg agcggaacca
      181 ccggactcgg cgacaccgag cggcgtggcc tctccaatcg ggcctcgaaa gccgcggtgg
      241 tctgggcgcc caacatgggg ctcggcatcc ccctcctcgc gcgactcgtg cgcgaggcaa
      301 tggcgctggt ccctgacgcg caggtggcca tcgtcgaaac gcatcatcgt cacaagaagg
      361 atcgcccgag cggcacggcc ctctacctgg ctgaagcggc cggcggatcg gtggacatgc
      421 actcgctgcg gatcggcgaa gtggtgggtg agcaccggct ggagctggca ttcggcgagg
      481 aacgcctcac cttcacgcac caggccctct cgcgcgacgt ttttgcggat ggcgcgctgg
      541 cggctgcccg attcgctgcg ggcgcgaccg ccgggctcta cgacatggaa gccgtgctgg
      601 ccggactggc cggaaggagc tcacgatgag cctcacctcg cggcgggtgc gcgagctgcc
      661 cccgtacatg tttgccgaga tcgaccgggt gaagacccgc ctgcgcgagg agggtcaccg
      721 cctgctcgat ttcggcgtgg gcgatcccga cctcccgacg ccgggtttca tcgtcgacgc
      781 catggtcgag gcggcccgct cgccgaaaca ccatcgttac ccgagttacg ccgggatgaa
      841 gtcgttccgc caggcggcgg ccgactggtg ccgtcatcgc cacggcatct cggtcgatcc
      901 ggataccgaa cttctcaccc tcatcggctc caaggagggg atcgcgcact ttccgctcgg
      961 cgtcatcgat ccgggcgatc tcgtgctggt caccgacccc ggctacccgg tgtacgtgaa
     1021 caccgtccgc ttcgccggcg gggaggtggc gctggtgcct ctctccgagg agcatggctg
     1081 gcttcccgac ctggacgcca tcgacccggc gcttgcggca aaggccaagc tcttcatcct
     1141 caactatccg aacaacccga taggcgccac cgccgatcgc gacttcttcg agcggctcat
     1201 cgcgtgggcg aagaagcacg acgtcttcat cctccacgac gcggcctaca tcgaagtggg
     1261 ttacgacggc tggcggccgc ccagcctcct cgcgcttccc ggcggcaagg aggtggggat
     1321 cgagttccac tccctctcca agacgttcaa catgaccggc tggcgcatcg gcttcgcggc
     1381 cggcaatgcc gaggcggtcg gtgccctcgc caagatcaag acgcagatcg actccggcgt
     1441 cttcgagccg gtgcaggtgg cggccatcga ggcctacaag tcgggtgagt cggccctcgg
     1501 cgacatcctg gcggtgtacc agcggcgtcg cgaccggatg gtgacggcgc tccgcgccct
     1561 cggcctcgag gcgatgaacc cgcgcggcgc cttctacatc tggacgcgcg tgccggccgg
     1621 ccagacgtcg gccgcctttg cgcggcgcct cctcgaggag tcgggcgtgg tggtcatgcc
     1681 cggcaccggt ttcggcgcgc gtggcgaggg gtggttccgc ttttcgctca cggtggccga
     1741 tgccgggatc gaggagatgc tggccgtcct cccgaaagcc atcgggtaac cggggcgctc
     1801 gctggtgggc gacacgatcc tccggccgct cgcgtcgatc gccgagatcc aggcggcggt
     1861 ggatctgcaa cgggatgtct ggcaggccga ggaccgcgac ctcgttcccg ccaccgagat
     1921 cgtggccgcc cgttcgaatg gcggcgtcgt cctcggcgcc tggtccggat cccggctggt
     1981 cggcttcacc ttctcgatga tcggccggcg gtccggccgg gtgtaccagt acagccggat
     2041 gctggccgtc catcccgagt ggcgcggcca cgggctcggc gccgcgctca agctggccca
     2101 gaaggaggcc tcgctggcgg ccggcgaccg ctggatggag tggacgttcg atcccctcga
     2161 aggacggaat gccaacctca acgtcgccag gttgggcgtg cgcgttcgcc actacgaacg
     2221 tgatttctac ggcgcccgca ccagccgttt cgacctgggc attccgaccg atcgcgtgcg
     2281 cgcggagtgg gatctcgagg aagatctcga cgtgac
//
LOCUS       DASVOD010000255         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_435057, whole genome shotgun sequence.
ACCESSION   DASVOD010000255 DASVOD010000000
VERSION     DASVOD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435057"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="VF720_05480"
     CDS             complement(<1..1713)
                     /locus_tag="VF720_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878838.1"
                     /translation="MTLLSIAARVAGVLAASLLVAAGIAAAQSQLPDSTAARPAAPPL
                     FDTSGGFNLEAREMLGQQEAVGGLRHILLDVTITQKGLVVKAPRGYYYTGRDLTAMGG
                     GVTMVDSTVTVVADSAAYYRTAQRMEAFGKVTVEDGSFHVEGKHGAWDRAADRLVLDG
                     GVTGGEEGRRFSGDRMEWDRVTDSYAIIGHARYEDPDRNFILTGQRLDWDATGAAVAT
                     GEPSLDWIRDGEPLSVTGERLRFDDVGGELDAAGNVTVKQGRQTVTADSARFLDREGL
                     GWFHGSPRVSDVDGTVTGDSLRVSFEDAKIDQAVMLGNAVLERRPTAPDLAGEESRIT
                     GDSLTVKFVDGEVEGLSALGSPTIAYKPSRADSARGTGRIEGKADTIRITIDTGGLNE
                     VRLAGTASGRYLYRSSAGADSIDKVDYAANRILFKVPERKIELRGQGQTDYGTLTLKA
                     DEIDFDAEREELTAQPKPILIDRSRPEEQQVTGNEVTYDLNSSRGTIYHGRTQYDTGY
                     LFADSLRKVTDTELNAGSGRYTTCDLVGQDQEPHYHFTSRRMKVYLGDKVVAKPVTLY
                     LGRVP"
     gene            complement(1732..>2689)
                     /locus_tag="VF720_05485"
     CDS             complement(1732..>2689)
                     /locus_tag="VF720_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX7878839.1"
                     /translation="TERLAAGVDIGGTGLKVGLVRPSNAERVGALRILSGHATDDPIR
                     VLGAVADTIRELLAAEGLGETGLAAIGVGSAGLVDAKAGIVRTSPNLPSWRDIRVRDE
                     LSARLDPRLAPRVTLINDAAAFLAAEWLAGAAEGYGDVLFVTLGTGVGGGLIMGGRPW
                     RGPTGLGGEIGHMSIDMDGPECPCGNRGCLERFVARGAIERLAVELGLAAEGADNPKR
                     VHERALAGDARASRLFAEIGRRLGAGLAGLANLLEPRLIVVGGGIAEAHDFILPAARA
                     EMELRSMVARQRPLPVVPARFGPEAGVVGAALVAGRGDDRFA"
BASE COUNT          378 a          938 c          931 g          442 t
ORIGIN      
        1 cggcacacgc ccgaggtaga gcgtgaccgg cttggccacg accttgtctc ccaggtacac
       61 cttcatgcgg cggctggtga agtggtagtg cggctcctgg tcctggccca ccaggtcgca
      121 ggtggtgtag cgaccggagc cggcgttcaa ctcggtgtcg gtgacttttc gaaggctgtc
      181 cgcgaagaga tagccggtgt cgtactgggt tcgcccgtgg tagatggtgc cgcgcgacga
      241 attgaggtcg taggtcacct cgtttcccgt gacctgctgt tcctccgggc gcgatcggtc
      301 gatgaggatc ggcttgggct gcgccgtcag ctcctctcgc tccgcatcaa agtcgatctc
      361 gtccgctttc agcgtgagcg ttccgtagtc ggtctgaccc tggccgcgga gctcgatctt
      421 ccgttccggc accttgaaga ggatgcggtt ggccgcgtag tcgaccttgt cgatcgagtc
      481 ggcgcccgcg ctcgatcggt agaggtagcg gccgctggcc gtgccggcca gtcgcacctc
      541 attcagcccg cctgtgtcga tggtgatgcg gatcgtgtcg gccttccctt cgatgcggcc
      601 ggtgccgcgg gccgaatcgg ccctcgacgg cttgtaggcg atggtggggc tgcccagcgc
      661 cgacaagcct tccacttcgc catccacgaa tttcaccgtg aggctgtcgc cggtgatgcg
      721 gctctcctcc cccgcgaggt ccggcgcggt cgggcggcgt tcgaggaccg cattcccgag
      781 catcaccgcc tggtcgatct tcgcgtcctc gaacgacacg cggagcgaat caccggtgac
      841 ggtgccgtcg acatcggaaa ccctgggcga gccgtggaac cagccaagtc cctccctgtc
      901 caggaaccgg gcgctgtcgg cggtgaccgt ctggcgtccc tgtttcaccg tgacgttgcc
      961 cgccgcgtcg agctctcccc cgacatcatc gaaacggagg cgctcgcccg tcaccgagag
     1021 gggctccccg tcgcggatcc agtcgaggga cggttcgccg gtggccacgg ccgcgccggt
     1081 ggcatcccag tcgagtcgct gtccggtcag gatgaagttg cggtcggggt cctcgtagcg
     1141 ggcgtgcccg atgatggcgt aactgtcggt cacccggtcc cactccatcc ggtcgccgct
     1201 gaagcggcgc ccctcttccc cgccggtgac gccgccgtcc agcaccagcc ggtcggcggc
     1261 gcgatcccag gcgccgtgtt tcccttccac gtggaacgag ccgtcctcga cggtgacttt
     1321 cccgaacgct tccatcctct gtgccgtgcg atagtaggcg gccgagtcgg cgaccaccgt
     1381 cacggtgctg tccaccatcg tcacgccgcc gcccatcgcc gtgagatcgc gccccgtgta
     1441 gtagtagccg cgcggcgctt tgaccacgag ccctttctgc gtgatcgtga cgtcgagcag
     1501 gatgtgccgg agaccgccga ctgcctcctg ctggccgagc atctcgcggg cttccaggtt
     1561 gaagccgccc gaggtgtcga aaaggggggg cgccgcgggg cgcgccgccg tggaatcggg
     1621 cagctgactc tgcgcggccg caatgccggc ggcaacaaga agagaggccg cgagtacccc
     1681 tgcgacccgg gcggcgatgg acaggagagt catcggtgag gcccgggtgg atcaggcgaa
     1741 gcggtcgtca ccgcgtcccg ccaccagggc ggcgccgacc acgcccgcct ccggtccgaa
     1801 ccgagccggt acgaccggga gggggcgctg gcgggccacc atcgagcgga gttccatttc
     1861 ggcacgggcg gccggcagga tgaagtcgtg ggcctccgcg atgccgcctc ccaccacgat
     1921 gagccgaggc tcgaggaggt tggccaatcc cgcgaggccc gccccgaggc ggcggccgat
     1981 ctcggcgaag agacggctcg cgcgcgcatc gccggcgagg gctcgttcgt ggacccgttt
     2041 cggattgtcg gctccctccg cggcaagtcc cagctcgaca gccagacgtt cgatcgcgcc
     2101 ccgggccacg aaccgttcga ggcaaccccg gttcccgcac ggacattccg gtccgtccat
     2161 gtcgatgctc atgtggccga tttcgccccc gaggccggtg ggaccgcgcc acgggcgtcc
     2221 gcccatgatg agcccgccac cgacaccggt gccgagcgtg acgaagagga cgtcgccgta
     2281 tccctcggcc gcgccggcca gccactcggc ggccaggaac gccgcggcgt cgttgatgag
     2341 ggtcacgcgt ggtgcgaggc gcggatcgag gcgggccgac agctcgtccc gaaccctgat
     2401 atcgcgccag ctcggcaggt tgggcgaagt gcggacgatg cccgctttcg catcgaccag
     2461 ccccgccgat ccgacgccga tcgcggcaag tccggtttca ccgagccctt ccgcggcgag
     2521 gagctcgcgg atcgtgtcgg caaccgcccc gaggacccgg atgggatcgt ccgtggcgtg
     2581 accggagagg atgcggagcg ccccgaccct ctcggcgttg gacggacgga cgagcccgac
     2641 cttgaggccg gtgccgccga tgtcgacccc ggcggcgagc cgctcggtc
//
LOCUS       DASVOD010000256         3456 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1009901, whole genome shotgun sequence.
ACCESSION   DASVOD010000256 DASVOD010000000
VERSION     DASVOD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009901"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VF720_05490"
     CDS             <1..361
                     /locus_tag="VF720_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878840.1"
                     /translation="IIGILAAIAIPNFISMQDRAREGAVKSNMHTLQTGVEDFAILNS
                     GRYPDGGAADGATVESLLNGTVVPQNPFTKVPTTVTWGGPAAAQGDLGYEVPGTNQYD
                     ISGFGAAALLGLTLSNH"
     gene            571..1680
                     /locus_tag="VF720_05495"
     CDS             571..1680
                     /locus_tag="VF720_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878841.1"
                     /translation="MSDLLHLERRRTARSGLGSDATESPVVITGLRRSYRGKWGRKVE
                     ALKGVSLEAPAGRVTGLLGPNGSGKTTTIKILLGMLRASSGTVKLFGRSMEDPATRAR
                     LGFLTEQPYFYDYLTARELLDLCGVLCGIPSAERKRRTDRWLERMGLAASADLRLRKF
                     SKGMLQRLGLAQALIHEPELAILDEPMSGLDPLGRSLVRDVILDLKRNGTTVLFSSHV
                     LPDVETLCDRVTVLAGGSVVLDGTVTDLVADADPEFEIVAAGASPALRMKLTSRGTEM
                     AVSASRLLIGAKDRAAMNELVAELIADGAEIATVTPRRSPLEQRFLAAVRGAGSSAGM
                     HDGGSNSLRPASAEEILSHATAHSSSDTSRTGTDA"
     gene            1677..2444
                     /locus_tag="VF720_05500"
     CDS             1677..2444
                     /locus_tag="VF720_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878842.1"
                     /translation="MTRILAIAANTWREAIRDRIYLALLAFAALLILASQVLSPLALG
                     EGTRITRDFGLSGLVLVSLLVAIFVGTGLVQKEIERKTVMTMLSKPLGRGEFVIGKYL
                     GLLATLAVIFVSMTVLLMMALLWRGDGLNTPVLAAAVLSLGEIVIITGVAIFFSTCTS
                     PVLSGLMTLAVFVAGHFTADLATFSEQAASPVLAGISGALHWIFPNLELFNVRNAAAH
                     GVMPESVRFALGAGYALLYTAALLIAAVVVFRRREFR"
     gene            2461..3321
                     /locus_tag="VF720_05505"
     CDS             2461..3321
                     /locus_tag="VF720_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878843.1"
                     /translation="MSTPDRFSSGATRDGFLLLLLVATLVSASALAGLESRVCVRDRA
                     AEELMYYPKGPMIEKASLGHTASAADMAWLRAVQYYGEHKRSDRQFGMMNHIVTILTD
                     LDPNFVNAYIFGGMVTAQEGRDVEAAIALMEKGLKHNPDDWQMNFETGFLYYTQARDY
                     DRAATLFRRAAELPGASDATIRFAAFVSKRAGDPRASLFLWQEFYNRSTNDELKERAL
                     KAIHRLEAELGRPLSVPGAPSDNTGSTPGAYPSSSPGSNPSSSPGINPSSSHGSNPSS
                     NPSSDPGAAH"
     gene            3318..>3456
                     /locus_tag="VF720_05510"
     CDS             3318..>3456
                     /locus_tag="VF720_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX7878844.1"
                     /translation="MSPLLIVVGLFGALVGSFLNVVIHRLPIHESIVFPPSRCPKCGA
                     GI"
BASE COUNT          579 a         1222 c         1074 g          581 t
ORIGIN      
        1 catcatcggg atcctggcgg ccatcgcgat tcccaacttc atctcgatgc aggaccgggc
       61 ccgcgaaggg gccgtgaaga gcaacatgca cacgctccag accggtgtcg aggacttcgc
      121 gatcctcaac agtggccgct acccggatgg aggcgccgct gacggcgcca ccgtcgagtc
      181 gctcctcaac ggcaccgtcg tcccgcagaa tcccttcacc aaggtgccga ccacggtcac
      241 ctggggcggc cccgccgccg cgcagggcga cctcgggtac gaagttccgg gcacgaacca
      301 gtacgatatc tcaggattcg gcgcggcggc cctcctcggg ctcactctgt cgaaccacta
      361 gtccggttac tagaacgggg cgccgctggc gcctcgcacg gacaggcagc atccgcagca
      421 gcagcccccg caccgcccac aacgtcaggc tccccaccgc ccaccgcccg gaccatcaag
      481 ctccgcaccg cccacactcg ccccagaccg cccgatccgg gtctaaaaac cctaaggcgc
      541 ggacccacgt cggccgataa cagccacatc atgagtgacc tgcttcacct cgaacgccgc
      601 cggaccgcac ggagcggcct aggcagcgat gccaccgagt cgccggtcgt catcaccggg
      661 ctccgtcgtt cctaccgtgg caagtggggg aggaaggtcg aggcgctgaa gggggtgtcg
      721 ctcgaggcac cggccgggcg ggtaaccggc ctgcttggac cgaacgggtc cggcaagacc
      781 accaccatca agatcctgct cgggatgctg cgcgcgagct cgggcaccgt gaaactcttc
      841 ggccgctcga tggaggatcc cgccacccgc gcgcggctcg gattcctcac cgagcaaccc
      901 tacttctacg actacctcac ggcacgcgag ctgctggacc tgtgcggcgt cctttgcgga
      961 atcccgtcgg ccgagcggaa gcgacgtacc gaccgctggc tcgaacggat gggactcgcg
     1021 gcttccgccg acctcaggct tcgcaagttc tccaagggaa tgctccagcg cctcggcctg
     1081 gcgcaggccc tgatccacga accggaactc gccatcctcg acgagccgat gtcgggactc
     1141 gatccgctcg gccggagcct ggtgcgcgat gtcatcctgg acctgaaacg aaacggcacg
     1201 acggtcctct tctctagcca cgtgttgccg gacgtcgaga cgctctgtga tcgcgtgacc
     1261 gtgctggccg gcggatcggt ggtgctcgat ggcactgtca ccgacctcgt ggccgacgcg
     1321 gatcccgagt tcgagatcgt ggcggcgggc gcctcgccgg cgctgcggat gaagttgact
     1381 tcgcggggaa ccgaaatggc tgtctccgcc agccggcttc tcatcggagc gaaggatcgg
     1441 gcggcgatga atgagctcgt ggctgaactg atcgccgatg gggcggagat cgccacggtc
     1501 accccgcgcc ggagccccct cgaacagcga ttcctggcag ccgtccgcgg cgccgggagc
     1561 agcgccggaa tgcacgatgg aggatcgaac agcctccgtc cggcatcggc cgaggaaatc
     1621 ctgagccacg ccacagcgca ctcgagctcc gacacgtccc gaaccggtac cgacgcatga
     1681 ctcggatcct tgccatcgcc gccaacacgt ggcgggaagc catccgcgat cggatctacc
     1741 tcgcgttgct cgccttcgcg gcgctcctga tcctcgcctc gcaggtcctt tcgccgctgg
     1801 cgctgggaga gggaacccgc attacccgcg atttcggcct ctcgggcctc gtactggtga
     1861 gcctgctggt agcgatcttc gtcggcacgg gtctcgtcca gaaggaaatc gagcgcaaga
     1921 cggtgatgac gatgctgtcg aagcccctgg ggcgcgggga attcgtgatc ggcaagtatc
     1981 tcggcctgct cgccaccctg gcggtaatct tcgtatcgat gaccgtcctg ctgatgatgg
     2041 cgctgctctg gcgcggcgac ggcctcaaca cgccggttct ggccgcggcg gtcctgtcgc
     2101 tcggtgagat cgtgatcatc acgggcgtgg caatcttctt ctcgacctgc acctcgccgg
     2161 tgctttccgg gctcatgacg ctggcggtgt tcgtggcggg ccacttcacc gccgatctcg
     2221 ccacattctc cgagcaggcg gcttcaccgg tcctggccgg gatctcgggc gcgctccact
     2281 ggatctttcc caatctcgaa ctcttcaacg tgagaaacgc ggcggcccac ggcgtcatgc
     2341 cggagtcggt gcgattcgcg ctgggcgccg gttacgcgtt gctctacacg gcggccctcc
     2401 tcatcgccgc cgttgtcgtc ttccgccgcc gggaattccg ctagtttcgc gccggatcgc
     2461 atgagcaccc ccgatcgatt ctcgtccgga gccacccgcg acggcttcct cctgctcctc
     2521 ctggtcgcta cgctggtctc cgcgtccgcc ttggcgggcc tggagtcgcg tgtgtgcgtt
     2581 cgcgatcggg cggccgagga actgatgtac tacccgaagg ggcccatgat cgagaaagcc
     2641 tcgctcggcc acacggcgtc agcggccgac atggcctggc tgcgcgcggt ccagtattat
     2701 ggcgagcaca agcggagcga tcgccagttc gggatgatga atcacatcgt gaccattctg
     2761 accgacctcg atccgaactt cgtgaatgct tatattttcg gcggaatggt caccgcgcag
     2821 gaagggcgtg acgtggaggc ggccatcgcg ctgatggaga aggggctgaa gcacaatccg
     2881 gatgactggc agatgaattt cgaaactggt tttctgtatt acacgcaggc cagggactat
     2941 gaccgggcgg ccacgctctt ccgtcgcgcg gccgagcttc ccggcgccag cgatgccacc
     3001 atccggttcg ccgccttcgt ctcgaagcgg gccggggatc cccgtgcctc gctgttcctc
     3061 tggcaggagt tctacaaccg atcgaccaac gacgaattga aggagcgcgc cttgaaggcg
     3121 atccatcgcc tcgaggccga actcggccgg ccgctctcgg ttcccggggc acccagcgac
     3181 aacaccggca gcacccccgg cgcatacccg agcagcagcc ccggcagtaa cccgagcagc
     3241 agccccggca tcaaccccag cagcagccac ggcagtaacc ccagcagcaa ccccagcagc
     3301 gaccccgggg ccgcccactg agtccgcttc tcatcgtcgt cgggctcttc ggcgccctgg
     3361 tcggcagctt cctcaacgtg gtcatccatc gcctgccgat ccacgaatcg atcgtatttc
     3421 cccccagccg ctgcccgaag tgcggcgccg ggatcc
//
LOCUS       DASVOD010000257         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_538371, whole genome shotgun sequence.
ACCESSION   DASVOD010000257 DASVOD010000000
VERSION     DASVOD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538371"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF720_05515"
     CDS             complement(<1..849)
                     /locus_tag="VF720_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878845.1"
                     /translation="MTDNYQIRSGGLTAVLLLAAVAPAFAVEGSVGGWATVSHHEGKT
                     GYRLNDMNRNDNAFSGLRINPEVQIRLDGGLQLNAEYLIDAGASTGLSNTFLRLWGQW
                     NEVGNQPWLNLKAGSLPLVFGTWGERANSTANPVIGNPLISGYHTSLSMQFVPTNGDS
                     LLNRRGRGQFGINYGTGTGFKGMVMVYEPCWDVGLEAYGARGVLEYAAAVTQGTPSAA
                     VLFGNETNDEPGFVGRLGLSNLPGPLFGARLGMSYATGAFYSDAVVMPNATLRNEDYD
                     QIVWGYD"
     gene            914..>1719
                     /locus_tag="VF720_05520"
     CDS             914..>1719
                     /locus_tag="VF720_05520"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7878846.1"
                     /translation="MTPLDAVLPPLDPALVADLAVLAGASGVITQSASLTTFECDGLT
                     LERSAPQVVVYPRSTAEAAAVLVRLARDKVPFVPRGAGTGLAGGTIALAAPVVVCTSR
                     MKKVLELDLDNRRARVEAGVVNLEISRVVAGAGFHYAPDPSSQQACTIGGNVGTNSGG
                     PHTLKYGVTVDHVLDVTLVTPDGAVHRLDVGPDSGSGFDLAGLAVGHEGTFGLVTEIT
                     VRLTRKPRAVRTLLGIFDTVADATRTVSAVIASGIVPAAVEMMDNPILAAL"
BASE COUNT          270 a          580 c          589 g          280 t
ORIGIN      
        1 gtcgtaaccc caaacgatct ggtcgtagtc ctcgttgcgg agggtggcgt tgggcatcac
       61 gaccgcgtcg gaatagaagg cgccggtggc gtaggacatg ccgagccgcg cgccgaagag
      121 cggcccggga aggttcgaca ggccgaggcg ccccacgaag ccgggctcgt cgttggtttc
      181 attgccgaag agcaccgccg cggacggcgt gccctgcgtg acggccgcgg cgtactccag
      241 cacgccccgg gcgccgtagg cctcgaggcc cacgtcccag cacggctcgt acaccatcac
      301 cattcccttg aagccggtgc cggtgccgta gttgatgccg aactgccccc gaccgcgccg
      361 gttcagcagc gaatcgccgt tggtcggcac gaattgcatc gacagcgagg tgtgatagcc
      421 gctgatcagc ggattgccga tgacgggatt ggcggtggag ttggcgcgct cgccccaggt
      481 accgaagacg agcggcaggc tgccggcctt gaggttgagc cacggctggt tgccgacttc
      541 gttccactgg ccccacaggc gcaggaacgt gttcgacagg cccgtggagg cgccggcatc
      601 gatcaggtat tcggcgttga gctggagacc gccgtccagc cggatctgca cctccggatt
      661 gatccggaga ccgctgaagg cgttgtcgtt ccgattcatg tcgttcagcc gatagccggt
      721 cttcccctca tgatgcgata ccgtggccca gccgccgacg gaaccttcga ccgcgaacgc
      781 cggcgcgacg gctgccagga gcagcacggc ggtcagccca cccgatcgaa tctgataatt
      841 gtccgtcacc gtcgtatcct ccggtccatt ggggaatatc gaccagtcgg gcaaaagctt
      901 tagtatgagg aggatgacgc ccctcgacgc cgtcctgcct cccctcgacc ccgccctggt
      961 ggcggacctg gccgtccttg ccggagcgtc gggtgttatc acgcaatcgg cttccctgac
     1021 gacgtttgag tgcgacggcc tcaccctcga acgctcggcc ccgcaggtgg tggtgtaccc
     1081 ccggtccacc gccgaggccg cggccgtcct cgttcggctc gcgagggaca aagtgccgtt
     1141 cgttccgcgc ggggcgggca cgggcctggc ggggggcacc atcgccctgg cggcgccggt
     1201 ggtggtgtgc acgtcccgca tgaagaaagt cctggagctg gacctcgaca accggcgcgc
     1261 gcgcgtggag gccggcgtcg tgaacctgga aatcagtcgg gtggtggccg gggcggggtt
     1321 tcactacgcc ccggacccat cgagccagca ggcctgcacc atcgggggca acgtcggcac
     1381 gaattcgggc gggccgcaca cgctgaagta cggcgtgacg gtggaccacg tgctggacgt
     1441 cacgcttgtc acgcccgatg gcgcggtgca ccggctcgac gtgggccctg attcgggatc
     1501 agggttcgac ctggccggcc tggccgtggg acacgaagga acgttcggcc tcgtcaccga
     1561 gatcacggtg cggctcaccc ggaagccccg ggccgtccgc acgctcctcg gcatcttcga
     1621 caccgtggcc gacgccacgc gcaccgtcag cgccgtcatt gcctccggca tcgtgcccgc
     1681 cgcggtcgag atgatggaca acccgatact ggccgcgct
//
LOCUS       DASVOD010000258         5128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_174893, whole genome shotgun sequence.
ACCESSION   DASVOD010000258 DASVOD010000000
VERSION     DASVOD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174893"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2549)
                     /gene="ileS"
                     /locus_tag="VF720_05525"
     CDS             complement(<1..2549)
                     /gene="ileS"
                     /locus_tag="VF720_05525"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX7878847.1"
                     /translation="MNRFPEFSRSVLLADEEAATLRFWQEERVFERQLEMRRDAPRFV
                     FYEGPPTANGRPGIHHVLGRTIKDLVCRYMAMKGHLVERKSGWDTHGLPVELEVEKKL
                     GIQGKEQIEAYGLEKFNKLCKESVFTYKDEWERMTARIGFWLDMDHPYVTLDNDYIES
                     VWWCLSRFWEKGLVYEGFKILPYCPRCETPLSSHEVSQGYADTEDPSVYVKFRSADDP
                     NVSYLAWTTTPWTLISNVALAVNPDEPYVRVRLKPRVGKDGVEKVDTGHAAGTELILA
                     RARLSALYGPYEIAEEFPGSTLHEKRYEPLFPFFAKEARAFRVLTGHHVSMSDGTGIV
                     HTAPAFGEDDYRMGLKHELPMPRPVNRRGEFTDDITPWAGQFVKKADPSILDQLKESG
                     ALYTAGTFTHSYPFCWRCDTPLIYYARDSWYLKTTAYKERMLEANATIDWFPKEVGEK
                     RFGEWLENNIDWAISRDRYWGTPLPIWRCEGCGKTECMEGREALRARGADVPADLDLH
                     RPCIDRITFACASCGRTMRRVPEVVDVWFDSGAMPFAQWHYPFENRERFEVMFPADFI
                     SEGIDQCRGWFYSLLAISVAVTGKSPYRQCVVNGHVLDVAGQKMSKHKGNTVDPWELL
                     NAEGADAVRWYLMANSPPWLPTRFDKAGVTEVARKFFGTLRNVAGFFGMYANIDDWQS
                     DAAAASDTPWPFVPAAQRPVIDRWILSRLDALTADVTRHLDEHDVTRGARALQSFVLD
                     DLSNWYVRRNRRRFWKGEKGGDKSAAYATLHEVLATVSKLAAPWAPFIAEELHQKIVR
                     PVDPAAPVSVHLCDWPSAALDPTSPGGRRDVPLEEAMTAAMTIVEAARAVR"
     gene            complement(2569..3390)
                     /locus_tag="VF720_05530"
     CDS             complement(2569..3390)
                     /locus_tag="VF720_05530"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX7878848.1"
                     /translation="MTPLSRDLDEAVQAIKKKSGLAPRLGLILGTGLGDASDAIEDAV
                     SIPYHEIPHFPTSTVVSHSGQLVLGRLSGQPVVAMKGRVHFYEGYDMRQVTFPIRVMR
                     ALGAEILVASNAVGGMNPQYDPGDIVCITDHINLMGDNPLIGPNDDELGPRFPDMSEP
                     YDRKLVALAESVALEEKIKLQKGVFVAVAGPNLETRAEYRFLRAIGADVVGMSLVPET
                     IVAVHGGMRVLAFSVVTDSCLPDALEPVDIERILATAAKAEPVLKRLVASVLKKI"
     gene            complement(3387..3728)
                     /locus_tag="VF720_05535"
     CDS             complement(3387..3728)
                     /locus_tag="VF720_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX7878849.1"
                     /translation="MTATGPPIESTAAGVRFLVHVSPRASKDSVRGFHAAGRLIVRVT
                     APPVDGEANAAVVELLARQLRVSRSEVRVTRGASSRTKLIEIDGLDAATAAERLAPIP
                     ETKRTTRKDSR"
     gene            complement(3725..4309)
                     /locus_tag="VF720_05540"
     CDS             complement(3725..4309)
                     /locus_tag="VF720_05540"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX7878850.1"
                     /translation="MPKLTPLDIRKQEFPQRFRGYDPEEVDTFLDLIADDAEQITTER
                     NQLFERVGTLEAQLAEFKEIERSLRDALVMAEKMSAEVNETAQRKIESLLREAEVKSY
                     GIIGEAEAKGREIIMDAENRRRALLLELEALDSQRAYALSKFRSLLDEQRAVLEVHMM
                     SRAGTAGLPGPNGASLVPPRENANREGSPAASRA"
     gene            complement(4427..>5128)
                     /locus_tag="VF720_05545"
     CDS             complement(4427..>5128)
                     /locus_tag="VF720_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEX7878851.1"
                     /translation="VRARIQAAARASWRDPEAITLVAVSKTFPAEDVIEAVVDAIDAG
                     LAHFGKNKVQEAEAKIPIVASARTARPEWQLIGHLQSNKAGKAVELFDLIHSIDSTSL
                     AQAVARRAGMIGKRQRVLLQVNCSGEAQKSGCEPDDAADVARAIAGHTELALEGLMTI
                     GPLDDDPGSARPAFRQLRELRDRISAQLGLPLPHLSMGMTGDLEVAIAEGATIVRVGS
                     ALFGQRPAPGAAGKT"
BASE COUNT          790 a         1704 c         1733 g          901 t
ORIGIN      
        1 ctgcggacgg cccgggccgc ctccacgatc gtcatggcgg ccgtcatggc ttcttcgagg
       61 ggcacgtcgc gacgcccgcc cgggctcgtg gggtccaggg ctgcggaagg ccagtcgcac
      121 aggtgcacgc tgaccggcgc ggccggatcc accggcctca cgatcttctg gtgcaattcc
      181 tcggcgatga acggcgccca cggcgcggcc agcttcgaca ccgtggccag cacctcgtgg
      241 agcgtggcgt aggccgccga cttgtcgccc cccttctcgc ctttccagaa acggcggcga
      301 ttgcggcgca cgtaccagtt cgaaagatca tccagcacga acgactgcag cgcccgggcg
      361 ccgcgggtga cgtcgtgttc gtcgaggtgc cttgtgacat cggcagtcag tgcatccagc
      421 cgcgacagga tccagcggtc gatcacgggg cgttgcgcgg ctgggacaaa gggccagggg
      481 gtgtcggacg cggctgccgc gtccgactgc caatcgtcga tgttggcgta catgccgaag
      541 aagcccgcca cgttccggag cgtgccgaag aacttgcggg ccacctccgt gacgcccgcc
      601 ttgtcgaagc gcgtgggcaa ccagggaggg gagttggcca tgaggtacca gcgcacggcg
      661 tcggccccct cggcgttcag gagctcccat ggatcgaccg tgtttccctt gtgcttcgac
      721 attttctgcc cggccacgtc gagcacgtgc ccgttcacca cgcactggcg atacggcgac
      781 ttgccggtga cggccacgct gatggccagc aggctgtaga accagccgcg gcactggtcg
      841 atcccttccg agatgaagtc ggccgggaac atgacctcga accgctcccg gttctcgaac
      901 ggatagtgcc actgcgcgaa cggcatggcg ccggagtcga accagacatc caccacttcg
      961 ggcacgcgcc gcatcgtcct gccgcacgag gcgcacgcaa aggtgatccg gtcgatgcag
     1021 ggccggtgca ggtcgaggtc ggcgggcacg tccgcgccgc gcgcgcgcaa tgcctcgcgc
     1081 ccctccatgc attcggtctt gccgcacccc tcgcaacgcc agatcgggag cggcgttccc
     1141 cagtagcgat cgcgcgagat ggcccagtcg atgttgttct cgagccattc gccgaagcgt
     1201 ttttccccca cttccttcgg gaaccagtcg atcgtggcgt tggcctcgag catccgttcc
     1261 ttgtacgcgg tggtcttgag ataccacgag tcccgcgcgt agtagatgag cggcgtgtcg
     1321 cagcgccagc agaagggata gctgtgcgtg aaggtgccgg cggtgtagag cgcgcccgac
     1381 tccttcagct ggtcgaggat ggagggatcg gccttcttca cgaactggcc cgcccacggc
     1441 gtgatgtcgt cggtgaactc gccgcggcga ttgaccggcc gcggcatcgg cagctcgtgc
     1501 ttgagcccca tgcgatagtc gtcttcgccg aaggccggcg cggtgtgcac gatgccggtg
     1561 ccgtcggaca tggagacgtg atgtccggtg aggacgcgga acgccctggc ctccttcgcg
     1621 aagaacggga agaggggctc gtagcgcttc tcgtgcaggg tgctgccggg gaactcctcc
     1681 gcgatttcat acggcccgta tagcgcggag agtcgcgccc tggccaggat caactcagtg
     1741 ccggccgcat ggccggtatc caccttttcc acgccgtcct tgcccacgcg cggcttgagg
     1801 cgcacgcgca cgtacggctc gtccgggttg accgccagcg ccacgttgct gatgagcgtc
     1861 catggtgtgg tggtccaggc caggtacgac acattcggat cgtcggcgct ccggaacttc
     1921 acgtacaccg agggatcttc cgtgtcggcg tacccctggc tgacctcgtg cgaggagagg
     1981 ggcgtttcgc agcgcgggca gtacggaagg atcttgaacc cctcgtacac cagccccttt
     2041 tcccagaacc gggaaaggca ccaccagacc gactcgatgt agtcgttgtc gagcgtgacg
     2101 tacgggtggt ccatgtcgag ccagaagccg atgcgcgccg tcatccgctc ccactcgtcc
     2161 ttgtacgtga acaccgattc cttgcaaagc ttgttgaact tctcgaggcc gtacgcctcg
     2221 atctgttcct tcccctggat gccgagcttc ttttccacct cgagctcgac cggcaggccg
     2281 tgggtgtccc agccgctctt ccgttcgacc agatgcccct tcatggccat gtaacggcac
     2341 acgaggtcct tgatcgtccg cccgagcacg tggtggattc cgggccgtcc gttggccgtg
     2401 ggcgggcctt cgtagaagac gaagcgcggg gcgtccctgc gcatctcgag ctggcgctcg
     2461 aagacacgct cctcctgcca gaagcggagg gtggccgctt cctcgtcggc gaggagcacg
     2521 gaacgggaga attcgggaaa ccggttcatc gggatgtggc gcgctccgtc agatcttctt
     2581 caggacggag gccacgagcc gcttcaggac cggctcggct ttggccgcgg tggccaggat
     2641 ccgctcgatg tcgaccggct cgagcgcgtc cggcaggcac gagtcggtga ccacgctgaa
     2701 cgccagcacg cgcatgccgc catgcaccgc cacgatggtt tcggggacga ggctcatgcc
     2761 gaccacgtcg gccccgatgg cgcgcaggaa gcgatactcg gcgcgcgttt cgaggttggg
     2821 gccggccacc gccacgaaga cccccttctg cagcttgatc ttctcctcga gcgccaccga
     2881 ctcggccagc gcgacgagct tgcgatcgta cggctcgctc atgtcgggga agcgcgggcc
     2941 gagctcgtcg tcgttcgggc cgatgagggg gttgtcgccc atcaggttga tgtgatcggt
     3001 gatgcagacg atgtcacccg ggtcgtactg aggattcatc ccgcccaccg cgttgctggc
     3061 caccaggatt tccgcgccca gcgcccgcat cacccggatg ggaaaggtga cctggcgcat
     3121 gtcgtacccc tcgtagaagt gcacgcgccc cttcatggcc acgaccggct gccccgacag
     3181 ccggcccagg acgagctggc cggagtgcga cacgacggtg gaggtgggaa agtgcgggat
     3241 ctcgtggtac gggatcgaca ccgcgtcctc gatggcgtcc gaggcatcgc cgaggccggt
     3301 gcccaggatg aggcccaggc gcggcgccag gccgctcttc ttcttgatgg cctggacggc
     3361 ctcgtcgaga tcgcgggaga gtggtgtcaa cgtgaatcct ttcgcgtcgt gcgcttcgtt
     3421 tcggggatgg gcgcaaggcg ctcggccgcc gtggcggcgt cgaggccgtc gatttcaatc
     3481 aacttcgtac gggaggacgc accgcgcgtc acccgcacct ctgacctgga tacccggagc
     3541 tgccgggcca ggagttcgac cacggcggca ttggcctcgc cgtccaccgg cggggccgtg
     3601 acccggacga tgagcctccc cgccgcgtgg aacccgcgca ccgagtcttt cgatgcgcgc
     3661 ggcgacacgt gaacgaggaa ccggacgcct gccgccgtcg attcgatcgg cggaccggtg
     3721 gccgtcaggc ccgggacgcg gccggcgagc cctcgcggtt cgcgttctcc ctgggcggaa
     3781 ccagcgaggc gccgttcgga cccgggaggc cggcggtgcc cgcgcgcgac atcatgtgca
     3841 cttcgagcac cgcgcgctgt tcgtcgagca ggctccggaa cttggagagc gcgtaggccc
     3901 gctggctgtc gagcgcctcg agttcgagga gcagcgcccg ccggcggttc tcggcgtcca
     3961 tgatgatctc gcgacccttg gcctcggcct cgccgatgat gccgtacgac ttgacctcgg
     4021 cctcgcgaag gagcgattcg atcttgcgct gggcggtttc gttgacctcg gccgacatct
     4081 tctcggccat gaccagggcg tcgcgcagtg atcgctcgat ttccttgaac tcggcgagct
     4141 gcgcctcgag cgtgcccaca cgctcgaaaa gctggttccg ttccgtcgtg atctgctcgg
     4201 cgtcatccgc gatcaggtcg aggaacgtgt ccacctcttc cgggtcgtag ccccggaaac
     4261 gctggggaaa ctcctgcttg cggatgtcga gcggggtcag tttgggcatc ttggtgcccc
     4321 ccttcaggtt agggccggta agccccggta tcccccccgg gctcatcttc gcggcgacaa
     4381 tatagtagtg ggcccgaaaa ggtgcaagtg atgcctgtcg gacgagttag gtttttccgg
     4441 ctgcacccgg cgctggcctt tggccgaaca gcgcgcttcc cacgcgaacg atcgtagcac
     4501 cctcggcgat ggccacttcg aggtcgcccg tcatccccat cgagagatgt ggaagcggga
     4561 ggccgagttg cgcggaaatc cggtcgcgca gttcgcgaag ctggcggaag gccggtcgcg
     4621 cggacccggg atcgtcatcg agcgggccga tcgtcatgag gccttcgagc gcgagctcgg
     4681 tgtgacccgc gatggcgcgc gcgacatcgg cggcgtcatc cggttcgcaa ccgctctttt
     4741 gcgcctcgcc ggaacagttc acctggagga gcacgcgctg gcgcttgccg atcatgccgg
     4801 cgcggcgggc caccgcctgt gccagggagg tcgaatcgat cgaatggatc aggtcgaaca
     4861 gctcgaccgc cttccccgcc ttgttcgatt ggagatgccc gatcagctgc cactccgggc
     4921 gggcggtccg cgccgaagcc acgatcggga tcttggcctc ggcttcctgc accttgttct
     4981 tgccgaaatg cgcgaggccg gcgtcgatgg cgtcgacgac ggcctcgatg acatcctcgg
     5041 cgggaaaggt cttggaaacg gccaccagcg tgatggcctc ggggtcgcgc caggacgccc
     5101 gcgcggcggc ctggatccgc gcgcggac
//
LOCUS       DASVOD010000259         8416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_538578, whole genome shotgun sequence.
ACCESSION   DASVOD010000259 DASVOD010000000
VERSION     DASVOD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538578"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="VF720_05550"
     CDS             <1..1428
                     /locus_tag="VF720_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878852.1"
                     /translation="RIRAPIVFLAAWTAIIITGPARIADSTPLYAAREGRTCDNCHLT
                     PNSWVNPPLAERKCTMSCQACHVDPAGGGVRNASGRFFGRATLPMIATSPRPTQDWDR
                     GAFGMGRHDRATTYSDSLPQGPVDHAAVGAPGPYREPDDLWAHGRPAGSPSRYSLFQG
                     RYGVVAADPIFRLGWDMRLATLISGSALVFPMQADLQGVLHPVEHVTGVAAVGARGRS
                     RGFSTTLDEPESPYLRTAYVLLAEAPYQSYVKAGRFVPAYGLRLDNHTAFIRRGLHLD
                     DAIPESRVTGVEVGASPNYPYINASWFRSKAEGVAPDAFDVFDVDDSYGYAASGGWRG
                     ESIAWGASWLARRRPADEGGNETATGGWVMVNPFRGHPSFPLTWQAELDRLAFHRAGG
                     TDASGLFFYQEFDWLLGNGVNLLATQEWEDPDRAIKDDDSIRAGIGAQVTPIPGITLD
                     GRMRGLVPAGGSAGVDFILQAHFWN"
     gene            complement(1494..2630)
                     /locus_tag="VF720_05555"
     CDS             complement(1494..2630)
                     /locus_tag="VF720_05555"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7878853.1"
                     /translation="MGQILAIVSSKGGVGKTTTAVNLAAAFASLDFPTLLIEADPQCG
                     LVANFGYDRFDIPRGLADVARGECRIQDTTFETPVPGLHVVSSNVWSQEEEALYLGAL
                     ERDPLVLRRAFAELAEGYDYLMIDAPPMLGPITLAILAAADRFLVPVQAEPQSVRALA
                     RLMETSVAVRDRFNPKLRFDGIALTMVDGRTRMSQQLEDSVRREWPDQVFRATVPRSI
                     RLAEVHPSGRPLVLAAPASRGARAYAGLAEEILMAHARERAMARAMEGVTADDESVDP
                     MDPVARVDDGASTNRMADFLAALSGNGGRPANGSSRDEAPRAAAHADSSELTAALALP
                     AEEEWDERFVDLETYLEIESGGSARTAVADDDWDEDDWQERDDA"
     gene            complement(2814..4070)
                     /locus_tag="VF720_05560"
     CDS             complement(2814..4070)
                     /locus_tag="VF720_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7878854.1"
                     /translation="MRRRPLVALVLGLLIVGCGGSKTGVQPMIDEFGGYERDILALNP
                     LPPAADALKLGQAKRDQAKPLSDKGDVKASQPIMDEALADIRVGLALARADAARNKAD
                     GCLLKVDQSRESWNEVILMLEQTERVAARSISEIPREEPEMPAPPPLPPSTIDGGTVP
                     VMTTAELRPAFAAWRDLARTSGVSAGDLESRFNDRVTLADDPKGKELEVARHRYVAAR
                     TLQEMEARVREQRAMELCSQSTELSARFADARESALRATLDLERSLKDDLKHDLDAAR
                     AEAEARQSDLYESLQKLEGRFASIKKTARGTIVSLADILFDFGKATLKRDVELNLVKI
                     ATILEQYPEMKIAVEGHTDNVGSEEYNLELSEKRAKAVADFMASQGVNAERMTVAGYG
                     FSRPVADNSTDDGRQKNRRVDLVINE"
     gene            complement(4071..4835)
                     /locus_tag="VF720_05565"
     CDS             complement(4071..4835)
                     /locus_tag="VF720_05565"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015345936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX7878855.1"
                     /translation="MVIPIFFVSHWIISVFFQSFFLHRYAAHKMYTVGPRTERVIHFL
                     TWFTQGSSYLLPRAYAILHREHHAYSDSELDPHSPHQHTNVFSMMWHTKKRYGGISRR
                     TLAVEKRFLGGYPEWPLMDRIGESYIGRTAWVVAYFLFYLAFATAWWQWLLFPLTIAI
                     GPVHGAIVNWCGHKYGYRNWNTGDDSKNTLPVDVLTMGELFQNNHHRSSRRANFATRA
                     GEVDPTYQIMRLLDKMGVITLLKPRVSQADQAAKAA"
     gene            complement(4922..5878)
                     /locus_tag="VF720_05570"
     CDS             complement(4922..5878)
                     /locus_tag="VF720_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HEX7878856.1"
                     /translation="MILRPDDAAPGMTAPPTRRLGAVRSSEAGPTLLIIAGIHGNEPA
                     GVTAVRQFLAGFKADRPGFRGDIVALCGNVAACRTGQRYLDHDLNRRWSRERLMGLAL
                     AGPSDRDREDDEQLALSKEIDLVMAAARGPVYFLDLHTTSAAGYPFSMIADQPEQRAF
                     ALHFEIPIILGLLERVDGVLLEYLRRRGCTCLGIEAGQHDLSASVDHHLAALDIGVVA
                     AGLLPAGEALEASRATLREARGELPRLMRVEERHGIRPDDQFRMEPGFSNIEPIEAGQ
                     LLARDRSGEIRARKKGILFMPLYQGLGDDGFFTGRALALDNE"
     gene            complement(5875..7788)
                     /locus_tag="VF720_05575"
     CDS             complement(5875..7788)
                     /locus_tag="VF720_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7878857.1"
                     /translation="MMQLQTDFSGVERRRFMRALLADVRALERMMAEGMIEEGVRRVG
                     AEQEVFLLNKAWHVAPAADDVLPVVDDPHFTTEFGSFNLEFNMDPLVFGGDCLSRMEG
                     QIDGLLAKLRTAAASREVEVMMVGILPTIRKSDLGEENMTPRDRYRALNRALTALRGG
                     TYEFYIKGVDELLLKHDSAMLEACNTSFQVHFQAGAREFANLYNIAQVAAAPVLAAAT
                     NSPLLFGKRLWAETRIALFQQAVDTRSSGHHIREHKPRVTFGNGWLRGTAAEIFKEDI
                     ASFRALVGLEAEEDPFDILKRGGVPELNALRLFNGTVYRWNRACYGIMNGKPHLRIEN
                     RVLPAGPTVVDEVANAAFWFGVISALSDQYDDITRVIDFESASSNFVSAARIGLSAQL
                     HWLEGKEAPAQTLILDTLLPMARTALLKRDVDEKDVDRYLGIIENRVRTGRTGSKWML
                     TSLAALKDKGTPAERLGALTAASVARQKEGLPVAEWKPAELSEAGGWQHNYAVVEQYM
                     VTEIYTVQPDEPIDLVANLMEWQRIRHVPVEDQDHHLIGIVSYRAVLRALAHGQHNPD
                     GMPVAVADVMKKNPYSVAPDTPTLEAIELMRRHQIGALPVVHNGRLIGIITEHDFLDI
                     AAELLEQKLREPA"
     gene            complement(7978..>8416)
                     /locus_tag="VF720_05580"
     CDS             complement(7978..>8416)
                     /locus_tag="VF720_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020141462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7878858.1"
                     /translation="DCKDPEMEVIVAGASIGAFNSLAILARFPDVFRAAICMSGTYDM
                     DRFLPGETSDDYYYSSPLHFLPNLGGETLDRLRQRFVLLASGEGRAENIGESWRVAQL
                     LGQKGVPNRVDSWGAEWHHDWPTWRNMMPKYLEELLPAGQPAG"
BASE COUNT         1322 a         2762 c         2899 g         1433 t
ORIGIN      
        1 cgcattcgtg cgccgatcgt cttcctggcc gcctggaccg ccatcatcat taccgggccg
       61 gcgcggatcg ccgacagcac gcccctgtat gcggcacgcg aagggcgtac ctgcgacaac
      121 tgccacctga ctccgaattc ctgggtcaat ccgccgttgg ccgagcggaa gtgcaccatg
      181 tcgtgccagg cgtgccatgt cgatccggcc ggcggtggcg ttcgaaacgc ctcggggcga
      241 ttcttcgggc gcgccacgct gccgatgatc gccacgagcc cgcgccccac gcaggactgg
      301 gatcgcgggg ctttcggaat gggacgccac gatcgcgcca ccacctactc cgacagcctc
      361 ccccagggac cggtggatca cgcggccgtc ggtgcgcccg gaccctatcg cgagcccgac
      421 gacctctggg cgcacggtcg tccggcaggc tccccctccc ggtactcgct cttccagggg
      481 cggtacgggg tcgtggcggc cgatccgatc ttccggctcg gctgggacat gcggctggcc
      541 accctcatct ccggctcggc gctggtcttc ccgatgcagg ccgatctgca gggggtgctg
      601 caccctgtcg agcatgtcac gggagtggcc gccgtcggcg cccgcgggcg gagccgcggt
      661 ttctcgacca ccctcgacga gccggaatct ccctacctcc ggacggccta cgtgctcctc
      721 gcggaagcgc cgtaccagtc gtacgtgaag gccggccgct tcgtgccggc gtacgggctg
      781 aggttggaca atcacaccgc gttcatccgg cgcgggttgc acctcgatga cgccattccc
      841 gaatcacgcg tgaccggcgt ggaggtgggt gcctcgccca actatcccta catcaacgcc
      901 agctggttcc ggagcaaggc ggaaggagtg gccccggacg cattcgatgt cttcgacgtg
      961 gacgacagct acggatacgc cgcctccggc ggttggcgcg gtgaatcaat agcgtggggc
     1021 gcgagctggt tggcgcggcg ccggccggcc gatgaagggg gaaacgagac ggccaccggc
     1081 ggctgggtca tggtcaatcc gttccgcggg catccctcgt tcccattgac ctggcaggcg
     1141 gaactggatc ggctcgcgtt tcaccgtgcc ggaggcaccg acgcatcggg ccttttcttc
     1201 taccaggagt tcgactggct ccttggcaac ggcgtgaacc tgctggccac acaggagtgg
     1261 gaggacccgg atcgcgccat caaggacgac gattcgatcc gcgcggggat cggcgcccag
     1321 gtcaccccga tacccggtat cacgctggat ggccggatgc gtgggctggt tcctgccggc
     1381 ggcagcgcgg gcgtggactt catcctgcag gcccacttct ggaactgacc cgaagccact
     1441 tctggaactg accggaggcc accgcccgga attatcggcg tgattcggcc cgctcaggcg
     1501 tcgtcgcgtt cctgccagtc gtcctcgtcc cagtcgtcgt cggccaccgc ggtgcgggcg
     1561 ctgccgcccg attctatttc caggtaggtc tcgaggtcga cgaagcgctc gtcccactct
     1621 tcctcggcgg ggagcgccag ggcggcggtg agttcgctgg aatcggcgtg cgcggcggca
     1681 cgcggcgcct catcccgcga agatccattg gccggtcggc cgccgttgcc ggagagcgcc
     1741 gccaggaaat cggccatgcg gttggtggag gcgccgtcat cgacgcgagc caccggatcc
     1801 atcggatcga cggactcgtc gtcggccgtc accccctcca tggcccgggc catggcccgc
     1861 tccctggcgt gcgccatgag gatctcctcg gccagcccgg cataggcgcg ggcgccgcgc
     1921 gaggccggcg ccgcgagaac cagcgggcgg ccggaggggt gcacctcggc caggcggatg
     1981 ctgcgcggca ccgtggcccg gaagacctgg tccggccact cgcgccgcac ggagtcttcg
     2041 agttgctggc tcatgcgcgt gcggccgtcg accatcgtga gcgcgatccc gtcgaagcga
     2101 agcttcgggt tgaagcggtc gcgcaccgcc accgaggttt ccatcaggcg ggccagcgcg
     2161 cgcaccgatt gcggttcggc ctgcaccggc acgaggaatc ggtccgccgc cgccaggatg
     2221 gccagcgtga tggggccgag catcggcggg gcatcgatca tgaggtagtc gtacccctcg
     2281 gcgagttcgg cgaaggcgcg ccggaggacg agcggatcgc gttcgagggc gcccaggtag
     2341 agcgcctctt cctcctggct ccagacgttg ctcgatacca cgtgcaggcc cgggaccggc
     2401 gtctcgaagg tggtgtcctg gatccggcac tcaccgcgcg ccacgtcggc aaggccgcgt
     2461 ggaatgtcga aacggtcgta gccgaaattg gccaccaggc cgcactgcgg atccgcttcg
     2521 atgaggagcg tggggaaatc gagcgaggcg aacgccgcgg ccaggtttac cgccgtggtc
     2581 gtctttccca cgcctccctt ggacgacacg atcgccagaa tctgtcccat gactctcccg
     2641 gatgcggtcc cacggcgttg gccgggggcc atttcaaaac gttcggggcg ggtccggcgt
     2701 aatccggacg cgccccgaac gcggtgtgtg gaaagaactt cgggattcaa ccataccgtc
     2761 gcgcacttgt gaagtgaaat tcgaacggac gcgacgggat ttgaatcgga tggttactcg
     2821 ttgatgacga gatcgacccg acggttcttc tgacgcccgt cgtcggtgga gttgtcggcc
     2881 accggccgcg aaaacccgta gcccgccacc gtcatccgct cggcattcac cccctgcgag
     2941 gccatgaaat cggccaccgc cttcgcccgc ttctcggata gctcgaggtt gtattcctcg
     3001 gatccgacgt tgtcggtgtg accttcgacg gcgatcttca tttcggggta ctgctcgagg
     3061 atcgtggcga tcttcacgag gttgagctcg acatcccgct tgagcgtggc cttgccgaag
     3121 tcgaacagga tgtccgccag gctcacgatc gtgccgcggg ccgtcttctt gatgctggcg
     3181 aagcgccctt ccagcttctg gagcgattcg tacaggtcgg actgacgcgc ctcggcctcg
     3241 gcccgggcgg catccaggtc atgcttcaaa tcgtccttga ggctccgttc cagatcgagc
     3301 gtggcccgga gcgccgattc cctcgcgtcc gcgaagcggg ccgagagctc ggtcgactgg
     3361 gagcagagct ccatggcgcg ctgctcgcgc acccgggcct ccatctcctg aagggtccgg
     3421 gcggccacgt aacggtgccg ggccacctcc agctccttcc ccttggggtc gtcggccagg
     3481 gtcacccggt cgttgaaacg gctctcgagg tctccggcgg atacgccgga ggtgcgggcc
     3541 aggtcgcgcc aggcggcaaa tgccgggcgc aactcggcgg tggtcatgac cggcacggtt
     3601 ccgccgtcga tggtggacgg ggggagcggg ggcggggccg gcatctccgg ttcctccctc
     3661 gggatctccg aaatgctccg ggcggccacg cgttcggtct gctcgagcat caggatgact
     3721 tcgttccagc tctcgcggga ctggtcgacc ttgagcaggc agccgtcggc tttgttccgg
     3781 gccgcgtcgg cgcgcgccag ggccaggccg acgcggatgt cggccagtgc ctcgtccatg
     3841 atcggctgcg aggccttgac gtcccccttg tcggacagag gcttggcctg gtcacgcttt
     3901 gcctggccga gcttgagcgc gtcggccgcc gggggaaggg gattgagcgc caggatgtcg
     3961 cgttcgtagc cgccgaactc gtcgatcatc ggctgaacgc ccgtcttgga gcctccgcag
     4021 ccgacgatga gaagtccgag gaccagggcg acgagagggc gtcgccgcac ttatgccgcc
     4081 ttcgccgcct ggtcggcctg gctgacccgc ggcttcagga gcgtgatgac gcccattttg
     4141 tcgaggagcc gcatgatctg gtaggtcgga tcgacctctc ccgcccgcgt ggcgaagttg
     4201 gcccgccggc tggaacgatg gtggttgttc tggaagagct cacccatggt caacacgtcg
     4261 accgggagcg tgttcttgct gtcgtcgccc gtgttccagt tccggtagcc gtatttgtgg
     4321 ccgcaccagt tgacgatggc gccgtgaacc ggaccgatgg cgatggtgag cgggaagagg
     4381 agccactgcc accaggccgt ggcgaatgcc aggtagaaaa ggaaataggc caccacccac
     4441 gccgtccggc cgatgtacga ctcgccgatc cggtccatga ggggccactc cgggtaaccg
     4501 cccaggaagc gcttctcgac ggccagcgtc cggcgggaga tgccgccgta gcgcttcttg
     4561 gtgtgccaca tcatcgagaa gacgttggtg tgctggtgcg gcgagtgggg atcgagctcg
     4621 ctgtcggagt aggcgtggtg ctcccggtgc aggatggcgt aggcgcgcgg gaggaggtac
     4681 gaggaaccct gggtgaacca ggtcaggaaa tgaatgaccc gctcggtgcg tggccccacc
     4741 gtgtacatct tgtgcgcggc gtaccgatgc aggaagaaac tctggaagaa aaccgagatg
     4801 atccaatgac tcacgaagaa aattggaatg accaaaatcg tgtcctttcc ggctcgccgt
     4861 gctgcttcgg gcggcccgtc aactgcgatg ccggaaccac ccggcatcgc ccttcggtat
     4921 cttactcgtt atcgagggcc agggcgcgac cggtaaaaaa tccatcgtcg cccagcccct
     4981 ggtaaagcgg catgaacagg atgcccttct tccgggcacg aatctctccc gaccggtcgc
     5041 gggccaggag ctgccccgcc tcgatcggct cgatgttgga aaaaccgggc tccatccgga
     5101 actggtcgtc cgggcggatg ccgtgacgct cttctacgcg catgaggcgg gggagttcgc
     5161 cgcgggcctc gcggagcgtg gcgcggctgg cctcgagcgc ctcgccggcg ggcaaaagcc
     5221 cggcggccac caccccgatg tcgagcgccg caaggtggtg atccaccgag gccgacaggt
     5281 cgtgctgccc cgcctcgatg ccgaggcagg tgcacccccg ccggcggagg tattcgagga
     5341 gcacgccgtc cacccgttcg aggagtccca ggatgatcgg gatctcgaaa tggagggcaa
     5401 aggcccgctg ctcgggctgg tcggcgatca tgctgaacgg gtagccggcc gccgacgtgg
     5461 tgtgcaggtc gaggaagtac accggtcccc tggcggcggc catcaccagg tcgatctcct
     5521 tcgacagggc cagctgctcg tcgtcctcgc gatcccggtc cgaggggccg gccagggcaa
     5581 gacccatcag gcgctcgcgg ctccagcggc gattcaggtc gtggtcgagg tagcgctggc
     5641 cggtccggca ggcggccacg ttcccgcaga gcgccacgat gtccccccgg aagccggggc
     5701 gatcggcctt gaagccggcc aggaactgtc ggacggccgt gactccggcc ggctcgttgc
     5761 cgtggatccc cgcgatgatg aggagcgtgg ggccggcctc gctcgagcgc accgcgccca
     5821 ggcgccgcgt cggcggtgcc gtcattccgg gagccgcgtc gtcgggtcgc aagatcacgc
     5881 cggctcccgc agcttctgtt ccaggagctc ggccgcgatg tcgaggaagt cgtgctcggt
     5941 gatgatgccg atcaggcgac cgttgtgcac gacggggagg gcgccgatct ggtggcgccg
     6001 catcagttcg atggcctcga gcgtgggcgt gtccggcgcc accgagtacg gattcttctt
     6061 catgacgtcg gccacggcca cgggcatgcc atccgggttg tgctgaccgt gtgccagggc
     6121 ccgcagcacc gcccggtacg acacgatgcc gatcaggtga tggtcctggt cttccaccgg
     6181 cacatggcga atgcgctgcc actccatgag gttggccacg aggtcgatcg gctcgtcggg
     6241 ctgcacggtg tagatctcgg tgaccatgta ctgctcgacc accgcgtagt tgtgctgcca
     6301 accccccgcc tcgctcagct cggccggctt ccactcggcc accgggagcc cttccttctg
     6361 gcgggccacc gatgcggcag taagggcgcc cagccgctcg gcgggcgttc ccttgtcttt
     6421 cagcgccgcc agcgaggtga gcatccactt gctcccggtc cgaccggtgc gcacccggtt
     6481 ctcgatgatg ccgaggtagc ggtcgacgtc cttctcgtcc acgtcgcgct tgagaagggc
     6541 cgtgcgggcc atcggcagga gcgtatcgag gatcagggtc tgggcgggcg cttccttccc
     6601 ctcgagccag tggagctggg ccgagaggcc gatgcgggcg gccgacacga agttcgacga
     6661 ggccgactcg aagtcgatca cccgggtgat gtcgtcgtac tggtcggaca gggcgctgat
     6721 gacaccgaac cagaaggccg cgttggccac ttcgtccacc accgtcgggc cggccggcag
     6781 cacccggttc tcgatacgga ggtgcggctt gccgttcatg atgccgtagc aggcccggtt
     6841 ccaccggtag accgtgccgt tgaagaggcg aagggcgttc aactcgggca cgcccccccg
     6901 cttcaggatg tcgaacgggt cttcctccgc ctcgaggccg accagggccc ggaaactggc
     6961 gatgtcttcc ttgaagatct cggcggcggt gccgcgcagc cagccgttgc cgaacgtgac
     7021 ccggggcttg tgctcgcgga tatggtggcc gctggaacgg gtgtccaccg cctgctggaa
     7081 gagggcaatg cgcgtctcgg cccagaggcg cttcccgaag aggagcgggc tgttggttgc
     7141 agcggccagg acgggcgccg cagccacctg cgcgatgttg taaaggttgg cgaactccct
     7201 ggcgccggcc tggaagtgca cctggaagct ggtattgcag gcttcgagca tggccgaatc
     7261 gtgcttcagg aggagctcgt caaccccctt gatgtagaac tcgtaggtgc cgccgcgcag
     7321 ggcggtgagg gcccggttca gcgcccggta gcggtcccgg ggggtcatgt tctcttcgcc
     7381 caggtccgat ttccggatgg tgggaaggat gcccaccatc atgacttcca cctcgcggga
     7441 ggcggccgcc gtccggagtt tggccaggag gccgtcgatc tgcccctcca tccgggagag
     7501 gcagtccccg ccgaagacga gcgggtccat gttgaactcg aggttgaagc tcccgaattc
     7561 ggtggtgaag tgggggtcgt cgacgaccgg caggacgtcg tcggccgcgg gggccacatg
     7621 ccaggccttg ttcaggagga agacctcctg ctcggcgcct acccgccgga ccccttcctc
     7681 gatcataccc tcggccatca ttcgctccag ggcccggaca tcggcaagga gcgcccgcat
     7741 gaaacggcgg cgttcgactc ccgaaaagtc ggtctggagt tgcatcatgc ccaagcgaga
     7801 ttccccctaa aattttgcga ggctgtacct tagccgaaac gacagccaga agcaacgaag
     7861 cggggaggag cgggccggga gagggggcag gataggaatg aattcatgct gcagttgaat
     7921 tcagtcggcg gacccgtgta aaaaggtcgg tccgtccacg tggaggaagt ccgtcgatca
     7981 gccggccggc tgccccgccg gaagcagctc ctccaggtac ttgggcatca tgttccgcca
     8041 ggtgggccag tcatgatgcc actccgcgcc ccacgaatcg acccgattgg gcaccccctt
     8101 ctgcccgagt agctgggcca cgcgccacga ctcgccgatg ttctcggcgc gcccctcgcc
     8161 cgaggcgagg aggacgaagc gctgacgcag gcggtcaagc gtttccccgc caaggttcgg
     8221 caggaaatgg aggggcgagg aatagtaata gtcgtcactc gtttcgccgg ggaggaagcg
     8281 atccatgtcg tacgtgccgc tcatgcagat ggcggcccgg aagacatcgg gaaagcgggc
     8341 caggatggcc aggctgttga acgcgccgat cgacgcgccg gccacgatca cttccatctc
     8401 gggatccttg cagtcc
//
LOCUS       DASVOD010000260         4555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_279094, whole genome shotgun sequence.
ACCESSION   DASVOD010000260 DASVOD010000000
VERSION     DASVOD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279094"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1039)
                     /locus_tag="VF720_05585"
     CDS             complement(44..1039)
                     /locus_tag="VF720_05585"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX7878859.1"
                     /translation="MTDYFNDLFAQYSETIFRWVAHSGLRVLLIVVGAFLVVQIAFRL
                     IKRIETLADDGDPNFQSLQEKRAATLSSLLRQVTGVIIAIVSGMMILREFRVDIAPII
                     AGAGIVGLAVGFGAQSLVKDIISGFFILLENQFDVGDVISGAGVSGGVERIGLRFTQL
                     RDLEGRVHFVPNGEFRVVSNLTRGWSRAVLDVAIGYREDIDRVQGVLREVSEELRHDP
                     EFDHLILEPMEVLGVDSFGESAVTIRLLFKTLPGKQWPVAREFRKRIKMKFDALGIEF
                     PFPQRVVHTVALPSGGTLVLDEGAAPPRGDRGADPARAEAGASQSTVARLPDTND"
     gene            complement(1056..2051)
                     /locus_tag="VF720_05590"
     CDS             complement(1056..2051)
                     /locus_tag="VF720_05590"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7878860.1"
                     /translation="MRIAVLMGGTSSERQVSLASGRGVVAGLKGAGHEVTAIDAADGH
                     VFSDAELAALKISVEPPRPQKGSRRALATCAPLLNAELAFIIVHGGEGEDGTLQAFLE
                     MMDIPYTGSGMRACALTWDKEIARRMMAADSIPVAAGFLAPLGMTPAEVRARIDAEVG
                     WPAIVKPNAQGSTIGVTRLDTADGLAAALEKAAVNGGDALIERFIPGHELTVTVFDGE
                     VFPVTEIVADTGFYDYERKYQKGHTRYVTPAEISAERAAEAQRLTGRIFQLFGCRGVA
                     RVDFRMTPEGDLYCLEINTVPGMTEMSLVPMGARALGIDYPELVDRMARAALAAR"
     gene            complement(2053..2541)
                     /gene="ispF"
                     /locus_tag="VF720_05595"
     CDS             complement(2053..2541)
                     /gene="ispF"
                     /locus_tag="VF720_05595"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX7878861.1"
                     /translation="MTHRIGFGYDAHRFKAGRPLMLGGVAIPFEMGLEGHSDADVLTH
                     AIADAILGALGDGDIGQHFPPGDPNWKDAASLKLLAHVMTWARSRKARVVNVDATLVL
                     EAPKIAPHAAAMRKAIGEVLGIPAERVSIKATTNEGMGAVGRGEGAVAHAVVLLELET
                     EE"
     gene            complement(2538..3284)
                     /gene="ispD"
                     /locus_tag="VF720_05600"
     CDS             complement(2538..3284)
                     /gene="ispD"
                     /locus_tag="VF720_05600"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX7878862.1"
                     /translation="MTDHDDSLTMQVAVILVAAGRGERSGRRDKVLQRLGDRTVLAQA
                     IAPFLEEPRVTTVVLVVPPGREAEFEEAAFPDGPPAGRIVRVVPGGERRQDSVANGLA
                     ALGPEIELVAVHDAARPLHRHDMLVRLIETAATVGAAVPAVPPRDTIAQVDEGSGLLA
                     AAGLERGRLRAIQTPQIFRRDWLMAAHATAAADRLEATDDAGLVRRLGKPVAFVPGEA
                     DNIKLTLDSDFELAAALLKARGNTAKDDHP"
     gene            complement(3281..>4555)
                     /gene="radA"
                     /locus_tag="VF720_05605"
     CDS             complement(3281..>4555)
                     /gene="radA"
                     /locus_tag="VF720_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEX7878863.1"
                     /translation="GGAAPEPTDLSRLHATPLERIETGIGEFDRVLGGGFVPGEAILL
                     GGDPGIGKSTLLLQVATELVRNGIRVLYVSGEESAGQIHLRARRLGAIPDGLFVVAET
                     DLGAVLDLLHERRPTVAIIDSIQTMRHAELGSIPGSVAQVRECALSLIGQAKSGGAAV
                     ILVGHVTKDGLVAGPKTLEHMVDAVVHLEGDRFHHYRILRAAKNRFGATHEVGVFEMK
                     DDGLAEVPNPSEALLGERGEPASGVAVVASLEGTRPLLVEVQALVAPKAFSYPQRVTT
                     GFEARRLALLLAVLGQRCGVDLTERDVFVSVAGGFTLDDPAVDLGMAVAVAGAHRGLP
                     LDHRTVYLGEIGLGGEIRRVKRADLRLREAQRLGFACGVLPAANEAECRMAKDAGLDL
                     IQVKTLGQALDAVFGPATGGKRGGRTTVRSEA"
BASE COUNT          689 a         1616 c         1576 g          674 t
ORIGIN      
        1 ggtcagcgtg tcgtagatgc ggatggccaa ggagttctcc tggtcagtcg ttcgtgtcgg
       61 gaaggcgggc caccgtcgac tgactggccc cggcctcggc acgggccgga tcggcccccc
      121 gatcgccccg cgggggcgcc gcgccctcgt ccaggaccag ggtgccgccc gacggaagcg
      181 cgacagtatg caccacgcgc tgcgggaacg gaaactcgat cccgagggca tcgaacttca
      241 tcttgatgcg cttccggaac tcccgggcca ccggccactg cttcccggga agcgtcttga
      301 acaggaggcg gatcgtgacc gccgattcac cgaacgagtc gacgccaagg acctccatgg
      361 gctcgaggat caggtggtcg aactccgggt cgtgccgaag ctcctcggac acctcgcgca
      421 gcacgccctg cacgcggtcg atgtcctcgc ggtagccgat ggccacgtcg agcaccgcgc
      481 gcgaccagcc gcgcgtgagg ttggagacga cgcggaactc gccgttcggg acgaagtgga
      541 cgcgcccttc caggtcgcga agctgggtga atcggaggcc gatccgctcg acgccccccg
      601 agacgccggc gcccgagatc acgtccccca cgtcgaactg gttctcgagg aggatgaaga
      661 agccggagat gatgtccttg accaggctct gcgcgccgaa gcccaccgcc aggcccacga
      721 tgccggcgcc ggcgatgatg ggggcgatgt ccacgcggaa ctcgcgcagg atcatcatcc
      781 ccgacacgat ggcgatgatc accccggtca cctggcgcag caggctcgac agcgtggcgg
      841 cccgcttctc ctgcaagctc tggaagttgg gatcgccgtc gtcggccagc gtttcgatac
      901 gcttgatcag ccggaaagcg atctgaacga cgaggaacgc gccgacgacg atgagcagga
      961 cgcgcaggcc cgagtgggcc acccagcgga agatggtttc cgagtactgg gcgaagagat
     1021 cgttgaagta gtcggtcatg tcgcgaggcc gccgctcatc gcgcggccag ggccgcccgg
     1081 gccatccggt ccaccagctc cgggtagtcg atgccgagcg cccgggcgcc catcggcacc
     1141 agcgacatct cggtcatccc cggcacggta ttgatctcga ggcagtagag atctccctcg
     1201 ggcgtcatgc ggaagtcgac gcgcgccacg ccgcgacaac cgaagagctg gaagatgcgc
     1261 ccggtgaggc gctgcgcctc cgcggcccgc tcggccgaga tctcggcggg cgtgacatag
     1321 cgcgtgtgcc ccttctggta cttccgctcg tagtcgtaga acccggtgtc ggccacgatc
     1381 tcggtgaccg ggaagacctc gccatcgaac acggtgacgg tgagctcgtg ccccgggatg
     1441 aagcgctcga tcagcgcgtc gccaccgttg acggcggcct tctccagcgc ggcggccagg
     1501 ccgtcggcgg tatcgagcct ggtgacgccg atggtggagc cctgggcgtt cggcttcacg
     1561 atggccggcc agccgacctc ggcgtcgatc cgggcgcgga cttcggccgg cgtcatgccg
     1621 agcggcgcca ggaagcccgc cgccaccggg atgctgtccg cggccatcat ccgccgcgcg
     1681 atctccttgt cccaggtcag cgcgcaggcg cgcatgccgg agccggtgta cgggatatcc
     1741 atcatctcga ggaacgcctg cagcgtgccg tcctcccctt cgccgccgtg cacgatgatg
     1801 aaggcgagtt cggcattgag aagaggcgcg caggtggcca gcgcgcggcg cgatcccttc
     1861 tgcgggcgcg gcggttccac gctgatcttg agcgcggcca gctcggcatc cgaaaagacg
     1921 tgcccgtcgg ccgcgtcgat ggccgtcacc tcgtgccccg cgcccttgag ccccgccacc
     1981 accccgcgcc ccgaggccag gctcacctgc cgctcggaac tcgtcccccc catcagtacc
     2041 gcgatgcgca ccctactcct ccgtttccaa ttccaggagg acgaccgcgt gagccacggc
     2101 gccctcgccg cgtcccaccg cccccatccc ctcgttcgtc gtggccttga tcgacacccg
     2161 ctccgccgga atccccagca cctcgccgat ggccttccgc atggccgccg cgtgcggcgc
     2221 gatcttcggc gcctcgagca ccagcgtggc atcgacgttc acgaccctgg ccttgcgact
     2281 cctggcccag gtcatcacgt gggccaggag cttcaggctg gccgcgtcct tccagttcgg
     2341 atcgcccggc gggaagtgct ggccgatgtc gccgtcaccg agcgcgccga gaatggcatc
     2401 ggcgatggcg tgcgtgagca cgtcggcgtc cgagtgtcct tccaggccca tctcgaacgg
     2461 aatggccacg ccccccagca tgagggggcg ccccgccttg aaccggtggg catcgtatcc
     2521 gaagccgatg cggtgggtca tgggtggtcg tcctttgccg tattgccccg cgccttgagc
     2581 agcgccgccg ccaattcgaa gtccgaatcg agcgtgagct tgatgttatc cgcctcgccg
     2641 ggcacgaacg caaccggctt tccgaggcgt cggacgaggc cggcgtcgtc ggtggcctcc
     2701 aaccggtccg cggcggcggt ggcatgggcc gccatcaacc agtcgcgccg gaagatctgg
     2761 ggggtctgga tggcgcgcag gcgcccgcgc tcgagcccgg ccgcggccag gagcccgctc
     2821 ccctcgtcga cctgggcgat cgtgtcccgc gggggaacgg ccggcacggc cgcgccgacc
     2881 gtggcggcgg tctcgatcaa ccggaccagc atgtcgtggc gatggagggg acgtgcggcg
     2941 tcatgcacgg ccaccagctc gatttccggc ccgagggctg cgagcccgtt ggccaccgaa
     3001 tcctgccgcc gctcgccgcc gggcaccacg cggacgatgc gcccggccgg gggtccgtca
     3061 ggaaaggcgg cttcctcgaa ctcggcttcg cgcccgggcg gtaccacgag aactaccgtc
     3121 gtgacccgcg gctcctcgag gaaaggcgcg atcgcctgcg ccaggacggt gcgatcaccc
     3181 agccgctgca gcaccttgtc ccggcggccc gaacgttcgc cccgaccggc ggccaccagg
     3241 atgacggcca cctgcatcgt gagcgaatcg tcgtggtcgg tcatgcctcg ctccgtacgg
     3301 tagtgcggcc gccgcgcttg ccgccggttg cgggtccgaa gacggcatcg agcgcctgtc
     3361 ccagcgtctt cacctggatg aggtcaaggc ccgcgtcctt cgccatccgg cactcggcct
     3421 cgttggccgc gggcaacacg ccgcacgcga agccgagacg ctgcgcttcg cgcagtcgga
     3481 ggtcggcgcg tttcacgcgc cggatttcac cacccaggcc gatctccccc aggtacaccg
     3541 tgcgatggtc gagcggcagg ccccggtgag cgccagccac ggccacggcc atgccgagat
     3601 cgaccgcggg gtcgtcgagc gtgaagccgc cggccacgct gacgaagaca tcgcgctcgg
     3661 tgagatccac gccgcagcgc tggccgagga cggcaaggag cagcgccagt cgccgcgctt
     3721 cgaagccggt ggtgacccgc tgcgggtagc tgaaggcctt gggtgcgacc agcgcctgca
     3781 cttccacgag gagcggccgc gttccctcga gactggccac gacggccacg ccgctggcgg
     3841 gctcgccccg ctcgccgagc agcgcctcgg acgggttcgg cacctcggcc aggccgtcgt
     3901 ccttcatctc gaagacgccc acttcgtgcg tggcgccgaa gcggttcttg gccgcgcgca
     3961 ggatgcggta gtggtggaag cgatcccctt cgaggtgcac gaccgcatcg accatgtgtt
     4021 caagcgtctt cggcccggcc acgaggccgt ccttggtgac gtggcccacc aggatgaccg
     4081 ccgcgccgcc cgacttggcc tggccgatga gcgagagtgc gcactcgcgc acctgggcca
     4141 cgctgccggg gatggagccg agttccgcat ggcgcatcgt ctggatggag tcgatgatgg
     4201 ccacggtggg gcggcgctcg tggaggaggt cgagcacggc accgaggtcg gtttcggcca
     4261 cgacgaagag gccatcggga atggcgccga gccgccgggc gcgcaggtgg atctggccgg
     4321 ccgattcctc gccgctcacg tacaggacgc ggatgccatt gcgcacgagc tcggtggcca
     4381 cttgcagtag gagcgtggac ttgccgatgc ccggatcgcc gccgagaagg atggcctcgc
     4441 ccggaacgaa gccgccgccg aggacgcggt cgaactcgcc gatgccggtc tcgatccgtt
     4501 cgagcggcgt ggcgtggagg cgcgaaaggt cggtgggttc aggtgcggcg ccgcc
//
LOCUS       DASVOD010000261        14864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_747731, whole genome shotgun sequence.
ACCESSION   DASVOD010000261 DASVOD010000000
VERSION     DASVOD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14864
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747731"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF720_05610"
     CDS             <1..184
                     /locus_tag="VF720_05610"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878864.1"
                     /translation="VLDVRGRAVRTLHDGALEGGEFTWPWSGEDDDGRDVPNGVYLVI
                     VDAGSDRSARKIVLAR"
     gene            421..1617
                     /locus_tag="VF720_05615"
     CDS             421..1617
                     /locus_tag="VF720_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEX7878865.1"
                     /translation="MTTPYADIFRRAHTFTKAQEARASGFYPYFIPISGSDGTEVNIG
                     GQNRIMIGSNNYLGLTHDPRVLAAAEKAAREYGSGCTGSRFLNGTLDLHLKLEHDLAE
                     FMGYEAALVFSTGFQTNLGTIATLVTKDDAVIIDKLDHASIVDGCFMSPGKVFRYRHN
                     DMEDLERQLNRIEPERGRLIAVDGVFSMEGDIAPLPEIVRIARKYGARILCDDAHSLG
                     VLGSRGDGTPAHFGLTKEIDLVMGTFSKSFASIGGVIAGDAMVIDYIKHFARPMIFSA
                     ALPPYAVATVHECLNIMKAEPERRERLWQITRKMLDGFKSLGFDTCTSETPIIPIMTG
                     DKEKTFYFWRSLFEAGIFANAVIPPAVPENACRIRTSYMATHTDEQLDIVLETFGKIG
                     RQVGII"
     gene            1627..2754
                     /locus_tag="VF720_05620"
     CDS             1627..2754
                     /locus_tag="VF720_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7878866.1"
                     /translation="MPPPELTVHPVAPGRDFRRFALLPWDIYAGDPYWVPPLLIDFNK
                     LFDTAKHPFHRHSEAQPFLAVRGGKPVGRITAIWNRNHQKFHGEDAGFFGFFECVDDA
                     DVAAALLEVAAAWLKERGLTVMRGPANFSSNEEWGLLVDGWNGTPKVMMPYNPRWYPA
                     LFDKAGLRTAKDLVAYFLPHNQIPERVARGTALVRRRSPDVTIRSINMKKFEAEVGII
                     RDIYNASWEKNWGFVPMTDPEIEHMARELKPVVDPDLVFFAEKAGEVIGFSLSLPDVY
                     PALQKANGRLLPFGIVKLLWYARQAEWVRVLTLGLLPEHRKSGVDVMMYDHLFKVCQR
                     KGYRGGEFSWLLEDNLAIRTAMDNLGGYVYRTYRLYEKEIA"
     gene            2751..3731
                     /locus_tag="VF720_05625"
     CDS             2751..3731
                     /locus_tag="VF720_05625"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7878867.1"
                     /translation="MKALVTGATGFIGGHLVEALLDRGHEVVALVRDPARLGPLRDHP
                     RVTSTIGDITKPETINAASFDVDVVYHLAAALRARNDADYAAINTRGTEAIARAVAAA
                     PSRPRLVYVSSLAAGGPSPGDSVLVGTETPHPVSSYGRTKLAGEAVIRKVLGKEHPWL
                     MFRPPPVYGPRDKDVFLLFKTVNGGVLPIPGDGGQVLPMLHVADLAEALVLAGTSESA
                     GHTWYITDGGLYPFRDVLSALARALNRKPLRLNVPMWILRIGGSIGQWLYDTTGRPMA
                     LNNDKVIEIEAPAWRCDPTPAFRALNFTPRWSLDEGLAQTARWYRENGWL"
     gene            3728..4612
                     /locus_tag="VF720_05630"
     CDS             3728..4612
                     /locus_tag="VF720_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7878868.1"
                     /translation="MTPSSTMRDPAPGRYRLIDKVALGYFAIGTLLLAHPNRPDNWLT
                     LLLFHAIYLVGIPAAVRWSNRSLLLSLFRDWYPVVGIMAMYGELQFLNRVLTDRYFDP
                     VVMEWEEAIFGRQMAVHLREMIPSKLFGEAVHFGYFCYYLTIPSLLIPLWLLRKYREF
                     RISVSVIGAVYIACYLWYLFWPVTGPYWQLPKPDPSTEGWLFPQITNFFVANGSSRGS
                     AFPSSHVAASITVLGMARLYLKPVYRILFLPVMLLITGTVYGGFHYAIDAIAGLALGL
                     TFDIWGPKVVTKVDGKAI"
     gene            complement(4619..7294)
                     /locus_tag="VF720_05635"
     CDS             complement(4619..7294)
                     /locus_tag="VF720_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7878869.1"
                     /translation="MSLAAGTRLGPYEITSKLGEGGMGEVYLARDTRLERDVAIKVMP
                     HHLKESAEARARFEREARAVSSLNHPNICTLHDVGRENGIDFLVMERVDGETLADRLA
                     RGPLPIPQLLRIGIEIADALDRAHRSGLVHRDLKPGNVMLARSGAKLMDFGLARARAS
                     LDSSPGMSAAATQSPTMAQPVTEKGMIVGTFQYMAPEQFEGKEADARSDLWALGCVLY
                     EMATGQRAFTGQSRAGLISAIMAGEPAPMSRMAPLTPPAFDRLVRACLEKDRDERIQS
                     AHDVKLQLRWIVEGEASGVSAVSAGVVSRRRGLNPLWAAGAALALVGLTIAGTLALHK
                     PRVEREAVHFSVVRPPGLTAFGGPAVSHDGRQLAFVGWDSSDVQRLWLRRLDSIEAHQ
                     VSSAPAGAPIWSPDDASLAFVADDKLVRIGVGGGAVVPIADVHGRLPCSWSPAGTILL
                     EPTGRDSLVAVSVADGTMTPASRLDRARGDLYHFSARFLPDGRHFLFVAARSKEGGTV
                     TASIMLGRLGSLEAEELGPCDGYVAFDPPNHVIFLRGTTLVARTLDLGRKALTGEFMT
                     LAEDVPPFTNGLIYSAGGGVVVLVNVTGLISELVWTDRTGRRLGKLGEPDRYRELDLS
                     PDGKRLAVSIEDPASGNDDIWVRDIDRGISTRFTFSPAQETSPIWSADGTRIFYSTDR
                     QGGNYLTYSFLVNSPGQEDTLRVGNYGHEGPLSVSPDGRWLAVLMSVGTTAMDDWNIV
                     IRDVQGRETPRPFCVTPAIEASAAFSPDGRWLAYGSEETGRGELYVRSFPDGARKWRV
                     SNAGGSTPHWARHGTELLYQNANSDIVSVPVTPGSEFQPGTPEVLFHADLNARGWVVN
                     RWAVTSDGERFLLNQAIKNTNPGFTVMKNWDAGLR"
     gene            complement(7344..8522)
                     /locus_tag="VF720_05640"
     CDS             complement(7344..8522)
                     /locus_tag="VF720_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7878870.1"
                     /translation="MHWGLLSLAFAYGLRMLGLYLVLPILSPYAASLPGGATFWVGMA
                     VGSYGFAQTAFQVPFGAFSDHYGRRRTIVGGLLLFVVGSLVAAMAKTAPLLVLGRTLQ
                     GAGAISSAVMALVGDLARPEYRQRAMSLLGIVIGVTFGLGLTLGPILAVKLSVPGLFY
                     ICAVMGALSAVAILVFVPSGRDAPHPPRPTFRQTIGVLHDLTLARVNLAVFALHLGLT
                     ALFVVLPIQIETLIPREQMWRVLAPAIVVGVAALAATAFLVDRFRAERLFLVIGALLL
                     VVGSLILRGGYERLGGITFGLIVVVTGIAVLEPILPSLVTLYAGREVRGLAAGTYSMC
                     QFFGAGLGGLTGAYFLARIPADIGWLVAAAGVVIALASIGLPPPHPGEHAGHRGERTG
                     "
     gene            8693..10849
                     /locus_tag="VF720_05645"
     CDS             8693..10849
                     /locus_tag="VF720_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7878871.1"
                     /translation="MSPRPAVAFAIALTLSSLPLSGAFAEDSDSNRMILMDDKNPPVA
                     KVEPHPVTLHGDTRVDNYFWLRDDKRQDPQVISYLEAENNYTARVMKPTEALQQRIFE
                     EIKSRVKETDLTVPYREKGYFYYSRTEEGKQYPIFCRRAGSIDGPEEKYCDENEMAKG
                     HDYFSLGVLDVSPDGKLMAYSTDTDGSESYVLRFRNLASGQEYPESVKDVDGSVWAND
                     NRHVFYTTRDSAKRSYRLWRHELGTSPEKDTMLYQEDDALYDVFVMKTRSDAWVIFGS
                     SSSTTSECRLIPADQPLAEPRLVEKRQEDVEYYLDHRGDTFYIRTNKDAKNFKVMTAP
                     VATPEAAHWKELVAHRPDIKIEDTDIFQDFWVTSERVKGLRHLRVTMFKDNTTHDIEF
                     PDAAYAVSPSGNAEFETARYRFSYQSPTTPSSVYDYDPVTRERTLLKQVEVEGGFNPA
                     NYATERVYAKAYDGAEIPIAIIYRKGTPMDGTSPLLLYGYGSYGISSEATFSVARLSL
                     MDRGVIWAIANIRGGGEMGEEWHDQGKTVLKRNTFTDFITAGEYLVAHKYTSPDRMAA
                     LGGSAGGLLIGAVANMRPDLFKCLVAKVPFVDVMNTMLDPTLPLTVGEYLEWGNPNEK
                     DPYYYIRSYCPYTNVTPQRYPAILVTAGLNDPRVGYWEGAKWVAKLRSMKLDTNPLLL
                     RVNMGAGHAGQSGRYDAWKETAFDYAFVLSQLGIAS"
     gene            complement(10954..12873)
                     /locus_tag="VF720_05650"
     CDS             complement(10954..12873)
                     /locus_tag="VF720_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878872.1"
                     /translation="MSANGAAAPGTTCTGASTRDNSHRCEFANLFMNQSEIGRSRLAL
                     GLALGVIALQVAGIFVSGPATWGFDHLAFLPTSLAWGIVAAAIGLVLLPAPVLDGWAS
                     RLGQPGRARIAAATVIVLSVVLFWFGRVREFFLSDSRLLMDGIADASRHFAADTFAVR
                     LQAFVHQLMSGVPSQSASFRLVSIGSGVVWLGALLYAIGTMTKDGRQRFLLAALVVTL
                     GMTKLFYGYPETSPLLAAAVALFVAAGVRASVSGAGEVLVGAAAIAAGATHLTGLLCM
                     PAMAVVLFPRWKARPISRVALLGAPLVLATLYAILLLGRETGTDAYGDYWSEFIPLAG
                     PLHAKQAYTMFSVPHLIDMTNDLFLLGPFVIVGVVLAVIGASRLFDEGTRGNSIDALL
                     AALLIPFLALSWTFNRKLGAARDWDLVASVAVPGTLLFGRWLAQETRSAAWLTRAGVA
                     LGAAGALHLGGWILVDTQAQRSLARFERLFGAGAAVSPFARSYAHEEVGATHLAVKRL
                     PEATHAYEEAVKADPGNAKAAGSLGAIYGSQGKTEEAMVLFRETVRLRPDIAIGHFNL
                     ANALARRNEWSDAEREYREALRLDPENGLAWLYLGSVIKRQGRAEEARSVWRDGLQHA
                     SPDVVRRIEEAIRAE"
     gene            complement(12815..13414)
                     /locus_tag="VF720_05655"
     CDS             complement(12815..13414)
                     /locus_tag="VF720_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7878873.1"
                     /translation="MERRLILIAALAAAGRFDEMAEAVRAAHDDGVSTAAIREAIVFL
                     VPYAGWPTALNALLAAHAVVPLSATTTDPLDTADRAARRELGLTTARTVNDRFDRVAE
                     NVAAIDPALVDLLAEGAYGYVYNRAGLDLVQRELLAVAILAVQGHDRQLLYHLRGAAN
                     VGASQDDITGTIEALADVSDDVRERGRRAWNDLYRRLNP"
     gene            complement(13399..13536)
                     /locus_tag="VF720_05660"
     CDS             complement(13399..13536)
                     /locus_tag="VF720_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878874.1"
                     /translation="MTDPALKKAIRAAAETEERQNPAPDARVPDIQRKPLEEIVQWNA
                     G"
     gene            complement(13533..13745)
                     /locus_tag="VF720_05665"
     CDS             complement(13533..13745)
                     /locus_tag="VF720_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878875.1"
                     /translation="MFTGLCASCRHHRWIVSGRGSRFLFCEWSKSDPAWPKYPPLPVM
                     ECSAYVRAAGSVGAADPLGESDGDRS"
     gene            13895..14113
                     /locus_tag="VF720_05670"
     CDS             13895..14113
                     /locus_tag="VF720_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878876.1"
                     /translation="MLSFEKDIKPMIREEDRNEMMFAFDLWNYAEFKRDLPRIADRIR
                     DKSMPPDGGWPDEWIAKFDQWIAEGMNP"
     gene            14086..>14864
                     /locus_tag="VF720_05675"
     CDS             14086..>14864
                     /locus_tag="VF720_05675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX7878877.1"
                     /translation="MDRRGDEPLKEPAGDVHVPALDGLRGLAILLVLAHHFNILNPAG
                     PAERNLILLLDLGKHGVDLFFVLSGFLITGILLDTRGRPGWLGSFFARRALRIVPLYY
                     ALLTALLVVQPLLLARNPATLSASMRWSAEAADAPWYWLFTSNILFAIREGFGLHGLD
                     VTWSLAIEEQFYLAWAFLVMFLDRRAMIVACTALIVLCPVIRLAIGLTGDSWLDAYLL
                     TPGRLDALAMGSLLAALYRAPDESMRRRLPHLAGWVAAVAIS"
BASE COUNT         2615 a         5083 c         4759 g         2407 t
ORIGIN      
        1 ggtgctggat gtccgtggcc gagcggtccg gacgctgcac gacggtgcgc tggagggggg
       61 cgaattcacc tggccgtgga gcggcgagga tgacgacggc cgcgacgtcc cgaacggggt
      121 ctacctggtg atcgtcgatg ccggatcgga ccggtcggcg aggaaaatcg tcctggcccg
      181 ctgatcccgg aaaccgggcg aagccggggg tgccgatgcc cccctataat gtaggcgggt
      241 tacgccaatt tcctgataat tgattgaatc acaataaatt ggaccgattc cgccccgggg
      301 ggtttccaag ttgacacccc ccggggcggt gcctatagtt tcggccttgt ctggagatct
      361 tccgtgaccc caccaaagcc cgccggcccg gccgacagtc ccgttgccgc cgccgacacc
      421 atgacgaccc catacgccga tatcttccgc agggcccaca ccttcaccaa ggcccaagaa
      481 gcccgtgcgt ccggtttcta tccctacttc atcccgattt cggggtccga tggcaccgag
      541 gtgaacatcg gggggcagaa ccggatcatg atcggctcca acaattacct cgggctcacg
      601 cacgatccgc gcgttctggc ggcggccgaa aaagccgccc gcgagtacgg ctcggggtgc
      661 accggcagcc ggttcctgaa cggcacgctc gatcttcacc tgaaactcga gcacgacctc
      721 gccgagttca tgggttacga ggcggcgctg gtcttctcca ccggcttcca gaccaacctc
      781 ggcacgatcg ccacgctggt caccaaggac gatgccgtca tcatcgacaa gctcgaccac
      841 gcctccatcg tggacggctg cttcatgtcg ccggggaaag tgttccgcta ccggcacaac
      901 gacatggagg acctcgagcg gcagttgaac cgaatcgaac cggagcgcgg ccgcctgatc
      961 gccgtggacg gcgtcttcag catggagggg gacatcgccc cgctgccgga gatcgtgcgg
     1021 atcgcccgga agtacggcgc ccgaatcctg tgcgatgacg cgcactcgct cggtgtcctc
     1081 ggatccaggg gagacggcac ccccgcccac ttcggcctca cgaaggaaat cgatttggtg
     1141 atgggcacct tctccaagtc gttcgcctcg atcggcggcg tcatcgccgg cgatgccatg
     1201 gtcatcgact acatcaagca cttcgcccgg ccgatgatct tctcggcggc gcttcccccg
     1261 tatgccgtgg ccaccgtgca cgagtgcctc aacatcatga aggccgagcc cgagcgccgc
     1321 gagcggctgt ggcagatcac ccgcaagatg ctcgacggct tcaagagcct cggcttcgac
     1381 acctgcacga gcgagacgcc gatcatcccg atcatgaccg gcgacaagga aaagaccttc
     1441 tatttctggc gatcgctctt cgaggccggc atcttcgcca acgcggtcat cccgccggcc
     1501 gtcccggaaa atgcctgccg catccgaacc tcgtacatgg caacccacac cgacgagcag
     1561 cttgatatcg tgctggagac cttcggaaaa atcggcaggc aggtcggcat catctgaccc
     1621 gaccggatgc ccccacctga gctgaccgtc catcccgtcg cgcccgggcg tgatttcagg
     1681 cgattcgcgc tcctgccgtg ggacatctac gccggcgacc cgtattgggt gccgcccctc
     1741 ctgatcgact tcaacaagct gttcgacacg gcgaagcatc ccttccatcg ccactccgaa
     1801 gcgcagccct tccttgccgt gcgcggcggg aagccggtgg gccgcatcac cgccatctgg
     1861 aaccggaacc accagaagtt ccacggcgag gacgcgggat tcttcggctt cttcgagtgc
     1921 gtcgacgacg ccgacgtggc cgccgcactg ctggaggtcg ccgcggcctg gctgaaggaa
     1981 cggggtctca ccgtgatgcg gggcccggcc aacttcagca gcaacgagga atgggggctt
     2041 ctggtcgacg gctggaacgg cacgccgaaa gtgatgatgc cgtacaaccc acgctggtat
     2101 cccgccctgt tcgacaaggc cggccttcgc acggccaagg acctggtggc ctactttctt
     2161 cctcacaacc agatcccgga acgcgtggcg cgcggcacgg cgctggtcag gcggcgctcg
     2221 cccgacgtca cgatccgctc gatcaacatg aagaagttcg aggccgaggt aggcatcatt
     2281 cgcgacatct acaacgcgag ctgggaaaag aactggggct tcgtgccgat gaccgacccg
     2341 gaaatcgagc acatggccag ggagctcaag cccgtggtcg atcccgacct ggtctttttc
     2401 gcggagaaag ccggcgaggt gatcggcttc tcgctctcgc tccccgacgt gtatcccgcc
     2461 ctgcagaagg ccaacggccg gctcctgccg ttcggcatcg tcaaactcct ctggtacgcg
     2521 cgccaggccg agtgggtgcg cgtcctcacg ctcgggctcc tgccggaaca ccggaaatcg
     2581 ggcgtggacg tcatgatgta cgaccacctc ttcaaggtgt gccagcgcaa gggttaccgc
     2641 ggcggcgagt tctcgtggct cctggaagac aacctcgcca tccgcacggc aatggacaac
     2701 ctgggcggtt acgtttaccg cacctatcgc ctgtacgaga aggagatcgc gtgaaggcgc
     2761 tcgtgaccgg cgccaccggc ttcatcggcg gccacctcgt ggaggcgttg ctcgatcgcg
     2821 gccacgaggt cgtggcccta gtgcgcgatc cggcgcggct cgggcccctg cgcgatcatc
     2881 cgagagtcac gtcaaccatc ggggacatca cgaaaccgga gacgatcaac gccgccagct
     2941 tcgacgtgga cgtcgtgtat caccttgccg ccgcgctccg cgcccgcaat gacgccgact
     3001 acgccgccat caacacccgc ggcaccgaag cgatcgcccg ggccgtggcg gccgcgccct
     3061 cacgccccag gctcgtgtac gtttcgagcc tggcggccgg tgggccttcg cccggcgatt
     3121 ccgtgctggt cggcaccgaa acgccccacc cggtgtccag ctacggccgc accaagctgg
     3181 ccggggaagc agtcatccgc aaggtgctgg gcaaggaaca tccgtggctc atgtttcggc
     3241 cgccgccggt ttacggcccg cgcgacaagg atgtcttcct gctgttcaag accgtgaacg
     3301 gcggcgtgct ccccatcccc ggtgacggtg ggcaggtgtt gccgatgctt cacgtggccg
     3361 acctggccga ggcgctggtc ctggccggca cgagcgagtc ggcgggccac acctggtaca
     3421 tcaccgacgg cggcctctac ccgttccgcg acgtgctttc ggctctcgcc cgggccctga
     3481 acaggaagcc gctccgcctc aacgtgccga tgtggatcct caggatcggc ggttccatcg
     3541 gccaatggct gtacgacacc acgggccgcc ccatggcact gaacaacgac aaggtgatcg
     3601 agatcgaggc gcctgcctgg cgctgcgacc cgacgccggc cttccgcgcc ctcaacttca
     3661 ccccccggtg gtccctcgac gaggggctgg cgcagacggc ccgctggtac cgggagaacg
     3721 gctggctgtg accccttcct ccacgatgcg cgatcccgcg cccggccgct atcgcctcat
     3781 cgacaaggtg gcgctcggct atttcgcgat cggtacgctg ctgctcgcgc atccgaaccg
     3841 cccggacaac tggctcacgc ttctactctt ccacgccatc tatcttgtcg gcatcccggc
     3901 ggccgtgcgc tggtcgaacc ggagcctgct cctctccctc ttccgggact ggtacccggt
     3961 cgtcggcatc atggccatgt acggcgagct gcagttcctg aaccgcgtcc tcaccgatcg
     4021 ctatttcgat ccggtggtca tggaatggga ggaggccatc ttcgggcggc agatggccgt
     4081 tcacctgcgg gagatgattc cctccaaact gttcggcgag gcggtgcact tcggctactt
     4141 ctgctactac ctcacgatcc cgtcgctcct gatcccgctg tggctcctca ggaagtaccg
     4201 ggagttccgg atcagcgtgt cggtgatcgg cgccgtctac atcgcctgtt acctctggta
     4261 cctcttctgg ccggtgacgg ggccctactg gcagctgccg aagcccgatc cctccaccga
     4321 ggggtggctc tttccgcaga tcaccaactt cttcgtggcc aacggctcct cacgcggctc
     4381 ggccttcccg agcagccacg tggccgcctc gatcacggtc ctggggatgg cgcggctcta
     4441 cctgaaaccg gtgtatcgca tcctctttct gccggtgatg ctgctgatca cggggacggt
     4501 gtacggcggc ttccattacg cgatcgacgc gatcgcgggg ctcgcgctcg gcctcacgtt
     4561 cgatatctgg gggccgaagg tcgtgacgaa ggtggacggg aaggcgatct gacgggcgtc
     4621 agcgcaatcc cgcgtcccag ttcttcatca cggtgaagcc ggggttggtg ttcttgatcg
     4681 cctggttgag caggaagcgt tcgccgtccg acgtcaccgc ccagcggttg accacccagc
     4741 cgcgggcatt caggtcggcg tggaacagga cctccggtgt gcccggctgg aattccgagc
     4801 ccggtgtcac gggcacggaa acgatgtcac tgttcgcgtt ctggtacagg agttcggttc
     4861 catggcgggc ccagtgtggc gtacttccgc cggcgttgga tacccgccac ttgcgtgccc
     4921 cgtccggaaa ggatcggacg tagagctcgc cccgccccgt ctcttcggag ccatacgcga
     4981 gccagcggcc atcgggcgag aaggccgcgc tggcctcgat ggcgggcgtt acgcagaacg
     5041 gccgcggagt ctcacggccc tggacgtccc ggatcacgat gttccagtcg tccatcgccg
     5101 tcgttcccac ggacatgagg acggcaagcc accggccgtc gggtgacaca ctcaatggcc
     5161 cttcatggcc gtagttccct accctgagcg tgtcctcctg ccccgggctg ttcacaagga
     5221 aggagtaggt caggtagttg ccgccctgtc ggtcggtgct gtagaaaatc ctcgttccgt
     5281 ccgcggacca gatgggcgag gtctcctgcg ccgggctgaa cgtgaagcgc gttgagatgc
     5341 cgcgatcgat gtcgcgcacc cagatgtcgt cgttacccga cgccgggtct tcgatcgaga
     5401 cggccagtcg tttcccatcg ggcgaaagat cgagctcgcg gtagcgatcg ggctcgccca
     5461 gcttccccag ccgccgcccg gtgcgatcgg tccagacaag ctcgctgatc aatcccgtta
     5521 cgttgaccag caccaccacg ccacccccgg ccgaatagat caggccattg gtgaacggcg
     5581 gcacatcctc cgccagggtc atgaactcac cggtcaaagc ctttcgccca aggtcgagcg
     5641 tccgggccac cagggtcgtg ccccggagaa agatcacgtg gttcggaggg tcgaaagcca
     5701 cgtatccatc gcacggaccc agctcttcgg cctcgagcga gccgagacgt cccagcatga
     5761 tggatgccgt gacggtgcca ccctccttcg agcgcgccgc aacaaagagg aaatgccggc
     5821 catcggggag gaacctggcg gaaaagtgat agaggtcgcc tcgcgcccga tcgaggcggg
     5881 aggcgggcgt catcgtgcca tccgcaacgg aaaccgccac gagcgagtcg cgcccggttg
     5941 gttccaggag gatggtcccg gccggactcc acgagcaggg caaccgaccg tgtacatcgg
     6001 cgatcggaac gacggcacct cccccgaccc cgatgcgcac gagcttgtca tcggcaacga
     6061 acgcgaggct ggcgtcgtcc ggcgaccaga tgggcgctcc ggccggggcg ctcgagacct
     6121 ggtgggcctc gatggaatcc agccttctca accagagccg ctgcacatcg ctggaatccc
     6181 acccgacaaa cgccagctgg cggccgtcat gggacacggc tggtccaccg aaggccgtga
     6241 gaccgggcgg cctcaccacg ctgaaatgta cggcttcccg ctcgacgcgc ggcttgtgga
     6301 gcgcgagcgt accggcaatc gtcaggccca ccagcgcgag tgcggcgccc gccgcccaca
     6361 gcgggttgag tccccgtcgg cgcgacacga ccccggcaga cacggcggac acaccgctcg
     6421 cctccccctc cacgatccag cgcagctgca gcttcacgtc gtgcgcgctc tggatccgct
     6481 cgtcccgatc cttctcgagg cacgccctca ccagtcgatc gaacgccgga ggcgtgagcg
     6541 gcgccatgcg cgacatcggc gcgggctcgc ccgccatgat ggccgagatc aggccggccc
     6601 ggctctgacc cgtgaacgcc cgctggccgg tggccatctc gtagagcacg caacccagcg
     6661 cccacaggtc gctccgcgcg tcggcttctt ttccttcgaa ctgctcgggc gccatgtact
     6721 ggaacgtgcc gacgatcatc cccttctcgg tcaccggctg cgccatcgtg ggggattggg
     6781 tggcggcggc cgacatcccg ggcgatgagt ccaggctcgc gcgtgcccgg gcgaggccga
     6841 agtccatgag cttggcgccg ctcctggcca gcatcacgtt tcccggcttc aggtcacgat
     6901 ggacgaggcc gcttcggtgg gcgcgatcga gtgcgtccgc gatctcgatc ccgatccgga
     6961 ggagttgcgg gatgggcaac ggcccccgcg cgaggcgatc ggccagcgtt tcgccgtcga
     7021 cccgttccat gaccaggaaa tcgatgccgt tctcacggcc gacatcgtga agcgtgcaga
     7081 tgttgggatg attgagcgag gagacggcgc gcgcctcccg ctcgaaccgg gcccgcgcct
     7141 ccgcggattc cttgaggtga tgcggcatca ccttgattgc gacgtcacgc tcgaggcgcg
     7201 tgtcgcgggc gaggtacacc tcgcccatcc ccccctcgcc gagcttggag gtgatctcgt
     7261 agggaccgag tctcgtgccc gcggccaggc tcatgggcgg aggatacttc cgaaactacc
     7321 cgggtggaaa cgccgggcga tgcctatccg gtgcgttcgc cccggtgccc ggcgtgttcg
     7381 ccggggtgcg gcggcgggag gccgatcgag gccagcgcaa tcaccacgcc ggcggccgcc
     7441 acgagccagc cgatgtcggc cggaattcgc gccaggaaat aggcccccgt caggccgccg
     7501 agtccggcgc cgaagaactg gcacatgctg tacgtccccg ccgccaggcc gcgcacctcc
     7561 cgcccggcgt acagcgtcac gaggctcggc aggatcggct cgaggacggc gatgccggtc
     7621 acgaccacga tgaggccgaa cgtgattccc ccgagtcgtt catagccgcc gcgcaggatc
     7681 agcgagccca ccacgaggag cagggccccg atcaccagga aaagccgctc ggcacggaat
     7741 cggtcgacga ggaacgcggt ggcggccagc gccgccacgc cgacgacgat ggccggcgcc
     7801 aggacgcgcc acatctgttc gcgcgggatc agtgtttcaa tctggatcgg caggaccacg
     7861 aagagggccg tgagcccgag gtgaagcgcg aacacggcca gattcacgcg cgccagcgtc
     7921 aggtcgtgca gcaccccgat cgtctgtcga aacgtggggc gtggcggatg aggcgcgtca
     7981 cggccgctcg gcacgaagac gaggatggcc acggccgaga gggcgcccat caccgcacag
     8041 atgtaaaaga gtcccggcac cgacagcttc acggccagga tcggacccag cgtgaggccc
     8101 aggccgaacg tcacgccgat cacgatgccg aggagcgaca tcgcccgctg gcggtattcg
     8161 gggcgggcca ggtctcccac cagcgccatg acggcggagg atatggcgcc ggcaccctga
     8221 agggtgcggc ccaggacgag aaggggcgcg gtctttgcca tcgcggccac caggctcccg
     8281 acaacaaaga gtaggaggcc cccgacgatc gtgcgccggc ggccgtagtg atcgctgaag
     8341 gcgccgaaag gaacctggaa ggcggtctgg gcgaagccgt acgaaccgac ggccattccc
     8401 acccagaacg tggcgccgcc ggggagtgat gcggcgtacg gcgagaggat cggcaggacg
     8461 aggtaaaggc ccagcatccg caatccgtac gcgaacgcaa ggctcaggag gccccaatgc
     8521 atcgcgctac gatagcaaag agagggtcac ctgtcggtgg aagcgccttc gcccgtctga
     8581 tacccttccg gctattccac ccggccggtt cgccctgtcc agcgtccaaa tcacggcggg
     8641 ccggccgcct ttgcatggaa tcgccgcgtc cattcgctcg ccggagaatc ccatgtcccc
     8701 acgccccgcg gttgcgttcg cgatcgccct caccctgagt tccctgcctc tttccggcgc
     8761 cttcgccgaa gacagcgact ccaataggat gatactgatg gatgacaaga atccgccggt
     8821 ggccaaggtc gagccgcacc cggtcacgct ccacggcgac acgcgcgtcg acaactattt
     8881 ctggctgcgc gacgacaagc gccaggatcc ccaggtcatc agctacctcg aggcggagaa
     8941 caattacacg gcccgggtga tgaagccgac cgaggcattg cagcagcgga tcttcgagga
     9001 gatcaagtcg cgcgtaaagg agacggacct cacggtgccg tatcgcgaga aagggtactt
     9061 ctattacagc cgcacggaag agggaaagca gtaccccatt ttctgccggc gggccggctc
     9121 gatcgacgga cccgaggaga aatactgcga cgagaacgag atggccaagg ggcatgacta
     9181 tttcagcctg ggcgtcctcg acgtgagccc cgacggaaag ttgatggcct actccaccga
     9241 caccgacggt tcggaaagtt acgttctccg cttcaggaac ctggcctccg gccaggaata
     9301 ccccgaatcg gtcaaggacg tggacggctc cgtctgggcc aacgacaacc gtcacgtctt
     9361 ctacaccacg cgggattccg ccaagcgctc ctaccgcctc tggcgccacg aactcggcac
     9421 gtcgcccgaa aaagacacca tgctctacca ggaagacgac gccctctacg acgtcttcgt
     9481 catgaagacc cgcagcgacg cctgggtcat cttcggctcg tcgagctcca ccaccagcga
     9541 atgccgcctg attccggccg accagcccct cgccgaaccc aggctggtcg agaagcgcca
     9601 ggaagacgtc gagtactacc tcgaccatcg cggcgacacg ttctacatcc gcaccaacaa
     9661 ggacgccaag aacttcaagg tgatgaccgc gccggtggcc acgcccgagg cggcgcactg
     9721 gaaggaactg gtggcgcacc gtcccgacat caagatcgag gacaccgaca tcttccagga
     9781 tttctgggtg acgtcggagc gcgtgaaggg tttgcgccac ctgcgcgtga ccatgttcaa
     9841 ggacaacacc acgcacgaca tcgaatttcc cgatgccgcc tacgccgtga gtccctccgg
     9901 caacgccgag ttcgagaccg cgcgctaccg cttcagctac cagtccccca ccacgccgag
     9961 ctcggtgtac gactatgatc cggtcacgcg cgagcgcacc ctgctcaagc aggtggaagt
    10021 ggagggtggt ttcaaccccg ccaactacgc caccgagcgc gtctacgcga aggcgtacga
    10081 cggtgcggag atcccgatcg ccatcattta caggaagggg acgcccatgg acggcacctc
    10141 tccccttctc ctgtacggct acggctcgta cggcatctcc agcgaagcca cgttttcggt
    10201 ggcccgcctc tccctcatgg accgcggcgt catctgggcc atcgccaata tccgtggcgg
    10261 cggcgagatg ggcgaggagt ggcacgacca ggggaagacg gtcttgaagc gcaacacctt
    10321 caccgacttc atcaccgccg gcgagtacct ggtggcccac aagtacacct cacccgaccg
    10381 catggccgcc ctgggcggct cggccggggg cctcctgatc ggtgccgtgg ccaacatgcg
    10441 ccccgacctc ttcaagtgcc tggtggccaa ggtgccgttc gtcgacgtca tgaataccat
    10501 gctcgacccg acgctgccgc tcaccgtcgg cgagtacctc gagtggggca accccaacga
    10561 gaaggacccc tactactaca ttcgctcgta ctgcccgtac accaacgtca cgccgcagcg
    10621 ctatcccgcc atcctggtca ccgcgggcct taacgacccg cgcgtcggct actgggaagg
    10681 cgccaagtgg gtggccaagc tccgctccat gaagctcgac accaatccgc tgttgctgcg
    10741 cgtgaacatg ggcgcgggac atgcggggca gtcggggcgg tatgacgcgt ggaaggagac
    10801 ggcgtttgac tacgcgttcg tgttgagcca gttggggatt gcgagttagc aaacctgagt
    10861 agaagccaat atcgcccgga gtgtccgcga cgctccgggc gattctgttt tcggttcagg
    10921 gttgaactcg gtttgaaccg agtcggatcg gggtcactcg gctcggatcg cctcctctat
    10981 ccttcgcacg acatcgggac tcgcgtgctg gagcccgtcg cgccacacgc tcctcgcctc
    11041 ttcggcgcgc ccctgccgtt tgatgaccga tccgaggtaa agccaggcca gtccgttctc
    11101 cgggtccagc cgcaaggcct cccgatactc ccgttcggcg tcgctccatt catttcgccg
    11161 cgccagtgcg ttggccaggt tgaagtggcc gatggcgatg tcgggcctca ggcggacggt
    11221 ctcacggaag agcaccatcg cttcctcggt ctttccctgc gatccgtaga tggcgccaag
    11281 cgaaccggcc gccttggcgt tgccgggatc ggccttcacg gcctcctcgt aggcgtgcgt
    11341 ggcttccggt agccgcttca ccgccaggtg tgtcgccccg acttcctcgt gtgcatagga
    11401 gcgcgcgaac ggcgacacgg cagcgcccgc tccgaagagg cgctcgaatc gcgccagcga
    11461 gcgttgggcc tgagtgtcga ccagtatcca accgcccaga tgcagggcac cggccgcacc
    11521 caaggccact ccggcgcggg tgagccaggc ggcactgcga gtctcctgtg ccaaccaccg
    11581 accgaacaat aacgtcccgg gcaccgccac cgacgccacc aggtcccaat cgcgcgcggc
    11641 accgagcttg cggttgaacg tccaggagag ggcgagaaac gggatgagga gtgccgcaag
    11701 caaggcgtcg atcgagttgc cgcgggtgcc ttcatcgaac agccggctcg caccgatcac
    11761 ggcgagcacc acgccaacga tgacaaaggg cccgagaagg aaaagatcat ttgtcatatc
    11821 gatgagatgc ggtacggaga acatcgtgta ggcctgtttg gcatgcagag gcccggccag
    11881 ggggatgaat tcggaccagt aatcgccgta ggcgtccgta cctgtttctc gaccgagaag
    11941 aaggatcgcg tagagcgttg cgaggaccaa cggcgcgccg aggagcgcca cgcgggaaat
    12001 ggggcgtgcc ttccagcgag ggaaaagtac gaccgccatc gcgggcatgc acagcaaacc
    12061 ggtgaggtgc gtggctcccg ccgcgatggc cgcagcccca accaacactt ctcccgcgcc
    12121 gctgaccgat gcccgcaccc ccgccgccac gaacagcgcc accgccgccg ccagcagagg
    12181 cgacgtctcc ggatacccgt agaacaattt cgtcataccc aatgtcacga cgagcgccgc
    12241 gaggagaaat ctctgtcgtc cgtccttcgt catcgtgccg atggcgtaga ggagggcgcc
    12301 gagccacacg acgccgcttc cgatcgacac gagccggaac gacgcggatt gcgacggcac
    12361 gcctgacatc agctgatgca cgaaggcctg gaggcgcacg gcaaacgtgt cggcggcgaa
    12421 gtgccgcgag gcatcggcga tgccgtccat cagcaggcgg ctgtcggaga ggaagaactc
    12481 gcggacgcgg ccgaaccaga agaggaccac cgacaggacg atgaccgtcg cggcggcgat
    12541 gcgggcccgg cccggctgac ctagccgcga agcccacccg tcgagaacgg gggccggcaa
    12601 cagtacgagc ccgatcgccg cggccactat cccccacgcc agtgacgttg ggaggaacgc
    12661 cagatggtcg aatccccacg tggcgggccc ggacacgaag attccggcca cctgcagcgc
    12721 aatgaccccg agcgccagcc cgagcgcgag tcggctccgg ccgatctccg actgattcat
    12781 gaacaggttg gcgaactcgc accggtgcga gttgtcacgg gttgaggcgc cggtacaggt
    12841 cgttccaggc gcggcggccc cgttcgcgga catcgtcgga aacatccgcg agggcttcga
    12901 tggtaccggt gatgtcgtcc tggctggcgc cgacgtttgc ggcgccccgc aggtggtaca
    12961 gaagctgccg gtcgtgcccc tgcacggcca ggatggccac cgccaggagt tcgcgctgca
    13021 ccaggtcaag gccggcgcgg ttgtacacgt agccgtaggc gccttcggcc agcaggtcca
    13081 ccagcgccgg gtctatggcg gccacgtttt cggccacgcg gtcgaaacgg tcgttgacgg
    13141 tgcgcgcggt ggtgaggccc agttcgcgac gggcggcgcg gtctgcggtg tcgagcgggt
    13201 cggtggtggt ggccgagagc ggaacgacgg cgtgggcggc gagcagggcg ttgagtgccg
    13261 tgggccagcc ggcgtacggc accaggaaga cgatggcttc gcggatggcg gcggtggaaa
    13321 cgccgtcgtc atgggcggcc cggacggcct cggccatctc gtcgaaacga cccgcagcag
    13381 ccagggcggc aatgaggatc agccggcgtt ccactggacg atctcctcga gcggcttgcg
    13441 ttggatgtcc ggcacgcggg cgtcgggcgc cgggttctgc cgctcttcgg tttcggcggc
    13501 cgcgcggatg gccttcttca gcgccgggtc ggtcatgaac gatctccatc gctctcgccc
    13561 agcggatcgg ccgccccgac ggacccggcg gcacgcacgt acgccgaaca ctccatgacc
    13621 ggcagcggcg ggtacttggg ccaggcggga tcgctcttgg accactcgca gaagagaaac
    13681 cggcttcccc gtccggagac gatccagcga tggtggcggc acgatgcgca gaggccggtg
    13741 aacatgccgg gcgatcttac cgccgggccc tgcggggcac cagacggccc gggcggcccc
    13801 ggccgcccgg agcgcatgct acaatcccgc cgcttttggc cggcacggcg ccgtgacgcg
    13861 tgtgcggccc caccacagcc ttggaggagc gggcgtgctc agtttcgaga aagacatcaa
    13921 gccgatgatc cgtgaagaag accggaacga aatgatgttc gcgttcgacc tctggaatta
    13981 cgccgagttc aagcgcgacc tcccgcgtat cgccgaccgg atccgcgaca agtcgatgcc
    14041 gccggacggt ggctggcccg atgaatggat cgccaagttc gaccagtgga tcgccgaggg
    14101 gatgaacccc tgaaggagcc ggccggggac gtccacgtcc cggcgctcga cggacttcgc
    14161 ggcctggcca tcctccttgt cctggcacac cacttcaaca tcctgaaccc ggccggcccc
    14221 gccgagcgga acctcatcct gcttctcgac ctcgggaagc acggcgtgga tctcttcttc
    14281 gtcctgtcgg gcttcctgat caccggcatc ctgctggata cgcgcgggcg accggggtgg
    14341 ctcggcagtt tcttcgcccg gcgggcactc cgcatcgtgc ccctctatta cgccctgctc
    14401 acggcgttgc tcgtcgtgca accgctcctg ctcgctcgca atccggccac cctgtccgca
    14461 tcgatgcgct ggagcgccga ggcggccgac gccccgtggt actggctctt cacctcgaac
    14521 atcctcttcg ccatccggga agggttcggc ctccatgggc tggatgtcac ctggagcctg
    14581 gccatcgagg agcagttcta cctcgcatgg gccttcctgg tgatgttcct cgatcgtcgc
    14641 gccatgatcg tggcctgtac ggcgctgatc gtgctttgcc ccgtcattcg cctggcgatc
    14701 ggcctgaccg gtgacagctg gctcgacgcc tatctcctca cgcccggacg gctcgacgca
    14761 ctggcgatgg gctcgctcct cgccgcgctc taccgcgcac ccgacgagtc catgcgcagg
    14821 agacttccgc acctggccgg ctgggtggcc gccgtggcga tttc
//
LOCUS       DASVOD010000262         5725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_226799, whole genome shotgun sequence.
ACCESSION   DASVOD010000262 DASVOD010000000
VERSION     DASVOD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5725
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226799"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..934
                     /locus_tag="VF720_05680"
     CDS             341..934
                     /locus_tag="VF720_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878878.1"
                     /translation="MSIMAQLDLFTQPDQRPAERWALTRPMAASRLKQHAIARLDFVR
                     RHFPELDAVTVKVGRTTSRRAHAWASLDPAMPAIWIKPGALPLFTVTHELTHLLQARG
                     LAPRGEKAADLYALARHPELIDKPPTYLRVPRILFGFKGNPRPGTAMLLCRTARRAIA
                     EAPTKPRRAVRLFEEYAALAAVPSALRIYDMFGPMLA"
     gene            931..1440
                     /gene="sixA"
                     /locus_tag="VF720_05685"
     CDS             931..1440
                     /gene="sixA"
                     /locus_tag="VF720_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HEX7878879.1"
                     /translation="MSRHLYLLRHGEADHEGAPTWTHDSRRPLTRQGKEKMALQAAAM
                     QRTGLEVEAIVTSPYLRARQTAEIVAAAYRMEDRLVESDLLEPGASFNDLQKTLRTVS
                     AARVLVVGHAPDLGEWAGELTGSDPIALGKGWLAWIRLEGEMENGGGRLKALFPAEWM
                     QSVGRLLAG"
     gene            complement(1461..1985)
                     /locus_tag="VF720_05690"
     CDS             complement(1461..1985)
                     /locus_tag="VF720_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878880.1"
                     /translation="MNPRTRKVLLVLGAMWGIPLVAMLAAGLWVKSLGMVSVHVAEHR
                     PGGTNVDIQVPGVLASAGLMFLPDVVCAEVAEDVDSHWGEVVKAVEQQLRNCPDAVFV
                     QVENERENVSIRKEGKHLVIDVDSDDETVHLKVPFHLVSTVLSKIDGNPDAIRIRNVE
                     GRRGRNVTRIVTGA"
     gene            complement(1982..2554)
                     /locus_tag="VF720_05695"
     CDS             complement(1982..2554)
                     /locus_tag="VF720_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878881.1"
                     /translation="MRSNLLAAAALLVCLAPVAGASDTGRWLHIRVDESGPGGEKVRV
                     NVPLSMVETLLPLIDDDDFSGGRIRLNDHDLDAVKLRAIWKAVREAGEGEFITVEGDD
                     ENVRVSRSGQYLHVNVDETDEDGDDVKVRIPTMVMDALLSGSGDELDLVAAIRALGDD
                     GDGELVRVDSDDENVRIWVDASNSGEGSTR"
     gene            complement(2679..3074)
                     /locus_tag="VF720_05700"
     CDS             complement(2679..3074)
                     /locus_tag="VF720_05700"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX7878882.1"
                     /translation="MFKTEPSDFSYDDLEKKKESGWDGVRNAQALIHLRQVAAGDDVL
                     IYHTGSEKSVIGLARATGPARPDPKDTAGKMVIVPVVPVRRLPRSVPIAVIRGEKALA
                     EFPLIKNTRLSVMPVKPAEFRAILALAGA"
     gene            3138..4295
                     /locus_tag="VF720_05705"
     CDS             3138..4295
                     /locus_tag="VF720_05705"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7878883.1"
                     /translation="MKFAIIGSGRQGTAAAWDLAQFGDAQEIRLIDADTAVAEEAASR
                     INRLAGRNVAVARTVDVRDGAALTSALLGVNAALSGVPYFFNVDAAKAAITAGACFND
                     LGGNTDVVRAELALDAEARGAGISIVPDCGLAPGMGNTLAVHGMDQFDRPLSARIFCG
                     GLPQHPKPPLGYKLVFSLEGLTNEYTGEAIVLKSGRRVTEPAFSGRETIVVDGLGSLD
                     AFYTSGGTSTCPWTLEGKIPDYEYKTLRYPGHYDQLKPLIDLGFLDTKPVRVGDMEVA
                     PRQLAHRILERWIDFPEDHDLVVLRVDVTGEVNGKKRTYRQEIVDRQDPVTGFTAMER
                     TTAYPAAIVTILQARKEVPAGALPLESAVPGASFVRELNRRDIRLEAGWLD"
     gene            complement(4314..4559)
                     /locus_tag="VF720_05710"
     CDS             complement(4314..4559)
                     /locus_tag="VF720_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878884.1"
                     /translation="MQPITPIQIRRILEVTDALGIHREGVVVPLAREGTGAIERDGEK
                     VVITAPEGEVFELWLIGLAETLGESDLSGVPRLDPDE"
     gene            4696..>5725
                     /locus_tag="VF720_05715"
     CDS             4696..>5725
                     /locus_tag="VF720_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX7878885.1"
                     /translation="MNVTQLKTLVRGEVITDEAGLEARSSDFGRMIHRKPGAVVRPAD
                     TADVAAVLKFANENGYAVATRGEAHSQTGQSLTDGGIVLDLTSLNNILSVDREGLTVT
                     CQSGVVWRNLVDHLKPIGLIPRVLTNNLGVTVGGTLSVAGLGVASFRYGTQGDNAIEL
                     EVVTGKGDVVVCSATENADLFNGARSGLGQFAVITRATMKVRTFKPMVRRYFFLYDDL
                     DKFMADARMLMDTDRCDFLESWCVAAPMGFRTVGNEKQTFGEWFFPLHLTVEFDPSAP
                     PDDDDVIKGLSYYRFTHVEDEPIHDFANRLEPLFVLWKLAGYWANKHPWMETVLPWDS
                     GKEYIRNVL"
BASE COUNT          966 a         1990 c         1829 g          940 t
ORIGIN      
        1 gcggaacgtg tcgccctggc caacgtcctc gtgccaccag agccagtcga cccagaacgt
       61 cgtcccccgt ccgaagagcc agatcacacc gaacagcgcc agaagaaccg cgatgattcc
      121 cgtcggcggg cccgaagtgc gtccgggtcg aattgcccgc gcgttcatcg gccctcccgg
      181 gacaggcgac gcgcacttgg aaacgtcttc acggaagctc tccccatcgc gaacgggtca
      241 cggaaccgga ggccgagagt cgtggcgcat cgtccggccg gcgggagaat atcagcgatc
      301 gtgattgacg gtagcggggc tgtcccggca gactccgggc atgtccatca tggcccagct
      361 cgatctgttc acccagcccg accaacgccc cgcggaacgc tgggcgctga cgcgcccgat
      421 ggccgcctca cgcctcaagc agcatgccat cgcccgcctc gatttcgtgc gccggcactt
      481 cccggagctc gatgccgtga ccgtgaaggt aggacgcacc acgagccgcc gcgcccatgc
      541 ctgggcctcg ctcgatccgg ccatgcccgc aatctggatc aagcccggtg cactcccgct
      601 cttcaccgta acgcacgaac tcacgcactt gttgcaggcc cgcggactcg ccccgcgggg
      661 agagaaggcg gccgaccttt atgcgctggc ccggcatccc gagctgatcg acaagccgcc
      721 gacctatctc cgcgtgccgc gcatcctctt cggcttcaag ggaaacccgc gcccgggcac
      781 ggcgatgctc ctctgccgca ccgcccgccg agccatcgcc gaggcgccga cgaaaccgcg
      841 ccgggccgtt cgcctcttcg aggagtatgc cgcgctggcg gcggtcccgt cggcgctccg
      901 gatctacgac atgttcggcc cgatgctggc atgagccgtc atctctacct gttgcgacac
      961 ggtgaagcgg accatgaagg cgcgccgacc tggacccacg actccaggcg cccgctcacc
     1021 cggcagggaa aggaaaagat ggcgcttcag gctgccgcca tgcagcgcac gggcctcgaa
     1081 gtcgaggcca tcgtcacgag cccctacctc cgggcacggc agaccgccga gatcgtggcc
     1141 gccgcgtacc ggatggaaga ccgcctggtc gaatcggacc tgctcgagcc cggtgcctcg
     1201 ttcaacgatc tccagaagac gctccgcact gtctccgcgg ctcgcgtcct ggtggtcggg
     1261 cacgcacccg acctcggcga gtgggcaggc gagctgacgg gcagcgaccc gatcgccctc
     1321 ggcaaggggt ggctggcctg gatccggctc gagggagaaa tggaaaacgg cggggggcgc
     1381 ctgaaggcgc tcttccccgc cgaatggatg caatcggtcg gtcgtctgct ggccggctga
     1441 tcgcgccggc ccggcgccgc tcaggcgccg gtcacgatcc gggtcacgtt ccgcccccga
     1501 cggccttcga cattccggat ccggatcgcg tccgggtttc catcgatctt cgagagcacg
     1561 gtcgagacga gatgaaacgg caccttgagg tggaccgtct cgtcatcgga atcaacgtcg
     1621 atgacgagat gcttcccttc cttgcggatc gacacgtttt cccgttcgtt ttccacctgc
     1681 acgaacacgg cgtccgggca attgcggagc tgttgctcga cagccttgac gacctcaccc
     1741 cagtggctgt ccacgtcctc ggccacctcc gcacagacca cgtccggaag gaacatgaga
     1801 ccggcgctgg ccaggacgcc cggcacctgg atgtcgacat tcgtgccgcc cggccggtgc
     1861 tcggccacgt gaaccgacac catcccgagc gacttcaccc acaagcccgc cgcgagcatg
     1921 gccacgagcg ggatccccca catggcgccc agcacgagca ggaccttcct ggtacgcgga
     1981 ttcacctggt actcccctca ccggagttgc tggcatcgac ccagatccgg acgttttcgt
     2041 cgtcgctgtc aacgcggacc aattcaccgt cgccgtcatc accgagggcc cggatcgccg
     2101 cgacgaggtc gagctcatcg ccgctgccgg agaggagggc gtccatcacc atggtcggga
     2161 tgcggacctt gacgtcatcg ccgtcttcgt ccgtttcgtc cacgttcacg tggaggtact
     2221 gccccgaccg ggaaacgcgg acgttttcat cgtccccttc cacggtgatg aactcgcctt
     2281 ccccggcctc tcgtacggcc ttccagatgg cgcggagttt gacggcgtcc aggtcgtgat
     2341 cgttcaaccg tatccgcccg ccggagaaat catcgtcatc gatcaggggg agaagggtct
     2401 cgaccatgga gaggggaaca ttcacccgga ccttctcgcc gccggggccg gattcgtcca
     2461 cccggatgtg gagccagcgg ccggtgtcgg aagcgccggc gacgggggca aggcacacga
     2521 gcagggcggc agccgccaac aggttggagc gcatggaatt acctccgtct gtctgggtgg
     2581 gggcggcccg gcctcgtcgg gccgggctcg cggtcgtggg gttccagacg gcggagggag
     2641 ctgtcgggtt gccgaatgtc gggggaattc gcgccgggtc aggccccggc cagcgccagg
     2701 atcgcgcgga attcggccgg cttgaccggc atgaccgaga gccgggtgtt cttgatgagg
     2761 ggaaactcgg ccagggcctt ttcgccccgg atgacggcaa ttgggaccga cctggggagc
     2821 cggcgcaccg ggacgaccgg gacgatcacc atctttccgg cggtgtcctt cgggtcgggg
     2881 cgcgcgggac cggtggcccg ggccaggccg atcaccgact tctccgatcc ggtgtggtag
     2941 atgaggacgt cgtcccccgc cgccacctgc cggagatgga tcagcgcctg ggcgttgcgc
     3001 acgccgtccc atcccgattc cttcttcttc tcgaggtcgt cgtacgagaa atcggacggc
     3061 tcggtcttga acatccagtg cgccatggcg ggcgatgata gccgaaggtg cggggcctgt
     3121 ggcacactcc ccgccctatg aaattcgcca tcatcggctc aggccgacag ggaaccgccg
     3181 cggcctggga cctcgcgcaa ttcggcgacg cacaggaaat ccgcctgatc gatgccgaca
     3241 ccgcggtcgc ggaagaggcc gcgagccgca tcaaccgact ggccggtcgc aacgtggcgg
     3301 tggcccggac ggtcgacgtg cgggatggcg cggcgctcac cagcgccctg ctcggcgtga
     3361 acgccgccct ttcgggcgtg ccctacttct tcaacgtgga cgcggcgaaa gcggccatca
     3421 cggcaggtgc ctgcttcaac gacctcggtg gcaacaccga cgtcgtgcga gctgagctgg
     3481 ccctcgatgc ggaggcaaga ggggccggca tctcgatcgt gccggactgc ggcctcgccc
     3541 ccggaatggg aaacacgctg gcggtgcatg gcatggacca gttcgaccgg cccctttcgg
     3601 cccgcatctt ctgcggcggg ctgccgcaac atccgaagcc gccgctcggc tacaagctcg
     3661 tcttcagcct cgaaggactc accaatgagt acaccgggga ggcgatcgtg ctgaagtccg
     3721 gtcggcgagt caccgaaccc gctttcagcg gtcgcgaaac gatcgtggtc gacgggctcg
     3781 gctccctcga cgcgttctac acctcgggcg gcacctccac ctgcccgtgg accctcgagg
     3841 gaaagatccc cgactacgag tacaagacgc tccgctatcc cggtcactac gaccagctga
     3901 agcccctcat cgatcttggc ttcctcgata cgaagccggt cagggtgggc gatatggagg
     3961 tggccccgcg ccagctggcc caccggatac tcgaacgctg gatcgacttc ccggaagatc
     4021 acgacctggt cgtcctgcgc gtggatgtca ccggcgaggt aaacggcaag aagcggacgt
     4081 atcgccagga gatcgtcgat cgccaggacc cggtgaccgg attcacggcc atggaacgga
     4141 ccacggccta tccggccgcc atcgtcacga tcctccaggc ccgcaaggaa gtcccggcgg
     4201 gtgcccttcc cctcgagtcg gcggtgccgg gcgcctcgtt cgtgcgggag ttgaatcgac
     4261 gcgacatccg gctcgaggcc ggctggttgg actgacgacg cgcggacgac ccgttactcg
     4321 tccggatcga gccgcggcac tccggacagg tcgctctccc cgagcgtttc ggccaggccg
     4381 atcaaccaca actcgaaaac ctccccctcc ggggcggtga tcaccacttt ctcgccgtcc
     4441 cgttcgatgg ctccggtccc ctcgcgtgcc agcgggacga cgacgccctc ccggtgaatc
     4501 ccgagggcgt cggtcacttc gaggatgcgg cggatctgga tgggggtgat gggctgcatc
     4561 tggctcctcg ttggctgggc tgagttcgat ggagaaccgg caatcgaatc ggcggctcgg
     4621 ggatccgacc ggccggccga aacccttgat ataatacccg tccttttgaa ggtcctgtcc
     4681 cgcgcgaggt accccgtgaa cgtcacccaa ttgaagaccc tggtcagggg cgaagtgatt
     4741 accgacgagg ccggcctcga ggcccgctcg tccgacttcg gccgcatgat ccaccggaag
     4801 cccggcgcgg tggtccgccc ggccgacacg gccgacgtgg ccgccgtcct caagttcgcc
     4861 aacgagaacg gctacgccgt ggccacccgc ggcgaagccc actcccagac cggccagtcg
     4921 ctgaccgatg gaggaatcgt cctcgacctc acgtcgctga acaacatcct gtcggtcgac
     4981 cgcgagggac ttaccgtcac ctgccagtcg ggagtcgtct ggcgcaacct ggtcgatcac
     5041 ctgaagccga tcggccttat cccgcgcgtg ctcaccaaca acctcggcgt taccgtcggc
     5101 ggtacgctgt cggtggccgg cctcggcgtg gcctcgttcc ggtacggcac gcagggtgac
     5161 aacgccatcg agctcgaggt cgtcaccggc aagggtgatg tcgtcgtctg ctccgccacc
     5221 gagaacgccg acctcttcaa cggtgcccgt tccgggctcg gccagttcgc cgtgatcacc
     5281 cgggccacga tgaaggtgcg cacgttcaag ccgatggtcc ggcgctactt cttcctgtac
     5341 gacgacctcg acaaattcat ggccgacgcc cgcatgctca tggacacgga tcggtgcgac
     5401 ttcctcgaat catggtgtgt ggccgctccc atgggcttcc gaaccgtcgg caatgaaaag
     5461 cagaccttcg gcgagtggtt cttcccgctg cacctcacgg tggagttcga tccgtccgcg
     5521 ccaccggacg acgatgacgt catcaagggg ctctcgtact accgcttcac gcacgtcgag
     5581 gacgaaccga tacatgactt cgccaatcgg ctggagcctc tcttcgtcct ctggaagctg
     5641 gccggttact gggccaacaa gcacccatgg atggaaaccg tcctcccgtg ggattccggc
     5701 aaggagtaca tccggaacgt cctgg
//
LOCUS       DASVOD010000263         4770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_226808, whole genome shotgun sequence.
ACCESSION   DASVOD010000263 DASVOD010000000
VERSION     DASVOD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226808"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /gene="rpsP"
                     /locus_tag="VF720_05720"
     CDS             complement(<1..207)
                     /gene="rpsP"
                     /locus_tag="VF720_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX7878886.1"
                     /translation="MSVKIRMKRGGTTNRPFYRVVVIDSRMQRDGRFIEELGFYDPVQ
                     KPLVFNVDRDRVDYWMSKGAQPSDT"
     gene            complement(255..1583)
                     /gene="ffh"
                     /locus_tag="VF720_05725"
     CDS             complement(255..1583)
                     /gene="ffh"
                     /locus_tag="VF720_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX7878887.1"
                     /translation="MFEDLSGKLDDIFRRLRGKGKLTEDNIKETLREVRRALLEADVN
                     YKVAKDFVDQVRDKAIGQDVLKSLTPGQLVVKLVHDQLVELLGGASAPLARAPKPPTV
                     IMVVGLQGSGKTTFCGKLATWFKKKNQRVLMAACDIYRPAAIDQLETVGKQAGVPVYS
                     ERGAKPIQIVKGALDAAYKDVSDVLIIDTAGRLHIDDSLMAELGELKSAYKPNEILLV
                     VDGMTGQDAVTISQTFQERLGFDGVVLTKMDGDARGGAALSIRSVTGKPIKFVGTGEK
                     LEALEEFHPDRLASRILGMGDIVSLVERAQEAVDFDEAARLEEKLRREEFTFEDFLTQ
                     MQQMKRMGPMEDLLKMLPGVGNKLQGMTFDDKALGKIEAIIRSMTRDERHRPQIIDGS
                     RRRRIALGSGSSVQDVNRLLKEFEGMQKMVKMFGKMGKSGRKMAMPFPPR"
     gene            1962..2705
                     /locus_tag="VF720_05730"
     CDS             1962..2705
                     /locus_tag="VF720_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878888.1"
                     /translation="MTATQLGIALRTYLLPLPWTRAFRFSVLGVAVVYLVAMLIRMIG
                     RQHSGEEGELSFEMARALLMPGVPVFALLVSELPLREGLRHRTLLYPLLGPASRDAIA
                     LARAGATAVLFGLVFAVVVAGVAFIGGVPADRLGSPLLATFLGGMAYVSIFGFLQLLV
                     PRGLAAGMLWYFLLDEPIAKIPFELCRLSVAYHVRVLGHDHEFFGIPVSNFPAEQPTV
                     SVLFLLGVTIVALLATVALFRRRNLEELC"
     gene            2702..3640
                     /locus_tag="VF720_05735"
     CDS             2702..3640
                     /locus_tag="VF720_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878889.1"
                     /translation="MNPPWIEVSDLHLSRGGRPVLMGITAALHGRAVGLVGANGAGKS
                     TFIGALLGILAPDKGKVTVLGLEQPAAALEIRTRAGVMAEQAGIFPGGSGVDAVVFAG
                     MLSGLIRKEALRRAHRALDALDVGEERYRPTSEYSTGMRQRCKMAMSLVHDPEVLILD
                     EPTVGLDPPGRTQLLELIRGLRDEGRRILISTHILADAEFLCDEMLLLDAGTVAFSGP
                     VAAMIKGREERFVASGDRLDATFVARLAQSGIPAMIDDAGRLVLSATDAESIRRFWSV
                     ASECGAAIRRLDREAPTLEEALLARLDLVRGNGGAR"
     gene            3637..4422
                     /locus_tag="VF720_05740"
     CDS             3637..4422
                     /locus_tag="VF720_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878890.1"
                     /translation="MSAIPSAGHVITTIAQREFRIALRRRFLRTILILGFLPPIGAAV
                     VLIIRLLIKQMQGNDVGWDPLPFLLNFQAIPVFLAAIGLGTPAVSRDRAEDVLYLYAV
                     RPVNPALYAAGKMLAVFLPALLVMLLPIAMVITVRQGILGSDAAPLDSLADLGRALVL
                     TIGLAAAMAGATVGPSAATKRGRWALLLSLTLMTLPDTILRVFNLDGDWAIGPLNVSH
                     RLVETLFVSHAWMESLGHMAILLTWGLAGFLLTRWRVAREMIP"
     gene            4419..>4770
                     /locus_tag="VF720_05745"
     CDS             4419..>4770
                     /locus_tag="VF720_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7878891.1"
                     /translation="MTPVRLDAVSVFYGEVVGLSHVSLGLEPGLTGVVGPNGSGKSTL
                     MRVLTGLVSPREGSVNVFGGDPFASAEIRSRIAFVPATENFHDGLSGRDNLRLLATAM
                     GLTGGAVGARADKAL"
BASE COUNT          717 a         1660 c         1468 g          925 t
ORIGIN      
        1 cgtgtccgag ggctgggccc ccttcgacat ccagtagtcg acgcggtcgc gatcgacatt
       61 gaagaccagc ggcttctgga cgggatcgta gaagccgagc tcctcgatga accgaccgtc
      121 gcgctgcatc cgcgagtcga tgaccaccac gcggtagaag ggacggttgg tagtccctcc
      181 gcgcttcatc cgaatcttga ccgacaaatc atcctccttc gttcaccatt ggtttcgatc
      241 ggggtatcaa actgtcatcg gggcgggaac ggcatggcca tcttgcgccc gctctttccc
      301 atcttcccga acattttcac catcttctgc atcccctcga actccttgag gagacggttc
      361 acgtcctgca ccgaactgcc actccccagg gcgatccgcc gacggcggct gccgtcgatg
      421 atctgggggc ggtggcgttc atcgcgcgtc atcgaccgga tgatcgcctc gatcttcccc
      481 agggccttgt cgtcgaacgt catcccctgg agcttgtttc ccaccccggg cagcatcttc
      541 agcaggtctt ccatcggacc catgcgcttc atctgctgca tctgcgtgag gaagtcctcg
      601 aacgtgaact cctcccgccg gagcttttcc tcgagcctgg ccgcctcgtc gaaatcgacg
      661 gcctcctggg ccctttcgac gaggctcacg atgtcgccca tgccgaggat ccggctggcc
      721 agccggtccg ggtggaactc ctcgagagcc tcgagttttt cccccgtgcc gacgaacttg
      781 atgggctttc ccgtgaccga ccggatggag agcgccgcgc caccgcgggc atcgccgtcc
      841 atcttggtca gcaccacgcc gtcgaaaccg agccgttcct ggaaggtctg gctgatcgtg
      901 acggcgtcct gcccggtcat tccatccacg accagcagga tttcgttcgg tttgtaggcc
      961 gacttgagct cgcccagttc ggccatcagc gaatcgtcga tgtgcagacg acccgccgta
     1021 tcgatgatga gaacgtcgga cacgtccttg taagccgcgt ccagcgctcc tttgacgatc
     1081 tgaatcggtt tggcgccccg ctcggagtaa accggcacgc cggcctgctt cccaacggtt
     1141 tcgagctgat cgatggccgc cggacgatag atatcgcacg ccgccatcag gacccgctga
     1201 ttcttcttct tgaaccaggt ggccagcttg ccgcagaagg tggtcttgcc cgacccctgc
     1261 agccccacga ccatgatgac ggtgggcggt ttcggcgccc gagcgagcgg tgccgaagcc
     1321 ccccccagga gctcgacgag ctggtcatga accagcttga cgacgagctg ccccggcgtg
     1381 agcgatttga gtacatcctg cccgatggcc ttgtcgcgaa cctggtcgac gaagtccttc
     1441 gccaccttgt agttgacgtc ggcttcgagg agggcccgcc gcacctcgcg cagcgtttcc
     1501 ttgatattgt cttcggtcag cttccccttg ccgcgaagcc gccgaaaaat gtcgtccagt
     1561 tttccggaga ggtcctcgaa catggcttcc aatcacgggg agggccaatg acaaagggtg
     1621 aggagtatac gttttccgcg cgacccgggg caagaagagg atttgggggg cgcaaggacg
     1681 caaaattgca tctaagttat ttaattgtaa ttggttatca cagattttgg gagattttcg
     1741 tcctcgccga gaccactata tagagagtgc ccccggacaa gcacccggcg ggatccaacg
     1801 acgtgagatg cgatgttggc cggcgttccg ccggccatcc catggtcctg cgacatgccg
     1861 accaaggacg tcgaagatgt cgatgcggac ctcggcaccc ggtcgtccga tcatccccaa
     1921 tcgatgcaac gcccgggcat cacttcgcgt agcctccccg catgaccgcc acccagctcg
     1981 gcattgcgct gcggacctac ctccttcccc ttccatggac ccgggctttc cgcttttcgg
     2041 tgctgggcgt ggcagtcgtc tacctggtgg cgatgctcat ccggatgatc ggccggcagc
     2101 actcgggcga agagggagag ctctcgttcg agatggccag ggcgctcctg atgccgggtg
     2161 tgccggtctt cgcgcttctc gtgtccgagc tgccgctccg cgaggggctc cggcatcgca
     2221 cgctcctcta tcccctgctc ggtccggctt cgcgcgatgc catcgcgctg gcgcgcgccg
     2281 gagccaccgc cgtgctcttc ggcctcgtct tcgcggtggt ggtggccggc gtggcgttca
     2341 tcggcggtgt gccggccgat cggctcgggt cgccgctgtt ggccacgttt ctcggcggca
     2401 tggcctacgt ttcgatcttc ggtttcctgc aactcctcgt gccgcgcggc ctggcggcag
     2461 gaatgctctg gtacttcctg ctcgacgagc cgatcgccaa gatcccgttc gagctctgcc
     2521 ggctctcggt ggcctaccac gtgcgcgtgc tcggccacga tcatgaattc ttcggaatcc
     2581 cggtgagcaa cttccccgcc gagcagccga ccgtgtcggt cctcttcctg ctcggcgtca
     2641 ccatcgtggc gctcctcgcc accgtggcgc tcttccgccg ccgcaatctc gaggaactct
     2701 gttgaacccg ccctggatcg aggtttccga cctccacctg agccgcggtg gccgccccgt
     2761 cctcatgggg ataaccgcgg cgctgcacgg gcgcgcggtg gggctggtcg gcgccaacgg
     2821 cgcgggaaag agcacgttca tcggcgccct gctcggcatc ctggcccccg acaagggaaa
     2881 ggtgaccgtg ctcggccttg agcagcccgc cgccgcgctc gagatccgca cccgcgccgg
     2941 cgtcatggcg gaacaggcgg gcatcttccc gggcggctcg ggtgtggacg cggtggtctt
     3001 cgcgggaatg ctgtccggac tcatccgcaa ggaagcgctg cgccgggccc atcgcgcgct
     3061 cgatgcactc gacgtgggcg aggaacgcta tcgccccacc tcggagtact ccaccgggat
     3121 gcgccagcgc tgcaagatgg ccatgagtct cgtgcacgat cccgaagtcc tgatcctcga
     3181 cgaaccgacg gtgggtctgg acccgcccgg ccgcactcag ctcctcgagc tgatccgcgg
     3241 tctgcgcgac gaagggcggc gcatcctcat ctccacgcac atcctggccg acgccgaatt
     3301 cctgtgcgac gaaatgctgc tgctcgacgc cggcaccgtg gccttctcgg gaccggtggc
     3361 cgccatgatc aaggggcgcg aagagcgctt cgtggcctcg ggcgatcggc tcgatgcgac
     3421 gttcgtggcg cgactcgccc aatcgggaat ccccgccatg atcgacgacg ccggacggct
     3481 ggtcctgtcg gccaccgatg ccgaatcgat ccgccgcttc tggtcggtgg cttccgaatg
     3541 cggcgccgcc atccgtcgcc tcgatcgcga ggcaccgaca cttgaagagg cgctcctggc
     3601 ccggctcgac ttggtccgcg gcaatggagg tgcgcgatga gcgccatccc ctccgccggt
     3661 cacgtcatca ccacgatcgc ccagcgggaa ttccgcatcg cgctgcgccg ccggttcctc
     3721 cgcacgatcc tgatcctcgg cttcctgccg ccgatcggcg cggccgtcgt cctcatcatc
     3781 cgcctcctca tcaagcagat gcaggggaat gacgtcggct gggatcccct gcccttcctg
     3841 ctcaacttcc aggccatccc ggtcttcctg gccgccatcg gcctcggcac cccggccgta
     3901 tcgcgtgatc gcgcggaaga tgtgctctac ctgtatgccg tgcgccccgt gaaccccgcg
     3961 ttgtatgccg cggggaagat gctcgccgtc ttcctgccgg cgctcctcgt gatgctgctg
     4021 ccgatcgcca tggtcatcac cgtgcgccag ggaatattgg gaagcgatgc cgcgccgctc
     4081 gactcgctgg ccgatctggg gcgggccctg gtgctcacca tcggtctcgc cgcagccatg
     4141 gcgggcgcca ccgtaggccc ctcggccgct accaagcgcg gacgctgggc cctgctcctc
     4201 tcgctcaccc tcatgacgct ccccgacacc atcctgcgtg tcttcaacct ggatggcgac
     4261 tgggcgatcg gtccgctcaa cgtctcgcac aggctcgtcg agacgctctt cgtgagccac
     4321 gcctggatgg aatcgctcgg ccacatggcg atcctgctca cctgggggct ggccggtttc
     4381 ctcctgacgc gctggcgcgt ggcgcgggag atgatcccat gacgccggtc cgtctcgacg
     4441 ccgtcagcgt cttctacgga gaggtcgtcg gcctctcgca cgtgagcctc ggcctcgaac
     4501 cgggtctgac cggcgtggtg gggccgaacg gcagcggcaa gtccaccctg atgcgggtac
     4561 tcaccggtct cgtttcgccg cgcgaaggaa gcgtgaatgt cttcggaggc gatccgttcg
     4621 cgagcgccga gatccgctca cggattgcgt tcgtgcccgc caccgaaaac tttcatgacg
     4681 gcctgtcggg gcgcgacaac ctgcgattgc ttgcgacagc gatgggcctc accggcggcg
     4741 ccgtcggcgc gcgcgccgac aaggccctcg
//
LOCUS       DASVOD010000264         2617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_330384, whole genome shotgun sequence.
ACCESSION   DASVOD010000264 DASVOD010000000
VERSION     DASVOD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330384"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1947)
                     /locus_tag="VF720_05750"
     CDS             complement(<1..1947)
                     /locus_tag="VF720_05750"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX7878892.1"
                     /translation="MIRFGSAGIRRVRSAFVSFVLATFVAAAIPGHADEPGDGPAPGP
                     AAGEVSRPSGKGPARTTGPIERPYYFEPTPQGWPGETRDARDIAPYMAFHKARIAERI
                     NLMATTPTSNQSKWDARFYDIDVDLNPSLSRVTGTVIIKATVTNGPLTTLDLDFKANL
                     SLTEVRSAGIVVTSSRNVDVLTVNLDRSYANGELVTVQVKYFGTPTGGYFNFNTSGGQ
                     PMIWSLSEPFGARTWWPCKDYSDDKADSVDIQVRVPSNLITASNGTRIVNTSGGGFAT
                     TKWKERYPIATYLVFVASHPYTVATDWYVTALGDSVPLTFWMFPGSVATATPNNALVN
                     DMMTAFAPLFGEYPFANEKYGHAQFLFGGGMEHQTCTCLGVYIESITAHELAHQWWGD
                     KVTCATFNHIWLNEGFASYGEALWAEANGGAAARKAEMGLAKYFGAGTVYVPDLSDEN
                     RIFDSNLSYNKGSWVAHMLRGVVGDDDFFDGLQAYNTQYAYASATTENLRDAMEAQSG
                     MDLDRFFQQWVYGEYYPEYDYDWSVVPAGGGYDVHLTIDQTQSWQLFKMPITMRVSTA
                     GGDIDFTVNDSLATQSFVLHTALQPTDVALDPDEWILKSVVIPLGTPTFDRSILVVNG
                     VDWVTYGTEITNAYAAEAFWGDYNI"
     gene            complement(2028..2372)
                     /locus_tag="VF720_05755"
     CDS             complement(2028..2372)
                     /locus_tag="VF720_05755"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX7878893.1"
                     /translation="MVNHPRPIPAAIDLIADRDVVITWSDGSTTCHPAAALRARCPCA
                     TCGQETGGPNAHAPIKLGEPLPLMPAKARTDVTIRDVEAIGHYAIRFTFSDGHDTGIY
                     SYEMLREMNPPG"
     gene            complement(2389..>2617)
                     /locus_tag="VF720_05760"
     CDS             complement(2389..>2617)
                     /locus_tag="VF720_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878894.1"
                     /translation="QILLQPGMKASGNWVIAFAAAHPPLDQVPEFVAGLSGATAVFAP
                     VLLLLFAVINAWRVIRQVRARAGIQSNWRRV"
BASE COUNT          443 a          831 c          890 g          453 t
ORIGIN      
        1 aatgttgtaa tcgccccaga aggcctcggc cgcgtaggcg ttggtgatct cggtgccgta
       61 ggtgacccag tccacgccgt tcaccaccag gatgctccgg tcgaacgtgg gcgtgccgag
      121 cggaatgacc accgacttca ggatccactc gtcgggatcg agcgccacgt cggtgggctg
      181 gagcgcggtg tggagcacga acgactgcgt ggccagcgag tcgttcacgg tgaagtcgat
      241 gtcgccaccc gcggttgaaa cccgcatcgt gatcggcatc ttgaagagct gccacgactg
      301 cgtctggtcg atcgtgaggt gcacgtcgta gccgccgccc gccggaacca ccgaccagtc
      361 gtagtcgtac tcggggtagt actccccgta cacccactgc tggaagaaac ggtcgaggtc
      421 catgcccgac tgcgcctcca tggcatcgcg caggttctcg gtggtggccg aagcgtatgc
      481 gtactgcgtg ttgtacgcct ggaggccgtc gaagaagtcg tcgtcaccca ccacgccgcg
      541 cagcatgtgg gccacccacg aacccttgtt gtaggaaagg ttcgaatcga agatgcggtt
      601 ctcgtcggag aggtcgggca cgtagacggt gccggcaccg aaatacttgg ccaaccccat
      661 ctcggccttg cgcgcggccg ccccgccgtt ggcctcggcc cagagcgcct cgccgtaact
      721 cgcgaagccc tcgttcagcc agatgtggtt gaacgtggcg cacgtcacct tgtcgcccca
      781 ccactggtgc gccagttcgt gggccgtgat cgactcgatg tacaccccga ggcacgtgca
      841 ggtctggtgc tccatgccgc cgccgaagag gaactgggcg tgaccgtact tctcgttggc
      901 gaacggatac tcgccgaaga gaggcgcgaa ggccgtcatc atgtcgttca cgagggcgtt
      961 gttcggcgtg gcggtggcca ccgatcccgg gaacatccag aaggtgagcg gcaccgaatc
     1021 gcccagcgcc gtcacgtacc agtcagttgc caccgtatag gggtggctgg ccacgaacac
     1081 caggtacgtg gcgatgggat agcgctcctt ccacttcgtg gtggcgaagc cgccgcccga
     1141 cgtgttcacg atgcgcgtgc cattgctggc ggtgatcagg ttcgagggga cccgcacctg
     1201 gatgtcgacc gagtcggcct tgtcgtccga gtagtccttg cacggccacc aggtgcgcgc
     1261 gccgaacggc tcggagaggc tccagatcat cggctgcccg ccgctggtat tgaagttgaa
     1321 gtaaccgccg gtcggcgtgc cgaagtattt cacctgcacg gtgaccagct cgccgttggc
     1381 gtacgaacgg tcgaggttca cggtgagcac gtccacgttc cggctcgacg tgaccacgat
     1441 cccggcggag cgcacttccg tgagcgacag gttggccttg aagtcgaggt cgagcgtcgt
     1501 gagcggcccg ttggtcaccg tggccttgat gatgacggta ccggtcacgc gcgacagcga
     1561 cgggttcagg tcgacgtcga tgtcatagaa gcgggcgtcc cacttcgact ggttggacgt
     1621 cggcgtggtg gccatcaggt tgatccgctc ggcgatccgc gccttgtgga aggccatgta
     1681 gggtgcgatg tcgcgtgcgt cccgcgtctc gcccggccac ccctgcggcg tgggctcgaa
     1741 gtaatagggg cgctcgatcg gcccggtggt gcgggcgggg ccttttccgg agggccttga
     1801 tacctcccct gcagcgggcc cgggagcggg cccgtcgccg ggctcatcgg cgtgcccggg
     1861 gatggcggcg gccacgaaag tggccagaac gaacgaaacg aaggcggaac ggacgcggcg
     1921 gatgccggcg gagccgaaac ggatcatggt cggggtcctt tcggggtgcg tttcagtgga
     1981 cggatgatca taacatcccg ttccaactcg ccgacccgat ccccccacta tcccggcgga
     2041 ttcatctccc gcagcatctc gtaggaatag atccccgtgt catgcccgtc gctgaacgtg
     2101 aagcggatcg cgtagtgccc gatcgcctct acgtcgcgga tcgtgacatc ggtccgcgcc
     2161 ttcgccggca tgagcggcaa cggctccccc agcttgatcg gcgcatgggc gttcggcccg
     2221 cccgtctcct gcccgcaggt ggcgcagggg cacctggcgc ggagcgcagc cgcgggatgg
     2281 caggtcgtcg agccgtcgga ccaggtgatg acgacgtctc gatcggcgat gaggtcgatg
     2341 gcggcgggaa tggggcgggg atggttcacc atggaacggt cggccgcgtc agacccgccg
     2401 ccagttcgac tggattcccg ctcgcgcacg gacctggcgg atgacgcgcc aggcattgat
     2461 gacggcaaac aggagcagca atacgggcgc aaaaaccgcc gtggcccccg acaatccggc
     2521 cacgaactcc ggcacctgat ccagcggcgg atgggcggcc gcgaaggcaa tgacccagtt
     2581 acccgatgcc ttcatccccg gctgcagcag tatctga
//
LOCUS       DASVOD010000265         5878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_957494, whole genome shotgun sequence.
ACCESSION   DASVOD010000265 DASVOD010000000
VERSION     DASVOD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957494"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2002)
                     /locus_tag="VF720_05765"
     CDS             complement(<1..2002)
                     /locus_tag="VF720_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878895.1"
                     /translation="MTTPAVPARYIGLPGPGLRLRPYLKASDGGAADAHSPFVSRTSG
                     ALPYRAFLVAGKVDGQLLLPFVTILAAPDEYPGTKGSSSVPGAGNEQIEAVWQEAFRA
                     WRVVGEDGVAPLERRLLTDPDQLPRHAPVTFCTRIGDYFEPVCAECLGPLTVCRDEAR
                     LRRAGLPAWGDTLQRFLHCEACAGVGAAPVFYTAAARLPEGIAPGVRVRRRGELYRDL
                     APRITTGSSAPTSHHPCFTCAHRDECYPAGRRVDERVPAEDVLVPLFYYDTPFVPVAA
                     RPFTFAESAALIGGAAADDFASAPESVSMGDEGPLHQATLAGLLSEREQFLFAGDPTG
                     LLALEVFHLKLAAFGGLVRGVRALHAEAGRPHLALSPERIVGQLAPADGAPLPARWRL
                     TLEVADFVTTAPIVALERERLADEPRVASLPFPLPEAWLPEAMQRLPVTTLFMRMSPK
                     SVTKTETGTGTVVTVRAELTGNAFAEGDGGRHDLVRLSVESAAVEGGRLLLAGRRVET
                     LPSGFVFEGSSAPMPASVAAAVEGLRAASAEVNVHAVPSVPVDLVSLGILFIRFLLAN
                     DQQDVAALDRAVIERLAAAVAGDVGPEGRPPSDADRQYRARIGKALAAESIQGGPEQV
                     LYRAADRALAPAATPPSLWQDALVLALRMATNRPGFSLRGRLD"
     gene            2170..2736
                     /locus_tag="VF720_05770"
     CDS             2170..2736
                     /locus_tag="VF720_05770"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7878896.1"
                     /translation="MPTQPIGGVPPDATRPAAARPAPAAPADPDPVVGFLVFYSEMAE
                     APTGQPDPRLGRVYAMRESDVFFVGKAPLPEEVPVEGDRTEAPTAFHLFPASSDFAHI
                     SRRHAVLRMDRSGKIRVTDLSTNGLYFVRGKRHARRTSGSAPEVHELAAPETIVFGLD
                     ENSFKDPRWQDKADRFQVEIVPVGGAPR"
     gene            2733..3233
                     /locus_tag="VF720_05775"
     CDS             2733..3233
                     /locus_tag="VF720_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878897.1"
                     /translation="MKCEACYADNAAWTAVCLNCGKPVVPIELCENGHILPPGERECA
                     ACPKQTWPEFAPWSGPPLLRGVLLLEGAMIDLPGAAGRRWIELRNGADALGVAETAPG
                     RLGLVEGATNEGNLRLLVRPDGLRYCRRMAATGRLAWQEFPDGQTVSVGSYSIRWLAF
                     RAPARG"
     gene            complement(3261..5552)
                     /locus_tag="VF720_05780"
     CDS             complement(3261..5552)
                     /locus_tag="VF720_05780"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7878898.1"
                     /translation="MRRHYPDGSAALLLLLTLLVFLGHSPARANLPPLAPSFIEPDPG
                     EIVSAYDPHFNVFPFSDPDVGDAAAHSDWEVWDELLNVRVWADLAESVSVEHVHLRDG
                     ILEGPLASETHFAANRAYRVRTRFTDDSGAGNATGPWSPWVSFSTDSLDAIFPLKMQD
                     VLPDTVLFWRLENGDTVQLPPGPLPAALDVRTSLGRLVRLRGVPGGAYGIMNPPPRTG
                     DEPVKLILSAGSQELLLSFSTLSVNNQNGDRRSIYLPALVLAPGDSVFLWVSETGATW
                     YGTSAQSLPDFTQLARDVPVPWFLTRSGDVVERVASGFHLPVNLAFLPDPGQSPMDAF
                     FYVTELYGRVRVVTRNYTVLPLIDGLLNFDPLGGFPGSGEMGVNGITYDAALRALFVT
                     HVYDSLGAKYNRVIRLSLSPDRKSVTGYLRVFEGVPASASHQIGAVTIGPDGMLYVNI
                     GDGFVSTTAQNPLDMRGKILRMTKTGEIPPDNPFPGSRVYALGFRNPFGATWQPGTGR
                     LFISDNGDNANDRLVKVAAGSNYGWCCDMTRGALINFTPTVAPVALTFLPPGILSEGW
                     DDRLFLNWSGPAYRPGPSTRGKQIWTYALDDTGRIVASERFLTYAGTGYATCVGLATG
                     PDGLYFSDLYGEEGFNAAGITSGNIYRIRAGSVVSTPADPPGSGSRPQLAMSAAPNPF
                     RAEVTLSIQLTHPAEGDVLIVDVTGRRVASSGSRTWPAGLSTWRWDGRDARGRAVADG
                     VYFARITTTRGDSAVRKIFRSGR"
     gene            complement(5616..>5878)
                     /locus_tag="VF720_05785"
     CDS             complement(5616..>5878)
                     /locus_tag="VF720_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7878899.1"
                     /translation="TAISGAQINVAWGAALGIVENYQVERRRPNETFALRATMAGDVQ
                     LYADVGLLPGSTFEYRVRAMNATGPGGWSNVATATTPSPPTR"
BASE COUNT          944 a         2035 c         2067 g          832 t
ORIGIN      
        1 cgtcgaggcg gccccgcagg ctgaagcccg ggcggttggt ggccatccgg agcgcgagga
       61 ccagcgcgtc ctgccagagc gacggcggcg tggcggcggg agccagggcc cggtcggccg
      121 cgcggtagag cacctgttcg ggccctccct ggatcgattc tgccgccagc gccttgccga
      181 tgcgcgcacg gtactggcgg tcggcatccg acggcgggcg cccctcgggc ccgacatcac
      241 cggccaccgc ggcggccagc cgctcgatga ccgcgcgatc gagcgccgcc acgtcctgct
      301 ggtcgttggc caggaggaac cggatgaaga ggatgccgag gctcaccagg tcgaccggaa
      361 cggagggaac ggcgtgcacg ttcacctcgg ccgacgcggc tctcagtccc tccaccgccg
      421 ccgccaccga ggccggcatg ggcgcactcg atccctcgaa gacgaagccg ctcggcaatg
      481 tctcgacgcg acggcccgcc aggagcagcc ggcccccctc caccgcggcc gattcaaccg
      541 acaggcggac cagatcgtgc cggccgccat ccccttcggc gaaagcattc ccggtgagtt
      601 cggcccgcac ggtcacgacg gtgccggtgc ccgtttcggt cttcgtgacc gacttcggcg
      661 acatccgcat gaagagcgtg gtgaccggca accgctgcat cgcctccggg agccaggcct
      721 cgggaagcgg aaacggcagc gaggccacgc ggggctcgtc ggccaggcgt tcgcgctcga
      781 gcgccacgat gggcgcggtc gtcacgaagt cggccacctc gagcgtgagg cgccagcgcg
      841 cgggcaatgg cgccccgtcg gcgggcgcca gctgtcccac gattcgctcg ggcgaaagag
      901 ccaggtgggg ccgaccggcc tcggcatgca gcgcgcgcac cccacgcacg agcccaccga
      961 aggccgccag cttgagatga aagacctcga gggccagaag cccggtggga tcccccgcga
     1021 agaggaactg ctcgcgctcg gagagaagcc cggccagcgt ggcctggtgc agcggacctt
     1081 catcccccat ggatacggat tcgggggccg atgcgaaatc gtcggcggcc gccccaccga
     1141 tcagggccgc cgactcggcg aacgtgaagg ggcgcgccgc caccggcacg aacggtgtgt
     1201 cgtagtagaa gagcggcacc agcacgtctt cagcggggac gcgctcgtcc acgcgccggc
     1261 cggccggata gcactcgtcc cgatgcgcgc aggtgaagca ggggtgatgg ctggtcggcg
     1321 ccgaactgcc ggtcgtgatc cgcggcgcca ggtcgcgata aagctcgccg cgccggcgga
     1381 cccgcacgcc cggtgcaatc ccttcgggca ggcgcgcggc ggcggtatag aaaacaggcg
     1441 cagccccgac gccggcgcag gcttcgcagt gcaggaatcg ctggagcgtg tcgccccagg
     1501 ccggcagccc cgcccggcgg agccgggcct catcgcgaca gacggtaagc ggccccaggc
     1561 actcggcgca gaccggttcg aagtagtcac cgatccgcgt gcagaaggtg acgggtgcgt
     1621 gccgcgggag ctggtcggga tcggtcagca gccgccgttc cagcggcgct accccgtcct
     1681 cacccaccac gcgccacgcg cggaaggctt cctgccacac cgcttcgatc tgctcgttcc
     1741 ccgcgcccgg cacgctcgag ctccccttcg tccccggata ttcgtcgggg gccgccagga
     1801 tggtgacgaa cggcaggagg agctggccgt cgacttttcc cgccaccagg aaggcccggt
     1861 acggaagggc acccgacgtc cggctgacga atggggaatg cgcatcggcc gcgcccccgt
     1921 cactcgcctt gagatacggg cggagccgaa gacccgggcc cggcaatccg atgtaccgag
     1981 ccggaacggc aggcgtcgtc attggctcct ggggggatgg ggcgttaaaa gggggtcctg
     2041 tctggacttc gatcgaattg caccacttaa ggcccctagc gtcaatgccg atcgacggga
     2101 tcggatcgac acatccctcc ggcacgcgtg cgcacgtcgc cgcgccgggg taaactcccg
     2161 gcgatgtcct tgccgacgca accgatcggg ggtgttcccc ccgacgctac ccgacccgcc
     2221 gccgcgcgac cggcgcccgc ggcccccgcc gaccccgatc ccgtcgtcgg attcctggtt
     2281 ttctattccg aaatggccga ggccccgacc ggccagcccg atccccggct cggccgggta
     2341 tacgccatgc gggagtccga cgttttcttc gtcggaaagg ccccgctccc cgaggaagtg
     2401 cccgtggaag gggatcggac ggaggccccc accgccttcc acctcttccc cgcctcgagc
     2461 gactttgccc acatttccag aaggcacgcc gtcctccgga tggatcggtc cgggaagatc
     2521 cgggtgactg atctctccac caacggcctc tacttcgtcc gcggcaagcg ccacgcgcgc
     2581 cggacctcgg ggagcgcgcc ggaagtgcat gagctggcgg cgccggaaac gatcgtcttc
     2641 ggcctcgacg agaattcctt caaggacccg cgctggcagg acaaggccga ccgcttccag
     2701 gtggagatcg tcccggtggg aggcgccccc cggtgaagtg cgaagcctgt tacgccgaca
     2761 atgccgcctg gacggccgtc tgcctgaact gcggcaagcc ggtcgtgccg atcgagctgt
     2821 gcgagaacgg ccacatcctt ccccccggcg agcgggaatg cgccgcctgc ccgaagcaga
     2881 cctggccgga gttcgcgccc tggtccgggc cgccgctcct ccggggcgtt ctcctcctcg
     2941 agggggcgat gatcgatctg ccgggcgccg cggggcggcg ctggatcgaa ctccggaacg
     3001 gcgccgacgc gctcggcgtg gccgagacgg cgccggggcg gctcggactc gtggaaggcg
     3061 ccaccaatga gggaaacctg cgtctcctcg tccggccgga cggcctcagg tactgccggc
     3121 gcatggcggc caccggccga ctggcgtggc aggaatttcc ggacggccag acggtgtcgg
     3181 tcggttcgta ttccatccgc tggctggcgt tccgcgcgcc ggcgcgcggc tgacgagccg
     3241 gggttgctca ccccccggaa tcagcgtccc gaccggaaga tcttccgtac cgccgagtcg
     3301 ccgcgcgtcg tcgtgatccg cgcaaagtaa acgccgtcgg ccaccgcgcg tccgcgcgca
     3361 tcacgcccgt cccagcgcca ggtggagagg cccgccggcc aggtgcgtga ccctgatgac
     3421 gccacacggc gtccggtcac gtcgacgatc agcacgtctc cttccgcggg gtgggtgagc
     3481 tggatcgaca gcgtgacctc cgcgcggaac gggttcgggg ccgccgacat cgccagttgc
     3541 ggccgcgagc ccgaaccggg cggatccgcc ggcgtgctca ccacgctccc cgcccggatc
     3601 cggtagatgt tgccgctggt gatccccgcg gcgttgaatc cttcctcgcc gtagaggtcg
     3661 gagaagtaga gcccgtccgg ccccgttgcc agcccgacgc aggtggcgta cccggtgccg
     3721 gcgtaggtga ggaatcgctc ggacgccacg atcctgccgg tgtcatcgag cgcgtaggtc
     3781 cagatctgct tgccgcgcgt ggatgggccc gggcgataag ccggccccga ccagttgagg
     3841 aagagccggt catcccaccc ctccgaaaga atgcccggcg gcaggaacgt gagcgccacg
     3901 ggagccaccg tgggtgtgaa gttgatcagc gcgccgcgcg tcatgtcgca gcaccagccg
     3961 tagttcgagc cggcggccac cttcacgagg cggtcgttgg cgttgtcgcc gttgtcggaa
     4021 atgaagagtc gtccggtacc gggctgccac gtggcgccga acgggttgcg gaagccgagc
     4081 gcgtagacgc ggctgccggg aaaggggttg tcggggggaa tctccccagt tttcgtcatc
     4141 cggaggatct tgccgcgcat gtccagggga ttctgcgctg tcgtcgagac gaatccgtct
     4201 ccgatattca cgtagagcat cccgtcaggc ccgatcgtca cggccccgat ctggtggctg
     4261 gccgaagccg gcactccttc gaagacgcgc aggtagcccg tcacgctctt ccgatcgggc
     4321 gagagggaga ggcggatcac gcggttgtac ttggctccca gcgagtcgta cacgtgcgtc
     4381 acgaagaggg cgcgcagggc cgcgtcgtac gtgatgccgt tcacccccat ctcccccgag
     4441 ccgggaaagc cgccgagcgg gtcgaaattg aggagcccgt cgatcagcgg caggaccgtg
     4501 tagttgcggg tgacgacgcg cacccgcccg tacagttccg tcacgtagaa gaaggcgtcc
     4561 atcggcgact ggccgggatc gggaaggaag gccaggttca ccggcaggtg gaagccggag
     4621 gccactcgct ccaccacgtc gccggagcgc gtcaggaacc aggggaccgg cacgtcgcgc
     4681 gccagctggg tgaaatcggg caggctctgc gccgaagtgc cataccaggt ggcgccggtc
     4741 tcgctcaccc agaggaagac cgagtcgccg ggcgccagca ccagcgccgg caggtagatc
     4801 gaccggcgat cgccgttctg gttgttcacg ctgagcgttg agaacgagag caacagctcc
     4861 tgcgaaccgg cgctcaggat caacttgacc ggttcgtcac ccgtacgcgg tggcggattc
     4921 atgatcccgt acgcgccccc gggcacgccg cgcagccgca cgagccgacc gagcgaggtc
     4981 cgcacgtcga gcgcggccgg tagcggcccc ggcggcagct gtacggtgtc gccgttttcg
     5041 agccgccaga agaggaccgt gtcgggcagc acatcctgca tcttgagcgg gaagatggca
     5101 tcgagcgaat cggtcgagaa cgatacccag ggactccagg gcccggtggc gttgccggcg
     5161 cccgagtcgt cggtgaagcg cgtccggacg cgataggcgc gattggccgc gaagtgcgtc
     5221 tcggaggcca gcggcccctc caggatgccg tcgcgcaggt ggacatgctc cacgctgacc
     5281 gattcggcca ggtcggccca cacccgcacg ttcagcagtt cgtcccagac ttcccagtcg
     5341 gagtgcgcgg ccgcgtcgcc gacgtcggga tcgctgaagg ggaagacgtt gaagtgggga
     5401 tcgtaggccg acacgatctc gcccgggtcg ggctcgatga aggagggcgc gagcggcggc
     5461 aggttggccc gggcaggaga atgcccgaga aaaaccagca acgtcaggag gaggaggagg
     5521 gcggccgacc cgtccggata gtggcgtctc atgatgcgtg aaccattata gcggcggttc
     5581 cggcgggacg ctctctttta acgcgatgcg ccccgtcagc gggtgggcgg cgatggggtc
     5641 gtggcggtcg ccacgttgct ccaccccccg ggcccggtgg cgttcatggc ccggacgcgg
     5701 tattcgaacg tggatccggg caaaagaccc acatccgcat acagctggac atcgccggcc
     5761 atcgtggcac ggagcgcgaa agtctcgttc ggccggcgtc gctcgacctg gtaattctcg
     5821 acgatcccga gagccgcccc ccaggccacg ttgatctgcg cgccgctgat cgcggttg
//
LOCUS       DASVOD010000266        10286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_70497, whole genome shotgun sequence.
ACCESSION   DASVOD010000266 DASVOD010000000
VERSION     DASVOD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70497"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VF720_05790"
     CDS             <1..589
                     /locus_tag="VF720_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878900.1"
                     /translation="RVLAEVHRVLRPGGRFLLITWGGDAPDCPAMAAWDELLVAMGGP
                     IDDPEPPPTWYEEVDSAPRLRAMLAGVGFEAEWVTPGRGSFVFTPDHLAGMRLTVGGG
                     RRRYLALPANRRKAFEARGRRLLATLSPDDFHWRPEVITARASKPGRAGPADTPAGAA
                     DYRITCPNCGATMYDRGCKTRCPRCHFFTDCSDPW"
     gene            653..1441
                     /locus_tag="VF720_05795"
     CDS             653..1441
                     /locus_tag="VF720_05795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878901.1"
                     /translation="MAEFKEHAPGTFCWVDLAADDRAVAKKFYGELFGWTFEDVPAGP
                     DMTYTLAKLGGADVGGLGDLMPEQKLQGVPSHWMSYVAVTDADEALAKARSLGGTIVA
                     DAFDVMDVGRMGVVMDPDGAIFSVWQGRKTKGVMRRDEPGTLCWNELATRKSDVEGAF
                     YTNLFGWNTAPITGAGNMAYTMFQVGENGIGGMFEIKPEMGPMPPHWMPYMMVENVDA
                     SFAKAGTLGAKPIMPPTDIPNMGRFAIIHDPQGAAIAIYQPPGM"
     gene            complement(1483..2391)
                     /locus_tag="VF720_05800"
     CDS             complement(1483..2391)
                     /locus_tag="VF720_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX7878902.1"
                     /translation="MPTITYHRVNVFTHRPFGGNPLAVFPDAEGLPSDIMQSLAREIN
                     LSESTFVQRASAIGANVRVRIFTVAEELPMAGHPTLGTHFALARLGRYELTEPVTRVF
                     QEIGAGILPVDLMVRGGQVQKVVMTQAEPVFYEPIHEPGPLPEAFGIPESDFATPDLP
                     IQMVSTGLKQVMVPLRSLKALRRVVPRMDLVRQAAELFGSKMFYLFTRETIDPLSTTH
                     SRLVMPDLVGEDPATGSASGGLGAYLVRHKLVGHDPVVHIINEQGHEVGRPSRIEIEI
                     ENAYDGISSVKVGGPVVYLGQGTIDW"
     gene            complement(2399..2800)
                     /locus_tag="VF720_05805"
     CDS             complement(2399..2800)
                     /locus_tag="VF720_05805"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX7878903.1"
                     /translation="MTICPRCRFEYNPGAEWCPDCRVAMLRLVEWPAPTEPVDLGEDD
                     EWGDDPEPWSPSAPPDLTELRLLFEEPLPEMADMIREFLQNSGIACVVQANSLGHLYR
                     LMVPVGRARVYVHSDDFDEARELVQQFFGTS"
     gene            2969..4282
                     /locus_tag="VF720_05810"
     CDS             2969..4282
                     /locus_tag="VF720_05810"
                     /inference="COORDINATES: protein motif:HMM:NF025068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878904.1"
                     /translation="MKPIPYLLLPLALLAGVAARPTPARADRTVRVAVAADEEYRRHG
                     DWKELAEVGVAGAEKGFYPFGLRFEVVEFLAWDSDASDQDVAALQNELSAEVRSTRAE
                     LILGFAGAVTPRSNRLFVRLGHSDTPGRYLLVSERAGRDLDLVLRHELGHAFGLPHVS
                     HTRSVMNEGVEGDRKEFDSLSGAILRNNARLDFLASDPLAGCNLGNLWALYDQVARND
                     DDVADLIAIIGDSYRRRGDAASADRAYRSAESMESGLVSARLGLAMLALAEGRPSESV
                     TLLEQVRADAGTIAGLETSLGLAYSQLGLTSSATRAYETAIRQDPNDAAALNNLGLLR
                     MNDDRNDEAERFLKQALAVRPRFSEAWNNYGTLLQKLGRYDEAIEAFQSSLSIGETAI
                     AHRNMAGLLLKQHRDAEARRHIQATLRLDPDQSDADSLRRLADRR"
     gene            4342..5256
                     /locus_tag="VF720_05815"
     CDS             4342..5256
                     /locus_tag="VF720_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7878905.1"
                     /translation="MKSYDVVGLGYACLDILGTVPHLPELDTKLGLGALVIQGGGPVA
                     TALVTLARLGSRTLFAGRLGDDDFGRAIISGLKDEHVGLEVHVSPGTTSPFAFVMVTP
                     DGKRTVLWTRGTAPLMAPDDVQAEVVRAGRLLLLDDIEIPAATRAASLAREAGVPVVL
                     GVGTPRAGIEGLIRLADDIVMAEKFPFRLTGERDHPRALEAILAMGPARATVTLGERG
                     SLHLDRSGAWLEQAAIPAATVDTTGAGDVYCGAYVFGRLEGWPMERTLRFASCVAARQ
                     TEGLGGRTAIPTRAEALLLADAAKTITQ"
     gene            5277..7160
                     /locus_tag="VF720_05820"
     CDS             5277..7160
                     /locus_tag="VF720_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7878906.1"
                     /translation="MIHTRSTGRDAPGPADLIRAGVGGGLVLGLSLARLTWQMNPHLS
                     GHAGDIGLLFLYFAIGYGLAGAAVGAAGALVLGLIRTVAGRTSMITTLLGTAGLAGLL
                     LGPMVYYALLPDVGWQHGFHSGLLFGASRPRLLLSLLGLVAALSVTGWLLRPVVQSLA
                     DSLRVSSRTLLVRLVILAGVLIVALGAAFPPRPAEPVVAAPTSLAAITPPDSSRAPVV
                     LLCIDGADLDDMILPMTAKGELPVFAHLMRAGTWGRLATIEPTLSAVVWTTIITGKTP
                     DQHGIRHFIVFRLPGIRKAIHQFPRHTGLNFRIFPMLEKLPGMPSLQAPYTSNMRTAE
                     ALWNIAGRFMPVGAYRWLVTWPVEPVNGFNVAGGVGWLQIMGGFENEAHDLLSKGFHY
                     PHEVYAGLPLPTPIPDVTKSMLEPYVGAGHDVPPTDWKVKAIAAALRDPTGHYLPLLQ
                     HKFGARFTAASFYPVDEFCHYFAVNKDKGGIYSNAVAERYRYTDARLGEYLAALGDSV
                     NVVIVSDHGYDFVQNHHTNAPAGVFFGIGPDFAAGKRLDTLNVYDVAPLCLELLGLPP
                     GADMPATAPRRYEAALLHGRGTNAPAAVATWETGERRADDPISSPIDAQIKEQLRSLG
                     YIN"
     gene            complement(7221..8045)
                     /locus_tag="VF720_05825"
     CDS             complement(7221..8045)
                     /locus_tag="VF720_05825"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7878907.1"
                     /translation="MAPLKSCPSCRALVDSTETTCPYCGAQTDRRSVREVHLGASDTP
                     GLVTGWFIGACALFFILEMVATLGAAGTRGLWQALVGVPGAILFEMGARATQRILAGE
                     WWRLIVPMFLHGNFMHILFNTMALIQAGPLAEQAYGRSRFIVLFLVTGALGFLLGTYT
                     SPMSISIGASGSLFGLIGAAGLYGHRRGDAFGRMIRGTMIQWALYSFLFGLLIRADNA
                     AHLGGLVSGIALAFVMDDRHGRAADTFWKIAAGVCVAVTLVAFALAIASYLGVRAG"
     gene            complement(8059..8310)
                     /locus_tag="VF720_05830"
     CDS             complement(8059..8310)
                     /locus_tag="VF720_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878908.1"
                     /translation="MDWTPEAAEKFKLLLGEIPVLMRPLAEQMGRNEVGKAAAARGVT
                     QVDLATMVIGLVKATPPHLKEKMQEAMTKHGINPSDYTG"
     gene            complement(8356..9981)
                     /locus_tag="VF720_05835"
     CDS             complement(8356..9981)
                     /locus_tag="VF720_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878909.1"
                     /translation="MPRGVVAPPAVVKLLSRLPLPGLEPRLSWDWDPTAFEPLAHPVP
                     DDFAWRHRELAKSNLPDLVRARLNLELAFLGAAHRDGPLELRRIANPDSMSSEARALR
                     SRDAYLRLLETAPDNGEFRTDLGDLYRFLGQPDSAIVQYRRALMSARPPARLFSRLAN
                     AELEQLIQNPSAGTQSEFRNVLARGMAHFAGAAPTDPRRAARFHLERAKFRVDRAVLE
                     VQADRAMRKEAWRQAGPDSTFALMARVVAPETRRAFQAAAESDTNLAEAHGWLGTVVT
                     GQIYLPITAEGWLTRSTMTSRDTLAAALWKLAVRRRAQRSGDAAYAAANLNRCEALEP
                     TRFPRARAEQARLAVILDDLDGSAGLWRALMVRDPENIETYAAELFTTHSLEGAAGLE
                     TDLPSAGRVERGISEALREKTGAYGANNSEAVTLLGILRGELGRPEEAWRDLSRSAAF
                     DTTDWRARLGLAVLSLHEMDAPAAEPHLAYAGRHFGQLDEPARGIYCGAVGLLYAARR
                     DTVNARRWLDEAVRFNPAEPVLREAARSLDAGP"
     gene            complement(10070..>10286)
                     /locus_tag="VF720_05840"
     CDS             complement(10070..>10286)
                     /locus_tag="VF720_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878910.1"
                     /translation="RFALKEGEKLPPGITKHFIPNRALKVNLPLSVLKGKKTLATRGR
                     ELSAWIEKKSREKKIRAYPEPTVIFDE"
BASE COUNT         1596 a         3619 c         3419 g         1652 t
ORIGIN      
        1 acgcgtgctg gccgaggtgc accgcgtgct gcgccccggc ggtcggttcc tcctgatcac
       61 ctggggaggc gacgctcccg actgtccggc catggctgcc tgggatgagc tgctcgtcgc
      121 gatggggggc ccgatcgacg accccgagcc gccgccgacc tggtacgaag aggtcgattc
      181 cgcgccgcgg ctccgcgcca tgctcgctgg ggttggattc gaagccgagt gggtcacgcc
      241 gggacgcggg tcgttcgtct ttacacccga tcacctggcc gggatgcgcc tcacggtcgg
      301 gggaggccgg cgacgctacc tggccctgcc ggccaaccgt cgtaaggcgt tcgaggccag
      361 gggacgccgg ctcctcgcca ctctttcgcc cgatgacttc cactggcgcc ccgaagtcat
      421 cacggcgcgg gcgtccaaac cgggcagggc cggtccggcc gatactcccg ccggcgcggc
      481 ggactaccgg atcacctgtc ccaactgcgg ggccaccatg tacgaccgcg gttgtaaaac
      541 ccgttgcccc cgctgccatt tcttcaccga ttgctcggat ccctggtagt cgccccggga
      601 catcggacgt aagcttttcg tccgtccatc acccgaaacg aaggagcgca ccatggcaga
      661 gttcaaggag catgcccccg gcacgttctg ctgggtggac ctggccgctg atgaccgggc
      721 cgtcgcgaag aagttctacg gcgagctgtt cggctggaca ttcgaggacg tgcccgccgg
      781 accggacatg acctacacgc tcgcgaagct aggtggggcc gacgtcggcg gcctcggcga
      841 cctgatgccg gaacagaaac tccagggcgt cccctcacac tggatgtcct acgtggccgt
      901 gaccgacgcg gacgaggcgc tcgcgaaggc caggtcgctg ggcggcacga tcgtggccga
      961 tgcgttcgac gtcatggacg tcggtcgcat gggagtcgtc atggatcccg atggggccat
     1021 cttctcggtc tggcagggaa ggaagaccaa gggggtcatg cggcgcgacg agcccggcac
     1081 cctctgctgg aacgaactgg ccacgcggaa gagcgacgtg gaaggcgcgt tctacacgaa
     1141 cctcttcggc tggaacaccg cgccgatcac cggcgccggc aacatggcct acacgatgtt
     1201 ccaggtcggc gaaaacggca tcggcgggat gttcgagatc aaaccggaaa tgggccccat
     1261 gccgccgcac tggatgccct acatgatggt cgagaacgtc gacgcctcgt tcgcgaaggc
     1321 cgggacgctc ggcgccaagc cgatcatgcc gccgaccgac atcccgaaca tgggtcggtt
     1381 cgccatcatc cacgatccgc agggtgcagc catcgccatc taccagccgc cgggcatgta
     1441 gcctttggca gggcgcgggc ggtccgggag ccgggccgcc cgttaccagt cgatcgttcc
     1501 ctgccccagg tacaccaccg gcccgcccac cttcaccgac gagatgccgt cgtaggcgtt
     1561 ctcgatctcg atttcgatcc ggctcggccg ccccacctcg tgtccctgct cgttgatgat
     1621 gtggaccacc ggatcgtgcc cgaccagctt gtgccgcacg aggtaggccc ccagcccccc
     1681 ggaggcgctg ccggtggccg gatcctcgcc gaccaggtcg ggcatcacga ggcggctgtg
     1741 cgtggtggac aggggatcga tcgtctcgcg cgtgaagaga tagaacatct tgctgccgaa
     1801 cagctcggcg gcctggcgca ccaggtccat ccgcggcacc acgcggcgca gcgccttgag
     1861 ggagcggagc ggcaccatta cctgcttgag cccggtcgac accatctgga tcggcaggtc
     1921 cggcgtggcg aaatccgatt ccggaatgcc gaaggcctcg gggagcgggc cgggttcgtg
     1981 gatcggttcg tagaagaccg gctcggcctg tgtcatcacc actttctgca cctgcccgcc
     2041 cctcaccatg agatccaccg ggaggatgcc ggcgccgatc tcctggaaga cgcgggtcac
     2101 cggttcggtg agctcgtatc gccccagccg ggccagcgcg aagtgggtgc cgagcgtggg
     2161 atgtcccgcc atcggcaatt cctcggccac cgtgaagatg cgcacgcgaa cgttggcgcc
     2221 gatggcgctg gcccgctgca cgaaggtcga ttccgagaga tttatttccc gggccaggct
     2281 ctgcatgatg tcggagggga ggccttcggc atccgggaag acggccagcg gattgccgcc
     2341 gaacggccgg tgggtgaaga cattgacccg gtgataggtg atggtcggca cggggtgatc
     2401 agctggtccc gaagaactgc tggaccagct cgcgggcttc gtcgaagtcg tccgagtgca
     2461 cgtagacgcg ggcccggccg accggcacca tcaaccggta gagatgaccc agtgagttgg
     2521 cctgcacgac gcaggcgatc ccggagttct ggaggaattc gcggatcatg tcggccatct
     2581 cgggaagcgg ctcctcgaaa agcagtctca actccgtcag gtcgggagga gccgaaggcg
     2641 accagggttc ggggtcatcg ccccattcgt cgtcctcgcc caggtcgacc ggctccgtcg
     2701 gcgcaggcca ctccacgagc cggagcatgg ccacgcgaca atccgggcac cactcggcgc
     2761 ccgggttgta ttcgaatcgg cagcgtgggc agatggtcat ctggttgcac tcccttgtcc
     2821 gacaacacta taacaagtcc gggagcgctt tcgcgatgac ggggcgcgcg ggaaccgccg
     2881 ggctggacgc gagtgctttg cctgccgata accggaccat ccgggttccg cctcgcgccg
     2941 aggccccggc tccgtcccgg ggagacacgt gaaaccgatc ccgtaccttc tccttcccct
     3001 cgcgctgctc gccggggtgg ccgcccgacc gacccccgcc cgggccgacc ggaccgtccg
     3061 cgtggccgtg gccgccgacg aggaataccg ccgccacggc gactggaagg agctggcgga
     3121 ggtcggggtg gccggggccg aaaagggctt ctaccccttc ggcctccggt tcgaggttgt
     3181 ggaattcctt gcctgggatt ccgatgcctc cgaccaggac gtggcggccc tgcagaacga
     3241 actctccgcc gaggtgaggt cgacccgggc cgaactgatc ctcggtttcg ccggggcggt
     3301 cacgccgcgg agcaatcggc tcttcgtgcg ccttggccat tcggatacgc cggggcgcta
     3361 tctcctcgtg tccgagcgcg ccggccgcga tctcgacctc gtcctgcggc acgagctcgg
     3421 ccatgccttc ggcctgcccc atgtctcgca tacccgctcc gtcatgaacg aaggggtgga
     3481 gggagatcga aaggagttcg attcgctgag cggcgccatc cttcgcaaca acgcccggct
     3541 cgacttcctg gcgagtgacc cgttggccgg ctgcaacctc gggaacctct gggcgctgta
     3601 cgaccaggtg gcgcgtaacg acgacgacgt ggcggacctc atcgccatca tcggcgacag
     3661 ctatcgccgc cggggcgatg ccgcctcggc ggaccgcgcc taccgatcgg ctgagtcgat
     3721 ggagtcgggc ctcgtctccg cccggctcgg cctggccatg ctggcccttg ccgaggggcg
     3781 gccatccgag tcggtgacgc tgctcgagca ggtgcgtgcc gacgccggca ccattgccgg
     3841 tctcgagaca agcctcggcc tcgcctactc gcagctcggc ctcacttcca gcgccacccg
     3901 ggcctacgaa actgccattc gccaggatcc caatgacgcg gcggccctga acaacctggg
     3961 cctcctccgg atgaatgacg accggaatga cgaagccgag cgcttcttga agcaggcgct
     4021 tgccgtgcgg ccccgattca gcgaagcgtg gaacaactac ggcacgctgc tccagaagct
     4081 ggggcggtac gatgaggcca tcgaggcctt ccaatcctcc ctgtcgatcg gggagacggc
     4141 gatcgcccac cggaacatgg ccgggcttct cctcaagcaa caccgcgacg ccgaggcccg
     4201 tcgccacatc caggccaccc tgcgtctcga ccccgaccag tccgacgccg acagcctccg
     4261 ccggctggcc gatcggcgat aggcctgata gcctgaaggg gttcccggtt cgacatccga
     4321 acccccacct ggccagggac catgaaaagc tacgacgtcg tcggcctggg atatgcctgc
     4381 ctcgacatcc tcggaacggt gccccacctg cccgaactcg ataccaagct cggtctcggc
     4441 gcgctggtca tccagggtgg cggtccggtc gccacggcgc tggtcaccct ggcccggctc
     4501 gggtcgcgca ccctcttcgc cgggcgcctc ggcgacgacg atttcggccg cgccatcatt
     4561 tcgggcctca aggacgagca tgtcggtctc gaggtccatg tctcaccggg tacgaccagt
     4621 cccttcgcct tcgtgatggt gacaccggac ggcaagcgga cggtgctctg gacgcggggc
     4681 accgcgcccc tcatggcgcc ggacgacgtc caggccgagg tggtgcgggc cggacgcctc
     4741 ctcctcctcg acgacatcga gatcccggcg gccacccggg cggcgagcct cgcccgggaa
     4801 gccggggtcc cggtcgtgct cggtgtgggc acgccgcgcg ccggcatcga gggattgatc
     4861 cggctggccg atgacatcgt catggccgag aaatttccct tccgcctgac cggcgagcgc
     4921 gatcatcccc gggccctcga agccatcctg gcgatgggac ctgcccgcgc caccgtcacg
     4981 ctcggcgaga ggggatcgct ccacctcgac cgcagcggcg cctggctcga gcaggcggcc
     5041 attccggcgg ccacggtcga cacgaccggc gccggcgatg tctactgcgg cgcgtacgtt
     5101 ttcgggcgcc tcgagggttg gccgatggaa cggacgctcc gcttcgcgtc atgcgtggcc
     5161 gcccggcaga ccgaagggct cggcggccgc acggccatcc cgacacgcgc cgaggccttg
     5221 ctcctggccg acgccgcgaa gaccatcacc cagtaatcga ttcgatcggg aggccgttga
     5281 tccacacgcg aagcaccgga cgggatgcgc ccggtcccgc cgacctcatc cgggcgggag
     5341 tgggaggggg cctcgtcctc ggcctctccc tggcccggct tacctggcag atgaacccgc
     5401 atctctccgg ccatgccggg gacatcgggc ttctcttcct ctactttgcc atcggctacg
     5461 ggttggccgg tgcggccgtg ggcgcggcag gcgccctcgt gctcgggctt atccgcacgg
     5521 ttgccggccg cacatccatg atcaccacgc tccttggaac cgccggactg gccgggctcc
     5581 tcctggggcc gatggtgtac tacgcgctgc tccccgatgt cggctggcag catggcttcc
     5641 attccgggct tctcttcggc gcttcccggc cgcggctcct tctctccctt ctcgggctgg
     5701 tggcggcgct gtcggtgacc ggttggctgc tccgaccggt ggtccaatcg ctggccgact
     5761 cgttgcgtgt ctcgagccgc accctccttg tgcgtctcgt catcctggcc ggtgtcctga
     5821 tcgtggcgct cggtgcggcc ttcccaccgc ggccggccga gccggtcgtt gccgcaccga
     5881 cctcgctggc ggctatcacc ccacccgatt cgagccgggc tccggtggtc ctcctctgca
     5941 tcgacggcgc cgacctcgac gacatgatcc tgccgatgac ggccaagggc gagctcccgg
     6001 tcttcgcgca cctcatgcgg gccggaacat ggggacgcct cgccaccatc gagcccacgc
     6061 tctccgccgt cgtctggacc accatcatca ccggcaagac gcccgaccag cacggcatcc
     6121 gccacttcat cgtcttccgc ctgccgggaa tccggaaggc catccaccag tttccccggc
     6181 acaccggcct caacttccgc atcttcccga tgctcgagaa gctcccgggg atgccctcgc
     6241 tgcaggcgcc ctacaccagc aacatgcgca cggccgaggc actctggaac atcgcgggtc
     6301 ggttcatgcc ggtgggtgcg taccggtggc tcgtcacctg gcccgtcgag ccggtcaacg
     6361 gcttcaatgt ggccggcggt gtggggtggc tgcagatcat gggaggattc gagaacgagg
     6421 cgcacgacct cctctcgaag gggtttcatt atccgcacga ggtgtacgcc ggacttccgc
     6481 ttcctacgcc catacccgac gtgacgaagt cgatgctgga gccctacgtc ggcgccggcc
     6541 acgacgtgcc gccgaccgac tggaaggtga aggccatcgc cgcggcgctg cgcgacccga
     6601 ccggtcacta cctgccgttg ctccagcaca agttcggcgc gcgcttcacg gccgccagct
     6661 tctatccggt ggacgagttc tgccactact ttgccgtgaa caaggacaag ggtgggatct
     6721 actcgaacgc cgtggccgaa cggtatcgct acaccgatgc ccggctcggc gaatatcttg
     6781 ccgcgctggg tgattccgtc aacgtcgtca tcgtgtccga tcacggctac gacttcgtgc
     6841 agaaccacca cacgaatgca ccggccggcg ttttcttcgg catcggcccc gatttcgcag
     6901 cggggaagcg gctcgacacg ctcaacgtgt atgacgtggc gccgctctgc cttgagctcc
     6961 tcgggttgcc gccgggagcc gacatgccgg ccaccgcgcc gcgccgctac gaggcggcct
     7021 tgctgcacgg gcgcggcaca aacgcgccgg cggccgtggc cacctgggaa acgggggagc
     7081 gccgcgccga cgatccgatc tcgagcccga tcgatgccca gatcaaggaa cagctgcgga
     7141 gcctgggcta catcaactga ccggcggcaa atcggcggaa cggcggccgc tcggcggaac
     7201 gacggccggt tcaacaccgc tcagccggcg cgcaccccca ggtacgaggc gatggccagc
     7261 gcgaaggcca ccagcgtcac cgccacgcag acaccggccg cgatcttcca gaacgtatcg
     7321 gcggcgcggc cgtggcggtc atccatcacg aacgccagcg cgattccgct cacgagtccg
     7381 cccaggtgcg ccgcgttgtc ggctcggatg agcaggccga agaggaacga gtagagggcc
     7441 cactggatca tggtgccgcg gatcatccgg ccgaaggcat cgcctcggcg gtggccgtag
     7501 aggccggccg cgccgatcag gccgaagagg gagccggagg cgccgatgga aatggacatc
     7561 ggcgaggtgt acgtgccgag caggaacccc agcgcccccg tcacgaggaa gaggacgatg
     7621 aaccggctcc gcccgtaggc ctgctccgcc agtggcccgg cctggatcag cgccatggtg
     7681 ttgaagagga tgtgcatgaa attgccgtgc aggaacatcg gcacgatcag ccgccaccac
     7741 tcgccggcca ggatgcgttg cgtggcccgg gcgcccatct cgaacaggat ggcccccggc
     7801 actcccacca gcgcctgcca caaccccctt gtgcccgcgg cgccgagcgt ggccaccatc
     7861 tcgaggatga agaagagggc gcacgccccg atgaaccagc cggtcacgag ccccggcgta
     7921 tcggacgcac ccaggtgaac ctcgcgcacg ctccggcgat cggtctgggc gccacagtac
     7981 gggcaggtcg tctcggtcga gtcgaccagc gcccggcacg aggggcacga cttcagcggt
     8041 gccacgtggc tcccggaatc aaccggtgta gtccgaggga ttgatgccgt gcttggtcat
     8101 ggcttcctgc atcttctcct tcaggtgggg cggcgtggcc ttcacgaggc cgatgaccat
     8161 cgtggccagg tccacctgcg tgacgccgcg cgccgcggcc gcctttccca cctcgttccg
     8221 ccccatctgc tcggccagcg gccgcatcag cacggggatt tcaccaagga ggagcttgaa
     8281 tttctcggcg gcttccggtg tccagtccat gtccttctcc ttggttgatt cgggccccct
     8341 gtctcgggca cctgcctacg ggcccgcgtc gaggctgcgg gccgcctccc ggagcaccgg
     8401 ttcggcggga ttgaatcgga ccgcttcgtc gagccagcgg cgcgcgttga cggtatcccg
     8461 gcgggccgcg tacaggaggc ccaccgcgcc acagtaaatc ccccgggccg gctcgtcgag
     8521 ttgtccgaaa tgtcttccag cgtaggcgag gtggggctcg gccgcgggtg catccatctc
     8581 gtggagcgac agcacggcca ggcccagccg cgcgcgccag tcggtcgtgt cgaaggccgc
     8641 cgaacgggag aggtcacgcc aggcctcttc cggacggccg agctccccgc gcaggatccc
     8701 gagaagtgtc accgcctccg aattgtttgc cccgtaggcc ccggttttct cgcgaagcgc
     8761 ctcgctgatc ccccgctcca cacgccccgc cgatggcagg tcggtctcga gcccggccgc
     8821 tccttccagc gagtgggtgg tgaacaactc cgcggcatac gtctcgatgt tctccgggtc
     8881 gcgcaccatc agcgcgcgcc acagtccggc cgacccgtcg aggtcgtcga ggatcaccgc
     8941 cagccgggcc tgttccgccc gcgcccgggg aaaccgcgtc ggctcgagcg cctcgcagcg
     9001 attgaggttg gcggccgcgt acgccgcgtc tccggagcgc tgggcccgac gccggacggc
     9061 cagcttccag agcgccgccg ccaatgtgtc gcgcgatgtc atggtcgagc gcgtgagcca
     9121 cccctctgcg gtgatcggca ggtagatctg cccggtcacc acggtgccga gccagccatg
     9181 cgcctcggcc aggttggtat cgctctcggc ggccgcctgg aacgcccggc gcgtttcggg
     9241 cgccaccacg cgcgccatca gcgcgaacgt cgaatcggga ccggcctgcc gccacgcctc
     9301 ctttcgcatc gcgcggtcgg cctgcacctc gagtacggcg cggtccaccc ggaactttgc
     9361 ccgttccagg tgaaagcgcg ccgcccggcg cggatcggtc ggcgccgccc ccgcgaagtg
     9421 ggccatcccc ctggccagca cgttgcgaaa ctcggattgc gtgccggccg acgggttctg
     9481 gatcagctgc tcgagttcgg cattggccag tcgtgaaaag aggcgtgccg gcggccgggc
     9541 cgacatgagc gcccgccggt actgcacgat cgccgaatcg ggctggccca ggaaacggta
     9601 gagatctccc aggtcggtcc ggaattcgcc gttgtcgggc gcggtttcaa ggagacggag
     9661 ataggcatcg cgtgaccgga gagcccgagc ttcgctcgac atcgaatcgg gattggcaat
     9721 tcgcctcagt tccaggggcc cgtcgcggtg cgcggcgccg aggaaagcca gctcgaggtt
     9781 gaggcgggcc cgtaccaggt ccggcaggtt ggacttcgcg agctcacgat gccgccacgc
     9841 gaaatcgtcc ggcacggggt gggccagcgg ttcgaacgcc gtgggatccc agtcccagga
     9901 gaggcgcggt tcgagcccgg ggagggggag ccgcgagagg agtttcacga cggccggcgg
     9961 agccacgacg ccccggggca tcgcgaggct ccgtggtggt tctgcgcccc atcccggcga
    10021 tcctgccagg acggccgcga gcatcgccac cccccggacc atccgggaac tactcatcga
    10081 agatgaccgt cggctcggga taggcgcgga tcttcttttc gcgactcttc ttctcgatcc
    10141 aggccgacag ctcgcggccg cgggtggcca gcgtcttctt ccccttcagg accgacagcg
    10201 gcaggtttac cttgagcgcc cggttcggga tgaagtgctt ggtgattccc ggcggcagtt
    10261 tctcaccttc cttgagcgcg aaacgg
//
LOCUS       DASVOD010000267        16501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_695566, whole genome shotgun sequence.
ACCESSION   DASVOD010000267 DASVOD010000000
VERSION     DASVOD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 16501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16501
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695566"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VF720_05845"
     CDS             complement(<1..1510)
                     /locus_tag="VF720_05845"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEX7878911.1"
                     /translation="MTKHSALDSWVKETAALCQPDQVVWCDGSPEEYQRMLHLMVQTG
                     TAQWLDPARRPNSIFVRSDPADVARVEEFTFICSKSKDDAGPTNNWKDPAEMKATLTP
                     LFNGCMAGRTMYVIPYSMGPVGSPIAKIGVEISDSPYVVANMHIMARVGTKVLDVLGT
                     NGEFVRGMHSIGSPITGNAPDSPWPCNAKTKYISHFPESREIWSYGSGYGGNALLGKK
                     CHALRIASVQARDEGWMAEHMLILGITDPKGQKIYVAAAFPSACGKTNLAMMNPTLPG
                     WKIETVGDDIAWMKYGQDGRLYAINPEAGFFGVAPGTSMKSNPNAMITARENSIFTNC
                     ALTPDNDVWWEKMTDAPPATLVDWLRRPWTPRAGRPAAHPNARFTTPARQCPVISSEW
                     ENPAGVPISAILFGGRRATVVPLVNEARSWQHGTFLGSIMGSEMTAAAAGTVGQLRHD
                     PFAMLPFCGYHMGDYFKHWLSIGAKGDPNKLPRIYQVNWFRKNNDGDFLWPGY"
     gene            complement(1589..2605)
                     /locus_tag="VF720_05850"
     CDS             complement(1589..2605)
                     /locus_tag="VF720_05850"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX7878912.1"
                     /translation="MRNRVTLLMLAALLVLPSGARAMCSCFPCDECQIVNSGQATFIM
                     FDREGGTVRLIPNILITGPAASFALIVPTPTVPELAPVDKALWSQLSTLTQPPVTSFG
                     GSSGGPGCSQVDESPSPADNSGTEIIATRSIGSFIATTIHSSDPRSLVSWLFSNGFEV
                     TEAEAQALARYVSIGWYFTAMKLDPESPESQVPDGGWFVNVNPVEFRYAADRVDVPLD
                     LLGINRSDFFPVRFYVVDDHRRTLEGYQTNYANQLSEKELDAMGQSQPLAAGYLAPGR
                     FLTRLERTYGYQDVLEGSLPLVVADSDEEVYWPDRRFGPTFPLESLVFLLLPAGLLRR
                     RRER"
     gene            complement(2623..3249)
                     /locus_tag="VF720_05855"
     CDS             complement(2623..3249)
                     /locus_tag="VF720_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878913.1"
                     /translation="MPHRRPIRVLPLVLAPLLVPVNFPAFLSPPARAGEARLSLSGGI
                     HSPLGGDERERFGIAPQFGLGLATEFADGASMVGLDVGYVHSGGGERYISTFEFPESH
                     YTLVPVTVSVTTRLAPESRESWPRVFVGFFVTVMPTWFTDRNEETFSTPTVGAGLELR
                     PEFHLASDWRAFGRTRLLLVEPVDYQAALDDINYSALSLELGVSTGLH"
     gene            3395..6097
                     /locus_tag="VF720_05860"
     CDS             3395..6097
                     /locus_tag="VF720_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7878914.1"
                     /translation="MTPERLGGFRIDEEIGAGGMGVVYRAHDEKLGRAVAIKVLPERV
                     AADPERLARFDREARLLASLNHPNVAILHGLDQDGDTRFLVMELVGGESLEKRLRNGA
                     MPLAKALPIALQVASALEAAHAQGIVHRDLKPGNVMLTPEGRVKLLDFGLAKMLSGDS
                     GSFRDSGTRATTVSGYLMGTPGYMSPEQASGDQVDRRADVWAFGCLLFEMVSGKRAFG
                     GESLTQMLTAILSVDPDWALLPADTPAGIRRLLRRCLAKDPNRRLHEMADARIEIEET
                     LAPPSGATGEFAVVGRAESIADSPGHGRASPSPVATRAAGVPPWLVIAGLLLVIVLAG
                     MAMPLLMKGSGRQAGREPGPRRYSIAMPDSMNLAGDYRPTVRISPDGRWLAASVYTAD
                     WQRRTLIEDLVTGAVRIVARPDVLTLGFSPDSREAYMETRDARWVEELSTGTLRPLRH
                     DDIEATPLGIIWIDDGFIVQPSFGRPLLRLKADGSSLRSLAPIDSSTGEICHLDPRRI
                     PGTDWILFTVFSGGEWTSARIEAMNLTTGERRRVLDGAIGGRATADGWLLHLRNTDLL
                     AMRFDPKRMTVSGTARTVAHDVQFFPTTGLASYDVSDDGTLVYVTGGVADIRETFTWL
                     DEAGNETPTAIPPGGYADASLSPDGTRVAMTLEGTRYQIGIAKIAGGPVREFTFNSDD
                     NYARWSPDGRMLAYTGDSPRRAVMLRPVDGSEPARQLGNLRGYLTSWSRDGTFGLATI
                     VNFDSDVRDIYRVSIPGGATEPWAVGPEPEYGANLSPDGRWVAYMVQRTGGDEAFVRP
                     LEGPGGPWSVAAGESGEPFWSRDGRLLYLQTERAVYAYAVGGGSQFELGAPRLLFRKR
                     MQETLCPAPDGRFLAILPNRTSGPRRVEVIRNWQALVTDEPRSK"
     gene            complement(6118..7734)
                     /locus_tag="VF720_05865"
     CDS             complement(6118..7734)
                     /locus_tag="VF720_05865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEX7878915.1"
                     /translation="MTLALSPRRRRAVTGGVMTAMFMAALEATVVSTAMPTVIGALGG
                     LAHYSWVFSAYLITATVTVPVWGKLSDLYGRRPLYQAGIALFLLGSILSGMSRTMGQL
                     IVFRAIQGLGAGAIVPVSMTIIADIYTLEERTRMQALFSSIWGISSILGPVAGGFITD
                     QLSWRWVFFINIPFGIAAAAIIGAAMREPEREKRPVIDYSGAALLMVSVTLLMFGLIE
                     GGGGMSRLLAPASLALFGGAALLFALFLQVERRAVDPIIPLSIFSNRVVSVSVVAGFL
                     AGVGMFGAITFIPLFAQGGLGVTATEAGSLLTPLLLSWVGLSVVGGKLLLRVGYRRTS
                     IAGFTLLTVGFVLLATFPASAPRAVLYLDLAVVGAGLGLSMLTLLIAVQQAVPRSDLG
                     TATSLNQFARSIGGAIGVAGMGVMLSSALAAHLGQAAQQGLIAADIAARLAHDPSALI
                     EPQAGAALSGTALQVLRASLTSAIHSVFWVAAAATVLALGVAFLLPGPARATPKREAC
                     TAEAGERMIMAELATIDPPNEPVESVGDRS"
     gene            complement(7800..8648)
                     /locus_tag="VF720_05870"
     CDS             complement(7800..8648)
                     /locus_tag="VF720_05870"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7878916.1"
                     /translation="MNRVAVIGGGTMGNGIVHVFAQHGHPVTLIEMSAELGQKALRTI
                     EGNFDRQVKKGVIAEDVKGGAIQRITTAVGPEAARDADLVVEAIVEDRAIKFELFRKL
                     DAICPPNAILASNTSSISITEIASQTKRPGQVIGMHFMNPVPMMKLVEIIRGHATSEA
                     TNDAIVELSTKLGKTPVEANDYPGFVANRVLMPMINEAIFALYEGVASAEAIDTVMKL
                     GMNHPMGPLQLADFIGLDTCLAIMNVLHDGLGDPKYRPCPLLKRMVAAGELGKKSGKG
                     FHTYGA"
     gene            complement(8685..10940)
                     /locus_tag="VF720_05875"
     CDS             complement(8685..10940)
                     /locus_tag="VF720_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7878917.1"
                     /translation="MTRAVLVLLGGLLAFGGRAAPVSAKSTPKEASALPSDTLITSYP
                     ESRVEDVVDTLHGTPVHDPYRWLEDDDSDAVRRWTANQNALTQSVLGGVPGRDYIRQR
                     LSELLKIGTIDAPKVRGQRLFFMARSGDQQQPVLYVKENGASRVLVDPNTLSGDGTTA
                     LDWWYPSPDGRLLAWGSSKNGDEWSTLRVRDVESGSDRIEAIPRTRAASVAWLPDGSG
                     FYYTRYPAVGDVPAGQENYHRRVFRHALDTDLAADSLVFGDGRAPEDWVDIQISKDGR
                     WLLASVSIGWNRTDLYLSDRTDSTGAWHTLAEGEPARYEGTFGNGDLFLWTNADAPRG
                     RLFRVDPAQADRANWTPVVPEASDALTTVSVIAGRLFLHYLHDASSRVVILNRDGLVE
                     STIALPVRGTVTAMTGEPDGTDAYVSFHSYFVPPVVYRCDPATGNLTTFENVQADIDS
                     SRYMLSQVFYPSKDGTRVSMFLVHRKGLKKNGSNPTLLYGYGGFNQNLVPGFQRNSFL
                     WLEQGGVLAITNLRGGGEYGETWHQGGMLDRKQNGFDDYLAAAQWLIKQKYTSSSKLV
                     AQGGSNGGLLVGAALVQRPDLFRAVVCQVPLLDMIRYHRFLVARLWTPEYGTSENPDQ
                     FGYLYRYSPYHNVKDGTAYPAVLLATAESDSRVAPLHARKMAARLQSASTSGRPVLLR
                     LETRAGHGVGKPVHKLVDEFTDVWSFVFSQLGITARAPVAAGPAAARTTKKAPAKPTA
                     KAKTTKKTSGR"
     gene            complement(10952..12136)
                     /locus_tag="VF720_05880"
     CDS             complement(10952..12136)
                     /locus_tag="VF720_05880"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7878918.1"
                     /translation="MSRDVVIVSACRTAIGRFQGGLAPLRAPEIGAVAVREAVSRAGI
                     APDAIEEVILGNVLQGGVGQAPARQAAHFGGIPDTVGSLTINKVCGSGLKAVMLAAQA
                     IKAGDAECVLAGGFESMSRAPYYLFGAREGFKMGHQQLVDGMIHDGLWDVYNNYHMGS
                     AAEFIAAKFKVTREDQDKFALASQLKAVAAMDAGKFKAEIVDVVIPQRKGDPVVVNTD
                     EGPRRDTTIEKLAALKPAFQKDGTVTAGNAPSVNDGAAALVVMSAERARVLGATPLAR
                     VTGYATGGTEPEMLFYAPVIAVRKLMKQMNVTIDHWDLIEANEAFSAQALVDGRELEW
                     DFDRVNVNGGAVALGHPIGASGARVLTTLLYALKDRGKKTGMATLCLGGGNAVALAVE
                     ML"
     gene            complement(12349..13065)
                     /locus_tag="VF720_05885"
     CDS             complement(12349..13065)
                     /locus_tag="VF720_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878919.1"
                     /translation="MRLFHRALLLILLAVTSFNVQNVQADPWDNDSKLLLHIRPPTQV
                     NPCGAHGALDDCRDAVTSAGVLTVPAEGPYYFVYLLVGKGSTPAGVAGVRVGVTYQGG
                     GGGGANDHVGVDVLAWRSCATLDEITPGLSVWPAPGSGITLTWDLPENCQEHATPVAG
                     YFYLGAYSADVMTLIGDPATGDATIRSCEADMIELNESQDLGSVAFNVPGSGCNPCDA
                     ACDDMPVLPATWGSIKTLLQ"
     gene            complement(13170..13868)
                     /locus_tag="VF720_05890"
     CDS             complement(13170..13868)
                     /locus_tag="VF720_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878920.1"
                     /translation="MNRSRWKSAIACIAWLTFPLSAARAANNPMILLHVVAANKVCGQ
                     AQLANCTAAISQGSLNTPYHVFVVVAPSDSMLQSGAGIAGLQFGLDYSGGFDPSGNPV
                     LPIDIFSWTLCATLEFITPTPQWPGPGGGNLITWDSVTNCQLGNLAVAGYFYLTAYSP
                     ATLRLTPRPVDQRAKVATCGAVELDTWNYYGLGIAAFSAGSTVPGCNPCIYPCGIDPV
                     ESTTWSRIKSQVGQ"
     gene            complement(13934..14638)
                     /locus_tag="VF720_05895"
     CDS             complement(13934..14638)
                     /locus_tag="VF720_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878921.1"
                     /translation="MRPILSILAPVIVVAALASCLIALPDARASTNDGKPKITLHVGG
                     ATVKNPCVHARLSDCMDAVTEGGLDIPYYAYLLVARGTQPSIAGAQFGIYYTGTYEPN
                     GVGQGVNVFGWTLCATLEFASPSPAWPSPGSGTLLTWDSVTDCQTGDVAVGGYFYLTA
                     YDPGIMFVGARPVDSVVKVATCEPFEYEILPGIDTGSAYFSSDLSAPGCNACMKSCID
                     PVEPATWSGIKAGGGR"
     gene            complement(14714..15670)
                     /locus_tag="VF720_05900"
     CDS             complement(14714..15670)
                     /locus_tag="VF720_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEX7878922.1"
                     /translation="MRHRPSLSAEPREDGLPARVDIVEVGPRDGLQNEKTLVPTEAKA
                     AFVRALAACGFPEIEVTSFVSPRWVPQMADASELLEAVGPPPSSVVYSALVPNEAGME
                     RAIAAGVKRVAVFTAASETFSRRNINASIAESIERFKPVMAMAKSAGISVRGYLSTAF
                     VCPYEGDIAPQAAGDVLRRLFELGVDEVSIGDTIGAASPLQVNDLLDELADDIDAGHL
                     AMHFHDTRGTALSNVLTSLERGIWIFDSSAGGLGGCPFAPGATGNLATEDLVYFLNRM
                     GIDTGVDLDAVRQASNALRAAATGVRQPSRVLEAMNAAPPRD"
     gene            15819..>16501
                     /locus_tag="VF720_05905"
     CDS             15819..>16501
                     /locus_tag="VF720_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878923.1"
                     /translation="MTPLSARWFVHACHHPAARLIALLIGLALFAGIGSARPRELGIP
                     SAHPDGLASEDGSAPFSDYHVSGLLSASDYDTSWYGGTVWAADSNRWEAIPGGIWTFD
                     SGVGSAIQTTPSPTKPVGYHRLMEGWTGLITGLTEPTDFRRMTTCVIAGSYSMWAGRT
                     LAEANARCWPGGQGYGNGVNTSLEQTFAYSGTGNVTLQFKYAVDAEPGFDDLSLLVRP
                     SGAANDVVLT"
BASE COUNT         2799 a         5406 c         5636 g         2660 t
ORIGIN      
        1 cgtagccggg ccagaggaag tcgccgtcgt tgttcttgcg gaaccagttc acctggtaga
       61 tgcgcgggag cttgttcggg tcgcccttgg ccccgatcga gagccagtgc ttgaagtaat
      121 cgcccatgtg gtagccgcag aaaggcagca tggcgaaggg gtcgtgacgg agctgcccga
      181 ccgtgccggc ggcggcggcg gtcatctcgc tccccatgat ggagcccagg aaggtgccgt
      241 gctgccagga gcgggcctcg ttgaccagcg gcaccacggt ggcacggcgg ccgccgaaga
      301 ggatggcgga gatcggcacg ccggccgggt tttcccactc cgacgagatc acggggcact
      361 ggcgggccgg cgtcgtgaag cgggcgttgg ggtgcgcggc ggggcggccg gccctgggcg
      421 tccaggggcg gcggagccag tccaccagcg tggcgggggg cgcgtcggtc atcttctccc
      481 accacacgtc gttgtcaggc gtgagcgcgc agttggtgaa gatcgagttc tcccgggcgg
      541 tgatcatggc gttggggttc gacttcatgc tggtgccggg ggccacgccg aagaagcccg
      601 cctcgggatt gatggcgtag aggcggccgt cctggccgta cttcatccag gcgatgtcgt
      661 cccccaccgt ctcgatcttc cagccgggga gcgtgggatt catcatggcc aggttcgtct
      721 tgccgcaggc cgaggggaac gccgccgcca cgtagatctt ctggcctttg ggatcggtga
      781 tgccgaggat cagcatgtgc tcggccatcc aaccctcatc gcgggcctgc acggaggcga
      841 tgcggagcgc atggcacttc ttgccgagga gcgcgttgcc gccgtagccc gagccgtacg
      901 accagatttc gcgcgattcc gggaagtgcg agatgtactt ggtcttggcg ttgcagggcc
      961 acgggctgtc gggagcgttg ccggtgatgg gcgagccgat ggagtgcatg cctcgcacga
     1021 actcgccgtt cgtgccgagc acatcgagca ccttcgtgcc gacgcgggcc atgatgtgca
     1081 tgttggccac cacgtagggg ctgtcggaaa tttccacgcc gatcttggcg atcggcgatc
     1141 caaccgggcc catgctgtac gggatcacgt acatcgtgcg gccggccatg cagccattga
     1201 agagcggcgt gagcgtggcc ttcatctcgg ccgggtcctt ccagttgttg gtcgggccgg
     1261 cgtcatcctt cgatttcgag cagatgaagg tgaactcctc gacacgggcc acgtcggccg
     1321 ggtcggaccg gacgaagatg ctgttcgggc gcctggccgg gtcgagccac tgggccgtgc
     1381 ccgtctgcac catcaggtgc agcatgcgct ggtactcctc gggcgagccg tcgcaccaga
     1441 ccacctggtc gggctggcag agggcggcgg tctccttgac ccaggaatcg agagcggaat
     1501 gcttcgtcat gatcgaacct tcaccgaggg aatgcgtcgg gagcgtcaag gcgccacgcc
     1561 cggccggggt ggcgcgaggg cgggaattct accgctcccg gcgcctccgg agaagccccg
     1621 cggggaggag gaggaagacc aggctttcca gcgggaaggt tggcccgaag cgccggtcgg
     1681 gccagtagac ctcctcgtcg gaatcggcca cgacgagggg gaggctccct tccagaacgt
     1741 cctggtaacc gtaggtccgc tccagtcggg tcaggaaacg tccgggggcc aggtagcccg
     1801 ccgcgagggg ctgcgattgg cccatcgcat cgagctcctt ttccgacaac tggttcgcgt
     1861 aattggtctg gtatccctcg agtgtccgcc gatggtcatc gaccacatag aagcgcaccg
     1921 ggaagaagtc ggagcggttg atgccgagaa ggtcgagcgg cacgtccact ctgtccgccg
     1981 cgtagcggaa ttccaccggg ttcacgttca cgaaccaccc gccgtccggg acctgcgatt
     2041 ccgggctctc ggggtcgagc ttcatggccg tgaagtacca cccgatcgac acgtagcggg
     2101 ccagcgcctg cgcctcggct tcggtcacct cgaagccgtt gctgaagagc cagctcacca
     2161 ggctgcgggg gtcggaggaa tggatggtgg tggcgatgaa gctgccgatc gagcgggtgg
     2221 cgatgatttc cgtgccggag ttgtcggccg gcgacggaga ttcatcaacc tggctgcatc
     2281 cgggacctcc gctactgccg ccgaaggagg tgacgggcgg ctgagtcagc gtggacagct
     2341 gtgaccagag cgccttgtcc accggcgcga gctcgggcac cgtcggcgtc ggcacgatca
     2401 gcgcgaagct tgcggccggg ccggtgatga ggatgttggg aatcagccgg acggtgcccc
     2461 cctcgcgatc gaacatgatg aaggtggcct gcccgctgtt gacgatctgg cactcatcgc
     2521 aggggaaaca ggagcacatg gcccgcgcac ccgacggaag gacgagcagc gcggccagca
     2581 tcaggagggt aacccggttt cgcatgatgt tcgtcctcac gatcagtgga gcccggtgct
     2641 gacgccgagt tcgagcgaca gcgcgctgta gttgatgtcg tcgagggctg cctgatagtc
     2701 cacgggttcc acgagcagga ggcgcgtgcg accgaaggcc cgccagtcgc tggccaggtg
     2761 gaattcggga cgcagctcga gcccggcccc cacggtcggc gtggaaaacg tctcctcgtt
     2821 gcggtcggtg aaccaggtcg gcatcaccgt gacaaagaat ccgacgaaca cgcggggcca
     2881 gctctcgcgg ctttcggggg ccaggcgcgt cgtgacgctc accgtgacgg gcaccagagt
     2941 gtagtgcgat tccggaaatt cgaacgtgga aatgtagcgc tcccccccac cggaatggac
     3001 gtagcccaca tcaagaccga ccatcgaagc cccgtccgcg aattccgtgg ccaggccgag
     3061 cccgaactgc ggcgcgatgc cgaaccgctc ccgctcgtcg ccgcccagcg gcgagtgaat
     3121 gccgccggat agtgacaggc gagcctcgcc cgcgcgggcc ggcggcgaga ggaaggccgg
     3181 gaagttgacg ggaacgagga gtggggcgag aacgaggggg aggacacgga tcggacggcg
     3241 gtgcggcatc gacgacctcc gggtgggttc cggccggtcg gagaccggcc cggcctgagt
     3301 gtagcgcgcc tcctacccgg ggcgacgggg tcggtttccg ccaattgagg cctggcgctg
     3361 gacagtgcgc accgtcacgt ggacactccc ccgcatgaca cccgagcggc tgggcggatt
     3421 ccggatcgac gaagagatcg gcgccggcgg catgggggtc gtgtatcgcg cgcacgacga
     3481 aaaactcggc cgcgcggtgg ccatcaaggt gctccccgag cgcgtggccg ctgatcccga
     3541 acggctggcc cgcttcgatc gcgaagcgcg cctcctggcc agcctcaatc atcccaatgt
     3601 ggccatcctt catggcctcg atcaggacgg ggacacgcgg ttcctcgtga tggagctggt
     3661 ggggggcgag tcgctcgaga agcggctccg gaacggggcg atgccgctcg cgaaggcgct
     3721 tcccatcgcg ctccaggttg catcggcgct ggaagcagcc catgcgcagg gaatcgttca
     3781 tcgcgacctg aagccgggca acgtgatgct cacgccggaa ggacgggtca agctcctcga
     3841 cttcggcctg gccaagatgc tctccgggga ttccggaagc tttcgtgaca gcggcacgcg
     3901 ggccaccacc gtgagcggct acctgatggg gacgcccggc tacatgtcgc ccgagcaggc
     3961 ttcgggcgat caggtcgacc gtcgcgccga cgtgtgggct ttcggctgcc tcctcttcga
     4021 gatggtgagc ggcaagcgcg cgttcggcgg cgaatcgctc acgcagatgc tgacggccat
     4081 cctctcggtc gatcccgact gggcgctcct ccccgccgac acgccggccg gcatccggcg
     4141 cctgctgcgg cgctgcctgg ccaaggatcc gaaccgacgt ttgcacgaga tggcggacgc
     4201 acggatcgag atcgaagaga cgctggcgcc gccgtcgggg gccaccgggg agtttgccgt
     4261 ggtcgggaga gcggaatcga tagcggactc gcccggccac ggacgcgcca gcccgtcgcc
     4321 ggtggcgacg cgcgcggccg gggtgccgcc ctggctggta atcgccggac tcttgctcgt
     4381 catcgtgctg gccgggatgg cgatgccgct cctgatgaag gggagcgggc ggcaggccgg
     4441 ccgcgaacct ggcccgcgtc gctactccat cgcgatgccg gattcgatga acctcgccgg
     4501 cgactatcgg cccacggtcc gcatttcgcc cgatggtcgg tggctggcgg cgtcggtgta
     4561 cacggccgac tggcaacgcc ggacgctgat cgaggacctg gtgaccgggg ccgtgcggat
     4621 agtggcacgg ccggatgtgc tcaccctcgg cttctccccc gacagccgtg aggcgtacat
     4681 ggagacgcgc gacgcccgct gggtggagga actgtccacc ggcacgctgc ggccgttgcg
     4741 tcatgacgac atcgaggcca ccccgctcgg catcatctgg atcgacgatg gtttcatcgt
     4801 ccagcccagc ttcggacgac cgttgctgcg tttgaaagcc gacggttcct cgctgcgaag
     4861 cctggcgccg atcgacagca gcaccggcga gatctgccat ctcgatcccc ggcggatacc
     4921 gggcaccgac tggatcctct tcaccgtctt ttcgggcggg gaatggactt ccgcccggat
     4981 cgaggcgatg aacttgacca ccggggaacg tcggcgcgtg ctggacggcg cgatcggcgg
     5041 tcgcgccacg gccgacgggt ggttgcttca tctcagaaac acggacctgc tggcgatgcg
     5101 gttcgatccg aagcggatga ccgtgtccgg gacggcgcga acggtggccc acgacgtgca
     5161 gttcttcccg acgaccggcc tcgcgtcgta tgacgtgtcc gacgacggca ccctggtgta
     5221 cgtgaccggc ggcgtggccg acatcaggga aacgttcacc tggctggatg aggcggggaa
     5281 tgaaacaccc acggccatcc cccccggggg ctacgccgac gccagcctct cccccgacgg
     5341 cacacgcgtg gcgatgaccc tggaaggcac caggtaccag atcggcattg cgaagatcgc
     5401 ggggggaccc gtgagggagt tcacgttcaa cagcgacgac aactatgcca gatggtcgcc
     5461 cgacgggcgg atgctcgcgt acacgggtga ctcgccgcgc cgcgccgtca tgctccgtcc
     5521 cgtggacggc agtgagccgg cccggcaact gggaaatctc aggggctatc tcacgagctg
     5581 gtcccgggac ggcacgttcg gcctggccac gatcgtcaat ttcgactccg acgttcggga
     5641 catctaccgg gtgtcgattc ccgggggagc caccgagccc tgggccgtcg ggccggaacc
     5701 ggagtacggc gccaacctgt ccccggatgg ccggtgggtg gcctacatgg tgcaacgcac
     5761 cggcggcgac gaggccttcg tccgtccact ggaaggcccc ggcggaccgt ggtcggtggc
     5821 ggccggcgag tccggcgaac cgttctggtc gcgggacggc cggctcctgt acctccagac
     5881 ggagcgggcc gtctacgcgt acgcggtcgg cggcggcagc cagttcgagc tgggggcgcc
     5941 gcgcctcctc ttccggaagc gcatgcagga gacactctgt ccggctcccg acgggcgatt
     6001 cctggccatc ctgccgaacc ggacttccgg cccgcgccgc gtggaggtca tccgcaactg
     6061 gcaggcactc gtcacggacg agccccggtc gaagtagcgc cccgggaccg gtcgatttca
     6121 gctccggtcg cccaccgact ccaccggctc gttcggggga tcgatcgtgg ccagttccgc
     6181 catgatcatc cgctcgccgg cttccgcggt gcacgcttcc cgcttcggcg tcgcgcgggc
     6241 cggccccggc agcaggaacg ccacaccgag cgccaggacg gtggcggcgg ccgcaaccca
     6301 gaagacggaa tggatggccg acgtcagcga cgcgcgcaac acctggagcg cagtgccgct
     6361 caacgcggcg ccggcctgcg gctcgatgag ggcgctcggg tcgtgcgcca gcctggcggc
     6421 gatgtccgcc gcaatgaggc cctgttgcgc ggcctgtccg aggtgggccg cgagggcgct
     6481 cgacagcatg acgcccatcc ccgccacacc gatcgcgccg ccgatcgagc gggcgaactg
     6541 gttgagcgag gtggcggtgc cgaggtcgga gcgcggcacc gcctgctgca cggcaatgag
     6601 gagagtcagc atggagaggc cgaggcccgc gccgaccacc gcgaggtcga ggtagaggac
     6661 ggcacgcggg gcggatgcgg gaaacgtggc cagcagcacg aagccgacgg tcagcagcgt
     6721 aaaaccggcg atgctggtcc ttcgatagcc cacacgcagc aggagttttc cgccgacgac
     6781 cgacaggccg acccaggaga ggaggagcgg cgtcagcagc gaacccgcct cggtggcggt
     6841 aacgccgagt ccgccctggg cgaagagcgg gatgaaggtg atcgccccga acatccccac
     6901 gccggccagg aagccggcca ccaccgacac gctcacgacg cggttcgaga agatcgagag
     6961 ggggatgatc gggtcgaccg cgcgcctctc gacctgcagg aagagcgcga agaggagggc
     7021 ggcgccgccg aagagggcga ggctggccgg cgcgagtagc cggctcatcc ctcctccacc
     7081 ctcgatcagg ccgaacatga ggagcgtgac cgacaccatg aggagcgcgg cgcccgaata
     7141 gtcgatcacc ggccgcttct cgcgctcggg ctcccgcatg gcagccccga tgatggccgc
     7201 cgcggcgatc ccgaagggga tattgatgaa gaagacccag cgccacgaga gctggtcggt
     7261 gatgaagccg ccggccaccg gccccaggat gctcgaaatt ccccagatgc tcgagaagag
     7321 ggcctgcatg cgcgtccgtt cctcgagcgt gtagatgtcg gcgatgatcg tcatgctgac
     7381 cggcacgatg gccccggcgc cgagtccctg gatggcccgg aagacgatca gctgccccat
     7441 cgtgcgcgac atgccggaaa gaatcgaccc gaggagaaag agcgcgattc ccgcctggta
     7501 caacggccgg cggccgtaca ggtcggagag ctttccccag accggaacgg tgacggtggc
     7561 ggtgatgagg taagcggaaa aaacccagct atagtgggcg aggccgccga gcgcgccgat
     7621 gacggtcggc atggcggtgc tcacgacggt ggcctcgagc gccgccatga acatggctgt
     7681 catgaccccg ccggtaacgg ctcggcggcg gcgaggggag agggcgagag tcatgtggat
     7741 tgagcttctc aactccgccg atgggaagca acacagcgac ctgatttacg ggaatgcccc
     7801 tatgccccgt acgtgtggaa tcccttaccg ctcttcttcc ccagctcgcc cgcggccacc
     7861 attctcttga gaagcggaca cgggcgatac ttcggatcgc cgagcccgtc gtgcagcacg
     7921 ttcatgatgg ccaggcacgt atcgagcccg atgaaatcgg ccagctggag cggccccatc
     7981 ggatggttca tcccgagctt catcacggtg tcgatggcct cggcgctggc cacgccttcg
     8041 tacagggcga agatggcttc gttgatcatc ggcatcagga cgcggttggc cacgaagccg
     8101 ggatagtcgt tcgcctccac cggcgtcttg ccgagcttcg tcgagagctc gacgatggcg
     8161 tcattggtgg cctcgctggt ggcgtggccg cggatgatct cgacgagctt catcatcgga
     8221 accgggttca tgaagtgcat gccgatcacc tggccggggc gcttcgtctg gctggcgatc
     8281 tcggtgatgg aaatggacga cgtattcgag gccaggatgg cgtttggcgg gcagatggcg
     8341 tcgagcttcc ggaagagctc gaacttgatc gcgcggtcct cgacgatggc ttccacgacg
     8401 aggtcggcgt cacgcgccgc ttcgggcccc accgccgtcg tgatgcgctg gatcgcgccc
     8461 cccttcacgt cctcggcgat gacacccttc ttcacctggc ggtcgaagtt cccctcgatc
     8521 gtcctcaggg ccttctggcc cagctcggcg ctcatctcga tgagcgtgac ggggtgcccg
     8581 tgctgcgcga agacgtggac gatgccgttt cccatggtgc cgccgccgat gacggccacg
     8641 cggttgatcg actgcgacat gacttcctcc aggtaccggg cgcgtcagcg accggaagtc
     8701 tttttcgttg tcttggcttt cgccgtcggt ttggcgggcg ccttcttcgt ggtcctggcg
     8761 gcggcgggcc cggccgccac gggcgccctg gccgtgatgc cgagctgcga gaagacgaac
     8821 gaccagacgt cggtgaactc gtccaccagc ttgtgaacgg gtttccccac gccgtggccg
     8881 gcccgtgtct cgagtcgaag cagcacaggg cgtcccgagg tgctcgcgga ctggagccgc
     8941 gccgccatct tgcgcgcgtg cagcggcgcc acgcggctgt cggactcggc ggttgccaac
     9001 agcaccgccg gataggcggt gccgtccttc acgttgtgat acggcgagta tcgatacagg
     9061 tagccgaact ggtcgggatt ctccgacgtg ccgtactccg gagtccatag tcgcgccacc
     9121 aggaaccggt gatagcggat catgtcgagc agcggcacct ggcataccac cgcacggaag
     9181 aggtcggggc gctgcaccag cgccgcgccg accagcaagc cgccgttcga gcccccctgc
     9241 gccaccagtt tcgaggacga ggtgtacttc tgcttgatga gccactgggc ggccgccagg
     9301 tagtcgtcga acccgttctg cttcctgtcg agcatccccc cctggtgcca tgtctcgcca
     9361 tattcaccac caccgcgcag gttggtgatg gccagcacgc ctccctgctc gagccacagg
     9421 aacgagttgc gctggaagcc cggcaccagg ttctggttga agccgccata gccatagagg
     9481 agcgtcgggt tggaaccgtt cttcttcagc cccttcctgt gcaccaggaa catcgacacc
     9541 ctggtgccgt ccttcgaggg gtagaagacc tggctcaaca tgtagcggct cgaatcgatg
     9601 tcggcctgca cgttttcgaa cgtcgtgagg ttgccggtag ccgggtcgca gcgatagacg
     9661 accggcggaa caaaatagga gtggaacgac acgtaggcgt ccgtgccgtc cggctcgccc
     9721 gtcatggccg tcaccgttcc ccgcaccggc aacgcgatgg tgctctccac aaggccatca
     9781 cggttcagga tgaccacgcg gctcgaggca tcatgcaggt aatgcaggaa aagtcgcccg
     9841 gcgatgaccg aaaccgtggt gagcgcatcc gaagcttcgg gaaccacggg cgtccagttc
     9901 gcccgatcgg cctgcgcggg atcgacccgg aacagacggc cgcgcggtgc gtcggcgttg
     9961 gtccagagga agaggtcgcc gtttccgaac gtgccctcgt agcgcgccgg ctctccttcg
    10021 gccagcgtgt gccaggcccc ggtcgagtcg gtgcggtccg acaggtagag gtcggtccgg
    10081 ttccacccga tcgataccga ggccaggagc cagcgtccat ccttcgagat ctggatatcc
    10141 acccagtcct cgggcgcgcg cccgtcgccg aacaccagcg aatccgcggc gagatcggta
    10201 tcgagcgcgt gccggaaaac gcgccggtga tagttctcct gtccggcggg cacgtctccg
    10261 accgccggat agcgggtgta gtagaagccg gagccatccg gcagccaggc caccgaggcg
    10321 gcccgtgtcc gcgggatggc ctcgatccga tcactgcccg attcgacatc acgcacgcgg
    10381 agcgtgctcc actcgtcccc attcttcgac gaaccccatg ccaggaggcg cccatccggc
    10441 gagggatacc accagtcgag cgcggtcgtg ccgtcgccgg agagcgtgtt gggatcgacc
    10501 aaaacccggg aggcgccgtt ctccttcacg tagagcaccg gctgctgctg gtcgccgctc
    10561 cgtgccatga agaagaggcg ctgcccgcgc accttgggtg cgtcgatcgt accgatcttc
    10621 aggagttcgg aaagacgctg gcggatgtag tcgcggcctg gcacgccgcc gagcaccgac
    10681 tgcgtgagcg cgttctggtt ggcggtccag cggcgcaccg cgtcggaatc gtcgtcctcg
    10741 agccagcgat aggggtcatg aacgggcgtc ccgtgcaagg tatcgaccac gtcctcgacg
    10801 cgggattcgg gatacgacgt gatcagcgta tcggacggca aggcgctggc ctctttcggt
    10861 gtggatttcg cagaaacggg agcggcccgc ccgccgaatg cgagcaggcc gcccagcagg
    10921 acgagtacgg cccgggtcat gaccgggtcg attacagcat ttccacggcc agtgccaccg
    10981 cgttgccacc gcccaggcag agcgtggcca tgccggtctt cttgccgcga tccttgagcg
    11041 cgtataggag tgtcgtgagc acgcgcgccc ccgaggcgcc gatcgggtgc cccagcgcca
    11101 cggcaccgcc gttcacgttc acgcggtcga aatcccactc gagctcacgg ccatcgacca
    11161 gcgcctgggc actgaaggcc tcgttggcct cgatcaggtc ccagtggtcg atcgtcacgt
    11221 tcatctgctt catcagcttg cgcaccgcga tgaccggcgc gtagaaaagc atttcgggct
    11281 cggtaccgcc ggtggcgtag ccggtgacgc gcgccaaagg cgtggcaccc aggaccctgg
    11341 cccgctcggc cgacatcacg accagcgcgg cggccccgtc gttcacggag ggcgcgttcc
    11401 cggccgtgac ggtgccgtcc ttctgaaagg ccggcttcaa cgccgccagc ttctcgatgg
    11461 tggtgtcgcg ccgggggcct tcgtccgtgt tcaccaccac cggatcaccc ttccgctgtg
    11521 ggatcaccac gtccacgatc tcggccttga acttgccggc gtccatggcg gccacggcct
    11581 tgagctggct cgcgagcgcg aacttgtcct ggtcctcgcg cgtcaccttg aacttggcgg
    11641 cgatgaactc ggccgccgag cccatgtgat agttgttgta cacgtcccac agcccgtcgt
    11701 ggatcatgcc gtccacgagc tgctggtgcc ccatcttgaa accctcgcgc gcgccgaaca
    11761 ggtaatacgg ggcccggctc atgctttcga agccaccggc caggacgcac tccgcatccc
    11821 ccgccttgat ggcctgggca gccagcatca ccgccttgag ccccgagccg cacaccttgt
    11881 tgatggtgag tgagccgacg gtgtcgggga tgccgccgaa gtgggccgcc tggcgcgccg
    11941 gcgcctgtcc cacgcccccc tgcagcacgt tgccgaggat cacttcctcg atggcatccg
    12001 gggcgatgcc ggcacgcgac acggcttctc gcacggccac ggcgccgatt tcgggcgcac
    12061 ggagcggggc cagccctccc tggaatcggc cgatggccgt ccggcaggca ctgacgatca
    12121 cgacatcgcg ggacatgggg ttacctcctt caacgaggcc cggggcgggc ccctcacgat
    12181 cgatttggag tcgggcggaa cgtctgccga ttcgcgccga aacacgcgga aaacgaccca
    12241 gagcctaccg cagggcggtt ttccgctcaa cagaggaggg cggcgtacgc cgcccgttcc
    12301 tcatgctgtc aagtcgtggg ggtttgccgc ctcgctggcc atccctgcct attgcaggag
    12361 cgtcttgatg ctcccccagg tggccggcag caccggcatg tcgtcgcagg cggcatcgca
    12421 cggattgcac cccgagccag gaacgttgaa ggccaccgaa cccaggtcct gcgattcatt
    12481 caactcgatc atgtcggctt cgcagctccg gatcgttgca tcgccggtgg cgggatcgcc
    12541 gatcaaggtc atgacatccg ccgaataggc cccgagatag aaatagccgg cgaccggtgt
    12601 cgcatgctcc tggcagttct cggggaggtc ccatgtaagg gtgatcccgc tcccaggcgc
    12661 cggccagaca gacaggccgg gcgtgatctc gtccagcgtg gcacacgacc gccatgccag
    12721 cacgtccacg ccgacatggt cattggcccc cccacctccg ccaccctggt aggtgacgcc
    12781 cacgcggact cccgcaacgc cggccggcgt cgaaccttta ccgacgagca gatagacgaa
    12841 gtagtagggc ccctcggccg gcacggtcag aaccccggca ctcgtcaccg catcgcggca
    12901 gtcatcgagc gcgccgtggg ccccgcatgg gttcacttgc gtggggggac gtatatgaag
    12961 gaggagcttg gagtcgttgt cccagggatc agcttgcacg ttttgaacgt tgaatgacgt
    13021 aactgccaac aggataagga gcagcgcgcg gtgaaacaat cgcatggcga cctccagggt
    13081 aaagacgtcc ccgcacgacg gggcagccct gggccagacg tgtatcacta tgacacgaag
    13141 aagtgactca tccgcaaccg ggggatcccc tactgaccaa cctgggactt gatgcggctc
    13201 caggtggtgg actcgacggg gtcgatgccg caggggtaga tgcagggatt gcatcccggg
    13261 acggtgcttc cggcgctgaa ggcggcaatg cccagcccgt aataattcca cgtgtcgagc
    13321 tcgaccgccc cgcaggtggc caccttggcc cgctggtcca ccggccgcgg agtcaaccgc
    13381 agcgtggccg ggctgtaggc cgtcaggtag aagtaaccgg cgaccgcaag attgcccaac
    13441 tggcagttgg tgacggaatc ccaggtgatc aggttgcccc cgccgggccc cggccactgc
    13501 ggggtcggcg tgatgaattc aagggtggca cagagcgtcc aggagaaaat gtcgatcggc
    13561 aacaccggat ttccggacgg atcaaatcca ccggagtaat ccaggccgaa ctgcaggccg
    13621 gcaatccccg caccggactg aagcatcgaa tcggaaggcg caaccaccac gaacacgtga
    13681 taaggcgtgt tcaggcttcc ctgggagatc gcggcggtgc aattggccag ttgcgcctgc
    13741 ccgcacacct tgttggccgc caccacgtgc agaagaatca tcggattgtt cgcggcgcgc
    13801 gcggccgaga gcgggaaggt cagccacgca atacaggcga tcgcggactt ccatcgtgaa
    13861 cgattcatgc gtacctcccg gaggccgttc caagttcatg catggagtgc cggaggtgcc
    13921 agaaattcgg aggtcagcgg ccgccccccg ccttgatccc gctccatgtc gcgggctcca
    13981 ccgggtcgat gcatgacttc atgcaggcgt tgcacccggg agcggagaga tcgctgctga
    14041 agtaggcact ccccgtatcg atgccgggca ggatctcgta ttcgaacggt tcgcaggtgg
    14101 ccaccttcac gacactgtcc acggggcgcg caccgacgaa catgatcccc ggatcgtagg
    14161 cggtcaggta gaagtagcca cctaccgcca cgtcgccggt ctggcaatcg gtcacgctgt
    14221 cccaggtgag cagcgtgccg ctcccgggcg agggccaggc gggcgaggga ctggcaaact
    14281 cgagcgtggc gcagagcgtc cagccgaaga cgttgactcc ctggccgacg ccgttgggtt
    14341 cataggtccc cgtgtagtag atgccgaact gcgcgcccgc aatgcttggc tgcgtgcctc
    14401 tggccacgag caggtaggcg taatagggaa tgtccaggcc accctccgtg acggcatcca
    14461 tgcagtcgga caacctcgcg tggacgcagg gattcttcac agtggcgccc cccacgtgga
    14521 gggtgatttt gggcttgccg tcgttcgtgg acgcgcgcgc atcgggcagg gcaatgaggc
    14581 atgacgcgag tgcggcaacc acgatgacag gggcaaggat cgaaaggatc ggtctcacga
    14641 atgatcctcc aggtgagaga tgcccgggaa cccgttccaa gtgtatgcgc aaagtcgcgg
    14701 atcggccacg ccgtcagtcg cgtggcggag ccgcgttcat cgcctccagg acccgggaag
    14761 gctgccttac ccctgttgcc gccgctcgca acgcgttgga tgcctgccgg accgcgtcca
    14821 ggtcaacgcc cgtgtcgatc cccatccgat tcaggaagta aacgagatcc tcggtggcca
    14881 ggttgcccgt ggccccgggg gcaaaggggc agccgccgag cccgccggcc gaggagtcga
    14941 agatccagat gccgcgctcg agcgacgtga ggacgttgga gagggcggtg ccgcgcgtgt
    15001 cgtggaagtg catggccagg tggccggcgt cgatatcgtc ggccagttcg tcgaggaggt
    15061 cgttgacctg gagcggcgag gcggcgccga tggtgtcgcc gatcgatact tcatcgacgc
    15121 ccaactcgaa gagccggcgg agcacatcgc ccgcggcttg cggcgcgatg tcgccttcgt
    15181 aagggcagac gaacgccgtc gagaggtagc cgcgcacgct gatgccggcc gacttcgcca
    15241 tcgccatgac cggcttgaac cgctcgatcg attcggcgat ggaggcattg atgttcctcc
    15301 ggctgaacgt ctcggaggcc gcggtgaaga ccgccacgcg tttcacgccc gccgcgatgg
    15361 cccgttccat cccggcctcg ttcggcacga gcgccgagta gaccacactc gaagggggcg
    15421 gcccgaccgc ctcgagcagc tcggaggcat ccgccatctg cggtacccac ctgggactga
    15481 cgaacgacgt cacctcgatc tcgggaaagc cgcacgcggc cagggcgcgc acgaacgccg
    15541 ccttggcttc cgtcggcacg agcgtcttct cgttctggag cccgtcccgc ggcccgactt
    15601 ccacgatgtc gacgcgcgcg ggaagtccgt cctcgcgggg ttcggcggac aggctggggc
    15661 ggtggcgcat ggtcggtgct ctcctcgggc aattaccaga tgcgacgcag cttacaggat
    15721 gcgaattgcc accgcgacga tcaattcgac agggccgaaa ccggaaggcg tccgtatact
    15781 gaatgagtca ccgatttcct cggcccagga gaacagcgat gacccccctc tccgcacgct
    15841 ggtttgttca cgcctgccat caccccgcag cccggctcat cgccctcctg atcggtctgg
    15901 ctcttttcgc cggtatcggc agtgcccgtc cccgcgaact gggaattccc tcggcgcatc
    15961 ccgacggcct ggcctccgag gacggctccg ctccattttc cgactaccat gtttcggggc
    16021 tcctttcggc ttccgactac gacacgagct ggtacggcgg cacggtctgg gccgcggact
    16081 cgaaccggtg ggaggccatc ccgggcggca tctggacgtt cgacagcggc gtgggatcgg
    16141 ccatccagac cacgccgagc ccgaccaagc ccgtcgggta ccaccgcctc atggaagggt
    16201 ggacgggcct catcaccggg ttgaccgaac cgaccgactt ccgccggatg accacgtgcg
    16261 tcatcgccgg aagctactca atgtgggccg gccggacgct ggccgaagcc aacgcccgtt
    16321 gctggccggg tggccaggga tacggcaacg gcgtgaacac gagcctcgag cagacgtttg
    16381 cgtacagcgg cacgggaaac gtcaccctgc agttcaaata cgccgtggac gccgaaccgg
    16441 gcttcgacga cctctcgctg ctcgtacgac cctcgggagc ggcgaacgac gtcgtgctga
    16501 c
//
LOCUS       DASVOD010000268         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1010930, whole genome shotgun sequence.
ACCESSION   DASVOD010000268 DASVOD010000000
VERSION     DASVOD010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010930"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="VF720_05910"
     CDS             <1..1192
                     /locus_tag="VF720_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878924.1"
                     /translation="EAMSAYRSGDMLKYRDRLAAADSLLPGQPALRRRLAGAEARLGH
                     PDAAARWLNRFSSMNVRFDLAQDKDLESIRSRPDYMVALARQAELEKPVRSSITGAEI
                     PEKQLLTEGIAFDPRPRRLFISSVHKRKILTFTDGRWGTLAPARGESLLAPLGMTFDG
                     DNNSLWVATTAMAEMTGYDSTMEGRTELRRLDLESGRSLGTWVVPDTGRDHNLNDLVL
                     DGRGRLYVSDSVSGAIYRLESAEHDHLEIFLPRGTFGSPNGLAISRGDSLLYVSDYTL
                     GVYVIDVKTKGIERLAAPDSLCLNGVDGLVGFDGHLVMIQNGIVPHRLVRAGLDATGR
                     RIRDVVTLDRAHPSWDEPTLGVLDGQEMFYIARSQWGKMLPGGGMPPADSLDLPIVMR
                     VRVR"
     gene            complement(1211..>1783)
                     /gene="mraW"
                     /locus_tag="VF720_05915"
     CDS             complement(1211..>1783)
                     /gene="mraW"
                     /locus_tag="VF720_05915"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX7878925.1"
                     /translation="FDGPLDMRMDRRGKVTAATLVASLSEEELSELLRRHGDEPEHVR
                     IARKIVERRAIEPITRTRQLAYLVLRTLKMTPDEWKEHADRNPGARHPAARTFQALRM
                     AVNDEIGQLEHLLRTVPWCLKPGGRVVILSFHSGEDGRVAEAFEKGLADGTWSEITTE
                     AIRPLVAERRDNPRAASARLRRAVRAPSSE"
BASE COUNT          271 a          605 c          612 g          295 t
ORIGIN      
        1 ggaagcgatg agtgcctatc gctcgggcga catgctgaaa tatcgtgacc ggctggcggc
       61 cgccgattcg ctccttccgg ggcagccggc gttgcgccgg cggctggccg gtgcggaggc
      121 gcgcctcggt catccggatg ccgccgcgcg gtggctcaac cggttttcct cgatgaacgt
      181 ccggttcgac ctggcgcagg acaaggacct ggagagcatc cgaagcaggc cggattacat
      241 ggtggcgctg gcccggcagg ccgagctcga gaagcccgtc aggagttcga tcacgggcgc
      301 ggagatcccg gagaagcagc tcctcacgga ggggatcgcc ttcgatccac gcccccgccg
      361 gctcttcatt tccagcgtgc acaagcggaa gatcctcacc ttcaccgatg ggcgatgggg
      421 aacgctggcg ccggcccgcg gcgaatcgct cctggcgccg ctcggcatga cgttcgacgg
      481 cgacaacaac tcgctctggg tggccacgac cgccatggcc gagatgaccg gctacgactc
      541 caccatggaa gggcggaccg aattgcgccg cctcgacctc gaaagcggga gaagcctcgg
      601 cacctgggtc gtgcccgaca ccggccgcga tcacaacctc aatgacctcg tcctcgacgg
      661 ccgcggccgg ctttacgtct ccgacagcgt gagcggcgcc atctaccgcc tcgaaagcgc
      721 cgagcacgat cacctcgaga tattcctgcc gcgtggcacg ttcggatcgc cgaacggcct
      781 ggccatctcc cggggcgatt cgctgctcta cgtgtcggac tatacgctcg gcgtctacgt
      841 gatcgacgtg aaaacgaagg ggatcgaacg gttggccgcg cccgacagcc tctgcctgaa
      901 cggtgtcgat ggcctcgtcg gcttcgacgg gcacctcgtc atgatccaga acggcatcgt
      961 gccgcatcgc ctggtgcgcg ccgggctcga tgccacgggc cgccgcatcc gggatgtcgt
     1021 cacgctcgat cgcgcccacc cctcatggga tgaaccgaca ctgggggtgc tcgacggcca
     1081 ggagatgttc tacatcgccc gcagccagtg ggggaagatg cttcccggag gcgggatgcc
     1141 acccgccgac tcgctggatc tgccgatcgt gatgcgggtg cgggtgcgct aggctggtcg
     1201 tccgcccgca ctactcgctc gagggagccc gcaccgcccg acggagtcgc gccgatgccg
     1261 cccgcggatt gtcgcgccgt tccgccacga ggggtcggat cgcttcggtc gtgatttcgc
     1321 tccaggtacc gtcggccagc cccttctcga aggcctcggc cacgcgcccg tcctcgccgc
     1381 tgtggaacga gaggatcacc acgcgccctc ccggcttcag gcaccaggga accgtgcgga
     1441 ggagatgctc gagctggccg atctcgtcgt tgacggccat ccggagggcc tggaacgtgc
     1501 gggcggcggg atggcgggcg cccgggttcc tgtcggcgtg ttccttccac tcgtccgggg
     1561 tcatcttgag cgtgcgaagg accaggtagg cgagctgcct tgtgcgggtg atgggctcga
     1621 tcgcgcggcg ctccacgatc ttcctggcga tgcggacgtg ttccggttcg tcgccgtggc
     1681 ggcgaagaag ctcggagagt tcctcctcgc tgagcgaggc caccagggtg gcggccgtca
     1741 ccttgccgcg ccggtccatg cgcatgtcga gcgggccgtc gaa
//
LOCUS       DASVOD010000269         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_539043, whole genome shotgun sequence.
ACCESSION   DASVOD010000269 DASVOD010000000
VERSION     DASVOD010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539043"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2091)
                     /locus_tag="VF720_05920"
     CDS             complement(<1..>2091)
                     /locus_tag="VF720_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX7878926.1"
                     /translation="VIPPNVNMAIAGGILILTPRPKDRGPHLPVDYWFRSLAEDQKGR
                     AIGVVLSGTGSDGTLGLCEIKAVGGITFAQDESTARHAGMPQSAIHSGSVDFVLSPEA
                     IASRLKEIGQHPYLAPVSMDDRTPEDTDHQFKRILGAIRASTGVDFSLYRDTTIKRRI
                     MRRMALHRLKTWEEYRQFLQDTPAEIEDLYHDLLINVTSFFRDAEMFEALKVHVFPEI
                     TREKSPSTPIRMWVPGCSTGQEAYSLAMSLLEYYDDKPITPAIQIFATDLSDQKSLDR
                     ARAGVYPESIEAVVSPERLRRFFRREDHVYRINKNIRDSCVFARQNVTSDPPFSHLDI
                     ISCRNVLIYLSTPLQKRVLPTFHYALNAPGYLLLGTAETVGENADLFELVDRSHKIYA
                     KKGTAARLPLHYATEGMRGVDMPNTRRFGPLNQVPSDFQREADRMLLGRYAPAGVLIN
                     ENLDILQFRGRTSAYLEPAPGEPTTNVLKMAREGLFMELRSALTEARKHQRAVRREKV
                     RITADGQAHDVTVEVVPIKPPGAAEGCFLILFHDGEREGARGPSTVPQDKASPGREMP
                     DDGDAASLRQELAATKEYLQSLAEQQDAANEELRSANEEILSSNEELQSTNEELETAK
                     EELQSANEELTTVNEQLQHRNHELSRLNNDLTNLMASTHIPVVMVSSDLRIRSFTGPA
                     RKIMSLLPTDVGRP"
BASE COUNT          318 a          647 c          711 g          415 t
ORIGIN      
        1 atgggccggc cgacgtcggt ggggaggagg ctcatgatct tgcgggccgg cccggtgaag
       61 ctgcggatgc gaaggtcgct gctgaccatc acgaccggga tgtgcgtcga ggccatcagg
      121 ttggtgaggt cgttgttgag gcggctcagc tcatggttcc ggtgctggag ctgctcgttg
      181 acggtcgtga gctcctcgtt ggccgactgg agctcctcct tggccgtctc caactcctcg
      241 ttcgtgctct ggagttcctc gttgctggag aggatttcct cgttggccga acgaagctcc
      301 tcgttggcgg catcctgctg ttcggccagc gactggagat attccttcgt ggccgccagt
      361 tcctggcgaa gactggcggc atctccgtcg tcgggcattt cccgcccggg cgaggccttg
      421 tcctggggta ccgtggatgg gcccctggcc ccttcacgtt cgccatcgtg gaacaggatg
      481 agaaaacacc cttcggccgc gcccggcggc ttgatcggaa cgacctccac ggtcacgtcg
      541 tgggcctgcc cgtccgcggt gatccgcacc ttttcccgcc ggacggcgcg ctggtgcttc
      601 ctggcttcgg tgagcgcgct ccggagttcc atgaagagcc cctcgcgggc catcttgagc
      661 acgttggtgg tgggttcccc gggagccggc tcgaggtagg cgctggtgcg tccgcgaaac
      721 tgcaggatgt cgagattctc gttgatcagc acgccggccg gcgcgtatcg ccccagcagc
      781 atccggtcgg cctcgcgctg gaagtcggac ggcacctggt tcaacggacc gaaccgacgc
      841 gtattcggca tgtccacacc gcgcattccc tcggtggcgt aatgcagcgg caggcgggct
      901 gccgtcccct tcttggcgta gatcttatga cttcggtcga cgagctcgaa gaggtcggcg
      961 ttctctccca ccgtctcggc cgtcccgagc agcaggtagc ccggcgcgtt cagcgcgtaa
     1021 tgaaacgtcg ggagaacacg cttctgcaag ggcgtggaga ggtagatgag cacgttccgg
     1081 caactgatga tgtcgagatg cgagaagggc ggatccgagg tgacgttctg acgcgcgaag
     1141 acgcagctgt cccggatgtt cttgttgatc cggtagacgt ggtcctcccg ccggaagaac
     1201 cgccggagtc gttcggggct caccaccgcc tcgatgctct ccgggtacac gccggcccgc
     1261 gcccgatcca gcgatttctg gtcgctcagg tcggtggcga agatctggat ggcgggcgtg
     1321 atcggcttgt cgtcgtagta ctcgagcagc gacatggcca gcgaataggc ttcctggccg
     1381 gtggagcacc ccggcaccca catccggatt ggcgtggacg ggctcttctc ccgcgtgatc
     1441 tcggggaaga catgaacctt gagcgcctcg aacatctccg cgtcacggaa gaagctggtg
     1501 acgttgatca ggaggtcgtg atagaggtcc tcgatctccg ccggagtgtc ctggaggaac
     1561 tgccggtatt cctcccatgt cttcagccgg tgaagcgcca tgcgtcgcat gatgcggcgc
     1621 ttgatcgtgg tgtcgcggta caggctgaag tccacaccgg tcgacgcgcg gatcgcgccg
     1681 aggatccgct tgaactggtg atcggtgtcc tcgggcgtgc gatcgtccat cgacacgggg
     1741 gccaggtagg gatgctggcc gatttccttc aacctcgatg cgatggcctc gggcgagagc
     1801 acgaaatcca cgctgccgct gtggatggcg ctttgcggca tgccggcatg cctggccgtc
     1861 gattcatcct gggcgaaggt gatgccgccg accgccttga tctcgcacag ccccagcgtc
     1921 ccgtccgaac ccgtccccga cagcacgacg ccgatggcgc gccctttctg gtcttccgcc
     1981 agcgagcgga accagtagtc caccgggaga tgcggcccac gatccttcgg gcgcggggtg
     2041 aggatgagga tgccgccggc gatggccatg ttgacgttcg gcggaatgac g
//
LOCUS       DASVOD010000270         1583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_70772, whole genome shotgun sequence.
ACCESSION   DASVOD010000270 DASVOD010000000
VERSION     DASVOD010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70772"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VF720_05925"
     CDS             <1..1076
                     /locus_tag="VF720_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7878927.1"
                     /translation="IRLDDTQYRADVENSRASAKSAEANVQLSAARLDRARQNLERQK
                     KMKENKLVSDEAIEVAETEVKIAESELAARREEMAGARARIRSAEDALSKTIFTAPTG
                     GIISALNVKAGEIVITGTMNNPGTVILTIADQSKMQVEAEVDETDVIDIHPGQIAKIT
                     VDALPDTVFKGIVTTIGSSAQQSNTGSTEAATNFLVKVLFEADVPQLRPGMTADVEIT
                     TMTRTDVLAVPISSLVARDKKTIDRQKKRAEDMAAGGGKAIARDKKGGEDDMEDDSHK
                     APGDEKLLEGVFINDGGKAIFHTVKTGISDDTHIEVTGDLTLGSDIVTGPYKTLRELK
                     YGDPIKAAKKGGDGKKVMVKVGG"
     gene            1069..>1583
                     /locus_tag="VF720_05930"
     CDS             1069..>1583
                     /locus_tag="VF720_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009371845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7878928.1"
                     /translation="MADPGTNGALIDVRDLKKVYHLEGEDVRALDGVSLRIYRGEMVA
                     IMGPSGSGKSTLMNVIGCLDVPSSGTYILNGHEVSRMDDNELARIRNREIGFVFQTFN
                     LLPRADALHNVELPLIYSGMSSGERKKRAAQALDNVGLSDRKGHWPNQLSGGQRQRVA
                     IARALVNSPSL"
BASE COUNT          331 a          515 c          524 g          213 t
ORIGIN      
        1 tgatccggct cgacgacacc cagtaccggg ccgacgtgga gaattcgcgg gccagcgcca
       61 agagcgcgga agccaatgtg cagctctccg ccgcccgcct cgatcgggcc aggcagaatc
      121 tcgaacgcca gaagaagatg aaggagaaca agctcgtctc ggacgaggcc atcgaggtgg
      181 cggaaaccga ggtcaagatc gccgagtccg agctggcggc acgtcgtgag gaaatggcgg
      241 gcgcgcgcgc ccgcatccgc tcggccgagg acgccctctc gaagacgatc ttcaccgctc
      301 ccacgggcgg catcatctcg gcgctcaacg tcaaggcggg cgagatcgtg atcacgggga
      361 cgatgaacaa cccgggcacg gtcatcctca ccatcgccga ccagtccaag atgcaggtgg
      421 aggccgaggt cgacgagacc gacgtcatcg acatccaccc gggacagatc gcaaagatca
      481 cggtggacgc cctccccgac accgtcttca aggggatcgt gaccaccatc ggttccagcg
      541 cccagcagtc gaacaccggc tccaccgagg cggccaccaa cttcctggtg aaggtgctgt
      601 tcgaggccga tgtcccgcag ctccgaccgg gcatgacggc cgacgtggag atcaccacca
      661 tgacccgcac cgacgtgctg gccgtgccga tctcctcgct ggtggcgcgc gacaagaaga
      721 cgatcgaccg ccagaagaag cgggccgagg acatggcggc cggcggcggc aaggccattg
      781 ccagggacaa gaagggtggc gaggacgaca tggaagacga cagccacaag gccccggggg
      841 atgagaagct cctcgagggt gtcttcatca atgacggcgg caaggccatc ttccacacgg
      901 tcaagaccgg catctccgac gatacccaca tcgaggtgac gggagacctg accctcggct
      961 ccgacatcgt gacggggcct tacaagacgc tccgtgagct caagtacgga gacccgatca
     1021 aggccgccaa gaagggcggc gacggcaaga aggtgatggt gaaggtcggt ggctgatccg
     1081 ggcaccaacg gggcgctgat cgacgtccgg gacctcaaga aggtctatca cctggaaggg
     1141 gaggacgtgc gcgcgctgga cggcgtctcc ctcaggatct accgcggcga gatggtggcc
     1201 atcatggggc cgtccggctc gggaaagtcg accctgatga acgttatcgg ctgcctggac
     1261 gtgccctcct cgggcaccta catcctcaac ggccacgaag tgagccggat ggacgacaac
     1321 gagctggccc gcatccggaa ccgtgagatc ggcttcgtct tccagacctt caacctgctg
     1381 ccgcgggccg acgcgctcca caacgtggag cttccgctca tctattcggg gatgtcctcg
     1441 ggggagcgga agaagcgggc cgcgcaggcg ctcgacaacg tggggctttc cgaccgcaag
     1501 ggacactggc cgaaccagct ctccggcggc cagcgccagc gcgtggccat cgcccgggcg
     1561 ctggtgaaca gcccctcgct cat
//
LOCUS       DASVOD010000271         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_695805, whole genome shotgun sequence.
ACCESSION   DASVOD010000271 DASVOD010000000
VERSION     DASVOD010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695805"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VF720_05935"
     CDS             complement(<1..626)
                     /locus_tag="VF720_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7878929.1"
                     /translation="MMENKPASDAAFTGSVPALYDALLVPMLFEPYAADVVRRLSARP
                     PKRLLEVAAGTGAVTRAMAAGLPDTTAITATDLNPGMIERGRGVGTSRAVDWRVADAL
                     QLPFDDESFDAVVCQFGLMFFPDKPAGHREVRRVLVSGGIWLFSVWDRIEENEFPAVV
                     MDALARIFPDDPPRFLIRTPHGHFDREALARDLVAGGFTEAPHIDVVA"
     gene            complement(623..1492)
                     /locus_tag="VF720_05940"
     CDS             complement(623..1492)
                     /locus_tag="VF720_05940"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878930.1"
                     /translation="MAVYGVQMWVPAGDIIAEWLEANVPKERVVRWGGFGVNNGFYVK
                     VIFKEKRDAVAFHRHWYPEASDHDVPRWGASGGDTVDMSEFKPAEDGRRCAAEALALS
                     AYLPAGPRVAVIGSTSFWHAESEATCVELGRRLAALDGIVVLTGGVDGVGECVSRSFL
                     DALTEEGRDARVVHVLPHGSGAREFGRTLFAGSNMRDRREILGRVAEIYVAVEGGPGT
                     EHEAMVASRRGAPVVPLGRTGHALDLFGRREIPNFARPKLWARLVDERVSVATRAAAA
                     VEIVAAFLETPPR"
     gene            complement(1503..>1641)
                     /locus_tag="VF720_05945"
     CDS             complement(1503..>1641)
                     /locus_tag="VF720_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEX7878931.1"
                     /translation="YVNFLTDEETDRVAAAYGPNYGRLASIKKTYDPTNLFCTNMNIK
                     P"
BASE COUNT          299 a          583 c          503 g          256 t
ORIGIN      
        1 aacgccacca cgtcgatgtg cggggcctcc gtgaacccgc cggcgaccag atcacgggcg
       61 agcgcctcac ggtcgaagtg tccatgcggt gtgcggatca ggaatcgcgg cggatcatcg
      121 ggaaaaatgc gcgccagcgc gtccatgacg accgcgggga actcgttctc ctcgatccgg
      181 tcccacacac tgaacagcca gatgcctccg gaaaccagga cccgccgcac ttcacgatgc
      241 ccggccggct tgtcggggaa gaacatcagg ccgaactggc agacgacggc atcgaacgat
      301 tcgtcatcga acggaagttg cagcgcatcg gccacgcgcc agtcgaccgc ccgggacgtg
      361 cccacgcccc tcccgcgctc gatcatcccg gggttcaagt cggtggcggt gatggccgtc
      421 gtgtccggca gaccggccgc catggcacgc gtgaccgcgc cggtcccggc ggccacctcc
      481 agcaatcgct tcggtggtcg ggccgacaac cgacgcacga catcggccgc gtagggctcg
      541 aagagcatcg gcacgaggag cgcgtcgtac agagcgggga ccgagccggt gaaggcggcg
      601 tcgctcgccg gcttgttttc catcatcgcg gcggggtttc gagaaacgca gccacgattt
      661 ccacggcggc tgcggctcga gtcgcgacgg ataccctttc gtcgaccagg cgagcccata
      721 acttcggccg cgcgaaattg ggaatctccc gccggccgaa gaggtcgagg gcatggccgg
      781 ttcttcccag cggaacgacg ggtgcacctc gcctggaggc caccatggcc tcatgttcgg
      841 tgccaggccc cccttccacc gccacgtaga tttcggccac ccggccgaga atctcccgac
      901 gatcacgcat gttcgatccg gcgaagaggg tacggccgaa ctcccgcgcc ccgctgccgt
      961 gcggcagcac atgcaccacg cgggcatccc gcccttcctc ggtgagagca tcgaggaaac
     1021 ttcgcgagac gcactctccc acgccgtcca cgccacccgt aaggacgacg atcccgtcga
     1081 gcgccgccag ccgccggccg agctccacgc aggtggcctc gctttccgcg tgccagaacg
     1141 aggtgctgcc gatgactgca actcgcgggc cggccggaag ataagccgag agcgccaacg
     1201 cttccgccgc gcagcgacga ccatcctcag ctggcttaaa ttcgctcata tcgacggtgt
     1261 ctccgcccga ggcaccccac cgcggcacgt catggtcgga agcctcggga taccagtgtc
     1321 gatggaacgc gaccgcgtcc cgcttctcct tgaagatgac cttcacgtag aagccgttat
     1381 tgacgccgaa tccgccccag cgcacgacac gttccttcgg gacgttcgcc tcgagccatt
     1441 cggcgatgat gtcgccggca ggcacccaca tctggacacc gtagacagcc acaaactccg
     1501 ccctacggct tgatattcat gttcgtgcag aacaggttcg tcgggtcgta cgtcttcttg
     1561 atcgacgcca gccgaccgta gttcggcccg tacgcggccg ccacgcgatc ggtctcctcg
     1621 tccgtcagga agttgacgta c
//
LOCUS       DASVOD010000272         4916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_330754, whole genome shotgun sequence.
ACCESSION   DASVOD010000272 DASVOD010000000
VERSION     DASVOD010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330754"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..791
                     /locus_tag="VF720_05950"
     CDS             96..791
                     /locus_tag="VF720_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878932.1"
                     /translation="MSRRYHARLVALSFGIAALFRAAGSHAQETAPLTPLQPGAWALQ
                     FRVGNDFTLDSFKGGVLSVKHHSSARTALRLGLSTSLRFINQSDERLQGEVEASQEMD
                     ADEQSVALTLQRLKYLAPEKRLTPFWGVGPFVSWSHQNQEREIEPELLVEHGSASSWS
                     AGLTGNLGVEWCPTSHIGIHAEYGLSGGYSHQRQTTEVDETDTRPADSLTRESNSWFL
                     YSNSVLMGVSAYF"
     gene            complement(854..1336)
                     /locus_tag="VF720_05955"
     CDS             complement(854..1336)
                     /locus_tag="VF720_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878933.1"
                     /translation="MSPEPDREFLRHAVATLAYRGMKSVRGAPSGFGDFRAGSTSRMP
                     VEILSHIGDLLEWARFMAQGIRKWPDTVMGTWIEETVRFETVLAALDDYLAGDEPLGN
                     EARKIFQGPIADALTHVGQLNYLRRLAESPVRGENYFVADITTGRVGADQPAPNREFD
                     "
     gene            complement(1326..2204)
                     /locus_tag="VF720_05960"
     CDS             complement(1326..2204)
                     /locus_tag="VF720_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878934.1"
                     /translation="MAADPNTDIARTARRLILAATLVLLPVAAFAEEPNPTHLYDKFQ
                     FEVSGTAVGFNSELRVDANQGDDGTDIDAEDDLKAPEIKVQPRFELRWRPGRRHELEL
                     GYQFARRNSEHQLDNSFDFGDTTFMSGTHVRSHFDTDRAFLNYRYAFMAREKTQVGLT
                     FGVGALFIDTQVDALTEIQSEDGEREVEYGAGGSIIGPSAAIGPFANFQVGEDWYIHS
                     DLRWLKATVDRFTVRVLDAEAGTRWYPWTSVGFDLSGAVSSIALDVARPEDRDGLAGS
                     FEYSLLQLRLGLVYVP"
     gene            complement(2214..3416)
                     /locus_tag="VF720_05965"
     CDS             complement(2214..3416)
                     /locus_tag="VF720_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX7878935.1"
                     /translation="METVESQQEIPYKPGLEKVPAAISSVCYLDGDIGVLEYRGYLID
                     DLAHHVSYEETAYLLLNGELPTKAQLEIFEKSLKDHRSLQPAVIDVFKSLPVGGHPMH
                     ALEVATAATGMYFANIPFHAQSDPHVQQYHYEAAVRLIAKLPTMVAAWHRVRQGLDPI
                     APRTDLDHSANFLYMLDGHESDPELVKVFDACMVLHAEHEMNASTFATRVTASALSDP
                     YSVVVAAIATLRGPLHGGANERVLDMLAKIGAPENVETWVQSALASKETIWGIGHREY
                     RVKDPRAVILQEMNEIVFKKLGRTPLYDTAVKLEEYLATHPKFGAAPTLREQKFPNVD
                     FYSGIVYQKMGIPVDLYTPLFAMSRVAGWLAHYMEQVDAKKNKIFRPKQIYDGARERK
                     VTPIAQRA"
     gene            3730..4575
                     /locus_tag="VF720_05970"
     CDS             3730..4575
                     /locus_tag="VF720_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX7878936.1"
                     /translation="MVEGTEIGREYRIRRLESVIGRDEGVGLKFPDDRVSRQHARVMI
                     KRRPDATTDIVVQDLESRNGTWVNGKRIKGCVTLADGDKLQFGDTVLKFILQDDLDAK
                     FHEEVRYRVAYDQLTGLLNKESFDVALDSELRRCARHQLTLSVLMMDLDRFKSVNDSH
                     GHLMGSFVIREVGRLLKDNFRTTDVASRYGGEEFVAYLAESPLSEGSTVAQRIRHSIE
                     SHAFTRIMENGARLTIPVTISIGVSQFPTHGTTADQLVAAADQALYRAKEEGRNRVCV
                     AGTTG"
     gene            complement(4572..>4916)
                     /locus_tag="VF720_05975"
     CDS             complement(4572..>4916)
                     /locus_tag="VF720_05975"
                     /inference="COORDINATES: protein motif:HMM:NF027305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
                     /protein_id="HEX7878937.1"
                     /translation="MKKAEALKTYGEAARMQMIVERLPDLARAFAEPLASIDKVSILE
                     MGGGGNGHATGVNRFVGNVGGGMVAMSEFLKSNFDIDLAEMVKGRGAAEGQIGEAPGG
                     PAARTDKPPARG"
BASE COUNT          840 a         1604 c         1622 g          850 t
ORIGIN      
        1 ctcgtcgtgc cgcgcccctc cgacctcccc tgcatgtccg gacgaacgtg cccttcggtc
       61 cccgtccgac gttcgctacc agggagggaa gtgccatgtc acgccgttat cacgcccgcc
      121 tcgtcgccct gtcctttgga attgctgccc ttttccgcgc ggcagggtcc catgcccagg
      181 aaacggctcc gctgacaccg ctccagccgg gcgcctgggc gctccaattc cgggtgggaa
      241 acgacttcac cctggactct ttcaaggggg gcgtcctgtc ggtgaagcac cactcgtcag
      301 cccggacagc gctgcggctg ggcctttcga cgtcgctgcg attcatcaat cagagcgatg
      361 agcgtctgca gggggaggtc gaggcatccc aggagatgga cgccgatgag cagtccgtgg
      421 ccttgaccct gcaacggctc aagtatctgg cacccgagaa gcgccttacc ccattctggg
      481 gagttgggcc cttcgtcagc tggagccatc agaaccagga gcgagaaatc gaaccggagc
      541 ttctggttga gcacggatcg gcatcctcgt ggtcggccgg gctcactgga aatctcggcg
      601 tcgagtggtg tccgacatcg cacatcggca ttcatgccga gtacgggttg agcggggggt
      661 actcacacca gcggcaaacc accgaggtcg acgagacaga cacaaggcct gccgactcac
      721 tgacccgcga atcgaacagc tggtttcttt acagcaacag cgtcctgatg ggggtcagcg
      781 cctacttctg acgcgggact cgaccgaagc accatcgaaa caagcccggg cggcgcttcg
      841 gcgccgcccg tcctcaatca aattctcgat tcggcgccgg ctggtcggcg cccacgcgcc
      901 cggttgtgat gtcggccacg aagtagttct cgccgcgcac cggtgattcc gcgaggcgcc
      961 gcaagtagtt gagctgcccg acgtgcgtca aggcatcggc gatggggccc tggaagatct
     1021 tccgggcctc gttgccgagt ggttcatcgc cggcgaggta gtcgtcgagc gcggccagga
     1081 cggtttcgaa gcggacggtc tcctcgatcc acgttcccat gacggtgtcg ggccacttcc
     1141 ggattccctg ggccatgaaa cgcgcccatt cgagcaggtc gccgatgtgg gagaggattt
     1201 cgaccggcat ccgcgaggtc gagccggccc tgaagtcgcc gaagccggac ggcgcgccgc
     1261 gcaccgattt catgccgcgg taggccagcg tggctaccgc gtgacgcagg aactcgcggt
     1321 ccggctcagg ggacatagac gaggccgagg cggagctgca ggagggagta ttcgaaactc
     1381 cccgccagtc cgtcacgatc ctcggggcgc gccacgtcca gcgcgatgga gctgacggcg
     1441 cctgagaggt cgaatccaac cgacgtccag ggataccagc gggttccggc ctcggcgtcg
     1501 aggacgcgga ccgtgaaacg gtccaccgtg gccttgagcc agcgcaggtc ggagtggatg
     1561 taccagtcct ctccgacctg gaagttggcg aaggggccga tggcggcgct ggggccgatg
     1621 atcgagccgc cggctccgta ttccacctcg cgctctccat cctcggactg aatctcggtg
     1681 agggcatcca cctgggtgtc gatgaagagc gccccgacgc cgaacgtgag ccccacctgc
     1741 gtcttttccc tggccatgaa ggcatagcgg tagttcagga aggcccggtc ggtgtcgaaa
     1801 tggctccgga cgtgcgtgcc ggacatgaag gtcgtgtcac cgaagtcgaa tgagttgtcg
     1861 agctggtgtt cggagttgcg ccgggcgaac tggtagccga gctcgagctc gtggcgccgg
     1921 cccggccgcc accgcagttc gaagcggggc tgaaccttga tctcgggcgc cttcaggtcg
     1981 tcctcggcgt cgatgtcggt gccgtcgtcc ccttgattgg catccacccg gagttcggag
     2041 ttgaagccga cggcggtgcc ggagacctcg aactggaact tgtcgtacag gtgagtgggg
     2101 tttggctcct cggcgaaggc cgccacgggc aacaggacga gggttgccgc gagaatcaga
     2161 cgtcgcgcgg ttcgtgcgat atcggtgttc gggtctgcgg ccatcgtcac ctcctaggcg
     2221 cgttgcgcga tcggagtgac ttttcgctcg cgcgctccat cgtagatctg cttcgggcgg
     2281 aagatcttgt tcttctttgc atccacctgc tccatgtagt gggccagcca gccggccacg
     2341 cggctcatgg cgaagagcgg cgtgtagaga tccaccggga ttcccatctt ctggtagacg
     2401 atgccagagt agaagtcgac gttcgggaac ttctgttcac ggagcgtggg cgcggctccg
     2461 aacttggggt gcgtggcgag atactcctcg agcttgacgg ccgtatcgta cagcggcgtg
     2521 cgcccgagct tcttgaagac gatctcgttc atttcctgga ggatgacggc gcgcgggtcc
     2581 ttgacccggt actcgcggtg gccgatgccc cagatggttt ccttggaggc cagcgccgac
     2641 tgcacccacg tttccacgtt ttccggcgcg ccgatcttgg ccagcatgtc gaggacgcgc
     2701 tcattggcgc cgccgtgcag ggggccgcgc agcgttgcga tggccgccac caccacgctg
     2761 tacgggtcgg acagggccga ggccgtcaca cgggtggcga aggtggaggc gttcatctcg
     2821 tgctcggcgt ggagcaccat gcaggcgtcg aacaccttca ccagctcggg gtccgactcg
     2881 tggccgtcga gcatgtacag gaagttggcc gagtggtcga ggtcggtgcg cggcgcgatc
     2941 gggtcgagtc cctgccgcac gcgatgccag gcggccacca tcgtcggcag cttggcgatg
     3001 agccggacgg ccgcctcgta gtggtattgc tgcacgtgcg ggtcggactg cgcatggaac
     3061 gggatgttcg caaagtacat accggtggcg gccgtggcca cctccagcgc gtgcatcgga
     3121 tgcccgccca ccggcaacga cttgaacacg tcgatgacgg ccggctgcag cgaacgatgg
     3181 tccttgagcg acttctcgaa gatctcgagc tgcgccttcg tcggcagctc gccgttcagg
     3241 aggaggtagg ccgtctcctc gtagctgaca tgatgagcca ggtcatcaat caggtagccg
     3301 cggtactcga ggacgccgat atcgccgtcc aggtaacaga cgctggagat agccgccggc
     3361 accttctcca ggccgggctt gtacggaatt tcctgctggg actcgacggt ttccacgatg
     3421 acacctcggg cggagcaaag ggccctgacg ggccggaacg gctttaacag cggtaacgcg
     3481 gctcaagaga atagccccgg gaacggcgtc gatcaatggc cggccgggcc gccatggagg
     3541 cgggtcctaa agatccgtcc cgccgggccg atatcctgac gaggacccag agaggccggt
     3601 aacaccatgg agcacgcccg aaaaaagaaa cctgaggatg aacggcttcc acagccaccg
     3661 agcgatgcga cgcgcgtcgg gcccactgcc gtccgccccc cgactcggcg gcgccacgcc
     3721 agtctcatcg tggtggaggg gaccgaaatc gggcgtgagt accggatccg ccgcctcgag
     3781 tcggtcatcg gccgggacga gggagtcggg ctcaagttcc ccgacgaccg cgtgtcacgc
     3841 cagcacgccc gggtcatgat caagcgccgg ccggacgcca ccaccgacat cgtggtgcag
     3901 gacctggaga gccggaacgg cacctgggtg aacggcaagc ggatcaaggg gtgcgtgacg
     3961 ctggccgacg gcgacaagct ccagttcggc gacaccgtcc tcaagttcat cctgcaggac
     4021 gacctggacg cgaagttcca cgaggaagtg cgctatcggg tggcctacga ccagttgacc
     4081 ggcctgttga acaaggaatc atttgacgtg gccctcgact ccgaactccg gcgctgcgcc
     4141 cgccaccagc tcacgctctc tgtcctcatg atggacctcg accgcttcaa gtccgtcaac
     4201 gacagccacg gccacctgat ggggagcttc gtgatccgcg aagtcggtcg gctcctcaag
     4261 gacaatttcc gcaccaccga cgtggcctcc cgctacggag gcgaggagtt cgtcgcctac
     4321 ctcgccgaga gcccgctcag cgaggggagc accgtggccc agcgcatccg ccattccatc
     4381 gagagccacg ccttcacccg catcatggaa aacggtgcgc ggctgaccat tcccgtcacg
     4441 atctcgatcg gcgtctccca gttccccacc cacggcacga ccgccgacca gctggtcgcc
     4501 gccgccgacc aggcgctcta ccgcgccaag gaagaggggc ggaatcgggt atgcgtggcg
     4561 gggactacag gctagcccct ggccgggggc ttgtcggtcc gcgcggctgg tccccctggc
     4621 gcctcgccga tctgaccttc cgccgccccc cgccccttca ccatctccgc caggtcgatg
     4681 tcgaagttcg acttcaggaa ctccgacatg gccaccatgc cgccgcccac gttgccgacg
     4741 aagcggttca cgccggtggc gtggccgttg ccgccgcccc ccatctcgag gatcgacacc
     4801 ttgtcgatcg aggccagcgg ctcggcgaag gcccgggcga ggtcggggag gcgctccacg
     4861 atcatctgca tcctggcggc ttctccgtag gtcttgagcg cctcggcctt cttcat
//
LOCUS       DASVOD010000273         4052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_175790, whole genome shotgun sequence.
ACCESSION   DASVOD010000273 DASVOD010000000
VERSION     DASVOD010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175790"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF720_05980"
     CDS             complement(<1..218)
                     /locus_tag="VF720_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878938.1"
                     /translation="MRGRAGLRLAAAALVGFCGTGLDSHGAVTPDMRSATSEARTAPW
                     VSEADSSEWVGARLAEIDFGPNGRSEGG"
     gene            complement(215..2017)
                     /locus_tag="VF720_05985"
     CDS             complement(215..2017)
                     /locus_tag="VF720_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878939.1"
                     /translation="MTPLHAVALLFITPWAFAPPARAESGSLRPVVIQTITGAPGSVH
                     LPYVPDPADTLEDGRARLAMFDFGPDEQPAGRRIAFGTRSRNVAYGWEGGMVRQITRR
                     IAISGPYLCDFVEGEDSRFVIAVDPPDAVYQVQLTLGDPDSARGPIDILAGDEVVLDD
                     VSTTPGQPIEAVFESRAVAGRLSFRLRGEGCRAWAVVGAAVYGPKEARLTELYPISET
                     RSLVPPPDSLSRVSRSEAPQVLRRYIDFLLAWAPAEGGFSYAGAWYQNAYPLRTLLAG
                     SVLLDEPEYKEISFAVIDRFVATQRPDGRWLSNYFGKPGCQLSATNDTLSSNLADIGS
                     MTLAVGLAVPLADKERQDRWLAALTRYADNVVLPVQRPDGAFPNLRYEGRVFNHPYSV
                     ATATQAASLAVLGALTGEARYLEASEKAALWLANGIRTEGQVSFAPHDTSIVKIVQPS
                     SFGDVFYIVEALTWAGKYAPHREAQVTMRRALRNYFYGEGGLRAVSREGYWWSSRSLW
                     SDSKMGGIPYVLLRHEEGRASGDVAKMVANQLSWMADPGLSQRMGIQSPPASRNGEYG
                     LVATGFAGISVAAMIDPDVLVPVPRAPESDRGMR"
     gene            complement(2059..3741)
                     /locus_tag="VF720_05990"
     CDS             complement(2059..3741)
                     /locus_tag="VF720_05990"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX7878940.1"
                     /translation="MKGRTMPELWQATAAHASAPIGYPPPMRLIEWLDDRTGLPGGVR
                     AFLGKRLPRNLPWSYTLGSIALLLFVVQIVTGIFLAVYYAPTPDHAHDSLRWLESSIS
                     GGRLLRGMHHWGASFMVVAVLMHLLRVLVHGSYRKPREVNWLVGVKLLLFVMAFGLTG
                     YLLPWDQKAYWATQVTLNIARSVPLAGPFLAKVLGGGEHLGALTLTRFYAIHVLILPL
                     IAIGLVVWHVALVQRHGSSGPPSYEGNPKVDGDTFHPKQSARDAIAMTLVFAALVVTA
                     IVKPAPLEGLADPTDATYVPRPEWYFLGLFQLLKYFPGKLEVIGTVILPGIGTALLFA
                     LPWLDRSKSRRYGARKALLAGSLFAFAAAGALTLLGAMDKGGAPEPAWRRTGRTPFFA
                     AAAPSIAARRDCASCHRSDGFGAGPPLAGESWTRNATWIRAHLSDPAFVAPGTREAED
                     RLSTPDANMLMAWLEQLKDGEPAPSAAADTSAVSLILREGCIGCHSLDGAGGRNGPSL
                     DGVAKRHNADWIVNYLPDPEAINPEAKMRAYPDLTLDERTSIASYLIRHTED"
     gene            3728..>4052
                     /locus_tag="VF720_05995"
     CDS             3728..>4052
                     /locus_tag="VF720_05995"
                     /inference="COORDINATES: protein motif:HMM:NF025223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX7878941.1"
                     /translation="MRPFMFLLLVLRLLVTAGVSAADPVVPPPADPAVDGVWNHPELT
                     SYPTPRHRYALVYDSIRHRALLYGGLNAEGNRTFDDVWALDLDEPKGWARVPVRSSDD
                     EGVGRL"
BASE COUNT          667 a         1364 c         1404 g          617 t
ORIGIN      
        1 ctgccccctt ccgaccggcc gttggggccg aagtcgatct cggcgagtcg ggcgccgacc
       61 cattcggagg aatcggcctc ggacacccat ggtgccgtgc gggcttccga ggttgcgctc
      121 cgcatgtcgg gtgtcacggc gccgtgcgaa tcgagccccg tgccgcaaaa gcccacgagt
      181 gccgcggcgg ccaaccggag ccccgctcgc cccctcatcg catgccccgg tcgctttcgg
      241 gtgcccgcgg caccggcacc aggacatcgg gatcgatcat ggccgccacg ctgatgccgg
      301 cgaagccggt ggccacgagg ccgtactcgc cgttcctgct ggccggcggc gactggattc
      361 ccatccgctg cgagagcccc ggatcggcca tccacgacag ttggttggcc accatcttcg
      421 ccacgtcgcc gctcgcgcgt ccttcctcgt ggcggaggag cacgtagggg atgcccccca
      481 tcttggaatc gctccagagg ctgcgggacg accaccagta cccctcgcgc gaaacggccc
      541 tgaggccgcc ttcgccgtag aagtagttgc ggagcgcgcg ccgcatcgtc acctgcgcct
      601 cgcggtgcgg ggcgtacttg ccggcccagg tgagggcctc gacgatgtag aagacgtctc
      661 cgaacgagct gggctggacg atcttgacga tgctggtgtc gtgaggcgcg aacgatacct
      721 gtccttcggt gcggatcccg ttggcgagcc agagcgccgc cttttcggag gcctcgaggt
      781 agcgcgcctc accggtcaat gccccgagca ccgccaggct ggcggcctgc gtggcggtgg
      841 ccacggagta ggggtgattg aagacgcgcc cttcgtaccg gagattcgga aacgctccgt
      901 ccggacgctg caccggcagg accacgttgt cggcgtacct ggtgagcgcg gccagccagc
      961 gatcctgccg ctccttgtcg gccagcggaa cggccagccc cacggccagc gtcatcgagc
     1021 cgatgtcggc caggttgctc gacaacgtgt cgttggtggc cgagagctgg caccccggtt
     1081 tgccgaaata attcgagagc cagcgtccgt cggggcgctg cgtggccacg aaacgatcga
     1141 tcaccgcgaa cgagatctcc ttgtactccg gttcgtcgag gagcacgctc ccggcgagca
     1201 gcgtgcgcag cgggtaggcg ttctggtacc aggcgccggc gtaggagaac cctccttccg
     1261 ccggggccca ggccaggagg aagtcgatgt acctccggag gacctgtggc gcctccgacc
     1321 gtgacacgcg gctcaacgag tcggggggcg gcaccaggct gcgggtttcc gaaatcgggt
     1381 agagctcggt gagacgcgcc tccttcgggc cgtacacggc ggcgcccacc acggcccagg
     1441 cccggcaccc ctctccccgg aggcggaacg agaggcggcc ggctactgcc cgggattcga
     1501 agacggcctc gatcggctgg cccggggtgg tggaaacgtc gtcgaggacg acttcgtcac
     1561 cggcgagaat gtcgatcggc ccgcgggccg agtcgggatc gcccagcgtg agctgcacct
     1621 ggtacacggc atccggcggg tcgacggcga tgacgaagcg ggaatcctcc ccctcgacga
     1681 agtcgcacag gtacgggccg gagatggcga tccgccgcgt gatctggcgg accatgcctc
     1741 cttcccatcc gtaggccacg ttccggctgc gcgtaccgaa ggcaatgcgt cggccggccg
     1801 gctgttcatc gggcccgaag tcgaacatcg cgagtcgcgc gcggccatcc tcgagcgtgt
     1861 cagccggatc ggggacgtac ggaagatgca ccgaccccgg ggcgccggtg atggtctgga
     1921 tgacgacggg acggagcgat cccgattccg cccgggcggg gggagcgaag gcccaggggg
     1981 tgatgaacag gagcgcgacc gcatgcagag gagtcatgcg catcgtgaaa gagtgtagcc
     2041 gaatctcttt cgtgatggtc agtcttcggt gtgtcgaatg agataggagg cgattgacgt
     2101 tcgttcgtcg agggtcaggt cagggtaggc gcgcatcttc gcctcgggat tgatggcctc
     2161 cgggtcggga aggtagttca cgatccagtc ggcattgtgg cgttttgcca caccatcgag
     2221 gctcggaccg ttccgaccgc ccgcgccatc caggctgtgg caaccgatgc atccctcgcg
     2281 aaggatgagc gacacggccg acgtgtcggc cgcggcggat ggcgcaggct cgccgtcctt
     2341 cagttgctcc agccacgcca tcagcatgtt ggcgtcgggc gtcgacaacc gatcctcggc
     2401 ctcgcgcgtt ccgggagcca cgaacgccgg atcggacaga tgcgcgcgga tccacgtggc
     2461 gtttcgcgtc cacgactcgc cggcgagggg aggcccggcg ccgaacccgt cactccgatg
     2521 gcacgacgcg cagtcgcgcc gcgccgcgat ggagggcgcc gccgcggcga agaacggtgt
     2581 gcgtcctgtc cgcctccagg ccggctcggg cgcgccaccc ttgtccatgg cgcccagcag
     2641 cgtgagcgcc ccggccgccg cgaaggcaaa gaggctgccg gccaggagcg ccttgcgcgc
     2701 gccgtagcgc cggctcttgc ttcggtcgag ccacggcaag gcaaagagaa gcgcggtgcc
     2761 gatccccggc aggatcacgg tgccgatcac ttccagcttg ccgggaaaat atttcaacag
     2821 ctggaagagt ccgaggaaat accactccgg acggggaacg tacgtggcgt cggtcgggtc
     2881 ggccagcccc tcgagcggcg ccggcttcac gatggccgtc accaccagcg cggcgaacac
     2941 caacgtcatg gcgatggcgt cccgcgccga ctgcttcggg tggaacgtgt cgccatccac
     3001 cttcggattc ccttcgtacg acggcggccc ggacgaaccg tgccgctgca ccagcgccac
     3061 gtgccacacc acgaggccga tcgcgatcaa cggcagaatg aggacgtgga tggcgtagaa
     3121 gcgcgtcagc gtgagtgcgc cgaggtgctc gccacctccg agcaccttgg ccaggaacgg
     3181 cccggccagc ggcaccgacc tggcgatgtt cagcgtcacc tgcgtggccc agtaggcctt
     3241 ctggtcccac ggcaggagat aaccggtaag gccgaacgcc atcacgaaga ggaggagttt
     3301 cacaccgacg agccagttga cctcgcgcgg cttccggtac gaaccgtgga cgagcacgcg
     3361 caacaggtgc atcagcaccg ccaccaccat gaagctggcg ccccagtggt gcatgccgcg
     3421 caggagccgg ccaccggaga tcgatgactc gagccaccgc aggctgtcgt gcgcatggtc
     3481 gggcgtgggg gcgtaataca cggccaggaa aatgccggtg acgatctgca cgacgaagag
     3541 gaggagggcg atcgagccga gcgtgtagct ccagggaagg ttccgcggca gcctctttcc
     3601 gaggaacgcg cgcacgccac ccggaagccc ggtgcggtca tccagccact cgatcagccg
     3661 catcggcgga ggatacccga tcggggcgct ggcgtgagcg gcggtggcct gccacaattc
     3721 cggcatcgtg cgccctttca tgtttctcct gctggtcctc cgactcctcg ttaccgccgg
     3781 cgtgtcggcc gccgatccgg tcgtcccgcc ccccgccgat ccggccgtcg atggcgtgtg
     3841 gaaccacccg gagctcactt cctatccgac gccgcgccat cgctacgcgc tcgtctacga
     3901 cagcattcgt caccgggcgc tcctgtacgg cggactgaac gcggaaggaa accgcacctt
     3961 cgacgatgtc tgggcgctcg acctggatga gccgaagggt tgggcaaggg tgccggtgcg
     4021 ctcgtcggat gacgaaggcg tcgggcgcct gg
//
LOCUS       DASVOD010000274         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_592511, whole genome shotgun sequence.
ACCESSION   DASVOD010000274 DASVOD010000000
VERSION     DASVOD010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592511"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /gene="dnaB"
                     /locus_tag="VF720_06000"
     CDS             complement(<1..1132)
                     /gene="dnaB"
                     /locus_tag="VF720_06000"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX7878942.1"
                     /translation="MINLPESGGADSGGRVPPQALDAERMVLGAMMLDREAIGRGIEV
                     LDEDAFYRRSHKRIYQALISLYDKGEAVDVITVSNELDTRGWIEEVGGLSYLSGLIAE
                     VGTAAHVGYHAKIVLEKATLRRLITAAGSIMTDAYEQRLPSSEVLDKAEESIFAIADQ
                     RDKKAFTSLREMMKPTFEGIEEQARRKNPITGVETGFADLDTCTSGFQPSDLIIVAGR
                     PSMGKTAFCLNVAENAALKFGRPVAIFSIEMSKEQLIRRLLSSQSRVSGQRLRTGFLR
                     DSDWPALAAAANRLSAAPIYIDDSPSPTVLEMRAKARRLKSEVDLGMIIIDYLQLVRG
                     SGDVENRQQEISQISRSLKALAKELKVPVVALSQLSRAVESRG"
     gene            complement(1299..2255)
                     /locus_tag="VF720_06005"
     CDS             complement(1299..2255)
                     /locus_tag="VF720_06005"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7878943.1"
                     /translation="MSDDRAELAKLARDVRRQVDREARAGRGTVVDPALAEWMATKPP
                     AAQGPRLMASGRVTNPKAAGLPSMPIRSGGMESLENIITPAAAPTPRAPLPKPPVIGA
                     PSAPAKTSTALATVPLPADDPRDIPAMTEALAVARAEALACVKCGLCEGRTQVVYSGG
                     FPRHPLMFVGEAPGFHEDQQGVPFVGRAGQLLTDIITAMGFDRNDVYIANVLKCRPPE
                     NRDPLPHEIEACWPWLEQQIDLVKPRVICTVGKYSAQLLTGAGPKATIGTLRGKVFRF
                     KGIPVVPTYHPAFLLRSPSFKRAAWEDVQLVREIYLAGSDGN"
     gene            complement(2252..>3172)
                     /gene="coaBC"
                     /locus_tag="VF720_06010"
     CDS             complement(2252..>3172)
                     /gene="coaBC"
                     /locus_tag="VF720_06010"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX7878944.1"
                     /translation="AHGLGDDALTTVLLAAGPRVHFAPAMNHRMWNHPATQANVATLM
                     SRGAVIIEPGAGYQACGETGVGRMAEPAEIHAAIRTALATSRDLVGRTIVVTAGRTEE
                     PVDPVRVLTNRSSGRMGVALAEEARDRGANVVLVSGVLSVDPPAGITVMRAPTAESMR
                     EAVATAMPHADAIIAAAAVADFRPAAPANEKIKRAGGMDAIALEPTTDILKEAAATRR
                     PGQYFVGFALETNGGLDAARKKLVEKKVDLIVYNNPTNHGSGFGGDTNEVTLVDAGGE
                     ESLPLQSKRAVARAIVDRVARGMNGAKPSR"
BASE COUNT          472 a         1043 c         1116 g          541 t
ORIGIN      
        1 cgccgcgtga ttcgaccgca cgcgagagct gcgagagggc cacgaccggc accttgagtt
       61 ccttggccag cgccttgagc gagcggctga tctggctgat ttcctgctgg cggttctcga
      121 catcgccgga gccgcggacg agctggaggt agtcgatgat gatcatgccg aggtcgactt
      181 ccgacttgag gcggcgggcc ttggcgcgca tctcgaggac ggtgggcgac ggcgagtcgt
      241 cgatgtagat gggggcggcc gagagacggt ttgcggcggc ggcgagggcg ggccagtcgg
      301 aatcgcgcag gaagccggtg cgcaggcgct ggcccgacac gcggctctgg ctggacagga
      361 ggcggcggat gagctgctcc ttcgacattt cgatgctgaa gatggccacc gggcgtccga
      421 acttgagggc ggcgttctcg gccacgttga ggcagaaggc ggtctttccc atcgagggac
      481 ggccggccac gatgatgagg tcggacggct ggaagccgct ggtgcaggtg tcgaggtcgg
      541 cgaagcccgt ctcgacgccg gtgatcgggt tcttgcgccg ggcctgctcc tcgatcccct
      601 cgaaggtggg cttcatcatc tcgcgcaggc tggtgaaggc cttcttgtcg cgctggtcgg
      661 cgatggcgaa gatcgattcc tcggccttgt cgagcacctc gctcgagggg aggcgctgct
      721 cgtaggcatc ggtcatgatc gagccggccg cggtgatgag gcggcgcagc gtggccttct
      781 cgaggacgat cttggcgtga taaccgacat gcgccgcggt gccgacctcg gcgatgaggc
      841 ccgacaggta cgagagcccc cccacttcct cgatccagcc tcgcgtgtcg agttcattgg
      901 acacggtgat cacgtcaacc gcctcgccct tgtcgtagag gctgatcagc gcctggtaga
      961 tacgcttgtg cgaacggcgg tagaacgcat cctcgtcgag cacctcgatg ccgcggccga
     1021 tggcctcgcg gtcgagcatc atcgcaccga gcaccatgcg ctcggcgtcg agggcctgtg
     1081 gcggtacgcg gccgccggag tcggcgccgc ccgattcggg aaggttgatc acgaacatct
     1141 cccggacacg ttcgaggagt gcgaaggatc gatcacgaca gtcattgcag tgagtgaaag
     1201 gctaccttgt gcgacaggcc ggctcaagta gcgacggcgt ttcggtgcaa caatgcgatg
     1261 tgcacagttg tggaaactgt ggacaagctg attccgattc agttcccgtc gcttcccgcc
     1321 aggtagattt cgcgcacgag ctgcacgtct tcccacgccg cccgcttgaa cgacgggctc
     1381 cgcaggagga acgccgggtg atacgtgggc accaccggga tccccttgaa gcggaagacc
     1441 tttccgcgca gcgtgccgat ggtggccttc gggccggcgc cggtcaggag ttgcgccgaa
     1501 tacttcccca ccgtgcagat gacgcgcggc ttcaccaggt cgatctgctg ctcgagccac
     1561 ggccagcacg cctcgatctc gtgcggcagc ggatcacgat tctccggagg ccggcacttg
     1621 agcacgttgg cgatgtacac atcgttccgg tcaaacccca tggccgtgat gatgtcggtg
     1681 aggagctgcc cggcgcggcc gacgaacggc acgccctgct ggtcttcgtg gaagccgggc
     1741 gcctccccca cgaacatgag cggatggcgc gggaagccgc cggagtacac gacctgcgtt
     1801 cgtccttcgc agagtccgca cttcacgcag gccagcgcct cggcgcgggc caccgccagg
     1861 gcttccgtca tggccggaat gtcgcgcggg tcgtccgccg gaagcggaac ggtggcgagc
     1921 gctgtcgaag tcttcgcggg agcggacggc gcgccgatga ccggaggttt cggcagtggc
     1981 gcccgtggtg tcggcgcagc ggccggagtg atgatgttct cgagcgactc cattcccccg
     2041 gaacgaatcg gcatcgacgg gagccccgca gccttcggat tcgtcacgcg acccgacgcc
     2101 atgagacgcg gtccctgcgc ggccggtggc ttcgtggcca tccattcggc cagtgccgga
     2161 tcgacaaccg tgccgcggcc ggcacgggcc tcgcgatcca cctggcggcg gacgtcgcgc
     2221 gccagcttcg cgagttccgc gcggtcgtcg ctcaccggga cggcttcgcg ccgttcatgc
     2281 cacgcgccac acggtcgacg atggcgcggg ccaccgcccg cttcgactgg agaggcagtg
     2341 actcttcgcc gcccgcgtcg acgagcgtca cttcattggt atcgccgccg aatccggagc
     2401 catggttcgt gggattgttg tagacgatga ggtccacctt cttctcgacg agcttcttcc
     2461 gcgccgcgtc gagcccgccg ttcgtttcca gcgcgaagcc gacgaagtac tggccggggc
     2521 ggcgggtggc ggcggcttcc ttgaggatgt cggtggtcgg ttcgagggcg atggcgtcca
     2581 tccccccggc gcgcttgatc ttttcgttcg ccggggcggc cggccggaag tcggccacgg
     2641 cggcggcggc gatgatggca tccgcgtgcg gcatggccgt ggccaccgcc tcgcgcatcg
     2701 attccgccgt cggcgccctc atgacggtga tccccgccgg cggatcaacc gacagcacgc
     2761 ccgacaccag caccacgttg gccccgcgat cgcgcgcttc ctcggccagg gccactccca
     2821 ttcgcccgct gctccggttc gtgagcacgc ggaccgggtc gaccggctct tcggtgcgcc
     2881 cggccgtgac gacgatcgtc cggccgacca ggtcgcgcga cgtggccagc gcggtacgga
     2941 tggcggcatg gatctcggcc ggctcggcca tgcgccccac gcccgtttcg ccgcacgcct
     3001 ggtaacccgc gcccggctcg atgatgaccg cgccacgtga catgagcgtg gccacgttcg
     3061 cctgcgtggc cggatggttc cacatgcgat ggttcatggc cggtgcgaag tgaacgcgcg
     3121 gtcccgcagc cagcaacacg gtggtgagcg cgtcgtcgcc caggccgtgg gc
//
LOCUS       DASVOD010000275         3908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_748840, whole genome shotgun sequence.
ACCESSION   DASVOD010000275 DASVOD010000000
VERSION     DASVOD010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748840"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..2826)
                     /locus_tag="VF720_06015"
     CDS             complement(55..2826)
                     /locus_tag="VF720_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878945.1"
                     /translation="MIGRAARARTLFPAALALLVTMACLVAPLALLLTPTPAAAQAIS
                     FGKNKIRYNNFEWRVRESDHFDLYYYPEEESLAVIALAQAEESYVELSARWGFAVERR
                     IPFIIYASHAAFEQTNLTPGFLPEGVAGFTEFVKGRVVVPFNGSVSDFRGTIHHEMVH
                     VYQLAKGESVFGRHYRGRPVDAPLWMTEGQADLWAGDWDGIGEMVLRDLVLSNQLPSI
                     PDLWQFYGTFTIYKVGQDLCGYLEREFGRDIIPRLYENLWKAESFSQALTMTIGVNET
                     DLSERWHRDLKLRYYPDIDDARSPTLDARPLAAGGGANFKPCVLPNGDGLPPNKFLYM
                     SPRTGYTNIYMASLNAPDQETEIIITGERNAQYEALHPFRSRIDVTPAGRLAFISKYH
                     DRDALYFYDIKERKELGRYQFEGLVGLLSPTQSKDGRVVAFSGLGLDGRSDLYLFYVE
                     DNRLERITKDWYQDLDPAISPDGRMVAFSSDRTPTGHEGDRTLFLYDRLTGKIRALTR
                     GRQVDSTPDWSNSGDRIAFSSDRHGTPQIHVIDLDGPTIRVTAMQGGAFDPVWLENDT
                     GMLFTVYHRGRYGIYRLNDIEPLDSPVTGSSPLAADLGPTLPPADTLGVEPWRLEQGL
                     ALPPSREASYQRRYTFDLAQAGVALDPSLGYGEGLQASLSDQLSDRLIFFQLSNSASS
                     TSELLSRLNVSGNYFNVSRRFRTGYGAYHVAGDFRDERDVLYFERRAGVDLIGSYAFS
                     KFDRIDASLFVFHSDRTADSFRPAREAVLASNYVSLVHDNALWIETGPIAGSRWNLTA
                     GVTTDLERASIENTALLVDWRSYLRTGLRSAYAVRFQGRISEGTGAQRFTLGGPYSLR
                     GYPRRAFYGTRSFLLNQEIRFPILEGAVLRFPFGGVGLPGVQAALFGDLGQAWEEGEG
                     FIPDPLGSF"
     gene            3414..3782
                     /locus_tag="VF720_06020"
     CDS             3414..3782
                     /locus_tag="VF720_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEX7878946.1"
                     /translation="MDFDDRTITCRDCGTQFVFTAGEQAFYQQKGFSNEPTRCPSCRR
                     AKKEMGGGGGGGGGGFRSSGGDSYGGGRGGGGGGGGNRQLYKAVCSSCGVETQVPFPP
                     SPDRPVYCRNCYNSRRGGGY"
BASE COUNT          702 a         1262 c         1294 g          650 t
ORIGIN      
        1 cacgaagtcg agacgcagga tgaggagccc gccgaaactc gagcgcaggc cgattcagaa
       61 actgccgagc ggatcgggga tgaacccctc gccttcctcc cacgcctgcc cgagatcgcc
      121 gaacagcgcg gcctgcacgc cggggaggcc gacgccgccg aacgggaagc ggagcaccgc
      181 cccctcaaga atcgggaagc ggatttcctg gttgaggaga aaggaccgcg tgccgtagaa
      241 cgcgcgccgc ggatagccgc gcagcgaata cgggccgccg agcgtgaaac gttgcgcacc
      301 cgttccctcg gagatgcgcc cctggaaccg gaccgcgtac gccgatcgaa gaccggtgcg
      361 caggtagctc cgccagtcga ccaggagggc cgtgttttcg atgctggccc gctcgaggtc
      421 ggtcgtgacg ccggccgtca ggttccagcg tgagccggcg atcggaccgg tctcgatcca
      481 gagcgcgttg tcgtgcacca gcgacacgta gttgctggcc aggaccgcct cgcgcgcggg
      541 tcggaacgaa tcggccgtcc ggtcggaatg aaagacaaag agcgaggcgt cgatgcgatc
      601 gaacttggag aaggcgtaac tgccgatcag gtcgacgccg gcccgccgct cgaagtagag
      661 gacatcccgt tcgtcgcgga aatcgcccgc cacgtggtag gcgccgtagc cggtccggaa
      721 acgccgcgag acgttgaagt agttgcccga cacgttgagc cgcgacaaca gctcggaggt
      781 actcgacgcc gagttggaga gctgaaagaa gatcagtcga tccgacagct gatcggagag
      841 cgaggcctgc aacccttccc cgtaaccgag gctcgggtcg agcgccacgc cggcctgcgc
      901 caggtcaaac gtgtagcgcc gctggtacga cgcctcgcgg ctcggcggca aggcaagtcc
      961 ctgctcgagg cgccacggct ccacgcccag cgtgtcggcc ggcgggagcg tcgggccgag
     1021 gtcggccgcc agcgggctcg agccggttac cggcgaatcg agcggctcga tgtcgttgag
     1081 ccggtaaatg ccgtaacgcc cccggtgata cacggtgaag agcatcccgg tgtcgttctc
     1141 gagccacacc ggatcgaacg cgcctccctg catggcggtc acccggatgg tgggcccgtc
     1201 caggtcgatg acgtggatct gcggcgtccc gtgccggtcg ctggaaaagg cgatccggtc
     1261 gccgctgttc gaccagtcgg gcgtgctgtc gacctggcgc ccgcgcgtga gcgcgcggat
     1321 cttgccggtc aggcggtcat agagaaacag ggtgcggtcg ccctcgtggc cggtcggcgt
     1381 gcggtccgac gagaacgcca ccatccggcc gtccgggctg attgcggggt ccaggtcctg
     1441 gtaccagtcc ttggtgatcc gctcgagacg gttgtcctcg acgtaaaaga gatacaggtc
     1501 cgagcggccg tcgagaccga gcccggagaa cgccaccaca cgcccgtcct tgctctgcgt
     1561 cggcgagagg agtcccacca atccctcgaa ctggtaccga cccagttcct tccgctcctt
     1621 gatgtcatag aaatacaggg catcacggtc gtgatacttg gagatgaacg ccaggcgccc
     1681 cgccggcgtc acgtcgatcc ggctgcggaa cggatggagc gcctcgtact gcgcgttccg
     1741 ctcccccgtg atgatgatct cggtctcctg gtccggcgcg ttgagcgacg ccatgtagat
     1801 gttggtgtag ccggtgcggg gagacatgta gaggaacttg ttggggggca gcccgtcgcc
     1861 gttcggaagg acacacggct tgaagttggc gccgcctccc gcagccagcg gccgcgcgtc
     1921 gagcgtcggc gagcgggcgt catcgatgtc ggggtaataa cggagtttca ggtcccggtg
     1981 ccaccgttcg gagaggtcgg tctcgttcac cccgatggtc atcgtcagcg cctggctgaa
     2041 cgactccgcc ttccacaggt tttcatacag tcgggggatg atgtcgcggc cgaattcccg
     2101 ttccaggtag ccgcacaggt cctgccccac cttgtagatg gtgaacgtcc cgtagaattg
     2161 ccacaggtcg ggaatcgagg gcaactggtt cgagaggacg agatcgcgca gcaccatttc
     2221 gccgatgccg tcccagtcgc cggcccagag atcggcctgc ccttccgtca tccagagcgg
     2281 cgcgtcgacc ggccggccgc ggtagtgccg cccgaagacg ctctctccct tggcgagctg
     2341 gtacacgtgc accatctcgt ggtggatcgt cccccggaag tcggaaaccg acccgttgaa
     2401 cggcaccacc acgcgcccct tcacgaactc ggtgaagccg gccacgcctt cggggaggaa
     2461 gccgggcgtg aggttcgtct gctcgaaggc cgcatggctg gcgtagatga tgaacgggat
     2521 ccgccgttcc accgcaaagc cccaccgcgc cgagagctcc acgtacgact cttcggcctg
     2581 cgccagcgcg atcaccgcca gcgactcctc ctccgggtag taatagaggt cgaagtgatc
     2641 gctctcccgc acccgccatt cgaaattgtt gtagcggatc ttgttcttcc cgaagctgat
     2701 ggcctgggcg gcggcgggtg tgggggtgag gaggagggca agcggggcga cgaggcaggc
     2761 catcgtgaca aggagggcca gggcggcagg aaagagggtc cgggcgcgag cggcccgccc
     2821 aatcatgcct ggcccgggcg cagctcccgg gcgcccgtcc ccaggtcccg aaacaggccg
     2881 cacgaagacc gcgggcccgc tccatgcccg tttagcgcgg tctggcccga cgcgggaaag
     2941 cgcttcggcc agaaggttca tccaagttgc accacgcatt gatcttattg tcggatttcc
     3001 ggggagatga cacaaccgga gacttggggg aaagcccgaa ccggctcaaa aattacttga
     3061 ttgttggcgg ggggaggagt ttgatctacg taacggatca gcgccaaccg ccttcctcga
     3121 gtctcccgac agacccttgg gaccggctgt ccaccatcaa cgccaatatc ccgagaaaac
     3181 gtgtctgtcg aaatcctgct tcatcgaacc gcaagaatac ctctccggaa attcctaccg
     3241 ttttccgcgt ccgcacgccc ggctccggga tccctctgcc ccgatcgcgc ccgtgacgtg
     3301 tcgccgaatg ctcccccccc cggaggaacc tgcatgacgt caacgtgacc gcctgcgaac
     3361 cccgtcacaa ctcatcagtt tccgatccca gtcatttccc cgagaggtag ttcatggatt
     3421 tcgatgatcg caccatcacc tgccgtgatt gtggcacgca attcgtgttc accgcaggtg
     3481 agcaggcttt ctaccagcag aaggggttct cgaacgagcc cacccgttgc cccagctgtc
     3541 gccgcgccaa gaaggagatg ggcggcggag gcggtggcgg cgggggtgga ttccgctcca
     3601 gcggtggcga ctcgtacggt ggaggtcgtg ggggtggcgg tggtggcgga ggcaaccgcc
     3661 agctctacaa ggcggtctgt tcgtcgtgcg gcgtggagac gcaggttccc ttcccgccct
     3721 cgccggatcg accggtctac tgccgcaatt gttacaactc gcgacgcggc ggagggtact
     3781 gagttccgcc acgtgatgcg cggcctgtag ccattacagg atcgtcaaat ggtcggggcc
     3841 cggaggtcat ccctccgggc cccgattttt tcaaagcgcc aagccgaccg taagccgaat
     3901 cctgtccg
//
LOCUS       DASVOD010000276         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_748851, whole genome shotgun sequence.
ACCESSION   DASVOD010000276 DASVOD010000000
VERSION     DASVOD010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748851"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VF720_06025"
     CDS             complement(<1..1469)
                     /locus_tag="VF720_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878947.1"
                     /translation="MHRALATVIAHSRRTRPLLLLVAAFGLAVLMAPSVDGKGQRIVR
                     RPELVGPFDGEVVTEAGLRFSFVPAPGTTRQFLLVSREPFSTDGLDKLPEGDGIRVFP
                     VTTPVVDGVSLGLTALESGWYWAVASDDGDGGSFAVSPVRRVEVRPSWQVSDGGSPLI
                     RSESRGRLAPTMTRQRDRNLHLAAGYDFDPTNGEPAFTAAQKSGVTAENDERVGVIVQ
                     FDGPTTPDDLAQLQAAGAVPVTPLAGDAWLVRIFPESRSALARGARVTWTGDWHPAYR
                     LAPALKAKAEGNAAAAAVPVEMTVLLFNDAPVGRDRQRLVNLGAEVTAESDNGINRLL
                     RVTMPAARALEAARLPGVQWVEPWITPTFENVNAQWVVQTNVNSNRKIWDQGIDGTGQ
                     VVHNSDSGITTAHNQFRDAGVPITTFGDYPTHRKIIAYHLGSNNPAITFGDHSGASFH
                     GTHTNGTVAGDDSPVGGADLRDGMAKGARIFFSDISGTTL"
     gene            complement(1612..2661)
                     /locus_tag="VF720_06030"
     CDS             complement(1612..2661)
                     /locus_tag="VF720_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878948.1"
                     /translation="MKRFAVPALFLGLCLAGILVARIEPLFLATEMSIRFGRNSLLVL
                     ALIIPILAGLGLNFGITIGAMAGQVAAILVTHWGVKGLGGFGLAALIAMPLAILFGWL
                     VGWLFNMAKGREMITGLIAGYFANGIYQFVWLFLAGTLIPFKNEAMILPQGYGLRNTV
                     DLTGLQYALDDLIPLKLAIFGGNVRVPVATILVVVVFCFLIVAFLRTKLGQQLKAMGQ
                     DAHVAAIAGIPVDRNRIIATILSTVLGAWGQLLFLQNLGTMNTYNSHEQVGMFAIAAL
                     LVSGATVKKATVAQALVGTLLFHTLFVVSPLAGKALAGDAQIGEYFRVFVAYAVITLA
                     LVLHAWQGRRKPKAA"
     gene            complement(2658..3734)
                     /locus_tag="VF720_06035"
     CDS             complement(2658..3734)
                     /locus_tag="VF720_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7878949.1"
                     /translation="MSAPKETLSRSTAGDRWREFVREAGVPRLIILGFLVLLFLIAAV
                     TGMNLASLLSQSIDRVARNGILVLALLPAIQGGLGLNFGLPLGILCGLVGCVSVINFG
                     AAGWPGFFLATLVGSLLAVPIGGIYGWLLNRTRGQEMMVGTYVGFAMVSLMSIFWLLA
                     PFHNPKLVWAIGGKGLRTTLTLNDTFEKILDKFLQVRIGDFVLPTGTLLYFGFFCLLV
                     WLFFRTRAGLSISAAGDNPRFARSSGIQDANTRVHASILTTVLGAVGIVAFSQSYGFV
                     QLYQAPLMMAFPAVACILIGGASVMRASIRNVVVGTFLFQTILTIALPVTSKVVVGDV
                     SETARLVIQNGMILYALTRRGDKG"
     gene            complement(3731..>4415)
                     /locus_tag="VF720_06040"
     CDS             complement(3731..>4415)
                     /locus_tag="VF720_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7878950.1"
                     /translation="GEILGLGGLAGYGKVGVANGIMGLHPARGAITVRGRPLKLNDPG
                     ASLGERMAFVSEDRRGVGIMAEESIELNIVLPSVRVQRRFLKQVAPLLHLLDQSGVRR
                     HAEEMIRRLDVRTTGPTQPVGRLSGGNQQKVCVARAMTLDPEILLVSEPTRGIDIGAK
                     KLVLDLIVELNRTRGVTVIVTSSELAELRSICDRIAIVTRGKVHAILEPDAADAQYGL
                     AMAGEPMSA"
BASE COUNT          768 a         1373 c         1618 g          656 t
ORIGIN      
        1 agcgtggtgc ccgagatgtc gctgaagaag atcctggcgc ccttggccat gccgtcgcgc
       61 aggtcggcgc caccgacggg tgaatcgtcg ccggccacgg tgccgttggt gtgcgtaccg
      121 tggaacgagg cgcccgaatg gtcgccgaag gtgatggccg ggttattcga gcccaggtgg
      181 tacgcgatga tcttccggtg ggtcggataa tcgccgaacg tggtgatcgg cacccccgca
      241 tcgcggaact ggttgtgtgc ggtcgtgatg ccggaatcgg agttgtggac cacctgcccg
      301 gtgccgtcga tcccctggtc ccagatcttc cggttgctgt tgacgttggt ctggacgacc
      361 cactgggcgt tgacgttctc gaaggtgggc gtgatccagg gctcgaccca ctgcacgccg
      421 ggaagccggg cggcctcgag ggcgcgcgcc gccggcatcg tcacgcggag gaggcgattg
      481 atgccgttgt cggattcggc ggtcacttcg gcgcccaggt tcacgaggcg ttgcctgtcc
      541 cggccgaccg gcgcgtcgtt gaagaggagc acggtcatct cgacaggcac ggcggcggcc
      601 gcggcgtttc cttcggcctt tgccttgagc gcgggggcca ggcgataggc cgggtgccag
      661 tcgccggtcc aggtgacccg cgcgccgcgg gcaagggcgc tccgcgattc ggggaagatg
      721 cggacgagcc aggcatcgcc ggccagcggg gttaccggca cggcgcccgc cgcctggagc
      781 tgggccaggt cgtccggcgt ggtcgggccg tcgaactgga cgatgacgcc gacgcgttcg
      841 tcgttctccg cggtaacgcc gctcttctgc gccgccgtga acgccggttc gccgttggtc
      901 ggatcgaagt cgtagccggc cgccaggtga aggttgcgat cacgctggcg ggtcatcgtc
      961 ggcgccagcc ggccccggga ttcgctgcgg atgagcggcg aaccaccgtc cgacacctgc
     1021 cacgacggtc ggacctcgac ccgacgcacg ggactgaccg cgaacgagcc gccgtctccg
     1081 tcatcggagg ccacggccca gtaccagccg gactcgagcg cggtgaggcc cagggagacg
     1141 ccgtccacca cgggagtcgt caccgggaaa acacggatac cgtctccctc ggggagcttg
     1201 tcgagaccgt ccgtgctgaa cggttcgcgc gagacgagga ggaactggcg cgtggtgccg
     1261 ggggccggca cgaaagaaaa gcgaagaccg gcctcggtga ccacttcacc atcgaagggt
     1321 ccgaccagtt cgggacggcg cacgattcgc tgccccttgc cgtcgacgga tggcgccatg
     1381 aggactgcga ggccgaacgc ggcgaccagg aggaggaggg gtcgagtccg gcgggaatga
     1441 gcgatcacgg tggcaagggc ccgatgcatg aatcctccgt cggggtggga tggatgttcg
     1501 ggggaggcgc tgatcctgtt gaagcgcttc aattctaacg gactggcgaa tccggatcga
     1561 gcgcaaaccg ggtgcggcgc gaaatcgggg cgaaatgacc ctacccgggc gtcaggcggc
     1621 tttcggcttc cggcgtccct gccaggcgtg gaggacgagc gccagcgtga tgaccgcgta
     1681 ggccacgaag acgcggaagt actcgccgat ctgggcatcg cccgccaggg ccttgccggc
     1741 caggggtgag accacgaaga gggtatggaa aaggagggtg ccgaccaggg cctgggccac
     1801 ggtggccttc ttgaccgtgg cacccgacac gagcagggcg gcgatggcga acatgccgac
     1861 ctgctcgtgc gagttgtagg tgttcatggt gccgagattt tgcaggaaaa ggagctggcc
     1921 ccaggcgccg agcaccgtgg acaggatcgt ggcgatgatg cggttccggt cgaccgggat
     1981 gccggcgatg gccgcgacgt gggcatcctg tcccatggcc ttgagttgct ggccgagctt
     2041 ggtgcgaaga aatgccacga tcaggaaaca gaagacgacg acgaccagta tcgtggccac
     2101 cggcacgcgg acgttgccgc cgaagatggc cagcttgaga gggatcaggt cgtcgagcgc
     2161 gtactggaga ccggtgaggt cgaccgtgtt ccggaggccg tacccctgcg ggaggatcat
     2221 cgcctcgttc ttgaagggaa tgagggtgcc ggccaggaag agccagacga actggtagat
     2281 gccgttggcg aagtagccgg cgatgaggcc ggtgatcatc tcccgcccct tggccatgtt
     2341 gaagagccag ccgaccagcc agccaaacag gatggccagc ggcatggcga tcagcgccgc
     2401 gaggccgaaa ccgcccagac ccttcacgcc ccagtgggtg acgaggatgg ccgctacctg
     2461 gccggccatg gcgccgatcg tgatgccgaa gttgaggccg agccccgcca ggatcgggat
     2521 gatgagcgcc agcacgagga gggagttgcg gccgaagcgg atcgacatct cggtggccag
     2581 gaagaggggc tcgatccgtg ccaccaggat gccggcaagg cagagtccga ggaagagggc
     2641 cgggaccgcg aaccgcttca tcccttgtcc ccgcgccggg tgagcgcgta gaggatcatg
     2701 ccgttctgga tgacgaggcg ggcggtttcc gacacgtcgc ccaccaccac tttgctggtg
     2761 acgggaagcg cgatcgtcag gatcgtctgg aagaggaacg tgccgacgac gacgttccgg
     2821 atgctggccc gcatcacgct ggcgccgccg atcaggatgc aggccaccgc cgggaacgcc
     2881 atcatgagcg gagcctggta gagctgcacg aagccgtacg actggctgaa ggccacgatg
     2941 ccgaccgcgc ccagcaccgt ggtgaggatg ctggcgtgta cgcgggtgtt ggcgtcctgg
     3001 atgccgctcg agcgggcgaa gcgggggttg tcgccggcgg cgctgatcga gaggccggca
     3061 cgcgtgcgga agaagagcca caccaggagg cagaagaatc cgaagtagag gagggtgccg
     3121 gtcggcagga cgaagtcgcc gatccggacc tggaggaact tgtcgaggat cttctcgaac
     3181 gtgtcgttca gcgtgagggt ggtgcgcaat cccttgccgc cgatcgccca gacgagcttc
     3241 gggttgtgga acggcgcaag cagccagaag atcgacatga ggctgaccat ggcgaagccg
     3301 acgtaggtgc cgaccatcat ctcctggccg cgcgtacggt tcaggagcca gccgtaaatg
     3361 ccgccgatgg gcaccgcgag gagcgaaccg accagcgtgg ccaggaagaa cccgggccac
     3421 cccgccgcgc cgaagttgat caccgacacg catccgacga gtccgcacag gatgccgagc
     3481 ggcaggccga agttgagccc gagcccaccc tggatggccg gcaggagcgc gagcacgaga
     3541 atgccgttcc gggccacgcg gtcgatgctc tgcgagagaa ggctggccag gttcatgccg
     3601 gtgacggccg cgatcaggaa gaggaggacg aggaagccga gaatgatgag gcgggggacg
     3661 cctgcctcgc gcacgaactc gcgccagcga tccccggccg tcgagcggga gagcgtttcc
     3721 ttcggcgcgc tcatgcggac atcggctccc cggccatggc caggccgtac tgggcgtcgg
     3781 ccgcgtccgg ttcgaggatg gcgtggacct tcccgcgggt gacgatggcg atccggtcgc
     3841 agatgctccg caactcggcc agctccgacg acgtgacgat caccgtgacg ccgcgggtcc
     3901 ggttcagttc gacgatgaga tcgagcacca gcttcttggc cccgatgtcg atgccgcgcg
     3961 tcggttccga cacgaggagg atctcggggt cgagcgtcat ggcgcgcgcc acgcacactt
     4021 tctgctggtt gccgcccgac aggcgcccca ccggctgcgt ggggccggtc gtgcgcacgt
     4081 cgagccggcg gatcatctct tcggcgtgac gccggacgcc cgactggtcg agcaggtgga
     4141 ggaggggcgc cacctgcttc aggaagcgcc gctgcacccg gaccgacggc aggacgatgt
     4201 tcaactcgat cgattcctcg gccatgatgc ccacaccgcg ccggtcctcc gacacgaagg
     4261 ccatccgttc gccgagtgac gcgccgggat cgttgagctt gagcggccgc ccgcgcaccg
     4321 tgatggcgcc gcgggccggg tggaggccca tgatgccgtt ggccacgcca accttgccgt
     4381 agcccgccag ccccccgagg ccgaggattt cccct
//
LOCUS       DASVOD010000277         6522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_227729, whole genome shotgun sequence.
ACCESSION   DASVOD010000277 DASVOD010000000
VERSION     DASVOD010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6522
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227729"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VF720_06045"
     CDS             complement(<1..746)
                     /locus_tag="VF720_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX7878951.1"
                     /translation="MTHDPELDLLGEALALLRRGFTHLPPVDGVDPADLAPFRSALLA
                     AAERMQDNFPYHHPLYVGQMLKPPHPVARLAYAMAMHVNPNNHALDGGRASSAMEKEA
                     VAAIARMFGWSSALGHLAGGGTMANFEALWVAREMSRETHGDRAGAVAASSQAHYTHS
                     RLSAVLDMPLVAIDVDEAGRMDMSALEAALDRGERGEGPRIDTVVAALGTTAIGSVDP
                     LASILAMRERRHKAHGRTLRVHVDTAYGGY"
     gene            complement(743..1666)
                     /locus_tag="VF720_06050"
     CDS             complement(743..1666)
                     /locus_tag="VF720_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7878952.1"
                     /translation="MSNDRFPAHLISIEFADSPTPVDTVRTLFREYADAIGTDLEYQG
                     FSAELATLPGAYAAPDGALLLVSVEGEPAGCVAFKRLDEHGAELKRLFVRPAYRALGL
                     GRWLVGNVIDLACVAKYRTLRLDTLAHMTGAQNLYRRIGFREIAPYGDGHMPGTRFFE
                     LKLYRMSVPVFRLSSEWLERYRALRFDPEAERHMSVIPYETLIWTDEHPGGEGDVPEL
                     DETSHATIFRLITTRSTFWSSGDVPLICRQFWEDAKATLPDWPGFRRLSVSKDEMAMM
                     KQWSNDLDRAERACERLERLQKRASRKAARQ"
     gene            complement(1720..3402)
                     /locus_tag="VF720_06055"
     CDS             complement(1720..3402)
                     /locus_tag="VF720_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX7878953.1"
                     /translation="MRRRPGRFVIRFLFLAMTAAAGLSTNRRALASDDDIGLDAITAE
                     RIRRHVAVLASDAYGGRLPGTAGEDSTLAYLEREYRALGLEPAVNGSYRQRVPLVKRT
                     VLPTPTMSLHRIGNAKGAVPSIAWGEDLTIGSNRLDETLALHDAELVFVGYGITAPEL
                     GWDDYAGVDMKGKVALALSADPGRVPADSNLFGGRGLTHYGLSQTKQEMAAAHGAVGV
                     ITMHDQEIVGYPYNLLTASAGRGRYDLVASGDALPKTPFGGTFRKEIVQAILTAAGHD
                     PAVLMESAGRRGFRAVPLGIALDLEARLRIERSESYNFLGLLRGREHPDEVVAYSAHW
                     DHVGIGVPVDGDSIYNGAIDNATGTAALLTLAKAYRSRPEPPARSVLFWATTCEEQGL
                     LGSYHYADNPVVPLNKTVALINMDALFPFGKVGAIVVTGYGQTELEDLLVEASKPEDR
                     VVLPDDNPEAGAYFRSDHYPMAKKGVPSIFGVGNPVDDSDSTLMARFTGYVQTGYHKP
                     KDELTDDWDLAGIVQDVRSFYRLGAGLADGTAWPNFRATSEFRHLRDAMMRR"
     gene            complement(3526..4482)
                     /locus_tag="VF720_06060"
     CDS             complement(3526..4482)
                     /locus_tag="VF720_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878954.1"
                     /translation="MRRQLAIAMLAVLLAGRPAPEAEASTQIGDPKPPPAPTAPDSTA
                     APDAHLDRPKTEAKTGGKAGALAGAVAGGGLFLLGAAYSDAETGDVDAAGYVGIGLLG
                     AALGGIFGWALGSFLGSTVPAERPATTHHVTATPPASENAPAPTLEPPTAMPASSRHG
                     RTLASFTLLGGYAGMADEGPVYEYPPEGGFVVHEGGTVGGSAFLGARLLRHRGEWFSY
                     GPEVGFVTGHDGMFLLTADLLVGRRYGSTRPYAIGDIGWHNWKDGASLFGGSIGAGLE
                     WEREPGATAFGAEYRYRAMLQNDTGEGDREFHQLALTSRFNW"
     gene            complement(4483..5109)
                     /locus_tag="VF720_06065"
     CDS             complement(4483..5109)
                     /locus_tag="VF720_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014197072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX7878955.1"
                     /translation="MDIALAIAPILAVLAIGVISPGPSFILVGRTAVAVSRRAAVASA
                     FGMALGATLLAIAALFGLNAIFQKIPAAFLALKIGGGLYLLYLAVRTWRSADKPIALD
                     GVLDEADGAPLRHFGLAIATMLSNPKAAIQYGVIFAAMLPKSPSLALSLALPPSVFLL
                     EVSWYLVVAFALSADGPRGTYLRARSVLDRVAGAILGLLGIKLILSSR"
     gene            5262..>6522
                     /locus_tag="VF720_06070"
     CDS             5262..>6522
                     /locus_tag="VF720_06070"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7878956.1"
                     /translation="MPDPAGSITPALSARQESIDIMTGKPGVVPLVARSSHSLLMGTT
                     AIDALVEQAARLGHPALALTDRNNVYGLVAFRTAARGAGLAPIHGAEVDDPSTGTRAV
                     LLVRTMAGYENLCRILTRRMMEADFALGAALAVPGATDGLHVLTDDDALVRALHPVLE
                     PNRLWILLADPSRAPNDWRRLATIGRELKRPLVASPDVMYLEEADRGQHRLVTAIRRR
                     ELVSRVPEEALAPPGSHLPDSRRIIERFRDFPEALVNNRLIREECADLDWTLGKPIFP
                     KCPLPPGETAYSHLFRMCLDGMRRRYGGLHPPAMRRLAQELEVIDRLGFPEYFIIVAD
                     ICHFAHGQGIPTVGRGSGAGSIVTYLLGITNVCPLRYRLYFERFLHDKRRDLPDLDID
                     LCWRRRDDVIDHVYRTYGADRVAMICTH"
BASE COUNT         1028 a         2249 c         2225 g         1020 t
ORIGIN      
        1 aagtagccgc cgtaggcggt atcgacatgc acgcgcagcg tgcgaccgtg tgccttgtga
       61 cgtcgttcgc gcatcgcgag gatgctggcc agcgggtcca cggagccgat cgccgtcgtc
      121 ccgagcgccg ccaccaccgt gtcgatccgg ggtccctcgc cccgctcgcc gcgatcgaga
      181 gccgcctcga gcgccgacat gtccatccgt cccgcttcgt ccacgtcgat cgccacgagc
      241 ggcatgtcga gcaccgccga cagccgcgaa tgcgtgtagt gcgcctgcga agaggccgct
      301 accgcacccg cgcgatcgcc atgagtctcg cggctcatct cccgcgccac ccacaatgcc
      361 tcgaagttgg ccatcgtccc gccgccggcc aggtgtccca gtgccgacga ccagccgaac
      421 atccgcgcga tcgccgccac cgcttccttc tccatggccg aactggcccg tccgccgtcc
      481 agcgcatgat tgttcggatt gacgtgcatg gccatcgcat acgccagccg cgccaccggg
      541 tgcggcggct tgagcatctg ccccacgtac agcggatgat gatacggaaa gttgtcctgc
      601 atccgttcgg cggcggcgag aagcgcggag cggaaggggg caaggtcggc gggatcgacg
      661 ccgtcaaccg gcggcaggtg ggtaaagccg cggcgcagca gggccagggc ttcgccgagc
      721 aagtcgagtt cgggatcgtg cgtcactgcc gggccgcctt ccgactcgcg cgcttctgga
      781 gtcgctccag tcgctcgcag gcacgttccg cgcggtccag gtcgttggac cattgcttca
      841 tcatcgccat ttcatccttg gagacgctca gccgacgaaa tcccggccag tccggaagcg
      901 tggctttcgc atcctcccag aactgccggc agatcaatgg cacgtcaccg gatgaccaga
      961 aggtcgagcg cgtggtgatg aggcggaaga tggtggcgtg tgacgtctcg tccagctcgg
     1021 ggacatcccc ttcgccgccg gggtgttcgt ccgtccagat cagggtttcg taagggatga
     1081 cggacatgtg ccgttcggct tccgggtcga atcgcagcgc gcgatatcgc tcgagccact
     1141 ccgacgagag tcggaaaacc gggacggaca tgcggtaaag cttgagttcg aaaaagcgcg
     1201 ttccgggcat gtgcccgtcg ccgtagggag cgatctcccg gaacccgatc cgacgataga
     1261 gattctgcgc gccggtcatg tgggccaacg tatccagcct caatgtgcgg tacttcgcca
     1321 cgcacgccag gtcgatcacg ttccccacca gccagcgtcc gagcccgagt gcgcggtatg
     1381 ccggccggac gaagagccgt ttcagttcgg cgccatgctc atccaaccgc ttgaaggcca
     1441 cgcaccccgc cggttccccc tcgacggaca cgagcagcag cgccccgtcc ggcgccgcat
     1501 acgccccagg caacgtcgcc aattccgcac tgaatccctg gtactcgagg tcagtcccga
     1561 tggcgtcggc atactcacgg aagagcgtgc ggacggtgtc gaccggggtg ggggagtcgg
     1621 cgaactcgat cgaaatgagg tgtgcgggga aacgatcgtt cgacatggga ccggaagtct
     1681 acgcccatgc ccgacgcgcg tcaccgggcc cgcgggccgt caccgccgca tcatggcatc
     1741 cctgaggtgc cggaattcgc ttgtcgcgcg gaaattgggc caggccgtgc cgtcggccag
     1801 tcccgcgccg aggcggtaga acgagcggac gtcctgcacg atgccggcca ggtcccagtc
     1861 gtcggtgagt tcgtccttgg gcttgtgata gccggtctgc acgtagccgg tgaagcgcgc
     1921 catgagggtg gaatcggaat catcaacggg attgccgacg ccgaagatcg aggggacgcc
     1981 cttcttcgcc atggggtagt gatcgctccg gaagtaggcg ccggcttcgg gattgtcgtc
     2041 gggcaggacc acccgatcct cgggcttcga tgcttccacg aggagatcct cgagctcggt
     2101 ctgtccgtag ccggtcacga cgatggcgcc caccttgccg aaggggaaga gggcgtccat
     2161 gttgatgagc gccaccgtct tgttcagcgg cacaacggga ttgtcggcgt agtgatacga
     2221 accgagcagc ccctgctcct cgcaggtcgt ggcccagaag agcacgctgc gggccggggg
     2281 ctcggggcgc gatcggtagg ccttcgccag cgtgaggagc gcggcggtgc cggtggcgtt
     2341 gtcgatggcg ccgttgtaga tggagtcgcc gtcgacgggc acgccgattc cgacgtggtc
     2401 ccagtgagcc gaataggcta ccacctcgtc gggatgctcg cgcccgcgca gcaggccgag
     2461 gaagttgtac gactcggagc gctcgatccg gaggcgcgcc tccaggtcga gggcgatacc
     2521 gagcggcacc gcccggaagc cgcgacgtcc ggccgattcc atcagcacgg ccgggtcgtg
     2581 tccggcggcg gtgaggatcg cctgcacgat ctccttgcgg aacgtgccgc cgaacggggt
     2641 cttcggcagg gcgtcgccgc tcgccacgag gtcgtagcgg ccgcggccgg ccgaggccgt
     2701 caggaggttg tacggatagc ccacgatctc ctggtcgtgc atcgtgatga cgcccaccgc
     2761 gccgtgcgcg gccgccatct cctgcttggt ctgcgacagg ccgtagtgcg tgaggccgcg
     2821 tccgccgaag aggttggagt cggccggaac gcggccgggg tcggcgctca gcgccagcgc
     2881 cacctttccc ttcatgtcga cgccggcgta gtcgtcccag ccgagttcgg gggcggtgat
     2941 cccgtagccg acgaagacga gttcggcatc gtgcagcgcc agcgtctcgt cgaggcggtt
     3001 cgagccgatc gtcaggtcct cgccccaggc gatcgacggc acggcgccct tcgcgttgcc
     3061 gatccggtgc agcgacatcg tgggcgtcgg cagtaccgtc cgcttcacca gcggcacgcg
     3121 ctgccggtac gagccgttca ccgccggctc gaggcccagc gcgcggtact cgcgctcgag
     3181 ataggccagc gtgctgtcct cgcccgcggt accggggagg cgcccgccgt acgcgtcgga
     3241 agcgagaacc gccacgtgtc tgcggatgcg ctcggcggtg atcgcgtcga ggccgatgtc
     3301 gtcgtcggag gccagcgcgc ggcgattcgt ggaaagccct gccgcggcgg tcatcgccag
     3361 gaataggaaa cgaatgacga accgcccggg ccgacgccgc atgccaaacc ccctcgtgcc
     3421 gatggacggc cgcaccgcgc gaccgccccg cacagacgga ggaagaaggc tgaccgttca
     3481 ggcgtttcgg tcaggatccg gaaacggtgg gatgtggccg ggcggttacc agttgaagcg
     3541 gctggtgagg gcgagctggt ggaactcgcg gtcgccttct cccgtgtcgt tctggagcat
     3601 ggcgcggtac ctgtattccg ccccgaacgc ggtcgcaccc ggttcccgct cccactcgag
     3661 gccggccccg atgctgccgc cgaagaggct ggccccatcc ttccagttgt gccagccaat
     3721 atccccgatc gcgtacgggc gggtcgagcc atatcgccgg ccgaccagga ggtcggcggt
     3781 caggaggaac atgccgtcat gcccggtcac gaagccgacc tcgggcccgt acgaaaacca
     3841 ttcgccgcgg tggcgcagca gccgggcccc gaggaacgca ctccccccca cggtgccccc
     3901 ctcgtgcacg acgaatcctc cctcgggcgg atattcgtag acgggccctt cgtccgccat
     3961 gcccgcgtag ccgccgagta gcgtgaacga ggccagcgtg cgtccgtgac gtgaggaggc
     4021 gggcatggcg gtgggcggtt cgagggtcgg agcgggagcg ttctcggagg ccggaggcgt
     4081 tgccgtcacg tggtgcgtcg tggccggccg ctccgccggg accgtcgatc cgaggaacga
     4141 cccgagggcc cagccgaaga tgccgccgag cgcggcgccg agcaggccga tgccgacgta
     4201 gccggcggcg tccacgtccc cggtctcggc gtccgagtag gccgccccca ggaggaagag
     4261 ccccccgccg gccaccgcgc cggcaagggc tccggccttg ccgccggtct tcgcttccgt
     4321 tttcggacgg tccaggtgcg catcaggggc cgccgtcgaa tccggtgccg tcggtgccgg
     4381 gggcggcttt ggatcgccga tctgggtgga cgcttcggct tccggtgcgg ggcgcccggc
     4441 cagcaggacc gcgagcatcg cgatggccaa ctggcgtcgc atctagcgtg acgagaggat
     4501 cagcttgatc cccaacagac ccaggatcgc gccggccacg cgatcgagca ccgagcgcgc
     4561 ccggagatag gtgccgcgcg ggccgtccgc ggacaacgcg aaagccacca ccaggtacca
     4621 cgagacctcg agcaggaaga ccgacggcgg cagggccagc gagagcgcga gcgacggcga
     4681 cttcgggagc attgccgcga agatcacgcc gtactggatg gccgccttgg gattgctgag
     4741 catcgtggcg atcgccaacc cgaagtgtcg gagcggcgcg ccgtccgcct catcgagcac
     4801 cccgtcgagc gcgatcggct tgtcggcgct ccgccacgtc ctgacggcca ggtagaggag
     4861 atagaggccg ccaccgatct tgagcgccag gaatgccgcc gggatcttct ggaagatggc
     4921 gttgagcccg aacagtgccg cgatggccag cagcgtggca cccagcgcca tgccgaacgc
     4981 cgaggccacc gccgcgcgac gcgacaccgc cacggccgtc cgccccacca ggatgaaact
     5041 cggccccggg ctgatgaccc cgatggcgag caccgccagg atcggcgcga tggcgagggc
     5101 gatgtccatg gtcggtcgtc tccgggtgtc gtggcgccgg cgaaacgccg gatcgtcgcc
     5161 ggtgaaggat acatttcccc gttgcagggc gcccccgcat gccgctcccg aacggttgct
     5221 ttttcgaccc ttgactacca ttcatttgtt tagtattttc catgcctgac cccgccggat
     5281 ccatcacgcc ggccctttcg gcccggcagg aaagcatcga catcatgacc ggcaaacctg
     5341 gcgtcgttcc gctggtggcc cgcagctcgc attcgctgct gatgggcacc acggccatcg
     5401 acgcgctcgt cgaacaggcg gcccggctcg gccatcccgc gctggcgctt accgatcgca
     5461 acaacgtgta cggcctcgtg gcgttccgga cggcggcgcg gggcgccggc ctcgcgccga
     5521 tccacggcgc cgaggtcgat gacccctcca ccggaacgcg ggcggtgctc ctcgttcgga
     5581 ccatggcggg ttacgaaaac ctctgccgga ttctcacgcg gcggatgatg gaagccgatt
     5641 tcgcgctggg cgcggcgctc gccgttcctg gcgcgactga tggcctgcat gtcctgacgg
     5701 acgacgacgc gctggtgcgt gcgctgcacc ccgtcctcga accgaaccga ctctggattc
     5761 tcctggccga tccgtcgcgg gcgcccaacg actggcggcg cctcgcgacg atcggccggg
     5821 aattgaaacg accactcgtg gcgtcgcccg acgtgatgta cctcgaggag gcggatcgcg
     5881 ggcagcatcg cctggtcacg gccattcgcc ggcgcgagct ggtgagccgc gtgcccgagg
     5941 aggcgctggc gcctccgggg agccatctcc ccgactcccg ccgcatcatc gagcgcttcc
     6001 gcgattttcc ggaagcgctg gtcaacaacc ggttgatccg cgaggagtgc gccgacctcg
     6061 actggacgct cggcaagccg atcttcccga agtgcccgct gccgccgggc gaaacggcct
     6121 actcgcacct cttccggatg tgcctcgacg gcatgcgccg gcgttacggc ggcctccatc
     6181 cgccggccat gcggcggctg gcgcaggaac tcgaggtgat cgaccggctc ggcttccccg
     6241 agtacttcat catcgtggcc gacatctgcc acttcgcgca cggccagggc attcccacgg
     6301 tagggcgggg gagcggggcc ggttcgatcg tcacctatct cctcggcatc acgaacgtct
     6361 gcccgctccg ctaccgcctc tacttcgagc gcttcctgca cgacaagcgg cgcgacctcc
     6421 ccgacctcga catcgacctc tgctggcggc ggcgcgacga cgtcatcgac cacgtgtatc
     6481 gcacctacgg cgccgaccgc gtggcgatga tctgcacgca ca
//
LOCUS       DASVOD010000278        11188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_748861, whole genome shotgun sequence.
ACCESSION   DASVOD010000278 DASVOD010000000
VERSION     DASVOD010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748861"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VF720_06075"
     CDS             <1..638
                     /locus_tag="VF720_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878957.1"
                     /translation="AGSLSGWLRYQLGTFTLSAPGWGLPDSAQTTSEPGTLKYLAQVG
                     ATDVSREWRAVEHPDLPGQRVEVGGFDPLVGLVPPRSSVDSLAMKTGNFARSVLGWMP
                     RLEIARAETRTLGDGLYRVTVEIVNTGWLPTALEVGVTTRKARPVRAEIVGQDGVTVV
                     GGPPVKLIPTISGSGGRERIEWIVEARSGAKIVVRAATPRAGESRREVTLR"
     gene            635..2446
                     /locus_tag="VF720_06080"
     CDS             635..2446
                     /locus_tag="VF720_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7878958.1"
                     /translation="MTTLSLKFTRAALAVALTTLAIAPLTISPLAIPTARADSGRPAV
                     LPAIGGLGVPANPKVYLRWDAFHDVAGLGAALKAIADAHPDLARFGSIGKSYEQRDLH
                     LITITSFKTGGDTEKPAIWIDANIHGNEIQGGEVALYTAWYLTEMYGQVPEITELLDR
                     VTFYIVPSINPDGRDGFIHEPNNRHSSRSGKRPYDNDNDGLVDEDGPDDLDGDGNICQ
                     MRIKDPFGRYKVDPDDSLNMVPVKPGESGSYTLLDDEGIDNDGDGRINEDGLGGYDPN
                     RNWPVNWQPTYLQNGAHEYPGSLPETRAILDFVYAHPNIGAAQSYHNAGGMILRGPGQ
                     ESLTYEGEDITAFDTIGNAGEEILPGYRYLVTWKDLYPVSGGELDWFYLGRGVIAFTN
                     ELFTAFNYFRDPDLRENGFDDKPESKFDKLVLMREASVTWKPYDHPQYGPIEVGGTKK
                     NFGRVPPGFLLPEECHRNMAFSLYHAMQLPKLVIGRPVVTPVPGGLKRIRVTVANESA
                     IASRTARDDRMKITPEDRATLSGGLTIVSSGLLRDRYGSVVTPASRTEPAAALRIGRV
                     PGFGAVTMEWLVRGSGDATVTIQSTRGGTVRRSFRVP"
     gene            complement(2398..2769)
                     /locus_tag="VF720_06085"
     CDS             complement(2398..2769)
                     /locus_tag="VF720_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878959.1"
                     /translation="MFQSIAPALEFVILSLVPAAAVTAYWILARTPAEPATFESRQAR
                     LFGWFLWVGLVLISAVGMSVTAGPTGTEGNAASAGHGWKTAAALLPLIGLSVAEGVTW
                     LRRGAAYGTRNERRTVPPRVD"
     gene            complement(2934..5768)
                     /locus_tag="VF720_06090"
     CDS             complement(2934..5768)
                     /locus_tag="VF720_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7878960.1"
                     /translation="MPPPGSTLGPYRLEALLGAGGMGEVYRALDTRLGRSVAIKVLSR
                     HLAATAEARARFEREARTVSQLNHPNIATLFDVGQEGDTDYLVMEFLEGETLADRLVR
                     GPLPTAEFLALAGPIVDALAAAHRAGVIHRDLKPGNVMLTAGGPKLLDFGLARPLEGV
                     VTDASSSQSPTVNAPLTGQGVIVGTLQYMAPEQLEGREVDARTDVFALGCVLFEMATG
                     RKAFAGDTNLSIVGSILHAAPTRLHQGADDLPGAVRSLIERCLARDPAARPADGGMVA
                     AELAGLGDWVRLTALPKLAALVDGAMRIEERSGCWEAWHLAREVERLTPDDPLLARLW
                     PEFTNPVTFESEPPGAAVTVQFYGTPGEPWVPLGTTPITVARMPRGIYRVRLEARGVE
                     PVEDVIWNIDFGMSGASDPSACLWRYVMRPTDELPEGMVEVPAGQFPLFMPGLDHLDA
                     EAIDRFWMDRHPVTNRRYREFVAAGGYREPSHWRHDFMTADGPIPREAAMARFVDTVG
                     APGPAGWEFGEFPAGEDDHPVTGISWYEAAAYAAWRGCDLPTIFHWNWVAFTVASSQI
                     VPRANFGGKGLMPVGATTSMNRFGVHDLAGNVREWALNDADRPGERFILGGAWSDADY
                     TFTDAVTRNSLDRSEGNGFRCIRRNGVDPNAARLERTIDMPFRDFRKEEPVPDAVFEF
                     FRRQFQYDRTPLNARIVADEPGPFSRRQTVEFDAAYGRERVTAYLYLPENSRPPHQAV
                     VIFPGSFALHTAEFTDAEIRRADYILKSGRALIHPVYKATYHRRDGFKSDYPSESADY
                     RDHVIMWAKDLSRTIDWLATRDDIDAEKLAYFGLSWGGALGAILPADEPRFKAVLLYV
                     AGFCFQRCLPEVDAVNYAGRVRQPVLMLNGEHDFFFPLDTSQRPLFEALGAPPGQKRQ
                     VIYPGGHTVPRTEIVKEVLAWLDRYLGPVG"
     gene            complement(5895..11159)
                     /locus_tag="VF720_06095"
     CDS             complement(5895..11159)
                     /locus_tag="VF720_06095"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7878961.1"
                     /translation="MQDVYTIPTALGGSFAAGYAGDYYQIAAYNGRAFAAWADNRTSQ
                     YNAYWDEIVYADPDDPNAPSNIIATSDCTTPTSVTLDWDDPTTRVNGTPLGNFEIRIT
                     RLGGPTVDVDQGIETYTDAGLTTGTLYTYELRTHDEDNDSLSIVATITKYAGGDPTPA
                     PPTLFTVGAIPGPAFDLDWTNPTVNIDGSQLCDFDGVNVYRNGAYLTTVSFAPGDIGL
                     PGTYSDLAPVPGLNCYKLTAIDSEIPLHESAFTTEICVEVPLNPPFSDCFDVAGAPDP
                     GKWTTDGAVVDGLGSNPPTPPNSLDLDGQDVVTSRAIDMGAASGSGYVLLYQKEQTGN
                     GESPDPTNDLVIELYNSNGVWDEVNRHLGVDGDEVSFTTHVLALDSLVPSSGTYFHSQ
                     FRFRFRSTGDPGFDNWFVDCTTLDIPSCQATATVAPTSVDDTLVAGDPTGDADVVTVS
                     NSCQFNALTWSAAESPDVSWLVLTGASGSISGLGSQNFVATFNATAMAPGVYNTDIVV
                     TTNDPVNPTITVTATLTVTDAPDISVSPTSITNTLANTDPDAHDLVAINNVGTGPLEF
                     FIRDASSAVRSWAGPQFVGVKRADGTVLPMAGAPAPTPSQVKAAWYAAELGDEGRRSA
                     RIDGGLDPAAELSGDRVRSRDRLKNLGDRGQNVHYPPQPFAQGGPDAAGYRWIDSDEA
                     NGPLFNWVDISGIGTDTGITGDDQNLGPFALGFAFNHYGTSFTGIRVCSNGFLSFTSS
                     ATPFTNTGVPNAAAPSDLVAPFWDDFNPANAGTIYYYQDVANNRFIVQYDAVSYYADD
                     LSSNTFQVIINKDGTIVFQYLDIDSPSNAGTVGLQNAAGTVGLQVAFNQAYVHNELAV
                     LLYQDATWLSATPGSGVVPPGDSAMLDVVSDPTGLAPGTYYGNILIQSNDPDEPVTDV
                     HVELNVTGNGAALPFCDDFNSGAAPDAARWTNSGVVLNATGINEPSLPTSADLNGTDL
                     MTSIQLDLSTAGGTGKTLIYFQEAGGGGDPPEAGNDLVVEFRNSSGVWDQVDRVFGGT
                     GAATNYGVRIIDLDGLTPSSGTYFYPLFQLRIRTTGEAATDDWFVDDLCLSNPPCQPT
                     ISVNQTSVCDTLVAGSIGLPKSLGIANSCGYANLVFNIIESPDQPWLTVIPTNGVIAG
                     ATSTSVSLNFDATGMLPGTYNTTLFVTSNDPVTPSVPVAICLRVTGAPRAEVDPDSIC
                     PVVGIFGGPVIHRAASIQNTGVVPLTWRLSDTQGIPAAPNALDPTSTRRFGDPVPNQK
                     KLELFKEVKDLVMPEYEAGTVGNDPEAKGYRNPFLGGFQPYGQGGPDAFGYRWIDSDQ
                     AGGPEFMWIELQGNGGIDTGLHADDVTTAPIAMGFNFPFYGTNFNSVRVCSNGWMSFT
                     STATAFTNTALPNTAQPNNTIAPLWDDYDMRNGVGTCYYRSDVALGRFIVEWKKVTRF
                     NALSDTLTFQAILCKDGSITYNYLSVKGILNSNTIGIENAAGTTGLQVVFNANYVHNN
                     LAVLIQNEQEWLSATPTSGTIPPGGTQLINVAFDPTGVAFGMHRGFLRITTNDPVLPK
                     ATIPVCFSVEDATAVELARFEALSTPDGVKLAWETTEEVEHSHFDVYRAEGLSSDFVQ
                     VNTSPITGRHAYEFTDAAVVDGAEYRYRLEAVDRFGGRVTYGPVVVTYAGRPAVFNLV
                     QNYPNPFRGNTTFLLAMPVASDVHLRIFDAAGRLVRNVHDGAMDAGRHTFEWNGRDDA
                     GSPAAAGFYFLRAESTQGTKVVRMMLVK"
BASE COUNT         1868 a         3593 c         3903 g         1824 t
ORIGIN      
        1 gtgccggatc gctttccggc tggctccgct atcagctggg aacgttcacc ttgagcgcgc
       61 cggggtgggg ccttcctgac tccgcgcaga ccaccagcga accgggcacg ctcaagtatc
      121 tggcacaggt cggtgcgacg gacgtgtccc gggagtggcg ggcggtggaa catcccgacc
      181 tgccgggaca gcgcgtggag gtcggcgggt tcgatcccct ggtcgggctc gtgccgccgc
      241 ggtccagcgt cgacagcctg gcaatgaaaa ccggcaactt cgcccgctca gtcctcggct
      301 ggatgccccg gctcgagatc gcccgggccg aaacacgcac gctcggcgac gggctctacc
      361 gggtgaccgt ggagatcgtg aatacgggct ggctccccac cgcgctcgag gtgggcgtga
      421 cgacgcgcaa ggccaggccc gttcgcgccg agatcgtggg gcaggacggc gtgacggtag
      481 tgggcggacc gccggtgaaa ctgatcccga ctatcagtgg ctcgggagga cgtgaacgga
      541 tcgaatggat cgtggaagcc cggtccggcg caaagatcgt ggtgcgcgcc gccacgccgc
      601 gggcgggcga gtcccgccgg gaggtgacgc tgcgatgacg acgctgagct tgaaattcac
      661 ccgcgccgcg ctggcggttg cgctgacgac tctcgcgatt gccccgttga cgatttcgcc
      721 actggcgatt cccaccgccc gcgccgactc cgggcgcccc gccgtcctcc ccgccatcgg
      781 ggggctgggc gtgccggcca atccgaaggt gtatctccgt tgggatgcct tccacgacgt
      841 ggccggcctc ggcgcggcgc tcaaggccat cgccgacgcg catcccgacc tcgcgcgctt
      901 cggatcgatc ggcaagtcgt acgagcagcg cgacctgcac ctcatcacca tcaccagctt
      961 caagaccggc ggcgacaccg agaagccggc catctggatc gacgccaaca tccacggcaa
     1021 cgagatccag ggtggcgagg tggcgctgta cacggcgtgg tatctcaccg agatgtacgg
     1081 ccaggtgccg gagatcaccg aactcctcga ccgcgtgacg ttctacatcg tgccgtcgat
     1141 caatcccgac gggcgggacg gcttcatcca cgagccgaac aaccgtcact cgagccggag
     1201 cggcaagcgc ccgtacgaca acgacaatga cggcctcgtg gacgaggacg gcccggacga
     1261 cctggacggc gacggcaaca tctgccagat gcggatcaag gatccgttcg gccgttacaa
     1321 ggtcgatccc gacgactcgc tgaacatggt ccccgtaaag ccaggcgaaa gcggttcgta
     1381 cacgctgctc gatgatgagg gaatcgacaa cgacggcgac ggacgcatca acgaggacgg
     1441 cctcgggggc tacgatccga atcgcaactg gccggtgaac tggcagccca cctatctcca
     1501 gaacggcgcg cacgagtatc ccggaagcct gccggaaacg cgggcgatcc tggacttcgt
     1561 ctacgcgcac ccgaacatcg gcgccgcgca gtcgtatcac aatgccggcg gcatgatcct
     1621 gcgcggcccc gggcaggaat cattgaccta cgagggtgag gacatcacgg cgttcgacac
     1681 catcggtaac gccggcgagg agatactccc cggttaccgc tacctggtca cctggaagga
     1741 cctttacccg gtgtcgggcg gcgaactcga ctggttctac ctgggacgcg gcgtgatcgc
     1801 attcaccaac gagctcttca ccgccttcaa ttacttccgc gacccggacc tccgggaaaa
     1861 cggcttcgac gacaaacccg agtcgaagtt cgacaagctc gtgttgatgc gcgaggcgag
     1921 cgtgacctgg aagccgtatg accaccccca atacggcccg atcgaggtcg gcggcacaaa
     1981 gaagaatttc ggacgcgtgc cgccgggctt cctcctcccc gaggagtgcc accggaacat
     2041 ggcgttcagc ctgtaccacg ccatgcaact gccgaagctg gtgatcggcc ggccggtggt
     2101 gacccccgtc cccggcggct tgaagcggat ccgcgtgacg gtggccaacg agtcggccat
     2161 cgccagccgc accgcgcgtg atgacaggat gaagatcacg cccgaggaca gggccacgct
     2221 gtcgggaggg ctcacgatcg tgtcgtcggg cctcctgcgc gatcgctacg gcagcgtcgt
     2281 gacgccggcg tcacgcaccg agccggccgc tgcgctccgc atcggccgcg tgccgggatt
     2341 cggcgccgtg acgatggagt ggctggtccg gggctccggc gacgcgaccg tgacgattca
     2401 gtcgacgcgc ggcggcaccg tgcggcgctc gttccgcgtg ccgtaggcgg cgccccggcg
     2461 aagccacgtc accccttcgg ccaccgaaag cccgatgagc ggcaggagcg cggctgccgt
     2521 cttccagccg tgcccggcgg atgcggcgtt gccctcggta cccgtgggtc cagccgtaac
     2581 cgacatcccc accgccgaga tcagcaccag ccccacccag aggaaccagc cgaagagccg
     2641 cgcctggcgc gattcgaagg tggcgggttc ggcgggcgtg cgggcgagga tccagtaggc
     2701 cgtgacggcg gcggcgggaa cgagcgagag gatgacgaac tcaagtgcgg gcgcaatgga
     2761 ttgaaacatg gtcgataccc ctttccccga tgatcacgcg gtcatggttg ataccagttg
     2821 acggcggatc aggtcccccc ctacagcgct atcggggtgg atagtagcgc cgccatccgc
     2881 tttgtcatct gaaatcagcg gatcgggagc gcgcgggtgc tgattccggg tgatcacccc
     2941 accggaccga ggtaacggtc gagccaggcg agaacttctt tcacgatttc ggtccgcggc
     3001 acggtgtgcc cgccggggta aatgacctgc cgcttctgcc cgggcggggc gccgagcgcc
     3061 tcgaacaggg ggcgctggct ggtgtcgagc ggaaagaaga agtcgtgttc gccgttgagc
     3121 atcaacaccg gctggcgtac gcgtccggcg tagttgacgg catcgacttc cggcaggcat
     3181 cgctggaaac agaagccggc cacgtagagc aacacggcct tgaagcgcgg ctcgtcggcc
     3241 ggaaggatgg cgcccagcgc cccaccccag ctcaagccga agtacgccag cttctccgcg
     3301 tcgatatcgt cccgggtggc cagccagtcg atcgtgcgcg aaaggtcctt ggcccacatg
     3361 atgacgtggt cacggtagtc cgcactctcg ctcggatagt cggacttgaa cccgtcacgg
     3421 cggtggtagg tggccttgta caccgggtgg atgagtgccc gcccgctctt caggatgtaa
     3481 tccgcccgcc ggatttcggc atcggtgaat tcggcggtgt ggagcgcgaa ggatcccggg
     3541 aagatgacga cggcctggtg cggcggccgg ctgttttccg gaaggtacag ataggccgtc
     3601 acccgctccc ggccgtaggc ggcgtcgaat tccacggtct gccggcggct gaacggacct
     3661 ggctcgtcgg ccacgatccg cgcgttcaag ggcgttcggt catactggaa ctgccgccgg
     3721 aagaattcaa agacggcatc cggcaccggc tcttccttcc ggaagtcgcg gaacggcatg
     3781 tcgatcgtcc gttcgaggcg cgcggcattc ggatcgactc cattccggcg gatgcaacgg
     3841 aagccgttcc cttccgaccg atcgagcgag tttcgcgtga cggcgtcggt gaacgtgtag
     3901 tccgcatcgg accaggcgcc tcccaggatg aaccgctcac ccggtcgatc cgcgtcgttg
     3961 agtgcccact cccgcacgtt ccccgccagg tcgtggacac cgaagcggtt catgctggtg
     4021 gtggcgccga ccggcatcaa tcccttgccg ccgaagttgg ccctggggac gatctgcgag
     4081 ctggccaccg tgaaggcaac ccagttccag tggaagatgg tgggaagatc gcatccgcgc
     4141 caggctgcgt aggccgccgc ctcgtaccag ctgatgccgg tgacgggatg gtcgtcctca
     4201 ccggcgggaa attcgccgaa ctcccagccg gcgggccccg gagccccgac ggtatcgacg
     4261 aatcgggcca tggccgcttc ccgggggatc ggaccgtcgg ccgtcatgaa gtcatggcgc
     4321 cagtgcgacg gctcacgata cccgcccgcc gccacgaact cgcggtagcg gcggttggtg
     4381 acgggatggc gatccatcca gaaacgatcg atcgcttccg catcgagatg atcgagtccc
     4441 ggcatgaaga gagggaactg gccggccggc acctcgacca ttccctcggg gagctcgtcc
     4501 gtggggcgca tgacgtagcg ccataggcac gcggaagggt cgctggcgcc ggacatcccg
     4561 aagtcgatgt tccagatgac gtcctcgacc ggctcgacac cgcgcgcctc gagccgcacc
     4621 cggtagattc cccggggcat gcgcgcaacg gtgatcggcg tcgtccccag gggcacccac
     4681 ggctcgccgg gagttccata gaactgcacc gtgacggccg cgcccggcgg ctcgctctcg
     4741 aaggtgaccg gattggtgaa ttccggccag agccgcgcga ggagcggatc gtccggcgtc
     4801 aaccgctcga cctcgcgcgc caggtgccac gcttcccagc atcccgatcg ctcctcgatc
     4861 cgcatggcgc cgtccacgag cgcggcgagc ttcggcaggg ccgtgagccg cacccagtcg
     4921 cccaggccgg ccagctcggc ggccaccatg ccgccatcgg ccggccgcgc cgcgggatcc
     4981 ctggccaggc agcgctcgat gagggaacgc accgcgccgg gaaggtcgtc tgccccctgg
     5041 tgcagtcgcg tcggcgctgc gtggaggatc gaaccgacga tcgagaggtt cgtgtcgccc
     5101 gcaaaggcct tgcgcccggt ggccatctcg aacaggacgc acccgagcgc gaatacgtcg
     5161 gtacgtgcgt cgacctcgcg cccctcgagc tgctccggcg ccatgtactg gagcgtgccg
     5221 acgatcaccc cctgccccgt caatggcgcg ttcacggtgg gcgactggct cgaggacgca
     5281 tccgtcacga ccccctcgag cggccgggcc aggccgaaat cgagcagctt ggggccgccg
     5341 gcggtgagca tcacgtttcc gggcttcaaa tcgcgatgga tgactcccgc ccggtgcgcc
     5401 gccgccagcg catcgacgat cggacccgcc agcgccagga attccgccgt cggaagcggc
     5461 ccccggacca gccgatcggc cagcgtctcc ccctccagga attccatgac caggtagtcg
     5521 gtgtcaccct cctgccccac gtcgaagagc gtggcgatgt tcggatggtt gagctgcgac
     5581 accgtgcgcg cctcccgctc gaagcgggcg cgcgcctcgg ccgttgcggc cagatgacgc
     5641 gagagcacct tgatcgccac cgagcggccg agccgcgtgt ccagggcgcg atacacctcg
     5701 cccatcccgc cggcgcccaa cagcgcttcc agacggtagg ggccgagtgt cgatccgggg
     5761 ggtggcatcc gggcagaata ccgtctcccg ggtgcgtggg aacatggggg cgccgaaatg
     5821 ggcaaggccc ccggccggca atggcgccga ccaggggccc gctttctgga acgtctgctt
     5881 cgctgggcag ccgcttactt cacgagcatc atccgaacga ccttcgttcc ctgggtcgac
     5941 tcggcccgga ggaagtagaa gccggccgcc gcaggcgatc cggcgtcatc acgaccattc
     6001 cactcgaagg tgtggcgacc cgcgtccatg gcgccgtcgt gcacgttgcg gacgagccgg
     6061 cccgcggcat cgaagatgcg caggtggacg tccgaagcga caggcatggc gagcaggaac
     6121 gtggtattgc cacggaacgg gttcgggtag ttctggacga gattgaacac cgcgggacgg
     6181 ccggcgtacg tgacgacgac cggtccgtag gtgacgcgac cgccgaagcg atcgaccgcc
     6241 tcgagccggt agcgatactc agcaccgtcc accacggcgg cgtcggtgaa ttcataggcg
     6301 tgccgtcccg tgatgggcga cgtgttcacc tggacgaaat cgctcgacag cccctcggcg
     6361 cgatagacgt cgaagtgcga gtgctcgacc tcctccgtcg tttcccaggc gagcttgacg
     6421 ccatccggcg tggagagcgc ctcgaagcgc gccagctcca ccgcggtggc atcctcgaca
     6481 ctgaagcaga ccgggatggt ggccttgggg agcaccgggt cgttcgtggt gatccggagg
     6541 aacccgcggt gcattccgaa cgccacaccg gtcggatcga aggccacgtt gatcaactgc
     6601 gtgcctcccg gcgggatcgt cccggacgtc ggggtggccg acagccactc ctgctcgttc
     6661 tggataagga ccgcaaggtt gttgtgcacg tagttggcgt tgaacacgac ctgcagtccg
     6721 gtcgtgcccg cggcgttctc gattccgatg gtgttgctgt tcaggatgcc cttcaccgac
     6781 aggtagttgt aggtgatgct gccgtccttg cagaggatgg cctggaacgt gagcgtgtcg
     6841 ctcagcgcgt tgaagcgcgt gaccttcttc cactccacga tgaagcggcc gagggcgacg
     6901 tcgctgcggt aatagcaggt gccgacgccg ttccgcatgt cgtagtcatc ccagagcggg
     6961 gcgatcgtgt tgttgggctg ggcggtgttc ggcagcgcgg tgttcgtgaa ggcggtggcc
     7021 gtcgaggtga aggacatcca gccgttcgaa cagacgcgca ccgagttgaa gttggtgccg
     7081 tagaacggga agttgaagcc catcgcgatc ggcgcggtgg tcacgtcgtc cgcgtggagg
     7141 ccggtgtcga tgccaccgtt cccctggagc tcgatccaca tgaactccgg accgccggcc
     7201 tggtcgctgt cgatccagcg gtagccgaag gcatccggcc caccctggcc gtacggctgg
     7261 aagccgccca ggaacggatt gcggtacccc ttggcttcgg gatcgttgcc gaccgtgccc
     7321 gcctcgtact cgggcatgac caggtccttc acttccttga agagctcgag cttcttctgg
     7381 ttgggaaccg ggtcaccgaa acggcgcgtc gacgtcgggt cgagcgcgtt cggggccgcc
     7441 ggaatcccct gggtgtcgga aaggcgccac gtgagcggca ccacgccggt gttctggatc
     7501 gacgcggccc ggtgaatgac cggccccccg aagatgccga cgaccgggca gatggaatcc
     7561 ggatcgactt ccgcacgggg cgcgcccgtc acccggaggc atatcgccac cggcacgctc
     7621 ggcgtgaccg gatcgttcga ggtgacgaag agcgtggtgt tgtacgtgcc gggcagcatg
     7681 cccgtggcgt cgaaattgag cgacaccgac gtggacgtgg caccggcgat gaccccgttg
     7741 gtggggatca ccgtaagcca cggctggtcg ggcgactcga tgatgttgaa gaccaggttg
     7801 gcataaccac acgagttggc gatgccgagc gacttgggca ggccgatgct cccggcaacc
     7861 agggtgtcgc agaccgaggt ctggttcacc gagatcgtgg gctggcacgg cgggttggag
     7921 aggcagaggt cgtccacgaa ccagtcgtcc gtggcggcct cgccggtggt gcggatgcgc
     7981 agctggaaca gcgggtagaa gtacgtcccg ctcgacggcg tgaggccgtc caggtcgatg
     8041 atgcggacgc catagttcgt ggccgcaccg gtaccgccga agacgcggtc aacctggtcc
     8101 cacacgccgc tcgagttgcg gaactccacg acgaggtcgt ttccggcctc gggcggatcg
     8161 ccgccgccgc cggcttcctg gaagtagatg agcgtcttgc cggtgccgcc tgcggtggac
     8221 aggtcgagct ggatcgacgt catcaggtcg gtgccgttca ggtcggccga ggtcggcaag
     8281 gacggctcgt tgatgccggt ggcgttcagc acgacacccg agttggtcca cctggccgca
     8341 tcgggcgcgg cgccggagtt gaagtcatcg cagaacggca atgccgcgcc gtttccggtg
     8401 acgttcagct cgacatgcac gtcggtgacc ggctcgtccg gatcgttgct ctggatcagg
     8461 atgttgccgt agtaggtgcc cggcgcgagg ccggtcggat cggacacaac gtccagcatg
     8521 gcggagtcgc ccggtggcac gactcccgag ccgggagtgg cggagagcca ggtggcgtcc
     8581 tggtagagca gcacggccag ctcgttgtgg acatacgcct ggttgaacgc aacctggagg
     8641 ccgaccgtgc cggccgcgtt ctgcaggccg accgtaccgg cgttcgaggg cgagtcgatg
     8701 tcgaggtact ggaagacgat cgtgccgtcc ttgttgatga tcacctggaa ggtgttcgac
     8761 gacaggtcat cggcgtagta cgagacggcg tcgtactgaa cgatgaaccg gttgttggcc
     8821 acgtcctggt agtagtagat cgttcccgcg ttggccgggt tgaagtcgtc ccagaacggg
     8881 gccaccaggt cggacggcgc ggcggcgttg ggcacgccgg tgttcgtgaa cggcgtggcc
     8941 gacgacgtga acgacaggaa tccgttggag cagacgcgga tccccgtgaa cgacgtgccg
     9001 tagtgattga aggcaaagcc gagggcaaag ggcccgaggt tctggtcgtc accggtgatt
     9061 ccggtgtcgg tgccgatccc cgagatgtcc acccagttga agagcgggcc gttggcttcg
     9121 tcgctgtcga tccagcggta gccggcggca tccggaccgc cctgcgcgaa aggctggggc
     9181 gggtagtgca cattctggcc gcggtcgccc aggttcttga ggcggtcacg cgaacggaca
     9241 cggtcgcccg agagctcggc ggccggatcc aatccgccgt cgatgcgggc gctgcgccgt
     9301 ccctcgtcgc cgagttcggc cgcgtaccac gccgccttca cctgggaagg tgtcggcgcc
     9361 ggggcgccgg ccatcggaag gaccgtgccg tcggcgcgct tcacgccgac gaactgcggg
     9421 ccggcccacg aacgaaccgc cgatgacgca tcgcggatga agaattccag tggaccggtg
     9481 ccgacgttgt tgatggccac cagatcgtgg gcgtccggat cggtattggc cagcgtgttg
     9541 gtgatcgagg tcgggctcac gctgatgtcg ggcgcgtcgg tcaccgtcag ggtggcggtc
     9601 accgtgatcg tcggattcac cgggtcattg gtggtcacca cgatgtcggt gttgtacacg
     9661 cccggcgcca tggccgtggc gttgaacgtg gccacgaagt tctgcgagcc gaggcccgag
     9721 atgctcccgc tggcgccggt gagcaccagc cacgaaacgt cggggctctc ggcggcggac
     9781 caggtgaggg cattgaactg gcagctgttc gacacggtca cgacgtcggc atcacctgtc
     9841 ggatcgccgg ccaccagcgt gtcatccacg ctggtcgggg ccaccgtggc ggtggcctgg
     9901 cacgacggga tgtcgagcgt ggtgcagtcg acgaaccagt tgtcgaaccc gggatcgccg
     9961 gtggagcgga agcggaaccg gaactgcgag tggaagtagg tgccgctcga cggaacaagg
    10021 ctgtcgagcg cgaggacgtg ggtcgtgaac gacacttcgt cgccatcgac gcccaggtgg
    10081 cggttcactt catcccatac accgttcgag ttgtagagct cgatgacgag gtcgttggtt
    10141 ggatcgggcg attcgccgtt accggtctgc tccttctggt agagcagcac gtaaccggaa
    10201 cccgatgccg cgcccatgtc gatcgcccgg ctggtcacga cgtcctggcc gtccaggtcg
    10261 agcgagttgg ggggcgtggg cgggttcgag ccgaggccgt ccacgaccgc accatcggtg
    10321 gtccacttgc cggggtcggg cgcgccggcc acgtcaaagc agtcggaaaa cggcggattg
    10381 agcggcacct ccacgcagat ctcggtggtg aaggcgctct cgtgcagcgg gatctcggag
    10441 tcgatcgccg tcagtttgta gcagttgagc ccgggtacgg gcgccaggtc ggaataggtg
    10501 ccgggaaggc cgatgtcacc cggggcgaac gagacggtcg tcaggtaggc gccgttccgg
    10561 tagacgttca cgccgtcgaa gtcacagagc tggctcccgt cgatgttgac cgtggggttc
    10621 gtccagtcga ggtcgaacgc cggaccggga atggccccga cggtgaagag cgtgggcggg
    10681 gccggcgtcg gatcgccgcc cgcgtacttg gtgatggtgg ccacgatcga gaggctgtcg
    10741 ttgtcctcgt cgtgggtgcg cagctcgtag gtgtagaggg tgcccgtcgt gaggccggca
    10801 tccgtgtaag tttcgatccc ctggtccacg tccaccgtgg ggccgccgag gcgggtgatc
    10861 cggatctcga agttccccag cggcgtgccg ttcacgcgcg tggtcgggtc gtcccagtcg
    10921 agcgtgacgc tggtcggcgt cgtgcagtcg ctggtggcga tgatgttgct cggggcgttc
    10981 ggatcatcgg gatccgcgta cacgatctcg tcccagtacg cattgtactg gctggtccgg
    11041 ttgtcggccc acgccgcgaa ggcgcggccg ttataggcgg cgatctggta atagtcgccc
    11101 gcgtagccgg cggcgaacga gccacccagc gccgtcggga tggtgtagac gtcctgcacc
    11161 gggaacgaat cgaaggtgtt gccgccgt
//
LOCUS       DASVOD010000279        11808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_227963, whole genome shotgun sequence.
ACCESSION   DASVOD010000279 DASVOD010000000
VERSION     DASVOD010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227963"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="VF720_06100"
     CDS             complement(<1..1569)
                     /locus_tag="VF720_06100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878962.1"
                     /translation="MATGTRAPEAPGRPPAQPPSVHEPTTPARRLDSSAAATIDSGST
                     LSSSSGHHGRFDPGTRLGTRYRIVALLGKGGMGEVYRADDLELGQSVALKFLPPGVAA
                     NAAELTRFRNEVRTARLIAHPNVCRVYDIGEADGHVFLSMEYIDGEDLASVLRRMGRP
                     TEDKALEIARQICLGLAAAHEAGMLHRDLKPANVMIDGRGRARITDFGLAGLADDLAR
                     GGQFAGTPSYMAPEQLSGGRVSVKSDVYALGLVLYELFTGKRAIQAGSMAELRQLHES
                     GSITTPTEVARSIDPAVERVILHCLERDPESRPPSAYAVLGALPGGDPLAAAMAAGET
                     PSPELVANASDRGTLSPLVAILLVLAGLLGLGTWAGVIGPEFRAMTQPTPVLAVRAAD
                     LLSKTGSFETLPKYSAEGFTLNDAYLEHLKKARSRQAPRRSAMYFWRRWSPELVESAN
                     LHSEIATIYDPRRLATGEAVVLFDPAGRLIGLRAVPPDSVDAAHDGRPRWSTLWEAAG
                     LDSATFKPAPLARPS"
     gene            complement(1808..2470)
                     /locus_tag="VF720_06105"
     CDS             complement(1808..2470)
                     /locus_tag="VF720_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEX7878963.1"
                     /translation="MNRSVQHLLMSLWTAEREGESRDPVLHEQARLTDAAERHLRREA
                     VEAGYLVQHAAGWRLTNEGEREARSLLRRHRLAERLLSDILELPEEEFEASACRFEHH
                     LTETVTERICTLLGHPTTCPHGRTIPPGDCCHRRARTLEPILSRLSDLAPGERGRVAF
                     LTPTGSGASSRLGALGVTPGAVIRLLRKGDCFLVEAGATELAFDGRAGDDVFVSRMPD
                     LR"
     gene            complement(2670..4616)
                     /gene="feoB"
                     /locus_tag="VF720_06110"
     CDS             complement(2670..4616)
                     /gene="feoB"
                     /locus_tag="VF720_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEX7878964.1"
                     /translation="MESTRSLRRVVLVGNPNVGKSLIFSCLTRAYATVSNYPGTTVEV
                     TRAAGRAIPREPEVIDTPGTNSLVPLSDDERVTRDLLLEPFDTTVILVGDAKNPRRTL
                     LLALELAELDLPFLVVLNMADEADERGIPCDVPLLSAWLGVPVIRTVATRREGIDALI
                     AALPEARTSARRAALPPDLEEARAEVVRLLPTTMRGRDAVAGMVLSGDFELLGRLVPL
                     IGLDAAARIQSGARRLSVDFPASLAGRITRARLKPVDRFLTAATPHHHLARSLRRTLG
                     DLAVHRFWGIPIVLMVLWVSYEVVGVFGAGIAVDWLETHVFGGYVNPWVTGLAGHLPW
                     PILRDLLVGEYGIVTMALTYAIAIVLPIVFFFFVIFGMLEDSGYLPRLAVMLNRPMRV
                     IGLNGKAVLPMVLGLGCDTMATLTARVLETKRERLLVTFLLALAVPCSAQLAVVLALT
                     RRLSPMITLVWAGVVLASLVGVGWLAARLVPGDRSDFILELPPLRRPRLANILNKTLA
                     RLEWYLKEAVPLFVAGTLLLFVLDRIGALVVLQKAAAPVVVNLLSLPPQMASAFVVGF
                     LRRDYGSVYLAEAAGRGELSGVQVLVSLVVLTLFIPCVANYLIMVKERGFRTAVAMAA
                     FIVPFAFLMGAALNWALHLAGITL"
     gene            4742..6340
                     /locus_tag="VF720_06115"
     CDS             4742..6340
                     /locus_tag="VF720_06115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7878965.1"
                     /translation="MARQELIGRFKTGPTPIVRHVSTSSAPGLPDDILREAGNRLAIM
                     GLVAAALWFIGTTAGHLVEREMTGGAPGWMELGASDYVSLLAIGVSLFVFWYARRPGR
                     STAHLVHLGPLFLIFTAFTLALMTHLDELPAHWMFHPMITWTGVSILTFAAIVPSPPW
                     TTALASFAAASMNPISLMLARFRGGSWERVTLSDVVVSYHCDYLLAGVAVVISLVITR
                     LGQEVTKAREMGSYQIGELIGAGGMGEVYRATHRMLARTAAIKLIRPEVLGASDTHSA
                     EMAVARFKREAQVAANLRSPHTIELYDFGVTEDRTFYFVMEFLEGSNLEALVARRGPL
                     PANRTVHILRQVCESLEEAHAAGLVHRDIKPANIHVGKLGVRHDFVKVLDFGIVKSVR
                     ESDGTKTMETAAGFLPGTPAYMAPELAIGDSYDGRADIYAVGCVAFFLLTGKLVFEGS
                     NSLDMMMKHQSAQPERPSRKSPELVPAALDALILACLEKNPANRPQSAVELSRGLAAI
                     QLPTWTEENARSWWEEDERTTRSA"
     gene            6361..6852
                     /locus_tag="VF720_06120"
     CDS             6361..6852
                     /locus_tag="VF720_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006914716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7878966.1"
                     /translation="MSITPFVPADFKVPERLETDEFRLRMLTVHDVVKDYDAVMSSLD
                     HLKTIWPGGKWPVGLTLEQNLIDLGWHQKEFQTRRSFAYTVVAPDESRVVGCVYIDPT
                     QKLAYDAVVYLWARQGELASGLEGRLHAAVREWMARAWPFGRVGFPGRDIAWEEWRAI
                     PEE"
     gene            complement(6893..7438)
                     /locus_tag="VF720_06125"
     CDS             complement(6893..7438)
                     /locus_tag="VF720_06125"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7878967.1"
                     /translation="MFVKVLIGIAALVVLVLIVAATRPTAYHVERTLEIAAPTNVVFG
                     ILNDLHQFPGVMVIFGSPFETSDATMQKTIEGPASGPGQSYAWVGKDAGKGRMTIEKS
                     IPDQQVVTRLDFEKPMKSTSITTLALAGTPTGSQVTWTMDGNHNLIGKVFGLFMNMDR
                     MLGADIEKSLERLNTVAAGKR"
     gene            7703..8146
                     /locus_tag="VF720_06130"
     CDS             7703..8146
                     /locus_tag="VF720_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7878968.1"
                     /translation="MPGTVRFHRVLKATPERIYRAFLDPDAMSKWLPPNGFTGHVHHL
                     EAKVGGTYKMSFTNFTSGKSHSFGGSYLELVPDQRIRHTDTFDDPNLPGEMVVTIDLK
                     PVSVGTEVNIVQEGIPDVIPTEACVLGWQESLALLAMLVEAAIPD"
     gene            8146..8697
                     /locus_tag="VF720_06135"
     CDS             8146..8697
                     /locus_tag="VF720_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7878969.1"
                     /translation="MAPITTPSLILRPFTPDDTPDMWRLSQEEAYRLWLSNQVYGDPA
                     EMASVMSYLIAQYESPGDPRQGPFVFAIEYAPDRTLIGHVGFSRLDDEVEVGFSIGEA
                     HQRRGLATEAVAAASSQVLRALGLERIIGVTAVANTASRRTLVRAGYSWERDREMNFQ
                     GVVQTVNVYVYRPLRVSHTPSDS"
     gene            8694..9110
                     /locus_tag="VF720_06140"
     CDS             8694..9110
                     /locus_tag="VF720_06140"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7878970.1"
                     /translation="MNSIELLRDNLVRSRERVLSRVEEMRDHAIVFPTLNGGGHTLWV
                     LGHLAYIEALIRHRFVLGEANPLAGWEGVFDGADVSGDPLLYPPFDDVLARCRAERES
                     TLALLAGLSEDDLDRTSGFVPPGFESTFGTWRSCLQ"
     gene            9383..10348
                     /locus_tag="VF720_06145"
     CDS             9383..10348
                     /locus_tag="VF720_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX7878971.1"
                     /translation="MPLDPIRPISLDEILAARERIAGTIVRTPLLRLEQGGRRPDIWL
                     KLENLQPINAYKLRGAANAVAMLSAEERSRGVWTISAGNAGQGVAYAARQAGVGCTVV
                     AIETAPTAKLDRMRALGARLVLVPFNDAWQAMEDRAYPGVEGTFIHPFDDDHFVAGHG
                     SMGLEILEDLPEVAVVVASVGGGGLITGVGTAIKARRPSVRVIGVEPETASPMAHSLA
                     AGFPQEFPNWQATFVDGAGGRGVFPRMWERMRPVIDDSVIVTLEETRRAMKTMAETCR
                     VIAEGAGALPLAAALAGKVGNEGPIVAVVSGGNIDLPKFFELIAG"
     gene            complement(10382..10609)
                     /locus_tag="VF720_06150"
     CDS             complement(10382..10609)
                     /locus_tag="VF720_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX7878972.1"
                     /translation="MKTTITIRIDTVTKHLIEDLAWDTGRTTSEVVRQAIDEFVMPLT
                     FRRMKTPQDRDAWLARRAANNWRPRMGRKSG"
     gene            10825..11256
                     /locus_tag="VF720_06155"
     CDS             10825..11256
                     /locus_tag="VF720_06155"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX7878973.1"
                     /translation="MAISKSPTKRKAAAPASKASNRSSSRHADALAEFCRNLPGATED
                     VKWGKDLIFSVGGKMFAGFQLPEGEPLGFKVDPPTFDDWVKRPGVIPAPYMARHSWIS
                     VTDRTKVAESTLKKLLKESHRLTAEKLSGKMREKLGLSGDS"
     gene            11310..11801
                     /locus_tag="VF720_06160"
     CDS             11310..11801
                     /locus_tag="VF720_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7878974.1"
                     /translation="MRRLLALTAVTVLALVLATTPLHADSKKSPPKDVGFGRIAWFDL
                     VTTDIAKTKEFYSKLLDWKYTAVEGTDLAVEIVSRDLPIGTIRSADGTPSPYNGVVYV
                     QVDDIEQSTARAKELGGTVPPGFPFNLPDGIGAISLLVDPGGQPIGLYSRKLIPVKDK
                     KEK"
BASE COUNT         2026 a         3718 c         4107 g         1957 t
ORIGIN      
        1 cgagggccgg gccaggggcg ccggcttgaa cgtggcggag tcgaggcccg ccgcctccca
       61 cagcgttgac caccggggtc gtccgtcgtg agccgcatcc accgagtcgg gcggcacggc
      121 gcgcaggccg atgagccggc cggcgggatc gaacaggacc acggcctcgc ccgtggcaag
      181 gcgcctgggg tcatagatgg tggcgatctc cgagtggagg ttcgccgatt cgaccagctc
      241 ggggctccag cggcgccaga aatacatcgc gctccgtctt ggcgcctgcc tggacctggc
      301 tttcttgagg tgctccagat aggcgtcgtt gagcgtgaag ccctcggccg agtacttggg
      361 caacgtttcg aaactgccgg tcttcgagag gagatcggcc gcgcgcacgg cgagcaccgg
      421 cgtgggctgt gtcatggccc ggaactcggg gccgatgacg ccggcccagg taccgagccc
      481 gagcaggccg gccaaaacga gcaggatggc caccagcggt gaaagcgtgc cgcgatcact
      541 ggcgttggcc accagctcgg gcgacggcgt ctcgccggcc gccatggccg cggccagggg
      601 atcgccgccg ggcaacgcgc cgagtaccgc gtaggccgat ggcggtcgcg actcgggatc
      661 gcgctccagg caatggagga tgacgcgctc gacggccggg tcgatgctcc gcgccacctc
      721 ggtcggcgtg gtgatcgagc ccgactcgtg aagctggcgc aactcggcca tgctgccggc
      781 ctggatggcg cgcttgccgg tgaacagttc gtacaggacg aggccgagcg cgtacacgtc
      841 gctcttgacc gacacgcgcc cgcccgacaa ctgttccggc gccatgtacg agggcgtgcc
      901 ggcgaattgg ccccccctgg ccaggtcatc cgccagtccg gccaggccga agtcggtgat
      961 gcgcgcacgg ccgcggccgt cgatcatcac gttggccggc ttcaggtcgc ggtggagcat
     1021 gccggcttcg tgggccgccg cgaggccgag gcatatctgt cgggcgatct cgagcgcctt
     1081 gtcttcggtc ggccggccca tgcgacggag caccgaggcc aggtcttctc cgtcgatgta
     1141 ttccatggag aggaacacgt ggccgtcggc ctcgccgatg tcgtagacgc gacagacgtt
     1201 gggatgcgcg atcaaccgcg cggtgcgcac ctcgttgcgg aagcgggtca gctcggcggc
     1261 attcgcggcc acgccaggcg gcaggaactt gagcgccacc gactggccga gctccaggtc
     1321 atccgcccgg tacacctcgc ccatgccgcc ctttccgagc agcgccacga tgcggtaacg
     1381 cgtgccgagg cgcgtgccgg ggtcgaaacg gccgtggtga cccgaggacg atgacagcgt
     1441 ggagccggag tcgatggttg cggctgccga ggagtcgagt cggcgggcgg gagtcgtggg
     1501 ctcatggacg gagggaggct gcgctggtgg acgcccgggg gcctcgggtg cccgtgtgcc
     1561 cgtggccacc ggggcaccgc aggccgcgca gaatctggct ccgtcaggga tcgactgagc
     1621 acattgggtg cagtagggca tcaggcgtgc ggatctccgg cgggattccg tcgcggctcg
     1681 gaacgcagca tggcgggcat cctaggggag atgggggcgc gtgaacaggg tgcgggggcg
     1741 gggatgatga ggactggcgg acactgcggg cgaccatcga cgagttcctc tgagatcccc
     1801 gccgcgttta ccgaagatcc ggcatccggc tgacgaacac gtcgtccccg gcccgcccgt
     1861 cgaaggccag ctccgtggcc ccggcctcga ccaggaagca atcccctttc ctgagcagcc
     1921 ggatcacggc tcccggcgtc acgcccagcg cgccgagacg tgagcttgct ccgctgccgg
     1981 tcggcgtgag gaacgccacg cgtccccgct cgcccggggc gaggtcggag agcctcgaca
     2041 gaatcggctc cagcgtgcgg gcgcgccggt gacagcagtc accgggcggg atcgtccggc
     2101 cgtgcggaca ggtggtcggg tggccgagca gcgtgcagat gcgttcggtc acggtctcgg
     2161 tgaggtgatg ctcgaagcgg caggcgctcg cctcgaattc ctcctcgggg agctccagga
     2221 tgtcggaaag gagccgctcg gccaggcgat ggcggcgcag cagcgaccgc gcctcccgct
     2281 caccttcatt ggtcaggcgc cagccggccg cgtgctgaac caggtagccc gcttccacgg
     2341 cctcccgccg gagatgccgt tcggccgcgt cggtgagcct ggcctgttca tggagaaccg
     2401 gatcgcgtga ttccccctcg cgctcggcgg tccacaggct catcaggaga tgctgaacgg
     2461 atcgattcat ggggtacctc gtccggcgcc ggaaggacgc cggtcatgtg agggaaagaa
     2521 ccggcgaagg acccggacga tggcggagtc acccaccgtc tggtagccgc aggccggaca
     2581 gcagatcgtg cgacaggacg gcgaaagggg gcagcgggag cagccggcgt tggccgcctc
     2641 atcgaagcga tgcccgcaga gcgcgcaggt cacagcgtga tccccgcgag atggagtgcc
     2701 cagttgaggg ccgcgcccat gagaaacgcg aacggcacga tgaacgccgc catggcgacg
     2761 gccgtgcgga atccgcgctc cttgaccatg atcaggtaat tggccacgca cggaatgaac
     2821 aacgtcagga cgaccagtga cacgaggacc tggacgccgc tcagctcgcc ccgtcccgca
     2881 gcctcggcca ggtagaccga gccgtagtcg cgccgaagaa acccgacgac gaaagccgag
     2941 gccatctgcg gcggcaggga gaggaggttg acgacgaccg gagcggccgc cttctgaagc
     3001 accaccaggg cgccgatccg atcgagcacg aacaggagga gagtgccggc cacgaagagc
     3061 ggcaccgctt ccttcaggta ccactcgagc cgcgccagcg tcttgttgag gatgttcgcc
     3121 agccgcggcc ggcggagcgg cggcagctcg aggatgaagt cgctgcgatc gcccgggacg
     3181 agccgtgcgg ccagccagcc gacgccgacg agcgaggcca gcacgacacc ggcccacacc
     3241 agtgtgatca tcggcgacag tcgccgggtg agcgccagga caacggccag ctgtgccgag
     3301 caggggacgg ccagcgccag gaggaacgtg accaggaggc gttcgcgctt tgtctcgagc
     3361 acgcgcgcgg tgagcgtggc catcgtgtcg cacccgaggc cgagcaccat cggcagcacg
     3421 gctttgccgt tcaggccgat gacacgcatc gggcggttca gcatcacggc caggcgcggc
     3481 aggtagcccg agtcttccag catcccgaag atgacgaaga agaagaagac gatgggaagc
     3541 acgatggcga tcgcgtacgt gagcgccatg gtgacgatgc cgtactcgcc gaccaggagg
     3601 tcccggagga tgggccacgg caggtgcccc gcgaggcccg tgacccacgg gttcacgtac
     3661 cccccgaaga cgtgcgtttc gagccagtcg acggcgattc ccgctccgaa aacccccacg
     3721 acctcgtagc tcacccagag gaccatgagg acgatcggaa ttccccagaa gcgatgcacc
     3781 gcgagatccc cgagggtgcg tcgcaatgat ctcgcgaggt gatgatgcgg cgtggccgcc
     3841 gtcaggaacc ggtcgaccgg cttcagtcgc gcccgcgtga tgcgaccggc cagggaggcg
     3901 ggaaagtcga ctgacaaccg gcgcgcccct gactggatcc gggccgcggc atcgagaccg
     3961 atgaggggga ccagccggcc gaggagctcg aaatctcccg acagcaccat gcccgccacg
     4021 gcatcgcgac cgcgcatcgt tgtcgggagg aggcgcacga cctccgcgcg tgcctcctcg
     4081 agatcgggcg gcagggccgc ccgacgggcg ctggtccggg cttcgggcag ggccgcgatc
     4141 agggcgtcga ttccctcccg ccgcgtcgcg acggtgcgga tcaccggcac gcccagccac
     4201 gccgacagga gggggacatc gcacggaatc ccgcgctcgt ccgcctcgtc ggccatgttg
     4261 aggacgacga ggaacggaag gtcgagctcg gccagctcga gggccagcag cagcgtgcgg
     4321 cgcgggttct tggcgtcgcc caccaggatc acggtcgtgt cgaacggctc gagcaggaga
     4381 tcccgcgtga cgcgctcatc gtccgagagc ggaacgagcg aattggtccc cggcgtgtcg
     4441 atgacctcgg gttcacgcgg aatggcgcgc cccgcggccc gggttacttc gacggtcgtg
     4501 ccggggtaat tggagaccgt ggcatacgcc cgggtgagac aggagaaaat gaggctcttg
     4561 ccgacgttcg ggtttccgac caggacaact cggcggagtg accgggtcga ttccaccggg
     4621 tattccatga gcaccgacga catgagggga tgttaggcat acccgacagg catcaggatg
     4681 acaattgtca gccccgatcg gtgaatgaac cccgctgtgc taccttgcca cggggggatc
     4741 gatggccaga caggaactga tcggccgctt caagaccggg cccacaccca tcgtacgtca
     4801 cgtctccacg agttccgctc cgggccttcc ggacgacatt cttcgcgagg cgggaaaccg
     4861 gcttgcgatc atggggctgg tggcggcggc gctctggttc atcggtacga cggcgggtca
     4921 ccttgtcgag cgggagatga ccggcggggc tcccggatgg atggagctcg gcgccagtga
     4981 ttacgtttcg ctgctggcca tcggggtttc gctcttcgtc ttctggtatg cgcgccgtcc
     5041 ggggcggtcc accgcgcatc tcgttcacct cggccccctc ttcctgatct tcaccgcgtt
     5101 cacgctcgca ctgatgacac acctcgacga gttgccggcc cactggatgt ttcaccccat
     5161 gatcacctgg acgggcgtct cgatcctcac tttcgcggcg atcgtcccga gccctccctg
     5221 gaccacggcc ctcgcttcgt tcgcggcggc ttcgatgaac ccgatttccc tcatgctggc
     5281 caggttccgc ggaggaagct gggagcgggt cactctgagc gacgtggtcg tgtcgtacca
     5341 ctgcgactac ctgcttgccg gtgtcgcggt ggtcatctca ctggtgatca cgcgcctcgg
     5401 gcaggaggtg acgaaggccc gggagatggg gagttaccag atcggcgagc tgatcggcgc
     5461 cggaggcatg ggcgaggttt atcgcgccac ccaccggatg ctggcgcgga cggcggccat
     5521 caagctgatc cgtccggaag tgctcggcgc ttcggatacg cattcggccg agatggccgt
     5581 ggcccgcttc aagcgcgagg cgcaggtggc ggccaatctt cgctcgccgc acacgatcga
     5641 gctgtatgac ttcggtgtca ccgaagaccg cacgttctac ttcgtcatgg agttcctcga
     5701 gggatcgaac ctcgaggcgc ttgtggcgcg gcgcggtccg cttcccgcga accgcacggt
     5761 gcacatcctc cggcaagtct gcgaatcgct cgaggaagcg cacgcggcgg ggctggtgca
     5821 tcgggacatc aagccggcga acattcacgt cggaaagctc ggcgtccgtc acgacttcgt
     5881 caaggtgctc gacttcggca tcgtgaaatc cgttcgcgag agcgacggga cgaagacaat
     5941 ggagacggct gccggtttcc tgccgggaac gccggcctac atggcgcccg aactcgcaat
     6001 cggggactcc tacgacggcc gcgccgacat ttacgcggtc ggttgcgtcg ccttttttct
     6061 tctcacgggc aagctcgtct tcgaaggctc gaactcgctc gacatgatga tgaagcacca
     6121 gtccgcccag ccggagcgtc cgtcgcgaaa gagccccgag ctcgtcccgg ccgcgctgga
     6181 cgccctcatt ctggcctgcc ttgagaagaa cccggcgaat cgaccgcaga gcgccgtgga
     6241 gctgtcgcgc ggcctcgcgg cgatacaact cccgacctgg accgaggaga atgcgcgaag
     6301 ctggtgggag gaagacgagc gaaccacccg atccgcgtag ccgttgcagg agatcaccaa
     6361 gtgtcgatca ccccattcgt cccagccgac ttcaaggttc ccgaaaggct cgagacagac
     6421 gaattccgcc ttcgcatgct gacggtccat gacgtggtca aggactacga cgccgtcatg
     6481 agcagcctag accatctcaa gacgatctgg ccgggcggca agtggccggt gggcctgaca
     6541 ctcgagcaga acctcatcga tctcggctgg caccagaagg agttccagac gcgccggtcg
     6601 ttcgcgtaca cggtggtggc tcccgatgag tcccgggtcg ttggttgcgt gtacatcgat
     6661 ccgacgcaga agctggcgta tgacgcggtg gtctacctgt gggcccgtca gggcgaactg
     6721 gcctcggggc tcgaggggcg cctgcatgcg gcggtccggg agtggatggc gcgcgcgtgg
     6781 ccgttcgggc gcgtcggatt tcccggaagg gacattgcct gggaggagtg gcgggcgatt
     6841 ccggaggagt agcggcaggc accggcactc cgtcgcgccg gcccggcttc ggctatcgct
     6901 tccccgccgc cacggtgttc aggcgctcga ggctcttctc gatgtcggcg ccgagcatcc
     6961 tgtccatgtt catgaacagg ccgaagacct tgccgatcag gttgtgattg ccgtccatcg
     7021 tccaggtcac ctgcgagccg gtgggcgtgc cggccagggc gagcgtggta atcgaggtgg
     7081 atttcatcgg cttctcgaag tcgagcctgg tcacgacctg ctgatcggga atgctctttt
     7141 cgatcgtcat cctgcccttg ccggcgtcct tgcccaccca tgcatacgac tgaccggggc
     7201 cgctcgcggg gccctcgatg gttttctgca tcgtggcgtc cgacgtctcg aagggtgatc
     7261 cgaagatgac catgacgccg gggaactggt gcaggtcatt gaggattccg aacacgacat
     7321 tggtcggagc ggcgatttcg agcgtccgct cgacgtgata ggcggtggga cgcgtggcgg
     7381 ccacgatcaa cacgagaacg acgagcgccg caatgccgat gagaaccttc acgaacatgg
     7441 aagccctccg ggatggggga tggctcgtgg acgggcggaa acgagccggc agtatacccc
     7501 cggaggaacg gttcgtgatg gcggctgcct ggtttgtgtc gtgccggtcg ggcctttcgg
     7561 catttcttcc cggcgtcgcc cggcgcgggt agactggttc aggacaacct ggtctcgaaa
     7621 gggtgcacgt cgccgttcgc gccgatccgg atcgatttca tggtgaagcg gcaaggatcc
     7681 tgatatccca aaggagcaag tcatgcccgg caccgtccga tttcaccgcg tactgaaggc
     7741 cacgcccgag cgcatctatc gggcgttcct cgatcccgac gccatgtcca agtggctgcc
     7801 gccgaacgga ttcaccggcc acgtgcacca cctcgaggcc aaggtcggcg gcacgtacaa
     7861 gatgtcgttc accaatttca ccagcgggaa gagccattcg ttcggcggct cgtacctcga
     7921 gctcgtgccg gaccagcgga ttcgtcacac cgacacgttc gacgacccga acctccccgg
     7981 cgagatggtc gtgaccatcg acctgaagcc ggtttccgtg gggacggaag tgaacatcgt
     8041 gcaggagggg attcccgacg tgatcccgac cgaggcgtgc gtgctggggt ggcaggaatc
     8101 gttggctctg ctggcgatgc tggtggaggc ggcgataccg gattgatggc gcccatcacg
     8161 acgccttcgc tgatcctgcg tccttttacg ccggacgaca ctcccgacat gtggcggttg
     8221 agccaggaag aggcgtatcg cctgtggctc tcgaaccagg tgtatggaga tccggccgag
     8281 atggcgtcgg tgatgtcgta cctgatcgcg cagtacgaaa gtcccggtga tccccggcag
     8341 gggccgtttg tctttgcgat cgagtacgca cctgaccgga cgttgatcgg ccacgtgggg
     8401 ttcagtcggc tggacgacga ggtcgaggtc ggcttctcga tcggcgaagc gcatcagcgg
     8461 cgcgggctgg ccaccgaagc ggttgcagcg gcctcgagcc aggtcctgcg cgcactcggc
     8521 ctggaacgga tcatcggcgt taccgcggtg gccaataccg cgtcaaggcg aaccctggtg
     8581 cgcgccggct attcctggga gcgcgaccgc gagatgaatt tccagggggt ggtccagacc
     8641 gtcaacgtgt acgtgtaccg acccttgcgc gtgagccaca ccccaagcga ctcatgaact
     8701 cgatcgagct gttgcgggac aacctggtcc ggagccgtga acgcgtcctc tcccgcgtgg
     8761 aggagatgcg ggatcatgcg atcgttttcc cgacgttgaa cggtggaggc cacacgctct
     8821 gggtgctggg tcacctggcg tacatcgaag ccttgattcg gcaccgattc gtcctcgggg
     8881 aggccaatcc cctggccggg tgggaggggg tgttcgacgg tgccgatgtg agcggggatc
     8941 ccctgcttta tccacccttc gatgacgtgc tggcacgctg ccgcgcggag cgggaatcga
     9001 cgctggcgct ccttgccggg ttgtcggagg acgacctgga tcggaccagc gggttcgtgc
     9061 cacccggctt cgagtcgacg ttcggaacgt ggcggtcatg cctgcagtaa gcggctgacc
     9121 actggtacat gcatcgaggg caactcgcgg atgcgcgccg ggcggcgggg atggggcgga
     9181 tctgggtgtg acgggaccgg tggccggagt gcgggtgccc ccggccgttc cgagaggggc
     9241 gtgccgttgg ttgtcccgcg cggtgtctta caatgtaaga cagtgcgtga aaggatgacc
     9301 gaagccccgg cgaatcgcgc cccctcgcga tgcggaatcc gccacgcggt ccctgccccg
     9361 aacccgtcac cgtggagact ccatgccgct cgatccgatt cgaccgatct ccctggatga
     9421 aattctggct gcccgcgagc gcattgcggg caccatcgtg cggacaccgc tccttcgcct
     9481 cgaacagggc gggcggcggc ccgacatctg gttgaagctc gagaatctcc agccgatcaa
     9541 cgcctacaaa ttgcgcggag cggccaatgc ggttgccatg ctgtcggcgg aggagcgctc
     9601 ccgcggcgtg tggacgatca gcgcggggaa tgccggccag ggcgtggcct atgccgcgag
     9661 gcaggcgggc gtcgggtgca ccgtggtggc catcgagacg gcgccgacgg ccaaactcga
     9721 ccggatgcgc gcgctcgggg ccaggctggt gctcgtcccg ttcaatgatg cctggcaagc
     9781 catggaagat cgcgcctacc ccggcgtgga gggaacgttc atccacccgt tcgacgatga
     9841 ccatttcgtg gcaggccacg gatcgatggg tctggaaatt ctcgaagacc tgccggaagt
     9901 ggcggtggtc gtggcgtcgg tcggcggtgg cgggctcatc accggtgtgg gaacggcgat
     9961 caaggccagg cgtccttccg tccgcgtgat cggcgtcgag cccgagacgg cctcgccgat
    10021 ggcgcattcc cttgccgccg gatttcccca ggagttcccg aactggcaag ctaccttcgt
    10081 cgatggggcg ggtggccggg gggtgtttcc gaggatgtgg gagcggatgc gcccggtcat
    10141 cgacgattcc gtgatcgtga ccctcgagga aacacgccgg gccatgaaga cgatggccga
    10201 gacctgccgc gtcattgccg aaggggccgg cgcgctgccg ctggcggcgg cgctggccgg
    10261 aaaggtgggg aacgaaggcc ccatcgttgc ggtagtgtcg ggtggcaaca ttgatctgcc
    10321 gaagttcttc gagttgatcg ccgggtgacg attcacggac ctccccgccg ctttcggaag
    10381 gctagcccga cttcctgccc attcgtgggc gccagttgtt cgcggcgcgc cgggccagcc
    10441 aggcatccct gtcctgcggc gttttcatgc ggcggaacgt cagtggcatg acgaattcat
    10501 caattgcctg gcggacgacc tcgctggtcg tgcgcccggt gtcccaagcc aggtcctcga
    10561 tgaggtgctt cgtgaccgtg tcgattcgga tggtgatcgt cgttttcatg cattggttct
    10621 acgagagatg cttttgagcc gtcaacgggt gaaattgtga ttcgtgcaat tcggggcggc
    10681 gcgtggttgg tgtaaatcca acgtgtctta caatgtaaga cagtgcacga ggacactgga
    10741 cacggcccgg tccgggaccg tttccgcaag ccgacgcgcg cttcacctgt catcaataca
    10801 gttttccgaa caacgggaga gttcgtggcc atcagcaaga gtccgacgaa acgaaaggcg
    10861 gcagcccccg cctcgaaagc cagcaacaga tcgtcgtccc ggcacgcgga cgcactcgcc
    10921 gagttctgtc ggaacctccc cggcgccacc gaggacgtga agtggggaaa ggatcttatc
    10981 ttctcagtcg gcggaaagat gttcgcgggg tttcagctgc cggagggtga gccgttggga
    11041 ttcaaggtgg acccgccgac attcgacgac tgggtcaagc ggccgggcgt gatcccggcc
    11101 ccgtacatgg ccaggcactc gtggatcagc gtgaccgatc gcacgaaggt ggccgagagc
    11161 acgctgaaaa agctgctcaa ggaatcgcac cgactgacgg cggaaaagct ctcggggaaa
    11221 atgcgggaga agctggggtt gtcgggcgat tcgtgagcag ggacgtgatg tacagtaggg
    11281 cgtccaccca ccctttcctg gaggttcgca tgcgccgtct cctggcactg actgccgtca
    11341 ccgtcctcgc cctcgtcctc gccaccacgc cgcttcacgc cgactcgaag aagtctcctc
    11401 ccaaggacgt gggtttcggt cgcattgcct ggttcgacct ggtgacgacc gatatcgcca
    11461 agaccaagga gttctactcg aagctcctgg actggaagta caccgcggtg gaaggcaccg
    11521 accttgcggt ggaaatcgtc tcgcgggacc tgccgatcgg aacgattcga tcggccgatg
    11581 gcacgccgag tccctacaac ggggtcgtct acgtgcaggt ggatgacatc gagcagagca
    11641 cggccagggc gaaggaactg ggaggtacgg tcccaccagg gtttccgttc aatttgcccg
    11701 atggtatcgg cgccatctcg ctcctcgtcg atccgggcgg acagccgatc gggctctact
    11761 cgcgcaagct gatcccggtg aaggacaaga aggagaagta gggtcgtg
//
LOCUS       DASVOD010000280         9771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_539906, whole genome shotgun sequence.
ACCESSION   DASVOD010000280 DASVOD010000000
VERSION     DASVOD010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9771
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539906"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF720_06165"
     CDS             complement(<1..284)
                     /locus_tag="VF720_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008583710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7878975.1"
                     /translation="MPGRRDYYDVLGVARDADVDAIKKAYRKLAMQYHPDRNPGDAAA
                     EESFKEATEAWEVLHDPQKRAAYDRFGHDGLKGAGFGAGHPFDLQDALRA"
     gene            complement(284..889)
                     /locus_tag="VF720_06170"
     CDS             complement(284..889)
                     /locus_tag="VF720_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX7878976.1"
                     /translation="MSTHFNDGRTPADNADDREFEAGPDGDPGFTDDPESEGAGASSR
                     VEAGPGAAHAGADGVAADDRYLRLLAEFDNFRRRSHRDREQAVQLGIERLASPLFDVL
                     DNLERALAHAVATAPGPWMDGVALTARQFSEAFKSAGITAIDPLGEPFDSAWHEALGH
                     APSASVPADHVVTVVSPGYRLGDRLLRPARVLISSGGATND"
     gene            complement(910..1929)
                     /gene="hrcA"
                     /locus_tag="VF720_06175"
     CDS             complement(910..1929)
                     /gene="hrcA"
                     /locus_tag="VF720_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX7878977.1"
                     /translation="MLSERDRLILSHIINQHVETAAPVGSQVLARGPAIGLSPASIRG
                     IMMDLEERGYLGQPHTSAGRVPTDKAYRAWVDGWLEPTPLDEPELERIESAFEGTPAE
                     LSSLLEQAARLLGGACRQVGVALAPEIGHAVLAGIEFTRVAEGRVLMILRLRGNAVRT
                     VLLDVSSRLESEALAATAARLNEYLAGLTLAEISATLVERSRSWPIRDEEIVTRLVAL
                     GPGLLNLGEPSALYVRGTGELAAQPEFQSPERLREILALIEERPGLSALLSERRARPG
                     IEITIGRENPSGPLTRASIVTFTWSHGALSGTVGVIGPTRMPYPRVLALLSGIAGGID
                     RRLSA"
     gene            complement(2039..3067)
                     /locus_tag="VF720_06180"
     CDS             complement(2039..3067)
                     /locus_tag="VF720_06180"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX7878978.1"
                     /translation="MTQTAHRPPDDERPIDWNPILASLAGARSIAITSHINPDGDALG
                     SELGLARWLARRGQTVRIVNSDPVPDRYDFLDRDNRIETFDPVRHRDLATECDRLVVV
                     DVSRWDRIGPVGTVMAGDALARLCIDHHPGAVDFPTVADVVAPDRAATGELIHELITA
                     AGERVDRSIAEALYVAIMTDTGSFRHASTTPHTHRIAADLLATGIDPSELYDLTWSRN
                     SETRMRVIGEAYRSFELRAGGALVVAAFDSVRLTALNATPADAEGLAEMLRSVDGCRA
                     SAVFIETAPGDVKVSLRSRGAVDVNAVARQFGGGGHVNAAGIPARGSLAEVRAAVTHA
                     LEVALAGA"
     gene            3146..3751
                     /locus_tag="VF720_06185"
     CDS             3146..3751
                     /locus_tag="VF720_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878979.1"
                     /translation="MTILAALFRPLPIVPAALLLLVPATALAGRWFDIGARQVEFVKC
                     ATDSENVESFPPDAFGIFPTGSWVYEDITEVGCDKIRWPEQVCWNQSHVRSFRVEFND
                     GGCGCGGYVAIPSTLTLRWDPEVVAARGIPETDLRLAYNDPSTRGWRPVEGALPDVEN
                     NSFTVAWGGDILGVREYAIVTPVFVPVAPSTWSRIKTLLGE"
     gene            3754..4731
                     /locus_tag="VF720_06190"
     CDS             3754..4731
                     /locus_tag="VF720_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878980.1"
                     /translation="MARGGRVVRLAGAWRLLFLATFALALVAPSRAIAQGCCTPGAGP
                     LTGLRAGGLSAFTVRGGLAWDRYDLRTTLKGSEETEPLAERKARYSRLTLQLEAGLPA
                     GMRFGVEAPWEFRNREVVLPIGGEEPARLDFDNSAFGDLTTTLVVEVAPRAATPRPWS
                     IEVGGGVKWATGSIDAEDDGFALPPELQSGTGSVDPLAVAAARWYAADWGVVGTFLYR
                     ATGTNDIGYRFGREADIGLAAWWVIVPAVSAGADIRWRSAGHDEFLDVTRPNSGGTRW
                     LAGPRLAARLPYPGPSFEVAGLFPVHQELNGTQLGVDVQWTMAISWQGR"
     gene            complement(4758..5678)
                     /locus_tag="VF720_06195"
     CDS             complement(4758..5678)
                     /locus_tag="VF720_06195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX7878981.1"
                     /translation="MNPSRAPSRIVERRRAEMILFLMVAAWGLTFPAIKVAVQDVSPG
                     LFMALRFGLVLVFLAAIGQGRYSRNLRPAWRDGLVLGLLLWGSYIFQAVGLKYTTATR
                     SGFITGLSVLIVPLIHAPLRRRLPSLRTLAAILLSLAGLWLLTRPDLGAPNLGDILTL
                     GTAVSYALYVVLMEGNAGEERRNALIGWQVLVMAAASILVMPILDGGLPGGPGTRYLT
                     MSVSHIMASPNLLIGLVVTVPVAIFSVVAITQLQRHTTAVRAGVLYSAEPLFAALFAA
                     LWLGERLDPLGLVGGGLILAGIVLAATEPD"
     gene            complement(5731..6204)
                     /locus_tag="VF720_06200"
     CDS             complement(5731..6204)
                     /locus_tag="VF720_06200"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX7878982.1"
                     /translation="MSRMAPNDNPNRAPDPGGKFVLPEILKGYSTTLREFFTKPSTMK
                     YPEEKWSMPPRFRGYPKLVPNPDGTERCVACGLCAAVCPPLAIQLQADETDRVKERFP
                     INFAIHMGRCINCGFCEEACPVDAIRMSAKYELNTPEVDALIYSKQILLKDYSTL"
     gene            complement(6201..8243)
                     /locus_tag="VF720_06205"
     CDS             complement(6201..8243)
                     /locus_tag="VF720_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX7878983.1"
                     /translation="MPKLKINDIEYEAREGATILDVARANGIDIPYMCYHPGMEVVAV
                     CRVCQVEVKGMPKLQTACSLPIRDGMEVFTESEQARKARAGTVEFWLVNHPLDCPICD
                     KGGECPLQDVTYNVRGGGSRLRDPKVNRPKRKILGEHIVFDVERCIICWRCTRFTQDI
                     SGSEQLMLSNRGVRTEIDTPPGVTLTDPFSGNLADVCPVGALTTREFRFKSRPWEMTP
                     VESTCTACSVGCSTTVWSKRGVIQRMTARENLEVNDYWLCDRGRFDTNFVAAEGRLTR
                     PTGPDSTAGDKSYSWEEASMALAGMATGRDGMAKVGSWAVVAAPHVTNEEYFALQRFA
                     RNILRTEDLILDTGTGGPELTKSRAAFLASDRRLDAVSDLDHADVIVQVGQNLEASHP
                     VYSLRLRKGVRAKKTKLYIGSTAPGGLDREASLRVGLKDGEESAFLGRIASGAGDDPI
                     RWLRDALASAKRGVIVLNAGTRNESILAAALALADLNPTGVKLLVLDDVPNTHGAWDM
                     GFAAGYGPGRRTAGNGRARTAILNAITTGSVDGLILYNAGRPAAWSADVLEAARAAKT
                     LVLFDIAGSELTARAKLVLPSPTFAETDGTMTSADGRVQLIRRAVPGPDGAWHPCQLL
                     ARAERLAGGEARSGQPVDIFREISRAAAGYQDLNFGALRPNGRPTRAAAGVTPTRT"
     gene            complement(8337..9491)
                     /gene="nuoD"
                     /locus_tag="VF720_06210"
     CDS             complement(8337..9491)
                     /gene="nuoD"
                     /locus_tag="VF720_06210"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEX7878984.1"
                     /translation="MTVNMGPQHPSTHGVLRINLELDGEIVVRAIPVAGYLHRGYEKI
                     AESKTFHQYIPYTDRLDYLAPLSNNVSYILAVEKLLGITAPTRAQWIRVMVCELARIS
                     AHLLGVGTWALDLGAQSMFFYTFREREAIYNLYEKLTGARMTTSFTRVGGIMADLPDG
                     FLDDLAGFIKQMMKKIDDYERLLTKNRIWIRRTRHIGVIGPAEAINIGLTGPALRGSG
                     VNWDLRRDRPYLVYPELEFDVVVGSAGDVYDRYLMRIGEMRQSLRILDQIVKKIPAGP
                     VNVSDPRISYPPKDRVYSSMEELIYHFKIAAEGHRAPVGEVYHAIENPKGELGFFIAG
                     DGTNHPYRLQIRSPSFLNLQSLPRLCEGHLIADVIAIIGSLDIVLGEIDR"
BASE COUNT         1459 a         3229 c         3505 g         1578 t
ORIGIN      
        1 aaggcgcgca gcgcgtcctg cagatcgaac gggtgaccgg cgccaaaccc ggcgcccttc
       61 agcccgtcgt ggccgaagcg atcgtacgcc gcccgcttct ggggatcgtg cagcacttcc
      121 caggcttcgg tggcttcctt gaagctctcc tcggccgcgg catcgcccgg gttgcgatcg
      181 gggtggtact gcatggccag tttccggtag gccttcttga tggcgtcgac gtcggcgtcc
      241 cgcgccacgc cgagcacgtc gtagtagtcg cggcgtcccg gcatcagtcg ttcgtggccc
      301 cacccgagga aatcagcacg cgggccggac gcagcagccg gtcgcccagg cgataccccg
      361 gcgagacgac ggtgacgacg tgatcggccg gcacgctggc ggacggcgcg tggccgagcg
      421 cctcgtgcca tgccgaatcg aacggttcgc cgagtggatc gatggcggtg atgccggccg
      481 acttgaacgc ctcggagaac tggcgcgcgg tgagcgccac gccgtccatc cacgggccgg
      541 gcgcggtggc cacggcgtgg gccagggcac gctcgaggtt gtccagcaca tcgaacaacg
      601 ggcttgcgag ccgctcgatg ccgagttgca ccgcctgctc gcgatcgcga tgcgagcggc
      661 ggcggaagtt gtcgaactcg gcgagcaacc tcagatagcg gtcatcagcg gcgaccccat
      721 cggcgcccgc gtgggctgca cccgggcccg cttccacgcg tgacgacgcg ccggcgcctt
      781 ccgactccgg atcgtcggtg aaaccgggat cgccgtcggg accggcctcg aactcgcgat
      841 cgtcggcatt gtcggccggc gtgcggccgt cattgaaatg agtgctcatg tcgattccat
      901 ttctcgagat caggcgctga ggcgtcgatc gattccaccg gcgatgccgg agaggagcgc
      961 caggacccgc ggataaggca tgcgggtcgg gccgatgacg ccgacggtgc ccgacagggc
     1021 gccgtgcgac caggtgaagg tgacgatcga agcgcgggtg agcggccccg atggattttc
     1081 gcggccgatc gtgatttcga tgccgggccg cgcccggcgc tcggacagga gggccgacaa
     1141 acccggccgt tcctcgatga gggccaggat ttcccgcagc cgctccggcg actggaactc
     1201 cggctgcgcc gccagctcgc cggtgccccg cacatagaga gccgagggct cgccgagatt
     1261 caggagcccg ggcccgaggg ccaccagccg tgtcacgatt tcctcgtccc ggatgggcca
     1321 gctccgggag cgctcgacca gcgtggcgct gatttcggcc agcgtgaggc cggccaggta
     1381 ttcgttgagc cgcgccgccg tggcggccag ggcctcgctc tcgagccgcg acgagacatc
     1441 cagcaggacc gtgcgcaccg cgttgccccg gagccgcagg atcatcagca cgcgcccttc
     1501 ggccacccgc gtgaactcga tgccggccaa taccgcgtgc ccgatctcgg gggccagcgc
     1561 cacgcccacc tgccggcacg cgccgccgag aaggcgggcc gcctgttcga ggaggctgga
     1621 gagctcggcc ggcgtgccct cgaaggcgct ctcgatccgt tcgagctccg gttcgtcgag
     1681 cggggtgggc tcgagccagc catccaccca ggcccggtac gccttgtcgg tcggcacccg
     1741 gccggccgag gtgtggggct gtcccaggta accccgctcc tccaggtcca tcatgatgcc
     1801 gcggatggag gccggcgaca ggccgatggc cggccctcgg gccagcacct ggctccccac
     1861 cggggcggcg gtttccacgt gctggttgat gatgtggctc aggatgagcc ggtcgcgttc
     1921 gctcagcatg atttcctctg gcactcgggt catgggagtg ccaagccgag agaggttaac
     1981 aggttcgcgg gggatcgggt caagaaggag gcgcgcagca tcggggcggc ggcgaccgtc
     2041 aggctcccgc cagggcgacc tcgagtgcgt gggtcacggc cgcccgcacc tcggccaggg
     2101 agccccgggc cgggatgccg gccgcgttca cgtgccctcc cccaccgaat tggcgggcca
     2161 ccgcgttcac gtccacggcg ccgcgcgagc ggaggctcac cttcacgtcg cccggagctg
     2221 tttcgatgaa gacggccgag gcgcggcagc catccacgct tcgcagcatt tcggcgagcc
     2281 cctcggcatc ggccggcgtg gcgttcaacg ccgtgagacg cacgctgtcg aacgcggcca
     2341 cgaccagcgc accgccggcg cgcaactcga acgatcgata ggcttcgcct atcacgcgca
     2401 tccgcgtctc gctgttgcgg ctccacgtga ggtcgtacag ctccgatgga tcgatgccgg
     2461 tggccaggag gtcggcggcg atgcggtgcg tatgcggtgt ggtggaggcg tgccggaagg
     2521 aaccggtgtc ggtcatgatg gccacgtaga gcgcctcggc gatcgatcgg tcgacgcgtt
     2581 cgccggccgc cgtgatcagc tcgtggatca attcgccggt ggcggcgcgg tcgggcgcca
     2641 cgacgtcggc gacggtcgga aagtcgacgg cgccggggtg atgatcgatg cagagtcggg
     2701 ccagggcatc cccggccatg accgtgccga ccgggccgat ccggtcccag cggctcacat
     2761 ccaccacgac cagccggtcg cactccgtgg cgagatcccg gtggcggacg ggatcgaacg
     2821 tctcgatccg gttgtcacgg tcaaggaagt cgtagcggtc gggcaccgga tcgctgttga
     2881 cgatcctcac ggtctggccc cgccgggcca gccagcgcgc caggcccagc tcggagccga
     2941 gcgcatcccc gtcgggattg atgtggctgg tgatggcgat cgagcgggcg ccagccaggg
     3001 aggcaagaat gggattccag tcgatgggtc gctcatcgtc gggcggccgg tgcgctgttt
     3061 gggtcatagg ggctccattg gaagcggcgg aggctaccac acaggacgat ggaccgcccg
     3121 cgtgccgcgg gcgcatactg tagtcatgac aatcctcgcc gcgcttttcc gaccgctccc
     3181 gatcgtgccc gccgccctgc tgctcctggt gcccgccacg gccctcgccg gacgctggtt
     3241 cgacatcggc gcgagacagg tcgagttcgt gaagtgcgcc accgactcgg agaatgtcga
     3301 gtcgttccca cccgacgcct tcggcatctt cccgaccggc agctgggtgt acgaagacat
     3361 cacggaagtg gggtgcgaca agatccgctg gccggaacag gtctgctgga accagtcgca
     3421 tgtccgatcc ttccgcgtgg agttcaacga cggcgggtgc ggctgcggcg gctacgtggc
     3481 catcccctcc acgctcacgc ttcgctggga tccggaagtc gtggcggccc gcggcatccc
     3541 cgaaaccgac ctgcgcctgg cctacaacga tccgtccaca cgcggttggc ggcccgtcga
     3601 aggcgcgctt cccgatgtcg agaacaactc gttcaccgtg gcgtggggcg gtgacatcct
     3661 cggagtgcgc gaatacgcca tcgtgacacc ggtgttcgtg ccggttgcgc cttcgacgtg
     3721 gtcgcggatc aagacgctcc tcggcgagta ggggtggcgc gcggcgggcg ggtcgtcagg
     3781 ctcgccggcg catggcggct cctgttcctc gccaccttcg cgctcgcact cgtcgcgccg
     3841 tcgcgcgcca ttgcgcaggg atgctgcacc cccggcgccg ggccgctcac cggactccgc
     3901 gccggcggcc tttcggcctt caccgtgcgc ggcggcctgg cctgggatcg ctacgatctc
     3961 cggacgacgt tgaaaggcag cgaggaaacg gagccgctag ccgaacgcaa ggcgcgctac
     4021 tcccgcctca cgctgcagct cgaagccggc ctgccagccg ggatgcgctt cggggtcgag
     4081 gctccgtggg aattccgcaa tcgcgaggtc gttctcccca tcggcggcga agagccggcc
     4141 cggctcgatt tcgacaactc ggcgttcggc gacctcacga ccacgctggt cgtcgaggtg
     4201 gcgccccgcg ccgccacacc acggccgtgg agcatcgaag tgggcggcgg cgttaagtgg
     4261 gccaccggtt cgatcgatgc ggaagacgac ggcttcgcgc tgccgccgga gctgcagtcg
     4321 ggcaccgggt cggtggatcc cctggcggta gccgccgcgc gctggtatgc ggccgactgg
     4381 ggtgtggtcg ggacgtttct ttaccgcgcc accggcacga acgacatcgg ttaccggttc
     4441 ggccgcgagg ccgacatcgg cctggcagcc tggtgggtga tcgtacccgc cgtgtcggcg
     4501 ggcgccgaca ttcgatggcg atcggcgggg cacgacgagt tcctcgacgt cacgcgcccc
     4561 aacagcggtg gcacgcgctg gctggcaggc ccgcggctgg ccgcccggct cccctacccc
     4621 ggcccgtcat tcgaagtggc gggcctgttt ccggttcacc aggagctgaa cgggactcag
     4681 cttggcgttg acgtgcagtg gacgatggcg atttcgtggc aggggcggta gggagaaggg
     4741 acgatggagc gcgggcttca gtccggttcc gtcgccgcca gcacgatccc ggccaggatc
     4801 aatcccccac ccaccagtcc cagcggatcg agccgttccc ccagccagag cgccgcgaag
     4861 agcgcggcga agaggggctc ggcgctgtag agcacgccgg cgcgcacggc cgtggtgtgg
     4921 cgctggagct gcgttatcgc cacgaccgaa aagatcgcca ccggcacggt gaccacgagg
     4981 ccgatcaaca ggttgggcga ggccatgatg tggctgacgg acatcgtgag gtacctggtg
     5041 ccgggccccc ccggaaggcc gccgtcgagg atcggcatca cgaggatcga ggcggcggcc
     5101 atcaccagca cctgccagcc gatgagcgcg ttccggcgct cctcccctgc gtttccctcc
     5161 atgaggacca cgtagagcgc gtaggaaacg gcggtaccca gggtgaggat gtcgccgagg
     5221 ttgggtgcgc ccaggtcggg ccgcgtcaag agccagagcc cggccagcga caggaggatg
     5281 gcggccagcg ttcgcaggct cggcagcctt cgtcggagcg gtgcatggat cagcggcacg
     5341 atcaggaccg acaggccggt gatgaagccg ctgcgcgtgg ccgtcgtgta cttgagaccg
     5401 acggcctgga agatgtagga gccccagaga aggagaccga ggacgaggcc atcgcgccat
     5461 gccggcctga ggttccgtga gtagcgcccc tgcccgatgg cggccaggaa gacgagcacc
     5521 aggccgaacc ggagcgccat gaagagcccg ggcgagacgt cctgcacggc caccttgatg
     5581 gccgggaacg tgagccccca ggcggccacc atgaggaaga ggatcatctc cgcgcggcgg
     5641 cgctcaacga tccgtgacgg cgcgcgggac gggttcatgc tcgtgcggtg ccgatgggcg
     5701 agccggcggt gcttcccggc gcgccgcgcg tcacagcgtg ctgtagtcct tgaggaggat
     5761 ctgcttggag tagatgaggg cgtcgacttc gggcgtgttc agctcgtact tggccgacat
     5821 gcggatggca tcgaccgggc aggcttcctc gcagaagccg cagttgatgc agcgtcccat
     5881 gtggatggcg aagttgatcg ggaagcgctc cttcacgcga tcggtctcgt cggcctggag
     5941 ctggatggcc agcggcgggc agaccgcggc gcacaggccg cacgcgacgc aacgctcggt
     6001 gccgtcggga ttggggacga gcttggggta gccgcggaag cggggcggca tcgaccactt
     6061 ctcctcggga tatttcatcg tcgagggctt ggtgaagaat tcgcgcagcg tggtcgaata
     6121 acccttcagg atctcgggaa ggacgaactt gccgccggga tcgggggcgc ggttcggatt
     6181 gtcgttgggt gccatgcggc tcatgtcctc gtcggggtca cgccggccgc ggcgcgcgtc
     6241 gggcggccgt tcggacggag cgcgccgaag ttcaggtcct ggtagccggc ggccgcccgg
     6301 ctgatttcgc ggaagatgtc caccggctgg ccgcttcgcg cctcgcctcc ggccaggcgc
     6361 tcggcacggg ccaggagctg gcacgggtgc caggccccat cgggtcccgg cacggcgcgg
     6421 cggatcaact gcacgcggcc gtcagccgag gtcatcgtgc cgtcggtttc ggcgaacgtg
     6481 ggcgatggga gcacgagctt cgcgcgggcc gtgagttccg aaccggcgat gtcgaacaga
     6541 acgagtgtct tcgcggcgcg ggcggcctcg agcacatcgg cgctccaggc ggccgggcga
     6601 ccggcgttgt agaggatcag gccatccacg ctgccggtag tgatggcgtt gaggatcgcg
     6661 gtgcgcgcgc ggccgttgcc ggcggttctg cgtccggggc cgtagccggc cgcgaagccc
     6721 atgtcccagg cgccgtgcgt gttcggcaca tcgtcgagga cgaggagctt cactcccgtc
     6781 gggttcaggt cggcgagagc cagcgccgcc gccaggatcg attcgttgcg ggtgccggcg
     6841 ttgagcacga tcacgccgcg cttcgcgctg gccagggcgt cgcgcagcca gcggatcggg
     6901 tcgtcgccgg cgccggacgc gatgcgcccg aggaacgccg actcttcccc atccttgagg
     6961 cccacgcgga gcgaggcctc acgatcgagg ccgcccggcg cggtggagcc gatgtagagc
     7021 ttcgtcttct tcgcccgtac acccttgcgc aggcggagcg agtagaccgg gtggctggct
     7081 tcgaggttct gtccgacctg cacgatgacg tcggcgtggt cgagatcact taccgcgtcg
     7141 agtcgccgat ccgaggccag gaacgcggcg cgggacttgg tgagctcggg accgccggtg
     7201 ccggtatcga ggatcaggtc ttcggtcctg agaatgttcc gagcgaagcg ctggagcgcg
     7261 aagtattcct cgttggtgac gtgcggcgcg gccacgaccg cccacgaccc caccttcgcc
     7321 atgccgtcgc ggccggtggc catgcccgcc agggccatcg aagcctcttc ccacgagtac
     7381 gacttgtcac cggcggtgga atcggggccc gtgggccgcg tgaggcgccc ttcggcggcc
     7441 acgaagttcg tgtcgaaccg gccgcggtcg cagagccagt agtcgttcac ttcgaggttc
     7501 tcgcgggccg tcatccgctg gatcacgccg cgcttcgacc agaccgtggt gctgcaaccg
     7561 acgctgcagg cggtgcaggt cgactcgacg ggggtcatct cccaggggcg gctcttgaag
     7621 cggaactcgc gcgtggtgag cgcgcccacc gggcagacat cggctaggtt gccgctgaac
     7681 ggatcggtga gcgtgacgcc ggggggggtg tcgatctcgg ttcgtacgcc gcgattgctc
     7741 agcatgagct gttcggagcc cgagatgtcc tgcgtgaagc gggtgcagcg ccagcagatg
     7801 atgcagcgct cgacgtcgaa cacgatgtgc tcgccgagga tcttccgctt gggccgattc
     7861 accttcggat cgcgcaggcg gctgccgccc ccgcggacgt tgtacgtcac gtcctgcagc
     7921 gggcactcgc ctcccttgtc gcagatcggg cagtcgagag ggtggttcac gagccagaac
     7981 tccaccgtgc cggcgcgggc cttccgggcc tgctcggact cggtgaagac ttccatcccg
     8041 tcgcgaatcg gcagcgaaca ggcggtctgg agcttgggca tccccttcac ttccacctgg
     8101 cagacgcggc agacggccac gacttccatg ccggggtggt aacacatgta cgggatgtcg
     8161 atgccgttgg cccgggccac gtcgaggatg gtggcgcctt ccctggcttc gtattcgatg
     8221 tcgttgattt ttagcttggg cattgctgtc ccctgatggg tccttcgttc agcgtctcgt
     8281 caccgaatcc gtcgcggcgg tggtcccagg tgcgaccgaa ctcccgacgc tccggtctaa
     8341 cggtcgattt cgcccagcac gatatcgagg ctcccgatga tcgcgatgac gtcggcgatc
     8401 aggtgcccct cgcagagtcg cggcagcgac tggagattca ggaacgacgg cgagcggatc
     8461 tgcaggcggt agggatggtt cgtgccgtcc cccgcgatga agaatccgag ctcacccttc
     8521 gggttctcga tggcgtggta cacctcgccc accggggccc gatggccctc ggcggcgatc
     8581 ttgaagtggt agatcagctc ctccatcgac gagtagacgc ggtccttcgg cgggtagctg
     8641 atgcgggggt cgctcacgtt gaccggtccg gccgggatct tcttcacgat ctggtcgagg
     8701 atgcgcaggc tctgccgcat ctcgccgatc cgcatgagat agcggtcgta cacgtcgccc
     8761 gcgctgccaa cgaccacgtc gaattccagt tcgggataca cgaggtacgg ccgatcgcgg
     8821 cggagatccc agttcacgcc cgaaccgcgc aatgccgggc cggtgagccc gatgttgatg
     8881 gcctcggccg gtccgatcac gccgatgtgg cgcgtccgcc ggatccagat gcggttcttg
     8941 gtgaggaggc gctcgtagtc gtcgatcttc ttcatcatct gcttgatgaa gccggccagg
     9001 tcgtcgagga aaccgtccgg caggtcggcc atgatgccgc ccacccgcgt gaacgacgtg
     9061 gtcattcgcg cgccggtcag tttctcgtac aggttgtaga tcgcctcgcg ctcgcggaac
     9121 gtgtagaaga acatcgactg cgcgccgagg tcgagcgccc acgtgcccac gccgagcagg
     9181 tgggccgaga tgcgggcgag ttcgcagacc atcaccctga tccactgcgc gcgggtcgga
     9241 gccgtgatgc cgagcagctt ctccaccgcc aggatgtacg acacgttgtt gctgagcggc
     9301 gccaggtagt cgagccggtc ggtgtacggg atgtattggt ggaacgtctt cgactcggcg
     9361 atcttctcgt agccccggtg caggtaaccg gccaccggga tggcgcgaac cacgatctcg
     9421 ccgtcgagct cgaggttgat acgaagcacg ccgtgggtgg acgggtgctg cggccccatg
     9481 ttgacggtca tggtctggtg acgggcgccc ggggccacgg ggggctcggc caggccggag
     9541 gcgctggcgc ctgtccgttc cgccggcggg ttcaccgtac cgcccggcgg cacgaatgtc
     9601 ggatgtccgc ccgggaccgc cggcgtgccg accggtactc ggccgtcgtt ctgggcgcga
     9661 ctcgggcgtt cgcccgcggg cccacccgtt gcggtgcccg gcgatccggc gggacacccc
     9721 ttctcggtcg tgctcatcgg gggtgactca tcgtgaagca ggtcgcatgt c
//
LOCUS       DASVOD010000281         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_384013, whole genome shotgun sequence.
ACCESSION   DASVOD010000281 DASVOD010000000
VERSION     DASVOD010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384013"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1979)
                     /locus_tag="VF720_06215"
     CDS             complement(<1..>1979)
                     /locus_tag="VF720_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7878985.1"
                     /translation="IHAEPRRPAWNETLAILDTGAEPETTWEVRVEALGMDGVIASRE
                     RRVRIDHSPPVITELHSTPVLSVDPVTDAVGYGLRVSAKSDEPVFGRLNVTPDGVGLP
                     PIESVPPPRFERMLSSAEPILGLDQPLAPGNWTAHASYRNDAGRSTERTTSVSIPAAS
                     VDRPVRSVAPDAATYLPRLVDIDGDGQPELVGESRLRTGPFYGNLRAWRPRGGGIPFE
                     EVWRSDERGIPRDAGDFDGDGRTDLLVLSLQKATVYSARISGGFPTFKLAEINDGWAA
                     LFIPAASGTGLDVVTSFNTSIRIFRREAGGVVLDQELMQTGNGINTISPGIVKLKDSG
                     DGLGLATVDGDGDLLIARRGGDGRFTWQQTIRLGPDYLPGIAAADLDGDGTSEIAVVE
                     AVGEVPSPTAGLRDGYYRLRILRPGEDGDYHPWQELGVTGQFPGNAVHLDGGIPRTRS
                     GEGECVWATLNGRAWRLALDEGQLAVDTLVDDVGDGPLAWGLVGEGLDTQAWTIFPTR
                     PGVSPPDDDLVSATLVGRFPSVPFHPMSPLLGLEATFVGRSLSGEQIRLAWSGGEVTS
                     VVRIPQSLDLPESPWSRTLPPGATSIVDSTAIAGHRYFYRLSAPDGSPLATLDVTASP
                     SNPVVDSSWDGRRLIVRWTHPIRPRESARLVVLPDG"
BASE COUNT          330 a          693 c          661 g          295 t
ORIGIN      
        1 ccgtccggga gcaccaccag ccgcgccgac tcgcgcggac ggatcgggtg tgtccagcgc
       61 acgatcagac ggcgaccatc ccaggaggaa tcgacgacag ggttcgaagg tgacgccgtc
      121 acgtccaggg tcgccagtgg cgacccgtcc ggggccgaaa gccggtagaa gtagcgatgt
      181 cccgcgatgg cggtgctgtc gacgattgag gtagcccccg gcggcagtgt ccgggaccag
      241 ggcgactctg gaaggtccag actctgcggt atccgaacca ccgacgtcac ctctccgccg
      301 ctccacgcca ggcggatctg ctcgccggac aggctgcggc cgacaaacgt cgcctccagg
      361 cccagcagcg gggacatggg gtgaaacggt acggatggaa accggcccac cagcgtggcg
      421 gacacgaggt cgtcatcggg aggtgacacg ccgggccgtg tcgggaagat cgtccacgcc
      481 tgcgtatcga ggccctctcc caccaacccc cacgccagcg ggccgtcgcc gacgtcgtcc
      541 accagcgtat ccacggcaag ctgcccctcg tccagggcca gtctccatgc ccgtccattg
      601 agcgtggccc acacacactc cccctcgccg gagcgggttc gcggaattcc cccgtcgagg
      661 tggacggcgt taccggggaa ctggccggtc acacccagct cctgccacgg gtgatagtcc
      721 ccgtcctcgc caggacgcag gatcctgaga cgatagtagc catctcgcag tcccgccgtg
      781 ggcgagggca cctcacccac ggcctcaacc accgcgattt ccgatgtgcc gtcgccgtcg
      841 aggtcggcgg cggcaatgcc ggggaggtag tccggtccga gccggatcgt ctgttgccag
      901 gtgaagcgcc cgtcgccgcc gcggcgggcg atcaggaggt cgccgtcgcc gtccaccgtg
      961 gccaggccga ggccgtcgcc ggagtccttc agcttcacga tgcccggcga gatggtgttg
     1021 atcccgttcc ccgtctgcat caactcctgg tcgaggacca cccccccggc ttcgcggcgg
     1081 aagatccgga tcgacgtgtt gaagctggtc acgacatcaa gcccggtgcc cgaagcggcc
     1141 gggatgaaca gggccgccca gccgtcgttg atttcggcca gcttgaacgt gggaaaacca
     1201 ccggaaatgc gcgcgctgta cacggtggcc ttctgcagcg acaggacgag aaggtcggtc
     1261 cggccgtccc catcgaagtc acccgcgtcg cgtggaatgc cgcgctcgtc actgcgccac
     1321 acttcttcga agggaatgcc accacccctc ggccgccagg cccgcaggtt tccgtagaac
     1381 ggtcccgtcc tcagcctcga ctctccgacc agttccggct ggccatcccc gtcgatatcc
     1441 acaaggcgcg ggaggtaggt ggcggcatcg ggcgcgacac tccggaccgg gcgatcgacc
     1501 gacgcggccg ggatcgaaac cgacgtggtt cgctcggtgc ttcgtccggc gtcgttgcga
     1561 taggatgcgt gcgcggtcca gtttcccggc gccaacggct ggtcaaggcc gagaatcggc
     1621 tcggcgctgg agagcatccg ctcgaagcgc ggcggtggca ccgactcgat cggcggcagg
     1681 ccgacgccat cgggtgtcac gttcaggcga ccgaacaccg gttcgtcgga cttggccgac
     1741 accctcaggc cgtacccgac ggcatcggtc acgggatcaa ccgacaggac cggggtggaa
     1801 tggagctccg tgatgacggg cggcgaatga tcgatccgga cgcgtcgctc gcgagaagcg
     1861 atcacgccgt ccatgccgag cgcctccacc ctcacctccc aggtcgtctc cggctcggcg
     1921 ccggtatcga ggatggccag cgtctcgttc caggcggggc gtcgcggctc ggcatgaat
//
LOCUS       DASVOD010000282         6297 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_540030, whole genome shotgun sequence.
ACCESSION   DASVOD010000282 DASVOD010000000
VERSION     DASVOD010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540030"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF720_06220"
     CDS             complement(<1..262)
                     /locus_tag="VF720_06220"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEX7878986.1"
                     /translation="MQEPTDSRREYQDRQKAAARSLASLECDALLSTPGTNLRYLTGL
                     TIHRMLRLAAHVLPAGGDSFMVCPAFEESNVRRVMPEGEFVGW"
     gene            360..929
                     /locus_tag="VF720_06225"
     CDS             360..929
                     /locus_tag="VF720_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7878987.1"
                     /translation="MKVAFIGTHGVGKTALAYALAAHLKRIDIAVGLVQEVARDCPMP
                     INRETTLAAQSWILHTQIAREIAAAEKNDAVICDRSVLDNYAYLVHRVGRRKSMDPLV
                     KEWLAGYTLLVKVPIWQRPRFDGVRATDVDFQREIDAEVDALVAEFGATCFRLAPREP
                     DGWIDRVIEHLQLPKEPPQQELFPEDDDA"
     gene            961..1446
                     /gene="ruvC"
                     /locus_tag="VF720_06230"
     CDS             961..1446
                     /gene="ruvC"
                     /locus_tag="VF720_06230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEX7878988.1"
                     /translation="MKVMGIDPGSQVTGWGLVHGSGNRFQSAGAGEIRAPHNEELIDR
                     LARMHREVVRLLATECPDAVALETPFFARSVHATLVLGQVRGVLLLAIRDSGIQLFEY
                     APREVKSSITGRGGATKEQVQFMVVRLLGLKADTTLDASDALAVALCHHHRARRPVLS
                     T"
     gene            1443..2048
                     /gene="ruvA"
                     /locus_tag="VF720_06235"
     CDS             1443..2048
                     /gene="ruvA"
                     /locus_tag="VF720_06235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX7878989.1"
                     /translation="MIARLAGKVIDKLADRIVLDVNGVGYEVFMPATSLARIGAAGSS
                     LVVHTVTHVREDALLLFGFLAFDEKHLFETLTGVSGVGPKLALAILSGLTPAALRGAV
                     ARRDLAALTQVSGIGRKTAERLLVELRDRVGEDVGGELDASGEATPFQDAVTALITLG
                     YARPQAVSAVRDAAGELAPDAAPEHLVRRALGRLVSSRSGL"
     gene            complement(2026..2595)
                     /locus_tag="VF720_06240"
     CDS             complement(2026..2595)
                     /locus_tag="VF720_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878990.1"
                     /translation="MTLRQALRTALVAAALLVPTRQAMAADPCFDVRVEYLGRTFYGN
                     IGGIQYWTWNYKVVGETCLNRGLSHWVLGLCANYWSLVRDISTTSTDNSDPANGTLTT
                     YTTNVGVDPPTGQAGLKWNSASGNQLDKANEYDTFSFVSPGQENLMGVAWAAKGATLV
                     EHGTTIGPSCSPVPVETSTWTKVKARFGN"
     gene            complement(2801..3526)
                     /locus_tag="VF720_06245"
     CDS             complement(2801..3526)
                     /locus_tag="VF720_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX7878991.1"
                     /translation="MLGRLLAIFAHPDDESYGPAGTLVEARARGGAVRVITATRGEAG
                     SLGISKQYDPHELARIRQGELFAAARVMDFDSVILGWPDKGVAAVDHGVAIETLVREI
                     RLFRPDSLLTFHPNGLSGHSDHIAITALALEAERFSARADWRPELGEAWRVEHHWHYA
                     IPESKAAQMADFRRVHSVPDSEVNETLDVSPHTFRKESACLAHVTQRAFLEQLRGARG
                     GLHDFWGHEHLVRAGQNGTAPLR"
     gene            3525..4532
                     /locus_tag="VF720_06250"
     CDS             3525..4532
                     /locus_tag="VF720_06250"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX7878992.1"
                     /translation="MAAKDSRLDLSDADRIWRRFEPPLMPGPGLLDRLLARGCPGARA
                     RHIDFLDRGVVNSNYRVDLEPTDSGPTTVRLRLSRSAEATAREAALADRLPDGTPRCL
                     VTEACDAPEPHVASLWTWLEGRTLEEHPNDSAGLGDDLADFLLPVHATTFPGYGRLTP
                     GLDVANAPGFHRPSARWLDDLAHRVAVRLENPAHGLAPDMTLALRTAAARVMAPLWSR
                     PPAPGSLVHGDLSPGNIIIAPDGCAVGLLDWEMARAADPAVDAASIDFELADRWPLLA
                     ARALERLAAGSPDEWERRLDLARLPLLIDARMVARVRHSAPLLERVDARLDSLVSRWS
                     G"
     gene            4556..>6297
                     /locus_tag="VF720_06255"
     CDS             4556..>6297
                     /locus_tag="VF720_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7878993.1"
                     /translation="MWKRVGIVVLVGLVARAICLWLVSQRLPFLTDPVGDAGYWLAVA
                     QGAAGGPPADPPGFITWLRLVSSGPLAAVMNLFLNSGTILAAAAIAERWFGPREALRT
                     AILLSLAAAVTFFDTQVLEAAFTAFLVTGSVLLFVLADRSTPMALTRAADRHAEQAIA
                     DGVRPTEPPRNDRVLMGLAGVTAALTAAFMPGLLLAGLLVSAWLIKTALEHRRHRSLT
                     TAWQIGLALGVLITLDWGVVRRVPVPWPAGAGVRFYLANNPEGTGLPGPIPPEFSGAR
                     ALQASESDSFEVAALGLVLEDGPALGYWTVTTFSRVFADFGGWLGLQGRKVQLLVTRI
                     DEEPGASQALEIARVPYRLAMPLPTNLLIFAGLIGLILASGGRSSRRGARAALGVSIA
                     FLLAALASALVEWPLGRLRFSVLPLLGIFAAHAMTRADDSWRSGHRRAVAAITFLLVL
                     ATALTWLSPAGPWRRPDHEASLLVTAGVSREARGDTTGAEALWSEALTLNPTHLESRM
                     RRSERALQKHDLARAITELEAARRGTTGDFSLHNNLGILYVQAQRFNDAEPELAAATR
                     LDPESPGPWYWRGQV"
BASE COUNT          904 a         2184 c         2159 g         1050 t
ORIGIN      
        1 cccagccgac gaactcccct tcgggcatca cccggcggac gttcgactcc tcgaaggcgg
       61 gacacaccat gaagctgtcg ccgccggccg gcaggacatg ggccgccagc cggagcatcc
      121 ggtggatcgt gaggccggtg aggtagcgga ggttcgtgcc gggcgtggag agcagggcgt
      181 cgcattcgag cgaggcgagc gagcgtgcgg cggccttctg gcgatcctgg tattcgcgac
      241 ggctgtcggt gggctcctgc atgctccctc cgtcctgctg tgagccacac ccgggcctgt
      301 tcccggggcg cggatgataa tccgtttcgc cgccgcgtgg cagcggctaa tatcgcgcca
      361 tgaaagtggc gttcatcggc acccacggag tcggcaagac ggccctggcc tatgccctgg
      421 cggcccacct caagcggatc gacatcgcgg tggggctggt gcaggaagtg gcccgagact
      481 gcccgatgcc gatcaacagg gaaacgacgc tggcggccca gtcgtggatc ctgcacacgc
      541 agatcgcccg ggagatcgcg gccgccgaga agaacgacgc ggtgatctgc gatcgctccg
      601 tgctcgacaa ctacgcctac ctggtgcacc gggtggggcg gcggaagtcg atggacccgc
      661 tcgtcaagga gtggctggcg ggctacacgc tgctcgtgaa ggtgccgatc tggcagcggc
      721 cgcgcttcga cggcgtgcgt gccaccgacg tggatttcca gcgcgagatc gacgccgagg
      781 tcgacgccct ggtggccgag ttcggcgcca cctgctttcg cctggcaccg cgcgaaccgg
      841 acggctggat cgaccgggtg atcgagcatc tccagctgcc gaaggagccg ccgcagcagg
      901 aactctttcc cgaggacgac gacgcatgat ccgggacggc cgcatgaccg ggaacgtcgc
      961 atgaaggtga tggggatcga ccccggcagc caggtgaccg ggtgggggct cgtgcacggt
     1021 tcgggaaacc gcttccagtc ggccggggcg ggggagatcc gcgcgccgca caacgaggaa
     1081 ctgatcgatc ggctggcccg catgcaccgc gaggtggtac ggctcctcgc caccgaatgt
     1141 cccgacgcgg tggccctcga aacgccgttc tttgcccgct ccgtgcacgc cacgctggtc
     1201 ctcggccagg tgcgcggcgt gctgctcctc gccatccgcg attccggcat ccagctcttc
     1261 gaatacgccc cgcgtgaggt aaagagctcg atcacgggac ggggcggggc caccaaggag
     1321 caggtgcagt tcatggtggt gcggttgctc ggcctcaagg ccgacaccac tctcgatgcc
     1381 tccgatgccc tggccgtggc gctctgccac caccatcggg cgcggcggcc ggtcctgtcg
     1441 acatgatcgc ccgccttgcg gggaaggtga tcgacaagct ggccgaccgg atcgtcctcg
     1501 acgtgaacgg cgtcggctac gaggtcttca tgccggccac gtcgctggcc cggatcgggg
     1561 cggccgggag ctcgctcgtg gtgcacacgg tcacgcacgt ccgcgaggat gccctgctcc
     1621 tcttcggctt cctggcgttc gacgagaagc atctcttcga gacgttgacg ggggtgagcg
     1681 gcgtggggcc gaagctggcg ctggcgatac tctcggggct cacgccggca gccctgcgcg
     1741 gcgcggtggc acggcgcgac ctggccgcgc tcacccaggt gagcggcatc ggccggaaaa
     1801 ccgccgagcg cctgctggtg gaactacgtg atcgggtggg ggaagacgtg ggcggggagc
     1861 tcgacgcgtc cggggaggcc acgcccttcc aggatgcggt gaccgccctc atcacgctgg
     1921 gctacgcgcg cccccaggcg gtttcagcgg tgcgggacgc ggccggcgaa ctcgccccgg
     1981 acgccgcgcc ggaacatctc gtccggcggg cgctcgggcg gttggtcagt tcccgaagcg
     2041 ggctttgacc ttcgtccagg tggaggtttc gaccggcacc gggctgcagc tcggcccgat
     2101 ggtggtgccg tgctcgacca gggtggcgcc cttggcggcc cacgccactc ccatcaggtt
     2161 ttcctgtccc ggcgatacga acgagaaggt gtcgtactcg ttggccttgt cgagctggtt
     2221 gccgctggcc gaattccact tgaggcccgc ctgccccgtc ggggggtcga cgccgacgtt
     2281 ggtggtgtag gtggtcaggg tcccgttcgc cgggtcgctg ttgtcggtgc tggtggtcga
     2341 gatgtcgcga acgagcgacc agtagttcgc gcagaggccg agcacccagt gcgaaagacc
     2401 ccggttgagg caggtctccc cgaccacctt gtagttccag gtccagtact ggatgccgcc
     2461 gatgttgccg tagaacgtac gtcccaggta ttcgacgcga acgtcgaaac aggggtcggc
     2521 cgccatggcc tggcgggtgg ggacgaggag ggcagccgcc acgagggcgg ttcgcagggc
     2581 ttggcgcaac gtcatgatgg gagctttcct cacagttggt ggcgtcacgg atgctgttcg
     2641 tcgtgctggc tcggcactcg aattgttcga aaaagtatag cgaggattag atgcagaggc
     2701 tcgggaaaag ttctgacttc gtgaagttaa cctctgaatt tactggggtt tccaatgatc
     2761 gatcgtcagg gaagctgaca attctaaccg cattcgaccg ctaccggagt ggtgctgtcc
     2821 cattctgccc cgctcgcacc aggtgctcgt ggccccagaa gtcgtgaagc cctccacggg
     2881 caccccgaag ctgctcgaga aaggcccgtt gggtgacgtg ggccaggcag gccgactcct
     2941 tccggaaggt gtgcggggag acatcgaggg tttcgttgac ttccgaatcg ggaacggagt
     3001 ggacgcgccg gaagtcggcc atctgggccg ccttcgactc ggggatcgca taatgccagt
     3061 gatgttcgac gcgccatgcc tcacccaact cgggccgcca gtcggcacgc gcggaaaatc
     3121 tttcggcctc cagcgccagc gcggtgatgg cgatgtgatc ggaatgtccg gaaagaccgt
     3181 tgggatgaaa cgtgaggagc gagtccggac ggaagaggcg gatttcccgg acgagggttt
     3241 cgatggccac gccgtgatcc accgcggcca ctcccttgtc gggccagccc aggatcaccg
     3301 aatcgaaatc catgacccgg gcggccgcga acagctcgcc ctgccggatg cgggccaact
     3361 catggggatc atattgcttt gagattccga gcgagccggc ctcgccgcgc gtggccgtga
     3421 tcactcgcac ggcgccccct cgggcacggg cctcgacgag cgttccggcg ggcccgtacg
     3481 attcgtcgtc cgggtgagcg aagatggcaa ggagacggcc cagcatggcg gcgaaggata
     3541 gcagactcga cctttccgac gcggaccgga tatggcgtcg cttcgagccg ccgctgatgc
     3601 ccggtcccgg actcctcgac cgcctcctcg cgaggggctg tcccggcgcc cgcgcgcgac
     3661 acatcgactt cctcgatcgc ggcgtggtca attcgaacta ccgcgtcgac ctcgaaccca
     3721 ctgactccgg cccgaccacg gtgcgcctgc gcctctcccg gtcggccgaa gccaccgctc
     3781 gtgaggccgc gctggccgat cgactgccgg acggcacgcc ccgctgcctc gtcacggagg
     3841 cctgcgacgc gccggaaccg cacgtggcgt cgctctggac ctggctcgaa gggagaacgc
     3901 tcgaggagca tccgaacgac agcgccggcc tcggtgatga tctcgccgat ttcctgttgc
     3961 cggttcacgc aaccacattc cccggttatg ggcgtctgac gccgggcctc gacgtggcga
     4021 atgcccccgg gttccaccgg ccgtccgccc gatggctgga cgacctcgcg catcgcgtgg
     4081 ccgtgcgcct cgagaacccg gcccatggcc tcgcgccgga catgacgctg gcgctccgaa
     4141 ccgcggccgc ccgggtcatg gccccgctct ggtcccggcc gccggcgccg ggatctctcg
     4201 tgcacggcga tctctcgcca ggcaacatca tcatcgctcc cgacggttgc gccgtgggcc
     4261 tgctcgactg ggagatggca cgcgcggccg atccggcggt ggacgccgcc tcgatcgact
     4321 tcgaactggc cgaccgatgg ccgctcctcg cggcccgcgc gctcgagcga ctggccgcgg
     4381 gatcgcccga cgaatgggag cggcggctcg acctggcgcg gctgccgctc ctcatcgatg
     4441 cccgcatggt ggcccgggtt cgccacagcg cgccgcttct cgaacgggtg gacgcccgcc
     4501 tcgactccct cgtttcgcgg tggtccggct gacatctcgg ctatcatccg ccgccatgtg
     4561 gaagcgggtg gggatcgtcg tgctggtggg cctcgtcgcc cgcgccatct gtctctggct
     4621 ggtgagccaa cgcctcccct tcctcaccga cccggtggga gatgccggct actggctggc
     4681 ggtggcgcaa ggcgcggccg gcggcccccc ggccgatccg ccgggattca tcacctggct
     4741 ccggctggtc tcgtcgggcc cgctggcggc ggtgatgaac ctcttcctca attcgggaac
     4801 gatcctggcc gccgccgcca tcgcggagcg atggttcggc ccgcgcgagg cgctccgcac
     4861 cgccattctc ctctctctgg ctgcggcggt gacattcttt gatacgcagg tgctcgaggc
     4921 ggccttcacg gcgttcctgg ttacgggctc cgtgctcctc ttcgtcctgg ccgaccgctc
     4981 gacgcccatg gcgctgacgc gggccgccga ccgccatgcc gaacaggcca tcgcggatgg
     5041 ggtgcgcccg actgagccgc cacgcaatga tcgtgtgctg atgggtttgg cgggggtgac
     5101 cgccgccctc accgccgctt tcatgccggg acttctcctg gccggcctcc tcgtttcggc
     5161 ctggctcatc aagacggcgc tcgagcatcg acgacaccgc tcgctgacca cggcctggca
     5221 gatcggactc gccctgggcg tcctgatcac gctcgattgg ggcgtcgtgc gtcgcgtgcc
     5281 ggtgccgtgg ccggcggggg cgggcgtccg gttctacctg gccaacaacc ccgagggaac
     5341 gggcctgccc gggccgatac cgcccgagtt ttccggcgcg cgcgccctgc aagcctcgga
     5401 atccgactcg ttcgaggtgg ccgcgctcgg gcttgtgctc gaggacgggc cggcactcgg
     5461 ctactggacc gtcacgactt tcagccgcgt gttcgccgat tttggggggt ggctcggcct
     5521 tcaggggcgc aaggtccaac tcctcgtcac gcggatcgac gaagaaccgg gggcctccca
     5581 ggcgctcgag atcgcgcgcg tcccctaccg gctcgcgatg ccgctcccca ctaatctcct
     5641 catcttcgcc ggcctcatcg gacttatcct ggcgtccggc ggtcgctctt cgcggcgcgg
     5701 cgcccgggcc gcgctcggcg tgtcgatcgc gttcctgctc gcggcgcttg cctcggccct
     5761 cgtggaatgg ccgctcggcc gacttcgctt cagcgtgctt ccgctcctcg gcatctttgc
     5821 ggcccacgcc atgacccggg ccgacgactc ctggcggagc ggccaccgcc gcgcggtggc
     5881 cgcgatcacg ttcctcctcg ttctcgcgac cgccctgacc tggctttcgc cggccggacc
     5941 gtggcgtcga cctgaccatg aggcgtcact cctggtgacg gcgggcgtgt cgcgcgaggc
     6001 ccgcggcgac accaccggcg ccgaagccct ctggagcgag gcgctgacgc tgaaccccac
     6061 tcacctcgaa tcgcgcatgc gccgcagtga gcgtgccctc cagaagcacg acctggcccg
     6121 cgccataacc gaactcgagg ccgcccgccg cggcaccacg ggcgacttct ccctccacaa
     6181 caacctcggc atcctgtacg tgcaggccca gcgcttcaac gacgccgagc ctgaactcgc
     6241 cgccgccacc cgcctcgacc ccgaatcccc cggcccctgg tactggcgcg ggcaggt
//
LOCUS       DASVOD010000283         5692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_958876, whole genome shotgun sequence.
ACCESSION   DASVOD010000283 DASVOD010000000
VERSION     DASVOD010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_958876"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VF720_06260"
     CDS             <1..352
                     /locus_tag="VF720_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7878994.1"
                     /translation="TLFALTETETRPAVFFGTSSDRIGSPEGTQSYYVTGSKYLPILH
                     LAPYATINYSEWDEGVNFPFGATLELGQGFSVRPMYDGQRTHLLGSWFSGQWGFTLIW
                     AWLEEPGAAVSFGF"
     gene            363..1157
                     /locus_tag="VF720_06265"
     CDS             363..1157
                     /locus_tag="VF720_06265"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEX7878995.1"
                     /translation="MNKTTWLVLTAFLWLFGNDSGSLAQAAVEEMDDVDVIETVQVDS
                     ADDDAAAGSGGGKTAASDVTHPGKAECAVCTAKGGAHGVEKVAGMSQYQGAWYYFCSA
                     DCKKEFDADPASFVVLPLPRPAPEINVHINGEDLPLSEFKGSVVLIDFWATWCKPCVK
                     MMPNLQKLYDKKKLEGLTVLGISIDEDAGKAVTMVADKDLTYPIVIDTGERPSWKAFG
                     VKAIPATFLVDADGKIVAQWTGNEIKWDKVEKEVERVVAESKARSN"
     gene            1176..2840
                     /locus_tag="VF720_06270"
     CDS             1176..2840
                     /locus_tag="VF720_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7878996.1"
                     /translation="MTPEQELEHLRFLDELEAHLRQVSDARKALSAALRVTREHFGAA
                     EGIIAIKRPGSHSVAPTIVWPRASRWDLGWLDGFIDTRKARIPTDTILAPVDRYGRAW
                     GAIGLRRNEAPFPKGTWRWLHRASFLVSEALAHIDRDRMIDVRDRLDRKIMEQLRPKD
                     LFYQILDGLRSLTGYDHSSTLLFREDDDAAFTIVAEQIAWTKGKSRRIGQRLTPDEGV
                     EKILQRREVLGFDREGDTWRERRWQIVDGLAGMLDYNIAPGEEREAAMLVAPIVSKTG
                     VFGLLKIASRHAGSFGRYEAELVERFRSQATIAILNSQRTEFLHARLVEAEKKHAMAD
                     LARGVAHDVNNALGSVVPIVQAMREEVTGGQIEPQVFAEDLKQIETSLLTCRRIFGGM
                     LSFARSGHRTTGVADLGAAVNATLAIMRDGLERRGITLDVDMPKEVPLLAGGQSDVEQ
                     VLLNLVTNARDAMPQGGTLGIRAARQDGLVEVVVSDTGEGIPKELLARVQEPFFTTRS
                     QGSGLGLSIVRSILWEMNGRMVLESEPGHGTSVRFYVPAAPTEVAR"
     gene            2837..3952
                     /locus_tag="VF720_06275"
     CDS             2837..3952
                     /locus_tag="VF720_06275"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7878997.1"
                     /translation="MKPARLLVVDDEPGMLRAVERVLGRRHQVASYESPLDALAAARD
                     YDPDLAILDVRMPNMDGFELMGRLQELVPGIDTILMTGSASEPDQKLIRAVRENAFYF
                     IQKPFDSEVLRTLVERCLELRQLADENKRHVRRLEGELSQAQAFQRSLLPRPEALING
                     IALAGRYQPTSELGGDFFDFARVGTTGAALVIADVSGHGVSAAMLTGVVKAGFRAAQV
                     DDFDPVAVVEQVRASLRGFGYDQFITMVAARITGDTLEYVSAGHPSTLVRTPAGLTVL
                     DSTGPIVSPVFPSGSWATTRHAFGPGASLVLYTDGISEARSPADELFGDERVAATVAA
                     FPGNGAPLLDALLNVVRAWSAGRPMADDLTLVTASRG"
     gene            complement(3962..>5692)
                     /locus_tag="VF720_06280"
     CDS             complement(3962..>5692)
                     /locus_tag="VF720_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7878998.1"
                     /translation="DIKPALMVPDLEALLLQVIESVQPVEREVRDRDDRWHSLRLYPY
                     RTLDHKIEGAVLVLADIDQIRRDGELLRHQAALIELSQDAVIVRDAENRVTIWNRGAQ
                     DMYGWTSDEARGKLLNELLGIGGPVWTEINDALHSLGKWEGEIRVQRRDNGSVTIHNR
                     EVLVRGEDGSRAEVLAIQRDVTERKRTLDVLKQADRRKDEFLATLAHELRNPLSPIRN
                     AVEVMRLSSDDPLIQAEARDVLYRQVRQMSRIVEDLIDVSRIVEKKVELRVEAVPMGA
                     VMETAVESCRSAIEAYRHHLHVSLPPRPVTLMGDSVRLSQVVINLLTNAAKYTPPGGD
                     IWLSAEVAKEGELPLPEGIPAGRSTLIVRVRDNGIGIAPEFLPRIFDLFSQAETTSEA
                     TRSGLGVGLSLVRSLVEMHGGYVKVKSVGRGTEFTLRLPIVAAPSEVGRRPRTNTPVS
                     DQEIAPRCILLVDDNKDQADSLAMLLRLQGHQVEVAYDGRSGYQKALTMRPDVALLDI
                     GMPELNGYEVARHIREHPELAEMILIAQTGWGQEEDRERSAEAGFDHHLVKPIDMNVL
                     RGLMSKTTAG"
BASE COUNT          923 a         1923 c         1904 g          942 t
ORIGIN      
        1 cacccttttt gcgctcaccg aaaccgaaac gcggccggcg gtgttcttcg gcaccagttc
       61 ggaccggatc ggctcgccgg aaggaaccca gtcgtattat gtaacgggtt ccaagtacct
      121 gccgatactt cacttagcgc cctacgccac gatcaattac tcggagtggg acgagggggt
      181 caattttccc ttcggcgcca cgctcgagct gggacagggt ttctcggtcc gaccgatgta
      241 cgatgggcag cgcacgcacc tgctgggcag ctggttcagc gggcagtggg ggttcaccct
      301 catctgggcc tggctggaag agcccggggc ggcggtttcg ttcggcttct gaaggagtgt
      361 cgatgaacaa gacgacctgg ctggtcctga ccgcgttcct ctggctgttc ggcaacgact
      421 cgggatcgct ggcgcaggcc gccgtggaag agatggatga cgtcgacgtg atcgagaccg
      481 tgcaggtcga ttcggccgac gacgacgcag ccgccggatc cggcggcggg aagacggctg
      541 cctccgatgt gacgcatccc gggaaggccg agtgcgcggt gtgcaccgcc aaggggggcg
      601 cgcatggcgt cgagaaagtg gcggggatga gccagtacca gggcgcctgg tactacttct
      661 gttccgccga ctgcaagaag gagttcgacg ccgaccctgc ctcgttcgtc gtgctcccgc
      721 tgccccgtcc cgcgccggag atcaacgtac acatcaacgg cgaggatctc ccgctctcgg
      781 agttcaaggg ctcggtcgtg ctcatcgatt tctgggccac ctggtgcaag ccgtgcgtga
      841 agatgatgcc caacctccag aagctgtacg acaagaagaa gctcgagggg ctgaccgtcc
      901 tgggcatctc gatcgacgaa gatgcgggga aggcggttac gatggtggcc gacaaggacc
      961 tgacctaccc gatcgtgatc gacacgggcg agaggccctc ctggaaggcg tttggtgtga
     1021 aggccattcc cgccaccttc ctggtggatg cggatgggaa gatcgtggcc cagtggaccg
     1081 gcaacgagat caagtgggac aaggtcgaga aggaagtgga gcgcgtggtg gccgaatcga
     1141 aggcccgctc gaactagcgt atccttgccg gcgccatgac gcccgagcag gaacttgaac
     1201 acctccgctt cctcgacgaa ctcgaggccc acctccgcca ggtcagcgat gcgcgcaagg
     1261 cgctctccgc cgcgctccgc gtaacccgcg agcacttcgg ggccgccgaa gggatcatcg
     1321 ccatcaagcg gccggggagc cacagcgtcg cgccgacgat cgtgtggcca cgtgcgtctc
     1381 gctgggacct gggttggctc gacggattca tcgacacccg caaggcgcgc atcccgaccg
     1441 acacgatact ggctcccgtc gatcgctacg ggcgggcctg gggcgccatc ggcctccgcc
     1501 ggaacgaggc tcccttcccg aagggcacct ggcgctggct gcatcgcgcg tcgtttctcg
     1561 tgtcggaagc gctcgcgcac atcgatcgtg accggatgat cgacgtgcgc gaccgcctcg
     1621 accggaagat catggagcag ctgcggccga aggacctctt ctaccagatc ctggacggcc
     1681 tgcgttcgct gaccggctac gaccattcgt ccacgctcct cttccgcgag gacgatgacg
     1741 cggccttcac catcgtggcc gagcagatcg cctggacgaa gggaaagagc cggcgcatcg
     1801 gccagcgcct cacgcccgac gagggcgtgg agaagatctt gcagaggcgc gaggtgctgg
     1861 gcttcgaccg cgagggtgac acctggcgcg aacggcgctg gcagatcgtg gatgggttgg
     1921 ccggcatgct cgactacaac attgcgccag gcgaagagcg ggaggcggcc atgctggtgg
     1981 cgccgatcgt ctccaagacg ggcgtgttcg gcctcctcaa gatcgccagc cgccacgccg
     2041 gcagcttcgg acgctacgag gccgagctgg tggaacggtt ccgctcgcag gccaccatcg
     2101 ccatcctcaa ttcgcagcgc accgagttcc tgcacgcgcg gcttgtcgaa gccgaaaaga
     2161 agcacgcgat ggccgacctg gcgcgcggcg tggcgcacga cgtgaacaac gcactcggct
     2221 cggtggtgcc gatcgtgcag gccatgcgcg aagaggtgac cggggggcag atcgagccgc
     2281 aggtgttcgc cgaggacctg aagcagatcg aaacctcgct cctcacctgc cggcgcatct
     2341 tcggcggaat gctgtcgttt gcccggagcg gccatcgcac gacgggtgtg gcggacctcg
     2401 gcgccgcggt caacgccacg ctggccatca tgcgcgacgg cctcgagcgg cgcggcatca
     2461 cgctggacgt cgacatgccg aaggaggtgc cactgctggc gggcgggcag agcgatgtcg
     2521 agcaggtgct gctgaacctc gtgaccaatg cgcgcgatgc catgccgcag ggagggacgc
     2581 tcggcatccg cgcggcgcga caggacgggc tcgtggaggt ggtggtgagc gacaccggcg
     2641 aggggattcc gaaagagttg ctggcgcgcg tccaggaacc cttcttcacc acccgaagcc
     2701 aggggagcgg cctcggtctg tcgatcgtcc gctccatcct gtgggagatg aacgggcgca
     2761 tggtcctcga atccgaaccc gggcacggca ccagcgtccg gttctacgtt cccgccgccc
     2821 cgacggaggt tgcccgatga aaccggcccg cctgctcgtc gtggatgacg aacccggcat
     2881 gctccgcgcc gtggagcgcg tcctcggccg ccgccaccag gtggccagct acgaatcgcc
     2941 gctcgatgcc ctggccgccg cccgcgacta cgaccccgat ctcgccatcc tggacgtgcg
     3001 catgcccaac atggacggct tcgagctgat gggcaggctc caggagctgg tgccgggcat
     3061 cgacaccatc ctcatgaccg gcagcgccag cgagcccgac cagaagctga tccgcgccgt
     3121 gcgcgaaaac gccttctact tcatccagaa accgttcgac agcgaagtgc tccgcacgct
     3181 ggtggagcgc tgcctcgaac tccgccagct cgcggacgaa aacaagcggc acgtgcgccg
     3241 cctggaaggg gaactttcgc aggcacaggc cttccagcgc agcctgttgc cgcggcccga
     3301 ggcgctcatc aacggcattg cgctggccgg gcgctaccag cccaccagcg aactgggcgg
     3361 cgacttcttc gatttcgcac gggtcggcac gaccggcgcc gcgctcgtca tcgccgacgt
     3421 ctcgggccat ggcgtgtcgg ccgccatgct caccggcgtg gtgaaggccg gtttccgggc
     3481 ggcgcaggtc gatgacttcg atccggtggc ggtggtcgag caggtgcgcg cctcgctgcg
     3541 cggtttcggc tacgaccagt tcatcacgat ggtggccgcc cggatcacgg gggatacgct
     3601 ggagtacgtg agcgccgggc atccgtccac actcgtccgg acgccggccg gcctcaccgt
     3661 cctcgactcg accgggccga tcgtctcgcc cgtgtttcca tccgggtcat gggcaaccac
     3721 gcgccacgcg ttcgggcccg gcgcttcgct ggtgctctac accgacggca tttccgaggc
     3781 gcgctcaccc gccgacgagc ttttcggcga cgagcgggtc gccgccacgg tggcggcctt
     3841 tccgggaaac ggcgccccgc tcctggatgc cctgctcaac gtggtgcgtg cctggtcggc
     3901 cggccggccg atggcggatg acctgacgct ggtgacggcg agccggggct gacccgctcc
     3961 ttcagcccgc cgtcgtcttc gacatcaacc cgcgcagcac attcatgtcg atcggcttca
     4021 ccagatggtg gtcgaagccg gcctccgcgg aacgttcgcg gtcttcctcc tgtccccagc
     4081 cggtctgggc gatcaggatc atctcggcca gctcggggtg ctcgcggatg tggcgggcca
     4141 cctcgtaccc gttgagctcg ggcatgccga tgtcgagaag cgccacgtcg ggacgcatcg
     4201 tcaacgcctt ttggtacccc gacctgccgt cgtaggccac ctccacctgg tgcccctgga
     4261 ggcgcagcag catggccagg ctgtcggcct ggtccttgtt gtcgtcgacc agcaggatgc
     4321 atctcggcgc gatctcctgg tccgacacag gagtgttcgt ccgcggccgg cgcccgacct
     4381 ccgacggcgc cgccacgatg ggcaggcgga gcgtgaattc ggtgccgcgg cccacacttt
     4441 tcaccttcac gtagccgccg tgcatctcga cgaggcttcg gaccaggctc agccccacgc
     4501 cgaggccgct gcgagtggcc tccgaggtcg tctccgcctg gctgaagagg tcgaagatcc
     4561 gcggcaggaa ctcgggggcg atgccgatcc cgttgtcgcg gacgcgcacg atcaacgtgc
     4621 tccgcccggc cggaatgcct tcggggagcg gcagctctcc ctccttcgcc acctcggccg
     4681 aaagccagat gtccccgccg ggcggcgtgt acttggccgc attcgtcagg aggttgatca
     4741 ccacctggct cagccggacc gaatcgccca ttagcgtgac cgggcgcggc ggcagcgaca
     4801 cgtggaggtg gtggcgatac gcttcgatcg ccgaccggca cgactcgacg gccgtttcca
     4861 tcaccgcacc cataggcacc gcttccacgc ggagctcgac cttcttctcg acgatccggc
     4921 tgacgtcgat caggtcttcc acgatgcgcg acatctggcg cacctggcga tagaggacat
     4981 cgcgcgcctc ggcctggatc agcgggtcgt cgctgctgag ccgcatgact tccaccgcgt
     5041 tccggatcgg tgacaagggg ttccgcagtt cgtgggccag cgtggccagg aattcatcct
     5101 tccggcgatc ggcctgcttc aggacgtcga gcgtccgctt ccgctcggtc acgtcgcgct
     5161 ggatggccag cacctcggcg cgcgagccgt cctccccgcg caccagaact tcgcggttgt
     5221 ggatggtcac cgagccgttg tcccggcgct ggacccggat ttcgccttcc cacttgccca
     5281 gcgagtggag ggcatcgttg atctccgtcc agacgggacc gccgatcccg agcagctcgt
     5341 tcaggagctt gccccgcgcc tcgtccgagg tccagccgta catatcctgg gcgccgcgat
     5401 tccagatcgt gacgcgattc tcggcgtcac gcacgatgac ggcgtcctgg gagagctcga
     5461 tcaacgcggc ctggtggcgc agcagctccc cgtcccgacg gatctggtcg atatcggcca
     5521 gcacgaggac cgccccctcg atcttgtggt cgagcgtgcg gtacgggtaa aggcgcaacg
     5581 aatgccagcg atcgtcccga tcgcgcacct cgcgttccac gggctggacc gattcgatga
     5641 cctggagcag cagcgcctcg aggtccggca ccatcagcgc gggcttgatg tc
//
LOCUS       DASVOD010000284         8368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_280801, whole genome shotgun sequence.
ACCESSION   DASVOD010000284 DASVOD010000000
VERSION     DASVOD010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8368
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280801"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VF720_06285"
     CDS             complement(<1..773)
                     /locus_tag="VF720_06285"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005601458.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX7878999.1"
                     /translation="MSTSGKNVGKILQVIGPTVDIEFESDALPNILNAITIQDGDRLL
                     TVEAATHVGDNVVRCVAMASTDGLKRGMEATNTGRPIAVPVGEQSLGRLFNLLGQTID
                     GKGPVTNPNQTLPIHRKAPTFDEQSTEKAILETGIKVVDLLAPYAKGGKIGLFGGAGV
                     GKTVLIQELIRNIATEHGGYSVFAGVGERTREGNDLYLEMSESGVIAKTVMVFGQMNE
                     PPGARLRVALSGLTQAEYFRDEQGKDVLLFVDNIFRFTQA"
     gene            complement(788..1639)
                     /gene="atpG"
                     /locus_tag="VF720_06290"
     CDS             complement(788..1639)
                     /gene="atpG"
                     /locus_tag="VF720_06290"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEX7879000.1"
                     /translation="MATLQDIKRRLRSVENTKQITAAMEMVSAAKLRRAQTRVTAARP
                     YAAKLSEIMENLAGVASGLAHPLFESRPEKTIGLVIVTASKGLCGSYNSQLLRRAEAW
                     LKDKPKESVRLFCVGRRGHDYFLKRGWTISGYWPDVNDLSGADSAGRVASAVIRAYRD
                     GSVDAVYLLYTKFINAMSRTITLDKLLNIEPPAERPVSGNYIFEPDPAAILGVLPERY
                     VATRLLAAFLESAASEHGARMVAMGSASRNATELISALILQRNRARQAAITKELAEIV
                     GGAEALK"
     gene            complement(1644..3158)
                     /gene="atpA"
                     /locus_tag="VF720_06295"
     CDS             complement(1644..3158)
                     /gene="atpA"
                     /locus_tag="VF720_06295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX7879001.1"
                     /translation="MAFRPEEVSAILQKEIEGHQSSLQLESRGYVLQVGDGIARVWGL
                     KDAQAGELLKFPGDVMGLVLNLEEDNIGCALFGSDVGIKEGDTVVRTGRIAEVPVGDA
                     LAGRVVNALGQPIDGKGPIATERTRLIESNAPSVVDRQPVKEPLQTGLKAIDSMIPIG
                     RGQRELIIGDRQTGKTAIAIDTIINQKTTGVRCVYVAVGQKDSTVANVVATLEKNGAM
                     AYTTVVVASASEPAPLQYIAPYTGCAIGEDVMERGGHALCIYDDLSKHAVAYRQLSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDEKGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLESDLFYSGVRPAINVGISVSRVGGNAQTKAMKAVAKSLRLDLAQVREL
                     AAFAQFGSDLDATTKKQLIRGERMVELLKQGQFSPFLLERQVMIIYAGGKGHLDDLPT
                     ADVGRFEKEFFAFTDKEYPEIAMDIAKTRDLTKETGERLEKALVTFKERFKKSA"
     gene            complement(3218..3769)
                     /gene="atpH"
                     /locus_tag="VF720_06300"
     CDS             complement(3218..3769)
                     /gene="atpH"
                     /locus_tag="VF720_06300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX7879002.1"
                     /translation="MQDAIIARKYATALFDAAVKANALEQVASDLAGVRLLNRAGSTG
                     LGAFLEAPHITTDTKRTVLDKSLKGKLSDLTLRFLHLLIDKKRSAQLDGIAAHFEELV
                     RDHKGIVAARVRTAEPLDAAQLEKMASGLGKMIGKTVEIENKVEPEVLGGVVLEFGTW
                     IVDRSVRRGLDDIRDRLMKAHVL"
     gene            complement(3769..4269)
                     /gene="atpF"
                     /locus_tag="VF720_06305"
     CDS             complement(3769..4269)
                     /gene="atpF"
                     /locus_tag="VF720_06305"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX7879003.1"
                     /translation="MDVQWKVILTQILGFLIVFLVLRKAAWGPVLKLLEDRREKIRAS
                     FADIDAQKAEVAALRVQYEAELKTIDAQARQRMNEALAEAQKLAGEIEASARERSRQE
                     MEKLKGDVDREYQAARVKFKEEMVTIALGAAEKMVRESLDRNKQSKLVDQFLAELDQA
                     GKNRKV"
     gene            complement(4346..4567)
                     /gene="atpE"
                     /locus_tag="VF720_06310"
     CDS             complement(4346..4567)
                     /gene="atpE"
                     /locus_tag="VF720_06310"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015024380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEX7879004.1"
                     /translation="MNLLGLGLPLGVGLAALGSGIGLGKAVGGAMEAMGRQPEAIPRI
                     QTAMVIGAAFIEALTIYALLTVLLLQGKL"
     gene            complement(4583..5713)
                     /gene="atpB"
                     /locus_tag="VF720_06315"
     CDS             complement(4583..5713)
                     /gene="atpB"
                     /locus_tag="VF720_06315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX7879005.1"
                     /translation="MKRLSSPVGYALAVLAMLALPAGIALTQEHGHGAAATTETHATE
                     AHSADEHATPADAHGTEAHATEAHGADAPATDAHATDAHAAGGHTVEHDPVQEAFHTA
                     ENSSFVDLILKQMGYVKPHEGEEDHNSPALKTLAMFQMPIISAVVIFLIMGVLVAGAS
                     KREMIPGKHQNLVELIIGGFDEFIQGILGLEGRHFLPFLGTLFLYIWVSNLIGLVPLF
                     KSPTSSFNTTVALAICVFLYVHWTGLRRNGIVGYLYHLAGEPKDPIGWGMVPLMFPLH
                     VIGELAKPLSLSLRLFGNIFGEETLIAVFIGLGIGALSFLPMAPVGIPFQVPFMFLGL
                     LTCTIQALVFTLLSTIYFALMLPHHDHDHDHGHGEGHAAAHH"
     gene            complement(5710..6111)
                     /locus_tag="VF720_06320"
     CDS             complement(5710..6111)
                     /locus_tag="VF720_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879006.1"
                     /translation="MGPEFLGRIVKTSLMLSVGLALVAGWYFGVDRGVDFGFAALWGT
                     LNLKMLQKVIVEATRPGGLRMEALVSAFLVKFPLLYGTGAVWMLKRHPDPAATFAGLS
                     LVLAVILLKSLGRLLMDSAWFSRPMAGGEDR"
     gene            complement(6162..6419)
                     /locus_tag="VF720_06325"
     CDS             complement(6162..6419)
                     /locus_tag="VF720_06325"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX7879007.1"
                     /translation="MSNGNRQRYSSLRQVGLLTTIPLLLLAAPVVGYLIGLKLDHWFK
                     TSFWVWIFCAIGMVAGIREVIAILKKATAESEAETRRDDAD"
     gene            complement(6576..8162)
                     /locus_tag="VF720_06330"
     CDS             complement(6576..8162)
                     /locus_tag="VF720_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7879008.1"
                     /translation="MSGTNGTNGNSKPRSPETILVAHGDPRLCELLQLTLASAGYEVR
                     VAVNAEEALSHHRRTSPELSLIDLGMVAREGVNFVEMLQGKRSGRRVPMIVISDLSPP
                     ETARQGITIGAEDFLVRPFSPPELLLRVRQSLNRLADSRALSRAHDDLDGILRRTQQE
                     LETLRQRVRVQKGGLQTLLDFNRRLDPADDEADLERTCLTQAALLAHSAGVCLFRPED
                     GQLSCLSPARWQGIAGDQVSGLRLPTDGEFLGILASTGGPVRLTEFDRLPGTSFEAGT
                     LSAAGFSIAAPLVLRGELVGLLAMGEDARGESGADLEAIGLLAGSVAHLLEAARLRRH
                     ERDVAVDTMGALVDRLEGRYPDLAGHSRRVTQMALAAGRRLQLSNDELEVLRAAGQLH
                     DLGRLLNDTDILVRPGPLSEEDWERVRRHPGDAARIAAEARWPMSVQSAILHHRERWS
                     GDGYPHGLKKDAIPLPARILGAADSLDAMTSPRPYRLPMAENDARRELMAESGRRYDP
                     VVIEAILSLDEDVDGLDQAV"
     gene            complement(8164..>8368)
                     /locus_tag="VF720_06335"
     CDS             complement(8164..>8368)
                     /locus_tag="VF720_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879009.1"
                     /translation="EAIVTADGAPAAVFLERRRLVSRARKALGQVERELSAVRLRMGG
                     APVERLTEADFVEPGHGENGQEI"
BASE COUNT         1368 a         2714 c         2830 g         1456 t
ORIGIN      
        1 gcctgcgtga agcggaagat gttgtccacg aagaggagga cgtccttccc ctgctcgtcg
       61 cggaagtact cagcctgggt gaggcccgag agagccacgc gcagacgggc tcccggaggc
      121 tcgttcatct ggccgaagac catgacggtc ttggcgatga cgcccgactc ggacatttcg
      181 aggtaaaggt cgtttccttc gcgcgtacgc tcgccgacac cggcgaacac cgagtaaccg
      241 ccgtgctcgg tggcgatgtt gcggatgagc tcctggatga gcaccgtctt gccgacgccg
      301 gcgccgccga agaggccgat cttgccgccc ttggcgtacg gcgcgaggag gtccaccacc
      361 ttgatgccgg tctcgaggat ggccttctcg gtcgactgct cgtcgaacgt gggagccttg
      421 cggtggatcg gcagcgtctg gttcgggttc gtcaccgggc ccttgccgtc gatcgtctgg
      481 ccaaggaggt tgaacagccg gccgagcgac tgctcgccga cgggaacggc gatcggccgg
      541 cccgtgttgg tggcttccat cccgcgcttc aggccgtcgg tggaggccat ggccacgcag
      601 cggacgacgt tgtcgccgac gtgggtggcc gcctcgaccg tcaggaggcg atcgccgtcc
      661 tggatggtga tggcgttcag gatgttcgga agcgcatccg actcgaactc gatgtccacc
      721 gtcggaccga tgacctggag gatcttgccg acgttcttgc cgctcgtgct catgaggtct
      781 cccattgcta tttcagcgct tccgcgccgc cgacgatttc cgcgagttcc ttggtgatgg
      841 ccgcctggcg cgcccggttg cgctggagaa tcagcgccga aatgagctcc gtggcgttgc
      901 gcgaggccga gcccatggcc accatgcgcg cgccgtgctc ggaggccgcc gattcgagaa
      961 aggcggccag gaggcgcgtg gcgacatagc gttccggcag gacgccgagg atggcggccg
     1021 gatcgggctc gaagatgtaa ttgccggaga cgggccgttc cgccgggggc tcgatgttca
     1081 ggagcttgtc cagcgtgatc gtgcgcgaca tggcattgat gaacttcgtg tagaggaggt
     1141 acaccgcgtc gacactgccg tcgcggtagg cccggatgac ggccgacgcc acccggccgg
     1201 ccgagtcggc ccccgagagg tcgttcacgt cgggccagta gccggaaatc gtccagccac
     1261 gcttcaggaa atagtcgtgg ccgcgccgcc ccacgcagaa gagccgaacg ctttccttcg
     1321 gcttgtcctt gagccacgcc tcggcccggc ggaggagctg cgagttgtac gaaccgcaca
     1381 gccccttgct ggccgtcacg atcacgaggc cgatcgtctt ctcggggcgc gactcgaaca
     1441 gcggatgcgc gagtcccgac gccacgccgg ccaggttctc catgatctcg gagagcttgg
     1501 cggcgtacgg acgggccgcg gtcacgcgcg tctgggcacg ccgcagcttg gcggccgaca
     1561 ccatttccat ggcggccgtg atctgcttcg tgttttcgac ggagcggagc cgccgcttga
     1621 tgtcctgaag tgtggccatc tcgttatgcg ctcttcttga accgttcctt gaacgtcacg
     1681 agcgccttct cgaggcgttc gccggtttcc ttcgtcaggt cgcgcgtctt ggcgatgtcc
     1741 atcgcgatct cgggatattc cttgtccgtg aaggcgaaga actccttctc gaagcggccg
     1801 acgtccgccg tcggcagatc atccaggtgc cccttgccgc cggcgtagat gatcatgacc
     1861 tggcgttcga ggaggaacgg cgagaactgt ccctgcttca ggagttcgac catgcgctcg
     1921 ccgcggatga gctgcttctt ggtggtggcg tcgaggtcgg agccgaactg cgcgaaggcc
     1981 gccagctcgc ggacctgcgc gaggtcgagg cgaaggctct tggccaccgc cttcatggcc
     2041 ttggtctgcg cgttgccgcc gactcggctg accgagatgc cgacgttgat ggccggccgc
     2101 acgcccgagt agaagaggtc cgactccagg aagatctggc cgtccgtgat cgagatcacg
     2161 ttggtcggga tgtaggccga cacgtcgccg gcctgcgtct cgatgatcgg aagcgccgtg
     2221 agcgatccgc cacccttctc gtcgttcagc ttggcggcgc gctcgaggag acgcgagtgc
     2281 aggtagaaga cgtcgcccgg ataggcttcg cggcccggcg ggcggcggag cagcagcgac
     2341 agctggcggt aggccacggc gtgcttcgac aggtcgtcgt agatgcacag ggcgtggccg
     2401 ccgcgctcca tgacgtcttc gccgatggcg cagccggtgt agggcgcgat gtactgcagc
     2461 ggggccggct cggaggccga ggccaccacc acggtcgtgt aggccatggc gccgttcttc
     2521 tcgagcgtcg ccaccacgtt ggccaccgtg gagtccttct ggccgacggc cacgtatacg
     2581 cagcggacac cggtggtctt ctggttgatg atcgtgtcga tggcgatggc cgtcttgccg
     2641 gtctggcggt cgccgatgat cagctcgcgc tggccgcggc cgatcgggat catcgagtcg
     2701 atggccttga ggcccgtctg gagcggctcc ttcaccggct ggcgatcgac cacgctcggc
     2761 gcgttcgatt cgatgagccg ggtgcgctcg gtggcgatcg gtcccttgcc gtcgatgggc
     2821 tggccgagcg cattgacgac gcggcccgcg agggcatcgc cgacgggtac ctcggcgata
     2881 cgtccggtgc ggacgacggt gtcaccttcc ttgatgccga catcggagcc gaagagggcg
     2941 cagccgatgt tgtcttcctc gaggttcagg acgaggccca tgacgtcgcc ggggaacttg
     3001 agcagctcgc cggcctgggc atccttgagc ccccagacgc gggcgatgcc gtcgcccacc
     3061 tggagcacgt atccgcggct ttccagctgg agcgaggact ggtgtccctc gatctccttc
     3121 tggaggatcg cgctgacttc ttcgggtcga aacgccatgg gtacaacctc tccgcgggcc
     3181 cgtccgtcgg gacgcgccgt aagggaccag cgcctgccta gaggacgtgc gccttcatca
     3241 accgatcacg gatatcgtcc agcccgcgcc ggacgctccg atccacgatc catgttccga
     3301 attcgagcac gacgccacct aagacctcgg gctcgacctt gttctcgatt tccaccgtct
     3361 tgccgatcat cttccccagc ccggacgcca tcttttccag ctgggccgcg tcgagcggct
     3421 cggccgtgcg cacccgcgcc gccacgatcc ccttgtggtc acgcaccagc tcctcgaagt
     3481 gcgcggcgat tccgtcgagc tgggccgagc gcttcttgtc gatgagcagg tgcaggaacc
     3541 gaagggtgag atcggacaac tttcccttga gcgacttgtc cagcaccgtc cgcttggtgt
     3601 cggtggtgat gtgcggcgcc tcgaggaacg cacccagccc ggtcgagccc gccctgttga
     3661 gcagccggac accggccagg tcggacgcca cctgctcgag cgcgttggcc ttgacggccg
     3721 cgtcgaacag ggcggtggcg tacttgcggg cgatgatcgc gtcctgcatc agaccttccg
     3781 gttctttccg gcctgatcga gctcggccag gaactggtca accagcttcg actgcttgtt
     3841 gcgatcgagc gattcccgga ccatcttctc ggctgcgccg agggcgatgg tcaccatctc
     3901 ttccttgaac ttcacgcggg ccgcctggta ctcgcggtcc acgtcgccct tcagcttttc
     3961 catttcctgc cggctccgct cgcgggcgga tgcctcgatt tcaccggcga gcttctgggc
     4021 ttcggccagc gcctcgttca tgcgctggcg cgcctgggca tcgatggtct tgagctcggc
     4081 ctcgtactga acccggagcg ccgccacttc cgccttctgg gcgtcaatgt cggcgaacga
     4141 cgcgcggatc ttctcgcggc gatcttcgag cagcttgagg accggccccc acgccgcctt
     4201 gcgcaagaca aggaagacga tgaggaaccc gaggatctgg gtaaggatta ctttccattg
     4261 aacgtccatt acgttctccg agcggatcgc cgaacgcgag ccgcctcgat ggcgccggga
     4321 gggctcacac ccgccccggc gtcgatcaaa gcttgccctg cagcagcagg acggtcagca
     4381 gcgcgtagat cgtcagagcc tcgatgaacg cggcaccgat gaccatggcg gtctgaatgc
     4441 gggggatggc ttccggctga cgacccatgg cttccatggc gccacccacg gccttgccga
     4501 ggccgatgcc ggagccgagg gccgcgagac cgacgcccag cggcaggccg agaccgagca
     4561 gattcatgac ttcctccttc tcctagtgat gcgccgccgc gtgcccctca ccgtgaccat
     4621 gatcgtgatc gtggtcgtga tggggcagca tcagcgcgaa atagatggtg ctgagcaacg
     4681 tgaagaccag cgcctggatg gtgcaggtca ggagtcccag gaacatgaag gggacctgga
     4741 acggaatgcc gacgggggcc attgggagga acgagagcgc cccgatgcca aggccgatga
     4801 agacggcgat cagcgtttcc tcgccgaaga tgttgccgaa gagtcgaagc gagagggaga
     4861 gcggcttggc cagctcgccg atcacgtgca gcgggaacat cagcggcacc atcccccatc
     4921 cgatcggatc cttgggctcg cccgccaggt ggtagaggta gccgacgatc ccgttccggc
     4981 gcaggcccgt ccagtgcacg tacaggaaga cgcagatggc cagcgccacc gtcgtgttga
     5041 acgacgaggt gggcgacttg aacaatggca ccaggccgat gaggttgctg acccagatgt
     5101 agaggaagag cgtgccgagg aacggcagga agtgccgccc ttcgagcccc aggatcccct
     5161 ggatgaattc gtcgaagccg ccgatgatca gctccaccag gttctggtgc ttgccgggga
     5221 tcatctcgcg cttggaggcg cccgccacca gcacgcccat gatcaggaag atgacgacgg
     5281 ccgagatgat cggcatctgg aacatcgcca gcgtcttgag ggccgggctg ttgtgatctt
     5341 cctcaccttc gtgaggcttc acatacccca tctgcttcag gatgaggtcg acgaacgagg
     5401 agttctcggc ggtgtgaaag gcttcctgca ccggatcgtg ctcgacagtg tgtccgccgg
     5461 cggcatgggc atccgtggcg tgggcgtcgg ttgcaggagc gtcggcgccg tgagcctcag
     5521 tcgcatgcgc ctcggttccg tgcgcatccg ccggggtcgc gtgctcatcg gcgctgtgcg
     5581 cctcggtggc atgcgtttcc gtcgtggccg ccgcgccgtg cccatgctcc tgggtcagtg
     5641 caatgccggc cggcaacgcc agcatggcga gaacggccag ggcgtatccg acaggagatg
     5701 acagtcgctt catcgatcct ctcctccggc catcggccgc gagaaccagg ccgaatccat
     5761 gagcaggcgg ccgagggact tgagcaggat gaccgccagc accagcgaga ggccggcgaa
     5821 cgtggcggcg ggatcggggt gccgcttcaa catccagacg gcgcccgttc cgtagaggag
     5881 cggaaacttg acgaggaagg ccgagaccag ggcttccatc ctgaggccgc ccggtcgggt
     5941 ggcttccacg atcaccttct ggagcatctt gagattgagc gttccccaga gggccgcaaa
     6001 accgaaatcg acaccccggt cgacgccgaa gtaccagccg gccaccaaag ccaggccgac
     6061 actcaacatc aaggatgtct tgacgatacg tccgaggaac tccggtccca tttcgtttgc
     6121 accttttttc acaagcgaac tcaaaaaaaa ggaagggcga ctcagtccgc gtcgtcccgt
     6181 cgcgtctctg cttccgactc cgccgtggcc ttcttcaaga tggcgatgac ttcacggatg
     6241 ccggcgacca tgccgatggc gcagaaaatc cagacccaga aactggtctt gaaccagtga
     6301 tcgagtttca gtccgatgag ataaccgacg acgggcgcgg ccagaagcag caaaggtatc
     6361 gtcgtcagga gccccacctg ccggagcgag ctgtagcgtt gccggttgcc gttgctcatc
     6421 gacgacgagt gtagatcggc accgtcaacg aaccaaccca ggcaggggcc ggcaggttaa
     6481 ccgagccggc gatttgcgtc aacggatttg gttcttcgag agggctttgg tccggcaggg
     6541 cgagccaagt ctgtaattac ttggccataa atggcttaga cggcctgatc caggccgtcg
     6601 acgtcctcgt cgagggaaag gatggcctcg atgaccaccg ggtcgtaccg gcgccccgac
     6661 tccgccatga gctcgcgccg ggcatcgttt tccgccatcg gcaggcggta cgggcgcggg
     6721 ctggtcatgg catcgaggct gtcggccgcg ccgaggattc gggccggcag agggatggcg
     6781 tctttcttga gcccgtgggg atagccatct cccgaccatc gctcgcgatg gtggaggatg
     6841 gccgactgca cgctcatcgg ccaccgggct tcggccgcga tccgcgcggc atcgccgggg
     6901 tggcgcctca cgcgttccca gtcttcctcg gaaagcgggc ccgggcggac gaggatgtcg
     6961 gtgtcgttca ggagccggcc aaggtcgtgg agctgtcccg cggcccgcag cacctcgagc
     7021 tcgtcgttgg aaagctgaag ccggcggccg gccgccagcg ccatctgggt gacgcgcctc
     7081 gaatgaccgg ccaggtcggg gtaacgcccc tccaaccggt cgaccagcgc gcccattgtg
     7141 tcaacggcca cgtcgcgctc gtggcggcgc aaccgcgccg cttcgaggag atgggccacc
     7201 gacccggcca ggaggccgat cgcctcgaga tcggcgccgg actcgccccg ggcatcctct
     7261 cccatggcga gaaggccgac cagctcgccc cgaaggacga ggggcgcggc gatggagaaa
     7321 ccggcggccg acaacgtgcc cgcctcgaaa gaggtgccgg gaagccggtc gaactcggtc
     7381 aggcggaccg gtccgccggt cgaggccagg atgccgagaa actccccgtc ggtcggcagg
     7441 cggagcccgc tgacctggtc gccggcgatc ccctgccagc gggccgggct caggcacgaa
     7501 agctggccat cctcggggcg gaagaggcag acgccggccg aatgggccag caaggccgcc
     7561 tgggttaggc aggtgcgttc caggtcggcc tcgtcgtcgg cgggatcgag ccggcggttg
     7621 aagtcgagga gagtctgcag tccccccttc tgcacccgca cccgctgccg cagcgtttcg
     7681 agctcctgct gcgtgcggcg gagaatcccg tcgaggtcgt catgcgcgcg gctgagcgcg
     7741 cgcgagtcgg ccaggcgatt gagcgattgg cgcacgcgga gcagcagctc cggcggcgag
     7801 aaagggcgca ccaggaagtc ctcggcgccg atggtgatcc cctggcgggc cgtttccggt
     7861 ggcgagagat cggaaatgac gatcatcggt acccggcgtc ccgagcgctt cccctggagc
     7921 atctcgacga agttcacccc ttcacgggcc accatgccga ggtcgatcag ggagagctcg
     7981 ggagaggtgc ggcgatgatg gctcagggct tcttcggcgt tgaccgccac gcgcacttcg
     8041 tagccggcgc tggccagggt cagttgcagg agttcgcaga ggcgggggtc gccatgcgcg
     8101 acgaggatcg tctcgggaga gcgtggtttc gagttcccgt tcgtgccgtt ggtgccgctc
     8161 aattcagatc tcctggccgt tctccccgtg ccccggttcg acgaagtccg cctccgtcag
     8221 gcgttccacc ggcgccccgc ccatccggag ccgcacggcg gacaactccc tctccacctg
     8281 ccccaacgcc ttgcgcgccc gactcacgag gcggcgccgc tcgaggaaca ccgcggccgg
     8341 cgcgccatcg gccgtgacga tggcctcg
//
LOCUS       DASVOD010000285         7305 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_71935, whole genome shotgun sequence.
ACCESSION   DASVOD010000285 DASVOD010000000
VERSION     DASVOD010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71935"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VF720_06340"
     CDS             complement(<1..727)
                     /locus_tag="VF720_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEX7879010.1"
                     /translation="MQVTVDNFRRAESDTYFARFAKDGGFGKLSHERELAAIDRQTVI
                     RLNRDTLYSFGVFDLDASPVTVILPDAGKRFLAVQVISQDHYATDVFYAPVTTKLTRE
                     SIGTRYVCLAVRTFVNPNDPADVQAVHKLQDAIRVEQSASGTFEPTDWDQDSLTKIRE
                     ALLGLAAANGEMDSSRMFGRKEDVDPVQHLMGPAAGWGGNPRTTAIYSGIVPKENDGK
                     TPYRLTLKDVPVDGFWSVSVYNKG"
     gene            1244..2944
                     /locus_tag="VF720_06345"
     CDS             1244..2944
                     /locus_tag="VF720_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7879011.1"
                     /translation="MFLSLPRWRRNGLWVAFLAVIFSLAVMLTLQYRTLKRLQDVSQA
                     AQAATLNNYLTSIVTEVRYFYLTRAETVLNLPPTAVAGHDSVEIRRHWRRADLSGVRR
                     LFLLEYGEATAPQLVFYDPGVGDFVPALDLRESQSIMRGAIQWVTGASAKGEPKDAFR
                     LRVDERDADFRQIMSPILDDSGRTIGMAGMVLDEDFVKRVLLPVAIQRALPNYFGDAS
                     RNDLMVAIKDADGRTLMGSRKADVSGTPVRQAFPFVFQDWTLEIQSAKPAATQLLEAN
                     FAVWVTLSLLVAGVLAVAIALALSAAARTMKLSEMKTDFVSNVSHELRTPVASIRVFG
                     ELLRRGRVQSPEKVQEYGATIETEARRLSDLIDNVLDFARIESGQKAYRPVRGDLVPM
                     VTAVVDAFRVRLLESGHEIVVQMPQNTLPPVEIDPEALSLALNNLLDNAVKYSGDSRR
                     VFLRLLSDSGAAVVEVEDEGIGIPEDEQAKIFERFHRVGTGSVHDVKGSGLGLAIVHH
                     IMKAHHGRVVVDSAPGEGSTFRLIFPAARGEPAAGANTEILTARRTPVVEPDPRVERG
                     "
     gene            2953..3633
                     /locus_tag="VF720_06350"
     CDS             2953..3633
                     /locus_tag="VF720_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7879012.1"
                     /translation="MPRVLIVEDDASMAVALKDGFEYEGYAVSVAKDGVAGLKMASEA
                     TPDLMILDVMLPRMSGLDVCRQIRSDGHKLPIIMLTSRGQEIDKVLGLKLGADDYITK
                     PFGFLELMARVEALLRRSVGRNEAAESFQFGKVHVNFKKSEVRRNGESVDLSPREFRL
                     LKYLIEHRGEVITRDQLMESVWDYDNAPLTRTVDMHVAKLRRKIEERPNEPSWLVTVH
                     RVGYKFTG"
     gene            complement(3708..5009)
                     /locus_tag="VF720_06355"
     CDS             complement(3708..5009)
                     /locus_tag="VF720_06355"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEX7879013.1"
                     /translation="MRNYRPALSQSFRHFVTAALAALSVTSVAAAPYAPASATPVTPD
                     PLVAPAVNNVPVSITVDEVPGWRQGYTLFVLPGQTITLATRMPDGSAYRWSGDGEFVL
                     PGVARSRAATSGGAGTILPAGSPVVWKAPKGVGGSLLVLRTATADGWVESARIDVQVM
                     APFEALADGLLAGYRIGRYPAQPKKKTVDQNLADYTHPVGFLKLPEKSAAPIKVSPRF
                     FLHDFVCKQDGEGDRYVALRPELLQMLEAIVDRVESEGFRCSLLDHDGGTVQLASFNE
                     GSSNIAPSAPGRPLLIMSGYRTPAYNRSLGNVKLSRHQFGDASDIIVDSDGDQVMDDL
                     NHDGRLNGQDAITLASWINEIWQTEQFAGRPGGLGVYNAEGEHGPFVHVDMRGVKARW
                     SGNGLRWAEAEDKDAHDTPEPGATAAAPKSTKTKARKNVVR"
     gene            5286..6908
                     /locus_tag="VF720_06360"
     CDS             5286..6908
                     /locus_tag="VF720_06360"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7879014.1"
                     /translation="MNPAKIDAALVSRELGSRLVSISRSGLLSPLRTAEVYRRNGGKP
                     VFLGSASGVTHADRVVTAIKDAGAQGINPARYRPEELDRAILATPEMSDSWWERLSSL
                     GKTEAQAGALANADLVLTDRYLAMAADLKGITDKDSAAVDSLLRVTADRTPPIEDWAP
                     EHEQYRRLIDALAVYRQVAEAGGWPRIDAGEKLVKGNRGTRVGQLRARLDAEARLLKQ
                     FGGHSADSLYGEPVADPLLFDATLKKRVADFQARASLENDGVAGKGTLAELNLSAAER
                     VRQIERTLERWRRGPRVEPDRRLEVIVPGMWLDLYEGGKPVLTLKTVVGSGRESAEWK
                     GETSIAHTPELIDEIERIEINPKWHIPEIIIRTELLAKEKREPGFLDKGGYEWYEPGT
                     KKFGPASELPDSIWTGRKRLYLRQRSGDANALGKMKFLFPNPYAVYLHDTPDKKYFNR
                     TRRAFSHGCVRVQNPTVLAESLLAHTTTPPPKPLGQMLKTSTLYVAPITPHVPIHLLY
                     RTTWVEENGRVRFLSDLYGWDAAFDSLTAEAK"
     gene            6905..>7305
                     /locus_tag="VF720_06365"
     CDS             6905..>7305
                     /locus_tag="VF720_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879015.1"
                     /translation="MTSAVRRRYAAPEHFVARRVTAAEHLDDPGTPADDRAASYRDLE
                     RFETWPWQFGPFLAGVWQVLGPVPPRAVSILEMGAGTGHTGRRIKAALAARGIEVTLH
                     LTDRDPGVVADTHGVRVSRLDWLADPIPGADV"
BASE COUNT         1279 a         2351 c         2420 g         1255 t
ORIGIN      
        1 cgcccttgtt gtacacgctg accgaccaga agccgtcgac cggcacgtcc ttgagcgtga
       61 ggcggtacgg cgtcttgccg tcgttctcct tcggcacgat gccgctgtag atcgcggtcg
      121 tccgcggatt gccgccccat ccggccgcag gtcccatgag gtgctgaacg ggatcgacat
      181 cttccttccg cccgaacatg cgcgaggaat ccatttcccc gttggcggcg gcgagaccga
      241 gaagtgcctc acggatcttc gtcagcgaat cctggtccca gtctgtcggc tcgaaggtgc
      301 cggatgccga ttgttccacg cggatggcat cctggagctt gtgcaccgcc tggacgtcgg
      361 ccgggtcgtt cgggttcacg aacgtccgaa cggccaggca cacgtagcgg gtgccgatcg
      421 attccctcgt gagcttggtg gtgaccggtg cgtagaacac gtcggtcgcg tagtgatcct
      481 ggctgatgac ctggacggcc aggaaccgct tgcccgcgtc cgggaggatc accgtgaccg
      541 gcgaggcgtc gagatcgaac acgccgaacg agtagagcgt gtcgcgattg agccggatga
      601 ccgtctggcg gtcgatcgcg gcgagctccc gctcgtggct cagtttgccg aagcctccat
      661 ccttcgcgaa ccgcgcgaaa taggtgtcgg attcggcgcg gcggaagttg tcgaccgtta
      721 cctgcatggg atgtgtctcc tccggttggc gcgcgagcag acatttcgat tgggcatgat
      781 attgccggcg agggtgccgc tgaaagccgg agagcgggtg gcggtgagag aggtcagggg
      841 cggagccggg gagggtcggg atggcgatcg gggaggaaaa accgcccccc aaaaaaaagg
      901 cgggaggtcg gcgggctgcg gtcgccaacc tcccggccga cgctccggga cgaatcccgg
      961 atcgcgctcg ggtcaggggt cacctgagat aaactattgc ccctgaatgc tgcagggagg
     1021 gtttgcctgg gcccatcacc cggcttgccc tccctacctc ggacaaactg ctctcggttt
     1081 tgtgtcgccg ctcggtgtgc ctacctactg gcgacaaaca gagattagct ttcggtcatg
     1141 taaatgtcgc acccgataag gtaaagagtt gtcaacggtg taaccgactt gtaaacacga
     1201 acccatcgca ctccacaatc ccttacccct gttatactta gatatgttcc tctccctgcc
     1261 ccgctggcgt cggaatggcc tctgggtggc cttcctggcc gtgatttttt ccctggccgt
     1321 catgctgacc cttcaatatc gcacgctgaa acggctccag gacgtttccc aggcggcgca
     1381 ggcggccacc ctcaacaact accttacctc gatcgtgacc gaggtccgct acttctatct
     1441 cacacgggcc gagacggtcc tgaacctgcc gccgactgcg gtggccggcc atgattccgt
     1501 ggaaatccgt cggcattggc gccgggccga cttgagcggg gtgcgcaggc tctttctgct
     1561 cgagtacgga gaggccaccg ccccgcagct tgttttctat gatcccgggg tcggagattt
     1621 cgtcccggcc ctcgacctcc gcgaatccca gtcgatcatg cgcggagcca ttcaatgggt
     1681 gacgggggcc tccgcgaagg gggagccgaa agatgccttt cgcctgcgcg tggacgagcg
     1741 cgacgcggac ttccggcaga tcatgagccc gatcctcgac gattccggcc gcacgatcgg
     1801 aatggccggg atggtcctgg acgaggattt cgtgaaacgg gtgctcctcc ccgtcgccat
     1861 ccagcgggcc cttccgaact acttcggcga tgcctcgcgc aacgacctga tggtggcgat
     1921 caaggacgcc gacggtcgca ccttgatggg gagccgcaag gcggacgtct ccggcactcc
     1981 cgtccgccag gcgtttccct tcgtcttcca ggactggaca ctggaaatcc agagcgccaa
     2041 gccggcggcc acccagctcc tcgaggccaa tttcgccgtc tgggtgacgc tctcgctcct
     2101 cgtagccgga gtcctcgccg tggccatcgc gctggcgctc tccgcggcgg cccgcaccat
     2161 gaagctctcc gagatgaaga ccgatttcgt ctccaatgtc tctcacgaac tgcggacccc
     2221 ggtggcgtcg atccgggtgt tcggggagct gctccggcgc gggcgggtcc aatctccgga
     2281 gaaggtgcag gagtatgggg ctaccatcga gaccgaggcc cggcgcctct cggacctgat
     2341 cgataacgtc ctcgatttcg cccggatcga atcgggccag aaggcctatc ggccggtgcg
     2401 tggtgacctg gtacccatgg tgaccgccgt ggtcgatgcc tttcgtgtgc gattgctcga
     2461 atccggacac gagatcgtgg ttcaaatgcc ccagaacacc cttcctccgg tggagattga
     2521 tccagaggca ttgtcattgg cgttgaacaa tctgctcgac aatgccgtca aatactccgg
     2581 cgattcccgg agggtctttt tgcgcctgct ctccgattcc ggtgccgccg tcgtcgaagt
     2641 cgaggacgag ggcatcggga tccccgaaga tgagcaggcc aagatcttcg agagattcca
     2701 ccgggttggg acgggttcgg tgcacgacgt caagggaagc gggcttggcc tggccatcgt
     2761 gcatcacatc atgaaggcac accatgggcg ggttgtcgtg gacagcgcgc cgggagaagg
     2821 cagcaccttc cgcctgatct tcccggccgc gcgcggcgaa cccgcagccg gggcgaacac
     2881 ggagatcctg accgcccgcc gtaccccggt ggtggagccc gacccgaggg tggaacgagg
     2941 atgacaaagg ccatgccccg agttctgatc gtggaagatg acgcctcgat ggctgtcgct
     3001 ctgaaagatg gcttcgagta cgaaggttac gccgtttccg tggccaagga cggcgttgct
     3061 ggattgaaga tggccagcga ggccacgccc gacctcatga tcctggacgt catgctcccc
     3121 aggatgagcg ggctcgatgt ctgccggcag atccgctccg acggccacaa gctgccgatc
     3181 atcatgctca cttcacgcgg gcaggagatc gacaaggtgc tggggctcaa gctcggtgcc
     3241 gacgactaca tcaccaagcc gttcggtttc ctggagctca tggcccgcgt cgaagcgctt
     3301 ctccgccgca gcgtggggcg caacgaggct gccgagtcgt tccagttcgg caaggtgcac
     3361 gtgaacttca agaagagcga ggtacgccgg aacggcgaat cggtggacct ctcgccgcgc
     3421 gaattccgtc tcctcaagta cctgatcgag catcggggcg aggtgatcac ccgggaccag
     3481 ctcatggaaa gcgtgtggga ctacgacaac gcgcccctca cccgcaccgt ggacatgcac
     3541 gtggccaagc tccgccgaaa gatcgaggag cgccccaacg agccctcctg gctcgtgacc
     3601 gtgcaccggg tggggtacaa gttcacgggg tgacggggaa atccaggacg aaatgaaaaa
     3661 ggccgccggc gatgccggcg gcctttccat tgttgttgca aactgtgtca gcggacgacg
     3721 ttcttccggg ccttcgtctt cgtgctcttc ggcgccgcgg ccgtcgcgcc gggctccggg
     3781 gtgtcgtggg cgtccttgtc ctcggcctcg gcccacctca aaccgttgcc gctccagcgg
     3841 gccttcacgc cccgcatgtc gacgtggacg aacggtccgt gctccccctc ggcgttgtac
     3901 acgccgagcc cgcccggccg accggcgaat tgctccgtct gccagatctc gttgatccag
     3961 gaggccaggg tgatggcgtc ctggccgttg aggcggccgt catgattcag gtcgtccatg
     4021 acctggtcgc cgtcggagtc gacgatgatg tcggaggcat ccccgaactg gtggcgggaa
     4081 agcttcacgt tgccgagcga tcggttgtag gcgggcgtgc ggtaaccgct catgatgagg
     4141 agcggccgtc cgggagccga cggggcgatg ttcgagctgc cttcgttgaa actggccagc
     4201 tgcacggtgc cgccgtcgtg atcgagcagt gagcagcgga agccttccga ctccacgcgg
     4261 tcgacaatgg cttcgagcat ctggagcagc tcgggccgaa gggccacgta acgatccccc
     4321 tcgccgtcct gcttgcagac gaaatcgtgg aggaagaagc ggggcgacac cttgatcggg
     4381 gccgcggact tctcgggcag cttgaggaag ccgaccgggt gcgtgtagtc ggcgaggttc
     4441 tggtcgacgg tcttcttctt cggctgggcc gggtagcggc cgatgcggta gccggcgagc
     4501 aggccgtcgg ccagtgcttc gaacggggcc atgacctgga cgtcgatgcg ggccgactcg
     4561 acccaaccat cggccgtggc cgtgcggagg accaggagcg agccacccac acccttcggg
     4621 gccttccaga cgaccggcga gccggccggc aggatcgtgc ccgctccgcc gctcgtggcg
     4681 gctcttgacc gcgccacacc cggcaggacg aactccccgt cgcccgacca gcggtaagcg
     4741 gaaccgtcgg gcatacgggt tgcaagggtg atggtctggc cgggaagaac gaacagggtg
     4801 tacccctgac gccatcccgg gacttcgtcc acggtaatgc tgacgggaac attgttcacg
     4861 gccggggcaa cgagcgggtc gggtgtcacc ggtgtcgcgc tcgccggcgc atagggtgcg
     4921 gcggcgaccg aggtgaccga cagagcagcc agggccgcgg tcacgaagtg acgaaaggac
     4981 tgggacaggg caggacgata attccgcatt tctctccgtc tcaattcttg ggggttacga
     5041 aggggcggcc cggctgatga tcgacgacgc ggacgtcgag cgtgccgtga gaccactccc
     5101 agaatacgtt gttccaatag aaatcggcca ccctaaagtt gtcctgaagc gagagctgtg
     5161 actgtctcaa aaaaagggta aactcttccc ccatgatccg tctccgccga ttggcaataa
     5221 cactactcct tacaatggtt tacgcgaatt caggggccct cgcgcaaggg cccgtgtccg
     5281 agcgcgtgaa cccggcgaaa atcgacgccg ctctcgtgtc gagggagtta ggttcccgcc
     5341 tggtttcaat ctcccgctcc gggctcctct cgccccttcg gacggccgag gtttaccgcc
     5401 ggaacggcgg caagccggta ttcctgggct cggcgtccgg cgtgacgcat gccgacagag
     5461 tcgtgacggc catcaaggac gccggcgcac agggaatcaa cccggcccgg tacaggccgg
     5521 aggagttgga tcgcgccatc ctcgccactc ccgaaatgtc ggactcgtgg tgggagcgcc
     5581 tgtcgagcct gggcaagacg gaggcgcagg ccggcgcgct ggccaatgcc gacctggtgc
     5641 tcaccgatcg ctacctggcg atggcagccg acctgaaggg gatcaccgac aaggactccg
     5701 ccgcggtcga ttcgctgctc cgcgtcacgg ccgacaggac gcccccgatc gaagactggg
     5761 cgcccgagca cgaacagtat cggcggctca tcgacgcgct ggccgtctat cggcaggtgg
     5821 ccgaagcggg aggctggccg cgcatcgacg ccggggaaaa gctcgtgaag ggaaaccgcg
     5881 gcacgcgcgt cgggcagctc cgggcccggc tcgatgcgga agcacggctc ctgaagcagt
     5941 tcggcggcca ctccgcggac tcgctgtacg gcgagccggt ggccgatccg ttgctcttcg
     6001 acgccaccct caagaagcgg gtggccgact tccaggcacg cgcctcgctc gagaacgatg
     6061 gcgtggccgg caagggaacg ctggccgagc tgaacctgag cgccgccgag cgggtccgcc
     6121 agatcgagcg cacgctcgag cgctggcggc gtggcccgcg ggtcgaaccg gatcgtcgcc
     6181 tcgaggtgat cgtgcccggc atgtggctcg acctgtacga aggggggaag ccggtactca
     6241 cgctgaagac ggtggtgggg tccggtcgcg aatcggccga gtggaaagga gaaacctcca
     6301 ttgcccacac tccggaactg atcgacgaga tcgagcggat cgagatcaac cccaagtggc
     6361 acattccgga gatcatcatc cgcacggaac tgctggccaa ggagaagcgg gagccgggat
     6421 tcctcgacaa gggtggctac gagtggtacg agccgggaac gaagaaattc gggccggcct
     6481 cggagctccc cgattccatc tggaccggca ggaaacgcct ctacctgcgc cagcgctcgg
     6541 gagatgccaa cgccctcggc aagatgaaat tcctctttcc gaatccgtac gccgtctacc
     6601 tgcacgacac cccggacaag aagtacttca accggacgcg ccgggccttc agccacggct
     6661 gcgtgcgcgt gcagaacccc acggtgctgg ccgagagcct gctggcgcat accaccacgc
     6721 cgccacccaa gccgctgggc cagatgctca agacgtccac gctctacgtg gcgccgatca
     6781 cgccgcatgt gccgatccat ctcctctacc ggaccacctg ggtggaggag aacggccgcg
     6841 tgcgcttcct cagcgacctc tacggctggg acgccgcctt cgactcgctg accgccgaag
     6901 caaagtgacg tcggccgtgc gacggcgtta cgcggcgccg gagcacttcg tggcccgccg
     6961 ggtgacggcc gccgagcatc tcgacgaccc cggcaccccg gccgacgacc gggccgccag
     7021 ctaccgtgac ctcgagcgat tcgaaacgtg gccctggcag ttcggcccgt tcctcgcggg
     7081 cgtgtggcag gtgctcggcc cggtgccccc acgcgcggtg tcgatcctcg agatgggcgc
     7141 cggcaccggt cacaccgggc gacggatcaa ggcggcgctc gccgcgcgcg gcatcgaggt
     7201 gacgcttcat ctcaccgacc gggatccagg ggttgtggcc gacacgcacg gcgtgcgggt
     7261 gagccgcctc gactggctgg ccgatccgat ccccggggcc gatgt
//
LOCUS       DASVOD010000286         2694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_384218, whole genome shotgun sequence.
ACCESSION   DASVOD010000286 DASVOD010000000
VERSION     DASVOD010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384218"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="VF720_06370"
     CDS             complement(<1..808)
                     /locus_tag="VF720_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7879016.1"
                     /translation="MKSDLERLMAEKGLDALVVLGAAAGNPSLYYVVDGAGLGNAIWI
                     KRKGHEPHLVYNPMERDQARATGIDGSTFPENDWSRFLEEHGGEGPETTAAFLAHLLK
                     KNGASGTVGVYGTGEVARLYPMIRKLGETPGITIHEETNGRSLLEAARITKSEAEVAR
                     IRKVALSCFDAMDGIKAIIKAGKLMNGRLHDRAGRALTIGDLRAAVRRAWAQHGVIED
                     HDSVVAMGKDGTAPHNRGTDTDVLEAGKSIVVDIFPREGGGGYFFDMTRTF"
     gene            complement(852..>2694)
                     /locus_tag="VF720_06375"
     CDS             complement(852..>2694)
                     /locus_tag="VF720_06375"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX7879017.1"
                     /translation="MSFLFFWRIAGSRSGAAVGAALLAVSPIWWAQSTIIEVYPLHLA
                     FMATLFYLALSPGGWPRRLLLLAWIGGLALVHHRMAALAAPSLAVLAGAALWRHRPIN
                     WRYVAMAAPLVLAPLTLYLVLLIRSRLDPALDWGNPERWSQLGWVARGEQYRFRMFHE
                     PLSAFAERARVAAFDVMPAQFGATALGLSLVGLLAFRKRGGAAAWWAMVVYLLVSLVA
                     YAGYDIPDPDAYTLPMTFVLAGFAALGAAWLLERRWFGGRWGGALVVAALLMMPVARL
                     PALWRTTTLHDDKGAEDYARDALALLPERAVVLSDGDGRSFSLWYRQVVNGDWDKAIV
                     YRLLLVWPWYHENLRVRHPDLPLPPVTRSVLENGKQFVAMNLAAGRPVYTTTVDDWLS
                     DGWSAAPEGRLFRVLAPRRSFTPPGTEPRWRSLDLAAIANCDYRLDPFTAGSKDSTGL
                     IFGLGGGRQYWGSLPFVLMRPRSFTGNWSAFTTGGLPAVTARLPLEAAPTSMVVLAVD
                     AWSGPASLRLGEVRVEYETGPADTVTVQSYANVWDFHAESQEVRLPKDLLVWFSTLDQ
                     SLTALPVTVDPARKPVALVLTGEGPLTGDDRHAGYTVFAATQVLAGP"
BASE COUNT          457 a          941 c          921 g          375 t
ORIGIN      
        1 agaaggtgcg tgtcatgtcg aagaagtagc cgccgccccc ctcgcgcggg aagatgtcga
       61 ccacgatcga cttgccggct tccagcacgt cggtgtcggt gccgcggttg tgcggggcgg
      121 tgccgtcctt ccccatggcc acgaccgagt cgtggtcctc gatgacgccg tgctgcgccc
      181 acgcacgccg gacagcggca cgcaggtcgc cgatcgtgag cgcgcgtccg gcccggtcgt
      241 gcagccggcc gttcatgagt ttgcccgcct tgatgatggc cttgatgccg tccatggcgt
      301 cgaagcagga gagcgccacc ttgcggatgc gggccacctc ggcttcggac ttggtgatcc
      361 gggccgcctc gaggagcgag cgtccgttcg tctcttcgtg gatggtgatg ccgggcgtct
      421 cgccgagctt ccggatcatc gggtagagcc gggccacctc gcccgtgccg tacacgccca
      481 cggtgcccga ggcgccgttc tttttaagga ggtgggccag gaaggccgcg gtggtttcgg
      541 gcccctcgcc gccgtgctcc tcgaggaagc ggctccagtc gttctctggg aaggtggagc
      601 cgtcgatgcc ggtggcccgc gcctggtcgc gctccatggg gttgtacacg aggtgcggct
      661 cgtgtccctt gcgcttgatc cagatggcgt taccgaggcc ggcgccatcc accacgtaat
      721 aaaggctcgg attgcccgcg gcggcaccga gcacgacgag ggcatcgagc cccttttcag
      781 ccatgagacg ttcgagatcg cttttcacgg gtcctccgga cgcaggttct atcaggcggg
      841 gaggaatcgt atcacggtcc ggccagcacc tgagtggccg caaagacggt gtatcccgcg
      901 tggcgatcgt cgccggtcag cgggccttcg cccgtcagga caagcgccac gggcttccgg
      961 gccgggtcca ccgtcacggg aagcgccgtc aacgactggt cgagcgtgga gaaccagacc
     1021 aggaggtcct tgggcaggcg aacttcctgc gattcggcgt gaaaatccca gacgttggca
     1081 tacgattgca cggtcaccgt gtcggccggg ccggtttcgt actccacccg cacctccccc
     1141 agccggagcg aagcggggcc gctccaggca tcgaccgcca gcaccaccat cgaggtgggc
     1201 gcggcctcga gcggcagccg ggccgtgaca gctggcaacc cgccggtggt gaaggctgac
     1261 cagttgcccg tgaacgagcg cggacgcatc agcacgaacg gcaggcttcc ccagtactgg
     1321 cggcccccgc cgagcccgaa gatgaggccg gtcgagtcct tcgagccggc ggtgaagggg
     1381 tcgaggcgat aatcgcaatt ggcgatggcg gccaggtcga gcgaacgcca gcgcggttcc
     1441 gtgcccggcg gtgtgaacga ccggcgcggc gcgagcacgc ggaacaaccg cccctcgggc
     1501 gccgccgacc agccgtccga gagccaatcg tccaccgtcg tggtgtacac gggacggccg
     1561 gcggccaggt tcatcgccac gaactgcttc ccgttctcga gcacgcttcg cgtgaccggc
     1621 ggcagcggga gatcgggatg gcgcacacgc aggttttcgt gataccaggg ccacacgaga
     1681 agcagtcggt agacgatggc cttgtcccag tcgccgttca cgacctgccg gtaccagagg
     1741 ctgaagctcc ggccatcgcc gtcggaaagc acgacggccc gctcgggaag cagcgccagc
     1801 gcatcgcgcg cgtagtcctc ggcgcccttg tcgtcgtgga gcgtggtcgt gcgccacagg
     1861 gcgggcagcc tggcgaccgg catcatgagg agcgcggcca cgaccagcgc acccccccat
     1921 cgcccgccga accagcggcg ttcgaggagc cacgccgcgc cgagcgccgc gaagcccgcc
     1981 aggacgaagg tcatcggcag cgtgtaggca tccggatcgg ggatgtcgta gccggcgtac
     2041 gccaccagcg acacgaggag atagaccacc atcgcccacc acgcggccgc gccgccacgc
     2101 ttccggaatg ccagcaggcc aaccagcgaa agcccgagcg ccgtggcgcc gaactgggcc
     2161 ggcatcacgt cgaacgccgc cacgcgcgcc cgctcggcga aggccgacaa cggctcgtgg
     2221 aacatgcgga aacgatactg ctcgccgcgc gccacccagc cgagctgact ccacctttcc
     2281 gggttccccc agtcgagcgc cgggtcgagg cgcgatcgga tcaacaacac caggtagagc
     2341 gtgagcggcg cgagcaccag cggcgccgcc atcgccacgt accgccagtt gatcgggcga
     2401 tgacgccaca gcgccgcacc tgccagtacc gccaggctcg gcgccgccag cgccgccatc
     2461 cggtgatgca ccagcgccag cccgccgatc cacgccagca gcaggagtct ccgcggccac
     2521 cctcccggcg agagcgccag gtagaagagc gtggccatga acgccaggtg gagcgggtac
     2581 acctcgatga tcgtcgactg cgcccaccag atcggagaga cggccaacaa tgccgcgccg
     2641 acggcggcgc cgctccgcga acccgcgatc cgccagaaga agaggaacga catt
//
LOCUS       DASVOD010000287        12306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_489445, whole genome shotgun sequence.
ACCESSION   DASVOD010000287 DASVOD010000000
VERSION     DASVOD010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12306
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489445"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="VF720_06380"
     CDS             <1..709
                     /locus_tag="VF720_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879018.1"
                     /translation="MAYRVGLDPSGVMNSTWMMPAGRSGIPCAFVVDTRGDLAWIGHP
                     GDPEMTGVIDDVVAGTWDVAAYKVRKAKIREAEAKLTTAAKNKDAAGMIAGLDDLMVV
                     DPTRYDQAAIKKFELLLFGVHDYQQGYAWAGQLVDVIFKDRADMLNAIAWAIVDGKGL
                     EERNLDIALRAATRAEDLTDGGDMQVLDTLARVHFEKGNVKQAVTVQEQAVKLAIDPK
                     RKGEAQAKLDKYKAAQG"
     gene            868..1419
                     /locus_tag="VF720_06385"
     CDS             868..1419
                     /locus_tag="VF720_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879019.1"
                     /translation="MFDPTFGTGRATRIVLLALVAGTMALSVQAFRSRPLAASETTPL
                     TTCDLKHPIDAKLVPLDPIARGSLVRVALDVTSSVPVSGLEARIQSTGGARLVGKRRA
                     ELGTLDAGDARRTEFDIELPAAGKRFLLQVLVTAEAATGPMNRLVVLNILPDGPADPG
                     QVVTTPTGESIRQYANSARRIER"
     gene            1416..4790
                     /locus_tag="VF720_06390"
     CDS             1416..4790
                     /locus_tag="VF720_06390"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7879020.1"
                     /translation="MNCLERLIARRGSDAAIGGLLILMWVLGLAAFVVLSAFASPADA
                     AFNASGRFLYADKLWDKDGYTGQEQNLPVRHARVEILDIPTLQVIGSGATDNNGNWSV
                     TVTPLLPIVPVNIVARVLTDGRTAGYEIRVVDELERGLGEPAVVGNIHAVLTDPVLNH
                     NQSQPRAFGTYLLQDTDGTGVAQAFNIFDNAVDFFDWMAQPSLLGRRPNAGEYIVYAW
                     SPIGTNEGSNYSGHAILLSSPGQGNDTDAWSDTVILHETGHWFDDFFSRSDNPGGAHS
                     LGDNNANVKLAYGEGVATFHCSKVRKLRATTRTNLVGQPVDRHVSIYGDLTIPPDSMM
                     AGGLSFSYDFETGVAFQPGLPPGGFFLGQRGSANETNVTSAQWDLVDDASTPDETPGI
                     DDDQVGVSDAVCFNIEHNWLPGLPASNVVTIEDYYQGWYAQNGANYLRAGVDDIFLNL
                     SLMEFYADGYEPDGTPAQATAITPLAYTVTPGAHLVISELDLGAQDAVEVFNPSDAAV
                     NMTGWTIQVYVNDDTAPVVSRVYVFPPFTLDPGEAVAVLERGDPLNNGAHHLYGGSTV
                     PNSWNASWNFGLDGACVLNNASQQAVDFVTWRAANGTPNSTPVPSGTAFTGVLDSPAA
                     PFNLARDINGTDTDAATDWTAREGTLASANGSFSRHQTLFDVGDADLIRFDAVAGQRY
                     GFEVRAFYSASDPYVELISPTGTVVGSNDNVDPGITDARIDLFAGSTGTYYLKVRHVG
                     PNTDWAAYNVAAFQRPPSDVLLAPGGITATAANTNNSGDPVHLEWLNGAVYDNVKLYR
                     NGTLLATLPGDVDAYDDAADRGVYTYEVSGVRGLTETARVSTNEFAGLVGCFVSEDFE
                     EGAAPDWVSEANTWGVTPLAEGGVYGYTDSPVGTSRGCHGVLENCSMSTATVLRVPVI
                     LPPGSRLLFDHICITEHCEPVPCDFGAVEISTDEGQNWTELARYDQASDPGWADNAAD
                     PTDWRPADISLNAYTGQTVLVRFRVQTDPLTELDGWYLDNVRINEGDCTIAAVPPAGG
                     IRTELMAPYPNPMTGTARFAFRLAEPAPDVSMSIYDLSGRLVRREALGALDAGDHLWQ
                     WNGQSDSGEGTANGVYFARLETAGQLFTRKVLKVTR"
     gene            5012..6229
                     /locus_tag="VF720_06395"
     CDS             5012..6229
                     /locus_tag="VF720_06395"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879021.1"
                     /translation="MNSAEVAPIRSWPGWSRGRRLAVTVARGALWLAAAHPGQADPTR
                     SHPLPDPLEVAFRVAHEASGLNRPVIEVMAIQPVHVTLMPAQAAPAPGPADWLEVSDS
                     DDPLFIYWPPRLPGAAVRDAWYRRSPVVTPHGPGPLLAGQLPADGSASSTADDEARID
                     DEFEVGEFEGVASDGEPVGLPIRTVLPEPNGGEVWSADSLATVRWQAEGGVDPLFIRL
                     EFSVDDGRSWRLLADSLYNEGSWDWEVPWLEARRARLRVMARDDSGLVGVDAADRSFT
                     IRKPEWIGMTPPPLLALDAGSRLSAGSFRLVVELAAATPVELVILAVTGRLVRRLMDG
                     PMEAGGHVVEWDGRGENARPVASGIYFARLMALGDRLVRRLVLVRGRRRTGRRPGWWG
                     MTLPRYTGDRRPA"
     gene            6296..7357
                     /locus_tag="VF720_06400"
     CDS             6296..7357
                     /locus_tag="VF720_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEX7879022.1"
                     /translation="MPKTEVMSCRPSRPTLSSWLAFASLLMMTTLAIHVSSASAQQVP
                     DTTWTPSGHTPHWSSGSGPRVVIDAAHHNFHTVDGRYRPFADLLRRDGYRVSGSNESF
                     RAATFSGIDILVIANANSARNESDWSLPVTSAFEPDEVDAVRAWVRDGGSLLLIADHM
                     PFPGAAAPMAAAFGISFRNGFAGAGHELSPITFRRGPVGVGCFGGQLATHPIATGLRP
                     GEGVDSLVSFTGSAFRPPDGAEPLLILPSDTISLEPAQAWQFEEQTPRTDVGGWCQGA
                     VLRYGRGRVAVFGEAAMFSAQLQTGSTPPTPMGMNVPRAPQNFRFLLNTMAWLAESDG
                     KGSVNTPSTPGTTRQRAGR"
     gene            7510..8031
                     /locus_tag="VF720_06405"
     CDS             7510..8031
                     /locus_tag="VF720_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7879023.1"
                     /translation="MSQRWETSLYRFIRRMMGNSEDARDVCQEAWAKAYQNIGTLREG
                     AKFRAWIHYIALNLCRDRFRSLKSKAIHSSYEEGGLEVLPEGSGKTAQWATTRHVEQT
                     SLKTLLDDALTALPNEQRTAIVLREYQGFTSEEIAEITGVPAATVRSRIFYGFKALRR
                     ILGERGISPEQSS"
     gene            8054..8851
                     /locus_tag="VF720_06410"
     CDS             8054..8851
                     /locus_tag="VF720_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879024.1"
                     /translation="MKRHEAVDPKIDRMIAALYGELNDAERLEFEKQLANDPALRAEW
                     EEISGTRNLLTGWELEDRVPSFVVVPAVATRPRVAMGWVDVLRERFGNWFSVGGWVVA
                     GAAAAVLLMLAKDIRIEKTPNGIAFNYGGKAPVNHVLPTDPSGSDFGGSPRLVNDGAD
                     GGQYVTKNELDQQSAAILRMMSAYMDDYRGRRDQELGAALRSLYGNLNARQASQYEDL
                     LGKIDANHPGERTEHEFLRPTELGMSHADSLGPLRLSPEDVEVGRNE"
     gene            8844..9584
                     /locus_tag="VF720_06415"
     CDS             8844..9584
                     /locus_tag="VF720_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879025.1"
                     /translation="MNNRPRSFLAGIGLVLSLLVPGLVHHSLAAPAAAQTSEPVRQKR
                     MLRDITVLEKIIDEMLLDSKNLLIYTAGGVTHGLYIDEFGALFSFEASLVDRSDDSPW
                     PEGMDAYTFDTDEHGNVIIRKKKDSKGKGPDTPVPPTPPRPGETPKAPPAPRAWIDRQ
                     AGKQAKLYETGKAEIIDTLIEYGDSMDGLRDNQWLCFAAFLKNSDFFLENRISRLVVK
                     ARMSDLRSYAQGRIDRKAMMAKVVQEEY"
     gene            complement(9617..>12306)
                     /locus_tag="VF720_06420"
     CDS             complement(9617..>12306)
                     /locus_tag="VF720_06420"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX7879026.1"
                     /translation="GHVRVTQGRPTTAGAALPVWYDGPGGLLPAYRVIVEGGLDCRRE
                     VIVDGRTGSIVKDLSLVQTDGAVTGSGPDLANVNRTLGLYQIGSTFFMTDTRKDMFNQ
                     AASTFPNMPSGTITLINAAHGMGDNLTHVTSNNTTTWTNFRNAVSGANFAAQVYDYYR
                     TRHERFSIDGNKGTMWLVVNFQNNFNNAFWNGQFMVFGNGDGNQFSDLAGALDVTAHE
                     MSHGVIENTAGLVYEFQSGALNEHFADAFGAATEFFATPGNANWLLGEDVTTPGTAGD
                     ALRNMQNPAASNVAFGGQQPTKMSEFLNLNSNQDNGGVHINSGIPNRAFYLTCTALDV
                     ARGERIWYRALSQYLMRNSQFVDFRRATIQAAADLFGGGSAEVTAVTNAMNTVEIMDG
                     GMTPPPPPAPDNVGSDFVAVVDAGNDHIVTIDPGFQGQVVDLSGLATGPGGRPTFTDD
                     GQLMAFVGSDGNVYARDADGPVQALSDMGGWWSVAFSPDGSRLAATTVEEDGRIYSFD
                     LVGENNVFYDLTSQNSTSGNVADQVVFADVMEFSLDGEYIVYDALNRSNLGGQMFEYW
                     DINLLRLSDGECFRVFQPLPQGESVGNPALAQNSDNRIAFDYASNDGNVYVIGYDFQS
                     GVSGTITNNQQSTGRPTFSGDDAQVYYQYVTGSDAVIYRVGLDSDGVTGSGNDAVWAS
                     DGYAPVWYTVGTRPTPVALLAFDMTWQDDEITVSWRIGDPDGAVGFRLSAAPARDAAY
                     SDAGSLIPVTAGAAGSYEARVTAPLHAATLWIKLEEISRGGSVREVIRKEAIRIPSDA
                     AGALLLSPAWPNPTAGAASFEFRIPASAGTSRTSLAIFDAAGRRVAGVVDELEITAGT
                     TVVRWDGRDDSGNPVPSGRYWARLTSGTNHAVRPIVIAR"
BASE COUNT         2044 a         4173 c         4052 g         2037 t
ORIGIN      
        1 gatggcttac cgcgtcggcc tcgacccgtc gggcgtcatg aattccacct ggatgatgcc
       61 ggccggtcgg tcgggaatcc cgtgcgcctt cgtcgtcgac accaggggcg atcttgcctg
      121 gatcggccac cccggcgacc cggaaatgac cggtgtcatc gacgacgtcg tggccggcac
      181 gtgggacgtg gccgcgtaca aggtgcgcaa ggccaaaatc cgggaagccg aggccaagct
      241 caccaccgcg gcaaagaaca aggacgccgc ggggatgatt gcgggcctcg atgacctgat
      301 ggtggtcgat cccacccgct acgaccaggc ggccatcaag aaattcgagc ttctcctgtt
      361 cggcgtccat gactaccagc agggatatgc gtgggccggg cagctcgtcg atgtcatctt
      421 caaggatcgc gccgacatgc tgaacgccat cgcctgggcc attgtcgacg gcaaggggct
      481 cgaagagcgc aacctcgaca tcgccctgcg cgccgccacc cgcgccgaag acctcaccga
      541 tggtggcgac atgcaggtgc tcgacaccct tgcccgcgtc cacttcgaga aggggaacgt
      601 gaagcaggcc gttaccgttc aggaacaggc ggtcaagctg gcaatcgatc cgaaacgcaa
      661 gggcgaggcc caggccaagc tggacaagta caaggcggcg cagggctgaa attcatcaag
      721 taattgaaat tatttgactt atagggtcga ggcggccgca atgccgcctc cactcaagcc
      781 ccactcaaca cccccccgac agacttcctc ctgtcagtcc gccatgtcgg cgcactgaga
      841 gccacgctct tcgaggaggt ttgtccgatg ttcgatccga cgtttggaac gggtcgcgcc
      901 acccgcatcg tgctcctggc cctcgtggcc gggacgatgg cgctgtcggt tcaagcgttt
      961 cggtcacggc cgctcgccgc atccgaaacc accccactca ccacctgtga cctgaagcat
     1021 ccgatcgacg cgaagctcgt tccgctcgat ccgatcgcgc gggggagcct ggtgcgggtg
     1081 gcactcgatg tcacctcctc ggtgccggtt tcgggcctcg aggcacgcat ccagtccacc
     1141 ggcggggcgc gcctcgtcgg caagcgccgc gccgagctcg gtacgctcga tgcgggcgac
     1201 gcgcggcgga cggagttcga catcgagctg ccggccgccg gcaagcgctt cctcctccag
     1261 gtgctcgtga cggccgaggc cgccaccggg ccgatgaacc gcctcgtcgt cctcaacatt
     1321 cttcccgacg gtccggccga tcccggccag gtcgtgacga ctcccaccgg cgaatcgatc
     1381 cgccagtacg cgaacagcgc gcggaggatc gagcgatgaa ctgcctcgaa cgcctcattg
     1441 cccgcagggg ctcggatgcc gccatcggcg gcctgctcat cctgatgtgg gtgctcggcc
     1501 tggccgcctt cgtggtgctc tcggccttcg cctcaccggc cgatgccgct ttcaacgcct
     1561 ccggccgctt cctctacgcg gacaaactct gggacaagga cggctacacg gggcaggagc
     1621 agaacctgcc ggtccgccat gcccgggtgg agatcctcga catcccgacc ctgcaggtga
     1681 tcgggtcggg cgccaccgac aacaacggca actggtcggt cacggtcacg ccgctcctgc
     1741 cgatcgtgcc ggtcaatatc gtggcccgtg tccttaccga cggccgcacc gccggctatg
     1801 aaatccgcgt ggtggacgag ctcgagcgcg gcctcggcga gccggctgtg gtggggaata
     1861 tccacgccgt ccttaccgat ccggtcctga accacaatca gtcacagccg cgggccttcg
     1921 ggacctactt gttgcaggac accgacggca ccggcgtggc ccaggccttc aacatcttcg
     1981 acaacgcggt cgacttcttc gactggatgg cgcagccgag cctcctcggc cggcgcccga
     2041 atgccggcga gtacatcgtg tacgcctggt cgcccatcgg caccaacgag gggtcgaact
     2101 acagcgggca cgccattctc ctctcctcgc ccgggcaggg gaacgacacc gacgcgtgga
     2161 gcgacacggt tatcctccac gagacgggcc actggttcga cgacttcttc tcccgctcgg
     2221 acaaccccgg cggcgcccat tcgctgggtg acaacaacgc caacgtcaag ctggcctatg
     2281 gcgaaggtgt ggccaccttc cattgctcca aggtgcggaa gcttcgcgcc acgacccgca
     2341 ccaacctggt cggacagccg gtcgatcggc atgtgtcgat ctacggcgat ctcaccattc
     2401 cgcccgactc gatgatggcc ggcggcctgt cgttctcgta cgacttcgag accggcgtgg
     2461 ccttccagcc aggccttccg ccgggaggct tcttcctcgg ccagcgggga agcgcgaacg
     2521 agacgaacgt cacctcggcc cagtgggacc tggtggacga cgcctccacg cccgacgaaa
     2581 cgcccggcat cgacgacgac caggtcggcg tatccgatgc cgtctgcttc aacatcgaac
     2641 acaactggct ccccgggctg ccggcctcga acgtcgtgac catcgaggac tactaccagg
     2701 ggtggtatgc ccagaacggc gccaactatc tccgggccgg cgtggacgac atcttcctga
     2761 acctgtcgct catggagttc tatgcggacg gttacgagcc ggatggaacg cccgcccagg
     2821 ccaccgccat cacgccgctg gcttacaccg tgacgccagg cgcgcatctc gtcatcagcg
     2881 agctcgacct cggagcccag gacgcggtgg aagtcttcaa cccgtcggac gccgcggtga
     2941 acatgaccgg ctggacgatc caggtgtacg tgaacgacga caccgcgccg gtggtcagcc
     3001 gcgtctacgt cttcccgccg ttcacgctcg atccgggcga ggcggtggcc gtcctcgaac
     3061 gcggcgatcc cttgaacaac ggcgcgcacc acctgtacgg cggcagcacc gtgccgaatt
     3121 cgtggaatgc ctcgtggaac ttcggcctgg acggcgcctg cgtcttgaac aatgcgtcgc
     3181 agcaggccgt ggacttcgtc acgtggcgcg cggccaacgg cacgccgaac agcacgccgg
     3241 tgccgtccgg cacggccttc acgggggtgc tcgacagccc ggctgccccg ttcaacctgg
     3301 cccgcgacat caacggcacc gacaccgacg ctgccaccga ctggacggcc cgcgagggaa
     3361 cgttggcctc ggccaacggc agcttctccc gtcaccagac cctcttcgac gtgggggacg
     3421 ccgacctgat ccgcttcgac gcggtggccg gccagcggta cggcttcgag gtgcgggcgt
     3481 tctacagcgc cagcgatccc tacgtggagc tcatttcgcc gacgggcacg gtggtcggat
     3541 cgaacgacaa cgtcgatccg gggatcaccg acgcgcgcat cgatctcttt gccggatcca
     3601 ccggcacgta ctacctcaag gtgcgccacg tcgggcccaa taccgactgg gcggcataca
     3661 acgtggccgc cttccagcgg ccgccgagcg acgtgctcct ggcgccgggc ggaatcaccg
     3721 ccacggccgc caacaccaac aacagcggcg acccggtgca cctggagtgg ttgaacggcg
     3781 cggtgtacga caacgtgaag ctgtaccgga acggaacgct tctggccacc ttgcccgggg
     3841 atgtcgacgc ctacgacgat gccgccgacc gcggcgtgta tacgtacgag gtgtcgggcg
     3901 tgcgcggcct caccgagacg gcccgcgtct ccaccaacga gttcgccgga ctggtcggat
     3961 gcttcgtcag tgaggatttc gaggaaggcg cggcacccga ctgggtaagc gaggccaaca
     4021 cctggggcgt gacgccgctg gccgaggggg gcgtgtacgg ctacaccgac agcccggtcg
     4081 gcacctcgcg cggctgccat ggtgtcctcg agaactgctc catgtccacc gccacggtgc
     4141 tccgcgtgcc ggtcatcctg ccgcccggtt cacgtctcct cttcgatcac atctgcatca
     4201 ccgagcactg cgagccggtg ccgtgcgact tcggcgccgt ggagatctcc accgacgagg
     4261 gccagaactg gactgagctg gcccgctacg accaggccag cgatccgggc tgggccgaca
     4321 atgcagccga tccgaccgac tggcggccgg ccgacatctc gctgaacgcc tatacgggac
     4381 agaccgtgct ggtgaggttc cgggtgcaga ccgacccgtt gaccgagctg gacggctggt
     4441 acctcgacaa tgtccgcatc aacgaggggg attgcacgat cgcggccgtc ccgccggccg
     4501 gcggcatccg caccgaactg atggcgccct atcccaaccc gatgacgggg acggcccgct
     4561 tcgccttccg gctggccgaa cctgccccgg acgtgtcgat gtcgatctac gacctgtcgg
     4621 gtcggctcgt ccgtcgcgag gcgctgggcg ccctcgacgc cggcgaccac ctctggcagt
     4681 ggaacggtca gtccgattcg ggcgaaggga cggccaacgg cgtctacttc gcgcggctgg
     4741 aaacggccgg gcagctcttc acccggaaag tcctgaaagt cacccgctag aaatcgctcg
     4801 catcgtcggg ggcctgccgg tcacggctct ggccggcagc tccccccggc gagcgggtct
     4861 tcgcgttatt tgacgccgaa gactttcatg cggaaaaccc ctccgatagt ggcggaattc
     4921 acgaaccctg catatctttc cacaacggct tcgttgcgcg ttcctccccg ccagtcttcc
     4981 cgggtcttct ggatccggtg ggaggatgtc cgtgaacagc gccgaagtcg cacccatcag
     5041 gtcgtggccc gggtggagcc ggggacgtcg cctggccgtc acggtggccc gaggcgctct
     5101 ctggctggcc gctgcccacc cgggccaggc cgatccaacc cgcagtcacc cgctccccga
     5161 tccgctcgag gtagccttcc gcgtggccca cgaggcctcg gggctcaacc gtccggtgat
     5221 cgaagtcatg gccatccagc cggtgcacgt cacactcatg cccgcccagg ccgcgccggc
     5281 gccgggtccc gccgattggc tcgaggtttc cgattcggac gatccgctct tcatttactg
     5341 gcctccgcga ttgccgggcg cggcggttcg cgatgcctgg tatcgacgat ccccggtggt
     5401 gaccccgcat gggcccgggc cactgctcgc cggtcaactt ccagctgacg ggtcggcaag
     5461 cagcaccgcg gacgacgaag cgcggatcga cgacgagttc gaagtgggtg agttcgaagg
     5521 ggtggcaagc gatggtgaac cggtgggact cccgattcgc acggttctgc ccgagccgaa
     5581 cgggggggag gtctggtcgg ccgatagcct ggccacggtg cgctggcagg cggagggggg
     5641 cgtcgatcct cttttcatcc gtctcgagtt ctcggtcgac gatgggcgct cctggcgtct
     5701 cctggccgac tcgctgtaca acgagggctc ctgggattgg gaagtgccct ggctggaagc
     5761 gcggcgggcg cggctgcgcg tgatggcccg cgatgattcg ggcctcgtgg gggtggatgc
     5821 cgccgaccgc tcgttcacga tccgcaagcc ggagtggatc gggatgacgc cgccaccgct
     5881 cctggcgctc gatgccgggt cgcgcctttc ggccggaagc ttccgattgg tggtggagct
     5941 cgccgccgca acgccggtcg aactggtcat tctggcggtg acgggacgat tggtccgccg
     6001 cctgatggac gggccgatgg aggccggcgg ccacgtcgtc gaatgggacg gccgcggtga
     6061 gaacgcgcgg ccggtcgcct cgggcatcta cttcgcccgc ctcatggccc ttggcgaccg
     6121 actggtccgg cgactcgtcc tggttcgggg acgtcggcgc accgggcgcc ggcccggctg
     6181 gtggggcatg acactgccgc gatacactgg ggatcgtcga cccgcctgaa gcccgtcacc
     6241 ccgcccgggc ttcggtccgc gcacgctcgc tcgattggcc gtccagggag tgatcatgcc
     6301 gaagaccgaa gtcatgtcat gtcggccgtc ccggccgacg ctgtcgtcgt ggctcgcatt
     6361 cgcgagcctc ctcatgatga ccaccctcgc gatccatgtt tccagcgcga gcgcccagca
     6421 ggtgcccgac acgacctgga caccatccgg tcacacaccg cactggtcgt cgggcagtgg
     6481 accgcgcgtc gtcatcgacg cggcccacca caatttccac accgtcgacg gccgctaccg
     6541 cccgttcgcc gatctgcttc gccgcgacgg ctatcgcgtc agcggatcga acgagtcgtt
     6601 ccgtgccgcc acgttcagcg gcatcgacat cctcgtcatc gcgaacgcca acagcgcccg
     6661 caacgagagc gactggtcac ttccggtcac ctccgcgttc gagcccgacg aagtcgatgc
     6721 cgtgcgtgcc tgggtgcggg acggaggatc gctcctcctg atcgccgatc acatgccctt
     6781 cccgggggcg gcggccccga tggcggcggc cttcggcatc tcgttccgca atggcttcgc
     6841 cggggctggg catgagctgt cacccatcac gtttcgccgg gggccggtcg gcgtcggttg
     6901 ctttggcggc cagcttgcca cccaccccat tgccaccggg ctccgacccg gggaaggcgt
     6961 ggattcgctg gtcagcttca ccggctcggc ctttcggccg ccggacggcg ccgagccgct
     7021 cctcatcctc ccctcggaca ccatctcgct cgagccggcg caggcgtggc agttcgagga
     7081 acagaccccc cggaccgacg tgggggggtg gtgccagggg gcggtcctcc gctacggacg
     7141 ggggagggtg gctgtcttcg gcgaggccgc catgttctcg gcccagttgc agaccggcag
     7201 cacgcctccc acccccatgg ggatgaacgt cccgagggcc ccgcagaatt tccgcttcct
     7261 tctcaatacc atggcctggc tggcggaatc ggacggcaag gggtcggtaa atacgccgtc
     7321 cacgccaggg accactcgcc agagggccgg gcgctgacca ccgataaccc ctttgaacgg
     7381 tccacccggg gaggccgtct acccccgacg gaggacatga ataccggggt cggaggtcca
     7441 ttggacgcct tgacggacga gcaactcgta gctctgattc aacagggtaa catcaacgcg
     7501 ttcaataact tgtcccagcg ctgggaaacc agcctctacc ggttcatccg gcggatgatg
     7561 ggcaattccg aagacgcccg ggatgtctgc caggaggcct gggccaaggc gtaccagaat
     7621 atcgggacgc tgcgggaggg cgccaagttc agggcctgga tccattacat tgccctgaac
     7681 ctgtgccggg accggttccg gagcctcaaa tccaaggcca tccactccag ttacgaggag
     7741 ggggggctgg aggtcctgcc cgagggaagc gggaagacgg cccagtgggc caccacccgc
     7801 catgtcgagc agacaagtct caagaccctc ctggatgacg cgctgacggc gctgcccaac
     7861 gagcagcgca ccgccatcgt ccttcgggag taccagggtt tcacgtcaga ggagatcgcg
     7921 gagatcaccg gcgtgccggc ggccacggtt cgcagtcgga tattctacgg tttcaaggca
     7981 ctgcgccgaa tccttgggga gaggggcatc tcgccggagc agtcgtcctg aatcgtccga
     8041 aggagtgaat gaattgaaac gacatgaagc ggtcgatccg aaaatcgaca gaatgatcgc
     8101 cgcgctttac ggggagctga acgacgccga gcggctcgaa ttcgagaagc agctcgccaa
     8161 cgacccggcg ctgcgcgccg agtgggagga gatttccggt acccggaacc tcctgaccgg
     8221 ctgggagctc gaggatcggg tgccgagctt cgtcgtcgtc ccggccgtcg ccacccgccc
     8281 gcgcgtggca atgggatggg tggatgtgct gcgtgaacgg ttcggcaact ggttctcggt
     8341 cggcggctgg gtggtggcgg gggctgcggc ggcggtcctc ctcatgctgg ccaaggatat
     8401 ccgcatcgag aagacgccga acgggatcgc gttcaactac ggaggcaagg ccccggtcaa
     8461 ccatgtgctc ccgaccgatc cgtccgggag cgacttcggc ggctcgccgc gcctcgtcaa
     8521 cgacggcgcg gacggtgggc agtacgtgac gaagaacgag ctcgaccagc agtcggcggc
     8581 catccttcgc atgatgtcgg cctacatgga tgactatcgc ggtcggcgcg accaggaact
     8641 cggtgccgcg ctccgttcac tctacggcaa cctcaacgcc cggcaggcct cgcagtacga
     8701 ggatctcctc ggcaagatcg acgccaacca tccgggtgag cgcaccgagc atgaattcct
     8761 gaggcccacc gaactgggca tgagccacgc ggatagcctg gggcccctgc ggctctcgcc
     8821 cgaggacgtc gaggtgggcc ggaatgaata atcgcccccg gtcgttcctg gccggcatcg
     8881 ggctcgtcct ctcgctcctg gttccgggtt tggtccacca ctcgctcgcc gcgccggccg
     8941 cggcccagac atccgaaccg gtccgccaga agcggatgct ccgcgacatc acggtgctcg
     9001 aaaaaatcat cgatgagatg ctcctcgaca gcaagaacct cctcatctac accgcgggag
     9061 gcgtgacgca cggcctctac atcgacgagt tcggcgcgct cttctcgttc gaggcctccc
     9121 tggtcgaccg cagcgacgac tcgccgtggc cggaggggat ggatgcctac accttcgaca
     9181 ccgacgagca cggcaacgtg atcatccgga agaagaagga ttcgaagggg aaggggcccg
     9241 acacgcccgt tccccccacg cctcccaggc ccggcgaaac gccgaaggcg cctcccgcgc
     9301 cgcgcgcatg gatcgaccgc caggccggaa agcaggccaa gctgtacgaa accggcaagg
     9361 ccgagatcat cgacacgctg atcgagtacg gcgattcgat ggatgggctg cgcgacaacc
     9421 agtggctctg cttcgccgcc ttcctcaaga acagcgactt cttcctcgag aaccgcatct
     9481 cgcgccttgt ggtcaaggcc cgaatgagtg acctgcgttc ctacgcgcag ggacgcatcg
     9541 accggaaggc catgatggcc aaggtggtgc aggaagagta ctgaggccgg gcccgcccgc
     9601 caggcaggtg gcccggttac cgggcgatca cgatcggccg caccgcatgg tttgttccgc
     9661 tggtcagccg cgcccagtaa cgacccgacg gaacggggtt accggaatcg tcacggccgt
     9721 cccatcgcac gaccgtcgtt cccgcggtga tctccagttc atccaccaca cccgccacac
     9781 ggcggcccgc cgcatcaaag atggccagcg aggtgcggct cgtacccgct gacgccggga
     9841 tccggaactc gaacgatgcc gcacccgcgg tggggttggg ccaggcgggc gagaggagca
     9901 gggctccggc cgcgtcggac gggatccgga tcgcttcctt ccggatcact tccctcaccg
     9961 agccaccgcg gctgatctcc tcgagcttga tccagagcgt ggcggcatgc aggggggcgg
    10021 tcacgcgcgc ctcgtacgat ccggccgcac cggccgtcac cgggatgagg ctgccggcat
    10081 ccgagtaggc ggcatcccgt gcgggagccg cggaaagtct gaaccccacg gcgccatccg
    10141 ggtcgccgat ccgccaggag acggtgattt cgtcatcctg ccaggtcatg tcgaatgcaa
    10201 ggagcgcgac aggggtcggg cgcgtgccga cggtatacca caccggtgcg tagccgtccg
    10261 aggcccacac ggcgtcgttg ccgctgccgg tcacgccgtc gctgtcgagc cctacccggt
    10321 agatgaccgc atcggagccg gtaacgtact ggtagtagac ctgcgcgtcg tcgccggaaa
    10381 aagtggggcg ccccgtcgat tgctggttgt tggtgatggt gccgctgaca ccgctctgga
    10441 agtcgtaccc gatgacatac acattgccgt cgttcgacgc gtagtcgaag gcgatccggt
    10501 tgtcgctgtt ctgggccagg gccgggttgc ccacgctttc tccctgcggc agcggctgga
    10561 acacgcggaa acattcgccg tccgaaagtc gcagcaggtt gatgtcccag tattcgaaca
    10621 tctggccgcc caggttcgaa cggttcagcg cgtcgtacac gatgtactct ccgtccaggc
    10681 tgaactccat cacatcggcg aagaccacct ggtcggccac gttgccgctg gtggaattct
    10741 gagaggtgag gtcgtagaaa acgttgttct cgccgacgag gtcgaagctg tagatgcggc
    10801 cgtcctcctc cacggtggtg gccgccagtc ggctgccgtc gggcgagaac gccacgctcc
    10861 accagccgcc catgtcggag agcgcctgca cggggccgtc ggcatcgcgt gcgtacacgt
    10921 tgccatccga gccgacgaac gccatgagct gtccgtcgtc cgtgaacgtg ggtcgcccgc
    10981 ctggtcctgt cgcaaggccg gagaggtcga cgacctgccc ctggaacccg ggatcaatgg
    11041 tgacgatgtg gtcattgccg gcgtcgacca cggccacgaa gtcgctgccg acgttgtcgg
    11101 gcgccggtgg cggcggcggc gtcatgccgc catccatgat ctcgacggtg ttcatggcgt
    11161 tggtcacggc cgtcacctcg gccgagccgc ctccgaacag gtcggcggcc gcctggatcg
    11221 tggcgcggcg gaagtccacg aactgcgagt tccgcatcag atactggctg agggcgcggt
    11281 accagatccg ttcgccgcgc gccacgtcga gcgcggtgca ggtgaggtag aaggcgcggt
    11341 tcgggatgcc gctgttgatg tgcaccccgc cgttgtcctg gttgctgttc agattgagga
    11401 actcgctcat cttcgtcggc tgctggccgc cgaaggccac gttggaggcg gccgggttct
    11461 gcatgttgcg cagcgcgtcg cccgccgtgc cgggggtggt cacgtcctcg ccgagcagcc
    11521 agttggcgtt gccgggcgtg gcgaagaact cggtggccgc gccgaacgca tcggcgaagt
    11581 gctcgttcag cgcgcccgac tggaactcgt atacgaggcc ggccgtgttc tcgatgacgc
    11641 cgtggctcat ctcgtgggcc gtcacgtcga gcgcgccggc caggtcggag aactggttgc
    11701 cgtcgccgtt gccgaacacc atgaactggc cgttccagaa ggcgttgttg aaattgttct
    11761 ggaagttcac gaccagccac atcgttccct tgttgccgtc gatgctgaag cgctcgtgcc
    11821 gcgtgcggta gtagtcgtac acctgtgccg cgaagttggc gccgctcacg gcattcctga
    11881 agttggtcca ggtggtcgtg ttgttcgagg tgacatgcgt gaggttatcg cccatgccgt
    11941 gtgcggcgtt gatgagggtg atcgtgccgc tcggcatgtt cggaaaggtg ctggccgcct
    12001 ggttgaacat gtccttccgc gtgtcggtca tgaagaaggt ggagccgatc tggtacaggc
    12061 cgagcgtgcg gttcacgttg gccaggtcgg ggccggagcc ggtcacggcg ccgtcggtct
    12121 ggacgagcga aaggtccttc acgatcgagc cggtccggcc atccacgatc acctcgcggc
    12181 ggcagtcgag gccgccctcc acgatgacgc gataggcagg gaggagcccg cccggcccgt
    12241 cgtaccagac ggggagcgca gcgccggcgg tcgtcgggcg cccctgcgtc acgcgcacat
    12301 ggccgg
//
LOCUS       DASVOD010000288         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_384236, whole genome shotgun sequence.
ACCESSION   DASVOD010000288 DASVOD010000000
VERSION     DASVOD010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384236"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..419
                     /locus_tag="VF720_06425"
     CDS             117..419
                     /locus_tag="VF720_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7879027.1"
                     /translation="MILADVVGTIVSSRKDPAIEGLTLLAVQPLGADLAPSGAVLVAV
                     DSVGAGVGERVLIVSGSSARYTAVTTGKPADATILGIVDLVESNGKRVYDKSRPGR"
     gene            416..1039
                     /locus_tag="VF720_06430"
     CDS             416..1039
                     /locus_tag="VF720_06430"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX7879028.1"
                     /translation="MTPPEALEPGRRDSSEPGRPETPTLALLEIGSMAIGYVAADAMV
                     KAAPVELLAIEPVTPGKLLVLVTGSVAAVEFAMEAGRAVAGEQLLDQLFLPQAHPDVA
                     SVLGLAPTGTIESLGFFECRTVAAGLLSADAAAKASDIRLLTILAARGIGRRTLTLFT
                     GTLHDVEASLAAGNSVLAGRGGALAAVVIPRAHPDFIERLLHPLEVL"
     gene            1036..1908
                     /gene="sppA"
                     /locus_tag="VF720_06435"
     CDS             1036..1908
                     /gene="sppA"
                     /locus_tag="VF720_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX7879029.1"
                     /translation="MTSRTLAWLGCGLVVLFLFGVGVVLMFSIGFMDDGEFPKLGRKV
                     AVVGLTGTIVSSDRLVKTLAEMGEDDAIEAVVLRIDSPGGGIAPSQEIHDALIRLRAR
                     KPVIASMANTAASGGYYVAVACDSIMANPGSLTGSIGVIFSWMTAEELMRKVGVRMEV
                     VKSGPNKDMGSFAREPTDEERRLLEGVVNDAYQQFIDAVTAGRALSRDEVLALADGRV
                     FTGRQALELGLVDRLGGQREAVEWAARMAGIEGEPVVVDRGRSRIPWADWLEESSRTV
                     LPDVAGPRLEYRFR"
     gene            2114..2521
                     /locus_tag="VF720_06440"
     CDS             2114..2521
                     /locus_tag="VF720_06440"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX7879030.1"
                     /translation="MARAATERRKSPRIDARLALQVDRDRKPNGDRPVSSESLNLSAS
                     GLSCQVRSFVPPLTRVGLTLLLPSFGRQGKKTQVINCEGVVVRCADEGMDEGCWEMAC
                     CFTDVRQQDRVLIEEYIGWRLMRDMIDERLSRP"
     gene            2592..>2931
                     /locus_tag="VF720_06445"
     CDS             2592..>2931
                     /locus_tag="VF720_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0164 family protein"
                     /protein_id="HEX7879031.1"
                     /translation="MFRSHHRSTRTRPATFLTLGILTPALLLAVAPTAHAADKFAAEF
                     LKVGVGARALGLGGAFTALADDASAIYWNPAGTAIMGQPDVQLTHAEMFGSIVKHDVI
                     SGVLPIGRDGG"
BASE COUNT          432 a          986 c         1005 g          508 t
ORIGIN      
        1 cgccgatccg gcgcgcgcgg agagcatcct cgtcattgag cggacatccc ccgctcttcc
       61 gggaagggtc cgggcgtcgt tgacgctccg gacccttcct gttagcgtcg cgcgccatga
      121 ttctcgcgga cgtcgtcggc acgatcgtct cgagccggaa ggatccggcc attgagggac
      181 tcacgctgct cgcggtacaa cctctcggtg ccgacctggc cccgtcgggt gccgtcctcg
      241 tcgccgttga ttcggtgggc gccggtgtcg gggagcgggt gctcatcgtt tccggctcaa
      301 gcgcccgcta caccgcggtc acgaccggaa agccggccga tgccacgatc ctcggcatcg
      361 tcgacctggt ggaatcgaac ggaaagcgcg tctacgacaa gtccaggccc gggcggtgac
      421 accgcccgaa gcgctcgagc ccggccgccg cgactcctcc gaaccaggcc gccccgaaac
      481 acccaccctc gcgctcctcg agatcggctc gatggcaatc ggttacgtgg ctgccgatgc
      541 catggtcaag gcggcgccgg tcgagctcct tgccatcgaa ccggtgacgc cgggaaagct
      601 cctcgtcctg gtgacgggct cggtggccgc ggtcgagttc gccatggaag ccgggcgggc
      661 cgtggcgggg gagcagctcc tcgatcagct cttcctcccg caggcgcacc cggacgtggc
      721 ctccgtgctc ggcctcgcgc cgaccggcac cattgagtcg ctcggcttct tcgaatgccg
      781 taccgtggcc gccggcctcc tttccgccga tgcggctgcc aaggccagcg acatccgcct
      841 gctcaccatc ctggcggccc gcggcatcgg caggcgcacg ttgaccctgt tcaccggtac
      901 gctgcacgat gtggaggcct cgctggccgc cggaaattcc gtgctcgcgg ggcggggtgg
      961 ggcgctggcg gcggtggtca ttccgcgagc ccaccccgat ttcatcgaac gactgctcca
     1021 cccgctggag gtgctgtgac atcgcgcacc cttgcctggc tcggttgcgg gctcgtggtt
     1081 ctcttcctgt tcggcgtcgg tgtggtcctg atgttctcga tcggcttcat ggacgacggc
     1141 gaattcccga agctcggccg caaggtggcc gtcgtcgggc tcactggaac catcgtgagc
     1201 agcgaccgcc tcgtcaagac actcgctgaa atgggcgaag acgatgccat cgaggcggtg
     1261 gtcctgcgga tcgactcgcc ggggggcggc attgcgccgt cgcaggaaat ccacgacgcg
     1321 ctgatccgcc tgcgcgcccg gaagccggtg atcgcctcca tggccaacac ggccgcctcg
     1381 ggcggctatt acgtggccgt ggcctgtgat tcgatcatgg ccaatcccgg ctcgctcacc
     1441 ggttcgatcg gtgtcatttt ttcctggatg acggccgagg agctgatgcg gaaggttggg
     1501 gtcaggatgg aagtggtgaa gagcggcccg aacaaggaca tgggctcctt cgcccgcgag
     1561 ccgaccgacg aggaacgccg gctcctcgaa ggggtggtga acgacgccta ccagcagttc
     1621 atcgacgccg tcacggccgg ccgcgccctc tcgcgtgacg aggtgctggc actggcggat
     1681 ggccgggttt tcaccggccg ccaggccctg gagctcggcc tcgtcgaccg gctcgggggg
     1741 cagcgggaag ccgtcgaatg ggccgcgcgc atggcgggaa tcgagggcga accggtagtg
     1801 gtcgaccggg ggcgcagccg gattccgtgg gccgactggc tggaggaatc gtcacgtacg
     1861 gttctccccg atgtggcggg tccgcggctc gagtaccgtt tccgatgagc ggcctcgtcg
     1921 tggcgcgttg aaggtgcctg cggcgctatg ttaagttgtt cgagttaggg cgttgccttt
     1981 cttcaacttg caccgcatcg gcccgattca ccactctaag tgacgatctt ctcccgcggt
     2041 tggaaaccac cgccgggagc gtgggtactt cccgggccgg tttcccgccg gtccggtctg
     2101 gggcggaggg accatggccc gagcggcaac ggaacggcgc aagagtcctc gcatcgatgc
     2161 ccgtctggcc ctgcaggtcg atcgcgacag gaagcccaac ggcgaccgac cggtcagcag
     2221 tgaaagcctg aacctctcgg ccagtggcct gtcgtgccag gtccgaagct tcgtcccgcc
     2281 gctgacacga gtggggctca ccctgcttct cccgtcgttc ggacggcagg gaaagaagac
     2341 gcaggtcatc aactgcgaag gggtggtggt gcggtgcgcg gacgagggga tggacgaggg
     2401 gtgctgggag atggcctgct gcttcaccga cgtccggcag caagatcggg tgctgatcga
     2461 agaatatatc ggctggcgcc tgatgcggga tatgattgac gagcggctgt cccgaccctg
     2521 aggcagcccg ggccccgcgg ggtccaagga cgggggccga tcggccccgg gtcaccctga
     2581 ggagtttctc gatgttccgt tcgcaccacc gctcgacccg gacccgcccc gccacctttc
     2641 tcacccttgg catcctcacg ccggcacttc tcctggccgt tgctcccacc gcccatgcgg
     2701 ccgacaagtt cgccgccgaa ttcctgaagg tcggagtcgg tgcccgtgcg ctcggcctcg
     2761 gcggcgcgtt cacggccctg gctgacgacg ccagcgccat ctactggaac ccggccggaa
     2821 cggccatcat gggccagccc gacgtgcagc tcacgcatgc cgagatgttc ggcagcatcg
     2881 tcaagcacga cgtcatctcg ggcgtcctgc cgatcgggcg ggacggcggg c
//
LOCUS       DASVOD010000289         7144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_906420, whole genome shotgun sequence.
ACCESSION   DASVOD010000289 DASVOD010000000
VERSION     DASVOD010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7144
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906420"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1866)
                     /locus_tag="VF720_06450"
     CDS             complement(<1..1866)
                     /locus_tag="VF720_06450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879032.1"
                     /translation="MNPGPEAAQARRIFGDALDCPPEERAALLDAACGGNESLRRELE
                     SLLRLHPAAGRFFDRAVPPTVHSGSTLPDQFEGVTIGAYRILDEVATGGMGSVYRAER
                     TDQAFQKMVAIKLIRAGLLSPDAVARFKTERQVLADLEHPGIARLLDGGSTADGMPFL
                     VMEFVDGVPLNRHVAERNPGLAERISLFLEICEAVQYAHQNLVVHRDLKPGNILVDVH
                     GHVKLLDFGIAKVLAPSDANAGQTVAHAFTVRYASPEQVRGGRITTATDIYSLGVILY
                     EMLCGVLPYDLDGVSPASVVDTITTREPEKPSRRADPVIARRLAGDLDMIILKALHKE
                     PERRYATVDELAADLKRQRRGEPVLARPDTAAYRISRFVGRNRGLVAGTLGMIVVLIA
                     ALLATLNAYRNAAVSRNNAEFETYIASLAAAEASIVTEKVSEASQHLGKAPPALRGWE
                     WGHLEARLDRSLASFPAHHAGITSLAVAGDGTIAATSVDSTVAILSAEGKVLERHGPF
                     DSAVETARFIDGDRAFVAGLLDGSVIRVERNTGAVRTLFPAGREWAFVSVSPDQRLLA
                     AGFVDGTACVLSLADNRIVREWQAHGVLTVVAYSPDGRYLATAGAEGNVTIHDAKT"
     gene            complement(1850..2428)
                     /locus_tag="VF720_06455"
     CDS             complement(1850..2428)
                     /locus_tag="VF720_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7879033.1"
                     /translation="MSEAQDVTALLQGVTGGSAEATDRLLEIVYDELRTMARRLLGRE
                     RADHTLHPTDLVHEAYLKLIDQTRSTWQNEAHFLAIASQAMRRILVDHARRRGSRKRG
                     QNALKLPLDDALSVAGDDPDTTLLALDLALSRLAGVHPDTARLVELRWFGGLTIDECA
                     RVLEISPRTVTRDWEFAQTWLFREMGGDESGA"
     gene            2602..4110
                     /locus_tag="VF720_06460"
     CDS             2602..4110
                     /locus_tag="VF720_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879034.1"
                     /translation="MARAELTLDPSFGTGGYVHTAFGPQYDAPRDLLILPDGRIVAAG
                     TSLETDSGIHIAMARYSANGVLDTLGFGSAGKVRVRFDYRDYANAIGLQGDGSLVVAG
                     MQMHTNFGSSQIPAVYRFTPNGEVDSGFGVGGTWVSRYDENSTGEHSDLAILADGRIV
                     AAGCSNSSANGGIQGFARKLHATDGTQIGADRIEHNMLTDRGSAAFTPDGGYVGMNTI
                     VLNGHVEFFLGKMTGAGQPDLSFGPGGFRTTGCEIKNGYDYKVMILPSGKILVGATSP
                     KNTFFEKISLFRFSADGVIDPGFGTGGRVDLVYATSNDIFHDMTVDAFGNVIVVGESR
                     SGFLRSLVVRLLPDGVPDESFAPGGGQVLDLDSASSLITQGAIQPDGRIIVAGSDYND
                     FVLARFNAPVTGVADIERPQPIPVALFPNPVRRTAMIEFRLAEPGNVTMALFDGAGRK
                     VRSLLTGALPAGSHEFRWDGRDDSGLETPDGVYFLKLLTPGAGTTRKLIRLQ"
     gene            4140..>7144
                     /locus_tag="VF720_06465"
     CDS             4140..>7144
                     /locus_tag="VF720_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7879035.1"
                     /translation="MRMLLPHRSLPLLFGHIAITSSLALATTPARSEVVDPVTPAGVY
                     TSIAVDGQGRPHIAYYRTGSGDLCYAIKSNGVWTIDVVDTTGSVGTYCSIDLNGSGLP
                     RIAYYDATNGNLKYASKSGSTWSRETVAVSNDVGKACSLALDNAGNPYISCIDETNDD
                     LKLFRKSAGTWFTVTPDADALAGSSTSLVLDSSTIAHIAYKSSTGVRYVTGSGSTWTI
                     QHVMDNANSRTHGDIVSLALDSQGDPHVAFTSWWFVPGFINTHEVTYATRPVNSWTTT
                     VVKAGQNSLSSGSVALVLDPEDDPRIVYRYSGVSGFTIINEFREAELTGGNWDDGLLI
                     DASGLGVGCGAAINSQDELKVSYFDDGLLNYYDSPRYVTVSFPNGGQQFGAFQDVDIA
                     WSTSLSPIANVKLEYGPVGGARQTIVASTPSTGPYPWVVPVAFGSNFRIWVSDASDGS
                     PIDSSNANFSLCEGLSLNGVFGTFSAPNDITAADFNEDGILDMVVCVSGASNGFDVLM
                     GMGSSGVGNGNFNAPVHYAVSAASSARDAAPGDFNRDGILDLAVSVGGGVQMFLGNGT
                     NGVGNGSFTAGSVAAGGSTTRGILVADFDEDGAPDAALVNGASNNISIFRGLGNGTLQ
                     AGVTFNVGTSPSRLLVGDFNEDGIWDLAVTNTSSDNVSILIGQGSGGKGNGAFAAAVN
                     YAAGDGPLGIASGDFEEDGITDLVIADGTTGAVAVLRGNGSGGVGNGTFAAPTSYTFG
                     STARDVGVADMNGDGRADILATAGTGDGMAIFYGSGNIGVGDGGFDFALTVPGDDLAN
                     QMVIGDFEEDGRPDAMATFTNSSTVLLYASGCSPLSGTTALNTTAPDGGEYWVVGTNQ
                     MITWTRGAGVQAVDVEISRDSGANWQKIAVNQTGTSFNWTVTPPQTDDARMRIVDSAV
                     SSRFDAGIDFTIADGPYLLTSPNGGETFRSFASRNIQWVTNTTGDVKLEYRIGSGPKT
                     TITASTPNDGNFSWTVPNPGSSQVRVFVS"
BASE COUNT         1219 a         2355 c         2374 g         1196 t
ORIGIN      
        1 ggtcttcgcg tcgtggatcg tcacgtttcc ctcggctccc gcggtggcca ggtaacgacc
       61 gtcgggcgaa taggccacca ccgtcaggac gccgtgcgcc tgccattccc gcacgatccg
      121 gttgtcggca agagacagga cgcacgccgt gccgtccacg aacccggcgg ccagcaggcg
      181 ctggtcgggc gagaccgaca cgaacgccca ctcgcggccg gcgggaaaga gggtgcggac
      241 ggcgccggtg ttccgctcga cccggatcac gctgccgtcc aggagcccgg ccacgaacgc
      301 gcgatcgccg tcgatgaacc gggcggtttc cacggccgag tcgaacggac cgtggcgctc
      361 gaggactttt ccctccgccg aaaggatcgc caccgtgctg tccaccgacg tggcggcgat
      421 cgtgccgtcg cccgccaccg cgagcgaggt gataccggcg tgatgggccg gaaaggaggc
      481 gaggcttcgg tcgaggcgcg cctcgagatg cccccactcc caaccgcgga gcgccggcgg
      541 ggctttgccg agatgctggc tcgcttccga cactttttcg gtgacgatcg acgcctcggc
      601 cgcggccagg gaggcgatgt aggtttcgaa ttccgcgttg ttgcggctga ccgcggcgtt
      661 ccgataggcg ttgagcgtgg ccagcagcgc cgcgatgagc acgacgatca tgccgagcgt
      721 accggccacc aggccgcgat tccggccgac gaacctcgag atgcgatagg cggccgtgtc
      781 cggccgcgcc agcaccggct cgccccgccg ttggcgcttg aggtcggccg ccagttcatc
      841 gacggtggca tagcgccgct ccggctcctt gtggagggcc ttcaggatga tcatgtcgag
      901 atcgcccgcc aaccgtctgg cgatgacggg gtcggcacgc cggctgggct tctccggctc
      961 gcgcgtcgtg atcgtgtcga ccaccgatgc cggcgacacg ccgtccagat cgtacggaag
     1021 gacgccgcac agcatctcgt agagaatgac gccgaggctg tagatgtcgg tggccgtcgt
     1081 gatccgcccg ccccgcacct gttccgggct ggcgtagcgc accgtgaacg cgtgggccac
     1141 cgtctgaccc gcgttggcgt ccgacggcgc caggaccttg gcgatgccga aatcgagcag
     1201 tttcacgtgc ccgtgcacgt ccaccaggat gttgcccggc ttgaggtccc ggtggaccac
     1261 caggttctgg tgtgcgtact ggacggcttc gcagatctcg aggaagaggg agatccgttc
     1321 ggcgagcccg gggttgcgtt ccgcgacatg acggttgagc ggcacaccgt cgacgaactc
     1381 catgaccagg aacggcatgc cgtcggcggt cgagccccca tcgaggagcc gggcgatccc
     1441 cgggtgctcc aggtccgcga ggacctggcg ctcggtcttg aagcgcgcca cggcgtcggg
     1501 gctcaggagg ccggcgcgaa tcagcttgat ggccaccatc ttctggaacg cctggtcggt
     1561 tcgctcggcg cgatagaccg agcccatgcc gccggtggcc acttcatcga ggatgcggta
     1621 cgcgccgatg gtgacccctt cgaactggtc gggaagcgtc gatccggagt gaacggtcgg
     1681 cggaaccgcc cgatcgaaaa accggccggc cgccgggtgc agacggagca gcgactccag
     1741 ttcccgcctc aggctttcgt tgcccccgca ggcggcgtcg aggagggccg cccgctcctc
     1801 cggcggacaa tcgagggcat ctccgaagat ccggcgggcc tgtgcggcct caggccccgg
     1861 attcatctcc ccccatctcc cggaagagcc aggtctgcgc gaactcccag tcgcgcgtca
     1921 ccgttcgcgg cgaaatttcg aggacacgcg cgcactcgtc gatggtgagg ccgccgaacc
     1981 accgcagttc cacgagccgc gccgtgtcgg ggtgcactcc cgccagtcgg ctcagcgcca
     2041 ggtcgagggc aaggagagtg gtgtcgggat catctcccgc gaccgacagt gcgtcgtcga
     2101 gcggcagctt gagggcattc tgcccgcgct tcctgctgcc gcgccgccgg gcatgatcca
     2161 ccaggatccg gcgcatggcc tggctggcga tggccaggaa gtgggcctcg ttctgccagg
     2221 tggatcgggt ctggtcgatg agcttgaggt aggcctcgtg gacgaggtcg gttggatgga
     2281 gcgtgtggtc ggcgcgctcg cgccccagca ggcgcctggc catggtccga agctcgtcgt
     2341 agacgatctc gagcagacgg tcggtggcct cggccgagcc acccgtaact ccttgcagga
     2401 gtgctgttac gtcctgggcc tcggacatcc gccaatctta tcaataaaag ttggcgtggt
     2461 tcgtcccggt tctacgcgta ccggggaagg gcctttccgg acgacccgga ggcccgaccg
     2521 ggaggccatc caccggaagg attgatcatg tgccgcgccc tccaaatcct gaccccttgc
     2581 atcgctctcc tggtcgcggg aatggcccga gccgagctga cgctcgaccc ttcgttcggg
     2641 acggggggtt acgtccatac ggcattcggt ccccagtatg acgccccgcg ggacctcctg
     2701 atccttccgg acggtcgcat cgtggccgcc gggaccagcc tcgaaacgga cagcggcatc
     2761 catatcgcca tggcgcgata ttcggcgaac ggcgtgctcg acacgctggg gttcggcagc
     2821 gccggcaagg tccgagttcg gttcgactac cgcgactacg cgaacgccat cggtctgcaa
     2881 ggggatggca gcctggtggt tgccggcatg cagatgcata cgaactttgg aagttcgcag
     2941 atcccggcgg tctatcggtt cacgccgaac ggcgaagtgg acagcgggtt cggtgtgggc
     3001 ggcacctggg taagccgcta cgacgagaac tcgacaggag aacactcgga cctggccatc
     3061 ctggcggacg gccggatcgt cgcggccggt tgctccaact cgagtgctaa cggcggcatc
     3121 cagggattcg cccgcaagct ccacgcaacg gacggcaccc agatcggcgc cgacaggatt
     3181 gaacacaaca tgcttaccga tcgcgggtcg gcggcgttca cgcccgatgg cggatacgtc
     3241 gggatgaaca cgatcgtgct caacggccac gtggaattct tcctgggaaa gatgaccggg
     3301 gcgggccagc cggacttgtc gttcggcccc ggcggatttc ggacaaccgg ctgtgaaatc
     3361 aagaacggtt acgactacaa ggtgatgatt ctcccctcag ggaagatcct ggtcggggca
     3421 acgtcgccca agaacacctt cttcgaaaag atcagcctgt ttcgcttcag tgccgacggg
     3481 gtgatcgacc cgggcttcgg caccggcggc cgggtggacc tggtctatgc gaccagcaac
     3541 gacatattcc acgacatgac agtggatgct tttggcaacg tgatcgtggt gggcgaatcg
     3601 cgctccggct tcctgcggtc tctggtcgtc cggctcctgc ccgatggcgt accggatgag
     3661 tccttcgcgc cggggggcgg gcaggttctg gacctggatt ccgccagttc cctgatcacg
     3721 cagggggcaa tccagccgga tgggcggatt attgtggccg gcagcgatta caacgacttt
     3781 gtcctggcgc gattcaacgc cccggttacc ggcgtcgccg acattgagcg gccgcagccc
     3841 atcccggtgg cgctgttccc caatccggtt cgtcgcacgg caatgatcga attccgcctg
     3901 gcggagccgg gcaacgtgac catggccctt tttgacggcg ccggccgcaa ggtgcgcagc
     3961 ctgctcacag gggcgttgcc cgcgggatcc cacgagtttc gatgggatgg acgcgacgac
     4021 tcgggcctgg agacaccgga cggcgtctat ttcctgaagc tgctcacgcc gggcgccggc
     4081 acgacgcgga agctcattcg cctgcaatga tgcgagcctg cccacgataa ggacaccaca
     4141 tgcgcatgtt actgccacac cgatccctcc cactcctctt cggacatatc gccatcacgt
     4201 cctccctggc cctcgccacg accccggccc gctccgaggt cgtcgatccg gtcacgccgg
     4261 cgggcgtgta cacctcgatc gcggtggatg ggcaggggcg gccccacatc gcctattaca
     4321 ggaccggcag cggcgacctc tgttacgcga tcaagtcgaa cggtgtctgg acgatcgatg
     4381 tggtggacac gaccggcagc gtcggaacct actgttccat cgacctgaac ggcagcggac
     4441 ttccccgcat tgcctactac gacgccacca acggcaacct caagtacgcg agcaagtcgg
     4501 gcagcacctg gagccgggag accgtcgcgg tcagcaatga cgtcggcaag gcctgttcgc
     4561 tggccctcga caatgccggc aatccctaca tcagctgcat cgacgaaacc aacgatgacc
     4621 tgaaattatt caggaagtcg gccggcacct ggttcaccgt cacgcccgat gccgacgccc
     4681 tggccgggtc cagtacctcg ctggtcctcg actcctccac gattgcccac atcgcataca
     4741 agtcctcgac cggagtgcga tatgtcacgg ggagcggttc cacgtggacc atccagcatg
     4801 tcatggacaa cgccaacagc cgcacgcacg gcgacatcgt gtccctggcg ctggacagcc
     4861 agggggatcc tcacgtcgcc ttcacgtcct ggtggttcgt gcccggcttc atcaacaccc
     4921 acgaggtgac gtacgcgacg cgtcccgtca attcatggac gacgacggtg gtcaaggctg
     4981 gccagaactc gctgtcaagc gggtcggtgg cgctggttct cgaccccgag gacgatccgc
     5041 gtatcgtcta ccggtattcc ggtgtgagcg gcttcaccat catcaacgag tttcgcgagg
     5101 cggagctgac cggtggaaat tgggacgacg gcctgctcat cgatgccagc ggcctgggcg
     5161 tcggctgcgg ggctgcgatc aattcccagg atgaactcaa ggtttcgtat ttcgatgacg
     5221 ggctcctgaa ctactacgac agcccccgtt acgtgacggt gtccttcccg aacggtggcc
     5281 agcagttcgg cgcgttccag gacgtggaca tcgcctggtc gacctccctc tcaccgatcg
     5341 ccaacgtgaa gctggagtac gggcccgtgg gcggggcacg ccagacgatc gtggcctcca
     5401 cgcccagcac gggtccctat ccctgggtgg ttcccgtggc gttcggcagc aacttccgga
     5461 tctgggtttc cgacgcctcg gatggttctc cgatcgacag cagcaacgcc aacttctccc
     5521 tgtgcgaagg gctttcgctg aacggcgtct tcgggacctt cagcgcgccg aacgacatca
     5581 cggcggccga cttcaacgag gacggcatcc tggacatggt ggtgtgcgtt tccggcgcca
     5641 gcaacggatt cgacgtcctg atggggatgg gctcctccgg cgtcgggaac ggcaacttca
     5701 acgcgccggt gcactacgcc gtgagcgccg cctcgagcgc gcgggacgcg gcaccgggcg
     5761 acttcaaccg ggacggcatc ctagacctgg ccgtttccgt cggcggcggt gtccagatgt
     5821 tcctcgggaa cggcaccaac ggcgtcggga acggatcgtt cacggcgggc agcgtggcgg
     5881 cgggcggcag caccacgcgc ggcatcctgg tggcggactt cgacgaggat ggtgcgccgg
     5941 acgcggcact ggtcaacggc gcgagcaaca acatctcgat cttccgcggc ctcggcaacg
     6001 ggacactgca ggccggtgtg acgttcaacg tcgggaccag cccgtctcgc ctgcttgtcg
     6061 gcgacttcaa cgaggacggc atctgggacc tggcggtaac gaacacctcc agcgacaacg
     6121 tctcgatcct gatcggccag gggagcggcg gaaagggaaa cggtgccttc gcggcggcgg
     6181 tgaactacgc cgccggcgac ggtccgctcg ggatcgcctc gggagatttc gaggaggacg
     6241 gcatcaccga cctggtcatc gccgacggga cgacgggcgc ggtggccgtg ctgcgcggca
     6301 acggctcggg cggcgtgggg aacggcacat tcgcggcgcc gaccagctac accttcggtt
     6361 ccaccgcccg tgacgtcggc gtggccgaca tgaacggcga cggacgggcc gacatcctgg
     6421 ccaccgccgg cacgggcgac ggcatggcga ttttctacgg ctccgggaac atcggcgtgg
     6481 gcgacggcgg tttcgatttc gcgttgacgg ttccgggaga cgacctggcc aatcagatgg
     6541 tgatcggcga tttcgaggag gatggtcggc ccgatgccat ggccaccttc accaacagca
     6601 gcacggtgct gctctacgct tccggatgct cgccactttc cgggacgacg gcgctcaaca
     6661 ccaccgcgcc cgacggtggc gagtactggg tggtcgggac aaaccagatg atcacctgga
     6721 cccggggcgc cggcgtgcag gccgtcgacg tcgagatatc gcgcgacagc ggggccaact
     6781 ggcagaagat tgccgtcaac cagaccggga cctcgttcaa ctggacggtg acgccacccc
     6841 agaccgacga tgcgcgcatg cgtattgtcg actcggcggt gtcgagccgg ttcgacgccg
     6901 gcatcgactt caccattgcc gacggcccct acctcctcac gagccccaat ggcggcgaaa
     6961 cgttccgctc cttcgcgtcc cggaacatcc agtgggtaac gaacacgacc ggcgacgtga
     7021 agctcgagta ccggatcgga agtggcccga agacgacaat caccgcaagc accccgaacg
     7081 acggaaactt ttcgtggacg gttccgaacc ccggctccag ccaggtgcgg gtgttcgtgt
     7141 cgaa
//
LOCUS       DASVOD010000290         6196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_384577, whole genome shotgun sequence.
ACCESSION   DASVOD010000290 DASVOD010000000
VERSION     DASVOD010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6196
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384577"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VF720_06470"
     CDS             complement(<1..768)
                     /locus_tag="VF720_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7879036.1"
                     /translation="MNAETPRGVVDDDAGWDPRDRDDDKDVPARAKRPSKSRIVFQDD
                     DDLNGDAPRFSQELLDQYEESIQAVEEGEIVKGRVLTVDAVNVLVDIGFKSEGVIPLS
                     EFPDPSSIKVGDTIEVYLEKMENQDGLVVLSKQRADFVKVWDRVKEAAEKGEVVEGRL
                     LRKIKGGVVVDLFGVEAFLPGSQIALRPVQNVDALLGQNYRFKIIKLNKRRRNIVVSR
                     RVVLEEERATQKTAIMAELAKDQIREGQVKNITDFGAF"
     gene            complement(993..1691)
                     /gene="cmk"
                     /locus_tag="VF720_06475"
     CDS             complement(993..1691)
                     /gene="cmk"
                     /locus_tag="VF720_06475"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008297019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX7879037.1"
                     /translation="MTNRLRVAIDGPAGVGKTTSARALAGRLGYIYLDTGAMYRALAL
                     KAMRSRVSVDDADGAGDIAAISTVGLLPDGAGGVKVMLDGLDVTASIRTADVSDGASR
                     ISVHRGVRERMVALQQALAASGGVVMEGRDIGTRVIPDAEVKIYLTATAEERARRRHR
                     EMILRGDDASFEEVLELIRERDERDTGREVDPLRPADDAVTIDGTNLSLAEQVDAIEA
                     VVRQVEAGRSAKTT"
     gene            complement(1688..2962)
                     /gene="aroA"
                     /locus_tag="VF720_06480"
     CDS             complement(1688..2962)
                     /gene="aroA"
                     /locus_tag="VF720_06480"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEX7879038.1"
                     /translation="MGRFRPPGDKSISHRSLFLATLAHGESLVSGLNPGVDVATSRSV
                     LTELGAVIRGTANGFAIQGTGGAFRVPDTDLDCGNSGTTMRLLIGMLAGRPGSFRLIG
                     DDSLSRRPMGRVAVPLASMGGSIELSDGDRPPVRVRGSRLTPPAMPEMVRSAQVKSAL
                     ILAGLVARGETTLVESVDSRDHTERLLTLFGIQLKKQGRTITINGPAELRGATVDVPG
                     DPSAAAFFLAAAAMLPGSEVTATGITVNPTRRSAIDVLARMGAVVTTAAGSHRPDHTH
                     DTTGEPVADVTVAAGTLDGTTIEGREAALAVDELPILAVAGCCATGTTIIRDAAELRV
                     KESDRIGGLARGLIELGADVTEQPDGLLIRGGGPGFAFTGGTVDSLGDHRLAMAFRIA
                     ALKATGPVRVIGAECVAISDPAFEETLTGLIA"
     gene            3270..3998
                     /locus_tag="VF720_06485"
     CDS             3270..3998
                     /locus_tag="VF720_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7879039.1"
                     /translation="MIIPAHNEEANVGRVITEIRGFLPTADLVVVDDGSRDNTAQVAR
                     QAGAQVLSLPNNLGYGAAIQTGFRYATRNGYDIGIQMDSDGQHAPESVPALLEPVLRG
                     EIDVALGSRFLGRADYPIPWPKKLGMKLFAGIASAALGTRITDPTTGFQALSSRVMRY
                     FAVDNYPSDYPDADAIMVLGFRGFRIREVPVVMKARMAGTSMHTGYKTVIYVFKMFLS
                     ILVVVLREKLLKPAAPAAATDGGA"
     gene            3995..4450
                     /locus_tag="VF720_06490"
     CDS             3995..4450
                     /locus_tag="VF720_06490"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX7879040.1"
                     /translation="MSMPNGVSEVPVSMADRSIFWMIPLAGFLFVIVLIDMIRKRRLL
                     ETHALLWILTSIVVAVSPLFLPLLNDLAYAVGIFYPPALYLLIAIFFLMANTLRNTLD
                     ISRLTEQNRRLTQEVAILRNEIERPSSVESGGTTTAGRAASLPGPPPGP"
     gene            4447..>6196
                     /locus_tag="VF720_06495"
     CDS             4447..>6196
                     /locus_tag="VF720_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879041.1"
                     /translation="MRPSLPGRSRSSVPADTRPPAPLSGTFVFLALVLLLLHAWILRG
                     FLVDDAFISWRYARHLAEGLGPVFNPGERVEGFSNPLYTMLMGVVAWLVGIFGGGRDA
                     VIAHLPGLGRAVGVAAALGTIVAMARWRDVSRAPGTLLAVLLTATSTSLALWSVGGLE
                     TTLYGFFLAAAFGATLAQPRTTMGRLGTGLLLAAVALSRPEGIVPAGALFLVRWLDPA
                     TRDERRGHLEVGLAFALPVLAWFLFRVTWFGDWLPNTYYAKKLGLAEAWRKGSWYLVS
                     FVHHNGGRWLYAPAVLAFFDRPRRRLAWTAGFVLAAYIPYILFVGGDWMDQHRFLAPV
                     VPFIYLLTGLGWGVLIETGRRLLVLWPGEGRLVDVAVELGVLVAWCLLLRPTYDLTRA
                     QVAGGGYVNARPYYETVGREISVLIGDGGRPGWRGLVWPHEGAPPVGSAATHDIGAIG
                     WYGGVGIVDLLGLVDRDLAKGRVDAEELIARERPDLVVLHYDNRSSDDPSRPRWRELE
                     AHGLDTLYVPVAIHGYDGLPWLRVRSDREAEVRRRWEEVRTRALASSIPSAAERNDGG
                     RRRSDRAPRTRGSERLR"
BASE COUNT          884 a         2206 c         1991 g         1115 t
ORIGIN      
        1 gaaggcgccg aagtcggtga tgttcttgac ctgtccctcg cggatctggt ccttggcgag
       61 ctcggccatg atcgccgtct tctgcgtggc gcgctcttcc tcgagcacga ccctcctgct
      121 gaccacgatg ttccggcgac gcttgttcag cttgatgatc ttgaagcggt agttctgccc
      181 cagcagcgca tcgacgttct gcaccgggcg cagcgcgatc tggctgcccg ggaggaacgc
      241 ctcgacgccg aacaggtcga cgacgacccc acccttgatc ttgcggagca ggcgtccttc
      301 caccacttcg cccttctcgg ccgcttcctt gacgcggtcc cagaccttga cgaagtcggc
      361 gcgctgcttg gagaggacga cgaggccgtc ctggttctcc atcttctcga ggtagacctc
      421 gatcgtgtcg ccgaccttga tcgacgacgg atcggggaac tcggagagcg gaatcacgcc
      481 ttccgacttg aatccgatat cgacgagcac gttgacggca tcgaccgtca ggacgcgacc
      541 cttgacgatc tcgccctcct cgacggcctg gatcgactcc tcgtactggt cgagcagttc
      601 ctgggaaaag cggggcgcat cgccgttcag gtcatcgtcg tcctgaaaaa cgatccggct
      661 cttgctgggg cgcttggcac gcgccgggac atccttgtcg tcgtcccgat cgcgagggtc
      721 ccagccggcg tcgtcatcca cgacgccccg tggggtttcc gcattcattc gtgcaacctc
      781 caacttcggt actgccgccg ttgtgggcgt gcggccggcg tgatgcccgg gccgccgttc
      841 ccgctacggg cgaacggtgg tcgcgaaaca cgcccgggaa cggggcggcg gtcggtcgac
      901 cggtttcgcg tggcgggttt caccgccttc tggattctgt tccctggaaa aaaactatct
      961 ggattgcgct cggcctttcg gccgctcgcg actcaagtcg tcttcgcgga acgtcccgct
     1021 tccacctgcc ggacgacggc ctcgatggcg tcgacctgtt cggcgagcga caggttcgtc
     1081 ccgtcgatcg tcaccgcgtc gtcggccggc cggagaggat cgacctcccg cccggtatcg
     1141 cgctcgtccc gctcgcggat cagttccagc acctcctcga aggaggcgtc gtccccgcgc
     1201 aggatcattt cgcggtgccg ccgccgcgcc cgttcctcgg ccgtggcggt cagatatatc
     1261 ttcacctcgg cgtccgggat gacccgggtg ccgatgtctc gtccttccat caccacgcca
     1321 ccggaggccg ccaaggcctg ctgcagcgcc accattcgtt cccgcacgcc ccggtgcacc
     1381 gagatgcgcg acgcaccgtc ggacacatcg gccgtccgga tgctggccgt gacatccagc
     1441 ccgtccagca tcaccttcac gcccccagcc ccatcgggca ggagccccac cgtgctgatc
     1501 gccgcgatat cgcccgcacc gtccgcatcg tcgaccgaaa cccggctccg catggccttg
     1561 agtgccagcg cccggtacat cgcccccgta tcgaggtaga tgtaaccgag tcgtcccgcc
     1621 agggcccggg cggaagtcgt cttgcccact cccgcgggac cgtcgattgc cacacgaagg
     1681 cgattcgtca cgcaatcaac cccgtcaacg tttcctcgaa tgccggatcc gaaatcgcca
     1741 cgcactccgc cccgatcacc ctgaccggcc cggtggcctt gagcgccgcg atccggaacg
     1801 ccatcgccag ccggtgatcc ccgagggaat cgaccgtgcc gccggtgaag gcgaaccccg
     1861 gaccacctcc ccggatgagg agaccgtcgg gctgttcggt cacgtccgcg cccagttcga
     1921 tgaggccgcg tgcgaggcca ccgatccggt cactttcctt gacgcgcagt tccgccgcgt
     1981 cgcggatgat cgtggttccc gtcgcgcaac agcccgcaac ggccaggatc ggcagttcat
     2041 cgacggccag ggccgcttcg cgcccctcga tcgtcgttcc atccagcgtt ccggcggcca
     2101 ccgtgacatc ggccaccggc tcaccggtcg tatcgtgcgt atggtccggc cggtgacttc
     2161 ccgccgccgt cgtcaccacc gcgcccatgc gggccaggac atcgatggca ctccggcgcg
     2221 tcgggttgac cgtgattccg gtggcggtga cttccgaacc cggcagcatg gccgccgcgg
     2281 ccaggaagaa cgccgccgcc gagggatccc ccggcacatc gaccgtggcg ccgcgaagct
     2341 ccgccggccc gttgatcgtg atcgtgcgcc cctgcttctt cagctgtatg ccgaacaggg
     2401 tcaggagccg ctcggtgtga tcccgggagt cgacgctctc cacgagcgtc gtttcgccgc
     2461 gggccacaag gcccgccagg atcagcgccg acttcacctg cgcgctgcgt accatctccg
     2521 gcattgccgg cggggtgagc cgggagccgc gaacccggac cggcggccga tcgccgtcgg
     2581 agagttcgat gcttccaccc atactcgcaa gcggcaccgc cacgcgtccc atcgggcggc
     2641 gtgacaacga gtcgtccccg atcaatcgga acgaccccgg ccggccggcc agcatcccga
     2701 tcaggaggcg catggtcgtg ccggaattgc cgcaatcgag atcggtgtcg gggacgcgaa
     2761 acgcgccgcc ggttccctgg atggcgaaac cattggccgt gcccctgatg accgcgccca
     2821 gctccgtcag gacggaacga ctggtcgcga catccacgcc cggattcaaa ccggacacga
     2881 gcgactcgcc gtgggccagt gttgccagga agagggaacg atgactgatc gacttgtcgc
     2941 cgggcggccg aaaccgaccc atcaagcggg gggcctcgtg cacgacgtgt tcgactcccg
     3001 atgccaactt ccagaccgac tcgaagccgg aggagattaa ccgacccggg aggccccgtc
     3061 caccggattc tgcggcaaat ctaaaggcga cataggcttc tgccgatgac tcaaggccgg
     3121 cgggagaggc ctgccgggca tgggcgccgg agcactgcct ggggggtggc cagagcgtcg
     3181 ctccgggggg tttcttcacg cccaccctcg cgcgttcctt gagcgcgatc ctcctttcca
     3241 ttacagtggc gcaacttatg cggattctcg tgatcatccc ggcgcacaat gaagaggcca
     3301 acgtgggccg cgtcatcacc gagatccggg gtttcctccc caccgccgac ctcgtggtgg
     3361 tggacgacgg ctcccgggac aacaccgccc aggtggcccg ccaggccggc gcccaggtcc
     3421 tttctctccc caacaacctg ggttacggtg ccgccatcca gaccggcttc cgctacgcca
     3481 cgcggaacgg ctatgacatc ggcatccaga tggactcgga tggccaacac gcccccgaat
     3541 ccgtgcccgc cctgctcgaa ccggtcctcc ggggcgaaat cgacgtggcg cttgggtcac
     3601 gctttctggg ccgcgccgac tatccgatcc cctggcccaa gaaactgggc atgaagctct
     3661 tcgccggcat cgcctcggcg gcgctcggta cccgcatcac cgatccgacg accggcttcc
     3721 aggcgctcag cagccgggtg atgcgctact tcgccgtgga caactatcca tcggactacc
     3781 ctgacgccga cgccatcatg gtgctcggct tccgcgggtt ccgcatccgc gaagtgcctg
     3841 tggtgatgaa ggcccgcatg gccggcacgt cgatgcacac gggctacaag acggtgatct
     3901 acgtcttcaa gatgttcctg tcgatcctgg tggtcgtcct gcgcgagaag ctgctcaagc
     3961 cggccgcccc cgcggcggcc acggacggag gggcatgagc atgccgaacg gcgtttccga
     4021 agtgccggtg tcgatggccg atcgctcgat tttctggatg atcccgctgg ccgggttcct
     4081 cttcgtgatc gtattgatcg acatgatccg caagcggcgg ctcctggaaa ctcacgcgct
     4141 cctctggatc ctgacctcga tcgtcgtggc ggtgagcccc ctcttcctgc cgctcctcaa
     4201 tgacctggcg tacgccgtcg ggatcttcta cccgccggcg ctctatctcc tgatcgccat
     4261 cttctttctc atggccaaca ccctgcggaa cacgctcgac atctcgcgcc tgaccgagca
     4321 gaatcggcgc ctgacgcagg aggtggccat ccttcgaaac gaaatcgaga gaccgagttc
     4381 ggtcgaatcc ggcggcacta caaccgccgg ccgcgccgct tcccttccgg gaccgccgcc
     4441 cggcccgtga ggccgtcgct gcccggccgt tcgcgctcgt ccgtacctgc cgatacgcgc
     4501 ccgcccgcgc ccctctccgg cacgttcgtc ttcctcgcgc tcgtcctgct cctgctccat
     4561 gcctggatcc tgcgcggttt cctggtggat gacgccttca tcagctggcg ctacgcgcga
     4621 cacctcgccg aggggctggg gcccgtgttc aatccgggcg agcgcgtcga agggttctcc
     4681 aaccccctct acacgatgct catgggcgtg gtggcctggc tggtcgggat tttcggcggt
     4741 ggccgcgatg cggtaatcgc ccatctgccc ggcctggggc gcgccgtcgg cgtggcggcc
     4801 gcgctcggca cgatcgtggc gatggcgcgc tggcgtgatg tttcccgggc gccgggaact
     4861 ctcctcgccg tgctgctcac tgccacgagc accagcctgg cgctctggtc ggtggggggg
     4921 ctcgaaacga cgttgtacgg atttttcctg gcggcagcgt tcggcgccac gctggcccag
     4981 ccgcggacca cgatggggcg gctgggcacg gggctgctcc ttgccgcggt ggccctgagc
     5041 cgacccgagg ggatcgtgcc cgcaggtgcg ctgttcctcg ttcgctggct cgatccggcc
     5101 acgcgcgacg agcggcgcgg tcatctggaa gtgggtcttg ccttcgcgct cccggtgctg
     5161 gcctggttcc tcttccgggt gacctggttc ggcgactggc tgccgaatac ctattacgcc
     5221 aagaagctcg ggcttgccga ggcgtggcgc aaggggtcgt ggtacctcgt gtcgttcgtg
     5281 catcacaacg gcggacgctg gctctacgct ccggcggtgc tggccttctt cgaccgcccg
     5341 cgccgacggc tggcgtggac cgccggtttc gtcctcgcgg cctacatccc ctacatcctt
     5401 ttcgtgggcg gcgactggat ggaccagcat cgctttctcg cgccggtcgt gccgttcatc
     5461 tatctcctga ccggcctcgg ctggggggtg ctgatcgaga ccggccgtcg tttgctcgtc
     5521 ctctggcccg gcgaggggcg gctggtggac gtggccgtcg agctgggcgt gctggtcgcc
     5581 tggtgcctgc tgctccgccc gacttacgac ttgacgcgtg cgcaggtggc cggcggcggt
     5641 tacgtgaacg cgcgccccta ctatgaaacc gtcggccggg agatttcagt gttgatcggt
     5701 gacggcgggc ggcccggctg gcgcggactc gtctggcccc acgaaggcgc cccccccgtc
     5761 ggctcggccg cgacgcacga catcggcgcc atcggctggt acggcggcgt cgggatcgtc
     5821 gacctcctcg gcctcgtcga tcgcgacctg gccaaggggc gcgtggatgc cgaagagctg
     5881 attgcccgtg agcggcccga tttggtggtt ctgcactacg acaatcgctc gagcgatgac
     5941 ccctcccgcc cccgctggcg cgagcttgag gcgcacggtc tcgacacgct gtacgtgccg
     6001 gtggcgatcc acggttacga tggcttgccc tggctccgtg tgcgttccga tcgcgaggcc
     6061 gaggtgcggc gccgctggga ggaggtgcgc acgcgcgcgc tggcttcgtc gattccgtcg
     6121 gcggcggagc gcaacgatgg cggtcggcgc cggtcggacc gcgccccgcg tacgcgtggg
     6181 tcggagcgcc tccgcg
//
LOCUS       DASVOD010000291         7273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_854483, whole genome shotgun sequence.
ACCESSION   DASVOD010000291 DASVOD010000000
VERSION     DASVOD010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854483"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VF720_06500"
     CDS             <1..335
                     /locus_tag="VF720_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7879042.1"
                     /translation="AVPDEPSPVTGPPADASRLLLAFPNPFGASDAIKFRVDEGAPVR
                     EVKLRLYDLQGRLVKAFILEDLMPPGEYTVKWDTHDNRGEPLRAGIYFLRLNVDRKSE
                     SVKIMIRQ"
     gene            complement(472..1317)
                     /locus_tag="VF720_06505"
     CDS             complement(472..1317)
                     /locus_tag="VF720_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879043.1"
                     /translation="MPLRLVKSGQTIETAGDWRRSERSTGASRSAHYLLGHNELTEAF
                     FGKGYPEVPWEIVEAGTSHPDLEGLSLEWGIPSHRVAIGPFGAEHDQTDVVLVGSCRD
                     RRVAVSVDAVADESFGDGLADFVRRWTLPPERPRTDAVAAMPPEGRRPAPAPERPDRR
                     PEELAELIRALVPDAPPSIKAVLRLRLLLAVASALVFARRQHAHVALLAILEFDRDDW
                     PGRGTREHRYELNDLAVALTGPGAALSPGDCLGPIRVPGNELIPGDIPVYLAMGHRNA
                     GRHFH"
     gene            complement(1384..3396)
                     /locus_tag="VF720_06510"
     CDS             complement(1384..3396)
                     /locus_tag="VF720_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7879044.1"
                     /translation="MIQLKSLSYTIGPRTLFDGLDWVLAPGDRWALVGPNGAGKTTLI
                     KVILGQLDPASGERVMARGTRLGYLPQEAAERFDGTVLDRAMEAYRELLDARIELDAI
                     QDRLAHDATGHADPADATGPAAGAAARHAADLDKAGELQTFLERHDEHTLEPRARRIL
                     GGLGFSPTDQDRPLAEFSGGWRMRAALAALLLAEPTFLFLDEPTNHLDLPAMEWLEDY
                     LDDFDGGLVIISHDRVFLDRVADGVMELEHASLTPYPMGFSQYLVAREETRERMAAEN
                     EQLDKKIAQLSRFVERFGAKNTKATQAQSKRKMIERLASERKIIPRRSKSISFSFPPP
                     PHAGKLLLALSDVRFAYPGGPDIIANATLEIARGDKVAIVGANGAGKTTLLRLLAGQL
                     APAAGDVRVFELTRSGYFAQHAAETLDPAVTALGALEEKATDAWRPKLRSLLGNFLLT
                     GDDAFKPCRVLSGGERQRVALARLLLESTNLVLLDEPTHHLDLAGKDVLEEALSQYPG
                     GVVVVTHDRSLMARVATRVIEVNDGRAIVYPGGYDDYESARVARNARGTGAPAPVPGP
                     GPTPSRGSTPLARANSSPVATAPQAATAQPQPSKNTQKKRAEAMQAVERRIEASEAAL
                     KRVEEGLADPATYAAGAETRAKELVGEQRRLRAELDALWKSLEEFA"
     gene            complement(3475..3978)
                     /locus_tag="VF720_06515"
     CDS             complement(3475..3978)
                     /locus_tag="VF720_06515"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX7879045.1"
                     /translation="MFDLIRQMMGKGHTGSENRPELGAANPAPGRSGAPPDEVAVAAC
                     ALLLEIAHADGEFAPEERRHIESAMARHFNLDSDAIATLMAAAEAERKDSVDHFQFTR
                     QIAAGFDLGQKTLLAEIMWGVILADGRIANHEEYLVRKVGNLLNLEPGYLSQARRAAG
                     EKEKGQG"
     gene            complement(4063..5076)
                     /gene="mtnA"
                     /locus_tag="VF720_06520"
     CDS             complement(4063..5076)
                     /gene="mtnA"
                     /locus_tag="VF720_06520"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEX7879046.1"
                     /translation="MLETVRWHDGQVRWIDQRLLPGRLHVITSASLEELAQAIETLAV
                     RGAPAIGIAAAFGIALAAQQAATNGTPMTGALAEADVRLRRTRPTAVNLFWALDRMTR
                     VAGAHGSGMAANDLAARLLKEAELILAEDLETSRRIGTFGLALLKDGARVLTHCNAGG
                     LATGGLGTALAPVYAAQAAGRKVEVFADETRPLLQGARLTAWEMANADIPVTILCDGA
                     APGLLHSGRIDIVIVGADRVARNGDTANKVGTFGAALAAKSAGVPFYVAAPLSTFDPK
                     IAAGRDIPIEERGGDEVTTTTAAGAPDGVRVYNPAFDVTPAALIAGWITERGIIHPPF
                     EGD"
     gene            complement(5159..5728)
                     /locus_tag="VF720_06525"
     CDS             complement(5159..5728)
                     /locus_tag="VF720_06525"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019024837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HEX7879047.1"
                     /translation="MEEPVVLASASPRRTEILSRAGIAHVVAPVDADESLVAGDSPDI
                     QALTIARRKAEAAAPRWPGRWVLAADTIVDVGGVPLGKPADDSDARRMLGALSGRPHA
                     VHTGLALVTPSGTVHTAVESTRVHVRTLTPEVIDRYVASGEPRDKAGAYAIQGLGSLL
                     IDRIDGAYDNVVGLPLARLLTLFAEARHR"
     gene            complement(5954..6391)
                     /gene="dtd"
                     /locus_tag="VF720_06530"
                     /pseudo
     CDS             complement(5954..6391)
                     /gene="dtd"
                     /locus_tag="VF720_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
     gene            complement(6402..>7273)
                     /locus_tag="VF720_06535"
     CDS             complement(6402..>7273)
                     /locus_tag="VF720_06535"
                     /inference="COORDINATES: protein motif:HMM:NF040246.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX7879048.1"
                     /translation="KYALKNGDSVQIITSPNAHPSDEWLSIVKSSRARQKVRRWLTEQ
                     RMEHSIELGRDMLEREGRRFHKKLPSDRELIDVAQSFGFPDVERLLRSVGEGHTGAHN
                     VLTRCFPEIIQKREPAPTALSKIRDFAVGGGKGISIQDVGNLMIVFARCCQPVPGDQV
                     IGVVTRGRGLSVHRIDCPNGFEEKVGKDRRMELNWNVSEEKAFVVKLIIHGDDRQSFL
                     ADVANAVSQTQTNIKNADIKSADGEALGTFLVEVKNLNHLQRVIKAIRNIRGVTSVER
                     HQVLGEDEGETRH"
BASE COUNT         1077 a         2377 c         2628 g         1191 t
ORIGIN      
        1 cggcggtgcc ggacgagccg tcgcccgtga cgggcccgcc ggccgacgcg tcaaggctgc
       61 tcctggcctt cccgaacccg ttcggcgcca gcgatgccat caagttccgg gtggacgagg
      121 gcgccccggt ccgcgaggtg aagctgcgac tgtatgacct tcagggtcgt ctcgtgaagg
      181 cgttcatcct cgaagatctc atgccgcccg gcgagtacac ggtgaagtgg gacacgcacg
      241 acaaccgcgg cgagccgctc cgggccggga tctacttcct gcgcctgaac gtggacagga
      301 agtccgagtc ggtgaagatc atgatccggc agtagggttc acgcgcagcg cttcggcctg
      361 agtttgattc aggtctgttg ggcgtggtga cctgtgttca atacagattg cgacggcggc
      421 cggtcctttt cgaaggatcg gccgccgagt ttttcaggca ggcgcgaacg tctagtgaaa
      481 gtgacgcccc gcatttcgat gccccatggc caaatagacg gggatgtcgc ccgggatgag
      541 ctcattgccc ggcacccgga tggggccgag acaatcgccc ggcgagaggg cggcgccggg
      601 gccggtgagc gcgaccgcga ggtcgttcaa ctcgtagcga tgctcgcgcg tgccgcggcc
      661 gggccagtcg tcccgatcga attccaggat ggccaggagc gccacgtggg cgtgttgtcg
      721 gcgggcgaag accagggcgc tggccacggc aaggaggaga cgcagccgca gcacggcctt
      781 gatcgacggc ggggcgtcgg gaacgagcgc gcggatcagc tcggcgagtt cctcgggccg
      841 ccggtcagga cgctcgggcg cgggcgcggg tcggcgccct tccggcggca tggcggcgac
      901 ggcgtctgtc cggggacgct cgggcggcaa cgtccagcgg cgcacgaaat cggccagccc
      961 gtcgccgaac gattcgtcgg cgacggcatc gaccgagacg gccacgcgtc ggtcgcggca
     1021 ggagcccacc aggacgacgt cggtctggtc gtgctcggcg ccgaaggggc cgatggccac
     1081 gcggtgggaa ggaattcccc actcgaggga gagcccctcg aggtcggggt gcgaggtgcc
     1141 ggcctcgacg atttcccagg ggacctcggg ataacccttc ccgaaaaagg cctcggtgag
     1201 ctcgttgtga ccgagcaggt agtgcgccga ccgggaggcg cctgtggagc gttcgctccg
     1261 gcgccagtca ccggcggttt caatggtttg accgctcttc acgagtcgca gcggcatgtg
     1321 tcctccgatg cgggggtagg tcgcccgagc ctagcacacg gcgcggggcg tcaagcgaat
     1381 tccctacgcg aattcctcga gcgatttcca gagggcatcg agctcggccc gaaggcggcg
     1441 ctgttccccc accaactcct tcgcccgggt ctcggcgccc gcggcatagg tggccggatc
     1501 ggccagtccc tcctccacgc gcttgagcgc cgcctcggat gcctcgatac gcctctcgac
     1561 ggcctgcatg gcctcggcgc gcttcttctg ggtgttcttg gagggttggg gttgggcggt
     1621 ggctgcctgg ggcgccgtgg cgacgggcga tgagttggcg cgtgccaacg gcgtcgagcc
     1681 ccgagacggc gtcgggcccg ggcccggaac cggcgccgga gcgcccgtcc ccctcgcgtt
     1741 tcgcgccacc cgtgcgctct cgtaatcgtc gtagccaccc gggtagacga tcgcccggcc
     1801 gtcgttcact tcaatgacgc gtgtggccac gcgggccatc agcgagcggt cgtgcgtgac
     1861 gacaaccacc ccgcccggat actgcgagag ggcctcctcg agcacgtcct tgccggccag
     1921 gtcgagatga tgcgtgggct cgtcgagcag gacgaggttg gtcgactcga ggaggaggcg
     1981 cgccagcgcc acccgctggc gctcgccgcc cgagaggacg cggcacggct tgaaggcgtc
     2041 gtcgccggtc aggaggaagt tgccgagcag gctgcgcaac ttcggccgcc aggcatcggt
     2101 ggctttctcc tcgagggccc cgagggccgt tacggccgga tcgagcgttt cggcggcgtg
     2161 ctgcgcgaag tagccggagc gggtgagctc gaagacgcgc acgtcgccgg cggcgggggc
     2221 cagctggccg gccaggaggc gcaggagcgt ggtcttcccc gcaccattgg cgccgacgat
     2281 ggccaccttg tccccccggg cgatttcgag cgtggcattc gcgatgatgt ccggcccgcc
     2341 ggggtaggcg aaccgaacgt cactcagggc caggagaagc tttccggcgt ggggcggcgg
     2401 cgggaaactg aatgaaatgg acttcgagcg gcgcgggatg atcttccgct ccgacgccaa
     2461 ccgctcgatc atcttccgct tgctctgcgc ctgggtggcc ttggtgttct tggcgccgaa
     2521 ccgctccacg aaccggctga gctgggcgat cttcttgtcg agctgctcgt tctccgcggc
     2581 catccgctcg cgggtttcct cgcgggccac gagatactgg ctgaacccca tcggatacgg
     2641 agtgagcgag gcatgctcga gctccatcac gccatcggcc acgcgatcga ggaaaacgcg
     2701 atcgtgggag atgatgacga ggccgccgtc gaagtcgtcc aggtagtcct cgagccactc
     2761 catggccgga aggtcgaggt ggttggtcgg ctcgtcgagg aagaggaagg tcggttcggc
     2821 aaggaggagc gccgccaggg cggcgcgcat ccgccagccg ccggaaaatt cggcgagtgg
     2881 gcgatcctgg tccgtcggcg agaagccgag gccgccaagg atgcggcggg cgcggggttc
     2941 cagggtgtgc tcgtcgtggc gttcgaggaa tgtctggagc tcgccggctt tgtcgaggtc
     3001 ggcggcgtgg cgggcggcag cgccggcggc aggcccggtc gcgtcggccg ggtcggcatg
     3061 tccggtcgcg tcgtgagcca gtcgatcctg tatcgcatcg agttcgatac gggcgtcgag
     3121 gagttcgcgg tacgcctcca tcgcccgatc gaggacggtg ccgtcgaacc gctcggccgc
     3181 ctcctggggg aggtagccga gtcgcgtgcc gcgcgccatc acccgctcgc ctgaggcggg
     3241 atcgagctga ccgaggatca ccttgatgag cgtggtcttg ccggcgccgt tcgggccgac
     3301 gagggcccag cgatcgccgg gagcgagcac ccagtcgagg ccgtcgaaga gggtgcgcgg
     3361 gccgatggtg tagctgaggg attttagttg gatcatcggg tggatcgggt gcgatcggtc
     3421 gacatcagcg aatgcccggc gcaaggccgc ttgccgccgg tgtcgattgc ggtgtcagcc
     3481 ctgtcctttc tccttctctc ccgccgcccg ccgcgcctgg ctcagatatc ccggctcgag
     3541 gttgagcagg tttcccacct tccgaacgag atattcctcg tggttggcga tccggccatc
     3601 ggcgaggatg accccccaca tgatctcggc gagcagcgtc ttctgtccga gatcgaagcc
     3661 ggcggcgatc tggcgggtga actggaagtg gtcgaccgag tccttgcgtt cggcctcggc
     3721 ggcggccatg agagtggcga tggcgtcgga atccagattg aagtggcgcg ccatcgccga
     3781 ttcgatgtgc cggcgttcct cgggagcgaa ctcgccgtcg gcatgggcga tctcgaggag
     3841 gagggcgcag gcggcgacgg ctacttcgtc gggtggggcg ccggaccgcc cgggggccgg
     3901 attcgcggcg ccgagttcgg gccggttctc gctcccggtg tgccccttcc ccatcatctg
     3961 tcgaatcagg tcgaacatca gttactcctc aggtcggctg tcaggtacct gacaggtccg
     4021 atatcaggcg ggtgacacgc ccgatatcag gtaagccctg gatcagtcac cctcgaacgg
     4081 cgggtggatg atgccacgct cggtgatcca ccccgcgatc agtgcggcgg gagtcacgtc
     4141 gaaggccgga ttgtagacgc ggacgccgtc gggcgcgccg gcggcggtgg tggtcgtgac
     4201 ctcgtcgcca ccgcgctctt cgatcgggat atcacgaccg gcggcgatct tcgggtcgaa
     4261 cgtggagagc ggcgcggcca cgtagaacgg cacgccggcc gacttcgcgg cgagcgcggc
     4321 gccgaaggtg cccaccttgt tggccgtgtc gccattgcgg gccacgcggt cggcgccgac
     4381 gatgacgatg tcgatccggc cggaatggag caggccgggc gcggcgccgt cgcacaggat
     4441 cgtcacgggg atgtcggcgt tggccatctc ccaggcggtc agccgggccc cctggaggag
     4501 cgggcgggtt tcatcggcga acacctcgac ctttcggccg gccgcctgcg cggcgtacac
     4561 gggtgcgagc gcggtgccga ggccgccggt cgcgagtccg ccggcattac agtgagtgag
     4621 gacgcgggcg ccgtccttca ggagcgccag gccgaacgtg ccgatccggc gcgaggtttc
     4681 caggtcctcg gccaggatga gctccgcttc cttgaggagt cgcgccgcca ggtcgttcgc
     4741 cgccatcccg ctgccgtgcg cgcccgccac gcgcgtcatc cggtcgagcg cccagaagag
     4801 gttgaccgcc gtgggacgcg tgcgccggag gcgcacatcc gcctcggcga gcgcgccggt
     4861 catcggtgtt ccgttggtcg cggcctgctg ggccgccagc gcgatgccga aggccgcggc
     4921 aatgccgatg gccggggcac cgcggaccgc cagcgtctcg atcgcctggg ccagctcctc
     4981 gagactggcc gacgtgatga cgtgcagccg gcccggcagg agacgctggt cgatccagcg
     5041 gacctgtcca tcgtgccagc ggactgtttc gagcatagga tctccgaggg ggactgggtt
     5101 cgggtcacgt gtggtgccgg tcgggtcgcg cgtcgtcagg ttgggtcgac tacccagcct
     5161 accggtgcct ggcctccgca aacagcgtca gcagccgggc cagaggcagg ccgacgacat
     5221 tatcgtacgc gccgtcgatc cggtcgatga gaaggctgcc gagcccctgg atggcgtagg
     5281 cgccggcctt gtcgcgcggc tcgccggagg cgacatagcg gtcgatgacc tccggtgtca
     5341 gggtgcggac gtggacgcgg gtcgactcca cagcggtgtg gacggtgcca cttggggtca
     5401 ccagcgccag cccggtatgt acggcatgcg gacgaccgga cagcgccccg agcatccggc
     5461 gggcgtctga gtcgtcggcg ggtttgccga gcggaacgcc gcccacgtcg acgatcgtgt
     5521 cggccgccag cacccatcga ccgggccagc gtggcgcagc cgcctcggcc ttgcggcggg
     5581 cgatcgtgag cgcctgaatg tcgggcgagt cgccggcaac gagcgattca tcggcgtcga
     5641 ccggcgccac gacatgggcg attcccgcgc gcgagaggat ctcggtccgc cgcggcgagg
     5701 ccgatgcgag gacgacgggt tcttccatcg ggacaaggag ttgcgcgagc catgcgtggg
     5761 cttcgtgcgt tgatgactct gcctgaacat agttcggtga agctgtgggt gaaacaggcg
     5821 attggaactc ggttcgaaca tagttggcca aggcaaccct ggccgcggtg gcggcagccg
     5881 actctgcctg aaccgggttc ccgccccccg cgttcgccgc ccccgactct gcctggaccg
     5941 agttccccgc ctcccgcgac tcgaccatca ccgtgaccgg tccctcgttc accagttcca
     6001 ccgacatcat ggcctggaag acgccggtgg ccacccggcg aacaccggcg gcacgcaggt
     6061 cggccacgaa gcgctcgtac agtgcattcg cggactccgg cggggcggcc tcgacgaacg
     6121 acggacggcg gccgcggtcg gcgtcgccgt acagcgtgaa ctgggagatg accagcgctt
     6181 cgccatcgac ctcgaggagg ccgaggttca tcttgccggc gtcatcctcg aatatacgga
     6241 ggccggcgca cttggtggcc agccaggcgg cctcggcgga ggtgtcaccc cggcggacgc
     6301 cgagcagtat cagcaggccc gggccgatgg cggcgtggtg atcgccggcg gtgacggagg
     6361 cgcgggagac gcgctggatc agcgcgcgca gggggctccc ctcaatgccg cgtctcgccc
     6421 tcgtcctcgc cgaggacctg atggcgctcg acggacgtga ccccgcggat gttccggatc
     6481 gccttgatga cacgttgaag gtgattcagg ttcttcacct ccaccaggaa cgtgccgagc
     6541 gcctccccat cggcggactt gatgtcggcg tttttgatgt tcgtctgcgt ctggctcacc
     6601 gcgttcgcga catcggccag gaaactctgc cggtcatccc cgtggatgat cagcttcacc
     6661 acgaacgcct tttcctccga cacgttccag ttgagctcca tccgccggtc cttccccacc
     6721 ttctcctcga agccattggg gcagtcgatc cggtgcacgg aaagcccgcg gccgcgcgtc
     6781 accacgccga tcacctggtc gcccggcacg ggctggcagc atcgggcgaa gacgatcatc
     6841 aaattgccga cgtcctggat ggagattccc ttgccgccgc cgaccgcgaa gtcgcggatc
     6901 ttcgacaacg ccgtcggcgc cggctcgcgc ttctggatga tctcggggaa gcagcgcgtg
     6961 agcacgttat gggccccggt gtgcccctct cccaccgagc gcagcaaccg ttccacgtcg
     7021 gggaagccga acgactgggc cacgtcgatc agctcccggt ccgacgggag cttcttgtgg
     7081 aagcggcgcc cctcgcgctc gagcatgtcg cgcccgagct cgatggagtg ctccatccgc
     7141 tgctcggtga gccagcggcg aactttctga cgggcgcggc tcgacttgac gatcgagagc
     7201 cactcgtccg acggatgcgc gttgggcgag gtgatgatct gcaccgagtc gccgttcttg
     7261 agtgcgtact tga
//
LOCUS       DASVOD010000292         8171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_124811, whole genome shotgun sequence.
ACCESSION   DASVOD010000292 DASVOD010000000
VERSION     DASVOD010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124811"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1339)
                     /locus_tag="VF720_06540"
     CDS             complement(65..1339)
                     /locus_tag="VF720_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879049.1"
                     /translation="MQRKALLGVGLVAVGAIVFFVLRNYPPVTNGTEGTIGAAKRYQT
                     EQIKAADVQLGDASVNDVLQSGMFHKMMTNEDFRKLVMSDGFRNAAADDGFSRMVADA
                     GARNVLNSGDVQRAAAPEGDLGKLLASDAALLVTTADGQRLIADDSFNKVLADAGARA
                     TLQDALGRRFAEAGKWAASDAGKRQIEAARSAAIESNKSMAEAGRVSMGDASKQAVEA
                     GKVSMADAGKASMSDASKQTVEAAKVSMTDAGKVSMSDAGKTSVEAARMTMSDAGKVA
                     AEAERRVMEAARMATIDGGKLGADADLAKAIKLNPELGKLVASDAFKNLASTGKLGTV
                     LGDDGLARAARSQDVAALVARPEVARVMVEYGKYLPELGKTGRVPELGRMMSDASFVQ
                     AFSDPGFGKSLAAVDGSRLNVMAEQMARNVHE"
     gene            complement(1490..2284)
                     /locus_tag="VF720_06545"
     CDS             complement(1490..2284)
                     /locus_tag="VF720_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879050.1"
                     /translation="MNDRLEQDMHDVIDGTAVEEQSLRVRSALAQDEEARRRYREMEE
                     LHERLARAPRYPAPAEIRPAVLRAIHASVGPPPTTFWSEFRESISRRPAVGMAWAFVT
                     GAMLVLAVLSLLRGPSLLPGSSLDGEGAERVAGTMVRNGEASGFREVSQGRLELPGGT
                     ALARVLQSRNVLRVELSADIAGETNLILSYPDTKRVLTRIIPDGPANIRVDMAPGRVA
                     WHSPTAGAIALEFSVDGVEDPFALESLVDGVARRISLATQGGMEGR"
     gene            complement(2281..2823)
                     /locus_tag="VF720_06550"
     CDS             complement(2281..2823)
                     /locus_tag="VF720_06550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7879051.1"
                     /translation="MDSDELIVRRVLDGHTGAFELLVDRYQTVLFNLALRILGDHDDA
                     RDVVQTAFMKAFQKLDSFCPEHRFFSWIYRIGMNESLNLRKRRRPMESIDGRIMDPAR
                     DPAAQFAEGGRRDTVQAALMQLTLDYRTVVVLRHFADLSYQEIGAMLGVPEKTVKSRL
                     FTARRLLEEYLVRKGVAQQP"
     gene            3060..3347
                     /locus_tag="VF720_06555"
     CDS             3060..3347
                     /locus_tag="VF720_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7879052.1"
                     /translation="MSFKIVPTRGGDLQITGRFDAAQVEPARKILWHLKESTRVDLSK
                     LEYISSAGIGVLVEAFSRLKSAGHTMQLVNANSMVRSVFKLTGLDRVIPLE"
     gene            3441..3884
                     /locus_tag="VF720_06560"
     CDS             3441..3884
                     /locus_tag="VF720_06560"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HEX7879053.1"
                     /translation="MPRTLCDDKDRAELLERLRRLQPDSPRQWGTLDAPGMLCHVTDA
                     FRLALGQIQSRPHYNALSRSIGKFLVVNTSLRPPKGRIKAPPETMFTSPSEWSRDLTS
                     CEELAMRVASGEASAVHPAFGPLTPEEWGRMCWKHTDHHLRQFGV"
     gene            complement(3951..4493)
                     /locus_tag="VF720_06565"
     CDS             complement(3951..4493)
                     /locus_tag="VF720_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879054.1"
                     /translation="MVIYLVSAAAPPVSGKSPSGKARVPKIPPRREYPHRLKIDREYD
                     RFEKKTVTRIEPHLLGNRLELAAFFSTPDSDWVVPASVTLIVWSEGKSWEYLKCHTFH
                     LLGDNNPIDVVAVTHDGEVVEQGVTENIVVILETANFLRWVNSEKAEFRLCRTEYLMK
                     PEEHVALIDFASRMLPPDPK"
     gene            complement(4624..5898)
                     /locus_tag="VF720_06570"
     CDS             complement(4624..5898)
                     /locus_tag="VF720_06570"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7879055.1"
                     /translation="MTPTFAALQHRNFRLLWLGQLISFSGSMMQNAAILWHVSLLAPQ
                     DQKGLYLGMVGLVRVAPIIIFSLISGVVADAVNRRKLMLFTQLIMAGIAGTLAYLTFK
                     GLTTVWPVYLLAALSSSVAAFDGPARQSIVPDLVPIEHLANAISLNSVMFQLAAVLGP
                     TLAGLVLATLDVGWAYALNGVSFLAVVGALLLMRNVPAAVDREGRGDISLGAAKEGLR
                     FVFSNPIIRSTMLLDFFATFFSSATALLPIFAQDILHVGAHGYGILYAAPSIGAFIAG
                     AAMINYVERIEHRGRTIIWAVVGYGIATVLFGLSKNFWLTFLCLALTGVADTISTVLR
                     NVVRQLVTPDALRGRMTSINMMFFMGGPQLGELEAGLVAQGFGAPVSVITGGIACLLV
                     TFGIVKKTPELAEYRKSTPVAAGDGLVSVTTS"
     gene            complement(5895..6836)
                     /locus_tag="VF720_06575"
     CDS             complement(5895..6836)
                     /locus_tag="VF720_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX7879056.1"
                     /translation="MPDDPGKKLPFPVVFHPDYDLNLGAHVFPSVKYTRIHRALLDRG
                     IAAPADILAPEPATDDDLKLVHTDDWVSRLKALKLTHAEVARLEIPVSPELVRALWLA
                     TGGSIRAGREALACGLAYNIGGGLHHAFPGHGEGFCAIHDVAVAIRKLQAEKRIQRAM
                     VIDVDVHHGNGTAAIFQGDASVFTLSIHQFNNYPFEKPESDLDIHLTDGMKDADYLRR
                     LGDGLTASLDAFQPDLAWYVAGADPYEHDRLGGLRITMNGLKERDRLVLGELRRRGIP
                     TAIALAGGYAEAVDDTVAIHVNTLIAAQEIAGAVPAA"
     gene            complement(6838..>8171)
                     /locus_tag="VF720_06580"
     CDS             complement(6838..>8171)
                     /locus_tag="VF720_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX7879057.1"
                     /translation="DSEAMVQTYREDLDKQQAVGKSMTTAPKLHELPDYWKSYTGGAW
                     HPELEVDTGLAADRIAKLAEVIGAWPEGFHIHPKVKKGLELRREMGQGKRPIDWGMAE
                     ALAFGSLLVEGSPVRLSGQDCRRGTFNHRQAVLIDIEDESEYTPLDHLAAGQARLSIY
                     DSPLSEASVLGFEYGYSRDYPEALVLWEAQFGDFVNGAQVIIDQFLSASEDKWHLLSG
                     LVMLLPHGYEGQGPEHSSARLERFLQLAAECNMQVCQPTTGAQYFHLLRRQSMRSWRK
                     PLIVMTPKSLLRHPGAASPIEQFTSGRFQTVLDDPAVTDAERLILCTGKVYFELEAER
                     MKRGAMKTALLRLEQMYPFPEEALTEVIARHPNAREVVWVQEEPGNMGALTYVAPRLG
                     RCAGGRPVRTIKRSASASPATSSQKAHNLEQAALLAHAFLEVNAAASTVAR"
BASE COUNT         1467 a         2658 c         2587 g         1459 t
ORIGIN      
        1 ggagtggtcg cacgggcttg ccgtgcgacc tctccggact actgtggggg atggttaccg
       61 agactcattc atgaacattg cgggccatct gttccgccat cacgttgagg cggctgccat
      121 ccaccgccgc caacgacttg ccgaaaccgg gatcgctgaa cgcctgcaca aagctggcat
      181 ccgacatcat ccgacccaac tcgggcacac gaccggtctt gccgagctcc ggcaggtact
      241 tcccatactc gaccatgacc cgggccacct cggggcgcgc caccagggcg gccacatcct
      301 ggctccgggc cgcgcgggcc agcccgtcgt cgccgaggac ggtgccgagc ttgccggtgg
      361 atgcgaggtt cttgaaggca tcgctcgcca cgagcttgcc cagttccggg ttcagcttga
      421 tcgccttggc aaggtcggca tcggcgccca gcttcccgcc gtcgatggtg gccatccggg
      481 ccgcttccat cacgcgacgt tccgcttcgg ccgccacctt gccggcgtca ctcatcgtca
      541 tccgggcggc ttcgaccgac gtcttgccgg catccgacat cgataccttg ccggcatctg
      601 tcatcgacac cttggcggct tcgacggtct gcttgctcgc atcgctcatc gacgccttgc
      661 ccgcatcggc catcgacact ttgccggcct cgaccgcctg cttgctcgcg tcgcccatcg
      721 acaccctgcc ggcttcggcc atcgacttgt tgctctcgat ggccgccgag cgggcagctt
      781 cgatctggcg cttgccggcg tccgaggccg cccacttacc tgcttcggcg aaccggcggc
      841 ccagggcatc ctggagggtg gcacgggcgc cggcgtcggc aagcaccttg ttgaagctgt
      901 cgtcggcgat gaggcgctgg ccgtcggcgg tggtcaccag gagcgcggca tcggaggcga
      961 ggagcttccc gagatctccc tcgggagcgg cggcccgctg cacatcgccc gaattcagca
     1021 cgttcctggc acccgcgtcc gccaccatgc ggctgaagcc gtcatcggcc gcggcgttgc
     1081 ggaaaccgtc gctcatgacg agcttccgga agtcctcgtt cgtcatcatc ttgtggaaca
     1141 ttcccgactg gagcacatcg ttgactgagg cgtcgcccag ctgcacatcg gccgccttga
     1201 tctgctcggt ctggtagcgc ttggcggcgc cgatggtccc ctcggttccg ttggtgaccg
     1261 gcgggtaatt cctcagtacg aagaacacga tcgcaccgac ggccaccaat cccacaccaa
     1321 gcaaggcttt gcgttgcacg attgaaccct cctctaatga atcgactgaa tgactccccg
     1381 tgagacgtga cgcacctgga aatacagtcg tccgtcgccg gagttggtga tcgtgtgcgg
     1441 agggagagaa tcggtgcgac atcccacgac gggagcgtcg gcgccgacct cagcggccct
     1501 ccattccacc ctgggtggca aggctgatac ggcgcgccac cccatccacg agagattcga
     1561 gcgcgaacgg atcctccaca ccgtcgacgc tgaactcgag tgcgatcgcc cccgccgtgg
     1621 gggaatgcca cgcgacgcgg ccgggggcca tgtcgacgcg gatattggca ggaccatccg
     1681 ggatgatccg agtgaggaca cgtttcgtgt ccgggtacga caggatgagg ttcgtttccc
     1741 cggcgatgtc cgccgagagc tcgaccctga ggacatttcg cgattggagg acacgcgcca
     1801 gcgcggtgcc tccgggcaat tccagccgcc cctggctgac ttcgcggaaa ccggatgctt
     1861 ccccgttacg caccatcgtg ccggcgacgc gttcggcccc ctccccgtcg agcgagctgc
     1921 ccgggagcaa cgaggggccc cgaagcaatg aaaggaccgc caggaccagc atggccccgg
     1981 tcacgaacgc ccatgccatc ccaacggccg gccgccggga aatcgactca cgaaattccg
     2041 accagaacgt tgtgggtggg ggcccgaccg aggcatggat cgcccgaagc accgccggcc
     2101 ggatttcggc cggcgcggga tagcgcgggg cccgggcgag ccgctcatgc agctcttcca
     2161 tctcccggta gcgccgccgg gcctcctcgt cttgcgcgag ggccgaccga acccggagcg
     2221 attgctcctc gacagcggtt ccgtcgatga cgtcgtgcat gtcctgctcg agccggtcgt
     2281 tcatggctgc tgcgcgacac ctttccggac gaggtactcc tcgaggagcc gtcgtgccgt
     2341 gaagagacgt gacttgaccg tcttttcggg aacgccgagc atcgccccga tctcctggta
     2401 cgaaaggtcg gcgaagtgcc ggagcaccac gaccgttcga tagtcgagcg tcagttgcat
     2461 caaggccgcc tgcacggtat ccctccttcc accctccgcg aattgcgccg cgggatcccg
     2521 ggccggatcc atgatccgac cgtcgatgga ttccatcggg cgccgcctct tccggaggtt
     2581 cagcgattca ttcatcccga tccggtagat ccagctgaag aatcggtgtt cggggcagaa
     2641 tgaatcgagc ttctggaaag ccttcatgaa ggccgtctgg accacgtcgc gggcatcgtc
     2701 gtggtcgccg aggatccgga gcgcgaggtt gaagaggacg gtctggtagc ggtccaccag
     2761 gagctcgaac gctccggtgt gcccatccag cacgcgccgg acgatcaact catcgctgtc
     2821 catggctacg gacagcgacg gtcgagaaaa gttggtcgca aacggaccct tcctgggcag
     2881 gaggctgaca gtccaacacg atgacgtgtg cctgtaaaga cgtttcgcgc gccttgtcgc
     2941 acccaactgc aattcggctt ccgccgttcc atgccgcctg ctagtctgcg gccacggcgt
     3001 cgaacggcgc caacgccgga actaaccgga ttccccaact cgttgacagg tggccgccta
     3061 tgtcattcaa gatcgttccc accagggggg gagacctcca gatcaccggg cgctttgacg
     3121 ccgcacaagt ggagccagcg agaaaaatcc tgtggcactt gaaggaatcg acccgggtgg
     3181 acctgtcgaa gctggaatac atctccagcg ccggcatcgg cgtgctcgtc gaagccttca
     3241 gccggctcaa gtcggccgga cacaccatgc agctcgtcaa cgccaacagc atggtacgca
     3301 gcgttttcaa gctgacgggc ctcgaccggg tgattcccct cgagtaatgc gcgtttcagg
     3361 cgttcaacgc gtcagtccag atgaatcgga ctcaaaacat tcaaaacgac caatcggtat
     3421 cacacgatag gagagtcgcg gtgcccagga ctctatgtga cgacaaggac cgcgccgagt
     3481 tgctcgaacg gctccggcga ctgcaacccg actcaccccg gcagtggggc acgctcgatg
     3541 ccccggggat gctctgtcac gtgactgacg cgtttcgcct ggcgctcggt cagatccaat
     3601 ccaggcctca ttacaatgcg ttgtcccgga gcatcggcaa attcctggtt gtgaacacgt
     3661 cgctgcggcc gcccaaggga agaatcaagg cgcctcccga aacgatgttc acgagcccct
     3721 cggagtggtc ccgggacctg acgagctgcg aggagctggc gatgcgggtt gcctcgggcg
     3781 aggcgagcgc cgttcatccg gcattcggtc cgctcacgcc ggaagaatgg ggccgcatgt
     3841 gctggaagca cacggaccat catttgaggc agttcggggt ttaggcgttt tgggccgatt
     3901 tttgtctcga tcccctactt gcctacgtca ggctgcgtgc cttttcacac tcatttcgga
     3961 tctggcggca acatgcggct cgcgaagtca atgagcgcaa catgctcctc aggtttcatc
     4021 aaatactccg ttctacacaa acgaaactcg gccttctctg agtttaccca ccgcagaaaa
     4081 ttcgcggtct ccagaatcac cactatgttt tctgtgaccc cttgttcgac cacttcgcca
     4141 tcatgcgtga cggccaccac gtcaatcgga ttattgtcac caaggagatg aaacgtatgg
     4201 cacttcaagt actcccacga cttcccttct gaccatacga tcaaggtgac cgaagctggc
     4261 accacccaat ccgaatcagg cgtcgaaaag aatgcggcaa gctccagtcg attcccgagt
     4321 agatgaggtt caattcgcgt cacggtcttt ttctcaaatc tatcgtattc gcggtcgatc
     4381 ttcagcctgt gaggatactc gcgtctcggt ggaatctttg gaactcgagc ttttccagac
     4441 ggactcttcc cactaactgg cggagccgcg gcagacacca ggtaaatgac catgcatatc
     4501 acctgccaag tcaacttcac tcgaatccca cccctaaaag gaagcccatg agtatcttcc
     4561 ccaatcctat agctgagcag ccgatacttc cagcggatga tataaatgct gaactggtac
     4621 cagctatgag gtggtcacgc tcaccagccc gtcccccgcg gctaccggag tcgatttccg
     4681 gtactcggcg agctccggcg tcttcttcac gatcccgaag gtcaccagca ggcaggcgat
     4741 gccaccggtg atgacggaca ccggtgcgcc gaacccttgg gccacgagtc cggcttccaa
     4801 ttcgccgagc tgcggcccgc ccatgaagaa catcatgttg atgctggtca tgcggcctcg
     4861 cagtgcgtcg ggggtgacca attggcggac gacgttccgg aggacggtcg agatcgtgtc
     4921 ggccacgccg gtgagggcca ggcagaggaa cgtcagccag aagttcttcg agaggccgaa
     4981 gagcaccgtc gcgatgccgt agccgacgac cgcccagatg atggtgcggc cgcggtgctc
     5041 gatgcgctcc acgtagttga tcatggcggc gccggcgatg aaggcgccaa tcgagggagc
     5101 agcgtacagg atgccgtagc cgtgggcgcc gacgtgcagg atgtcctggg caaagatggg
     5161 caagagcgcg gtggctgatg agaaaaacgt ggcgaagaag tcgagcagca tggtgctgcg
     5221 gatgatgggg ttcgagaaaa caaaccggag cccttccttc gccgctccca ggctgatgtc
     5281 gccgcgccct tcgcgatcca ccgcggcggg tacgttgcgc atcaacagaa gcgcgcccac
     5341 caccgccagg aatgacacgc cgttgagtgc gtaggcccag ccgacatcca gcgtcgccag
     5401 gacaagcccg gccagcgtgg ggcccagcac ggcggcgagc tgaaacatca ccgaattcaa
     5461 actgatggcg ttggcgagat gctcgatcgg cacgaggtcg ggcacgatcg actggcgcgc
     5521 ggggccgtcg aaggccgcca ccgatgacga cagcgcggcc agcaggtaga cgggccatac
     5581 ggtcgtgagc cccttgaacg tgaggtaggc cagcgttccg gcgatgccgg ccatgatgag
     5641 ctgcgtgaag agcatcagct tgcggcgatt gaccgcgtcc gccacgacgc cggaaatcag
     5701 cgagaagatg atgatcggcg ccacgcgcac gagacccacc atgccgagat acagcccctt
     5761 ctggtcctgc ggcgccagca ggctgacatg ccacaggatg gccgcgttct gcatcatcga
     5821 gccggagaac gagatgagct ggccgagcca gaggagtcgg aagttgcggt gctggagggc
     5881 ggcgaaggtg ggggtcatgc cgccggaacg gctccggcga tttcctgggc ggcgatgagc
     5941 gtgttcacgt ggatggccac ggtgtcgtcc actgcctcgg cgtagccacc ggccagcgcg
     6001 atggccgtgg ggatgccgcg gcggcgcagt tccccgagca cgagccggtc gcgctccttg
     6061 agaccgttca tcgtgatccg gagcccgccg agccggtcat gctcgtacgg atccgcaccg
     6121 gcaacgtacc aggccaggtc cggctggaag gcatcgaggc tggcggtgag gccgtcgccg
     6181 aggcggcgga ggtagtccgc gtccttcatg ccgtcggtca ggtggatgtc gaggtcggat
     6241 tccggcttct cgaacgggta gttgttgaac tggtggatgg agagggtgaa gacgctcgca
     6301 tccccctgga aaattgccgc ggtgccgttg ccgtggtgca catccacatc gatgaccatg
     6361 gcccgttgga tgcgcttttc ggcctggagt ttccgtatgg ccaccgccac gtcgtgaatc
     6421 gcgcagaaac cttcgccatg cccgggaaac gcgtggtgca gccccccgcc gatgttgtag
     6481 gccaatccgc acgccagcgc ctcgcgtccg gcccgaatgg acccccccgt ggccagccag
     6541 agcgcacgga ccagttcggg gctgaccgga atctcgagcc gggcgacctc ggcatgggtg
     6601 agcttgaggg ccttgagccg cgacacccag tcgtccgtgt ggacgagttt caggtcgtcg
     6661 tcggtggccg gctcaggagc caggatatcg gccggcgcgg cgatgccccg atcgagcagc
     6721 gcccggtgga tccgggtgta cttgaccgac ggaaagacgt gcgcgccgag attgaggtcg
     6781 tagtcggggt gaaagacgac cgggaaggga agctttttcc cggggtcgtc aggcacgtca
     6841 gcgcgcgacg gtcgaggcgg cggcgttcac ctcgaggaag gcatgggcca gcagggcggc
     6901 ctgctccagg ttgtgcgcct tctggctgct ggtggccggg ctggcgctgg ccgagcgctt
     6961 gatggtgcgg acgggtcggc ctccggcgca acggcccaga cggggcgcca cgtaggtcag
     7021 cgcgcccatg ttgccgggtt cttcctgtac ccacaccacc tcgcgcgcat tggggtgccg
     7081 cgcgatgacc tcggtgagcg cttcctccgg gaagggatac atctgctcga gccgcagcag
     7141 cgcggtcttc atggccccgc gtttcatccg ctcggcttcc agctcgaagt agaccttgcc
     7201 cgtgcagaga atgagccgtt cggcgtccgt cacggcagga tcatccagca ccgtctggaa
     7261 tcgaccactg gtgaattgct cgatcggcga ggcggcgccc gggtggcgca gcagactctt
     7321 gggcgtcatc acgatcagcg gcttccgcca cgagcgcatc gactgccggc ggagcaggtg
     7381 gaaatactgc gcgccggtgg tcggctggca cacctgcatg ttgcactcgg ccgcgagctg
     7441 caggaagcgc tcgagccgcg cgctggagtg ctccgggccc tgcccctcgt agccgtgcgg
     7501 caggagcatc acgagtcccg aaaggaggtg ccacttgtct tcggaggcgc tcaggaactg
     7561 gtcgatgatg acttgcgccc cgttcacgaa gtcgccgaac tgggcctccc agagcacgag
     7621 cgcctcggga tagtcgcggc tgtagccgta ctcgaagccg agcacgctgg cctcggaaag
     7681 ggggctgtcg taaatcgata gccgcgcctg cccggccgcc aggtgatcga gcggcgtgta
     7741 ttcgctctcg tcctcgatat cgatgaggac cgcctggcga tggttgaacg taccgcgccg
     7801 gcaatcctgc cccgacagcc gcaccggact tccctcgacg agcaggctgc cgaacgccag
     7861 cgcctccgcc atcccccagt caatcggtcg cttcccctgc cccatttcgc ggcggagctc
     7921 cagccccttc ttcaccttgg ggtgaatgtg gaacccctcc ggccacgcgc cgatgacctc
     7981 cgccagtttc gcgatgcgat ccgcggcgag ccccgtatcc acttcgagct ccggatgcca
     8041 cgcaccgccc gtgtaggact tccagtaatc aggcagttca tgcagcttgg gcgcggtggt
     8101 catgctcttc cccaccgcct gctgcttgtc gagatcctcg cggtaggtct gcaccatcgc
     8161 ttccgagtcg g
//
LOCUS       DASVOD010000293         2155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_177135, whole genome shotgun sequence.
ACCESSION   DASVOD010000293 DASVOD010000000
VERSION     DASVOD010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177135"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1615
                     /locus_tag="VF720_06585"
     CDS             <1..1615
                     /locus_tag="VF720_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7879058.1"
                     /translation="VQRSNASAMYGNASGGVIDLQSNTRFDSPFLTLRSRGGSFGLFK
                     QNIQFGAAIDKSRLTTSITGTTFDAWREHSSTQAFLLQSRLASPLSDRTNMLVMLDAT
                     ADEFYYPGALTPAQADSAPEQANPTYVSRNERRQNQVGRLAFKVDHEVSQSDDFTATL
                     FLEPKVLHRSERNRFRDFNRYHVGGSFLWRKQLAEAKPTRGTVSAGLDEAWQDGSILF
                     YNLTPEGNRGTNNVANQREGANSAGAFLQGEMVFNNVWNARASVRYDDVWYLSENRME
                     PQLNDDKHFTHVTPRVSLGRIIGQQNIYASVAGGLESPAFNEIDPPAPYDTLTSLNPI
                     LDPTYSTTFEIGMRGRAADGLNYDVSGYFITVKNDLVPIDGGVTWETAGETERKGVEG
                     SLEWQPIDRLSITGMGSVSSNEYVQYENERGDFAGNQVAALPEWFGSGLVEFKLTRGL
                     KVHGSVESVGMLFADDANTQEADAWTIGNLGADFTTLVGGTTLRLFGLVQNVTDASYT
                     ASVFINGVDGRYIEPGVERNLTLGLTLGW"
     gene            1647..1847
                     /locus_tag="VF720_06590"
     CDS             1647..1847
                     /locus_tag="VF720_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006617091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HEX7879059.1"
                     /translation="MNDRSGGEEFQKLAGSRRTGFLAEFFGFLRQNRKWWMLPMLVVI
                     LLLGALVLLGGTPLAPFIYTLF"
     gene            1852..>2155
                     /locus_tag="VF720_06595"
     CDS             1852..>2155
                     /locus_tag="VF720_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879060.1"
                     /translation="MTGAGRPHSPAAVAKRRAPSGAPAFSLPPTAWLLLAIVASFLLN
                     AGALNHPWISDDQVVLADNPTMRRTDPVTPFRILALDYWSALDADGRPFQLVGDRNL"
BASE COUNT          384 a          750 c          661 g          360 t
ORIGIN      
        1 agtgcagcgc agcaacgcgt cggccatgta cggcaacgcg tcgggcggcg tcatcgacct
       61 gcagtcgaat acccggttcg acagtccgtt cctcacgctc cgttcccgcg gcggctcgtt
      121 cggcctcttc aagcagaaca tccagttcgg cgccgcgatc gacaagtcgc gcctgaccac
      181 ctcgatcacc ggcaccacgt tcgacgcctg gcgcgagcac agcagcacgc aggcgtttct
      241 gctccagtcg cgcctggcct cgcccctcag cgacaggacg aacatgctgg tcatgctcga
      301 cgccaccgcc gatgagttct actaccccgg cgcgctcacg ccggcccagg ccgactcggc
      361 tccggagcag gccaatccga cctacgtgag ccgcaacgag cggcgccaga accaggtcgg
      421 ccggctggcc ttcaaggtgg accacgaagt gagccagagc gatgacttca ccgccacgct
      481 cttcctcgag ccgaaggtgc ttcatcgttc ggagaggaac cgattccgcg acttcaaccg
      541 ctatcacgtg ggcggcagct tcctctggcg caagcagctg gccgaggcca agccgacccg
      601 tggaacggtg agcgcaggcc tcgacgaggc ctggcaggac ggctcgatcc tcttctacaa
      661 cctcacgccc gaaggaaacc gcggcaccaa caacgtggcc aaccagcgcg agggagccaa
      721 cagcgccggc gctttcctgc agggggagat ggtgttcaac aacgtctgga atgcccgcgc
      781 ctcggtccgc tacgacgacg tctggtacct ctccgagaat cggatggagc cccagctcaa
      841 cgacgacaag cacttcaccc acgtgacgcc gcgcgtgtcg ctcggtcgga tcatcggcca
      901 gcagaatatc tacgcgtcgg tggccggggg gctcgaatca ccggcgttca acgaaatcga
      961 tccgcccgcc ccgtacgaca cgctcaccag cctgaacccc atcctcgacc cgacctactc
     1021 caccacgttc gagatcggaa tgcgcggccg cgccgccgac gggctcaact acgacgtctc
     1081 cggctacttc atcaccgtga agaacgacct cgtgccgatc gacggcggcg tcacctggga
     1141 aaccgccggt gaaaccgaac ggaagggcgt ggagggatcg ctcgagtggc agccgatcga
     1201 ccgtctctcg atcacgggga tgggttcggt gtcatccaac gagtatgtcc agtacgagaa
     1261 cgaacggggt gacttcgccg ggaaccaggt ggccgcgctt ccggaatggt tcggctccgg
     1321 cctcgtggaa ttcaagctga cgcgaggcct caaggtacac ggcagcgtgg agtcggtcgg
     1381 catgctcttc gcggacgacg ccaataccca ggaagccgac gcgtggacga tcgggaacct
     1441 gggcgccgac ttcacgacgc tcgtgggtgg aaccaccctc cgcctcttcg gcctggtgca
     1501 gaacgtcacg gacgccagct acaccgcctc ggtcttcatc aacggcgtgg acggccggta
     1561 catcgagccg ggcgtggaac ggaacctgac gctggggctt actctgggct ggtaaagggc
     1621 cccccggggc cgataaactg gttgccatga acgaccgatc cgggggagag gaattccaga
     1681 aactggctgg atcccggcgt acggggttcc tggccgaatt tttcgggttc cttcgccaga
     1741 accgcaagtg gtggatgctt cccatgctgg tggtcatcct gctgctcggt gcgctggttc
     1801 tcctgggcgg gacgccgctc gcccccttca tctacacgct tttctagctg gatgacgggg
     1861 gcgggccggc ctcattcacc cgctgcggtc gcgaaacgcc gggcgccttc gggcgcgccg
     1921 gcgttttcac ttccccccac ggcatggctc ctcctggcca tcgtggcgtc gttcctcctg
     1981 aacgccggcg ctctcaacca cccctggatt tcggatgacc aggttgtcct ggccgacaac
     2041 cccacgatgc gccggaccga ccccgtcact ccgttccgga ttcttgcgct cgactactgg
     2101 tcggcactcg acgccgacgg ccggccgttc cagctggtcg gcgatcgcaa cctct
//
LOCUS       DASVOD010000294         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_593537, whole genome shotgun sequence.
ACCESSION   DASVOD010000294 DASVOD010000000
VERSION     DASVOD010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593537"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VF720_06600"
     CDS             complement(<1..520)
                     /locus_tag="VF720_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX7879061.1"
                     /translation="MIRVALRWMVLVVALVAMAGLAVPGARAADVGVEAEVDRTEVAI
                     DGQVTYSITVSGGLRQLPTPELPTLEADWTVYSAGTSRNFSFINGQVSSSATFRYILT
                     PRRPGKLTIGKASVKIGNTVYQTEPVVITVGATAGGGGGRGSSGGGAASADAVEGVGK
                     DLFVTASVDKKNP"
     gene            complement(525..1262)
                     /locus_tag="VF720_06605"
     CDS             complement(525..1262)
                     /locus_tag="VF720_06605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879062.1"
                     /translation="MVVSDRSVMLALLLAVGLPPLWAPGPAANKKGNEAWKKGEFDKA
                     AGQYARALDALPAEKKLVFNRGTALLAQKKPDEALSALVSATADPRREVKAPAFYNAG
                     NGLFDGQKLDEAIDAYKRAILADPSDEDAKFNYELATRKKKQQDQQKKDQQKNQDKNK
                     QDQKNDQQQKDQQQQKEQQDQNQQDQSQQPPPDQQDQQQPPKPQPAAQMSKEQAEQLL
                     DALKANEKEMIKARMTSKRKRDVDKDW"
     gene            complement(1249..>1626)
                     /locus_tag="VF720_06610"
     CDS             complement(1249..>1626)
                     /locus_tag="VF720_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879063.1"
                     /translation="GVVVHTIGVGTPSGEPIPERNEDGSVGGYKKDRTGEVVLSKLDE
                     STLRDIASATGGDYFPATLAGSEVESIVRRLEGMDQQEMQAGLRTQYEERFQFFAGLG
                     ILFLLGSFVMPSGGRRKEWRGRF"
BASE COUNT          222 a          565 c          514 g          325 t
ORIGIN      
        1 acgggttctt cttgtcgacc gaggccgtga cgaaaaggtc cttgccgacc ccttccaccg
       61 catcggccga ggccgctccc ccgccggacg atccgcggcc gcctccgccg ccggccgtgg
      121 cgccgacggt gatcaccacg ggctcggtct ggtacaccgt gttgccgatc ttgaccgagg
      181 ccttgccgat cgtgagcttt ccgggccgcc gcggcgtgag gatgtagcgg aacgtggccg
      241 acgacgacac ctgcccgttg atgaacgaga agttgcgcga cgtgcctgcg gagtagaccg
      301 tccaatcggc ctcgagcgtc ggcaattccg gcgtcggcaa ctgccggagc ccccccgaca
      361 cggtgatcga ataggtcacc tgcccgtcga tggccacctc ggtgcgatcg acctcggcct
      421 ccacgccgac gtcggccgcc cgggcgccgg gcacggcaag gcccgccatc gccacgaggg
      481 caacgacgag gaccatccac cgcagggcga cccggatcac tccgttacca gtccttgtcc
      541 acgtcgcgct tccgcttcga cgtcatgcgg gccttgatca tttccttctc attggccttc
      601 aacgcatcga gcagctgctc ggcctgttcc tttgacatct gggcggccgg ctgcggcttc
      661 ggaggctgct gttgatcctg ttgatcgggc ggtggctgtt gggactgatc ctgctggttc
      721 tggtcctgct gctctttctg ctgctgctgg tctttctgct gctgatcgtt cttctggtcc
      781 tgcttgttct tgtcctggtt cttctgctgg tccttcttct gctggtcctg ctgcttcttc
      841 ttgcgcgtgg ccagctcgta gttgaacttg gcgtcctcat cggatggatc ggccaggatg
      901 gcccgcttgt aggcgtcgat ggcctcgtcc agcttctgtc cgtcgaacag gccgtttccg
      961 gcgttgtaga aggccggcgc cttcacctca cggcgcgggt cggccgtggc cgacacgagc
     1021 gccgagaggg cctcgtcggg cttcttctgc gccaggagcg cggtgccgcg attgaagacc
     1081 agcttcttct cggccgggag cgcgtcgagc gcgcgggcgt actgcccggc cgccttgtcg
     1141 aactcaccct ttttccaggc ctcgttcccc ttcttgttgg ccgcggggcc gggcgcccag
     1201 aggggcggca ggccgacggc caggaggagg gccagcatca ccgaacgatc agaaacgacc
     1261 acgccactcc tttcgccgcc cgccggacgg catcacgaac gaaccgagca ggaagaggat
     1321 gccgagtccg gcgaagaact ggaagcgctc ctcgtactgt gtgcgcaacc cggcctgcat
     1381 ctcctgctgg tccattccct cgaggcgcct cacgatcgac tccacctcgg aaccggccag
     1441 cgtggccggg aaatagtcgc cgccggtggc ggaggcaatg tcgcgcagcg tcgattcgtc
     1501 gagcttcgag agcaccacct cgccggtgcg gtccttcttg tagccgccca ccgatccatc
     1561 ttcattccgc tcgggaatcg gctcgccgga cggcgtgccg acgccgatgg tgtgcaccac
     1621 cacgcc
//
LOCUS       DASVOD010000295         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_802560, whole genome shotgun sequence.
ACCESSION   DASVOD010000295 DASVOD010000000
VERSION     DASVOD010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802560"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VF720_06615"
     CDS             <1..302
                     /locus_tag="VF720_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEX7879064.1"
                     /translation="ELGHDAGAIVIDEVAGMIVTYIMVPMPEATLPKLAVLGAGFVFF
                     RIFDILKPWPVAQFQNLPAGRGVVADDVMAGLYSNIALQAVVAFGLVRWVEGFAR"
     gene            299..>1519
                     /locus_tag="VF720_06620"
     CDS             299..>1519
                     /locus_tag="VF720_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX7879065.1"
                     /translation="MTDPAARGPAAVEILAIGNELLAGDILDTNFQALARALRDVGAE
                     VTRHLTLPDERDVIAIQVREAAARAGAVIVTGGLGPTPDDLTRFGVADGVGAALIRDE
                     GALETIRERFRKYGVGNMPVSNEVQAMFPEGATILDNPNGSAAGFRIDSPGGAIVFVL
                     PGPPRELIPMIAVHLVPWFASRAGTAVIGTRRIRSIGIGESALAEKIADWPFGLTDID
                     LGYYPQDPGVDLKLTARGRTPEAVAATLDAAEKALFVHLGLYIYSVEPTGEARTPLAE
                     VIGRRLLARGERLATAESCTGGLAAAMVTAIPGSSNWFDGGVVAYDNRVKIDTLRVSN
                     DLITAHGAVSEEVARAMADGIRARFGTTWGIGITGLAGPTGGTDEKPVGLVWIAIAGP
                     DGTTVQRTQHAGDRT"
BASE COUNT          241 a          509 c          535 g          234 t
ORIGIN      
        1 aggaactggg gcacgacgcc ggggccatcg tcatcgacga agtggccggc atgatcgtca
       61 cctacatcat ggttccgatg cccgaggcca cgctccccaa gctggccgtt ctcggcgccg
      121 gcttcgtctt cttccgcatc ttcgacatcc tgaagccctg gcccgtcgcc cagttccaga
      181 acctcccggc cggccgcggc gtggtggccg atgacgtgat ggcgggactc tattccaaca
      241 tcgcgctgca ggccgtggtg gctttcggac tggtgcgatg ggtggaaggc ttcgcccgat
      301 gaccgatccc gccgcgcgag ggccggcggc ggtggaaatc ctggcgatcg gtaacgagct
      361 cctcgccggc gacatcctcg acaccaactt ccaggcactg gcacgggccc tgcgcgacgt
      421 gggggccgaa gtcacccgcc acctcacgct gccggacgag agggatgtca ttgcgataca
      481 ggtccgcgag gccgcggcgc gcgccggtgc cgtgatcgtg accggcgggc tcggccccac
      541 ccccgatgac ctcacgcggt tcggcgtggc cgacggcgtc ggcgcagccc tgattcgcga
      601 tgaaggggcg ctcgagacga tccgcgaacg attccggaag tacggcgtgg gaaacatgcc
      661 ggtgtcgaac gaagtgcagg ccatgtttcc cgaaggcgcc acgatcctgg acaacccgaa
      721 cggctcggcg gcgggatttc gcatcgattc gcccggcggt gccatcgtgt tcgtgctgcc
      781 cggcccgcca cgcgaactga tcccgatgat cgcggtgcac ctggtgccct ggttcgcctc
      841 aagggccggt accgccgtca tcggaacccg ccgcatccgc tcgatcggca tcggcgagtc
      901 ggcgctggcc gaaaagatcg ccgactggcc gttcggcctt acggacatcg acctgggcta
      961 ctacccgcag gacccgggcg tggacctgaa gctcaccgcg cgcgggcgaa cgccggaagc
     1021 cgttgccgcc actctcgatg cggccgaaaa agcgcttttc gtgcacttgg gcctatacat
     1081 ctatagtgtc gaaccgacgg gggaggccag gacgccgctg gcggaagtca tcggccggcg
     1141 cttgctcgca cgcggcgaac gactggccac cgccgagtcg tgcaccggcg gactcgcggc
     1201 ggcgatggtc accgccatcc ccggttcgtc gaactggttc gacggcggcg tggtggcgta
     1261 cgacaaccgg gtgaagatcg acacgctccg cgtgtcgaat gacctgatca ccgcgcatgg
     1321 cgcggtgtcg gaagaggtgg ctcgcgccat ggccgatggg atccgtgcgc gcttcggcac
     1381 gacgtggggg atcgggatca ccggcctggc cgggccgacg ggcggcacgg acgagaagcc
     1441 ggtgggcctg gtctggatcg cgattgcggg gccggacggt acgacggtgc agcgcacgca
     1501 acacgcgggg gaccggacg
//
LOCUS       DASVOD010000296         8775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_959706, whole genome shotgun sequence.
ACCESSION   DASVOD010000296 DASVOD010000000
VERSION     DASVOD010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959706"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..852)
                     /gene="tig"
                     /locus_tag="VF720_06625"
     CDS             complement(<1..852)
                     /gene="tig"
                     /locus_tag="VF720_06625"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX7879066.1"
                     /translation="MRSRLVKVNVIEEPALKRVLEIEIPADEVDGGLREVVEEYRRNV
                     ALPGFRKGKAPLDMVRAQMADSLESEYLRKALPKAYMEALAESGLEPAAEPEIKDLQF
                     KKGEPLKFAAHIEVWPKFELSGWQGLSMVREEQEVADADIDAELDTFRQSQATWGAVD
                     RPAQGGDRVALDYWLLNAVGERGEEQQTGVIEVGGSGTPEPFNQALMGVSKGESRRVV
                     LPAVSHTTEEGVHEHPEQTFDIVVTEVREKSLPALDDAFAKLALGSENADLESLKARV
                     RLSLESRE"
     gene            complement(879..961)
                     /locus_tag="VF720_06630"
     tRNA            complement(879..961)
                     /locus_tag="VF720_06630"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(925..927),aa:Leu,seq:tag)
     gene            complement(1082..2137)
                     /locus_tag="VF720_06635"
     CDS             complement(1082..2137)
                     /locus_tag="VF720_06635"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX7879067.1"
                     /translation="MARSYRIALFPGDGIGPEVVAEGLRVLDAAEKRYGFRTERTTFP
                     WSGAHWLETRQLMPESVLDEVRTFDAVILGAIGHPKCPPGLVERAVVGGLRWGLDLYA
                     NVRPVRLYHERLTPLKGKTPADIDMIVMRENTEDLYVGLGGFFKKGTPDEVATGEMIV
                     TRKGTERVVRYAFELARSRPRKKLCLVDKANAVMAHDLWRRTFREVSAEYPDVTTEAL
                     YVDAAAMFMVQSPERFDVMVTTNMFGDILTDLGAMLQGGMGAAASGNIHPGRVSLFEP
                     IHGSAPDIAGTGTANPVATVLAVGMMLEFLGQTEAGLRIEEVVKGALTDGRLPGLDAK
                     TGLSTRQIGDLLVAGLA"
     gene            complement(2143..3348)
                     /locus_tag="VF720_06640"
     CDS             complement(2143..3348)
                     /locus_tag="VF720_06640"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEX7879068.1"
                     /translation="MNVYLVAGARTPMCEYESSKRGDGKPGGLLAGLSAIELGALAAK
                     EALKRSGVEPSDIDQSIFGNALQTSNDAIYGARHVALKAGVPNHVPALTVNRLCGSGI
                     QSIISAAEQIQLGRAKTVLAGGMENMSQAPHVIHGLRGGLRLGQGKMEDLLMAALLDP
                     YCGFFMAQTAENIAKRMEISRTEQDEYAVRSQKAAEAAYKAGTFKDEIVPVEVKQGRN
                     SHVVDQDDHMRFGTTMDDLAKLPAAFSKEGSVTAGNASGIVDGGAAVVVASEEFVKAK
                     GLKPLARLVDYSYVGVEPEVMGMGPVPAIQDVLKRQDLKLSDIDLFEINEAFAAQYLG
                     VERGLGLERDRVNVNGGAIALGHPLGATGTRITLTLALELKRRGAKRGISSACIGGGQ
                     GIALLIEAV"
     gene            complement(3382..4164)
                     /locus_tag="VF720_06645"
     CDS             complement(3382..4164)
                     /locus_tag="VF720_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011881868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7879069.1"
                     /translation="MAYELITYEVKGRIGYITLNRPKVLNALNGALMEEVNRAIGEAS
                     CNPDVGGVIVTGSGEKAFCAGADIDELRRNSPVKGVQTALRGQEILRHIELCGKPVIA
                     AINGHCYGGGCELAMACTLRIASDNAKMGQPEVNLGIIPGYGGTQRLPRLVGKGIALE
                     LIITGRAITAEEARQIGLVNKVVPQPELMAAAEEMLNTIMAKGPIAVRLCIEAVHRGM
                     DMDLDNGLNLEAQLFGVVASTVDMKEGLNAFLEKRPASFKNE"
     gene            complement(4166..5059)
                     /locus_tag="VF720_06650"
     CDS             complement(4166..5059)
                     /locus_tag="VF720_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX7879070.1"
                     /translation="MAIKKVGVLGCGLMGAGIAQVAAAAGYDTLVREVEAGYIDKGFG
                     RMKGMLAKGVEKGKVQQADMDATMGRLKGTTKLEELADCDIIIEAIIENLDLKRETFQ
                     TLNKVCGAETLFASNTSSLSITEMGAMSGRPDRFLGLHFFNPVPLMKLVEVVRGLNTS
                     DDTMEKGLSFAKSLGKAPIVAKDSPGFVVNLLLVPYLLGAIRAVEMGIATVPDIDQGM
                     VLGCGYPMGPLTLLDFVGLDTTYYIANIMFDEFKGAEYAAPTLLKRMVLAGYHGRKSG
                     KGFYDYSSDPPKPTTGLVMGA"
     gene            5446..5691
                     /locus_tag="VF720_06655"
     CDS             5446..5691
                     /locus_tag="VF720_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7879071.1"
                     /translation="MESIGAFDAKTRLSELLERVRKGESFVITRHGHPIARLVPESGA
                     DNEVVANAVNQLKNFHRGRPKVTLQELLDSRHGGHRY"
     gene            5691..6098
                     /locus_tag="VF720_06660"
     CDS             5691..6098
                     /locus_tag="VF720_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7879072.1"
                     /translation="MSIVVDASALLCLAFDDEAVPYGSALIDAIPRENAVSPSILWYE
                     LRNALVTSERRGRIEPELSAAFLVLTGQLPIALREPQSEAGILDLARRFKLTVYDAAY
                     LDLAILERAALATLDDGLKAAARELGVPHWQPE"
     gene            complement(6149..7222)
                     /locus_tag="VF720_06665"
     CDS             complement(6149..7222)
                     /locus_tag="VF720_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879073.1"
                     /translation="MQFVDRMSRNRKLDRLIENWIEYRDTVSRLGGQAGATPEQERQF
                     LELKASIAASLPFIAESNGSGSLNTEAQANVRGMTDLMNSQLSLASHLPGDDFLNRWH
                     THYLYLNRFKGMAQSARPMHRAGGGAAPASMPGDNYYKGDFGRRYIRPIFNNWLTRFV
                     VRLAVVAGLIILVAKVLNVDLNRAPEWGKQVASDWWGPGSGNSTSSSTNTTGGRAPAG
                     PLNAPKDTRASVNKSTRTHQDAMDSAIEGAERADGSGGGAGPVAAVKPGQGKPVSGNR
                     TVTINSGPRNTKYSPDGSNVFQPPATPGVVNDAKVVANRLVPRPVKDFFQPVTSRYGV
                     EVTVAMVGIGLLLVAYMIFGRAR"
     gene            complement(7260..8336)
                     /locus_tag="VF720_06670"
     CDS             complement(7260..8336)
                     /locus_tag="VF720_06670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7879074.1"
                     /translation="MAASILEILKEAVAEQASDILITAGSPITFHVFGQLIPYDADWV
                     LAGTETQELIYQFMTMDQRKVFENERDIDLAYHIPGLARFRVAVFQQKGTLAAVLRLV
                     PLRVPHFSEIGVAERVILEMANIPNGLILITGPTGAGKSTTIASFLEFLNTEGGNPKH
                     IISIEDPVEFLFTPKTCVIDQREIGYDVKSYVKGLRAALRQMPHVIFVGEMRDRETIE
                     VALTAAETGNVVISTLSTQSAAKTINRIIDIFPVEHQSEIRTRLALTLKVVISQVLMR
                     RIDTPGRVAAREMMFVNNAISNLIREGKVHQINNAIATGAKEGMVLMDDSILDLASRG
                     VVSYASAVIRMTDAEKLAKLKARP"
     gene            complement(8345..>8775)
                     /locus_tag="VF720_06675"
     CDS             complement(8345..>8775)
                     /locus_tag="VF720_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEX7879075.1"
                     /translation="IFVGQTIRVVTNVLVSNEGIGTTQTQIENIEVGVKLEATPRIAS
                     DGTITTLIAPEVSNILSLDPETGLPTTATRRARSLVRVASGEKIIMGGLLREESRELI
                     QKVPLLGDIPFLGYLFQHRVSRVEKTDLVIEITPSIVEGN"
BASE COUNT         1413 a         2980 c         2741 g         1641 t
ORIGIN      
        1 ttcgcggctc tccagggaga ggcggacccg ggccttcagc gactccaggt cggcattctc
       61 gctccccaag gccagcttgg cgaacgcgtc gtccagcgcc ggcagcgact tttcacggac
      121 ttcggtgacc acgatgtcga aggtctgctc ggggtgctca tgcacccctt cctcggtagt
      181 gtggctcacc gccgggagga ccacccgccg cgattctccc ttcgaaaccc ccatcagcgc
      241 ctggttgaac ggctccggcg tgccggaccc gcccacctcg atgacgccgg tctgctgttc
      301 ctcgcccctc tcgccgaccg cgttcagcag ccagtagtcg agggccaccc ggtccccccc
      361 ctgtgcggga cggtccaccg cgccccaggt ggcctgactc tggcggaagg tatcgagctc
      421 ggcatcgatg tccgcatcgg ccacttcctg ctcctcgcgg accatcgaca gcccctgcca
      481 cccggagagc tcgaacttgg gccacacctc gatgtgggcg gcaaacttga gcggctcccc
      541 cttcttgaac tggagatcct tgatctccgg ctcggcggcc ggctcgaggc ccgattcggc
      601 cagggcctcc atgtaggcct tgggaagggc tttcctcagg tactcggatt cgaggctgtc
      661 cgccatctgg gcgcggacca tgtcgagggg cgcctttccc ttgcggaagc ccggcagggc
      721 cacgttccgg cggtactcct ccacgacttc cctcaacccg ccatccacct cgtcggcggg
      781 aatctcgatt tccaggacgc gcttgagcgc gggctcttcg atgacgttga ccttgaccaa
      841 acgtgacctc atcttccttg ggagtccgga ccggaaactg gtgcgagagg ggggactcga
      901 acccccacgg ttgcccaccg gatcctaagt ccggcgcgtc tgccagttcc gccactctcg
      961 cggtgttgga ccgacgaaag cgcggtcagt atagcacacg ctgtcgatcc gctaagttaa
     1021 ttcctgccag tagcttagtg caatttgccc gagtggggcc ggtaccctgt ccgggccctt
     1081 ctcaggccag accggccacc aggagatcgc cgatctggcg ggtggaaagg ccggttttcg
     1141 catccagccc gggtagtcgg ccatcggtca gggcgccctt cacgacctcc tcgatccgga
     1201 gccccgcttc ggtctggccc aggaactcca gcatcatccc caccgccagg accgtggcca
     1261 cgggattggc cgttccggtg cccgcaatgt cgggcgccga tccgtggatc ggctcgaaga
     1321 ggctcaccct gccggggtgg atgttgccgc tggcggccgc ccccatccct ccctgcagca
     1381 tggcgcccag atcggtcagg atgtcgccga acatgttggt cgtgaccatc acgtcgaagc
     1441 gctccgggct ctgcaccatg aacatggccg ccgcatccac gtagagcgcc tcggtcgtca
     1501 cgtccggata ctcggcggaa acctcgcgga acgtgcgccg ccacaggtcg tgcgccatca
     1561 ccgcgttggc cttgtccacc aggcagagct tcttccgcgg ccgcgaccgc gccagctcga
     1621 aggcgtagcg cacgacgcgc tcggtaccct ttcgtgtcac gatcatctcg ccggtggcca
     1681 cttcgtcggg tgtccccttc ttgaagaagc cgccgagtcc cacgtagagg tcttcggtgt
     1741 tctcgcgcat gacgatcatg tcgatgtcgg ccggcgtctt ccctttcagc ggcgtcaacc
     1801 gctcgtggta gagccgcacc ggccggacgt tggcgtagag gtcgagcccc cagcgcagcc
     1861 caccgaccac ggcccgctcg acgaggccgg gcgggcactt cggatggccg atggccccga
     1921 gaatcaccgc gtcgaacgtc cggacctcgt ccagcacgct ctccggcatc agctgccgcg
     1981 tttcgagcca gtgcgccccg ctccacggga atgtcgtccg ctccgtgcgg aagccgtagc
     2041 gcttctccgc ggcgtccagc acgcggaggc cttcggccac gacctcgggg ccgatgccgt
     2101 cgccgggaaa gagggcaatg cggtaggaac gtgccacgcc ggtcagaccg cctcgatcag
     2161 gagcgcgatt ccctgcccgc cgccgatgca ggcggacgag atgccccgct tcgcgccgcg
     2221 gcgcttgagc tccagcgcca gcgtcagcgt aatgcgcgta ccggtggcgc cgagcggatg
     2281 acccagcgcg atggcgccgc cattcacgtt cacccgatcc cgctccaacc ccaggccgcg
     2341 ctccacgccc aggtactgcg cggcgaacgc ctcgttgatc tcgaagaggt cgatgtccga
     2401 cagcttgaga tcctggcgct tcaacacgtc ctggatggcc ggaaccggcc ccatgcccat
     2461 cacttcgggc tcgacgccca cgtacgaata atccacgaga cgagccagcg gcttgagccc
     2521 cttggccttc acaaactcct cggacgcaac aacgaccgcc gcgccgccat ccacgatgcc
     2581 gctggcgttg ccggccgtca ccgacccttc cttggaaaac gccgccggca acttggccag
     2641 gtcgtccatc gtggtgccga agcgcatgtg gtcgtcctgg tcgaccacgt gggaattgcg
     2701 tccctgcttc acctcgaccg gcacgatctc atccttgaag gtgccggcct tgtacgccgc
     2761 ttcggccgcc ttctggctgc gcacggcgta ttcgtcctgc tcggtgcgcg agatctccat
     2821 gcgcttggcg atattttcgg ccgtctgcgc catgaagaag ccgcagtagg gatcgaggag
     2881 cgccgccatc agcaggtctt ccatcttccc ctggccgagg cgcaggccgc cccgcaggcc
     2941 atggatgacg tgcggcgcct ggctcatgtt ttccatgccg ccggccagca ccgtcttcgc
     3001 ccgcccgagc tggatctgtt cggcggcgga aatgatcgac tgaatgcccg agccgcagag
     3061 gcggttcacg gtcaacgccg gaacgtggtt gggcacgccg gccttcaatg ccacgtgacg
     3121 cgcgccgtag atcgcgtcgt tcgaggtctg cagcgcattg ccgaagatcg actgatcaat
     3181 gtcggatggc tcaacgccgg agcgcttgag cgcttccttc gcggccaggg cccccagctc
     3241 gatcgcggaa aggccggcca gcagtccacc cggcttgcca tctccccgct tgctcgactc
     3301 gtattcacac atcggcgtcc gcgcgccggc caccaggtag acattcatga tcgtcaattc
     3361 ctcttgtcct gagacatcgt cttattcgtt cttgaacgag gcaggccgct tctcgaggaa
     3421 cgcgttgagc ccctctttca tgtccacggt cgaggccacc acgccgaaga gctgcgcctc
     3481 gaggttcagg ccgttgtcga gatccatgtc catcccgcga tggaccgcct cgatgcagag
     3541 ccgcaccgca atcggcccct tggccatgat ggtgttcagc atctcctcgg ccgccgccat
     3601 caattccggc tgcggcacca ccttgttcac gaggccgatc tgccgggcct cctcggcggt
     3661 gatcgcccgc ccggtaatga tcagctcgag cgcgatcccc ttgcccacca gccgcggcag
     3721 ccgctgcgtg ccgccatacc ccgggatgat ccccaggttc acttccggct gacccatctt
     3781 cgcgttgtcg ctggcgatgc ggagcgtgca ggccatggcc agctcgcatc ccccgccgta
     3841 gcagtgcccg ttgattgccg cgatgaccgg cttcccgcac agctcgatgt ggcggaggat
     3901 ttcctgcccg cggagcgccg tctgaacgcc cttgaccggc gaattccgcc ggagttcatc
     3961 gatatccgcg ccggcgcaga aggccttctc gcccgatccg gtgacgatga caccccccac
     4021 gtcggggttg cacgaggcct cgccgatggc ccggttcact tcctccatga gcgccccgtt
     4081 cagagcgttc aggaccttgg gccggttcag ggtgatgtag ccgatccgcc ccttcacttc
     4141 gtaggtgatc agttcgtagg ccatgtcagg ctcccatcac gaggccagtg gtgggcttcg
     4201 gcggatccga gctgtagtcg tagaacccct tgcccgactt ccgcccgtgg taaccggcca
     4261 gcaccatccg cttgagcagc gtgggcgcgg cgtactcggc acccttgaac tcgtcgaaca
     4321 tgatgttggc gatgtaataa gtggtatcga gccccacgaa gtcgagcagc gtgagcggcc
     4381 ccatcgggta accgcacccg agcaccatcc cctggtcgat gtccggcacc gtggcgatcc
     4441 ccatctcgac cgcgcggatg gcgccgagca ggtaggggac gagaagcagg ttcacgacga
     4501 accccgggct atccttggcc acgatcggcg ccttcccaag cgacttggcg aacgacagcc
     4561 ccttctccat cgtgtcatcc gaggtgttga ggccgcgcac aacctccacg agcttcatca
     4621 gcggaaccgg gttgaagaag tgcagcccca ggaagcggtc cggccgcccc gacatcgcgc
     4681 ccatctcggt gatcgagaga ctcgacgtat tcgacgcaaa cagcgtctcg gccccgcaaa
     4741 ccttgttcaa cgtctggaac gtctcgcgct tcaggtcgag gttctcgatg atggcctcga
     4801 tgatgatgtc gcagtcggcc agctcttcca gcttcgtggt gcccttgagg cgacccatcg
     4861 tggcgtccat gtccgcctgc tggaccttcc ccttctccac acccttggcc agcatcccct
     4921 tcatgcggcc gaatcccttg tcgatgtacc ccgcctcgac ctcacgcacc agcgtgtcgt
     4981 agccggccgc ggccgccacc tgtgcgattc ccgcgcccat caggccgcag ccgaggacgc
     5041 cgactttctt gattgccatg ggaaagcctc ccgatgcacc cggtcccccg ggccgtttga
     5101 agcgagtttc gatcgacgtg ctggtgcggg acacggccgg ctgacggtga ctcccggcct
     5161 gtcgtgcgga atggttcggt cacacccgaa tgaattttgg gcggcgggga gcatagcacg
     5221 accaaggaga ccgtaccggt gatgccactt tccgactcga tgcgacacgg acgactctcc
     5281 ggatgacggc aaaccgccga ggatccggcc atccagggcg cgcacccgaa gtcgaaattc
     5341 ttcacaattg aatgtcagaa aacaagttaa caggtccaac ttgccggggc acgacctctg
     5401 cgcctattat caaccggttg tcaaccagtc tggaggacac cttatatgga gtcgatcggg
     5461 gcctttgatg ccaagacgcg tctcagtgag ctgctggaac gcgtccggaa gggggagtcg
     5521 ttcgtgatca cccgacatgg ccatccgatc gcacggcttg tccccgaaag cggtgccgac
     5581 aacgaggtgg tggcgaacgc ggtgaaccag ctcaagaatt ttcaccgtgg acggccgaaa
     5641 gtgacgctgc aggaactgct cgactcgcgt cacggtggtc atcgctactg atgtcgatcg
     5701 tcgtcgatgc atcggcgctg ctctgcctgg ctttcgatga cgaagccgtg ccgtacgggt
     5761 ccgcgttgat cgatgccatc ccgcgcgaaa acgcggtgtc cccgtcgatc ctctggtatg
     5821 aactccgcaa cgccctggtc accagcgagc gccgcggccg aatcgagccg gaactctcgg
     5881 ctgcgttcct ggtcctgacc gggcaactgc cgatcgcgct tcgggaaccg caatctgaag
     5941 ccggcatcct ggatctggcc cggcgattca agctaacggt ctacgacgcg gcgtacctgg
     6001 atctggccat tcttgaacgg gcggcgctgg cgacattgga tgatgggctc aaggcagcgg
     6061 cgcgggagct gggcgtccct cattggcagc cagaatagcg ggcaaccgaa cgatccgtgt
     6121 tgttgtaacc gaccgctctg gaactcccct accgcgccct tccaaaaatc atgtacgcca
     6181 ccagcagcaa cccgatcccg accatcgcca ccgtcacttc cacgccgtag cgcgaggtga
     6241 ccggctggaa gaaatccttg accgggcgcg ggacgaggcg attggccacg accttggcgt
     6301 cgttgacgac cccgggggtg gcggggggct ggaagacgtt ggagccgtcg ggtgagtact
     6361 tggtgttgcg cgggccggag ttgatggtga cggtgcggtt gcccgacacg ggctttccct
     6421 ggccgggctt cacggccgca accgggccgg cgccgccgcc cgagccgtcg gcgcgctcgg
     6481 cgccctcgat ggcggagtcc atggcgtcct ggtgggtgcg ggtgctcttg ttgacggagg
     6541 cgcgcgtgtc tttcggcgcg ttcaacggcc cggcaggcgc tcgaccaccg gtggtgttgg
     6601 tcgaactgct cgtggagttg ccgcttcccg gaccccacca gtcgctggcc acctgcttgc
     6661 cccattcggg ggcgcggttc aggtccacgt tgaggacctt ggccaccagg atgatgaggc
     6721 cggccacgac ggccagacgc acgacgaagc gcgtcagcca gttgttgaag atgggccgga
     6781 tgtaacgacg accgaagtcc cccttgtagt agttgtcgcc cggcatgctg gcgggtgcgg
     6841 cacccccgcc ggcacggtgc atcgggcggg ccgactgcgc catccccttg aagcggttca
     6901 ggtagaggta atgcgtgtgc cagcgattga ggaagtcgtc gcccggcagg tggctggcca
     6961 ggctgagctg gctgttcatg aggtcggtca tcccgcgcac gttggcttgt gcctcggtgt
     7021 tcaggcttcc cgatccgttg ctctcggcga tgaacggcag cgacgccgcg atcgacgcct
     7081 tgagctcgag aaactggcgt tcctgttcgg gcgtggcgcc ggcctggccg ccgaggcggc
     7141 tgaccgtatc tcggtactcg atccagttct cgatgagacg gtcgagcttc cgattccggg
     7201 acatccggtc aacgaactgc atgacacacc gtccttgtca gcgccccgcc caggatttcc
     7261 taggggcgcg ctttcagctt ggccagcttt tccgcgtcgg tcatgcgaat gaccgcgctg
     7321 gcataggaaa cgaccccgcg gctggccagg tccaggatcg aatcatccat gagaaccatc
     7381 ccctccttgg caccggtggc gatggcgttg ttgatctggt gcacttttcc ttcgcggatc
     7441 aggttcgaga tggcgttgtt cacgaacatc atctcgcgcg cggccacccg gccgggcgtg
     7501 tcgatccgcc gcatcagcac ctggctgatg acgaccttga gcgtgagcgc cagcctcgtg
     7561 cggatttccg actggtgttc caccgggaag atgtcgatga tgcggttgat cgtcttggcg
     7621 gccgactgcg tggagagcgt ggaaatgacc acgttgcccg tttccgcggc ggtgagcgcc
     7681 acttcgatcg tttcccggtc gcgcatttca cccacgaaga tcacgtgcgg catctggcgc
     7741 agcgccgcgc gcaggccctt cacgtagctc ttcacgtcgt agccgatttc ccgctggtcg
     7801 atgacgcagg tcttcggcgt gaagaggaac tccaccgggt cctcgatgga gatgatgtgc
     7861 ttgggattgc ctccctcggt attcaggaac tcgaggaagc tggcgatggt ggtggacttg
     7921 ccggcgccgg tcgggcccgt gatgaggatc agcccgttcg gaatgttggc catctcgagg
     7981 atgacccgct cggccacgcc gatctcggag aagtgcggca cgcgcagggg cacgagccgc
     8041 agcaccgccg ccagcgttcc cttctgctgg aagacggcca cgcggaatcg agccaggccc
     8101 ggaatgtggt aggccaggtc gatgtcgcgc tcgttctcga acaccttgcg ctggtccatc
     8161 gtcatgaact ggtagatcag ttcctgcgtt tccgtgcccg ccagcaccca gtccgcatcg
     8221 tacggaatca gctggccgaa cacgtggaac gtgatcggcg agccggccgt gatgaggatg
     8281 tccgaggcct gctcggccac cgcttccttc aggatttcga ggatcgatgc cgccatggcg
     8341 gacctcagtt cccttccacg atcgacgggg tgatctcgat gaccaggtcg gtcttctcga
     8401 cgcggctcac gcgatgctgg aacaggtagc ccaggaaggg aatgtcgccc agcaggggca
     8461 ccttctggat gagctcgcgg ctttcctcgc ggaggaggcc acccatgatg atcttctcgc
     8521 cgctggccac ccgcaccagg cttcgcgccc ggcgggtggc ggtggtcggc aggcccgttt
     8581 cggggtcgag cgaaaggatg ttcgacactt caggggcgat cagcgtcgtg atcgtgccgt
     8641 cgctcgcgat gcgcggcgtg gcctcgagct tgacaccgac ctcgatgttc tcgatctggg
     8701 tctgggtggt cccgatcccc tcgttcgaca ccagcacgtt ggtcaccacc cggatcgtct
     8761 ggccgacgaa gatgt
//
LOCUS       DASVOD010000297         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_384869, whole genome shotgun sequence.
ACCESSION   DASVOD010000297 DASVOD010000000
VERSION     DASVOD010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384869"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2635
                     /locus_tag="VF720_06680"
     CDS             <1..>2635
                     /locus_tag="VF720_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX7879076.1"
                     /translation="NDANDSVCKFNNLPVATVVGNYPTNSVTPMGIGAGSDGNMIVSP
                     GSNSTWTKINNTTGAIMPLPGPETVGNFPYTYSDFTGAQVGISGVQQGTWNVITDAGT
                     SAHAWNFIGWNQSTPAGTSVLVEARVAQNLIVLATKPWVTIGAPGPQTPVPGRYIETR
                     VRLQRAVEGCSPPFITPILYDLTVGAICDPCAFANCPSDTTIPCTSPQGAEFFYTPPI
                     LKSICDSTWNVSCFPNSGFFPVGTTAVTCTAINNKDETVTCTFNVTVTGGCDHPPEGA
                     CCYRGNCYITDQASCEGVGGVYMGDNTNCVLNLCRQDCVVPPNNLVAWWPFNTAAGSV
                     TPNLAGPLTGGTLINAPTVVSGQKVQNAYSFNGTTQYINVPHNATLDLGGGDFTYDCW
                     IRTNQSTGVATIFDKRSSGSIQGTAFFLFSGYPSLQMAVANNFSNYILTAANGGAAAF
                     VADGNWHFIAVTVDRDDPAGIQFYVDGAAVGTPFNPVPRSGSLNNTLALLIAQRYPAL
                     GGGFFNGSLDEIEIIRRELSSSEISDLYMADVAGKCPETCYATQNATCCNGYSSSSAV
                     TICNYSLTPQTYSFGLTPLNTGVGCGPVGANGFSPASGTVTVPAQSCVTTPIYIACPS
                     QLLPGQAACYSVSVFNHNTGRFFGCTGSVRKVGKWCWKDVPIDITPVGGIHMVPSGGA
                     RVIQFMVGNMGPANPGLPPEAINYMIQPVRGDNGEPSRALSLNGLPPGEPVIGTLTLH
                     GGQSDMFSIDVAYPDEALIGYDRLVIWGDDDGDGVDERLGEVAVRNFSTAVSAVPDEP
                     SPVTGPPADASRLLLAFPNPFGASDAIKFRVDEGASVREVKLRLYDLQGRLVKAFILE
                     DLMPPGEYTVKWDTHDNRGEPL"
BASE COUNT          461 a          920 c          833 g          421 t
ORIGIN      
        1 aacgacgcca acgattcggt ctgcaagttc aacaacctgc cggttgccac ggtcgtcggc
       61 aactacccga ccaactccgt caccccgatg gggatcgggg cggggagcga cgggaacatg
      121 atcgtctccc ccggcagcaa ctccacctgg accaagatca acaacacgac cggcgcgatc
      181 atgccgctcc ccggcccgga gacggtgggc aacttcccgt acacgtacag tgacttcacc
      241 ggcgcgcagg tggggatcag cggcgtgcag cagggcacct ggaacgtgat caccgacgcc
      301 ggcacgagcg cgcatgcgtg gaacttcatc ggctggaacc agtcgacgcc ggcgggcact
      361 tcggtgctgg tggaagcgcg cgtggcgcag aacctcatcg ttttggccac caagccctgg
      421 gtgacgatcg gcgcgcccgg cccgcagacc cccgtgcccg ggcgctacat cgaaacccgg
      481 gtccgcctgc agcgggcggt ggaaggatgc tccccgccgt tcatcacgcc catcctgtac
      541 gacctcacgg tgggcgccat ctgcgatccg tgcgccttcg ccaactgtcc gtcggacacg
      601 accattccgt gcacctcgcc gcagggggcc gagttcttct acacgccgcc gattctcaag
      661 tcgatctgcg acagcacctg gaacgtgagc tgcttcccga acagcggctt cttcccggtg
      721 ggaacgacgg ccgtcacctg cacggcgatc aacaacaagg acgagacggt gacgtgcacg
      781 ttcaacgtca cggttaccgg cggctgcgac catccccccg agggtgcctg ctgctaccgc
      841 ggcaactgct acatcaccga ccaggcctcg tgcgaggggg tgggcggcgt gtacatgggc
      901 gacaacacga actgcgtgct caacctctgc cggcaggact gcgtggtgcc gccgaacaac
      961 ctcgtggcct ggtggccgtt caacaccgcg gcggggagcg tgacgcccaa cctggcgggc
     1021 ccgctcacgg gcggcacctt gatcaacgca ccgacggtcg tgagcgggca gaaggtgcag
     1081 aacgcctatt cattcaacgg cacgacccag tacatcaacg tgccgcacaa cgccacgctc
     1141 gacctggggg gcggcgactt cacctacgac tgctggattc gcacgaacca gtcgaccggg
     1201 gtggccacga tcttcgacaa gcgaagctcc ggctcgatcc agggaacggc cttcttcctc
     1261 ttcagcggct atccctcgct gcagatggcg gtggccaaca acttctcgaa ctacatcctt
     1321 accgccgcca acggcggcgc cgcggcattc gtggccgacg gcaactggca cttcatcgcc
     1381 gtcacggtcg atcgtgatga cccggccgga atccagttct acgtggatgg cgcggcggtc
     1441 ggcacgccgt tcaacccggt tccccgttcc ggaagcctga acaacacgct ggcgctcctc
     1501 atcgcccagc gataccccgc cctcggcggc ggcttcttca acggttcgct cgacgagatc
     1561 gagatcatcc gccgcgagct gtcatcgtcg gagatctccg atctctacat ggccgacgtg
     1621 gctggcaagt gccccgagac gtgctacgcc acgcagaatg ccacctgctg caacggctac
     1681 agctcgagct cggcggtcac catctgcaac tacagcctga cgccgcagac ttacagcttc
     1741 ggcctgacgc cgctcaacac cggcgtgggt tgtggcccgg tcggcgccaa cggcttttcc
     1801 cccgcctcgg gaacagtgac cgtgccggcg cagtcgtgcg tgaccacgcc gatctacatc
     1861 gcctgcccga gccagctcct gccgggacag gcggcctgct actcggtgtc ggtcttcaat
     1921 cacaacaccg ggcgcttctt cggctgcacc ggttcggtgc gcaaggtcgg caagtggtgc
     1981 tggaaggacg tgccgatcga catcacgccg gtgggcggca tccacatggt gccgagcggc
     2041 ggtgcgcgcg tcatccagtt catggtcggc aacatgggcc cggccaaccc cggcctgccg
     2101 cccgaggcga tcaattacat gatccagccc gtccgcggtg acaacggaga gccgagccgc
     2161 gcgctgtcgt tgaacggcct gccgcccggc gaaccggtga tcggcacgct cacgctgcac
     2221 ggcggccagt ccgacatgtt ctcgatcgac gtggcctatc ccgatgaagc gttgatcggc
     2281 tacgaccgcc tggtcatctg gggcgacgac gacggcgacg gcgtggacga gcggctgggc
     2341 gaagtggccg tgcgcaactt ctcgacggcg gtgtcggcgg tgccggacga gccgtcgccc
     2401 gtgacgggcc cgccggccga cgcgtcaagg ctgctcctgg ccttcccgaa cccgttcggc
     2461 gccagcgatg ccatcaagtt ccgggtggac gagggcgcct cggtccgcga ggtgaagttg
     2521 cgactgtatg accttcaggg tcgtctcgtg aaggcgttca tcctcgaaga tctcatgccg
     2581 cccggcgagt acacggtgaa gtgggacacg cacgacaacc gcggcgagcc gctcc
//
LOCUS       DASVOD010000298         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_332753, whole genome shotgun sequence.
ACCESSION   DASVOD010000298 DASVOD010000000
VERSION     DASVOD010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332753"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /locus_tag="VF720_06685"
     CDS             complement(<1..2117)
                     /locus_tag="VF720_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7879077.1"
                     /translation="MDTDRTPPLPLAGHDFDAFLARITADRRYAGQLAHVGRLPERPA
                     RHAEPSERLPEVLSRALAARGIERLYRHQAEAIDLARMGQHLVVVTGTASGKTLAYNV
                     PVLEGLMADPDATALYLFPTKALAQDQLKGLTRLLESMSATAPFGNGRDGSDDAPADL
                     IDSIRPGVYDGDTSQSARRKIRDTGRLVLSNPDMLHRALLPYHGRWSRFLSRLRYVVV
                     DEIHTYRGIFGSNVANVLRRLRRVAAHHGADPQFLSSSATIANPVELAEALTGVATRA
                     VTEDGSPRGPRWFVTWNPPHVDKARVERRSGNVEAMEILVRLVRDGVSTIVFCKARVV
                     AELIYKYAHDELRRNDGALAGRLTPYRSGYLPSERRAIEKRLFDGELLGVVSTNALEL
                     GIDIGSLDASIVVGFPGTFASVWQQSGRAGRGHQGSLTIFVAYDDPIDQYLARHPDYF
                     LGQAPEAAAIDPGNPYILAGHIACAAFELPLGPEDEELFGPLTTGVRDALEAEHQLAG
                     VGGLHYFSSTDFPAQKVNLRTISDDTYSIVDTTNGDRVIGMVDAISAPELVYPEAIYL
                     HEGETWFVKKLDMEQKVAYVEPVSVDYYTQPVLDTSLRVTERREERWSGPERLTLNRA
                     TVTWATTMFKKIQFGSTDSIGYKNLGLPPQHLDTVALGWSPSDDVRNAVRAEGRKPAE
                     GLSGIRNLMITVLPLFVMCDRSDV"
     gene            2272..2952
                     /locus_tag="VF720_06690"
     CDS             2272..2952
                     /locus_tag="VF720_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7879078.1"
                     /translation="MSERILVVDDERDLLDLVVYHLTHAGHKVTTAGNGEQALERARE
                     RTPDLIILDIMLPDMGGLEVCRILKGEERTKHVPVIFLTAKGEEIDRVVGFELGADDY
                     VAKPFSPRELVLRVRALLKRRRGDDDIRPPLAAGDLRLDRDRHEVTIGKNPIELTATE
                     FRLLAHLMENPGRALSRDNLLDSVWGTEAFVTDRTIDTHVKRLRAKLGKTGDKVETVR
                     GVGYRFRD"
BASE COUNT          501 a          961 c         1029 g          490 t
ORIGIN      
        1 acgtcgcttc ggtcgcacat cacgaagagc gggagcaccg tgatcatgag gttgcggatt
       61 ccggacaaac cctccgccgg ctttcgtcct tccgcacgga ccgcgttccg cacatcgtcc
      121 gacgggctcc acccgagggc cacggtgtcg aggtgctgcg gcggcagccc caggttcttg
      181 taaccgatcg aatcggttga gccgaactgg atcttcttga acatcgtggt ggcccaggtg
      241 acggttgccc ggttgagcgt gagccgctcc ggcccgctcc agcgctcctc gcggcgctcg
      301 gtcacgcgga gcgaagtgtc gagcaccggc tgcgtgtagt aatcgacgct caccggttcg
      361 acgtaggcca ccttctgctc catgtcgagc ttcttcacga accacgtctc cccttcgtga
      421 aggtagatgg cctcggggta gacgagctcg ggagcgctga tcgcgtcgac catcccgatc
      481 accctgtccc cgttggtggt gtcgacgatg ctgtaggtgt cgtccgaaat cgtgcgcagg
      541 ttcaccttct gggccggaaa gtcggtggaa ctgaaatagt ggaggccacc gacaccggcc
      601 agctgatgct cggcctcgag ggcgtcgcgc acgccggtgg tgaggggccc gaagagttcc
      661 tcgtcttccg ggccaagtgg cagctcgaac gccgcgcagg caatgtggcc ggccaggatg
      721 tagggattgc cgggatcgat ggcggcggct tcgggcgcct gcccgaggaa gtaatcggga
      781 tgccgggcca ggtactggtc gatcggatca tcgtaggcca cgaaaatggt gaggcttccc
      841 tggtgtcccc gccccgcgcg ccccgactgc tgccagaccg acgcaaaggt gccgggaaaa
      901 ccgaccacga tggatgcatc gaggctgccg atgtcgatcc ccagctcgag ggcattggtg
      961 gagaccacgc cgaggagctc cccgtcgaag agccgcttct cgatcgcccg gcgctccgaa
     1021 gggaggtagc cgctccggta cggcgtgagg cgcccggcca gcgctccgtc gttccgccgg
     1081 agctcgtcat gggcgtattt gtagatcaac tcggccacca cgcgcgcctt gcagaagacg
     1141 atcgtcgaaa caccgtcccg gaccaggcgg acgaggattt ccatcgcctc gacgttgccg
     1201 ctccgtcgct cgacgcgggc cttgtcgacg tgaggcgggt tccaggtgac gaaccatcgc
     1261 ggtccgcgcg gcgagccatc ctcggtgacg gcgcgcgtgg ccacaccggt aagcgcctcg
     1321 gcgagctcca ccgggttggc gatggtggcg ctcgacgaga ggaactgcgg atcggcgccg
     1381 tggtgagccg ccacgcggcg gaggcggcgc agcacgttgg ccacgttcga tccgaagatg
     1441 ccgcggtagg tgtggatttc gtcgacgacc acgtaccgaa gccgcgaaag gaaccggctc
     1501 catcggccgt gatagggaag gagcgcccga tggagcatgt ccggattcga gaggacgagt
     1561 cgaccggtat cgcggatctt gcgccgcgcc gactgggagg tgtctccgtc gtagacgccg
     1621 ggcctgatcg agtcgatcag gtcggcgggg gcgtcgtcac tcccatcccg gccgtttccg
     1681 aagggagcgg tggcggacat cgactcgagc agccgggtga gccccttcaa ctggtcctgg
     1741 gccagggcct tggtcgggaa gagatagagg gcggtggcat ccgggtcggc catcagccct
     1801 tcgaggacgg ggacgttgta cgccagagtc ttgccgctgg ccgtgccggt gacgaccacc
     1861 aggtgctggc ccatccgggc caggtcgatg gcctcggcct ggtggcgata gaggcgctcg
     1921 atcccccggg cggccagggc ccgcgagagg acctccggaa gcctttcgga gggctcggca
     1981 tgacgcgccg gtcgttcggg gagtcggccc acgtgggcca gctggccggc gtagcggcgg
     2041 tcggccgtga tccgcgccag gaaggcatcg aagtcgtggc ccgccagggg cagggggggt
     2101 gtccggtcgg tgtccattgc tcaacatatg tgtagcggat cgtgggccgg aagtccactc
     2161 attcctgcac tgtgacggtt gtcagaaagc gccggctccg ccactctttc gacaaggaat
     2221 cgccacagtc ggccgctagg ctggccccgt catcgaaccg gaggagcagc gatgtcggag
     2281 cggatactgg tggtcgacga cgaacgggat ctcctggatc tcgtcgtata tcacctgaca
     2341 catgcaggtc acaaagtcac caccgccggc aacggggagc aggcgctcga acgcgcgcgc
     2401 gagcgcacgc ccgacctcat catcctggac atcatgcttc ccgacatggg tgggctcgag
     2461 gtctgccgga tcctgaaggg agaagaacgg acgaagcacg tgccggtcat tttccttacc
     2521 gccaaggggg aggagatcga ccgcgtggtg gggttcgagc tgggggccga tgattacgtg
     2581 gccaagccat tcagcccccg cgaactggtg ctccgggtgc gggcgctcct caagcgccgc
     2641 cgcggcgacg acgacatccg tccgccgctg gccgccggcg atctccgtct cgaccgggat
     2701 cgccacgaag tgacgatcgg caagaacccg atcgaactca ccgccaccga attccgcctg
     2761 ctcgcccacc tcatggagaa tcccggacga gccctctccc gcgataacct cctcgattcg
     2821 gtctggggta ccgaggcctt cgtcaccgac cgcacgatcg acacgcacgt gaagcggctc
     2881 cgcgccaagc tcggcaagac cggcgacaag gtcgaaacgg tccgcggcgt cggctatcgt
     2941 ttccgcgact gaggccggcg ccgtccggtc gatcggccac c
//
LOCUS       DASVOD010000299        10453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_750573, whole genome shotgun sequence.
ACCESSION   DASVOD010000299 DASVOD010000000
VERSION     DASVOD010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10453
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750573"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1126)
                     /gene="ruvB"
                     /locus_tag="VF720_06695"
     CDS             complement(83..1126)
                     /gene="ruvB"
                     /locus_tag="VF720_06695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX7879079.1"
                     /translation="MDETRGITDPERLDDDSQLDSNLRPQRMDDFVGQRSLRENLGIG
                     LQAAMRRGEALEHVLFYGPPGLGKTTLSTLLARELGVEILFTSGPVLERPGDLAGVLT
                     NIPERGILFIDEIHRLNHTVEEYLYPAMEDFTLDIMIDKGPSARSVRLTLPKFTLVGA
                     TTRAGLLTAPLRSRFGMTYRLNYYDADELQVIVRRSARILDVPIDDAGAAEIAGRARG
                     TPRIANRLLRRVRDYAEVKGDGTINAPIARASLDLLEVDTKGLDVMDRRLLEALIQKF
                     AGGPVGLNTLAMAVGEEADTMEDVYEPFLVQEGFLERTPRGRMATELAYKHLGIQPNR
                     ARNASGGQENLFS"
     gene            complement(1182..1838)
                     /locus_tag="VF720_06700"
     CDS             complement(1182..1838)
                     /locus_tag="VF720_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7879080.1"
                     /translation="MNRPRAAIFDLDGTILDSLGLTFEAMREAVRPFVGRDLTDEEIY
                     ARFGPADHEIVAAFVKPEDANRAVRRLMAAYERGMERMAVYPGIMELLDSLAEHDVAL
                     ALCTGRGRPSTDLVLKALKLDAWFDVTVTGEEGGLPKPAPDGIRETLRLLGLEAGQAL
                     YTGDSVKDVDAGLAAGAATIAARWGGVESDEAGFGRAALQARTPNDILAWWESLPAAW
                     "
     gene            complement(1893..2657)
                     /locus_tag="VF720_06705"
     CDS             complement(1893..2657)
                     /locus_tag="VF720_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX7879081.1"
                     /translation="MIGVFAGRPAAVGALVLVLLVVAGCEDDPAGPTPPPLTGTGRLV
                     GSVSFDGFHGAPIPAATLRAEAEADGSVRGHVLSDGLTGAWSIDQLGAGTYRLVVEAV
                     CFGTITIENLEVDSLATVVPSIQLSVGTSPFQRIQLLGSFNGFDLDSAADMRKLAPCV
                     WVDTLDLTEVDVPIEFKFVTNGDFDTTPDYGQPGDQFDPASGPCRRGAAGASGNIRIQ
                     LAEPGRYFFEIDERFPRYIVARMGPIPTENGTAPLW"
     gene            complement(2699..2935)
                     /locus_tag="VF720_06710"
     CDS             complement(2699..2935)
                     /locus_tag="VF720_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879082.1"
                     /translation="MWFRTALLLFLLLLVIRFIRDVAARLKSPPSDGRTADTGFSEGS
                     KPSGAGKEGPSAANWPPGEITDVEYRESRTADRE"
     gene            complement(2941..3306)
                     /locus_tag="VF720_06715"
     CDS             complement(2941..3306)
                     /locus_tag="VF720_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879083.1"
                     /translation="MLVCPNCGNGFRDDEDFCLDCGLKVGTEEALEKHECVTGTRQMR
                     YRPVYKAPDEWMALTMRDLLSSAGLDARILTGSEPWSEGISRLAEGYWGKVVVLEVEV
                     DDARRLIEEYLKGLDRPVQ"
     gene            3450..3902
                     /locus_tag="VF720_06720"
     CDS             3450..3902
                     /locus_tag="VF720_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7879084.1"
                     /translation="MKNEVDWSRTCISCQYCCTYVSLAIDTPKTEADLDHIRWYLAHR
                     NIHIYVDHDDDWYFLVNNVCENLTAEGCGIYEQRFQICRDYSADTCEMTTGQPSEKVL
                     FRTVDDFDRWLERNREGVNERLAAARRNDPAIRPDARTMRGHLRTRGR"
     gene            complement(4092..5711)
                     /locus_tag="VF720_06725"
     CDS             complement(4092..5711)
                     /locus_tag="VF720_06725"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX7879085.1"
                     /translation="MFGRKRGAMIAVASLLATGVFSLTASAAENESSDGAAFLRGGVG
                     PRYLAMGKAGTAIANDVYAGYWNPAGLAYSCGWQVAGMYTGGLDFDRAHNFVGASYGK
                     AGNWGIGFGWTNAGTGDIQGRTNGGAPTEIFDYGENALALSFAKAFDRFSIGGTGKYV
                     VQDVGTSVGVTDDNATGFGLDIGAQFHAGQYLDLAVQAQDLWAEVGEDEGTNDVPTFL
                     KFGGAIYPTDGLTISSDVDLLQDDDGAFFRIGGEYAFDMGEDFDGAVRLGLDAGRFAG
                     GLGFGMDWVTFDYAYVVEPEDFQGSNHRLGLKLNFGCDEQPMMARSAGDRDHDGIPDD
                     TDRCPDQPEDFDGFQDTDGCPDPDNDGDGVVDAQDQCPGQAEDFDGFEDTDGCPDLDN
                     DKDGILDKDDKCPSAPETFNGYEDADGCPDDMPFCFPIAYINFKFNTAEMTHADYIPV
                     LEEVARIMKENSEIRVEIQGHTDSIGSDEYNQSLSERRSQAVKDYLIAKGIGADRMTT
                     RGFGESQPIDTNDTDLGRARNRRIEFKVIKQ"
     gene            complement(5830..>10453)
                     /locus_tag="VF720_06730"
     CDS             complement(5830..>10453)
                     /locus_tag="VF720_06730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879086.1"
                     /translation="YYYKLSTDLNVPSAWHFIDEEDNGFDESDENPDRNRPFSFDWNT
                     NAIADCDDVLIPGETYDIGFSITDRLCNELSVVAAHDAGYTSRLRVVDTIAPSATITE
                     LQREVGNSDIVEFPQSVRVRAINYLQATILNGHRDGESVSFYFTPKTGATAPTFSTAT
                     WTLADADIEEVGDQTWRMTNWDSSPLAQGEYWFVAIAEDCYTNRDDGSAPNSTQPPAI
                     VLIIDRTGPTFEGVNPSNAEVLVPEVTDCFSPNGARLIDLIVRNLDNDVDYTSFNDNN
                     IVFQYKKSETADREENWISITSQELRNGGTGNAAGQTPTFSAAINIRNLQGDGLYDFR
                     VCASDVAGNTTYYVFSSQAVVDNTKPVLEISYIEFTNRGDDHTPQPVDGGILPDISAG
                     ESIRLYVTAADDEPQIPNTGGTNHNEDLTGIQEIQFYLLSGPSGSGTYPQNLGTAGYD
                     ATTGQYFITWNTTALPDGDYTIQARGQDTVRNCTASQIVTGNVTNPAKPLAQVAAFNP
                     DLLSEITKGTNSRVYGLTFGEKLASTMFFQYRVVPAQGAPSQEWVNIGAATFTGETLV
                     SRELSLLPQSLWFSNMRISDLADNTNLDFRAVAVASSSVDSLGLGGTIATEPVNDTES
                     NYYGRTVSTGFATADGEAGDFSGLYDLQNTPILRMTKRSGPTGQAFLEYDQSLPGQPD
                     YISSVTAQGVDFTTNTLVTVNTTDPRVSPFVVIVGEDGTGDIDETILEVNRRIDNPRV
                     WTGDFRNGSTDFTDLDPTEGARIGVFATAHLQRPTDESAPPREDMAVKLWTIHQVTPA
                     AGSNGIAGVEGRNGLDANGCPIDGFAFEVPPGAFDYNMGMLLDTVNRPVTPTEQDLYI
                     SAFGPTYRVYFMNSDWEDCDGCANDDGWQSHGWMRYDDNDPAIRSEAEVTVRRWNGSE
                     WTASGISHIEVDTLNNCIHFTFDPNSTDQSGLNPIFSAVTTDRFSPVQVDAYPACRGY
                     TDQDPIWKTVLTNQGEAIDPGTIRIFVDDREVAAVRGYDAGANLNKTSASGGYSHWLL
                     YNGSWLDVEQISSDGRRWEVTFGWPCDNATELQGGQHTFEVWFSDYSRSTFYGVGQPY
                     IFEVDRTPPTIVLNGGFTGDPKQNTGAGYIGEETNAITVKLIDTESGVLFREDRIDDD
                     DYSHYWWDDVEDYCKIARYAVLYDAWFWENDIPIQFDSDNGFKYDLWVVDSENEDDQN
                     SVDEIEERTLIHTGTADELWPNVTTSEDGDTLIVPLYILGGGRIKDGDVLEVVLYSKR
                     YEIAPLTNLLNEYLDQISGQGGLYGDGWWVDFSSKRLVQYAASIQDCNENVGSRFVEQ
                     RFIVDKGAPGVEVTSPGVSCDGSTVTAVEPVADYVFHANFDDQGAGVDPSSVTVTVTG
                     PVADGDEDGVTITGLEVNEDGVTFTIPLDNLLVGQYTIRIKGEDNIGNKFDKTCVLFV
                     GGTSLALGNVVAFPNPFNPNATDLTLAFDSARAANVSVEIFDWNGDKVASINNTQVPA
                     GRSVIKWGGQSSDDKPLANGVYLARIDANDGTRTVTQVLKIAVWHD"
BASE COUNT         1691 a         3316 c         3516 g         1930 t
ORIGIN      
        1 agtgctgcgc gcgggcttcc acgtccgtcc tgggcatcca tgccaggttc agcagagtca
       61 tccgacatga actgcctcaa ttctacgaga acagattttc ctggcctccg ctggcgtttc
      121 ttgctctgtt tggctggatc cccaggtgct tgtacgccag ctccgtggcc atcctccccc
      181 gcggcgtccg ctccaggaat ccctcctgca cgaggaaggg ctcgtacacg tcttccatcg
      241 tgtcggcttc ctcgccgacg gccatggcca gcgtattgag gccgacgggg ccgccggcga
      301 acttctggat cagcgcctcg aggaggcggc gatccatgac gtcgagcccc ttcgtgtcga
      361 cctcgagcag gtcgaggctg gcccgggcga tgggcgcgtt gatcgtgccg tcccccttca
      421 cctcggcgta gtcgcgcacg cgtcgcagca ggcgattggc gatgcggggc gtgccgcggg
      481 cacggccggc gatttcggcg gcgccggcgt cgtcgatggg cacgtcgagg atgcgggcgg
      541 agcggcggac gatgacctgg agctcgtcgg cgtcgtagta gttgaggcgg taggtcatgc
      601 cgaagcggct gcgaagcggc gccgtgagga ggccggcccg cgtggtggcc cccaccagcg
      661 tgaatttcgg gagcgtgagg cggacggagc gggcgctcgg ccccttgtcg atcatgatgt
      721 cgagcgtgaa gtcttccatc gccgggtaga ggtactcctc caccgtatgg ttcagccggt
      781 ggatttcgtc gatgaacagg atgccccgct cggggatgtt ggtgagcacg ccggccaggt
      841 cgcccgggcg ctcgaggacg gggcccgacg tgaagaggat ttccacgccg agctcgcggg
      901 ccagcagcgt ggagagcgtg gtcttcccca ggcccggcgg accgtagaac agcacgtgct
      961 cgagggcttc gccccggcgc atggccgcct ggaggccgat tcccaggttt tccctgaggc
     1021 tccgctggcc gacgaagtcg tccatccgct ggggccgcag gttcgaatcg agctgggaat
     1081 cgtcgtcgag ccgttcggga tcggtgatgc cgcgcgtttc gtccatcatg tctttcgcgg
     1141 gggtcggggc ggtgtggatc cgtcgtgctg gccccgcaaa attaccatgc cgccgggagc
     1201 gattcccacc aggcgaggat gtcgttcggt gtgcgggcct ggagcgccgc ccggccgaat
     1261 ccggcttcgt ccgattccac gccgccccac cgcgccgcga tcgtggccgc tccggcagcc
     1321 aggccggcat ccacgtcctt gacggaatcg ccggtgtaga gcgcctggcc ggcctcgagg
     1381 ccgaggaggc ggagcgtctc ccgaatgccg tcgggggcgg gcttgggaag gcccccttcc
     1441 tcgccggtga ccgtgacgtc gaaccaggcg tcgagcttga gcgccttcag gacgaggtcg
     1501 gtgctcggtc ggccgcgccc ggtgcagagg gccagcgcca cgtcgtgctc ggccagggag
     1561 tcgagcagct ccatgatgcc cggatacacc gccatgcgct ccatcccgcg ttcgtaggcg
     1621 gccatcaggc ggcgtacggc ccggttggcg tcttcgggct tcacgaaggc cgccacgatc
     1681 tcgtggtcgg cggggccgaa acgggcgtag atctcctcgt cggttaggtc gcgcccgacg
     1741 aaggggcgga ccgcctcgcg catcgcctcg aaggtgaggc cgaggctgtc gaggatcgtg
     1801 ccatccaggt cgaagatggc ggcgcggggc cggttcatgc gattgatggt tctccggggg
     1861 gcggggcgcg gcgtggggcg tgagcgatca ccttaccaga gtggcgcggt gccgttctcc
     1921 gtcgggatcg gccccatccg ggccacgatg tagcgcggga agcgctcatc gatctcgaag
     1981 aaataacggc cgggctcggc gagctggatg cggatgtttc cggaggcacc cgccgcgccc
     2041 cgccggcaag gccccgaggc cggatcgaac tggtcgccgg gctgaccgta atcgggcgtg
     2101 gtgtcgaaat caccgttggt aacgaacttg aactcgatcg ggacatcgac ctcggtcagg
     2161 tcgagcgtgt ccacccagac gcacggggcc agctttcgca tgtcggccgc ggaatccagg
     2221 tcgaagccgt tgaagctccc gaggagctgg atgcgctgga acgggctcgt gccgaccgaa
     2281 agctggatgg acggcacgac cgtggcgagc gagtcgacct ccaggttctc gatggtgatg
     2341 gtgccgaagc agacggcctc caccaccagc ctgtaggtgc cggcgcccag ctggtcgatc
     2401 gaccaggccc cggtaaggcc atcggagagc acgtggccgc ggacagaccc atccgcttcg
     2461 gcttcggcgc ggagagttgc ggccgggatc ggagcgccgt gaaatccatc gaacgacacc
     2521 gagccgacca ggcgcccggt gccggtcagg ggcggtggag tcggaccggc ggggtcgtcc
     2581 tcgcacccgg cgaccacgag gaggaccagt accagggccc ccaccgcggc agggcggccg
     2641 gcgaacacac cgatcatcga atgcggactc ctggtttacg gaaggacatg tggcggggtc
     2701 actcccggtc cgcggtgcgc gattcccggt actcgacgtc ggtgatctcg ccgggaggcc
     2761 aatttgccgc ggaggggcct tcttttccgg cgccggatgg tttgctcccc tcgctgaatc
     2821 cggtgtcggc ggtccgcccg tccgagggtg gcgacttcag gcgcgccgcc acgtcgcgaa
     2881 taaatcgaat gacaaggagg agcaggaaga ggaggagggc ggtgcgaaac cacatgggag
     2941 ttactggacg gggcgatcca ggcccttcag gtattcctcg atgaggcgcc gcgcatcgtc
     3001 gacctcgact tcgaggacga ccaccttccc ccagtaacct tcggccagcc ggctgatccc
     3061 ttcggaccag ggctcgctgc cggtcaggat gcgggcatcg agaccggcgg aggagaggag
     3121 gtcgcgcatg gtgagcgcca tccattcatc aggcgccttg tagaccgggc ggtagcgcat
     3181 ctggcgtgtg ccggtgacgc attcgtgctt ttccagcgcc tcttcggtgc cgaccttgag
     3241 gccgcagtcc aggcagaaat cctcgtcgtc acggaagccg tttccacagt tcgggcagac
     3301 gagcattggg tgtcattcct cgcttccggc ggggtgggcc ccgatctccg accaccgccc
     3361 gccgacaaaa tggtagcaaa cgccttccgc gccgggcaag gcacgcacgc gcgttgaccc
     3421 accgccggcc acggtgctag cgttcgtgca tgaagaacga ggtcgactgg tcccgcacct
     3481 gtatttcctg ccagtactgc tgcacctacg tgagccttgc catcgacacg ccaaagaccg
     3541 aggccgacct cgaccacatc cgctggtacc tcgcgcatcg caacattcat atctacgtcg
     3601 atcacgatga tgactggtac ttcctcgtca acaacgtctg cgagaacctc acggcggaag
     3661 ggtgcggcat ttacgagcag cgcttccaga tctgtcgcga ctactccgcc gacacctgcg
     3721 agatgacaac cggccagccg tccgagaagg tgctcttccg gaccgtcgac gacttcgacc
     3781 gctggctcga acgcaatcgc gaaggcgtga atgagcgcct ggccgcggcc cgacggaatg
     3841 acccggccat ccggcccgat gcccgcacga tgcgcgggca cctccgtacg cgcggccgct
     3901 aggccgcccc ctacccacac ccacggccgg gcaagctccc cccgccccga tcgtcgcgcg
     3961 gccgtcgcct gtcccgaacc ttcctcggcc ccgccagccg cacaaaaaga aacgccgccg
     4021 gctcctgtga gccggcggcg cgtccttttg gtcggaagct tacgggaaac tgtggtggtg
     4081 ccgaagcacc gttactgctt gattaccttg aactcgatcc ggcggttacg ggcccgtcca
     4141 agatcggtgt cgttggtgtc gatgggctgg ctctcgccga agccgcgggt cgtcatgcga
     4201 tccgcgccga tgcccttcgc gatcaggtaa tccttgacgg cctgcgaacg acgctcggac
     4261 agcgactggt tgtactcgtc gctgccgatc gagtccgtgt ggccctggat ctcgacacgg
     4321 atttccgaat tctccttcat gatacgggcc acttcctcga gaaccgggat gtagtccgca
     4381 tgggtcattt cggccgtgtt gaacttgaag ttgatgtagg cgatcgggaa gcagaacggc
     4441 atgtcatccg ggcagccgtc ggcgtcctcg taaccattga aggtttcggg cgcgctcggg
     4501 cacttgtcgt ccttgtccag gatgccgtcc ttgtcgttgt cgaggtcggg gcagccatcg
     4561 gtgtcctcga acccgtcgaa gtcttccgcc tggcccgggc actgatcctg ggcatcgacc
     4621 acgccgtcac cgtcgttatc cggatcgggg cagccgtcgg tgtcctggaa gccgtcgaag
     4681 tcttccggct ggtccgggca acgatcggtg tcgtccggaa tgccgtcgtg gtcgcggtcg
     4741 ccggcgctgc gggccatcat cggctgctca tcgcacccga agttgagctt gaggccaagg
     4801 cgatggttcg agccctggaa gtcctcgggc tcgacgacgt aggcgtaatc gaacgtgacc
     4861 cagtccatcc cgaagccgag gccgccggcg aaccgcccgg catcaagacc cagtcggacc
     4921 gcgccgtcga aatcctcgcc catgtcgaac gcgtactcgc cgccgatgcg gaagaacgcg
     4981 ccgtcgtcgt cctggagcag gtcgacgtcg gacgagatgg tcaggccgtc ggtcgggtag
     5041 atggcgccgc cgaacttgag gaaggtcggg acgtcattgg ttccctcgtc ttcgcccacc
     5101 tcggcccaga ggtcctgggc ctggacggcg aggtcgaggt actgcccggc gtggaactgc
     5161 gcgccgatgt cgaggccgaa gcccgtggcg ttgtcatcgg tgacgccgac actcgtgccg
     5221 acatcctgga cgacgtactt gcccgtaccg ccgatcgaga aacgatcgaa ggccttggcg
     5281 aacgacaggg ccagcgcatt ctcgccgtaa tcgaagatct cggtgggcgc gccaccgttg
     5341 gtgcggccct ggatgtcgcc cgttccggcg ttcgtccagc cgaaaccgat gccccaattg
     5401 ccggccttgc cgtagctggc gccgacgaag ttgtgggcac gatcgaaatc caggccgccg
     5461 gtgtacatac ccgcaacctg ccagccgcac gagtaggcca ggccggccgg gttccagtaa
     5521 ccggcataga catcatttgc aatggccgtt cccgccttgc ccatcgccag ataacgaggg
     5581 ccgacgccgc cgcgaaggaa cgcggccccg tccgaactct cgttctccgc tgcggaggcg
     5641 gtgagcgaga acacgccggt agccagcagg ctggcaaccg cgatcatcgc tcctctcttc
     5701 cgaccaaaca tcagcaaacc tccttttcca cagtttcccc gcttcacttg cttcccgttg
     5761 attccgtagt tcgtttccga cttgggtgaa tccggggaga gggcccgcgc cccctccccg
     5821 gacttgtgat tagtcatgcc agaccgcgat cttgaggacc tgggtcacgg tgcgggtacc
     5881 gtcgttcgcg tcgatgcgcg cgaggtagac accgttcgcc agcggcttgt cgtcagacga
     5941 ctgaccgccc cacttgatca ccgaccggcc cgccggcacc tgggtgttgt tgatgctggc
     6001 caccttgtca ccgttccagt cgaagatctc gaccgagacg ttggcggcgc gggcggagtc
     6061 gaacgccagg gtcaggtccg tggcgttcgg gttgaacggg ttcgggaagg ccaccacgtt
     6121 gccgagcgcc agcgaggtgc cgccgacgaa caggacgcag gtcttgtcga acttgttacc
     6181 gatgttgtcc tcgcccttga tccggatcgt gtactgaccc acgagcaggt tgtcgagcgg
     6241 gatcgtgaac gtcacaccgt cctcattcac ttcgaggccg gtgatcgtca cgccatcttc
     6301 gtcgccgtcc gcaaccggac cggtcaccgt caccgtcacc gaggacggat caacaccggc
     6361 accctggtcg tcgaagttgg cgtggaagac gtagtcggca accggctcga cggcagtcac
     6421 ggtcgaaccg tcgcagctga cacccggcga ggtcacttcg accccaggag cgcccttgtc
     6481 gacgatgaac cgctgctcga cgaaccgcga accgacgttc tcgttgcagt cctggatcga
     6541 ggccgcgtac tgcacgaggc gcttgctgga gaagtcaacc caccagccgt caccgtacag
     6601 accgccctga cccgagatct ggtcgaggta ctcattcagg agattggtca gcggagcgat
     6661 ttcgtaccgc ttgctgtaga gcaccacttc gaggacgtcg ccgtccttga tgcggccgcc
     6721 gcccaggatg tagagcggga cgatgagggt atcgccgtct tcgctcgtgg tgacattcgg
     6781 ccagagctcg tcggccgtgc cggtgtggat cagagtccgc tcctcgatct catcgaccga
     6841 gttctggtcg tcctcgttct cggaatccac gacccacagg tcgtacttga agccgttgtc
     6901 cgaatcgaac tggatgggga tgtcgttctc ccagaaccag gcgtcgtaca ggaccgcgta
     6961 gcgcgcgatc ttgcagtaat cctcgacgtc atcccaccag tagtggctgt agtcgtcgtc
     7021 gtcgatccgg tcttcacgga agaggacacc cgactccgtg tcgatgagct tcacggtgat
     7081 cgcgttggtc tcctcgccga tgtagccggc gccggtgttc tgcttcgggt cgcccgtgaa
     7141 gccgccgttc agaacgatcg tcggcggggt gcggtcaacc tcgaagatgt agggctggcc
     7201 cacgccgtag aaggtgctcc gcgagtaatc ggagaaccac acctcgaacg tgtgctgccc
     7261 accctggagc tcggtggcgt tgtcgcaggg ccagccgaag gtcacttccc aacggcgacc
     7321 atcgctggag atctgctcga cgtcgagcca ggaaccgttg tagaggagcc agtggctgta
     7381 accgccgctg gcggacgtct tgttcaggtt ggcgcctgcg tcgtagccac ggacagccgc
     7441 aacttcacgg tcgtccacga agatccggat cgtgccggga tcgatcgcct caccctggtt
     7501 ggtgaggacg gtcttccaga tcgggtcctg atccgtgtag ccgcggcacg ccgggtaggc
     7561 gtcgacctgg accgggctga accggtcggt cgtgaccgcg ctgaagatcg gattgagacc
     7621 gctctggtcg gtcgagttcg gatcgaaggt gaagtggatg cagttgttca gcgtgtccac
     7681 ttcgatgtgg ctgatgccgg aggccgtcca ctcggagccg ttccagcgac ggaccgtgac
     7741 ttccgcttcg gagcggatcg ccgggtcgtt gtcgtcgtaa cgcatccagc cgtgggactg
     7801 ccagccgtca tcgttggcgc agccgtcgca gtcttcccaa tcgctgttca tgaagtagac
     7861 gcgataggtc ggaccgaagg ccgagatgta gagatcctgc tcggtcggcg tgaccggacg
     7921 gttcaccgtg tcgagcagca tgcccatgtt gtagtcgaac gcacccggcg ggacttcgaa
     7981 ggcgaagccg tcgatcgggc aaccgttcgc gtcgaggcca ttccgacctt cgacacccgc
     8041 gatgccattg gagccggccg ccggagtcac ctggtggatg gtccagagct tcaccgccat
     8101 gtcttcacgg ggcggggccg attcgtccgt cggccgctgc aggtgcgcag tggcaaacac
     8161 gccgatccgg gcgccttcgg tcggatcaag gtcggtgaag tccgtcgagc cgttccggaa
     8221 atcgccggtc cagacccgag gattgtcgat acgacggttg acctcgagga tcgtctcgtc
     8281 gatgtcaccc gttccgtcct cgcccacgat caccacgaaa ggcgacaccc tgggatcggt
     8341 cgtgttgacc gtcacgaggg tgttggtggt gaagtcgaca ccctgcgcgg tgaccgagct
     8401 gatgtagtcc ggctggccgg ggagcgactg gtcgtactcg aggaaagcct ggccggtcgg
     8461 gccgctccgc ttggtcatcc ggagaatcgg ggtgttctgc aggtcgtaca gacccgagaa
     8521 gtcgcccgcc tcaccgtccg ccgtggcgaa gccggtcgag accgtccgtc cgtagtagtt
     8581 gctctcggtg tcgttgaccg gctcggtggc gatggtgccg cccagaccga gcgaatcgac
     8641 cgacgacgag gcgaccgcca cggcgcggaa gtccaggttc gtgttgtcgg caaggtcgct
     8701 gatgcgcatg ttgctgaacc acagcgactg cgggagcagc gagagctcgc ggctgaccag
     8761 cgtttcgccc gtgaaggtgg cggccccgat gttgacccat tcctgcgacg gcgcaccctg
     8821 tgcggggacc acgcggtact ggaagaacat ggtgctggcc agcttctcgc cgaacgtcag
     8881 gccgtacacg cggctgttcg tgcccttggt gatctcggag agcaggtcgg gattgaaggc
     8941 ggcaacctgg gcaagcggct tggccgggtt cgtcacgtta cccgtgacga tctggctggc
     9001 cgtgcagttg cgcaccgtgt cctgaccgcg cgcctggatc gtgtagtcac catccggcag
     9061 ggccgtggtg ttccaggtga tgaagtactg gccggtggtg gcgtcgtagc cggcggtacc
     9121 caggttctgc gggtacgtgc cgctgccgct cggtccggac agcaggtaga actggatctc
     9181 ctggataccg gtcaggtctt cgttgtggtt cgtgccaccg gtattcggga tctgcggttc
     9241 gtcgtccgcg gccgtgacgt acagacggat cgactcgccc gccgagatgt ccggcaggat
     9301 gccgccgtcc accggctgag gcgtgtgatc gtcgccacgg ttcgtgaact cgatgtagct
     9361 gatttccagg accggcttgg tgttgtcgac cacggcctgg ctggagaaga cgtagtaggt
     9421 ggtgttgccc gccacgtcgc tcgcacagac acggaagtcg tagagaccgt caccctgcag
     9481 gttccggata ttgatggccg ccgagaaggt cggggtctgg ccggccgcat tgccggtgcc
     9541 accgttgcgg agttcctggc tcgtgatgct gatccagttc tcctcgcggt ccgccgtctc
     9601 ggacttcttg tactggaaga cgatgttgtt gtcgttgaag ctcgtgtagt cgacgtcgtt
     9661 gtccaggttg cggacgatga ggtcgatcag acgcgcgccg ttcgggctga agcagtccgt
     9721 cacctcgggc accaggacct cggcgttgct cgggttcaca ccctcgaacg tcgggccggt
     9781 gcggtcgatg atcaggacga tcgccggcgg ctgggtggag ttcggggccg aaccgtcatc
     9841 gcggttggtg tagcagtctt ccgcgatggc gacgaaccag tactcaccct gggccagcgg
     9901 gctcgaatcc cagttggtca tgcgccaggt ctggtcaccg acctcttcaa tgtcggcgtc
     9961 cgcaagcgtc cacgtggcgg tgctgaaggt cggcgcggta gcaccggtct tcggcgtgaa
    10021 gtagaacgac acgctctcgc cgtcacgatg gccgttgagg atggtggcct ggaggtagtt
    10081 gatcgcccgg acgcggacgc tctgcgggaa ctcgacgatg tccgagttgc cgacttcgcg
    10141 ctgcagctcc gtgatggtgg cgctcggcgc aatggtgtcg acgacacgga ggcgcgaggt
    10201 gtaacccgcg tcatgcgccg cgacgacact cagctcgttg cagaggcgat cggtgatcga
    10261 gaagccgatg tcgtacgtct cgcccgggat caggacgtca tcgcagtcgg cgatggcgtt
    10321 cgtgttccag tcgaacgaga acggacggtt gcgatcggga ttctcgtcgc tctcgtcgaa
    10381 cccgttgtcc tcttcgtcga tgaagtgcca ggccgaagga acgttcaggt ccgtgctcag
    10441 cttgtagtag tag
//
LOCUS       DASVOD010000300         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_490606, whole genome shotgun sequence.
ACCESSION   DASVOD010000300 DASVOD010000000
VERSION     DASVOD010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490606"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF720_06735"
     CDS             complement(<1..216)
                     /locus_tag="VF720_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879087.1"
                     /translation="MTHLAIRDAVLDDAEALARLVTDLGYPTSVEQMRMRLGRIRSDD
                     DYRTLVAIDLDDIVGFIGLRIGLNYASD"
     gene            complement(239..769)
                     /locus_tag="VF720_06740"
     CDS             complement(239..769)
                     /locus_tag="VF720_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7879088.1"
                     /translation="MSTLAAPGAGEFAPFYAGYIATAGGDPLGLMERQVARFRRLGET
                     LTEAQAAHRYAPGKWSVKEVLGHLIDGERVFCYRALRIARGDATPLAGFDENVYVAAA
                     GSDRRTAADLAGELVALRMANLAFFHSLDDVAFGRIGVANDVPVSVRALAFIMAGHTE
                     HHLGVIGERYGIATAG"
     gene            complement(805..1506)
                     /locus_tag="VF720_06745"
     CDS             complement(805..1506)
                     /locus_tag="VF720_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879089.1"
                     /translation="MQRYHAIDYAELDASLAAGREVSVLRTSRRGGWWWALLVLGITA
                     CERSSPGDAQGPADPTVLPDPPNPVAPPDPIDPPSLRHPPSSLPARLLVTRQWSGSAG
                     GHTDDGDGYRGLSYRSTFVLENDDSLRVFWEAAHPGSGPPPPRPMLDFNREVMLVTTH
                     GWNTGCVAFSIDSLDVSPDRTVAFITSTRHSGRACILCEYSFSRIWTAAAIPKLPGPV
                     EPRFTYIGVSCGGGP"
     gene            complement(1520..2668)
                     /locus_tag="VF720_06750"
     CDS             complement(1520..2668)
                     /locus_tag="VF720_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional activator/DNA
                     repair protein Ada/methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX7879090.1"
                     /translation="MTARRKLPRMTNLTPVRPTRTTLPARPVMERAVIDRDDSFDGLF
                     TVAVTSTNIFCRPSCPARKPLRKNMDFYPTPKEALAAGFRPCKRCRPMAVNGKPPAWV
                     SGLLDRIELDPERRPHDADLRAWGVDPVKARRWFLKNHAMTFHAYARARRLGKAFQKI
                     RKGAPLDDTGMDVGYESTSGFRDAFGQIFGTPPGKARTGDCIVTAMIPTPLGPLVAGA
                     TSKGLCLLEFTDRRMLEAQVTTLRRHFKAPIVPGSNRHLVQARRELKEYFAGRRKKFE
                     VAVDAPGTEFQLQVWKELRRIPSGATQSYAEIARRVGRPGAVRAVGTANGMNRIAIII
                     PCHRVLNTGGKLGGYGGGLWRKQALLELEGVALLSLGLERAGSSSPRP"
     gene            complement(2665..>3060)
                     /locus_tag="VF720_06755"
     CDS             complement(2665..>3060)
                     /locus_tag="VF720_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018508065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX7879091.1"
                     /translation="GAFARGLASGRRAALALHAHLAAHPGFTPLLVPELDIVVWAVRA
                     EQASVASGLSRQLFEAAARHDLHLAVATLPRRLAEKARPVSEWDEEMLVCLRACVMKP
                     EHEAWLPEIVVRLDRAAAECGVQTAVERP"
BASE COUNT          500 a         1044 c         1041 g          475 t
ORIGIN      
        1 gtcactggca tagttcaggc cgatgcggag gccgatgaag ccgacgatgt catcgaggtc
       61 gatcgccacc agcgtccggt agtcgtcgtc ggaacggatg cggccaagcc gcatcctcat
      121 ctgttcgacg gacgtcggat acccgaggtc ggtgacgagg cgcgccagcg cctccgcatc
      181 gtcgagcacg gcgtcccgga tcgcgaggtg cgtcatttcc tgttcgtcgc gcggtgggtc
      241 agccggccgt cgcgatcccg tagcgctcgc cgatcacgcc caggtgatgt tccgtgtgcc
      301 ccgccatgat gaaggccagc gcgcgcacac tgaccggcac gtcgttggcc acgccgatcc
      361 gcccgaacgc gacatcatcc agcgagtgga agaacgccag gttggccatc cggagcgcca
      421 cgagttcacc cgccaggtcc gcggccgtgc gccgatccga accggccgcc gccacgtaga
      481 cgttctcgtc gaaccccgcc agcggcgtgg catcgccccg cgcgatccgc agcgcgcggt
      541 aacagaacac gcgttcgccg tcgatgaggt gaccgagcac ctccttcacg ctccacttcc
      601 ccggcgcgta gcggtgcgcg gcctgggctt cggtcagcgt ctcgccgagc cggcgaaagc
      661 gggccacctg ccgctccatg aggccgaggg ggtcgccgcc cgccgttgcg atgtatcccg
      721 cgtagaaggg ggcgaattca ccggcgccgg gtgctgcgag tgtcgacatt gatgtcctcc
      781 gttggtttta tgttcagccc ggcatcaggg gccacccccg cacgagactc cgatgtaagt
      841 gaagcggggt tccacggggc ccggtaattt gggaatggcc gccgctgtcc agatgcgcga
      901 aaaggaatat tcgcacagga tgcaggcgcg gcccgagtgc cgggtgctcg tgatgaaggc
      961 cacggtacgg tcgggagata catcgaggct gtcgatcgaa aacgcgacgc accccgtgtt
     1021 ccaaccgtgc gtggtgacga gcatgacttc ccggttgaag tccagcatgg ggcggggggg
     1081 tgggggacca gatccgggat gcgcggcctc ccagaacaca cgcaacgagt cgtcgttttc
     1141 cagcacgaag gtggacctgt agctcaagcc ccgatagccg tctccatcgt cggtgtgccc
     1201 cccggcactg ccgctccact gtcgcgtaac caggagacgt gccggtaagc ttgaaggtgg
     1261 atggcgcagg ctcggcgggt cgatcgggtc aggtggcgcc accggattgg gagggtccgg
     1321 caggaccgtc gggtctgccg gaccctgcgc gtctccgggt gacgatcgtt cgcacgccgt
     1381 gatgccgagc acgaggagcg cccaccacca accgcctcgc cttgaggtcc gaagaacgct
     1441 cacttctcgc ccggccgcga ggctggcatc gagctcggcg taatcaatgg cgtgatatcg
     1501 ctgcattcgg actccgccat cagggcctgg gggaagaaga acccgcgcgc tccaacccga
     1561 gcgacaacag cgccacccct tccaactcca gcagcgcctg cttccgccag agcccgccgc
     1621 cgtaaccccc gagcttgccg ccggtgttca acacgcgatg gcagggaatg atgatggcga
     1681 tgcggttcat cccgttggcg gtgccgaccg cccgcaccgc cccgggtcga cccacgcgcc
     1741 gggcgatttc ggcgtagctc tgcgtggcgc ccgaagggat gcgccgcagc tccttccaca
     1801 cctgcaactg aaactcggtg ccgggggcat cgaccgccac ctcgaacttc tttcgacggc
     1861 cggcgaagta ctctttcagc tcacgccgcg cctgcacaag atgacggttg ctccccggaa
     1921 cgatgggcgc cttgaaatga cggcgcagcg tggtcacctg cgcctcgagc atgcggcgat
     1981 cggtgaattc gaggaggcag agccccttcg aggtggcgcc ggccacgagt ggcccgaggg
     2041 gtgtcgggat catggcggtc acgatgcagt cgccggtgcg ggccttgccg ggcggcgtgc
     2101 cgaagatctg gccgaaggcg tcgcgaaagc cgctggtcga ttcgtagccg acgtccatgc
     2161 cggtgtcgtc gaggggcgca cccttccgga tcttctggaa ggcttttccg agccgccgcg
     2221 cacgggcata cgcatggaac gtcatcgcgt gattcttgag gaaccagcgc cgtgccttca
     2281 cgggatcgac gccccacgcg cgcaggtcag catcgtgcgg gcgccgctcg ggatcgagtt
     2341 cgatccgatc gagcagcccg ctcacccatg ccggcggctt gccgtttacc gccatcggac
     2401 gacaccgctt gcaggggcga aagcccgccg ccaacgcctc cttcggcgtc gggtagaagt
     2461 ccatgttctt ccggagcggc tttcgcgccg ggcacgacgg gcggcagaag atgttggtgg
     2521 aggtgacggc cacggtgaag aggccatcga acgagtcgtc gcgatcgatc acggcgcgct
     2581 ccatgacggg gcgggccgga agcgtggtcc gcgtggggcg gacaggagtg aggttcgtca
     2641 tgcggggaag cttgcgccgg gcggtcatgg gcgctccacc gcagtttgaa caccgcattc
     2701 ggcggccgcc cgatcgagcc ggaccacgat ctccggcagc cacgcctcgt gctccggctt
     2761 catcacgcag gcgcgcaggc agacgagcat ttcctcgtcc cactcggaca ccggccgggc
     2821 cttttccgcc agccggcgcg gcaacgtggc caccgccagg tgcaggtcgt gccgcgcggc
     2881 cgcctcgaag agctgccgcg acagtcccga agccaccgac gcttgttccg cccgcaccgc
     2941 ccacaccacg atgtcgagct ccggcacgag caagggcgtg aagccgggat gggccgccag
     3001 gtgcgcgtgg agcgccagcg cggcccgccg tcccgacgcg agtccccgcg cgaacgcgcc
//
LOCUS       DASVOD010000301         4669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_177819, whole genome shotgun sequence.
ACCESSION   DASVOD010000301 DASVOD010000000
VERSION     DASVOD010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4669
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177819"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="VF720_06760"
     CDS             <1..1530
                     /locus_tag="VF720_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879092.1"
                     /translation="HWPTSTKNEMAQRFFDQGIALTYGFNHAEAERSFLEATRLEPDF
                     ALAWWAAALVLGPNINVPMDTSVVAEARRLTDEAMSRRPKASPVEKALIEALDRRYSA
                     NPPADRAPLDVAYANAMRKVAKDFPENLDVVAMAAEAIMDLTPWNYWTMDGKPNVNTN
                     EIIGMLEQVLAADPSHPGAIHFYIHLTEASEHPEKAEPYADKLPALVPGSGHLVHMPS
                     HLYIRTGRYQDAIELNDLAAKADDSYLAQCHAQGVYEIGYVPHNHHMKFAAACLSGQS
                     AVARAAAAGVDSRTIHDMMRAPGLGVLQQYAYVGMYGLVRFGNWSDILALPAPPADLK
                     YPTGVWHFARGMAFAGTDKLEDAQKELDELAIFASMDTLEHVTIFETNTTKHLLEIAR
                     ETLAGEIAARRGEWTKAIEHFQAGVASEDKLRYNEPTDWNPAVRHFLGWAYLESKQPE
                     KAEKVYRDDLHRYPGNGWALLGLSQSLAAQGKAKEAAAAKDDFLKSWSRADVAISASR
                     L"
     gene            complement(1552..2700)
                     /locus_tag="VF720_06765"
     CDS             complement(1552..2700)
                     /locus_tag="VF720_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7879093.1"
                     /translation="MNFELDDDQRLLRDTVREFLEAEVAPHVEHFEKAGTLPRDILTR
                     LWNELGLGGMVIPEEYGGIGLDAVAYCLVIEEMARVWAALSITVAVHNSVGAGPIVQF
                     GNEAQKKKYLPKLATEWIGAFSLSEPVSGSDAAALRTTAVKDGNEYVLNGEKNWVTTG
                     GFADTYVVMARTAPDKQKGISAFIVEKGTPGLTLTKKEEKMGLLASETRSLVFQDCRV
                     PAENLLAEEGMGLKIALTLLNSGRFGVAAQSIGIARAALEASIRYARERETFGKPIGL
                     HQAVGFMLADMHTKLEASRALAWRAAWLKTAGKPFAREASMAKLFASEASTFITHRAI
                     QIHGGYGYVRDYPVERYYRDARVTEIYEGTSEIQRMVIATGLLKDAVG"
     gene            complement(2697..3839)
                     /locus_tag="VF720_06770"
     CDS             complement(2697..3839)
                     /locus_tag="VF720_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7879094.1"
                     /translation="MDFSLTDDERLIQDTARQIAREVIAPRAAETDATATFPHDNVTK
                     LAELGFMGMMVSEEFGGAALSTMAYALAIEEISKACASTGVIMSVNNSLVCHPIEKFG
                     TDAQKKKYLPKLAAGEWLGAYCLSEPDTGSDAARQKTVAIRKGDRYVLNGTKNFITNG
                     QVADVYIVYCMTDVAAGHRGISSVIVEATTPGLERGAKERTMGIRGSSTCQIILTDCE
                     IPAENLLGAEGEGFKVALNTLDGGRIGIASQALGIGAACLADSTRYANERHAFGEAIG
                     QFQAIQWMIADMATELDAARLLTWQAAAAKDAGRKYTMEASVAKAFASRMAVMNADRA
                     VQVHGGYGYLQDYAVERYYRDAKVTEIYEGTTEIQKHIIATGLLKL"
     gene            4172..4411
                     /locus_tag="VF720_06775"
     CDS             4172..4411
                     /locus_tag="VF720_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879095.1"
                     /translation="MHADTSGRIYVVGWDGTALCLDPNGIPLLYWTVPGAWARITTDA
                     AGLIYLAGTSADVRVYGSPTPVQPATWSAVKALFD"
BASE COUNT          784 a         1569 c         1513 g          803 t
ORIGIN      
        1 cactggccga cctccaccaa gaacgagatg gcccagcgtt tctttgacca ggggattgcc
       61 ctgacctacg gtttcaacca cgccgaggcc gagcggtcct tcctcgaagc cacgcgactc
      121 gagcccgact ttgcgctggc ctggtgggcc gccgcgctgg tcctgggtcc gaacatcaac
      181 gtgccgatgg acacgtcggt cgtggccgag gcgcgtcgcc tgacggatga ggcgatgtcg
      241 cggcggccga aagcctcgcc ggtggaaaag gccctcatcg aggcgctgga ccggcgctac
      301 agcgccaacc cgccggccga ccgcgcgccg ctcgacgtgg cgtacgccaa cgccatgcgg
      361 aaggtggcga aggacttccc cgaaaacctc gacgtggtgg cgatggccgc cgaggccatc
      421 atggacctga cgccctggaa ctactggacc atggacggca agcccaacgt gaacacgaac
      481 gagatcatcg ggatgctcga gcaggtgctg gcggctgatc ccagccaccc gggcgccatc
      541 cacttctata tccacctgac ggaggcctcg gaacatcccg agaaggccga accgtacgcc
      601 gacaagctcc cggcgctggt gccgggctcc ggccacctcg tgcacatgcc gagccacctc
      661 tatatccgga ccgggcgcta ccaggatgcc atcgagctga acgacctggc ggccaaggcg
      721 gatgacagct atctcgcgca atgtcacgcc cagggcgttt acgagatcgg gtacgtgccg
      781 cacaaccatc acatgaagtt cgcggcggcc tgcctgtcgg gccagagcgc cgtggcgcgg
      841 gcggcggcgg ccggggtgga ttcgcgcacg attcacgaca tgatgcgcgc ccccgggctc
      901 ggcgtgctgc agcagtacgc ctatgtcggg atgtacggac tcgtccgttt cggcaactgg
      961 agcgacatcc tggcgctccc ggcacctccg gccgacctca agtaccccac cggcgtctgg
     1021 cacttcgccc gtggcatggc cttcgccgga accgacaagc tcgaggacgc gcaaaaggag
     1081 ttggacgagc tcgccatctt cgcatcaatg gacacgctcg agcacgtcac gatcttcgag
     1141 accaacacca ccaagcacct gctcgagatc gcccgcgaaa ccctggccgg cgaaatcgcc
     1201 gcccgccgcg gcgagtggac gaaggccatc gaacacttcc aggccggcgt ggccagcgag
     1261 gacaagctgc gctacaacga gcccaccgac tggaacccgg ccgtgcgcca tttcctcggc
     1321 tgggcttacc tcgaatcgaa gcagcccgag aaggccgaaa aggtgtaccg cgacgacctg
     1381 caccgctatc ccggcaatgg ctgggcgctg ctcgggttgt cgcagagcct cgcggcccag
     1441 ggaaaggcga aggaggccgc ggcggccaag gatgacttcc tcaagtcctg gagccgggcg
     1501 gacgtggcaa tttcggcgtc gcggttgtag cccgttgcga caggcgacgc gtcagccgac
     1561 cgcgtccttg agcagcccgg tcgcaatcac catccgctgg atttccgagg tgccttcgta
     1621 gatctcggtg acgcgcgcat cccggtagta ccgctccacc ggatagtcgc gcacgtaacc
     1681 gtaaccgccg tggatctgga tggcgcggtg cgtgatgaag gtggaggctt cgctggcgaa
     1741 gagcttggcc atcgaggctt cgcgcgcgaa gggcttgccg gcggtcttga gccaggcggc
     1801 gcgccacgca agggcgcgtg aggcttcgag tttggtgtgc atgtcggcca gcatgaaccc
     1861 cacggcctgg tgcaggccga tcggcttgcc gaatgtctcg cgctccctgg cgtacctgat
     1921 cgaggcctcg agcgcggcgc gggcgatgcc gatcgactgg gccgccacgc cgaagcgccc
     1981 gctgttgagg agcgtgagcg cgatcttcag gcccatcccc tcttcggcca ggaggttctc
     2041 ggccggcacg cggcaatcct ggaagacgag gctgcgcgtc tcggaggcga ggagccccat
     2101 cttctcttcc ttcttggtca gcgtgaggcc gggcgttccc ttctcgacga tgaaggcgct
     2161 gatccccttc tgcttgtcgg gggcggtacg cgccatgacc acgtacgtgt cggcgaagcc
     2221 gccggtcgtc acccagttct tctcgccgtt cagcacgtat tcgttgccgt ccttcaccgc
     2281 ggtcgtgcgg agcgccgcgg cgtcggatcc ggacacgggt tccgacaggc tgaaggcgcc
     2341 gatccactcg gtggccagct tcggcaggta cttcttcttc tgcgcctcgt tgccgaactg
     2401 cacgatcggg ccggcgccca ccgagttgtg caccgccacc gtgatcgaca aggccgccca
     2461 cacgcgggcc atctcctcga tcaccaggca gtaggccacc gcgtcgagtc cgatgccgcc
     2521 gtactcctcg ggaatgacca tgccgccgag gcccaattcg ttccaaagtc ttgtgaggat
     2581 gtcacgcggc agcgtgcccg ccttctcgaa gtgttcgacg tgcggcgcca cctcggcttc
     2641 gaggaattcg cgcaccgtgt cgcgcaggag gcgctggtcg tcgtcgagtt cgaagttcat
     2701 aacttgagga gtcccgtggc gatgatgtgc ttctggattt cggtggtccc ttcgtagatc
     2761 tcggtgacct tggcgtcgcg gtagtagcgt tcgaccgcgt agtcctggag gtatccgtag
     2821 ccgccgtgca cctgcaccgc gcggtcggca ttcatgaccg ccatgcggct ggcaaaggcc
     2881 ttggccaccg aggcctccat cgtgtacttg cgcccggcat ccttggccgc ggccgcctgc
     2941 caggtgagaa ggcgcgccgc atcgagctcg gtggccatgt cggcgatcat ccactggatg
     3001 gcctggaact gcccgatggc ctcgccgaag gcatgccgct cgttggcgta gcgcgtggag
     3061 tccgcgagac aggccgcgcc gatcccaagc gcctgcgagg cgatgccgat gcgcccgccg
     3121 tcgagcgtgt tgagcgccac cttgaagccc tcgccctccg cgccgagaag gttctccgcc
     3181 ggaatctcac agtcggtaag gatgatctgg caggtggagg agcctcggat ccccatcgtc
     3241 ctttccttgg cgccgcgctc gagccccggc gtggtggcct cgacaatgac ggaggaaatg
     3301 ccgcggtgcc ccgccgccac gtcggtcatg cagtagacga tgtagacgtc ggccacctgg
     3361 ccgttggtga tgaagttctt ggtgccgttg aggacgtagc gatcgccctt tcggatcgcg
     3421 acggtctttt gccgggccgc gtcggatccc gtgtccggtt cggacaggca gtacgccccg
     3481 agccactcgc cggccgccag cttcggcaga tacttcttct tctgcgcgtc ggtcccgaac
     3541 ttctcgatcg gatggcacac cagcgagttg ttcaccgaca tgatcacgcc ggtcgaggcg
     3601 catgccttcg agatctcctc gatggccagc gcgtaggcca tcgtgctgag cgccgctccc
     3661 ccgaactcct cggacaccat catccccatg aagccgagct cggccagctt cgtcacattg
     3721 tcgtgcggaa atgtggcggt ggcgtccgtc tcggcggccc gcggcgcgat gacctcccgg
     3781 gcgatctggc gggcggtgtc ctggatgagg cgttcgtcgt cggtgaggga gaagtccatg
     3841 gggctccggg tgggggccgc tcgcgacggc aagtcggcga aaaaagctac aacgtcaaac
     3901 ggtcaaatag gttagcacga tgggatgggg tcagacaacg agaaaaggcg ctcctggatg
     3961 ccggcactcg atctgtattg gatgcaggtc agagcagagc ccagtcgagt ccgcaggcaa
     4021 gatctctagg caatacagcc aactgttgtt gacaacagtc cgtcccggtc cgagagacga
     4081 gatcgagtga ccgtgccgtg cgctatccga aggaacatgg ctgcgcagca tcgacctctc
     4141 ggcggccccg cttcggtctc aaccggccgg ggtgcacgcc gacacctccg gacgcatcta
     4201 cgttgtcggc tgggacggga cggcgctgtg cctcgacccg aatgggattc cgttgctcta
     4261 ctggacggtg ccgggtgcgt gggcgcgcat caccaccgac gcggccggct tgatctacct
     4321 ggccggtacc agcgccgacg tgcgcgtgta tggctccccc acccccgtgc aacccgccac
     4381 ctggagcgca gtcaaggccc tgttcgactg acattggagg cccggctccg ccgggccgac
     4441 tccattcctc ttccgccttc ggcgggacat cggcgcggcg accgcttgat ggggctttcc
     4501 tccgctgata gcctttcccg cttcccttgc accaaccacc accggaaacc gacatgcgcg
     4561 cctccacccc caagcactgg gacgactact ggaagaagca ggacgacctg gacgacgcct
     4621 attccaacga ggacaggctc atcgacgggt tgctccagct catggatcc
//
LOCUS       DASVOD010000302         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_803083, whole genome shotgun sequence.
ACCESSION   DASVOD010000302 DASVOD010000000
VERSION     DASVOD010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803083"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VF720_06780"
     CDS             <1..309
                     /locus_tag="VF720_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX7879096.1"
                     /translation="SILSGITRNAVMTLAADLGIEVREAPVPREMLYLADEVFFCGTA
                     AEITPIKSIDKIAIGAGRRGPITEKLQKAFFEIVRGETPDRHGWLTQVPAMAGAASGR
                     "
     gene            357..1604
                     /gene="glyA"
                     /locus_tag="VF720_06785"
     CDS             357..1604
                     /gene="glyA"
                     /locus_tag="VF720_06785"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010302450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX7879097.1"
                     /translation="MDWTLENLAAQDPAIADVLNAERRRQQTTLELIASENFVSTAVL
                     EAMGSVLTNKYAEGYPGKRYYGGCEHVDVAESLAIERAKSLFGAEHANVQPHSGSTAN
                     MTAFFSFLNPGDTILGMSLAHGGHLTHGHPVNFSGRFFRVLQYGLDPTTGRVDYDEVR
                     RLAKECQPKILLAGWSAYPRQLDFAAFRSIADEVGARFMVDMAHFAGLVAGGAHPSPV
                     PHADVVTSTAHKTLRGPRSGFILSKAEHASAIDKTNFPGMQGGPLMHMIAAKAVCFLE
                     ASKPEFKTYAARVVANAKALAEVMLARGYDLVTGGTDVHLILVDLRKKNLTGKKAEAI
                     LEEAGITTNKNAVPNDPQKPFVTSGIRVGTPAATTRGLGPEEFKIVGGLMADVLDAPD
                     DEAVRGRVRGRIEELGRAFPLYA"
     gene            1827..>3144
                     /locus_tag="VF720_06790"
     CDS             1827..>3144
                     /locus_tag="VF720_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879098.1"
                     /translation="MRHPLAVPALFALAIVVVALPGAEPTSPFTWLRALAALGLFGWF
                     PGWALERSLRLPRLEGFALAIAPIISTGLLVLTAINGIPVRKAAWVPVVVTALLMLVG
                     IARGSTGRRSTLGSSAGGGADASSDPHDSHLRWFALAVVALLVLINIVNPWLRWRADG
                     RFHIGVTAEFLRGGIPPTDPFFAGLNLQYMWFFHAWLAGLAALSGAATSWLMVGTNML
                     GLVAFLSLVAVLARDLGRSRNATWITVLVTAFGMGGLSWALLPVKLAGVFTGAKRGME
                     ELKTLFALWPPTIQKEMGFLSAGYDQPFLLRKFLVGTAMSLALAALMLQLVLVLRRLR
                     GGGALLLPLIALVSGGAIILHPMVGIPVVAVGIVAAIGAPLLPHVERLIGRLAPTTRG
                     ADAGAHTVPWSRALSVVIASVAGVALSMPYMRLVTAGKEGTGGLPID"
BASE COUNT          449 a         1082 c         1053 g          560 t
ORIGIN      
        1 tcgatcctgt cgggcatcac ccgtaacgcc gtcatgacgc tggccgccga cctcggcatc
       61 gaggtgcgcg aggcgcccgt gccgcgcgag atgctctacc tggccgacga agtcttcttc
      121 tgcggcaccg ccgccgagat cacgccgatc aagtcgatcg acaagatcgc catcggcgcg
      181 ggacgtcggg gcccgatcac cgagaaactc cagaaggcgt tcttcgagat cgtgcgcggc
      241 gagacgccgg atcggcacgg gtggctgacg caggtgccgg cgatggcggg ggcggcttcc
      301 ggccggtagc ggtccgcccg ttgggattcc acttccgaac ctgatagggt gccgccatgg
      361 actggactct tgagaatctc gccgcccagg accccgccat cgcggacgtc ctcaacgccg
      421 aacgccgtcg ccagcagacc acgctcgaac tgatcgcctc ggaaaacttc gtgtcgaccg
      481 ccgtcctcga ggcgatgggg tcggtgctga cgaacaagta cgccgagggt tatcccggga
      541 agcgctacta cggcggctgc gagcacgtgg acgtggccga gagccttgcc atcgagcgcg
      601 ccaagtcgct gttcggcgcc gagcacgcca acgtgcagcc gcattcgggg tcgacggcca
      661 acatgacggc cttcttctcg ttcctgaacc cgggcgacac aatcctcggc atgagcctgg
      721 ctcacggcgg ccatctcacg cacggccacc cggtcaattt ctccgggcga ttcttccgcg
      781 tgctgcagta cgggctcgac ccgacgacgg ggcgcgtgga ttacgacgag gtgcgccgcc
      841 tggcgaagga gtgccagccg aagatcctcc tggccggctg gagcgcgtat ccccgccagc
      901 tcgacttcgc cgcgttccgc tcgatcgccg atgaagtggg ggccaggttc atggtcgaca
      961 tggcgcactt cgcgggcctc gtggccggcg gggcgcatcc gtcgccggtc ccgcacgccg
     1021 atgtcgtgac gtccaccgcg cacaaaacgc tgcgtgggcc gcgctcgggc ttcatcctct
     1081 cgaaggccga gcacgcctcg gccatcgaca agacgaactt cccggggatg cagggcgggc
     1141 cgctgatgca catgatcgcc gccaaggccg tctgcttcct cgaggccagc aagcccgagt
     1201 tcaagaccta cgcggctcgc gtggtggcca acgccaaggc gctggccgag gtgatgctgg
     1261 cccgcggcta cgacctcgtg accggcggca ccgacgtgca cctgatcctc gtcgacctcc
     1321 gcaagaagaa cctgaccggt aagaaagccg aggccatcct cgaggaagcc ggcatcacga
     1381 ccaacaagaa cgcggtgccc aacgacccgc agaagccgtt tgtgacgagc ggtatccggg
     1441 tgggcacacc ggcggccacc acgcggggcc tcgggccgga agagttcaag atcgtcggtg
     1501 ggctcatggc cgatgtgctg gacgcaccgg acgacgaggc cgttcgcggg cgggtgcgcg
     1561 ggcgcatcga ggagctcgga cgggcgtttc cgctctacgc gtgagggcgt ccgcggcgcc
     1621 ccgggctgag ctttcccact gccgtggctt cacggggaac ctgttcgcaa tacggtcgtc
     1681 actttcaccg gtgaaagtgg cgggggaggg gacgtcgtca cgatccgacc ttcgttccct
     1741 gccgttgccg ggttcctttc atcggtgaaa gtaactccct tcgatactcc ttccgaattc
     1801 ccgctcctcc gctatcatcc cgcccgatgc gccatccgct agccgttccc gcgctcttcg
     1861 cccttgccat cgtcgtcgtg gcgctccccg gcgccgaacc gacgtctccg ttcacctggc
     1921 ttcgcgccct ggccgcactc gggctcttcg gctggtttcc gggctgggcg ctggaacgga
     1981 gcctgcggtt gccccggctg gaggggtttg cactcgcgat cgcgccgatc atcagcaccg
     2041 gcctcctcgt gctgacggcc atcaacggga ttcccgttcg gaaggcggca tgggtgcccg
     2101 tcgtcgtcac cgcgttgttg atgctggtgg ggatcgcgcg cggatcgacc ggcaggcggt
     2161 cgacgctggg gagttccgcg ggggggggcg ctgatgcctc ctccgaccct cacgactccc
     2221 accttcgctg gttcgcactg gccgtcgtgg cgctcctcgt cctcatcaat atcgtcaacc
     2281 cctggctccg atggcgggcc gacggccgct tccatatcgg tgtcacggcc gagtttctcc
     2341 gtggcggcat cccgccgacc gacccgttct tcgccgggct gaacctccag tacatgtggt
     2401 tcttccacgc ctggctggct ggtctcgcgg cgctgtccgg cgcggccacg tcgtggctca
     2461 tggtcggcac caacatgctg ggcctcgtgg ccttcctgtc gctggtggcc gtgctggccc
     2521 gggacctcgg gcgatcgagg aatgccacgt ggatcaccgt cctggtcacg gccttcggca
     2581 tgggtggact ctcctgggcg ctcctgccgg tcaagctcgc cggggtcttc accggcgcga
     2641 agcgggggat ggaggaactg aaaaccctct tcgccctctg gccccccacc atccagaagg
     2701 agatgggatt cctctcggcc gggtatgacc agccgttcct gctccggaaa ttcctcgtgg
     2761 gcacggcgat gtcgctggcg ctggcggccc tgatgcttca actcgtgctc gtgttgcggc
     2821 ggctgcgcgg cggcggcgca ctcctcctgc cgttgatcgc cctcgtatcc ggcggcgcca
     2881 tcattcttca cccgatggtg gggattccgg tggtggcggt cggcatcgtt gcggcgattg
     2941 gtgcgccctt gctgccgcat gtcgaaagac tcatcggtcg gctggcgccg acgaccaggg
     3001 gcgccgatgc gggcgctcac accgtcccct ggagccgggc cctctcggtg gtgatcgcct
     3061 ctgtcgcggg ggtggcgctc tcgatgccgt acatgaggct cgtcacggcg gggaaggaag
     3121 ggaccggcgg gttgccgatc gact
//
LOCUS       DASVOD010000303         3071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_803089, whole genome shotgun sequence.
ACCESSION   DASVOD010000303 DASVOD010000000
VERSION     DASVOD010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803089"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /gene="rpmI"
                     /locus_tag="VF720_06795"
     CDS             <1..155
                     /gene="rpmI"
                     /locus_tag="VF720_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX7879099.1"
                     /translation="KTASGKLKRGRSFHRHILTSKSRKRKRHLKRSALVHDGDAARIA
                     TLLPYS"
     gene            301..666
                     /gene="rplT"
                     /locus_tag="VF720_06800"
     CDS             301..666
                     /gene="rplT"
                     /locus_tag="VF720_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX7879100.1"
                     /translation="MPRAKGVVAGRERHKKTLKAAKGNYSGRSRLYKSAKETVMRGLQ
                     FAYEHRRLKKRDFRRLWITRINAAARLNGLSYSVFMNGLKKAQVNVDRKMLAELAVND
                     AAGFTALAEVARQAAATAA"
     gene            686..1708
                     /gene="pheS"
                     /locus_tag="VF720_06805"
     CDS             686..1708
                     /gene="pheS"
                     /locus_tag="VF720_06805"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX7879101.1"
                     /translation="MPGLIDDLEELRRQAERELAAADDPAALEAFRVAWLGRKAGRVT
                     GVLRGLKDVPLEERAAIGDAANRLKESLTALFETKLAEQGGGGDARPAGLDYSRPGLR
                     PPVGHTHLLTQTQESILDILTGMGFSPVSGPEVETVENNFDALNIGPEHPSRQPTDTF
                     FFGPDVLLRTHTSPVQVRAMRAQEPPIRICCPGRVYRREAVDATHLCEFHQVEILYVD
                     KGVTLRDLKGCLSTFARQMFGEATKTRLRPSYFPFVEPGAEVDVTCFLCQGSGCRVCK
                     GSGWIEILGAGMVHPNVLRNVGYDPDVWTGYAAGMGVERITMLRHGVTDIRHFQESDL
                     RFLEQF"
     gene            1733..>3071
                     /gene="pheT"
                     /locus_tag="VF720_06810"
     CDS             1733..>3071
                     /gene="pheT"
                     /locus_tag="VF720_06810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX7879102.1"
                     /translation="MKISLNWLRNYVDLPESPDVVGQRLTSLGFPVEGMETTGAPLDS
                     FVVGTVLAAERHPNADKLTLCTVDVGGESPLQIICGAPNAAVGVRAAVALIGAVLPDG
                     TKIKKARLRGVESQGMLCSERELGLSTEHNGIIDFGAAGAAPGTPLSEVLGAGDTLFD
                     IDIASNCGDGFSHVGVARELAAAFDRPLKLPETGLTEGNEPASGRIRVVVEDAAGCPR
                     YLARVITGLTVGASPEWLQKKLASIGQRPISNLVDVTNFILWELGQPLHAFDFDKLSG
                     NTITVRRAREHEMLTTLDGVERRLDDEVLIIADGERAVALAGIMGGKETEVTDATTTV
                     LLEGAVFDSYRTLKGAGVARLTTDASLRFVRGIDPAGVERAIDRCAALLAEVGGGTVL
                     SGRVEVTAADALAPRTVTWRHGAATLLIGEDIPDDEAGRWLTKLCFTAAPAAAP"
BASE COUNT          506 a         1073 c         1032 g          460 t
ORIGIN      
        1 ggaagaccgc cagcggcaag ctcaagcgtg gccgctcgtt ccaccgtcac atcctgactt
       61 ccaagagccg caagcgcaag cgtcacctga agcgctccgc gctggttcac gacggcgatg
      121 cggcgcgcat cgcgacgctg cttccgtata gctgaagtcg aaccgcagta gtcgaaccgg
      181 cgtgccggtt cgacatccct tcgtttttcg ttctgtgaca tcgttagaca ccaggtccaa
      241 tagataacat cggctgcagc tctcaccgag cccgcgagcc gtatcgtgga ggtacccgtc
      301 atgccaagag caaaaggcgt cgtcgccgga cgcgaacgac acaagaaaac cctcaaggcc
      361 gcgaagggca attacagcgg tcggagtcgc ctgtacaaga gtgccaagga aacggtcatg
      421 cgcgggctgc agttcgccta cgagcatcgc cgcctcaaga agcgcgactt ccgccgcctc
      481 tggatcaccc gcatcaacgc cgccgcccgg ctcaacgggt tgtcgtacag cgtcttcatg
      541 aacgggttga agaaggccca ggtgaacgtg gaccgcaaga tgctggccga actggccgtc
      601 aacgacgcgg ccggattcac cgcgctggcc gaggtggccc gccaggccgc cgcaaccgcc
      661 gcctgatccc gagccggagc cacccatgcc cggcctgatc gacgacctcg aggaacttcg
      721 ccgccaggcg gaacgtgaac tggccgccgc cgatgatccg gcggcgctgg aggcgttccg
      781 cgtggcctgg ctcggccgca aggccggccg ggtcaccggc gtgctgcggg gcctcaagga
      841 cgtgccgctc gaggagcgcg ccgccatcgg cgatgccgcc aatcgtctca aggagtcgct
      901 caccgcgctc ttcgagacga aactggccga gcaggggggc gggggagatg cccgccccgc
      961 ggggctcgac tacagccggc cggggcttcg tcctccggtg gggcacacgc atctcctcac
     1021 gcagacgcag gaatcgatcc tcgacatcct caccgggatg ggcttctcgc ccgtttccgg
     1081 gccggaggtc gagaccgtcg agaacaattt cgacgcgctg aacatcggcc ccgagcaccc
     1141 gtcgcgccaa ccgaccgaca cctttttctt cggcccggac gtgctcctgc gcacccacac
     1201 ctctccggtg caggtgcggg ccatgcgcgc ccaggaaccg ccgatccgta tctgctgccc
     1261 cgggcgcgtg taccgtcgcg aggcggtcga tgccacgcat ctctgcgagt tccaccaggt
     1321 ggagatcctc tacgtcgaca agggggtcac gctgcgggat ctcaaggggt gcctctcgac
     1381 cttcgcgcgg cagatgttcg gcgaagccac gaagacgcgg ctccggccgt cgtacttccc
     1441 gttcgtggag ccgggcgccg aggtcgacgt gacctgcttc ctctgccagg gctccggttg
     1501 ccgggtctgc aaggggagcg ggtggatcga gatcctcggc gccggaatgg tccatcccaa
     1561 cgtgctgcgc aacgtcggtt acgaccccga cgtctggacc ggctacgcgg ccggcatggg
     1621 tgtcgagcgg atcaccatgc ttcgccacgg cgtcaccgac atccggcact tccaggagag
     1681 cgatctccga ttcctcgaac agttctagga cccccatgac gaccggacgc gcatgaagat
     1741 cagcctgaac tggctccgca attacgtgga cctgccggaa tcgcccgacg tcgtggggca
     1801 gcggctcacg tcgctcggct ttcccgtcga gggaatggag acgaccggcg cccccctcga
     1861 ctcgttcgtg gtaggcaccg tgctggccgc tgagcgccat cccaatgccg acaagctcac
     1921 gctctgcacg gtggacgtgg ggggcgaatc gccgctccag atcatctgcg gcgcgccgaa
     1981 cgcggcggtg ggggtgcggg cggcggtggc gctcatcggc gccgtgctcc ccgacggtac
     2041 gaagatcaag aaggccaggc tgcgcggcgt ggaaagccag gggatgctct gctccgagcg
     2101 agagctcggc ctttccaccg agcacaacgg gatcatcgat ttcggggcgg cgggcgcggc
     2161 gcccggcacg ccgctctccg aggtgctggg cgccggcgac acgctcttcg acatcgacat
     2221 cgccagcaac tgcggcgacg gtttcagcca cgtgggcgtg gcgcgcgaac tggccgcggc
     2281 cttcgaccgg ccgctcaagc tcccggagac gggcctcacc gaagggaacg aaccggcttc
     2341 ggggcgtatc cgcgtggtgg tggaggatgc cgccggctgc ccgcgctacc tggcgcgcgt
     2401 catcacgggg ctcacggtgg gggcctcgcc cgaatggctc cagaagaagc tcgcctcgat
     2461 cgggcagcgc ccgatctcca acctggtcga cgttaccaac ttcatcctgt gggagctggg
     2521 gcagccgctc cacgccttcg acttcgacaa gctgtcgggg aacaccatca cggtccggcg
     2581 cgcgcgcgag cacgagatgc tcaccacgct cgacggcgtg gaacgacgcc tcgacgacga
     2641 ggtgctgatc atcgccgacg gggagcgcgc cgtggccctt gccggcatca tggggggcaa
     2701 ggaaaccgag gtcaccgacg ccacgaccac ggtgctcctc gaaggcgcgg tcttcgactc
     2761 gtatcggacg ctcaaggggg ccggcgtggc ccgcctcacc accgacgcct cgctccgctt
     2821 cgtgcgcggc atcgatccgg ccggcgtcga gcgggccatc gaccgctgcg cggcgctcct
     2881 ggccgaggtg ggggggggca ccgtcctttc cggtcgcgtg gaagtgacgg cggccgacgc
     2941 gctcgccccg cgcaccgtga cgtggcgcca cggcgccgcc actctcctga tcggcgagga
     3001 catcccggac gacgaggccg gccggtggct cacgaaactc tgcttcacgg ccgcgccggc
     3061 ggcggcgccc g
//
LOCUS       DASVOD010000304         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_541511, whole genome shotgun sequence.
ACCESSION   DASVOD010000304 DASVOD010000000
VERSION     DASVOD010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541511"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VF720_06815"
     CDS             complement(<1..227)
                     /locus_tag="VF720_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879103.1"
                     /translation="MNQTSSTRGLPPGRARLLAPAALIFLTLTAGCGKHDVRSYQVPK
                     EMVPTGANHPALSAPPPPAETVEVPHWKLPSG"
     gene            498..1931
                     /locus_tag="VF720_06820"
     CDS             498..1931
                     /locus_tag="VF720_06820"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7879104.1"
                     /translation="MFRPRLLSSLLVGAIAITLSLAACSDEETVYVERPLFEDPPDSA
                     QGFLGFDDQATKLTVCGNCHVGQQAKWEGTHHSDAWATLQESGHAQGFCNDCHSVGPN
                     GNNTSGSVGYAAVADMRYHDVQCESCHGPGETHVSNPDASQPQASIAILDGNEPTKNC
                     GECHTGEHHPFAIEWAKSRHAKFNNSAKNSTSTECLSCHSGQGVLAAWGVTDNYIEKN
                     APRAEHVGIVCTVCHDPHARNNPAQLRFPIDVPDESLNLCMKCHNRRSTPEVASASLR
                     GPHAPEGPLLLGTAGWWPPGFEPEIDRIVATHGTTGNPRLCATCHVSSFDVTDPETGN
                     FVFSATGHLFKAIPCLDEQGVPLPEEECDVTERAFDSCAQSGCHGTANSARSAFLTAN
                     ARMENLVDEVRRLLTLVPPGEFNLTDGRFTVADGAWFNAQLASAKGSPTHNPFLTEQL
                     LVASIDALENEYGVTAAPGVSRDRMFR"
     gene            1993..3018
                     /locus_tag="VF720_06825"
     CDS             1993..3018
                     /locus_tag="VF720_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879105.1"
                     /translation="MAVLILSCARPGSEAPPTGPGPVSGSVAASACPACAALRSSPSA
                     PGGVADTLGTDEAHPPADPHGTNCAVCHHPGGRHLSDTHDWLPTCTTSECHPKAWTET
                     VFHRVEPAVFRDCTNCHRPHTWKASGEDCRSCHGPRGTPAAVTPMAAFPHERHESLAC
                     TTCHAVGEGRHATPASPDPARCQSCHHGPPAVASCGNCHAPDELAGRREVPVMLTLAR
                     NAAPRSRRLGFDHARHRELACATCHAAGHETPVRVDCASCHRRHDLTHAACGTCHEAP
                     PAGAHRLALHETGCAGTACHDDIGLTRLTDRREVCLACHADQVRHQAGKVCASCHLVS
                     LGHGAGS"
     gene            3015..4487
                     /locus_tag="VF720_06830"
     CDS             3015..4487
                     /locus_tag="VF720_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879106.1"
                     /translation="MNLRRPLGLSAMIVVGTLIRMSEAEAQGIRGEYQVSASAIEYLS
                     VVRDSFPESEVPGSGTTRVLPDGTVVECVPGGYCYRYRAGELRTASPVTEDLSLTAWP
                     GWRGIQARAHVRGRFGSNDLWPRSSEKLALLDLTVEMDRTDWRLRAGRQQHLGGLGAM
                     HFDGGSLLWKGVPAVRVTAFAGRSLGRGLFAPYTGSLLAESDELPPLREAVVVGVEGR
                     YRPNATLAASLLYQVEIRTDRASLYSERVALDATWQPQLLRLELSGDADLASLVVNDL
                     KLRLGRTFGTHLDLTVEGRHSEPFFELWTIWGAFTPVGFDQLRFSADYRIDRNLSLEG
                     GIAWRDYAETHTGIQLAPIEGDGLRGRAGARWQQEAWSASVAAGLDQGYGAYRGQLDA
                     AVEREFGPRTSLGLFAVGTQQFTEFRFGEGTTRGVGATARHGIGAVDLTGRVGYDRHT
                     FENRPGYDDYGQWRGTIALSGRFGRDPAAPKPRDTPGWRP"
     gene            4484..>5119
                     /locus_tag="VF720_06835"
     CDS             4484..>5119
                     /locus_tag="VF720_06835"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX7879107.1"
                     /translation="MKHRIVAGATGLLLLMSGTPAFPEPRASSFPHARHARLFTACTA
                     CHDMAPTDAPERLVKVTAADCARCHDGNSAPVVGWSPSRTEAPNLQFSHERHVGREGA
                     DCLTCHAAPRQDPVTGGPAAGHGSPPAARMPDIVRPTAETCLACHQAAGHLSADAVCS
                     RCHDPLSNASALTVERIGAFPHPPNHEAADFLWAHGELAERDLARCAVCHAK"
BASE COUNT          775 a         1840 c         1707 g          797 t
ORIGIN      
        1 ccactcggga gcttccagtg cggcacctct accgtttcgg ccgggggagg cggagcggac
       61 aaggcaggat ggttggcgcc cgtcggcacc atctccttgg gcacctggta gcttcggaca
      121 tcgtgcttcc cgcacccggc cgtgagggtg aggaatatca gggcggcggg cgcgaggagg
      181 cgcgcccggc cgggcggaag gcctctcgtc gagctggtct ggttcatcgg gggcaggact
      241 cctgtcttgg cggggcaggc cggcggcgcg acgcgatcca ggccgcgatc ggctccgggg
      301 aattctaggg cttcaccggt gtggcgaaca ggtcgcctga tcgccacccc ggcattctct
      361 ccggtcgccc gatttgcacc ctgataattg tcggggttcc agagaaatca cgcttgtggc
      421 cgtcccgatc cccgcgatat acctccaagt ggtcgcaggt cgagaacccc ctcgaccgcg
      481 ccgcctcgga gggtcccatg ttccggccca ggttgttgtc gtccctgctc gttggcgcca
      541 tcgccatcac tctctccctc gcggcctgct ccgatgaaga gaccgtctac gtcgagcggc
      601 ccctcttcga ggacccgccc gattcggccc agggattcct cggtttcgac gaccaggcca
      661 ccaagctcac cgtctgcggc aattgtcacg taggccagca ggccaagtgg gaaggaacgc
      721 atcattccga cgcctgggcc actctccagg agtccggtca cgcgcagggc ttctgcaacg
      781 attgtcactc ggtcggcccc aacggcaata acacctcggg ctcggtcgga tacgcggccg
      841 tggccgacat gcgttaccac gacgttcagt gcgaaagctg tcacggcccc ggcgagaccc
      901 acgtgtccaa tccggacgcc agccagccgc aggcctcgat cgccatcctc gacgggaacg
      961 aaccgacgaa gaactgcggc gagtgccata ccggcgaaca tcatccgttt gccatcgaat
     1021 gggccaagtc gcgccacgcc aagttcaaca acagcgcgaa gaacagcacc tccaccgaat
     1081 gcctcagctg ccattccggc cagggtgtgc tggccgcgtg gggggtcacc gacaactaca
     1141 tcgagaagaa cgcgccccgg gccgagcatg tcgggatcgt ctgcacggtg tgccacgatc
     1201 cgcacgcgcg gaacaacccg gcgcagctgc ggttccccat cgacgtgccg gacgagagcc
     1261 tcaacctctg catgaagtgc cacaaccggc gttccacgcc cgaagtggcc tccgcctcgc
     1321 ttcgtgggcc gcatgctccc gaaggaccgc tgctcctcgg taccgccggc tggtggcctc
     1381 ccggcttcga gccggagatc gaccggatcg tggccacgca tggcaccacc ggcaacccgc
     1441 gcctgtgcgc cacgtgtcac gtctcctcgt tcgacgtcac cgatcccgag accggcaact
     1501 tcgtcttcag cgccacgggt cacctcttca aggccatccc ctgcctcgat gaacagggcg
     1561 tgccgctccc cgaggaggag tgcgacgtga cggagcgggc gttcgattcg tgcgcgcaga
     1621 gcggctgtca cggcacggcc aattcggccc ggtcggcctt cctcacggcc aatgcgcgca
     1681 tggagaacct ggtggatgaa gtgcggcgtc tgctgaccct ggtgcctccc ggcgagttca
     1741 acctcaccga tggccgcttc acggtggcgg atggtgcctg gttcaacgca cagctggcca
     1801 gtgccaaggg atcgccgacg cacaacccgt tcctgacgga gcagttgctc gtggccagca
     1861 tcgatgcgct cgaaaacgag tacggcgtga cggcagcacc gggagtctcg cgggaccgga
     1921 tgttccggta accactccac ggtgacccgg agccgtctcg ccgattggaa atcggggttc
     1981 ggggcggcct ccgtggccgt cctcatcctg tcatgcgcgc gtcccgggtc ggaggcaccg
     2041 cccaccggcc cgggacctgt ttccggcagt gtggcggcgt ccgcctgtcc tgcatgcgcg
     2101 gccctgcggt catcgccgtc cgcgccgggc ggcgtcgccg atacgctcgg aacggacgag
     2161 gcgcatcccc ccgccgatcc gcacggaacg aactgcgccg tctgccatca tccgggtggg
     2221 cggcaccttt cggacacgca tgactggctc cccacctgca cgacgtcgga gtgccatccg
     2281 aaggcctgga ccgagacggt ctttcatcgc gtggagccgg cggtcttccg cgattgcacc
     2341 aactgtcacc gaccgcacac atggaaggcg tcgggcgagg attgtcggtc gtgtcacggc
     2401 ccgcgcggca cacctgccgc cgtcacgccg atggcggcat ttccgcacga acggcacgag
     2461 tcgctggcct gcaccacgtg ccatgcggtc ggtgaagggc gtcacgcgac accggcctca
     2521 cccgacccgg ctcgctgcca gtcgtgccac cacggccctc ccgcggtggc gtcgtgcggc
     2581 aactgtcacg cacccgacga gctggccggc cgtcgcgagg tcccggtgat gctgacgctc
     2641 gcgcggaatg cggcgccccg cagcaggcgc ctgggcttcg atcacgcgcg gcatcgtgaa
     2701 ctggcctgcg ccacctgcca cgcggccggt cacgaaactc ccgtccgcgt cgactgcgcc
     2761 agctgtcatc gtcgccacga tctcacccac gcggcctgcg ggacctgtca cgaggcgccg
     2821 ccggccgggg ctcatcggtt ggccctgcac gagaccggtt gcgccggaac agcctgccac
     2881 gacgacatcg gcctcacccg cctgaccgac cggcgggagg tgtgcctggc ctgccatgcc
     2941 gaccaggtgc gtcaccaggc cggcaaggtg tgcgcgtcgt gccatctcgt ctcgctggga
     3001 cacggggcgg gatcatgaac ctgcggcgcc cactgggcct ctccgccatg atcgtcgtcg
     3061 gcacgctcat acgcatgtcg gaggccgagg cccaggggat ccgtggcgaa taccaggtca
     3121 gcgcgtcggc catcgagtac ctctccgtcg tccgcgacag ctttccggaa tccgaggtgc
     3181 cggggagcgg gaccacccgc gtcctccccg acggaacggt ggtggaatgc gtgccgggag
     3241 gctactgcta tcgctaccgg gcgggcgagc tccggaccgc atcccccgtc accgaggacc
     3301 tctcgctgac tgcctggccg gggtggcgtg gcatccaggc ccgggcgcat gtgcgcgggc
     3361 gcttcggcag caacgacctg tggcccagaa gcagcgagaa actggcgctg ctcgacctga
     3421 ccgtcgagat ggaccgcacc gactggcgcc tgcgggccgg ccggcaacag cacctggggg
     3481 ggctcggcgc catgcacttc gacggtggga gcctcctctg gaaaggggtg ccggccgtcc
     3541 gcgtgacggc cttcgcgggg cggagcctgg ggcgtggcct ctttgcgccg tacacgggct
     3601 cgctcctggc cgagtcggac gagctccccc ccctgcgcga ggccgtggtg gtcggcgtcg
     3661 agggacgtta ccgaccgaat gccacgctgg ctgcctcgct cctctaccag gtggagatcc
     3721 ggacggatcg cgcatcgctc tacagcgagc gtgtggcgct cgacgccacc tggcagccac
     3781 agcttctgcg actcgagctg agcggtgatg ccgacctggc ctcgctcgtc gtgaacgacc
     3841 tgaagctgcg gctcggccgg acgttcggaa cgcacctcga cctcacggtg gagggtcgac
     3901 actccgaacc cttcttcgag ctctggacga tctggggcgc cttcaccccg gtgggcttcg
     3961 atcagctccg cttttcggca gactatcgaa tcgaccgcaa cctctcgctc gagggtggta
     4021 ttgcctggcg cgactacgcc gagacgcaca cggggatcca gctggcgccg atcgaggggg
     4081 atggcctccg cgggcgcgcc ggcgcgcgat ggcagcagga agcctggagc gcctcggttg
     4141 ccgcgggact cgaccagggg tatggcgcct atcgcgggca gctggacgcc gcggtcgagc
     4201 gtgagttcgg cccgcgaacc agccttggat tgttcgcggt cggcacccag cagttcaccg
     4261 aattccgatt cggcgaaggg acgacgcgag gagtgggcgc cacggcgcgc cacgggatcg
     4321 gcgcggtcga tctcaccggg cgcgtcggtt acgaccggca caccttcgag aatcgtcccg
     4381 gctacgatga ctacggccag tggcggggga cgatcgccct ctccggacgt ttcggtcgcg
     4441 atcccgccgc gccgaagccg cgcgataccc cgggatggcg gccatgaagc accggatcgt
     4501 ggccggcgcg accggcctcc tcctgttgat gtcgggcacg cccgccttcc ccgagccacg
     4561 agcgtcctcg tttccccacg cgcgtcacgc gcgcctcttc accgcgtgca cggcgtgcca
     4621 cgacatggcg ccgaccgacg cgccggagcg cctggtgaag gtgactgccg ccgattgcgc
     4681 gcgctgccac gacggcaact cggcgccggt cgtcggctgg agcccgtcgc ggaccgaggc
     4741 gccgaatctg cagttttcgc acgagcgtca cgtcgggcgc gaaggagccg actgcctcac
     4801 gtgccacgcg gcgccccgac aggatcctgt cactggcgga ccggcagccg gccacgggag
     4861 cccgccggcc gcccgcatgc ccgacatcgt gcgaccgacc gccgaaacct gccttgcctg
     4921 ccaccaggcc gccgggcacc tgtcggccga tgccgtctgc tcgcgctgtc acgacccgct
     4981 gtcgaacgcg tcggccctca ccgtcgagcg gatcggtgcg tttccccatc cccccaatca
     5041 cgaggccgcc gatttcctgt gggcccacgg cgaactggcg gaacgtgacc tggcgcgctg
     5101 cgccgtgtgc cacgcgaag
//
LOCUS       DASVOD010000305         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_698122, whole genome shotgun sequence.
ACCESSION   DASVOD010000305 DASVOD010000000
VERSION     DASVOD010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698122"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF720_06840"
     CDS             complement(<1..597)
                     /locus_tag="VF720_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010962192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7879108.1"
                     /translation="MSTDLDAARKRAATTYNAAADTYDDPANTFWSRFGRATVDRLAL
                     RSGDLVLDVCCGTGASAIPAAEAVGPTGRVLAIDLATQLLERGRARAAAQDLDNIEFR
                     EGDLLDPALPEWGFDVVVCVFGIFFVPEMEKAVAALWRLVKPGGRLAITTWGPRLFEP
                     ANSAFWDSVKDVRPELHKGFNPWDRITTPTSLAQLLRDG"
     gene            complement(675..1037)
                     /locus_tag="VF720_06845"
     CDS             complement(675..1037)
                     /locus_tag="VF720_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7879109.1"
                     /translation="MIRGMHAMFYSDQAAELRAFLRDKLGLAGTDVGQGWLIFDAPEA
                     DLGVHPTEGEGEGPPSGTADISFYCDDIAATVAELTARGVEFTQPVEDHGYGLVTHMR
                     VPGGFTVQLYQPRYAKSK"
     gene            1314..2120
                     /locus_tag="VF720_06850"
     CDS             1314..2120
                     /locus_tag="VF720_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HEX7879110.1"
                     /translation="MAHVATALPARRRFAETMRRDAWWVEPALTFLVLAAFLTYANWA
                     AFQNAHYTYGNYLSPFYSPELWGSSHHAWFGPKPAWWPGFLPFSPALLVLWAPGLFRL
                     TCYYYRGSYYKAFIADPASCAVGEPKRNYKGETGFPLILQNLHRYFMYLALPFLLILA
                     FDVWKALWFENPTTGRTEFGIGLGTLILAVNVVLLSGYVLGCHSFRHWIGGKMDCLGK
                     RPLRRKAYDCVSCLNRGHMKWAWPSLFWVAFSDIFIRLCSMGIWHDWRIL"
     gene            2185..>3669
                     /locus_tag="VF720_06855"
     CDS             2185..>3669
                     /locus_tag="VF720_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX7879111.1"
                     /translation="MSTDVTTVEHDVLIVGAGGAGLSAAIQASGKGAKVGVICKSLLG
                     KAHTVMAEGGIAAAMANVDERDSWKVHFADTMRGGQYLNHWRMAELHAKEAPAAVKEL
                     EAFGALFDRTKDGRILQRNFGGHRYPRLAHVGDRTGLEMIRTLQDHGIHQGMDVHMEV
                     TVIAILTDGGRVSGALAYDRERGYFKLFKTKAIVLATGGVGRAFRVTSNSWEYTGDGQ
                     ALAYEAGAELQDMEFVQFHPTGMVWPPSVQGILVTEGVRGEGGVLRNRDGKRFMFDDI
                     PENYRGQTADNEEEGWRYTQGDRNARRPPELLTRDHVARCIMREVKAGRGSPRNGVYL
                     DISWIKEKLPNAAEHIRKKLPSMYHQFKQLANIDITKEPMEVGPTTHYIMGGVRVDPD
                     SQMSCVPGLFAAGECAAGLHGANRLGGNSLSDLLVFGRRAGIAAAEFATASKPGAIND
                     AEVKRHIAAAVEPFERGNSAGAESPYALQKELQDIMQDEVGIVRN"
BASE COUNT          618 a         1238 c         1215 g          598 t
ORIGIN      
        1 gccatcccgg agcagctggg cgagcgaggt cggcgtggtg atccgatccc acgggttgaa
       61 tcccttgtgc aattccggcc ggacatcctt gaccgaatcc cagaacgccg agttcgccgg
      121 ctcgaagagt cgcggacccc atgtcgtgat ggcaagccgg ccgcccggct tcaccagccg
      181 ccacagggcc gccacggcct tttccatctc cggcacgaag aagatgccga agacgcagac
      241 gacgacgtcg aacccccact ccgggagcgc ggggtcgagg agatcaccct cacggaactc
      301 gatgttgtcg agatcttgcg cggccgcgcg ggcacgtccg cgctcgagaa gttgcgtggc
      361 caggtcgatg gccaggacgc gcccggtcgg gccgacggcc tcggcggccg gaatggcgct
      421 ggcgccggta ccgcagcaga catcgaggac cagatcgccc gagcgaagtg ccaggcgatc
      481 gacggtggcc cggccgaagc gtgaccagaa tgtgttggcg gggtcgtcgt acgtgtcggc
      541 ggcggcgttg taggtggtgg cggcgcgctt tcgtgcggcg tcgaggtcgg tggacatccg
      601 gtcgggtctc cgtggggtcg gtgcggtcgg ggcgttgtca acgagggtgt tcggctaacc
      661 atcccgccgt cctgctattt cgacttcgcg tacctcggct ggtagagctg caccgtaaac
      721 ccgcccggca cccgcatgtg cgtgaccaga ccatagccat gatcttcgac tggctgggtg
      781 aactccacgc cacgggcggt cagctcggcc accgtggcgg caatgtcgtc gcagtagaac
      841 gaaatgtcgg ccgtgcccga cggcggaccc tccccttccc cttccgtggg atgcacgccc
      901 aggtcggcct ccggcgcgtc gaagatgagc cacccctggc cgacatcggt tccggcgagt
      961 ccgagcttgt cgcgcagaaa ggcgcgcagc tcggcggcct ggtcggagta gaacatggcg
     1021 tgcatgccgc ggatcatggg tacctcctgg aggtccgtcg tccaataacg ggacggcatg
     1081 actgtatcaa caacaggtcc acgactctgt ccgaaccgag ttgccagcgg ccctcgactc
     1141 tgtccgaacc gggttgccag cggccctcga ctctgtccga accgggttgc ccgtcacggt
     1201 agcgcgcccc gcaccccgcc gctatactgc cgcccgtcgc gagttcgacg ttccgttcac
     1261 cggcacgccc ggcgtgatcc ggtgccacga caggcacttc ggagagatgt ccgatggctc
     1321 acgtcgctac agctttgccg gcccgccggc gcttcgcgga aacgatgcgg cgcgacgcgt
     1381 ggtgggtcga accggccctc acgttcctcg tcctggccgc cttcctcacc tacgcgaatt
     1441 gggccgcatt ccagaatgcc cactacacgt acgggaatta cctgtcgcct ttctactcgc
     1501 ccgaactctg gggctcctcg catcacgcct ggttcggccc caagcccgcc tggtggcccg
     1561 gattcctgcc gttctctccc gccctcctcg tgttgtgggc gcccggcctc ttccgactca
     1621 cctgctatta ctatcgcggc tcgtactaca aggcgttcat cgccgacccg gcctcgtgcg
     1681 ccgtgggcga accgaagcgg aattacaagg gtgaaacggg cttcccgctg atcctccaga
     1741 acctgcaccg ttacttcatg tatctcgccc ttcctttcct gctcatcctc gccttcgacg
     1801 tctggaaggc cctctggttc gagaacccga ctaccgggcg cacggagttc ggcatcgggc
     1861 tgggcacgct catcctggcg gtcaatgtgg tgctgttgtc gggctacgtc ctgggctgcc
     1921 actcgttccg ccactggatc ggcgggaaga tggactgcct cggcaagcgt ccgctccggc
     1981 gcaaggcgta cgactgcgtg tcgtgcctga accgcgggca catgaaatgg gcctggccaa
     2041 gcctcttctg ggtggccttc agcgacatct tcattcgcct ctgctcgatg gggatctggc
     2101 acgactggag gatcctgtga tgaatgcagg tctccgaacc gacggcgctc ctgagtcggc
     2161 aacaggccat atccggagct gccgatgagc accgacgtca ccaccgtcga gcacgacgtc
     2221 ctcatcgtcg gcgccggcgg cgccggcctc tccgccgcca tccaggcttc gggaaaaggc
     2281 gcgaaagtcg gcgtcatctg caagtcgctg ctcggcaagg cgcacaccgt gatggccgag
     2341 gggggcatcg ccgccgccat ggccaacgtc gatgagcgcg actcctggaa agtgcacttc
     2401 gccgacacga tgcgcggcgg gcagtacctg aaccactggc ggatggccga gctgcacgcc
     2461 aaggaagcgc cggccgcagt gaaggaactg gaagccttcg gcgcactgtt cgatcgcacg
     2521 aaggacggtc gcatcctcca gcgcaacttc ggcggccacc gctacccgcg cctggcccac
     2581 gtgggcgatc gcaccggcct cgagatgatc cgcacgctac aagatcacgg catccaccag
     2641 gggatggacg tgcacatgga agtgaccgtc atcgccatcc tgaccgacgg cgggcgggtg
     2701 tcgggggcgc tggcctacga ccgcgagcgc ggctacttca agctgttcaa gacgaaggcg
     2761 atcgtgctgg ccaccggcgg cgtggggcgg gcgttccgtg tcacgagcaa ttcgtgggag
     2821 tacacgggcg acggccaggc gctggcctac gaggccggcg ccgagctcca ggacatggag
     2881 ttcgtgcagt tccaccccac cgggatggtg tggcccccca gcgtgcaggg gatcctcgtc
     2941 accgaggggg tgcgcggcga ggggggggtg ctccgcaaca gggacggcaa gcggttcatg
     3001 ttcgacgaca tccccgagaa ctaccgcggc cagaccgccg acaacgagga agaagggtgg
     3061 cgctacacgc agggggatcg gaacgcccgg cggccgcccg aactcctcac ccgcgaccac
     3121 gtggcgcgct gcatcatgcg cgaggtgaag gccggccgcg ggagtccgcg caacggggtg
     3181 tacctcgaca tctcctggat caaggaaaag ctgccgaacg ccgccgagca catccggaag
     3241 aagctcccga gcatgtatca ccagttcaag cagctcgcca acatcgacat cacgaaggaa
     3301 ccgatggaag tcgggcccac cacgcactac atcatgggcg gcgtgcgggt cgatccggac
     3361 agccagatgt cgtgcgtgcc gggactcttc gccgccggcg agtgcgcggc ggggctgcac
     3421 ggggccaacc gactcggcgg caattcgctg tccgacctgc tggtctttgg ccgccgtgcc
     3481 ggcatcgcgg ccgccgagtt cgccacggcc agcaagcccg gcgcgatcaa cgacgccgag
     3541 gtcaagcggc acatcgccgc ggccgtcgag ccgttcgagc gtggcaactc ggccggcgcc
     3601 gaatcgccgt atgcgctgca gaaggaattg caggacatca tgcaggatga ggtggggatc
     3661 gtgcgcaac
//
LOCUS       DASVOD010000306         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_282294, whole genome shotgun sequence.
ACCESSION   DASVOD010000306 DASVOD010000000
VERSION     DASVOD010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282294"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VF720_06860"
     CDS             complement(<1..330)
                     /locus_tag="VF720_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7879112.1"
                     /translation="MLTPVERVLILKGADLLKDVGPRPPLGLAEVARERDIKAGETIY
                     TEDDPADSLYMVVDGRVRLAAAGRTISEVGPGEAFGTWALVDDSSRGQTAVAEEDGLV
                     LALHRDEF"
     gene            complement(324..>2083)
                     /locus_tag="VF720_06865"
     CDS             complement(324..>2083)
                     /locus_tag="VF720_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7879113.1"
                     /translation="FVAMRWIQGVVFQTLGKSSSEIYFAAIQPTERRLVKPAIDTLVE
                     RWSDALAGILLIVALRILHVPIGVIAGITVVLAGAWLLVLLRLNHDYGRAFEEALSTR
                     WMSSDTPPEVIHTPSARKALLDGLLQNDERRIVLALRLAGQSGARGRLASAIRACLRN
                     KAPSVRATALQTMQALRLADTDGAIPALLNDPEDVVRRAAVAYQLSMSPKPGELARSY
                     LEGDDPALRQYVLDALWTYPREIRSAVSLEWIDRLMASPSGDDRVLAARALGTISGRP
                     AADRLARLLDSEDPEVRTAALLSATRRPHPILLDRLIKLLREPSCHVEARRAVSMLGN
                     LAVPRLAAMLEPDRPSQERGAAARTLAEIATPRAVRVLMTLVRSVDIDRRYLGLRHLA
                     RARAVVGRPILARDLVHRLFLRELRDYRACLDAATSLEPAGFPEVKLLGESYRESGVT
                     ALERALLALAAFYEPRPLFGVFHRLASNNPDLAAPALEFLGHALPRGVFRPVMTMFDR
                     RTTPNTDGPTELERIEGWIQSAWASSDDWLRACALRAAHTLPGIDTAPFRPGAADGPI
                     VSAELGLFPVTAPGAGKSC"
BASE COUNT          332 a          695 c          776 g          280 t
ORIGIN      
        1 gaactcgtcg cggtggagcg ccagcaccag gccatcttcc tcggccacgg ccgtctggcc
       61 gcgactggag tcgtcgacca gcgcccatgt gccgaaagcc tcgcctggac ccacctcgga
      121 gatcgtgcgt ccggcagcgg caagcctcac gcggccgtcg acgaccatgt aaagcgagtc
      181 ggccggatcg tcctcggtgt agatcgtttc gccggccttg atatcgcgct cgcgggccac
      241 ctcggccagg ccgagcgggg ggcgcgggcc gacatccttg agcaggtcgg ctcccttgag
      301 gatgaggacg cgttcgaccg gtgtcagcat gacttgcctg ccccaggagc ggtcaccggg
      361 aagagcccga gctccgctga aacgatgggg ccgtccgcgg cgccggggcg gaacggggcg
      421 gtgtcgatgc cggggagggt gtgggccgcg cgcagggcac aggctcgcag ccagtcatcg
      481 ctcgaggccc atgcggattg gatccacccc tcgatccgtt cgagttcggt cgggccatcg
      541 gtattcggcg tggtgcggcg gtcgaacatg gtcatgaccg gtcggaacac cccccggggg
      601 agcgcgtgtc cgaggaattc gagggccggc gcggccaggt cgggattgtt ggacgcgagg
      661 cggtggaaca cgccgaagag cgggcggggc tcgtagaaag cggcgagggc caggagcgcc
      721 cgctcgaggg cggtcacccc ggactcgcga tacgactcgc cgagcagctt cacctcgggg
      781 aacccggccg gctcgaggct ggtggcggca tcgaggcagg cccggtagtc gcgaagctca
      841 cgcaggaaga gacgatggac caggtcgcgc gcgaggatcg gccggcccac caccgcccgc
      901 gcacgggcca gatggcggag ccccaggtag cgccggtcga tgtcgacact ccgaaccagg
      961 gtcatcagga cccggacggc ccgtggcgtg gcgatttcgg ccagcgtgcg cgcggccgcc
     1021 ccccgctcct gcgaaggccg gtcgggctcg agcatggcgg cgagacgggg caccgccagg
     1081 ttgcccagca tcgatacggc ccggcgggcc tcaacgtgac agctcggttc gcgcagcagc
     1141 ttgatgaggc gatcgagcag gatgggatgg ggccgacgcg tggccgagag aagcgcggcg
     1201 gtccggacct cggggtcttc cgaatcgagc aggcgggcca gccgatcggc cgccgggcgg
     1261 ccactgatgg tgccgagggc gcgggcggcg aggacgcgat cgtcgcccga cgggctggcc
     1321 atcagccggt cgatccattc gaggctcacg gcactccgga tttcgcgggg gtatgtccag
     1381 agggcatcga gcacgtactg ccggagagcc gggtcgtcgc cttccaggta gctgcgcgcc
     1441 aactcgcccg gcttcgggct catgctgagc tggtaggcca ccgccgcccg ccgcacgaca
     1501 tcctccgggt cgttgagaag cgcgggaatc gcgccgtccg tatcggccaa ccggagggcc
     1561 tgcatcgtct ggagggctgt cgcgcgcacc gacggcgcct tgttgcggag gcaggcccgg
     1621 atggccgagg ccagccgccc gcgggcaccg gactgcccgg ccaggcgaag ggccaggacg
     1681 atccgtcgct cgtcgttctg caggagcccg tcgagcaggg ccttgcgggc cgacggcgtg
     1741 tggatcacct cgggcggcgt gtccgacgac atccagcgcg tgctcaacgc ctcctcgaac
     1801 gcgcgaccgt agtcatgatt gagccggagc agcacgagca gccaggcgcc cgccagcacg
     1861 acggtgatgc cggcaatcac gccgatcggg acgtgcagaa tccggagcgc cacgatcagg
     1921 aggatgcccg ccagggcatc cgaccagcgc tcgacgagcg tatcgatggc cggcttcacc
     1981 agtcgtcgct ccgtcggctg gatcgccgcg aaatagattt cggacgacga cttcccgagc
     2041 gtctggaaga ccacgccctg gatccatcgc atggccacga agg
//
LOCUS       DASVOD010000307         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_907934, whole genome shotgun sequence.
ACCESSION   DASVOD010000307 DASVOD010000000
VERSION     DASVOD010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907934"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VF720_06870"
     CDS             complement(<1..409)
                     /locus_tag="VF720_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879114.1"
                     /translation="MSDILLRMPNWVGDAVMALPALEALHEIRPGSAIDVMAHPRVRP
                     LVESIAWIRDLPPVPGRGDAGWREVIRTLRRRRYALGITLAPSFSSAFVLAAGGVRER
                     LGRKGGGRDFLLTTALSAGVRGHPQVTQYVDVVR"
     gene            complement(406..>1846)
                     /locus_tag="VF720_06875"
     CDS             complement(406..>1846)
                     /locus_tag="VF720_06875"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879115.1"
                     /translation="AALLLLPLVIGAGGAGPALRIILVSVAAFAVTSPAIVLNLPDAW
                     RAVTYERFHMTTGHFRAVNEAGWLFYLRDLAPRALGWLGWGAGLAGIVAALMAGGAGR
                     LVALFALAGLALLGSWRVAFDRYFLLLLPPLAVLAGTILGLAPRRIRSSDRMGLVTGL
                     VVLALAAGPTLVTSWQEGTRRARPSTRDAAAEWARGAIPKGSLVAAERYSIESMSDSL
                     ALLLIPFDSVEPHRYDPAYSLPYYAPFEWIVLSSALGDRYLSRIDEFPAQSAFYDGVA
                     RHLTLVAEFRPERGRVGPLIRIFRREPGGTLSDFRGIDPKFYDTLPDRAAMADFMRTL
                     AGVLARGGRSDLALPAAEQAVTLAPGDVKALTNLAILRGERGEYLSALNTYQRALAIA
                     PAEPRLHYNLGRLYESRELWREAAASYRRAADLAPSMIEAWWGLYAAQIHLDDRSGAA
                     GSLKRILDQLPPGPRADEVRRLLRSVEGP"
BASE COUNT          311 a          656 c          657 g          222 t
ORIGIN      
        1 cgcgaaccac gtcgacatat tgcgtgacct gcggatgccc gcgcacgccc gcggaaagag
       61 ccgtggtgag gaggaagtcg cgaccacccc ccttccgacc caggcgctcc cggacgccgc
      121 cggccgccag cacgaacgcc gacgagaagg atggggccag ggtgattccc agcgcgtagc
      181 gtcggcggcg aagcgtccgg atcacctccc gccagccggc gtcgccgcgt cccggcaccg
      241 gcgggaggtc gcggatccac gcgatcgatt cgaccagcgg ccgcacgcgg ggatgagcca
      301 tcacgtcgat cgcggacccg ggacggattt cgtgaagggc ctcgagcgcc ggcagcgcca
      361 tcacggcatc gcccacccag ttcggcatgc gaaggaggat gtcgctcacg gtccctccac
      421 gctccggaga aggcggcgca cctcatcggc cctggggccg gggggaagct ggtcgaggat
      481 ccgcttcagg cttcccgccg cgcctgatcg atcgtcgaga tggatctgcg cggcgtaaag
      541 tccccaccag gcctcgatca tcgacggcgc caggtcggcg gcgcgccggt acgaggcggc
      601 cgcctcgcgc cagagctcgc gcgattcgta aaggcgcccc aggttgtagt gcaggcgcgg
      661 ttcggcgggg gcgatcgcca gcgcccgctg gtaggtgttc agtgccgaga ggtattcccc
      721 ccgttcgccg cgcaggatcg ccaggttggt cagggccttc acgtcgccgg gggcgagcgt
      781 gaccgcctgc tcggcggccg gcagcgcgag gtcactccga ccgccgcgcg ccagcacgcc
      841 ggccagcgtc ctcatgaagt cggccatcgc cgcacggtcc ggcagcgtgt cgtagaactt
      901 cggatcgatc ccgcggaaat cggagagcgt gcctcccggt tcgcgccgga agatccggat
      961 gagcggcccc acccgcccac gctcgggccg gaattcggcc accagcgtca ggtgccgggc
     1021 cacgccgtcg tagaacgccg actgggccgg aaattcatcg atgcggctca ggtagcgatc
     1081 gcccagcgcg ctcgaaagga caatccattc gaacggcgcg tagtagggga ggctgtaggc
     1141 cgggtcgtaa cgatgcggct cgaccgagtc gaacgggatg aggaggagcg caaggctgtc
     1201 gctcatcgac tcgatcgagt agcgctcggc ggccaccagg ctccccttcg ggatggcgcc
     1261 gcgcgcccat tccgcggccg cgtcacgggt gctcgggcgc gcgcgccggg tcccctcctg
     1321 ccagctggtc acgagcgtgg gcccggcagc cagcgcgagt accaccagcc ccgtgaccaa
     1381 tcccatccgg tccgagctcc gaatcctcct cggcgcgagg ccgaggatcg tgccggccag
     1441 cacggccaac gggggaagaa ggaggaggaa gtagcgatcg aaggccacgc gccacgaacc
     1501 cagcagtgcc agcccggcca gggcgaagag tgcgacgagc cgccccgcgc ccccggccat
     1561 gagcgcggcg acaatcccgg ccagcccggc tccccacccc agccagccga gcgcgcgggg
     1621 ggccaggtca cgcaggtaga agagccaacc ggcctcgttg accgcccgga agtgacccgt
     1681 ggtcatgtgg aatcgttcgt acgtgacggc gcgccaggca tcgggaaggt tgagcacgat
     1741 ggcgggggac gtgacggcga acgcggccac ggacaccagg atgatccgca gcgccggacc
     1801 cgcccccccc gcaccgatca ccagcggaag gagcaggagt gcggcc
//
LOCUS       DASVOD010000308         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_439005, whole genome shotgun sequence.
ACCESSION   DASVOD010000308 DASVOD010000000
VERSION     DASVOD010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439005"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VF720_06880"
     CDS             <1..873
                     /locus_tag="VF720_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879116.1"
                     /translation="DSLIGRAAFYDLRRSEQPITFANFGAATPVSGEPAPLPPGIRQG
                     MTAGGLFQGTRYYFALKIGDERPNWSGLSNVVERRTNRIAGDAKLLVHVTTPVSKAPC
                     TNGRLADCMDVVDRGDLASVGGSGPRYFVYLLAAEFNELAGIQCGISYDSNLPDGDVN
                     QRGIDVFGWSLCANLEVPSTSGPQWPKPGSSNVITWNSTYACQLEATAVAGYFYMAAY
                     SPDSFQITPHSVDGLARVTNCAAVHTMVSPNHLGTARFSDGAALPGNNPCGVGQEIGI
                     QPTTWSRIKTLTAK"
     gene            1052..1834
                     /locus_tag="VF720_06885"
     CDS             1052..1834
                     /locus_tag="VF720_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7879117.1"
                     /translation="MGLGTIAGSAGALQVDDGGSGATPILFVHSYGGSRKHWSAQLAH
                     VRHDHRAAAMDLRGHGGSTPPSDNDWSIEALASDIGEVADGLGFDHFVLVGHSLGAAA
                     ASAYAGTHNDRVCGLMLVGAPGKVARPVARDIMTAMSADFDKVSSDYWEKLLQNAKPH
                     VREIVHEGMQAMGRDPGLKLIQAVFDDDPLVGLRRFHGPVLLISTPHGNSPSDLQNLM
                     PDLPNKMMVGTSHWVQMDKPDEFNLIMDSFLARVDQGSKVSV"
     gene            1938..>2092
                     /locus_tag="VF720_06890"
     CDS             1938..>2092
                     /locus_tag="VF720_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7879118.1"
                     /translation="MSSNDFLRRLNRATLDRQLLLERSKLSPLKAVERLVAMQAQVPR
                     PPFVGLWT"
BASE COUNT          387 a          689 c          634 g          382 t
ORIGIN      
        1 gacagcctca tcgggagagc cgccttctac gacctgcggc gctcggagca gccgatcaca
       61 ttcgccaact tcggagccgc cactccggtg tcgggggagc cggcgccact cccgccagga
      121 attcgccagg ggatgaccgc aggaggcctg ttccagggta cgcgctatta ctttgccctg
      181 aagatcggag acgaaaggcc gaactggtcc gggctatcga acgtcgtgga acgtcgaacg
      241 aaccgtatcg ccggtgatgc caagctcctg gttcacgtca ccacccccgt gtcgaaggct
      301 ccgtgtacca acggaagact tgccgactgc atggatgtcg tcgaccgggg ggatctggct
      361 tcggtcggcg gatcgggtcc gcgctatttc gtctacctgc tggccgcgga gttcaacgaa
      421 cttgcaggca tccaatgcgg tatctcctac gattcaaacc tcccggacgg tgatgtaaat
      481 cagcggggaa tcgatgtctt cggatggtca ctctgcgcca atctggaggt accgagcacg
      541 agtggccccc aatggccgaa gcccggaagc agtaacgtga tcacctggaa ctcgacttac
      601 gcctgccagc tagaggccac tgcggtcgcc ggttacttct acatggcggc ctattcgccc
      661 gattcattcc agatcacgcc tcactccgtc gacgggcttg ccagggtcac caattgtgcc
      721 gccgttcaca caatggtttc tcccaatcat ctcggcaccg cacgattcag cgacggagca
      781 gcccttcccg gcaacaatcc ctgtggcgtc gggcaggaga tcggcatcca acccacgacc
      841 tggagccgca tcaaaacgct caccgcaaag tagcccatcc cccggcacgc cccatgcatc
      901 cccgatcaaa cgtcgggcgg agtcgccgtc caggttcgtt ttaacggcgg ccgcatgtcg
      961 ggcaggtagg gaattccctg atgcccgacc cggagtgtca ttctgacccg cccgatgcgg
     1021 tgacactttt tcgcgtcgga gggattgcat catgggactc ggtacgatcg ccggatcggc
     1081 cggcgcactc caggtggatg acggcggctc gggcgcgacg ccgatcctgt tcgtccactc
     1141 gtacggcggt agtcgcaaac actggtcggc ccagctggcg cacgtgcggc acgaccaccg
     1201 ggcagcggca atggatcttc gcgggcatgg cggttccacg ccaccgtccg acaacgactg
     1261 gtcgatcgag gcccttgcct cggacatcgg cgaagtggcc gacgggctcg gcttcgatca
     1321 tttcgtgctt gtcgggcaca gcctcggcgc cgccgccgcc agtgcttacg ccggtactca
     1381 caatgaccgc gtctgcgggc tcatgctcgt cggcgcgccc gggaaggtgg cacgtccggt
     1441 ggcgcgcgac atcatgacgg cgatgtcggc cgatttcgac aaggtcagct cggactactg
     1501 ggagaaactt ctccagaacg ccaaacccca tgtacgcgaa atcgtgcacg aaggaatgca
     1561 ggccatgggt agggatcccg gcctcaagct catccaggcc gtcttcgacg acgacccgct
     1621 ggtgggcctg cgccgcttcc acggcccggt actcctcatt tccacgccgc acggcaattc
     1681 gccgagcgac ctgcagaacc tgatgcccga tcttcccaac aagatgatgg tcggcaccag
     1741 ccactgggtg cagatggaca agccggacga attcaacctg atcatggact cgtttctcgc
     1801 gcgggtcgac caggggtcga aagtttccgt gtaacgattg ctgtcggaga acgacgaacg
     1861 atcgtcgcgg gtccacctca ctgggagagg gggcccgcgt ttcgtttggg ctcgacgtat
     1921 cggctaacat ccgagggatg agctcgaacg acttcctccg ccgcctcaat cgcgccacgc
     1981 tggatcggca gctgttgctc gaacgctcga agctctcacc cctcaaggcc gtcgagcgcc
     2041 tggttgccat gcaggcccag gtgccgcggc cgccgttcgt cggactctgg ac
//
LOCUS       DASVOD010000309         3547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_126190, whole genome shotgun sequence.
ACCESSION   DASVOD010000309 DASVOD010000000
VERSION     DASVOD010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126190"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VF720_06895"
     CDS             complement(<1..644)
                     /locus_tag="VF720_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879119.1"
                     /translation="MRRRGGTVGHGNRSRVALAALLLIGGFLVPRPSGAGLISGLPGE
                     APADTGYVVPTAGQSLDTLDVDRNADRALVHPGDQVAGIAGGDQARTGVPGIVRPWLV
                     GGPGYGRLPLVVDGFPVGQGVVHWDNPAWFPPRAIDRFELTGGAGALVGDGSAGRALT
                     ANTFAGPARPVSVVSLLGGSFGHRLAELDFARQFGSVGFHADVADYGHDGFGPVG"
     gene            complement(652..1470)
                     /locus_tag="VF720_06900"
     CDS             complement(652..1470)
                     /locus_tag="VF720_06900"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7879120.1"
                     /translation="MARIDLHSHSLHSDGSHTPTELVAMARKAGITALALTDHDTVAG
                     LAELHAAAAEGGAALTVVDGVELSASAGMNDIHILGYFIDPRQAEFQIALKIFQEGRR
                     NRVITMLEKLKALGVELTLGDVERHAGGGSLGRPHVALALLDAGYIDTFDEAFARYLG
                     HRAPAFVGKPRFAPEEAIALIRAAGGVAVFAHPGTANRDDLIPRLKAAGLSGIEVWHP
                     KHGPGQRAHYLRVAKDFDLVPSGGSDFHGARVGDVVIGMSDVPESTLADLAARR"
     gene            complement(1549..>3547)
                     /locus_tag="VF720_06905"
     CDS             complement(1549..>3547)
                     /locus_tag="VF720_06905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879121.1"
                     /translation="SEQDRNPDCRRLRFHLDRTQPQTRRQRGSRGALDKVVTIKRKAQ
                     RFVQSGDLAKAIVEFDKLVESGDLEPYDYLQIGDILTRLRRLDDAVLRYRDAVAAYER
                     VGLYKNAIAICKKLLRVRPDAYDILRALGGLYALEGLHTDAIFYYLQYITAAPPDRET
                     PGIQDVGVRLLSMSLPSADVALKIVDVMHAAGCDVAGARPLFDLGIEFEEQGRKEWGQ
                     ALKGRAAQIVPNVDELEPSRRVASKEDGPIGPTDAMSTFAMSAEEAVFAPPLSIGPKP
                     PEAEPSEHADDEDDSVLTIDTEDGTFSGSGAAGGMAASSGSADPGIDFGMIDLGSPAP
                     ATNGNGAGSGAPSANEYLATPFAEEFVPGEPDDLCRQAEERLAEDDLAGALAAWLAAA
                     RSAFNFGQSRRAEEIYLQIVGKDPNHLEALQGLTEIAHINGERQKIVRFGCELGDVLL
                     ARELYVEAKLEFERVLQFDAGNEKAKSRVARLNSIEGVDKVTARPLAPMASEVQGAMI
                     TVRGEPVQTQSVNDLSEILSEFEKAMAAQVPTDDAQSHYDLGMAYIEMGMIEQAIESF
                     THASASETYQVRALEMVARCQIEAGRPEVAIESVDQALAVGVEEPGREAALMAMRGLA
                     LEQIGYPSEAAQEYQRALELDPGLAPAREGILRLGSSGAAA"
BASE COUNT          547 a         1214 c         1232 g          554 t
ORIGIN      
        1 ccgacaggcc cgaatccgtc gtggccgtaa tccgccacgt cggcgtgaaa tccgaccgac
       61 ccgaactgcc gcgcaaagtc gagctccgcc agccggtgac cgaagctccc gccgagcagc
      121 gagacgacgc tcaccgggcg cgccggcccc gcaaacgtgt tggcggtgag cgcacggccg
      181 gcactgccgt cgccgacaag cgcgccggcc ccgccggtga gttcgaatcg gtcgatcgcg
      241 cgcggcggaa accaggccgg gttgtcccag tgcacgaccc cctgcccgac cggaaagcca
      301 tcgaccacga gcggcagccg accgtaaccc ggcccaccga ccagccaggg gcgcacgatc
      361 cccggaacgc cggtcctggc ctggtcgcct cccgcgattc cggctacctg gtcgccggga
      421 tgaacgagtg cacggtcggc gttgcggtca acgtcgagcg tatcgaggga ttggcctgcc
      481 gtcgggacca cgtaaccggt gtcggcgggc gcctcacccg ggaggccgct gatcagcccg
      541 gcgccggagg gtcgtgggac gaggaatccg ccgatcagca acagcgccgc gagggccact
      601 cgggacctgt tgccgtggcc gacggtcccg ccgcgccgtc tcactacgga atcagcggcg
      661 cgcggcgagg tcggcaagcg tcgactcggg gacgtcggac atgccgatga ccacgtcccc
      721 cacccgcgcc ccatggaagt cggagccacc gctcggcacg agatcgaagt ccttcgcgac
      781 acgaagataa tgggcgcgct ggccgggacc atgcttggga tgccatacct cgataccgga
      841 gaggcccgcc gccttgaggc gcgggatcag gtcgtcgcga ttggccgtgc cggggtgcgc
      901 gaagaccgcc acgccgccgg ccgcccggat gagcgcgatg gcctcctccg gcgcaaatcg
      961 cggcttgccc acgaaggcgg gcgcgcggtg accgaggtag cgggcaaagg cctcgtcgaa
     1021 cgtgtcgatg taaccggcgt cgagcagcgc caacgccacg tgcggccggc cgagcgagcc
     1081 cccgccggcg tgccgttcga catcgcccaa tgtcagctcc acgccgagcg ccttcaattt
     1141 ttccagcatg gtgatcacgc ggttccgccg accttcctgg aaaatcttca gggcgatctg
     1201 gaactccgcc tgacgaggat cgatgaagta accgaggata tggatgtcgt tcatgccggc
     1261 cgacgcggag agctccacgc cgtcgacgac cgtcagtgcc gcccccccct ccgccgccgc
     1321 ggcgtgcaac tcggcgaggc cggccaccgt gtcgtggtcg gtaagggcga gggcggtgat
     1381 cccggccttt cgggccatcg ccaccagttc ggtgggcgtg tgcgagccgt cggagtgaag
     1441 cgagtgggaa tggagatcga tacgggccat gagcccggga tgatagaacg gcgtggtcgg
     1501 gttggctacg ctgcgcgttg gccaggctgc agtttggtca ggcgttcgtc aggcggcggc
     1561 gccgctcgac ccgagccgga ggataccctc gcgggccggc gccaggccag gatcgagctc
     1621 aagggcccgc tgatactcct gggcggcctc ggagggataa ccgatctgct cgagcgccag
     1681 gccccgcatg gccatcaggg ccgcctcgcg accgggctcc tcgacgccca ccgccagtgc
     1741 ctgatcgacc gactcgatgg ccacctcggg acgccccgcc tcgatctggc acctcgccac
     1801 catctcgagc gcccggacct ggtaggtctc cgaggccgag gcgtgggtga acgactcgat
     1861 ggcctgctcg atcattccca tttcgatgta ggccatgccg aggtcgtagt ggctctgtgc
     1921 gtcgtcggtc ggaacctggg ccgccatcgc cttctcgaat tccgacagga tctccgaaag
     1981 gtcgttgacc gactgcgtct gcaccggctc gccgcgcacg gtgatcatgg cgccctgcac
     2041 ctcgctggcc atgggggcca gcggacgggc ggtcaccttg tccacgccct cgatggagtt
     2101 gaggcgggcc acgcgcgact tggccttctc gttccccgca tcgaactgga ggacgcgctc
     2161 gaattcgagc ttggcctcca cgtagagctc gcgcgccagg agcacatcgc ccagttcgca
     2221 gccgaagcgg acgatcttct gccgctcacc gttgatgtgg gcgatctcgg tcagcccctg
     2281 cagggcttcg agatggttcg ggtccttgcc gacgatctga aggtagatct cctcggcgcg
     2341 gcgactctgg ccgaagttga aggcgctgcg cgccgcggcc agccaggcgg ccagggcacc
     2401 ggccaggtcg tcctcggcca ggcgctcttc ggcctggcga cagaggtcgt ccggctcgcc
     2461 gggaacgaat tcctcggcga agggcgtggc caggtactcg ttggcggatg gcgcgccgct
     2521 tccggccccg ttgccattgg tggcgggcgc gggggagcca aggtcgatca tcccgaagtc
     2581 gatgccggga tcggcggagc cgctgctcgc ggccatccca ccggcggccc ccgaaccgga
     2641 gaacgtcccg tcctcggtgt cgatggtcag aaccgagtcg tcttcgtcgt cggcgtgttc
     2701 cgagggctcg gcctcgggcg gcttcggccc gatcgacagg ggaggcgcga agacggcctc
     2761 ctcggccgac atcgcgaagg tggacatcgc gtcggtcggc ccgatcggtc cgtcttcctt
     2821 cgaggccacg cggcgcgagg gctcgagctc atcgacgttg ggcacgatct gggccgcacg
     2881 ccccttcagg gcctggcccc actccttccg tccctgctcc tcgaactcga tgccgagatc
     2941 gaacagcggc cgcgcgcccg ccacgtcgca gccggcggcg tgcatcacgt ccactatttt
     3001 cagcgccaca tcggcactgg ggagcgacat cgagagaagg cgaacgccca catcctggat
     3061 gccgggggtc tcccgatccg ggggggcggc cgtgatgtac tggaggtaat agaagatggc
     3121 gtcggtgtgg aggccctcga gtgcgtagag gcctcccagc gcccggagga tgtcgtaggc
     3181 gtccgggcga acccggagga gcttcttgca gatggcgatg gcgttcttgt agaggccgac
     3241 gcgttcgtag gcggctaccg cgtcacgata gcgcagcacc gcgtcgtcga ggcgacgaag
     3301 ccgggtcagg atgtcgccga tctggagata gtcgtacggc tccaggtcgc cgctctcgac
     3361 cagcttgtcg aactcgacga tggccttggc gagatcgccc gactggacga agcgttgcgc
     3421 cttccgcttg atggtgacga ccttgtccaa cgctccccga ctccctcgtt gtcgccgtgt
     3481 ttgcggctgt gtgcggtcca ggtggaagcg gagacggcgg cagtcggggt tccgatcctg
     3541 ctccgac
//
LOCUS       DASVOD010000310         6659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_698773, whole genome shotgun sequence.
ACCESSION   DASVOD010000310 DASVOD010000000
VERSION     DASVOD010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6659
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698773"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..4587
                     /locus_tag="VF720_06910"
     CDS             70..4587
                     /locus_tag="VF720_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEX7879122.1"
                     /translation="MFGLLFAAGESRAQTRPGTSISNTAYAAYSLAGAIDTVRSPSNV
                     VTIITKGGRTPAILEFLRHAPGAAGAEFVRVNTADSLLSLPLIPATTFQLGEVVYLRL
                     TDLDQNLDHGQPEIVAVELTLAPNGDQELFLLHETGPSTGIFTRAAASRAALNGTPGN
                     GRLDARNATEIHGRYVDRVDPTDVAVDAAVFVGEAPRLHLDKSVSNTRASIGDLVEYT
                     LRLDNPSGEDVPEAETARGVTIVDQLPPGFRYREGSARQGALVVDPAVSPDGSRLTFH
                     VGDMDPGDDVVFQYLVDVAAGARAGEAINTAEAATNTGAVSNTAQASILVLDDFFRRG
                     FIAGRVTVGACDSTVAGTGPGVFGVRLVLEDGTWTTTDENGRYHFEGVPAGMHVIAID
                     GESVPFELEPADCPDTHRFARSRDSQFVPLEEGTLWRADFRLTPRDPGMIHASPDSTL
                     EGEEAPDAPTTAEATDAPAGPPIVTDAFTETLAPGAAWVWPPERYGPSIPGLTVIVKH
                     DATVTPELWCNGRAVPKLNLSETRRNAAGTLAVTTWTGVDIQEGSNRLEARMLSADGG
                     AVVLVREVHYAGSPVDVVIVPSLSHLVADGLTPPVVAVRLVDRDGHPARERVVGSFHV
                     DPPYGSLLERDARHDNPLATLNAPQYQVGADGVALIELEPTPRTGEVVIRFPLDGHPR
                     EVRAWLEPTDRGWMLVGLADGTVAHRTVSDHMEAPTGDEDDYDTDGRIAFYARGRVKG
                     SWLLTLAVDSRGGNPGPSGDLDRVIDPDSYYTLYGDATTQDYDAPSSRKVYVRLERRE
                     FYALFGDTDAGLTRTELNRYTRRVNGFKTEYRGRNAGWTAFAAQSESRFVRREIPGNG
                     TSGLYDLQHPEMVVNSETIVLETRDRFRSEFILRSRTLHRHVDYDIDYAAGTLWFREP
                     VFSQDADFNPNWIVAVFESRDPESNRMEAGGRAAVYVDGDRFEAGATGVYDEDGTSPG
                     RLAGGDVRYRLDPVTTLRGEGAWSEDGDGREGNAWLAEIERRGSRVDGMAWYRRQDTG
                     FGLDQQNRGEQGIEKYGADVTWHATRADRARAQAFRQFNLASGARRDVGELALVHDAA
                     RIDLRAGIRHAVDRFAERADAVSDQLTAGGDWRVWNGRLSLKADHAQSIGGRNNNVDY
                     PTRTLLGLDWRMVRQVSLVASQEFTNGPTVDTRSARVGLVARPWTGGELATSIGRRTG
                     ESGERLFANLGLHQTWRVSERWFLDAGLDQSKTIGSYDSVRVNPGVPPASGGTADLLA
                     VSLGAARHGDAWQWNGRVEYLDAPGETRWSFVPSLFVQPRQAFGAALGGRIFLTDGPV
                     DRTRADIRASLALRPDNSPWIVANRLDWVVLDEQGPSTSNEEWRVIDNLHVNRRFGRA
                     FQATLQYGGKYIDRSLLGSSWNGYTDLWGAEARHDIAAHFDLGLSGGLRHSWTVGTSD
                     WYAGASAGWVMARGLWLQGGYNVTGFRDEDFSEAAVTVKGPFLRLSARFDTDALSRLG
                     FGDHAAGKDDR"
     gene            4584..6227
                     /locus_tag="VF720_06915"
     CDS             4584..6227
                     /locus_tag="VF720_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879123.1"
                     /translation="MTRRVVVLFTLALLVCGPIGRALAACLCSYGDGRFTTHTAITID
                     GAVLDWATVLLDTDNSSCDGSAPNVAPQPDRDAPVQSTGRDLNAFTYTWNNTYVQTLT
                     GRAASDANVQRFIYYADTDNDGKMETGERCIVATWKGSNRLVELFLGRYLQVAAGGDP
                     MVDINGYGDGYDLPGTITGLPPAGQPHYSGNWGTLSGVQMEWAVPWADAPGAPGLGIP
                     AGQAFTFHVSSTNSNPAAGSFPAQVDDNMAGCGGGAATSQYAGLDFLFDRVITASAST
                     TVYGAHRIINQGNGIDRFDLTAGTPTGGHTPTMAFYKDVDKSGTFTGGDVALTDTGGG
                     LSVDTGAMAAGDTIWVLIRYVVASNAAGTATIISTARSAFDSHWTDIVTDQVVVSPLP
                     NLVMTKTRSTVYDPVVGAAFNAKAIPAARVDYTIRVENRGLGTVDIGTVIVTDAIPAR
                     TRLYVGNLNGVGSGPLVVTDGAPSSGLSYLFSSLSSTTDNVSFSSDNGVSYTYTPVPD
                     ADGFDGAVTNLRVTFTGIMNATGITGHPYVTMRFRVEVR"
     gene            complement(6249..>6659)
                     /locus_tag="VF720_06920"
     CDS             complement(6249..>6659)
                     /locus_tag="VF720_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX7879124.1"
                     /translation="VGDAVITTGGELTALHVIHAVGPRWGEGNEDEKLRSTIYRTLRV
                     ADQNGLRSLAMPAISTGEYGFPPRRAAEIILDTTIRYLNVQSTLTRVAIVLSDATMRD
                     LFVEVAEGMEAAGVLKTRPIPPGELGLVETPAGN"
BASE COUNT         1045 a         2391 c         2198 g         1025 t
ORIGIN      
        1 ccaccgcgtc gtccttcccg gagagtgccg ctgatgggat ggttccgtac gcgtctttgg
       61 atggtcgcga tgttcggcct gctgttcgca gcgggcgaat cgcgcgcaca gacgcgtccc
      121 ggcacgtcga tctccaacac cgcctacgcc gcctacagcc tggcgggtgc gatcgacacc
      181 gtccgctccc cgtcgaacgt cgtcacgatc atcacgaagg gtgggcgcac acccgccatc
      241 ctcgagttcc tgcgccacgc gcccggtgcc gccggggctg aattcgtgcg cgtgaacacg
      301 gccgattcgc tgctgtcgct gccgctgatt cccgccacca cgttccagct gggcgaagtg
      361 gtctacctcc gcctgaccga cctggaccag aacctcgatc acggccagcc ggagatcgtg
      421 gcggtggaac tcacgcttgc gccgaacggc gatcaggaac tcttcctcct gcacgaaacc
      481 ggcccgtcca ccggcatctt cacgagggcg gccgccagcc gcgcggccct gaacggcacg
      541 ccgggcaacg ggcgcctgga cgcgcgcaac gccaccgaga tccacggccg ctacgtcgac
      601 cgggtcgacc cgaccgacgt ggccgtcgac gcggcggtct tcgtcggcga ggcgccgcgc
      661 cttcacctcg acaagtcggt gtcgaacacc cgggcctcga tcggcgacct cgtggaatac
      721 accctccgcc tggacaaccc gtcgggcgag gacgttccgg aggccgaaac cgcccggggt
      781 gtcaccatcg tggaccagct gccgcccggc ttccgctatc gcgagggatc ggcccggcag
      841 ggggctctcg tcgtcgatcc ggccgtgagc cccgacggca gccgactcac gttccacgtg
      901 ggcgacatgg atcccggcga cgacgtcgtg ttccagtacc tcgtggatgt cgccgcgggc
      961 gcccgggcgg gggaggccat caacacggcc gaggccgcca cgaacacggg tgccgtctcc
     1021 aacacggccc aggcctcgat cctggttctc gacgacttct tccgtcgcgg cttcattgcc
     1081 ggccgggtca cggtcggcgc gtgtgactcc acggttgccg gaaccgggcc cggcgtcttc
     1141 ggggtacgtc tcgtcctcga ggacggcacc tggacgacca ccgacgagaa cggccgctat
     1201 cacttcgagg gcgttcccgc cggcatgcat gtcatcgcca ttgacggcga gagcgttccg
     1261 ttcgagctcg agccggccga ttgccccgac acgcaccgct ttgcccggag ccgggactcg
     1321 cagttcgttc cgctcgagga gggaacgctc tggcgcgccg acttccggct gacgccgcgc
     1381 gaccccggga tgatccacgc gtcgcccgat tcgacgctcg aaggggagga ggcgccggac
     1441 gcgccgacga cagccgaggc caccgatgcg ccggccggtc cccccatcgt gaccgacgcg
     1501 ttcaccgaaa cgctcgcccc gggcgcggcc tgggtgtggc cgcccgagcg gtatggcccg
     1561 tcgatccccg gcctcaccgt catcgtgaag cacgacgcca cggtcacacc cgaactctgg
     1621 tgcaacggcc gcgcggttcc gaagctcaac ctctccgaaa cgcgccgcaa cgccgccggc
     1681 acgctggcgg tcacgacctg gacgggcgtc gacatccagg aaggatcgaa ccgcctcgaa
     1741 gcacgcatgc tgtcggccga tgggggtgct gtcgtcctgg tccgcgaagt ccactatgcc
     1801 ggttcgccgg tcgatgtcgt catcgttccg tcgctgtcgc acctggtggc cgacggcctc
     1861 acgccaccgg tcgtggcggt gcgtctcgtc gatcgcgacg gccacccggc gcgagagcgc
     1921 gtggtcggtt cgttccatgt cgatccgccg tacggctcgc tcctggaacg cgatgcccgg
     1981 cacgacaacc cgctggccac cctcaacgcg ccgcagtacc aggtgggcgc ggacggcgtg
     2041 gccctgatcg aactcgaacc gacgccgcga accggcgaag tggtgatccg ctttccgctc
     2101 gacggccacc cccgcgaggt gcgcgcctgg ctcgagccga ccgaccgcgg ctggatgctc
     2161 gtgggcctcg ccgacggaac ggtggcccat cgcacggttt ccgatcatat ggaagcgccg
     2221 accggcgacg aggacgatta cgataccgac ggacgcatcg ccttctacgc gcgaggccgg
     2281 gtgaagggat cgtggctgct gacgctggcg gtggatagca ggggtggcaa ccccggtccg
     2341 tcgggcgatc tcgaccgcgt catcgacccg gacagctact acaccctcta cggcgatgcc
     2401 accacgcagg attacgacgc cccgtcatcg cgcaaggtgt acgtacgact cgagcgccgc
     2461 gagttctacg cgctcttcgg cgacacggac gccggcctta cccgcaccga gctcaaccgc
     2521 tacacgcgtc gggtgaacgg cttcaagacg gagtaccgcg gccggaacgc cggttggacg
     2581 gccttcgccg cccagtcgga gagccgcttc gtccgtcgcg agatccccgg caacggcacg
     2641 tcgggcctgt acgacctcca gcaccccgaa atggtggtca actcggaaac catcgtcctc
     2701 gaaacccgcg accgcttccg gagcgagttc atcctccgct cgcgcacgct gcaccgccac
     2761 gtggattacg acatcgacta tgcggccggc accctctggt tccgcgagcc ggtcttcagc
     2821 caggacgccg acttcaaccc gaactggatc gtggccgtct tcgaatcgcg cgatccggag
     2881 tcgaaccgca tggaggcggg cggccgcgcg gcggtctacg tcgatggcga ccgtttcgaa
     2941 gcgggcgcca ccggcgtgta cgacgaggat ggcacgagcc ccggccgact ggccggcggc
     3001 gatgttcgct accgtctcga tccggtcacc acgctccgcg gcgaaggcgc ctggagcgag
     3061 gatggcgacg gccgcgaggg aaatgcctgg ctcgccgaga tcgaacggcg cggtagccgg
     3121 gtcgacggca tggcctggta ccgccgccag gacaccggtt tcggcctcga ccagcagaac
     3181 cggggcgagc agggcatcga gaagtacggc gccgacgtca cctggcacgc cacgcgcgcc
     3241 gaccgggcac gcgcgcaggc gttccgccag ttcaacctgg cctccggcgc ccgacgcgac
     3301 gtcggcgagc tggccctcgt gcacgacgcc gccaggatcg acctccgcgc cggcatccgc
     3361 cacgcggttg atcgattcgc cgagcgcgcc gacgccgttt cggaccagct caccgccggc
     3421 ggcgactggc gggtctggaa tggacgactg agcctgaagg ccgaccacgc gcagtcgatc
     3481 ggcggcagga acaacaacgt cgattatccg acgcggacgc tccttgggct cgactggcgg
     3541 atggttcgcc aggtgagcct ggtggccagc caggaattca ccaacggccc gacggtcgac
     3601 acgcgctcgg cgcgggtggg cctcgtggcc cgcccgtgga cggggggcga actcgccacc
     3661 tccatcggcc gccgcaccgg cgaatcgggc gagcgcctct tcgccaacct cggccttcac
     3721 cagacatggc gcgtttcgga acgatggttc ctcgacgccg ggctcgacca gtcgaagacg
     3781 atcggatcgt acgactcggt ccgggtgaac ccgggggtgc cgccggcttc cggtggcacc
     3841 gccgacctcc tggcggtttc gctgggtgcc gcccgtcacg gcgatgcctg gcagtggaat
     3901 ggtcgcgtcg agtatctcga tgcgccgggc gagacgcgct ggtcgttcgt ccccagcctc
     3961 ttcgtccagc cgcgccaggc tttcggcgcc gcgctgggcg gacgtatctt cctcaccgac
     4021 ggtccggtcg atcggacgcg cgccgacatc cgggccagcc tggccctgcg cccggacaac
     4081 tcgccctgga tcgtggccaa ccgtctcgac tgggtcgtgc tcgacgaaca gggaccgtcc
     4141 acgagcaacg aggaatggcg cgtcatcgac aatcttcacg tgaatcgccg gttcggtcga
     4201 gccttccagg ccacgctcca gtacggcggc aagtacatcg accgctcgtt gctgggttcc
     4261 tcatggaacg gctacaccga cctgtggggc gccgaggcgc ggcacgatat cgccgcccat
     4321 tttgatcttg gactgtcggg cggccttcgc catagctgga cggtcggtac gtccgattgg
     4381 tacgcgggcg cctccgccgg ctgggtgatg gccagggggc tctggttgca ggggggatac
     4441 aacgtgaccg gattccggga cgaggatttc agcgaggcgg ccgtcacggt gaaggggccc
     4501 ttcctccgcc tcagcgcccg cttcgatacc gacgcgctct cgcgcctcgg cttcggcgac
     4561 cacgccgccg ggaaggacga tcgatgactc gtcgtgtcgt cgtcctgttc accctggcac
     4621 tcctcgtgtg cgggccgatc ggccgcgcgc tggcggcctg tctttgctcg tatggcgacg
     4681 gccgtttcac gacccacacc gccatcacga tcgacggcgc cgtcctcgac tgggccaccg
     4741 tgctgctcga cacggacaat tcctcctgcg acggctcggc gcccaacgtg gcaccgcagc
     4801 ccgaccggga tgcgcccgtt cagtccaccg ggcgcgacct gaacgccttc acctacacct
     4861 ggaacaacac ctacgtgcag acgctcaccg gccgcgccgc cagcgacgcc aacgtgcagc
     4921 gcttcatcta ctacgccgac accgacaacg acgggaagat ggaaaccggc gagcggtgca
     4981 tcgtggccac ctggaaggga tccaaccgac tggtcgagct cttccttggg cgctacctgc
     5041 aggtggccgc cgggggcgac ccgatggtcg acatcaatgg ttacggagac ggctacgacc
     5101 tccccggcac catcaccggc ctccccccgg ccggacagcc tcactattcc ggcaactggg
     5161 gcacgctcag cggcgtgcag atggagtggg cggttccctg ggccgacgcg ccgggggcgc
     5221 ccggtctcgg catccccgcc ggccaggcct tcacgttcca cgtgtcgagc accaactcga
     5281 acccggcggc cggttcgttt ccggcgcagg tcgacgacaa catggccggc tgcggcggcg
     5341 gggcggccac gagccagtac gccggcctcg acttcctgtt cgatcgcgtc atcacggcat
     5401 cggcaagcac gaccgtgtac ggcgcgcacc gcatcatcaa ccaggggaac ggaatcgacc
     5461 ggttcgacct cacggccggc acgcccaccg gcgggcacac gccgacgatg gccttctaca
     5521 aggacgtgga caagagcggc acgtttaccg gcggggacgt ggcactcacc gataccggtg
     5581 gcgggctgtc ggtcgatacc ggcgccatgg ccgccggtga cacgatatgg gtgctgatcc
     5641 gctacgtcgt ggcctccaac gccgccggca ccgccacgat catctccacg gcgcgctctg
     5701 ccttcgacag tcactggacc gacatcgtca ccgaccaggt cgtcgtgtcg ccgctcccga
     5761 atctggtgat gaccaagacg cgttcgaccg tctacgaccc cgttgtgggc gcggccttca
     5821 acgccaaggc catcccggcc gcgcgcgtcg actacacgat tcgcgtcgag aatcgcgggc
     5881 tcggcaccgt cgacatcgga accgtgatcg tgaccgatgc cattcccgca cggacgcgcc
     5941 tctacgtggg caacctgaac ggggtcgggt cggggccgtt ggtcgtcacc gacggagcgc
     6001 cgtcgagcgg gctgtcgtac ctgttcagca gcctctcatc gaccacggac aacgtatcgt
     6061 tctcgagcga caatggcgtc tcgtacacct acacgccggt gcccgacgcc gatggattcg
     6121 acggcgcggt gaccaacctg cgcgtgacat tcacgggaat catgaacgcc acgggtatca
     6181 ccgggcatcc gtacgtgacg atgcggttcc gggtggaggt tcgctagcgg gcagaaggcg
     6241 tgcgccggtc agttgccggc gggagtttcc acgaggccca gttcacccgg cggaatcgga
     6301 cgggtcttga gcacgccggc cgcttccatc ccctcggcca cctccacgaa caggtcgcgc
     6361 atcgtggcgt ccgacaagac gatcgccacg cgcgtcagcg tcgattgcac gttcaggtag
     6421 cggatcgtgg tgtcgaggat gatttccgcg gcgcgccgcg gcgggaagcc gtattcgccg
     6481 gtggagatgg ccggcatcgc caggctgcgc aggccattct ggtcggccac gcgaagcgtg
     6541 cggtaaatcg tggagcggag cttctcgtct tcgttcccct cgccccagcg cggccccacg
     6601 gcatggatca cgtggagggc cgtcaactcg ccgccggtcg tgatgaccgc gtcgccgac
//
LOCUS       DASVOD010000311         8764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_908300, whole genome shotgun sequence.
ACCESSION   DASVOD010000311 DASVOD010000000
VERSION     DASVOD010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8764
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908300"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VF720_06925"
     CDS             <1..1361
                     /locus_tag="VF720_06925"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX7879125.1"
                     /translation="IVVSNCSESRVRDLARGLEQFFAVVDRSWGGTVPVGQADGKRRI
                     YIFASHAEFQSILSMSPRYGRRSKVRGLFLRDGDDVTLLVDAGNTPWPLGTLQHELVH
                     DLLAARSMHVPLWFNEGLAEYYSNFLTGPKEVQIGRPLVDHLEYMQQAPWLDMDELFQ
                     VDHDSPHYNEGSKAGTFYAQSYALVHYLLVGRPGMTPSWRPYVEALDRGQDQAKAFEE
                     AFALDQRGLNEELAAYVARDSWPSKRIKISDLKLPKERKPISLNPTRALVRIGDLVHR
                     MDEDDGNVQETRAWYEEALRRTPGDAGAMAALAALAEYKAPERPSFPPDQPEPSVNDP
                     NKKPRYLSDGAVADAFDAINSMIQSQQLEEAAEAIEALIPRCPNPRDQDTLRLKLSSL
                     KPTIAHNKRVDAYNAAVRALNEGRRTDARRILDGITGPGTDKELEAAIRKLRATVEGL
                     EP"
     gene            complement(1419..1682)
                     /locus_tag="VF720_06930"
     CDS             complement(1419..1682)
                     /locus_tag="VF720_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6289 family protein"
                     /protein_id="HEX7879126.1"
                     /translation="MNRKFAGRKLIVATLTLAWMIGGAVIALRTTAVEAAPGANCTYY
                     SSANKTTVVGMYGKDCCNNTVAWGQKTKWSTCGGCFVCTPPPR"
     gene            complement(1788..3677)
                     /locus_tag="VF720_06935"
     CDS             complement(1788..3677)
                     /locus_tag="VF720_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879127.1"
                     /translation="MTNPPAALTRILIALSFLLAALLACRQLASADLGFHLKTGDYIL
                     GGHGWPKTDPFTVTMKGHPYIDTSWGFDVLVTIANKLAGPTGIGILAIFLTLATLTLI
                     GARTIDKTQDGLALGLGLLAAAIAMEMRYEPRPETLSYAFLALQCFLLERRAAGRPTP
                     IWSFVIVQWAWTNTHSLFVIGWLAIAIFLVGGWLERRKIDRQLALGLLASMAVAFLNP
                     YGLEGVKFPFTLLTRFQQGNPFAESIGEFISPLSLKLSDQFPFYPVWPIWTFRVLVVL
                     AALAILPLARHRRFTAILLMLAMFPLAAKMIRNIPLFVVVAVPAIIATFPLGQWLTKR
                     RPVFRTAVPVAGILVAIILCLRVYHDAYYLDTRRPDRFGFGWNRFALPVETAAYLNSH
                     DMPGAMLNHLNFGGYLMHATKGPVFIDGRLEVVGESFYNDYLRIFGSEVALEQAVQRN
                     GIGYIVTPYTIAPELIRRVSADPRWTLTHLDNVAAVFVRNDRAGTKDRMSSFVDSKLA
                     SQLAAGPPSVSWNQLPGMGGPPRAGKLTRWVGGLSGRQTFPNDEFYVGLFCYLRKEYE
                     AAGRHFTEAIRTSRGDWYDPYANLGAVLWRNNMKPAAEAAYRVVLIEDPDNSVARQRS
                     TGTER"
     gene            complement(3674..4285)
                     /locus_tag="VF720_06940"
     CDS             complement(3674..4285)
                     /locus_tag="VF720_06940"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX7879128.1"
                     /translation="MEKIGAEKRDETTAVILCGGNSRRFGRDKAAADHHGQSLLDHVL
                     EVCGVHADQIILACGRESRYASLGLTLALDAPGSDGPLAGIDAGLKAASHPSILIVAV
                     DLAGVNPRGIEMLREAWREDPTADLVLPRTERGVEPLLCVGRREPLRAAVATLIATSD
                     PAPRRLPEFLKTRFIDVATGPGDPLHRALTNINTPQDLAALDG"
     gene            4706..5500
                     /locus_tag="VF720_06945"
     CDS             4706..5500
                     /locus_tag="VF720_06945"
                     /inference="COORDINATES: protein motif:HMM:NF021469.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin"
                     /protein_id="HEX7879129.1"
                     /translation="MVLAPRVPPPVAAALIEKDIDYVDLAGNCHLAPTPDQLVHVEKR
                     APERIRGTRNIRPQGYQVVFALLARPELIAEPVREIAMQAGVGKTVVGQTLERLEEEG
                     FIGGNGRKRKLINRARLLTKWLTGYADVLRPRWILGRFRTAKEGKEELPDRVEDYFKG
                     QTEDRPWGFGGTVAADNLTQHYRGFEIVVHTEELPADFARTLRALAAPTGDLIVMRTP
                     GPLAYKGPLPHVVHPLLVYTELMAGDDERAWETARMVLDQYLPELK"
     gene            5497..5856
                     /locus_tag="VF720_06950"
     CDS             5497..5856
                     /locus_tag="VF720_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879130.1"
                     /translation="MTPFVAEQIQAVDDLHVACGETPLVLVGAMALQWHFQKWEATRD
                     MDMSIAASVNEVSNITGRLRGWKQDLHQGHAWQSPAGVRVDILPAGPEILEAGEIVWP
                     RTDRRSTRKSRQSLKTF"
     gene            complement(6016..6516)
                     /locus_tag="VF720_06955"
     CDS             complement(6016..6516)
                     /locus_tag="VF720_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007944558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7879131.1"
                     /translation="MSTFKRFTTCLWFDHQAEEAAKFYTSIFRNSRITDVFRYSEVGQ
                     EVHGRPPGSVMVANFDLDGQSFMALNGGPAFKFNEAVSIIINCDTQDEVDYYWEKLGA
                     GGDPRAQQCGWLKDKYGLSWQVTPNGMEEMLRDENSPGAKRAFAAMMEMKKLDIGELE
                     KAFRGE"
     gene            6635..>8764
                     /locus_tag="VF720_06960"
     CDS             6635..>8764
                     /locus_tag="VF720_06960"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX7879132.1"
                     /translation="MSDDAPAVPRLDGHDKVTGRAQYVDDIHMPGMLHGATIRSTIAR
                     GKITGITFDPALDWSGFTIVTAADIPGKNEVLLLTTDQPFLADGHVRHHAEPILLLAH
                     EDREMLRRAARAITISYAEEPACLDVRQALRPEYLQHGRDNTFHSIDIVKGDVDTGFE
                     SADLVLDETYETGAQEQAYIEPQGMIARPAPEGSGIEIFGSLQCPYYVVKAVTHLLGV
                     PAEELRVVQTTTGGGFGGKEEYPSIIAGHAALLALKSGRPVKLVYDRYEDILATTKRH
                     PSITRVKAGATRDGRLTALEIDLVLDGGAYVTLTPVVLSRASLHASGPYEVPNITIRA
                     RARLTNTPPHGAFRGFGAPQSIFAVERHIDRLARSLGIAPLEFRRRNYLKLESVMATG
                     QVVRDDIDMHALANEVYERLGGAAREAELAKANAQSTHTRSALGFAAFYHGAGFTGSG
                     EERLASQVAARLDVDGRVEILSSNTEIGQGTVTIFSIIAAKALGLEPSEIRVAPPDTS
                     RVPDSGPTVASRTAMVVGWLVQQAVLDLTVKLAEKCSDSLLVVAPESLRGDAFRRAAA
                     QAVESGCRIVGRSRYEKPSFVTWDEVNYRGDAYATYAWAVYGCEVEVDTRTFEVSVKR
                     FVANQDIGGVLHKTLADGQIQGGVVQGLGWALCEQVVYDKGAVRNAALSTYVIPTFVD
                     VPPIEVLFATTPYEFGPMGAKGIGELPM"
BASE COUNT         1622 a         2732 c         2901 g         1509 t
ORIGIN      
        1 atatagttgt gagcaactgc tccgagtcgc gtgtgcgcga cctggcccgg ggtctcgagc
       61 agttttttgc ggtggtcgat cggagttggg gcggaacggt tccggtcggc caggctgacg
      121 gcaaacggcg gatctacatc ttcgccagtc atgccgagtt ccaatcgatc ctgtcgatgt
      181 ccccccgtta cgggcgccgg tcgaaggtgc gcggcctctt cctgcgggat ggcgacgacg
      241 tcaccctcct ggttgatgcc ggcaacacgc cctggccgct gggcaccctg cagcacgaac
      301 tcgtccacga cctcctggcc gctcgctcga tgcatgtgcc gctctggttc aacgaaggac
      361 tggccgagta ctacagcaac ttcctgacgg gtccgaagga agttcagatc ggccgaccgc
      421 tcgttgacca cctggaatac atgcagcagg ccccctggct cgacatggac gaactcttcc
      481 aggtggatca cgactcgccg cattacaacg agggaagcaa ggcggggacc ttctacgccc
      541 agtcgtacgc gctcgttcat tacctgctgg tgggccgacc tggcatgacg ccgagctggc
      601 ggccatacgt cgaagccctg gaccgcggcc aggatcaagc aaaggctttc gaggaggcct
      661 tcgcgctgga ccagcgggga ttgaacgagg agctggccgc ctacgtagcc cgggactcct
      721 ggccatcgaa gcgcatcaag atctcggatc tcaagctccc caaggagcgc aagcccattt
      781 cactcaaccc gacccgggcg ctcgtgcgca tcggcgacct cgtgcaccga atggacgagg
      841 acgacggcaa cgtccaggag actcgggcct ggtacgagga ggcgttgcgc cgcactcccg
      901 gcgatgccgg tgccatggcg gcgcttgcgg cgttggccga gtacaaggcg ccggagcggc
      961 ccagcttccc gcccgaccag cccgagccgt ccgtcaacga tcccaacaag aagccgcgct
     1021 acctctccga cggggccgtt gccgatgcgt tcgacgcgat caactccatg atccagagcc
     1081 agcagctcga ggaggccgcg gaagccatcg aggccctgat cccccgctgc ccgaacccgc
     1141 gcgaccagga cacgctccga ctcaagctgt cgtcgctcaa gcccacgatc gcccacaaca
     1201 agcgtgttga tgcctacaac gccgccgtcc gcgccctcaa tgaaggacgc cggaccgacg
     1261 cccgccggat cctcgacggc atcaccggcc ccggcaccga caaggaactc gaagccgcga
     1321 tccggaaact ccgcgccacc gtggaggggc tggagccctg aaatcagaag ggacgggatt
     1381 gctcccgccc cttccgttca aactgtcggt tcagcccctc agcgtggcgg cggcgtacag
     1441 acgaaacatc caccgcaggt ggaccacttc gtcttctgtc cccaggccac ggtgttgttg
     1501 cagcagtcct tgccatacat gccgacgacc gtcgtcttgt tcgcgctgct gtaatacgtg
     1561 cagttggcgc cgggcgccgc ttccaccgca gtcgttcgaa gcgcaatcac tgccccgccg
     1621 atcatccatg ccaacgtcag ggtagcgaca atcagtttgc ggccggcaaa cttcctgttc
     1681 attgcgtcct ccttgtggaa tacgggcgcg ggggtgcgcc cgggggaagc ggaggctagc
     1741 acgggaatgg cctgagatga actattcgtt cgaggcatga atggaaatca gcgctccgtg
     1801 ccggttgacc gctgacgtgc cacgctattg tccggatcct cgatcaggac tacccggtat
     1861 gccgcctcgg cggccggctt catgttattt cgccacagaa ccgcgcccag gttcgcgtac
     1921 gggtcgtacc agtcaccccg actggtgcga atcgcctcgg tgaagtgccg ccccgccgcc
     1981 tcgtactcct tccggaggta gcagaacagc cccacgtaaa actcatcatt cggaaacgtc
     2041 tggcggccgg acaggccgcc aacccatcgc gtgagcttgc cggcgcgagg agggccaccc
     2101 atgccgggca actggttcca cgaaactgat ggcgggccgg cggcaagttg gctggccagc
     2161 tttgaatcga cgaaggacga cattcgatcc tttgtacccg cccgatcatt ccgcacaaac
     2221 accgccgcca cgttgtcgag gtgcgtcagt gtccaccgtg gatccgcgct cacccgccgg
     2281 atgagctccg gcgcgatggt gtatggcgtc acgatgtatc cgatgccgtt gcgctgcacc
     2341 gcctgctcca gcgccacctc ggaaccgaag atgcgcaggt agtcgttgta gaacgactcc
     2401 cccaccacct cgagtcgccc gtcgatgaag accggcccct tcgtggcgtg catcaggtag
     2461 ccgccgaagt tcaggtggtt gagcatcgcg ccgggcatgt cgtggctgtt gagatatgcc
     2521 gcggtctcga ccgggagcgc gaatcgattc cagccgaagc cgaagcggtc ggggcggcgg
     2581 gtgtcgaggt agtaggcgtc gtgataaacg cgcaggcaga ggatgatcgc aaccaggatg
     2641 ccggcgacgg ggacggcggt gcggaacacc ggccggcgtt tcgtgagcca ctggccgagc
     2701 gggaatgtcg cgatgatcgc gggcacggcc acgaccacga agagcgggat gttgcggatc
     2761 atcttcgcgg ccagggggaa catggccagc atcaacagaa ttgcggtgaa tcggcggtga
     2821 cgggccaggg ggaggatggc cagcgcggcg agaaccacca gtacgcggaa cgtccagatg
     2881 ggccagacgg ggtagaacgg aaactggtcg gagagtttga gtgacagcgg cgagatgaat
     2941 tcgccgatgg attcggcgaa ggggttccct tgctggaatc gtgtgaggag cgtgaaaggg
     3001 aatttcacgc cctcgaggcc atacgggttg aggaacgcca ccgccatcga ggccaggagc
     3061 cccagggcga gttgcctgtc gatcttgcgg cgctcgagcc acccgcccac caggaagatg
     3121 gctatggcga gccatccgat cacgaacagg ctgtgggtgt tcgtccacgc ccattggacg
     3181 atgacgaagg accagatcgg tgtcgggcgg cccgccgccc gacgttccag caggaaacat
     3241 tgcagcgcga ggaaggcgta tgaaagcgtt tcgggcctcg gctcgtagcg catttccatg
     3301 gcgatggcgg cggcgagaag gccaaggccg agcgccagcc cgtcctgtgt cttgtcgatc
     3361 gtgcgggcgc cgatgagtgt gagcgtggcc agcgtgagga atatcgcgag gattccgatg
     3421 ccggtggggc cggcgagctt gttggcaatc gtcaccagga cgtcgaagcc ccagctggtg
     3481 tcgatgtagg ggtggccctt catcgtgacg gtgaagggat cggttttcgg ccagccgtgg
     3541 ccgcccagga tgtagtcgcc ggtcttcagg tgaaaaccga ggtcggcgct ggcgagctgg
     3601 cggcaggcga gcagcgcggc caggaggaac gacagggcga tgaggatgcg agtgagcgcg
     3661 gccggggggt tcgtcatccg tcgagggcgg cgaggtcttg gggggtgttg atgttggtga
     3721 gggcgcggtg gagcgggtcg ccgggtccgg tggcgacgtc gatgaaccgt gttttcagga
     3781 attccggcag gcggcgcggg gcggggtcgc ttgtcgcgat gagagtggct accgcggcgc
     3841 gtagcggctc gcgtcggccc acacaaagga gtggctcgac gccgcgctcg gtccgcggaa
     3901 gcaccaggtc ggcggtcgga tcctcccgcc aggcctctct cagcatctcg atgccacgcg
     3961 ggttcactcc ggcgaggtcg acagccacga tgagaatact cgggtggctt gcggctttga
     4021 gccccgcgtc gatgccggcc agcgggccat ccgagcccgg cgcatccagt gccagcgtga
     4081 ggcccaggga ggcgtagcgg ctctcgcgac cgcaggccag gatgatctgg tcggcgtgaa
     4141 ccccgcacac ctcgagcacg tgatcgagca gggattgacc atggtggtcg gccgctgcct
     4201 tgtcgcgacc gaatcgacga ctgtttcccc cgcagaggat gacggcggtg gtttcgtctc
     4261 ttttctccgc gccgatcttt tccattttcg gagattacct tttgtccccg atatttctga
     4321 aatcgccgct tctctcatta atttccgaat ttcgatttac tgttcttgtt ttttgtgatt
     4381 tatttaaatt attgaaaata aatgatttat gaaatatttg tcctcttttt ttcgcttgtc
     4441 cccttaaaga ggacagtgta ttatcgcgga catgaaagag ccgacgcaag gcgctattga
     4501 gccgtatcta gttaaaatac aaggtcttaa cacagccatc ggggccaggg ccgtgcgtgg
     4561 ccggactggc gaatggcggt tgcacgtcaa gacccgaggt ggacaccgga caacctatgt
     4621 cgttgagcta ctcgaagttc agcccacaac agcattggcc gtccggctgg cggagaggca
     4681 ggccgcaaac cacgacgttc gatggatggt gctggccccc cgcgtgccac cgccggtggc
     4741 cgcagccctg atcgagaagg acatcgacta tgtggacctg gcgggcaatt gccacctggc
     4801 ccccactcct gaccagttgg tgcacgtgga aaagagggca ccggagagaa ttcgcggaac
     4861 gcgaaacatt cgaccacagg ggtaccaggt ggtgttcgca ctattggccc gacccgaact
     4921 catcgccgaa ccggtgcgag aaatcgccat gcaggcgggt gtcggcaaga cagtggtggg
     4981 gcagacactg gaacgtcttg aagaagaagg cttcatcgga gggaacggcc gaaagagaaa
     5041 attgataaat cgtgctcgac tgctgacgaa atggctgacc ggctacgccg acgtcctgcg
     5101 gccacggtgg attcttggga ggttccgcac ggccaaggaa ggcaaggagg aactgcccga
     5161 ccgcgtcgag gactacttca aggggcagac cgaggaccgc ccatggggat tcgggggtac
     5221 agtagctgcg gacaacctga cgcagcacta caggggcttt gagatagtgg tgcacacgga
     5281 agaattgccg gctgacttcg ccaggacgct gcgagcactg gcagcaccga ccggtgacct
     5341 gatcgtgatg cgcacgccgg gccccctcgc gtacaaggga cccttgccgc atgtcgtcca
     5401 tcccctgctg gtgtacaccg agctcatggc cggcgacgat gagcgcgcat gggaaacggc
     5461 gcgcatggtg ctcgatcaat acctgccgga actcaaatga ccccattcgt ggccgagcag
     5521 atccaggcag tcgacgatct ccatgtcgcc tgcggagaga cacctctcgt actcgtcggc
     5581 gccatggccc tgcagtggca ctttcagaaa tgggaagcga cacgggacat ggacatgtcg
     5641 atcgccgcat cggtcaacga ggtgtccaac atcactggac ggctgcgtgg ctggaagcag
     5701 gatctccatc agggtcacgc gtggcaaagt cctgccgggg tgcgggtcga tatcctgccg
     5761 gcggggccgg aaattctcga agccggagag attgtctggc ctaggactga tcgtcgatca
     5821 acccgcaaga gcaggcagtc attgaagact ttctgacgcg cctgtcgaac aaccagggcc
     5881 ctgaaaaccc tctagataag atggcgcgac aagggccgca cgtattccgc gacgatccgg
     5941 atgcgtggct tgcccgcggg gatgcgatgc tccgtggact ggcttccccg taggaagctc
     6001 aacttcagag cttccctact ccccccgaaa cgccttctcc agctcgccga tatccaactt
     6061 cttcatctcc atcatcgccg cgaacgcgcg cttggcgccc ggcgagttct catcgcgcag
     6121 catctcctcc atgccgttcg gcgtcacctg ccaggagagg ccgtacttgt ccttgagcca
     6181 gccgcactgc tgggcccggg ggtcgccgcc ggcgccgagc ttttcccagt agtagtcgac
     6241 ttcgtcctgg gtgtcgcagt tgatgatgat cgagacggcc tcgttgaact tgaaggcggg
     6301 gccgccgttc agggccatga agctctggcc gtccaggtcg aagttggcca ccatgaccga
     6361 gccggggggc ctcccgtgca cttcctggcc gacttcgctg tagcggaaga cgtcggtgat
     6421 gcgggaattc ctgaagatgg atgtgtagaa cttcgcggcc tcctcggcct ggtgatcgaa
     6481 ccagaggcac gtggtgaatc gcttgaaggt tgacatgggg tcctcgtgtt cgttgaggct
     6541 gtgcatgagc gaccgtgaac gagcggccgt cggcgcccgg cgcccgcttc cggggcgggc
     6601 agccgaaccc gtctgatggt ataacagtag tacgatgagc gatgacgcgc cggccgttcc
     6661 ccgcctggac ggccacgaca aggtgaccgg ccgggcccag tacgtggatg acatccacat
     6721 gcccgggatg ctgcacgggg ccaccatccg ctccacgatt gcccggggaa agatcaccgg
     6781 catcaccttc gacccggcgc tcgactggtc gggcttcacg atcgtgacgg cggccgacat
     6841 ccccggtaag aacgaagtcc tcctcctcac gaccgaccag cccttcctgg ccgacggtca
     6901 cgtgaggcac cacgccgagc ccatcctcct cctggcgcac gaagatcggg agatgctgcg
     6961 gcgggcggcc agggccatca cgatttccta cgccgaagag ccggcgtgcc tcgatgtgcg
     7021 ccaggcgctg cgccccgagt atctccagca tggcagggac aacacgtttc attcgatcga
     7081 catcgtcaag ggggatgtcg acaccgggtt cgaatccgcc gacctggtcc tcgacgaaac
     7141 gtacgaaacc ggcgcgcagg agcaggccta catcgagccg caggggatga tcgcgcggcc
     7201 cgcgccggaa ggaagcggca tcgagatctt cggctcgctg cagtgcccct actacgtggt
     7261 gaaggcagtc acgcacctgc tcggggttcc ggccgaggaa ctgcgcgtgg tgcagacgac
     7321 gacgggcggc gggttcggcg gcaaggaaga gtatccctcc atcattgccg ggcacgccgc
     7381 gctgctggcc ttgaagagcg ggcggccggt caagctggtg tacgaccgct acgaagacat
     7441 cctggccacc accaagcggc acccctcgat cacgcgcgtg aaggccggcg ccacgcgcga
     7501 cggcaggctc accgcgctgg aaatcgacct ggtgctcgac ggcggcgcct acgtgacgct
     7561 cacgcccgtg gtgctgtcac gggcgtcgct gcacgcgtcg gggccgtacg aggtgccgaa
     7621 catcacgatc cgcgcgcggg cccggctcac caacacgcct ccgcacggtg ccttccgcgg
     7681 attcggtgcg ccgcagtcga tctttgccgt cgagcggcac atcgaccggc tggcccggag
     7741 cctcgggatc gcgccgctcg aattccgtcg tcgcaattac ctgaaactgg agagcgtgat
     7801 ggccaccggc caggtggtgc gcgacgacat cgacatgcac gcgctggcca atgaagtgta
     7861 cgagcggctt ggcggcgccg cgcgcgaggc ggaactcgcg aaggccaatg cgcaatccac
     7921 ccacacgcgg tcggcgctgg gtttcgcagc cttctaccat ggcgccggct ttaccgggtc
     7981 gggcgaggag cggctggcaa gccaggtggc ggcgcgcctg gatgtggacg gccgcgtgga
     8041 aatcctctcc tccaatacgg agatcggcca gggaaccgtc accatcttca gcatcattgc
     8101 cgcgaaggcg ctcggcctcg aaccctccga gatccgggtg gcgccgcccg acacgtcgcg
     8161 cgtgcccgat tccgggccca ccgtggccag ccgcacggcg atggtggtgg gatggctggt
     8221 gcagcaggcg gtcctcgatc tcacggtgaa actggcggag aagtgcagcg actcgttgct
     8281 cgtcgtcgcg cccgaatcgc tccggggtga tgccttccgc cgggcggccg cgcaggccgt
     8341 ggagtccggt tgccgcatcg tcggaaggag caggtacgag aagccgtcct tcgtgacgtg
     8401 ggacgaagtc aactatcgcg gcgacgccta cgccacttat gcgtgggccg tgtacggctg
     8461 cgaggtcgag gtcgatacgc gcacgttcga ggtgagcgtg aagcgattcg tggccaacca
     8521 ggacatcggc ggcgtgctcc acaagacgct ggccgacggg cagatccagg ggggcgtggt
     8581 acaggggctc ggctgggcgc tctgcgagca ggtggtgtac gacaagggag ccgtgcgcaa
     8641 cgccgcgctc tcgacctacg tgatccccac gttcgtggat gtgccgccga tcgaggtcct
     8701 cttcgccacc acgccgtacg aattcggacc gatgggcgcc aaggggatcg gcgagctgcc
     8761 gatg
//
LOCUS       DASVOD010000312        18200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_804026, whole genome shotgun sequence.
ACCESSION   DASVOD010000312 DASVOD010000000
VERSION     DASVOD010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 18200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18200
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804026"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF720_06965"
     CDS             complement(<1..416)
                     /locus_tag="VF720_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879133.1"
                     /translation="MRIDERVPTSGGRMVRCVFSTMVLTVLAGPALAAVPAPYSLPWQ
                     LRPAGAGNVLRLDSVWSTYEGATRENGSTFVSLLLASGKISSSTALFGRFGIVQNDPP
                     VGESAAVPTNLVLGVSHAPKVSTPFRLACYLMVALPT"
     gene            607..1056
                     /locus_tag="VF720_06970"
     CDS             607..1056
                     /locus_tag="VF720_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX7879134.1"
                     /translation="MKILVGVDESEHGQQAIDWVRRMTLPKETEVVVVSVARTPMVLS
                     TEAYAPGLAYDDEALKGEFEFRTRIANEAATSLGKSGLTATTRVVTGDARLEIIDLAH
                     KEKCDLVVVGSHGRTGLAKLLLGSTASHVVTHAPCSVLVVKERKTPG"
     gene            complement(1084..2364)
                     /locus_tag="VF720_06975"
     CDS             complement(1084..2364)
                     /locus_tag="VF720_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012498859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7879135.1"
                     /translation="MGVRRVSAAEAVSTVRSGERVFIHGVAATPGVLVQALADRAAEL
                     RNVEIVHLHTEGEAPYAAPGLSASFRVNALFIGPNLRTAVAEGRADYTPVFLSDVPAL
                     FRGGALPIDVALVQVSPPDRHGFCSLGVSVDVALAAVQTARHVVAQVNPRMPRTHGDG
                     LIHVDAFHALVEVDNPLPERPPAVAGERELSIARHVAALIPNGATLQMGIGAIPDAVL
                     GALSGHRKLGVHTEMFSDGLIDLVERGVVTGEMKRVAPGRIVAGFVMGTRRLYDFVDD
                     NPLIAMHDIAWVNDTAVIRRNPAACAVNSAIEVDLTGQVCADSIGERMFSGVGGQMDF
                     IRGSALSEGGRPIIALPATTSSGESRIVSILKPGAGVVTTRAHVHFVVTEHGVADLHG
                     RTLRERARALIAIAAPEHREALAAAARRRLGASL"
     gene            2413..3126
                     /locus_tag="VF720_06980"
     CDS             2413..3126
                     /locus_tag="VF720_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7879136.1"
                     /translation="MSPKARPDRCLLCEARSKNAMCQLPPDAFQRFFAGSRVESLPAG
                     TVLFRQGDEAADIFIVRTGQVKVLFQHADGMEQLLRVAGPGDMLGVARRIGDGMAVTA
                     VAKKGVSICRTRVDQLERTLRADADIALAWCHLLADDAERTREALFLHGPQPAATRLA
                     RLLLQTWQRNHQRDTTESRAVHLTQGEIAAALSVVEETITRLLATLEEKGVVAPGRGR
                     IDVLDPDRLAALAGHDPPS"
     gene            3123..5072
                     /locus_tag="VF720_06985"
     CDS             3123..5072
                     /locus_tag="VF720_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005371118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7879137.1"
                     /translation="MTLRGTDERIAALAVAAIAFHLALRWGAPAFAPAGGLPEFTPLA
                     GIPTRDLPLLVALVIGGGWLVFGLVLGLVRGRFSSDLLAGLSIVTSVLLGEYLAGVVV
                     VLMLAGGQALETYAARRASSVLAALAKRMPSTAHRLETNGDRQADVPGEAERLVDIPL
                     DQVAIGDRLTVYPHETCPVDGTVVSGHGSMDESYLTGEPYRLSKAPGTRVLSGAINGE
                     TALVIRADRLAVDSRYASIMAVMRESEQRRPHIRRLADRLGTVYTPLAVGLALIAWWW
                     SGEATRFLAVLVVATPCPLIIAIPVAIIASISLAARRGIIIRDPSVLETIDSCRTALF
                     DKTGTLTYGQPELVAIETLPGYAEPEVLALAAALERYSRHPLARAILDRAGRSDVPVL
                     EAVAVSEPPGQGLTGRVGDRDVRLTGRSAFTRESPDLAQLLPPHTGGMECVVIVGSRP
                     AALFRFRDEPRGDSSRFIRHLSARHGIRRLILISGDRESEVQVLAGRVGITEVHAGQS
                     PEQKVTRVRTETALAPTLYLGDGINDAPALTAATVGIAFGQASDVTSEAAGAVVLDSS
                     LQRVDELLHIGRRFKRIALQSAVGGMALSIGGMILAAVGLLPPVGGAVFQEVIDVAAV
                     LNALRAAAPPASLTDYGPGTAADGK"
     gene            complement(5039..5794)
                     /locus_tag="VF720_06990"
     CDS             complement(5039..5794)
                     /locus_tag="VF720_06990"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015277.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEX7879138.1"
                     /translation="MLSVIGCTGGGKSSTASRPAARATPAPWNGQVNVAGRLRAMFHE
                     GMTGTTVALDSLLPDTTLYAVGTLSDLAGEVTIARGVAYLSRPQSADSATTEVATASS
                     AGATLLVWSRVPAWIPVPVDSMIPFEEIDARIATMAASTGLNVTGRFPFLVTGPLESI
                     DWHVVDGRLLEGGGESHADHLAASTRLHRDHVSGTIIGFYSTHDEGVFTHMGSATHLH
                     VALDGPAASGHVDHVVIPAGAVIHFPSAAVPGP"
     gene            complement(5962..6348)
                     /locus_tag="VF720_06995"
     CDS             complement(5962..6348)
                     /locus_tag="VF720_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879139.1"
                     /translation="MDKLTYTIDIPARIVRLDYQASPDWPEMQEVLERLLADPSHRPG
                     MGILADRSHVDTPQTVGYIRQIMAYVRKHQSQFGDARWGLVVRGAGPEGMGKLAQVLG
                     DGIPTMELELFDNTADAEAWLRSKPA"
     gene            complement(6341..7486)
                     /locus_tag="VF720_07000"
     CDS             complement(6341..7486)
                     /locus_tag="VF720_07000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7879140.1"
                     /translation="MSAFLRRLVHELRTPLASMATVVDLLDRRDRPTVDLEETDRQAR
                     DILRRQVRLMLRRLDELSDFTRIVDGRLRLEPAAVDAAELVVEEVEAHRSRAIEDGRT
                     LVIDVAAPPPSTAADGKRLRQIVRHLVDNALRHTQRGGRVEVSIDHDERHARIRVSDD
                     GDGIEPTRLPSVFEDWRRVDSNIGGHGRLGLGLAMARHLAELHGGSLEVESAGAGQGS
                     AFVLRLPIIDPADIPPDGLTASMGSPARPASTRPLRILVVEDHRFFGEAFAQALEQMD
                     HAVSVVPAAEEALALLATLRPDIIFSDISMPGMHGHDLARALRETDGGADLVLVALTG
                     SGDPEDRARSLAAGFDLHLVKPPDFEVLRQLFEDVTRRRYGGDQEHG"
     gene            complement(7609..7818)
                     /locus_tag="VF720_07005"
     CDS             complement(7609..7818)
                     /locus_tag="VF720_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012308354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEX7879141.1"
                     /translation="MTTEPVIELQDWLKWQQWVSSGGEAKVVIQGGQVRLNGEVETRR
                     RKKLHAGDLIEFEGRSGRVETPQAG"
     gene            8070..9098
                     /locus_tag="VF720_07010"
     CDS             8070..9098
                     /locus_tag="VF720_07010"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7879142.1"
                     /translation="MRADGDATHRRRFLARVDSLRQAAHIPGLAVAVVNDTTILFTRE
                     FGFADIDRELPVTLNTPFNIASVTKPLSAVVALKLVELGKLDLDRPMHSYAGFNEFCA
                     DTRGAESIFFRDFHCGDSTMSLRHVMSMEVNGAPGTRFFYNPVVYSWASRPMMEATGE
                     TFSDLMAQYVFRPAGMTQSARQNRKLPLREDLAAGLARPYRWDDSLGVIVPSELPGPQ
                     GDGAAGGVIATAHDLARFDIALTQGKLIGSASRNEMWTAGHSPDGATLPYGIGWFVTE
                     IDGEPVCWHTGLWEGAYSALYARFPGRKLSVIFLANSDGLRWDNALDEAALERSPFMK
                     LFVEEMLR"
     gene            complement(9101..9970)
                     /locus_tag="VF720_07015"
     CDS             complement(9101..9970)
                     /locus_tag="VF720_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879143.1"
                     /translation="MSASTSLGATALFLSIVMAAADPAAAVNLVPNQSFEGYTPCPSG
                     LSEIANAAPWDTPNQASPDLYNVCATAGSGVSVPSNTFGSQAALTGVGYAGIIVRPIN
                     DYREYLEVPLATALTASVSYTVSFNLSLCDLSNITCDRIGAYLSVGSVGPVPGVGTLP
                     FTPQIESAPGNFLTDKTNWITVSGTYMAAGGENSIVIGNFHDNATTNLTSLSGSYNGV
                     YFYVDDVLVEANTVTDEACCLPDGSCGLMLPGECQSLGGSPQGPGSTCGPDACHPVAV
                     APSTWSGIKARTE"
     gene            complement(9973..10896)
                     /locus_tag="VF720_07020"
     CDS             complement(9973..10896)
                     /locus_tag="VF720_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879144.1"
                     /translation="MLRRYRAILLPLALILLAVPTARPARAAGGVGDAFVTSDASDVV
                     RAYTGGTGNYIGIHSTGVLGDGQLAIHFGLNNGRVLVGSFGGGVDEYVAATGAYIKTY
                     APGGGWQWAGLYGPNGNVYIGSFATGDVREYDVNTGALVGILYLIPDPSDMNIGPNGN
                     LYICQYQAGAVTEIDFAGNFVSQWFQPPNSRSQDIEFLPGGGFVVPVMGNNVAYRYNA
                     AHVINGVYSGTGWQRPHGVDLNPNDGLLYIVDGVTMQVHVFNPLTMVELNANWLSPGP
                     GDKIVDIEFRQDQQIVPVLPSTWSKVKQLGQ"
     gene            complement(11003..11653)
                     /locus_tag="VF720_07025"
     CDS             complement(11003..11653)
                     /locus_tag="VF720_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879145.1"
                     /translation="MYVDTNHAARHDADAASPRARPRIVVIDPNADAWSGTDLVAARY
                     DMTLALDPRRGVEMARRRGADCVVAEVSPHGKPRYDLVGELRRDARTRAVSVILIAGR
                     EGASTALEAVRSGADDYLVRPFLPAELVTRIEMQLRWRRHQAAETAERRRLADLLHSD
                     LQQLLVAAEMRLGMAANRRAHEEMVREVAQAREILARARRSAQELNRQLRPLEPND"
     gene            11936..12127
                     /locus_tag="VF720_07030"
     CDS             11936..12127
                     /locus_tag="VF720_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879146.1"
                     /translation="MYRKTLPSAPPDYLSRHERTQLELLEIKKQTDLAVDGTLALLAV
                     GVLMFLVLVGLITLFRAVG"
     gene            complement(12164..12850)
                     /locus_tag="VF720_07035"
     CDS             complement(12164..12850)
                     /locus_tag="VF720_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7879147.1"
                     /translation="MMMTKTETSTARRPIKVVLIDDHPIVRQGLRDLIANETDIEVCG
                     EAGSISTALEVIQECAPNVAVVDLTLGTESGLDLVKDITGRFPEVKVLVLSMHDEAFY
                     AERILRAGALGYVNKSEPGEKLIRAIRRASGGQAVLSSATTVHFLRRGAGGSAPAKSG
                     VANLSDRELQVFELVGAGLSSRDIATQLCVSVKTVESHLEHIKVKLDVSSGRELVRRA
                     TMWVEGNHKP"
     gene            complement(12886..14172)
                     /locus_tag="VF720_07040"
     CDS             complement(12886..14172)
                     /locus_tag="VF720_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7879148.1"
                     /translation="MTDSSSARMQLEIDRLTGELDRTRRRLLRCRVDLAAVRGASGNG
                     SGTVPSDLSFLRDIAVAGNSRSASVEEMLAYTLRRTCEHYGWEFGVAFRSTWGLPSAL
                     GLTPPIVHATDPVRFMSLERLVRAEAAPLSDRLPVRAFSESRPLWSDDLSANLPAPMA
                     AAAVQAGLRMIMVFPVTAGSEPVAVLKFYLSNVIEPDDDMLDTMSGVCTQLAHVIQRD
                     DLARQLLELTVYEQDRVTRDLHEAVGQELAGLALTVESMSRRESGNGGQRQTLERVAG
                     GLRQVLVDIQSVARGLVRVPDRANALGEMLVELGWLAERNSSTQMTCVVEVDPVIDMK
                     DPDVTNHLYRIAQEALQNAISHSRCRRVRIRLWQDEQRISLDVDDDGLGIAEHAIRGQ
                     GMRSMYQRARLIGGRLEIQGLDGTHVHLSVPRIGVG"
     gene            14725..16596
                     /locus_tag="VF720_07045"
     CDS             14725..16596
                     /locus_tag="VF720_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7879149.1"
                     /translation="MKVSHRGILGLAAAIALGLLAGSTIPAVMAVAGNDKNRHDDEKK
                     NDNRKDKDKDDDDDDDDDRNGPGSLRDVVLPGGYKIREFAGGLNFPTGITWDSKGNVF
                     VSEAGIADTPARIVRIDEDADVVPVANGFTAPVTDVTWHDGWLYVSHLGTISRIRPDG
                     SGREDVVTGLPAFGDHSNADIIFGPDGRMYFSIGTATNSGVVGPDNSWVMDHPEVHDI
                     PAETITVRAENYTSPNIMTSTPNDVTLTGPFQPFGEAAALGEVVPGEVKSNGSILSAN
                     PDGSDLQVYAWGFRNAFGLGFDESGQLWATNNGMDERGSRPIEGDLDAFFQVEQGDWY
                     GWPDFSLGRPVNQELFEVDGVIPELLLLSHPPLSPLADAFAVFEDHVSADKFDFSSSS
                     RFRYEGDAFVAETGSLPTLTGAADYYGYRVTRVDHRTGDVSVFLANKSGKPAFVTGED
                     GINKPIDVKFAPDNADVMFVVDFGAFLPTPATEEEFPSVIEPGSGTIWVVGRASTLNE
                     FARNQGDDDEDDDGDDGEEEIDGAPGTGLLLNTSVTAGPTINYRLESPQIVSLRVFDV
                     TGRVVRTLVEEPQVAGDHVVAWDGQDDQFAAVPSGVYFYRIEAGADRQSGKVLITAP"
     gene            complement(16600..>18200)
                     /locus_tag="VF720_07050"
     CDS             complement(16600..>18200)
                     /locus_tag="VF720_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7879150.1"
                     /translation="DPDELHDALLVSGTLTDEEMARWPEWRDRLEAAGRTARQGDLWL
                     AAERRELSDVERVRGRLEIAGPVTAEQLAAMIRLPRAAVDAALLAIEADGVVLRGHFT
                     PGVEDLEWCERRLLARIHRYTISRLRSEIEPVTPAQFSSFLFEWQHVTPDHRTAGLEG
                     LAAVLGQLEGFEVPAGAWETDVLSARSVDYDPQLLDSLSLMGRVMWARVSPPSPSLRG
                     AISGPLRSTPIALLRREHLGAWHALAGDVERTPRGANAALVLSVLDAGGAMFFHDLVT
                     RTGLLGTQVEGALAELAALGLVTADGFTGLRALITPESRRPPIAGASRRRHRSIAFGV
                     ESAGRWARLAGSSGGPDSADPGHGATNTGREAAVEHLARTLLNRYGVMFRKLLAREAF
                     SVPWRELLRTYWRLEARGEVRGGRFVSGIAGEQFALPDAVTRLRAVRRAGPQGQLTAL
                     SAADPLNLTGILSPEDRVAALAGNRIVYEDGIPVVVKEGGETRWLAALPPERERLAEE
                     ALIRKRVSASLRARLGIPRAISSR"
BASE COUNT         2986 a         6156 c         6181 g         2877 t
ORIGIN      
        1 gtcggcagcg ccaccatcag atagcacgcc agccggaacg gcgtcgagac cttgggcgcg
       61 tgggaaacgc ccagcaccag gttggtcggc acggcagcgg attcgccgac cggcggatcg
      121 ttctgaacga tgccgaaccg cccgaagagc gcggtggacg agctgatttt cccgctggcc
      181 agcaagagcg acacgaaggt cgatccgttc tctcgggtag ccccctcgta ggtggaccag
      241 accgaatcga ggcgcagcac gttccccgcg cctgccgggc ggagctgcca cggaagggaa
      301 tagggcgccg gtacggccgc caaagcaggc cccgcgagca ccgtcaggac catcgtgctg
      361 aaaacgcacc ggaccatccg gccacccgac gtcggaaccc gttcatcgat tctcatccac
      421 acctcccggt cattgtgacc acacgaggat gatggcgcgc ggactcgaca cgcttcccga
      481 caattgtcag gtttcggacc gtatcaacgc accccagggc gatttgagtc gtcattatct
      541 tggaagagag ttgaccggag ttcaggggac cccacgagga gtggctcccc ggggaaggag
      601 actcaagtga agatcctggt cggagtcgac gagtccgaac acggacaaca ggcgatcgat
      661 tgggtgagac ggatgacgct gccgaaggag acggaggtcg tcgtcgtgtc ggtggccagg
      721 acgccaatgg tcctgagcac cgaagcctac gcgccgggcc tggcctacga cgacgaggcc
      781 ttgaagggtg agttcgaatt ccggacgcgc atcgccaacg aggcggcaac cagcctgggt
      841 aaatccgggc tgaccgcaac gacccgcgtg gtcaccggcg atgcgcgcct ggagatcatc
      901 gacctggcgc acaaggaaaa atgcgacctg gtggtggtcg gatcccacgg acgcaccgga
      961 ctggccaagc tcctcctcgg gagcacggcg tcgcacgtcg tcacccacgc cccgtgcagc
     1021 gtgctggtgg tcaaggagcg aaagacaccg ggctgagagg tgtaggggcc cggaggtgcc
     1081 gggtcaaagg gaggccccca gccgccgccg ggcggcggcg gcgagagcct cccgatgctc
     1141 gggcgcggca atcgcaatca gcgcccgggc ccgctcccga agagtgcggc catggaggtc
     1201 cgccacacca tgctccgtga cgacaaagtg aacatgggcc cgcgtcgtga cgactcccgc
     1261 gccgggcttg agaatggaaa cgatgcgcga ctctccgctg gaagtggtgg ccggaagcgc
     1321 aatgatcggc cggcctccct cggaaagcgc cgaaccgcgg atgaagtcca tctgtccgcc
     1381 cacgccggaa aacatccgct caccgatgga gtcggcgcac acctggcccg taaggtcgac
     1441 ctcgatggcg ctgttcacgg cacaggccgc cgggttgcgt cggatcaccg ccgtatcgtt
     1501 cacccaggcg atgtcgtgca tggcgatgag gggattgtcg tccacgaagt cgtaaagccg
     1561 gcgcgtgccc atgacgaatc cggccacgat gcggccgggg gccacccgct tcatctcgcc
     1621 ggtcacgaca ccgcgctcga ccaggtcgat gagcccgtcg gaaaacattt ccgtgtggac
     1681 gccaagcttc cggtggccgg agagggcgcc gagcaccgcg tccgggatgg cgccgatccc
     1741 catctgcagg gtggccccgt tcgggatgag cgccgcgaca tgccgggcaa tcgaaagctc
     1801 ccgctcgccg gccaccgcgg gggggcgctc gggaagcgga ttatccacct ccacgagcgc
     1861 atggaaggcg tccacgtgga tcagcccgtc tccgtgcgtg cgcggcatcc tcggattgac
     1921 ctgggccacg acgtgccggg cggtctggac cgcggccagg gccacatcga ccgacacgcc
     1981 gagcgagcag aacccatgac ggtccggcgg cgacacctgg accagcgcca cgtcgatcgg
     2041 cagcgccccg ccgcgaaaga gggccggcac gtcgctgagg aagaccggcg tgtagtccgc
     2101 ccgcccctcg gccacggcgg tgcgcaggtt cggtccgatg aacagcgcat tgacccggaa
     2161 gctcgccgag aggcccggcg ccgcataggg cgcctccccc tcggtgtgca gatgaacgat
     2221 ttcgacgttc cgaagctccg cggcccggtc ggccagggcc tgtaccagga cacccggggt
     2281 ggccgcaacc ccgtgaatga aaacccgttc acccgaccgg acagtggaaa cggcctccgc
     2341 ggccgagacg cggcgaactc ccacggttat cgcactcctt tcccatccgc agttcggata
     2401 gaatcgagga tcatgagccc gaaagcgcgt ccggatcgct gcctcctgtg cgaggcgcgc
     2461 tcgaagaacg ccatgtgcca gctgccgccc gatgcgttcc agcggttctt tgccggctcc
     2521 cgagtggaat ctcttcctgc gggtaccgtg ctcttccgcc agggcgacga agcagccgac
     2581 attttcatcg tccgcaccgg ccaggtaaag gtcctcttcc agcacgccga cggaatggag
     2641 cagcttctgc gcgtggcggg gccgggcgac atgctcggcg tggcgcgccg catcggcgac
     2701 ggcatggccg tgacggccgt cgccaagaag ggcgtcagca tctgccgcac ccgcgtggac
     2761 cagctggagc ggaccctccg cgcggacgcg gacatcgccc tcgcctggtg ccacctcctg
     2821 gccgacgacg ccgaacgaac gcgcgaggcg ctcttcctcc atggcccgca accggcggcc
     2881 acgcggctgg cgcggctcct gctgcagacc tggcagcgga accatcagcg ggatacgacg
     2941 gagtcgcgcg ccgttcacct gacgcagggg gagatcgccg cggcgctgtc ggtggtggag
     3001 gaaaccatca cgcgcctgct cgccacactc gaggaaaagg gcgtggtcgc gcccggacgt
     3061 ggacgcatcg acgtcctcga tccggaccgg ctggcggcgc tggcgggcca cgacccgcca
     3121 tcgtgacgct ccgggggacg gacgaacgga tcgccgccct ggccgtggcg gcgattgcgt
     3181 tccacctcgc gcttcgatgg ggtgctcccg ccttcgctcc agcgggagga ttgcccgagt
     3241 tcactccgct ggccggcatt cccacccgcg atctcccgct tctcgtggcg ctggtgattg
     3301 gtggcggatg gctggtgttc ggcctcgtcc tcgggctcgt gcgcggccgg ttcagctcgg
     3361 acctcctggc cgggctctcg atcgtcacgt ccgtgctgct gggcgaatac ctggccggcg
     3421 tggtcgtcgt cctgatgctg gccggtgggc aggcgctcga gacctacgcg gcgcgccggg
     3481 cttcatcggt gctggccgcg ctggcgaagc gcatgccgtc gaccgcccat cggctggaaa
     3541 caaacgggga ccgccaggcg gacgtacccg gcgaggcgga gcgcctcgtc gacatccctc
     3601 tcgaccaggt ggccatcggc gaccgcctca cggtgtatcc ccacgaaacg tgcccggtgg
     3661 acggcacggt ggtgagcggc catggcagca tggacgagtc gtacctgacc ggcgagccgt
     3721 atcgactctc caaggcgccg gggacgcgcg tcctctcggg cgccatcaat ggcgagacgg
     3781 cactcgtcat ccgggccgat cgcctggccg tcgactcacg ctacgccagc atcatggcgg
     3841 tgatgcgcga gtccgagcag cgccgcccgc acatccggcg gctggcggac cgactcggta
     3901 ccgtctacac accgctggcg gttggcctgg cactcatcgc ctggtggtgg agcggcgagg
     3961 ccactcgttt cctggcggtc ctggtggtgg ccacgccgtg tccgctcatc atcgccatcc
     4021 cggtggcgat catcgcgtcg atctcgctgg ccgcgcgacg gggcatcatc atccgggatc
     4081 cgtcggtgct ggagacgatc gacagctgcc gcacggcgct gttcgacaag acgggcaccc
     4141 tcacttacgg ccagccggaa ctggtggcaa tcgagacgct tccggggtac gccgagccgg
     4201 aggtgctcgc cctcgccgcg gcgctggagc gctattcccg tcacccgctg gccagggcca
     4261 ttctcgaccg ggccgggcgg tccgacgttc cggtcctgga ggcggtggcg gtgagcgaac
     4321 cgccggggca gggattgacg ggccgggtcg gcgatcgcga cgtgcggctc accggccggt
     4381 cggctttcac gcgggagagt cccgaccttg cccaactcct gccgccccac acgggcggga
     4441 tggaatgcgt ggtgatcgtg ggttcacgtc cggccgcgct gttccgattt cgtgacgagc
     4501 cccgcggcga cagctcccgc ttcatccgtc acctgtcggc ccggcacggc atccggcgcc
     4561 tcatcctgat ttccggtgat cgcgagtccg aggtacaggt tctcgccggc cgggtgggta
     4621 tcacggaagt gcacgccggc cagagtccgg agcagaaggt aacccgtgtg cggacggaga
     4681 ccgccctcgc ccccaccctg tacctcggcg acggcatcaa cgacgcgccc gcactgacgg
     4741 ccgccacggt cggcatcgcc ttcggccagg ccagcgatgt cacgagcgag gcggcgggcg
     4801 ccgtcgtact cgacagctcg ctccagcgcg tcgacgaatt gctccacatc ggcaggcgct
     4861 tcaagcggat cgccctgcag agtgccgtcg gagggatggc cctgagcatc ggcggcatga
     4921 tcctggccgc cgtcggcctc ctgccgccgg tggggggcgc ggtgttccag gaagtcatcg
     4981 acgtcgcggc cgtgctgaac gcgcttcggg ccgcggcccc gcccgcgtcg ttgacggact
     5041 acggcccggg aacggccgcg gacggaaagt gaatgaccgc cccggccggg atgaccacgt
     5101 gatccacgtg gcccgaagcg gcgggaccat cgagcgccac gtgcagatgg gtggccgatc
     5161 ccatgtgcgt gaacacccct tcgtcgtgcg tcgagtagaa gccgatgatc gttccggaaa
     5221 cgtgatcgcg atgcagccgc gtggaggccg ccaggtgatc ggcgtgtgac tcgccgccgc
     5281 cctcgagaag gcgcccgtcc acgacatgcc agtcgatgga ttcgagcggg ccggtgacga
     5341 gaaacgggaa acgcccggtg acgttcaggc cggtggaggc ggccatcgtc gcgatacggg
     5401 cgtcgatctc ctcgaagggg atcatggaat cgaccggcac cgggatccac gcgggaactc
     5461 gcgaccagac cagcagggtg gcgccggcgc tcgatgccgt ggcgacctcg gtggtcgccg
     5521 aatcggccga ttgcggccgt gacaggtacg ccacgccgcg cgcgatcgtc acctcgccgg
     5581 ccaggtcgga cagggtgccg acggcgtaca gcgtggtgtc ggggagcagc gagtcgagcg
     5641 cgacggtcgt gccggtcatc ccttcgtgga acatggcacg gagccggccg gctacgttga
     5701 cctggccgtt ccagggagcc ggggtggcgc gagcggccgg tcgactggcg gtgctcgatt
     5761 tcccaccgcc ggtgcatccg atgacggaga gcattacgcc cccccaggca atggcgacga
     5821 aaatcaacga agcgggtcgt ccggcgggcc cggttggttg tgaactcatc cgcatgtctt
     5881 gcattgtaag acacgcgcgc gaatcaataa cggcgcgcgc caacataagt gtcagacagt
     5941 tcgacggcgc cccacccgcg gtcacgccgg cttcgatcga agccacgcct ccgcatccgc
     6001 cgtattgtcg aagagttcga gttccatggt gggaatgcca tcgcccaaca cctgggcgag
     6061 ctttcccatt ccttcggggc cggcgccgcg aacgaccagg ccccagcggg cgtctccgaa
     6121 ctgtgactgg tgcttgcgca cgtacgccat gatctggcgg atatagccga cggtctgcgg
     6181 cgtgtcgaca tgcgatcgat cggccaggat ccccatgccc ggacggtggg agggatcggc
     6241 cagcaggcgc tccagcactt cctgcatttc gggccagtcg ggggatgcct ggtagtcgag
     6301 tcgcacaatt cgagccggga tatcgatcgt gtaggtcagc ttatccatgc tcctggtcac
     6361 ctccgtaccg gcgacgcgtc acgtcctcga acaactggcg aagcacctcg aaatcgggcg
     6421 gtttgacgag atggagatcg aagccggctg ccagcgagcg ggcgcgatct tccgggtcac
     6481 cgcttcccgt gagggccacg agcacgagat cggcaccacc gtccgtttcg cgcagggccc
     6541 tggccaggtc gtgcccgtgc atgccgggca tcgagatgtc ggagaagatg atgtcggggc
     6601 gcagcgtcgc cagcagggcc agggcctcct cagcggctgg aacgaccgac accgcatggt
     6661 ccatctgctc gagcgcctgg gcgaatgcct ccccgaagaa gcggtgatcc tccacgacca
     6721 gaatgcggag cggccgggtt gaggccggcc tcgcggggct tcccatcgag gcggtgagtc
     6781 cgtcgggcgg gatgtcggcg ggatcgatga tgggaagccg cagcacgaag gcgctcccct
     6841 gccccgcgcc cgcgctctcg acctcgagcg acccgccgtg caactccgcc aggtgacggg
     6901 ccatcgccag gcccaggccg agtcgcccgt gccctccgat gttcgagtcc acacgacgcc
     6961 aatcctcgaa gaccgatggg agccgggtgg gctcgatgcc gtctccgtcg tccgaaaccc
     7021 ggattcgcgc gtggcgctcg tcgtggtcga tcgatacctc gacccggccg cctcgttgtg
     7081 tgtgacgaag ggcgttgtcg accaggtggc ggacgatctg gcggagtcgc tttccgtccg
     7141 cggcggtgct cggcgggggc gccgccacgt cgatgacgag cgtgcgccca tcctcgatgg
     7201 cccgggagcg gtgtgcctcc acctcctcga cgaccagttc cgcggcatcc accgccgccg
     7261 gctcaaggcg cagccgaccg tccacgatgc gggtgaagtc cgacagctcg tcgagccggc
     7321 gcagcatcag ccgaacctgg cggcgcagga tgtcccgcgc ctggcgatcc gtctcttcca
     7381 gatcgacggt gggccggtca cgccgatcca gcaggtcgac gaccgtggcc atcgacgcca
     7441 atggcgtgcg gagttcgtgc acgagccggc gtagaaacgc ggacatggcc ggggcgccag
     7501 ccccggcagg tgacgatgcg gctgtcgagt cggtcaatcc atcccccttg gtacccggag
     7561 ggacgctatc agatgaggaa gccgaccggg atgggaatca gggtcgattc agcccgcctg
     7621 cggagtttcg acccgacccg accgtccttc gaactcgatc aggtcgccgg cgtgtagttt
     7681 tttcctccgt cgggtctcga cttcgccgtt gagccgcacc tggcccccct ggatgacgac
     7741 cttggcctcc ccaccgctgg acacccactg ttgccacttc agccaatcct gtaattcgat
     7801 gaccggttcc gttgtcatgg gggaagtcta cggcctggcc aaccaggttc aaaccgggtg
     7861 gcgcatgcac cggtgcatgt tgttatgtaa acctgggtgg gacggtgtcc gcaacctgcc
     7921 gcgcccgaac gagtaccatg ccccccgtga cgaaccccct cccgaagcgc ccgcgatccc
     7981 gcatcccgcg acacctgttc gcattcctcg ctccggtcgc tttactcctg ctgatcaacc
     8041 tcgccggctg tgagcgacag aggccgccga tgagggccga cggggatgcc acgcatcgcc
     8101 gccgcttcct cgcgcgcgtg gattcgctgc gacaggccgc ccacatcccc ggcctggccg
     8161 tggcggtcgt gaacgacacc acgatcctgt ttacccggga gttcggcttc gccgacatcg
     8221 accgggaact cccggtaacc ctgaacaccc ccttcaacat cgcctccgtc acgaagcccc
     8281 tttcggcggt cgtggcgctc aagctggtgg aactcggcaa gctggatctc gatcggccca
     8341 tgcacagcta cgccgggttc aacgaattct gcgccgacac gcgcggtgcg gagtcgatct
     8401 tcttccgcga tttccattgc ggcgactcga cgatgtcgct ccgccacgtg atgtcgatgg
     8461 aagtgaacgg cgcgccgggc acccggttct tctacaaccc ggtcgtctac tcgtgggcct
     8521 cacgccccat gatggaagcg accggtgaga ccttttccga cctgatggcg caatatgtct
     8581 ttcggccggc cggcatgacc cagtccgccc gccagaaccg gaaactcccc cttcgcgagg
     8641 acctggcggc cgggctcgcg cgcccctacc gctgggacga ttcgctcggg gtcatcgtcc
     8701 cctccgaact tccggggcca cagggggatg gcgcggcggg cggcgtcatc gccaccgctc
     8761 acgacctggc gcggttcgac atcgcactga cacagggcaa actgatcgga tccgcttcgc
     8821 ggaacgagat gtggacggcc gggcactccc ccgatggcgc cacgctcccc tacggaatcg
     8881 gctggtttgt cacggaaatc gacggcgagc cggtctgttg gcataccggc ctgtgggaag
     8941 gggcgtactc ggccctctac gcccgcttcc cggggcgaaa gttgagcgtc atcttcctgg
     9001 cgaactcgga cgggctccgc tgggacaacg cgctggatga ggcggcgctg gagagatcgc
     9061 cgttcatgaa gttgttcgtg gaggagatgc tgcggtagcc ctactccgtc cgcgccttga
     9121 tcccgctcca cgtgctcggg gccaccgcca ccggatgaca cgcatccggc ccgcatgtcg
     9181 agccggggcc ctgcggtgaa ccgccgagcg actggcattc accggggagc atgaggccgc
     9241 acgaaccgtc cgggaggcag caggcttcgt cggtgaccgt gttggcttcc accagcacgt
     9301 cgtccacgta gaaatagacg ccgttgtacg agccggacag gctggtgaga ttggtcgtgg
     9361 cgttgtcgtg gaaattgccg atcacgatgg agttctcgcc ccccgcggcc atgtaggtgc
     9421 ccgacacggt gatccagttg gtcttgtcgg tcaggaagtt tccgggcgcg ctctcgatct
     9481 ggggggtgaa cggcagcgtg ccgacgccgg ggacggggcc caccgaaccg accgagaggt
     9541 acgcgccgat gcggtcgcag gtgatgttcg agaggtcgca cagcgacagg ttgaaactga
     9601 ccgtgtacga cacgctggcc gtgagcgcgg tggccagcgg tacctcgagg tactcgcggt
     9661 aatcgttgat gggacggacg atgatccccg cgtaccccac gcccgtcagc gccgcctggc
     9721 tgccgaaggt gttggacggc acgctcacgc ccgagccggc cgtggcgcag acgttgtaca
     9781 ggtcggggga cgcctggttg ggcgtatccc agggcgcggc gttggcgatc tccgaaaggc
     9841 ccgagggaca cggcgtgtac ccctcgaagc tctggttcgg aacgagattg acggccgcgg
     9901 cgggatccgc cgcggccatc acgatcgaaa ggaatagcgc ggtagcgccg agcgatgtgg
     9961 aggcgctcat ggctactgac ccagctgctt gaccttgctc caggtgctcg gcaggaccgg
    10021 cacgatctgc tggtcctggc ggaactcgat gtcgacgatc ttgtctcccg ggcccggtga
    10081 gagccagttg gcgttgagct ccaccatggt caggggattg aagacgtgca cctgcatcgt
    10141 gacaccgtcg acgatgtaga ggaggccgtc attggggttc aggtccacac cgtgggggcg
    10201 ctgccagccg gttccggagt acacaccgtt gatgacgtgg gccgcgttgt agcggtaggc
    10261 cacgttgttc cccatgaccg gcacgacgaa tccgccgccc gggaggaact cgatgtcctg
    10321 ggagcggctg ttggggggct ggaaccactg gctcacgaag ttgccggcga agtcgatctc
    10381 ggtgacggcg cccgcctggt actggcagat gtagaggttg ccgttcggcc cgatgttcat
    10441 gtcggacggg tcgggaatga gatagaggat gccgaccagg gcgccggtgt tcacgtcgta
    10501 ctcgcgcaca tcacccgtgg cgaacgagcc gatgtacacg ttgccgttgg ggccgtacag
    10561 gccggcccac tgccagccgc cgcccggcgc ataggtcttg atgtaggcgc cggtggccgc
    10621 cacgtattcg tccacgccgc cgccgaagct gcccaccagg acgcgcccgt tgttcaggcc
    10681 gaaatggatc gccagctggc cgtccccgag gacgcccgtc gagtggatgc cgatgtagtt
    10741 gccggtgccc cccgtgtagg cgcggaccac gtccgaggca tcgctcgtga cgaaggcgtc
    10801 gccgacgcca ccggccgcgc gggccgggcg ggccgtcgga acggcgagga ggatcagggc
    10861 gaggggaagc aggatggcgc gatagcgacg gagcatgggg gtcctcctcg atgtggggca
    10921 gcacggacga ggtaatggcc cggatggcag gcgccacacg cggccccgga gaccgccaag
    10981 aatagcagaa aatagccgct tttcagtcgt tcggttcgag cgggcggagc tggcgattca
    11041 attcctgggc ggatcggcgg gcccgggcca ggatctcgcg ggcctgggcg acctcgcgaa
    11101 ccatctcctc gtgggcgcgg cgattggccg ccatccccag ccgcatttct gcggccacga
    11161 ggagctgctg caggtcgctg tggaggaggt cggcaagccg ccgccgctcg gccgtttccg
    11221 ccgcctggtg gcgccgccac cggagctgca tctcgatccg ggtgacgagt tcggccggaa
    11281 ggaaggggcg gaccaggtag tcgtcggcgc ctgaccggac ggcctcgagc gcggtcgagg
    11341 cgccttccct gccggcgatc aggatcaccg agacggcgcg cgtgcgggcg tcccgtcgca
    11401 gctctcctac caggtcgtat cgaggctttc cgtgcggtga cacctcggcc accacgcaat
    11461 cggcaccgcg acgccgcgcc atctcgacgc cacgccgggg atcgagtgcg agtgtcatgt
    11521 cgtagcgggc cgccacgaga tcggtgccgc tccacgcgtc cgcattcgga tcgatcacga
    11581 cgatgcgcgg ccgggcccgg ggagaagcag cgtcggcgtc gtgtcgagcg gcatggttgg
    11641 tatcgacgta catgctgaca tgcctcgagg gtgtcgattt cccgagcgcc gccccgccgg
    11701 aagaattgaa tgagaggaag accgtaagcc cgccgagccg cctcccgcaa ggaattttcg
    11761 acggcaaacg tgtgtcgcga cgcgaaccga tcggacgacc ggaacaaatt acccacttcc
    11821 gcaaatcgca caccagaatc aggaatgtcc cgatttttcg cgatccgggg agcgaccatg
    11881 ctgtcactcc tgtaaccgga tttcgatctc cacccacccc aatggagaga gcgccatgta
    11941 ccgcaagacc ctgccgagtg ccccacccga ctacctctca aggcatgagc gcacccagct
    12001 cgagctgctc gagatcaaga aacagaccga cctggcggtc gatggcacgc tggcgctgct
    12061 cgcggtgggc gtgctgatgt ttctcgtgct ggtgggattg atcacgctct tccgcgccgt
    12121 gggttagccg gaacgtcgcc cggccggtag gccgggcgac gcgtcagggc ttgtgatttc
    12181 cttccaccca catggtggcc cgacgcacga gttcgcgccc gctcgacacg tccagcttca
    12241 ccttgatgtg ctccaggtgg ctctccacgg tcttcacgct cacgcagagc tgcgtggcga
    12301 tgtcacgcga cgagagaccc gcaccgacca gctcgaacac ctggagttcg cgatccgaaa
    12361 ggttggccac gcccgacttg gcgggcgcgc tgccgccggc cccgcggcgg aggaaatgga
    12421 cggtggtggc gctcgaaagc accgcctgcc cgccactggc ccggcgaatg gcgcggatga
    12481 gcttttcccc cggctccgac ttgttcacgt agcccagggc accggcccga aggatgcgtt
    12541 cggcgtagaa cgcctcgtcg tgcatgctga ggaccagcac cttcacttcg gggaagcggc
    12601 cggtgatgtc cttgaccaga tccaggccgc tctcggtgcc gagcgtcagg tcgacgacgg
    12661 ccacgttcgg cgcgcactcc tggatgactt cgagcgccgt cgagatgctc cccgcctcgc
    12721 cgcacacttc gatgtcggtc tcgttcgcga tcaagtcacg gagcccctgg cggacgatcg
    12781 gatggtcgtc gatcagcacc accttgatgg gacgcctggc cgtggaagtt tccgttttcg
    12841 tcatcatcat gcttgccccc attgaaagag ccggacgacc cggccttatc cgacgccgat
    12901 tcggggcacc gacaggtgaa cgtgtgtccc gtcgaggccc tggatttcca gccggccgcc
    12961 gatcaggcgg gcccgctggt acatcgatcg cattccctgg ccgcggatgg cgtgctcggc
    13021 gatgcccagc ccgtcgtcgt ccacgtccag actgatcctc tgctcgtcct gccacaagcg
    13081 aattcgcacc cggcggcacc gggaatgact tatcgcgttc tgcagggcct cctgtgcgat
    13141 gcggtagagg tggttggtca cgtcgggatc tttcatgtcg atgaccggat cgacctccac
    13201 gacgcaggtc atctgggtgg agctgttcct ctcggccagc catcccagct cgaccagcat
    13261 ttcgcccagg gcattggccc ggtcgggtac gcggaccagg ccgcgggcca cgctctggat
    13321 gtcgacgagc acctggcgga gccccccggc cacgcgctcc agggtctggc gctgcccccc
    13381 gttccccgac tccctccggc tcatcgattc cacagtcagg gcaagccccg ccagctcctg
    13441 gccgaccgcc tcgtggagat cgcgcgtgac ccggtcctgt tcgtacacgg tgagctcgag
    13501 gagctgccgg gccaggtcat cccgctggat cacatgggcc agctgcgtgc acacccccga
    13561 catcgtgtcg agcatgtcgt cgtccggctc gatgacgttg gagaggtaga acttgagaac
    13621 ggccaccggc tccgagccgg ccgtgacggg aaagaccatg atcatgcgca gcccggcctg
    13681 cacggccgcc gcggccatcg gggccggcag gttggcggag agatcgtcgg accagagagg
    13741 gcggctttcg ctgaaggccc gaacggggag ccggtcggag agtggcgccg cctccgcccg
    13801 caccagtcgt tcgagcgaca tgaagcggac ggggtcggtg gcatggacga tgggtggcgt
    13861 gagcccgagc gccgagggaa gcccccacgt ggaacggaag gccaccccga actcccagcc
    13921 gtagtgttcg caggtgcgcc gcagcgtgta ggccagcatt tcctccaccg aggcactgcg
    13981 gctgttgccg gccaccgcaa tatcgcgcag gaacgagagg tcggacggaa ccgtgcccga
    14041 cccgttcccg gaggccccgc gcacggcagc gaggtcgacc cggcatcgga gcaggcggcg
    14101 gcgcgtccgg tcgagttcac cggtcaggcg gtcgatttcg agctgcatgc gggcggagct
    14161 gctgtctgtc atggtgtgcg accgatcggc tgacacagag atctggaagg gcgcggcggg
    14221 gaacgtgtcg agaccccatt ttccggtccg catggttctc ccgacaatcg gggattcccc
    14281 cacgtacgcg ccggcattac ccggagcgcg aatggtgggg ggccttattc cgcccggacc
    14341 cctccagggc gcactctcct ggacatacca cgggactgga tgtcgcgtga gccacacgtc
    14401 cctatgcgac gaatcggcca cggacatccg gttctgggga ccttacttcg cttcccctcg
    14461 ccgcctcgct ccgactctcg ccggcacctc tccatgcatc cgtctggcgc gatcacccgg
    14521 aaacctcttc catccatcga ttccttcctt cgtcgggcca tctcccatgg aggtcggaat
    14581 gacggtgcgc tccggttgaa tcgggaagct tcgtgtcgat ttcgcgggcc cagggggcga
    14641 atttcgacgg cgcgaatcat gcaggccctc cggcttccgt tcacccgcct ggtcgcagga
    14701 aacgattcca cccggaggtc atcaatgaag gtttcgcaca gggggatcct gggacttgcc
    14761 gccgccatcg cgctgggact cctggccggc tcaaccattc ccgcggtcat ggccgtggcc
    14821 ggcaacgaca agaaccggca cgacgacgaa aagaagaacg acaaccggaa ggacaaggac
    14881 aaggatgacg atgatgatga cgacgacgac cggaacggcc cgggctccct ccgggacgtc
    14941 gtgctccccg gaggctacaa gatccgcgaa tttgccggcg gcctgaactt tccgaccggc
    15001 atcacgtggg acagcaaggg aaacgtcttc gtgagcgagg ccggcatcgc cgacacgccg
    15061 gcccgcatcg tccgcatcga tgaggatgcc gacgtcgttc cggtggccaa cggcttcacg
    15121 gcgccggtca ccgacgtcac ctggcacgac ggctggctgt acgtgtcgca cctgggcacg
    15181 atcagccgca tccgccccga cggttccggc cgtgaggacg tggtgaccgg cctccccgcg
    15241 ttcggcgacc actcgaacgc cgacatcatc ttcggcccgg acggccgcat gtacttttcc
    15301 atcggaaccg ccaccaactc cggcgtggtg gggcccgaca attcctgggt gatggatcat
    15361 ccggaagtcc acgacattcc ggccgaaacg atcaccgtgc gggccgagaa ctacacgtca
    15421 ccgaacatca tgacctcgac ccccaatgac gtcaccttga ccggcccctt ccagccgttc
    15481 ggcgaggccg ccgcgctcgg tgaggtggtt cccggcgagg tgaagagcaa cggctcgatc
    15541 ctttccgcca acccggacgg ctccgacctc caggtgtacg cgtggggatt ccggaacgcc
    15601 ttcggcctcg gtttcgatga gtccggccag ctctgggcca cgaacaacgg catggacgag
    15661 cggggcagcc gccccatcga gggtgacctc gacgccttct tccaggtgga gcagggcgac
    15721 tggtacggct ggcctgattt ctcgctcggc cgtccggtga accaggagct gttcgaggtg
    15781 gatggcgtca tccccgagct tctcctcctt tcgcacccgc ccctgtcacc gctggccgac
    15841 gcgttcgcgg tcttcgagga ccatgtctcg gccgacaagt tcgacttctc ctcatcgagc
    15901 aggttccgct acgaggggga tgccttcgtg gccgaaacgg gatcgctccc cacgctgacc
    15961 ggcgccgccg actattacgg ctaccgggtc acgcgggtgg atcaccggac cggcgacgtc
    16021 tccgtctttc tggccaacaa gtcggggaag ccggccttcg tcaccggcga ggacggcatc
    16081 aacaagccga tcgacgtgaa gttcgctccg gacaacgccg acgtgatgtt cgtcgtcgac
    16141 ttcggcgctt tcctgccgac gccggccacc gaagaggagt tcccctcggt cattgaaccg
    16201 gggagcggca ccatctgggt ggtgggtcgg gccagcaccc tcaacgaatt cgcccgtaac
    16261 cagggtgacg atgatgagga tgacgacggg gatgacggcg aggaagaaat cgacggcgcg
    16321 cccggcacgg gcctgctgct gaacacctcg gtgacagccg ggcccacgat caattaccgg
    16381 ctcgagtcgc cgcagatcgt gtcgctccgg gtgttcgacg tgacgggtcg ggtggtgcgc
    16441 acgctggtcg aggagccaca ggtggccggc gaccacgtgg tggcatggga cggccaggac
    16501 gaccagttcg ccgccgtccc gagcggcgtc tatttctatc gcatcgaggc gggagccgat
    16561 cgacagagcg gaaaggtcct gatcacggcc ccgtaattcc tagcgtgacg atatggcccg
    16621 ggggatgccc agccgggccc ggagggaggc gctgactcgc ttgcggatca gcgcctcttc
    16681 cgcaaggcgt tcgcgctcgg gaggcagggc cgccagccag cgcgtctcac ccccctcctt
    16741 caccaccacc gggatgccgt cctcgtagac gatgcggttt ccggccaggg ccgccacgcg
    16801 atcctccggg gagaggatgc cggtcaggtt caggggatcc gctgccgaga gagccgtcaa
    16861 ttgtccctgc ggcccggccc gccgcacggc gcgaagccgg gtgacggcgt cgggaagggc
    16921 gaactgctcg ccggcgatgc cggagacgaa tcgaccaccg cgcacctcgc cacgggcctc
    16981 gagccgccag tacgtgcgga gcaactcgcg ccacggcacg ctgaacgcct cgcgcgcgag
    17041 gagtttccgg aacatcacgc cgtagcgatt gaggagcgtt cgtgccaggt gctcaacggc
    17101 ggcttcgcgg ccggtattgg tcgccccgtg tcccgggtcg gcggaatcgg gccccccgga
    17161 gctgccggcc aatcgcgccc atcgcccggc cgattccacg ccgaaggcga tgctcctgtg
    17221 acgccgcctc gaggcaccgg cgatgggagg ccggcgcgac tccggcgtga tgagcgcccg
    17281 gaggccggtg aagccgtcgg cggtgacgag tccgagcgcg gccagttccg ccagcgctcc
    17341 ttccacctgg gtgccgagaa gccccgtgcg ggtcaccagg tcgtggaaga acatggcgcc
    17401 ccccgcgtcg agcacggaaa ggacgagcgc cgcgttggcg ccgcggggcg tccgctccac
    17461 gtcaccggca agggcgtgcc aggccccgag gtgttcacgg cgcagcagtg cgatgggggt
    17521 cgatcggagc ggtcccgaga tcgctccccg aagcgacggc gaggggggcg acacgcgggc
    17581 ccacatcacc cgccccatga gtgagagcga gtcgaggagc tgtggatcgt aatcaaccga
    17641 gcgggcggac agcacgtccg tctcccacgc gccggccggc acctcgaacc cctcgagctg
    17701 ccccagcacc gccgccagtc cctcgaggcc ggcggtgcgg tgatcgggcg tgacgtgctg
    17761 ccactcgaac aggaatgagc tgaactgcgc gggggtgacc ggttcgatct cggagcggag
    17821 ccggctgatc gtgtagcgat ggatgcgcgc caggaggcgg cgctcgcacc actcgagatc
    17881 ctccacgccg ggcgtgaagt gaccgcgcag gacgacgccg tccgcttcga tcgcgaggag
    17941 cgcggcgtcc acggcggcgc gcgggagcct tatcatggcg gccagctgtt cggcggtgac
    18001 cgggccggcg atctcgagcc gcccgcgcac gcgttccacg tcggagagct cccgccgctc
    18061 ggccgccagc cagagatccc cctgccgggc cgtgcgcccg gccgcctcga gcctgtcgcg
    18121 ccactcgggc cagcgggcca tctcctcgtc ggtgagcgtg ccggacacga gcagcgcgtc
    18181 gtgcagctcg tcaggatcgc
//
LOCUS       DASVOD010000313         2490 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_74380, whole genome shotgun sequence.
ACCESSION   DASVOD010000313 DASVOD010000000
VERSION     DASVOD010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74380"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VF720_07055"
     CDS             <1..819
                     /locus_tag="VF720_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879151.1"
                     /translation="DCSQFKSSKGTKDCKTRVENMNKIIRNVDSFTRSNNVKLAEDEL
                     AKAEKAADPAAKRAAYASAIGFFEAALQQDPKLNGVRFDLANTHFATAESFAAENNKS
                     EANTSYIKAAEIFTTLADNDSVEAETKNLALYNGASALYSAEEFAKASPLFRRYIDVA
                     PREVAPWRLAGSCIIENRAEAIGYLSMGNALSDKAVTTPVDESVGTVRNLYAASAAAK
                     ALAELGNPEEVRTFTDEKNGGQVVTTWIWWSKGQARHYLNGDELGKVSFRAAAG"
     gene            complement(926..2053)
                     /locus_tag="VF720_07060"
     CDS             complement(926..2053)
                     /locus_tag="VF720_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7879152.1"
                     /translation="MSTYSWRRWLMAGGVATALASLTAVVTTAKDHAGHEKHEKTTGY
                     YYISDDGDDDGDARSQGYLGVSVQRLRSRLREAMDIPSDVDGLIITNVHDDTPADDAG
                     LRNNDVILRVNKLEVGDEDEFTDMIREFKPEAKVTVSIWRDGATRDVPVTLARRPRTA
                     VYSWGSDAGPHAYSWHGNGDMAAVPRAPRPPRAPRTPMAPRTPIAPITPLSPHSFGMF
                     NPAGGIGRARLGIEIQDLNEEIGSYFGAADGEGVLVWRVHEDGPADKAGLKAGDVIVE
                     IEGHDIDDVSELREEMADHDDGESVAVTWLRQGKSMSAGITLEEGDTPSMYWFSDTPG
                     RGAGPGMSRSLDRGMETMQREMEKLQRQMEELQEKMERLKD"
     gene            2298..>2490
                     /locus_tag="VF720_07065"
     CDS             2298..>2490
                     /locus_tag="VF720_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX7879153.1"
                     /translation="MPRGIRDPLVLGAMRAVPRHHFVPDALRANAYDDNPLPIGHEQT
                     ISQPYIVAYMTEALELGGGE"
BASE COUNT          417 a          833 c          813 g          427 t
ORIGIN      
        1 gattgcagcc agttcaagag cagcaaggga accaaggact gcaagacccg cgtcgaaaac
       61 atgaacaaga tcatccgtaa cgtcgacagc ttcacccgca gcaacaacgt gaagctggcc
      121 gaggacgagc tcgccaaggc ggaaaaggcg gccgaccctg ccgccaagcg ggccgcgtat
      181 gcctcggcaa tcggtttctt cgaggcggcc ctccagcagg atccgaagct caacggcgtc
      241 cggttcgacc tggccaacac ccatttcgcc accgccgaga gctttgccgc cgagaacaac
      301 aagtcggagg ccaacaccag ctacatcaag gcggcggaga tcttcacgac gctggccgac
      361 aacgactcgg tcgaggccga gacgaagaac ctcgcgctct acaacggggc ttcggcgctc
      421 tacagtgccg aggagttcgc caaggccagc ccgctcttcc gtcgttacat cgacgtggct
      481 ccgcgcgaag tggcgccgtg gcgactggcc ggaagctgca tcatcgagaa ccgcgcggaa
      541 gcgatcggct acctctcgat ggggaatgcg ctcagcgaca aggcggtgac gacgccggtc
      601 gacgaatccg tgggaacggt caggaatctc tacgccgcct ccgcggccgc caaggcgctg
      661 gccgagctgg ggaatcccga ggaagtgcgc accttcaccg acgaaaagaa cggcggccag
      721 gtcgtgacga cgtggatctg gtggtcgaag ggacaggccc gccattacct gaacggtgac
      781 gaactcggca aggtcagctt ccgggcggcg gcgggataag ctcaccggtc atatacattc
      841 gacgcatcaa acgaaaggcc ccggcggttt ccgccgggac cttttcgtct tgcggtgtgt
      901 gtgcggactc tcttccggtg ttcccctagt ccttcagccg ctccatcttc tcctggagct
      961 cttccatctg gcgctggagc ttctccatct cccgctgcat cgtctccatc ccgcggtcga
     1021 ggctgcggga catccccggc ccggcgcctc ggcccggagt atcggagaac cagtacatgc
     1081 tcggagtatc cccctcttcg agcgtgatgc cggccgacat cgacttcccc tggcggagcc
     1141 atgtgacggc aacgctctcg ccgtcgtcgt ggtcggccat ctcctcccgg agctcgctca
     1201 cgtcatcgat gtcatgcccc tcgatctcga cgatgacgtc tcccgccttg agtccggcct
     1261 tgtcggccgg gccgtcttcg tgcacacgcc agacgagcac gccctcgcca tccgctgcgc
     1321 cgaagtacga gccgatttcc tcgttcaggt cctggatctc gatgcccagc cgcgcgcggc
     1381 cgatcccgcc ggcgggattg aacatgccga aggaatgggg agacaggggg gtgatcggtg
     1441 cgatcggggt ccgcggagcc atgggcgtcc ggggcgcgcg gggcggacgc ggagcacgag
     1501 gcacggccgc catgtcgcca ttgccgtgcc acgagtaggc gtggggtccc gcgtcggacc
     1561 cccagctgta gacggccgtc cggggacggc gggccagcgt gaccggcaca tcgcgcgtgg
     1621 cgccgtctcg ccagatcgag accgtcacct tcgcctccgg tttgaactcg cggatcatgt
     1681 cggtgaactc gtcttcgtcg ccgacctcca gcttgtttac gcggaggatg acgtcgttgt
     1741 tgcggaggcc cgcgtcatcg gccggggtgt cgtcatgcac gttggtgatg atgaggccgt
     1801 cgacatccga tgggatgtcc atggcttcgc ggaggcggct gcgcagtcgc tgcaccgaga
     1861 cgccgaggta tccctggctg cgtgcgtcgc cgtcgtcatc accatcgtcg gagatgtagt
     1921 agtagcccgt ggtcttctcg tgcttctcgt ggcctgcgtg gtccttggcg gtcgtcacga
     1981 cagcggtgag ggaggcgagg gcggtggcca cgccgccggc catgagccag cgccgccacg
     2041 aataggtcga cattccggat actcctttcg agggggtggg acggttcggg tcgctgcctg
     2101 gtgggcggct tgccgccgct tagacgccgc cggcttgccg atagttcaat ggattcgacc
     2161 tgccgcagcc ccgcccgggg actgggtttg acccgcttgc gggatcgggt caatactcac
     2221 cggacggggc ggttccctgc gccgcccgac cggaggaagc acccttgagc gccccctcgt
     2281 tccttcacac ccagctgatg ccgcgcggca tccgggatcc cctggtcctc ggtgccatga
     2341 gggcggttcc ccggcaccac ttcgtccccg acgcgctgcg cgccaacgcc tacgacgaca
     2401 atccgctccc gatcgggcac gagcagacaa tcagccagcc ctacatcgtg gcctacatga
     2461 ccgaggcgct ggagcttggc gggggagagc
//
LOCUS       DASVOD010000314         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_804114, whole genome shotgun sequence.
ACCESSION   DASVOD010000314 DASVOD010000000
VERSION     DASVOD010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804114"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..611)
                     /locus_tag="VF720_07070"
     CDS             complement(36..611)
                     /locus_tag="VF720_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX7879154.1"
                     /translation="MDLVNALLGAFGLAVAAGLNAWIPVVLVGAAARWLPGGWLELSP
                     KFAFLSSDWFLIFAGVMLLIELAADKIPVVDHVNDIIQSVVRPLAGAVLFAAGSGVID
                     GLDGRIALVLGFLTAGSIHGVKSAFRPLVTATTGGLGNPVVSFVEDVLSFLATLLAIL
                     IPLALIAFFVGVPILVVRWWGKRRARAPVRT"
     gene            693..2117
                     /locus_tag="VF720_07075"
     CDS             693..2117
                     /locus_tag="VF720_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7879155.1"
                     /translation="MTMARAANPILDSPHLSRVTLSLAWPIIVSRLLHTFFHLANVAW
                     VGRLGPEAIGAVTTSIYLFWTWYALAGMITLGATSVVARHVGAGRPDEAARVAGQGVG
                     AILVLACVMTALGFVGAGPLFGIISDDPDVRRIGADYVRIVSLASVPTYIILIGEAIY
                     RGSGDTRTPMRVLVATLAVHVVLDPFLILGVGPFPRLGVHGAALATLITQSLGATIYL
                     VLALCGRWPFSVAAMFKGFRADGQVIRAMAGIGAPISAIGLLFSSVYLVLAKIAAGYG
                     TPILAALGIVNRLESFSFLTSEGFGVAATTTTGQNIGAGQLDRADRLARRARTWSTAL
                     TFVCGVIFLAVPEQILMLFTDDPSVIERGALFLRIVALAQPFQGMEIALEGALAGAGD
                     TRPAMLIAVPLSLVRIPIILWLAGPMGMGAVAIWWTITVTSILRGLLVEAWFRSGRWR
                     RRHEAPGAGQVTPGNPSPAVGEGR"
     gene            2158..2517
                     /locus_tag="VF720_07080"
     CDS             2158..2517
                     /locus_tag="VF720_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879156.1"
                     /translation="MKFLIYGFLLAGAIFLANMFYGPIIKNHMLEGKMINLVNESRLK
                     DDFYLMRDLMSFVEENNIPVKQEQFVLQRPQPKEVILDATYKVDCKFYFLEKHYVFKP
                     TTRNESAHGLLPDFAAY"
     gene            2552..>3603
                     /locus_tag="VF720_07085"
     CDS             2552..>3603
                     /locus_tag="VF720_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7879157.1"
                     /translation="MTTRRPPRGRTDSPGLSPDRGEGPVLTAGWPPGGGAGSPVIVGS
                     NARMQRIYNLIARIAPTDSSVLIVGESGTGKELVARSIHLQSRRADQPFLAINCGALP
                     ENLLESELFGHVRGAFTGAFTDKKGLFEQADHGTLFLDEVGELAPQSQVKLLRVLQEN
                     EIRRVGGNTNLKVDVRILAATNRDLRQAMQHGRFREDLYYRLNVFQIDLPPLRERGED
                     VLLLAHYFLDLYARRLGKSIRGFDGDASYQLTHYPWPGNVRELENVVQRATALAEGDV
                     IRVDDLPRHLREARMPLLDAPTGGLPSLPEGLRLDELEKLYMEQTLGRNAGNVSRSAR
                     QLGISRSTMWRKMKQYG"
BASE COUNT          597 a         1271 c         1158 g          577 t
ORIGIN      
        1 accggttgta gccggcgctc gaccggtcga gcgcgttacg tgcggacggg agctcgtgcc
       61 cgcctctttc cccaccagcg cacgacgagg atcggtacgc ccacgaagaa ggcgatcagc
      121 gccagcggga tgaggatggc cagcagcgtg gccaggaacg acagcacatc ctccacgaac
      181 gacaccaccg gatttcccag cccgccggtc gtggccgtca ccaacggccg gaaggcgctc
      241 ttcacgccgt ggatcgagcc ggcggtcagg aagccgagca cgagtgcgat acggccatcc
      301 aggccgtcga tgaccccgct gccggccgcg aacaggacgg cccctgcaag ggggcgtacc
      361 acggactgga tgatgtcgtt gacatggtcg acgaccggga tcttgtcggc cgccagttcg
      421 atcagcaaca tgacgcccgc gaagatcagg aaccagtcgc tcgagaggaa ggcgaacttc
      481 ggcgacagct cgagccaccc accggggagc cagcgggcgg ccgcacccac cagcaccacg
      541 ggaatccacg cgttgagccc cgcggcaacc gccaggccga aggcaccaag gagagcgttg
      601 accaggtcca tcaccagtcc tccggaaact ccaggaccgc cgaccggccg gcggtgcggt
      661 cagtgtacgt ccaccgggcg cggcggttgc ggatgacgat ggcccgcgcc gccaatccca
      721 tcctggacag cccgcacctg tcgcgggtca cgctctcact ggcctggccg atcatcgtct
      781 cgcgcctgct ccatacgttc ttccacctgg ccaacgtggc ctgggtggga cggctcgggc
      841 cggaggccat tggcgcggtc accaccagca tctacctctt ctggacctgg tacgcgctgg
      901 ccggaatgat cacgctcggc gccacgtcgg tggtggcacg gcacgtgggc gccggtcggc
      961 cggacgaggc ggcgcgggtg gccggccagg gggtcggggc catcctcgtc ctcgcgtgcg
     1021 tgatgacggc gctcggtttc gtgggggccg gacccctctt cgggatcatc tccgacgacc
     1081 cggacgtccg ccggatcggc gccgattacg tgaggatcgt gtcgctggcg tcggtgccca
     1141 cctacatcat cctcatcggc gaggccatct accggggaag cggcgatacg cggacgccga
     1201 tgcgggtgct cgtggccacg ctggccgtgc acgtggtcct cgatccgttc ctcatcctcg
     1261 gcgtcggccc ctttcccagg ctcggcgtgc acggggcagc gctggccaca ctcatcacgc
     1321 agagcctcgg cgcaacgatc tacctcgtgc tggccctgtg cggtcgctgg cctttctccg
     1381 tggcggccat gttcaagggc tttcgcgccg atgggcaagt gatccgcgcc atggccggca
     1441 tcggtgcgcc gatctcggcg atcgggctcc tcttttcgtc cgtgtacctc gtgctcgcga
     1501 agatcgccgc cggctacgga acgccgatcc tggcggcgct cggcatcgtc aaccggctcg
     1561 agtcgttctc gttcctcacc tccgaggggt tcggcgtggc ggccaccacc accaccggcc
     1621 agaacatcgg ggccggccag ctcgaccgcg cggatcgact ggcgcgccgg gcccgcacct
     1681 ggtccaccgc actcacgttc gtatgcggcg tcatcttcct cgcggtgccg gagcaaatcc
     1741 tcatgctctt caccgacgac cccagcgtga tcgagcgcgg cgcgctgttc ctccggatcg
     1801 tggccctcgc ccagcccttc cagggaatgg agatcgccct ggagggtgcc ctggccggcg
     1861 ccggcgacac ccgcccggcc atgctgatcg ccgtgcctct ctccctggtc cggatcccga
     1921 tcatcctctg gctggccggg ccgatgggaa tgggggccgt ggccatctgg tggacaatca
     1981 ccgtgacctc gatcctgcgc ggcctcctcg tggaggcctg gttccggtcg gggcggtggc
     2041 gccggcgcca cgaggccccg ggagcgggtc aggtgacccc gggaaatccc tcacccgcgg
     2101 ttggggaagg ccgataactc cggggagatc tcccagagga tcaaccggag gcgtgtcatg
     2161 aaattcctga tctacggctt tctcctggct ggcgcaatct ttctggccaa catgttttac
     2221 ggcccgatca tcaagaacca catgctcgag gggaagatga tcaacctggt caacgagtcg
     2281 cggctgaagg acgatttcta cctcatgcgc gacctgatgt cgttcgtgga ggagaacaac
     2341 atcccggtca agcaggaaca atttgtcctg cagcggcctc agccgaagga agtcattctc
     2401 gacgccacgt acaaggtgga ctgcaagttt tacttcctcg agaagcacta cgtcttcaag
     2461 cccaccaccc gcaacgaatc ggcccacgga cttctgcccg acttcgccgc gtattgaccc
     2521 cggccccgcg ggggtgtggc agaatgaacg ggtgaccacc cgacgccctc cccgcggccg
     2581 aaccgactcg ccgggcctct cgcccgaccg gggcgagggc cccgtgctca cggccggatg
     2641 gccgccaggc ggcggcgccg gcagtccggt gatcgtgggc agcaacgcgc ggatgcagcg
     2701 catctacaac ctgatcgcgc gcatcgcccc taccgactcg agtgtgttga tcgtcgggga
     2761 aagcggcacc ggcaaggaac tggtggcccg ctccatccac ctgcagagtc ggcgggccga
     2821 ccagcccttc ctggccatca actgcggtgc gctgcccgag aacctcctcg agagtgaact
     2881 cttcggacac gtgcgcggcg cgttcaccgg cgcgttcacc gacaagaagg gactcttcga
     2941 gcaggctgat cacggcactc tttttctgga cgaggtcggc gagctggccc cgcagagcca
     3001 ggtgaagctc ctgcgcgtcc tccaggaaaa cgagatccgc cgcgtcggcg gcaacaccaa
     3061 cctcaaggtg gacgtgcgca tcctggccgc cacgaatcgc gacctccggc aagcgatgca
     3121 gcacggccgg ttccgcgagg acctctacta ccggctcaac gtcttccaga tcgacctccc
     3181 cccgttgcgc gaacggggcg aggacgtgct cctccttgcg cactacttcc ttgatctcta
     3241 cgcccggcgc ctgggcaaat cgatccgcgg cttcgacggc gacgccagct accagctcac
     3301 gcactacccg tggccgggaa acgtccgcga actcgagaac gtggtgcagc gcgccacggc
     3361 cctggccgag ggcgacgtga tccgtgtgga tgacctcccc cgccacctcc gcgaagcccg
     3421 gatgcccctg ctcgacgccc cgaccggtgg cctgccttcc ctgcccgagg gtctccggct
     3481 ggacgagctc gagaagctat atatggagca gaccctggga aggaacgccg gcaacgtcag
     3541 ccgcagcgcg cgtcagcttg gcatctctcg ctcgacaatg tggcgcaaga tgaaacagta
     3601 tgg
//
LOCUS       DASVOD010000315         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_961498, whole genome shotgun sequence.
ACCESSION   DASVOD010000315 DASVOD010000000
VERSION     DASVOD010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961498"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1680)
                     /locus_tag="VF720_07090"
     CDS             complement(<1..>1680)
                     /locus_tag="VF720_07090"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879158.1"
                     /translation="NHEHFFPGNPVDVRAEGKIAKKPWRAGGKFGPDSPGEFARILAE
                     MKVPGDREASEYRPGYQHAELEAALVRSGRRRRDVFDARGVSQTAVALPWVERGPGNV
                     AGRARAIVVDPDDAGHNTWYIGTAGGGVWKTTNAGANWSWITPTFPVLSVSALAMAAS
                     NHDILYAGTGESFFNVDVMNGNGILKSTDRGVSWTPLAATINNPQFNNVSRLLVDPTN
                     PNLVLASTTQGRYTVTVAQRSSIMRSTDGGANWTEVYFRVDTGSGSLVKKILQLVADP
                     TDFNILYGTVDEAGIIKSTNRGLTWSTINTGITDLSGRFELAVCPTDHNRLFAAAEGA
                     SHSELWLSTNGGTSWSETIEAGTEPSWLGNQGWYDNAIVPHPTDPNIVYVGGVNLARI
                     NLSGTSRTTTFIASGMHPDHHGLMTINAPGGWRILNTNDGGVTLSTLQDNGFNSLIDG
                     MVTTQFYGADKRPGASAYAGGTQDNGTWQSSEGTTATTPWSHVIGGDGYEVSWNFDDP
                     SKIIGAYQYNGIQRSLDGGMTWNPANISTSPAGLIDNGAGNAPFITKIAKSN"
BASE COUNT          234 a          536 c          590 g          320 t
ORIGIN      
        1 gttcgacttg gcgatcttgg tgatgaaggg ggcattgccc gcgccgttgt cgatcaaccc
       61 ggccgggctg gtcgagatgt tggccgggtt ccaggtcatg ccgccgtcca gcgagcgctg
      121 gatgccgttg tactggtagg cgccgatgat cttcgacggg tcgtcgaaat tccacgacac
      181 ctcgtagccg tcaccgccga tcacgtggct ccagggcgtg gtggccgtcg tgccctcgga
      241 agactgccag gtgccgttgt cctgcgtgcc gccggcgtag gcgctggcgc ccgggcgctt
      301 gtcggccccg tagaactgcg tcgtcaccat gccgtcgatg agcgagttga agccgttgtc
      361 ctgcaatgtg gacaacgtca cgccgccgtc gttcgtgttg aggatgcgcc agccgcccgg
      421 tgcgttgatc gtcatgaggc cgtggtggtc cggatgcatg cccgacgcga tgaacgtggt
      481 ggtgcgcgag gtgcccgaca ggttgatgcg cgccaggttg acgcccccca cgtacacgat
      541 gttcgggtcg gtcgggtgcg gcacgatcgc gttgtcgtac cacccctggt tgccgagcca
      601 cgagggctcc gtcccggcct cgatcgtttc cgaccaggag gtgccgccgt tggtcgagag
      661 ccagagctcc gaatggctgg caccttcggc cgccgcgaag aggcggttgt ggtcggtggg
      721 gcacacggcc agctcgaagc gccccgacag gtcggtgatg ccggtgttga tcgtgctcca
      781 cgtgagcccg cggttggtcg acttgatgat gccggcctcg tcgaccgtgc cgtacaggat
      841 gttgaagtcg gtcgggtcgg ccacgagctg caggatcttc ttgacgaggc ttcccgaacc
      901 ggtgtccacg cggaagtaca cctcggtcca gttcgcgccg ccgtcggtcg agcgcatgat
      961 gctcgagcgc tgggccaccg tcaccgtata gcgcccctgc gttgtggagg ccagtaccag
     1021 gttgggattg gtcggatcga ccaggaggcg cgacacgttg ttgaactgcg ggttgttgat
     1081 cgtggcggcc agcggcgtcc agctcacgcc acggtcggtc gacttcagga tgccgttgcc
     1141 gttcatcacg tcgacgttga agaagctctc gccggtgccg gcgtacagga tgtcgtggtt
     1201 tgatgcggcc atggccagcg cggacactga cagcacgggg aacgtcggcg tgatccagct
     1261 ccagttggcg ccggcgttcg tggtcttcca gacaccgccg ccggcggttc cgatatacca
     1321 ggtgttgtgc ccggcgtcgt ccgggtcgac gacgatcgct cgggcgcgtc ccgccacgtt
     1381 tccggggccc cgttccaccc acgggagcgc caccgccgtc tgcgacacgc cgcgcgcgtc
     1441 gaagacgtca cggcgccgcc ggccggaacg gaccagggcc gcctcgagtt ccgcgtgctg
     1501 gtagccggga cggtattccg aggcctcccg gtctcccggc accttcatct cggcaaggat
     1561 gcgggcgaac tcccccgggc tgtcgggtcc gaacttgccg ccggcccgcc agggcttctt
     1621 ggcgattttg ccctcggcac ggacgtcgac cgggttaccg ggaaaaaagt gctcgtgatt
//
LOCUS       DASVOD010000316         6677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_126741, whole genome shotgun sequence.
ACCESSION   DASVOD010000316 DASVOD010000000
VERSION     DASVOD010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126741"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="VF720_07095"
     CDS             <1..693
                     /locus_tag="VF720_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879159.1"
                     /translation="GWLPMIMAIPGLAGPAGLLAAGLAQFVFLPLTFIGARVRYIVPF
                     LPILWILSVVAIETLTSRGIRAAAAALLLLSIAWGAWNGRDLYTMNEDGYFPELVEAG
                     KFLKELSTSATVVYDRKPYTAFYAGAVGRFTPAGDYNAILDEVRAKGGDYLVVNEWVA
                     AHFRPALTPLARDANVIDAETRLVPIYFDVASPGHRTLIYRVVRPGGPAAMPGEDEVR
                     AGILAYLSSPPR"
     gene            complement(700..2112)
                     /locus_tag="VF720_07100"
     CDS             complement(700..2112)
                     /locus_tag="VF720_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7879160.1"
                     /translation="MPTLPDWLTQILAIALLLGVIALVIRSLPKVDLGHSPEFLARRF
                     QNWFPLGLTYAFLYMGRYNLTVSKNALGDLMTKADFGTIFFWGTLTYGTAFVINGPLT
                     DKWGGRRTILIAAAGSSICNILMGIVTWLRLTTAWQPPGGSLVPIFSLLYAANMYFQS
                     FGAVSIVKVNASWFHVRERGILGGLFGILISLGLYFAYDWSGFIVKHAATYWVFFVPA
                     VILIAFFIIDWFVIRDTPGEAGQKDFDVGDASSGDTGPPAGVWEVARRMLTNPVIVII
                     ALVEFCSGFLRNAVMQWYTIFAKEVGITGQFVPANWGLLLCVAGITGGMLAGIISDHV
                     FGSRRGPVAALLYAGLVVGAALVYFMLASPMLGFVAILMSLCFIGVHGMLSGTASADF
                     GGRKNAGVAVGIIDGLVYLGTAVQSILLGKVLPSGEAAKDPHNWTNWPLAMLPVAVIG
                     FLLCLKLWNARPNAKGAGAH"
     gene            complement(2352..2828)
                     /locus_tag="VF720_07105"
     CDS             complement(2352..2828)
                     /locus_tag="VF720_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879161.1"
                     /translation="MFKKALISSAALALLLTLPGSSRANSDGFGLGFIAGEPTGVSGK
                     LWMGRTTALDMAVAWSSAGDDDDLHLQADHVWHDFGLLSVDEGRLPVYYGIGARVRFD
                     DGPGDDGVGIRFPVGLAYLFQGNKVDIFGEVVPTLEVTPDSDFDVEGGIGLRYFFH"
     gene            3162..3773
                     /locus_tag="VF720_07110"
     CDS             3162..3773
                     /locus_tag="VF720_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879162.1"
                     /translation="MSPIRRLSAVLVVLAFVGCAGGTEVDSIWKDPTAVGPKGNLLIV
                     GLSDNQTSRRMFEDALTSTLTAAGNQASQSYPVIPSGREADKQAMTGMLREKGFDAIL
                     TARAVSEDKEKIYNPEPEYFTPPSYYYSYWDFYSPATQAVSAPGYYTEETWVRVETNL
                     YDADGKLIWTAATSTLKNGDMREEIHSLCATLTKQLEKSGLIK"
     gene            3825..6494
                     /locus_tag="VF720_07115"
     CDS             3825..6494
                     /locus_tag="VF720_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7879163.1"
                     /translation="MPLTAGSRLGTYEILGPLGAGGMGEVFRARDSRLGRDVAIKVLP
                     DAFAQDPERLARFEREARLLASLTHANIAGIHGLEVVDGHRHLILEFVDGETLANRLA
                     RGPLPLDEALGIASQIAGALDAAHEAGIVHRDLKPGNVMLTAAGDVKVLDFGLARGGP
                     ASSSDPSLSASPTMTYAATMAGVILGTAAYMSPEQARGKAVDRRTDIWSFGCVLYECL
                     TARVLFAGETVSDTIAKILEREPDWSALPERTPPRVRELLRRCLEKDPKKRQRDAGDI
                     RLELEEILAGGMSSSAASGSAGAAPPRGRPARLPWVIAGVSLVLALVSLAGLFRPPPP
                     SPPPLRTSITFPPGVGLFEWEASDLAVSPDGSLLAWAGNDSTEKSGVWVRPLDGLEAR
                     LVARLDAGPGLPFWSPDSRRVAYFADGKLRVVGLDGNPPQVICDALDGRGGTWNADGV
                     ILFTPTSAGPIHRVAAAGGKSEPILELDAAAGETGQRYPRFLPDGRHFTFTSVPARDG
                     GLLSYVGRLGSNERHFLASATTGAILAAPGQVVFARDNNLMVQRCNPTNWKLIGDPET
                     IAFGLGRYRTIGRPAAAATASLLIFPSFKTRDYRLAWIGRDDGIRQPLEVPSGFWSGV
                     YPSPDGRKAVAFRLSDDGKLRLWMIDTVRGTGAQMPEGDDTFGPAAWTPDGREFYYGK
                     RIGTRQDFYVGSAAGSEPARPFLEGGMLFKGSPDVSPDGRYLLFAQLEATTRRDIWYA
                     PIAGGDPVRLIGGPAEEAGPAFSPDGRWVVYQAQESGRPELWVAPFPSGKGAMQITTA
                     GGTGPTWVAGTWEILYVDMSGHVTSIPVTPGPSPVFGTPTPIRALAGLEDRLQTAKAT
                     RDGRRFLVSITDQESQDPTLTVISNWQGLLEKP"
BASE COUNT         1153 a         2247 c         2226 g         1051 t
ORIGIN      
        1 ggctggctgc cgatgatcat ggccattccg ggcctggccg gtccggccgg gctcctcgcg
       61 gccgggctcg cgcagttcgt cttcctgccg ctcacgttca tcggggcgcg cgtgcgttac
      121 atcgtgccgt tcctgccgat tctctggatt ctgtcagttg tggcgattga aacactcacg
      181 agtcgcggga ttcgcgcggc ggcggccgcg ctcctcctcc tgtcgatcgc ctggggcgca
      241 tggaacggcc gcgatctcta cacgatgaac gaagacggct acttcccgga gctggtggag
      301 gccgggaaat ttctcaagga attgagcacg tccgccacgg tcgtctacga ccgcaaaccc
      361 tacaccgcct tctatgcggg tgccgtcggg cgcttcacgc ccgccggcga ttacaacgcc
      421 atcctcgacg aggtgcgcgc caagggtggc gactacctgg tcgtgaacga gtgggtggcc
      481 gcgcatttcc ggccggcgct cacgcctctg gcgcgggatg ccaacgtcat cgacgccgag
      541 acgcgcctgg tgccgatcta cttcgacgtg gcttcacccg gacaccgcac gctcatctac
      601 cgcgtggtgc gcccgggtgg ccccgccgcc atgccaggag aggacgaggt gcgggcgggg
      661 attctcgcgt acctgtcgtc accgccgcgt tgagcgacgt caatgcgcgc cggcgccctt
      721 ggcgttgggc cgcgcattcc acagcttcag gcacaacagg aagccgataa ccgccaccgg
      781 cagcattgcc aacggccagt tggtccagtt gtggggatcc ttggcggcct cgccactcgg
      841 gagcaccttg ccaagcagga tgctctgcac ggccgtaccc aggtagacga gcccgtcgat
      901 gatcccgacc gccacgccgg cgttcttgcg gccgccgaag tcggcgctgg cggtgccgga
      961 cagcatgccg tgcacgccga tgaagcagag ggacatcagg atggccacga agccaagcat
     1021 ggggctggcc agcatgaagt agacgagcgc cgcgccgacc acgaggccgg cgtagaggag
     1081 cgcggccacg ggtccgcggc gtgagccgaa gacgtggtcg gagatgatgc cggccagcat
     1141 gccgccggtg atgccggcca cgcagaggag gaggccccag tttgcgggca cgaactggcc
     1201 ggtgatcccg acttccttgg cgaagatggt gtaccactgc atgaccgcgt tccggaggaa
     1261 gccgctgcag aactccacca gggcgatgat cacgatgacg gggttggtga gcatgcggcg
     1321 ggccacttcc cacacgccgg cgggcggacc ggtgtcgccg gaggaggcgt cgcccacgtc
     1381 gaagtccttc tggccggcct cgccgggcgt atcgcggatg acgaaccagt cgatgatgaa
     1441 gaaggcaatc aggatcacgg ccggcacgaa gaacacccag tacgtggcgg cgtgcttcac
     1501 gatgaatccc gaccagtcgt aggcgaagta gaggccgagc gagatcagga tgccgaagag
     1561 gccgccgagg atgccgcgct cgcgcacgtg aaaccaggag gcgttcacct tcacgatcga
     1621 caccgcgccg aagctctgga agtacatgtt ggccgcgtac agcagcgaga agatcggaac
     1681 caggctgccg cccggcggct gccacgccgt ggtgaggcgc agccacgtga cgattcccat
     1741 gagaatgttg cagatggagc tgccggcggc ggcaatcagg atcgtgcgcc gtccacccca
     1801 cttgtcggtg agcggaccgt tgatcacgaa cgcggtgccg taggtaagcg tgccccagaa
     1861 aaagatggtg ccgaagtcgg ccttcgtcat caggtcgccc agcgcattct tgctgacggt
     1921 caggttgtaa cgacccatgt agagaaacgc gtacgtgagc cccagtggaa accaattctg
     1981 gaagcgccgc gccaggaatt cgggcgaatg ccccaggtcc accttcggga gacttcgtat
     2041 gacgagggcg atgacgccaa gcaggagggc aatggcaagg atctgggtga gccagtcggg
     2101 gagtgtcggc atcgatcacc tcgtttagcg gcgtgcgacg tgacgggcgg gccggaaagc
     2161 gtcggaacgg taatcgaagg ccgaaatgag ggcaaggcga tagccaacac cttgtggcgc
     2221 gggtcaaaaa aaggacgccg acggctcatg gatatgggag ccgtcggacg tcaaggcgcc
     2281 gggagtgtac gtcgcacggg tcggcgcctt caccgcgcga gttgtcctgg tccgccagga
     2341 gcggagcagg actagtggaa gaagtagcgt agtccgatcc cgccctcgac atcgaaatcg
     2401 ctgtcggggg taacttcgag cgtcggtact acttctccga agatatcaac cttgttgccc
     2461 tggaagagat aggcaaggcc caccgggaaa cggatgccaa cgccgtcatc gccgggacca
     2521 tcatcgaaac gaacgcgggc gccgatgccg tagtagacgg gcaggcgtcc ttcgtccacc
     2581 gagagaaggc cgaagtcgtg ccacacgtgg tcggcctgaa ggtgaagatc gtcgtcgtcg
     2641 ccggcggatg accacgccac ggccatgtcg agcgccgtcg tgcgtcccat ccagagcttt
     2701 ccactcacgc cggtcggctc accggcgatg aatccaagac cgaagccgtc gctgttggcc
     2761 cgggagctcc ccgggagcgt cagcaggaga gcgagggcgg cactcgatat cagagccttc
     2821 ttgaacatca ttacctccga gtcgggtgtc ttccgggtcg gcccgtgagg ccggtcactt
     2881 ccttgagggg atgcaccttg tgccgccccg cagctgccat cactcatggc aagcgggatg
     2941 ccatccgact catatgccag cacatcaaca ggttgcgcaa aacgcatggg gaggccaccg
     3001 gcgtcgtttc ggggcgaacg tgtcaaaccg acacggtaag actttcgcga tgtggagcgc
     3061 gatctccgcg tgaagcatct gatattctgc gcgccgcgca tggacggcca tgacgcgctg
     3121 cgctcatttc cgacgacacg aacctcaatg aaagggtggt catgtccccg attcgccgtt
     3181 tgtccgccgt gctcgtcgtc ctggccttcg tcggttgcgc cggcggcacc gaggtggaca
     3241 gcatctggaa ggatccgacc gcggtgggcc cgaagggcaa cctgctcatc gtgggattga
     3301 gcgacaacca gacgagccgg cgcatgttcg aggacgcgtt gaccagcacg ctgaccgcgg
     3361 ccggcaacca ggccagccag agctacccgg tcatcccgag cgggcgcgag gccgacaagc
     3421 aggcgatgac gggcatgctc cgcgagaagg gtttcgacgc catcctgacc gcgcgcgccg
     3481 tgtccgaaga caaggaaaag atctacaacc ccgagccgga gtacttcacg ccgccgagct
     3541 actactacag ctactgggac ttctactcgc cggccacgca ggccgtgtcc gcgccgggct
     3601 actacaccga ggaaacatgg gtccgcgtcg agaccaacct ctacgacgcc gacggcaagc
     3661 tgatctggac cgcggccacc tccaccctca agaacgggga catgagggag gagatccaca
     3721 gcctctgcgc gacgctgacg aagcagctgg agaagtcggg cctcatcaag tagaaggccc
     3781 ggcgcccctc gacaagtcgt cactgtcagg gtgacactcc cgccatgccg ctgaccgcag
     3841 gatctcgcct ggggacctat gaaattctcg ggcccctcgg cgccggggga atgggggagg
     3901 tgttccgcgc ccgcgattcc cgactggggc gcgacgtggc catcaaggtc ctgcctgacg
     3961 ccttcgccca ggacccggag cgactggccc gcttcgaacg ggaagcgcgc ctgctcgcgt
     4021 cgctcacgca tgccaacatc gccggaatcc acggcctcga agtggtggat gggcatcggc
     4081 atctcatcct cgagttcgtc gatggggaaa cgctggccaa ccggctggcc cgcggcccgc
     4141 tcccactcga cgaggcgctc ggcatcgcct cgcagatcgc cggggccctc gatgccgccc
     4201 acgaagccgg aatcgtccat cgcgatctga agcccggcaa cgtgatgctg accgccgccg
     4261 gcgacgtgaa ggtcctcgac ttcggcctgg cccgcggcgg gccggcgagc tcgagcgatc
     4321 cgtcgctctc cgcctcaccc acgatgacct acgccgccac gatggccggc gtcatcctcg
     4381 gcacggcggc gtacatgagc ccggagcagg cccgcggcaa ggcggtcgat cgtcgcaccg
     4441 atatctggtc gttcggctgc gttctctacg agtgcctgac ggcccgggtc ctcttcgccg
     4501 gggagacggt ttcggacacg atcgcgaaga tcctggaacg cgagccggac tggagcgccc
     4561 tgccggagcg cacgccgccc cgcgtgcgcg agctcctgcg gcgctgcctc gagaaggatc
     4621 cgaagaaacg ccagcgcgat gccggcgata tccggctgga gctggaggag atcctggcgg
     4681 gcggaatgtc gtcgagtgcg gcgtcgggtt cggccggcgc cgccccgccc cggggacgcc
     4741 ccgcccgact cccctgggtc atcgccggcg tctcgctcgt cttggctctc gtttcgctcg
     4801 ccgggttgtt ccgcccgccg ccgccgtcac cgccgccgct gcgcacctcc atcacctttc
     4861 cgcccggcgt cgggctgttc gaatgggagg cgtccgacct ggccgtgtcg ccggacggaa
     4921 gcctgctggc ctgggcgggc aatgattcga cggagaagag cggcgtgtgg gtgcgtccgc
     4981 tcgacgggct ggaagcacgg ctggtggccc ggctcgatgc ggggccgggg ctacccttct
     5041 ggtcacccga ctcgcggcgc gttgcctatt tcgccgacgg caagctccgg gtggtgggac
     5101 tggacggcaa tccgccgcag gtcatctgcg acgcgctcga tggtcgcggc ggcacctgga
     5161 acgcggacgg cgtgatcctc ttcacgccca ccagcgcggg gcccatccac cgcgtggcgg
     5221 cggccggcgg caagtcggag ccgatcctcg agctggatgc ggcggccggc gaaaccgggc
     5281 agcgctatcc gcgcttcctc cccgacgggc gccacttcac gttcacctcg gtgcccgccc
     5341 gcgatggcgg cctcctctcg tatgtgggcc ggctcggttc gaacgagcgc cacttcctgg
     5401 cctcggccac caccggcgcc atcctggccg cgccgggcca ggtggtcttc gcccgcgaca
     5461 acaacctcat ggtgcagcga tgcaacccca cgaactggaa gctgatcggc gacccggaaa
     5521 cgatcgcatt cgggctgggg aggtaccgca ccatcggtcg tcccgccgcg gcggccacgg
     5581 ccagtctcct catctttccc agcttcaaga cacgcgacta ccgactcgcc tggatcggcc
     5641 gcgacgacgg catccggcaa ccgctcgagg tgccgagcgg tttctggagc ggtgtctatc
     5701 ccagtcccga cggccggaag gccgtggcct tccggctaag cgacgacggc aagctccgcc
     5761 tgtggatgat cgacaccgtc cgcgggaccg gcgcccagat gcccgaggga gacgacacgt
     5821 tcggtccggc cgcctggacc cccgacggtc gcgagttcta ctacggcaag cgcatcggca
     5881 cccggcagga cttctacgtc gggagcgcgg cgggctccga gccggcacgc ccattcctcg
     5941 aggggggaat gctgttcaag gggagtccgg acgtgtcgcc cgacggacgc tacctcctgt
     6001 tcgcccagct cgaggccacg acgcggcgcg acatctggta cgcaccgatc gccggcggcg
     6061 atcccgtgcg cctgatcggc ggcccggcgg aggaagccgg cccggccttc tccccggacg
     6121 gccgctgggt cgtgtaccag gcccaggagt ccggccgacc cgagttgtgg gtggcgccgt
     6181 tcccgtcggg aaagggcgcc atgcagatca ccacggccgg cggtaccgga ccgacctggg
     6241 tggccggcac ctgggagatc ctgtacgtgg acatgtcggg acacgtcacc agcatcccgg
     6301 tcaccccggg cccctctccc gtattcggca cgcccacacc catccgtgcc ctcgcaggcc
     6361 tcgaggatcg cctccagacg gccaaggcca cgcgcgacgg ccgccgcttc ctcgtctcga
     6421 tcaccgacca ggaatcgcag gaccccacgc tgacggtgat ttcgaactgg caggggctgc
     6481 tggaaaagcc gtagggcctg tatttcacgg cttgatctcg ccggactttc tccttgaacc
     6541 gacaacctgc cgctggtagt ctcaatccgt accgatttgg tccacttttg gtcatcccgc
     6601 cgcatcattg agggaggccc cccgagttgc ttcgcatcac caagcaggcc gactacgcca
     6661 tcgtgctcct cagtcat
//
LOCUS       DASVOD010000317        13840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_595021, whole genome shotgun sequence.
ACCESSION   DASVOD010000317 DASVOD010000000
VERSION     DASVOD010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13840
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595021"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1384)
                     /locus_tag="VF720_07120"
     CDS             complement(<1..1384)
                     /locus_tag="VF720_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7879164.1"
                     /translation="MRRIRCLQSSLPSVLVMAALVASTIPALAAESTPGLRGHLSSES
                     GAALDEVEVIILELKREARTDENGNFQFGEIPQGTYTVMIQGLGFAPVTRTLALPTGE
                     PLEVTLESRVLEMAGVEVTGSRVPRPALDSPLPTARLSHHELERETGISVAHAVEELP
                     GVRVVSTGQQIGKPMIRGLTGARVLMLDDGHRIEDYSWSDEDGPSVDPTSAERIEVVR
                     GPASLMYGSDAIGGVINTLPPEVPETEEGEAAFFRGKPSFYYGTNNSEFGLGLNLEGA
                     RADWGWRFNVIGRKAESFQTPDEEVEHTGFESVNGDAALGVHGRKGNASIRYARYGGE
                     FKLLEGGGPPPGQEEGEEEGPERKSSDDRVQFNGNLLAGSTRFEAKAQWQAHSLIELS
                     DEFLPEGEGAPSLHADRHESTILAPATAAEKREMEAFNLLLHTTTLDLMAHHRLGSNI
                     NGTAGVTGFLQ"
     gene            complement(1540..3270)
                     /gene="polX"
                     /locus_tag="VF720_07125"
     CDS             complement(1540..3270)
                     /gene="polX"
                     /locus_tag="VF720_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012996084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX7879165.1"
                     /translation="MDKRAVADILERIGTLLELKGENVFKTRAYQNGARALMALEEDL
                     AVLVAENRLKDVKGIGAALCEKITLLVTTGRLEYYEKLKAEFPDGFEELLRVPGFGPK
                     KAMLVMTELKIATLDELEAAARAGSLAALPGFGAKTEQKILEGIEHVRKHAGRFLANV
                     AESEANLLLAAIRDLPGVERVSLAGSLRRRLETSKDIDIVVAAREAAPIMAAFTSAPN
                     VAAVTGHGETKSSVRLASGMAADLRCVTPEEFPFALMYFTGSKAHNVAMRGRAQKMGW
                     RLNEYALEKNDKPGVMKPCADEAAIYEALGLRYIEPELREDTGELAASEAGTLPRLIT
                     DADIRGLLHQHTDWSDGQDTLDEMVRTAAGIGLEFFGITDHSQTAGYARGLSPERVRK
                     QHHAIEALQAVYAGKLRIFKGIESDILPDGRLDYDEETLALFDYLVASVHSSFQLSEA
                     DQTARIVRAVSHPKVRILGHPTGRLLLARDPYAVNLSHVFAACAANEVAVEINASPHR
                     LDLDWREIRAAKEAGCRFSINPDAHNTHGLAEERFGVGVARKGWLTAADVINTWDADA
                     IERFLRRGQV"
     gene            3298..4059
                     /locus_tag="VF720_07130"
     CDS             3298..4059
                     /locus_tag="VF720_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015810027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEX7879166.1"
                     /translation="MLTDLTDPQAWIALLTLTVMEIVLGIDNVIFISILAGKLPTAQR
                     DRARTIGLAAAMITRILLLLGLTWVMKLTEPLFNVLHHDVSGRDLILIIGGLFLIAKS
                     THEIHEKLEGEEHHDAARAAAGFAGVIVQIMLLDIIFSLDSVITAVGMAQHLWVMVTA
                     VVIAVIFMMVYSGPVSRFVDRHPTVKMLALSFLILIGVTLVAEGFHQHIPKGYIYFAM
                     AFSVLVEMLNLRLRKSGTPVKLHHTPHDRPAEPRS"
     gene            4073..4765
                     /locus_tag="VF720_07135"
     CDS             4073..4765
                     /locus_tag="VF720_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX7879167.1"
                     /translation="MNAHDLPERLPVFPLPEIVLFPGVPLPLHIFEPRYRAMMGDVLS
                     RPADQRLIVMALLRPGWEADYDGTPPTYPIATVGEILHSKSLDDGRYLITLRGLERIR
                     LTGEEAVTDGGYRVARVARAPESADGLSGSGVVDALVRLLDDFAAWTGGVPISVRRED
                     LEARQEARGLVLNTLAFHLSSAAGTRQELLEEDDLARRLERLARIVADGLGGKRALDA
                     WRPKRPDDPGVN"
     gene            4767..5540
                     /gene="nth"
                     /locus_tag="VF720_07140"
     CDS             4767..5540
                     /gene="nth"
                     /locus_tag="VF720_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX7879168.1"
                     /translation="MAAARKKATSRPSSGRKPSAKPSEPALDAARYQPKRRRDLPESA
                     RAPARERISPILAALDREYAVAECALDHEKPLELLAATILSAQCTDERVNIVTKDLFP
                     RYPTPKHYAEADPDELESVIRSTGFYRNKTKSLIGMGKGLVERFGGLVPRTMDELLSL
                     PGVARKTANVVLGTAFGIPEGVVVDTHVGRIAQRLGLTKNVDPVKIEQDLMRLVPREH
                     WIRLSHQLIWHGRRICTARKPDCPNCPLRDWCPSADRAY"
     gene            complement(5559..6941)
                     /locus_tag="VF720_07145"
     CDS             complement(5559..6941)
                     /locus_tag="VF720_07145"
                     /inference="COORDINATES: protein motif:HMM:NF016146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEX7879169.1"
                     /translation="MRALVAIVAFIVLATGDGARAAYPPKGTVLFPGLVGTALQDSIR
                     IRFRPPVGLNYAQARDQMYTTIDNHDGDVVCVYTGFTIHVDPQSSQPRTDAFNAGINA
                     EHTWPQSKGAEVPPMEADLHHLFPSEIDANNRRGNLPFDEIPDTETDTWWINHTNTGT
                     APALGVRDNYSEYDGTFAGTPYPGRWEPREVHEGNVARAMFYFWTVYRAEANAADPSF
                     FNVQKDALRQWAMVDTADLAEYLRTCAVADLQSGKYNPFVVDPTLIDRAYFNDVPVEL
                     LAFTASPNGAAVALAWETRSETGHAGFNVLRVSPGKGSEARRTTRMNERLLTQGPRYT
                     LTDDTGTPGLTYDYYLEAVARDGSVERFGPRSIRFPDEAPGAGRWQLGPNPFRAGGRL
                     TLSGARSSLTSVEIFDSTGRRLPLADGAVLLDGWDGRLDNGRSIRPGIYFVRIRAGND
                     VAIFRQAIVR"
     gene            complement(6950..7819)
                     /locus_tag="VF720_07150"
     CDS             complement(6950..7819)
                     /locus_tag="VF720_07150"
                     /inference="COORDINATES: protein motif:HMM:NF027524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879170.1"
                     /translation="MKKPRRYHLFSDYLRNRFGDRLQKVTLSGGLVCSHPEGPAACDY
                     CRAAREADDSWGRGAPPHEQIRRGINFSRRNHHPEPHLHVTIPNHQTFAPDIDHLEAT
                     LVTVSAIPEVSVVSFWTRAEWANDSVANLLARFADDALEVWLELDDAPAAWPAGRPEE
                     LRYGVQVDLGLGPAPAPANGHSAESAARVVQQLKPDAVGIKAPAVVAGTSEAELYERG
                     RLDDVELDAFAERAAEFLERIPGDVAVHPLVLNAPGDSIVAPHWVHNRQKVEEAVSLA
                     LEARGAVQGGKAE"
     gene            complement(7816..8697)
                     /locus_tag="VF720_07155"
     CDS             complement(7816..8697)
                     /locus_tag="VF720_07155"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7879171.1"
                     /translation="MGDSPNPTPAEPPAPPFDPPANLHIGTSSWSSDDWRGTVYEAGA
                     KPGDYITQYARSYRTVEIDATFYRIPSDTMVDGWREKTPDNFVFSAKIPQVITHEKEL
                     VDCEDDFRLFLSRMERLREKLGPMVFQFPYVAKGKDADEYATGAGFRERLERFLKCLP
                     SDHRFAVEVRNEKWIDEAFLDLLRRHKVALVLVEYFTMPAMTKLVSKLDCQTGDFTYL
                     RFLGDHKRMDALVKERDKPWGSLAVDRTNETRRWIAPVNGFLAKKIDVFAYFNNHFAG
                     YAPGSVKLFLDLLRETS"
     gene            8920..10188
                     /locus_tag="VF720_07160"
     CDS             8920..10188
                     /locus_tag="VF720_07160"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX7879172.1"
                     /translation="MTVNMTKTVFDHAMEQFDAAARLMKLNQNQIAMVKEPRRATEVN
                     LPVQMDNGTIELFKAFRVQHSIIRGPAKGGIRYHPNVSLDEVKALAFWMTYKCAVVDV
                     PFGGGKGGIIVDPFRLSGAELERLSRRYFAELCDLFGPDMDVPAPDVNTNPTIMGWMM
                     DTYSMHEKKYLPAVITGKPLEVGGSKGRTQATALGVIYTIRQAAQHLKIDLSKSRAAV
                     QGFGNVGSYSAQFLYEDGATVVGIADVTGSYFNPKGINIPEAMKYSQENRGLRGYDKL
                     SGCTYHENTNAVLTAECDVLVPAALEEVISPEIAAKISAKIVAEGANGPTTGPADIVL
                     AERGVFMIPDILCNSGGVTVSYLEWVQNRMGYYWEEERVISDLKRFMTKAFDDVLAMS
                     LEHKVNMRIAAFMLGIQRVTRAAEIRGLYA"
     gene            10205..10999
                     /locus_tag="VF720_07165"
     CDS             10205..10999
                     /locus_tag="VF720_07165"
                     /inference="COORDINATES: protein motif:HMM:NF028494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879173.1"
                     /translation="MHLRPVAIGLAVAAGLVFSGCSKKSDTAGGGAPRAKRAPGSPPP
                     FALPPNNQPATYIGTFDDTPSWSERWTVWLRPDSTYFLREEHVGHGDVASDHSEDDIG
                     RWERATDGGPIRLQGGRNQPFELDVPHPDTLRFRIVDQALGVEPHFLVRTDSIAYFEP
                     RVSLTGTLRLTADAALFDDCASSLRWPVLKEEGFSAAEVAYRQSAVNPGDALLVSIEG
                     RVVRRPRMNDGPARDYLVIERFGQWTPGGRCGEPSPAEGLPYGAGK"
     gene            10996..11967
                     /locus_tag="VF720_07170"
     CDS             10996..11967
                     /locus_tag="VF720_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879174.1"
                     /translation="MTTSNHSRRIAAIAIASIAAGHTLYACSKPVDRSEHVPTVQARP
                     VASASRSIFPPDRLPAAYFGKVDLPAGRSERWTIWLKPELYFLRVEYREKAGFSSEDI
                     IGRWDTIADRTIRLHADGRDVIELEVPDLDTLRLGAAVGQHGTRPPALLARTSSIHYL
                     EPHISLTGYMSCSDDGGYFQDCGTGWGLPILKEGDFETALREYHNVRPDRRSPEQDLI
                     FDRLPRPSQSTSRVSPHSLVESESAGKVMPRPMPVPRPMPVPPRVFVSLDARIVLRLS
                     VNGASAGEALVIERFGRVIRGKACADTSALAERERQERLASLPYGIR"
     gene            11964..13028
                     /gene="queG"
                     /locus_tag="VF720_07175"
     CDS             11964..13028
                     /gene="queG"
                     /locus_tag="VF720_07175"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX7879175.1"
                     /translation="MTNPAEYSRLIREAALRHGFHHVGIARAREVRHADEFRAWLADG
                     RHASMSYMEKRVDERLDPELLDPAYQTIVSVASSYFHDNAAPAATEPASSLPAGRIAR
                     YAHGADYHNVLGKQLRKLLKELRMSLDGCDGRAWVDTGPVMDRAWASRAGIGWWGKHT
                     TLVSRTDGSWLVLGTMALTIECEPDEPHEDFCGSCHRCIDACPTKAIVAERELDARLC
                     ISYWTIEHRGTISEAMRPAIGDWIFGCDLCLEVCPWNRFAQAGREARFAAREDRAAPA
                     LEPLLDLDDAAFRIKFNGSPIQRATRDGFVRNVCVALGNVGTPRNVRALERIAGNDAS
                     EVVREHAHWGIQRIQERSGE"
     gene            13029..>13840
                     /locus_tag="VF720_07180"
     CDS             13029..>13840
                     /locus_tag="VF720_07180"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HEX7879176.1"
                     /translation="MSRTILVTGATGAQGGSVAHALLTHGGFNVRALTRHPDSDKARA
                     LKAAGAVPVAGDLADRASLEAALAGCSACFGVTNFWEHFEAEFDHGKNLIEAVAAAGI
                     RDFLYSSLPPIEKSTDGKLKAPHFDLKAELETLARRLVPGSAFVHLMFYYENFLAGSA
                     PQRTPDGSYAFGFPQGDVPLAGVSAEDVGGAIAPIFADFDAWRGKAIPIVGDILPPAE
                     YAAILSRVTGARVLYSHIPLEVFSKFPFPGADDLANMFEWYRLYMPDRHAAW"
BASE COUNT         2288 a         4678 c         4463 g         2411 t
ORIGIN      
        1 actgcaggaa gccggtcacg ccggcggtgc cgttgatgtt gcttccgagc cggtggtgcg
       61 ccatcaggtc gagcgtcgtg gtgtgcagga ggaggttgaa tgcctccatc tcgcgttttt
      121 cggccgcggt ggccggggcg aggatcgtcg attcgtgccg atcggcatgc agtgagggcg
      181 ctccctcacc ctcgggcagg aactcgtccg acagctcgat cagcgagtgc gcctgccact
      241 gggccttggc ctcaaaacgc gtcgagccgg ccaggaggtt gccgttgaac tgaacgcggt
      301 cgtccgacga cttgcgctcg ggtccttcct cttcaccctc ttcctggccg ggagggggtc
      361 cgccaccttc gaggagcttg aactcgccgc catagcgggc gtaacggatc gaggcgttcc
      421 cctttctgcc gtggacgccg agcgccgcgt cgccgttcac cgactcgaac ccggtgtgct
      481 ccacttcctc atccggcgtc tggaacgact cggctttacg cccgatcacg ttgaagcgcc
      541 atccccagtc ggcgcgcgcg ccttccaggt tgaggcccag gccgaactcg gagttgttcg
      601 tgccgtagta gaaggagggc ttgccgcgga agaacgccgc ctcaccttcc tcggtctcgg
      661 gcacctcggg cggcagagtg ttgatcacgc cgccgatggc gtcggagcca tacatcaggc
      721 tggccggtcc ccgcaccacc tcgatccgct cggcgctggt cggatcgacc gaaggaccgt
      781 cttcatcgct ccacgaatag tcctcgatac ggtgcccgtc atcgagcatc agcacgcgcg
      841 cccccgtcag gccgcggatc atcggcttgc cgatctgctg gccggtggag acgacgcgca
      901 cgcccggcag ctcctcgacg gcatgcgcca ccgagatacc cgtctcacgt tcgagctcat
      961 gatgggagag gcgcgccgta gggagggggg aatcgagcgc ggggcgcggc acgcgtgagc
     1021 cggtgacttc aacacccgcc atttccagca ctcgcgattc gagagtcacc tcgagtggct
     1081 cgccggtcgg cagcgccagc gtccgcgtga cgggcgcgaa gccgagcccc tggatcatca
     1141 cggtataggt accctgcggg atttcgccga actggaagtt gccattctca tccgtgcggg
     1201 cttcgcgctt cagctcaagg atgatgacct cgacctcgtc gagcgccgcg ccggactccg
     1261 agctgagatg accgcgcagg cccggcgtgc tttcggccgc gagtgccggg atggtgctgg
     1321 cgacaagtgc cgccattacc agaacagaag ggagggaaga ctgcaggcag cgtatccgtc
     1381 gcatgcgcgg gtggcctcct cgagccacgt ctttcataga gcaccagccg gcacggcacg
     1441 gatcgaggca gatgcggcgc accggatcgg ggcgcattct agggacatga atatcagaat
     1501 tgggtcagaa tgaagtccat gtgcttcagt gacagacagt tacacctggc ctcgtcgcag
     1561 aaaccgctcg atcgcgtccg catcccacgt gttgatcacg tccgccgccg tcagccaccc
     1621 cttccgagcc acgcccacgc cgaagcgttc ctctgccagt ccgtgggtgt tgtgcgcatc
     1681 cgggttgatc gagaagcggc aacccgcctc cttcgccgcg cggatctcgc gccagtccag
     1741 atcgaggcga tgcgggctgg cgttgatctc caccgccacc tcgttggccg cgcaggcggc
     1801 gaagacgtgg gagaggttca ctgcgtacgg atcgcgcgca aggagaagcc tcccggtggg
     1861 atggccgagg atccgcacct tcggatgact cacggcccgc acgatccggg ccgtctgatc
     1921 ggcctccgaa agctggaagc tcgagtggac cgaggccacc aggtaatcga agagggccag
     1981 cgtctcctcg tcgtagtcga gccgcccgtc cggcaggatg tcgctctcga tccccttgaa
     2041 gatgcggagt tttcccgcgt acacggcctg cagggcttcg atggcgtggt gttgcttccg
     2101 cacccgttcg ggggaaaggc cccgcgcgta gccggccgtc tggctgtgat cggtgatgcc
     2161 gaagaactcg aggccgatgc cggcggcagt gcgcaccatc tcgtcgagcg tgtcctggcc
     2221 gtcgctccag tcggtgtgct ggtggaggag gccgcgaatg tcggcgtccg tgatgaggcg
     2281 cgggagcgtg ccggcctcgg aggcggccag ttcgcccgtg tcctcgcgca actcgggctc
     2341 gatgtagcgc agcccgagcg cctcgtagat ggccgcctcg tcggcgcagg gcttcatcac
     2401 cccaggcttg tcgtttttct cgagcgcgta ctcgttcagc cgccatccca tcttctgggc
     2461 ccggccgcgc atggccacgt tgtgcgcctt gctccccgtg aagtacatga gagcgaacgg
     2521 gaattcctcg ggagtcacgc agcgcaggtc ggccgccatt cccgatgcca gccgcacgct
     2581 cgatttcgtc tcgccgtgac ccgtgacggc cgccacgttg ggcgccgagg tgaacgccgc
     2641 catgatcggc gcggcctccc gtgcggccac cacgatatcg atgtccttgc tggtttccag
     2701 gcgtcgccgc agacttcctg ccaggctcac gcgctccacg ccgggcagat cgcggatggc
     2761 ggccaggagc aggttggcct cgctctcggc aacgttggcc aggaagcgtc ccgcgtgctt
     2821 gcggacatgc tcgatcccct ccaggatctt ctgctcggtc tttgcgccga agccggggag
     2881 ggcggccagg ctgccggcgc gggccgctgc ctccagctcg tcgagcgtgg cgatcttgag
     2941 ttcggtcatc accagcatgg ccttcttcgg tccgaagccc ggcacgcgga gcagctcctc
     3001 gaagccgtcg ggaaattcgg ccttcagctt ctcgtagtac tcgaggcgcc cggtcgttac
     3061 cagcagggtg atcttctcac agagcgccgc cccgatcccc ttcacgtcct tgagccggtt
     3121 ctcggccacc agcacggcca ggtcttcctc cagggccatc agggcccggg ccccgttctg
     3181 gtaagcccgg gtcttgaaga cgttttcccc cttcaactcg aggagcgtcc cgattcgctc
     3241 caggatgtcg gctacggctc gcttgtccac agtcaccctc tcgaatagag ttccgcgatg
     3301 cttaccgacc tgaccgaccc gcaggcctgg attgccctcc tgacgcttac cgtcatggag
     3361 atcgtcctcg gcatcgacaa cgtcatcttc atttcgatcc tggccggcaa gctcccgacc
     3421 gcgcagcgtg atcgcgcccg caccatcggc ctggccgccg ccatgatcac gcgcatcctg
     3481 cttctcctcg gcctcacctg ggtgatgaag ctcacggagc ccctcttcaa cgtcctgcac
     3541 cacgatgtat cgggccgcga cctgatcctg atcatcggcg gcctcttcct catcgccaaa
     3601 agcacgcacg aaatccacga aaagctggag ggggaggagc atcacgatgc cgcccgcgcc
     3661 gccgcgggct tcgcgggggt catcgtgcag atcatgctac tcgatatcat cttctcgctc
     3721 gactcggtca tcaccgccgt cggcatggcc cagcacctct gggtcatggt gaccgccgtc
     3781 gtcatcgcgg tgatcttcat gatggtctac tcggggccgg tgtcgcgctt tgtcgaccgg
     3841 caccccacgg tcaagatgct ggcgctctcg ttcctcatcc tgatcggcgt cacgctcgtg
     3901 gccgaagggt tccaccagca cattccgaag ggatacatct acttcgcgat ggccttctcc
     3961 gtcctcgtgg agatgctgaa cctccgcctt cggaaatcgg gcacgccggt caagctccac
     4021 cacacccccc acgatcgacc tgccgaaccg cggtcctgag gaccgcaccg acatgaacgc
     4081 gcacgatctt ccggagcggc tgccggtctt tccgcttccg gagatcgtgc tcttcccggg
     4141 cgttccactg ccgctgcaca tcttcgaacc gcgttatcgg gccatgatgg gagatgtgct
     4201 gtcacgcccc gcggaccagc gcctcatcgt catggcgctg ctccggccgg gatgggaagc
     4261 ggactatgac gggacgccgc cgacatatcc gatcgccacg gtgggggaaa ttcttcactc
     4321 gaagtcattg gatgacggcc gctatctcat cacgctccgc ggtctcgagc gtatccgcct
     4381 gaccggcgag gaagcagtga ccgacggcgg ctaccgggtg gcacgcgtgg cgcgggcgcc
     4441 cgagtccgcg gacggcctct ccgggtcggg tgtcgtcgac gcgctggtgc gcctcctcga
     4501 cgacttcgcc gcctggaccg gcggcgtgcc gatctccgtt cgccgggagg atctcgaagc
     4561 ccgccaggag gcccgcggcc tcgtcctcaa caccctggcc ttccacctgt cctccgcggc
     4621 cgggacaaga caggaactcc tggaggaaga cgacctggca cgccgcctcg aacgcctcgc
     4681 ccgcatcgtg gcggacggcc ttggaggaaa gcgggccctc gatgcgtggc gcccgaaacg
     4741 gcccgacgac cccggggtga actgacatgg ccgctgcgcg caagaaggct acgagccgcc
     4801 catcatcggg gaggaagcca tcggcgaagc cgtccgaacc ggcactcgat gccgcccgct
     4861 atcagccgaa gcgccgccgt gacctccccg agtccgcccg cgccccggca agggagcgaa
     4921 tctcgccgat cctggccgcg ctcgatcgcg aatacgccgt ggccgaatgt gccctcgatc
     4981 acgagaagcc cctcgaactc ctggccgcca cgatcctgtc ggcgcagtgc accgacgaac
     5041 gcgtcaacat cgtcacgaag gatctcttcc cgcgctatcc gacgccgaaa cactacgcgg
     5101 aagccgatcc cgacgaactc gaatccgtca tccgctccac cggcttctat cgcaacaaga
     5161 cgaagagcct gatcggaatg ggaaaaggtc tggtggaacg cttcggcggc ctggtgccgc
     5221 gtacgatgga cgagctcctg tcgcttcccg gcgtggcgcg caagaccgcc aatgtcgtcc
     5281 tgggcacggc attcggcatc ccggaaggcg tggtcgtcga tacgcatgtc gggcgcatcg
     5341 cccagcgtct cggcctcacg aagaatgtcg acccggtcaa gatcgagcag gacctgatgc
     5401 gcctcgtgcc gcgcgagcac tggattcggc tcagccacca gctcatctgg cacggccgca
     5461 ggatatgcac cgcccgcaag ccggactgcc cgaactgccc gctgcgggat tggtgtccgt
     5521 cggcggaccg cgcctactga ggcgcgcgcc gggccaaact agcgaacaat ggcctgccgg
     5581 aagatcgcca cgtcgtttcc cgcgcggatc cgcacgaaat aaatcccggg gcggatcgac
     5641 cgcccgttgt cgagacggcc gtcccatccg tcgagcagga ccgctccatc cgcgagcggc
     5701 agccgacgcc cggtggaatc gaagatttcg accgaggtca acgaagaacg agccccgctc
     5761 agcgtgagcc gaccgcccgc ccggaacgga ttggggccga gttgccagcg ccccgcaccc
     5821 ggcgcctcgt cgggaaagcg gatcgaccga gggccgaatc gctccaccga gccgtcgcgg
     5881 gccacggcct cgaggtaata gtcgtaggtc aggcccggcg ttccggtgtc atcggtcagc
     5941 gtgtagcgcg gcccctgcgt gagaaggcgc tcgttcatcc gcgtggtgcg ccgcgcttcg
     6001 ctccctttcc cgggcgacac gcgcaacacg ttgaaaccgg cgtgaccggt ttccgagcgc
     6061 gtttcccacg ccagcgccac ggcggcgcca ttcggcgagg ccgtgaaggc caacagctcg
     6121 accggcacgt cgttgaaata cgcccggtcg atcagcgtcg gatcgacgac gaaggggttg
     6181 tacttgccac tctgcaggtc ggccaccgcg caggtacgga ggtattcggc caggtcggcg
     6241 gtgtcgacca tcgcccactg ccgaagggcg tctttctgca cgttgaagaa gctgggatcg
     6301 gccgcgttgg cttcggcgcg gtacacggtc cagaagtaga acatcgcgcg ggcgacgttt
     6361 ccttcgtgga cttcgcgggg ctcccaccgg cccgggtacg gtgttccggc gaatgtgccg
     6421 tcgtactcgg aatagttgtc gcgcacgccg agggccggcg ctgtgccggt attggtgtgg
     6481 ttgatccacc acgtatcggt ctcggtgtcc gggatctcat cgaagggaag attgccgcgt
     6541 cgattgttcg cgtcgatctc gctggggaac aggtggtgga ggtcggcctc catcggtggt
     6601 acttcggccc ccttgctctg cggccaggtg tgctcggcgt tgatccccgc gttgaatgcg
     6661 tcagtgcggg gctggctcga ttgcggatcg acatggatcg tgaagccggt atacacgcag
     6721 acgacgtcac catcatggtt gtcgatggtc gtgtacatct ggtcccgcgc ctgcgcgtaa
     6781 ttgagcccaa ccggcggccg aaaacggatc cggatgctgt cctgcaacgc ggtacccacc
     6841 aggccgggga agaggacggt gcctttcggc gggtacgcag cgcgggcccc atcgcccgtc
     6901 gccaacacga tgaaggcaac gatggcgacg agcgcccgca ccgacggcct cactccgcct
     6961 tccccccctg caccgccccg cgcgcctcga gcgccaggct cacggcttcc tccaccttct
     7021 gccggttgtg aacccagtga ggcgccacga tcgagtcgcc cggggcgttg agcacgaggg
     7081 ggtggacggc cacgtcgccg gggatccgtt cgaggaattc ggcggcgcgc tcggcaaagg
     7141 cgtcgagctc cacgtcgtcg agccggccgc gctcgtacag ttccgcctcg ctggtgccgg
     7201 ccaccaccgc cggggccttg attcccacgg cgtccggctt cagttgctgc accacccgtg
     7261 cggcggactc ggccgaatgg ccgttggcgg gcgccggcgc cgggccgagg cccaggtcca
     7321 cctgcacgcc gtaacggagc tcctcgggcc ggccggccgg ccaggcggcg ggcgcgtcat
     7381 cgagctcgag ccacacttcc agcgcgtcgt cggcgaatcg cgccagcaga ttggcgacgg
     7441 aatcgttcgc ccactcggcg cgcgtccaga acgacacgac cgacacttcg ggaatggccg
     7501 acacggtgac cagcgtggct tccaggtggt cgatgtccgg cgcgaatgtc tggtgattgg
     7561 gaatcgtcac gtgcagatgc ggctcggggt gatggttgcg ccgcgaaaaa ttgatgccac
     7621 gccggatctg ctcgtgcggc ggggcgccgc ggccccagga gtcgtcggcc tcgcgggcgg
     7681 ccctgcagta atcacaggct gccggtcctt cgggatgcga gcagacgagt ccgcccgaca
     7741 gcgtcacctt ctggaggcgg tcgccgaagc ggttgcggag atagtcggaa aacaggtggt
     7801 agcgccgcgg ttttttcatg acgtctccct caggagatcg aggaaaagtt tgacacttcc
     7861 cggcgcgtag ccggcaaagt gattgttgaa ataggcgaac acgtcgatct tcttcgcgag
     7921 gaaaccgttc accggcgcga tccaccggcg ggtttcattc gttcgatcca ccgcgaggct
     7981 gccccacggc ttgtcgcgct ccttgaccag ggcatccatc cgcttgtgat cgccgaggaa
     8041 gcggaggtag gtgaaatcgc cggtctggca gtcgagcttg gagaccagct tcgtcatggc
     8101 cggcatcgtg aagtattcaa cgaggaccag cgccaccttg tgtcgtcgga gcaggtcgag
     8161 gaacgcctcg tcgatccact tctcgttgcg cacttccacg gcaaagcggt ggtcggacgg
     8221 caggcacttc aggaatcgct cgaggcgctc ccgaaagccc gcaccggtcg cgtattcatc
     8281 ggcgtccttt cccttagcca cgtagggaaa ctggaacacc atcggcccca gtttttcgcg
     8341 gagccgttcc atgcgggaca ggaacaggcg gaaatcgtct tcgcaatcga cgagctcctt
     8401 ctcgtgcgtg atgacctggg gaatcttggc gctgaagacg aaattgtccg gggtcttctc
     8461 gcgccagccg tccaccatcg tatcggaggg gatgcggtag aaggtggcgt cgatctcgac
     8521 ggtgcggtac gagcgcgcgt actgggtgat gtaatcgccc ggtttggcgc cggcctcgta
     8581 caccgtgccc cgccagtcgt cgctcgacca cgagcttgtt ccgatgtgca ggttggccgg
     8641 cgggtcgaac gggggagcgg gcggctcggc aggtgtcgga ttcgggctgt cccccacgtt
     8701 cccatccttt ccgggcgagc gttggagccc acgcccaggg cggttcgaat cgccgtcccg
     8761 gccagcatac gcccggcagg caggccgctc caacacgtcc attccggacc gggttccgcc
     8821 ctcctggccg tctggcggcc cgccggtcca atctggtaaa ttttcgccct caacgccaca
     8881 cgccgaccct cacctccggc acccaacgag gaccctgcca tgaccgtcaa catgacgaag
     8941 accgtattcg accacgccat ggagcaattc gacgcggcgg cccggttgat gaagctcaac
     9001 cagaaccaga tcgcgatggt gaaggagccc cgccgggcca ccgaggtgaa cctgccggtc
     9061 cagatggaca acggcacgat cgaactcttc aaggccttcc gcgtccagca ctcgatcatc
     9121 cggggcccgg ccaagggcgg catccgctac cacccgaatg tctcgctgga tgaggtgaag
     9181 gcgctggcgt tctggatgac gtacaagtgc gcggtggtcg atgtgccctt cggcggcggc
     9241 aagggcggca tcatcgtcga tccgttccgc ctctcgggcg ccgagctcga gcgcctctcg
     9301 cgccgctact ttgccgaact gtgcgacctg ttcggccccg acatggacgt gcccgccccc
     9361 gacgtgaaca ccaatcccac gatcatgggg tggatgatgg acacctactc catgcacgag
     9421 aagaagtacc tgccggccgt catcaccggc aagccgctcg aagtgggcgg cagcaagggg
     9481 cgcacgcagg ccaccgccct cggcgtcatc tacacgatcc ggcaggcagc ccagcacctg
     9541 aagatcgacc tgtcgaagag ccgcgccgcg gtgcagggct tcggcaacgt cggttcctat
     9601 tcggcccagt tcctgtacga ggatggcgcc accgtcgtcg gcatcgccga cgtcaccggc
     9661 agctacttca accccaaggg gatcaacatc cccgaggcca tgaagtactc gcaggaaaac
     9721 cgcggcctcc gcggctacga caagctctcc ggctgcacat atcatgagaa caccaacgcc
     9781 gtcctcaccg ccgagtgcga tgtgctcgtg cccgccgcgc tggaggaggt catctccccc
     9841 gaaatcgccg cgaagatctc ggccaagatc gtggccgaag gcgccaacgg ccccaccacc
     9901 ggcccggccg acatcgtgct cgccgagcgc ggcgtcttca tgatccccga catcctctgc
     9961 aactcgggcg gcgtcaccgt cagctacctc gagtgggtgc agaaccgcat gggctactac
    10021 tgggaagaag agagggtcat ctccgacctc aagcgcttca tgaccaaggc cttcgacgac
    10081 gtcctggcca tgagcctcga gcacaaggtc aacatgcgca tcgccgcgtt catgctgggc
    10141 atccagcgcg tcacccgggc ggccgagatc cggggcctgt acgcgtaagg gcacccgcca
    10201 cgtcatgcac cttcggcccg tcgcgatcgg cctcgccgtc gcggcgggcc ttgtcttttc
    10261 cggttgctcg aagaagagcg acaccgccgg tggtggtgcc cctcgcgcga aacgcgcccc
    10321 gggctcgcca cccccctttg cactcccgcc caacaaccag ccggccacct acatcggcac
    10381 cttcgacgac acgccgagct ggtccgagcg ctggaccgtc tggctgcggc ccgactccac
    10441 ctacttcctg cgtgaggaac acgtcggtca tggagacgtg gccagcgatc attcggaaga
    10501 cgacatcggc cgatgggagc gggccacgga tggcgggccg atccgcctgc agggcgggcg
    10561 caatcagcct ttcgagttgg atgtgccgca tccggatacg cttcgattcc ggattgtcga
    10621 ccaggcgctg ggggtggaac cgcactttct tgtgcgaacc gacagcattg cgtatttcga
    10681 gccgcgggtg agtctcaccg gcactctgcg gctgacggcg gacgctgccc tgttcgacga
    10741 ctgcgcctcg agcttgcgat ggccggtatt gaaggaggag ggcttttccg cagcggaggt
    10801 cgcatatcgc cagtccgcgg tgaacccggg tgatgccctt ctcgtgtcga tcgagggaag
    10861 agttgttcgc cgaccaagga tgaacgatgg cccagcgcgg gattacctgg tcatcgagcg
    10921 attcggtcaa tggacaccag gaggccgatg tggggaaccc agtccagccg agggcctacc
    10981 gtacggcgct ggaaagtgac cacctccaac cactcccgcc gcatcgccgc catcgcaatc
    11041 gcttccatcg ccgcgggaca cacgctttac gcctgctcca agccggtcga tcggtcggag
    11101 cacgtgccca cagttcaggc gcgtcctgtc gcgtccgcat cgcgctccat ctttcccccc
    11161 gatcgcctgc cggccgcgta ctttggcaaa gtcgatctgc ccgctgggcg aagcgaacga
    11221 tggaccatct ggctgaagcc cgaactctat ttcctgcgcg tcgaataccg tgagaaggcc
    11281 ggattttcgt cggaagacat catcggcagg tgggacacga tcgcggatcg cacgattcga
    11341 ctccacgccg atggccggga tgtcatcgaa ctggaagtgc cggacctcga tacccttcga
    11401 ctcggagcag cagtcggtca acatggcacc aggccgcccg ccttgctcgc gcggaccagt
    11461 tcgatccact acctcgaacc gcacatcagc ctgaccggct acatgtcctg ctcggatgat
    11521 ggcggatact tccaggattg cggaacgggc tggggcttgc cgatcctcaa ggagggggat
    11581 ttcgaaacgg cgctgaggga gtatcacaat gtccggcccg atcggcggtc gccggagcag
    11641 gatcttatct ttgacagact tccgcgaccg tcgcagtcga cttcccgcgt cagcccccac
    11701 tcactggttg aatccgaatc ggcggggaag gtgatgcccc gcccgatgcc cgtgccccgc
    11761 ccgatgcccg tgcccccccg cgtcttcgtc tccctcgatg cccggatcgt cctgaggctg
    11821 agcgtgaacg gtgcctcggc cggcgaggcc ctggtcatcg agcgattcgg gcgcgtgatt
    11881 cgcggaaagg cgtgcgccga cacgtcggcc ctggcggaac gggaacggca ggagcgcctc
    11941 gcctcgcttc cctacgggat ccgatgacga acccggccga atactcccgc ctgatccggg
    12001 aggccgccct ccgccacggc ttccaccacg tcggcatcgc gcgagcccgg gaagtccggc
    12061 acgcggacga gttccgcgcc tggctggccg atggacgcca cgcctccatg agctacatgg
    12121 agaaacgagt tgacgagcgg ctcgacccgg agctcctcga cccggcgtac cagacgatcg
    12181 tgtcggtagc ctcaagctac ttccatgaca acgccgcgcc agcggcaacc gaaccggcgt
    12241 cctccctgcc tgcggggcgc atcgcccgct atgcgcacgg ggccgactac cacaatgtcc
    12301 tgggcaagca attgcgcaag ctcctcaagg agcttcgcat gtcattggat ggttgtgatg
    12361 gtcgggcctg ggtggacacc ggccccgtca tggatcgggc ctgggcgtct cgtgcgggga
    12421 tcgggtggtg ggggaagcac acgactctgg tgtcgcgcac ggatggttcg tggctcgttc
    12481 tcggcacgat ggcactcacg atcgagtgcg agccggatga gccgcacgag gatttctgcg
    12541 ggagttgtca tcggtgcatc gacgcgtgtc ccacgaaggc catcgtcgcc gagcgggagc
    12601 tcgacgcccg cttgtgcatc tcgtactgga cgatcgagca tcgtgggaca atttcggagg
    12661 cgatgcggcc ggccatcggc gattggatct tcggatgtga cctctgcctc gaggtctgtc
    12721 cctggaatcg atttgcgcag gccggtcgtg aggcaaggtt cgcggctcgc gaggatcgtg
    12781 cggcgccggc gctggagccg ctgctcgacc ttgacgatgc cgcgttccgg atcaagttca
    12841 acggcagccc gatccagcga gccacgcgtg acggcttcgt gcgcaatgtc tgtgtggcac
    12901 tgggaaacgt cggcacgcca cggaatgtgc gggcgctcga gcggattgcc ggcaacgacg
    12961 ccagcgaggt cgtgcgtgag cacgcgcact ggggaatcca acgaattcaa gaacgatcgg
    13021 gggagtagat gtccaggacg attctcgtga ccggtgccac cggggcccag ggtggctcgg
    13081 tggcgcatgc gttgttgacg catggcgggt tcaacgtgcg cgcgctgacg cgtcatccgg
    13141 attccgacaa ggcgcgggca ctgaaagccg cgggcgcggt gccggtggcc ggcgacctgg
    13201 ccgatcgtgc ttcgctcgaa gcagcccttg ccggctgctc ggcctgcttt ggggtcacca
    13261 acttctggga gcacttcgag gcggagttcg atcacggcaa gaatctcatc gaagcggtgg
    13321 ccgccgccgg cattcgcgac ttcctctaca gctcgctccc gcccatcgag aaatcgaccg
    13381 acggcaagct caaggcgccg cacttcgacc tgaaggccga actggaaacg ctggcgcgtc
    13441 ggctggtgcc gggctcggca ttcgtgcacc tgatgttcta ctacgaaaac ttcctcgcgg
    13501 gctccgcgcc gcagcggacg ccggatggca gctacgcctt tggctttccg cagggggatg
    13561 tgccgctggc cggcgtgtcg gccgaggatg tgggcggcgc catcgcgccg atcttcgccg
    13621 atttcgacgc gtggcgtggc aaggccattc ccatcgtggg cgacattctg ccgccggcgg
    13681 aatacgcggc catcctgtca cgggtgacgg gcgcgcgcgt cctgtacagc cacatcccgc
    13741 tcgaggtgtt ctccaagttc ccgttccccg gagcggacga cctcgccaac atgttcgagt
    13801 ggtatcgcct ctacatgccc gaccgtcacg cggcgtggga
//
LOCUS       DASVOD010000318         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_961577, whole genome shotgun sequence.
ACCESSION   DASVOD010000318 DASVOD010000000
VERSION     DASVOD010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961577"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3145
                     /locus_tag="VF720_07185"
     CDS             <1..3145
                     /locus_tag="VF720_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7879177.1"
                     /translation="IHSFCGQVLRERPIEAGITPGFGVADEVTAGTVFDEAWEEWLAT
                     ELADLHSPLKPVLEAGVGPGHLAALLERMRAHRDALREPDRVLESMAVAEARAEDAWK
                     EFRDAIGRLDAGLDELLATCRKSDDRAHLEMSTARAAVARLANRARGETEVPRHRLLG
                     DLPFGGFKNKGKKENWPDDCLIRLRTLATDATNAHKAWADHHRSMLTLLAVRRLLGAL
                     DAYARLKAERSLLDFDDLLLVTRDLLRRDATARRDLAGTWTRLLVDEFQDTDPVQVEI
                     VCLLAAAADAEVAEVLPVAAPGALFVVGDPKQSIYRFRRADIQMYQRTVRAMAESGSA
                     REVITENFRTRPSITAWVNRLFDRLMAEELSGDHQPSYAPLTPFRDEPPGDDGGVIHL
                     AIDGRETDADGATIDRSADERRRQEARGVAAWLKREIGSGNRTIVDREEGVVRPIRWS
                     DAAILFRTSTAVSFYEDALRRAEIPYRVEGGKFYFQRQEVQALLATLRAIDDPGNRVA
                     MVAALRSVLFGFTDDELVLAVEAGLFDAQPRGTPPRGIAAAVAWIRDCHARRHAEGPG
                     RMVEGVLGATQAIELFALRPLGEQRVANLRKIVDEARRFEGHAGGFREFIRFLSEREA
                     ALEKERDSPLVDDGDSGAVTLQTMHGSKGLEYGLVVLADLDGQFNTGRDSSYLKRDAE
                     GRVTLGFRLEGLIGGGGTTPGWDELKLDEEQHAAAERIRLFYVAATRARDRLVLPIAA
                     RDGKALGFRSLLETANLADLPLVSPPPEDSAEDEPLLRRGWRVPAPMDDGALAATQEV
                     QAARAAHRERLEALRSAWPPSARWFNPSLLGGGGKRATTNGDADADPVGAGPPSDGRP
                     VGRAVHGVLERVAAHGGDRMDRVAQALVAEGITDPDGGARVARFVDAALAHPLWARAR
                     AASWSGREVPFLAGWSAEEAGGAARRIDGICDLLFEEGDGLVLVDWKTDRHDADGWEG
                     AARRHRAQLLGYLLAIEAGAPRPVTELNLVFLEDGTTVSITVDDALRAEARRLYLAEG
                     VESPVGPAGSPVGPGGK"
     gene            complement(3148..5076)
                     /locus_tag="VF720_07190"
     CDS             complement(3148..5076)
                     /locus_tag="VF720_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879178.1"
                     /translation="MRHALIAAALIVATHVGVSPARAQGIFAGEVAGDDFGTGLAGVG
                     DLNNDGFEDFAIGAPGQDDVGSDAGRVYIFLGRAQNIGPVADIVLSSGDGGDHFGFAV
                     AGIGRFNNDAYDDMAVGSPDSDAGGSGRGRVHIYFGNSSGTSLTSGPILSGQQGGDQF
                     GYALDGGFDFNDDGRSDLAVGSPERPTGGARAGEVRIYFGASSPTASGDVILTGEQSQ
                     DWFGVSVSRGGDINNDGFGDLVVGAPQPFQSNAGKAYVIFGRSDGQPPVRLTLGGEFG
                     GDRFGWAVGGGGNLDNDAFDDVVVGAPKQDAGATQNGAAYVFRGGNPMNNVSDAKVAG
                     RESGDEFGSSLSIRGDWDDDNRSDLIVGAPLSDDGGNQAGEINLFLGASPINAGSRGV
                     YLGPRLLPGFAASDHFGTAVDFVDYNADGHSEVLAGSPRANQVSGDETGFASLTFSPG
                     SLVPVRLLGMTLVPGGDRITLDWRVESDGALLGFHVERRSGEAWRRVNANLLFEPTGH
                     YRFVDLDEDIEIGGNFAYRLVALGRGGLTDTFGPWTVAAGPGVRPFVDGAYPNPAPAG
                     VTVPIVMPESALAVVTIFDSAGRQVRRLHNGTLLKGRTTLWWDGTSDDGTRVPAGSYW
                     YRLETGRTAVSKKVVILR"
     gene            complement(5109..>5315)
                     /locus_tag="VF720_07195"
     CDS             complement(5109..>5315)
                     /locus_tag="VF720_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879179.1"
                     /translation="IAAGKTGFTRPAGYCLATHISAADNPTLTTVVLGAPSNSSRFSE
                     SAKLIDWAKKSLEPVAHDAAPSHR"
BASE COUNT          807 a         1753 c         1970 g          785 t
ORIGIN      
        1 catccactcg ttctgcggcc aggtgctgcg ggagcggccc atcgaggccg gcatcacgcc
       61 cggcttcggc gtggccgacg aggtgacggc cggcaccgtc ttcgacgagg cgtgggagga
      121 gtggctcgcg accgagctgg ccgatctcca ctctccgctg aagccggtcc tggaggccgg
      181 cgtcgggccc gggcacctgg cggcgctcct ggaacggatg cgcgcgcacc gcgacgcgct
      241 ccgcgagccc gaccgcgtgc tcgaatcgat ggcggtggcc gaagcgcggg ccgaggatgc
      301 ctggaaggaa ttccgcgacg cgatcggaag gctggatgcc gggctcgacg agcttcttgc
      361 cacctgccgg aagagtgacg acagggcgca cctggagatg tccacggcgc gcgccgcagt
      421 tgcgcggctg gccaaccggg cccgggggga aaccgaggtg ccccgacacc gcctgctcgg
      481 cgacctgccg ttcgggggat tcaagaacaa gggaaagaag gaaaactggc cggacgattg
      541 cctgattcgc ctccgcacgc tggccacgga cgccacgaat gcccacaagg cgtgggccga
      601 tcaccatcgg tcgatgctca cgctcctcgc cgtgcgccgg ctccttggcg cgctcgacgc
      661 ctacgcgcgc ctcaaggccg aacggagcct gctcgacttc gacgacctgc tcctcgtcac
      721 ccgcgacctg ttgcggcgtg atgccacggc gcggcgcgac ctggccggaa cctggacacg
      781 cctgttggtc gacgaattcc aggacaccga tccggtgcag gtggagatcg tctgcctcct
      841 ggccgcggcg gcggacgcgg aagtggcgga ggtgctcccg gtggccgcgc cgggcgcgct
      901 cttcgtggtg ggcgatccaa agcagtcgat ctaccggttc cgccgcgccg acatccagat
      961 gtaccagcgg acggtgcggg cgatggccga gtcgggaagc gcccgcgaag tcatcaccga
     1021 gaacttccgg acccggccgt cgatcacggc ctgggtcaat cgcctcttcg atcggctgat
     1081 ggccgaggag ctgtccggcg atcatcaacc gtcctacgcg cccctcaccc cttttcgtga
     1141 cgagccgccc ggggacgacg gaggggtgat ccacctggcg atcgatggtc gcgagacgga
     1201 tgccgatggg gcgaccatcg accggagcgc cgacgagagg cggcgccagg aagcccgcgg
     1261 cgtggcggcg tggctgaagc gtgaaatcgg gtcggggaat cggacgatcg tcgaccggga
     1321 ggaaggggtg gtgcgcccga tccgctggag cgatgccgcc atcctcttcc gcacctccac
     1381 cgcggtttcg ttctacgagg atgccctgcg acgggccgag atcccgtatc gcgtggaagg
     1441 ggggaagttc tacttccaga ggcaggaggt gcaggccctt ctcgccacgc tgcgcgcgat
     1501 cgacgaccct ggaaaccgcg tggccatggt ggccgcgctc cgatccgtgc tcttcggctt
     1561 caccgatgac gaactggtcc tggcggtcga ggccgggctg ttcgacgccc agccgagggg
     1621 aacgccgccg cgcggcatcg cggccgcggt ggcctggatt cgcgactgcc acgcgcgccg
     1681 gcatgccgag ggacccggcc ggatggtcga gggggtgctg ggcgccactc aggcgatcga
     1741 actcttcgcg cttcgcccgc tcggcgagca gcgcgtggcc aacctccgga agatcgtgga
     1801 cgaggcgcgc cggttcgaag gacatgccgg cggattccgc gagttcatcc gtttcctgtc
     1861 ggagcgggaa gccgcgctgg agaaggagcg ggactcgccg ctcgtcgacg acggcgattc
     1921 gggcgccgtg acgctgcaaa cgatgcacgg ctcgaagggg ctcgagtacg gtctcgtcgt
     1981 gctggccgat ctcgacggac agttcaacac cggacgggac agctcgtacc tgaagcgcga
     2041 tgccgagggt cgggtgacgc tcggcttccg gctggaggga ctcatcggcg gcggcggcac
     2101 gacaccggga tgggacgaat tgaagttgga cgaggagcag catgccgcgg ccgaacgcat
     2161 ccggctcttc tacgtggcgg ccacccgcgc ccgggatcgt ctcgtacttc ccatcgctgc
     2221 gcgtgacggc aaggcgctgg gattccggtc gctcctcgag acggccaacc tggccgacct
     2281 gccgctcgtc agtccgccgc ccgaagattc ggcggaagac gaaccactgc tgcgccgtgg
     2341 gtggcgcgtt ccggcaccga tggacgatgg ggcgctggcg gccacacagg aggtgcaggc
     2401 ggcccgcgcc gcccaccgcg agcggctgga agcactccga tcggcctggc cgccgtcggc
     2461 acgctggttc aatccctcgc tcctgggcgg cggtggcaag cgggcaacca ccaacgggga
     2521 tgcggacgcc gatccggttg gcgccggacc tccttccgat ggacgacccg tggggcgggc
     2581 ggtgcacggt gtgctcgagc gggtggcggc ccatggtggt gaccggatgg atcgggtcgc
     2641 gcaggcgctc gtggccgagg ggatcaccga ccccgacggg ggggcgaggg tggcgcgatt
     2701 cgtcgatgcg gccctcgcgc atcccctctg ggcacgcgcg cgagcggcgt catggagtgg
     2761 acgcgaggta ccgttcctgg ccggctggtc ggcggaagag gcgggcggag cagcgcgccg
     2821 gatcgacggg atctgcgacc tgctcttcga ggaaggggac ggactcgtcc tcgttgactg
     2881 gaagaccgat cgccacgatg cggatgggtg ggaaggggcg gcgcgacggc atcgcgcgca
     2941 gttgctcggt tatctcctgg cgatcgaggc gggcgcgccg cgtcccgtca ccgagttgaa
     3001 cttggtcttc ctggaagacg gcacgacggt atcgatcacg gtggacgacg ccctgcgtgc
     3061 cgaggcgcgg cggctttacc tggcggaagg ggtggagagc ccggtcggcc cggcggggag
     3121 tcccgtcggc ccgggcggga agtgacgtca gcgaaggatg acgaccttct tgctgaccgc
     3181 ggtccgcccc gtctcgagtc ggtaccagta gctccccgcc ggaactcggg ttccgtcgtc
     3241 gctcgtgccg tcccaccaga gcgtggtccg tcccttgagc agcgtgccgt tgtgcaggcg
     3301 acgcacctgg cgtccggccg agtcgaagat cgtgacgacg gccagcgcgc tttccggcat
     3361 gacgattgga accgtgacgc cggccggggc cgggttggga taggcgccgt ccacgaacgg
     3421 ccggacgccg ggcccggcgg ccaccgtcca gggtccgaag gtgtcggtga gcccgccgcg
     3481 cccgagggcg acaagtcgat acgcgaagtt gccgccgatc tcgatgtcct cgtccaggtc
     3541 gacgaaccga tagtggcccg ttggctcgaa gagaagattg gcgttgaccc ggcgccaggc
     3601 ctctccggac cgacgctcca catggaagcc gaggagcgca ccatcgctct cgacccgcca
     3661 gtcgagcgtg atccgatcgc ctcccgggac gagcgtcatg ccgaggaggc gcaccggcac
     3721 cagcgagccc ggggaaaagg tgagcgaggc gaagccggtt tcgtcgccgc tcacctggtt
     3781 ggcgcgcggg gagccggcca gcacttccga gtggccatcc gcgttgtagt cgacgaagtc
     3841 gaccgcggtg ccgaagtggt cgctggccgc gaagccgggg aggagccggg ggccgaggta
     3901 gacgccgcgg ctgccggcgt tgatcggcga ggcgccgagg aagaggttga tctcgccggc
     3961 ctggttgccg ccgtcgtccg aaaggggcgc gccgacgatg aggtcggagc ggttgtcgtc
     4021 gtcccagtcg ccgcggatgg agaggctcga accgaattca tcgcccgatt cgcggccggc
     4081 caccttggcg tcggagacgt tgttcatcgg attgcccccg cggaacacgt aggcggcgcc
     4141 gttctgcgtg gccccggcat cctgcttcgg cgcgccgacc accacgtcat cgaaggcgtc
     4201 gttgtcgagg ttgccgccgc cgcccaccgc ccagccgaag cggtcgccgc cgaactcgcc
     4261 gccgagcgtg aggcgcacgg gaggctggcc gtcggaacgg ccgaagatca cgtacgcctt
     4321 gccggcattg gattggaacg gttgcggggc gcccacgacc aggtcgccga atccgtcgtt
     4381 gttgatgtcg ccgccccggc tgaccgaaac gccgaaccag tcctgcgact gctcgcccgt
     4441 caggatgaca tcgcccgacg ccgtcgggct cgaggcgccg aaatagatgc gcacctcacc
     4501 ggccctcgcg ccgccggtgg ggcgctcggg gctgcccacg gccaggtcgc tccggccgtc
     4561 gtcgttgaag tcgaagccgc cgtcgagggc atacccgaac tgatcgcccc cctgctggcc
     4621 ggagaggatg gggccggagg tcaggctggt gccggaggaa ttgccgaagt agatgtggac
     4681 gcgcccgcgt cccgagcctc cggcgtcgct gtcgggggag ccgacggcca tgtcgtcgta
     4741 ggcgtcgttg ttgaaacggc ctatgccggc cacggcaaag ccgaagtgat cgcctccgtc
     4801 gccgctggaa aggacgatgt cggccaccgg cccgatgttc tgtgcgcggc cgaggaagat
     4861 gtagacgcgg ccggcgtcgc tacccacgtc atcctggccc ggtgcgccga tcgcgaagtc
     4921 ctcgaagccg tcgttgttga ggtcgcccac gccggcgagg cccgtcccga aatcatcgcc
     4981 ggccacttca ccggcaaaga tcccctgggc ccgggcggga gaaaccccca cgtgcgtggc
     5041 gacgatcagg gcggcggcga tcagtgcgtg acgcatggat ccacctcgtt cgaaggagga
     5101 gcgacgattc agcgatgcga gggtgcggcg tcgtgcgcga cgggctccag gctcttcttg
     5161 gcccagtcga tcagcttggc cgactcggag aagcggctcg aattggacgg ggcgcccagg
     5221 accacggtgg tcagggtggg gttgtcggcg gcgctgatgt gcgtggcgag gcaatagccg
     5281 gccggccggg tgaatcccgt tttcccggcc gcgat
//
LOCUS       DASVOD010000319         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_804287, whole genome shotgun sequence.
ACCESSION   DASVOD010000319 DASVOD010000000
VERSION     DASVOD010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804287"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF720_07200"
     CDS             <1..240
                     /locus_tag="VF720_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879180.1"
                     /translation="ASRYTFTTIADVEGRFRFEKLMPGEYYVTSSLSLSSPRVHDSGI
                     GGKYTDEHTKTAVVLARVRITAGEAKTLRMSPKGK"
     gene            245..1177
                     /locus_tag="VF720_07205"
     CDS             245..1177
                     /locus_tag="VF720_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016395091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX7879181.1"
                     /translation="MRLTGGFRECGALLLLCLLHLALATPSMAQDIEPRSYSNAPVGV
                     NFLITGYIYTNGGLAFDSASPIQNPKLTTSSAVAAYARVLGIAGRSAKCDVIVPYSWL
                     DGSADYQGERVTREVSGLMDPRVRFSVNLLGAPALSLKEFAGYRQNVILGASVQASVP
                     LGQYDPARLVNLGSNRWFVKPELGISKHIGRLTLEGAAAAILFSDNDEFYVGRTRSQD
                     PLYSFQGHVVYGFRSGIWGAFDANYFTGGQVTLDGVANDDRQENWRLGGVLTFPVNRR
                     NSVKIHGSSGVSARTGNNFDAAGIAWQFRWGGGL"
     gene            complement(1192..>2289)
                     /locus_tag="VF720_07210"
     CDS             complement(1192..>2289)
                     /locus_tag="VF720_07210"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7879182.1"
                     /translation="FDLGFTTLRLGYGLVWDFVSHDQDEESKEQITTENKNDLRDFRF
                     LFKGKFKTKRPLSWTMGVMYDGPTEDWLFRQTGLMVGVPEISSDFFIGRTKEGYSQYK
                     VMVGYDLWTIERSFFNDAFIPILADGIKWSGHVPRAHLTWNLGYYADVVSEDEKFSTY
                     DDQFVGRFIVYPVLNEQQVLHLAVMGRDVTPDEGSFRARSRPESFLSPYFVDTGAFET
                     DHARTFGLEGYYRNRSWLVGGEYGIQNFDAPASGDPTFTGGNLSVVWLPTGETRGYNS
                     AGGFFKTIIPRKTVFEGGPGAIELSLNYSWIDLDDGALHGGTMWRVSPSVSWHLMDYT
                     RIQFAYGYGVLDRFDLEGTTQFFQLRLFTGL"
BASE COUNT          414 a          694 c          761 g          420 t
ORIGIN      
        1 gccagccggt acacgttcac gacgatcgcg gacgtcgaag gacgattccg attcgagaag
       61 ttgatgcccg gcgagtacta cgtgacgtcc agcctctcgt tgagcagccc gcgggtgcat
      121 gatagtggca ttggtgggaa atacaccgac gagcatacga aaacggccgt ggttcttgcg
      181 cgtgtccgga tcaccgcggg tgaagcaaag acgctccgga tgagcccgaa ggggaagtga
      241 ttcgatgcgg ctgaccgggg gcttccgaga gtgcggcgct ctccttcttc tctgtctcct
      301 gcatcttgcc ctggcaacgc cctcgatggc ccaggacatc gagccccgca gctactccaa
      361 cgctccggtc ggcgtgaatt tcctgatcac cgggtacatc tacacgaatg gcggcctggc
      421 gttcgacagc gcctctccca tccagaaccc gaagttgacg acctcgagcg ccgtggccgc
      481 gtacgcccgc gttctcggta tcgccgggcg atcggccaag tgcgatgtca tcgtgccgta
      541 ctcatggctg gacggctccg ccgactacca gggggagcgg gtcacacggg aggtgtccgg
      601 cctcatggat ccgcgggtcc ggttctccgt gaacctgctc ggggcaccgg cgctttcact
      661 gaaggagttc gcgggttacc gacagaacgt gatcctcggg gccagcgtcc aggcgtcggt
      721 gccgctgggc cagtacgatc ccgcgcggct ggtgaacctg gggagcaacc gatggttcgt
      781 gaagccggag ttgggaatct cgaagcacat cgggcgactg acgctggaag gggcggcggc
      841 ggccatcctg ttcagcgaca acgacgagtt ctacgtgggg cgcacgcgat cgcaggatcc
      901 cctgtactcc ttccagggtc acgtggtgta cggctttcga tccggaatct ggggcgcgtt
      961 cgacgccaac tacttcacgg gcggccaggt aacgctcgac ggcgtggcga atgacgaccg
     1021 ccaggaaaac tggcgcctcg ggggtgtgct gacctttccg gtcaaccggc ggaattccgt
     1081 gaagatccat ggcagcagcg gcgtatcggc ccgcaccggg aacaacttcg acgcggcggg
     1141 aatcgcctgg cagttccggt ggggcggtgg gctctaggcc ggccgcctgc cttacagccc
     1201 cgtgaacaac cgcagctgga agaactgcgt ggtgccttcg aggtcgaagc ggtcgaggac
     1261 cccgtagccg taggcgaact gaatgcgcgt gtagtccatc aggtgccagc tgaccgacgg
     1321 gctgacgcgc cacatcgtgc cgccgtgaag cgcgccgtcg tccaggtcga tccaggagta
     1381 gttcagcgac agctcgatcg cgcccggccc accctcgaag acggtcttcc tggggatgat
     1441 cgtcttgaag aagcctccgg ccgagttgta gccgcgggtt tcgccggtgg ggagccacac
     1501 gacggagaga ttgccgccgg taaaggtggg gtcgccggag gcaggtgcgt cgaagttctg
     1561 gatgccgtat tcaccgccga ccagccagga cctgtttcgg tagtacccct cgaggccgaa
     1621 ggtgcgggcg tgatccgtct cgaaagcgcc ggtgtcgacg aagtagggtg acaggaacga
     1681 ctcggggcgg gatcgggccc ggaacgatcc ttcatccggg gtgacatcgc gtcccatcac
     1741 cgccaggtgc aggacctgct gttcattcaa caccgggtac acgatgaagc ggccgacgaa
     1801 ctggtcgtca tacgtggaga acttttcgtc ctccgacacg acgtcggcgt agtagccgag
     1861 gttccaggtc aggtgggcgc gcggcacgtg gccgctccac ttgatgccgt ccgccaggat
     1921 cgggatgaac gcgtcattga agaagcttcg ctcgatggtc cagaggtcat aaccgaccat
     1981 aaccttgtat tgcgaatagc cttccttggt ccgtccgatg aagaagtcgc tggagatttc
     2041 cggcacgccc accatgaggc ccgtctggcg gaagagccag tcttccgtgg ggccgtcgta
     2101 catcacgccc atcgtccagc tgagggggcg cttcgtcttg aactttccct tgaagaggaa
     2161 gcggaagtcg cgcaggtcgt tcttgttctc ggtggtgatc tgctccttgc tctcctcgtc
     2221 ctggtcatgc gacacgaaat cccacaccag gccgtaaccg aggcggagcg tcgtgaagcc
     2281 gaggtcgaa
//
LOCUS       DASVOD010000320         1648 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_751794, whole genome shotgun sequence.
ACCESSION   DASVOD010000320 DASVOD010000000
VERSION     DASVOD010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751794"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..323
                     /locus_tag="VF720_07215"
     CDS             27..323
                     /locus_tag="VF720_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEX7879183.1"
                     /translation="MTTETLDRDQLRAKVQELLDDMINPAVAGHGGVIHLVDVKDDWV
                     FLHMGGGCQGCGMADATLRQGIETMLKEEIPQIKGIVDQTNHAEGETPFYPGAE"
     gene            330..1367
                     /locus_tag="VF720_07220"
     CDS             330..1367
                     /locus_tag="VF720_07220"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879184.1"
                     /translation="MRQAPSMDERPPDARVDPEGDTVVPPSAVDPDADTLDRSETPSS
                     VSVSQPVTAKLTQPIGLKTGALPSTIGRFRVRRIIGSGGMGIVFEAHDPEFNRSVAIK
                     VIRQRVASPATLRRFEDEARLLARLQHPSIAAIYDAGRHEDEGLVLPYLALEFVPAAR
                     VLTRYANEEKLDLTARLKLVVAIARAVEYGHGQGVVHRDLKPGNVLVGRDGTLKIIDF
                     GVAHATGPAEPDQGEARRGLVGTLAYMSPEQCEGDPARIDARSDVYSIGALTYELLVG
                     QVPVEVRGLAVGPAVKKIRGTAPRAPSAANAEVPASLDPVLLRALSKSRSQRYPSAAE
                     LADELTNWLSA"
     gene            1384..>1648
                     /locus_tag="VF720_07225"
     CDS             1384..>1648
                     /locus_tag="VF720_07225"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7879185.1"
                     /translation="MNLFVSTTVSGRRQVWPLEGTILEVGRSSKCAIQLADGTVSKVH
                     SEFSRAGDGWLIRDLGSRNGTRLNGQPVAEPKALKPGDKVEIGQ"
BASE COUNT          286 a          581 c          549 g          232 t
ORIGIN      
        1 agccggcccc ggccggagga gttcacatga ccaccgaaac tctcgatcgc gaccagctcc
       61 gggcaaaggt gcaggagctg ctggacgaca tgatcaaccc ggccgtggcg ggtcacggcg
      121 gcgtcatcca cctcgtcgat gtcaaggatg actgggtctt cctgcacatg gggggcggct
      181 gccaggggtg cggcatggcc gatgccacgc tccgccaggg gatcgaaacg atgctgaaag
      241 aggaaatccc gcagatcaag gggatcgtcg accagacgaa ccacgccgaa ggtgaaactc
      301 ccttctaccc cggcgccgag tagacgacct tgaggcaggc accctcgatg gacgagcgtc
      361 cccccgacgc gcgggtcgat cccgaggggg acaccgtcgt cccaccgtcc gcggtcgacc
      421 cggacgccga cacgctcgat cggtccgaga cgccgtcgtc cgtaagcgtc tcgcagccgg
      481 tcacggccaa actgacgcaa cctatcggcc tcaagacggg ggcgctcccg tccaccatcg
      541 ggcggttccg ggtgcggcgg atcatcggct cgggcggcat gggaatcgtc ttcgaggctc
      601 acgacccgga gttcaaccgc tcggtggcca taaaggtcat ccgccagcgg gtggcctcgc
      661 cggccacgct ccggcgattc gaggacgagg cgcgcctcct ggcccggttg cagcacccgt
      721 ccatcgcggc catctacgac gccgggcgtc acgaggacga aggcctggtc ctcccgtacc
      781 tggcactcga attcgtgccg gccgcccgcg tccttacccg gtatgccaac gaggaaaaac
      841 tcgacctcac cgccaggttg aagctcgtcg ttgcgatcgc ccgggcggtg gaatatgggc
      901 acggacaggg tgtggtccac cgcgacctga agcccggcaa cgtgctggtc gggcgcgacg
      961 gcacgctcaa aatcatcgac tttggcgtgg ctcatgccac cggcccggct gagccggacc
     1021 agggagaggc ccgtcggggc ctcgtgggca cgctggccta catgagcccg gaacagtgcg
     1081 aaggcgatcc ggcccgcatc gacgcccgca gcgacgtcta ctccatcggc gcgttgacct
     1141 acgaactcct cgtcggccag gtgccggtgg aggtgcgcgg gctggcggtg ggccccgccg
     1201 tcaagaagat ccgcggaacg gcgcccaggg ccccatccgc ggccaatgcc gaggtgcccg
     1261 cctcgctcga ccccgtcctg ctgcgcgcgc tttccaagtc gcgcagccag cgttacccgt
     1321 ccgcggccga actggccgac gaattgacca actggctctc cgcctgagga tccggccgca
     1381 cccatgaacc tgttcgtcag cactaccgtc agcgggcgcc gccaggtgtg gccgctcgag
     1441 gggacgatcc tcgaagtggg ccgatcctcc aagtgcgcca tccagctggc cgacggcacc
     1501 gtctcgaaag tccactcgga attctcgcgc gccggcgacg gctggctcat ccgcgatctc
     1561 ggcagccgca acggcacgcg cctgaacggc cagccggtgg cggaacccaa ggccctgaag
     1621 ccgggcgaca aggtggagat cgggcagg
//
LOCUS       DASVOD010000321         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_440301, whole genome shotgun sequence.
ACCESSION   DASVOD010000321 DASVOD010000000
VERSION     DASVOD010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440301"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF720_07230"
     CDS             complement(<1..216)
                     /locus_tag="VF720_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879186.1"
                     /translation="MEAIPGEIRPKAPLLARLIATVGYTGFFPWAPATLASLIVSTIY
                     FVLPPMALWVQAVALVLVTLIGIITATM"
     gene            complement(216..818)
                     /gene="recR"
                     /locus_tag="VF720_07235"
     CDS             complement(216..818)
                     /gene="recR"
                     /locus_tag="VF720_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX7879187.1"
                     /translation="MQYSSRIVEQLVEELARLPGIGRKTAQRLAFHLLRTSREEALRL
                     SDAVRNVKDKVDACTICGNLTEEDPCLICRDARREKDVICVVETPVDVVAVEQSGVYR
                     GQYHVLGGAINPLDNVGPDDIRAHQLVERVRAQGTREVILATNPNPAGEATAGYLADL
                     LAPTGVSVTRIARGVPVGSDIEYSDQATLARAMEGRKGMS"
     gene            complement(826..1137)
                     /locus_tag="VF720_07240"
     CDS             complement(826..1137)
                     /locus_tag="VF720_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018248331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX7879188.1"
                     /translation="MKFNSMMKQAQAMQNKMAKMQEELAEMVIEGTAGGGVVTVTMNG
                     KQEIQSIKIKPEAVDPEDVEGLEDLLMAAYNDAKGRASELMEEHMKKATGGLGLPPGL
                     F"
     gene            complement(1134..>1638)
                     /locus_tag="VF720_07245"
     CDS             complement(1134..>1638)
                     /locus_tag="VF720_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879189.1"
                     /translation="PAARTGAPAPRPTAPTPSAPAPQPPGEPMAAAPPPSTDIPSDPG
                     ALWTKTIAAVGAKKRSLGAFLAGSRAVGIDPNGRLVIEPAANDPMSREHLEDGDNRRF
                     IEQVLREITGQPVGYLVNAPARPASGGDTGSGGAPESAPRSPFELAADHPIVQKALDL
                     FDGEITS"
BASE COUNT          235 a          553 c          558 g          292 t
ORIGIN      
        1 catggtggcg gtgatgatgc cgatgagcgt gacgaggacc agcgccacgg cctgcaccca
       61 gagcgccatc ggcggcagca cgaaatagat agtcgacacg atcaggctgg ccagcgtggc
      121 cggcgcccag ggaaagaatc ccgtgtagcc gacggtggcg atcaaccgcg ccagcagcgg
      181 cgccttcggc cggatctcgc cgggaatggc ctccatcacg acatcccctt gcgcccttcc
      241 atggcacggg ccagcgtagc ctggtcggag tattcgatgt cgcttcccac cggcacgccg
      301 cgggcgatgc gcgtgaccga cacgccggtc ggcgccagga ggtcggccag gtagccggcc
      361 gtggcctccc ccgccgggtt gggattggtg gccaggatca cctcgcgcgt tccctgcgcg
      421 cgcacgcgtt ccacgagctg gtgcgcgcgg atgtcgtcgg gacctacgtt gtcgagcgga
      481 ttgatggcgc cgccgagcac gtgatactgc ccgcggtaca cgcccgactg ctccaccgcc
      541 accacgtcga ccggcgtttc caccacgcag atcacatcct tctcgcgccg cgcgtcacgg
      601 cagatgaggc agggatcttc ctcggtgagg ttgccgcaga tggtgcaggc gtccaccttg
      661 tccttgacgt tgcgcaccgc gtccgaaagg cggagcgcct cctcgcgcga ggtgcggagc
      721 aggtggaagg ccagccgctg ggccgtcttt cgaccgatgc cgggaaggcg ggccagttcc
      781 tcgacgagct gttcgacgat gcggctggaa tactgcatcc ctgtcctaga aaaggccggg
      841 cgggaggccg agtccgccgg tggccttctt catgtgctct tccatgagct ccgacgcacg
      901 ccccttggcg tcgttgtacg ccgccatcag gagatcctcg agcccttcca cgtcttccgg
      961 gtcgacggct tcgggcttga tcttgatcga ctggatctcc tgcttgccgt tcatcgtgac
     1021 ggtcacgacg ccgccgccgg ccgtcccctc gatcaccatt tccgcaagtt cttcctgcat
     1081 cttggccatc ttgttctgca tggcctgggc ctgcttcatc atgctgttga atttcatgac
     1141 gtgatctctc cgtcgaagag gtcgagtgcc ttctggacga tggggtggtc cgcggccagt
     1201 tcgaagggcg agcgcggggc cgactcgggc gcccccccgc tccccgtgtc gccgcccgac
     1261 gccgggcgcg ccggggcatt caccaggtag ccgaccggtt ggccggtgat ctcgcgcaac
     1321 acctgttcga tgaatcggcg attgtcgcca tcctcgagat gctcgcgcga catggggtcg
     1381 ttggcggcgg gttcgatcac cagtcttcca ttgggatcga tgccgacggc ccggctcccc
     1441 gccaggaacg cgccgaggga gcgcttcttc gcacccacgg ccgcaatggt cttcgtccag
     1501 agcgcgcccg gatcggaggg gatgtctgtc gacggcggcg gtgcggccgc catcggctca
     1561 ccgggaggtt gcggcgcggg cgcggacgga gtcggggcag tgggtcgcgg agccggagcg
     1621 ccagtccgtg ccgcaggg
//
LOCUS       DASVOD010000322         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_962244, whole genome shotgun sequence.
ACCESSION   DASVOD010000322 DASVOD010000000
VERSION     DASVOD010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962244"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VF720_07250"
     CDS             <1..436
                     /locus_tag="VF720_07250"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879190.1"
                     /translation="DVGPPDGVSLLRDPRHDRLVAVRNQPGKPIDTWFLSFTRSPVDP
                     DAAARGTVWRIGPARVTAAGDGIEIPFQLTAATRVIGAVHDLEGKLVRRLDEAKLPAG
                     SWTFVWDGRDESGVSVPPGPYDVMLTRLDTGAVGKLRVAREE"
     gene            complement(523..1008)
                     /locus_tag="VF720_07255"
     CDS             complement(523..1008)
                     /locus_tag="VF720_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX7879191.1"
                     /translation="MSDRGASDNGRRKFLSGLAVGLSGAIGAALAGIGLNTFLCPALA
                     SSDPRWIRVGEVDPDRISGPRGFVVSFRQSDGWFKGSGQGVVYVTKGADEQIKVLAST
                     CTHLGCGVRWDQAKERFLCPCHGGEYDANGAVTAGPPPRPLTPLESRFEDGVLYVKGL
                     A"
     gene            complement(1019..3142)
                     /locus_tag="VF720_07260"
     CDS             complement(1019..3142)
                     /locus_tag="VF720_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEX7879192.1"
                     /translation="MMARATNTRNKPGTALTAEQLVGAYRTMYLSRRLDDAEIRLKKQ
                     NQTFFQISGAGHEAFLVATGLCMKGGYDWFYPYYRDRALCLAIGVTPTEMLYQAVAAA
                     DDPASGGRQMPSHWGHKKWNLVSQSSPTGTQLLQAVGCAEAGMRYGLIPEIADREKHF
                     HKDEVVYVSVGDGTMSQGEYWEALNTACVLKLPVIFMLEDNGYAISVPSTVQTPGGDW
                     SKTVSGFENLFRIKFNGNDPIESYDAISQAVNYCRARKGPAFVHATCTRPYSHSMSDD
                     QAAYRSKSEREEEARHDCLITFAAYLVDHGILTAEELESLKASVNDQIESAVEEAIAA
                     AKPASGSAYDFLYSPDVDPTTAAFDRAPAGTGEPVSMVDNINKCLHDELARDPSIVVF
                     GEDVADATFEETLKEVAGKGGVFKLTHGLQRRFGGHRVYNSPLAEANIIGRAVGLATR
                     GLKPVVEIQFFDYIWPAMMQLRNELAMMRWRSNNNFKSPVVVRVPIGGYLQGGSVYHS
                     QSGESIFAHCPGLRIVMPSNGADAAMLLRTAIRCDDPVLFLEPKTLYRQPFSKSVYPG
                     PEAMIPLGKARTVRPGSDMTIVTYGSLVRRSMMAAEKAAEEQGIEVEVIDLRSLVPWD
                     KEAVYASVKKTGRVIVAHEDQLSWGFGAEIAARIGEELFEHLDAPVQRIGALDVPVAY
                     CPPIEDEILPQQEWVAAAIRKLAAY"
     gene            3362..>4290
                     /locus_tag="VF720_07265"
     CDS             3362..>4290
                     /locus_tag="VF720_07265"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7879193.1"
                     /translation="MTRSPGTARVWPPPAPQLLFVILAVILPGLAPSARAQTILPPDF
                     VETSIVENLGQPQTGFAFLPDGRWLVVDKPGRVRVVNTAGQHAIIHTLGNVYTTGEAG
                     LAGIAVDPSWPTKPFVYLHYNQNTPHRLIVARYRATGNLTDPTGLTMALSDPYVILDV
                     DDSSPNHNGSCLRFGPSGLLYVSLGDDVFSCVAQDSTLLKGVIARITVANLPPTGPGP
                     APRSLILPPGNPFAPDTTDAELVWCYGLRNPYRFTVDPYTEELYIGDVGGTEFDEADV
                     AVGGENFGWPILEANDSTGVWCFQGLPKFTDPIL"
BASE COUNT          699 a         1453 c         1418 g          720 t
ORIGIN      
        1 tgatgtcggc ccgcccgacg gcgtgagcct cctccgcgat ccgcgccacg accggctggt
       61 ggccgtccgc aaccagcccg gcaaaccgat cgacacctgg ttcctgtcgt tcacgcgcag
      121 cccggtcgat ccggatgccg ccgcgcgcgg caccgtctgg cgcatcggcc ccgcgcgtgt
      181 caccgccgcc ggcgacggca tcgagatccc gttccagctc accgccgcca ctcgtgtcat
      241 cggcgcggtc cacgatctcg agggaaagct ggtgcgccgt ctcgacgaag caaagctccc
      301 ggccggttcg tggactttcg tgtgggatgg ccgcgacgag tccggagttt cggtgccgcc
      361 gggtccctac gatgtcatgc tcacgcggct cgataccggc gcggtgggga agctgagggt
      421 ggcgagggag gagtagggga gctcggcgat cagggcagca atgaatgaca aagtgcccgg
      481 gggaaaggag atcatcgatg tggcccgggc gtcaggcgga gcctaggcca accccttcac
      541 atacagcact ccatcctcga accggctctc gagtggcgtg agcggcctcg gcggcggtcc
      601 cgccgtgacg gcaccgttgg cgtcgtactc acccccatga cacggacaga ggaagcgttc
      661 cttcgcctgg tcccagcgga ccccgcaccc gagatgcgtg caggtcgagg ccagcacctt
      721 gatctgctcg tcggcgccct tggtcacgta aacgacgccc tgcccgctcc ccttgaacca
      781 gccgtcggac tggcgaaagg agacgacgaa gccgcggggt cccgaaatgc ggtcagggtc
      841 gacctcgccg acgcggatcc agcgcgggtc gctcgaggca agcgcggggc agaggaacgt
      901 gttcaggccg atgccggcca gcgcggcgcc gatggctccg gagagtccca cggccaatcc
      961 ggaaaggaac ttccgccggc cgttgtccga ggcgccgcgg tcgctcatcg acgtgccgtc
     1021 agtaggccgc gagtttccgg atggccgccg ccacccactc ctgctggggg aggatttcgt
     1081 cttcaatagg ggggcagtag gccaccggaa cgtccagcgc accgatgcgc tggacggggg
     1141 catcgaggtg ctcgaacagt tcctcgccga tccgggcggc gatctcggca ccgaatcccc
     1201 acgacagctg gtcctcgtgg gccacgatga cgcggccagt cttcttgacc gaggcgtaca
     1261 ccgcctcctt gtcccacggc acgaggctgc gcaggtcgat gacctcgacc tcgatcccct
     1321 gctcctcggc ggctttttcg gcggccatca tcgagcggcg cacgagcgag ccgtaggtca
     1381 cgatcgtcat gtcggagccg ggacgcacgg tgcgcgcctt cccgagcggg atcatggcct
     1441 ccgggcccgg atagaccgac ttggagaaag gctggcgata gagcgtcttg ggctcgagga
     1501 agagcaccgg atcgtcgcaa cggatggcgg tgcgcaggag catcgcggca tcggcgccgt
     1561 tcgacggcat cacgatgcgg agccccgggc agtgggcgaa gatcgattcg cccgactggc
     1621 tgtggtacac ggaaccaccc tgcaggtaac cgccgatcgg cacgcgcacc accaccgggc
     1681 tcttgaagtt gttgttcgag cgccagcgca tcatggccag ctcgttccgg agctgcatca
     1741 tggcaggcca gatgtagtcg aagaactgga tttccacgac cggcttcaac ccgcgcgtgg
     1801 ccagccccac cgcgcggccg atgatgttgg cttcggccag cggcgagttg tacacgcgat
     1861 ggccgccgaa ccggcgctgc aggccgtggg tgagcttgaa cacgcccccc ttgccggcca
     1921 cctccttgag cgtttcctcg aacgtggcgt cggccacgtc ctcgccgaac accacgatcg
     1981 acgggtcacg ggccagctcg tcgtggaggc acttgttgat gttgtcgacc atcgacaccg
     2041 gttcaccggt gccggcgggc gcgcggtcga acgcggccgt ggtcggatcg acatcgggcg
     2101 agtagagaaa gtcgtacgcc gagccggagg ccggcttggc cgccgcgatg gcttcctcga
     2161 cggccgattc gatctggtcg ttcaccgagg ccttgaggct ttccagttcc tcggcggtca
     2221 ggataccgtg gtcgacgagg tacgccgcaa acgtgatgag gcagtcgtgc ctggcctctt
     2281 cctcgcgctc gctcttcgag cgataggccg cctggtcgtc cgacatggaa tggctgtagg
     2341 ggcgggtgca ggtggcgtgc acgaaggcgg gccccttgcg ggctcggcag tagttcaccg
     2401 cctgcgagat ggcgtcgtac gattcgatcg gatcgttgcc gttgaacttg atgcggaaga
     2461 gattctcgaa tccggagacg gtcttgctcc agtcgccgcc cggtgtctgc acggtggatg
     2521 gcaccgagat ggcgtagccg ttgtcctcga gcatgaagat cacggggagc ttgagcacgc
     2581 aggccgtgtt cagcgcctcc cagtactccc cctggctcat cgtgccgtcg ccgaccgaca
     2641 cgtacaccac ttcatccttg tggaagtgct tttcgcgatc ggcgatctcc gggatcaggc
     2701 cgtagcgcat ccccgcctcg gcgcaaccga ccgcctgcag gagctgcgtg ccggtggggc
     2761 tcgactggct gaccaggttc cacttcttgt ggccccagtg gctcggcatc tgccgaccgc
     2821 ccgaggcggg gtcgtccgcc gcggccaccg cctggtagag catctccgtg ggcgtgacgc
     2881 cgatggccag gcagagcgcg cgatcgcgat agtagggata gaaccagtcg tagccaccct
     2941 tcatgcagag gccggtggcc acgaggaacg cctcgtgtcc ggcaccggaa atctggaaga
     3001 aggtctggtt ctgcttcttg aggcggatct cggcgtcgtc gaggcgccgg gagagataca
     3061 tcgtgcggta ggcgccgacc agctgctcgg ccgtgagggc ggtacctggc ttgttcctgg
     3121 tattggtggc acgcgccatc atcggactcc agccggaggg ggggaagtca tccaaagaga
     3181 tcgttgcgag ccgctaattt accgcgacgg ccccggggcg ggcaagacga acccagggcc
     3241 cgcccggaat cgcccggagt ggtgcttggc ggtggtgggg gtggtaccct acgatcccgc
     3301 aaccggcaag ggggcctgag tccggtttgc atcattgacc gccgccgaga ggaaaccgcc
     3361 catgactcga tccccgggca cggcccgagt gtggcctccg cctgccccgc aacttctctt
     3421 cgtcatcctc gccgtcattc tcccaggact tgcaccctcc gcgcgcgccc agaccatcct
     3481 ccctcccgac ttcgtcgaga cgagcatcgt cgagaacctt ggacagccgc agaccggatt
     3541 tgcgttcctg cccgacgggc gctggctcgt ggtcgacaag ccgggacggg tgcgcgtggt
     3601 caacaccgcc ggccagcacg ccatcatcca cacgctcggg aacgtctaca ccaccggcga
     3661 agcgggcctg gccggcatcg ccgtcgaccc gagctggccg acgaagccct tcgtctacct
     3721 gcactacaac cagaacaccc cgcaccggct catcgttgcc cggtatcgcg ccaccgggaa
     3781 cctcaccgat ccgaccggcc tcacgatggc gctctcagac ccgtatgtca tcctcgacgt
     3841 cgatgacagc tccccgaacc acaatggttc gtgcctccgg ttcggcccga gcggcctcct
     3901 ctacgtgtcg ctgggcgacg acgtcttttc gtgcgttgcc caggattcca cgctgctgaa
     3961 aggggtcatc gcccgcatca ccgtggccaa ccttccgccg accggtccgg gccccgcgcc
     4021 ccgcagcctg atcctgccgc cgggcaaccc gttcgcgccc gacaccaccg acgcggaact
     4081 cgtctggtgt tacgggctgc gcaatccgta ccggttcacg gtcgatccgt acaccgaaga
     4141 gctgtacatc ggcgacgtgg ggggaacgga attcgacgag gcggatgttg ccgtgggcgg
     4201 cgaaaacttc ggctggccca tcctcgaagc gaacgactcg accggcgtct ggtgcttcca
     4261 ggggctgccg aaattcaccg atcccattct
//
LOCUS       DASVOD010000323        13076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_387144, whole genome shotgun sequence.
ACCESSION   DASVOD010000323 DASVOD010000000
VERSION     DASVOD010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13076
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387144"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF720_07270"
     CDS             <1..854
                     /locus_tag="VF720_07270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003218.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX7879194.1"
                     /translation="HPHGIELGILMRMLKDTTARNVQSFLVNEFSRVSPKSAEEILKG
                     ARIDVTASPSRVHRDSAEKLYQSINATKLMAPPTNILSPIGEDQLLAGMRNQFQADFY
                     TATSRGPQVYRGNPFLIEVGLAWNVKDMPGDELAELYRFANRVPLLYQQSACAVTKGV
                     ISTGWKSYGVQQSRGALPTGALVIMVHIASVWVPFTSESKEAIAHYPEIIKEIRLAIQ
                     ECGRRLGIFVRRGQREAMEAKKLSYIEKYIPHIGIALKEILKLSDKEEKDVVETLRGT
                     LERSRNL"
     gene            851..2056
                     /locus_tag="VF720_07275"
     CDS             851..2056
                     /locus_tag="VF720_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7879195.1"
                     /translation="MTTDRAEASLLGWRAEFPILDHTVYMVSHSLGAMPRGVYGELRA
                     FADAWASRGVRAWPEGWWEMPITVGNMIAGIVNAPAGSVAMVQNVTVGVEAIASSYDW
                     SAARNRIVTTEVDFPSVLYLLDGIARRGARVHRVPSPDGLYFDMQQLLEAIDEETALV
                     VVSQVLFKSAAIVDIEPIVARARQVGAHVLLDGYQSIGTVPCDVTKLDVDYLVGGSVK
                     WLCGGPGAGYVYAKPSLIPTLRPSFVGWQAHETPFTFDPSPITYRHDAGRFLNGTPHV
                     PCLYAAASGYRIIAEVGVHNIRARSLKLSQRLFDRADAAGFTINSPREANRRGATVTL
                     GVPEAQAVAKTLIAREFIVDYRPGAGIRVGPHFYNTEDEVDSIVAEVQNILKSGAWKE
                     HQAAVGEVQ"
     gene            2053..2673
                     /locus_tag="VF720_07280"
     CDS             2053..2673
                     /locus_tag="VF720_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029293791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX7879196.1"
                     /translation="MIYDITLPVDAKTAVWPGDHAYTIDWTMRKATGATVNVTKLTLS
                     PHTGTHADAFFHCSDEGPGMGEMSLDPYLGKARVLDAPGDGPIDVERLEKLNWKGATR
                     LLFKTRRSRVSDPYAIPFAHLLPAAVDELGKAGVILVGLDTPSMDEFHSKTIGAHHAL
                     MRHGIANLENLDLTEVPAGDYELIALPLKLMGVDGSPVRAVLRTVS"
     gene            2788..3015
                     /locus_tag="VF720_07285"
     CDS             2788..3015
                     /locus_tag="VF720_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEX7879197.1"
                     /translation="MTVFADPFSITKRDDEHSIGEWRWLELGRSSLGQLLVVVYSENT
                     RFKRLISARPASRKERRQYEEVSQAACPARR"
     gene            2975..3220
                     /locus_tag="VF720_07290"
     CDS             2975..3220
                     /locus_tag="VF720_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879198.1"
                     /translation="MKKSARRRAPRVGEMKPEYDFSNGVRGKHAAQFKDVVFVGLDPD
                     VAAVFNDSATVNRVLRGLIELISPAKPPSAPRRKRKS"
     gene            3217..4308
                     /locus_tag="VF720_07295"
     CDS             3217..4308
                     /locus_tag="VF720_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011972556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEX7879199.1"
                     /translation="MMSNKSVSKKIVTEAEQVHSKILKRDKPSMKFPIRSLANVRYTP
                     KQGYFEIRGLKKERTLTVNTVKTFAQTLRMMALSKQLVENDDIATKREAYYVSKNWGE
                     ARFLEQPESDTVMDDVEALFSVNREQLGFIPEEKGGEVAGQLVVIDRDRDSGRPLRID
                     CTKFGSGAYSIPISVEELQFETKAAFILVIETAGMFQRLVKHNYWKKANCILISMGGV
                     PTRACRRFIRRLADDKKLPVYVFVDGDPYGIANIYRTLKVGSGSAAHLNEFFCVPQAR
                     FLGITPQDIVDYELPTHPLKEIDVKRARDAIKNDPFFQHHKDWQKAMEKLLTMGVRAE
                     QQALAAHGLNYVIENYLPEKLRATDKFLP"
     gene            4335..4796
                     /locus_tag="VF720_07300"
     CDS             4335..4796
                     /locus_tag="VF720_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879200.1"
                     /translation="MFRKKMMTSFLMVTAGALLLAGWAPEASARRSGWGVGAIFRDDF
                     DFGMQARWNRLGGGRITLVPQLGYFFDDGAMDANFDGHLTLKKGSEEKPLNIYALGGV
                     NWVTDFDFGEVGFNVGGGIDKDFSAGLGGFAEIKYVISDADGMALTAGIHF"
     gene            complement(4826..5533)
                     /locus_tag="VF720_07305"
     CDS             complement(4826..5533)
                     /locus_tag="VF720_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7879201.1"
                     /translation="MFPGAILKLSILIPAYNEEATIEKVLDAVLAVPLPGTTGREIVV
                     VDDASTDRTPEILRRRASSEVRVFRQEKNQGKGSAIRRAIAEATGDICIIQDADLEYD
                     PRDFPLLLEPILEKGADAVYGSRFLGSHRVFLYWHFMANKSLTWLVNVLYDTMLTDME
                     TGYKAIRTPILREMRLRSNTFDIEPEITAKLFKKRRKVFEVPIRYNGRSYAEGKKIGL
                     KDAFIALGAIIRFRFGD"
     gene            complement(5599..7086)
                     /locus_tag="VF720_07310"
     CDS             complement(5599..7086)
                     /locus_tag="VF720_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX7879202.1"
                     /translation="MNVTQLPPILAKWAQKIEGVAASYGLDFYPVIFEMVDFETMNEV
                     AAYGGFPTRYRHWRWGMEYESLSKGYRYGLSKIYELVINNNPCYAYLLESNAVVDQKL
                     VMAHVYAHCDFFKNNISFSRTNRKMMDETANHAARVDRYIEEVGLERVETFLDACLTI
                     EDLVDPHAQFIQRRRTLRRDGEEEPAVVKKLRSKNYMDSYINPPEFLEAQQARLDEEA
                     RRMRSFPEEPERDILLFLMEHAPLEKWQRDILSIIREEAYYFAPQGQTKIMNEGWASF
                     WHSRMMTEKLLTVEELVDYADHHSGTVATSPGRLNPYKIGIELFRDIEERWNKGRFGK
                     EYDECDSWDRKRHWDTGLKQGREKVFEVRKIHNDVTFIDTFLTEEFCRQQKLFTFAMN
                     DKNQTWEIQSREFKKVREMLLFQLTNHGRPFIYVKNGNHANRGELLLWHRYEGIELKR
                     DYAEDTLRNLEMIWGRPVHVETVMAETDTLISCVGGEVTEAEITG"
     gene            complement(7083..8183)
                     /locus_tag="VF720_07315"
     CDS             complement(7083..8183)
                     /locus_tag="VF720_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX7879203.1"
                     /translation="MVLRVDQDHNRFREIIRGRIRKELKKYIAQGDMVGRQGKKLISI
                     PVPSVDLPHFRLGKNQGGVGQGDGNQGDPVGEGEESDVGQAGDQPGKHLLEVELTLDE
                     LAAILGEELELPRIEPRGTRSVVSQRDKYRSVRRTGPESLRHFKRTYKKALRRQIASG
                     VYNPDRPVIIPIKEDRVYRSWKDVEEPESSAVIVYMMDVSGSMGDEQKEIVRIEAFWI
                     DTWLQSQYRRLETRYIVHDAAAKVVDRHTFFHLRESGGTKISSAYDLARRVIEEEFSP
                     ADWNVYLFHFSDGDNWGGGDTETCLKLLGETLLPAANLFAYGQVESPYGSGQFIKDLE
                     QNLSGAENLVVSRIPSKDAIYGSIKEFLGKGR"
     gene            complement(8237..10285)
                     /locus_tag="VF720_07320"
     CDS             complement(8237..10285)
                     /locus_tag="VF720_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX7879204.1"
                     /translation="MTSRIDIVSIIREHQNLESYRELTWEGTFQDYVNIVAEDPRVAR
                     TAFQRVYDMILSYGTEEYTEYKKRIVHYKFFDDPFENGRDAVYGLDIPLMKLVNVFKS
                     AARRYGTERRVILLHGPVGSSKSTIARLLKKGLEAYSRTKEGALYSYSWIEKGPDGTE
                     SLHKCPMHEEPLHLIPPDIRPRLLAEINRSQNAETQVNVEGDLCPYCRRSFDDLYRKH
                     GGDWTEVIQNVKVQRIVLSEKDRVGIGTFQPKDEKNQDSTELTGDINYRKIAEYGTES
                     DPRAFNFDGEFNIANRGMIEFVEVLKLDVAFLYDLLGASQEHKIKPKKFAQTDIDEVI
                     LGHSNEPEYRKLQKNEYMEALRDRTLKIDVPYITKLDEEIKIYLKDYNPATIRGKHIA
                     PHSIEMASMWAILTRLEEPKKADLKLMQKLKLYNGRSLPGFTEDNVRELRKEVHREGL
                     SGISPRYVQDKISNALVSDVATSCVNPFMILNELESGLKHHSLISNDEERTRYRELLG
                     VVKDEYENVVKNEVQRAISADEEGITKLCANYIDNIKAYTQRERVRNKYTGQDEEPDE
                     RLMRSIEEKIDIPEGRKDDFRREIMNYIGALAIEGKTFDYKMNSRLQRALEGKLFEDQ
                     KDSIKLTSLVSSVVDKDTQERIDIVKSRLIKNYGYCDICATDVLHFVASIFARGDTKS
                     "
     gene            complement(10609..10953)
                     /locus_tag="VF720_07325"
     CDS             complement(10609..10953)
                     /locus_tag="VF720_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018936733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEX7879205.1"
                     /translation="MEECLFCRIAAGTIPARTVFEDDDVVAFEDIQPQAPVHLLLIPR
                     RHVANLNDLGSDDHHLVGRLLEVAKRLAGERGLAETGYRVVTNTGADAGQTVLHLHLH
                     LLGGRPLGWPPG"
     gene            11075..11503
                     /locus_tag="VF720_07330"
     CDS             11075..11503
                     /locus_tag="VF720_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879206.1"
                     /translation="MNRPRDNRWLAAIGAIVLVAFGCQQSPKNSAGDQVSKQEQDEIA
                     KILSDTAGGMNKMAVPAPTLPANIVFTRDDIPREGGYLQVTIGDVTDAESNQFLRRLK
                     SERCTCGCPHTIDQCLIEDPQCDVAHRLAAQVLREVQLGS"
     gene            complement(11488..11949)
                     /locus_tag="VF720_07335"
     CDS             complement(11488..11949)
                     /locus_tag="VF720_07335"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010496151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7879207.1"
                     /translation="MDDFSRYEMPIRVRYAETDQMGQVYHANYLVWFEAGRGHVMREL
                     GLPYGRLEEAGFLLPVTRFDALMRIPAVYDDELRVRTWVELARSRAVHFRYHVVRGHE
                     ILAEGSTLHVSMDRKGRAVRMPVAATELLTAYAARWPAPPARRPKPGHDPS"
     gene            12008..>13076
                     /locus_tag="VF720_07340"
     CDS             12008..>13076
                     /locus_tag="VF720_07340"
                     /inference="COORDINATES: protein motif:HMM:NF019585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX7879208.1"
                     /translation="MPTPETTATRRLYHEDAGIIEFEATLLETRPAPGDLAGQADWRL
                     DRTAFYATSGGQPHDTGWLGVAPVMDVFDEDGLIWHRVANPDGAAPATGATLAGTIDK
                     ARRRDHTQQHTGQHILSQAFIETCGAETVSFHLGAESVTIDLACERLAPEAILAAERR
                     ANEVVFENRPIVLHWTTREEINRFPLRKPPVVEGPIRIVEVEGYDWSACGGTHVGRTG
                     EIGPIRILGTTSVKQGVRVDFRCGWRALDDYLWKHALVRDVAARFSTLDRQLGAAIER
                     LEDDNQKMRKRLKQFEEERLDRDALSLLATVGTDGARRFLQVERPGADIEELKGLAQR
                     LRGTAGVVALLAGRTDDGSAPL"
BASE COUNT         2257 a         4176 c         4228 g         2415 t
ORIGIN      
        1 cccacccgca cggcatcgag ctgggcatcc tgatgcggat gctgaaggac acgaccgcac
       61 gcaacgtgca gtcgttcctg gtcaacgagt tcagccgcgt gagccccaag tcggccgagg
      121 aaatcctgaa gggggccagg atcgacgtca ccgcctcccc ctcgcgcgtc catcgcgatt
      181 cggccgagaa gctctaccag tcgatcaatg ccacgaagct gatggcgccg cccaccaaca
      241 tcctctcgcc gatcggcgag gaccagctcc tggccggcat gcgaaaccag ttccaggccg
      301 acttctacac ggccaccagc cgtggaccgc aggtataccg cggcaacccg ttcctcatcg
      361 aagtggggct ggcctggaac gtcaaggaca tgccgggcga tgaactcgcc gagctctacc
      421 gcttcgccaa ccgtgtgccg ctcctctacc agcagtcggc ctgcgcggtc accaagggcg
      481 tgatctcgac ggggtggaag tcgtacggcg tgcagcagag ccgcggggcg ctcccgacgg
      541 gggcgctcgt gatcatggtg cacatcgcct cggtctgggt gccgttcacc tccgagagca
      601 aggaagccat cgcccactat ccggagatca tcaaggagat ccggctggcc atccaggaat
      661 gcggccgccg gctcggcatc ttcgtccgac gcggccagcg cgaggccatg gaggccaaga
      721 agctctcgta catcgagaag tacatcccgc acatcggcat cgcgctcaag gaaatcctca
      781 agctctccga caaggaagag aaggacgtcg ttgagacgtt gcggggcacg ctcgagcgga
      841 gccgcaacct gtgacgaccg acagggccga ggcctcgctc ctcggctggc gtgccgaatt
      901 cccgatcctc gaccacaccg tgtacatggt gagccacagc ctgggggcca tgccgcgcgg
      961 cgtgtacggc gagttgcgcg ccttcgccga cgcctgggcc tcgcgcggcg tccgcgcctg
     1021 gcccgagggg tggtgggaga tgccgatcac ggtggggaac atgatcgcgg gcatcgtgaa
     1081 cgctccggcc ggcagcgtgg ccatggtgca gaacgtcacg gtgggggtgg aagccatcgc
     1141 gtccagctac gactggagcg ccgcgcgcaa ccggatcgtc acgaccgagg tcgacttccc
     1201 gtccgtgctc tacctccttg acggcatcgc ccgccgcggc gcccgcgtgc accgcgtgcc
     1261 gagccccgac ggcctctact tcgacatgca gcagctcctc gaggccatcg acgaagaaac
     1321 ggcgctggtc gtcgtgagcc aggtgctctt caagagcgcc gccatcgtcg acatcgagcc
     1381 gatcgtggcc cgcgcccgcc aggtgggggc gcatgtcctg ctcgacggct accagtcgat
     1441 cggcaccgtt ccctgcgatg tcacgaaact ggatgtcgac tacctggtcg gcggttcggt
     1501 gaagtggctg tgcggcggcc cgggcgccgg ctacgtgtac gccaagccgt cgctgatccc
     1561 aacgctccgg ccatcgttcg tcggctggca ggcccacgag acgccgttca cgttcgaccc
     1621 atcgcccatc acctatcgcc acgatgccgg ccgcttcctg aacggcacgc cgcatgtgcc
     1681 ctgcctctac gccgccgcca gcggctaccg gatcatcgcc gaagtggggg tacacaacat
     1741 ccgcgcccgt tcgctgaaac tctcccagcg cctgttcgac cgcgccgatg ccgcgggctt
     1801 caccatcaac agcccgcgcg aagccaatcg gcgcggcgcc accgtcacgc tcggcgttcc
     1861 cgaagcccag gccgtggcca agacgctcat tgcccgcgag ttcatcgtcg actaccgccc
     1921 cggcgccggc atccgcgtcg gcccccactt ctacaacacc gaggacgaag tcgactccat
     1981 cgtggccgag gtccagaaca tcctgaagtc cggcgcctgg aaggaacacc aggccgccgt
     2041 gggcgaggtg cagtgatcta cgacatcacg ctcccggtcg atgccaaaac ggcagtgtgg
     2101 ccgggcgacc acgcgtacac gatcgactgg acgatgcgga aggccaccgg cgccaccgtc
     2161 aacgtgacca agctgacgtt gagcccccac accggcaccc acgccgacgc cttcttccac
     2221 tgctcggacg agggaccggg aatgggggag atgtccctcg acccctacct cggcaaggcg
     2281 cgcgtcctcg atgcccccgg cgacggtccc atcgatgtcg aaagactgga aaaactcaat
     2341 tggaaaggcg cgacgcgcct tctcttcaaa acgcggcgga gccgcgtttc cgatccatac
     2401 gcaatcccgt tcgcgcacct cctgccggcg gcagttgacg aattgggcaa ggctggggtc
     2461 attctcgtgg gactcgacac gcccagcatg gatgaatttc attcgaagac aatcggcgcc
     2521 caccatgcgt tgatgcgcca tggcatcgcg aatctcgaga atctcgacct gacggaagtg
     2581 ccggcggggg attacgagct gatcgcgctt cccctcaagc tcatgggcgt ggatgggagt
     2641 ccggtgcgag ctgtattgag aacagtatcc tgagtattcc cccgattctt cccccgaaag
     2701 atcggctgac atgcagcccg acttccgatg gaactcggca aaggctgtag cgaactggcg
     2761 aaagcacgga gtatcgttcg gggaggcatg acagtgttcg ccgatccgtt cagtatcacc
     2821 aagcgggatg acgaacactc aatcggcgaa tggagatggc tggagttggg acgatcgagt
     2881 ctcggtcagc tactggttgt cgtctacagc gagaacactc gtttcaagcg actgatcagc
     2941 gcgcgacccg cgtccagaaa ggagcgtcgt cagtatgaag aagtcagcca ggcggcgtgc
     3001 cccgcgcgtc ggtgaaatga agccggaata cgatttttca aatggagttc gcggcaagca
     3061 cgccgctcag ttcaaggacg tcgtcttcgt cggacttgac ccggatgtcg cggcagtctt
     3121 caacgattca gcgactgtca atcgagtgct tcgtggcctg atcgaactca tttcaccggc
     3181 aaagccgccc tcagcgcctc gccgtaaaag gaaatcctga tgtccaacaa atccgtctcc
     3241 aagaagatcg tcaccgaggc cgaacaggtc cacagcaaga tcctgaagcg cgacaagccg
     3301 agcatgaagt tcccgatccg cagcctggcc aacgtgcgct ataccccaaa acagggctat
     3361 ttcgagatcc gcggcctcaa gaaggaacgt acgctcaccg tgaacacggt gaagacgttc
     3421 gcgcagacgc tccggatgat ggcgctctcc aagcagctcg tggagaacga cgacatcgcc
     3481 acgaagcgag aggcctacta cgtctccaag aactggggcg aggcccgatt cctggagcag
     3541 ccggagtcgg acacggtgat ggacgatgtc gaggccttgt tcagcgtcaa ccgcgagcag
     3601 ctcggtttca tcccggagga aaaggggggc gaagtggcgg ggcagctggt cgtgatcgat
     3661 cgtgatcgcg actcggggcg gccgcttcgg atcgactgca ccaagttcgg cagcggcgcc
     3721 tactcgatcc cgatctcggt tgaggagctg cagttcgaga cgaaggccgc attcattctc
     3781 gtcatcgaga cggccggcat gttccagcgg ctggtcaagc acaactactg gaagaaggcc
     3841 aactgcattc tgatctcgat gggaggcgtg ccgacgcgcg cctgccgccg cttcatccgg
     3901 cgcctggccg acgacaagaa gctgccggtg tacgtgttcg tcgatggcga tccgtacggc
     3961 atcgccaaca tctatcgcac gctcaaggtg ggctcgggaa gcgcggcgca cctgaacgag
     4021 ttcttctgcg taccgcaggc ccggttcctc gggatcacgc cgcaggacat cgtcgattac
     4081 gaactcccca cgcacccgct caaggaaatc gacgtcaagc gggcccgcga tgccatcaag
     4141 aacgatccct tcttccagca ccacaaggac tggcagaagg ccatggaaaa gctcctcacg
     4201 atgggcgtcc gtgccgagca gcaggcgctg gccgcgcacg gcctcaacta cgtcatcgag
     4261 aactaccttc cggagaagct ccgggccacc gacaaattcc tgccataggt tcgactcttg
     4321 aggaggggag aaacatgttc cgaaagaaga tgatgacatc gttcctgatg gtcacggccg
     4381 gtgccctgct cctcgcgggg tgggcgcccg aggcgtccgc gcggcgcagc gggtggggag
     4441 tgggcgccat cttccgcgat gatttcgatt tcggcatgca ggcgcgctgg aaccggctgg
     4501 ggggcgggcg cattaccctc gtgccgcagc tcggctattt cttcgatgac ggcgcgatgg
     4561 acgccaattt cgacggccac ctcacgctca agaagggaag cgaggagaaa cccctcaaca
     4621 tctacgcgct cggcggcgtg aactgggtca ccgacttcga tttcggggaa gtcggtttca
     4681 acgtcggcgg cggcatcgac aaggatttct ccgccggact gggtgggttt gcggagatca
     4741 agtacgtgat ctccgacgca gacggcatgg cgctgacggc cggcatccac ttctagtcgg
     4801 gcgccagtcg cccgacagtc gaacgctagt ccccgaaccg gaaccggata atggcgccga
     4861 gggcgatgaa ggcgtccttc aggccgatct tcttcccctc ggcgtaggac cgtccgttgt
     4921 agcggatcgg gacctcgaac accttgcgcc gcttcttgaa cagcttggcc gtgatctccg
     4981 gctcgatgtc gaacgtgttc gaccggagac gcatctcgcg caggatcggc gtgcgtatcg
     5041 ccttgtagcc cgtttccatg tcggtgagca tcgtgtcgta cagcacattc accagccagg
     5101 tgagcgactt gttggccatg aagtgccagt acaggaacac gcgatggctc cccaggaagc
     5161 ggctcccgta caccgcgtcc gcccctttct cgaggatcgg ctcgaggagc agggggaagt
     5221 cccgggggtc gtattccagg tcggcgtcct ggatgatgca gatgtcgccc gtggcttcgg
     5281 cgatggcgcg ccggatggcc gaacctttcc cctggttctt ttcctgccgg aagacgcgga
     5341 cctcgctcga cgcgcgccgc cgcaggattt cgggcgtccg gtcggtcgag gcatcgtcca
     5401 cgacgacgat ctcccgcccc gtcgtgccgg gaagcgggac cgcgagcacc gcgtcgagaa
     5461 ccttctcgat cgtggcttcc tcgttgtagg cgggaatgag gatggaaagc ttcaggatcg
     5521 cccccggaaa gatggttgca acaggggcgg aactctatgg ggcgatgagg gatgagtcaa
     5581 ccggtgggga cggccgcgtc accccgtgat ctccgcctcc gtcacctcgc cccccacgca
     5641 cgagatcagc gtgtcggtct cggccatgac cgtttccacg tggaccggcc ggccccagat
     5701 catctcgagg ttcctgagcg tgtcctcggc gtagtcgcgc ttcagctcga taccctcgta
     5761 acggtgccag aggaggagct cgccgcggtt ggcgtggttg ccgttcttca cgtagatgaa
     5821 cgggcggccg tggttggtga gctggaagag gagcatctcg cgcaccttct tgaattcgcg
     5881 gctctggatc tcccaggtct ggttcttgtc gttcatggcg aaggtgaaga gcttctgctg
     5941 gcggcagaat tcctcggtga gaaaggtgtc gatgaacgtg acgtcgttgt ggatcttgcg
     6001 cacctcgaac accttctcgc gcccctgctt caggccggtg tcccaatggc gcttccggtc
     6061 ccaggagtcg cattcgtcgt actccttgcc gaagcgcccc ttgttccagc gctcctcgat
     6121 gtcgcggaag agctcgatgc cgatcttgta ggggttcagc cgtccggggc tggtggccac
     6181 cgttcccgag tggtggtcgg catagtcgac cagctcctcc accgtgagga gcttctcggt
     6241 catcatgcgc gagtgccaga agctggccca cccctcgttc atgatcttgg tctgtccctg
     6301 cggggcgaaa tagtaggcct cctcgcggat gatcgagagg atgtcgcgct gccacttttc
     6361 gagcggtgcg tgctccatga ggaagaggag gatgtcgcgc tcgggctcct cgggaaacga
     6421 gcgcatccga cgggcttcct cgtccagtcg ggcctgctgg gcctcgagga actccggtgg
     6481 attgatgtag gagtccatgt agttcttcga gcggagcttt ttcacgaccg ccggctcctc
     6541 ttcaccgtcg cggcggaggg tgcggcgccg ttggatgaac tgggcatgcg gatcgaccag
     6601 gtcttcgatg gtgaggcatg cgtcgaggaa cgtttccact cgttcgagcc cgacctcctc
     6661 gatgtagcgg tcgacccggg cggcgtgatt ggccgtctcg tccatcatct tgcggttggt
     6721 gcggctgaag ctgatgttgt tcttgaagaa gtcgcagtgc gcgtagacgt gcgccatgac
     6781 cagcttctgg tcgacgaccg cgttcgattc gagcaggtag gcgtagcagg ggttgttgtt
     6841 gatcacgagc tcgtagatct tcgaaagccc gtagcgatat cccttggaga ggctttcgta
     6901 ctccatcccc cagcgccagt gccggtagcg ggtggggaag ccgccgtagg cggccacctc
     6961 gttcatcgtc tcgaagtcca ccatctcgaa gatgacgggg tagaagtcga ggccgtacga
     7021 ggcggccacg ccctcgatct tctgggccca cttggccagg atcggcggaa gctgggtgac
     7081 gttcatcgcc ccttccccag gaattccttg atcgatccgt agatggcgtc cttgctcggg
     7141 atgcggctca ccaccaggtt ctccgccccg gacaggttct gctccaggtc cttgatgaac
     7201 tggcccgagc cgtaggggct ttccacctgg ccataggcga acaggttggc ggccgggagg
     7261 agcgtttcgc cgaggagctt gaggcaggtt tcggtgtcgc cgccgcccca gttgtcgccg
     7321 tccgagaagt ggaagaggta cacgttccag tcggccggcg agaactcctc ctcgatgacc
     7381 cggcgggcca ggtcgtaggc cgaggaaatc ttggtgccgc ccgactcgcg caggtggaag
     7441 aaggtgtgcc ggtcgacgac cttggcagcc gcgtcgtgca cgatgtagcg cgtctcgagg
     7501 cgccggtact ggctctggag ccaggtgtcg atccagaacg cctcgatccg gacgatctcc
     7561 ttctgctcgt cgcccatcga gcccgacacg tccatcatgt agacgatgac cgcgctcgat
     7621 tccggctcct ccacatcctt ccaggagcgg tagacgcggt cttccttgat cgggatgatg
     7681 accggccggt cgggattgta gacaccgctg gcgatctggc gccgcagcgc cttcttgtag
     7741 gtgcgcttga agtgccggag gctctcgggg ccggtgcgcc ggacgctccg gtacttgtcc
     7801 cgctgggaga cgacgctgcg ggtgccgcgc ggttcgatgc gggggagctc gagctcctcg
     7861 ccgaggatgg ccgccagctc gtcgagcgtc agctccactt ccagtaggtg cttgccaggc
     7921 tggtcgccgg cctgcccgac gtcgctctct tcgccttcgc cgaccggatc cccctggttg
     7981 ccgtcaccct gtcccacgcc cccctggttc tttcccaggc ggaagtgcgg caggtcgacg
     8041 ctgggcaccg ggatggagat gagcttcttc ccctggcgac cgaccatgtc gccctgggcg
     8101 atgtatttct tcagctcttt ccggatgcga ccgcgaatga tctcgcggaa acgattgtga
     8161 tcctggtcga cacgaaggac cattcaacga ctccgacggt tcggcccttc ggccgtgggc
     8221 cctctatgcg gaaggtttac gacttggtgt cgccgcgggc gaagatcgag gccacgaagt
     8281 gcaggacgtc cgtggcgcag atgtcgcagt agccgtagtt cttgatgagc cggctcttca
     8341 cgatgtcgat ccgctcctgg gtgtccttgt cgaccacgct cgagacgagg ctggtgagct
     8401 tgatcgagtc cttctggtcc tcgaagagct tcccttccag ggcgcgttgc agccttgagt
     8461 tcatcttgta gtcgaacgtc ttcccctcga tggccagggc gccgatgtag ttcatgatct
     8521 cgcgccggaa gtcgtccttg cgcccctcgg ggatgtcgat cttctcctcg atggagcgca
     8581 tcagccgctc gtcgggctcc tcgtcctggc cggtgtactt gttgcggacc cgctcgcgct
     8641 gcgtgtaggc cttgatgttg tcgatgtagt tggcgcagag cttggtgatc ccttcctcgt
     8701 cggccgagat ggcccgctgg acctcgttct tgaccacgtt ctcgtactcg tctttgacca
     8761 cgccgagcag ctcgcggtaa cgggtgcgct cctcgtcgtt ggagatgagc gagtggtgct
     8821 tcagtcccga ctcgagctcg ttcaggatca tgaacgggtt gacgcaggag gtggccacgt
     8881 cgctcaccag ggcattcgag atcttgtcct gcacgtagcg gggcgaaatg ccggagagtc
     8941 cctcgcgatg cacctccttg cgcagctcac ggacgttgtc ttccgtgaag cccggcaggc
     9001 tccggccgtt gtagagcttg agcttctgca tgagcttgag atcggccttc ttcggctcct
     9061 cgaggcgggt caggatggcc cacatcgagg ccatctcgat cgagtgcggc gcgatgtgct
     9121 tgccgcggat cgtggccggg ttgtagtcct tgaggtagat cttgatctcc tcatcgagct
     9181 tggtgatgta cggcacgtcg atcttgagcg tgcggtcccg caaggcctcc atgtactcgt
     9241 tcttctggag cttgcggtac tcgggctcgt tggagtggcc gaggatcacc tcgtcgatgt
     9301 cggtctgggc gaacttcttc ggcttgatct tgtgctcctg gctggcgccg aggaggtcgt
     9361 agaggaaggc cacgtcgagc ttcagcactt ccacgaactc gatcatgccg cggttggcga
     9421 tattgaactc gccgtcgaag ttgaaagccc tgggatcgct ctcggtgccg tactcggcga
     9481 tcttgcggta gttgatgtcg ccggtgagct cggtagagtc ctggttcttc tcgtccttgg
     9541 gctggaaggt gccgatgccc acgcggtcct tctcggacag gacgatgcgc tgcaccttca
     9601 cgttctggat gacttccgtc cagtcgccgc catgcttgcg gtagaggtcg tcgaagctcc
     9661 ggcggcagta ggggcagagg tctccttcca cgttcacctg ggtttcggcg ttctggctcc
     9721 ggttgatctc ggcgaggagc ctcgggcgga tgtccggcgg gatcaggtgg agcggctcct
     9781 cgtgcatcgg gcacttgtgg aggctttcgg tgccgtccgg ccccttctcg atccagctgt
     9841 acgagtagag ggcgccttcc ttggtgcgcg aataggcctc gagccccttc ttgaggagcc
     9901 gggcgatggt gctcttggag gagccgaccg ggccgtgcag caggatgacg cggcgctcgg
     9961 tgccgtagcg gcgcgccgcc gacttgaaga cgttgacgag cttcatcagc gggatgtcga
    10021 ggccgtacac cgcatcccgg ccgttctcga acggatcgtc gaagaacttg tagtgaacga
    10081 tccgcttctt gtactcggtg tattcctcgg tgccgtacga caggatcatg tcgtacaccc
    10141 gctggaaggc ggtccgcgcc acgcgcggat cttcggcgac gatgttgacg tagtcctgga
    10201 aggtcccttc ccaggtgagt tctcgatagc tctccagatt ctggtgttcg cgaatgatgg
    10261 agacgatatc gatacgcgag gtcatgtcct catccccctc gactgaatgt cccggacggc
    10321 ggtgatcgcc gggcgggcaa tggcagcgtt aaccggaccc ctgaattcaa gatagggaag
    10381 accggcgcac ttccgcaaag ttccttttct gcaagactcg ggccgattcg ggcccggggg
    10441 cgcaccccgg tgggatcggg gcggtatgtc ggcgggtgac aagggcttcg gtcgggagaa
    10501 atcgacatcc ttccgggtac agcaccggcc ggcccggatg cggcgtggcc gcccggacgg
    10561 gcgaagttcg accggtgccg tccgatttcg gaccggaccg gaatcaggtc atcccggagg
    10621 ccaaccaagg ggccgccccc ccagaagatg aaggtgcagg tggagcacgg tctgtccggc
    10681 atccgcacca gtattcgtga ccacgcggta tccggtttcg gcaaggcccc gttcgccggc
    10741 cagccgtttg gccacctcga gaaggcggcc caccaggtgg tggtcgtcgg aacccaggtc
    10801 attcaggttg gccacgtggc gccgcgggat caggagtaga tggaccggtg cctgcggctg
    10861 gatgtcctcg aaggcgacga cgtcgtcgtc ctcgaaaacg gtccgcgccg gtatggtccc
    10921 ggccgcaatc ctgcagaaga ggcattcctc catgcaatca acgtagcacc ttcgcggcgc
    10981 tgcgcgccaa cccattccgc accggagtca tctggtatca tcggtgaccc tcttggcggg
    11041 cacctcaaac catcatggaa ggaacaattc ggagatgaat cgaccccgtg acaatcgctg
    11101 gctggccgcc atcggcgcca tcgtcctggt ggccttcggg tgccagcaga gtccgaaaaa
    11161 cagtgcgggc gaccaggtca gcaagcagga gcaggacgaa atcgccaaga tcctttccga
    11221 taccgccggc ggcatgaaca agatggccgt cccggcgccc acgctcccgg ccaacattgt
    11281 cttcacccgc gacgacatcc cgcgcgaggg tggctacctc caggtgacga tcggcgacgt
    11341 gaccgatgcc gagtcgaacc agttcctccg ccggctgaag tccgagcggt gcacctgcgg
    11401 ctgcccgcac accatcgacc agtgcctgat cgaggacccg cagtgcgacg tggcccaccg
    11461 gctggccgcg caggtcctgc gcgaggttca gctcgggtcg tgacccggtt tcggccggcg
    11521 cgcgggcggc gccggccatc gggcggcgta cgccgtgagg agctcggtcg cggccacggg
    11581 catccggacg gcccgtccct tacggtccat ggacacgtgg agcgtggacc cttccgcgag
    11641 gatctcgtgg ccacgcacga cgtggtatcg gaagtgcacg gcgcggctcc gggccagttc
    11701 cacccaggtg cgtacccgca gttcgtcatc atagacggcc ggtatccgca tcagggcatc
    11761 gaaacgggtg accgggagca ggaagcccgc ttcttccagg cggccgtagg ggagccccag
    11821 ctcccgcatc acgtgtcccc gcccggcctc gaaccagacc aggtagttcg catgatagac
    11881 ctgccccatc tggtcggttt ccgcgtagcg gacccggatc ggcatttcgt accgggaaaa
    11941 atcgtccatg gcccctcttc cgagcgccct tgggagggcc cgcgcccgct cctataatgg
    12001 tgcgaccgtg ccaacccctg aaaccaccgc cacccgccgc ctctaccacg aggacgccgg
    12061 catcatcgaa ttcgaggcga cgctgctgga aacccgaccc gcaccgggag acctcgccgg
    12121 ccaggccgac tggcgcctcg atcggacggc attctacgcc acctcgggcg gccagccgca
    12181 cgatacgggg tggttgggag tcgccccggt catggacgtg ttcgacgagg atggcctcat
    12241 ctggcaccgg gtggccaacc cggatggtgc agcgcccgcc acgggagcca cgctggccgg
    12301 tacgatcgac aaggcccgcc ggcgcgacca tacgcagcag cacaccggcc agcacatcct
    12361 ttcccaggcg ttcatcgaga cctgtggcgc cgagacggtg tcgttccacc ttggggccga
    12421 gtcggtcacc atcgacctcg catgcgagcg cctggctccg gaggccatcc tggccgccga
    12481 gcgacgggcc aacgaggtcg tcttcgagaa tcggccgatc gtcctgcact ggacgacgcg
    12541 cgaagaaatc aaccgcttcc cgctccgcaa gccgccggtg gtggaggggc cgatccgcat
    12601 cgtcgaagtg gaaggttacg actggtcggc ctgcggcggc acgcacgtgg ggcgcaccgg
    12661 ggaaatcggg ccgatccgga tcctcggcac caccagcgtg aagcagggtg tgcgcgtcga
    12721 cttccgttgc ggctggcgcg cgctcgacga ctacctctgg aagcatgcgc tcgtgcgcga
    12781 cgtggccgca cgcttctcca cgctcgaccg gcagctcggc gcggccatcg agcgcctgga
    12841 agacgacaac cagaagatgc gaaagcgcct caagcagttc gaggaggagc ggctggatcg
    12901 cgatgcgctg tcgctgctgg ccacggtcgg gaccgacggc gcgcgccgct tcctccaggt
    12961 ggagcggccg ggcgccgaca tcgaggaatt gaagggactg gcccagcggc ttcgcggcac
    13021 ggcgggtgtc gtggcgctgc tggcgggacg taccgacgat ggcagcgcgc cgctcg
//
LOCUS       DASVOD010000324         3085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_805119, whole genome shotgun sequence.
ACCESSION   DASVOD010000324 DASVOD010000000
VERSION     DASVOD010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805119"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1224)
                     /locus_tag="VF720_07345"
     CDS             complement(16..1224)
                     /locus_tag="VF720_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX7879209.1"
                     /translation="MSTTATHLASAGGTPVRSAFLPYALPQFGPEEEQEVIQALASGW
                     ITTGPRTKKLEEQLAAYVGSKQAICVDSCTAALHLGLAALNLKPGDEVITTPITFCAT
                     ANTILHAGGKVVLADVEADTLNLSVAAVEKAITPKTKAIVPVHYAGHPCEMDDLMALA
                     KKHNLAVIEDAAHAIGAEYRGTKIGTIGQATCFSFYATKNMTTAEGGAITLNDEELAA
                     NIRILSLHGMSKDAWKRYTSAGSWYYEVVAPGFKYNMTDIEAALGLHQLAKVDAFNAT
                     RRRYATIYDKAIDAMGGGVHHLTPRAHVLSSVHLYPILVELEALTIDRAQFIEELKAE
                     NIGTTVNFIPIHYHPYYRDNLPYKKGDFPVAERAYERLISLPLYPRMTEDDLGLVMEA
                     VTKIARAYRR"
     gene            complement(1287..2279)
                     /locus_tag="VF720_07350"
     CDS             complement(1287..2279)
                     /locus_tag="VF720_07350"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7879210.1"
                     /translation="MNEPKLIMVTGGAGYIGSHLVRQLLDKGYAVRNLDKLAFGDHAI
                     KELSGHPNYSHINGDIRHIEDVVAAAKGAYGVIHLAGIVGDPACELDHDATWSINLEA
                     TKLMVECCKYAGIQRFVFASTCSVYGAADDYTLNEGSVLNPVSLYAETNLASERIILK
                     GFEGTDVVPTIVRFATVYGASRRMRFDLVVNIMTAKAVKDGAVKVFGGDQWRPNVHCQ
                     DAAAGLAACFEAPADKVKNEIFNIGSDEQNYKIQELGELVARCVPGTKVETMGESPDR
                     RSYKVSFEKARQVLGFTPKFRVEDGVAEIQRMLENGNVENYQADRYYNVRYIYK"
     gene            complement(2518..>3085)
                     /locus_tag="VF720_07355"
     CDS             complement(2518..>3085)
                     /locus_tag="VF720_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HEX7879211.1"
                     /translation="LKLVGTESNRAAIGARITWKAQGKRYVQQITGGTGYLSQNDPRV
                     LFPLGTTVGPDTFDIRWPSGAFQRVVALARNQTHTIIEADQTAVPVVDGAPPAVLALA
                     QNSPNPFQNDTAIRFALPAAGRVTLEILDVQGRLVRKILDQELANGVWEERWDGRTGD
                     GAPAASGVYFYRLTTPEGSRTRRLILAR"
BASE COUNT          454 a         1007 c         1034 g          590 t
ORIGIN      
        1 tcgatccgtc cccggtcagc ggcggtacgc ccgggcgatc ttggtcaccg cttccatcac
       61 caggccgagg tcgtcctcgg tcatccgggg atagagcggc agcgagatca gccgttcgta
      121 ggcgcgctcg gccacgggga aatcaccctt cttgtacggc aggttgtcgc ggtagtacgg
      181 gtggtagtgg atcggaatga agttgacggt ggtgccgatg ttctcggcct tgagctcctc
      241 gatgaactgc gcccggtcga tggtgagcgc ctcgagttcg acgaggatcg ggtagaggtg
      301 caccgacgac agcacgtgcg cgcgcggcgt gaggtgatgc acgccgccgc ccatcgcgtc
      361 gatggccttg tcgtagatcg tggcgtagcg cctccgcgtg gcgttgaacg catccacctt
      421 ggccagctgg tgcaggccga gtgccgcctc gatgtccgtc atgttgtact tgaagcccgg
      481 cgccacgacc tcgtagtacc acgaaccggc ggaggtgtag cgcttccagg cgtccttgct
      541 catcccgtgc agcgacagga tgcggatgtt ggccgccagc tcctcgtcgt tcagggtgat
      601 ggcgccccct tcggccgtcg tcatgttctt ggtggcgtag aacgagaagc aggtagcctg
      661 cccgatggtg ccgatcttcg ttcccctgta ctcggcgccg atggcgtggg ccgcatcctc
      721 gatgaccgcc aggttgtgct tcttcgccag cgccatcagg tcgtccatct cgcaggggtg
      781 ccccgcgtag tgcaccggga cgatggcctt cgtcttcggc gtgatggcct tctccaccgc
      841 ggccaccgac aggttcagcg tgtccgcctc gacgtcggct aggaccacct tgccgccggc
      901 atgaaggatc gtgttggccg tggcgcagaa cgtgatgggc gtggtgatga cctcatcgcc
      961 cggcttcagg ttcaaggcgg ccaggcccag gtgcagcgcc gccgtgcacg aatcaacgca
     1021 gatggcctgc ttcgagccga cgtaggccgc cagctgctct tccagcttct tggtgcgcgg
     1081 gcccgtcgtg atccagcccg acgccagagc ctggatgact tcctgctctt cctccgggcc
     1141 gaactggggc agggcgtagg gcaggaatgc tgagcggacg ggtgtgccgc cggcggatgc
     1201 caggtgtgtg gcggtcgtac tcaagggggc gcctccagat gtcgcagaac aatagcggtc
     1261 accagtcgtt ccgactggcg acgacattac ttgtaaatgt accggacgtt gtaataccga
     1321 tccgcctggt agttctccac gttgccgttc tccagcatcc gctgaatctc ggccacgcca
     1381 tcctccacgc ggaacttcgg cgtgaagccc agcacctggc gggccttttc gaacgacacc
     1441 ttgtaggagc ggcggtcggg gctctccccc atcgtctcga ccttcgtacc ggggacgcaa
     1501 cgcgccacca gctcgccgag ctcctggatc ttgtagttct gctcgtcgga cccgatgttg
     1561 aagatctcgt tcttgacctt gtcggccggc gcctcgaagc aggccgccag gcccgccgcg
     1621 gcgtcctggc agtgcacgtt ggggcgccac tggtcgccgc cgaacacctt cacggcgccg
     1681 tccttcaccg ccttggccgt catgatgttg accaccaggt cgaagcgcat ccgccggctc
     1741 gcgccgtaca cggtggcaaa gcggacgatc gtgggcacca cgtccgtccc ctcgaatccc
     1801 ttcaggatga tccgctcgga agccaggttc gtttccgcat agaggctgac cgggttcagc
     1861 accgatccct cgttcagcgt gtagtcatcc gccgcgccgt acaccgagca ggtgctggcg
     1921 aagacgaagc gctggatgcc cgcgtacttg cagcactcca ccatcagctt ggtggcctcg
     1981 aggttgatcg accaggtggc gtcatgatcg agctcgcagg ccgggtcgcc cacgatgccg
     2041 gccaggtgga tgacgccgta ggcccccttg gccgccgcca ccacgtcctc gatgtgccgg
     2101 atgtcgccgt tgatgtggct gtagttcggg tgccccgaga gttccttgat ggcgtgatcg
     2161 ccgaaggcca gcttgtcgag gttgcggacg gcatacccct tgtcgaggag ctgccggacc
     2221 aggtggctgc cgatgtagcc ggccccgccg gtcaccatga tcagtttcgg ttcgttcatc
     2281 gggagaagac ggcgcctttc gctcaggggg acccggccgc gcgcacgaac gggtgcggcg
     2341 taaaccttgg aattgtatca actttggcga tgcgggctcc agtgttgcct gaggggcttt
     2401 ccgagccgga ttggaccggt cggaacggcc ccccggcggc cgtaagccgt tcgccagggc
     2461 gacgatcggg ttatcggccg tttccgctcc gggattagcg gctgggttgg gtgcccctca
     2521 cctcgccagg atcagccgcc tggtccgcga gccttcgggc gtcgtcaatc ggtagaaata
     2581 gacgcccgag gccgccggcg ccccatcccc cgtccgcccg tcccaccgct cctcccacac
     2641 accgttggcc agctcctggt caaggatctt gcggaccaga cgcccctgga cgtcgaggat
     2701 ctcgagcgtg acgcgtcccg ctgccggcag ggcaaagcgg atggccgtgt cgttctggaa
     2761 cgggttgggc gagttctgcg ccagggccag gaccgccggc ggggcgccat ccaccaccgg
     2821 gacggccgtc tggtcggcct cgatgatcgt gtgtgtctgg tttcgcgcga gggccacgac
     2881 gcgctggaag gcgcccgacg gccagcggat gtcgaaggta tccgggccga cggtggtgcc
     2941 gagcgggaag aggacccgcg gatcgttctg gctgaggtag ccggtgccgc cggtgatttg
     3001 ctgcacgtag cgcttcccct gcgccttcca ggtgatgcgc gcgccgatgg cggcgcggtt
     3061 gctctcggtg ccgaccagct tcagt
//
LOCUS       DASVOD010000325         5513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_284155, whole genome shotgun sequence.
ACCESSION   DASVOD010000325 DASVOD010000000
VERSION     DASVOD010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284155"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VF720_07360"
     CDS             <1..546
                     /locus_tag="VF720_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879212.1"
                     /translation="VPGSGAIDWGIGLYPPGHAFTSKSGVHPGGIAFDAPVGATESMN
                     VTISQPGLYTVVVWKNRPADLGNTGTMTLLFDRQTTGVPDPGRPLGNRLIQGVRPNPV
                     IGPATVEVELTAASNVVLSIFDPRGRRVSRTESGSLEPGRHELAWDGRDDSGDTVPAG
                     IYFVRVVAGNQADERKIVRVR"
     gene            596..1084
                     /locus_tag="VF720_07365"
     CDS             596..1084
                     /locus_tag="VF720_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879213.1"
                     /translation="MTRKLIAIVMVGAAIAAGTIGIATSREPALAVPGAATPAGDAEA
                     ARTPQPIDPEHLTPMHREIQAYLQEERLQVEALTGRLAAASTEERLTLHLQIEQAKKA
                     GQRGVFEIQLRHARAAGNIESVQQLESVLAHIDRPEPALDGPTLLEQVQRGLLPAAGS
                     TR"
     gene            1273..2265
                     /gene="argF"
                     /locus_tag="VF720_07370"
     CDS             1273..2265
                     /gene="argF"
                     /locus_tag="VF720_07370"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX7879214.1"
                     /translation="MTSLKNRHFLEFADLTPEEARHLLDLAHDLKKNRKLGRGALAGN
                     SIVLIFEKTSTRTRCAFEVAAYELGMGVTYLDPGGSQIGHKESIRDTARVLGRMYQAI
                     EYRGFEQRVVNELAAFAGVPVFNGLTDDSHPTQMLADLMTMEEEASGRPLGELSYAYL
                     GDSRNNMGNSLLLAGALMGMDVRICGPKALWPTDAVRTRANALATDSGARITLTDDPK
                     KAVAGVDYVYTDVWVSLGEPPERWGERIDLLKPYQVNAALMRDTGNPNTKFLHCLPAF
                     HNRETTKGEELFQAYGMDGLEVTDEVFESPASVVFNQSENRMHTIKALLLSVLG"
     gene            2262..3221
                     /locus_tag="VF720_07375"
     CDS             2262..3221
                     /locus_tag="VF720_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEX7879215.1"
                     /translation="MTKTGAGAKPKDDAARRKLRARRGLMDCERLRAKGGKSFRLSDH
                     KTNDHRPFKDKEAADGYLQLGVARLAKLQDVLYAQDRWSVLLVIQAMDAAGKDGTIKH
                     VMSGLNPQGCHVVSFKAPSPAELDHDFLWRCQQHLPERGRIGVFNRSYYEEVLVVRVH
                     PEILAGQKLPPVLVTKNIWKERYEDINAAERYLVRNGTRIVKVFLHVSYEEQGRRMLA
                     RLEDPDKNWKFSAADLKERGYWKDYMKAYQEMIAATSTDWAPWHVVPADNKWFSRLAV
                     ADLLIDTLEGMDLQYPRVNAEKKKELEVIRKKMAAEVGGKSKA"
     gene            3218..3712
                     /locus_tag="VF720_07380"
     CDS             3218..3712
                     /locus_tag="VF720_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019506054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879216.1"
                     /translation="MSEDPGVTSPPAPPTRNSLITLREVTQATVRTICALSVSPEQQR
                     FVSPNSVSIAEAHFTPTAWFRAVYADEVPVGFVMLDERPEWDYVYIWRLLIDQRYQKL
                     GFGRRVMQMVEERVTGRAGVTRLELSCVQEPGGPQPFYESLGYRFEGQYESDGEAILI
                     KPIG"
     gene            complement(3752..4624)
                     /gene="cdaA"
                     /locus_tag="VF720_07385"
     CDS             complement(3752..4624)
                     /gene="cdaA"
                     /locus_tag="VF720_07385"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX7879217.1"
                     /translation="MNAFFDSIGSGIHWLRVHWREAVDLTLVAGIFYYLFRMMRGTRA
                     AQMFVGLVLVVAASLVASWLKLAALNYIADSLRAVWIVVFVILFQPELRGALMSLGKG
                     RLFSAFTRGEEYTILGEIVRAAESLSARHIGALIVVEREVGLKNYVETGTPIEARISH
                     ELLTTVFTPPSPLHDGAAILSGNRLVAAGCILPLSQNPRLIYALGTRHRAALGVSEET
                     DAVVVVVSEETGAISIAERGRLLRNLDPGALRSTLSTFFRLRGAAEEAAVVAAGTPTE
                     VVDTDPASKERIQA"
     gene            complement(4621..>5513)
                     /gene="folP"
                     /locus_tag="VF720_07390"
     CDS             complement(4621..>5513)
                     /gene="folP"
                     /locus_tag="VF720_07390"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX7879218.1"
                     /translation="QRSCRGNPMTSPRPSCDWQLAGGHTLSLGRVTRVMGILNMTPDS
                     FSDGGRHLDRDAALAAARTMVAAGADLIDIGGESTRPGAEPVPPAEQLRRILPLVGDA
                     AARGILVSVDTTSAEVARECLAAGAVAVNDISGLRFEPALADVTREAGAGLILMHSRG
                     TPRDMHHDPRYHDVVTEVVAELHDAAGRALERGVAPEALVMDPGIGFAKSIGHNLELL
                     QNLDRIVAAGRPVLVGVSRKSFIGRILDLGIEERLEGSLAAATAAVMSGAHLVRVHDV
                     AATVRAVRVADAIRNGSPTT"
BASE COUNT          986 a         1864 c         1864 g          799 t
ORIGIN      
        1 gtcccgggct ccggggcgat cgactggggg atcggcctct atccacccgg ccacgcgttc
       61 acgtcgaaga gcggcgtgca tcccggcggc atcgcgttcg acgcgccggt cggcgccacg
      121 gagtcgatga acgtcacgat cagccaaccc ggcctgtaca cggtggtcgt ctggaagaac
      181 cggcccgccg acctcggcaa caccgggacg atgacgctgc tcttcgatcg ccagaccacc
      241 ggagtgccgg atcccggccg ccccctcgga aaccggctca tccagggcgt ccggcccaat
      301 cccgtcatcg gcccggccac ggtggaggtt gaactcaccg cggcgtcgaa cgtggtcctg
      361 tcgatctttg atccgcgtgg ccgccgcgtc agccgcaccg aatccggctc cctcgagccc
      421 ggccggcacg aactggcctg ggacggccgc gatgactcgg gtgacaccgt tccggccggc
      481 atctatttcg tccgcgtcgt cgccggaaac caggccgacg aacggaagat cgtccgcgtt
      541 cgctaggaat cgaaccggcc gccccggcgg ccgatttcag gaggaggtat ccgtcatgac
      601 caggaagctg atcgccatcg tgatggttgg cgccgccatc gccgccggaa ccatcggaat
      661 cgccacgtca cgcgagccgg ccctggccgt tcccggcgcc gccacgcctg ccggggacgc
      721 cgaggccgcg cgtacgccgc aacccatcga ccccgaacac ctcacgccaa tgcaccgcga
      781 aatccaggcg tacctccagg aggagcggct ccaggtcgag gcactcaccg gacgactggc
      841 cgccgcctcc acggaggagc gcctcacgct gcacctccag atcgagcagg ccaagaaagc
      901 cggccagcgc ggcgtcttcg aaatccagct tcgccatgcc cgggccgccg gcaacatcga
      961 atccgtgcag cagctcgagt ccgtgctggc gcacatcgat cgaccggagc ccgcactcga
     1021 tggcccgacg cttctggagc aggtccagcg cggcctcttg ccggcggcgg gatcaacgag
     1081 atagctccga gagccacccg atcaaggcgc caactcgccc cggagcgggt tggcgccagc
     1141 caggtcgtcg gtccggcatt tgatcgcgag attgcgcaat cgagtctcca cggggatggg
     1201 ggagtgtcgt aggatcatgc gttccggcgg cggccggggc agcccgccga cgtccgtttc
     1261 ccgggagtgc gcatgacatc gctcaagaac cgccatttcc tcgaattcgc cgacctgacg
     1321 ccggaggagg cgcgccacct cctcgacctg gcgcacgacc tcaagaagaa ccgcaagctc
     1381 gggcgcggcg cactggccgg caacagcatc gtgctcattt tcgagaagac ctccacgcgg
     1441 acgcgttgcg cgttcgaagt ggcggcctac gagctcggga tgggcgtgac gtacctcgat
     1501 ccgggcggtt cgcagatcgg ccacaaggaa tcgatccgcg acacggcgcg ggtgctcggc
     1561 aggatgtacc aggccatcga gtatcgcgga ttcgagcaac gcgtggtgaa cgagctggcg
     1621 gccttcgcgg gcgtgccggt gttcaacggc ctgaccgacg actcgcatcc cacccagatg
     1681 ctggccgacc tgatgacgat ggaagaggag gcatccggac ggccgctggg cgaattgtcg
     1741 tacgcctacc tcggcgattc acggaacaac atgggcaatt cgctgctcct ggccggcgcg
     1801 ctcatgggga tggatgtccg catctgcggg ccgaaggccc tgtggcccac cgacgccgtg
     1861 cggacgcgcg ccaacgccct ggccaccgat tcgggcgccc gcatcacgtt gaccgacgat
     1921 ccgaagaagg cggtggccgg cgtggactac gtgtacaccg acgtgtgggt cagcctcggc
     1981 gagccgcccg agaggtgggg cgaacggatc gacctcctca aaccctacca ggtgaacgcc
     2041 gccctcatgc gcgacacggg aaacccgaac acgaagttcc tccactgcct gcccgcgttc
     2101 cacaaccggg agacgaccaa gggcgaggag ctcttccagg cctacggcat ggacggcctc
     2161 gaggtgaccg acgaggtgtt cgaatcgccc gcgtcggtgg tcttcaacca gtccgaaaac
     2221 cggatgcaca cgatcaaggc gctgctcctg tcggtgctgg ggtgacgaag acgggggctg
     2281 gagcgaagcc gaaggacgat gccgcacgcc ggaagctgcg cgcccggcgc ggcctcatgg
     2341 actgcgagcg cctgcgcgcg aagggcggca agtcattccg cctgtcggat cacaagacca
     2401 acgaccaccg gccgttcaag gacaaggaag ccgccgacgg ctatctccag ctcggcgtgg
     2461 cgcggctggc gaagctccag gacgtgctgt atgcccagga ccgatggtcc gtactactcg
     2521 tcatccaggc catggatgcg gcagggaaag atggcaccat caagcacgtg atgagcgggc
     2581 tcaacccgca gggatgccat gtggtgtcgt tcaaggcgcc cagcccggcc gagctggatc
     2641 atgacttcct ctggcgctgc cagcagcacc ttcccgagcg tggccgaatc ggcgtgttca
     2701 accggagcta ctacgaggaa gtgctggtcg tccgcgtgca tcccgaaatc ctggcggggc
     2761 agaagctgcc gccggtgctc gtcacgaaga acatctggaa ggaacgctac gaggacatca
     2821 acgcagcgga acgctacctc gtacgcaacg gcacgcgcat cgtgaaagtg ttcctgcacg
     2881 tgtcgtacga ggagcagggg cgccgcatgc tggcccgcct cgaagacccg gacaagaact
     2941 ggaagttctc cgccgccgac ctcaaggaac gcggctactg gaaggactac atgaaggcct
     3001 accaggagat gatcgccgcc acctccaccg actgggcgcc gtggcacgtc gtgccggccg
     3061 acaacaagtg gttctcgcgc ctcgccgtgg ccgatctcct catcgacacg ctcgaaggga
     3121 tggacctcca gtatccgcgc gtgaacgcgg agaagaagaa ggagctggaa gtcatccgga
     3181 agaagatggc ggcggaggtg ggcgggaaat cgaaggcatg agcgaggacc ccggcgtgac
     3241 atcgccgccg gccccgccga cgcgcaacag cctcatcacc ctgcgcgagg tgacgcaggc
     3301 cacggtgcgc acgatctgcg ccctctcggt gtccccggaa cagcagcggt tcgtgagtcc
     3361 caacagcgtg tcgatcgccg aggcacactt cacgcccacc gcctggttcc gcgccgtgta
     3421 tgccgacgag gtgccggtcg gcttcgtcat gctcgacgaa cgccccgagt gggactacgt
     3481 gtacatctgg cggctgctga tcgaccagcg gtaccagaag ctggggttcg gacggcgggt
     3541 gatgcagatg gtggaggaga gggtaaccgg ccgcgccggg gttacccgcc tggagctgtc
     3601 gtgcgtacag gaaccgggcg gtccgcagcc gttctacgaa tcgctgggat atcgattcga
     3661 gggccagtac gaatcggatg gcgaagccat cctcatcaag ccgatcggat gagttcgggg
     3721 gatgggcggc gcatcggcct cgggcgaatg gctacgcctg aatccgctcc ttcgacgccg
     3781 gatccgtatc caccacctcg gtcggggtgc cggcggccac gacggcggcc tcctcggcgg
     3841 cgccgcgcag gcggaagaag gtggagagcg tactgcggag cgcgccgggg tccagattgc
     3901 gcaggagccg gccccgttcg gcaatcgaga tggctccggt ttcctccgac acgaccacaa
     3961 ccaccgcgtc ggtctcctcg ctgacgccaa gcgccgcgcg atgccgcgtg cccagcgcgt
     4021 agatcagccg cggattctgg ctgagcggca ggatgcaccc cgctgccacc agccgattcc
     4081 ccgaaagaat ggccgcacca tcgtgcagcg gtgacggcgg cgtgaacacc gttgtcagca
     4141 gttcgtggct gatgcgcgcc tcgatcggcg tgccggtttc cacgtaattc ttgaggccga
     4201 cctcgcgctc gacgacgatc agcgccccga tgtgccgggc cgacaaactc tcggcggcgc
     4261 gcacgatctc gccgaggatc gtgtactcct cgccgcgggt gaaggcgctg aagaggcgcc
     4321 ccttgccgag cgacatcagg gcgccgcgca gctcgggctg gaagaggatg acgaagacca
     4381 cgatccagac ggcccgcagc gagtcggcga tgtagttgag cgcggccagc ttgagccagc
     4441 tggccacgag gctggcggcc accacgagga cgaggcccac gaacatctgg gcggcgcggg
     4501 tgccgcgcat catccggaag aggtagtaga agatgccggc gacgagggtg aggtcgaccg
     4561 cttcgcgcca gtgaacgcgc aaccagtgga tgccggaacc gatgctgtcg aagaaggcgt
     4621 tcatgtcgtc ggggacccgt ttcgaatggc gtcggccacg cgcaccgcgc ggaccgtggc
     4681 ggccacgtcg tgcacgcgca cgagatgagc gccggacatc acggcggccg tcgccgccgc
     4741 caggcttcct tccagccgct cctcgatccc caaatcgaga attcggccga tgaacgactt
     4801 gcgtgacacg cccaccagga ccggtcgacc cgcggccacg atgcggtcga ggttctgaag
     4861 cagctcgagg ttgtggccga tgctcttggc gaacccgatt cccggatcca tcacgagggc
     4921 ttccggcgcc acgcccctct cgagggcgcg accggcggcg tcgtgaagct cggccaccac
     4981 ttccgtcacc acatcatggt aacgcggatc gtgatgcatg tcgcgcggcg ttccgcggct
     5041 gtgcatcagg atgagaccgg cgccggcctc ccgggttacg tcggcaaggg cgggttcgaa
     5101 ccgcaggccc gagatatcgt tcaccgccac cgccccggct gccaggcatt cccgggctac
     5161 ctcggcgctg gtcgtgtcga ccgacaccag gatgccgcgg gccgccgcgt ctcccaccag
     5221 cggcaggatg cgccggagct gctcggccgg cggcacgggc tcggccccgg gccgggtgct
     5281 ctcgccgccg atgtcgatga gatcggcgcc ggcggccacc atggttctgg ccgccgccag
     5341 cgccgcatcc cgatcgagat ggcgcccccc gtccgagaag gagtcgggtg tcatgttcag
     5401 gatccccatc acccgggtga cacggccgag ggaaagggtg tgcccaccgg cgagctgcca
     5461 gtcgcaggag gggcgggggg aagtcatcgg gttgccgcgg caggaacgtt gga
//
LOCUS       DASVOD010000326         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_284202, whole genome shotgun sequence.
ACCESSION   DASVOD010000326 DASVOD010000000
VERSION     DASVOD010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284202"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1825
                     /locus_tag="VF720_07395"
     CDS             <1..>1825
                     /locus_tag="VF720_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7879219.1"
                     /translation="KVSGELVNGSDQSLDFSFTGLPAWLTPSPADGTIEPGQKVQVDF
                     AIGTNLAIGSWNATVTAVARQGATILFQAPLAIRVDVVCVPPTWALNTAEFEQSMTLI
                     AQVTIAGSPATTKDIVAAFVGNQLRGLGHPQVVAGRPDSLLYLTVYSNRTAGETVRFQ
                     FFDQSACRVFNSGSKTYAFVADSRIGAPTLPEAITAADVPPGSPQTVPINEGWTWISF
                     NRLSPLDMSVNGVLGDLNSSTGDILKSQSAFSQFDEVAGWVGNVSVFNNLVGYMIRSS
                     EAGQVVLEGNPVPPTQTDIPVVTGWNWISYLPTAEIETNLALATLAPVNGDVIKGQDG
                     FAQYVKTGSVSRWFGSLAKLTPGRGYKLFLSNASNTQNKFRYPNPPVAPSTVGIRQDA
                     DLGPLADSGNGPGWTVDPHLFQYNMTVTGTLETDGAAAPAEGDMVAAFVDDELRGTAM
                     LQEFPGDGRRMFFLMIHGNTPSGEAVRLELYNEATDRIYKVDGTVAFEADRVVGNPAL
                     PWIGRVTEQAVEPPVGGNVPQAFSLAQNAPNPPNPLTTIRFAMPKSERVSMNVYDVAG
                     RHVMTLVDGSLGAGWHEVTLDSQMFSSGLYFYRLEAGSFVQT"
BASE COUNT          323 a          634 c          575 g          293 t
ORIGIN      
        1 aaggtgtccg gcgagctggt gaacggctcc gaccagtcgc tcgatttcag cttcacgggg
       61 ctgccggcgt ggctcacccc ctcgccggcc gacggcacca tcgagcccgg ccagaaggtg
      121 caggtggact tcgcgatcgg cacgaacctg gccatcggtt cctggaatgc cacggtaacc
      181 gcggtggccc gccagggggc gacgatcctc ttccaggccc cgctggcgat ccgcgtggac
      241 gtggtctgcg tgccgccgac ctgggcactc aacaccgccg agttcgagca gagcatgacg
      301 ctcatcgcgc aggtgacgat cgccggaagc ccggccacga cgaaggacat cgtggccgcg
      361 ttcgtcggaa accagctccg gggcctggga catccccagg tcgttgccgg gcggcccgac
      421 tccctcctct acctgacggt gtacagcaac cgcacggccg gcgagacggt ccgattccag
      481 ttctttgacc agtcggcctg tcgtgtcttc aacagcgggt cgaaaacgta cgccttcgtc
      541 gccgacagcc ggatcggagc gcccacgctt cccgaagcga tcaccgccgc cgacgtgccg
      601 ccgggctcgc cgcagacggt gccgatcaac gaaggttgga cgtggatctc gttcaatcgc
      661 ctgtcgccgc tggacatgag cgtgaacggc gttctgggcg acctgaactc ctcgaccggc
      721 gacatcctca agtcgcagtc ggcattcagc cagttcgacg aagtggccgg ctgggtcggc
      781 aacgtgtctg tcttcaacaa cctggtcggc tacatgatcc ggtcgtccga ggccggccag
      841 gtggtgctcg aagggaaccc cgttccgccc acgcagaccg acatcccggt cgtcaccggc
      901 tggaactgga tcagctacct gccgaccgcc gaaatcgaaa ccaacctggc cctggccacg
      961 ctcgcgcccg tgaacggcga cgtgatcaag gggcaggacg gcttcgcgca gtacgtgaag
     1021 accggctcgg tgagccggtg gttcggcagc ctggcgaagc tcactcccgg aaggggatac
     1081 aagctcttcc tctccaacgc cagcaacacg cagaacaagt tccgctaccc caacccgccg
     1141 gtggcgccat ccacggtcgg gatccggcag gacgccgacc tgggcccgtt ggccgattcc
     1201 ggcaacggtc cggggtggac ggtggatccg catctcttcc agtacaacat gaccgtgact
     1261 ggaaccctgg aaacggacgg agccgccgct ccggccgagg gtgacatggt ggccgcgttc
     1321 gttgacgacg agctccgcgg aaccgccatg ctccaggagt tccccggaga cggtcgccgg
     1381 atgttcttcc tcatgatcca cggaaacaca ccgtcgggag aagcggtccg cctcgagctc
     1441 tacaacgagg ccactgaccg catctacaag gtggacggta ccgtggcgtt cgaagccgac
     1501 cgggtcgtcg gaaatcccgc cctcccctgg atcggccgtg tcaccgaaca ggccgtcgag
     1561 cctccggtgg gcggcaacgt tccccaggcc ttcagcctgg cccagaacgc tcccaacccc
     1621 ccgaacccgc tcaccacgat ccgcttcgcc atgccgaaat ccgagcgtgt gtcgatgaac
     1681 gtgtacgacg tggccggtcg ccacgtcatg acgctcgtcg acggctccct gggtgccggc
     1741 tggcatgaag tgacactgga cagccagatg ttctcgagcg gtctctactt ctaccgcctc
     1801 gaggcgggga gcttcgtgca gacgc
//
LOCUS       DASVOD010000327         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_75454, whole genome shotgun sequence.
ACCESSION   DASVOD010000327 DASVOD010000000
VERSION     DASVOD010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75454"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF720_07400"
     CDS             <1..291
                     /locus_tag="VF720_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879220.1"
                     /translation="LSLGRCLNPATILGGVAIEVFVNSIKIKEVGQVSPDVAGTPLGP
                     PCAVALNDAVQFTFPLATNTYSFCFDLNSDGAVGLIDAVALTPPVASAANCN"
     gene            complement(656..1030)
                     /locus_tag="VF720_07405"
     CDS             complement(656..1030)
                     /locus_tag="VF720_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7879221.1"
                     /translation="MVLIVEDEKLLARTLSRAMKDAGYRTVTVGSAELAEKQLGASQF
                     DLMVIDNRLPRETGLELLSKVRGANQELKVILMTAFDSKDVKSEARKLKVDRYLKKPF
                     DLERILAHVSELIGGPEAGPAD"
     gene            complement(1303..>2742)
                     /locus_tag="VF720_07410"
     CDS             complement(1303..>2742)
                     /locus_tag="VF720_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7879222.1"
                     /translation="PSTDARSRGGGLLKSTVLIIDDEPSIVRSLDRVLTDRGYRVESA
                     GSAASGMEKLASVRPQVVLLDLNLPDGHGMPLIKEILVVDPAIRVIVITAFGDLKLGI
                     EAMKAGAADFLKKPYEMDELLLAVDNAARAFESQVRLDIYRRREAEMFHGQHLIGESP
                     PMVRLLDIVERVAGSNATTVLILGESGTGKELVARAVHYGSDRRQSPLMEVNCSAFQE
                     SLLENELFGHEKGAYTDARELKKGLVELCDGGTLFLDEVADMPLATQAKLLRFIENQT
                     FKRVGGAVDLRVDLRIVAATNKDLAAEIAAGNFREDLYYRLKVVSLELPRLSERGDDV
                     LLLARWYLRHFNQKFKKSFREIAPEAGELIQAYQWPGNVRELKNVLERLVLLENDTVL
                     RVSHLPAEIRTAGESPLVRDAGGGGWLEGDGVPTLARIEDDHIHRVLRITGGNKSQAA
                     RLLGISRQGLFERLKRHTEPAPLVERDVK"
BASE COUNT          462 a          918 c          838 g          524 t
ORIGIN      
        1 ctgagcctcg ggcgttgcct caacccggcc accatcctgg gtggcgtggc catcgaagtc
       61 ttcgtcaaca gcatcaagat caaggaagtt ggccaggtgt cgccggacgt ggcgggcacc
      121 cccctcggac cgccttgcgc cgtcgcactg aacgacgccg tgcagttcac ctttccgctg
      181 gccaccaaca cctacagctt ctgcttcgat ctgaactcgg acggcgcggt agggctcatc
      241 gacgcggtgg ccctgacgcc accggttgcg tccgcggcca actgcaactg atcggggccg
      301 ccgtcgtccc gggcggcacc ctgaccattt ccgcggcctg accgcgcgaa ccgactgaaa
      361 acaaggaagg gcggatcctc ccggaggatc cgcccgtctc cgttcccgcc cggacgcggt
      421 ttcccgtcgt caccccatcc cccaagcata aaaagtgggc cggccctgac tccggccagg
      481 accgacccct ctcggtgccc gcccgtcaat gcgggcgggg cgttcgggcg gaagtcgaat
      541 tacttcttct tcgacgcctt cttcgcagcc ttcttcttgc cagtcttctt cgcagccttc
      601 ttcgccgcct tcttcttcgc cgccattacg aaccacctcc ttcccgtttt gggatttaat
      661 cggctggacc agcttccggg ccgccgatca actcggacac gtgggccaat atccgttcga
      721 ggtcgaacgg ctttttcaga tatcggtcca ccttcaattt ccgggcctcg ctcttgacgt
      781 ccttgctgtc gaaggccgtc atgaggatga ccttgagttc ctggttcgcg ccccgcacct
      841 tcgagagcag ctcgaggccg gtctcccgcg gcaggcggtt gtcgatcacc atcaggtcga
      901 actgggaggc acccagttgc ttttccgcca gctcggcact gccgaccgtg acggtgcgat
      961 atcctgcatc cttcatggcc cggctcagcg tgcgcgccag cagcttctcg tcctccacga
     1021 tcaggaccat cttggacacc ttcgcccccc agacatcatt tcccggcatt gtgcaaacct
     1081 cataccaaat caggagttac cagaagtgtg ccagtgaggc aaacccttaa ctgacggagt
     1141 gacagccagt tagaagcccg tggcgtggcg tcgggaagcg aatggggcgg gagcgagcta
     1201 accggcggcg gcgtaatgaa ataggacgat aattggccgg caagcggcaa ccacttgccg
     1261 tataacgact taacggtgcg tcaacaactt agacaagtca aattatttga cgtcccgctc
     1321 gacgagcggg gccggttcgg tatggcgctt gagccgttcg aagagcccct ggcgcgaaat
     1381 tccaaggagc cgcgcggcct gggacttgtt gccgccggtg atccgcagga cgcgatggat
     1441 atgatcgtcc tcgatgcgcg cgagtgtcgg taccccgtcc ccctcgagcc accctccccc
     1501 acccgcgtcg cgcacgagcg ggctctcccc ggccgtacgg atctcggccg gcaggtgcga
     1561 gactcgcagg accgtgtcgt tctcgaggag caccaacctt tcgaggacgt tcttcagctc
     1621 ccgcacgttt ccgggccact ggtaggcctg gatcagctcc ccggcttccg gggcgatctc
     1681 gcggaaggac ttcttgaatt tctggttgaa gtgccggagg taccagcggg ccaggagcag
     1741 cacgtcgtct ccccgctcgg agagacgggg gagttcgagc gagaccacct tgagccggta
     1801 gtagaggtcc tcgcggaaat tgccggccgc gatctcggcg gccaggtcct tgttcgtggc
     1861 cgccacgatc cggagatcca cacggagatc aaccgccccc cccacccgct tgaaggtctg
     1921 gttctcgatg aatcggagga gcttggcctg tgtggccaac ggcatgtcgg ccacctcgtc
     1981 gaggaagagg gttccgccgt cgcagagttc caccaggccc ttcttgagct cacgcgcgtc
     2041 ggtgtaggcc cccttctcgt ggccgaacag ttcgttctcg aggagcgatt cctggaaggc
     2101 gctgcagttc acctccatca gtggcgattg cctgcgatcg ctgccgtaat ggacggcccg
     2161 cgccaccagc tcctttccgg ttccgctctc ccccaggatg aggacggtcg tggcgttgct
     2221 gccggccact cgttccacga tgtcgaggag gcggaccatg ggagggctct cgccgatcag
     2281 gtgctggccg tgaaacatct cggcctcgcg ccggcggtag atgtcgagcc gcacctggct
     2341 ctcgaacgcc cgggccgcgt tatccacggc caggaggagt tcatccatct cgtacggctt
     2401 cttcaggaag tcggccgcgc cggccttcat ggcctcgatg cccagcttga ggtcgccgaa
     2461 cgcggtgatc acgatcaccc ggatggccgg atcgaccacc aggatttcct tgatgagcgg
     2521 catgccgtgg ccgtccggaa ggttcaggtc gagcagcacc acctgcggcc gcacggaggc
     2581 gagtttctcc attcccgagg cggccgaacc ggccgattcg acgcgatagc cgcggtcggt
     2641 gagcacccgg tcgagcgacc gaacgatcga aggctcgtcg tcgatgatga ggacggtgga
     2701 cttcaggagg ccgcctcctc gggagcgagc gtccgtggaa gg
//
LOCUS       DASVOD010000328         2063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_284261, whole genome shotgun sequence.
ACCESSION   DASVOD010000328 DASVOD010000000
VERSION     DASVOD010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284261"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="hisS"
                     /locus_tag="VF720_07415"
     CDS             complement(<1..865)
                     /gene="hisS"
                     /locus_tag="VF720_07415"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX7879223.1"
                     /translation="MKFQAPRGTQDILSPAVEGWQALEGTARRVMARYGYAEVRTPIF
                     ESTELFTRGIGEATDIVQKEMYTFSDRKGRSLTLRPEGTAPVVRAYIEHNLGARRMPY
                     RGWYIGPIFRYERPQAGRYRQHHQVGAELLGSPHPEADFEAIDLFVTLLGAIGLSEVT
                     VKLNSVGDEKSRPAFREILKESLRPRLSELCGDCQRRFEQNVLRVFDCKVPNDRAIVA
                     TLPTMVEHLDPASKEHHDRLRELLLAADIKVEEDPRLVRGLDYYTRTVFEVHHPKLGA
                     QSAVGGGGRYDN"
     gene            complement(862..1329)
                     /locus_tag="VF720_07420"
     CDS             complement(862..1329)
                     /locus_tag="VF720_07420"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEX7879224.1"
                     /translation="MPSRSDSPPSAPGWRRRLEDWSPLFLYTALIVTLSSIPTLAPPT
                     GGVVTDKAYHLVEYAGWALLFRRALDRGRPVTTRRPLKIAVSVILGALLAIADENFQR
                     TVGRQYAVTDMAADMLGVVLAQPVYEILSARVRTARRQAMQRTPDDSGEMSTS"
     gene            complement(1413..>2063)
                     /locus_tag="VF720_07425"
     CDS             complement(1413..>2063)
                     /locus_tag="VF720_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879225.1"
                     /translation="MGDMNRTPALPAIVLTLVVVVLGVALSRKPPVTERLVTLGVGEV
                     VEIDRRLHHRWQLTALSTDRDGRRTLFLKSLGGGKGGDVSTTLRLEEGTPLAWDTFEI
                     SLRDVRLDPARGTAVQLQWVPGDSTKPRPLFSVSDEEPVTLPADLLATIEVEGPIPPP
                     GGGVLTLVIRRPGAEPMTGRIERGSPFPVGKYGALTWIGTSERWLTTLAIRSRRPA"
BASE COUNT          318 a          701 c          713 g          331 t
ORIGIN      
        1 ggttgtcgta acgcccgccg ccgcccaccg cgctctgcgc gcccagcttc gggtggtgca
       61 cctcgaacac cgtgcgcgtg tagtagtcga ggccccgcac gagccgcggg tcttcctcga
      121 ctttgatgtc ggcggcgagg aggagctcgc gaagccggtc gtggtgttcc ttcgacgccg
      181 gatcgaggtg ctcgaccatc gtcggaagcg tggccacgat cgcccggtcg ttcggaacct
      241 tgcagtcgaa gacgcgcagc acgttctgct cgaagcgccg ctggcagtcg ccgcacagtt
      301 ccgacaggcg cggccggagc gactctttca gtatttcgcg gaaggccggc cggctctttt
      361 catcccccac cgagttgagc ttcacggtga cttcggaaag cccgatggcc ccgagcagcg
      421 tcacgaagag gtcgatggcc tcgaagtcgg cttcggggtg aggcgatccg aggagctcgg
      481 cgccgacctg atgatgctgg cgatagcggc cggcctgcgg ccgctcgtaa cggaagatgg
      541 ggccgatgta ccagccgcgg tacggcatgc gccgggcgcc gaggttgtgt tcgatgtagg
      601 cgcgcaccac cggcgccgtc ccctcggggc gcagcgtgag gctccgcccc ttccggtcgg
      661 aaaaggtgta catctccttc tggacgatgt cggtggcctc gccaatgccg cgcgtgaaga
      721 gctcggtcga ttcgaagatc ggggtgcgca cttcggcgta accgtaacgc gccatgaccc
      781 ggcgggccgt tccttccagg gcctgccacc cctcgacggc cgggctcagg atgtcctgcg
      841 tgccgcgtgg cgcctggaat ttcatgacgt cgacatctcc ccggaatcgt ccggtgttcg
      901 ctgcatggcc tgccgccggg ctgtccggac gcgggccgaa agaatctcgt acacgggctg
      961 ggccagcacg acgcccagca tgtcggcggc catgtcggtc accgcgtact ggcggccgac
     1021 cgtccgctgg aagttctcgt cggctatcgc gagaagcgcc cccaggatta cgctgaccgc
     1081 gatcttgagc ggtcgccggg tcgtcaccgg ccgcccgcgg tcgagtgccc gccggaagag
     1141 gagggcccac ccggcatact ccaccaggtg gtacgccttg tcggtgacga ccccccccgt
     1201 cggcggcgcc agcgtcggga tcgacgagag ggtgacgatc aatgccgtgt acaggaagag
     1261 cggcgaccag tcctcgagcc gacgccgcca ccccggtgcc gagggcgggc tgtccgaacg
     1321 tgatggcaaa gtggcgtcgc tccccggcac aagcctttca agagcgggcg attatgccac
     1381 gagtgcgcgc cgccgtgtca ggggtcgggc ggtcaggccg gacggcgcga acggatcgcg
     1441 agggtggtga gccaccgttc gctggtgccg atccaggtga gggcaccgta cttgccgacg
     1501 gggaacgggc tccctcgctc gatccttccc gtcatcggct cggcgccggg cctccggatg
     1561 accagggtga ggacgccgcc tcccggcggc ggaatcggac cctcaacttc gatcgtggcc
     1621 aggagatcgg cggggagcgt caccggttcc tcgtcgctca ccgaaaagag gggccgcggc
     1681 ttcgtgctgt cccccggaac ccactggagc tgcaccgccg tgccccgcgc ggggtccagg
     1741 cgcacgtcgc gcagggaaat ctcgaatgtg tcccatgcca ggggtgttcc ttcctccagc
     1801 cgaagggtcg tcgagacgtc gccacccttg ccgccgccaa gcgacttgag gaagagggtc
     1861 cgccggccat cccgatccgt gctgagggcc gtaagctgcc accggtgatg aagccggcga
     1921 tcgatctcga ccacttcccc cacacccagc gtgacgagac gctcggtgac cggcggcttg
     1981 cgggataggg cgacgcccag cacgaccacg accagtgtca ggacgattgc cggcagcgcc
     2041 ggcgtccgat tcatgtcacc cat
//
LOCUS       DASVOD010000329         1606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_231670, whole genome shotgun sequence.
ACCESSION   DASVOD010000329 DASVOD010000000
VERSION     DASVOD010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231670"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1105
                     /locus_tag="VF720_07430"
     CDS             143..1105
                     /locus_tag="VF720_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX7879226.1"
                     /translation="MSRSSASGGLRASIDRFIIDLLAGRGFSPRTAEAYRRDLGAWIA
                     WLAAREEGEPPAASRLQPAHVTLYLADLSKRGRSARTLARHLSAIRSYARWRQRHGEE
                     ATFTAGLRGPRLPKPMPAFLTEAEMERVFHLDFGEGNAALRDRAVLELLYASGIRLAE
                     LVSLNRDDAVDLDRGQVRVLGKGNRERIVPIGREAVRAMSAYASATAGVASADRDGLP
                     FFLGTGGRRLSRRTVQRLIAGHLGRVASRSGLSPHLLRHTVATHLLARMSREGSGKGS
                     SAPDIRAVQELLGHVSLSSTQVYTHITVDRLRRAVTVAHPRGEE"
     gene            1102..>1606
                     /locus_tag="VF720_07435"
     CDS             1102..>1606
                     /locus_tag="VF720_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7879227.1"
                     /translation="MRSATGWILALALLALAGCAEIGPPPGGPPDLVPPYVLAARPDS
                     LATRVDPAAEIALTFSEKVERRTLREWIGVNPYRPIKSFHWDGPTIFFELAGGLPADT
                     TVQVYIGSGVTDRAGLPMYPLFSRLFTTGDSIAPGVIEGKLRASRLATATAAPGSAPG
                     HVPLSALG"
BASE COUNT          232 a          584 c          544 g          246 t
ORIGIN      
        1 ggtggagtgc ccgcagtgcg gcaacccctt catgtcgcag aaggaatcga agtcgaaggg
       61 gtcgttcctc cgctgtccga agtgcaaggc ggaactgtcg agcggcgaga ccgccgactg
      121 acgccatgac gcgcggggac cggtgagccg atcgtccgct tcggggggcc ttcgcgcctc
      181 gatcgaccgc ttcatcatcg acctcctggc cggacgaggc ttctcgccgc gcacggccga
      241 ggcgtaccgc cgcgatctgg gcgcctggat cgcctggctt gccgcgcgcg aggagggaga
      301 gccgccggcc gcctccaggt tgcagccggc ccacgtcacc ctctacctgg ccgatctctc
      361 gaagcgcggc cgttcggcgc gcacgctggc ccggcacctc tccgccatcc gcagttacgc
      421 ccgctggcgg cagcgccacg gcgaggaggc caccttcacc gcgggactca ggggtcccag
      481 gctgccgaaa ccgatgccgg ccttcctcac cgaggcggag atggagcgtg tcttccacct
      541 cgacttcggc gagggaaatg ccgcgctgcg tgatcgcgcc gttctagaac ttctctatgc
      601 atccggcatc cgactcgccg aactggtcag cctcaatcgc gacgacgcgg ttgatctgga
      661 ccgggggcag gtgcgtgtgc tcggcaaagg gaatcgcgaa cggatcgtgc cgatcgggcg
      721 ggaagccgtg cgggccatgt cggcctacgc ctcggcaacc gccggggttg ccagcgccga
      781 tcgcgatggc ctccccttct tcctcgggac cggaggacgg cgtctctccc ggcgcaccgt
      841 gcagcgcctc attgccgggc acctcggacg agtggcttcg cgcagcggac tctcgccaca
      901 cctgttgcgc cataccgtgg ccacgcacct cctggcccgc atgagccgcg agggaagcgg
      961 gaagggatcg tcggcgccgg acatccgggc cgtccaggaa ttgctcggcc acgtaagcct
     1021 gtcgagcacg caggtctaca cgcacatcac cgtcgaccgg ctgcgccggg cggtcacggt
     1081 ggcgcatccg aggggagagg agtgaggtcc gccaccggct ggatccttgc cctggcgctg
     1141 ctggccctgg ccgggtgcgc cgagatcgga cctccgcccg gcggaccacc cgatctcgtc
     1201 ccgccgtacg tcctggccgc gcggccggat tcgctggcca cgcgcgtcga ccccgccgcc
     1261 gagatcgcgc tcaccttttc cgaaaaggtc gagcggcgca ccctgcggga gtggatcggg
     1321 gtgaacccat accggccgat caagtcgttc cactgggacg gcccgacgat cttcttcgaa
     1381 ctggcgggcg gcctgccggc ggataccacg gtgcaggtct acatcgggag cggcgtcacc
     1441 gaccgtgccg gcctgccgat gtacccgctc ttctcgcggc tcttcacgac gggtgattcg
     1501 atcgcgcctg gcgtgatcga agggaagctg cgcgccagcc gcctcgcgac cgcgacagcc
     1561 gcgcccggct ccgcacccgg ccacgtgcct ctatccgccc tcggac
//
LOCUS       DASVOD010000330         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_335231, whole genome shotgun sequence.
ACCESSION   DASVOD010000330 DASVOD010000000
VERSION     DASVOD010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335231"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..2570)
                     /locus_tag="VF720_07440"
     CDS             complement(3..2570)
                     /locus_tag="VF720_07440"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879228.1"
                     /translation="MSRPGPTGRRQRLSVVVLATPESTRLLPTLESVRDWADEVVVLD
                     ESPQGAWRGITRRLGVRWFDGADNHDIPEARRRALYQTDGEWVLLLLADERLDWRQGG
                     GLLRELVHPDPAPAAWQVRIVDQLDPREPAVPRPAARLVRKRPGLTVADGDQGPALVL
                     HDRPLEESAAPSALWVWCSPVAMPAGDDPVLRSLAEWRDGNRLGALTRLRDLVDNGTE
                     LPPDERARGARLLSSLLVAERKLDEAVDRIESELASVDPGADPALAGFLLGELGRAEL
                     LLDHRTRADACYERGLMLAPRLYALHAGRAEFFEDAGRTTEAIDEWLRAAVVRPDLGT
                     PRRRAGELLGNLGRDDDGLSLLIQAETLDQSLSLASSWIDSADEPVEEPPVTNPMAEL
                     MREFQLTTPATRPGATVEVEHDASGAVEEEAGPVPGPTPVPGPTPGPEIGITPDAMDQ
                     AWDAVTTVGERIERPGAGERVAGEDDAASIDESSDDSSRVTAALVMPVTTESDEVMPP
                     AHPVLPGHRQLSDPSDPAQLERRHLENVAHYEEALAADPDDHELHLKLGLTWFSLREW
                     SKAEPVLRRAVRQTGKALQRPQLAVAWCRLAECSLERGDLVEASREAGSALALDSNSA
                     EARLVLGRIAFRQHRFHEASREFRRLLALPNETLPVDRSVLLREMGIALYRDQRHAEA
                     AEALVKAAERLDGDAMLHLFLGNAQAHIGRTEAAIEAFRTAHRLDPSLPEARNNLVVV
                     TFDLANRYLDEGRHRDVLDLVAGAPGTPELIFLSAVARHALGDLATARRELEALNAMD
                     DTFAEAHWNLAVICRELGDLPAALKTLARFRHLAPTDPRGRTLEEALKRGPANTSVS"
     gene            2663..3766
                     /locus_tag="VF720_07445"
     CDS             2663..3766
                     /locus_tag="VF720_07445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879229.1"
                     /translation="MSKERTKLPWTAEPYRPAERQAILDLIRSYYGPIETADGDFFDW
                     QYFANPSGPVVLWLAHDPAGAIVGQHVLLPMKLLVDGAPSTGCLSFNALVDPRYHGQG
                     IWTGLGRKAHDEAASRGLSLAYGIPNPNSNRPMVKDLGYHRPFDVPVLVKALDLGAAA
                     RHVLGGWKGSVGGFGGSLAGPLLFPRRKPRPGGAAVTQPSRFDAAYDRLAERSGRRYR
                     ILLHRDAAHLNWRYVDCPTRTTHRWEVRSGGESLGYAVLRVTDFRGIKLGVLLDFLVD
                     DSTEAKAAGLEILAHAEEAAREAGCPLLLTFCLSHTAEYGLLHRAGWRVGPAALLPQP
                     FPLLVRPLNWPDSAPTVDVVRHWFLTTGDYDVF"
BASE COUNT          575 a         1415 c         1345 g          630 t
ORIGIN      
        1 gttcaggaaa ctgaagtgtt tgccggcccc cgtttcaggg cctcctcgag ggtgcggccg
       61 cggggatctg tcggggccag gtggcggaag cgggccagtg tcttcagggc cgccggcagg
      121 tcgcccagtt cccggcagat caccgccagg ttccagtggg cctcggcaaa ggtatcgtcc
      181 atcgcgttca gcgcttccag ctcgcggcgc gcggtggcca ggtcgcccag ggcatgccgg
      241 gccaccgccg acaggaagat gagctccggc gttccgggcg cgcccgccac caggtcgagc
      301 acgtcgcgat gacgcccctc atcgaggtat cgattggcca ggtcgaacgt gacgaccacc
      361 aggttgttgc gcgcctccgg gagcgaggga tcgaggcgat gcgccgtccg gaacgcctcg
      421 atggccgcct cggtacgccc gatgtgagcc tgcgcgttgc cgaggaagag gtgaagcatg
      481 gcgtcgccgt cgagtcgctc ggcggccttc accagggctt cggccgcttc ggcatggcgc
      541 tggtcccggt agagcgcgat ccccatttcg cggagcagca cgctccgatc gaccggcagc
      601 gtttcgttgg ggagcgccag gagccggcgg aactcgcgcg aggcctcgtg aaagcggtgc
      661 tggcggaagg cgatccgccc gagcacgaga cgcgcctcgg cgctgttcga atcgagggcc
      721 agcgcgcttc cggcctcgcg cgaggcttcg accaggtcgc cccgctcgag cgaacactcg
      781 gccagccggc accaggccac cgccagctga ggccgctgga gtgccttgcc ggtctggcgc
      841 accgcccgcc tgaggacggg ctcggccttc gaccactcgc gcagcgagaa ccaggtgagt
      901 ccgagcttca agtgcagttc gtgatcatcc ggatcggccg ccagcgcctc ttcatagtgc
      961 gcaacattct cgaggtggcg ccgttccagt tgagccggat cggatggatc gctgagctgt
     1021 cgatgtcccg gcaggaccgg gtgggccggc ggcatcacct catccgactc cgtggtcacc
     1081 ggcatcacca gggccgccgt cacccgggac gagtcgtccg acgattcgtc gatcgacgcc
     1141 gcatcgtctt ccccggccac tcgctccccg gcccccggtc gctcgatccg ctcgcccacg
     1201 gtcgtcaccg cgtcccacgc ctggtccatc gcgtccggag tgatcccgat ctccggcccg
     1261 ggcgtcggtc cgggcacggg cgtcggtcct ggcacggggc cggcttcctc ctccaccgcg
     1321 ccactcgcat cgtgctccac ctcgaccgtc gcgccgggtc gggtggcggg agtggtgagc
     1381 tggaattcac gcatcaattc ggccatcggg ttggtgaccg gtggttcttc caccggctcg
     1441 tccgcgctgt cgatccagct cgaggccagg gagagcgact ggtcgagcgt ctcggcctgg
     1501 atcaggagcg agaggccgtc gtcgtcgcgg ccgaggttcc ccagcagttc gccggcccgg
     1561 cggcgcggcg ttcccaggtc gggacgcacg accgcggcac ggagccattc gtcgatcgcc
     1621 tcggttgtcc ggccggcatc ctcgaagaac tccgcccgcc cggcgtggag tgcgtagagt
     1681 cggggcgcga gcatcagccc gcgctcgtag caggcatcgg cgcgcgtccg gtgatcaagc
     1741 aggagttcgg cccggccgag ctcaccgagc aggaaacctg ccagggccgg atcggcgccc
     1801 gggtcgacag aggccagctc ggattcgatc cggtcgaccg cctcgtcgag cttccgctcg
     1861 gccacgagga gactcgacag gagacgggcc ccgcgcgcgc gttcgtccgg cggcaactcg
     1921 gtgccgttgt ccacgaggtc gcggagtcgc gtgagcgccc ccagccggtt tccatcgcgc
     1981 cactcggcca gtgaacgaag taccgggtcg tctcccgccg gcatcgcaac cggggaacac
     2041 cacacccaca gcgcggaagg cgccgccgac tcttccagcg gccgatcgtg cagcaccagc
     2101 gccggtccct ggtcgccgtc ggccaccgtc agtcccgggc gcttgcgcac gaggcgcgcc
     2161 gccgggcggg gcaccgccgg ctcccgcgga tcgagctggt cgacgattcg tacctgccag
     2221 gccgccggcg ccggatcggg gtgaaccagt tcccggagaa ggccacctcc ctgccgccag
     2281 tcgagccgtt cgtcggcaag gaggaggagc acccattcgc cgtcggtctg gtagagcgcg
     2341 cgacgccggg cctcgggtat gtcgtggttg tccgcgccat cgaaccagcg gacacccagc
     2401 ctgcgcgtga ttccccgcca ggctccctgc ggactttcgt cgagcaccac cacctcgtcg
     2461 gcccagtcgc gcacgctctc gagtgtcggc aacaggcgcg ttgactcggg tgtggccagg
     2521 acgacgacgg acaggcgctg gcgacgcccg gtagggccgg gacgggacaa gggggttctc
     2581 cggaatcggc ggtgcgcggc gccgtagcgc gccaacgccg cattctacac gtgcgcggtc
     2641 gggacgtgtt agtttccggc ccatgtcgaa ggagcggacg aaacttccct ggactgccga
     2701 gccctatcgt cccgccgaac gacaggccat cctggatctc atacggtcgt actacggtcc
     2761 catcgaaacc gccgacggtg atttcttcga ctggcagtac ttcgccaatc cctcggggcc
     2821 cgtcgtcctc tggctggcgc acgatccggc cggcgccatc gtcgggcagc atgtcctcct
     2881 cccgatgaag ctgctcgtcg acggcgcgcc ctccacgggg tgcctctcgt tcaacgcgct
     2941 ggtcgatccc cgttaccatg ggcagggaat ctggacgggc ttgggtcgaa aggcccacga
     3001 cgaggcggcg tcgcgcgggc tgtcgctggc ctacggaatc ccgaatccga actcgaaccg
     3061 cccgatggtg aaagacctcg gctatcaccg gccattcgac gtgccggtgc tggtcaaggc
     3121 gctcgacctc ggcgccgcgg cccgccacgt actcggcggc tggaaggggt cggtcggcgg
     3181 attcgggggg tcgctggccg gtccgctcct ctttccccgc cggaaacctc ggccgggtgg
     3241 cgccgccgtc acccaaccat cccgcttcga tgccgcctat gatcggctgg ccgagcggag
     3301 cgggcggcgc taccggatcc ttctccaccg cgacgccgcc cacttgaact ggcgctatgt
     3361 cgactgcccc acccgcacca cccatcgctg ggaggtgcgt tcgggcgggg agtcgctcgg
     3421 ctacgcggtc ctgcgcgtca ccgacttccg cggcatcaag ctcggcgtgc tgctcgactt
     3481 cctggtcgat gattccaccg aagccaaggc ggccggcctc gagatcctgg cccacgccga
     3541 ggaggcggcc cgcgaggcgg ggtgcccgct gctcctcacg ttctgccttt cgcacaccgc
     3601 cgagtacggg ctgctccacc gggcgggatg gcgggtgggc ccggccgcgc tgttgcccca
     3661 gccgttcccg ctcctggtgc ggccgctcaa ctggccggac agtgcgccca cggtggatgt
     3721 ggtgaggcac tggttcctga ctacggggga ttacgacgtc ttctagggca gcctcatcgc
     3781 aatgcggtca tcaacaataa gaaatatcat tccgaagtcg ttgtgacatt tcggtacgac
     3841 ttgaatttcg gcgtccaatg atcaggcagc cgaagcgcgt cccggtgtcc cctcctccac
     3901 cacttccggc accaccaccg cacacgcccc caccgccgcg aagaagatga agcacgcctc
     3961 gaagg
//
LOCUS       DASVOD010000331         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_493180, whole genome shotgun sequence.
ACCESSION   DASVOD010000331 DASVOD010000000
VERSION     DASVOD010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493180"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF720_07450"
     CDS             <1..248
                     /locus_tag="VF720_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7879230.1"
                     /translation="AASGTDRAGGDRAAADRTAESGATGTAAPVAVLPYEEMEREYIR
                     SVLKVTEGTKKQAAALMGIPRSTLNDRMRKLGLDATG"
     gene            501..1100
                     /locus_tag="VF720_07455"
     CDS             501..1100
                     /locus_tag="VF720_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HEX7879231.1"
                     /translation="MVTEWQAAAVATALMMGSIISTGTAQASGCQDTNAQELSFQTLE
                     HYAVGPYMAGASILVQSAAEWNEQFDEMAADGKLSMYPVDAPSVDWGNEYVIVVAMGE
                     CPSLGYDIEIEHVWQESDGLVIGVRSVHPNGATQAALTSPYHMISIKATDVTKISLCD
                     SGAPKPRIPVRLALPGDDRANESARITVTWSSLKSHVAS"
     gene            complement(1180..2271)
                     /locus_tag="VF720_07460"
     CDS             complement(1180..2271)
                     /locus_tag="VF720_07460"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7879232.1"
                     /translation="MARAQRRNNDSDTFNSYRDDSDRERRDWSSDWDRDNRREDRDRE
                     FYRFEDLGRGTDDDWFDRDRERGGNRDRQSGNRESRNFGRTSDWRSEREYDRGEFGNY
                     GGEFGNYDRDNRDLESGWQGGRDQNRRGNQWSSRTRENRGYQGEGFDDRGDYSPMNRY
                     GQDFGRQSFEGFGQSRNRQFQNQGQYGQSGQGQYGQSGQGQYGQSGSSGQYGQSGQGQ
                     YGQHGNQGSRFGQPMSGEYGSSQGSMDRSHAGVGPKNYKRSDERIKEDLCEMFSANPW
                     LDASNTEVEVKQGVVTLTGTVGDRRMRQMMEDIADGVPGVKDVECQIKLETKTKAGAM
                     GTGEKSGEKDKEKGGNSGSSTHDAGKNRQ"
     gene            2748..3335
                     /locus_tag="VF720_07465"
     CDS             2748..3335
                     /locus_tag="VF720_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879233.1"
                     /translation="MKKFLTVALAIVALAIGHLEASAQMGTSNGTIVSSTPTAIVIRT
                     DDGTNRTYTVNSSSIMPAASLRVGDRIALDYNTLTAGDMVVTRVTVVPGAAVTTTTTS
                     PGYSTSGNTTTNSTTSTTTGSNTTYGSTNGATTGSNTTYGSTTGATTGSNTTYGSTSS
                     TNDTYPDTAANDVLPLLLGGIGLAGAATLRILKRS"
     gene            3396..4241
                     /locus_tag="VF720_07470"
     CDS             3396..4241
                     /locus_tag="VF720_07470"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HEX7879234.1"
                     /translation="MHPALKPPARNRMFVWLERLLLAASMVGVAAWAATAHERVRFQD
                     EARRRLDERTELAAARRGDLSPGALLAVWHDSLAARLSGANAADFDLDSRGTSDFRGA
                     ADFHGAADFHGTSDFDGLADPAPVRPAPDEPFGTLEIPRLGMDVVIVEGTSRKALKNA
                     IGHLETTALPGERGTAAIAGHRDTFFRPLKDIALSDTLAVVTPECRIEYVVDSLLVLK
                     PGETEFLNAPNGNRLVLITCYPFYYVGNAPSRFIVQARPLPERSGGRPEGVGESERAS
                     TPSGG"
BASE COUNT          751 a         1531 c         1370 g          776 t
ORIGIN      
        1 ccgccgcgtc cgggaccgat cgagcgggcg gggatcgggc cgccgcggat cggaccgcgg
       61 aatcgggcgc gaccgggacc gccgcccccg tggccgtcct cccctatgag gagatggagc
      121 gcgagtacat ccgctcagtc ctcaaggtca ccgaggggac caagaagcag gcggccgccc
      181 tgatggggat cccccgctcc actctcaacg atcggatgag gaagctgggt ctcgacgcga
      241 cggggtgacg acccgtcgca gccggtcccc gccgggtgac ggccggccgc cctgacccgg
      301 cacggtatcc cgcccgtgcc ggtgcaccga caccctgtcc acccccgacg gttcgccggc
      361 ccatccctat aaaacactga tttgaaaaca gatacaggat caaccgcgcg cgcctccgcg
      421 ttggcatccg gcttgcgatg gtttcccgcg tctggcaaat gattcacgaa cgacgagggg
      481 ggttcgaagt gaaggttcgg atggttaccg aatggcaggc cgccgcggta gcaaccgcgt
      541 tgatgatggg ttcgatcatt tccaccggca ccgcccaggc gtccggatgc caggacacca
      601 acgcgcagga actctccttc cagactctcg agcattacgc ggtcggtccc tacatggccg
      661 gcgcctcgat cctggtccag agcgcggccg agtggaacga gcagttcgat gagatggccg
      721 ccgacggcaa gttgagcatg tacccggtgg atgccccctc ggtggattgg ggcaacgagt
      781 acgtcatcgt cgtggcgatg ggtgagtgcc cgagcctcgg ctacgacatc gagatcgagc
      841 atgtctggca ggagagcgac ggcctcgtca tcggtgtccg ctcggttcat ccgaacggcg
      901 ccacgcaggc ggccctcacc tcgccgtatc acatgatcag catcaaggcc acggacgtca
      961 ccaagatcag cctgtgcgac agcggtgcgc cgaagccccg catcccggtc cggcttgcgc
     1021 tgcccgggga cgaccgtgcc aacgagtcag cccgcatcac cgtgacctgg agctcattga
     1081 agtcccacgt tgcatcctga gggggcacca gtcacatccc aggggggttc gggccaaacc
     1141 ggagcgctgt gagcatcgcc gcagcgctcc ggttcctttt tactggcggt tctttccggc
     1201 gtcgtgggtg gaggaaccgg agttcccccc cttctccttg tccttctctc ccgacttctc
     1261 gccggttccc atcgcgccgg ccttggtctt cgtctcgagc ttgatctggc attcgacatc
     1321 cttgacgccc gggacgccgt cggcgatgtc ttccatcatc tggcgcatcc gtcgatcacc
     1381 caccgtaccg gtcagcgtga caacgccctg cttcacctcg acctcggtgt tcgaggcatc
     1441 gagccaggga ttggcggaga acatctcaca gagatcttcc ttgatccgtt cgtcggagcg
     1501 cttgtagttc ttcggcccga ccccggcgtg agagcggtcc atgcttccct gcgaggagcc
     1561 gtactcgccg ctcatcggct ggccaaagcg cgaaccctgg ttgccgtgct ggccatactg
     1621 cccctgaccg gactggccgt actggcccga gctgccggac tgcccgtact gtccctggcc
     1681 ggactgaccg tactgaccct ggccggactg cccgtactga ccctggttct ggaactggcg
     1741 attccgggac tggccgaatc cctcgaatga ttgccggccg aaatcctggc catagcggtt
     1801 catgggggag taatcgccgc gatcgtcgaa gccctcgccc tggtagccgc ggttttcgcg
     1861 cgtgcggctg ctccactggt tgccgcggcg attctggtcg cggccgccct gccagccgga
     1921 ctcaagatca cggttgtcgc ggtcgtagtt gccgaattcg ccgccgtaat tgccgaactc
     1981 gccgcgatcg tactcacgct cgctgcgcca gtccgaggtg cggccgaagt tgcgggactc
     2041 gcggttgccg ctctgacggt cacgatttcc accgcgctcg cggtcgcgat cgaaccagtc
     2101 gtcgtcggta ccgcgcccca agtcctcgaa ccggtagaac tcacgatcgc ggtcctcacg
     2161 acggttgtcg cggtcccaat cggacgacca gtcgcgccgc tcacggtcgc tgtcgtcgcg
     2221 gtagctgttg aaggtgtcgg aatcgttgtt ccgacgctga gcacgggcca tttcttacct
     2281 cctggttcgg ttgactgccg ggaatggatg ccgttgtggc gatgcgtcgg ttcgcatcaa
     2341 tcgtgcccgc gcgcccgggc aggggaagcc ccaccgtgcc gcggaattcg tcgacaatga
     2401 ttcgttctgc gacgcatcca ccggcaatcg cgatgcggtg aacgaggctc ccgaaatggc
     2461 tcacaaacag gatgaaattg ttgcaatcga cagttcgact ttcttaccgg ggcccttcgg
     2521 cgcctggaga attgttcgcg acgcgtcggg ggaattgcct gcgcgggcga tggggggatc
     2581 cccggcgaga ggcctgtccc gcaaggactt gggtatcgcc acacctggca ggccgggccg
     2641 cggcgttgac gcggcccgtg gcacctccct tgcaatccac gaaccggtag ccagttgtca
     2701 tggccggacg tgcccgcgac cgccggtcat ctatccagga ggattccatg aagaagttct
     2761 tgacggtcgc gctcgccatc gtggcgcttg cgatcggcca cctcgaagcc tcggcccaga
     2821 tgggaacgag caacggcacc atcgtgtcgt ccacgcccac ggccatcgtc atccgcaccg
     2881 atgacggcac caaccggacc tacaccgtga acagctcgag catcatgccg gcagcctcgc
     2941 tgcgcgtggg cgaccgcatc gcgctcgact acaacaccct gaccgccggt gacatggtgg
     3001 tcacgcgcgt caccgtggtg cccggagccg cggtaacgac gacaacgaca tcgccgggct
     3061 attcgacatc gggcaacacc acgaccaaca gcacgaccag taccacgacc ggcagcaaca
     3121 ccacctacgg cagcacgaac ggcgccacga ccggcagcaa caccacctac ggcagcacga
     3181 ccggcgccac gaccggcagc aacaccacct acggcagcac gagctcgacg aatgacacct
     3241 atcccgacac ggctgcgaac gacgtccttc cgctcctgct cgggggcatc ggtctcgccg
     3301 gcgcggcaac gctgcgcatt ctgaagcgaa gctgacagtc acttgcggtg cggggtcggt
     3361 catcggcatg accgccccgc cccgttggag gtcgcatgca tcctgccctg aaaccaccgg
     3421 cacggaacag aatgttcgtc tggctggagc gcctgctcct cgccgcctcc atggtcggcg
     3481 tggccgcctg ggccgccacc gcgcatgaac gagtccgctt ccaggacgaa gcccgtcgcc
     3541 ggctggacga gcggaccgaa ctcgcggcgg cgcgtcgggg cgatctgtct cccggtgcgc
     3601 tgctggcggt gtggcacgac tcgctggcgg ccaggttgtc cggcgccaac gccgctgatt
     3661 tcgatctcga ctctcgtggg acttcagact tccgtggggc ggcagatttc catggagcgg
     3721 cagacttcca tgggacgtca gatttcgatg gcctggcgga ccctgctccc gtccggccgg
     3781 cacctgacga accgttcggc accctcgaga ttccgcgcct gggcatggac gtcgtgatcg
     3841 tcgaggggac atcccgcaag gcgctgaaga acgcaatcgg tcacctggaa acgacggcgc
     3901 tccccggtga acggggcacc gcagccatcg ccggccaccg ggacactttt ttccgtcccc
     3961 tcaaggacat cgcgctttcg gacacgctcg cggtcgttac gccggagtgc cggatcgagt
     4021 acgtggtcga ttcgctcctg gtgttgaaac cgggcgaaac cgaattcctc aacgccccga
     4081 acggcaaccg actcgtcctc atcacctgtt acccgttcta ttacgtcggg aacgctccct
     4141 cccgcttcat cgttcaggcc cgtccgctcc cggagcggtc aggcggacgt ccggaggggg
     4201 ttggcgagtc agagcgagcg tcgaccccct ccggcggcta atcggcaagc gagcgccaac
     4261 tgcgccagga ctccgggcgc cccccgcaac acagcccagg tcacaattcg ttacctgttc
     4321 cggaacaggt accctccggg cgcccccgca acgcatgccc aggtcacaat tcgttacctg
     4381 ttccggaaca ggtacattcg atgacccgtg atcatcaccc tggccgat
//
LOCUS       DASVOD010000332         9294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_648626, whole genome shotgun sequence.
ACCESSION   DASVOD010000332 DASVOD010000000
VERSION     DASVOD010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648626"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF720_07475"
     CDS             complement(<1..576)
                     /locus_tag="VF720_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7879235.1"
                     /translation="MKRRPVNRERPLPVPPPEVGSRNGVRREPPGTPRGASPEPPAWA
                     RNAVIYSAYPRAFSPEGTLRGVADRVGAIAELGADILWLLPIHPIGESARKGRLGSPY
                     AIRDYRAINPEYGRKDDLLALVRAAHDAGLKLILDCVFNHGAPDHVEAASHPDWFQRD
                     ARGRPTRRVADWHDVVDWDFSAPGLEEHLLES"
     gene            677..1624
                     /locus_tag="VF720_07480"
     CDS             677..1624
                     /locus_tag="VF720_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879236.1"
                     /translation="MRRLVLIFIDGVGVGRPDPDVNPLVGLPILGNTLPQDWHPPLDG
                     GRPASLPDLVREAPLPNGGVARATDPSLGMRGLPQSATGQTTLFTGVNAAERLGHHLY
                     GYPGPTLRKILLESSVLKRATEAGYRAAFLNAYRPLFFDLGEEIWNRPMSASSWNNRA
                     AGLPFMTVDDIRDRRALYHDYTNREPIERGIELPEWSPEEAGRVLAEAAAEHDLAIYE
                     YFLSDRVGHTGEHARARTIAEGLDRFIGSAVGAADLSEQTIVVTSDHGNIDDMTSRSH
                     TMHPVPALVWGPDAPRLAARLDRLEDFAGAMLDHLGSRA"
     gene            1621..2547
                     /locus_tag="VF720_07485"
     CDS             1621..2547
                     /locus_tag="VF720_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879237.1"
                     /translation="MRRAAPPMDVRVVTAFLGAALLPALSGCTATGPMAASSGKSGSI
                     LASRQAGDVPPAPASPIVVTLHALRDTVVAHQTAWVEVRITNNGPGGLDIPDRGQHLV
                     ADWRFEDTDGRVRHDWQAADELARMPILRLGPGETLYEVIALESSFGILTDPGSIRAW
                     CRVKDEVSMPIQLSRVVAPPTTPPSVLRDAGPLLGEKARETIQVKLWSACAGDGDAWF
                     DCDEALYTVAWDRMQGAPDEAEAIVDTLIARHADSGWCRPAIHDLLSRLPEAAGRRWL
                     EGVIARAPGGVAENYAREMMRRTGYGQFPAAR"
     gene            2611..3366
                     /locus_tag="VF720_07490"
     CDS             2611..3366
                     /locus_tag="VF720_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7879238.1"
                     /translation="MNAPFEGQVALVTGGARGIGRATAMKLAAGGADLAILYLNSHDE
                     ADQVCAEARGMGRRAIAIQADVADAASLAEAFETLKGTFDRVDIVVSNAALGVLKPAL
                     EMTTRHWRRCMETNALALNLLAQLAVPMMPRGGRFVALSSLGSTRAIPDYAFIGASKA
                     ALESLARSLAQELGPRGIRVNIVNAGVVDTDALSAFPRREELLAEQARRAPLGPVLTP
                     DDVAGAVYLLCRPEAAMITGHVLVVDGGFSISG"
     gene            3363..4127
                     /locus_tag="VF720_07495"
     CDS             3363..4127
                     /locus_tag="VF720_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017529204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7879239.1"
                     /translation="MTPFDPADAGLKGGTAIVTGGNRGIGRAIVETLAAAGMNVVFTW
                     RTGREAADEVLAAAGGGTIRGEALDVRDSEACAAFVERVAEANERVDLLVNNAGVIRD
                     KPLAAFEDEDVRDVIDTNVVGAFNMCRATVPWMVRQRSGTIINLSSVAATKGGRGQTN
                     YAASKGAIDALTRSLAVELAPRRIRVNAVAPGVIDTDMSREVLDLAGDQVQSRILMRR
                     VGRPDEVASVVWFLASKHADYITGQVLNVDGGFKME"
     gene            4133..4576
                     /locus_tag="VF720_07500"
     CDS             4133..4576
                     /locus_tag="VF720_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018577138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX7879240.1"
                     /translation="MTASTINPEEIAAIYPQVAAIMSSALGCDPEKVRPDAALIDDLG
                     AESIDFLDIVFRLEKTFKVKIPRGKIIADARGDLSESDFEKGGIVTEKGMECLRSYLS
                     EVPAERIKTPLKVAEIPRLFTVETFCKIVLRQQRAMSEAGGSSPA"
     gene            4598..5440
                     /locus_tag="VF720_07505"
     CDS             4598..5440
                     /locus_tag="VF720_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879241.1"
                     /translation="MNRRYRAFSFVDRIDELTIGRSAAGRYAVSDTIDLFPTSLVAEA
                     VGQLAAWSVMAATDFRLRPVAGIAGRIEFGDPPRPGDILTLSAVIDALDDDSVTYHGE
                     ARCEGRLLVRLFDCIGPMLPMQDFDEPADVRRQFERVRGEGAEPFGFAGFPDLPLTPL
                     PGESGNSAGTEFTVPATLDLFGDHFPRRPVLPGTILMHQNLVLATAIAARIPTLVHGT
                     AWSLVALSEVKIRAFTPPGERIELAGQADDSTGTELSVAIESRMKGRMIAGSRAEFKA
                     MAAR"
     gene            5437..6738
                     /locus_tag="VF720_07510"
     CDS             5437..6738
                     /locus_tag="VF720_07510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006872266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEX7879242.1"
                     /translation="MNRARRIAVTGIGLVTPLGNERASTWEGLLAGRSGCAEIEGFDA
                     SGFPTRIGAEVKGFEVNERLGDGRILKVASRAVRFALAAADEAFRDAGIRPTPDSADR
                     WGLAVGAGMMGVSFDELNEIHRWCAADGTFDPARLVAPDYPADSVAFVRSQTNAGLGV
                     LARRFGIHGPATAVHTACASGGQAIGSALKLLRRGEADAILAGGYDSMLNPLGLSAFC
                     LLGALSTDNDRPARASRPFDATRNGFVLGEGAGFLVLEEWSRAVARGARIYAELAGDG
                     NSLSCYRITDSAPDGDGAIQAMQQALGDAGVTAADIDYINAHGTSTLMNDRGECAAMT
                     RVLGPAVDRVLVSSTKSCMGHLIAAAGAVEAAVCCLAIHHGIAPVNANHESFDPECAV
                     NLVLGNSRPAPIRVALSNSFGFGGSNSSLVFRHPDAGKAAR"
     gene            6735..8027
                     /locus_tag="VF720_07515"
     CDS             6735..8027
                     /locus_tag="VF720_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX7879243.1"
                     /translation="MTKRPRAVITGLGAVCASGSSPEAIARAVREGRSGIGPLTRWDP
                     AGWAVKRAGEIREVDDRTLVPDRKLHKMVSRLDLLGIHAADRAIEASGLAAYRASLDP
                     ASALAFNDRSGILTGSGGGSYRFNDDFHPLLTEADGSMKRFGEEHASIVNPMWLLRIL
                     PNNVLCHVGITHQFKGTNACITNQCTGGILAAAEGAAALRSGESDRVLAVGYDSPVEP
                     ELVFHYDRLGLASSDELRPFDAGRTGTILGEGSAAIVLEHADAADRRGAPILGEILGS
                     GSVTEGSGIVEIRPDGDGVFRAMSRALLDAGLEPADIGMIVAHANGTPLSDATEAMAI
                     RRLFGNDPPPVTAFKWSYGHTLAACGMLDLVTTIHSLRAGVVPGIPTLRTLDPALAPF
                     PVSASGRPPRNDTALVICRGFGGMNAAIVVRAPLTPGE"
     gene            8030..9238
                     /locus_tag="VF720_07520"
     CDS             8030..9238
                     /locus_tag="VF720_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEX7879244.1"
                     /translation="MAPPPGARAGLDTVPLDRMARLLRQSDADLHRLFDAEEFRDAGN
                     GPGRIGSLAARFAAKEACCKLFPRETALGTIEPMDFAVRNDPFGAPSIEPSPTARTVL
                     DRHRVERIRVSLSHTAEVATAIAWTEPKAIDPPWYGRLIYNVLPLRRKIVLANLRRVF
                     GDDLAEPEIRRLAQAFYGHYVRCFAELLGRPFLSSEKRKSWVRIENLESPVEAHKQGK
                     GIILLTGHFGNWEMATVAGIGQLSQYKGLLHFVRRPLKPEILDRFVTARFRNAGFGVL
                     GRRDSLEEILDLLSGGAIVVIVFDQHAGGRRGIPVDFLGSPADTFKSLAILAMSTGAP
                     VIPASSWREPDGSHVLRFEEPLQPIEDPDVGAAIRLNTRSYNEALERMLLRHPEQWIW
                     MHKRWKIDGR"
BASE COUNT         1419 a         3261 c         3187 g         1427 t
ORIGIN      
        1 cgactcgagc aggtgttcct cgaggccggg cgccgaaaaa tcccagtcga cgacgtcgtg
       61 ccagtcggcc acccgccggg tcggacgccc gcgggcgtca cgctggaacc agtcagggtg
      121 gctggccgcc tccacgtggt cgggcgcgcc atggttgaac acgcagtcga ggatcaactt
      181 gaggccggcg tcgtgcgcgg cgcggacgag cgccaggaga tcgtccttgc ggccgtattc
      241 gggattgatg gcgcggtaat cgcggatggc gtacggcgag ccgaggcgcc ctttccgggc
      301 cgattcgccg atcggatgaa tggggaggag ccagaggata tcggccccca gctcggcgat
      361 ggccccgacg cgatcggcca caccgcgcag cgttccctcc ggggagaacg ccctcgggta
      421 ggccgagtag atgacggcgt tgcgggccca ggcgggaggc tccggggagg cgcctcgtgg
      481 cgtgccgggc ggttcacgcc ggacgccgtt ccggcttccg acctcgggtg gcggaaccgg
      541 cagaggccgc tcgcggttga cagggcgccg cttcatcccg tttcctcgtc ctcccctcgc
      601 gacaagccgt aacgcccgga gtgcccggcc acacgatcat gacgcgcgca ttgtacacag
      661 ccgattcgga cgtgcgatga gacgcctcgt cctcatcttc atcgacggcg tgggggtcgg
      721 ccgacccgat ccggacgtga atccgctggt cgggctcccg atcctcggga acacacttcc
      781 ccaggactgg cacccgccgc tcgatggcgg tcggccggcg tcgctccccg acctggtgcg
      841 cgaggcgccg cttccgaatg gcggcgtggc ccgcgccacc gacccctcgc tcgggatgcg
      901 cggccttccg cagagcgcca ccgggcagac caccctcttc acgggggtga acgcggccga
      961 gcgcctcggg catcacctgt acggctatcc cggcccaacg ctccggaaga tcctcctcga
     1021 gagctcggtg ctgaagagag ccaccgaagc gggctaccgg gcggccttcc tcaacgccta
     1081 tcgacccctc ttcttcgatc tgggcgagga gatctggaat cggccgatga gcgcctcctc
     1141 gtggaacaac cgggcggcgg ggctcccctt catgaccgtg gacgacatcc gtgatcggcg
     1201 ggccctttat cacgattaca ccaatcgcga gccgatcgag cgggggatcg aacttcccga
     1261 atggtcgccg gaggaggcgg gccgggtgct cgccgaggcg gcggcggagc acgatctcgc
     1321 gatttacgag tatttcctca gcgaccgggt cgggcatact ggcgagcatg cacgggcccg
     1381 cacgattgcc gaagggttgg atcgcttcat cgggtccgcc gtgggagccg ccgacctgtc
     1441 ggagcagacc atcgtggtga cgagcgatca cggcaacatc gacgacatga cctcgcgctc
     1501 gcacacgatg catccggtgc cggcgctcgt gtggggcccc gacgcgcccc ggctcgccgc
     1561 ccgcctcgac aggctcgagg actttgcggg agccatgctc gatcacctcg gaagccgcgc
     1621 gtgagacgag cggccccgcc gatggacgtc cgcgtggtga ccgcgttcct tggtgccgcg
     1681 ctgctccccg ccctctccgg ctgcaccgca accgggccca tggccgcgag cagcgggaaa
     1741 tcggggtcga tcctggcttc gcgccaggcg ggcgacgtgc cgcccgcgcc ggccagcccg
     1801 atcgtggtca cgctgcacgc gcttcgcgac acggtggtgg cgcaccagac cgcctgggtg
     1861 gaggtgcgca tcaccaacaa cggacccggc ggcctggaca tccccgaccg gggacagcac
     1921 ctggtggccg actggcgctt cgaagacacc gacggccgcg tgcgccacga ctggcaggcc
     1981 gcggatgagc tggccaggat gccgatcctc cgtctcggcc ccggcgaaac gctctacgag
     2041 gtgatcgcgc tggagagcag cttcggcatc ctcaccgatc ccggctcgat ccgcgcctgg
     2101 tgccgcgtga aggacgaggt gtcgatgccg atccagttga gccgcgtggt cgcgcccccc
     2161 acgacaccgc cgtcggtcct gcgcgacgcc gggccccttc ttggcgagaa ggcccgcgag
     2221 acgatccagg tgaagctctg gtcggcctgc gccggcgacg gcgacgcctg gttcgattgc
     2281 gacgaggccc tctacaccgt ggcatgggac cggatgcagg gagcacccga cgaggccgag
     2341 gccatcgtcg atacgctgat cgcgcgccac gccgacagcg gctggtgccg gccggccatc
     2401 cacgatctcc tctcgcgact ccccgaggcc gccggccgcc gttggctgga gggcgtcatc
     2461 gcccgggcgc cgggcggcgt ggccgagaac tacgcccgcg agatgatgcg cagaaccggt
     2521 tacggccagt tcccggccgc acggtaggtc cgcctggagg cgaaaggggc ctgcttgccc
     2581 tgatccaccc cctttgtgga tgatgccggc atgaacgcac ccttcgaggg acaggtggcc
     2641 ctggtcacgg gcggtgctcg tggcattggc cgggccacgg ccatgaagct ggcagccggg
     2701 ggcgccgacc tggcgatcct ctatctgaac agccacgatg aggcggacca ggtctgcgcc
     2761 gaggccaggg gcatggggcg gcgcgccatc gccatccagg ccgacgtggc cgacgcggcg
     2821 agcctggccg aggcgttcga aactctgaag ggcaccttcg accgggtcga catcgtggtg
     2881 agcaacgccg cgctgggcgt cctgaagccc gcgctcgaga tgacgaccag gcactggcgc
     2941 cgctgcatgg aaacgaacgc gctggcgctc aatctcctgg cgcaactggc cgtgcccatg
     3001 atgccccggg gcgggcggtt cgtggccctg tcgagcctcg gctcgacccg ggcgatcccg
     3061 gactacgctt tcatcggcgc ctcgaaggcg gccctcgaat cgctggcccg gagcctggcc
     3121 caggagctcg ggcccagggg aatccgggtc aacatcgtca atgccggcgt cgtcgatacc
     3181 gatgccctct ccgccttccc caggcgcgag gaactcctgg ccgagcaggc gcgccgcgcc
     3241 ccgctgggcc ccgtcctgac gccggacgac gtggcgggag ccgtctactt gctctgccgc
     3301 cccgaagccg ccatgatcac cggccacgtt ctggtggtgg acggtggctt ctcgatctcc
     3361 ggatgacacc tttcgatccg gcggatgccg gcctcaaggg tggtacggcc atcgtcaccg
     3421 gcggcaatcg tgggatcggt cgcgccatcg tggaaacgct ggcggcagcg gggatgaacg
     3481 tggtcttcac ctggcgaacg ggccgggagg cggccgatga ggtcctggcg gcggccggcg
     3541 gcgggacgat ccggggcgag gcgctcgacg tccgcgattc ggaggcctgc gccgcgttcg
     3601 tggagcgggt ggccgaggcg aacgaacggg tcgacctcct cgtcaacaat gccggcgtca
     3661 tccgcgacaa gccgctggcg gcgttcgagg acgaggatgt ccgggacgtg atcgacacga
     3721 acgtcgtcgg cgccttcaac atgtgccggg ccacggtccc gtggatggtc cggcagcgca
     3781 gcgggacgat catcaacctg agctcggtcg ccgccacgaa ggggggccgg ggacagacga
     3841 actacgcggc cagcaagggg gcgatcgacg ccctcacgcg gtcgctggca gtggaactgg
     3901 caccgcgccg gatccgcgtc aacgcggtgg cgccgggcgt catcgacacc gacatgagcc
     3961 gcgaggtgtt ggatctcgcg ggtgaccagg ttcaatcacg gatcctgatg cgccgtgtcg
     4021 gccgcccgga cgaggtggcc agcgtcgtct ggttcttggc ctcaaagcac gcggactaca
     4081 tcaccggcca ggtgctgaat gtcgacggcg gcttcaagat ggagtgaaca gagtgacggc
     4141 atcgaccatc aatcccgaag aaattgcggc catctatccc caggtggccg ccatcatgtc
     4201 gagcgcgctt ggctgtgatc ccgagaaagt ccgccccgac gcggccctga tcgatgatct
     4261 cggcgccgag tcgatcgact tcctcgacat cgtcttccgc ctcgaaaaga cgttcaaggt
     4321 gaagatcccg cgggggaaga tcattgccga cgcccgcggc gacctctccg aatcggactt
     4381 cgagaagggg ggcatcgtga ccgagaaggg gatggagtgc ctcaggtcgt acctctccga
     4441 ggttcccgcc gagcgcatca agaccccgct caaggtggcc gaaatcccgc gactcttcac
     4501 ggtcgagacg ttctgcaaga tcgtcctccg ccagcagcgc gccatgtccg aggccggcgg
     4561 ttcgagcccg gcctgaccgc cgtgcccgcc gcccgcgatg aatcgtcgct accgtgcttt
     4621 ctctttcgtc gaccggatcg atgagctgac gatcggtcgg tcggctgccg gccgctatgc
     4681 ggtttccgac acgatcgacc tgttcccgac ttcgctggtg gccgaggcgg tcggccagct
     4741 ggccgcctgg tcggtaatgg cggccaccga cttccggctc cgtccggtgg ccggtatcgc
     4801 cggccgcatc gagttcggcg atccgccgcg tccgggggac attctgacgc tgtcggccgt
     4861 gatcgacgcc ctggatgacg actcggtgac ctatcacgga gaggcgcggt gcgagggaag
     4921 gctcctggtc cgtctgttcg attgcatcgg cccgatgctc ccgatgcagg atttcgatga
     4981 gccggccgat gttcggcgcc aattcgagcg cgtgcgcggg gagggcgccg agccgttcgg
     5041 tttcgcgggg ttcccggacc tgccgctcac cccgttgccc ggcgagagcg ggaattccgc
     5101 gggcacggaa ttcaccgtgc ccgccacgct cgacctgttc ggcgatcact tcccgcgccg
     5161 accggtcctt cccggaacga tcctgatgca ccagaacctc gtgctggcca cggcgatcgc
     5221 cgcgcggata ccgacactcg tacacggtac ggcctggtcc ctcgtggcac tgagcgaggt
     5281 caagatccgg gccttcacgc ccccgggcga gcggatcgag ctggccgggc aggccgacga
     5341 ttcgacgggc acggaactgt ccgtcgcaat cgagtcgcgg atgaaggggc ggatgatcgc
     5401 cggcagccgc gccgagttca aggcgatggc ggcgcgatga accgcgcacg gcggatcgcc
     5461 gtcaccggga tcggcctcgt gacgccgctc ggcaacgaac gggcctcgac ctgggaagga
     5521 ttgctggccg ggcggagcgg gtgcgcggaa atcgaagggt tcgacgcgtc aggcttcccc
     5581 acgcggatcg gcgccgaggt gaaggggttc gaggtcaacg aacgcctcgg cgacgggcgg
     5641 atcctcaagg tggcgtcgcg tgcggtccga tttgcgcttg ccgccgcgga cgaagcgttc
     5701 cgggacgccg gcatccgccc tacgccggac tcggccgatc gctgggggct ggccgtcggc
     5761 gcgggaatga tgggtgtttc cttcgacgag ctgaacgaga ttcatcgctg gtgtgccgct
     5821 gacggaacgt tcgatccggc caggctcgtc gctcccgact atccggcaga ctcggtggcc
     5881 ttcgtccgca gccagaccaa tgcggggctc ggtgtcctgg cccgccggtt cggcatccac
     5941 ggcccggcca ccgcggtcca taccgcctgc gcctccggag gacaagcgat cgggagcgcg
     6001 ctgaagctcc tccgccgcgg cgaggccgat gccatcctgg ccggcggcta cgactcgatg
     6061 ctcaatcccc ttggcctgtc ggccttctgc ctcctcggcg ccctctccac cgacaacgac
     6121 cgtcccgcgc gcgccagccg gccgttcgat gccacccgca acgggttcgt cctcggcgag
     6181 ggagccggct tcctcgtcct cgaggagtgg tcccgcgccg tggcccgcgg cgcccgcatc
     6241 tacgccgaac ttgccggcga cggcaactcg ctctcctgct accggatcac cgactcggcc
     6301 cccgacggcg acggcgccat ccaggccatg cagcaggcgc tgggcgatgc cggtgtgacc
     6361 gcggccgaca tcgactacat caacgcccac ggcacctcca cgctcatgaa cgaccggggg
     6421 gaatgcgcgg cgatgacgcg cgtcctcgga ccggctgtcg atcgcgtcct cgtcagctcg
     6481 accaagagtt gcatggggca cctgatcgcg gcggccggcg cggtcgaggc ggctgtctgt
     6541 tgcctggcca ttcatcacgg catcgcgccg gtcaacgcca accacgaatc gttcgatccg
     6601 gagtgcgccg tcaatctcgt gctcggcaac tcgcgaccgg cgccgatccg cgtggcgctc
     6661 tccaattcct tcggcttcgg cgggtcgaac agcagcctcg tcttccgtca tcccgatgcc
     6721 ggaaaggcgg cgcgatgacg aagcgtccgc gcgcggtcat caccgggctc ggcgccgtct
     6781 gcgccagcgg gagcagtccc gaggccattg cgcgggcggt ccgggaaggt cggtcgggga
     6841 tcgggccgct cacccggtgg gacccggccg gctgggcggt aaagcgagcc ggcgagatcc
     6901 gggaggtcga cgaccggacg ctcgtgccgg accggaagct ccacaagatg gtctcccggc
     6961 tcgacctcct cggcatccat gccgccgacc gggccatcga ggcttcgggg ctggcggcgt
     7021 accgggcgtc actcgacccg gcatcggctt tggccttcaa tgaccgaagc gggatcctga
     7081 ctggatcggg cggcgggagc taccgtttca acgacgactt ccatcccctc ctgaccgagg
     7141 cggacggcag catgaagcgc ttcggcgagg aacacgcctc gatcgtcaac ccgatgtggc
     7201 tgctgcgcat cctccccaac aacgtcctct gccacgtggg catcacccat cagttcaagg
     7261 ggacgaacgc ctgcatcacc aaccagtgca cgggtggaat cctggcggcc gccgagggag
     7321 ccgcggccct ccggagcggc gagtcggatc gggtcttggc cgtcggctac gactcgccgg
     7381 tcgagccgga actcgtcttc cactacgacc gcctcggcct cgcctcgtcc gacgaactcc
     7441 gcccgttcga tgccggccgc accggcacga tcctcggcga ggggagcgcc gccatcgtcc
     7501 tcgagcatgc ggatgcggcg gatcgacggg gcgccccgat ccttggagag atcctcggct
     7561 ccggcagcgt taccgagggg agcggcatcg tggagatccg gccggacggt gatggcgtct
     7621 tccgggccat gtcgcgcgcg ctcctggatg cggggctcga gcccgccgac atcggcatga
     7681 tcgtggccca cgccaacggc acgccactct ccgacgccac cgaggccatg gcaatccgcc
     7741 gcctcttcgg taacgacccg ccgccggtca ccgccttcaa gtggtcgtac ggacacacgc
     7801 tggctgcctg cggcatgctc gatctcgtga cgacgatcca ttcgctccgc gcgggcgtcg
     7861 tgccgggaat cccgacgctg cgcaccctcg atccagcact ggccccgttc ccggtgtcgg
     7921 catccgggcg gccgccccgc aacgacaccg ctctcgtcat ctgccggggc ttcggcggta
     7981 tgaacgcggc catcgtcgtg cgcgccccgc tcacgcccgg cgaatgaacg tggcgccgcc
     8041 cccgggagcc cgggccggcc tcgacaccgt tccactcgat cgaatggcac gactcctccg
     8101 ccagtccgac gccgatctcc accggctttt cgacgcagag gagtttcgcg acgccgggaa
     8161 cggccccggc cggatcggca gcctcgcggc ccggttcgcc gccaaggagg cgtgctgcaa
     8221 gctcttcccg cgtgagacgg cgctgggcac gatcgagccg atggacttcg cggtgcgaaa
     8281 cgatccgttc ggggcgccct cgatcgaacc gagcccgacg gcgcgcacgg tgctcgaccg
     8341 tcaccgggtg gagcggatcc gggtgtcgct gagccacacc gccgaagtgg ccaccgccat
     8401 cgcctggacg gaaccgaaag cgatcgatcc cccctggtac ggccggttga tctacaacgt
     8461 cctgcctctc cggcggaaga tcgtgctcgc caacctccgt cgcgtcttcg gcgacgacct
     8521 ggcggaaccg gaaatccgca ggctggcgca ggccttctat ggccactacg ttcgctgctt
     8581 cgcggagctc ctggggcggc cgttcctgtc gtccgaaaaa cggaagtcgt gggtgcgcat
     8641 cgagaacctc gagtcgccgg tcgaggcgca caagcagggg aagggaatca tcctcctcac
     8701 cggccacttc gggaactggg agatggccac cgtggccggc atcggccagt tgagccagta
     8761 caagggactg ctccacttcg tccgccgccc cctcaagccg gaaatcctgg acaggttcgt
     8821 cacggcccgc ttccggaacg ccggattcgg tgttctcggc cggcgagact cgctcgagga
     8881 aatcctcgac ctgttgtccg gcggcgcgat cgtcgtgatc gtcttcgacc agcacgccgg
     8941 cggccggcgc ggcatcccgg tcgatttcct cggctccccg gccgacacgt tcaagagcct
     9001 ggccatcctg gccatgtcca ccggggcgcc ggtcatcccc gcctcgagct ggcgcgaacc
     9061 ggacggctcg catgtcctcc gcttcgagga gccgctgcag ccgatcgagg accccgacgt
     9121 cggcgccgcc atccgcttga acacgcggag ttacaacgaa gcgctcgagc ggatgctgct
     9181 gcgccatccc gaacagtgga tctggatgca caagcggtgg aagatcgacg ggcggtaggg
     9241 gcggcggtgc ggagcccgat caccggatca ccagcacctt ctgaacgctt cgca
//
LOCUS       DASVOD010000333         3055 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_441280, whole genome shotgun sequence.
ACCESSION   DASVOD010000333 DASVOD010000000
VERSION     DASVOD010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441280"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VF720_07525"
     CDS             <1..994
                     /locus_tag="VF720_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879245.1"
                     /translation="EPAGLFVASGGSGYIHAGSLLTWSVSTGGETAIIGFDKTGGQAP
                     PVAGVIVRVYDGASSLTADRAPDAVIHPWLTLPLRHLEIAEGGAANLSFQLGGSTLSP
                     GQFNGLLLFETNDQSAPEVQLPLALTIDNDVPVVFGTFTVVPLPEGLELNWTTSSEVD
                     HLGFRLVRRTIAPVGAGEVGIGPELIRPSGDGTSYRVIDTGTQPGFTYDYRLADVSRS
                     GRLTWHGPFRITAPGPTLPTALQLSPAVPNPAPGAASLAWALPATGPVELTLFSVDGR
                     AVRHLLRRAVHEAGWHATAWDGRDDSGSEVPSGVYFYRLEGAGTALSRKLLLVR"
     gene            1103..>3055
                     /locus_tag="VF720_07530"
     CDS             1103..>3055
                     /locus_tag="VF720_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7879246.1"
                     /translation="MTGARPQFRHAAAVVRRILMAGACLALAPAVTLPAVAAGADRIP
                     TVRTVFLRYTFDSRSPIGAWESTVPMRKTLAAPGYTTIGPLHVEEVEPTDSGGTRVTS
                     RLTGRPLIRASTIWNGLGRLFEPDPGAFFPIRTSDRPVAAPRAISIRENQYTSRIAAE
                     RIWEAARKTAPIERLAAVSNYEVVIWAHFYGVGLPDPGSAEWWIVATSRPEAPRDIGV
                     TSVDWPTGVTPTGACLAPEVTVTNFGDGPASFRVALEVAAPGISDHHAVQTVENLPGD
                     GSRRLRFEPLTTGQAGSGSVSVDILPVNGEAWSDAFADNDLAHREITVVAGGVFRGRP
                     AVYDPESPTDIPPSHSMPVDVDSDGNFELFQLGYRSRLWRSLPTRQWEEITSSAIAAL
                     APWPRYVLTRDWDADGRADILVVYWDAAPVLLRGDGAGGFIDGTSASGLDGAINKAVV
                     ADIDLDGDGHRDLIVADTGRKKFLRNDGLGHFTDVSLGAVVFDESKTQSIAVADFDAD
                     GDEDFYLSNWDGPGRLYRNDTARFTLLLTLDGLERWGAGFVDLDGDARLDLVDLSTSA
                     TRAWRNRNNRQFDEITSTLGPLPRGFDVLGSDLDGDGTHEMVFSTLDGLKLFARDPNG
                     GPAFDDRSNLLVGQKGFGGSNMPILVL"
BASE COUNT          470 a         1062 c         1001 g          522 t
ORIGIN      
        1 cgagccggcc ggcctcttcg tggcttccgg cgggtcgggc tacatccatg ccggatcgct
       61 tctcacctgg tcggtatcga ccggcggcga gacggcgatc atcggcttcg acaagacggg
      121 gggccaggcg ccacccgtag ccggggtgat cgtgcgtgtg tacgacggcg cgtcgtccct
      181 caccgccgat cgcgcaccgg acgccgtgat ccacccgtgg ctcacgctcc ccctgcgtca
      241 tctcgagatc gccgagggag gcgccgccaa tctctccttc cagctcgggg gatcgaccct
      301 gtcgccgggc cagttcaacg gcctcctcct cttcgagacc aacgaccagt cggccccgga
      361 ggttcaactt cccctcgcgc tgaccatcga caacgacgtg cccgtcgtct tcggcacatt
      421 caccgtcgtg ccgctgcccg agggactgga gctcaactgg acgacgtcga gcgaggtcga
      481 ccacctcggc ttccggctgg tgcgccgcac gatcgcgccg gtcggcgcgg gggaagtcgg
      541 catcgggccg gaattgattc gcccgtcggg cgacggaacg agctatcgcg tgatcgacac
      601 cggaacacag cccggcttca cctacgacta ccgcctggcc gacgtttcgc gaagcggccg
      661 cctcacctgg cacgggccgt tccgtatcac ggcgcccggg ccgacgctgc cgaccgcgct
      721 ccagctctcc cccgccgttc ccaatccggc acccggcgcc gcgtcgctgg cctgggcact
      781 gccggccacc ggcccggtcg agctcacgct cttctcggtg gatggccgcg cggtgcgtca
      841 cctgctgcgg cgggcagttc acgaggcggg gtggcatgca acggcgtggg atggtcgcga
      901 tgactccgga tccgaagtcc cgtcgggtgt gtacttctac cgcctcgagg gtgccgggac
      961 ggcattgagt cggaagctgc tcctggtccg gtaggcggtc cggctacagg ccgggcaatt
     1021 gtcgactaca attgtttcgg aaggcgcggc atccggcatc cgtcgggtga cgcgtctttc
     1081 ccctgccgcg gggagtcgaa acatgaccgg agcgcgtccc caattccgcc acgccgccgc
     1141 cgtcgtccgc cgaatcctca tggccggggc gtgccttgcc ctggcccctg cggtcacctt
     1201 gccggccgtc gcggcggggg ccgaccgcat cccgacagtg cgtaccgtct tcctgaggta
     1261 caccttcgat tcacgcagcc cgatcggcgc ctgggaatcg acggtcccga tgcggaagac
     1321 gctggccgcg cccggctaca cgacgatcgg gccgttgcac gtggaggaag tcgagccgac
     1381 cgactccggc gggacccggg tcacatcgcg actgacgggc cggccactca tccgggcctc
     1441 cacgatctgg aacggactgg ggcggctgtt cgagccggat cccggggcgt ttttcccgat
     1501 ccgcacgagc gaccggccgg tggccgctcc ccgcgcgatc tccatccgcg agaaccaata
     1561 caccagtcga atcgccgccg agcgcatctg ggaagctgcc cgcaagaccg caccgatcga
     1621 gcgacttgcg gcggtgagca actacgaggt ggtgatctgg gctcacttct atggggtggg
     1681 tctaccggat ccaggcagcg ccgagtggtg gatcgtcgcc acttcgcgcc ccgaggcgcc
     1741 acgggatatc ggcgtcacgt ccgtcgactg gcccacgggc gtcaccccga ccggggcctg
     1801 cctcgcaccc gaggtaacgg tcaccaactt cggggatgga ccggcgtcgt tccgtgttgc
     1861 gctcgaggtg gcggcgcctg ggatcagcga tcaccacgca gtacagacag tcgaaaacct
     1921 tccgggcgac ggctcccggc gcctccgctt cgaaccactg acaacgggcc aggcgggatc
     1981 cggttccgtt tcggtagaca tccttccagt caacggtgag gcctggtcgg atgccttcgc
     2041 ggacaacgat ctcgcccatc gcgagatcac tgtcgttgcc ggcggtgtct tccgcggtcg
     2101 cccggcggta tacgacccgg aatcgcccac cgatattccg ccgagccaca gcatgccggt
     2161 cgatgtcgat tcggacggga acttcgagtt gttccagctg ggatatcgct cccgcctctg
     2221 gcggtcattg ccgacgcgac agtgggaaga gatcacgtca tcggcgattg ccgcccttgc
     2281 accgtggccc cgctacgtcc tgacgcgcga ctgggatgcc gacggccgtg ccgacatcct
     2341 ggtcgtctac tgggacgcgg cgccggttct gctccgtggc gatggcgccg gcgggttcat
     2401 cgacgggacg agcgcctccg gcctcgacgg ggcgatcaac aaagccgtcg tggcggacat
     2461 cgacctggac ggggatggcc accgtgacct gatcgtcgcc gacacggggc gcaagaaatt
     2521 cctccggaac gacgggctcg gtcacttcac cgacgtcagc ctgggcgcgg tggtgttcga
     2581 cgaatcgaag acacaatcga tcgcagtggc ggacttcgac gctgatggcg atgaggattt
     2641 ctacctgtcg aactgggacg gtccggggcg actctatcga aatgacaccg cccgcttcac
     2701 gctcctgctc acgctggacg gcctcgaaag gtggggcgcc ggcttcgtcg acctggatgg
     2761 cgatgcccga ctcgacctcg tggatctctc gacatccgcc acgcgtgcct ggcgaaaccg
     2821 gaacaaccgc cagttcgatg agatcacctc gacgctcggg ccccttccac ggggcttcga
     2881 cgtattgggc agcgacctgg atggcgatgg tactcacgaa atggtcttct cgacactcga
     2941 cggcttgaaa ctcttcgctc gcgacccgaa cggtgggccg gccttcgacg atcgctccaa
     3001 cctgctcgtc ggccagaagg gcttcggtgg ttcgaacatg ccgatcctgg tgttg
//
LOCUS       DASVOD010000334         4192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_493405, whole genome shotgun sequence.
ACCESSION   DASVOD010000334 DASVOD010000000
VERSION     DASVOD010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493405"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VF720_07535"
     CDS             complement(<1..561)
                     /locus_tag="VF720_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879247.1"
                     /translation="MTILTLRTRAFPTGGLLAAGLVSCLVAMAQPAAGAATWRADFTT
                     GAGYDDDVLGRPGEDSSFPVLATGYLQLAPSIRGSLSSGAWRLAGGWDYVMNAYEQRD
                     AGLYHDNRATLGAAYAPTRSVRFRLDGEAEWFHRTEFQDYDFDRLEGTPSVAWFVADD
                     WRLDATWRHGRTTYPERTATPLTLDEQ"
     gene            complement(574..1164)
                     /locus_tag="VF720_07540"
     CDS             complement(574..1164)
                     /locus_tag="VF720_07540"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HEX7879248.1"
                     /translation="MTDERIENERELNPGDRARLDALSRASLEGRGTGAPLDFAARVM
                     ARVEAERAVTPKWSTGLAAFWRRPAVRWASGLAAAALALWVASAALRKPVPETPTLKA
                     APLSAESSTTVPHRFTIEAPEARRVCLVGDFNDWAVCEVPLVRDETAGTWSVQMELPP
                     GRHEYMFVVDEGWVTDPSAPVSVDDGFGNRNAVIFL"
     gene            complement(1199..1813)
                     /locus_tag="VF720_07545"
     CDS             complement(1199..1813)
                     /locus_tag="VF720_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7879249.1"
                     /translation="MGVPKVVTEPTSGGFIDSVEHDDRLVEMARGGDESAYRRLVERH
                     QDRIVGVTLRLLKNRDEAIEAAQDVFVRAWRSLGRFEKRSRVSTWLHRIALNVCYDRL
                     GVRKRKNEVPLDDLLAGGFEPGDEAGGATDDSVIDAQSVREFEEAVAALDETYRVIFV
                     LRQVEGRPYEEIAGALGISVGNAKVRVHRAREMIVTALKRRGVL"
     gene            2006..3127
                     /locus_tag="VF720_07550"
     CDS             2006..3127
                     /locus_tag="VF720_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEX7879250.1"
                     /translation="MKARNTLATLAAAVALLISAGGCGDDSNTSVTPDGYGQVTVKVT
                     DAPKVDLTDISNLFVTFDRLVILTQADSLPDDSTDTPDRIEVLTSPITFDLLSLTNGL
                     SQALGQADLPAGNYRGLVVDIAPDGAWLIESDGDTFDVKVPSGRLFVRTDFTVDEDQN
                     TEVLLDFDVAASLHLIQTGNGKYILRPVLRQLPPAPEAGSVAGQVLVQTDNGLVPAGE
                     ILVPNPWRGHPAQDHGNGRRPPGHGNQGRRPDDRAMVPLPVAFPMVIQAHVHGDSTDD
                     DEEDDAIRARLTTLGDDDEEDGEDHDDNPHRPHGRGTLVDKDGDYLIVRLRKDVEYDL
                     GLKIHPRAGFEVVSGPGTLLVDGDETGQNFILRLKPETP"
     gene            3187..>4192
                     /locus_tag="VF720_07555"
     CDS             3187..>4192
                     /locus_tag="VF720_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEX7879251.1"
                     /translation="MTLSLQKSDQALIAPYVTNTDGSVYALRHLPEEVVAVLFAYYSR
                     SRESLRDNLLRLIKEGDLALATTGPDVAHAPADDDFISRAADKARAFHEKWVVGYGHS
                     SVAEHAVVHLAIEDVSILASKAIEDARLASYTEKSTRYVPFDTARHHVPESFRNDGAS
                     GQAWRRITSALSRAYDDSMPLLVDWFVANRAPKPGQTDKGHVAACRAQACDVLRYLLP
                     AATHTNLGFTANARVLESLLARLLSHPLAEVREIGARMKAEALLVVPTLIKYAEPSDY
                     RRATHEAMRDRAAEILGSAADPAPTESVRLIRWDTDAEERIVEAILSGASDRSGETIR
                     N"
BASE COUNT          691 a         1531 c         1313 g          657 t
ORIGIN      
        1 ctgctcgtcc agggtcaacg gcgtggccgt gcgctcggga taggtcgtgc gcccgtgccg
       61 ccaggtggcg tccagccgcc agtcatccgc cacgaaccag gccacggaag gcgtcccctc
      121 gagccggtcg aagtcgtaat cctggaattc cgtgcgatga aaccactcgg cctccccgtc
      181 gagccggaac cgcaccgatc gcgtcggcgc gtaagcggcg cccagcgtgg cgcggttgtc
      241 gtggtagagg cccgcgtcgc gctgctcgta cgcattcatc acgtaatccc agcccccggc
      301 gagccgccag gcgcccgacg agaggcttcc ccggatcgac ggcgccagct ggagatatcc
      361 cgtggccagc accggaaacg agctgtcctc gcccggccgc cccagcacgt cgtcgtcgta
      421 gccggccccg gtggtgaagt cggcgcgcca cgtcgcggcg cccgcggccg gctgcgccat
      481 ggccacgagg cacgaaacga gtccggcggc caggaggccg ccggtcggaa aggcgcgcgt
      541 gcgcaaggtc aggatcgtca tcctgaccgg agcttacagg aagatcaccg cgttccggtt
      601 gccgaagccg tcatccaccg agaccggggc cgaggggtcg gtgacccacc cttcgtccac
      661 cacgaacatg tactcgtggc ggcccggcgg gagctccatc tgcacgctcc aggtgccggc
      721 cgtttcgtcg cgaaccagcg gcacctcgca gacggcccag tcgttgaaat cgcccaccag
      781 gcagacgcgc cgcgcctcgg gggcttcgat cgtgaagcgg tggggaacgg tcgtgctcga
      841 ttcggccgag aggggcgcgg ccttcagcgt gggcgtttcg ggcaccggct tgcgcagcgc
      901 cgcggacgcc acccacaacg ccagcgccgc ggcggcgagg cccgaggccc aacggacggc
      961 gggacggcgc cagaaagcgg cgaggcccgt ggaccacttc ggcgtgacgg cccgttccgc
     1021 ctcgacccgc gccatcaccc gggcggcgaa atcgagcggg gcgccggtgc cacgtccctc
     1081 gagggaggca cgcgacaggg catcgagacg cgcgcgatcg cccgggttca gctcccgttc
     1141 gttttcgatc cgctcgtccg tcattctctc ctcgcttcct tgatcaccct gtctttttct
     1201 acaggacgcc gcgccgcttg agcgccgtca cgatcatctc acgcgcccgg tgcacccgca
     1261 ccttggcatt tccgacgctg attcccagcg ccccggcaat ttcctcgtag ggccttcctt
     1321 ccacctgccg gaggacgaag atgacccggt acgtctcgtc gagcgccgca accgcttctt
     1381 cgaactcgcg gaccgactgg gcgtcgatga cgctgtcgtc ggtggcgcct cccgcctcgt
     1441 cccccggctc gaacccgcca gccaggagat cgtccagggg aacttcgttc ttccgcttcc
     1501 ggacaccgag ccggtcgtaa cagacattca gtgcgatccg atgcaaccag gtcgacaccc
     1561 gcgagcgctt ctcgaatcgc cccagggacc gccatgcccg cacgaacaca tcctgggccg
     1621 cctcgatcgc ctcgtcccgg ttcttgagca gacgaagcgt gacgccaact atccggtcct
     1681 ggtggcgctc aacgagccgc cggtaggccg attcgtcgcc gccccgcgcc atttccacca
     1741 gtcggtcgtc gtgctcgacg gagtcgatga accccccgga cgtcggttcg gtgaccacct
     1801 tcggaacgcc cacgaagccc tcgaactgcc gggcgtgacc ccggcctcgc tccttggaca
     1861 ggtgggggat accccggtta caggattggg gcgatccggc cgacaatgaa tcgtgattgt
     1921 ggttgtaacc cgggcggtag caaccggtcc aacccgtggc aagtcacact ataacctgtt
     1981 gccgcactgc aagaaggagg catcgatgaa agcccggaac accctggcga cgctggccgc
     2041 agcggtcgcc ctcctgattt ccgccggcgg ctgcggcgac gactcgaaca cgagcgtcac
     2101 gccagacggc tacggccagg tcaccgtcaa ggtgacggac gccccgaagg tcgacctgac
     2161 cgacatctcc aacctgttcg tcacgttcga ccgcctggtg attctcaccc aggccgacag
     2221 cctgcccgac gactcgaccg atacgcccga ccgcatcgag gtcctcacct cgcccattac
     2281 gttcgacctg ctcagcctca ccaacggcct ctcccaggcg ctcggccagg ccgacctgcc
     2341 ggcggggaac taccgcgggc tcgtcgtcga catcgcgccc gatggcgcct ggctcatcga
     2401 atccgacggc gacacgttcg atgtgaaggt cccctcgggc cgcctcttcg tgcgcaccga
     2461 cttcacggtc gatgaagacc agaacaccga ggtccttctc gacttcgacg tggccgcctc
     2521 gctccatctc atccagaccg gcaacggcaa gtacatcctc cgcccggttc tccgccagct
     2581 tccgccggct cccgaggcgg gctcggtggc cggtcaggtc ctcgtccaga cggacaacgg
     2641 cctggtcccg gcgggtgaga tcctcgttcc gaacccctgg cggggccatc cggcccagga
     2701 tcatggcaat ggccgtcgcc ctccgggaca cggcaaccag ggtcgccgtc cggacgaccg
     2761 ggccatggtg cctctgccgg tggccttccc gatggtgatc caggcgcatg tccatggcga
     2821 ttcgaccgat gacgacgaag aggacgacgc tatccgtgcc cgtctcacca cccttggtga
     2881 cgacgacgag gaagacggcg aggatcacga cgacaacccg caccgtcccc acggccgcgg
     2941 cacgctggtc gacaaggacg gcgactacct gatcgtgcgc ctccgcaagg atgtcgaata
     3001 cgacctgggt ctcaagatcc acccgcgggc cgggtttgaa gtggtgtcgg gccccggcac
     3061 cctcctcgtg gacggtgacg agaccggcca gaacttcatc ctccgcctga agcccgagac
     3121 gccctagtcg catccagttt tcgacgctac accttcatgc cggcgggacg atatgatccc
     3181 gccggcatga ctctttccct ccagaaaagc gaccaggcgc tcatcgcgcc ctacgttacc
     3241 aacaccgacg ggtccgtcta cgcgctgcgc catctccccg aagaggtggt ggcggtgctt
     3301 ttcgcctatt acagccggag ccgagagagc ctgcgcgaca acctccttcg cctgatcaag
     3361 gagggggacc tggcgctggc cacgacgggc cccgatgtgg cgcacgcgcc ggctgacgac
     3421 gacttcattt cgcgtgcggc cgacaaagcc cgggccttcc acgagaagtg ggtggtcggc
     3481 tacgggcact cctcggtggc cgagcatgct gtcgtccacc tggccatcga ggacgtgtcg
     3541 atcctggcca gcaaggccat tgaggatgca cggctggcgt cctacaccga gaaatccacc
     3601 cgctacgtgc cgttcgacac cgcccgccac catgtgccgg aatcgttccg caacgacggc
     3661 gcctcggggc aggcgtggcg ccgcatcacg agcgcgttgt ctcgtgccta cgatgactcg
     3721 atgccgctcc tggtcgactg gttcgtggcc aaccgcgcgc cgaaaccggg ccagaccgac
     3781 aagggtcacg tggcggcctg tcgcgcccag gcctgcgacg tgcttcgcta tctccttccg
     3841 gcggccactc acaccaacct tggctttacc gccaacgccc gcgtgctgga atcgctgctg
     3901 gctcgtctcc tctcccatcc cctggccgaa gttcgcgaga tcggcgcccg gatgaaagcg
     3961 gaggcgctgc tggtggtgcc cacgctcatc aagtacgccg agccctccga ttaccggcgc
     4021 gccacgcacg aagcgatgcg ggaccgcgcg gcggaaatcc tgggctccgc cgccgacccc
     4081 gcaccgaccg aaagcgtgcg gctcatccgc tgggataccg acgcggagga gcggatcgtc
     4141 gaagccatcc tgtcgggggc gtccgatcgg tcgggcgaaa cgatccggaa cg
//
LOCUS       DASVOD010000335         5282 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_648682, whole genome shotgun sequence.
ACCESSION   DASVOD010000335 DASVOD010000000
VERSION     DASVOD010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648682"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VF720_07560"
     CDS             complement(<1..198)
                     /locus_tag="VF720_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879252.1"
                     /translation="MIKRREILGVAALSAFLGAFVGLFLFGEFGGKVTPGPSATASPA
                     TGTVQTVDFQVGGDPGQPLTTT"
     gene            359..1621
                     /gene="murA"
                     /locus_tag="VF720_07565"
     CDS             359..1621
                     /gene="murA"
                     /locus_tag="VF720_07565"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015839002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX7879253.1"
                     /translation="MDKIVVRGGRSLRGTIEAAGSKNAALPILTAAILAPGEYRFTHV
                     PLLRDVTTMARLLETLGATFHQDGHTLHLDTSRVEPREAPYDLVKTMRASVYVLGPLL
                     ARFGEARVSLPGGCAWGPRPIDLHIKGMTALGAEISLEEGYVVARAEKLTGADIDLEI
                     ASVGATGNILMAAVAASGITRIRNAAREPEIPALAEFMNAMGARIEGAGTSEITIEGG
                     APLKPAPYRIIPDRIEVGTYLIAGAMTAGDVTLTGCRPDHVGALLMRLKQIGARVEAD
                     DDTIRVIGPERLDSFELRTDFYPGFPTDLQAQMMALGAVANGRSVLTDTVYTDRFTHV
                     AELQRLGADIQMERNVAVINGLKRLSGARVMATDLRASAALILAGLVAEGETHVSRVY
                     HIDRGYERIEERLTALGADVRRETEQGP"
     gene            1618..3549
                     /gene="selB"
                     /locus_tag="VF720_07570"
     CDS             1618..3549
                     /gene="selB"
                     /locus_tag="VF720_07570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEX7879254.1"
                     /translation="MTGRRFAVLGTAGHIDHGKTALVRLLTGIDTDRLKEEKERGISI
                     DLGFAHLSLPDGTECGLVDVPGHERFVKNMLAGVGGIDAVLFVIAADEGVMPQTREHM
                     DILRLLDVRRGVVALTKSDMVDADWLDFVTEEVRDWLAKGPLAGAPIVPVSSKTGAGR
                     EAVLEALVAALAELPERSQGRPARLPVDRVFSVEGFGTVVTGTLWQGTLRTGDRVTVE
                     PGNLSARLRSVQVHGHDVDAAYAGQRTAVALHGIDKSQVERGHWVVAPDSVVASFMID
                     ARLHVVPDAERPLANRQRIRFHLGASEILGRVVLLDKDELEPGASAMAQLRLEGPVAA
                     ERGDRFVLRSYSPQRAIGGGVVVLPNPEKHRRHDAGTVARLEVEEEGGPLERTAQALA
                     SCKGAIDVRSIAKAAALEADAAGDALDELVRDGRAHHLDDGRFLDAAAAGALESRLLE
                     AARAWQGVSVYRWGMPRGDLKSQLPREVDPALFDHLVNRLAAAGRLFVRDDRFRADTP
                     EPHLSERDAGRMALVRSRLGGGGFAPPTLKEMEADPALAPGLGEVLASLVAGGEVVKL
                     AADFYYPRATLESMASGLKAFFSGRTDMRVADLKDLFGISRKHAVPVLEYFDRLGVTR
                     RMGDVRVAGRLLAGDGGAA"
     gene            3546..4124
                     /locus_tag="VF720_07575"
     CDS             3546..4124
                     /locus_tag="VF720_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7879255.1"
                     /translation="MTERRPTVPVHDGPSDSALAVRAREGDGHAFKELVRRHQERVFR
                     IVHRMLRDRDQAEDVTQVTFVRAFRSLLKYKEEFPFHPWLYRIAVNASLTQIEKRKRE
                     KLVDIDLVPERFVPRPAGEESPLEVAGRRELVDRIRKALPELPEEQRVVFLLRVDEGF
                     SYDEIAQTLDIPRGTVMSRLSRAREALRKRLV"
     gene            4173..>5282
                     /locus_tag="VF720_07580"
     CDS             4173..>5282
                     /locus_tag="VF720_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX7879256.1"
                     /translation="MTEANFEGGHPGTEWLSAWHDGELQDEAAAAVRQHLDGCAACRH
                     LLDEFRQLDQATRLADDATPDAATWEGIEGRLRQRLEDELGLGIPVGAAARYVETDAE
                     LVMRPGLSEVRGGKGARPFRLRWAVATAAGLGLGLFTLRTLSDNGWTVSGVMDPAARH
                     RSEARVPARSAEATTASPQEPMTDNQADDKVQDGEVPPVPAAGTALPTGKIREEAGDA
                     GGLADAAPPPVVAAPMTDSGDRYLADVEQVLRAKESAASAGGAANESSPLEKDQAAPA
                     AARSAEGSSASRADADEAKKAKGEMSVEPEYAAVFAAASRARESGEYALARRGFALVA
                     GAVPDQPLARDAEFEARLAGAHADIVEGRSPDAVRS"
BASE COUNT          840 a         1777 c         1866 g          799 t
ORIGIN      
        1 ggtggtggtg aggggctggc ccggatcccc gccaacctgg aaatcgacag tctgcacggt
       61 gcccgtggcc ggggaggcgg tggcgctcgg gccgggcgtt acctttccgc cgaactcgcc
      121 gaacaggaag agaccgacaa aggcgcccag aaatgccgac agggcggcca cgcccaggat
      181 ttccctgcgt ttgatcatga atgaactccg ttgatgcggc cggccggggt ggccggcggg
      241 tccgaggatg atggccgggc aaatccccgc ccgtatggcg gttggctgag tcgaggcccg
      301 gcaacaagca tgttacactg cgcaacgttg gaaggtttca caccatctcc agggagctat
      361 ggacaagatt gtcgtccgcg gcggccgttc ccttagagga acgatcgagg ccgccgggtc
      421 caagaatgcc gcgctgccga tcctcacggc ggccatcctc gccccgggcg aataccgttt
      481 tacccatgtc cccctcctcc gcgatgtcac gaccatggcc cggctcctcg agacgctcgg
      541 cgccaccttc caccaggacg gccacaccct ccacctggac accagccggg tggagccgcg
      601 cgaggccccc tacgacctgg tcaagacgat gcgggcctct gtttatgttt taggtccttt
      661 gctggcccgt ttcggtgagg cccgggtgtc gctacccggg gggtgcgcct ggggcccccg
      721 ccccatcgac ctccatatca aggggatgac ggccctcggg gccgaaattt ccctcgagga
      781 ggggtatgtc gtggcccgtg cggaaaagct gacgggggcc gacatcgacc tggaaatcgc
      841 ctcggtggga gccaccggca acatcctgat ggccgcggtg gccgcctcgg ggatcacccg
      901 catccggaat gccgcgcggg agccggaaat tccggccctg gccgaattca tgaatgccat
      961 gggtgcccgg atcgaagggg ccggcacgag cgaaatcacc atcgagggtg gcgcgcccct
     1021 caagcccgcc ccctaccgga tcatcccgga ccggatcgag gtgggaacct acctgatcgc
     1081 cggggccatg accgccggag acgtgacgct gacggggtgc cgcccggacc atgtcggcgc
     1141 gctcctcatg cgcctgaaac agatcggcgc acgagtcgag gcggacgacg acaccatccg
     1201 cgtgatcggt cccgagcggc tcgattcgtt cgagttgcgc accgacttct atccgggctt
     1261 ccccaccgac ctccaggcgc agatgatggc gcttggcgcg gtggccaatg gccggagtgt
     1321 cctcaccgat accgtctaca ccgatcgatt cacccacgtc gcggaactgc agcgactcgg
     1381 agccgacatc cagatggaac ggaacgtcgc cgtcatcaac ggcctgaagc gtctctccgg
     1441 cgcccgggtg atggccaccg acctccgcgc ctcggcggct ctgatcctgg ccggattggt
     1501 ggccgagggt gagacccacg tctcgcgggt gtaccacatc gaccgcggct acgaacggat
     1561 cgaggagcgc ctcaccgcgc tcggcgccga tgtccggcgc gaaaccgaac aggggccgtg
     1621 acggggcgcc gcttcgccgt tctcggcacc gcgggacata tcgatcacgg caagaccgct
     1681 ctggtccgtc tcctcaccgg catcgacacc gaccggctga aggaggagaa ggaacgcggc
     1741 atctcgatcg accttggctt cgcccacctc tcccttcccg acggcaccga gtgcggcctg
     1801 gtcgacgtgc cgggacacga acgcttcgtg aaaaacatgc tggccggcgt cggcgggatc
     1861 gatgccgtcc tcttcgtcat cgccgccgac gagggggtca tgccgcagac gcgcgagcac
     1921 atggacattt tacgcctgct cgatgtcagg cgcggcgtgg tggcccttac caagagcgac
     1981 atggtcgatg ccgactggct cgacttcgtc acggaggagg tgcgcgactg gctggcgaag
     2041 ggccccctgg ccggggcgcc cattgtcccc gtgtcatcga agacgggcgc cggccgcgag
     2101 gcggtcctcg aggcgctggt ggcggcgctc gccgagctgc ccgaacggtc ccagggccgg
     2161 ccggcccgcc tgccggtcga tcgagtcttc agcgtggagg ggttcggcac ggtcgtcacg
     2221 ggcaccctct ggcagggaac cctgcgaacc ggtgaccggg tgacggtgga gcccggcaac
     2281 ctttcggccc gcctccgctc cgtccaggtg cacggccacg atgtggacgc ggcctacgcg
     2341 gggcaacgaa ccgcggtggc gctgcatggc atcgacaaat cacaggtgga acgcgggcat
     2401 tgggtggtgg cgcccgactc ggtcgtggct tcgttcatga tcgacgcgcg cctccatgtc
     2461 gttcccgacg ccgagcgccc cctggccaac cgccagcgaa tccgcttcca tctgggggcc
     2521 agcgagatcc tgggcagggt ggtcctgctc gacaaggacg agctcgagcc cggcgcctcc
     2581 gccatggcgc agctgcggct cgagggacca gtggccgccg aacggggcga tcgcttcgtg
     2641 ctccggagtt attcgccgca gcgcgccatc ggcggcgggg tggttgttct gccgaacccg
     2701 gagaagcatc gccggcacga cgcgggaacg gtggcccgcc tcgaggtcga ggaagaggga
     2761 gggcctctcg agcggaccgc gcaggcgctg gcatcctgca agggcgccat cgatgtccgg
     2821 tcgatcgcga aggcggcggc gctcgaggca gacgccgcgg gcgatgcgct cgatgaactc
     2881 gtacgggatg gtcgtgccca tcacctcgat gacggacgct tcctcgacgc agcggccgcc
     2941 ggcgcgctcg aatcccgcct gctcgaagcg gcgcgggcat ggcagggggt atccgtctat
     3001 cgctggggaa tgccgcgggg tgatctcaag agccagctcc cgcgcgaggt cgacccggcg
     3061 ttgttcgatc atctcgtcaa ccggctggcc gccgcggggc ggctcttcgt tcgggacgat
     3121 cgtttccggg ccgacacgcc ggaaccgcac ctttccgagc gggatgccgg gcgcatggcc
     3181 ctcgtccgga gccgcctcgg cggagggggc ttcgcgcctc ccacgctgaa ggaaatggaa
     3241 gccgatccgg ccctcgcccc ggggctcggc gaggtgctcg cctccctcgt ggcaggtggg
     3301 gaggtggtca agctggcggc agacttctac tatccccgcg cgacgctcga gtcgatggcg
     3361 tcgggactga aggctttctt ctccggacga accgacatgc gcgtggccga cctcaaggat
     3421 ctcttcggaa tttccagaaa gcacgcggtg ccggtgctcg agtatttcga ccggctcggc
     3481 gtgacccggc gcatgggtga cgtgcgcgtg gccgggcgcc tgctggccgg cgacggcgga
     3541 gcggcgtgac cgagcgtcgt cccacggtgc ccgttcacga cggtccgtcc gattcggccc
     3601 tggcggtccg cgcccgcgag ggtgacggcc atgccttcaa ggaactggtg cgccgccacc
     3661 aggagcgcgt cttccgcatc gtccaccgga tgctgcgcga tcgcgaccag gcggaggatg
     3721 tcacgcaggt gaccttcgtg cgggccttcc gcagcctcct caagtacaag gaggaatttc
     3781 cgtttcaccc gtggctctac cggatcgccg tcaacgcgtc gctcacccag atcgagaagc
     3841 ggaaacggga aaaactcgtc gacatcgacc tggtgcccga gcgtttcgtt ccgcggccgg
     3901 caggcgagga atcgcccctc gaggtggccg gaagaaggga actggtggac cggatccgga
     3961 aggcgcttcc ggagctaccc gaggagcaga gggtggtctt cctcctcagg gtcgatgaag
     4021 gcttcagcta tgacgaaatt gcccagacgc tggatatccc ccgggggact gtcatgtcgc
     4081 gcctgtcacg tgcccgggaa gccctgcgca aaagactggt ataggctctt ccggccgggc
     4141 gcgccgcccg ccggcaatcg aaaggaagca ccttgaccga ggcgaatttc gaggggggtc
     4201 acccgggcac ggaatggttg tcggcctggc acgacggcga gctgcaggac gaggcggccg
     4261 cggccgtccg ccagcatctc gacggttgcg ccgcctgccg gcacctcctc gacgaattcc
     4321 ggcaactcga ccaggccacc cggctggccg acgatgccac ccccgatgcc gcgacgtggg
     4381 aggggattga aggtcggctc cgccagcgcc tggaagacga gctcggtctc ggcatcccgg
     4441 tgggtgcggc ggcccgctac gtggaaaccg acgccgagct cgtcatgcgt cccggcctgt
     4501 ccgaagtgcg cggcggcaag ggagcgcgcc ccttccggct tcggtgggcg gtggccacgg
     4561 cagccggcct cggcctcggg ctgttcaccc tgcgcacatt gagcgacaac gggtggacag
     4621 tttcgggcgt gatggatccg gcggcccgac accgctcgga ggcgagggtg cccgcccgat
     4681 cggcggaggc gacgacggcc tcgccgcagg agccgatgac cgacaaccag gcggacgaca
     4741 aggtgcagga cggggaagtc ccgccggttc cggcggcggg caccgcgttg cccaccggca
     4801 agattcgtga agaagcgggc gatgcgggcg ggctcgccga tgctgcgccg ccgccggtgg
     4861 tcgccgcccc gatgacggac tccggggatc gatatctcgc cgatgtcgaa caggtgctgc
     4921 gtgcgaaaga atcggcggcg tccgcgggag gcgccgcgaa tgagagttcc cccctcgaga
     4981 aggatcaggc ggcgccggct gcagcccggt cggcggaggg aagtagcgcg tcccgcgccg
     5041 atgccgacga agcgaagaag gccaaggggg agatgagcgt cgagccggag tacgccgccg
     5101 ttttcgcggc ggccagtcgt gcccgggaat cgggcgaata tgccctggcc cgccgcggtt
     5161 tcgccctggt ggccggggcc gtcccggatc aaccgctcgc gcgcgatgcc gagttcgaag
     5221 cccgcctcgc cggcgcgcac gccgatatcg tcgagggacg gagtccggac gcggtgcgtt
     5281 cg
//
LOCUS       DASVOD010000336         2747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_700636, whole genome shotgun sequence.
ACCESSION   DASVOD010000336 DASVOD010000000
VERSION     DASVOD010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700636"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VF720_07585"
     CDS             complement(<1..438)
                     /locus_tag="VF720_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879257.1"
                     /translation="MSRMSLRRAPDCISSAVNSSETGTLIRFSIGADDAEVRLEVGAA
                     AAAEAVAAAGADEFLAGAGSTREIRVEEPDVTTAALGGVAAAVGGSAGDGIDMSNGAL
                     SFFLKPNIRASFWPKPGRSAGDSWTGTPPLPPKEPDDIALLADA"
     gene            593..1414
                     /locus_tag="VF720_07590"
     CDS             593..1414
                     /locus_tag="VF720_07590"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7879258.1"
                     /translation="MSPTTGFLLGLVQGLTEFLPMSSSGHLVLAEHFLGVTGGTAFFD
                     VFLHLATFLAAVVALRTELVRIMAFLASTMGIRWVSLRSDELREGRRLFLALVIGTIP
                     AAVAGLLFRDALSVLADSPRFVGFALLVTGLVLVATRLAPNGTAQLDATSALAIGFAQ
                     ALALLPGVSRSGMTVAAGLFAGVRRERVVRFSFLLSLPAVLATAVLKLRQEDLVMGQL
                     TPLTLAVAFVTALVSGFLALQLMVRVVVSGKLTFFGIYCIAVGVAALVFLPAARP"
     gene            complement(1797..>2747)
                     /locus_tag="VF720_07595"
     CDS             complement(1797..>2747)
                     /locus_tag="VF720_07595"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX7879259.1"
                     /translation="PHDLRAQVAERLAECSWELFVPIRDGQSLHAVAVFELARRAGGL
                     DSRELAVLRRISRSASDALAAARRHEDERERERLQGELDVARSIQRHLLPDEPPLAPL
                     VELAAITLPGEAVGGDAHDFSRLPDGRIGVAVSDVAGKGIPAAILMASVQASFRALSE
                     SGLDPGPLMAALNRRVLEINEPDRFVCFFFATIDPNGSELSFVNAGIEPPILIRDDGS
                     AFDLDGGGLVLGVLPGAAYEARGVNVQAGDVLLVFSDGLVDAKDPDGAPSNRTRLADV
                     VRRSPGLTAERYRELILKEARLNGDAATEDDVTILVARFY"
BASE COUNT          384 a          961 c          926 g          476 t
ORIGIN      
        1 ggcgtcggcc aacagcgcga tgtcgtccgg ttccttcggt gggagcggcg gcgtcccggt
       61 ccatgaatcg ccggcggatc gtcccggctt cggccagaag ctggcccgga tgttcggttt
      121 caagaagaag gaaagtgcac cgttcgacat gtcgatgccg tccccggccg agccgccgac
      181 cgcggcggcg acgcctccca gggcggctgt cgtcacgtcc ggttcctcga cccggatttc
      241 gcgcgtcgac ccggcgccgg ccaggaactc gtccgccccg gccgcggcta ccgcttcggc
      301 cgcagctgcc gctccgacct cgagccgcac ctcggcatcg tccgccccga tcgagaagcg
      361 gatcagcgtc ccggtctcgc tcgagttgac cgccgaggag atgcagtccg gcgcgcgcct
      421 caagctcatc ctggacatcg acctctccac gatgcaggcg acggtggagc gcgacacccg
      481 taccggtacg cgctaggcac ggggggcagt ccgcgccgcg ccgccggcca gtccgggact
      541 gtttaccact cagcttgacg gtccaacggg gcttgtctaa cgtgccccgc ctatgtctcc
      601 gaccaccgga ttcctgctcg gcctcgtcca ggggctgacc gagttcctcc cgatgtcgtc
      661 gtcgggacac ctcgtcctcg cggagcattt cctcggtgtg acgggcggca ccgccttctt
      721 tgatgtcttc ctccacctcg ccacgttcct ggccgcggtg gtggcgctcc gcaccgagct
      781 ggtccggatc atggccttcc tggcatccac catgggcatt cgatgggtgt cgcttcgatc
      841 ggacgagctg cgcgaagggc gcaggctctt cctcgccctc gtgatcggca cgatcccggc
      901 ggccgtggcc ggtcttctgt tccgcgatgc cctgtccgtt ctcgccgaca gcccgcgctt
      961 cgtcggattc gcgctgcttg tcaccgggct ggtcctggta gccacgcggc tcgcgcccaa
     1021 cggcacggcg cagctcgacg ccacctcggc cctggccatc ggtttcgcgc aggcgctggc
     1081 cctcctgccg ggcgtgtcgc ggtcggggat gacggtggcg gcggggctct ttgccggcgt
     1141 gcggagagag agggtggttc gtttctcatt cctcctgtcg ttgccggcgg tcctggcgac
     1201 cgccgtgctc aagctccgac aggaagatct cgtgatgggg cagctcacgc cgctcacgct
     1261 ggcagtggcc ttcgttacgg ccctggtgag cggatttttg gcgctccagc tgatggtgcg
     1321 cgtggtggtc tccgggaaac tgaccttctt cgggatctac tgcatcgccg tcggcgttgc
     1381 ggcgctggtg ttcctccccg ccgcacgtcc ctgacgccgg caccgcggct gcggcgccga
     1441 catcccgtgg acggtcccgc ctagcgcttc aaccaccaga agaagtcgaa gcacagcacg
     1501 atgaatggct ccatcgttcc ctccccgttt gttgcaccgg gggcgggatt gccggcggtt
     1561 gccggcaccc atacccgacc gggtgaattt gcataagccg ttccatttgc gatctgtctg
     1621 ataaataggc tgtaagctac tgtaattgaa agagttggtt cccgagtcgg cgtggcccgg
     1681 cacccttcgc cggccccagg ggacccggca tgccggtcgt cgaccggata ttgatcggtt
     1741 ggaaatcgac cgcttccggg atccaccgcc gcggaatcag cccgcagcca accgcctcaa
     1801 tagaagcggg ccacgaggat cgtcacgtcg tcctcggtgg ccgcatcgcc attgagccgt
     1861 gcctccttga ggatcagctc gcgataccgc tcggcggtga ggccagggga gcgtcggacc
     1921 acgtcggcca ggcgggtgcg gttgctcggc gcgccatcgg ggtctttcgc atcgaccagg
     1981 ccgtcggaga agaccagcag cacgtcgccg gcctggacgt tcacgccgcg cgcttcatag
     2041 gccgcgcccg ggagcacgcc gagcacgagg cccccgccgt cgagatcgaa agccgatccg
     2101 tcgtcacgga tcaggatcgg aggctcgatc ccggcgttca cgaacgagag ctcgctgccg
     2161 ttcggatcga tcgtggcgaa gaagaagcag acgaaccgat cgggttcgtt gatctcgagg
     2221 acgcgccggt tgagggccgc catgagcggc cccggatcga ggcccgattc gctcagcgcc
     2281 cggaagctgg cctgcacgct ggccatcagg atggccgccg ggattccctt gccggccacg
     2341 tcggagacgg ccacgccgat gcggccgtcg gggagccgcg agaagtcgtg ggcgtcgccg
     2401 ccgaccgcct cgccgggcag cgtgatggcg gccagctcga ccagcggtgc cagcggcggc
     2461 tcgtccggaa ggagatgtcg ttggatgctg cgggccacgt cgagttcgcc ctgcaggcgt
     2521 tcgcgctccc gctcgtcctc gtgtcgtcgg gcggccgcca gcgcatccga tgccgagcgc
     2581 gagatgcgac gcagcaccgc cagctcgcgg ctgtcgagtc caccggcccg gcgagccagc
     2641 tcgaagacgg ccacggcgtg gagcgactgg ccgtcacgaa tcggcacgaa cagttcccag
     2701 ctgcactcgg caagccgctc ggccacctgg gcgcgcaggt cgtgcgg
//
LOCUS       DASVOD010000337         3035 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_335666, whole genome shotgun sequence.
ACCESSION   DASVOD010000337 DASVOD010000000
VERSION     DASVOD010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335666"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2732
                     /locus_tag="VF720_07600"
     CDS             <1..2732
                     /locus_tag="VF720_07600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879260.1"
                     /translation="FEAERQALALMDHPNIARVFDGGMTQSGRPYFVMELVRGTPITE
                     YCDREQLSIPERLDLFVLVCRAVQHAHQKGIIHRDLKPGNILVDEAGQPRILDFGVAR
                     STAADLTSGGATSAGDILGTLPYMSPEQVTLDPAAIDVRSDIYALGVVLYELLAGRLP
                     YDVRGKVMLEAMRVITEDDPAPLSTVDKLYRGDIETIVAKALEKERERRYDSAAELGA
                     DIRRHLDEEPITARPPSTVYQLRKFARRNRALVGGVAAVFLVLLAGVGISTWQAVRAR
                     RAERLASREAAKAAAVNEFLQSMLRAASPDQSPGRDMTVREMIDVAARRAGEDGPGAT
                     PEVAATVRSTLGDVYITLDRLDSAAVQLAAAMAIYRDAGLDRTADGNLARLDLANVYS
                     ERSRRDEAARLVEEARPIAEARRPPDFRELASLRGQQCRVRYYAGNFVDALALQREAL
                     EFRRRVPGVKPEQIAEELDALGFYHSELSEYDAADSLARDALRILTATYGTDHSKTLS
                     GYVKLADVQLLKLDLAGVDSTLQIGKAAARRLYGDKDSRIMADFLWREGRLLNARHEY
                     ARADSVHRICLAMREHVLGPNHNDVATSMSSVAGQTARFGNLAQADSLYRAAMDIRVR
                     EFGEDSPAVLASWNDVASLYEMYGQRERADSVYVAVVPRWRNHPGNEGNGEFMAITGA
                     ALNRQAMGDLPTSEMWFRRALAICREALGEDNLMVAKCLDNLGPSLFFQGRKDSAEIM
                     IRQALEIRRRKAPNTRIHALSLSNLGVILDDRKDYAGAESLYVEQLDIARNHEPSKLV
                     DAMSQLGRNYLFQEKWEPAEALLREAVELQAKRGETDRVSILMNQARLGRALVGLKRY
                     AEAEPLLVPVVPELAGKVPMRRAKLALVWLADYYDATNQPALAKEWRDKAAAG"
     gene            complement(2729..>3035)
                     /locus_tag="VF720_07605"
     CDS             complement(2729..>3035)
                     /locus_tag="VF720_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7879261.1"
                     /translation="AAASDPWTEVILARINHKGGPDWSMDASSNVVADVLRRARGNGK
                     GVVGMKLFAAGKMTSAADREASLRWTFERNLVDAVTIGTVSTAQIDENLAMVARARG"
BASE COUNT          490 a         1091 c         1005 g          449 t
ORIGIN      
        1 gcttcgaggc cgagcgccag gcgctggccc tgatggacca ccccaacatc gccagggtct
       61 tcgacggcgg gatgacccag tccggtcgcc cctatttcgt gatggagctg gtgcgcggca
      121 cccccatcac cgaatactgc gaccgcgagc aactttcgat cccggagcgg ctcgacctgt
      181 tcgtgctggt ctgccgggcc gtccagcacg cccaccagaa ggggatcatc caccgcgacc
      241 tgaagccggg gaacatcctg gtggatgaag ccggacagcc acggatcctc gacttcggcg
      301 tggctcgctc cacggccgcc gatctgacga gcgggggcgc aaccagcgcc ggcgacatcc
      361 tcggcaccct cccatacatg agtcccgagc aggtcacgct cgacccggcc gccatcgacg
      421 tacgcagcga catctatgcc ctcggcgtgg tgctctacga gctcctggcg ggccgcctgc
      481 cgtacgacgt gcgcggcaag gtgatgctcg aggcgatgcg cgtcatcacc gaggacgacc
      541 cggcgccact ctcgacggtc gacaagctct atcgcggcga catcgagacg atcgtggcca
      601 aggctctgga aaaggaacgc gagcgccgct acgactcggc ggccgagctc ggtgccgaca
      661 tccgccgcca cctcgacgag gagccgatca cggcgcgccc ccccagcacc gtttaccagc
      721 ttcgaaagtt cgccaggcgg aaccgggcgc tcgtgggtgg cgtggcggct gtctttctcg
      781 tgctgctggc cggtgtcggc atctccacct ggcaggccgt gcgggcgcgc cgggccgagc
      841 ggctggcctc gcgcgaggcg gccaaggcgg ccgccgtcaa cgaattcctc cagtcgatgc
      901 tgcgggccgc ctcgccggac caatcgccgg ggcgcgacat gaccgtgcgg gagatgatcg
      961 acgtggcggc ccgccgggcc ggcgaggacg gccccggcgc cacgcccgaa gtagccgcca
     1021 ctgtccggtc cacgctcggc gatgtctaca tcacgctgga ccggctcgac tcggccgccg
     1081 tgcagctcgc cgccgccatg gccatctacc gcgatgccgg actcgaccgc accgcggatg
     1141 gaaacctggc ccgcctcgac ctggccaacg tgtactccga acgcagccga cgggacgagg
     1201 ccgcaaggct ggtggaggag gcccggccca tcgccgaggc gcgccgcccc cccgacttcc
     1261 gggagctggc gtcgctgcgc ggacagcagt gtcgcgtccg ctattacgcc ggcaacttcg
     1321 tcgacgcact ggcgctccag cgcgaggcgc tcgagttccg ccggcgcgtg ccgggggtga
     1381 aaccggagca gattgccgag gagctcgacg ccctggggtt ctaccactcc gagctgagcg
     1441 agtacgacgc cgccgactcg ctggcccgcg acgccctccg catccttacc gccacctatg
     1501 gaaccgacca ctcgaagacg ctgtcgggtt acgtcaagct ggccgatgtc cagctcctga
     1561 agctcgacct ggccggtgtc gactccaccc tccagatcgg caaggccgcc gcccggcgcc
     1621 tgtacggtga caaggactcg aggatcatgg ccgacttcct gtggcgcgag gggcgccttc
     1681 tcaacgcccg ccatgaatac gcccgggccg actcggtgca ccggatctgc ctggccatgc
     1741 gcgagcacgt gctcgggccg aaccacaacg acgtggccac ctccatgtcc tcggtggccg
     1801 gccagacggc caggttcggc aacctcgccc aggccgactc gctgtaccgt gcggccatgg
     1861 acatccgggt gcgtgaattc ggcgaggatt cgcctgccgt gctggcttcc tggaacgacg
     1921 tggcgtcgct gtacgagatg tacgggcagc gtgagagggc cgactcggtc tacgttgccg
     1981 tggtacccag gtggcgtaac catcccggca acgaggggaa cggcgagttc atggcgatca
     2041 ctggcgccgc cctgaaccgg caggccatgg gcgacctgcc cacttcggag atgtggttcc
     2101 ggcgcgcgct cgccatctgt cgggaggccc ttggcgagga caacctgatg gtggccaagt
     2161 gcctcgacaa ccttggcccc tcgctcttct tccaggggcg caaggactcg gctgagatca
     2221 tgatccggca ggcgctcgag atccgtcgcc gcaaggcgcc gaacactcgc atccacgcgc
     2281 tgagcctctc caacctgggc gtcatcctcg acgatcggaa ggactacgcg ggcgccgaat
     2341 cgctctacgt ggaacagctc gacatcgcca ggaaccacga gccatccaag ctggtcgacg
     2401 ccatgagcca gcttggtcgc aactacctct tccaggagaa gtgggaaccg gcggaggcgc
     2461 tccttcgcga ggccgtcgag ctccaggcga agcgcggcga aacggaccgc gtgtcgatcc
     2521 tgatgaacca ggcccgcctg ggacgggcac tcgtcgggct caagcgctac gcggaggccg
     2581 agcccctgct cgttcctgtc gtgcccgagc tcgccggaaa ggtgccgatg cgccgggcga
     2641 agctggccct cgtctggctg gccgactact acgacgccac aaaccagccg gcactcgcga
     2701 aggagtggcg ggacaaggcg gcggcgggct agccgcgcgc ccgcgccacc atcgccaggt
     2761 tttcgtcgat ctgcgcggtg ctgacggtgc cgatcgtcac ggcatcgacg agattccgtt
     2821 cgaacgtcca gcgcagcgag gcctcgcggt cggcggccga cgtcatcttc ccggcggcga
     2881 acagcttcat gccgacgaca cccttgccgt tgccgcgcgc ccgccgcaac acgtcggcca
     2941 ccacgttgct cgaggcgtcc atggaccagt cgggcccacc cttgtggttg atgcgcgcca
     3001 ggatcacctc cgtccacgga tcgctggcgg cggcc
//
LOCUS       DASVOD010000338         3304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_858128, whole genome shotgun sequence.
ACCESSION   DASVOD010000338 DASVOD010000000
VERSION     DASVOD010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858128"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF720_07610"
     CDS             <1..460
                     /locus_tag="VF720_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879262.1"
                     /translation="TASNKEYVPVVQQAIATRLDDMKVMTAHSGVTKVSSGSSCCAGK
                     ASTASMTTGASCATSASATKVSDGAGCATTASATKVSDGATCATKASATTSASASMES
                     CPEWMKVLCTANCTVENTATGVKVIWTTPEKNKVEQVQMAGEQLHADLAS"
     gene            694..769
                     /locus_tag="VF720_07615"
     tRNA            694..769
                     /locus_tag="VF720_07615"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:727..729,aa:Ala,seq:ggc)
     gene            complement(826..1575)
                     /locus_tag="VF720_07620"
     CDS             complement(826..1575)
                     /locus_tag="VF720_07620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879263.1"
                     /translation="MSTPTPAQAARATIERIDPHLIDTTKEGPRTTLDLHLARYEFAA
                     GHAAGRRVLDCACGVGYGSALLKEKGQASEVLGVDIDQGAIRRAGEAYARPGVTFTCK
                     SAYDLSTAEPFDMAVSLETIEHLPDPEGFLDILHRLTKPGAILVGSVPVTPSVDVNIH
                     HLHDFTEGSFRKLLRRHGFEPFEERLQRQPYSLFSLRKEIQSKAWGLDIRPNLPGYYL
                     RNPGKAWLRFCHMLKDGTVNKYLVVAARRVD"
     gene            1735..2361
                     /locus_tag="VF720_07625"
     CDS             1735..2361
                     /locus_tag="VF720_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX7879264.1"
                     /translation="MDFDLRSLLLNIPGILFGFTIHEFAHAWTAWKLGDDTAERQGRL
                     TLNPLAHLDPMGTLLILFAGFGWARPVPVNPARFQHPRRDDVLVTLAGPASNLVTAGL
                     MAVAYRLIPAGGAESLSGAFSELLYRGIYFNLLLCFFNLIPIFPLDGARILRGLLPLN
                     QAYAFSKLESVGPLILLAIIALGSYARIDILGRIIGPPIKLFMGVFTT"
     gene            2361..>3304
                     /gene="trpS"
                     /locus_tag="VF720_07630"
     CDS             2361..>3304
                     /gene="trpS"
                     /locus_tag="VF720_07630"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX7879265.1"
                     /translation="MAKGRILSGMRPTGRLHLGNFTGALDTWVKLQDEFECYYMVADW
                     HALTTDIEHTRQIRERTFEMVVDWLAAGIDPERSPVFVQSHVPEHAELHLIFSMLTTV
                     ARLERNPAVKEQARDLGLEANITYGHLGYPVLQAADILLYKGNVVPVGEDQVPHVEIT
                     RETARRFNAVFGQVFPEPEARLTPSARFPGLDGKRMSKSLGNVVMVSESPEEIQKKVR
                     TAYTDPGKVRRNDPGRPIQSGCVVYSWHGRFNAAEQPTILADCESGALGCVACKTRIS
                     EVLAAEFAPLREKREAFLADPDRVWGILEAGRDRARAVA"
BASE COUNT          544 a         1112 c         1077 g          571 t
ORIGIN      
        1 caccgcctcg aacaaggaat atgtgccggt cgtccagcag gccatcgcca cccgcctcga
       61 cgacatgaag gtcatgacgg cccattcggg cgtgaccaag gtttcttccg gcagctcctg
      121 ttgcgccggc aaggcttcga ccgcctcgat gacgacgggt gccagctgcg ccacgtcggc
      181 gtcggccacg aaggtctccg acggcgccgg ctgcgccacg accgcctcgg ccaccaaggt
      241 ttccgacggc gccacctgcg ccaccaaggc cagcgccacc acgtcggctt cggcctcgat
      301 ggaaagctgc cccgagtgga tgaaggtgct ctgcacggcg aactgcacgg tggaaaacac
      361 cgccaccggc gtcaaggtga tctggacgac tcccgagaag aacaaggtgg agcaggtcca
      421 gatggccggc gagcagctcc acgccgacct ggcgagctga tcacgtcggg ccggcggtga
      481 cgccgggctg atgacgtcga agaacctgat tggaatacag gacttgaatg gcaattctcg
      541 cggggcctcg gaaggggtcc cgcgattcat ttgaagcggg cgccgtagga ggcgccgttt
      601 acgacgcctg acgcgcctga tgtgcttgat gtgcttgacg cccgccgtcg acccgcctag
      661 aatccccggc tcgcaatatc acccaaccat ttcggggctg tagctcagtt gggagagcgc
      721 ctgactggca gtcaggaggt agtgggttcg aaccccatca gctccaccat tacatggcaa
      781 agggggtccg gttgtccgga ccccctttgt cgttgctgac ccgccttagt ccacccgtcg
      841 cgccgccacc accaggtact tgttcaccgt gccgtccttc agcatgtggc agaagcggag
      901 ccaggccttg ccggggttcc ggaggtagta gccggggagg ttggggcgta tgtcgaggcc
      961 ccaggccttc gactggattt ccttgcgcag cgagaagagg gaatagggct ggcgctggag
     1021 gcgttcctcg aacggctcga agccgtggcg gcggaggagc tttcggaacg acccctcggt
     1081 gaagtcatgg agatggtgga tgttcacgtc cacgctcggc gtgaccggca cggagccgac
     1141 caggatggcc cccggcttcg tcagccggtg caggatgtcg aggaaccctt cgggatcggg
     1201 gagatgctcg atcgtctcga ggcttacggc catgtcgaac ggctcggccg tcgagagatc
     1261 gtaggccgac ttgcaggtga aggtgacgcc cgggcgcgca taggcctcac cggcccggcg
     1321 gatcgccccc tggtcgatgt ccacgcccaa cacttcgctg gcctggccct tctccttgag
     1381 gagggcgctt ccgtacccca cgccgcaggc gcaatcgagc acgcggcgtc cggccgcgtg
     1441 gccggccgcg aactcgtagc gcgccaggtg gaggtcgagc gtggtgcgcg gaccttcctt
     1501 tgtcgtgtcg atgagatggg gatcgatgcg ttcgatggtg gcgcgcgcgg cctgggcggg
     1561 ggtcggggtg ctcacgcggg gggcctcatg gagcgtcgat tctcccatcc gcccgccgat
     1621 cgggtcaacg aaaacgtgac aggatcaccc cttatgccgt tctcacgcgg ctgttagaat
     1681 cgcccgccaa tcaagctccg gctcgttgtt tcggtcagga aaggcacgcg tcacgtggat
     1741 ttcgaccttc gatccctcct tctcaacatc ccgggaatcc tcttcgggtt cacgatccat
     1801 gaattcgcgc atgcctggac ggcctggaaa ctgggggacg acaccgcgga gaggcagggg
     1861 cgcctcaccc tgaacccgct ggcccacctc gatccgatgg ggacgctgct catcctcttc
     1921 gccggcttcg ggtgggcccg gccggtcccg gtcaatccgg cccggttcca gcatccgcgc
     1981 cgcgatgacg tcctcgtcac gctggctggc ccggcctcga acctcgtgac cgcggggctc
     2041 atggcggtgg cctaccggtt gatcccggcc ggcggcgccg agtcgctctc cggcgcgttc
     2101 agcgaactgc tctatcgcgg gatctatttc aatctgctcc tctgcttttt caacctgatc
     2161 ccgatcttcc cgctcgacgg ggcgcgcatc ctccggggac tgttgcccct caaccaggcc
     2221 tacgcgttca gcaagctcga gtcggtcggg ccgctcatcc tgctggcgat catcgcgctc
     2281 ggctcgtacg ccaggatcga catcctcggc cggatcatcg gcccgccgat caaactcttc
     2341 atgggggtct tcaccacctg atggccaagg gacgcatcct ctccggcatg cggccgaccg
     2401 gccgcctcca cctcggcaac ttcaccgggg cgctcgacac ctgggtcaag ctccaggacg
     2461 agttcgagtg ctactacatg gtggccgact ggcacgccct gaccacggac atcgagcaca
     2521 cccgccagat ccgggagcgc accttcgaaa tggtggtcga ctggctggcg gccggcatcg
     2581 acccggagcg gagcccggtc ttcgtccaga gccacgtgcc ggagcatgcc gagctgcacc
     2641 tcatcttttc gatgctcacc acggtggccc gcctcgagcg gaaccccgcg gtcaaggagc
     2701 aggcgcgtga ccttgggctc gaggccaata tcacttacgg ccacctgggg tatcccgtcc
     2761 tccaggccgc cgacatcctc ctctacaagg gaaacgtcgt gccggtgggg gaggaccagg
     2821 tgccgcacgt cgagatcacg cgggaaaccg cccggcggtt caatgccgtc ttcggccagg
     2881 tctttcccga gcccgaggcg cgcctcaccc cctcggcccg cttccccggg ctggacggca
     2941 agcggatgtc gaaatcgctc ggaaacgtgg tgatggtgtc cgaatcgccc gaagagatcc
     3001 agaaaaaggt tcgcaccgcc tataccgatc cgggtaaagt gcggcggaac gatccggggc
     3061 gcccgatcca gtccggctgt gtcgtctatt cgtggcacgg acggttcaac gccgccgagc
     3121 agccgaccat cctggccgac tgcgagagcg gcgccctggg atgcgtggcc tgcaagaccc
     3181 ggatctccga ggtgctggcc gccgagttcg ctcccctgcg cgaaaaacgg gaagccttcc
     3241 tggccgatcc ggaccgcgtg tggggaatcc tcgaagccgg ccgcgaccgg gcgcgggccg
     3301 tggc
//
LOCUS       DASVOD010000339         7508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1016819, whole genome shotgun sequence.
ACCESSION   DASVOD010000339 DASVOD010000000
VERSION     DASVOD010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016819"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VF720_07635"
     CDS             complement(<1..577)
                     /locus_tag="VF720_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7879266.1"
                     /translation="MKGIRGAALVAVAALALAGCSDSSTDSKDVGPYPNSDLLVSGAS
                     LATNLAAPGQIIVDTRSATAYAAGHIPGAINIPITPGNGTFDKGGSGPDATDLKPPAE
                     LAAALGAAGIRESNHVVVCGTDIDWLAGRLFWMLEYLGAPKVSMLDGGFSKWAADGRT
                     TSTAATTLPPATFTPHVVATRLVDKTDVLAHY"
     gene            complement(612..1493)
                     /locus_tag="VF720_07640"
     CDS             complement(612..1493)
                     /locus_tag="VF720_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879267.1"
                     /translation="MTGAQGEGGDERPRIPWGVWIALLLGALLAAAISTRTARAGAWT
                     QEKGALYNRLAGSFYGSTEEYDTEGHRDFIPFHGDYAEVNLTDYVEYGLTDRLTVLGS
                     LVLKKLELENDIRITRSKGLGDIDAALRFKLAEGSAGVASVQALVKIPTGYEVDVPLP
                     IGNGEFEYDAHLLYGRSLWPLIPGYCGVEVGYRWRAGAPEDEIRYLAEVGTDLGKHLY
                     TRAKLDGVKGTKSGAAVDLNGNPTIRNAYDLGTLDLTFGGKLGSHWFLEGGYAPSLYG
                     RTTTAGSRWSLALACTL"
     gene            1629..2612
                     /locus_tag="VF720_07645"
     CDS             1629..2612
                     /locus_tag="VF720_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HEX7879268.1"
                     /translation="MNLRQLDVFLAVVEEGSFSGAARVAMLTQSTISQHMAALEEELG
                     VQLLERSRNGVVLTEAGRILRKHARSLVGELRATETAMRRHRGIEQTSLRLGVSTIPG
                     AYMVPTVLAKLGEKFPQLNVVVLQGDSRTTVEWLAAREIEAGVVGKRFEERGFTFAEV
                     GEDRISLVVGRQHPWARRGTLTARDLYDGVFVAREPGSGTGATVTEALRDAGIDTARL
                     RTRAEIGGMEAIKAAVLAGVGASFLSHVAVASDVERGDLVIVPVKGLTIARPFYLVRR
                     SGRDLAPAAEAFWKLMIEVYGRSGAPSTAKGSARKGAGRVAGRRTPRTTGR"
     gene            complement(2609..4669)
                     /locus_tag="VF720_07650"
     CDS             complement(2609..4669)
                     /locus_tag="VF720_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7879269.1"
                     /translation="MTGPAAGGRRNRLASATSPYLLQHAANPVDWYPWGEEALERARR
                     EDKPIFLSIGYAACHWCHVMEHESFEDAGIAALLNESFVCVKVDREERPDLDEIYMCA
                     VQLLSGSGGWPMTVFLTPELAPFYGGTYFPPDDKWGRLGMRRLIPEVARVYREQREQV
                     AEQAADLTQAVRTIVGAMNATDGDGAIVGELPGRAVLDAAVARLLAAHDDEWGGFGAA
                     PKFPPSMGLSVLLHHHHRTGDARTAAVVRTTLDRMARGGMYDHLGGGFARYSVDERWQ
                     VPHFEKMLYDNALLVSVYAEAFRVIGDTPDRQYRRTIDESLAFVQRELTDPAGGFHSS
                     LDADSDGEEGRFYVWTRPEIESVLGPADADVFCRAFDVTLEGNWEHGNSVLWQPLSVG
                     DEVRHAVAPMRERLLAARAARNRPGLDDKVLAAWNGLMISAFVDASLALDDARWLEPA
                     RKAARFVLDRMMINGRLLRSWRSGTAGLTAYVEDYAAMAGACVDLYEATFEVEWLSAA
                     EALVTTAMAHYADPVGGFFLTADDAEALLVRPKSAQDGATPSGNALMAVVLLRLAALL
                     DRPDWRAAAEATVKAHAASLATIPGAHHRMLLAVDWLTGDPVEVAIVGAPDDTGTKAL
                     LDAARSARPYHRVFASAAAASAALPLLRDRHPIDGRPTAWVCRQFTCRAPVRRPEELV
                     AELR"
     gene            complement(4686..5852)
                     /locus_tag="VF720_07655"
     CDS             complement(4686..5852)
                     /locus_tag="VF720_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7879270.1"
                     /translation="MAIPISYNLRNLFVRRLATSITVIGIALVVAVFIAVLALAQGFQ
                     RALAGNGLDNNAIVLRVPGDAELSSSVSREWVSIIETQPEVAVDSDGRPMVVPELVVV
                     VNLKKKSTGGIANILTRGTNPRALALRPRLKLMAGRLWESGKSEVVVGKLISERFQGC
                     ELGEQITFGGRAWTVVGILDADGTSFESEVWGDSEILGPAFDREGYQSASLRLKDAAQ
                     FEALEARVEGDRRMQAIVKRESQYYADQSMQVSGMIQGLGMFISIIMAFGAVFGALNT
                     MYAAVSSRRSEIGTMMALGFSRSSVLLSFVLEALLLSSIGGILGGFLALPINGISTGT
                     TNWATFSEVAFRFTVTPVMLVIGFLFALVMGLIGGFFPALNAARSNIAQTVRRA"
     gene            complement(5852..7006)
                     /locus_tag="VF720_07660"
     CDS             complement(5852..7006)
                     /locus_tag="VF720_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7879271.1"
                     /translation="MKFLGLIFRNMTHSRRRTILTILSIAVALFLFATLRTVLTAFNA
                     AIEVADDTRLIVRRSTSLVFPLPVAYRDRIAGVNGVTAVSWGNWFGGIYQDPQKFFAK
                     FGVDVDTYFDMYPELLLPPEQLAAFKADRTGCVVGSGLAKKYGFKVGDTLPIVGTIYP
                     YPDGNEWRFTVRGIYTPETPDVDDLSMYFNWDYLNQTMGDPGIVGFYIVKLADSGQTA
                     VVGDRIDGLFANSPYETKTETEAAFQAGFLSMIGNIGFLVSIIGAAIVFAIALVTFNT
                     MMMAARERTKEIAVMKTLGFSDGSILGLIVAEATFISLIGGALGIGLATFLYWATGFD
                     LMGFVPSFLVKPATIGYGLLLSLGMGVLSGLIPALQAARMPISRALREGV"
     gene            complement(7003..>7508)
                     /locus_tag="VF720_07665"
     CDS             complement(7003..>7508)
                     /locus_tag="VF720_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005984398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7879272.1"
                     /translation="IVVEGEEISAMSGSKLAAWRARHVGFIFQFYNLIPVLDAYHNVE
                     LPLLLTPLSRSQRHDHVMTALRLVDLEDRMKHYPKQLSGGQEQRVAIARAIVTDPTLI
                     VADEPTGDLDARSADEILTLLERLNKEFQKTIIMVTHDRTAAQRAHQVRTFEKGVMGV
                     MEATVRA"
BASE COUNT         1260 a         2536 c         2501 g         1211 t
ORIGIN      
        1 cgtagtgggc cagcacgtcg gtcttgtcca ccaggcgcgt tgccacgaca tggggcgtga
       61 acgtggcggg cggaagcgtg gtggctgccg tgctggtcgt ccggccgtcg gcggcccact
      121 tcgagaagcc gccgtccagc atcgacactt tcggcgcgcc gaggtactcg agcatccaga
      181 agaggcgccc ggccagccag tcgatgtcgg tgccgcagac gaccacgtga ttgctttcgc
      241 ggatcccggc cgcacccagc gccgccgcca gctcggccgg gggcttcagg tcggtggcgt
      301 ccggtcccga gccccccttg tcgaacgtgc cgttgccggg cgtgatcggg atattgatgg
      361 ctccgggaat atgcccggct gcgtaggcgg tggccgaccg cgtgtccacg atgatctggc
      421 cgggcgccgc caggttcgtg gccagcgagg cgccggacac gaggaggtcg gagttgggat
      481 acggcccgac atccttcgag tcggtggagg aatcgctgca gccggccagc gcgagcgcgg
      541 ccacggccac gagagccgcc ccgcggatac ctttcatgat cctcatcatc tctattgctc
      601 ctcttgaacg tttacaacgt acaggccaat gccagtgacc acctggaacc ggcggtcgtc
      661 gtccggccgt acagcgacgg tgcgtaaccg ccttcgagaa accagtgcga accgagcttg
      721 ccgccgaatg tcaggtccag cgtgcccaga tcgtatgcgt tgcgaatggt ggggttgccg
      781 ttcaggtcga ccgcggcgcc gctcttcgtc cccttcacgc cgtcgagctt cgcgcgggta
      841 tacaggtgct tcccgaggtc ggtcccgact tcggccaggt agcggatttc gtcctccggc
      901 gcgcccgcgc gccagcgata accgacttca accccgcagt atccggggat caggggccac
      961 aacgaccggc cgtacagcag gtgcgcgtcg tactcgaact cgccgttccc gatggggagt
     1021 ggaacatcca cctcgtaccc ggtggggatc ttgaccagtg cctgaacgga ggccacgccg
     1081 gccgatccct cggccagctt gaagcggagc gcggcgtcga tgtcgcccaa ccctttcgag
     1141 cgggtgatgc ggatgtcgtt ttccagttcg agcttcttca ggacgaggga accgaggacg
     1201 gtcaggcggt cggtcaggcc gtactccacg tagtcggtga ggttcacctc cgcgtaatcc
     1261 ccgtgaaacg ggatgaagtc gcggtgcccc tccgtgtcgt actcctccgt actgccgtag
     1321 aagctgccgg ccaggcggtt gtagagggct cccttctctt gcgtccaggc acccgcccgg
     1381 gccgtccggg tggagatggc cgcggccagc aacgccccga gaaggagcgc aatccacacc
     1441 ccccatggaa ttcgcggcct ctcgtcccca ccctctccct gggcgcctgt cacgggttct
     1501 cctcgccggt tcttccgcct gccatcttgt ttcgagaaag aattagaccc atgctctgcg
     1561 cgctcggtca aattgatctt ttcaattgat cgatgggatc catcggtttt actgataggg
     1621 aggtccgggt gaacctgcga cagctggatg tcttccttgc cgtggtggag gaggggagtt
     1681 tttcgggcgc ggcccgcgtg gccatgctca cgcaatccac catcagccag cacatggcgg
     1741 ccctggagga ggagctcgga gtgcagctcc tcgagcgatc acgcaacgga gtggtgctca
     1801 ccgaggccgg acgtatcctg cgcaagcatg cccgaagcct ggtcggggag ctgcgcgcca
     1861 ccgaaacggc catgcgccgg caccggggga ttgaacagac atcgctgcgc ctcggcgtca
     1921 gcacgattcc cggggcctac atggttccga ccgtgctggc caagctcggc gagaagtttc
     1981 cccagctgaa cgtggtggtg ctccagggag attcccgcac caccgtggag tggctggcgg
     2041 cccgcgagat cgaggccggc gtggtcggca agaggttcga ggaacgcggc ttcacgttcg
     2101 cggaagtggg cgaggaccgc atcagcctgg tcgtcggacg acagcacccg tgggccaggc
     2161 gcggcacgct gaccgccagg gacctgtacg acggcgtctt cgtggcgcgc gaacccggct
     2221 ccggcacggg ggccacggtc accgaggcgc tgcgtgacgc cgggatcgac accgcccgcc
     2281 tgcggacgcg cgccgagatc ggcggcatgg aggccatcaa ggcggccgtg ctggccggcg
     2341 ttggcgcttc gttcctctcg cacgtggccg tagccagcga tgtcgaacgg ggcgacctcg
     2401 tgatcgtgcc cgtgaagggg ctcacgatcg cccgaccgtt ctacctggtg cgccgctccg
     2461 ggcgcgacct ggccccggcc gcggaggcgt tctggaagct gatgatcgag gtttacggcc
     2521 gcagcggcgc cccatcgacc gcgaaaggaa gcgcgcggaa aggggcgggc agggttgccg
     2581 ggcgccgcac gccgcgcacc accggccgtt agcgcagttc cgccaccagt tcctcgggtc
     2641 gccggacggg cgcccgacag gtgaattgcc ggcagaccca cgcggtgggg cgcccgtcga
     2701 tcggatgacg gtcgcgcagg agcggaagcg ccgcactcgc ggccgccgcg gaggcaaaca
     2761 cccggtgata ggggcgcgcg gaccgcgcgg catcgagaag cgctttcgtc cccgtgtcgt
     2821 cgggtgcacc cacgatcgcc acctccacgg gatctccggt gagccaatcc accgccagga
     2881 gcatgcggtg atgcgcgccg ggaatcgtgg ccaggctggc cgcgtgcgcc ttgacggtgg
     2941 cctcggcggc cgcccgccag tcgggccggt cgaggagcgc cgccaggcgc agcagcacga
     3001 cggccatcag ggcattcccc gacggcgtag cgccatcctg ggcgctcttc ggacggacaa
     3061 gaagcgcttc ggcatcgtcg gccgtgagga agaatccgcc gaccggatcg gcatagtggg
     3121 ccatcgccgt ggtcacgagc gcctccgcgg ccgacagcca ctcgacctcg aacgtggctt
     3181 cgtacaggtc gacgcacgcc ccggccatcg cggcgtagtc ctccacgtag gcggtcagcc
     3241 cggccgtgcc ggaccgccat gagcgcagga ggcggccgtt gatcatcatc cgatcgagga
     3301 cgaagcgtgc cgccttccgg gcgggctcca gccaccgcgc gtcatccagc gccagggagg
     3361 catcgacgaa cgccgagatc atgaggccat tccacgccgc cagcaccttg tcgtccaggc
     3421 cgggacggtt gcgggccgcg cgtgcggcca ggagccgctc ccgcatcggc gccacggcgt
     3481 ggcgcacctc gtcgcccacc gaaagtggtt gccagagtac ggaattgccg tgctcccagt
     3541 ttccctcgag cgtcacgtcg aaggcccgac agaagacatc ggcgtccgcc gggccgagca
     3601 cggattcgat ctccggccgg gtccacacgt agaagcgccc ttcctcgcca tccgaatcgg
     3661 cgtcgagcga agaatggaac ccgccggccg ggtccgtcag ctcacgttgc acgaacgcca
     3721 gcgattcgtc gatggtgcgg cgatactgcc tgtccggcgt gtcgccgatc acccggaacg
     3781 cctcggcgta cacgctcacc aggagcgcgt tgtcgtacag catcttctcg aagtgcggca
     3841 cctgccaacg ctcgtcgacc gagtagcggg cgaacccgcc ccccaggtga tcgtacattc
     3901 caccgcgcgc catccggtcg agcgtggtgc gcaccaccgc cgcggtgcgc gcatccccgg
     3961 tccggtgatg atgatgcagg agcaccgaca gccccatgct cggcggaaac ttgggcgcgg
     4021 caccgaaccc gccccactcg tcgtcatggg ccgccagcaa tcgggccacc gccgcatcga
     4081 gcaccgcgcg cccgggaagt tcgccgacga tcgcgccgtc gccatccgtt gcgttcatcg
     4141 cgcccacgat cgtgcgaacg gcctgcgtca ggtcggcggc ctgttccgcc acctgctcgc
     4201 gctgctcccg gtagacacgc gccacttcgg ggatcagccg gcgcatcccg aggcgccccc
     4261 acttgtcgtc cggcggaaag taggtgccgc cgtagaacgg tgcgagctcc ggcgtgagga
     4321 acaccgtcat cggccagccg cccgaaccgg aaaggagctg caccgcgcac atgtagatct
     4381 cgtcgagatc gggccgctcc tcccggtcca ccttgacgca gacgaacgac tcgttgagga
     4441 gcgccgcgat cccggcatcc tcgaacgact cgtgctccat cacgtggcac cagtgacagg
     4501 cggcgtagcc gatcgacagg aagatcggct tgtcctcgcg ccgggcccgc tcgagcgctt
     4561 cctcacccca tgggtaccag tccaccggat tggcggcgtg ctggaggaga taggggctgg
     4621 tggccgaggc cagccggttc cggcgccctc ccgccgcggg gcccgtcatc ccgcttccac
     4681 cctcatcacg cccgccggac cgtctgcgcg atgttcgaac gcgcggcgtt cagcgcggga
     4741 aagaagccgc cgatgaggcc catgacgagc gcaaagagga aaccgatcac cagcatcacg
     4801 ggcgtcaccg tgaaccggaa ggccacctcg ctgaacgtgg cccagttggt ggtgccggtg
     4861 gagatgccgt tgatgggaag ggcgaggaat cccccgagga tgccgccgat cgacgacagg
     4921 agcagcgcct cgagcacgaa cgaaaggagc acgctgctac gcgaaaagcc gagcgccatc
     4981 atcgtgccga tttccgagcg ccggctgctg accgcggcgt acatggtgtt cagcgcgccg
     5041 aaaacggcgc cgaatgccat gatgatggaa atgaacatgc cgagcccctg gatcatgccg
     5101 gacacctgca tggactggtc ggcgtaatac tggctctcgc gcttcacgat ggcctgcatc
     5161 cggcgatccc cttcaacccg ggcctccagc gcctcgaact gggcggcgtc cttcagccgg
     5221 agcgaggccg actggtaccc ctcccgatcg aaggccggac caaggatctc gctgtcaccc
     5281 cagacttctg attcgaacga cgtgccgtcg gcatcgagga tgccgaccac ggtccaggcg
     5341 cggccgccga aggtgatctg ttcgccgagt tcgcatccct ggaatcgttc cgagatcagt
     5401 ttgccgacca cgacttcgct ctttccgctc tcccagaggc ggccggccat gagcttcagt
     5461 cgtggccgca gggccagggc ccgcggattg gtgccgcgcg tgaggatgtt ggcgatgccg
     5521 ccggtgcttt tctttttcag gttgaccacc accacgagct cgggaacgac catcggccgt
     5581 ccgtccgaat cgaccgccac ttccggctgc gtctcgatga tcgacaccca ttcccggctg
     5641 accgagctcg acagctcggc atcccccggc acccggagca cgatggcgtt gttgtcgagg
     5701 ccgtttccgg ccagcgcgcg ctggaatccc tgcgccaggg ccaggacggc gatgaacacc
     5761 gccacgacca gcgcgattcc gatgaccgtg atcgacgtgg ccagccggcg cacgaacagg
     5821 ttgcggaggt tgtaggagat cgggatcgcc actacacacc ctccctgagc gcgcgcgaaa
     5881 tgggcatccg ggcagcctgc agcgccggaa tcaacccgga aaggacgccc atgcccagcg
     5941 agaggaggag gccgtagccg atcgtggccg gcttcacgag gaacgagggg acgaatccca
     6001 tcaggtcgaa gccggtggcc cagtagagga aggtggcgag cccgatgccg agcgccccgc
     6061 cgatgagcga gatgaacgtg gcctccgcca cgatcaggcc caggatcgag ccgtcgctga
     6121 agccgagcgt cttcatcacg gcgatctcct tcgtccgctc acgcgccgcc atcatcatcg
     6181 tgttgaaggt gacgagggcg atggcgaaga caatggccgc cccgatgatc gagacgagga
     6241 atccgatgtt cccgatcatg ctcaggaagc cggcctggaa agccgcttcg gtttcggtct
     6301 tggtctcgta ggggctgttc gcgaagaggc cgtcgatcct gtcgcccacg acggccgtct
     6361 gccccgagtc ggccagcttg acgatataga agccgacgat tcccggatcg cccatcgtct
     6421 ggttcaggta atcccagttg aagtacatgc tgaggtcgtc cacgtccggc gtctcgggcg
     6481 tgtagatgcc gcggaccgtg aagcgccact cgtttccgtc gggataggga tagatcgttc
     6541 ccacgatcgg gagcgtgtcg ccgaccttga agccgtattt ctttgccagg cccgaaccga
     6601 ccacgcaccc ggtgcgatcg gccttgaatg ccgctaattg ctcgggcggc aggaggagtt
     6661 ccgggtacat gtcgaagtac gtgtcgacgt ccacgccgaa cttcgcaaag aacttctggg
     6721 ggtcctggta gatccccccg aaccagtttc cccacgacac ggcggtgacc ccgttcaccc
     6781 cggcgatgcg atcgcgatag gcaacgggca gcgggaagac cagcgaggtg ctccgtcgca
     6841 cgatcagccg cgtatcgtcc gccacctcga tcgcggcgtt gaacgccgtg aggaccgtcc
     6901 gaagggtggc aaatagaaac agcgccacgg cgatcgacag gatcgtgaga atggtccgcc
     6961 gcctgctgtg cgtcatgttc cggaagatga gccccaggaa cttcatgcgc ggaccgtcgc
     7021 ttccatgacg cccatgaccc ccttctcgaa ggtgcggacc tggtgggcac gctgggcggc
     7081 cgtgcggtcg tgcgtcacca tgatgatggt cttctggaat tccttgttca ggcgctccag
     7141 cagcgtgagg atttcatccg ccgagcgggc atcgaggtct ccggtcggtt cgtcggccac
     7201 gatgagcgtg gggtcggtca cgatggcgcg ggcgatggcc acccgctgct cctgtccgcc
     7261 cgagagctgc ttcgggtagt gcttcatccg gtcttccagg tccacgaggc ggagagccgt
     7321 catcacgtgg tcgtgccgct gggacctgga cagcggcgtc aggagcagcg gcagctccac
     7381 gttgtggtac gcgtcgagca ccgggatcag gttgtagaac tggaagatga agccgacgtg
     7441 ccgggcgcgc caggccgcca gcttcgaacc ggacatcgcg ctgatctcct cgccctccac
     7501 cacgatgc
//
LOCUS       DASVOD010000340        10060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_76364, whole genome shotgun sequence.
ACCESSION   DASVOD010000340 DASVOD010000000
VERSION     DASVOD010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76364"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VF720_07670"
     CDS             complement(<1..1332)
                     /locus_tag="VF720_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7879273.1"
                     /translation="MTLAAGARLGPYEIVAPLGAGGMGEVYRARDTRLGRDVAVKVLP
                     AHLADSPEGRARFEREARAISSLNHPHICTLFDVGREGDTDYLVMELVDGETLQKRLE
                     RGPLSSDEVLTLGRQIADALDKAHRQNMVHRDLKPANVMLTRTGAKLLDFGLARATGL
                     ATQVSDLTSSPTLTRALTTEGAIVGTFQYMSPEQLEGQEADARSDIWALGVTMYEAIT
                     GKRAFDGRSQASLIGAIMNNEPPPITTVSTLAPPALERLIKGCLTKDPEERIQTAHDV
                     KLQLQWIQEGGSQAGIPAPVAARRRSRERWLVVATTASTLAAVALLAWKLTTPRAPTP
                     VYRFEIPPPADVRFMDAPRISPDGSMIAYNAADSVGVMRIYVRPLNSLAARPLGGTEG
                     TQRPFWSPDSRFLGFITGGKLKKVDVTGAPPIVVCDATTGADGSWSRSGDIL"
     gene            1410..1583
                     /locus_tag="VF720_07675"
     CDS             1410..1583
                     /locus_tag="VF720_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879274.1"
                     /translation="MKHARLALGAHVVLSLIAASASFAASMVLPFTDDYEKALAEARS
                     KRLPLFIEAWAPW"
     gene            1608..2825
                     /locus_tag="VF720_07680"
     CDS             1608..2825
                     /locus_tag="VF720_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEX7879275.1"
                     /translation="MVFTDPALSSEAGRFVWLEVDTERPTSAAILKKYPLRALPTYFI
                     IEPDSERIAIRWVGGATVPGLKKILNDGDSRVRAAWTNPGLLAGDGGGGTPGAAGGSS
                     TGDSPMAGLLDEFLSRADQLYAAEEDSAAAVAYLTAIEMAPDDWPSYGRAVESAMFSL
                     SNTGDHEKTLALAKDAWPRLSRTSSAATVATSGLDAAANLPEGTEGKKALVDQFEAAA
                     RSVLTDPDIPATGDDRSGVYITLIGAHETLGDSAGAVALTREWADFLDKAAAEAKTPE
                     ARTVYDSHRLSAYLELKEPEKAVPFLLQSQKDFPEDYNPPARLAAAYKAMNDLPKALE
                     ANSRALALVYGPRSLRVYDARSDIQLAMGDNAAARATLEKALQTAEGMPDGQRSPRMI
                     ASFRKKLEGLPKT"
     gene            complement(2924..4039)
                     /locus_tag="VF720_07685"
     CDS             complement(2924..4039)
                     /locus_tag="VF720_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX7879276.1"
                     /translation="MSEPPGAAPPTEETTDVLIVGAGPIGLELAIALKGADVPYLQVD
                     AGPIGHTITGYPRQARFFSSPERIAIAGVPLVTIDQAKASREEYLAYLRQVVEQFDLP
                     VRTFERVTAIEVAGGADAAEPGPGRFRVTTQPAGGTRRYRARHVVLAVGDMAETRRLD
                     IPGEDLPHVSHLFDEPHRYFRKRLMIVGGKNSAVEAALRCHRAGAQVAISYRGERLPE
                     KSVKYWLLPEIESFIRQGHIGFHPGTVPVAIDPTGITLAPVVEPHGAADLNGKQVRLS
                     ADFVLLLIGYRADTSLFEMAGVTLEGENRGPRHDPETMETDIPGLFVAGTAAAGTQHH
                     FKLFIENSHVHVDRIVSRLTGCPMPQRVTRGFSLPES"
     gene            4085..4534
                     /locus_tag="VF720_07690"
     CDS             4085..4534
                     /locus_tag="VF720_07690"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX7879277.1"
                     /translation="MSPKRYRHDFPIRIHDVDAAGIVFFARYYVLMHDVYESFLADIG
                     FSISDVIRQGEVIIPVVESHCRYRRPMRHGETITGDIVLAELTATSYVVRCRLLGADD
                     ELKAVVSVRHVAVSQATMKPVPLPLSIREALAPYLAQDEPGLPGAPR"
     gene            complement(4710..5210)
                     /gene="tadA"
                     /locus_tag="VF720_07695"
     CDS             complement(4710..5210)
                     /gene="tadA"
                     /locus_tag="VF720_07695"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX7879278.1"
                     /translation="MSETPNASQLIDERWMREAMIEAERAAEAGEVPVGAVIVHDGRI
                     VGRGRNQMEGLADPTAHAEILAVTAAANTLKSWRLDGTTLYVTLEPCVMCIGAIFNAR
                     VSRLVFGALDPKRGAVGSVIDLSEEERLNHHLEVSSGPLHEESSAILKSFFEKLRRSA
                     KAGDKT"
     gene            complement(5224..5976)
                     /locus_tag="VF720_07700"
     CDS             complement(5224..5976)
                     /locus_tag="VF720_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879279.1"
                     /translation="MNRLTRAAVALFALALLAGPALAGKEKSTLKATVDPAYWDRKIT
                     KIGLLGFTWPHQDDVRMQETIPDLVKGALQDQGEFTLLFPDDVKKAAELGGNKEAYET
                     LMRVWRTRNELDQPALATIQAALGLDAIAGTEVTHWEQHKLDPSEEGYSTTTVGMRTR
                     MWDAHDRLLLWEATMVKVEKSPPYSPSTAVDAGGTTRSTGSVPEPPEYDVVTEEVVNQ
                     IIGSFPKEANKEQYMKAAAKKEKGSKKKEAGR"
     gene            complement(6128..6204)
                     /locus_tag="VF720_07705"
     tRNA            complement(6128..6204)
                     /locus_tag="VF720_07705"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6168..6170),aa:Arg,seq:acg)
     gene            6365..6865
                     /locus_tag="VF720_07710"
     CDS             6365..6865
                     /locus_tag="VF720_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEX7879280.1"
                     /translation="MNTNARTSHDPGRRRALLARLIADGQYARQEELVAALARHGMKV
                     TQATLSRDLVSLGVGKTPGRSGRTIYAIATPAAEVVDRRRQELDLTAFINEIKLAGQL
                     VVVRTPPGHAHGVARAIDLLGNPDVVGSIAGDDTILVVAPSPARARAVRRALETLGTN
                     GAPSTR"
     gene            6862..7893
                     /gene="argC"
                     /locus_tag="VF720_07715"
     CDS             6862..7893
                     /gene="argC"
                     /locus_tag="VF720_07715"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX7879281.1"
                     /translation="MSAPLVARVVLLGASGYAGLEFIKLAQRHDGITLTALASREAAG
                     RPARELSPVLAVAAGAASLPDIVTPEAGLAALVNGTADTLVSALPHGALADMMMAEPA
                     WLEAPRRIVDLSGDRRDGSLGFVYGLPEMNRERLTGAARVANPGCYPTAATLAVLPAV
                     EAGLASGPFSVTALSGVTGAGRKPALKTSFAELDGGAAFYQVGSTHAHVVEMQRNLTR
                     SGADITPRVAFAPQLVPMSRGILATVTAPLARPMSDADLRDLYAARYRDEPFVTLLAA
                     GEWPATHALRSTNMVTVSVTAVHDGMTLMAVAAIDNLVKGAAGQAVQNLNLMTGHPER
                     AGLTETSPW"
     gene            7887..9950
                     /gene="argB"
                     /locus_tag="VF720_07720"
     CDS             7887..9950
                     /gene="argB"
                     /locus_tag="VF720_07720"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX7879282.1"
                     /translation="MVTRITGTTSGPATGPVVIKLGGRALEGPGRLADFAADLVAHDR
                     PFVLVHGGGAEVTDWCRKLNIPSQFHEGLRVTDEASLEVATAVLTGIVSSRLVAGLVA
                     GGVPAVGLSGVDGGLLEAEPHPDADRLGRVGRVASVDTAWLGEILAAGRVPVLAPVAA
                     DAAGILYNINADDAAAAVAQALGASLLVFLSDVPSVVVDGRTVGTIGVAEATDILDHK
                     DVTGGMRPKLSAALNALGNGVGTAVIGAWSARGDLESLLATPAAGSAVNPTSPVEADL
                     FAGVYPFPRLELVHGRGCHVTARDGRDYLDFVSGIAVNALGHADPGLRNAVSRQMAQL
                     VHVSNLYGNGPAAALAARLVNLTGYDKVFLCNSGAEGNEAALKFARLHAGAKGHQDGI
                     IVAFDGAFHGRTAFALSATATPAYREPFGRLVPGIRFAPFNDAAAFDALVAEEAAAGR
                     PLQAVLIEPVQGEAGARTADPAFLAHLRAATRSANALLLFDEVQCGVGRSGSFLAAQE
                     LGIDADITILAKGLGGGIPIGAVLIRNDLAGLLSAGQHGTTFGGNPVAAAASGHVLDV
                     ITGPGFLTAVNERAVQLGAGLERLVAAHTSLKRVNGLGLLRAIELKEDAPFDPAALVL
                     AARNAGLLLCKGGSRSVRVLPPLVVAAADIDLALERLDAALTHLESEHLSNPTLALAG
                     KGERS"
     gene            9947..>10060
                     /locus_tag="VF720_07725"
     CDS             9947..>10060
                     /locus_tag="VF720_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017144038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEX7879283.1"
                     /translation="MTTQPYRKVALAYSGGLDTSIIVPWLKEKYGCQVVAYC"
BASE COUNT         1510 a         3527 c         3390 g         1633 t
ORIGIN      
        1 caggatgtcg ccgctccggc tccaggagcc gtccgcgccg gtcgtggcat cgcacaccac
       61 gatggggggc gcgccggtca cgtccacctt cttgagtttg ccgccggtga tgaagccgag
      121 gaagcggctg tccggcgacc agaaggggcg ctgcgttcct tcggtgcccc ccaggggtcg
      181 ggcggccagc gagttgaggg gcctcacgta gatgcgcatc acgcccaccg agtcggcggc
      241 gttgtaggcg atcatgctgc cgtccggcga gatgcggggc gcgtccatga agcggacgtc
      301 ggccggcggc gggatctcga accggtatac cggcgtgggg gccctgggcg tggtgagctt
      361 ccaggccagg agcgccacgg cggcaagcgt ggaggccgtg gtggccacga ccagccagcg
      421 ctcgcgcgaa cggcgccgcg ccgccacggg cgccgggatg ccggcctgcg agcccccttc
      481 ctggatccac tggagctgca gcttcacgtc gtgcgcggtc tggatccgct cctcgggatc
      541 cttggtcagg catcccttga tcaggcgctc gagcgccggc ggcgccagcg tggacaccgt
      601 ggtgatcggc ggcggctcgt tgttcatgat cgcgccgatg agcgaggcct ggctccgacc
      661 gtcgaaagcg cgcttgccgg tgatcgcctc gtacatcgtg acgccgagcg cccagatgtc
      721 gctgcgcgcg tcggcttcct gcccctcgag ctgctcggga gacatgtact ggaaggtgcc
      781 gacgatggcc ccttcggtcg tgagcgcgcg ggtgagcgtg ggcgaactgg tcaggtcgct
      841 cacctgggtg gcgaggccgg tggcccgcgc gaggccaaag tcgaggagct tggcgccggt
      901 cctggtgagc atcacgttgg cgggtttcag gtctcggtgc accatgttct ggcgatgcgc
      961 cttgtcgagg gcatccgcga tctgccggcc gagcgtgagc acctcgtccg acgagagcgg
     1021 cccgcgctcg agccgcttct ggagggtctc gccgtcgacc agctccatga cgaggtagtc
     1081 ggtgtcccct tcacggccca catcgaagag cgtacagatg tggggatggt tgaggctcga
     1141 gatggccctg gcctcgcgct cgaaacgggc gcgtccttcc ggggaatcgg ccaggtgagc
     1201 cgggaggacc ttcacggcca cgtcccggcc gaggcgggtg tcgcgggctc ggtacacctc
     1261 gcccatgccg ccggcgccca ggggcgcgac gatttcgtag ggcccgagac gggcccctgc
     1321 agccagagtc atggcgggag ttgtaaccgt tatcacttcc gaggtaaagc gcgtgccccg
     1381 gcacctgtgc gtctgttagc atcgggaaca tgaaacatgc gcgcctggcc ctcggggccc
     1441 acgtcgtgct gtcgctgatc gccgcctccg cgtcgttcgc ggccagcatg gtcctgccgt
     1501 tcaccgacga ttacgaaaag gccctcgcgg aggctcgcag caagcggctg ccgctgttca
     1561 tcgaagcctg ggccccctgg tgacactcgt gccgctcgat gcgggccgtg gtcttcaccg
     1621 accccgccct ctcgagtgaa gccggacggt tcgtctggct cgaagtcgac accgagcgcc
     1681 ccaccagcgc cgccatcctc aagaagtatc cgctgcgcgc gctgccgacc tatttcatca
     1741 tcgaacccga cagcgaacgc atcgccatcc gctgggtggg tggggccacc gtgccggggc
     1801 tgaagaagat tctcaatgat ggcgactcgc gcgttcgcgc ggcctggacc aatcccggcc
     1861 tcctggcggg cgatggcggc ggtggaaccc ccggcgcggc cggcggatcg tcgaccggcg
     1921 acagccccat ggccggcctc ctcgacgaat tcctctcccg cgcggatcag ctctacgcgg
     1981 ccgaggagga ttcggcggcg gcggtcgcct atctcacggc catcgagatg gcgccggatg
     2041 actggccctc ctacgggcgc gccgtcgagt cagccatgtt ctccctctcc aacaccggcg
     2101 atcacgagaa gaccctcgcc ctggcgaagg atgcctggcc gcgactgtca cggacgtcgt
     2161 cggcggctac ggtagccacc agcggcctcg atgcggccgc caatctcccc gagggaaccg
     2221 aggggaagaa ggcgctcgtc gaccagttcg aggcggcggc gcgcagcgtg ctcaccgatc
     2281 ccgacatccc cgccaccggt gacgaccgtt cgggcgtcta catcacgttg atcggcgcgc
     2341 acgaaacgct tggcgattcg gcaggggccg tggcactgac acgcgaatgg gccgacttcc
     2401 tcgacaaggc ggcggccgaa gcgaaaacgc cggaagcccg caccgtctac gattcacacc
     2461 ggctctcggc ctatctcgag ctcaaggagc ccgagaaagc ggtgccgttc ctgctccagt
     2521 cgcagaagga tttccccgag gactacaacc cgccggcccg actcgcggcc gcttacaagg
     2581 cgatgaacga cctgccgaaa gccctcgagg ccaactcgcg ggcgctggcg ctcgtgtacg
     2641 gtcctcgttc gctccgcgtg tacgacgccc gctcggatat ccagcttgcc atgggcgaca
     2701 acgccgcggc acgcgccacg ctcgagaagg ccctgcagac agccgaggga atgcccgatg
     2761 gacagcgttc cccgcgaatg atcgccagtt tcaggaagaa gctcgagggg ctgccgaaga
     2821 cgtaggcgct tcgacattct gtactcggtc ggcgcgtcgc gccgacatcc gcatgacggc
     2881 gggcggtccg gagttctccg ggccgtccgt ttcgctttcc tgctcagctc tccggaaggc
     2941 tgaacccccg tgtgacgcgc tggggcatgg gacacccggt cagccggctc acgatacgat
     3001 cgacatgcac gtggctgttc tcgatgaaga gcttgaaatg atgctgcgtg cccgccgcgg
     3061 cggtgccggc cacgaagagg ccggggatgt cggtttccat cgtctccggg tcgtgccggg
     3121 gtccacggtt ctccccctcc agcgtcacgc cggccatctc gaagagcgag gtgtcggcgc
     3181 gatagccgat caggaggagg acgaaatccg cggacagtcg gacctgtttg ccattcaggt
     3241 ccgccgcacc gtgcggctcg acgaccggcg ccagcgtgat gccggtcggg tcgatcgcca
     3301 ccggcacggt tccgggatga aacccgatgt gaccctgccg gatgaatgac tcgatctccg
     3361 gcagcagcca gtacttcacc gacttctcgg ggagcctctc gccgcggtag ctgatcgcaa
     3421 cctgcgcgcc ggcgcgatgg cagcgaagcg ccgcctcgac ggcggagttc ttccccccca
     3481 cgatcatgag acgcttccgg aaatagcggt gcggttcgtc gaacaggtgg ctcacatggg
     3541 gaaggtcctc gcccggaatg tcgagccgtc gtgtctccgc catgtcgccc acggccagca
     3601 cgacatggcg ggcgcggtaa cggcgggtgc cgccggcggg ctgggtcgtc acccggaagc
     3661 ggcccggccc cggctctgcg gcatcggctc ccccggccac ctcgatggcg gtcacccgct
     3721 cgaacgtccg caccgggagg tcgaactgct ccaccacctg ccggagatac gccaggtact
     3781 cctcgcggct ggccttggcc tggtcaatgg ttacgagcgg tacccccgcg atcgcgatcc
     3841 gttcgggaga ggaaaagaag cgcgcctggc gcggatagcc ggtgatggtg tggccgatcg
     3901 gaccggcgtc cacctggagg tagggaacgt ccgcgccttt cagcgcgatg gccagctcga
     3961 ggccgatcgg tccggcgccg acaatcagga cgtccgtcgt ttcttcggtc ggcggcgcgg
     4021 cgccgggggg ttcgctcatg gcgttgaagg ctcgttcatg cggctatggt aacctgagga
     4081 ccccatgtcg ccgaagcgct accgccacga cttccccatc cgcatccacg acgtggacgc
     4141 cgccggcatc gtcttcttcg cgcgttacta cgtcctgatg catgacgtgt acgagtcgtt
     4201 cctggccgac atcggattct ccatctcgga cgtcatccgc cagggggagg tgatcatccc
     4261 ggtcgtcgaa agccactgcc gctatcgccg cccaatgcgg cacggcgaga cgatcaccgg
     4321 tgacatcgtg ctggccgaac tgaccgccac gtcgtacgtt gtgcggtgcc ggctgctggg
     4381 tgcggatgat gaactcaagg ccgtggtcag cgtgcgccac gtggcggtct cgcaggccac
     4441 catgaagccg gtgccgttgc cgctctccat acgcgaggcc ctggcgccct acctcgccca
     4501 ggacgaaccc gggctgcccg gcgcgccgcg ctgacggcac gcgcccgtcg caacgacgtc
     4561 cattcctctc tcagagagcc tgttccggaa caggtaacgc gctggcgcgc acatcagcgc
     4621 ccgcgactct cccaggaacc tgttccggaa caggtagcgc agcgggcccg cagcggccca
     4681 cgaccctatc tgccaaacag gctggcgccc tacgtcttgt cccccgcctt ggcactccgc
     4741 cgcaacttct cgaagaacga cttcaggatg gccgacgact cctcatgcag cggccccgac
     4801 gaaacctcga ggtggtgatt cagccgctcc tcctcggaaa ggtcgatgac cgaccccacc
     4861 gcgccccgct tgggatccag cgcgccgaag accaggcgcg agacgcgggc gttgaagatg
     4921 gcgccgatgc acatgacgca tggctcgagc gtgacgtaga gcgtggtgcc gtcgagccgc
     4981 cacgacttga gcgtgttggc ggcggccgtc accgccagga tctcggcgtg ggcggtggga
     5041 tcggcgaggc cttccatctg gttgcggccc cgaccgacga tgcggccgtc gtggacgatc
     5101 acggcgccga ccgggacttc gccggcctcc gcggctcgtt cggcttcgat catggcctcg
     5161 cgcatccagc gctcgtcgat cagctgggac gcattgggag tttcgctcat cgggagaagg
     5221 cggtcagcgc ccggcctcct tcttcttcga gcccttctcc ttcttggcgg cggccttcat
     5281 gtactgctcc ttgttcgctt ccttcgggaa ggagccgatg atctggttca cgacttcttc
     5341 ggtcaccacg tcgtattcgg gaggttcggg gacgctcccg gtggagcggg tggtgccgcc
     5401 ggcgtcgacg gctgtcgagg ggctgtaggg gggcgacttc tccaccttga ccatcgtggc
     5461 ctcccagagg aggaggcgat catgcgcgtc ccacatccgg gtgcgcattc cgacggtagt
     5521 cgtactgtac ccctcctcgc tcgggtcgag cttgtgctgc tcccagtggg tcacttcggt
     5581 cccggcaatg gcgtcgaggc cgagcgccgc ctggatggtg gcgagggccg gctggtcgag
     5641 ctcgttgcgg gtgcgccaga cccgcatgag cgtttcgtag gcctccttgt tgccgcccag
     5701 ttccgcggcc ttcttcacgt cgtccggaaa gaggagcgtg aattccccct ggtcttgcag
     5761 tgcccccttc acgaggtcgg ggatcgtctc ctgcatgcgg acgtcgtcct ggtggggcca
     5821 ggtgaagccg aggagcccga tcttggtgat cttgcgatcc cagtaggccg ggtcgaccgt
     5881 ggccttgagg gtgctttttt ccttgccggc cagtgccggg ccggccagaa gggccagggc
     5941 aaacagcgca accgccgctc gggtcaggcg attcatcgtg gggatcctcc gggacgccgg
     6001 actcggccgg ccgagatacc aacaggtacg cgcgacatgc acgcgacaac cggggaaagt
     6061 ctagggggat tcgcgcggag cgcgcaaccg ttgcgtaaac cgggagagga ggtgccgcgg
     6121 agggaagtgg tgcgcccagg aggagttgaa cccccaacct tctgatccgt agtcagacgc
     6181 tctatccaat tgagctatgg gcgcgacctt ctgtcgccgg agggtgcaac gcacccgatc
     6241 gacattgaca ggcggggaat cttagggaga agcccgacag atttcaagcc cgaatccccc
     6301 acccacgacc tgctgcgtga aaacagttga aaccgcggcc ccgtttgtgt ttttattcaa
     6361 accaatgaat acaaatgcac gcacctccca cgaccccggc cggcgccggg ccctgctggc
     6421 ccgcctgatc gccgacggcc agtacgcccg ccaggaggaa ctggtagccg cgctggcccg
     6481 gcacggcatg aaggtcaccc aggccacgct ctcgcgagac ctggtgagcc tcggcgttgg
     6541 caagacaccc ggccgctccg gacggaccat ctacgccatc gccacgccgg ccgccgaagt
     6601 ggtcgatcgc cggcgccagg agctggacct caccgcgttc atcaacgaga tcaagctggc
     6661 cggccagctg gtggtggtgc gcacgccccc gggccatgcc cacggcgtag cccgggccat
     6721 tgacctcctc ggcaacccgg acgtggtcgg ctcgatcgcc ggcgacgaca ccatcctggt
     6781 cgtggctccc agcccggccc gcgcgcgggc cgtccgccgc gcgctcgaaa ccctcggcac
     6841 gaacggggcg ccctccaccc gatgagcgcg cctctcgtgg cgcgcgtggt cctcctcggc
     6901 gcctccggat atgccggcct cgagttcatc aagctggccc aacgtcacga cggcatcacg
     6961 ctcacggcgc ttgcctcgcg tgaggcggcc ggtcgtcccg cgcgggagtt gtcgccggtg
     7021 ctggcggttg ccgccggagc ggcatcgctt cccgacatcg tcacgcccga agcgggcctg
     7081 gcggcgctgg tcaacggcac tgccgatacg ctggtaagcg cccttccgca tggcgcgctc
     7141 gccgacatga tgatggcgga gcccgcctgg ctcgaagcac cgcgccggat cgtcgacctt
     7201 tcgggcgatc ggcgcgatgg ttcgctcggc ttcgtctacg ggctccccga aatgaaccgc
     7261 gagcgcctga ccggtgctgc gcgcgtggcg aacccggggt gctacccgac cgccgccacg
     7321 ctggccgtgt tgcccgccgt cgaagcggga cttgcctcgg gaccgttcag cgtgacggcg
     7381 ctctcgggcg tgaccggcgc cggacggaag cccgcgctca agacctcgtt cgccgaactc
     7441 gacggcggcg ccgccttcta ccaggtggga tcgacccatg cccacgtcgt cgagatgcaa
     7501 cgcaacctga cgcgctccgg cgccgacatc accccccggg tggcgttcgc cccgcagctc
     7561 gtgcccatgt cacgcggcat cctggccacc gtcaccgcgc ctcttgcgcg gccaatgtcc
     7621 gatgccgacc tgcgcgatct ctacgccgcc cgctaccggg acgagccgtt cgtcacgctt
     7681 cttgcagcgg gtgaatggcc cgccacgcac gccctgcgca gcacgaacat ggtcaccgtc
     7741 tcggtcaccg ccgtccacga cggcatgacg ctcatggcgg tggccgccat cgacaacctc
     7801 gtgaagggtg cggccggcca ggccgtgcag aacctgaacc tcatgaccgg ccaccccgag
     7861 agggccgggc tgacggagac atcgccatgg tgacgcgcat cacgggaacg acctccggcc
     7921 cggccacagg accggtcgtg atcaagctcg gcggtcgtgc gctggagggg ccggggcggc
     7981 tcgcggattt cgcggccgac ctggtcgcac acgaccgccc cttcgtgctc gtccacggcg
     8041 gtggtgccga agtcaccgac tggtgccgga agctcaatat tcccagtcag ttccacgaag
     8101 ggcttcgcgt gacggatgag gcctccctcg aagtggccac ggccgtcctc accggcatcg
     8161 tcagcagtcg cctcgtcgcg gggctggtag ccggtggcgt gccggcggtc gggttgtccg
     8221 gcgtcgatgg cggcctcctc gaggccgagc cccacccgga cgccgaccgg cttggccgtg
     8281 tcggacgggt agcctccgtc gatactgcct ggctcgggga gatcctcgcg gccggtcgtg
     8341 tgccggtcct ggccccggtg gcggcggatg ccgcgggcat cctctacaac atcaacgcgg
     8401 atgacgcggc cgccgcggtg gcgcaggcgc tcggcgcgtc cttgctggtc ttcctgtccg
     8461 atgtcccgtc ggtcgtggtc gatggcagga cggtcggtac gatcggcgtg gccgaggcca
     8521 ccgacatcct cgatcacaag gacgtcaccg gcggcatgcg gccgaagctg tcggcggcgc
     8581 tcaacgctct gggcaacggc gtcggtacgg ccgtcatcgg cgcctggtcg gcgagaggtg
     8641 acctcgagtc actgctggca acacccgccg cggggagcgc ggtgaatccg acctccccgg
     8701 tagaagccga cctcttcgcc ggcgtctacc ccttcccccg cctcgaactc gttcacggcc
     8761 gggggtgcca cgtcaccgca cgtgacgggc gcgactacct cgacttcgtg agcggcatcg
     8821 ccgtgaatgc gctcgggcac gccgacccgg gtcttcgcaa cgcggtgagc cggcagatgg
     8881 cacagctcgt ccacgtctcg aacctgtacg gcaacgggcc ggcggcggcg ctggcggcgc
     8941 ggctcgtcaa cctgaccggc tacgacaagg tattcctgtg caactcgggc gccgaaggca
     9001 acgaggcggc cctcaagttc gcgcgcctcc atgcgggggc caaaggccac caggatggga
     9061 tcatcgtggc cttcgacggc gccttccacg gccgcacggc ctttgcgctg tcggccacgg
     9121 ccacgcccgc ctaccgcgag ccgttcggga ggctggtgcc gggcatccgc ttcgcgccct
     9181 tcaacgacgc cgccgccttc gacgcgctgg tggccgagga agccgccgcc ggccggccgc
     9241 tccaggccgt gctcatcgag ccggtacagg gggaagccgg tgcgcgcacg gccgacccgg
     9301 ccttcctggc ccatctccgg gctgccacgc gctcagccaa cgcgctcctc cttttcgatg
     9361 aggtgcagtg cggggtgggg cgatcgggct cgttcctggc tgcccaggag ctcggcatcg
     9421 acgcggacat caccatcctg gccaaggggc tcggcggcgg cattccgatc ggcgccgtgc
     9481 tcatccgcaa cgacctggcc gggttgctct ccgccggcca gcacggcacc acgttcggcg
     9541 gcaacccggt ggccgcggcc gcgtcgggac atgtcctcga cgtgatcacg gggcctggtt
     9601 tcctcaccgc cgtgaacgag cgcgccgtgc agctcggcgc cggcctcgag cggctggtcg
     9661 cggcccatac gtccctgaag cgcgtgaacg gcctcggact gctccgggcc atcgaactga
     9721 aagaagacgc gccgttcgat ccggccgcgc tcgtgctcgc cgcacgcaac gccggcctcc
     9781 tcctctgcaa gggaggctcg cgcagcgtgc gcgtccttcc gccgctcgtc gtggccgcgg
     9841 ccgacatcga cctggccctc gagcggctcg acgccgcgct cacgcatctc gaatccgaac
     9901 acctctcgaa tccaacgctg gcactcgccg ggaaaggaga acgctcatga ccacccagcc
     9961 gtatcgcaag gtggccctcg cctattccgg gggcctcgac acttcgatca tcgttccctg
    10021 gctcaaggag aaatatggct gccaggtcgt ggcctactgc
//
LOCUS       DASVOD010000341         7913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_232286, whole genome shotgun sequence.
ACCESSION   DASVOD010000341 DASVOD010000000
VERSION     DASVOD010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232286"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VF720_07730"
     CDS             complement(<1..1075)
                     /locus_tag="VF720_07730"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX7879284.1"
                     /translation="MSAIRVNWDGGEAHVDAAPNDTVGQVLARAGAPDTLIGARADGN
                     LKGVMDPVGGAAELTGVTLESADGLFLLRHSSAHIMATAIQELYPGTKFAIGPPIADG
                     FYYDMQSSRAFTPEDLEAIEKKMRGIIAQKMPFQRIEANRLEAIDKFKASDDPYKVEI
                     LEGLPDDTVTYYVNGPFTDLCRGPHAPSTGSLNAKAFKLLQVAGAYWRGDEMRPMLQR
                     IYGTTWATPDDLAKYLDKLEQAKLRDHRKLGKELDLFSVHDDGGAGLIFWHPKGGRIR
                     KAVEDYWRDTHLKGGYEIVFTPHVGKSTLWETSGHLGFYRENMYAPLDVDGQEYFLKP
                     MNCPFHILIYQTAMRSYRDMPFRW"
     gene            complement(1166..1242)
                     /locus_tag="VF720_07735"
     tRNA            complement(1166..1242)
                     /locus_tag="VF720_07735"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1206..1208),aa:Val,seq:gac)
     gene            1370..2617
                     /locus_tag="VF720_07740"
     CDS             1370..2617
                     /locus_tag="VF720_07740"
                     /inference="COORDINATES: protein motif:HMM:NF018448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEX7879285.1"
                     /translation="MTAETDPRIVYLLAMARALHVSGYPAHRLESVLAATAARLGVTA
                     QFFSTPTSLFAAFGLESRQHTHLLRVEPGNVLLERLDRLHAVGASVQAGTRTPGEGTA
                     AIAAIMEAPPRWRGVVWLLAAAVSSACAARFLGGGHMEVTGAFAIGLVIAGLASAASR
                     RAALGRVLTPLAGCIGAMIAGVAAAWFSPASLPIMLVGGLIALVPGFVLTVATSELAA
                     GHLVSGTARLTGAIIQLIAMVIGVAIGLAIVTRFTGALPAPASSALPFATEWIALLVA
                     PIALGIVFQAAPANFGWIVLIALVGFLGGRLGASALGPELGLLVGTLSAGLVNGFLAR
                     ARGNQGTANMVPALIMLVPGSVGFRGLIELLNRNVVSGVELAFQTAIMTSSMSAGILL
                     ANALTPARGVEGTSVAVGPGEPV"
     gene            complement(2854..3195)
                     /locus_tag="VF720_07745"
     CDS             complement(2854..3195)
                     /locus_tag="VF720_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7879286.1"
                     /translation="MTDFLRNNGSDELERRRRLSMKAVVAGSIVGAGAALLLAPCSGR
                     DLRCKIREGSGKIGRSVGDRASHLAHDAARLGRKVGRAVSTAAADVKDSLRAGRNAMN
                     DVADGTTSYYP"
     gene            complement(3524..5185)
                     /locus_tag="VF720_07750"
     CDS             complement(3524..5185)
                     /locus_tag="VF720_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX7879287.1"
                     /translation="MTFKRFFRGLAAGLVLAAALGAAAVRPIGADEPAPDVVEIDIIY
                     TSDIHGHIDPQEATFLNPQFPPPLGGGASAATYIRQVKEAAAREGRGFLMVDSGDLFQ
                     GTPVGMHFQGKAVIEWMNEVGYTAAALGNHDFDLGWTITEELVRTAKFPILCANLYKT
                     GTNERVDWVQDMVITETAGVKTAIIGMVTEATAKISFAKNIDGLTFRPLDEMLPIKVE
                     EARAKGAQLVLFPVHAGLPYKPNLAEVYRDMIEEEQRGERPREFEAMELAHYIHGVDI
                     IFAGHSHQGYDVPWEDPVTHTLVLEPYANGSSLGHVTVKFDRRLGKMIGYDTHHDRGA
                     LVTLFEDEFWPDQGVAETIGERVAEAEKGLDETIGETRVNLQRGDADRGNLGALVADA
                     IREFTNSDLAVQNTGGVRADIAPGEITKRHALAVLPFGNTVVEATVKGEFIQRLFEQK
                     VGSYGPSLFISGATIEYDPTRPQGSRIHSITIGDKPLDPEATYTIAMTNFLSEGNSGM
                     TLMREVRTEDFRFTGNTDREALERYIQAHTPINPKGESRWVRVSS"
     gene            complement(5230..6561)
                     /locus_tag="VF720_07755"
     CDS             complement(5230..6561)
                     /locus_tag="VF720_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879288.1"
                     /translation="MRTVLRPEAMTMMISKQTGALALLALAVLGAAEPARAYTYLESA
                     WGTPVAAQSSRGLAMGGATVAVPDGAFSLVTNPAMMGSETERAFEMSMRASRYDETRY
                     VPLFDSFDSFIKETAITENPETYARVNGGVLWRPEKEDSKFAIGGGIYERFNFQYDFL
                     DERRVANGRDTVNRDKVQATQIIRSDASIYSLSAGAAYKEKTLGLGAALHYYFGEVGF
                     SNSIAPGPAPEPYREDASLDALNREMSGVGATFGASADIDERVTLAASYTLPVTFDTD
                     WRHETLADTTIGSDDVEYPGQLALGMALRPRNNPRTLFSLAAVRTFWQEDLVDPVLEA
                     NPVVDVAAGDVRNTWDFRAGVEHVFYNNLPARFGFIYRELYTASDADEAGVAFGVGYK
                     FDAWDIGFGMDVLKRNSRQDAITPRTNSEDPKTDRVTDSLVQGIVDIRYRF"
     gene            complement(6575..>7913)
                     /locus_tag="VF720_07760"
     CDS             complement(6575..>7913)
                     /locus_tag="VF720_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879289.1"
                     /translation="QDMLKPDYREHFLPMRDVMQETIVGGNVRFKFLPAAYVGVTAME
                     MTYRNNLYPDADSDSLVHRFNPDPNTIIIEPSRLEARDAEFAAGYDNRQLGNFRDLVG
                     TDFGLVVKNLSLAGEYGKLLTSPEESFTGRAFSSGPEAYLGSAYLQYENLNLQALYRD
                     YDLHYDNPYNRGFSETNKYDQTLAGDEFRDWNPIFSYLAENDPQSKAEKGFYLIGRYQ
                     LSRELTLNYFEYDNYVRKVDDTNSQRLTANFEYRPIFPVRFRVRQRWSERDGKGEDEV
                     RGFTGWDTRFEARFYLSDYNRLDFLYSTTNVIFKPRPRLGSDGDETGGTTDLGTRASP
                     GQAFQAKYTHNFTPGLAVTVSSEVYDGFLYNFEDNEFIAIDGTGFRNWFLVRSRLSTD
                     LSWRLKYTVDNQDPISYLQIRNFETPDPNGGRPQGSNVLDNVNSFRFQLDYTF"
BASE COUNT         1218 a         2554 c         2667 g         1474 t
ORIGIN      
        1 cccaccggaa cggcatgtcg cgataactgc gcatcgcggt ctggtagatc aggatgtgga
       61 acgggcagtt catcggcttg aggaagtact cctggccgtc cacgtcgagc ggcgcgtaca
      121 tgttctcgcg gtagaagccg agatgccccg aggtttccca cagggtggac ttgccgacgt
      181 gcggggtaaa cacgatctcg taaccaccct tcaggtgggt atcgcgccag tagtcctcca
      241 cggcctttcg gatccggccc cccttggggt gccagaagat caaccccgct cccccgtcgt
      301 cgtgcacgga gaagaggtcg agctccttgc cgagtttgcg atggtcgcgc agtttggcct
      361 gctcgagctt gtcgaggtat ttcgccaggt cgtccggcgt ggcccaggtg gtgccgtaga
      421 tgcgctgcag catcgggcgc atttcatcgc cccgccagta ggcgccggcc acctgaagca
      481 gcttgaacgc tttggcgttc aggctgccgg tggaaggggc gtggggaccg cggcagaggt
      541 cggtgaacgg gccattcacg taataggtga cggtgtcgtc cggaaggcct tcgaggatct
      601 cgaccttgta cggatcgtcc gacgccttga acttgtcgat ggcctccagg cgattggctt
      661 cgatccgctg gaacggcatc ttctgggcaa tgatgccgcg catcttcttt tcgatggctt
      721 cgaggtcttc gggcgtgaac gccctcgacg actgcatgtc gtaatagaac ccgtccgcga
      781 tcggcggccc gatggcgaac ttggtgccgg gatagagctc ctggatggcg gtggccatga
      841 tgtgcgcgct ggagtggcgg agcaggaaga gcccgtcggc cgattccagg gtcacgccgg
      901 tcagctcggc cgctcccccc acggggtcca tgaccccctt caggttgccg tccgcccggg
      961 cgccgatcag ggtgtcgggg gccccggccc gggccagcac ctgccccacc gtgtcgttgg
     1021 gggcggcatc gacatgggcc tcgcccccgt cccagttcac ccgaatggcg ctcatggcga
     1081 tgtcctcatg ccctttcggg gccccggaga aaggttcgac cggataaaac gaagcaggcc
     1141 atgcggcctg ctcggttcgt tcaagtggtg ggcgatactg gatttgaacc agtgacccct
     1201 tgcatgtcaa gcaagtactc taaccaactg agctaatcgc ccactcgaat tgcgagcggg
     1261 gattctgccg aagtcggcgg ggcgtgtcaa tcggcggccg ctcggacagg ccgcgctcgg
     1321 cccccacgcc cttccgaacc ggcgcgccag ccggtatcgt gccccaccca tgaccgccga
     1381 gaccgacccc cgcatcgtct acctcctggc gatggcgcgc gccctccacg tgagcggcta
     1441 tcccgcccac cggttggaat cagtgctggc cgccacggcc gcgcgactcg gggtcacggc
     1501 ccagttcttc agcacgccca cgagtctctt cgcggcgttc ggactcgaga gccgccagca
     1561 cacccacctc ctccgggtgg agccggggaa cgtgctcctc gagcggctcg accggcttca
     1621 tgcggtgggc gcctcggtcc aggccggcac gcggacgccg ggcgagggta cggccgccat
     1681 cgctgcaatc atggaagcgc cgccccgctg gcggggtgtg gtctggttgc tggcggccgc
     1741 ggtgagctcg gcctgcgcgg cacggttcct cggcggcgga cacatggagg tgaccggcgc
     1801 cttcgcgatc ggcctggtca tcgcgggact ggcatcggcg gcctcccgga gagccgcgct
     1861 ggggcgcgtg ctcacgccgc tggccggatg tatcggggcg atgatcgccg gcgtcgcggc
     1921 ggcatggttc tcgccggcat cgttgccgat catgctggtc ggcggcctca tcgccctcgt
     1981 gcccggcttc gtcctgacgg tggccaccag cgaactggcg gccggccatc tcgtgtccgg
     2041 aaccgcccgc ctgaccggcg ccatcatcca gttgatcgcc atggtgatcg gcgtggccat
     2101 cggcctggcg atcgtgacgc gcttcaccgg tgcgttgccg gccccggcct cctccgcgct
     2161 cccgttcgcc accgaatgga tcgcgctgct cgtggccccg atcgccctcg gcatcgtctt
     2221 ccaggccgcg ccggccaact tcggctggat cgtcctgatt gccctcgtcg gattcctcgg
     2281 cggtcgactc ggcgcctcgg cgctcggccc ggaattgggg ctcctggtcg gcaccctgtc
     2341 ggccggcctg gtgaacgggt tcctggcccg cgcccgcggc aaccagggca ccgcaaacat
     2401 ggtgccggcg ctgatcatgc tcgtgccggg gagcgtcggc ttccgcgggc tgatcgagct
     2461 gctcaacagg aatgtggttt cgggcgtcga actcgccttc cagacggcga tcatgacgtc
     2521 gtcgatgtcc gccgggatcc tgctggcaaa cgcgctgacc ccggcgcgcg gggtggaagg
     2581 gacgtccgtc gcggtcggtc cgggggagcc ggtgtgaggc gagttggagg gtgtgctcga
     2641 cctgagttgg cttcagggtc gaactctgcc tgaaccgagt tgccgaggct gattgccggt
     2701 cagggtcgaa ctctgcctga accgagtggc gccgggccag aattcgtaac aggttcgccg
     2761 gtgcaacgca gctggcgcgc atcgtcccga cgcacgaagg tcctcaccgc atcgtcgcgg
     2821 agaggacctt cgaactgcgg aaatctcacg tactcagggg taatagctgg tcgtgccgtc
     2881 cgccacgtcg ttcatcgcgt tgcggcccgc acggagcgag tcctttacat ccgccgccgc
     2941 ggtcgagacg gcccggccga ctttccggcc gagacgggcc gcgtcgtggg ccaggtgcga
     3001 ggcacgatca ccaaccgacc ggccgatctt ccccgatcct tccctgatct tgcagcgaag
     3061 gtcacgcccc gaacacggtg ccaggagaag cgcggccccg gcgccgacga tcgagccggc
     3121 cacgaccgct ttcatcgaga ggcggcgacg ccgctccagt tcatcactgc cgttgttgcg
     3181 caggaaatcc gtcatgacat acctccagtt cgatgaggcg ggagcgtcac ccctcggtga
     3241 cgggctcgcc ggaacctcgt ctcaccgtcc gccggcggcg ccggtgatgt ccggcgcccg
     3301 cggtggatgg cgtatccggt atatagaagg ttgcaagcgc tgggccaaag tgacgtgaaa
     3361 tcgatttaac tgatttgatt acagggcctt aaccggcttt ctttgcaccg cacatgaaaa
     3421 aactcccccc acggcggctg cgatgggggg agttcctacc ttataggcca ttacgttcct
     3481 gtccaccggc gcttccctcg gaccttcctt gcgccgtgat gcgctacgag ctgacccgca
     3541 cccaccgact ctcccctttc gggttgatcg gcgtgtgagc ctggatgtag cgctcgagcg
     3601 cttcgcgatc cgtattgccg gtgaaacgaa agtcctcggt ccgcacctcg cgcatcagcg
     3661 tcatgccgct gttcccctcg gagaggaagt tcgtcatggc gatggtgtag gtggcctccg
     3721 ggtcgagcgg cttgtcgccg atcgtgatcg agtggatccg gctcccctgc ggcctggtcg
     3781 ggtcgtactc gatggtggcg cccgagatga acagcgaggg gccgtagctc cccaccttct
     3841 gctcgaaaag gcgctggatg aactcaccct tcaccgtggc ttcgacgacg gtgttgccga
     3901 acggcaggac ggccagcgcg tgccgcttcg tgatctcgcc gggggcgatg tcggcccgga
     3961 cgccgccggt gttctggacg gccaggtcgg agttggtgaa ctcgcggatg gcgtcggcga
     4021 ccagcgctcc caggttgccg cgatcggcgt cgccgcgctg gagattgacg cgcgtttcgc
     4081 cgatcgtctc gtcgagcccc ttctcggcct cggccacgcg ttcgccgatc gtttcggcta
     4141 ccccctggtc gggccagaac tcatcttcga acagggtaac cagcgcgcct cgatcgtggt
     4201 gcgtgtcgta gccgatcatc ttccccagcc gccgatcgaa cttgaccgtg acgtggccga
     4261 ggctcgaccc gttggcgtac ggctccagta ccagcgtgtg cgtcaccggg tcttcccacg
     4321 gcacgtcgta accctggtgc gagtggccgg cgaagatgat gtccacgccg tggatgtagt
     4381 gcgcgagctc catcgcctcg aactccctgg gtcgctcgcc gcgttgctcc tcctcgatca
     4441 tgtcacggta gacttcggcc aggttcggct tgtagggaag gccggcgtgc accggaaaga
     4501 ggacgagctg ggcgcccttc gcgcgggcct cctcgacctt gatggggagc atctcgtcga
     4561 gcggccggaa cgtgagtccg tcgatgttct tggcaaacga gatcttggcg gtggcttcgg
     4621 tgaccatgcc gatgatggcc gtcttcacgc cggccgtctc cgtgatcacc atgtcctgaa
     4681 cccagtccac ccgctcgttc gtgccggtct tgtacaggtt ggcgcagagg atcgggaact
     4741 tggccgtccg gacgagctcc tcggtgatcg tccaccccag atcgaagtcg tggttgccga
     4801 gcgcggccgc cgtgtagccc acttcgttca tccactcgat gacggctttt ccctggaagt
     4861 gcatgccgac cggcgttccc tggaagagat cgcccgagtc gaccatgagg aagccgcgac
     4921 cctcccgggc cgccgcctcc tttacctgcc ggatgtaggt ggcggccgac gctccgccac
     4981 ccaggggagg cgggaactgc ggattgagga aggtggcttc ctgcggatcg atgtgtccgt
     5041 ggatgtcgct ggtgtagatg atgtcgatct cgaccacgtc gggcgcgggc tcgtcggccc
     5101 cgatggggcg gacggccgct gcgcccagtg ccgcggcgag aaccagtccc gcggcaaggc
     5161 cgcggaagaa tcgcttgaag gtcacgcgat cactctcctg tcagcggggc ggcccctgcg
     5221 gccccccacc tagaaacgat accggatgtc cacgatcccc tgcacgaggc tgtcggtaac
     5281 gcggtcggtc ttgggatctt cgctgttggt acgcggcgtg atggcgtcct gccgcgaatt
     5341 gcgcttcagg acgtccatgc cgaatccgat gtcccaggcg tcgaatttgt agccgactcc
     5401 gaaggccacg ccggcctcat cggcatccga agcggtgtac agctcccggt agatgaatcc
     5461 gaagcgggcc ggcagattat tgtagaacac atgttccacg cccgcgcgga aatcccaggt
     5521 gttgcgcacg tcgccggcgg cgacgtcgac caccgggttg gcctcgagga ccgggtcgac
     5581 caggtcttcc tgccagaagg tgcggaccgc cgccaggctg aacagggtgc gggggttgtt
     5641 gcgcggcctg agcgccattc cgagcgccag ctggccaggg tactccacgt cgtcgctgcc
     5701 gatcgtggtg tcggccagcg tctcgtgccg ccagtcggtg tcgaaggtga ccggcagcgt
     5761 gtaggaggcg gccagcgtca cgcgctcgtc gatgtcggcc gacgcgccga aggtggctcc
     5821 gacgccggac atctcccggt tcaacgcgtc gagcgaggca tcttcacgat acggctcggg
     5881 agccggtccg ggcgcaatgg aattgctgaa gccgacttcg ccgaagtagt agtgaagcgc
     5941 ggcgccaagg cccagggtct tctccttgta ggcggccccg gccgacagcg aatagatcga
     6001 ggcatccgag cggatgatct gggtggcctg caccttgtcg cggttgaccg tgtcgcggcc
     6061 gttggccacc cggcgctcgt ccaggaagtc gtactggaag ttgaagcgct cgtagatgcc
     6121 gcccccgatg gcaaacttcg agtcctcctt ctccggacgc cagaggacgc cgccgttgac
     6181 ccgggcatag gtttccgggt tctcggtgat ggcggtctcc ttgatgaacg aatcgaagga
     6241 gtcgaacagc ggtacatacc gcgtttcatc gtaacgggag gcgcgcatcg acatctcgaa
     6301 agcgcgctcg gtctcgcttc ccatcatggc cgggttggtc accagcgaaa acgcgccgtc
     6361 gggcaccgcc acggtggccc cgcccatggc gaggccgcgc gacgactggg ccgccaccgg
     6421 cgtcccccag gcggattcga ggtaggtgta ggcgcgggcg ggttccgccg cgccgaggac
     6481 ggccagcgcc agcagcgcca gcgcccccgt ctgcttcgag atcatcatcg tcatggcctc
     6541 cggtcggaga acggttctca tcgggtcgcc ctccctagaa ggtgtaatcg agctggaagc
     6601 ggaaggagtt cacattgtcg aggacgttcg acccctgtgg cctgccgcca tttggatccg
     6661 gggtttcgaa attgcggatc tggaggtacg agatcgggtc ctggttgtcg accgtgtact
     6721 tgagtcgcca ggagaggtcg gtcgacaggc ggctccgcac caggaaccag ttgcggaagc
     6781 cggtgccgtc gatcgcgatg aactcgttgt cctcgaaatt gtagaggaag ccgtcgtaca
     6841 cctcgctcga caccgtgacc gccaggcccg gcgtgaagtt gtgggtgtac ttggcctgga
     6901 acgcctggcc cggtgaagct cgcgtgccga ggtccgtggt tccgcccgtc tcgtcgccgt
     6961 ccgaaccgag gcggggccgc ggcttgaaga tgacgttggt ggtggaatag aggaagtcga
     7021 ggcggttgta atcggagagg tagaagcggg cctcgaatcg tgtatcccag ccggtgaagc
     7081 cgcggacctc gtcttccccc ttgccgtcgc gctcggacca gcgctggcgc acccggaacc
     7141 gcaccggaaa gatcgggcgg tactcgaagt tggccgtgag gcgctggctg ttggtgtcgt
     7201 cgaccttgcg gacatagttg tcgtactcga agtaattgag cgtgagctca cgcgacagct
     7261 ggtaacgacc gatcaggtag aaccccttct cggccttcga ctgcgggtcg ttctccgcca
     7321 ggtacgagaa gatcgggttc cagtcgcgga actcgtcgcc ggccagcgtc tggtcgtact
     7381 tgttggtttc ggagaagccg cggttgtacg ggttgtcgta gtgcaggtca tagtcgcggt
     7441 agagcgcctg caggttcagg ttctcgtact ggagataggc cgaacccagg taggcttccg
     7501 gccccgacga gaaggcccgg ccggtgaacg actcctccgg cgaggtaagg agctttccgt
     7561 actcccccgc cagcgaaagg ttcttgacca ccaggccgaa gtcggtgccg accaggtcgc
     7621 ggaagttgcc gagctggcgg ttgtcgtaac cggccgcgaa ctcggcgtcc cgcgcctcga
     7681 gccgcgaggg ctcgatgatg atcgtgttgg ggtccgggtt gaaccggtgc acgaggctgt
     7741 ccgaatcggc atcggggtac aggttgttgc ggtaggtcat ctccatggcc gtcacgccca
     7801 cgtaggcggc cggcaggaac ttgaagcgga cgttgccgcc cacgatcgtt tcctgcatca
     7861 cgtcgcgcat cggcaggaag tgctcgcgat aatccggctt cagcatgtcc tgg
//
LOCUS       DASVOD010000342         3459 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_963636, whole genome shotgun sequence.
ACCESSION   DASVOD010000342 DASVOD010000000
VERSION     DASVOD010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963636"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VF720_07765"
     CDS             <1..705
                     /locus_tag="VF720_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879290.1"
                     /translation="TATVPGTSTTGRSPGGTVTRRPTSVPRFARFIWLFKVDADTLPD
                     PVVETPYWVTEADAEGNYRLEGLPLDVPFRLMALYDGDRSRGASGANDYWTFDPDTLT
                     LTQAMPVIEGRQVFLVDPRSPGRLSGRMVPPPDSTGADSLSFGVFGARREAGIDSLVM
                     PWPPVRVDRGVRVDRRGEWILSPLEPGRYRLAAWLDTNRNDRFDVNEALGPWIERDVA
                     ADSEISGLELARPSGP"
     gene            720..1604
                     /gene="dapA"
                     /locus_tag="VF720_07770"
     CDS             720..1604
                     /gene="dapA"
                     /locus_tag="VF720_07770"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX7879291.1"
                     /translation="MFEGVLVAVVTPFRNGSVDESAFRAMVDRLFGMGLDGIVVGGST
                     GEAATLSREERRRIVTAAVAETKGRGFVVAGTGTNDTAGSIELSRDAKSAGADGLMIV
                     TPYYNKPSPAGLEAHYRAISEAVPLPILAYNVPGRTGYNLPPALAARLARVPGVVALK
                     EASGSVDQGMDVMMAAPQLTFLSGEDSLTLPLVAAGAKGVVSVAGHLIGDTIKAMIDA
                     HERGEVDEARRLNRTVVAVTRAMFLESNPGPVKYAMSRLGWMANELRPPLAPVTPETA
                     VQIDAVLEPLAGEISRAG"
     gene            complement(1588..1842)
                     /gene="rpsT"
                     /locus_tag="VF720_07775"
     CDS             complement(1588..1842)
                     /gene="rpsT"
                     /locus_tag="VF720_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX7879292.1"
                     /translation="MPHHKSAEKRVLTNERDRQRNVANRSRLKKVMTAQRTLTDSAEA
                     TKGLPGVASEIDKAVKKGLIPRRRADRMKSRAAKAVNRLG"
     gene            2046..>3459
                     /locus_tag="VF720_07780"
     CDS             2046..>3459
                     /locus_tag="VF720_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7879293.1"
                     /translation="MSPSRPRLSERLTADLLEALRRSGPRTGPAAALEAIHAAAGAEW
                     TGLDLTDFAVSGDGSPIPMAAIGRHPESVAVTDGRSITLPGGDVATIRLEFGPKGGPP
                     AGLPAALDAIEPILALSLANGLLASKLSRQDLLVRCADDLLRRVAGGEGEEQLLGSMC
                     AILLDAFPVDRVSVYSITDDHQWVVRRAVSDRRPAPADAEAPPIPYSEIPAERRRPDQ
                     NDLLEYQDLLEADVPEVWRDAARRAGVRSAILAPIVHGGRLWGIVALSRVMTGAVLDT
                     PAAALLSQVLRMTAPILHNERMMRSLSRGLASLEVLLETSREVSSVLDLSEVPRVVAR
                     RAMDLTEADEGVVFLVEPGTNRLKPVLALSPFEAEIMNLEIALGEGVTGQVASMRVGE
                     YVNRVDLDPRSKYIPGTPTVPEALLAAPLVCADRLIGVMTLYKLQGRTFDDVELATIN
                     IFATQAAIAIENARLFQRMEE"
BASE COUNT          515 a         1161 c         1227 g          556 t
ORIGIN      
        1 accgcaacgg tgcccggtac gtcgacgacg gggaggagcc cgggcggcac cgtcacgcgc
       61 cggccgacat cggtgccgcg cttcgcccgt ttcatctggc tcttcaaggt cgatgccgac
      121 acgctccccg acccggtggt cgaaacgccc tactgggtca ccgaggccga tgccgaggga
      181 aactaccgac tcgaggggct gccgctcgat gtgccgttcc ggttgatggc gctgtatgat
      241 ggcgatcgaa gccgcggcgc aagcggggcc aacgactact ggacgttcga tcccgacacg
      301 ctcaccctga cacaggcaat gccggtcatc gagggacgcc aggtcttcct ggtcgacccg
      361 aggagcccgg ggcgtctttc gggacggatg gtgccgccgc ccgactcgac cggcgccgac
      421 agcctgtcgt tcggcgtgtt cggcgcgcgg cgcgaggcgg ggatcgacag cctggtcatg
      481 ccgtggccgc cggtccgggt ggatcggggc gtacgcgtcg atcggagagg ggagtggatc
      541 ctctcgccgc tcgaaccggg gcgctaccgg ttggccgcct ggctggatac gaatcgcaac
      601 gaccgcttcg acgtgaacga ggcgctgggc ccgtggatcg agcgggacgt ggccgccgac
      661 tcggaaatat cgggactgga actggcccgc ccgtcgggcc cctgagaaag gacaaggcaa
      721 tgttcgaggg cgtgttggtg gcggtggtca ccccgttccg gaacgggtcg gtggatgaat
      781 cggcattccg ggccatggtc gaccggctct tcgggatggg gctcgacggt atcgtcgtcg
      841 gaggttcgac gggcgaagcc gccacgctca gccgggagga gcggcgccgg atcgtgacgg
      901 ccgccgtggc cgagacgaag ggacgcggct tcgtggtggc cggcaccggc accaacgaca
      961 cggccggatc gatcgagctc tcgcgcgacg cgaagagtgc gggcgccgat ggcctgatga
     1021 ttgtgacacc ctattacaac aagccgagcc cggccggcct cgaagcgcac tatcgcgcca
     1081 tcagcgaggc ggtgcctctc ccgatccttg cgtacaacgt gccgggacgg accggctaca
     1141 acctgccgcc ggccctggcg gcgcgccttg ccagggtccc cggcgtcgtg gcgctcaagg
     1201 aagcctccgg ctcggtcgac cagggaatgg atgtgatgat ggcggcaccg caactcacgt
     1261 tcctgtcggg cgaggattcg ctcacgctgc cgctcgtggc ggcgggtgcg aagggtgtgg
     1321 tgtcggtggc ggggcacctg atcggcgaca cgatcaaggc gatgatcgac gcgcacgaac
     1381 ggggcgaggt ggacgaggcg cgccgcctca atcgcacggt ggtggccgta acgcgggcca
     1441 tgttcctcga gtccaacccc ggtccggtga agtacgcgat gtcacggctc ggatggatgg
     1501 ccaacgaact gcggccgccc ctggcgcccg tgacgccgga gacggccgtt cagatcgatg
     1561 ccgtgctgga accgctggcc ggggaaatca gccgagccgg ttgaccgcct tggccgcgcg
     1621 cgacttcatg cggtcggcgc gccggcgggg gatgagcccc ttcttcacgg ccttgtcgat
     1681 ttcggaggca acaccgggca gccccttcgt ggcctcggcc gaatcggtga gcgtgcgctg
     1741 ggccgtcatc accttcttga gacgggaacg gttggccacg ttgcgctgcc gatcgcgctc
     1801 gttcgtgaga acgcgcttct cggcggactt gtgatgaggc attgtcggga actccttgcc
     1861 ggttgtcgga aagccgccgc cgtggcgggc tccgtccagg gtcattattc aagcaggcgc
     1921 gagaggttaa agccttgtgg cgctttcgtc aaggggagat tcgcccgctg gccgggctca
     1981 agtcggcctg tgggagtgcc gatgacatgg gtggattcga ccagcacccg tcctcggaga
     2041 ctcgaatgtc gcccagtcgc ccccgccttt ccgagcgcct caccgccgac ctcctggagg
     2101 ccctgcgccg ttccgggccc cgtacgggcc ccgcagcggc cctcgaggcg atccacgcgg
     2161 ccgccggtgc ggagtggacc gggctcgacc tgaccgactt cgccgtgagt ggtgacggat
     2221 cgcccattcc gatggcggcc attggccgcc atccggaaag cgtggcggtt acggacggcc
     2281 ggtcgatcac cctcccgggc ggcgacgtcg ccacgatccg cctcgagttc ggcccgaagg
     2341 ggggcccgcc ggccggcttg ccggccgcgc tcgacgcgat cgagccgatc ctggcgctga
     2401 gccttgccaa cggcctcctg gcctccaagc tcagccggca ggacctcctg gtgcgatgcg
     2461 ccgatgacct cctccgacgg gtggccggcg gcgaagggga ggagcagctc ctcggctcga
     2521 tgtgcgcgat cctgctcgac gcctttccgg ttgatcgcgt gtcggtctac tcgatcaccg
     2581 acgaccacca gtgggtcgtg cggcgagcgg tgtccgaccg gcggccggcg cccgcggatg
     2641 cggaggctcc gccgatcccg tattccgaaa ttccggccga acggcggcgc cccgaccaga
     2701 atgacctgct cgaataccag gacctcctcg aagccgatgt gccggaagtc tggcgcgacg
     2761 cggcccggcg ggccggtgtc cgttccgcca tcctggcgcc gatcgtccat ggcgggcggc
     2821 tctggggaat cgtggccctc tcgcgggtga tgaccggcgc cgtccttgac acgcccgcgg
     2881 cggcgctcct ctcgcaggtg ctccgcatga ctgcgccgat cctccacaac gagcggatga
     2941 tgcgcagtct ttcccgcggc ctggcgagcc tcgaggtcct tctcgagacg agccgcgaag
     3001 tctccagcgt gctcgacctc tccgaagtac cgcgcgtggt ggcccgccgc gcgatggacc
     3061 tgacggaggc cgatgagggg gttgtcttcc tcgtcgagcc gggcactaat cgcctcaagc
     3121 cggtactggc cctttcgccg ttcgaagcgg agatcatgaa cctggagatc gccctgggcg
     3181 aaggggtcac cgggcaagtg gccagcatga gggtcggtga gtacgtcaac cgcgttgacc
     3241 tcgacccccg ttcgaagtac atccccggca cgcccaccgt gccggaggcg ctgctggcgg
     3301 ctccgctggt ctgcgccgat cgcctgatcg gcgtgatgac gctctacaag ctgcaggggc
     3361 gcaccttcga cgacgtggag ctggccacga tcaacatctt cgccacccag gcggccatcg
     3421 ccatcgagaa cgcccgcctc ttccagcgca tggaggagg
//
LOCUS       DASVOD010000343         3039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1017074, whole genome shotgun sequence.
ACCESSION   DASVOD010000343 DASVOD010000000
VERSION     DASVOD010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017074"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VF720_07785"
     CDS             <1..1185
                     /locus_tag="VF720_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7879294.1"
                     /translation="GYLAYSGGAYPDLWLMLGDNAYESGTDTEYQAAVFDVYAPFLRR
                     SPLWPTRGNHDAIYAGGSNDYYDIFTMPTAGQAGGNSSGTEAWYSFDHGDVHLICLDS
                     EGSARTPGSPMLTWLAADLAASTKKWTIAFWHHPPYTKGSHDSDIDSDSGGRMRDMRV
                     NVLPVLEAGGVDLVLCGHSHSYERSYLLDEHYGYSASLVDSMKLDDGDGRPTGDGAYV
                     KPTAGHSAPHEGAVYAVAGSSSRTGGGALNHAAMFRSLDILGSMELTIDDAQLNATFI
                     DDDGLVQDSFVMVKGAVSGVPDGGPVTGPIILTGRPNPFRTLWQLSGTLPSEGPTRVE
                     ILDPSGRLVRTLVDAVRPAGPFDFVWDGRNDGGQAVGAGIFFARVVHGDRAASIKIVR
                     SR"
     gene            1350..1925
                     /locus_tag="VF720_07790"
     CDS             1350..1925
                     /locus_tag="VF720_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879295.1"
                     /translation="MFTRKRTPWLAAVALGAVLALAGGCGDDDDDDGDGGNGDFELRD
                     GTYSVAITTTLSGTGQGCSGTEGPLTLQFPFCGVDDPGDVAGGEDGEMCDVDGDEDDF
                     EFHCTSADTVGGCIYTLHSNGQGSFEDDNFTMTITSFETVSPTTPPCSADPCTTTTTL
                     TGTWLNSSCAGAPAPPLRSMIRTARQLAGGH"
     gene            2019..>3039
                     /locus_tag="VF720_07795"
     CDS             2019..>3039
                     /locus_tag="VF720_07795"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879296.1"
                     /translation="MLLSQGFVLQDRYRIEMLLGRGGMGAVYKAIDTRLGHAVAIKQN
                     LFETSDGTAPISHPTRPDDTEARRTQFEREARLLAALKHAGLAKVTDYFFIPGEGQYL
                     VMDFIEGQDFGAVVSQLGALREEHAAGFIIEIAQVLEYLHGQSPPIIHRDIKPANIRV
                     TPGGQVFLVDFGVAKVGTGEKTMFGAQAVTPGFSPLEQFGGDKPTDERADVYSLAATL
                     YTLTLGKQPPSAIDVLRGRPLFDPNGNTGLRPALLDVINKGMALEPEDRYPNMTAFRE
                     ALHAVFPDRNQKVQNRDTVNLVTQIYTQPMASPGSEMGVYGPTGQPGTAPSRGTMPPS
                     GAPGTM"
BASE COUNT          511 a         1058 c          995 g          475 t
ORIGIN      
        1 ggctacctgg cctattccgg cggcgcgtac cccgatctct ggctgatgct gggcgacaac
       61 gcctacgaat ccggcaccga caccgaatac caggcggcgg tcttcgacgt gtacgcgccg
      121 tttctccgcc ggagcccgct ctggcccacc cgcggcaatc acgacgccat ctatgccggc
      181 ggttcgaacg actactacga cattttcacg atgccgacgg ccgggcaggc cggcggcaac
      241 agctccggca ccgaggcctg gtattcgttc gaccacggcg acgttcacct catctgcctg
      301 gattccgagg gttcggcacg caccccgggg agcccgatgc tcacctggct ggctgccgac
      361 ctcgccgcga gcacgaagaa gtggacgatc gccttctggc atcacccgcc gtacaccaag
      421 gggagccacg attcggacat cgacagcgac tccggcgggc ggatgcggga catgcgcgtg
      481 aacgtcctgc cggtgctcga ggccgggggc gtcgacctcg tgttgtgcgg gcacagccac
      541 agctacgaac ggtcgtatct cctggacgag cattacggct actcggcgag cctggtcgat
      601 tcgatgaaac tggatgacgg cgacggccgg ccgacgggtg acggcgccta cgtgaagccg
      661 acggccggcc attccgcgcc gcatgagggg gccgtgtacg cggtggccgg ttcttcctcg
      721 cgcaccggcg gtggtgcgtt gaaccatgcg gccatgttcc ggtcgctcga tatcctcgga
      781 tcgatggagc tgacgatcga cgatgcgcaa ctcaacgcca cgttcatcga cgacgacggc
      841 ctcgtccagg acagcttcgt gatggtaaaa ggcgccgtct caggcgtgcc ggatggaggg
      901 cccgtgaccg ggcccatcat cctgaccggc cgccccaacc cctttcgcac gctctggcag
      961 ctttcgggca cgcttccgtc ggagggccca acccgcgtcg agatcctcga cccctccggg
     1021 cggctcgtcc gtacccttgt ggatgccgtc cgcccggccg ggcccttcga cttcgtatgg
     1081 gatggccgga acgacggcgg ccaggcggtc ggtgcgggaa ttttcttcgc gcgggtcgtc
     1141 cacggcgatc gcgcggcgtc gatcaagatc gttcgcagcc gctgacgcac cggggaaatc
     1201 cccacccggc cgcccgagcc gagtggtatt ccggggctca atcgccttgt cggagccatt
     1261 ccatctgtga gatacacaat gccggcggcg acggcgggag aggcccgcca cggtccccct
     1321 cttggaaggc tcacgacaag gagtcgagca tgttcacacg gaaacggacg ccctggctgg
     1381 cggcggtggc cctcggcgca gtcctcgcgc tggccggcgg gtgcggtgat gacgatgatg
     1441 atgatggcga cggcggcaac ggcgacttcg agcttcgcga cggcacctac agtgtggcga
     1501 tcacgaccac gttgagcggt acaggccagg gatgttcggg caccgaaggt ccgctcacgc
     1561 tccagttccc gttctgcggg gtcgatgacc ccggtgatgt cgccggtggc gaggatggcg
     1621 agatgtgcga cgtcgacggg gacgaggatg acttcgagtt ccactgcacc tcggccgaca
     1681 cggtcggagg gtgcatctac accctgcaca gcaacggcca gggctcgttc gaggacgaca
     1741 acttcacgat gacgatcacg tcgttcgaga cggtcagccc gaccacgccg ccctgttcgg
     1801 ccgatccgtg caccaccacc accaccctca ccggcacctg gctcaattcg agctgcgccg
     1861 gggcgcccgc gccgccgctt cgttcgatga tccggacggc ccgacagctg gccggaggtc
     1921 actgacccac cccccggaca ggggcatccg gcctccctcg ttgaaccgga gggcccggta
     1981 acggataatc cggcgtcctc caaccagacg gagaccggat gcttctgtcc cagggttttg
     2041 tcctgcagga ccgctaccgc atcgagatgc ttctcggacg cggtggcatg ggcgcggtgt
     2101 acaaggccat cgatacgcgc ctcggccacg cggtggccat caagcagaac ctgttcgaga
     2161 cgtcggatgg cacggcgccg atcagtcatc ctacccgccc cgacgacacc gaggcccgcc
     2221 gcacccagtt cgagcgcgag gcgcgcctcc tggccgccct gaagcacgcg ggcctggcca
     2281 aggtcaccga ctacttcttc atccccggcg aaggccagta cctggtcatg gacttcatcg
     2341 aggggcagga cttcggcgcg gtggtgagcc agctgggcgc actgcgcgag gagcacgccg
     2401 ccggcttcat catcgagatc gcccaggtgc tggaatacct gcacgggcag tcgcctccca
     2461 tcatccaccg cgacatcaag ccggccaaca tccgggtgac gcccgggggg caggtcttcc
     2521 tggtcgattt cggcgtggcc aaggtgggca cgggcgaaaa gacgatgttc ggcgcccagg
     2581 cggtgacccc gggcttcagc ccgctggagc agttcggagg ggacaagccc accgatgaac
     2641 gggcggacgt ctactccctt gcggccacgc tctacacgct gaccctgggg aagcagccgc
     2701 catcggctat cgacgtcctc cgcggccggc ccctcttcga tccgaacggg aacaccggtc
     2761 tccggccggc gctgctcgac gtgatcaaca aggggatggc cctcgaaccc gaagaccgct
     2821 acccgaacat gacggccttc cgcgaggcac tccacgccgt cttcccggat cgcaaccaga
     2881 aggtgcagaa ccgggacacc gtgaacctcg tcacgcagat ctacacgcag ccgatggcgt
     2941 cgcccggctc ggagatgggc gtgtacggcc cgaccggcca gcccggcacg gcgccctcgc
     3001 ggggcacgat gcccccttcg ggcgcgccgg gcacgatgc
//
LOCUS       DASVOD010000344         2920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_76597, whole genome shotgun sequence.
ACCESSION   DASVOD010000344 DASVOD010000000
VERSION     DASVOD010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76597"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VF720_07800"
     CDS             complement(<1..720)
                     /locus_tag="VF720_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879297.1"
                     /translation="MTRIAALGVAALIPAALLLSQPSRATSLADGYLDPDRVASELRS
                     VAGKLGGARVVEYGRTRSGKPLLALEVHRGEGDPTLHPALLVVGGCEPERLATVEMAL
                     AIAARAGAAPDTGADPLATNVLYVIPDAAPDGRAHVLTGGGGWIGGSIDDDGDGRADE
                     DGPDDLDGDGSLRWMRVWRAGGAWRADTADVRASVKADPAGGKPGQFDLVREGKDDDK
                     DERLNEDGAGGVRFDANFPHAW"
     gene            905..1567
                     /locus_tag="VF720_07805"
     CDS             905..1567
                     /locus_tag="VF720_07805"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879298.1"
                     /translation="MIARHPSRRLLAPVLMLAGILVTSPSTLVQASGSSGGGSSSSDS
                     PAIPESQQMSPEEQAAAARQAAMETYDKNYELTLKAADAMDEARQRKAQGPEEAKKAE
                     ELRAQALKHYKKAIDGFRKAVKLDPGYHEAWNMLGYSYRKTGQMGEAFKAYETCLKLK
                     PDYDLAHEYLGEAWLQAGDIDKARAELAWLTEQKSPEAPKLAAAIGKVAAGETAFLGT
                     DW"
     gene            1592..2020
                     /locus_tag="VF720_07810"
     CDS             1592..2020
                     /locus_tag="VF720_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7879299.1"
                     /translation="MAGGKDSDLGDWGRLFLRVIVGMGIMTHGYPKVFGVNEEGVRRI
                     VGFTRGVADMGFPYPEYFAWAAALSEFVGGAMLVLGLGTRVAAFMIAGTMIVALYKSR
                     AKGWDGMELASLYLASAVFLLLNGPGRKSLDALLFKRKGG"
     gene            2091..2426
                     /locus_tag="VF720_07815"
     CDS             2091..2426
                     /locus_tag="VF720_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis-associated
                     peroxidase-like protein"
                     /protein_id="HEX7879300.1"
                     /translation="MHTYYNPADLARFGEIAEGSPELWEKFLAWYGAVFSEGALTARE
                     KSLIALGVAHAVQCPYCIDAYTKDCLEKGSNLDQMTEAVHVAGAIRGGASLVHGVQMK
                     NVVQKVEMP"
     gene            2423..>2920
                     /locus_tag="VF720_07820"
     CDS             2423..>2920
                     /locus_tag="VF720_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7879301.1"
                     /translation="MTIGTGSLLRRGSPLASAAEQVRRLESTTAIPPFTTRIKDAGLP
                     ELTATGIQVMQINVGKQCNQSCAHCHVDAGPDRLEQMTRETVDLCLSVLAWTDIPTVD
                     ITGGAPELNPEFRRLVVDARKLGRRVMDRCNLTVLGQRGQEDLAAFLAEQRVEIVASL
                     PYYQER"
BASE COUNT          489 a         1008 c          981 g          442 t
ORIGIN      
        1 ccaggcgtgg gggaagttgg cgtcgaagcg cacgccgccg gcgccgtctt cgttgaggcg
       61 ctcgtccttg tcgtcgtcct taccttcgcg caccaggtcg aactgcccgg gcttgccgcc
      121 cgccggatcg gccttcacgg aggcgcggac gtcggccgtg tcggcccgcc aggcgccgcc
      181 ggcgcgccac acgcgcatcc agcggaggga gccgtcaccg tccaggtcgt cgggtccgtc
      241 ctcgtcggcc ctgccgtcgc cgtcgtcgtc gatcgatccg ccgatccagc cgccgccgcc
      301 ggtcagcacg tgcgcgcgcc cgtcgggcgc cgcatcggga atgacgtaca ggacgttcgt
      361 ggccagcgga tcggcgccgg tatccggggc ggctcctgcc cgtgcggcga tggccagcgc
      421 catctccacc gtggccagcc gttccggctc gcatcccccc accacgagga gcgcggggtg
      481 gagcgtcggg tctccctcgc cgcggtgcac ttccagggcc aggagcggct ttccggagcg
      541 cgtgcgtccg tactccacca ccctggcgcc gccgagcttg ccggccacgc ttcgcaactc
      601 ggaggccaca cggtccggat cgaggtaccc gtccgccagt gacgtggcgc gcgagggttg
      661 ggacaaaagg agggccgcgg ggatcaacgc agccacgccg agtgcggcga ttcgggtcaa
      721 ggggacctcc ggaaacatga gcgggcgccg ggccgggccg cccagggggg gatggccggg
      781 gtggggcggg cgcatctcat cacacccccg ggcgggcatc aagccgaatc cgccttgtcc
      841 cgccccgggc cccaccctat cctttccctc ttccgatcgg actcaccaat cagcgaggcc
      901 tcacatgatt gcgcgccatc cctcccgccg actcctggcc ccggtgctca tgctggccgg
      961 cattctggtc acatccccct cgaccctggt ccaggcctcc ggatcgagcg ggggaggctc
     1021 gtcatcctcc gattcgccgg ccatcccgga atcgcagcag atgtctcccg aggagcaggc
     1081 ggcggccgcg cgccaggccg ccatggagac ctacgacaag aactacgagc tgaccctgaa
     1141 agccgccgac gccatggacg aggctcgcca gaggaaggcc cagggaccgg aggaagcgaa
     1201 gaaggcggag gagcttcggg cgcaggccct gaagcactac aagaaggcca tcgacggctt
     1261 caggaaggcg gtcaagctcg atcccggtta tcacgaagcg tggaacatgc tcggctacag
     1321 ctaccgcaag acaggccaga tgggcgaggc cttcaaggcg tacgaaacct gcctgaagct
     1381 caagcccgat tacgatctcg cgcacgagta cctgggcgag gcctggctgc aggccggcga
     1441 catcgacaag gccagggccg agctggcctg gctcaccgag cagaagtctc cggaagcgcc
     1501 gaagctggcg gcggccatcg ggaaggtggc ggcgggagag accgccttcc tgggaaccga
     1561 ctggtaatca gccaacgagg gggaggggac gatggcggga ggcaaggatt cggacctggg
     1621 agattggggg cgtctctttc tccgcgtgat tgtcggcatg gggatcatga cgcacggcta
     1681 tcccaaggtg ttcggggtca atgaggaagg agtgcggcgg atcgtcggct tcaccagggg
     1741 cgtggcggac atgggatttc cgtaccctga atatttcgcc tgggctgccg cgctctccga
     1801 gttcgtcggc ggcgccatgc tcgtcctcgg cctcggcacg cgcgtggccg ccttcatgat
     1861 cgcgggcacg atgatcgtgg cgctctacaa gagccgcgcg aaggggtggg acggcatgga
     1921 gctggcctcc ctctacctgg cgtcggccgt cttccttctc ttgaacggcc ccggccggaa
     1981 atcgctcgac gcgctgctgt tcaagcgcaa gggtggctga tcgggcgaac cgaactgcta
     2041 tccgcaactc tccaactcga gtccgcccga tcatcccgac aggagacatg atgcacacct
     2101 actacaaccc ggccgacctg gcccgctttg gcgagatcgc cgagggaagc ccggagctct
     2161 gggagaaatt cctcgcctgg tacggcgccg tcttctccga aggcgcgttg accgcccgcg
     2221 aaaagtcgct catcgcactc ggcgtggcgc atgccgtgca atgtccgtat tgcatcgacg
     2281 cctacacgaa ggactgcctc gaaaagggaa gcaacctcga tcagatgacc gaggcggtcc
     2341 acgtggccgg cgccatccgc ggcggtgcca gcctcgtgca tggcgtgcag atgaagaacg
     2401 tggtccagaa agtcgagatg ccgtgacgat cggcaccggg agcctccttc gccgcggctc
     2461 gccgctggca tcggcggcgg agcaggtccg tcggctcgaa tcgacgaccg ccatcccacc
     2521 cttcacgacc cgcatcaagg atgccggcct ccccgaactc accgccaccg gcatccaggt
     2581 gatgcagatc aacgtcggca agcagtgcaa ccagagctgc gcgcactgcc acgtggatgc
     2641 cggacccgac cgtctcgaac agatgacgcg cgagacggtc gatctctgcc tctcggtgct
     2701 ggcctggacc gacatcccca cggtcgatat caccggcggc gcgccggagc tcaatcccga
     2761 attccggcgc ctcgtcgtcg atgcgcgcaa gctggggcgt cgcgtgatgg accgctgcaa
     2821 cctcaccgtg ctcggccagc gcggccagga ggatctcgcg gccttcctcg ccgaacagcg
     2881 cgtcgagatc gtcgcctcgc ttccgtacta tcaggagcgc
//
LOCUS       DASVOD010000345         3854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_128848, whole genome shotgun sequence.
ACCESSION   DASVOD010000345 DASVOD010000000
VERSION     DASVOD010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128848"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VF720_07825"
     CDS             complement(<1..235)
                     /locus_tag="VF720_07825"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX7879302.1"
                     /translation="MSGSSLARRVASGLVLAPLVFYLVYLGSWPFLLFIEAMVILGSI
                     EFLRMARNKGAEPHMELGVLAAAALAALLYEGHA"
     gene            complement(232..984)
                     /locus_tag="VF720_07830"
     CDS             complement(232..984)
                     /locus_tag="VF720_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX7879303.1"
                     /translation="MPTTTELDALREELKSAGPIPRHVAVIMDGNGRWAKERGQPRIF
                     GHQAGRKPVREVVEGCRELGIGTLTLYTFSVENWQRPAAEIKALMAFLEATLREEKAE
                     LKKNGIRLGVVGRPQDLPAPVQKALNDTIEELSGGRGMLLNLALSYGGRTEIVDAARA
                     IAEAAQDGSFEPSKLDEAMFARHLYTADIPDPDLLIRTSGELRLSNFLLWQLAYAEIW
                     VTDLYWPDFRKEHLFEAVRDFQRRDRRFGRVT"
     gene            complement(1043..1603)
                     /gene="frr"
                     /locus_tag="VF720_07835"
     CDS             complement(1043..1603)
                     /gene="frr"
                     /locus_tag="VF720_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005386731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX7879304.1"
                     /translation="MSAKEVLSTAEEKMKKSVETVRHEFTGIRTGKATAALLDTVKVD
                     YYGSMMPVNQVASISVPEPRLLVVQPWEKAMLQPIARAIQASDLGLNPQDDGSVLKIP
                     VPTLNEDRRKDLVKLVKKFAEEGRVAIRNVRHHANDELKKALKDKTISEDDEKRAMAD
                     CQKITDKYVGQIDELIAKKEAEVMEV"
     gene            complement(1609..2328)
                     /gene="pyrH"
                     /locus_tag="VF720_07840"
     CDS             complement(1609..2328)
                     /gene="pyrH"
                     /locus_tag="VF720_07840"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX7879305.1"
                     /translation="MAERRYRRILLKLSGEVLAGGESHGLHAGRLGEIADELKRVHET
                     GVEMGVVVGGGNIYRGLSGAADGMDRVVGDQMGMLATVINSLALQDALEQRGVKTRVM
                     SAIDMQRIAEPFIRRRAIRHLEKGRVVILAAGTGNPYFTTDTAAVLRAIEIQADVVIK
                     ATKVDGVYTDDPMKVPTATLYDRVTYKDVLLRELKVMDSTAFQLCRDNNLPIVVFNVN
                     VSGNLLRLIQGETIGTLVMEG"
     gene            complement(2340..2939)
                     /gene="tsf"
                     /locus_tag="VF720_07845"
     CDS             complement(2340..2939)
                     /gene="tsf"
                     /locus_tag="VF720_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX7879306.1"
                     /translation="MTITANQVSELRARTGAGMMDCKKALSETGGDIDAAIEHLRKTG
                     IAKAEKKSDREASEGLVHSYIHPGGRVGVLIQVNCETDFVAKTEDFQSLVRDLAMQVA
                     ATSPRGVRREDIDETIVAKEREVFAAQMADQKKPAEVMERIISGKIDKFYAETVLLEM
                     PFIKDDSKTVDTLVKEISGKLGENIVVRRFARFALGEGS"
     gene            complement(3081..3848)
                     /gene="rpsB"
                     /locus_tag="VF720_07850"
     CDS             complement(3081..3848)
                     /gene="rpsB"
                     /locus_tag="VF720_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004891029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX7879307.1"
                     /translation="MLEAGVHFGHRTRRWNPKMKKYIFIERNGIYIVDLQKTMTCIEQ
                     ATNALKRLVADGGTVLFVGTKKQAQEPIREEAGRSGMYYVTERWLGGMLTNFKTVKQS
                     VARLHELDRMATDGTFEVLTKREASTLDKERGKLEKVFSGIKEMRDLPAALFVVDTKK
                     ERIAIMEANRLGIPIIGIVDTNCDPDVIDYPIPGNDDAIRSIRLFARMVSDLVLESRR
                     EKPAIKDVDFSEGYSAGGDDDDLVSSPGEAAPTVAPA"
BASE COUNT          586 a         1244 c         1279 g          745 t
ORIGIN      
        1 ccgcgtgccc ttcatacagg agcgccgcca gcgcggccgc cgccagaact ccgagctcca
       61 tgtgcggctc ggctcccttg ttgcgggcca tgcggaggaa ttcgatcgaa cccaggatga
      121 ccatggcttc gatgaagagg aggaagggcc agctgccgag gtaaacgaga tagaaaacca
      181 gtggcgcgag gaccagtccc gacgccaccc gtcgcgcgag cgacgagccg ctcacgtcac
      241 ccggccgaat cggcggtcgc gccgctggaa atcgcggacg gcctcgaaga gatgctcctt
      301 ccggaagtcg ggccagtaga ggtcggtcac ccagatctcg gcgtacgcga gctgccacaa
      361 caggaaattc gagaggcgca gctcgccgga ggtgcggatc aacaggtcgg gatcgggaat
      421 gtcggcggtg tagagatgac gcgcgaacat ggcctcgtcg agcttcgacg gttcgaacga
      481 gccatcctgc gcggcctcgg cgatggcacg cgccgcatcc acgatctcgg tgcgcccgcc
      541 gtacgagagc gccaggttga gcagcattcc ccgcccgccc gaaagctcct cgatggtgtc
      601 gttgagcgcc ttctgcaccg gcgcgggcaa atcctgtggc cgccccacca ccccgaggcg
      661 gatgccgttc ttcttgagct cggccttctc ctcgcggagc gtggcctcga ggaaggccat
      721 gagcgccttg atctcggccg cggggcgctg ccagttttcc acgctgaagg tgtagagcgt
      781 cagcgtgccg atgccgagct cgcggcaccc ttccaccact tcgcggaccg gcttgcggcc
      841 ggcctggtgg ccgaagatgc ggggctggcc gcgctccttg gcccatcggc cgttgccgtc
      901 catgatgacg gccacgtgac gcgggatggg gccggcggac ttgagctcct cgcggagggc
      961 gtcgagttcg gtggtggtgg gcatcggcct cggtggttgg agatccaaga acctgtcaaa
     1021 ccttcgctgc gcgaaggctc gactagacct ccatcacctc cgcctctttc ttcgcgatca
     1081 actcgtcgat ctggccgacg tacttgtcgg tgatcttctg gcagtcggcc atggcgcgct
     1141 tctcgtcgtc ctcggagatg gtcttgtcct tcaacgcctt cttgagctcg tcgttggcgt
     1201 ggtggcgcac gttgcggatg gccacgcgcc cctcctcggc gaacttcttg accagcttta
     1261 cgaggtcctt gcggcgatcc tcgttgagcg tgggcaccgg gatcttgagg accgagccgt
     1321 cgtcctgcgg gttgagcccg aggtcggagg cctggatggc ccgcgcgatc ggctggagca
     1381 tggccttctc ccagggctgg acgaccagca gccggggttc gggcaccgag atactggcca
     1441 cctggttcac cggcatcatc gatccgtagt agtcgacctt gacggtgtcg aggagcgcgg
     1501 cggtggcctt gccggttctg attccggtga actcgtggcg cacggtctcc accgacttct
     1561 tcatcttctc ttcggcggtc gaaaggactt ccttggcgga catcggtgct agccctccat
     1621 gaccagggtg ccgatggtct ctccctggat gagccggagc agattgccgg aaacgttcac
     1681 gttgaatacc acgatcggca ggttgttgtc gcggcagagt tggaacgccg tggaatccat
     1741 caccttgagc tcccggagga gcacatcctt gtacgtgacc cgatcgtaca acgtggcggt
     1801 gggcactttc atcgggtcgt cggtataaac cccgtcgacc ttggtggcct tgatgaccac
     1861 gtcggcctgg atctcgatgg cccggagcac cgccgccgtg tcggtcgtga agtagggatt
     1921 gccggtcccg gccgccagga tgaccacccg ccccttctcg aggtggcgga tggcccggcg
     1981 gcggatgaac ggctcggcga tccgctgcat gtcgatggcc gacatcacac gcgtcttgac
     2041 cccccgctgc tccagggcat cctggagcgc cagcgagttg atgacggtgg ccagcatccc
     2101 catctggtcg cccaccaccc ggtccatgcc atcggcggcg ccggagaggc cgcgatagat
     2161 gttgccgccc cccaccacca cgcccatctc gacgccggtt tcgtggacgc gcttcagttc
     2221 gtcggcgatt tcgcccagcc gtccggcgtg caggccgtgg ctttcgccac cggccagcac
     2281 ttcaccggag agcttgagga ggatccgtcg gtaccggcgc tcggccatac cggggtccgt
     2341 tacgagcctt cgcccagcgc gaaccgggcg aagcggcgga cgacgatgtt ctcgccgagc
     2401 ttgcccgaga tttccttgac cagcgtgtcg accgtcttcg agtcgtcctt gatgaacggc
     2461 atctcgagca ggacggtctc ggcgtagaac ttgtcgatct tgccggagat gatccgctcc
     2521 atcacctcgg ccggcttctt ctggtcggcc atctgggcgg cgaacacctc gcgctccttg
     2581 gccacgatgg tctcgtcgat gtcctcccga cgcacgcccc tggggctggt ggcggccacc
     2641 tgcatggcca ggtcgcggac gaggctctgg aagtcctcgg tcttggccac gaagtcggtc
     2701 tcgcaattca cctggatgag cacgcccacg cggccacccg gatggatgta cgagtgcacg
     2761 agcccctcac tggcctcgcg atcggatttc ttctcggcct tggcgatgcc ggtcttgcga
     2821 aggtgctcga tggccgcgtc gatgtcgccg ccggtctccg acagcgcctt cttgcaatcc
     2881 atcatgcccg cgccggtccg ggcacgcagc tcggacacct ggttggcggt gattgtcatg
     2941 gcaggtctcg ctttctatgt cgggtggaga tagtcgattt tcgagttggc tgatgagtcc
     3001 gtgcgtaccg ggcgccggcc acgacgggcg ttgcccgtcg cgtgtcgccg ggcggaccct
     3061 gccgggagac ggcctcgtgc ttaagcggga gcgacggtcg gggcagcctc gcccggcgac
     3121 gatacgaggt catcatcatc cccgccggcc gagtaccctt cggagaagtc gacatccttg
     3181 atggccggct tctcgcggcg cgactcgagc accaggtcgg aaaccatgcg ggcgaagagc
     3241 cggatcgagc ggatggcgtc gtcgttcccc gggatcgggt agtcgatgac gtccggatcg
     3301 cagttggtgt cgacgatgcc gatgatcggg atgccgaggc ggttggcctc catgatggcg
     3361 atgcgctcct tcttcgtgtc gaccacgaag agggccgccg ggaggtcacg catctccttg
     3421 atgccggaga agaccttctc gagcttgccg cgctccttgt cgagcgtgga ggcctcgcgc
     3481 ttggtgagca cctcgaaggt gccgtcggtg gccatgcggt cgagctcgtg gagacgggcc
     3541 acgctctgct tgaccgtctt gaagttggtg agcatgccgc ccagccagcg ctcggtgacg
     3601 tagtacatgc ccgaacgccc ggcttcctcg cggatgggct cctgggcctg cttcttggtt
     3661 ccgacgaaga ggaccgtgcc gccgtcggcc acgaggcgct tcagcgcgtt ggtggcctgc
     3721 tcgatgcagg tcatggtctt ctggagatcg acgatgtaga tgccgttgcg ctcgatgaag
     3781 atgtacttct tcatcttggg gttccagcgc cgggtccggt gaccgaagtg gacaccggcc
     3841 tccagcaaat cctt
//
LOCUS       DASVOD010000346         4918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_649152, whole genome shotgun sequence.
ACCESSION   DASVOD010000346 DASVOD010000000
VERSION     DASVOD010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4918
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649152"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF720_07855"
     CDS             complement(<1..334)
                     /locus_tag="VF720_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879308.1"
                     /translation="MMAVRPEFHPVTLPRATTAGLLLAALVGLAGCGKGGHDGAIQAT
                     GHVEAIEVRVSARVGGRLLHFDPIEGSRLAAGDVLAVIDSVDLVADRNRRVADLATAR
                     ANLDLLAAG"
     gene            complement(331..1038)
                     /locus_tag="VF720_07860"
     CDS             complement(331..1038)
                     /locus_tag="VF720_07860"
                     /inference="COORDINATES: protein motif:HMM:NF037007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7879309.1"
                     /translation="MPRPRDAARSRQALLEAGRRLFADHGYAGASVDAICREAGVNKA
                     MVSYHFGGKAGLHGAILADLLDPVVARLTELAASPVDPEAKLRGYFRLMLELHRDRRR
                     FPQMVLREVLAGGDHLDRSVRQRIAGIFDAVRRIIQEGIDAGVFRPVDPFLTYLTLTG
                     SLIFFFATETFRERMLEENRALAAAGGIDPRAVHLPREVPSPEAFVAQAEAIFLSALR
                     RPAGRANDESEPRRFPS"
     gene            1319..1945
                     /locus_tag="VF720_07865"
     CDS             1319..1945
                     /locus_tag="VF720_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7879310.1"
                     /translation="MTSRPDESPERWQELLSRLQPFHESAALTARRLARTADDGDDLL
                     SEAILRAFEKLPSLRDPTRFRSWFFAVLLSVHRNRARRHFWKRFLPLDASNGPEPVGI
                     DGARQAELARRADRARRALNTLNAEQREAIVLFELQGFTIEEIAEMSNVSLSAIKSRL
                     ARGRERMRQQYERWESHVESSRNERADVRRHRESEPGSRSLRDRLVAD"
     gene            2043..2825
                     /locus_tag="VF720_07870"
     CDS             2043..2825
                     /locus_tag="VF720_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879311.1"
                     /translation="MTQTRRLFSDRPWLAPLVAAAAVVAMLVVPFSYQKTTAYDVVFH
                     LDGAAPAVTASLSSALQQSLSPESVNSNSPGDGTVTLTARVPVEKAAGLAVHAASFAN
                     VLAARGLSVTHEVVPVRETVSGNLYAMTTSGAVRVDITTTGRTPEEMEADVRRQLEAA
                     GIANAEVTINKDGTQTTIQVMAHQDENSGEAHEPRDFNFTFDGQAPDPSKTAQIRIDN
                     SGHTMSDAQIKETIESQLASQGHPATVVVSGGKIVSVTPIRN"
     gene            complement(2883..3953)
                     /gene="ispH"
                     /locus_tag="VF720_07875"
                     /gene_synonym="lytB"
     CDS             complement(2883..3953)
                     /gene="ispH"
                     /locus_tag="VF720_07875"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX7879312.1"
                     /translation="MTESDPVRPSSPAATTPKLDIFLARPRGYCAGVDRAIDIVDLAL
                     ELYGPPVYVRHEIVHNSHVVGALRERGAVFVEELSGVPEEAVLVLSAHGVSPTVREEA
                     AARRLKMIDATCPLVTKVHLEALRMEKDGYTIVLIGHRGHTEVEGTMGHVPRSIVLVQ
                     NAAEVADLVVPDPERVAYLTQTTLSLDECAETVAALKARFPKLRSPSSDSICYATQNR
                     QNAVKAVVPRIDLLLVVGSKQSSNSNRLREAGEIRGVRSFLIDNAEEIQAEWLVDARR
                     IGVTAGASTPEYLVEEVLSRLRSEGGKVIEFEVIPEAVNFNLPKNLLDDLKEDPRGEM
                     LLLSAGRSLAGRARGPVDKSGQ"
     gene            4164..>4918
                     /locus_tag="VF720_07880"
     CDS             4164..>4918
                     /locus_tag="VF720_07880"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879313.1"
                     /translation="MSFLTRIFGARTNEHYTRGIVHFNRQEYDNAVGEFELAVAEIQD
                     HADPDYELARFYSAEAHAKLGLARLRTGDRALAEAEFRKALEVSDQFPDLHYHLGVLA
                     EQDGRPDEAEQRLRHALAIHPDYVEARGYLGLTLLGRGRADEAREEIAEAARRGLPLP
                     DGFALIPAADVPAALVDELRTQIEKQSIGSMVLSNALESYSVGQVEAAITELDAAVKE
                     HPTYADLRCRLGRLLAETGKHEEALAQFAAALE"
BASE COUNT          822 a         1713 c         1620 g          763 t
ORIGIN      
        1 tgccggcggc caggaggtcg aggttggccc gcgcggtggc caggtcggcc acgcgccgat
       61 tgcgatcggc caccaggtcg acgctgtcga tcaccgccag cacgtcgccc gcggcgagcc
      121 ggcttccctc gatcgggtcg aagtggagga ggcgtccccc gacacgggcc gagacgcgca
      181 cctcgatggc ctcaacgtgg ccggtggcct ggatggcgcc gtcgtgccct cccttgccgc
      241 agccggccag gccgaccaac gccgcgagca gcagccctgc ggtcgtcgcg cgtggcaggg
      301 tgactggatg gaattcaggt cgaacggcca tcatgacggg aacctccgcg gttcagattc
      361 gtcgttcgcg cggcctgccg ggcggcgcaa cgccgagagg aagatcgctt ccgcctgcgc
      421 cacgaacgct tcgggcgagg ggacttccct ggggagatgc acggcgcgcg ggtcgatccc
      481 gcctgccgcg gccagggccc ggttttcctc gagcatccgc tcgcggaacg tctcggtggc
      541 gaagaagaag atcaggctgc cggtgagcgt gagataggtg aggaacgggt cgaccgggcg
      601 gaacacaccg gcgtcgatcc cttcctgaat gatgcgtcgc accgcgtcga agatcccggc
      661 gatccgctgg cgcaccgacc ggtcgagatg atcgccgccg gccagcacct cgcgcagcac
      721 catctgtgga aaccggcgcc gatcgcgatg gagctccagc atcaggcgga agtagccgcg
      781 gagcttggcc tcgggatcga ccggcgaggc ggccagttcg gtgaggcggg ccactaccgg
      841 gtcgagcagg tcggccagga tggcgccgtg aaggccggcc ttcccgccga agtggtagct
      901 caccatcgcc ttgttcacgc ccgcctcccg gcagatggca tccaccgagg cgccggcgta
      961 gccatgatcg gcaaagaggc gccggccggc ttccaggagg gcctgacggc tgcgggcagc
     1021 atcacggggg cgcggcattt cggatcggcc agactcttga ttcatcagta acctgctctg
     1081 taatagtgaa ttagggttgc actgaccgtg ctgggctcgc tcaacccctc gtttgaatta
     1141 aacgtttagt taataaacga cacccaggcc cccccggtca agcactattt tcaacacaat
     1201 ttcttccgcc cccggcaccc catctgcgac ttttcccgta cccagtttgt ctccgccccg
     1261 ccggacggga cgaccgcccg gcgtgcgcac gcaacacgag tcacgacagg atccgatgat
     1321 gacgtcccga cccgacgaga gtccggagcg ctggcaggaa ctcctgtccc gcctccagcc
     1381 gttccacgaa tcggccgcgc tgaccgcgcg ccgcctcgcg cggacggcgg acgacgggga
     1441 cgaccttctt tccgaggcca tcctccgcgc gttcgagaag ctcccgtcgc ttcgggatcc
     1501 gacccggttc cgatcctggt ttttcgcggt gctcctttcg gtgcatcgca accgtgcccg
     1561 gcgtcacttc tggaagcgct tcctgccgct ggatgcgtcg aacggtcccg aaccggtcgg
     1621 gatcgacggc gcccgccagg cggaactggc gcgacgggcc gatcgggccc gccgggcgct
     1681 caacaccctg aatgccgaac agcgcgaagc catcgtcctg ttcgaactac agggtttcac
     1741 gatcgaggaa atcgccgaga tgtcgaacgt cagcctctcg gccatcaagt cgcgcctcgc
     1801 tcgcggtcgc gagcggatga ggcagcaata tgaaaggtgg gagtcgcatg tcgaatcatc
     1861 gcgaaacgaa cgagcggatg tccggcggca ccgcgaatcg gaacccggga gccggagcct
     1921 ccgtgaccga ctcgtggccg actgactcgg cctcgctcgc cgaggcgacg tcgcgcggac
     1981 tcccgtcatt gactgaatcc gcccggcgcg ccgagcgccg tgcgcatcac ccggagggat
     2041 acgtgaccca gacccgacgc ctgttttcgg accgaccctg gctggcgccg ctcgtcgccg
     2101 cggccgccgt ggtggccatg ctggtcgtgc cgttctcgta ccagaagacc accgcgtacg
     2161 atgtcgtctt ccacctcgac ggcgccgccc cggccgtcac cgccagcctc tcgtcggcgc
     2221 tccagcagtc gctgtcgccc gagtcggtca actcgaactc gcccggcgac ggcaccgtga
     2281 ccctcaccgc ccgcgtcccg gtggagaaag ccgccgggct tgccgtccat gcggcctcgt
     2341 tcgcaaacgt cctcgcggcg cggggcctct cggtcacgca cgaagtcgtt cccgtcaggg
     2401 aaaccgtgtc cggaaacctc tacgcgatga cgaccagcgg cgcggtccgg gtggacatca
     2461 ccaccaccgg tcgcacgccg gaggagatgg aggccgacgt tcgccgccag cttgaggccg
     2521 ccggcatcgc caacgcggag gtcaccatca acaaggacgg gacccagacc accatccagg
     2581 tcatggccca ccaggacgag aactcgggcg aggcgcacga accgcgcgac ttcaacttca
     2641 ccttcgacgg ccaggcgccc gacccgtcga agaccgccca gattcgcatc gacaattcgg
     2701 gtcacacgat gagcgatgca cagatcaagg agacgatcga atcgcaactc gcctcgcagg
     2761 gacatccggc cacggtggtg gtttcgggcg ggaagatcgt ctcggtgacg ccgatcagga
     2821 actgatcagc actcaggttg gaccggaggc gcgtcggccg aaggccgggg cgcctccttc
     2881 cgttattgcc ccgacttgtc cacgggtccc cgggcccgtc ccgccagcga gcgtccggcc
     2941 gacaggagca gcatctcccc gcgcggatcc tccttgagat cgtcgagcag gttcttcggc
     3001 aggttgaagt tcaccgcctc ggggatcacc tcgaattcga tcaccttccc gccttcggag
     3061 cggagccgag acaggacctc ctcgaccagg tattcgggcg tggaggcccc ggccgtgacg
     3121 ccgatgcgcc gggcatcgac cagccactcg gcctggatct cctcggcgtt gtcgatcagg
     3181 aacgagcgca ctccccggat ttcgccggcc tcgcgcaggc ggttggaatt gctgctctgt
     3241 ttcgagccga ccacgagcag gaggtcgatc ctcggcacca cggcctttac agcattctgc
     3301 cggttctgcg tggcatagca gatgctgtcg gacgaagggg aacggagctt cgggaagcgg
     3361 gccttcagcg cggccacggt ttcggcgcac tcatccagcg acagggtcgt ctgggtgaga
     3421 taggccaccc gctcgggatc gggcaccacg agatcggcca cctcggccgc gttctgcacc
     3481 aggacgatcg agcgcggcac gtgccccatc gttccttcca cctcggtgtg cccgcgatgt
     3541 ccgatcagga cgatggtgta gccgtccttc tccatgcgga gcgcttcgag atgtaccttg
     3601 gtgaccaggg gacaggtggc gtcgatcatc ttcaatcgtc gtgcggccgc ctcttcccgc
     3661 acggtggggg agacgccgtg agccgacagg acgaggaccg cctcctcggg gacgcccgac
     3721 agttcctcca cgaagaccgc tccgcgctcg cgcagggcgc caaccacgtg gctgttgtgg
     3781 acgatctcgt gtcgcacata gaccggcggc ccgtacagct cgagagccag gtcgacgatg
     3841 tcgatcgcgc ggtccacccc ggcgcagtaa ccgcgagggc gggccaggaa gatatcgagc
     3901 ttcggagtgg tggcggcggg ggatgagggg cgaaccggat cgctttcagt catgaccctg
     3961 aaatgatatc aggccggcga tgggatcgca cgccggcagg cagccgcaat gttccattgt
     4021 cgggcgcacg ctccgccggc agccggcgtc tgtccgcggc aactggcatc ccaccccacg
     4081 gtctgctacg ttggatcgag cgggttccgg tgcaggtcgc gcggcgcgcg cgacgcaccc
     4141 agcgatcggc cgatgaggac accgtgtcgt ttctcaccag gattttcggg gcgcgtacca
     4201 acgagcacta cacgcgcggc atcgtgcact tcaatcggca ggagtacgac aacgccgtcg
     4261 gcgagttcga gctggccgtg gccgagatcc aggaccacgc cgaccccgac tacgagctgg
     4321 cccgcttcta ctccgccgaa gctcacgcca agctgggcct ggcccgcctg cgcaccggcg
     4381 accgcgcgct cgccgaagcc gagttccgga aggccctcga ggtctcggac cagttcccgg
     4441 acctccacta tcacctgggc gtcctggccg agcaggacgg ccggccggac gaagccgaac
     4501 agcggctgcg ccacgcgctg gccatccacc cggactacgt cgaggcccgg gggtacctgg
     4561 ggctcacgct cctcggtcgc gggcgagccg acgaggcccg cgaggaaatc gccgaggcgg
     4621 cccggcgggg actgccgctt cccgatggct ttgcgctgat cccggcggcc gacgtgcccg
     4681 ccgcgctggt cgatgaactc cgcacccaga tcgagaaaca gtcgatcggc tcgatggtgc
     4741 tgtcgaacgc gctcgagagt tactcggttg ggcaggtcga agccgccatc accgagctcg
     4801 acgccgcggt caaggaacac cccacttatg ccgacctgcg ctgccggctt ggtcgcctcc
     4861 tggccgaaac cggcaagcac gaggaggcgc tggcccagtt cgccgccgcg ctcgagat
//
LOCUS       DASVOD010000347         5604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_76628, whole genome shotgun sequence.
ACCESSION   DASVOD010000347 DASVOD010000000
VERSION     DASVOD010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76628"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VF720_07885"
     CDS             complement(<1..551)
                     /locus_tag="VF720_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879314.1"
                     /translation="MAAGVYQLPLAAIAAAIIAFAAPVAAQAPDSSYWGAAPADSSPV
                     VLSQPGMPVWEGAIVYPWRVITYPIKLVTMGIGELVVAADESKLVGSVADLLSPPPSV
                     IGLSPTLAAGGLNGFGGGLTLYDNEFHHPDNRARLGFALTTNGSQLYRAGVLVNEPGR
                     TSLEIGGSYRLRRNARFFGVGPDT"
     gene            550..3285
                     /locus_tag="VF720_07890"
     CDS             550..3285
                     /locus_tag="VF720_07890"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7879315.1"
                     /translation="MLASLWRLLRIDRNEIPLLNSLFWLHFLVVASFTLAKAARDTLF
                     LEQWPAQILPWVNIVLVAWTLVSLAGHQRLTRGRTARPALGYTLSGVGLSMLGFYLLP
                     ASIAAPTAMLLYVWGATAGLILISQFWAVAEEATDPRSARRLYSVISAGGILGGFTGG
                     LAAALLGRIMGGHGPLLAAALVMLASAPVASHQPVRAPTPVLPPPQADAGKTPREVLK
                     HPYVQALALLMLVSGMASAVLDFKFKLAIQSMMSSGPELAVFLGNFYAVQNLAAFLLQ
                     VVFGGWLLSNLGARWTASLLPIGVAAASFGGLFLPVYPLVAVARLYDAVMRVSVTSTA
                     TEFFFFPMADDFRRKVKLLIDSVVSRISDALGALLLVAIGAFLPMDSKVLWWLLIAIT
                     IAWLGLLMRVGQLYVTELSQSLRRMVMAPPTNQTAVRESRLNSEIRVMLNNPSPERAL
                     YALSLLEESDPSAALEKVPELLKHPAAAVRRRAIELALEKPDVVDLLDMEALLHDDHD
                     EVRVWAALFFSKLTPGSPIDNLEELLESPTPRVRGSALHVVVQHARPDEAERVVALVR
                     RFLERDDPADRLAVAQVSGLPEAPAGLAPFLDPLIRDPDPRVRQAAMTAAARTGRRSA
                     IPLLLAALGRRGDRTTARAALAAWGERIVGTLGDHLIDDDFDPLLRRQIPRVLAQIAS
                     QDAVNNLLRVPLHKDRLLDLQVVKALNRIRAASRPVVFPDGPVTEQIQSEARQITVRL
                     VHRQAVTLIPAGRGRDLLLKALDERIDAGQDRLFRRLALLYPPREIHLAFLGLAASSL
                     KTRAQSVEYLASTLAPSERMSVLPLVDLLPDEERVNNAASFFRFQPRTPAEALTELAT
                     GPDAWLAACALFVIGEARLGELGSLIRRGVESTEPVVQDTARWADRRLHGFPAAG"
     gene            3374..3790
                     /locus_tag="VF720_07895"
     CDS             3374..3790
                     /locus_tag="VF720_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX7879316.1"
                     /translation="MDSGFFPGLSTEDLARVAGRTTEVHVEAGQLIDYDRGIHLLIEG
                     KVEVTLNDRVIRNIAPGDGIGLASVLGIEEHEGFAARAAEHSHLLSMCRDDFQDVLTE
                     FPEVAILLLKENMRQLLGLFRRIQELTGERPDDMPA"
     gene            complement(3841..5337)
                     /locus_tag="VF720_07900"
     CDS             complement(3841..5337)
                     /locus_tag="VF720_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX7879317.1"
                     /translation="MSLRPSGPTRRTATPVAAALVLASFAAVAHAQSPTYEDQVMEIV
                     NQERWTNGQLPPLKRNDLLNASATTHSSNMAVRNFFAHCDLDTGTKPWDRMTAAGYTG
                     WNYAAENIAAGYSTPAAVMIGWMNSAGHKANLLSTSSREIGIGYLMQSGDQSNVRGDS
                     NGDCAADGSPGGPYTRYWTQNFGRRNSIYPVVVNREAFQATEGLVSLYVYGTGWATQM
                     RFRNESGTWSAWLPYTENTTWVLSAGNGTKTVNCEIKNATGSVQANSDQIQLNASADV
                     AVNMTSGSATVESGQTWSYTLTTTNNGGSTANNILLSDALPAGLAYESANKPVTVTGN
                     TVTRTITTLGSGASDVVTITLRASGAAGAKVIPVNALAGEFDPVMANNTDSLPLDVTV
                     SSVGVPGDDPYAGAAVLALAPNLPNPFTTATRLAWTQPASAHVTLTVHDAAGRRIVTV
                     ADGRFDAGDHTVLWNGADADAKPVAAGLYFSRLVVDGQVRTGKLLLTR"
BASE COUNT          820 a         1903 c         1907 g          974 t
ORIGIN      
        1 gtgtccgggc cgacgccgaa gaagcgggca ttgcgccgca gccggtagct gccgccgatt
       61 tcgaggctgg tccggcccgg ctcgttgacg agaacgccgg cccggtaaag ctgcgagccg
      121 ttggtggtga gcgcgaagcc aaggcgcgcg cggttgtccg ggtgatggaa ctcgttgtcg
      181 tagagcgtga gcccgccgcc gaagccgttc aggccgccgg ccgccagcgt cggcgacaat
      241 ccgatcacgc tcggcggcgg cgagaggaga tcggcgacgg atccgaccag cttgctctca
      301 tcggcggcca cgaccagctc gccaatcccc atggtgacca gcttgatcgg gtaggtgatg
      361 acccgccacg ggtagacgat ggcgccttcc cataccggca ttcccggctg gctcaggacc
      421 accggggagg aatcggcggg agcggcgccc cagtagctgg agtcgggggc ctgcgcggcg
      481 acgggcgccg cgaaggcgat gatggcagcc gctatcgcgg ctagaggcag ctgatacact
      541 ccggcggcca tgctcgcatc actctggcgt ctcctcagga tcgaccgcaa cgagatccct
      601 ctcctcaaca gcctcttctg gctccacttt ctcgtggtgg cgtcgttcac gttggccaag
      661 gcggcccgcg ataccctgtt tctcgaacag tggccagccc agattctgcc gtgggtcaac
      721 attgtcctgg tggcctggac gctcgtgtcg ttggccggcc accagcgcct gacacgcggg
      781 cggacggccc gaccggccct cggctacacg ttgagcggcg ttggcctctc gatgctcggg
      841 ttctacctgc tccccgcctc cattgccgcg ccgaccgcga tgctcctgta cgtgtggggc
      901 gccacggccg gcctcatcct catttcccag ttctgggcgg tggccgaaga agccaccgac
      961 ccccgttcgg cccggcgact ctacagtgtc atcagcgcgg ggggaatcct cggcggtttc
     1021 acgggtggcc tggcggccgc cctgctcggc cggatcatgg gcggtcatgg ccccctgctg
     1081 gcggcggccc tcgtaatgct ggcctcggcg ccggtggcgt cgcaccagcc ggttcgggcc
     1141 cccacaccgg tcctgccgcc accccaggcc gacgccggca agacgccgag agaggtcctg
     1201 aaacacccgt acgtgcaggc cctggccctc ctcatgctcg tttccgggat ggcctcggcg
     1261 gtgctcgact tcaagttcaa gctcgccatc cagtcgatga tgtcgagcgg ccccgaactg
     1321 gccgtcttcc tggggaattt ctatgccgta cagaacctgg ccgccttcct cctccaggtg
     1381 gtattcggcg gctggctcct ctcgaacctg ggcgcccgct ggacggcgtc gctcctcccg
     1441 atcggcgtgg cggccgcttc gttcggggga cttttcctcc ctgtgtatcc gctggtggcc
     1501 gtggcgcgcc tctacgatgc cgtgatgcgc gtgtcggtca cctcgacggc caccgagttc
     1561 ttcttctttc cgatggccga cgacttccgc cgcaaggtga agctgctgat cgattcggtg
     1621 gtgagtcgca tttccgatgc gctcggcgcg ttgctgctcg tggccatcgg cgctttcctg
     1681 ccgatggact cgaaggtcct ctggtggctc ctcatcgcca tcacgattgc ctggctcggc
     1741 ctgttgatgc gggttgggca gctctacgtg accgaactct cgcagtctct ccggcggatg
     1801 gtgatggccc cccccaccaa ccagaccgcc gtccgcgaat cacgcctcaa ttccgaaatc
     1861 cgggtgatgc tgaacaaccc ctcgccggag cgggcgctct acgccctctc gctcctcgag
     1921 gagagcgatc cctccgctgc gctggaaaag gtgccggagc tcctcaagca cccggccgcc
     1981 gccgtccggc gccgggccat cgaactggcg ctggaaaagc cggacgtcgt ggatctgctc
     2041 gacatggagg cgttgcttca cgacgatcac gacgaggtac gcgtgtgggc cgccctcttc
     2101 ttctcgaagc tcacgccggg cagcccgatc gacaacctcg aggagctcct tgaatcgccc
     2161 acgccgcggg tgcgcggaag cgccctccac gtggtggtgc agcacgcccg tcccgacgag
     2221 gcggaacggg tggtggcgct cgtccggcgc tttctcgaac gggacgatcc ggccgaccgg
     2281 ttggcggtgg cgcaggtgag cggccttccg gaagcgccgg ccggactggc gccgttcctc
     2341 gatcccttga tcagggaccc cgatccccgc gtgcgacagg ccgccatgac ggcggcggcc
     2401 cgcaccggcc ggcgaagcgc catcccactg ctcctcgcgg cgctgggtcg acggggggac
     2461 cggactaccg cccgcgcggc gctggccgcc tggggcgaac ggatcgtcgg cacgctgggc
     2521 gatcacctga tcgacgacga cttcgatccg ctgctgcgcc ggcagatccc gcgggtactg
     2581 gcccagatcg cctcgcagga cgccgtgaac aacctgctcc gcgtgccgct gcacaaggat
     2641 cgcctcctcg acctccaggt cgtgaaggcg ctgaaccgga tccgggccgc ctcgcgcccg
     2701 gtggtcttcc cggacggccc cgtgaccgag cagatccaga gcgaggcccg ccagatcacg
     2761 gtgaggctgg tgcaccgcca ggcggtgacc cttatccccg cgggtcgggg tcgcgacctc
     2821 ctgctcaagg cgctcgacga acgaatcgat gccgggcagg accgcctttt ccggcgtctg
     2881 gcgctcctct acccgccacg ggaaatccac ctggcattcc tcggcctggc ggcttcgagc
     2941 ctcaagaccc gggcccaatc ggtggagtac ctggcctcga cgctcgcgcc gagcgagcgg
     3001 atgagcgtgc tgccgctggt ggatctcctg ccggacgagg agcgggtgaa caatgccgcc
     3061 tcgttcttcc gcttccagcc ccggacgccg gccgaggccc tgaccgagtt ggccacgggc
     3121 cccgatgcct ggctggccgc ctgcgccctc ttcgtgatcg gcgaagcgcg gctaggcgag
     3181 ctgggttccc tcatccgtcg gggcgttgag tcgacggaac cggtggtgca ggatacggcg
     3241 cggtgggccg accggcgttt gcacggcttc cccgccgcgg gctgagcgca ttgatcatct
     3301 gcagagccga ctgaacccga ccggaggcac actggaaccc cgactgtcga tcatcgaacg
     3361 agccctgatc atcatggatt cgggcttctt ccccgggctc tccaccgagg acctggcgcg
     3421 cgtggccggt cgcacgaccg aagtgcacgt cgaggccggc cagttgatcg actacgaccg
     3481 cggcatccac ctcctgatcg aagggaaggt ggaagtgacg ctcaacgacc gcgtcatccg
     3541 gaacatcgcg ccgggcgacg gcatcggcct cgcctcggtg ctgggcatcg aggagcacga
     3601 gggattcgcc gcgcgggccg cagagcactc ccatctactt tcgatgtgcc gcgatgactt
     3661 ccaggacgtg ctgacggagt ttcccgaggt ggccatcctg ctgctcaagg agaacatgcg
     3721 ccagctgctc ggcctgttcc gccggatcca ggagctcacc ggcgagcggc ccgatgacat
     3781 gccggcctga tccccgcacg cctggctgac cagcaaccgc gggctacctc gtccgcttga
     3841 ttaccgcgtc agcaggagct ttccggtccg cacctggcca tccaccacca gccggctgaa
     3901 gtagaggccg gcggccaccg gcttcgcgtc cgcgtccgct ccgttccaga gaactgtgtg
     3961 atcaccggca tcgaaccggc catcggccac cgtcacgatc cgccgcccgg cggcgtcgtg
     4021 caccgtgagc gtcacgtggg ccgaagccgg ctgcgtccag gccagccgtg tggcggtcgt
     4081 gaagggattc ggcagattcg gagccagtgc cagcacggcg gcgcccgcgt acgggtcgtc
     4141 accgggcacg ccaaccgatg acacggtcac gtcgagcggc aggctgtcgg tgttgttggc
     4201 catgaccgga tcgaactcgc cggccagggc attgaccggg atgaccttgg cgccggccgc
     4261 cccggaggcc cgcagcgtga tggtcaccac gtcggaggcg cccgagccga gcgtggtgat
     4321 ggtgcgggtg accgtgttgc cggtcaccgt gacgggcttg ttggccgact cgtaggcgag
     4381 cccggccggc aacgcatcgg acagaaggat gttgttggcg gtggagccgc cgttgttcgt
     4441 ggtggtgagc gtatagctcc acgtctggcc cgactcgacg gtggccgagc ccgaggtcat
     4501 gttcacggcc acgtcggcgc tggcgttcaa ctggatctgg tccgagttgg cctgcaccga
     4561 gccggtggcg ttcttgatct cgcagttcac cgtcttcgtg ccgttgccgg ccgacagcac
     4621 ccaggtggtg ttctcggtat acggcagcca ggccgaccag gtgccgctct cgttgcggaa
     4681 gcgcatctgg gtggcccagc cggtgccgta cacatagagg ctcaccagcc cctcggtggc
     4741 ctggaatgcc tcgcggttca cgaccaccgg gtagatggaa ttgcgacggc cgaagttctg
     4801 ggtccagtaa cgggtgtacg gaccgcccgg agagccgtcc gcggcgcaat cgccattcga
     4861 atcgccccgg acgttcgact ggtcacccga ctgcatcagg tagccgatgc cgatctcgcg
     4921 gctgctggtg gagaggaggt tggccttgtg cccggccgaa ttcatccagc cgatcattac
     4981 cgcggccggc gtggagtacc cggccgcgat gttctcggcc gcgtagttcc aaccggtgta
     5041 cccggcggcc gtcatccggt cccacggctt ggtgccggtg tcgagatcgc agtgagcgaa
     5101 aaaattgcgc accgccatgt tgctgctgtg ggtggtggcc gacgcgttca ggaggtcgtt
     5161 ccgcttgagc ggggggagct ggccgttggt ccagcgctcc tggttcacga tctccatcac
     5221 ctggtcttcg taggtggggc tctgggcgtg ggcgacggcg gcgaacgagg cgagaacgag
     5281 ggcagctgct actggcgtgg cggtacggcg ggtgggaccg gaggggcgga gcgacatgac
     5341 caacatctcc cgtgacgcgg ctcatctggg tggagtgaaa ctctgtgcgc ggtcacagaa
     5401 atcttcggtt gggccgtcgg caccttaaga gttttcccga tgcatgggaa gcgaaaagat
     5461 gataaggggg ccgactccgg gtggttttca tcaccgtggt gcaggatgcg cggcccctgt
     5521 tgcagttcga agcacgtcgg acggtcagga gtgcccgggc ccctcgtggt ggttcgcgag
     5581 gacacgctcg atccggcggt cggg
//
LOCUS       DASVOD010000348         8761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_25597, whole genome shotgun sequence.
ACCESSION   DASVOD010000348 DASVOD010000000
VERSION     DASVOD010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8761
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25597"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VF720_07905"
     CDS             <1..1585
                     /locus_tag="VF720_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879318.1"
                     /translation="DAVGKARPGSNAPADPRYNQLMRNAARQASQGQHLLASRSYEQI
                     LTTWPGDINATRGCADELVALGQLQSAEKLLTDWRREHGADVGIERSLATVHRAEERW
                     DDYLSDVLRALDDPGPGQEPPVSWALRSLEEIGNQPSMSTKVEPAVKKLVRDNPDQPQ
                     LRILLADQLLRNGHEAEALAEVEAADRAAKAGGKLIAEFGEELDVQGKSVLAESAFRK
                     AAESADSQPARIAAWMRLGELAERNEHPKVQVEAWQAIVTLAPQSPEALAALQSLADA
                     QLYRLNDHAAALATLQKLEANPAMAERKGEIYLPMAECQFRLDQAEAALTTLERLKGT
                     KADVETQAEGAFLAAEVRFYGGEFDDAQPAYQAVAENFTRTRKTNDAVGRYLQIARAK
                     DQGDLEALRAYATMEKFNRMADTTATWNAATTLLSKFAASDLAADAMVRQAEILRAKP
                     GQSEDAIALCAKAVEEHPKARMAAYALAVMGDICLKDLNDKKRALAAYERLLDEYPQN
                     LLAAEVRRTVERLRKNNES"
     gene            1674..2852
                     /locus_tag="VF720_07910"
     CDS             1674..2852
                     /locus_tag="VF720_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HEX7879319.1"
                     /translation="MDLTQTNHLKAYGLAYWALLNGETVEWLLNYRGGSFLFANGSGA
                     TREARIRGVSYETIDDGAAGAIYREIESSNMELVLLEKAPRMAVYIPPGFVPWDDAVT
                     LALTYADIPFTQIWDEQVLNGDLSKYDWLHLHHEDFTGQYGRFYANFRNHDWYRQQQA
                     TYEADAKRLGFSKVSECKKAVARAIREYVAQGGFMFAMCSATDSYDIALAAEGVDICE
                     SMYDGDPADPTAQSKLDFSKCLAFTNFILEMNPLVYEFSNLDTTNQSQARGPEADFFT
                     LFDFSAKEDPVQTMLTQCHTNVIPGFMGQTTGFRRDLIKKGIILMGQVEGTREVKYLN
                     GNFGQGTFTFYGGHDPEDYQHQVGDPPTDLSLHPNSPGYRLILNNVLFPAAEKKKHKT
                     "
     gene            2852..4240
                     /locus_tag="VF720_07915"
     CDS             2852..4240
                     /locus_tag="VF720_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879320.1"
                     /translation="MPSVRRAPRSPFVRAAGMGALAAILLCGAASACAGTWVYDHLSW
                     NPGGDTLGVMAWSDPPGGDQPGRRLYIPVDTAQALTVAPDVRVVQVSPDGTAALLLGD
                     GGLWWYGATTGRLVQLRYQSPASGERVYDMGFRKGDDGVNFATGAPGDRTGAWYRWMP
                     GKPVERTGARTPGAAMDTAWTNQTLPAMRPNVGAMRSARRVTVPGSPSIVMDYDSKDL
                     VVATGYGGDRAKLEDVEVEWIVWPQEGARALIALRHRGDGRPELISVSGGGFHPLGSR
                     GAAFGGWLADGSALLADADGRLVSWTPLTDSLAVVPLDEPPRWARYLSRDATVLSLAV
                     TRWPRSARRFESQVELVKKAAATLPEEWRAGAMAYGRRLLGGDQRVGVFPTDEAARRA
                     ADAFHASGMTVTPVTSTAREMTGAFDYALTRNGPGDAAWIRRLDTGGAEVWLLRKAGG
                     QPIRLLTREMTR"
     gene            4237..5709
                     /locus_tag="VF720_07920"
     CDS             4237..5709
                     /locus_tag="VF720_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879321.1"
                     /translation="MIERHRRPARSTRAIPSWFALAAALALACLSAPADATWTPQYFI
                     FDRMSWSPDGKSLGVIGRYWNKVTGESFEDTLLVDPATGKIRCVSPSALEAVISRDGS
                     RLLARGRWGLYDHDLKSGQTRELMTQHPFQPIQIVHFSYDREMGAAMVIRCNDWDPTV
                     NGVYRLPLDGADPVKLMSDELCGPSLLNYFQTHTRGIYSVRRAEPGMGHPVSPANFPG
                     TIWQVETEGVPEVAVWGNGAAGSDTLCHDCRPEFLSWPKEGATLLMGTCPVELSDIVG
                     SGQLWLIRPGQEAKSLGAGRFDACVWADSSHAYVISRPSALEAVDVARGESRQVNLSM
                     TPGWLKDKILVPPRVWTVRLAGDIHTTADSAGASFRGAAELLGGARGTGWSLLRQDDP
                     KTFEITVGSFDDSASAVAAARKLAAAKYRGVNDFPVLRRPVFDMLGDFQFGSISAPGG
                     AYRAWFRTHPHLVQPYMSTELWIEKKGERPRPVLEGMGTF"
     gene            5834..6025
                     /locus_tag="VF720_07925"
     CDS             5834..6025
                     /locus_tag="VF720_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879322.1"
                     /translation="MEKAFPECQKCHAGVLLPLSDYGRDGAPIQYKAWVCSNPPCGFN
                     VRIDNGEISIGRPITTSNK"
     gene            6072..6770
                     /locus_tag="VF720_07930"
     CDS             6072..6770
                     /locus_tag="VF720_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX7879323.1"
                     /translation="MIQAIIFDLDNTLTDFMTMKDLAIRGAAEAMIDAGLKKSPDDAR
                     DEINAIYREMGIEYQKVFDVYLTRTLGQIDYKMLAAGIVGYRRARDSALILYPHVHLT
                     LVELIKRGLKLAVVSDAPREQAWLRLCYLKLQHMFDVVVTFEDTLKRKPSPEPFQRAL
                     DQLGVPAERTLMIGDWPERDVLGAKGLGMTTVFARYGDTFGTPPGQSGADHDIGDVMD
                     LLDIVDRLNGTHGH"
     gene            6760..7239
                     /gene="acpS"
                     /locus_tag="VF720_07935"
     CDS             6760..7239
                     /gene="acpS"
                     /locus_tag="VF720_07935"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX7879324.1"
                     /translation="MDTEARGMQSLGIDIVEVGRIREMINRFGDRFMRKIFTDHEIAT
                     CMAKARPSESFAARFAAKEAFAKVWPGPVLPGWRDVEVLMEGPRPTFRFDGLAKGSVA
                     GLSISHTHSYAAAVVWLVSAPVGVSAAAEVPAATEIPASPHPPAPPRRRGPPDSGFR"
     gene            7259..>8761
                     /locus_tag="VF720_07940"
     CDS             7259..>8761
                     /locus_tag="VF720_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX7879325.1"
                     /translation="MIPLVRAAQMREIDRTAIQEMGVGSLDLMETAGRAVAEAVLAHF
                     GAEEARRVLVVCGKGNNGGDGLVAARYFAAAGCRPRVVVIGSEAELSDDARAMLARWT
                     ATGGATEFLPDDGAVMRFFQNPPACDVAVDALLGTGATGQPRGPVLEAIRGLNRLGRP
                     IAAIDIPSGIDADSGAVPGAAVDADLTVTLGLPKRGLWLYPARERAGVVEVADIGLPR
                     EASLSADVRDFLVQDEDAPAWLPAWPRNAHKGTRGRLLIVGGSEGLTGAPCLAAGAAL
                     RVGAGLVTVGAPSGLVDILAAKLTEAMTLALPQAPARCLAPEAMAAVESFDTVRLSAA
                     VLGPGLSRREPAAAFARAMVAAFDRPMVVDADALHAFENQPDLLARRRAGTTTGAAGP
                     LVLTPHPGEAAWLMGQPAHAIDVGRIDLAREWSARLGQVIVLKGAPTVIGSPEGEAFV
                     NPTGGPLLATGGTGDVLAGLIGGLLAQGASPLNAALLGVWLHGFLADLFEE"
BASE COUNT         1416 a         2991 c         3042 g         1312 t
ORIGIN      
        1 agacgcggtg gggaaggccc gacccgggag caacgcgccg gccgatcctc gctacaacca
       61 gctgatgcgg aacgccgcgc gtcaggccag ccagggacag cacctgctgg cgtcgcgctc
      121 gtacgaacag atcctcacca cctggcctgg cgacatcaac gccacccgcg gctgcgcgga
      181 tgagctggtg gcgctgggcc agctccagtc ggccgagaaa ctcctgaccg actggcggcg
      241 tgagcatggc gccgacgtgg gcatcgagcg cagcctggcc acggtgcacc gggccgagga
      301 gcgctgggac gactacctgt ccgacgttct ccgggcgctg gacgatcccg gcccggggca
      361 ggagccgccg gtttcctggg cgctgcgttc gcttgaagag atcggcaacc agccgtccat
      421 gagcaccaag gtcgaacccg cggtcaagaa gctcgtccgc gacaacccgg accagcccca
      481 gctccgcatc ctcctggccg accagcttct ccggaacggt cacgaggcgg aggcgctcgc
      541 cgaggtcgag gcggccgacc gtgccgcgaa ggcgggcggc aagctgatcg ccgaattcgg
      601 cgaggagctc gatgtccagg ggaagtcggt gctggccgaa tcggccttcc ggaaggccgc
      661 cgagtcggcc gattcccaac cggccaggat cgcggcctgg atgaggctcg gcgagctggc
      721 cgaacggaat gaacatccga aggtgcaggt ggaggcctgg caggccatcg tcacgctggc
      781 gccgcaatcg cccgaggcgc tggcggcact gcagtcgctg gccgatgccc agctctaccg
      841 gctgaacgat catgccgccg ccctggccac gctgcagaag ctcgaggcca atccggccat
      901 ggccgagcgg aagggggaga tctatctccc gatggccgaa tgccagttcc ggctcgatca
      961 ggccgaagcg gctctcacca cgctggagcg gttgaagggg acgaaggccg acgtggagac
     1021 gcaggccgag ggggcgttcc tggctgccga agtccgcttc tacgggggcg agttcgacga
     1081 cgcgcagccg gcctaccagg cggtggccga gaacttcacc cgcacgcgca agaccaacga
     1141 cgcggtgggg cgctacctcc agatcgcccg ggccaaggac cagggcgatc tcgaggcgct
     1201 gagggcctac gccacgatgg agaagttcaa ccggatggcc gacaccacgg ccacctggaa
     1261 cgcggccacc acgctccttt cgaaattcgc cgcgagcgac ctggcggccg acgccatggt
     1321 ccggcaggcc gaaatcctcc gcgcgaagcc ggggcagagc gaggatgcca tcgccctgtg
     1381 cgccaaggcg gtcgaggaac atccgaaggc acggatggcc gcgtacgcgc tggccgtgat
     1441 gggcgacatc tgcctgaaag acctgaacga caagaagcgg gcgctggccg cctacgaacg
     1501 gctgctcgat gaatacccgc agaacctgct cgcggccgag gtgcggcgca cggtggagcg
     1561 cttgaggaag aacaatgaat cgtagtttac ggcagttcct tgccgcgctg ctcgcggggc
     1621 tggcgttcgt catcggaggg gcttcgggcg cctcggcccg cacgctcgtg ccgatggatc
     1681 tcacgcagac caatcacctc aaggcctacg gcctggccta ctgggcgctc ctcaacgggg
     1741 aaaccgtgga gtggctcctc aactaccgcg gcggcagctt cctgttcgcg aacggcagcg
     1801 gcgccacgcg cgaggcgcgc atccgcggcg tgtcgtacga aacgatcgac gacggtgcgg
     1861 ccggcgccat ctaccgcgag atcgagtcga gcaacatgga gctggtgctc ctcgagaagg
     1921 ccccccgcat ggccgtgtac atcccgcccg gcttcgtgcc gtgggacgac gcggtgacgc
     1981 tggcgcttac ctatgcggac atcccgttca cgcagatctg ggacgagcag gtgctcaacg
     2041 gcgatctctc caagtatgac tggctccatc tccaccacga ggatttcacc ggccagtacg
     2101 gacggttcta cgccaacttc cgcaaccacg actggtaccg tcagcagcag gccacctacg
     2161 aggccgacgc caagcggctc ggcttcagca aggtgagcga gtgcaagaag gcggtggccc
     2221 gggccatccg cgagtacgtg gcgcagggtg gcttcatgtt cgccatgtgc tcggccaccg
     2281 acagctacga catcgcgctg gcggccgaag gggtggatat ctgcgagtcg atgtacgacg
     2341 gcgacccggc cgacccgacg gcgcagtcga agctcgactt ctcgaagtgc ctggccttca
     2401 ccaatttcat cctcgagatg aaccccctcg tgtacgagtt ctcgaacctg gatacgacga
     2461 accagagcca ggcccgcggc ccggaggccg acttcttcac gctgttcgac ttctccgcca
     2521 aggaagaccc ggtccagacg atgctcaccc agtgccacac caacgtcatc cccggcttca
     2581 tggggcagac gaccggcttc cgcagggacc tcatcaaaaa ggggatcatc ctgatggggc
     2641 aggtggaggg aaccagggaa gtgaagtacc tgaacggcaa cttcggccag gggaccttca
     2701 cgttctacgg cggccacgac cccgaggact accagcacca ggtgggcgac ccgccgaccg
     2761 acctgtcgct gcaccccaac tccccgggct atcgcctcat cctcaacaac gtgctcttcc
     2821 cggcggcgga aaagaagaaa cacaagacct gatgccctcc gttcgccggg ctccccggtc
     2881 gccgttcgtc cgcgcggccg gcatgggcgc gctcgccgcg atactcctct gcggcgcggc
     2941 ctcggcctgt gcgggtacct gggtgtacga ccacctgtcg tggaatcccg gcggcgacac
     3001 gctcggcgtg atggcctgga gcgacccgcc gggcggcgac cagccggggc gacgcctcta
     3061 catcccggtg gacacggcgc aggcgctgac ggtggcgccc gacgttcggg tcgtgcaggt
     3121 ttcgcccgac ggcaccgccg cgctcctgct cggcgacggc gggttgtggt ggtacggcgc
     3181 gacgaccgga cgcctcgtcc agctccgcta ccaatcgccg gcctcgggcg agcgcgtgta
     3241 cgacatgggg ttccggaagg gggacgacgg cgtgaatttc gcgacgggag cgccgggaga
     3301 ccgcacgggc gcctggtatc gctggatgcc ggggaagccg gtggaacgca ccggcgcgcg
     3361 cacgccgggg gcggccatgg acaccgcctg gacgaaccag accctcccgg ccatgcgccc
     3421 gaacgtcggc gcgatgcgct cggcccgccg ggtgaccgtg ccgggctcgc cctcgatcgt
     3481 gatggactac gactcgaagg atctcgtcgt ggccaccggc tacggcggtg accgcgcgaa
     3541 actggaagac gtggaggtgg agtggatcgt ctggccgcag gagggggcgc gggccctgat
     3601 cgcgctccgc caccgggggg acggccgtcc ggaactcatc tcggtgtcgg gcggtggttt
     3661 tcatccgctc ggctcgcgcg gcgcggcatt cggcggatgg ctcgccgatg gctcggcgct
     3721 cctggccgac gccgatggcc gcctcgtctc ctggacaccg ttgaccgatt cgctggccgt
     3781 cgtgccgctc gacgagcccc cgcggtgggc ccgctacctc tcgcgcgatg ccacggtcct
     3841 ttcgctggcc gtgacgcgct ggccgcgctc ggcccggcgg ttcgagagcc aggtggagct
     3901 ggtgaaaaag gccgccgcca cccttcccga ggaatggcgg gcgggggcca tggcctacgg
     3961 ccgccggctc cttggagggg accagcgcgt gggtgtgttt cccacggatg aggcggccag
     4021 gcgggccgcc gacgcgtttc atgccagcgg catgacggtg acgcccgtga cgagcaccgc
     4081 gcgcgagatg acgggcgcct tcgattacgc actcacccga aacggaccgg gcgatgccgc
     4141 ctggatccgg cgactcgata ccggcggtgc cgaagtgtgg ctcctccgga aagcgggcgg
     4201 ccagccgatc cgactcctca cccgggagat gacccgatga ttgaacgaca ccgccggccc
     4261 gcacgatcca cccgtgccat tccatcctgg tttgcgctcg cggccgcact ggcactggca
     4321 tgcctcagcg cacccgcgga cgccacctgg acgccgcagt acttcatctt cgatcgcatg
     4381 tcctggagcc cggacggcaa gtcgctcggc gtgatcgggc ggtactggaa caaggtgacc
     4441 ggcgagtcgt tcgaggacac gctcctggtc gacccggcca ccgggaaaat ccgatgcgtt
     4501 tccccgtccg cgctcgaggc ggtgatctcg cgcgacggtt cgcgcctcct ggcccgggga
     4561 cgctggggtc tctacgacca cgacctgaag agcggccaga cgcgtgagct catgacccag
     4621 cacccgtttc agccgatcca gatcgtccac ttctcatatg accgcgagat gggcgccgcg
     4681 atggtcatcc ggtgcaacga ctgggatccg acggtgaatg gcgtctaccg ccttccgctc
     4741 gacggggccg acccggtcaa gctgatgagc gacgaactgt gcggaccctc gctcctcaat
     4801 tactttcaga cgcacactcg agggatctat tcggtgcggc gggccgaacc cggcatgggt
     4861 cacccggtca gtccggccaa tttccccggg acgatctggc aggtggaaac cgagggggtg
     4921 cccgaggtgg ccgtctgggg aaacggtgcg gcgggcagtg acacgctctg ccacgactgc
     4981 cgcccggaat tcctctcctg gccgaaagag ggggccacct tgctcatggg gacgtgcccg
     5041 gtcgagctct ccgacatcgt cggttccggg cagctgtggc tgatccgccc cggccaggag
     5101 gcaaagagcc tgggcgccgg tcgattcgat gcgtgcgtct gggccgactc gagccacgcc
     5161 tacgtcatca gccgaccgag cgctctcgag gccgtggacg tggcgagagg agagtcgcgg
     5221 caggtgaacc tttcgatgac gcccggttgg ctgaaggaca agatcctcgt cccgccccgg
     5281 gtgtggaccg tgcggctggc cggcgacatc cacaccacgg ccgattcggc cggcgcctcg
     5341 ttccgcgggg cggccgagct cctgggcggc gccaggggga cagggtggag cctcctccgg
     5401 caggacgatc cgaagacatt cgagatcacg gtgggttcgt tcgacgactc ggcctcggcg
     5461 gtggcggcgg cccggaagct ggctgccgcc aagtatcgcg gggtcaatga cttccccgtg
     5521 ctcaggcgcc ccgtcttcga catgctgggc gacttccagt tcggctcgat cagcgcgccg
     5581 ggcggcgcgt accgggcctg gttccgcacg catccccacc tggtccagcc ttacatgtcg
     5641 acggaactgt ggatcgagaa gaaaggagaa cgcccgcgtc ctgtactcga gggaatggga
     5701 acgttctagt cccctgagct cccctacttg acacctcgtc gcagggcgtt cgaaactggc
     5761 tcgagaacgc catcttccgc cggccaggag aacgggccgg acgtgaagga ggacggggag
     5821 ggggggcgat ccaatggaaa aggcgtttcc ggagtgccag aagtgccatg ccggggtcct
     5881 gttgccgttg tccgattacg gccgcgatgg agccccgatc cagtacaaag cctgggtctg
     5941 ttcgaacccg ccctgcgggt tcaacgtgcg gatcgacaac ggcgaaatct cgatcggccg
     6001 tccgatcacc acgagcaaca agtagccgcc gccggccggg cggtccagcc gccccccgcg
     6061 ggagccgcgt catgatccag gccatcatct tcgacctcga caataccctg accgacttca
     6121 tgaccatgaa ggacctggcc atccggggtg cggccgaggc gatgatcgac gccggcctca
     6181 agaaatcgcc cgatgatgcc cgcgacgaga tcaacgccat ctaccgggag atggggatcg
     6241 aataccagaa ggtgttcgat gtctacctca cccgcaccct cgggcagatc gattacaaga
     6301 tgctggcggc cgggatcgtc ggctatcgcc gggcgcgcga ctcggcgctc atcctctacc
     6361 cgcatgtgca cctcacgctc gtcgagctga tcaagcgggg gctgaagctg gccgtggtga
     6421 gcgatgcgcc gcgtgagcag gcatggctgc ggctctgtta cctcaagctg cagcacatgt
     6481 tcgacgtggt cgtcaccttc gaggacacgc tcaagcgcaa gcccagcccc gagccgttcc
     6541 agcgggcgct cgaccagctc ggcgtgcccg cggaacgaac cctgatgatc ggggactggc
     6601 ccgaacggga cgtgctcggc gccaaggggc tgggcatgac caccgtgttc gcgcgctacg
     6661 gagacacgtt cggcacgccg cccgggcaat cgggcgccga ccacgacatc ggcgacgtga
     6721 tggacctgct cgacatcgtc gaccggctga acggcacgca tggacactga ggcgcgcggg
     6781 atgcagtcgc tggggatcga catcgtcgag gtggggcgca tccgcgaaat gatcaaccgc
     6841 ttcggcgacc gcttcatgcg aaagatcttc accgaccacg agatcgccac ctgcatggcc
     6901 aaggcgcggc cgtccgagtc gtttgccgcc cggttcgccg cgaaggaggc ctttgccaag
     6961 gtgtggccgg ggcccgtgct gccggggtgg cgcgatgtgg aagtcctgat ggaggggccg
     7021 cgcccgacct tccgtttcga tggactggcg aaagggagtg tggcgggcct gtcgatcagt
     7081 catacccact cttacgcggc ggcggtcgta tggctggtgt cggcgccggt cggtgtatcg
     7141 gctgcggccg aagttccggc ggcgacggag atcccggcat cgcctcaccc accggcgcca
     7201 ccacgtcgcc gggggccgcc cgactcgggt ttccgatgac gcaccggatc accttccgat
     7261 gatcccgctg gttcgcgcgg cgcagatgcg cgagatcgat cgcacggcca tccaggagat
     7321 gggtgtcgga tccctcgacc tgatggagac ggccggccgc gccgttgccg aggcggtgct
     7381 cgcccacttc ggggccgagg aagcgcgacg ggtcctcgtc gtatgcggga aggggaacaa
     7441 cggcggcgac ggcctcgtgg cggcacgata tttcgcggcg gcggggtgcc gtccgcgcgt
     7501 ggtggtgatc ggcagcgagg cggagctctc cgacgacgcc cgggccatgc ttgcgcgctg
     7561 gacggcaacg ggaggcgcca ccgaattcct tccggacgac ggggcggtca tgcgcttctt
     7621 tcagaatccg ccggcctgcg acgtggccgt ggacgcattg ctcggcaccg gcgccacggg
     7681 ccagccacgc ggcccggtgc tcgaggccat ccgcggcctc aaccgcctcg gccggccgat
     7741 cgcggccatc gacatcccga gcggtatcga tgccgactcg ggcgcggtgc ccggcgcggc
     7801 cgtcgacgcc gacctgaccg tcacgctcgg actgccgaaa cgggggctct ggctctaccc
     7861 ggcccgcgag cgagcgggcg tggtggaggt ggcggacatc ggcctgccgc gcgaggccag
     7921 tctttccgcc gacgtgcgcg acttcctggt ccaggacgag gacgcgccgg cctggctccc
     7981 cgcctggccg cgcaatgccc acaagggaac gcgcggccga ttgctcatcg ttgggggctc
     8041 ggaagggttg accggggccc cctgcctggc agccggagcg gcactgcgcg tcggtgcggg
     8101 gctcgtcacc gtcggcgctc cctccggact ggtcgacatc ctggccgcga aattgaccga
     8161 ggcaatgacg ctggccctgc cgcaggcgcc ggcgcgctgc ctggctcccg aggcgatggc
     8221 cgccgtcgag tcgttcgata ccgtgcgcct ttcggccgcg gtcctcgggc ccggtctctc
     8281 gcgtcgcgaa ccggccgcgg cgttcgcacg ggccatggtg gccgcgttcg accggccgat
     8341 ggtggtggat gccgacgcgc tccacgcctt cgaaaaccag ccggacctcc tcgctcgccg
     8401 gcgtgcgggc accacgaccg gcgcggccgg tcccctcgtg ctcacgccgc atcccggcga
     8461 ggccgcctgg ctgatgggac aaccggccca cgccatcgac gtgggccgga tcgacctggc
     8521 ccgcgagtgg tcggcccggc tcggccaggt gatcgtgctg aaaggggctc ccaccgtgat
     8581 cggatcgccg gaaggggagg cgttcgtgaa cccgacgggc gggccgctcc tcgccaccgg
     8641 cggcaccggc gacgtactgg ccggcctgat cggcggcctt cttgcccagg gcgcctcacc
     8701 cctcaatgcg gctctcctcg gtgtgtggct tcacggcttc ctggccgacc tcttcgagga
     8761 g
//
LOCUS       DASVOD010000349         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_128919, whole genome shotgun sequence.
ACCESSION   DASVOD010000349 DASVOD010000000
VERSION     DASVOD010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128919"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VF720_07945"
     CDS             complement(<1..516)
                     /locus_tag="VF720_07945"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX7879326.1"
                     /translation="MSRLRERVDAKLYYSISEASAIAKVKPHVLRYWETQFKMLRPKK
                     NKAGNRMYRKRDLKLIQLIKELLYDKGYTIAGARRKLLDDRAALRDQLEIKFTDAERS
                     TLLADIIRDLKVVLEDLKRLPGEAARRSEAEEAISEAREAAARTDVGTDSANGADGLD
                     GADGADSPDGSD"
     gene            complement(536..1528)
                     /locus_tag="VF720_07950"
     CDS             complement(536..1528)
                     /locus_tag="VF720_07950"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX7879327.1"
                     /translation="MIVGVLGLGSWGTALATLLAGRFEVAGWTHDPAQRTRLRAEREN
                     KKYLPGVMLPSNLRIEDTLAGVLAGCALVVFAVPSHALREVAMAASPHLPSGITVVNV
                     AKGLEEGSLDRMSSVLMEVLPAGTPIVSLLGPSHAEEVSRQQPTALVAASHDPAAARR
                     TQEVFNGETLRIYTSPDLVGVELAAGLKNIIAIAAGIAVGVGYGDNTMGALVTRGLAE
                     MTRLGTNLGGRPETFSGLSGLGDLVTTCISTHSRNRWVGEQLGKGRPAAEVLGSMVMV
                     AEGVRTTHAGVALAARHGVELPIAAAVKSVLDGTRDPRQAIRDLMLRDPKAELA"
     gene            complement(1525..2154)
                     /gene="plsY"
                     /locus_tag="VF720_07955"
     CDS             complement(1525..2154)
                     /gene="plsY"
                     /locus_tag="VF720_07955"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEX7879328.1"
                     /translation="MNYYLAVILTGFLLGSMPWGLWMGHLLGRGDIRKSGSGNLGATN
                     VYRTMGAWAALLVLLLDAGKGYIATAIVPGLLPGSESWPYLRLVGGLAAVCGHILTPF
                     ASFKGGKGVATSLGVFIGLAPVPTAISFGAFVVFVAATGFVSLGSMIAAVVLPVAIYL
                     TRATVQHQWGAVLILAVISATLILIRHRANLRRLMAGTESPLWKKGPKA"
     gene            complement(2151..>2485)
                     /locus_tag="VF720_07960"
     CDS             complement(2151..>2485)
                     /locus_tag="VF720_07960"
                     /inference="COORDINATES: protein motif:HMM:NF026064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX7879329.1"
                     /translation="IPALARQLARERKREISTSELNRFLTAMEQKYQPPASKRVGKPP
                     RIYYGTQTGTAPPVFTLFVNEPLAFAASYRKYVINSLRESLGFEGSPIEVRYKARRPT
                     KGSGDSRR"
BASE COUNT          386 a          806 c          887 g          406 t
ORIGIN      
        1 atcggagcca tccgggctgt cggcgccgtc ggcgccgtcc agtccgtcag cgccgttggc
       61 gctgtccgtg cccacgtcgg tgcgggccgc ggcttcgcgg gcttcggaaa tggcctcctc
      121 ggcctcggaa cggcgggccg cctcgcccgg caggcgcttc aggtcctcga ggacgacctt
      181 gaggtcgcgg atgatgtcgg ccaggagcgt ggagcgctcg gcgtcggtga acttgatctc
      241 gagctggtcg cgcagtgcgg cccggtcgtc gagcagcttc cggcgggcgc cggcgatcgt
      301 gtaccccttg tcgtacagca gctccttgat gagctggatc agtttcaggt cgcgcttgcg
      361 gtacatgcgg ttgccggcct tgttcttctt gggccggagc atcttgaact gggtttccca
      421 gtagcgcagg acgtggggct tgaccttggc gatggcgctc gcctcgctga tcgaataata
      481 gagtttcgcg tcgacgcgct cccggagacg actcatgacc acctcacaaa gcggctcatg
      541 ccagctccgc cttcggatcg cgaagcatga ggtcgcggat ggcctggcgc gggtcgcgcg
      601 tgccgtcgag gaccgacttc accgcggcag caatcgggag ttcgactccg tgacgcgccg
      661 ccagcgccac gccggcatgc gtggtgcgca cgccctcggc caccatcacc atgctgccga
      721 ggacttcggc ggccgggcgt ccctttccaa gctgctcccc cacccagcgg ttgcggctgt
      781 gcgtggagat gcaggtggtg acgagatcgc cgaggccgga gaggccgctg aacgtttcgg
      841 gacggccgcc caggttcgtg ccgagccggg tcatctcggc caggccccgg gtgacgagcg
      901 cgcccatggt gttgtcgccg tagccgacgc cgaccgcaat gcccgcggcg atggcgatga
      961 tgttcttgag gccggcggcc agttccacgc cgacgaggtc ggggctggtg tagatgcgga
     1021 gcgtctcgcc gttgaacact tcctgcgtgc ggcgcgcggc agccggatcg tgtgaggcgg
     1081 ccaccagcgc ggtgggctgc tgacgactga cctcctcggc gtgcgacggc ccgagcaggg
     1141 aaacgatcgg ggtgccggcc ggaagcactt ccatgaggac gctcgacatc ctgtcgagcg
     1201 atccttcttc caggcccttg gcgacgttga cgaccgtgat tcccgacggc aggtgcggcg
     1261 aggcggccat ggccacctcg cgtaaggcat gggacggcac cgcgaagacc acgagcgcgc
     1321 agccggccag gacgccggcc agtgtgtcct cgatgcggag gttcgacggg agcatcaccc
     1381 ccggaagata tttcttgttt tcgcgctccg cgcggaggcg cgtgcgctgg gcgggatcgt
     1441 gcgtccagcc ggccacctcg aaccgtccgg ccaggagcgt ggccagggcg gtgccccagg
     1501 agccgaggcc gaggacgccg acgatcatgc cttcggtccc ttcttccaga ggggcgattc
     1561 ggtgccggcc atgaggcgcc ggaggttcgc gcgatggcgg atcaggatga gcgtggccga
     1621 gatgacggcc aggatgagga ccgcccccca ctggtgctgt accgtggcgc gcgtcaggta
     1681 gatggccacc ggcaatacca ccgcggcgat catcgacccg agcgacacga accccgtggc
     1741 cgccacgaag acgacgaacg cgccgaacga gatggcggtg ggaaccggcg ccaggccgat
     1801 gaagacgccg agcgaggtgg ccaccccctt gccacccttg aacgaagcga agggggtgag
     1861 gatgtggccg cagacggccg cgaggcctcc caccaggcgc aggtagggcc agctttccga
     1921 gcccggcagg agacccggca cgatggccgt ggcgatgtac cccttgccgg cgtcgagcag
     1981 gaggacgagc aatgcggccc aggcccccat cgtgcgatag acgttggtgg cgccgagatt
     2041 gccgctgccc gacttgcgga tgtctcccct gccgagcagg tgccccatcc agaggcccca
     2101 cggcatcgag ccgaggagga atccggtgag gatgaccgcc aggtagtagt tcatcgcctc
     2161 gaatctcccg aacccttggt gggacggcgc gccttgtagc gcacctcgat cggactccct
     2221 tcgaagccaa gcgactcgcg gagtgagttg atcacgtatt tgcgatagct ggccgcgaac
     2281 gccagcggtt cgttgacgaa gagggtgaag accggcgggg ccgtccccgt ctgggtgcca
     2341 taataaatcc tcggcggctt tccgacacgc ttgctggcgg gcggctggta tttctgctcc
     2401 atggccgtca ggaaacggtt gagttcggag gtggagatct cccgcttccg ctcccgggcc
     2461 agctgacggg ccagggccgg aattt
//
LOCUS       DASVOD010000350         5471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_76727, whole genome shotgun sequence.
ACCESSION   DASVOD010000350 DASVOD010000000
VERSION     DASVOD010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76727"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..2004)
                     /locus_tag="VF720_07965"
     CDS             complement(196..2004)
                     /locus_tag="VF720_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879330.1"
                     /translation="MMPRRHRVFMAIVVLVAALAGMWPGATEAALWTAVDTRLSSPGV
                     LALTSPGGRTLVRLPGNRMYAVWYERTGPKAAVVRGAERNGFGEWSMDPVVLSLGDSL
                     VRNPVLAAGPTGDLHLAWEDLRDGSEQIYYRHRTPLGAWEDEEAVTNDNGETLGPVLG
                     VDANGRLHLIWSDAISGNKELYYCQRDAGGAFTPPVRLTNHPGESIQPNFAIDSAGDI
                     QLVWQDGILDGGTDTNFNTEIWYMPLDSDGVPRAQPVRVSRALGFSQKPNLAIGADGA
                     IHFVWSDGRDSSPTNPNYFPQAIWYRRWLPGLGFGHEKRFVFSGADHLNPTVATTPDG
                     TVNIVWEDYILGNSELYYRQITPDTGWDVSPTRLTNTVGPTRGPSLLADGDGSLHLVW
                     SDAGSGEEGSIRYRSGQAGGTTPVRLADARIEAIDNTLRISWRTSEEIDHDAFLLYAA
                     DAEDEEARALPGLIRGGPDYTVTIARDSLAGAGLVKLVALDRFGALETVAVLLVPGQG
                     GIGTASLRAGPVHPNPVRDVLRLSYDLDVTTPVEAMLFDVGGRRVAGVALGMQDAGRH
                     ILNWEMATLARPAAGRYYLRITAGASVITRGVTLVR"
     gene            complement(2001..4802)
                     /locus_tag="VF720_07970"
     CDS             complement(2001..4802)
                     /locus_tag="VF720_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HEX7879331.1"
                     /translation="MLRSLFLTLVLLLGAETSRAVPVAPRDLEGVADPALRHLLTWEP
                     LGQDPPQALREAVASRMATGDDVTLRMLVVLADFMDRPASGDMLDIEAIRQRLFSVGE
                     LPHGSMADYYLENTQGRLLLTGEVIGWYRLPERYSTYVNGTAGLSFFPRNTQGMVRDA
                     ARLADADVNYASFDSDGPDGVADTGDDDKRVDLLLVMHSGGGNEGSSSPIELRSVAWF
                     LPDPAYVDGVNVSEFAIAPWSGGLGVVAHETGHLLGLPDLYDATGRSLGLGAWSLMSG
                     GWTLGETHTPAHLDAWCKTRLGVFDPIVVTSLREAEGIDPVETNGAVYKLLVPGAGGF
                     QYFLVENRRRIGFDEFLPGEGLLIYHVNELRPTNNIAPNYKVGLEQADGLFQLENLNG
                     NPSFGDEGDPWTASTPQEGFGRFTTPDTRNQEGQESGVAVYAVSGPDLSGRMTATLRP
                     SLGPVARLQTIEVTPLTGDGDRFLEPDETFAVTPSVLVSGGSVSDVVVSGTSSDPHLV
                     LETSAQPVGTLASGLQVLPSLVARVTGVLPTNPYGLPVSLRVGYREEVPTRSAITIGA
                     GDAEGMVADFTEGTDGFTNRVLRIDRLNVWRYETSAPNTGSRAWHAGNPTGGYPAGTD
                     CVLESPLFLIPPAGRLKLDHLVEIARDESMMVVAGGFVELSVNGGAWQSITPTGGYDA
                     FYFSGDPDLNRRGIFVGVRPAWAPVEFDLAQFHGSARVRFRFFSSAPRVGDRGWWIDN
                     LRVESSEVPVRLLDLAAAREADDVTLSWRLDPSDLPSALVVHRDRPGEAVNASGLVVL
                     PGNASGTWRDVGAPAGELVYRLEAIGRNGVTFPLGQVVVGGAPPLPLSLRAAPNPCRT
                     SARIAFSTPESGRATVRLFDSAGRMVRTLLDASLDPGEHSIDWDIRDDRGVTLPAGIY
                     FAKAATPSGSITKRLVVVP"
     gene            complement(4853..>5471)
                     /locus_tag="VF720_07975"
     CDS             complement(4853..>5471)
                     /locus_tag="VF720_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX7879332.1"
                     /translation="GTAAAEHAGRDPAVDGPAPVSALDRQLFDWINQGWANSFFDTLM
                     PIVSNSKSWGGAILMAAIALALLGGTVGRRTVLALVLSIALGDTLASQVLKPLFHRER
                     PCHALVEARVVGRCGGRNGFPSNHATNVGAAAAVLGTFYPASLVVALPAALLVGVSRV
                     YLGAHYPGDVLAGYLLGSAIGLLLARICRGIGRRPLPEGGASVTL"
BASE COUNT          949 a         1845 c         1878 g          799 t
ORIGIN      
        1 atccgtgact tcggtcgcta cccggaggac aggaaatgcc agattcgtgc cacggtcggg
       61 agccgctgaa acgtggactg attcgagatg gcgcccgcgt gccgggcagg ccggccctgc
      121 tccgggtgag gccacccgac gagtcggggc atctccccta tccatccgcg tgtgcggaga
      181 acctgccgcg caccgctatc ggaccagcgt gacgccccgg gtgatcaccg acgcaccggc
      241 cgtgatccgg aggtagtaac ggccggcggc cggccgggcc agcgtcgcca tctcccagtt
      301 caggatgtgc cgcccggcgt cctgcattcc gagcgcgacg cccgccacgc ggcggccgcc
      361 gacgtcgaac agcatcgcct ccacgggtgt ggtcacgtcg agatcgtacg aaagccggag
      421 cacgtcacgg accgggttgg gatgcacggg cccggctcgc aacgacgccg tgccgatccc
      481 tccctgcccg ggaacgagga gcaccgccac cgtctcaagg gcaccgaagc ggtcgagcgc
      541 caccagcttc acgaggccgg cgccggccag cgagtcgcgc gcgatcgtga ccgtgtagtc
      601 gggacctccc cggatgagcc cggggagtgc gcgcgcttcc tcgtcttccg catcggcggc
      661 gtagaggagg aaggcgtcgt ggtcgatctc ctcgctcgtc cgccacgaaa tgcgaagcgt
      721 gttgtcgatg gcctcgatcc gggcgtcggc caaccggacc ggggtggtgc cgccagcctg
      781 gccgcttcga tagcggatcg atccttcctc gcccgagccg gcatcggacc agacgaggtg
      841 gagcgagccg tcgccatcgg ccaggaggct cgggccgcgg gtggggccca ccgtgttggt
      901 gaggcgcgtc ggcgacacgt cccagccggt atccggcgtg atctgccggt aatagagctc
      961 gctgtttccg aggatgtaat cctcccagac gatgttgacc gtaccgtcgg gtgtggtggc
     1021 caccgtcggg ttgaggtgat cggcccccga aaagacgaat cgcttctcgt ggccgaaacc
     1081 gagaccggga agccaccggc gataccagat ggcctgcggg aagtagttcg gatttgtcgg
     1141 cgaagaatcc cggccatccg accacacgaa gtgaatggcg ccgtcggcgc cgatcgccag
     1201 gttcggcttc tgcgagaaac cgagtgcccg gctcacgcgc accggctgcg cccgcggcac
     1261 gccgtcgctg tcgagcggca tgtaccagat ttcggtgttg aagttggtgt cggtgccccc
     1321 gtccaggatc ccgtcctgcc agacgagctg gatgtcgccc gccgagtcga tcgcaaagtt
     1381 gggctggatc gattcgccgg gatggttggt cagccgcacg gggggcgtga aggcaccccc
     1441 cgcatcgcgc tggcagtaat aaagctcctt gtttcccgaa atggcatccg accagatgag
     1501 gtggagacgg ccgttcgcat cgacgccgag caccgggccc agcgtttccc cattgtcgtt
     1561 ggtcaccgcc tcctcgtcct cccaggcgcc gaggggcgtg cgatgccggt agtagatctg
     1621 ctccgaaccg tcgcgcaggt cttcccaggc gaggtgcagg tcgccggtgg gccccgcggc
     1681 caggacggga ttgcgcacca gcgagtcgcc cagcgagagg acgacgggat ccatcgacca
     1741 ctcgccgaag ccgttgcgtt cggcgccgcg cacgaccgcg gccttcggcc cggtccgctc
     1801 gtaccagacg gcgtacatgc gattgccggg aagccggacg agcgttcgac cgccaggcga
     1861 ggtgagcgcg agcacaccgg gagaggaaag ccgcgtgtcg acggccgtcc agagcgcggc
     1921 ttcagtcgct cctggccaca tgcccgccag ggcagccacg aggacgacga tcgccatgaa
     1981 gacgcgatga cgccgtggca tcatggcacc accaccaggc gtttggtgat cgaaccggac
     2041 ggcgtggcgg ccttcgcgaa gtagattccg gccggaagcg tgacgccccg atcgtcccgg
     2101 atgtcccagt cgatcgaatg ctcacccgga tcgagcgatg catcgaggag cgtgcgcacc
     2161 atgcgcccgg ccgagtcgaa aagccggacc gtggcgcgac ccgactccgg cgtcgagaag
     2221 gcgatccgtg cgctcgtccg gcagggattc ggggccgctc ggagcgacag ggggagcggc
     2281 ggagccccgc cgacgaccac ctgtcccagc ggaaacgtca cgccatttcg accgatagcc
     2341 tccagccggt agacgagctc accggctggc gccccgacgt cgcgccaggt tccgctggca
     2401 ttgccgggca gcaccacgag gcccgaagcg ttgaccgcct cgccgggacg atcgcgatgg
     2461 accacgagcg ccgaagggag gtcggaagga tcgagacgcc acgagagcgt gacatcgtcg
     2521 gcctcacgcg ccgccgcgag atcgagcagc cggaccggca cctcggacga ctcaacacgg
     2581 agattatcga tccaccatcc acgatcccca acccgcgggg ccgatgaaaa gaaacggaag
     2641 cggacgcggg cgctgccgtg gaactgggcc aggtcgaact ccaccggcgc ccatgccggg
     2701 cgcaccccca cgaagatccc gcgccggttc aggtcggggt cgcccgaaaa gtagaaggca
     2761 tcgtagccgc cggtcggcgt gatggactgc cacgcgccgc cgttcaccga aagctccacg
     2821 aagccgccgg ccacgaccat catcgattcg tcacgggcga tctcgacgag atgatcgagc
     2881 ttgagccggc ccgccggcgg gatcaggaag aggggcgatt ccagcacgca gtcggtgccc
     2941 gccggatagc ccccggtggg attgccggcg tgccatgccc ggctccccgt gttcggcgca
     3001 ctcgtttcgt accgccagac gttcagccgg tcgatccgga ggacgcgatt ggtgaaaccg
     3061 tcggtccctt ccgtgaaatc ggccaccatt ccctcggcgt ccccggcgcc gatggtgatg
     3121 gcggaacggg tcggcacctc ttcgcgatac cccacacgga ggctgaccgg cagtccgtag
     3181 ggattggtgg ggaggacgcc ggtcacccgg gccaccaggg acgggagtac ctgaagcccg
     3241 ctcgccagcg tgccgaccgg ttgcgctgac gtttccagca cgagatgggg atccgaactc
     3301 gtgccggaga cgaccacgtc ggaaacgctt ccgccggaca cgagcaccga cggcgtgacg
     3361 gcgaacgtct cgtcgggttc gaggaaacgg tcgccgtcgc cggtgagagg cgtgacctcg
     3421 atcgtctgga gccgggccac cggcccgagg gacgggcgga gcgtggccgt catgcgcccc
     3481 gagagatccg gacccgatac ggcgtacacg gccacgccgc tctcctgccc ctcctggttc
     3541 ctcgtgtcgg gcgtcgtgaa tcgaccgaat ccttcctggg gcgtggacgc ggtccaggga
     3601 tccccctcgt cgccgaagct cggattgccg ttcaggtttt ccagctggaa gagaccgtcg
     3661 gcctgctcga ggcccacctt gtagttggga gcgatgttgt tggtcggccg cagttcattg
     3721 acgtggtaga tgagcaaccc ctcgccgggg agaaactcat cgaacccgat ccgccggcga
     3781 ttctcgacca ggaagtactg gaaccccccg gccccgggga ccaggagctt gtagaccgcg
     3841 ccgttcgttt cgaccgggtc gatcccctcg gcttcgcgca gcgaggtgac gacgatcgga
     3901 tcgaagactc cgagccgcgt cttgcaccag gcatcgaggt gcgccggcgt gtgcgtttcc
     3961 cccagggtcc agccgcccga catgagtgac caggccccga ggccgagcga acggccggtg
     4021 gcatcgtaga gatcgggcag gccgaggagg tggcccgttt cgtgggccac gacgccaagc
     4081 ccgccgctcc agggggcgat cgcgaactcg ctcacgttga cgccgtcgac atacgccggg
     4141 tccggaagga accacgccac gctccggagt tcgatgggtg acgacgaccc ttcattgcct
     4201 ccgcccgagt gcatcaccag gaggagatcg acgcgcttgt cgtcgtcgcc ggtgtcggcc
     4261 acgccgtcgg gaccatcact gtcgaagctg gcgtagttga cgtcggcgtc ggccaggcgg
     4321 gcggcgtcgc gcaccatccc ctgcgtgttg cgcggaaaga aggagaggcc ggcggttccg
     4381 ttcacgtagg tggagtagcg ttccggaagc cggtaccagc cgatcacttc gcccgtcagg
     4441 aggagccgcc cttgcgtatt ctcgaggtaa tagtcggcca tgctgccgtg cggcaactcg
     4501 cccacgctga agagacgctg ccgtatggcc tcgatgtcga gcatgtcgcc cgaagcgggg
     4561 cggtccatga aatcggccag caccaccagc atccggagcg tgacatcgtc cccggtggcc
     4621 atgcgcgagg ctactgcttc gcggagcgcc tgtggcggat cctggccaag cggctcccag
     4681 gtcaggagat gccggagagc cgggtcggcg acgccctcga gatcgcgggg ggctacagga
     4741 acagcccggg acgtttcagc cccgagcagg aggacgagcg tgaggaacag cgaacgaagc
     4801 atcggacctt catggccggc ggcagctgcc ggcaccgaga acaaccccgt gctcacaagg
     4861 taacggacgc cccgccttct ggcaacggtc gacggccgat tcctcgacaa atacgtgcca
     4921 ggaggagtcc gattgccgac ccgagcaggt agccggccag cacgtccccc ggatagtgcg
     4981 cccccaggta gacgcgcgag acccccacga gcagcgcggc ggggagtgcc acgaccaggc
     5041 tcgccggata gaacgtaccc aggaccgccg ccgcggcccc gacattggtg gcgtgattcg
     5101 aaggaaatcc attgcgacca ccacaacggc cgacgacgcg tgcctcgacc agcgcgtgac
     5161 acgggcgctc gcggtggaag aggggcttca gcacctgcga cgccagcgtg tctcccaggg
     5221 cgatcgacag gaccagggcc aggaccgttc gacgtccgac agtgccacca agcagggcca
     5281 gggcgatcgc agccatgagg atcgcgcctc cccagctctt cgaattgctg acgatcggca
     5341 tcagcgtgtc gaagaatgaa ttcgcccacc cctggttgat ccagtcgaag agttgccggt
     5401 cgagcgcgct cacgggtgcc ggtccatcga cggcgggatc gcggccagcg tgttcagctg
     5461 ccgccgtgcc g
//
LOCUS       DASVOD010000351         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_285424, whole genome shotgun sequence.
ACCESSION   DASVOD010000351 DASVOD010000000
VERSION     DASVOD010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285424"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /gene="nadE"
                     /locus_tag="VF720_07980"
     CDS             <1..630
                     /gene="nadE"
                     /locus_tag="VF720_07980"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX7879333.1"
                     /translation="ISLKTIPIDGIFGKYLDDLAAGLEGLPANATEENIQARIRGNVL
                     MALSNRFGHLVLSTGNKSEIAVGYCTLYGDMCGGLCVISDLYKTEVYRLAEHLNRHEE
                     RIPVSTITRPPSAELRANQKDQDTLPPYEMLDGILRLYVDENLDEDGIVDHGYDRPIV
                     RDVIRRLEINEYKRQQAAPGLRVSWKAFGMGRRYPIARKIPMAAGGIAS"
     gene            627..>1785
                     /locus_tag="VF720_07985"
     CDS             627..>1785
                     /locus_tag="VF720_07985"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7879334.1"
                     /translation="MIRRTLLAMVTLALFGGANAEAAKVSQVYISEVLYNPVGVDGGN
                     QLIELVNRGSTAQSLNPWSICIQFLYKNFPVGASIPAGGRYTIHINTIGSNTATDWYT
                     GTGYVNIDVTGDAISLYHTTSGFTTSGNMEDFVQFGAGGQPRENVGVAAGFWTAGTFV
                     PLGAEGQSVQFVPAATPPATRHPITDYCNAPPTIGVANACGPPPMGTLDDLRITEVLA
                     DPMGPEAGATRAELINTSALPTSLDGLTFRIGGSHFTVPNGLTAPAGGLILVNLNSAG
                     APGWLAPLSDARPGFEADVTINAVPFVDLTARAGSFSLYRNALDFASPANMVDFLAWG
                     AAGQGDDSVAVARGIWPAGAYFPEVAEGSSIQWRGTNNGNSPDDWCSDVPTL"
BASE COUNT          324 a          617 c          569 g          275 t
ORIGIN      
        1 atttcgctca agacgattcc catcgacggg atcttcggga agtacctcga cgacctggcg
       61 gcgggcctcg aggggctccc cgccaatgcc accgaagaga acatccaggc ccgcatccgc
      121 ggcaacgtcc tgatggccct ctccaaccgt ttcggtcacc ttgtcctctc gaccggcaac
      181 aagtccgaga tcgccgtcgg gtactgcacg ctgtacggcg acatgtgcgg cggcctctgc
      241 gtgatttccg atctgtacaa gaccgaggtg taccggctgg ccgaacacct caatcgccac
      301 gaggaacgca tcccggtttc cacgatcacg cgccccccgt cggcggaact ccgcgcgaat
      361 caaaaggacc aggacacgct ccccccctac gaaatgctgg acggcatcct gcgcctctac
      421 gtggacgaaa acctggacga ggacggcatc gtcgaccatg gctacgaccg gccgatcgtc
      481 cgggacgtga tccggcgtct ggaaatcaac gaatacaagc gacagcaggc ggcgcccgga
      541 ctgcgggtgt cgtggaaggc attcgggatg gggcgtcgtt atcccatcgc gcggaaaatt
      601 cccatggcag caggaggcat cgcatcatga tccggagaac gctgctggcg atggtgacac
      661 tcgcgctctt cggcggcgcg aacgccgagg cggccaaggt gtcgcaggtt tatatcagcg
      721 aggtgctcta caaccccgtg ggagtggacg gcggcaacca gctgatcgag ctggtgaacc
      781 ggggaagcac ggcgcagagc ctgaatccgt ggagcatctg catccagttc ctctacaaga
      841 attttccggt cggcgcatcg atacccgccg gcgggcgtta cacgatccac atcaacacca
      901 tcggttcgaa caccgccacc gactggtaca cggggaccgg ctacgtgaac atcgacgtga
      961 ccggcgacgc gatctccctc tatcacacca ccagcggctt cacgacgagc ggcaacatgg
     1021 aggatttcgt gcagttcggg gccggaggcc agccgcgcga gaacgtgggc gtggccgccg
     1081 gtttctggac cgcaggaacc ttcgtgcctc tgggcgccga ggggcagagc gtgcagttcg
     1141 tgccggccgc cacgccgccg gccacccgcc acccgatcac ggactactgc aacgccccgc
     1201 ccaccatcgg cgtcgccaac gcctgcggtc cgccgccgat ggggacgctg gacgacctgc
     1261 gcatcaccga ggtcctggcg gacccgatgg gccccgaagc cggcgccacc cgcgccgaac
     1321 tgatcaacac ttcggcactg ccgaccagtc tggacggact cactttccgg atcggcggca
     1381 gtcacttcac cgtgccgaat ggcctcaccg cgccggcggg ggggctcatc ctggtcaacc
     1441 tgaacagtgc cggcgcgccc ggatggctcg cgccgttgtc cgatgcacgc cccgggttcg
     1501 aagccgacgt gacgatcaac gccgtgccgt tcgtcgacct gaccgcacgg gccggttcct
     1561 tttcgctgta ccggaacgcg ctcgacttcg ccagcccggc caacatggtc gacttcctcg
     1621 cgtggggcgc cgccggccag ggggacgact cggtggccgt ggcccgggga atctggccgg
     1681 cgggggccta cttccccgag gtggccgagg ggtcgagcat ccagtggcgc ggcaccaaca
     1741 acggcaactc gccggacgac tggtgctccg acgtgccgac gctgg
//
LOCUS       DASVOD010000352        20202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_649412, whole genome shotgun sequence.
ACCESSION   DASVOD010000352 DASVOD010000000
VERSION     DASVOD010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 20202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20202
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649412"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1458
                     /locus_tag="VF720_07990"
     CDS             7..1458
                     /locus_tag="VF720_07990"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX7879335.1"
                     /translation="MSGSKSFHSKQFQQLFVDMREGKVNTVLCTALDRVSRSVKDLMA
                     FAEILDELKVEFVCLKQNYDTTTPHGRFFFTMMAALSELERGQTSDRNKESYRARAER
                     GLWNGGQLLGYDLPPDGQKGTLIPNEREAAIVNFAFDTYLKTGSILETAKALNEVGYR
                     TKEYESRRGRLHAASKFSWTSTMWLLTNWGYIAKKEINKKKKGVSLARTSTEVEYGQV
                     DAKWPPIVDTAKFYAVRDLMEANSRSCRNQMKKVRHTYILNHGLLNCGKCNTEMEGTC
                     GTGRLKTKHFYYVCKNRECRLRIPAGEIEGLILERVKELALSPDILGRLVTETNQRLQ
                     TELPDLTEKRAATDRDLKAVTAEAKKLLARWQDTPEADSFVRAQLADLEERRKALELA
                     VVDLDQRLAALGRTTVDEGVVRKALAAFAEGFAGLEPYRQKAMIGLVIQQATVSDDSL
                     TLSIHGEPVVIGEKETSGNAVNARPDSGAWLLR"
     gene            complement(1444..1518)
                     /locus_tag="VF720_07995"
     tRNA            complement(1444..1518)
                     /locus_tag="VF720_07995"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1484..1486),aa:Asn,seq:gtt)
     gene            complement(1613..2875)
                     /locus_tag="VF720_08000"
     CDS             complement(1613..2875)
                     /locus_tag="VF720_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HEX7879336.1"
                     /translation="MTRVTHLNARMPRLLTGALAILLLASLPISAQASHRPALTDGRA
                     AWVKRDQLMSPARIQRTVDDAANAGLNVLFVQVRGLGDAWYNSDIVPKATQIEGMAFD
                     DGTPMDPLDLLIRRAHAKGIEVHAWLNVFMVWQGDSDPKLTDHVIKRHPDWVAVDARG
                     RPMTSYTTTELQQARTEGVFLASGNPQVRKHLCAMALEVAERYDVDGIHLDYIRNPLL
                     ETGFDKVTRAGFLAEKGVDPWKLRNAAIPLKARYGAEGLAQLEKDWKEWRADQVTRLV
                     RDIHDGLEALDKPVTLSAAVFPNCNYAPNDVGQAWMTWCQEGLLDLVVPMMYSPKSST
                     VMDQFMLAQKTMPLNVVMYAGLAVYNQPLTSVVESARRLNDAGIDGICFFPYDTLAEK
                     PGTLERLSRACFGGTEAPARRVKTATAR"
     gene            complement(2872..3609)
                     /locus_tag="VF720_08005"
     CDS             complement(2872..3609)
                     /locus_tag="VF720_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7879337.1"
                     /translation="MPLHIQNLTKTYANGTQALKDITLTIPTGLTVIAGPTGAGKSTL
                     LRILATLQEPDRGSVHLNGIDILNRREAACRSIAWLSHDPGVHPSLSPWERLDHVALR
                     SGIIDDRLRKATVEQLLRKTDLWEQRNRPMTDDAHAHTLATQRRLALAVTLIGSPALI
                     LVDEPATALDPTERTPFLELLTELAEDRIVLIAATTTHSLTGPRPRMAIMDRGELRQI
                     VEPPSFTSAPTALFPYPSHRSVPIIRA"
     gene            complement(3839..4456)
                     /gene="rplQ"
                     /locus_tag="VF720_08010"
     CDS             complement(3839..4456)
                     /gene="rplQ"
                     /locus_tag="VF720_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX7879338.1"
                     /translation="MRHRRDHRHLSRTSEHRRALLRNLVTELFRHERIETTVAKAKEA
                     RRVAERMITFAKRGDLHARRQVASYLFDETVLTKLFDTIAPWYTTRQGGYTRILKTRR
                     RLGDAGEMGIFELVKSKEQLEKERQDRLAAEAAAREAATKKGGKKKAAAEDEAEVEEA
                     PRKAKGRGPKGTKEARTKSMPGTRGKTGAAQKAQKKGASKASKKG"
     gene            complement(4477..5676)
                     /locus_tag="VF720_08015"
     CDS             complement(4477..5676)
                     /locus_tag="VF720_08015"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX7879339.1"
                     /translation="MKWKTLQMPRGIEIDSSVSNDRYGKFTIEPLERGFGMTIGNSLR
                     RVLLSSLQGAAISSVKIDGVLHEFSTIPGVLEDVTEIILNLKQTRYKLHADYQSRARF
                     KVAGPAEVLAGDLEHGPEVEVLNPDQHICTVNKEGTLHMEVEITAGRGYLSGEGAVEE
                     DRPIGEIPVDAHFSPIKKVNFFVENTRIGQRVDYDKLTVEVWTDGSILPQDAVAYSAK
                     ILQDHFALFVQFQEPIETDQDEVVDADMARIRDLLLKNVEELELSVRSANCLRAANIR
                     SIGELVRKPESEMLKYRNFGRKSLKEISDILTEMGLGFGMDVTPYLGPQAPLPPPPAD
                     MLVGGDDDDDYDYEDDDDDDEDDDLEDDTIDASSDADDDEDEKTPVGPAASDDDDAEP
                     ADDDDKN"
     gene            complement(5759..6391)
                     /gene="rpsD"
                     /locus_tag="VF720_08020"
     CDS             complement(5759..6391)
                     /gene="rpsD"
                     /locus_tag="VF720_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX7879340.1"
                     /translation="MARYRDAVCRLCRREGMKLFLKGERCFTDKCAIERRQYAPGDHG
                     RDRRTKLTNYGVQLREKQKARRIYGIMEKQFRNYFATAAGSSGVTGETLLQLLETRLD
                     SIFFRLGFAASRSQSRQLVRHRHVTVNGRIVDIPSYRVKPGDVVAVREKSRQNVVIQA
                     ALEGVGRRGETPDWIEVNEKTFSGRITRVPSRDGIPTQVQENLIVELYSK"
     gene            complement(6408..6797)
                     /gene="rpsK"
                     /locus_tag="VF720_08025"
     CDS             complement(6408..6797)
                     /gene="rpsK"
                     /locus_tag="VF720_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX7879341.1"
                     /translation="MAKPKEKRKKKDKRVGISGVAHIKATFNNTIVSITDGEGNVISW
                     ASAGKVQFKGSRKSTPFAAQVAAENAAREALGLGLRKVEVLVKGPGAGREAAVRSLQA
                     AGLEIQSIRDVTPIPHNGCRPPKKRRV"
     gene            complement(6810..7187)
                     /gene="rpsM"
                     /locus_tag="VF720_08030"
     CDS             complement(6810..7187)
                     /gene="rpsM"
                     /locus_tag="VF720_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX7879342.1"
                     /translation="MARIAGVDLPMGKRVEVALTYIYGIGPNLSRNIVRATHVNPDTR
                     VKDLSEEETGRLRTEIETRYRVEGQLHTEVEMNIKRLKDIGSYRGLRHRKGLPTRGQR
                     TRTNARTRKGPKKTMAGKKKPTK"
     gene            complement(7200..7313)
                     /gene="rpmJ"
                     /locus_tag="VF720_08035"
     CDS             complement(7200..7313)
                     /gene="rpmJ"
                     /locus_tag="VF720_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX7879343.1"
                     /translation="MKVKASVKKICEHCKLVRRKGVVRVICKNPRHKQRQG"
     gene            complement(7338..7556)
                     /gene="infA"
                     /locus_tag="VF720_08040"
     CDS             complement(7338..7556)
                     /gene="infA"
                     /locus_tag="VF720_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014488934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX7879344.1"
                     /translation="MSKQEGVQVEGTVIEPLPNAMFRVELENKHVVLAHISGKMRMNF
                     IRILPGDKVTVELSPYDLSRGRIVYRYK"
     gene            complement(7610..8356)
                     /gene="map"
                     /locus_tag="VF720_08045"
     CDS             complement(7610..8356)
                     /gene="map"
                     /locus_tag="VF720_08045"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX7879345.1"
                     /translation="MIRIKSPSEVEAMRGSARLVSRTLDELAGHVKAGVTTLELDRLA
                     MEFIRKNGGTPSFLGYRGYRHTICASVNEEVVHGIPGKRVLKDGDIISVDVGVLMNGF
                     HGDSARTFTVGAVTGQAEQLLKVTRESLLKGIEQVRAGNRLGDVSSAIQAHVESHGLS
                     IVRSLVGHGIGRDLHEDPQIPNYGQPGSGVVLKPGMVFAIEPMVNVGGWEVETLKDGW
                     TVVSSDRSLAAHFEHTVAVTENGPDILSLS"
     gene            complement(8376..9023)
                     /locus_tag="VF720_08050"
     CDS             complement(8376..9023)
                     /locus_tag="VF720_08050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017603900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX7879346.1"
                     /translation="MIFILLGPPGVGKGTQARLLADRFGVQSVATGDMIRAEVANDTP
                     LGREARSFMDAGDLVPDDVILRMVGERMKNGKGGGFLLDGFPRTLAQAEGLDRILTKI
                     GKPLIGVVSLAVPEENVVERLGNRRTCPVCGRVYNLASQPPRMPGRCDDHPEAELILR
                     TDDAPDTVRHRFSVYNAQTRPLVDWYRSRGLLREVNGIGPVEEVLGRVEAALRSS"
     gene            complement(9041..10393)
                     /gene="secY"
                     /locus_tag="VF720_08055"
     CDS             complement(9041..10393)
                     /gene="secY"
                     /locus_tag="VF720_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX7879347.1"
                     /translation="MFDRIGEFFTSFRNALSIPELRKRLIFTGLVLVVYRMGSHIPIP
                     GINGDALSALVRQAGNGVFGLYDMFAGGNLSKATIFALGIMPYISASIILQLFGAVIP
                     YFEKLQKEGEEGRKKINQYTRWGTVFLAAIEAAGASFFLQSSGFTGPDGQPIVSHSGP
                     GFVMMTVITLTAGTMFVMWLGEQITERGIGNGISLIIFIGIVARYPLDIMNTLRSEAA
                     TGWNFLKFVKLGFILALMLAVIMATILITQGQRKIPVQYAKRMVGRKMYGGQNTHIPL
                     RVNTAGVMPIIFAQALVSIPGTIASFFRTNEFASSIAQIFAPHHAVYLILYSLAIIFF
                     TYFYTAIVLNPVDLADNMRKHGGFVPGIRPGKKTAEYIDRILTRITVPGAIFLAFIAV
                     LPDVMINLLRVPFYFGGTGLLIVVGVALDTLAQIESHMLMRHYDGFMKKGRLKARFGR
                     "
     gene            complement(10396..10881)
                     /gene="rplO"
                     /locus_tag="VF720_08060"
     CDS             complement(10396..10881)
                     /gene="rplO"
                     /locus_tag="VF720_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX7879348.1"
                     /translation="MTITTVKPARGAVKKKKRLGIGTGSGHGGTSTRGHKGVKARSGP
                     NIPAWFEGGQMPLQRRLPKVGFTNIFRVEYHTINVGDLNDVTIAGTIDLAGLKKAGRL
                     SGGQKLLKILGDGELSKALTIKADAFTRGAIAKIQAAGGTVQVIEKPKRPKRFPKKAK
                     A"
     gene            complement(10890..11075)
                     /gene="rpmD"
                     /locus_tag="VF720_08065"
     CDS             complement(10890..11075)
                     /gene="rpmD"
                     /locus_tag="VF720_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX7879349.1"
                     /translation="MAKEIHVTQVRSIIKSGFKMERTIRALGLTRMHQTVVHRDTPQI
                     RGMIDKVKHLISVKEVG"
     gene            complement(11068..11607)
                     /gene="rpsE"
                     /locus_tag="VF720_08070"
     CDS             complement(11068..11607)
                     /gene="rpsE"
                     /locus_tag="VF720_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX7879350.1"
                     /translation="MTPRYESQESEYVEHVVQINRCAKVVKGGRRFSFNAIVTVGDKM
                     GRVGIGLGKANEVADAIRKASEAARRHMVLIPLVKGTIPHEIIGEFGAGRVLLKPASP
                     GTGVIAGGAVRPVLEACGVQDVLTKSIGSTNPHNAVKAVFNGLTRLRKIGDVARMRRK
                     HVSELFAPKAEAAAEASNG"
     gene            complement(11621..12004)
                     /gene="rplR"
                     /locus_tag="VF720_08075"
     CDS             complement(11621..12004)
                     /gene="rplR"
                     /locus_tag="VF720_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX7879351.1"
                     /translation="MKIISRDRRTAREKRHRRIRRKMSGEASCPRLCVFRSDKHIYGQ
                     LVDDVTGTTLLTVGSLSVDVKSRIGSLEGASGKSGRAKAVGIVLAEKAKEKGVTRVRF
                     DRGGYLYHGRVKALADGAREGGLEF"
     gene            complement(12020..12559)
                     /gene="rplF"
                     /locus_tag="VF720_08080"
     CDS             complement(12020..12559)
                     /gene="rplF"
                     /locus_tag="VF720_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX7879352.1"
                     /translation="MSRVGKKIINLPSGVSVDWKDGEVRVKGPKGELSYRPLAGVDLK
                     MEGNTLQIVNTSEERTGPAIHGLTRALINNQVVGVSEGYRKVLEIVGTGYRAALAGRS
                     LTLQLGFSHSIEFPLPAGIEAKVESPTRLEISGIDKQLVGQVAANIRRLRPPEPYKGK
                     GVKYEGEYIRRKAGKSAGA"
     gene            complement(12573..12971)
                     /gene="rpsH"
                     /locus_tag="VF720_08085"
     CDS             complement(12573..12971)
                     /gene="rpsH"
                     /locus_tag="VF720_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX7879353.1"
                     /translation="MSVSDPIADMLTCIRNAAQARHRRVDVPASRIKQEIAGVLLREN
                     YVANVKRMEDGKQGMLRIYLRYDDEEKSIITGIKRVSSPGRRIYVNRHTVPRVQGGMG
                     TAIVSTSQGILTDREARVRGLGGELLCMIW"
     gene            complement(13067..13252)
                     /locus_tag="VF720_08090"
     CDS             complement(13067..13252)
                     /locus_tag="VF720_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX7879354.1"
                     /translation="MAKKSMIERWKSTPKFKVRHHNRCTRCGRPRAFLRKFALCRICF
                     RDLALFGQIPGVTKASW"
     gene            complement(13293..13841)
                     /gene="rplE"
                     /locus_tag="VF720_08095"
     CDS             complement(13293..13841)
                     /gene="rplE"
                     /locus_tag="VF720_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX7879355.1"
                     /translation="MSTPRLRKDYEERVVPAIMERFQLKNRHQAPKLTKIVVNMGVGD
                     AIQNAKLMDAAMNDLTAITGQRPSVRRAKKSISNFKLRENLAIGCAVTLRGARMWEFL
                     DRLVSVAIPRIRDFRGLPQRSFDGQGNYSFGIEEQIIFPEINYDKVEKVRGMDVTFVT
                     TAPDDEQGMELLKLMRFPFRER"
     gene            complement(13861..14193)
                     /gene="rplX"
                     /locus_tag="VF720_08100"
     CDS             complement(13861..14193)
                     /gene="rplX"
                     /locus_tag="VF720_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX7879356.1"
                     /translation="MGRMRIAKGDTVIVIAGDDKGKTGKVLMVMPEKNRVIVEKVNFV
                     KRHIKPTQRNTQGGIQEKEAPIHLSNVQLVDPKTGEPTRIGVKRSPEGGRERIARKSG
                     ETLPRPGR"
     gene            complement(14196..14564)
                     /gene="rplN"
                     /locus_tag="VF720_08105"
     CDS             complement(14196..14564)
                     /gene="rplN"
                     /locus_tag="VF720_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX7879357.1"
                     /translation="MIQMRSILKVSDNSGARVARMINVLGGSHKRYARIGDIVVASIQ
                     DAIPGGTVKKGDVVKGVVIRTRKEHRRKDGTYIRFDQNAIVIIDDAREPRGTRIFGPV
                     ARELRDKQFMKIISLAPEVV"
     gene            complement(14577..14861)
                     /gene="rpsQ"
                     /locus_tag="VF720_08110"
     CDS             complement(14577..14861)
                     /gene="rpsQ"
                     /locus_tag="VF720_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018943599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX7879358.1"
                     /translation="MTASDTTEAGEERGQRKMRVGKVMSSKMDKSAVVVIERLVKHPE
                     YGRYIRRRKKFVIHDEKNDCQEGDTVRFMETRPLSRTKRWRLVEVVERAK"
     gene            complement(14881..15102)
                     /gene="rpmC"
                     /locus_tag="VF720_08115"
     CDS             complement(14881..15102)
                     /gene="rpmC"
                     /locus_tag="VF720_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX7879359.1"
                     /translation="MPRDLNVEKVRSMEATDIETRIKGLEEELFNLRFRNAMKQLENP
                     LEIRNLRRDLARLKTILAEHRSGSRPAGA"
     gene            complement(15104..15517)
                     /gene="rplP"
                     /locus_tag="VF720_08120"
     CDS             complement(15104..15517)
                     /gene="rplP"
                     /locus_tag="VF720_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX7879360.1"
                     /translation="MLLPSRVKHRKQQRGRMRGKAHRGGTIAFGDFGLQALEPAWVTN
                     RQIEAARVAITRHIKRGGKMWIRVFPDKPITVKPAETRMGKGKGNPEFWVAVVKPGRV
                     MFELQGIESALAQQALKLGAAKLPIKTKFIVREEF"
     gene            complement(15530..16342)
                     /gene="rpsC"
                     /locus_tag="VF720_08125"
     CDS             complement(15530..16342)
                     /gene="rpsC"
                     /locus_tag="VF720_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX7879361.1"
                     /translation="MGQKTNPIAMRLGIIRTWDSRWFARKKEYATSLGEDVKVRKYIR
                     QRLAQAGVSKIIIQRASNKMTINIATAKPGLVIGRRGAQVDQLRDELHHITGKEIYLN
                     IDEIKKPDLDAQLVAMHIARQLEGRVSFRRAMKKAIQASMKSGALGVRVACSGRLGGS
                     EMARFETYREGRVPLHTLRADIDYAIATAYTTFGTCGVKVWIFHGEVLENRPAELGEA
                     QAERPRGGREAGREGAGRDSGRDGGRGRDGRDNRDGGRGGRDRDQAPAGGAR"
     gene            complement(16354..16896)
                     /gene="rplV"
                     /locus_tag="VF720_08130"
     CDS             complement(16354..16896)
                     /gene="rplV"
                     /locus_tag="VF720_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX7879362.1"
                     /translation="MEKQTMEAKAIARYVRGSTRKTNQVLDLVRGKSVDNAMQTLQFS
                     TKHVARTVLKVLKSAVANATVQEARLHTEDLFVKECLCGPAPIMKRFLPRAQGRATPL
                     LKRSCHITIIVGTKARKGAAVAAPTPATTAAPKAAPKTAAKSGATKAAASKAAPKKKT
                     AAKKAVKAKSPARAAAAGKK"
     gene            complement(16908..17207)
                     /gene="rpsS"
                     /locus_tag="VF720_08135"
     CDS             complement(16908..17207)
                     /gene="rpsS"
                     /locus_tag="VF720_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX7879363.1"
                     /translation="MARSLKKGPFIDPKLMLKIEAMNRRGEKKVLKTWARRSTISPDF
                     VGHTLAVHNGTKFIPIYVTENMVGHKLGEFAGTRTFKSHSASGDKAAQKPGPPKK"
     gene            complement(17222..18046)
                     /gene="rplB"
                     /locus_tag="VF720_08140"
     CDS             complement(17222..18046)
                     /gene="rplB"
                     /locus_tag="VF720_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX7879364.1"
                     /translation="MGIKKFRPLTPSLRYKTVSDFADITRTEPEKSLLEPLRSTGGRD
                     NKGHTSTWYRGGGHKRRYRIVDFRRDKRGIPGRVDSIEYDPNRTARIALLVYADGEKR
                     YIVAPDGLKVGDRLEAGPEVEIRVGNALPLRAIPLGTNIHNLELIPGRGGQVARGAGA
                     ACQLVAKEGTHAQVKLPSGEVRIFHQECYGTIGQVGNLDHENIVIGKAGRVRWAGRRP
                     HNRGVSMNPVDHPMGGGEGRTSGGRHPCSPWGLPTKGYKTRKTKPSDRLIVRRRKK"
     gene            complement(18098..18388)
                     /locus_tag="VF720_08145"
     CDS             complement(18098..18388)
                     /locus_tag="VF720_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX7879365.1"
                     /translation="MKSAHNMVVRALITEKGTVMREKSNKYVFQVDAGANKIEIRKAV
                     ESIFNVHVVEVRTMNVPGKQKRMGKFAGYRPDWKKAIVTVKAGEAIELFEQI"
     gene            complement(18385..19011)
                     /gene="rplD"
                     /locus_tag="VF720_08150"
     CDS             complement(18385..19011)
                     /gene="rplD"
                     /locus_tag="VF720_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX7879366.1"
                     /translation="MATAKHFSAAGQEKGTVELPEAAFGAPLRPDVVYDALRSYRANQ
                     RQGTVATKTRSLVSGGGKKPWRQKGTGRARHGSTRSPIWVGGGTVFGPQPRDYSYQIP
                     KKMRRLAIRSILSERAKDGLVYVVDDLTLDAPKTKTIAGLFKTLALSDKRVLYVGKGP
                     DITLYRSVRNIAGARCIPADQISVYDVLATDAIVIQNGALEVLKGVAE"
     gene            complement(19011..19649)
                     /gene="rplC"
                     /locus_tag="VF720_08155"
     CDS             complement(19011..19649)
                     /gene="rplC"
                     /locus_tag="VF720_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX7879367.1"
                     /translation="MSAIIGRKIGMTQLYSEDGRVVPVTVLEAGPCPVVGKRTADKHG
                     YDALQIAYGDIREKVMSKPRLGQFKKAGQAPKRLLREVPVMEGLEVGGNVTVDRFAVG
                     AKVDVTGISKGRGFAGVVRRHHFSGGDNAHGCKTKKQPGSIGASAYPSRVIKGKRLPG
                     HMGAKQITVKNLKVVGIDLDENLIWVRGAVPGHTNAWVMIKSAPGDAVKGGK"
     gene            complement(19756..20064)
                     /gene="rpsJ"
                     /locus_tag="VF720_08160"
     CDS             complement(19756..20064)
                     /gene="rpsJ"
                     /locus_tag="VF720_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX7879368.1"
                     /translation="MSAQRIRIRLRSYDHAILDLSAGEIVKTARRTGAKTVGPVPLPT
                     RKSVFTVNRSPHVDKKSREQFEMRIHKRLIDISESTAQTIDALMKLDLPAGVDIDIKL
                     "
     gene            complement(20080..>20202)
                     /gene="tuf"
                     /locus_tag="VF720_08165"
     CDS             complement(20080..>20202)
                     /gene="tuf"
                     /locus_tag="VF720_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819280.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX7879369.1"
                     /translation="DNVKMSIELITPIAMDEGLRFAIREGGRTVGAGVVSKILE"
BASE COUNT         3106 a         6420 c         6693 g         3983 t
ORIGIN      
        1 cctggaattt ctggctctaa atcctttcac tccaagcagt ttcagcagct attcgttgac
       61 atgcgggagg gaaaggtgaa cacggtcctg tgcacggcgc tggaccgggt aagccgatcg
      121 gtcaaggacc tgatggcctt cgccgagatc ctggacgagc tcaaggtcga attcgtctgc
      181 ctcaaacaga actacgacac gacgacgccg catggtcggt tcttcttcac catgatggcg
      241 gccctgtctg aactcgaacg agggcagacg tccgaccgaa acaaggagtc ctaccgcgcc
      301 cgggccgaac ggggcctctg gaacggcggc cagctcctcg gctacgacct gccgccggat
      361 ggccaaaagg gcaccctgat cccgaacgag cgcgaggccg cgatcgtgaa cttcgccttc
      421 gacacctacc tcaagacggg gtcgatcctc gaaaccgcca aggcgctgaa cgaggtcggg
      481 taccggacca aggagtacga gtcccggcgc gggcgccttc acgccgcctc caagttctcc
      541 tggaccagca cgatgtggct tctgaccaac tggggataca tcgccaagaa ggaaatcaac
      601 aagaagaaga aaggcgttag cctcgcccgg accagcacgg aggtcgaata cggccaggtg
      661 gacgccaagt ggccgcccat cgtcgacacc gccaagttct acgccgtccg ggacctcatg
      721 gaggccaaca gccggtcgtg ccgcaaccag atgaagaagg tccgccacac ctacatcctg
      781 aaccacggcc tgctcaactg cgggaagtgc aacaccgaga tggaaggcac ctgcgggacc
      841 gggcggctca agacgaagca cttctactac gtctgcaaga accgggaatg ccgtctacgg
      901 atcccggccg gggagatcga aggcctcatc ctggagcggg tcaaagagct ggcgctgagc
      961 ccggacatcc tcgggcggct cgtcaccgag acgaaccagc gcctccagac cgaactcccc
     1021 gacctcaccg agaagagggc ggcgaccgat cgcgacctca aggccgtgac cgccgaagcc
     1081 aagaagctac tggcccgctg gcaggacacg cccgaggccg attcgttcgt ccgcgcccag
     1141 ctggccgacc tggaggaacg acggaaggcg ctcgaactgg cggtggtcga cctagatcaa
     1201 cggctcgccg ccctcggtcg caccacggtc gacgaaggcg tcgtacggaa ggcactggcg
     1261 gcattcgcgg agggattcgc cgggttagag ccctaccggc agaaggcgat gatcgggttg
     1321 gttattcagc aagcgaccgt gagcgacgac tcgttgacgc tctcaattca cggtgagcca
     1381 gtagtaattg gcgaaaaaga aacgtccggg aacgcggtaa acgcgcgccc ggacagcggt
     1441 gcgtggctcc tgaggtagga ctcgaaccta caacccttcg gttaacagcc gaatgctcta
     1501 ccattgagct actcaggatt atgtcgttgg cgggtaaagg tttaacgcga caacgagtga
     1561 aggattatag gggtgtgggc ggggggagtc aattgaattc aggtccgcgg ggtcagcggg
     1621 cggtggcggt cttgacgcgt cttgccgggg cttcggtgcc gccgaaacaa gcccgtgaga
     1681 gcctctcgag ggtgccgggc ttctccgcca gtgtgtcata gggaaagaag cagatcccgt
     1741 cgatgccggc gtcattgagc cggcgggcgg actccacgac cgaggtgagt ggctggttgt
     1801 agacggcgag gcctgcgtac atcaccacgt tgagcggcat ggtcttctgc gcgagcatga
     1861 actggtccat gacggtgctc gacttggggc tgtacatcat gggcaccacg aggtcgagca
     1921 ggccctcttg gcaccacgtc atccaggcct ggccgacgtc gttcggggcg tagttgcagt
     1981 tggggaagac ggcggccgac agcgtgacgg gcttgtcgag cgcctcgagg ccgtcatgga
     2041 tgtcgcgcac caggcgggtc acctggtcgg cgcgccattc cttccagtct ttctcgagct
     2101 gggccaggcc ttcggcgccg taacgggcct tgagggggat ggcggcgttg cggagcttcc
     2161 aggggtccac ccccttttcg gccaggaagc cggctcgtgt gactttgtcg aagccggttt
     2221 cgaggagcgg gttgcggatg taatcgaggt ggatgccgtc gacgtcgtag cgttcggcca
     2281 cttcgagggc catggcacag aggtgcttcc tgacttgtgg attgccgctg gccaggaaca
     2341 caccttcggt gcgggcctgc tggagttcgg tggtggtgta gctggtcatg gggcgccccc
     2401 tggcgtccac ggctacccag tcggggtggc gcttgatcac gtgatccgtg agcttggggt
     2461 cggaatctcc ctgccacacc atgaagacgt tgagccaggc gtgcacttcg atccctttgg
     2521 catgcgcgcg gcggatgagg agatcgagcg ggtccatggg ggtgccgtcg tcgaacgcca
     2581 tgccttcgat ctgcgtggct ttggggacga tgtcggagtt gtaccaggcg tcgccgaggc
     2641 cgcggacctg tacgaacaac acgttgaggc cggcgttggc ggcgtcatcg accgtgcgct
     2701 ggatgcgggc ggggctcatc agctggtcgc gcttgaccca ggcggcgcgg ccgtccgtga
     2761 gggccgggcg atgggaggcc tgggcgctga ttggcaatga ggccagcagc aggatggcga
     2821 gcgcaccggt gaggagacgg ggcatgcggg cgttgagatg ggtcacgcgg gtcatgcccg
     2881 tattatcggc acgctccggt gcgatggata ggggaaaagt gcggttgggg cgcttgtaaa
     2941 cgacggtggc tccacgattt ggcggagctc tcccctgtcc ataatggcca ttcggggacg
     3001 cgggccggtg agggagtggg tggtggttgc ggcaatgaga acgatgcggt cttcggcgag
     3061 ttcggtgagc agctcgagga agggtgtgcg ttcggttgga tcgagggcag tagctggttc
     3121 gtccacgagg atgagcgcgg ggcttccgat gagcgtgaca gccagggcga ggcggcgctg
     3181 cgtggcgagg gtgtgggcgt gggcgtcgtc ggtcatgggc ctgtttcgct gctcccagag
     3241 atcggtcttt cggagcagtt gctcgacggt ggccttgcga aggcgatcat cgatgatgcc
     3301 gctccgcagg gcgacatgat cgagtcgttc ccaggggctc aagcttggat gaacgcccgg
     3361 gtcgtgggat agccaggcga tgctgcggca tgcggcttcc ctgcggttca ggatgtcgat
     3421 gccgttcagg tgcacgctcc ccctgtcggg ctcctggagc gtggcgagga tccggagaag
     3481 cgtggatttg ccggcgccgg tggggccggc aatgacggtg aggccggtgg gaatggtgag
     3541 ggtgatgtct ttcagggcct gggtgccgtt ggcgtaggtc ttggtgaggt tctggatgtg
     3601 gagtggcacg ggctgtgtct ccgggggtgg gggtactcgt tccgggagcg agttgggtga
     3661 aaagaacttt atgatataaa gtgtcggggg cgcaaggggc tccgaatcga ctatttggcg
     3721 ctgaggggga tagaaaacgg gaggcggtgc ttgagcaccg gcctcccgtt tgtttttgct
     3781 cggatttgac gatgtctaag ggctaatagg tgtcgacctt gcttcgcaag ctcgactatt
     3841 aacccttctt gcttgccttg ctggcgcctt tcttctgggc tttctgggcg gcgccggtct
     3901 tgcccctggt gccgggcatg ctcttggtgc gggcttcctt ggtgcccttg gggccgcgcc
     3961 ccttggcctt tctgggagcc tcctccactt ccgcttcgtc ttcggcagcc gccttcttct
     4021 tgccgccctt cttcgttgcg gcttccctgg cggcggcttc ggcggccagg cggtcctggc
     4081 gctccttctc gagctgctcc ttgctcttca ccagttcgaa gatgcccatt tcgccggcat
     4141 cgccgaggcg acggcgggtc ttgagaatcc gcgtgtagcc tccctggcga gtcgtgtacc
     4201 agggcgcgat ggtgtcgaac agcttggtga ggacggtttc gtcgaacagg taactggcca
     4261 cctgacgccg cgcgtgcaga tcaccgcgct tggcgaacgt gatcatccgc tcggccacgc
     4321 ggcgcgcttc cttggccttg gccaccgtgg tctcgatccg ctcgtggcgg aagagttcgg
     4381 tgacgaggtt gcggagcagc gcccggcggt gctcggacgt ccggctcagg tgtcgatggt
     4441 cgcgacggtg tctcatgggt ctagtctacc tttcgctcag ttcttgtcgt cgtcgtccgc
     4501 gggctcggcg tcgtcgtcat ccgaggctgc gggaccgacc ggggtctttt cgtcttcgtc
     4561 gtcgtcggcg tcgctggagg cgtcgatcgt gtcgtcttcc agatcgtcgt cctcgtcgtc
     4621 gtcgtcgtcg tcctcgtaat cgtagtcgtc gtcgtcatcg ccaccgacga gcatgtcggc
     4681 gggaggcgga gggagcggcg cctgcggccc taagtagggc gtaacgtcca tgccgaagcc
     4741 gaggcccatc tcggtcagga tgtccgagat ctccttgagg ctcttccggc cgaagttgcg
     4801 gtacttgagc atctccgatt cgggcttgcg aaccagctcg ccgatggagc ggatattggc
     4861 ggcgcgcagg cagttggccg agcggaccga cagctccagc tcctcgacgt tcttcaagag
     4921 caggtcgcgg atgcgggcca tgtcggcgtc gaccacctcg tcctggtccg tttcgatcgg
     4981 ctcctggaac tgcacgaaga gggcgaagtg gtcctggagg atcttggccg agtaggccac
     5041 ggcgtcctgc gggaggatcg agccgtcggt ccacacctcc accgtcagct tgtcgtagtc
     5101 gacgcgctgg ccgatgcggg tgttctcgac gaagaagttg accttcttga tcggggagaa
     5161 gtgggcgtcc accgggattt cgccgatggg acgatcctct tccacggcgc cttcaccgga
     5221 gaggtagccc cggcccgcgg tgatctccac ctccatgtgg agggtgcctt ccttgttgac
     5281 cgtgcagatg tgctggtcgg ggttcagaac ctcgacctcc gggccatgct cgagatcgcc
     5341 cgcgagcacc tcggccgggc cggccacctt gaagcgcgcg cggctctggt aatccgcgtg
     5401 cagcttgtag cgggtctgct tgaggttgag gatgatctcg gtgacgtctt ccagcacgcc
     5461 cgggatcgtg gagaactcgt gcaggacgcc gtcgatcttg accgacgaga tggccgcgcc
     5521 ctggagcgac gacagcagca cccgccggag cgagttgccg atcgtcatgc cgaaaccgcg
     5581 ctcgagcggc tcgatggtga acttgccgta gcgatcgttg ctgaccgatg aatcgatctc
     5641 gatgcctcgc ggcatctgga gggtcttcca cttcattgat tgcgcctcct cggcgtcttc
     5701 cctgtgaccg ttcgccacca cggcgcggcg tcgacaggct ggtggatcac tggaacgatt
     5761 acttggagta gagctcgacg atcaggtttt cctggacctg ggtcgggatg ccgtcgcgcg
     5821 aggggacccg cgtgatccgg ccggagaagg tcttttcgtt gacctcgatc cagtcggggg
     5881 tttcgccccg gcggccgaca ccctcgaggg cggcctggat gacgacgttc tggcggctct
     5941 tctcgcggac ggcgaccaca tcgcccggct tgacccggta ggacgggatg tcgacgatcc
     6001 ggccgttgac cgtgacgtga cggtgacgga cgagctggcg cgactggctg cgcgaggccg
     6061 cgaagccgag ccggaagaag atgctgtcga gccgcgtttc gagaagctgc agcagcgtct
     6121 cgcccgtgac gccggacgag ccggcggcgg tggcgaagta gttgcggaac tgcttctcca
     6181 tgattccgta gatgcggcgg gccttctgct tctcgcgaag ctgcacgccg tagttggtga
     6241 gcttggtgcg ccggtcacgg ccatgatcgc cgggggcgta ctggcgccgt tcgatggcgc
     6301 acttgtcggt aaagcaccgc tcgcccttca ggaagagctt catcccctcc cggcggcaga
     6361 gacggcagac tgcgtcacga taacgggcca ttgttcctca atcctcctta aacgcgccgc
     6421 ttcttcggcg ggcggcaacc attgtgcgga atcggcgtca cgtcgcggat cgactggatt
     6481 tcaagaccgg cagcctggag gctgcgaacg gcggcctcac gaccggcgcc cggacccttc
     6541 accagcacct cgaccttgcg cagcccgagg cccagggcct cgcgtgcggc attctcggcc
     6601 gccacctgtg cggcgaacgg cgtcgacttg cgggaaccct tgaactgcac cttgccggcc
     6661 gaagcccacg agatgacgtt cccttcaccg tcggtgatcg acacgatcgt gttgttgaac
     6721 gtggccttga tgtgggccac gccgctgatc ccgacgcgct tgtccttctt cttgcgtttt
     6781 tccttcggct tggccacggt ttcctctcct tacttggtcg gcttcttctt gccggccatc
     6841 gtcttcttgg gacccttgcg cgtgcgggca ttggtccgcg tgcgctggcc acgcgtcggc
     6901 aggcccttgc gatggcgcag gccgcggtac gagccgatgt ccttcaaacg cttgatgttc
     6961 atctccactt cggtgtggag ctgtccttcc acgcggtagc gcgtctcgat ctcggtgcgg
     7021 agacgacccg tctcctcttc cgaaagatcc ttgacccggg tgtccgggtt gacgtgcgtg
     7081 gcgcggacga tgttcctcga caggttcggt ccgatgccgt agatgtaggt gagggccacc
     7141 tccacccgct tgcccatcgg cagatcgacg ccggcgatac gagccatggt gctctcccct
     7201 tatccctgcc gctgtttgtg gcgcgggttc ttgcagatca cccgcacgac gcctttgcgc
     7261 cgcacgagct tgcagtgttc gcagatcttc ttgactgatg ctttgacttt catctcatct
     7321 ccggtcgcgc cgcttgtcta cttgtagcgg tagacgatgc gtccgcggga caggtcgtac
     7381 ggtgacaact cgaccgtcac cttgtcgccc gggagaatgc ggatgaaatt catcctcatc
     7441 ttgcccgaga tgtgagccag gacaacgtgc ttgttttcca gttcgacccg gaacatggcg
     7501 ttggggagcg gctcgatgac cgtaccctcg acctggacgc cttcctgttt gctcattcaa
     7561 tccttcgttc cggaagggct gtgcacccga ccggtttcgc gcggtccact caggacaggc
     7621 tcaggatatc cggcccgttc tcggtgaccg ccaccgtatg ctcgaagtgg gccgccagcg
     7681 agcggtcgga cgaaaccacc gtccagccgt ccttgagcgt ttccacttcc catccgccga
     7741 cattcaccat cggttcgatg gcgaacacca tgcccggctt gagcaccact cccgaccccg
     7801 gttgcccgta gttgggaatc tgcggatcct cgtgcaggtc ccggccgatg ccgtgcccca
     7861 ccaggcttcg cacgatggag agcccgtggc tctcgacgtg ggcctggatg gcgctcgata
     7921 catcgcccag gcggttgccc gcccggacct gctcgatgcc cttcaagagc gattccctcg
     7981 tgaccttgag gagctgttcc gcctggccgg tcaccgcccc caccgtgaag gtgcgggccg
     8041 aatcgccgtg aaaaccgttc atcaggacgc cgacgtcgac cgagatgatg tcgccgtcct
     8101 tgagcactct cttgcccggg atgccgtgca cgacctcctc gttgaccgag gcgcagatcg
     8161 tgtggcggta tccgcggtat cccaggaacg acggcgtgcc gccgttcttc cgtatgaatt
     8221 ccatcgccag ccggtcgagc tcgagcgtgg tgacgccggc cttgacgtgc ccggccagtt
     8281 cgtcgagcgt gcggctcacg agcctcgcgc tcccacgcat cgcctcgact tccgagggcg
     8341 acttgattcg aatcatcgtt ctcttcccgc gccggtcacg aggaccggag cgccgcctcg
     8401 acccgcccga gcacttcctc caccgggccg atcccgttga cctcccgaag gagcccccgc
     8461 gagcggtacc agtcgaccag cggccgggtc tgggcgttgt agacactgaa ccggtgacgc
     8521 accgtgtccg gggcgtcgtc ggtgcggagg atgagctccg cttccgggtg atcgtcgcac
     8581 cgacccggca tcctgggcgg ctgcgaggcc aggttgtaaa cccgaccgca caccggacag
     8641 gtgcgccgat tgccgaggcg ttcgaccacg ttctcctcgg gcaccgccag cgacaccacg
     8701 ccgatgagcg gcttgccgat cttcgtcagg atccggtcca ggccttcggc ctgggccagt
     8761 gtcctcggga aaccgtcgag gagaaaaccc ccccctttcc cgttcttcat tcgctccccg
     8821 accatacgca ggatcacgtc gtcggggacc aggtctcccg cgtccatgaa gctgcgggct
     8881 tcccgaccga gcggcgtatc gttcgccact tcggcccgga tcatgtcccc cgtggccacc
     8941 gattggacgc cgaaacgatc cgccagaagc ctggcctgcg ttcccttgcc gacacccggc
     9001 ggtcccagga ggatgaaaat catcggtcag gagctgacgg ctaccgcccg aaacgggcct
     9061 tcagccgccc tttcttcatg aagccgtcgt agtgccgcat cagcatgtgc gactcgatct
     9121 gcgccagggt gtccagggcc acgccgacca cgatgaggag gccggtgccg ccgaagtaga
     9181 agggcacccg gagcaggttg atcatgacgt ccggcaggac cgcgatgaac gccaggaaga
     9241 tggcccccgg cacggtaatg cgggtcagga tgcggtcgat gtattcggcc gtcttctttc
     9301 ccgggcggat gcccggaacg aagccgccgt gcttgcgcat gttgtccgcc aggtcgaccg
     9361 gattcaggac gatggcggtg tagaaatagg tgaagaagat gatggccagc gaatacagga
     9421 tgagatacac ggcgtggtgc ggcgcgaaaa tctgcgcgat gctcgacgcg aactcgttgg
     9481 tccggaagaa gctggcgatg gtgcccggta tcgataccag cgcctgggcg aagatgatcg
     9541 gcatcacgcc ggccgtgttg acccggagcg ggatgtgcgt gttctgccca ccgtacatct
     9601 tgcggcccac catgcgcttg gcgtactgca ccgggatctt ccgctgcccc tgcgtgatca
     9661 ggatggtggc catgatgacc gccagcatga gcgccaggat gaacccgagc ttgacgaact
     9721 tgaggaagtt ccagccggtg gcggcctccg agcggagcgt gttcatgatg tcgagcggat
     9781 agcgggccac gatgccgatg aagatgatga gcgagatgcc gttgccgatg ccccgctcgg
     9841 tgatctgctc gcccagccac atcacgaaca tggtgccggc cgtcagcgtg atgaccgtca
     9901 tcatgacgaa gccggggccc gaatgggaga cgatgggttg gccgtccggc cccgtaaagc
     9961 ccgagctctg gaggaagaag ctggcgccgg ccgcctcgat ggccgccagg aagaccgtgc
    10021 cccagcgggt gtactggttg atcttcttcc gcccttcctc accctctttc tgcagcttct
    10081 cgaagtacgg gatgaccgcc ccgaagagct ggaggatgat ggaagccgaa atgtacggca
    10141 tgatgccgag cgcgaagatc gtggccttcg acaggttgcc gccggcgaac atgtcgtaga
    10201 ggccgaacac cccgttgccc gcctggcgga cgagcgccga gagggcatcg ccattgatcc
    10261 ccggaatggg gatatggctg cccatccggt acacgaccag gacaagcccc gtgaagatga
    10321 gccgcttgcg cagctccggg atggaaaggg cattgcggaa gctcgtgaag aactccccga
    10381 tgcgatcgaa cacggtcagg ccttcgcttt cttggggaaa cgcttcggac gcttgggctt
    10441 ctcgatgacc tggaccgtgc cgccggccgc ctggatcttg gcgatggcgc ccctggtgaa
    10501 ggcatcggcc ttgatggtga gggccttgct gagctcaccg tcgcccagga tcttgagaag
    10561 cttctgcccg cccgagagcc ggccggcctt cttgaggcct gccaggtcga tggtgccggc
    10621 aatcgtcacg tcattgaggt cgcccacgtt gatcgtgtgg tactcgaccc ggaagatatt
    10681 ggtgaagccc accttcggca gtcgccgctg gaggggcatc tgtccgcctt cgaaccaggc
    10741 cgggatgttc gggccggagc gggccttgac gcccttgtga ccgcgcgtcg aggtgccgcc
    10801 atgaccggag cccgtgccga tgccgaggcg cttcttcttc ttgacggcgc ccctggccgg
    10861 cttgaccgtc gtgatggtca ttgccatgtt cagccgacct ccttgactga aatcaggtgc
    10921 ttcaccttgt cgatcatgcc gcggatctgc ggcgtgtcgc ggtgaaccac cgtctggtgc
    10981 atgcgggtaa gcccgagggc ccggatcgtc cgctccatct tgaagccgga cttgatgatg
    11041 ctccggacct gggtgacgtg gatttcctta gccattgctc gcctccgcgg cggcctcggc
    11101 cttcggtgcg aagagttccg agacgtgctt cctgcgcatg cgggccacgt cgccgatctt
    11161 gcggaggcgc gtgagaccgt tgaagaccgc cttcaccgcg ttgtgcgggt tggtcgatcc
    11221 gatggacttg gtcaggacgt cctgcacgcc gcacgcctcg aggacgggac ggacggcgcc
    11281 gccggcgatg actccggtac ccggcgacgc gggcttcagc agcacgcgcc cggcaccgaa
    11341 ctcgccgatg atctcgtggg ggatggtgcc cttcacgagc ggaatgagga ccatgtggcg
    11401 acgggccgcc tcggaggcct tgcggatggc atccgccacc tcgttggcct tgccgagacc
    11461 gatccccacc cgacccatct tgtcaccgac ggtgacgatg gcgttgaagg agaaccggcg
    11521 tccgcccttg accaccttgg cgcagcgatt gatctgcacc acgtgctcga cgtattcgct
    11581 ttcctggctt tcgtagcgcg gtgtcatcgg ctccgtcctc ctagaattcc aggccgccct
    11641 cgcgggcgcc atcggcaaga gccttcacac gaccatggta gagatagccg ccacgatcga
    11701 aacggacccg ggtcactccc ttttcctttg ccttctcggc gagcacgatg ccgacggcct
    11761 tggcgcgccc cgacttgccg gacgcccctt cgaggctccc gatcctggac ttcacgtcca
    11821 ccgacaggct ccccaccgtg aggagggtcg tgccggtgac gtcgtccacg agctggccgt
    11881 agatatgctt gtccgagcgg aagacgcaga ggcgcggaca cgaggcctcg ccgctcatct
    11941 tccggcggat gcgccggtga cgcttttcgc gggccgtgcg ccgatcacgt gaaatgattt
    12001 tcatgatctg cctctttcct taagcgccgg ccgacttgcc ggccttgcga cggatgtact
    12061 cgccttcgta cttgacgccc ttccccttgt acggctcggg cggacggaga cggcggatgt
    12121 tggccgccac ctgccccacg agctgcttgt cgatccccga gatctcgagc cgggtgggac
    12181 tttccacctt ggcctcgatc ccggcgggaa gcgggaattc gatcgaatgc gaaaaaccga
    12241 gctggagcgt gaggctccgg ccggcgagcg cggcgcggta acccgtgccg acgatttcaa
    12301 ggaccttccg gtatccctcg gagacgccga ccacctggtt gttgatcagg gcgcgggtca
    12361 ggccgtggat ggccgggccg gtgcgctctt ccgaggtgtt cacgatctgg agggtgttcc
    12421 cttccatctt gagatcgacc ccggcgagcg gccggtacga gagctcgccc ttgggtccct
    12481 tcacccggac ctcgccgtcc ttccaatcga cggaaacgcc cgaggggagg ttgatgatct
    12541 tcttgccgac gcgggacatg gttcgctccc ccctaccaga tcatgcagag gagctcgccg
    12601 cccagtccgc gcacgcgcgc ttcccggtcg gtgaggattc cctgcgaggt ggaaacgatg
    12661 gcggtgccca tgccgccctg aacgcgcggc accgtgtgcc ggttgacgta gatccggcgc
    12721 ccaggcgagc tgactcgctt gatgcccgtg atgatggact tttcctcgtc gtcgtagcgc
    12781 aggtagatgc gcagcattcc ctgcttgcca tcctccatgc gcttgacgtt ggccacgtag
    12841 ttttcacgca gcagcacgcc ggcgatttcc tgcttgatcc gcgacgcggg cacatcgacc
    12901 cggcggtgcc gggcctgggc ggcgttgcgg atgcaggtca gcatgtcggc gatcggatcg
    12961 ctgacggaca tcggtgtcgc tcctcttcct tacgacgggg gtacgccgcc cgcggcggaa
    13021 ccacgttcgg gtcccggtcg cgaactggac ggcgcctgcc gtcgaattac cagctggcct
    13081 tggtgacgcc gggaatctgc ccgaacaggg cgagatcccg gaaacagatg cggcacaggg
    13141 cgaacttgcg caggaatgcc cgcggccgtc cgcaccgcgt gcagcggttg tgatggcgca
    13201 ccttgaactt cggtgtgctc ttccacctct cgatcatgga cttcttggcc acgatctctc
    13261 cttagctcga gtaacggttg gcggccgaca tctcagcgct cccggaacgg gaagcgcatg
    13321 agcttgagca gttccatgcc ctgctcgtcg tcgggcgctg tggtgacgaa agtgacgtcc
    13381 atcccgcgaa ccttctcgac cttgtcgtag ttgatctcgg gaaagatgat ctgctcctcg
    13441 atgccgaacg agtagttacc ctggccgtcg aacgagcgct ggggcaggcc gcggaagtcg
    13501 cggatgcggg gaatggcgac cgacacgagc cggtcgagga actcccacat ccgggccccg
    13561 cgcagcgtga ccgcgcatcc gatggcaagg ttctcgcgaa gcttgaagtt cgaaatggac
    13621 ttcttggccc ggcgcaccga ggggcgctgc cccgtgatgg ccgtgagatc gttcatggcc
    13681 gcgtccatca gcttggcgtt ctggatggcg tcgccgacac ccatgttgac cacgatcttg
    13741 gtgagtttcg gggcctggtg gcggttcttc agctggaacc gctccatgat ggccgggacg
    13801 accctctcct cgtagtcctt tctcaaccgt ggcgtgctca ttgattactc ccggtcccgt
    13861 ctaccggccc ggccgcggaa gcgtctcgcc ggacttcctg gcgatgcgct cgcggccgcc
    13921 ctcgggggag cgcttcacgc cgatgcgtgt cggctcgccc gtcttcgggt cgaccagctg
    13981 gacgttggac agatggatcg gcgcctcttt ctcctggatg cccccctgcg tgttccgctg
    14041 ggtgggcttg atgtggcgct tcacgaaatt gaccttctcg acgatgacgc ggttcttctc
    14101 gggcatgacc atcaggacct tgcccgtctt acccttgtcg tcgccggcga tgacgatgac
    14161 cgtgtcgccc ttggcgattc gcatgcggcc catggtcaga cgacctcggg agcgagcgaa
    14221 atgatcttca tgaactgctt gtcgcgaagc tcgcgggcca ccggcccgaa gatgcgcgta
    14281 ccgcgcggct cacgcgcgtc gtcgatgatg acgatggcgt tctggtcgaa acgaatgtag
    14341 gtgccgtctt tgcgacggtg ttccttgcgg gtgcggatga ccacgccctt cacgacatcg
    14401 cccttcttga cggtgccgcc ggggatggcg tcctggatcg aggccaccac gatgtcgccg
    14461 atgcgggcgt accgcttgtg gctgccgccc agcacgttga tcatccgggc cacgcgggcg
    14521 ccggaattgt ccgacacctt caaaatgctt ctcatctgga tcatggcgtc gccttcctac
    14581 ttggcccgtt cgacgacttc gacgagccgc caacgcttgg tcctggacaa cgggcgcgtt
    14641 tccatgaagc ggacggtgtc cccttcctgg caatcattct tctcgtcgtg gatgacgaac
    14701 ttcttccggc gacggatata gcggccgtac tcggggtgct tgaccaggcg ctcgatgacg
    14761 acgacggccg acttgtccat cttgctggac atgaccttgc cgacgcgcat cttgcgctgg
    14821 ccgcgctcct cgccggcctc cgtcgtatcg ctggctgtca tgggcgaatt ctccatgtga
    14881 tcaggccccc gccgggcgcg aacccgaccg gtgctccgca aggatggtct tgaggcgggc
    14941 caggtcccgg cggagattgc ggatctccag cgggttctcg agctgcttca tggcgttccg
    15001 gaagcggagg ttgaacagct cctcctccag gcccttgatg cgggtctcga tgtcggtggc
    15061 ctccatcgac cggactttct cgacgttcag atcgcggggc atcttagaac tcctcgcgga
    15121 cgatgaattt cgtcttgatc ggaagcttgg cggcgccgag cttcagcgcc tgctgcgcca
    15181 gggccgattc gatcccctgg agctcgaaca tgacacggcc gggcttgacc acggccaccc
    15241 agaactccgg gttgcccttg cccttgccca tgcgggtctc ggccggcttc accgtgatcg
    15301 gcttgtccgg gaagacgcga atccacatct tgccgccgcg cttgatgtga cgggtgatgg
    15361 ccacacgcgc cgcctcgatc tggcggttgg tgacccacgc cggctccagg gcctggaggc
    15421 cgaagtcgcc gaaggcgatg gtgccgcccc ggtgcgcctt gccccgcatg cgcccgcgct
    15481 gctgcttgcg gtgtttgact ctgctcggaa gaagcatgga tccctactcc tatcgggcgc
    15541 cgccggccgg agcctggtcg cgatcgcgac cgccccgtcc gccatcgcgg ttgtctcgtc
    15601 cgtcacgccc gcggccgccg tcgcgacctg agtcacgacc agcgccttcg cgcccggctt
    15661 cccgaccgcc gcgcggccgc tccgcctgcg cctcgccgag ttcggcggga cggttctcga
    15721 ggacttcgcc atggaagatc cacaccttga caccgcaggt gccgaaggtc gtgtacgccg
    15781 tggcgatggc gtagtcgata tccgcgcgga gcgtgtgcag cggcacccgt ccctcgcggt
    15841 acgtttcgaa gcgcgccatt tccgaaccac cgaggcggcc ggagcaggcc acgcggactc
    15901 ccagggcccc gctcttcatg gacgcctgga tggccttctt catggcgcgg cggaaggaga
    15961 cgcgtccctc gagctggcgg gcgatgtgca tggccaccag ctgcgcgtcg agatcgggct
    16021 tcttgatctc gtcgatgttg aggtagatct ccttgccggt gatgtgatgc agctcgtccc
    16081 gcagctggtc cacctgggcg ccgcgccggc cgatgaccag gcccggcttg gcggtggcga
    16141 tgttgatcgt catcttgttg gaagcgcgct ggatgatgat cttgctgacg ccggcctggg
    16201 caaggcgctg gcggatgtac ttccgcacct tcacgtcttc gccgaggctg gtggcgtact
    16261 ccttcttgcg ggcgaaccac cgggagtccc aggtccggat gatccccagc cgcatggcga
    16321 ttgggtttgt tttctgaccc atgaatcctc cccttacttc tttccggccg cggcagcgcg
    16381 ggcgggcgac tttgccttca cggccttttt ggccgcggtc ttcttcttcg gagcggcctt
    16441 cgaagccgcg gccttcgtgg cccccgactt cgccgctgtt ttgggtgccg ccttgggggc
    16501 ggcggtcgtg gccggcgtcg gggcggccac ggccgccccc ttccgggcct tggtcccgac
    16561 gatgatcgtg atgtggcacg accgcttgag cagcggcgtg gcgcgaccct gggcccgcgg
    16621 aaggaagcgc ttcatgatcg gggcgggccc gcagaggcac tccttcacga acaggtcttc
    16681 cgtgtggagg cgggcctcct ggacggtggc attggccacc gccgacttca gcaccttgag
    16741 gaccgtccgg gccacgtgct tcgtcgagaa ctggagcgtc tgcatggcgt tgtcgacgct
    16801 cttgccccgc accaggtcga ggacctggtt ggtcttgcgc gtcgaaccac gcacgtaacg
    16861 ggcgatcgcc ttggcttcca tcgtctgctt ctccatcgcg ctccccctta cttcttcggc
    16921 ggacccggct tctgggcggc tttgtcgccc gaggccgagt ggctcttgaa ggtgcgcgtg
    16981 ccggcgaact cgcccagctt gtgcccgacc atgttttccg tgacgtagat cgggatgaac
    17041 ttggtgccgt tgtggacggc cagcgtgtgg ccgacgaagt ccggcgagat cgtcgagcgg
    17101 cgggcccagg tcttgaggac cttcttctcg ccgcggcgat tcatcgcctc gatcttgagc
    17161 atcagcttcg ggtcgatgaa cggacccttc ttgagtgaac gcgccatctc gtcagccccc
    17221 tctacttctt ccggcgccgc acgatgaggc ggtccgacgg cttggtcttg cgggtcttgt
    17281 aacccttggt cggcaggccc cacggagagc agggatgccg gccgcccgac gtgcggcctt
    17341 caccgccgcc catcgggtga tcgaccggat tcatcgagac gccgcggttg tgcgggcggc
    17401 gaccggccca gcgcacgcgc ccggcctttc cgatgacgat gttttcatga tcgagattgc
    17461 cgacctgccc gatcgtgccg tagcactcct ggtggaagat gcgaacctcg cccgacggca
    17521 gcttgacctg ggcgtgggtt ccttccttgg ccaccagctg gcaggccgcc ccggcgccgc
    17581 gggccacctg gccgccgcgt cccgggatga gctccaggtt gtggatgttg gtgccgagcg
    17641 ggatggcccg gagcggcaac gcgttgccca cccggatttc cacctcggga ccggcctcga
    17701 gcctgtcgcc caccttcagg ccgtccggcg ccacgatgta gcgcttctcg ccgtcggcgt
    17761 acacgagcag ggcgatgcgc gccgtgcggt tcggatcgta ttcgatcgag tcgacgcggc
    17821 cggggatgcc ccgcttgtcg cgacggaaat cgacgatccg gtagcggcgc ttgtggccgc
    17881 cgccgcggta ccaggtggac gtgtgcccct tgttgtcgcg gccgcccgtg gaacggagcg
    17941 gctcgagcag gctcttttcc ggctcggtgc gcgtgatgtc cgcgaaatcg gacaccgtct
    18001 tgtaccgaag gctcggtgtc agcggtctga attttttgat gcccatcgtt gactcccggc
    18061 cggcacggcg cctcgcggcc cgcccggctg tcggaaacta gatctgctcg aagagctcga
    18121 ttgcctcgcc ggccttgacg gtcacgatcg ccttcttcca gtcgggccgg tagccggcga
    18181 acttgcccat ccgcttctgc ttgcccggca cgttcatcgt ccggacttcc accacgtgaa
    18241 cgttgaaaat cgactcgacg gccttgcgga tctcgatctt gttggcgccg gcgtcgacct
    18301 ggaacacgta cttgttcgac ttctcgcgca tgaccgtgcc cttttccgtg atcagggccc
    18361 ggacgaccat gttgtgtgcg gacttcattc ggccaccccc ttcagcactt cgagggcgcc
    18421 gttctggatg acgatggcgt cggtggcgag cacgtcgtac accgagatct ggtccgccgg
    18481 gatgcagcgg gcgccggcga tgttgcggac cgagcggtag agcgtgatat cgggtccctt
    18541 gccgacgtac aggacgcgct tgtcggagag agccagcgtc ttgaagagcc cggcgatcgt
    18601 cttcgtcttc ggcgcgtcga gggtcaggtc gtccaccacg tagacgaggc cgtccttggc
    18661 ccgctccgac aggatggagc ggatggccag ccggcgcatc ttcttgggaa tctggtagct
    18721 gtagtctcgc ggctgcggac cgaacacggt gccaccaccc acccagatcg gcgaacgggt
    18781 ggatccgtga cgggcccggc cggtgccctt ctggcgccac ggcttcttgc cgccgccgga
    18841 gacgagcgaa cgggtcttgg tggccacggt gccctggcgc tggttggcgc ggtaggaacg
    18901 gagggcgtcg tacaccacgt cgggccggag cggcgcgccg aaggcggctt ccggcagctc
    18961 aacggtgcct ttttcctggc ccgccgccga gaaatgcttt gcagtggcca ttacttgcct
    19021 cccttgactg cgtcgccggg agcgctcttg atcatgaccc aggcgttggt gtgacccggc
    19081 acggcgccac gcacccagat gaggttttcg tcgaggtcga tgccgaccac cttcaggttc
    19141 ttgacggtga tctgcttggc gcccatgtgg ccggggaggc gctttccctt gatgacgcgc
    19201 gaggggtagg ccgaggcgcc gatggagccg ggctgcttct tggtcttgca gccgtgggcg
    19261 ttgtcgccgc ccgagaagtg gtggcggcgg acgacgccgg cgaagccgcg tcccttggag
    19321 atgccggtga cgtcgacctt ggcgccgacc gcgaagcgat cgaccgtgac gttgccgccg
    19381 acctcgaggc cctccatgac ggggacctcg cgcaggaggc gcttgggcgc ctgtccggcc
    19441 ttcttgaact ggccgagccg cggcttcgac atcaccttct cgcggatgtc gccatacgcg
    19501 atctgcaggg cgtcgtagcc gtgcttgtcg gccgtgcgct tgcccaccac ggggcagggg
    19561 ccggcttcca atacggtgac cggtacgaca cgcccatcct ctgagtagag ctgggtcatg
    19621 ccgatcttcc ggccgatgat ggcactcatc gttcagttcc tccgtcctgg cggaacgtta
    19681 ccgggcgaaa ccccgggcgt tcacagaaca cttgaagcgg cgcggccatc cggccgcacc
    19741 acgttactgt ttgatctaca gcttgatatc gatgtcgacg cccgccggaa gatccagctt
    19801 catgagggcg tcaatggtct gggccgtgct ttccgagatg tcgatcaggc gcttgtggat
    19861 ccgcatctcg aactgctcac gcgacttctt gtcgacgtgc ggcgaacggt tgaccgtgaa
    19921 gaccgacttg cgggtcggca gcggcaccgg accgaccgtc ttggccccgg tccggcgggc
    19981 tgtcttcacg atctccccgg ccgacaggtc gaggatggcg tggtcgtagc tccgcagacg
    20041 aatccgaatg cgctgggcgg acatgttcgt tcccttccct tattccagga tcttgctgac
    20101 gacgccggcg cccacggtgc gcccaccctc gcggatggcg aaccggagac cttcgtccat
    20161 cgcgatcggc gtgatcagtt cgatcgacat cttcacgttg tc
//
LOCUS       DASVOD010000353         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_442274, whole genome shotgun sequence.
ACCESSION   DASVOD010000353 DASVOD010000000
VERSION     DASVOD010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442274"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF720_08170"
     CDS             complement(<1..559)
                     /locus_tag="VF720_08170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879370.1"
                     /translation="MNNLAAYYARRAAEYEKIYDRPERQDDLARLERFLVEAAGGHDI
                     LELACGTGWWTERMARSARTITAIDSVPEVLERARARGCPPGKVRFQGGDAFRPEEIG
                     GSYSLVVTAFLWSHIPRREIPGFLEGIARRFPDGIRVIFADNRFAEGSSTAISRRDDF
                     GNTWQRRRLEDGSEFEIVKNFPTPEE"
     gene            642..1349
                     /locus_tag="VF720_08175"
     CDS             642..1349
                     /locus_tag="VF720_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879371.1"
                     /translation="MIRRERLFQSACWLLLYVLGSVLVSHAGDPPPLDTGGSSTEPSA
                     PAGVGGAITGSGLERGTSIMPELPAKMPEADALSEEQLDAPFAFERMKLLAGTWEGTA
                     GKGDEVVPATIRYEVTAGGTVLVQRLFPGSEGEMMTIFNLDGNDLVLTHYDAKGNQPR
                     MRFDAAESASNLYTFRFNGGTNLGKSTKHVHAGTIHFVNANQIKAEWAVYAGSKKVGT
                     SVFELKRTDSATSVGSR"
     gene            complement(1357..>2022)
                     /locus_tag="VF720_08180"
     CDS             complement(1357..>2022)
                     /locus_tag="VF720_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7879372.1"
                     /translation="GSGARPEYVKRALEMSLDRLRTDHLDLYQIHEPDLVTPIGETLS
                     ALNDLVQAGKVKEIGCSNFSVAQLREAEAAAAPGAARFASVQNEYSLFKREAEADVLP
                     ECERTGMAFLPFFPLASGLLTGKYRRGSIPVSGSRLVDSERWRKTLTDEKLDKVEALV
                     RYAERRGHTLLELAFGYLLARTPVASVIAGATSREQVMANTGAAGWILNEQESAEVDQ
                     ILA"
BASE COUNT          329 a          682 c          673 g          338 t
ORIGIN      
        1 gctcctcggg agtggggaag ttcttcacga tctcgaattc cgagccgtcc tcgagacgcc
       61 gccgctgcca ggtgttgccg aagtcgtcgc gccgcgagat cgcggtggag cttccctccg
      121 cgaagcggtt gtcggcgaag atcacccgga tgccgtcggg aaagcgccgc gcgatcccct
      181 ccaggaaccc cggaatctcc cggcggggga tgtgggacca gaggaaggcc gtcaccacga
      241 gcgagtagga accgccgatc tcttcggggc ggaaggcgtc tcccccctgg aagcgcacct
      301 tgcccggcgg acacccgcgc gcgcgggccc gctcgagcac ctccggcacc gagtcgatcg
      361 cggtgatcgt ccgggcgctc cgggccatcc gctcggtcca ccagccggtg ccgcaggcca
      421 gctcgaggat gtcgtgcccc cccgcggcct ccaccaggaa cctttccagc cgggccaggt
      481 cgtcctgccg ctccggccga tcgtaaattt tttcgtactc cgcggcgcgg cgggcatagt
      541 aagcggccag gttgttcatg gccggggatg ataaggcttc cggcggcccg gccggctccc
      601 tatactcctt tcgggttgga atacatggaa ggaaccattc gatgatccgt cgtgaacgcc
      661 tgtttcaatc agcctgttgg ctcctgttgt acgtcctcgg ttctgtcctc gtctcgcacg
      721 ctggcgatcc gccgcccctc gataccgggg gctcctccac cgaaccgtcg gccccggccg
      781 gcgtgggagg ggcgatcacc ggcagcggtc tcgagcgcgg tacctcgatc atgccggagt
      841 tgccggccaa gatgccggaa gccgatgccc tctccgagga gcagctcgat gcccccttcg
      901 cgttcgagcg gatgaagctg ctggccggca cctgggaagg gaccgcgggg aagggagacg
      961 aagtcgtacc cgccacgatc cgctatgaag tgaccgccgg tggcaccgtg ctcgtgcagc
     1021 gcctcttccc ggggagcgag ggcgagatga tgaccatctt caacctggac ggaaacgacc
     1081 tggtcctcac gcactacgac gccaagggaa accagccccg catgcgcttc gacgccgccg
     1141 aatccgcttc gaacctttac acgttccggt tcaacggggg gacgaacctc ggcaagagca
     1201 caaagcatgt gcacgcgggc acgatccact tcgtcaacgc caaccagatc aaggcggagt
     1261 gggcggtgta cgcaggttcg aagaaagtgg ggacgagcgt cttcgagctg aagcggaccg
     1321 actcggccac gtcggtcggt tcgcgctgac gcgcggtcag gcgaggatct gatcgacctc
     1381 ggccgattcc tgttcgttca ggatccaccc cgcggcgccc gtgttcgcca tgacctgttc
     1441 gcgtgaggtg gcgccggcga tgaccgacgc caccggcgtc cgcgccagca aatagccgaa
     1501 cgccagttcg agcagcgtat ggccgcggcg ttcggcgtag cgcaccagcg cctccacctt
     1561 gtcgagcttc tcgtcggtca gcgtcttccg ccaccgctcg ctgtcgacga ggcgcgagcc
     1621 gctcacgggg atggagccac ggcggtactt gccggtcaac aggccgctgg ccagcgggaa
     1681 gaacgggagg aacgccatgc cggtccgctc gcactcggga aggacatcag cttccgcctc
     1741 gcgcttgaag aggctgtatt cgttctgcac gctcgcgaag cgggcggcgc cgggcgcggc
     1801 agcggcctcg gcttcgcgga gctgggccac gctgaagttc gagcagccga tctccttcac
     1861 cttgccggcc tgaaccaggt cgttcagcgc ggagagcgtt tcgccgatcg gcgtgaccag
     1921 gtcgggttcg tggatctggt agaggtccaa gtgatcggtg cgcaggcgat ccaggctcat
     1981 ttcgagcgcc cgcttcacgt attcgggccg ggctcccgaa cc
//
LOCUS       DASVOD010000354         2588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_597466, whole genome shotgun sequence.
ACCESSION   DASVOD010000354 DASVOD010000000
VERSION     DASVOD010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597466"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VF720_08185"
     CDS             <1..652
                     /locus_tag="VF720_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7879373.1"
                     /translation="LAATTAASWEDCFNAHLYRAIDLRVRPQEDGVERELRISSALSA
                     GLGMIRALDTSLLGYDRGRGFYRRFTDYYPTMLEEMAGLEARVRVERPRLGLKVAPTG
                     SGLRIDDILKGHSADGSLLKVGDVIVEADGRPVMSEERLAEIVQSHRIGAVLPLVIER
                     NGASRSIDLKLSKGRIEYEFFRPAPPEAELGAPNDSTGTSTPPATKDRVPAGRSGR"
     gene            703..2160
                     /locus_tag="VF720_08190"
     CDS             703..2160
                     /locus_tag="VF720_08190"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEX7879374.1"
                     /translation="MRALHWFRKDLRLDDNTALAAACAESNGDVVTAYISEPTILSRP
                     DMAAVRMRFVLESLADLSRALGDAGGGLILRHGDAAEELARLARETGANVVHANAEYE
                     PDLAVRDRQVMDRLAADGVRVQWHHDRFLVAPGSVMNAAGEPFVVYTPFRRACEARGL
                     PPPAPGVTRLPEPRDTAGAPLTSRRLATLQDLGMTTDQDHWPGGAAAGEAALARFIGK
                     PLFDYIPDRDRLDRAGTSQLSHHLRFGTLSPRRVVHETSRALESGAPGAAARNSVEHF
                     ISELRWRDFYGHVMHHFPHVVSGAFRKPYDSLRWVNDDAAFAAWCAGQTGYPVVDAAM
                     RQLARTGWMHNRARMIVASFLTKDLLVDWRRGERWFMNHLVDGDPASNNGGWQWAAGT
                     GTDAQPYFRIFNPVLQGKRFDPDGAYVRKWVPELSGLSADLIHEPWSARPMDLAAAGV
                     ALGETYPEPICDHAERRKLALELYGEARENGRNDA"
     gene            complement(2187..>2588)
                     /locus_tag="VF720_08195"
     CDS             complement(2187..>2588)
                     /locus_tag="VF720_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879375.1"
                     /translation="FNQSGTKTATDIFFPSEDLSFTSDLGLYINDDGFSNPANMHAFI
                     QFGGVPANGRQGVAVQAGLWTNNAFVPNPSVGQSVELCGNGDATMVSSWVATLSNTIG
                     TMNGCGVAVEGVSWARVKSLFDPHKPGFRLN"
BASE COUNT          431 a          871 c          879 g          407 t
ORIGIN      
        1 gctggcggcc acgacggccg cgagctggga agactgcttc aacgctcacc tctatcgcgc
       61 catcgatctt cgcgtgcggc cgcaggaaga cggcgtggaa cgcgagctcc ggatttcatc
      121 ggcactctcc gcggggctcg ggatgatccg cgccctggat acctcgctcc tcggctacga
      181 ccgcgggcgc ggcttctacc gccgattcac cgactactat ccgacgatgc tcgaggagat
      241 ggccgggctc gaggcgcggg tgcgggtgga gcgcccccgg ctgggcctca aggtcgcgcc
      301 gaccggatcg gggctgcgga tcgacgacat tctgaaaggt cattcggccg acgggtcact
      361 cctcaaggtg ggtgacgtca ttgtcgaagc ggatggtcgc cccgtgatga gcgaggagag
      421 gctggcggag atcgtgcaat cgcatcggat cggggcggtg ctgcccctcg tgatcgagcg
      481 gaacggcgcc agccggtcga tcgacctcaa gctgtcgaag ggacggatcg agtacgagtt
      541 cttccgtccc gcgccgccgg aagccgaact cggcgcaccg aacgactcga ccggaacgtc
      601 aacgccgccc gccacgaagg atcgggtgcc cgccggccgc tcgggccggt aacccgccga
      661 ctttcccgcc gccactcaca gtcgaatccc aaggagccgt cggtgcgcgc cctccactgg
      721 tttcgcaagg acctccgcct cgacgacaac accgcgctgg ccgccgcgtg tgccgagtcg
      781 aatggcgatg tggtcacggc ctacatcagc gagccgacca tcctctcacg ccccgacatg
      841 gccgccgtgc ggatgcgctt cgtgctggag agcctggccg acctgtcgcg cgcgctgggc
      901 gatgccggcg gcgggttgat ccttcgacac ggcgatgccg ccgaagagct ggcccgcctg
      961 gcccgcgaga cgggcgccaa cgtcgtgcat gccaatgccg aatacgaacc ggacctggcc
     1021 gtccgcgatc ggcaggtgat ggaccgcctg gcggccgacg gcgtgcgcgt tcagtggcac
     1081 cacgatcgct tcctcgtggc accgggctcg gtgatgaacg ccgcgggaga gccgttcgtg
     1141 gtgtacacgc ccttccgccg cgcctgcgag gcgcgcggat tgccgccacc ggcacccggg
     1201 gtcacacgcc tgccggaacc gcgcgacacc gcaggtgccc cgctgacctc ccgtcgtctt
     1261 gccacgctcc aggacctcgg gatgaccacc gaccaggatc actggccggg aggcgccgcg
     1321 gcgggagaag cggcgctcgc gcgattcatc gggaagccgc tcttcgacta cattcccgat
     1381 cgcgatcgac tggaccgggc ggggacctcg cagctctccc accatctccg cttcggcacg
     1441 ctctcgccgc gacgggtggt gcacgaaacc tcccgcgcgc tcgagtcggg cgcgcccggc
     1501 gcggcggccc gtaacagcgt cgagcacttc atctcggaac tacggtggcg cgacttctac
     1561 ggacacgtga tgcatcactt cccgcatgtc gtgtccggcg cgttccgcaa gccctacgac
     1621 agtttgcgct gggtcaatga cgacgccgcg ttcgcagcat ggtgcgcggg gcagaccggt
     1681 tacccggtgg tggatgccgc catgcgccag ctggccagaa ccggctggat gcacaaccgt
     1741 gctcgcatga tcgtggcctc gttcctcacc aaggatctcc tcgtggactg gcggcgcggc
     1801 gagcggtggt tcatgaacca cctggttgac ggcgatcccg catcgaacaa cggcggctgg
     1861 cagtgggcgg ccggcaccgg caccgacgcg cagccgtact tccgcatctt caacccggtg
     1921 ctgcagggaa aacggttcga tccggacggc gcctatgtcc ggaaatgggt ccccgagctg
     1981 tcagggcttt ccgcggatct catccacgaa ccatggagcg cgcggccgat ggacctggcg
     2041 gccgcaggag tggcgctcgg tgagacctat ccggagccga tctgcgacca cgcggaacga
     2101 cggaagctgg cgctggagct gtacggagag gcaagggaaa acggcaggaa cgacgcctga
     2161 agtactcgga tgggagtgga gccgccctag ttgagccgga agccgggctt gtggggatcg
     2221 aagagcgact tgaccctggc ccacgacacg ccttcgacgg ccacgccgca gccgttcatc
     2281 gtgccgatgg tgttcgagag cgtggccacc cacgaactga ccatcgtggc atcgccgtta
     2341 ccgcacagtt ccaccgattg accgacggac ggattgggga cgaacgcatt gttcgtccag
     2401 aggcccgcct gcacggccac gccctggcgc ccgttggcgg gcacgccacc gaactggatg
     2461 aacgcatgca tgttggccgg gttcgaaaag ccgtcatcgt tgatgtagag accgaggtcc
     2521 gaggtgaagc tcaggtcttc actgggaaag aagatgtcgg tggctgtctt ggtgccgctc
     2581 tggttgaa
//
LOCUS       DASVOD010000355         8022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_494455, whole genome shotgun sequence.
ACCESSION   DASVOD010000355 DASVOD010000000
VERSION     DASVOD010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8022
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494455"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            569..814
                     /locus_tag="VF720_08200"
     CDS             569..814
                     /locus_tag="VF720_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879376.1"
                     /translation="MLRRVPDRRGLAASLLALSLVTTLSLGGCGTDAATAPEKPGGNP
                     QDIGGPLFGDEQNTPKDNDKKMEDGSQLDYDTNDTAR"
     gene            complement(938..1393)
                     /locus_tag="VF720_08205"
     CDS             complement(938..1393)
                     /locus_tag="VF720_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879377.1"
                     /translation="MHRIEDAPMQLRSERHLPTAPRFRAGLIVATSMKHSLAGCFIVA
                     ALTIWTSSPVHAAGNINAKIMLNALSLTTKNPCTRALNLPLACTGYEAGTSNLALYPT
                     TLSVSIDYSGIGEAAYYCVKAVTVEGVVPMTPTTWTGIKSLTRQGVTKP"
     gene            complement(1404..2282)
                     /locus_tag="VF720_08210"
     CDS             complement(1404..2282)
                     /locus_tag="VF720_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879378.1"
                     /translation="MLHALPLTTKNFCTRTANLPSGGCAVYSLGVNNVLLYPPGQFVY
                     ALVVNGSQTEGIGGAQFGIDYNGLPQTGLEVWGWTNCGTSELPMAGWPAPGTGNLVSF
                     SVPANCQTGGSTSLGAVATLGYSYCGAYTPDVMRITPHPIRGKAVVANCAAVEDELFD
                     STDPRCTALGAVGFARPGINPCGNLFQGCFLNCSIEGPGVVTSGQTGIEYDSSVTFGS
                     RSWTIIGNGTFDQPPDGHIGVLVTAGAPGQFTLKLNVSDEHTNLSCERTVTVVDAVPT
                     IPASWGRIKALSLIDR"
     gene            2672..3865
                     /locus_tag="VF720_08215"
     CDS             2672..3865
                     /locus_tag="VF720_08215"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7879379.1"
                     /translation="MNPTATMTMPIADAMGRLGTETAFEVLARARALEAQGRDIVHLE
                     IGEPDFDTPKHIIAEAHAALDAGYTHYGPSAGLPELREAIAAHVVKTRGVPTEAAQVV
                     VVPGAKPVMFYGMMATVNPGDEVIYPNPGFPIYESVIKYLGAKPVALPLQESREFRFD
                     LADLKARINERTKMVVVNTPQNPTGGVLTKDDIAGIVELVRGRNIWVLSDEIYSDLLY
                     EGDHVSLYAQAGMPEQSILLDGFSKTYAMTGWRLGYGVMPKDLAVHVARLATNCNSCV
                     TSFVQRAGVAALTGPRDEVDAMKAEFHRRRDWIVQGLNAIPGLSCVRPHGAFYVFPNI
                     TKTGLTSEEMARRCLDEAGVAVLSGTAFGAHGQGYLRLSYANSLPNIEKALERLDALC
                     RSLVG"
     gene            3913..4839
                     /locus_tag="VF720_08220"
     CDS             3913..4839
                     /locus_tag="VF720_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX7879380.1"
                     /translation="MIALGGNALLPAHGSGTIDEQIAITRRTMRVVARLALPGVRLVV
                     THGNGPVVGNILVRNLAAADRIAPMPLDVCGADSQGGLGYMIQQTLENELFLLGRHRP
                     VVTLVSQVRVDGRDPAFEHADKPIGPFVTAGEAERLRAEHGWEMVQDAGRGWRRVVPS
                     PIPVEIVEEQAIRTLRDSGFIVIALGGGGVPVIRRDDGTLEGVEAVIDKDRASAVLAR
                     DLRIPSLVIVTAVDRVRLGFGTPDEQPLSAMTLAEARLYLAEGQFGAGSMGPKIEAVC
                     DFLDAGGREAILCAPDQLAEALGGEAGTRVTR"
     gene            4932..5555
                     /locus_tag="VF720_08225"
     CDS             4932..5555
                     /locus_tag="VF720_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7879381.1"
                     /translation="MFARFISLVAVLCVAAAPVSAEVLLNEMLADPARDWNGDGTTSS
                     RDDEWTEIINTGPGVADLTGFRLAGPDSVWRYEFTGSLAPGAVKVVYGRQSYDWEVAN
                     GFPAFGLRLGNSGGELVLFRVAGATVTQIDCYAYTDHEADDDRSSGRGPDGSPNWILL
                     DGHNPWTGTAEPKGTGCLPTPGARLTCATPVEAVTWGRVKALIDLDR"
     gene            complement(5668..5754)
                     /locus_tag="VF720_08230"
     tRNA            complement(5668..5754)
                     /locus_tag="VF720_08230"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5718..5720),aa:Leu,seq:cag)
     gene            5904..6521
                     /locus_tag="VF720_08235"
     CDS             5904..6521
                     /locus_tag="VF720_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015131062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX7879382.1"
                     /translation="MHFLDPRHLIESFGAFAYIGLFAVVFAESGLFFGFFLPGDSLLL
                     TAGLFAYKGFLDIKILLPLLFVATVLGDQVGYWFGRKVGPPLFKRNESLLFRPRNLAA
                     ARSFYEKHGGKTIILAKFMPFIRTFAPIVAGAVAMPWRRFTMFSLTGGLVWAIGLTLA
                     GYLLGRAFPPEVLDRYFIVIVGLVIVVSVAPSVVHVWKEHRARSA"
     gene            6523..7017
                     /gene="smpB"
                     /locus_tag="VF720_08240"
     CDS             6523..7017
                     /gene="smpB"
                     /locus_tag="VF720_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX7879383.1"
                     /translation="MTAGKAPPGAERGGEIKVVCTNRKARHLYHILDSWEAGLILTGT
                     EVKSLRAGHATMTDAYAALQGMEMILHNLHINPYEQGNRYNHQPTRPRKLLLHRRELS
                     KLVGRTVEKGLTLVPLQVYFRGGWAKVEIALARGKKIYDKRRDMADREMKRDVERSLK
                     ERSR"
     gene            7140..>8022
                     /locus_tag="VF720_08245"
     CDS             7140..>8022
                     /locus_tag="VF720_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879384.1"
                     /translation="MYGDGRPEEQAAILEAGPRTWLSARDLSAVIGAPRYWRSDLKRL
                     VIRTPTHEMALVVDSDVAILDKTTPLHLPAPVLFVADGVWIPIELLLDADRYDFGVNG
                     LGGASPWFDLPVRWDAAARRLDAGTPGARLTAITSPADDPGRLDILVDGAWEWRLAAA
                     DRARMVLRLIRVGLADSVATPAASDFISGLSATPIPEGIEIGFTPGALAVGYRVQRLD
                     RPSRLSVLVSSDPADLATGRVTPFARPEGADVPVFGQETAREGGVRTIVLDPAGGGSA
                     GIRAKGTSESTAMLELAQ"
BASE COUNT         1378 a         2632 c         2666 g         1346 t
ORIGIN      
        1 tcctcccgtc tggcaacgct cgggcgtcgt gaatgagatc gtgtttcccg tacccggact
       61 gggccagcct gccgtaggaa gttccgagtc ggcacagagc gaccacgaat agatgtcgac
      121 gcccgagaga agggcgccgt cgtaattgat cccgaaggac gcaccgctca ttccctgaac
      181 ccgacttccg tccacgatca acggataaac gaagtacacc tgcgggtaga ggccgaggcg
      241 gtgggctcag gctgggccaa ctgctgccgg aaagtatagc agccgttcca ggaaggaacg
      301 atcgagaaac attatccagc acgggcataa tttggatgca gggcgtgtcg ccgccgactt
      361 cgggcaatta tgggcgtcca ccaatcgaca ggtgtccccc ggggggtcat gacacccgga
      421 gcaatttacc tcacgtgggc acggctttca cggcggatcc cgtggcatgg ccgttgcact
      481 acacccatca cgcacagatc acggtcaatg gcgacgcgca ccggcgaacg agccgtcacc
      541 gtttacacca ctttgcagga ggttcgtcat gctacgcaga gttcccgacc gtcgcggcct
      601 tgccgcgagc ttgctcgccc tctcactcgt cacgactctt tcgctcggtg gatgcgggac
      661 cgatgccgcc acggcccctg aaaaacccgg cggcaatccc caggacatcg ggggtccgct
      721 gttcggtgat gagcagaaca cgcccaagga caacgacaag aaaatggagg atggaagcca
      781 gcttgattac gacaccaacg acaccgcccg ctgacgcgga ttcgaaactg ttctcgatag
      841 aatgatggcc aactcggttc gaaccgagtt ggcctcactg tttgatggtc agaacgtcgt
      901 tcccgggagg ccgatcaagg ccgaccggat gaggtattca aggcttggtc acaccttgcc
      961 tcgtcagcga cttgatcccc gtccacgtgg tcggtgtcat tggaacaact ccttccaccg
     1021 tcacggcctt gacgcagtaa taggcggctt cgccaattcc cgaatagtcg atcgacacac
     1081 tgagtgtggt cggatagagc gcgaggttgc tggtgccggc ctcgtagccg gtacacgcca
     1141 acggaaggtt caatgcccgg gtacaagggt tcttggtggt caggctcagg gcgttcagca
     1201 tgatcttggc gttgatgttg cctgcggcgt gaaccgggga cgatgtccag atggtgagtg
     1261 cggcgacaat gaaacaacct gccagggaat gcttcattga ggtcgcgacg atcagcccgg
     1321 ctcgaaaacg tggtgcagtt gggagatggc gctccgatct caattgcatc ggcgcgtcct
     1381 cgattcgatg cacggccggc tgactaccgg tcaatcaatg aaagtgcctt gatccgtccc
     1441 caactcgccg gaattgtcgg cacggcgtcc acgacggtga cggttctctc gcacgatagg
     1501 ttggtgtgct catcactgac attgagcttt aacgtgaatt gtcccggggc tcccgcggtc
     1561 accagtacac caatgtggcc atccggaggt tggtcgaacg tgccgttacc gatgatcgtc
     1621 cacgaccgcg aaccgaacgt gacggaggag tcatattcga tgcctgtctg accggaggtg
     1681 acgacgcccg ggccctcgat cgaacaattg agaaagcagc cctggaacag gttgccgcac
     1741 ggattgatcc cgggtcgggc aaagcccacg gctccaagcg cggtgcagcg cgggtccgtg
     1801 gaatcgaaga gctcatcctc gacggccgcg cagttggcca ccacggcctt ccccctgatt
     1861 ggatgagggg tgatgcgcat gacgtcgggc gtgtaggcgc cgcaataaga gtatccaagt
     1921 gttgccacgg ccccaaggct ggtgctacct ccggtctggc aatttgccgg cacgctgaag
     1981 ctcacgaggt ttcccgttcc gggcgccggc cagccggcca tcggcaattc gctcgttcca
     2041 cagttcgtcc aaccccagac ctccagcccg gtttgcggca gcccgttgta atcaatgccg
     2101 aattgggcgc caccgatccc ttcggtctgg gagccattca cgaccagggc atagacgaac
     2161 tggccggggg gatagagcag cacattgttg acgcctaagc tgtacaccgc gcagccgccg
     2221 gacggcaggt tcgcggttcg ggtacagaaa ttcttggtgg tcagcgggag cgcatgaagc
     2281 atgatcttgg cgtccgcatt tcccgccgcc cggcatgtcg aagccgacat acagtagact
     2341 ggcgccagca gaagaaatag gagcaggcgg ggaaagcgcg gcagggcgga gcttcgtcgc
     2401 aacatcatcg tcggaccctc aagtagggtt gcggcaaacc ggatgcagga ccggcccccg
     2461 gggctcaacc gggtccattc aaacgtggaa tgagcctacc aaagtccatc ttgcacccgc
     2521 cacccgaaag gcgcaccatg aggtcacggg ccggccattc cgaccacgaa cccgcaaagg
     2581 ttcgacgcga tgtcgccggg tgcgaccacg ccgatgggaa gtggagccgg cccgctctcg
     2641 attcccgcaa ccacacctca cggagtcttc catgaacccg accgccacga tgaccatgcc
     2701 catcgccgac gccatggggc ggctcggcac cgagacggcc ttcgaagtgc tggcgcgcgc
     2761 ccgcgcgctg gaggcacagg ggcgggacat cgtgcacctg gagatcggtg agccggattt
     2821 cgatacgccg aagcacatca tcgcggaggc gcatgcggcg ctcgacgcgg ggtacacgca
     2881 ctatggcccc tcggccgggt tgccggagct ccgcgaggcg atcgccgcgc acgtcgtgaa
     2941 gacgcgcggc gtgccgaccg aggcggccca ggtggtggtg gtgccgggcg ccaagccggt
     3001 catgttctac ggcatgatgg ccacggtgaa cccgggggat gaagtcatct acccgaaccc
     3061 gggctttccg atctatgagt cggtcatcaa gtacctcggc gcgaagccgg tggcgctgcc
     3121 gctgcaggag tcgagggagt ttcgcttcga cctggccgac ttgaaggcgc gcatcaacga
     3181 gcggacgaag atggtcgtgg tgaacacgcc gcagaacccc accggcgggg tgctcacgaa
     3241 ggacgacatc gccggcatcg tggagctggt gcgtggacgg aacatctggg tgctgtccga
     3301 tgaaatctat tccgatctgc tctatgaagg ggaccacgtg tcgctctacg cgcaggcggg
     3361 gatgcccgag cagtcgatcc tgctcgacgg cttttccaag acctatgcca tgaccggctg
     3421 gcgcctgggc tacggcgtga tgccgaagga cctggccgtg cacgtggcgc ggctcgccac
     3481 caactgcaac tcgtgcgtca cgtcgttcgt gcagcgcgcg ggggtggcgg cgctcaccgg
     3541 cccgcgcgac gaggtcgatg ccatgaaggc cgagttccac cgccggcgcg actggatcgt
     3601 gcaggggctc aatgccattc caggcctgtc gtgcgtcagg ccgcacgggg cgttctacgt
     3661 cttcccgaac atcacgaaga ccgggctcac cagcgaggag atggccagac gctgcctcga
     3721 cgaggccggc gtggccgtgc tctccggtac cgcgttcggg gcgcatggcc agggttacct
     3781 gcgcctgtcg tacgccaatt cgcttccgaa catcgagaag gcgctcgagc gcctcgacgc
     3841 cctctgccgc tcgctcgtgg ggtaggctcg atcgacgcgc actttcccaa tccgttcggc
     3901 cgacatgatc tcttgatcgc gctcggcggc aacgcgctgc tgcccgccca cggcagcggc
     3961 accatcgacg agcagatcgc cataacaagg cgcaccatgc gggtggtggc gcgcctggcg
     4021 ctgcccggcg tgcggctggt ggtgacccac ggcaacgggc cggtggtcgg caacatcctc
     4081 gtccggaacc tcgccgccgc ggatcggatc gcgccgatgc cgctcgacgt gtgcggcgcc
     4141 gacagccagg gtgggctcgg ctacatgatc cagcagacgc tggagaacga actcttcctc
     4201 ctgggccggc atcgaccggt ggtcaccctg gtgagccagg tgcgggtgga cgggcgggat
     4261 cccgccttcg agcacgccga caagccgatc ggccccttcg tgacggcggg cgaggcggag
     4321 cggctccgcg ccgagcatgg ctgggagatg gtccaggatg cggggcgcgg ctggcggcgc
     4381 gtggtgccgt cgcccatacc cgtggagatc gtggaggagc aggccatccg cacgttgcgc
     4441 gattccggtt tcatcgtgat cgcgctcgga gggggcggcg tgcccgtgat ccgccgggac
     4501 gacggcacgc tggagggagt ggaggccgtc atcgacaagg accgcgcctc ggcggtcctg
     4561 gcccgtgacc tgcgcattcc gagcctcgtg atcgtgaccg cggtcgaccg ggtgcggctc
     4621 ggcttcggca cgcccgacga acagcctctc tccgccatga cgctggcgga ggcgcgtctc
     4681 tacctggccg agggacagtt cggcgccggc tccatggggc ccaagatcga ggccgtctgc
     4741 gacttcctcg acgcgggcgg gagagaagcc attctctgcg cgccggacca gctggccgag
     4801 gcgctggggg gcgaggccgg cacccgggtg acgcgctagc cgattttccc ccttgcggag
     4861 tcggccgcat cgtgccgcat gatgtgcgtg gtcccgtccg gattcactga cccgtccgga
     4921 agggagcgat catgttcgct cgattcattt ccctcgtggc cgtgctgtgc gttgcggccg
     4981 cacccgtttc cgccgaggtg ctcctcaacg agatgctggc cgacccggcc cgagactgga
     5041 acggcgatgg cacgaccagc agtcgggacg acgagtggac ggagatcatc aacaccggcc
     5101 ccggagtggc cgacctcacc ggcttccggc tggccggccc cgactccgtg tggcggtacg
     5161 agttcaccgg ctcgctcgca ccgggtgcgg tgaaagtggt gtatggccgg caatcgtacg
     5221 actgggaggt ggccaacgga ttccccgcgt tcggcctgcg gcttggcaac tccggggggg
     5281 aactcgtcct cttccgggtg gcgggcgcca cggtcacgca gatcgattgc tacgcctaca
     5341 ccgatcacga ggcggatgac gatcgctcga gtggtcgggg tcccgacggc agccccaact
     5401 ggatcctgct cgacggccac aatccctgga cgggaactgc cgagccgaag ggcaccggct
     5461 gccttccgac gcccggggcc aggctcacct gcgccacgcc ggtcgaggca gtcacgtggg
     5521 gccgggtgaa ggcgctgatc gatctcgatc ggtagggcaa ggcgagggag cccgcggatc
     5581 agtgcgggag cgcgcggaca ccaagaagaa acgggctcgg cgcgcatggc gccgggcccg
     5641 ttgatcttct tcagggattt ggaaccgtgg tgcggaaggg gggactcgaa cccccacgtc
     5701 gttgccaaca ctggaccctg aacccagcgc gtctaccaat tccgccactt ccgcgcccga
     5761 ttgggacgca aaacagtacc ggtgcgggtg tccaatgtca accgtgaggg cgacgtcggg
     5821 cggtgcgctc gaaaatcgcg actcggggag gaaagcgctt cccaagcatt ccggggcccg
     5881 aacgcggctg atatactccc gcgatgcatt ttctggatcc gcgtcatctc atcgagtcgt
     5941 tcggcgcttt tgcctacatc gggctcttcg ccgtggtctt cgccgagtcc ggcctcttct
     6001 tcggcttttt cctgccgggt gacagcctgc tcctcaccgc cggcctcttc gcgtacaagg
     6061 gattcctcga catcaagatc ctgctgccgc tgctcttcgt ggccaccgtg ctcggggacc
     6121 aggtcggcta ctggttcggt cgcaaggtcg gcccgccgct gttcaagcgg aacgagtcgc
     6181 tcctcttccg gccgagaaac ctggcggcgg cccggagctt ctacgaaaag cacggaggca
     6241 agacgatcat cctcgccaag ttcatgccct tcatccggac cttcgcgccg atcgtggccg
     6301 gcgccgtggc gatgccctgg cggcgcttca cgatgttcag cctgaccggc gggctcgtgt
     6361 gggccatcgg cctcacgctg gccggctacc tgctggggcg cgcgttcccg cccgaggtgc
     6421 tcgatcgcta tttcatcgtg atcgtcggcc tggtcatcgt ggtgtcggtg gcgccgtcgg
     6481 tggtgcacgt ctggaaggaa catcgcgcgc ggagcgcctg acatgacagc cggcaaggcg
     6541 ccccccggcg cggaacgggg gggtgagatc aaggtcgtct gcaccaaccg gaaggcgcgg
     6601 catctctacc atatcctcga ttcctgggag gcgggcctga tcctgacggg caccgaggtg
     6661 aagagcctga gggcgggcca cgccaccatg accgatgcct acgccgcgct ccaggggatg
     6721 gagatgatcc tccacaacct gcacatcaac ccgtacgagc aggggaaccg ctacaaccac
     6781 cagccaaccc ggccgcgcaa gctgctgctg caccgtcgcg agctgtcgaa actggtcggc
     6841 cgcaccgtgg agaaggggct cactctcgtt ccgctgcagg tctatttccg cggcggctgg
     6901 gccaaggtcg agattgccct ggcccgcggc aagaagatct acgacaagcg gcgcgacatg
     6961 gccgatcgcg agatgaagcg cgacgtcgag cgctcactca aggagcgcag ccggtgatcg
     7021 gcaagcgttt cgcggcaatc atggccgccg ccatcaccct ggcggtttgc caaacggcga
     7081 ccccagtcgc cgtcccgagc ttcgcccaga cagttcccgc accgggacgc atacacatcg
     7141 tgtacggcga cggccgtccc gaggagcagg ccgccattct cgaggccggg ccgcgcacct
     7201 ggctctcggc ccgcgacctc tcggccgtga tcggcgcgcc ccgctactgg cggagcgatc
     7261 tcaagcgcct cgtcatccgc acccccacgc acgagatggc gctggtcgtc gattcggacg
     7321 tggccatcct cgacaagacc acgccgctcc acctgccggc gccggtactc ttcgtggccg
     7381 acggcgtctg gatcccgatc gaactcctcc tcgacgccga ccggtacgat ttcggcgtga
     7441 acgggctcgg gggcgcctcc ccctggtttg accttccggt gcgctgggat gccgcggccc
     7501 gccgcctgga tgccggcacg ccgggcgccc gcctcaccgc catcacctca ccggccgacg
     7561 atccgggccg gctcgacatc ctggtcgacg gcgcgtggga atggcgactg gcggcggcgg
     7621 accgggcgcg gatggtcctc agactgatcc gcgtggggct tgccgattcg gtggcaacgc
     7681 ccgcggcgtc ggacttcatc tcggggctca gcgcgacgcc gattcccgag ggaatcgaga
     7741 tcggtttcac tccgggggcg ctggccgtcg gctaccgcgt gcaacgtctg gatcgcccgt
     7801 cgcgcctgtc ggtgctcgtc tccagtgatc ccgccgacct cgccaccgga cgcgtcaccc
     7861 cgttcgcccg gcccgagggc gccgacgttc ccgtgttcgg ccaggagacg gcccgggaag
     7921 ggggggtccg caccatcgtc ctcgacccgg ccggcggcgg gtcggccggc atccgggcca
     7981 aggggacgag cgaatcgacc gccatgctcg agctggcaca gg
//
LOCUS       DASVOD010000356         4005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_129346, whole genome shotgun sequence.
ACCESSION   DASVOD010000356 DASVOD010000000
VERSION     DASVOD010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129346"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1481)
                     /locus_tag="VF720_08250"
     CDS             complement(<1..1481)
                     /locus_tag="VF720_08250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEX7879385.1"
                     /translation="MCPDRLSFRRWLAPAAVAAAALVLSLPPSARAVERSEVPEKYTW
                     DTTDLYATPGDWEKARTDLAERTLTIARYKGRLGESPQVLNEALDTWMGLLQEMSRLS
                     SYANMTFDEDTRVSKSQERQQAAGKIYTDFGAAAAYIRPELIGIGSTKINGMMAAEPA
                     LTQYKVLLDNILRYEAHTLSSAEEKIAAMAGDMAGAGNDAYSILTDADMPYPTITLST
                     GEEVRLDASAYSRWRQAPNREDRVKVFQAFWKAHDNFKRTLGTTLYAGVKAHIFNKDV
                     HKYGSCLEAALFGDNIPVKVYHQLIADVNENLPTLHRYLKLRQRMMGLKELGYEDLYA
                     PIVSEVDMKFTPEQAQEVTLEATQVLGGEYNQALKTAFTDRWMDWMPSTGKSSGAYST
                     GVYGVHPYQLQNFNGQYDDVSTLAHEAGHSIHTYLSMKNQPFVTSDYATFVAEVASTA
                     NENFLLHTMLARTTDEDTKLFLLGSYLENMRTTLFRQTMFAEF"
     gene            complement(1658..2938)
                     /locus_tag="VF720_08255"
     CDS             complement(1658..2938)
                     /locus_tag="VF720_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX7879386.1"
                     /translation="MPPEKFSEGKGYKVRDISLAAEGRMKIDWAQSKMPVLMALREKH
                     AAARSLKGMKVTGCLHVTKETAVLVETLVAAGAQISWSGCNPLSTQDDVAAALAKAGH
                     SIYAWHGMNVEQFYWCIDRTLDMKPTLTLDDGADLIISVHTRAQKHMDTIIGGTEETT
                     TGVHRLRAMAADGKLRYPVIAVNDAETKWDFDNVYGTGQSSIDGILRATSVFLAGRNF
                     VVAGYGHCGKGVAMRAAGMGAKVIVTEVKPTMALKAHLDGFQVMKMDDAAKVGDIFIT
                     ATGMKDVIVTRHFEKLKDGAIICNTGHYDCEINLGELGKLTKKVTTIRPDCEEYTLKN
                     GRKIFVLAQGRLVNLAAAEGHPSEVMDMSFANQFLSLVRLAKEGRKMKPMVYDIPESQ
                     DQDLARLKLDTLGMKLDKLTKEQIAYASDYLSGT"
     gene            complement(2941..>4005)
                     /locus_tag="VF720_08260"
     CDS             complement(2941..>4005)
                     /locus_tag="VF720_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7879387.1"
                     /translation="INRTDADPARLFRTLGDPSRLRLLRLLAWEELNVAEICRVVGLP
                     QPTVSRQLALLKESGLVADRKSGPWVFHRLAVDAAPAMTRNVIGAVRPTLDEASPEAD
                     RDRTRLDRVLRERQETARLYYASGTESAGTRQSRFGDATAWRTLAGLLPRQGTIVDLG
                     TGAGDLLPQLSPRAGRVVALDFSEAMLSHARARAKALRLPNVAFVRGELEAVPLPNAS
                     ADGVVASLVLHHAARPEAAIREMARLLAPGGRAVVVDFLPHREDWLKDEEGDVWPGFD
                     PKALHGWFARAGFSDILIEEGPAPVSSGRRDRPGSDRRLKNLRLQWVEAVRAGEPAAP
                     ARTPRQPNSKRVSRTTTNRR"
BASE COUNT          599 a         1284 c         1377 g          745 t
ORIGIN      
        1 tcgaactcgg cgaacatcgt ctggcggaag agcgtggtgc gcatgttctc gaggtaactg
       61 ccgaggagga agagcttggt gtcctcgtcc gtcgtgcggg ccagcatggt gtggaggagg
      121 aagttctcgt tggccgtgga cgccacttcg gccacgaacg tggcgtagtc gctggtcacg
      181 aaaggctggt tcttcatcga caggtacgtg tggatcgagt ggccggcctc gtgggccagc
      241 gtgctcacgt cgtcgtactg gccgttgaag ttctgcagct ggtacgggtg cacgccgtac
      301 acgccggtgg agtaggcgcc cgagctcttg ccggtggacg gcatccaatc catccagcgg
      361 tcggtgaagg ccgtcttgag cgcctggttg tactccccgc ccagcacctg cgtggcttcg
      421 agcgtcactt cctgggcctg ttccggcgtg aacttcatgt ccacctcgga cacgatcggg
      481 gcatagagat cctcataccc gagttccttg agccccatca tccgctggcg cagtttcagg
      541 taacgatgga gcgtcggcag gttctcgttc acgtcggcga tgagctggtg gtacacctta
      601 accgggatgt tgtcgccaaa cagggctgcc tcgaggcagc tcccgtactt gtgcacgtcc
      661 ttgttgaaga tgtgcgcctt cacgcccgca tacaacgtcg tgcccaaggt ccgcttgaag
      721 ttgtcgtggg ccttccagaa ggcctggaac acctttaccc ggtcctcgcg attgggcgcc
      781 tggcgccagc gggagtaggc cgatgcgtcg agtcgcactt cctcgccggt cgagagcgtg
      841 atggtcgggt agggcatgtc cgcgtccgtc aggatcgagt aggcgtcgtt gccggcgccg
      901 gccatgtcac cggccatggc ggcgattttc tcctcggcgg acgagagcgt atgggcctcg
      961 tagcggagga tgttgtcgag gagcaccttg tactgggtga gcgcgggctc ggctgccatc
     1021 atgccgttga tcttcgtcga accgataccg atgagctccg gccggatgta cgccgccgct
     1081 gcgccgaagt cggtgtagat cttcccggcc gcctgctggc gttcctggct cttcgagacg
     1141 cgcgtatcct cgtcgaacgt catgttggcg tacgacgaca ggcgtgacat ctcctggagc
     1201 agccccatcc aggtatccag cgcctcgttg agcacctgcg gcgattcgcc gagacgtccc
     1261 ttgtacctgg cgatggtcag cgtccgctcg gccaggtcgg tgcgggcctt ttcccagtcg
     1321 cccggggtgg cgtaaaggtc ggtcgtgtcc cacgtgtact tctcgggcac ctcggaacgc
     1381 tcgacggcgc gggcgctcgg cggcagggac aggaccaggg cggcggcggc cacggcggcc
     1441 ggcgcgagcc aacgtcggaa cgacagtcgg tcaggacaca tggacggatt tctccaggag
     1501 ggatggcgag gcatggccgg tcggcgacgc cgcaaaacag ggggacctgc gatcccgggg
     1561 caaaaggatc ggcggcgggg cgaattctaa ccgcgcccgc cgccgtcatc cagtcagcga
     1621 gtaaacgagt tgtaaccgcc ggcggaccgg cgcgccctta cgtcccggag aggtagtccg
     1681 acgcgtaggc gatctgttcc ttggtgagct tgtcgagttt catcccgagc gtgtcgagct
     1741 tgaggcgggc caggtcctgg tcctgcgact cggggatgtc gtacaccatc ggcttcatct
     1801 tccgcccttc cttcgcgagg cgcacgaggg agaggaactg gttggcgaat gacatgtcca
     1861 tgacctcgga cgggtgcccc tcggccgccg ccaaattcac gaggcgcccc tgcgccagca
     1921 cgaagatctt cctgccgttc ttcagggtgt attcctcgca atcggggcgg atcgtcgtca
     1981 ccttcttcgt gagcttgccc agttctccca ggttgatctc acagtcgtag tgccccgtgt
     2041 tgcagatgat cgcgccatcc ttcagcttct cgaagtgacg cgtcacgatg acgtccttca
     2101 ttccggtggc cgtgatgaag atgtcgccca ccttcgccgc gtcgtccatc ttcatcacct
     2161 ggaagccgtc gaggtgcgcc ttgagcgcca tcgtcggctt cacctcggtg acgatgacct
     2221 tggcgcccat gccggccgcg cgcatggcca cgcccttgcc gcagtggccg tagccggcca
     2281 ccacgaagtt gcgccccgcc aggaacacgc tggtggcgcg caggatgccg tcgatcgacg
     2341 actggccggt gccgtacacg ttgtcgaagt cccacttcgt ctcggcgtcg ttgaccgcga
     2401 tgaccgggta acgaagcttg ccgtcggccg ccatggcgcg gaggcgatgc acaccggtgg
     2461 tcgtttcctc ggtgccgccg atgatcgtgt ccatgtgctt ctgggcgcgc gtgtgcaccg
     2521 agatgatgag gtcggcgccg tcatccagcg tgagcgtcgg cttcatgtcg agcgtgcggt
     2581 cgatgcacca gtagaactgc tcgacgttca tgccgtgcca ggcgtagatg gagtggccgg
     2641 ccttggccag cgccgccgcc acgtcgtcct gagtggaaag gggattgcag cccgaccacg
     2701 aaatctgggc accggcggcc acaagggttt cgaccagcac ggccgtctcc ttggtgacgt
     2761 gcaggcagcc ggtcaccttc atacccttca ggctcctggc cgccgcgtgc ttctcgcgca
     2821 gcgccatgag caccggcatc ttgctctggg cccagtcgat cttcatccgg ccttcggccg
     2881 ccaggctgat gtcgcggacc ttgtaaccct tgccttcgct gaacttctcg gggggcatgt
     2941 tcatctcctg ttggtggtgg tgcgggaaac gcgtttcgaa ttcggttggc ggggggtgcg
     3001 tgcgggcgcc gccggttccc cggcacgcac ggcttccacc cactgcagtc tcaggttctt
     3061 gaggcgccgg tcggaaccgg ggcgatcacg gcggccggag ctcaccggcg ccggtccttc
     3121 ctcgatcagg atgtcgctga agccggcccg ggcgaaccag ccgtgcaggg ccttgggatc
     3181 gaagccgggc cagacatccc cctcttcgtc cttgagccag tcctcgcggt gcggcaggaa
     3241 atcgacgacg acggcccggc ctcccggcgc gaggaggcgc gccatctcgc ggatcgcggc
     3301 ctcggggcgc gcggcgtggt gcaggacgag gctcgccacc acgccatcgg cggaggcgtt
     3361 tggcaggggc acggcctcca gttcgccacg cacgaaggcc acgttgggga ggcgaagcgc
     3421 cttcgcacgg gcccgggcgt gggagagcat ggcctccgag aaatcgagcg ccaccacccg
     3481 gccggcgcgc ggggagagct gtggcaggag gtcgccggca ccggtgccga ggtcgacgat
     3541 cgttccctgg cgggggagga ggccggccag cgtgcgccag gctgtggcgt cgccgaagcg
     3601 ggactgccgg gtgccggccg actccgtgcc gctcgcgtag taaagccggg ccgtttcctg
     3661 ccgctcgcgg agcacgcggt cgagccgggt gcggtcacga tcggcctcgg ggctggcttc
     3721 atcgagtgtc ggtcgcactg ccccgatgac attgcgggtc atggccgggg cggcatcgac
     3781 cgcgaggcgg tgaaataccc agggccctga tttccggtcg gcgaccagtc ccgactcctt
     3841 gagcagcgcc agctgccgcg acaccgtcgg ctgcggcaac cccaccacgc ggcagatctc
     3901 ggccacgttc agttcctccc aggcgagcag ccggagaagc cgcagccggg acgggtcacc
     3961 cagggtgcgg aagaggcggg ccggatcggc atcggttcgg tttat
//
LOCUS       DASVOD010000357         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_911304, whole genome shotgun sequence.
ACCESSION   DASVOD010000357 DASVOD010000000
VERSION     DASVOD010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911304"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF720_08265"
     CDS             <1..369
                     /locus_tag="VF720_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879388.1"
                     /translation="GRPVEAMVHLDEARILEAENAPAEALRGWASLVAGKPDEALAHA
                     RQAKAWGEWCDLAWIVEAHVLKAGGDAAGAARAVEAVQTRVRENAPPSYLYLPKLATW
                     QSIHELPAVERELLEQLTRP"
     gene            complement(482..991)
                     /locus_tag="VF720_08270"
     CDS             complement(482..991)
                     /locus_tag="VF720_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879389.1"
                     /translation="MTRFINTPTGLVRNITALSTLTVLSLLAIVTLVGCGTDVSTAPE
                     FVNIESDDGGYQPGEVPLPSRKGAVEAPIGLTLQQISSTGALLTWNEPSTGLTALIDL
                     NGVRIAEVDASNGNFTDNLAKSPGMYHYTVCFQNSVKRMSQMKGVDGEVKAVPDGSDR
                     RDDRMEDAH"
     gene            complement(1786..>3162)
                     /locus_tag="VF720_08275"
     CDS             complement(1786..>3162)
                     /locus_tag="VF720_08275"
                     /inference="COORDINATES: protein motif:HMM:NF039629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HEX7879390.1"
                     /translation="RKDGVESFEDLFRVDASWRRWTIGARFHAIEGSEAPGSEEIDQR
                     YLQYVDDHLELLGGNFYETWGRGLVLRAYETRTATVGRLDRSVALDRDIDGFRARARY
                     GRLFGTALSGSPLLAPLSGEPASDGAGVRLDTMRGARAGILAPGGLTATGGYLRINTI
                     DPGAPDPESAERIRDEYLTGEADFVRGPVVAHLSGARRHADELLLPEDGRAWYGSASA
                     SVSTFGASFEFKDYERFVSPYNEPPTLVKTQSWTLLNRGTHLSNPDDEVGQQAALEWS
                     PGPEDGVSLNHSRADNHDRDEIFEYRQWTLEARLRTGADDSGPRFKAIADWERDRIKG
                     ITDRWTAGGDMEIGFGDIRSIATVFEHQVTDFEFADQQRLSLLTLEYQEAPWLTVAFQ
                     GEKAAEPEEGRDTWASATVNLSWSGRHNLNIFVGRRPTGYLCTGGYCFLAPAFDGIAL
                     RLISLFGD"
BASE COUNT          518 a          980 c         1094 g          570 t
ORIGIN      
        1 ggccggccgg tcgaggcgat ggtgcacctc gacgaggcca gaatcctcga agcggaaaac
       61 gcgcccgccg aggcgctgcg tgggtgggca tccctcgtgg ccgggaagcc ggatgaggcg
      121 ctggcccacg cccgccaggc aaaggcctgg ggcgagtggt gcgacctggc gtggatcgtg
      181 gaagcccatg tcctcaaggc cggcggtgac gcggccggtg ccgcgcgggc cgtggaagcg
      241 gtgcagaccc gcgtgcgcga gaacgcgccg ccctcctacc tctatcttcc gaagctggcc
      301 acctggcaat cgatccatga actgccggcc gtcgagcgcg aactgctgga acagctgacg
      361 cgaccataag ccggcggggc gacccttgtg gggccgcccc gcctccgaca tcgcccggcg
      421 cgggcctctc ctgacccttt cgggtcccct caccggtcga tgaagactgg tgctggctgg
      481 atcagtgggc gtcttccatc cggtcgtcgc ggcggtcgct gccgtcgggc acggccttca
      541 cttcgccgtc gacgcccttc atctggctca tccgcttcac gctgttctgg aagcagaccg
      601 tgtagtggta catgccgggg ctcttggcca ggttgtccgt gaagttgccg ttcgaggcat
      661 ccacctcggc gatgcgcacg ccgttcaggt cgatcagggc cgtgaggccg gtgctcggtt
      721 cgttccaggt gaggagcgcg ccggtggacg agatctgctg gagcgtcagt ccgatcggag
      781 cctcgacagc tcccttccgt gaggggagcg ggacttcccc cggctggtat ccgccatcat
      841 cgctctcgat gttgacgaat tccggcgccg tggacacatc ggtgccgcaa ccgacgagcg
      901 tgacgatggc gaggagcgac aggacggtaa gggtcgacag ggcggtgatg ttccggacga
      961 ggccggtcgg ggtgttgatg aagcgggtca tgacgtcctc cttgagaaga ttccgttggc
     1021 ttcggctgcg ggtcttcgtt gcgttgcccg cgtcggatga tgcggacggg aacaggtcaa
     1081 agcagcgatt atgccaatgg ccggggaggg cggcgccatg aaggtaattc gatgtcttta
     1141 ttgaagatat ggattagttt ccgatggggc ctggcgggcc gcagcgcggc caacaataag
     1201 ctttgttaac gcgaatggcg gtggttggcg cggggggttt acacgttaac aggtggcctg
     1261 attcgcgaac agatcaattg cggcattgac cgcgtacctg tccccgagca ggtggcgcga
     1321 ccagcgttcc caggcgaccg cgttgggact ctcggagagc acctgttccg gaacaagtaa
     1381 gcgtgttggc accaggcgac cgcgttggga ttcactgaga gcacctgttc cggaacaggt
     1441 aagagtgttg gcacctgccg accacgtcat gactttcgga gagcacctgt tccggaacag
     1501 gtaagcgtgt cggcaccagg cgaccacgtc gtgactttcg gagagcacct gttccggaac
     1561 aggtaagcgt gttggcacct gccgaccacg tcgtgactcc cggagagaac ctattccgga
     1621 acaggtaagc gtgttggcac ctgccgaccg cgttgggatt cactgaggga acctgttccg
     1681 gaacaggtaa gcagcatcca cccgctggcg gcggtgtcag gctcggcggc ggcgtcacgc
     1741 tctctaagag agcgcgccgc gagctccacg cgaatcgcgc gtctcctaat ccccgaacag
     1801 gctgatcaaa cgtaacgcga tgccgtcgaa tgcgggggcc aggaagcagt agcccccggt
     1861 gcagaggtag ccggtggggc gtcggccgac gaagatgttc aggttgtggc ggccgctcca
     1921 ggagaggtta acggtggcgg aggcccaggt gtcgcgcccc tcttcgggct cggcggcctt
     1981 ttctccctgg aacgccaccg tgagccaggg cgcttcctgg tattcgagcg tcaggagcga
     2041 cagtcgctgc tggtcggcga actcgaagtc ggtgacctga tgttcgaata cggtcgcgat
     2101 gcttcggatg tcgccaaatc caatctccat gtcgccgccg gcggtccagc ggtcggtgat
     2161 ccccttgatg cggtcccgct cccagtccgc gatggccttg aagcgcgggc ccgagtcgtc
     2221 cgctccggtg cgcagccggg cctcgagcgt ccactgccgg tattcgaaga tctcgtccct
     2281 gtcgtggttg tcggcgcggc tgtgattcag cgacacaccg tcctcaggtc ccggggacca
     2341 ctcgagcgcc gcctgctggc cgacttcgtc atccgggttg ctgagatggg tgccgcggtt
     2401 cagcaatgtc caactctgtg ttttcaccag tgttgggggc tcgttgtagg gactcacgaa
     2461 gcgctcgtaa tccttgaatt cgaaagacgc tccaaacgtc gacaccgagg cgctggccga
     2521 gccgtaccaa gcgcggccgt cttccggcag caggagttcg tcggcgtgtc ggcgggcgcc
     2581 gctcaggtgg gccacgaccg ggccgcgcac aaagtcggcc tcgccggtca ggtattcgtc
     2641 gcggatccgt tcggcggatt ccggatcggg cgcaccgggg tctatcgtgt tgatgcgcag
     2701 gtagccgccg gtggccgtga gtcccccagg cgccaggatg ccggcgcggg cgccgcgcat
     2761 ggtgtcgagg cggacgccgg cgccgtccga ggccggctcg ccagagagag gcgccagcag
     2821 cgggcttcct gaaagcgccg ttccgaagag gcggccgtaa cgcgcgcgtg cccggaagcc
     2881 gtcgatgtcg cggtcgagcg caacggatcg atcgaggcgc cccactgtgg ccgtgcgggt
     2941 ttcgtaggcg cgaagcacga ggccccggcc ccacgtttca tagaaattgc cgcccaggag
     3001 ttccaggtgg tcatcgacat actgcaggta tcgctggtcg atctcctcgg aaccgggcgc
     3061 ttccgatccc tcgatcgcgt gaaaacgcgc gccgatcgtc cagcgcctcc aggaggcatc
     3121 cacgcggaag aggtcctcga acgactcaac tccatccttg cg
//
LOCUS       DASVOD010000358         2709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_77446, whole genome shotgun sequence.
ACCESSION   DASVOD010000358 DASVOD010000000
VERSION     DASVOD010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77446"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1628)
                     /locus_tag="VF720_08280"
     CDS             complement(<1..1628)
                     /locus_tag="VF720_08280"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX7879391.1"
                     /translation="MRNHLRRPALVLVPALLLVFTWGATAGAKSYRHPSVQQTVTVLP
                     DGTLDILDLRAYEFDGEFHNAFVPMIPRDGGSATLLEVRATDDGGPVRNVRVEENTIR
                     WQYDARNETRLFAIHYQLTGEIDRATDAALLDRLLVEEEHAIIDQYGLTLRVAGPAPL
                     FKVFLITERGRVGEMDVNAETGSASVTLQSLSADEWVRARVLFDPALVPALAQGSQAK
                     YEEWLRETAVETQSYRGGVRDALRARGGASRPPRAPLPTALAFLLWGLSGAFSWWAFR
                     TYRAHGIEPEVQQVGQYFREPAETIPPGAVPYVMDQFSPGIGGASAAFGATLLDFARR
                     GYLRLEEREKEKFLGLGGGREVDFILDHHPPAGELSDFEEEVWRLLEQARGDDNRMKP
                     SELRKFFKSNTLWMTRWVEKPRAWYERAKEPLLVGNHGGWMFLLIGGGILLMAGSILL
                     GAFSNNPVVFLSGILAGVTAGLFGVICGAGIPKWRSGPLLRARQWQAYRRFLSDFSAM
                     KDAPAEHYRMWDYHFIYATALGVSKEYLAGLKRL"
     gene            complement(1681..>2709)
                     /gene="ispG"
                     /locus_tag="VF720_08285"
                     /gene_synonym="gcpE"
     CDS             complement(1681..>2709)
                     /gene="ispG"
                     /locus_tag="VF720_08285"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEX7879392.1"
                     /translation="IAARLADRGVSVPLIGDFHYNGHQLLRDFPACAAALAKYRINPG
                     NVGVGKRHDENFQSMIRVAVEHGKPVRIGVNWGSLDQQLLTAMMDANARLPEPKDARE
                     VMRDAIVESALRSAELAEATGLPHDRILLSAKVSGVPDLIAVYRRLAARCDYPLHLGL
                     TEAGLSTKGVVATTAGLSVLLADGIGDTIRASLTPLPGGDRAEEVRVCQQVLQSMGLR
                     AFTPQVTACPGCGRTTSTFFQEMADQIQTFLANRMPAWRGQYAGVEELSVAVMGCVVN
                     GPGESKHAHIGISLPGTGEDPKAPVYVDGRLHVTLKGPTIVAEFIALLEDYVSRRYGG
                     DPPASGRA"
BASE COUNT          447 a          914 c          924 g          424 t
ORIGIN      
        1 atgagccgct tgagcccggc aaggtactcc ttcgacacgc caagcgccgt ggcgtagatg
       61 aagtggtagt cccacatcct gtagtgctcg gccggcgcgt ccttcatcgc ggaaaaatcg
      121 gagaggaacc ggcgatacgc ctgccactgc cgcgcccgga gcaagggccc ggaacgccac
      181 ttggggatgc ccgccccgca gatcaccccg aagagccccg ccgtcacgcc cgccaggatg
      241 cccgacagga agacgaccgg gttgttgctg aacgcgccca gcaggatcga gccggccatc
      301 agcaggatgc cgccgccgat caggaggaac atccaccccc cgtggtttcc caccaggagc
      361 ggttctttcg cgcgctcgta ccaggcgcgc ggcttctcca cccatcgcgt catccagagc
      421 gtgttgctct tgaagaattt ccgcagttcc gacggcttca tccggttgtc atcgccccgg
      481 gcctgctcca ggaggcgcca cacctcctcc tcgaagtcgg agagctcccc cgccggcgga
      541 tggtggtcca ggatgaagtc cacttcgcgc ccaccgccga gaccgaggaa cttctccttc
      601 tccctctctt ccaacctcag gtaaccgcgc cgggcgaaat cgagcagcgt ggcaccgaac
      661 gccgccgagg cgccgccgat tccgggcgag aactggtcca tgacataagg cacggccccc
      721 ggcgggatcg tctcggccgg ctcgcggaag tattgcccca cctgctgcac ttcgggctcg
      781 atgccatggg cccgataggt gcgaaaagcc caccacgaga aggctcccga caatccccac
      841 aggaggaacg ccagggccgt ggggagcggc gcacgcggcg ggcgggaggc gcctccccgg
      901 gcgcggagcg cgtcacgcac gcctccccgg tagctctggg tttcgacggc cgtctcgcgc
      961 agccattcct cgtactttgc ctgactcccc tgcgccagcg ccgggaccag ggccggatcg
     1021 aacagcacgc gggcgcgcac ccactcgtcg gccgagagcg actggagcgt gacggaggcc
     1081 gacccagttt ccgcgttcac gtccatctcg ccgacgcgac cccgctcggt gatgagaaac
     1141 accttgaaga gtggggccgg ccccgccacg cgcagcgtga ggccgtattg atcgatgatg
     1201 gcgtgctctt cctcaacgag gagacgatcg agcagcgccg cgtcggtggc ccgatcgatc
     1261 tcgcccgtca gctggtagtg gatggcgaag agacgggttt cgttccgggc gtcgtactgc
     1321 cacctgatcg tgttctcttc cacgcgcacg ttgcgaacgg ggccaccgtc gtcggtggcc
     1381 cggacctcga ggagggtggc cgacccacca tcccgcggga tcatcggcac gaaggcgttg
     1441 tggaactcac cgtcgaattc gtacgcgcgc aggtcgagga tgtcgagcgt gccgtcgggg
     1501 agcacggtca ccgtctgctg cacggaggga tggcggtacg acttggcccc ggcggtggct
     1561 ccccaggtga aaacgaggag gagggccggg acgaggacga gcgcggggcg gcgtaggtgg
     1621 ttcctcacgg gcgtacccta cgctgctgcc gcggggaggt tcaactccag gtcggaattc
     1681 tcaggcgcgg ccggacgccg gagggtcgcc gccgtatcgc cggctcacgt agtcctcaag
     1741 gagggcgatg aactccgcca cgatcgtcgg gcccttgagc gtgacgtgca gccgtccatc
     1801 cacgtacacc ggcgccttcg ggtcctctcc ggtaccggga agggagatgc cgatgtgggc
     1861 gtgcttcgat tcgcccggcc cgttcacgac gcaccccatc acggccaccg agagctcttc
     1921 cacaccggcg tactggccgc gccaggcggg catgcgattg gcgaggaagg tctggatctg
     1981 atcggccatc tcctggaaga aggtgctggt ggtccttccg catccggggc atgccgtcac
     2041 ctgaggagtg aaggcgcgca accccatcga ctggaggacc tgctggcaga cccgcacctc
     2101 ctcggcccgg tcgccgccgg ggagcggcgt aaggctggcc cgaatggtgt cgccgatgcc
     2161 gtcggccaac aggaccgaga ggccggcggt ggtggccacg acacccttgg tggaaaggcc
     2221 ggcttcggtg aggcccaggt ggagggggta atcacaccgg gccgccagcc ggcgatacac
     2281 ggcgatcagg tcgggcacgc cgctgacctt ggcggaaagc aggatgcggt cgtgcggaag
     2341 accggtggcc tcggccagtt cggccgagcg gagcgcgctc tccacgatag cgtcgcgcat
     2401 gacctcgcgt gcatccttcg gttcgggaag tctggcgttg gcgtccatca tcgccgtgag
     2461 aagttgctgg tcgaggcttc cccagttgac gccgatccgc accggctttc cgtgctccac
     2521 cgccacgcgg atcatcgact ggaaattctc gtcgtgacgc ttgccgacgc cgacgttgcc
     2581 ggggttgatg cggtacttgg cgagggcggc ggcgcaggcc gggaaatccc tgaggagctg
     2641 gtgaccgttg tagtggaagt cgccgatgag cggtaccgac acgccccggt cggccagccg
     2701 cgccgcgat
//
LOCUS       DASVOD010000359        10445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_442750, whole genome shotgun sequence.
ACCESSION   DASVOD010000359 DASVOD010000000
VERSION     DASVOD010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442750"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF720_08290"
     CDS             complement(<1..488)
                     /locus_tag="VF720_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879393.1"
                     /translation="MIHMNVGRTIATLTGLTLLVLTGCQTASKTDTGQTPPPAQSTPP
                     PDQAMNQPPAEPPPVTPGEPATTIPAETTPKPTPKPKPRPPAPKPVEPTPAPAPVVLT
                     VPAGTSIRASLNNELQSDKTAAGTPITLSVIDAVTVNGTVVIPAGATVSGEVVSAKAS
                     GK"
     gene            complement(521..2143)
                     /locus_tag="VF720_08295"
     CDS             complement(521..2143)
                     /locus_tag="VF720_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX7879394.1"
                     /translation="MKIAAIDVGSNSIHLLIARVLPDHGYEIIHKDKAMVRLGSRAFA
                     RRELTAATQRRAFVALERFAEVIRRFEVDVVVGVATSAVREASNGTAFLREVRRRTGL
                     KIDRIAGAEEARLVAVAVAGLPPFGRGRHLVVDIGGGSTELAVLNDEEPQVIDSLRMG
                     AVRLADEVRVKDRPGKKGIREYMAAAREGMGAAHRRLRDQAFDEVAGTSGTIVCLGML
                     AARRAGRTLSRGETFSVSREELTRELHYLATLALDERRDYMAEQSPRADIIVPGGAIL
                     MTVLEGVANDRVHIPDRSIRDGMILDHIRQRMEATALDHVKGLARIAGGGKGDRYDPH
                     TVRERNLVSFAMRYHYEAHHAHQVMALASQLFDETTELHGLGNEEKFYLEAAALLHDI
                     GQYIAYSQHHKHSLYLILNGSLSGFSEREIAVIGNVARYHRKGFPQLSHPEYAALAPE
                     DRKLVVTLAALLRVADALDHGHLNSVNRIRVEVTRRKAVLNLTAGRDCANEMERAARK
                     ADLFEETFGRRVAFKARRSRSDAKGSKSEAVA"
     gene            complement(2187..3692)
                     /gene="moeB"
                     /locus_tag="VF720_08300"
     CDS             complement(2187..3692)
                     /gene="moeB"
                     /locus_tag="VF720_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013051199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX7879395.1"
                     /translation="MPTPIHIPTPLRPYAGGVDRVSVDAVTVDEALARLTATHPGLKK
                     HLFNDEGRLRSFVNVYVNDEDIRHLSGGATPIKPGDELTIVPSIAGGAAPTPAAADPR
                     STEVPTSGRLADIPARAAAVTLSNDEIHRYSRHLIMPEVTMAGQKKLKAASVLMIGAG
                     GLGSPLGLYLTAAGVGRLGIVDFDVVDATNLQRQIVHGTSSLGKPKLESAKARLGDLN
                     PNIRIDTYEAALTSENALELFREYDIVVDGTDNFPTRYLVNDACVLLEKPNVYGSIFR
                     FDGQASVFWAEKGPCYRCLYPEPPPPGLVPSCAEGGVLGILPGIIGVIQATETVKLIL
                     GAGTSLVGRLLLFDALRMSFRELKLKKNPDCPICGPNRTIHELIDYQQFCGITPHEAE
                     TGEAEFEIAPAELKARLDRGDKLTIIDVREPHEYEINRIQGSRLIPLGQVPERMHELS
                     SADDIVLHCKAGLRSQKALAILRGAGFSRLKNLTGGIDRWAREVDPSLPQY"
     gene            complement(3695..4174)
                     /locus_tag="VF720_08305"
     CDS             complement(3695..4174)
                     /locus_tag="VF720_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEX7879396.1"
                     /translation="MSGTSSPRPRTDRAGGLLIRPSDLEAIRRHGEEAFPHECCGLLL
                     GKVEPDGRTKRVTTVEPLANEREESRHNRFLITPETFLAADRSARRRGIDILGFYHSH
                     PNAPAVPSEFDREHAWPFYSYIIVSVVDGGARELDSWTLAEDRTRFDAEPVVPARED"
     gene            complement(4171..5133)
                     /locus_tag="VF720_08310"
     CDS             complement(4171..5133)
                     /locus_tag="VF720_08310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEX7879397.1"
                     /translation="MRLIDDLARHEPRLARHAHELLAQVGSTPLLRLNRVTRDLPADV
                     QVFGKAEWFNPGGSVKDRPALRIVLEALRVGRLKPDGTLLDATSGNTGIGYAMVGAVL
                     GIRVTLCLPARVSPERLATLRAYGAELVLTDPLEGTDGAIREAARLSRAEPGRFFLAD
                     QYTNPANWRAHLDTTGREIWEQTAGRVTHFVAALGTSGTFMGVGRRLKREGPDVHLTS
                     FQPDSPFHGLDGVKHMPSAIVPGIFDPTLADRNEEVGTEEAYAMTRRLAREEGLRVGP
                     SSGAAVVVALRVARRLSRGTVVTVFPDGGDRYLGERFWTEEDES"
     gene            5261..6106
                     /locus_tag="VF720_08315"
     CDS             5261..6106
                     /locus_tag="VF720_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7879398.1"
                     /translation="MLTEEPFEIEGEVGGSIRGDVRWGPDSGPTIVIAHGFKGFKDWG
                     HFPFVSRQFAGAGFRVVSFNFSGSGIGEKPTEFTELDRFRHNTLSREVHELDRLITSA
                     AAGRLPGGATIGRVGLLGHSRGAIATTVVADRRSDIGALVTWAGVGVLRERYPDDVRR
                     AWRETGELPVVNARTGQSMPIGLEALDDLEQHLDDYSPDVISAGLDVPHLLIHGDADA
                     GVPIEESRAVSRASHGKSRLIEIPGADHTFGSVHPFQGEGPALMEVIEHTLAFFGEHL
                     SKQAR"
     gene            6392..7690
                     /gene="larA"
                     /locus_tag="VF720_08320"
     CDS             6392..7690
                     /gene="larA"
                     /locus_tag="VF720_08320"
                     /inference="COORDINATES: protein motif:HMM:NF033504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HEX7879399.1"
                     /translation="MKRHSPIDFPYGRDRITWVPDPALDVTLVEPREPPPAQDPGRLI
                     REALENPLGSPLLAELSRGAKSATIVVTDATRPCPDRLLVTPILETLAAAGIPDTAIT
                     LLVAIGMHRPSTPEEKIEKLGDAIVSRYRVVDSAPLDPRLQVDFGTSRDGVPITIDRR
                     AADADLLIATGIVEPHQYAGFSGGWKTVAIGTAGAATITALHGMHYLEHPRVRLGMTD
                     DNPFLDTARWVGEKAGLRFVVNVALDPNGVIRRVRAGHPETVHRDMSAWVRGEAIVPV
                     DGQFPIVIGAAGHPKDSNLYQATRIPTYLCYARRPVVAPGGVILVPAACPEGAGQGAG
                     ERLFEEMLGQGGSPDEIIERARREGYPAGGQRAVMVAWAMKNARLAFVGTIDPELPRR
                     LGMLSFRTLEEGVEWAVRDTGSERILAVPHALMTLPEKCE"
     gene            complement(7802..8269)
                     /locus_tag="VF720_08325"
     CDS             complement(7802..8269)
                     /locus_tag="VF720_08325"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX7879400.1"
                     /translation="MAMFGSAKKDGGAPADTRINTVIGEGTSLTGTLRVEGSILVHGE
                     FEGTLTATESAIVGKTGNVRAGITARDILVAGKVRGKLVAKDRVELQTGARLEGDVLA
                     KSFMIEEGVFFQGNCSMGDDVVIEEASSAHGSGGSAAADPATTQRGLGLLGQR"
     gene            complement(8293..9216)
                     /locus_tag="VF720_08330"
     CDS             complement(8293..9216)
                     /locus_tag="VF720_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX7879401.1"
                     /translation="MTRKALGRGLGALIPEAPTPTSSAGPSDRPAAVAVATPAGTPID
                     LPIASISGNPYQPRHHFDAERLRELSESIRMSGVLQPVIVRHAPDGRGYQLIAGERRL
                     KAAELAGRDSVPAVVRMASRKEMLEFALIENIQREDLDPIEEAHAYQRMASEFDLTQE
                     QIATRVGKDRTTVANSLRLLNLPEAVQEMLSAGKLSTGHARCLLAIESATQQEAMASD
                     IVRRGFSVRQVEEMTAGHRQTRPRGTGRRGRVAPALADWEDRLRRVYGTQVRILGGTA
                     RGRVEISYFSESDLERVLEIAGLIGERTPRD"
     gene            complement(9194..9970)
                     /locus_tag="VF720_08335"
     CDS             complement(9194..9970)
                     /locus_tag="VF720_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX7879402.1"
                     /translation="MARVVAVANQKGGVGKTTTTVNLAAALALAGRRVLLVDIDPQGN
                     ATSALGSNGNELEETIYEVLVNELPLESVRQPADIATLHVVPAAQRLIGAEIELVPVP
                     ERERVLRRRLEAVRDAYDYILIDCPPSLGLLTINAMTAADSVLIPLQTEYYALEGLSQ
                     LLNAIRLIQRGLNPTLEIEGILLTMFDARLNLSHQVSEEARKFFPGKVYQSVIPRNVR
                     ISEAPSFGQPVVTYDPKSAGAESYFNLAKEVLNHDTQSTG"
     gene            complement(10220..>10445)
                     /locus_tag="VF720_08340"
     CDS             complement(10220..>10445)
                     /locus_tag="VF720_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879403.1"
                     /translation="GELGAGLATARAVDRLEQVWSWASGLLRSGGELAMYRAPGEGGE
                     SLAELDPAPAAVRRVAMPGQPRELLFLTLG"
BASE COUNT         1643 a         3521 c         3459 g         1822 t
ORIGIN      
        1 ctcttgccgg aggccttggc cgaaacgacc tctccggaca cggtggcgcc ggcgggaatc
       61 acgaccgtcc cgttcaccgt cacggcgtcg atcaccgaca gcgtgatcgg cgttccggcg
      121 gcggtcttgt cgctctgaag ctcgttgttc agagaggcgc ggatggaagt gccggccggc
      181 accgtcagca cgacgggtgc gggcgcgggt gtcggctcga cgggtttcgg ggccggcggc
      241 ctgggcttcg gcttgggagt cggcttcggc gtcgtctcgg ccgggatcgt cgtggccggt
      301 tcacccggtg tgaccggtgg cggctccgcg ggcggctggt tcatcgcctg gtcgggcggc
      361 ggtgtgctct gcgctggggg cggcgtctgc cccgtgtcgg tcttgctggc cgtctggcaa
      421 ccggtcaaca cgagcagcgt caacccggtc agggtggcga tcgtgcgtcc gacattcatg
      481 tgaatcatgg aagcctcctt gaatgtgtcg aatctattcg tcaggccacg gcctcggact
      541 tcgatccttt ggcatcgctg cggctgcggc gggccttgaa cgccacgcgc cgaccgaagg
      601 tttcctcgaa gagatcggcc ttgcgggccg cccgctccat ctcgttggca caatcgcggc
      661 ccgccgtaag gttcagcacg gcctttcgcc gcgtgacctc cacccggatc cggttcacgg
      721 aattcaggtg accatggtcc aaggcgtccg ccacacggag gagcgcggcg agcgtcacca
      781 ccagcttccg atcctccggg gccagcgccg cgtattcggg atgcgagagc tgcggaaatc
      841 ccttgcgatg gtatcgggcc acgttgccga tcacggcgat ctcccgctcg ctgaacccgg
      901 aaaggctgcc gttcaggatc aggtagaggg agtgcttgtg gtgctgactg taggcgatgt
      961 actggccgat gtcgtgcagc agggccgccg cctcgaggta aaacttctcc tcgttcccga
     1021 ggccgtgcag ttcggtcgtc tcgtcgaaga gctgcgaggc cagcgccatc acctggtggg
     1081 catggtgcgc ctcgtagtgg tagcgcatcg cgaacgaaac gagattgcgc tcgcgcacgg
     1141 tatgcgggtc gtagcgatct cccttccccc cgcccgcaat gcgcgccagt cccttcacgt
     1201 gatcgagcgc ggtggcctcc atccgctggc gaatgtggtc gaggatcatc ccgtcccgga
     1261 tcgagcgatc gggaatgtgc acccggtcat tggccactcc ctcgaggacc gtcatcagga
     1321 tggcgccacc cggcacgatg atgtcggcgc gcggactctg ctcggccatg tagtcgcgcc
     1381 gttcgtcgag ggccagcgtg gccaggtagt gaagctcacg cgtgagctcc tcgcggctca
     1441 cggagaaggt ctcaccgcgg gagagcgtgc ggcccgcccg gcgtgcggcc agcatgccga
     1501 ggcagacgat cgtgcccgag gtgcccgcca cctcgtcgaa cgcctggtcg cgcaggcgtc
     1561 ggtgcgccgc gcccattcct tcgcgggcgg ccgccatgta ttccctgatc cccttcttcc
     1621 ccgggcgatc cttcacgcgt acctcgtcgg ccaggcgcac ggcgcccatc cggaggctgt
     1681 cgatcacctg gggctcctcg tcattgagca ccgccagctc cgtcgaaccg ccgccgatgt
     1741 ccaccaccag gtgcctgccc cggccgaacg gtggaagccc cgccacggcc accgccacca
     1801 gccgtgcttc ctcggccccc gcgatgcgat cgatcttgag cccggtccgc cggcgcactt
     1861 cccggaggaa ggccgtcccg ttcgaggcct cgcgcacggc gctggtggcc acgcccacca
     1921 ccacgtccac ttcaaagcgc cggatcacct cggcgaaacg ctcgagtgcc acgaatgcgc
     1981 ggcgttgcgt ggcggccgtc agttcgcgcc gggcgaatgc gcggctcccg aggcgcacca
     2041 tcgccttgtc cttgtggatg atctcgtagc cgtggtcggg aagcacacgg gcaatgagaa
     2101 gatggatgga gttcgagccg acatcgatgg ccgcgatctt cacgttgcac tccgtcgatt
     2161 ggagacgagc ggcctcggac tctggatcag tactggggca ggctcggatc gacctcgcgt
     2221 gcccagcggt cgatgccgcc cgtcaggttc ttcagccggc tgaaaccggc ccctcgaagt
     2281 atggccagcg ccttctggct tcgcaacccg gccttgcagt gaagcacgat gtcgtcggcg
     2341 ctggagagct cgtgcatccg ctccggcacc tggccgagcg gaatgagccg gctcccctgg
     2401 atccgattga tctcgtactc gtgcggctcg cgcacatcga tgatggtcag tttgtcgccc
     2461 cgatcgaggc gcgccttgag ctcggcgggc gcgatttcga actcggcctc tcccgtttcc
     2521 gcctcatgcg gtgtgatgcc gcagaactgc tggtagtcga tcagttcgtg gatcgtccgg
     2581 ttgggcccgc agatcgggca gtcaggattc ttcttcagct tgagctcccg gaacgacatc
     2641 cgcagcgcat cgaacaacag gagccggccg accagcgagg tgccggcgcc gaggatcagc
     2701 ttgacggttt cggtggcctg gatgacgccg atgatccccg ggaggatgcc gagcacgccc
     2761 ccctccgcgc atgaggggac cagccccggc ggcggcggct ccgggtagag gcagcgatag
     2821 cacggtccct tttccgccca gaacaccgag gcctgtccgt cgaagcggaa gatggagccg
     2881 tacacgttcg gcttctccag cagcacgcac gcgtcgttca ccaggtagcg cgtcgggaag
     2941 ttgtcggtgc cgtccaccac gatgtcgtac tcgcggaaga gctcaagcgc gttttcgctg
     3001 gtcagcgccg cttcgtacgt gtcgatccgg atgttcggat tcaggtcgcc gaggcgcgcc
     3061 ttcgccgatt cgagcttcgg ctttccgagc gaggaggtgc catgcacgat ctgccgttgg
     3121 agattcgtgg cgtcgacgac atcgaagtcg acgatgccga ggcgtcccac acccgccgcg
     3181 gtcaggtaga ggccgagggg actgccgagt ccgccggcgc cgatcatcag cacgctggcc
     3241 gccttcaact tcttctgccc ggccatggtc acctcgggca tgatgaggtg acggctgtag
     3301 cgatggatct cgtcgttgga gagggtcacc gcggccgcgc gggcgggaat gtcggccagt
     3361 cgtccggagg tcgggacttc cgtggagcgt ggatcggccg ccgccggtgt gggcgcggcc
     3421 ccacccgcga tcgagggaac gatcgtcagc tcgtctcccg gcttgatcgg cgtggcgccg
     3481 cccgacaggt gacggatgtc ctcgtcgttg acgtacacat tcacgaacga tcggaggcgt
     3541 ccctcgtcgt tgaagagatg cttcttgagg ccggggtgcg ttgcggtgag gcgggccagc
     3601 gcctcgtcca ccgtcacggc gtccaccgac acccggtcca cgccaccggc gtagggacga
     3661 aggggcgtcg gaatatggat cggggtcggc atcgtcagtc ctcccgcgcc ggcacgacag
     3721 gctcggcgtc gaaacgcgtc cggtcctccg ccagcgtcca ggagtcgagc tcccgggcgc
     3781 cgccgtcgac gaccgacaca atgatatagg agtaaaaggg ccaggcatgc tcgcgatcga
     3841 actcggacgg cacggccggc gcgttcgggt gcgaatgata gaaaccgagg atgtcgatcc
     3901 cgcgccggcg ggccgagcgg tcggccgcca ggaacgtctc gggcgtgatg aggaagcgat
     3961 tgtgacgcga ttcctcgcgt tcgttggcca gcggctccac cgtggtcacc cgcttggtcc
     4021 gtccgtccgg ctccactttt ccgaggagca gcccgcagca ttcgtgcgga aaggcctcct
     4081 cgccgtgccg gcggatcgct tccaggtcgg aggggcggat caggaggccg cccgcccggt
     4141 cggtccgagg ccgcggtgac gacgttccgc tcatgactcg tcctcttcgg tccagaatcg
     4201 ttcgccgaga taccgatcgc cgccgtcggg aaacaccgtc accacggtcc cccgcgacaa
     4261 ccggcgcgcc acgcgcaggg ccaccaccac cgccgcgcca ctcgacggcc ccacgcggag
     4321 tccttcctcg cgggccaggc gccgcgtcat ggcgtaggcc tcctcggtcc ccacctcctc
     4381 gtttcgatcg gccagagtcg gatcgaagat gccgggcacg atggcggaag gcatgtgctt
     4441 cacgccgtcc aggccatgaa acgggctgtc gggctggaag ctcgtgaggt gcacgtctgg
     4501 gccttccctc ttcaggcgcc ggcccactcc catgaaggtt ccggaggtgc caagcgccgc
     4561 cacgaagtgg gtcacccggc cggccgtctg ttcccagatc tcacggccgg tcgtatcgag
     4621 atgcgcacgc cagttggcgg gattcgtgta ctggtccgcc aggaagaagc ggccgggttc
     4681 cgcccgcgag agacgggccg cctcgcggat ggcgccgtcc gtcccctcca ggggatcagt
     4741 gaggaccagc tccgcgccgt aggcccgcag cgtggccagg cgttccggac tcaccctggc
     4801 aggcaggcag agtgtgacgc ggatccccaa caccgcgccc accattgcgt agccgattcc
     4861 cgtgttccca gaggtggcgt cgagaagcgt gccgtccggc ttgagccgac cgacacgcag
     4921 cgcctcgagc acgatgcgga gcgcggggcg atccttgacc gacccgccgg gattgaacca
     4981 ttcggccttg ccgaacacct ggacgtcggc cgggaggtcg cgggtcaccc ggttcaatcg
     5041 gaggaggggc gttgacccga cctgcgccag gagttcgtgg gcgtgtcgcg cgaggcgagg
     5101 ttcgtgccgg gcgaggtcgt cgatcagtcg catccggaag tctagcacgc gccccggcta
     5161 ttgttgctcc ccaaagtcaa ctttacatgt ccccgcccaa acccacctgt tcgtaaggag
     5221 ttaggtttcc ggccagggga acgggtggta ggctccctcc atgctcaccg aggaaccatt
     5281 cgagatcgaa ggagaagtcg gcgggtccat ccgaggcgac gtccgctggg gacccgatag
     5341 tggaccgacc atcgtcatcg cccacgggtt caaaggtttc aaggactggg ggcattttcc
     5401 gttcgtctcc cggcagttcg cgggggccgg cttccgggtc gtctcgttca acttttccgg
     5461 ctcgggaatc ggcgagaagc cgaccgagtt caccgagctc gaccgcttcc ggcacaacac
     5521 gttgagccgg gaggtccacg aactggatcg gctcatcacc tccgccgcgg caggccggct
     5581 ccccggcggc gccacgatcg gcagggttgg tcttctcggt cacagtcgcg gggccatcgc
     5641 caccacggtc gtggccgacc ggcggtccga tatcggcgcg ctggtcacgt gggcgggggt
     5701 cggcgtgctg cgcgagcgct atcccgacga cgtccgacgg gcctggcgcg aaaccggcga
     5761 gctgccggtg gtcaacgcgc gcaccggaca atcaatgccg atcggcctcg aggcgctgga
     5821 tgatctcgag cagcatctcg acgactattc gcccgatgtg atatccgccg ggctcgacgt
     5881 gccgcatctc ctcatccatg gcgatgccga cgccggcgtt ccgatcgagg agtcgcgcgc
     5941 ggtgtcccgg gcgtcccatg gaaagtcgcg gctcatcgag atacccggcg ccgatcacac
     6001 cttcggctcg gtccatccgt ttcagggtga gggtccggca ctcatggaag tcatcgagca
     6061 cacgctcgcg tttttcggcg aacacctgtc gaagcaagcg cggtgagggc gggaaataga
     6121 aaaggatact tcccgcattc accgtggtcc ggtcgcctaa gcaccaggcg ggaaacccca
     6181 ttttccggaa cacgagagcc gcaggaagta tccttaaaag cctgccgatc gcgatggagg
     6241 attcgctacc agctatgttg accggccata cacccggctc cgatggaaag ttcccggggc
     6301 gcgcccatcc gggcgaaatt gctgctaaat ccttgacttt ccgcgggctg ccgggcctct
     6361 ccggcggtgg ccgagcgacg gcaggtcccc gatgaaacga cattcaccca tcgattttcc
     6421 ctatggccgg gatcggatca cctgggtccc cgacccggca ctcgacgtca ccctggtcga
     6481 gccgcgcgaa ccaccccccg cccaggatcc gggccgcctg atccgcgagg cgctcgagaa
     6541 tccgctcggc tcaccgttgc tcgccgaact ctcacgcggc gcaaaatcgg ccacgatcgt
     6601 ggtgacggac gccacgcgtc cctgtcccga tcggctcctc gtgacgccga tcctcgaaac
     6661 gctcgccgcg gccggcattc ccgacaccgc catcaccttg ctggtggcca tcggaatgca
     6721 ccgtcccagc acgccggagg aaaagatcga gaagctcggc gatgccatcg tgtcgcgcta
     6781 tcgcgttgtc gactcggcgc cgctcgatcc ccgacttcaa gtcgacttcg ggaccagccg
     6841 ggacggcgtt ccaatcacga tcgatcgccg cgccgcggac gcggatctcc tcatcgccac
     6901 cggcatcgtc gagccgcacc agtacgcggg cttcagcggg ggatggaaga cggtggcgat
     6961 cggcaccgcg ggcgcggcca ccatcacggc gctgcacggc atgcattacc tcgagcatcc
     7021 gcgcgttcga ctgggcatga cggatgacaa tccgttcctc gacacggccc ggtgggtcgg
     7081 tgaaaaggcc ggcctcaggt tcgtggtgaa cgtggcgctc gatcccaatg gggtcatccg
     7141 gcgcgtgcgc gccggccatc ccgagacggt gcatcgcgac atgtcggcct gggtgcgcgg
     7201 agaagccatc gtgccggtcg atggccaatt ccccatcgtc atcggcgcgg ccggtcatcc
     7261 caaggattcg aacctctacc aggcaacccg cattcccacc tacctctgct atgcgcgccg
     7321 ccccgtcgtg gcgcccggcg gggtgatcct ggtgccggcg gcctgtccgg aaggggcggg
     7381 gcagggggcg ggggaacgac tcttcgagga gatgctcggc caggggggtt cccccgacga
     7441 gatcatcgag cgtgcgcggc gcgagggata ccccgccggc ggccagcgcg ccgtcatggt
     7501 ggcgtgggcc atgaagaacg cccggctcgc cttcgtgggg acgatcgacc cggagctgcc
     7561 gcgccgactc gggatgctca gtttccggac cctcgaggag ggcgtcgaat gggccgtccg
     7621 cgacaccggc tccgagcgaa tcctcgcggt gccgcacgcc ctgatgacgc ttccggaaaa
     7681 atgtgagtaa gcacgatcgc agagagtacc gcgattgact taaactgctg tcttgtatgg
     7741 agtttctgga ttttccacat ttcgggcggt ttgtccacaa ggcggcccgc ccggaccacg
     7801 gtcagcgctg gccgagcaac cccaatccac gctgcgtggt agccgggtcg gcggcggccg
     7861 aaccgccgga accgtgagcc gatgacgcct cctcgatcac gacatcgtcc cccatggagc
     7921 aattcccctg gaagaagacc ccttcctcga tcatgaagct cttggcgagc acgtcaccct
     7981 caagtcgagc gcctgtctgc agctccacgc ggtccttcgc caccagtttg ccgcgcacct
     8041 tccccgcgac gaggatgtca cgcgccgtga ttccggcccg cacgttgccc gtcttgccga
     8101 caatcgcgga ctccgtggcc gtcagcgttc cttcgaactc gccatgcacg aggatgcttc
     8161 cttccacgcg cagcgtgccg gtcagcgaag tcccttcgcc gatcacggta ttgattctcg
     8221 tatccgccgg ggcaccgccg tccttcttgg ccgaaccgaa catcgccatg agtcctcctc
     8281 ccgcctcgcg cgtcagtcgc gcggtgtccg ttccccgatc aaaccggcga tttcgagcac
     8341 ccgctcgaga tcgctctcac tgaagtagct gatttccacg cgcccgcggg ccgtcccgcc
     8401 gaggatgcgg acctgcgttc cgtagacccg ccggagccga tcttcccagt ccgccagcgc
     8461 cggcgcgaca cgcccccgtc gcccggtgcc acgcggacgg gtctgccgat gccccgcggt
     8521 catctcttcc acctggcgga cggaaaagcc gcggcgcacg atatcgctgg ccatcgcttc
     8581 ctgctgggtg gccgactcga tggccaggag gcaccgcgca tgccccgtcg acagcttgcc
     8641 cgccgaaagc atctcctgca ccgcctcggg cagattcaat agtcgcaggg agttggccac
     8701 cgtggtgcga tcctttccca cgcgggtcgc aatctgttcc tgggtcaggt cgaactccga
     8761 cgccatccgc tggtaggcgt gcgcctcctc gatcgggtcc aggtcttcgc gctggatgtt
     8821 ctcgatcagc gcgaactcca gcatctcctt gcgcgaggcc atgcggacca cggccggcac
     8881 cgagtcgcgt cccgccagct cggcggcctt cagtcgacgc tcgccggcga tcaattgata
     8941 tccccgtccg tccggcgcat gacgaacgat taccggctgc aggacgccgc tcatccgtat
     9001 cgactcggac agctctcgaa ggcgttcggc atcgaagtga tggcgcggct ggtaagggtt
     9061 gccgctgatc gacgcgatcg ggaggtcgat gggggttccc gcgggcgtgg ccaccgcgac
     9121 ggcggccggt cgatcggatg ggccggccga cgacgtcggc gtgggcgcct ccggaatgag
     9181 ggcgccgagc cctctaccca gtgctttgcg tgtcatggtt cagcacttcc tttgcgagat
     9241 tgaagtaact ctcggcaccc gcgctcttcg gatcgtaggt cacgaccggc tggccgaagc
     9301 tcggggcctc gctgatgcga acgtttctcg ggatgacgct ctggtaaacc ttccctggaa
     9361 aaaacttgcg ggcctcctcg gaaacctggt gcgacaggtt caggcgtgcg tcaaacatcg
     9421 tgaggaggat cccttcgatt tccagcgtgg ggttgagccc ccgctgaatg agccggatgg
     9481 cgttgaggag ctggctcaat ccctccagcg cgtagtactc ggtctgaagc ggaatgagga
     9541 ccgagtcggc cgcggtcatg gcattgatcg tgaggaggcc aagggaggga gggcagtcga
     9601 taaggatgta atcgtacgca tcgcggaccg cttcgagccg ccgcctcagg acccgctcgc
     9661 gttcgggcac gggaaccagt tcgatctcgg cgccgatgag cctctgcgcc gcggggacga
     9721 cgtgaagggt ggcgatgtcg gccggctggc ggaccgattc gagggggagc tcgttgacga
     9781 gcacctcgta gatcgtctcc tccagctcgt tcccgttcga gccgagggcg ctggtggcat
     9841 tcccctgggg gtcgatgtcg accagcagga ctcgccgacc cgccagcgca agggcggctg
     9901 ccagattcac ggtggtggtc gtcttgccga ccccgccctt ctgattggct accgcgacaa
     9961 cccgtgccat gtgtggaccg cccccacagc attaagtgtt gaagagttca gtggttgttc
    10021 cagtcggggt gtcgggtcgc cggtgcgcca gcatcttcgg ccattcgctg cgcgaagtca
    10081 acggtttcaa acgtgttgat ggtggagcgg ccggattgtt tcacgtgaaa cggcaggggg
    10141 gcgggggttg ttttcacgtg gggcagagtc caccactgtt tcacgtgaaa cagtgcggct
    10201 cgattccagg cggctgtgcc tacccgagcg tcaggaagag cagctcccga ggttgaccag
    10261 gcatcgctac gcgtcgaacc gcagccgggg ccgggtcgag ctccgccagc gattcccctc
    10321 cttcgccggg ggcgcgatac atggccagct cgccgccgga acgcagcagg ccggaggccc
    10381 aactccacac ttgctccagg cggtcgacgg cccgggcggt agccagcccg gcaccaagtt
    10441 ctcct
//
LOCUS       DASVOD010000360         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_754457, whole genome shotgun sequence.
ACCESSION   DASVOD010000360 DASVOD010000000
VERSION     DASVOD010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754457"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="VF720_08345"
     CDS             complement(<1..1943)
                     /locus_tag="VF720_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX7879404.1"
                     /translation="MHVNILGGGPAGLYLSLLLKKADPAHRVAVFERNRPGDTFGWGV
                     VFSDQTLGNLKGADEPTYQAIADSFAHWDDIDIHFKGRTITSSGHGFCGISRHKLLAI
                     LERRAADLGVELNFSTEVPDPHALRDADLLVGADGVKSVVRAQHADVFQPSLDVRSAR
                     FIWLGTTRLFDAFTFIVVETPHGVFQVHAYRFDREHSAFIVECDEASFVRAGLDKHDA
                     AAAVRFSEEMFAPWLDGHRLIHNAPHQAAEPWLRFTVVKNATWHHGNTVLIGDAAHTA
                     HFSIGSGTKLAMEDSIALAHHLHAAPSLPEALASYEGERMSEALRLQNAARNSMEWFE
                     NVPRYVGLEPEQFAYSLLTRSQRVTHENLRMRDAQYVSAVERWFAAGTEPVPPMFTPY
                     RLRELTLANRIVVAPMDMYSAVEGVPNDFHLVHLGSLALGGAGLIHTEMTCPSLEGRI
                     TPGCTGIWNEEQAAAWRRIVDFVHANGAAKICLQLGHSGRKGSTNLMWVDMDGPLPDG
                     NWPVVGPSALAYKPGMQVPAEITRAQMDTIRDEFVAAVRYGLSAGFDMIELHAAHGYL
                     LSSFITPVSNRRTDEYGGSLENRLRWPLEVFRAMRGAWPGARPMSVRISASDWVAGGL
                     SGEESVDVARAFHAAGADIIHVST"
     gene            2178..>2716
                     /locus_tag="VF720_08350"
     CDS             2178..>2716
                     /locus_tag="VF720_08350"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7879405.1"
                     /translation="MTAATEDRELVDRAIGGSLAAWKEIYETTRPKLFGFLVYQLGNR
                     EESLEILQETYLAAMASIKGYRGDAPLESWLLGIAIRKTRHWMRRAARWRRRFEPMPM
                     PDDSPGDDDPSRRLVGRQLREAIATLTEAQRTAFLLHVWFEYPFADIAAVMRVREATA
                     RVHAHRAKESLKRRLGPEAL"
BASE COUNT          472 a          936 c          878 g          430 t
ORIGIN      
        1 gtcgacacgt ggatgatgtc ggcgccggcc gcgtggaagg cgcgggccac gtctaccgac
       61 tcttcgccgg acagaccgcc ggccacccag tcgctggcgg agatgcgcac cgacatcggc
      121 ctggctccgg gccacgcgcc ccgcatcgcc cggaacacct cgagcggcca gcgcagacgg
      181 ttttccagcg agccgccgta ttcatcggtt cgccgattgc ttactggcgt gatgaagctc
      241 gacaggaggt acccgtgcgc cgcgtgcagc tcgatcatgt cgaatccggc cgacagtccg
      301 taccgaaccg cggccacgaa ctcgtcgcgg atggtgtcca tctgcgcccg cgtgatctcg
      361 gccggcacct gcatcccggg cttgtacgca agggcgctcg gcccgaccac cggccagttc
      421 ccgtcgggga gcgggccgtc catgtccacc cacatgagat tggtggagcc cttgcgaccc
      481 gagtgcccca gctgcaggca gatcttcgcc gcgccgttgg catgcacgaa gtcgacgatc
      541 ctccgccacg ccgccgcctg ctcctcgttc cagatgccgg tgcatcccgg cgtgatcctt
      601 ccctcgaggc tcgggcaggt catctcggtg tggatgagtc cggcgccgcc cagcgccagc
      661 gaaccgaggt gcacgagatg gaagtcgttc ggcacgccct ccaccgccga gtacatgtcc
      721 atcggcgcca cgacgatgcg gttggccagc gtgagttcgc gaagccggta cggggtgaac
      781 atcggcggca ccggctccgt gccggcggcg aaccagcgct ccaccgcact cacatattgc
      841 gcatcgcgca tccgaagatt ttcgtgcgtg acgcgctggc tccgcgtgag gaggctgtac
      901 gcgaactgct ccggctcgag gcccacgtag cgtggcacgt tctcgaacca ctccatcgaa
      961 ttgcgggccg cgttctgcag gcgcagggcc tcgctcatcc gctccccttc gtaggatgcg
     1021 agcgcttccg gtaatgatgg cgccgcgtgc aggtgatgcg ccagcgcgat cgaatcctcc
     1081 atggccagct tggtgccgga cccgatcgag aagtgcgccg tgtgcgccgc atccccgatg
     1141 agcaccgtgt tgccatggtg ccaggtggcg ttcttcacca cggtgaagcg cagccacggc
     1201 tcggccgcct ggtgcggcgc gttgtggatg aggcggtggc catccagcca gggagcaaac
     1261 atctcctcgc tgaagcgaac agccgccgcc gcgtcgtgct tgtcgagccc ggcccggacg
     1321 aacgaagcct cgtcgcactc cacgatgaat gccgagtgct cgcgatcgaa gcgataggca
     1381 tgcacctgga agacgccatg cggcgtttcc accacgatga acgtgaacgc gtcgaacagc
     1441 cgggtcgtcc ccagccagat gaaacgcgcc gaccgcacat cgagcgacgg ctggaagacg
     1501 tcggcgtgct gtgcccgcac cacactcttc acgccgtcgg ccccgaccag gaggtcggcg
     1561 tcccggagcg cgtgggggtc gggcacttcg gtgctgaaat tcagctcgac gcccaggtcg
     1621 gcggcccgac gctcgaggat ggccagcagc ttgtgccgcg aaatcccgca gaagccatgg
     1681 cccgacgacg tgatcgtccg tcccttgaag tggatgtcga tgtcgtccca gtgcgcaaag
     1741 ctgtcggcaa tcgcctggta ggtcggctcg tccgcccctt tcaggttccc cagggtctgg
     1801 tcggagaaca ccacccccca cccgaaggta tccccgggcc ggttccgctc gaacaccgcc
     1861 acccgatgcg ccggatcggc cttcttgaga agcagggaga gatagaggcc ggcgggtcca
     1921 ccgccgagga tgttcacgtg catggggttg gagggtaccc gaagtgaggg caggtcggaa
     1981 tcgaggccgg gttgtcccgt ccgatatcgc tgaaaaatac gccgatgttc gttttactct
     2041 cctcgaggag agttcggcac caatcagcct gcaccgtgta aacgaaatca ctccgtccgg
     2101 gcactgaaca cacgaatccc tcaggaaggc tcgagcgacg agccggtatg gacccgggcc
     2161 ccgggagtgc acgaacgatg acggctgcga cagaggacag ggagctggtg gaccgcgcga
     2221 tcggcgggag cctggcggcc tggaaggaaa tttacgagac gacccggccg aagctcttcg
     2281 gcttcctggt gtatcagctg ggaaaccggg aggaatcgct cgagatcctc caggagacgt
     2341 acctggcggc aatggcctcg atcaagggct accgcggaga cgcaccgctc gaaagctggc
     2401 tcctcggcat cgccatccgg aagacacgtc actggatgcg acgagccgct cgatggcgcc
     2461 ggcggttcga gccgatgccg atgccggacg actcgcccgg cgatgacgat ccctcgcggc
     2521 gcctcgtcgg ccgccagctc cgcgaggcca tcgccaccct caccgaagcc caacgcaccg
     2581 ccttcctcct ccacgtctgg ttcgaatacc ccttcgccga catcgcggcc gtcatgcgcg
     2641 tgcgggaagc caccgcccgc gtgcacgccc accgcgccaa ggaatcactg aagcgacgcc
     2701 tcggcccgga agccct
//
LOCUS       DASVOD010000361         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_650008, whole genome shotgun sequence.
ACCESSION   DASVOD010000361 DASVOD010000000
VERSION     DASVOD010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650008"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..2252)
                     /gene="recG"
                     /locus_tag="VF720_08355"
     CDS             complement(51..2252)
                     /gene="recG"
                     /locus_tag="VF720_08355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX7879406.1"
                     /translation="MPTATKRTRPPAAADSRPPAQRGARSSAQAGARSSAAAPQTGTD
                     SGPDTPVQYLKGAGPRRAETLARLEIRTVRDLLYHFPRAWADRTRLDTIASAEVGQDA
                     TLLATVRRVDVPRRRGRADTVVTLEDGTGVLHAIWFGQPYMKRNFEPGQRLMLSGMVG
                     FHDRKRLSNPEWEIWSDDADSAHVGRIVPIYPSTAGMSQRVLRGLVRQALAMCLERIE
                     ETLPRSICDAESLTARRDALRTMHFPERMDDIEAARHRFIFEEALEIQMLLRWVRLER
                     ETRRPGIAFPMESPLARKLEGSLPFTLTDDQQKALAEIIDDMRRPTPMGRLLQGDVGS
                     GKTVVALLAAAHAVDAGFQAAIMAPTEVLAEQHRATFERLAGPLGIPIVRLTGQVKSA
                     ERARVLEAIRTGAAAIAIGTHALIQEGVEFHNLGLVVVDEQHRFGVLQRADLKTKGRT
                     PDLLAMTATPIPRTLYLTKAADLKLSQIRTRPAGRGKVVSRVTTEANRDKVYEVLARE
                     IGRGRQAYVVYPIIEESEKLDLKAATAMYETLKSHQALSGLRIGLLHGRMKSHEKGSM
                     LDSFRGGTIDLLVTTTVIEVGIDVANATVMVIEHPERFGLSQLHQLRGRIGRGSETSY
                     CILLAEGAGGPAHERLRLFETTQDGFALAEADLRFRGAGSILGVRQSGPAGMSLGIVD
                     PVRDEAVLERAHMLADRLAIDDPHLLDPAWREWRARLKKVLDDSRRFLDAG"
     gene            2385..2597
                     /gene="rpmB"
                     /locus_tag="VF720_08360"
     CDS             2385..2597
                     /gene="rpmB"
                     /locus_tag="VF720_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX7879407.1"
                     /translation="MARNCEVCGKGSQWGHKVSHAHNVSNRRWYPNLQSVRALKEGKP
                     TRMRVCTRCLRSDKVAKNVRKPTAAA"
     gene            complement(2698..>3479)
                     /locus_tag="VF720_08365"
     CDS             complement(2698..>3479)
                     /locus_tag="VF720_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879408.1"
                     /translation="GDPNGARAAFGAALTLNPNYQRARFYQGLAAFLAGDEPGGLNDL
                     RASVDGQVRFLPEAIDLGSVLLKRGEYDSAVQHFERAVEIHPDYADLHFGHGAAHLGT
                     GNAPAAAESFRRAVAINGKFLQAHLHLGKALAAGGDLTGARVSFERALELEPKLADGW
                     CLMAQTEVRLDSLRSAEAAYRRALTINANYVAALLGLGSLLARRGEAAEAEQVLGRVL
                     AIDPLNTAARALLSPLPPEALETESGGGAPGGVAPGGVAPD"
BASE COUNT          522 a         1169 c         1203 g          585 t
ORIGIN      
        1 cgggtcttgc actcgggaca gcgcagatac atgggtgtcg ttccgcccgg tcagccggcg
       61 tcgagaaaac gccggctgtc gtcaaggacc ttcttgagtc gcgcgcgcca ttcgcgccac
      121 gcggggtcga gcaaatgggg atcgtcgatg gccagtcgat ccgccagcat gtgggcccgt
      181 tccaggacgg cctcatcgcg cacgggatcg acgattccga ggctcatgcc ggcgggaccg
      241 ctctgacgaa cgccgagtat gctaccggcc ccgcggaacc gcaagtcggc ttcggccagg
      301 gcaaagccgt cctgcgtcgt ctcgaacagg cgcaaccgct cgtgcgcggg tccccccgct
      361 ccctccgcga ggaggatgca gtacgacgtt tccgagccgc ggccgatccg tccccggagc
      421 tggtggagct gcgagaggcc gaaccgttcg gggtgctcga tgaccatgac ggtggcattg
      481 gcgacgtcga tgccgacttc gatcacggtg gtcgtgacca ggagatcgat tgtgccgccg
      541 cggaaggagt cgagcatgct ccccttctcg tggctcttca tccgcccgtg gagcaggccg
      601 atcctgagcc ccgacagggc ctgatgtgat ttcagggtct cgtacatggc ggtggcggcc
      661 ttcaggtcga gcttttccga ctcctcgatg atcgggtaca ccacataggc ctgtcggccc
      721 cggccgatct cgcgcgccag cacctcgtac accttgtcgc ggttggcctc ggtggtgacg
      781 cggctcacca cctttccgcg cccggcgggc cgcgtgcgga tctgactcaa tttgaggtcg
      841 gcggccttgg tgagatacag ggtgcgcggg atcggggtgg ccgtcatggc caacaggtcg
      901 ggcgtgcgcc ccttggtctt caggtcggcc cgctgcagga caccgaagcg gtgctgctcg
      961 tcgacgacca cgagccccag gttgtggaac tccacccctt cctggatcag ggcatgcgtg
     1021 ccgatggcga tggccgccgc gcccgtccgg atggcctcga gcacgcgggc ccgctcggcc
     1081 gacttcacct gtccggtgag ccgcacgatc ggaattccga gcggcccggc cagccgttcg
     1141 aacgtggccc ggtgttgctc ggccagcact tcggtggggg ccatgatcgc ggcctggaag
     1201 ccggcgtcga ccgcgtgggc cgcggcaagg agcgccacca ccgtcttgcc gctgccgacg
     1261 tccccctgga gcaatcgccc catcggcgtc gggcgccgca tgtcgtcgat gatttcggcg
     1321 agcgccttct gctggtcgtc ggtgagcgtg aacggaagtg agccttcgag cttccgggcc
     1381 aggggcgact ccatgggaaa cgcaatcccc ggccggcggg tttcccgctc gaggcgcacc
     1441 catcgaagga gcatctggat ttcgagcgct tcctcgaaga tgaagcgatg gcgtgccgcc
     1501 tcgatgtcat ccatccgctc cgggaaatgc atcgtgcgga gggcgtcgcg gcgggcggtg
     1561 agcgattcgg catcacatat cgaccgtgga agtgtctcct cgatccgctc gaggcacatc
     1621 gcgagcgcct ggcgcacgag gccccgcaac acgcgctggc tcatcccggc ggtggaggga
     1681 tagatgggaa cgatgcggcc gacatgcgcg gagtcggcgt cgtcgctcca gatctcccac
     1741 tcgggattgg agagacgctt ccgatcgtgg aaaccgacca taccggaaag catgagccgc
     1801 tggccgggct cgaagttgcg cttcatgtac ggctggccga accagatcgc gtggagcacc
     1861 ccggtgccgt cctccaacgt cacgacggtg tcggcccggc cgcgccggcg cggcacatcc
     1921 acgcgccgga cggtggccag cagcgtggca tcctggccga cctcagcgct ggcgatcgtg
     1981 tcgagccggg tccggtcggc ccaggcccgg gggaagtgat acaagaggtc ccggacggtg
     2041 cggatctcga gtcgggccag tgtctcggcg cggcggggtc cggcaccctt caggtactgg
     2101 accggggtgt ccggacctga atcggtacca gtctgcggtg cggcggccga ggatcgggcg
     2161 cccgcctggg ccgaggatcg ggcgcctcgc tgggccgggg gccggctatc tgctgcggct
     2221 gggggccggg ttctcttcgt ggccgtgggc attgttcgct tgatcgttcc cggggcttgt
     2281 gttagctttt tcgggctttc tgccggaaga agtccggcaa cccgaagtca ttggaatacg
     2341 ccgatttccg ctccagcgga cggcggtcgt agggagtgta ctcaatggcc aggaattgcg
     2401 aagtctgcgg caagggctcc cagtggggtc acaaggtcag ccatgcccac aacgtctcga
     2461 atcgccgctg gtatccgaat ctgcagagcg tccgcgccct gaaggaaggg aagcccaccc
     2521 gcatgcgggt ctgcacgcgc tgcctccgtt ccgacaaggt ggccaagaac gtccggaagc
     2581 cgacggccgc cgcgtagcct gattcgacac caataagccg agcccaacgg ccgccactca
     2641 cactgagtcg gcggccgtgt tcgttggtcc ggtcggttcc ggtcagatcg actccggtca
     2701 gtccggcgcc actccgcccg gagccactcc gcccggagcc ccaccgcccg attccgtctc
     2761 cagcgcttcc ggcggcagcg gcgacaggag cgcccgggcc gcggtgttca gcggatcgat
     2821 ggccagcacg cggccgagca cctgttccgc ctcggcggcc tccccccgcc gcgcgagcag
     2881 gcttcccagc ccgaggagcg ccgccacgta attcgcgttg atggtcagcg cgcgccgata
     2941 ggcggcctcg gcactccgga gcgaatcgag tcgaacttcg gtctgggcca tcaggcacca
     3001 gccgtcggcg agtttcggct cgagttcgag cgcccgttcg aatgacacgc gcgcgccggt
     3061 caggtctccc ccggccgcga gggctttccc caggtgaaga tgcgcctgca ggaacttgcc
     3121 gttgatggcc accgcccgcc ggaacgactc ggcggctgcc ggggcgtttc cggtcccgag
     3181 atgggcggcg ccgtgtccga agtgcaggtc ggcgtagtcg gggtggatct caaccgcgcg
     3241 ttcgaagtgc tgcacggcgc tgtcgtattc gccccgcttc agcagcaccg agcccaggtc
     3301 gatcgcttcg gggagaaagc gcacctggcc atccacggag gcccggagat cgttcaaccc
     3361 tcccggctcg tctcccgcga ggaacgcggc aagtccctgg tagaagcgcg cccgctggta
     3421 attcggattc agggtcagcg cggcgccgaa tgctgctcgc gcgccgttcg gatcgccat
//
LOCUS       DASVOD010000362         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_233497, whole genome shotgun sequence.
ACCESSION   DASVOD010000362 DASVOD010000000
VERSION     DASVOD010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233497"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VF720_08370"
     CDS             <1..482
                     /locus_tag="VF720_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histamine oxidase"
                     /protein_id="HEX7879409.1"
                     /translation="VRESDMAAGRAWIISNPDSLNRLGDPVGYKLHGQGHPMLLADPQ
                     SSIAQRAAFATKDLWVTRYDENERYPSGDFVNQHSGGAGLPAYIARDRDIDGQDIVVW
                     HTFGLTHFPRPEDWPIMPVDTVGFKLRPEGFFDRSPVLDVPPSKPASGGHCHMEAQK"
     gene            479..>1924
                     /locus_tag="VF720_08375"
     CDS             479..>1924
                     /locus_tag="VF720_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7879410.1"
                     /translation="MSLTTGKETTEVGQPHHHRRRLGVPAVTFMIIAASAPLTVLAGG
                     VTTTFAVTGVTGVPLSFLLLGGTLAVFAVGYAAMSRYVVNAGAFYSYVAQGISRPVGV
                     GVSMVALMAYNLMQVGIYGLFGFTVSSLINERFGITIPWWIPVLACIAVVAFLGVNRV
                     DLSAKVLGVLVGLEFLVVIVYDVVCFAVAPEGVSGAPLSTQSLFVPGVGAVLSFGIAA
                     FMGFESAAIYGEESKDPKHTVARATYTAVAIIALFYAVSSWAMAVGTGPSAVVDASAK
                     QGPDLMFGFLSDHAGVLLADLARLLFVTSLFAALVSFHNAAARYFFSLGREQVLPPKL
                     GAVRRHSGAPYAGSIAQTVIAVVVTVAFAIAGTGSSLGDLYPVLTMFTWLTNDGAMGL
                     VLLMTVVSLAVIGFFRKNRHDAGIWTRLIAPALGFVLLAAIFVLIVVNFNVLLGQNET
                     NALTLVLPATIIVPGLVGIAWAFRLRRSNPAV"
BASE COUNT          300 a          679 c          566 g          379 t
ORIGIN      
        1 ccgtgcgcga gtctgacatg gcggcgggac gggcctggat catctccaat cccgactcac
       61 tcaaccgcct gggcgacccc gtgggataca agctccacgg acaaggccat cccatgctcc
      121 tcgccgatcc ccaatcgtcc atcgcccaac gcgcagcctt cgccacgaag gacctatggg
      181 tgacccgcta tgacgagaac gagcgctacc ccagcggcga cttcgtcaac cagcactccg
      241 gaggagccgg actgccggcc tacatcgccc gcgataggga catcgacgga caagacatcg
      301 tcgtatggca caccttcggg ctgacccact tcccccgccc cgaggactgg ccgatcatgc
      361 ccgtcgacac ggtgggattc aaactccgcc ccgaaggttt cttcgaccgg agccccgtcc
      421 tcgacgttcc gccgtcgaaa cccgcctcag gcggccactg ccacatggag gcccagaaat
      481 gagtttaaca accgggaaag agaccactga ggtgggccaa cctcatcatc atcgacgtcg
      541 tctcggcgtc cctgcggtga ccttcatgat catcgccgcc tcggctccgc tcactgtcct
      601 cgccggcggc gtgacgacga cgtttgccgt caccggagtc actggcgtgc cgctgtcctt
      661 cctgctgctc ggcggaaccc tcgccgtgtt cgccgtcggc tacgcggcca tgagccggta
      721 cgtcgtcaac gccggcgcct tctattccta cgtggcgcag ggcatctccc ggccggtcgg
      781 cgtcggggtt tccatggtgg ccctgatggc ctacaacttg atgcaagtcg gcatctacgg
      841 cctcttcggc ttcaccgtct caagcctgat caacgaacgc ttcggcatca ccatcccgtg
      901 gtggattccg gtgcttgcct gcatcgccgt cgttgcattc ctcggcgtga accgcgtgga
      961 cctgtcagcg aaggtcctcg gggtgctcgt cggcctggag tttctcgtgg tgattgtcta
     1021 cgacgtcgtc tgctttgccg tggcccccga gggagtcagc ggagccccgc tcagcacgca
     1081 gagccttttt gtgccgggcg tcggggcggt cctctccttc ggcatcgcgg cgttcatggg
     1141 attcgagtcg gccgcgatct acggcgagga aagcaaggac cccaagcaca ccgtagcccg
     1201 ggctacatat accgcggtgg ccatcatcgc cttgttctac gccgtttcct cctgggccat
     1261 ggcggtcgga accggaccgt ccgcagtggt ggacgcttcc gcgaaacaag gaccggacct
     1321 gatgttcggt ttcctcagcg accacgccgg cgtacttctc gccgatctgg cccgcctgct
     1381 cttcgtcacc agcctcttcg cagctctcgt cagcttccac aatgccgcag cccgctattt
     1441 cttctccctg ggccgggaac aagtcctgcc gcccaagctg ggcgcagtac ggcggcacag
     1501 cggcgccccc tacgccgggt cgatcgccca gacggtgatc gccgtcgtgg tcacggtggc
     1561 tttcgccatc gcggggaccg gatcatcgct gggcgacctc taccctgtcc tgaccatgtt
     1621 cacgtggctg acgaacgacg gcgccatggg acttgtcctc ttgatgaccg ttgtctcgct
     1681 ggcggtcatt gggttcttcc gcaagaacag gcatgacgct ggaatctgga cccgcctgat
     1741 cgcccctgcc ctggggttcg tgctccttgc ggcgatcttc gtgctgatcg tggtcaactt
     1801 caacgtactg ctcggccaaa acgagaccaa tgcgctgacg ctcgtcctgc ctgccacgat
     1861 catcgtgccc gggctggtgg ggatcgcctg ggcgttccgc ctccgccgga gcaacccggc
     1921 cgtg
//
LOCUS       DASVOD010000363         4288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_389184, whole genome shotgun sequence.
ACCESSION   DASVOD010000363 DASVOD010000000
VERSION     DASVOD010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389184"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3507)
                     /locus_tag="VF720_08380"
     CDS             complement(<1..3507)
                     /locus_tag="VF720_08380"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7879411.1"
                     /translation="MSPHVVRPFLAALLVAVLAGGTRAALPEPDEFDGPDASGSEVVP
                     AWGGASCPCHQAEAWMASLGTFSVTGALELTGAPGQTHLRSLGEAPGDSAAADSTKSA
                     AKKDDGDSKEWFVNMAHGPSDTVRFTVSEGTWMSSDVSPDGRTLIFDLMGDLYTLPLS
                     GGKATRITSGPAWDSQPRWSPDGKRVSFTSDRGGCDNIWVMNADATHLRPVTQEKKVM
                     NTASWSPDGLWLVAKKRLTDYSSIGTAELWMVNTRGGSGLPVTVKGELPEAGEPVVSP
                     DGRYVYFSARPSRYAYNRNVFAGIYQIRRWDRTTGDQVTLTDGYGGSGRPQLSPDGKT
                     LAFVRRDRLKSVLYLYDIDRRSERPVWDGLDQDMQENFAWTGIYPGFSFMPDGKSVVI
                     NAQGGFFRINTTTGAATKIPFTADVEQVVSKALRFPRDIGSDTFRVRMISWPTQSPDG
                     KSLVFAAIGSLWSMPLPNGTPKKLATSGGLAYAPSFSRDGKWLTYVSWSDADGGHIWK
                     MPASGGGSTRLTSESSQYSNPAFSPDGSKIVAITGDNSPYRGHDLGGESYQQVVWVPA
                     SGSNNATHWVVDIPTRGSARRMPRPFFNADGTRIYYLETQGDGPGQEHSSLWSIRNDG
                     LDKIEHLKFEYAEEVVPSPDGNWAAYTEQFNAYLTALPGSGKKALDISGEGGPVPVKQ
                     FTGDQGAEWVNWANGGKTITWSYGPNFYRVSLDSVLAGWDRDMLKAGDKAMPKPKDKT
                     KDDDKGAAKGGDGKGVVDADSTAAKPDSAMAKAAEKKEPAEPTLAVDTLVVNLRLPRA
                     RPAQFLTMALTNARLITMKGDEVIENGTIVVQGNTITAIGPSSQVTVPSGARVIDCAG
                     KTIMPGMVDTHAHLHYNAMDIIPENQWPYWCNMAYGVTTTHDPSASTYAVFTQSEMVE
                     AGVMKGPRTFSTGYILYGAESPGKAVVNSREDAKNHIKRLKELGAFSVKSYMQPRREQ
                     RQWIIDAARAESVLVVPEGGGKFEENLSMVLDGHTSIEHSLPVTPLYSDVTQLFGHAG
                     TVETPTLLVAYGGLSGEHWFYQHYQPWEDRKLLTFTPRGIIDSRSIRRPVMTMDGDWH
                     HIKVSEGCLKIVDAGGKVTLGGHGQLQGLGPHWELWGLTQGGMTNHQALRCATYNGAW
                     SLGLDKWIGSLETGKLADLIVMD"
     gene            complement(3735..3878)
                     /locus_tag="VF720_08385"
     CDS             complement(3735..3878)
                     /locus_tag="VF720_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879412.1"
                     /translation="MSTIDTMELAVLVLGMVILMWDNRFQDGGARVSNSRPSRRGSAH
                     RPR"
BASE COUNT          655 a         1353 c         1523 g          757 t
ORIGIN      
        1 gtccatcacg atgaggtcgg cgagcttgcc ggtctcgagg cttccgatcc acttgtcgag
       61 gccgaggctc caggcaccgt tgtacgtggc gcagcgcagt gcctggtggt tggtcatgcc
      121 gccctgggtc agaccccaca gttcccagtg cggccccaga ccctggagct ggccgtggcc
      181 gccgagcgtc accttgccgc cggcgtccac aatcttcagg catccttccg acaccttgat
      241 gtggtgccag tcgccgtcca tggtcatgac ggggcgacgg atggagcggc tgtcgatgat
      301 gccgcgcggg gtaaacgtca acagcttcct gtcctcccac ggctggtagt gctggtagaa
      361 ccagtgctcg ccggagaggc cgccgtaggc caccagcagc gtgggtgttt cgacggtgcc
      421 ggcgtggccg aacagctggg tcacatcgct gtagaggggc gtgacgggga ggctgtgctc
      481 gatcgacgtg tggccgtcga gcaccatcga gaggttttcc tcgaacttgc cgccgccttc
      541 gggaaccacc aggacggact cggcgcgggc cgcgtcgatg atccactggc gctgttcgcg
      601 gcggggctgc atgtacgact tcacggagaa ggcgccgagc tccttgagcc gcttgatgtg
      661 gttcttcgcg tcctcgcggc tgttgaccac cgccttgccg gggctctcgg cgccgtacag
      721 gatgtatccg gtcgagaagg tgcgcggccc cttcatgacg ccggcttcca ccatctcact
      781 ctgcgtgaag acggcgtagg tgctggccga ggggtcgtgc gtggtcgtga cgccgtaggc
      841 catgttgcac cagtagggcc actggttctc ggggatgatg tccatggcgt tgtagtgaag
      901 atgcgcgtgg gtgtcgacca tgccgggcat gatcgtcttg ccggcgcagt cgatgacgcg
      961 ggcgccggac ggcacggtga cctgcgatga cgggccgatg gcggtgatcg tgttcccctg
     1021 caccacgatc gttccgttct caatgacctc gtcacccttc atcgtgatga ggcgggcatt
     1081 cgtgagtgcc atggtgagga actgggccgg cctggcccgc ggcaggcgaa ggttgaccac
     1141 cagcgtgtcg acggcgagcg tcggttcggc cggctccttc ttctcggccg ccttggccat
     1201 ggcggagtcg ggcttggcgg cggtggaatc ggcatccacg acgcccttgc cgtcaccgcc
     1261 tttcgcggcg cccttgtcgt cgtcctttgt cttgtccttc ggcttgggca tcgccttgtc
     1321 gccggccttc agcatgtcgc ggtcccagcc ggccagcacc gaatcgagcg agacgcggta
     1381 gaagttgggg ccgtacgacc aggtgatcgt cttgccgccg ttggcccagt tcacccactc
     1441 ggcgccctgg tcgccggtga actgcttcac cggcaccggg cccccttcgc cggagatgtc
     1501 gagggccttc ttgccgctgc ccggcagcgc ggtgaggtag gcgttgaact gctcggtgta
     1561 ggccgcccag ttgccgtccg gactcggcac cacctcttcg gcgtactcga acttcaggtg
     1621 ctcgatcttg tcgaggccgt cgttgcggat cgaccagagg gacgagtgct cctggccagg
     1681 gccgtccccc tgcgtctcga ggtaatagat gcgcgtgccg tcggcgttga agaaggggcg
     1741 gggcatgcgg cgggccgaac cgcgcgtcgg gatgtcgacc acccagtgcg tggcgttgtt
     1801 cgagccggag gccggcaccc aaaccacctg ctggtagctt tcgccgccga ggtcatggcc
     1861 gcggtagggg ctgttgtcgc cggtgatggc cacgatcttc gagccgtccg ggctgaaggc
     1921 ggggttcgag tattgcgacg actccgaggt gaggcgcgtc gagccgccac ccgaggccgg
     1981 catcttccag atgtggccgc cgtcggcatc actccacgaa acatacgtga gccacttgcc
     2041 atcccttgag aacgacgggg cgtaggcaag gccacccgag gtggccagct tcttcggcgt
     2101 gccgttcggg aggggcatcg accagaggct gccgatggcc gcgaagacga gcgacttgcc
     2161 gtcgggggac tgcgtgggcc acgagatcat ccgcacgcgg aaggtgtccg agccgatgtc
     2221 ccgcgggaag cggagcgcct tggacaccac ctgctccacg tcggccgtga acgggatctt
     2281 cgtggcagcg ccggtggtgg tgttgatgcg gaagaagccg ccctgtgcgt tgatgacgac
     2341 cgacttgccg tcgggcatga atgagaagcc ggggtagatg ccggtccagg cgaagttctc
     2401 ctgcatgtcc tggtcgaggc cgtcccagac cgggcgctcg gagcggcgat cgatgtcgta
     2461 aagataaagg accgacttca gccggtcacg gcgcacgaaa gccagcgtct tgccgtcggg
     2521 cgagagctgc ggacggccgg agccaccgta gccgtcggtg agcgtcacct ggtcgccggt
     2581 ggtcctgtcc cagcggcgga tctggtagat cccggcgaag acgttccggt tgtaggcgta
     2641 gcggctgggg cgcgcgctga agtacacgta ccgaccgtcg ggggagacga ccggttcgcc
     2701 ggcttccggc agctccccct tcaccgtcac cggcagtccg gagccgccgc gcgtgttcac
     2761 catccagagc tcggcggtgc cgatcgagct gtagtcggtg aggcgcttct tggccacgag
     2821 ccacaggccg tcggggctcc agctcgcggt gttcatcacc ttcttttcct gcgtgaccgg
     2881 gcggagatgc gtggcgtcgg cattcatcac ccagatgttg tcgcacccgc cacggtcgga
     2941 ggtgaacgac acgcgcttgc cgtcgggcga ccagcggggt tgcgaatccc aggccggtcc
     3001 cgaggtgatt cgggtggcct tcccgcccga gagggggagc gtgtagaggt cgcccatcaa
     3061 atcgaagatg agcgtccgac cgtcggggct cacgtcggac gacatccagg ttccttccga
     3121 cacggtgaag cgcaccgtgt cgctcgggcc gtgggccatg ttgacgaacc actccttcga
     3181 gtcgccgtcg tccttcttcg cggccgactt cgtggagtcg gcggcggcgc tgtcgccggg
     3241 ggcctcgccg agggagcgga gatgcgtctg cccgggcgcg ccggtcaatt cgagcgcacc
     3301 ggtcaccgaa aaggtgccga gcgaggccat ccaggcttcg gcctggtgac aggggcagga
     3361 ggcgccgccc caggcgggga cgacctcgga tcccgaggcg tccggcccgt cgaattcatc
     3421 gggctcgggg agtgcggcac gagtgccacc ggcgaggacc gcgacgagga gcgccgcgag
     3481 aaacggtcgg acaacgtgag gcgacatgac ttcccccggg aaaaatggct ggcgatcggg
     3541 cagggtcggc aggggccgac ggcggcggag tttacccggt gcgttggacg ggggggagag
     3601 caaaggcgtt cagggagatc ggggagcggg ggatgttcag ttccggcaac acggcgtcac
     3661 cgaaaccgac ccgctgggcg catagccccg gtgttgcggg ggagcgcacc cggtgtcacg
     3721 ggagcgaccc ggggtcagcg cgggcggtgg gcggagccgc ggcgcgacgg acgggagttg
     3781 gaaacccgtg cgccaccgtc ctggaaacga ttgtcccaca tcaggataac cattccgagc
     3841 accaggactg ccagttccat cgtgtcgatc gtcgacatgg ccaacctcgc tgttggagcc
     3901 gcccgccggg ctttccgggg tgggcctgac tcgtgttcgg cccactgttc agcatcttca
     3961 gtgccaaagt gtcgtcgaga cggggaaatg tcggcgagta gcccgtttgc ggcggtctca
     4021 tccggagtga tggcgccagg cgcgctcacc gcatccgatc caatcggaaa aaaattcagg
     4081 gcaaccgggt accgccgata acaacgccat cgaattcgct cgcctggcct tgttcccgaa
     4141 aggagttccg ccccgcatgc ccacccgaga ccgcttcgtg gttgcccgaa tggcgtcggg
     4201 gctcgtcctg cgcggcacca ccgtcgattt cactccggag cgtcccacgc tctggataac
     4261 tcccgacggc ggcggacgcg gcgtggaa
//
LOCUS       DASVOD010000364         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_336923, whole genome shotgun sequence.
ACCESSION   DASVOD010000364 DASVOD010000000
VERSION     DASVOD010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336923"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VF720_08390"
     CDS             complement(<1..308)
                     /locus_tag="VF720_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HEX7879413.1"
                     /translation="MMHALLEEMTHKGASDLHITAGAPPLFRVDGQIIPTDHEVVTPE
                     SAEALAYSLMKEDQRKRFEQNKEMDLSFGIKGLSRYRANIFLQRGVVTMAIRSIPYQF
                     "
     gene            complement(370..2067)
                     /gene="pilB"
                     /locus_tag="VF720_08395"
     CDS             complement(370..2067)
                     /gene="pilB"
                     /locus_tag="VF720_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX7879414.1"
                     /translation="MQDTLAKKLIETKKISSQQVAEAQKLAKGNGGNLALSLVRLGAL
                     TDAEYGDFLSSFYNTPLCDLAKADIDPTVARMVPSEVATRFQVVPIRRNGRRLTVAMA
                     NPSNIFAIDDIKFITGLEVDVMVAPEPDIKKALDRTYDQAESLASVMKDLDNDLEVVE
                     DDDEQSESVAADMAASDQAPVVKLVNSMMGDAVRKGASDIHVEPYEKQLRVRYRIDGI
                     LQEMMSPPYRMKAAIISRLKIMADLDIAERRVPQDGRIKIKVFNKMIDLRVSTLPTIF
                     GEKIVMRILDKTNLTLDLTRAGFEPKALEHMLKAISMPYGMVLVTGPTGSGKTTTLYS
                     ALSKINVPEVNVMTAEDPVEYNIEGINQVNVNEDIGLTFAAALRAFLRQDPNIVMVGE
                     IRDLETASIAVKAALTGHLVLSTLHTNDAPSTLNRMVDMGIEPFLVASSVNLIMAQRL
                     IRRVCSNCKRQVKLHDEVLSELGVPESEWMDFLDNQGAGCIECNGTGYKGRTGVFEVM
                     PISSGIRDMILDRAPTSEIKRQAALEGMLTLRMDGIRKLKLGFTSAEEVMRETALDK"
     gene            complement(2380..2952)
                     /locus_tag="VF720_08400"
     CDS             complement(2380..2952)
                     /locus_tag="VF720_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009608698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX7879415.1"
                     /translation="MRWPEFLDFTPEERRAMLAVLLFTGGGGVLIELGRRYPAWTPDF
                     RLTASLDRSRAGDSTAAGDTAAGGDSSPPGIEPDSAAGSSEHVDPAFVTAGLGRPESA
                     HEAGGKRPVADEPTGPVDLNTASAGELTRLPGVGPKLAERIVADRAAHGAYRSVAELD
                     RVKGVGPALLQKIAGMAVVSVASGAARRPG"
     gene            complement(2988..>3285)
                     /locus_tag="VF720_08405"
     CDS             complement(2988..>3285)
                     /locus_tag="VF720_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879416.1"
                     /translation="SADGWARLTRSYFRHDLAISGELRGHLIGERTNPAGEIYPTLLS
                     ADGILTAEVRALTLFLRFDNLTDLYIESDWRDPDFPYALPGVSTRLGATLRLLD"
BASE COUNT          518 a         1038 c         1126 g          603 t
ORIGIN      
        1 atgaactggt acgggatcga acggatggcc atggtgacca cgccgcgctg caggaagatg
       61 ttggcccggt agcgggacag ccccttgatg ccgaacgaca ggtccatttc cttgttctgc
      121 tcgaagcgct tccgctggtc ctccttcatc aggctgtagg cgagagcctc ggccgactcg
      181 ggagtcacga cctcgtggtc cgtggggatg atctggccat ccacgcggaa caacggcggc
      241 gccccggcgg tgatgtggag atccgaagcc cccttgtggg tcatctcctc gagaagtgcg
      301 tgcatcatga tcatcggtcg gtcctcggcg tgtgcgtgcg tgccaggaat gcggcggcgt
      361 tcgtcgtcat cacttgtcca gcgcggtctc gcgcatgact tcctcggcgg aggtgaaccc
      421 cagcttcagc ttgcgaatgc cgtccatgcg gagcgtcagc atcccttcca gggcggcctg
      481 gcgcttgatt tcgctggtcg gggcccggtc caggatcatg tcgcggatgc cggacgaaat
      541 gggcatgacc tcgaacacac cggtacgccc cttgtagccg gtgccgttgc actcgatgca
      601 gccggcaccc tggttgtcca ggaagtccat ccactccgac tctggaacgc cgagttcgct
      661 gagcacctcg tcgtgaagct tcacctgccg tttgcagttc gagcagacgc ggcggatgag
      721 tcgctgtgcc atgatcaggt tgaccgacga cgccacgagg aacggctcga tccccatgtc
      781 gaccattcga ttcagggtcg aaggagcatc gttggtatgc agcgtggaga ggaccaggtg
      841 gccggtgagg gccgccttga cggcaatgga ggccgtctcg agatcgcgga tctcgccgac
      901 catgacgatg ttcgggtcct ggcgcaggaa ggcgcgcagc gccgccgcaa aggtgaggcc
      961 gatgtcctcg ttcacgttga cctggttgat cccctcgatg ttgtactcga cggggtcctc
     1021 ggcggtcatg acgttgactt ccggcacgtt gatcttggag agggccgaat agagcgtggt
     1081 ggtcttcccc gatccggtag ggccggtgac gagcaccatg ccgtacggca tcgagatggc
     1141 cttgagcatg tgttccaggg ccttcggctc gaagccggcc cgggtcaggt cgagggtcag
     1201 gttggtcttg tccaggattc gcatcacgat cttctcgccg aagatcgtcg gaagcgtcga
     1261 aacgcgcagg tcgatcatct tgttgaagac cttgatcttg atgcggccgt cctggggcac
     1321 gcggcgctcg gcgatgtcga gatcggccat gatcttcagg cgagagatga tggcggcctt
     1381 catgcgataa ggggggctca tcatctcctg caggatgccg tcgatgcggt agcgcacgcg
     1441 gagctgcttc tcgtacggct ccacgtggat gtcgcttgcg cccttccgga cggcatcgcc
     1501 catcatcgag ttcacgagct tgaccaccgg cgcctggtcc gaagccgcca tgtcggcggc
     1561 caccgactcc gactgctcgt cgtcgtcctc cacgacctcc aggtcgttgt cgaggtcctt
     1621 catgacgctg gccaggcttt ccgcctggtc gtaggtgcga tcgagagcct tcttgatgtc
     1681 gggctcggga gccaccatca cgtcgacttc gaggccggtg atgaacttga tgtcgtcgat
     1741 ggcgaagatg ttcgagggat tggccatcgc cacggtcagg cgccggccgt tgcgacggat
     1801 cggtaccacc tggaagcgcg tggccacctc ggacggcacc atccgggcca ccgtcgggtc
     1861 gatgtcggcc ttcgccaggt cgcaaagcgg cgtgttgtag aaactcgaga ggaagtcgcc
     1921 gtactccgcg tcggtcagcg cgccgaggcg caccagcgac agggccaggt tgccgccgtt
     1981 tcccttggcg agcttctggg cttcggccac ctgctggctc gagatcttct tcgtctcgat
     2041 cagtttcttg gcgagggtgt cctgcaagga aaagcctcct ggtcgagcct gacctggatt
     2101 cgttgacgcg cgattcgtct cgtcccgccg gcgggtttgc attcctcccg atccggggca
     2161 cgaagccact ccgaccaccg gggccgggcg ttccggataa atcggcatgc gggcgggaga
     2221 tattgaggaa agtcttgcgc gtggcggccc gagccacgcc ttctgcacgg atggggacgc
     2281 cttctgcacg tcgatcggcc gccgggccca gggttgcgaa cgccccgccg gccgcccagg
     2341 ctccccggac ccagccgtgc cggccccgtg ggaagccgct catcccggcc ttcgtgcggc
     2401 acctgaagcc acgctcacaa ccgccatgcc ggcaatcttt tgcagcaatg cagggcccac
     2461 tcccttcacc cggtcgagct cggccaccga gcggtaggct ccatgcgccg cccggtcggc
     2521 cacaatccgt tcggcgagct tcgggcccac gcccggaagg cgtgtcaatt cccccgccga
     2581 agccgtgttg aggtcgacgg ggccggtcgg ttcgtcggcc acggggcgtt ttcccccggc
     2641 ttcatgagcc gattccgggc gaccgaggcc ggccgtcacg aaggcggggt ccacatgctc
     2701 cgacgacccc gcggccgagt cgggctcgat cccgggggga gaggagtcac cgccggcggc
     2761 ggtgtcccct gccgccgtgc tgtctcccgc gcgcgatcga tcgagggagg ccgtcagccg
     2821 gaaatcaggg gtccatgccg ggtagcggcg gccgagctcg atgagcacgc ctccgccgcc
     2881 ggtgaagagc aggaccgcca gcatggcgcg gcgttcttcg ggagtgaagt ccaggaattc
     2941 gggccagcgc atgagagtcc tccggacggg aggcctggga tgcgggctta gtcgagcagg
     3001 cgcaacgtgg cgccgagccg ggtcgacaca ccgggaagcg cgtaggggaa atcggggtcg
     3061 cgccagtcgg actcgatgta gagatcggtg aggttgtcga accggaggaa gagcgtgagg
     3121 gcgcggacct cggccgtcaa gatgccgtcg gccgacagga gcgtgggata gatctcgccg
     3181 gccggattgg tccgctcgcc gatcaggtgc ccgcgcagct cgccggagat cgccaggtca
     3241 tggcggaagt agctccgggt gaggcgcgcc cacccgtcgg cgctg
//
LOCUS       DASVOD010000365         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_26777, whole genome shotgun sequence.
ACCESSION   DASVOD010000365 DASVOD010000000
VERSION     DASVOD010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26777"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VF720_08410"
     CDS             <1..287
                     /locus_tag="VF720_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7879417.1"
                     /translation="PTVVLYEVVTWARRRETPSRLNEVTALLSRANLIPLSESIAIEA
                     SGIAHSSKLAMADSIILATARMTGSELWTQDADFRDFPDVRYIPRTQGKP"
     gene            284..2017
                     /locus_tag="VF720_08415"
     CDS             284..2017
                     /locus_tag="VF720_08415"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7879418.1"
                     /translation="MTPNTTPERLGYAKGLFTGHIDESAIFPYPAMPAEEAETVEALL
                     EPVERFLDEKVDSAAIDHAGELPESIIRGIAELGLMGLIVPEAYGGLGLSMTAYARVM
                     ETIAGRDASVAIHIGCHQSIGMKALLLHGSEDQKKKWLPRAATGEVVCAYALTEPGSG
                     SDAAAMKTTGRHDPARGVWVLNGVKQWITNGGYADLVTVFARTPVERDGKTEEKTTAF
                     LVERSLPGFSSGKPEKKLGIRASSTTDIILEDCPVPEFNVVGEVGRGFKVAMEVLNTG
                     RLTLATGSIGGSKSMLKVALAHAEQRKAFGRPIIEYEMIEEKFAEMAVHIYAMEAMTY
                     LTTGLIDRGATDYAVESAMCKVFCSERLWENVNGALQVAAGNGYMAEYPYERYLRDAR
                     INLIFEGTNEILRLFIALSGLQRPGEYLKEVGKALHAPIKSFGLLRDFAGIHLQRTVK
                     PPVLRMVAQPLARDAERLSRLTMSFADQCEAVLRQHGKSIVEREYLQKRLSDAATDLY
                     AVSACLSRATAAIESSGEAGAADHVRLAHTATNLAVRRIRRNLRMVEKNSDARYHEVV
                     GWLRERGEYRL"
BASE COUNT          375 a          707 c          700 g          329 t
ORIGIN      
        1 tgccgaccgt cgtcctctac gaagtggtca cgtgggcgcg ccggcgcgaa acgccctccc
       61 ggctgaacga ggtcacggcc ctgctgagcc gcgccaacct gattccattg tcggagagca
      121 tcgccatcga ggccagcggc atcgctcact cctcgaaatt ggcgatggcg gacagcatca
      181 tcctggccac cgccaggatg accggctcgg aattgtggac acaggatgcg gacttccggg
      241 atttccccga tgtccgttac atcccacgca cccagggcaa accatgacgc cgaacaccac
      301 ccccgaacgc ctcggctacg ccaaggggct cttcaccggc cacatcgacg aatccgccat
      361 ctttccgtac cccgccatgc cggccgagga agccgaaacc gttgaggcgc tcctcgaacc
      421 ggtcgagcgt ttcctcgacg agaaggtcga ttcggccgcc atcgaccacg cgggcgagct
      481 gcccgaatcg atcatccgcg gcatcgccga gctggggctc atggggctca tcgtgccgga
      541 ggcgtacggc gggctcgggc tgtcgatgac cgcgtacgca cgcgtcatgg agacgatcgc
      601 ggggcgcgat gcctcggtgg ccatccacat cggctgtcac cagtcgatcg ggatgaaggc
      661 gctcctgctg cacggcagcg aagatcagaa gaagaagtgg ctgccgcgcg ccgccaccgg
      721 tgaggtcgtg tgcgcctacg cgctcaccga gccgggctcg ggttcggatg ccgccgccat
      781 gaagaccacc gggcgtcacg acccggcgcg cggcgtgtgg gtgctgaacg gcgtcaagca
      841 gtggatcacc aacggcggct acgccgacct tgtcacggtc tttgcccgaa cgccggtgga
      901 gcgggatgga aagaccgagg agaagaccac tgccttcctc gtcgaacgct cccttcccgg
      961 attctcgagc ggcaagccgg agaagaagct cggcatccgc gcctcttcca ccaccgacat
     1021 catcctcgag gattgcccgg tgccggagtt caatgtggtc ggcgaggtgg ggcgcggctt
     1081 caaggtggcc atggaggtgc tcaatacggg gcgcctcacg ctggccaccg ggtcgatcgg
     1141 cggcagcaag agcatgctca aggtggcgct ggcgcacgcg gaacagcgaa aggcgttcgg
     1201 ccggcccatc atcgagtacg agatgatcga ggaaaagttc gccgagatgg ccgtccacat
     1261 ttacgccatg gaggcgatga cctacctcac caccggcctc atcgaccggg gtgccacgga
     1321 ctatgcggtg gagtcggcca tgtgcaaggt gttctgctcg gagcgcctct gggaaaacgt
     1381 gaacggggcg cttcaggtgg cggcggggaa tggctacatg gccgaatatc cgtacgagcg
     1441 ttacctgcgc gacgcccgca tcaacctcat cttcgagggg acgaacgaga tcctccgcct
     1501 cttcatcgca ctctcgggac tgcagcggcc gggcgagtat ctcaaggagg tggggaaggc
     1561 gttgcatgcg cccatcaagt cgttcggcct cctgcgcgat ttcgccggga tccatctgca
     1621 gcggacggtc aagccgccgg tgctcaggat ggtggcgcag ccattggccc gcgatgccga
     1681 acgcctctcc cggctcacca tgtcgttcgc cgaccagtgc gaggcggtgc tccggcagca
     1741 cggcaagtcg atcgtggagc gggagtatct ccagaagagg ctgtccgacg cggccaccga
     1801 cctctatgcc gtgtcggcct gcctgtcgcg cgccacggcc gccatcgaat cgtcgggtga
     1861 agccggcgcc gccgaccatg ttcgcctggc ccacaccgcc accaacctgg cggtgcgccg
     1921 catccggcgc aacctgcgca tggtcgaaaa gaactcggac gcccgctacc acgaggtggt
     1981 ggggtggctc cgcgagcggg gcgagtaccg gctgtagccg gccccgacag gtggtggcgc
     2041 gctacgtgcg gatgggagct cgcgcccgcc tcttcgccca ccagcgcacg acgaggatcg
     2101 gcacgcccac g
//
LOCUS       DASVOD010000366         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_26893, whole genome shotgun sequence.
ACCESSION   DASVOD010000366 DASVOD010000000
VERSION     DASVOD010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26893"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /gene="coxB"
                     /locus_tag="VF720_08420"
     CDS             complement(<1..625)
                     /gene="coxB"
                     /locus_tag="VF720_08420"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX7879419.1"
                     /translation="MTTTGNKTLPLFPEQASTHAASVDNLYFYLLGLAAFFTLLVIAL
                     VVFFAIKYRRRSESDLPPRIHGSMALEMLWTIIPLGLSLVTFYLGAKVYFDLSRPPDN
                     AMEIWVVGKQWMWKVQHLEGVREINELHVPVGRPVKLTLTSEDVIHSFYVPAFRIKQD
                     AVPGRYTTTWFEATKVGSYHLFCAEYCGTVHSGMIGTVVVMDPADYQA"
     gene            complement(622..1572)
                     /locus_tag="VF720_08425"
     CDS             complement(622..1572)
                     /locus_tag="VF720_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX7879420.1"
                     /translation="MSLRIHRRSGVLGAALAASLPFLALSTTSLPAFGQGGGAPRFSG
                     GMHGGFTGADDENPSYNDGRPAALEGVGVDQRLDNPVPLDLEFVDETGRTVRLGDYFE
                     AKKPVILTLAYYGCPMLCTQVLNGEAESLKALGLTPGKDFEVVTVSFDSTETPALAHA
                     KRSNYIDRVDRPGIENTWHFLTGKSASIAVLTDAVGFRYKWDEETEQFAHSSVLMVLT
                     PEGHVSRYLFGMEFAPRDLRFALIEASENRIGNVVDQVLLFCYHYDPATGQYGAAIMN
                     ILRLAAVLTFVGIVGLVLGLLRRERAGHGSPFMPRPAGGR"
     gene            complement(1569..2123)
                     /locus_tag="VF720_08430"
     CDS             complement(1569..2123)
                     /locus_tag="VF720_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879421.1"
                     /translation="MSVDAHKPGPPASKEALAAGHEISEIQPKPILAFLILIAIFSAF
                     CFGIIVVYFRFLAADAAKHSPRPSPLITQGSARVPPEPRLQVDAWGDWDSYKADQDRQ
                     LGHYSWANKDSLTIRMPIERAMEIVLQRGLPARAGTMPPPSPDGGEAPGHEAGGRMAP
                     HDDLRMKPPTSRTEPAPTGAGGHR"
     gene            complement(2113..>2380)
                     /locus_tag="VF720_08435"
     CDS             complement(2113..>2380)
                     /locus_tag="VF720_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879422.1"
                     /translation="LINRYVKRNPRSLQRVALLIIVMRLVEIFFSVAPAHEDIRPFAV
                     HWLDLAVPVGLTGLWLAAFLNELKGRTAVPGINHLSEEKAPHVG"
BASE COUNT          419 a          754 c          837 g          370 t
ORIGIN      
        1 aggcctgata gtcggctggg tccatgacca cgaccgtacc gatcatcccc gagtgcaccg
       61 tgccgcagta ctcggcgcag aagaggtggt agctcccgac cttcgtcgcc tcgaaccacg
      121 tggtcgtgta gcggccgggg acggcgtcct gcttgatgcg gaacgcgggc acgtagaacg
      181 aatggatgac gtcctcggag gtgagcgtca gcttcaccgg acggccgacc ggaacgtgca
      241 gctcgttgat ctcgcgcaca ccctccaggt gctggacctt ccacatccac tgcttgccga
      301 cgacccagat ctccatggcg ttgtcgggcg ggcgcgagag gtcgaagtag accttggcgc
      361 cgaggtagaa cgtgaccaga gagagcccga gcggaatgat cgtccagagc atctcgagcg
      421 ccatcgagcc gtggatgcgc ggcgggaggt cgctctccga gcgccggcgg tacttgatgg
      481 cgaagaagac gaccagcgcg atgaccagca gcgtgaagaa cgccgccagt ccgagcaggt
      541 agaagtagag gttgtcgacc gaggcggcat gcgtggacgc ctgctcgggg aacaacggga
      601 gcgtcttgtt gccggtcgtg gtcatcggcc acccgccggc cgcggcatga agggcgagcc
      661 atggcccgcg cgttcccgcc gcagcaatcc gaggacgagg ccgacaatgc cgacaaacgt
      721 gagcacggcc gccagccgga ggatgttcat gatcgcggca ccgtactggc cggtggccgg
      781 atcgtagtga tagcagaaga ggaggacttg gtcgaccacg ttgccgatcc ggttctcgga
      841 agcttcgatg agtgcgaagc gcaggtcacg cggggcaaac tccatcccga agagataacg
      901 ggagacatgc ccctccggcg tcaggaccat gagcacgctg gagtgggcga actgctcggt
      961 ttcctcgtcc cacttgtagc ggaagccgac ggcgtcggtc agcaccgcga tcgacgcgga
     1021 cttgccggtc aggaagtgcc aggtgttctc gatgccgggg cggtcgacgc ggtcgatgta
     1081 gttggaccgc ttggcatggg cgagggccgg agtctcagtc gagtcgaagc tcaccgtgac
     1141 cacctcgaaa tccttcccgg gggtgaggcc aagcgccttc aggctttccg cctcgccgtt
     1201 cagaacctgc gtgcagagca tcgggcagcc gtagtaggcc agggtcagga tcaccggctt
     1261 cttcgcttcg aagtagtcgc cgaggcgcac cgtcctgccg gtttcatcca cgaactcgag
     1321 gtcgagcgga acgggattgt cgagacgctg atcgacgccc acgccctcga gcgccgccgg
     1381 gcggccatcg ttgtacgagg gattttcgtc gtcggcgccg gtgaacccac catgcatgcc
     1441 gcccgagaag cgcggtgcac cacccccctg cccgaaggcg gggagtgacg tcgtcgagag
     1501 ggcaaggaac gggagcgacg cggccagtgc ggccccgagg acgccggaac ggcgatggat
     1561 gcggaggctc accgatgccc ccccgcaccc gtcggcgccg gttcggtccg gctggtcggt
     1621 ggcttcatcc ggaggtcgtc gtggggggcc attcggccac ccgcctcgtg ccccggagcc
     1681 tctcccccgt ccggggacgg gggcggcatg gtgccggcgc gagccggaag gccgcgctgg
     1741 agcacgattt ccatggcacg ctcgatcggc atccgaatgg tcaggctgtc tttgttggcc
     1801 caggaatagt gcccgagctg ccggtcctgg tcggccttgt agctgtccca gtcgccccag
     1861 gcatccacct ggagccgcgg ctcgggcggc acacgggccg aaccctgcgt gatgagcggg
     1921 ctcggccgcg ggctgtgctt cgccgcgtcg gccgccagga agcggaagta aacgacgatg
     1981 atcccgaagc agaaggccga gaagatggcg atcaggatga ggaaagccag aatcggcttc
     2041 ggctggatct cgctgatctc gtggccggcg gccagcgctt ccttcgaggc cggcggaccc
     2101 ggcttgtgcg cgtcaaccga catgcggcgc cttctcttcc gacaggtgat tgatccccgg
     2161 gacggcagtg cgccccttca gctcgttgag gaacgcggcc agccagaggc ccgtcaggcc
     2221 gaccggcacc gccaggtcga gccagtgcac ggcgaacggc cggatgtcct cgtgggccgg
     2281 cgccacgctg aaaaagatct cgaccaggcg catgacgatg atgaggagcg ccacccgctg
     2341 cagggagcgc ggattccgct tcacgtagcg gttgatcagc
//
LOCUS       DASVOD010000367         5518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_443305, whole genome shotgun sequence.
ACCESSION   DASVOD010000367 DASVOD010000000
VERSION     DASVOD010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443305"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF720_08440"
     CDS             <1..320
                     /locus_tag="VF720_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879423.1"
                     /translation="PDPSKFPCLELAYRAGRAGGVMPAAMNAADEVAVAAFLDGKIGF
                     MDIPAVIEQVMEAVGTDTQSAVASGAASTAIPSIEAVLAADLAAREAAHDIVARCTPP
                     VAR"
     gene            322..1404
                     /locus_tag="VF720_08445"
     CDS             322..1404
                     /locus_tag="VF720_08445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7879424.1"
                     /translation="MRIAHVDSGRSWRGGQAQVAYLLEGLLAAGHRVMLAAPKGAPLA
                     ERGRSLGIEVIDFRPWGDLDAGATLGLGAAVKRFGADIVHGHSAGAHLGAAMAGRLAR
                     APVVVTRRLDLPIGRSGFSRWKYRNGVRRYVAISQCVARSLEAGGIEPGSIRVVYSGV
                     PVGGPRAADPAARSAARRSLGLNDTEVAAVLVGAMTEQKGHDLAVTAMAQVTAPLKLV
                     FLGDGPDRAALEKQVAALDLGERIVFAGFRKDVPALLPAFDFLVAPSRHEGLGTSVLE
                     GMAAGLPVVATRVAEFEDVLDQGKAGRLVGAGDVPALALAMNELVGDGALRARLGLAS
                     LERAGRYAVTEMVQGNIRVYDELRRG"
     gene            1421..2866
                     /gene="rseP"
                     /locus_tag="VF720_08450"
     CDS             1421..2866
                     /gene="rseP"
                     /locus_tag="VF720_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEX7879425.1"
                     /translation="MFKLILFGVPALGLVVLVHELGHFLMARLCRVPVDKFSIGFGPR
                     LLGWTSGRTEYALSAIPLGGYVKMAGEEALEEGVTPGPDTFLGHPWWHRVLIALAGPG
                     ANFVFAFVVLTLLYLTGVSYPDSSNVVGRVTPGSTIAALDLRPGDVVVEVAGKPTTRG
                     GEVRLGLAGGEQVPHRKNPVDMAMVVERNGARVPVAVSAGQLNAVIDSLLFYTPAIIG
                     DVVSGTPAYVAGLRNGDTILSIEGEPVGEWIDVLERISARPDQPTRLVVRRDGKEINL
                     SVTPLRQEENGRDVGRIGVVVQSREYKASYPIGTAIQYGAAQTVGLSGAQFAAIGGLV
                     MNPKAIGRSMSGPLAIMEMTGQAAERGVDELFRLVALISIALMAFNLLPIPVLDGGLV
                     VMALAEGIRRRPVPIKVQVVFQQIGLVMLGSLILFVLFNDPMKMIRRNRALSQQQKSA
                     PAATSPAHDEAPAHDETPAPAADSGSSGSGH"
     gene            2811..4850
                     /locus_tag="VF720_08455"
     CDS             2811..4850
                     /locus_tag="VF720_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX7879426.1"
                     /translation="MTRRPRRPPTRVRADRGTDIRHRRSRALRTALAGAALLAWLVPG
                     GVGGAAYAADDLELLPKAARIRKVVVEGAQTLNAGDLKKSLSIKMPDWLHPFRKPRFY
                     RGLVQQEARTMESFARQKGLAAATVSWDTTSVEGGVSLTFRIEEGPAYQVAGLDLSGV
                     DAETDNRMRNAVSLEPGKPWTVTGVEADRAVIDNDLADRGHPFSRIRTYAEVGHDSVL
                     IVHDISPGPVTKIDGISVAGGEGLSVSGLEREITVDGGDMYSRREIERSRQRLYDTGF
                     FNDVSVAYPPAGIDSVNNTVDVQFNLVQRKQKWMSGGVGYTSDNLLRLTAEWGTRSIF
                     GTGRGFKINARTAREIFGDVPFFTERQSLAQATLSERWLFGTRIHGSLNAYYTFDNLR
                     NEFVKQDIVGAGAEASYEMKERRNSVALLFDARRTSSDVEPDSLAEEVCEDDPSFCRS
                     RYITRSVVGRLTLDERNDFLNPTRGYRTELQLESAGGILGGDSNFLKGSTGTSWLRPM
                     GKTGTVGVRLRFGAIEPDSGLVPGEMGEQAVDDITSVPFEERFISGGANTVRGYQENT
                     LNGLADGKDPSGGGLFEILANVEMRFPIRGPLGGVLFVDAGNVWREYGKISLKDFAPT
                     FDIDETTPEHLRWSVGGGIRFNTPVGPVRLEGVYRLSPARRDYPSDIHLAVGQVF"
     gene            4850..>5518
                     /locus_tag="VF720_08460"
     CDS             4850..>5518
                     /locus_tag="VF720_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879427.1"
                     /translation="MWWRRLRVILRRLGVGVFLGLLLTIIALVVGWLMAGDWVAGRLA
                     SYANNKFFTDRNTRLTVGRVTGSVWSDMVFERVRIEREIDGHWLSGLWAEQVEARYDL
                     IGLIRGRPDFSSVRVFSPVIELSADSTGRIVLPIGTRPGERGGGEGLKSLTMHDIAVQ
                     DGVFRFSGRLRGFEANHLFAQASLEIKGSGVDLNLEQASMELLDPIGRIEKVSGRVTV
                     AGRTI"
BASE COUNT          835 a         1818 c         2003 g          862 t
ORIGIN      
        1 cgccggatcc ttcgaagttt ccctgcctcg aactggccta ccgggcgggt cgggccggtg
       61 gcgtcatgcc ggccgccatg aacgccgcgg atgaagtggc ggtggcggcc ttcctcgacg
      121 ggaagatcgg cttcatggac atcccggccg tgatcgagca ggtgatggag gcggttggga
      181 cggataccca gagcgcggtg gcgtccggcg cggcgtcgac ggccatccct tccatcgagg
      241 ccgtgctcgc cgccgacctc gccgcccgcg aagcggcaca cgacatcgtg gcccgctgca
      301 cgccgccggt ggcgcggtga cgtgcggatc gcccatgtcg attcgggacg ctcgtggcgc
      361 gggggacagg cgcaggtggc ctacctcctc gaaggcctgc tggccgccgg tcatcgggtg
      421 atgctggccg cgccgaaagg ggcgccgctg gccgagcggg ggcggagcct cggcatcgag
      481 gtgatcgact tccgaccgtg gggagacctg gacgcgggcg ccacgctggg actcggggcc
      541 gcggtcaagc gcttcggggc cgacatcgtg cacggccatt cggccggtgc gcatctcggg
      601 gcggccatgg ccgggcggct ggcgcgtgcc ccggtggtcg tgacgcggcg gctcgacctg
      661 ccgatcgggc ggagcggttt ctcgcgctgg aaataccgga acggcgtgag gcgctatgtg
      721 gccatctcgc agtgcgtggc cagaagcctc gaagccggcg gaatcgaacc cggctcgatc
      781 cgggtggttt acagcggggt tccggtcggc ggcccccggg ctgccgatcc ggccgcccgg
      841 agcgcggccc gccggtcgct cggcctcaac gataccgagg tggccgccgt cctcgtcggg
      901 gccatgaccg agcagaaagg tcacgacctg gccgttaccg cgatggcgca ggtcaccgcc
      961 ccgctcaagc tggttttcct cggagacggt ccggatcggg ccgccctcga gaaacaggtg
     1021 gccgcgctcg acctcggcga acggatcgtc ttcgccggat tccggaagga cgtgccggcg
     1081 ctcctgccgg cgttcgactt cctggtggcg ccgtcgcgtc acgaggggtt gggtacgagc
     1141 gtcctcgagg ggatggcggc cggcctcccc gtcgtggcca cgcgggtggc cgagttcgag
     1201 gatgtgctcg accagggaaa agccggccgg ctggtgggcg cgggagacgt gcccgcgctg
     1261 gccctggcaa tgaacgaact ggtcggggac ggcgcacttc gcgcccgcct cggcctggcc
     1321 tcgctcgaac gggcggggcg ctatgcggta acggaaatgg ttcaaggcaa catccgggtg
     1381 tatgacgaat tgaggcgggg atgaaccgcc gggagagatg atgttcaagc tgatcctgtt
     1441 cggtgttccc gccctcggcc tggtcgtgct ggtgcacgag ctcgggcact tcctgatggc
     1501 ccggttgtgc cgggtgccgg tggacaagtt ttccatcggc ttcggcccgc gcctgttggg
     1561 atggaccagc gggcgcaccg agtacgccct ctccgccatc ccgctgggag gctacgtgaa
     1621 gatggccggc gaggaggcgc tggaagaggg ggtcacgccg gggcccgata ccttcctcgg
     1681 ccacccctgg tggcaccggg tgctcattgc gctggccggc ccgggcgcca atttcgtctt
     1741 cgcgttcgtg gtgctcacgc tcctttatct caccggcgtc agctatcccg actcgtccaa
     1801 cgtggtggga cgcgtgacgc cgggctccac gatcgcggcg ctcgatctgc gccccggcga
     1861 cgtggtggtg gaggtggccg gcaagcccac cacccgcggc ggcgaagtgc ggctggggct
     1921 ggcgggcggc gagcaggtgc cgcatcgcaa gaaccccgtg gacatggcga tggtcgtcga
     1981 gcgcaacggc gcgcgcgtgc cggtggcggt atcggccggc cagctcaacg cggtgatcga
     2041 ttcgctcctc ttctacacgc cggccatcat cggcgacgtg gtgagcggca cgcccgccta
     2101 tgtggccggg ctgcgcaacg gcgatacgat cctttcgatc gagggtgagc ccgtcggcga
     2161 gtggatcgac gtgctcgaac ggatctcggc ccgccccgac caaccgacgc ggctggtggt
     2221 gcggcgcgac gggaaggaaa tcaacctctc ggtgacgccg ctccgccagg aagagaacgg
     2281 cagggatgtg ggacggatcg gcgtggtcgt gcagagccgc gagtacaagg ccagctatcc
     2341 gatcggcacc gccatccagt acggcgcggc gcagacggtg gggctctccg gggcgcaatt
     2401 cgcggccatc ggcggcctgg tcatgaatcc caaggcgatc ggccgctcga tgtcggggcc
     2461 gctggccatc atggaaatga ccgggcaggc ggccgagcga ggcgtggacg agctcttccg
     2521 actggtggcg ctgatctcga tcgcgctcat ggccttcaac ctgctgccga tcccggtgct
     2581 cgacggcggg ctcgtggtca tggcactggc cgaggggatc cgccgacggc cggtgccgat
     2641 caaggtgcag gtggtgttcc agcagatcgg cctcgtgatg ctcggctcgc tcatcctctt
     2701 cgtgctcttc aacgacccga tgaagatgat ccgccggaac cgggcgcttt cgcagcagca
     2761 gaagtcggcg cccgcggcca cgtctcccgc gcacgacgag gcgccggcgc atgacgagac
     2821 gcccgcgccg gccgccgact cgggttcgag cggatcgggg cactgacatc cggcaccgtc
     2881 gttcccgcgc ccttcggacc gcgctcgccg gagccgcgct cctggcgtgg ctcgtgcccg
     2941 gcggagtggg aggggcggcg tacgccgccg acgatctcga gttgttgccc aaggccgcac
     3001 ggatcaggaa agtggtcgtg gaaggagcgc agacgctcaa cgccggtgac ctgaaaaaga
     3061 gcctgtcgat caagatgccc gactggctcc acccgttccg gaagccccga ttctaccggg
     3121 gcctcgtgca gcaggaagcg cgcacgatgg agtcgttcgc ccggcagaag gggctggcgg
     3181 ccgccacggt gagctgggac acgacctccg tggaaggggg ggtgagcctt actttccgga
     3241 tcgaggaggg gccggcttac caggtggccg gcctcgacct ttccggtgtc gatgccgaaa
     3301 ccgacaaccg gatgcgcaac gccgtgtcgc tggagccggg caaaccctgg acggtgacgg
     3361 gcgtggaggc cgaccgggcc gtcatcgaca acgacctggc cgatcgcggc caccccttct
     3421 cccgcatccg tacgtatgcc gaggtggggc acgacagcgt gctcatcgtg cacgacatct
     3481 ccccgggacc ggtcacgaaa atcgacggca tctcggtggc ggggggtgaa ggcctgagcg
     3541 tcagcgggct cgagcgcgaa atcaccgtcg acggcggcga catgtacagc cgcagggaga
     3601 tcgagcgaag ccgccagcgc ctgtacgaca ccggcttctt caacgacgtg tcggtggctt
     3661 acccgccggc cggcatcgat tccgtgaaca acacggtgga cgtgcagttc aacctcgtcc
     3721 agcgcaagca gaagtggatg tcgggcggcg tcggttacac cagcgacaat cttctccggc
     3781 tcacggccga gtggggcacc cgcagcatct tcggcacggg gcgcggcttc aagatcaacg
     3841 cccgcaccgc ccgcgagatc ttcggcgacg tgcccttctt caccgagcgg cagtcgctgg
     3901 cgcaggccac gctgagcgag cggtggctct tcggcacgcg catccacggt tcactgaacg
     3961 cctactacac gttcgacaac ctccggaacg agttcgtgaa gcaggatatc gtgggcgccg
     4021 gcgccgaggc cagctacgag atgaaggagc ggcgcaactc ggtggcgctg ctgttcgacg
     4081 cgcgccgcac ctcgagcgac gtggaacccg acagcctggc cgaggaggtc tgcgaggacg
     4141 accccagctt ctgccggagc cgctacatca cccgttcggt ggtggggcgc ctcacgctgg
     4201 atgagcggaa cgacttcctg aaccccacgc gcggctatcg caccgagctg cagctcgaaa
     4261 gcgccggcgg catcctcggt ggcgacagca acttcctgaa ggggagcacc ggaacgtcgt
     4321 ggctcaggcc gatgggaaag accggcaccg tcggcgtgcg gctccgcttc ggcgccatcg
     4381 agcccgactc gggtctcgtg ccgggcgaga tgggcgagca ggcggtggac gacatcacca
     4441 gcgtgccgtt cgaagagcgc ttcatctccg gcggggccaa cacggtgcgc ggctaccagg
     4501 aaaacacgct caacggcctc gccgacggca aggacccgtc gggcggcggc ctgttcgaga
     4561 tcctggccaa tgtcgagatg cgattcccga tccgcggccc cctgggcggg gtgctcttcg
     4621 tggatgccgg gaacgtctgg cgcgagtacg gaaagatcag cctcaaggac ttcgcgccga
     4681 cgttcgacat cgacgagacg acgcccgagc accttcgctg gtcggtgggg ggcggcatcc
     4741 gcttcaacac gccggtggga ccggtgcgcc tggagggggt gtaccgcctc tcgcccgccc
     4801 gccgcgatta cccgagcgac atccacctgg cggtcgggca ggtgttctga tgtggtggcg
     4861 acgtctgcgc gtcatcctgc gccggctcgg cgtgggtgtg tttctcgggc tcctcctcac
     4921 gatcatcgcg ctggtggtcg gctggctcat ggccggcgac tgggtggcgg ggcggctcgc
     4981 gagctacgcc aacaacaagt tcttcaccga ccgcaacacg cgcctcaccg tgggccgggt
     5041 gaccggctcg gtctggagcg acatggtgtt cgagcgcgtc cgcatcgagc gcgagatcga
     5101 cggccactgg ctctccggcc tctgggccga gcaggtggag gcccgctacg acctgatcgg
     5161 gctgatccgc gggcgcccgg acttctcgtc ggtgcgcgtc ttttcgccgg tcatcgagct
     5221 gtcggccgac tccaccggcc gtatcgtcct cccgatcggc acgcgccccg gggagcgtgg
     5281 cggtggcgag gggctgaaaa gcctcacgat gcacgacatc gcggtgcagg acggcgtctt
     5341 ccggttcagc ggccgattgc gcggcttcga ggccaaccat ctctttgcgc aggcctcgct
     5401 cgagatcaag gggagcggcg tcgacctgaa cctcgaacag gcttcgatgg agctgctcga
     5461 cccgatcggc aggatcgaga aggtgtcggg gcgcgtgacc gtggccgggc gcaccatc
//
LOCUS       DASVOD010000368         9603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_337372, whole genome shotgun sequence.
ACCESSION   DASVOD010000368 DASVOD010000000
VERSION     DASVOD010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337372"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..377
                     /locus_tag="VF720_08465"
     CDS             114..377
                     /locus_tag="VF720_08465"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7879428.1"
                     /translation="MMLARVIGTVVSTVQHASLNGRRLLLARRLGLDGKPTGSTLIAV
                     DTVQATPGQTVLILDEGNGARQVLGAPEAPIRTVIVGVVDRAG"
     gene            complement(393..1166)
                     /locus_tag="VF720_08470"
     CDS             complement(393..1166)
                     /locus_tag="VF720_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879429.1"
                     /translation="MAPRKITHRILIATLLAACAHAMPAASNTAYQSIPFFQNWSNTA
                     LLSTSDNWNNVPGIVGYLGENPIESTPGRLPRTITADSTSAPDLIPNQGDPSGIIIGG
                     VAECQLTDPVVALQASATADHPYLVIHLNTTGIPAPLVFQCRLRDLDGSLDNTQQPIQ
                     VQYRTQASGSWTNAVGGDFFDVTHGPGLNTLQTMVSIVLPGVVGNRPQLQVRIMTANA
                     VGNDEWVGIDDIYVTVATSGVPAPGSLEPTTWSRIKNLR"
     gene            complement(1238..1978)
                     /locus_tag="VF720_08475"
     CDS             complement(1238..1978)
                     /locus_tag="VF720_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879430.1"
                     /translation="MKAKVLTLVAVALIGAAGNAFATGTPQGLPFSQDWTNIGLITAN
                     DDWSGVPGILGFLGDNPVTSVTGVNPTSLVGDSSGVQDVIANGTNPNTSTAGGVGEFH
                     ITNPVVALQGSGTADFPHLRFHVVTTGLQSITFNCNLRDIDGSTDNANTPVAVQYRVG
                     NAGNWTNVTSVADATTGPSLATLVTPVSAVLPAGANNQPEVQIRVLTTNSVGNDEWIG
                     IDDISITGTNLPVPTETSSWSAIKKGSN"
     gene            2323..2871
                     /gene="hpt"
                     /locus_tag="VF720_08480"
     CDS             2323..2871
                     /gene="hpt"
                     /locus_tag="VF720_08480"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007932290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX7879431.1"
                     /translation="MHDDIDRILYTDDDIQNRIRALARDINEAYPTGDLVLLGVLSGS
                     LLFLADLARALDRHVIVDFIAVSSYEGGTQSTGVVRLLKDLSVNIEGKDVLLVEDIID
                     TGLTINYLIENLTTRHPRSLAVCALLDKAEARKAPVPIRFTGFTVPNEFLVGYGLDYQ
                     QRYRNLPYVGILKPRVFQPAGS"
     gene            2868..4268
                     /locus_tag="VF720_08485"
     CDS             2868..4268
                     /locus_tag="VF720_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7879432.1"
                     /translation="MTTPHGAPRIPGGGLLFRNGRWITMDAARRVVDGDCYVVDGRIE
                     HLGGRFEAPEATPVTDLSGRWVTPGFIQGHVHLGQTLFRGLGENRRLLDWLKDVIWPL
                     EAAHDDDSAYWSAVVGGIEAMRAGTTAMLDIGLVHGAAGLFKGFLDLGIRGWTGKLMM
                     DRGDGVPKGLLADPNHILAESHELARKWDGVDNGRLRYAVCPRFVFSCSPALWERAVA
                     EAKGGDWPIHTHAFETREEQEAVRAIEHRSELDTLADVGALGASLKVAHGIWVDDPTA
                     ARLGGASASVIHCPASNLKLGSGVADLTRLKAHGVKVGIGCDGSPCNNFLDPLGEMRL
                     AALLQKWKHGPEAYGAADALALATCDGARALDAEDRIGSIEVGKRADFVVFDLERVHS
                     IAADAVDPYTRIVFGADRSNIESVYVEGRAVVERGRVPGFDEAEVVTKGKESIAAVLA
                     RAGRFGSGTGPVKMAP"
     gene            4333..4998
                     /gene="udk"
                     /locus_tag="VF720_08490"
     CDS             4333..4998
                     /gene="udk"
                     /locus_tag="VF720_08490"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX7879433.1"
                     /translation="MNPPACWIIGIAGGSASGKTRVAHAIVEELGPDEVQVIDQDAYY
                     RDLAHLPMEDRKRQNFDHPSAFDTELMLEHLTVLRQGGWVDKPVYDYAAHTRSSRTER
                     VGGHAVLVLEGILVLDDPRLRALMSVKVFIDTDPDVRLIRRLRRDVTERGRSVESVLQ
                     QYEASVRPMHQQFVEPSKRHADIIIPEGVENHVGVDILRTKVHALANASARNARADLT
                     PSR"
     gene            5027..5851
                     /locus_tag="VF720_08495"
     CDS             5027..5851
                     /locus_tag="VF720_08495"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX7879434.1"
                     /translation="MSESAPVTPVHRAVAELRRRFPEAPDVAVVLGSGLGAFADRLDG
                     AMPMDYGEIPDFPRPTVVGHKGRLVFGRCGGRRVVAMQGRMHLYEGIASDSVTFGVRT
                     LAALGVPTLVLTTASGGIHRHYAAGDIMLIEDQVNLQMRRVGGLSAPAAPAWRGPGPI
                     YSPGLLRKALDVAHATGEFRIHRGVFGGMLGPNYETPAEVRMLRTLGADAVSMSTVLE
                     AVAARDAGMQVLGISAIVNPAAGISPTPLNHEEVLEMAKEMEGRLLAYLAALMPAL"
     gene            complement(6265..7083)
                     /locus_tag="VF720_08500"
     CDS             complement(6265..7083)
                     /locus_tag="VF720_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879435.1"
                     /translation="MRHLDFGRLFGAAAVLTAATFVAAPMASATPNPATASLHLRIFN
                     DDPVSTLTPVNSYPASISITDQKFPGAGGFANRHAWRFSNDGTNYLEFQNADHFRFCA
                     DLTISGTGEAEAGLQIGPWWSPLVDGTFNVRTTDGEIACFGGRMPFYSFTGSHALTYT
                     KGTTIHLEVIYDPNSLSMADPATIEYKVTYNANNYSSGPIAFDQGNPSEDPPHGQWGM
                     LQPSTVGGHVQYFIGGSGPEGGINATWSNICYENLDAVPVEPTTWSNIKSLLQQ"
     gene            complement(7226..8818)
                     /locus_tag="VF720_08505"
     CDS             complement(7226..8818)
                     /locus_tag="VF720_08505"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7879436.1"
                     /translation="MKRILLAGLLVLSLGWPRGAAALTTPALYDTLQHTAFLYFWVEA
                     NPANGLVKDRSTPGSPCSIAATGFGLSAICIGIDHGWVSRTDGRQRVLNTLNTFWTGP
                     QGTAANGTIGYKGFFYHFLDMSIGLRTWDSELSSIDTALLFAGILDAKQYFNTSDPGD
                     IQVRALADSIYYRADWEFFRASGIGLRMGWKPGTGFNGFGNWIGYNEAMIMYLLALGS
                     PTHPPNPVNPTFMWNSWTSGYDWLTEYGYTYINFPPLFGHQYSHCWIDFRYLQDDYTR
                     FRGITYFENSRRATLAQRAYCIANPGGFVGYSGTLWGLTAGDGPNGYSARGAPPAQND
                     NGTIAPTAAVSSLPFTPDEVIPTMHNFFDNYPALWSAYGFRDGFNLTSNPDWYGPDVL
                     GIDQGPIIMMIENHRNQRPWLRFMQNVDIQRGLTRAHFSPVAGVPDAGSPPVALQLAQ
                     NRPNPFSGATLIRYELSEAAPVSLSLFDVTGRVVRNLVDGVQPAGSHELLLDGTDLPA
                     GIYWYRLAVDGRVESRKALRIR"
     gene            complement(8856..>9603)
                     /locus_tag="VF720_08510"
     CDS             complement(8856..>9603)
                     /locus_tag="VF720_08510"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX7879437.1"
                     /translation="VAGAFTFQNVGTFMLGITALNSGDIGVRTVDQPLGTGEQYSVTD
                     LGLSLGYGRQITSRFSAGTQLTYANETIWHSSLHLLTFNIGTVYKLTDDNLQLGACVN
                     NIGTRTSLDGRDLAITFDQDPDAHGDNGTLPATQFTDDFPVPILFRVGLAYPKTLGPR
                     SRILFALDAFHPSDNSESVSAGAEWLFRDTFAVRAGYQQLFQTDSDVGVTLGLGIRGG
                     LGYSRFHVDYAWADHDYLEETHRFTLAVDF"
BASE COUNT         1566 a         2965 c         3415 g         1657 t
ORIGIN      
        1 cactacgagg gaggccgcga ggcggccctc gccctcgaaa cgaccttcgt cccgatcgat
       61 cacgccatcg tggggatcat cgaccgggtg accgtggacg agggcgccac gcgatgatgc
      121 tggcccgtgt gatcggcacc gtggtgtcga ccgtccagca cgcgtccctc aacgggcgcc
      181 gccttctcct tgcccggcgg ctcggcctcg atggcaagcc caccggcagc acgctcatcg
      241 ccgtcgacac ggtccaggcc acgcccggcc agacggtgct cattctcgac gaggggaatg
      301 gggcgcggca ggtgctgggg gcgccggagg cgccgatccg gacggtgatc gttggagtgg
      361 tggaccgcgc cggttagtcg aggtcgcagc cgctatcgca ggttcttgat cctggaccag
      421 gtcgtcggct caagagatcc cggagccggt acgcccgacg tggccaccgt cacgtagatg
      481 tcgtcgatcc ccacccactc gtcgttcccg acggcgttgg ctgtcatgat ccggacctgg
      541 agctgcgggc ggttgccgac gacgcccgga aggacgatcg acaccatcgt ctgcagagta
      601 ttgaggccgg gcccgtgcgt cacgtcgaag aagtccccgc cgacggcatt cgtccacgaa
      661 ccgctcgcct gcgtgcggta ctgcacctgg atcggctgct gcgtgttgtc gagggagccg
      721 tcgaggtccc ggaggcgaca ctggaacacc agcggggccg gaatcccggt cgtgttgagg
      781 tggatgacca ggtaggggtg atcggccgtg gccgaggcct ggagcgccac gacgggatcg
      841 gtcagctggc actcggccac accgccgatg atgatcccgg acggatcccc ctggttcggg
      901 atcaggtcgg gcgcgctcgt cgaatcggcc gtgatggtgc gaggcagacg cccgggagtg
      961 gactcgatcg gattctcgcc gaggtagccc acgatgcccg ggacgttgtt ccagttgtcg
     1021 ctggtcgaaa gaagagcggt gttcgaccag ttctggaaga aggggatgga ctggtaagca
     1081 gtattggacg cggccggcat cgcgtgagcg catgcggcca gcagtgtggc gatcaggatg
     1141 cgatgggtta ttttgcgcgg cgccattttg gttgacaaaa cgcccgggtc gaggtccctc
     1201 ggcccgggcg tgtacggtct ttgatgatgt cgaaggacta gttcgagccc ttcttgatcg
     1261 cgctccagct cgacgtctcg gtcgggaccg ggaggttcgt gccggtgatc gagatgtcgt
     1321 cgatgccaat ccactcgtcg ttgccgaccg agttggtggt caggacgcgg atctgcactt
     1381 cgggctggtt gttggcgccg gccgggagca ccgccgaaac cggggtgacc agggtggcga
     1441 gcgacgggcc ggtggtggcg tcggcgacgg aggtcacgtt cgtccagttg ccggcgttgc
     1501 cgacgcggta ctgcacggcc accggggtgt tggcgttgtc ggtcgagccg tcgatgtcgc
     1561 gcaggttgca attgaaggtg atcgactgca ggccggtcgt cacgacgtgg aagcggaggt
     1621 gcgggaagtc ggcagtgccg gagccctgca gggccacgac cggattggtg atgtggaatt
     1681 cgcccacgcc accggccgtt gaggtgttcg ggttggtgcc gttggcgatc acgtcctgaa
     1741 cgccggagct gtcgcccacc agagaggttg ggttgacgcc cgtgacgctg gtaaccggat
     1801 tgtcgcccag gaagccgagg atgcccggga cacccgacca gtcatcgttg gccgtgatga
     1861 gcccgatgtt cgtccagtcc tgcgagaagg ggaggccctg cggagtgccg gtggcaaaag
     1921 cattgccggc ggcaccgatg agagcgacag caacgagcgt cagtaccttt gccttcattt
     1981 gggtcttacc tccctggtgc gcgccgggac ttatgtccgg acttcggaca acacaagtcc
     2041 ttgctgcata tacggttgca atgatcaatg aatgttgagg tagaggaggt gggcggcgag
     2101 acggcggccg ttccggagcc ctgatcctgt tagggaaata ccttagcacg ggccgtatgt
     2161 cgcgttccag acataatttg gtcgattccg tgacgagttt tctgcggaaa tcggccgccc
     2221 ccgggcggcc cccgatggcg acagccggac acggttgctt aagcctgccg gcttcggcaa
     2281 gatgccggcc ggttcgacac ttcgagaggc gcgggggcat tcatgcacga cgacatcgac
     2341 cggatcctgt acaccgacga cgacatccag aaccgcatcc gcgccctggc gcgcgacatc
     2401 aatgaggcct atccgaccgg cgacctggtg ctgctcggcg tcctgtcggg gagcctcctt
     2461 ttcctggccg acctggcccg ggccctcgat cggcacgtca tcgtcgactt catcgcggtg
     2521 tcgtcgtacg aggggggcac ccagtcgacg ggcgtcgtcc ggctcctcaa ggacctgtcg
     2581 gtcaacatcg aggggaagga tgtcctgctg gtcgaggaca tcatcgacac cggcctcacg
     2641 atcaactacc tgatcgagaa cctcaccacg cgtcacccgc gttcgctggc ggtgtgcgcg
     2701 ctgctcgaca aggccgaggc gcgcaaggcg ccggtgccga tccgcttcac cggcttcacg
     2761 gtgcccaacg agttcctcgt gggttacggg ctcgattacc agcagcgtta tcgcaacctg
     2821 ccgtacgtgg ggatcctcaa gccgcgcgtc ttccagcccg ccggttcatg acgacgcccc
     2881 acggggcgcc ccgaattccc ggcggcgggc tcctcttccg gaatggccgc tggatcacca
     2941 tggatgccgc gcgccgggtg gtggacgggg actgctacgt cgtcgatggg cgcattgagc
     3001 acctcggcgg gcgcttcgag gctcctgagg ccacgccggt caccgatttg tccggccgct
     3061 gggtgacgcc cggcttcatc cagggtcacg tccatcttgg ccagacgctc ttccgcggcc
     3121 tcggcgagaa ccgtcggctg ctcgactggc tcaaggacgt gatctggccg ctcgaggccg
     3181 cgcacgacga cgattcggcc tactggtcgg ccgtggttgg cggcatcgaa gcgatgcggg
     3241 ccggtacgac cgccatgctc gacatcggcc tggtccatgg cgcggccggc ctgttcaagg
     3301 ggttcctcga cctcggcatc cgcggctgga ccggcaagct catgatggat cgcggcgatg
     3361 gcgtgccgaa ggggctgctc gccgatccga atcacatcct ggccgagagc catgagctgg
     3421 cccgcaagtg ggacggcgtg gacaacggtc gtctccgtta cgcggtctgc cctcgcttcg
     3481 tcttctcgtg ctcgccggcg ctctgggagc gggcggtggc cgaagcgaag gggggggact
     3541 ggccgatcca cacgcacgcc tttgaaacgc gtgaggagca ggaagcggtg cgggccatcg
     3601 agcatcggag cgagctcgac acgctggccg atgtcggcgc gctcggcgcc tcgctcaagg
     3661 tggcgcacgg catctgggtg gatgatccga cggccgcgcg gcttggcggc gcgtcggcct
     3721 ccgtcatcca ctgcccggcc agcaacctga agctcggttc gggcgtggcc gacctgacgc
     3781 ggctcaaggc acacggagtg aaagtgggga tcgggtgtga cggatcgccc tgcaacaact
     3841 tcctcgatcc cttgggggag atgcggcttg ccgcgctcct ccagaagtgg aagcacgggc
     3901 cggaggcgta tggcgcggcc gatgcgctgg cactggccac gtgtgacggt gcccgggcgc
     3961 tcgacgccga ggaccggatc ggctcgatcg aagtggggaa gcgggccgac ttcgtggtct
     4021 tcgacctgga acgggtgcac tcgattgccg cggacgccgt cgacccgtac acgcggatcg
     4081 tcttcggcgc cgaccggagc aacatcgagt cggtgtacgt tgaagggcgc gccgtggtgg
     4141 agcggggacg cgtaccgggc ttcgacgagg cggaggtggt gaccaagggg aaggagtcca
     4201 tcgcggcggt gctggcccgg gcggggcggt tcggatcggg cacgggccct gttaagatgg
     4261 cgccatgaga gccacccacc gccgggccga tgctgcatgc gggcccgccc tcacctgccg
     4321 gaccgccgtc acatgaatcc tcccgcctgc tggatcatcg ggatcgccgg tggctcggcc
     4381 tccggcaaga cccgcgtggc tcacgccatc gtcgaggagc tggggccgga cgaggtgcag
     4441 gtcatcgacc aggatgccta ttaccgcgac ctcgcgcatc tcccgatgga ggaccggaag
     4501 cggcagaact tcgatcaccc ctcggcgttc gacaccgagc tcatgctcga acacctgacc
     4561 gtgctgcgcc aggggggctg ggtggacaag ccggtgtacg actacgccgc ccacacccga
     4621 tccagccgaa cggaacgggt gggagggcac gccgtgctgg tgctcgaagg gatcctggtg
     4681 ctggatgatc cccgcctgcg cgcgctcatg agcgtgaagg tgttcatcga caccgacccc
     4741 gacgtgcgct tgatccgccg cctccgccgc gacgtcaccg agcgcggccg ctcggtggag
     4801 tcggtgctcc agcagtacga ggcctcggtc cgacccatgc accagcaatt cgtcgagcct
     4861 tccaagaggc atgccgacat catcatcccc gagggggtgg agaaccacgt gggcgtggat
     4921 atcttgagaa cgaaggtgca cgcactggcc aacgcgtcgg cgcgaaacgc acgggccgac
     4981 ctgacgccat cgcggtgagc gagtccattc ccatgggggt aagcccgtga gcgagtcggc
     5041 tcccgtcacc ccggtccatc gggccgtcgc cgaactgcgc cgccggttcc ccgaagcgcc
     5101 ggacgtggcc gtggtgctcg gctccgggct cggcgccttc gccgatcggc tcgatggggc
     5161 catgccgatg gactacggcg agatccccga ctttcctcgc ccgactgtgg tgggccacaa
     5221 gggtcggctg gtcttcggcc ggtgcggtgg ccggcgcgtc gtggccatgc aggggcggat
     5281 gcacctctac gagggaatcg cgtccgactc ggtgacgttc ggcgtgcgca cgctggcggc
     5341 gctcggggtg ccgacgctgg tgctcacgac cgcttcgggc ggcatccatc gccactacgc
     5401 cgccggcgac atcatgctga tcgaggacca ggtgaatctg cagatgcgcc gcgtcggcgg
     5461 cctgtcggct cccgcggcgc ccgcgtggcg cgggccgggg ccgatctatt cacccgggct
     5521 gctccggaag gccctggacg tggcacacgc caccggcgaa ttccggatcc accggggagt
     5581 gttcggcggc atgctcgggc ccaactacga gacccccgcc gaggtccgga tgctgcgcac
     5641 gctcggcgcc gacgccgtgt cgatgtccac cgtcctcgaa gccgtggccg cccgcgacgc
     5701 ggggatgcag gtgctcggca tctccgccat cgtgaacccc gccgccggca tctcgccgac
     5761 gcccctcaac catgaggagg tcctggagat ggcgaaggag atggaagggc ggctgctggc
     5821 ctatctggcg gcattgatgc cggcgctgta ggggtgggtc ggaggcgact ccggaaccgg
     5881 cgccggaacc ggcgccggtg atggttggcg gcgctgccgg ctcactttca ccggtgaaag
     5941 tgcgtactgt gtgacaaaca ggtgtccggg gtttcgctgc tgaccccgct gtggtcccag
     6001 tgtcgtcccc ctggcatcca ctccggcatc atcctcggca tcgtccgcgc cgtcgaccct
     6061 ggcagcaaca caagcgtcaa ccccggcagc aggcccggca tcagccgaac agcatcccat
     6121 gacccgcaac tttcaccggt gaaagtcgcg gaaagtggca tcccgtcgac cccggcacag
     6181 ccaacaaaaa gaggggggcc gaagcccccc tctcgaattc tgcgtccccc gcgaagcggg
     6241 gggcgtgtct tccctggaac cggtctactg ctggagcagc gacttgatgt tgctccaggt
     6301 ggtcggctcg accgggacgg catcgaggtt ttcgtagcag atgttggacc atgtggcgtt
     6361 gatgccgcct tcgggccccg atccgccgat gaagtactgg acatggccgc cgaccgtgga
     6421 cggctggagc atgccccact ggccatgcgg cggatcctcg gaggggttcc cctggtcgaa
     6481 ggcgatcggg ccgctcgaat agttgttggc gttgtaggtg accttgtact cgatcgtggc
     6541 cggatcggcc atcgaaaggc tgttcgggtc gtagatgact tcgaggtgga tggtggtgcc
     6601 cttggtgtag gtcagcgcat ggctgccggt gaagctgtag aagggcatgc ggccgccgaa
     6661 acaggcgatt tctccgtcgg tggtacggac gttgaaggtg ccgtcgacga ggggcgacca
     6721 ccacgggccg atctgaaggc cggcttcggc ttcaccggtg ccggaaatcg tgaggtcggc
     6781 gcagaaccgg aagtgatcgg cattctggaa ctcgaggtag ttcgtgccgt cgttcgagaa
     6841 gcgccacgcg tggcgattgg cgaacccgcc ggcgccgggg aacttctggt ccgtgatgct
     6901 gatcgaggcc ggataggaat tgacgggagt gagggtcgaa accggatcgt cgttgaaaat
     6961 acggaggtgc agcgacgcgg tggcggggtt gggggtggcc gacgccatgg gcgccgccac
     7021 gaatgtcgcg gcggtcagga ccgctgctgc cccgaaaagt ctcccgaaat caaggtgtct
     7081 cactgggtgc tcctttcgca tgtaactgtt tacatgggat gcgagacaaa ggggggaccc
     7141 tttgccggat gtcgcttggt gcgctgggaa gaatatcacc tccccggcgc actccaagcg
     7201 tgaaaaccgt gaaaacccct gattttcagc ggatcctcag ggcttttcgg gattcgaccc
     7261 ggccatccac ggccaggcgg taccagtaga tgccggccgg aaggtcggtg ccatccagca
     7321 ggagttcatg gctcccggcc ggctggacgc cgtccaccag gttcctgacc acccgacccg
     7381 tgacgtcgaa cagcgaaaga ctgaccggcg ccgcttcgga gagctcgtag cggatgaggg
     7441 tggcccccga gaacgggttg ggacggttct gggccaactg gagggcgact ggggggctcc
     7501 cggcgtccgg cactccggcc acgggggaga agtgggcccg ggtgaggccc cgctggatat
     7561 ccacgttctg catgaatcga agccagggac gctggttcct gtggttctcg atcatcatga
     7621 tgatcggccc ctggtcgatc cccagcacgt ccggaccgta ccagtccggg ttcgacgtca
     7681 ggttgaagcc atcccggaag ccgtaggcgg accagagggc gggataatta tcgaagaagt
     7741 tgtgcatggt cggaatcact tcgtccggcg tgaacggcag tgagctcacg gccgcggtcg
     7801 gggcgatggt gccgttgtcg ttctgcgcgg ggggcgcgcc gcgggccgag tagccgttcg
     7861 gtccgtcgcc ggcggtgagc ccccacaacg tgccgctgta gccgacgaag ccgcccggat
     7921 tggcgatgca gtacgcgcgc tgggccagcg tggcccgacg cgagttctcg aagtaggtga
     7981 tcccccggaa acgggtgtaa tcgtcctgca ggtaacggaa gtcgatccag cagtgcgaat
     8041 actggtggcc gaaaaggggc gggaagttga tgtaggtgta gccgtactcg gtgagccagt
     8101 cgtagccgct ggtccagctg ttccacatga aggtgggatt taccggattg ggcggatgcg
     8161 tgggggaacc gagggccagg agatacatga tcatcgcctc gttgtaaccg atccagttgc
     8221 cgaagccgtt gaaacccgtt cccggcttcc atcccatccg gagcccgatg ccggacgccc
     8281 gaaagaactc ccagtcggcc cggtagtaga tcgagtccgc cagcgctcgc acctggatgt
     8341 cgcccgggtc ggacgtgttg aagtactgct tcgcgtcgag gatgccggcg aagagaagcg
     8401 ccgtgtcgat gctcgaaagc tcgctgtccc aggtgcgcag gccgatcgac atgtcgagga
     8461 agtggtagaa gaaacccttg tagccgatgg tgccgttcgc cgcggtaccc tgcgggcccg
     8521 tccagaaggt gttcagcgtg ttcaggacgc gctggcggcc gtcggtgcgc gatacccagc
     8581 catggtcgat gccgatgcag atggcggaga ggccgaagcc ggtggccgcg atgctgcagg
     8641 gcgagccggg ggtgctccgg tccttcacca ggccgttggc cggattggcc tccacccaga
     8701 agtagaggaa agcggtgtgc tgaagcgtgt cgtagagggc gggggtggtg agcgccgcgg
     8761 cgccccttgg ccagccgagc gacaggacca ggagccccgc cagcagtatg cgtttcatgg
     8821 tgtcctttcc ggggtctgcc acgagcttac gccgatcaga agtcgacggc cagcgtgaaa
     8881 cggtgcgttt cctcgagata gtcatggtcg gcccaggcgt aatccacgtg gaaccggctg
     8941 tagccgagtc cgccgcgaat gccgaggccc agggtcacgc cgacatcgct gtcggtctgg
     9001 aacagttgct ggtagccggc ccgcaccgcg aaggtgtcgc ggaagagcca ttcggccccg
     9061 gcgctcacgc tttccgagtt gtcgctcgga tgaaaggcat ccagcgcgaa gagaatcctg
     9121 ctgcgcgggc cgagcgtctt cgggtaggcg agtcccaccc ggaagaggat cggcaccggg
     9181 aagtcgtcgg tgaactgcgt ggccggcagc gtgccgttgt cgccgtgggc gtcgggatcc
     9241 tggtcgaagg tgatggccag gtcgcggccg tccagcgagg tgcgcgtgcc gatgttattg
     9301 acgcaggccc cgagttgcag gttgtcgtcg gtgagcttgt acaccgtgcc gatgttgaag
     9361 gtcaggagat gcagcgacga gtgccagatc gtctcgttgg cgtaggtgag ctgtgtgccg
     9421 gccgagaagc ggctggtgat ctgccggccg tagccgagtg acagcccgag atcggtcacc
     9481 gagtattgct cgcccgtgcc gagcggctgg tccaccgtgc gcacgccgat atcgcccgaa
     9541 ttgagtgcgg tgatgccgag catgaaggtg ccgacgttct ggaaggtgaa ggcgccggcc
     9601 acg
//
LOCUS       DASVOD010000369         2335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_443395, whole genome shotgun sequence.
ACCESSION   DASVOD010000369 DASVOD010000000
VERSION     DASVOD010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443395"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /gene="pilQ"
                     /locus_tag="VF720_08515"
     CDS             <1..1770
                     /gene="pilQ"
                     /locus_tag="VF720_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX7879438.1"
                     /translation="DIPGANHSLPRTAEMETGLMTGIQTDIWHDEEGTPVTRVTVHLK
                     SPATSWVESSQNGFQIMLTPAALELPAGQAAAVSAPTSSEMPASIDAMAEQAAAEMST
                     ESPSTDGTTSSATSADGSTTSDFSPTWGEVTVAGSAPAATATATSDPSMTEDASAAPS
                     QGSAVQQMLAERPLEFTPRSLQDGTRYAESGSRVSLDVQGADIRTVMRSLAEYSGTNI
                     VVGKEVTGAVTVNLQEVPWIDALNTICRTQSLGWVDEEGIIRIETLENLRKEDVARSS
                     ADRQLEDLQPLTTQIVRAVYATATELKPAIEKTLTNRGHIEVDPRTNSLVITDVSRRV
                     SAAAEMVKHLDSRTPQIEITAKLVDVDARYTRDLGISWSVGQLHSTDNAFSAEGDVNA
                     NGVINPAANVRFGLVRSWGTINAMLSALEQDSKADIISNPRITTVNNREARILVGRKI
                     PLIVLDEAGNAITQLTTIGITLLVTPHINDDERITLDLHPEVSDLASQATVQGGIIIN
                     TSEADTRVIVDDGETAVIGGLIRSNIGKVTRGVPVLKSIPIIGSLFRSTSDVKEKREL
                     LIFITPTIVTNWNQTSDASSYKE"
BASE COUNT          424 a          862 c          705 g          344 t
ORIGIN      
        1 gacatcccgg gtgccaatca ctcgctcccc cgtaccgccg agatggaaac cggcctcatg
       61 accggcatcc agaccgacat ctggcacgac gaagagggga cgccggtcac ccgagtgacc
      121 gttcacctca agtcgccggc caccagctgg gtggaatcga gccagaacgg cttccagatc
      181 atgctgacgc ccgccgcgct ggagctcccc gcggggcagg cggcggcggt ttcggccccg
      241 acctcgagcg agatgccggc gtccatcgac gcgatggccg agcaggccgc cgccgagatg
      301 tccacggaga gcccgtcgac cgacggcacg acctcgtccg cgacgtcggc cgatggaagc
      361 accacctccg atttctcccc gacctggggc gaagtgaccg tggccggctc ggctcccgcc
      421 gccaccgcca ccgccaccag cgacccctcg atgaccgaag acgcgtccgc tgcaccgagc
      481 cagggttcgg cggtccagca gatgctggcc gagcgtccgc tcgagttcac gccccgctcg
      541 ctccaggacg gcacgcgtta cgccgagtcc ggctcgcgcg tgtcgctgga cgtgcagggc
      601 gccgacatcc gcaccgtgat gcgctcgctg gccgagtatt cgggcacgaa catcgtcgtc
      661 ggcaaggaag tcaccggcgc cgtcaccgtc aacctccagg aagtgccgtg gatcgacgcc
      721 ctcaacacca tctgccgcac gcagtcgctc ggctgggtgg acgaagaggg gatcatccgc
      781 atcgagacgc tggaaaacct ccgcaaggag gacgtggccc gctcatcagc cgaccgccag
      841 ctggaggacc tccagccgct gacgacgcag atcgtgcgcg ccgtgtacgc caccgccacc
      901 gagctcaagc ccgccatcga gaagacgctg accaaccgcg gccacatcga ggttgacccg
      961 cgcacgaact cgctggtcat caccgacgtg tcgcgccgcg tgtcggccgc cgccgagatg
     1021 gtcaagcacc tcgattcgcg caccccgcag atcgagatca ccgccaagct cgtcgacgtc
     1081 gacgcccgct acacccgtga cctcggcatc tcgtggagcg tgggccagct gcactccacc
     1141 gacaacgcct tctcggccga gggcgacgtg aacgccaacg gcgtgatcaa cccggcggcc
     1201 aacgtccgct tcggcctggt ccgctcgtgg ggcaccatca acgccatgct gtcggccctc
     1261 gagcaggaca gcaaggccga catcatctcg aacccgcgca tcaccacggt gaacaaccgc
     1321 gaggcgcgga tcctggtcgg ccgcaagatt cccctcatcg tgctggacga ggcgggcaac
     1381 gccatcaccc agctcacgac gatcggcatc acgctgctgg tcacgccgca catcaacgac
     1441 gacgagcgga tcacgctcga cctgcacccc gaggtttcgg acctcgcgtc gcaggccacc
     1501 gtccagggcg gcatcatcat caacacctcg gaagccgaca cccgcgtgat cgtggacgac
     1561 ggcgagacgg cggtcatcgg cggcctgatc cgcagcaaca tcggcaaggt cacccgtgga
     1621 gtgcccgtcc tgaagagcat cccgatcatc gggtcgctgt tccgttcgac gagcgacgtg
     1681 aaggagaagc gcgagctcct catcttcatc accccgacga tcgtgaccaa ctggaaccag
     1741 acgagcgacg cctccagcta caaggagtaa tcgttcggcc cccggggaag gggccacatg
     1801 aagcctcagg cgagttcccc ccagctcgcc ggaagacgcc gccggtcgac gcaagtcggc
     1861 cggcggctcg cttttgttta cggtctgtcg ttcgcgaatg acagcggtgt ggagaaacgc
     1921 cacgattctc tgagcaactt ctgcagtaca gacgggacgc cggctgcgta cccgccgatt
     1981 tcctcataac tccttgtctt tccgcacaaa agtagtggaa tcccccatca gcccctcatt
     2041 agtatggcgc cctgggtttc caatcagggc ttgccccagt ccctccggcg tcccccgccg
     2101 gtggcggcgc tccctgtccg tcacccctga ataccgttcg gcgtgtgccc ctgccggacg
     2161 gaccacacct gggaggactg tccatgcacc gcacgctacg catttccctg tccgccgtac
     2221 tgggcctcat ggcgttcgcc ggcatcgccg gcgccaacga catggccaag ctcgacccgc
     2281 gcgcccgtgt ggccctgcgc gtggccgagt cggttaactc gccgggcggc aagat
//
LOCUS       DASVOD010000370         6386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_286787, whole genome shotgun sequence.
ACCESSION   DASVOD010000370 DASVOD010000000
VERSION     DASVOD010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286787"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2153)
                     /locus_tag="VF720_08520"
     CDS             complement(<1..2153)
                     /locus_tag="VF720_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX7879439.1"
                     /translation="MTRFRRLSPTLLIGAILMVLVVVVACENELLGPADDTSLPACQV
                     RDADLDFGETFVGQVSEDRSFTVRNIGGGVLAGQVVLPPGAFEIISGGGSFSLAPGQL
                     RRVLVRFRPTTVGAAEAELRIGTGCRFVALSGMGMTGDLCRVSRDTLDFGSILVGEGA
                     VERSFTLFNDGQTTLSGMLSAPCGSYTIVSGGGAYQLVPGASRVVTVRFAPTGAGTDN
                     CLVETGLAACADVVLIGEGLNQFCEVTPGSLSFGEVCVGERPERMITLRNPGTAPISG
                     TVGLAIFPFQTTSGAGAYTLQPGQSREITVIYRPDQAGSHTGSLPLGGLCGNVPLDGS
                     ASRPFVCQLSTNELRFDNVPTNSSETQSFTIVNSGCGNGLVAVPALCGGVFDLGPERN
                     IAIGPGQSVTLPVIFRPASDAPVECLLDLGTACGPLRLVGSGMTVPVCRLTPEVVDFG
                     PVTVGQNTTRTFTIENAGDGILSGVVPGGCDNFTVTAGSGSFSLGPRQTRSVTVRFQP
                     TGPGIRSCVLSLGTGQCTGVTLNGLGDAAPACEITPSTLLFGGVVVNTNSEKSFVVRN
                     AGGGMLNGTISENCNGFEVISGGGAYSLAGGESRTVTVRFRPTVQGMQTCGIGLGSLP
                     CNNVAATGTGTAAGLCRLQPVDLDFGTVFVGETADRTFTLSNLGGAPISGNIAESCGE
                     FSIVSGGGAFSIGQNQSVTVTVRFAPTGTGTRTCS"
     gene            2311..3618
                     /locus_tag="VF720_08525"
     CDS             2311..3618
                     /locus_tag="VF720_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879440.1"
                     /translation="MRSIVAVSCLLLGLFPAPAAHAQSLNLRDLLPEFLRRGVTLAPP
                     AVGTDHSAHFNSAANQFDAVDQINSEIAYQLSSFPLSSSAGGFAYTYDAENGVFSRPT
                     RSFGPVYTERAFNVGRGKINVGVNYSRFTYDAIDGIDMAGTGLPFVFQHIDSNNDGTN
                     TDFFFEGDLVRADVYVSVESSITALVTTYGVSDNFDVGLAVPAVDVNMDVRVDAYVER
                     LATEDNASDTHAFPNGTPHSTTQTGGSANGIGDVQVRAKYRFTEGKALAALLGEMRLP
                     TGDEDNLLGVGAFGARIAFVGTYNSDVVAPHINVGYQSNAEGLPDEITYRFGADWVMD
                     PKITLSGELLGLAQSGVTDVATENVTYQANTNPTGPPHIVEGTFSQTTYTPDQDRSTL
                     TAAIGVKINLARTLLLSLDGTFPLTDKGLRDDFTPLLGLDYSF"
     gene            3784..4290
                     /locus_tag="VF720_08530"
     CDS             3784..4290
                     /locus_tag="VF720_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HEX7879441.1"
                     /translation="MANPIYIWYKGVTQGPIEGFGSWAGEDDQAGKEGSSLVLSLTHE
                     VTSPRDSATGLASGRRIHQPLTITKRIDKASPKLYMALTKNEQLSEVTVKWFRVVQAG
                     GGGQEHYFTTQLFGAVVTNIKQWFPIISDPTKREYSHLEDVSFSYNKIIWTWVDGGIT
                     AEDDWRAG"
     gene            4287..5087
                     /locus_tag="VF720_08535"
     CDS             4287..5087
                     /locus_tag="VF720_08535"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX7879442.1"
                     /translation="MIPQEDDKAWTVLGVELGTPDGPLRGQVQIPTGPLTLSEIVPSA
                     LELTNILASRANARSERRGLPVSCRAGCGACCRQMVPLSIPEVFYLMERIAEMEPAAR
                     DVAVNRFMAIDRRLDMAGMVEPLLDLKVAADPQRALNQTYFEMKMACPFLVDESCSIH
                     SVRPVACREYSVTNNPKFCANPYAGGIDKVPMPLPLSAALAWLASERLGVPPMLIPMT
                     LVPRWASENAELAEQRWPGVELFSQFMKIAGSPPPEWAMNPDAPPAGN"
     gene            5237..5845
                     /locus_tag="VF720_08540"
     CDS             5237..5845
                     /locus_tag="VF720_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879443.1"
                     /translation="MLGRISFALLTICACTLTSTARADIDVFINFDRDTNGIALESPA
                     VINEVYLSWGVTFGSNPGAQCGSGEVYATSACLSEPAPTSAPNIVSFCAGCSDISEDT
                     GYVIATFLMPADSVCITAIAAEPNGRSYLRAYDAEHNLVVEDISNPELASDVLCVTGP
                     AIHTVEFTGLPGEFAWFDDMQVSFAEVPVEPVTWSGIKARER"
     gene            6146..>6386
                     /locus_tag="VF720_08545"
     CDS             6146..>6386
                     /locus_tag="VF720_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879444.1"
                     /translation="MIRRLLPLAACALALGLTTAAHAGIDVVINFDTDTNGNPIPHGT
                     IVNNLYAPWGVNFDRTVPGSCGSESGAFVFTSNECL"
BASE COUNT         1167 a         2253 c         1945 g         1021 t
ORIGIN      
        1 atcgagcagg tgcgcgttcc cgttccggtc ggcgcgaagc gcacggtcac cgtgacgctc
       61 tggttctgtc cgatcgagaa cgccccgccg cccgacacga tgctgaactc gccgcacgac
      121 tcggcgatgt tgcccgaaat gggagcgcca cccaggttgg aaagcgtgaa ggtgcggtcg
      181 gcggtctcgc cgacgaacac cgtgccgaag tcgagatcga ccggctggag gcgacagagc
      241 ccggccgccg tcccggtgcc ggtggccgcc acgttgttgc atggcaacga cccgaggccg
      301 atgccgcagg tctgcatccc ctgcaccgtc ggccggaagc gcaccgtaac cgtgcggctc
      361 tcccccccgg ccaggctgta ggcgccaccg ccggaaatca cctcgaagcc gttgcagttt
      421 tcggagatgg ttccgttcag cattcccccg ccggcgttgc gcaccacgaa cgacttttcg
      481 gaattggtat tcaccaccac gccgccgaag aggagggtgc tcggcgtgat ctcacaggcg
      541 ggcgccgcgt cgcccaggcc gttcagcgtg acgcccgtgc actggccggt gccgagcgac
      601 agcacgcacg agcggattcc cggcccggtc ggctggaagc ggacggtgac cgaccgcgtc
      661 tggcgcgggc cgagcgagaa actgccgctg ccggcggtca ccgtgaaatt gtcgcagcca
      721 ccgggcacca cgccggagag gatcccatcc cctgcgtttt cgatggtgaa ggtccgggtc
      781 gtgttctgcc ccaccgtgac cgggccgaag tcgacgactt ccggcgtcaa ccggcagacc
      841 ggcacggtca tgccgctgcc gaccaggcgg agaggaccgc aggcggtgcc gagatcgaga
      901 aggcactcga ccggagcgtc gctcgcggga cgaaagatga ccggaagtgt gaccgactgg
      961 ccggggccga tggcaatgtt gcgttccgga cccaggtcga acacgcctcc gcaaagtgcc
     1021 gggacggcca ccaggccgtt cccgcagccg ctgttgacga tggtgaagct ctgcgtttcc
     1081 gagctgttgg tcggcacgtt gtcgaaccgc agctcgttcg tgctcagctg gcagacgaac
     1141 ggccgggagg cacttccgtc gagcggcacg ttcccgcaca agccgcccag cggcagcgag
     1201 ccggtgtgtg aaccagcctg gtccggccga tagatgacgg tgatttcgcg gctctggccg
     1261 ggctgcagcg tgtacgcgcc ggcgccgctc gtcgtctgga atgggaagat ggccaggccg
     1321 accgttccgg aaatcggcgc cgtgcccggg ttgcggagcg tgatcatccg ctcggggcgc
     1381 tcgcccacgc agacctcccc gaacgaaagg ctccccggcg tcacctcgca gaactggttc
     1441 aacccctcgc cgatcaggac gacatcggca caggccgcca ggccggtttc caccaggcag
     1501 ttgtccgtgc cggcgccggt gggcgcgaag cgcacggtca cgacgcggga cgctccgggt
     1561 acgagctggt acgcgccgcc ccccgacacg atcgtgtagc tgccgcacgg cgccgagagc
     1621 attcccgaga gggtcgtctg gccgtcgttg aacagcgtga agcttcgctc caccgccccc
     1681 tcgcccacca ggatcgaccc gaagtcgagc gtatcccgac tgacacggca gaggtcgccg
     1741 gtcatcccca tgccgctcaa ggccacgaac cgacagccgg tgccgatccg aagctcggcc
     1801 tcggccgcgc cgacggtggt gggccggaac cgcaccagca cccggcggag ctgccccggc
     1861 gccagggaaa acgagccgcc gccggagatg atctcgaaag cgccgggcgg caggacgacc
     1921 tgcccggcca ggaccccgcc gccgatgttg cggacggtga agcttctgtc ctctgatacc
     1981 tggccgacga acgtctcccc gaaatcaaga tcggcatcgc ggacctggca ggcgggcagc
     2041 gaggtatcgt cggccggccc gagaagctcg ttctcgcagg ccacgaccac cacgagcacc
     2101 atgaggatcg ccccgataag gagcgtgggg gagagacgac ggaaccgcgt cacgaggcgg
     2161 agtatcgtgc gcctccctga acctgtcaac gaaatgaggt cacgcgtcac tggcaatccg
     2221 tcgttgacgc ccccccgcag gcggtgccaa gatgccgcgc gatcccaccg gaattcgaat
     2281 catggcgtcc tggtcccgaa cggaggcctc atgcggtcga ttgtcgccgt cagctgtctg
     2341 cttctcggtc ttttccccgc gcctgccgcc cacgcccaga gcctcaacct gcgcgacttg
     2401 cttcccgagt tcctccggcg gggcgtcacg ctggcgcctc ccgcggtcgg caccgatcat
     2461 tccgcacact tcaactcggc cgccaaccag ttcgacgcgg tggaccagat caactcggaa
     2521 atcgcctacc agctctcatc gttcccgctt tcgagttcgg ccggcgggtt cgcctacacc
     2581 tacgacgcgg agaacggtgt cttcagccgc cccacgcgca gtttcggccc ggtgtacacc
     2641 gagcgcgcgt tcaacgtggg gcgcggcaag atcaacgtgg gcgtcaacta cagccgcttc
     2701 acgtacgacg ccatcgacgg aatcgacatg gccggtaccg gcctcccctt cgtcttccag
     2761 cacatcgact cgaacaacga cggtaccaac accgatttct ttttcgaggg ggatctcgtg
     2821 cgggccgacg tgtacgtaag cgtggagagc agcatcaccg cgctggtcac gacttatggc
     2881 gtatccgaca acttcgacgt gggcctggcc gtgccggcgg tggacgtgaa catggacgtc
     2941 cgggtcgacg cctacgtgga gcgcctcgcc accgaggaca acgcctccga cacgcacgcc
     3001 ttcccgaacg gcacgcccca cagcaccacc cagaccggcg gcagcgccaa cggcatcggc
     3061 gacgtccagg tgagggccaa gtatcgtttc acggaaggaa aggccctggc ggccctcctc
     3121 ggcgagatgc gcctccccac cggcgacgag gacaacctgc tcggcgtcgg tgccttcggc
     3181 gcccgcatcg ccttcgtcgg cacctacaac agcgacgtgg tggccccgca catcaacgtg
     3241 gggtaccagt ccaacgccga ggggctcccg gacgagatca cctaccgctt cggtgccgac
     3301 tgggtgatgg atccgaagat caccctctcg ggcgaattgc tcggcctcgc gcaatcgggc
     3361 gtgaccgacg tggccaccga gaacgtgacg taccaggcca acaccaaccc gaccggccct
     3421 ccgcacatcg tggaggggac gttctctcag accacctaca cccccgacca ggaccgcagc
     3481 accttgacgg cggccatcgg ggtcaagatc aacctggccc gcaccctcct tctctcactg
     3541 gacggcacgt tcccgctcac cgacaagggg ctgcgggacg acttcacgcc cctcctcggg
     3601 ctggactaca gcttctagac ggcctttccg gccggtcgaa cctgaattga cagtattccg
     3661 gaatgggcct aaatttcggg ccggccgggg tgatgtcccg aatggcaatc tccccggcaa
     3721 catcgatcct gaagggttca gggcaatcga atccgggcac cagccgcacc acgaggggtc
     3781 cgcatggcta atccaattta tatctggtac aagggcgtca ctcagggacc gatcgaaggg
     3841 ttcggctcct gggccggtga agacgatcag gccggcaagg aaggctccag cctcgtcctc
     3901 tcgctgaccc acgaagtcac cagcccgcgc gattccgcca ccggtctggc cagcggccgc
     3961 cgcatccacc agccgctcac gatcaccaag cgcatcgaca aggcctcgcc caagctctac
     4021 atggcgctca ccaagaacga gcagctctcc gaggtcaccg tgaagtggtt ccgcgtcgtc
     4081 caggccggcg gcggcggaca ggagcactac ttcaccacgc agctcttcgg agccgtggtc
     4141 accaacatca agcagtggtt ccccatcatt tccgatccga ccaagcgcga atactcccac
     4201 ctcgaagatg tctcgttcag ctacaacaag atcatctgga cgtgggtgga tggcggcatc
     4261 accgccgagg acgactggcg cgccggttga tccctcaaga agacgacaag gcctggaccg
     4321 tcctgggtgt cgaactcggc accccggacg gcccgctccg cggccaggtc cagatcccca
     4381 ccggtcccct caccctctcc gaaatcgtcc cctccgcact cgaactcacc aacattcttg
     4441 ccagccgggc caacgcgcgc tcggaaaggc gcgggctacc cgtttcgtgt cgtgccggct
     4501 gcggtgcctg ttgccggcag atggttccgc tgtcgatccc cgaggtgttc tacctgatgg
     4561 agcggatcgc ggagatggag cccgccgcgc gtgacgtggc cgtcaacagg ttcatggcga
     4621 tcgatcgccg gctcgacatg gcggggatgg tggagccgct gctcgacttg aaggtggccg
     4681 ccgatccgca gcgcgcgctc aaccagacct atttcgagat gaagatggcg tgcccgttcc
     4741 tggtggacga atcctgttcg atccacagtg tccgaccggt ggcctgtcgt gaatacagtg
     4801 tcaccaacaa cccgaagttc tgcgcaaacc cgtacgccgg cggcatcgac aaggtgccca
     4861 tgccgctgcc gctctcggcc gcgctggcgt ggctggcctc ggaacgactc ggcgttccgc
     4921 cgatgttgat cccgatgaca ctggtgccgc gatgggcatc ggagaatgcc gagctggcgg
     4981 agcagcgttg gccgggcgtt gagctgtttt cgcagttcat gaagatcgcg gggagcccgc
     5041 cgccggagtg ggcaatgaac ccggacgcac cgccggcggg gaactgacag cttcccgttt
     5101 tcccaccgac ctgtaacgat tagactgccc tcaacaggtt ccggaacctg ttactttgcc
     5161 tcgcaaagtt tcgcggatcg aaccagcgaa cgggcatgag gcccagcacc cgatgtcccc
     5221 aaggaggaag tcccccatgc tcggtcggat ctctttcgcg ctcctcacca tatgcgcgtg
     5281 taccctgacc tccaccgccc gggccgacat tgacgtgttt atcaatttcg accgcgacac
     5341 caacggcatc gccctcgaat cccccgccgt catcaacgag gtgtatctgt cctggggcgt
     5401 cacgttcggc tccaaccccg gcgcccaatg cggcagcggc gaggtctacg ccaccagcgc
     5461 ctgcctcagc gaaccggccc ccacatcggc tcccaacatc gtgagcttct gcgccgggtg
     5521 cagcgatatc tccgaggaca cgggctacgt catcgccacc ttcctgatgc ccgcggacag
     5581 cgtctgcatc accgccatcg ccgccgagcc caacggtcgg agctacctgc gggcctacga
     5641 cgcggagcac aacctggtcg tcgaagacat cagcaatccc gagctggctt cggatgtcct
     5701 gtgcgtgacc ggaccggcga tccacacggt ggaattcacc ggccttccgg gcgagttcgc
     5761 ctggttcgat gacatgcagg tgagcttcgc cgaggtgccg gtcgagccgg tgacatggag
     5821 cggcatcaag gctcgggagc gctgacttcc cgtggggcga cgggcaaccc cacgatcacc
     5881 cactataaag gaggcccgga cagccaattc ccgccctaag ccattgtaat aaaacagatt
     5941 aacggcacaa tcattcaaat cgtcaactta tccgtcacta tcgcagtaca gatataggga
     6001 aatttttgct actctgccgc ccgggtcgaa tccactcgac gcacacgtga tttcgaaacc
     6061 accacaagcg tcatcgaccc atcgagccgc ggcccgcccc cagacacaca cgcgggtcac
     6121 cacatccccg aacaggaggt ccctcatgat ccgtcggcta ctgcccctcg ccgcctgcgc
     6181 tctcgcgctc ggcctcacca ccgccgccca tgccggcatc gacgtcgtca tcaacttcga
     6241 caccgacacc aacggcaacc ccatccccca cggcaccatc gtgaacaatc tctacgcgcc
     6301 ctggggcgtg aacttcgacc gcaccgtccc cggcagctgc ggcagcgaaa gcggcgcgtt
     6361 cgtctttacc agcaacgagt gcctct
//
LOCUS       DASVOD010000371         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_495425, whole genome shotgun sequence.
ACCESSION   DASVOD010000371 DASVOD010000000
VERSION     DASVOD010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495425"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VF720_08550"
     CDS             <1..1337
                     /locus_tag="VF720_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7879445.1"
                     /translation="PDTLFENIHKLRAGHTMGVRAETGPEPPKRFWNPLPKAGAPVIS
                     DPAEGVRLVRETLDAAVEARLMSDVPFGAFLSGGIDSSAIVALMSRHMDRPVDTFSIG
                     YRDDPTFNEYEYARRVADLFKTNHREVLIGHDDFKSFLPKLIYHQDEPISDPVCVPLY
                     YVAKLARDSGVIVTLVGEGSDELFFGYQWHNRIRKMYEGWWRPLSKLPAPVRAGAAGA
                     ASLVVDPPRRDFLRRFAEGGEPFLGGAVTFYDNELSELLDPDLARACEPPAAVVDRLY
                     AETDGILDPTDFTRRSTYLELMYRLPELLLMRVDKMTMATSVEGRVPFLDHSMIELAF
                     RLSADLKVRDGVTKWVLKEAMRDILPAEILHRRKLGFHVPVTAWFGKDLSGWAREVLL
                     DDASAVAGLFRRSAVERLLDRQSKGGVNFGMRIWALVNFALWHRYWIEKKSI"
     gene            1359..2240
                     /locus_tag="VF720_08555"
     CDS             1359..2240
                     /locus_tag="VF720_08555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879446.1"
                     /translation="MDPSLYHRFFEVEDRHWWFVGRRRVVGALMEQALGSPGSGSRSG
                     LPLRAGASSQHSASTADVAGERGGAPPARRILDVGCGTGGMLPLLSRFGHVTGTDSEP
                     LALDYCRRRGFEDVHLQEAFTAPAPFDVVTLFDVLEHVPDHVGFLEWIRKLLKPDGHL
                     VLTVPAFPFLWSRHDDLNHHQRRYKRADLKATLEAGGFRVERLTYFNFWLFPAAVATR
                     ARGGKAESVAGRTTDPAAGRTTVDPAAEAAEILKPLQIGAANPVLAAVLGSEAGLVRQ
                     VDLPWGVSLAAVARPAR"
     gene            complement(2311..>3101)
                     /locus_tag="VF720_08560"
     CDS             complement(2311..>3101)
                     /locus_tag="VF720_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879447.1"
                     /translation="GDNRADAGDLVRLADIVLGRGEPPTSEELELADCNGDDALDIRD
                     MICLSDVIVPSGSAPPPAGNGSLATLQFALEVPVRAIELDFPVGTALEDAADALAPLA
                     VTTWVQANGAVALMAWDPAGRPLPAGSFDAVPASRAPLDARAWGDGGKALSVHRDGRS
                     VSIGAAIEAPPVPVVSAAPTPFNDATTIRWRVSRPGVTTVQIFDVRGALRRSWTSSEL
                     AAGDHAWTWDGRDEAGERLASGVYFARVGIPDGRRTLRLVRVVR"
BASE COUNT          495 a         1072 c         1030 g          504 t
ORIGIN      
        1 cccccgacac ccttttcgag aacattcaca agctccgggc cgggcacacg atgggtgtgc
       61 gcgcggaaac ggggcccgaa ccgccgaagc gtttctggaa cccgctgccg aaggccggtg
      121 caccggtcat ctcggatccc gccgagggcg tgcggctcgt ccgcgaaacg ctcgatgccg
      181 ccgtcgaggc ccgcctcatg agcgacgtac cgttcggcgc cttcctgtcg ggtggcatcg
      241 actccagtgc catcgtggcg ctgatgagcc ggcacatgga ccgtccggtc gacacgttct
      301 ccatcggtta ccgcgatgat ccgacattca acgagtacga gtacgcccgg cgagtagccg
      361 acctcttcaa gaccaatcac cgcgaagtgc tgatcggaca cgacgacttc aagtcgttcc
      421 tgccgaaact gatttaccat caggatgagc ctatttcgga ccccgtctgc gtgccgctct
      481 attacgtggc caaattggcg cgcgactccg gcgtgatcgt gacattggtg ggggagggct
      541 ccgacgagct cttcttcggc taccagtggc acaaccggat ccggaagatg tacgaggggt
      601 ggtggcggcc gctgtcgaaa ctgccggccc cggtgcgggc cggcgcggcc ggcgcggcct
      661 cgctggtggt cgatccgccc cgtcgcgatt tcctgcgccg atttgccgaa gggggagagc
      721 cgttcctcgg cggggccgtc acgttctacg acaatgaact gtccgagctg ctcgatcccg
      781 acctcgcccg cgcctgcgaa ccgccggcgg ctgtcgtcga ccgcctctat gccgaaaccg
      841 acggcatcct cgatcccacc gacttcacgc ggcgcagcac ctatctcgag ctgatgtacc
      901 gcctccccga actgctcctg atgcgcgtgg acaagatgac gatggccacc tcggtggagg
      961 ggcgcgtacc gttcctcgat cactcgatga tcgagctggc cttccgcctg tctgctgacc
     1021 tcaaggtgcg cgacggcgtg accaagtggg tcctgaagga agccatgcgc gacatcctcc
     1081 cggcggagat ccttcaccgc aggaagctcg gctttcacgt tcccgtgacc gcatggttcg
     1141 gcaaggacct gtcaggatgg gcgcgcgaag tcctgctgga cgacgcctcg gcggtggctg
     1201 gcctgttccg ccgttcggcg gtcgagcggt tgctcgaccg ccagtcgaag gggggcgtca
     1261 acttcggcat gcgcatctgg gcgctggtca acttcgcgct ctggcaccgc tattggatcg
     1321 agaagaagtc gatctaggcg atgacggccg cctgactcat ggaccccagc ctctaccacc
     1381 gcttcttcga ggtcgaagac cgccactggt ggttcgtcgg gcgacgccgg gtcgtgggcg
     1441 cgttgatgga gcaggcgctc ggatcacccg ggtccggctc gcgatccggg ttacccttgc
     1501 gagcaggcgc ctcctcgcaa cacagcgcct cgacagccga tgtcgcaggg gagaggggag
     1561 gcgcgcctcc ggcgcgccgc atcctggacg tcgggtgcgg cacgggtggc atgctgccgc
     1621 tcctatcacg cttcgggcat gtcaccggca ccgactctga gccactggcg ctggactatt
     1681 gccgccggcg cggcttcgaa gacgtgcatt tgcaggaagc gttcaccgcg ccggcgccct
     1741 ttgacgtcgt tacgctcttc gacgtcctgg aacatgtgcc cgaccacgta ggcttcctgg
     1801 agtggatccg gaagctcctc aagcccgacg gccaccttgt cctgaccgtg ccggcctttc
     1861 ccttcctctg gagccgccac gacgacctga atcaccatca gcgtcggtac aagcgcgccg
     1921 acctgaaggc gacactggaa gccggcggct tccgcgtcga gcgcctgacc tatttcaatt
     1981 tctggttgtt cccggcggcc gtggcgacgc gcgcgcgggg tggcaaggcg gagtcggtcg
     2041 ccggacgcac gacggatcca gccgcgggaa ggacgaccgt ggacccagcc gcggaagccg
     2101 cggagatcct gaagccgtta cagatcggcg cggccaatcc cgtcctggcg gccgtgctcg
     2161 gttccgaagc ggggctggtc cgccaggtcg atcttccctg gggcgtttcg ctggccgcag
     2221 tcgcgcgacc ggcgcgataa tactagtatg acaatgtaag acaaccgcgc ggatctataa
     2281 ccgcgcggcc ctcccctgtg gaacgcccgc ctaccgcacc acccgcacca accgcagggt
     2341 acgtctccca tccggtatcc cgacccgcgc gaagtagacc cccgacgcca gcctctcgcc
     2401 ggcctcatcg cgcccgtccc aggtccaggc gtggtcacca gcggccagct cggacgaggt
     2461 ccagctgcgg cggagcgcgc cgcgaacgtc gaagatctgc acggtcgtca cgccggggcg
     2521 tgacacccgc caacgaatcg tcgtggcgtc gttgaagggt gtcggtgctg ccgaaaccac
     2581 cgggaccggc ggtgcctcga tcgccgcgcc gatggacacg gaacgaccgt cacggtgaac
     2641 cgaaagagcc ttgccgccat cgccccaggc ccgggcgtcc agcggcgccc gggaagcggg
     2701 caccgcatcg aacgagccgg ccggaagcgg gcgcccggcg ggatcccagg ccatcagggc
     2761 cacggcgccg ttcgcctgca cccacgtcgt gaccgcgagc ggggccagcg catccgcggc
     2821 atcctcgagc gcggtgccga ccgggaagtc gagctcgatg gcccggaccg gtacttcgag
     2881 ggcgaactgg agcgtggcca gggagccgtt gccggcgggc ggcggcgccg atccggacgg
     2941 cacgatcacg tcggacaggc agatcatgtc ccggatgtcg agcgcgtcgt cgccgttgca
     3001 gtcggccagc tcgagctcct cggaggtcgg cggttcgcca cgcccgagaa cgatgtcggc
     3061 caggcgcacg aggtcgccgg cgtcggcacg gttgtcgcca t
//
LOCUS       DASVOD010000372         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_234125, whole genome shotgun sequence.
ACCESSION   DASVOD010000372 DASVOD010000000
VERSION     DASVOD010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234125"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF720_08565"
     CDS             <1..247
                     /locus_tag="VF720_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879448.1"
                     /translation="AAFPVVTLAEILAPLVLLNRRFRLAWIVIIVTFHLVSWGMMQIF
                     FLQNILLMPFLLHEWAPGSPGRTGARPPGTGRLPLST"
     gene            complement(308..775)
                     /locus_tag="VF720_08570"
     CDS             complement(308..775)
                     /locus_tag="VF720_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879449.1"
                     /translation="MSRWTKLTTAALVAAAMTTMAGPGRANVDAEVRGGYYTDAEQGF
                     VGGGFLANIADTWDFNPNLEYVFVDGGSLFTVNADIHKDLNPGSGPAFWLGAGPALIV
                     SNPDVPDADTDTDIGLNLIGGIGAKSGSIRPFGQAKYTIADENELSMALGLRF"
     gene            1018..1818
                     /locus_tag="VF720_08575"
     CDS             1018..1818
                     /locus_tag="VF720_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879450.1"
                     /translation="MTMLPSRFPLVAAFVLLASTGLACGDDSPTQPDVTNDRIGRVLF
                     LGNSLTYTNNLPVMVDSLAAASGIEVQCTAITDGGFGLIDHYASASRRAAVLNGHFDW
                     VILQQGPSSLDESRDSLIAWSELWEPLIKASGAKGAMYAVWPERDRFYAFPAVSESYR
                     LAAERIDALFLPVGDSWLETWARNPNIGLYGRDNFHPAVAGTYVAAVAIVSMLAGRPA
                     ISLSTRYTVPRSIGAPIDSTAAATIRAAVDAVVERNRMVEARPTGRPD"
     gene            1985..>2191
                     /locus_tag="VF720_08580"
     CDS             1985..>2191
                     /locus_tag="VF720_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879451.1"
                     /translation="MNGRRPGLSGAVWLLLALVILSVGTSGCGSKRSVDVSRLRAYQP
                     GGATVLYDKDGRPFADLAPVDRILI"
BASE COUNT          347 a          741 c          715 g          388 t
ORIGIN      
        1 ggcggcgttc cccgtggtga cgctggccga gatcctcgcg ccgctggttc tcctcaaccg
       61 gcgctttcgg ctggcgtgga tcgtcatcat cgtcaccttc cacctcgtgt cctgggggat
      121 gatgcagatc tttttcctgc agaacattct cctgatgccg ttcctccttc acgaatgggc
      181 gcccgggagc ccgggcagga ctggtgcacg accgcccggc acaggccgtt taccgctctc
      241 aacataaaga caagacccgg atcgccaggg cgaaccgggt ctcgtggata cgtgacgttg
      301 aggtcgctta gaagcgcaga ccgagcgcca tcgacaattc gttttcgtcg gcgatcgtgt
      361 acttggcctg gccgaagggg cggatcgaac cgctcttggc gccgatgccg ccgatcaggt
      421 tcaggccgat gtcggtatcg gtgtcggcat ccggcacatc gggattgctc acgatgaggg
      481 ccgggccggc cccgagccag aaggccgggc cgctgccggg gttcaggtcc ttgtggatgt
      541 cggcgttcac ggtgaagagg ctgccgccat ccacgaacac atattcgagg ttgggattga
      601 aatcccacgt gtcggcgatg ttcgccagaa agccgccgcc cacgaagccc tgctccgcat
      661 cggtgtagta accgccgcgc acttcggcgt caacgttggc gcgaccgggg ccggccatgg
      721 tggtcattgc ggcggccacg agggcggccg tcgtgagctt cgtccatcgg gacatgatgc
      781 aatcctccgg gttcattcca ccgagatcga tgcttcctgc gaggcatcgg gatcccgtac
      841 ggcgatggat gatccgcaag ccgcgtgccg ggaacaggct cgcggcggcc aatcggccgt
      901 ggtgcaatgg gatggcgggg tcgggcatcc cgttcggagg acgaaactac cgacgacttt
      961 cggtccaggg tgggaattta cgacttggtg acacgggggg cccgtatcat ggcgcggatg
     1021 acgatgttac ccagccgctt cccgcttgtc gccgcgttcg tccttctggc ctccacgggc
     1081 ctcgcgtgcg gcgacgacag tccgacgcaa cccgacgtca cgaacgatcg catcgggcgc
     1141 gtcctcttcc tcggcaacag tcttacctac accaacaacc tgccggtcat ggtcgactcg
     1201 ctcgccgcgg cctcgggaat cgaggtccag tgcacggcca tcaccgacgg cggcttcggc
     1261 ctcatcgacc actacgcgag cgcctcgcga cgggccgcgg tgctcaacgg gcacttcgac
     1321 tgggtgatcc tgcagcaggg cccctcatcg ttggacgaaa gccgcgattc gctcatcgca
     1381 tggtcggagt tgtgggaacc gctgatcaag gccagcggcg cgaaaggggc catgtatgcg
     1441 gtgtggccgg aacgtgatcg gttctatgcc tttccggcgg tgagcgagtc gtaccgcctg
     1501 gccgccgaaa ggatcgacgc cctcttcctg ccggtggggg attcctggct tgagacgtgg
     1561 gcgcgcaacc cgaacatcgg gctctacggc cgcgacaact tccacccggc ggtggccggc
     1621 acctacgtgg ccgcggtcgc catcgtttcg atgctggccg gccggccggc catctcgctc
     1681 tccacccgtt acacggtgcc ccgctcgatc ggcgcgccga ttgacagcac ggcggccgcc
     1741 accatccgcg ccgcggtgga cgccgtggtc gagcggaacc ggatggtcga ggcacgcccg
     1801 accggccggc cggactgacc ggagaatccc gcgcaacgaa cgggggggcg agaacgtctt
     1861 acacgcgcgg ccccggcacg cccggcggcg cgacgctcgc cggggcgcaa tccggtaaaa
     1921 agtaccggcc cggattttgc aaacactcac tttcatgacc cctcgacatc gaacgctcgg
     1981 atccatgaac ggccgtcgcc ctggcctgtc cggcgcggta tggctcctgc ttgccctcgt
     2041 tatcctctcc gtcgggacat ccgggtgcgg ctcgaagcgc tccgtggatg tcagccgcct
     2101 gcgcgcctac cagccgggcg gcgccaccgt cctctacgac aaggatggcc gcccgttcgc
     2161 cgacctggcg cccgtcgacc gcatcctcat c
//
LOCUS       DASVOD010000373         3230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_912341, whole genome shotgun sequence.
ACCESSION   DASVOD010000373 DASVOD010000000
VERSION     DASVOD010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912341"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VF720_08585"
     CDS             <1..1349
                     /locus_tag="VF720_08585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7879452.1"
                     /translation="PDAPRDSLAARLRLAGPDPAGLRYVFVPDTGAVVFGNQAPRGLR
                     RTLLRELNRPAGTDSLASFGMTMRTPGQGTATSGERPPGDPGGSANARVEGRREGARR
                     GPQWGMKIMARRPVSLADHRSGELLAVSYRPTFPFWPGALSQRALLLLPVTLAVAAVG
                     GLLLFRMIARRLSALETLAGRVAAGEFDARVADTSADEIGQLGARLNEMTARLESARQ
                     RQAAEEVVKRQLLADISHELATPLTAIRTQAETMLDPAVPLTDEERRAYLTGVLDESD
                     RMNRLIQDLLELTRLEAGAAALDLEEIDAVDLARNIVQRFRPRFQSANLTLAWEGPAE
                     PILVRADGRHLEQVVENLLMNGLRHVTTGGTVRVSATRVADRLVLRVTDDGPGLAESD
                     RERIFDRFYRADASRSLPGSGLGLAIAREIVNRLGGAIRADGAGAGTAFVVELPAL"
     gene            1495..2634
                     /locus_tag="VF720_08590"
     CDS             1495..2634
                     /locus_tag="VF720_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879453.1"
                     /translation="MITSHRSTPSWRRLRPAMALGLAGLAALGFAAAGCGDDDAGAGL
                     TGADASLTSELNLDIPALAELNSRLNLSEEQARDMEKVLAAWRSDASERMEKRAGKHE
                     AGARKESKLHAEGARGHGFLMGRMGAGRGFGDGLLADGPGFDHLADAAAILDNDQLAT
                     FTTYIEERRQARRGEWRERADKAPHRDGPMARKMASRFGLSESETEELRAAHKESAER
                     TRQLHGDYAAGTISAEQLRDGLRDSRLAMEAKARELMGAEEFEALHSKGNGKRTEMFE
                     RRSERLDERATKQAERLARALRMDAAGKSRIEAALRETIPARRQLLDELSSDQVTPED
                     ALYRGIQIEKEASAAIRATLSPDEVVRFDSLARLIPGSRMVHGRG"
     gene            complement(2745..>3230)
                     /locus_tag="VF720_08595"
     CDS             complement(2745..>3230)
                     /locus_tag="VF720_08595"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX7879454.1"
                     /translation="PGRGVRGPHVGDAMGLLDKLTEDMKNAMREKDALKTSVLRMTMS
                     ECKYARVEKMRDLTDDEVIAVIKKGVKSREDSETQYRNANRPDLADKEAAEAVILKAY
                     VPATIGGPELEAIVAEAITSTGAASPKDIGKVMKAVLGAHGARVDGKDVQKIAAQKLG
                     A"
BASE COUNT          473 a         1109 c         1157 g          491 t
ORIGIN      
        1 cgccggacgc gccgcgcgat tcacttgcgg cgcggttgcg cctggccggg cccgacccgg
       61 ccggcctgcg ctacgtgttc gtgccggaca cgggggccgt cgtcttcggc aaccaggcgc
      121 cgcgcgggtt gcgccgcacg ctgcttcgcg agctcaatcg accggcggga acggacagcc
      181 tcgcgtcgtt cgggatgacg atgcggacac cgggccaagg gacggcgaca tccggggaac
      241 gccccccggg cgatccgggc ggctccgcga acgctcgcgt ggagggtcgc cgcgagggag
      301 cgcgacgcgg tccgcagtgg gggatgaaga tcatggcgcg ccggccggtg agcctggccg
      361 atcaccgctc cggggagctc ctcgcggtga gctaccgacc gacctttccc ttctggcccg
      421 gcgcgctgtc gcagcgggcc ctcctgctcc tgccggtgac cctggcggtg gcggccgtcg
      481 gcgggctcct cctcttccgg atgatcgcgc ggcgactgtc ggcgctggag acgctggccg
      541 gacgcgtggc ggccggggag ttcgacgccc gcgtggccga caccagcgcg gacgagatcg
      601 gccagctcgg cgcccgcctc aacgagatga ccgcgcgcct cgaatcggcg cggcaacggc
      661 aggcggccga ggaagtggtg aagcggcagc tcctggccga catctcgcac gagctggcca
      721 cgccgctgac ggccatccgc acccaggccg agacgatgct cgacccggcc gtgccgctga
      781 cggacgagga gcgtcgggcc tacctcacgg gcgtgctcga cgaatcggac cggatgaacc
      841 gcctgatcca ggacctgctc gagctgacgc gcctcgaggc cggggccgcc gcgctggatc
      901 tcgaggaaat cgacgcggtg gacctggcgc ggaacatcgt gcagcgcttc cggccccggt
      961 tccagtccgc caacctgacc ctcgcctggg aggggccggc cgaaccgatc ctggtgcggg
     1021 cggatggccg gcacctggag caggtggtcg agaatctcct gatgaacggt ctgcgccacg
     1081 tgacgaccgg ggggaccgtg cgggtgtcgg ccacgcgggt ggcggatcgc ctggtgctcc
     1141 gcgtgacgga cgatggtccg gggctcgccg aatcggatcg cgagcggatc ttcgatcgct
     1201 tctaccgggc ggatgcctcc cgttcgctgc cgggaagcgg gctcggcctc gccatcgcgc
     1261 gggagatcgt caaccgcctc ggcggggcga tccgcgccga cggcgccgga gccgggacgg
     1321 ccttcgtcgt ggaacttccc gccctgtaag caattagagt tcgcccgagc atcaagccgc
     1381 agccatcaaa ctttccaagg ttcctgccac atttctgaca actcctgggc gtactacttc
     1441 tcgcatccgg gcacggagac gtttcggccc ggttcccgga aggagtgcga ttccatgatc
     1501 acgtctcatc gctcgacccc ctcgtggcgg cgacttcgcc cggcaatggc cctcggcctg
     1561 gccggtctcg ccgctctcgg attcgccgcc gccggatgcg gcgacgatga cgcgggcgcg
     1621 ggcctgacgg gcgcggacgc gtccctcacc agcgaactga acctcgatat ccccgcactg
     1681 gccgaactca acagccgcct gaacctgtcc gaggagcagg cccgcgacat ggagaaggtg
     1741 ctcgccgcat ggcgcagcga cgcgagcgag cggatggaga agcgggccgg aaagcacgaa
     1801 gccggcgcgc gcaaggaatc gaagctccat gccgagggcg cccggggtca cggtttcctc
     1861 atgggccgga tgggcgcggg tcgcggcttc ggcgacggcc tcctggccga cggaccgggc
     1921 ttcgatcacc tggcggacgc ggctgccatc ctggacaacg accagctcgc cacgttcacc
     1981 acctacatcg aggagcgccg ccaggcgcgc cgcggcgagt ggcgggaacg cgccgacaag
     2041 gcgccgcacc gggacggccc gatggcccgg aagatggcct cccgtttcgg cctgagcgaa
     2101 tccgaaaccg aggagttgcg ggcggcccac aaggagtcgg ccgagcggac gcgccagctt
     2161 cacggtgact acgccgcggg cacgatctcg gcggagcaac tccgggatgg attgcgcgat
     2221 tcgcggctgg cgatggaagc gaaggccagg gagttgatgg gcgccgagga gttcgaggcg
     2281 ctccattcga aggggaacgg gaagcgcacc gagatgttcg agcgccgctc ggagcgcctc
     2341 gacgaacgcg ccacgaagca ggccgagcgc ctggcccgcg cgctccggat ggacgcggcc
     2401 ggcaagtccc ggatcgaagc ggcccttcgc gagacgatcc cggcccgccg ccagctcctc
     2461 gatgagctgt cgagcgacca ggtgacgcca gaagatgcgc tgtatcgcgg gatccagatc
     2521 gaaaaagagg cgtcggcggc gatccgtgcc acgctgtccc cggacgaagt cgtccggttc
     2581 gacagcctcg cgcggctgat ccccggcagc cggatggtac acgggcgggg ctgatttcgg
     2641 ccgcgttcgc gcgccggcgg agtgggacat cctggagaat gcaggctccg ggctggctgc
     2701 tccgccggtg ttttttttgg tttgattcgg gaggttgccc ggcgtcaggc cccgagcttc
     2761 tgcgcggcga tcttctgcac gtccttgccg tcgacccgcg cgccgtgggc gccgaggacc
     2821 gctttcatca ccttgccgat gtccttcggt gacgcggcgc ccgtcgaggt aatggcctcg
     2881 gccacgatgg cctcgagctc cggcccgcca atggtggcgg gcacgtaggc cttcaggatg
     2941 accgcctccg cggcttcctt gtcggccagg tcgggccggt tcgcgttacg gtactgggtt
     3001 tccgagtcct cgcggctctt gactcccttc ttgatcaccg cgatgacctc atcgtcggtc
     3061 aggtcgcgca tcttctcgac cctggcgtac ttgcattcgg acatcgtcat gcgcaggacg
     3121 gaagtcttga gcgcgtcctt ctcgcgcatg gcgtttttca tgtcctcggt gagcttgtcg
     3181 agcaggccca tggcgtctcc aacgtgaggg ccccggacgc cacgtccggg
//
LOCUS       DASVOD010000374         3723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_598552, whole genome shotgun sequence.
ACCESSION   DASVOD010000374 DASVOD010000000
VERSION     DASVOD010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598552"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF720_08600"
     CDS             <1..422
                     /locus_tag="VF720_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7879455.1"
                     /translation="LTVRPTSLLNECTPPTWRADVMADEDSADVVACSAQLEAAWKDF
                     TTPPAPLPAPGSEGHDILVCHGNVIRWFVTKALKVDTMAWLQMGLCNASLTTIQVRAD
                     GTTRVLGYADNGHLPPNMQTGLNRTSRELVVPGATPR"
     gene            803..2050
                     /locus_tag="VF720_08605"
     CDS             803..2050
                     /locus_tag="VF720_08605"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7879456.1"
                     /translation="MKFRSLCLSTALLLFSVPASARNAFEYAGVRTHGFHLFEGVHAE
                     IGTVNSDDGSTGFLWGINTSAGTIFSPNIDLTLGFRSWSSDLDPSDTGIDGSVSDFSL
                     HGDMSYHFFKVWGVRPYGLAGLATHFVSADVPGDSEREDSVGGFNLGLDFGIGAATTR
                     KGLGVRGEIRREVAGDAGNWSYTVGVGWWPTQNAVRVPTAASVAPASSPPATPAPVGT
                     SGNTSAPTSGGLSMAEAAALQSTIDRLNAENEALKSQSGAAPAAAPKTATPEDRAAAQ
                     RAAFQRVARLAGNPDGYSDGADGPRFVTQSLVTFEEGGSKLDTRDQEQIRRVAVLLMA
                     FPGSSAVIEGHADSGGSVAANQKVTTARAEAVRGELVRLGVPAGSLTALGLGSSRPMF
                     DNASANKLSNRRVSIQVNGATIR"
     gene            2149..>3723
                     /locus_tag="VF720_08610"
     CDS             2149..>3723
                     /locus_tag="VF720_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879457.1"
                     /translation="MRMRASVAMLGLVLVGLAPGAARADTEDGGSGLSRVEHAYALPI
                     GRPLFNLGLGYYSKGDFDSTEARYVTLTPSVTFGLGMGFEGSVALPLDGLTTTEGLDD
                     YERRFDIRASEATAKLRYTGNFFTPRVRFGVMGLADVSLGDSRRAGGSKDPDVGYDPG
                     ILGMLSFNFGPYDVPMRLHLNGGYWQSRNDGAVYFRDFPGAIPVPGLDPDDNDVIEAG
                     AAIEFGFRGATAFAELSTEYLADGGDFMSPSEGYWRFTPGVKFKLSSTIAMTAAISAD
                     VSSDDDETEFQPDDIYPETEFQLALSLGRVFGQAGYETTRKERDDNRKREEEELAAAQ
                     AAAAAAAASRLTGSPVDSASIRAAMRDTVATPRMAPTTASPTAQTPYSVGPMGYPPAG
                     TTESRMMELEYRLRFLEMNLRMSEIEQRLRYMSPPPAYPPQPNYYSAPPDTSRRSEDR
                     SDNLTQPTGTGAAGAAPAATTATSPTPTEMAAADEAARTRAAEAALIAEQQREIDRLQ
                     RELNQSNLSSSQRQQRD"
BASE COUNT          648 a         1263 c         1227 g          585 t
ORIGIN      
        1 atctgacggt gcgccccacg tcgctgctca acgaatgcac gccgccgacg tggcgggccg
       61 atgtcatggc cgacgaggac tcggccgacg tggtggcctg cagcgcccag ctcgaggcgg
      121 cctggaagga cttcaccacg cctcccgccc cgctccccgc gccgggaagc gagggacacg
      181 acatcctcgt gtgccacggc aacgtgatcc gctggttcgt gacgaaggcg ctcaaggtcg
      241 atacgatggc ctggctgcag atgggcctgt gcaacgccag cctcaccacc atccaggtgc
      301 gggcggacgg cacgacgcgc gtgctgggtt acgcggacaa cggccacctg ccccccaaca
      361 tgcagaccgg actcaaccgc acgagccgtg agctggtcgt tccgggcgca acgccgcgct
      421 agtcggcgag cacctcgata gcgccgcaaa gggtgtggcc gttggcgctg gggcagcccg
      481 agggtgcctg gacgcgctct ataatgtagg gattgcatac ggtaaatgga aatattttcc
      541 atataagtga caaatgtcag tctgccttgg atagtgcctc cggaatcatg acgcatggtt
      601 ccgcgcaccg gtgaccatgc cgatcgcccc ttgatgcacg tgtcttccat gcatcgcatt
      661 ggatccggca ctccgaattg tcggagttgc agcatgtggg cgatggcgat cgttcgcatg
      721 gccaccctcg acaacccgaa tcagcgcgga gtacattgcc gaaagtaggc accttttccc
      781 gctgaaagcg agggcggcac tgatgaagtt ccgatcgctt tgtctttcca ccgcacttct
      841 tctgttctca gtccccgcat ccgccaggaa tgccttcgag tatgcgggcg tgcggacgca
      901 cggattccac ctcttcgagg gcgtgcatgc cgagatcggc acggtcaatt ccgacgatgg
      961 atccacggga ttcctgtggg gaatcaatac gagcgccggc accatcttct cgcccaatat
     1021 cgatcttacc ctcggcttcc gcagctggag cagcgatctc gacccgtccg acacgggcat
     1081 tgacggcagc gtgagcgact tcagccttca tggcgacatg agctatcact tcttcaaggt
     1141 gtggggcgtc cggccatatg gcctggccgg cctggccact cacttcgtct cggccgacgt
     1201 tcccggtgac tccgagcgtg aagactcggt cggcgggttc aacctggggc tcgacttcgg
     1261 catcggcgcg gccaccacgc gcaagggact cggtgttcgc ggcgaaatcc gtcgcgaagt
     1321 ggcgggcgat gcgggcaact ggagctacac cgtcggggtc ggctggtggc cgacacagaa
     1381 tgccgtccgc gtcccgacgg ccgcctcggt ggcaccggcc tcctcgccgc cggcaactcc
     1441 cgcgcctgtc ggcacatccg gcaacacgtc ggcaccgacc tccggcgggc tcagcatggc
     1501 tgaagcagct gcgctccaga gcacgatcga ccgcctgaac gccgagaacg aggcgctcaa
     1561 gtcccagtcc ggcgccgcgc cggccgccgc cccgaagacc gcaacgcccg aggaccgcgc
     1621 ggccgcccag cgtgccgcgt tccagcgcgt ggccaggctc gccggtaatc cggacggcta
     1681 ttccgacggc gccgacggac ccaggttcgt cacgcagagt ctcgtcacgt tcgaagaggg
     1741 tggatcgaag ctcgacacgc gcgaccagga gcagatccgg cgcgtggccg tgctgctgat
     1801 ggcgtttccg ggttcgagcg cggtgatcga aggtcatgcc gactcgggcg gcagtgtggc
     1861 cgccaaccag aaggtcacga ccgcccgcgc ggaggccgtg cgtggcgagc tggtgcgtct
     1921 cggcgttccg gccggatcgc tgaccgccct ggggctcgga tcgtcccggc cgatgttcga
     1981 caacgcttcg gccaacaagt tgtccaatcg acgggtgagc atccaggtga acggggccac
     2041 gatccgctga acggaatcgt ggtcggagtc gaggtgcacg aatttcaaac ggagttcctt
     2101 ccaggtggca tcgggtcgcc tggcacgggc tcgaggagat ggccgaacat gcgcatgaga
     2161 gcaagtgtgg caatgctggg cctggtgctg gtcggcctgg cgccgggcgc ggcccgggcc
     2221 gacacggaag acggcggttc gggcctgtcc cgcgtggagc acgcgtacgc ccttccgatc
     2281 gggcggccgc tgttcaacct gggactcggc tactacagca agggggattt cgactcgacg
     2341 gaggcgcgat acgtcacgct cacgccgagc gtgacattcg gcctgggcat ggggttcgaa
     2401 ggctccgtcg cgctgcccct cgacgggctc accaccacgg aaggcctgga cgactacgag
     2461 cgccggttcg acatccgcgc ctcggaagcc accgccaagc tccgctacac cggcaacttc
     2521 ttcacgccgc gcgtacgatt cggcgtgatg gggctggccg acgtgtcgct cggcgacagc
     2581 cgccgcgccg gtggcagcaa ggatccggac gtgggatacg acccgggcat cctcggcatg
     2641 ctctccttca acttcggtcc gtacgacgtg ccgatgcgcc tgcacttgaa cggcggttac
     2701 tggcagagtc gtaatgacgg cgcggtgtac ttccgcgact tcccgggcgc gatcccggtg
     2761 ccgggcctcg accccgatga caacgacgtg atcgaagccg gcgcggccat cgagttcggc
     2821 ttccgcgggg ccaccgcctt cgcggaactc tccaccgaat acctggccga cggcggcgac
     2881 ttcatgtcgc ccagcgaagg ttactggcgc ttcacgccgg gcgtgaagtt caagctctcg
     2941 agcaccatcg cgatgacggc ggccatctcg gccgacgtgt cgtccgacga tgacgaaacc
     3001 gagttccagc cggacgacat ctatcccgaa accgagttcc agctggcgct gagcctcggc
     3061 cgcgtcttcg gtcaggccgg ttacgagacg acgcgcaagg aacgggacga caaccgcaag
     3121 cgggaggagg aagagcttgc cgcggcccag gcggctgccg cggccgcggc ggcttcgcgt
     3181 ctcacgggat cgccggtcga ctcggcgtcc atccgtgcgg cgatgcgcga cacggtggcc
     3241 acgccgagaa tggcaccgac aacggcctcg ccgacggccc agacgcctta ctccgtggga
     3301 ccgatgggtt atccgccggc cggtaccacc gagagccgga tgatggagct ggagtatcgt
     3361 ctccgcttcc tcgagatgaa tctgcgaatg agcgaaatcg agcagcgact ccgttacatg
     3421 tcgccaccgc cggcctaccc gccgcagccg aactactatt cggcgccgcc ggacacgagc
     3481 cgtcgctcgg aggatcgcag cgacaacctg acacaaccaa ccggcacggg ggcggcaggc
     3541 gccgcccccg cggccaccac ggccacgtca ccgacaccga ccgaaatggc cgccgccgac
     3601 gaagcggccc ggacccgggc cgccgaggcg gcgctcatcg ccgagcagca gcgggagatc
     3661 gaccgcctgc agcgcgagct caaccagtcg aacctgtcgt cgtcccagcg gcagcagcga
     3721 gac
//
LOCUS       DASVOD010000375         3871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_808041, whole genome shotgun sequence.
ACCESSION   DASVOD010000375 DASVOD010000000
VERSION     DASVOD010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808041"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VF720_08615"
     CDS             <1..680
                     /locus_tag="VF720_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879458.1"
                     /translation="TKPIRVANANAILSFDEVALVEPGEPGAPWPDPFFYDYVVVEGS
                     KDGCTWTAFQDGWDARAYPEWETAYANNTPSPALFRRRNINMLNAFDVDDTLLVRFRL
                     FADAGANGWGWIIDNLDIQSAAQSGVGDGPSAAVLSLTPGTNPFRDRTTLAYTLPTSG
                     RVALNIFDVSGRLVRQLVDEVKPAGQYEVTWDGSTAGGDRAANGMYFVQLVGADQVVK
                     KKVTLLR"
     gene            complement(750..1910)
                     /locus_tag="VF720_08620"
     CDS             complement(750..1910)
                     /locus_tag="VF720_08620"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7879459.1"
                     /translation="MYERLLRARLERFEQKSVLVLGPRQVGKSTLLRSLGPQLAIDLS
                     SPDVYRRYVGHPERLEKELRAVTAGRQTVLIDEVQRVPALLDMVQALVDESPDRYRFL
                     LSGSSARKLRRGQANLLPGRVLVYAMHPLLQVEIGSMFELDRALGHGTLPGIYGESDP
                     ELRNELLRSYADTYLREEIQAEGIVRDVGGFSRLFDYVAAASGRILNINALSQDAGIS
                     YVTARRYMEILEDTLVLWRIPAWSGSDRGKLVAHPKVILFDLGVRNALLRRPLDKPLP
                     DERGLLLEQFVGLEILCRTGSLWPDARLHHFRTRAGLEVDYLLEVGREVWGIEVKASS
                     SVGRADFRGLNALDTHVRTLKRRIVVFLGDRAQAYDGVEVLPLAEFLSLLPA"
     gene            complement(1939..>3871)
                     /locus_tag="VF720_08625"
     CDS             complement(1939..>3871)
                     /locus_tag="VF720_08625"
                     /inference="COORDINATES: protein motif:HMM:NF036968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879460.1"
                     /translation="IGEKPLTSYVPLEEATKGIVVTQYEMHAIEEIGLVKIDLLGNRA
                     ISVISEAVHLVSESGGPTIVPADIPDGDVPTGELLARGDTLGVFQLESPGMRNLLRQL
                     ETADLDGSIAALSLIRPGPAGSGMKDRFIRRVRGREPIDHLDPRLGPLLESTYGVLLY
                     EEDVMAVGAALAGLSLADGDMLRRAIAEMESEGDEVEVRRVWIERAIRNHTKPDVAEA
                     VWKELRRFGAYAFCKAHASGYGVLAYQGAYLKAHWPAAFAVAILNNHTAMYEQRVHLE
                     DARRHDVGIRLPCINRSGKEYLLEPAHAGDTAPAGAAIRIGLGRIRQLTEATIERTLA
                     GRPFADLADWVRRVRPIFKEAEHAILAGAFDFTGRIRPQLLCELRLTWAAHVRASRSL
                     ATLAAGLATGTDGGLFGALPIDVPMPVLADFDDAERIRQEFRVLELTADRNAMTLFDT
                     AGFLPSRDLYARKGRRVRMAGIVAAERRVKTKRGEWMFFLTLEDEDGLFECTLFPPAW
                     RRYSGILHGVGPYIVEGKAEEQYGAVTVNVERLKAWGLWVVDTIPTSRSNSVRTELPD
                     LFRYQPTVSGNSWHFVSPSIPTSANARRPRLPTRWRGPGMTSRAIPGHRFGLDLPHAL
                     ASLCGRKLFACNGLISFLT"
BASE COUNT          701 a         1275 c         1242 g          653 t
ORIGIN      
        1 tgacgaagcc gatccgcgtg gccaacgcca acgccatcct gtcgttcgac gaggtggcgc
       61 tggtcgagcc cggcgagccg ggcgcgccat ggcccgaccc ctttttctac gactacgtgg
      121 tggtggaagg aagcaaggac ggctgcacct ggaccgcctt ccaggacggc tgggacgccc
      181 gtgcctatcc cgagtgggaa acggcctacg ccaacaacac cccgtcaccg gcactcttcc
      241 ggcgacggaa catcaacatg ctcaatgcct tcgacgtcga cgacacgctg ctggtccgct
      301 tccgcctctt cgccgacgcc ggcgccaacg gctggggctg gatcatcgac aacctcgaca
      361 tccagtcggc ggcccagtcg ggagttggtg acgggccgtc ggccgcggtc ctgtcgctta
      421 caccgggcac caacccattc cgcgaccgga cgacgctggc ctacacgctt ccgacctccg
      481 ggcgggtggc gctcaacatc ttcgacgtga gcgggcgcct cgtgcggcag ctggtggatg
      541 aagtgaagcc ggccggccag tacgaggtca cctgggacgg ctccaccgcc ggcggcgacc
      601 gcgcggccaa cgggatgtac ttcgtgcaac tcgtgggtgc ggaccaggtg gtgaagaaga
      661 aggtgacgtt gttgcggtga ggcgtcggcc tccatcaccc tgttacgaat acagccaaca
      721 ggttccggaa cctgttggct gtttctcctt caggccggta acagcgaaag aaactcagcg
      781 agcggcaaga cctcgacgcc gtcgtaggcc tgcgccctgt caccaagaaa gacgacaatc
      841 cggcgcttga gcgtgcgcac atgcgtgtcc agcgcgttca gacctcgaaa gtcggcccga
      901 ccgaccgacg acgacgcctt cacctctatc ccccacacct cgcgtccgac ctcgaggaga
      961 tagtcaacct cgaggccggc acgagtccgg aaatgatgaa gcctggcgtc gggccacagg
     1021 ctgccagtgc ggcaaagaat ttccagcccg acaaactgtt ccagcaggag cccacgttcg
     1081 tccggcaacg gcttgtcgag tggacgccgc aagagggcgt tgcgaactcc caggtcgaac
     1141 aggatcacct tgggatgagc gaccaacttg ccacgatcgg agccgctcca ggcggggatg
     1201 cgccagagga ccagtgtgtc ctcgaggatt tccatgtagc gccgcgcggt gacgtaggag
     1261 attcccgcat cctggctgag ggcattgatg ttcaggattc tcccggaggc cgcagcaacg
     1321 taatcgaaga gtcgcgagaa acctccgaca tcccgaacta ttccctcggc ctggatttcc
     1381 tcgcgcaggt acgtgtcggc gtagcttcgg agaagttcgt tgcgcaattc cggatcggac
     1441 tcgccataaa ttccaggcag ggtgccgtgc cccagggccc gatccagctc gaacatcgac
     1501 ccgatctcta cctgcagcag cgggtgcatc gcatagacca gcacgcgccc cggaagcaga
     1561 ttggcctgtc cgcgcctcaa tttgcgcgca cttgatcccg acagcaggaa tcgatatcga
     1621 tcgggagatt cgtcaacgag tgcctggacc atgtccagca atgcgggcac ccgctgcacc
     1681 tcgtcgatca ggacagtttg acggccagcg gtgacggccc gcagctcttt ctccaagcgc
     1741 tcgggatgcc cgacgtagcg gcggtacacg tcgggactcg acaggtcgat ggccaactgc
     1801 ggccccagcg accgaagcaa cgtcgatttg ccgacctggc gcggcccgag gaccaggaca
     1861 ctcttctgct caaacctttc cagccgggca cgcagcaatc gttcatacat gtggttaaaa
     1921 ataccgcatt tttgtccact atgttaagaa acttattaaa ccattgcatg caaatagctt
     1981 acgcccacat agagatgcta gagcgtgcgg caggtcaagt ccgaaccggt gacccggtat
     2041 ggcgcgactc gtcatgcccg gtccgcgcca cctcgtcggc aagcgtggcc ttcgagcgtt
     2101 agcactcgtt gggatggatg ggctcacgaa gtgccaactg tttccggaaa cagttggctg
     2161 atatcggaac aggtccggca actcggtccg gaccgagttg gaacgtgatg tcggaatagt
     2221 gtcgacaacc cacaaccccc acgccttcag cctctccacg ttcaccgtca cggccccgta
     2281 ctgctcctcg gccttccctt ccacgatgta cggccccacg ccgtggagga tgccgctgta
     2341 gcgccgccag gccggcggga acaacgtgca ctcgaacagg ccgtcttcgt cttcgagcgt
     2401 gaggaagaac atccactcgc cgcgcttcgt cttgacgcgc cgttcggcgg ccacgatgcc
     2461 ggccatccgg acgcggcgcc ccttccgggc gtagaggtcg cggctcggca ggaagccggc
     2521 ggtgtcgaac agcgtcatgg cgttccggtc ggcagtgagc tcgaggacac ggaactcctg
     2581 gcggatccgc tcggcgtcgt cgaagtcggc caggaccggc atcggcacgt cgatggggag
     2641 ggcgccgaag aggccgccgt cggtgccggt ggcgagtccg gcggccagcg tcgcgaggga
     2701 gcgcgaggcc cggacatgcg ccgcccaggt gagtcgcagc tcgcacaaca actgcggccg
     2761 tatacgcccg gtgaaatcga acgcgccggc caggatggca tgctcggctt ccttgaagat
     2821 gggccgcacg cggcgcaccc agtcggcgag gtcggcgaac ggccggccgg ccagggtgcg
     2881 ctcgatggtg gcttcggtga gctggcggat gcggccgagg cctattcgta tcgccgcgcc
     2941 ggcaggcgcg gtgtcgccgg cgtgagccgg ctccaacaga tattcctttc cgctccggtt
     3001 gatgcacggc agccgtatcc ccacatcgtg ccgccgcgca tcctccaggt gcacccgttg
     3061 ctcgtacatc gccgtgtgat tattgaggat ggccaccgcg aacgcggccg gccagtgcgc
     3121 cttgaggtac gccccctggt aggccagcac gccgtaaccc gaggcgtgcg ccttgcagaa
     3181 agcgtaggcg ccgaagcggc gcagttcctt ccagaccgcc tcggccacgt cgggcttcgt
     3241 gtggttccgg atggcgcgct cgatccagac gcggcgcacc tcgacttcgt ccccttcgct
     3301 ctccatttcg gcgatggccc ggcggagcat gtcgccgtcg gccagtgaga gaccggcgag
     3361 cgcggcgccg acggccatca catcctcctc gtagagaagc acgccgtacg tcgattcgag
     3421 cagcggtcca agcctgggat cgagatggtc gatcggctcg cgcccgcgca cgcggcggat
     3481 gaagcggtct ttcattcccg aaccggcggg gccgggacgg atgagcgaga gcgccgcgat
     3541 cgagccgtcg aggtcggcgg tttcgagctg cctcaggaga ttccgcatgc cgggcgattc
     3601 gagctggaag acgccgagcg tgtcgccgcg ggccagcaac tcaccggtgg gcacatcgcc
     3661 atccgggatg tcggcgggga cgatcgtcgg gccgcccgat tctgatacga gatgcaccgc
     3721 ctccgagatc accgagatgg cccggttgcc gagcaggtcg atcttgacca ggccgatctc
     3781 ctcgatggcg tgcatttcgt actgcgtgac gacgatcccc ttggtggcct cctcgagggg
     3841 cacgtacgac gtgagcggct tctcgccgat g
//
LOCUS       DASVOD010000376         7948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_287019, whole genome shotgun sequence.
ACCESSION   DASVOD010000376 DASVOD010000000
VERSION     DASVOD010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287019"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VF720_08630"
     CDS             complement(<1..419)
                     /locus_tag="VF720_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879461.1"
                     /translation="MRRILPTLLLSLFVVAGCSSDDDDTNPTLPPAAEAEVAMVEAGA
                     SVAGEIFASMVSRLPFLALPGTPPSEGIVWGPDNGPGVPPNSYVFVLPVDVDDDGDPD
                     SQLNGHCVLSGDPSTVGPGFNGTIQVSGTTPGNIGDFT"
     gene            731..1393
                     /locus_tag="VF720_08635"
     CDS             731..1393
                     /locus_tag="VF720_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879462.1"
                     /translation="MSARFILMAALVAAGLVGAIPAGAQLVNPAFDAGPAGPVGNFGP
                     VVGPPFATGFWGAEAADIVVEDICGLPPRSNPFMLQLNVGGGAYSQAWQAVDVSPTPP
                     LQVSFHGWANTCSLAPGVTVGLDIRTFNNPNGWPTHTLITSTNLVLDTDPATWQRVSL
                     NCVTIPADTYWILAQVFLVNATSQVPAYFDDMELVFDECPTPVEPTTWSRVKALARVE
                     TP"
     gene            complement(1486..2508)
                     /locus_tag="VF720_08640"
     CDS             complement(1486..2508)
                     /locus_tag="VF720_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879463.1"
                     /translation="MRDPRDRSPKSFDHFKRDNRAPNDRQAGRPDDRARNPQSPDRNA
                     PQGQRPDDRRGSYPPRGPSQGQRPDNRQGSYPDRGGPRGARPDNRQGGYPDRGGARGQ
                     RPDDRRGGYPDRGGSQGPRPDQRGRNDHQGRPDQPRNDSRRYDPRGQDSRGQAPHGQG
                     QRRPDHRQPDSRRADPRPHDSRRPDQRNDQRAYQQGPANPRPLPPPQADANRPAHLQQ
                     QGRAGDPTPFELFCAYHLGIQKDGTYKPANIHTIAKQFNRPSGELKQMLQNYGLDPMS
                     VMERDFDLAMAQIDMQLAPEGINKIELARPIFEEFLAAKRKKIDWKKIIEEDIRENAK
                     VFGRRD"
     gene            complement(2667..4706)
                     /locus_tag="VF720_08645"
     CDS             complement(2667..4706)
                     /locus_tag="VF720_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7879464.1"
                     /translation="MLTLRTILVPAAGLLLAAAPVSAQTFTTVFSPTIGSHVSFSTGA
                     TWVDVDDDGDLDIYVVTGFAANNDNVLWRNNGDGTFDSIFPLPITSDAAESACGVFAD
                     RDNDGDVDGFVSNLVNAGGMLFDGATGGGTPMNPGGFTLNTTAGSGGPGLKGTGAAWG
                     DYDNDGHLDLVIAALFNQGGITTANRLFHNNGDGTFSEVTTDPVVTITDSHHHPTWAD
                     YDGDGDIDLFFGTGGPGFAKLDRLYRNQLKETGTATFTPITSGVIATDTRDSQLLSWV
                     DYDNDGDLDMYAVNYSSVPNQLYRNDGGTFTKITTGDLVTGVGANHGVVWGDFDNDGD
                     PDAYVARDSRQSNRYFRNNGNGTFTRITAGPHVNEALSNYGAAAGDYDRDGDLDLFVP
                     TARSETASQLFRNDTVNGNHWITLRCRGTISNRSGVGAVVRVKAVIDGAPRWQRRDIA
                     TGTSYGGSDALEAHFGLGTAAVIESLRVDWPSGLCDVRAGVAVDQYLTIVEDSSTPVA
                     PSLVSAVVANDIVHVEWRLQEGSRGFVVQRALDASPFEAWRSASPDADGRVVVTDDDT
                     RPGRRQGYRLVHADGTTSAAAWVTVEARLALTIVGSPRRGGTISLLLSSIGGAPAEVE
                     LLDVAGRRRRYQRIEGDAAGQREVALDGNGLPAGVYFVQVRQGGRDVGARLVLTP"
     gene            complement(4817..5557)
                     /locus_tag="VF720_08650"
     CDS             complement(4817..5557)
                     /locus_tag="VF720_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7879465.1"
                     /translation="MITTVLVDDEPLARAGLRELLSAQADISIVGEAGDGDAAVDLIA
                     DSRPDLVFLDIQMPGRSGLDVVRAVSPRHLPAIVFVTAHDRFALEAFEVDALDYLLKP
                     PSAERLHASLDRVRRELATDRPRAEALAGLLDRTEPGARAAGRLVIRDRGRYLLLPHE
                     EIDWIGASGNYVELHSGDRSHLHRETMAALEARLDPGRFRRIHRSTLVNLDRIRSIAP
                     DGAGDFQVTLTDGTTLRMSRRFRERVLK"
     gene            complement(5554..6717)
                     /locus_tag="VF720_08655"
     CDS             complement(5554..6717)
                     /locus_tag="VF720_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7879466.1"
                     /translation="MTEPGPGFPRPRLWLAVLGVAVLCGLLEGAQGWFQYHDLEPGRF
                     WPEAIARAMPSWLILALLFPFVHWVAFRFPVDGRHWRRSLPVHFLAGTTFVVVHLGGA
                     AWVSALRHVTAHTWSEAMLGFVTRYLVTDYAVYGMLVGSIQVWHQWSEIRRRAAVEER
                     LRADLAEARLAALRHQLSPHFLFNTLNSLSALALTGDRDTLVRAIDALSGFLRATLEE
                     APGTTTRFDAELAVLDRYLEIQDLRFGDRLMVERDIARETAAVRVPPLLLQPLVENAI
                     SHGVAARIGPGKVSIRACREDDHLVVVVTDSGPGFGAGNGGAAGFGGGAAGFGGGGGR
                     GDTNGGGRGHGLGLANTRARLAHLFADRASLACDDAPTGGARVTIRLPWDETS"
     gene            6829..7785
                     /locus_tag="VF720_08660"
     CDS             6829..7785
                     /locus_tag="VF720_08660"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX7879467.1"
                     /translation="MDLGTTYLGFKLSNPFMPGASPLVDDLDMVRRLEDAGASAIVMH
                     SLFQEQHPGSAGGSSSGRGATPATDFALDPAQYLEQIRRIKGAVAVPVIASLNGTMVD
                     SWLEWARSIEQAGADALELNIYYLAADPLESSAEVDRRVVHIARRVRAMVSIPVAVKL
                     SVFISGVAHLANELDTLGIQGLVLFNRFYQPDIDIETRRVVPRLRLSDSSELLVRLRW
                     TASLSPQVRCGLAVSGGVHTVEDAVKALMAGASAIQVVSCLLQRGPEYLVPLREGLAA
                     WLEANGHDRLDGIQGSLNHRLSPDPGEFERANYTRILQGPRN"
BASE COUNT         1223 a         2673 c         2767 g         1285 t
ORIGIN      
        1 gtgaagtcgc cgatgttgcc gggagtggta ccgctcacct ggatcgtgcc gttgaagccg
       61 gggccgacgg tcgagggatc gccgctgagc acgcagtggc cgttcaactg cgagtcggga
      121 tcgccgtcgt cgtccacgtc caccggcagg acgaagacgt agctgttggg aggtacgcca
      181 gggccgttgt ccgggcccca cacgatccct tcggacgggg gcgttcccgg gagggccagg
      241 aacggcaggc gcgacaccat cgaggcgaag atctcgccgg ccacgctggc gccggcctcg
      301 accatcgcga cctcggcctc ggctgcgggg ggtagggtgg gattcgtatc gtcgtcgtcg
      361 ctcgaacagc cggcgacgac gaacagtgac agcaagagag ttggaagaat gcggcgcatg
      421 actcctccaa tcggggggtg tccggtgaca tcgaaacagg tgcctccggc acgcgcgaga
      481 ggctgccggg aggggaccgt gaggctagcc cgcccggcgg gggatcacaa gtcggccgga
      541 cagattccga aatagattgt tcgcgccggt ccgacccgcc tatattggcc gggcaccggg
      601 tacgggatcg gggccggaga ggcccgcctc gagtcccgcc cacggaaggt cgcgggggcg
      661 ttgcctctcc atcctctccg gcgacaggaa ggaccgttcc atgcagttgg tagcgcgtgc
      721 acggaagtcg atgtccgccc gtttcatcct catggccgcg ctcgtcgcgg ccggactcgt
      781 cggcgccatc ccggccgggg cccagctcgt gaaccctgca ttcgacgcag ggccggccgg
      841 tccggtcggc aacttcggtc ccgtggtcgg tccgccgttc gcgacgggtt tctggggcgc
      901 cgaggcggcc gacatcgtgg tggaggacat ctgcgggctg ccgccgcgca gcaacccctt
      961 catgctccag ctcaacgtgg ggggcggcgc gtacagccag gcgtggcagg cggtggacgt
     1021 gtctcccacc ccgcccctcc aggtcagctt tcacggctgg gccaatacgt gcagcctcgc
     1081 gccgggagtc acggtcggcc tcgacatccg gaccttcaac aatccgaacg gctggcccac
     1141 gcacacgctg atcaccagca ccaacctggt cctcgacacg gatcccgcca cctggcagcg
     1201 ggtcagcctg aactgcgtga cgatcccggc cgacacctac tggatcctgg cgcaggtgtt
     1261 cctggtcaat gccacgtcgc aggtgcccgc ctatttcgac gacatggaac tcgtgttcga
     1321 cgagtgcccc acgccggtcg agccgaccac ctggagccgg gtgaaggccc tggcgcgagt
     1381 cgagacgccc tgaggcgcgg aatcgaagcg tattgaggtc cacaaataaa aaccgggcga
     1441 tggctcatcg accatcgccc ggttgctttt gaccctgttt gcgtctcagt cgcgccggcc
     1501 gaacaccttg gcgttctcgc ggatgtcttc ctcgatgatc ttcttccagt cgatcttctt
     1561 gcgcttggcg gccaggaatt cctcgaagat cggcctggcc agctcgatct tgttgatccc
     1621 ctcgggcgcg agctgcatgt cgatctgtgc catggccagg tcgaaatcac gctccatgac
     1681 gctcatcggg tcgagcccgt aattctggag catctgcttg agctcgcccg acggccggtt
     1741 gaactgcttc gcaatggtgt ggatgttggc cggcttgtac gtaccgtcct tctggatgcc
     1801 gaggtgatac gcgcagaaga gctcgaacgg cgtgggatcg ccggcgcgtc cctgctgctg
     1861 caggtgcgcg gggcggttcg cgtcggcctg gggcggcggc agcggccgcg gattggccgg
     1921 gccctgctgg taggcgcgct ggtcgttgcg ttggtcgggt cgcctcgaat cgtgcggacg
     1981 cgggtcggcc cgccgtgaat ccggctggcg atggtcgggt cgccgctgac cctggccgtg
     2041 tggggcctgt ccgcgcgagt cctgaccacg ggggtcgtag cggcgcgagt cgttgcgtgg
     2101 ttggtcgggg cgcccctggt gatcattgcg cccgcgttga tccgggcgtg gcccctgcga
     2161 tccgccgcgg tcgggatagc cgccgcgccg gtcgtccggc cgttgtccgc gagccccgcc
     2221 gcgatccggg tagcccccct gccgattgtc cgggcgcgcc ccgcggggtc cgccgcgatc
     2281 gggatacgaa ccctggcgat tgtccgggcg ctgtccctgc gacgggccgc gcgggggata
     2341 cgagccccgg cgatcatcgg ggcgctgtcc ctgcggcgcg tttcgatccg gcgattgggg
     2401 attgcgggcg cgatcgtccg gacgtccggc ctgccggtcg ttgggcgctc gattgtcgcg
     2461 tttgaagtga tcgaagctct tgggtgaacg gtctctcggg tcgcgcatgc aactaggcta
     2521 actgccggag cgatgaatcg caaccgaggg actgtttcca ggaacagtgg gctgtctttg
     2581 atacagggcg tcgaactatg ttcgggcaga gtaggggcta tgtccaggca gagttgagct
     2641 cggtccaggc agagctccgg cgagcatcag ggggtcagca cgaggcgcgc gcccacatct
     2701 cgaccgccct gtcgtacctg tacaaaatag acgccggccg gcaatccatt tccgtcgaga
     2761 gccacctccc gctgaccggc ggcatctccc tcgatgcgct ggtatcgccg ccgccgaccc
     2821 gccacatcca gcagttcgac ctcggccggt gccccgccga tcgaggacag gagcaatgaa
     2881 atcgtcccgc cgcgacgcgg cgatccgacg atcgtcaggg ccagccgggc ctccaccgtg
     2941 acccacgcgg cggcggacgt cgtgccgtcg gcgtgcacca ggcgatatcc ctggcggcgg
     3001 ccggggcgcg tgtcgtcgtc ggtcacgacg acacggccgt cggcatcggg cgaggcgctc
     3061 cgccaggcct cgaaagggga tgcgtcgagg gcgcgctgca cgacgaatcc cctcgatccc
     3121 tcctgcaggc gccactcgac gtgaacgatg tcgttggcca cgacggccga gacgaggctt
     3181 ggcgccaccg gcgtggagga gtcctccacg atcgtgaggt actggtcgac cgccacgccc
     3241 gcccgcacgt cgcacaggcc gctcggccag tcgacccgga gtgactcgat gaccgcggcg
     3301 gtgccgagtc cgaagtgggc ctcgagggcg tccgagccgc cgtagctggt gccggtggcg
     3361 atgtcgcggc gctgccatcg gggggcccca tcgatgaccg ccttcacgcg gaccacggcg
     3421 ccgacgcccg aacggttgga gatcgtgccg cggcatcgca gggtgatcca gtgatttccg
     3481 ttcacggtgt cgttgcggaa gagctggctg gccgtctcgc tgcgcgccgt cgggacgaag
     3541 aggtcgagat cgccgtcgcg gtcgtaatcg ccggccgccg cgccgtagtt cgagagtgct
     3601 tcgttgacgt gcggccccgc cgtgatccgc gtgaaggtgc cgttgccgtt gtttcggaaa
     3661 tagcggttcg actggcggct gtcgcgtgcc acgtaggcgt ccgggtcgcc gtcgttgtcg
     3721 aaatcgcccc acaccacgcc gtgattggct cccacgccgg tcaccaggtc gcccgtcgtg
     3781 attttcgtga aggtgccgcc gtcgttccgg tagagctggt tcggcacgct cgaatagttc
     3841 accgcgtaca tgtcgaggtc gccgtcgttg tcgtaatcca cccacgagag cagctgcgag
     3901 tcgcgcgtgt cggtggcgat gacgccgctg gtgatcggcg tgaacgtcgc cgtgccggtt
     3961 tccttgagtt gatttcgata caggcgatcg agcttggcga agccgggtcc gccggtgccg
     4021 aagaagaggt cgatgtcccc gtcgccgtcg taatcggccc aggtcgggtg atggtggctg
     4081 tcggtgatcg tcaccacggg gtcggtcgtc acctcgctga aggtgccgtc gccattgttg
     4141 tggaacaggc gattggccgt ggtgatcccc ccctggttga aaagggccgc gatcaccagg
     4201 tcgagatgcc cgtcgttgtc gtaatcgccc cacgcggcgc ccgtcccctt gagccccggg
     4261 ccgccgctgc cggccgtcgt gttcagcgtg aagccgcccg ggttcatcgg ggtgccaccg
     4321 ccggtggcgc cgtcgaacaa catgccgccc gcattgacga ggttcgacac gaagccgtcg
     4381 acatcgccgt cgttgtcccg gtcggcaaaa acgccacagg ccgactcggc cgcgtccgag
     4441 gtgatcggga gcgggaagat cgagtcgaac gtgccgtcgc cgttgttgcg ccagaggacg
     4501 ttgtcgttgt tggcggcgaa cccggtcacc acgtagatgt cgaggtcgcc gtcgtcatcc
     4561 acgtccaccc aggtggcgcc ggtcgagaat gatacgtgcg agccgatggt gggggagaag
     4621 acggtcgtga acgtctgggc tgagacaggt gcggcggcga gcaggaggcc ggcggccggc
     4681 accaggatgg ttcgaagggt cagcatgccg ggagtctggc ctctgcccgg gccgcccgct
     4741 cctgctttgt gacgagcggc ctcccggagg ggatgaacgc ccgggccgga cgtccagccg
     4801 gtaacggcgg agccccctac ttcagtaccc gctcgcgaaa ccgccggctc atccggagcg
     4861 tcgtgccatc cgtcagcgtc acctggaaat cccccgcacc atccggcgcg atcgaccgga
     4921 tccggtcgag gttcaccagc gtggaacggt ggatcctccg gaagcggccg ggatcgaggc
     4981 gggcctcgag tgccgccatc gtctcccgat gaaggtggga acggtcgccg gaatggagct
     5041 ccacgtaatt cccgctggcg ccgatccagt cgatctcctc atgcgggaga agcaggtaac
     5101 gaccgcgatc ccggatgacg agccgtcccg cggcgcgcgc ccccggttcg gtgcggtcga
     5161 gcagccccgc gagggcctcc gcccgcggtc gatcggtggc cagttcccgc cgcacgcgat
     5221 cgagcgacgc gtgaaggcgc tcggccgagg gcggcttcag gaggtaatcc agcgcatcca
     5281 cctcgaaggc ctccagggcg aaccgatcat gcgcggtcac gaagacgatg gcgggaaggt
     5341 gccgcgggct gacggcccgc acgacgtcca ggccgctccg ccccggcatc tggatatcga
     5401 ggaagacaag gtccggccgt gaatcggcga tcaggtcgac cgcggcatcc ccgtcgcccg
     5461 cctcgccgac gatcgagatg tcggcctgtg cggacaacag ttcgcgcagg ccggcccgtg
     5521 ccaggggttc gtcatcgacc aggacagtcg tgatcacgac gtttcatccc acggcaaccg
     5581 gatcgtgaca cgggccccgc cggtgggagc atcgtcgcag gcaagcgaag cccggtcggc
     5641 aaagaggtgc gcgagacggg cgcgtgtatt ggccaggccg aggccgtggc cgcggccgcc
     5701 gccattggta tcaccgcggc cgccaccacc gccgaagcct gccgcgccac cgccgaagcc
     5761 tgccgcgccg ccgttcccag ccccgaaccc cggccctgag tcggtcacga cgaccacgag
     5821 atgatcgtcc tcccggcagg cgcggatcga aaccttgccg ggcccgatcc gcgcggccac
     5881 gccgtgcgaa atggcgttct ccaccagggg ctgaagcagg agcggcggca cgcgcaccgc
     5941 ggccgtctcc cgcgcgatgt cccgctccac catgagtcgg tcgccgaagc gaagatcctg
     6001 gatttccaga tagcgatcaa gcacggccag ctccgcatcg aaccgcgtgg tcgtgccggg
     6061 cgcttcctcg agcgtggcgc ggagaaagcc gctcagcgcg tcgatcgccc ggaccaacgt
     6121 gtcacgatcg ccggtcaggg ccagcgccga cagcgaattc agcgtattga agagaaagtg
     6181 cgggctcaac tggtgtcgca gcgcggccag ccgggcctcg gccaggtcgg cgcggagacg
     6241 ttcctcgacg gcggcccgcc ggcggatctc cgaccactgg tgccacacct ggatgctgcc
     6301 gaccagcatt ccgtacacgg cgtagtcggt gacgaggtag cgggtgacga agccgagcat
     6361 cgcctcggac catgtgtggg ccgtcacgtg ccgcaacgcc gacacccagg ccgcgccgcc
     6421 gaggtgcacc accacgaagg tcgtgccggc caggaagtgc accggcagcg atcgccgcca
     6481 gtgacgcccg tccaccggaa accggaacgc cacccagtgg acgaacggaa agaggagggc
     6541 caggatgagc cacgacggca tggcccgggc gatcgcctcc ggccagaagc gccccggctc
     6601 gagatcgtgg tactggaacc acccctgtgc cccctcgagc agaccgcaga ggacggccac
     6661 gccgagaacc gccagccaga ggcggggacg gggaaatcct gggccgggtt cggtcatgcg
     6721 ggcagtgtag gcggggttcg ccggcgcggc cacccggatt cggacgcccc tgttcgtgac
     6781 gtggcgcgcc tgccatcatc cggctgcagc gcatcgcccg gaggagacat ggatctcggc
     6841 accacctatc tcggattcaa gctgtcgaat cccttcatgc ccggcgcctc gcccctggtg
     6901 gacgatctcg acatggtgcg gcgcctggag gatgccgggg cgtcggccat cgtcatgcac
     6961 tcgctgttcc aggaacagca tcccggttcc gccggcggat cgtcgtcggg tcggggcgcc
     7021 acgcccgcca cggacttcgc gctcgacccc gcccagtacc tcgagcagat ccgccgcatc
     7081 aagggcgcgg tggccgtgcc ggtcatcgca tcgctcaacg gcacgatggt cgattcgtgg
     7141 ctcgaatggg cccgctcaat cgaacaggcc ggggcggatg cgctcgaact caacatctac
     7201 tacctggccg cggatcccct ggagagcagc gccgaggtcg atcgccgcgt ggtgcacatc
     7261 gcccggcggg tgcgcgcgat ggtgtccatt ccggtggcgg tcaagttgtc ggtcttcatt
     7321 tccggcgtgg cccacctggc caacgagctc gacacgctcg gcatccaggg gctcgtcctc
     7381 ttcaaccgct tctaccagcc cgacatcgac atcgaaacgc gtcgcgtggt gccgcgactc
     7441 cgcctctccg actcatccga gctgctcgtc cggctccgct ggacggccag cctctcgccg
     7501 caagtgaggt gcgggctggc cgtgagcggc ggcgtgcaca ccgtggaaga tgcggtaaag
     7561 gccttgatgg ccggcgcctc ggccatccag gtggtgtcgt gcctcctgca gcggggtccc
     7621 gagtacctgg ttccgctgcg cgagggtctg gcggcgtggc tcgaagccaa cggccatgac
     7681 cgactggatg ggatccaggg atccctcaat caccgcctct ctcccgaccc cggcgaattc
     7741 gagcgggcca actacacgcg catcctgcaa ggtccgcgaa actagcgctg ggcctggtag
     7801 agcgtggcat ccccgccgtg gcagtcggtg cagaagaggg ccgactcggc ccccttgatg
     7861 tcgatcgggt gccggaacgg ttcattccgc acccgcccgg tggggggctc gtccacgaag
     7921 ttctgcgaga ggagcacgtg gcacgagt
//
LOCUS       DASVOD010000377         8427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_27365, whole genome shotgun sequence.
ACCESSION   DASVOD010000377 DASVOD010000000
VERSION     DASVOD010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27365"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2352)
                     /gene="rnr"
                     /locus_tag="VF720_08665"
     CDS             complement(<1..2352)
                     /gene="rnr"
                     /locus_tag="VF720_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX7879468.1"
                     /translation="MPLPSRADLIRHLEEQASHPEKPKEMARALGVKPHEYHAFKEML
                     RELEDSGEIYRVRKGRYGPPDRLNMVVGLLRVVKSGHGYIVPEPGGEEVFVHAAGMNT
                     ALHGDKVVVRIERMRRRSGVGRPGTPGGAEGSVVKVLERARSEIVGTANVGRTHVVVA
                     PDDPRIARDIIIPLDETRGVLDRQKVVAEVLSWGTPHSAPVGRIIEVLGSPGDPGLDI
                     LTIIRGHGLPDRFPEEVEAAAGSIPHAIPAEEIARREDFRDRFTVTIDPVDAKDFDDA
                     LSLRALEGETWELGVHIADVSHYVTSGDTIDLEAYRRATSVYLVDRVIPMLPEHLSNE
                     MCSLKPQVDRLAMSVVMTVDAECEILDARVTDTVIHSRHRLTYQQVQAMFEGDAVLRQ
                     EYGGALDTLESLRRLAKKLSARRAARGSLDFDLPESRVLLDKDGFPMDIQRVVRLESH
                     RLIEEFMVLANEVVARELKRRRIPGMFRVHEPPDPARIEAMLEVITPMGHRLKQGRDG
                     EVTPRALQALLAKAEGRPEENLVNTLVLRSMKRARYDPRPLGHYGLASPDYLHFTSPI
                     RRYPDLVVHRALREAAGTARAPVRKREEWEVRLAQMAVHCSEREQVAEEAERDSVEVK
                     KAEFMQRHLGDEFEGTISGVTSFGLFVSLSDYAVEGLIHVSNLGDDYYIFREDLYALV
                     GESTKRMFRLGDVLTVQVIAVKKELRQIDFGLVARAGEKVPMARSDDLSAPPRSRARS
                     GPRVQAVPRTSTGMGASGRKGPRAKSKTTGRSRAAKGAARARST"
     gene            complement(2353..2769)
                     /locus_tag="VF720_08670"
     CDS             complement(2353..2769)
                     /locus_tag="VF720_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014203421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879469.1"
                     /translation="MEWNRTSSGVENSPTTPTIHVDELREIIRESIDEALGLSDIRLA
                     PRWQAGTVILKPGDPSLKEKEIPLEVFFHKIVMVRDRLRVLEQKINAHKTLGDADKVE
                     MQQYVTKIYGTLTSFNVLFRDRDDQFVGERGGRDDD"
     gene            complement(2825..3685)
                     /locus_tag="VF720_08675"
     CDS             complement(2825..3685)
                     /locus_tag="VF720_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879470.1"
                     /translation="MRTTNSHLCGRPLRYIPPILIMGLLMLSSPGRTDETAVTGVSAQ
                     SAATGVSTEARPIVDALYVAMGGRLAMDALADLRFTFTYTSNDTLRSSRTHWWDRANG
                     RYRIQGKSRTGEEYVILFNTNTQEGTAWVEGKLQEGESQARWLKRGYGTFINDMYWLL
                     MPFKLDDPGVTVVTDGEADVAGAHCDRLKVTFDRVGLTPGDTYWAYVDRANHRMIRWG
                     FILQDDAGPNATESLWDWTEWREVAGVLVAPARVAVADPDRTVIRFENLEGGVGWPDS
                     VFTSTAPIQL"
     gene            complement(3781..4257)
                     /locus_tag="VF720_08680"
     CDS             complement(3781..4257)
                     /locus_tag="VF720_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019090030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879471.1"
                     /translation="MFRTSLRGVAAFGLALVLATASLSPGLAEAGNRRYKSHPKKGKG
                     NQRVVERHVYHETSNHYYDNHDYHGHSHGSSVGAFLGGVAVGAIIGTHANSYCAPPPP
                     PPPPPRRVWVYDCGPCGYHNRSYDGWVSHLTVVHGVPRCDIAVSYPQNYCGHWEGW"
     gene            4560..5699
                     /locus_tag="VF720_08685"
     CDS             4560..5699
                     /locus_tag="VF720_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7879472.1"
                     /translation="MDFSLSDDQKQLEAVAREFARDVIAPKARAHDDSGEFPLDICRQ
                     AWELGLMNVHIPEAYGGLGLTSLEGCLIAEELSAACSGIYTAIEANVLAEAPLLHAAT
                     DEQKKTWLAPMTAEFRLASYAVTEPDAGSDVSGIKTRAIRHGDDYVINGNKMWITGAG
                     RASWFFVLAYTDPAARYQGMTGFVVPADLPGVRVGKKEENMGQRASDTRAVTFEDVTI
                     PAANRIGAEGAGWSIAMSAFDHTRPPVASGAVGVARTALEHAVRYAQERRTFGKPIAQ
                     HQAIAFMIADMAKEVAAARHLVRHAAWLIDNGHRNTMEAAFAKCFAADTAMKVTTDAV
                     QVFGGYGFSKEYPVEKLMRDAKVFQIYEGTSQVQRLIMAREIFKG"
     gene            complement(5743..>8427)
                     /locus_tag="VF720_08690"
     CDS             complement(5743..>8427)
                     /locus_tag="VF720_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX7879473.1"
                     /translation="LEAEWGWGIAFGSATLLFTALNLWWARRAVSRGSRLPGFGGKVA
                     AGLVLFFAAQAGFGFGARHWPAILLWQHRQPFGLVEPIFGKDAGFYVFTLPLAERIGG
                     WALAILFLNAIAVTISYLTGAAGGLGLAPTGIPGQWKVVRRDPVVSATTHWNRLLTHL
                     SILGVLVFLVFAAQAQLAAWGMMFSAQGVVHGPGWTDVHVRLPAYRILIGALVLGALL
                     FVRAAVAHTLKSTVQAILAGVGLAAVVWLVGLNFLPGLNQRYRVSPNEPTLETPFMRH
                     NMGFTRHAFGLTDDDVETRDFPQIVPPRAETLVTDSLTLANMRLWDWRALESTYDQNQ
                     SFRQYYDFYDVDIDRYRVGGATSQVMLALREMNQGAFSENAVTWVNLRMVYTHGYGLC
                     LNPTNEFTPEGLPNYWVRDIPPVSTHPALAITRPEIYYGELTKGPVYVKTGQKEFNYP
                     EGDQNAYTTYEGTGGIRLGSGLRRLALALRLDGIRQLTSADLTAESRLMLRRQVHERV
                     RALAPFLRLDEDPYAVIIDGRLGIVFDGYTVSDHFPYSERLQGGLNYIRNSVKILVDC
                     YDGSVTLYAVDEVDPVLKAWRAAFPTLFQPASAMPAGLRAHLRYPEDLLNLQAHVYGT
                     YHMTDPMVFYNKEDRWAVALESIGQNQPQEMLPYYAVLRLPGESTTEFVQLIPFTPFT
                     ARGAPRNNMIGWMAGRCDGEHYGKLLVYRFPKQSLVYGPMQIEARIDQDAVIAKDLTL
                     WNQQGSNVIRGNLLVVPLENGLLYTKPIFLQATHSRMPELKRVVVASQERLGYGASYA
                     EALADLIEQPLPGSLYRAVTGRWPEGVVTPAIADSGAVALPGGISAMPMDRANLVEAR
                     RLYRRYLELMGAGRAGEAGAVLEELGRTLGSRAGSAEN"
BASE COUNT         1401 a         2857 c         2750 g         1419 t
ORIGIN      
        1 ggtacttcgt gctcttgccg cgcccttagc cgcgcgggac cgcccggtcg ttttcgattt
       61 ggcccgcggc cccttgcggc cactcgcgcc cattccggtg gaggttcggg gaacagcctg
      121 gacacggggc ccggacctgg cacgcgagcg cgggggcgcc gacagatcat cactccgagc
      181 catgggcacc ttctcacccg cccgtgccac caggccgaaa tcgatctggc gcagctcctt
      241 cttcaccgcg atcacctgca cggtgaggac atcgcccagc cggaacatcc gcttggtcga
      301 ctccccgacc agggcataga ggtcctcgcg gaaaatgtag tagtcgtccc ccaggttgct
      361 cacgtggatg agcccctcca cggcgtagtc gctgagggaa acgaagaggc cgaatgacgt
      421 cacgccgctg atcgtcccct cgaactcgtc accgaggtgt cgctgcatga attcggcctt
      481 cttcacttcc acggaatcgc gttcggcctc ctcggccacc tgttcgcgct ccgagcagtg
      541 cacggccatc tgcgcgaggc gcacctccca ttcctcgcgc ttgcgaaccg gcgccctggc
      601 ggtgccggcg gcttcccgca atgcccggtg cacgaccagg tcgggataac gccggatcgg
      661 cgaggtgaag tggagatagt ccggactggc caggccatag tggccgaggg gccgcgggtc
      721 atagcgagcg cgtttcatgc tgcgcagcac gagcgtgttg accaggtttt cctccggccg
      781 cccttccgcc ttggccagga gcgcctggag ggcgcggggc gtcacctcgc cgtcccgccc
      841 ctgcttgagg cggtggccca tcggcgtgat cacctcgagc atggcctcga tcctggccgg
      901 gtcgggtggc tcgtgcacgc ggaacatccc cgggatgcgg cgccgcttca gttcccgcgc
      961 caccacttcg ttggccagca ccatgaactc ctcgatcagc cgatggctct ccaggcgcac
     1021 cacccgctgg atgtccatcg gaaagccgtc cttgtcgagg agcacccggc tctccgggag
     1081 gtcgaagtcg aggctccccc gggccgcccg ccgggccgac agcttcttcg cgagccggcg
     1141 caggctctcg agcgtgtcga gcgcaccgcc gtactcctgc cgcagcaccg catccccttc
     1201 gaacatggcc tgtacctgct ggtacgtcag gcggtgccgg ctgtggatca ccgtgtccgt
     1261 cacgcgggca tccaggatct cgcactccgc gtcgaccgtc atcaccacgc tcatggccag
     1321 gcgatccacc tgcggcttca ggctgcacat ctcgttggag aggtgttccg gcagcatggg
     1381 tatgacgcga tccaccaggt agaccgaggt ggcacgccgg taggcctcca ggtcgatcgt
     1441 atcgccggaa gtcacgtagt gactcacgtc ggcgatgtgc acgccgagct cccacgtctc
     1501 cccctcgagg gcgcgaagcg agagggcgtc gtcgaagtcc ttggcatcca ccgggtcgat
     1561 cgtcaccgtg aagcggtcgc ggaaatcctc gcgcctcgcg atctcttcgg ccggaatggc
     1621 gtgcggaatc gacccggccg ccgcctcgac ctcttcggga aagcggtccg ggagtccatg
     1681 tcccctgatg atggtcagga tgtcgaggcc cgggtcgccg ggcgatccca gcacctcgat
     1741 gatccgcccc accggcgccg aatggggtgt tccccacgag aggacctcgg ccaccacttt
     1801 ctgccggtcg agcactcccc tcgtttcgtc cagcgggatg atgatgtccc gggcgatgcg
     1861 cggatcgtcc ggcgccacca ccacgtgcgt gcggccgacg ttggccgttc cgacgatctc
     1921 gctgcgggcc cgctcgagca ccttgaccac cgagccttcg gcgccgccag gcgtgccggg
     1981 tcgtccgacg ccgctgcggc ggcgcatccg ctcgatccgc accaccacct tgtcgccgtg
     2041 gagggccgtg ttcattccgg ccgcgtggac gaacacctcc tccccacctg gctccggaac
     2101 gatgtagccg tggccgctct tcaccacccg gagcaacccg accaccatgt tcagccggtc
     2161 gggcggcccg taacgcccct tgcggacccg gtagatctcg ccggagtctt ccagctcacg
     2221 gagcatctcc ttgaaggcgt ggtattcgtg cggcttcaca ccgagcgcgc gggccatctc
     2281 cttgggcttc tccgggtggg aagcctgctc ttcgagatgc cggatcaggt cggcgcggct
     2341 gggcagcggc attcagtcgt cgtcccgccc gccccgctcg cccacgaact ggtcgtcgcg
     2401 atcgcggaac agcacgttga aactggtcag ggtgccgtag atcttggtca cgtattgctg
     2461 catctccacc ttgtcggcat caccgagcgt cttgtgggcg ttgatcttct gttcgagcac
     2521 gcggagtcga tcgcgcacca tgacgatctt gtggaagaat acctccaggg ggatctcctt
     2581 ctccttcagt gacggatcgc cgggcttcag gatgaccgtg ccggcctgcc agcgaggtgc
     2641 cagccggatg tcggagagtc cgagtgcctc gtcgatcgat tcgcggatga tctcgcgcag
     2701 ctcgtcgacg tgaatcgtcg gcgtcgtcgg cgaattctcc acgccggatg acgtgcggtt
     2761 ccattccatg tgaattcctc caggtcccga tcacgggccg gatttcaggg tagcggccgc
     2821 ccgctcaaag ttgaatcggg gcggttgagg tgaagacgga atcgggccag ccgacgccgc
     2881 cttccagatt ctcgaaccgg atcacggtgc ggtccgggtc ggccacggcc acgcgggcgg
     2941 gcgccacgag caccccggcc acctcgcgcc attcggtcca gtcccagagc gattcggtgg
     3001 cgttcggccc ggcgtcatcc tgcagaatga acccccaccg gatcatccga tgattcgccc
     3061 ggtcgacata cgcccagtac gtatcgccgg gtgtgagccc gacacggtcg aacgtgacct
     3121 tgaggcggtc gcagtgcgcc ccggccacgt cggcttcgcc gtcggtcacg accgtgacac
     3181 ccggatcgtc cagcttgaac ggcatgagga gccagtacat gtcgttgatg aaggtgccgt
     3241 agccgcgctt gagccacctg gcctgcgact ccccctcctg gagtttcccc tccacccagg
     3301 cagtgccttc ctgggtgttc gtgttgaaga ggatgacgta ttcctcaccg gtgcggctct
     3361 tcccctggat gcgatagcgc ccattcgcgc gatcccacca gtgggtgcgg ctcgatcgga
     3421 gcgtgtcgtt gctcgtgtag gtgaaggtga agcggaggtc ggccagggcg tccattgcga
     3481 gccgcccccc catggcgaca tacagggcgt cgacgatggg gcgggcttcg gtggacacgc
     3541 cggtcgcggc cgattgggcg gacacgcccg ttaccgccgt ttcgtcggtt cgacccggcg
     3601 aactcaacat gaggaggccc atgatcagga tgggggggat gtaacggagc gggcgtccgc
     3661 agaggtggga attcgtggtg cgcatgggtt cgactatagg cgcatcccgc gccaaaaacc
     3721 aaccgcggcc gcgccggtcc cacgtccggc gcgaccgcga tcgatcactc agacgttaca
     3781 tcaccacccc tcccagtggc cgcaatagtt ctgtggataa ctgaccgcga tatcgcaccg
     3841 ggggacgccg tgcaccaccg tgaggtggct cacccatccg tcatagctcc gattgtgata
     3901 tccgcacggg ccacagtcgt agacccatac gcgacgcggc gggggcggcg ggggtggcgg
     3961 gggcgcacag tacgaattgg cgtgcgtgcc gataatggcc ccgacggcca cgccacccag
     4021 gaaggcgccc acactcgagc cgtgtgaatg tccatggtag tcatgattgt cgtaatagtg
     4081 attcgacgtc tcatgataga catggcgttc cacgactcgc tggttgccct tccctttctt
     4141 cgggtggctc ttgtaccgcc ggtttcccgc ctccgcgagg cctggcgaca gtgaggcagt
     4201 cgccaggacc agggcgaggc cgaatgcggc caccccccgc agagaggttc tgaacatgtc
     4261 ccaatcctcc tttctctcga gttccggaca tcccgtcggg actgtccccg gaatggccgg
     4321 tggatccgga ccgcacactg cagccgccgt gccggaggcc gggctcggga gagacccggg
     4381 aaagcgctgc ccaagaccaa gtgtttcaag gttttgcagc cgcgatgtaa gatcgtctcg
     4441 gcctcttgcc ggctcctgcc gataatggct ggagcgcggt tgcgccagtt gtgtcgcgac
     4501 cgggccacgg gcccggccac gactaccacg atccgaaaag ccacggggaa gatcacgtga
     4561 tcgatttttc gctctccgac gaccagaagc agcttgaagc cgtggcccgc gagttcgcgc
     4621 gcgacgtgat cgcgccgaag gcccgggccc atgacgactc gggcgaattc ccgctcgaca
     4681 tctgtcgcca ggcctgggag ctgggcctga tgaatgtcca catcccggag gcgtacgggg
     4741 gcctcgggct gacgagcctc gaggggtgcc tcatcgccga ggagctgtcg gccgcctgca
     4801 gcggcatcta cacggccatc gaggccaacg tgctggccga ggcgcccctg ctccatgcgg
     4861 ccaccgacga gcagaagaag acatggctgg cgcccatgac ggccgagttc cggctggcct
     4921 cctacgccgt caccgagccg gatgccggca gcgacgtctc cggcatcaag acgagggcca
     4981 tccgccacgg tgatgactac gtcatcaacg ggaacaagat gtggatcacc ggcgccgggc
     5041 gcgcctcctg gttcttcgtg ctggcctata ccgatccggc cgcccgctac caggggatga
     5101 ccggcttcgt cgtgccggcc gatctccccg gcgtgcgcgt cggcaagaag gaagagaaca
     5161 tggggcagcg ggccagcgac acgcgcgccg tcaccttcga ggatgtcacc atcccggccg
     5221 ccaaccgcat cggagccgag ggagcgggat ggtcgatcgc catgagcgcc ttcgaccaca
     5281 cgcggccgcc ggtggcctcc ggcgcggtcg gcgtggcgcg caccgcgctc gagcatgcgg
     5341 tccgatatgc ccaggagcgg aggaccttcg gcaagccgat cgcccagcac caggccatcg
     5401 ccttcatgat cgccgacatg gccaaggagg tcgccgcggc ccgccatctg gtgcggcatg
     5461 ccgcgtggct gatagacaac ggccacagga acacgatgga ggcggccttc gccaagtgct
     5521 tcgcggccga caccgccatg aaggtgacga cggacgccgt gcaggtgttc ggcggctacg
     5581 gattcagcaa ggaatatccc gtcgagaagc tgatgcgcga cgccaaggtc ttccagatct
     5641 acgaagggac cagccaggtg cagcggctga tcatggcccg ggagatcttc aaggggtagc
     5701 gcccgtcacg accgcccgct cgacggatga ctcccccgcg gactagttct ccgccgatcc
     5761 ggcgcgcgat ccgagcgtcc ggccaagctc ctcgaggacc gcgccggctt cgccggcccg
     5821 gccggctccc atgagctcca ggtagcggcg gtagagccgg cgcgcctcca ccaggttggc
     5881 tcgatccatc ggcatggcgg aaatcccgcc gggcagggcc acggcgccgc tgtcggcgat
     5941 agcaggggtt acgacccctt ccggccagcg accggtcacc gcccggtaga gcgaccccgg
     6001 cagcggctgc tcgatgaggt cggccaatgc ctccgcgtac gaggcgccgt agccgaggcg
     6061 ctcctggctg gccaccacca cgcgcttgag ctcgggcatc ctcgaatgcg ttgcctgcag
     6121 gaagatcggc ttggtgtaaa gcagcccgtt ctcgagcggc accaccagta ggtttccgcg
     6181 aatgacgttc gacccctgct ggttccagag cgtcaggtcc ttggcgatga cggcgtcctg
     6241 gtcgatgcgc gcctcgatct gcatcgggcc gtacaccagg ctctgcttcg gaaagcggta
     6301 gaccagcagc ttgccgtagt gctcgccatc gcaccggccg gccatccagc cgatcatgtt
     6361 gttccggggc gccccgcgcg ccgtgaacgg ggtgaacggg atcagctgca cgaactcggt
     6421 ggtcgattcc ccggggagtc gcagcaccgc gtagtaaggg agcatctcct ggggctggtt
     6481 ctggccgatc gactcgagcg ccaccgccca gcgatcctcc ttgttgtaga agaccatcgg
     6541 atcggtcatg tggtacgtgc cgtacacgtg cgcctgcagg ttgagcagat cctccgggta
     6601 gcgcaggtgg gcccggagcc cggccggcat ggccgaagcc ggctggaaca gcgtgggaaa
     6661 cgccgcgcgc caggccttca acaccggatc cacttcgtcc accgcataaa gggtcaccga
     6721 gccgtcgtaa cagtcgacga ggatcttgac cgagttgcgg atgtagttga gtcctccctg
     6781 cagcctctcc gaatacggga agtggtcgct caccgtatag ccgtcgaaaa cgatccccag
     6841 ccggccgtcg atgatgaccg cgtacggatc ctcgtcgagg cgaaggaagg gcgccagagc
     6901 ccggacgcgt tcgtgcacct ggcgccggag catcagccgg ctctcggccg tcaggtcggc
     6961 ggaggtgagc tgccgtatgc cgtcgagccg cagggccaac gccaggcgcc ggagtcccga
     7021 cccgagccgg atgccgcccg tgccctcata cgtggtgtag gcgttctggt ctccctccgg
     7081 gtagttgaac tccttctggc ccgtctttac gtacacgggc cccttcgtca actcgccgta
     7141 gtagatctct ggtcgtgtga tcgccagcgc cgggtgcgtg ctgacggggg gaatgtcacg
     7201 cacccagtag ttcggcagtc cctccggcgt gaactcattg gtggggttca ggcacagacc
     7261 atagccatgc gtgtatacca ttcgcaggtt cacccatgtg accgcgttct cgctgaatgc
     7321 cccctggttc atctcccgga gcgccagcat cacctggctc gtggcgcccc ccacgcggta
     7381 tcggtcgatg tcgacgtcgt agaagtcata gtactggcgg aacgactggt tctggtcgta
     7441 cgtggattcg agggcccgcc aatcccataa acgcatgttc gccagtgtca ggctgtccgt
     7501 aaccagcgtc tcggcccggg gagggacgat ctgcgggaaa tcgcgcgttt cgacatcgtc
     7561 gtcggtcagg ccgaaggcat gacgcgtgaa acccatgttg tggcgcatga acggcgtttc
     7621 gagcgtcggc tcgttggggc tgacgcggta gcgttgattg aggccgggca ggaaattcaa
     7681 ccccaccagc cagacgactg ccgccagccc cacgccggcc aggatggcct ggaccgtcga
     7741 cttgagcgtg tgcgccaccg ccgcccgcac gaagagcaag gctccaagca cgagcgcgcc
     7801 gatcaggatt cggtaggccg gcaaccggac atgaacgtcg gtccacccgg ggccgtggac
     7861 caccccctgc gcggagaaca tcatccccca tgcggccagc tgcgcctgcg cggcgaacac
     7921 gagaaaaacg aggacgccga ggatcgagag atgggtcaac agacggttcc agtgcgtcgt
     7981 ggcgctgacg accgggtccc tgcgcaccac cttccattgt ccgggaatcc cggtcggtgc
     8041 aagcccgagc ccgccggcag ctccggtaag gtaagagatc gtgaccgcga tggcattcag
     8101 gaagaggatg gcgagggccc acccgccgat ccgctcggcg aggggaagcg tgaagacgta
     8161 gaaccccgca tccttcccga agatcggctc gacgagcccg aacggctggc ggtgctgcca
     8221 gagaagaatg gcgggccagt gccgggcgcc gaacccgaaa ccggcctggg cggcaaagaa
     8281 gaggacgagg cctgccgcca cctttccgcc gaagcccggc agccgggaac cgcgggagac
     8341 ggcgcgccgt gcccaccaca ggttcaacgc ggtgaagagg agcgttgccg atccgaaggc
     8401 aattccccag ccccactccg cttcgag
//
LOCUS       DASVOD010000378         4903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_182919, whole genome shotgun sequence.
ACCESSION   DASVOD010000378 DASVOD010000000
VERSION     DASVOD010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182919"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF720_08695"
     CDS             <1..703
                     /locus_tag="VF720_08695"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7879474.1"
                     /translation="EVVINNGWGGVLAHECFGHSLEGDGIRKKTSVRATQLGQQVAAT
                     MVEIFDDSTVPNGRGSFTVDDEGTPSQKSHVVEKGILKGYLWDRLNSKLAGYTSSGNG
                     RRNSYRDYPIPRMTNTYIGPGTSTPEEIIASVKDGLYCKDLGGGSVDPASGNFSFFVT
                     EGYQIENGKLAGAVTNTTLTGNATDAMMNIVMCGNDLEIDKVSGNCGKEGQWKPVGVG
                     QPTLKFSEITVGGAA"
     gene            700..2076
                     /locus_tag="VF720_08700"
     CDS             700..2076
                     /locus_tag="VF720_08700"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7879475.1"
                     /translation="MKSDKELRELVQSAVAATIKAGADQADAMLEYSRVFTGRVRDGK
                     PDLVKQATRNGIGIRAFVGGRTGFVSSSDLRPESLAMLTQRAVALAKIGEVDPHAGLP
                     EIPINAAIPVVDLDIFDDALAGLATEKKLAMARATEEAARAFDKRVTKVDGATFSDTL
                     TTITIANSAGRLDQYRATSANIGTRAICDDKNGKQQSGGYGTNRRFLSELESPEKVGT
                     EAAKAAVEMLGPVTLSTRKVPVLMHADIAAAWLGAMVGAFNGEQVFKKSSYLTEKMGQ
                     KIGSDLVTIVDDGVMKRGPVTAPFDGEGLPTRRNVLLDKGVVTTFLYDSYNARKAGTR
                     STGSAQRGYDSTPGIGTRNLHVVNGASTISDMLKAYPALFYMRDQGAFGYTPTTGDYS
                     YQAAGLWIENGEVVHPVDGITVASNTLTMLAGIVKVGNDLNIQGGTNSPHLLIDEMTI
                     SGEGEQAG"
     gene            2073..2540
                     /locus_tag="VF720_08705"
     CDS             2073..2540
                     /locus_tag="VF720_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HEX7879476.1"
                     /translation="MSQTTKRLEWDRYFLKICRVVAERSTCTRARVGAVIVRNRMILT
                     TGYNGSPAGHPHCTDVGCLVVTSRNADGLDEENCIRTIHAEINAIAQAAKNGVSIDGA
                     DIYITHSPCMHCLKVLLNTGLKRICFERPYKLDRMAWLLEPSPVELVQVVMEE"
     gene            complement(2718..3986)
                     /locus_tag="VF720_08710"
     CDS             complement(2718..3986)
                     /locus_tag="VF720_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879477.1"
                     /translation="MTRVRVMQLSALAVTIAAPATWGAVSADIAHLPLRRPVMLVGER
                     RARAVIDEDSLLAAGAAPEFVDVRLMNAQGEAIALAAEPLRRMDEQYRQLRNVPLRWQ
                     EEANGPRRMVIDLGPDPPAELILAFEGPVANHSIAVEGSADSVGWSSMTQRYDLTMPP
                     PKDNRLNTQRIALAETRRWLRVRQDPPYNAPTVDDRLDLMLRLERSTPRVAVGYQATS
                     GFERSGGYRWEARLTLSGPARALTRLDVSGAEAVRSQLGFELQARLPKGGWQYVALER
                     GPEWKGGANDSLLFAPVRTTELRVVVTGGDAPNPPVALHSLWATPVRWTFDIGSGDAW
                     WLAWGDPFLAPAAGGLTMPVTTTNGADPATISPTRLGAVEPNPFHREPGFGLEWLRRR
                     PIVLSVAMVAILALVAWLARRPKGRSGAAA"
     gene            complement(3983..>4903)
                     /locus_tag="VF720_08715"
     CDS             complement(3983..>4903)
                     /locus_tag="VF720_08715"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX7879478.1"
                     /translation="PLSAMPAARGWGVARALVLVANGFLYEAALYDLLGPDFHPLRGV
                     ATAILALDYVMLARAYGARLGPDEGAVKMTRLTGLGLAALAVPIQFDLAAVTLGWSLL
                     ALILMHGGFGRAGLGERLLAYGVLLLAVGHSLLWDTASAFRDLSTYRPWFNGAFLVGL
                     AAAGALGAAAWSISRHRDQLTSGERRLVTPLVLMSGVVLLVRISVEVIAGYELRETLT
                     RGDLYLAMLLTLSLVWAVYAGALIAGGFAFRYRPIRFLGIAVLAMLIVKVFIFDLQEL
                     DRGYRIASFVGVGLLLLMISILYQRERKTA"
BASE COUNT          944 a         1681 c         1584 g          694 t
ORIGIN      
        1 cgaggtggtc atcaacaacg gctggggagg cgtgctggcc cacgaatgct tcggtcacag
       61 cctcgaggga gacggcatcc gcaagaaaac ctcggtgcgg gccacccagc tcggccagca
      121 ggtggcggcc accatggtcg agatcttcga cgactccacg gtccccaacg gccgcggctc
      181 gttcacggtg gacgacgagg ggacgccgtc gcagaaatct cacgtcgtgg agaaggggat
      241 cctgaagggg tacctctggg accgcctcaa ctcgaagctg gccggctaca cctccagcgg
      301 caacggccgg cgaaattcct accgcgacta cccgatcccc cgcatgacca acacctacat
      361 cgggccgggc acctccacgc cggaggagat catcgcctcg gtgaaggacg gcctctactg
      421 caaggacctg ggcggcggtt cggtcgatcc ggccagcggc aacttctcgt tcttcgtcac
      481 cgagggatac cagatcgaga acggcaagct ggccggcgcg gtgaccaaca ccacgctcac
      541 cggcaacgcc accgacgcca tgatgaacat cgtgatgtgc ggaaacgacc tcgagatcga
      601 caaggtctcg ggcaactgcg gcaaggaagg acagtggaag ccggtcggcg tcgggcagcc
      661 cacgctcaag ttctccgaga tcacggtggg aggtgcggca tgaagtcgga caaggaactg
      721 cgcgaactgg tgcagtcggc ggtggcggcc acgatcaagg ccggcgccga ccaggccgac
      781 gccatgctcg aatacagccg cgtcttcacc ggacgcgtcc gtgacggcaa gcccgacctg
      841 gtcaagcagg ccacccggaa cggcatcggc atccgggcct tcgtgggggg gcgcaccggg
      901 ttcgtgtcga gctccgacct gcgtccggag agcctggcca tgctcaccca gcgcgcggtg
      961 gccctggcga agatcggcga ggtggatccg catgccggcc tcccggagat tccgatcaac
     1021 gcggcgattc ctgtcgttga cctcgacatc ttcgacgacg ccctggcggg cctcgccacc
     1081 gagaagaagc tggccatggc ccgcgccacc gaggaagcgg ccagggcgtt cgacaagcgc
     1141 gtcaccaagg tggacggcgc cacgttctcg gacacgctga ccaccatcac catcgccaat
     1201 tcagccgggc gcctcgacca gtaccgcgcc acctccgcca acatcggcac gcgtgccatc
     1261 tgcgacgaca agaacggcaa gcagcagtcc ggtggctacg gcacgaaccg acgcttcctc
     1321 tcggagctcg aatcgcccga gaaggtgggc accgaggcgg cgaaggcggc cgtggagatg
     1381 ctcggcccgg taaccctctc cacgcgcaag gtgccggtgc tcatgcatgc cgatatcgcg
     1441 gccgcctggc tgggcgcgat ggtcggcgcc ttcaacggcg agcaggtctt caagaagtcg
     1501 tcttacctga ccgagaagat ggggcagaag atcggttcgg acctcgtgac gatcgtggat
     1561 gacggcgtga tgaagcgcgg ccccgtaacc gccccgttcg acggtgaggg gctgccgacg
     1621 cgccgcaacg tgctgctcga caagggcgtg gtgacgacgt tcctgtacga ctcgtacaac
     1681 gcccgaaagg ccgggaccag gtcgaccggc agcgcccagc gcggctacga ctcgacgccc
     1741 ggcatcggca cgcgcaacct gcatgtggtg aacggcgcct cgacgatttc cgacatgctg
     1801 aaggcctacc cggcgttgtt ttacatgcgc gaccagggag ccttcggcta cacgcccacc
     1861 accggcgact actcgtacca ggccgccggc ctctggatcg agaacggtga agtggtgcac
     1921 ccggtggacg gcatcacggt ggcctccaac acgctcacca tgctggccgg catcgtgaag
     1981 gtcggcaacg acctgaatat ccagggcggc acgaactcac ctcacctcct gatcgacgag
     2041 atgacgatct cgggcgaagg ggaacaggcc ggttgagcca gaccaccaaa cgcctcgagt
     2101 gggaccgcta tttcctgaag atctgtcgcg tggtggccga gcgctccacc tgcacgcgcg
     2161 cgcgcgtggg cgcggtcatc gtccgcaacc ggatgatcct cacgacgggg tacaacggct
     2221 ccccggcggg tcatccccac tgtaccgacg tggggtgcct ggtggtgacc agccgcaacg
     2281 ccgacgggtt ggatgaggag aattgcatcc ggacgatcca cgcggagatc aacgccatcg
     2341 cacaagccgc caagaacggc gtgtcgatcg acggggcgga tatctacatc acgcacagcc
     2401 cgtgcatgca ttgcttgaag gtgctgctca atacggggct gaagcggatc tgcttcgagc
     2461 ggccgtacaa gctcgatcgc atggcgtggc tgctcgagcc gagtccggtg gagctggtgc
     2521 aggtggtgat ggaggagtag gtggtgccgg cagtgttgcg ctggcgcgac accctggcgg
     2581 taacacgatt cgccagttgt cttacattgt aagacacggt gcgagtcgat tgaccgcacg
     2641 gccgtcgacc ggtctggcca ctgactcacg aacctgtctc gaattcagcc aactgtttcc
     2701 cgaaacagtt gaaatcgtta cgcggccgcc ccggaacgcc ccttcggtcg ccgcgccaac
     2761 cacgccacga gcgccaggat cgccaccatc gccacggaca gcacgatggg ccgccgcctc
     2821 agccactcga gtccgaagcc cggctcgcgg tggaaggggt ttggctctac ggcgccgagc
     2881 cgcgtgggtg agatggtggc gggatcggcg ccgttcgtcg tcgtgaccgg catggtgagg
     2941 ccgccggccg cgggcgccag gaagggatca ccccaggcca gccaccacgc gtcgcctgat
     3001 ccgatatcga atgtccagcg gaccggcgtg gcccacagcg aatgcaatgc caccggggga
     3061 ttgggcgcgt cgccaccggt caccacgacc cgaagctccg tcgtgcgcac cggcgcgaag
     3121 aggagcgaat cgttcgcgcc acccttccac tcgggccccc gttccagcgc cacgtactgc
     3181 caccccccct tggggaggcg ggcctgcagc tcgaagccga gctggcttcg cacggcttcg
     3241 gcacctgaaa cgtcgaggcg cgtgagcgcg cgggcaggtc cggacagcgt gagtcgggcc
     3301 tcccagcggt agccacccga gcgctcgaag ccgctcgtgg cctggtatcc caccgccacg
     3361 cgtggtgtgg accgttcgag ccgcaacatc aggtcgagcc gatcatccac ggtgggggcg
     3421 ttgtagggcg ggtcctggcg cacgcgcagc cagcgccgcg tttcggccag cgcgatgcgc
     3481 tgcgtgttca accgattgtc cttcggcggc ggcatggtca ggtcgtaacg ctgggtcatc
     3541 gaactccacc cgaccgagtc ggcgctacct tccacggcaa tcgagtgatt ggccaccggc
     3601 ccctcgaacg ccaggatcag ctcggccggc ggatcgggcc ccaggtcgat caccatccgc
     3661 cgtggcccgt ttgcctcttc ctgccagcgc agcggcacgt tccgaagttg gcgatactgc
     3721 tcatccatcc ggcgaagagg ctcggcagca agcgcgatcg cctcgccctg cgcattcatc
     3781 aaccgcacgt cgacgaactc cggagccgct cccgccgcca ggagcgagtc ctcatcgatc
     3841 accgcacgcg cccggcgctc gcccacgagc atgaccggcc gccggagcgg caaatgcgcg
     3901 atatcggccg aaacggcgcc ccacgttgcc ggagccgcga tcgtgacggc caatgccgac
     3961 agttgcatca cccgcacgcg tgtcatgccg ttttcctttc gcgctggtac aggatcgaga
     4021 tcatgaggag gagcagtccc acgcccacga acgaggcgat gcgatatccg cggtcgagct
     4081 cctgcagatc gaaaatgaac accttcacga tgagcatggc caggaccgca atcccgagaa
     4141 agcggatcgg acgataccgg aacgcaaacc cgcctgcgat cagcgccccg gcatacaccg
     4201 cccagacgag gctcaaggtg aggagcatgg cgagatacag gtcgccgcga gtcaatgtct
     4261 cccggagttc ataaccggcg atgacctcga cggagatgcg gacgagcagc acgacgcccg
     4321 acatgagcac caggggcgtc acgaggcgcc gctcacccga agtcaattga tcccgatgac
     4381 gcgagatgga ccacgccgcc gcgccaagcg cacccgccgc cgccaatccc acgagaaacg
     4441 cgccgttgaa ccacggacga taggtcgaca gatcgcggaa tgccgatgcg gtgtcccaca
     4501 ggagcgaatg tccgacagcc agcagcagca caccgtaagc cagcaggcgc tcgccgagtc
     4561 ccgcccgccc gaatccacca tgcatgagga tgagcgccag cagcgaccaa cccaacgtga
     4621 ccgctgcgag gtcgaactgg atcggtacgg ccagggccgc caggcccagc cccgtgagcc
     4681 gcgtcatctt cacggcccct tcgtccggac cgagccgcgc cccgtacgca cgcgccagca
     4741 tcacatagtc gagcgccagg atcgccgtgg caacgccccg caatggatga aagtccggcc
     4801 ccagcagatc gtaaagagcc gcctcataca ggaacccgtt ggccacgagc accagggccc
     4861 gcgccacgcc ccacccgcgc gccgccggca tggccgacag ggg
//
LOCUS       DASVOD010000379         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_965456, whole genome shotgun sequence.
ACCESSION   DASVOD010000379 DASVOD010000000
VERSION     DASVOD010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965456"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2008
                     /locus_tag="VF720_08720"
     CDS             <1..2008
                     /locus_tag="VF720_08720"
                     /inference="COORDINATES: protein motif:HMM:NF039734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HEX7879479.1"
                     /translation="VGHGEGGEFVIGNDTEDDDILSSSDAASSWGNNDMEWMGSVACQ
                     WLESSARAGWHDAMDGLHLICGSETNSRASPLYSYGGKWASKMKDSGAFDSPMTVKCG
                     WFDAYEAENPSGNVAVVIGETSTQGSDYLWGEGSVSSDPVHDGSYSYWRYTASGLRPF
                     TASKSDPSLSRSVEAITDAVSYRPEGGLPITVDRSLLEAPAIQLPYYMVMPANVDTPF
                     VRQIAQAICESEGVLCGGDIGPGDDGEINLIDGMYELRVCGDAGSYTYDAAGRYLSWR
                     TQPPNLPSPSVAQQLASQILAQWSGIGADAVFKGVRYVRQGMASHPNGEEIDDPQQSF
                     PVSIHALYQRHLGAQQIPVEGGGGHLSVAFGHNGQLDRVFVPGWHPVMMGGPVSILPV
                     QDVIQDIAARGSAATVDGITPPAQSATINGWYLAYYEPSCGVPLQRLRPIYVFNATVT
                     TIGGNESMTEIYAWADARSPVPDITSPAGPIAVPFGSQVCVTGQSMGGVPPVTLRWKD
                     DLGNQLGAGPSICFPAIMPPVGNHSEDDSVRTVALIARDALGSIATDILRIRILPGVS
                     GVGSVAPAGFGLLQNSPNPFRGLTQITLSVPELQPNESTTLRIYDVAGRLVNTLVNGR
                     LSPGDHSLTWDARDAGGNRVTPGIYFYEFRAGATTASRKLLVLE"
     gene            complement(2160..2258)
                     /locus_tag="VF720_08725"
     CDS             complement(2160..2258)
                     /locus_tag="VF720_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879480.1"
                     /translation="MITPVPGGVGPLTVAALCRNTVAAAVGEGSPL"
BASE COUNT          396 a          755 c          725 g          382 t
ORIGIN      
        1 cgtcggccac ggcgagggcg gggaattcgt catcggcaac gacaccgagg atgatgacat
       61 cctgagctcg agcgacgctg ccagttcctg gggcaataac gacatggagt ggatgggttc
      121 cgtcgcatgc caatggctcg agagcagtgc gcgggccggc tggcacgatg ccatggacgg
      181 acttcacctc atctgcggct ccgagacgaa ttcccgcgcg tcccccctct attcgtacgg
      241 cggcaagtgg gccagcaaga tgaaagacag cggggccttc gacagcccga tgaccgtcaa
      301 gtgcgggtgg ttcgatgcct atgaagcgga gaatccctcg gggaacgtgg ccgtggttat
      361 cggggagacc tcgacacagg gtagcgatta cctctggggc gaagggtccg tctccagcga
      421 tcccgtccat gacgggtctt acagctactg gcgatacacg gccagcggac ttcggccatt
      481 cacggcaagc aaatcggatc cgtcgctgag tcgctcggtc gaggccatca ccgacgcggt
      541 gagctatcgg ccggaaggag ggctccccat cacggtcgat cgcagcctgc tcgaggcgcc
      601 ggcgatccag ctcccctact acatggtcat gcctgccaac gtcgatacgc ccttcgtccg
      661 ccagattgcg caggcgatct gcgagtcgga aggggtcctg tgcggcggcg acatcgggcc
      721 gggcgacgac ggggagatca acctcatcga cggcatgtac gaattgcggg tgtgcggtga
      781 cgcgggttcc tacacgtacg acgccgccgg ccgatacctc tcatggcgga cgcagcctcc
      841 gaacctcccg agcccgtcag tggctcagca actcgcgagc cagatccttg cgcagtggtc
      901 cgggatcggg gccgatgccg tcttcaaggg cgtgcgctac gtgcgacagg ggatggcgag
      961 ccatcccaac ggcgaggaaa tcgacgaccc gcagcagtcg ttcccggtga gcatccatgc
     1021 cctgtaccag cggcatctcg gggcgcagca gatcccggtc gaagggggcg gcggccacct
     1081 gagcgtggcc ttcgggcaca atggccagct ggatcgcgtg ttcgtgcccg gatggcaccc
     1141 tgtcatgatg ggaggcccgg tctcgatcct tccggtgcag gatgtcatcc aggacatcgc
     1201 ggcccggggc agcgcggcca cggtcgacgg catcacgccc cccgcgcagt cggcgacgat
     1261 caacggctgg tacctggcct actacgagcc atcctgcggc gtgccgctcc agcgcctccg
     1321 tccgatctac gtcttcaacg caacggtcac taccattggc ggaaacgagt ccatgaccga
     1381 aatctatgcg tgggccgatg cgcgcagccc ggtcccggac atcacgtctc cggcgggccc
     1441 cattgccgtc ccgttcggtt cccaggtctg cgtcaccggg cagtccatgg gaggggtccc
     1501 gccggtcact cttcgttgga aggacgacct cggcaaccag ctaggcgccg gtccttccat
     1561 ctgcttcccg gcgatcatgc cgccggttgg caatcactcg gaggatgaca gcgtccggac
     1621 cgtcgcactg atcgcgcgcg acgccctcgg cagcatcgca accgacatcc tgagaatccg
     1681 cattctgccc ggagtttccg gagtcggatc cgtcgcgccc gccggcttcg ggctcctgca
     1741 gaattcaccc aatccgttcc ggggtttgac gcagatcacc ctcagcgtac cggagctcca
     1801 gccgaacgaa tcgacgacgc tccgcatcta tgatgttgcc ggccggctcg tgaacacact
     1861 ggtgaatgga cgtctctcgc ccggcgatca ctccctgacg tgggatgcgc gcgacgccgg
     1921 cggaaaccgg gtgactcccg gcatctactt ctacgagttc cgggccggag ccacgacggc
     1981 gagccgcaag ctgctcgtcc ttgagtaacg agcaaagcaa gtcacgcagc tgcgcgttga
     2041 cgaacggaag acgaaggggg taacgggccc gtcccgttac cccctttcct ttggttcttc
     2101 gcgacttttc gggaagggcg caggcatctt cccggtgtga gggcggatcc agtcgggcat
     2161 cacaggggac tcccctcccc caccgctgcc gccaccgtgt tccgacacag cgccgccacc
     2221 gtgagcgggc ccacgccacc cggtactggc gtgatcag
//
LOCUS       DASVOD010000380         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_495743, whole genome shotgun sequence.
ACCESSION   DASVOD010000380 DASVOD010000000
VERSION     DASVOD010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495743"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VF720_08730"
     CDS             <1..993
                     /locus_tag="VF720_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7879481.1"
                     /translation="YGPLANGATQVIYEGAPDWPKRDRFWELIEKFGVTIFYTAPTAI
                     RAFMKWGTEWPAKRDLSSLRLLGSVGEPINPEAWVWYHQNIGGTRCPIVDTWWQTETG
                     QILITPLPGITATKPGSCTRPFPGIAADILDNAGQSIPVGGGYLALTRPWPAMMRGIW
                     GDNERYKQTDWSKWQDLYFPGDGAKRDADGYFWVLGRVDDVMNVAGHRIGTMEVESAL
                     VDHPAVAEAAVVGKAHDLKGTAIAAFVTLKEGREAGDSLRDDLKEHVVKKIGAIARPD
                     DILFSADLPKTRSGKIMRRLLRDIAEGKALGDTTTLADPTVVATLRAKYEENES"
     gene            993..1706
                     /locus_tag="VF720_08735"
     CDS             993..1706
                     /locus_tag="VF720_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX7879482.1"
                     /translation="MPEIAGARSFDVRAPWKLVLPEGAAPAGGWPLVACLHGWGQSAD
                     YFARLCRPLLFDDAAFLFPQGPWSFEMRKEGKIRIGHAWYLYDGGDEPFRSTLGRAEE
                     HLLGVIDDVASAHPIDRARVALFGFSQGAYTGYFITLRNTDRFVAMAAMGGGRLKPEF
                     VEDELVGAPRIPYLLLHGLDDVSVPLERTSLMKGELERWGFPVELKEFPAAHEMTSDM
                     LDAVREYFRHTLASVSDST"
     gene            1870..>2125
                     /locus_tag="VF720_08740"
     CDS             1870..>2125
                     /locus_tag="VF720_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015161451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX7879483.1"
                     /translation="MDQGEGSSPFPVVGIGASAGGLEAFGKLLSGLSTETGMAFVLIQ
                     HLDPHHESRLVDLLGKMSPLPVVEATNGMSVRRNQVYVIPP"
BASE COUNT          374 a          654 c          733 g          364 t
ORIGIN      
        1 tacgggccgc tcgcgaacgg ggccacccag gtcatctacg aaggggcgcc cgactggccg
       61 aagcgggatc ggttctggga gctgatcgag aagttcggcg tcaccatctt ctacacggcg
      121 ccaacggcca tccgggcttt catgaaatgg ggcaccgagt ggccggccaa gcgcgatctg
      181 tcgtccttgc gcctgctcgg atccgtgggt gagccgatca atcccgaggc gtgggtctgg
      241 taccaccaga acatcggcgg cacgcgctgc ccgatcgtgg atacctggtg gcagaccgag
      301 acggggcaga tcctcatcac gccgctgcct gggatcaccg ccaccaaacc cggctcgtgc
      361 acgcggccgt tcccggggat cgcggccgac atcctggata acgcggggca gtcgattccg
      421 gtcggtggcg ggtacctggc gctcacgcgc ccctggccgg ccatgatgcg cggcatctgg
      481 ggcgacaacg aacgatacaa gcagaccgac tggtccaagt ggcaggacct ctacttcccc
      541 ggcgatggtg ccaagcgcga tgccgacggc tacttctggg tgctcggccg cgtggacgac
      601 gtgatgaacg tggcggggca tcgcatcggc acgatggagg tggagtcggc gctggtggat
      661 cacccggcgg tggccgaagc ggcggtcgtg gggaaagcgc acgacctgaa ggggacggcc
      721 atcgcggcgt tcgtgacgct caaggaaggg cgcgaggccg gggattcgct gcgcgacgac
      781 ttgaaggagc atgtcgtgaa gaagatcggc gccatcgcgc gacccgacga catcctcttt
      841 tcggccgacc tgccaaagac ccgctcgggc aagatcatgc gacgcctcct gcgcgacatc
      901 gccgaaggga aggccctggg cgacacgacc acgctggccg atcccaccgt cgtggccacg
      961 ctgcgggcca aatacgagga aaacgagagc tgatgccgga gatcgcgggg gcgcgctcgt
     1021 tcgatgtccg cgcaccctgg aagctggtgc ttcccgaggg tgcggcgccg gccggcggtt
     1081 ggccgctcgt ggcgtgcctg cacggctggg ggcagagcgc cgattacttt gcgcggctct
     1141 gtcgtccgtt attgtttgac gatgccgcgt tccttttccc gcagggcccc tggtcgttcg
     1201 agatgcggaa ggaaggaaag atccgcatcg ggcatgcctg gtacctgtac gacggcgggg
     1261 acgaaccgtt ccgctccacg ctggggcggg ccgaggaaca cctgctgggc gtgatcgatg
     1321 acgtggcttc ggcgcatccg atcgaccggg cgcgggtggc attgttcggc ttctcgcagg
     1381 gggcgtacac cggctacttc atcacgctgc gcaacaccga ccgctttgtg gcgatggcgg
     1441 cgatgggggg cgggcggctg aagccggagt tcgtggagga cgaattggtt ggtgcgccac
     1501 gcattccgta cctcctgctt catggactcg atgatgtgtc cgttccattg gaacggacaa
     1561 gcctcatgaa gggcgagctg gaacggtggg ggttcccggt ggaattgaag gagttcccgg
     1621 cagcgcacga aatgacatcc gacatgctgg acgcggtccg ggagtacttt cgacacactt
     1681 tggcttctgt cagcgactcc acctagacct agcctcctga tcgggaaatc ccccagaagg
     1741 gccgcgcgcg acgtctggta acccgtattc gtatgattct agaatcgagt aacgggagga
     1801 ctcgtggcaa agcagacccc gctcgaatcc gagaaggaca tcgttccgga gcagcactca
     1861 aagggctccg tggaccaggg agaagggagc agccccttcc ctgtcgtcgg catcggcgcc
     1921 tccgccggtg ggctggaggc cttcggcaag ctcctctccg gcctgtcgac cgaaacgggc
     1981 atggccttcg tgctgatcca gcatctcgat ccccaccatg agagtcgcct cgttgatctg
     2041 ctcggcaaga tgtcgccgct ccccgtggtc gaagcgacaa acggcatgtc ggtacggcgg
     2101 aaccaggtgt acgtcattcc gccga
//
LOCUS       DASVOD010000381         3016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_130946, whole genome shotgun sequence.
ACCESSION   DASVOD010000381 DASVOD010000000
VERSION     DASVOD010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130946"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VF720_08745"
     CDS             complement(<1..583)
                     /locus_tag="VF720_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7879484.1"
                     /translation="MSIFIDRKTKVLVQGITGRDGAFHTKKMLEYGTQIVGGVTPGKG
                     GETMHDVPVFNTVADAVKATGADATVIYVPAAFCADAIYEAADAGLKFIVAITEGVPA
                     LDMVECVPYVKSKGARLLGPNCPGLITPGQCNIGIMPGRICTPGNVGLVSRSGTLTYE
                     AVDALTRAGLGQSTCVGIGGDPVNGTSFLDCLAA"
     gene            complement(580..1719)
                     /gene="sucC"
                     /locus_tag="VF720_08750"
     CDS             complement(580..1719)
                     /gene="sucC"
                     /locus_tag="VF720_08750"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX7879485.1"
                     /translation="MNLHEFQGKEIFGRFGIPVPEGKVARTAAEAGALAKSLGGAVVV
                     KAQVHSGGRGKAGGVKLAKDAAEAEARAKAILGMDIKGHRVDQVLITPATDIAREIYL
                     GIVLDRVARKPVIMASAAGGIDIEEVAATSPEKIVKFHLDGKGTFRPWMARWVAFQIE
                     KDPKAASQVAAILEKLVKVYWECDCSLAEINPLVVTPEGKVVAIDAKVVLDDNGLVRH
                     KEFEALRDGESDAERLAREKGLSFIPLEGTIGCVVNGAGLAMATMDLVKHFGAEPANF
                     LDIGGSSNPEKVVAAMTIITADPNVKAILFNIFGGITRCDDVARGIVSALETKPVSLP
                     IVVRLTGTNEEEGRAILANARGITPAATMDEAAQKVIAMANGGAA"
     gene            complement(1739..>3016)
                     /locus_tag="VF720_08755"
     CDS             complement(1739..>3016)
                     /locus_tag="VF720_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015859687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX7879486.1"
                     /translation="NFNTVYRNDAGVNVAAFTATQIPNIENRVYPAVLAGPLYPDGIP
                     ILPESDLEAIMTRHAPCDVVFAYSDVSHEYVMHMASRVLALGGNFVLLGPSATAIPAR
                     IPVVAVGAVRTGAGKSQTSRKVVEVLRERGLKVVVVRHPMPYGELARQGVQRFATMDD
                     LVKADCTIEEREEYEPHLERGSIVFAGVDYGAILAEVEKEAQVLLWDGGNNDFPFYRP
                     DILITVADPHRPGHEVRYHPGETNFRMADVIVINKETTADYAGIRAVEESIAALNPKA
                     QVIHAASPIFVDNPGLIRGKRALVVEDGPTLTHGEMTYGAGVIAARQFGASELVDPRP
                     WVTGEMAGTFRSYPKIGTLLPAMGYSPAQIKDMERTINATDCDVVVIATPIDLGRLMT
                     LDKPAVRVRYELEEIGQPKLGDILNDFCARKGL"
BASE COUNT          448 a          998 c         1014 g          556 t
ORIGIN      
        1 aggcggccag gcaatcgagg aagctcgtgc cgttgaccgg gtcgccaccg atgccgacgc
       61 aggtggactg gccgaggccg gcccgagtaa gcgcgtcaac cgcttcgtag gtgagcgtgc
      121 cggagcggga gacgagcccc acgtttccgg gcgtgcagat gcgtcccggc atgatgccga
      181 tgttgcactg cccgggggtg atgaggccgg ggcagttggg gccgaggagg cgcgcgccct
      241 tcgacttcac gtacggaacg cactcgacca tgtcgagcgc gggaacgccc tcggtgatgg
      301 ccacgatgaa cttgaggccg gcatcggccg cctcgtagat ggcgtcggcg cagaaggcgg
      361 ccggcacgta gatgaccgtg gcgtcggcgc cggtggcctt caccgcgtcg gccaccgtgt
      421 tgaagaccgg cacgtcgtgc atcgtctcgc cacccttgcc gggcgtgacg ccgccgacga
      481 tctgcgttcc gtactcgagc attttcttcg tatggaaggc accgtcgcgg ccggtgattc
      541 cctggaccag caccttcgtc ttgcggtcga tgaagatgct catgccgcac ctccgttcgc
      601 catcgcgatg actttctggg ccgcctcgtc catggtggcc gccggcgtga ttcccctggc
      661 gttggccagg atggcgcgcc cttcctcttc gttggtgccg gtcaggcgca ccacgatcgg
      721 gagcgacacg ggcttcgtct cgagggcgct cacgatgccc cgcgccacgt cgtcacagcg
      781 ggtgatgccg ccgaaaatgt tgaaaaggat ggccttcacg ttggggtcgg cggtgatgat
      841 cgtcatggcc gccaccacct tctcggggtt ggatgagcca ccgatgtcga ggaagttggc
      901 cggctcggcg ccgaaatgct tgaccaggtc catggtggcc atggccaggc cggcgccgtt
      961 gaccacgcag ccgatcgtcc cctcgagcgg gatgaacgac agccccttct cgcgggccag
     1021 gcgctcggca tcgctctcgc cgtcgcgcag cgcttcgaac tccttgtggc gcacgagccc
     1081 gttgtcgtcg aggacgacct tggcgtcgat ggccacgacc ttcccctcgg gcgtgacgac
     1141 gagcgggttg atttccgcga gtgagcaatc gcactcccag tacaccttca cgagcttctc
     1201 gaggatggcc gccacctggg aggccgcctt cggatccttc tcgatctgga aggccaccca
     1261 ccgggccatc cacgggcgga acgtcccctt cccgtcgagg tggaacttga cgatcttctc
     1321 aggggaggtg gccgccacct cctcgatgtc gatgccgccg gcggccgagg ccatgatcac
     1381 gggctttctg gccacgcggt cgagcacgat gccgagatag atctcgcggg cgatgtcggt
     1441 ggccggcgtg atgagcacct ggtccacccg atgccccttg atgtccatgc cgaggatggc
     1501 cttggccctg gcctcggcct cggcggcatc tttcgccagc ttcacgccgc cggccttgcc
     1561 gcgcccgccc gagtgcacct gcgccttgac caccacggcg ccgcccagcg acttcgccaa
     1621 ggcgccggct tcggccgcgg tacgggccac ttttccttcg ggcaccggga tgccgaagcg
     1681 gccgaagatc tccttcccct ggaattcatg gagattcacg ttggatcgat cctttccgtc
     1741 aaagcccttt tcgggcgcag aagtcgttca ggatgtcgcc cagcttcggc tggccgatct
     1801 cttcgagttc gtagcgtacc cgcacggcgg gcttgtcgag cgtcatgagc cggccgaggt
     1861 cgatcggcgt ggcgatcacc accacgtcgc agtccgtggc gttgatggtg cgctccatgt
     1921 ccttgatctg tgccgggctg tatcccatgg ccggcagcag cgtgccgatt ttcgggtagc
     1981 tcctgaacgt gccggccatc tcaccggtga cccaggggcg cggatcgacg agctccgacg
     2041 cgccgaactg tcgcgccgcg atgacgccgg cgccgtaggt catctcaccg tgcgtgagcg
     2101 tgggaccgtc ctcgaccacc agggcgcgct ttccccggat gagcccgggg ttgtcgacga
     2161 agatggggga tgcggcgtgt atgacctgcg ccttcgggtt gagcgccgcg atcgattcct
     2221 caacggcccg gatgccggcg tagtcggccg tggtttcctt gttgatgacg atgacgtcgg
     2281 ccatccggaa attcgtttcg cccgggtgat agcggacttc gtgacccggg cggtgcggat
     2341 cggccaccgt gatgaggatg tcgggacggt agaagggaaa atcgttgttc ccgccgtccc
     2401 acaggagcac ctgggcctcc ttctccacct cggccaggat ggcgccgtag tcgacgcctg
     2461 cgaagacgat gctcccccgc tcgaggtgcg gttcgtattc ctcgcgttcc tcgatggtgc
     2521 agtcggcctt gaccaggtcg tccatcgtgg cgaagcgctg caccccctgg cgggccaact
     2581 cgccgtaggg catcggatgc cgcaccacca ccaccttgag gccgcgctcg cgcagcactt
     2641 ccaccacctt gcggctggtc tggctcttgc cggcgccggt gcgtacggcc cccaccgcca
     2701 cgaccgggat cctggccggg atggccgtgg ccgagggccc gagaaggacg aagttgcctc
     2761 cgagcgcgag tacccgcgat gccatgtgca tcacgtactc gtgggagacg tcgctgtagg
     2821 cgaagacaac atcgcacggc gcgtgccggg tcatgatcgc ctcgaggtcg gattcgggaa
     2881 ggatgggaat cccatccggg tagaggggac ccgccagcac ggccggatag acgcggttct
     2941 cgatgttcgg gatctgggtg gccgtgaagg ccgccacgtt caccccggca tcgttccggt
     3001 agaccgtgtt gaagtt
//
LOCUS       DASVOD010000382         5311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_755676, whole genome shotgun sequence.
ACCESSION   DASVOD010000382 DASVOD010000000
VERSION     DASVOD010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755676"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF720_08760"
     CDS             <1..269
                     /locus_tag="VF720_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX7879487.1"
                     /translation="RTLARVDREVELDVSGPVTTRSVLDTLEARYPVLKGAIRDHGTL
                     KRRDFLRFFACQEDWTHEPVDAPLPDAIIAGREPFLIVGAIAGG"
     gene            256..1188
                     /locus_tag="VF720_08765"
     CDS             256..1188
                     /locus_tag="VF720_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012233207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX7879488.1"
                     /translation="MPGDSGAPTRFAPSTTGPAHPGTLLSALLCWLDARSRGAAVHLR
                     LEDLDPERCRPEFADAMRRDLEWLGLDWDGVITQSDRRAAHEAALDELAARGLLYPCD
                     CSRARLKSIGRAAPDGGFAYDNQCRSRELPPGGWRAVSAPLRLRLPEDCVSFVDELGL
                     VIEQRPAHDMGDPVVRRRDGAIAYHLASVVDDAAGGFTRLIRGRDLAPSAPTQRLIYG
                     RLGLPIPTYRHHFLLLELRGDKLAKLHGSVGVEALRARHSSPELVGMLAAWAGIHAEG
                     AACRPVDLVASFDWARVRRYDLAVAWDGSRLSAH"
     gene            complement(1185..2291)
                     /locus_tag="VF720_08770"
     CDS             complement(1185..2291)
                     /locus_tag="VF720_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX7879489.1"
                     /translation="MPKRPPHDINMEVAVHLHDMGLAHAQQHGRIAYRRAARVMMALE
                     TPLDVLLASHSPRDIPGIGPASERIILEHLRTGESPTVARAVEKSAQRSEVLAARKIR
                     TNFLSRAAATLILARPMPGVIQVSDYKGDLQMHTEWSDGGETIGDMIDAATLRGYRFI
                     GVSDHSYGLKIARGMTMETAARQHEEIDRLNAKYAPDFRVFKGIEANIPLEGGVDMTN
                     AELKHFQIVLAAPHSKLRKTEDQTDRMLATVRHPRVGILAHPRGRMFTRLGVQADWDK
                     VFAEAAARKVAIEIDGDPWRQDLDWVLARRALDAGCLFALDSDAHNGAQLGYAEFAVA
                     HARLAGIPPERVINTWTATDLLAWARRRRPRASR"
     gene            2512..4632
                     /locus_tag="VF720_08775"
     CDS             2512..4632
                     /locus_tag="VF720_08775"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX7879490.1"
                     /translation="MDGDIGDSAWASVPVIDTWFETNVGDNVEPQVKNRARLAYDDHF
                     LYAAFEFGDPNPGGIRAPLGDHDAVGGSTDYAGLIIDSRNDAKTARMFLANPRGVQYD
                     AVTSDVSGEDSSPDFYWDSAGKITESGWTLELRIPFSSLRYSDTEAPTWGMLMYRNYP
                     RDRRYQFFSARLPRDVNCFICNSSKMTGLSSLPQGSHLVVAPFVTGTQESVPKDGLGT
                     ALESGDLDGEAGVDIKWSPASTVALDATVNPDFSQVESDVAQIAANERFALFFPEKRP
                     FFLEGVDLFSTPMQAVYTRTVTSPSAGLRATGRMGETSYTVLATHDEGDGTVIIPGAQ
                     GSEQVSQDFQSGVGIARVRRDLGQSFVSVLATSRLVEGGGYNHVFGPDFQWRPNPRDN
                     FTGQLLFSETETPDRQDLSEQWDGRKLSDVAGLLYWSHGTRHEDVFVQLQSLGEDFRA
                     DNGFIPQVGYSEGYFEAGYTIRPTDFFLNRIRPFTIHYVDIDTDGEVLSQRSSFGTGM
                     DGRWNSFLRYELNWDAMRVGDELLRRFRPRIQATAVPGRVVNSLAIDTYFGEEIDFAN
                     AREGTGVTLAGSFILRPSHHLELRGNLSYRWLNVDEPTLSGRVFTAEVERLRATWAFN
                     SRSFLRLIGQYTETTRNPELYDNPVSKKSANFGGSALFAYKINWQTVLYAGYGDQHDY
                     LGETDSLEPSGQQFFTKVSYAWQH"
     gene            complement(4678..>5311)
                     /locus_tag="VF720_08780"
     CDS             complement(4678..>5311)
                     /locus_tag="VF720_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX7879491.1"
                     /translation="GGRLARLNVGVADALPKLVVRRLLDPLHRLDEPVRLVARDDKPD
                     RLLADLARHELDVVLTDAPVGPGIAVKAFNHQLGECPVAFYAAAKLATRLRRNFPRSL
                     DGAPVILPTENSTLRRSIDSWLERVGIRPLIVAEFEDSALLTSFGEDGRAAFPFPTVV
                     GAALRRQVAAVSCGTLPGIVERFYAITVERRIRHPAVAAICTSARTRLFG"
BASE COUNT          823 a         1727 c         1869 g          892 t
ORIGIN      
        1 tgcgcacgct ggcccgcgtc gatcgcgagg tggaactcga cgtgtcggga ccggtcacca
       61 cgcgatcggt cctcgacacg ctcgaggccc gctacccggt gctgaaggga gccatccgcg
      121 accacggcac cctgaagcgc cgcgattttc tccgcttctt cgcctgccag gaagactgga
      181 cgcacgaacc ggtggatgcg ccgttgcccg acgcaatcat cgcgggcagg gagccgttcc
      241 tgatcgtggg ggccattgcc gggggatagc ggggcgccca cgcgcttcgc gccctccacc
      301 accggtccgg cacaccccgg aacgctgttg tcggcgcttc tctgctggct ggatgcgcgc
      361 tcgcggggtg cggccgtgca cctgcgcctc gaggacctcg accccgagcg gtgtcggccg
      421 gagtttgccg acgccatgcg gcgcgatctc gagtggctcg gactcgactg ggacggggtg
      481 atcacgcaga gcgatcgacg ggcagcgcat gaggcggcgc tggatgaact cgcggctcgg
      541 ggattgctgt acccgtgcga ctgcagccgc gcccggctca agtcgatcgg ccgcgcggcc
      601 ccggatggcg ggtttgcgta cgacaaccag tgtcgttcgc gtgagttgcc gccgggtgga
      661 tggcgcgccg tgtcggcgcc gcttcgcctg cggcttccgg aagattgcgt gtcgttcgtg
      721 gatgagctcg ggctcgtcat cgagcagagg cccgcgcacg acatgggtga tccggtcgtc
      781 cgccgccggg acggcgcgat cgcctatcac ctggcatcgg tggtggacga cgcggcgggg
      841 ggattcacgc ggttgattcg cgggcgggat ctcgcgccga gtgcgccgac gcagcggttg
      901 atctatggac gactcgggtt gccgattccg acctatcgac atcacttcct gctgctggaa
      961 cttcggggcg acaagctggc caagctgcat gggtcggtgg gcgtggaggc gcttcgcgcc
     1021 aggcattcgt caccggagct ggtggggatg ctggcggcgt gggcgggaat ccatgcggag
     1081 ggcgcggcct gccggccggt tgatctcgtg gcctcgttcg actgggcgcg cgtacggcgg
     1141 tacgacctcg ccgtggcgtg ggatgggagc cggctgagcg cgcactagcg tgaggcgcgg
     1201 ggtcgccggc gacgggccca ggccaggagg tcggtggcgg tccaggtgtt gatgacgcgc
     1261 tcgggaggga tgccggccag tcgagcgtgg gcgacggcga actccgcgta gccgagctgc
     1321 gcgccgttgt gggcgtcgct gtcgagggca aacaggcagc cggcgtcgag ggcgcggcgg
     1381 gccagcaccc agtcgaggtc ctgccgccag gggtcgccgt cgatctcgat ggccaccttg
     1441 cgcgcggccg cttcggcgaa gaccttgtcc cagtcggcct gcacgccgag tcgcgtgaac
     1501 atgcggccgc gtgggtgcgc caggatgccg acacggggat gacgaacggt ggccagcatc
     1561 cgatcggtct ggtcctcggt cttgcggagc ttcgagtgcg gcgcggccag cacgatctgg
     1621 aaatgcttga gctccgcgtt cgtcatgtcc acgcctcctt ccagcgggat gttggcctcg
     1681 atgcccttga agacgcggaa gtccggcgca tacttcgcat tgaggcggtc gatttcctcg
     1741 tgctgtcggg ccgccgtctc catggtcatc ccccgcgcga ttttcaggcc atacgaatgg
     1801 tcgctgacac cgatgaaccg atacccgcgc aacgtggcgg catcgatcat gtcgccgatc
     1861 gtttcgcctc cgtcgctcca ctcggtgtgc atctggaggt cgcccttgta gtcggagacc
     1921 tggatcaccc cgggcatggg gcgcgccagg atcagggtcg cggcggcccg gctcaggaaa
     1981 ttcgtgcgga tcttccgggc ggccagcact tcggatcgct gggcgctctt ttccaccgcc
     2041 ctggccaccg tggggctctc gccggtacgc aggtgctcca ggatgatccg ctcgctggcc
     2101 gggccgatcc ccgggatgtc gcggggggaa tggctggcca ggaggacatc gagcggggtt
     2161 tcgagcgcca tcatgacccg ggcggcccgg cggtaggcga tccggccgtg ctgctgggca
     2221 tgggcgaggc ccatgtcgtg aaggtggacg gccacctcca tgttgatgtc gtgcgggggg
     2281 cgctttggca tggggtttat cgtagtccga tgtccggaac cggacgctcg ggaaaaacct
     2341 gatgaatggt tgaacgattc agtcacccgg ggcgtccaac gcgccatgac cgcacgcacc
     2401 cccctcgtgg cctcgctagc cctgctgtgt ctttttgccc ctgtcgttca ggccgcagat
     2461 ccggtcccgg gcccgcctct cggaatcgtc aagctgaccg gggaaatctc cgtggacggc
     2521 gacatcggtg attccgcctg ggcgtcggtg ccggtgatcg atacctggtt cgagaccaac
     2581 gtcggcgaca atgtcgagcc ccaggtgaag aaccgggccc gattggccta cgacgaccac
     2641 tttctgtatg ccgcattcga gttcggagac ccgaatccgg gcggcatccg cgccccgctg
     2701 ggcgatcacg acgcggtggg aggctcgacc gactacgccg ggctgatcat cgacagccgg
     2761 aatgacgcga agaccgcccg catgttcctg gccaaccccc gcggcgtgca gtacgacgcg
     2821 gtgaccagcg acgtgtcggg cgaggattca tctcccgatt tctactggga ttcggcgggg
     2881 aagatcaccg aatcggggtg gacgctcgag cttcgcattc ccttttcgtc gctgcgctat
     2941 tcggataccg aggcgccgac ctggggcatg ctgatgtatc gcaactaccc gcgcgatcgg
     3001 cgctaccagt tcttttccgc ccggctcccg cgcgacgtca attgcttcat ctgcaactcc
     3061 agcaagatga cgggcctctc gagcctgccc caggggtcgc atctcgtggt ggcgccgttc
     3121 gtcaccggca cccaggaatc cgtgccgaag gatggtctcg gcacggcgct cgaaagcggc
     3181 gacctggacg gcgaagccgg cgtggacatc aagtggagcc cggcctccac ggtggcgctc
     3241 gacgccaccg tgaaccccga cttctcgcag gtggaatccg acgtggcgca gatcgcggcc
     3301 aacgagcgct tcgccctctt cttcccggaa aagcgtccct tcttcctcga aggggtggac
     3361 ctcttctcga cgccgatgca ggccgtttac acccgcaccg tcacgtcgcc gagcgcgggg
     3421 ctcagggcca ccgggcggat gggggagacg tcgtacacgg tgctggccac tcatgacgag
     3481 ggggacggga cggtcatcat cccgggcgcg caggggtcgg aacaggtgtc gcaggacttc
     3541 cagtcaggcg tcggaatcgc gcgcgtgcgc cgcgacctcg ggcagtcgtt cgtgagcgtc
     3601 ctggccacca gccgcctggt ggaaggagga ggctacaacc atgtcttcgg ccccgacttc
     3661 cagtggcggc cgaacccgcg cgacaacttc accggccagc ttctcttcag cgaaaccgaa
     3721 acgcccgatc gccaggacct gtcggagcag tgggacggcc gcaagctctc cgacgtggcg
     3781 gggctcctct actggtcgca cggcacgcgg catgaggacg tgttcgtgca gctgcagtcg
     3841 ctcggggagg acttccgggc cgacaacggc ttcatcccgc aggtgggtta ctcggaagga
     3901 tatttcgagg cggggtacac catccggccc accgacttct tcctgaaccg gatccggccg
     3961 ttcaccattc actacgtgga tatcgacacc gacggcgagg tgctgagcca gcgctcatcc
     4021 ttcggcaccg gcatggacgg ccggtggaac agcttcctgc gatacgagct gaactgggat
     4081 gccatgcgcg tcggtgatga actgctgcgc cgcttccggc cgcgcatcca ggccaccgcg
     4141 gtgccgggac gggtcgtgaa cagcctggcc atcgacacgt acttcggcga ggagatcgac
     4201 ttcgccaacg cgcgggaagg aacgggcgtg acactggccg gatcgttcat cctgcggccg
     4261 tcgcatcatc tcgaactgcg cggcaacctg agctatcgat ggctgaacgt ggatgagccg
     4321 acgctgtcgg ggcgcgtgtt caccgcggag gtggagcggc tgcgggccac ctgggccttc
     4381 aactcccgct cgttcctgag attgatcggc cagtacaccg agacgacgcg caatcccgag
     4441 ctctacgaca acccggtgtc gaagaagagc gccaatttcg gcggctcggc cctctttgcc
     4501 tacaagatca actggcagac ggtgctctac gccggctacg gcgaccagca cgattacctg
     4561 ggggaaaccg attcgctcga gccgagcggg cagcagttct tcaccaaggt atcgtacgct
     4621 tggcagcact gaggcgggcg cgtcggccag ggctggcggt tcatcggccg gacgccctca
     4681 accgaacagc ctggtgcgcg cgctcgtgca gatcgcggcc acggcggggt gccggatgcg
     4741 ccgctccacg gtgatggcgt agaaacgctc gacgatcccc gggagggtgc cgcatgacac
     4801 cgcggcaacc tgcctccgca aggccgcacc cacgacggtg gggaagggga acgcggcccg
     4861 cccatcctca ccgaacgacg tgagaagggc gctgtcctcg aactccgcga cgatcagggg
     4921 gcggattccc acgcgttcca gccaggaatc gatcgagcgc cgcagtgtgg aattctcggt
     4981 ggggaggatg accggcgcgc cgtcgagcga gcggggaaag ttgcggcgca ggcgcgtggc
     5041 cagcttcgcg gcggcataga aggccaccgg acactcgcca agctggtggt tgaatgcctt
     5101 cacggcgatg ccggggccga ccggcgcgtc ggtgagcacg acatccagtt cgtgcctggc
     5161 caggtcggcc aggaggcggt cgggcttgtc gtcccgcgcc accaggcgca ccggctcgtc
     5221 gaggcggtgg agcgggtcga ggagtcgacg gaccaccagc tttggcagcg catcggccac
     5281 gcccacgttg aggcgcgcca ggcgcccgcc c
//
LOCUS       DASVOD010000383         6288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_444279, whole genome shotgun sequence.
ACCESSION   DASVOD010000383 DASVOD010000000
VERSION     DASVOD010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6288
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444279"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1530)
                     /locus_tag="VF720_08785"
     CDS             complement(235..1530)
                     /locus_tag="VF720_08785"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX7879492.1"
                     /translation="MTRSTTLPALAAAFLLLAGCGTEAPDQPIAATGDYQPIVLGSTV
                     DPSLPAPGNEAAAVDPDDPVLAAAPASFGLTPAAAGNPVVYWNEYVKTIGTAASLPPP
                     ALSRAYSLVMVGIYDALVAGNDSRRGGKLSANAAAAGAACEVLVYLFPGKAEQIQSDA
                     ALEAGISRGNGAALGGWNMGRAVGRQIVKFGKADGSSEVFAGTIPTGPGIWTGTNPVL
                     PLAGHWTCWVLDGGDEIAPPPPFAYGSPEDLLELQAVIDAAANRTPGQIEAVHKWGDR
                     SPPAIWNTSYINPRLLASGFDPVESARISAFANIAMTDAFINCWACKYHFWTARPSQR
                     IAGLVTVIPTPNFPSYTSGHSTISASVAEVLADVWPDEASYFRAEANEAAMSRLWGGI
                     HFPQDNSVGATIGREVGIRVVERMHEKAPIHAAPVLAMR"
     gene            1733..2995
                     /locus_tag="VF720_08790"
     CDS             1733..2995
                     /locus_tag="VF720_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879493.1"
                     /translation="MPRTLTCFRTAAALFPALLSMALAHPADAANCGNTSTGMIPIID
                     LGPATYQGFQGGLYPGGVNFRPSAHESAGVAIAQSVTRLNKSGDPDPQGKIVMASIGM
                     SNCYLEYGAFKNYADGYPGVNWGIQFVNLAQGGQVAETICNPNAEYWDYVDQQLWWND
                     VTAEQIQVVFFKNANRSPDGGFPGQAQTYGKQVGDIMHILHDTFPNLKQVFITSRTYG
                     GYASIPLNPEPYAYESGFGIKWLIEAQIAGEDSLNFDPAQGPVRCPWLSWGPYLWADG
                     ENPRSDGLTWPCSMFDSADGTHPNPAGQAVVAQIMTDWFLGDTASSPWFYTAVSGVPA
                     PVDITRPFSSPLPARARTTLTWNEATPPSEVSIFDVTGRRVRQLVGATDRWDLSDDAG
                     CPVPDGIYWARPSGAGPERTARIVVQRN"
     gene            3079..3912
                     /locus_tag="VF720_08795"
     CDS             3079..3912
                     /locus_tag="VF720_08795"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEX7879494.1"
                     /translation="MDILLLLKALVLGVVEGFTEFLPISSTGHLIVAASLLRFTPASA
                     AAFEIAIQTGAMLAVVWEYRARIGRTVAGLASRPADRKFALNLAVAFLPAMVFGLAFG
                     KLIKRHLFHPVPVAIAFAVGGVIILLVEGAIRRRGAPPRVGRVDDMSLLDALKVGLVQ
                     CLALIPGTSRSGATIIGGLVFGLSRPAATEFSFFLAVPTLMAAGAYSLYKERDFLTAA
                     DLPLFLVGTVVSFVSALVVIRWLIRYVAGHDFTAFAWYRIAFGAIILWTAWSGLVSWN
                     P"
     gene            3909..4439
                     /locus_tag="VF720_08800"
     CDS             3909..4439
                     /locus_tag="VF720_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX7879495.1"
                     /translation="MMSIDWHALFVPHGSLLEIVVRGTVMYLVLFILLRVLVRRHVGA
                     MGVPDLLVIVLIADAAQNAMAGEYKSITEGALLCAVIIGWSVLFDWLAYRFKPLRGIL
                     EADPLPVIKDGVMLPRNMRQELITRDELLSHLREQGIDDPADVALACMEPDGAISVIK
                     RDGKQDAHPPKRKPVQ"
     gene            complement(4452..5918)
                     /locus_tag="VF720_08805"
     CDS             complement(4452..5918)
                     /locus_tag="VF720_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX7879496.1"
                     /translation="MAGEIRHADGRVELADTSRVMASPLSNEDLAPVPIGRRNWTTYN
                     YAALWISMAHCIPTYMLASGLMAAGMNWLQALITVLLGNTIVLAPILLNSHPGTKYGI
                     PFPVFARAAYGTLGSNLPALMRALVACGWFGIQAWIGGEALHTLFSTLIPGWANLLGP
                     GFAGHSTTAWLSFLLFWGINIFIIYRGMDLLREVENWAAPFVLIMTAVLLGWAIMKAD
                     GLGPLLAQPGKFKTFGEFLPVFIPSLTAMIGFWATLSLNMPDFTRFGRSQKEQMIGQV
                     VALPTTMSVFAAMGILITSATVIIYGRAIWDPVKLVGEFKSPFVVIISMFTAVVATLA
                     VNIAANVASPANDFANAFPKAINFKTGGLITGLIGILMQPWKLLADPSGYIFTWLLGY
                     SGGLGSIAGVLIADYWLIRKKELKLEDLYLPDGAYRYSGGWNWKAVLATLFGCALAWG
                     GLVIPLMKPLYDYAWFVGFAGAGLLHWLLMRGDVSTRA"
BASE COUNT         1053 a         2038 c         2129 g         1068 t
ORIGIN      
        1 atcatcgacg cccaggggcg cgtcgtccga tcgtccctct tcatcccggg gggcgcgctg
       61 gaattcggct gggacgggcg gaccgatgcg ggcgcgcccg ccccgcccgg tgtctatttc
      121 gccctcctgc gggatgggtc cgcgcctcgc gtgggccgga tcgtgctggc gcgatagacg
      181 cttccggata accgcgccac cgatcaagac tccccaacga tggaacgccc cgatctatcg
      241 catcgccaat accggcgccg cgtggatcgg cgccttttcg tgcatccgtt ccaccacgcg
      301 aatcccgacc tcgcggccga tcgtggcccc cacggagttg tcctgcggga agtggatgcc
      361 gccccagagc cgcgacatgg cagcctcgtt ggcctcggcc cggaagtagg aggcctcatc
      421 cggccagacg tcggccagta cctcggccac ggaagcggag atggttgagt gccccgaggt
      481 atagctcggg aagttgggtg tcggtatcac ggtgacgagt ccggcaatcc gctggctcgg
      541 tcgcgccgtc cagaagtgat acttgcaggc ccagcaattg atgaaggcat ccgtcatcgc
      601 gatgttggcg aaggcgctga tcctggccga ctccacggga tcgaaaccgg acgccagcag
      661 gcgcgggttg atgtagctcg tgttccagat ggccggcggc gaacgatctc cccacttgtg
      721 cacggcctcg atctgcccag gcgtgcgatt ggcggcggca tcaatcacgg cctgcagttc
      781 gagcaggtcc tccggcgagc cataggcgaa cggaggagga ggagcgatct cgtcgccacc
      841 atcgagcacc cagcaggtcc agtgaccggc gagggggagt acggggttcg tacccgtcca
      901 gatccccggg ccggtgggaa tcgtcccggc aaagacctcc gaggaaccat cggccttgcc
      961 gaatttcacg atctgccttc ctacggcccg acccatgttc cagccgccga gcgcggcgcc
     1021 gtttccccgg gagattcccg cctcgagcgc ggcgtcggac tggatctgtt cggccttgcc
     1081 ggggaagagg tagacgagca cttcgcaggc ggcaccggcg gcggcggcat ttgcggagag
     1141 cttcccgcca cgacgactgt cgttgccggc cacgagtgcg tcgtagatcc ccaccatcac
     1201 cagtgagtag gcgcgcgaca gggcgggggg cgggagcgag gccgccgtgc cgatcgtctt
     1261 cacgtactcg ttccagtaga cgaccgggtt gccggccgcg gccggtgtca gcccgaacga
     1321 ggccggggcc gcggcaagga caggatcgtc cgggtctacg gccgcggcct cgtttccggg
     1381 tgccgggagg gatggatcga ccgtggagcc caggacgatc ggctggtaat cgcccgtggc
     1441 ggcgatcggc tggtcgggag cctcggtgcc gcaaccggcg aggaggagga aggcggcggc
     1501 cagggcagga agggtggtgc tgcgcgtcat cggtgtctcg ctttcgaggt cacggaagca
     1561 ggatgaacgc ggccagcgtg ccacgatgat aggtttcgaa tcggcagcgg caagggaaaa
     1621 tgttgtccgt ccggctgccc cgaccgggga tttccccatg cggcatcatc acttgccggg
     1681 cttcatgaga tagaagtgga ccggtgcccg cgcatcccgg ataattgtcc gaatgccccg
     1741 caccctgacc tgcttccgca cggccgccgc gctctttccg gccctcctga gcatggccct
     1801 cgcgcatcct gccgatgccg ccaactgcgg caacacctcc accgggatga ttccgatcat
     1861 cgacctcggg ccggctacct accagggatt ccaggggggg ctctatccgg gaggcgtgaa
     1921 tttccgtcct tcggcccatg agtcggccgg tgtcgccatc gcccaatccg tcacgcgcct
     1981 caacaagtcg ggcgatcccg atccgcaggg gaagatcgtc atggcctcga tcggcatgag
     2041 caactgctac ctggaatacg gcgccttcaa gaactacgcg gatggctacc cgggtgtgaa
     2101 ctggggcatc cagttcgtga acctggccca gggggggcag gtggccgaga cgatctgcaa
     2161 tccgaacgcc gagtactggg actacgtgga ccagcagctc tggtggaacg acgtcaccgc
     2221 cgagcagatc caggtcgtgt tcttcaagaa cgccaaccgg agccccgacg gcggctttcc
     2281 cggccaggcg caaacgtacg gcaagcaggt gggggacatc atgcacatcc tccacgacac
     2341 ctttcccaac ctgaagcagg tgttcatcac cagccgcacc tacggcggct acgcctccat
     2401 cccgctcaac ccggagccgt acgcctacga gagcggtttc ggcatcaagt ggctgatcga
     2461 ggcccagatc gccggcgaag acagcctgaa cttcgacccg gcacaggggc cggtgcgctg
     2521 cccatggctc tcgtgggggc cgtacctctg ggccgatggc gagaacccgc ggagcgacgg
     2581 cctcacctgg ccgtgcagca tgttcgactc ggccgacggc acgcatccca atccggccgg
     2641 ccaggccgtc gtggcgcaga tcatgaccga ctggtttctt ggcgataccg catcgagccc
     2701 ctggttctac accgcggttt cgggggtgcc cgcgccggtc gacatcacgc gccccttctc
     2761 atcgcccctg ccggcgcgcg cgcggaccac gctcacctgg aacgaggcca cgcccccctc
     2821 ggaggtgtcc atcttcgatg tgacggggcg acgtgtacgc cagctggtcg gcgcaaccga
     2881 tcgctgggat ctctcggatg atgcgggatg tcccgttccc gacggcatct actgggcccg
     2941 cccgtcgggt gccgggcccg aacgcaccgc gcggatcgtc gtccagcgaa actgacggcg
     3001 cggggtggcc gacggtccgg gccgcgaaac cagccgatcg ataacggggg cgccccggtt
     3061 cgtggataat gccgccccat ggacatcctt ctcctcctca aggccctcgt cctcggcgtc
     3121 gtcgaagggt tcaccgagtt ccttcccatc tcgtccaccg gtcatctcat cgtggcggcc
     3181 tcgctgctgc gcttcacccc cgcttcggcc gcggcgttcg agatcgccat ccagaccggc
     3241 gccatgctgg ccgtggtgtg ggagtaccgc gcgcgaatcg gccgaacggt ggccggcctc
     3301 gcttcacggc cggccgatcg gaaattcgcg ctgaacctgg cggtggcgtt cctgccggcc
     3361 atggtgttcg gacttgcgtt cggcaagctc atcaagcggc atctctttca cccggtgccg
     3421 gtggccatcg ccttcgcggt cggcggcgtg atcatcctcc tggtggaggg cgccatccgc
     3481 cggcgcgggg cgcctccccg ggtgggccgc gtggatgaca tgagcctgct cgacgcgctg
     3541 aaggtggggc tcgtccagtg cctggcgctc attcccggca cgagccgctc gggcgccacg
     3601 atcatcggcg gactggtttt cggcctgtcg cggccggcgg ccaccgagtt cagcttcttc
     3661 ctggcggtgc ccacgttgat ggcggcgggc gcgtattcgc tctacaagga gcgggacttt
     3721 ctcacggccg ctgacctgcc gctcttcctg gtgggcacgg tggtgtcgtt cgtgtcggcg
     3781 ctcgtggtga tccgctggct catccggtac gtggcgggac acgatttcac ggcattcgcc
     3841 tggtaccgga tcgcgttcgg ggcgatcatc ctctggacgg cgtggagcgg gctggtaagc
     3901 tggaatccat gatgtcgatc gactggcacg cgctcttcgt gccgcatggc tcgcttctcg
     3961 agatcgtggt gcggggcacg gtcatgtatc tcgtcctctt catcctgctg cgtgtgctgg
     4021 tgcgccgcca tgtgggggcg atgggcgtgc cggatctgct ggtcatcgtg ctgattgccg
     4081 atgcggccca gaacgcgatg gccggcgagt acaagtcgat caccgagggc gcgctgctct
     4141 gcgcggtgat catcggctgg agcgtgttgt tcgactggct ggcgtatcgg ttcaagccgt
     4201 tgcgcggaat cctggaagcg gatccactgc cggtcatcaa ggacggcgtg atgcttccgc
     4261 gcaacatgag gcaggagttg atcaccaggg acgagttgct gagccacctg cgcgagcagg
     4321 ggatcgacga tccggccgac gtggcactgg catgcatgga gcctgacggc gcgatcagcg
     4381 tgatcaagag ggacggcaag caggacgctc acccgccgaa gcggaagccg gtgcagtaag
     4441 tcgggatttt ttcaggctcg ggtcgagaca tcgccccgca tcagcagcca gtgcaacaac
     4501 cccgcgccgg caaatcccac gaaccacgcg taatcgtaca gcggcttcat caaggggatc
     4561 acgagccctc cccaggccag cgcgcaaccg aacagcgtgg cgaggaccgc cttccagttc
     4621 cagccccccg agtagcgata ggcgccgtcc ggcaggtaga ggtcctcgag tttcagctcc
     4681 ttcttccgga tcagccagta atcggcgatc aggacccccg cgatcgagcc gagccctccc
     4741 gagtagccga gcagccaggt gaagatgtaa cccgacgggt cggccaggag cttccagggc
     4801 tgcatcagga tgccgatcag gccggtgatg aggccgccgg tcttgaagtt gatggccttc
     4861 gggaaggcgt tggcgaagtc gttggccggc gacgcgacgt tggcggcgat gttgaccgcc
     4921 agtgtggcca ccaccgcggt gaacatcgag atgatgacga cgaacggcga cttgaattcg
     4981 cccaccagct tgaccgggtc ccagatggcg cgcccgtaga tgatgaccgt ggcgctggtg
     5041 atcaggattc ccatggccgc gaagaccgac atggtggtgg ggagggccac cacctgcccg
     5101 atcatctgct ccttctggct gcggccgaag cgggtgaagt ccggcatgtt cagcgacagc
     5161 gtggcccaga agccgatcat cgccgtgagg ctggggatga agaccggcag gaattcgccg
     5221 aacgtcttga acttgccggg ctgggccagg agcggcccga ggccgtcagc cttcatgatg
     5281 gcccaaccga ggagcaccgc cgtcatgatc aggacgaagg gcgccgccca gttctcgacc
     5341 tcacgcagaa gatccatgcc gcgatagatg atgaagatgt tgatccccca gaagaggagg
     5401 aaggagagcc acgccgtggt ggagtggcct gcgaaaccgg gaccgaggag gttggcccac
     5461 cccggaatca gtgtcgaaaa cagggtgtgc agcgcctccc cgccgatcca ggcctggatg
     5521 ccgaaccagc cacaggccac cagcgcccgc atcagggccg gcaggttcga accgagcgtg
     5581 ccgtaggcgg cccgcgcgaa gacggggaag gggatgccgt acttcgtgcc gggatgcgag
     5641 ttcagcagga tgggggcgag cacgatggtg ttgccgagca gcaccgtgat cagcgcctgc
     5701 agccagttca tgccggccgc catgaggccc gaagccagca tataggtcgg aatacagtgc
     5761 gccatgctga tccagagcgc cgcgtagttg taggtcgtcc agttgcgccg gccaatcggc
     5821 accggcgcca ggtcctcgtt cgacaggggg ctggccatca ccctcgaggt atccgccagc
     5881 tccacccgtc cgtccgcgtg tcgaatctcg cctgccacgg aaccctcccc tcgtcgacgc
     5941 gcaaatcatg cccggacttt ccagccacgc ccaatctgcc gaccgctttc ggcgccccgc
     6001 cgatcgccgg atggcgggaa aatacgacgg ctggggggtg caggggaatc ggccagggtg
     6061 atgatcacgg gtcatcgaat gtacctgttc cggaacaggt aacgaattgt gacctgggca
     6121 tgcgttgcgg gggcgcccgg agtgtacctg ttccggaaca ggtaacgaat tgtgacctgg
     6181 cctgtgtagc ggggggcgcc cgggcgtacc tgttccggaa caggtaacga attgtgacct
     6241 gggctgtgtt gcggggggcg cccggagcgt acctgttccg gaacaggt
//
LOCUS       DASVOD010000384         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_234906, whole genome shotgun sequence.
ACCESSION   DASVOD010000384 DASVOD010000000
VERSION     DASVOD010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234906"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF720_08810"
     CDS             <1..363
                     /locus_tag="VF720_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEX7879497.1"
                     /translation="SEGNPNVVLCERGVRTFADHTRNTLDLSSIPAVKKLSHLPIIAD
                     PSHGTGRRDKVAPMARAAMAAGADGLMVEVHHMPEKALSDGFQSLYPDQFDAMMETVR
                     RVRAVLEEFDPTIRGTGR"
     gene            complement(376..1062)
                     /locus_tag="VF720_08815"
     CDS             complement(376..1062)
                     /locus_tag="VF720_08815"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEX7879498.1"
                     /translation="MKRLVSLFLLVMVLMAAVVVAIRLRSRPAGSSAPLDPKVLPIAP
                     DLDSLPADSVWFGQVVHDREEQAAFFRGPDSPLEDRDRTDFQGLSYWPPDPAWRLTLR
                     FEPFADPDSVIILDTKGEERTFTRSGRFRFTREGREVTLTLYRQDYLKYWFLPFKDTT
                     AGTETYEVGRYVEPHDLGDGFFEVDFNRAYNPYCAYSHRWACPIPPKENELPIPIRAG
                     EKMFHAAAGS"
     gene            complement(1088..1663)
                     /locus_tag="VF720_08820"
     CDS             complement(1088..1663)
                     /locus_tag="VF720_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879499.1"
                     /translation="MRVSRFLVAGLSVVSLGALSGCTESNRLQLAEIEALERRVDKQM
                     RLVREQNEQINSNLKIIKAEMDTIKTVYLPAVQVALDSVAAKPEQARVQIIAEVDNRL
                     NTITASNRDFRTQVNDGIAASSKQLRDEVTKKIGEYDSRITKNEQFVNFYLAHQDSVN
                     QEFAVRIDKRPWYRSILGMWDDQQRQKTSAP"
     gene            complement(1835..>2980)
                     /locus_tag="VF720_08825"
     CDS             complement(1835..>2980)
                     /locus_tag="VF720_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879500.1"
                     /translation="WFSKVGALLSGYEIPNNRDIMAARSDSLLLSLLVGRVGPFFWPW
                     GLLLPLAVVGVIAPGGRRRWIVPLGAAVLYGASLIPFFVCDRFRLPVALFLAVPAGAG
                     ILALPSLVRGAGSIRRPAVLGGGAALVLSLAMWGVDTRVNAAESWHRLGEALYNAGRP
                     VAALDAFNEAVRMNPDDESVRLGRSWAILASADSLARADHPAEAAALDSLAGVELARA
                     AERLPDAWQAQYGYGHWLAAHGRGADAIPWLERAAGAQPSRAEVHRELGFAYEAAARW
                     REAGIALSHAVALGEETAEVHLSLGLAALHGGRADVAENNWRRAIELDPNHVRSLYNL
                     GLLEARRGNVEAAADLWRRALRVDPGNVLIQGQLNTLSPPGTTGTPR"
BASE COUNT          509 a          984 c         1032 g          455 t
ORIGIN      
        1 agcgagggga acccgaacgt ggtcctctgc gagcgggggg tgcgcacatt cgccgatcac
       61 acccgcaaca cgctcgacct gtcgtcgatc ccggcggtga agaagctttc gcacctgccg
      121 atcatcgccg acccgagcca cggcaccggg cggcgcgaca aggtggcccc gatggcccgc
      181 gccgccatgg ccgccggcgc cgacgggctc atggtggaag tccaccacat gccggaaaag
      241 gcgctctccg acggattcca gtcgctctat cccgaccagt tcgatgcgat gatggaaacg
      301 gtgcgccgcg tgcgcgcggt gctcgaggag ttcgacccga cgatccgggg aaccggccgc
      361 tagcctttcg gtccctcagc tccccgcggc ggcgtggaac atcttctcgc cggcacggat
      421 cgggatcgga agctcgtttt ccttcggcgg gatcgggcac gcccaacggt ggctgtaggc
      481 gcagtagggg ttgtaggccc ggttgaaatc cacctcgaag aagccgtccc ccaggtcgtg
      541 cggttccacg taacgcccca cctcgtaggt ttcagtcccg gccgtcgtgt ccttgaaggg
      601 gaggaaccaa tacttgagat agtcctggcg gtagagagtg agcgtcacct cgcgcccctc
      661 gcgcgtgaag cggaagcgcc ccgatcgcgt gaaggtccgc tcctccccct tggtgtcgag
      721 gatgatcacc gagtcgggat cggcgaacgg ctcgaagcga agtgtgaggc gccaggcggg
      781 gtcgggcggc cagtaggaaa gcccctggaa gtcggtgcgg tcacgatctt ccaggggcga
      841 atcagggccc cggaagaagg ccgcctgttc ttctcgatcg tgcaccacct ggccgaacca
      901 gaccgagtcg gccgggagtg aatcgagatc gggtgcgata gggagcacct tggggtcgag
      961 cggcgcgctc gagcccgccg gacggctccg caggcggatg gccaccacga cggcggccat
     1021 cagcaccatg acgagcagga agaggctgac gagccgtttc atcgggtgat gtgcgggagc
     1081 gacggcgtca gggcgccgac gtcttctggc gctgctggtc gtcccacatg ccgaggatcg
     1141 accggtacca cgggcgcttg tcgatgcgga ccgcgaactc ctggttcacc gagtcctggt
     1201 gcgccaggta gaagttgacg aactgctcgt tcttggtgat gcgggagtcg tactcgccga
     1261 tcttcttcgt gacttcgtcg cggagctgct tgctcgaagc ggcgatcccg tcgttgacct
     1321 gggtgcggaa gtcccggttg ctggccgtga tggtgttcag ccggttgtcg acctcggcga
     1381 tgatctgcac gcgggcctgc tcgggcttgg cggccacgga atcgagggcc acctggacgg
     1441 ccgggaggta caccgtcttg atggtgtcca tttcggcctt gatgatcttg aggttgctgt
     1501 tgatctgctc gttctgctcc cgcaccaggc gcatctgctt gtcgacgcgg cgttcgagcg
     1561 cctcgatctc cgccagctgc aacctgttgc tctcggtgca gccggacagg gctccgagtg
     1621 aaacgaccga cagtccggcc acgagaaacc ggctcacgcg catggggatt ctcccttggt
     1681 accagcccgg aaacgggatg aggttggtcg agaaaaccag aggggacaac aacatcgtgc
     1741 gggcggcaac tgccggcacg cgaacgacga aaggatgata ggggaggccc cgaaggctgt
     1801 caacgaaatg gccattttcg ttggcatgcc gccgtcagcg gggcgttcct gtcgtcccgg
     1861 ggggcgaaag ggtgttcaac tgcccctgga tcaggacatt tcccggatcg acgcggagcg
     1921 cccgccgcca cagatccgcc gccgcctcga catttccgcg acgcgcctcg aggagcccca
     1981 ggttgtagag gctccggaca tgattcggat cgagctcgat ggcgcggcgc cagttgttct
     2041 ccgccacgtc ggcccgaccg ccgtgcaggg cggcgaggcc gagcgagagg tgtacctcgg
     2101 ccgtctcctc gccgagtgcc acggcatgac tcagggcgat gccggcctcg cgccagcgcg
     2161 cggccgcctc gtacgcgaac ccgagctccc tgtgcacctc ggcccgcgat ggctgggcgc
     2221 ctgccgctct ttcgagccag ggaatggcat cggctccccg gccgtgcgcg gccagccagt
     2281 gaccgtagcc gtactgggcc tgccaggcat ccggaagtcg ctcggcggcc cgggccagtt
     2341 ccacgccggc caggctgtcg agcgccgcgg cttcggcggg gtggtcggct cgcgccagtg
     2401 aatccgccga ggccaggatg gcccacgagc gcccgagccg caccgactcg tcatcggggt
     2461 tcatgcgcac cgcttcgttg aaggcatcca gcgccgccac cggccggccg gcgttgtaga
     2521 gcgcctcgcc aaggcgatgc cacgactcgg cggcgttgac gcgggtgtcc acaccccaca
     2581 tcgccagaga gaggacaaga gcggcgccgc cgccgaggac ggccggcctg cggatcgacc
     2641 ccgctccccg cacgagcgag gggagcgcga ggattcccgc cccggccggc acggccagga
     2701 agagggccac cgggaggcga aaccgatcac acacgaagaa ggggatcagg gaggcgccat
     2761 agagcaccgc ggcgccaagc ggcacgatcc agcgccgccg accgcccggg gcgatcacgc
     2821 cgaccacggc aagggggagc agcaatcccc acggccagaa gaacggaccc acgcgcccga
     2881 caagcaaaga gaggagcagc gagtccgatc gcgccgccat gatgtcccgg ttgttcggga
     2941 tctcgtaacc cgaaaggagt gcgccgacct tcgagaacca
//
LOCUS       DASVOD010000385         2857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_755869, whole genome shotgun sequence.
ACCESSION   DASVOD010000385 DASVOD010000000
VERSION     DASVOD010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755869"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VF720_08830"
     CDS             <1..842
                     /locus_tag="VF720_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879501.1"
                     /translation="QGAENATGSGADLSTVDQTLVTNAIEGAGETVQMMNPGGAPRLF
                     LGRFPASVPEGCNYDAATGRFNCAPVADEHGPTLERSYAYLDGAGNPQGAYDAAGTAA
                     INFQSRVNGAIGRGQVTGTVDHQRDLTASGLSGAETTWTWNGTGSGTSHEEGIFGPPP
                     GRGRGHGPGDGAGMGPGAGHGPGAGKGAGVAMSIDVTSSMAVSNVVVPYPPAQGSWPL
                     SGTITQSMTATITGGPHDGEVHERAAILTFNGTQYATLTVGDETRTIDLANPPGPPHR
                     GPR"
     gene            complement(912..1472)
                     /locus_tag="VF720_08835"
     CDS             complement(912..1472)
                     /locus_tag="VF720_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879502.1"
                     /translation="MRHRRSAPLGLLTAAALLLLLPAPLPADDAAPTPLDHFGKVLAR
                     HRIVLGSHQRVCVAETFYGPGATLDFGLIEGRRHDRLYFRNSRRILQEWTYPGVGLPD
                     SAIAWGKSFANLFTANDTLFVELFQVGDPNWASGQRAVRMEALFQKWIRRAFNQGDWA
                     EAEALGIGAERFKGPPPELVCPLGGG"
     gene            complement(1436..2425)
                     /locus_tag="VF720_08840"
     CDS             complement(1436..2425)
                     /locus_tag="VF720_08840"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7879503.1"
                     /translation="MTLSSNPWSASSRSEPSMLLKAGRRIKEQSWHNWLLLLTTLIVT
                     TIGLGAATASLLTDRDRTPWAWAGSEWVLLAGLSLIVALFGSYLAFQRRKLSRLENEL
                     DRTREEAIVTTTRHYSRLMALCRVNQTMANENDIEAVFASMTQLCFSYFDCDRVSVML
                     VDRGTGELVVRAAMGHQGAAEIIGSRQQPGRGIAGWVARHREPVVLGEVVEPGRFEGF
                     EPGKSGLTAAIVVPILVRDELVGVISLSSQKPGIAYSNEDLRALEIFAGNAGLCLRHT
                     EQAGWMRQTIWRLEGSLREHELSERMGGFVCPPPTPAGSVERCDTDDRLRSAS"
     gene            2622..>2857
                     /locus_tag="VF720_08845"
     CDS             2622..>2857
                     /locus_tag="VF720_08845"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017990399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX7879504.1"
                     /translation="MTTPDSGLVVGDDQASRCWWGASNEDYARYHDREWGFPVTDDTR
                     LFEKICLEGFQAGLSWLTILRKRENFRAAFADFDV"
BASE COUNT          485 a          973 c          969 g          430 t
ORIGIN      
        1 accagggcgc cgaaaatgcc accggcagtg gggccgacct ctccacggtc gaccagacgc
       61 tggtaaccaa cgcaatcgaa ggcgccggcg aaacggtgca gatgatgaat ccggggggcg
      121 caccgcgcct cttcctgggg cgcttcccgg cctcggtgcc cgaggggtgc aactacgacg
      181 ccgccaccgg ccggttcaac tgtgctcccg tggccgacga acacggcccg acgctggaac
      241 ggagctacgc ctacctcgac ggcgccggaa atccgcaggg ggcctacgac gcggccggca
      301 ccgcggccat caatttccag agccgcgtga acggcgcgat cgggcggggg caggtgaccg
      361 gcaccgtgga ccaccagcgc gacctcaccg ccagcgggct gtcgggcgcc gaaaccacct
      421 ggacgtggaa cggcaccggt tccggcacct ctcacgaaga ggggatcttc ggtccgcctc
      481 cgggacgcgg tcgggggcat ggcccgggcg acggcgccgg tatggggccg ggggcgggac
      541 acggcccggg cgccgggaag ggcgcgggtg tggccatgag catcgacgtc acgagctcga
      601 tggccgtctc gaacgtggtg gtgccctatc cccccgcgca aggctcctgg cccctctcgg
      661 ggaccatcac gcagtcgatg accgccacga tcaccggcgg gccgcacgac ggcgaggtgc
      721 acgagcgtgc ggccatcctc accttcaacg gcacgcagta cgccacgctc acggtcggcg
      781 atgagacccg cacgatcgac ctggccaacc cgccggggcc gccgcaccgc ggtccgcgct
      841 gaccgaataa cagttctccg aatagaccaa catccaaccg agccccgccc tccgggcggg
      901 gctttctgtc atcacccgcc gccgagcgga cagaccagtt ccggaggcgg gcccttgaag
      961 cgttccgctc cgatgcccag cgcttcggcc tcggcccaat ccccctggtt gaaggcccgg
     1021 cggatccact tctggaagag agcctccatc cgtaccgccc gctggcccga cgcccagttc
     1081 gggtcgccga cctggaagag ctccacgaag agcgtgtcgt tggcggtgaa gaggttggcg
     1141 aaactcttcc cccaggcgat cgcgctgtcg ggaagcccca cgccggggta ggtccactcc
     1201 tggaggatgc ggcggctgtt ccggaaatag agccggtcgt gccggcgccc ctcgatcagg
     1261 ccgaaatcga gcgtcgcccc ggggccgtag aacgtttcgg ccacgcagac gcgctggtgc
     1321 gaaccgagga cgatgcgatg ccgggccagg accttgccga agtggtcgag cggcgtgggc
     1381 gcggcgtcgt cggccgggag cggagcgggg aggagaagga gtagggcggc ggccgtcagg
     1441 aggccgagcg gagccgatcg tcggtgtcgc acctctcgac actaccagcc ggcgtcggtg
     1501 ggggacaaac gaatcccccc atgcgctccg agagttcgtg ctcgcgcagc gagccttcca
     1561 ggcgccagat cgtctggcgc atccagccgg cctgctccgt gtgacggagg cagagccccg
     1621 cgttgccggc gaaaatctcg agtgccctca ggtcttcgtt ggagtaggcg atgccgggct
     1681 tctggctgga gaggctgatg acgccgacca gctcgtcgcg caccaggatc ggcacgacga
     1741 tggccgcggt gaggccgctc ttgccgggct cgaatccctc gaagcggccc ggctcgacga
     1801 cttcgcccag gaccaccggt tcacgatggc gggccaccca gccggcgatg ccgcgtccgg
     1861 gctgctgccg agacccgatg atctccgcgg ccccctggtg ccccatggcg gcgcgaacca
     1921 ccagctcgcc ggtgccgcga tcgaccagca tcaccgacac gcggtcgcag tcgaagtagc
     1981 tgaagcagag ctgggtcatc gaggcgaaga ccgcttcgat gtcgttctcg ttggccatcg
     2041 tctggttcac ccgacagagg gccatcaacc gtgagtagtg cctcgtggtg gtgacgatgg
     2101 cctcttcgcg cgtccggtcg agctcgttct cgaggcgcga caacttgcgg cgctggaagg
     2161 ccagatacga gccgaatagc gccacgatca gggacaatcc cgcaagcagc acccattcac
     2221 tccccgccca cgcccatggg gtgcgatctc ggtcggtcag gaggctggcc gtggccgcgc
     2281 cgagtccgat ggtggtgacg atcagagtgg tgaggaggag aagccagttg tgccagctct
     2341 gctccttgat ccgacgcccg gctttcagca gcatggacgg ctcggatcgg ctcgatgccg
     2401 accaggggtt ggacgacagg gtcatcctct ctctccagat ttcgaagggg tcgcgtggac
     2461 gcatcgcggt atttttcgac gccgcacgcc ctaacctttg gacttccctg aatatcgccc
     2521 gaaggaggcc cggacttgag cgggaattca agctccactg cacaattgtc atcttacgtg
     2581 aggcaaataa ccctccctgg gcgggatgac gcagggatcc catgacgacc cccgattccg
     2641 gcctcgtggt cggtgacgac caggcgtccc gctgctggtg gggcgcctcc aatgaagact
     2701 acgcgcgcta ccacgaccgc gagtggggat ttcccgtgac ggacgacacc cgcctcttcg
     2761 agaagatctg cctcgaggga ttccaggccg gcctctcatg gctgaccatt ctgcgcaagc
     2821 gcgagaactt ccgcgcggcg ttcgccgatt tcgatgt
//
LOCUS       DASVOD010000386         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_234961, whole genome shotgun sequence.
ACCESSION   DASVOD010000386 DASVOD010000000
VERSION     DASVOD010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234961"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1210)
                     /locus_tag="VF720_08850"
     CDS             complement(17..1210)
                     /locus_tag="VF720_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879505.1"
                     /translation="MTVTLLAHLAELDKRRLYLAEGCSSLFSWCTEVLHYSEAAAYNR
                     IKCARAIRRFPLVLERLADGSVHLTAVRLLAPCLTEANHREVLDRARHLGRRAVEELV
                     AELRPRPDAASVIRRVAESTSVAPGATLLLELGPAPARQAAQVPHSSSRGVEAAVPRT
                     GSASVASAPAATTPLSPGRYRFQFTADHEMHDDFLQARELLRHQFPGGDAAAIMKLAL
                     RRLVADLRKRKFAQMDRPKPANGRPVGPTTTTGRHIPSAVRRHVAERDGARCTFVSQS
                     GRRCSETGQLEFHHHEPFALGGAATIENISLRCRAHNAHEARETYGSKAALKHRRGAG
                     EVREIGPGYRTTPTTGTFRAGPGPKAPDLPVPHRLVPGPVGNRKARPDRPGEARRSPS
                     ARLPP"
     gene            1714..>3309
                     /locus_tag="VF720_08855"
     CDS             1714..>3309
                     /locus_tag="VF720_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879506.1"
                     /translation="MTRCTPRTRFLLPIASLAFSALAIATDFVRPASAGISPGDWGDA
                     PEGYECYATPPTTGRFPTCIGGSTGHILNNRPDGQPNAFFGPSWENDGAGNGGNCVPE
                     FNGDETQFDGDAGLTIVDAWSNGGGFDYVISGTGTPVPLGQVYGTSTPGVNFDMEIHN
                     QMPGTHYLNLLVDWDRNGRWEPYRTWDSNGSPATEWPIKDLAVPAGFNGMLSQLTTNP
                     IPVGPFPGLVWCRFVLTPNQLFPSLGLGWNGSGTFNSGESEDYLLQVRGNSANFGDYG
                     DAPEGSTAYPQFGINGSFPTVRWNAEAGFIQHHSNGLLAFGQFVDPENDGNAGATSTN
                     PPNQDECVGGTDAGLIAPSAYTIDDGSTMPCDPLSTVPLGGGCGTVTVGPNLDIAIRN
                     INQGTCYFNLLIDLDYSGSWGGLTSCPLGGDTDEHVFQNIVVPNNFNGELSDLLPGAT
                     FTITSPPGYTWARFTLTSIPVPANWDGSGDFANGETEDYLFRIEGPGLGDYGDAPENA
                     LAYPNSGYWGAFATCAADGFPHFV"
BASE COUNT          565 a         1133 c         1044 g          567 t
ORIGIN      
        1 aggttctcga agcgagtcat ggggggagtc tagcagaggg gctgcgacgt gcttcgccag
       61 gtcggtccgg gcgggccttc cggttgccga ctggtcccgg gaccagtcgg tgcgggaccg
      121 gcaggtcggg agctttgggt cccgggccgg cgcggaacgt tccggtcgtc ggagttgtcc
      181 gatatccagg cccgatctcg cgcacctccc ccgccccgcg gcgatgtttg agtgccgcct
      241 tggacccgta cgtttcccgc gcttcatgcg cattgtgggc gcgacagcga agcgagatgt
      301 tctcgatggt ggccgcgccc cccagggcga acggctcgtg atgatgaaac tccagttgtc
      361 cagtttccga gcatcggcgg cccgactggc tcacgaaggt gcaacgggcg ccgtcgcgtt
      421 cggcaacgtg tcgtcggacg gccgacggga tgtggcgccc ggtcgtcgtc gtcggcccta
      481 cgggccgccc gttcgcaggc ttgggccgat ccatttgcgc aaattttctt ttccgcaagt
      541 cggcgaccaa tcgacgcagc gccagtttca tgatcgcggc cgcatcgcca ccgggaaact
      601 ggtgacgcag cagctcgcgc gcctgaagga agtcatcgtg catctcatgg tcggccgtga
      661 actggaagcg atatcggccc ggggaaaggg gagtcgtcgc ggcaggagcg gaggccacag
      721 aagccgagcc ggtccggggt accgctgctt cgactccccg gctcgaggag tgcggcactt
      781 gcgccgcctg cctggccggc gccggtccaa gctccagcag cagggtcgcg ccgggcgcaa
      841 cagaggttga ctccgccacg cgccggatca ccgaggccgc atccggcctc ggcctcagtt
      901 ccgccaccag ttcctcgacc gcccgtcttc caaggtgacg tgcccgatcc agcacttcgc
      961 ggtgattggc ttcggtcagg cagggcgcca ggagtcgaac agccgtcagg tgcaccgagc
     1021 catcggccag tcgctcgaga acgagcggaa accgacgaat ggcccgcgcg cacttgatgc
     1081 ggttgtacgc ggcggcctcg gagtaatgga gaacctcggt gcaccaggag aacagcgaag
     1141 agcagccttc cgcgaggtac aagcgtcgtt tgtccagctc ggccaggtgc gccagcaggg
     1201 tcaccgtcac ccgccgctcg ctgcgcgcga gttccgtaac gcgggccacg agtgcggggc
     1261 tggagagccg tgtgggtaat gtgagtgtcg ttatcatgtc atggttatag cacgcacaat
     1321 ttctcgctcg tgttgatcca tgccaaacat aagaatgcgt cgggacgatg tttgagtcgt
     1381 gatgcgcgcc atcagcgcac accggacccc cggtcgcggc ctgggccccg ttccattcgg
     1441 cgttagcggg ctcggaattc gatgccggaa tttgaagtca agaaaattct caagaagttg
     1501 tttttccgga gcaacaggtc ccgggacccg ttggttgcga acgcgtacat gacatgcatg
     1561 attatgcaga caacgacatc gcaaccggct atcatggtcg cccacctgcc cggtcccccc
     1621 ggcgggactt caccacctgc atgacatcca agcgtcgggc cggccgcccc gaggtcggag
     1681 ttcgacgccc atcgcgtccg accggaggcc gccatgaccc gatgcacgcc gcgcaccagg
     1741 tttctgctcc cgattgccag ccttgcattc agtgcacttg ccatcgccac cgatttcgtc
     1801 cgccccgcct cggccggcat cagccccggc gactggggcg acgcgccgga aggctacgag
     1861 tgctatgcca cgccgcccac caccggtcgc tttcccacct gcatcggcgg atccaccggg
     1921 cacatcctga acaatcggcc ggatggccaa ccgaacgcct tctttggccc ttcctgggag
     1981 aacgacggcg ccggtaacgg cggcaactgc gtgccggagt tcaacggtga cgagacgcag
     2041 ttcgacggcg acgccggcct cacgatcgtc gatgcctggt cgaacggcgg gggtttcgat
     2101 tacgtgatca gcgggacggg aactccggtc ccgctcggcc aggtgtacgg cacctccacg
     2161 ccgggcgtga atttcgacat ggaaatccac aaccagatgc cgggcactca ctacctcaac
     2221 ctcctggtgg attgggaccg caacggtcgc tgggagccgt atcgtacgtg ggacagcaat
     2281 ggctcgccgg ccaccgagtg gccaatcaag gacctcgccg tcccggccgg attcaacggc
     2341 atgctttccc agctcacgac caacccgatc ccggtcggcc cctttcccgg cctcgtctgg
     2401 tgccgcttcg tcctgacccc gaatcaactc ttcccgtccc tcggcctcgg ctggaacggc
     2461 agcggcacgt tcaacagcgg cgaatccgag gattacctcc ttcaggtcag aggcaactcg
     2521 gccaacttcg gcgactacgg cgacgcgccc gaaggctcga ccgcctaccc ccagttcggc
     2581 atcaacgggt cctttccaac cgtgcggtgg aacgccgagg ccggcttcat tcagcaccac
     2641 tcgaacggcc tgctcgcctt cggccagttc gtggatccgg aaaacgacgg caatgcgggc
     2701 gctaccagta cgaacccgcc gaaccaggac gagtgcgtcg gcggcaccga tgccgggctc
     2761 atcgccccgt cggcctacac catcgacgac ggatccacga tgccgtgcga tccactctcc
     2821 acggtgccgc tcggcggcgg ttgcggaacg gtgacggtcg gcccgaacct cgacatcgcc
     2881 atccgcaaca tcaaccaggg gacgtgctac ttcaacctgc tgatcgacct ggactactcg
     2941 ggctcgtggg gcggcctcac cagttgcccg ctcggcggcg acaccgatga acatgtgttc
     3001 cagaacatcg tcgtgccgaa caacttcaac ggcgagttgt cggacctgct gcccggggcc
     3061 acgttcacga tcacctcgcc gcccggctat acgtgggccc gcttcacgct gacgtcgatc
     3121 ccggtgccgg ccaactggga cggctccggc gatttcgcca acggcgaaac cgaggactac
     3181 ctcttccgta tcgagggccc cggcctcggc gactacggcg acgcgccgga gaacgcgctg
     3241 gcctatccca attcgggcta ctggggcgcc ttcgccacct gcgccgccga tggcttcccg
     3301 cacttcgtg
//
LOCUS       DASVOD010000387         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_755927, whole genome shotgun sequence.
ACCESSION   DASVOD010000387 DASVOD010000000
VERSION     DASVOD010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755927"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1954)
                     /locus_tag="VF720_08860"
     CDS             complement(152..1954)
                     /locus_tag="VF720_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX7879507.1"
                     /translation="MGKSDVNVKPIASRDRREFLRWCAGIGLTGAALAEAGLSMAQAP
                     ATGPAPIADDALKGAAHLAALDFTPAERELMAAGAADNRAGIERMRKVALANDVPPAL
                     IFTPIQPGVVNLGRPRAEITGPFDAPRNGNGAGPSRSATVASPRGQHSAPIRPTNPAD
                     LAFSTVAELAGLLESRKITSRELTEIYLDRIGRYDKQLEAVITLTADLARAQADAADA
                     ELKAGRYRGALHGIPYGAKDLLATRGIATTWGAEPFRGQVPREDAAVIRRLNEAGAVL
                     ISKTSLGALAYGDVWFGGTTRNPWKLDQGSSGSSAGSASGTAAGLFPFAIGTETLGSI
                     VSPCTRCGTTGLRPTFGRVSRAGAMALSWSMDKVGPIARNVGDTALVFAAIHGADGVD
                     NAVHDAPFDADLGRVVPDWTRLRIGYDRAAFDETRDDKSFDDAVLETMRRLGANLVPV
                     TLPDLPVSDTLLLLECEAAAAFDELTRTNQDEQLKRQTAQAWPNIFRTARLIPAVDYI
                     QANRIRTLLMREMDRMMANVDVYLSPSFGGSLRTTNLTGHPTVVLPNGFRADGTPTST
                     TFIGRLFGEEAVLAVAKAYQDATEWHRRRPGGFA"
     gene            complement(1956..>2193)
                     /locus_tag="VF720_08865"
     CDS             complement(1956..>2193)
                     /locus_tag="VF720_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879508.1"
                     /translation="VLFEARIFLSERALLLARAGRDAEARRQVSDNVRRWRRDPVVLR
                     KACETLWSMSHSDEALLLYDEVLQLTRMTERPGG"
BASE COUNT          316 a          711 c          809 g          357 t
ORIGIN      
        1 tccacatggc ggactcaaag gccaaacgcc cttcagcaga ctgcggaggt ttggtgaata
       61 acgtgtctag aaatcacagc tagtcagcca gccaacaggt tccggaacct gttggcggcg
      121 ccagcggcgc cgctccactg gcccttagac atcaggcaaa cccccccggc cgccgccgat
      181 gccactccgt ggcgtcctgg tacgcctttg ccacggccag caccgcctct tcgccgaaca
      241 gccggccgat gaacgtggtg gatgtcggcg tgccgtctgc gcgaaatccg tttggcagca
      301 ccaccgtcgg gtgtccggtg agattggtcg tgcggagcga gccgccgaac gatggcgaga
      361 ggtagacatc cacattggcc atcatccggt ccatctcgcg catgaggagg gtgcggatgc
      421 ggttggcctg gatgtaatcg accgctggga tgagtcgcgc cgtgcggaag atgttgggcc
      481 aggcctgggc ggtctggcgc ttgagctgct cgtcctggtt ggtgcgggtg agttcgtcga
      541 acgcggctgc ggcctcgcat tcgagcagga ggagcgtgtc gctgaccggg aggtcgggga
      601 gggtgaccgg cacaaggttg gcgccgaggc ggcgcatcgt ttcgaggacg gcgtcgtcga
      661 acgacttgtc gtcgcgggtc tcgtcgaagg cggccctgtc gtagccgatc cgcaggcgtg
      721 tccagtcggg cacgacgcgg ccgaggtcgg catcgaacgg tgcgtcgtgg acggcgttgt
      781 ccactccgtc ggcgccgtgg atggcggcga agacgagggc ggtgtcgccc acgttccggg
      841 cgatgggtcc gaccttgtcc atgctccacg acaacgccat ggcgcccgcc cggctcacgc
      901 ggccgaacgt ggggcgcagc cccgtggtgc cgcagcgcgt gcaggggctc acgatcgaac
      961 ccaacgtctc ggtgccgatg gcgaagggga atagtccggc ggccgtgccg ctggccgaac
     1021 ccgccgatga gccgctcgat ccctgatcga gcttccaggg gttcctggtc gtgccgccga
     1081 accagacatc gccgtaggcc agggcgccga gggacgtctt ggagatcagg acggcaccgg
     1141 cctcgttcag gcgccggatg acggcggcgt cctcgcgggg tacctggccg cgaaatggct
     1201 cggcgcccca cgtggtggcg atgccgcggg tggccaggag gtccttggcg ccgtacggga
     1261 tgccgtgcag cgccccgcgg tagcgcccgg ccttgagttc ggcgtcggcg gcgtcggcct
     1321 gcgcgcgggc caggtctgcc gtgagcgtga tgacggcttc gagctgtttg tcgtaacggc
     1381 ctatccggtc gagatagatc tccgtgagtt cgcgcgacgt gatttttcgc gattcgagga
     1441 gcccggcgag ctccgccacc gtcgagaacg cgaggtcggc cgggttggtc ggccggatcg
     1501 gtgcgctgtg ctgcccacgt ggactggcga ctgtggccga gcgcgacggg ccggcgccgt
     1561 tcccgttcct cggcgcatcg aacggccccg tgatctccgc gcgcggccgg ccgaggttca
     1621 cgacgccggg ctggatcggc gtgaagatga gtgccggcgg cacgtcgttg gccagcgcca
     1681 ccttccgcat ccgctcgatc cccgcgcggt tgtcggcggc gccggcggcc atcaattccc
     1741 gctcggccgg cgtgaagtcg agggcggcca ggtgggccgc ccccttcaag gcgtcatcgg
     1801 caatcggtgc gggcccggtg gccggggcct gggccatcga gaggcccgct tcggccagcg
     1861 cggcaccggt caggccgatg ccggcgcacc agcggaggaa ctcgcgccga tcacgtgatg
     1921 cgatcggttt cacgttcacg tcggacttgc ccatctcatc ccccgggtcg ttcggtcatg
     1981 cgggtcaact gcagaacctc atcgtacaag aggagcgcct cgtcggagtg gctcatcgac
     2041 cagagcgttt cgcaggcctt ccggaggacg acgggatcgc ggcgccagcg ccggacgttg
     2101 tcggatacct ggcggcgggc ctcggcgtcg cggcccgcgc gggccaggag gagcgcccgt
     2161 tcggaaagga agatgcgcgc ctcgaagagc acc
//
LOCUS       DASVOD010000388         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_703388, whole genome shotgun sequence.
ACCESSION   DASVOD010000388 DASVOD010000000
VERSION     DASVOD010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703388"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1385)
                     /locus_tag="VF720_08870"
     CDS             complement(3..1385)
                     /locus_tag="VF720_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX7879509.1"
                     /translation="MSSIQPTHVDTLKAGFAGEIFLPGTEGYEKSRAIWNGMIDKKPG
                     AIARCANTKDVVAAVNLAREHGLTLAVKGGGHNIAGSALCNDGLVIDLSGMKRVTVDT
                     AARRATVQGGALLGDFDAATLPHGLATPLGINSTTGVAGLTLGGGFGWLSRKYGMSVD
                     NLESAEVVTADGQVRHVSASENADLFWAIRGGSGNFGVVTQFEFRLHPVGPDVLTGLI
                     VYPIAQAAGVLRKYRDFVANAPDELSVWSVLRLAPPLPFLPEAAHNKGMIALAFCYAG
                     DPAVGEPLVQPLRSFGTPVGEHIGVQPYTAWQQAFDPLLAPGARNYWKSHNFSTIPDG
                     LIDAVVSAIDTLPSPQCEIFFGAIGGATMRAAVDATAYPNRDALYVMNVHGRWDTAAE
                     DERGVKWARDFFKASAPFATGSVYVNFLTDEETDRVAAAYGPNYGRLASIKKTYDPTN
                     LFCTNMNIKP"
     gene            complement(1526..>1787)
                     /locus_tag="VF720_08875"
     CDS             complement(1526..>1787)
                     /locus_tag="VF720_08875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7879510.1"
                     /translation="VIIDHGNDEFSALMHMQLGSVTVKVGDKVTAGQVVGKLGHSGDA
                     FGVHLHYQLQDGPKLFQASSLPIMFKNVRETSFSRGAYLTPH"
BASE COUNT          304 a          594 c          583 g          306 t
ORIGIN      
        1 ccctacggct tgatattcat gttcgtgcag aacaggttcg tcgggtcgta cgtcttcttg
       61 atcgacgcca gccgaccgta gttcggcccg tacgcggccg ccacgcgatc ggtctcctcg
      121 tccgtcagga agttgacgta cacgcttccc gtggcgaacg gcgccgacgc cttgaagaag
      181 tcgcgcgccc acttcacgcc gcgctcgtcc tcggcggcgg tatcccagcg gccatgaacg
      241 ttcatcacgt acagcgcgtc gcgattgggg tacgcggtgg catccaccgc ggcccgcatc
      301 gtggcgccgc cgatcgcgcc gaagaagatt tcgcactggg gcgagggcag cgtgtcgatg
      361 gcggaaacca ccgcgtcgat caatccgtcc ggaatcgtcg agaagttgtg ggacttccag
      421 taattccgtg cccccggcgc gaggagtgga tcgaacgcct gctgccaggc ggtgtagggc
      481 tgcacgccga tgtgttcgcc gaccggcgta ccgaacgagc gcagcggctg cacgagcggc
      541 tcgccgaccg caggatcgcc cgcgtaacag aatgccaggg cgatcattcc cttgttgtgc
      601 gcggcttcgg gcaggaacgg cagcggcggc gcaaggcgca ggacgctcca cacggaaagc
      661 tcatcgggcg cattcgcgac gaagtcgcga tacttccgga gcacgccggc cgcctgcgcg
      721 atcgggtaca cgatgagacc ggtcagcaca tcgggcccca ccggatgcag gcggaactcg
      781 aactgcgtca ccacgccgaa attgccgctg ccgccgcgga tcgcccagaa gagatcggcg
      841 ttctccgacg cgctgacatg tcgcacctgt ccgtcggccg tcacgacctc ggccgactcg
      901 agattgtcga ccgacatccc gtacttgcga ctcaaccagc cgaacccgcc gccgagcgtg
      961 agaccggcca cgccggtcgt cgaattgatg ccgagcggcg tggccaggcc atgcggcaac
     1021 gtggccgcgt cgaaatctcc caggagcgca ccaccttgaa cggtggcccg gcgcgccgcc
     1081 gtatcgaccg tcacgcgctt cattcccgac aggtcgatga ccagcccgtc gttgcacaac
     1141 gcgctgccgg cgatgttgtg gccgccaccc ttcacggcca gcgtgaggcc gtgttcgcga
     1201 gccagattga ccgcagccac gacatccttc gtgttcgcgc accgggcgat ggcccccggc
     1261 ttcttgtcga tcatcccgtt ccagatggcg cgggactttt cgtaaccctc ggtgccgggc
     1321 aggaagattt cgccggcgaa gccggccttg agtgtgtcga cgtgggtggg ttgaatcgag
     1381 gacatgtccc gctccttgtg cgatgtcgag ctcgcggccc cggcgccgga ggcccggcgc
     1441 gttttcgggg aaattcgctg tcgagtgtcg gagcggaccc actcgaatgc aagggcggat
     1501 cggcggccgg aggcgagccc gggaatcagt gcggggtgag gtatgccccg cgcgagaagc
     1561 tcgtttcccg gacattcttg aacatgatcg gtaaggagct ggcctgaaac aacttggggc
     1621 cgtcctgaag ttgatagtgg aggtgcacgc caaaggcatc gccggaatgg ccgagcttgc
     1681 ccaccacctg cccggccgtc accttgtccc ctaccttcac cgtgaccgag cccagctgca
     1741 tgtgcatcag ggcgctgaac tcgtcgttcc cgtggtcgat gatcacg
//
LOCUS       DASVOD010000389         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_390743, whole genome shotgun sequence.
ACCESSION   DASVOD010000389 DASVOD010000000
VERSION     DASVOD010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390743"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF720_08880"
     CDS             complement(<1..225)
                     /locus_tag="VF720_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEX7879511.1"
                     /translation="MSERREEELKPDTLAVHGAAFRDDAERSHVPPIHLATVFEFGSV
                     DEMAATFRGERKGWVYTRYGNPTLDLVERHV"
     gene            complement(239..892)
                     /locus_tag="VF720_08885"
     CDS             complement(239..892)
                     /locus_tag="VF720_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879512.1"
                     /translation="MIRLALGALLLGVLANAAEAADPKLLFHARRASMAQPCQANLSR
                     CQDVDVLGQLYAPSGAGNYYVYLVAAGIDPAQGLASVSFGIDYDAVTVSGVDLFTWNL
                     CADSEEPGTGWYQEAHSSNRISWNAESCPAESLVVGGYFYMTCYTASFLTVGPVGDDP
                     VTFRDCQSQDVTLPQSSLGHVAFGATGGCNPCLGPCDKVAVTATTWSGIKTLNGPDR"
     gene            complement(889..1608)
                     /locus_tag="VF720_08890"
     CDS             complement(889..1608)
                     /locus_tag="VF720_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879513.1"
                     /translation="MRCRQLVVGIIALGIGFAFAAPASATSFDNRPKLLLHVKSKTTK
                     NPCSFGSVNDCQASTVVKANLTNVNDPNTFYFVYLLAVKASIPNLAGVQCGIQYEGGA
                     DPNGGQVPINVASWSNCATLEFPTPGADAWPAPGGGNLITWDSMNACQTGEVGVAGYF
                     YLGAYAPATMRLTTRPVDNKAALADCSSVESEPLLTKDLGFVAFSAGAVTEGCNPCVA
                     ECMPVPVAPTSWSSIKSLLGR"
     gene            complement(1822..>2091)
                     /locus_tag="VF720_08895"
     CDS             complement(1822..>2091)
                     /locus_tag="VF720_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879514.1"
                     /translation="CQTGAVATAGYFYLTAYAPSTLKLIPRPIDMAAKVADCGSLEIP
                     LAASELGWASFSAGATVPGCNPCVQNCGIVGVEPSTWSSIKTLNH"
BASE COUNT          355 a          645 c          727 g          364 t
ORIGIN      
        1 cacgtgccgc tcgaccagat cgagcgtggg gttgccgtag cgtgtgtaga cccagccctt
       61 gcgttcgccc cggaacgtgg cggccatctc gtccaccgat ccgaattcaa aaacagtagc
      121 caggtggatg ggggggacat gtgagcgctc ggcatcatcc cggaaggcgg cgccgtgcac
      181 ggccagtgtg tcgggtttca gctcttcctc gcgccgttcg ctcatccgcg tggcccgttc
      241 atcgatcggg gccgttcagc gtcttgatgc cgctccaggt cgtggccgtg acggccacct
      301 tgtcgcatgg gccgaggcag ggattgcagc cgcccgtcgc gccgaacgcc acgtggccga
      361 gcgacgactg cgggagcgtg acgtcctgtg actggcagtc gcggaacgtc accgggtcgt
      421 cgccgaccgg tcccacggtc aggaacgaag ccgtgtagca ggtcatgtag aagtagcctc
      481 ccacgaccag gctctcggcg gggcagctct cggcgttcca cgagatgcga ttgctggagt
      541 gggcttcctg gtaccacccg gtgccgggct cctccgagtc ggcgcagaga ttccaggtga
      601 agaggtcgac gccggaaacg gtcacggcgt cgtagtcgat gccgaagctc accgaggcca
      661 ggccctgggc cggatcgata ccggcggcca ccaggtacac gtagtagttg cctgccccgc
      721 tcggggcata gagctgtccc agcacgtcga catcctggca ccggctgagg ttggcctggc
      781 atggctgggc catgctggca cgccttgcat ggaacagcag cttgggatcc gcggcttcgg
      841 cggcgtttgc caggacgccc aggagcaggg cgcccagtgc caggcggatc atcggcccag
      901 caggctcttg atggagctcc agctggtcgg agccaccggc accggcatgc actccgcgac
      961 acagggattg cacccctcgg tgaccgcacc cgccgaaaac gcgacgaagc ccaggtcttt
     1021 cgtgaggagc ggctcggatt cgaccgagct gcagtcggcc agggcggcct tgttgtccac
     1081 cgggcgggtg gtgaggcgca tggtggccgg tgcgtaggcg ccgagataga agtagccggc
     1141 cacgcccact tcgcccgtct ggcaggcatt catcgagtcc caggtgatga ggttgccgcc
     1201 ccccggagcc ggccaggcat ccgcgcccgg cgtgggaaat tccagtgtgg cgcagttcga
     1261 ccaggaggcc acgttgatcg gcacctggcc tccgttggga tcggctccac cttcgtactg
     1321 gatgccgcac tgcacgccgg ctagattcgg aatgcttgcc ttcaccgcca gcaggtagac
     1381 gaagtagaag gtgttcggat cgttgacatt cgtgaggttg gccttgacca cggtggatgc
     1441 ctggcagtcg ttcacgctgc cgaagctgca ggggtttttc gtggtcttgg acttcacatg
     1501 gagcaggagt ttggggcgat tgtcgaacga ggtggccgaa gccggcgcgg cgaaggcaaa
     1561 gccgattccc agagcgatga ttccgacgac gagttggcga caacgcattg gacggggtct
     1621 ccgggatgtg gaggtgaacg gagctggctg gtccggaaca ttgcgggccc ggcgtaccgg
     1681 gcactccggg gcgtgaacgc tacggaacgg catcagaccc gtcaacccaa aagcgaaccc
     1741 cgagggcccg tgaggaccct cggggtttcg gtggttctcc cgatcgtcag gcccggagga
     1801 gtcgggctgc ttgacgattc gtcagtgatt gagtgtcttg atgctgctcc aggtgctcgg
     1861 ctccacgccc acgatcccgc agttctgcac gcagggattg catccgggaa ccgttgcgcc
     1921 ggccgagaaa gaggcccaac ccagctccga agcggccagc ggaatctcga gcgagccgca
     1981 atcggcaacc tttgccgcca tgtcgatcgg gcgggggatg agcttcaacg tggatggtgc
     2041 ataggcggtc aggtagaaat atcccgctgt ggccacggcg cccgtctggc a
//
LOCUS       DASVOD010000390         7115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_235032, whole genome shotgun sequence.
ACCESSION   DASVOD010000390 DASVOD010000000
VERSION     DASVOD010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235032"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VF720_08900"
     CDS             complement(<1..688)
                     /locus_tag="VF720_08900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879515.1"
                     /translation="MTDPLIGESVSHYTVSRKLGSGGMGVVYEAEDKRLGRKVALKFL
                     PAEMARDAASLERFQREARSASALNHPGICTVYDIDQHDGRHFIAMELLEGSTLADAI
                     RKGPLDLRDLLDLGVQLADALESAHAKGIVHRDLKPANIFVNSRGQAKILDFGLAKME
                     RSPKAGADAPSQAATAIHNNELTVAGSTIGTVSYMSPEQARGQVTDARTDLFSLGTVL
                     YQAATGELPFK"
     gene            782..1867
                     /locus_tag="VF720_08905"
     CDS             782..1867
                     /locus_tag="VF720_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7879516.1"
                     /translation="MTNLFFPAVLLATLHLFLIGDAGKPRPGGEPVLEALRHELERDP
                     AASAVVFLGDNIYESGLPEPSDPDYDEMARRIGDQVRTVTELGVRGLFLPGNHDWAGK
                     GQEEWKRVQRQEALVESLGTEFVRWLPDRGCPGPEVIDDWNPGLRLIVLDTQWWLQGN
                     DRPEDAGICPAPNDTAVVAALSNALATAGERRVIVVAHHPLATRGAHGGHFTVLDHLV
                     PLRNLRPWLLIPLPVIGSLYPFGRAHGVYRQDLSASRNKYMRAHLMEAFRSRPPWMYV
                     AGHEHNLQLLDGKAELGPGAPAHLVVSGAGVAGHTEPVDRDRTRVASSKAGFFRLDFN
                     DDGTASLTMFTVSKDGVASEAWRGPVE"
     gene            1951..3633
                     /locus_tag="VF720_08910"
     CDS             1951..3633
                     /locus_tag="VF720_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879517.1"
                     /translation="MRLAAAGTPRGPKVPRRSRARNPRAATILLVLLALLGLSLVIGN
                     AWVAEDAFITFRVVDNAVHGYGFRWNVDERVEVFTNPLWMLIHIPFHALWSNIFLLTI
                     AISLVCTVLAVVIAARAIRGPHAFLAVTLLLPLLLSKTFVEFSTSGLENPMSHLLAAL
                     VFWLLLRHRDAGGRFPWFWLTCVIALSGVNRLDTLLLYLPLTAYLLVTRFREVPWGDV
                     VAGTLPLVAWKLFALFYYGFPFPNTAYAKLATGASRLSYVEQGAWYLLDWLRRDPVSL
                     LVLLAAIVLATGFLLRRRPRIPAPADAGLLASLAVGVLLYGLYVISVGGDFMAGRFWS
                     LCLFVLVLAVAAALPRDLPIRRLAGIALLLLMTAGLSRWTSPVEAFREGRFVRTWRNI
                     DDERLKYAEGHAFYEPRRQHPFQWSMSMETNRPDIIMWYAVGLNAYRCGSRTIVVDGL
                     GLADPLLARMPPLPDRPFYVGHFLRGIPRDYMMARVLHSLDPLQPDLRNYYGKLKLII
                     SGPLFDGERLATLVRFNMGEYDTMKEAYLASGEPYDALVKGDALYRGVAEVP"
     gene            3724..5937
                     /locus_tag="VF720_08915"
     CDS             3724..5937
                     /locus_tag="VF720_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7879518.1"
                     /translation="MNGKVVSHYRVVEQLGSGGMGVVYRAEDTVLDRSVALKFLPAEA
                     IRDKPALDRFLREARSAAALNHPHICTIHEIGEHDGEPFIVMELLTGHTLKQRIDGRA
                     MKVDAILDIAIQIAEALEAAHAKGIVHRDIKPANIFVSENGTAKVLDFGLAKPAAARR
                     DSGSGIDSSQMVTAALGHDLTQPGAAIGTAAYMSPEQARGESLDQRTDLFSFGAIIYE
                     MATGTIAFEGGTSAIVFDQILNGTPRPPAGINPDVPAELERIVGKALEKDPDLRYQTA
                     TEMKADLKRLKRDRDSGSRPSVPRAGSPPVAAETKSKEKSVAVLYFGNLSGAKDDEYF
                     RDGMTEDIITELSKIGQIKVFPRSEVLGFRDKPVTAPEVGQQLDAAFVLEGSIRRAGN
                     RLRITAQLVETRTRHSAWAERYDREMEDVFAIQDEIARSIAQALRVTLSPQEEKIIAK
                     KPTENLQAYDYYLRGRSYTRRENFDFALQMFEQAIKLDPNFAVAHAGLANVCGMIFEL
                     REQNPKWIEKGVAACERAMVLDPRSPEVLVARARISYAQKAYEAAIQQARLAIEQKGD
                     TDGAYNILGRALFASDRLKEAEALITKAIDASGDDYNTYVPYLNVLARLDRRSEAADL
                     RRKMMVVLERQLELVPEDVRARILLAGHCALEGHPEDADRHLQMSVALRPGDSNVLYN
                     AACTYGILGDREQAMATIRKAFEAGYGNHEWASRDPDLTILRDEPEFQKLCKPVG"
     gene            6078..6647
                     /locus_tag="VF720_08920"
     CDS             6078..6647
                     /locus_tag="VF720_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7879519.1"
                     /translation="MTPPRVDDDVIEACRKGDRDALRRIFLDCKDLVYSTAVHYFGGD
                     RTMAADITQDVFVKLAERIGQYRGEARFTTWLQRITVNACLDEKRRTRRLVPLDDEPD
                     VEAITDPTPVPKPEMARAVADALSGLNAQVRMTILLKYVDDLSYDDIAGAMGCSRGTV
                     ASRLNRGHAALARRLAHWKRKLSTGGGHV"
     gene            6640..>7115
                     /locus_tag="VF720_08925"
     CDS             6640..>7115
                     /locus_tag="VF720_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7879520.1"
                     /translation="MFERHVERELSAYCQGELAPRRRREVSDHLAACARCRVAHDEIQ
                     SGIELAGLLPLVEAPAELWQGIEADLGRARARGGARGSPTARTAPVRRGLFEIPVVRW
                     AAAAALLVVIGALVARPLMAPRATSWSVTALAGAPSVGNGTLKGSGRLPVGSWLRT"
BASE COUNT         1144 a         2447 c         2336 g         1188 t
ORIGIN      
        1 ccttgaatgg gagctcgccc gtggctgcct ggtacagcac ggtgccgagc gagaagaggt
       61 cggtgcgcgc gtcggtgacc tggccgcgcg cctgctcggg cgacatgtac gacacggtgc
      121 cgatcgtgct gccggccacc gtgagctcat tgttgtggat ggcggtggcc gcctgcgaag
      181 gggcatccgc gccggccttg ggcgagcgtt ccatcttggc gaggccgaag tcgaggatct
      241 tggcctggcc gcgcgagttc acgaagatgt tggccggctt cagatcccgg tgaacgattc
      301 ccttggcatg cgccgactcc agcgcgtcgg cgagctggac cccgagatcg agcaggtcgc
      361 gcaggtcgag cggccccttc cggatggcat cggccagcgt ggacccctcg agaagctcca
      421 tggcgatgaa gtgccgcccg tcgtgctggt cgatgtcgta gaccgtgcag atgccggggt
      481 gattgagcgc cgaggccgat cgggcctccc gctggaatcg ctcgagcgag gcggcatccc
      541 gcgccatttc ggccggcagg aacttgagag ccaccttgcg gcccaggcgc ttgtcctcgg
      601 cttcgtacac gacccccatc ccgcccgagc cgagcttccg gctcacggtg tagtgggaga
      661 cgctctcgcc gatcaggggg tcggtcattc acaacctttc gttggcatgt gggccggaaa
      721 tcataacccg gacggtcggt gatgggacat tttttccgcc ggaaagggta ccctgccgcg
      781 cgtgacgaac ctgttctttc cggccgtcct gctggccact ctccatctgt tcctgatcgg
      841 cgatgccggc aagccgcgcc ccggcggtga gccggtgctc gaagccctcc ggcacgagct
      901 cgaaagggat cccgcggcca gcgcggtcgt ctttctcggc gacaacatct acgagagcgg
      961 gcttcctgag ccctccgacc cggattacga cgagatggcc cgccgcatcg gcgaccaggt
     1021 ccgcaccgtc acggagctcg gggtgcgcgg cctcttcctt cccggcaacc acgactgggc
     1081 cggcaagggg caggaggagt ggaagcgggt gcagcgccag gaggcgctgg tcgaatcgct
     1141 cgggacggaa ttcgtccggt ggcttccgga tcgggggtgt ccgggccccg aggtgatcga
     1201 cgactggaat cccgggcttc gtctgatcgt gctcgacacg cagtggtggt tgcaggggaa
     1261 cgaccgccct gaggatgccg ggatctgtcc cgccccgaac gacacggcgg tcgtggccgc
     1321 gctctcgaac gcacttgcca cggccggtga gcgccgcgtg atcgtggtgg cgcaccatcc
     1381 gctggccacc cgtggcgcgc atggcggaca cttcacggtc ctcgaccacc tcgtaccgct
     1441 gcggaatctc cgcccgtggc tcctgatccc tcttccggtc atcggctcgc tctatccgtt
     1501 cggccgcgcg cacggcgtct atcgtcagga cctgtcggcg agccggaaca agtacatgcg
     1561 cgcgcacctc atggaggcgt tccgttcccg ccctccctgg atgtacgtgg cgggtcacga
     1621 gcacaacctc cagttgctcg acggcaaggc cgagctcgga cccggcgcgc cggcccacct
     1681 ggtggtgagc ggcgccggcg ttgccggtca cacggaaccg gtcgatcgcg atcggacccg
     1741 ggtggcctca tcgaaggccg gcttcttccg gctcgacttc aacgacgacg ggacagcctc
     1801 cctcacgatg ttcaccgtgt cgaaggatgg cgttgcgagc gaagcctgga gaggaccggt
     1861 cgagtaggac atggcaaggt cctgtttgac atcaatcgaa tctgaataat tcagttttgc
     1921 caaaaacggt tacaaatgta aacttgttgt atgagactgg ccgccgccgg gaccccgagg
     1981 ggcccgaagg tcccccgccg atcgcgcgcc cggaatcccc gggcggccac catcctcctc
     2041 gtcctgcttg ctcttctcgg cctgtcactc gtgatcggaa atgcctgggt ggccgaggac
     2101 gccttcatca ccttccgcgt ggtcgacaat gccgtgcacg gatacggctt ccggtggaac
     2161 gtggacgagc gcgtcgaagt cttcacgaac ccgctctgga tgctgatcca cattcccttc
     2221 cacgccctct ggtcgaacat cttcctgctg acgatcgcaa tctcgctggt ctgcacggtg
     2281 ctggcggttg tcatcgcggc ccgcgcgatc cggggcccgc acgccttcct ggccgtgacg
     2341 ctcctcctgc cgctcctcct ctcgaagacc ttcgtggaat tctccacttc gggcctcgag
     2401 aacccgatgt cgcatctcct ggccgcgctg gtcttctggc tcctcctccg acatcgcgac
     2461 gcgggcggcc gctttccctg gttctggctg acgtgcgtca tcgcactgtc gggcgtcaac
     2521 cgcctcgaca cgctgctcct ctacctgccg ctgacggctt atctcctcgt gacccgcttc
     2581 cgtgaggttc cgtgggggga cgtggtggcc ggcacgctgc cgctcgtcgc gtggaaactc
     2641 ttcgccctct tctactacgg ctttcccttc cccaacacgg cgtacgccaa gctggccacc
     2701 ggcgcctcgc gactcagtta tgttgaacag ggagcgtggt acctcctgga ctggctccgc
     2761 cgggacccgg tgtcgttgct cgtcctcctg gccgcaatcg tgcttgcgac cggttttctg
     2821 cttcggcgca ggccccgcat tccggctcca gccgatgcgg ggctcctcgc gtcgctcgcg
     2881 gtcggtgtcc tgctctacgg cctgtacgtg atctcggtcg gaggcgattt catggcgggg
     2941 cgcttctgga gcctctgcct cttcgtgctc gtcctggccg tggccgccgc cctccccagg
     3001 gacctcccga tccggaggct ggcggggatc gccttgctgc tcctgatgac cgcggggctg
     3061 tcacgctgga cgagtcccgt cgaggcattc cgcgaaggga gattcgtgcg gacctggcgc
     3121 aacatcgacg acgagcggct caagtacgcc gagggacacg ccttttacga accgcgccgg
     3181 cagcacccct tccagtggtc gatgtcgatg gaaaccaaca ggcccgacat catcatgtgg
     3241 tacgcggtcg gtctgaatgc ctatcgatgc gggagtcgca cgatcgtggt ggacggcctg
     3301 gggctggccg acccgctcct ggcccgcatg ccgccgctcc ccgaccggcc cttctacgtg
     3361 gggcacttcc tccgcggcat cccccgggac tacatgatgg cccgcgtgct ccattcgctc
     3421 gacccgctgc agcccgatct ccggaattac tacgggaagc tcaagctgat catatcgggg
     3481 cccctgttcg acggcgaacg tctggccacc ctcgtccggt tcaacatggg ggagtacgac
     3541 accatgaagg aggcctacct ggcctcaggc gagccgtacg acgcgctcgt gaagggagac
     3601 gcgctctacc ggggcgtcgc cgaggtcccg tgaccttgta gaagcgaggc agtaacgcat
     3661 ccgggatgcc cccgccggga ttggttatac tcgaccgctt cgagtcagta tcccggaggc
     3721 aacgtgaacg gcaaggttgt ttcgcactac cgcgtggtcg agcaactggg ctcgggcggc
     3781 atgggagtgg tgtaccgcgc cgaggacacc gtcctcgacc ggtcggtggc actcaagttc
     3841 ctgccggccg aggccatccg cgacaagccg gccctcgacc ggtttctccg ggaggcgcgc
     3901 tcggccgcgg ccctcaatca tccccacatc tgcacgatcc acgagatcgg ggagcacgac
     3961 ggcgagccct tcatcgtgat ggagctcctc accgggcaca ccttgaagca gcggatcgac
     4021 ggccgggcga tgaaggtgga tgccatcctc gacatcgcca tccagatcgc cgaggccctc
     4081 gaggcggcgc atgccaaggg gatcgttcac cgcgacatca agccggccaa catcttcgtg
     4141 tcggagaacg gcacggccaa ggtgctcgac ttcggcctgg ccaagccggc ggcggcccgc
     4201 cgcgactcgg gctccggcat cgactcgtcg cagatggtga cggcggcgtt ggggcacgac
     4261 ctcacgcagc ccggcgcggc catcggcacc gcggcctaca tgtcgcccga acaggcccgc
     4321 ggcgagtcgc tcgaccagcg gaccgacctc ttctccttcg gcgccatcat ctacgagatg
     4381 gccaccggca cgatcgcgtt cgaggggggc acctcggcca tcgtcttcga ccagatcctg
     4441 aacggcaccc cgcgaccgcc ggccggcatc aacccggacg tgccggccga actcgagcgc
     4501 atcgtcggca aggcgctcga gaaggacccg gacctgcgct accagacggc cacggagatg
     4561 aaggccgacc tgaagcgcct gaaacgcgac cgcgattcgg ggagccgccc gtcggtaccg
     4621 cgcgccggca gtccccccgt ggcggccgaa acgaaatcga aggaaaagtc ggtggccgtc
     4681 ctctacttcg gcaacctgag cggcgccaag gacgacgaat acttccgcga cgggatgacc
     4741 gaggacatca tcaccgagct ctcgaagatc gggcagatca aggtgttccc gcgctccgag
     4801 gtgctcggct tccgcgacaa gccggtgacg gcgcccgagg tggggcagca gctcgacgcg
     4861 gccttcgtgc tcgagggaag catccgccgg gccggcaacc ggctgcggat caccgcgcag
     4921 ctcgtggaaa cgcgcacgcg ccactcggcg tgggccgagc gctacgaccg cgagatggag
     4981 gacgtcttcg ccatccagga cgagatcgcc cgcagtatcg cccaggcgct gcgcgtgacc
     5041 ctctcgccgc aggaagagaa gatcatcgcc aagaagccca ccgaaaatct ccaggcctac
     5101 gactactacc tgcgcggccg cagctacacc cgacgcgaga acttcgactt tgccctgcag
     5161 atgttcgagc aggccatcaa gctcgacccg aacttcgccg tggcccacgc gggcctcgcc
     5221 aacgtgtgcg gcatgatctt cgagctgcgc gagcagaacc cgaaatggat cgagaaaggg
     5281 gtggccgcct gcgagcgcgc catggttctc gatccccgat cgccggaggt gctggtggcc
     5341 cgcgcgcgga tttcctacgc gcagaaggcc tacgaagcgg ccatccagca ggcccggctg
     5401 gccatcgagc agaaggggga tacggacggc gcctacaaca tcctggggcg cgcgctcttc
     5461 gcctccgaca ggctcaagga ggccgaggcg ctcatcacga aggccatcga cgcgagcggc
     5521 gacgactaca acacctacgt gccctacctg aacgtgctcg cgcgcctcga ccgcaggtcc
     5581 gaggcggccg acctgcgccg gaagatgatg gtggtgctcg aacgacagct cgagctggtg
     5641 cccgaggacg tgcgggcgcg catcctgctg gcaggccact gtgcgctcga aggtcatccc
     5701 gaggacgccg accgccacct gcagatgtcg gtggcgctcc ggccgggcga ttccaacgtg
     5761 ctgtacaacg cggcctgcac ctacggcatc ctgggcgacc gtgagcaggc catggccacc
     5821 atccggaagg cattcgaagc cggctacggc aaccacgaat gggcctcccg cgaccccgac
     5881 ctgaccatcc tgcgcgacga gccggagttc cagaagctct gcaagccggt cggctgacga
     5941 ctcgttcccg aaacgagtcc cattcccagc aacggtttcc ccggaaaatt ccgggccccg
     6001 caattcaacg ggaagtttcc gcgcccctcc cgcgtccaga aggcaagtga gacgagccgg
     6061 ccacccgaga tccgaacatg acacctcccc gggtcgatga tgacgtgatc gaggcctgcc
     6121 ggaaggggga tcgcgacgcg ctgcgccgga tcttcctgga ctgcaaggac ctcgtctatt
     6181 ccacggccgt gcattatttc ggaggggatc gaaccatggc ggcggacatc acccaggacg
     6241 tcttcgtgaa gctggccgag cggatcgggc agtaccgcgg ggaagcccgg ttcaccacct
     6301 ggctccagcg gatcacggtg aacgcctgcc tcgacgagaa gcggcgcacg cggcggctgg
     6361 tgccgctcga cgacgagccg gatgtggaag cgatcacgga cccgacgccg gtgccaaagc
     6421 ccgagatggc ccgggccgtt gccgatgccc tctcgggctt gaatgcccag gtgcgcatga
     6481 cgatccttct caagtacgtc gacgatcttt cctatgacga catcgccggc gccatggggt
     6541 gttcgcgcgg caccgtggcc tcgcggctga atcgcggcca tgcggccctg gcccggcgac
     6601 tggcccactg gaagagaaaa ctctcgaccg gaggcggcca tgtttgagcg gcatgtcgaa
     6661 cgcgagctct cggcgtactg ccagggagag ctcgccccac ggcgccggcg cgaggtgtcc
     6721 gaccacctgg ccgcctgcgc gcgttgccgg gtggcccacg atgaaatcca gtccggcatc
     6781 gagctggccg gacttctgcc gctggtggaa gcgccggcgg agttgtggca ggggatcgag
     6841 gccgacctgg gtcgggcgcg tgcccgcggg ggagcgcgtg gatcacctac cgcccgtacc
     6901 gcccccgtcc gccgtgggct gttcgagatt ccggtcgtgc ggtgggccgc cgccgcggcg
     6961 cttctcgtgg tcatcggtgc cctggtggcc cggccgctca tggcgccacg ggcaacctcc
     7021 tggtcggtga ccgcgctggc cggcgcccca agcgtcggca acggaacctt gaaggggtcc
     7081 gggcgccttc cggtcggatc gtggttgcgc accga
//
LOCUS       DASVOD010000391         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_390755, whole genome shotgun sequence.
ACCESSION   DASVOD010000391 DASVOD010000000
VERSION     DASVOD010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390755"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..995
                     /locus_tag="VF720_08930"
     CDS             282..995
                     /locus_tag="VF720_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7879521.1"
                     /translation="MVRCLPAWPEKLIQLAHALQGPDGVLARNEFWYLLNLALLQHLR
                     FQGPRLGLTDDDRLHDLASEKASNLVGKFDRGSWRPQDSSAGELVNFVKTIARNALVD
                     EHRRPRPREVALEEHMADSGVAPRAVEQPIDTIGRDRFVDRLIRCAGNLKPEHRRIWL
                     LRVLYDMPSRAIAAHPDVQLTPGNVDVILARAREQIRNGMGVDPGARHQLPQGTLSAL
                     WEHYRQPADDPGRVGDGNE"
     gene            988..1683
                     /locus_tag="VF720_08935"
     CDS             988..1683
                     /locus_tag="VF720_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879522.1"
                     /translation="MSDQLHLDVDTIIDLAAGLVTGSERRRLLSHIRSCGECERRLMT
                     ALADDELSRMPARAMVPVSGVPGASASRIRLFRIAVPLLLAAATLLIVIGRIGSGPAA
                     PPVSWWMPATADLLQLRDVNSPESDSAFWEGLDAYQRHDVPAAMAAFERSRATGAFDD
                     LRRLYMASLELHTGRPQGALSRLESLQVAYLPEPWRGSARMIEYLALKALGRDAGPPP
                     DSVDALVGRRLPR"
     gene            complement(1720..>3533)
                     /locus_tag="VF720_08940"
     CDS             complement(1720..>3533)
                     /locus_tag="VF720_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879523.1"
                     /translation="SGTWEALLDQVQTLVLAGEFINGEEDVFVDNIRLSTPPAPLAFP
                     CVVERFDAALLLDMRFDNTGGTSLPATGGNLARFCQITDATGTSCVFLPPRFLGDWRA
                     LEGNSTIGLDVRILSYTGTPVGQTEFIRISGPGGAARLSLAAADLPPSARVWKRYAYP
                     IDAAAWTVTSGTWAGLLANVTECRVAPEFVNGTEVVGIDNIFRGSAACGDPDNAVVPR
                     APDVTLCGYDSFIGIASVARQPTSGELFGVVDQDPTPGGGLYRALGPTAGVRLQSYTS
                     PNDLLADGAGNFYVTESISGNVYRYVPNVGSSLWVAGFHSGDDDPFGMCLAPTGFSGP
                     NVTPGDILVTDRGVNGPDEVWAFSTGAAENERLVLPDPGETDYLDLAGTSAGIVYMVD
                     ALDPNRLTRLAPNGTLSYLALSTPVPTPTGIAWDSGDDKLYVVSGSGHSLHRVDPATG
                     TVTLVADGFAGPAPCAVEIDVTARRLWVADKSGGRIYTFCLLDPSGVGDEPTGPAPIA
                     SHLLGGPTVSPNPSPGPASIEFRLGEPATVELAIFDVTGRRVRQLAAAAYDGGAHRVT
                     WDGRDDGGRDVVDGLYFVRAAANREWVTARVTLIRGR"
BASE COUNT          584 a         1224 c         1189 g          536 t
ORIGIN      
        1 gtggtcccga ggagggccag cgcctcatcg tactctccaa gaactcggag agaatccacg
       61 gaccggaagg caacgaggag tggctgggtg cggggtggac ccatggagca gcccggttgg
      121 agtgaggcca cgaggacaag cagggcgggc cattgccatg gggagcaccg tgcggcggcg
      181 atcgtgctca tgacgtggat gagtatactg gtgaggatcg gcaaacgggt gttatcgggc
      241 cactttcacc atcccgaacc ggagaggagc cgctactccc catggttcgt tgcctgccgg
      301 catggccgga aaaactcatc cagctggctc atgccctcca gggccccgac ggggtcctgg
      361 cgcggaatga gttctggtac ctgctcaacc tcgccctgct tcagcacttg cggttccagg
      421 gcccccgcct cggcctcacc gacgatgacc gcctccacga tctggcctcg gaaaaagcgt
      481 caaatctcgt ggggaaattc gatcgtggtt catggagacc gcaggactcg agcgccggag
      541 aactggtgaa tttcgtgaaa acaatcgccc gaaacgccct cgtggacgaa caccgtcgtc
      601 cccgcccgcg ggaggtggcc ctggaggagc acatggccga ttccggcgtg gccccgcgag
      661 ccgtggaaca gccgatcgat accatcggcc gcgaccgctt cgtcgatcgc ctgattcgat
      721 gcgccggcaa cctcaagccg gagcatcgac ggatctggtt gcttcgggtc ctctacgaca
      781 tgccaagccg cgccattgcc gctcatcccg acgtgcaact gacccccggc aacgtggatg
      841 tcatcctggc ccgggcgcgc gagcagattc gcaacgggat gggtgtcgat cccggcgcaa
      901 ggcaccagtt gccgcaggga acgttgtcgg ccctgtggga gcactatcgg cagccggccg
      961 acgatcccgg tcgcgttgga gatggaaatg agtgatcaac tccatctcga cgtcgacacc
     1021 atcatcgacc ttgccgccgg cctggtgacc ggcagcgagc gacgacggct cctttcccac
     1081 atcaggtcat gcggcgaatg cgaacgccgg ctcatgaccg ccctggccga cgacgaactg
     1141 tcacgaatgc cggcccgggc catggtaccg gtatccggcg tacccggcgc ctcggcatcc
     1201 cgaatacgat tgttccggat cgccgtaccc ctgttgctcg cggcggccac gctcctcatc
     1261 gtgatcggcc gcatcggatc cggccccgcc gcgccaccgg tctcgtggtg gatgccggcc
     1321 acggccgacc tcctccagct gcgcgacgtc aattcccccg aatccgattc cgccttctgg
     1381 gaagggcttg acgcctacca gcggcatgat gtgccggccg cgatggccgc cttcgaacga
     1441 tcacgagcca ccggcgcctt cgacgacctg cgccgcctct acatggccag cctcgaactc
     1501 cacaccggcc gacctcaagg cgcactctcc cggctcgagt cgctccaggt ggcctacctc
     1561 cccgaaccct ggcgcggcag cgcgcgcatg atcgagtacc tggcgctcaa ggcgctgggg
     1621 cgcgacgcgg ggccgccgcc ggacagcgtt gatgcgttgg tcgggcgccg cctgccccga
     1681 taacagccga cccatcccca ggtcctgcca tcgcccgagt cagcgccccc ggatcagcgt
     1741 gacgcgcgcc gtgacccact cccggttcgc ggccgccctc acgaagtaga gcccatccac
     1801 gacatcccgc cccccgtcat cccgcccgtc ccacgtgacg cgatgcgcac cgccgtcgta
     1861 cgcggccgcg gccagttgcc tcacgcgccg gccggtcacg tcgaagatgg ccagttcgac
     1921 ggtggccggc tcgccgagtc ggaactcgat cgatgccgga ccgggcgagg ggttcggcga
     1981 gacggtcggc cccccgagga ggtgcgatgc gatcggcgcc gggccggtcg gctcatcgcc
     2041 tactcccgaa ggatcgagca ggcagaaggt gtagatgcgg ccgccgctct tgtcggccac
     2101 ccagaggcgg cgggcggtca cgtcgatctc cacggcgcac ggggccggcc ccgcgaaacc
     2161 gtcggcaacc agcgtcaccg tgccggtggc ggggtcgacc cggtgaagcg agtggccgct
     2221 gccgctcacg acgtacagct tgtcgtcgcc agagtcccag gcgatgccgg tcggcgtcgg
     2281 caccggcgtc gagagtgcca ggtaactgag cgtgccgttc ggagccagcc gcgtgaggcg
     2341 attggggtcg agcgcatcca ccatgtagac gatgccggca gacgtacccg ccaggtcgag
     2401 gtagtcggtt tcaccgggat cgggaaggac gagccgctcg ttctcggccg cgcccgtcga
     2461 aaaggcccac acttcgtcgg ggccgttcac gccgcgatcg gtgacgagga tgtctccggg
     2521 cgtcacgttg ggtccgctga atccggtcgg cgccaggcac atcccgaacg ggtcgtcatc
     2581 gcccgagtgg aagccggcca cccagaggct cgagcccacg ttgggcacgt agcgataaac
     2641 attcccggag atgctttccg taacgtagaa gttgccggcc ccgtcggcca gcaggtcgtt
     2701 gggagacgtg tacgactgca gccgcacgcc ggcggtgggc ccgagggcgc ggtagaggcc
     2761 gcctcccggc gtgggatcct ggtccaccac gccgaacagc tcgccgctcg tcggctgtcg
     2821 cgccacgctc gcgatcccga tgaagctgtc gtagccgcag agcgtgacgt cgggcgcccg
     2881 gggcacgacc gcattgtccg ggtcgccgca ggcggccgag ccgcggaaga tgttgtcgat
     2941 tccgacgacc tcggttccgt tcacgaattc cggagccacg cggcactccg tgacattggc
     3001 caggagcccc gcccaggtgc cggaggtgac agtccaggcc gccgcgtcga tcggatacgc
     3061 gtagcgcttc cagacccgtg cgctcggagg aagatccgcg gcggccagcg aaagccgcgc
     3121 ggcgcccccc gggcccgaga tccggatgaa ctcggtttgc ccgaccggcg tgcccgtgta
     3181 gctcaggatc cggacatcga gcccgatggt gctgttcccc tcgagcgccc gccaatcgcc
     3241 caggaagcgc ggggggagaa agacgcagga ggtgcccgtg gcgtcggtga tctggcagaa
     3301 ccgggccagg ttgccgccgg tggcgggcag gctcgtcccg ccggtgttgt cgaagcgcat
     3361 gtcgaggagg agcgcggcgt cgaagcgctc caccacgcat gggaaagcca gtggcgccgg
     3421 cggggtggag agccggatgt tgtcgacgaa gacgtcctcc tcgccgttga tgaactcgcc
     3481 ggccagcacc agtgtctgca cctgatcgag gagcgcctcc caggtgccgc tca
//
LOCUS       DASVOD010000392         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_287893, whole genome shotgun sequence.
ACCESSION   DASVOD010000392 DASVOD010000000
VERSION     DASVOD010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287893"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..>3519
                     /locus_tag="VF720_08945"
     CDS             288..>3519
                     /locus_tag="VF720_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879524.1"
                     /translation="MPPSRWPFRYCLLLALTSLVSSALLAPPATARPEPEFPYRLIDL
                     AADADPDAPLPAGSGRSGPGLLAGQDTLYFGGTWWAADSMRWEALRDSQWTFETGVGS
                     SINTGFNPNKPIGFHQTMEGWHGSDQTLNPVPYFRRSSTCAISGSFSLWAGVTTAEGN
                     DLCFASGQGYGNSWSMTVSRTFAYPGSGNVTLSWKFAVESEPGFDYANVFIDTTGTGA
                     APDILLSSYDGTTSGTGAALLIPGVRMRTGAGPVRVSFNVDADGGYSDEDGAYPTTCG
                     LAVFDDITLSGAITDVTNFESGMNGWAQEIPTTGIGDFSHLDYIPDLPPVMNQFCPCA
                     PTDSVLVFYDPDGFQPLDMDNIAVCPWLDLRRQGMANRPGKLIQYDYAADLPLTNYIF
                     IQLRGRYYPYVCPGSGTVRVSPWIDPNVFYFGESPVCNPVGVRRNRDYSAIFTASSEQ
                     IQMGIGIINLCRTSPFGPMCTGLTNTTPRFDNIRFGLYGDLNVPVVNTTTFDRYQDNF
                     AADGTLNPASPGRLDQNRIKGASTPESGSILGDTLNARGNGGNTEVRLVFKVRPGPFV
                     NGATLAGMVARWTYEPTLNGQYGTDGNPGTWPNWYSVRMDTAEQGGVPSTSPFTWMST
                     FHESDPGFQGNDRMADPNDPGRLANDILPDHLFTPGSRIDYFVASRYRPPDPRNPTGM
                     NWYTDPDTARAGFREVEILPSSMAADTTWNCVLYVDHHDDRNQDDQYYEELGLKSVLG
                     PGGANAEGTRYDRFDNQTPSSAQMSFGRPLRSKYGCSQTQAFAYRAIAWHGATLSSGV
                     VSREDVAILGPWLNLAVGESGGQRRFWGSGEGIMQSMHNDGSTTRAFMNNTLGVTQSC
                     TGIRLANCPSGTAIDTTYCIPTAAVAGSHFASSIVPRARGNGCPTLLAFDVLGVTPAV
                     TSARGQLDYIKSGLPRSYASVTNLSTGPSGYRSVLDGVSVGRMRSNAGYHLVGCNDIS
                     ASLARTADVFGWFGTLALCRLPADIVAVPGEDSPAAAIPVFASLGLARPNPMVRVTSI
                     SLTQGDVRGPVRLEVFDVTGRLVRRVFDGPLDPGPHEFAWDGRAADG"
BASE COUNT          578 a         1194 c         1125 g          622 t
ORIGIN      
        1 ggtgggggcg gggggacggt aggttccgag cgcccccgtc ggaccccggc accctggctg
       61 gcgatcaata ctgtttcagc attagcttaa gtcattcagc caaatcaact tcgagttggt
      121 ctcaactcgg tccgaacata gttcatccgc caccccgcgt tctgacacat gacccgggcg
      181 cggagacaaa accgtccgta tattctctcc cagagagcct cccggggcgc ggcggtccat
      241 ctgcccggac gccgcgcccc aattcgcgcc accgaccgga gaaccccatg ccaccctccc
      301 gctggccgtt ccggtattgc ctgctgttgg ccctgacgtc gctggtctcc tccgcactgc
      361 tcgccccacc cgccaccgcc cgccccgaac ccgaatttcc ctaccggctg atcgacctgg
      421 cggccgacgc cgatccggac gccccgttac ccgccgggtc gggacggtcc ggccccggac
      481 tgctcgccgg ccaggacacc ctctatttcg gcgggacctg gtgggccgcc gactcgatgc
      541 ggtgggaggc gctgcgtgac agccagtgga cgttcgagac cggcgtcggc tcgtcgatca
      601 acacgggctt caacccgaac aagccgatcg gctttcacca gacgatggag ggttggcacg
      661 gctccgacca gacgctgaac ccggtgccgt actttcggcg ctcctcgacc tgcgcgatct
      721 cgggatcgtt ctcgctctgg gccggtgtga cgacggccga ggggaacgat ctctgcttcg
      781 ccagcggaca ggggtacggg aacagctggt cgatgaccgt ctccaggacg tttgcctatc
      841 ccggcagcgg caacgtgacg ctgagctgga agttcgccgt cgagtcggag ccggggttcg
      901 actacgccaa cgtgttcatc gatacgaccg ggaccggcgc ggctccggac atcctccttt
      961 cgagctacga cggcacgacg agcgggaccg gtgcggcgct gctcatcccc ggggtgcgga
     1021 tgcggaccgg agcggggcct gtgcgggtat cgttcaacgt cgatgctgat ggcggttact
     1081 ccgacgagga cggcgcctac ccgaccacct gcggccttgc cgtcttcgac gacatcacgc
     1141 tgagcggggc gatcaccgac gtcacgaact tcgaaagcgg gatgaacggg tgggcacagg
     1201 aaataccgac gaccggcatc ggggactttt cgcacctgga ctacatcccg gacctgccgc
     1261 cggtcatgaa ccagttctgt ccgtgcgccc cgaccgattc ggtgctcgtg ttctacgacc
     1321 cggacgggtt ccagcccctc gacatggaca acatcgccgt ctgtccctgg ctcgacctgc
     1381 gtcgacaggg gatggcgaat cgaccgggca agctcatcca gtacgactat gcggccgatt
     1441 tgcctctcac gaattacatc ttcattcagt tgcgcggccg gtattatccc tacgtgtgtc
     1501 ctggtagcgg gacggtgcgc gtgtcgccct ggatcgatcc caacgtcttc tatttcggtg
     1561 agtcgcccgt ctgcaatccc gttggcgtaa ggcgcaatcg cgactactcg gcgatcttca
     1621 cggcgtcgtc cgagcagatc cagatgggga tcggcatcat caatctctgc cggacctcgc
     1681 cgttcggccc catgtgcacc gggctcacca acacgacgcc ccggttcgac aacatccggt
     1741 tcgggctcta cggcgatttg aatgtcccgg tcgtcaacac caccaccttc gaccgttacc
     1801 aggacaactt cgccgctgac ggaacgctga accccgcctc gccggggcgg ctggaccaga
     1861 atcggatcaa gggggcgtcc acgccggagt ccggctcgat cctcggcgac acgctcaatg
     1921 cccgcggcaa cggcggcaat accgaagtgc gcctcgtctt caaggtgcgt cccggcccgt
     1981 tcgtgaacgg cgccacgctg gcgggtatgg tggcccgctg gacgtacgag ccgaccctga
     2041 atggtcaata cggtaccgac ggcaacccgg ggacctggcc caactggtac tcggtcagga
     2101 tggacacggc ggagcagggg ggcgtgccct ccacgtcacc gttcacctgg atgtccacct
     2161 tccacgagtc ggatcccggc ttccagggaa atgaccgcat ggctgatcca aacgaccccg
     2221 ggcggctcgc caatgacatc ctgccagacc acctcttcac accgggttcg cgcatcgact
     2281 acttcgtggc ctcgcgctat cggccgcccg atccccgaaa tcccaccgga atgaactggt
     2341 acaccgatcc cgacaccgcg cgggccgggt tccgggaggt ggagatcctt ccgtcgtcga
     2401 tggcggcgga cacgacgtgg aactgtgtcc tgtatgtcga tcaccacgac gatcggaacc
     2461 aggacgacca gtactacgaa gagcttggac tcaagtcggt gctcgggcct ggcggcgcca
     2521 atgccgaagg gacgcgttac gatcgcttcg acaaccagac gccgtcctcg gcgcagatgt
     2581 cgttcggccg gccactgagg tcgaaatacg gatgcagcca gacgcaggcg ttcgcgtacc
     2641 gggcgatcgc ctggcacggg gcgacgctga gcagcggtgt cgtgagtcgt gaagatgtcg
     2701 cgatcctcgg cccatggctg aacctggccg tgggcgagtc gggcgggcag cgccgcttct
     2761 ggggttcggg agaggggatc atgcagtcga tgcacaacga cgggagcacg acccgggcct
     2821 tcatgaacaa tacgctgggc gtgacgcagt cgtgcaccgg gatccggctt gcgaattgcc
     2881 cgagcggcac cgccatcgac acgacctact gcatcccgac cgcggccgtg gccggttccc
     2941 acttcgcgtc gtccatcgtg ccccgggccc gcggaaacgg ctgcccgacg ttgctggcgt
     3001 ttgacgtgct gggcgtcacg cccgcggtga cgtcggcgcg cgggcagctc gactacatca
     3061 agtcggggct gccgaggagt tacgcgtcgg tcaccaacct gtcgacaggt ccctctggat
     3121 accggagcgt actcgacgga gtttccgtcg gccggatgcg ctcgaacgcc ggttaccact
     3181 tggtcggctg taatgacatc agtgcctcgc tggcccgcac cgccgatgtt ttcggctggt
     3241 tcggcacgct ggcgttgtgc cggcttcccg cggacatcgt cgccgtgccc ggtgaggatt
     3301 cgcccgcggc ggcaatacct gtattcgctt cactcggctt ggcgcggccg aacccgatgg
     3361 tgcgggtcac ttcgatctcg ctgacgcagg gcgatgtgcg cggcccggtg cgcctcgagg
     3421 tgttcgacgt caccgggcgg ttggtacgcc gtgtcttcga cggtccgctc gatccgggac
     3481 cgcacgagtt cgcctgggac ggccgcgcgg ccgacgggc
//
LOCUS       DASVOD010000393         6855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_183766, whole genome shotgun sequence.
ACCESSION   DASVOD010000393 DASVOD010000000
VERSION     DASVOD010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6855
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183766"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VF720_08950"
     CDS             complement(<1..315)
                     /locus_tag="VF720_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879525.1"
                     /translation="MLRPTRALPAAVLSAVLVAGGVVAAWMTRAADAPPSAPVEPHLK
                     NIRQLTSGGENAEAYFSFDGRQLTLQSSRPPYECDQIFVLDLAPKDPSSPLYSRLVST
                     GKG"
     gene            complement(315..1436)
                     /locus_tag="VF720_08955"
     CDS             complement(315..1436)
                     /locus_tag="VF720_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEX7879526.1"
                     /translation="MRLISTFDDWIDLFRTWRDKLGIDKELMKEYQFEHLFGGVHSDE
                     IEFGAYAGARKWEKVIEIPDQRVRDALIHLIVYQGDTEFASVEQQKLLLNTAPNTYDL
                     MSMARVMREEMRHGWQMSYLLVKYFGTTGRVEAQKLLSRNADNFERLLNTFNEPIEHW
                     LDFFAFADFIDRDGKFQLKMLSHSAFAPLARSMPPMLREESFHLGTGNNGLRRIVQAK
                     RVPNAIVQKYLNKWISTAYDLFGSSHSSSAHWAYVWGLKGRFDEEVNQNEADLDQLNE
                     YNRSLYHAEVADIVSRFNKMLGPDDVPFTTPDVKFHRAIGEYAGKPYSVTGELLSPEA
                     YARHLDEVMPNDRDQLVLKDIFKTNDWIIAREYTKESLN"
     gene            complement(1613..3508)
                     /gene="asnB"
                     /locus_tag="VF720_08960"
     CDS             complement(1613..3508)
                     /gene="asnB"
                     /locus_tag="VF720_08960"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7879527.1"
                     /translation="MCGITGFLRLDASGGSSVGASVSDRDRPCLRAMTDACAHRGPDG
                     EGFFDDGICALGHRRLAIIDLSTGDQPMSNSRGDCTIVFNGEIYNYVELKAELEGRGH
                     KFRTQSDTEVILAAYDEWGDACQSRFNGMWAFALWDRRRRRLLISRDRLGEKPLHYAV
                     HGGRLWFASTIRSLIAGGVPPEKCLDLVEVYLVLGYVPGPQTFYKHIRRLPAGNSLVV
                     EGNSNPQVSAWWDLPAVPEAAMLTDRKHVDERFEELFDDSVRIRMRSDVPFGAFLSGG
                     LDSASIVSSMARVSKEPVRTFTIGFDDKEFDERELARLVAERFGTRHREEVVQPETLA
                     HALKLVARHTDQPFGDSSALPTEQVSRVARHHVTMVLTGDGGDEVLSGYTSYQGEKFA
                     AQFQGLPSPLRMAAPALARGVGQLTGGGLRYKMNRVRNVLESSGQPYRERLISKMAWA
                     PLPQVKALLAPAPARVSIEEYLDDFMGRCPWKDPFYRHMYFHLKLSLPDDMLLKVDRM
                     SMAHGLETRVPFLDFRLVELMIGVHRDVKMNGYERKGVLRRSVGRRLPPAVLTAPKKG
                     FAVPLRSWFQKESARPFLDDMQSGLSGLVDRKVLDQVVRENSSGAEDRGNFLWMLTIL
                     SGWLSVV"
     gene            3630..5858
                     /locus_tag="VF720_08965"
     CDS             3630..5858
                     /locus_tag="VF720_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009102160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HEX7879528.1"
                     /translation="MTAGGGWAALLYSLERAGDAGGLWKLYRRLSRKNACKTCAFGMG
                     GQQGGMTNESGAWPEVCKKSIQAQAADMQPPIPLSLFERTSVGHFATMDPMTLEYLGR
                     IGHPMVLRSGDTHYRPIAWEDALDAIAADFRERSPDALTFYSSGRASNEAAFLLQCVA
                     RAAGTNNVHNCSYYCHQASGVGLHQALGSGTASVSLDDLEHADLAMVIGANPASNHPR
                     LITQLVAMRKRGGQVIVLNPVRELGLERFRIPSQPWSFVFGSKVSDLYLQPKIGGDIA
                     VLKGMLKALLAREGAIDRSFLASHVDGWPAVESDAMATSWETIERHSGLTRAAVEDAA
                     ARYANSKKALFMWAMGITHHEHGVDNVFAITNLALARGMVGKPHAGVMPIRGHSNVQG
                     IGSVGVAPALKEAFAERLTRLYGIEVPKTPGLDTYRTILAMNDGTITGAFCLGGNLFS
                     SNPDTDFTRTAMQKVETTVYVSTKLNPGHFHGRGRTTYILPCLARDEEHQATSQESMF
                     NYVRLSEGGSEPATADMKSEVEIIAGLAARWLPKGKVDWSRFTSHDALRAAIADVVPG
                     WEDLRNMGTTKREFTISGRVRHVPEFPLPGGKARAFVTPMPDNLPPPGHLMLMTLRSE
                     GQFNTVVYDKEDIYRAIDRRDVAMLNADDAMRMGFAHDDIVRVETTAGALDGLRVRIL
                     DVPAGSLVMYYPEANAIVPRKIDPKSGTPAFKSVPARVVKSVGRPAPHHARKASESVV
                     PV"
     gene            complement(6073..>6855)
                     /locus_tag="VF720_08970"
     CDS             complement(6073..>6855)
                     /locus_tag="VF720_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX7879529.1"
                     /translation="VYPALGMGSNSSISMNGDGTFGGSSREGRHQVKVSGSGSGKEMW
                     ADLRIEMPKGADLKLYNAIGKVVVSEVSGELNLDTASGDVEATGVTGSVVVDCGSGDV
                     ELTGIDGTVSVDTGSGNVILSDVKGDMVDADTGSGDIEGDHLSCDQLSFDTGSGDINI
                     ESVSVSKISCDTGSGSILVAIQGDLDGGNFDTGSGDVTLEVGPDLGASLDIETGSGDI
                     DIDAPHQATRNRRSEFRGTVGDGKGNVIIETGSGDVRVTSAR"
BASE COUNT         1189 a         2226 c         2259 g         1181 t
ORIGIN      
        1 ccccttcccg gtcgacacca gccggctgta caggggcgat gatggatcct tcggggccag
       61 gtcgagcacg aagatctggt cgcactcgta cggcggccgg ctggactgga gcgtgagctg
      121 gcgaccatcg aacgagaagt aggcctcggc gttctcgccg ccggacgtga gctggcggat
      181 gttcttgagg tgcggctcga ccggcgcgga cgggggcgca tcggcggcgc gcgtcatcca
      241 tgccgccacg acgccgcccg ccaccaggac cgccgacagg acggcggcgg gaagggcccg
      301 ggtcgggcga agcatcaatt caacgattcc ttggtgtact cgcgcgcgat gatccagtcg
      361 ttcgtcttga agatgtcctt caacacgagc tggtcacggt cgttgggcat cacctcgtcg
      421 aggtgcctgg cgtacgcctc gggcgagagg agctcgccgg tgacgctgta cggcttgccg
      481 gcgtactcgc cgatggcgcg gtggaacttg acgtcgggcg tggtgaacgg cacatcatcg
      541 ggcccgagca tcttgttgaa gcggctcacg atgtccgcca cctcggcgtg atacaggctg
      601 cggttgtact cgttcaactg atcgaggtcg gcctcgttct ggttgacctc ctcgtcgaac
      661 cgccccttga gcccccacac gtacgcccag tgggcgctcg aggagtgcga gctgccgaac
      721 aggtcgtagg cggtggagat ccacttgttc aggtacttct gcacgatggc gttcggcacc
      781 ctcttggcct gcacgatccg gcgcaggccg ttgttgcccg tgccaaggtg gaacgactcc
      841 tcgcgcagca tcggcggcat gctccgggcc agcggcgcaa acgccgagtg cgaaagcatc
      901 ttgagctgga acttgccgtc ccggtcgatg aaatcggcaa aggcaaagaa gtcgagccag
      961 tgctcgatcg gctcgttgaa cgtattgagc agccgttcga agttgtcggc attgcgggag
     1021 agaagcttct gggcctccac gcgcccggtg gtgccgaagt acttcacgag gaggtacgac
     1081 atctgccacc cgtgccgcat ctcctcgcgc atgacacggg ccatcgacat caggtcgtag
     1141 gtgttgggcg ccgtgttcag gaggagcttc tgctgctcca cggaggcaaa ttccgtgtct
     1201 ccctggtaca cgatcaggtg gatgagggca tcgcgcaccc gctggtcggg gatctcgatc
     1261 accttttccc acttgcgcgc cccggcatag gccccgaact cgatctcgtc cgaatgcacc
     1321 cccccgaaga gatgctcgaa ctggtattcc ttcatcagct ccttgtcgat gccgagcttg
     1381 tcacgccagg tgcgaaagag gtcgatccag tcgtcgaagg tcgagatgag ccgcatgcgg
     1441 ggagtgcctc ctgtgcgtgg ccgatttcag atcgagcgca ttcagccgcg gattttattt
     1501 tattgcaatg gcttacgagt ccattccgtc cttcgacggg gccgaaagga tacacgagag
     1561 gggcacgccg gttcaattgt tcgacactgg acgccagact ctgaaacact gatcacacca
     1621 ccgacagcca cccactgagg attgtcagca tccagaggaa attgccgcgg tcctcggcgc
     1681 ccgaagagtt ctcgcgaacc acctggtcca gcaccttccg gtcgacgagg ccactcaaac
     1741 cggactgcat gtcatccagg aacggccggg ccgattcctt ctggaaccag ctgcggagcg
     1801 gaacggcgaa acccttcttc ggcgccgtca gcacggcggg ggggaggcgg cgacccaccg
     1861 accggcgcag cacgcccttc cgttcgtagc cgttcatctt cacgtcgcgg tggacgccga
     1921 tcatcagctc caccaggcgg aagtcgagga acggcacgcg cgtctcgagg ccgtgcgcca
     1981 tgctcatccg gtccaccttc aacagcatgt cgtcggggag gctcagcttc aggtggaagt
     2041 acatgtggcg gtagaaagga tccttccagg gacagcgccc catgaagtcg tcgaggtact
     2101 cctcgatcga gacgcgggct ggcgccgggg caagaagggc cttcacctgg ggtagaggcg
     2161 cccaggccat cttgctgatg aggcgttcgc ggtagggctg cccgctcgac tccagcacat
     2221 tgcgcacccg attcatcttg tagcgcaggc ccccgccggt gagttgtccg acgccacggg
     2281 caagtgcggg tgcggccatg cggagcggtg acggaaggcc ctggaattgc gcggcgaatt
     2341 tctctccctg gtagctggtg tagccggaga ggacctcgtc gccgccgtcg ccggtgagca
     2401 ccatggtcac atggtgacgg gccacgcgac tcacctgctc ggtggggagc gccgaggaat
     2461 cgccgaacgg ctggtcggta tggcgcgcca cgagcttgag ggcatgcgcg agcgtttcgg
     2521 gctgcaccac ttcttcccgg tgacgggtgc cgaagcgctc ggccaccagg cgggccagct
     2581 cgcgctcgtc gaactccttg tcgtcgaaac cgatggtgaa ggtgcgcacc ggttccttcg
     2641 acacgcgggc catgctcgac acgatgctgg ccgagtcgag gccgcccgac agaaaggcgc
     2701 cgaacggcac gtcgctgcgc atgcggatac ggaccgagtc gtcgaagagt tcctcgaagc
     2761 gctcgtccac gtgcttccgg tcggtcagca tggccgcttc cggcacggcc ggcaggtccc
     2821 accaggccga tacctgggga ttcgaattcc cctcgacaac caggctgttg cccgccggca
     2881 accggcggat atgtttgtag aacgtttgcg gccccggtac gtagccgagc acgaggtaca
     2941 cctcgaccag gtcgaggcac ttctccggcg gcactccccc ggcgatcagg ctccggatcg
     3001 tggaggcaaa ccagagtcgt ccgccgtgca ccgcgtagtg gagcggcttc tcgccgagac
     3061 ggtcgcgcga gatgaggagg cggcggcggc ggcgatccca gagcgcgaag gcccacatgc
     3121 cgttgaagcg cgactggcag gcatcgcccc actcgtcgta cgcggccagt atgacctcgg
     3181 tatccgactg cgtgcggaac ttgtggccgc gcccttcgag ctcggccttg agctccacgt
     3241 agttgtagat ctcgccgttg aagacgatgg tgcagtcgcc gcggctgttc gacatcggct
     3301 ggtcgccggt cgagaggtcg atgatggcga ggcgccgatg tccgagcgcg cagatgccgt
     3361 cgtcgaagaa accttcgccg tcggggccgc gatgcgcgca ggcgtcagtc atggcgcgga
     3421 ggcacggtcg gtcgcgatcg gaaacggaag cgccaacgga cgaacccccg ctagcatcaa
     3481 ggcggagaaa tcccgttatc ccgcacatgg gaaatcgttc gatggagggg gaagcggcct
     3541 gcaggcgagc acctatccat tatatgcgga aaacgggtga tcgcttgccc gctttcgaca
     3601 acaaacccat actgttcgcg atgaaacgaa tgactgccgg cggtggatgg gccgccctgc
     3661 tctactcgct cgagagagcc ggcgatgccg ggggactctg gaagctctac cggcgcctct
     3721 cccggaagaa tgcctgcaag acgtgcgcat tcggcatggg aggccagcaa ggtggcatga
     3781 cgaacgagtc gggcgcctgg ccggaggtgt gcaagaagtc gatccaggcg caggcggccg
     3841 atatgcagcc tcctattccc ttgtcactct tcgagcgcac cagtgtgggg cattttgcca
     3901 cgatggaccc gatgaccctg gagtacctgg ggcggatcgg tcatccgatg gttcttcgtt
     3961 ccggcgacac ccactaccgc ccgatcgcct gggaagacgc gctcgacgcc atcgcggccg
     4021 acttccgcga gcgcagcccc gacgcactca ccttctattc gtcgggccgg gccagcaacg
     4081 aggcggcctt cctgctgcag tgcgtggcgc gggccgccgg cacgaacaac gtccacaact
     4141 gctcgtacta ctgccaccag gcctcggggg tcggattgca ccaggcgctc ggctcgggca
     4201 ccgcctcggt gagcctcgac gatctcgagc acgccgacct cgccatggtg atcggcgcca
     4261 acccggcctc gaaccatccc cgcctcatca cgcagctggt ggcgatgcgc aagcgcggcg
     4321 gccaggtgat cgtgctcaac ccggtgcgcg agctcggcct cgaacggttc cggatcccct
     4381 cgcagccctg gtcgttcgtc ttcgggtcga aggtgtccga cctctacctc cagccgaaga
     4441 tcggcggcga catcgccgtc ctgaagggga tgctcaaggc gctgctcgcg cgcgagggcg
     4501 ccatcgatcg atcgttcctg gcctcgcatg tcgacgggtg gcccgccgtc gagtcggacg
     4561 cgatggccac ctcgtgggaa acgatcgagc gccactccgg actcacgcgg gccgccgtcg
     4621 aggacgccgc cgcccgctac gcgaattcga agaaggccct cttcatgtgg gccatgggga
     4681 tcacccacca cgagcacggc gtggacaacg tcttcgccat caccaacctg gccctggccc
     4741 gcggcatggt gggaaaaccg cacgccggcg tcatgccgat ccgcggccac tccaacgtgc
     4801 agggaatcgg ctcggtgggg gtggcgcccg cgctcaagga agccttcgcc gagcggctca
     4861 cgcgcctgta cggcatcgag gtgccgaaaa cccccggcct cgacacgtat cgcaccatcc
     4921 tggccatgaa tgacggcacg atcaccggcg ccttctgcct cggcggcaat ctcttctcat
     4981 cgaaccccga tacggacttc acgcgcaccg cgatgcagaa ggtggagacg accgtgtacg
     5041 tgtccacgaa gttgaacccg ggccacttcc acggccgcgg gcgcacgacg tacatcctcc
     5101 cctgcctcgc gcgcgatgaa gagcaccagg ccacctcgca ggagagcatg ttcaactatg
     5161 tgcgcctctc cgaagggggt tccgaaccgg ctaccgccga catgaagagc gaagtcgaga
     5221 tcatcgccgg cctggccgcc cgctggctcc cgaagggaaa ggtggactgg tcccgcttca
     5281 cctcgcacga cgccctccgc gccgccatcg ccgatgtggt gcccggctgg gaagacttgc
     5341 gcaacatggg aacaacgaag cgcgaattca ccatctccgg ccgcgtgcgt cacgttcccg
     5401 aattcccgct ccccggcggc aaggcccggg cgttcgtgac gccgatgccc gacaaccttc
     5461 ccccacccgg tcacctcatg ctgatgacgc tccgtagcga ggggcagttc aacacggtgg
     5521 tgtacgacaa ggaagacatc taccgcgcca tcgaccggcg cgatgtggcg atgctgaacg
     5581 cggacgatgc gatgcggatg ggcttcgccc atgatgacat cgtaagagtc gaaacgaccg
     5641 cgggcgcgct ggacgggctc cgggtgcgaa tcctggacgt gccggccggc agtctcgtca
     5701 tgtactaccc ggaagccaac gccatcgtgc cgcggaagat cgaccccaag tcgggcacgc
     5761 cggcgttcaa gagcgtgccg gcgcgggtgg tgaagtcggt ggggcggccg gcgcctcatc
     5821 atgcgcggaa ggcttccgaa agcgtcgtgc cggtgtgaaa gggccacgcc cgccttcggc
     5881 tgaggggggc gtggttggtt gattcgcgca ctgtcttaca atgtaagaca accggttgag
     5941 catgtaaccg ccggccgcag ccacctccgt catcacgacc ggcgaataac aattcggccc
     6001 gacctccgtc aggaggccgg gccattctgt tttcgtcttg tgacgtcgtc gttccgcccc
     6061 gcggaaagtc cgttaccttg cgctcgtcac ccgcacgtcg cccgagcccg tctcgatgat
     6121 cacgttcccc ttgccgtcgc cgacggtgcc gcggaattcg gagcggcgat ttcgggtggc
     6181 ctggtggggt gcgtcgatgt cgatgtcgcc ggagccggtt tcgatgtcga ggctggcgcc
     6241 gaggtcgggg ccgacttcga gggtgacgtc gccggaaccg gtgtcgaagt tgccgccgtc
     6301 caggtcgccc tggatggcga cgaggatgga gccggaaccg gtgtcgcagg agatcttgct
     6361 gacggaaacc gactcgatgt tgatgtcgcc cgagccggta tcgaaggaga gctggtcgca
     6421 ggaaaggtga tctccctcga tgtcgcccga gccggtgtcg gcgtcgacca tgtcaccctt
     6481 cacgtcgctc aggatgacgt tgcccgagcc ggtgtcgacc gacacggtgc cgtcgattcc
     6541 ggtgagctcg acgtcgccgg aaccgcagtc gacgacgacg ctgccggtga cgccggtggc
     6601 ttcgacatcc cccgaggcgg tgtcgaggtt gagctcgccg gagacttcac tgaccaccac
     6661 cttgccgatg gcgttgtaga gcttcaggtc ggcgcccttg ggcatttcga tgcggaggtc
     6721 ggcccacatt tccttgccgg agccgctgcc cgagaccttg acctggtggc gtccctcgcg
     6781 cgaggagccg ccgaaggtgc cgtcgccgtt cattgaaatg gaggagttcg agcccatgcc
     6841 gagcgccggg tagac
//
LOCUS       DASVOD010000394         6654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_599517, whole genome shotgun sequence.
ACCESSION   DASVOD010000394 DASVOD010000000
VERSION     DASVOD010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599517"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VF720_08975"
     CDS             complement(<1..205)
                     /locus_tag="VF720_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879530.1"
                     /translation="MDEVADGAPLTQDAEVDARPPVRLALVAGVLAFLLYTLTSPAGI
                     SWEHSGEDGPEFAAVAKVLGVAHP"
     gene            complement(205..1491)
                     /locus_tag="VF720_08980"
     CDS             complement(205..1491)
                     /locus_tag="VF720_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879531.1"
                     /translation="MTTPEFHRLDEIDDALLLDAPMDDAAAGGRVEPWRLAAHERLRP
                     AALVRTDPDITRLVQSIALERERFQPWIGDAPATWDEVRGVLKHDADRSRREAAWFAG
                     VPLAMAVEADLQRLMQLRNERAAEVMDDSFPVAALRADELTVLEFMTWCDELEPLTRP
                     AFEQMLAWMHRENGGETLEPWDVAYWLARLEPGPESIRPIGLAKMEEELALPPTPPGA
                     AVPPPGAPDAEGRFEEWAAIGGHFHRRFRPKEPPAAGWTTGPWNEAMKRLVGETMATE
                     PILDRPGTLADRAWIAWRQVYALRRLMAASLFELLAYESPGADLHGLWCEIHEHYLGF
                     PRHPERMWAAEARFVTTPLAGAGLVVGRVMAAQLAERMLETHPHLLTPETGLFLREEL
                     WAPGATETAEAGVERVTGRIPDPEAWVRSVTVAPPS"
     gene            1562..2416
                     /locus_tag="VF720_08985"
     CDS             1562..2416
                     /locus_tag="VF720_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEX7879532.1"
                     /translation="MKPMILTHGGAGAPADLVDGPEAAARAAMALLLAEGDPLEAAIE
                     AARVLEDDPRFNAGTGSNLRLDGARIQMDASLMSDDGRFGAVLALERTKNPILVARAV
                     MDTPHLMIAGEGAINLARRLGFPDYDPTTPRARQKYERWRLESPNVPHDRWRNFPEDV
                     RAHDTIGAVVRGRDGRFAVSCSTGGTTMMLLGRVGDSPIPGAGLYAGPAGAVTATGEG
                     EEIIRRLLSLRVYERMARGESCAEAGAAELAAVPKEIAVGLIAVGTRDGWGGSNRQMP
                     FCVLESEE"
     gene            2446..3111
                     /gene="ubiE"
                     /locus_tag="VF720_08990"
     CDS             2446..3111
                     /gene="ubiE"
                     /locus_tag="VF720_08990"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEX7879533.1"
                     /translation="MFDSIARRYDLLNRVLSMGLDGGWRKRALSELALKPNMVLLDLC
                     TGTGDFGLEALAEHDLRIIGIDVAQEMMRVGLAKVIRQHVDHRFRFALGDAENLPLAD
                     KSIDGATIGFGIRNVVNRGRALGELSRVLKPGARLAILEFGVPPNPAFRDTYFLYATH
                     LLPVIGGALSGNRAAYEYLPDSIRKFPAPEEFGRLGTEAGFKTHKVRPLAFGIVNLYV
                     FSN"
     gene            3207..3575
                     /locus_tag="VF720_08995"
     CDS             3207..3575
                     /locus_tag="VF720_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018478385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX7879534.1"
                     /translation="MITITERARAHVLKLIQDNNLGDVHLRLGVTGGGCSGFNYNLTF
                     EKDVKPGDKAYEFADLTVVVDKLSRPFVGDTEIDFAESLYGSGFQFNNPKAESTCGCG
                     QSFSVAEDASGEPAAETVES"
     gene            3592..4299
                     /locus_tag="VF720_09000"
     CDS             3592..4299
                     /locus_tag="VF720_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7879535.1"
                     /translation="MKLLLFDIDGTLMTGGAGRRSLGMAFGEVFRLDAGPLDAAMKRV
                     PFNGRTDPAIIRDVAREAGLTDDEFEGGMERLYETYLRHLDTNSREPGPALCPGVPEL
                     LDQLESRAGIGLGLLTGNIEPGARIKLRGFDLNRYFPAGGFGSDSADRAIIASTARHR
                     FEVLLGAPIPAESVVVIGDTVHDVACGRVNGFRTLAVGTGGVALESMAEAGADMVLAD
                     LADTDGVLRHLSDLINI"
     gene            complement(4330..4686)
                     /locus_tag="VF720_09005"
     CDS             complement(4330..4686)
                     /locus_tag="VF720_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX7879536.1"
                     /translation="MEKKLREKIESTGANKIRRHIFLCCDQAKPKCCSYKEGMASWEF
                     LKHRLKELDLVGDGGIFRTKANCLQICTAGPIAVVYPEGTWYRDCTPKVLERIIQEHL
                     IGGQPVEEYVFLTQPL"
     gene            4808..5302
                     /locus_tag="VF720_09010"
     CDS             4808..5302
                     /locus_tag="VF720_09010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEX7879537.1"
                     /translation="MPSTPELFRAALGRFASGVTVVTSLDAAGRPNGLTVTAFSSVSL
                     APPLVMVGIDLANDSGPAIRHTGRFNVHLLKSGHEGWSRRFSERPNGADPFIGLDLGR
                     DAHGLPCLPDPLAFLACRVVHDIPAGDHVLFIGQVEEINVSDGDPLLYFRGGYRALDM
                     EPSQ"
     gene            5299..6393
                     /locus_tag="VF720_09015"
     CDS             5299..6393
                     /locus_tag="VF720_09015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX7879538.1"
                     /translation="MTLDIREHVPLAPFTTFGIGGPARRFLDAPSTDIVKAGLETARA
                     NGWPVFLLGGGSNLLVSDAGIDALVLRVSVTGKGPAGSVDPLLRFPEVPDHPQVRFIE
                     AGAGENWDDFVTWAVAHEMGGLECLAGIPGTVGGTPVQNVGAYGQEVSSIIRQVRALD
                     RTTGAIVRLDNADCGFHYRESRFNTTDLGRWIVLSVVFALPVGGAPNLSYRDVAEAFA
                     GRTTPPPLAEVRNAVRGIRQKKGMLLVDGDPDCKSAGSFFKNPRMTVAEADRLAGLVP
                     APLPLFPAGEGFRKVAAARLIEAAGFPKGHRRGNAAISSKHTLALTNQGGATAREVIE
                     LAREIRDGVRDRFGVTLVNEPVLVGFDEPL"
     gene            complement(6390..>6654)
                     /locus_tag="VF720_09020"
     CDS             complement(6390..>6654)
                     /locus_tag="VF720_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019883228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879539.1"
                     /translation="RVIFADNRFAEASSTPISRRDDFGNTWQRRRLEDGSEFEIVKNF
                     PTPEELSAVTRTHRTGVTIRLFDYYWILEGDTIPVPPEPAPEG"
BASE COUNT         1029 a         2407 c         2152 g         1066 t
ORIGIN      
        1 tgggatgcgc cacaccgagg accttggcca ccgccgcgaa ctccgggcca tcctctccgg
       61 agtgctccca cgagatcccg gccggcgacg tgagcgtgta gaggaggaac gccagcacgc
      121 cggccaccag cgcgaggcgg accggcgggc gggcgtcgac ctccgcgtcc tgcgtcagcg
      181 gggcgccatc cgctacttcg tccatcagga cggcggcgcc accgtgacgg accggaccca
      241 cgcctccgga tcgggaatcc gcccggtcac ccgctcgaca ccggcttccg cggtttcggt
      301 ggcgcccggc gcccacagtt cctcgcgaag gaagaggccc gtctccggcg tgaggagatg
      361 cgggtgcgtt tccagcatcc gctcggccag ctgcgccgcc atgacgcgcc ccaccacaag
      421 gcccgcgccc gccagcggtg tcgtaacaaa gcgcgcctcg gccgcccaca tccgctccgg
      481 atgccgcgga aagccgaggt aatgctcgtg gatttcacac cagaggccat gcaggtcggc
      541 gcccgggctt tcgtaggcca ggagttcgaa gagcgaggcc gccatcagcc gccgcaacgc
      601 gtacacctgc cgccaggcaa tccaggcccg atcggcgagt gttccggggc gatccaggat
      661 cggctcggtg gccatcgttt cgcccacgag ccgcttcatc gcctcgttcc aggggccggt
      721 cgtccaaccg gcggccggcg gctcctttgg ccggaagcgc cgatgaaagt gaccgccgat
      781 ggccgcccac tcctcgaagc gcccctccgc atccggcgcc cccggtggtg gaaccgcggc
      841 tccggggggc gtgggcggaa gggccagctc ctcctccatc ttcgccagcc cgatcggccg
      901 gatcgactcg gggcctggct cgaggcgcgc cagccaatac gccacatccc acggctcgag
      961 cgtttcaccg ccattctcgc gatgcatcca ggccagcatc tgctcgaagg cggggcgcgt
     1021 cagcggctcg agttcatcgc accaggtcat gaactcgaga accgtcaatt cgtccgcgcg
     1081 aagcgcggcc acgggaaagg aatcgtccat cacctccgca gcccgctcgt tccggagctg
     1141 catcaaccgc tgcaggtcgg cctcgacggc catggccagc ggcacgccgg cgaaccaggc
     1201 cgcctcccgg cgggaacggt cggcgtcgtg cttgaggacg ccgcgcacct cgtcccaggt
     1261 ggccggggcg tcgccgatcc agggttgaaa gcgttcgcgt tccaacgcga tcgactggac
     1321 cagccgcgtg atgtcgggat cggtccggac cagggccgcc gggcgaagac gttcatgcgc
     1381 ggcaaggcgc cacggctcga cacggccgcc ggccgccgca tcgtccatcg gcgcgtcgag
     1441 caggagcgcg tcgtcgatct cgtccagtcg gtggaactcc ggcgtcgtca tcggcggccg
     1501 attctactac caaggccggg tcatcgacgc atccggccgg gcgggctacg atgtcgcccc
     1561 gatgaaaccg atgatcctga ctcacggcgg cgccggcgcc cccgccgacc tggtggatgg
     1621 cccggaagcc gcggcccgtg ccgccatggc ccttctgcta gccgaaggcg atccgctcga
     1681 ggccgcgatc gaagccgccc gcgtcctcga agatgacccg cgcttcaacg ccggcaccgg
     1741 ctcgaacctc cgcctggacg gcgcccgcat ccagatggat gcctcgctca tgtccgacga
     1801 cggccgcttc ggcgccgtgc tcgcactcga gcggacgaaa aacccgatcc tcgtggcccg
     1861 cgcggtgatg gacacgccac acctgatgat cgccggcgaa ggggccatca acctggcccg
     1921 acgcctcggc tttcccgact acgaccccac cacgcccagg gcccggcaga aatacgagcg
     1981 ctggcggctc gagagtccga acgtaccgca cgaccggtgg cgcaattttc ccgaggacgt
     2041 gcgcgcgcac gacaccatcg gcgccgtcgt ccgcggcagg gatggccgct ttgccgtgag
     2101 ctgctcgacc ggcggtacca ccatgatgct gctcggccgc gtcggcgatt cgccgatccc
     2161 gggggccggc ctctacgcgg ggccggccgg cgccgtcacg gccaccggtg agggagagga
     2221 gatcatccgc cggctcctct cgctgcgcgt gtacgaacgg atggcccgcg gcgaatcgtg
     2281 cgccgaggcc ggcgccgccg agctcgcggc cgtgccgaag gaaattgccg tcgggctcat
     2341 cgccgtcgga acgcgcgacg gctggggtgg cagcaatcga cagatgccct tctgcgtgct
     2401 cgagagcgag gaatgacgcg cgatccccgc gatggaggcc cgatcatgtt cgactccatc
     2461 gcgcgccgct acgacctgct caatcgcgtc ctctccatgg gcctcgacgg cggctggcgg
     2521 aagcgcgccc tctccgaact ggcgttgaag cccaacatgg tgctgctcga tctctgcacc
     2581 ggcaccggcg acttcggcct cgaggcgctc gccgagcacg acctccggat catcggcatc
     2641 gacgtggcac aggagatgat gcgcgtgggc ctggccaagg tcatccgcca gcatgtggat
     2701 catcgctttc gctttgcgct cggcgatgcc gagaatctcc ccttggccga caaatcgatc
     2761 gacggcgcca ccatcggctt cggcattcgc aacgtggtca accgcggccg cgcgctcggt
     2821 gagctctcgc gtgtgctcaa gccgggcgcc cgcctggcca tcctcgaatt cggcgtcccg
     2881 ccgaacccgg ccttccgcga cacctacttc ctgtacgcca cccatctcct accggtcatc
     2941 ggcggggccc tctcggggaa tcgggcggcc tacgaatacc tccccgactc gatccggaaa
     3001 tttcccgccc cggaggagtt tggccgactc ggcaccgagg ccggcttcaa gactcataag
     3061 gtacggccgc tggcctttgg aatcgtaaac ctttatgtat tcagcaatta ggattgcctg
     3121 tgcccggggc ggcccgaaac gcccgaatcg aacccgcggc gggcgcctct caccccttgg
     3181 tatacttgtc ggctggaggt aacgacttga tcacgatcac cgaacgggcg cgcgcccacg
     3241 tcctcaagct cattcaggac aacaacctgg gcgacgtcca cctccgcctc ggcgtgaccg
     3301 gcggcggctg ctcgggcttc aactacaacc tcacgttcga aaaggacgtg aagcccggcg
     3361 acaaggccta cgaattcgcc gacctcaccg tggtcgtgga caagctctcg cgccccttcg
     3421 tgggcgatac cgagatcgac ttcgccgagt cgctctatgg ctcgggtttc cagttcaaca
     3481 acccgaaggc cgagtcgacc tgcggttgcg gccagtcatt ctcggtggcg gaagacgcct
     3541 ccggcgaacc ggccgccgag acggtcgaat cctgacccct ctcccccgct cgtgaagctt
     3601 ctcctcttcg acatcgacgg cacgctcatg accgggggcg ccggccgccg gagcctcgga
     3661 atggctttcg gcgaggtgtt ccgcctcgat gccggccccc tcgacgccgc gatgaagcgg
     3721 gtgcccttca atggacgcac cgacccggcc atcattcgtg atgtggcccg cgaagcgggc
     3781 ctcaccgatg acgagttcga aggagggatg gagcgcctct acgagaccta cctccgccat
     3841 ctcgatacca acagccgtga accgggcccc gccctctgcc ccggcgtgcc ggaactgctc
     3901 gaccagctcg aatcacgcgc cggcatcggc ctggggctcc tgaccggcaa catcgagccg
     3961 ggggcgcgca tcaagttgcg gggcttcgat ctcaatcgct acttccccgc cggtggtttc
     4021 ggcagcgact cggccgatcg ggccatcatt gcctccaccg cccggcatcg cttcgaggtg
     4081 ctcctgggcg cgccgatccc ggcggaatcg gtggtggtga tcggcgacac cgttcacgac
     4141 gtggcctgcg gacgcgtgaa tggcttccgg acgctggcgg tggggaccgg gggtgtggcg
     4201 ctcgagtcga tggccgaggc cggagccgac atggtcctgg ccgacctggc ggacacggac
     4261 ggggtgctca ggcacctgtc cgatcttata aatatttaat tcacaacaat ttacatcgtt
     4321 caggcgggac tacaggggct gcgtcaggaa gacatattcc tcgaccggct gccccccgat
     4381 caggtgctcc tggatgatcc gctcgagcac cttgggggtg cagtcccggt accaggttcc
     4441 ttcgggatag accaccgcga tcggccccgc ggtgcagatc tgcaggcagt tggccttggt
     4501 ccggaagatg ccgccatccc ccaccaggtc gagctccttc aagcggtgct tcaggaattc
     4561 ccaggaggcc atcccctcct tgtagctgca gcacttgggc ttggcctgat cgcaacagag
     4621 gaaaatgtga cggcggatct tgttcgcgcc ggtcgattcg atcttttcgc ggagcttctt
     4681 ttccatccct cgattgtagc ccacacgccg gccgcttcgg ccacccgcac tcgccccttg
     4741 cgccacccgc atgccccccg agccggttga atcgcaccct ctccgcccct atcgttccgg
     4801 caccgccatg ccatccacac ccgagctctt ccgcgcggcc ctcggccgct ttgcctccgg
     4861 cgtcaccgtc gtgacctcgc tcgacgcggc cgggcgcccg aacggcctca cggtgacggc
     4921 tttttcctcg gtgtcattgg cgccgccgct cgtgatggtc ggcatcgatc tggccaacga
     4981 ctcgggcccg gccatccggc acaccggccg cttcaacgtc cacctcctca agtccgggca
     5041 cgaggggtgg tcgcgccgct tcagcgagcg tcccaacggg gcggacccgt tcatcggcct
     5101 cgacctcggc cgcgacgcgc acggactccc ctgccttccc gacccgctcg cgttcctcgc
     5161 ctgtcgcgtc gttcacgata tcccggccgg cgaccatgtg ctcttcatcg ggcaggtgga
     5221 ggagatcaac gtgtcggacg gcgatccact cctctacttc cggggcggat accgcgccct
     5281 cgacatggaa ccttcgcaat gacgctcgat atccgcgagc acgtgccgct ggcgccgttc
     5341 accacgttcg gcatcggcgg cccggcccgg cgtttcctcg acgcgccatc caccgacatc
     5401 gtgaaggcgg gcctcgagac cgcccgcgcc aacggctggc cggtctttct cctggggggc
     5461 ggcagcaatc tcctggtgtc ggacgcggga atcgacgcgc tcgtcctgcg cgtctccgtc
     5521 acggggaagg gaccggccgg cagcgtcgat ccgctgctgc gctttcccga ggtgcccgat
     5581 catccgcagg tgcgcttcat cgaggcgggc gccggcgaaa actgggacga cttcgtcacc
     5641 tgggcggtgg cccacgaaat gggggggctc gagtgcctgg ccggcatccc cggcacggtg
     5701 gggggcacgc cggtgcagaa cgtgggcgcc tacggccagg aggtgtcgtc gatcatccgc
     5761 caggtgcgcg cgctcgaccg caccaccggc gcaatcgtgc gcctcgacaa cgccgactgc
     5821 ggattccact atcgcgagag ccgcttcaac accaccgacc ttgggcgatg gatcgtcctc
     5881 tcggtcgtgt tcgcgctgcc ggtggggggg gcgccgaact tgagctaccg cgacgtggcc
     5941 gaggccttcg cgggccgcac cacgccgccg ccgctggccg aagtgcggaa cgcggtccgc
     6001 ggcatccggc agaagaaggg gatgctcctc gtcgacggcg atcccgattg caaaagcgcc
     6061 ggctcgttct tcaagaaccc ccgcatgacg gtggccgagg ccgatcgact ggccggcctc
     6121 gtccccgcgc cgctcccgct tttcccggcg ggggaggggt tccggaaagt ggcggccgcc
     6181 cggctcatcg aggcggcggg attcccgaaa gggcatcgcc gcggcaatgc cgccatctcg
     6241 agcaagcaca cgctggcgct caccaaccag gggggcgcca ccgcccggga ggtcatcgag
     6301 ctggcccgcg agattcgcga cggcgtgcgc gaccgattcg gcgtgacgct ggtgaacgag
     6361 ccggtcctgg tggggttcga cgagccgctc taaccctcgg gagccggctc cggcggcacg
     6421 gggatcgtgt ctccctcgag gatccagtag tagtcgaaca gccggatcgt gacgccggtc
     6481 cggtgtgtcc gcgtcactgc cgacaattcc tccggcgtcg ggaaattctt cacgatctcg
     6541 aactccgagc cgtcctctag gcgccggcgc tgccaggtat tgccgaagtc atcgcgacgc
     6601 gagatcggcg ttgagctcgc ctccgcgaag cggttgtcgg cgaagatcac ccgg
//
LOCUS       DASVOD010000395         6898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_809431, whole genome shotgun sequence.
ACCESSION   DASVOD010000395 DASVOD010000000
VERSION     DASVOD010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6898
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809431"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VF720_09025"
     CDS             complement(<1..675)
                     /locus_tag="VF720_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7879540.1"
                     /translation="MTTDTATGSLADLEALAAARQKIVTELRKAIVGQDQVIDELLIA
                     LFANGHCVLVGVPGLAKTLLISSLARSLELRFNRIQFTPDLMPSDITGTEIIEEDKTS
                     GRRVFKFIQGPIFANVVLADEINRTPPKTQAALLQAMQEREVTTGGQTYPLKPPFFVL
                     ATQNPIELEGTYPLPEAQLDRFMFMVHVDYPNQEDEVKIVSSTTSAYESNVQSIMSGD
                     DIIRYQK"
     gene            complement(830..1693)
                     /locus_tag="VF720_09030"
     CDS             complement(830..1693)
                     /locus_tag="VF720_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879541.1"
                     /translation="MTRRPPAKKRPRAALTAKTADLHLLYERSVQSIDVDLDFADRVY
                     REHRKAPLRVLREDFCGTAALASNWVRRHPKNRASGVDLHGPTLEWGLKEHVSRMTPD
                     QQSRLLLLQENVLTAATPPADLVLALNFSYSVFHDRAALRAYFKAAHAALNEKGLLVV
                     DVFGGTESMIAWSEKRHLPAALTVEGDPIPAFTYMWEQAAFNVVDHRTVCHIHFKFKD
                     GSVRKKAYTYDWRLWTLPELAEIMVEAGFRKTVTYLHGWTKDGDSDGHFRKRTRFENA
                     ETWLAYVVGVR"
     gene            complement(1817..2359)
                     /locus_tag="VF720_09035"
     CDS             complement(1817..2359)
                     /locus_tag="VF720_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX7879542.1"
                     /translation="MSKAVLCLTNTTGQAAAIVDDLRAVGFSNNDISVLYPDKSGTRD
                     FAHEHHTKAPEGAASGAGAGGVVGGTLGLLAGIGTLAIPGVGPFVAAGPLMAALSGAA
                     VGAAVGGITGTLIGMGIPEYEAKQYEGKIRDGKILISVHTEDGQERDRAKDVFKAAHA
                     EDIGIADEKGKDTIKEKDYQ"
     gene            complement(2428..2850)
                     /locus_tag="VF720_09040"
     CDS             complement(2428..2850)
                     /locus_tag="VF720_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX7879543.1"
                     /translation="MSRSLMAAMLALSTMAVACSNSNHDAAQNKAPDYSQNQPANTQA
                     DNTAQNKVDQTNTVPEPMDQGTSDVDMTITTVLRKAITSDESLSMNAKNVKIITRDGV
                     VTLRGPVETTAERAAIEAAANAVSGVRRVDSYLEVTGG"
     gene            3325..3714
                     /locus_tag="VF720_09045"
     CDS             3325..3714
                     /locus_tag="VF720_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX7879544.1"
                     /translation="MPDHRSASWFTRFARNTARLSGRPGTFGLAVAIILVWGVTGPLF
                     HFSDTWQLVINTGTTIITFLMVFLIQNTQNRDSEAVQIKLDELVRAVQGARNALIDLE
                     ELDDKELERVQEMYRNLARTAREDDPI"
     gene            3864..4832
                     /locus_tag="VF720_09050"
     CDS             3864..4832
                     /locus_tag="VF720_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7879545.1"
                     /translation="MSSAVLVRNVRKAYDKHVAVEDVSLEVPQGIVFGLLGPNGAGKT
                     TTIRMIMDIIRPDSGSVEIFGGTPNTVTLDGIGYLPEERGLYKRMKVLDVLGYLGSLK
                     GIEAAEARRRAVTWLDKVGLAAWKERKIEELSKGMAQKVQFVSTLLHEPKLLILDEPF
                     SGLDPVNANQLKDLFVEVNRAGATIIFSTHMMESAEKLCRQIALISKGRVVLEGSIGD
                     VKRHYGRNNVLLEFDGDSGFLKALPGVKGVDDYGAYSEVMLKDGADPQELLAGVAPRV
                     KLRRFEIVEPTLHNIFIQVAGPDAAEALKASEAASHAAVSGAGGAR"
     gene            4829..6115
                     /locus_tag="VF720_09055"
     CDS             4829..6115
                     /locus_tag="VF720_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7879546.1"
                     /translation="MSFARIWLVARREYKEHVAKKSFWISLIVPPILFTFVILLQIAS
                     FRARPEETRNLVLVDNSGKLGSVIAADLGDDTFKSGKNQFQVEMRTGEADTTALMADL
                     NRRISQKEIFGYLLIGPNLEAPGAYRLFLRNVGDAVTTDKVEDALRRAVIGARLTDRQ
                     LSITGADLDSITKRVPLMTLKVDEKGKVSRGAFMLVWISTFVFLMFMFMPIIAYGVTA
                     LRSVLEEKSSRIIEVLLSSLTPFQLFSGKILGLALVGLTQVGAYVVTSLLFSAYSRSM
                     APAGMLKDIGGVITPVVLGLFLIYFLLGFLLFLTIFTAIGSMVNNEQEATHMQQPVIM
                     MLVLPMYAVFFFISNPDSTLARVMSMIPVFSPMIMMMRIMALMPPWWEIAASVLIISA
                     AIVGTAWAASRIFRVGVLMYGKRPTLPEIIKWVKTG"
     gene            6097..6852
                     /locus_tag="VF720_09060"
     CDS             6097..6852
                     /locus_tag="VF720_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX7879547.1"
                     /translation="MGEDRVDTAVVLVEPRNAGNVGLVARTMANFGVARLVLAGSTEL
                     PAEKDQLAARAEGRPILDALVRAADLAAALADEQVTIAVTRRTGKERPGDLFPATLAE
                     FRAGLPPGTRVAFVFGRESDGLRTDEARLCARRLRIATTEEAPSLNLAQAVAVVLGRW
                     YESVAAAGPGTLPTRRGIPPSPVNDPLASNARFEEMMAALEPALRAIGYLEGDRDEDT
                     MNSLRRLFGRAQLTEREIRMLRGLATKIGKGRE"
BASE COUNT         1127 a         2254 c         2298 g         1219 t
ORIGIN      
        1 cttctggtag cggatgatgt cgtcgcccga catgatcgac tgcacgttcg attcgtaggc
       61 gctggtggtc gaggagacga tcttcacttc atcttcctgg ttcggataat ccacgtgcac
      121 catgaacatg aagcggtcga gctgggcttc cggcagagga taggtgccct cgagctcgat
      181 cgggttctgc gtggccagca cgaagaacgg cggcttgagt ggatacgtct ggccgccggt
      241 cgtcacctcg cgctcctgca tggcctggag cagtgcggcc tgcgtcttgg ggggcgtgcg
      301 attgatctcg tcggccagca ccacgttggc gaagatcggg ccctggatga acttgaagac
      361 gcggcggccg ctcgtcttgt cttcctcgat gatttcggtg ccggtgatgt cgctcggcat
      421 gaggtcgggc gtgaactgga tgcggttgaa gcggagctcg aggctccggg ccagcgacga
      481 gatgagcagc gtcttggcca gtcccggcac gccaaccagc acgcagtggc cgttggcgaa
      541 gagggcgatc aggagctcgt cgatcacctg gtcctgcccg acgatggcct tccggagttc
      601 ggtgacgatc ttctggcggg cggcggccag ggcttcgagg tcggccaggc tcccggtggc
      661 ggtatcggtg gtcatgtgtc gattccttgc ggcggtgccg cccagttgaa ccgcccggtg
      721 gcggcaggtc gataacgggc cagagccaag caggataaga gattccgggg cccctggcga
      781 gcccgggggg cgggatgaac cccgggccgg gagcccggtt cgtgcgtaat taccggacgc
      841 ccaccacgta ggccagccag gtctcggcgt tctcgaaacg ggtccgcttc cggaagtggc
      901 cgtccgagtc gccgtccttc gtccagccgt ggaggtaggt caccgtcttc cgaaaaccgg
      961 cctccaccat gatctcggca agctcgggga gggtccagag gcgccagtca taggtatagg
     1021 ccttcttccg aacgctccca tccttgaact tgaagtggat gtggcagacg gtccggtggt
     1081 ccaccacgtt gaaggcggcc tgctcccaca tgtaggtgaa ggccgggatc ggatccccct
     1141 ccaccgtgag ggccgccggc aggtgccgct tctccgacca cgcgatcatc gattcggtgc
     1201 cgccgaagac atccacgacc aggagcccct tctcgttcag ggccgcgtgc gccgccttga
     1261 agtaggcgcg cagggccgcg cggtcgtgaa agaccgagta cgagaagttg agcgccagga
     1321 cgaggtcggc cggcggtgtg gcggcggtca acacgttctc ctgcagcagc aggaggcgcg
     1381 attgctggtc aggcgtcatc cgcgacacgt gctccttgag tccccactcg agcgtggggc
     1441 cgtgcaggtc cacgccgctg gcccggttct tcggatgacg ccgcacccag ttcgaggcca
     1501 gcgccgccgt gccgcagaaa tcctcgcgca gtacgcggag cggcgccttc cggtgctcgc
     1561 ggtacacccg gtcggcaaag tcgagatcga catcgatcga ctggacggag cgttcgtaca
     1621 ggaggtggag gtcggcggtc ttggcggtca gcgcggcgcg cgggcgcttc tttgcgggag
     1681 gtcggcgtgt catcgggtcg gggaaggtag cagcccggag gttgcccgga aaagggcagg
     1741 accccggttg ccgggacggc ggcaaccggg gtccaaccaa ctgcgggggt gaagtcgtca
     1801 gctggcgccc tgaactctac tgatagtcct tctccttgat cgtgtccttc cccttctcgt
     1861 cggcgatgcc gatgtcctcg gcatgggccg ccttgaagac gtccttggcg cggtcgcgct
     1921 cctggccgtc ctcggtgtgg accgagatga ggatcttgcc gtcacggatc ttcccctcgt
     1981 actgcttggc ttcgtactcg gggattccca tgccgatcag ggtgcccgtg atgccgccga
     2041 ccgccgcacc gaccgcggca ccgctcagcg ccgccatcag cgggccggcc gccacgaacg
     2101 gtccgacgcc gggaatggcc agcgtgccga tgccggccag gaggccgagg gtgccgccga
     2161 ccacaccacc ggcgcccgcg ccgctggccg cgccttccgg cgccttggtg tggtgctcat
     2221 gggcgaagtc acgggtgccg ctcttgtccg gatagagcac cgagatgtcg ttgttcgaga
     2281 agccgacggc gcgcaggtcg tcgacgattg ccgcggcctg gcccgttgtg ttcgtgaggc
     2341 agagaacagc cttcgacatg atctcctcct tggttgggag agttgtgtgg tttcgtccgg
     2401 aaggtcctgg ggcggctggt tgccgaccta gccgccggtc acctcgagat acgaatcgac
     2461 gcgacggaca ccgctcaccg cgttggccgc ggcttcgatg gccgcgcgct cggccgtggt
     2521 ttcgaccggg ccgcggaggg tcaccacgcc gtcacgcgtg atgatcttca cgttcttcgc
     2581 attcatggac agggattcgt ccgaggtgat tgccttgcgg aggaccgtcg tgatggtcat
     2641 gtccacatcg ctggttccct ggtccatcgg ctccggaacg gtgttcgtct ggtccacctt
     2701 gttctgggct gtgttgtcgg cctgcgtatt ggccggctgg ttctggctgt aatcgggtgc
     2761 cttgttctgc gcggcgtcgt ggttcgaatt cgagcaggcg accgccatcg tcgaaagcgc
     2821 cagcatcgcc gccatcaggg atcgagacat cgcccgtcct ccttctacat cgtggtcaca
     2881 cttccggcgg cgctgagccg ccatcgtcaa ccggggagtc ggcgcacatt cgtgcgtcac
     2941 cccgcggacg gaggaaccgg aattgcaact cgtgtgcctc atgcgccgga cacggcgggt
     3001 gccggcattc cgtcggggaa cacgcgcccc acaaccgccc gcgaggcagc cgtttaggcg
     3061 atcgcaagat cggtttcgat gcctgcggac ggtggcggaa cccgcgcccg atgggagaaa
     3121 gcccgatgag aaatcgttac gtgcccgcga acaaccgcac gccggcgggc gtaaatttgt
     3181 ctggagggtc ggcggtcccg tggcgtggcc gtcagcgcgg aaaaacttca acgcgaggag
     3241 gagaggatgc ccggggaaca ggacacgatt cccgatgaac aaccgccgcc gccggacaag
     3301 caaaagcgcc ttgtccacga tgctgtgccg gatcaccgct ccgcttcgtg gttcacccgc
     3361 ttcgccagga atacggcccg actctccggc cggccgggca cgttcggcct ggcagtggcc
     3421 atcatcctgg tgtggggcgt taccggcccc cttttccact tcagtgatac ctggcagctc
     3481 gtcatcaaca cgggcacgac catcatcacg ttcctgatgg tcttcctcat ccagaacacg
     3541 cagaaccgcg attcggaagc ggtgcagatc aagctcgacg agctggtccg ggccgtgcag
     3601 ggcgcgcgga acgccctgat cgacctggaa gagctggacg acaaggagct ggaacgggtc
     3661 caggagatgt atcgcaacct cgcccggaca gcccgggagg acgacccgat ctaggcgcca
     3721 ggcgcggcga tcggcgcgca accgccccgc ccctgccgcg taaggcgccc ggtcgctata
     3781 ctgcccggcg cccggctgcg tccgcccccg atcgggacgg cacccgggtt cgtcacccgc
     3841 acgtccacct gatgggggtc cccgtgtcca gcgccgtcct ggtcaggaac gtccgaaagg
     3901 cctacgacaa gcacgtggcc gtcgaggacg tcagtctcga agtccctcaa gggatcgtct
     3961 tcggcctgct cggccccaac ggggccggca agaccaccac catccggatg atcatggaca
     4021 tcatccgccc cgactcggga tcggtggaaa tcttcggcgg cacgccgaac accgtcaccc
     4081 tggatggcat cggctatctc cccgaggagc gcggcctcta caagcggatg aaggtgctcg
     4141 acgtgctcgg atacctgggc tcgctcaagg ggatcgaggc cgccgaggcc cgccggcgcg
     4201 ccgtcacctg gctcgacaag gtggggctcg ccgcctggaa ggagcggaag atcgaggaac
     4261 tttcgaaggg gatggcgcag aaagtgcagt tcgtgagcac cctcctgcac gaaccgaagc
     4321 tcctcattct cgacgagccg ttcagcggcc tcgacccggt caacgccaac cagctcaagg
     4381 acctcttcgt ggaggtcaat cgcgccgggg ccacgatcat cttctccacg cacatgatgg
     4441 agagcgccga aaagctctgc cgccagatcg cgctcatcag caaggggcgt gtggtgctcg
     4501 aaggttcgat cggcgacgtg aagcgccatt acggacggaa caacgtcctc ctcgagttcg
     4561 acggcgacag cggattcctt aaggccctcc ccggggtgaa gggagtggac gactacggcg
     4621 cctacagcga ggtgatgctg aaggacggcg ccgatcccca ggaattgctg gccggggtgg
     4681 cccccagggt caagctccgc cgcttcgaga tcgtggagcc caccctgcac aacatcttca
     4741 tccaggtggc gggacccgac gcggccgagg cgctcaaggc ctcggaggcc gcttcgcatg
     4801 ccgccgtgag cggtgcggga ggagcccgat gagcttcgcc aggatctggc tggtggcgcg
     4861 tcgcgagtac aaggagcacg tcgccaagaa gtcgttctgg atcagcctca tcgtgccgcc
     4921 gatcctgttc accttcgtca tcctgctcca gatcgcctcg ttccgggcgc gccccgagga
     4981 aacccgcaac ctcgtgctcg tggacaattc cgggaagctc ggctccgtca tcgccgccga
     5041 cctgggggac gacacattca agagcggcaa gaaccagttc caggtggaga tgcgcaccgg
     5101 agaggccgac acgacggccc tcatggccga cctcaatcgt cggatcagtc agaaggagat
     5161 cttcggctac ctgctcatcg gcccgaatct ggaagcgccg ggcgcatacc gattgttcct
     5221 gcgcaacgtc ggcgatgccg tcacgaccga caaggtggag gacgctctcc gccgcgccgt
     5281 catcggcgcg cgcctcaccg atcgccagct ctccatcacc ggcgccgatc tcgattccat
     5341 cacgaagcgc gtacccctca tgacactcaa ggtggatgag aaggggaagg tcagtcgcgg
     5401 ggccttcatg ctcgtctgga tttccacctt cgtcttcctg atgttcatgt tcatgccgat
     5461 catcgcctac ggcgtcacgg cgctccggag cgtgctggag gagaagtcgt cgcgcatcat
     5521 cgaggtgctc ctctcctcgc tgacgccgtt ccagctcttt tcgggcaaga tcctcggcct
     5581 ggccctggtg ggactgacgc aggtgggtgc ctacgtcgtg accagcctcc tcttctccgc
     5641 ctacagccgt tcgatggctc cggccggcat gctcaaggat attggcggcg tcataacgcc
     5701 tgtcgtcctc ggcctgttcc tcatctactt cctgctcgga ttcctcctgt tcctcacgat
     5761 cttcacggcg atcggttcga tggtgaacaa tgaacaggaa gccacgcaca tgcagcagcc
     5821 ggtgatcatg atgctggtcc tgccgatgta cgccgtcttc ttcttcattt cgaaccctga
     5881 ttccacgctg gcacgcgtca tgtcgatgat tccggtgttc tccccgatga tcatgatgat
     5941 gcggatcatg gcgctcatgc cgccgtggtg ggagatcgcc gcatcggtcc tgatcatcag
     6001 cgccgcgatc gtgggcacgg cctgggccgc ctcccgcatc ttccgcgtcg gcgtcctcat
     6061 gtacggcaag cgccccaccc tccccgaaat catcaagtgg gtgaagaccg ggtagacacc
     6121 gccgtcgtcc tggtcgagcc gcgcaacgcc ggcaacgtcg ggctggtggc gcgcacgatg
     6181 gccaacttcg gcgtggcgcg gcttgtcctg gcggggagca ccgaactccc cgccgagaag
     6241 gaccagctcg cggcgcgggc cgaggggcgg cccatcctgg acgcgctggt gcgggcggcc
     6301 gacctggccg ccgcgcttgc cgacgaacag gtcacgatcg cggtgacgcg ccgcaccggc
     6361 aaggagcgcc ccggcgatct ctttcccgcc acgctcgcgg aattccgggc gggattgccg
     6421 ccgggcacgc gggtggcctt cgtcttcggg cgcgaatccg atggcctccg caccgacgag
     6481 gcacgcctct gcgcgcgccg gttgcgaatc gccacgacgg aggaggcgcc atccttgaac
     6541 ctggcacagg cggtggccgt ggtgctggga cgctggtacg agtcggtggc cgccgccggg
     6601 cccggaacgc tccccacgcg gcggggaatt cccccatcac cggtgaacga cccgctcgcc
     6661 tcgaacgcgc gcttcgagga gatgatggcc gcactcgaac ccgcgctccg tgcgatcggc
     6721 tatcttgaag gggatcgcga cgaggacacc atgaactcgc tgcgccgcct cttcgggcgg
     6781 gcgcagctca ccgagcggga aatccggatg ctgcgcggac tggcaacgaa gatcgggaag
     6841 gggcgggagt gaggcggggc gtcggcgccc gtcgcgtcgt gatgtctctc atgacgat
//
LOCUS       DASVOD010000396         3396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_235626, whole genome shotgun sequence.
ACCESSION   DASVOD010000396 DASVOD010000000
VERSION     DASVOD010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235626"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VF720_09065"
     CDS             complement(<1..1071)
                     /locus_tag="VF720_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879548.1"
                     /translation="MSGGGHPIAALLLALALSFTGCASRDHTNPFDPENDETDGRPDL
                     VVAAAGDGVVELSWVLPDFHDIDTVQLVRIDDANQETVLDVEVLGPDSLATDDDVVND
                     RTYRYLLEFRFDGDANPQRSAEAPATPGRTVIWVLDQTRGGPLRISSDGRVVAARPGR
                     GRPTDMSIESSTGRVASVDFINQRIETFDRTGRPLADALTIFNPQSVAIDEPGDGIWI
                     GAANPSTIQLRSADLSTIASADTGFGDPEDIAVDPLTGALWIADSGSGRLFHRRRDGT
                     VATIGGLSAPFSLSVDAATGDAFLADRARRTLFRVSALGDTVLWARPGFAGLYQALAD
                     PSTGGVWVSDNLAGSVTHVSGEG"
     gene            complement(1127..2188)
                     /locus_tag="VF720_09070"
     CDS             complement(1127..2188)
                     /locus_tag="VF720_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX7879549.1"
                     /translation="MKVATPRTFATSRPVAAFVLVVMSWSTVAPSMPWAAGALTAGDS
                     TGTADSTPVLAEPPSWYRLLVHQPGVILRDGEPMLDLTGTGGRRPIPWTSFTIAVVEV
                     PAGTHRLTLKPADPMAVPPYEGFVVTVEPGDTLTVRLGAPRVTTSPPGARVLLDGEDL
                     GPAPVWIDPTRLPGHKVLVEMAGYQRRTVEGDTLLDLARSSGAARFELEPLTPQPLVE
                     LQPPEASSWIGRNRGLALTGSFLLLGAGVAGGIYFKNKADDFYDDYEHTGNASERDRW
                     FNKAQQNDRASLLGWLVGEAAFFATFFLLIYQPPRGLVTNPDATPAPVEPVARSRAAL
                     APIAPDGEPGVTIGFVHEF"
     gene            complement(2185..>3396)
                     /locus_tag="VF720_09075"
     CDS             complement(2185..>3396)
                     /locus_tag="VF720_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7879550.1"
                     /translation="YRRDFLHRDELLMRIQVRAMRAWMTLILGRFLLLRDRLGAMSVS
                     VLPLAPLLLLAMTPGCGTYTRGEAIKPPTPITGLPPRVLWTADAGRGVDVTPTLYGNE
                     LFVATTDRLFTRYRPDSGERRWKKRLDGAVSGRPFVVGTVAYMATSLPDGAVYGIDLA
                     REEVVWRVTVGEPVGDPVFFEGAVIVGTQEGAVFGLSPSDGKTIWVTNIEDRVWGSTW
                     FDTSRAIFVMPGRGGRLSAIDGRSGERRWTIELGQPLGAASGDSARLFVVAGDSSLIA
                     LDPANGNRFWTQTLDFPLRAGVVPVGDRVIVAGLDGHVCALAVATGEPAWTLRLGGPF
                     VAPPAVSEGMLLIGSPSGLVWRIEVSSGAVTGLFRHDEPVLVTPAVDDSLWIVAGERG
                     RLVACRWEDGR"
BASE COUNT          556 a         1210 c         1158 g          472 t
ORIGIN      
        1 cccctctccg ctgacatggg tcaccgaacc cgccaggttg tcggacaccc acaccccgcc
       61 ggtggacggg tcggccaggg cctggtacag ccccgcgaac ccggggcggg cccagaggac
      121 cgtatccccg agggccgaaa cccggaagag agtgcgtcgc gcgcggtcgg ccaggaaggc
      181 atcccccgtg gccgcatcga ccgacaggga aaatggcgcg gagagtcccc cgattgtggc
      241 caccgtgccg tcgcgccggc gatggaaaag acgcccgctc cccgagtccg cgatccagag
      301 cgcgccggtc agcggatcga ccgcgatgtc ttcggggtcc ccgaatcccg tgtcggccga
      361 cgcgatggta ctgaggtccg ccgaccggag ctggatggtg gaagggttgg ccgcgccgat
      421 ccagatgccg tcaccgggct catcgatggc cacgctctgc ggattgaaga tcgtgagcgc
      481 gtcggccagc ggccgcccgg ttcggtcgaa cgtctcgatc cgttggttga tgaagtcgac
      541 gctcgcgacg cgcccggtgc tcgattcgat cgacatgtcg gtggggcgcc cccgccccgg
      601 ccgggctgcc accacgcgtc cgtcggacga aatgcggagc ggccccccgc gtgtctggtc
      661 gagcacccag atcaccgtgc gccccggcgt ggccggcgcc tccgcgctcc gctgcgggtt
      721 ggcatcgccg tcaaaccgga attccaggag atatcgatac gttcgatcgt tcacgacgtc
      781 gtcgtcggtg gccagcgagt cggggcccag cacctcgaca tccaggaccg tttcctggtt
      841 cgcgtcgtcg atgcggacga gttgcaccgt gtcgatgtcg tggaagtcgg gaagcaccca
      901 gctcaactcc acgacgccgt cccccgccgc ggccaccacc aggtcggggc ggccgtcggt
      961 ttcgtcgttt tccgggtcga agggattggt gtgatcccgg ctggcgcatc cggtgaatga
     1021 gagcgcgagg gcgagcagga gtgccgcgat gggatgccct cccccggaca cggggctgcc
     1081 cccgagggct ggggtgcccc aagtcgttcg gggacctgta ttcctttcag aactcatgca
     1141 cgaatccgat cgtcacgccc ggctcgccgt ccggcgcaat cggtgccagc gcggcacggc
     1201 tccgcgccac gggctcgacc ggcgcgggcg tggcgtccgg gttggtaacg aggccgcgcg
     1261 gcggctggta gatcaggagg aagaacgtgg cgaagaaggc cgcttcgccg accagccagc
     1321 cgagcagcga ggcgcgatcg ttctgctgcg ccttgttgaa ccagcgatcc cgctcggagg
     1381 cgttgccggt gtgctcgtag tcgtcgtaga agtcgtcggc cttgttcttg aaatagattc
     1441 cccccgcaac gcccgcgccg aggagaagga acgaaccggt aagcgccagg ccacgattcc
     1501 gcccgatcca gctcgaggcc tcgggcggct ggagttccac cagcggctgc ggcgtcagcg
     1561 gctcgagctc gaagcgcgcc gcgccggagg aacgtgccag gtcgagcagc gtgtctcctt
     1621 ccaccgtgcg ccgctggtat ccggccattt ccaccagcac cttgtgtccg ggaaggcggg
     1681 tcgggtcgat ccagaccggc gcggggccca ggtcctcgcc atccagcaac acgcgggcgc
     1741 ccgggggcga ggtcgtcacg cgcggggcgc ccaaccggac ggtcagcgtg tcccccggct
     1801 cgaccgtcac cacgaacccc tcgtacggcg gcaccgccat cggatccgcc ggcttgagcg
     1861 tcaggcgatg cgtccccgcc ggcacctcca ccaccgcgat cgtgaacgat gtccagggga
     1921 tcggacgccg gccccccgtg ccggtcaggt cgagcatcgg ctcgccgtcg cgcaggatga
     1981 cgcccggctg atgaacgagg aggcggtacc aggagggcgg ctccgcaagg acgggcgtgg
     2041 agtccgcggt gcctgtcgaa tcgcctgccg tcaacgcccc ggcggcccat ggcatcgaag
     2101 gagccacggt cgaccagctc atcaccacca gcacgaacgc ggcgaccgga cgcgaagtgg
     2161 caaacgtgcg cggggtggcc accttcaccg cccatcctcc cagcggcacg ccaccaggcg
     2221 tccgcgctcc ccggccacga tccagagact gtcgtcgacg gccggcgtga cgaggacggg
     2281 ttcatcgtga cggaatagtc cggtgaccgc gcccgaggag acctcgatcc gccacacgag
     2341 ccccgacggg gagccgatca gcagcatccc ttccgacacg gcgggaggcg ccacgaaggg
     2401 accgccgagc ctcagcgtcc aggcgggttc gccggtggcg acggccaggg cgcacacgtg
     2461 gccatcgagg ccggccacga tgacgcgatc gcccaccggc acgacgccgg cccggagcgg
     2521 gaagtcgaga gtctgcgtcc agaagcggtt gccgttggcc ggatcgaggg cgatgaggga
     2581 gctgtccccc gccaccacga acaggcgggc cgagtcgccg ctggcggcgc cgagcggctg
     2641 gccgagctcg atggtccaac gccgctcgcc gctccgaccg tcgatggccg aaaggcggcc
     2701 ccctcgtccc ggcatcacga agattgcccg gctcgtgtcg aaccaggtcg agccccagac
     2761 cctgtcttcg atattggtca cccagatcgt cttgccgtcc gatggcgaga ggccgaaaac
     2821 ggccccttcc tgcgtgccga cgatgaccgc cccctcgaag aagaccgggt cacccaccgg
     2881 ctcccccacc gtcacgcgcc agaccacttc ctccctggcc aggtcgatgc cgtacacggc
     2941 gccgtccggc agcgacgtgg ccatgtacgc caccgtcccc accacgaacg gccgcccgct
     3001 caccgcacca tccagccgct tcttccaccg tcgttcgccg ctgtcgggcc ggtaacgggt
     3061 gaacaaccgg tcggtcgtcg ccacgaagag ctcattgcca tacagggtcg gcgtgacatc
     3121 gaccccgcgc ccggcatccg ccgtccagag cacccgggga ggcagtcccg tgatcggtgt
     3181 cggcggcttg atggcctcgc cccgggtgta ggtgccgcag cccggggtca tggcaagaag
     3241 gaggagggga gcgagcggaa ggaccgacac gctcatcgca cccaaccggt cgcgcaacag
     3301 gaggaaccgc ccgagaatca gggtcatcca cgcacgcatg gcgcggacct gtatccgcat
     3361 cagtagttcg tcccgatgaa gaaaatcacg tcggta
//
LOCUS       DASVOD010000397         8365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_339174, whole genome shotgun sequence.
ACCESSION   DASVOD010000397 DASVOD010000000
VERSION     DASVOD010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339174"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /locus_tag="VF720_09080"
     CDS             <1..1363
                     /locus_tag="VF720_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7879551.1"
                     /translation="ASVFGGGASFETYASGKYAAALDPGSWTLDFSKFGYFPASAATI
                     AAAGSDVTLDRQLVRRPSGELSGTITGGLANTPLVDARLHLHDTPLATLSVAGGSYDF
                     PDVPEDTWQLGAHRPGYQSKNRVVTITGGNTTTADLHLAPLDFYDNAETPTGWTLFTT
                     GDNATTSGRWVQADPVGTGPQTAPAMVPLRDVFRTQHHPEYEEGPTSGQPEDDSTPAP
                     GTICFITGNGVPGGSTGAADVDNGKTTLTTPLLNLSGRTDPHVTFYYWFVNDVGANAG
                     EDPFDIAISNDGGTNWVPVASLLRATHAWEEYDFRVLDFVTLTTTMKVRFVAQDLGGG
                     SLVEAGIDDFGWYDPLPQSGTPIDGGGPPVAAVLTAGPNPFLLAADLHLERAAAGPLA
                     VTIFDPAGRRVRVLHDGVAPAAWSTRWDGRDDAGTAVPPGVYFVRAVGADVERSLRIV
                     RLR"
     gene            1360..2148
                     /locus_tag="VF720_09085"
     CDS             1360..2148
                     /locus_tag="VF720_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879552.1"
                     /translation="MTSRSRLNRRQFVSVGLTLSILWIGAGPAAIARAQAVLPPVTPA
                     TPIAPLDGVRDPVFELLLAFNLGWPAGHLSGAELIRRVEASGRKSPIPYRGVVWQRRT
                     PLADSSGTSVETVFDRPIDLPVPYEVLGYHPGSFRTSGRFVFREERLGDLVLSDDRTK
                     VDKPLEAVSLDDMHLFVLTEGSLEIDIDGWLDKLAGGNLDDTSIQAMAVFVRDGKRYG
                     LALGKNPDGEGRSGVFDFGTEKVLYPTPEDFKIVSRYLRRELKR"
     gene            complement(2121..2696)
                     /locus_tag="VF720_09090"
     CDS             complement(2121..2696)
                     /locus_tag="VF720_09090"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEX7879553.1"
                     /translation="MDTGARHDTLPRVGVSACLIGWEVRYAGGHKWNALIDDEMGPGV
                     EWIPVCPELEIGLGVPREAIELVGDPENPELVGTESGRRLAATMHAWSARRLEELAAH
                     DLCGYVLKARSPSCGPRNVPVYSSEKSASEVKGPAGANPVPTGIGLFARALLLRFPGM
                     PIVDETELTSREAVSNFVARVYDYRLSSRRR"
     gene            complement(2735..3778)
                     /gene="cydB"
                     /locus_tag="VF720_09095"
     CDS             complement(2735..3778)
                     /gene="cydB"
                     /locus_tag="VF720_09095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005035737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX7879554.1"
                     /translation="MLELWYGIVAVMLTAYVVLDGYDLGSGALHLFLARGDRERRQVL
                     AAIGPYWDANEVWLLATGGALFVGFPRVLASGLSGFYFAIFLVLWCLILRGISVEFRS
                     HVSDRHWRAFWDVGFGIASILLAVFFGAALGNLIRGLPLNAEGWFGLTLFTDFTARPP
                     VGILDWYTVSAGVFALLVLAWHGANWLSWKTDGAVHERAESFGTWLTRLVTLLWPLMT
                     WATASVNADLFRNFPGRPIAWIGLILALFGMLLAWRRKPAGGVGPFLGSCAFITGLMV
                     GTAACVWPVMLRAVPDAAASVTAHTGGNDAAGLSVAIRWWMLGFPIAIAYFVVLFRLH
                     RGKARAAADGEGY"
     gene            complement(3771..5144)
                     /locus_tag="VF720_09100"
     CDS             complement(3771..5144)
                     /locus_tag="VF720_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEX7879555.1"
                     /translation="MPDPSDALVWHRLQFAFTIIFHYLFPALTMGLALLIAVMKWRYL
                     RTNDPAWNEAARFWIRIFGVNFAVGVVTGVPMEFQFGTNWANFSRYAGKVIGHTLGME
                     GLFAFFLESSFLALLVFGEKRVGKVGHFWCAVALFVGSWLSGYFIVATNAFMQHPVGY
                     AIADDGTLRLQDFGAFLLNPWAVAQYAHNMMSTVSVAAFVVAAVGAWYALRGEHLAAA
                     GRFLRVGVVAGLVSSLLVAFPTGDHQAKLVARHQPASLAAMEGRFESGPNAGVAVIGQ
                     PNVREKRLDNPIELPGMLSFLAFGTFHANVPGLNEFPENDWPDNIELLYYAFHVMAGI
                     GTLMIGLMLVATVWLVRGRLLGARPLLWALLLSVPFPYIATIFGWMTAELGRQPWLVY
                     GLFRTAHGASPTVHSGTALFTLIGFCGIYFILGLLFLFLVGRELAHGPAGGHGAGAAG
                     TGEAAHA"
     gene            5338..7062
                     /locus_tag="VF720_09105"
     CDS             5338..7062
                     /locus_tag="VF720_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879556.1"
                     /translation="MSRWNDRARIALTPLRTSLATALFPTLIAIQVHAAPNPDLQTVR
                     TRLDRARAAEDAGRFDEALAAWDSLATANPYDGNLQEGIARARLARHDAPGAVAAWLK
                     ARELGSTPRPQADLEIARCWMVGGQPDSAMAWIETALADGYEQRAALYEDGDFSSIRE
                     SDRFKRLAGRPVGPAPDRNAAWPGDLDFVVAEIKRLHYVYSKAPLPAPLESDIAALRR
                     DIPKLTDDAVFARFERIMAGLGDGHSVLYPVSDVSGPLQRLPLATYFFADGWYVVGAP
                     PELAGRVGSRIESVGGVPTDVLLARLESYMPRDNDMGMLWTGPLMLSFPAMLGVLGAA
                     PDPKAVAVTLVASDGKNETVTLTPGTAEGHMHVPKLAAPATLGVSPPRWLTRVNDFYW
                     FEDMPDLKALYVQCNQVVDKDDESMRDFALRLRKHIDETHPKHLVIDLRHNNGGNSDR
                     LIPFIRTAVHFETTTPGGRTWVLIGRNTFSAAQVLANNLDRLTNAMFLGEPSGSSPNF
                     VGEDTGLRLPNSGYYGSISSRFHQTWNNDDRPWIAPDFAIPLTSGDYFAGRDPVMEAL
                     EDYLRTMP"
     gene            7189..7530
                     /locus_tag="VF720_09110"
                     /pseudo
     CDS             7189..7530
                     /locus_tag="VF720_09110"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7622..>8365)
                     /locus_tag="VF720_09115"
     CDS             complement(7622..>8365)
                     /locus_tag="VF720_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7879557.1"
                     /translation="ESNPVLAVAYPGLVEAVRTISTPQLRNMGTIGGNLCLDTRCNYY
                     DQNYEWRKGINFCMKKDGSVCWVALSSPKCLAVSSTDAVPVLCAMDARFTLVSTAGER
                     TVRAEEMYNNDGINYLHKQPGELITAIHLPATNGWRATYKKLRRRGAFDFPVLGVGAR
                     VKFANDARTLHVESARIFIGGVASAPIRCLEAEALLEGKLMTDETIAAAAEEAARPAR
                     PMDNTDHDRLWRKQMVSVWVKRALEAVRG"
BASE COUNT         1434 a         2949 c         2754 g         1228 t
ORIGIN      
        1 ggcgagcgtg ttcggagggg gagccagttt cgaaacctac gcctccggga agtacgccgc
       61 cgccctcgat ccagggagct ggacgctcga tttctcgaag ttcggctact tcccggcttc
      121 cgcggccacg atcgcggcgg ccggctcgga tgtcacgctg gaccgccagc tggtccgccg
      181 tccctccgga gagctgagtg gcacgatcac gggcggcctg gccaacaccc cactcgtcga
      241 tgcgcgcctt cacctccacg acacgccgct ggccacgctc agcgtggccg ggggcagcta
      301 cgattttccc gacgtccccg aggatacctg gcagcttggg gcgcaccggc cgggctacca
      361 gtcgaaaaac cgcgtcgtga cgatcacggg cgggaatacc accacggccg acctccacct
      421 ggcgcccctc gacttctacg acaacgccga gacgcccacc ggctggaccc tcttcaccac
      481 cggcgacaac gccacgacat ccggccgctg ggtgcaggcc gacccggtcg gcacgggccc
      541 ccagaccgcg ccggccatgg tgccgctgcg ggatgtcttc cgcacgcagc accatcccga
      601 gtacgaggaa gggccgacga gcggccagcc cgaggacgac tccacgccgg ctccgggaac
      661 gatctgtttc atcaccggca acggcgtgcc gggtggctcg accggcgcgg ccgatgtgga
      721 taacgggaag accacgctca ccacgccgct gctcaacctg tcggggcgca ccgatccgca
      781 tgtcaccttc tactactggt tcgtgaacga tgtgggggcc aacgccggtg aggatccctt
      841 cgacatcgcc atttcgaacg acggcgggac gaactgggtg ccggtggcct ccctcctccg
      901 tgccacccac gcctgggagg agtacgactt ccgcgtactc gatttcgtga cgctgaccac
      961 caccatgaag gtgcgcttcg tggcccagga cctcgggggc ggatcgctgg tggaggccgg
     1021 catcgacgac ttcgggtggt acgaccccct gccgcagagc ggcacgccga tcgatggcgg
     1081 tggcccgccc gttgccgcgg tgctcacggc aggtccaaat ccgttcctcc tggcagccga
     1141 ccttcacctc gaacgcgccg ccgccggtcc ccttgcggtc accatcttcg acccggcggg
     1201 gcgacgcgtg cgcgtgctgc acgacggcgt ggccccggct gcctggtcca cccgctggga
     1261 tggtcgcgac gacgcgggca ccgcggtgcc gccgggcgtc tacttcgtgc gcgccgtcgg
     1321 cgcggacgtc gagcgatcgc tccggatcgt ccgcctccgt tgacgagtcg gtcgcgcctc
     1381 aaccggcgcc aattcgtttc ggtcggactt accctgtcga tcctctggat cggggccggc
     1441 ccggccgcga tcgcgcgcgc gcaggccgtc ctcccccccg tcacgccggc cacgccgatc
     1501 gccccgcttg acggtgtccg cgacccggtt ttcgaactgc tgcttgcctt caatcttgga
     1561 tggccggcgg ggcacttgag cggtgccgaa ctgatccggc gggtggaggc ctcgggacgc
     1621 aagtccccca tcccgtatcg gggcgtggtg tggcagcgcc gaacgccgct ggccgactcg
     1681 agcggcacca gcgtcgagac ggtgttcgat cgcccgatcg acctgccggt gccgtacgag
     1741 gtgctcggct atcaccccgg atcgttccgc accagcggcc gcttcgtctt ccgcgaggag
     1801 cgcctgggcg atctcgtcct ttcggacgac cggacgaagg tggacaagcc gctcgaggcg
     1861 gtgtcgctcg acgacatgca ccttttcgtc ctcaccgagg gctccctcga gatcgacatc
     1921 gacggctggc tcgacaagct ggccggcggg aatctcgacg acacctcgat ccaggcgatg
     1981 gccgtcttcg tgcgcgacgg gaagcgctat ggcctggcgc tggggaagaa cccggacggc
     2041 gaggggcgat cgggcgtctt cgatttcggc acggaaaagg tgctctaccc gacgcccgag
     2101 gacttcaaga tcgtgtcgcg ctacctgcgg cgggagctca aacggtagtc gtacacgcgt
     2161 gccacgaagt tcgacacggc ctcgcgcgag gtgagttcgg tttcatcgac gatcggcatt
     2221 cccggaaagc gcaggaggag cgcccgggca aagaggccga tgccggtcgg caccggattc
     2281 gctcccgcgg ggccttttac ttccgacgcc gacttctccg atgagtagac cggcacgttg
     2341 cgcggcccgc acgacggcga ccgcgccttg agcacgtagc cgcaaagatc gtgcgcggcg
     2401 agctcctcca gccggcgcgc gctccacgcg tgcatcgtgg ccgccagccg tcgccccgat
     2461 tcggtgccga cgagctcggg gttttcggga tcgcccacca gctcgatggc ctcccgcggc
     2521 acgccgagtc cgatctcgag ctcgggacag accgggatcc actccacccc cggccccatc
     2581 tcgtcgtcga tcagcgcatt ccacttgtgg ccgccggcgt agcgcacctc ccagccgatc
     2641 aggcaggcgg agacgccgac gcggggaagg gtgtcatgtc gtgctcccgt atccatcgcg
     2701 ggattctgcg ccgatcgaca ccccgcgtcg agcgctagta cccctccccg tccgccgccg
     2761 cccgcgcctt gccgcggtgg agccggaaca aaacgacgaa gtaggcgatg gcgatcggga
     2821 agccgagcat ccaccagcgg atggccacac tcaggcccgc cgcgtcgttg cctccagtgt
     2881 gcgcggtgac ggaggcggcg gcgtcgggca cggcgcggag catcacgggc cagacgcagg
     2941 cggcggtgcc gaccatgagg ccggtgatga aggcgcacga gccgaggaac ggccccacgc
     3001 cgccggcggg cttgcggcgc caggccagca gcattccgaa gagcgccagg atgaggccga
     3061 tccaggcgat cggccgcccg gggaaattgc ggaagaggtc ggcgttcacc gacgcggtgg
     3121 cccatgtcat cagcggccag agcagcgtca ccagtcgtgt gagccaggtg ccgaacgact
     3181 cggcccgttc atgcacggcg ccgtcggtct tccacgacag ccagttggcg ccgtgccagg
     3241 ccagcacgag cagcgcgaag acgccggccg acacggtgta ccagtcgagg atgccgaccg
     3301 ggggtcgcgc cgtgaagtcg gtgaagagtg tgaggccgaa ccatccctcc gcgttcagcg
     3361 gcaacccgcg gatcaggttg cccagcgccg cgccgaagaa gaccgccagg aggatgctgg
     3421 cgatgccgaa gccgacgtcc cagaaggcgc gccagtggcg gtcggacacg tggctccgaa
     3481 attccaccga gatgccgcgc aggatgagac accagagcac gaggaagatg gcgaagtaga
     3541 agcccgacag gcccgacgcg agcacgcgcg ggaaccccac gaacagggcg ccgccggtgg
     3601 cgaggagcca cacctcgttg gcgtcccagt acgggccgat ggccgccagc acctggcgac
     3661 gttcacggtc gccgcgcgcc aggaagaggt ggagggcgcc cgatcccagg tcgtagccgt
     3721 cgagaacgac gtaggcggtg agcatcaccg ccacgattcc gtaccagagc tcaagcatgc
     3781 gcggcctccc ccgttccggc cgccccggcg ccgtgtccgc cagccggccc gtgcgccagc
     3841 tcgcgcccca ccaggaagag gaagaggagc cccaggatga aatagatgcc gcagaagccg
     3901 atgagcgtga agagcgcggt gcccgagtgc acggtgggac tggcgccgtg cgcggtgcgg
     3961 aagagcccgt acacgagcca cggctggcgg ccgagctcgg ccgtcatcca tccgaagatg
     4021 gtggcgatgt acgggaacgg cacgctgagg aggagcgccc acaggagcgg ccgcgcgccg
     4081 aggagccggc cgcgcaccag ccatacggtg gccacgagca tgaggccgat catcagcgtg
     4141 ccgatccccg ccatcacgtg gaaggcgtag tagagcagct cgatgttgtc gggccagtcg
     4201 ttctcgggaa actcgttcag gccgggaacg ttggcgtgga atgtgccgaa ggccagaaaa
     4261 ctgagcatcc ccggcagttc gatcggattg tcgagccgct tctcccgcac gttcggttgc
     4321 ccgatgacgg ccacgccggc gttcggtccc gactcgaacc gcccttccat ggcggccagg
     4381 ctggccggct ggtgcctggc caccagcttg gcctggtgat cgccggtcgg aaatgccacc
     4441 aggaggctcg agacgagccc ggccacgacg cccacgcgga ggaaccgccc ggccgccgcc
     4501 aaatgctcac cgcgcagcgc gtaccaggcg cccaccgccg ccacgacgaa tgcggccacc
     4561 gacacggtcg acatcatgtt gtgcgcgtac tgggccacgg cccaggggtt cagcaggaag
     4621 gcgccgaagt cctggaggcg cagcgtgccg tcgtccgcaa tcgcataccc cacggggtgc
     4681 tgcatgaacg cgttcgtggc cacgatgaag tagcccgaca gccacgatcc gacgaagagg
     4741 gccacggcgc accagaagtg ccccactttt cccactcgct tttcgccaaa gacgaggagc
     4801 gccaggaaac tcgactcgag gaagaaggca aacaaccctt ccatcccaag cgtgtggcca
     4861 atcacctttc cggcgtagcg cgagaagttg gcccagttgg tgccgaactg gaattccatc
     4921 ggcacgccgg tgacgacgcc caccgcgaaa ttgaccccga agatccggat ccagaagcgg
     4981 gccgcctcgt tccaggccgg gtcgttcgtg cggaggtagc gccacttcat cacggcgatg
     5041 aggagcgcca gacccatggt cagggccgga aagagatagt ggaagatgat cgtgaaggcg
     5101 aactggagac gatgccagac gagcgcgtcg cttggatcgg gcaaacaaac ctccggacgg
     5161 atggaccggg ggccggtcgg atcccccggg cgatggcgca cccgcagtct aagaccgctc
     5221 ccgggaccga caaagcccgt ggcgcaacgg ggaccgacat ttgtcgccct ggacgccgaa
     5281 ctccgtgcaa cctggatcga aaacaggccg tcacgggggc ccggaaagga gtgccccatg
     5341 agccgatgga atgaccgcgc ccggatcgcc ctcactcccc ttcgcaccag ccttgccact
     5401 gccctcttcc cgaccctgat cgccatacag gtccacgccg ccccgaaccc tgatctgcaa
     5461 acagtccgca cccgcctgga ccgggcccgg gccgccgagg acgccggccg tttcgatgag
     5521 gccctggccg cctgggactc gctcgccacc gccaacccgt acgacggcaa cctccaggag
     5581 gggatcgcac gggcccggct cgcccgacat gacgcccccg gcgccgtggc cgcctggctc
     5641 aaagccaggg agctcggctc cacgccacgc ccccaggccg acctcgagat cgcccggtgc
     5701 tggatggtgg ggggccaacc cgacagcgcc atggcctgga tcgagacggc cctggccgac
     5761 gggtacgagc aacgggccgc gctgtacgag gacggggatt tttcctcgat cagggaaagt
     5821 gaccggttca aacggctggc gggacgtccg gtcggccccg cgccggatcg caacgccgcc
     5881 tggcccggcg atctcgactt cgtggtggcc gaaatcaagc gccttcatta cgtctactcg
     5941 aaagccccgc tgccggcccc gctcgagagc gacatcgccg cactccgccg cgacatcccg
     6001 aaactcaccg acgatgccgt cttcgcccgc ttcgaacgga tcatggccgg cctcggcgac
     6061 ggtcactcgg tgctgtaccc ggtgagcgac gtgagcggcc cgcttcagcg gctgcccctg
     6121 gccacatact tctttgccga cggctggtac gtggtcggcg ccccgcccga actggccggc
     6181 cgtgtcggca gccgcatcga atcggtgggc ggtgtgccga ccgacgtgct cctcgcgcgc
     6241 ctcgagtcct acatgccgcg cgacaacgac atggggatgc tctggaccgg gccgctgatg
     6301 ctctcgtttc ccgccatgct gggtgtcctc ggcgcggcgc ccgatccgaa ggccgtggcc
     6361 gtcacgctcg tggcatccga cggcaagaac gaaaccgtga cgctgacccc cggcacggcg
     6421 gaagggcaca tgcacgtgcc gaagctggcc gcccccgcca cgctcggcgt gtcgcctccc
     6481 cgctggctca cgcgagtgaa cgacttctac tggttcgagg acatgcccga cctcaaggct
     6541 ctctacgtcc agtgcaacca ggtcgtcgac aaggacgacg aatcgatgcg tgacttcgca
     6601 ctgcgcctgc ggaagcacat cgacgagacg cacccgaagc atctcgtcat cgacctgcgc
     6661 cacaacaacg gcggcaacag cgaccggctg atcccgttca tccgtacggc cgtccacttc
     6721 gagacgacca cacccggcgg caggacgtgg gtgctgatcg gccgcaacac cttcagcgcc
     6781 gcccaggtgc tggccaacaa cctcgaccgc ctgaccaacg cgatgttcct cggcgaaccg
     6841 tcggggtcga gccccaactt cgttggcgag gacaccggcc tccgcctccc gaacagcggc
     6901 tactacggca gcatctccag ccgctttcac cagacgtgga acaacgatga ccgaccctgg
     6961 atcgcgccgg acttcgcgat tccgctgacg tccggcgact atttcgcggg gcgtgatccg
     7021 gtgatggagg cgctggagga ttacctccgg accatgcctt agggcggtac tgccgatgtc
     7081 gaagggcaag atatggatgg ccgcgccatc gcgcggcctc tccactcaga actccgactg
     7141 gacagacctc gaggcccgac ctaggctcac gtcatgacca tcacgcgaat ggaccacgtc
     7201 agcatcatcg tcgacgacct gccggccgcc atcgactttt ttgccaccgt cggcatggcc
     7261 atggtgggcg aggcggccgt cgaggggccg tgggtcgacc agctcaatgg gctcgaaggc
     7321 gtgcaggtga acatcgccat gatgcgcacg ccggacggcc acggccaggt cgaactgacc
     7381 agcttccgca acccgaagct cgtcgactcc acgccggaaa atgcgccgcc caacacgctg
     7441 ggccttcgcc aggtgatgtt cggcgtggac aacatcgacg acaccgtggc gcgtctcgcc
     7501 tcgctcggct acgggctgat cggcgagatg gtcgagtaca tcgggaagta tcggctgtgc
     7561 tatgtgcggg ggccggcggg gatcatcgtg gcgctggcgc aggagttggg gctgagcgag
     7621 gctacccccg caccgcctcc aacgcccgct tcacccagac cgacaccatc tgcttccgcc
     7681 acaaccggtc gtggtcggtg ttgtccatcg gacgcgccgg acgcgcggct tcctcggcgg
     7741 cggcggcaat ggtctcgtcg gtcatcagct tcccttcgag cagcgcctcc gcctccaggc
     7801 accggatcgg tgccgaagcc acgcccccga tgaagatccg cgccgactcc acgtgcagcg
     7861 tcctggcgtc gttcgcgaac ttgacccggg cgccgacacc cagcaccgga aagtcgaacg
     7921 ccccgcgacg acgcagcttc ttgtaggtgg cccgccagcc gttggtggcc ggcaggtgga
     7981 tggcggtgat caattcgccc ggctgcttgt gcaggtaatt gatgccgtcg ttgttgtaca
     8041 tctcctcggc gcgcaccgtg cgctcacccg ccgtcgacac gagcgtgaag cgggcatcca
     8101 tggcacagag gaccgggacg gcatccgtgg aactgaccgc caggcacttg ggactcgaca
     8161 gcgccaccca gcagacgctg ccgtccttct tcatgcagaa gttgatcccc ttccgccact
     8221 cgtaattctg gtcatagtag ttgcagcggg tgtcgaggca gaggttgccg ccgatcgtcc
     8281 ccatgttgcg gagctgcggc gtggagatcg tgcgcaccgc ctcgacgagc ccgggatacg
     8341 ccactgccag caccgggttc gattc
//
LOCUS       DASVOD010000398         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_861933, whole genome shotgun sequence.
ACCESSION   DASVOD010000398 DASVOD010000000
VERSION     DASVOD010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861933"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1813
                     /locus_tag="VF720_09120"
     CDS             161..1813
                     /locus_tag="VF720_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7879558.1"
                     /translation="MKRISKSTVATLALALAAGAFLGIVLTGDRVQAQTEKGLYSQLN
                     LFSRVLDLIQTNYVEEVDPQPLIQGAITGMLAELDPHSTYVDSERFAKMNERNQGEYH
                     GIGVSFEIRDNYITVISPIEGSPSDALGIRGGDRIIKIDGESAKGISSEEVFDKLRGP
                     KGSKVHVTIQRPGIDELFEYDIIRDKIPIYSVPYHFMLDDQTGYVRAIRFSATTAQEL
                     SEAIHDLLDKGMQRMVFDLRGNSGGYLNQAIEVSDMFLPGGKTVVYTKGRIPDSSQYY
                     YSTDNDKLPNFPVIVLVDHGSASASEIVSGAIQDHDRGLILGMTTFGKGLVQRQYTLK
                     DGSALLLTVARYYTPSGRLIQRDWTDRDKYVSEEAIEAAEAETEKAEAGTEDLPKFKT
                     DSGRTVYGGGGIAPDIRTEYTRKLTELQTRLEREQLFSEFAPKLANGSGMTKADDFEK
                     FVDDWQVNDATLSQFKSFVDDKSKALPDDRKINYTPEEWKTDLDYSRQALKREVARSV
                     WGDMSRYRVAIADDEMIEEAMRHFDQAALMAKNMESNTVEQR"
BASE COUNT          391 a          652 c          562 g          294 t
ORIGIN      
        1 ccggagcgtt cgggcggcgg ggagccgcta accgcgtgat atacttgacg tccggaagat
       61 gccgcgaggc aattcatccg gaaccagaca cccgtttcga caatccaaag cccgtcccgc
      121 cggccgtgcc ggcatcgatc ggatcctcac ggaggttcat ttgaagcgca tctcgaagtc
      181 gaccgtggcg acactggccc tggcactcgc ggccggtgcg ttcctcggta tcgtcctcac
      241 cggcgaccgg gtccaggccc agaccgagaa ggggctctac tcccagctca acctcttcag
      301 ccgggtcctc gacctgatcc agacgaacta cgtggaggaa gtcgatcccc agcccctgat
      361 ccagggcgcc atcaccggaa tgctcgctga gctcgatcct cactccacgt atgtcgattc
      421 cgaacgattc gccaagatga acgagcggaa ccagggcgaa taccacggca tcggcgtctc
      481 cttcgaaatc cgcgacaact acatcacggt catctcgccg atcgagggga gcccgtccga
      541 tgccctcggc atccgtggcg gcgaccggat catcaagatc gacggcgaga gcgccaaggg
      601 gatctcctcg gaggaagtgt tcgacaagct ccgcggcccc aagggctcga aggttcacgt
      661 cacgatccag cgccccggca tcgacgagct cttcgagtac gacatcatcc gcgacaagat
      721 ccccatctac tcggtcccct accacttcat gctcgacgac cagaccggct acgtccgcgc
      781 catccgcttc tcggccacca cggcccagga actcagcgag gccatccacg acctgctcga
      841 caaggggatg cagcggatgg tgttcgacct gcgcggcaac tcgggcggct acctgaacca
      901 ggccatcgag gtgagcgaca tgttcctgcc gggcggcaag acggtcgtct acaccaaggg
      961 tcggattccc gattcgagcc agtactacta ttccaccgac aacgacaagc tccccaactt
     1021 cccggtcatc gtgctggtcg accacggctc ggcttccgcc tcggaaatcg tgtcgggcgc
     1081 catccaggac cacgatcgcg gcctcattct cggcatgacc accttcggca aggggctcgt
     1141 ccagcggcag tacacgctga aggacggctc ggcgctcctc ctcaccgtgg cgcgctatta
     1201 caccccgagc ggccggctca tccagcgtga ctggaccgac cgcgacaagt acgtctccga
     1261 ggaagccatc gaagcggccg aagccgagac cgaaaaggcc gaagcgggca ccgaggatct
     1321 ccccaagttc aagaccgact cgggccgcac cgtgtacggc ggcggcggca tcgcacccga
     1381 catccgcacc gagtacaccc gcaagctcac cgagctgcag acccggctcg agcgcgagca
     1441 gcttttcagc gagttcgcgc cgaagctggc caacggaagc ggcatgacca aggcggatga
     1501 cttcgagaag ttcgtcgacg actggcaggt gaacgacgcc acgctttcgc agttcaagtc
     1561 gttcgtggac gacaagagca aggcgctgcc ggacgatcgc aagatcaact acacgccgga
     1621 agagtggaag accgacctcg actactcccg ccaggcgctg aagcgcgaag tggcccggag
     1681 tgtctggggc gacatgagcc gctaccgggt ggccatcgcc gacgacgaga tgatcgagga
     1741 agccatgcgc cacttcgacc aggctgccct catggccaag aacatggaat cgaacacggt
     1801 cgagcagcgc taggccgttc ctgctcctga gcggggcggg gtcccttacc gggccccgcc
     1861 cctttttttg tgcccggcca gaaagtcgcc cacgaggta
//
LOCUS       DASVOD010000399        14925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_391742, whole genome shotgun sequence.
ACCESSION   DASVOD010000399 DASVOD010000000
VERSION     DASVOD010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391742"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VF720_09125"
     CDS             complement(<1..751)
                     /locus_tag="VF720_09125"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX7879559.1"
                     /translation="MARLRVGVLYEHFWGEDETPTPGKRPRRQDPVKDVVEVHRALKK
                     LGHSPTFIRLDGSVESLQALARRKVDLIFNLTESWAGDDTKDMNIAAYLELLGRPFTG
                     SGSLGLATGADKVMAKKIFEFHKIPTPNFATVWRGRMDHAHDINFPVIVKPAREDGSI
                     GIPFSASVESITELMMRIDELHAEFDTPVLVEEYVEGREIYVGVLGNKKPEALPPIEL
                     DLSKLPEGTPKIAGTEVKWERSTEAYKTTQPR"
     gene            complement(795..1808)
                     /locus_tag="VF720_09130"
     CDS             complement(795..1808)
                     /locus_tag="VF720_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEX7879560.1"
                     /translation="MDRFEHPRTHNADLLSRRISQLGLKLEGSPVERFVRQLYRELER
                     KGLARFRPVCYLTDEWGCPSGEPVIGIPFYLADPELADLEKAMNDLEDEREIMRYLRH
                     EAGHAFNYAYQLYKTPEWRRLFGPWNRPYRDHYRPIPFSRKFVRHLAGWYGQKHPDED
                     FAETFAVWLTPRSNWREKYANWPAIHKLKYVDLVAKKVRNTDPPMPSGDFDITVSDMK
                     LTVEQFYRRVMRQNERAPKLVLDSELKEIFLPKNRLRKNKRAAADLITENRLALIDNI
                     SDWTGVRRPLIKTLVEAMADASRRLELEADPRRAAEYLIEMTAFGTTLAMNYMNRGRF
                     EIR"
     gene            1993..2433
                     /locus_tag="VF720_09135"
     CDS             1993..2433
                     /locus_tag="VF720_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879561.1"
                     /translation="MFPLSTPSALRPLFAMMSLALVALTAGFDLAEFRICLEKDGRVH
                     AELSSSACGLDDGGCPDSGNEPADLPGGECDEDCLDISGTVPALRHEAVTTMALTPGL
                     TVLPPSHPDLLPTTLVAVEAPHRFVADHLNGSTFSFRETIVLTS"
     gene            2534..3808
                     /locus_tag="VF720_09140"
     CDS             2534..3808
                     /locus_tag="VF720_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7879562.1"
                     /translation="MSYRCISIFLLALFAPAWVRAHATDTHAAPGSLSLDEARRRAQA
                     YSPTLAATRIGIDVAAARVNQAGSRPVPELGIEVENFGGTLNGFKRAESTFMLSQRFE
                     RGGKRGARLDAARAVRSLAESEVALDELDLARDVAVAFAEGLAAQERLRVEEEVLGLS
                     ADILRSVEEKVQLGAALRAEATRARVAQSLVQADVDAAREEMETSRRSMARLWGSSVP
                     EFERFVGLLDSLRAVPGGGEIDSLINSNPRLEVADATIRLRDSEVRLEKSAGKRDLSV
                     GAGYRLLDGTEDGAFVAGLSIGLPFGDRNQGNIEAVAASLRAAEAERQVVTEALRRAL
                     DQTTGRIAGAQAAVRRLREDAIPAANDSYREVENAYRQGRLSYIDLLEARRALTDARL
                     REIGWLTQLFTLRAELEWIAGRPLPALWEASR"
     gene            3805..4881
                     /locus_tag="VF720_09145"
     CDS             3805..4881
                     /locus_tag="VF720_09145"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7879563.1"
                     /translation="MTSGDIKVAGATGAILILALALATGWLATGCSGKRSEVASKTGA
                     AEHAEHEKESDDHGGHDHGAEAGHDHGAETEPHAEGHDEHESEAVRLTPQQRDAAGIT
                     IETAGPASIGRPLELPGEIVLDADHLSHIVPRFPGVARTVHKGLGDRVRAGEVLAVLN
                     SNESLSTYDVPSLIDGIVIEKHITLGEFVRDDQDIYVVADLSKVWVNMTVYPRDLARI
                     HTGLPVSIRITGVEGERLGKIGYIGPVVGEGTRTALARVILPNPDGSLRPGLFVTGRV
                     SLDATTVRIAVEDRALQTMDGRTVVFVESAPGEYLAQAVEAGVTDGEWTEVLSGITAG
                     QRYVSGGSFILKSEKLKSEAGHEH"
     gene            4871..8080
                     /locus_tag="VF720_09150"
     CDS             4871..8080
                     /locus_tag="VF720_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX7879564.1"
                     /translation="MSTEDRPTGPKGPLEHLLNFSIGQRGLVLAGALGIAILGVLNFN
                     RLPIDAVPDITNVQVQVNTSVSALSPVEVERQITFPVETAMSGLPRVEQVRSLSRYGL
                     SQVTVIFHDGTDIYWARQLVNERLQEARESLPPGLGQPSMGPISTGLGEIFMWAVTAD
                     STARRPDGQAWSASDLRTLQDWVVKPQIRTVPGVTEVNSIGGYQRQFHINPDPDRLIA
                     HGLSFHDVFEAVARNNADAGAGYIEHREESYLIRTTGRVASFDDLNNIVIGTTDGVPV
                     RIRDVAEVTEGKELRTGAATWLGGEAVIGTSIMLVGENGRSVARRVADRLEAVNRTLP
                     PGVKAVTLYDRTHLVDATLETVRKNLFEGAVLVVAILFLLLGNLRAALIVTLTIPLSM
                     LFSITGMVQNRISGNLMSLGAIDFGIIVDGAVVMTENIVRRLAERQAHLGRTLTKAER
                     LEEAFASSREVARPTLFGVGIIMIVYLPILTLTGIEGKMFRPMAEVVLLALGGALLLT
                     FTFVPAALALVLGGKVRESEPAFLRWTRQGYRRSLDWALGHRQPVLVGAFLILAGSGF
                     LATRLGSEFVPQLQEGSLAIQAIRIPSTSLSTSVAMQGQLEHFLLKRFPHEIESVFAR
                     VGTAEIATDPMGPNIADTYVMLKPIKEWTKAGNQDELARAIGEALAELPGQNYEVSQP
                     IELRFNELISGVRSDLAVKVYGDDLDVMEREANRIARELSAIPGATGVKVEQVSGLPV
                     LTIDVDRGAIARYGLNVEDVQAVVATALGGAEVGQVLEGDRRFDIVVRLPEERRRDIH
                     LLEDLPVPLPVAASGGPLLAGLDPEGMTHASYVPLGSVAHVAIVEGPNLITRENGKRR
                     IAVMANVTGRDIGGFVAEAQGRIERNIQLPPGYWLGWGGQFENLLAARRRLEIVVPVS
                     LVLIFLLLFMTFGSMKHAALVFTGVPLALTGGVMALALRGIPFSIPAGVGFIALSGVA
                     VLNGLVMMTFVNQLREEGLGIDEALRKGAETRLRPILMTALVASLGFVPMALAHGTGA
                     EVQRPLATVVIGGIVSSTILTLIVLPVLYRMAHRQRPEA"
     gene            8258..11512
                     /locus_tag="VF720_09155"
     CDS             8258..11512
                     /locus_tag="VF720_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879565.1"
                     /translation="MLRIRISWLLSPAVLVLIGTQVAARPADPGLEIRPEQANLASDG
                     FSAPLSGLSSHIYTDTVSYGGTYWAPDSLRWEAIRDSHWSFDSGVGSSINGGSNPNKP
                     VGYHQSMEGWFGLDQTLSPLPYFRRSSTCAIAGSFSFWAGVTLAEANALCYAGGQGYG
                     NNWNLIIEKTFAYPGVGTVSFSFQYAVESEAGFDYAYALIDTSGDGSTDDIVLWTKDG
                     VASGTANVALNAGQDMRSTAGNYTIRFVAASDGSYSDEDGLNPSTCGHLAIDNVTVGA
                     DVSNFETGANGWTQTISSTGVGDFSHIADLADLEPPAVFCPCAVRDSVLVFFDTNDQH
                     PLDQDNLAVSPWIDLLANGDAGRSGKLYARDVVAVMPLENYVFLQHLVRYYPAVCSAT
                     GLVYRTPFLNLVSVEYFGPTPICNSVLAPSLRDASATIPPAAEQVQLAVGIINLCRTS
                     PFGPPCTGFNNQTPYLDNLSLGVYGSTTTPVVNTTTFDRFQDNFATDGTLNPASTGRF
                     DQNRIKGGSTPSSGSILGDTLNARGNGGNTEVRLVFRVRSGPFINAGILASYVARWTP
                     EPTLNTRYGGTWYSARMDTAEQAGTPAVSPFAWMGTFHEADPGFSGTDRTVDPNDPNR
                     LANDIIPDHLFTPGSRIDYFVASRYRPPDPRNPGGTAWATDPDTLNATFREAEILPSS
                     MAADSSWNCVLYVDHHDDRSGFDQALEETGLRLSLGGGGANAEGTRHDRFDVQTPSSA
                     QMSFGRPLFSRYGVSLLQLFGFKTVVWHSATLTSGQLTNEDAYILMPWLQLRAIGNNN
                     FWGSGEGLMQSMHNTGSGARFMLNNVLGVVQNCTGIRLANCGGTGIDTTYCIPTSAVA
                     GSHFTSTILPRGRGNGCPDLLSFDRLDVNPSVASAKGQLNYRRLVPGQGGALTDIGFA
                     SVTNLNTIDVSYRTALDGISVGRMRSNAGYFGVGCDDVTASYERTDDVLDWLGGPLNC
                     RVPQALVDVPELLDLPAPAFRHALGNAYPNPMNPTTRIQFTNGTANGRVKVEIFDVTG
                     RLVRSLVDAKMPAGVHEVTWDGGNEAGSSVPSGMYFYRMSAEDFVAAKKLLVSK"
     gene            11719..>14925
                     /locus_tag="VF720_09160"
     CDS             11719..>14925
                     /locus_tag="VF720_09160"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879566.1"
                     /translation="MRDGKALILSLALLLASSGPALARPIDRLSEYRPELSDPSIEAA
                     TSQSPGLSSHIFGDTVSYGGTYWAPDSLRWEAIRDSHWSFDTGVGSSINTGANPNKPV
                     GYHQQMEGWFGLDQTLSPLPYFRRSSTCAISGSFSYWAGVTVPEATALCFAAGQGYGN
                     SWTLTIAKSFAYAGAGNIAFSFQYAVEAEPGFDYAYAMIDTTGNGSQDDLILWTQDGT
                     AAGTASITLNPGQDMRSTAGNYTVKFVAASDGGYADEDGAYATTCGLLAVDNVTVGAD
                     VSDFETGDNGWAQMIPTTGIGDFTNLAYTPDVEAPPTFCPCGVQDSILVFFDPNDGHP
                     IDQDNIAVSPWIDLLAGGDVGKPGKLLLHDVVAIMPLTNYIFTQYRVRYYPEVCAATG
                     LLYRTPWKDQNVVFYWGEQPICNPANAPRLRDYSAVFSPGAQQAQLAFGMISLCRTSP
                     FGPLCTGVTNQTPYYDNISLGVYGNATVPVVNTTTFDRFQDNFAADGTLHPAAPGRFD
                     QNRIKGASTPSTGTILGDTLNARGNGGNTEVRLVFRVRTGPFVNGATLASYVARWTSE
                     PTLGARYGGNWYSARMDTAEQGGVPATSPFTWMSTFHEADPGFIGTDRAADPNDPNRL
                     ANDIIPDHLFTPGSRLDYFVAARYRPPDPRNPGGTDWSTDPDTAGSRFREAEILPSSF
                     AADATWNCLLYVDHHDDRDQDQQYLEEAGLQQSLGAGSTNAENTRYDRFDNQNPSAAQ
                     LSFGRPNGTNYGCSQIQVNFYKSITWQAASMTSGAVTAQDVAILSPWLLLRASANNAF
                     WGSGDGIVQSMHATGGSTRAFMNNTLGVLQNCTGIRLAACGGAGIDTTYCMPLSPVAD
                     AWFASSVIPHGRGNGCPELRAFDRLDRNASAASSKGQLNYVRLVPGTGGVPTDIGFAS
                     VANLTTSSSIYRSVLDGLGVGSLRTNSGGFGTGCDDVSASLARTDDILDWFEAPLRCR
                     YPGLVGTPDPPATPYAPGFRLSLGPAWPNPMTTTTRLQFTNAVPQADVRLTIFDVTGR
                     VVRTLHKGEAAVGTHELAWDRTNDEGRPVAGGIYFYRLA"
BASE COUNT         2482 a         5068 c         4790 g         2585 t
ORIGIN      
        1 ccctcggttg tgtggtcttg taggcctcgg tggagcgctc ccacttgacc tcggtgccgg
       61 cgatcttggg cgttccttcc ggcagcttgg agagatcaag ctcgatcggc ggcagcgctt
      121 cgggcttctt gttgccgagc acgcccacat agatctcgcg cccctccacg tactcctcca
      181 cgagcacggg ggtgtcgaac tcggcgtgga gctcgtcgat gcgcatcatc agttccgtga
      241 tgctctcgac ggaggccgag aaggggattc cgatcgagcc gtcctcgcgg gccggtttca
      301 cgatgacggg aaagttgatg tcgtgggcgt ggtccatccg gcctcgccag acggtggcga
      361 agttgggggt ggggatcttg tggaattcga agatcttctt ggccatcacc ttgtccgcgc
      421 cggtggcgag gcccaggctc cccgaaccgg tgaagggacg ccccaggagc tccaggtaag
      481 ccgcgatgtt catgtccttg gtgtcgtcgc cggcccagga ttcggtgaga ttgaagatga
      541 ggtccacctt gcggcgggcc agcgcctgaa ggctctccac cgagccgtcg aggcggatga
      601 acgtggggct gtggccgagt ttcttcagcg ccctgtgcac ctccacgaca tccttgaccg
      661 gatcctggcg tcgcgggcgc tttccgggcg tgggcgtttc atcctcgccc cagaagtgtt
      721 cgtacagaac tccgactcgc aggcgtgcca tcagtgttcc ttcgcccggg gtggcggcgg
      781 gagaggactc ggcgtcatcg gatttcgaag cgcccgcggt tcatgtagtt catggccagc
      841 gtggtgccga aggccgtcat ctcgatcagg tactcggcag cccggcgggg atcggcttcc
      901 agttcgagcc gtcggctggc atcggccatg gcctccacca gggtcttgat gagggggcgg
      961 cgaacgccgg tccagtcgct gatgttgtcg atgagcgcca ggcggttctc cgtgatcagg
     1021 tcggcggcgg cgcgcttgtt cttgcgcagc cggttcttgg ggagaaaaat ctccttcagt
     1081 tccgaatcga gcaccagctt cggggcgcgc tcgttctgtc gcatcacccg ccggtaaaac
     1141 tgctccaccg tcagcttcat gtccgacacc gtgatgtcga aatcgccgct cggcatcggc
     1201 ggatcggtgt ttcgcacttt tttcgcgacc aggtccacgt acttgagctt gtggatggcg
     1261 ggccagttgg cgtacttctc ccgccagttc gagcggggcg tgagccacac ggcaaacgtt
     1321 tcggcgaagt cctcgtcggg atgcttctgg ccgtaccagc cggccaggtg gcgcacgaac
     1381 ttccggctga acgggatcgg gcgatagtgg tcccggtagg ggcggttcca cggaccgaag
     1441 aggcgccgcc actcgggggt cttgtagagc tggtaggcgt agttgaaggc gtgccccgcc
     1501 tcgtgccgca ggtagcgcat gatctcgcgt tcgtcctcga gatcgttcat ggccttttcg
     1561 aggtcggcca gttcggggtc ggccaggtag aacgggatgc cgatgaccgg ctcgccggag
     1621 gggcaccccc actcgtcggt gaggtaacag accggccgga atctggccag gcccttccgt
     1681 tccagttcgc gatacagctg ccggacgaag cgctcgacgg gggatccctc gagcttgagt
     1741 ccgagctggc tgatgcgccg cgacaacagg tcggcgttgt gcgtgcgcgg atgctcgaag
     1801 cggtccacgg cgggcaatct aacgcccaaa tcacgattcc gcaagcggtt gccaagggtt
     1861 gaccggtcgg aagcggccgg gcaggccgct cttcccgtca agcccgcgcg ggaaccgccg
     1921 ataacgaaga ggagttcggc gtcatcccgg gaccgttgac agcaccctca tcccaacgga
     1981 cactcatcga ccatgtttcc cctctccaca ccttcggccc tgcgaccgct cttcgcgatg
     2041 atgtcgctgg ccctcgtcgc cctgacggcc ggcttcgacc tggccgaatt ccggatctgc
     2101 ctggaaaagg acggacgggt ccacgccgaa ttgtcctcga gcgcgtgcgg ccttgacgat
     2161 ggcggttgcc cggactccgg caatgaaccg gccgaccttc cgggagggga gtgcgacgaa
     2221 gactgcctcg acatctcggg gacggtcccg gcccttcgcc acgaagccgt cacgacgatg
     2281 gcccttacgc cgggacttac cgtcctccct ccgtcgcatc ccgatctcct cccaacgacg
     2341 ctcgttgcgg tcgaggcgcc ccatcggttc gtcgccgacc acctgaacgg cagcacgttc
     2401 tccttccggg aaacgatcgt cctcacctcc tgagttcccc gagtctccga acgaccactc
     2461 gctgatcacc gtccggccgg ggcatgcctc cggccgatcc ggccgttcat cctgagacag
     2521 gggaatcacc cgaatgagtt accggtgcat ttcgatattt ctgcttgccc tgttcgcacc
     2581 cgcatgggtg cgcgcccacg caaccgacac tcacgccgcc ccgggtagcc tgagcctgga
     2641 cgaggcacgc cgacgggcac aggcctacag cccgacgctg gccgcgacac ggatcgggat
     2701 cgatgtcgcc gcggcgcgcg tcaaccaggc cggcagccgg ccggtcccgg agctcggcat
     2761 cgaagtcgag aacttcggcg gcacgctcaa cggtttcaag cgcgccgaat ccaccttcat
     2821 gctcagccag cgtttcgaac ggggcggcaa gcgcggcgcc cggttggatg ccgcacgtgc
     2881 cgtccgttcg ctggccgaat ccgaagtggc gctcgacgaa ctcgacctcg cgcgcgacgt
     2941 ggcggtggcc ttcgccgaag ggttggccgc ccaggagcgg ctgcgggtcg aggaggaggt
     3001 gctcgggctc tccgcggaca tcctccgttc cgtcgaggaa aaggtgcagc tcggcgccgc
     3061 actccgggcc gaggccacgc gggcacgcgt ggcgcagagc ctggtccagg ccgacgtgga
     3121 cgcggcgcga gaggagatgg agacgtcgcg gcggagcatg gcccgcctct gggggagcag
     3181 tgttccggag ttcgagcgct tcgtcgggct gctcgacagc ctgcgagcgg ttcccggggg
     3241 gggagagatt gattcgctca tcaacagtaa cccccgcctc gaggtggccg atgccaccat
     3301 ccggctccgt gactcggagg tccgacttga gaagtcggcg gggaagcggg atctctcggt
     3361 cggggcgggc taccgcctgc tcgacggcac cgaggacggc gccttcgtgg ccgggctctc
     3421 gatcggtttg ccgttcggcg acaggaatca aggaaacatc gaagccgtgg ctgcttcact
     3481 ccgcgcggcc gaagcggaac ggcaggtcgt gaccgaagca ctccgtcgtg cgctcgacca
     3541 gacgaccggc cggatcgccg gggcgcaggc ggcggtccga cgcctgcggg aagatgccat
     3601 tcccgccgcc aacgattcgt accgtgaggt tgagaacgcc taccgccagg gacgactgag
     3661 ctacatcgat ctcctggagg cccgccgcgc gctcaccgat gcgcggcttc gtgaaatcgg
     3721 atggctgacg cagctcttta cgctgcgggc cgaactcgaa tggatcgccg gcaggccact
     3781 gcccgcgttg tgggaggcgt cgcgatgacg tccggagaca tcaaggtggc cggcgcgacc
     3841 ggcgccatcc tcatcctggc gctcgccctg gccaccggct ggctggcgac cggttgcagc
     3901 gggaaacgtt cggaggtggc gtcgaagacc ggggccgccg aacacgcgga acacgagaag
     3961 gagagcgacg atcacggcgg ccacgaccat ggcgccgagg ccggccacga ccacggcgcc
     4021 gagaccgagc cgcatgccga aggtcacgac gagcacgaat cggaggccgt ccggctgacg
     4081 ccgcagcagc gcgacgccgc gggaatcacc atcgaaacgg ccgggccagc gtcgatcggg
     4141 cgcccgctcg agcttcccgg cgagatcgtg ctggacgccg atcacctgag ccacatcgtc
     4201 ccacggtttc caggcgtggc ccgcaccgtc cacaagggcc tgggcgaccg ggtgcgggcc
     4261 ggcgaggtgc tggcagtcct gaacagcaac gagagcctgt cgacatacga cgttccctcc
     4321 ctcatcgacg gcatcgtcat cgaaaagcac atcacgctgg gcgaattcgt ccgcgacgac
     4381 caggacatct acgtcgtggc cgacctctcg aaggtgtggg tcaacatgac ggtctatccg
     4441 cgcgacctgg cccggatcca caccgggctg cccgtctcca tccggatcac cggggtggag
     4501 ggggaacggc tcgggaagat cggctacatc gggccggttg tcggagaagg gacgcggacc
     4561 gcgttggcgc gggtgatcct gccgaatccg gatggatcgc tgcggcccgg gctcttcgtg
     4621 acggggcgtg tgtcgctcga tgccacgacc gtgcgcattg ccgtggaaga ccgggccctc
     4681 cagacgatgg atggcaggac cgtcgtcttc gtcgagtcag cgccgggcga gtacctggcg
     4741 caggccgtgg aggccggtgt caccgacggt gagtggaccg aggtcctctc cggaatcacg
     4801 gcgggccagc gctacgtgag tgggggttcg ttcatcctga agtcggaaaa gctgaagtcg
     4861 gaggccggcc atgagcactg aggaccggcc caccggcccg aaagggcccc tggaacacct
     4921 gctcaacttc tcgatcggac agcgcgggct cgtgctggcc ggcgccctcg ggatcgcgat
     4981 cctcggcgtg ctcaacttca accgtcttcc gatcgatgcc gtgccggaca tcaccaatgt
     5041 ccaggtgcag gtcaacacct cggtatcggc gctctcgccc gtggaagtcg agcgacagat
     5101 caccttccct gtggaaaccg ccatgtcggg gctccccagg gtggagcagg ttcgctccct
     5161 ctcccgctac ggcctctcgc aggtcacggt gatctttcac gacggcaccg acatctactg
     5221 ggcccggcag ctcgtcaacg agcggttgca ggaagcccgc gagagccttc cgcccggcct
     5281 cgggcagcct tcgatggggc cgatctccac cggcctcggc gaaatcttca tgtgggcggt
     5341 gaccgccgat tccaccgcgc gccgccccga tggccaggcc tggagcgcgt cggacctgcg
     5401 cacgctgcag gactgggtcg tcaagcccca gatccgcacg gtacccggcg tgaccgaggt
     5461 caactcgatc ggcgggtacc agcgacagtt ccacatcaac cccgacccgg accggctgat
     5521 cgcccacggg ctgtcgttcc acgacgtctt cgaggccgtg gcgcggaaca acgccgacgc
     5581 gggggcgggc tacatcgagc accgcgagga gtcgtacctg atccgcacga ccggacgcgt
     5641 ggcctcgttc gatgacctga acaatatcgt catcggcacc accgacggcg tgccggtgcg
     5701 catccgcgac gtggccgagg tcaccgaagg gaaggagctc cggaccggcg ccgccacctg
     5761 gctgggcggc gaggcggtca tcggcacgtc gatcatgctg gtcggcgaaa acggccgctc
     5821 cgtggcgcgg cgcgtggccg accggctgga agcggtgaac cgcacgctgc cgcccggggt
     5881 caaggccgtc acgctctatg accgcacgca tctcgtggat gccacgctcg agaccgtccg
     5941 gaaaaacctc tttgagggag ccgtgctcgt cgtggccatc cttttcctcc tccttggcaa
     6001 cctgcgtgcc gccctgatcg tgacgctcac gattccgttg tcgatgctct tctcgatcac
     6061 cgggatggtg cagaaccgga tcagcggcaa cttgatgagc cttggcgcga tcgacttcgg
     6121 catcatcgtc gacggtgccg tcgtgatgac cgaaaacatc gtgcgtcgtc tggccgagcg
     6181 acaggcacac ctggggagga cgctcacgaa ggcggaacga cttgaggagg ccttcgcctc
     6241 gtcacgggaa gtggcgcgcc cgacgctgtt cggcgtgggg atcatcatga tcgtctacct
     6301 cccgatcctc acgctcaccg gcatcgaagg gaagatgttc cggccgatgg cggaggtggt
     6361 gctgctggca ctgggaggcg cgcttctcct cacgttcact ttcgtgccgg ccgcgctggc
     6421 gctcgtgctc ggcggcaagg tccgcgagtc cgagccggca ttccttcgct ggacccgcca
     6481 gggctaccgt cgctcgctcg actgggcgct tggccatcgc cagccggtcc tggtcggggc
     6541 attcctcatc ctggcggggt cgggctttct ggccacgcgg ctcgggagcg agttcgtgcc
     6601 gcagctccag gaggggagcc tggccatcca ggccatccgg attccgtcca cgagcctctc
     6661 gacgagcgtg gcgatgcagg gacagctcga gcatttcctg ttgaagcgat tcccgcacga
     6721 gatcgaatcg gtcttcgcgc gggtgggaac ggcggagatc gccaccgacc cgatggggcc
     6781 gaacatcgcg gatacctacg tgatgctgaa accaatcaag gaatggacga aggctgggaa
     6841 ccaggacgaa ctggcgcggg ccatcgggga ggcgctcgcc gaactccccg ggcagaacta
     6901 cgaggtgtcg caaccgatcg aactccggtt caacgagctg atttccggtg tgcgaagcga
     6961 cctggcggtc aaggtgtacg gcgacgatct cgacgtcatg gagcgcgagg ccaaccggat
     7021 cgccagggag ttgtcggcca ttcccggcgc caccggcgtg aaggtcgagc aggtctcggg
     7081 ccttccggtc ctcacgatcg acgtcgaccg gggcgccatc gcgcgctacg ggctcaacgt
     7141 cgaggacgtg caggcggtcg tggccacggc gctcggcgga gccgaagtgg ggcaggtcct
     7201 cgaaggggat cggcgattcg acatcgtcgt gcgcctcccc gaggagcgcc ggcgcgacat
     7261 ccacctactc gaggatctgc cggtgccgct cccggtggcc gcttcgggcg ggcccctcct
     7321 ggcgggcctc gatccggagg ggatgactca cgcgtcgtac gtgccactcg gttcggtggc
     7381 ccacgtggcc atcgtcgagg gcccgaacct gatcacgcgc gagaacggca agcggcggat
     7441 cgccgtcatg gccaacgtga ccggtcgcga catcggtggg ttcgtggcgg aggcgcaggg
     7501 ccggatcgag cggaacatcc agctgccgcc cggctactgg ctcggctggg gcggccaatt
     7561 cgagaacctg ctcgccgcgc gacgccgact cgaaatcgtc gtgccggtgt cgctggtgct
     7621 cattttcctg ctcctcttca tgacgttcgg ctcgatgaag cacgcggcgc tcgtcttcac
     7681 cggcgttccg ctggccctga ccggcggcgt gatggcgctg gcactgcgcg gcatcccgtt
     7741 ctccatcccg gcgggcgtgg gattcatcgc gctgtccggg gtagccgtgt tgaacgggct
     7801 cgtcatgatg acgttcgtca accagctgcg cgaggaaggg ctcggaatcg acgaggcgct
     7861 ccggaagggt gccgagacgc gccttcgacc gatcctcatg accgcgctcg tggcgtcact
     7921 ggggttcgtc ccgatggcgc tcgcacacgg gacaggcgcc gaggtccagc gcccgctcgc
     7981 gaccgtggtc atcggcggga tcgtctcctc gacgatcctc acgctcatcg tcctgccggt
     8041 gctctatcgg atggcccacc ggcaacgacc cgaggcctga cggtgtgatc aacaatcgtg
     8101 gctcgcttcg gcatgcgccg gagcggccaa cgatgtttac cgaattgtta catctccatc
     8161 atggcccggt tgtaaacgcg tccgtatatt ccaatacagg tccccggctg tgggaagtga
     8221 agcggctgcg tcgacgaacc ccaacggcga ggtgagcatg ctacgtatac ggatctcatg
     8281 gctcctttcc ccggctgttc tcgtgctcat cggcacgcag gtggcagcca ggccggccga
     8341 ccccggactt gaaatccggc ccgagcaggc aaatctcgct tccgatggtt tctcggctcc
     8401 gctctcgggc ctttcctccc acatctatac cgacaccgtg agctacggcg gcacctactg
     8461 ggcccctgac tcgctccgtt gggaggccat tcgcgacagc cactggtcgt tcgattcggg
     8521 tgtcggatcc tccatcaatg gcgggtccaa tccgaacaag cccgtcggct accaccagag
     8581 catggaaggg tggttcggtc tcgaccagac gttgagcccg cttccgtact tccggcgctc
     8641 gagcacctgc gccatcgccg gctcgttttc cttctgggcg ggggtgacgc tggccgaggc
     8701 caacgcgctc tgctacgccg gcggccaggg gtacggcaac aactggaacc tgatcatcga
     8761 gaagaccttc gcgtatccgg gcgtcggcac ggtgagcttc agtttccagt acgcggtgga
     8821 gtccgaggcg ggcttcgact acgcatacgc cctgatcgac acgagcggtg atggatcgac
     8881 ggacgacatc gtcctctgga cgaaagacgg tgttgcctcg ggcacagcca atgtggcact
     8941 gaacgcgggc caggacatgc gctcgaccgc cggcaattac acgatccggt tcgtggctgc
     9001 ctcggacggc tcctattcgg atgaggacgg cctcaatccc tcgacgtgcg gtcacctggc
     9061 catcgacaac gtcacggtcg gcgccgacgt aagcaacttc gagacggggg ccaacggttg
     9121 gacccagacg atttccagca ccggcgtcgg cgatttcagc cacatcgccg acctggccga
     9181 tctcgaaccg cccgcagtct tctgtccctg cgcggtacgc gactccgtgc tcgttttctt
     9241 cgacaccaac gaccagcacc cgctcgacca ggacaacctc gccgtctctc cctggatcga
     9301 cctcctcgcc aacggcgacg ccggccggtc cggcaagctc tacgcccggg acgtcgtggc
     9361 ggtcatgccg ctggaaaact atgtcttcct ccagcacctg gtccggtact acccggctgt
     9421 ctgttccgcc accggcctcg tataccgcac cccgttcctg aacctggtct cggtggaata
     9481 cttcgggccg acgccgatct gcaattcggt gctggcgccc tcccttcgcg acgcctcggc
     9541 cacgattcca cccgccgcgg aacaggtcca gctcgccgtc ggcatcatca acctgtgccg
     9601 tacgtctccg ttcggtccgc cgtgcaccgg gttcaacaac cagactcctt acctcgacaa
     9661 tctcagcctg ggcgtctacg ggagcaccac gacgcccgtg gtcaatacca cgacgttcga
     9721 tcgcttccag gacaatttcg ctaccgatgg cacgctcaat cccgcgtcga ccggccgttt
     9781 cgaccagaac cggatcaagg ggggctcgac gccgtcaagc ggctcgatcc tcggcgacac
     9841 gctgaacgcg cgtggcaatg gcggaaacac cgaggttcgg ctcgttttcc gtgttcggtc
     9901 cggccccttc atcaacgccg gtattctcgc gtcgtacgtg gcgcgctgga cgcctgagcc
     9961 cacgctgaac acccgctacg gcggcacctg gtactcggcg cgcatggaca ccgcggaaca
    10021 ggccggcacc ccggctgtct ccccgtttgc ctggatggga acgtttcatg aagccgaccc
    10081 cggtttctcc ggcacggaca ggaccgttga ccccaatgac ccaaaccgcc tggcgaacga
    10141 catcatcccg gatcacctgt tcacgcccgg ttcccgcatc gactatttcg tggcgtcccg
    10201 ctatcgcccg cccgatccgc gcaaccccgg cggtacggcc tgggctaccg acccggacac
    10261 cctgaatgcc accttccgcg aggcggagat actcccttcg tcgatggcgg cggactcctc
    10321 atggaactgc gtgctgtacg tcgatcatca cgacgaccga agcggcttcg accaggccct
    10381 cgaggagacc ggacttcggc tctcgcttgg tggtggtggc gcgaacgcgg agggaacccg
    10441 gcacgaccgt ttcgacgtgc agacgccgtc ttcggcccag atgtcgttcg gacgcccctt
    10501 gttctcccgc tacggcgtca gcctgctcca gcttttcggc ttcaagacgg tggtctggca
    10561 cagcgccacg ctcaccagcg gccagctcac gaatgaagac gcctacattc tcatgccgtg
    10621 gctgcagctc cgtgccatcg gcaacaacaa cttctgggga tcgggcgagg gtctcatgca
    10681 gtccatgcac aatacgggga gcggtgcccg cttcatgttg aacaacgtac tgggtgtcgt
    10741 gcagaactgt accggcatcc gcctggcgaa ctgcggcggc acgggcatcg acaccaccta
    10801 ctgcatcccg acctcggccg tggcaggatc gcacttcacc agcacgatcc tgccgcgcgg
    10861 aaggggcaat ggctgccccg acctgctctc cttcgaccgg ctcgacgtga acccgagtgt
    10921 cgcgtcggcc aaggggcagc tgaactatcg ccgcctcgtt cccggccagg gcggggcgtt
    10981 gaccgacatc ggcttcgcgt cggtcaccaa cctcaacacg atcgacgtga gctaccgcac
    11041 cgccctggac ggcatttcgg tcggccgcat gcgctcgaac gccggctact tcggcgtggg
    11101 gtgcgatgac gtcacggcct cgtacgagcg caccgacgac gtgctcgact ggttgggcgg
    11161 acccctgaac tgccgggttc ctcaggccct ggtggatgtg ccggaactcc ttgaccttcc
    11221 cgcgcccgcc ttccgtcacg ccctcggcaa cgcgtatccc aacccgatga atcccaccac
    11281 ccgaatccag ttcaccaacg gcacggccaa cggccgcgtg aaggtcgaga tcttcgacgt
    11341 caccggccgc ctggtccgct ccctggtgga tgcaaagatg ccggctggag ttcacgaggt
    11401 gacgtgggat ggtggcaatg aggccggctc gtccgtcccg agcgggatgt acttctaccg
    11461 catgtcggcc gaggacttcg tggcggcgaa aaagctgctc gtctcgaagt aggcacgctc
    11521 acacgtttcg tggctatcgg ccgttgctgc ccgccagagg gaagcaacgg ccgatgtcat
    11581 tcgagcggcc agatgtttac agaataatta catctccccg tgggccgagt tgtaaacgca
    11641 ttcgtatact tcaagttggg ccgtgcggtc cgcttggcaa catgttcagg aactaacccc
    11701 atcctggcga ggcgcatcat gcgagacggt aaggcactga ttctttccct ggcgctcctc
    11761 ctggcatcga gcggaccggc tcttgccagg cccatcgatc ggttgtcgga ataccggccc
    11821 gagctgtcgg atccatccat cgaggcagcc acctcgcaaa gccccggcct gtcatctcac
    11881 atcttcgggg atacggtgag ctatggtggc acctattggg cacccgactc actccggtgg
    11941 gaagccatcc gcgacagcca ctggtcattc gatacgggcg tcggctcctc gatcaatacc
    12001 ggcgccaacc cgaacaagcc ggtcggctac caccagcaga tggaagggtg gttcggcctc
    12061 gaccagacgc tgagcccgct tccgtatttc cgccgctcga gcacctgcgc catttccggc
    12121 tccttctcgt actgggccgg cgtcaccgtt cccgaagcga ccgccctctg cttcgccgcg
    12181 ggccagggat acggcaacag ctggacgttg accatcgcga agagcttcgc ctatgccggt
    12241 gcgggaaaca ttgccttcag cttccagtat gcggtcgagg ccgagcccgg cttcgattac
    12301 gcgtacgcga tgatcgacac caccggcaac ggatcgcagg acgacctgat cctctggacc
    12361 caggacggca ctgcggccgg cacggccagt atcacgttga acccgggcca ggacatgcgt
    12421 tcaacggccg gcaactacac ggtcaagttc gtcgcggcgt cggacggagg ttacgcggac
    12481 gaggacggcg cctacgccac gacctgcggc cttctcgcgg tcgacaacgt gacagtcggg
    12541 gccgacgtca gcgacttcga gacgggggac aacgggtggg cccagatgat tccgacgacc
    12601 ggtatcggcg acttcaccaa cctggcctac acgcccgacg tcgaagcgcc gccgacattc
    12661 tgtccgtgcg gcgtgcagga ctcgatcctg gtcttcttcg atccgaacga cggccacccg
    12721 atcgaccagg acaacatcgc ggtctctccc tggatcgacc tcctggccgg cggtgatgtc
    12781 ggtaaacccg gcaagctgct cctccacgat gtcgtggcca tcatgcccct caccaattac
    12841 atcttcacgc agtaccgggt ccgttactat ccggaagtct gcgcggccac ggggctgctt
    12901 taccggacgc cgtggaagga ccagaacgtc gtcttctact ggggtgagca gcccatctgc
    12961 aacccggcca atgccccacg cctccgcgac tactcggcgg tcttctcgcc gggagcgcag
    13021 caggctcagc ttgccttcgg catgatcagc ctctgccgca cctcgccgtt cgggcccctc
    13081 tgcaccggag tcacgaacca gaccccctac tacgacaaca tcagccttgg cgtgtacggg
    13141 aatgccaccg tacccgtggt caacaccaca acgttcgatc gcttccagga caatttcgcc
    13201 gccgacggaa cgctgcaccc ggccgccccg ggccgtttcg accagaaccg catcaagggc
    13261 gcctccacgc catcgaccgg gacgatcctg ggcgacacgc tgaacgcccg cggcaacggt
    13321 ggcaacaccg aagtgcgcct tgtcttccgc gtccgcaccg gtccgttcgt gaacggagcg
    13381 acgctcgcgt cctacgtggc gcgctggacg tcggagccca cactgggcgc acgctacggc
    13441 ggcaactggt actcggcccg catggacacc gcggagcagg gaggagtgcc ggcgacctct
    13501 ccattcacct ggatgagcac gttccatgag gccgaccccg gcttcatcgg caccgatcgg
    13561 gcggccgacc cgaacgaccc gaaccgcctg gccaacgaca tcatcccgga tcacctcttc
    13621 acgccgggat cgcggctcga ctatttcgtg gcggcgcgct accgaccgcc cgacccgcgg
    13681 aatccgggcg gcacggactg gagcaccgac cccgacacgg ccgggagccg gttccgcgaa
    13741 gccgaaatcc tgccatcgtc cttcgcggcg gatgccacct ggaattgcct gctgtacgtg
    13801 gaccatcatg acgatcgcga ccaggaccag cagtatctcg aggaggccgg tctccagcag
    13861 tcactcggcg ccgggagcac gaatgcggag aacacccggt acgaccgttt cgataaccag
    13921 aacccgtcgg cagcgcagtt gtccttcggc cggccgaacg gcacgaatta cggctgcagc
    13981 cagattcagg tcaatttcta caagtcgatc acgtggcaag ccgcgtcgat gacatccggt
    14041 gccgtaacgg cccaggacgt cgcgatcctc agcccgtggc tgctcctccg tgcctccgcg
    14101 aacaacgcct tctggggttc gggcgacggc attgtgcagt cgatgcacgc caccggcgga
    14161 agcacacgcg ccttcatgaa caacacgctg ggcgtcctgc agaactgcac gggcatccgg
    14221 ctggcggcgt gcggcggcgc cggtatcgac accacgtact gcatgccgct gtcgccggtt
    14281 gccgacgcct ggttcgcgag cagcgtgatt ccccatgggc gcggcaacgg ctgcccggaa
    14341 ctgcgcgcct tcgatcgcct cgatcgaaac gcgagcgcgg cctcgtccaa ggggcagctc
    14401 aactacgtcc ggctcgtccc gggcacgggg ggcgttccga ccgacatcgg ctttgcatcg
    14461 gtcgccaacc tgacgacctc cagttcgatc taccgttccg tgctcgacgg cctgggtgtc
    14521 ggaagcctgc ggaccaattc cggtgggttc ggcaccggtt gcgatgacgt gtcggcctcc
    14581 ctggcccgga ccgacgacat cctcgactgg ttcgaagccc cgctgcgctg tcgttatccg
    14641 gggctggtag gcacacccga tccaccggcc accccgtacg cgcccggctt ccgcctctcc
    14701 ctcgggccgg cctggcccaa tccgatgacg accaccacgc ggctccagtt caccaatgcg
    14761 gtgccgcagg ccgatgtccg gctcaccatc tttgacgtga cgggccgcgt ggtgcgcacg
    14821 ctccacaagg gtgaagcggc cgtgggtacg catgaactcg cctgggaccg caccaatgac
    14881 gaggggcgtc ccgtcgcagg ggggatctac ttctaccgcc tggcg
//
LOCUS       DASVOD010000400        10774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_600081, whole genome shotgun sequence.
ACCESSION   DASVOD010000400 DASVOD010000000
VERSION     DASVOD010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10774
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600081"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..425)
                     /locus_tag="VF720_09165"
     CDS             complement(24..425)
                     /locus_tag="VF720_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879567.1"
                     /translation="MPLLVGIVLAVAVGLLGRLAGFDKDRAFYPTVTIVVAHYYALFA
                     LMGAPTDILLVECLIGGVFLVAAIAGFRTSLWIVAMALAAHGVMDLVHGRFVVNAGVP
                     SFWPPFCMTFDVAAAVILAGLLATGRTRAAR"
     gene            complement(445..2817)
                     /locus_tag="VF720_09170"
     CDS             complement(445..2817)
                     /locus_tag="VF720_09170"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016878486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX7879568.1"
                     /translation="MSSRDQVSRTVLPIPDRPHVGLTTFDAKDPDTKYPPIRELRPPE
                     GAPNVLVILLDDVGFGASSAFGGPCHTPVFETLAGNGLKYNRFHTTALCSPTRQAMLT
                     GRNHHSVGMGNVCELATSAPGNSSFLPNTVSPIARTLKLNGYSTAQFGKCHEVPVWQT
                     SPMGPFDSWPTGGGGFEYFYGFIGGDTHQYYPALYEGTTPIEPEKSPEEGYHFTEDMT
                     TRSIKWIRQQKSLMPDKPFFIYYAPGATHAPHHVPAEWADRYKGQFDKGWDTLREETM
                     ARQKQLGVIPADCELTPRHSQIPAWDEMAEALKPVLTRQMELYAGFLEHTDHQVGRLI
                     ETLKELDILEDTLIYVIIGDNGASAEGSLQGTFNEMLILGGCGHLETPEFLTANLDKF
                     GGPESFNHYAVGWAHAMNTPYQWTKQVASHFGGTRNGTIVHWPKGIKSKGEIRNQFHH
                     VIDIAPTILEAAGLPEPVFVNGVQQKPIEGVSMAYSFDDANAAGRHETQYFEMIGNRG
                     IYHKGWTAVTRHRTPWETGAVVLPAFDDDVWELYDTTTDWSQAKDVSKEHPEILHELQ
                     RLWLIEAVKYNVVPLDDRFAERANADLAGRPQLVRGNRQLLFGGMGRLSESSIVNMKN
                     KSHAVTAEIEVPEKGAQGVIVAQGGMIGGWSLYAKDGKPKYCYNYFGLNRYVVEGSTT
                     IPSGKHQLRMEFTYDGGGLGKGGLATLYLDGKKVGEGRIDQTQAFVFSADETCDIGDE
                     FGSPVTTDYPAHKKFSGEVNWVQLDVDKDAEDPDHYITPEERLRVIMARQ"
     gene            3076..3444
                     /locus_tag="VF720_09175"
     CDS             3076..3444
                     /locus_tag="VF720_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7879569.1"
                     /translation="MSRPVHFEIPADNVERAQAFYQNVFGWQFQKWDGPMEYWMIVTG
                     PDGTPGINGGLGRRRMPDEKPVNTIRVKSVDAAVAGITKSGGSVTVPRMAIPGVGWLA
                     YAVDTEGNPFGVMEEDVNAK"
     gene            3721..5265
                     /locus_tag="VF720_09180"
     CDS             3721..5265
                     /locus_tag="VF720_09180"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013374301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7879570.1"
                     /translation="MADGVTTDRLYGPDGGKMFGVLVARDGTGGMRVIKAFSGQWGPA
                     WELPGWAPPIFDAAARNAIEPAVEARIKSLGQAMEELAEDPAFRAERGSRQEVRHRYR
                     VARADLKAEQVARRANRHARRVRLADDDRAGRRLLDNESRREDIEHRHRHVVLREAEL
                     REIRPNRRLERRFGAMERLRRRLSRLAMSSILDTYVLTSFRGTTTRLRSMFGDAGPPW
                     GAGDCAAPKLLAAAMRENLVPVALAEFWWGGPPPGGGRVEGHFYPACAPKCGPILPFM
                     LDGLDVAPRRTWKPSEPESDHLDIRYDDGRIVVLSKPAGLLSVPARDESISDSVFARL
                     KRRYPAATGPLLVHRLDLDTSGLLVVALDEGTHRELQAQFESRNVQKRYVAWLGGELE
                     ADRGTISLPMRVDLEQRPRQVVDFVHGREAITDWKVIERVGGRTKVALFPRTGRTHQL
                     RVHAAHREGLNAPIVGDRLYGTAGERLLLHAEAITFRRPDGRPMTVSDPAPFGNVRAD
                     RGSDSA"
     gene            complement(5349..6293)
                     /locus_tag="VF720_09185"
     CDS             complement(5349..6293)
                     /locus_tag="VF720_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879571.1"
                     /translation="MAAGISLLVKSVPARAASPETPDECLRRLVSQALASGSITDDSL
                     MVDGFSRSGLDPLSSEQRAGVREHLDLFAPVLAQAQAPSHSPGWDIQILFLEQGTPEQ
                     RAMMTSQLRRWFTEDVRQPLRDRSVRYENAQAIREVVLGYRVTVAEMLAEYGDRSATR
                     VIREWSMGESMETEDRDRVIQALARLEDPCHRRWLIPAGVDRVEPCDGSEPLTVRACH
                     RRGESTDCGDPIPAAAARALDHVMSLVPVRRAGPRSEGSRWLSLTGGGRECRIEPAAN
                     GKSVIIAESDMHEFGFAWEVESAELAAWVIGWMKTAPR"
     gene            complement(6510..7493)
                     /locus_tag="VF720_09190"
     CDS             complement(6510..7493)
                     /locus_tag="VF720_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879572.1"
                     /translation="MTRLFVARAAMLTILIGALLTIDAAAQPAANPNAVGVPPEPIPV
                     ESPQAALHRMVGAALAAGTVSDEAALAASLGQLGLEPLSAAERNGIRESLDLFTSVLV
                     GAQAPGYEPNWDIQLLLLERGSTEQRARMSGQLRRWFTESVRGADTNENCARVAEMLA
                     EYGDQASTPAIRRWMDQGSVDSAQRERATRAVARIGNPCQGRWLTAEGINEVQLCPGA
                     GLINVSACWYIDGSRDCGTSIPIESARYLARTIRLQTVRRVPLSTVAHLALAISAKSD
                     IDVRIFPTDDARAIYIVERDMQASGLAWRADSPELAKWAIGWLKTAPPHTP"
     gene            complement(7490..8665)
                     /locus_tag="VF720_09195"
     CDS             complement(7490..8665)
                     /locus_tag="VF720_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879573.1"
                     /translation="MAETDFRRVLDIVWDDAERTGFAGWDPYDGLNGKLFRSTPLFRI
                     PVARQAWIQLVKRMPVNLRPLTFQKPEVLAKGVSLFAMGSWRLAGAAEARGDSGRAGT
                     WRRRGGALLDRLVGLRSPGWDEICWGYPYDWQSRAFFQPRDCPNLICSVFGAMAFERR
                     NDGGAAHAVPDEVSRFVMRALSRTEDEAGYITYTPTTNTRVHNVNMLGAALLARSAKR
                     ANDTKLAAFAAESMRFSVDRLLPNGSWPYGESPNQAWVDNFHTGYNLVALEDYRLTTD
                     DSSMDAALEKAYHYWDRTFFRADGAPSYYDNSHYPIDIHCCAQAILTWLDLERLDPDA
                     FAKARKTAEWTMHHMWNGRHFTFQRGSWWRNDVKQIRWGQAWMFWALSELVAAEARS"
     gene            complement(8669..>10774)
                     /locus_tag="VF720_09200"
     CDS             complement(8669..>10774)
                     /locus_tag="VF720_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HEX7879574.1"
                     /translation="VPTPRAQAGSILVRNAWSLVSAGTERSTVSTGQKSLLGKARARP
                     DLVKKVIDSARKEGVVAAIQKVQARLDTWKTLGYSTAGVVIEVGEGVEGFAVGDRVAC
                     GGQDKASHADYVLVPANLCARVPDGVDLKHACFTTVGVIALQGVRQAEVKLGERIAVI
                     GLGLVGQLTVQILKAAGCQVLGIDVNAGACELALKSGADRVAVRGTDDVEAIAAGMTN
                     GFGVDAILITAAASTNDPVELAAKIARDRATVVMVGVTGMDLPRDLYFSKDLQFRLSR
                     SYGPGRYDALYEEAGVDYPIGYVRWTEGRNFEAFLDLIAAKRLDLDLLTTHSFRIDDA
                     LKAYDVITGKTNERFIGVLIEYPDAASRMQRSVAVGPIGNGSTSGKVVLGVIGAGNYA
                     QAMLLPHLKGRSDVELAAVATSSGVTARKVAERSGFAIAATDPDEVLKNDRINAVIIA
                     TRHHLHARYILAALERGKAVFVEKPLCMNREELDQIAAAMSRKDARVQVGFNRRFAPT
                     AVAAREFMSQAATQTITYRVNAGPLPANHWLRDPEQGGGRLIGEGCHFIDFLIFMAGA
                     LPERVHARGLGGGAQDAVDSWVLDMGFANGSIGQILYTATGDPAFPKERMETFGAGRV
                     AVIDDYKTGSLSSGGNRKATGSSAQDKGQGPQVAAFLDMVAKGGPPPIPHDQLLAGAS
                     AIFGALESMREGRDIEVRR"
BASE COUNT         1765 a         3554 c         3590 g         1865 t
ORIGIN      
        1 gcccaccctc ttcaggaatc gcgtcatcgc gcggcgcgcg tgcgcccggt tgccagcaat
       61 cccgccagga tcaccgcggc ggcgacatcg aacgtcatgc agaacggcgg ccagaagctc
      121 ggtacgcctg cattgacgac gaatcggcca tgcacgaggt ccatgacccc gtgggccgcg
      181 agtgccattg cgacgatcca caaggatgtg cggaatcccg cgatcgctgc caccaggaac
      241 actcccccga tcaggcactc gaccaggaga atgtcggtgg gcgcgcccat gagcgcgaag
      301 agtgcgtagt agtgcgcaac gacgatggtg acggttgggt agaaggcccg atccttgtcg
      361 aaccccgcca gcctccccaa aagacctacg gccactgcca gcacgattcc aacgagtagc
      421 ggcactgtag tccccctccc ctgtttactg ccgcgccatg atcacccgca accgctcctc
      481 cggcgtgatg tagtgatccg gatcctcggc gtccttgtcc acgtcgagct gcacccagtt
      541 cacctccccg ctgaacttct tgtgggccgg atagtcggtg gtgaccgggg atccgaactc
      601 gtcgccgatg tcgcaggtct cgtcggcgga gaagacgaag gcctgggtct ggtcgatccg
      661 gccctccccg accttcttgc catccaggta gagggtggcc aatcccccct tgccgagacc
      721 tccgccgtcg taggtgaact ccatccggag ctggtgcttt ccggatggga tcgtcgtcga
      781 tccctccacc acgtagcggt tcaggccgaa atagttgtag cagtatttcg gcttgccgtc
      841 cttcgcataa aggctccagc cgccgatcat cccgccctgg gccacgatga cgccctgggc
      901 gcccttctcc ggcacctcga tctcggcggt cacggcgtgg gacttgttct tcatgttcac
      961 gatcgagctc tccgacaggc gccccatgcc accgaatagc agctggcggt ttccccgcac
     1021 gagctgtgga cgtccggcaa gatccgcatt ggcccgttcc gcgaaccggt cgtcgagcgg
     1081 aacgacgttg tacttgacgg cttcgatcag ccaaagccgc tggagttcat gcagtatctc
     1141 cgggtgctcc ttcgacacgt ccttcgcctg gctccagtcc gtcgtcgtgt cgtagagctc
     1201 ccagacgtcg tcgtcgaagg ccggcagcac cacggcgccg gtttcccacg gagtccggtg
     1261 gcgcgtcacg gccgtccacc ctttgtggta gatgcctcgg ttgccgatca tctcgaagta
     1321 ctgcgtttcg tgccggccgg ccgcgttcgc atcgtcgaac gaataggcca tgctcacccc
     1381 ttcgatcggc ttctgctgaa ccccgttcac gaaaaccggc tcagggaggc cggctgcctc
     1441 caggatggtc ggggcgatgt cgatcacgtg atggaactgg ttgcgaatct cacctttcga
     1501 cttgattccc ttcggccagt gcacgatcgt cccgttgcgc gtacctccga agtgcgaggc
     1561 gacctgcttg gtccactggt acggcgtgtt catcgcatgc gcccagccaa ccgcgtagtg
     1621 gttgaacgat tcggggccgc cgaacttgtc caggttggcc gtgaggaact cgggcgtctc
     1681 gaggtggccg cagccgccga ggatgagcat ctcgttgaac gtcccctgca gggacccctc
     1741 ggcggaggcg ccgttgtcgc cgatgatgac gtagatgagg gtgtcttcga gaatgtcgag
     1801 ttctttgagc gtctcgatga gccgcccgac ctggtgatcg gtatgctcga ggaagccggc
     1861 gtacagctcc atctggcggg tgagcaccgg cttcagcgct tcggccatct cgtcccacgc
     1921 cgggatctgc gagtgcctag gcgtgagttc gcaatccgcg gggatgacac cgagctgctt
     1981 ctggcgggcc attgtctcct cgcggagcgt gtcccacccc ttgtcgaact ggcccttgta
     2041 cctgtcggcc cactcggccg gcacatggtg cggggcatgc gtggcccccg gcgcgtagta
     2101 gatgaagaac ggtttgtccg gcatgagcga cttctgctgg cggatccact tgatcgaccg
     2161 cgtcgtcatg tcctcggtga agtgatatcc ctcttccgga ctcttttccg gttcgatcgg
     2221 tgtcgtccct tcatagaggg ccgggtagta ttggtgcgtg tcgccgccga tgaagccgta
     2281 gaagtactcg aatcctccgc ccccggtcgg ccacgaatcg aacggcccca tcgggctggt
     2341 ctgccacacc ggcacctcgt ggcacttgcc gaactgcgcc gtcgagtagc cgttgagctt
     2401 cagggtccgg gcgatcggcg agaccgtgtt cggcaggaac gacgaatttc ccggcgccga
     2461 ggtggccagc tcgcagacat tgcccatccc gaccgagtgg tgattgcgcc ccgtcagcat
     2521 cgcctggcgt gtcggcgaac agagcgcggt cgtgtggaag cggttgtact tgagtccgtt
     2581 tccggcgagc gtttcgaaca ccggcgtgtg gcaggggcca ccaaaggcgc tgctcgcgcc
     2641 gaaaccgacg tcatcgagca gaatcaccag gacgttcggc gcgccttcgg gcggccggag
     2701 ctcgcggatg gggggatatt tcgtgtccgg atccttggca tcgaacgtgg tcaggccgac
     2761 gtgcggccga tcgggaattg gaagaacggt gcgtgaaacc tggtcccttg agctcatcca
     2821 tctctcctga tgacccgatt gcccgggtgc ggcgcatcga tcccgtccgg gatcggggcg
     2881 gaattatgcc agcggagagc gttgacagaa aggcggggag gggatctcca ggctcgtgga
     2941 cttgattcct gatcatgaca acgagccggt ggcgggccgg accctcgaga aatcgtctaa
     3001 aggattccgt cgatccgttg gatggaggtt cgggcgcaac gcccgagcct gaaaccacaa
     3061 ccaacgaggt gacccatgtc acgacccgtt catttcgaga tcccggccga caacgtcgag
     3121 cgggcccagg cgttctacca gaacgtcttc ggctggcagt tccagaagtg ggacggcccg
     3181 atggagtact ggatgatcgt caccgggccg gatggcacgc ccggcatcaa cggcgggctc
     3241 gggcggcggc ggatgccgga cgagaagccc gtcaacacga tccgggtgaa gtcggtcgac
     3301 gcggccgtgg cgggcatcac gaagtcgggt gggtcggtga cggtgcccag gatggcgatt
     3361 ccgggggtcg gctggctggc ctacgcggtc gataccgagg ggaatccgtt cggggtcatg
     3421 gaagaggatg tgaacgcgaa gtagcggagt ccagacttcc cgaccgcgtt ggccgcttcc
     3481 attggctcga gcccgcgccg atgttcggag ggggcggcgc ggtcgggacg gtatatcacc
     3541 ttgaggccgc gtcggcgggc cttcatcatg tgcgcctcta tggagtcacc ccttttcgat
     3601 tctggacgtg ccgtgccggc ggagtcacga cccttccctt cgccgttcga tgacctccct
     3661 ccacacccgc ttgcccagga agctgccctc gatctgcagg cggggttgcg gaccggaagc
     3721 gtggccgacg gagtcacgac cgaccggctg tacggcccgg acggggggaa gatgtttggc
     3781 gtgctggtgg cgcgcgacgg gaccggcggg atgcgggtca tcaaggcgtt ctcgggccaa
     3841 tggggtcccg cgtgggaatt acctggctgg gcgcctccga tctttgatgc ggcggcccgg
     3901 aatgcgatcg agccggcggt cgaagccagg atcaagtcgc tgggccaggc catggaggag
     3961 ttggccgagg acccggcgtt tcgcgccgag cgcggttcga ggcaggaggt gcgccaccga
     4021 tatcgcgtgg cccgcgcgga cctgaaggcc gaacaggttg cgcgacgcgc caatcgccat
     4081 gcccggcggg tgcggctggc cgacgacgat cgggctggcc gccggctcct cgataacgag
     4141 agtcgtcggg aagacatcga gcatcgacat cgtcatgtgg tgcttcgcga ggcggagctt
     4201 cgcgaaatca ggccgaatcg gcggctcgag cgacggttcg gggccatgga gaggctgcgg
     4261 cgccggttgt cgcgcctggc catgtcgagc attctcgata cctatgtgct gaccagtttc
     4321 cgggggacaa cgacccggtt gcggtccatg ttcggcgacg cggggccacc gtggggcgcc
     4381 ggtgactgcg cggcgccgaa actgcttgcc gcggcgatgc gcgagaatct cgtaccggtg
     4441 gcgctggctg agttctggtg gggaggcccg ccacccggcg gtggacgggt cgaggggcat
     4501 ttctaccccg cctgcgcgcc gaagtgcggg ccgatccttc ccttcatgct ggatggcctc
     4561 gacgtggcgc ctcgtcggac ctggaagccc tcggagccgg agtcggacca tctcgacatc
     4621 cgctacgacg acgggcggat cgtggtgctg tcgaagccgg cggggctcct gtcggttccg
     4681 gcgcgcgatg aatctatttc cgactcagtc ttcgcgcgat tgaagcgccg gtatccggcg
     4741 gccacgggcc ccctgctcgt ccatcggctc gatctcgaca cgtcgggcct cctggtggtg
     4801 gcgctcgacg aagggacgca ccgtgagctg caggcccagt ttgagtctcg caatgtgcag
     4861 aagcgctatg tggcctggct cgggggagag cttgaggccg accgcgggac gatttcactg
     4921 ccgatgcggg tggacctcga gcagcggccg cgacaggtcg tggacttcgt ccacggcagg
     4981 gaggccatca ccgactggaa agtgatcgag cgggtcgggg ggcggacgaa ggtggcgctc
     5041 ttcccgcgga cgggccggac acaccagctg cgggtgcatg cggcgcaccg ggagggactc
     5101 aacgccccga tcgtgggtga ccggctgtat ggaaccgccg gcgagcggct gctgctgcac
     5161 gcggaggcga ttacgtttcg tcggccggac gggcggccga tgaccgtcag cgacccggct
     5221 ccattcggga atgtgcgggc tgaccgaggc agcgactcgg cctgaacata gttggtccgg
     5281 cgaccaggtt tacgcgccga ctctgcctga acatagttgc cgcccgtgcg gcaagagtct
     5341 cgtaaccctc agcgcggggc tgtcttcatc catccgatga cccacgcggc gagctcggcg
     5401 ctttccactt cccaggcgaa gccgaactca tgcatgtcgc tttcggcgat gatgaccgac
     5461 ttgccgttcg cggccggttc gatgcggcac tcgcggccgc ctccggtcag gctgagccac
     5521 cggcttccct cggaacgggg accggcccgg cggaccggca ccaggctcat gacatgatcg
     5581 agcgccctgg ccgcggccgc cgggatcgga tcgccgcagt ccgtggactc gcccctgcga
     5641 tggcaggcgc gcacggtgag cggctcggag ccgtcgcaag gctcgaccct gtccacgccg
     5701 gcggggatca gccagcgtcg gtgacaggga tcctctagtc gtgcgagggc ctggatcacc
     5761 cggtcccgat cctcggtctc catcgactcc cccatcgacc actcgcgaat gacgcgcgtg
     5821 gcggaccgat cgccgtattc cgcgagcatc tcggccaccg tgacgcgata gccgagcacc
     5881 acctcgcgaa tggcctgggc attttcatac cggaccgatc gatcccggag aggttggcgc
     5941 acgtcttcgg tgaaccagcg acggagctgg gacgtcatca tggcgcgctg ttcgggtgtg
     6001 ccctgttcga gaaacaggat ctggatgtcc cagcctggcg agtgcgatgg agcctgggcc
     6061 tgcgcgagca ccggggcgaa gagatcgaga tgctcgcgga ccccggcccg ctgctcggag
     6121 gacaagggat caaggccgga acggctgaag ccgtccacca tcaacgagtc gtcggtgatg
     6181 gagcctgagg cgagagcctg cgagacaagg cggcgaaggc actcatccgg cgtctcggga
     6241 gacgccgccc gggccgggac gctcttgacg agcaggctga tgccggcggc catcgcgaca
     6301 caagccaaca gacgtcggcc gaatcgggtg gggtgcatgc agggcactcc atcggatcga
     6361 tccggtgcgc cgcggccgcg tcgtccgggt tcgacctcgt ccgagcttcg gacagtaaga
     6421 cgaaaagttt acatcatctt ggatgccgtg gtggcagata gtcgtaccac cggactcgcg
     6481 gagccgggat gcccgtctgc gatggccgat tacggtgtat ggggaggtgc cgtcttcaac
     6541 cacccgatcg cccacttggc aagctccggg ctgtcggccc gccacgccag tccggaggcc
     6601 tgcatgtcgc gctcgacaat gtagatcgcc cgggcgtcgt cggtcgggaa gatgcggacg
     6661 tcgatgtccg acttggcgct gatcgccagg gccaggtggg ccacggtgga gagaggaacg
     6721 cggcgcaccg tctggaggcg gatcgtgcgc gccagatagc gggccgactc gatgggaatg
     6781 ctcgttccgc agtcgcggct cccgtcgatg taccagcagg ccgagacgtt gatcaatccg
     6841 gcgcccgggc agagctgcac ctcgttgatc ccttcggccg tcaaccatct cccctggcag
     6901 ggattgccga tgcgggccac cgcccgtgtc gcccgctcgc gttgggcgga atcgaccgac
     6961 ccctggtcca tccagcggcg gatcgccggc gtcgaggcct ggtcgccgta ctcggcgagc
     7021 atctcggcaa cccgggcgca attctcattg gtatccgctc cacggacaga ttcggtaaac
     7081 caccggcgaa gctgccccga catccgggca cgctgctccg tgcttccccg ctcgagcagg
     7141 aggagctgga tgtcccagtt cggttcgtag cccggggcct gagccccgac gagaaccgac
     7201 gtgaacaggt cgaggctctc ccggatgccg tttcgttccg cggcgctcaa aggttcgagc
     7261 ccgagctgac ccagggatgc cgcgagtgcg gcctcatcgg agacggtccc ggccgccagg
     7321 gccgcgccga ccatgcgatg aagcgccgcc tggggggatt cgacgggtat cggttcaggg
     7381 ggcaccccga ccgcgttcgg gttggcagcc ggctgggcgg ccgcgtcgat cgtcaggaga
     7441 gcaccgatga ggatcgtcag catggcggcc cgggcgacga acagacgagt cacgaccgcg
     7501 cctccgccgc caccagttcc gacaacgccc agaacatcca cgcctgtccc caccggatct
     7561 gcttcacgtc gttccgccac cacgaccccc tctggaaagt gaagtgtcgg ccattccaca
     7621 tgtgatgcat ggtccactcg gctgtctttc gtgccttggc aaacgcgtcg ggatcgagac
     7681 gctcgaggtc gagccaggtc aggatcgcct gtgcgcagca gtggatgtcg atcgggtaat
     7741 gactgttgtc gtaataggac ggggcaccgt cggccctgaa gaatgtcctg tcccagtagt
     7801 ggtacgcctt ttcgagcgcg gcgtccatgg aggaatcatc ggtcgtcagc cggtagtcct
     7861 ccagcgccac gaggttgtag ccggtgtgaa aattgtccac ccaggcctgg ttgggtgatt
     7921 cgccgtaggg ccaggagccg ttcgggagga gccgatcgac cgaaaagcgc atcgactcgg
     7981 cggcgaaggc ggccaacttc gtgtcattcg cccgtttcgc actgcgggcc agcagggccg
     8041 cgccgagcat gttcacgttg tgcacacgcg tgttggtcgt gggcgtatag gtgatatagc
     8101 ccgcctcgtc ctcggtgcgc gaaagcgccc gcatcacgaa gcgtgacacc tcgtcaggaa
     8161 cggcatgagc cgctcccccg tcgttgcgcc gctcgaatgc catcgcgccg aaaaccgagc
     8221 agatgaggtt cggacaatcg cgcggctgga aaaacgcccg gctctgccag tcatacgggt
     8281 agccccagca gatttcgtcc cagccggggg atcggagccc gacgagacga tcgagcaacg
     8341 ccccgccgcg ccgacgccac gtcccggcgc gcccggaatc gccccgcgcc tcggcagcac
     8401 ccgccaggcg ccacgacccc atcgcgaaga gggacacccc cttcgccaac acttccggct
     8461 tctgaaacgt gagcggccgc aggttcaccg gcatgcgctt cacgagctga atccacgcct
     8521 gccgcgcgac cggtatcctg aagagcggcg tcgagcgaaa cagtttccca ttcaggccgt
     8581 catacggatc ccagccggcg aaaccggtgc gctcggcatc gtcccagacg atgtcgagga
     8641 cgcggcggaa atccgtctcg gccattacct agcggcggac ctcgatgtcg cgcccttcgc
     8701 gcatcgattc cagcgcgccg aagatcgcgg aggccccggc caggagctgg tcgtgcggga
     8761 tcggcggcgg gccacccttg gccaccatgt cgaggaaggc ggccacctgc gggccctgtc
     8821 ccttgtcctg ggccgagctg ccggtggcct tccggttgcc gcccgacgac agcgagccgg
     8881 tcttgtaatc atcgatgacc gccacgcgcc ccgcgccgaa cgtctccatg cgctccttcg
     8941 ggaacgccgg atcgccggtg gccgtgtaga ggatctggcc gatcgagccg ttggcgaagc
     9001 ccatgtcgag cacccatgaa tcgacggcat cctgcgcccc gccaccgagc ccgcgggcat
     9061 gcacacgctc gggcagcgcg ccggccatga agatcaggaa gtcgatgaag tggcaccctt
     9121 cgccgatcaa ccggccgcct ccctgctcgg gatcgcgcag ccagtggttg gccgggagcg
     9181 gtccggcgtt cacgcggtag gtgatcgtct gggtggccgc ctggctcatg aactcccgcg
     9241 cggcaaccgc ggtcggcgca aaacgccggt tgaagccgac ctgcacacgc gcgtccttcc
     9301 gcgacatggc cgcggcaatc tgatcgagct cctcgcgatt catgcagagc ggtttctcga
     9361 cgaagaccgc cttcccccgc tccagtgccg cgaggatgta cctggcatgg aggtgatgcc
     9421 gtgtggcgat gatgaccgcg ttgatccggt cgttcttgag cacctcgtcc ggatcggtgg
     9481 ccgcgatggc gaatccgctc cgctcggcca cctttcgcgc ggtcaccccg ctggaagtgg
     9541 ccacggcggc aagctcgaca tccgaccgcc ccttgagatg gggcagcagc atcgcctgcg
     9601 cgtagttgcc ggcgccgatg acgccgagca ccaccttgcc gctcgtcgag ccgttcccga
     9661 tgggacccac ggccaccgag cgctgcatgc ggctggcggc atccggatat tcgatcagca
     9721 caccaatgaa gcgctcgttc gtcttgcccg tgatgacgtc gtacgccttg agcgcgtcgt
     9781 cgatccggaa cgaatgagtg gtcaacaggt ccaggtcgag ccgcttcgcg gcgatcaggt
     9841 caagaaacgc ctcgaagttg cgtccttccg tccagcgaac atagccgatg ggatagtcga
     9901 cacccgcctc ttcgtacagg gcgtcataac gtcccgggcc gtaggaccgg gacaaccgaa
     9961 attgaagatc cttggaaaag tagagatcgc gcggcaggtc catgccggtg acgccgacca
    10021 tgaccaccgt ggcccgatcg cgcgcgatct tcgcggcgag ttcgaccgga tcgttggtgg
    10081 aagccgccgc cgtgatcagg atggcatcca cgccgaagcc gttcgtcatg ccggccgcaa
    10141 tggcctcgac atcgtccgtg ccgcgcacgg ccacccggtc ggcgccgctc ttcaatgcca
    10201 gttcgcaggc ccccgcgttc acatcaattc cgagcacctg gcaaccggcg gccttgagga
    10261 tctgcaccgt aagctgcccc acaagcccga gcccgatcac cgcgatccgc tcgccgagct
    10321 ttacctccgc ctggcgcacg ccctggagcg cgatgacgcc gaccgtcgtg aagcaggcgt
    10381 gcttcaggtc cacaccgtcc ggcacgcgcg cgcagaggtt ggccggcacc agcacgtagt
    10441 cggcgtggct cgccttgtcc tgcccgccgc acgccacgcg gtcgccgacg gcgaaacctt
    10501 ccaccccctc gcccacttcg atgaccacgc ccgccgtcga atagccgagc gtcttccagg
    10561 tgtcgagccg cgcctgcacc ttctggatgg ccgccacgac cccttccttg cgggccgagt
    10621 cgatgacctt cttcacgagg tcggggcgcg cgcgggcctt gccgagcagg ctcttctggc
    10681 cggtcgagac cgtcgaccgt tccgttccgg ccgacacgag gctccatgcg ttgcgcacca
    10741 ggatggagcc cgcctgcgca cgcggcgtcg gcac
//
LOCUS       DASVOD010000401         3981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1020761, whole genome shotgun sequence.
ACCESSION   DASVOD010000401 DASVOD010000000
VERSION     DASVOD010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020761"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..3333)
                     /locus_tag="VF720_09205"
     CDS             complement(109..3333)
                     /locus_tag="VF720_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879575.1"
                     /translation="MRKRLGTTILLLALAAGAVEARDTGQTTTRLKPFQRAAGTETGT
                     TSSVSHLSAFGGVDTMYYGGTFWASDSARWEAVRDSCWTFDTGVGSAFGATGPNKPVG
                     YHTRMEGWYGRDATLNPIPYFRRSSTCVIAGSFSFWAGVNAAEATGLCWAAGQGYGNN
                     WDLTIAKTFNYPGSGNITLSYKYAVDAEPGYDYMYVLVDTTGNGSQDDLVLNSYTGTV
                     SGTGSVILNPGSDLRSNAGPITIKFVASSDGSYSDEDGLNPTSCGHSAIDDIGLTGAV
                     VDNSNFESGANGWVQVIPTTGIGDFSNLGSLADLPPPATFCPCAMSDSVLVFFDTNDS
                     HPLDQLNFAVSPWIDLGRGGDAGRPGKLLTADVYSEMPAANYVFEYQQIRWFPYTCPQ
                     NGQIITSPWRDQNTVFYYGETPFCTQTGAPAFTDYSGIVDAAAQEVQLSWGVINLCRT
                     APFGFACTGQTNTTPYLDNLALGVYGEANAPTVIITTFEILQDNFAADGTLNPAATGV
                     LDVNNIISAGAPEAGATLGDTLTVTGDGGNTEVRLVFHVRPGPFTNAGSLASRATRWR
                     PEPGLGSNWYSARMDSAERGGINIPTTWMATFHESDPGFQGTDTTPDAGNPNQLANEI
                     IPDNLFTPGSRIEYFIASRYLPGDPRNPGGTNWTTNPDTAGRTYFEVEILPSSMAADS
                     SWNCTLYVDHHHDREVADQNIITAGLNASLGLGGNNAEGTRFDRFDNEAPSSNQVSFG
                     RPLDTNWGASIIQVFGYKNILWFTGTLNGEQITQEDAAILNPWLLIRAIGNNRFYGSG
                     DDLATAMHLSGKAGPTQFLRNTMGALRNCNTIRNAACPTGTVVDSTFCLPVSAVAGSH
                     FPLAGTNRIRGNGCPNLRSYDLLSVNPAVTTARGNINYVKSGVDRNYASITNWNTVDV
                     DYKTVLDGFQVGRLRSNAGFFGVGCDQVAAAHNRLDAILDWFGTAATCKVPAALVDVP
                     VDGGPGVPGFRHALGNAYPNPMNPTTRIQFTNGVANGRVKLEIFDISGRLVRTLVDDR
                     MSAGVHEVTWDGMAQKGNPVPSGMYFYRMTADDFVSAKKLVVSK"
     gene            3706..>3981
                     /gene="ssb"
                     /locus_tag="VF720_09210"
     CDS             3706..>3981
                     /gene="ssb"
                     /locus_tag="VF720_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX7879576.1"
                     /translation="MASRGVNKVILVGNLGADPEIRYTTSGTAVATLRLATTESWMDK
                     SGERTERTEWHRVIVWGKSAETASQYLSKGKQLYVEGRLQTREWTDKD"
BASE COUNT          672 a         1182 c         1422 g          705 t
ORIGIN      
        1 cggcgcatcc tgacatcgac cggcaccgcc ggtcaccaca aaaaaaccgg gtgcgggagg
       61 gctttcctcc cgcacccggt gcgcctgcgc gcaacgatgt tcctgaggtt acttcgagac
      121 gaccagcttc ttcgccgata cgaagtcgtc ggccgtcatg cggtagaagt acatcccgct
      181 cggaacaggg ttgcccttct gagccatgcc gtcccaggtc acctcgtgaa cgcctgccga
      241 catccgatcg tccaccagcg tccgcacgag gcggccggag atgtcgaaga tctcgagctt
      301 gacccgaccg ttcgccacgc cgttggtgaa ctggatgcgc gtggtgggat tcatcgggtt
      361 cgggtaggcg tttccgaggg cgtgacggaa tccggggacc cctggaccac cgtcgaccgg
      421 gacgtcgacc agggccgccg gaaccttgca tgttgccgcg gtaccgaacc agtcgaggat
      481 ggcgtccagg cgattgtggg cagcggccac ctggtcacag ccgacgccga agaatcccgc
      541 gttcgaccgg agccggccga cctggaatcc atcgagcacc gtcttgtagt ccacgtcgac
      601 cgtgttccag ttggtgatgg aggcgtagtt gcgatccacc cccgacttca catagttgat
      661 gttgccacgc gccgtcgtca ccgccgggtt caccgacagg aggtcgtacg agcggaggtt
      721 cgggcagccg tttccgcgga tccggttggt tccggccagc gggaaatggg agccggccac
      781 cgccgagacg gggaggcaga aggtggagtc gaccacggtt cccgtggggc aggccgcgtt
      841 ccggatggtg ttgcagttcc gaagcgcgcc catcgtgttc cggaggaact gcgtgggacc
      901 ggccttgccc gagagatgca tggccgtggc caggtcatcg cccgaaccgt agaagcggtt
      961 gttgccgatc gcccggatga ggagccacgg gttcaggatg gccgcgtcct cctgggtgat
     1021 ctgctcgccg ttcaacgtgc cggtgaacca gaggatgttc ttgtagccga acacctggat
     1081 gatcgaggca ccccagttcg tgtcgagggg gcgtccgaac gacacctggt tcgaggaagg
     1141 cgcttcgttg tcgaagcggt cgaaccgggt tccttcggcg ttgttgccgc ccaggccgag
     1201 gctggcgttg aggccggccg tgatgatgtt ctggtcggcc acttcacgat cgtggtgatg
     1261 atcgacgtac agcgtgcagt tccagctcga gtcggccgcc atcgacgagg ggaggatttc
     1321 cacctcgaag taggtgcggc cggccgtgtc cgggttggtg gtccagttgg tgccgcccgg
     1381 gttgcgcgga tcgcccggca ggtatcgcga ggcgatgaag tactcgatcc ggcttccggg
     1441 agtgaacagg ttgtcgggga tgatctcgtt ggcgagctgg ttgggattgc cggcgtccgg
     1501 cgtggtgtcc gtcccctgga agcccgggtc gctctcgtgg aacgtggcca tccaggtggt
     1561 ggggatgttg atgccgccac gctcggccga gtccatgcgc gccgagtacc agttgctgcc
     1621 gaggcccggc tccggccgcc agcgggtggc gcggctggcg agggaaccgg cgttggtgaa
     1681 cggtccggga cgcacgtgga agacgagccg gacttcggtg ttgccgccgt cgcccgtcac
     1741 ggtcaacgtg tcacccagcg tggcgcccgc ttccggggct ccggccgaga tgatgttgtt
     1801 gacgtcgagg acgccggtgg ccgccgggtt cagcgtgccg tccgccgcaa agttgtcctg
     1861 gaggatttcg aacgtggtga tgatgacggt cggtgcgttg gcctcgccgt agacgccgag
     1921 cgccaggttg tcgagatacg gcgtggtgtt ggtctgtccc gtacaggcga acccgaacgg
     1981 ggccgtgcga cagagattga tgacgcccca ggagagctgg acctcctggg cggcggcatc
     2041 gacgatgccc gagtagtcgg tgaacgccgg ggcaccggtc tgggtgcaga acggggtttc
     2101 tccgtagtag aagacggtgt tctggtcacg ccacgggctg gtgatgatct ggccgttctg
     2161 cgggcaggtg taagggaacc agcggatctg ctggtactcg aacacgtagt tggccgccgg
     2221 catttccgaa tagacgtcgg cggtcaggag cttgcccggt cggcccgcgt cgccaccgcg
     2281 gccgaggtcg atccacggcg agacggcgaa gttcagctgg tcgagggggt gggagtcgtt
     2341 ggtgtcgaag aacaccagga ccgaatcgct catggcgcag ggacagaacg tggccggcgg
     2401 gggcaggtcg gccagcgacc ccaggttcga gaagtcgccg atgcccgtgg tcgggatgac
     2461 ctgcacccag ccattggcgc cgctttcgaa gttgctgttg tccacgaccg caccggtcag
     2521 cccgatgtcg tcgatggcgc tgtggccgca gctggtggga ttgaggccgt cctcgtcgct
     2581 gtacgagccg tccgaagagg ccacgaactt gatggtgatc ggtccggcgt tgctgcggag
     2641 gtcggagccc ggattcagga tgaccgatcc cgttcccgaa accgtgccgg tgtagctgtt
     2701 caggacgagg tcatcctggg agccgttgcc ggtggtgtcg acaagcacgt acatgtagtc
     2761 gtagccgggt tcggcgtcga cggcgtactt gtagctgagg gtgatgttgc cggagccggg
     2821 atagttgaac gtcttggcga tcgtcaggtc ccagttgttc ccgtacccct ggccggcggc
     2881 ccagcagagc ccggtggctt cggcggcgtt gacgccggcc cagaagctga acgagcccgc
     2941 gatcacgcag gtggagctgc gacggaagta ggggatgggg ttgagggtgg catcgcgtcc
     3001 gtaccagcct tccatccgcg tgtgatagcc gaccggcttg ttcggcccgg tggcaccgaa
     3061 cgcggaaccg acgccggtat cgaaggtcca gcagctgtcg cgcacggcct cccatcgggc
     3121 ggaatcactc gcccagaagg tgccaccgta gtacatggtg tccacgcccc cgaacgccga
     3181 gaggtgcgag acggatgacg tcgtcccggt ctcggtgccg gcggcgcgct ggaacggctt
     3241 gaggcgcgtg gtggtttgcc cggtgtcgcg ggcctccacg gcgccggcgg ccagcgccag
     3301 cagaaggatg gtcgtgccca gtcttttgcg cattttttcc tccatcgaaa caaacggtgc
     3361 ggacaatggg gaccgggctg gacaactgag ggggtgtttc cagcgggcgt attcgcgggg
     3421 ggacttgcac tgagtatagt gcgcttcgac gcgcatgccg cacgcatgga aaccaattta
     3481 cgactcttta cagtgttgac attgtatgga gttcacgtgc gacgaatgcg agtcgcaatc
     3541 acgcgcgaac atgcgcggcg cgcgcacgac gccacgctca cttgacgtcg cgggaacgcc
     3601 tgtctacgtt gattgggcat cgacccgcgc ccgcaaaccc cgttgtcaac ggggcgacgg
     3661 agtggaaccc aggtatccac ccgatcgccg gaaaggaaac agaagatggc atcccgcgga
     3721 gtgaacaagg tcatcctcgt cggaaacctc ggcgccgacc cggaaatccg ttacacgacg
     3781 agcggcaccg ccgtggccac gctgcgcctg gccaccaccg agtcgtggat ggacaagtcg
     3841 ggcgagcgga ccgaacgcac ggagtggcac cgggtgatcg tgtggggcaa gagcgccgag
     3901 acggccagcc agtacctgag caagggaaag cagctctacg tggaagggcg cctgcagacg
     3961 cgcgaatgga ccgacaagga c
//
LOCUS       DASVOD010000402         4627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_288740, whole genome shotgun sequence.
ACCESSION   DASVOD010000402 DASVOD010000000
VERSION     DASVOD010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288740"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF720_09215"
     CDS             <1..422
                     /locus_tag="VF720_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012611731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7879577.1"
                     /translation="PGLPAVALTVNSSMLTAIGNDFAYEEIFSRQVEALGRPGDCLLA
                     ITTSGNSPNVVEAARAARLLGMTVIGLTGRGGGALAAHCHQLLAVPVADTPRIQEGHI
                     AIGHLLCELVEARLFPPGSTAGRPMARATESDRGPAR"
     gene            424..1326
                     /locus_tag="VF720_09220"
     CDS             424..1326
                     /locus_tag="VF720_09220"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008977.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEX7879578.1"
                     /translation="MLKDLAVEARPWQYTKNFILFAGLVFAHHLLEPAYLVRSLTAFA
                     SFCLLSSFIYVLNDLVDLERDRRHPRKKNRPLASGRLSVGVGVVWCAVLLAGGVALAA
                     WLGKSFLPVATVFVLLNVLYSFVLKEMVVLDVMAIALSFEVRAIAGVEALKALDPGIA
                     ISPWLLVCTLFLALFLGFGKRRHELNLLAADAGDHRPTLAEYSERFLDTLIAIVTAGA
                     MLAYAIYTMSPETVAKFGTTNLVYSIPFVVYGIFRYLYLIYEKKSGGSPSEILLKDFP
                     LALTLAGWVTAVFWVIYRGTPGPA"
     gene            1346..2239
                     /gene="ispE"
                     /locus_tag="VF720_09225"
     CDS             1346..2239
                     /gene="ispE"
                     /locus_tag="VF720_09225"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX7879579.1"
                     /translation="MTFPPGEGLIARAPAKLNLGLWIVRKRADGYHDLVTLFTAVDLY
                     DRLHVRLAPSRITLTTNERRLPTDERNLVVKAAMALAEAADVSLGAEFRLEKRIPTGA
                     GMGGGSSDAAAALLLLDRLWDTRLPRARLARIAADLGSDVPFFLRGGLQLGRGRGTRL
                     TLLKPQAGLEFVVVHGREPIDTGWAYSRYERELTASTPPPSILALAEGSRLSRTTLMH
                     LDNHLEAGLVPDVPDIAERKQLLTSLGARVALLTGSGASVYGLFATRELARRAHLRVA
                     RAGWTADLCRSVSSGVVVEGG"
     gene            2245..2514
                     /locus_tag="VF720_09230"
     CDS             2245..2514
                     /locus_tag="VF720_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HEX7879580.1"
                     /translation="MQITEIRIALRNDDKLKAFVSVTFDDAFVVRGMKIIEGKDGHLF
                     VAMPSRKKPDGTYQDVAHPINVEFRQFLEDQIILEYEKEKVNQPI"
     gene            2616..2690
                     /locus_tag="VF720_09235"
     tRNA            2616..2690
                     /locus_tag="VF720_09235"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2648..2650,aa:Gln,seq:ttg)
     gene            2766..3701
                     /locus_tag="VF720_09240"
     CDS             2766..3701
                     /locus_tag="VF720_09240"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX7879581.1"
                     /translation="METMIKVFSGNANRPLAEGIARSLKRPLGDATVAAFSDGEVNVK
                     IDENVRGNDVFVIQPTNSPASNIIELLVMLDAVRRASPRRVTAVIPYFGYARQDRKDQ
                     PRVAITAKLMANLISTAGADRILTMDLHSPQIQGFFDIPFDHLFASPVFIDHLKSRLP
                     SDFVVVAPDIGSVKMARAYAQRLGAGLALVDKRRPRADEIEVMNIIGDVADRNVVIFD
                     DIISTGSTLVKAALAIKKQGAKKIFAAVTHAVFAPDTSERIQGSELEELVVTDTIHHP
                     PGTLSSKITVLSVGSLLGEAIRRIHEEESLSSLFI"
     gene            3786..4475
                     /locus_tag="VF720_09245"
     CDS             3786..4475
                     /locus_tag="VF720_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX7879582.1"
                     /translation="MAIVTLNGELRGRRGKGAAREARRQGKVPGVLYGKGEEGLPINV
                     GSKELVTVLHGHAGSNVILDVKLAGQESVDRKALIRELQKDPLSGGITHIDLMHISMT
                     EKITVEVPVHVKGLSIGVKDYGGILEFILRTLEVECLPTDIPDEIVVDVTPLMVHDSI
                     HVRDITVPNGKLLNDADQVVVTIVSPQVEKVAAAGEEGAAPAEPEVIGAKKKDDEAGK
                     GEGDGKEKEKK"
     gene            4475..>4627
                     /locus_tag="VF720_09250"
     CDS             4475..>4627
                     /locus_tag="VF720_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879583.1"
                     /translation="MPPAGEPFRIVGLGNPGSRYADTRHNIGFLVIDALAREWRAEPV
                     TTAAPAV"
BASE COUNT          788 a         1600 c         1459 g          780 t
ORIGIN      
        1 gtcccggcct tccggcggtg gcgctcaccg tgaacagctc catgctcacc gccatcggca
       61 acgactttgc gtacgaagag atcttcagcc gccaggtgga ggccctcggt cggccgggcg
      121 actgcctcct ggccatcacc accagcggca actccccgaa cgtggtcgaa gccgcccggg
      181 ccgcccggct cctcggcatg acggtgatcg gcctgaccgg ccggggcggg ggggcgctgg
      241 ccgcccattg tcaccagctc ctggcggtgc cggttgccga cacgccccgc atccaggagg
      301 gacacatcgc catcgggcac ctgttgtgcg aactggtcga ggcgcgtctc ttcccgcccg
      361 gctcaacggc cgggcgcccc atggcccggg cgacggaatc cgaccgggga cccgcccgct
      421 gacatgctga aagacctggc tgtcgaggca cgaccctggc agtacaccaa gaatttcatc
      481 ctcttcgccg ggcttgtgtt cgcccatcac ctcctcgagc cggcgtacct cgtgcggagc
      541 ctcacggcgt tcgcctcgtt ctgcctcctg tcgtcgttca tctacgtgct caacgacctg
      601 gtcgacctcg agcgcgatcg tcgccacccg cgcaagaaga atcgtccgct tgcctccggg
      661 cgcctgtcgg tgggggtcgg cgtggtctgg tgcgccgtgc tcctggcggg cggcgtggca
      721 ctggccgcct ggctcggcaa gtcgttcctc ccggtggcca ccgtcttcgt gctcctcaat
      781 gtcctctatt cgttcgtgct caaggaaatg gtggtcctcg acgtcatggc catcgccctg
      841 tcgttcgagg tgcgggccat cgcgggggtc gaggcattga aggcgctcga ccccggcatc
      901 gccatctcgc cgtggctcct cgtgtgcacg ctcttcctcg cgctcttcct cggcttcggc
      961 aagcggcggc acgagttgaa cctcctggcc gccgacgccg gcgatcatcg ccccacgctg
     1021 gccgagtact ccgagcgctt cctcgacacg ctcatcgcga tcgtcacggc gggggccatg
     1081 ctggcctacg ccatctacac catgagcccg gagaccgtgg ccaagttcgg cacgacgaac
     1141 ctcgtctatt cgatcccgtt cgtcgtctac ggcatcttcc gctacctcta tctcatctac
     1201 gaaaagaaga gcggcggcag tccctccgaa atcctcctca aggacttccc gctggcgctg
     1261 acgctggccg ggtgggtgac cgccgtcttc tgggtcattt atcgcggcac gccgggaccg
     1321 gcatgacagc accagcttgc ccggcgtgac attcccgccg ggcgaggggc tcatcgcccg
     1381 ggcgccggcc aagctgaacc tcgggctctg gatcgtccgg aagcgggccg acggctacca
     1441 cgacctggtc acccttttca cggcggtcga cctgtacgac cggctccacg tccggctggc
     1501 gccctccagg atcaccctga ccaccaatga acgtcgactt ccgaccgacg agcgaaacct
     1561 cgtcgtgaag gcggccatgg cgctggccga ggcggctgac gtgagcctgg gggccgagtt
     1621 ccggctcgag aagcgaatcc cgaccggcgc cgggatgggg ggagggtcga gcgatgccgc
     1681 cgccgcgctc ctcctcctcg accgcctctg ggacacccgg ctccccaggg cccggctggc
     1741 ccgcatcgcc gccgacctcg gttcggacgt cccgttcttc ctgcggggcg gcctgcagct
     1801 cggacggggg cgaggcactc gactcacact cctcaagcct caagccggac ttgaattcgt
     1861 agtcgtccac gggcgtgagc caatcgacac cggatgggct tactctcgat acgaaaggga
     1921 gttgacagct tcgacacccc cgcctagtat cttggcgctg gctgagggga gtcgcctgtc
     1981 gcgcaccacc ctcatgcacc tggacaacca tcttgaagcc ggcctggtgc ccgacgtgcc
     2041 ggacatcgcc gaacgcaagc aacttctgac gtccctgggc gcccgggttg ccttgctgac
     2101 ggggagcgga gcgtccgttt acgggctctt cgccacccgc gaactggctc gacgggcgca
     2161 cctccgggtc gcccgggcag gctggaccgc ggatctgtgt cgttcggttt ctagcggtgt
     2221 cgtggtagaa ggtgggtgag ccggatgcag attaccgaga tccgaatcgc actgcgtaac
     2281 gacgacaaac tgaaggcatt tgtcagtgtc acgttcgacg atgcgttcgt cgtgcggggc
     2341 atgaagatca tcgaagggaa ggacggccac ctcttcgtgg cgatgccgtc gcgcaagaag
     2401 cccgacggca cgtaccagga tgtggcccac ccgatcaacg ttgagttccg gcagttcctg
     2461 gaggaccaga tcatcctcga gtacgaaaaa gagaaggtca accaaccgat ctagcccgac
     2521 gacggctgga tcgattgcgc gatcgaaaga agtcagcgat tccccgatcc acggtcgaac
     2581 ccattccacg acccgcggtc ctctgatcac cgcgttgggg cgtcgccaag cggtaaggca
     2641 gcggcctttg gagccgccat ccggaggttc gaatcctccc gccccaacca cttcgaaatc
     2701 atccgtagcg gtcccgtgac cggttcggct ccttgccgtc cgccgtccgg gacaggagtg
     2761 tcttcgtgga aaccatgatc aaggtcttca gcggcaacgc caatcgcccg ctggccgagg
     2821 gaatcgcgcg ttcgctcaag cgtcccctgg gcgatgccac cgtggccgcc ttcagtgacg
     2881 gcgaggtgaa cgtcaagatc gacgagaacg tccgcggcaa cgatgtcttc gttatccagc
     2941 cgaccaactc gccggcctcg aacatcatcg agctcctggt gatgctcgac gcggtgcggc
     3001 gcgcctcgcc gcggcgggtc acggccgtca tcccgtactt cggctacgcg cggcaggacc
     3061 ggaaggacca gccccgggtg gccatcaccg ccaagctgat ggccaacctc atctccaccg
     3121 ccggcgcgga tcgcatcctc accatggatc tccactcgcc gcagatccag gggttcttcg
     3181 acatcccgtt cgaccacctc ttcgcctcgc cggtcttcat cgatcacctg aagagccggc
     3241 tgccgagcga tttcgtggtg gtggctcccg acatcggctc ggtgaagatg gcccgtgcct
     3301 atgcccagcg cctcggcgcc ggcctcgccc tggtcgacaa gcgccggccc cgcgccgacg
     3361 agatcgaggt catgaacatc atcggcgacg tggccgaccg gaacgtggtc atcttcgacg
     3421 acatcatctc gaccggttcc acgctggtga aggcggccct cgccatcaag aagcaggggg
     3481 ccaagaagat cttcgcggcg gtcacgcacg cggtcttcgc gccggacacg tccgagcgca
     3541 tccagggctc cgagctcgag gagctcgtcg tcaccgacac catccatcac ccgcccggca
     3601 cgttgtcgag caagatcacg gtgctttccg tcggatcgct tcttggtgaa gcgatccgac
     3661 ggatccatga ggaagaatcc ctcagctcgc ttttcattta ggaacgccgg ccttccggca
     3721 ctccgggaag tcacccggcg ccgcgcctcc tggcgcggat catcacgaag ggaaaggaac
     3781 cagacatggc catcgtcacg ctcaatggtg agttgcgcgg ccgccgcggc aagggcgcgg
     3841 cccgcgaagc ccgccgacag ggaaaggtgc cgggagtcct ctacggcaag ggcgaggaag
     3901 ggctcccgat caacgtgggg agcaaggaac tcgtcacggt gctccacggc cacgccggct
     3961 cgaacgtcat cctcgacgtg aagctggcgg gccaggaaag cgtggaccgg aaggcgctca
     4021 tccgtgagct ccagaaggat cccctttcgg gcggcatcac ccatatcgac ctcatgcaca
     4081 tctcgatgac cgagaagatc accgtcgagg tgccggtcca cgtgaaggga ctctcgatcg
     4141 gcgtcaagga ttacggcggc atcctcgagt tcatcctgcg gacactggag gtcgagtgcc
     4201 tcccgaccga catccccgac gagatcgtgg tcgacgtgac gccgctcatg gtgcacgact
     4261 cgatccatgt gcgcgacatc accgtcccca acggcaagct cctcaacgac gccgaccagg
     4321 tggtcgtgac catcgtgtcg ccgcaggtcg agaaggtggc ggccgcgggt gaggaaggcg
     4381 ctgcgccggc cgaaccggaa gtcatcggtg ccaagaagaa ggatgacgaa gccggcaagg
     4441 gcgagggcga cggcaaggag aaggagaaga agtgatcccc cccgccggcg aaccgttccg
     4501 gatcgtcggc ctggggaatc ccgggtcccg ctacgcggac acccgtcaca acatcggttt
     4561 cctggtgatc gacgcgctgg caagggaatg gcgcgccgag cccgtgacca cggcggcccc
     4621 ggccgtc
//
LOCUS       DASVOD010000403         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_132462, whole genome shotgun sequence.
ACCESSION   DASVOD010000403 DASVOD010000000
VERSION     DASVOD010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132462"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VF720_09255"
     CDS             complement(<1..1039)
                     /locus_tag="VF720_09255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX7879584.1"
                     /translation="MRPVVIVDGVRTPFIKSGTEFADVPARELGRAALRELIDRTAVD
                     GGRLDEIIVGNVAGPADATNIARVTALLAGIPQRVPAVTVNRNCASGIQSVADAALRI
                     GAGTAELIAAGGTESMSMVPVFYAESYKQKLFAKGRARSAPARAAAALKFRAADFKPV
                     IALEVGLTDDTCGLNMGDTAEVLAREFGITREAQDRFALQSHERAMAAREQGRLDGEV
                     LPFAVPPRFDRIVSRDNGPRANQTLEALGKLKPYFDRKYGTVTVGNSCPVTDGAAMVL
                     VASEEAAARLGRPVLGRIRSCVFAGLDPSRMGLGPAFAVPAALEKAGVQLADIELIEF
                     NEAFAAQVLANA"
     gene            1645..>2401
                     /locus_tag="VF720_09260"
     CDS             1645..>2401
                     /locus_tag="VF720_09260"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEX7879585.1"
                     /translation="MTITTLSVRAARRLALARSGFLKPDWTDLPTSAGRGAAAARRAA
                     HRIIDRFGYLQLDTVSVAGARSHAIVLLSRLDRFDPDLGEQLLQPGEPLFEYWGHEVC
                     WLPMSMYPDMGWRRERFRHHPWWGDVIGRNPDLARDIMSRVRNDGPLKSSDLEGPSGK
                     GWWNHKPAKNVAVALWTAGDLVVRERKGFQRTFDLPERVIPADIRERPELTLADALPR
                     LLLRAIDGHGWAQTGTLAQSWRLRNLKSEIQAAL"
BASE COUNT          398 a          819 c          784 g          400 t
ORIGIN      
        1 cggcattggc caggacctgt gcggcgaacg cttcgttgaa ttcgatcagc tcgatgtcgg
       61 cgagctggac gccggctttc tcgagcgccg ccggcaccgc gaacgccggc cccagcccca
      121 tgcgcgacgg gtcgagcccc gcgaagacgc acgaccggat tcggccgagg acggggcggc
      181 ccagccgcgc cgcggcctcc tcggaggcca ccagcaccat ggcggcgcca tccgtgaccg
      241 ggcacgagtt gccgactgtg accgtgccgt acttacgatc gaaatacggc ttcagcttgc
      301 cgagcgcttc cagcgtctgg ttggcgcgag gaccattgtc gcggctcacg atccggtcga
      361 agcggggcgg cacggcgaac ggcagcacct cgccgtcgag gcgcccctgc tcacgggcgg
      421 ccatggcccg ctcgtgactc tggagcgcaa accggtcctg tgcctcgcgc gtgatgccga
      481 actcgcgggc cagcacttcg gccgtatcgc ccatgttcag gccgcaggtg tcgtcggtga
      541 gccccacttc gagggcgatg accggcttga agtccgcggc gcggaacttg agcgccgccg
      601 ccgcgcgggc cggggccgag cgggcccgcc ccttggcgaa gagcttttgc ttgtacgatt
      661 cggcatagaa gaccggcacc atcgacatcg actcggtgcc cccggcggcg atcagttccg
      721 ccgtgccggc tccgatgcgg agcgcggcat cggctacgct ctggatgccc gaggcgcagt
      781 tccggttgac ggtgacggcg ggcacgcgct gcggaatgcc ggccaggagc gccgtgaccc
      841 gggcgatgtt ggtggcatcc gccgggccgg ccacgttgcc gacgataatt tcgtcgagcc
      901 ggccgccatc gaccgcggtg cgatcgatca gctcgcgcaa cgcagcgcgg ccgagttccc
      961 gggccggcac gtcggcgaat tcggttccgg acttgatgaa cggcgtacga acaccgtcga
     1021 cgatgacaac cggtcgcatg atgcgcggat ggtagcgtcc gactcgctca aagtcgaacc
     1081 gcgcgcgatc gcgtgagata ggtgaacccg gccgtcgctc tctcttcgac agccgggttc
     1141 gttgatgggg gaccgcaatt catgatcacg atctcccgaa gctggtgcgg ggccggtgag
     1201 ccaaccgacg tccgcggtca acgagagagg attcactgtt tcgtctcgcg cgttcctcct
     1261 gcctcgagag agcagacgta caggccggag gcccgggcct taccgtcgtc gtggcaggct
     1321 cacattgcat gggtggggcc agacggaagg caaatccgca agtcattgtc ccgattaagg
     1381 ttgcctatcc tgatcgatca agtcgtgtca agaaatatga acgaaccgct caccagccac
     1441 accatctcag atctcttaac actattattt agagacattt aacctggcga caacccacgt
     1501 tgacttccag gggcgcccgt taacgatccc gaacacacct gggcctcctc attagatagg
     1561 cgcaggccgg tttcccgagg gcctcgcggg gcgctactct ccccccgccc gcgtgacccg
     1621 ctggctccac ttcccgggaa cgacatgacg atcacgactc tttccgttcg ggccgcgcgc
     1681 cggctggcgc tggcccgttc cggttttctg aagcccgact ggaccgacct tcccaccagc
     1741 gccggacgag gcgcggccgc cgcgcgacgc gccgcccacc gcatcatcga tcgctttggt
     1801 tacctgcagc tcgacaccgt gtcggtggcc ggggcccgca gccacgcgat cgtccttctc
     1861 tcgcgactcg atcggttcga tcccgacctc ggcgagcaac tgctccaacc gggggagcct
     1921 ctcttcgaat actggggcca cgaagtctgc tggctgccga tgtcgatgta cccggacatg
     1981 ggttggcgtc gcgaacgctt ccggcatcac ccctggtggg gcgatgtgat cggcaggaac
     2041 cccgacctcg cgcgcgacat catgagccgc gtccggaacg atggcccgct caagtcgagc
     2101 gatctggagg gtccatcggg caagggttgg tggaatcaca agccggccaa gaacgtggcc
     2161 gtggccctct ggacggccgg tgacctcgtc gtgcgggagc ggaagggatt ccagcgcacg
     2221 ttcgatctcc ccgagcgcgt gataccggcc gacatccgcg aaaggccgga gctgaccctg
     2281 gccgacgcgc tcccccgtct ccttcttcga gcgatcgacg gacatggttg ggcgcagacc
     2341 ggcacgctcg cgcagagctg gcgcctgcgc aatctcaagt cggaaatcca ggcggcactc
     2401 g
//
LOCUS       DASVOD010000404         2424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_548560, whole genome shotgun sequence.
ACCESSION   DASVOD010000404 DASVOD010000000
VERSION     DASVOD010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548560"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VF720_09265"
     CDS             complement(<1..425)
                     /locus_tag="VF720_09265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX7879586.1"
                     /translation="MATLTRLPIVSLVGRPNVGKSTFFNRIVGGTRAVVHDLPGVTRD
                     RNEAETRWREIPFTLVDTGGLVPNAKSGMTAAIRRQTEVAFKEADLILFLVDGIEGPT
                     AIDREIAEAIRASGQPYLFVVNKIDTPRAEADAWTFGDLG"
     gene            complement(425..1729)
                     /locus_tag="VF720_09270"
     CDS             complement(425..1729)
                     /locus_tag="VF720_09270"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEX7879587.1"
                     /translation="MQIRAIREGSTAARSPLRPGDFVRRVNGVPVTDFLDLYFHLSEE
                     EVKLDVERPGEGSQATRFQVALTRSYGEEFGLEFPETKFRVCGNKCPFCFVDQNPAGM
                     RKAVYVRDDDYRFSFLDGHFITLTNLKESDFQRIIDYHLSPLYVSVHALDPEVRTYLL
                     GTARARMIRPQIERLLGHGIQLHTQIVALPGVNDGAVMDDTIDGLFSYYPGVQSIGVV
                     PIGLTAHMPRERGLRPYTQEEAAAVCDHVQRRHDGFRARIGKGFVYMADEFLLLAGKP
                     IPTTPYYDGFIQLGNGVGMVRELMNDFRKRTLDTAGLFRRGLRQVVVLSGESAGPTVA
                     RMLSKKVPAKAGITIDTRVVVNETYGRPTSVTGLLGGRDFERELHAAGSFDLALVPPN
                     ALNDRGLFIDDVPFDDLNARFGGRLSVGFDSLWAASARESAA"
     gene            complement(1757..1832)
                     /locus_tag="VF720_09275"
     tRNA            complement(1757..1832)
                     /locus_tag="VF720_09275"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1797..1799),aa:Gly,seq:ccc)
     gene            complement(1881..>2424)
                     /locus_tag="VF720_09280"
     CDS             complement(1881..>2424)
                     /locus_tag="VF720_09280"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEX7879588.1"
                     /translation="MEGSHVLHAELAALDPGSAERLHRNDAQRITRALEVVLLTGAPM
                     GTLWEAGESPSPLAARCRLLVLERPREEIYARIERRTGELLSGGMIEEAARVLASGVD
                     PGSPTFKAHGYPEIARHLAGDIDRDELTRRLNQVTRNYAKRQMTWFRRLPGALSVPVS
                     GEESPAETAAKALARLEDIT"
BASE COUNT          387 a          783 c          849 g          405 t
ORIGIN      
        1 cccaggtcgc cgaacgtcca ggcgtcggct tcggcgcgcg gcgtgtcgat cttgttgacg
       61 acgaagaggt acggctggcc gctggcgcgg atggcctcgg cgatctcccg gtcgatggcg
      121 gtgggccctt cgatgccgtc gacgaggaag aggatgaggt cggcctcctt gaaggccacc
      181 tcggtctgcc gacggatggc ggcggtcatc ccgctttttg cgttcggcac gaggccgccc
      241 gtgtcgacga gcgtgaacgg gatctcgcgc cagcgcgttt cggcctcgtt ccggtcgcgc
      301 gtgacacccg gcaggtcgtg cacgacggcc cgcgttccgc ccacgatccg gttgaagaag
      361 gtggacttgc ccacgttcgg acgcccgacg aggctgacga tcggaaggcg ggtgagcgtg
      421 gccatcaggc cgccgattcc cgggccgagg cggcccagag agagtcgaaa ccgacgctca
      481 gccggccacc gaagcgggcg ttcaggtcgt cgaacggcac gtcgtcgatg aagaggcccc
      541 ggtcgttgag ggcgttcggc ggcacgaggg ccagatcgaa cgaaccggct gcgtggagct
      601 cccgctcgaa atccctgccg ccaaggaggc ccgtgaccga agtcggccgg ccgtaagtct
      661 cgttgacgac cacgcgcgtg tcgatggtga tgccggcttt cgccggcacc tttttcgaga
      721 gcatgcgagc cacggtggga ccggcactct ctccggagag gacaacgacc tgtctcaggc
      781 cacggcggaa gagccccgct gtgtccagtg ttctcttgcg gaaatcattc atgagttccc
      841 gcaccatccc cacgccgttc ccgagctgga tgaagccgtc gtaatacggc gtggtgggga
      901 tcggcttgcc ggccaggagg aggaactcat cggccatgta gacgaacccc ttcccgatcc
      961 tggcccggaa tccgtcgtgc cgtcgctgga catggtcgca gacggccgcg gcctcttcct
     1021 gcgtgtacgg gcgcaggccg cgctccctgg gcatgtgggc cgtgaggccg atcggcacga
     1081 cgccgatcga ctggacgccc gggtagtagg agaagagacc gtcgatggtg tcgtccatga
     1141 ccgcgccgtc gttcacgccg gggagcgcca cgatctgggt gtggagctgg atgccgtggc
     1201 cgaggagccg ctcgatctgc gggcggatca tccgggcgcg ggccgtgccg aggaggtagg
     1261 tgcgcacctc cgggtcgagg gcatgcaccg acacgtagag gggcgagagg tggtagtcga
     1321 tgatccgctg gaagtcggac tccttgaggt tcgtcagcgt gatgaagtgg ccatcgagga
     1381 acgagaagcg gtagtcatcg tcccgcacgt aaacggcctt ccgcatgccg gccgggttct
     1441 ggtcgacgaa gcagaagggg cacttgttgc cgcacacccg gaacttcgtc tcggggaatt
     1501 cgaggccgaa ctcctcgccg taggatcggg tgagggccac ctggaagcgg gtggcctggc
     1561 tcccctcccc ggggcgctcc acgtcgagct tcacttcctc ctcggaaagg tggaagtaga
     1621 ggtcgaggaa gtcggtcacg ggcacgccat tgacccggcg gacaaagtcc cccgggcgga
     1681 gtggcgacct ggccgccgtg ctcccctctc ggatcgcgcg aatctgcatg ccactccgac
     1741 ttcccggcgg gctaattgga gcgggcaacg gggttcgaac ccgcgacatt cagcttggga
     1801 agctgacact ctaccaactg agttatgccc gctctctgac ttcgtaacag gctggagatt
     1861 atccgcttgc cggatttgcg tcaagtaatg tcttcaaggc gcgcgagcgc tttcgcggcc
     1921 gtttccgcgg gcgattcctc ccccgaaacc gggaccgaca gcgcccccgg aaggcggcga
     1981 aaccaggtca tctgccgctt ggcgtagttg cgggtgacct ggttcaggcg ccgtgtcaat
     2041 tcgtcccggt cgatgtcgcc cgccaggtgc cgggcgattt cgggatagcc gtgggccttg
     2101 aacgtgggcg acccggggtc gacacccgag gcgaggacgc gcgcggcctc ctcgatcatc
     2161 ccgcccgaca ggagttcacc ggtccggcgt tcgatgcggg cgtagatctc ctcgcgcggc
     2221 cgctcaagga cgagcaaccg gcaccgcgcg gccagcggcg acggcgactc acccgcctcc
     2281 cagagagtcc ccatcggcgc gccggtcagg aggacgacct cgagcgcccg ggtgatccgc
     2341 tgggcatcgt tcctgtgcag ccgttccgcc gacccgggat cgagtgccgc gagctccgcg
     2401 tgcaggacgt ggctcccctc catg
//
LOCUS       DASVOD010000405         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_289024, whole genome shotgun sequence.
ACCESSION   DASVOD010000405 DASVOD010000000
VERSION     DASVOD010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289024"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="prfB"
                     /locus_tag="VF720_09285"
     CDS             <1..999
                     /gene="prfB"
                     /locus_tag="VF720_09285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX7879589.1"
                     /translation="VSALEEKMAAPDFWNKPEAARQVIAELKVAKTILADWTSLDAEA
                     RTVSELLDLAQAEGDESTLKDLESEGARLSARLEALELRSFLSAPEDSASAIVSIHPG
                     AGGTESQDWASMLLRMFTRWCERNGFETNFLDYQDGDEAGIKDASFEVNGEYAYGYLK
                     GESGVHRLVRISPFDAAKRRHTSFASIFVLPQVEAAADIEVNEGDLRVDTFRASGAGG
                     QHVNKTSSAVRLTHIPSGIVVSCQSERSQHRNRESAMKILKARLYMKRREEEQKKMDK
                     LIDEQKDIAWGSQIRSYVFQPYTMVKDHRTDHQTSDVGKVMDGDLNPFIDAWLRKR"
     gene            1031..2407
                     /locus_tag="VF720_09290"
     CDS             1031..2407
                     /locus_tag="VF720_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX7879590.1"
                     /translation="MMNSNIFREYDIRGIAETDLGDADVTIIGRALGTFYARHGAKTV
                     VVGQDVRLTSPRISKALIAGLNASGISVVTIGTVPTPAMYYAVHAWNMDGGVAVTGSH
                     NPVEFNGFKMNLGKASIYGTMIQEIRAIAEKGDFASGAGTVSERDVLAEYNAVLRRKN
                     HLSRPLKVVIDAGNGCAGPHAPAFFRDMGCTVEELYTEPDGRFPNHLPDPTVPALIKD
                     LVALVQSSGADLGIGYDGDADRVGLVDNTGRVVWGDQLLAIAAADMLTRKPGAKVIFE
                     VKCSQAVADWVTKHGGVPILWKTGHSLIKAKMKEEGALLAGEMSGHMFFAEDWYGFDD
                     ALYASARLASIVADSGKSLAALVDELPKYYSTPELRFECDDTAKFDVVRTVVEHFRRE
                     HEVIDVDGARILFGDGWGLVRASNTQPVLVARFEARSPERLEAIKSAVMDYLATFPAV
                     HLDKLAGH"
     gene            2412..3077
                     /locus_tag="VF720_09295"
     CDS             2412..3077
                     /locus_tag="VF720_09295"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX7879591.1"
                     /translation="MTRLTARDRRPMSLAWHRRPAVDLARALLGKLFIHRTRDGEAGG
                     WIVETEAYVGEEDPACHAAAGRTDRTEVMYGLAGHAYVYFTYGMHWCANVVAARSGRP
                     EAVLIRALEPAVGLPLMRRRRGGEVPDRELARGPARLCVALGINGLDNGRRLDSGPLM
                     VTAGRPGPRILGVSARVGIRVGTDRPWRYYDAESNFVSPGRPGPPSGRRSLLAVDTLA
                     AGR"
     gene            3163..>3559
                     /locus_tag="VF720_09300"
     CDS             3163..>3559
                     /locus_tag="VF720_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879592.1"
                     /translation="MKPSPSSCVLVTVGLAVALLIGCEGGVDQLQPGAIDDNELVAAY
                     VDPLTPTPGVNPRTGGDGDLLPVIDGDAQDKEWEVARPLYVYVSADRAVGGRGFYVEL
                     RSVWSDESRYAGAVNHVYFLVRWSDDTLDN"
BASE COUNT          613 a         1184 c         1187 g          575 t
ORIGIN      
        1 gtaagcgccc tcgaggaaaa gatggccgcg cccgacttct ggaacaagcc cgaggcggcc
       61 cggcaggtca tcgccgaact gaaggtggcc aagacgatcc tggccgactg gacgagcctc
      121 gacgccgagg ctcgcacggt gagcgagctc ctcgacctgg cgcaggccga gggggatgag
      181 tccaccctca aggacctcga gtcggaaggg gccaggctct cggcccgcct cgaggcgctc
      241 gagctccgtt cgttcctgtc ggctcccgag gattcggcca gtgccatcgt gtcgatccac
      301 ccgggcgccg gcggtaccga aagccaggac tgggcgtcga tgctcctgcg catgttcacc
      361 cgttggtgcg agcgcaacgg cttcgagacc aacttcctcg actaccagga cggcgatgag
      421 gccggcatca aggatgcctc gttcgaagtg aacggcgaat acgcctacgg ttacctgaag
      481 ggggagagcg gcgttcaccg cctggtgcgc atcagcccgt tcgacgccgc caagaggcgt
      541 catacctcgt tcgcctcgat cttcgtcctg ccccaggtgg aggcggccgc cgacatcgag
      601 gtcaatgaag gggatctccg cgtggatacc ttccgggcct cgggcgccgg cgggcagcac
      661 gtcaacaaga cgagctcggc ggtgcgcctc acgcacatcc cgtcggggat cgtcgtgtcg
      721 tgccagagcg agcgaagcca gcatcgcaac cgtgaatcgg ccatgaagat cctcaaggcg
      781 cggctgtaca tgaagcgtcg cgaggaagag cagaagaaga tggacaagct gatcgacgag
      841 cagaaggaca tcgcctgggg aagccagatc cgctcgtacg tcttccagcc ctacacgatg
      901 gtcaaggacc atcgcaccga tcaccagacg tcggatgtcg gcaaggtgat ggacggggac
      961 ctgaacccgt tcatcgacgc ctggctccgc aaacgataga ccgccgcgcc cgcggagggc
     1021 gcctggagac atgatgaact cgaacatctt ccgggaatac gacatccgcg gcatcgccga
     1081 gaccgacctg ggcgacgccg acgtcacgat catcggccgc gccctcggca ctttctacgc
     1141 tcgccacggc gcgaagacgg tggtggtggg acaggacgtc cgcctcacct cgccgcgcat
     1201 ctcgaaggcc ctgatcgccg ggctcaatgc ctcgggcatc agcgtcgtca ccatcggtac
     1261 cgtgccgacg ccggccatgt actacgcggt gcacgcctgg aacatggacg gtggcgtggc
     1321 cgtcaccggc agccacaacc cggtggagtt caacggcttc aagatgaacc tgggaaaggc
     1381 ttccatttac gggaccatga tccaggagat ccgcgccatc gccgaaaagg gggacttcgc
     1441 ctcgggggcc ggtaccgtaa gcgaacgcga cgtgctcgcc gagtacaacg cggtgctccg
     1501 caggaagaat cacctgagcc ggccgctcaa ggtggtcatc gacgcgggga acggctgcgc
     1561 cggtccgcac gcgccggcct ttttccgcga catggggtgc acggtcgagg agctgtatac
     1621 cgagcccgac ggtcgcttcc cgaaccatct tcccgatccg accgtgccgg ccctcatcaa
     1681 ggacctggtg gcgctggtcc agtcgtccgg agccgatctc ggcatcggct acgacggtga
     1741 tgccgaccgg gtcgggcttg tcgacaacac cggtcgtgtc gtctggggcg accagctcct
     1801 tgccatcgcg gcggccgaca tgctgacccg caagccgggc gccaaggtga tcttcgaggt
     1861 gaagtgcagc caggccgtgg ccgactgggt aacgaagcac ggtggcgtcc cgatcctctg
     1921 gaagaccggc cactcgctca tcaaggccaa gatgaaggaa gagggagcgc tgctggccgg
     1981 cgagatgagc ggccacatgt tcttcgccga ggattggtac ggcttcgacg atgccctcta
     2041 cgcgtcggcg cgcctcgcgt cgatcgtggc cgattccgga aagtcgctgg cggccctcgt
     2101 cgatgagctc ccgaagtact actccacgcc ggagctccgc ttcgagtgcg acgacaccgc
     2161 caagttcgac gtcgtgcgga cagtggtcga gcacttccgc agggagcacg aggtcatcga
     2221 cgtcgatggc gcccggatcc tcttcggcga cggctggggt ctggtccggg cctcgaatac
     2281 gcagcccgtg ctcgtggccc gtttcgaggc cagatcgccc gagcggctgg aggccatcaa
     2341 gagcgcggtc atggactacc tcgccacctt cccggccgtc catctcgaca agctcgcggg
     2401 ccactgaggc gatgacacgc ctgacggcgc gcgatcgccg gccgatgtcc ctggcctggc
     2461 atcgccgacc ggccgtcgac ctcgcgcgcg cgctgctcgg caagctcttc atccaccgga
     2521 cgcgcgatgg ggaggccggc ggctggatcg tggagaccga ggcctacgtg ggcgaggaag
     2581 acccagcctg ccacgcggcg gccggtcgca ccgatcggac cgaggtcatg tacggccttg
     2641 ccggtcatgc ctatgtctac ttcacttacg gcatgcactg gtgcgccaat gtggtggcgg
     2701 cgcggtcggg gaggccggag gcggtcctca tccgggcgct cgagccggcg gtgggccttc
     2761 cgctcatgcg tcgtcgccgg ggaggggagg tgccggatcg cgaactggcc cgcggtccgg
     2821 cccgtctctg cgtggcgctc gggataaacg gcctggacaa cggccggcgc ctcgactcag
     2881 gccccctcat ggtgacggcc gggaggcccg gtccgcggat cctcggcgtt tcggcccggg
     2941 tcggcatccg ggtggggacg gatcgcccct ggcgttacta tgacgctgaa agtaatttcg
     3001 tcagccccgg tcggccgggc cccccgtcgg gaaggcgttc gctgttggcg gttgacactc
     3061 tcgcggccgg ccgctaactt atttctctaa caccagacac tcggtccgcg atctccctcc
     3121 tcacctgtcg cgtcacccat cattaataag gatgacatac agatgaagcc atcaccgtca
     3181 tcgtgcgttc tcgtgacggt cggcctggcc gtcgcgctgc ttatcggttg cgagggaggg
     3241 gtcgaccagc tccagccggg ggccatcgac gataacgaac tcgtagcggc ctacgtcgat
     3301 ccactcaccc ccacgccggg agtcaatccg cgcaccggcg gagacggcga cctcctcccc
     3361 gtcatcgacg gggatgccca ggacaaggaa tgggaggtgg cccgccccct ctacgtgtac
     3421 gtcagtgccg accgggcggt cggcggccgc ggtttctacg tggagctccg ctcggtctgg
     3481 tcggacgaat cgcggtatgc cggtgcggtc aaccatgtct acttcctggt ccgctggtcc
     3541 gacgacacgc tggacaacc
//
LOCUS       DASVOD010000406         9946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_497555, whole genome shotgun sequence.
ACCESSION   DASVOD010000406 DASVOD010000000
VERSION     DASVOD010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497555"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2624
                     /locus_tag="VF720_09305"
     CDS             <1..2624
                     /locus_tag="VF720_09305"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879593.1"
                     /translation="KLAEVEALLAADAALESGADDFLDRPIDAGRLMAAEVEVPSYAG
                     RTLGGYRITRKIGEGGMGVVYEAEQESPRRRVAVKVVRGGAFADGERLRFFQREAHAL
                     ARLKHPGIAAIYEAGVGDGGDHFFAMELVQGVPLDAWIKARNSTGPLDRMEIRERLSL
                     YLEIAAILAYAHQRGVIHRDLKPSNILVMDEPPGDSASTARFRVKLLDFGLARLTDAD
                     APIDANPDFPADVRSATHAIHGTLAYMSPEQARGSRDDIDSRSDVYALGVLLYEMLTG
                     RLPYEVKRGHLFESLEIIERTPPRPPSDSCRLLAGDLETILYKALEKDPERRYPSVSA
                     FADDVQRYLANLPIQARPPTLAYQLTKLVRRHRTVAILAGVLLLAVLAGAAGTTVGML
                     KARESARLAREEAATAEQVSTFLERLFRVSSPSEARGNAITARELLDAGVARIDTSLA
                     DQPRVKGRLLGVMGEVYRSLGLYKEARPLLERSLVLRRSTLPADDPAVAQNEYVLGGL
                     LRRLGDYDGARAHYERALALREERYGPDHPEVAVSLAGLANVMLETEDYEGARPLYER
                     CIAIIEKHKGVDDPSLTIYLGNLAILHRATNGYDKALPLVLRALAISEKALGPDHPSL
                     GYDVIGLASTYHRLGRYDEARSAYRRALAIQEKNFGPTHTDVAETLMGLGLLEQETGH
                     AEQALPVMERSVAIFEAALGRENPYTARSLKNLALVLSELGRHEEAIRMGREAIRTFE
                     SAASPEPAQTAMARINMGRILLAAGQPRESAEILALGMNEGAKSLGDEDYYVMEMAKW
                     RCLALDAAGRARESARLRAEILAALDPLPDWADTTFTATFDDAAAAFRASGQPDSARV
                     VEEARRLFLERLARTTS"
     gene            complement(2654..2947)
                     /locus_tag="VF720_09310"
     CDS             complement(2654..2947)
                     /locus_tag="VF720_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879594.1"
                     /translation="MTTEFEGARRTALDRMEQAERNYRMAFIVAVLIEMLFLVGFLLL
                     ADFSNRTHVLLLIATVSIYTITAFGLLALGAHVQRNTLRLLKAVELLSGPVGK"
     gene            complement(3082..3630)
                     /locus_tag="VF720_09315"
     CDS             complement(3082..3630)
                     /locus_tag="VF720_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7879595.1"
                     /translation="MTDNGRQEAFWVLRAQSGDREAVDRLLRGVEGPLGRYLSGLVGN
                     RAPAEDLLQDVLTIVYRKLRWLQDPALFRPWVFRIASREAFKRLKRERRWSEQVRDPE
                     VLDAVPDESGTPATMVPGRFGGLMAGLSPASRAVLVLHYHEGMTLTEIADVLEIAPGT
                     VKSRLAYGLAALRQTLGPHEAR"
     gene            complement(3712..5787)
                     /locus_tag="VF720_09320"
     CDS             complement(3712..5787)
                     /locus_tag="VF720_09320"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX7879596.1"
                     /translation="MKSIVTLLACPVLALLASGCSQPKPADKPAAQAAPKVTADTYLA
                     QFVDTTVTPSDDFFHFAVGGWLKRHPIPESERSWGIGKVVQEETYHRLLEINQEAAVR
                     TDAAPGNNAQKIGDFWAAGMDTAAIDSAGYKPLQAEFDRIDAITDRKSLLDVIARLQY
                     IGAGPLYGQYIFQDEKDSEHEVLHLYQGGLGLPNRDYYVDPDARSTHIRREYVAHVAK
                     MLQLMGDTPEAAKKASETIMRLETDLAKASRKLEDLRDPEANYNPMTVATLARLTPSI
                     DWKAMLEAGNIRDIDKVVVGQPEFFRQVEKSLRTHSIDEWKTYLRWNLANTFADAAGG
                     AFDRQNFQFYETVLAGTKTQRPRWKRVLDAEENYLGDALGELYVERYFGPETKLRYAE
                     LTNKVFDAFRARIHNLTWMSEATRERALKKLSSVTVKVGYPDKWRDYSNYPVSRESHL
                     KNVMYGNIWASEYAIAKLHKPVDRTEWDMTPQTYNAYYNPSNNEIVMPAAAFILPGID
                     DQEVDDALVYGYAGGTTIGHEITHGFDDDGRQFDEKGNLEDWWTKEDAAEFEKRAERI
                     VAQFNDYVVLDSLHVNGKATSGENIADLGGMLLAWDAFTQTAQYKEGKKLGGFTPAQR
                     FFIGWSLGWMNHFRPETQALRIKTDVHSPAFLRVNGPMANLPQFYEAWNVKPGDRMHR
                     ADDVRVSIW"
     gene            5993..6946
                     /locus_tag="VF720_09325"
     CDS             5993..6946
                     /locus_tag="VF720_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879597.1"
                     /translation="MKRREYGLFLLAAVILLLPGMAHAASIHDARFFFHLAAPVGKNP
                     CSLRSNPPACNDVVTKGQVGPGYFAYIVISNTDATGGLNSGKFGIDYNGTAGAGVDIF
                     SWTNCSSIDFPSPGWPNADTGTILTWDAASCHHAVASGTVMAIGGYFYLSAYTPDEIE
                     MTPRPLDNRLEVSECNGAVVNLDPTLQNLGWARFTDTGAAEGHAPCNFRNSPCRVRGP
                     QTVAAGQGGINFTVDPEYVAPLGHWIIQGNGQISSSNNASAVVQALGAGSFTLTYRIS
                     TGCAEGCQCDYPVNVVGTAGVPAAPPDTSPTTWSRVKGAYR"
     gene            7101..8147
                     /locus_tag="VF720_09330"
     CDS             7101..8147
                     /locus_tag="VF720_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879598.1"
                     /translation="MTLARSVRRFVPVLLAPVILLAPVPPALAAGEITVAKDTIRPKM
                     DARGCMMDDKGCKGGDLIAYPGLIAELNGALPSGAQPYVEFRMNGKSIKIDAGSDDVY
                     GIDNRTKIFVTDNHGSENGWVFPGKIPAGTVEFTIGVRNELTESNDVIYQGKFDYEKR
                     LISAGNEETVELLVNDDWRLPIGYLYMTKDRGLHVVTWYRGRPGGVKTFLFRDGVEVA
                     KNEGCGIGDVADFDPTHYMYWEVDCEITGVYGDEESAKNGYEPNINLAANPGNYEFKC
                     LAGGKLARVIKFTVNADGSFDNGIATANQLGSERVIVPVEVKMDNPTWNKAAWKTGAY
                     YGHPLTGFAVPGQP"
     gene            8207..>9946
                     /locus_tag="VF720_09335"
     CDS             8207..>9946
                     /locus_tag="VF720_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7879599.1"
                     /translation="MFCCILLAVALLFGARVAGARPWYNANFDGYSLASGSRIFPYNA
                     NNDAELDIITSSGNVILGMPNGDFSQGVGFTIPLSTYHMAIADQNGDGRKDVFLTGKP
                     TPGAWGTFYLYTYQKLAGNGFTALPGIVLPEKSDVLHAGDFDGNGLADAVVFTRGLDF
                     PDVPDTGAVYFSHPGGISPGVVFEIPMLYPIDSGVGDFDEDGRTDIVVLSAFNLNPRT
                     LAFYLSSAGGILAAPRIDTLSTIFPGYIGVADVNDDGHDDIAFSDGRAPASFLGNGDG
                     TFQPVYPPDVGPAWCGDLEFGDVNLDGHLDVVSNCTFNYAYIGLGDGAGRFSTVSRHW
                     FPRGGSEESLIVNDFDHDGFGDIVGNRSPGLAVLRGNAAVTFNTVPEFPAAPNLSEVI
                     ALDWDLDGIEDLVGVDRIGGNLVLFRNRLGALEPGVTIPLGFIPDGIRFADLNGDGRR
                     DIVAADQGADLVKVSLGSSGGGFQTPVSYPVGSDPSDVATGDFNADGILDVVVSNFSY
                     PSWSASYLRGTGNGAFAPQVVIPTAATPSSIETGDFNEDGKLDFVTGHPFDRCSLVLG
                     RGNGTFDPYIGVGT"
BASE COUNT         1666 a         3362 c         3190 g         1728 t
ORIGIN      
        1 ccaaactcgc cgaggtcgag gcgctgctcg ccgccgatgc cgccctcgag tcgggtgccg
       61 acgacttcct cgatcgaccg atcgatgccg gccgactcat ggccgccgag gtggaggttc
      121 cctcgtacgc cggccgcacg ctcggtggct accgcatcac gagaaaaatc ggcgaagggg
      181 ggatgggcgt cgtctacgag gccgagcagg aatcgccgcg gcggcgggtg gcggtcaagg
      241 tggtacgcgg cggggcgttc gccgacgggg agcggctccg gttcttccag cgcgaggcgc
      301 atgcgctggc acggctcaag catcccggca tcgccgccat ctacgaggcc ggtgtcggtg
      361 acggtggcga ccatttcttc gcgatggagc tggtgcaggg agtgccgctc gatgcctgga
      421 tcaaggcccg caattcgacc ggcccgctcg atcggatgga aatccgcgag cgcctctccc
      481 tctacctcga gatcgccgcc atcctggcct atgcccacca gcggggcgtc attcaccggg
      541 acctgaagcc ctccaacatt ctcgtgatgg atgaaccccc gggcgattcc gcctccaccg
      601 cccgcttccg ggtgaaactc ctcgatttcg gtctggcgcg cctcaccgat gccgacgcgc
      661 ccatcgacgc caaccctgat tttccggccg acgtccgctc ggccacgcac gccatccacg
      721 gcacgctggc ctacatgagt cccgagcagg cccgcggctc ccgcgacgac atcgattccc
      781 ggagcgacgt ctacgcgctg ggcgtgctcc tctacgagat gctgaccggc cgcctcccct
      841 acgaggtgaa gcggggccac ctcttcgaat cgcttgagat catcgagcgc acgccgcccc
      901 gcccgccttc cgattcgtgc cggctcctgg ccggtgatct cgaaacgatc ctgtacaagg
      961 ctctcgagaa ggaccccgaa cggcgttacc ccagcgtgag cgccttcgcc gacgacgtgc
     1021 agcgctacct tgccaacctg cccatccagg cccgcccccc cacgctggcc taccagctca
     1081 ccaagctcgt ccgccgccac cgcaccgtgg ccatcctggc cggcgtgctc ctcctggctg
     1141 tcctggccgg cgcggccggc accactgtcg ggatgctcaa ggcgcgcgaa tcggcccggc
     1201 tggcgcgcga agaggccgcc accgccgagc aggtttccac gttcctcgag cgcctcttcc
     1261 gcgtatcgag cccgagcgag gcgcgcggca acgccatcac cgcgcgtgag ctcctggatg
     1321 ccggcgtggc ccggatcgac accagcctgg cggaccaacc gcgcgtcaag ggacgcctcc
     1381 tcggcgtgat gggagaggtg taccgcagcc tcggcctgta caaggaggca cggcccctcc
     1441 tcgaacgatc gctggtcctg cgccgctcaa cgctccccgc cgacgacccg gccgtggcgc
     1501 agaacgagta cgtcctcggc gggctgctcc gccggctcgg cgactacgac ggcgcccgcg
     1561 cccactacga gcgcgcgctg gccctgcgcg aggagcgcta tggtccggat catccggaag
     1621 tggccgtcag cctggccggc ctggccaacg tcatgctgga aaccgaggac tatgaagggg
     1681 cgcggcccct gtacgagcga tgcatcgcca tcatcgagaa acacaagggt gtggacgatc
     1741 caagcctcac gatctacctc ggaaacctag ccatcctcca tcgcgccacg aacgggtacg
     1801 acaaggcgtt gcccctcgtg ttgcgcgcac tggccatctc cgagaaagcg ctcggaccgg
     1861 atcatccgtc gctcggctac gatgtgatcg gcttggccag cacctatcac cgcctcggcc
     1921 gctacgacga ggcgcgttcg gcctatcggc gggcgttggc catccaggaa aagaacttcg
     1981 ggcccacgca taccgacgtg gcggaaacgc tcatgggcct cggcctgctc gagcaggaaa
     2041 ccggccacgc ggagcaggcg cttccggtca tggagcgatc ggtggccatt ttcgaagcgg
     2101 ccctcggccg tgagaatccc tacaccgccc gttcgctcaa gaacctggcc ctggtgctgt
     2161 cggaactcgg acggcacgag gaagccatcc ggatgggacg tgaggccatc cggacattcg
     2221 agtcggcagc cagtcccgaa ccggcgcaga ccgccatggc ccgcatcaac atgggcagga
     2281 ttctgctggc ggccgggcag ccacgcgagt cggcggaaat cctggcgctc ggcatgaacg
     2341 agggcgccaa atcgctgggc gatgaggact attacgtgat ggaaatggcc aagtggcgat
     2401 gcctggcgct cgatgcggcc ggccgggcgc gcgagtccgc ccgactgcgc gcggaaatcc
     2461 tcgcggcgct cgatcccttg cccgactggg ccgacacgac gttcaccgcc accttcgatg
     2521 acgcggccgc ggcgttccgt gcgagcgggc agccggacag cgcgcgggtg gtcgaggaag
     2581 cccggcgcct cttcctcgag cgcctggccc gtacgacttc ctgagtcaga gcccgtttac
     2641 cgggttccat tcctcatttc cccaccggac ccgacagcag ctccaccgcc ttcagcaacc
     2701 gcagcgtgtt tcgctggacg tgcgcgccga gtgccagcag cccgaacgcc gtgatggtgt
     2761 agatgctcac cgtcgcgatc agcagcagga catgcgttcg gttggaaaag tcggcgagca
     2821 gcaggaagcc gaccagaaag agcatctcga tcagcaccgc cacgatgaac gccatacggt
     2881 aattccgctc cgcctgctcc atgcgatcga gcgcggtgcg gcgggctcct tcgaattcgg
     2941 tggtcatcga atcctcctct tgtcatgcgg ttgaccgtcc tggatggccc tcaatgattc
     3001 cttatcagga tgtcgatcac gagtggtatc agcaccagcg cctcgacgat cacgaacgcg
     3061 atgatcgcct ttcggagtcc gtcatcgcgc ttcatgcggc cccagcgttt gtcgcagtgc
     3121 ggcgagtccg tacgccagtc gcgacttgac ggtgcccggc gcgatctcga ggacgtcggc
     3181 gatttcggtc aacgtcatcc cctcatggta gtgaaggacc agcaccgccc ggctggcggg
     3241 ggagaggccg gccatcaacc cgccgaaccg gcccggaacc atcgtcgcgg gagtccctga
     3301 ctcgtccgga acggcatcga gcacctccgg atcgcgcacc tgctcggacc agcgcctttc
     3361 ccgcttcagc ctcttgaagg cctcacgcga ggcgatccgg aacacccagg ggcggaagag
     3421 cgccgggtcc tgaagccacc gcagctttcg atagacgatg gtcaggacat cctgcaggag
     3481 gtcttccgcc ggtgcccggt tgccgaccaa ccccgagaga taacgaccga gcggcccttc
     3541 gaccccccgt agcagccggt caaccgcctc cctgtctccc gactgggcgc ggagcaccca
     3601 gaacgcctcc tggcggccgt tgtccgtcaa tcgtcctcca caccgggagt gaggcggcga
     3661 gtcggcgaac ggttcggtgg aaaatgcggt cggccgctta cggccgccgc cctaccagat
     3721 gctcacccgc acgtcgtccg cccgatgcat ccggtccccc ggcttcacgt tccaggcctc
     3781 gtagaactgc ggcaggttgg ccatcgggcc gttcacgcgc aggaaggcgg gggagtggac
     3841 gtcggtcttg atgcggagcg cctgcgtctc ggggcggaag tggttcatcc agccgagcga
     3901 ccagccgatg aagaagcgct gggccggcgt gaagccgccg agcttctttc cttccttgta
     3961 ctgcgccgtc tgcgtgaagg cgtcccatgc cagcagcatc ccccccaagt cggcgatgtt
     4021 ctcgccggag gtggccttgc cgttcacgtg caggctgtcg agcaccacgt agtcgttgaa
     4081 ctgggccacg atccgctcgg cgcgcttctc gaactcggcg gcgtcttcct tcgtccacca
     4141 gtcctcgagg tttcccttct cgtcgaactg gcggccgtcg tcgtcgaaac cgtgcgtgat
     4201 ctcgtggccg atggtcgtgc cgccggcgta gccgtacacg agggcgtcat ccacttcctg
     4261 gtcgtcgatg ccgggaagga tgaaggcggc cgccggcatc acgatctcgt tgttcgaggg
     4321 gttgtagtac gcgttgtacg tctgcggcgt catgtcccac tcggtgcggt ccaccggctt
     4381 gtggagcttg gcgatggcgt actccgaggc ccagatgttg ccgtacatga cgttcttgag
     4441 gtgcgattcg cggctcacgg gataattgga atagtcgcgc cacttgtcgg gatagccgac
     4501 cttcacggtc acgctcgaca gtttcttcag cgcccgctcc ctggtggcct cgctcatcca
     4561 ggtaaggttg tggatgcggg cgcggaaggc gtcgaacacc ttgttcgtca actcggcgta
     4621 gcgcagcttc gtctcggggc cgaagtagcg ctccacgtac agctcgccga gcgcgtcgcc
     4681 caggtagttc tcctcggcgt ccagcacgcg cttccagcgc gggcgctgcg tcttcgtgcc
     4741 ggccagcacc gtctcgtaga actggaagtt ctgccggtcg aacgcgccgc ccgccgcgtc
     4801 ggcaaacgtg ttggccaggt tccagcgcag gtaggtcttc cattcgtcga tcgaatgggt
     4861 cctgagcgac ttctcgacct ggcggaagaa ctcgggctgt cccaccacca ccttgtcgat
     4921 gtcccggatg ttcccggcct cgagcatggc cttccagtcg atggagggcg tgagcctggc
     4981 cagggtggcc accgtcatcg ggttgtagtt ggcctcggga tcgcgcaggt cctcgagctt
     5041 gcggctggcc ttcgccaggt cggtctcgag gcgcatgatc gtttccgacg ccttcttcgc
     5101 cgcttcgggc gtgtcgccca tcagctggag catcttggcc acgtgcgcca cgtattccct
     5161 gcggatgtgg gtcgagcgtg cgtccgggtc cacgtagtag tcgcggttgg gcaggccgag
     5221 gcccccctgg tagaggtgga gcacttcgtg ctccgaatcc ttctcgtcct ggaagatgta
     5281 ctgcccgtaa agcggcccgg cgccgatgta ctgcagccgg gcgatgacat ccagcaggct
     5341 cttccgatcg gtgatcgcat cgatccggtc gaactcggcc tggagcggct tgtaaccggc
     5401 cgagtcgatg gcggccgtat ccatccccgc cgcccagaaa tccccgatct tctgcgcgtt
     5461 gttgccgggc gccgcatccg tccggaccgc cgcctcctgg ttgatctcga ggaggcggtg
     5521 atacgtctcc tcctgcacca ccttgccgat tccccacgag cgctcgctct ccgggatcgg
     5581 gtgccgtttc agccagcccc cgaccgcaaa gtggaaaaag tcgtcgctcg gcgtcacggt
     5641 cgtgtcgacg aactgggcca ggtaggtgtc cgcggtgacc ttcggcgccg cctgggcggc
     5701 cggcttgtcg gccggcttcg gctggctgca gccggaagcc aggagggcga gcacggggca
     5761 cgcgaggagg gtaacgatcg atttcatcgg ggcttccatg ggagagagtg gcggattccg
     5821 gggacgcgga cggggcattc tagccgttcc cgggaggacg acaatcggtg tggacgtccg
     5881 aaaggtcgag cctgtgtgta aattcggcta tacactggtg atgcgagtca ctcctgtcgc
     5941 ggccgccgtg gagcggacgg cccgaaaccg acgaacatcg gaggtgccca ccatgaagcg
     6001 tcgtgagtac ggactcttcc tcctggccgc cgtgattctc ctgttgccgg gcatggccca
     6061 tgcggcgtcg atccacgacg cccgcttctt cttccacctg gcggccccgg tcggcaagaa
     6121 cccctgcagc cttcgcagca acccgccggc ctgcaatgac gtagtcacca aggggcaggt
     6181 ggggccgggc tacttcgcgt acatcgtcat cagcaacacc gatgccacgg gcgggctcaa
     6241 ctccggcaag ttcggcatcg actacaacgg caccgccggc gccggtgtgg acatcttcag
     6301 ctggacgaac tgctccagta tcgattttcc gtcgcccgga tggccgaatg ccgacaccgg
     6361 gaccatcctc acctgggacg ccgccagctg ccaccatgcc gtggcgtccg gcaccgtgat
     6421 ggccatcggc ggctatttct acctgagcgc ctacacgccc gacgagatcg agatgacacc
     6481 ccgccccctg gacaaccgcc tcgaggtgtc ggagtgcaac ggcgcggtcg tcaacctgga
     6541 tcccaccctg cagaacctcg gctgggcccg cttcaccgac accggcgccg cggagggtca
     6601 cgccccctgc aacttccgga acagcccgtg ccgcgttcgc ggtccgcaaa ccgtggcggc
     6661 cggccagggc ggcatcaact tcaccgtgga ccccgagtac gtcgcgccgc tcgggcactg
     6721 gatcatccag gggaacggcc agatctcgag cagcaacaat gcctcggcgg tcgtccaggc
     6781 gctgggcgcc ggcagcttca ccctcacata ccgcatcagt accggctgcg cggaagggtg
     6841 ccagtgcgac tacccggtaa atgtcgtcgg gaccgcgggg gtgccggccg cgccacccga
     6901 cacctcgccg acgacgtgga gccgggtcaa gggggcttac cgctagggcc ttcccgaaaa
     6961 gttgataccc gacgtcggaa acggcgctta ttgtcgtatt ccggcgcatg acgtcccgtc
     7021 gggtgggcca ttcgcctgcc cgacggtgac acatcggtat ccggggccga aggccccgtc
     7081 cacatgacag ggaggtcccc atgacgctcg cccgttccgt tcgtcgattc gtccccgttt
     7141 tgctggcgcc tgtcatcctg ttggcgcccg tgccgcccgc cctggccgcc ggcgagatca
     7201 ccgtggctaa ggacacgatt cggccgaaga tggatgcccg cggctgcatg atggatgaca
     7261 aggggtgcaa ggggggcgac ctcattgcct acccggggct catcgccgaa ttgaacgggg
     7321 cgctgccgag cggggcgcag ccgtacgtgg aattccggat gaacggcaag tcgatcaaga
     7381 tcgatgccgg cagcgacgac gtgtacggca tcgataaccg gacgaagatc ttcgtcaccg
     7441 acaatcacgg atcggagaac ggctgggtat ttcccggaaa gatcccggcg gggacggtgg
     7501 agttcaccat cggcgtccgg aacgaactca ccgagtcgaa cgacgtcatc taccagggca
     7561 agttcgacta cgaaaagcgc ctcatcagcg ccggcaacga ggagacggtg gaattgctgg
     7621 tcaacgacga ctggcgcctg ccgatcggct acctctacat gacgaaggac cgcggcctgc
     7681 acgtcgtcac ctggtatcgc ggtcgcccgg gcggggtaaa gacgtttctc ttccgtgacg
     7741 gcgtggaagt ggccaagaac gaagggtgcg gcatcggcga cgtggccgat ttcgacccca
     7801 cccattacat gtactgggaa gtggactgcg aaatcaccgg cgtgtacggc gacgaggaat
     7861 cggcgaagaa cggctacgag ccgaacatca acctggccgc caaccccggc aactacgagt
     7921 tcaagtgcct ggccggcggc aagctggccc gcgtgatcaa gttcaccgtc aacgcggacg
     7981 gctcgttcga caacggcatc gccaccgcca accagctcgg ctcggagcgc gtcatcgttc
     8041 cggtcgaggt caagatggac aaccccacct ggaacaaggc ggcctggaag acgggggcgt
     8101 actacggtca tccgctgacc ggcttcgcgg tgccggggca gccctgaggc caacgcgtac
     8161 gtcccgccgg gcgggagcgt cgacgaccgc atcggcctgt ggaccggtgt tctgctgcat
     8221 cctcctcgcc gtcgcgctcc tgttcggcgc ccgagtggcc ggtgcccgcc cctggtacaa
     8281 cgccaacttc gacggctatt ccctggcatc gggaagccgc atcttcccct acaatgccaa
     8341 taacgacgcc gagctcgaca tcatcacgag cagcggaaac gtgatcctgg gaatgccgaa
     8401 cggcgatttc tcccagggag tcgggttcac tattccgctc agcacatatc atatggcgat
     8461 cgctgatcag aacggtgatg gccgcaagga tgtcttcctg acagggaagc ccaccccggg
     8521 cgcctggggc acgttctatc tgtacaccta tcagaagctc gccggaaatg gattcacggc
     8581 gctgccgggc atcgtgctcc cggagaagtc cgacgtgctt cacgccggcg acttcgacgg
     8641 aaacggactg gccgacgcgg tggtgttcac gcgcggcctc gactttcccg atgtacccga
     8701 taccggcgcg gtctacttct cgcacccggg cgggatttct ccgggcgtgg tattcgaaat
     8761 cccgatgctc tatccgatcg actcgggagt cggcgatttc gacgaggacg gccggaccga
     8821 catcgtggtc ctctccgctt tcaacctgaa cccacggacg cttgcgtttt acctgagctc
     8881 ggcgggtggg atcctggccg ctccccggat cgacaccttg tcgacgatct tcccggggta
     8941 tatcggtgtg gccgatgtca acgacgatgg acatgacgac atcgccttca gcgacgggcg
     9001 cgcgccggct tcgtttctcg gcaatggcga tggaacgttc cagccggtgt acccgccgga
     9061 cgtcggtccg gcctggtgcg gcgatctgga gttcggcgat gtcaacctgg acggtcacct
     9121 ggacgtggtc agcaactgca cgttcaacta tgcctacatc ggcctgggag acggagccgg
     9181 tcgcttcagc acggtttcga ggcactggtt tccgcgagga gggagcgagg agtcgctgat
     9241 cgtcaacgac ttcgatcacg acggattcgg cgacatcgtg ggaaaccggt ctcccgggct
     9301 ggcggtgttg cgggggaacg cggccgtcac tttcaatacc gttccggagt ttccggccgc
     9361 ccccaacctc tccgaggtca tcgcgctcga ctgggatctc gacggcatcg aggatctcgt
     9421 gggcgtcgac cggatcgggg gcaacctggt tctcttccgg aaccggctcg gcgcgctgga
     9481 gcccggcgtc acgattcccc tgggtttcat tcccgacggc atccggttcg cggacctcaa
     9541 cggtgatgga cgccgcgaca tcgtggccgc cgaccagggt gccgacctgg tcaaggtgtc
     9601 gctcgggagc agcgggggtg gcttccagac gccggtcagc tatcccgtcg gaagcgaccc
     9661 gagcgacgtc gcgacgggcg acttcaatgc ggacggcata ctcgacgtcg tcgtaagcaa
     9721 cttcagctat cccagctggt cggccagcta cctccggggg acgggcaacg gtgctttcgc
     9781 tccgcaagtc gtcattccaa ccgctgcgac accgagctcg atcgaaacag gcgacttcaa
     9841 cgaggacggc aaactcgact tcgtgaccgg tcatcccttc gacaggtgca gcctggtcct
     9901 tggacgcggg aatggaacgt tcgacccgta tattggcgtc ggaacg
//
LOCUS       DASVOD010000407         7439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_289031, whole genome shotgun sequence.
ACCESSION   DASVOD010000407 DASVOD010000000
VERSION     DASVOD010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7439
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289031"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VF720_09340"
     CDS             <1..817
                     /locus_tag="VF720_09340"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7879600.1"
                     /translation="VSTLSGKQVGSVSRIGKRLVWHLEDDLHVVIHPMVAGRFHLRKP
                     DAAVPRKNTLAVFDLPADDTRPAARLLLTEASTKKRATMSLVRGQEALEAFRAGGLDV
                     MTATDAEFSAALRRENHTIKRSLTDPRLFDGIGNAYSDEILHRARMSPMKLTRRLTDP
                     EIARLAAATRETLSLWIHRHRQKAGDKFPEKVTAFHDDMAVHGKFGKPCPDCGTPVQR
                     IVYAENECNYCAVCQNDGRLLADRALSRLLRADWPRTLEDLEALRRGAGTGPK"
     gene            824..1171
                     /locus_tag="VF720_09345"
     CDS             824..1171
                     /locus_tag="VF720_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015919331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX7879601.1"
                     /translation="MSRLKQFREYREKMNQKILEAGNLEINRFFALDTRCYEAGALPV
                     KTKELMGLTASMVLRCDDCIAYHVIRAVEEGCTDAEIREAMSVSLVVGGSIVIPHLRR
                     AFELLEEVRGAGN"
     gene            complement(2705..3601)
                     /locus_tag="VF720_09350"
     CDS             complement(2705..3601)
                     /locus_tag="VF720_09350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879602.1"
                     /translation="MSGAQTPRAAGRRRGTAATRLPDTAVDPAYSRLAPHYDRVFGSY
                     ARELNRWLVPWVKRLDPRPRSALDLACGTGTTALALARVMPRCLAIDLRPEFAAIVSA
                     RAGKAASTRHLEARTGDMRTFRLAQRVDLVTCFFDAINHLPRPTDIDRTFKSVHRALA
                     PGGLFLFDINTPWALKKTWPEMKAIWKGRGWFAVARGRYVKAREPRGPENNGSKRGHP
                     RGARGHGEFDIHWFMRDTDGAYAPRVERFREIAWTAAEVRAALKKAGFAEIRTLNEPG
                     LAPFVGETGKDRTFWVARREDA"
     gene            3640..4767
                     /locus_tag="VF720_09355"
     CDS             3640..4767
                     /locus_tag="VF720_09355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7879603.1"
                     /translation="MKFVLVGTAYPHRGGIAHFVHLLAHTLTARGHDVSILSFCRQYP
                     SILFPGTSQDDDGPPPDPPLDIHPLIDTINPLSWARAARWAAAQKPDLTVFKYWMPFF
                     APGYDAIVRHLDRKQVGRTCFLLDNVIPHEPRPGDRWLTKRVLGKVDTFIAQSAVVRD
                     DLLKFRPDAVYRDVPHPVYSSFPQGRSREDARARLGVKQRNVILFFGFIRAYKGLMNL
                     IRTMPHVASDVGLLIGGEFYEDDKPYKAEVARLGLTDRVTWHDRYIPNEEVGDFFNAA
                     DAVVLPYQSATQSGIIQIAMNYGTPIITTNVGGLAEVVKDGVMGYVVPPEDPEALAAA
                     INRFFRDERREAFSTAVTAERERYSWTPMAEALEAFARGET"
     gene            4764..6092
                     /locus_tag="VF720_09360"
     CDS             4764..6092
                     /locus_tag="VF720_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7879604.1"
                     /translation="MKKVLIVAYYFPPSGGPGVQRVLKFAKFLPEFGWEPAILTVMDG
                     DYPARDESLLDEIPAGLPVHRTPILEPYSIYRSLTGKKKGAAVDVNTNYRPGTKVPLS
                     EKVAQGIRGALFIPDARVGWLVHGLPAGVRIARNWGADLIFSSSPPYTCALLGRGIAR
                     RAKRPWVFELRDPWTGFLSAPVRHGPSKAIDRHLEKSCVRDASRVVVAWKGIQRDLVG
                     KYPDLKTDHFRLIENGFDPDDLSGVEPVHFDRFTVVYTGSMYGVRNPDTYLRAVARLL
                     ETGRIKATEFKTVFVGRFGDEVRAMFHRPELQGTVEEVGHRPHSESVRYTLGAQALLL
                     VVDDVAGSEGIVPGKVFEYLGARRPLLAVAPEGDIADLVRRTEGGVVVDRNDIDRMAN
                     TLAGWVDEYRKTGAVLFRGRDEEIDRLSRRGRTGLLAQTFDEVTAGQAAK"
     gene            6140..>7439
                     /locus_tag="VF720_09365"
     CDS             6140..>7439
                     /locus_tag="VF720_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879605.1"
                     /translation="MNKPRETAKARTKANEPRTTRATVEAPPPGIFGQYPTATGLVVL
                     ALTLLVFFAPMVFSGKVFLPPDNIASLSHQPYLAEVKAQGEYPLWTPYLFSGMPSLGS
                     LIAAPGTNPFSTVLAPLGPALKPVAWYFLIGLFTFLLARRRVESRFAALFAAVAFVYC
                     AHVIGWVMAGHNSKLATTVFFPAILLLVDRLVARPSPLNWALMALAIGLSIVTSHMQI
                     TYYALMSAGLLLLFLSVDRLKGGGKPLTVAIPWVLFGLAVLIGLGASAIISLPVQEYS
                     HWSIRGAEGAGLTRQYATNWSFHPMEMLTFFVPAYFGFGNPTYWGWMPFTEAPHYMGI
                     LPLFLAVLGAALRWKDALTKFLVALAVLGLFIAFGKELPLVYDLFFNFMPMFNKFRVP
                     SMALILTQLSVAVLAALAIDTLARSATAAEREARAATFRKT"
BASE COUNT         1254 a         2478 c         2435 g         1272 t
ORIGIN      
        1 ggtgtcgacc ctgagtggca aacaggtcgg gagtgtttcg cgaataggca agcggcttgt
       61 ctggcatctc gaggacgatc tgcacgtcgt catccatccc atggttgccg gacgtttcca
      121 cctgcgaaag ccggatgccg ccgttccgcg gaaaaacacc ctggccgtat tcgacctgcc
      181 ggccgacgac acgcgtccgg ccgcgcgcct tctcctcacc gaagccagca cgaagaaacg
      241 agccaccatg agcctcgtcc gcggccagga ggcgctcgag gcctttcgcg ccggtggcct
      301 cgacgtcatg accgccaccg acgccgagtt ctccgccgcc ctgcgccgcg agaaccacac
      361 gatcaagcgt tcgctgaccg acccgcgcct cttcgacggc atcggcaacg cctactcgga
      421 cgaaatcctc caccgcgcgc ggatgtcccc catgaaactg acccgccggc tgaccgatcc
      481 ggagatcgcc cgcctcgccg ccgcgacacg cgaaacgctg tcactctgga tccatcgcca
      541 ccggcagaag gccggcgaca agtttcccga gaaggtgacg gccttccacg acgacatggc
      601 cgtgcacggg aaattcggca agccctgtcc cgattgcgga acgccggtgc agcggatcgt
      661 gtacgccgag aacgaatgca actactgcgc cgtatgccag aatgatggcc gcctgctggc
      721 cgatcgggcg ttgtcgcgtc tcctccgggc cgactggcca aggacgctgg aagatctcga
      781 ggcgctccgg aggggcgccg gcaccggacc caagtgagga gccatgagtc gcctgaagca
      841 gttccgcgaa taccgcgaga agatgaacca gaagatcctc gaggccggca acctcgagat
      901 caaccgcttc ttcgcgctcg acacccgctg ctacgaagcc ggtgccctgc cggtcaagac
      961 caaggaactg atgggcctca ccgccagcat ggtcctccgc tgcgacgact gcatcgccta
     1021 ccacgtcatc cgagcggtcg aggaaggatg caccgacgcc gagatccgcg aggcaatgtc
     1081 ggtgtcactg gtggtgggcg gctcgatcgt gattccgcat ttgcggcggg ctttcgagtt
     1141 gctggaggag gtgcgggggg cggggaactg aaccggtcac ttgtgtcagt tgggggacgg
     1201 aggcgcgaaa aaatcggtcg cctgtagcgt accgaaccag ttcctttggt tgagcggttc
     1261 gaacaggttc cggaacctgt tgatgatccg cggcgggccc gatcgctcac atgatccggg
     1321 cgctttcgcg ccttgcgtcg aatgttctcg tcgccgaact cgagccggcc ctcatgctga
     1381 ttgtgggcgc gacaccgcaa tgtgatgttg tcgaggctcg ccgggccccc gagagcaaat
     1441 gggatcacgt gatggaactc caagtgaccc atcgccgtgc agcgccggcc gtgcgacgcg
     1501 cggaaggtgc accgatttcc atcccgccgg accacctctc gtcgcacgcc tgccggtatc
     1561 cggcgggacg agccggacgc cgacgggatc gcgcgcgaac gacggggttt ccgaacctcg
     1621 gcgaaccggc gtcgcttgag gtcttccacc aggcggggta aggccagctt caggatggcg
     1681 gacgtgtcac ccccgggaat ctggtgtcgc aataattcct gagcgacccg gaagtcggcg
     1741 taggtctcct cgtccgcgct gaactggaat cggtatcgac cgggcgaggt ggggcgggga
     1801 gggccaagct gggcaggagc attggccgct ggcattgttg tcgcagagtg gggaggcggg
     1861 gccgctgctt ggacccggcc aaccaccgac ggatccagcc tgtcgaccac cgaaggatgc
     1921 accccgtcga ccaccgccga atccaccccg tcgacccccg acggatccat cccgtcgacc
     1981 cccgacggat ccatcccgtc gactccggac gcgccgatcc gatcgaattc cgggacaacc
     2041 aggcagttgg ccgtgtcatt gccaaacaag tcgggcaccg gcctggatcc ctgccgggtc
     2101 ggcgccgatt cgaatcccgt tgcgatcggc gccgccatcg gcgaccgccg tacgctggca
     2161 atacagtccg gctgcgggct caactcgcaa acgagctgct cgatctcgcg ctttcccagg
     2221 tgtcgcgcgc gcgccaacac ggcacggtga ttatcgcggg tcaggaccgg cgccaggagt
     2281 cgcactgccg tgacatgcag tgatccatcc tcgaggagct tcaggatgtg cggcaggcga
     2341 cgtgccgccc gtgcgcactt gattcgtgcg tatgccgcgt cctccgagaa gtggagcacc
     2401 tccgtgcacc aggtgaacat cgaggagcag ccttccgcca ggtacagttt tcgtctgtcg
     2461 acttcgccga ggtaggcaat caattccgca gtgagacggt ggtcgcgatg gaccagcgcc
     2521 gtcaggcggc tgagcagttc cgcgcgggag agtcgggcca atgaggcaag tggagtcatc
     2581 atgacacttg catagcatgt cgtgatgtcg aactgtcaat ggagaagtgt ccgcaagtgg
     2641 cccgaatcgc gatcagaaac tgtaatgcaa tcagggtccg atcgaggcgg atttcgaagg
     2701 ctcgtcaggc gtcctcgcgc cgcgccaccc agaacgtacg atcctttccc gtctccccga
     2761 caaacggcgc gagacccggc tcgttcaacg tacggatctc ggcaaagccc gccttcttca
     2821 gcgcagctcg cacctcagcg gcggtccagg cgatctcgcg aaaccgttcg acgcggggtg
     2881 cgtacgcgcc gtcggtgtcg cgcatgaacc agtgaatgtc gaattcgccg tgaccccgcg
     2941 ccccccgtgg atgtccgcgc ttcgaaccat tgttttccgg accgcgaggt tccctggcct
     3001 tgacatagcg cccgcgcgcc accgcgaacc agccgcgtcc cttccagatg gctttcattt
     3061 cgggccacgt tttcttgagc gcccacggtg tattgatgtc gaaaagaaag aggccaccgg
     3121 gcgcgagcgc ccggtgaacc gatttgaagg tgcgatcgat gtcggtcggg cgggggagat
     3181 ggttgatcgc atcgaagaag caggtgacca ggtcgacgcg ctgggcgagt cggaaggtcc
     3241 gcatgtcgcc cgtccgggcc tcgaggtggc gggtcgaggc ggccttgccg gcgcgggcgg
     3301 acacgatggc ggcaaattcg gggcggaggt cgatggccag gcagcgcggc atgacgcgcg
     3361 ccagggccag cgcggtggtg ccggtgccgc acgccaggtc gagggccgag cgggggcgtg
     3421 ggtcgagcct cttcacccac gggacgagcc agcggttcag ctcccgggcg tacgaaccga
     3481 agacgcggtc gtagtggggg gccagtcgcg agtaggcggg atcgacggcg gtgtcgggca
     3541 ggcgggtggc cgcggtacca cgacgccggc cggccgcgcg cggcgtttgg gccccgctca
     3601 tcgcttgcgg cacccaacgg ggcgggctac tgtccctgcg tgaaattcgt cctggtcggc
     3661 acggcgtatc cgcatcgcgg cggcatcgcg cattttgtcc acctcctggc gcacacgctg
     3721 acggcccgcg gacatgacgt cagcatcctc tccttctgcc ggcagtaccc gtcgatcctt
     3781 ttcccgggca cctcgcagga tgatgacgga ccgccgcccg atccgccact ggacattcac
     3841 cccctgatcg acacgatcaa cccgctgtcc tgggccaggg ccgcccgctg ggccgccgcg
     3901 cagaagccgg acctcacggt cttcaagtac tggatgccgt tctttgcccc cggatacgac
     3961 gccatcgtcc ggcacctgga tcggaaacag gtcgggcgga cctgtttcct gctcgataac
     4021 gtgatcccgc acgagccccg cccgggcgat cgctggctga cgaaacgggt gctggggaag
     4081 gtggatacgt tcatcgccca gtcggcggtg gtgcgggacg acctgctgaa gttccggccg
     4141 gatgccgtct accgcgatgt gccgcatccc gtgtactcga gcttcccgca gggaaggtcg
     4201 agggaagacg cccgggcccg gctcggcgtg aagcaacgca acgtgatcct ctttttcggg
     4261 ttcatccgtg cgtacaaagg gctgatgaat ctcatccgca cgatgcccca cgtcgcgagc
     4321 gacgtggggc tgcttatcgg cggcgaattc tacgaagacg acaagcccta caaagccgaa
     4381 gtcgcccgcc tgggcctcac cgaccgcgtc acctggcacg accgctacat tcccaacgag
     4441 gaagtgggcg acttcttcaa cgcagccgac gccgtcgtcc ttccttacca gtccgccacg
     4501 cagagcggca tcatccagat cgcgatgaac tacggaacgc ccatcatcac gaccaacgtg
     4561 ggcggactgg ccgaggtcgt gaaggatggg gtgatgggct acgtcgttcc gcccgaagat
     4621 cccgaggcgc tcgcggccgc gatcaaccgc ttcttccgcg acgagcggcg cgaggcgttc
     4681 agcaccgccg tgaccgccga acgcgagcgc tattcctgga cgcccatggc cgaggcgctg
     4741 gaagccttcg cgcgggggga gacgtgaaaa aggtccttat cgtcgcctac tacttccccc
     4801 ccagcggggg gccgggcgtt cagcgcgtcc tcaagtttgc caagttcctg ccggagttcg
     4861 ggtgggagcc ggcgatcctg accgtaatgg atggcgacta cccggcgcgc gacgagtcgc
     4921 tgctcgacga gatacccgcc gggctgccgg tccaccggac gccgatcctc gagccctatt
     4981 cgatatacag gtccctcacc ggcaagaaga agggcgccgc cgtggacgtg aacacgaatt
     5041 accgtcccgg cacgaaggtg ccgctctcgg agaaggtggc ccaggggatc cggggcgccc
     5101 ttttcatccc cgacgcccgg gtggggtggc ttgtccacgg ccttccggcc ggggttcgga
     5161 tcgcccgtaa ctggggggcg gacttgatct tctcctcgtc gcccccctac acttgcgccc
     5221 ttctcggacg ggggatcgct cgacgcgcca aacgaccgtg ggtcttcgag ttgcgggatc
     5281 cgtggacagg gtttctgtcg gcgccggtgc ggcacgggcc gtccaaggcc atcgaccgtc
     5341 atctcgagaa gtcgtgcgtc cgggacgcct cccgggtggt cgtggcctgg aaggggattc
     5401 agcgcgacct ggtcggcaag taccctgact tgaaaacaga ccacttccgg ctcatcgaga
     5461 acggtttcga ccctgatgac ctctcaggtg tcgagccggt ccatttcgat cgcttcactg
     5521 ttgtctatac aggctccatg tacggcgtcc gcaacccgga cacgtacctg cgcgcggtgg
     5581 cgcgcctgct cgagacaggt cgcatcaagg ccactgagtt caaaacagtc ttcgtcggtc
     5641 gcttcggcga cgaagtccgg gccatgttcc accgcccgga actccagggc accgtggaag
     5701 aagtcgggca ccgaccgcac agcgaaagcg tgcggtacac ccttggcgcg caggcgctgc
     5761 tcctcgtggt cgacgacgtg gccggcagcg aggggatcgt tccgggaaag gtgttcgagt
     5821 atctcggcgc gcgccggccg ctcctggccg tggcgccgga aggggatatc gcggacctcg
     5881 tgcgacgcac cgaagggggc gtcgtggtcg accggaacga catcgacagg atggcaaata
     5941 cgctggccgg ctgggtggac gagtaccgga agaccggcgc cgtcctcttc cgcggacgcg
     6001 acgaggaaat cgacaggctg tcacgccgtg gccggacggg cctcctggcg cagacattcg
     6061 acgaggtcac ggccggccag gccgcgaaat agcggccgac atggccgcga cgaggtaatg
     6121 ggatgatggg agtcgatcga tgaacaagcc gcgcgagacc gcgaaggcgc gcacgaaggc
     6181 caatgaaccg cgcaccacgc gcgccaccgt cgaggcgccg ccgcccggca tcttcggcca
     6241 gtatcccacc gccaccggcc tcgtggtcct ggcgctgaca ctcctcgtct tctttgcccc
     6301 gatggtcttc tcggggaaag tgttcctgcc gccggacaac atcgcctcgt tgagccatca
     6361 gccgtacctg gccgaggtga aggcccaggg tgagtaccct ctctggacgc cttatctctt
     6421 ctccggcatg ccgagcctcg gcagcctcat cgccgcgccg ggcaccaatc cgttcagcac
     6481 ggtgctggcg ccgctcgggc cggcgctcaa gccggtggcc tggtacttcc tgatcggcct
     6541 cttcacgttc ctcctggcgc gacggcgggt ggagtcgcgc tttgccgcgc tctttgcggc
     6601 ggtggcgttc gtctactgcg cgcatgtcat cggatgggtg atggccggcc acaattccaa
     6661 gctggcgacg accgtcttct tcccggccat cctgcttctt gtcgatcgcc tcgtcgccag
     6721 gccgagtccg ctcaactggg cactcatggc actggccatc gggctctcga tcgtcacgtc
     6781 gcacatgcag atcacctatt acgccctgat gtcggcgggc ctcctcctcc tgttcctgtc
     6841 ggtcgaccgg ctgaaaggtg gcgggaagcc gctcaccgtc gccatcccgt gggtgctgtt
     6901 cgggctggcc gtgctcatcg gactcggcgc gtcggccatc atcagcctgc cggtgcagga
     6961 gtacagccac tggtcgatcc gcggcgcgga aggggcgggg ctcacccgcc agtacgccac
     7021 gaactggtcg ttccatccga tggagatgct caccttcttc gtgccggcct acttcggctt
     7081 cggcaacccc acctactggg gttggatgcc gttcaccgag gcgccgcact acatggggat
     7141 cctgccgctc ttcctggcgg tgctgggggc cgcgcttcgc tggaaggacg cgctcacgaa
     7201 attcctggtg gcgctggccg tgctcggcct cttcatcgcc ttcggcaagg aactcccgct
     7261 cgtctacgac ctcttcttca acttcatgcc gatgttcaac aagttccgcg tgccgagcat
     7321 ggcgctgatc ctcacgcagc tgtcggtggc ggtgctggcg gcgctggcca tcgacacgct
     7381 ggcacgcagt gcgacggccg ccgagcgaga ggcgcgcgcg gccacgttcc ggaagacgc
//
LOCUS       DASVOD010000408         4921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_29608, whole genome shotgun sequence.
ACCESSION   DASVOD010000408 DASVOD010000000
VERSION     DASVOD010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29608"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..3526)
                     /locus_tag="VF720_09370"
     CDS             complement(29..3526)
                     /locus_tag="VF720_09370"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEX7879606.1"
                     /translation="MKTFNLYLRNYFEPGDAQKLAKFDAVALDVDAPTSVLNDIHSYN
                     PNCRILAYIPINGTYDNANIFPAGSKWRAVWEAAEANNWWLRDTQGGLIYDHPGKRTT
                     NCTFNCPVNGQGQSYMDWFPQFIAGTVLQNGAAPWDGVLLDDVWIGIWFVGNNRTLNA
                     LPIDADRDGTGDDQPTLDGWWKAGNDSLTARIRRLMPANEIVIGNGGNNFEQINGSYI
                     ESFPFNGLVDQNHPLGYSWTDKMWGSYGYFPNEALYSNQPLRMNVIDAKWSQGDRYNP
                     VVSPAFERHKRFCMASALMSDGYFSLDRHASDGAHDSIWWFPEYDKPIGTPTGPATQV
                     SYNGVTLWRRNFTNGVVLLNNNYATFTGSTASSIPPIGWVDARVLLNSELWHAEGVPP
                     AAITDLAITRTWNNSAEVGWTNVGDDGTTGQAIQVEVRYSTSPITAANWGQASVASSS
                     PIPELPGLTQTHIVTNLLSGQTYYFAAKTKDLSGNWGAISNVVSGLTVPGDVVGPAAI
                     TNLSVIATTATTATIQWTATGDDGSIGTASSYDIRYLPNLLSETTWGQGSQVVGEPVP
                     TPAGSVQQLTLYGLSPSAVYAVGIKVSDEMPNTSPLSNVAVISTQAGDTTPPAAIQDL
                     RFTGLGTNTVTLTWTAPGNDGIVGRANQYDMRWSRNPITAANFEFAGFITTEPGPALA
                     GSTQSLTLNMGTGYTYYFAMKTTDDWGNVSGMSNVSSGTTNTTDTGAPGAITTLAVTA
                     TGPTSVALAWTATGDDGQTGTASSYEMRWSNSPITPANFTGATPVAWMPLPAAPGTLQ
                     TKTVGNLATGQPHYFAIRIADERSTWSPISNVVTTTTGAGSDLTAPAAIADFRVLSFT
                     TNSVTVAWTAPGDDGATGTASSYDMRWSTTSISEINFATRSYITTEPQPAVAGTTQQL
                     TIVGFSPGRTYYLALKTADEKPNWSAMSNLLSFTTLIDNTPPATISDLVGMLLAGDTV
                     ELGWTAPGDDNMTGLATTYDVRMATAPITDANFDQSPSLVGEPPPANPGSAQGFEVSD
                     LLPGLTYWVAMKAIDDVGNRSAVSNVLTVETPATSGVPGPIAAPGLYLRSPNPVRTAA
                     RLELVLPAGTTGALSVSVFDVTGRVRRHLVDGPATPGAASLDWDGLDDSGRELANGVY
                     FVGAAAPGFERVVRIVMTR"
     gene            3906..>4921
                     /locus_tag="VF720_09375"
     CDS             3906..>4921
                     /locus_tag="VF720_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX7879607.1"
                     /translation="MKDSRIDKLARNLVRYSTQLKPGENVLIEAYDVPPEMVAALVRE
                     TRDVGANPMVLIKDNRVLREIYRLGDEGSMKLAGELERAWMDRMQAYIGIRGADNITE
                     LSDVPTAKMKLYQKLWWNPVHSEQRVNKTRWVVLRYPTSAMAQQANMSTDAFEDFYFD
                     VCANLDYTRMSEAMTPLQKRMMSTDKVRLVARDTDLTFSIKGIPAIKCDGHRNIPDGE
                     VYTSPVRDSVNGVISFNAPTIYQGVPFDNIRLEFKNGKIVKASGSNTERLNEFLDTDE
                     GARYIGEFAIGFNPYITKPMKDILFDEKIAGSIHFTPGQAYEIADNGNRSQIHWDMVL
                     IQTP"
BASE COUNT          875 a         1501 c         1680 g          865 t
ORIGIN      
        1 cgcgtccagt cgtgtgaggc ttgaatgctc agcgcgtcat gacgatgcgt accacgcgct
       61 cgaaaccggg cgccgcggcg ccgacgaaat agacgccgtt ggcaagttca cgtcccgagt
      121 catccaggcc gtcccagtcg agagatgccg ccccgggcgt ggccggacca tcgaccaggt
      181 ggcggcgcac gcgtccggtc acgtcgaaca ccgacaccga gagcgccccg gtcgtaccgg
      241 ccggaagcac cagttccagg cgtgcggcgg tccgcaccgg gttcggcgag cggagataga
      301 ggccgggcgc cgcgatcgga ccgggaacgc cggacgtggc aggggtttcc accgtcagca
      361 cgttgctgac ggccgatcgg ttgccgacgt cgtcgatcgc cttcatcgcc acccagtaag
      421 tgaggcccgg caggagatcg gatacctcga atccctgggc ggacccggga ttggcgggcg
      481 gtggctcgcc gacaagagat ggtgactggt cgaagttggc gtcggtgatc ggcgcggtgg
      541 ccatcctcac gtcgtaggtc gtggccaggc cggtcatgtt gtcatcgccg ggcgccgtcc
      601 agcccagctc gaccgtgtcg cccgccagga gcatgccgac caggtccgag atcgtggcgg
      661 ggggcgtgtt gtcgatcagc gtcgtgaacg aaaggagatt cgacatggcg gaccagttcg
      721 gtttctcatc cgccgtcttc agcgccaggt agtacgtgcg gccgggcgag aagccgacga
      781 tcgtgagctg ctgcgtcgtg ccggccacgg cgggctgcgg ctcggtggtg atgtaactgc
      841 gcgtggcgaa gttgatctcc gagatgctgg ttgtcgacca gcgcatgtcg tacgacgagg
      901 cggtgccggt ggccccgtcg tcgcccggag ccgtccaggc cacggtgacg ctgttggtcg
      961 tgaacgagag gacccggaaa tcggcgatgg ccgccggcgc cgtgaggtcc gaacccgcgc
     1021 ccgtcgtggt ggtgacgacg ttcgaaatcg gggaccaggt ggagcgctca tcggcaatcc
     1081 ggatcgcgaa ataatgaggc tggccggtgg ccaggtttcc gaccgtcttc gtctggaggg
     1141 tgccgggcgc cgccggcagc ggcatccacg ccacgggagt ggcgccggtg aaattggccg
     1201 gcgtgatggg cgagttcgac cagcgcatct cgtaactcga ggcggtgccc gtctggccgt
     1261 cgtcgccggt ggccgtccag gccagggcaa ccgacgtcgg gccggtggcg gtgacggcca
     1321 gtgtggtgat ggcgcctggc gcacccgtgt cggtggtgtt ggtggtgccg gaagacacgt
     1381 tcgacatccc cgatacgttc ccccagtcgt ccgtcgtctt catggcgaag tagtaggtgt
     1441 aacccgtccc catgttgagc gtgagcgatt gcgtggagcc ggccagcgcc ggtcccggtt
     1501 cggtcgtgat gaagccggcg aactcgaaat tggccgccgt gatcggattt ctcgaccagc
     1561 gcatgtcgta ctggttggcg cggccaacga tgccgtcgtt gccgggcgcc gtccaggtga
     1621 gcgtgaccgt attcgtgccg aggccggtga agcggaggtc ctggatggcg gcggggggtg
     1681 tggtgtcgcc ggcctgcgtg ctgatgacgg ccacgttgga gagcggcgag gtgttgggca
     1741 tctcgtccga caccttgatg ccgaccgcgt acacggctga cggcgacaaa ccgtacagcg
     1801 tgagttgctg caccgagccg gccggcgtcg gcaccggctc cccaaccacc tgcgatccct
     1861 ggccccaggt ggtttccgag aggaggttgg ggaggtagcg gatgtcgtag ctggaggcgg
     1921 tcccgatcga accgtcgtcg ccggtggccg tccactggat ggtggcggtc gtggccgtgg
     1981 tggcgataac cgagaggttc gtgatcgcgg cggggcccac cacgtcgccc gggaccgtga
     2041 gaccggacac gacgttcgag atggcgcccc agttgcccga caggtccttc gtcttcgcgg
     2101 cgaagtagta ggtctgtccg gagaggagat tggtgacgat gtgcgtctgc gtgagccccg
     2161 gcagctcggg aatcggcgag ctcgacgcga ccgaggcctg gccccagttg gccgccgtga
     2221 tcggcgaggt gctatagcgc acttccacct ggatggcctg gccggtcgtg ccgtcgtccc
     2281 ccacgttggt ccagccgact tccgccgagt tgttccaggt acgggtgatg gccaggtcgg
     2341 tgatggccgc cggcggcacc ccctccgcgt gccacagctc gctgttcagg agcacccgcg
     2401 catccaccca gccgatcggc gggatggaac ttgccgtgga gccggtgaag gtggcgtagt
     2461 tgttgttgag gagcacgacg ccgttggtga agttgcggcg ccagagcgtc acgccgttgt
     2521 aactcacctg cgtggcgggt ccggttggcg tgccgatcgg cttgtcgtat tccgggaacc
     2581 accagatcga gtcgtgggcc ccgtccgacg cgtggcgatc cagcgagaaa tagccgtccg
     2641 acatgagcgc cgaggccatg cagaagcgct tgtggcgctc gaaggcgggc gaaaccacgg
     2701 ggttgtaccg gtctccctgg ctccacttgg catcgatcac gttcatccgg agcggctgat
     2761 tgctgtagag cgcctcgttg ggaaaatagc cgtagctccc ccacatcttg tccgtccagg
     2821 agtaaccgag cgggtggttc tggtccacga ggccattgaa cgggaacgac tcgatgtacg
     2881 agccgttgat ctgctcgaaa ttgttgccgc cgttcccgat gacgatctcg ttggccggca
     2941 tcaggcgccg gatgcgggca gtgagcgaat cgttgccggc cttccaccaa ccgtccagcg
     3001 tgggctggtc gtcgccggtg ccgtcacgat cggcatcgat ggggagggcg ttcagggttc
     3061 ggttgttccc gacgaaccag atgccgatcc agacgtcgtc gaggaggacg ccgtcccagg
     3121 gagcggcgcc attctgcagg accgttccgg cgatgaactg cgggaaccag tccatgtaac
     3181 tctgtccctg gccgttcacc ggacagttga aggtacagtt cgtggtgcgc ttgccgggat
     3241 ggtcgtagat gagacctccc tgcgtatcgc gcagccacca gttgttggct tccgccgctt
     3301 cccacaccgc gcgccacttg ctgccggccg gaaagatgtt ggcgttgtcg tacgtgccgt
     3361 tgatcgggat gtacgccaga atcctgcaat tcgggttgta cgaatggatg tcgttcagca
     3421 ccgaggtggg ggcgtccaca tcgagcgcca cggcgtcgaa ctttgccagc ttctgcgcat
     3481 cgcccggttc aaaataattc cgaaggtaca gattgaaagt tttcacggcg ccgggagccg
     3541 ccgcggccgg ttgcgccccc gcgatcagaa tcagacatag ggaaaggaaa aagcgcgcgt
     3601 ggatccgcct catcaagagt gctcctcgtt ttacgatcct ggatggaaaa catcgcggcg
     3661 cgatcgggcc cggcgtcgcg gcacgccaag gcggaggcag gggattcggc gggtgtcagg
     3721 cgttggggga ttcccctatt gatcaggcat gatgccgatc gcgccgcaga aaagccagaa
     3781 aaaattcgga ttgactgacg gaaatttgaa cgccggacga tgctgactta cgattcgcgc
     3841 aacatcggga aaaagctgcc cgatcggccc acgccatcgg tgcgccatca agaaggagga
     3901 tattcgtgaa ggattcgaga atcgacaagc tggcgcggaa cctggttcgc tattccaccc
     3961 aattgaagcc cggcgagaat gtgctgatcg aagcgtacga cgtgccgccc gaaatggtgg
     4021 cggccctggt gagggagaca cgcgatgtcg gtgccaatcc gatggtactc atcaaggaca
     4081 atcgcgtgct ccgcgagata taccgcctcg gcgacgaggg ttccatgaaa ctggccggcg
     4141 agctcgagcg cgcgtggatg gaccggatgc aggcctacat cggcatccgc ggggcggaca
     4201 atattaccga gctgtccgat gtgccgaccg cgaagatgaa gctctaccag aagctctggt
     4261 ggaaccccgt gcacagcgag cagcgcgtga acaagacacg gtgggtggtg ctccgctacc
     4321 cgacctcggc gatggcgcag caggccaaca tgagcacgga tgcgttcgag gacttctatt
     4381 tcgacgtctg cgccaacctc gactacacgc gcatgtcgga ggccatgacg ccgctccaga
     4441 agcggatgat gagcaccgac aaggtccgcc tggtggccag ggacaccgat cttacctttt
     4501 ccatcaaggg gattcccgcc atcaagtgcg acggccatcg gaacatcccc gacggcgagg
     4561 tctacacgag ccccgtgcgc gactcggtga acggcgtcat ctcgttcaac gcccccacca
     4621 tctaccaggg cgtgccattc gacaacatcc ggctcgagtt caagaacggc aagatcgtga
     4681 aggcctccgg ctccaacacc gagcgcctga acgaattcct cgacaccgac gagggagccc
     4741 gctacatcgg cgagttcgcc atcggcttca acccctacat cacgaagccg atgaaggaca
     4801 tcctcttcga cgaaaagatc gccggctcca ttcacttcac ccccggccag gcctacgaaa
     4861 tcgccgacaa cggcaaccgc agccagatcc actgggacat ggtcctgatc cagacccccg
     4921 a
//
LOCUS       DASVOD010000409         5512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_600535, whole genome shotgun sequence.
ACCESSION   DASVOD010000409 DASVOD010000000
VERSION     DASVOD010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600535"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF720_09380"
     CDS             complement(<1..191)
                     /locus_tag="VF720_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879608.1"
                     /translation="MYKKFWPGVVLGAILTLAAMVGGRWLAPPSDAKSPAKPGDRGGL
                     VTSFGVGGVLTRDGELWQYR"
     gene            complement(214..906)
                     /locus_tag="VF720_09385"
     CDS             complement(214..906)
                     /locus_tag="VF720_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEX7879609.1"
                     /translation="MARDRAFPLALNESEPDPVITDTIILDIDTQWDLVMPDGAWPVP
                     GAFGLNPVFERLTRYARTYDIRVVATCQALASNDPRFGSADGMPPPYCVAGSPGARKI
                     PATRPGKPVVVPARPMGSGELKALIGDAREIVVETGGPDLMSNPGTAEILTWVRGAIV
                     FGVGGEEAVLNAVRALKSKGITVDVVQNAVALRSAEPEARDEWLETVRGLGARIIRDL
                     DVMTRHTTARHH"
     gene            complement(924..2096)
                     /locus_tag="VF720_09390"
     CDS             complement(924..2096)
                     /locus_tag="VF720_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7879610.1"
                     /translation="MPRTSLYFAAAFGVDGAVNLTLVGVPWKAIALGATPLELGLLPA
                     VWSVVYILSAMAMGHLSDRAPRLMLSGFGAMVVAFGLVLLAVADTLVGMQIGLALQAL
                     GLAMFWPSLQASLAGAVEPARLPRNLGWFNLSWSSGKGIGFAAGGLLYALHGFGLIAT
                     ISATITLAIGVLLFLAPRAQTAAGAGHGYPWPGPPMTTAPRIDPAKAAAFLAAAWVAN
                     GVAYGVVATLAYHYPKFLEAQGFRSAHFGFFLGGTYLSQTLMFALLMRTEKWHFRRGG
                     LYLMHAALALVLLLLPRVHAPALQLALAPIIGACLGLAYYSSIYYSLHAAARPGRNTG
                     RHEAIIGSGVLLAPPIGGWLATATGQLAAPYWLCAALSLLAIGGEQWLLGRRRSAT"
     gene            complement(2097..3014)
                     /locus_tag="VF720_09395"
     CDS             complement(2097..3014)
                     /locus_tag="VF720_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HEX7879611.1"
                     /translation="MLNSPPRTITTTVERDPASVRLRHHLDRLHATFDARWLSPDPLE
                     IVRRYDDPADQEVVGFLAAGLAYGRVGQILLAATNLLDRMSPSPARFIRDFDPSRDAA
                     RLRGWYHRFHGPRDMTLLLTVLSRTLARFGTLEAAFANGDDPASPDIEQGLDVFCRLL
                     LDTPDLPAGPGVSRGRLAPRAPVRFFLPTPADGSACKRLNLWLRWVARPADGLDLGLW
                     RRVDPARLIIPLDTHVARIARYVGLTARTSADWKTARDITSRLAQFDPADPVKYDFAI
                     CRLGILDRCPKRRDPGHCAECLLAPVCTL"
     gene            3117..4205
                     /locus_tag="VF720_09400"
     CDS             3117..4205
                     /locus_tag="VF720_09400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7879612.1"
                     /translation="MATRTRMETGVRAGTPTMAAPAVSVIVPCRNGAHHLRRTLPALR
                     EALPDGWEILLVDDHSSDASVEVGKLYADRVITSRQERSGPAARNSGALVARGGVFIF
                     MDQDIRVSRETLERLVEGLDEPGTALTFAVYSEGRYLKTIGGRFKNIWVRWSYLASPT
                     DVRWMNTALAAIRADDFWSAGAYDNEDDWVEGGNDIDFGRLVADRVGRVRLCPHLDCD
                     HLKEMSLWGLLRNDFNRTRGFFRMSMQSSEIKRVARGRWYGNISPTFMGGVTAAGLAP
                     VAALAWLAGLPWSGSVAIGAVALQLVLALPFLAYAMPRLGVIAPLAPIIYLFDQAACC
                     LGLATETVTLLGRRLFSRSWRTAPSPRF"
     gene            4175..>5512
                     /locus_tag="VF720_09405"
     CDS             4175..>5512
                     /locus_tag="VF720_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879613.1"
                     /translation="MAHRPLPAVLIAAALAAAGMAVAVAISFLPPSNWAWGIDLGRYY
                     PAWARGTWLAIGAALLAMAALGWPARSGGNGRPPLGLMLAWPAPLIGLVAGAMAGLIF
                     HLARARTGLLGDGYQILVDLRQGNPATARSTLYNVLEPMVVRLQRSGGVPTLTEAGLL
                     STLVGALTVAIVVTMLVRRARERSRPGGSTAVMAALLLTGTWLQLFFGYVECYAFLAS
                     AVLVFLVGALDRVEGRGSMAVPFAALVGAIASHPFGLLLVPAFLVIASIGPAEGRVRR
                     LAAWTGALVLLAGAGWFIASVWPKGADGRSPVEYLSPFRVLRQTRTLVMESTDIRDVM
                     RLDSVFSWPQLADWFNHLWLTGAAALPVLGALLFDARGRRALVSPAARVALVTTAAFA
                     LARLGMRTILGPMRDWDLFAPVGIGLAAWAAFAAADWIEAEVGNDEERDIEHAP"
BASE COUNT          847 a         1826 c         1995 g          844 t
ORIGIN      
        1 cggtactgcc acagctcacc atcccgcgta agaacccccc ccacgccgaa gctggtgacc
       61 aggccgcccc ggtctccggg tttggcgggt gacttcgcgt ccgagggggg agccagccag
      121 cgccccccga ccatggccgc gagggtaagg atggcgccaa gcacgacgcc gggccagaat
      181 ttcttgtaca tcgttggtac ctccgggccc gggtcagtga tgccgggcgg tggtatggcg
      241 ggtcatgacg tccaggtcgc ggatgatcct ggccccgagc cccctgacgg tttccagcca
      301 ctcgtcccgc gcttcgggct ccgcggacct cagggcgacg gcgttctgga ccacgtcgac
      361 ggtgatcccc tttgacttga gcgcccggac agcgttcagg acggcctctt ccccaccgac
      421 gccgaagacg atggcacccc gcacccaggt gaggatttcc gccgtgcccg ggttggacat
      481 cagatcgggc ccaccggttt ccacgacgat ctcccgggca tcgccgatga gcgctttgag
      541 ctcgccactt cccatgggcc gggccggcac caccaccggc ttcccgggcc gggtggcggg
      601 gatcttccgg gcaccgggtg aacccgccac gcaatagggc ggcggcattc cgtcggcgct
      661 gccgaagcgg ggatcattcg acgccagcgc ctggcaggtg gccacgacgc ggatgtcgta
      721 cgtgcgggcg taacgggtga gacgttcgaa gacagggttc aggccgaacg cgccaggcac
      781 cggccaggcg ccgtcgggca tcaccaggtc ccactgggtg tcgatatcca ggatgatcgt
      841 gtcggtgatc acggggtcgg gctcgctttc gttgagggcc agggggaagg cccggtcacg
      901 ggccatcgag ccggacgatg gtatcaggtg gcggaccggc gacggccgag gagccactgt
      961 tcgccgccga tggccaggag cgacagggcg gcacagagcc agtaaggggc ggccagttga
     1021 ccggtggccg tggccagcca acccccgatc ggcggcgcca ggaggacgcc ggacccgatg
     1081 atggcctcgt gccggccggt gttgcggccc gggcgggcgg ccgcgtgaag gctgtagtag
     1141 atgctcgaat agtaggcaag gccgaggcat gccccgatga tcggcgccag ggccagctgc
     1201 agtgccggcg cgtggacccg ggggaggagc aggaggacga gggccagcgc ggcatgcatg
     1261 aggtagagac cgccgcgccg gaagtgccac ttctcggttc gcatgaggag ggcgaacatg
     1321 agggtctgcg aaaggtaggt cccccccagg aagaatccga agtgggccga ccggaatccc
     1381 tgagcctcca ggaatttcgg atagtgatag gccagcgtgg ccacgacccc gtaggccact
     1441 ccgttggcca cccaggccgc ggcgagaaaa gcggcggcct tcgcgggatc gatacgtggt
     1501 gcggttgtca tgggaggccc gggccaagga tatccatgcc ccgccccggc cgcggtctgc
     1561 gcccggggcg cgaggaacaa cagcacgccg atcgccagtg tgatggtcgc cgaaatcgtc
     1621 gcgatcaaac cgaaaccatg gagtgcgtac aggaggccgc cggccgcgaa gccgatcccc
     1681 ttcccgctgc tccaggagag gttgaaccac cccaggttcc tgggaaggcg ggcgggctcg
     1741 acggcaccgg ccagcgatgc ctggagcgaa ggccagaaca tggcgagccc cagggcctgg
     1801 agggcgagcc cgatctgcat gccgaccagc gtgtcggcaa cagccaggag gaccaagccg
     1861 aaggccacga ccatcgcgcc gaaacccgag agcatcagtc ggggggcccg atccgagagg
     1921 tgccccatgg ccatcgcgga gaggatgtag acgaccgacc agacggccgg caacagcccc
     1981 agctcgagcg gtgtggcgcc cagggcgatg gctttccagg gtacgccgac gagcgtgagg
     2041 ttgacggcgc catccacgcc gaacgccgcg gcaaagtaga gagaggtccg gggcatctac
     2101 aacgtgcaga cgggcgcgag gaggcactcg gcacaatggc ccggatcgcg tcgtttcggg
     2161 caccggtcga ggatccccag acggcagatg gcgaaatcgt acttgacggg atcggcggga
     2221 tcgaattgcg ccagccgcga tgtgatgtca cgcgccgtct tccagtcggc cgaggtgcgc
     2281 gccgtgaggc cgacgtaccg ggcgatgcgg gccacgtgcg tgtcgagggg aatgatgagc
     2341 cgggccggat cgaccctcct ccagagtccc aggtcgagcc cgtcggccgg ccgggccacc
     2401 caccgcagcc agaggttgag ccgcttgcag gcactcccgt ccgccggggt cggcaggaag
     2461 aaacgaaccg gcgcccgcgg ggcgagccgc ccgcgtgaaa cgccggggcc ggccggaagg
     2521 tcgggcgtgt cgagcagaag gcggcagaag acgtcgaggc cttgttcgat gtccggtgag
     2581 gccgggtcgt cgccgttcgc gaaggcggcc tccagcgtgc cgaaccgggc cagggtgcgc
     2641 gaaagcacgg tgagcaggag cgtcatgtca cgcgggccat ggaatcggtg ataccagccc
     2701 cgcaatcggg cggcatcccg tgacggatcg aagtcccgga tgaaccgcgc cgggctgggg
     2761 gacatgcgat cgagaagatt cgtcgcggcg aggaggatct ggccaacccg accataggca
     2821 agccccgccg caaggaagcc caccacttcc tggtcggccg ggtcgtcgta gcgtcgcacg
     2881 atctcgagtg gatcggggga gagccagcgg gcatcgaacg tggcgtggag gcgatcgagg
     2941 tgatggcgca ggcgcacgga agccggatcg cgctcgacag tggtggtgat cgtccgtggc
     3001 ggggagttga gcatgtcgga agcctacgca acggggcgct cctcctgcta gactgcgcgc
     3061 tcgtcgacac gaatccgcca cggaagggcg gcctggatcc ggttcggaga gatcgagtgg
     3121 ccacacgcac gcgcatggag acgggcgttc gcgccggaac gcccacgatg gcggcgccgg
     3181 ccgtcagcgt catcgtcccc tgtcgcaacg gggcccacca tctccgccgc acgctccccg
     3241 cattgcgcga ggccctcccg gatggctggg aaatcctgct cgtcgatgat cactccagcg
     3301 acgcgagcgt cgaggtcggc aagctctacg ccgaccgggt gatcacgagt cgccaggagc
     3361 ggagcggccc cgcggcgcgg aacagcggtg cgctggtggc gcgtggcggc gtcttcatct
     3421 tcatggacca ggatatccgg gtaagccgcg agacgcttga gcgcctcgtg gaagggctgg
     3481 acgaaccggg taccgcgctc accttcgcgg tgtactccga ggggcgttac ctcaagacga
     3541 tcggcgggcg tttcaagaat atctgggtgc gctggtcgta cctggccagc ccgacggacg
     3601 tccgctggat gaacacggcc ctggccgcga tccgggccga cgacttctgg tccgccggcg
     3661 cctacgacaa cgaagatgac tgggtcgaag gaggcaatga catcgacttc ggccgcctcg
     3721 tggccgaccg ggtggggcgg gttcgcctct gcccccacct cgactgcgac cacttgaagg
     3781 agatgagtct ctggggactg ctccgcaacg acttcaatcg cacgcgcggc ttcttccgca
     3841 tgtcgatgca gtcgagtgaa atcaagcggg tggccagggg tcggtggtac ggaaacatct
     3901 cccccacctt catgggggga gtgaccgcgg ccggactggc gccggttgcc gcactggcgt
     3961 ggttggccgg cctcccgtgg tccggctccg tggccatcgg ggcggtggcg ctgcagctgg
     4021 tgctcgcgct tccgttcctt gcctacgcga tgccccggct cggcgtgatc gccccgcttg
     4081 ccccgatcat ctacctgttc gaccaggccg cctgctgcct cggtcttgcg acggaaaccg
     4141 tcacgctgct cggccggcgc ctcttctcac gttcgtggcg caccgccccc tccccgcggt
     4201 tctgatcgcc gcggcgctgg ccgccgccgg aatggccgtt gccgtggcca tttcattcct
     4261 gccgccgtcc aactgggcgt gggggatcga cctcggccgc tattatccgg cctgggcacg
     4321 tggaacctgg ctggcgatcg gcgcggccct cctcgcgatg gcggcgctgg gttggccggc
     4381 gcggtccggc ggcaacggcc ggcctccact cgggctgatg cttgcctggc cggctccgct
     4441 catcggcctt gttgccggag cgatggccgg cctgatcttt cacctggcgc gcgcccgcac
     4501 cggcctcctc ggcgacgggt accagatcct cgtcgatctc cgtcagggaa accccgcaac
     4561 ggctcgctcg acgctctaca acgtgctcga accgatggtc gtgcgtctcc agcgttcggg
     4621 cggagtgccg acgctcaccg aggccgggct cctctcgaca ctggtcggcg cgctgaccgt
     4681 ggcgatcgtc gtgacgatgc tcgtgcgacg cgcgcgggaa cgatcgcgcc ccggggggtc
     4741 gaccgccgtg atggcggcgc ttctcctgac cggtacgtgg ctccagctct tcttcggcta
     4801 cgtggagtgc tatgcctttc tcgcctcggc ggtcctggtc ttcctggtgg gcgcgctcga
     4861 ccgggtggaa ggacggggtt cgatggcggt gccgttcgcg gcgctcgtcg gcgcgattgc
     4921 ctcgcacccg ttcggtttgc tgctggtgcc ggccttcctc gtcatcgcct cgatcggacc
     4981 ggcggaaggg cgggttcgcc gattggccgc gtggaccggg gcgctggtgc tgcttgccgg
     5041 cgccggatgg ttcatcgcct cggtgtggcc gaagggcgcc gacggtcgat caccggtcga
     5101 atatctctcc ccgttccgcg tgctgcgcca gacccggacc cttgtcatgg agagcaccga
     5161 catccgggac gtgatgcggc tcgattcggt cttctcgtgg cctcaactcg ccgactggtt
     5221 caaccatctc tggctgacgg gagccgccgc gctcccggtc ctgggcgcgc tgctgttcga
     5281 cgcgcgtggc cggcgcgccc tggtgtcacc ggcggcgcgc gtggcgctcg tcacgaccgc
     5341 ggcgttcgcg ctggcccgcc tggggatgcg gacgatcctg gggccgatgc gcgactggga
     5401 cctgttcgcg ccggtcggga tcgggctggc ggcctgggcc gcgttcgcgg cggccgactg
     5461 gatcgaggcg gaagtcggca atgacgagga gcgcgacatc gaacacgcgc cc
//
LOCUS       DASVOD010000410         2396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_236474, whole genome shotgun sequence.
ACCESSION   DASVOD010000410 DASVOD010000000
VERSION     DASVOD010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236474"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VF720_09410"
     CDS             complement(<1..1150)
                     /locus_tag="VF720_09410"
                     /inference="COORDINATES: protein motif:HMM:NF039309.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX7879614.1"
                     /translation="MPRRRRRLKPILAIFSLFAFLGLGLSVRIFRGPYKDTSGLLTPV
                     IPVAVTQRPGCLEVGNVPRGSTLAEVLEDEGVRPEEAREWVGRLGSLFDLRRVRTEHQ
                     YRILSHEDGSLMRLEFQPDDSGFYVVEADSTGTQLNAWHENEPFDRRYRKVGGKVSGS
                     LYTSMVKQGLDAGLVSTFCDVFSYDVDFATETKDGDTFECLVEERLRDGRPFGTMRLL
                     SGQYKNGPEVVQAYWYEPSAGKGGWYRADGQALKRAYLKSPLNYTRISSGFTQNRLHP
                     IFKTVRPHLGVDYAAPTGTPVVAVADGSVESVGWVNGYGKLVVVRHANGVETYYAHLS
                     NYGRGLKAGASVNQNQVIGYVGQTGHATGPHLDFRVAVNGRFVNPLTFK"
     gene            complement(1308..>2396)
                     /locus_tag="VF720_09415"
     CDS             complement(1308..>2396)
                     /locus_tag="VF720_09415"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX7879615.1"
                     /translation="DIGYSDARHPDTVAFVRSIEEDLARRDFTVNAMAFSPAAGRFLD
                     PFGGLDDLGRGMIRAVGDPSIRFREDGLRAFRAVRLAVQLGFEIEERTLAAIPGALGS
                     ARQVAAERMRDEFSKMLVSGAPAMAVELMRRTGLLALVVPELLEGVNCRQNRWHAYDV
                     YAHSLHVLENAVPDKLEVRLAALLHDIAKPRTKVVVDGEGTFYNHQHLGARMAIDILD
                     RLRYPGDVVARVANLVDNHMFHYDGHWTDAAVRRFIRRVGPEQIADLFDLRLADSLGK
                     GPDGFFPKEIDELRGRVEAEIARAAVLTVGQLAVTGRDVMAALGVAGGPAVGEALRWL
                     LERVIEDPALNDRDRLLKILTNEYPQRA"
BASE COUNT          389 a          827 c          805 g          375 t
ORIGIN      
        1 acttgaaggt gagcgggttc acgaaccggc cgttgacggc cacgcggaaa tcgaggtgcg
       61 gccccgtggc gtgccctgtc tgccccacgt agccgatcac ctggttctgg ttgaccgaag
      121 cacccgcctt gagcccccgc ccgtagttcg acaggtgcgc gtagtacgtt tccacgccgt
      181 tggcgtgacg gacgacgacc agcttgccgt agccgttcac ccagccgacc gactccacgc
      241 tcccgtcggc caccgccacc accggcgtgc cggtgggggc cgcgtagtcg acacccaggt
      301 gggggcgcac cgtcttgaag atcgggtgga gccggttctg ggtgaagccc gaggagatgc
      361 gcgtgtagtt cagcggcgac ttgaggtagg cgcgcttgag cgcctggcca tcggcacgat
      421 accagccacc cttgccggcc gacggctcgt accagtaggc ctgcaccacc tcggggccgt
      481 tcttgtactg ccccgagagg aggcgcatgg tgccgaaggg gcggccgtcg cggagccgct
      541 cctcgaccag gcattcgaac gtgtcgccat ccttcgtttc ggtggcgaaa tcaacgtcgt
      601 agctgaagac atcgcagaac gtggacacga gccccgcgtc gagcccctgc ttcaccatgc
      661 tggtgtagag ggagccggag accttccccc cgaccttccg gtagcgacgg tcgaacggtt
      721 cgttctcgtg ccaggcattg agctgggtgc cggtggagtc ggcttccacc acgtagaagc
      781 cgctgtcgtc gggctgaaac tcgagccgca tcagcgagcc gtcttcatgc gagaggatgc
      841 gatactggtg ctcggtgcgg acgcggcgca ggtcgaagag cgatccgagc cgccctaccc
      901 actcgcgcgc ctcttccggc ctgacgcctt cgtcctcgag cacctcggcc agcgtggagc
      961 cgcgagggac gttccccacc tccaggcaac ccggtcgctg cgtgaccgcc acggggatga
     1021 ccggcgtgag gaggcccgag gtgtccttgt agggaccccg gaagatccgg accgagaggc
     1081 ccaggcccag gaaggcaaac aggctgaaaa tggccagaat gggcttgagc cgccggcgtc
     1141 gtctcggcat cgcactcccg tacgaagggt cgccagccgg cggcccgtca tcttttgagg
     1201 ttcggctatc gtcgtgccgg ggaccggaac ggcgaggagg ggtgacaagt tgttgcggcc
     1261 ggcccggata gatcgacggg gactctatgg gggcttcccc catactgtca agccctctgg
     1321 ggatattcgt ttgtcaaaat tttcagcagg cggtcccggt cgttcagggc cggatcctcg
     1381 atgacgcgct cgaggagcca tcgcagggcc tcccccaccg ccgggccccc cgccacgccg
     1441 agggcggcca tcacgtcccg gccggtcacg gccagctgcc ccacggtcag aacggcggcc
     1501 cgggcaatct cggcctccac ccgcccgcgc aactcgtcga tctccttcgg gaagaacccg
     1561 tccggcccct tccccagcga gtcggccagg cgcaggtcga agagatccgc tatctgctcc
     1621 ggccccacgc ggcggatgaa gcgccgcacc gccgcgtcgg tccagtggcc gtcgtagtgg
     1681 aacatgtggt tgtcgaccag gttggccacg cgcgccacga catcgcccgg atagcgaagc
     1741 cgatcgagga tgtcgatcgc catccgggcg ccgaggtgct ggtggttgta gaaggtcccc
     1801 tcaccgtcca ccaccacctt cgtgcgcggc ttggcgatgt cgtggagcag cgcggcgagg
     1861 cgcacctcga gcttgtcggg aacggcgttc tcgagcacat gcagcgagtg cgcgtacacg
     1921 tcgtacgcgt gccacctgtt ctgccggcag ttcacccctt ccaggagttc cggtaccacg
     1981 agtgccagaa ggccggtccg ccgcatcagc tcgacggcca tggccggcgc ccccgacacg
     2041 agcatcttcg aaaactcgtc gcgcatccgc tcggccgcca cctgccgggc cgagccgagc
     2101 gcgcccggga tggcggccag cgtccgctcc tcgatctcga agccgagctg caccgccagg
     2161 cgcaccgcgc ggaaggcccg gagcccatcc tcgcggaagc ggatcgacgg atcgccgacg
     2221 gcgcggatca tcccgcgccc caggtcgtcg agtccgccga acggatcgag gaaacgcccg
     2281 gccgccggtg aaaacgccat ggcgttcacg gtgaagtcgc gccgggccag gtcttcctcg
     2341 atggagcgga cgaaggccac ggtgtcggga tgccgggcgt cggaatagcc gatgtc
//
LOCUS       DASVOD010000411         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_80656, whole genome shotgun sequence.
ACCESSION   DASVOD010000411 DASVOD010000000
VERSION     DASVOD010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80656"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF720_09420"
     CDS             complement(<1..288)
                     /locus_tag="VF720_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879616.1"
                     /translation="MSFDIVDSQAAARQLAAAHPGAWPDALPVLDALTTRGHDAFLVG
                     GTVRDVLLGRSPGDPDIATDAIPERMMEFLPNPKPTGLRHGTVTSVVNGEHI"
     gene            complement(317..1753)
                     /locus_tag="VF720_09425"
     CDS             complement(317..1753)
                     /locus_tag="VF720_09425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7879617.1"
                     /translation="MSLNRNVQAYIGLIVALAGIALVSGSVQIRTFEWQTLALWSMVC
                     VVAELLWLNTLTGQGTVSMASTFNLAIIFLLGWEKSLWVAGASTLFANLAIQKKEWYK
                     ALFNTAQSILSTAIAGILFLLTGGVTLLPDADSSGVQQIASFLSRFNDARLIIPFLAC
                     GLAYHFANTFFVSGVISLASKGRLLPTWRENYGYSEEIVSSLALIFLSPLVVLSFGAV
                     SFLGIILFFVPLLFVRDASRRYLELMRTQDAMIKAAALAARGEMAAKMAHELNNYLAA
                     ISARAQMILMYQPIDGDPRTRKSGEIIYENITKMKRLVDDMVRPAATHMRRTVNLNSV
                     VEQAVDLSRFHERFDDVSIDVVLDPAVPPGQFDDMQIQQVIMNLLKNAGEAMLGADIV
                     GKRVEVRTAFDAERNQVILTVTDNGPGMPTEVAARIFKTSFTTKADGHGLGLSTSNKI
                     IQGHDGRIVVESRPGAGATFTITLPLAA"
     gene            complement(1850..3514)
                     /locus_tag="VF720_09430"
     CDS             complement(1850..3514)
                     /locus_tag="VF720_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004937385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7879618.1"
                     /translation="MRSIRGISMGEPLFSVSGLSVEFPSAEGAPRRILDNVSFDVGVG
                     ESLGVVGESGSGKTMTALAVMGLLPFPGRVTAGAIRWEGKDLVSAPVEERRRLRGASM
                     GMIFQEPLSSLNPVMTCGDQVVEALRVREGMTAAAARRRGVELLDRVGIGDPARRALD
                     YPHQLSGGMRQRVMIAMALAMSPRLLIADEPTTALDVTIQAQILDLLAKLCAEEKLAL
                     LLITHNFGVVARLTQRVVVMNNGKVVEAASTAALLAAPREAYTKALLAAVPRLDGPRR
                     AGHVSARASGAPMLELENVSRTFRLGRSSFMGKPRTLDAVRDVSLTVRRGETLGLVGE
                     SGSGKTTLGRMILRLLKPTAGRVVVDGTDVAALSDRALRPLRRRMQMVFQDPAGSLNP
                     RLTVESALVEPLEVHGLLRGAAARRRVGELLEQVGLPADAARRYPHQFSGGQRQRIGI
                     ARALALGPELLVLDEPVSALDVSVQAQILDLFVRLRKDLDLTSIFIGHDLAVVRLVCD
                     RVAVMYRGEIVESGDVETVYANPLHEYTRSLLAAVPRLTPGSAPPA"
     gene            complement(3483..4562)
                     /locus_tag="VF720_09435"
     CDS             complement(3483..4562)
                     /locus_tag="VF720_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7879619.1"
                     /translation="MAEEVLAKRGIPTSPGAIVWWRLKRNRPAWLAWCVLVVLYGLAL
                     VAPILAPYGESSGERTRPYEAPSRLVWSGGPAFQSMAKVGRAWQPAGSPVRLGIFVRG
                     DAYEILPGVRFDRHLYGAIGAPGTERVYLLGADQFGRDVFSRILYGARVSLSVGLIGV
                     ALSFTLGLLVGGWAGYRGGITDSLLMRGSEILMSIPALYLILALRTAFPASLPSSLTY
                     LMIVLILSLVSWTGLGRIVRGMVLSLRTRDYVSAAEALGLSPVRILVRHLLPNTLSFA
                     VVAATIAVPGYILGEVALSFLGVGIQEPAASWGNMLQQAASVSVMESSPWILWPGAAI
                     FVTVLAFNVLGDGLRDALDPRHLDG"
     gene            complement(4555..>5414)
                     /locus_tag="VF720_09440"
     CDS             complement(4555..>5414)
                     /locus_tag="VF720_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7879620.1"
                     /translation="PSVSRESLEGMRREFGLDKPAWQQYGIYLKNILLKGDFGYSFSR
                     RQPVFTVLGESLGNTLVLSLAAALLTWLLAVPLGVLAAVKQHSWVDRLASGASMVGLA
                     IPEVFLALLLMLFAYKTGWFPTNGMRSIDHDSLSWIGRMGDIGHHLVLPALVLATVPL
                     ASRTRQMRANLLDVLRADYVTTARAKGLTESRVVMRHALPNALNPLITLFGYTLGGLL
                     SGSFLVENVMGWPGLGRVAIEALNTRDLYLVMGSVLMASTVLILGNLVADVLLALSDP
                     RIRVGSSDG"
BASE COUNT          993 a         1923 c         1748 g          750 t
ORIGIN      
        1 gatgtgctcg ccgttcacca cgctggtcac cgtgccgtgc ctgaggccgg tgggcttggg
       61 gttcgggagg aattccatca tccgttcggg aatggcgtcg gtggcgatgt cgggatcacc
      121 gggggaacgg ccgaggagga catcgcggac ggtgccgccg acgaggaagg cgtcgtggcc
      181 gcgggtggtc agtgcatcga ggacgggcaa ggcgtccggc caggcgccgg gatgagcggc
      241 ggccaattgc ctcgcggcgg cctgggagtc aacgatgtcg aaggacatga tcgagatgct
      301 aacgcatctc tccgtatcag gcggccagcg gcagggtgat cgtgaacgtg gccccggcgc
      361 cggggcgcga ctccaccacg atgcgcccgt cgtgtccctg gatgatcttg ttgctggtgg
      421 agaggccgag gccgtggccg tcggccttgg tggtgaagct cgtcttgaag atccgggcgg
      481 ccacctccgt gggcatgccg ggaccgttgt cggtgacggt caggatcacc tggtttcgct
      541 cggcgtcgaa ggcggtgcgc acctccaccc tcttgccgac gatgtcggct ccgagcatcg
      601 cttcgcccgc attcttgagg aggttcatga tcacctgctg gatctgcatg tcatcgaact
      661 gcccgggggg aacggccggg tcgagcacca cgtcgatcga cacgtcgtcg aaccgctcgt
      721 ggaagcgcga gaggtccacc gcctgctcca ccacgctgtt caggttcacg gtccgccgca
      781 tgtgcgtggc agccgggcgc accatgtcgt ccaccagccg cttcatcttg gtgatgttct
      841 cgtagatgat ctcgccgctc ttgcgggtgc gcggatcgcc gtcgatcggc tggtacatga
      901 ggatcatctg ggcccgcgcg gaaatggcgg ccaagtagtt gttcagctcg tgggccatct
      961 tggcggccat ctcgccgcgg gcggccaggg cggccgcctt gatcatggcg tcctgcgtgc
     1021 gcatcagttc caggtagcgg cgcgaggcgt cacgcacgaa cagcagcggc acgaagaaga
     1081 ggatgatgcc gaggaacgag acggcgccga acgagagcac caccaggggc gacaggaaga
     1141 tcagggccag cgaagagacg atctcctcgg agtagccgta gttctcgcgc cacgtcggga
     1201 gcagccgccc cttcgaagcc agcgagatga cccccgacac gaagaaggtg ttggcaaagt
     1261 gatacgccag gccgcatgcc aggaacggga tgatcaggcg ggcgtcgttg aagcgcgaga
     1321 ggaacgaggc gatctgctgc acgccggagg agtcggcgtc gggcaggagc gtcacgccgc
     1381 cggtgagcag gaagaggatg cccgcgatgg cggtcgacag gatggactgc gccgtgttga
     1441 agagcgcctt gtaccactcc ttcttctgga tcgccaggtt cgcgaagagc gtggaggcgc
     1501 cggccaccca gagtgatttt tcccaaccga gcaggaagat gatggccagg ttgaacgtgg
     1561 aggccatgct cacggtgccc tggccggtga gggtgttgag ccacaggagc tccgccacca
     1621 cgcacaccat cgaccagagc gcgagcgtct gccactcgaa cgtgcggatc tgcacggagc
     1681 cggacacgag cgcaatgccc gcgagcgcca cgatcagtcc gatgtaagcc tggacgttcc
     1741 ggttgagcga caaggacccc gatccccgag gagaatggaa agtgcgttta ccccacggag
     1801 ttatcggctg ggtgggaaat aacttaatac cggatggggg gattcaccct caggccgggg
     1861 gggccgaccc gggggtcagg cgcggcacgg cggccagcag ggacctcgtg tattcgtgga
     1921 ggggattggc gtaaaccgtt tccacgtcac cgctttccac gatctcgccc cggtacatca
     1981 ccgccacccg gtcgcagacg agtcgcacga ccgccaggtc gtgcccgatg aagatggagg
     2041 tgaggtccag gtccttccga agccggacga acaggtcgag gatctgggcc tggacggaca
     2101 catccagcgc cgacacgggc tcgtcgagca ccagcagctc cggccccagc gcgagcgccc
     2161 gcgcgatgcc gatccgctgc cgctgtcccc ccgagaactg gtgtggatag cgccgggccg
     2221 cgtcggccgg cagccccacc tgctcgagca actcccccac acgtcggcgc gccgcggcac
     2281 cgcgaagcag gccatgaacc tcgagcggct cgaccagcgc cgactccacg gtaaggcgcg
     2341 ggttgagcga accggccgga tcctggaaca ccatctgcat ccgacgtcgc aaagggcgca
     2401 gcgcccgatc ggataatgcg gccacgtcgg ttccatccac caccacccgg ccggccgtcg
     2461 gcttgaggag gcgcaggatc atccgaccga gcgtcgtctt ccccgagccc gattccccga
     2521 cgaggccgag cgtctcgccg cgccgcacgg tcagcgacac gtcgcgcacc gcgtcgagcg
     2581 tgcgcggctt gcccatgaag ctggaacgcc ccagccggaa cgtgcgcgac acattctcga
     2641 gttcgagcat gggcgctccc gaagcccgcg ccgatacatg ccccgcccgc cgcggtccgt
     2701 cgagccgcgg tacggcggcc agcagcgcct tcgtgtacgc ctcgcggggc gcggccagga
     2761 gcgcggccgt ggaagccgcc tccacgactt tcccgttgtt catcaccacc acgcgctgcg
     2821 tcagccgcgc caccacgccg aagttgtggg tgatgaggag gagcgccagc ttttcctcgg
     2881 cgcacaattt ggccagcagg tcgaggatct gggcctggat cgtcacgtcg agcgccgtgg
     2941 tcggctcgtc ggcgatgagc aaccgcggcg acatagccag cgccatggcg atcatcaccc
     3001 gctggcgcat cccgcccgac agctggtgcg gatagtcgag cgcccgtcgc gccggatcgc
     3061 cgatcccgac ccgatcgagc agctccaccc cccgccgccg cgccgcagcc gccgtcatcc
     3121 cctcgcggac gcgcagcgcc tccaccacct ggtcgccgca cgtcatgacg ggattgagcg
     3181 acgacagcgg ctcctggaag atcatcccca tcgaggcgcc gcgcagccgg cgccgctcct
     3241 cgaccggggc cgaaacgaga tcctttcctt cccagcggat ggcccccgcg gtcacccgcc
     3301 ccgggaaggg caggagcccc atcacggcca gcgccgtcat cgttttgccc gaccccgact
     3361 cccccaccac gccgagcgat tccccgacgc ccacgtcgaa cgacacgtta tccaggatcc
     3421 ggcgcggggc gccttccgcc gacggaaact cgacggagag ccccgacacg gagaagagcg
     3481 gctcacccat cgagatgcct cggatcgagc gcatcgcgca ggccgtcacc cagcacgttg
     3541 aaggccagca cggtcacgaa gatggccgcc cccggccaga ggatccacgg cgacgactcc
     3601 atcaccgaca cgctggccgc ctgctgcagc atgttccccc aggaggcggc cggctcctgg
     3661 atgcccacgc ccaggaacga cagcgccacc tcgccgagga tatatcccgg caccgcgatc
     3721 gtggccgcca ccaccgcgaa cgacagcgtg ttcggcaaca ggtgccgcac caggatccgc
     3781 accggcgaca gccccaatgc ctcagccgcc gacacgtagt cgcgcgtgcg gagcgacagc
     3841 accatgccgc gcacgatccg cccgaggcca gtccacgaca ccagcgagag gatgagcacg
     3901 atcatgagat acgtcagcga ggacggcagt gaggccggga acgccgtgcg caatgccagg
     3961 atgaggtaca gcgccgggat cgacatcagg atttccgacc cgcgcatcaa caaagaatcc
     4021 gttatcccgc cgcgatagcc ggcccagccc cccacgagca gccccagcgt gaagctcagc
     4081 gccacgccga tcagccccac cgacaacgac acccgcgccc cgtacaggat gcgcgagaag
     4141 acatcccgcc cgaactggtc ggcccccagc aggtacaccc gctccgtccc aggcgccccg
     4201 atcgcaccgt acaggtgtcg atcgaaccgc acgcccggca ggatttcgta ggcatctccc
     4261 cgcacgaata tcccgagccg caccggcgaa ccggccggct gccacgcgcg ccccaccttc
     4321 gccatcgact ggaacgccgg cccgcccgac cacacgagcc tggacggcgc ctcgtacggc
     4381 cgcgtccgct caccgctcga ctcgccgtac ggcgccagga tcggcgccac cagcgccagc
     4441 ccgtacagca ccaccagcac gcaccacgcc agccacgccg gccggttgcg cttcagccgc
     4501 caccagacaa ttgcccccgg actggtcggg atgccgcgct tggccaggac ttcctcagcc
     4561 atcgctcgaa cccacccgga tccgcggatc cgacaaggcc agcagcacat cggccacgag
     4621 gtttcccagg atcagcacgg tcgatgccat cagcactgac cccatcacga ggtacaggtc
     4681 gcgcgtgttc agcgcctcga tcgccacccg ccccagcccg ggccacccca tgacattctc
     4741 gacgaggaac gaaccggaga gcagcccgcc cagcgtgtag ccgaagagcg tgatcaaggg
     4801 attcagcgcg ttcggcaacg catgtcgcat caccacgcgc gattcggtga gccccttggc
     4861 ccgggccgtc gtcacgtagt cggcccgcag cacgtcgagc agattggccc gcatctgccg
     4921 cgtacggctg gccaaaggca cggtggccag caccagcgcc ggcagcacca ggtgatggcc
     4981 gatgtccccc atccgcccga tccacgacag ggagtcatga tcgatggagc gcatcccgtt
     5041 ggtcggaaac cagccggtct tgtaggcgaa cagcatgagc aggagcgcga ggaagacttc
     5101 cggaatggcc agccccacca tgctggcccc cgacgccaat cggtccaccc acgagtgctg
     5161 cttgaccgcc gccagcaccc ccagcggtac cgccaggagc catgtcaaaa gcgccgccgc
     5221 cagcgacagc accagcgtgt tccccagcga ctcgccaagc accgtgaata cgggctgccg
     5281 ccgtgaaaac gagtagccga agtccccctt cagcaggatg ttcttgaggt agatgccgta
     5341 ctgctgccac gcgggcttgt ccagcccgaa ctcccgccgc atcccctcca gcgattcgcg
     5401 gctgaccgac ggat
//
LOCUS       DASVOD010000412         5710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_236634, whole genome shotgun sequence.
ACCESSION   DASVOD010000412 DASVOD010000000
VERSION     DASVOD010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236634"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3282)
                     /locus_tag="VF720_09445"
     CDS             complement(<1..3282)
                     /locus_tag="VF720_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879621.1"
                     /translation="MTLRSAIGPGIRFLLATASIIGFSTTDPTAVRAATDPTDTRPAA
                     DLRPTLTFAENRGQWPEAIHYAASVPGALIELGPTGATYVISRPTSTDSPTDPTHSAS
                     AKPAPLESIALRASFPGSRLDATLAPEQPAANLSHYYLGNDPTAWRTNVPNFGAIRYD
                     GLYPGIDLVYHGADGRLEYDFEVEPGADWRVIRVQYDGFEKLQITANGDLALTTRFGT
                     VIERAPVAWQVIDGARHPVRAGYRRMSKNTFGFEIFDPIDPAASLIIDPVLHYGTYLG
                     GSVWDEMHGVDYNFAGEAWVVGGTRSPDFPATGSPVPSTKYSVCLTRMAANGQSQVWS
                     VFLGGSEDQIGYGLDLDVNDNAYISGFTGSNNFPTTAGCYDATFNGTNPGETDLFVAK
                     LSSTGALLYSTYLGGTDSETNSGLVLGPSAQVYVSADAGQNYPTTAGCYDATQNGGSD
                     VVLSVLNPVGSGPADLVYSTYVGGSTYDESHGLAVDVSGYAWIAGATLSSDYPATGGP
                     PFNGTADAFITQIKPNGAGPADLGYSEKFGGTGNDYGLAVATEFIGVAFTGVTSSSNF
                     PVTSGVFQPTYGGGSSDAFVMFLLPSSNFVFYSTYLGGTGADQAGAAHLSFGGRITAA
                     GSTGSPNFPTTAGAYDTTPNGNVDMFLARFDFSGATLLEGTFIGGSGDDYLYDFAEDL
                     ISRRYVTGTTTSGNFPVTATAFDTSFGGSPIDGVVLKFQPPHPEVCAACASPPDTCCA
                     RAPKFLQAEPAPSLSPNVLVGTRNESTPTPYAVTVYDLGSPTPTEDVEWTTISRYNGP
                     GAGWLTSQLGTVFGLTLDEYGNIFVTHASCYDGDQVGTIPGAGPGAVYRIDANTGGVT
                     TFCRLPNYPDPNVIAGQNYPALGNISYDCRHKQFFVTNLEDGRIYRIKPNGPNGVTGT
                     IQEVFDPGVADTGPTNYLTPIGSVPSPGWAPLGERLWGVQWHMDRVYYGVWIEDISAP
                     SSTLANEVRSVALNAAGAFVTSSDQHELYVQPLSPWDYSMPVSDISFHTDGRMLLGER
                     GIDNTTATSPHNARSLEYRCEGGCWVSANQYQVGVCCAGANSAGGVDYDHFPYTGSQL
                     GRVWSS"
     gene            3564..4394
                     /locus_tag="VF720_09450"
     CDS             3564..4394
                     /locus_tag="VF720_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7879622.1"
                     /translation="MSITPVEESVYGTGGRLFVRSWSPVGPPRAILAICHGFNSHSGQ
                     YDWVAQQFTAAGYAVYAMDLRGRGKSEGERFFVDHIDEYVSDLSTLIQLARQRAPGLK
                     VFLLGHSAGGVISCTYTLDYQKELAGLICESFAFRVPAPKLALDAIKGISSFAPRLPV
                     LTLHNKDFSRDPAAVAALDADPLIAHETQPAETVAALWRADERLTKEFPKIHIPVFIM
                     HGTHDKATVPAGSQFFYDTVGSTDKTLRMYQDHFHDLFNDVGKETVMADTLAWVAARL
                     "
     gene            4399..4692
                     /locus_tag="VF720_09455"
     CDS             4399..4692
                     /locus_tag="VF720_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879623.1"
                     /translation="MSTGMFTPEQRQRLEGRVLTMVCRQRGLPAHFGRGFDELPAELL
                     EDCRDSLVRAPDREELLRALGSTIKLLLTASGEVPELAARVGPELNRLVTPWA"
     gene            4683..5081
                     /locus_tag="VF720_09460"
     CDS             4683..5081
                     /locus_tag="VF720_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879624.1"
                     /translation="MGLIPARRWTWAAPVMWLGLLGVGIALMGCHGESKTGQPAEVAS
                     RLLGGALRGDTAAVAAELTRATRARAGEVIAEVTRNDSLQRVEWVSTNIWSEQGANVD
                     VKRTFRGGRSDQLRLELKRERGDWRVAKAE"
     gene            complement(5082..>5710)
                     /locus_tag="VF720_09465"
     CDS             complement(5082..>5710)
                     /locus_tag="VF720_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879625.1"
                     /translation="ELKGDALTVAGQEGVMTFQRASSDMAVGLNATGKDADARKKASD
                     DIVVNGVKLTPAEITKFETQFHITMLAGTYWYDAKCGAWGSQDGPTVGFLPANLQLGG
                     PLKADASGRNPTGVFINGRQIHPVDVAALQQITAVLPGHYWVDAAGNCGFEGNPTPLA
                     NLVQLAQAARSQRGGTYHSRSDITGIGSGGDGKTSYVMGKDWSVIIGE"
BASE COUNT          927 a         1765 c         2006 g         1012 t
ORIGIN      
        1 ggaactccac acgcggccca actggcttcc ggtgtagggg aaatggtcgt agtccacccc
       61 accggcggag ttggcgccgg cgcagcagac gccgacctgg tactggttgg ccgacaccca
      121 gcaacccccc tcgcagcggt actcgaggct gcgggcattg tgcgggctgg tggcggtcgt
      181 gttgtcgatg ccgcgttctc cgaggagcat gcgtccgtcg gtgtggaacg agatgtcgga
      241 gaccggcatg gagtaatccc acggcgacag cggctgaacg tagagttcgt gctggtcgct
      301 cgacgtgaca aacgcccccg ccgcgttcaa cgctacgctg cgcacttcat tcgcgagcgt
      361 ggagctcggc gcgctgatgt cctcgatcca cacgccgtaa tacacgcgat ccatgtgcca
      421 ctgcacgccc cacaggcgtt ccccgagcgg cgcccagccc ggggagggca cgctgccgat
      481 cggcgtcagg tagttcgtcg gacccgtgtc ggcgacgccc ggatcgaaca cctcctggat
      541 cgttccggtc acgccgttcg ggccgttggg cttgatgcgg tagatgcggc cgtcttccag
      601 gttggtcacg aagaattgct tgtggcggca gtcgtagctg atgttgccga gggccgggta
      661 gttctggccg gcgatcacgt tcgggtcggg atagttggga agccggcaga acgtggtgac
      721 gccgccggtg ttggcgtcga tgcgatacac cgcgcccggg ccggcgccgg gaatcgtgcc
      781 gacctggtcg ccgtcgtagc acgacgcgtg cgtcacgaag atgttgccgt actcatccag
      841 cgtcagtccg aacacggtgc cgagctggct cgtgagccac ccggcacccg gaccgttgta
      901 gcgggagatg gtggtccact ccacgtcttc ggtgggcgtc gggctgccga ggtcgtacac
      961 ggttacggcg tacggcgtgg gcgttgattc gttacgcgtg cccacgagca cgttgggcga
     1021 aaggctgggc gccggctcgg cctgcaggaa tttcggcgcc ctggcgcagc aggtatcggg
     1081 cgggctggcg caggcggcgc acacctcggg atggggcggt tggaacttga ggaccacgcc
     1141 atcgatcggg ctgccgccga agctggtgtc gaaagccgtt gcggtcacgg ggaagttgcc
     1201 gctggtggtc gtgccggtca cgtagcgtcg gctgatcagg tcttccgcga agtcgtagag
     1261 gtagtcgtcg ccgctcccgc cgataaacgt tccctcgagg agcgtggcgc cggagaagtc
     1321 gaacctggcc aggaacatgt ccacattgcc gttgggtgtg gtgtcgtacg cgccggccgt
     1381 ggtggggaag ttgggcgaac cggtcgagcc ggccgcggtg atccggccgc cgaagctcag
     1441 gtgcgcggcc ccggcctggt cggccccggt cccgccgagg tacgtgctgt agaagacgaa
     1501 gttcgagctg gggaggagga acatcacgaa ggcgtcgctc gagccgccgc cgtaggtggg
     1561 ctggaacacg cccgatgtca ccgggaaatt ggaggagctg gtcacgccgg tgaaggccac
     1621 gccgatgaat tcggtggcca ccgcgagtcc gtaatcgttg cccgtcccac cgaacttctc
     1681 gctgtatccc aggtcggcgg gaccggcgcc gttcggcttg atctgggtaa tgaaggcatc
     1741 ggcggtaccg ttgaacggcg gcccgccggt ggccggatag tccgacgaaa gagtggcgcc
     1801 ggcgatccag gcatacccgg acacgtccac ggccagcccg tggctctcgt cgtacgtgct
     1861 tccgccgacg tacgtgctgt agacgaggtc cgcggggccc gacccgaccg gattgaggac
     1921 ggagaggacg acatcgcttc cgccgttctg cgtggcgtca tagcagccgg ccgtggtggg
     1981 atagttctgg cccgcgtcgg cggacacgta cacctgggcc gaggggccga gcacgagccc
     2041 cgagttggtc tcgctgtcgg tgcctcccag gtaggtgctg tagagcagtg cgccggtcga
     2101 ggacaacttg gccacgaaga gatccgtctc gcccggattg gtgccgttga acgtggcgtc
     2161 gtagcacccg gcggtcgtcg ggaaattgtt ggagcccgtg aagccgctga tgtacgcgtt
     2221 gtcatttacg tcgaggtcga ggccgtagcc gatctggtct tccgagcccc ccaggaacac
     2281 gctccacacc tgcgattgcc cgttggctgc cattcgcgtg aggcaaaccg agtatttcgt
     2341 gctcggaacg gggctcccgg tggccgggaa gtcgggcgag cgcgtgcccc ccacgaccca
     2401 ggcttcaccg gcgaagttgt agtcgacacc gtgcatctcg tcccacacgg agccgccgag
     2461 ataggtgccg tagtggagaa ccgggtcgat gatgagcgac gcggccggat cgatcgggtc
     2521 gaatatttca aatccgaacg tgttcttcga cattcggcga taaccggcgc gaaccggatg
     2581 ccttgcgccg tcgatcacct gccacgccac cggcgcacgt tcgatcaccg tgccgaaacg
     2641 cgtcgtcagc gcgaggtcgc cgttcgccgt gatctggagt ttctcgaaac cgtcgtactg
     2701 cacgcggatc acgcgccagt cggcgcccgg ttcgacctcg aagtcgtatt cgagccgccc
     2761 gtcggccccg tgatacacga ggtcgatgcc cgggtagagc ccgtcgtaac ggatggcgcc
     2821 gaagttcggg acgttggtcc gccatgcggt cgggtcattg ccgaggtagt agtgactcag
     2881 gttcgccgcg ggctgttcgg gtgccagcgt cgcgtccagg cgcgaaccgg gaaaggacgc
     2941 gcgaagcgcg atcgattcga gtggcgccgg cttcgcggaa gcggaatggg tggggtctgt
     3001 tggcgaatca gtcgatgtcg gccgtgagat cacataggtc gcgccggtcg ggccgagctc
     3061 gatcagggca cccggcacac tggcggcata gtggatggcc tcgggccact ggccccggtt
     3121 ctcggcgaag gtgagggtcg ggcgaagatc ggccgccgga cgggtgtcgg tcgggtccgt
     3181 cgccgcgcgg acagcagtgg ggtcggtcgt cgaaaaacca attatcgaag cagtcgcgag
     3241 gagaaatcga attcccgggc cgatggcgga gcgcagcgtc atcttcggac tccacggttg
     3301 ggtagggcga accggttcgg gagaggagct ccagtttagc gcatggtcgg gcgatcccca
     3361 tcttccaatt gagcgcagcg gggcagccgg ccggcaacga atcgtaagtg cttggcgggt
     3421 atggaggtgc aaactccgtg ggcgatcgcg gccaccgggc tgtttaccca gctggctaca
     3481 tccagcggct tttcctagac ttcgccatcc gagccttcga atttcctgca tgagacccgt
     3541 cacgagcccc ggaaggagat ttcatgtcca ttacgccagt ggaagaatcc gtctacggca
     3601 ccggtggcag gctgttcgtc cgctcgtgga gccccgtcgg cccgccgcgc gccatcctgg
     3661 ccatctgcca cggcttcaac tcgcacagcg ggcagtacga ctgggtggcg cagcaattca
     3721 cggcggccgg ttacgccgtg tacgcgatgg acctccgcgg tcgcggcaag tcggaggggg
     3781 agcgcttctt cgtcgaccac atcgacgagt atgtgagcga cctgtcaacg ctcatccagc
     3841 tggccaggca gcgtgcgccg ggcctcaagg tgttcctgct cggccacagc gccggcggcg
     3901 tcatctcctg cacctacacg ctcgactacc agaaggagct tgccggcctc atctgcgaaa
     3961 gtttcgcgtt ccgcgtgccg gcgcccaagc tggcgctcga tgccatcaag gggatcagca
     4021 gtttcgcgcc ccgccttccc gtcctcacgc tgcacaacaa ggatttctcg cgcgatcccg
     4081 ccgccgtcgc ggccctcgac gccgatccgc tcatcgccca cgaaacccag ccggccgaaa
     4141 ccgtcgcggc cctctggcgc gccgatgagc gcctgacgaa ggaattcccg aagatccaca
     4201 tccccgtgtt catcatgcac ggcacgcacg acaaggccac cgtccccgcc ggcagccagt
     4261 tcttctacga cacggtcggc tcgacggaca agacgctccg gatgtaccag gatcacttcc
     4321 acgacctgtt caacgatgtc gggaaggaaa ctgtcatggc cgatacgttg gcgtgggtgg
     4381 ccgcgaggtt gtaatcgaat gagtaccgga atgttcacgc ccgagcagcg tcagcgtctc
     4441 gaaggccgtg tactgacgat ggtgtgtcgc cagcgcggcc tgccggcgca cttcggtcgg
     4501 gggttcgacg agcttccggc ggaattgttg gaggactgtc gtgactcact cgtgcgtgcg
     4561 ccggatcggg aggagttgtt gcgggcgctc gggagcacga tcaagctgct gttgacggct
     4621 tccggcgagg ttccggaact ggccgccagg gtggggccgg aattgaaccg gttggtgacg
     4681 ccgtgggcct gatacccgca cgacgctgga catgggccgc tccggtcatg tggctcggtc
     4741 ttctcggggt tggcatcgct ctcatgggct gccacggcga gtcgaagacc ggtcaaccgg
     4801 ccgaggtggc cagtcgactt ctcggcggcg cgctgcgggg agacaccgct gcggtggcgg
     4861 ccgagcttac gcgagccacc cgcgcccgcg ccggcgaggt gatcgccgaa gtgacgcgga
     4921 acgactcgct ccagcgcgtc gaatgggtga gcacgaacat ctggtcggag caaggtgcga
     4981 acgtcgatgt gaagcggacg ttccgaggtg gacggagtga ccagttgcga ctggagctca
     5041 agcgcgagcg cggggattgg cgcgtggcga aggcggaata actactcccc gatgatcacc
     5101 gaccagtcct tccccatcac gtaactcgtc tttccatctc cgccggagcc gatgccggtg
     5161 atgtcggagc gcgagtgata cgtgccgccg cgctgggagc gggcggcctg ggcgagctgg
     5221 acgaggttgg cgaggggcgt tgggtttcct tcgaagccgc agttgccggc ggcgtccacc
     5281 cagtaatggc cgggaaggac ggcggtgatc tgctggaggg cggccacatc cacgggatgg
     5341 atctggcggc cgttgatgaa gacgccggtg gggttgcggc cggaggcgtc ggccttgagt
     5401 gggccgccga gctgcaggtt ggccggaagg aaaccgacgg tcgggccatc ctgggagccc
     5461 catgcgccgc acttcgcgtc gtaccagtaa gtgccggcca gcatggtgat gtggaactgt
     5521 gtttcgaatt tcgtgatctc ggcgggtgtg agcttcacgc cgttcacgac gatgtcgtcg
     5581 ctggctttct tgcgcgcgtc ggcatccttc ccggtggcgt tgaggccgac cgccatgtcg
     5641 gacgacgccc gctgaaacgt catcacccct tcctggccgg ccacggtcag cgcatcgccc
     5701 ttcagttcgt
//
LOCUS       DASVOD010000413         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_289436, whole genome shotgun sequence.
ACCESSION   DASVOD010000413 DASVOD010000000
VERSION     DASVOD010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289436"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF720_09470"
     CDS             <1..314
                     /locus_tag="VF720_09470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879626.1"
                     /translation="TSSGTPGGETGGAVLAANRPNPFNLSTRIHFVTTRHEPAASLVV
                     YDIAGRPVRHLFEGPLGAGAVEMSWDSRDDSGSPVPAGLYFCRLVTASGTQSRKMTVF
                     R"
     gene            329..>1976
                     /locus_tag="VF720_09475"
     CDS             329..>1976
                     /locus_tag="VF720_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7879627.1"
                     /translation="MIRIMKARWRSRATGLLLAAAALSAAGRNVQAETTTTDRFGIDR
                     YLNIRAAANPRLSPDDRYVAFLTNITGTNQVWRVPVEGGWPEQLTAYSEPISGFTYSP
                     VDNRIVFLRATGGSEQDQIYLLSADGADITPLVVEDGVTHYFGGWSWDGTKILYTSNL
                     RDKAIFDPYLLDLATMKSTRLAEETTSMFAAGFSPKDDMVLLRRQNTNIDIDIFMQPL
                     KGGERVLLTKHEGEANFSGGQWAKDQSAIFVTSNKDREFLAPARLEIPSAKLTWLDDT
                     KWDIEGVNFSFDGTHLARTDNVDGQSRLSLFDGGFGGRAMAAPELPPGLYSAPQFSRD
                     NELVVFAYQTSTRPNDIYTWRPATKTLNRITFSNLSGIPPESFVQPELVRFESFDGLS
                     VPAYIYMPTNMEPGTKPPCLVMMHGGPESQERPDFAGTWQYFLQRGYAILAPNVRGSS
                     GYGKTYLHLDDIEKREDSVKDMAAAVEWLKKQGRIDPDRVAVMGGSYGGYMVLAGLTL
                     YPELFQAGIDLVGIANFTTFLEKTSSYRRYWREVEYGFLEK"
BASE COUNT          349 a          670 c          637 g          320 t
ORIGIN      
        1 gcaccagctc cggcacaccg ggcggcgaaa ccggcggggc cgtcctggcc gccaaccggc
       61 ccaatccgtt caacctgagc acccggatcc acttcgtgac gacccgtcac gaaccggccg
      121 cctcgctcgt cgtgtacgac attgccggcc gtcccgtccg ccacctcttc gagggtccgc
      181 tcggcgccgg cgcggttgaa atgtcgtggg acagccgcga tgattccggc tcgccggtgc
      241 ccgccggcct ctacttctgc cgcctcgtga cggcgtccgg aacccagtcg cggaagatga
      301 ccgtcttccg ctgagcggag gagagtcgat gatccgaatc atgaaagccc ggtggcgttc
      361 gcgcgcgacc gggcttcttc ttgccgccgc ggcgctgtcg gcggcggggc gcaacgtcca
      421 ggccgagacg acgaccaccg atcgcttcgg catcgatcgt tacctgaaca tccgggccgc
      481 cgccaacccg cggctctcgc cggatgatcg ctacgtggca ttcctcacca acatcacggg
      541 cacgaaccag gtgtggcgcg tgccggtgga aggagggtgg cccgagcagc tcacggccta
      601 cagcgagccg atcagcgggt tcacctactc gccggtggac aaccgcatcg tcttcctgcg
      661 ggccacgggg gggagcgagc aggaccagat ctacctcctg tcggccgacg gggcggacat
      721 cacgccgctg gtcgtggaag acggggtgac gcactacttc ggtggctggt cgtgggacgg
      781 cacgaagatc ctgtacacct ccaacctgcg cgacaaggcg atcttcgatc cctacctgct
      841 cgacctcgcc accatgaagt cgacgcgcct ggccgaggag acgacctcga tgttcgccgc
      901 cggcttctcg ccgaaggatg acatggttct cctgcgtcgg cagaacacga acatcgacat
      961 cgacatcttc atgcagccgc tgaagggcgg cgagcgggtg ctcctcacga agcacgaggg
     1021 agaggccaac ttctccggag ggcagtgggc caaggaccag tcggccattt tcgtgacgag
     1081 caacaaggat cgcgagttcc tggcgccggc ccgtctcgag attccgtcgg cgaagctcac
     1141 ctggctcgac gacacgaagt gggacatcga gggggtcaac ttctcgttcg acggcacgca
     1201 cctggcccgc accgacaacg tggacggcca gtcgcgcctg tcgttgttcg acggcggttt
     1261 cggcggaagg gcgatggccg caccggaact cccccccggc ctgtacagcg cgccgcagtt
     1321 ttcgcgggac aacgagctgg tcgtctttgc ctatcagacg tcgacacggc cgaacgacat
     1381 ctacacctgg cggccggcca cgaagacgct gaaccggatc accttcagca acctgtcggg
     1441 catcccgccc gaatcgttcg tgcagccgga gctggtccgc ttcgagtcgt tcgacgggct
     1501 gtcggtgccg gcctacatct atatgccgac gaacatggag cccggcacga aaccgccctg
     1561 cctggtgatg atgcatggcg gaccggagag ccaggagcgc cccgacttcg ccggcacctg
     1621 gcagtacttc ctgcagcgcg gctacgccat cctggcgccg aacgtgcgcg gttcgtcggg
     1681 ttacggcaag acgtatctcc atctcgacga tatcgagaag cgcgaggact cggtgaagga
     1741 catggcggcc gccgtcgaat ggctcaagaa acaggggcgc atcgaccccg accgcgtggc
     1801 ggtgatgggg gggtcctacg gcgggtacat ggtgctggcc ggcctcacgc tctatcccga
     1861 actcttccag gccggcatcg acctggtcgg catcgccaac ttcaccacgt tcctcgaaaa
     1921 gacgagctcg taccggcgct actggcgcga ggtggagtac ggcttcctcg agaaac
//
LOCUS       DASVOD010000414         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_80797, whole genome shotgun sequence.
ACCESSION   DASVOD010000414 DASVOD010000000
VERSION     DASVOD010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80797"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1018)
                     /locus_tag="VF720_09480"
     CDS             complement(<1..1018)
                     /locus_tag="VF720_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX7879628.1"
                     /translation="MYPLLATPTRTLLYVAAWVPIALALAAVMWLVEPRPAWALAIVV
                     GPLCLVYASATTSNWWILRGEKPENLLSVRLLTRMVTAAIVTAMLWAVAANLLYTFLD
                     PRLPSFIDKAVRRKDLILIFVAGVGIYWLSSALHLAFFISIKAVEAERRALESQVSAR
                     EAELKALRSQLNPHFLFNSLNSIMSLVGRSPEEARRMCQGLGDFLRRTLNLGSRERVS
                     LAEELDLVRHYLEIEAVRFGARLTYELDVPADLNAIQVPPLLLQPLIENAVKHGVASR
                     LDGGRIHLTARRESDRLYLSVTNPTDEEAAPVDGEGMGLDNVRRRLKATAAGRGTLEI
                     RRRPG"
     gene            complement(1022..>1704)
                     /locus_tag="VF720_09485"
     CDS             complement(1022..>1704)
                     /locus_tag="VF720_09485"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HEX7879629.1"
                     /translation="PFGQLIIGGTILFVGFLWTLDAMDILDTEEIIQYWPLGLVLLGA
                     LKLFGIGMRRSLVGGLFLTIIGVLIIGNETGALYLSMRLFWPIVIMALGAVLVARSFG
                     WMGGRIGAVGPGEGSTAVMGGVLHREPAQLFQGGSLTAIMGGVDYDLSQATGIEPDAA
                     VDVFAFWGGIDMAVPPNWRIDMRVTALLGGITDSRSPSPAGLTGPTLVVRGIVMMGGL
                     DIKDRVDR"
BASE COUNT          303 a          575 c          589 g          237 t
ORIGIN      
        1 cgcccgggcg acgccggatt tcgagcgtgc cgcgcccggc cgccgtggcc ttgaggcggc
       61 ggcggacgtt gtcgagcccc atcccctccc catccaccgg cgcggcctcc tcgtcggtgg
      121 gattggtgac cgacaggtag agccgatcgc tttcccgccg ggcggtcagg tggattctcc
      181 caccgtccag acgggaggcc acgccgtgct tcaccgcatt ctcgatgagg ggctggagga
      241 ggagtggtgg aacctgtatg gcattcaggt ccgcggggac gtcgagctca tatgtgagcc
      301 gcgccccgaa ccgcaccgcc tcgatctcga ggtaatgccg taccagatcg agctcctcgg
      361 ccagcgacac ccgctcgcgc gaccccaggt tgagcgtgcg acgtaggaag tcgcccagcc
      421 cctggcacat ccgccgggcc tcctcggggc ttcgccccac cagtgacatg atcgagttca
      481 ggctgttgaa caggaaatgc gggttgagct gcgagcggag cgccttgagc tcggcctccc
      541 gcgcgctcac ctgcgattcg agggcccgtc gctcggcctc gacggccttg atcgagatga
      601 agaacgccag gtggagcgcc gacgacaacc agtagatgcc gacgccggcc acgaagatga
      661 ggatcaggtc cttgcgacgg acggccttgt cgatgaacga agggaggcgc ggatcgagga
      721 acgtgtagag gagattcgcc gccaccgccc acagcatggc cgtcacgatc gccgcggtca
      781 ccatccgcgt gaggagccgc accgagagga ggttctccgg cttctccccc cgcaggatcc
      841 accagttgga cgtggtggcc gaggcgtata ccaggcaaag ggggccgacc acgatggcca
      901 gcgcccaggc cggccgcggc tccaccagcc acatgaccgc cgccagcgcc agggcgatgg
      961 gaacccacgc cgccacgtac aggagcgtgc gtgtcggcgt ggcgaggagg gggtacatga
     1021 cctagcggtc gacccgatcc ttgatgtcga ggccgcccat catcacgatg ccgcgcacga
     1081 ccagcgtggg gccggtcagg ccggccggcg agggactgcg gctgtcggtg atcccgccga
     1141 ggagcgcggt gacgcgcatg tcgatgcgcc agttcggcgg aacggccatg tcgatgccgc
     1201 cccagaaggc aaagacatcc accgcggcgt cgggctcgat gccggtggcc tgcgagaggt
     1261 cgtaatccac gccacccatg atcgcggtca acgagcctcc ctggaagagc tgggccggtt
     1321 cccggtgcag gacgcccccc atgacggcag tcgagccttc acccgggccc acggcgccga
     1381 ttcgtccccc catccagccg aacgagcggg ccaccagcac cgcgccgagc gccatgatca
     1441 cgatgggcca gaagaggcgc atgctcaggt agagcgcgcc ggtctcgttg ccgatgatga
     1501 gcacgccgat gatggtgagg aagaggcccc cgaccaggct gcgccgcatc ccgatgccga
     1561 agagcttgag ggcgccgaga aggacgaggc cgaggggcca gtactggatg atctcctcgg
     1621 tgtcgaggat gtccatggca tccagcgtcc agaggaaccc cacgaagagg atcgtgccgc
     1681 cgatgatcag ctgcccgaag ggcg
//
LOCUS       DASVOD010000415         8665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_236659, whole genome shotgun sequence.
ACCESSION   DASVOD010000415 DASVOD010000000
VERSION     DASVOD010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236659"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VF720_09490"
     CDS             <1..902
                     /locus_tag="VF720_09490"
                     /inference="COORDINATES: protein motif:HMM:NF015451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7879630.1"
                     /translation="AWRLTTPAGATIARAVILATGGLSFPKTGSDGSGYRLATALGHT
                     LSPRTPALTPLVVSGMALVPLVGLTLPAALTLHVNGQRAVTCAGSLLVTHFGLSGPAA
                     LDLSRHWLRAPAGATRRVTIHWRPGPARPLEEGGGLLDPAAAEKPGGLPDPGKLEAGW
                     QRDAAGEAPTIGQWLGRDLPARLVRTLLERAEVEPATRLTQVSRERRRLLLELVAAFP
                     VDVVDTRGYAKAEVTAGGVPLDEIDVRRMMSRRAPGLFLAGEIVDVDGRLGGYNFQWA
                     WSSGRLAGLSAAAWVRDGSRDPG"
     gene            complement(874..1533)
                     /locus_tag="VF720_09495"
     CDS             complement(874..1533)
                     /locus_tag="VF720_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879631.1"
                     /translation="MNMAATSRKLARTALLLAGAMTLVVAPVSMCRAAEPHPFEITPF
                     GGYRFGGRLEVLLGRLVMSDAPVFGGVLGYRVAPDALLEFTYVQENTDFTFEPTASGD
                     NVFVTDLAIRSFYIGGVYEHGLSQVRPYAGMSIGMSRFDPELESAAAETRFSFHFMGG
                     LRTDFSQAVGLKWGVRGIFSTPPDDGSVILCTDDETCYTQTGNSLTSQFDFTLGLVIR
                     P"
     gene            complement(1537..2067)
                     /locus_tag="VF720_09500"
     CDS             complement(1537..2067)
                     /locus_tag="VF720_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7879632.1"
                     /translation="MKRQLALAASLLLTAALFAGCSSKKDEGPMAETTTQTPTPTGSQ
                     ILDPHPTSWDRSAMPNPAPSTRPMYRVNEISFAQGTTKSGPEGDGVCREVASSLKAKG
                     VKKVLLIGFTDRSESDGNLAMKRAESVRSCLVSHEMRNDQFEIASYGSSMAKGDKQTP
                     TQMEQDRRVEIWVLSE"
     gene            complement(2157..2936)
                     /locus_tag="VF720_09505"
     CDS             complement(2157..2936)
                     /locus_tag="VF720_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879633.1"
                     /translation="MISLLEAAWWVNRFERPDLDTAPYETAVDAMAARLRDAATAEGG
                     AVELAAGLRMVMHVTHGFRGDEQDYHDPDNSYLDRVMDRRRGIPISLSILAVEIVRRA
                     GHVAEGIAFPGHFLVRLEAGGTRCLVDPFGGCLVVTDADLRGLLARVMGAGSMVTAAM
                     TAPAPPAAVARRMLNNLKGNYLRSGDLTRALRTLDAILTLAPDDPVERRDRGLVREAL
                     EHDGGALDDFEAFLDALPDADDANAIRARLAPLRIRLSQWN"
     gene            complement(2933..4018)
                     /locus_tag="VF720_09510"
     CDS             complement(2933..4018)
                     /locus_tag="VF720_09510"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEX7879634.1"
                     /translation="MFARVPALAFATSAPGFTPVLEPRTWSFPRDHGSHPGFQTEWWY
                     WTGHLEARGHRYGYEVVFFRRVPPRPTDLGPGYEGWQAAEIHPAHVALTDIDGKSFHH
                     EQRLFRTIGGIAAADTARLDVRCGDWTARQEGESFVVSAGMDDWHLDLTMTSTKPPAL
                     HGREGISTKGPVAGQASYYYSLTRLETRGTLTRGDRTEPVTGLSWMDHEFFSSDLDTT
                     LAGWDWFSLQLDDGTELMLYRLRGRPGTDRDWLSGSFIDRAGAVQPLRREAITIVPQR
                     TWTSPTTGAVYPLGWQVTIAPLGFTLSLSPDRDDQELDTRGSTGVVYWEGSVSVTGTR
                     EGKVLTGVGYAELTGYDGRTPMRGATP"
     gene            complement(4081..6636)
                     /locus_tag="VF720_09515"
     CDS             complement(4081..6636)
                     /locus_tag="VF720_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX7879635.1"
                     /translation="MIRLLRYTAWRRLRSDRLRAVITLLGIALGVAVVLAIELANRAT
                     TRSVETMVENIAGKARLAVRGDEAGIPESLLARVMDHPGVAAALPVIEARLDHVESGR
                     ALVVLGVDLLADAAPRGYGMTLKEPLELVSRPDRVIVTESFAAAESLSVGSPMTLMTP
                     LGPRIVRVGGTLPGGDMADAVGGRLLLIDIRAAQFLLARPGRLDRIDLVPTNEWQGRS
                     MASTSALSALAADLALRLPPGLRVEPPAARAAEAVELLASFQVNLRMVSLVALFVGLF
                     LVYNTMSIAVVQRRRETGILRAIGVTKGRITLLITLEGFAWGVAGSALGLVLGVILAR
                     GALLAVSSTLGRAYLPVSATEVPLSWPPMVAAALLGIGVSTVAAWLPGREGAAQPPAI
                     TIRSLPFQDGGRRRLGMLVALAVVLLAGAALMSRLGPVNGVAWFGYLAGFGIVFGVAA
                     LSPVVCLQFSRVVRPLYAARFGVTGALAADNLGRSLGRTGLSVSALMTGLSLVVCVAT
                     MIHSFKHSIVVWVANTVRADLLVGAGTGDSPGTNLPLPRSMGDSLAAIPGVARVNSFR
                     MIRAHVGERTVALGSLDLAEWTATNPLVARERRAEPPDAASAIVAENFADRFGVAVGD
                     SITLDAPSGPLRLAVTAVILDYSSDRGALFIDRNTYRSAWGDDRVDNFDLYLEPGASS
                     ATVAATIENRFGKSTGLFLVENRQMRQRILASIDNTFAIVYALEAIALAIAVLGIVNT
                     LVASILDRRRELGLLRAVGATRRQVGRLFILEAASMGLLGILLGLVAGVLLSWLLVNV
                     IQFQSTGWRFLYRFPFVMVAATSLVTLVAAALAGWYPARLGARTWGAGALQYE"
     gene            complement(6650..7351)
                     /locus_tag="VF720_09520"
     CDS             complement(6650..7351)
                     /locus_tag="VF720_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005208511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7879636.1"
                     /translation="MLQLDRIRKVYPRGAAGVVALDDISLHVDRGEFLSVMGPSGSGK
                     STLLNLVGCLDRPSSGLLTIDGVETTELDDDALTEVRRRRVAIIFQFYNLLPTLTAVE
                     NVALPRLLDGAREGDTTRRAEKLLERVGLAHRLRFRPDELSGGEMQRVAIARALMSEA
                     PLLLADEPTGNLDSATAGSVMELLVETVREVGLTLVLVTHDAGVAARADRHVVIRDGR
                     LVADDRRPAGSAASA"
     gene            complement(7527..8459)
                     /locus_tag="VF720_09525"
     CDS             complement(7527..8459)
                     /locus_tag="VF720_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879637.1"
                     /translation="MSFVIHARLFALATTLALMILPPASPSEAALAPDADRLLLADSL
                     FVAGRWADAAPIYRELTMAAPEVARHWARYGTCLHNLGIFDDAVQAMTKAESIGHNPT
                     VMFNLACALARSGREDEALNWLSKASQGGFTNLAALESDDDLASLRSHPRWKEVWTDF
                     DRQARPCSYDDRYRQLDFWLGDWDVKTAQGQPAGTSQVEMILNQCIIFENWTGFSGMS
                     GKSFNFIEPGTGVWRQTWVDDKGDAHEYRGEFVDGEMRYRRESKGADGRVVLNKMTFF
                     NLAPGHVRQLGEHSDDDGKSWTVDYDLHYFRRKA"
BASE COUNT         1422 a         2889 c         3079 g         1275 t
ORIGIN      
        1 ccgcctggcg gctgaccacg ccggccggcg ccaccatcgc ccgggcggtc atcctcgcta
       61 ccggtggact ttcgtttccg aagaccggca gcgacggctc gggatatcgg ttggccacgg
      121 cactcggtca cacgctgtcg ccgcggaccc ccgcgttgac gcccctcgtg gtgagcggca
      181 tggcgctggt gccgctggtc ggcctcaccc tgcccgcggc gctgacgctt catgtgaatg
      241 gacagcgcgc ggtcacctgc gccggctcgc ttctcgtcac gcacttcgga ttgtccggtc
      301 cggcggcgct cgacctgagc cgccactggc tgcgagcgcc ggccggcgcc acacgccgcg
      361 tcacgattca ctggcgtccc gggcccgcca ggccgctgga agaagggggc gggcttctcg
      421 atcccgcggc cgccgagaaa ccgggaggtc tgcccgaccc gggaaaactc gaggccggat
      481 ggcaacgcga tgccgccggc gaagcaccca ccatcggcca gtggctgggg cgtgacctgc
      541 cggcacggct cgtccgcacc ctcctggagc gggcggaggt cgaacccgcg acgcgcctca
      601 cccaggtatc cagggagcgc cgccggcttc tgctcgaact ggttgccgcg tttccggtcg
      661 acgtcgtcga cacgcgggga tacgccaagg ccgaagtgac ggcgggcggg gtgcccctgg
      721 atgagatcga cgtgcgacgg atgatgtcgc ggcgcgcgcc cgggctcttc ctggccggcg
      781 agattgtcga cgtggacggc cggctcgggg gttacaactt ccagtgggcg tggagcagcg
      841 gacggctggc cggcctctcg gcggcagcct gggtcaggga cggatcacga gacccagggt
      901 gaaatcgaac tggctggtga gcgagttgcc cgtctgggtg tagcaggttt cgtcgtccgt
      961 gcagaggatc acggagccgt cgtcgggggg cgttgaaaag atcccgcgga caccccactt
     1021 gagccccacc gcctgcgaga aatcggtgcg caggcccccc atgaagtgaa acgaaaagcg
     1081 cgtttcggcc gcggcactct cgagttcggg gtcgaagcgc gacatcccga ttgacatgcc
     1141 ggcgtagggg cgcacctggc tcaggccgtg ctcgtacacg ccgccgatgt agaagctccg
     1201 gatggcgagg tcggtgacga agacgttgtc gccgctggcc gtcggctcga aggtgaagtc
     1261 ggtgttttcc tggacatagg tgaattcgag gagggcgtcg ggtgctacgc ggtaaccaag
     1321 cacgccgccg aacaccgggg cgtcactcat cacgagacga cccaggagca cttcgagacg
     1381 tccgccgaag cggtagccgc cgaacggggt gatttcgaac gggtggggtt ccgcggcacg
     1441 acacatggaa acgggggcga cgaccagcgt catcgccccc gcgagcagga gagctgtccg
     1501 ggcgagcttt cgcgaagtgg cggccatgtt cacgcgttac tccgaaagga cccagatttc
     1561 gacgcggcga tcctgctcca tctgcgttgg ggtctgcttg tcgcccttgg ccatgctcga
     1621 tccatagctg gcgatctcga actggtcatt gcgcatttcg tgggacacca ggcagctgcg
     1681 aacgctctcg gcgcgcttca tcgccaggtt gccgtccgat tccgacctgt cggtgaagcc
     1741 gatgaggaga accttcttca ctcccttggc cttcagcgac gacgccacct cgcggcaaac
     1801 gccatcaccc tcgggaccgc tcttcgtggt cccctgggcg aagctgattt cgtttacccg
     1861 atacatgggc cgggtggacg gcgccgggtt gggcatggcc gaccggtccc agctggtggg
     1921 gtgcgggtcg aggatctggg acccggtggg ggtgggggtc tgggtagtgg tctccgccat
     1981 cggaccctca tccttcttgc tggaacagcc ggcaaacagg gctgcggtga gcaacagcga
     2041 agcggccaga gcgagctgcc gtttcatgtg gaacctcctg agttgagtca aggggcggcg
     2101 cagatccaca tccaatctag cagtcccggt atcgacaggg aaaggcgcca gtcccatcaa
     2161 ttccactgcg agagtcggat cctgaggggg gccaggcggg cccggatggc gttggcgtcg
     2221 tcggcatcgg ggagggcatc gaggaaggcc tcgaagtcgt ccagggcgcc gccgtcgtgc
     2281 tcgagggctt cgcgcacgag gccccggtct cgccgttcca ccggatcgtc gggtgcaagc
     2341 gtgagaattg cgtcgagtgt ccggagggcg cgggtgagat cgcccgaccg gaggtagttc
     2401 cccttgagat tgttcaacat ccgccgggcc acggctgccg ggggggccgg cgcggtcatt
     2461 gccgccgtta ccatggagcc tgcccccatg acgcgggcca ggagcccgcg gagatcggca
     2521 tcggtgacca ccaggcaccc gccgaacggg tcgaccaggc accgcgtgcc gccggcctcg
     2581 agccggacca ggaagtgccc ggggaaggcg attccctccg cgacgtgacc ggcgcgacgg
     2641 acgatttcga ctgcgaggat ggaaagcgat atcgggatgc cccggcggcg atccatcacc
     2701 cgatcgagat aggagttgtc gggatcgtgg taatcctgtt catctccccg gaaaccgtgc
     2761 gtcacgtgca tgaccattcg gagaccggcg gccagctcga ccgcgccgcc ctccgcggtc
     2821 gccgcgtcgc ggagtcgggc tgccatcgcg tcgacggccg tctcgtaggg ggccgtgtcg
     2881 agatctggcc gctcgaaccg gtttacccac caggccgcct cgagaaggga gatcacggcg
     2941 tggcgccgcg catcggcgtg cgtccgtcgt agccggtcag ttccgcgtag cccactcccg
     3001 tgagcacctt cccctcccgc gtgccggtga ccgacacgct tccttcccag tacaccacgc
     3061 ccgtcgatcc ccgcgtgtcg agctcctggt cgtcccgatc gggggagagg gaaagggtga
     3121 agccgagcgg cgcgatggtg acctgccacc ccagggggta aacggcgccg gtcgtcgggc
     3181 ttgtccaggt gcgttgcggc acgatggtga tcgcctcccg ccgcaggggc tgcacggcgc
     3241 ccgcccgatc gatgaacgac ccggaaagcc agtcacgatc cgtcccgggc ctgccgcgca
     3301 gtcggtagag catgagctcc gtgccgtcgt ccagttgaag gctgaaccag tcccagccgg
     3361 ccagcgtcgt gtcgaggtca ctcgagaaaa attcgtgatc catccacgag aggccggtca
     3421 ccggctcggt ccggtcgcca cgggtcagcg tgccccgcgt ctcgagacgc gtcaacgagt
     3481 agtagtacga ggcctggccg gccaccggtc ccttggtcga aatcccctcg cggccgtgga
     3541 gcgccggggg tttcgtggag gtcatcgtga ggtcgaggtg ccagtcgtcc atccccgcgc
     3601 tcacgacgaa gctttccccc tcctggcggg ccgtccagtc gccgcagcgc acgtcgagcc
     3661 gcgccgtgtc ggcggcggcg atgccgccga tcgtccggaa gagacgttgc tcatgatgga
     3721 atgatttccc atcgatgtcg gtgagcgcca cgtgggccgg gtggatctcg gcggcctgcc
     3781 aaccctcgta gccggggccg aggtcggttg gccgcggcgg cacgcggcgg aagaagacca
     3841 cctcgtatcc gtagcgatgc cccctggcct cgagatgccc ggtccagtac caccactcgg
     3901 tctggaatcc gggatgcgac ccatggtctc gcggaaacga ccaggtgcgc ggttcgagaa
     3961 cgggggtgaa gccgggggcg gacgttgcga acgcgagtgc cggcacccgc gcgaacacgt
     4021 atgccaacgc caacgccgac aagagagcca gcggagcccg tgaatgccgc gccacgcgtc
     4081 tcactcgtac tggagcgcac ccgcgcccca cgtcctggcg ccgagccggg ccgggtacca
     4141 ccccgccagc gccgcggcga ccagcgtgac caggctcgtg gccgccacca tcacgaacgg
     4201 gaagcggtag aggaagcgcc agccggtgga ctggaactgg atgacattca ccaggagcca
     4261 cgagaggagc acgcccgcca cgaggccgag caggatgccg aggaggccca tcgaggcggc
     4321 ctcgaggatg aagaggcgtc ccacctggcg gcgggtggcg cccaccgcgc ggaggaggcc
     4381 gagctcccgc cgccggtcga ggatggaggc caccagcgtg ttcacgatgc cgaggacggc
     4441 gatggccagc gcgatggcct cgagcgcgta gacgatcgcg aaggtgttgt cgatgctggc
     4501 gaggatgcgc tggcgcatct gtcgattctc cacgaggaag aggccggtgg actttccgaa
     4561 gcggttttcg atggtggccg ccaccgtggc gctcgaggcc ccgggctcga gataaagatc
     4621 gaagttgtcg acccggtcat ctccccacgc cgagcgatac gtgttgcggt cgatgaagag
     4681 cgcgccgcga tcgctggaat aatcgaggat gacggccgtc accgccagtc ggagaggccc
     4741 tgagggggca tcgagggtga tggagtcccc caccgccacg ccgaaccggt cggcgaaatt
     4801 ctcggccacg atggccgagg cggcgtcggg gggttcggcc cggcgttcgc gcgccacgag
     4861 cggattggtg gcggtccact ccgccaggtc gaggctcccg agcgccacgg tccgttcccc
     4921 gacgtgggcc cggatcatcc ggaaactgtt cacgcgcgcc acccccggaa tggccgccag
     4981 ggaatcgccc atcgagcggg gaagggggag gttcgtgccc ggtgagtcgc cggtgccggc
     5041 accgacgagg agatcggccc ggaccgtgtt cgcaacccag acgacaatcg agtgcttgaa
     5101 cgagtggatc atggtggcca cgcagaccac cagcgagaga ccggtcatga gcgccgatac
     5161 ggaaagaccg gtgcgcccga gcgagcgccc caggttgtcg gcggccaggg cgccggtgac
     5221 gccgaagcgc gcggcgtaca acggacgaac cacccggctg aactgaaggc acacgaccgg
     5281 cgacagcgcc gccacgccga agacgatccc gaaaccggcc aggtaaccga accaggccac
     5341 gccgttcacc ggtccgagtc gcgacatgag tgctgcgccg gcgagcaaca ccaccgccag
     5401 cgccaccagc atgccgaggc gccgtcggcc gccatcctgg aaggggagcg aacggatggt
     5461 gatggccggc ggctgcgcgg ccccctccct tcccggcagc caggccgcca cggtcgaaac
     5521 cccgataccg aggagcgccg cggcgaccat gggcggccat gacagcggca cctcggtggc
     5581 cgacaccggc aggtaggcgc ggccgagcgt ggaactcacg gcgaggagcg cgccgcgcgc
     5641 caggatcacg ccgagcacga ggccgagcgc cgagccggcc actccccacg cgaacccctc
     5701 gagcgtgatg aggagcgtga tgcgcccctt ggtgacaccg atcgcacgca ggatccccgt
     5761 ctcgcggcgg cgctgcacca ccgcgatcga catcgtgttg tacacgagga agagcccgac
     5821 gaagagcgcc accaggctca ccatgcgcag gttgacctga aacgaggcca ggagctcgac
     5881 ggcctcggct gcgcgcgccg cgggcggttc cacccgaagg ccgggtggaa ggcggagggc
     5941 caggtcggcg gccagtgcgg agagtgcgct ggtgctcgcc atcgaacgcc cttgccactc
     6001 gttcgtcggc accagatcga tgcgatcgag ccgccccggc cgcgccagga ggaattgggc
     6061 ggcgcggatg tcgatgagga gaaggcggcc gccgaccgcg tcggccatgt cgccgcccgg
     6121 gagggtgccc ccgacgcgca cgatgcgcgg cccgaggggc gtcatcaacg tcatgggcga
     6181 tcccaccgag aggctctcgg cggcggcgaa cgactcggtc acgatcaccc ggtcgggtcg
     6241 tgacaccagc tcgagcggtt ccttgagcgt catgccgtag ccgcggggag cggcgtcggc
     6301 cagcaggtcc acgccgagga ccaccagcgc tcgcccgctc tccacatgat cgagccgcgc
     6361 ctcgatcacg gggagcgccg ccgcaacgcc gggatgatcc atcacccgcg cgaggagcga
     6421 ctccgggatt cccgcctcgt ccccgcggac cgccagccgg gcttttcccg cgatgttctc
     6481 gaccatcgtc tcgaccgagc gggtggtggc gcgattggcc agctcgatgg ccagcaccac
     6541 cgccacgccg agcgcgatgc cgagaagcgt gatgacggcg cgcagccggt ccgaacggag
     6601 ccgtcgccag gcggtgtagc ggaggagccg gatcacgcgc ggcccgggtt cacgcggagg
     6661 ctgcggatcc ggccgggcgc cggtcgtccg ccaccaggcg tccgtcccgg atcaccacgt
     6721 gccggtcggc gcgggccgcc acaccggcat catgcgtgac gagcaccagt gtgaggccca
     6781 cttcccggac cgtctccacc agcaactcca tcaccgagcc ggccgttgcg gaatcgagat
     6841 ttccggtcgg ctcatcggcc aacaggagcg gagcctcgga catcaacgcc cgggcgattg
     6901 ccacgcgctg catctctccc cccgagagct cgtccggccg aaaccggagc cggtgggcga
     6961 ggccgacgcg ttccagcagc ttctcggccc gacgggtggt gtccccctcg cgagcgccgt
     7021 cgagaagccg cgggagcgcc acgttctcga ccgcggtgag cgtcggcagc aggttgtaga
     7081 actggaagat gatggccacc cgtcggcgac gcacttcggt gagcgcgtcg tcgtcgagct
     7141 cggtcgtctc gacgccgtcg atcgtgagga ggccggacga ggggcgatcg aggcacccga
     7201 ccaggttgag caacgtgctc ttcccggagc cggaagggcc catgacgctc aggaactcac
     7261 cgcggtcaac gtggagcgag atgtcgtcca gcgccacgac gcccgcggcc ccgcggggat
     7321 agacctttcg aatgcgatcg agctgcagca tgggacctcc ggaccggacg gacgagctta
     7381 gactgtccgg gacggggatc gatgcgcgaa atcccagggt ggggaggggc ggtcggggga
     7441 ttgtgtcccc gccggccgcc cccgggctca gggcccgggg gcggcccggc actcacggat
     7501 gcctcccgtg gggtgacccg agggggtcag gccttgcgcc ggaagtagtg gaggtcgtag
     7561 tcgaccgtcc acgacttgcc gtcgtcatcg gagtgttccc ccagctgccg gacgtgtccg
     7621 ggcgccaggt tgaagaaggt catcttgttc aggacgacgc gaccgtcggc gcccttgctc
     7681 tcccggcggt agcgcatctc gccgtcgacg aattcgccgc ggtattcgtg ggcatccccc
     7741 ttgtcgtcca cccaggtctg gcgccacacg ccggtgcccg gctcgatgaa attgaagctc
     7801 ttgccgctca tcccggaaaa gccggtccaa ttctcgaaga tgatgcactg gttcaggatc
     7861 atctccacct ggctggtgcc ggccggctgc ccctgcgccg tcttcacgtc ccagtcgccg
     7921 agccagaagt cgagctgccg gtatcgatcg tcgtacgagc agggccgggc ctgccgatcg
     7981 aagtcggtcc agacctcctt ccaccgcggg tgcgagcgga gggaggccag gtcgtcgtcc
     8041 gattcgaggg ccgcaaggtt ggtgaagccc ccctgcgatg ccttcgacag ccagttgagc
     8101 gcctcgtcct cgcggccgct ccgcgcgagg gcgcaggcca ggttgaacat gacggtcggg
     8161 ttgtggccga tcgattccgc tttcgtcatg gcctggacgg cgtcatcgaa aatgccgagg
     8221 ttgtgcaggc aggtgccata gcgcgcccag tgccgggcca cttccggcgc cgccatcgtg
     8281 agttcgcggt agatcggcgc cgcatcggcc catcggccgg ccacaaagag gctgtcggcc
     8341 aggaggagcc gatcggcatc gggcgcgagg gcggcttcgg acggtgaggc cggcggcagg
     8401 atcatgagtg cgagagtggt ggccagggca aacagccggg cgtgaatcac gaacgacatg
     8461 ggtctctcca gtaaggcagc tggttgggcc cgcaccggaa tatagatgag cccgacagga
     8521 accggacggg ggcgctctcc gttacggggt gtttttacaa cggtttacat gatttacagt
     8581 gcgcggatgg aacgtttggg atgagccgcg gccagggcgt gatgaacggg ggagtccggc
     8641 tggcggcctg cctgctcctg ggcct
//
LOCUS       DASVOD010000416         5436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_29942, whole genome shotgun sequence.
ACCESSION   DASVOD010000416 DASVOD010000000
VERSION     DASVOD010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29942"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VF720_09530"
     CDS             <1..201
                     /locus_tag="VF720_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879638.1"
                     /translation="ESKAAVEVATGSIDMAAIERERTARKLKAAGILQQFEVEMGLAK
                     PTSVGESAAPTVGPRIVEKQGP"
     gene            211..1110
                     /locus_tag="VF720_09535"
     CDS             211..1110
                     /locus_tag="VF720_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879639.1"
                     /translation="MASPENDRDEESAGSILRELILNQYQGILLAGSTLASVLTLNPM
                     PLLVWLGSELVLLPILDSGPLRRMVARKRREAARKDSTRRRQQVIKSFGPEYARRFAS
                     LENLCRQIEGNYQGLHGISRAYLSEQRGKLDLILDSCLQRMTALQRYERTLNRKAPER
                     IEEEIAGLEQELADPQLNERARAAIEKNLELKKRLLQSYVDAEGTMKALSTELDSMAS
                     LLEVLLQSSISMRDPAAISDELDTIVRQSEDSGRVVREMETLLREGVPEWDSGLPDLP
                     ESTFTQRAGDAVDKAARQRMPKR"
     gene            1107..2858
                     /locus_tag="VF720_09540"
     CDS             1107..2858
                     /locus_tag="VF720_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7879640.1"
                     /translation="MSHPIEAWPDWARRLAHAIRARLGNSFILHGNTHDLMSAPGAMA
                     PGVPATRFVSLPEFLAEQVFGSREVVIEYQRANGAIFHTRESHRLFSETLSTIDTVHG
                     TDYARAIPRDPATFCSLLDSFLKQMLLKETKPGVAVLFPWAETLLPESSGEAGAEDRA
                     VRVFFQKWATDPGFLAANVTFVLMTENLADINTRLVRSPQTVELEVKRPNEQERLDFL
                     RWSRDDAWFAGRSDLPPERLAQFTSGLTRIQLRQILASVDEQGDRLDARLLREQKKAV
                     IEAECFGLLEYVESPHSLDMVSGHEGVKQRLRRAAKAIGAGRLRGVPMGYLVAGPVGS
                     AKTFLVTCFTGEIGFPCVKFLNFRSQWQGVTEGNLEKILKLLHAMWPVGVIIDEADAF
                     LGDRNQQGDSGTSNRVFAQFATFMANTDYRGKIVWFLITSRPDLLPIDLKRQGRAEEH
                     LALFYPQHDAEYDALLRIMLRKSGLTSPVTSIREVIDDPAGLSGADIEAILARAALVA
                     DAEGVGDGRSLTADVLKQVFADFIPTSNALERELQVLVAVQECTSREILPEVYRSMDR
                     ADLAARINELRVLLNEA"
     gene            2909..4561
                     /locus_tag="VF720_09545"
     CDS             2909..4561
                     /locus_tag="VF720_09545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015334348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7879641.1"
                     /translation="MARTPLFDSLTQALRIAAFAARAKSNVPPLDELVDMAGSRRRFL
                     KQAAASAAAAAFYGCAQPKRDARQVAPPPQTATAANPRIVIVGGGMAGLNTAYKLQKA
                     GLTATIYEGSDRTGGRMFTAKDLLGTGITTELGGEFIDSGHAEMLALMQEFGLESIDT
                     HAPEVTNLKKETYFVNGRHYSIAQAAQEFVPLAARIQEHYDSMGEVVDYQTDGGGGTF
                     DRMSIAEYLDSIGSKGWLREVLDVAYVTEYGLEAAEQSSLNFLFLIGTGDLTDASTIE
                     LLGESDERFKVKGGNQRVVDELARRLDPQIQRRHRLEAIKPRGAGYTLTFQVDGAPRD
                     VEADIVVLAMPFTLLREVDIQVPLPDVKTKAIRELGYGTNAKVLVGFKDRPWIRKGYS
                     GATYSDEMFQLCWDNSFLQAGTEGGITLYSGGKLGLDAGNGTAEEAAARLMPGFERTY
                     PGASARRNGRTSRFHWPTFPWTKASYACYKPGQWTTIAGAEGVPVGNLFFAGEHCSYD
                     FQGYMNGAAQSGLDTAKAVMGLLSGSPVDSGMLFEREAALVG"
     gene            4574..>5436
                     /locus_tag="VF720_09550"
     CDS             4574..>5436
                     /locus_tag="VF720_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7879642.1"
                     /translation="MKVRGSESGAVHLTVAGRLLIMVVGLAVLGYVAYMYRDRLPFKP
                     GAPKSNTSTTSRTDDAHPSGGAAPAASQGGVMARIRNAGVVRVGMEPDAAPLHFVNDR
                     KQEDGFDFRLAGVIADALKSGTRVQVVEADYEDLPGQLTAGNIDLIMAGYVPDPSIDG
                     VDWSNGYLDFGLCMIVAEGNVSQFRSPADLAGKRVAIYDDPAAERWVESNIPNARISK
                     FSGDSGWFEAVEDDKADALIYDYPFAAEEIKEHPRTVIVRYNLNASKYAVGIPKGNYD
                     LVYEVNQAIEN"
BASE COUNT          952 a         1843 c         1798 g          843 t
ORIGIN      
        1 gagtcgaagg ccgccgtcga ggtggccacg ggttccatcg acatggccgc aatcgaacgg
       61 gagcggacgg cgcgcaagct gaaggcggcc ggcatcctcc agcagttcga ggtggagatg
      121 gggctggcga aacccacgtc ggtgggcgaa tcggccgcgc cgacggtcgg gccgcgcatc
      181 gtcgagaagc aggggccgta gaccgaagcg atggcgtcgc ccgagaacga ccgggacgag
      241 gagtcggcgg gctcgatcct gcgcgagctg atcctcaacc agtaccaggg gatcctgctg
      301 gccggctcga cactggcctc ggtgctcacc ctgaacccga tgccgctcct ggtctggctc
      361 gggtcggagc tcgtgctcct gccgatcctc gactcggggc cgctccggcg catggtggcc
      421 cgcaagcgtc gcgaggccgc gcggaaggat tccacccggc gccggcagca ggtcatcaag
      481 tcgttcggcc cggagtacgc ccggcgattc gcctcgcttg aaaatctgtg caggcagatc
      541 gaagggaact accagggact gcacggcatc tcgcgcgcct atctctcgga gcagcgcggc
      601 aagctcgacc tgatcctcga ctcgtgcctc cagcggatga cggcgctcca gcgctacgag
      661 cgcacgctca accgcaaggc gccggagcgc atcgaggagg agatcgcggg cctcgagcag
      721 gaactggccg atccccagct gaacgaacgt gcgcgggcgg ccatcgagaa gaacctcgag
      781 ctgaagaagc gcctcctgca gtcgtatgtc gatgccgaag ggacgatgaa ggcgctttcc
      841 accgagctcg actcgatggc ctcgctgctc gaggtgctgc tgcagtcgtc catctcgatg
      901 cgggatccgg cggcgatttc cgacgagctc gacacgatcg tgcgccagtc cgaggattcc
      961 ggtcgcgtgg tgcgggagat ggagacgctc cttcgcgaag gagtgcccga atgggattcg
     1021 ggcctgccgg atcttcccga atcgaccttc acgcagcggg ccggcgatgc ggtggacaag
     1081 gcggcccgcc aacggatgcc gaaacgatga gccatccgat cgaagcctgg cccgactggg
     1141 cccgccgcct ggcccacgcc atccgggcgc ggttgggaaa ttccttcatc ctgcacggga
     1201 acacgcacga tctcatgtcg gcgccgggcg ccatggcgcc cggtgtcccg gcaacccggt
     1261 tcgtgtccct gcccgagttc ctggccgagc aggtcttcgg cagtcgcgaa gtcgtcatcg
     1321 aataccagcg cgccaacggt gcgatcttcc acacccgtga gtcgcaccgc ctcttcagcg
     1381 agacattgag cacgatcgac accgtgcacg gcaccgacta cgcccgcgcc atcccgcgcg
     1441 atccggccac cttctgctcg ctcctcgatt cgttcctcaa gcagatgctc ctgaaggaga
     1501 cgaagccggg cgtggccgtc ctcttcccgt gggccgaaac gctgctcccc gagtcgtccg
     1561 gcgaggccgg ggccgaggac cgcgccgtgc gggtcttctt ccagaagtgg gccaccgacc
     1621 cggggttcct ggcggccaac gtgaccttcg tgctcatgac cgagaacctg gccgacatca
     1681 acacgcgcct cgtccgttcg ccgcagacgg tggagcttga ggtcaagcgc cccaacgagc
     1741 aggagcggct cgacttcctg cgctggtcgc gcgatgacgc gtggtttgcc ggcaggtccg
     1801 acctgccccc cgagcggctg gcccagttca ccagcgggct cacccgcatc cagctccgcc
     1861 agatcctggc ctccgtggac gaacaaggcg atcggctgga tgccaggctg ctgcgcgagc
     1921 agaagaaggc ggtcatcgag gccgagtgct tcggcctcct cgaatacgtc gaatcgcccc
     1981 actcgctcga catggtgtcg gggcacgaag gggtcaagca gcgcctgcgg cgcgcggcca
     2041 aggccatcgg cgccgggcgc ctgcgcggcg tgccgatggg ctacctcgtg gcgggcccgg
     2101 tcggatcggc caagacgttc ctcgtcacct gcttcaccgg cgagatcggc tttccgtgcg
     2161 tcaagttcct gaacttccgc tcgcagtggc agggcgtcac cgagggaaat ctcgagaaga
     2221 tcctgaagct gctgcacgcg atgtggccgg tgggcgtcat catcgacgag gccgacgcct
     2281 tcctgggcga tcggaaccag cagggggatt cgggcacgtc caaccgcgtc ttcgcgcagt
     2341 tcgccacgtt catggccaac accgattatc gcggcaagat cgtctggttc ctcatcacct
     2401 cgcgccccga tctcctgccg atcgacctga aacggcaggg gcgcgccgag gagcacctgg
     2461 ccctcttcta tccgcagcac gacgccgaat acgatgcgct cctcaggatc atgctgagga
     2521 agagcggact gaccagcccg gtcacgtcga tccgcgaggt gatcgacgat ccggccgggt
     2581 tgtcgggcgc ggacatcgag gccatcctgg ctcgcgccgc cctggtggcg gatgccgagg
     2641 gggtcggtga cggcaggtcg ctcacggcgg atgtgctgaa gcaggtgttc gccgatttca
     2701 tcccgacatc gaacgcactc gaacgcgaac ttcaggtatt ggtggcggtg caggaatgca
     2761 cctcccgtga aatactgccg gaggtctacc ggagcatgga ccgggccgac cttgcggcac
     2821 gcatcaacga actgcgcgtg ctcctgaacg aggcctgacg gccccgcgcg tcccgctttc
     2881 gaaccggact ctgctccagg aggtgttcgt ggcccgtacg ccgttgttcg actcgctgac
     2941 ccaggcactc cggatcgccg ccttcgcggc ccgggccaaa tcgaacgtgc caccgctgga
     3001 cgaactggtc gacatggcgg gcagccggcg ccgcttcctg aagcaggccg ccgcttcggc
     3061 cgccgccgcg gcgttctacg gttgcgcgca gcccaagcga gacgcgcgcc aggttgcccc
     3121 gcccccccag accgccaccg cggccaaccc ccgcatcgtc atcgtgggtg gcggcatggc
     3181 cggattgaac accgcgtaca agctgcagaa ggccggcctc accgccacga tctacgaagg
     3241 ctccgaccgg accggcggcc gcatgttcac ggccaaagac ctgctgggca ccggcatcac
     3301 caccgagctg gggggggagt tcatcgactc cggccacgcc gagatgctgg ccctgatgca
     3361 ggagttcggc ctcgagtcga tcgacacgca cgcgcccgag gtaaccaatc tcaagaagga
     3421 aacctacttc gtgaacggcc ggcactactc catcgcccag gcggcgcagg agttcgtgcc
     3481 gctggccgcc cggatccagg aacactacga ctcgatgggc gaagtggtcg actaccagac
     3541 cgacggcggc ggcggcacgt tcgaccggat gtcgatcgcg gaatacctcg attcgatcgg
     3601 ctcgaagggg tggctccgcg aggtgctcga cgtggcttac gtcaccgaat atggcctcga
     3661 ggcggccgag cagtcgtcgc tcaacttcct cttcctgatc ggcacgggcg acctgacgga
     3721 tgcctcaacc atcgagctcc tcggcgagag cgacgagcgc ttcaaggtca agggcggcaa
     3781 ccagcgggtg gtggacgaac tggccaggcg cctcgacccg cagatccagc ggcgccacag
     3841 gctggaggcc atcaagccgc gcggcgcggg ctacacgctc accttccagg tcgacggcgc
     3901 cccgcgcgac gtggaggccg acatcgtcgt gctggccatg ccgttcacgc tgctccgcga
     3961 agtcgacatc caggtgccgc tccccgacgt caagacgaag gccatccgcg agctcggcta
     4021 cggtacgaac gccaaggtgc tggtcggctt caaggatcgc ccctggatca ggaaggggta
     4081 ctccggcgcc acctacagcg acgagatgtt ccagctctgc tgggacaaca gcttcctgca
     4141 ggccggaacg gaaggaggaa tcaccctcta ttccggcggc aagctcggcc tcgacgccgg
     4201 gaacggcacc gccgaggaag cggccgcgcg actcatgccg ggcttcgagc gtacgtaccc
     4261 gggcgcgtcg gcgcgacgga acggcaggac ctcccgtttc cactggccca ccttcccgtg
     4321 gacgaaggcc agctatgcct gctacaagcc gggacagtgg accaccatcg ccggggcgga
     4381 aggagtgccg gtgggcaatc tcttcttcgc cggggagcac tgcagttacg acttccaggg
     4441 ttacatgaac ggcgccgcgc agtcggggct cgacacggcc aaggcggtga tgggtctgct
     4501 cagcgggtcg cccgtcgatt cgggaatgct gttcgagcgc gaagccgcgc tcgtcggttg
     4561 agggaggaac tcgatgaagg ttcggggatc ggaatccggg gctgtccacc tgacggtggc
     4621 cggccggctg ctcatcatgg tcgtggggct ggccgtgctg ggttacgtgg cgtacatgta
     4681 ccgggatcgg cttcccttca agccgggagc ccccaagtcg aatacgagca cgacctcgcg
     4741 caccgatgac gcgcacccct ccggcggcgc cgcgccggcc gcctcgcagg gaggcgtgat
     4801 ggcccgcatc cgcaacgccg gagtcgtccg cgtggggatg gagcccgacg ccgcgccgct
     4861 tcacttcgtc aacgatcgca agcaggaaga cggtttcgat ttccgcctgg ccggcgtcat
     4921 cgccgacgca ttgaagtcgg gcacgcgtgt ccaggtggtg gaagccgact acgaagacct
     4981 ccccggccaa ctcacggcgg gcaatatcga cctcatcatg gccggttacg tgcccgaccc
     5041 ctccatcgac ggcgtggact ggtccaacgg ctatctcgac ttcggcctct gcatgatcgt
     5101 ggcggaagga aacgtctccc agttccgctc ccccgccgac ctggccggca aacgcgtggc
     5161 catctacgac gatcccgccg ccgagcgctg ggtggaaagc aacatcccca acgcgcgcat
     5221 cagcaaattc tcgggcgaca gcggctggtt cgaggccgtg gaagacgaca aggccgacgc
     5281 gttgatctac gactacccct tcgccgccga ggagatcaag gagcacccgc gcacggtgat
     5341 cgtgcgttac aacctgaacg catcgaagta tgccgtcggc atccccaagg ggaattacga
     5401 cctggtgtac gaggtgaacc aggccatcga gaactt
//
LOCUS       DASVOD010000417         5208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_967818, whole genome shotgun sequence.
ACCESSION   DASVOD010000417 DASVOD010000000
VERSION     DASVOD010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967818"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /gene="alc"
                     /locus_tag="VF720_09555"
     CDS             complement(<1..1463)
                     /gene="alc"
                     /locus_tag="VF720_09555"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HEX7879643.1"
                     /translation="MSTKALPGIELSAERLGGRVLAANDDFFAPKENLLKAAAPIWLE
                     DEYTDRGKWMDGWETRRRREPGNDWCVMRLGLAGVIRAVVVDTAFFRGNFPESFSLDG
                     ASVEDDDAAVAPGAVWTPILPATPLSGNTVHRFPVDSPWRFTHLRLNIFPDGGVARFR
                     AYGAAIPDRGLVEQFDGRIDLAGMVNGGEAIAASDMFFSDRNNMLRTGASTHMADGWE
                     TKRRRGPGHDWAVVRLGARGTIESAIIDTTHFKGNAPGRAKLEAIDAPGMADDAVLRR
                     DDWRTLLPETPLQPHDAREFNADALVESGSATHVRISIYPDGGVARLRLYGKPTPDGV
                     EALGLTWLNTLPPAELEERLGRCCAAPAWIRALAAGRPYTSRAALDAASEKALAGLDR
                     AEVLEALTRHPRLGERQAAVSGGEVESRWSRREQSGVSAASDDVKVTIGELNAAYEAK
                     FGWIFLLCAAGLSGEQVVAAMKERMTNDKDAEASVAG"
     gene            complement(1474..1809)
                     /locus_tag="VF720_09560"
     CDS             complement(1474..1809)
                     /locus_tag="VF720_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7879644.1"
                     /translation="MSLAAVWTGASVRGFGLERLAEWMAAAPARLAGLDDRKGAIVPG
                     RHADLVVFDPDEPFRVQGSALSHRHPLTPWEGRLLKGVVQNTWLRGRLVYDRNADPSG
                     PSTPAGRLL"
     gene            complement(1806..2810)
                     /gene="allB"
                     /locus_tag="VF720_09565"
     CDS             complement(1806..2810)
                     /gene="allB"
                     /locus_tag="VF720_09565"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HEX7879645.1"
                     /translation="MSPSFSLASRRLVLPDGVRPATVVVRDGVIVEVTPGIQPGARDL
                     GELVVLPGLVDTHVHINEPGRTDWEGFATLTTAAAAGGITTLVDMPLNSIPATTTPDA
                     LELKRAAAEGKVRVDVGFWGGLVPDNIDELPGLARAGVRGFKAFQVHSGVDEFPDVPV
                     ETMDEALEILEPLGLPLLVHAEDPAVVNWQVNLLLENPVSHAAWAASRAFAEEAAIDR
                     LVTIAARHATRLHVVHVSTLGGVERIRKARARGVPITGETCPHYLTWCGDEVPDGATR
                     YKCAPPLGSFEDRDALWRGLDDGTLGMVVTDHSPCPPELKHEDQGGSTGRGAASPRCR
                     "
     gene            2904..4424
                     /locus_tag="VF720_09570"
     CDS             2904..4424
                     /locus_tag="VF720_09570"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7879646.1"
                     /translation="MPWLILVIALLGTPFEVTRAAEPASTTTPSEHTRQEATVRQLVG
                     EALLSRWGYDHLRELCDTVGHRLAGSPAMARAVTWSEGAMREAGLVNVHREPARVPDW
                     TRGPESARIVEPIEKPLTMLGLGRSVSTPTGGITAPVVVVRSYDELNALGTAGVAGRI
                     VLYDFAWTTYSAASEFRTKGTSKAAALGAVGMLVRSATNRSHDTPHTGRLVYDPAQPK
                     IPAASLSVESAGLIRRLTESGADVSVHLEMDHQVGTTRDCWNVIGEVPGSAKPEEIVL
                     INGHLDSWDVGQGAQDDGIGCLMMLEAARLIANLPERPRRTVRVAFFTDEEISGGGTG
                     SYLEHHAAELPLHVAGFESDAGGEVATGFSIDVRGVAVMRDSSGTMVATADTLRSFRE
                     AATLRGDILKSLAGWSWALGPLGARKLVAGGVGADLDELTKFGLLGFGVSHPTEADYF
                     DYHHSPADTFDRVDPDKMARNAAILAVAIYLAADAPERLLPWPAQPNAPAYPSGTR"
     gene            4544..>5208
                     /locus_tag="VF720_09575"
     CDS             4544..>5208
                     /locus_tag="VF720_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008485426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7879647.1"
                     /translation="MFREREAGTLSGWMMLLLLLAALFATGAWLVSSIRAHEAGQLIL
                     AVVSLTVVGVSLGGLTIVNPNEAVVATLFGSYLGSIKRPGFWWINPFSSKRRLSLKVR
                     NFESSKLKVNDHDGNPIEIAAVVVWKVVETAEAVFHVDDYNNFVHVQSESALRNLATS
                     HPYDAHNDGQMSLRSHTVEIAANLKSEIQERLLQAGVEVIEARISHLAYAPEIASAML
                     QRQ"
BASE COUNT          820 a         1851 c         1738 g          799 t
ORIGIN      
        1 atgccggcca cgctcgcctc ggcgtccttg tcgttggtca tccgctcctt cattgccgca
       61 acgacctgct cgcccgagag gccggcggca cacaacagga agatccatcc gaatttcgcc
      121 tcgtacgcgg cattgagttc gccgatcgtc accttcacgt cgtccgaggc cgcggacacg
      181 cccgactgct cgcgccgtga ccagcgcgat tccacttctc ctccagaaac cgccgcctgc
      241 ctctctccga ggcgcgggtg gcgggtcagc gcttcgagaa cctccgcccg gtcgaggccg
      301 gcgagggcct tttcactcgc ggcgtcgagg gccgcgcgtg acgtgtaggg ccgcccggct
      361 gccaacgccc tgatccatgc cggcgcggca cagcaacggc cgaggcgttc ctcgagctcg
      421 gccggcggca gtgtattcag ccaggtgagg ccgagtgcct ccacgccgtc cggcgtgggc
      481 ttgccgtaca ggcggagccg ggccacgccc ccatccgggt agatcgagat ccggacatgc
      541 gtggcggatc cactctccac cagtgcgtcc gcgttgaact cccgcgcgtc gtggggctgc
      601 agcggcgtct cgggcaggag cgtacgccag tcatcacggc ggagcaccgc gtcgtcggcc
      661 atcccgggcg cgtcgatcgc ttccagcttc gcccgtcccg gcgcgttccc cttgaagtgc
      721 gtcgtgtcga tgatcgccga ctcgatcgtt ccgcgcgcgc cgagccggac cacggcccag
      781 tcgtgaccgg gtccccgacg acgcttcgtt tcccatccgt cagccatgtg ggtggaggct
      841 ccggttcgga gcatgttgtt ccggtcggaa aagaacatgt cgctggccgc gatcgcctcg
      901 cccccgttca ccatccccgc caggtcgatg cggccgtcga actgctccac gagcccccgg
      961 tccgggatgg cagcaccgta ggcccgaaag cgcgccacgc cgccgtcggg aaagatgttg
     1021 aggcgcaggt gggtgaagcg ccagggcgag tcgaccggga aacggtgaac cgtgttcccc
     1081 gaaagcggcg tggccggtag gatcggcgtc cagacggcac cgggcgccac cgcggcatcg
     1141 tcatcctcga cggaggcgcc gtcgagtgag aacgactcgg gaaagttgcc ccggaagaag
     1201 gccgtgtcca ccaccaccgc ccggatgacg ccggccaggc cgagccgcat cacgcaccag
     1261 tcgttgcccg gctcccgccg gcggcgggtt tcccacccat ccatccactt gccgcggtcg
     1321 gtgtactcgt cctccagcca gatgggtgcg gcggccttga ggaggttttc cttgggtgcg
     1381 aagaagtcgt cgttggcggc cagcacgcgg ccgccgaggc gttcggcgga aagctcgatg
     1441 ccgggcaggg ctttggtact catcgcggga aaactacagg aggcgcccgg ccggcgtcga
     1501 tggcccggaa ggatcggcgt tgcggtcgta cacgagccgg ccgcgaagcc acgtgttctg
     1561 cacgaccccc ttcagcaggc gcccttccca cggcgtgagc gggtggcggt gcgagagggc
     1621 gcttccctga acccggaacg gctcgtccgg atcgaacacg acgagatccg cgtggcgccc
     1681 cgggacgatg gcccccttcc ggtcgtcgag ccccgccagg cgggcgggtg ccgccgccat
     1741 ccattccgcc agtcgctcga gcccgaagcc ccggaccgaa gccccggtcc acaccgcggc
     1801 caggctcacc tgcagcgagg cgatgccgcc ccacgcccgg tcgaacctcc ctggtcctcg
     1861 tgcttgagtt ccggcggaca gggggaatga tcggtcacga ccatcccgag cgtgccgtcg
     1921 tcgagaccgc gccacagggc gtcgcggtcc tcgaagctcc cgagaggcgg cgcgcacttg
     1981 taacgggtgg cgccatccgg cacctcgtcg ccgcaccagg tgaggtagtg gggacacgtc
     2041 tccccggtga tgggcacgcc gcgggcccgc gccttgcgga tccgctccac gccgccgaga
     2101 gtggagacgt gcaccacgtg gagccgcgtg gcgtgcctgg ccgcgatggt cacgagccgg
     2161 tcgatggccg cctcctcggc gaacgcccgg gaggccgccc acgccgcatg tgagactggg
     2221 ttctcgagca ggaggttcac ctgccagttc acgaccgcgg gatcctcggc atgaacgagc
     2281 aacgggagcc cgagcggctc gaggatttcg agcgcctcgt ccatcgtctc caccggcaca
     2341 tcggggaact catccacgcc cgagtgcacc tggaaggcct tgaagccccg gacaccggcg
     2401 cgggccaacc ccggcagctc gtcgatgttg tccggaacga gcccgcccca gaaccccaca
     2461 tccacccgca ccttcccctc cgccgccgcg cgcttcaact cgagggcatc cggcgtggtt
     2521 gttgccggaa tggagttgag cggcatgtcc accagcgtgg tgatgccgcc ggcggccgcc
     2581 gccgtcgtga gcgtggcaaa cccttcccag tcggtgcggc ccggttcgtt gatgtgcacg
     2641 tgggtgtcga ccagcccggg gagtactacc agttccccca aatcgcgcgc gccgggctgg
     2701 atgcccgggg tgacttcgac gatgacgccg tcacgcacga cgacggtggc ggggcgcaca
     2761 ccatcgggga ggacaaggcg gcgcgaagcg agcgagaagg aggggctcat ctggttcccg
     2821 gcgtggattg acgatggcga tcgggtcacg cagagtaggc cggaaagttg ccgtcgtcgt
     2881 caacatccca cacggggagt cgcatgccct ggctcatcct cgtcattgcc ctcctcggca
     2941 cgccgttcga ggtcacgcgg gccgccgagc ccgcctcgac gacgacgccg tccgagcaca
     3001 cccgccagga agccaccgtc cgccagctcg tcggcgaggc gctcctctcc cgctggggtt
     3061 acgaccatct gcgtgagctg tgcgacacgg tcggccaccg cctggccggc tcccccgcca
     3121 tggccagggc cgtcacctgg tccgaagggg ccatgcgcga agcggggctc gtcaacgtgc
     3181 accgcgagcc ggcgcgcgtg cccgactgga cgcgcggccc cgagagcgcc cggatcgtgg
     3241 agccgatcga gaagcctctc acgatgctcg ggctggggcg ttcggtgtca actccaaccg
     3301 gcgggatcac ggcgccggtg gtcgtggtcc gttcctacga cgagttgaat gccctcggca
     3361 cggcgggcgt ggccggccgg atcgtcctgt acgacttcgc ctggacgacc tactccgccg
     3421 catcggaatt ccggacgaag ggcacctcga aagcggccgc gctgggcgcg gtggggatgc
     3481 tcgttcgttc ggccaccaac cggagccacg acacgccgca caccggccgc ctcgtgtacg
     3541 acccggccca gccgaagatc ccggccgcgt cgctgagcgt ggaatcggcc gggctcatcc
     3601 gcaggctcac cgaatcaggc gccgacgtca gcgtgcatct cgagatggac catcaggtcg
     3661 ggacgacgcg cgattgctgg aacgtgatcg gcgaggttcc cggcagcgca aagcccgaag
     3721 agatcgtcct gatcaacggc cacctcgatt cctgggacgt ggggcaggga gcccaggacg
     3781 acggcatcgg ctgcctcatg atgctcgagg cggcccgtct catcgcgaac cttcccgagc
     3841 gcccccgccg gaccgtgcgc gtggcgttct tcaccgacga ggagatatcg ggcggcggaa
     3901 ccggttccta tctcgagcac cacgcggcgg agcttcccct ccacgtggcc ggcttcgagt
     3961 ccgacgcggg cggggaagtg gccaccggtt tctcgatcga cgtgcgcggc gtggccgtca
     4021 tgcgcgacag ctcaggcacg atggtggcca cggccgacac gctccgttcc ttccgcgaag
     4081 cagcgaccct ccggggcgac atcctgaaat cactggccgg ctggagctgg gcgctcggcc
     4141 cgttgggcgc caggaagctg gtggccgggg gcgtgggcgc cgacctcgac gagctcacga
     4201 agtttgggct gctcggcttc ggggtgagcc atcccaccga agcggactac ttcgattacc
     4261 accactcgcc ggccgacacc ttcgaccggg tcgacccgga caagatggcc cgcaacgccg
     4321 ccatcctggc cgtggccatt tacctggccg ccgatgcacc ggagcgcctg ctcccctggc
     4381 cggcccagcc gaacgccccc gcctacccgt ccggcacccg ctgacccgcc gcccgcccgg
     4441 ggcgcgccac ctgcaacctg ccggaaattc ccgcgtaggt actggcatca aaacagatcg
     4501 ttttgatatc attaagatgt caccaacaaa caaggaggat tggatgttcc gcgaacgtga
     4561 ggccggcacg ctgtcgggat ggatgatgtt gctcttgctg ctcgccgctc ttttcgccac
     4621 gggggcgtgg ctcgtttcct ccatccgcgc ccacgaggcc ggacagctga tcctggcggt
     4681 cgtgagcctc accgtcgtcg gcgtcagcct cggcgggctc accatcgtca accccaacga
     4741 ggcggtcgtg gccactctct tcggcagcta cctggggtcg atcaagcgcc ccggcttctg
     4801 gtggatcaat cccttcagct cgaagcggcg cctttcgctc aaggtgcgca atttcgagag
     4861 cagcaagctg aaggtcaacg atcatgacgg caacccgatc gagattgccg ccgtggtcgt
     4921 ctggaaggtg gtcgaaacgg ccgaggcggt tttccacgtg gatgactaca acaacttcgt
     4981 ccacgtccag tcggagtcgg cgctgcggaa cctggccacc agtcacccct acgatgcgca
     5041 caacgacggt cagatgtcgc tgcgcagcca caccgtcgag atcgccgcga acctgaaatc
     5101 ggaaatccag gagcggctgt tgcaggccgg cgtggaggtc atcgaggccc gcatcagcca
     5161 cctggcttac gcgcccgaga tcgccagcgc catgctgcag cggcagca
//
LOCUS       DASVOD010000418         7928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_914853, whole genome shotgun sequence.
ACCESSION   DASVOD010000418 DASVOD010000000
VERSION     DASVOD010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914853"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..950)
                     /locus_tag="VF720_09580"
     CDS             complement(<1..950)
                     /locus_tag="VF720_09580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7879648.1"
                     /translation="MTLTLAAGTRLGPYEILSPLGAGGMGEVYRARDTRLGRDVAVKV
                     LPEHLSANPDVRARFEREAKTVSSLNHPNICTLFDVGREGDTDYLVMELVEGDTLDAR
                     LRRGPLPPADLLRIGRQVADALDRAHRAQVIHRDLKPGNIMLTKSGAKLMDFGLARAA
                     GAAAGSGSGVAMSALTHTPTVAQALTTQGTLVGTFQYMAPEQLEGGEADQRSDIWALG
                     CVLYEMASGRRPFDGRSQASLIAAILEREPAPLAEAPSGSTSVAGAIPTGLGRIIRNC
                     LAKDPDDRIQTAHDVKLQLDGVAEGMGLSNLVTPVPGAGP"
     gene            complement(999..2816)
                     /locus_tag="VF720_09585"
     CDS             complement(999..2816)
                     /locus_tag="VF720_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7879649.1"
                     /translation="MSRDRFALGRRTIVSARFLPLLILTALVLPAVTASQARAVPLTV
                     TAVNPPINGVGAIYAPVRITFDRAVMTSTINGNNIRVFGKGSGAPAGGFSITEGGKVV
                     TFTPTRAWGAGETVTVNIGTGVRSSTGDNLVAGGFAWNFRVTCAPASINYLLIDTISN
                     RTTPVTQTRIYGAASCDFNKDGSVDLATVNEVSADIRVFLNRDDGSGLMTTPFLPPQP
                     IGDEASPNDVGDFDNDGNPDIVAAATGTDDVWVMLGNGDGTFDITGLDVGQNPHGIVT
                     LDLDGDADLDIAVACASSGNIWILVNNGVGNFSAVAPVDAGVTTEYGLAAGDMNRDGR
                     IDLVVGGRDGPHIRTLLNNGNVTFTPAGPPQDSGGSTWVVTLGDLNGDGNLDATVANS
                     FSGNGARLLNLGAGTFGPPAFSITSSHTVSTDLGDLDGDGDLDWILASYGGGFWRILR
                     NDGAGNFTTFANITATSNPSCAIILDLDNDLDLDLALTDEIADVIQLRKNTRSSSVPT
                     PEPGGGRVQLAPNAPNPFATSTTIRYHLAAAGSARLTLFDARGAARFERDLGAGQEGW
                     NEVSFEAVDPTGQPLPSGIYFYEVVIGNDRARGKLTIRR"
     gene            complement(2890..3384)
                     /locus_tag="VF720_09590"
     CDS             complement(2890..3384)
                     /locus_tag="VF720_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879650.1"
                     /translation="MTVWRLNAHPSDAGRTARWFTRDCLVAMGWGDVGDLRRASPSSS
                     TSIAGMIRRADPDSTDSQLGGQALWCFFHDMVVGDLVLVNDGSNRDVVMRIEGPYEWS
                     GQVSPVAPGDYQHQRRATLTAIDAGELWASCGSRIAEGEDRRWRLARCAAPAVPVGEK
                     AKVD"
     gene            complement(3490..3726)
                     /locus_tag="VF720_09595"
     CDS             complement(3490..3726)
                     /locus_tag="VF720_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879651.1"
                     /translation="MSATPECLRCRGPMEPGFVLDRSHGGSYTTQSWIEGEAEKSFWF
                     GLKTGGREKFGVRAFRCEKCGYLEFYANAKAEKS"
     gene            complement(3862..4614)
                     /locus_tag="VF720_09600"
     CDS             complement(3862..4614)
                     /locus_tag="VF720_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX7879652.1"
                     /translation="MRWALFSDIHGNLEALEVVLDSIRKARVDRMVCLGDIVGYGASP
                     NECVGLVKGSGATTIAGNHDHAALGLLDIGEFNDHARAAILWTQEAITPETAAWLRER
                     EMVVAEEGIRIVHASPGHPAEWNYILTPMDAAAEFRTFEEPVCFVGHSHVPGLFLGPS
                     AEDAIFAYVQESMFPANEPITLPVGRRAILNVGSVGQPRDRDPRAAWLLYDDETGTVI
                     LNRLMYDLEAAQHRILEAGLPPFLAYRLGMGS"
     gene            complement(4638..5051)
                     /gene="nusB"
                     /locus_tag="VF720_09605"
     CDS             complement(4638..5051)
                     /gene="nusB"
                     /locus_tag="VF720_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX7879653.1"
                     /translation="MNGPRRRSRIAAMRALFQMQVVEDPIEPVVAAMRSDAELSEDIA
                     SYGAHLVTLFHLNHDEVDKAIAEALDRWELKRVAQVDRAVLRIAATELLYVQDVPAKV
                     AIDEAIEIAGRYGSNQSGAFVNGVLDRLARAHREL"
     gene            complement(5048..5665)
                     /gene="ribH"
                     /locus_tag="VF720_09610"
     CDS             complement(5048..5665)
                     /gene="ribH"
                     /locus_tag="VF720_09610"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX7879654.1"
                     /translation="MPQELSGALDASGRRYGIVVARFNEFFTRRLMDGAVDALLRHGA
                     DEESLAIVWVPGAWEVAPAVRAMALSGRFDAVIGLALVMKGETSHDTIVAGEAASGIA
                     EAQRVSGIPCPLGIVSAENLDQATERCGSKQGNRGWDAAMAAIEMVNLLPALASTDES
                     ARSGESPNGSSTSSTPRVNGSRNYPVSNAARAKAARFSRKKPASR"
     gene            complement(5668..6888)
                     /locus_tag="VF720_09615"
     CDS             complement(5668..6888)
                     /locus_tag="VF720_09615"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX7879655.1"
                     /translation="MSTETTTFATIDEAIADLVAGKMVIVMDDDDRENEGDVIMAAEK
                     ITPEAVNFIARFARGLICVPMTEAGLERLGLDMMVETNTAPHGTAFTVSVDYLIGTTT
                     GISAHDRAATIKALADPGSRPQDFGRPGHIFPLKAAEGGVLRRAGHTEAVIDLLTLAD
                     LQPVGLLCEILDDDGSMARAPRLHRFAAEHQLKIITIKDLIAWRYRRNSLVKEIVTTT
                     LPTKFGSFELHLYASTADGQEHMALVKGKVAGDEPVLVRMHSQCLTGDLFHSLRCDCG
                     DQMEKALERIEAEGKGVFVYMRQEGRGIGLSNKLKAYNLQDQGLDTVEANEKLGFAPD
                     ARDYGVGAQILAELGLKRIRLLTNNPKKRVGLESYGIQIVEQVPIEIAPNAVNRRYLE
                     TKRTKLGHLLNGGQ"
     gene            complement(6885..7502)
                     /locus_tag="VF720_09620"
     CDS             complement(6885..7502)
                     /locus_tag="VF720_09620"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX7879656.1"
                     /translation="MFTGLITEVGRIERATEIPNGVELIIRAPRTAARLSIGASLAVN
                     GVCLTITKLDPPHATVQAVGATMTTTTFGRDLLREGARVNLEPSLRAGDELGGHLVAG
                     HVDGVGTVVAIKPDGEAVWLTINVPNELSRYVARRGSIAIDGVSLTVAALEDAPDGAR
                     ITMSIIPHTLKETICGEYQPGRPVNIEVDTVARYLERLLPSEGRS"
     gene            complement(7503..>7928)
                     /locus_tag="VF720_09625"
     CDS             complement(7503..>7928)
                     /locus_tag="VF720_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7879657.1"
                     /translation="RSSPRAAWLADDGIRRIVVCGPAAAASRQRAFAATGAEVWPMAV
                     RPDRHGRSGIQLSMLLKRAAREGITSVLVEGGGRLAGQFLTGKQADRLALVTAPVLLG
                     GDSRRWTNGLGVTRVDRAPRLRNVALRPIGTDWLVTGDL"
BASE COUNT         1172 a         2617 c         2804 g         1335 t
ORIGIN      
        1 atcggcccgg cgccgggtac cggcgtgacc aggttcgaca accccattcc ctcggccacg
       61 ccgtcgagct gcagcttcac atcgtgggcc gtctgaatgc ggtcatcggg atccttggcc
      121 aggcagttcc gaatgatgcg cccgaggccg gtcggaatgg cgccggccac gctggtgctg
      181 ccggaggggg cctcggccag cggcgccggc tcgcgctcga ggatggcggc gatgagcgac
      241 gcctggctcc gaccgtcgaa cgggcgacgg ccgctggcca tttcgtacag cacgcaaccg
      301 agcgcccaga tatcgctgcg ctggtcggcc tcgcctccct cgagctgctc gggggccatg
      361 tactggaagg tgccgaccag cgtcccctgt gtggtcagcg cctgggccac cgtcggcgtg
      421 tgggtgagtg cgctcatggc cacgcccgaa ccggagccgg cggccgcgcc cgccgcccgg
      481 gcgaggccga agtccatcag cttggcgccc gacttcgtga gcatgatgtt gccgggcttc
      541 aggtcgcgat ggatgacctg cgcgcgatgg gcccggtcaa gcgcgtcggc cacctgtcgc
      601 ccgatgcgga ggaggtcggc gggcggaagc ggcccgcgcc ggagccgcgc gtcgagcgta
      661 tccccctcga ccagttccat caccaggtag tcggtgtccc cctcgcgccc cacgtcgaac
      721 agcgtgcaga tgttcggatg gttgaggctg gagacggtct tcgcctcgcg ctcgaaacgc
      781 gcgcggacgt ccgggttggc ggagagatgt tccggcagca ccttgaccgc cacatcgcgg
      841 ccgagccgcg tgtcgcgggc ccggtaaacc tcccccatgc cgccggcgcc gagcggggag
      901 aggatttcgt aaggtccgag acgggtaccc gcggccaggg tcagggtcat gggggcgcat
      961 ggtagcggct catccggcac cccaccagag gaggcggatc agcgccggat cgtgagcttt
     1021 ccccgggccc gatcgttgcc gatcaccacc tcgtagaagt agatgcccga cgggagcggc
     1081 tggcccgtcg ggtcgaccgc ctcgaacgac acctcgttcc acccctcctg gcccgccccg
     1141 agatcccgct cgaaccgtgc ggcgccccgg gcgtcgaaca acgtgaggcg cgcggatccg
     1201 gccgccgcca ggtggtagcg aatggtcgtg ctcgtggcga aggggttcgg cgcgttgggc
     1261 gcgagctgaa cgcggccgcc acccggctcc ggtgtcggca cggacgagct gcgcgtgttc
     1321 ttccgcagct ggatgacgtc ggcgatctcg tccgtgagcg ccaggtcaag gtcgaggtcg
     1381 ttgtccaggt cgaggatgat ggcgcacgac gggttgctgg tggcggtgat gttggcgaag
     1441 gtggtgaagt ttcccgcgcc gtcgttcctc aggatccgcc agaagccgcc gccgtagctg
     1501 gccaggatcc agtcgaggtc gccgtcgccg tccaggtcgc ccaggtcggt ggagacggtg
     1561 tgggagctgg tgatggagaa ggccggtggt ccgaacgtac cggcgccgag gttcagaagg
     1621 cgcgcgccgt ttcccgagaa gctgttggcc accgtggcgt ccaggttgcc gtcgccgttc
     1681 aggtcgccca gcgtgacgac ccaggtggag ccgccggaat cctggggcgg cccggccggc
     1741 gtgaaggtaa cgttgccgtt gttgaggagc gtgcggatgt gggggccgtc gcgcccgccg
     1801 accacgaggt cgatccgtcc gtcccggttc atgtcgccgg cggccaggcc gtactcggtg
     1861 gtcacgccgg catcgacggg cgccacggcg ctgaagttcc ccacgccgtt gttgaccagg
     1921 atccagatgt tgccgctcga ggcgcaggcc acggcgatgt cgaggtcggc atcgccgtcc
     1981 aggtcgagcg tcacgatccc gtgcgggttc tgtcccacgt cgaggccggt gatgtcgaac
     2041 gtgccgtccc cgttgccgag catcacccag acgtcgtcgg tgccggtggc ggcggccacg
     2101 atgtcggggt tgccgtcgtt gtcgaaatcg cccacgtcgt tcgggctggc ctcgtcgcca
     2161 atgggctggg gcggaaggaa cggcgtggtc atgagcccgc tgccgtcgtc gcggttcagg
     2221 aatacgcgga tgtccgccga cacttcgttc accgtggcca ggtcgaccga gccgtccttg
     2281 ttgaagtcgc acgaggcggc gccgtagatg cgcgtctggg tgaccggcgt cgtgcgattg
     2341 gaaatggtgt cgatgagcag gtaattgatg ctggcgggcg cgcaggtcac ccggaagttc
     2401 caggcaaagc ctccggccac gagattgtcg ccggtggacg accggacccc ggtgccgatg
     2461 ttgacggtga cagtctcgcc ggcgccccac gcgcgggtgg gggtgaaggt gacgaccttc
     2521 cccccctcgg tgatggaaaa cccgccggcc ggcgcgccgc tccccttgcc gaacacccgg
     2581 atattgttgc cgttgatcgt ggaggtcatg acggcgcgat cgaacgtgat ccggaccggc
     2641 gcgtagatgg cccccacccc gttgatcggc gggttgacgg cggtgacggt gagggggacg
     2701 gcccgggcct gggatgcggt gacggcgggg agaaccaggg cggtgagaat caggaggggg
     2761 agaaacctgg cggagacgat cgtgcggcga ccgagtgcga acctatcccg cgacatggcg
     2821 caagacctcc ggagcgatgc gatcagtagg aaacggtcac ggcgtccggg gaggatatca
     2881 ccgccggtgt cagtcgacct tggccttctc ccccaccggc acggccgggg ccgcacagcg
     2941 ggccaggcgc cagcggcggt cttcgccctc ggcgatccgg ctgccgcacg aggcccagag
     3001 ctcgcccgcg tcgatggcgg tgagcgtggc gcggcgctgg tgctggtagt cgcccggagc
     3061 caccggtgac acctggccgg accactcgta ggggccttca atccgcatga cgacatcgcg
     3121 gttcgatccg tcgttcacga ggaccaggtc gcccaccacc atgtcgtgga agaagcacca
     3181 gagagcctgc ccaccgagct ggctgtcggt ggagtcgggg tcggcccggc ggatcatgcc
     3241 ggcgatcgag gtggaggagc tgggcgatgc ccggcgcagg tcgcccacgt cgccccaccc
     3301 catggccacg aggcaatcgc gggtgaacca tcgcgcggtt cgacccgcgt cacttggatg
     3361 cgcgttcagg cgccagaccg tcatgtcgtc ccctgctcct ggggcgggct cggccggccg
     3421 gtcatcgcgt tccccggtgc ggcgctcagg gcccccaatc tccccaagac agggggatca
     3481 tcgcacagat caggacttct cggccttggc gttcgcgtag aactcgaggt aaccgcactt
     3541 ttcgcaccgg aacgcgcgga cgccgaactt ctcgcgcccc ccggtcttca ggccgaacca
     3601 gaaactcttt tcggcttcgc cttcgatcca gctctgcgtg gtgtacgagc cgccgtgcga
     3661 gcgatcgagg acgaagccgg gctccatcgg gccgcggcag cggaggcatt cgggggtggc
     3721 gctcatggtg gtttcccggg ttgggccggt cgggccggcc gtgggtgagg cgcgactgtg
     3781 gcatgccaca gttgggtgat atcgaatcag tgtacgcgcg gggctctcgg gagtcgcgcg
     3841 gggcgatcgc gggttgcggg cctacgaccc catccccagc cgatacgcca ggaacggcgg
     3901 caatcccgct tcgaggatcc ggtgctgggc cgcctcgagg tcgtacatga ggcgattcag
     3961 gatgacggtg ccggtctcgt cgtcgtacag gagccaggcc gcgcgcgggt cgcgatcgcg
     4021 gggctggccg accgagccca cgttgaggat ggcgcgccgg ccaaccggaa gggtgatcgg
     4081 ttcgttggcg gggaacatcg attcctggac gtaggcgaag atggcgtcct cggcgctcgg
     4141 tccgagaaag aggccgggca catgcgagtg cccgacgaag cagaccggct cctcgaacgt
     4201 gcggaactcc gcggcggcgt ccatcggcgt caggatgtag ttccactcgg ccgggtggcc
     4261 ggggctggcg tgcacgatcc ggatcccctc ttccgccacg accatctcgc gctcgcgcag
     4321 ccacgcggcc gtctcgggcg tgatggcttc ctgagtccag aggatggcgg cccgggcatg
     4381 gtcgttgaac tcgccgatgt cgaggagtcc gagcgcggcg tggtcgtgat tgccggcgat
     4441 tgtggtcgcg cccgacccct tcaccagccc cacgcactcg ttcgggctgg ccccgtagcc
     4501 gacgatatcg ccgaggcaga ccatgcgatc gaccctggcc ttgcggatgg aatcgagcac
     4561 gacctcgagt gcttcaaggt tgccatgaat gtccgaaaag agtgcccaac gcatgtcagc
     4621 ggccccgtgg tgtcatgtca aagttcgcga tgcgcgcggg cgagtcggtc gagcacgccg
     4681 ttgacgaagg cgccgctctg gttcgatcca tagcgcccgg cgatctcgat ggcttcgtcg
     4741 atcgccacct tggccggaac gtcctggacg tagagaagct ccgtggccgc gatccggaga
     4801 accgcccgat cgacctgcgc cacgcgtttc aactcccacc ggtcgagcgc ctcggcgatc
     4861 gccttgtcca cctcgtcatg attgagatga aacagcgtga ccaggtgcgc cccgtagctc
     4921 gcgatgtctt ccgagagctc ggcatcgctt cgcatggcag ccacgaccgg ctcgatcggg
     4981 tcctccacga cctgcatctg gaagagggcc ctcatggcgg cgatacggga gcggcggcgg
     5041 ggtccgttca tcgggacgcg ggcttctttc ggctgaagcg cgcggctttc gcgcgcgcgg
     5101 cgttgctcac ggggtaattg cgcgatccgt tgacacgcgg agtggacgat gtcgaagaac
     5161 cattcggtga ctcgccgctt cgcgccgact catcagtact ggccaacgcc ggcagcagat
     5221 tgaccatctc gatggccgcc atcgccgcgt cccagccacg gtttccctgc ttgctgccgc
     5281 agcgctcggt ggcctggtcg aggttttctg cgctcacgat gccgagcggg cacgggatgc
     5341 cgctcacgcg ctgggcttcg gcgatgccgg aggcggcttc gccggccacg atcgtgtcgt
     5401 ggctcgtctc ccccttcatg acgagcgcga ggccgatcac cgcgtcgaag cggccggaga
     5461 gggccatggc gcggacggcc ggggccactt cccaggcgcc gggcacccag acgatggcca
     5521 ggctctcttc gtcggcgccg tggcggagga gcgcgtccac cgcgccgtcc atgagacggc
     5581 gcgtgaagaa ttcgttgaag cgggccacca cgattccgta gcgccggccg gacgcgtcga
     5641 gcgcccccga gagttcctgg ggcatgacta ctgacctccg ttcaagagat gaccgagctt
     5701 ggtccgcttt gtttcgaggt atcgccggtt gacggcgttg ggggcgattt cgatgggcac
     5761 ctgttccacg atctggatgc cgtaggactc gagtccgacc cgttttttcg gattattcgt
     5821 cagaaggcgg atccgtttca atccgagctc ggccaggatc tgggcgccga cgccgtaatc
     5881 gcgcgcgtcg ggcgcgaatc cgagcttctc gttggcctcg acggtgtcga gcccctgatc
     5941 ttgtagatta taggctttca gcttgttgga gaggccgatg ccacgcccct cctggcgcat
     6001 gtagacgaac acgcccttac cctcggcctc gatccgttcg agcgccttct ccatctggtc
     6061 gccgcagtcg cagcgcaggc tgtggaacag atcgcccgtg aggcactggc tgtgcatgcg
     6121 gaccagcacg ggctcgtccc ccgccacctt tcccttcacc agcgccatgt gctcctgacc
     6181 gtcggcggtg gaggcgtaca ggtggagctc gaacgagccg aacttcgtcg gcagcgtggt
     6241 cgtgacgatc tccttgacca gcgagttgcg ccggtagcgc caggcgatga ggtccttgat
     6301 cgtgatgatc ttgagctggt gctccgccgc gaagcggtgg agccgcgggg cgcgcgccat
     6361 cgagccgtcg tcgtcgagaa tctcgcagag gagtccgacc ggctgaagat cggccagcgt
     6421 cagcaggtcg atcaccgctt ccgtatgccc cgcccgccgg agcacccccc cctccgccgc
     6481 cttcagcgga aagatatgcc ccggccgccc gaagtcctgc ggcctcgaac ctggatcggc
     6541 cagcgccttg atcgtggccg cccggtcgtg cgccgaaata ccggtcgtcg tgccgatcag
     6601 gtaatccacc gagacggtga acgccgtgcc gtgcggcgcc gtgttcgtct ccaccatcat
     6661 gtcgaggccg agacgctcca ggccggcctc ggtcatcggc acgcagatca gcccgcgcgc
     6721 gaagcgggcg atgaagttca ccgcctccgg cgtgatcttc tcggcggcca tgatgacgtc
     6781 gccttcgttc tcccgatcgt catcgtccat gacgatgacc atcttcccgg cgacgagatc
     6841 ggcgatggcc tcgtcgatcg tggcgaacgt ggtcgtttcg gtgctcatga gcgtccttcc
     6901 gatgggagca ggcgttccag gtagcgcgct accgtgtcga cttcgatgtt gaccggccgg
     6961 ccgggctggt actcgccgca gatcgtttcc ttcagcgtgt gcgggatgat ggacatcgtg
     7021 attcgtgcac cgtcgggtgc gtcttcgagc gccgcgacgg tgagactgac gccgtcgatg
     7081 gcgatcgagc cgcgccgcgc gacgtaacgg ctgagttcgt tcggcacgtt gatagtgagc
     7141 cagaccgctt cgccgtcggg cttgatggcc acgaccgtgc cgacgccgtc cacgtggccg
     7201 gccaccaggt gcccgccgag ttcatcgccc gcgcggaggc tgggttcgag attcacgcgg
     7261 gcgccttcgc gaagcaagtc gcgcccgaag gtggtggttg tcatcgtggc ccccactgcc
     7321 tggaccgtgg cgtgtggggg gtcgagcttc gtgatcgtga ggcacacgcc gttcacggcc
     7381 agcgaggcgc cgatggacaa gcgcgccgcg gtgcgcggcg ctcgaattat caattcgaca
     7441 ccgttgggta tttcggtggc gcgctcgatg cgccccactt cggtaatgag cccggtgaac
     7501 acttacagat cccccgtgac gagccagtcg gtgccgatcg ggcgcagtgc cacgttcctg
     7561 aggcgcggcg cccggtcgac ccgggtcacg ccgagcccgt tggtccagcg gcgcgaatcg
     7621 ccgccgagga ggaccggcgc cgtgacgagc gccagccggt cggcctgttt cccggtcagg
     7681 aactgtcccg ccagtcggcc ccccccttcg acgagcaccg aggtgatccc ctcgcgggcc
     7741 gcgcgcttga gcagcatgga gagttggatg ccggaacggc cgtggcgatc cggacggaca
     7801 gccatcggcc agacctcggc gccggtggcc gcgaaggccc tttgccgcga ggcggccgcg
     7861 gccggcccgc agacaacgat ccggcggatg ccgtcgtcgg ccagccaggc ggccctcggt
     7921 gacgagcg
//
LOCUS       DASVOD010000419        13069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_133351, whole genome shotgun sequence.
ACCESSION   DASVOD010000419 DASVOD010000000
VERSION     DASVOD010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13069
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133351"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..705
                     /locus_tag="VF720_09630"
     CDS             142..705
                     /locus_tag="VF720_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7879658.1"
                     /translation="MKRDGDAELIQKALGGDQAAYRQLLEKYRRQVLSLVWRMVSNRE
                     EAQDLAQEAFIRAFRSLHTFDVTRSFPAWLFRITTNLCVDHYRRKKLDTVSLVIESDG
                     EERTRELESPDAAPDEEYRDKSESERLDRMVRALPAAYRIVILLRHQSDMSYEEVAEA
                     LNLPIGTVKARIHRAHKMLREKIERWG"
     gene            708..1361
                     /locus_tag="VF720_09635"
     CDS             708..1361
                     /locus_tag="VF720_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7879659.1"
                     /translation="MTTRNATGNRTCGWTEEHLFLWFDGDLPADERLRFERHLPGCAG
                     CEHDREAYANLFARLAALPAPVVGPAFDHGIMRGALRPAPVRFADPGNAWLRGMLLAA
                     AAAVVLVLGTLLLVDEQAVVNKAARPVAHLGARAAVDAVVQLMNTISLSQVVIDLVHA
                     LEPVGRTLGVIARAFQSEILLVSLLMSVVALLGAVRLADGTSAVERGVRRVCLALQI"
     gene            1336..2544
                     /locus_tag="VF720_09640"
     CDS             1336..2544
                     /locus_tag="VF720_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879660.1"
                     /translation="MSAWLSRSSVLTVMLSCLALAGGVGDSFGAVKPKTRHAQLDSLR
                     QAAEAAQRAVIEAERQAVEAETIVVATRRPSRTMSVDLGRHGKMTVNTDQSHGDKVLM
                     GDDLVVEADEVIGGDAVSMGGNITVLGKVMGSVVSVGGDVTLGDSSFVGKDAVSVGGE
                     VIRSETAIVGREVMEVDGPAIFKPRFGGRSHDDDARDSSFGHRFAGLVQTVIFFLVLF
                     AFAALALYLVRQRIEYASDYLSREPIPALLLGLVSPFLLLVSFVLLCITLIGIPVALA
                     LLVLYPVFIFVGWVVTGHRIGRSVAAGETPLRTVFAGLMVLAGLPLLFAFLRMVGITG
                     FLPGIVIFTGIMISSLGAFIGLGAILGTRFRRGPAGPTAPPDMVITSAPSAPPPAPPP
                     PSMTPPPAIS"
     gene            2846..3862
                     /locus_tag="VF720_09645"
     CDS             2846..3862
                     /locus_tag="VF720_09645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7879661.1"
                     /translation="MTTITTRTTSLAIIALVGGAFLASMAEASHGCRIHASDDVMATR
                     VRSTDGGLEFTDLSGHEWELVTSTTDPAISNAGSGSFHAATGSVVEGALAAVAWPGMS
                     RFDADIFLLPYPRRELLPSSAGKGAIYVSPGVHPWSTGPLHALVVHEMGHLVHERLLP
                     DTDVEGWKRYRQIRGITDESVYRAGAAHRNRPHEIFAEDFRALFGGPDANYSHSIENP
                     DLVPPDQVPGLYEFMAALAGQAGVTAASPAVPVLTAAPNPFHDATVLSIGAPVDANGA
                     FDVAIVDVSGRLVVGLGSFEAGTDKVGWNGRDAAGRAATAGVYYAVVTTGRARGVTRL
                     VLVR"
     gene            complement(3864..4544)
                     /locus_tag="VF720_09650"
     CDS             complement(3864..4544)
                     /locus_tag="VF720_09650"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX7879662.1"
                     /translation="MTPRGSALALAILALGVYSRPSQAAFNLAIGPVSSDSALVRLDF
                     RLDDPLPDPEADGSLVSPPAALAYTIELWRDRSTWFDALVSQRTYGYRLEVDRVRRVY
                     RVAVPDSGVIETADREELVRLLTEQVAVAAARLDDLSPGKHYYLAVTARLTPIDLDKL
                     EDVNAWLSGDIRPRNKGGVLGVPKSVVGFLADVAGLGDKAAISRSARFHREVVAGASR
                     LVIEVEDE"
     gene            complement(4586..5770)
                     /locus_tag="VF720_09655"
     CDS             complement(4586..5770)
                     /locus_tag="VF720_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEX7879663.1"
                     /translation="MRRTGWAALLVMMLTAGASGAEGNNDELSPGAALPVVRWGDLDR
                     DEPNQLTARMAYRRVDGAALYAGYRYHDATRRRPQLGVEIGYSIEREAGLWSLELAQP
                     LMSQERLMIGAGGWHATRHFDYDDEIVGSVENTLAALFFEDDYRDWYDGTGGRVFAAW
                     RWSRHWTVDAGLVVQDEEPLENRTDWSVFGGSDDFRENPAAARGTLHGTSIAFTHDTR
                     EKPRREGIPFIGEVDRSNHDVVSLSWHGAGGPLGGDFDFGLLRLDARGFVKLAPSQFL
                     AARIVAATATGDSLPPQENLFAGGVGTLPGHAHKRDRGDRLLLGNVEYSIDVWNAIQA
                     LVFFDVGRAWFDDLHPNGPKFHTDGGLGVQTRDQHLRLLLARDLADDGGLIFSIRTNA
                     TF"
     gene            complement(5767..7161)
                     /locus_tag="VF720_09660"
     CDS             complement(5767..7161)
                     /locus_tag="VF720_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7879664.1"
                     /translation="MNAMRSAPTVLIVDDEARVRKSLAAFLADEGYGVLEAGDLAEAR
                     AELEKAGDDLSLVLLDVWMPGGDGLDFLENGRDLLAGRPVVVMSGHGNIDMAVRAIRL
                     GAWDFLEKPVTPERLTVTLGRALEMARLRRERDDLRSDVAGGSPLLGSSAVMERLRKQ
                     IARVGPSSGKVLILGENGTGKELVARAIHEASPRSERAFVRVNSAAIPRDLIESELFG
                     HEKGAFTGATAARKGKMELADGGTLFLDEIGDMSLEAQAKLLRALENGEIERLGGSRT
                     IVVDVRLIAATNKNLKFEIQSGRFREDLYYRLNVVPIQVPPLREHMDDIPVLVAGFIA
                     RGLTGAGRPNFSLSSDAMSRLCDYPWPGNVRELRNAMERLSILAENDVVDAATLVRLL
                     PELTDNGEVSIHTGSDETNLRGAVEQAERRAIKEAIEAAGGNMSEAARRLGIDRANLY
                     RKMKRYQMDKGEGE"
     gene            complement(7158..8720)
                     /locus_tag="VF720_09665"
     CDS             complement(7158..8720)
                     /locus_tag="VF720_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7879665.1"
                     /translation="MAGDRHGTLRFRLQLAFLALTLIPGVALTLVLLQTLPWAFDQWA
                     SPSVSKTFDNALLVSRETLTRIENDLRQRWSLSVELAKPALWGGVDRFAPAIGERLNL
                     DFMVLYPPSPDSAWTPVRVLTRDALVEPPVGLRPWGPENPETGFFLRGERGELAFAQR
                     MPRESGPAGPVLAVGIYLDPTLYERLADLNTAMDRHDQVRYIGNVYKKGVQIGAVLLL
                     LVLGVGGVWIANRLADSLSSPVEDLAHARDRVAHGDLETRVTPRGTREIRSLVSAFNQ
                     MTTELGETREHLARAERAQAWRDAARRVAHEIRNPLQPITMALHRIDRSLADDPAHHA
                     EVQPAVHSILEEVAALRRLAASFSELARMPEPIPEPIDLAAFLRGSAPLLEFPGVALE
                     IDSRSAGPMAMADRGLLREVVTNLVKNASEAMPGGGRVAIHAGTASWSGRDWAVIDVE
                     DSGPGVPEDQRRRVFEPHVTTKAEGSGLGLAIVDRIIGAHGGRIEVDKGSLGGARFRV
                     WLPGVTREDTAR"
     gene            complement(8737..9804)
                     /gene="rlmN"
                     /locus_tag="VF720_09670"
     CDS             complement(8737..9804)
                     /gene="rlmN"
                     /locus_tag="VF720_09670"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX7879666.1"
                     /translation="MLRSGAMDTSPALRPILDRTLPELRSELVELGIEGWRADQIYTW
                     VYRKGVADFEAMTDIARATRERLAATYRIDVLVPKDERTASDRSATKCLFPLADGREI
                     EGVFLRLPTKETICFSTQVGCAIGCGFCVTALMGRLRNLAPGEIVGQILHLSRRFVER
                     EQGFNLVAMGMGEPFDNYDNLLTAVRIMKEVRGLNIGPRRITISTSGLVPMIDRLATE
                     GIPLGLAVSLNATTDEQRSEIMPINRKYPIEELLAAAGRYAKATSRRVTLEYVLLKGV
                     NDSEADARRLIKLSSRLPSKINLIPFNPSPYHSYAPPSPESAERFRQILIAADKTATI
                     RRRRGGDIYAACGQLGVTQAR"
     gene            9891..10319
                     /locus_tag="VF720_09675"
     CDS             9891..10319
                     /locus_tag="VF720_09675"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7879667.1"
                     /translation="MSPHHEVSQLFQALSDPTRLEILAIVATSEACASDIHTRLGMSQ
                     PRVARHLKILVSTGLLAARRDGRFVRYAISEDSSRARLVRAALGLIPPRPAAGERIAQ
                     AVQGGLPAAHQSLAAPAPAAGLETIAEVEPPRPPMEDFLL"
     gene            10316..11260
                     /locus_tag="VF720_09680"
     CDS             10316..11260
                     /locus_tag="VF720_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879668.1"
                     /translation="MTPSGPTAVIDVGTNTVRLLVAELDAGHPHVLAEFGEVTSLGTA
                     LVSTGRIDPTDAERTGTCLAEAVTRARGAGVPDPDVVGTEVFRKAGNGREVAEWLGAR
                     CGATLRILTPAEEADGSYLGAVAWGNPTMTGDLAVLDIGGGSTELIRGHGMSRQGSMS
                     LPIGALVATERWLKADPPGQTGLPEIRRALAAELSPLAGLTGMGRGAAVTVLGVGGSA
                     CSVAAWIGGIRPYVATRVHGRSIERSRLAGAIDEMADLTVAERAERAGIGLGRARVIL
                     GGTLVLEAALFAIDADAVVASTFGLRHGLIRKAWQPDL"
     gene            11512..11847
                     /locus_tag="VF720_09685"
     CDS             11512..11847
                     /locus_tag="VF720_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879669.1"
                     /translation="MHPKRALKLTLLLGFSLWALTALLPDREVLAANKKPGPAIEQAG
                     GSSPGAIIVSSRVNTGGTLLTGDPDQTTQSGDTQPSPAGVPGGRSLLERLLRTLGSWV
                     APIRETTVR"
     gene            11963..>13069
                     /locus_tag="VF720_09690"
     CDS             11963..>13069
                     /locus_tag="VF720_09690"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879670.1"
                     /translation="MRSKPRPSSSGHTGGDEYVRAVDRGDFLFRTENYSSALESYAQA
                     QALLDGRAGNDERGVLLLKVARCHQARGNMQQAIECLDEAKVDLRSSRDGVQLGKLYT
                     LRATLLYELGRYSRALRYADLAYKLLRGTGENSALAQLELTLGSLWTRLGDPEQAGQF
                     YTSALSTYRRIDDKVGTAKALNNLGILHKNACEWREAIRCFTGALELGEKQALIRFSV
                     AVRLNLGIVHFKLGDWNLAEESLKRARQIALENSFIAQLVPIDLALGNLERRRRRWNV
                     AAELYRGALERAEANDYSRETVLALEFTGELHLDREEFDAAAQFLIRALGRAEQIAPM
                     GDLVNEICRRLGEARLGQEQYEEALALGQKALSSS"
BASE COUNT         2092 a         4520 c         4242 g         2215 t
ORIGIN      
        1 acttgtatcc ccaatttgct tgaatgtcaa ggatttagga ggattcgcca ccttgtgccg
       61 cccggggtgg gagcgaggta atgaaacccc caatccgggc gtcccgtagt tgacctgcga
      121 gtcggaaggg cccaccggac ggtgaagcga gatggcgatg ccgagctgat ccagaaggcc
      181 cttggcggag accaggcggc gtaccgacag ctcctggaga agtaccgccg gcaggtgctg
      241 agcctggtgt ggcgtatggt gagcaaccgg gaagaggccc aggacctggc gcaggaagcg
      301 ttcatccggg cctttcgaag tcttcacacc ttcgatgtca cccggagctt cccggcctgg
      361 ctcttccgga tcaccacgaa cctgtgtgtg gatcactatc gccggaagaa gctcgatacg
      421 gtatcactgg tcatcgagtc ggacggcgag gagcggacga gggagctgga aagtcccgat
      481 gccgcgccgg acgaagagta ccgggacaag tcggagtcgg agcggctcga caggatggtg
      541 agggccttgc ccgccgccta ccgaatcgtg attcttctcc gccaccagag tgacatgtcc
      601 tacgaggaag tcgcggaggc attgaacctc ccgatcggaa cggtcaaggc ccggatccac
      661 cgggcgcaca agatgctgcg ggaaaagatc gagcgctggg gttgacgatg acgacacgga
      721 acgcaacagg caaccggacg tgcggctgga ccgaggaaca cctgttcctc tggttcgacg
      781 gcgacctgcc ggccgacgag cgcctccgct tcgaacgaca cctccccggc tgtgcgggtt
      841 gcgagcacga ccgtgaagcg tatgccaacc tcttcgcccg gctggcggcc ctgccggccc
      901 cggtcgtcgg accggccttc gatcacggca tcatgcgggg agccctgcgg ccggctccgg
      961 tccggttcgc cgatccgggc aacgcgtggc ttcgcggcat gctcctggcg gccgccgcgg
     1021 ccgtggttct cgtcctcggc acgctgctcc tcgtggacga gcaggccgtg gtgaacaagg
     1081 cagcccggcc cgtggctcac ctgggtgcgc gcgcggccgt cgatgccgtc gtccagctca
     1141 tgaataccat cagcctctcc caggtcgtca tcgacctggt gcatgcgctc gaaccggtgg
     1201 gacgcaccct gggcgtcatt gcccgcgcgt tccagtccga aatcctgctc gtcagcctgc
     1261 tgatgtcggt ggtggccctc ctgggcgccg ttcgtctggc cgacggaacg tctgcggtgg
     1321 aaaggggggt gcgccgtgtc tgcctggctc tccagatcta gtgtcctgac cgtgatgctg
     1381 tcgtgcctgg ccctggccgg cggcgttggt gacagcttcg gggccgtcaa gccgaagacc
     1441 cgccatgccc agctcgattc gctccggcag gccgccgaag ccgcccagcg tgcggtgatc
     1501 gaggccgaac ggcaggccgt cgaggccgaa accatcgtgg tggccacgag gaggccgagc
     1561 cgcaccatgt cggtcgatct cggccgccac ggcaagatga ccgtcaacac cgaccagagc
     1621 cacggcgaca aggtgctgat gggggacgac ctcgtcgtgg aagccgacga ggtgatcggt
     1681 ggcgacgcgg tgtcgatggg gggcaacatc accgtcctcg ggaaggtgat gggctccgtc
     1741 gtctccgtcg gcggcgatgt cacgctcggc gacagctcgt tcgtcggcaa agacgccgtc
     1801 tccgtgggtg gggaagtgat ccgctccgag acggccatcg tggggcgcga agtcatggag
     1861 gtcgatgggc cggccatctt caagccccgt ttcggcggca ggtcgcatga cgacgacgcc
     1921 agggacagtt ccttcggtca tcgtttcgcg gggctcgtcc agaccgtcat cttcttcctg
     1981 gtcctcttcg cgttcgcggc cctggccctc tacctggtcc ggcagcggat cgagtacgcc
     2041 agtgactatc tctcccgcga gccaattccg gcgctgctcc tcggcctggt ctcgccgttc
     2101 ctcctgctgg tgagcttcgt cctcctgtgc atcacgctca tcggcatccc ggtggcgctg
     2161 gcgctcctgg ttctctaccc ggtcttcatc ttcgttggct gggtcgtcac cgggcatcgg
     2221 atcgggcgct cggtggccgc cggagaaact ccgctgcgga ccgtgttcgc gggactcatg
     2281 gtcctggccg gcctgccgct cctcttcgcg tttctccgga tggtcggcat caccgggttc
     2341 ctcccgggca tcgtgatctt caccggcatc atgatctcaa gcctcggcgc cttcatcggg
     2401 ctcggcgcca tcctcggcac gcgtttccgg cgcggaccgg ccgggccaac ggcaccgccc
     2461 gacatggtga tcacgagcgc accttccgcg ccgccgccgg cgccgccccc cccgagcatg
     2521 acgcctcctc ccgccatttc ctgacgggcc atttaccccg atccccgacg aaggtcggga
     2581 atcggggcaa gtcgttggga cacatccgtt cgactccgcc tcacacgtcg atcccttgat
     2641 ttcacgggtg atcccacccg aaagcgtgtc gagccggtcc tcgtcggcgc cactccgctg
     2701 gtaatcacgt gcgcgcgtat tattcgctca tccctcacct gaccaatcac ttagacttga
     2761 tctcctcact tctcgtacat gccgcgtcat tgcagatcga gtggcacgcc gattgcggtt
     2821 tgccacgcca cggaggatca catcggtgac gaccatcacg acgcgcacga catctctcgc
     2881 catcatcgcc ctggtcgggg gcgccttcct cgcgtcgatg gctgaggcca gccacggctg
     2941 ccgcatccat gcctccgatg acgtcatggc cacccgcgtc cgctcgacgg acggcggcct
     3001 cgaattcacc gacctctccg ggcacgagtg ggagctcgtc acctcgacca ccgatcctgc
     3061 catcagcaac gccggcagcg gcagcttcca cgccgccacc ggctccgtcg tcgagggcgc
     3121 gctggccgcc gtggcctggc cgggcatgag ccgcttcgac gccgacatct ttctcctgcc
     3181 gtatccgcgc cgcgagctcc tcccgtcgtc ggccggcaag ggtgccatct acgtaagccc
     3241 cggcgtgcac ccctggtcca ccgggccgct gcacgcgctc gtcgtgcacg agatgggcca
     3301 cctcgtgcac gagcggctcc ttcccgatac cgacgtggag gggtggaaac ggtatcgcca
     3361 gatccgcggg atcaccgacg agagcgtcta tcgtgcgggc gcggcccatc gcaatcgccc
     3421 gcacgagatc ttcgccgagg acttccgcgc cctcttcggc gggcccgatg ccaattacag
     3481 ccacagcatc gagaaccccg acctcgtgcc gccggaccag gtgccggggc tctacgaatt
     3541 catggctgcg ctcgccggcc aggccggtgt caccgcggca tcgcccgcgg taccggtgct
     3601 gacggccgcg cccaatcctt tccacgacgc caccgtcctc tcgatcggcg cgccggtcga
     3661 cgcgaacggt gcgttcgacg ttgctatcgt ggatgtctcg ggacggctgg tcgtcggcct
     3721 cggctcgttc gaagcgggca ccgacaaggt ggggtggaac ggccgggacg cggccggccg
     3781 ggcggccacg gccggcgtgt actacgcggt cgtcacgacc ggcagggccc gcggcgtcac
     3841 gcgactcgtc ctggtccgct gactcactcg tcctcgacct cgatcaccag ccggctcgcc
     3901 ccggccacga cttcgcgatg gaaccgcgcg ctccggctga tggccgcctt gtcaccgagc
     3961 cccgccacgt ccgccaggaa tccgacgacc gacttcggca cgcccagcac gccacctttg
     4021 ttgcgcggcc ggatgtcgcc cgacagccac gcgttgacgt cctccagctt gtcgaggtcg
     4081 atgggcgtca accgggcagt gacggccagg tagtagtgct tgccgggcga caggtcgtcg
     4141 agccgtgccg ccgccacggc cacctgctcg gtcaacagcc gcaccagttc ctcccggtcg
     4201 gccgtctcga tcacgccgct atccggcacc gccacccggt agacgcgccg gacccggtcc
     4261 acctcgagtc gataaccgta cgtgcgctgc gacaccaggg catcgaacca ggtcgaccga
     4321 tcgcgccaga gttcgatggt gtaggcaaga gcggccggcg gcgaaaccag gctcccgtcg
     4381 gcctccggat ccggcagtgg atcgtcgagc cggaagtcga gtcgcacgag cgcgctgtcc
     4441 gagctgacgg gaccgatggc caggttgaag gccgcctgac tgggccggga gtaaacgccg
     4501 agcgcaagga tcgccagcgc gagcgccgat ccgcgcggag tcacgcgggc cgtgagcgga
     4561 gtccggcgtg gcaggacctg tccgttcaaa acgtggcgtt ggtccggata ctgaagatga
     4621 ggcccccgtc gtccgccagg tcgcgcgcca acaggagtcg caggtgctgg tcccgggtct
     4681 gcacgcccag gcctccatca gtgtgaaatt tcggcccatt cggatgcagg tcatcgaacc
     4741 aggcgcggcc gacgtcgaag aacaccaagg cctggatcgc gttccagaca tcgatcgagt
     4801 actccacatt gcccagcagg agcctgtcgc cgcggtcgcg cttgtgggca tgccccggca
     4861 gcgtccccac gccgcccgca aaaaggtttt cctgcggcgg gagtgaatcg ccggtggcgg
     4921 tggccgccac gatccgggcc gccaggaact gactgggcgc cagtttcacg aagccgcgcg
     4981 catcgagccg cagcagcccg aaatcgaaat cgcccccgag cggaccgccg gcaccgtgcc
     5041 acgagaggct caccacgtcg tggttcgaac gatccacctc gccgatgaac gggatccctt
     5101 cgcggcgcgg cttctcgcgc gtgtcgtgcg tgaacgcgat ggaggtgcca tgcagcgtgc
     5161 cgcgcgccgc cgccgggttt tcgcgaaagt cgtcgctgcc gccgaacacc gaccagtcgg
     5221 tccggttttc cagcggctcc tcatcctgca ccacaaggcc ggcgtccacc gtccagtggc
     5281 gcgaccagcg ccaggcggca aacacccggc ccccggtgcc gtcgtaccag tcgcggtaat
     5341 catcctcgaa gaagagcgcg gccagcgtgt tttccaccga gccgacgatc tcgtcgtcgt
     5401 aatcgaagtg acgggtggca tgccagccgc ccgcgccgat catcagccgc tcctggctca
     5461 tcagcggctg tgccagctcc agggaccaga gtccggcctc gcgctcgatc gaataaccga
     5521 tctccacgcc aagctgcggc cgtcgccgcg tggcgtcgtg atagcggtag ccggcgtaca
     5581 acgccgcgcc atccacccgc cgataggcca tccgtgcggt gagctggttc ggctcgtccc
     5641 gatcgagatc gccccagcgc accaccggca gcgccgcccc gggggagagt tcgtcgttgt
     5701 ttccctcggc ccccgaagcg ccggccgtga gcatcatgac caggagtgcc gcccaacccg
     5761 tccgcctcac tcgccctccc ccttgtccat ctggtagcgc ttcatcttgc ggtagaggtt
     5821 ggcgcggtcg atgccaagtc gccttgccgc ctcggacatg ttgccgcccg ccgcttcgat
     5881 ggcctccttg atcgcccggc gctcggcctg ctccacggcg ccccggaggt tggtctcgtc
     5941 gcttccggta tggatcgaaa cctcaccgtt atccgtcaat tccgggagga gccggaccag
     6001 cgttgccgca tccaccacgt cattttccgc caggatggaa agtcgttcca tggcgttgcg
     6061 gagctcgcgg acattccccg gccacggata gtcacagagg cggctcatcg cgtcggaact
     6121 caacgaaaag ttcgggcgcc ccgcaccggt caacccccgg gcgatgaagc cggccaccag
     6181 caccgggatg tcgtccatgt gctcgcgaag cggaggcacc tggatcggca ccacgttgag
     6241 ccggtagtag aggtcttccc gaaaccgccc ggactggatc tcgaacttga ggttcttgtt
     6301 ggtggcggcg atcagacgaa catccaccac gatggttcgt gatccgccca gccgttcgat
     6361 ctccccgttc tcgagcgcgc gcaggagctt ggcctgcgcc tcgagactca tgtcgccgat
     6421 ctcatcgagg aagagggtgc cgccgtcggc cagctccatc tttcccttcc gggccgcggt
     6481 ggcgccggtg aaggcgcctt tctcgtgccc gaacagttcg ctttcgatga ggtccctggg
     6541 gatggccgcg ctgttcaccc gcacgaacgc ccgttccgat cgcgggcttg cctcgtggat
     6601 ggcccgggcc accagttcct ttccggttcc gttttccccg aggatcagga cctttccgga
     6661 actcggtccc acgcgggcga tctgcttgcg cagacgttcc atgaccgcac tcgatccgag
     6721 caacggcgaa ccgccggcca catcgctcct cagatcatcc cgctcccttc tcaggcgggc
     6781 catctcgagc gctcgcccca gggtcacggt gaggcgctcc ggtgtcaccg gcttctcgag
     6841 gaaatcccag gcgcccaacc ggatggcgcg aaccgccatg tcgatgttgc cgtgcccgct
     6901 catcacgacc acggggcgac cggccagcag gtcacgtccg ttttcgagga agtcgagtcc
     6961 gtcgccgccc ggcatccaga catcgaggag cacgagcgag aggtcgtcgc cggctttctc
     7021 gagctcagcg cgggcctcgg ccaggtcacc ggcttcgagc acgccgtatc cctcatcggc
     7081 aagaaaagcc gcgagcgatt tccggacgcg cgcctcgtca tcgacgatga gcacggtcgg
     7141 agcgctgcgc atggcattca tcgcgcggtg tcctcccgtg tcacgcccgg caaccagacg
     7201 cgaaagcggg cgcccccgag gctcccctta tccacttcga tccggcctcc atgtgcgccg
     7261 atgatccggt cgacgattgc caggccgagc ccggaacctt ccgccttggt cgtgacgtga
     7321 ggctcgaaga cgcgtcgccg ctgatcttcc ggaactcccg gtcccgaatc ctcgacgtcg
     7381 atgacggccc agtcgcggcc gctccaggat gccgtgccgg cgtggatcgc cacccggcca
     7441 ccgcccggca tggcctcgct cgcattcttc accaggttgg tcacgacttc gcgcaagagc
     7501 ccccggtcgg ccatggccat cggtcccgcc gaccggctgt cgatctcgag cgcaacgccg
     7561 gggaattcga ggagcggcgc gcttccccga aggaacgcgg ccaggtcgat cggctcgggg
     7621 atcggttcgg gcatccgggc cagttcggaa aagctggcgg ccagccggcg gagcgcggcc
     7681 acctcctcga ggatcgagtg aaccgccggc tgcacctcgg catggtgggc cggatcgtcc
     7741 gccagcgacc tgtcgatccg gtggagcgcc atggtgatcg gctggagggg gttccggatc
     7801 tcgtgtgcca cccgtcgcgc cgcatcgcgc cacgcctgcg cgcgctccgc gcgggccagg
     7861 tgttcgcgcg tctcgccgag ctcggtggtc atctggttga acgcggacac gaggctcctg
     7921 atttcgcggg tgccgcgcgg ggtgacgcgc gtttcgagat caccgtgcgc cacgcgatcc
     7981 ctcgcgtggg caagatcttc caccggactg gaaaggctgt cggccagtcg attggcgatc
     8041 cacacccccc cgacgccgag gaccaggaga agcagcaccg ccccgatctg gacgcccttc
     8101 ttgtacacat tgccgatgta gcgaacctga tcatgccgat ccatcgccgt attgagatca
     8161 gcgagtctct catacagcgt gggatcgagg tagatgccca cggcgagcac ggggccggcg
     8221 ggtcccgact cccgcggcat gcgctgcgcg aaggccagtt cgccccgctc gccacgcagg
     8281 aaaaatcccg tttccgggtt ttccggcccc cagggtcgca ggcctaccgg aggctcgacc
     8341 aacgcatcgc gcgtgagcac gcggaccggt gtccaggccg agtcgggaga ggggggatag
     8401 agcaccatga aatcgaggtt gagtcgttcg ccgattgcgg gcgcgaagcg atccaccccg
     8461 ccccacagcg ccggcttggc cagttccacc gacaacgacc agcgctggcg caggtcgttc
     8521 tcgatgcggg tcagcgtttc ccggctcacc aggagagcgt tgtcgaacgt cttgctcacg
     8581 ctcgggctgg cccattgatc gaacgcccat gggagggtct gcagcagcac gagtgtcagc
     8641 gccacgcccg ggatcagggt cagtgcgagg aaggcgagct gaagccggaa gcggagggtg
     8701 ccgtgccggt ccccggccac tcgtatggtg ccgggcctac cgggcctgcg tgaccccgag
     8761 ctgtccacag gcggcgtaga tgtcaccgcc gcggcgccgg cggatcgtgg ccgtcttgtc
     8821 ggccgcaatc aggatctggc ggaagcgttc cgccgactcg ggagaggggg gcgcatagct
     8881 gtggtagggg gatgggttga acgggatgag gttgatcttc gacggaaggc gcgaggagag
     8941 cttgatcaac cgtcgcgcgt cggcctcgga atcattgacc cctttgagca gcacgtattc
     9001 gagcgtgacg cgtcgactgg tggccttcgc gtaacgcccg gccgccgcga gcaattcttc
     9061 gatcggatac ttccggttga tcggcatgat ctccgagcgc tgttcgtcgg tggtggcatt
     9121 gagcgaaacg gccagcccaa gcgggattcc ctcggtggcc agccggtcga tcatggggac
     9181 gagcccgctg gtcgaaatcg tgatgcgacg ggggccgatg ttgagcccgc gaacctcctt
     9241 catgatccgc acggcggtga ggagattgtc gtagttgtcg aacggttcgc ccatacccat
     9301 ggccaccagg ttgaagccct gctcgcgctc cacgaatcgc cgcgagagat gcagtatctg
     9361 ccccacgatt tcgccgggcg ccaggttgcg caaccgaccc atcagggccg tgacgcagaa
     9421 cccgcatccg atcgcgcaac ccacctgcgt ggaaaaacag atcgtttcct tggtagggag
     9481 gcggaggaag actccctcga tctcgcgccc atccgccagt gggaagaggc acttggtggc
     9541 cgatcggtcc gaggcggttc gttcgtcctt cggaacgagg acgtcgatcc ggtaggtcgc
     9601 ggccagccgc tcgcgcgtgg cgcgagcaat gtcggtcatg gcctcgaaat cggccacacc
     9661 cttccgatag acccaggtgt agatctggtc ggcccgccac ccttcgatcc cgagctcgac
     9721 cagttcggag cgaagctcgg ggagggtgcg atccagaatc ggccggaggg cgggggaggt
     9781 atccatcgcg ccggatcgta gcatgtccgc acgccgggtc gaagcccgcg tccggaccat
     9841 ctccagaatg tgcttgacat ctatttggat atagatatgt ttatgtaact atgtcgcccc
     9901 atcacgaagt ctcccaactg tttcaggcgc tctccgaccc cacccggctc gagatcctgg
     9961 ccatcgtggc cacctccgaa gcatgcgcca gcgacatcca cacccggctc gggatgagcc
    10021 agccccgggt ggcgcgccat ctcaagatcc tcgtctccac cggtctgctc gcggctcgcc
    10081 gggacgggcg gttcgttcgt tacgcgatca gcgaggattc ctcccgagcc cggctcgtcc
    10141 gggcggcatt gggactgatc ccgccgcgtc cggcggccgg tgagcggatc gcccaggcag
    10201 tccagggtgg cctgccggcg gcacaccaga gcctcgcggc acccgcgccc gccgccggcc
    10261 tcgaaacgat cgccgaagtc gagcctccaa ggcccccgat ggaggacttc ctcctttgac
    10321 cccttccggg cccaccgccg tcatcgatgt cggaacgaac acggtccggt tgctggtggc
    10381 cgaactcgac gccggacacc cgcatgtcct ggctgaattc ggcgaggtca ccagcctggg
    10441 gacggccctg gtcagcacgg gccggatcga cccaacggat gcggaacgaa ccggtacttg
    10501 cctggccgaa gccgtaacgc gagcgcgggg cgccggtgtg cccgatcccg acgtcgtggg
    10561 aaccgaggtc tttcgcaagg ccggcaatgg tcgggaagtg gccgagtggc tgggcgcccg
    10621 ctgcggcgcc accttgagga tcctgactcc ggccgaggag gcggacgggt cgtatctcgg
    10681 cgctgtggcc tggggtaacc ccaccatgac aggggacctt gccgtgctcg acatcggtgg
    10741 tggttcgacg gagttgatcc gcggccacgg catgtcgcgc cagggatcga tgagcctgcc
    10801 gatcggcgca ctcgttgcca cggagcgctg gctgaaagcc gatccacccg gccagaccgg
    10861 gctgccggag atccgccggg cacttgctgc cgagctctca cccctggccg gactgaccgg
    10921 catgggtcgg ggcgccgcgg tgaccgtgct gggtgtcgga ggctccgcct gttcggtggc
    10981 ggcctggatc ggcggcatca ggccctacgt ggccacccgt gtgcacggtc gctccatcga
    11041 gcggagccgg ctcgctgggg cgatcgatga aatggccgac ctcaccgtcg cggagcgggc
    11101 cgagcgtgcc gggatcggtt tggggcgggc ccgcgtgatc ctgggcggca cgctggtgct
    11161 cgaggcggca ctttttgcca tcgacgccga cgccgtcgtc gccagcacgt tcgggcttcg
    11221 acacggtttg atccgaaaag catggcagcc cgacttgtga aaaaaatcac aagcgccgga
    11281 gctctaaatt cttttgacag ttgaggaaat ggctacctat actgcctgcg gggagcgtac
    11341 ctggatgccg gagcgacttt gctcgcgccg gctatcgccc gcctcccggc cgaaggcatg
    11401 acggggtccc ctctcctcgc aaaccgttcg gcaccaccat gtcgtacagg gcatcgggga
    11461 atccaaactg gtcgcgcccc ggtgcacgtt cggagtgaag gagctacctc catgcatccc
    11521 aagcgagccc taaaactcac cctgcttctc ggcttctcgc tttgggcgct gacagcactc
    11581 cttccggatc gtgaggttct ggccgcaaac aagaagccgg ggcccgcaat cgagcaggcc
    11641 ggtggttcgt ccccgggcgc catcatcgtg tcctcccgtg tcaacacggg cggtacgttg
    11701 ctgaccggcg atcccgatca gacgacgcag agtggcgaca cgcagccttc ccccgccggc
    11761 gttcccggcg gtcggagcct gctggagcgc ctgctgcgca cactgggcag ctgggtcgcg
    11821 ccgatccggg agaccactgt ccgctgacct gaaccgataa gtctcgaagc caagttcgcc
    11881 gtcacagtcg tgtcggtttt tgtagatcta tggtcgtcgg gggcgtcagg ccatggaaga
    11941 tttccagcag gaatcaggtc gcatgcgatc gaagccgcgg ccatcgtcgt ccggtcacac
    12001 gggcggggat gagtacgtcc gcgcagtcga tcggggcgac ttcctgttcc gaaccgagaa
    12061 ttacagctcg gcgctggaga gctatgccca ggcccaggcc ctcctcgatg ggcgcgccgg
    12121 caacgacgag cgcggtgtac tcctcctcaa ggtggcccgc tgtcaccagg cccgcggcaa
    12181 catgcagcag gccatcgagt gcctcgacga ggccaaggtc gatctccgca gcagccgtga
    12241 tggggtccag ctcggcaagc tttacacgct ccgcgccacc ctcctctacg aactcggccg
    12301 gtacagccgc gccctccgct atgccgatct cgcctacaag ctcctgcgtg gcaccgggga
    12361 gaactccgcc ctcgcgcagc tcgaactgac gctcggctcc ctctggaccc gccttggcga
    12421 tccggagcag gccggtcagt tttacacgag cgccctctcc acctaccgtc gcatcgacga
    12481 caaggtgggc accgccaagg cgctgaacaa cctcggcatc ctccacaaga acgcctgcga
    12541 gtggcgcgaa gccatccgct gcttcaccgg cgccctcgag ctcggcgaaa agcaggcgct
    12601 catccgcttc agcgtggccg tccgcctgaa cctcggcatc gtccacttca agctgggcga
    12661 ctggaacctg gccgaagaat cgctcaagcg ggcccgccag atcgcgttgg agaactcctt
    12721 tatcgcccag ctcgtgccga tcgacctggc gctgggcaac ctggagcgcc gccgccgtcg
    12781 ctggaacgtg gcggccgaac tgtaccgtgg agccctggaa cgggccgaag ccaacgacta
    12841 ttcgcgcgag accgtgctgg ccctcgagtt cacgggagag cttcacctcg accgcgagga
    12901 gttcgacgcc gccgctcagt tcctgattcg ggcgctcggt cgcgccgagc agatcgcccc
    12961 gatgggcgat ctcgtgaatg aaatctgccg ccggttgggc gaagcgcggc tcggccagga
    13021 acagtacgaa gaggcgctgg ccctcggcca gaaggcgctc tcctcctct
//
LOCUS       DASVOD010000420         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_498177, whole genome shotgun sequence.
ACCESSION   DASVOD010000420 DASVOD010000000
VERSION     DASVOD010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498177"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="VF720_09695"
     CDS             <1..1414
                     /locus_tag="VF720_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HEX7879671.1"
                     /translation="TAVRGGAGPLTVRFGIGWPGETAADRASLIHLVKRLKVEAGITA
                     GQRLIVDVSPYVPTPGSALSAAKGAEAFDAVRESEAVTGALTGEAGVSVRVVPAELAA
                     IESRLLMGGREIGARLVAAADRGAMTPDPIRPWDPATWIEAFAPSSGESRIDWWKGDW
                     SPTSGGGSVPSPPVVNDTPIVTTQPPSAALPLTADTAGYGRKSRRKAPASMRPASRYR
                     VRFAKTEALRYTSHLDVGRLVERALSRLERTRPAWYNRSVKLSFGPPLPLGWTGEDEF
                     VDLLFPEDVPETLPQALMEVLPAGIEMIDVKPIRASVESLSHAIDRARYSIPLRPEWL
                     DAGGAEPGSRLARLTESVARLKSAREILVRKSPDDEINRLDVRPGVLGAQVEMNARGE
                     ACLALHLTLGVPGSVRPEVLLAHLLGDDAETARLAPVHRAALRIAGNGKEFSPMEVVE
                     LDFPWWRESARRTAAGGPRA"
     gene            1407..>1815
                     /locus_tag="VF720_09700"
     CDS             1407..>1815
                     /locus_tag="VF720_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013835156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEX7879672.1"
                     /translation="MRRDIVVNADPNETRIAILEEKQLVEFLVERPSERRIVGDIYKG
                     RVNAVLPGMQAAFVDIGLARTAFLHASDLAEGIFQDEVDLDADDDSADNWLEDDPDEG
                     EEGEEEESNGDGESAEAPRGDRRGGRGRDARGRG"
BASE COUNT          274 a          653 c          629 g          259 t
ORIGIN      
        1 caccgctgtt cgcggcggtg ccggtcccct caccgtgcgc ttcggcatcg gttggcccgg
       61 cgaaacggcg gccgaccgcg cgtcgctcat ccacctggtc aagcgcctca aggtcgaagc
      121 cgggatcacg gccggccagc ggctgatcgt cgatgtgtcg ccctatgtgc ccacgcccgg
      181 ttccgcactg tccgccgcaa agggggccga ggctttcgac gcggtgcgcg agtcggaggc
      241 tgtgaccggg gcgctcaccg gcgaggccgg cgtgagcgtc cgcgtcgtgc cggccgaact
      301 cgccgccatc gaatcgcgcc tgctcatggg ggggcgcgaa atcggcgccc ggcttgtggc
      361 cgccgccgac cgcggcgcca tgacacccga ccccatccgc ccttgggatc cggccacctg
      421 gatcgaggcg ttcgcgccgt cgtcgggcga atcgcggatc gattggtgga agggcgattg
      481 gagtccaacc tcggggggag gttccgtgcc gtcgccgccc gtggtcaacg acacgccgat
      541 cgttaccacc caaccgccgt cggccgccct gccactcacg gccgacaccg ccggctacgg
      601 ccggaagtcg cgccgcaagg cgccggcttc gatgcgtccc gcctcgcgtt accgggtgcg
      661 cttcgccaag accgaagccc tgcgttacac ctcgcacctg gacgtggggc gcctcgtcga
      721 gcgcgcgctg agccgcctcg agcggacgcg tcccgcctgg tacaaccgct cggtgaagct
      781 gtcgttcggc ccgccgctgc cgctcggctg gaccggcgaa gacgaattcg tcgacctgct
      841 cttccccgag gacgtgcccg aaacgcttcc gcaggcgctg atggaggtgc ttcccgccgg
      901 gatcgagatg atcgacgtca agccgatccg cgcctccgtg gagagcctct cgcacgccat
      961 cgaccgcgcg cgctactcga ttccactgcg gccggagtgg ctggatgcgg ggggtgccga
     1021 accgggtagc cgactggccc gcttgacgga gtcggtggcc cgcctcaaga gcgcgcgcga
     1081 aatcctggtg cgcaagtcgc cggacgacga aatcaatcgc ctggatgtcc gcccgggggt
     1141 cctgggcgcg caggtcgaaa tgaacgcccg tggcgaggcc tgcctcgccc ttcacctgac
     1201 cctgggcgtg cccggaagcg tgcgcccaga agtgctcctg gctcatctcc tcggcgacga
     1261 cgccgaaacg gcgcgcctgg cgcccgtcca ccgcgcggcg cttcgcatcg ccggcaacgg
     1321 aaaggaattc tccccgatgg aggtggtaga gctcgatttc ccgtggtggc gcgagtcggc
     1381 gcggcgtacg gcggccggag gcccgcgtgc gtagagacat cgtcgtcaac gccgatccca
     1441 acgaaacccg aatcgccatc ctcgaggaaa agcagctcgt cgaattcctc gtggagcgcc
     1501 ccagcgaacg ccgcatcgtc ggcgacatct acaagggacg ggtcaacgcc gtcctccccg
     1561 gcatgcaggc ggccttcgtc gacatcgggc tggcccgcac cgccttcctc cacgcgtccg
     1621 acctggccga ggggatcttc caggacgagg tggacctcga cgccgacgac gactccgccg
     1681 acaactggct ggaggacgat ccggacgaag gcgaggaggg ggaggaagaa gagtcaaacg
     1741 gcgacggcga gtccgccgaa gccccccgtg gcgatcgccg cggcggtcgc ggtcgcgatg
     1801 cccgcggcag ggggc
//
LOCUS       DASVOD010000421         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_757910, whole genome shotgun sequence.
ACCESSION   DASVOD010000421 DASVOD010000000
VERSION     DASVOD010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757910"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VF720_09705"
     CDS             <1..522
                     /locus_tag="VF720_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879673.1"
                     /translation="VPRGDVSSSDPDAPGLDTLSTHLFLRGAEMVTTRRSGPLDIART
                     IRNGVVEPAFGIVRLDDRVVITNQDNVPRRLFWFDGQTTRISNPIPPGASVSRPARRF
                     GPITMATDVSGSPRSIAFVAENPFLATATPDGAFRMTGVPPGDYRVVAWAPAGYLRDW
                     VVRVPFEGSVAAE"
     gene            complement(584..913)
                     /locus_tag="VF720_09710"
     CDS             complement(584..913)
                     /locus_tag="VF720_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7879674.1"
                     /translation="MGTGVIALDASALLAWLFREPRWEIVDERLAEGAISTVNLAEVL
                     CRLRRDDFPSHRMRQALEAGPLDIVPSAPTMPNSSHRWNHSPGRTASPWEVAPAWLWR
                     CPTDVRH"
     gene            complement(898..1164)
                     /locus_tag="VF720_09715"
     CDS             complement(898..1164)
                     /locus_tag="VF720_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009819916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEX7879675.1"
                     /translation="MAVLNIRNLSDDVHQRLRERAERHQRSMEAEARTILTDVLLTER
                     RSSPSMEHVRELIDTFYGDQKPTGVVDEFLAERRREAEREWERE"
     gene            complement(1251..1814)
                     /locus_tag="VF720_09720"
     CDS             complement(1251..1814)
                     /locus_tag="VF720_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009582762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7879676.1"
                     /translation="MSTSNYRRDEAARLLEATPGLLDAWLRNLPESWLAANEGEGTWS
                     PIDIVGHLIHGEEADWIPRARHLLDHGESKPWEPFDRFGMFTKFGGWSMPRLLDRFAE
                     ARAESLVMLTSWQLAEADLDRSTLHPEFGVVTLRQHLATWVAHDLNHVAQIARVMAGR
                     YRGDAGPWSAYLPILKHRDQGSSKSNN"
     gene            complement(1814..>2769)
                     /locus_tag="VF720_09725"
     CDS             complement(1814..>2769)
                     /locus_tag="VF720_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7879677.1"
                     /translation="TNAHLSPEMGYGRGFDRYKCIGFTSADSVNRSVLRWKSVIGNGK
                     GPWFLWIHYYDPHHPYQEQRPWFREFVPDVTRADAPLIQSAKGQPPKAPQRGTKAADR
                     FVVLARGLYDAEVRHTDEAIRNLFREMPELGDAVIVMAGDHGEEFLEHGNTGHCRNLN
                     GETVRVPLFIRHPESGGAGRRNDPVSLVDMPATLLALGGGRWPVPSPGVSLLGPPSAG
                     RTLLAQLERNREYGLDEAVIGPRWKFILNRLSKKTQLYDIAADRAESRNLVQSNAADV
                     ETQRALLEALLKGLAPPPAVVEKRTLSKEVEAQLRGAGYIH"
BASE COUNT          440 a          903 c          924 g          502 t
ORIGIN      
        1 gtgccacgcg gcgatgtctc ctcatccgat cccgacgcgc ccggtctcga taccctctcc
       61 acccacctct ttctgcgcgg cgcggaaatg gtgacgaccc gtcgcagcgg tcctctcgac
      121 atcgcgcgca cgatccggaa cggagtcgtg gagcccgcct tcggtattgt ccggctcgac
      181 gatcgcgtcg tcattaccaa ccaggacaac gtgccgcgtc gcctcttctg gttcgatggc
      241 cagaccaccc gcatctcgaa cccgatcccg cccggcgctt cggtttcgcg tccggcacga
      301 cgattcgggc cgatcacgat ggccaccgac gtgtcgggat cgccgcgttc gatcgcgttc
      361 gttgccgaga atccgttcct ggccacggcc acgccggatg gtgcgttccg gatgacgggc
      421 gtgccgccgg gtgactatcg agtcgtggcc tgggccccgg cgggctatct gcgcgattgg
      481 gtggtgcgcg tgccgttcga ggggagcgtg gcggcggagt gacgtcagcg gatcaggatc
      541 acctggaagc cggcgggtag tcgcccccag ttccgatcgg cagtcaatgc cggacatccg
      601 ttggacaccg ccagagccag gcaggcgcga cctcccaggg agaggccgtg cgaccgggcg
      661 agtggttcca gcgatgcgac gagttcggca tcgtcggcgc ggatggcacg atgtcgagag
      721 gacctgcctc gagagcttgc cgcatccggt gtgacgggaa gtcgtcgcgg cgcaatcggc
      781 acaagacctc ggccaggttg acggtcgaga tggcaccctc ggcgaggcgt tcatcgacga
      841 tctcccagcg tggctcacgg aagagccagg caagaagcgc cgaggcgtcg agggcgatca
      901 ctcccgttcc cattctcttt ccgcctcgcg acggcgctcg gcaaggaatt catcgaccac
      961 tcccgtcggt ttctggtcgc cgtagaaggt gtcgatcagc tcccgcacgt gctccatgga
     1021 cggcgaggac ctcctctcgg tcagcaggac gtccgtcagg atcgtccgcg cttccgcctc
     1081 catgctccgt tggtgccttt ccgcccgttc gcgcaggcgc tggtggacgt cgtcggagag
     1141 attgcgaatg ttgagcaccg ccatgattcc tccgggggga ttccggttcg gggggttaat
     1201 tcggtaccca gtagttgtag gcaaaatgcc tacagagccg tcaagtcaaa tcagttattt
     1261 gacttgctgc tgccttggtc ccggtgcttg agtatcggca ggtaggccga ccacggaccg
     1321 gcgtcacccc ggtagcgccc ggccatcacc cgcgcgatct gggccacgtg attcaggtcg
     1381 tgcgctaccc aggtggcgag gtgctggcgg agcgtcacca cgccgaattc ggggtggagt
     1441 gtgctgcgat cgaggtcagc ttcggccagc tgccacgatg tcagcatcac aaggctctcg
     1501 gcccgcgcct ccgcgaagcg atccagcagt cgcggcatcg accagccgcc aaacttcgtg
     1561 aacatcccga aacgatcgaa cggctcccac ggtttcgatt cgccgtgatc gaggagatga
     1621 cgcgcgcggg ggatccagtc ggcctcctcg ccgtggatca agtgaccgac gatgtcgatg
     1681 gggctccagg tgccctcgcc ctcgttcgcg gcgagccacg attccggaag atttcgaagc
     1741 catgcgtcga gcaggcccgg cgtggcctcg agcaggcgag ccgcctcgtc ccgccggtag
     1801 ttcgaggtgg acatcagtgg atgtatcccg cgccgcgcaa ttgcgcctcc acctctttcg
     1861 acagggtccg cttctcgaca accgccggcg gcggcgccag acccttgagg agtgcctcga
     1921 ggagggcgcg ctgtgtttcc acatcggccg cgttcgactg gacaaggttt cgcgactcgg
     1981 cgcgatcggc cgcaatgtcg tagagctgcg tcttcttcga caggcggttc aggatgaact
     2041 tccaccgtgg gccgatgacg gcttcatcaa gcccgtactc gcggttgcgt tcgagctgcg
     2101 cgaggagtgt ccgccccgcg gacggcggac caagaagtga cacgcccggc gaggggacgg
     2161 gccaccggcc gcctcccagt gccagcaggg tggcaggcat gtcgacgagg cttaccggat
     2221 cattgcgtcg tccggcacct ccgctttcgg ggtgccggat gaagagcggc acccgcaccg
     2281 tctcgccgtt caggttgcgg cagtgacccg tgttgccatg ctcgaggaac tcttcgccat
     2341 gatcgccggc catcacgatg acggcatcgc cgagttcggg catttcccgg aagaggttgc
     2401 ggatggcctc gtcggtatgg cgcacctcgg cgtcgtacag cccccgggcc aggaccacga
     2461 aacggtcggc ggctttggtg ccgcgctgcg gtgccttcgg cggctgtccc ttggcggact
     2521 ggatcagggg ggcgtcggcg cgcgtcacgt ccggcacgaa ctcgcgaaac caggggcgct
     2581 gctcctggta ggggtgatgc gggtcgtagt agtgaatcca gaggaaccac gggcccttgc
     2641 cgttgccgat gaccgacttc cagcgcagca ccgaacgatt gacgctgtcg gccgacgtga
     2701 acccgatgca cttgtagcga tcgaatccgc gcccgtatcc catctccggc gagagatgcg
     2761 cattggtgg
//
LOCUS       DASVOD010000422         3503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_600981, whole genome shotgun sequence.
ACCESSION   DASVOD010000422 DASVOD010000000
VERSION     DASVOD010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600981"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..>3503)
                     /locus_tag="VF720_09730"
     CDS             complement(193..>3503)
                     /locus_tag="VF720_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HEX7879678.1"
                     /translation="TAEPIALDRPLTGTVGAVLDPIAAVRVRLHLPPGGFARLAFGTG
                     MADSAEAATLLANKYRDPAAATRAFSLAFARTQIELRHLGLSRDEAQLFQRLASVVLG
                     PDRSLRAAPEVHAANVKGQPDLWPFGISGDVPILLVRVVEENDLPLVRQVLKAQEYWH
                     LKNLSADVVILNEHAMDYRDEMHHQLETLLESGSWAGWRNRPGGVFLLRGDGMAEADH
                     TLLMSAARAVLKGDLGELADQVSMSPAEPEYPERFVPTRGRAAPAIAPDLPTLEPPPF
                     VLPHGLGGFTNDERPAAVVVLDDDRETPAPWINVLANAHFGTLVTTTGAAFTWSGNSR
                     QNRLTPFTNDPVSRPTGEAIWLRDEETGESWPVMPALEPPPLRRGRWIVRHEAGRSSF
                     RHAGRGLEHELELVVDPEDPVKHSVVSITNRGDAPRRLSLFAWHEWVMGPSRPDDHLH
                     VVTESVAELGAILAHNPWNRDFPGRVAFAALGEIPDSVTGDRAEFIGRNGTPSNPAAL
                     YRRALSGRTGAGMDPGAGLHRVLEIAPGETRRINCVLGEAADREAALALVRRHAGLGH
                     ADRVREATRLAWANRLDTLRVTTPDDSFDLMINRWLVYQNLACRVWARSGFYQPGGAF
                     GFRDQLQDVMALLHAAPEVAREHLVRAAGRQFVEGDVQHWWHPHDGRGMRTRCSDDLL
                     WLPWAAAQYVRTTGDASVLDTVLPFLEAPPLEPHEAESYRLPTTSGESGTLYEHCRRA
                     IDRGLTAGAHGLPLFGSGDWNDGMNRVGIEGKGESVWLGWFLHAVLSQFVPICQARGD
                     DEAAARYRREARRLADAMELAWDGKWYRRGWFDDGTPLGSSQNEECRIDSISQSWAVL
                     SGAAPPNRAESAMDAVRAQLVNRAHQLVLLLTPPFDKSPVDPGYIKGYVPGIRENGGQ
                     YTHAAIWMAMATARLGNGEESVELFHLLNPINHARTVADVDRYVTEPWAVAADVYAHP
                     MHAGRGGWTWYTGSAGWLYRLGVENILGLRRHGSEFELDPCIPSAWPNFTIDWRVGTT
                     RYRIQVDNPRGECRGIHEVRLDGRLVDARAIPLVEDGKEHEVVATLGARSDRPQSPRP
                     PGNAKPSPATRALA"
BASE COUNT          519 a         1164 c         1304 g          516 t
ORIGIN      
        1 ccccacccca cagctaaggg ctacccacta cttcctggcc tgccccgaat ccctgacggg
       61 ggggctcttg aatgggggct cctctatata ttgggggctg cgactgtctg gagcacggtg
      121 ggtgccggcg ccagggcggc ccgaagggcc gctggcgcgt atcgttatac gcctcaatat
      181 gttacagagg aattaggcta gggctcttgt ggctggggag ggcttggcat tgccgggggg
      241 gcgtggagat tgggggcggt cggatctggc gccgagggtg gctaccactt cgtgctcctt
      301 gccgtcctcc accaggggga tggcacgagc atcgaccaat cggccgtcga ggcggacctc
      361 atggatgccc cggcactctc cgcggggatt gtccacctgg atgcggtagc gggtggtgcc
      421 gacgcgccag tcgatcgtga agttgggcca ggcggagggg atgcaggggt cgagctcgaa
      481 ctcgctgccg tggcggcgga ggccgagtat gttctcgacg ccaaggcgat agagccagcc
      541 ggcggagccg gtgtaccagg tccagccacc ccggccggca tgcatggggt gggcgtagac
      601 gtcggcggcc acggcccatg gctcggtgac gtagcgatcg acgtcggcga cggtgcgcgc
      661 gtggttgatc gggttcagca gatggaagag ctcgactgat tcctcgccgt tgccgaggcg
      721 ggcggtggcc atcgccatcc agatggcggc gtgggtgtac tggccgccgt tctcgcggat
      781 gccggggacg taccccttga tgtagcccgg gtcgaccggc gacttgtcga acggaggcgt
      841 gaggagcagc acgagctggt gggcgcggtt caccagctgc gcccgcacgg cgtccatggc
      901 cgactcggcc cggttgggcg gcgccgcccc cgacaggacc gcccacgact ggctgatgga
      961 atcgatgcgg cactcctcgt tctgcgacga gccgagcggc gtgccgtcgt cgaaccagcc
     1021 gcgccggtac cacttgccgt cccaggccag ctccatggca tcggccaggc ggcgggcttc
     1081 gcgccggtag cgggccgccg cctcatcatc gccgcgggcc tggcagatcg gcacgaattg
     1141 cgagagcacc gcgtggagga accagccgag ccagacgctc tccccctttc cctcgatgcc
     1201 gacgcggttc atgccgtcat tccagtcgcc ggagccgaag agcggcaggc catgcgcgcc
     1261 ggccgtgagc cccctgtcga tggcccgccg gcaatgctcg taaagcgtgc ccgactcacc
     1321 ggatgtggtc ggcaggcggt acgactcggc ctcgtggggc tcgaggggtg gcgcttcgag
     1381 gaacggcagg accgtgtcga ggaccgaggc atcgcccgtg gtgcggacgt actgcgccgc
     1441 cgcccatggc agccagagga gatcgtccga acagcgcgtc cgcatgccgc gcccgtcgtg
     1501 cggatgccac cagtgctgca cgtccccttc cacgaactgc cggccggcgg cgcgcacgag
     1561 gtgttcgcgc gccacctccg gcgcggcgtg gagcagcgcc atgacatcct ggagctggtc
     1621 gcggaaaccg aacgcacctc ccggctggta gaagccggag cgggcccaga cacggcaggc
     1681 caggttctgg tagacgagcc agcggttgat catgaggtcg aacgaatcgt ccggcgtcgt
     1741 cacgcgaagc gtgtcgagac ggttggccca ggccaggcgc gtagcctcgc gcacgcggtc
     1801 cgcgtggccg agcccggcgt gccggcgcac cagcgccagg gccgcctcac gatccgccgc
     1861 ctcgcccagc acgcagttga tccggcgcgt ctctcccggg gcgatctcga ggacgcggtg
     1921 gagcccggcg cccggatcca tccccgcacc ggtccggccg ctcaacgcgc ggcgatacag
     1981 cgcggcggga ttcgacggcg tgccgttccg gccgatgaat tcggcgcgat cgccggtgac
     2041 cgaatcggga atctcgccga gcgcggcgaa cgcaacgcgt cccgggaagt cacggttcca
     2101 cgggttgtga gccaggatgg ccccgagctc cgccaccgac tcggtgacga cgtggagatg
     2161 atcgtcgggc cggctcggcc ccatcaccca ctcgtgccag gcaaagagcg agaggcggcg
     2221 cggcgcatcg ccgcggttgg tgatcgaaac gacgctgtgt ttcaccgggt cttccggatc
     2281 gacgacgagt tcgagctcat gctcgaggcc gcgccccgca tgacggaacg aggatcgccc
     2341 cgcctcgtgg cgcacaatcc atcgcccgcg gcgcaaaggc ggcggctcga gcgcgggcat
     2401 gaccggccac gactcgccgg tttcctcgtc acgcagccag atggcctcgc cggtggggcg
     2461 cgagaccggg tcgttggtga agggcgtcag ccggttctgc cggctgttgc cgctccaggt
     2521 gaaggccgcg ccggtggtgg tgacaagcgt gccgaagtgc gcgttggcca ggacgttgat
     2581 ccagggcgcg ggcgtctcgc ggtcgtcgtc gagtaccacg acggccgccg ggcgctcgtc
     2641 gttggtgaaa cctccgagac cgtgcggcag cacgaagggc ggcggctcga gcgttggaag
     2701 gtccggcgcg atggccgggg ccgcgcgccc gcgcgtcggg acgaagcgtt cggggtactc
     2761 cggctcggcg ggcgacatcg acacctggtc ggccagctcg ccgaggtcgc ctttcagcac
     2821 cgcgcgcgcc gcggacatga ggagagtgtg gtcggcctcg gccatcccgt cgcctcgcag
     2881 gaggaagacg ccgccggggc ggttgcgcca gccggcccac gagccgctct cgaggagggt
     2941 ttcgagctgg tgatgcatct cgtcgcgata atccatggcg tgctcgttga ggatgaccac
     3001 gtcggccgac aggttcttga gatgccagta ctcctgcgcc ttcagcacct ggcgcacgag
     3061 cggcaggtcg ttctcctcga ccacgcgcac gaggaggatc ggcacatcgc cggagatgcc
     3121 gaagggccag aggtcgggct gacccttcac gttggcggca tgcacctcgg gcgcggcccg
     3181 caacgagcga tcggggccca gcacgacgga ggccagccgc tggaagagtt gcgcctcgtc
     3241 ccgcgagagt cccaggtggc gcaactcgat ctgcgtccgc gcgaacgcca gcgagaaggc
     3301 gcgcgtggcg gcggccgggt cgcggtactt gttggccagg agcgtggccg cctcggcgga
     3361 gtcggccatg ccggtgccga aggccaggcg ggcaaatccc cctggcggca ggtggagccg
     3421 cacgcgcacc gcggcgatcg ggtcgagcac ggcgcccacg gtgccggtga gcggcctgtc
     3481 gagcgcgatc ggttcggcgg tcg
//
LOCUS       DASVOD010000423         5209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_498217, whole genome shotgun sequence.
ACCESSION   DASVOD010000423 DASVOD010000000
VERSION     DASVOD010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498217"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VF720_09735"
     CDS             complement(<1..1032)
                     /locus_tag="VF720_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7879679.1"
                     /translation="MNPPAPDDTTIDALLSENRTFPPDPAFTKRAWVKDDSVYAEADK
                     DFEAFWARFAGELEWMTPWNTVLEWTPPFAKWFVGGKLNASVNCLDRHLAGPRRNKAA
                     LIWEGEPGDQRTLTYFDLHREVCKFANCLKHLGVGKGDRVAIYLPMIPEAVIAALACA
                     RIGAVHSVVFGGFSSESLRDRINDQGAKLLVTADGGYRRGGSVPLKSMADEALAATPT
                     IENVVVVKRGEFPMHFQEGRDHWYHRLMQDASASCPPEPMDAEDPLFILYTSGTTGKP
                     KGILHTTGGYLTGVYATTKWVFDLKEDDVFWCTADIGWVTGHTYIAYGPLACGATQIV
                     FEGVPTYPDA"
     gene            complement(1079..2761)
                     /locus_tag="VF720_09740"
     CDS             complement(1079..2761)
                     /locus_tag="VF720_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7879680.1"
                     /translation="MSDPSPLAARLPKSFDCFRNYDSRTFTADAVAGITVGLVALPLA
                     IAFAIASGVPPQAGIYCAIVAGFLISALGGSRTQIGGPTGAFVVVVSGIVAAHGLDGL
                     FMCTMMGGVILLLLGLTGLGTAVDYIPRPVVIGFTNGIAVIIASTQLKEFFGLATGPD
                     PGHFIERMVAVANAMGNIQWPTALLSAAALLVLIVMARRRTRVPGSIVVLVLGTAVAW
                     VANLPVATVGSRFGDLPSGFPALTIPTFRPELVRELLSPALTVAMLGAIESLLSAVVA
                     DRMTGDRHNPNVELVAQGLANIASPLVGGLPATGAIARTATNIRSGARTPMAGMIHAA
                     TLLVIVLVGAPLARHIPLGVLGAILFVVAWNMGEWREVPEILRLAKADIAVWAITFGL
                     TVFADLTVAVQAGMILAALFYIRKVAITTTVSRVTPDYIEKGVPHSLQMNPLPDGVAV
                     YRIHGPFLFGATEKLSIIEEEMASLPPVVVLRLRNMTAIDATGVHALERLAGRLKSSG
                     RHLLICGLRDQPARLMSRSQFHRHIDPADILPSLAGAADRARELLGRRAASC"
     gene            2897..3841
                     /locus_tag="VF720_09745"
     CDS             2897..3841
                     /locus_tag="VF720_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7879681.1"
                     /translation="MELNVERGGFSRPEWMEEAVGRLLDTPLRVHLWGETGSGKNHLA
                     YRFRDHVMAAGRPFVEINVANLSDELLEGELFGCRRGAFTGAVADRAGVLERAEGGIL
                     FLNEVGELSAAGQAKLLTLLDTGLYRRLGDPTERRFTARVISATNRNLTRCVRAGAFR
                     EDLYYRLAQVRILVMSLRDREGEAVRMARAVLDRLAIERGRPLHFDSRAITRIEAYHW
                     PGNIRQLKSVVETLAWLAPGNLITGHALEEQIRQAGVDTEALFDRSFLRERVDELERV
                     EIEKALGATFGNKTQAAARLGLSVVGLRLKLKRLGFEE"
     gene            4006..4269
                     /locus_tag="VF720_09750"
     CDS             4006..4269
                     /locus_tag="VF720_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014240033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7879682.1"
                     /translation="MSLLYTVIIGLIVGVIAKMLMPGRDPGNWVLTILLGVAGAFVGT
                     LVGRALNLYREGEAAGFVMSVIGAMLLLLLYRLVTRRTAVKPV"
     gene            complement(4382..4753)
                     /locus_tag="VF720_09755"
     CDS             complement(4382..4753)
                     /locus_tag="VF720_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879683.1"
                     /translation="MSRFDALNATLAVALLTTLMMGVGTLQPTAAHAKGTAAIAQGGG
                     ASGAAIGTNRTVAGGDLSPQWFGKFWKQFKKIFSALIDFIDRVVDTTVELLVDGNPDG
                     SVRPDRWSPTRPEFPPHFPLA"
     gene            4987..>5209
                     /locus_tag="VF720_09760"
     CDS             4987..>5209
                     /locus_tag="VF720_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX7879684.1"
                     /translation="MSVLYTVFIGLIVGALAKLLVPGKDPGNWIITILLGIAGAFIGT
                     VIGRALNLYQEGEAAGFIMSVIGAMILLVL"
BASE COUNT          862 a         1627 c         1845 g          875 t
ORIGIN      
        1 ggcatccggg taggtcggca cgccttcaaa gacgatctgc gtggcgccgc aggccagcgg
       61 cccgtatgca atataggtat ggcccgtcac ccagccgatg tcggccgtgc accagaagac
      121 atcgtcttcc ttgaggtcga atacccactt cgtggtggcg tacacgccgg tcaggtagcc
      181 gcccgtggtg tgcaggatcc ccttgggctt cccggtagtg cccgacgtat agaggatgaa
      241 cagcgggtcc tcggcgtcca tcggctcggg cgggcaggac gctgacgcgt cctgcatcag
      301 acggtgatac cagtgatcgc gcccctcctg gaaatgcatc ggaaactcgc cccgcttcac
      361 gacgaccaca ttctcgatgg tgggtgtggc ggcaagcgcc tcgtccgcca tgctcttgag
      421 cggcaccgaa ccaccacgcc gatacccgcc atcggccgtg accagcagct tggccccctg
      481 gtcattgata cgatcgcgaa gcgattccga tgaaaacccc ccgaacacca ccgagtgcac
      541 cgccccgatg cgcgcacacg ccagcgccgc aatcaccgcc tccggaatca tcggcaggta
      601 aatcgccacg cgatccccct tgcccacgcc aagatgcttc aggcaattgg cgaacttgca
      661 gacttcgcga tgaagatcga agtaggtgag cgtgcgctgg tcgcccggct cgccttccca
      721 gatgagtgcg gccttgttac gacgcggtcc ggcgagatga cgatcgaggc agttcacgct
      781 ggcgttgagc tttccgccga cgaaccattt cgcgaagggg ggcgtccact cgagcaccgt
      841 gttccagggc gtcatccact cgagctcgcc ggcgaaccgc gcccagaagg cttcgaagtc
      901 cttgtcggct tccgcgtaga ccgagtcgtc tttcacccac gcccttttcg tgaaggccgg
      961 atcgggcgga aatgtgcggt tttcggagag gagggcgtcg atggtcgtgt cgtcgggggc
     1021 gggcgggttc atgggtgatc tccgtcggac ggttcggctc ggtgacgagc ccgaaatctt
     1081 aacaggaggc ggcgcgccgc cccagaagct cacgggccct gtcggccgcc ccggccaggg
     1141 atggcaggat gtcggccggg tcgatgtgcc gatggaattg tgatcgcgac atcaaccggg
     1201 ccggctggtc ccgcaggccg cagatcagga gatgtcgccc gctcgacttc aaccggccgg
     1261 ccagccgctc gagcgcatgc actcccgtgg catcgatggc cgtcatgttc cggagccgca
     1321 gcacgaccac cggcgggagc gaggccatct cctcctcgat gatcgagagc ttctcggtgg
     1381 ccccgaagag aaacggacca tggatgcggt acaccgccac gccgtcgggg agcgggttca
     1441 tctggaggct gtggggcacg cctttctcga tgtagtcggg cgtgacgcgt gacacggtgg
     1501 tcgtgatcgc caccttccgg atgtagaaga gcgccgccag gatcatgccg gcctgcaccg
     1561 ccacggtgag gtcggcaaag accgtcaggc cgaaggtgat ggcccacacg gcgatgtcgg
     1621 ccttcgcgag ccgcaggatc tccggcactt cccgccactc ccccatgttc cacgccacca
     1681 cgaagaggat ggcgcccagg acgccgagcg ggatgtgacg cgcgagcggc gcgccgacga
     1741 gtacgatgac gaggagcgtt gcggcgtgga tcatcccggc catcggcgtt ctggcgccgc
     1801 ttcggatatt ggtggcggtc cgggcgatcg ctccggtggc gggcagcccg cctaccagcg
     1861 gcgaggcgat gttggccaac ccctgggcca ccagttccac gttcgggttg tgccgatcgc
     1921 cggtcatccg gtcggccacc accgccgaca gcagtgactc gatggccccg agcatggcca
     1981 cggtcagtgc gggggagagg agctcgcgga cgagttcggg gcggaacgtg gggatggtga
     2041 gcgccggaaa ccccgatggc aggtcaccga accggcttcc gacggtggcc accggcaggt
     2101 tggccaccca cgccacggcc gtgccgagca ccaggaccac gatagagccg ggcacgcgcg
     2161 tccggcggcg ggccatcacg atcaggacga ggagcgcggc ggccgagagg agggcggtcg
     2221 gccattggat gttgcccatc gcgttcgcga cggcaaccat tcgctcgatg aagtgcccgg
     2281 gatcgggccc cgtggccagg ccgaagaact ccttgagctg cgtggaggcg atgatgacgg
     2341 cgatgccgtt ggtgaagccg atgaccaccg ggcgcgggat gtagtcgacc gcggtgccga
     2401 ggccggtgag gccgaggagc agcaggatga cgccccccat catcgtgcac atgaagaggc
     2461 cgtccaggcc gtgcgccgcc acgattcccg acacgaccac gacgaaggcg ccggtcggtc
     2521 cgccgatctg ggtgcgcgat ccacccaggg ccgagatcaa aaagccggcc acgatggcgc
     2581 agtagatccc cgcctgcggc ggcacgcccg aggcgatggc gaaggcgatg gcgagcggca
     2641 gcgccacgag cccgacggtg attcccgcga cggcgtcggc ggtgaaggtg cgggaatcat
     2701 agttgcggaa acagtcgaat gacttcggca ggcgtgcggc gagggggctg gggtcggaca
     2761 tcgccggaat cttactcccg gattcctccg gcgcaacaat cggctcgcac cccgttgaca
     2821 gctcgaacat cacccgggcg acactcttct ctgaacgagc cgacatccgc gaacccgttc
     2881 ggatccggga cgagtcatgg aattgaacgt ggagcgcggc ggcttttcgc ggccggagtg
     2941 gatggaggag gcggtggggc ggttgctcga cacgccgctg cgggtgcacc tgtggggtga
     3001 aacggggagc ggcaagaacc acctggccta ccgcttccgg gaccatgtga tggcggcggg
     3061 gcggccgttc gtcgagatca acgtggccaa cctgtcggat gagcttctcg aaggagagct
     3121 ctttggctgt cgccgcgggg ccttcaccgg tgcggtggcc gaccgggcgg gtgtgctgga
     3181 acgcgccgag gggggcatcc tcttcctcaa cgaggtgggg gagttgagcg cggcggggca
     3241 ggccaagctc ctcaccctgc tcgacaccgg tctttaccgg cgcctgggcg acccgacgga
     3301 acggcgcttc accgcccggg tgatttcggc caccaaccgc aacctcaccc ggtgcgtgcg
     3361 ggccggggcc ttccgggaag acctgtacta ccgcctggcg caggtgcgca tcctggtcat
     3421 gtcgctccgc gatcgggagg gagaggcggt gcggatggcg cgggccgtgc tcgatcggct
     3481 ggccatcgag cggggccggc cgctgcactt cgacagccgg gcgatcacgc ggatcgaggc
     3541 ctatcactgg ccggggaaca tccgccagtt gaagagcgtg gtggaaacgc tggcctggct
     3601 ggcccccggc aacctgatca ccggccacgc cctggaggag cagatccggc aggccggcgt
     3661 cgacaccgag gcgctattcg accgcagctt cctgcgcgag cgtgtggatg agctggaacg
     3721 cgtggagatc gagaaggccc tgggagccac gttcggcaac aagacgcagg ccgcggcccg
     3781 gcttggcctg tccgtggttg ggctgcgtct caaattgaag cgcctgggtt tcgaggaatg
     3841 acgcccacga cttgatgaac ccaggctcca gtgccttttg gggctgctcc ccgtatcgac
     3901 gaacggcgag gggaccggat catggttccc gggcagggtt gatggaatcg tcgtgatagg
     3961 cacaggcggc cggcgtcagc tggtcttcag aaaaggggta actccatgtc gctgctctat
     4021 accgtcatca tcggcctgat cgttggcgtg atcgccaaga tgctcatgcc cgggcgcgat
     4081 ccgggcaact gggtgctcac catcctgctc ggagtggccg gggccttcgt gggcaccctg
     4141 gtgggtcggg ccctgaacct ctatcgcgaa ggggaggcgg ccggattcgt gatgtcggtg
     4201 atcggggcca tgcttctcct gctgctgtac cggctggtga cgcgtcggac ggcggtgaaa
     4261 cccgtctgaa ccacggaaca actcgcggct tcatcggcgc gtccccttcc cgggggcgcg
     4321 ctttcgtttg cgtttcgaag tgtgacgggc gccggccggc agacgaccgg cgcccgatca
     4381 ttcaggccag ggggaagtgc ggtgggaact cggggcgcgt tggtgaccag cgatccgggc
     4441 ggacggagcc gtccgggttg ccatccacga gcagttcgac cgtcgtgtcg acgacgcggt
     4501 cgatgaagtc gatgagggcg ctgaagatct tcttgaactg cttccagaac ttgccgaacc
     4561 actgcggtga caggtcgccg ccggctaccg ttcgattggt gccgatcgcg gcgccggagg
     4621 cgccccctcc ctgtgcgatg gccgccgtgc cctttgcgtg ggccgcggtg ggttggagtg
     4681 tgccgacgcc catcatgaga gtggtgagga gcgcgacggc gagcgtggcg ttgagcgcgt
     4741 cgaaccgtga catgctggat ctcccggggg tttgtctggg ggacggttcg cgcaattgca
     4801 gggttcgtgc catcgaacgc ctgttgctaa gtcatgatct ggcaacggcg gcatgacaga
     4861 ccggcgggtg ttgcggcttg cggcatgata agttggtttc tggctccggg gactttgggc
     4921 gcaagtgctg accatgtcgc agtgcggcgc cggaaagttc ctttccggcg ctattggagg
     4981 gaccagatgt cggttctcta cacggtgttc atcgggctga tcgtcggggc gctggccaag
     5041 ctgctcgtgc cggggaagga tccggggaac tggatcatca ccatcctgct ggggattgcc
     5101 ggcgccttca tcggcacggt gatcgggcgg gccctcaacc tctaccagga aggggaggcg
     5161 gccggcttca tcatgtcggt gatcggcgcc atgatcctgc tcgtgctct
//
LOCUS       DASVOD010000424         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_498224, whole genome shotgun sequence.
ACCESSION   DASVOD010000424 DASVOD010000000
VERSION     DASVOD010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498224"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1403)
                     /gene="priA"
                     /locus_tag="VF720_09765"
     CDS             complement(<1..1403)
                     /gene="priA"
                     /locus_tag="VF720_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEX7879685.1"
                     /translation="MTRSDLFAEVAIPVPVRQTFTYRIPAALDGRIVPGLPVNVPFGR
                     RSAEGFVVSVGAAPAHLDPADIRDIRLAGAPEDVLPAGVLELTRWLADYYLCSWGEAI
                     KAALPGGIEPLRATPRRRRKVAPDAGPADPQVDRRASDGESDAFQSANGDRSPLARPL
                     TADQQQALAAIAPFLAEPAHRVFLLFGVTGSGKTEVYLEAAARVLARGGQVLAMVPEI
                     ALAPQMLAAFKSRFGDAVVLWHSALPTGQRRQAWRRVASGAATVVVGARSAVFAPFRN
                     LGLIVVDEEHEPAYKQDEAPRYHGRDVAVVRAHRLGIPIVLGSATPSLESFQNAGSGK
                     YFRLDMPRRIDDRPVPVVRVVPLLRKGDPRPEDDLPEGAREESAPDDRRGTGILSAPL
                     VRALRDRMKRAEQAILFLNRRGHSTVVRCDDCGWVARCPHCDIALTWHADRKELRCHY
                     CSHRSRDIDTCPDCAGR"
BASE COUNT          236 a          526 c          607 g          219 t
ORIGIN      
        1 catcggccgg cgcaatcggg gcaggtgtcg atgtcgcggc tccggtggga gcagtaatgg
       61 cagcgaagct ccttccggtc ggcgtgccag gtgagggcga tgtcgcagtg gggacatcgg
      121 gccacccagc cgcagtcgtc gcagcgcacc acggtggagt ggccgcgccg gttcaaaaag
      181 aggatggcct gctcggcccg cttcatgcgg tcgcgcagcg cgcgcaccag gggtgccgag
      241 aggatgccgg tgccgcgccg gtcgtcgggc gccgattcct cgcgggcgcc ttccgggaga
      301 tcgtcctcgg ggcgaggatc cccctttcga aggagcggca cgacgcggac taccggcacc
      361 gggcgatcat cgatgcgccg cggcatgtcg aggcggaagt acttccccga cccggcgttc
      421 tgaaacgact cgagcgacgg cgtggccgag ccgagcacga tcgggattcc gaggcgatgg
      481 gcccgtacga ctgccacgtc gcggccgtgg tagcggggcg cctcatcctg cttgtaggcc
      541 ggctcgtgct cttcatccac cacgatgagg ccgaggttgc ggaacggcgc gaagaccgcc
      601 gagcgcgcgc cgacgacgac ggtggccgct ccactggcca cgcggcgcca ggcctggcga
      661 cgttggcccg tcgggagcgc gctgtgccac aacaccacgg catctccgaa gcgactcttg
      721 aacgcggcca gcatctgcgg cgcgagcgcg atctcgggaa ccatggccag cacctgcccg
      781 ccgcgagcca gcacgcgcgc ggcagcctcg aggtatacct cggtcttgcc gctcccggtg
      841 acgccgaaga ggaggaagac gcgatgcgcc ggctcggcca gaaagggcgc gatggccgcg
      901 agcgcctgtt gctggtcggc ggtgaggggt cgggcgaggg gcgaacgatc gccgttcgcc
      961 gactggaacg cgtccgactc gccatcggac gccctccgat cgacttgcgg gtcggccgga
     1021 ccggcgtcgg gtgcaacctt tcgccggcgc cgtggcgtgg cccgcagcgg ttcgatcccg
     1081 cccggaagcg ccgccttgat ggcctcgccc caggagcaga ggtagtagtc ggcgagccag
     1141 cgggtgagtt cgaggacgcc ggccggcagc acgtcttcgg gagcccccgc gagacggatg
     1201 tcgcggatgt cagccggatc gaggtgcgcg ggcgcggcac cgaccgacac aacgaacccc
     1261 tcggcgctcc ggcggccgaa cggcacgttc accggaagtc cgggaacgat gcgcccgtcg
     1321 agcgcggcgg gaatgcgata ggtaaacgtc tggcgcaccg gcacggggat ggccacttcg
     1381 gcgaagagat cagatcgtgt cacgggaact cgactttccg gacggggcgt ccggtcgcgg
     1441 cggggcgatc agcgcttcgg tttcttggac gggtgcgtcg gatgcatgga ctgtagcaga
     1501 gccagccgct tctcctgctt ccgggcgccg cgggccttct tggcctcgag acgccgggcg
     1561 gggtcgggcg gggcggggcg cgcggcgg
//
LOCUS       DASVOD010000425         1974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_705472, whole genome shotgun sequence.
ACCESSION   DASVOD010000425 DASVOD010000000
VERSION     DASVOD010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705472"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VF720_09770"
     CDS             complement(<1..614)
                     /locus_tag="VF720_09770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEX7879686.1"
                     /translation="MPPRRSDSGGRSGGNRRSGTSGRSSGGRRPGAVGARPAPRPAPR
                     VPVRAIHPPVDPAELEADFRTFHAELARRGQELAEAPLNLVRRYGEMVVTRSALLNLV
                     SPGDRQRIFTRHIAECLAPELVATAQRAASLVDIGSGAGLPGIPLAIVAPGLRVLLVE
                     PRVRRAQFLEATALALGLGDRVEVFQGSAERLLNISQGELGAGLA"
     gene            complement(595..>1974)
                     /gene="mnmG"
                     /locus_tag="VF720_09775"
     CDS             complement(595..>1974)
                     /gene="mnmG"
                     /locus_tag="VF720_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX7879687.1"
                     /translation="TYEGGRSGEVSAPKMSDSLKELGFRMGRMKTGTPPRLLRSSIDF
                     RRLQPQPGDEPPSPFSHFTERLTVSQVECHLTTTTDETHAVIRRHLDKSPLYSGQITG
                     TGPRYCPSIEDKVVKFPDRSAHQVFLEPEGRATQEIYVNGISTSLPEECQEEFLHTIP
                     GLEEAVMLRPGYAVEYDCVQPDQLLPTLETRNVSGLFLAGQINGTSGYEEAAGQGLVA
                     GINAGLKLQEEPPFVLRRWEAYVGVMVDDLVNNCGDEPYRMFTSQAEYRLLLRQDNAD
                     ARLMDHGVRFGLVAPRDRARWSELRASIDRERGVLARRLSPENVSLLDLLRRPGLSYK
                     DLAPLRAETGPNESQPLDPGGLAAELLEIDVKYEGYVKRQMRQVEQLQALESKPIPPG
                     LLDGPLAGLSREAAEKLRRVRPVSVGQAGRLPGVSPADLTVLLILVQKWSADSASRSE
                     GAEHAPASI"
BASE COUNT          341 a          641 c          682 g          310 t
ORIGIN      
        1 gccagcccgg caccaagttc tccttgggag atgttgagga gccgctcggc ggacccctgg
       61 aacacttcca cccggtcgcc gaggccgagg gccagggcgg tggcttcgag aaactgggcg
      121 cgacggacgc ggggctcgac cagaagcacc cggagcccgg gagccacgat ggccagcggg
      181 ataccgggaa gcccggctcc cgagccgata tccaccagcg acgccgctcg ttgggcggtt
      241 gctaccagct ccggtgccag gcactccgcg atgtgccggg tgaagattcg ctgacggtcc
      301 cccgggctga caaggttcag gagcgcggag cgcgtgacca ccatctcgcc gtatcggcgg
      361 accaggttca ggggggcttc ggcgagctcc tgcccccgac gtgccagctc ggcatgaaag
      421 gtccggaagt cggcctcgag ctccgcgggg tcgacggggg gatggatggc ccgaacgggg
      481 acccgcggag ccggtcgtgg agccggacgg gctccgacgg caccggggcg gcgccccccg
      541 gaagaacgac cactggtccc ggaacgtcgg ttgccgccgg agcggccgcc ggagtcagat
      601 cgacgcgggg gcatgctccg ctccttcgga tcgagaggcc gaatcggccg accacttctg
      661 gacgagaatc aggaggacgg tgaggtcggc cggcgacacg cctggaagcc gccccgcctg
      721 tcccacggag acggggcgaa cgcgccgcag cttttcggcg gcctcccggc tcagtccggc
      781 gagcgggccg tcgagaagac cggggggaat cggcttcgat tcgagcgcct ggagctgctc
      841 cacctgccgc atctgccttt tcacataacc ctcatatttc acgtcgattt ccaacaactc
      901 ggcggccaaa ccgcccggat cgagaggttg gctctcattt gggcccgttt cggcccgcaa
      961 cggcgccaag tccttgtaag acaaaccggg acggcggagc aggtcaagca ggctcacgtt
     1021 ctccggggag agccggcgag ccagcacgcc gcgctcccga tcgatcgacg cgcgaagctc
     1081 ggaccagcgg gcacggtccc gcggcgccac caggccgaat cgaacgccat gatccatcag
     1141 gcgggcgtcc gcgttgtcct gacgcagcag caggcgatac tcggcctgcg aggtgaacat
     1201 gcggtaaggt tcatcaccac aattatttac aaggtcatcg accatcactc ccacgtaggc
     1261 ttcccaccgg cgaaggacga acggaggctc ctcttgaagc ttgaggccgg cattgatccc
     1321 ggccaccagc ccttgtcccg ccgcctcttc atatccggaa gtcccgttga tctggccggc
     1381 caggaagagt ccggacacgt tgcgcgtctc gagcgtggga agaagctggt cgggctggac
     1441 gcagtcgtac tcgaccgcgt aaccgggcct cagcatgacc gcctcctcga ggccgggaat
     1501 cgtgtgcagg aattcctcct ggcactcttc cggcaggctg gtcgaaatac cgttcacgta
     1561 gatctcctgc gtggcgcgcc cttcgggctc gagaaagacc tggtgggcgg agcgatcggg
     1621 aaacttcacc accttgtctt cgatcgacgg acagtagcgc ggtccggtcc cggtgatctg
     1681 gcctgagtag agcggcgact tgtcgagatg gcgacggatg accgcatgcg tctcgtcggt
     1741 ggtggtggtg aggtggcact ccacctgcga gacggtgagg cgttcggtga agtgggagaa
     1801 aggagacggc ggttcgtccc ccggctgggg ctggagccgc ctgaagtcga tgctgctccg
     1861 gaggagacgt ggcggcgtgc cggtcttcat ccgccccatg cgaaagccga gttccttgag
     1921 cgagtcggac atcttcgggg ccgacacctc gcccgagcgg ccaccttcgt aggt
//
LOCUS       DASVOD010000426         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_289811, whole genome shotgun sequence.
ACCESSION   DASVOD010000426 DASVOD010000000
VERSION     DASVOD010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289811"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF720_09780"
     CDS             complement(<1..266)
                     /locus_tag="VF720_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879688.1"
                     /translation="MRSIAARTGVSSLVILVTLSGPPAAGVTLAAEPVGAPSNMRHGP
                     LTAAVARRDSLTAPDIDALVTRALAGSPDLAALEARAEGARRAA"
     gene            complement(346..693)
                     /locus_tag="VF720_09785"
     CDS             complement(346..693)
                     /locus_tag="VF720_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879689.1"
                     /translation="MIRRLLSLALIFAVAAGTFVPVGLTRCEVKKSCCGCGPAMEMTG
                     CCCGPEAAGTSAPVPAPRVDIPLDWTALAVDAVPAPATNQEILSRALAHSVLDPSRAD
                     HVPLFLSHHAFLI"
     gene            926..>2291
                     /gene="ppk1"
                     /locus_tag="VF720_09790"
     CDS             926..>2291
                     /gene="ppk1"
                     /locus_tag="VF720_09790"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX7879690.1"
                     /translation="MPRRPAPAKVTRGRRPAGLPLADVDDLRALLINRELSWIEFNRR
                     VLGEALDTSHPLLERLKFLSIFATNLDEFFMIRVSGLIQQVDAGVARLSPDGMSPQAQ
                     LKSISDCLRPLLEEQMACYRQRLLPELASNGIHLVPWDSLTPNESAEMGRQFEERIFP
                     ILTPLAVDPSHPFPYISNLSLNLAVELSVWNEKEEVEETRFARVKVPPVVPRLVKLPG
                     DGDRYVLLEEVIAAHIGRLFPEVKVTVCHPFRVTRDADFEIEDDEARDLLKEIEEQLR
                     RRRFGSAVRLEVDAAMPESMRRYLTTALELEEIDVYQVDGPLALHDLMSLYRLDRPHL
                     KDAPFRPGVPAVLEGEESLFDVLARQDVLLHHPYDSFGPVVDFIRTAAEDPRVVAIKQ
                     TLYRTSGDSPVVAALIRASERGKQVAVLVELKARFDEESNIGWAKKLEAEGVHVVYGL
                     LGLKT"
BASE COUNT          384 a          777 c          782 g          348 t
ORIGIN      
        1 tcggccgccc gtcgggcccc ttcggcccgg gcctcgagcg cggccaggtc gggtgagccg
       61 gccagtgcgc gcgttaccag cgcgtcgatg tcgggagccg tgaggctgtc gcgccgggcc
      121 acggccgcgg tcagcggccc atgccgcatg ttcgatggtg cgccgaccgg ttcggcggca
      181 agcgtcacgc cagcggccgg cggaccggac agggtaacca ggatgacaag cgatgaaacg
      241 cccgttcggg cggcaatgga acgcacggct cctcctcgaa gaaaacggca cgacggcgcc
      301 ccggacgcgg gacgcggaca cacgtatgcc tgtggaggag ggcgactaga tcaggaaggc
      361 gtggtgcgag aggaagagcg ggacgtggtc ggctcgtgag ggatcaagga ccgagtgggc
      421 gagggcccgg gaaagaattt cctggttcgt ggccggggcc ggcacggcgt cgacagccag
      481 ggccgtccag tcgagcggga tgtccacccg cggcgccggg accggggcgg acgtgccggc
      541 cgcttcgggc ccgcagcagc acccggtcat ctccatggcc gggccgcagc cgcagcatga
      601 cttcttcacc tcgcagcgag tcaggccgac gggcacgaag gtgcccgcgg cgaccgcgaa
      661 aatcagggcg agggacagga ggcgtcttat catcagggtc aggatgccgt cggagggacg
      721 ggcgtgtcaa tgttatcggc cggatgttgg ttccccggag cctcccggaa gattggactt
      781 gcccgcgccc ggttggctag gatagaagga gatcgtaatc gcttaagtcg ttacgtgaca
      841 ggaaggtgac aaccgtcacg tgccctggcc ggccgccgcg ccaatccgcc cggaaccggg
      901 gactcccggt cgagtcatga ggaccatgcc ccgtagaccc gctcccgcaa aggtgacccg
      961 aggccggcgc ccggccggcc tccccctcgc cgacgtcgac gacctccggg cactcctcat
     1021 caaccgcgaa ctctcgtgga tcgaattcaa tcgtcgcgtc ctcggtgaag cgctcgacac
     1081 gtcgcaccca ctcctcgagc gcctgaagtt cctctccatc ttcgccacca acctcgacga
     1141 gttcttcatg atccgcgtgt ccggactcat ccagcaggtg gatgccggcg tggccaggct
     1201 ctcgcccgac ggcatgtcgc cgcaagccca gctcaagtcg atctccgatt gcctgcgccc
     1261 gctcctcgag gagcagatgg cctgctaccg ccagcgcctc ctccccgagc tggcctcgaa
     1321 cggcatccac ctcgtgccgt gggactcctt gacgccgaac gaatcggccg agatggggcg
     1381 ccagttcgag gagcggatct tcccgatcct gacgccgctg gccgtcgacc cctcacaccc
     1441 cttcccgtac atctcgaacc tgtcgctcaa cctggccgtc gagctgtcgg tctggaacga
     1501 aaaggaggag gtggaggaaa cgcgcttcgc ccgcgtgaag gtgccgccgg tcgtgccgcg
     1561 cctcgtcaag cttccgggcg acggcgatcg ctatgtgctg ctcgaggaag tcatcgccgc
     1621 ccacatcggg cgcctcttcc cggaagtgaa ggtgacggtg tgccacccgt tccgcgtgac
     1681 gcgcgacgcc gacttcgaga tcgaggacga cgaggcgcgc gacctcctca aggaaatcga
     1741 ggagcagctc cgccgccggc gcttcggatc ggccgtgcgc ctcgaggtgg atgccgccat
     1801 gcccgagtcg atgcggcgtt acctgaccac cgcgctcgag ctcgaggaga tcgacgtgta
     1861 ccaggtggat ggccccctgg cgctccacga cctcatgagc ctctatcgtc tcgaccggcc
     1921 ccatttgaag gacgccccgt tccggccggg cgtgcccgcc gtgctggaag gggaggagag
     1981 cctgttcgac gtgctggccc gccaggacgt cctcctccac catccgtacg actcgttcgg
     2041 accggtggtc gatttcatcc ggaccgccgc cgaagatccg cgcgtggtgg ccatcaagca
     2101 gacgctctac cgcaccagcg gtgattcacc ggtggtggcg gccctcatcc gggccagcga
     2161 gcgcggcaag caggtggcgg tgctggtgga gctcaaggcg cgcttcgacg aagagagcaa
     2221 catcggctgg gccaaaaagc tggaggccga aggcgtgcac gtggtctatg gcctgctcgg
     2281 gctgaagacg c
//
LOCUS       DASVOD010000427         2486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_549485, whole genome shotgun sequence.
ACCESSION   DASVOD010000427 DASVOD010000000
VERSION     DASVOD010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549485"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF720_09795"
     CDS             complement(<1..325)
                     /locus_tag="VF720_09795"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX7879691.1"
                     /translation="MFARLGRLFRAWMGFFISKAEDPEIMLQDAIEEMRTTMPKLNAV
                     LVATRATVLRLEEERDALLRQDKNLTGSIQAALRDGSAAARSVAEEDAIQLQQLRIDL
                     AATEDQ"
     gene            complement(315..1016)
                     /locus_tag="VF720_09800"
     CDS             complement(315..1016)
                     /locus_tag="VF720_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879692.1"
                     /translation="MAKKWYDFFVVTDRPGDPAGGGKPGPEPAEAAGTAAGPSPTSAA
                     PKRAAELAPQSADPAFTAPVADPIAFDEIYAAAQIAPPAHGYSILKVAEMLGSEHLRD
                     LASDLKKRSILVALDAAGVAVDDVVQDAVRRDRALDTYERLLQKSLEELRAGSEAENR
                     RIEDEINSRVAELKTRLEENRRKLREEESGLEAWRSQKVREEARIAEAVGYFVSENPV
                     TTSDKTTPSGGDHVR"
     gene            1585..>2486
                     /locus_tag="VF720_09805"
     CDS             1585..>2486
                     /locus_tag="VF720_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879693.1"
                     /translation="MKLGFQSRLGFLFAALLAVTLIVVPYGRAEDGDEEYEPSQPPLE
                     VPMPGAKATPMSVTAAQTAVIYFSELEQVSSSVTEPYPSIRTWMPNELEPPAEPHFPP
                     APELGAGSEPPELFVASPGPTNNYMGLDDIAMVDSLYIIIPPDVSGGVGLTKVMEAFN
                     NNYRIRDKATGVTISTVGTATFWAPVVAVAERLSLTDPRVLYDPYNNRWITVMQTVTT
                     GAGKLLLGVSQTSDPSGAWNLYSFNTGLRLDFPIVGFNKNWVSISINAYTAAGSFTNG
                     INLVVDYPQARAGVGTGTLFTHAAG"
BASE COUNT          390 a          864 c          772 g          460 t
ORIGIN      
        1 gctggtcctc ggtggcggcc aggtcgatgc ggagttgctg gagctggatg gcatcctcct
       61 cggccacgct gcgggccgcg gccgagccgt cgcgcagggc ggcctggatc gagccggtga
      121 ggttcttgtc ctggcggagc agcgcgtcgc gctcttcctc gaggcgcagt accgtggcgc
      181 gcgtggccac cagcaccgcg ttcagcttcg gcatggtggt gcgcatctcc tcgatggcgt
      241 cctggagcat gatctccggg tcttccgcct tggagatgaa gaagcccatc caggcgcgga
      301 acagccggcc caatctagcg aacatggtca cctccgctcg gggtggtctt gtcggacgtg
      361 gtcacgggat tctcgctgac aaaatagccc accgcttcgg cgatgcgggc ttcttcgcgc
      421 actttctgcg accgccaggc ctcgaggccc gattcttcct cgcgcagctt ccggcgattt
      481 tcctcgagcc gggtcttcaa ttcggccacc cggctgttga tttcgtcctc tatccgcctg
      541 ttctcggcct cgctgccggc cctgagctct tccagactct tctggagcag gcgttcgtag
      601 gtgtcgagcg cccggtcgcg ccggacggca tcctgcacca cgtcgtccac cgccacgccg
      661 gccgcatcga gcgccaccag gatcgagcgc ttcttgaggt cggaggccag gtcgcgcagg
      721 tgctcgctcc ccagcatctc ggccactttc aggatggagt agccgtgggc cggtggggcg
      781 atctgcgcgg ccgcatagat ctcgtcgaag gcgatggggt cggcgaccgg tgcggtaaag
      841 gcagggtcgg cgctttgcgg tgccaactcc gccgcgcgtt tgggagccgc ggatgtcggc
      901 gagggccccg cggcggttcc ggccgcttcc gccggctccg gccccggctt tccccccccg
      961 gccggatccc ccggccgatc ggtgaccacg aaaaagtcgt accacttctt cgccacgttc
     1021 aggagcctcc acgataggtt cgcgcggcat tctaacagct tgcgacggat cgggttggta
     1081 cggacgggcc ggcagccggt cgcacggaat cggcggccca ggtgggtggc cgcccgcccg
     1141 ggcccccgag gttcgatccg acgcgtgagc ctcgtcgatg aatttcgttc agtaattcga
     1201 cagctcgccg cgaacggcca ccttggttgc ggctgcggtt ccgcctgacg gatgtggcgc
     1261 gaacgcccgt atgtggcatt tcgccacaga gccttgctcg ggaggtttgc gcggacctct
     1321 tgatcctgag tcaaatgcag aattcaatca gaatttcttt gaccctcatc tcctataact
     1381 acataatcga atcagagctt cgccgccgct acggtcgaag cgaaacaggc cgctccttcg
     1441 gcacaacgcg atccaaccca atcccccagg cttgagcgtt gcttgctatt ccgccaagcc
     1501 tccggttgcc aggtcgatcc catccgttga cggcccgcag catcggcggt gcaccccgcc
     1561 ggctcctcaa cctcggagga atggatgaag ctcggattcc agagtcgctt gggtttcctc
     1621 ttcgccgcgt tgctcgcggt caccctcatc gtcgtgccgt atggccgcgc ggaagatggt
     1681 gacgaggaat acgaacccag ccagcccccc ctcgaagtac cgatgcccgg cgccaaggcc
     1741 acgccgatgt cggttaccgc cgcgcagacg gccgtgatct acttcagcga gctggagcag
     1801 gtctcctcgt cggtcaccga accgtacccg agcatccgga cctggatgcc caacgagctc
     1861 gagccaccgg ccgaacctca cttcccgccg gcgccggaac ttggcgcggg gtcggagcca
     1921 cccgaactct tcgtggcctc gccgggaccc accaacaatt acatgggcct cgacgacatc
     1981 gcgatggtcg actcgctgta catcatcatc ccgccggacg tgtccggcgg cgtgggcctc
     2041 accaaggtga tggaggcgtt caacaacaac taccgcatcc gcgacaaggc gaccggtgtc
     2101 acgatctcca ccgtgggcac cgccacgttc tgggcccccg tcgttgcggt ggcggaacgg
     2161 ctttccctga ccgatcctcg ggttctctac gatccctata acaaccgctg gatcaccgtc
     2221 atgcagacgg tgaccacggg ggcgggcaag ctgctcctcg gcgtgtcgca gaccagcgac
     2281 ccgagcggcg cctggaacct ctacagcttc aacaccggcc tgaggctcga cttcccgatc
     2341 gtcggcttca acaagaactg ggtctcgatc tcgatcaacg cgtacaccgc ggccgggtcg
     2401 ttcaccaacg gcatcaacct ggtggtggac tacccgcagg cgcgcgccgg cgtgggcacc
     2461 ggcaccctct tcacgcatgc cgccgg
//
LOCUS       DASVOD010000428         4807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_915304, whole genome shotgun sequence.
ACCESSION   DASVOD010000428 DASVOD010000000
VERSION     DASVOD010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915304"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2391)
                     /gene="topA"
                     /locus_tag="VF720_09810"
     CDS             complement(<1..2391)
                     /gene="topA"
                     /locus_tag="VF720_09810"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX7879694.1"
                     /translation="MKKTAKKSAVKAAPKTARRKKGAPDAESAVALEELEDAYETDGD
                     EPDAEGVAERPAGKRSPRHLVIVESPSKAKTLTKFLGRGFTVMASNGHIMDLPTSKLG
                     VDIENDFQPEYVAIRGKKKILDDLKKKAREVDVVYMAPDPDREGEAIAWHLRNWLGKA
                     NGTLRRLTFNEITERAVKDSLNHAREIDENLVNAQQARRVLDRLVGYKVSPLLWNILR
                     YGLSAGRVQSVALRIICDREKEIRAFNAVEYWTIEARLAAPGSPPSPPIPEPEAQAQY
                     VVGTEEKAPVAPLGPGEFYARLIKVGDEKPELTDGTTATSLVESLQGRPFTVTEVKKR
                     ERRKNPAPPFITSTLQQDAFKKHRYSSQKTMVLAQQLYEGIDLGEGPVGLITYMRTDS
                     TRIAQEALDAVRGHIRSSYGDRYLPETARTFPTKKAAQDAHEAVRPSEVDRTPESLAR
                     YLQPDLLKLYTLIWNRFVASQMTPAVYETTSVDIRVDRAAGDPVTFRASGTVPIFDGF
                     TRLWASLDEEEEEPTLPALEEGTRVEDRGVVPSQHFTEPPPRFTEATLVKALEENGIG
                     RPSTYATIVSTILTRDYVLRDRGKLTPTELGMTVTDLLIKTFPDIFNVDFTAQMENEL
                     DRIETGEEEWHSVIKGFWDPFASDLTKAEGQKAELKAGLQEETDILCPKCGEETGSKM
                     IKKYGRNGIFLACPRYPDCKATQPYDQAEVETTDEVCEKCSGPMVVKRGRFGRFLACS
                     NYPDCKSTRPYRIGVPCPEKDCGGQLSEKRSKRGKMFYGCDRWPECNFATWDRPVPV"
     gene            complement(2388..>4807)
                     /locus_tag="VF720_09815"
     CDS             complement(2388..>4807)
                     /locus_tag="VF720_09815"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX7879695.1"
                     /translation="IDEARTPLIISGPVAHSIQYYDQLKPSVERVVRKQTAVTNTLLA
                     EAEKLVADGAKKGGDEEYELGVKLLQVRRGAPKNRRFMKLIAEEPGLNRIIQRTELDF
                     IRDKRMNEIDAVLCYSIDEKSHAIDVTDLGRETIAPQDPEMFVLPDIALKMAEIDGAE
                     MEPAEKVKAREQAHRKYAERADRIHSALQMLKAYSLYEKDVEYVIQDGKIVIVDEFTG
                     RQMPGRRYSDGLHQAIEAKEGVKVEGETQTLATITLQNFFRMYKKLAGMTGTAETEAG
                     EFHGIYKLDVVVIPTNRQARRMDLDDVIFRTRREKYNAIIDEIAKMHEAGTPVLVGTV
                     SVDVSETLSRLLARRGIRHSVLNAKQHQREAEIVSMAGLTGAVTIATNMAGRGTDIKL
                     GPGVLYDDLEKNFPPPGRKGPTEQVDIRGLAIIGTERHESRRIDRQLRGRSGRQGDPG
                     SSRFFLSLEDDLMRLFGAERISGIMEKMGVQEGEVIEHPWVTKAIERAQKRVEEHNFS
                     IRKHLIEYDDVMNKQREVIYEQRLRVLEGESLRESMLEDLDAYVASVVENHLDESIPV
                     DDWDLRQPAAELEMSLLNPFPTAAIEAGSLDKAGIVEAFQELARAAYTRKEEELSEPI
                     MREVERRVAMSVIDENWRDHLHEMDLIKEGISLRSFGQKDPLLEYKKEAFSTFAEMND
                     VIRTETVKRLFRVSLVREAPEPPRRPAPAMTMTHSGASAFGPPQAAGMAGPARMAPPP
                     GGLPGGLPGNMAPQGGLPGNTARDSAPAPARTQAVDPAIAADKIGRNDPCPCGSGKKY
                     KKCHGVQ"
BASE COUNT          660 a         1563 c         1613 g          971 t
ORIGIN      
        1 caccggcacc ggccggtccc acgtggcgaa gttgcactcc ggccagcgat cgcagccgta
       61 gaacatcttg ccgcgcttgc ttcgcttctc ggaaagctgg ccgccgcagt ccttttcggg
      121 gcacggcacg ccgatccggt agggacgcgt gctcttgcag tccgggtagt tcgagcaggc
      181 aaggaagcgc ccgaaccggc cgcgcttgac gaccatcggc cccgaacact tctcgcacac
      241 ctcgtcggtc gtctcgacct cggcctggtc gtagggctgg gtggccttgc agtcgggata
      301 gcgcgggcag gccaggaaga tgccgttgcg gccgtacttc ttgatcatct tcgagccggt
      361 ttcctcgccg cacttcgggc agaggatgtc ggtctcctcc tggagacccg ctttcagctc
      421 ggccttctgc ccctccgcct tcgtcaggtc ggacgcaaac ggatcccaga accccttgat
      481 gaccgaatgc cactcctcct cccccgtctc gatccggtcg agctcgttct ccatctgcgc
      541 cgtgaagtcg acgttgaaga tgtccgggaa ggtcttgata aggaggtcgg tcacggtcat
      601 cccgagctcg gtcggggtga gcttgccgcg atcgcgcagg acgtaatcgc gcgtcaggat
      661 ggtggagacg atggtggcgt aagtggacgg ccggccgatg ccgttttcct ccagcgcctt
      721 gaccagcgtg gcctcggtga agcggggcgg cggctccgtg aagtgctggc tcggcacgac
      781 gccgcggtct tccacgcgcg tcccctcttc gagcgccggg agcgtcggtt cctcttcctc
      841 ctcgtcgagc gaggcccaga gccgcgtgaa gccatcgaaa atcgggacgg tacccgaggc
      901 gcggaaggtg accggatccc cggccgcgcg gtctacccgg atgtcgaccg atgtcgtttc
      961 gtagacggcc ggtgtcatct ggctggccac gaaacggttc cagatcagcg tgtagagctt
     1021 caggagatcg ggctgcaggt agcgcgccag gctctcgggc gtgcggtcca cctcgctggg
     1081 acgcacggct tcgtgcgcat cctgcgcggc cttcttggtc gggaaggtgc gggccgtttc
     1141 cggaaggtag cggtcaccgt agctcgatcg gatatggccg cgcacggcgt ccagggcttc
     1201 ctgggcgatg cgcgtcgaat cggtacgcat gtaggtgata aggccgaccg gtccttcacc
     1261 gaggtcgatt ccttcgtaca gctgctgcgc caacaccatg gtcttctggc tcgagtagcg
     1321 gtgcttcttg aacgcgtcct gctggagcgt ggaagtgatg aacgggggcg ccggattctt
     1381 gcggcgctcg cgcttcttga cctcggtgac ggtaaacggg cggccctgca gcgactccac
     1441 cagcgaggtg gcggttgtgc cgtcggtgag ctcgggcttc tcgtcaccca ccttgatgag
     1501 gcgggcgtag aactccccgg gaccgagcgg cgccaccggc gccttctcct cggtacccac
     1561 cacgtactgg gcctgggcct ccggctccgg gatcggcggc gacggcggcg atccgggcgc
     1621 ggcgagacgg gcctcgatcg tccagtactc cacggcgttg aacgcccgga tttccttctc
     1681 gcggtcgcag atgatgcgga gcgccaccga ctgcacgcgt ccggccgaca ggccgtagcg
     1741 gaggatgttc cagaggaggg ggctcacctt gtacccgacc agccggtcga gcacgcggcg
     1801 ggcctgctgg gcgttgacca ggttctcgtc gatctcgcgg gcgtggttca gcgaatcctt
     1861 gaccgccctt tccgtgatct cgttgaacgt gaggcgtcgc agcgtcccgt tggcctttcc
     1921 cagccagttc cggagatgcc aggcgatggc ctccccttcg cggtcggggt cgggcgccat
     1981 gtagaccacg tcgacctcac gcgccttctt cttcaggtcg tcgaggatct tcttcttgcc
     2041 gcggatggcc acgtactcgg gctggaagtc gttctcgatg tcgacgccga gtttggaggt
     2101 gggaaggtcc atgatgtggc cgttgctggc catcacggtg aaaccgcggc cgaggaactt
     2161 ggtgagcgtc ttggctttcg acggggactc gacgatcacg agatgacggg gcgagcgctt
     2221 tccggccgga cgttcggcca cgccttcggc gtccggttcg tctccgtcgg tttcgtaggc
     2281 gtcctcgagt tcctcgaggg ccacggccga ctcggcgtcg ggcgcgccct tcttccggcg
     2341 cgcggtcttc ggcgcggcct tgacggctga ttttttcgcg gtctttttca ctggactccg
     2401 tggcacttct tgtacttttt cccgctgccg cacgggcagg ggtcgttgcg accgatcttg
     2461 tcggccgcga tggccgggtc gaccgcctgg gttctggcgg gtgcgggcgc actgtcacga
     2521 gcggtgttgc cgggcagccc cccctggggc gccatgttgc ccgggagccc accgggaagt
     2581 ccgccggggg gcggtgccat ccgggccgga ccggccatgc cggccgcctg gggtggaccg
     2641 aatgcgctgg cgccggagtg ggtcatggtc atggccggcg cggggcgtcg cggcggttcc
     2701 ggcgcctcgc ggacgaggct cacccggaaa agccgcttca ctgtttcggt ccggatcacg
     2761 tcgttcatct cggcgaacgt gctgaacgcc tccttcttgt actcgagcag cggatccttc
     2821 tgcccaaacg accggaggct gattccctcc ttgatcaggt ccatctcgtg gagatgatcg
     2881 cgccagttct cgtcgatgac cgacatggcc acccgccgct ccacttcccg catgatcggc
     2941 tcggagagct cctcctcctt gcgggtgtag gcggctcggg ccagctcctg gaaggcctcg
     3001 acgatgccgg ccttgtccag cgagccagct tcgatggcgg cggtggggaa aggattcagg
     3061 aggctcattt cgagctcggc cgccggctgc cgcaagtccc agtcatcgac cgggatggat
     3121 tcatccaggt ggttctcgac gaccgaagcc acgtaggcat cgaggtcctc gagcatcgac
     3181 tccctgaggc tctccccttc gagtacgcgg aggcgctgtt cgtaaatcac ctcgcgctgc
     3241 ttgttcatca cgtcgtcgta ttcgatcagg tgcttgcgga tggagaagtt gtgctcctcg
     3301 acgcgtttct gggcgcgctc gatggccttg gtcacccagg gatgctcgat gacctcccct
     3361 tcctgcacgc ccatcttctc catgatgccg ctgatccgct cggcgccgaa caggcgcatc
     3421 aggtcgtcct cgagcgagag gaagaagcgg gatgagcccg gatccccctg gcggcccgag
     3481 cggccacgga gctggcggtc gatgcggcgg ctctcgtgcc gttccgtgcc gatgatggcc
     3541 agcccgcgga tgtcgacctg ctcggtcgga cccttgcgcc cggggggcgg aaagttcttt
     3601 tcgaggtcgt cgtacagcac gcccggcccc agcttgatgt cggtgccgcg gcccgccatg
     3661 ttggtggcga tggtgaccgc gcccgtgagc ccggccatcg acacgatctc ggcttcccgc
     3721 tggtgctgtt tggcgttgag gaccgagtgg cggatgccgc gccgggcgag gagtcgagac
     3781 agtgtctccg acacgtccac cgacacggtg cccaccagga ccggcgtgcc cgcctcgtgc
     3841 atcttggcga tctcgtcgat gatggcgttg tacttctcgc ggcgtgtccg gaagatgacg
     3901 tcgtccaggt ccatgcggcg ggcctggcga ttggtgggga tgacgaccac gtcgagcttg
     3961 tagatgccgt ggaactcccc cgcttccgtc tcggccgttc cggtcatgcc cgccagcttc
     4021 ttgtacatgc ggaagaagtt ctggagggtg atcgtggcga gggtctgggt ttccccctcg
     4081 accttcaccc cctccttggc ttcgatcgcc tggtgcagtc cgtccgaata gcgccgaccc
     4141 ggcatctggc ggccggtgaa ctcgtcgacg atgacgatct tcccgtcctg aatgacgtac
     4201 tcgacgtcct tctcatagag cgagtaggcc ttcagcatct ggagagccga gtggatccgg
     4261 tcggcccgtt cggcgtactt gcgatgcgcc tgctcccggg ccttcacctt ttcggcgggc
     4321 tccatctcgg cgccgtcgat ctcggccatc ttgagggcga tgtccggcag gacgaacatc
     4381 tcggggtcct gcggggcgat cgtctcgcgc cccaggtcgg tgacgtcgat cgcgtgggac
     4441 ttctcgtcga tggaatagca gagcaccgcg tcgatctcgt tcatcctctt gtcgcggatg
     4501 aagtcgagct cggtccgctg gatgatgcgg ttcagccccg gctcctcggc gatgagcttc
     4561 atgaaccggc ggttcttggg agcgccgcgg cgcacctgca gcagcttcac gccgagctcg
     4621 tactcctcgt ccccaccctt cttcgcccca tcggccacca gtttttcggc ctcggccagg
     4681 agggtgttgg tgacggcggt ctgcttgcgc accacgcgct cgacggacgg cttcagctgg
     4741 tcgtaatact ggatcgagtg tgcgaccggc cccgagatga tgagcggcgt ccgggcctcg
     4801 tcgatga
//
LOCUS       DASVOD010000429        14362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1022237, whole genome shotgun sequence.
ACCESSION   DASVOD010000429 DASVOD010000000
VERSION     DASVOD010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 14362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14362
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022237"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1809)
                     /locus_tag="VF720_09820"
     CDS             complement(<1..1809)
                     /locus_tag="VF720_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7879696.1"
                     /translation="MSPAIPIPPPQSLRERLGALRNLPPFLAMVWRTNPGLTLGSLAL
                     RLIRALLPVLTLYVGKLIIDEVIAQASTPAAGVSFGAWLQGGHADRLIGLVFLEFALA
                     VFADVLGRVVSLLDSLLAEQFSNVTSVRLMQHAATLDLEDFEDSELQDRLERARRQTS
                     SRMTLTSQLFGQAQDIVTILGFGAGLAIYAPWLIVLLLVALVPAFLGEAHFNARSYAL
                     THSRTPEQRELDYVRQTGASVETAKEVKIFGLHGFLIDRYRELADSFYQANRRLALKR
                     AGWGGLFTTLGTIGYYVAYAVIIWRTVAGHDTVGDLTFLAGSFRRLRTLLEEILIGFS
                     QTAGQALYLEDLFSFFEIRPEIRSPANPRPFPAPIRRGVAFENVGFSYPGSDRWAVRN
                     LDFTLQAGEVLALVGENGAGKTTLVKLLARLYDPDEGRILLDGHDLKEYSLEELRANI
                     GVIFQDFVRYHLTAGENIAVGRIEAREDRARIAEAAHHGLADEVIDRLPFGYEQIIGK
                     RFRKGVDLSGGEWQKVAIARAYMRRAQLLILDEPTAALDARSEFDVFQRFKELTAGRT
                     AVLISHRFSSVRMADRILVLANGTVESQGTHEELLAA"
     gene            complement(1983..3596)
                     /locus_tag="VF720_09825"
     CDS             complement(1983..3596)
                     /locus_tag="VF720_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879697.1"
                     /translation="MTGATRDTPLADKALRRLFLTLFVRGRTAVARGKASTPKSVGER
                     LFWVLLSYTAFGIVAFAGIGQPVLVLSAMLHGFSFFCLAIFLMTTAGETLFNREEPDI
                     LMHRPVEAGNVLRAKVRVMLEVAFWIAGALNLFGLLVGTFAPDSDWRFPFVHLVSTGL
                     SALFTVGIVVLSYELCLRWIGRERLESLLTTVQVGFSILIVLASQFGPRVIGKTGAHT
                     IREMPAWGLALPPVWFAGFDDALAGSGAIRSWAVAGLAVGAALLVLWLALGRLAGAYA
                     AGLQTAVASSPARSSRPPRRWLHALVEHPPLRWWVGGSVPRAAFALTLAYLFRDRETR
                     LRVYPGVVPMIVIPALTLLENMKSTGFGAFSLAFAGMYVGLIPMLALDTLRHSSQAPA
                     AMLFRAAPLPGPAPFCLGARRAVTLLFGFPVLLALAAAIALLDSDPMHLLLLLPGLLL
                     SPILTLFPCINGHAVPLSRPVDEGTQIARTIVLIALFAAAAMVAGVAALCWRREVFGG
                     FLAVEAVIVLLGRWIMGRAIAQARWAPAG"
     gene            complement(3593..4363)
                     /locus_tag="VF720_09830"
     CDS             complement(3593..4363)
                     /locus_tag="VF720_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7879698.1"
                     /translation="MIVLDHLTRTFGTQIAVDDLSFSVPAGQIVGFLGPNGAGKTTTL
                     RMLTGLLEPTSGTATIGEFDLLTQSIEVKRRIGFVPDSGAVFESLTGLEYLEMVAALY
                     DIPADQAEARIRQFLAFFDLGFDTLTDKQLGAYSKGMRRKVVITSALLHNPPVVFFDE
                     PLEGLDANAAVGFKALIQSLAREGKTILYSSHILDVVERVCDRVIIIDKGKLLVDGAP
                     DALIAAHGAETLERLFTQLTGNTDLERRAEDFARTFRT"
     gene            complement(4475..5692)
                     /locus_tag="VF720_09835"
     CDS             complement(4475..5692)
                     /locus_tag="VF720_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879699.1"
                     /translation="MKRMFLIGIVLGAALVGCGRDDNPSVNSRIDVPTAAIPEGVDEG
                     CNNLPVGDEFERVDNYTPEFSDPADIDNPLFPVGVLDRATSVGVEDGQVVRTETTRLK
                     GTKTVTLDNGTRLPTVRVRTIEWRDGNLAEVTTDYYAQDDIGAVWYLGEDVAHYEDGR
                     LVDNEGSWLAGPDVSPGMVMPANPQLGDVWRPENNCGLAFEDVTLLEEDVTVEGPRGT
                     ITNAVRLRVLDMDGTVEEKIFAPGYGELSAGTDPDIVSTSVAIPADRLPGATPEAIRS
                     MYGYAEFLANFAAFRRWPGLADIAAKLRTRFEAYQATGIPPLLEAAMFDALDDLDSAI
                     GGQDATAAIQAAIDIEMALLDLRLPYENSWVIDVARIKVWERRLALDRHLGDTDAVRS
                     DLATIEALKKLLS"
     gene            complement(5705..6076)
                     /locus_tag="VF720_09840"
     CDS             complement(5705..6076)
                     /locus_tag="VF720_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879700.1"
                     /translation="MTATMAMAQGLYFTLTAVWPLVHLRSFLLVTGPKTDLWLVRTVG
                     ALLAVIGITLLLLARVPEGHPSGVRTATAALGAGSAAALLLIDIIYVTRRVIRPIYLA
                     DAVAEFLLIVGWCMALAVDHE"
     gene            6203..6397
                     /locus_tag="VF720_09845"
     CDS             6203..6397
                     /locus_tag="VF720_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879701.1"
                     /translation="MTTCLDKTLKREIEVDGAAYVVTLSPDGLKLTLKGRRKGVELAW
                     KDLVSGEAALAAALRASLEE"
     gene            complement(6465..7334)
                     /locus_tag="VF720_09850"
     CDS             complement(6465..7334)
                     /locus_tag="VF720_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879702.1"
                     /translation="MSSRQSTYRERDERGRFMSDDDDDRRGGSRYGSSGSSGRSRDYD
                     DDDDRGGRMSGRGRGQGGWFGDSRGHSEAARRGWEDRDDNRSSRGGRDDDYRSYRGGR
                     DDDDRYDRGYSSRGRDRYEDDDRGSRGRGGNGRSQGGWFGDPEGHSEAARMGWENREG
                     GGRGGRGGRDDDDRYDRGYSSRGRSSSRYEDDDRGGRSGGRGQGQGGWFGDPEGHAEA
                     ARRGWREREDYRGGMSTRSRGRDYDDDDDDRGGRSRSGGGGGNGRSRGHGGWFGDLEG
                     HAEAARRGWEDRR"
     gene            complement(7705..8295)
                     /locus_tag="VF720_09855"
     CDS             complement(7705..8295)
                     /locus_tag="VF720_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7879703.1"
                     /translation="MKSAEERLETLEDLFKEELKDIYSAEQQIIKALPKMIDGSDSED
                     LRAAFTDHLEVTREQARRIEEIMSEMGDDPKGKKCEGMEGLLKEGDELLQMEGSPAVI
                     DAGLIGAAQRVEHYEIAAYGTARAHAMELGMDDAAELLQQSLTEEEEADQTLSAIAER
                     GVNMEAEEGEEAGASRSSSRSSGSGTRRAAASSKKK"
     gene            complement(8479..8676)
                     /locus_tag="VF720_09860"
     CDS             complement(8479..8676)
                     /locus_tag="VF720_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879704.1"
                     /translation="MFNERRNDRDDDDNRSSSNQGGSNRRDNDNDRGGRGTGGGSQGG
                     SQATGKSGSSGSQGQGGSKKK"
     gene            8947..11964
                     /locus_tag="VF720_09865"
     CDS             8947..11964
                     /locus_tag="VF720_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7879705.1"
                     /translation="MRARPFPFAFLALSLACLAGSNDPALARNFTPVQEGFQPAGKEV
                     PAFMADRVLVKFRESAIGASRIPPGLDYRGEVPGLATGLSRVDAAIRAARGKALRQAY
                     IRPANAARARELGVDRWFVLETDGTRPADVVAAEFAALPEVETASVDWIAFPAVVPAD
                     PLHAMHWGHNNTSQLLSYNWANNNHETGSPVGTAGFDCNAQAAWDGTQGYGSSSVIIA
                     IIDSGVAAGHPDLTQVTGYDYGSNDSNPDDNSSQAGHGTACAGVAAARANSLGSAGAA
                     PGCSIMPLKVANNAGTMSFTSIQNALYYAADHGASIISMSLGAAITSDPATDTAINYA
                     WNAGCVILAATGNENSSTISYPANHANVIGVGAASPCGDRKRSSSSSGEVNPGVSTDP
                     NGYTCDGERWWGSNYGSTTQNAAGAVDVIAPTILPTTDRLGSAGYDPSDYSKWFNGTS
                     CATPYAAGVCGLIRSKNPSWTNAQVRDQIKNTAIDATSIESAAGWDRYTGFGMVDAQA
                     AVGGGAGGDVVTVTAPNGGESLTAGVATTITWTWSGSFTTVNIDYSTNGGSSWTSIVT
                     ATANDGSHSWTVPSAATTQGRVRVSGGTATDMSNANFTITVPGGGYATLPYATGFETG
                     ALDSYWTTAVTGDGRVRVLNTNTPHGGSWHLVMDDATSGTYNQTEARLKLNLSGVTQA
                     NMTFWWKDLGDETHTQDGIYFSNNGGTSYTKVYSLNGASYSDNTWRSFTLDVDALAAS
                     AGLSLTSTFVVKFQQYDDYPVTTDGFAFDDISVTVPTGGGGITAESESNNTSGTADGP
                     VGTGLGVTGAISSSTDVDYFYFDVTSAGNVNISLSIGSSADLDWFLYNSALTEVARGY
                     TTANPEAGSYSAAAGRYYLMVDGYQTATSSYTLTITGGLANSIQPIGAAEKPWPLAPI
                     VAALHQNVPNPFHSSTAIRFALPAPGRVSLQVFDVSGRLVSTLADGILEAGEHSIDWS
                     GRNQYGEEMPIGVYFYRLTAPDFVETKKMLYTR"
     gene            12041..13582
                     /locus_tag="VF720_09870"
     CDS             12041..13582
                     /locus_tag="VF720_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX7879706.1"
                     /translation="MALLALCSLLALLAGVIPAQARDASPTVPLDDPVYERIDRLDAL
                     GKLHEDVLGMRPWSRLEIARLLARAGESAPWDTLAAWIDGARFGIAASDSRPDTIRQD
                     NGLGSIEGVVEPLVAGRDGVAFAHGVTFALGSSHSAALNRHVVAGARFDARLRIDDAS
                     GEFGYDFEGDVSILALYGRATFGNLVFQAGRDALAWGAAPGGGLLLARNTRPLDAAFL
                     TSESAWRLPWIFRHLGPTRMGLAVAALGADRTFAHSFLVATRGSFRLHSRFELGIWDT
                     LILGGEGAPKPTLAEIFHEIFPFGRGGLDEDFSDHRIAIDTRWQVVPGRLVLYHESFL
                     EDGFRPAPFGDAWDVMGLTVGVYLPSLGASGDWDCRIAATHVPAVAYRHGRWISGYTL
                     EQRLIGDELGPDAAGLSLQVARHRNDGGVTRFLLALEERNADTWAQEPAAEGAFGDIY
                     RVEDFEADRRLRGGLMIRRSMSAGVRVRGSASLEHRFDGGFPHHSGSTHYFAELGLEF
                     FSPPR"
     gene            13714..>14362
                     /locus_tag="VF720_09875"
     CDS             13714..>14362
                     /locus_tag="VF720_09875"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7879707.1"
                     /translation="MPNCLPRGAALLRRTPLLLLLLFLLSPRPVRAQLDGVHGTIGPY
                     AGWTHWDSGTNLKDKPVYGARLGVMFGKYLGLEGTYGWIPAETLYGPTIYSPRDKTNL
                     FAVGAGLHHVGADVILVLPLGRIAPYALGGYQRLIYDSDQESAPEADDDISGPELALG
                     LMSYVKPNLALRAEVRDVSHSFDQADALDDARQDNIFFTVGIQYGFGGHVESHDMD"
BASE COUNT         2448 a         4885 c         4702 g         2327 t
ORIGIN      
        1 cgccgccagc agctcctcgt gggtaccttg cgattccacg gtgccgttgg ccaggaccag
       61 gatccggtcg gccatccgca cgctcgagaa acggtgcgaa atgagcacgg cggtgcgccc
      121 ggccgtcagc tccttgaagc gctggaacac gtcgaactcg ctgcgggcat ccagcgcggc
      181 cgtcggttca tccaggatga ggagctgggc gcgccgcatg tacgcccggg cgatcgccac
      241 cttctgccac tcgccccccg acaggtccac acccttccgg aatcgcttgc cgatgatctg
      301 ctcgtaaccg aacggcaacc ggtcgatcac ctcgtcggcc aggccgtgat gggcggcctc
      361 ggcaatgcgc gcccggtcct cgcgggcctc gatccggccg accgcgatgt tctctccggc
      421 agtcaggtgg tatcgcacga aatcctggaa gatgacgccg atgttggcgc gcagttcctc
      481 gagtgagtac tccttgagat cgtgtccgtc cagcaggatg cgcccctcgt cggggtcgta
      541 caggcgcgcc agcagtttca ccagcgtggt cttgccggca ccgttctcgc cgacgagcgc
      601 cagcacctcg ccggcctgca gcgtgaagtc caggtttcga acagcccagc gatcgctccc
      661 cggataactg aagccgacgt tttcgaacgc cacgccgcgc cggatcggcg ccgggaaggg
      721 gcggggattg gccggcgagc ggatctccgg ccggatttcg aaaaaggaaa agaggtcctc
      781 caggtagagc gcctgtccag ccgtctgcga aaagccgatc aggatctctt ccagcagagt
      841 gcggagtcgc cggaacgatc ccgccaggaa ggtcaggtcg cccaccgtgt cgtggccggc
      901 cacggtgcgc catatgatga cggcataggc cacgtaatag ccgatggtac cgagcgtggt
      961 gaacaggccg ccccatcccg cccgcttcag ggccaggcgg cggttggcct ggtagaagct
     1021 gtcggccaac tcgcgatagc gatcgatcag gaagccgtgc aaaccgaaaa tcttcacttc
     1081 cttggccgtc tcgacactgg cgccggtctg tcgcacgtaa tcgagctccc gctgctcggg
     1141 cgtgcgcgaa tgggtcaagg cgtagctgcg cgcattgaag tgcgcctccc cgaggaacgc
     1201 cggaacgagc gccaccagga ggaggacgat gagccatggc gcgtagatgg ccagccccgc
     1261 accgaagccc aggatcgtca cgatgtcctg ggcctggccg aagagctggc tggtcaacgt
     1321 catccggctg gaggtctgtc ggcgcgcccg ttccaacctg tcctgtaatt cgctgtcctc
     1381 gaagtcctcg aggtcgagcg tggctgcgtg ttgcatcaga cgaacgctgg tcacgttgga
     1441 gaactgttcg gccagcagcg agtcgagcag gctgaccacg cggcccagta cgtcggcgaa
     1501 taccgccagc gcaaactcga ggaagaccag gccgatgagg cgatcggcgt gtcccccctg
     1561 gagccaggcc ccgaaggaaa ccccggccgc cggcgtggag gcctgcgcga tcacctcgtc
     1621 gatgatgagc ttgccgacgt acagcgtaag cacggggagg agcgcacgga tgagccgcag
     1681 cgcgagcgag cccagggtga gccccgggtt ggtacgccac accatggcca ggaaaggagg
     1741 gaggttgcgc agcgccccca accgttctcg caacgattgg ggcgggggga tcgggatggc
     1801 gggggacatg ccgttcatgg tggcaggaac cgatcccgac gtcgtggggg aattcccggt
     1861 gccgaccgac cggaagtgcg acaatccccc gagctattgg gtcgattggc agtgcccggt
     1921 acagccgaca acctggagtg cgatcaatcg tccgggcgag agcggtcgtc gctgagtgtt
     1981 cctcaccccg ccggcgccca gcgggcctgc gcaatcgcac gccccatgat ccagcgcccg
     2041 agcagcacaa tgaccgcttc caccgccagg aaaccgccga acacctccct gcgccagcaa
     2101 agcgcggcaa cgccggccac catggcggct gccgcgaaga gggcgattag gacgatggtg
     2161 cgagcaatct gggttccctc gtccaccggg cgggagaggg ggacggcgtg gccgttgatg
     2221 cacgggaaga gcgtaaggat gggcgatagc aggaggcccg gcagcaggag gagcaggtgc
     2281 atgggatcgg agtcgaggag tgcgatggcc gccgcgagcg cgagcaggac cgggaaaccg
     2341 aatagcaggg tcacggcccg gcgcgcccca aggcagaacg gggccggccc ggggagggga
     2401 gccgctcgaa acagcatggc cgccggggcc tgggacgagt ggcgcaacgt gtcgagcgcc
     2461 agcatgggga tcaggccgac gtacatgccg gcgaatgcca ggctgaaggc accgaagccg
     2521 gtcgacttca tgttttccag cagcgtgagc gccgggatca cgatcatcgg aacgaccccg
     2581 ggatacacgc gcaaccgtgt ttcgcgatcg cggaagaggt aggccagggt gagcgcgaaa
     2641 gcggcgcggg gcaccgagcc gcccacccac cagcgtagcg gcgggtgctc gacgagggcg
     2701 tgaagccagc gccgcggcgg acggctgctt cgggcaggtg acgaggccac cgccgtctgc
     2761 aatcccgccg cgtaggctcc ggccagccgc ccgagcgcca gccacagcac gaggagcgcg
     2821 gcgccgacgg ccaggccggc caccgcccac gagcggatgg cgccggaacc ggccagcgca
     2881 tcgtcaaagc cggcgaacca gacgggtggc agcgccagtc cccaggccgg catttcccgg
     2941 atcgtgtgtg cgccggtctt cccgatgacg cgcgggccga actgcgaggc caggacgatc
     3001 aggatcgaga agccgacctg caccgtcgtc agcaggcttt cgaggcgttc gcggccgatc
     3061 cacctgaggc agagctcgta actcaatacc acgatgccga ccgtgaagag cgccgagagt
     3121 ccggtggaga cgaggtgaac gaacgggaag cgccagtcgc tgtccggagc gaaggtgccg
     3181 acaagcaatc cgaaaaggtt gagcgccccg gcgatccaga aggccacttc gagcatgacc
     3241 cggactttgg cacggagcac gttgccggcc tccaccggcc ggtgcatcag gatgtcgggc
     3301 tcctcgcggt tgaacaacgt ctcgccggcg gtggtcatca ggaagatggc caggcagaag
     3361 aacgagaagc cgtgcagcat ggccgaaagc accaggaccg gttgcccgat tccggcgaag
     3421 gccacgatgc cgaatgccgt gtacgacagc agcacccaga agaggcgttc acccacggat
     3481 ttcggggtcg aggccttgcc acgcgccacc gcggtgcggc cgcgcacgaa cagggtcagg
     3541 aagagacggc ggagcgcctt gtccgcgagc ggggtgtcgc gggtcgcccc tgtcacgtcc
     3601 ggaaggtccg cgcgaagtcc tcggcccggc gctccagatc ggtgtttccg gtcaactgcg
     3661 tgaagagccg ttcgagagtt tcggcgccat gggcggcgat gagcgcgtcg ggagcgccgt
     3721 ccaccaggag cttccccttg tcgatgatga tcacgcgatc gcacacacgc tcgaccacgt
     3781 cgaggatgtg cgagctgtag agaatcgtct tcccctcgcg ggccagcgac tggatcagcg
     3841 ccttgaagcc gaccgcggcg ttggcatcca gcccctccag cggttcgtcg aagaagacca
     3901 ccggcgggtt gtggagcaga gccgaagtga tgaccacctt gcgccgcatt cccttggaat
     3961 aggcgccgag ctgcttgtcg gtgagcgtgt cgaatcccag gtcgaagaag gccaggaact
     4021 ggcggatgcg ggcttcggcc tgatccgccg ggatgtcata gagcgccgcc accatttcga
     4081 gatattcgag gccggtgagt gattcgaaca cggctcccga gtcaggcacg aagccgatgc
     4141 ggcgcttgac ctcgatcgat tgggtgagaa ggtcgaactc cccgatcgtg gccgtgccgc
     4201 tcgtcggctc gagaagcccc gtgagcatgc gcagcgtggt ggtcttgccg gcaccgttcg
     4261 gaccgagaaa gccgacgatc tggcctgccg gcaccgaaaa cgacaggtcg tccacggcga
     4321 tctgggtgcc gaacgtgcgg gtcaggtggt cgaggacgat catcgggaga tgatatcgga
     4381 cagcgagtct ttgcgggcgg ggggacaccc gtgccggcac ccgccccggg ggagcgcgcg
     4441 ggagttgccg acgcgctccc ccggaggagg ctcgctagga aaggagcttc ttcagcgctt
     4501 cgatcgtggc caggtcactc cgtacggcgt cggtatcacc caggtgacga tcgagcgcca
     4561 gccggcgttc ccacaccttg atgcgcgcca cgtcgatcac ccagctgttc tcgtacggca
     4621 gccgaagatc caggagcgcc atctcgatgt cgatggccgc ctggatcgcc gctgtcgcat
     4681 cctgtccccc gatcgccgag tcgagatcgt cgagcgcgtc gaacatcgcc gcctcgagca
     4741 gcggagggat acccgttgcc tggtaggcct cgaaacgggt ccggagcttg gccgcgatgt
     4801 cggccaggcc aggccaccgg cggaacgccg cgaaattggc caggaattcc gcgtacccgt
     4861 acatggagcg gatggcttcg ggagtggcgc ccggcagccg gtccgcgggg atggcgaccg
     4921 atgtgctcac gatatcggga tccgttcccg ccgacagctc gccatagccg ggcgcgaaga
     4981 tcttttcctc cacggttccg tccatgtcga gcacgcgcag gcgtacggca ttcgtgatcg
     5041 ttccccgcgg accctcgacc gtcacgtcct cctcgagcag cgtcacatcc tcgaaggcaa
     5101 gtccgcaatt gttctcgggg cgccagacgt cgccgagctg cgggttggcc ggcatgacca
     5161 tgccgggcga aacgtccggg ccggccagcc agctcccctc gttgtcgacg aggcggccgt
     5221 cttcgtagtg agccacatcc tcgcccaggt accagaccgc cccgatgtca tcctgcgcgt
     5281 agtagtcggt cgtgacttcc gccaggttgc cgtcacgcca ctcgatggtc ctcacacgga
     5341 ccgtcggcag gcgcgttccg ttgtccagtg tgacggtctt cgttcccttg aggcgggtcg
     5401 tctcggtccg cacgacctga ccgtcctcga cgcccacgga tgtggcgcgg tcgagcacgc
     5461 cgactgggaa gaggggattg tcgatgtcgg ccggatcgct gaattcgggc gtgtagttgt
     5521 cgacccgctc gaattcgtca cccaccggga ggttgttgca tccctcgtcc acgccttcgg
     5581 gaatggcggc ggtcggtaca tcgatgcgcg agttgacgga cggattgtcg tcgcgcccgc
     5641 atcccaccag ggccgcgccg agcacgatac cgatcaggaa cattcgcttc attgggggat
     5701 ctccttattc atgatcgacg gccagcgcca tgcaccagcc aacgatgaga agaaattccg
     5761 cgacggcgtc cgccaggtag atcggtcgga ttacgcgccg ggtgacgtag atgatgtcga
     5821 tgagaaggag cgcggcggcg cttcccgccc ccagggctgc ggttgccgtc cgcacgccgg
     5881 atggatgccc ctcgggcacc cgagccagga ggaggagcgt gattccgatc acggccagca
     5941 gggcgcccac cgtgcggacg agccacaagt cggtcttcgg gcccgtgacc aggagaaagg
     6001 aacgcaggtg aacgagtggc cagaccgcgg tgagcgtgaa ataaagcccc tgggccatcg
     6061 ccattgtggc cgtcaccgcc ggaaaccccg attcccgccg cctctttggc ggtaggctgg
     6121 tgggaactcc atgcacaaaa ggagaggcac gaggaagctc gaccctccaa aagtgcctga
     6181 ttcccatgag gctagcgccg acatgacgac atgcctggac aagacgctga aacgcgaaat
     6241 cgaggtggat ggcgccgcat acgtggtgac gctctctccc gacggcctga agctcacgct
     6301 caagggacgt cgcaagggtg tggagctggc ctggaaagac ctggtgagtg gagaggcggc
     6361 cctggccgcg gccctccggg cttcgctcga ggagtgaccc gggggggacg cacgacgatc
     6421 catgcgtccc cccggatcac gacttcagtc ggagccggcc cgggtcagcg ccggtcttcc
     6481 caaccacgac gggccgcttc ggcgtgtcct tccagatcac cgaaccagcc gccgtgcccg
     6541 cggctgcgcc cgttgccgcc accgccgccg ctgcgggaac gcccgccgcg atcgtcgtcg
     6601 tcgtcatcgt aatcgcgacc gcgggaacgc gtggacatgc cgccacggta atcctcgcgc
     6661 tcacgccagc cacggcgcgc ggcttcggcg tgcccttccg gatccccgaa ccagccgccc
     6721 tgtccctggc cccggccacc gctgcggcca ccacgatcat cgtcctcgta acggctggag
     6781 gagcgaccgc gcgaggagta gccccgatcg tagcgatcgt cgtcatcgcg accgccccgg
     6841 ccgccccggc cgcccccttc acggttttcc caacccatcc gggccgcctc ggagtgacct
     6901 tccggatctc cgaaccagcc gccctgcgag cggccgttgc cgccccggcc acgtgagccg
     6961 cgatcgtcgt cttcgtacct atcacgacca cgggaggaat aaccccggtc gtaccggtcg
     7021 tcgtcatcgc gcccaccccg gtaggagcga tagtcatcat cgcgcccacc gcgggaggaa
     7081 cggttgtcgt cccggtcttc ccagccgcga cgagccgctt ccgagtgacc gcgcgagtcg
     7141 ccgaaccagc caccctggcc ccgcccgcgg ccgctcatcc gtccgccccg gtcgtcatcg
     7201 tcgtcgtagt cgcgcgaacg acccgaggac ccggacgatc cgtagcgact tcctccgcga
     7261 cggtcatcgt cgtcgtcact catgaatcgg ccgcgctcat cacgctcccg atacgtactc
     7321 tgcctgctgc tcatcgtgaa cctcctgttc gagtgacttg ccaggcggca agtggactcc
     7381 gggagtgccc gacacgggcg tgtcgttccg gagcggtgtt cgaaagcgca agaggagtgc
     7441 caagcgcgcc gtggctgcga tgggttgcat tgcatcggaa tggatcgccg gagaggggga
     7501 gcggcggagg cttgcgcaag ttgttccgac ggggcggcgg taaatccctc cgcgcaccac
     7561 attccttcac atgagggatc tcatgcagga cgactgggtg aaacaaggat gcagattgaa
     7621 acaggatcaa gatgaaaatg agcagcgacg gcgatcggtc ccgcttcttc ccggaaccat
     7681 cgccgtcgct cgacgtgccg tggcttactt cttcttgctc gaggcggccg cgcgacgcgt
     7741 gccggaaccc gacgagcggg acgaggaacg ggatgccccg gcctcctcgc cttcctcggc
     7801 ctccatgttc acgccccgct ccgcgatggc ggaaagggtc tggtcggctt cctcttcctc
     7861 ggtcagcgac tgctgcagga gttcggcggc gtcgtccatg cccagttcca tggcgtgcgc
     7921 ccgggcggtg ccgtaagccg cgatctcgta gtgctccacc cgctgcgccg cgccgatcag
     7981 gccggcatcg atcacggccg ggctcccctc catctggaga agctcgtcgc cttccttgag
     8041 gagtccttcc atcccctcgc acttctttcc cttagggtcg tcccccatct cggacatgat
     8101 ttcctcgatc cggcgcgcct gctcgcgcgt cacttccagg tgatcggtga aggcggcgcg
     8161 caggtcttcc gaatccgagc cgtcgatcat cttcggcagg gccttgatga tctgctgctc
     8221 ggcgctgtag atgtccttga gctcttcctt gaagaggtcc tcgagtgtct cgaggcgttc
     8281 ttcggcggac ttcatggctg tctcctctcg gtggtctgtc gttatgacgc ggtcccggca
     8341 tgggtgggcc gggccacgat gctgaaaacc gagccagcaa ggtgcgagcc aaccggaccg
     8401 agtcatccac tggggctctc cggaacagga acgccggcag ggactgccgg cgctccgatc
     8461 gcccattacc ttcccgactt acttcttctt cgatccgccc tgaccctgcg atccggacga
     8521 tcccgacttt ccggtggcct gtgaaccgcc ctggctgcca ccgcccgttc cccgcccccc
     8581 gcgatcattg tcgttgtcgc gccggttcga accgccctgg ttggagctcg agcgattgtc
     8641 gtcgtcatca cgatcgttgc gccgttcgtt gaacattctg cactccttcg tttcgacgct
     8701 cattgaggag cgtcctggtt gatcactctg gccgtgttga aggacgagca acccatacgc
     8761 cacgaaaccg ttttcaaggc ggtaatgttt tccgctattg gaattgtcgt gacaaaccgc
     8821 agcgcgctgt gataccacaa caaccgcgca tgcttggtcc ccccccaaca aggccgcgcc
     8881 caacggccaa cgcatgccat gcctgaagtg ccagcaccgg tacccgttca gatgcatgga
     8941 ggatttatgc gcgcacgtcc gtttcccttc gcgttccttg ccctctccct ggcctgcctg
     9001 gccggcagca acgatccggc gctcgcccgg aatttcacgc cggtccagga gggattccag
     9061 ccggccggga aggaggtgcc ggccttcatg gccgaccggg tgctggtgaa gttccgggaa
     9121 tcggccatcg gagcctcacg cattccgccg ggcctcgact accgcgggga agtgccgggc
     9181 ctggccaccg ggctttcccg ggtggacgcc gccatccgcg cggccagggg gaaggcgctc
     9241 cggcaggcct acatccgtcc tgccaacgcg gccagggcgc gggagctcgg cgtggatcga
     9301 tggttcgtgc tcgagacgga cggcacccgg ccggccgacg tggtggcggc cgaattcgcg
     9361 gcgcttcccg aggtggagac ggcctcggtc gactggatcg ccttcccggc cgtcgtgccg
     9421 gccgaccctc tccacgccat gcactggggg cacaacaaca catcccagct gctcagctac
     9481 aactgggcca acaacaacca cgaaaccggc tcgccggtcg gcacggccgg cttcgactgc
     9541 aacgcccagg ccgcctggga tggcacccag gggtacggat cgagctcggt catcatcgcc
     9601 atcatcgact cgggcgtggc cgccggccac ccggacctca cccaggtgac cgggtacgac
     9661 tacgggagca acgactcgaa cccggacgac aactcctcgc aggccggcca tggaacggcg
     9721 tgcgccggcg tggcggcggc ccgggccaac agcctcggct cggcgggggc cgcgcccggc
     9781 tgcagcatca tgccgctcaa ggtggccaac aacgccggca cgatgagctt cacctcgatc
     9841 cagaacgcgc tgtactacgc cgccgatcac ggcgcgagca tcatctcgat gagcctcggg
     9901 gccgccatca cctcggatcc ggccaccgac acggccatca actacgcctg gaacgccggc
     9961 tgcgtcatcc tggccgccac cggcaacgag aactcgtcca cgatcagtta tcccgccaac
    10021 cacgccaacg tgatcggcgt gggcgccgcc tcaccctgcg gcgatcggaa gcgctcttcc
    10081 agctcgagcg gcgaagtgaa cccgggcgtc agcaccgacc cgaacggcta cacctgtgac
    10141 ggcgagcggt ggtggggttc caactacggc agcaccaccc agaacgccgc cggggccgtg
    10201 gacgtgatcg cgccgaccat cctgccgacc acggaccggc tgggctcggc gggctacgat
    10261 ccgagtgact attcgaagtg gttcaatggc acctcgtgcg ccacgcccta cgcggccggc
    10321 gtctgcggcc tcatccgctc gaaaaacccc tcgtggacca acgcccaggt gcgcgaccag
    10381 atcaagaaca ccgccatcga cgccaccagc atcgagtcgg ccgccggctg ggatcgctac
    10441 accgggttcg gcatggtcga tgcccaggca gcggtcggtg ggggcgccgg cggggacgtc
    10501 gtcaccgtca ccgcgccgaa cgggggggag agcctgacgg ccggtgtcgc caccaccatc
    10561 acctggacct ggagcggcag cttcacgacg gtcaacatcg actattccac caatggcggt
    10621 tcgagctgga cgagcatcgt gaccgccacc gccaacgacg gctcccactc gtggaccgtc
    10681 ccctccgcgg ccaccacgca ggggcgcgtc cgcgtgtcgg gcggcaccgc caccgacatg
    10741 agcaacgcca atttcacgat caccgtgccc ggcggcgggt atgccacgct tccctatgcc
    10801 acgggcttcg agaccggtgc gctcgacagc tactggacga cggcggtcac cggcgacggc
    10861 cgcgtgcgcg tcctcaacac caacacgccg cacggcggtt cgtggcacct ggtgatggat
    10921 gacgccacga gcggcactta caaccagacg gaggcccgcc tcaagctgaa cctttccggc
    10981 gtcacccagg ccaacatgac cttctggtgg aaggatttag gcgacgaaac ccacacgcag
    11041 gacggcatct acttctcgaa caacggcggt acgagctaca cgaaggtgta cagcctgaac
    11101 ggcgccagct acagcgacaa cacctggcgc tcgttcaccc tcgacgtgga cgccctggcc
    11161 gcctcggcgg gtctttcgct cacctcgacg ttcgtcgtca agttccagca gtacgacgac
    11221 tacccggtca cgaccgacgg attcgccttc gacgacatct ccgtcaccgt tccgacgggg
    11281 ggaggcggca tcacggccga gagcgaatcg aacaacacct cgggcaccgc cgacggcccc
    11341 gtcggcaccg ggctcggcgt aaccggcgcc atctcgtcat cgaccgacgt ggactacttc
    11401 tacttcgatg tcaccagcgc cggcaacgtg aacatctctc tgagcatcgg gagctcggcc
    11461 gacctcgact ggttcctgta caacagcgcg ctcaccgagg tggcccgcgg ttacaccacg
    11521 gccaacccgg aggccggcag ctacagcgcc gccgccggcc ggtactacct gatggtggac
    11581 gggtaccaga cggccacgag cagctacacg ctcacgatca ccggcgggct ggccaactcc
    11641 atccagccca tcggcgcggc cgaaaaaccc tggccgctcg cgccgatcgt ggcggcactc
    11701 caccagaacg ttcccaatcc tttccattcc tccacggcca tccggtttgc ccttccggcg
    11761 ccgggacgcg tgtctctcca ggtgttcgac gtttcgggcc ggctcgtctc cacgctggcc
    11821 gacggcattc tcgaagccgg ggagcattcg atcgactggt cgggccggaa ccagtacggg
    11881 gaggagatgc cgatcggcgt ctacttctac cggctgacgg ctcccgactt cgtcgagacg
    11941 aaaaagatgc tgtacacgcg ctagattcaa ccggcggcgg cgccgatggg tgccgccgcc
    12001 tttcattgag cgggatcggg gggcggttca taatcgattt gtggccctcc tcgcgctttg
    12061 ttccctcctc gccctccttg ccggcgtcat tccggcgcag gcccgggatg cctcgccgac
    12121 cgttccgctc gacgaccccg tctatgagcg catcgatcga ctcgatgccc tgggcaagct
    12181 ccacgaagac gtgctcggca tgcgcccatg gtcgcgactc gaaattgcgc ggctcctggc
    12241 ccgggccggt gagagcgccc cctgggacac gctggcggcc tggatcgacg gcgcccggtt
    12301 cggcattgcc gccagcgact cccgtcccga cacgattcgc caggacaatg gcctcggaag
    12361 catcgagggg gtcgtcgaac cgttggtggc cggccgggac ggagtggcat tcgcccacgg
    12421 cgtgacgttc gcgctcggca gctcgcactc ggcggcgctc aaccgtcatg tcgttgccgg
    12481 cgcccgcttc gatgcacgcc tcaggatcga cgatgcttcc ggcgagttcg gctacgactt
    12541 cgagggagat gtgtcgatcc tcgcgcttta cggccgggcc accttcggca atctcgtgtt
    12601 ccaggccggc cgcgatgccc tggcgtgggg cgcggctcca ggcgggggac tgctgctcgc
    12661 ccggaacacc cggcccctcg atgcggcgtt tctgacctcg gagtcggcct ggcgcctgcc
    12721 atggatcttt cgccacctcg gcccgacgcg catgggcctc gcggtggcgg cactgggcgc
    12781 cgatcgcacg ttcgcccatt ccttcctcgt ggcgacgcgc gggtcgttcc ggctgcattc
    12841 ccgtttcgag ctcgggatct gggacacgct catcctcggt ggggaggggg ctcccaaacc
    12901 gacgctcgcc gagatcttcc atgaaatctt tcccttcggt cgcggcgggc tcgacgagga
    12961 tttcagcgat caccgcatcg cgatcgatac gcgctggcag gtggtgccgg ggcgcctggt
    13021 gctgtaccac gagtcgtttc tcgaggacgg ctttcgcccc gcgccgttcg gtgacgcctg
    13081 ggacgtgatg ggcctgaccg tcggtgtcta cctgccgtcg ctgggcgcct ccggcgactg
    13141 ggactgtcgg atcgcggcca ctcacgtgcc ggcggtggcc taccggcatg ggcgctggat
    13201 cagcggctac acgctcgaac agcgcctgat cggcgacgaa ctgggcccgg atgccgccgg
    13261 cctgtcgctc caggtggccc gtcaccggaa tgacggcggc gtcacgcgct tcctcctggc
    13321 cctggaggag cggaatgccg acacgtgggc ccaggaaccg gcggcggaag gtgccttcgg
    13381 tgacatctat cgcgtcgagg atttcgaggc ggatcgacga ctccgcggtg gcctgatgat
    13441 tcgccgttcg atgtcggcgg gagtgcgggt gaggggcagc gcctcactcg agcatcgctt
    13501 cgatggggga ttcccccatc attccggatc gacacactat ttcgcggagt tgggattgga
    13561 gtttttctcg ccacctcgtt gaccgaagcg cgtcgggagc gcacgatcag aacgggtaca
    13621 ggtatgggtc gcatttcgaa acagtcgggc cgttgatgaa taggacgcgg ggaatgagcc
    13681 gcgagcattg cccccaactc gacgaggtgt cacatgccga attgcctgcc gcgtggcgca
    13741 gccctgctgc gtcgcacccc gctgctcctg cttctcctct tcctcctgtc accccggccg
    13801 gtccgggccc agctcgacgg ggtccacggc accatcggcc cctacgccgg ctggacccac
    13861 tgggactccg gcaccaattt gaaagacaag ccggtttacg gcgcccgcct cggggtgatg
    13921 ttcgggaaat atcttgggtt ggagggaacg tacggctgga ttccggccga aacgctttac
    13981 ggcccgacga tctacagccc ccgtgacaag acgaacctgt tcgcggtggg cgccggactc
    14041 caccacgtcg gcgctgacgt gatcctggtc cttccgctcg gcaggatcgc gccttacgcg
    14101 ctgggcggat accagaggct catctacgac tccgaccagg agtcggctcc ggaggcggac
    14161 gatgacattt ccggcccaga gctggcgctg ggcctcatga gctacgtgaa gccgaacctg
    14221 gcgctccgtg ccgaagtgcg cgacgtgagc cactcgttcg accaggccga tgccctggac
    14281 gacgcccgcc aggacaacat cttcttcacc gtcggcatcc agtacgggtt cggcgggcat
    14341 gtcgaatcac acgacatgga cg
//
LOCUS       DASVOD010000430         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_133874, whole genome shotgun sequence.
ACCESSION   DASVOD010000430 DASVOD010000000
VERSION     DASVOD010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133874"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="VF720_09880"
     CDS             complement(<1..935)
                     /locus_tag="VF720_09880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879708.1"
                     /translation="MTLVVERAPNPLAWGALIAHDPNATIFHEPDFHAALLASYPEFQ
                     SHHLWWKDDAGRLVGGLPTLRVVRAGMSQVLSLPFGSYGTPLVGVHAGEEPGRIRGVL
                     VDAWRTEATRSGVVRAHLVPYAPESGDPARGRLSEEWRRGERTHVIPLTEGFDAIWFG
                     RYDKENRTAARKAVRAGVVVAEEPDSAEVLEALYRRQAREWTGHALYRYGLFRELIDR
                     LGDRARLWVARKDDAPLFAVMAFYHRGTVTPWVSGAAPEARGTSAGNLIHKVIIEDGC
                     RRGFERYNFGGSGGVPGIEAFKVAFGGEPVDYHSW"
     gene            complement(932..>2036)
                     /locus_tag="VF720_09885"
     CDS             complement(932..>2036)
                     /locus_tag="VF720_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7879709.1"
                     /translation="GRETVELDVDLVRGAYRGKRLLVTGAGGSIGSEICRHLLAAEPA
                     ELVILGRGENSIFEADLSFKERAVTTGGAVTRIDTVIGDVRDTALVERLLARVKPHAI
                     FHAAAHKHVWLMEQHPAEAVANNVLATAGLVDAAVRHEAERFVLISTDKAVNPHAVMG
                     ATKRLAEIHLLQRAAELKGSGSGTRLMAVRFGNVLGSRGSVVPIFQRQIGRGGPVTVS
                     HPEAARFFMTIKEAALLVIEAGAIGQGGEIFVLDMGEPMSIAELARDLIILSGLRPDV
                     DVPIAFTGLKSGEKLREGLVHDFEPLTPTAVPGIRVTRGLKPDQPAVDGAALATLRRL
                     ADEADRPGIVAAIEALVPEARLARDVPGSPAAH"
BASE COUNT          315 a          742 c          688 g          291 t
ORIGIN      
        1 aaccacgagt ggtaatccac cggctcgcca ccgaacgcca ccttgaacgc ctcgatgccg
       61 ggcacgcccc ccgagccgcc gaagttgtag cgttcgaagc cgcgccggca gccgtcctcg
      121 atgatcacct tgtggatgag gttgccggcg gaggtgccgc gcgcttccgg cgccgcaccc
      181 gatacccagg gcgtcaccgt gccgcgatgg tagaaggcca tcacggcgaa gagcggcgcg
      241 tcgtccttgc gcgccaccca gaggcgcgcg cggtcgccca gccggtcgat gagttcacgg
      301 aagagaccgt agcggtagag ggcgtggccc gtccactcgc gggcctggcg gcggtagagc
      361 gcctcgagca cctcggccga atcgggctcc tcggccacca ccacgccggc ccgcaccgcc
      421 ttccgcgcgg ccgtgcggtt ttccttgtcg taccggccga accagatggc gtcgaacccc
      481 tcggtcaacg ggatcacgtg cgtccgctcg ccgcgccgcc actcttccga aaggcggccc
      541 cgggccgggt cgcccgattc cggcgcgtac ggcacgagat gggcgcgcac gacacccgag
      601 cgcgtggctt ccgtgcgcca ggcatcgacg agtaccccac ggatccgccc gggctcctcg
      661 ccggcatgca cgccgacgag cggcgtgcca tacgaaccga acggcagcga gagcacctgc
      721 gacatcccgg cgcgcaccac gcgcaacgtg ggaagcccgc cgacgagccg accggcgtca
      781 tccttccacc agaggtgatg ggattggaac tccgggtagc tcgcgagcag ggccgcatgg
      841 aagtcaggtt cgtggaagat cgtggcgttc gggtcatgtg cgatcagcgc gccccaggcc
      901 agaggattgg gggcgcgctc gacgacgagc gtcaatgggc ggctgggctg cccggcacgt
      961 cccgggccag ccgagcctcc ggcaccagcg cttcgatcgc ggccacgatg ccggggcgat
     1021 cggcctcatc ggcaagacgc cggagcgtgg ccagcgcggc cccatccacg gccggctgat
     1081 ccggcttgag gccccgtgtc acgcggatgc cgggcaccgc ggtaggcgtc agcggctcga
     1141 agtcgtgcac cagcccctcg cggagtttct cgcccgactt gagtcccgtg aaggcgatcg
     1201 gcacgtcgac atcggggcga aggcccgaca ggatgataag gtcgcgcgcc agctcggcga
     1261 tcgacatcgg ctctcccatg tcgaggacaa aaatctcccc tccctggccg atggcaccgg
     1321 cctcgatgac gaggagcgcc gcctccttga tcgtcatgaa gaagcgggcc gcctcgggat
     1381 ggctcacggt gaccggaccg ccgcgcccga tctgccgctg gaagatcggc accaccgacc
     1441 cgcggctccc gagcacgttg ccgaaccgga cggccatgag tctcgtgccg ctgccgctcc
     1501 ccttgagctc cgccgcgcgc tgcaacaggt ggatctcggc cagcctcttg gtagccccca
     1561 tcaccgcgtg cgggttcacc gccttgtcgg tggagatgag gacgaagcgc tcggcctcgt
     1621 gccgcaccgc cgcgtcgacg aggccggccg tggccagcac gttgttggcc accgcttccg
     1681 ccggatgctg ctccatgagc cacacgtgct tgtgggccgc cgcgtgaaag atggcgtgcg
     1741 gcttcacgcg ggcaagcaat cgttcgacga gcgcggtgtc gcggacatcg ccgatcacgg
     1801 tgtcgatgcg ggtgacggcg cccccggtcg tcaccgcccg ttccttgaag ctcaaatcgg
     1861 cctcgaagat cgagttctcg ccgcgcccca ggatgaccag ctcggccggt tcggccgcca
     1921 gcaggtgccg gcagatttcc gatccgatcg atccgccggc accggtcacc aggagcctct
     1981 tgccgcggta ggcgccccgc acgaggtcca cgtcgagctc caccgtttcg cggccg
//
LOCUS       DASVOD010000431         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_81574, whole genome shotgun sequence.
ACCESSION   DASVOD010000431 DASVOD010000000
VERSION     DASVOD010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81574"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VF720_09890"
     CDS             complement(<1..603)
                     /locus_tag="VF720_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX7879710.1"
                     /translation="MGDEELQAQPAAAPPPAAAEGSLLEELIRETKMTPADEGYQKHV
                     TALGELVRTMVGGPKVDKIETKYVDAMIAELDKKLGLQLDEILHHPDVQKLESSWRGL
                     KLLVDRTNFRENIKVELLNVSKDDLQADFEDSPELVKSGLYKHVYSAEYGTFGGQPVG
                     AMIANYEFNAGPRDMALLGQCASVAAMAHAPFISSVGAKFF"
     gene            complement(606..1103)
                     /gene="tssB"
                     /locus_tag="VF720_09895"
     CDS             complement(606..1103)
                     /gene="tssB"
                     /locus_tag="VF720_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017020857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HEX7879711.1"
                     /translation="MAKEGSVAPKERINIVYKPATGNAQAEIELPLKLMVLGDFTGRA
                     DSTPMEDRKPINIDKDNFNDVMRAHNLSTDISVKNTLTGQEGDQLATSLKFSTLKDFE
                     PEAVANQVPELKKMLELRAALVGLRGPLGNVPAFKKKLEKIMGDEAAKQKLLGELGAD
                     KKEGE"
     gene            complement(1183..2748)
                     /gene="tssA"
                     /locus_tag="VF720_09900"
     CDS             complement(1183..2748)
                     /gene="tssA"
                     /locus_tag="VF720_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HEX7879712.1"
                     /translation="MPFSSAELAPLGTTPISPDRPGGESVVYEPEFEAIKAEIEKIDS
                     VSGAIPDFNNVVTLGHAILSTKSKDLTVSGYLTLGLFRTGGFPGLTGGLTLCRDLVAN
                     FWESFYPEPSRMRGRVAALSWVNDRLAGAIPKVTPTPADHEAVQAALEAYKSLDATVS
                     EKFGAEAPSFGDLRRALDDAVRMSTPTAAPAAAAPAAGGATQAATGPVTIDSPDAAYR
                     VFRNAGTEIRRAAEYLIKQDPASPVPYLAQRGAAWAPIRELPQDREGITQFNPPDSNV
                     AANWEKLMEAGEWKGLLEDTEWRLTASPFWLDINYYAALALENLGHDAAARAVTDQTA
                     VLVKRFPKLLDLMYSDGRPFASAGTRQWIQSEMSSGGGGNGMGGGGEQAPIQEDPVEE
                     AGREARGMAARGKLTEAVEMLQGRIKESGQPRERFRWRLLLARILADGNQPAVAAAQL
                     RLLDEDIEAHRLEAWEPGLALDTLVALYRCEKKLGQNGAAPGRDGLSRLDTLYNRLCR
                     LDVLTALSLDGKK"
BASE COUNT          406 a          936 c         1005 g          547 t
ORIGIN      
        1 gaagaacttg gcgcccaccg aggagatgaa cggtgcgtgg gccatcgcgg ccacggaggc
       61 gcactggccg agcagggcca tgtcgcgcgg ccccgcgttg aactcgtagt tggcgatcat
      121 ggcgccgacc ggctggccgc cgaacgtgcc gtattccgcc gagtagacgt gcttgtagag
      181 gcccgacttc acgagctccg gcgagtcctc gaaatcggcc tgcaggtcgt ccttggagac
      241 gttgagcagc tcgaccttga tgttctcgcg gaagttggtg cggtcgacca ggagcttgag
      301 cccccgccat gacgactcga gcttctgcac gtcggggtgg tggaggatct cgtcgagctg
      361 caggccgagc ttcttgtcga gctcggcgat catggcgtcg acgtacttgg tctcgatctt
      421 gtcgaccttg ggcccgccga ccatggtgcg caccagttcg ccgagggcgg tgacgtgctt
      481 ctggtacccc tcgtccgcgg gggtcatctt ggtttcgcgg atcagttcct cgagcagcga
      541 cccttccgcc gcggcgggcg gtggtgcggc ggccggctgc gcctggagtt cttcgtcacc
      601 catgatcatt caccctcttt cttgtccgcg cccagttcgc cgaggagctt ctgcttggcc
      661 gcctcgtcgc ccatgatctt ctccagcttc ttcttgaagg ccggcacgtt gccgagcggg
      721 ccacgcaagc ctacgagtgc ggcacgcagt tccagcatct ttttgagctc gggcacctgg
      781 ttggccacgg cctcgggctc gaaatccttg agcgtcgaga acttgaggct ggtggccagc
      841 tgatcgccct cctggccggt cagcgtgttc ttcacgctga tgtcggtcga aaggttgtgg
      901 gcccgcatca cgtcgttgaa attatccttg tcgatgttga tcggcttgcg atcttccatc
      961 ggcgtggagt cggcccggcc ggtgaagtcg cccagcacca tcagcttgag cggcagctcg
     1021 atctccgcct gggcgttccc cgtggcgggc ttgtagacga tgttgatccg ctccttgggg
     1081 gccactgacc cttccttggc catccgaaca cctcctgttg gcgaccggcc gtcaggccgg
     1141 tctatctctt tgactcaggc gtcactgctt ccctacgagg cgtcacttct tcccatcaag
     1201 tgacaaagcc gtcaacacat ccagtcggca cagtcgattg taaagggtgt cgagccgcga
     1261 gaggccgtcc cggcccggtg cggcgccatt ctgccccagt ttcttctcgc agcgatagag
     1321 cgccacgagc gtgtcgagcg ccagccccgg ctcccaggcc tccaggcgat gggcctcgat
     1381 atcctcgtcg agcagccgga gctgggccgc ggccacggcc ggctggttgc cgtcggccag
     1441 gatgcgggcc aggagcagcc gccagcggaa tcgctcccgc ggctggccgc tctccttgat
     1501 gcgcccctgg agcatttcga cggcttcggt gagctttccc cgggcggcca tgccgcgggc
     1561 ctcgcggccg gcttcctcga cggggtcttc ctggatgggg gcctgctctc cgccgccgcc
     1621 catgccgttg ccgccgccgc cggacgacat ctcggactgg atccactggc gggtgccggc
     1681 cgacgcgaac ggccgcccgt cggagtacat caggtcgagc agcttcggga agcgcttcac
     1741 gagcaccgcc gtctggtcgg tgaccgcccg cgccgccgcg tcgtgtccca ggttctccag
     1801 cgccagcgcc gcgtagtagt tgatgtcgag ccagaagggg ctggccgtca ggcgccactc
     1861 ggtgtcctcg aggagcccct tccactcgcc ggcttccatg agcttttccc agttggccgc
     1921 cacgttcgaa tccggcggat tgaactgggt gatcccttcc ctgtcctgcg gcagctcgcg
     1981 aatgggcgcc caggccgccc cccgctgggc caggtagggg acgggggagg cgggatcctg
     2041 cttgatgaga tattcggcgg cgcggcggat ctcggtgccg gcgttgcgaa agacgcggta
     2101 cgcggcgtcg ggggagtcga tggtgacggg accggttgcc gcctgtgtcg cgccgccggc
     2161 cgctggtgcc gccgctgcgg gagccgcggt gggcgtggac atccgcacgg cgtcatcgag
     2221 cgcgcggcgg aggtcgccga aggagggcgc ttcggcgccg aacttctcgc tgaccgtggc
     2281 gtcgagggat ttgtacgcct cgagcgccgc ctgcacggcc tcgtggtcgg ccggagtcgg
     2341 cgtcaccttg ggaatggcac cggccagccg atcgttcacc caggaaagcg cggccacccg
     2401 gccgcgcata cggcttggct ccgggtagaa gctctcccag aagttggcca ccaggtcgcg
     2461 gcagagggtg aggccgccgg tcagcccggg gaagccgccg gtgcggaaga gccccagcgt
     2521 gagatatccc gaaaccgtca ggtccttcga cttggtcgag aggatggcgt gacccagcgt
     2581 gaccacgttg ttgaagtccg gaatggcccc cgagaccgaa tcgattttct cgatctcggc
     2641 cttgatggcc tcgaattccg gctcgtagac aacggattcc ccccccggac gatcggggga
     2701 aatgggggtc gtgccgaggg gcgccagttc cgcggatgaa aatggcataa gtgacagagt
     2761 agtggattgt tacgagagaa gtcaatcgac gtttcagtgg gttggtggtg gggcgcgggc
     2821 gcgggcaggc cgcgcgcggg tccgggaggt cggccccggg gtcgggccgg cgttggtacc
     2881 ggtgtcgggt cggt
//
LOCUS       DASVOD010000432         3144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_290210, whole genome shotgun sequence.
ACCESSION   DASVOD010000432 DASVOD010000000
VERSION     DASVOD010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290210"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="VF720_09905"
     CDS             <1..1578
                     /locus_tag="VF720_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7879713.1"
                     /translation="STDFGILDRLKKGEEIPLSEFTKGEDDITRQIIEYGGLFPFNVA
                     RLQGKVRVAPIDTPPRSMTLAEKIFARHFVADAAKGVVGVPAVKPGDAGFAAVDLRFS
                     HEYVTPMAADFFHRYVGDDARVNDPSTIVFFRDHLTFLGDVMTPERIKEGLLDVALQL
                     KKKQEDFAAEQGITLHGELAGGHGSEAVCHSKVLQDYALPGQLIIGSDSHTPHSGAIG
                     CVAFGVGTTDVFNSWITKDVRVCVPETVKVRVYGKRPDNVTAKDFMLEILRHPFVKSG
                     KAIGKIMEYCGTAVEELSIDERATMTNMAAEVGAFTGLVAPDQKAVDFLVATRGMNRK
                     AAEATLDGLYSDPDAEYAQIIEIDAGAMKPLVALPGDPGNGLHIDELPGKVKVDIAYA
                     GSCTAGKKDDMDMYHRVLKDGLDHGRKVHPDVRFFIQFGSQEVKQYCEQKGYMATFQA
                     VGAQVIEPSCGACINAGPGVSKTVSEVTVSAINRNFPGRSGPGQMYLASPLTVAASAL
                     AGYITEYRPGVVAGAVS"
     gene            complement(1535..2803)
                     /locus_tag="VF720_09910"
     CDS             complement(1535..2803)
                     /locus_tag="VF720_09910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7879714.1"
                     /translation="MPRPRLLYLATRFPWPLDRGDRIRAYRLIEQFSSLAEVTLVTFH
                     DRPAIEAPVDRILPFCERVEVVHLPRETGVANMLAAVPGRLPFQVAHYRSPAMETLVD
                     RLAREPWDLAFAQLFRMRPYLDRIRARRKVLELSDSLALNLERAAAIKPWWAQVPFRV
                     EWRRVERYERESLSRADESWVVAEPDKADLLARSPGARIEVIPMGLEDRWGGAGLTVE
                     KDRHVLFLGNLTVGHNLDTARYLVGVIWPRVRAVIPDAQLTLVGSAGPRATRLADPVS
                     GVAVTGFVDDLSPLLSRAMFGVAPLRYGAGLQNKVIELMSAGLPSVVTPMVADGIGED
                     ARAALVVAPAPEQFADAMVRLLRDPERARAMGIAGREFVRTRYSWTPARERMAELLRE
                     TESVQRDLRDLRSYPDPSSPPPPPPPADTP"
     gene            complement(2836..>3144)
                     /locus_tag="VF720_09915"
     CDS             complement(2836..>3144)
                     /locus_tag="VF720_09915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7879715.1"
                     /translation="EVGSGIRVRILEAMAWSLPVVSTTIGCEGIEVENGRHLLVADGH
                     EQMANAINRLIKEPVVGQTLRREGRRLVEKKYSLEAVESITGRLYRRLAADPVDSAAG
                     "
BASE COUNT          569 a         1069 c         1028 g          478 t
ORIGIN      
        1 tccacggact tcggcatcct cgatcgcctg aagaaggggg aggagattcc cctctcggag
       61 ttcacgaagg gggaggacga catcacgcgc cagatcatcg agtacggcgg cctcttcccg
      121 ttcaacgtgg cccgcctcca ggggaaggtg cgggtggcgc cgatcgacac gcccccgcga
      181 tccatgacgc tcgccgagaa gatcttcgcc cgtcacttcg tggccgacgc ggcgaagggc
      241 gtggtgggcg tgccggccgt gaaaccggga gacgcgggat tcgccgccgt cgacctccgc
      301 ttcagccacg agtacgtgac gccgatggcg gccgacttct tccaccgcta cgtgggagac
      361 gacgcccggg tgaacgaccc ttccacgatc gtcttcttcc gcgatcacct caccttcctg
      421 ggggacgtga tgacgcccga gcggatcaag gaaggcctcc tcgacgtggc cctccagctc
      481 aagaagaagc aggaggattt tgccgccgag caggggatca cgctccacgg cgaactggcg
      541 ggcgggcacg ggtccgaggc cgtctgccac tcgaaggtgc tccaggacta cgcgctcccc
      601 ggccagctca tcatcggttc cgacagccac acgccccact cgggcgccat cggctgcgtg
      661 gccttcggcg tcggcaccac cgacgtcttc aattcgtgga tcaccaagga cgtgcgcgtc
      721 tgcgtgcccg agacagtcaa ggtgcgggtg tacggcaagc gccccgacaa cgtcaccgcc
      781 aaggatttca tgctcgagat cctgcgccac ccgttcgtga agagcggcaa ggccatcggc
      841 aagatcatgg agtactgcgg caccgcggtg gaggaactct ccatcgacga gcgcgccacc
      901 atgaccaaca tggccgccga agtgggcgcc ttcaccggcc tcgtggcccc cgaccagaag
      961 gccgtcgatt tcctcgtggc cacgcgcggc atgaaccgaa aggcggccga ggccacgctc
     1021 gacggcctct actccgaccc cgacgccgag tacgcccaga tcatcgagat cgacgccggc
     1081 gccatgaagc cgctggtggc cctccccggc gatcccggca acggcctgca catcgacgaa
     1141 ctcccgggca aggtgaaggt ggacatcgcc tacgccggct cctgcaccgc cggcaagaag
     1201 gacgacatgg acatgtacca ccgcgtcctc aaggacggcc tcgaccacgg ccggaaggtg
     1261 caccccgacg tccgcttctt catccagttc ggcagccagg aagtgaagca gtactgcgaa
     1321 cagaaggggt acatggccac gttccaggcg gttggggcgc aggtcatcga gccgtcgtgc
     1381 ggcgcgtgca tcaatgccgg gcccggcgtg agcaagacgg tcagcgaagt gaccgtatcc
     1441 gccatcaacc gcaatttccc cgggcgatcg ggtccggggc agatgtattt ggcgagcccg
     1501 ctgacggtgg cggcttcggc gctggcggga tacatcacgg agtatcggcc gggggtggtg
     1561 gcgggggcgg tgagctaggg tcggggtacg accttaagtc acgcagatcc cgctgcacac
     1621 tctccgtttc ccgcagcaat tcagccatcc tctcccgcgc tggtgtccag ctgtagcgcg
     1681 tgcgcacgaa ttcgcgtccc gcgatcccca tcgcgcgtgc acgttccgga tcgcggagta
     1741 gacgtaccat tgcgtcggcg aactgttctg gcgcgggagc gaccacgagc gccgctctcg
     1801 cgtcttcgcc gatcccgtcg gccaccatcg gtgtgacgac cgaagggagc cccgccgaca
     1861 tcagttcgat gaccttgttc tggaggccgg cgccataccg aagaggcgcg acaccgaaca
     1921 tcgcccgcga gaggagcggc gaaagatcgt ccacaaatcc ggtgacggcg acgccactca
     1981 cggggtccgc caggcgcgtg gcgcgagggc cggcgctccc caccagggtc agttgggcat
     2041 cgggaatgac ggcccgaacg cggggccaga tcacccccac caggtacctg gcggtatcga
     2101 ggttgtgacc aaccgtgaga ttgccgagga agagcacgtg gcgatctttc tcgacagtaa
     2161 gcccggcccc tccccaacga tcctcgagtc ccatggggat gacctcgatc cgcgcgccgg
     2221 gagatcgggc caggaggtcg gccttgtccg gttcggccac cacccacgat tcgtcggccc
     2281 gcgagagcga ctcacgctcg taccgctcga cgcgtcgcca ttccacgcgg aacggcacct
     2341 gtgcccacca tggcttgatg gccgcggccc gctcgaggtt cagggccaac gaatcggaaa
     2401 gctcgaggac cttgcgtcgt gcgcggatac gatcgagata agggcgcatc cgaaagagct
     2461 gggcaaaggc gaggtcccac ggttcccggg ccaggcgatc caccagcgtc tccatggcag
     2521 gcgaccggta gtgcgccacc tggaaaggca ggcggccggg taccgcggcc agcatgtttg
     2581 caacaccagt ctcgcgaggg agatgcacca cctccacgcg ttcgcagaag gggaggatcc
     2641 ggtccaccgg cgcctcgatc gcgggtcggt catgaaacgt caccagcgtg acttcggcca
     2701 ggctcgaaaa ctgctcgatc aggcgatagg cccggatgcg atcgccccga tcgaggggcc
     2761 agggaaaccg ggtagcaagg tagaggaggc gggggcgcgg catccggcgg attgtcgcag
     2821 atgcccggtg cggcgtcagc cggcggcgct gtccaccggg tcggccgcca gccggcgata
     2881 gagccggccc gtgatcgatt ccacggcctc gagcgaatac ttcttctcga cgagcctccg
     2941 tccctcgcgc cggagagtct gccccacgac cggctccttg atcagccgat tgatggcgtt
     3001 ggccatctgt tcgtggccgt cggcaaccag caggtgacgg ccattctcga cttcaatccc
     3061 ctcgcagccg atcgtcgtcg agacgaccgg caggctccaa gccatcgctt cgaggatcct
     3121 cacgcggatg ccgctgccca cctc
//
LOCUS       DASVOD010000433         4285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_393314, whole genome shotgun sequence.
ACCESSION   DASVOD010000433 DASVOD010000000
VERSION     DASVOD010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393314"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF720_09920"
     CDS             <1..279
                     /locus_tag="VF720_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879716.1"
                     /translation="TRPTPNPVTSSATSIRYIVPSRLDSAPVSITIFDVSGRAVRSFA
                     PGTPGAGQHSIDWDLRDADGSEVTNGIYFYRLTVGTESINEKLLVVRQ"
     gene            406..1851
                     /locus_tag="VF720_09925"
     CDS             406..1851
                     /locus_tag="VF720_09925"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879717.1"
                     /translation="MPSLIPRNPIRGRATFRVSCLAISLALLMFATPALAWKFTHSGV
                     DSSGGQPGQFLSMTKGRNGRLHASYFRYTSDDGGKNLAYVTKPSSTTGSWTYKTVDGG
                     SQVGTYSSISLEKNGNPHIAYYESEFANLKHAWYDSSLASWRTEVVDNIGTVGQFTSI
                     AMDTLSGAVNILYFDATQPGLKWARKPVGGLWSFSFVDTNNVCGLYVSAIFTGILEVA
                     YYDLQFGSLRYARQSGNSWVIETVDSPGDVGQFASMAAIGGRRGIAYYDATNHQLKYA
                     ENTGPNMWTKVPIDNVSNPGQGISLAVGPARRPAIAYYAAGTMDLKYAERFRGTWVFD
                     VIATGGDVGRFNGMVMDDVDLPVVLYYDATPGKLRAARGRGEATGVPDVPIEVAPGFL
                     PVLTVRTNPFRDICPLTLTLATPATVGVEVFDVSGRRLRRLDEANYSAGAHELRWDGT
                     DDAGQVVANGLYFLRVLSPAGPVTTRVTRLR"
     gene            1848..2564
                     /locus_tag="VF720_09930"
     CDS             1848..2564
                     /locus_tag="VF720_09930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEX7879718.1"
                     /translation="MRTLGRRLGIFYRQPLPGRVKTRLVPVLTPTEAAWLYNAMLTDT
                     VQMARRLLIDQVVLFEDAERPGWRPPCPDDWQHRAQRGADLGRRMVTAIQELMEGTGP
                     TRACLIGTDAPLLGAPLLETAWDALDAGVDLVTSPTPDGGYAILGMARRPPADFLDGI
                     AWSTGEVLQRTEERARGVGWQAGRVAESRDVDTPDDLSELFTILNSGTLPRKLASAST
                     TRSCLKRLEAAGRWPPATAK"
     gene            2730..>4285
                     /gene="pcnB"
                     /locus_tag="VF720_09935"
     CDS             2730..>4285
                     /gene="pcnB"
                     /locus_tag="VF720_09935"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX7879719.1"
                     /translation="MEPVIVSRSEHPISRKSIDPDAVKVLYRLHRAGFQGYLVGGGVR
                     DLMLGRNPKDFDISTDAHPQQVKRLFRNCRLIGRRFRLAHVHFGGKIVEVSTFRKRSE
                     PVPDDALAEGEEEDILIRSDNTFGTAEEDALRRDFTINGLFYDIGSFAVIDYVAGLDD
                     LKARTIRTIGDPDIRFREDPVRMIRAIKFAARLDFDIEPATWQAILDNHDEILKSAPP
                     RVLEEFYRLLGGGAAARSFHLLEESGMLDILVPQLTEYLAVPERAEASGDGAELPPSP
                     MHRWLTVLDRLRHEDAPLTNALLIGALVGPMAFDDALTSRGPVRSDAARDSGADGSDD
                     GDDDADFYLSPDTEDKIRDVLATIGVPRRDTERLFQTFRAMRRFSGQRSRRFSPRSFV
                     TKNYFPEAFLFLHLAIEATGRPEDVLIRWRKLAEEAGLDGSIPTGSRRRRRRRRRRGD
                     DGRAGFDEPASGDGTGDDFDDAILDEEAVDAASARNGAGDESAPRAAAAPVMRQGRFR
                     REAAPEPVSVP"
BASE COUNT          722 a         1505 c         1374 g          684 t
ORIGIN      
        1 acccgcccga cgccgaaccc ggtgaccagc tccgccacgt ccatccggta cattgttccc
       61 tcccggctcg actcggctcc cgtctcgatc acgatcttcg acgtgagcgg gcgggcggtg
      121 cgctccttcg ctccgggaac cccgggcgcc ggacagcact cgatcgactg ggacctgcgc
      181 gacgcggacg gcagcgaagt caccaacggg atctacttct accggctgac cgtcggcacg
      241 gagtcgatca acgagaagct gctggtcgtc cggcagtagc aacaccgccg gttcacgccg
      301 gcgacacttt catgggctag aatgaaaggg gtcgacccga aggggtcggc ccctttccat
      361 ttccagaaac ccatccgcaa cctgaccgac aaccggtgga gccccatgcc cagcctgatt
      421 cccaggaacc cgataagagg tcgcgcgaca ttccgcgtgt cgtgcctcgc catttcgctg
      481 gccctgctca tgttcgccac gcccgccctc gcctggaaat tcacccactc gggtgtcgac
      541 tcgtccggcg gccagcccgg ccagttcctc tcgatgacca aggggcggaa cggccgtctc
      601 catgccagct acttccgcta caccagcgac gacggcggca agaacctggc gtacgtcacc
      661 aagccctcgt ccaccaccgg gagctggacg tacaagacgg tggacggcgg ctcgcaggtg
      721 ggcacctact cctcgatctc gctggagaag aacggcaacc cgcacatcgc ctactacgag
      781 tcggagttcg ccaacctgaa gcatgcctgg tacgactcgt cgctggcgtc gtggcgcacc
      841 gaggtggtgg acaacatcgg gaccgtcggg caattcacct ccatcgccat ggatacgctc
      901 tccggtgccg tcaacatcct ctacttcgat gcgacgcagc cgggcctcaa gtgggccagg
      961 aaaccggtgg gtggcctctg gagcttcagc ttcgtcgaca cgaacaacgt gtgcggcctc
     1021 tacgtctccg cgatcttcac cggcatcctc gaagtggcgt actacgacct gcagttcggc
     1081 tccctccgct acgcccgcca gagcggcaac agctgggtga tcgagacggt ggacagcccc
     1141 ggcgatgtcg ggcagttcgc ctccatggcg gccatcggcg gccggcgcgg cattgcctac
     1201 tacgacgcta ccaaccatca gctgaagtac gccgagaaca ccggcccgaa catgtggacc
     1261 aaggtcccga tcgacaatgt gtcgaatccg ggacagggga tctcgcttgc ggtggggccg
     1321 gcacggcggc cggcgatcgc gtactacgcg gccggcacga tggacctgaa gtatgccgag
     1381 cgattccggg gcacctgggt gttcgacgtc atcgccaccg gcggggacgt cggtcgcttc
     1441 aacggcatgg tgatggacga tgtcgacctg ccggtcgtcc tctactacga cgccactccg
     1501 gggaagctgc gcgccgcgcg cggcaggggg gaagccaccg gtgtccccga cgtgccgatc
     1561 gaagtggccc cgggctttct cccggtgctc accgtccgga cgaacccgtt ccgtgacatc
     1621 tgcccgctca cgctgaccct ggccacgccg gccacggtcg gggtggaagt gttcgatgtc
     1681 tcggggcgca ggctccgccg gctggacgag gccaactact ccgccggagc ccacgaactc
     1741 cgctgggatg gcaccgacga tgccgggcag gtcgtggcca acggcctcta ctttctgcgg
     1801 gttctctcgc cagcggggcc ggtcacgaca cgggtgactc gccttcggtg agaaccctcg
     1861 gccgccggct cgggatcttc taccggcaac ccctccccgg ccgggtgaag accaggctcg
     1921 ttccggtgct cacgcccacc gaagcggcct ggctctacaa cgcgatgctc accgacaccg
     1981 tgcagatggc ccggaggctc ctcatcgacc aggttgtcct cttcgaggac gcggagcgac
     2041 ccggttggcg gccgccctgc cccgatgact ggcagcaccg ggcccagcgc ggcgcggacc
     2101 ttggcaggag gatggtgacc gcgatccagg aattgatgga gggaaccggc ccgacccgtg
     2161 cctgcctcat cggaacggac gcaccgctcc tcggcgcccc cctcctcgag accgcctggg
     2221 acgcgctcga cgccggggtg gacctcgtca cgagcccgac gccggacggc gggtacgcga
     2281 tcctcgggat ggcccggagg ccgcccgcgg acttcctcga cgggatcgcc tggagcaccg
     2341 gcgaagtcct ccagcgcacg gaagagaggg cacggggagt cggctggcag gccgggcggg
     2401 ttgccgagag ccgtgatgtc gacacccccg atgacttgtc ggaattgttt acgatcctta
     2461 actcagggac gctcccccgg aaactggcct cggcatccac cacgcgctcc tgcctgaaac
     2521 ggctcgaggc ggcgggacgc tggccccctg caaccgcaaa atgaggcaca cacggtcatt
     2581 gaagccgcca acagctttga gtatcattat ttagtcaaac tgccccgccg ccggggccgt
     2641 cccgcggtcg agagtgccgc ggtcgaatga cccgtgacga cacggtcgcg ctcaggcgcc
     2701 ttctccgaaa ttcggaccat cggaccctga tggaaccagt catcgtttcg cgatcggagc
     2761 atccgatctc gcggaagagc atcgatcccg atgccgtcaa agtcctgtat cgactccacc
     2821 gcgccggttt ccagggctac ctggtcggcg gcggcgtccg cgatctcatg ctcggccgga
     2881 atcccaagga cttcgacatc tcgacggacg cccatcccca gcaggtgaag cggctcttcc
     2941 ggaactgccg gctgatcggc cgccgattcc gtctggctca cgtccacttc ggcggcaaga
     3001 tcgtcgaggt gtcgacgttc cgcaaacgga gcgagccggt gccggacgac gccctggccg
     3061 agggagagga agaagacatc ctcatccgga gcgacaacac cttcggcacc gccgaggagg
     3121 atgcgctccg gcgcgacttc acgatcaacg gcctctttta cgacatcggg tcgttcgccg
     3181 tgatcgatta cgtggccggc ctggacgacc tcaaggcccg gaccatccgg accatcggcg
     3241 atccggacat ccggttccgc gaggacccgg tgcgcatgat ccgggccatc aagtttgccg
     3301 ctcgactcga tttcgacatc gagcccgcca cctggcaggc catcctcgac aatcacgacg
     3361 aaatattgaa gagcgccccc ccccgcgtgc tcgaggagtt ctaccgcctc ctgggcggcg
     3421 gcgcggcggc ccgctcgttc cacctgctcg aggagagtgg gatgctcgac atcctggtcc
     3481 ctcagctgac cgagtacctc gcggtgcccg aacgggcgga ggccagtggc gatggggccg
     3541 agctcccgcc gtcacccatg caccgctggc tgaccgtcct cgatcggctt cgccacgagg
     3601 atgcgcccct caccaacgcc ctcctgatcg gggcgctggt cgggccgatg gccttcgacg
     3661 acgcgttgac ctcccggggc cctgtccgga gtgacgcggc ccgcgattcc ggcgccgacg
     3721 gttccgacga cggggatgac gacgccgact tctatctttc gccggacacc gaggacaaga
     3781 tccgcgatgt cctggccacg atcggcgtgc cgcgccgcga caccgaacgg ctcttccaga
     3841 ccttccgggc catgcggcga ttctccggcc agcgaagccg gcgattttca ccgcgatcgt
     3901 tcgtcaccaa gaactatttc ccagaggcgt tcctctttct ccacctggcc atcgaggcca
     3961 cgggccgacc cgaggacgtc ttgattcgct ggcgcaagct ggccgaggag gcgggattgg
     4021 acgggagcat tccgacggga tcacgccggc gtcgtcgccg gcgccggcgc cgcggagacg
     4081 acggacgggc cggattcgat gagccggcgt cgggagatgg aacgggagat gacttcgatg
     4141 atgccatcct ggacgaggaa gccgtcgacg ccgcgtctgc ccggaacggc gcgggtgacg
     4201 agtccgctcc gcgcgcggcc gcggctccgg tgatgcggca gggtcggttc cgccgcgaag
     4261 cggcgccgga gccggtgtcg gttcc
//
LOCUS       DASVOD010000434         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1022375, whole genome shotgun sequence.
ACCESSION   DASVOD010000434 DASVOD010000000
VERSION     DASVOD010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1022375"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VF720_09940"
     CDS             <1..647
                     /locus_tag="VF720_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879720.1"
                     /translation="IAALLAIHAARPDWRHELAWRSWPRQAWHALAVLALALALGVTV
                     DRLELFGRIARPDLWPGPQPPALPGYDAVVPFYDLVWTPFLGAVIAGGVLAGLSLVFR
                     NMLHGTPARVAVGLLAAFALGPDHVNNVGEAVVPTLFEILALGVALLVITRASRGNLP
                     AYLAILPLAMGGQAALVVATQPGPGMAREGWLALAVLALPAMLLVIAGLRNRRT"
     gene            741..>2264
                     /locus_tag="VF720_09945"
     CDS             741..>2264
                     /locus_tag="VF720_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX7879721.1"
                     /translation="MPIDTIGVFRRATRALLVLALGFASPVRAADPATSDTRHQAGPA
                     MPGATVLPRLAAQPAVSLAEVELSDPAAGARLAELGLDVVEVHAERFARVFLWPGDEA
                     VLENSGLVFRVVDRDYARTITEAAERGGTISSDDTGRTPSRDYLAPSAVPAPGSGSFG
                     GWYTLAEVNAFLDSLVTTYPGLCDTMRIGTSRQGRPIRALRITDEGQPNHSRPRVLYT
                     SLTHAREPGGMQALVDFMTRLIQGYGSDPDLTYLVNARELWFVPVVNPDGYEYNRTTW
                     VNSGSFGLWRKNLRDNNGDFAITSADGVDINRNFGYRWGYDNIGSSGIASNQTYRGPS
                     AFSEPETRALRDFSIAHGFTTADNYHTAAELCLYPWGYIVPACPDSTFLTRMSEEMQT
                     ETGYTYGTGGDVLYTVNGDANDWMYGEQVAKPKVFSVTIEAGDDNDGFWPPASRIVPL
                     ARQQFRANVVMAYSAGTWVHADTATIVTGDGWWHPGNWADVAVRLRNDGVAASSGAVS
                     "
BASE COUNT          337 a          835 c          718 g          374 t
ORIGIN      
        1 tcatcgccgc gctgctggcc attcacgcgg cgcggcccga ctggcgccat gaactggcct
       61 ggcgttcctg gccacggcag gcctggcacg cgctggccgt gttggcgctg gccctcgcgc
      121 tcggcgtgac cgtcgatcga ctcgagctgt tcggccgcat cgcccgaccg gatctctggc
      181 caggaccgca gcccccggcc cttcccggct acgatgccgt cgtgccgttc tacgacctcg
      241 tctggacacc gttcctcggc gcggtgatcg ccggcggcgt cctggccggc ctctcgctgg
      301 tcttccgcaa tatgctccac ggaacgcccg cccgcgtggc ggtcgggctc ctcgcagcct
      361 tcgcgctcgg ccccgatcac gtgaacaacg tgggcgaagc ggtggtgccg accctcttcg
      421 agatcctggc actgggcgtg gccctcctcg tgatcacgcg cgcctcgcgc ggcaacctcc
      481 ccgcctacct tgccatcctc ccgttggcga tgggtggaca ggccgccctg gtcgtggcca
      541 cgcagcccgg gccgggaatg gcgcgtgagg gatggctggc gctggccgtg ctggcgctcc
      601 cggccatgct cctcgtgatc gccggacttc gaaaccgccg gacctgatat tctccgggtg
      661 tcgcgctgcg cgaccacccg gatggccttc ccaccgcgca tcctccacac cacatccact
      721 cgacgaggga acgccgaacc atgcccatcg ataccatcgg agtattccgc cgggccaccc
      781 gcgcgctgct ggttctcgcc ctcggattcg cgtcaccggt ccgggcggcg gatccggcca
      841 cgagtgacac gcggcaccag gcaggaccgg cgatgccggg agccacggtg ctgcccaggc
      901 ttgccgccca gccggctgtc tcgttggccg aagtcgaact ctccgatccg gctgccggcg
      961 cgcgactggc cgaactcggc ctggacgtcg tcgaagtgca cgccgaacgg ttcgcccgtg
     1021 tcttcctctg gccaggcgat gaagccgtgc tcgaaaattc cggccttgtc ttccgcgtgg
     1081 tggatcggga ctacgcgcgc acgatcaccg aggcggcgga acggggcgga accatttcga
     1141 gtgacgacac cgggcgcacc ccctcgcggg actaccttgc cccctccgcc gtgccggctc
     1201 cgggttcggg atccttcggg gggtggtaca ccctggccga ggtgaacgcg ttcctcgaca
     1261 gcctcgtgac gacgtatccc ggcctgtgcg acaccatgcg catcggcacg agccgccagg
     1321 gtcgccccat ccgggcgctc cgcatcaccg acgaggggca gccgaaccac tcgaggccgc
     1381 gcgtgctcta tacctcgctc acccacgccc gcgagccggg cggcatgcag gcgctggtcg
     1441 acttcatgac acgcctcatc cagggttacg gcagtgatcc cgacctgacc tacctcgtca
     1501 acgcgcgcga actctggttc gtgcccgtgg tgaatcccga cggctacgaa tacaaccgca
     1561 ccacatgggt gaacagcggc tcgttcggcc tgtggcgcaa gaacctgcgc gacaacaacg
     1621 gcgactttgc catcaccagc gccgacggcg tcgacatcaa ccgcaatttc ggttaccgat
     1681 ggggttacga caacatcggt tcgtccggga tcgcgtcgaa ccagacctat cgcggccctt
     1741 ccgctttcag tgagccggaa acgcgggcgc tgcgcgactt ttcgatcgcg cacggcttca
     1801 cgacggcgga caactaccac accgccgcgg aactctgcct ctacccgtgg ggctacatcg
     1861 ttccggcctg ccccgattcc accttcctga cccgcatgtc cgaggagatg cagacggaga
     1921 ccggctacac ctacggaacc ggcggggatg tcctctacac ggtgaacggc gatgcgaacg
     1981 actggatgta cggcgagcag gtcgccaagc cgaaggtctt ctcggtcacc atcgaggccg
     2041 gcgacgacaa cgatggattc tggccccccg cctcccgcat cgtgccgctg gcccgccagc
     2101 agttccgggc caacgtggtg atggcctact cggccggcac ctgggtccat gccgacaccg
     2161 ccacgatcgt gaccggcgac ggctggtggc accccggcaa ctgggccgat gtcgccgtga
     2221 ggctccgcaa tgacggtgtg gccgcttcga gcggcgcggt ttcg
//
LOCUS       DASVOD010000435         3109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_549947, whole genome shotgun sequence.
ACCESSION   DASVOD010000435 DASVOD010000000
VERSION     DASVOD010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549947"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF720_09950"
     CDS             complement(<1..252)
                     /locus_tag="VF720_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879722.1"
                     /translation="MNPARPTRAPHSPLDLGHASTDGILATLEELGLSTDREAFRTLA
                     RRRGSPSLVADTWLESIGMRVEPTGPEPPRPVDADPSATN"
     gene            complement(323..1039)
                     /locus_tag="VF720_09955"
     CDS             complement(323..1039)
                     /locus_tag="VF720_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX7879723.1"
                     /translation="MAPSSRRPRGDLSDLNARIVACTACPRLIAHCREVARLKRRAWA
                     HETYWGRPVPSFGDPSARLLVVGLAPAAHGGNRTGRVFTGDSSGDWLYDALHRAGFAN
                     QPTSTGLEDGLVLTDAWVTATNHCAPPDNKPSLEEQAACRPFLVEEIGRLARVRVVLA
                     LGRIAWDGWFRAMADAGHPIPRPRPGFGHGVEVRPFVNEGPLLLGSFHPSRQNTNTGK
                     LTRAMWHAVFARARVLVESD"
     gene            1281..1988
                     /locus_tag="VF720_09960"
     CDS             1281..1988
                     /locus_tag="VF720_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879724.1"
                     /translation="MVYGVDALVAHGVPFVASHGGDIILRLDPDSRDEALTLPGATEW
                     NPMGRGEADFVIVPGAYVAADDVLRYWVERAYEYAESLASIDNDEVHFDDDMDSFGHD
                     DMSALSEMSPAPRPKPTPKPAAKPAAKAEAPKASAPAPAPKPAAKPAAKKSAPVKAAA
                     KKAVVAKAVKKAVAKKVAKKAVVKKAIKKAAAKKVAKKAVRRAVVKKTVAKKVAKKAV
                     KRAVVKKAVVKKAARKK"
     gene            complement(2141..3010)
                     /locus_tag="VF720_09965"
     CDS             complement(2141..3010)
                     /locus_tag="VF720_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879725.1"
                     /translation="MRSRSMKWLFPLALVAIISAGCSEDDILDPVDPEDLAPPLGLTS
                     ITGDGQVVLSWQASNYDEDRDGFYVFQANGAQTTNTPEDIPSAFGTSPVATLTTTQDA
                     GEFTQTVTGLANGTTYSFLVVAFKNDGDDVSRPSNIIVDTPREESDGVLALTNGSGNR
                     FLDVDTQTVQANDDTPNSADVLSQSFNAGAGDRHGMVGVNGARIQDLGYVSSWDEVDD
                     VPTGTASYPATNYSVQVLVGHVYAVYTGDNHYAKVYVTSLNSGNFGYSVRVAYQPQAG
                     NNELNPGGPVGGN"
BASE COUNT          531 a          950 c         1121 g          507 t
ORIGIN      
        1 gttcgtggcg gacggatctg cgtccacggg ccgcggcggc tcgggaccgg tcggttcgac
       61 cctcatcccg atcgactcga gccaggtgtc cgctaccagc gacgggctcc cgcggcggcg
      121 ggccagtgtg cggaaagcct cccggtcggt cgagaggccc agttcctcga gcgtggccag
      181 gatgccgtcg gtggatgcgt gtcccaggtc gagcggggag tgcggggcgc gggtggggcg
      241 tgccgggttc atgggtcgat ctcccggacg gggtgatcgg ggtgggtcga aaacgatcgt
      301 ggacaggatg cggccgagct gtctagtccg attcgacgag tacccgcgcc cgggcgaaca
      361 ccgcgtgcca catcgcccgg gtgagcttgc cggtattcgt gttctggcga ctgggatgga
      421 atgagccgag caggagcggc ccctcgttca cgaaggggcg tacctcaact ccatgcccga
      481 agccggggcg gggacgcggt atggggtgcc cggcgtcggc catcgcccgg aaccagccgt
      541 cccaggcgat ccggccgagg gccagcacca cccgcacccg ggccagtcgg ccgatctctt
      601 ccaccaggaa tgggcggcag gcggcctgtt cctcgagcga gggcttgttg tccggcgggg
      661 cgcagtgatt ggtggccgtt acccaggcgt cggtgaggac cagcccgtcc tcgagccccg
      721 tggaggtggg ctggttggcg aagccggccc ggtggagggc gtcatacagc cagtcgccgg
      781 acgaatcgcc cgtgaaaacc cggcccgtac ggttgccccc gtgtgcggcg ggggccaggc
      841 ccaccaccag gagccgggcg gacgggtctc cgaacgaggg taccgggcgt ccccagtacg
      901 tttcgtgggc ccaggcccgc cgtttcaaac gggccacctc gcggcaatgg gcgatgagcc
      961 gggggcaggc cgtgcaggcc acgatccggg cgttcaggtc cgagagatca cccctgggac
     1021 ggcgggacga gggagccatg gggcgtcgag aatgtcgggg gcggggcgca tcgtcaatca
     1081 tccatcttga cggaattgac caatgccatg aggatggggc cgagggcgcc ggcgcgtcgc
     1141 ggaccggacc cggaacggct gaatcgcggg acgatcggcc gtacgaccca cgtggcgccc
     1201 cggggggcgc ccccaacgag gtgaccatgc tggaacggat tcgaatgtgc ctgccgtcag
     1261 gcgccgaggt agacctccgg atggtatacg gcgtcgacgc cctcgtcgcg catggcgtgc
     1321 ctttcgtggc atcgcacggc ggcgacatca tccttcgcct cgatcccgat tcccgcgatg
     1381 aagccctgac tttaccgggc gccaccgagt ggaacccgat gggtcgcggc gaggccgatt
     1441 tcgtcatcgt tccgggcgcc tacgtggccg cggacgatgt cctccgctac tgggtggagc
     1501 gcgcctacga atacgccgag agcctggcct ccatcgataa cgacgaagtc cacttcgacg
     1561 acgacatgga ttccttcggc cacgacgaca tgagcgcact ttccgagatg tccccggccc
     1621 cgcgccccaa acccacgccg aagccggccg ccaagccggc cgcgaaggcg gaggcgccga
     1681 aggcctccgc tccggctccc gcgccgaagc cagctgcaaa gcctgcggcg aagaagagcg
     1741 ctccggtgaa ggcggctgcg aagaaggcag ttgtcgccaa ggccgtgaaa aaggccgtgg
     1801 cgaagaaagt tgcgaagaag gcggtcgtga agaaggcgat caagaaggcc gcggcgaaga
     1861 aagtcgcaaa gaaggctgtt cgccgcgcgg tcgtgaagaa gaccgtggcg aagaaggttg
     1921 cgaagaaagc cgtgaagagg gcggtcgtga agaaggccgt cgtgaaaaag gcggccagga
     1981 agaagtaggg acgcttgtcc agcgacgtcg aataacgatg tcgaaggtaa attcagggag
     2041 ataacaatga gggcgggcca tgtggcccgc cccctttttc ttgtcttgtc aggacgacat
     2101 ttgtttcctg catcggtccc gcggaatgga agcaacgcgt ctagttgccg ccgaccggtc
     2161 caccggggtt gagctcgttg ttgccggcct gcggctggta cgccacgcgg acgctgtagc
     2221 cgaagttgcc gctgttgagg ctggttacgt acaccttggc gtagtgattg tcgccggtat
     2281 acacggcata aacatggccg accaggacct gtaccgagta gttcgtggcc ggatacgagg
     2341 ccgtaccggt cgggacatca tcgacctcgt cccagctgct gacgtacccg aggtcctgga
     2401 tgcgggcgcc gttcacgccc accatgccgt ggcggtcacc cgcgccggcg ttgaacgact
     2461 gggagaggac gtcggccgag ttcggcgtgt cgtcgttggc ctggaccgtc tgcgtatcca
     2521 cgtcgaggaa gcggttgccg ctgccgttgg tcagcgccag cacgccatcc gattcctcgc
     2581 gcggcgtatc gacgatgatg ttgctcgggc ggctcacgtc gtcaccgtcg ttcttgaagg
     2641 ccacgacaag gaagctgtag gtggtgccgt tggcaagccc cgtgacggtc tgcgtgaact
     2701 cgccggcgtc ctgcgtcgtg gtgagggtgg ccacgggaga cgtgccgaac gccgacggga
     2761 tgtcctccgg cgtattggtc gtctgtgcgc cgttggcctg gaacacgtag aagccgtcgc
     2821 ggtcctcgtc gtaattgctg gcctgccacg acaggaccac ctggccgtcg ccggtgatgg
     2881 aggtgaggcc gagggggggc gccaggtcct cggggtcgac cggatccaga atgtcatcct
     2941 cactacaccc ggcgctgatg atcgccacca gtgccagcgg aaaaagccac ttcatcgagc
     3001 gtgaacgcat gttggtcctc ctcgttgggt cgtgctcgtg ctgtgaatgg aatgtggatt
     3061 atttccggca gagggccagg tgccgggtat gcggccaggg gacgatcct
//
LOCUS       DASVOD010000436         5783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_341084, whole genome shotgun sequence.
ACCESSION   DASVOD010000436 DASVOD010000000
VERSION     DASVOD010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341084"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="serB"
                     /locus_tag="VF720_09970"
     CDS             <1..1022
                     /gene="serB"
                     /locus_tag="VF720_09970"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEX7879726.1"
                     /translation="LRVVGGTALALGLTVDARPIESAEAGRLGHRDLWALTILSDGLD
                     VATVARLARLVGSHRANIVTIHRLAEDDLSAVDFILDVTRTPDLAELKSDLLHLSDAT
                     GIDLGLQPEDAFRKSKRLVVFDVDSTLVNGEGIDELAREAGKGEEVASLTRAAMDGRL
                     DFGEALRCRVALLEGLSVEAVRRAAARLPLTPGAGQTVEILKELGYRVAAISGGFDLL
                     VDPLRQELKLDHAYANRLELADGRLTGRVLGDIVDAAGKAALLRSIAADERIPLEQVV
                     GVGDGANDIPMLQAAGLGIAFGRRDRIRRAAHGAIARPDLGGILYLLGVTGRDLTALR
                     RRRRA"
     gene            complement(1543..2292)
                     /gene="sdhB"
                     /locus_tag="VF720_09975"
     CDS             complement(1543..2292)
                     /gene="sdhB"
                     /locus_tag="VF720_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010151655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEX7879727.1"
                     /translation="MIARTVHVRVRRQDGPDQPSRWEDFDVPYRQGMNVITCLQDIQK
                     NPKTRDGRTVAPVVWECSCLEEVCGSCTMVINGRPRQSCSALVEQLDQPIRLEPMTKF
                     PLVRDLVVDRSRMFEALKKVKSWVTLDGTHNLGPGPRFSPEKQQVYYHLQRCMTCGCC
                     LEVCPQVTKENDFVGAAAISQVRIVNAHPSGAMHAAERLDALMGEGGIQDCGNAQNCV
                     EACPKEINLVESIADMNRQVTRRLFGKMFGG"
     gene            complement(2315..4156)
                     /gene="sdhA"
                     /locus_tag="VF720_09980"
     CDS             complement(2315..4156)
                     /gene="sdhA"
                     /locus_tag="VF720_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEX7879728.1"
                     /translation="MAEKPRIIVVGGGLAGLMTVIKVAEAGHPVDLFSVVPVKRSHSV
                     CAQGGINGAVNTKGEGDTPWEHFDDSIYGGDFLADQPPVQKMCEAAPGIIYMFDRMGV
                     MFNRTPEGLLDFRRFGGTKHHRTAFAGATTGQQLLYALDEQVRRYEVAGLVTKYEGWE
                     FVGYVPDEAGECRGAICQHMASGRLQSFRSDAVVLATGGPGLIFGRSTNSTINTGSAA
                     SAAYQQGAWYANGEFIQVHPTAIPGEDKLRLMSESIRGEGGRVWVPSDGNEGRPAKGQ
                     PWYFLESMYPAYGNLVPRDIATRAIFKVCRELGLGIDGKDKVYLDVTHIPARVLNAKL
                     EGVMEIYEKFVGQDPRKVPMQIFPAVHYSMGGLWVDYPRDDGKPHYWQMTNVPGLFAV
                     GECEYAYHGANRLGANSLLSTVFAGMMGGPDALRFAKGRRSTAADTPASVFESEMARH
                     EAFNQRLLSLDGPENPFQLHAELGDLMTGAMTVVRYNDKLEAAMGSIAALRERFWNIT
                     LTDKGAWMNQPLLFSRQLHNMLILAEAMTLGALRRDESRGAHYKPDFPERNDERFLKT
                     TLARFTPAGPELSYVDVDTSLIPPRARKYDVEKKGDAAKPAGALATS"
     gene            4578..4781
                     /locus_tag="VF720_09985"
     CDS             4578..4781
                     /locus_tag="VF720_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HEX7879729.1"
                     /translation="MKITYFRDTDMLSIQLRDVPSTESEEVAPGFVFDFGVDGAVVAL
                     EIEHASHHVDIRGVTVQALDVLS"
     gene            complement(4783..4935)
                     /locus_tag="VF720_09990"
     CDS             complement(4783..4935)
                     /locus_tag="VF720_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879730.1"
                     /translation="MGSESLFLTAGAPVWEFVERGGSYAAPLALMWLKARQSLPVPQV
                     WRDQAR"
     gene            complement(4925..>5783)
                     /locus_tag="VF720_09995"
     CDS             complement(4925..>5783)
                     /locus_tag="VF720_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX7879731.1"
                     /translation="MFGTMDVDALRAMIRNDRAAGFLPMMLVGNAGTVSTGAIDPLNT
                     QADIAAAEKMWFHVDGAYGGFAACLPESSPDMKAIARADSVAIDPHKWLYVPIECGCT
                     LVRTREHLVDTFSYRPPYYHRDERDEIPPIYYYEMGPQNTRGFRALKTWLALQQAGRS
                     GYESMLRENCRLATLLFEAAGQHPELETFTVGLSIATFRYVPPSFRDPATPGPMAGTP
                     EGEAALNDLNTRLVERLQAGPDAFVSNAVLNGTYVLRACCVNFRTTEDDMRRLPDIVV
                     RAGRQLHGE"
BASE COUNT          954 a         1840 c         1941 g         1048 t
ORIGIN      
        1 tgttgcgcgt tgtcggtgga accgccctcg cactcggact gacggtcgat gcccggccga
       61 tcgagtcggc cgaagccggc cggctgggcc atcgcgacct ctgggccctc accatcctga
      121 gcgacggact cgacgtggcc accgtggcgc ggctcgcccg tctcgtcgga agtcatcggg
      181 ccaacatcgt cacgatccac cggctcgccg aagacgacct gtcggccgtc gacttcatcc
      241 tcgacgtgac ccgcactccc gacctggccg agctcaagtc ggatctgctc cacctctcgg
      301 atgccaccgg aatcgatctc ggactccagc ccgaggatgc cttccggaag agcaagcggc
      361 tcgtcgtctt cgacgtcgat tcgaccctgg tgaatggtga ggggatcgat gagctggcgc
      421 gggaggcggg gaagggagag gaggtggcat ccctcacacg cgccgccatg gacggccggc
      481 tcgatttcgg ggaggccctt cggtgtcgcg tggcgctcct cgaagggctg tcggtggagg
      541 cggtccggcg ggcggccgcc cggttgccac tcacgccggg cgccggccag accgtcgaga
      601 tcctgaagga gctgggctac cgggtggcgg ccatcagcgg tggcttcgac ctgcttgtcg
      661 atccgctcag gcaggaattg aagttggacc acgcgtacgc caaccggctg gaactggccg
      721 acggtcggct cacggggcgg gttctgggtg acattgtcga cgcggcaggc aaggccgcac
      781 ttctccgttc cattgccgcg gacgagcgca tcccattgga acaggtcgtc ggtgtcggcg
      841 acggggcgaa cgatatcccg atgctccagg cagccgggct gggaatcgcc ttcgggcgtc
      901 gcgacaggat ccgccgagcc gcccatggcg ccatcgcccg acccgacctc gggggaatcc
      961 tttatttact tggggttacc ggccgggacc tgacggccct ccgccggcgg cggcgcgctt
     1021 gacagtttcc gcggcgctct gtaaactctc ctcacctctg gctccatgtg aagcgaagcc
     1081 tcgacctgat tgaagctctt gaggtagggg cagcgggaat ttcaagccag tgttttcgga
     1141 tcaaaaaaag cgtcaattcc ctttgacgct gcgaaccgga agcgcggagc ggggtatcaa
     1201 agccggaaca aggttttcgc gaggttaacc gggaccgcgc cgccctgcgg gttctaggag
     1261 ataccaagcg atgggagacg atcgcaagat cgtctccttt tttttgtgcg aaatcattcc
     1321 tgaaatcgtt tgacacagtg ggtctctttt ttacgcatta cagaatgccg ctgggagaga
     1381 gagcgccagg gtgacgggtt tcgttgtcag ggcaggacac tacgagctcg gacttcaggc
     1441 agggactcct cagccggccg ggtcggggga aactccggcc cggccaactt ttttcctgtc
     1501 gaatcgagaa ttcaacggga gaaggatgcc acgggcgccg cgttagccgc cgaacatctt
     1561 cccgaagagc cgccgcgtca cctgtcggtt catgtcggcg atcgattcca ccaggttgat
     1621 ctccttcggg cacgcctcca cgcaattctg ggcgtttccg cagtcctgga tgccaccttc
     1681 gcccatcagg gcatccagcc tttccgccgc gtgcatcgcc ccggatggat gggcattcac
     1741 gatccgcacc tggctgatgg ccgcggcccc gacgaagtca ttctccttcg tcacctgggg
     1801 gcacacctcc aggcagcatc cgcaggtcat gcaccgctgc aggtggtagt aaacctgctg
     1861 cttttcgggg ctgaaacgcg ggccgggccc caggttgtgg gtgccgtcga gcgtcaccca
     1921 cgacttcacc ttcttcaggg cctcgaacat gcggctgcga tcgaccacca ggtcgcgcac
     1981 cagcgggaac ttggtcatcg gctcgagccg gatcggttgg tcgagctgtt cgaccagggc
     2041 gctgcatgac tgccgcggcc gcccgttgat gaccatggtg caggagccgc acacctcttc
     2101 caggcaggag cactcccaga cgaccggcgc caccgtgcgg ccgtcgcgtg tcttcggatt
     2161 cttctgtatg tcctggaggc aggtgatgac attcattccc tgccggtagg ggacatcgaa
     2221 gtcctcccag cgggagggtt gatccgggcc gtcctggcgc cggacgcgga catgaacggt
     2281 gcgggcgatc atgaggggag gctccctgtc gggttcatga ggtggccagg gcgccggccg
     2341 gcttcgcggc atcgcccttc ttctcgacgt cgtacttgcg ggcccggggc gggatcaggc
     2401 tggtatcgac atccacgtac gagagttccg gcccggccgg cgtgaagcgc gccagcgtgg
     2461 tcttgaggaa gcgctcgtcg ttccgctccg ggaagtccgg cttgtagtgg gcgccccggc
     2521 tttcgtcacg ccggagtgcc cccagcgtca tggcctcggc caggatcagc atgttgtgga
     2581 gctggcgact gaacaggagc ggctggttca tccaggcacc cttgtcggtg agcgtgatgt
     2641 tccagaagcg ctcgcgcagg gccgcaatgg aacccatcgc cgcttcgagc ttgtcgttgt
     2701 agcgcaccac ggtcatggcc ccggtcatca gatcgcccag ttccgcgtgg agctggaacg
     2761 gattttccgg gccgtccagc gacaggagac gctggttaaa cgcttcgtgc cgtgccatct
     2821 cggactcgaa gacggaagcc ggcgtgtccg ccgctgtcga gcggcgcccc ttcgcgaacc
     2881 ggagggcgtc cggccccccc atcatcccgg cgaataccgt ggagaggagg ctgttggcgc
     2941 cgagtcggtt ggcgccgtga taggcgtatt cgcactcccc gacggcaaag aggcccggca
     3001 cgttggtcat ctgccagtag tgcggcttgc cgtcgtcgcg cgggtagtcc acccagaggc
     3061 cacccatcga atagtgcacc gccgggaaga tctgcatcgg caccttgcgc gggtcctggc
     3121 cgacgaattt ctcgtagatc tccatcacgc cttccagctt ggcgttgagc accctggccg
     3181 ggatgtgcgt gacatcgagg tacaccttgt ccttgccgtc gatgccgagg cccagttcgc
     3241 ggcacacctt gaagatggcc cgcgtggcga tgtcgcgcgg gacgaggttg ccgtaggcgg
     3301 gatacatcga ctcgaggaag taccagggct gacctttggc cgggcgcccc tcgtttccgt
     3361 cggacggcac ccacacgcgc cccccttcgc cgcggatcga ctcggacatg aggcgcagct
     3421 tgtcctcgcc cgggatggcg gtggggtgca cctggatgaa ctcgccgttg gcgtaccagg
     3481 cgccctgctg gtaggcggcg ctggccgccg agccggtgtt gatggtggag ttggtggagc
     3541 ggccgaagat gagtccgggc ccgccggtgg ccaggacaac cgcgtcggag cggaatgact
     3601 ggagccgccc cgaggccatg tgctggcaga tcgcgccgcg gcactcgccg gcctcgtccg
     3661 gcacgtagcc cacgaactcc cacccctcgt acttcgtgac gagccccgcc acctcgtaac
     3721 ggcgcacctg ctcgtcgagc gcgtagagga gctgttgtcc cgtggtggcg cccgcgaagg
     3781 cggtgcgatg gtgcttggtt cccccgaacc gacggaagtc gagcagcccc tcgggcgtgc
     3841 gattgaacat gacgcccatg cggtcgaaca tgtagatgat gccgggagcc gcctcgcaca
     3901 tcttctgcac cggcggctgg tcggccagga agtcgccgcc gtagatcgaa tcgtcgaaat
     3961 gctcccaggg ggtatcgccc tcccccttgg tattcacggc cccgttgatg ccgccctggg
     4021 cgcataccga gtgcgagcgc ttgaccggca ccacggaaaa gagatccacg gggtgacccg
     4081 cctcggccac cttgatgacg gtcatgagcc cggcgagccc gccgccgacg acgatgatgc
     4141 gcggtttttc ggccattttc gaaggtccct gcgaatgatt ggatcggacg tctgcgccgc
     4201 tgcgcgacat cgcaccggcc tcgcggccgg acttcggact atacgacgtg ggtgggagtg
     4261 gggaaaggcg gttgacggtt cggagcgaag tcaggttagg gttgagcagg attacccgtc
     4321 ctccgtggag ggtgggtgtc ccgacgattt caggtcacga attacttcat acaccgcgtc
     4381 atgttcgatc gaccgtatgc gaccatcgag ttgtgcatca gggtgattcg gaacccggag
     4441 aaagtggaaa ttcagcccga tgggcgtatc cgcttctggg cacgacttcc cgagttggaa
     4501 ggacgggcgc taagagtcgt gacactgaac gacgctcgca cgatcctgaa cgccttcatg
     4561 gacaggaggt ttcggccatg aaaatcacat actttcgcga caccgacatg ctctcgatcc
     4621 agcttcgcga cgtacccagt accgagagcg aagaggtcgc gccgggtttc gtcttcgact
     4681 tcggagttga cggagccgtg gtggctctcg agatcgagca cgccagtcat catgtggata
     4741 ttcgaggggt gactgttcag gcgcttgatg tgctgagcta gcctaacgcg cctggtcgcg
     4801 ccagacctga ggcacgggca gggactgccg cgccttgagc cacatcaacg ccagcggcgc
     4861 cgcgtaacta cccccgcgct ccacgaactc ccacaccggc gcgccggccg tgaggaagag
     4921 gctttcactc cccatggagt tgccgccccg cccgcaccac gatgtcaggc aggcgccgca
     4981 tgtcgtcctc cgtcgtgcgg aagttcacgc agcacgcgcg aaggacatac gttccgttca
     5041 acacggcgtt cgacacgaag gcgtcgggac cggcctggag ccgctcgacg agtcgcgtgt
     5101 tgaggtcgtt cagcgccgct tccccctcgg gcgtgcccgc catggggcct ggtgtggcgg
     5161 gatcgcggaa cgagggcggc acgtagcgga aggtggcgat cgagagcccc acggtgaacg
     5221 tctcgagctc gggatgctgg ccggcggcct cgaagagcaa ggtggccagg cgacagttct
     5281 cgcgcagcat cgattcgtaa ccggagcgcc ccgcctgctg gagcgccagc caggtcttga
     5341 gcgcgcggaa accgcgggtg ttctgcggcc ccatctcgta gtagtagatc ggcgggatct
     5401 cgtcgcgctc gtcgcggtgg tagtaggggg gccggtagct gaaggtatcc acgaggtgct
     5461 cgcgcgtgcg cacgagcgtg cagccgcact cgatcggcac gtagagccac ttgtgcgggt
     5521 cgatggccac cgaatcggcg cgggcgatgg ccttcatgtc gggcgacgat tccggcaggc
     5581 aggccgcgaa gccgccgtag gcgccgtcca cgtggaacca catcttctcg gcggcggcga
     5641 tgtcggcctg cgtgttcagt ggatcgatcg cgccggtcga gacggtgccg gcatttccca
     5701 cgagcatcat cggcaggaag cccgccgcgc ggtcgtttcg gatcatcgcg cgaagcgcgt
     5761 ccacgtccat cgtgccgaac atc
//
LOCUS       DASVOD010000437         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_550223, whole genome shotgun sequence.
ACCESSION   DASVOD010000437 DASVOD010000000
VERSION     DASVOD010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550223"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF720_10000"
     CDS             <1..294
                     /locus_tag="VF720_10000"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HEX7879732.1"
                     /translation="LWPGYGENTRVLKWVFERVTGGGEAVDTPIGRLPATGAIDTAGL
                     SLSEAAMKELMTVDIHGWTAELPLIKEHYAKFGSKLPSGLSDELAQLEVRLRG"
     gene            complement(291..854)
                     /locus_tag="VF720_10005"
     CDS             complement(291..854)
                     /locus_tag="VF720_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6174 domain-containing protein"
                     /protein_id="HEX7879733.1"
                     /translation="MIAITALVLPLAGCYTQLKTNPRPEVVDSGSEELPPGHPPIPPD
                     QGNLPPGHRPTGPPGPGSANGAPVIGPSYEPNIDRNTARNLWRDQGISTYSYRLRNET
                     QGVREEYTVDVERSNVVNVRDYYGNGYPPHPADALTINHWFDALWNEGGYGASYDGAD
                     GHPLRFEFRRPGSSVGSVHTILWLERY"
     gene            complement(911..1675)
                     /locus_tag="VF720_10010"
     CDS             complement(911..1675)
                     /locus_tag="VF720_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7879734.1"
                     /translation="MRASTPKHWDDYWKKQDDLDDAYSNEDRLIDGLLQLMDPKGKKI
                     LEVGGGSGRDSIRLAQMGADVTVIDYVESSLGVVARNAERAGVHITLVHGDALKMPFP
                     EGTFDVVFHQGLMEHFRDEQPLLLENRRVLKRGGLVLIDVPQKFHIYTVFKQTLIAMN
                     KWFAGWETQYSIDELEAIVRKAGFEVAGRYGDWMCPGFFYRAARRGLAGTGLKLPKYP
                     RVPPFAQINDAWRGFWRRTPVAIYTYAMIGSIGRKP"
BASE COUNT          307 a          587 c          585 g          315 t
ORIGIN      
        1 ctctggcccg gctacggcga gaacacccgc gtgctcaagt gggtattcga acgcgtcacc
       61 ggcggcggcg aagcggtgga tacgcccatc ggccgccttc cggccaccgg cgccatcgac
      121 accgccggcc tgagtctttc cgaggcggcg atgaaggaac tgatgacggt cgacatccac
      181 ggctggaccg ccgagctccc gctcatcaaa gagcactacg ccaagttcgg atcgaagctg
      241 ccgtcgggac tgtcagacga gctggcgcag ctcgaggtgc ggctgagggg ctagtaccgc
      301 tccagccaga ggatggtgtg cacgcttcca acgctcgaac ccgggcggcg gaactcgaag
      361 cggagcggat ggccgtcggc gccgtcgtaa ctggcgccgt acccaccctc gttccagagc
      421 gcgtcgaacc agtggttgat ggtcagtgca tcggccgggt gcggcggata gccgtttccg
      481 taatagtcac gcacgttcac cacgttgctg cgctccacgt ccaccgtgta ttcctcgcgc
      541 accccctgcg tctcgttgcg cagccgatac gagtaggtgg atatcccctg gtcgcgccag
      601 aggtttctcg ccgtgttccg atcgatgttc ggttcgtaac tcggtccgat cacgggcgcg
      661 ccattcgcgc tgcccggtcc aggcgggcca gtcgggcgat gccccggtgg aaggtttccc
      721 tggtcgggcg ggatcggcgg atgtcccggc ggcagttctt cggagccgct atccaccacc
      781 tcggggcggg gattcgtctt gagctgcgtg taacagccgg caaggggcag gacgagcgcc
      841 gtaatcgcaa tcaaggcggg aaatcgggag cgaaacaggg cgtatctccg ttcggccggg
      901 gtggcgtact tcagggcttg cgcccgatcg acccgatcat ggcgtacgtg tagatggcca
      961 ccggtgtccg gcgccagaat ccgcgccagg cgtcgttgat ctgcgcgaac ggcggtaccc
     1021 gtggatattt cgggagtttc agtcccgttc cggccagccc gcgccgcgcc gcgcgataga
     1081 agaagcccgg gcacatccaa tccccgtagc gccccgccac ctcgaagccc gccttgcgga
     1141 cgatggcttc cagttcgtcg atggagtact gcgtctccca gccggcaaac cacttgttca
     1201 tggcgatcag ggtctgcttg aacaccgtgt agatgtgaaa cttctgcggc acgtcgatca
     1261 gcaccagccc gccgcgcttc aacacgcggc gattctcgag gaggagcggc tgctcatcgc
     1321 ggaagtgctc catgagcccc tggtggaaca ccacgtcgaa cgtcccctcg ggaaacggca
     1381 tcttgagcgc gtcgccatgc acgagggtga tgtgcacccc cgcccgctcg gcgttccgcg
     1441 ccacgacccc gagcgacgac tccacgtaat cgatgaccgt gacgtccgcc cccatctgcg
     1501 ccagccggat cgaatcacgc cccgatccgc cgcccacctc gaggatcttc ttccccttcg
     1561 gatccatgag ctggagcaac ccgtcgatga gcctgtcctc gttggaatag gcgtcatcca
     1621 ggtcgtcctg cttcttccag tagtcgtccc agtgcttggg agtggaggcg cgcatgtcgg
     1681 tttccggtca ggagtaaggg cgagggaagc gggaaaggct agcagcgaag gaaagcgcca
     1741 tcaagcgggc gccgcgccga tgtcccgccg aaggcggaag ggtattggag tcgg
//
LOCUS       DASVOD010000438         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_863853, whole genome shotgun sequence.
ACCESSION   DASVOD010000438 DASVOD010000000
VERSION     DASVOD010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863853"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1767
                     /gene="purL"
                     /locus_tag="VF720_10015"
     CDS             <1..>1767
                     /gene="purL"
                     /locus_tag="VF720_10015"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEX7879735.1"
                     /translation="CSYKSSRPVLKEFLPTEAPNVLLGPGEDAGIIYLTSHKGERWGL
                     VVAHESHNHPSQVVPAEGAATGVGGIVRDVYCMGADVVGVLDALRFGDLTGPNGERCR
                     EIAEGVVNGIEQYANALGVPNLGGDAWFDPGYDDNCLVNVVAVGVVREKEIIRSRVPK
                     EAHDEPYDLILLGKPTDSSGFGGASFSSAVLSEDDLESNKGAVQLPDPFLKRVIVEAT
                     RKALAFCRERKAAIGFKDLGAGGIACVTSELAAHGGFGMDVELDRETQEGGPYPGEVV
                     ACSETQERFGLVVPARLSEEILAIYNRDFELPRLYPGAGARVIGRVRTDGQYRVSYRG
                     ETVVDAPVDVITEGIRYDRPARPAPPAARGQALPPIDDMADLILRMVAHPNVASREYI
                     YRHYDTEVQARAVLRPGEADASLFLPIPGSPVGVAVSVDGNPAYGRLDPYRGGLTAVA
                     EAMRNVAAVGAVPQCLTDCLNFGNPEDPEVFHEFRESVRGLGEAARRLGLKGEPGSPV
                     PFVSGNVSLYNFSTAGRAIPPSPIVACFGILEDYSKAVGLTIREPRSLLVLAGERRAE
                     FGGSIAAQVLGVTGGEPPTVDF"
BASE COUNT          263 a          606 c          629 g          269 t
ORIGIN      
        1 ctgctcgtac aagtcgagcc gcccggtcct caaggagttc ctcccgaccg aggcgcccaa
       61 cgtgctgctc gggccgggcg aggatgccgg catcatctac ctcaccagcc acaaggggga
      121 gcgatggggg ctggtggtgg cgcacgagag ccacaatcac cccagccagg tcgtgccggc
      181 cgagggagcg gccaccggcg tcggcggcat cgtgcgcgat gtttactgca tgggcgccga
      241 cgtggtgggg gtgctcgacg cgctccgttt cggcgacctc accggtccca acggggaacg
      301 gtgccgggag atcgccgaag gggtggtcaa cggcatcgag cagtacgcca acgcgctcgg
      361 tgttcccaac ctcggcggcg atgcctggtt cgacccgggc tacgacgaca actgcctggt
      421 caatgtcgtg gccgtgggcg tggtgcgcga gaaggagatc atccggagtc gcgtgccgaa
      481 ggaagcacac gacgagccat acgacctgat cctcctcggc aagccgaccg acagctccgg
      541 cttcggcggc gcctcgttct cctcggcggt cctgtcggaa gacgacctcg aatcgaacaa
      601 gggggcggtg cagctgcccg atcccttcct gaaacgggtc atcgtcgagg ccacgcggaa
      661 ggcgctggcc ttctgccgcg agcggaaggc ggccatcggc ttcaaggacc tgggcgcggg
      721 ggggatcgcc tgcgtcacca gcgagctggc ggcccacggc ggcttcggca tggacgtcga
      781 gctcgaccgc gaaacgcagg agggcggccc gtacccgggc gaagtggtgg cctgcagcga
      841 aacgcaggag cgattcggcc tggtggtgcc cgcgcgcctg tccgaagaga ttctcgccat
      901 ctacaaccgc gatttcgagc tgccgcgtct ctacccgggc gcgggggcgc gggtcatcgg
      961 ccgcgtgcgg accgacgggc agtatcgcgt gagctatcgc ggcgaaactg tcgtcgacgc
     1021 cccggtcgat gtcatcaccg agggaatccg gtacgaccgc ccggcccggc cggcgcctcc
     1081 ggcggccagg ggccaggcgc tcccacccat cgacgacatg gccgacctga tcctccggat
     1141 ggtggcccat ccgaacgtcg cctcgcggga gtacatctat cgccattacg acaccgaggt
     1201 gcaggcgcgc gccgtcctgc gcccgggcga ggccgatgcc tcgctcttcc tgccgatccc
     1261 gggctcgccg gtcggtgtgg cggtgtcggt cgacggcaat cccgcctacg ggcggctcga
     1321 tccctaccgg gggggcctta ccgccgtggc cgaagcgatg cgcaacgtgg cggcggtcgg
     1381 ggcggtgccg cagtgcctca ccgactgcct gaacttcggc aatccggagg atcccgaggt
     1441 attccacgag ttccgcgagt cggtgcgcgg cctcggcgag gccgcccgcc ggctcggcct
     1501 gaagggagag cccggttcgc ccgtgccgtt cgtttcgggg aacgtgtcgc tctacaactt
     1561 ctcgacggcc ggtcgcgcca tcccgccatc gccgatcgtg gcctgcttcg gcatcctcga
     1621 ggactacagc aaggcggtgg ggctcaccat ccgcgagcca cgctcgctcc tcgtgctggc
     1681 gggtgagcgc cgcgccgaat tcggcggctc gatcgcggcc caggtgctcg gcgtcacggg
     1741 cggcgaacct ccgacggtcg attttga
//
LOCUS       DASVOD010000439         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_916029, whole genome shotgun sequence.
ACCESSION   DASVOD010000439 DASVOD010000000
VERSION     DASVOD010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916029"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VF720_10020"
     CDS             <1..799
                     /locus_tag="VF720_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7879736.1"
                     /translation="VCVPNHLHAPMTIAALNADKHVLCEKPFSRSPQEASKMVAAAEK
                     NGKCLMAAFNNRFRNDAQLLKTFIDKKEVGRIFYAKTGWLIKHASWDAESWKAQKRFA
                     GGGVLIDLGVQMLDLALWILGMPEVETVSASTARRPDIDEVESSAAAFLRLEGGATLT
                     LEVGWSLLMEKDFAYVNLFGEHGAALLNPFRLHKDMHGTLVNVTPATESARNVYKQSY
                     EAEIAHFVDCVRSGRKPSAPGTEAVTVLKVLEAIYKSAETGREVKLA"
     gene            807..>1803
                     /gene="lnt"
                     /locus_tag="VF720_10025"
     CDS             807..>1803
                     /gene="lnt"
                     /locus_tag="VF720_10025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX7879737.1"
                     /translation="MPVRSLRPPAWLLAILSGLLLAPAFPAASWGPLAFGAFVPVLIA
                     LVRERRGFWPSFRLGWLFGFAFFVVLLYWIPRLPPENVTVPLLLYPALVLAGLYCGIY
                     VAAWGGVVGWLARGRPAAIALLAPGFWVPIEIVRSSGVLGFPWGVIGGSQWTALDWIQ
                     AASIGGVHLLSFLIVLVNGCLAAALLSPGWRRPAWLAGALLLMVLPWQWGKGERARWE
                     EAMPMIPTVPVVLIQPNTGYDKWNAERRTAIITGLVQSTLEAARVTRDSTMIIWPETA
                     APTLLKWDEPNMALVRNAAEVTGRPLVTGFPDRTLDFPPETGGAARVRYYNVAGLF"
BASE COUNT          264 a          616 c          609 g          314 t
ORIGIN      
        1 cgtctgcgtt cccaatcacc tccacgctcc gatgacgatc gcggcgctga acgcggacaa
       61 gcacgtcctc tgcgagaagc cgttcagccg aagcccccag gaagccagca agatggtggc
      121 cgccgccgag aagaacggca agtgcctgat ggcggccttc aacaaccgct tccggaatga
      181 cgcccagctc ctcaagacct tcatcgacaa gaaggaagtg ggccggatct tttatgccaa
      241 gaccggctgg ctcatcaagc atgcctcctg ggacgccgaa tcgtggaagg cccagaagcg
      301 cttcgccggc ggcggcgtgc tcatcgacct gggtgtgcag atgctcgacc tggcgctctg
      361 gatcctcggc atgcccgagg tggaaacggt gagcgcctcc accgcccggc gccccgacat
      421 cgacgaggtg gagtcgagcg cggccgcgtt cctccggctc gagggggggg ccacgctcac
      481 gctcgaggtg gggtggtcac tcctcatgga gaaggacttc gcctacgtga acctcttcgg
      541 cgagcatggc gcggcgctct tgaacccgtt ccgcctccac aaggacatgc acggcacgct
      601 cgtcaacgtg acgccagcca ccgaatcggc gcgcaacgtc tacaagcagt cgtacgaggc
      661 ggaaatcgcg cacttcgtcg attgcgtccg ttcggggcgc aagccctccg cgccgggcac
      721 cgaggcggtc accgtgctca aggtgctcga ggcgatctac aagtcggccg agaccgggcg
      781 cgaagtgaag ctggcctagg agcaaggtgc ccgtacggtc gctccgtccg ccggcctggc
      841 tgctggcgat cctctccggc ctgttgctcg cgccggcctt tccggcggcg agctggggcc
      901 ccctggcgtt cggcgcgttc gtcccggtat tgatcgcgct tgtcagagag cgccgcggct
      961 tctggccgtc gttccggctc ggttggcttt tcggcttcgc cttcttcgtc gtcctccttt
     1021 actggatccc gcggttgccg ccggagaacg tcacggtgcc gctgctcctc tacccggcgc
     1081 tggtgctggc cgggctctac tgcggcatct atgtggccgc ctggggtggg gtcgtcggct
     1141 ggctggcccg cggacggccg gcggcgatcg cactcctggc gccgggtttc tgggtgccga
     1201 tcgagatcgt gaggagctcg ggcgtcctcg gatttccgtg gggcgtgatc ggcgggagcc
     1261 agtggacggc actcgattgg atacaggccg cctcgatcgg cggcgtgcac ctcctctcgt
     1321 tcctgatcgt cctcgtgaac ggttgcctgg ccgcggcgct cctctcgccg gggtggcgac
     1381 ggccggcctg gcttgccggc gcgctgctcc tcatggtgct cccgtggcaa tgggggaagg
     1441 gggagcgcgc ccgctgggaa gaggccatgc cgatgattcc cacggtgccc gtcgtcctga
     1501 tccagcccaa caccggctac gacaagtgga atgccgagcg acgcaccgcg atcattacgg
     1561 ggctcgtcca atccacgctc gaggccgccc gcgtgacgcg cgactcgacg atgatcatct
     1621 ggccggaaac ggccgctccc acgctcctca agtgggatga accgaacatg gcgctggtgc
     1681 ggaatgccgc ggaggtgacc ggacggcccc tcgtcaccgg tttccccgac cggacgctcg
     1741 actttccgcc ggagaccggc ggcgccgcgc gggtgcgcta ctacaacgtg gccggcctct
     1801 tcc
//
LOCUS       DASVOD010000440         5194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_706350, whole genome shotgun sequence.
ACCESSION   DASVOD010000440 DASVOD010000000
VERSION     DASVOD010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706350"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VF720_10030"
     CDS             complement(<1..1918)
                     /locus_tag="VF720_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879738.1"
                     /translation="MTACRVAVLLVLILSGVIGGPALAQPGGGQTPAPQDTLGRPVFG
                     FLRPTYSTSYNITDQEAAWDQNFKFTNTFGLFNLESNTGYNIKTDPNRTNFKATAGTS
                     DNRLRYNAFDVIPLSLSLIYNRDGTDDTNDRNRRSATNLGADGSYRIRLRRGMDLNLQ
                     GGAGLNARREHALGEFSETAVKEDGHDFTMGVGYGWINMMPGMSMRVDGDRTVATIKS
                     KTLVSDTLSQPADAKTNTRGTLNGNWTYLPFEALETRFVVSRNTTAESFVLVSRDTML
                     NGRQEEQSTLFQSISMDVTLRRPAPVVTDELNVKVGTTTNTNDRILEKERAYDKSGNS
                     FLVKGRKMLVGNQLDTKFEVTHDEDRSQIRPTATTDSRTLEQRLNRTVNPNLSLFASG
                     EVSLRAQKYTDPTSDKDIQKLRAEATVNWRATDSKYTATFGGRANQTDVVAVNSAQSV
                     SSSNEQNYGGSVNFTWRVSRKINLLQNYDYRLALTTYRFNPANDAIQRTRDIRTTLSY
                     DFIQPRPGISPTSVRFDLAHTYRYTEFGRYRLIEGERFFARTNDRYDQDLALGLGWTV
                     NAWLTLRTDEVFHRDDSVVRSSKDRRINRRLELLQTAKISRPLPGGGTIDASFTYRVR
                     QLKSETQTTRNPTAV"
     gene            complement(2145..3092)
                     /gene="rsmA"
                     /locus_tag="VF720_10035"
     CDS             complement(2145..3092)
                     /gene="rsmA"
                     /locus_tag="VF720_10035"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX7879739.1"
                     /translation="MRVTEANLELMRLRDEEGGNEPLHRELSKETQREMLRRYGVKPK
                     RRFGQNFLIDPQAIHKVIDLAGIDSADTVVEIGSGAGALTGRLADLAAAVHAVEIDTT
                     LAPILKQEFSGRNVIVHMTDIRKFEFDRLAEIAGQPLLVVGNLPYYLTTDILFTLMKF
                     GRYISRATLMVQKEYADRLLAEPGSKNYSALTVAARFRAEIESLYQLEPGVFYPAPQV
                     SSTLISLKPRRVPAAVVADEALFSGIVRASFGTRRKTLLNALMEKSRRPKNVVSDLIE
                     AAGVDPGRRGETLSVEDFARLAGVFHDAGVVVRAPGKMD"
     gene            complement(3089..3889)
                     /locus_tag="VF720_10040"
     CDS             complement(3089..3889)
                     /locus_tag="VF720_10040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX7879740.1"
                     /translation="MIDTHCHYLHPDFDPDRQEILQAVWDHGVTALLEVGYTPVLAQN
                     ALLWARRDIRVKVVAGIHPHETGRCTADDLDVIARLAEDSNVVAVGETGLDFYRDHAP
                     RDRQEEVFRRMIRLSRQLKKPLVLHVRDAYPEAIAILEEEGGGEFGGVFHCFQGDAQT
                     ARRARELGFMLGLGGAITYWSASRLDVISGIPLEAMLLETDAPWLCPAPRRGQRNDSR
                     HLDQVVEVIAAVTGHDGRTIVEATTANARGIFPGLGVGAAGAESGGRS"
     gene            complement(3972..>5194)
                     /gene="metG"
                     /locus_tag="VF720_10045"
     CDS             complement(3972..>5194)
                     /gene="metG"
                     /locus_tag="VF720_10045"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX7879741.1"
                     /translation="DIYKGHYEGWYCVGCEAYKQENDLIDGLCPIHHTKPDWLQEENY
                     FFALSRYQQKLLELYESNPSFLVPAERRNEVVNVVKGGLDDISITRANTTWGIPMPGD
                     PTHVIYVWFDALTNYLSAVGYGNDAPEGRALLERWWPADLHVVGKDITRFHCIIWPAM
                     LMSAGIPLPKQVLGHGWVTFKGEKMSKSLGNIVDPLDAAHKVGPDALRYYLLKEVPLD
                     RDGDFTWDLFIERYNTELANDLGNLVSRSVAMAEKYFGGELPAGDQFGPGGLDHPAAA
                     DLREVAAQARRDYEEAFDRHAVDEAIAAARTIVRRANQLIEETAPWTLAKDPGRKTEL
                     AAVMNALVESVRLSATLLWPVIPRKSEQILAALALPAPGPGGLGGAEWSPEAHRPTSP
                     LSRIEALFPRIDAI"
BASE COUNT          874 a         1627 c         1700 g          993 t
ORIGIN      
        1 tgacggctgt cgggttgcgg gtcgtctgcg tctcgctctt caactgtcgc acgcggtacg
       61 tgaagctggc gtcgatggtg ccgccacccg gaagagggcg cgaaatcttg gcggtctgga
      121 gcagctccag tcggcgattg atccggcgat ccttgctcga acgcacgacc gagtcgtcgc
      181 ggtggaacac ctcgtccgtc cggagcgtga gccacgcgtt gaccgtccaa ccgaggccga
      241 gtgccaggtc ctggtcgtag cggtcgttgg tccgggcgaa gaagcgctca ccctcgatca
      301 ggcggtaacg tccgaattcg gtatagcggt aggtgtgggc caggtcgaac cgcacggagg
      361 tcggcgaaat cccgggcctg ggctggatga agtcgtagct gagcgtcgtg cgaatgtccc
      421 gcgtgcgctg gatggcatcg ttggccgggt tgaaccggta cgtcgtcagg gccagccggt
      481 agtcgtagtt ctgcaacagg ttgatctttc tcgagacccg ccacgtgaag ttgaccgacc
      541 cgccgtaatt ctgctcattg ctcgagctga ccgactgggc gctgttcacg gccacgacgt
      601 cggtctggtt ggcccggccg ccgaacgtgg cggtgtactt cgaatcggtg gccctccagt
      661 tcacggtcgc ctcggcgcgc agcttctgga tgtccttgtc gctggtgggg tcggtgtact
      721 tctgggctcg cagcgacacc tcgccactgg caaagaggga gagattgggg ttgacggtgc
      781 ggttcaggcg ttgctccagc gtgcggctgt cggtggtggc ggtcggacgg atctgggacc
      841 gatcctcgtc gtgcgtcacc tcgaacttgg tgtcgagctg gttgccgacg agcatcttgc
      901 gtcccttgac caggaagctg ttccccgact tgtcgtaggc acgctccttc tcgaggatgc
      961 ggtcgttggt attggtagtc gtgccgacct tcacgttcaa ctcgtcggtg accaccggcg
     1021 cggggcgcct cagggtgacg tccatcgaga tggactggaa gagcgtcgac tgctcttcct
     1081 gccggccgtt cagcatggtg tcgcggctca cgaggacgaa gctctcggcg gtggtgttcc
     1141 ggctgacgac gaagcgggtt tccagcgcct cgaacgggag ataggtccag ttgccgttca
     1201 gcgtgccgcg cgtgttggtc ttggcgtccg ccggctggga gagggtgtcg ctcaccagtg
     1261 ttttcgactt gatggtggcc acggtccggt cgccatccac gcgcatcgac atcccgggca
     1321 tcatgttgat ccaaccgtat ccgacgccca tcgtgaagtc atggccatct tccttgacgg
     1381 cggtttcgga gaactccccc agggcatgtt cgcgccgggc attcaggccg gcgccaccct
     1441 ggaggttcag gtccattccc cgtcgcaacc ggatccggta ggagccgtcg gccccgaggt
     1501 tggtggcgct ccggcggttc cggtcgttgg tgtcgtccgt gccgtcgcgg ttgtagatga
     1561 ggctcaggga gaggggaatg acgtcgaacg cgttgtaccg gagccggttg tcgctggtgc
     1621 cggcggtggc cttgaagttc gtgcggttgg ggtcggtctt gatgttgtag cccgtattgc
     1681 tctccaggtt gaagagcccg aacgtattgg tgaacttgaa attctggtcc caggcagctt
     1741 cctggtcggt gatgttgtag ctggtggagt aagtcgggcg aaggaaaccg aaaaccggcc
     1801 ggcccagggt atcctgcgga gcgggcgtct ggcctcctcc cggctgcgcc agggccgggc
     1861 cgccgatcac cccgctcagg atcagcacca ggaggaccgc cacccggcat gcagtcatgg
     1921 cccgggcgcg atcacgcgac ggacacggct ttgccggaac tgtaaaggtg aggccggacg
     1981 ctctcatcgg tccttcgtct cgaaatggga cggaacgggg ccgcccacgg gcaagggcaa
     2041 ccgggtgtta accagatgag atggagcgtc ctccggtgga tcggggggct ttcgagggaa
     2101 caatcatatc agccggccgg cctcggggcc gaccggctgc tgattcagtc cattttgccc
     2161 ggggcccgga cgaccacacc cgcgtcgtgg aaaaccccgg ccaaccgggc aaagtcttcc
     2221 accgacaggg tttcgccgcg gcgcccggga tcaacaccgg ccgcctcgat caggtcgctg
     2281 accacatttt tcggccggcg ggacttttcc atgagggcat tgaggagggt cttccgccgg
     2341 gtgccgaagg aggcgcgcac gatgccggag aagagggcct cgtcggccac gaccgcggcg
     2401 gggacccgcc ggggcttcag gctgatgagc gtggagctca cctggggcgc cggatagaaa
     2461 acgcccggct cgagctggta gagcgactcg atctccgccc ggaaacgggc ggccacggtg
     2521 agcgcgctgt agttcttgct gccgggttcc gcgagcaggc ggtcggcata ctccttctgc
     2581 accatcaggg tggcccgcga aatgtagcgg ccgaacttca tgagggtgaa gaggatgtcg
     2641 gtggtcagat aataaggaag gttgcccacg accagcagcg gctggccggc gatctccgcc
     2701 aggcggtcga attcgaactt gcggatgtcg gtcatgtgaa cgatgacatt ccgcccggag
     2761 aattcctgct tgaggatcgg agccagcgtg gtatcgatct ccacggcgtg caccgcggcg
     2821 gccaggtcgg ccagccggcc ggtcagggcg cccgcgcccg aaccgatctc gacgaccgtg
     2881 tcggcgctgt cgatgcccgc caggtcgatg accttgtgaa tcgcctgcgg gtcgatcagg
     2941 aagttctgac cgaatcgccg cttcggcttg acgccgtaac ggcgcagcat ttcccgctgc
     3001 gtttccttgg acagctcgcg atgcagcggc tcgttgccgc cctcctcatc gcgcaaccgc
     3061 atcaattcca gattggcttc cgtgactctc aagatcttcc tcccgattcc gctccggcgg
     3121 cgcccacacc caaccccgga aatatgcccc gggcgttggc cgtcgtggcc tcgacgatcg
     3181 ttcgaccgtc gtggccggtg acggccgcaa tgacctctac tacctgatcg aggtgacgcg
     3241 aatcattgcg ctgtccgcgc cgtggcgcgg gacagagcca gggggcatct gtttcaagaa
     3301 gcatggcctc gagcgggatg ccggagatga catccagcct cgaagcactc cagtaggtga
     3361 tcgctccccc cagaccgagc atgaagccaa gctcccttgc ccggcgggcc gtctgtgcat
     3421 cgccctgaaa acagtggaag actccaccga attcaccgcc gccctcttcc tccaggatcg
     3481 cgatggcctc agggtaggca tcccgaacat ggagcacgag cggctttttg agttgccgcg
     3541 acagacgaat catccgccga aagacctctt cctggcggtc gcgaggtgca tggtcccgat
     3601 agaaatcgag acccgtttca cccacggcca ccacgttcga gtcttccgcc agacgagcaa
     3661 tgacatccag atcgtcggcg gtgcaccggc ctgtttcgtg cggatgaata ccggcaacca
     3721 ccttcactcg gatgtcacga cgggcccata gtagcgcatt ctgtgccagc acgggcgtat
     3781 aacccacttc cagaagtgcc gtaaccccat gatcccaaac agcttgtaaa atttcttgac
     3841 gatctggatc gaaatcgggg tggaggtagt ggcagtgggt gtcgatcaca ggcttccaac
     3901 accggagagt ggcgaaaatt ccgacaggtt aacgatttgc aacgtcgggg gtccgtccgg
     3961 atccgggaat gtcaaatcgc gtcgattcgg gggaaaagcg cttcgattcg tgacaggggt
     4021 gacgtcggcc ggtgcgcctc cggcgaccat tcggcacccc ccaacccccc cggtccgggc
     4081 gccggcagcg ccagggccgc caggatctgc tccgacttcc ggggaatgac cggccaaagg
     4141 agggtggccg agagccggac cgactcgacc agggcattca tgaccgccgc cagctccgtt
     4201 ttccggcccg gatccttggc caaggtccag ggggccgtct cttcgatcag ctggttggcc
     4261 cgccggacga tggtgcgcgc cgccgcgatg gcctcatcca cggcgtgccg atcgaatgcc
     4321 tcttcataat cccgccgcgc ctgcgcggcg acctcccgca gatcggccgc agccgggtga
     4381 tcgagccccc ccggaccaaa ctggtccccg gcaggcaatt cccccccgaa gtacttctcc
     4441 gccatcgcca ccgaccggct caccaggttc cccaggtcgt tggccagctc cgtgttgtag
     4501 cgctcgatga agaggtccca ggtgaaatcc ccatcccggt cgagcggcac ttctttgagg
     4561 aggtagtagc ggagcgcatc gggccccacc ttgtgggccg cgtcgagcgg atcgacgatg
     4621 ttgccgaggc tcttcgacat cttctcaccc ttgaacgtga cccacccgtg ccccaaaacc
     4681 tgcttcggca gcgggatccc cgcgctcatc agcatggccg gccagatgat gcagtggaat
     4741 cgggtgatgt ccttaccgac cacgtggagg tcggccggcc accagcgctc gaggagtgcc
     4801 cgcccctccg gagcgtcgtt gccgtagccg accgcgctca ggtagttggt gagggcgtcg
     4861 aaccagacgt agatgacatg cgtggggtct cccggcatcg ggatccccca ggtggtgttg
     4921 gcccttgtga tcgagatgtc gtccaagccc cccttcacca cattgacgac ctcattccgg
     4981 cgttccgccg gaacgagaaa tgagggattc gactcgtaca actcgagcag cttctgctgg
     5041 taccgggaga gggcgaagaa gtagttctct tcctgcagcc agtccggctt cgtgtggtgg
     5101 attggacaga ggccgtcgat gaggtcattc tcctgcttgt aggcctcgca ccccacgcaa
     5161 taccacccct cgtagtgccc cttgtagatg tctc
//
LOCUS       DASVOD010000441         4120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_499323, whole genome shotgun sequence.
ACCESSION   DASVOD010000441 DASVOD010000000
VERSION     DASVOD010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499323"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /gene="guaA"
                     /locus_tag="VF720_10050"
     CDS             <1..1022
                     /gene="guaA"
                     /locus_tag="VF720_10050"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX7879742.1"
                     /translation="TEIGTKLLRNFAVSVCGARTDWDIGNFIEESVERIRATVGTDRV
                     LCGLSGGVDSSVVALLIHRAIGKQLTCLFVDHGLLRVGDGDMVNHELRDHFGLDVRSV
                     DASGRFLAKLEDVSDPERKRKIIGEEFVRVFEEEARALGADMKWLAQGTLYPDVIESV
                     SVKGPSATIKTHHNVGGLPEKMDLKLLEPVRELFKDEVRDVGRRLGLPESVIGRHPFP
                     GPGLAVRILGPITERGVALVKAADAIFIEELRNSGEYDRIWQAFAVLLPVQSVGVMGD
                     YRTYENVAALRAVTSVDGMTADFADIPREVLARTASRIVNEVRGINRVVYDISTKPPA
                     TIEWE"
     gene            1081..3867
                     /locus_tag="VF720_10055"
     CDS             1081..3867
                     /locus_tag="VF720_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HEX7879743.1"
                     /translation="MLVVSLLAPAAVRPSGATVPVPVAPSTVAARAAMGGGVGPDRSF
                     RLPTSATSDDPFHYKLLVLLVEFRDVRQSTGDDRLTPAVVYDRIFGDRAGTLASYWKE
                     ASHGRLVLEGTVSNWVRVDSTYAYYCSEGSGNTGSGVDQLAYPHNVPRLVEESVQRLG
                     TGAAWPEYDVNADGVVDGLLVIHAGPGLEEAGEMPTDRRLVFLAHQFHTKTEIPVPGA
                     RVFDYAFVSADAGIGVAAHEFGHLLGLSDLYDTGIFAGALGPFGAGDWSLMATGALVN
                     DATMPTGLDAYSRMKLAFDLPDTIRVGAPPLARLFPQGDHSIVLLPAGRNPRESFLVE
                     ARGKSGLDAAQPGEGLLIWHVDEKRPGNLGPNHYRVALEQADGLDELGRPTGDRGDAG
                     DAWPPPTRFAHDTRPGSGNHDDEPSWVVIDRMERGAQGMSATLDIAPPATLRLQSYEV
                     AELVGDGDGRIEPGETGEFRFVVCNDGTVDDAPGTASLRILAGPDGPGAGSDSVMIPA
                     ARAGECVSPVAPLRFTLPLTAPEVSVDEAFLDIRGVSGADFFTPVVLAPPVGQHLSAN
                     PDEPPWLPPVPGAWRVDTTRFRTAPASWRLGPTSGTRYSANLDASYESPLLGVPTINP
                     RLEIWSAIDAETLSIGRAFDGGRLEIRVPPGDWQPLTPAGGWSHELEEGSGNALDGQP
                     VTSGHDAAFHRLVFDLAPWAGRAMNVRFRFASDPITVTDQSQTGWWLDDLELTAGDPP
                     PDLFAESTGDSVTVEWTTTIPNGWHVYRRPDVHRGAREPIAEVPPSTANGNVTHRVID
                     HPPAGTWIYSVRPLDDDGPLLEYDAEPVTIDIPRGTPSFAVTPNPWRLDAAPLTVEYV
                     IGNDAASAETVRVRMFDVAGRLVATLVNAGAESGHHEFRWPEGNAAPVPGVYFVRLDA
                     PGTEPMTARLVIVR"
     gene            3903..>4120
                     /locus_tag="VF720_10060"
     CDS             3903..>4120
                     /locus_tag="VF720_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879744.1"
                     /translation="MKRSMVMLAVMIVPSMFGMPAITHAAPSTSAATPAIRASDLPAL
                     YGAWSTADLAAWKELNVGNAVTIARAEG"
BASE COUNT          654 a         1390 c         1369 g          707 t
ORIGIN      
        1 ataccgagat cgggacgaag ttgcttcgca acttcgccgt gagtgtctgc ggcgcgcgca
       61 ccgactggga catcggcaac ttcatcgagg agtcggtcga acggatccgt gccacggtcg
      121 gcaccgaccg cgtgctgtgc ggcctgtcgg gcggcgtcga ttcgtcggtc gtggcgcttc
      181 tcattcacag ggccatcggc aagcaactca cctgtctctt tgtcgaccat ggcctgcttc
      241 gcgtcggcga cggcgacatg gtgaaccacg agctgcggga ccacttcggc ctcgacgtcc
      301 gctccgtcga cgcgagcggg cgtttcctgg ccaaactgga agacgtctcc gatcccgagc
      361 ggaagaggaa gatcatcggc gaggagttcg tgcgcgtctt cgaggaggag gcccgcgcgc
      421 tcggcgccga catgaagtgg ctggcccagg gaacgctcta tcccgacgtg atcgagagcg
      481 tgtcggtgaa aggcccctcc gccaccatca agacccacca caacgtcggc ggcctccccg
      541 agaagatgga cctgaagctc ctcgagcccg tgcgcgagct cttcaaggac gaagtgcgtg
      601 acgtcggccg ccgcctggga ctgccggaaa gcgtcatcgg ccgtcatccg ttccctggcc
      661 ccggccttgc ggtgcgcatc ctggggccga tcaccgagcg aggcgtggcc ctcgtcaagg
      721 ccgccgacgc catcttcatc gaggaattgc gcaattccgg cgagtacgac cggatctggc
      781 aggccttcgc ggtgttgctc cccgtgcaat cggttggggt gatgggggac taccgcacgt
      841 acgagaacgt ggcggccctc cgcgccgtca ccagcgtgga cggcatgacg gccgacttcg
      901 ccgacatccc ccgcgaggtc ctggccagga ccgccagccg catcgtcaac gaagtgcgtg
      961 gcatcaatcg cgtggtgtac gacatctcca ccaagccacc ggccacgatc gaatgggaat
     1021 agatggagta atggaatgat tcgaccgacg agtcgcgcga tccccccccg gatatggagc
     1081 gtgctcgtcg tgagtctcct ggcaccggcc gccgtccgcc cgtcgggcgc cacggtgccg
     1141 gtgcccgtgg cgccgtcgac agtcgccgcg cgtgccgcga tgggaggcgg tgttggcccc
     1201 gatcgatcct ttcgactccc gacgtccgcg acgtcggacg atccctttca ctacaagctt
     1261 ctcgtgctcc tcgtcgagtt ccgggacgtc cgccaatcaa caggtgacga ccggctcacg
     1321 ccggccgtcg tttacgaccg catcttcggc gaccgtgccg gcacgctggc ctcatactgg
     1381 aaggaagcca gccacgggcg gctcgtgctc gaagggacgg tgtcgaactg ggtgcgcgtg
     1441 gactcgacct acgcgtacta ctgcagtgaa gggtctggta acacggggag cggcgtcgat
     1501 caactcgcgt acccgcataa cgtgccgcgg ttggtcgagg aatcggtgca gcgcctgggc
     1561 accggcgccg cctggcccga gtacgacgtg aacgcggatg gcgtggttga cggcctgctc
     1621 gtgatccacg cggggccggg gcttgaagag gcgggggaaa tgccgaccga tcgacgcctc
     1681 gtctttctgg cccaccagtt tcatacgaag accgaaatcc ccgtgcccgg cgcgcgcgtg
     1741 ttcgactacg cgttcgtgtc ggccgacgcc ggtatcggcg tggcggcgca cgaattcggc
     1801 catctgctcg ggctctcgga cctctacgac accggaatct tcgcgggggc gctcggcccc
     1861 ttcggcgcgg gagactggtc gttgatggcc accggcgcgc tggtgaacga cgccaccatg
     1921 ccgacggggc tcgatgctta cagccggatg aagctggcgt tcgatctgcc cgacacgatc
     1981 cgcgttggcg caccacccct ggcgcgactc ttcccgcagg gcgatcactc gatcgtgctc
     2041 cttcccgccg gtcggaatcc ccgggagtcg tttctcgtcg aggcgcgcgg gaagtcgggc
     2101 ctcgacgcgg cccagccggg ggaggggctt ctcatctggc atgtcgacga gaaacggccc
     2161 ggcaaccttg gtccgaacca ctaccgcgtg gcactggagc aggccgatgg gctcgatgaa
     2221 ctgggtcgac cgaccggcga ccgcggtgat gctggtgatg cctggcctcc tccaactcgc
     2281 tttgcgcacg atacgcggcc gggctcgggg aatcatgacg atgaaccgtc gtgggtcgtg
     2341 atcgaccgga tggagcgggg agcccagggg atgagcgcga cgctcgatat cgctcccccc
     2401 gccacgttgc gacttcaatc ctacgaagtc gcggagctcg tcggcgatgg cgacggccgg
     2461 atcgaacctg gcgagaccgg ggaattccgc ttcgtcgtct gcaacgacgg caccgtcgac
     2521 gacgcacccg gcacggcctc gctacgcatc ctcgccgggc cggatggacc gggtgccggg
     2581 agcgactccg tcatgattcc tgcggcgcgc gcgggtgagt gcgtctcacc ggtcgcgccg
     2641 ctccgcttca ctctcccctt gaccgcaccc gaggtttcgg tggatgaggc ttttcttgat
     2701 atcaggggcg tgagcggcgc tgacttcttc accccggtgg tcctcgcgcc cccggtcggg
     2761 cagcacctct ccgccaatcc ggatgagcct ccatggctgc cgcccgtgcc aggggcatgg
     2821 cgtgtcgaca cgacacgctt tcgaacggct ccagcgtcct ggcgacttgg accgacctcg
     2881 gggacgcgct actcggccaa tctggatgcc tcatacgaat ctcccttgct cggcgtgccg
     2941 acgatcaatc cccgcctcga aatctggtcc gccatcgacg ccgagacatt gtcgatcgga
     3001 cgcgcgttcg acgggggccg gcttgaaatc cgggttccac cgggcgactg gcagccgctg
     3061 acgccggccg gcggctggtc gcacgaactg gaggaaggtt cgggcaacgc cctggacggg
     3121 cagccggtga cctccggcca tgacgccgca ttccatcgac ttgtcttcga cctggccccg
     3181 tgggcaggtc gggccatgaa cgtgcggttt cgttttgcca gcgaccccat caccgtgacc
     3241 gaccagagcc agaccggttg gtggctggac gatctcgagt tgacggcggg ggatccgccg
     3301 cccgatctgt ttgccgagag cacgggcgat tcggtgaccg tggaatggac gaccacgatc
     3361 ccgaacgggt ggcacgtcta tcgtcgaccc gatgtccacc gtggtgcgcg cgagcccatc
     3421 gccgaggttc cgccatcgac cgccaacggt aatgtcaccc accgcgtcat cgatcatccc
     3481 cccgccggca cctggatcta ttcggtccgc cccctcgatg acgacggtcc gttgctcgag
     3541 tacgacgcgg agcccgtcac gatcgacatc ccgcgcggga ccccgtcctt tgccgtcacc
     3601 cccaacccct ggcgactgga cgccgcgccc ctgaccgtcg aatatgtaat cgggaatgat
     3661 gcagccagtg cggagactgt ccgtgtccgg atgttcgatg tcgccggcag gctggtggcc
     3721 acgctggtca atgcgggcgc ggagtcgggc catcacgaat tccggtggcc ggagggcaac
     3781 gcggcgccgg tgccgggcgt ctacttcgtt cgattggatg cgccgggcac cgaaccgatg
     3841 acggcgcgtc tcgtgatcgt gcgatgagga cggggataag ggctcgcggg ggaatgatgg
     3901 cgttgaaacg atcaatggtg atgctggccg tgatgatagt gccgtcgatg ttcggcatgc
     3961 cggcgatcac ccacgcggcc ccttcgacct cagccgccac ccccgccatc cgcgcctccg
     4021 atctccccgc gctgtacggt gcctggtcca ccgccgatct cgccgcctgg aaggaactga
     4081 acgtcggcaa cgcggtcacc atcgcccgcg cggaggggga
//
LOCUS       DASVOD010000442         5481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_186556, whole genome shotgun sequence.
ACCESSION   DASVOD010000442 DASVOD010000000
VERSION     DASVOD010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5481
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186556"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..677)
                     /locus_tag="VF720_10065"
     CDS             complement(<1..677)
                     /locus_tag="VF720_10065"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX7879745.1"
                     /translation="MRRFAVFLLVLTVSVASPAPLPAQPPAEQQDPAPAPLLLDDHSG
                     GPVLGPERIRVDAEGKTFVDPSSDTVFPAAMTVVARSGDVIHLTATGSGVRKKLIYKV
                     YAAALYVDALAPLGTVPVNTIASSDIARRMVMVFLRDVEGKRLKEGMRESFENTIYRG
                     KPGPELSAELDTFFSWMDAGIKKGQTIELTYLPGEGLYTTVAGVMHPVVTRPDIARSI
                     WLNWVGV"
     gene            complement(681..1076)
                     /locus_tag="VF720_10070"
     CDS             complement(681..1076)
                     /locus_tag="VF720_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX7879746.1"
                     /translation="MSHPETGQGRRWLRLGALSGFTAVALGAFGAHALKSRLTPDMLA
                     IFETGVRYQMYHALALLAVGILANKPGTAAGRSGLAFALGTLVFSGSLYLLALTGQRW
                     LGAITPIGGLLYLAGWLNLVMVFGRRTPE"
     gene            complement(1073..3955)
                     /locus_tag="VF720_10075"
     CDS             complement(1073..3955)
                     /locus_tag="VF720_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7879747.1"
                     /translation="MPALAAAVLPSLAHLANTHASQSPTFSALPVEEIGAISFRRAHP
                     GWDGRGVVVGVLDSGIDLGVAGLDRVPTGGRKILDARDFSGQGDVKLTRARFDTRDGR
                     PALVDTKKRAVFGVDRLPATSPDSTYWLGFVDEKEWRFSSVKDLNLNGANDDRFAVLA
                     FAPTADAADSQWVALVDLDADGQLDDEEPIRSYGVSGQLVRFRSSSDRETLHPIAGGI
                     TIRPGDKVVELHLADNGHGTHVAGIATGYRIGDNDGLDGVAPGAHLLSLKIGNNAMSG
                     GATTTESVKKAVNWAIEWAEDRKVPLVFNMSYGIESVREGQSDVDKFIDKTLLEHPQV
                     VFVVSNGNNGPGLSSAGTPSAAAYAISVGNMSSDEAARAIGGQGLKRDMMNFGSSRGG
                     EAAKPTVVAPGTVLSTVPPFDGGEVKNGTSMSSPHVAGAAALLLSAAGAEKVPWTFAT
                     VSQALAGTARPLPGYTLLDQGGGLVQIEPAWNALRAMTARSTSTLLVTKVEVTNPGYP
                     SGKGPAAFWRNSGWFPAPPRETTVTLTPQFADAATPDARAAMLTDFDLEADASWIRLD
                     RGSIHLKGDGTSTFDIEYDPAALKSPGLYTGRVRGFPRGMGGARIAAFEAWQTVIIPW
                     RFDGTDGTRTWSGKGMEPGGIERIFIEVPSGASALSLSLEAEESPGGQCRVVLFDPEG
                     HSHGTFAGWADPANGATHEQILSGKDLVAGTWEAIVGAASANRRDAAWKLEARLSQLE
                     CRPDTLARFAMPPGEATTAKATVVNRSDRLFAGRAEGSIQGWRRERTIHVVDDDTYEM
                     PFTLPPDIDQVTFEASFEESLYQKTSDIVIGILDKNGVALNRTSFDVLSGSITFANPA
                     KGGGESGSYRLELRPGFVHAASADDWSFRLVESWRRRDRDAVKVTANDSEALTFYPGA
                     PVELEVTMPLTPREAPDGAFNYGEIVIREEATKKTGVVWPIRLRGR"
     gene            complement(4159..5190)
                     /locus_tag="VF720_10080"
     CDS             complement(4159..5190)
                     /locus_tag="VF720_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX7879748.1"
                     /translation="MTTLLPPGGWIILTGALTAISCALIGCFLVLRRMAMLGDAISHA
                     VLPGIVLAFLWTGSRSSPAILVGAGALGVLTAVIAEFLHKTGRLQADAAIGVTFTWLF
                     ALGVILVSALAGQVDLDQDCVLYGEIAYVPWDTFIWRGMDLGPRAVWTLGGLTVLMFG
                     FVTLGWSRLKLISFDPALAASLGVSVGLWHYLLMGAVSMTTVASFEIVGAILVVAMLV
                     APAATAYLLTDRLATMLGLAAGFGVLSAALGYGMAKALNASIAGSIGVVAGVLYGLAL
                     VFGPRHGILARKRALKAALGVAAVAGAAWSSLAPGTAAAYDLLHPRPREEMRELATDR
                     PDLTASPPA"
     gene            complement(5187..>5481)
                     /locus_tag="VF720_10085"
     CDS             complement(5187..>5481)
                     /locus_tag="VF720_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HEX7879749.1"
                     /translation="GFVVGVAAEGAAGGSAWRLTASGQVRAARLVRLHRLWEIYLTER
                     LRLPIDHVHGDAEDVEHILTPELEAELERILDRPRPDPHGRPSPYPEAISEGP"
BASE COUNT          888 a         1820 c         1962 g          811 t
ORIGIN      
        1 tccaccccca cccagttcag ccagatgctg cgggcgatgt cggggcgcgt gacgaccggg
       61 tgcatgacgc cggccaccgt ggtgtagagc ccctcacccg gaaggtaggt gagctcgatc
      121 gtctggcctt tcttgatgcc ggcgtccatc cacgagaaga aggtgtcgag ttcggcggag
      181 agctcaggcc ccggcttccc cctgtaaatg gtgttctcga aactctcgcg catcccctcc
      241 ttcagcctct ttccctccac gtcgcgcagg aaaaccatga ccatgcggcg cgcgatgtcg
      301 gacgaagcaa tcgtgttgac cggaacggtt ccgaggggcg ccagcgcgtc gacgtagagt
      361 gctgcggcgt acaccttgta gatgagcttc ttgcggacgc cggagccggt ggccgtgagg
      421 tggatcacgt cgccggaacg ggcaacgacg gtcatggcgg cgggaaagac tgtgtcgctc
      481 gaggggtcga cgaacgtctt cccttcggca tccacgcgga tgcgctccgg gccgaggacg
      541 gggccgccgg agtggtcgtc gaggaggagc ggggccggcg cgggatcctg ctgttctgcc
      601 ggcggctggg cgggtagcgg agcgggtgac gcgacggaaa ccgtgaggac caggagaaag
      661 accgcgaaac gacgcatcaa ctactccggg gtgcggcggc cgaacaccat tacgaggttg
      721 agccatcccg ccaggtacag gagcccgccg atcggcgtga tggcgcccag ccagcgctgg
      781 ccggtcaacg cgagcaggta gagcgaaccc gagaagacca gcgtgccgag ggcgaaagcg
      841 aggccgctcc ggccggcggc ggtgccgggc ttattggcca ggatgccgac ggcgagcagc
      901 gcgagcgcat gatacatctg gtagcgcacc ccggtttcaa agatcgcgag catgtcgggc
      961 gtgaggcggc tcttcagggc atgggcgccg aaggccccaa gcgccaccgc ggtgaagccg
     1021 gagagggcgc ccagccggag ccagcgccgg ccctggccgg tctcagggtg gctcatcgcc
     1081 cgcgaagacg gatgggccac acgacgccgg tcttcttcgt cgcctcttcc cggatcacga
     1141 tctcgccgta gttgaaggcc ccgtccggcg cctcgcgcgg cgtgagcggc atcgtgacct
     1201 cgagttcgac cggtgcgccc gggtagaacg tgagcgcctc cgagtcgttg gcggtgacct
     1261 tgaccgcatc gcgatcacgc cggcgccacg actccaccag ccggaacgac cagtcatccg
     1321 ccgaagcggc atgcacgaag cccggtcgca attcgagacg atacgacccg ctctcgcctc
     1381 cccctttcgc ggggttggca aaggtaatgc tgccgctgag cacgtcgaac gacgtgcgat
     1441 tcagcgccac gccgttcttg tcgaggatgc cgatcacgat atcgctggtc ttctggtaga
     1501 gcgattcttc gaagctcgcc tcgaatgtca cctggtcgat gtcgggggga agggtgaacg
     1561 gcatttcgta cgtgtcgtcg tcgacgacgt ggatcgtccg ctcgcgccgc cacccctgga
     1621 tcgatccctc ggcgcgaccg gcgaacaggc gatccgagcg gttcacgacg gtggctttcg
     1681 cggtggtggc ttcgccgggc ggcatggcga aacgggccag cgtgtccggc cggcattcca
     1741 gctggctgag gcgagcctcg agtttccagg cggcatcccg ccggttggcg gatgcggcgc
     1801 cgacgatggc ttcccaagtg ccggccacca ggtctttccc ggagagaatc tgctcgtggg
     1861 tcgcgccgtt ggcgggatcg gcccagccgg cgaacgtccc gtgcgaatgt ccttccggat
     1921 cgaacagcac cacgcggcac tggccgccgg gcgactcttc ggcttcgagc gacagggaga
     1981 gcgccgacgc gccggacggc acctcgatga agatccgctc gatgccaccc ggctccatgc
     2041 ccttgccgct ccaggtgcgc gtcccgtcgg tgccgtcgaa gcgccaggga atgatcacgg
     2101 tctgccaggc ttcgaaggcg gcgatcctgg ccccccccat gccgcgcggg aagccgcgca
     2161 cgcgccccgt gtagaggccg ggcgatttca gcgccgccgg gtcgtattcg atgtcgaagg
     2221 tgctcgttcc atcccccttc aggtggatgg agccgcgatc gaggcgaatc cacgacgcgt
     2281 cggcttcaag atcgaagtcc gtgagcatgg cggcgcgcgc gtcgggagtg gcggcatcgg
     2341 cgaactgcgg cgtgagcgtc accgtggttt cgcgcggcgg cgccggaaac cagccgctgt
     2401 tccgccagaa tgcagccggc ccctttcccg aggggtagcc cgggttggtg acctcgacct
     2461 tcgtcacgag aagcgtggac gtggagcggg ccgtcatcgc ccggagcgcg ttccaggccg
     2521 gctcgatctg cacgagcccg cccccctggt cgaggagggt gtaaccgggg agcgggcgcg
     2581 ccgtgccggc cagcgcctgg ctcacggtgg cgaaggtcca cggcaccttc tcggcaccgg
     2641 cggccgaaag gagcagcgcg gccgcgccgg ccacgtgcgg cgacgacatg ctggtgccgt
     2701 tcttcacctc gccgccatcg aacggcggca cggtcgagag caccgtgccg ggcgccacca
     2761 cggtgggctt ggcggcctcg ccgccgcgcg aggaaccgaa gttcatcatg tcgcgcttca
     2821 gcccctggcc gccgatggcc cgcgcggcct cgtcggagct catgttcccc accgagatgg
     2881 cgtacgccgc ggcgctcggg gtgccagcgc tcgagaggcc cggcccgttg ttgccgttcg
     2941 acacgacgaa gaccacctgc gggtgctcga gcagcgtctt gtcgatgaac ttgtcgacat
     3001 ccgactgccc ctcgcgcacg ctctcgatgc cgtacgacat gttgaagacg agcggcacct
     3061 tgcgatcttc cgcccattcg atcgcccagt tcacggcctt cttcacgctc tcggtggtcg
     3121 tggcgccgcc cgacatcgcg ttgttgccga ttttgagcga gagaaggtgg gcaccgggcg
     3181 ccacgccgtc gaggccgtcg ttgtcgccga tccggtaacc cgtggcgatg cccgccacat
     3241 gcgtgccgtg gccgttgtcg gccaggtgca gctcgacgac cttgtcgccg ggacggatgg
     3301 tgatgccgcc ggcgatcgga tggagcgttt ccctgtcgct cgaggagcga aaccggacga
     3361 gctgacccga cacgccgtag gagcggatcg gttcttcgtc atccagctgg ccgtcagcgt
     3421 ccaggtcgac cagtgccacc cactgggagt cggcggcgtc cgcggtcggg gcgaaggcca
     3481 gcacggcgaa gcggtcgtcg ttggcgccgt tcaggttcaa atccttgacg ctcgagaagc
     3541 gccattcctt ctcgtccacg aagccgagcc agtaggtgga gtcggggctg gtggccggga
     3601 gccggtcgac accgaagacg gcgcgcttct tcgtatcgac cagggccgga cggccgtcac
     3661 gtgtgtcgaa gcgtgcgcgc gtgagcttca catccccctg gccgctgaag tcgcgagcgt
     3721 cgaggatctt gcgcccgccg gtcggcaccc ggtcgagccc cgcaaccccg aggtcgatcc
     3781 ccgaatcgag cacgccgacc acgacgccgc ggccatccca cccgggatgc gcccgccgga
     3841 acgagatggc gccgatttcc tccacgggga gcgcgctgaa agtgggggat tggcttgcgt
     3901 gcgtgtttgc caggtgggcg agagacggga gaacagcggc ggcgagggcc ggcaagagtg
     3961 cgcttcgcca cgcggggccg cgattgcggc ggggggaccg ggaaggcatc ggagaaagat
     4021 gcctgaaccg ccgcgcgaat tccagcgcgc cctaccgccg gaacgccatc ccgaggaagg
     4081 tctccacgtc ctgcgcgctg tcggtgagcc cgagccgcac gccgccgtcg aagcggagat
     4141 cgcggtttgc ggacaccgtc atgccggtgg cgaagcggtc aggtcgggac ggtcggtggc
     4201 cagttcccgc atttcttcgc gaggccgcgg atggaggagg tcgtacgcgg cggcggtgcc
     4261 cggtgcgagg ctgctccatg ccgcgccggc aacggccgcc accccgagcg ccgccttgag
     4321 cgcgcgcttc cgggccagga tgccgtggcg cgggccgaag acgagcgcca ggccgtacag
     4381 gacgccggcc acgactccga tcgatccggc gatcgacgcg ttgagtgcct tggccattcc
     4441 gtaaccgagg gccgccgaca gcacgccgaa gccggcggcc aggccgagca tggtggccag
     4501 gcggtcggtg agcaggtagg cggtggcggc cggggccacc agcatggcca ccacgaggat
     4561 ggctcccacg atttcgaacg aggcgacggt ggtcatcgat acggctccca tcaacaggta
     4621 gtgccagagg cctaccgaga cgccgagcga ggcggccagg gcgggatcga aactgatgag
     4681 cttgagacgg ctccagccga gcgtgacgaa gccgaacatg aggaccgtga gcccgcccag
     4741 cgtccagacg gcgcgcggac cgaggtccat tccccgccag atgaacgtgt cccatgggac
     4801 gtacgcgatc tcaccgtaaa ggacgcagtc ctggtcgagg tcgacctggc cggccagcgc
     4861 gctcaccagg atgacgccca gcgcgaagag ccaggtgaag gtgacgccga tggcggcgtc
     4921 ggcctgcagg cgccccgtct tgtggaggaa ctcggcgatg acggccgtca gcacgccgag
     4981 agcgcccgcc cccaccagga tggccgggga cgagcggctg ccggtccaga ggaacgccag
     5041 cacgatgccg gggagcacgg cgtggctgat ggcatccccc agcatggcca tgcggcgcag
     5101 caccaggaag cagccgatca gcgcgcagga gatggcggtg agcgcgccgg tcagaatgat
     5161 ccagccgccg ggtggcagga gagtcgtcac ggtccctccg agatggcttc gggataagga
     5221 ctgggccgcc cgtgcggatc cgggcgcggg cgatcgagga ttcgttcgag ctcggcttcg
     5281 agctcgggcg tgaggatgtg ctcgacgtcc tcggcatcgc catggacgtg atcgatcggg
     5341 agccgcagcc gctcggtgag ataaatctcc cagagccgat ggagtcgaac caggcgggcg
     5401 gcccgcacct gacccgaggc ggtgaggcgc caggccgagc cgcccgcggc cccttcagcg
     5461 gcgaccccga cgacgaaacc c
//
LOCUS       DASVOD010000443         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_706423, whole genome shotgun sequence.
ACCESSION   DASVOD010000443 DASVOD010000000
VERSION     DASVOD010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706423"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VF720_10090"
     CDS             complement(<1..1335)
                     /locus_tag="VF720_10090"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7879750.1"
                     /translation="MHRCAASLSLLTLLVALFAPIGFAHGPLHDDIRRLTGRIAAEPS
                     CAECLLRRGELYRLDENPAAAKLDYDAAERIDPFLPGLKLCRAALALDDGANELALAF
                     ASSQLASHPDDEAALSLRAEIYDRLDRTASADADRARLAVLMALPRPGTTPETGAATA
                     AATLIGRGASWKYLATATDQGSGWRATGFADGGWPAGGAPLGFGESYIVTSVPYGGNA
                     SNKWRTTYFRKSFNQSDATASLVSLIMTASYDDGFVAYLNGTEVARRGLTGTVTWSTL
                     AASHEAGLYETIDLTAFKNLLIVGNNVLAIEVHQTSASSTDLVWDADLVSSNAASVTR
                     GPYLQVLRPTGVTVRWRTNLAGDSRVRWGTSPAMLTNNTDVATVTTEHEVVVNGLTPG
                     TRYWYSVGSTAAAMAGDSTYTFRTAPTGSEPTTVWVVGDSGEPPGAGLVRDGY"
     gene            complement(1375..>1560)
                     /locus_tag="VF720_10095"
     CDS             complement(1375..>1560)
                     /locus_tag="VF720_10095"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879751.1"
                     /translation="AHQRRGIGTLLLVAGEAALVARGADTIVVTTANHRADAHAFYEK
                     SGYTFTGRRYVKAAHRP"
BASE COUNT          226 a          522 c          566 g          246 t
ORIGIN      
        1 gtagccgtcg cgcacgaggc cggcgccggg cggctcgccg gaatcgccca ccacccagac
       61 ggtggtcggt tcactgccgg tgggtgccgt ccggaacgtg tacgtggagt cgccggccat
      121 ggccgcggcg gtggagccca cgctgtacca gtagcgcgtg ccgggcgtga ggccgttcac
      181 gaccacctcg tgctcggtcg tcaccgtggc cacgtcggtg ttgttcgtca gcatggccgg
      241 cgatgttccc caccgtacgc ggctgtcgcc ggccaggttc gtgcgccagc gaacggtcac
      301 gccggtcggg cgcaggacct gtaagtacgg tccgcgcgtc acgctggccg cgttcgaact
      361 gacgaggtcg gcatcccaca cgagatccgt cgagctggcg ctcgtctggt gcacttcaat
      421 ggccagcacg ttattgccga cgatgagcag gtttttgaat gcggtcaggt cgatcgtttc
      481 gtagagcccg gcttcgtggc tggcggcgag cgtggaccag gtgacggtgc cggtgagccc
      541 gcgacgtgcc acctcggtgc cgttgaggta ggccacgaag ccgtcgtcgt agctggcggt
      601 catgatcagg ctcaccagtg aggccgtcgc gtcggactgg ttgaacgact tgcggaaata
      661 ggtggtgcgc cacttgttgc tggcgtttcc gccgtacgga acgctggtca cgatgtacga
      721 ctcgccgaat ccgagcggag cgccgcccgc gggccagccg ccgtcggcaa aaccggtggc
      781 ccgccacccc gacccctggt cggtggcggt ggccaggtac ttccaggagg cgccgcgacc
      841 gatgagggtg gcggccgcgg tcgccgctcc ggtttccggc gtcgtgccgg gcctcgggag
      901 cgccatgagg accgccagcc tcgcccggtc cgcgtcggcg ctggccgtgc ggtcgagccg
      961 gtcgtagatc tcggcccgca gcgacagggc cgcttcgtca tcgggatgcg aggccagctg
     1021 gctcgacgcg aacgccagtg ccagctcgtt cgccccgtcg tcgagggcca gggccgcccg
     1081 gcagagcttg aggcccggca ggaagggatc gatccgctcg gccgcgtcgt agtcgagctt
     1141 cgcggccgcc gggttctcat ccagccgata gagctccccg cgccgtaaca ggcactcggc
     1201 gcacgaaggc tcggccgcga tccggccggt cagccggcgg atgtcgtcgt gcaggggccc
     1261 atgcgcgaag ccgatcgggg caaatagcgc aacgagaagg gtgaggaggg agagtgaggc
     1321 agcgcaccgg tgcatgatgc caacgagtct atcacgcgcc ccgcgggcaa ccggtcaggg
     1381 tcgatgcgcc gccttcacgt accgccgtcc cgtgaacgtg taaccactct tctcgtagaa
     1441 cgcgtgcgcg tcggcgcgat gattggccgt cgtcaccacg atcgtatcgg caccacgcgc
     1501 gacgagcgcc gcctcaccgg cgacgagcag cagcgtgccg atgccgcggc gctggtgcgc
//
LOCUS       DASVOD010000444         9455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_238158, whole genome shotgun sequence.
ACCESSION   DASVOD010000444 DASVOD010000000
VERSION     DASVOD010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9455
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238158"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="VF720_10100"
     CDS             complement(<1..427)
                     /locus_tag="VF720_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879752.1"
                     /translation="MSAIPAASTAATVARPRFFPRDMAALAFLLVAAPAAVWLGIRIG
                     QPGLFPVLTALPAILVFLDRINAGRLSSALGLMVAWSTALSVAVILNTRWNPAGTETV
                     IWNGATYRDEMLAWVRTGVGAEGSPRLFLPQHALHYGVFL"
     gene            complement(424..1329)
                     /locus_tag="VF720_10105"
     CDS             complement(424..1329)
                     /locus_tag="VF720_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX7879753.1"
                     /translation="MSLSKRAALYWEFARPFTLVAPALGILSGGVTAFGAHPHETFAW
                     HHVVNIGLGTLMAGVLNAASNGLNQIHDLTNDRVNKPGRPIPSGRMSIREAGWFTWIT
                     FGVALLLAALVNWQCFALALAATIFTWAYSAPPLRTKREGIWANITIAIPRGVLLKVA
                     GWSTVKSIFGAEPWFIGSIFGLFLLGAATTKDYSDIEGDKADGCRTLPIQYGVTKSAW
                     MISPSFVLPFLLLPLGVYLKVLTGNPIFLTLLGVGLALWGGYVCWLILRRPEELASTE
                     NHPSWTHMYWMMFVTQIGFMVAYLA"
     gene            1593..5918
                     /locus_tag="VF720_10110"
     CDS             1593..5918
                     /locus_tag="VF720_10110"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX7879754.1"
                     /translation="MTDCARRPTFPPARMIRTIEIAALILLASFSWVAPVARAEEATP
                     LKLVAADATGVTLEYTAPEPRFTPTETPFGNFDRIEMDGHGTSGFLGQPSLPETGTGI
                     GVPEGAEPRLTFEILESIELTGHRPLPMGDAPTAPGSTPTRVDRSAFDAAAYEPGNEP
                     VREPIAYAELGQLRHQRIGQVSVRPLRLHDGDQLTWIRRARIRVDFRMPSRAANAATL
                     PVGVEPAWEEAYRGAMINYESARSFRSRPMPNPAGRGGDRSLLESTDAAIDLARAIDG
                     NPEMRLFVAKTGLYYLSFADLSAKGWPDNVAIGQVSFFEKSYNAADPANPLIRDIPVT
                     IEEGTTGTPGIFDGDDTIYLYVMNFADRTQATPQFTRFSNLQAYWLSWRAAGGSRMTS
                     DPGWFESASVTTPSSYVMETHYEQNPYYLTDRVEEESYQGNETFHPKIEGYFWLEGIV
                     YRDSLQFQTPGRDATRPYRFRARWQGLFAGNHHAKVWQKTGCGANAPDTLIGRTPPYM
                     NIVNRGGFCSGCAYIMDTGYRVPGARLNDGCNEIRLQGNGVANGDTTSEGSGAYFDWF
                     EVDYPRRYVASGGTLRFTSSNATGDVEFVISDLRESDIRVFDITDSLAPRLVAIDPSK
                     IVQTQGAPNRRWQATMRVRVTGGQRTYVLWEYSQIPNVSTSVANGRMPKDQGTTNLAL
                     DTPDQLTTDTGANVLVICHPDFRTTFEPWKALREGQGNRLRFVSPQDIYDQFSGGVKT
                     PYAIRLWLRYVYQNWTVAPDDVVLIGDASEDYRSDVTGNPKFKSDPDWVPTMMIYGPV
                     PNGAGRREKIGTDHWYIGSLGPGQGEFDMLPEMYIGRISVNSTTDAQIMVDKLVAYDN
                     YTANDTWRNRGLIVSDDQFSSPLAGFNDYRYQPTELIFEEVGDSLAHIYNGLSCYNGF
                     EATHWRLDDYLTALNRPKPPCPPNNPTCQGNALAAQAEARRTATPAWVEESSKGHLFQ
                     IYSGHANAAVMATELFVEFRGDRLSPDARITEQLTNYGKPFIFFGVACHLNEFESYNE
                     GNTKMCLSESMLFLANRGAIASIASTGFEWLYSNEGVELYLGKVMFAEHPRDPLTGRP
                     RRILAESMYAALIRLITERPNDGFLYNDTIRSYGLLGDPTMRIDMAPPILAVTVDGAP
                     VEPNTAVRAAPGADAVTVQVKVADDVDDKADSFAITAGETAVPSSEWTSTPFQTSDLG
                     ACHGVRIDWPATLEPANYTINLTGTDWLGRTMSFPLRVEVTTEYRVGGRPLAMDELLP
                     TDVGLEARVLLPVGIAPGEVDIRVNGEAGYFAWGQGADTREWIGMTTRALPAGEVTLE
                     TQVREVSTGTFRIRTQTEFGLDAIYFYPSPWSGVGPAYFTYQLSFLEGNAPRRAEVSV
                     FSISGRKVTTLVGTTTVGRNTLEWNGTDQKGDPIANGVYLYKLNIEDADGHQHATIDR
                     LVVHR"
     gene            6236..6502
                     /locus_tag="VF720_10115"
     CDS             6236..6502
                     /locus_tag="VF720_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX7879755.1"
                     /translation="MNKADLIEYVANDCGLTKKDAAAAVESTLTNIKKGVKKGNVQII
                     GFGSFAVTRRKARMGRNPQTGESIKIAASKSVRFKPGKEFKDKL"
     gene            complement(6689..8674)
                     /locus_tag="VF720_10120"
     CDS             complement(6689..8674)
                     /locus_tag="VF720_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX7879756.1"
                     /translation="MQPFLTVSRSDRRPATAPPPLFGAFPLFPLVLALVLGAFSSAPA
                     DAGPVDGRPKILLHVMAPALKNPCGRAQLADCRNAVTAGSVGAPAYVYLLGARGSLPS
                     ISAVECALFYQDGAPSAISDRTGIDIFGWTRCTALEFPSPGPRVWPAPGGGNLIVHDA
                     VQACQTGDVAVMGYFYLSAYTADALHLVERPVTGIAGVTECGAIETPLSPSDLGSVKF
                     ATGGGVEGCNPCLGPCPDAPPFPPARCVLVPPTSTAFPTVAVGDGNNHRKIILRNEGG
                     QPMTGRLQLDSPHFRVWAAQNDYLVPPYESREFWITFQPEAVGDHTGHLTIGSGCPGI
                     TFTGTAIEYTDCHIGPGTEFSETAIGDYRENVLSVWNAGPARVAGDLFLDQCTGDFAI
                     VGDPHFDIGPHETAERRIRFAPTRVGSQSCRVISTSPLCFGATVSGIGYAGEARPPEC
                     VVWETPVDFGEVMVGGTGEAHFHVRNAGGGSLYLQLFPNCPEFLADPGFPSNMRLSAG
                     QDGVVRLQFRPTDAGPITCELNMADCAPVMLTGVGRLPGPGLLTPDAIDFGLVAVGQP
                     VTQSFTVTNSGGQIIRGAITSSRPEFRLVGSGEYDLPPGASASFAVRFEPVEPGAISG
                     RLDAGASSSILPLTAVAELPTPVRTTSWSAIKSRFGN"
     gene            8819..>9455
                     /locus_tag="VF720_10125"
     CDS             8819..>9455
                     /locus_tag="VF720_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX7879757.1"
                     /translation="MRPDRLTVKAQEALERARALAASHSQQAIDPLHLFLALVQEEEG
                     AIGAVLDKLGVDADRLARDLEAGVERLARVSGAVAGQFVSPDLAKLLDKAEQEATTLK
                     DEYVSTDHLLLAMTDRVAGSAIQKIVASAGITRDGLLKALVDVRGGQRVTDPGAEDKY
                     QALEKYGIDLTERARAGKLDPVIGRDSEIRRVIQVLSRRTKNNPVLIGEPGV"
BASE COUNT         1724 a         3156 c         3159 g         1416 t
ORIGIN      
        1 ccaggaagac gccgtagtgg agcgcgtgct gcggcaggaa gagccggggg gaaccctcgg
       61 cccccacgcc cgtccgtacc caggcgagca tctcgtcgcg gtaggtggcg ccgttccaga
      121 tcaccgtctc ggtcccggcg ggattccatc gcgtgttcag gatgacggcc accgagaggg
      181 cggtgctcca cgccaccatg aggcccagcg ccgaggacag ccggccggcg ttgatgcgat
      241 ccaggaagac gaggatggcg gggagcgcgg tgagcacggg gaagaggccg ggctgtccga
      301 tccggatccc caaccacacc gccgcgggcg ccgccacgag caggaaagcg agggccgcca
      361 tgtcgcgcgg aaagaagcgc ggacgggcaa ccgtggccgc ggtggaggcg gctgggatcg
      421 cgctcatgcg aggtacgcca ccatgaagcc gatctgcgtg acgaacatca tccagtacat
      481 gtgcgtccag gaagggtggt tctcggtgga cgccagctcc tcggggcggc gcaggatcag
      541 ccagcagaca tagcctcccc agagcgccag gcccacgccg aggagcgtca ggaatatcgg
      601 gttgccggtc agcaccttca ggtagacgcc caacggcagc agaaggaacg ggagcacgaa
      661 cgaaggggag atcatccagg cgctcttcgt cacgccgtac tggatgggca gcgtgcgaca
      721 gccgtcggcc ttgtcacctt cgatgtcgga gtagtccttg gtggtggcgg cgcccaggag
      781 gaacaggccg aagatcgagc cgatgaacca gggctccgcc ccgaagatgc ttttcacggt
      841 ggaccagccg gccaccttga gcagcacgcc gcgcgggatg gcgatcgtga tattggccca
      901 gatcccttcc cgcttcgtgc ggagcggcgg cgccgaatag gcccaggtaa agatcgtggc
      961 ggccagcgcc agcgcaaagc attgccagtt gacgagggcg gccaggagga gggccacgcc
     1021 gaaggtgatc caggtgaacc agccggcctc gcggatcgac attcgccccg acgggatcgg
     1081 gcgcccgggc ttgttcaccc ggtcgttcgt gaggtcgtgg atctggttca ggccgttcga
     1141 ggcggcgttc aggacgccgg ccatcagggt gcccaatccg atgttcacga cgtgatgcca
     1201 cgcgaacgtt tcgtggggat gggcgccgaa ggcggtgacg ccgccggaaa ggatgccgag
     1261 ggccggcgcg acgagcgtga agggacgggc aaactcccag tacagtgccg cccgcttcga
     1321 gagtgacaat gtgatggaag cccccgaaat tgccgataac ctaacttgcg tcagatcaac
     1381 gactaaagct accatcccga atgtagaacg tcaatctcgt cccgatggcc ccgtccgggc
     1441 ggctttcccg ccgcaacgag agcgggtcgg aagggacggt gtcgtcatta ccgaggaact
     1501 ggttcagggg tattgtgttc gttgcgcggg ccgaggctgc gcacggccac cgatctggac
     1561 atccccttcc agaccccgct catggagagt acatgaccga ttgcgcgcgt cgtccgacat
     1621 tcccgcccgc ccgcatgatc cgaacgatcg aaatcgccgc cctgatactc ctggccagct
     1681 tctcgtgggt tgccccggtt gcccgggccg aggaggccac gccgctgaaa ctcgtggcgg
     1741 cggatgccac gggagtgact ctggaataca cggcgcccga accgagattc actcccaccg
     1801 aaaccccgtt cggaaatttt gaccggatcg agatggacgg gcacggcacg agcggcttcc
     1861 tcggccagcc cagcctgccg gaaacgggaa ccgggatcgg cgttcccgag ggcgccgaac
     1921 cgcgccttac gttcgagatc ctcgagtcga tcgagctcac cggacatcgt ccgctcccga
     1981 tgggggacgc gccgacggcg cccggatcga cgccgacccg ggtggaccgt tcggccttcg
     2041 acgccgcggc ctacgagccc gggaacgaac ccgtccgcga accgatcgcg tacgccgagc
     2101 tcggccagct ccggcaccag cgaatcggcc aggtgtctgt ccgtccgctc cgcctccatg
     2161 acggcgacca gctcacctgg atccggcgcg cgcgcatccg cgtcgacttc cgcatgccga
     2221 gccgcgccgc caatgcggcc acgcttccgg tgggagtcga gccggcctgg gaagaggcgt
     2281 accggggggc catgatcaac tacgagtcgg cccgctcgtt ccgttcccgg cccatgccga
     2341 atccggccgg ccgggggggc gatcgcagcc tcctcgaatc gaccgacgcg gcgatcgacc
     2401 tggcccgcgc catcgacggc aacccggaga tgcgcctgtt cgtggcgaag acgggcctct
     2461 actacctgtc gttcgccgac ctttcggcga aggggtggcc cgacaacgtg gccatcggcc
     2521 aggtgtcgtt cttcgaaaag tcgtacaacg cggccgaccc cgccaacccc ctgatccgcg
     2581 acatccccgt cacgatcgaa gagggaacga ccggcacgcc cggcatcttc gacggcgacg
     2641 acacgatcta cctctacgtc atgaacttcg ccgaccggac gcaggccacc ccgcagttca
     2701 cccgcttcag caacctgcag gcctactggc tctcctggcg cgccgccggc ggcagccgca
     2761 tgacgagcga tccggggtgg ttcgaatcgg cctccgtaac gactccctcg agctacgtca
     2821 tggaaacgca ctacgagcag aatccctact acctcaccga ccgggtggaa gaggagagct
     2881 accaggggaa cgagacgttc catccgaaga tcgaaggcta tttctggttg gaagggatcg
     2941 tctatcgcga cagcctccag ttccagacgc cggggcgtga cgccacccgc ccgtatcgct
     3001 tccgcgcgcg atggcaggga ctgtttgccg gcaaccacca tgccaaggtg tggcagaaga
     3061 ccggctgcgg ggccaacgcg ccggacacgc tgatcggtcg aacgcccccc tacatgaata
     3121 tcgtgaatcg ggggggattc tgttccggct gcgcctacat catggatacc ggttaccggg
     3181 tgccgggcgc gcggctcaac gacggctgca acgagatccg gctccagggg aacggcgtgg
     3241 ccaacggcga cacgaccagc gaggggagcg gcgcctactt cgactggttc gaggtcgatt
     3301 acccgcgcag gtacgtggcg tccgggggga cgctccgctt cacctcgtcg aacgccacgg
     3361 gcgatgtgga gttcgtcatc tccgacctgc gcgagagcga catccgcgtg ttcgacatca
     3421 ccgactcgct ggcgccccgg ctggtggcta tcgatccctc gaagatcgtc cagacccagg
     3481 gggcgccgaa ccggcgctgg caggccacga tgagggtccg tgtgaccgga ggccagcgca
     3541 cctacgtgct ttgggaatac agccagattc cgaacgtgtc gacatcggtg gccaacggcc
     3601 ggatgccgaa agaccagggg acgaccaacc tggccctgga cacccccgac cagctcacca
     3661 ccgacacggg cgccaacgtc ctggtcatct gccatccgga tttccgcacc acgttcgaac
     3721 cctggaaggc gctgcgcgag ggacagggaa accggctccg cttcgtgtca ccgcaggaca
     3781 tctacgacca gttttcgggc ggcgtgaaga cgccttatgc catccggctc tggttgcgct
     3841 acgtgtacca gaactggacc gtggccccgg acgacgtggt gctcatcggc gacgcctccg
     3901 aggactaccg ctccgacgtg accggcaacc cgaaattcaa gagcgacccc gactgggtgc
     3961 cgaccatgat gatctacgga ccggtgccca acggcgccgg ccgccgcgag aagatcggca
     4021 ccgaccactg gtacatcggc tccctcggcc ccgggcaggg ggagttcgac atgctccccg
     4081 agatgtacat cggacggatc tcggtgaaca gcaccaccga cgcccagatc atggttgaca
     4141 agctggtggc ctacgacaac tacaccgcca acgatacctg gcgaaaccgg gggctcatcg
     4201 tcagcgacga ccagttttcg agccccctgg ccggtttcaa tgactatcgc taccaaccga
     4261 ccgagctcat cttcgaggaa gtgggggact cgctggccca catctacaac gggctttcct
     4321 gctacaacgg cttcgaggcc acccactggc gcctggacga ctacctcacg gcgctcaacc
     4381 gccccaagcc tccctgcccg ccgaacaatc ccacctgcca ggggaacgcc ctggcggccc
     4441 aggcggaagc gcggcggacc gccaccccgg catgggtcga ggaatcgagc aaagggcacc
     4501 tgttccagat ctattccggc catgccaacg cggcggtcat ggccaccgag cttttcgtgg
     4561 agttccgtgg ggatcgcctc tcgccggacg cgcggatcac cgagcagctt accaactacg
     4621 gcaagccgtt catcttcttc ggcgtggcct gccatctcaa cgaattcgag tcgtacaacg
     4681 aggggaacac caagatgtgc ctctcggaat cgatgctctt cctcgccaac cggggggcca
     4741 tcgcctcgat tgcctccacc ggtttcgagt ggctctactc caacgaagga gtggagctct
     4801 acctcggcaa ggtcatgttc gccgagcatc cgcgcgaccc cctcaccggc cggccccggc
     4861 gcatcctggc ggaatcgatg tatgccgccc tgatccggct catcaccgag cgccccaacg
     4921 acggattcct gtacaacgac acgatccgca gctacggcct tctcggcgat ccgaccatgc
     4981 ggatcgacat ggcgccgccc atcctggcgg tgacggtcga tggggcgccc gtcgagccga
     5041 acacggcggt ccgggcggcg ccgggcgccg acgccgtgac cgtccaggtc aaggtggccg
     5101 acgacgtgga cgacaaggcg gacagcttcg ccatcacggc cggcgagacg gcggtgccat
     5161 cctccgagtg gacctccacg ccgttccaga ccagcgacct cggcgcctgc cacggcgtcc
     5221 ggatcgactg gccggccacg ctcgaaccgg ccaactacac catcaacctg accggcaccg
     5281 actggctggg gcgcacgatg tcgttcccgc tccgggtgga ggtcacgacc gaataccggg
     5341 tgggggggcg accgctcgcc atggacgagc tcctcccgac cgacgtcggg cttgaggcgc
     5401 gggtgctcct cccggtgggg attgcgccgg gcgaggtgga catccgggtg aacggcgagg
     5461 ccggctactt cgcctgggga cagggcgccg acacccgcga gtggatcggc atgacgacgc
     5521 gggcgctccc ggcgggagag gtcacgctcg aaacgcaggt gcgcgaggtg tccaccggca
     5581 ccttccgcat tcgcacacag accgagttcg ggctcgacgc catttatttc tatccgagtc
     5641 cctggagcgg agttggcccg gcctacttca cgtaccagtt gagcttcctc gaggggaacg
     5701 cgccgcgtcg cgccgaggtc agcgtcttct cgatctccgg ccgcaaggtg acgacgctcg
     5761 tcggcacgac caccgtgggg cggaacacgc tggagtggaa cggcaccgac cagaagggcg
     5821 atccgattgc caacggcgtc tacctctaca agctcaacat cgaggatgcc gacggccacc
     5881 agcacgccac gatcgatcgg ctggtcgtgc atcggtgagg ttggtgggca tcagtccgga
     5941 tgacggccgg gcgtgggccg ggtctgcgcc gggatgtccg ccgagcctga gtccagagga
     6001 ccgcgtccat cacggattgc ccgaatcgcg cggcgcgagt cccgcccggg tggctcgatc
     6061 cccgagacac gtcgacggaa atccgcatca tttcaaggaa tttcatcttt ctttccttga
     6121 cggttcgtcc gcacggtgat aaatcctgcg ttatcacggt gcgtgagttg accgcgcgca
     6181 tcacgtcgag ccccgaccgg ggagcggcgt tcggggtcat accggaggaa cgtccatgaa
     6241 caaagcggac ctcatcgaat acgttgccaa tgactgtggc ctgaccaaga aggacgcggc
     6301 tgccgccgtc gaatctaccc tcacgaacat caagaagggg gtcaagaagg gcaacgtcca
     6361 gatcatcggc ttcggttcgt ttgcggtgac gcgtcgcaag gctcgcatgg gccggaaccc
     6421 gcagaccggc gaatcgatca agatcgccgc ctccaagtcg gtccggttca agcctggcaa
     6481 ggagttcaag gacaagctct agatcccgcc acagacgtca aagcctgcaa ttcgcgcggg
     6541 cccgatgccg gaacggcgtc gggcccgctg cgttggggtg cgcggagcgg gggaggggaa
     6601 ctctgcctga accgagttgg cggacctgta atgacactag gcccgactct gcctgaaccg
     6661 agtcggcgga cctgcttgtc gcacaggttc aatttccgaa gcgggacttg atcgcgctcc
     6721 agctcgtcgt gcgaacgggt gtcggcagtt cggccacggc ggtcaacggc aggatggagg
     6781 aactggcccc cgcgtccagc cggccgctga tggcccccgg ctcgaccggc tcgaaacgga
     6841 ccgcgaagct ggccgaggcg cccggcggca gatcgtactc gccggatccg accaggcgga
     6901 attccggccg actgctggtg atggcgccgc ggatgatctg gccccctgaa ttcgtgacgg
     6961 tgaacgactg cgtgaccggt tggccgacgg ccacgaggcc gaagtcgatg gcatccgggg
     7021 tcagaaggcc cggccccggc aggcgtccga cgccggtcag catgaccgga gcgcagtcgg
     7081 ccatgttcaa ctcgcaggtg atcggcccgg cgtcggtggg ccggaactgc agccgcacga
     7141 cgccgtcctg gccagcgctc aggcgcatgt tcgacgggaa cccggggtcg gccaggaact
     7201 cagggcaatt cgggaagagc tggaggtaga ggcttccacc cccggcattc cggacgtgga
     7261 agtgcgcctc cccggtccct cccaccatga cctcgccgaa atccacgggc gtttcccaca
     7321 ccacgcactc gggtggcctc gcctcgcccg cataaccgat gccgctcacc gttgcgccga
     7381 agcagagcgg gctggtcgaa atcacgcggc acgactggct gccgacccgc gtcggggcaa
     7441 accggatgcg tcgctcggcc gtttcgtggg gaccgatatc gaagtgggga tcaccgacga
     7501 tggcgaagtc cccggtacac tgatcgagga acaggtcgcc ggcgacccgc gccggaccgg
     7561 cattccagac ggagaggacg ttttcccggt agtcgccgat cgccgtttcc gagaattccg
     7621 tgcccggccc gatgtgacag tcggtgtact cgatggccgt gccggtgaag gtgatgcccg
     7681 ggcagccgga cccgatcgtg aggtggcccg tatggtcgcc gaccgcctcg ggctggaacg
     7741 tgatccagaa ctcgcgcgat tcgtagggag ggaccaggta gtcgttctgg gcggcccaga
     7801 cgcggaagtg gggcgagtcg agttgcaggc ggcccgtcat tggctggcca ccctcattgc
     7861 gcaggatgat cttgcggtga ttgttgccgt cgccgaccgc cacggtggga aaggcggtgc
     7921 tcgtcggcgg caccaggacg cagcgtgccg gtggaaacgg cggtgcatcg gggcaggggc
     7981 cgaggcacgg attgcacccc tcgacgcccc ccccggtggc aaatttcacg gagccgaggt
     8041 cggagggaga gagcggcgtt tcgatggccc cgcactcggt aactcccgcg atccccgtga
     8101 cgggacgttc cacaaggtgg agcgcatccg cggtataggc cgacaggtag aagtatccca
     8161 tgacggccac gtcaccggtc tgacaggcct gcaccgcgtc gtggacgatc aggttgccac
     8221 cgcccggggc cggccagacc cgggggcccg ggctcgggaa ttcgagcgcc gtgcagcgcg
     8281 tccagccgaa gatgtcgatc ccggtccggt ccgagatggc cgagggcgcg ccgtcctggt
     8341 agaagagcgc gcactcgacg gcgctgatcg atggcagtga gccccgggcg ccgagaaggt
     8401 agacatacgc cggcgccccc acgctgccgg ccgtcaccgc gttgcggcaa tccgcaagct
     8461 gcgcccggcc gcagggattc ttgagcgccg gtgccatgac gtgcaggagg atcttcggcc
     8521 gaccatccac ggggccggcg tcggccggcg cggaggaaaa cgccccgagg acgagcgcga
     8581 gcaccagcgg gaacagcgga aaggcaccga agagaggtgg cggggccgtt gccgggcgcc
     8641 ggtcggatcg tgacaccgtg aggaacggtt gcatgagaat cctccggatc gcgcgagtca
     8701 tccagttgac gaccccttga ggatatgatg gacaatcctc actgtccaac gggttctccc
     8761 gggatcggac gatggatggg cgctggcccg tccgatacaa aatcaatatg aggtccccat
     8821 gagacccgac cgactcaccg tgaaagccca ggaggcgctc gaacgcgccc gggcgctggc
     8881 cgcttcgcat agccagcagg ccatcgaccc gcttcacctc ttcctcgcgc tggtccagga
     8941 ggaggagggg gccatcggcg ccgtactcga caagctcggc gtggatgccg atcggctggc
     9001 ccgcgacctc gaggccggcg tggagcgcct ggcccgggtg tcgggtgccg tggccggcca
     9061 gttcgtctcg ccggacctgg ccaagctgct cgacaaggcc gagcaggaag ccacgaccct
     9121 gaaggacgaa tacgtctcca ccgatcatct tctgcttgcc atgaccgacc gggtggccgg
     9181 aagcgccatc cagaaaatcg tggcctcggc cggcatcacg cgcgacggcc tgctcaaggc
     9241 gctggtcgat gtccgtggcg gccagcgcgt caccgacccg ggcgccgagg acaagtacca
     9301 ggcgctcgag aagtacggga tcgacctgac cgagcgggcc agggcgggaa agctcgaccc
     9361 ggtgatcggc cgcgactcgg agatccggcg cgtcatccag gtgctcagcc gccgtaccaa
     9421 gaacaacccg gtgctcatcg gcgagcccgg cgtcg
//
LOCUS       DASVOD010000445         9340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_447722, whole genome shotgun sequence.
ACCESSION   DASVOD010000445 DASVOD010000000
VERSION     DASVOD010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447722"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF720_10130"
     CDS             <1..210
                     /locus_tag="VF720_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016541786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879758.1"
                     /translation="LWTRVSRTLLFWSAFILTRPLGAVIGDFLDKPVAKGGLALDRFA
                     ASGVLVAFLVACLLLLPQKPARSAH"
     gene            302..1843
                     /locus_tag="VF720_10135"
     CDS             302..1843
                     /locus_tag="VF720_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX7879759.1"
                     /translation="MSVGLIAAAIMTAPPAAGWDQPGYSLEWQADTGYGLATDGQQQI
                     IALGQPPYAYGPEGQLLGRFGENLVAVPPLWAVVAMSPAGNTHLFARNIWTYGQAMVV
                     ELDRHGRLVRQVPLPPQLAVANGNYDFRGIAVDAQGRLYLSAYEAGEIHVFGTDGTQV
                     ATWGSAGSGSGQLMNPSRLMFDADGTLFVADRGNGRIQRLTPDGNFLPPLEASGASFK
                     DFGFDRAGNIYCLSAYGGVRVYDRQFNELGRWNDRGPGKTLDGARLLVVDGNDNVYVA
                     VEYGVIVKFDSRLVIPPAGSTYSPKLVLSALPAAGMSHPCRTAGEQSLESAQTTGELA
                     DRDPRPYYFVCLLVARGNGSYLAGVQCGLSYHDHPSDQDPGRGLEIFDWHLCADLEFP
                     TPAGTPSGAPAWPGAGSGNLITWDPIFNCQKLDFAVAGYFYVGAYSATTFSLTPRPVD
                     MAAKVAYCATSGEFPLVQADLGFVNFSPGGTAAGCNPVLSACTETTPVKPTTWSRIKA
                     ISTRQ"
     gene            complement(1983..2819)
                     /locus_tag="VF720_10140"
     CDS             complement(1983..2819)
                     /locus_tag="VF720_10140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002654415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7879760.1"
                     /translation="MSFSMRRGGNMVGGRFLIAIILALVSVAGYYMSSQKNPITGENQ
                     HVAMSPEQEVALGLQAAPEMAQQYGGLYPDEEAQALVDRVGNKVVAGSPAAQSGYPFE
                     FHLLADPNTINAFALPGGQVFITAALLNKLDTEGELAGVLGHEVGHVIGRHGAEHMAK
                     AQLTQGLTGAAVIATYDPNDPSTRNTAAVTAMIGQLINLKYGRDDELESDKLGVKFAS
                     DAGYDPRSMLRVMTVLADASSGQAPPEFMSSHPDPGNRSERIEQAIQEMFPNGVPEGL
                     VK"
     gene            complement(2861..3652)
                     /locus_tag="VF720_10145"
     CDS             complement(2861..3652)
                     /locus_tag="VF720_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX7879761.1"
                     /translation="MARMPLTYAQYLKLDELLKLQEARSKPAEHDELLFIVIHQVYEL
                     WFKLLLHELDKTGRDFEQGDLYGAIHTLKRSRTIMKTLVGQLDILETMTPMSFTSFRD
                     RLETSSGFQSSQFRELEYLLGFKRAEMLKFCDRESGAYAAAGQRLNSPSLIDQFYKFL
                     ATRGANVPASLAARPVADPAEPSEELQEEILRLYKTKPELTILFELMTDFDEGMQEWR
                     YRHVKIVERTIGNKQGTGGSMGVEFLKKSLFRPIFADLWAIRHRL"
     gene            3939..5738
                     /locus_tag="VF720_10150"
     CDS             3939..5738
                     /locus_tag="VF720_10150"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEX7879762.1"
                     /translation="MIRLRTTFFCTAALVILHASVAVSWSAERTIPRLDSGARLPRVA
                     DMREGDTYVRGRTTIHVVAPDDPRLLNAIEAMKSARRLGHPRAATDLQVDLLGRGYDR
                     DGRLREPVRTGSATPGVTAALGWAPFSGTFQIDEAAHLLHRAMIGPRFEEMQAVTTAG
                     LPSTLTSLLTPKALPPPPGAWVNEAPPDWEHITPAEEAILAQNYQDRQEIIRHWWVER
                     IVNSQTVNMTDAMVLFWHDHFATGADAVWYPMAIYGQIELIHQHALGNFKQLATDMCT
                     DPAMLIWLNGNYNTKWDPNENFARELLELFTMGEGSGYTQADVTAAARACTGWYTDGR
                     DVYFVPNWFDNTNKTFLGQTGNWEMDDLVNIICQQPATAEYLCRKIYKYFLDDDPDPV
                     DVAALATTLRNNNYAMAPMLSQMLGSNHFFDPTFRGSLIKDCIDLYAGQVRALHASGF
                     DPVTSANDYERYWVDWQSYDLGQMLTMPPNVAGWPGHHNWINTATLPLRKEYSAMILT
                     GNMWGWPLGFSVNSLTEGQRFSNPNNAGQLVDDAALLLLGTTPTTAVRNGMQNALLQG
                     MNPASWSIGMAGAGNRVRDMFRYTMRLPDYQLK"
     gene            5748..7271
                     /locus_tag="VF720_10155"
     CDS             5748..7271
                     /locus_tag="VF720_10155"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX7879763.1"
                     /translation="MKRRQFMTRMSGILSGTLLGKSVWWPVTREALAGTASNNQRILV
                     MIVLQGGNDGLNTVIPVADPLYYSKRPNLAVPQAQTLGIAPGAALHPQLAPLMDVWNT
                     GRMAIVRDVGYPDMNLSHFRATDIMFSGSSSTSVIQTGWLGRWLEAGNPTFPTILPPD
                     PMALQQGLSAGLLLQGDRGVTGVVVNDPSSFHWLVTSNYNGPFNDATPATRGGSELGF
                     VRSIDRASFEYANAIEAAADAGANHVAYPEGNELADQLSVVARLINGGMGTPVYVTGL
                     DGFDTHAGQLGAHPALLSNLAQASAAFIADLRAMGRFQDVLIMTVSEFGRRPEENGTD
                     GTDHGTAAAWFLISDGIDGGLYGAPPNLADLDDNGNTAMQYDYRSVYSSVLQGWFGTD
                     PALTSTILQGSFPMLPLMTQVGVPEPGGAPRTRLLPPTPNPGRGPRTIRFELARSARG
                     RVSVFDVGGRQVAQVAEGNWPAGSHEVRWDPAGIAPGLYFISLDADGKRQTQKLMQQ"
     gene            7333..8118
                     /locus_tag="VF720_10160"
     CDS             7333..8118
                     /locus_tag="VF720_10160"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX7879764.1"
                     /translation="MPPARGREMPGQLRICAITDPGLKRSGNEDSFEWWPRNPEEADQ
                     RGILLLVCDGMGGAQAGERASHLAVETIVRVWREAPPGDPVSAIRTAFEAANTAVNDE
                     SRADPSRRGMGTTGTAVVIIGSDLVVAHVGDSRAYLADGASLTQLTADHSLVAHLVER
                     GQITPEEARVDPRRNVVTRSIGVTPQIEVDVTEHTGALTPGSTVLLCSDGLHGVVSDE
                     EIGEHLALPDVADVGARLIDHANRMGGPDNITALLARRIANGS"
     gene            8115..>9340
                     /locus_tag="VF720_10165"
     CDS             8115..>9340
                     /locus_tag="VF720_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7879765.1"
                     /translation="MRACGIGALARSLAALSITLVALAGCSGDDGPVTGNVRVTLRNA
                     SLGIDVDTGEIDTLWITLLDMEGEPVDSTLVLPAPPLSGGTRTFDLEAPIAAGLTILV
                     KATGERPVAGAPPATYVRGILRRGLSAPFDMTAGGMAPVDVELTSFVTQFHDVAFMND
                     GARHRVSWQPLAGAVRWRLSIREGALPARDTVLVDTSFTATGLPVTYQVQAIDADTAE
                     GAPGDRLMPGLLLPGPPVNLRLIPRTGERMEVRWNRGTGFVRDWEIERRAEGGNFLPL
                     ISLDVDTLLFSDSTLVDGRRWEYRARSRNANGVSAWTTTVAAFTPLNVPDGLVLTPND
                     LEFGLGWTERSLSEEGFVLERATGNGTFIPLATLPVNASGYTDAPLAERTRYRYRVQA
                     FRGEIRSAYSTVVEGTT"
BASE COUNT         1507 a         3174 c         3100 g         1559 t
ORIGIN      
        1 ctctggacgc gcgtgtcgcg cacgctgctg ttctggtcgg ctttcatcct cacgcgcccg
       61 ctcggcgccg tcatcgggga cttccttgac aagccggtgg cgaaaggggg gctggcgctg
      121 gaccgatttg cggcgtccgg ggtgctcgtg gcgttcctgg tggcctgcct gctgctgctg
      181 ccgcagaaac cggcgcgatc ggctcactga gtgccggtgg gggactgacc cgcacgggaa
      241 gtggagtatc atggtctcat cctcaccccg gagagtgacc atgcgccgat gtgtgccagg
      301 attgagcgtc ggactgattg cggcggccat catgaccgcc ccgccggccg ccggatggga
      361 ccagccggga tattcactcg aatggcaggc cgatacgggg tacggactcg ccaccgatgg
      421 gcagcagcag atcatcgccc tcggccagcc gccgtatgcc tacggcccgg aagggcagct
      481 cctcggccgt tttggcgaga acctggttgc ggtgccgccc ctctgggcgg tcgttgccat
      541 gtcgccggcc ggaaacaccc acctgttcgc gcggaacatc tggacttatg ggcaggcgat
      601 ggtcgtcgag ctcgatcgcc acggacgact ggtccggcag gtgccgcttc ctccccaact
      661 ggccgtggcc aatggaaact atgacttccg tggcatcgcg gtggacgcgc agggacgcct
      721 ctacctgtcg gcctacgaag ccggcgagat tcacgtcttc gggaccgacg gcacccaggt
      781 ggccacgtgg gggtcggccg gcagcggttc cggtcagctg atgaatccct cccgcctgat
      841 gttcgacgcc gatggcacgc tgttcgttgc ggatcggggg aacggccgca tccagcgcct
      901 cactcccgac ggtaattttc tcccgccgct ggaagcgagt ggcgcgtcgt tcaaggactt
      961 cggattcgat cgcgccggga atatctattg cctttccgcc tacggcggcg tccgggtgta
     1021 cgaccggcag ttcaatgagt tggggcgctg gaatgaccgg ggcccgggga agaccctgga
     1081 cggggccagg ctcctcgtgg tggatggcaa cgacaacgtg tatgtcgccg tggagtacgg
     1141 tgtcatcgtc aagttcgatt cgcggctcgt catcccgccg gccggttcga cctactcacc
     1201 caagctggtg ctctcggctc tccccgctgc ggggatgtcg catccgtgcc ggacggccgg
     1261 tgagcagtcg ctcgaatcgg cgcaaacgac gggcgagctg gccgaccgcg acccgcgtcc
     1321 ctactacttc gtctgcctgc tcgtggcccg cggaaatgga tcgtacctgg cgggggtgca
     1381 gtgcggcctg tcgtaccacg atcatccctc cgatcaggat ccgggtcggg gactcgagat
     1441 cttcgactgg catctttgcg ccgacctgga gtttccgaca cccgccggca ctccctccgg
     1501 cgcaccggcc tggcccggag ccgggtcggg gaacctgatc acgtgggatc ccatcttcaa
     1561 ttgccagaag ctcgactttg ccgttgccgg ctatttctac gttggagcct actcggccac
     1621 cacgttcagc ctcacgcccc gtcccgtgga catggcggcc aaggtggcct actgcgccac
     1681 gtccggcgag tttccgctcg tccaggccga cctgggcttc gtcaacttct ctcccggcgg
     1741 tacggccgcc ggctgcaatc cggtcctgtc ggcatgcacg gaaaccacgc cggtcaagcc
     1801 gaccacctgg agccggatca aggcgatttc cacccggcag tgacggaatc agccaactgt
     1861 ggctatccac agttggctga cctgattacg ggttcccatc gccggcgaca ctcaccccga
     1921 cgcctgcgga actcattccg aatcagtcct gcgacatcgg cattcaccgc gccgacatca
     1981 ggctacttca ccaacccctc cggcaccccg ttcggaaaca tctcctggat ggcctgctcg
     2041 attctttcgg agcgattgcc cggatcgggg tgcgagctca tgaattccgg cggggcctgg
     2101 ccgctggatg cgtcggccag gaccgtcatg acgcggagca tcgagcgggg gtcgtagccg
     2161 gcgtcggagg caaatttcac gccgagcttg tcggattcga gctcgtcatc gcggccgtac
     2221 ttgaggttga tcaactggcc gatcatggcg gtcaccgcgg cggtgttgcg ggtgctgggg
     2281 tcgttgggat cgtaagtggc gatgaccgcg gcgccggtga gcccctgggt gagctgggcc
     2341 ttggccatgt gctcggcgcc gtggcggccg atgacgtgac ccacttcgtg gccgagcacg
     2401 ccggccagct ccccttcggt gtcgagcttg ttcaagagcg ccgcggtgat gaacacctgg
     2461 ccgccgggaa gcgcgaaggc gttgatggta ttggggtcgg ccaggaggtg gaattcgaag
     2521 gggtaccccg actgggcggc gggggagccg gccaccacct tgtttccgac ccggtcgacc
     2581 agcgcctggg cctcctcgtc cggataaagt ccgccgtact gctgggccat ttcgggcgcg
     2641 gcctgcaggc cgagcgccac ttcctgctcg gggctcatgg ccacatgctg gttttcgccg
     2701 gtgatggggt tcttctggga cgacatgtag taaccggcca ccgagacgag ggccaggatg
     2761 atggcgatga ggaaacgccc gccgaccatg ttgccgccac gtcgcatgct gaaactcatt
     2821 gaggacctcc tgcggggtga cgcggcggcc gtccgcgtgc ctataaacga tgccggatcg
     2881 cccagagatc ggcgaaaatc ggacgaaaaa ggctcttctt caggaattcc acccccatgg
     2941 accccccggt cccctgcttg ttgccgatcg tgcgctcgac gatcttgacg tggcggtagc
     3001 gccactcctg cattccctca tcgaagtcgg tcatgagctc gaagaggatg gtcagctcgg
     3061 gcttcgtctt gtacaggcgc aggatttcct cctgcagctc ctccgatggc tcggccggat
     3121 cggccacggg acgcgccgcc agcgaggccg gaacgttcgc gccgcgcgtg gccaggaact
     3181 tgtagaactg gtcgatcagc gaaggcgaat tcaatcgctg cccggccgcc gcgtaagcac
     3241 ccgattcgcg atcgcagaat ttcagcatct ccgcccgctt gaagccgagc aggtattcga
     3301 gctcgcggaa ctggctcgac tggaagccgg acgacgtctc gagccggtca cggaagctgg
     3361 tgaaggacat cggcgtcatc gtttccagga tgtcgagctg gccgaccagc gtcttcatga
     3421 tggtgcggct gcgcttgagc gtgtggatgg cgccgtacag gtcgccctgc tcgaaatcgc
     3481 ggccggtctt gtcgagttcg tgcagcagga gcttgaacca gagctcgtac acctggtgga
     3541 tgacgatgaa gaggagctcg tcgtgctcgg ccggcttcga acgggcctcc tggagcttca
     3601 ggagctcgtc gagtttcagg tattgcgcgt aggtgagtgg catgcgggcc atgctacgcg
     3661 acaccgcggg ggctgtcagc cccggccggg cgtccgggat gaaaaatttg ccctttcggg
     3721 cggcgcaaaa cggcatgcgc gagaggctgt gcaagccttg ttccttcccg aaggaaaacc
     3781 ggggggaatc ccccgcatgg tgcggcattt tgcttgcacc ggaaggccct gtccgtcgat
     3841 catgcccagg ccatcgggga aggcccgatc ggtgaagccc ccacggccgc agggacttgt
     3901 tccgccgacg gaccccacga ttccaagggg agcggtacat gatccgattg cgcaccacgt
     3961 tcttctgcac ggccgcgctc gtcattctgc acgcttcggt ggcggtttca tggtccgccg
     4021 agcggaccat tcccaggctc gactcgggtg cgcggctccc gcgagttgcc gacatgcgcg
     4081 agggagacac gtacgtccgc gggcgcacga cgattcacgt cgtggctccg gacgatcccc
     4141 ggctcctgaa tgccattgag gcgatgaagt cggcccggcg cctcgggcat ccccgcgcgg
     4201 ccaccgatct ccaggtcgat ctgctgggac gcggttacga ccgcgacgga cggctgcgcg
     4261 aaccggtgcg caccggatcg gccacgcccg gggtgaccgc cgcactcggc tgggcgccat
     4321 tctcggggac gttccagatc gacgaggcgg cccacctcct gcatcgcgcc atgatcgggc
     4381 cgcgcttcga ggagatgcag gcggtgacga ccgccgggct tccctcgaca ctgacgtcgc
     4441 tcctcacgcc gaaggccctg ccgccgccgc ccggcgcgtg ggtgaacgaa gcacctcccg
     4501 actgggagca catcacgccg gccgaggaag cgatcctggc gcagaactac caggaccggc
     4561 aggagatcat ccgccactgg tgggtggagc ggatcgtcaa ctcgcagacc gtgaacatga
     4621 ccgacgcgat ggtgctcttc tggcacgacc attttgccac cggggccgac gccgtgtggt
     4681 acccgatggc catctacggg cagatcgaac tgattcacca gcacgcgctc ggcaatttca
     4741 agcagcttgc caccgacatg tgcaccgacc cggccatgct gatctggctg aacggcaact
     4801 acaacaccaa gtgggatccg aacgagaact ttgcgcgcga gctcctcgag ctcttcacga
     4861 tgggggaggg ctccggctac acgcaggccg acgtgaccgc ggcggccagg gcctgcaccg
     4921 gctggtacac cgacggccgc gacgtgtact tcgtgccgaa ctggttcgac aacaccaaca
     4981 agacgttcct cggccagacc ggcaactggg agatggacga cctggtcaat atcatctgcc
     5041 agcagccggc caccgccgag tacctctgcc ggaagatcta caagtacttc ctggatgacg
     5101 accccgatcc cgtcgacgtg gcggcgctgg ccaccacgct tcgcaacaac aactacgcga
     5161 tggcgccgat gctctcgcag atgctcggca gcaatcactt ctttgacccg acgttccgcg
     5221 gcagcctcat caaggactgc atcgacctgt atgccggcca ggtgcgggcg ctccacgcct
     5281 cgggcttcga ccccgtgacc agcgccaacg actacgagcg ctactgggtg gactggcaga
     5341 gctacgacct ggggcagatg ctgacgatgc cccccaacgt ggccggctgg ccgggacacc
     5401 acaactggat caacacggcc acgctcccgc tccgcaagga gtacagcgcg atgatcctga
     5461 ccggaaacat gtggggttgg ccgctcggct tctcggtgaa ttcgctgacc gaggggcagc
     5521 gtttctcgaa ccccaacaac gccgggcagc tggtggatga cgccgcgctg ctcctcctgg
     5581 gcaccacccc gaccacggcc gtccggaacg ggatgcagaa cgcgttgctc caggggatga
     5641 accccgcctc ctggtcgatc ggcatggccg gtgcggggaa ccgcgtccgc gacatgttcc
     5701 gttacacgat gcgacttccc gactaccagc tgaagtgagg tgacccgatg aagcgacgcc
     5761 agttcatgac ccgcatgtcg ggaatcctgt cggggacgct gctcggcaag tcggtctggt
     5821 ggccggtgac gcgcgaagcg ctggccggca ccgcctccaa caatcagcgg atcctggtga
     5881 tgatcgtcct gcaggggggg aacgacggtt tgaacacggt catcccggtg gccgatccgc
     5941 tctactactc caagcggccg aacctggccg tgccgcaggc gcagacgctc ggcatcgcgc
     6001 cgggggccgc gctccatccg cagctcgcgc ccctcatgga cgtgtggaac accggccgga
     6061 tggccatcgt gcgggatgtc ggctatcccg acatgaacct ctcacacttc cgagccaccg
     6121 acatcatgtt ctccggttcg agctccacca gcgtgatcca gaccggctgg ctggggcgct
     6181 ggctcgaggc cgggaacccg acgttcccga cgatcctccc tcccgacccg atggcgctgc
     6241 agcagggact gtcggccggc ctcctcctcc agggggatcg cggcgtgacc ggggtggtcg
     6301 tcaatgaccc atccagcttc cactggctgg tgaccagcaa ctacaacggc ccgttcaacg
     6361 acgccacgcc ggccacgcgc ggcggaagcg aactcggctt cgtgcggtcg atcgaccgcg
     6421 cgtcgttcga gtacgccaat gccatcgagg cggccgccga tgccggtgcg aaccacgtgg
     6481 cgtaccccga agggaacgaa ctggccgacc agctctccgt ggtggcccgc ctgatcaatg
     6541 gcgggatggg gacacccgtg tacgtgacgg gcctcgacgg cttcgacacg cacgccggcc
     6601 agctcggcgc gcacccggcg ctcctctcga acctggccca ggcgtcggcc gccttcatcg
     6661 ccgacctccg ggcgatgggt cgcttccagg acgtgctgat catgacggtc agcgagttcg
     6721 gccgccggcc cgaagagaac ggcaccgacg gcaccgacca cggcacggcc gccgcctggt
     6781 tcctcatctc ggacgggatc gacggcgggc tgtacggagc gccccccaac ctggccgacc
     6841 tcgacgacaa cggcaacacc gccatgcagt acgactaccg gtcggtctac tcgtccgtac
     6901 tgcagggatg gttcggcacc gatccggcgc tcacctccac cattctgcaa gggagctttc
     6961 cgatgctccc cttgatgacc caggtgggcg tgcccgaacc gggcggggcg ccccggaccc
     7021 ggcttcttcc ccccacgccc aacccggggc gcggcccgcg cacgatccgg ttcgagctgg
     7081 cgcgctccgc ccgggggagg gtgtccgtct tcgacgttgg cgggcgacag gtggcgcagg
     7141 tggccgaggg gaactggccg gccggatccc acgaagtgcg gtgggacccg gcggggatcg
     7201 cccccggcct ctatttcatc tccctcgacg ccgatggaaa acgccagacg cagaagctga
     7261 tgcagcagta gcctcggtgc tatagtccct cccctaagtc ggtactggct ccgatacgag
     7321 gggccctgtg cggtgccccc cgcccgtggg agagagatgc cgggacaatt gcggatctgc
     7381 gccattactg accccggcct caagaggtcg gggaacgaag actcgttcga atggtggccg
     7441 cgcaatccgg aagaggccga ccaacgcggc atcctcctcc tcgtctgcga tggcatgggg
     7501 ggtgcccagg ccggcgaacg ggccagccac ctggctgtcg agacgatcgt ccgcgtctgg
     7561 cgcgaggcgc ctcccggcga tccggtctcg gccatccgca ccgcgttcga ggccgccaac
     7621 accgcggtga acgacgagag ccgcgccgat ccatcccgcc gcggcatggg aaccaccggc
     7681 acggccgtgg tgattatcgg ttccgacctg gtggtggccc acgtagggga cagccgggcc
     7741 tacctggccg acggcgcctc gctcacgcag ctcaccgccg accactcgct ggtggcccat
     7801 ctcgtcgagc gggggcagat cacacccgaa gaagccaggg tcgacccgcg gcgcaacgtc
     7861 gtcacccgct cgatcggcgt cacgccgcag atagaagtgg acgtgacgga acacacgggc
     7921 gccctcacgc ccggttcgac ggttctcctc tgctccgacg gccttcacgg cgtggtgagc
     7981 gacgaggaga tcggcgagca cctcgcgctg cccgacgtgg ccgacgtcgg cgcccggctc
     8041 atcgaccacg ccaaccggat gggcgggccc gacaacatca cggcgctcct ggcccggcgg
     8101 atcgccaacg gctcatgagg gcctgcggca tcggcgccct ggcccgctcg ctcgcagccc
     8161 tgtcgatcac cctcgtcgcg ctcgccggtt gttcgggcga cgacgggccg gtcaccggca
     8221 acgtccgcgt cacgcttcgg aatgcctccc tcgggatcga cgtggacacc ggagaaatcg
     8281 acacgctctg gatcaccctg ctcgacatgg agggggagcc ggtcgactcc acgctcgtgt
     8341 tgccggctcc gcccctttcg ggcgggacgc gcacgttcga cctcgaggcc cccattgccg
     8401 ccggcctgac aatcctcgtg aaggccaccg gtgagcgccc ggtggcggga gctcctccgg
     8461 ccacctacgt gcgcggcatc ctccgccggg gcctctccgc accgttcgac atgacggcgg
     8521 gcggcatggc cccggtcgac gtcgagctga cgtcgttcgt cacccagttc catgacgtgg
     8581 cgttcatgaa cgacggggcc cggcatcgtg tgtcgtggca accgctggcc ggggccgtgc
     8641 gctggcggct ctcgatcagg gaaggagccc tgccggcgcg cgacaccgtg ctggtggaca
     8701 cctcgttcac ggcaaccggc ctgccggtca cctaccaggt gcaggccatc gatgccgata
     8761 cggccgaggg agcgccgggc gaccggctca tgccggggct cctgttgccc gggcctcccg
     8821 tcaacctgcg cctcatcccg cggacggggg agcggatgga ggtgcgctgg aatcgcggca
     8881 cagggttcgt gcgcgactgg gagatcgagc ggcgggccga aggggggaac ttcctgccgc
     8941 tgatctcgct cgacgtggac acgctcctct tttcggactc cacgctcgtc gacgggcggc
     9001 gctgggaata ccgggcccgt tcgcggaatg ccaacggcgt gtcggcctgg acgacgacgg
     9061 tggccgcctt cacgccgctc aacgtgccgg acggcctcgt gctcacgccg aacgacctgg
     9121 agttcggcct gggatggacg gagcgttcac tatcggagga ggggttcgtg ctggaacgcg
     9181 ccacggggaa cgggacgttc atcccgctgg ccacgctccc ggtgaacgcg tcgggctaca
     9241 cggacgcccc gctggccgaa cggacgcgct atcgctaccg ggtgcaggcg ttccgcggcg
     9301 agatccggtc ggcgtactcc accgtggtgg aggggaccac
//
LOCUS       DASVOD010000446         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_759283, whole genome shotgun sequence.
ACCESSION   DASVOD010000446 DASVOD010000000
VERSION     DASVOD010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759283"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF720_10170"
     CDS             <1..543
                     /locus_tag="VF720_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7879766.1"
                     /translation="IASQVVTALQSISSRSINPLDSVVVTVASIHGGTAFNIIPPSVE
                     MRGTVRSFSKSVRKDVPKRMASIVKQIAGALGGRGELDYVNEDPALVNDARLAEFFRE
                     VVRDVMGPKALIETEPSMGGEDMALYQELVPGCYIFVGSSPKGTMYPHHHPKFNPDEG
                     VLPIATALMTEGARRWLAAN"
     gene            566..>1789
                     /locus_tag="VF720_10175"
     CDS             566..>1789
                     /locus_tag="VF720_10175"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7879767.1"
                     /translation="MSRLPRGAVAWAFYDFANTIFSMNVLTMYLLKWVSVDQGQPDLW
                     YSVAFSLSMILVAVTLPVLGTLADSSPRGRLSYLLAFTVAAILGTALIGVAARGAPGP
                     STALAALAAFVVANYAFQGSLVFYNALLPLVSTPATLGRISGFGVAMGYVGSIAGLLM
                     VAPFVEGKAGPISLTIPGIEPGGRAAAFLPTAFFYALFSIPLFLRIKIDAGARPIDTR
                     PVGVRLRAAINEVRAGLRDVGNYPGVRRFLVANWLFVNCVHTILIVMGKYALEVVGMP
                     DSTQIILFILATTGAVAGGLIAGREADRHGPKTVLVAVLLGWIACLLIVALIPGKLII
                     WLSGVAIGLCLGGTWTTARALLARLTPPDELGRFFGLYALSDKAGAVLGSLTWGIVVG
                     IGAPLGPWRYRLAVLV"
BASE COUNT          236 a          594 c          629 g          330 t
ORIGIN      
        1 atcgcctcgc aggtggtcac ggcgctgcag tcgatctcga gccgttcgat caacccgctc
       61 gactcggtcg tggtgacggt ggcctcgatc cacggcggca cggcgttcaa catcatcccg
      121 ccgtcggttg agatgcgcgg caccgtccgc tcgttctcga agagtgtgcg caaggatgtg
      181 ccgaagcgga tggccagcat cgtgaagcag atcgccggcg cgctgggcgg ccgcggcgag
      241 ctggattacg tgaacgagga cccggccctg gtgaacgacg cccggctggc cgagttcttc
      301 cgcgaggtgg tgcgcgacgt gatggggccg aaggcgctca tcgagaccga gccctccatg
      361 ggtggcgagg acatggcgct ctaccaggag ctggtgcccg gctgctacat cttcgtgggc
      421 tcgagcccga aggggacgat gtatccccac caccatccca agttcaaccc ggacgagggg
      481 gtgctcccca tcgccacggc gctcatgacc gaaggcgcgc gccgctggtt ggcggccaac
      541 tgagcggcgt aacccggatc gcacgatgag ccgactcccg cgcggggccg tcgcgtgggc
      601 gttctacgac ttcgccaaca cgatcttttc catgaacgtg ttgacgatgt acctgctcaa
      661 atgggtttcc gtggaccagg ggcagcccga cctctggtac agcgtggcgt tctcgctgtc
      721 gatgatcctg gtggcggtca cgctgccggt cctcggcacg ctggccgact cgagcccgcg
      781 gggccggctg tcgtacctcc tggccttcac ggtggcggcc atcctgggaa cggcgctcat
      841 cggcgtggcg gcacgcggcg cgcccgggcc ctccacggcg ctggccgcgc tggccgcctt
      901 cgtggtggcc aactacgctt tccaggggag cctcgtcttc tacaacgcgc tcctcccgtt
      961 ggtgtccact ccggccaccc tgggccggat ttccggattc ggcgtggcga tggggtatgt
     1021 cggatcgatt gcggggctcc tgatggtggc gcccttcgtg gaagggaaag ccgggccgat
     1081 ttcgctgacg atcccgggaa tcgagccggg tggccgggcg gcggcgttcc tgccgacggc
     1141 cttcttctac gcgctcttct cgattcccct cttcctgcgc atcaagatcg atgccggcgc
     1201 gcggccgatc gacacacggc cggtgggcgt ccgcctccgc gcggccatca acgaggtgcg
     1261 ggccggcttg cgcgacgtcg gaaactatcc cggtgtgcgg cgattcctcg tggccaactg
     1321 gctcttcgtg aactgcgtgc ataccatcct gatcgtgatg gggaagtacg cgctcgaagt
     1381 ggtgggcatg ccggattcca cgcagatcat cctgttcatc ctggccacga cgggcgccgt
     1441 ggcgggcggt ctgatcgccg gccgcgaggc cgaccggcat gggccgaaga ccgtgctcgt
     1501 ggccgtgctg ctcggctgga tcgcctgcct gctgatcgtg gcgctgatcc cgggcaagct
     1561 catcatctgg ctgtcgggcg tggccatcgg gctctgcctg ggcggcacct ggacgacggc
     1621 gcgagcactg ctggcgcggc tgacgccgcc cgacgaactc gggcgcttct ttggcctgta
     1681 cgcgctgtcc gacaaggctg gtgccgtgct cggttccctg acgtggggta tcgtggtcgg
     1741 gatcggcgcg ccgctcgggc cgtggcgcta ccgcctggcg gtgctcgtc
//
LOCUS       DASVOD010000447         3345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_655136, whole genome shotgun sequence.
ACCESSION   DASVOD010000447 DASVOD010000000
VERSION     DASVOD010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655136"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF720_10180"
     CDS             <1..258
                     /locus_tag="VF720_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015465190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7879768.1"
                     /translation="NAAAFDSIDFARKHLGRDRPIGTLDFSRTNVNGGAIALGHPVGA
                     TGTRLIITLLREMKRRNLALGLVTLCVGGGQGAAMVLERTA"
     gene            275..850
                     /locus_tag="VF720_10185"
     CDS             275..850
                     /locus_tag="VF720_10185"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7879769.1"
                     /translation="MPKIEKLFLKLPPRCRSKEVALQLGAMNDMTRRLFEEDLEKVTK
                     DELCWQPAPGRNTIGMLLAHLAIVETFWTQVGVERLDTYDIKHILGIGMDDDGIPVPP
                     GGGAYPDLKGKSLSHFEKLLEKSRKYVTRKWSPLSDRDMKKPVNRTRRDGSHAVTDPR
                     WILYHMVEHFAGHQGQILLIRHEYRDFRAGR"
     gene            850..3018
                     /locus_tag="VF720_10190"
     CDS             850..3018
                     /locus_tag="VF720_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX7879770.1"
                     /translation="METFRIESAPDGIAWLVVDAPGEKVNVLSSAVLGELQSRLETLA
                     ASSDVRAIGVISGKPGTFIAGADVDEIGAITSAAAGVEASRRGQAILGMLAMPGKPTL
                     AAIDGACLGGGLELALACHFRIAGNSPATRLGLPEVKLGIIPGFGGTQRLPRVIGFTP
                     ALDLAMTGRTIDASRAERLGLVDEAVPPAILRARGEAWLRRALTGERHGGRWARLKPR
                     RKPKLGVRVTGWMPVRPFIVLATDWKLKRTLHGDYPAPHEAMESVEESFHTKLDKGLA
                     HEAGLVGPLLVTPTCKNLTWLFRANNEARRPGAIRNPETGNAVPAREVRNAAVIGAGV
                     MGGGIAHLLAEQGIPVRLKDLDEAALVKGMSAAAGLVAAQRKKGRIDRRAARDRMSRI
                     APALTWDGFGSIDCVIEAVVEDLGIKQRVVGEIESVVPERCVIATNTSSLPIGGIAAG
                     ARHPDRIVGLHFFNPVHRMPLVEVIAGRASSAEAVATAHGLARRLGKTPIVVTDTPGF
                     LVNRVLTAYLAEALRMLVEGTDPRAIDKAMVHFGMPMGPFALLDQIGFDTASKASKAI
                     ETLNERYLPRASVMSRLIEAGRLGVKNGKGFYRYRKGKNIGFDKHVLGLVEHGDKSEH
                     GVEDIQARLYLPMVNEAVRCLAAGVARSPADVDLGMVLGTGFPPFRGGPLRNADIMGI
                     PAVVERLNELATRVGERLSPEPRLVQMGLSGERFYPPVSQ"
     gene            3064..3300
                     /locus_tag="VF720_10195"
     CDS             3064..3300
                     /locus_tag="VF720_10195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEX7879771.1"
                     /translation="MTKQRSMKRRPDRVTISPKFQIVIPQHIREELKLEPGRELYAVA
                     IDGQIVFVPLRPLEEYLGILQGPNDFEREKQDRF"
BASE COUNT          580 a         1122 c         1109 g          534 t
ORIGIN      
        1 aatgccgccg ccttcgactc gatcgacttc gcccggaagc acctcgggcg cgatcggccg
       61 atcggcacac tcgatttcag tcgtaccaac gtcaacggcg gcgccattgc gctcggccac
      121 ccggtcggcg ccaccggcac gcgcctcatc atcacgctgc ttcgtgaaat gaaacgtcgc
      181 aaccttgcgc tcggcctcgt cacgctctgc gtggggggcg ggcagggcgc ggccatggtc
      241 ctggaacgca cggcctgagc ggccggagga acgaatgccg aagatcgaga agctcttcct
      301 gaagttgcca ccccgctgtc gctcgaagga agtggcgttg caactcgggg cgatgaacga
      361 catgacccgc cgtctcttcg aggaagacct ggaaaaggtg acgaaggacg agctgtgctg
      421 gcagccggcg cccggccgca acaccatcgg catgctgctg gcccacctcg ccatcgtgga
      481 aaccttctgg acgcaggtcg gcgtggaacg gctcgacacg tacgacatca agcacatcct
      541 cggcatcggg atggacgacg acggcattcc ggtgccaccg ggcggtggag cgtatcccga
      601 cctcaaggga aaatccctct cccacttcga gaagctgctc gagaagtcgc ggaagtatgt
      661 cacccgcaag tggtcgccgc tgtcggaccg cgacatgaag aagccggtga atcgcacgcg
      721 ccgggacggc agccatgccg tcaccgatcc gcgctggatc ctctatcaca tggtggagca
      781 ctttgccggc caccaggggc agatccttct tatccgccac gagtaccggg acttccgcgc
      841 cggccgctga tggaaacctt ccggatcgag tccgcccccg acggcatcgc ctggctcgtc
      901 gtcgatgcgc cgggagagaa ggtcaacgtc ctgtcgtcgg cggtgctcgg tgaactgcag
      961 tcccggctcg agacgctggc ggcaagcagc gacgtccgcg ccatcggcgt catcagcggc
     1021 aagccgggca ccttcatcgc cggcgccgac gtggacgaga tcggcgccat cacgagcgcg
     1081 gcggccgggg tggaggcctc acgccgcggc caggccatcc tggggatgct ggccatgccc
     1141 gggaagccga cgctggccgc catcgacggc gcctgtctcg gcggtgggct ggagctcgca
     1201 ctcgcctgtc acttccggat cgccggaaat tcgcctgcca cgcgcctcgg ccttcccgaa
     1261 gtgaaactgg ggatcattcc gggcttcggc ggcacgcaac ggttgccgcg cgtcatcgga
     1321 ttcacgccgg cgctcgatct cgccatgacc ggccgcacga tcgacgcctc ccgggccgag
     1381 cgccttggcc tcgtggatga agccgtgccg cccgccatcc tgcgtgctcg cggcgaggca
     1441 tggttgcgca gggcgctgac cggcgagcgt cacggcgggc gctgggcgcg cctcaagccg
     1501 cgtcgcaagc cgaagctcgg cgtgcgcgtg acggggtgga tgccggtgcg cccgttcatc
     1561 gtcctggcca ccgactggaa actgaagcgc acgctgcatg gcgattaccc cgcgccgcac
     1621 gaagccatgg agtccgtcga ggagtcgttt catacgaagc tcgacaaggg cctggcgcac
     1681 gaggccggcc tcgtcgggcc gctcctcgtg actcccacgt gcaagaatct gacatggctt
     1741 ttccgcgcca acaacgaggc ccgccgtccc ggggccatcc ggaatcccga aaccggcaat
     1801 gccgtccccg cgcgcgaagt ccggaacgcg gcggtcatcg gtgcgggcgt catggggggc
     1861 ggcatcgcgc atctcctcgc cgaacagggg atcccggtcc gactgaagga tctcgacgag
     1921 gcggcgctcg tgaagggaat gtcggccgcg gccggcctcg tggccgccca gcggaagaag
     1981 ggccggatcg atcgccgcgc cgcccgcgac cggatgtctc gcatcgcccc ggctctcacg
     2041 tgggacggct tcggctcgat tgactgcgtg atcgaggccg tcgtggaaga cctcggcatc
     2101 aagcagcgcg tggtggggga gatcgagtcg gtcgtgccgg agcgctgcgt catcgccacc
     2161 aacacgtcct cgctgccgat cggcggcatc gcggcgggtg cacgtcaccc ggatcgaatc
     2221 gtcggcctcc atttcttcaa cccggtccac cggatgccgc tcgtcgaagt catcgccgga
     2281 cgggcgtcgt ccgccgaagc ggtggccacg gcgcatggcc tggctcgccg gctcggcaag
     2341 acaccgatcg tcgtcaccga cacgccgggc ttcctcgtga accgcgtcct caccgcctac
     2401 ctggccgagg cgctccggat gctcgtggaa ggaaccgacc cgcgcgccat cgacaaggcc
     2461 atggtccact tcggcatgcc gatgggaccc tttgcgctcc ttgatcagat cggtttcgac
     2521 accgccagca aggccagcaa ggcgatcgag acgttgaacg aacgctacct cccacgcgcc
     2581 agcgtgatgt ccaggctcat cgaggccggc cgcctcggcg tgaaaaacgg gaaggggttc
     2641 taccgttacc ggaagggaaa gaacatcggc ttcgacaagc acgtcctcgg cctcgtcgag
     2701 catggggaca aatccgaaca cggcgtggaa gacatccagg cccgcctcta tctcccgatg
     2761 gtcaacgagg cggtgcgatg cctggcggct ggcgtggccc gttccccggc cgacgtggat
     2821 ctcgggatgg tcctcggcac cggattcccg ccgttccgcg gcgggccact gcggaacgcc
     2881 gacatcatgg ggatcccggc ggtggtcgag cgcctcaacg aactggccac ccgggtgggg
     2941 gagcggttgt cgcccgagcc gcggctggtg cagatggggc tgtcgggaga gaggttctat
     3001 ccacctgtgt cgcaatagat tagggaaatt gacataccat ctgacgatcg atacagttgt
     3061 ggtatgacga agcagcgatc gatgaagcgc cgccccgaca gggtcacgat ctcgcccaag
     3121 ttccagatcg tcatccccca gcacatccgg gaggagctga agctcgagcc gggtcgcgag
     3181 ctgtacgcgg tggccatcga cggccagatc gtgttcgttc cattgcgccc gctcgaggag
     3241 tacctgggaa ttcttcaggg tccgaatgac ttcgaacgcg agaagcagga tcggttctga
     3301 atgttcctgc tcgacagttc cgcatggatc gagatattca cgaag
//
LOCUS       DASVOD010000448         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_394378, whole genome shotgun sequence.
ACCESSION   DASVOD010000448 DASVOD010000000
VERSION     DASVOD010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394378"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VF720_10200"
     CDS             <1..1810
                     /locus_tag="VF720_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7879772.1"
                     /translation="PAPMALLSLNDVTLRFAGPPLLDQVSLQIDDGERVGLLGRNGAG
                     KSTLLNLLLGTLTPDSGDVVRRPGLRVAGLQQDVPLDLVGTVGQYLHAACGVTSHDSP
                     WEVESRIDGAARDLKLNLESRIETLSAGSKRRVLLAEALVRNPDILVLDEPTNHLDID
                     VIRDLEESLLRRKGTLVFVTHDRSFLRRLATRILDLDRGNLSSYRVPYDQYLEMRDHA
                     REIELQQDAQFDRKLAQEEAWLRQGIKARRTRNEGRVKSLEALRVERGDRREDPGRVK
                     ASLEAADSSGRIVLRTSDAGFGYDGRPIVSHLTLTIRRGDRIGILGPNGSGKTTLIRL
                     LLGELEPQHGSVTPGARLEVGHFEQLHDVLDDSKTVLENLADGRDFLSIGGSERHVVG
                     YLRDFLFTPEQMQGSILKLSGGERKRLQLAKVLSRPCNLLVLDEPTNDLDMETLELLE
                     EILFEFAGTILVVSHDRAFLDNVVTSTLVWEGHGHWREFVGGYSDWLRQRPAPAPPPV
                     IAKAVKVTTERHATTAPRRATFKEKKELEELPGRIEALEAEKQSIFDAMAAPDYHTRR
                     GDEVTKLSTRLTTVEAELEKAYARWMELETIVAGGG"
     gene            complement(1812..2339)
                     /locus_tag="VF720_10205"
     CDS             complement(1812..2339)
                     /locus_tag="VF720_10205"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879773.1"
                     /translation="MSRNGSSALITTERLVIRRLLPGDVDELFAVYGDPESMRWVGDG
                     QPLDRAACEQWVGVTERNVTTRGYGMSVMVDRASGEVVGFVGLVHPGGQELPELKYAL
                     KRDQWGKGLATEAARAMLAWGADRFGLTTIIATIAPENDASRRILEKLGMTEVEPRLD
                     DDGLPTLVFRWVAPG"
     gene            complement(2379..>2734)
                     /locus_tag="VF720_10210"
     CDS             complement(2379..>2734)
                     /locus_tag="VF720_10210"
                     /inference="COORDINATES: protein motif:HMM:NF033208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor E domain-containing protein"
                     /protein_id="HEX7879774.1"
                     /translation="DLSTLVTVVPTVTTVDTLPVFDNNLDYGGPSGRSYFNLPGAAAD
                     SVCLSGAGDLALFAGAGTISLPGNAVDGSFQTGASSWSIGPRPYATITVTYNYRDCSV
                     PVTPSTWSGIKRLLP"
BASE COUNT          470 a          936 c          906 g          422 t
ORIGIN      
        1 tcccgccccc atggcactcc tgagcctcaa cgacgtcacg ctccggttcg caggcccgcc
       61 cctcctcgac caggtgtccc tccagatcga cgacggggaa cgcgtggggc tgctcggccg
      121 gaacggggcg ggcaagtcga cgttgctcaa cctgctcctc ggcacgctca cccccgattc
      181 gggcgacgtg gtccgtcggc ccggcctccg ggtggccggc ctgcagcagg acgtgccgct
      241 ggacctcgtc ggcaccgtcg gccagtacct gcacgccgcc tgcggagtga cgagccacga
      301 cagcccgtgg gaagtggagt cgcggatcga cggcgccgcg cgcgacctga agctgaacct
      361 cgagtcccgg atcgaaacgc tgtcggccgg ctccaaacgc cgcgtgctcc tcgcggaggc
      421 gctggtccgc aacccggaca tcctggtcct cgacgagccg accaaccacc tcgacatcga
      481 cgtgatccgg gatctcgagg aatcgctgct ccgccggaag gggactctcg tcttcgtcac
      541 tcacgaccgg agctttctca gacggctggc cacccgcatc ctggatctcg accgaggaaa
      601 cctgtccagt taccgcgtgc cgtatgacca gtatctcgag atgcgtgatc acgcacgcga
      661 gatcgaactc cagcaggatg cccagttcga caggaagctg gcccaggaag aagcctggct
      721 ccgccagggg atcaaggcgc gccgaacgcg caacgaggga cgggtgaaga gcctcgaggc
      781 cctgcgcgtc gaacggggcg accgccgcga ggacccgggc cgcgtgaaag ccagcctcga
      841 agcggccgac agctcggggc gcatcgtcct ccggacgtcg gacgccggct tcggctacga
      901 cggccgcccg atcgtgagcc atctcacgct caccatccgc cgcggggatc gcatcggcat
      961 ccttggcccc aacggttcgg gaaagaccac gctcatccgg cttctgctgg gcgaactgga
     1021 gccgcaacac gggagcgtga cgccgggcgc ccggcttgaa gttgggcact tcgagcagct
     1081 ccacgacgtg ctcgacgact cgaagacggt cctcgagaac ctcgccgacg gccgcgactt
     1141 cctgtcgatc ggcggttccg agcgccacgt ggtgggctac ctgcgcgact tcctcttcac
     1201 ccccgagcag atgcagggct ccatcctcaa gctctccggc ggcgagcgca agcgcctgca
     1261 gctggccaag gtattgtcgc ggccgtgcaa cctgctggtc ctcgacgaac ccacgaatga
     1321 tctcgacatg gaaaccctgg agctactcga ggaaatcctc ttcgaatttg ccgggacgat
     1381 cctggtcgtc tcgcacgatc gcgcgtttct ggacaacgtc gtcacgtcca cgctcgtctg
     1441 ggaaggtcac ggccactggc gcgaattcgt ggggggatat tccgactggc ttcgacagcg
     1501 gccggcgccg gcccccccgc ccgtcatcgc gaaggcggtc aaagtaacca cggagcggca
     1561 tgccaccacc gcgccccgtc gcgccacgtt caaggagaag aaagagctcg aggaattgcc
     1621 cggccgcatc gaggcgctcg aggcggaaaa acaatcgatc ttcgacgcca tggccgcgcc
     1681 cgattaccac acccggcggg gcgatgaggt cacgaaactg tccacgcgac tcaccaccgt
     1741 cgaagcggaa ctcgaaaagg cctacgcgcg ctggatggag ctggagacga tcgtggccgg
     1801 cggcgggtag ctcagccggg cgccacccac cggaagacca gcgtcggcag gccgtcgtcg
     1861 tcgaggcgcg gttcgacctc cgtcatgccg agtttctcga ggatgcgtcg cgacgcgtca
     1921 ttctcgggcg cgatcgtggc gatgatcgtg gtgaggccga atcgatcggc accccaggcc
     1981 agcatggccc gggcggcttc ggtggcgagg ccttttcccc actggtcgcg cttcagcgcg
     2041 tacttcagct cgggcagctc ctggccgccc ggatgcacga gtccgacgaa accgacgact
     2101 tcgccgctcg cacgatcgac catcacagac atgccgtatc cgcgcgtcgt gacgttccgt
     2161 tcggtcacgc ccacccactg ctcgcaagcg gcgcggtcca gaggttggcc gtcccccacc
     2221 cagcgcatgg attccggatc gccgtagacg gcgaagagtt catccacgtc gccggggagg
     2281 aggcgtcgga ttacgagtcg ttccgtggtg atcagcgccg atgatccgtt ccggctcatc
     2341 tcgcttgatt ggactcccga aagcgcgcag cggcctcctt acggcagcag ccgcttgatc
     2401 ccgctccacg tactgggcgt taccggcacc gagcagtcac ggtagttgta cgtgacggtg
     2461 atggtggcgt aggggcgcgg gccgatcgac cagctgctgg cgccggtctg gaaggagccg
     2521 tcgacggcgt tgccggggag actgatggtt ccggctcccg cgaagagggc caggtcgccg
     2581 gcgcccgaga ggcagacgct gtcggcggcg gcgccaggga ggttgaagta actccggccg
     2641 gaggggccgc cgtagtcgag gttgttgtcg aagaccggga gggtgtcgac ggtggtcacc
     2701 gtcggcacga ccgtgaccag cgtcgagaga tccg
//
LOCUS       DASVOD010000449         5508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1023747, whole genome shotgun sequence.
ACCESSION   DASVOD010000449 DASVOD010000000
VERSION     DASVOD010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5508
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023747"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF720_10215"
     CDS             complement(<1..515)
                     /locus_tag="VF720_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879775.1"
                     /translation="MPGAGRFFLNPLPDRLAALSAVLLLAVVLPVIGCGDDDDDDPDP
                     VEKTIHVVHTAAELDTALMNVTAGDTIEIDGGFSGTQFLMTKGYDFTSETSPFLLRSA
                     PRTTIRPEIVFPANVNGFTFHDHDGTRLTRLSLRGGLTTIVLDDARVLIDTLEIRDPA
                     RDGVESMGSGST"
     gene            complement(567..1187)
                     /locus_tag="VF720_10220"
     CDS             complement(567..1187)
                     /locus_tag="VF720_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7879776.1"
                     /translation="MQEQHMEDAGFVGYLGQKLRRLRKEQNLTQLDLAVQVGITNGQI
                     STIERGVSSPSIATLHKISQVLGVSMGEFFEEEPEGEVQVIRKGRGRKITGTDDNVKV
                     EVLTQRGRHGGVNTLFVEVGSGELKIAPMPHSSDDYVLYVIKGKCNLRVGDESHVLDA
                     SDAVYVKARREQWIKNVGAGPVQAILVSQGALIGSEIQSLLAGAGV"
     gene            complement(1515..2870)
                     /locus_tag="VF720_10225"
     CDS             complement(1515..2870)
                     /locus_tag="VF720_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7879777.1"
                     /translation="MPERRILLVDDDPAARAAVREYLEIHGFAVTEAATAEGTVEAFR
                     SRPPDVVILDYRLPDGHALEILPKLKGIDAEVPILILTGHGSIDLAVTAIQQGAQQFL
                     TKPVQLPALLVMVRNAIESHRRARREAGNTATATRVAPIDPFLGTSPAIRNLKEQAGR
                     VVGSDSPILIQGETGSGKGVLARWLHEHSPRREEPFVDLNCAGLSREFLESELFGHER
                     GAFTGAVAAKKGLLEVADRGSVFLDEIGDVDKDLQPKLLKVLEEKQFRRLGDVKYRSV
                     DIRLIAATHRDLARAVQEGDFRGDLYYRINAIPLTVPSLRDRPEDIPVLARQFLEGFA
                     RDSGRSEIDLTPGGEEALREYAWPGNIRELRNVLERAVLLAGNGPLDRRDLQFTPVPE
                     PEGAGLPAGLTLQEMERRYVEQVLREENGRVENAARRLGIPRSSLYNKLKRWNLNPSR
                     F"
     gene            complement(2839..4404)
                     /locus_tag="VF720_10230"
     CDS             complement(2839..4404)
                     /locus_tag="VF720_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7879778.1"
                     /translation="MPNRAALPTWYDTPEDPDAPLLPMRRRRALVFLLTVIVGFSWLE
                     LTAEGNIHYLQFLILVPALAALLTTPATTAFLAVTAMIATLAVGVVGGISLTTFYVPA
                     AVSILAAGVVGTFTANAQREILLENHRREMEHRRALRESLSLLQATLESTADGILVVD
                     LQQRVTSFNARFAEIWGLSDELLSRRKDEELIEAVLSQLVDPEAFLMRVRELYDRPSE
                     ESFDTLQFKDGRVMERYSKPQMIDTRIVGRVWSFRDVTRAREAEAERARLRDTLARSE
                     SMSAIGELVGGVAHEVRNPLFTISASVDTFEAKFGHRPEFRPYTSALRGSVERLSRLM
                     GQLLDYGRPQQTAPVPMSLAECVQLAIDLLPLRGGDHGIRIENRVPADLPAITIDRYQ
                     VLQVFQNLIENAVQASSDGGTVVITGFRLELEGHDGCECSVDDSGPGFNPGPIGRIFQ
                     PFISERAGGTGLGLSIVQRIVEQNGGRVVAGNRPEGGASVRVWFPAATPSPIDSHALE
                     KGTHAGTPDPTRR"
     gene            4608..5186
                     /locus_tag="VF720_10235"
                     /pseudo
     CDS             4608..5186
                     /locus_tag="VF720_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
BASE COUNT          948 a         1787 c         1820 g          953 t
ORIGIN      
        1 gtagagccgc ttcccatcga ctccacgccg tcgcgggcgg gatcgcggat ctcgagcgtg
       61 tcgatcagga cccgcgcgtc gtcgagcacg atggtggtca gccccccgcg cagtgagagc
      121 cttgtcaggc gcgtgccgtc atggtcgtgg aacgtgaagc cgttcacatt cgccgggaag
      181 acgatctccg gacggatggt ggtgcgcggc gccgaccgga gcaggaaggg gctcgtttcc
      241 gacgtgaagt cgtacccctt cgtcatgagg aactgggtgc cggagaatcc gccgtcgatc
      301 tcgatcgtgt cgccggccgt cacgttcatg agggccgtgt cgagctccgc cgccgtgtgg
      361 acgacgtgaa tcgtcttctc cacggggtcc gggtcgtcgt cgtcgtcatc gccgcagccg
      421 atgactggaa ggacgacagc cagcaggagg accgcggaca gggcggccag gcggtcaggg
      481 aggggattca aaaaaaatcg gccggcaccg ggcatggatt cctccaaccg ggcgccggcc
      541 gaacgagcgt ggttactggt gtggtgtcag actccggcgc cggccaggag cgactggatc
      601 tccgaaccga tgagcgcgcc ctggctgacg aggatggcct gaacagggcc ggcgcccacg
      661 ttcttgatcc actgctcccg tcgggccttc acgtacacgg catcgctggc gtcgaggacg
      721 tggctctcgt cgccgacccg aagattacac tttcccttga tgacatacag gacgtaatca
      781 tccgaagagt gcggcatcgg ggcgatcttc aattcgccgc tcccgacctc cacgaacagc
      841 gtgttcacgc ccccgtggcg gcctcgctgc gtcaggacct cgacctttac attgtcgtcg
      901 gtgccggtga tcttccgccc gcgaccttta cgaatgacct ggacctctcc ttcgggctcc
      961 tcctcgaaga actcgcccat cgacacgccg agcacctgcg agatcttgtg cagggtggcg
     1021 atggacgggg aggagacgcc tcgttcgatc gtcgaaatct ggccgttcgt gatgccgacc
     1081 tgcaccgcga gatcgagctg ggtaaggttc tgttccttgc gcaagcgccg aagtttctgg
     1141 ccgagataac caacgaaacc ggcgtcttcc atgtgctgct cctgcattct gatcaagctc
     1201 cttccgatta caaacacccg cgatcaccgt ccgggaccgc cagatcctgg tgcgtgatca
     1261 ggtctactac caaggagcgt gccagttctc cgggagaatc ggccggggtc cgggaatatt
     1321 cgtccccccc attgagttgc agcgtgaaaa cgttttcaca agggcccgaa ttcattgaat
     1381 ttagatcgct ggcaaatgga tcgtaaattc attggcgaca ctgcacgaat cgcctgatta
     1441 ttcagatgag tggtcgttca agccccatcg gaattgagat tcctcgtgag gattcttaaa
     1501 tctggacgcc aattctagaa tctggacggg ttcagattcc agcgtttcaa cttattgtag
     1561 agcgagctcc gcggtatccc gaggcgccgg gcggcgttct ccacccgacc gttttcctcc
     1621 cgcaggacct gctcgacata ccgccgctcc atttcctgga gggtgaggcc ggccggaagc
     1681 ccggccccct ccggctccgg caccggggtg aattgaaggt cgcgccggtc caggggcccg
     1741 tttccggcca ggagaaccgc gcgttccagc acgttccgga gttcccggat gttccccggc
     1801 caggcatatt cccgaagcgc ctcctcgcct ccgggtgtga ggtcgatttc gctccggccg
     1861 ctgtcccgcg caaacccctc gaggaactgc cgggccagta ccgggatgtc ctccggccgg
     1921 tcgcgaagcg atgggacggt cagcgggatg gcattgatcc ggtagtagag atcgccgcgg
     1981 aaatcgccct cctgcacggc gcgggccagg tcacgatgcg tggcggcgat gagccggatg
     2041 tcgacggagc gatacttgac gtcgccgaga cgccggaatt gtttttcctc gaggaccttc
     2101 aggagcttgg gttggaggtc cttgtccacg tcgccaatct cgtccaggaa gacgcttccc
     2161 cggtcggcca cctcgagcag ccccttcttg gcggccaccg cgcccgtgaa ggcaccgcgc
     2221 tcgtggccga acagctcgct ctcgaggaac tcgcgcgaga gcccggcgca gttgaggtcg
     2281 acgaacggtt cctcgcggcg cgggctgtgc tcgtggagcc agcgcgccag cacccccttg
     2341 cccgagccgg tttcaccctg gatcaggatg ggactgtcgg agcccacgac acgcccggcc
     2401 tgttccttga ggttccggat ggcgggactc gtcccaagga atggatcgat gggggcgacc
     2461 cgcgtggccg tggcggtgtt ccccgcttct cgtcgggccc ggcgatggct ttcgatggcg
     2521 ttccgcacca tcaccaggag ggccgggagt tgcaccggct tcgtcaggaa ctgctgggcg
     2581 ccctgctgga tggcggtgac cgccaggtcg atcgagccat ggccggtcag gatcaggatc
     2641 ggcacttcgg cgtcgatccc cttcagcttc ggcaggattt ccagcgcgtg gccgtcgggc
     2701 aaccggtagt cgaggatgac gacgtccggc gggcggctgc gaaaggcttc cacggtccct
     2761 tcggcggtgg cggcctccgt gacggcgaag ccatggattt cgaggtattc ccgaacggcc
     2821 gcccgggcgg ccggatcgtc atcgacgagt aggatccggc gttccggcat gtgttccttt
     2881 ctcgagtgcg tgtgagtcaa tcggcgaagg cgtcgcggcg gggaaccaga cccgcaccga
     2941 ggcgcccccc tcgggtcggt tgccggcgac gacacgtccg ccgttctgct cgacgatccg
     3001 ttgcacgatc gacagcccga gccccgtccc gccggcccgt tcgctgatga acggctggaa
     3061 gatacggccg atcggccccg gattgaagcc cggcccggaa tcgtcgacgg agcactcgca
     3121 cccatcgtgc ccctcgagct cgaggcggaa cccggtgatc acgaccgtgc ccccgtccga
     3181 ggaggcctgg accgcattct cgatcaggtt ctggaacacc tggagcacct ggtaacggtc
     3241 gatcgtgatg gccggcaggt ccgccgggac acgattctcg atgcggatgc cgtggtcgcc
     3301 tccgcgcagc gggaggagat cgatggccag ctggacgcat tcggccagcg acatcggcac
     3361 cggtgccgtc tgctggggcc ggccgtagtc gaggagctgc cccatcaacc tggaaaggcg
     3421 ctccaccgaa ccgcggagcg cactggtata cggccggaac tcgggacgat ggccgaactt
     3481 ggcctcgaaa gtatcgaccg aggccgagat cgtgaacagg ggattgcgca cttcgtgcgc
     3541 cacgccgccc acgagttctc cgatggccga catcgactcg ctgcgggcca gggtgtcgcg
     3601 caggcgcgcc cgctccgcct cggcctcgcg cgcgcgggtg acatcgcgaa agctccagac
     3661 ccgcccgacg atccgggtat cgatcatctg gggtttggaa taccgttcca tcacccggcc
     3721 gtccttgaac tggagagtat cgaaactctc ttccgatggc cggtcgtaca gctcgcgaac
     3781 ccgcatgagg aaggcctccg gatcgaccag ctgggagaga acggcttcga tcagttcctc
     3841 gtccttccgg cggctcagca gctcgtccga caatccccag atctccgcga accgcgcgtt
     3901 gaagctcgtg acccgctgct ggaggtcaac caccaggatg ccgtcggccg tcgactcgag
     3961 cgtggcctgc aggagggaga gcgattcgcg gagcgcgcgc cggtgctcca tctcgcggcg
     4021 gtggttttcg agcaggatct cccgctgggc gttggccgtg aaggtgccga cgaccccggc
     4081 cgccaggatg ctgactgcgg ccggcacgta gaaggtggtg agggagatcc cgccgaccac
     4141 cccgaccgcg agcgtggcga tcatggccgt tacggcaagg aaggcggtgg tggcgggcgt
     4201 ggtgagcagg gctgccagcg ccggcacgag gatcaggaac tggaggtagt ggatgttccc
     4261 ctcggccgtg agttcgagcc aggagaaacc gacgatgacg gtgagaagga acaccagtgc
     4321 ccggcgtcgg cgcatgggga ggaggggtgc gtccggatcc tccggggtgt cgtaccaggt
     4381 tggcagggcc gcgcggttcg gcatgaatga ggctccggcc gtccggcaac ggggtggccg
     4441 ggagcgcgac gcgcggcggc caggcaaccc ggaacgtgcc cgaagggagt ctagcaacga
     4501 gggggagggg cgtcgagatt cccctaaacc gcggggctcc cggggtcgat aaccccgatg
     4561 tcgcagcagc ggttgccagg atcactcgga cgggaggtta ccttttcatg gtcgtcgtca
     4621 gctattcggg caaggaaatc aacgccaagc tggtctatta cggaccgggt ctctgtggca
     4681 agacgaccaa cctggagcac gtctacgaca gcatcccgtc cacccaccgc ggcaagatgg
     4741 tgtcgatgaa gacccgcacc gagcgtacgc ttttcttcga cttccttccg gtggacctcg
     4801 gcgaactggg cggcttcaag acccgcttcc tcctctacac cgtgcccggc caggtgtatt
     4861 acaacgcgac acggaaactc gtgctgcggg gtgtggatgc ggtggtcttc gtggccgact
     4921 cggcgcgcgg caagatgaac gaaaacctgg agagcctcga gaacctgaag gaaaaccttc
     4981 gggaatacgg cctcgacctg gctaccatgc cgtgggtcct ccagttcaac aagcgcgacc
     5041 tccccgacac gtatacgatc gaagaaatga acgccgccct gaaccccggc ggcatccacc
     5101 acttcgaggc ggtggccacc aacggcacgg gcgtcttcga gacgctgaag ggcgtctccc
     5161 ggctcctgct cgacaagctt tcgcgggaag tgaagctttc gccgaagatc gccgaccccg
     5221 gcatcgaggc aaagaagccc gtcgcggccg aaccgccgaa gaagccggga atcgcgtttg
     5281 caccgggcat gccgggggcg accgatgctc ccgcggcttc gccgtcgcat ccgaagtcca
     5341 ctccaccttc gatggccacc ccgccgccgc tcgcggtgcc gccgtcggtt tcggggacca
     5401 cgtcacagcc ggccagctcc ccgcagcagg ccagctccgc gcagtcggcc agctccgcta
     5461 cgagcagcgc cacgaagccg gctccggcgc cggtggccgc ggcctctt
//
LOCUS       DASVOD010000450         8658 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_707041, whole genome shotgun sequence.
ACCESSION   DASVOD010000450 DASVOD010000000
VERSION     DASVOD010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8658
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707041"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VF720_10240"
     CDS             <1..1378
                     /locus_tag="VF720_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX7879779.1"
                     /translation="GARIEHVRELGGTRILWSALLRGDIDVYAEYTGTLREEILGGGG
                     GTTIASLAAALDSVGVGMSAPLGFNDTYALGMTRRLADSLGIRTIADLAGHPEIRYGL
                     SHEFLDREDGWPGLARAYALPTRHVTGLDHELAYRALMEGQVAVTDLYSTDAEIRRYD
                     LVVLEDVRRWFPEYQAVWVWRRDLVTRAPSAVAALRSVEGRIDDDAMVAMNAAVRIDR
                     KGESRVAAAFLDSIGVVPGIDSGAAAGSPSPDSRWERVGRRTVEHLAMVFASLLMAVV
                     TGVPLGILAARHPVAGRLLLGVTGMLQTIPSLALLVFMLPWLGIGWRPALAALFLYGL
                     FPIVRGTVTGLAGLPGSLRESAEALGLPDFYRLRRVELPLASRSILSGIKTSAVINVG
                     TATLGALVGAGGYGQPILTGIRLDDMGLILEGAIPAALLALLMQTFFDSLEHLFVSEG
                     LRDGGGHP"
     gene            1431..2789
                     /gene="egtB"
                     /locus_tag="VF720_10245"
     CDS             1431..2789
                     /gene="egtB"
                     /locus_tag="VF720_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010045467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX7879780.1"
                     /translation="MAPRRTHDVTAARERGAAPKSHSDRFRQVRAATDRLAARLGIED
                     TVIQSMPEASPTKWHLAHTTWFFETFVLAKGDPAYQSFHPDFNFLFNSYYNAVGPRHE
                     RPRRGLLSRPTLDEVESYRQTVDRLVLAALADGSIEGAGLLPVLEIGLHHEQQHQELI
                     LTDIKHAYWMNPLRPAYQPLDAHAPRVPPGTRADGPPYGGWVHYDGGLVEVGVEDSGF
                     AYDNEGPRHRRWLEPFALSVNLVTNADWLQFMADGGYERPEFWLSDGWSVAKEQGWQA
                     PFYWEKDDSGWRHFTLSGMSTLDTDEPVCHVSFYEADAFARWAGARLPEEAEWETAAR
                     PVIDSFEGATRERLGHFVERGILHPRPAEDSGEPVAPRQLFGDVWEWTSSPYTAYPGF
                     RPVEGALGEYNAKFMCNQMVLRGGSVATPADHIRATYRNFFPPDARWQFMGVRLARGE
                     GR"
     gene            2786..3790
                     /gene="egtD"
                     /locus_tag="VF720_10250"
     CDS             2786..3790
                     /gene="egtD"
                     /locus_tag="VF720_10250"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX7879781.1"
                     /translation="MTPALVEQANRREAARLKLIDLHPAGDGFLADVLLGLSRRSPEI
                     PCKYFYDAAGSVLFDRICELPEYYPTRTELGIMHRHAAAMATRIGPGAVLIEYGSGSS
                     LKTRTLLAALESPGAYVPVDIAKEHLLDAAARLSVDFPRLDILPIAADFTRPFQLPDD
                     VARRGRRVVYFPGSTIGNFTPDEAVALMAGMARLAGPDGRLLLGVDRVKPRAILEAAY
                     NDAAGVTAAFNLNLLTRMNRELGADFDPTRWSHRAFYNEEAARIEMHLESRVLQAVRV
                     GGATFLFEAGDSIRTEYSHKYDRARIDAMAERAGWEVRRSWSDAQEWFSVLELVARRG
                     "
     gene            complement(3792..5252)
                     /locus_tag="VF720_10255"
     CDS             complement(3792..5252)
                     /locus_tag="VF720_10255"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7879782.1"
                     /translation="MPFRALELRPEICTAIDELGYKEPTPIQIKAVPIVMKGRDVIGI
                     AQTGTGKTAAFVWPMLERMMATRPDPAPAIPQPRGLVIVPTRELAVQVYDNLVAYAKY
                     LPIRAMAIYGGVSEFTQLQGLKKGADVIVATPGRLLDFLSTMSINLKNVDFMVLDEAD
                     RMLDMGFIPDVRRILGYLPPKRQTLFFSATFPPVVEKLAKEFLTNPQVVEIGKRATPT
                     ETVAQYVFEVPRNHKAPLLAHILRQYDLKCVLVFVRTKIMADRLSRDLASHGVMVAAL
                     HSNRTQGQRMAALKGFKHGLYPVLVATDIAARGIDVDGISHVVNYDFPNHPEDYVHRI
                     GRTGRAQAIGDALSFVSPEEMPALRTLERFIGRGIPRRVVEGFDPKNAPRGERSGRSD
                     GERGSRSGRGGEGRGEREGRGSRGGGGSDRRRGGSGGDARGGSRGESRTPAPLPPMRH
                     FGHEASRDVVPGHSAPLSPGGETDFGRVPKPGRRRR"
     gene            complement(5347..5874)
                     /locus_tag="VF720_10260"
     CDS             complement(5347..5874)
                     /locus_tag="VF720_10260"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX7879783.1"
                     /translation="MARAARLFATLSLVLGTCAPAFLPTAARAETTVFVVRHAEKVDS
                     SEDPDLDATGRERARLLRDMLRDIPIDSIYSSRYQRALQTVLPTAQLKEQMPVIHTTE
                     RIASITAALRSSAADSVDRYVLAAGHSNTVILWLKGLGIDSIAELMDHEWDNLFIVTI
                     GDGGRSRLLRLHYGY"
     gene            complement(5874..8243)
                     /locus_tag="VF720_10265"
     CDS             complement(5874..8243)
                     /locus_tag="VF720_10265"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX7879784.1"
                     /translation="MTGRQFQSFGIVTAAVMALYAMTACRTVGPGDSGELTAVMVSWG
                     VAHPPGYPLLSLLGNLLHHLPFPGEPAFLLNLMSALFGALAAGVLAMAVTRATDNQSA
                     GLLAGLALGVSRVFWEYSLVVEVFSLNALFGALLLWLLTELVRSRDEGTPSLWTIPLM
                     ALVAAQALTHHLTLVLVAVPVGLTTLLLVARPAENGFEGARIGRAFVLAGAAVLLGLL
                     PLLYLPLASARDPVLAWGEAFSPTGFVRQLLRSAYGSGTLMSPWGVAKLVLDMGAQVA
                     PSLGHHFLRFWMEIPRSLGWLLPFLALAGTVALFRESRSPWRTFLVSWLGMLAIFFLR
                     VNSPLIPLHLGVTERFYILHHVVLAFLGGVGLARTLDFLRERWGETARRAFLALAMVA
                     LVVAPVGLYSRDVSMKDNHFSADLGLNFTTGLPDSSFLLSEGDLLHNAFYYQQLALAR
                     RTDVEFVDLQKLTYEWYVRQIRRRGRFRLPEKMTHYSSEPESQSVDWLKLNTGPGGHE
                     VAAVSVQDVSYMNEWNLLPQGMWWVFRRKSDIVDIDSLAIRAEVIVRGWKLDSLDRHY
                     HERSWETAQRETYWRALAYVGALIQFADDIGYEHPAKGPTGYAAELRARAEAIAGKDA
                     SSVRALEAEMYQRLLVSGLKHFGKIQDPAELAVKMVTLAEEAVAMGPTDPIALGRLAD
                     LLQADPARWSPVRHLELRRRLVDAKPGDEASLGNYVQIVIDGFKNGQVDSATELPGVI
                     ERQKRYIALVLKATELSDEPTLIARLAKWKSYLTDTEKLQARLAAERGK"
     gene            complement(8240..8440)
                     /locus_tag="VF720_10270"
     CDS             complement(8240..8440)
                     /locus_tag="VF720_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009306281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879785.1"
                     /translation="MAERKAFPLRLDPVILAALSKWAADDLRSVNGQIEFLLREALRT
                     AGRLREKGGVRKDGGTGRDGGG"
     gene            complement(8450..>8658)
                     /locus_tag="VF720_10275"
     CDS             complement(8450..>8658)
                     /locus_tag="VF720_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX7879786.1"
                     /translation="QASAVVSARTKIVEGAVGMVEMALAELNKKQVVVLDEERKAAMV
                     SNLLVVLCSDRHTQPVVNTGTLHN"
BASE COUNT         1364 a         2852 c         2994 g         1448 t
ORIGIN      
        1 cggcgcgcgt attgaacatg ttcgtgagct tgggggtacg cgcatcctct ggagtgcgct
       61 cctgcgcggc gacatcgacg tttatgcgga gtacacgggc acgttgcgcg aggaaatcct
      121 gggcggtggc ggtggaacga cgatcgccag cctggccgcg gcgctcgaca gtgtcggcgt
      181 cggcatgagc gcgccgctcg gcttcaacga cacgtacgcg ctcggcatga cccgccgcct
      241 ggccgattcg ctcggcattc gcacgattgc cgacctcgcc ggccatcctg aaattcgcta
      301 tggcctgagc cacgaattcc tcgaccgcga ggatggctgg ccgggccttg cgcgcgccta
      361 cgcgctgccg acgcgacatg tcaccggcct tgatcatgaa ctggcgtatc gggcgctgat
      421 ggaagggcag gtggcggtga ccgacctcta ctccaccgat gccgaaatcc gacgttacga
      481 cctggtcgtc ctggaggacg tgcgccgctg gtttcccgag taccaggcgg tgtgggtgtg
      541 gcgtcgcgac ctggtgacac gggcgccgtc ggcggtggcg gcgctccgct cggtcgaggg
      601 gaggatcgac gacgacgcca tggtggccat gaacgcggcc gtacggatcg atcgaaaggg
      661 tgagagccgc gtggccgcgg ctttcctcga ttccatcggc gtcgtcccgg ggatcgacag
      721 tggtgcggca gccgggtccc catcgcccga ttcccgctgg gagcgcgtcg gtcgccggac
      781 tgtcgagcat ctcgccatgg tcttcgcctc gctcctcatg gcggtcgtca cgggtgtccc
      841 gctcggcatc ctggctgccc gccacccggt ggccggacgc ctgctgctcg gggttaccgg
      901 catgctccag accatcccgt cgctggcact cctcgtcttc atgctcccct ggctggggat
      961 tggctggcgt ccggcgctgg cggccctctt cttatatggt ctcttcccga tcgtccgcgg
     1021 gacggtgacc ggcctggccg gcctcccggg atcgcttcgg gagtcggccg aggcgctcgg
     1081 gctgcccgat ttttaccggc tgcggcgtgt cgaattgccc ctggcgtccc gatcgatcct
     1141 ttccggtatc aagacatcgg ctgtcatcaa cgtggggaca gccacgctgg gagccctggt
     1201 gggcgccgga gggtacggac agccgatcct gaccggtatc cgattggatg acatggggtt
     1261 gatcctggag ggagccatcc cggccgccct gttggcactc ctcatgcaga cattcttcga
     1321 ttcgctcgag caccttttcg tatccgaagg actccgggac gggggaggtc atccctgaca
     1381 cccctccgtc cgacccgata agcgcccgat tgtccggcag ggggtttccg atggcaccgc
     1441 gtcgtaccca cgacgtcacc gccgcgcgcg agcgcggcgc cgctccgaaa tcgcattccg
     1501 atcgcttccg ccaggtccgg gccgccaccg accggctggc cgcccgcctc ggcatcgagg
     1561 acacggtcat ccagtcgatg ccggaggcca gccccaccaa gtggcacctg gcccatacca
     1621 cctggttctt cgagacgttc gtccttgcaa agggagaccc ggcgtaccag tccttccacc
     1681 cggacttcaa cttcctcttc aattcgtatt acaacgcggt cgggccgcgt catgaacggc
     1741 cgcgccgcgg cctcctctcg cgtcccacgc tcgacgaggt ggagtcgtac cggcagacgg
     1801 tcgaccgcct ggtcctggcc gctcttgccg acggttcgat cgagggggcc ggcctcctcc
     1861 cggtgctcga gatcggcctt catcacgagc agcagcacca ggagctgatc ctcaccgaca
     1921 tcaagcatgc ctattggatg aacccgctcc gtccggctta ccagccgctc gacgcgcacg
     1981 ctccccgggt gccgccgggg acaagggcgg acggcccacc gtacggaggt tgggtgcact
     2041 acgacggtgg tctcgtcgaa gtgggcgtcg aggacagtgg ctttgcctac gacaacgagg
     2101 ggccgcggca tcgtcgctgg ctcgagccgt tcgcgctctc ggtgaacctc gtcaccaatg
     2161 ccgactggct tcagttcatg gccgacggag gctacgagcg ccccgagttc tggctgtcgg
     2221 acggctggag cgtggccaag gagcagggtt ggcaggctcc gttctactgg gagaaggacg
     2281 atagcggttg gcgacacttc accctgtcgg ggatgtcgac actcgacacc gacgaaccag
     2341 tctgccatgt gagcttctac gaggccgatg cctttgcccg ctgggccggc gcccgcctgc
     2401 ccgaggaagc cgagtgggaa acggccgccc ggccggtgat cgacagcttc gaaggcgcca
     2461 cccgggaacg gctcggccac tttgtcgagc ggggcatctt acatccgcgc ccggccgagg
     2521 attctggtga accggtcgcg ccacgccaac tcttcgggga cgtgtgggag tggacctcga
     2581 gcccgtacac cgcctatccc ggattccggc cggtggaggg ggcgctgggc gaatacaacg
     2641 ccaagttcat gtgcaaccag atggtgcttc gcggcggttc ggtggccacc ccggccgatc
     2701 atatccgcgc cacctatcgg aatttcttcc cgcccgacgc gcgttggcag ttcatgggag
     2761 tgcgcctggc ccggggagag ggtcgatgac gccggcactg gtcgaacagg ccaatcgccg
     2821 ggaggcggcc cgcctcaagc tcatcgacct ccacccggcc ggcgacggct tcctggccga
     2881 cgtgctcctc ggtctctccc ggcgctcgcc ggagattccc tgcaagtatt tctacgacgc
     2941 ggcggggtcg gtcctcttcg accgcatctg cgaactccct gagtactacc cgacacgcac
     3001 cgagctcggc atcatgcacc ggcatgcggc cgccatggcc acgcggatcg gtccgggcgc
     3061 cgtcctcatc gagtatggct cgggctcgag cctcaagacg aggaccctgc tggccgctct
     3121 cgagtcgccg ggggcgtatg taccggtcga cattgccaaa gaacacctgc tggatgccgc
     3181 ggcccggttg tcggtggatt tcccgcggct cgacatcctc ccgattgccg ccgatttcac
     3241 gcggccgttc caattgccgg acgacgtggc ccgccgcggc cggcgcgtgg tctatttccc
     3301 gggatcgacg atcggaaact tcacgcccga cgaggcggtg gcgctcatgg ccgggatggc
     3361 ccggctggcc ggtccggacg gccgactgct gctgggtgtg gaccgcgtga agccccgcgc
     3421 gattctcgaa gccgcctaca acgacgcggc cggcgtgacc gccgccttca acctgaatct
     3481 tctcacccgg atgaaccgcg agctcggcgc cgacttcgac ccgacgcgct ggagccatcg
     3541 cgccttctac aacgaagagg cggcgcggat cgagatgcac ctcgaaagcc gggtgctgca
     3601 ggcggtgcgg gtgggagggg ccactttcct gttcgaggcc ggcgactcga tccgcaccga
     3661 gtactcccac aagtacgatc gggcgcggat cgatgccatg gccgaacggg ccggatggga
     3721 agtgcgtcgc tcctggtccg acgctcagga gtggttcagc gtgctcgagc tcgtggctcg
     3781 acgcggctga ctcagcgccg gcgacgcccc ggcttcggca cgcgaccgaa atcggtctcg
     3841 ccgcccggcg aaaggggggc cgagtggccc ggcaccacgt cccggctggc ttcgtggccg
     3901 aagtggcgca tcggggggag cggggcgggg gtcctggatt cgccacgaga cccgccacgg
     3961 gcatcgccgc cggagccgcc ccgacgccga tccgatccgc cgcctccacg gcttccacgt
     4021 ccttcccgtt cgccgcgtcc ttcgcctccc cgcccggagc gggatccgcg ttcgccatcg
     4081 gatcggccgg agcgctcgcc gcgcggcgca ttctttggat cgaacccttc caccacccga
     4141 cgcgggatgc cacggccgat gaagcgctcg agcgtgcgca gcgccggcat ttcctccggc
     4201 gagacgaacg agagggcgtc gccgatggcc tgggcgcggc ccgtccggcc gatccggtgg
     4261 acgtaatcct ccggatggtt cgggaagtcg taattcacca cgtgcgagat gccgtccacg
     4321 tcgatgccgc gggccgcgat gtcggtggcc accagcaccg ggtagagccc gtgcttgaat
     4381 cccttgaggg ccgccatgcg ctgcccctgc gtgcggttcg agtgcagggc cgccaccatg
     4441 actccgtggg aggccaggtc gcgcgagagg cggtcggcca tgatcttggt ccgcacgaaa
     4501 acgagcacgc acttgaggtc gtactgccgg aggatgtggg cgaggagggg cgccttgtga
     4561 ttgcgcggca cctcgaagac gtactgcgcc acggtttcgg tgggggtggc acgcttgccg
     4621 atctccacca cctgcggatt ggtcaggaat tccttggcga gcttttccac gaccggcggg
     4681 aaggtggccg agaagaagag cgtctgccgc ttgggcggca ggtagccgag gatgcggcgg
     4741 acatcgggga tgaaccccat gtcgagcatc cggtcggcct cgtcgagcac catgaagtcg
     4801 acgttcttca agttgatcga catcgtggag aggaagtcga ggaggcggcc cggggtggcc
     4861 acgatgacgt cggcgccctt cttgagcccc tggagctggg tgaactcgct gacgccgccg
     4921 taaatggcca tcgcccggat cggcaggtac ttcgcgtagg ccaccaggtt gtcgtacacc
     4981 tgcacggcca gctcgcgggt gggaacgatg acgaggccgc ggggctgcgg gatggccggc
     5041 gcgggatcgg gccgggtggc catcatccgt tccagcatcg gccagacgaa ggcggcggtc
     5101 ttgccggtgc cggtctgggc gattccgatg acgtcgcgtc ccttcatgac gatcgggacg
     5161 gccttgatct ggatcggcgt cggctccttg tagccgagtt cgtcgatggc ggtgcagatt
     5221 tcgggtctga gctccagggc tctaaagggc attggtcctc gcgtgggccg gtcgttcgca
     5281 cgtccgggtt gtagtggcaa acgattgacc atagcaggtt tggacccatg ccgcgaggcc
     5341 ccggggtcag tagccgtagt gcagccgcag gagccgcgaa cgtcccccgt ccccgatcgt
     5401 gacgatgaac aggttgtccc actcatggtc catcagctcg gcgatcgaat cgatgccgag
     5461 ccccttcagc cagaggatga ccgtattgga gtggccggcc gccaggacgt accggtcgac
     5521 cgagtcggcg gccgaactcc ggagggccgc cgtaatggag gcgatccttt ccgtggtgtg
     5581 gatgaccggc atttgttcct tgagctgggc ggtagggagc accgtctgga gggctcgctg
     5641 gtatctggac gagtagatcg agtcgatcgg gatgtcgcgc agcatgtccc ggagtagccg
     5701 ggcgcgctcc cggcccgtgg cgtccaggtc ggggtcctcg gatgagtcga ccttttcggc
     5761 gtgccggacg acgaagaccg ttgtctcggc gcgagccgcc gtcgggagga aggccggcgc
     5821 gcacgtgccg agcaccaggg aaagcgtcgc gaagaggcgc gccgcgcggg ccatcacttg
     5881 cccctctccg ccgccaggcg tgcctgcagc ttttccgtat cggtgaggta actcttccac
     5941 ttggccagcc tcgcgatcag ggtcggttcg tcggacagtt cggtggcctt gagcacgagg
     6001 gcgatatacc gcttctgccg ttcgatcacg cccggcaatt cggtggccga atcgacctgc
     6061 ccgttcttga acccgtcgat gacgatctgg acgtagttcc cgagcgaggc ctcgtccccc
     6121 ggcttggcat cgacgaggcg gcgccggagt tcgaggtgcc ggacgggtga ccagcgggcc
     6181 gggtcggcct ggaggaggtc ggccaggcgt ccgagggcga tcgggtcggt cggtcccatc
     6241 gccaccgctt cctccgccag cgtcaccatc ttgacggcga gctcggccgg atcctggatc
     6301 tttccgaagt gcttgagacc ggaaacgagc aggcgctggt acatctcggc ctcgagtgcc
     6361 cggacggagg aggcgtcctt gccggcaatg gcctcggccc tggcccgcag ctcggcggcg
     6421 tagccggtgg gtccctttgc gggatgctcg tagccgatgt cgtcggcgaa ctggatcagg
     6481 gcgccgacgt aggccagtgc ccgccagtag gtctcccgct gggccgtttc ccatgagcgc
     6541 tcgtggtaat ggcggtcgag cgagtcgagt ttccagccgc ggacgatgac ctcggcgcga
     6601 atggccagcg agtcgatgtc cacgatgtcg gatttccggc ggaagaccca ccacatcccc
     6661 tgcggcagga gattccattc gttcatgtag ctcacatcct gcacgctcac ggccgccacc
     6721 tcgtggccac cgggaccggt gttgagcttg agccagtcga cgctctggct ctcgggttcc
     6781 gagctgtagt gcgtcatctt ttcggggagg cggaagcgac cgcgccgccg gatctgccgg
     6841 acgtaccact cgtacgtcaa cttctggagg tcgacgaatt cgacatccgt ccgccgcgcc
     6901 agcgcgagct gctggtagta gaacgcgttg tggagcaggt ctccctcgga gaggaggaac
     6961 gagctgtcgg ggaggccggt cgtgaagttg aggccgagat ccgccgagaa gtggttgtcc
     7021 ttcatcgaga catcgcggga atagagccca accggcgcca cgaccagggc caccatggcg
     7081 agcgccagga aggcgcgccg ggcggtctcg ccccatcgct cgcgcagaaa gtccagcgtc
     7141 cgcgccagcc cgacgccgcc gaggaacgcc aggaccacgt gatgaaggat gtagaagcgc
     7201 tcggtgacgc cgaggtggag cgggatgagt ggcgagttca cgcgcaggaa gaagatggcc
     7261 agcattccca gccagctcac caggaaggtg cgccacggcg atcgggactc gcggaagagg
     7321 gccaccgtac cggcaagcgc aaggaagggg aggagccagc cgagactgcg cgggatctcc
     7381 atccagaatc ggaggaagtg gtgcccgagc gacggcgcca cctgcgcccc catgtcgagc
     7441 accagcttgg ccacgcccca cgggctcatg agcgtgcccg aaccgtaggc cgagcggagg
     7501 agctgtcgga cgaatccggt gggcgagaat gcttcgcccc aggcgagaac cggatcgcgg
     7561 gccgacgcga gcggcaggta gaggagaggg aggagtccga gaaggaccgc cgccccggcc
     7621 aggacgaagg cgcgcccgat ccttgctccc tcgaatccgt tctcggccgg ccgcgccacc
     7681 aggaggagcg tcgtgagccc gaccggcacg gccaccagca cgagcgtgag gtggtgggtc
     7741 aacgcctggg ccgccaccag cgccatcagc gggatcgtcc agagggaggg cgttccttca
     7801 tcgcgcgagc ggacgagttc cgtgaggagc catagaagga gcgcaccgaa gagcgcgttg
     7861 agcgaaaaga cctcgacgac cagggagtat tcccagaaga cccgggagac gccgagcgcg
     7921 agacccgcaa ggaggccggc cgactggttg tcagtggcgc gcgtcaccgc catggcgagg
     7981 acaccggctg ccagtgcgcc gaagagcgcc gacatgaggt tcagcaggaa ggcgggttcg
     8041 cccgggaagg ggaggtggtg gaggaggttg ccgaggagag aaagaagggg gtagccgggg
     8101 ggatgggcca cgccccacga caccatgacc gccgtcagct cccccgagtc gcccgggccg
     8161 acggtgcggc aggcggtcat ggcgtagagg gccatgaccg ccgccgtcac gatgccgaag
     8221 ctttgaaact gtctcccggt catccgcctc cgtcccggcc ggttccgccg tccttgcgca
     8281 ctccgccttt ttcccgtaac cggccggccg tccgcagcgc ttcgcggagc aggaattcga
     8341 tctgcccgtt gacgctgcgg agatcgtcgg ccgcccattt cgaaagcgcg gcaaggatga
     8401 cgggatcgag ccgaagaggg aaggcctttc gttccgccat gtccgtaacc tagttgtgga
     8461 gcgtgcccgt gttcaccacc ggctgcgtat ggcggtcgct gcagaggacg accaggaggt
     8521 tgctcaccat ggccgccttc cgctcctcgt cgagcacgac cacctgcttc ttgttcagtt
     8581 cggccagggc catctccacc atgccgacgg cgccctccac gatcttggtg cgggccgaca
     8641 ccaccgcgct ggcctgct
//
LOCUS       DASVOD010000451         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_238780, whole genome shotgun sequence.
ACCESSION   DASVOD010000451 DASVOD010000000
VERSION     DASVOD010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238780"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF720_10280"
     CDS             <1..413
                     /locus_tag="VF720_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879787.1"
                     /translation="ELDQSLLELKRIVKPGGELLISLDNRQNPVVALRNAIPFHWLRR
                     LRIMPYYVGFTCGPHGLRRRLEEGGWEIREMGAILHCPRFLSIHATLLLEKWASPALQ
                     QKFIGWLNGFERLASWPTRYFTGSFVTARAVRRA"
     gene            425..1291
                     /locus_tag="VF720_10285"
     CDS             425..1291
                     /locus_tag="VF720_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7879788.1"
                     /translation="MSDRIRVSLLRGVCQIPAYVAFEKNFLGDEGLDATLDVAATAWL
                     VPQKLMAGESQFAVIPWTRVAAAEANDLPLCLLAGSGHEEVAIVLRNGIEPKDVKRVA
                     VPVRGGMKDLTAMGLLESLGWQDAQLVRLPSGDGAILAFVGQGVDAASMVEPYATMLE
                     EMGMGRVIRRTGDVWKGAPGCSLTTTVPFKEAEPETVQRFVNAFARGVQFVSESPEES
                     ATIAADYIGVHPKFILAALRKNRPDIDAIRNHEAMLGVLALMQKLKYVDRRPEHFSDL
                     SFLDRVAAAANR"
     gene            1364..>1683
                     /locus_tag="VF720_10290"
     CDS             1364..>1683
                     /locus_tag="VF720_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879789.1"
                     /translation="MRAPITREAAHERLARQMAGRGAMFLESTRRQIYANPRSPYRAL
                     LLAVGCDHAELARRVTTLGLEATLAGLADAGVRVSLEEFKRQRPIERESLRLTPDESD
                     FDNP"
BASE COUNT          269 a          577 c          565 g          272 t
ORIGIN      
        1 ccgaactcga ccagagcctc ctggagctga agcgcatcgt gaaaccggga ggcgagcttc
       61 tcatttcgct cgacaaccgg cagaacccgg tggtggccct gcgcaatgcc atcccgtttc
      121 actggctgcg ccggctgcgc atcatgccgt attatgtcgg cttcacctgc gggcctcacg
      181 gcctccgccg tcggctggaa gagggcggct gggaaatccg cgagatgggg gccatcctcc
      241 attgcccgcg cttcctgtcg atccacgcca cgctgctgct cgagaagtgg gcgtcccccg
      301 cgctccagca gaagttcatc gggtggctga acggtttcga acgactggcc tcgtggccga
      361 cgcgctattt caccggcagt ttcgtgaccg cccgcgccgt gcgccgggcc tgaaaggacc
      421 ctccatgtcg gaccggatcc gcgtttccct ccttcgcggc gtttgccaga tcccagccta
      481 cgtggccttc gagaagaatt tcctgggcga tgaagggctc gacgccacgc tcgacgtggc
      541 cgccaccgcc tggctcgtgc cgcagaagct gatggcgggc gagagccagt tcgccgtcat
      601 cccctggacg cgcgtggccg ccgccgaggc caacgatctg ccgctttgtc tgctggccgg
      661 atcgggccat gaagaagtgg cgatcgtgct ccgcaatgga atcgagccga aggacgtgaa
      721 gcgggtggcc gtgccggtgc gtggcggcat gaaggacctc acggccatgg gcctcctcga
      781 atcgctcggc tggcaggacg cgcagctcgt gcgccttccg tcgggggacg gagccatcct
      841 ggccttcgtg gggcagggtg tcgacgccgc gtcgatggtg gagccgtacg ccacgatgct
      901 agaggaaatg gggatggggc gcgtcatccg ccgcacgggc gacgtgtgga aaggcgcacc
      961 cggttgttcg ctcaccacca ccgtgccgtt caaggaggca gagccggaaa cggtgcagcg
     1021 cttcgtcaac gccttcgcgc ggggcgtgca gttcgtgagc gaatcgcccg aggaatcggc
     1081 cacgatcgcg gccgactaca tcggcgttca cccgaagttc atcctcgcgg ccctcaggaa
     1141 gaaccgcccg gacatcgacg ccatccgcaa tcacgaagcg atgctgggcg tgctggcgct
     1201 gatgcagaag ctgaagtacg tcgatcgtcg tcccgagcac ttcagcgacc tgtcgttcct
     1261 cgaccgggtg gcggccgccg ccaaccgctg accggacggc gccgcactga acaggggaca
     1321 gatcggccag ctccttcggc tcggcctggc catgcccggc cgggtgaggg ccccgatcac
     1381 gcgcgaggcc gcccacgagc gcctggcgcg ccagatggcc gggcgtggcg cgatgttcct
     1441 cgagtcgacg cgccggcaga tctacgccaa tccccggagc ccataccggg cgctgctcct
     1501 cgcggtcggc tgtgatcacg ccgagctggc gcgacgtgtc acgacgctgg gcctcgaggc
     1561 aacgctggcc ggcctggccg acgcgggcgt gcgcgtctcg ctcgaggaat tcaagcgcca
     1621 gcgccccatc gagcgtgaat cactccgcct cacacccgac gagtccgatt tcgacaaccc
     1681 gag
//
LOCUS       DASVOD010000452         4360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_238790, whole genome shotgun sequence.
ACCESSION   DASVOD010000452 DASVOD010000000
VERSION     DASVOD010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238790"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            274..1425
                     /locus_tag="VF720_10295"
     CDS             274..1425
                     /locus_tag="VF720_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879790.1"
                     /translation="MSTRAGSRRSLASRLADAIVLLFGVASVLTVAEVAVRPFYPAAP
                     DVLHRQVTHRASQRFGWELVPDPGSVSFTAPAPIDVGGHRDQTIQHSMAMEFNPPPGA
                     AARWLVAGGGNSFGVAVEAGDAFPYLAAGGGTEPPAALVNTGVEGYDLGQKTRRIEAE
                     APAVLPTVILLEIDAADLPAGGTRDSTDLASRFAVLEHRINRAPPPDRDWYGRLLGRS
                     RLASLLDGRARAFLQLGRRLPAAAPSPGRREVRAIDLLLGRETPVIDTAWQTLGAELD
                     RIARTADRVRARVCVVAMPMPAQLRRPYPRATFQSRLERLCRERGFVFVDPLPVLREK
                     RRTGTRTYLPRLPYLNEVGHGAVAEAIRRELETTLAREDHWRLPSRLEE"
     gene            complement(1428..2336)
                     /locus_tag="VF720_10300"
     CDS             complement(1428..2336)
                     /locus_tag="VF720_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX7879791.1"
                     /translation="MDERRPVWDEIGANRFKSFLLMVGFGVLVGLAGWTFGELTGAGP
                     AGSLVSLAFAGVAAFSTYWYCDRIVLAASGAKPVDPLKHRALVNLVEGLAIAAGLPVP
                     RIFTIDDTAPNAFATGRDPKHAAIAVTTGLLDKLDRLELEGVLAHEMSHIANFDIRMT
                     TLAGVMVGSVALLSDWMMRSLRWGMWSGGGRSRRSDGALPIALVAIVFAILAPIAAQL
                     LRMAVSRQREYLADANGALLTRFPEGLARALEKLAADREPLEVANRATSPLFIVNPLH
                     DHKPGRAGLFDTHPPIEERIRRLRAM"
     gene            complement(2339..2905)
                     /locus_tag="VF720_10305"
     CDS             complement(2339..2905)
                     /locus_tag="VF720_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019266545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX7879792.1"
                     /translation="MGGLMIFVLIFLGLIAAIAFWFIGMYNRLVSLRNRIENAWSQID
                     VQLKRRVDLIPNLVETVKGYASHEKEAFERVIQARSALMNAGSNVTAQAEANNMLTGA
                     LKSLFALAEAYPELKANQNFLMLQEELSGTEGKIAYSRQFYNDTVLQYDNAIEMFPTN
                     ILAGMFGFQPKPYFETPAGEREPVKVQF"
     gene            3042..>4360
                     /locus_tag="VF720_10310"
     CDS             3042..>4360
                     /locus_tag="VF720_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX7879793.1"
                     /translation="MPPTTNALSRRGLLADLKPMLALAGPVVAAELGWMFMGLVDLWF
                     VGKLGPLALGGVSIGNTVFIAVTIFGIGMLLGLDYLVAHAHGGGKADEANRSLLTGVY
                     MALILSGVLMTLLILGLPHLDRFGIAPDVVAEARPYLLTITLGMPPLLLFVALRRYLQ
                     AVGKVTAIMFALLSANILNALGGYALIFGNWGFPVLGVAGAGWATCIARFWMLGVVVV
                     AILMKEKKHSTGLWSTPPALETARLSRLTQLGLPAAVQVTLEVGVFAAATILAGRLGT
                     IPLAAHQVALSIASLSFMVPLGISSAGAVRVGQAVGAGDKRQAARAGWTALFTSAAFM
                     TCSGLVFAVFPKPFVDAFTDDPEVLRTGTSLLRIAALFQLFDGLQVTASGVLRGTGET
                     RLPMLVNLIGHWAIGLPIGVTLAFVMGKGILGLWGGLSAGLIVVSILL"
BASE COUNT          639 a         1465 c         1510 g          746 t
ORIGIN      
        1 ccaggaactt gtcgacgaat ccatcgaact cggcccgggg ggtggcgaag gccgacgcgg
       61 tgaccgtgga cgagagcgcc aggaaaaggg cgaggaaagg aagcacgagg gagcggcaac
      121 gggcgagggg cccgatgggc cgacgggaca tcgagacgat ctccttcggg atggatcccg
      181 gatacaggaa tggccggccc gacgactggc ccggcgtcac gagcggggaa tgctatcatg
      241 ccgcccaggt cgtcacaccc cggaggaacc gagatgtcga ctcgcgccgg cagccgccgg
      301 agcctcgcct cgcgacttgc cgacgccatc gtcctgttgt tcggcgtcgc ttcggttctc
      361 accgtggccg aggtggccgt gcgcccgttc tacccggccg ctcccgatgt gctgcaccgc
      421 caggtcaccc atcgcgcttc acagcggttc ggttgggaac tggtgccgga tccgggttcg
      481 gtatccttca ccgctccggc gccgatcgac gtcggtggcc accgcgatca gacgatccag
      541 cattcgatgg ccatggagtt caatccgcca cccggagcag cggctcgctg gttggtggcg
      601 ggaggcggca actcattcgg cgtggccgtc gaggcgggag atgccttccc ctacctggct
      661 gcaggcgggg gaacggagcc gccggccgca ctggtgaaca cgggcgtcga gggatacgac
      721 ctcggccaga agacgcgccg gatcgaggcc gaggcccctg cggtgctgcc caccgtcatc
      781 ctcctcgaaa tcgacgcagc ggacctgccg gctggcggca cccgtgattc caccgatctg
      841 gcaagtcgtt tcgccgtact cgagcaccgg atcaaccggg cgccgccgcc ggatcgcgac
      901 tggtacggcc gactgctcgg caggagtcgc ctggcaagcc ttctggacgg ccgggcgcgg
      961 gcgttcctcc agctgggccg gcggctgccg gcggccgccc cgtcgcccgg ccgtcgggag
     1021 gtccgggcca tcgatctcct gctcgggcgg gagacgccgg tcatcgacac ggcatggcag
     1081 accctgggcg ccgagcttga tcgtatcgcc cgaacggcgg atcgggtgcg cgcgcgcgtc
     1141 tgcgtcgtgg ccatgccgat gccggcccag ctccgccggc cgtatcctcg agccacgttc
     1201 cagtcacggc tcgaacgtct ctgccgggag cgcggttttg ttttcgttga tccgttgccc
     1261 gtgctccggg agaaacggcg gaccggcacg cgcacttacc tgcctcgcct gccctatctc
     1321 aacgaagtcg gccatggtgc cgtggccgag gccatccgga gagaactgga aacgacgctc
     1381 gcccgcgagg accactggcg cctcccgagc cgcctggagg agtagcgcta catcgcccgc
     1441 aggcggcgga tgcgctcctc gatcggcggg tgcgtgtcga acagcccggc gcgccccggt
     1501 ttgtggtcgt gcagtggatt cacgatgaag aggggcgagg tggcccggtt cgccacttcc
     1561 agtggctccc tgtcggcggc cagcttctcg agcgcgcggg ccaggccttc gggaaagcgg
     1621 gtgaggagcg ccccgttggc gtcggccagg tattcgcgct ggcgcgacac ggccatccgg
     1681 aggagctggg ccgcaatcgg ggccaggatg gcgaagacga tggccacgag cgcgatcggg
     1741 agcgccccgt ccgaccgacg cgagcgcccg ccaccgctcc acatccccca gcggaggctc
     1801 cgcatcatcc agtcggagag gagcgccacg ctcccgacca tgacgccggc gagcgtggtc
     1861 atgcggatgt cgaagttggc gatgtgcgac atctcgtgcg ccaggactcc ttcgagctcg
     1921 aggcggtcga gcttgtcgag caggcccgtc gtcaccgcga tggcggcgtg cttcgggtcg
     1981 cgcccggtgg cgaaggcgtt gggcgccgtg tcgtcgatgg tgaagatgcg ggggacgggg
     2041 aggccggccg cgatggccag cccctcgacc aggttgacga gcgcccggtg tttcaacgga
     2101 tcgaccggct tcgcgccgct ggcggcgagc acgatccgat cgcagtacca gtaggtgctg
     2161 aaggccgcca cgccggcgaa cgccagcgac acgaggctcc ctgccggtcc ggcacccgtg
     2221 agttcgccga acgtccaacc cgcgaggccc acgaggacgc cgaacccgac catgagcagg
     2281 aagctcttga agcggttggc cccgatctcg tcccagacag gccggcgttc gtccatccct
     2341 agaactgcac cttcaccggt tcgcgctcgc cggccggcgt ctcgaagtag ggcttcggct
     2401 ggaagccgaa catgccggcc aggatgttgg tcgggaacat ctcgatggcg ttgtcatact
     2461 ggagcacggt gtcgttgtag aactggcgcg agtaggcgat cttcccctcg gtgccggaaa
     2521 gctcctcctg aagcatcagg aagttctggt tggccttgag ctcgggatag gcctccgcca
     2581 gtgcgaagag gctcttgagc gcgccggtca gcatgttgtt ggcctctgcc tgcgcggtga
     2641 cgttcgagcc ggcgttcatc aaggcgctgc gggcctggat gacccgctcg aacgcttcct
     2701 tctcatggga ggcgtaaccc ttgaccgtct cgaccaggtt ggggatcagg tcgacgcggc
     2761 gcttcagctg cacgtcgatc tggctccagg cgttctcgat ccgattgcgg agcgacacga
     2821 gccggttgta catgccgatg aaccagaaag cgatcgccgc gatcaggccg aggaaaatca
     2881 gaacgaagat catgagtcct cccactgagt tcgtccgcga ggagcggtgc ccccgaaccg
     2941 ggtaatccgc cgcgcggtgc ccattacgcc cgtcgccggg agaggttcaa tcgaatgtcg
     3001 gcatgctagc cgccgcggtg tccctcggct agtgtgcccg gatgcccccg accacgaacg
     3061 ccctctcccg acgcggcctc ctggccgacc tgaagccgat gctggccctg gccggccccg
     3121 tcgtggccgc cgagctgggg tggatgttca tggggctggt cgacctctgg ttcgtcggca
     3181 agctcggccc attggccctg gggggggtgt cgatcggcaa caccgtcttc atcgccgtca
     3241 cgatcttcgg aatcgggatg ctgctgggcc tcgattacct ggtggcgcat gcccacggcg
     3301 gcggcaaggc cgatgaggcc aaccgctcgc tcctgaccgg cgtctacatg gcgttgatcc
     3361 tgtcgggcgt gctgatgacg ctgctcatcc tcggcctccc gcacctcgac cggttcggca
     3421 tcgcaccgga cgtggtggca gaggcgcggc cgtatctgct gacgatcacg ctcggaatgc
     3481 cgcctctcct gctcttcgtg gcgcttcgcc gctatctcca ggccgtcggc aaggtgacgg
     3541 cgatcatgtt cgccctgctc agcgccaaca tcctgaatgc cctgggcggc tacgcgctga
     3601 tcttcggcaa ctggggattt cccgtgctcg gcgtggccgg cgccggctgg gccacctgca
     3661 tcgcccgctt ctggatgctc ggcgtggtgg tcgtggccat cctcatgaag gagaagaagc
     3721 actccaccgg cctctggtcg acgccgccgg cgctcgagac ggcgcgtctt tcccggctca
     3781 ctcagctcgg cctgccggcc gccgtccagg tcacgctcga agtcggcgtc ttcgccgccg
     3841 ccaccatcct ggccggccga ctcggcacga tccccctggc cgcccaccag gtggccctgt
     3901 cgatcgccag cctctccttc atggtgccgc tcggaatttc gtcggcgggc gccgtccggg
     3961 tcggtcaggc cgtcggcgcc ggggacaagc gccaggccgc ccgggcgggc tggacggcgc
     4021 tgttcaccag cgccgccttc atgacgtgct cgggacttgt cttcgccgtg ttcccgaagc
     4081 cgttcgttga tgcgttcacc gacgatcccg aggttcttcg aaccgggacg agcctgctcc
     4141 gtatcgcggc gctgtttcaa ctgtttgacg gactgcaagt gaccgcctcc ggcgtcttgc
     4201 gcggcaccgg tgaaacacgc ctgccgatgc tggtgaactt gatcggccac tgggcgatcg
     4261 gattgccgat cggggtcacg ctggccttcg tgatggggaa ggggatcctg gggttgtggg
     4321 gtgggctgtc ggcggggttg atcgtggtgt cgattctgct
//
LOCUS       DASVOD010000453         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_864688, whole genome shotgun sequence.
ACCESSION   DASVOD010000453 DASVOD010000000
VERSION     DASVOD010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864688"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VF720_10315"
     CDS             <1..942
                     /locus_tag="VF720_10315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7879794.1"
                     /translation="PGGSQPAPRYGHGGIYDPIRDEVIFYGGYSSGCQYSVSVWALPM
                     SGPLTWRNPSTTGWGGGVYQVACGYDAPTRRMVAFGGDYHLSCGQPQWNFDASANTMA
                     GFNLDGNTWSPIWPNAAGPIHHESSAESAWDSTRRRMVAIRREAPLFNPPETPDGLVV
                     AWVPTEARWDTLAPAGTGPLLARALLYDPVDDQLLVSSVNPSQGWFLLSFAATSSVPT
                     DNAATRLGLRVHPNPTAGGSTIRFVLPTAGRARISIVDVRGRLIAVLDDREWSAGTHE
                     SRWERLGMDGTPVASGLYFVQVRSAGGTRAARLVVID"
     gene            1224..1850
                     /locus_tag="VF720_10320"
     CDS             1224..1850
                     /locus_tag="VF720_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879795.1"
                     /translation="MRPRSIHRQSSPALVVATIAVTISLAGCGTHDAPVAPKAGFEDA
                     GTTTPLPPAEAIAARRPETIDAIDAEDVSRTQIIRAASGGVITAGRHRLTIPPGALKQ
                     DTSIQLKDVSGSEGYVACEAYPEGLTFEKPVLLETFIGDIKNPFGYTIFWIANPGKSN
                     ENWVDMQATVTPDGQSLEAWLSHFSSYAPGKAGWTPRRGVPGHFRNEG"
BASE COUNT          305 a          647 c          611 g          311 t
ORIGIN      
        1 cccggcggct cccaacccgc gccgcggtat ggccacggcg gaatctacga cccgattcgc
       61 gacgaagtca tcttctacgg cggatactcg agcggttgtc agtactcagt aagcgtatgg
      121 gccttgccga tgtccggtcc gctcacctgg cgcaacccga gcaccacggg ttggggtggc
      181 ggggtgtacc aggtggcgtg cggttacgac gcgccgactc gccggatggt cgccttcggc
      241 ggcgactatc acttgagctg tggccagcca cagtggaact tcgacgcttc ggccaacacg
      301 atggccgggt tcaacctcga cgggaatacg tggtcgccga tctggccgaa cgctgcgggc
      361 ccgatccatc acgagtcgag cgccgagagt gcctgggact ccacgcgtcg gcgaatggtg
      421 gccatacggc gggaggcgcc gctcttcaat cctcccgaga cgcccgacgg cctggtcgtt
      481 gcctgggtgc cgaccgaagc ccgctgggat acgctggctc cggccggaac ggggccgctg
      541 ctggcccggg ccctcctgta cgacccggtc gatgaccaac tgctcgtgag ctccgtgaac
      601 ccttctcaag gttggttcct cctttccttt gccgccacgt cgtccgtacc gacggataat
      661 gccgcgaccc gtctcggcct gcgcgtgcac cccaacccga ccgccggtgg ctcgacgatc
      721 cgcttcgtgc tgccgaccgc aggccgggcc cgaatttcca tcgtggacgt ccgcggtcgg
      781 ttgatcgccg tgctcgacga ccgcgagtgg agtgccggca cgcatgaatc gcggtgggaa
      841 agactcggga tggatggcac acccgtcgcg tccggactct atttcgtgca ggtccgtagt
      901 gccggcggga cgcgcgcagc gcggcttgtc gtcatcgatt agtccggccg ggctggggca
      961 ttgccgccac caagtggctg aacagagccg aatcagcgca attcgttgac caccttgcac
     1021 ctgcccgaag cgtcggaacg acggcggggc tttgcacccg atcgcgtcgc cgattccgga
     1081 cggttacccg acgcctcgaa accagcaact gtcggggacg caactgtttc ggaaatagct
     1141 tgcacgggcg cgcgcgcgct ggcacgtccc ctgcgttact cctggccgca cacgttccgt
     1201 cgttcctttc gcggaggcag ttcatgcggc cacggtcgat ccatcgccag tcgagtcccg
     1261 cgctcgtcgt cgcgacgatt gccgtgacca tctcactcgc cggctgcgga acccatgatg
     1321 cgccggtggc gccgaaggcc gggttcgagg acgccgggac caccacgccg ctaccaccgg
     1381 ccgaggcgat agccgccagg cgccccgaga cgatcgacgc gatcgatgcc gaagacgtct
     1441 cgcgtacgca gatcatccgt gccgccagcg gcggcgtgat caccgccggg cggcatcgcc
     1501 tcacgatccc gccgggcgcc ctgaaacagg acacctcgat ccagctcaag gatgtatccg
     1561 gttcggaggg gtacgtggcg tgcgaggcat atcccgaggg gctcacgttc gagaagccgg
     1621 tgctcctcga gaccttcatc ggcgacatca agaacccgtt cggctacacc atcttctgga
     1681 tcgccaaccc gggcaagagt aacgagaact gggtggacat gcaggccacc gtcactcccg
     1741 acggccagag cctcgaagcc tggctctccc acttctcgag ctatgcgccg ggcaaggccg
     1801 gctggacgcc gcggcgcggc gtaccgggcc acttccgcaa cgaggggtag gggcgcgccg
     1861 ccgggaccca tagt
//
LOCUS       DASVOD010000454         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_238799, whole genome shotgun sequence.
ACCESSION   DASVOD010000454 DASVOD010000000
VERSION     DASVOD010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238799"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1694)
                     /locus_tag="VF720_10325"
     CDS             complement(<1..>1694)
                     /locus_tag="VF720_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7879796.1"
                     /translation="SGVVIAVLDTGVNDQPDQVNPGYPGHESLAGKFLAGGEFWTGQA
                     LLNTALDASTNPQDHGGAASSYHATHVAGSAMGTGGQGGFFRGVAPAARLVDCKVLSD
                     AGASVGGSERGMEWVLHNRNKLWDGLPPGSPWQGIDVVNMSLGSPTSNDDGSSAAAQL
                     VNQLVDAGIVVCIASGNDDRGTGAPGSLTEAGMASPASADKCIAVGASSTAHTLNRLD
                     DRVTDFSNEGPRLSDGDADFSDEMKPNLVAPGAGILSAGGDFTSDGTNYSQLSGTSMA
                     CPHAAGCAALLRQADPALTPLQVRTILQNTAEHKIPSVKGAFRTYAASTDPNYDPGSG
                     WGLIDVYAALKEVQNSTSGVQVVQFRPVAHPEAGRIDVTWVTQREYPFLGFNVYRAPD
                     VAGAPGAFVKINPVLVPPSPNGDPILQNDDNRTPYLYQDTDPALVLGRAYWYRVEWVD
                     LLSGGHFELPAPAVYGFAPRVATVFYRIAHNAPDNDLFVRVGTDAMYSPGSLGTADFE
                     VQGLPEGQQDSAAVVFPTPDNTGTSTLGTIEHYWSIGFSSADGVGPYLPPSIAHPW"
BASE COUNT          241 a          538 c          635 g          280 t
ORIGIN      
        1 aaccaggggt gggcgatgct cggcgggagg tacggtccca caccgtccgc cgagctgaat
       61 ccgattgacc agtagtgctc gatcgtgccg agggtcgagg tgccggtgtt gtccggagtg
      121 gggaagacga cggccgcgct gtcctgctgt ccttcgggaa gaccctgcac ctcgaagtcg
      181 gcggtgccga ggcttccggg cgagtacatg gcatcggtgc ccacgcgcac gaagaggtcg
      241 ttgtcggggg cgttgtgggc gatgcggtag aagacggtgg ccacccgcgg cgcgaagccg
      301 tacacggccg gcgccggtag ctcgaagtgc ccgcccgaca ggaggtcgac ccactcgacg
      361 cgataccagt acgcccggcc aaggacgagc gccgggtcgg tgtcctggta gaggtacggc
      421 gtgcggttgt cgtcgttttg tagaatggga tcgccattcg ggctgggggg cacgaggacg
      481 gggttgatct tcacgaaggc gcccggcgcg cccgccacgt ccggtgcgcg gtacacgttg
      541 aatccgagga acggatactc gcgctgcgtg acccacgtca cgtcgatgcg gccggcctcg
      601 gggtgcgcca ccggccggaa ctgcaccacc tgcacgccac tggtggagtt ctgcacctcc
      661 ttgagtgccg cgtacacgtc gatcagcccc cagccggaac ccggatcgta gttggggtcg
      721 gtgctggcgg cgtaggtgcg gaacgccccc ttcacgctcg gaatcttgtg ttcggcggtg
      781 ttctggagaa tcgtgcggac ctggagcggc gtgagggccg gatcggcctg gcggagcagg
      841 gcggcgcagc cggcggcgtg agggcaggcc atgctcgttc cggagagctg gctgtagttg
      901 gtgccgtcgc tggtgaaatc gccgccggcc gagaggatgc cggcgcccgg ggccaccagg
      961 ttcggcttca tctcgtcgga gaagtcggca tccccgtcgg agaggcgggg cccttcgttc
     1021 gagaaatcgg tgacgcggtc atcaagccgg ttcagcgtgt gggccgtgct gctggcgccg
     1081 accgcgatgc acttgtcggc ggaggccggc gaggccatgc cggcctcggt gaggctcccc
     1141 ggggcgccgg tcccgcggtc gtcgtttcca gacgcgatgc agaccacgat cccggcgtcg
     1201 acgagctgat tcaccagctg ggccgcggcg ctcgagccgt cgtcattcga ggtgggggag
     1261 ccgagcgaca tgttcacgac gtcgattccc tgccatgggc tcccgggggg gaggccgtcc
     1321 cacagcttgt tgcggttgtg gaggacccat tccatgccgc gctcgctgcc gccgaccgaa
     1381 gcgccggcgt ccgaaagcac cttgcagtcg acgagccgtg cggcgggcgc cacgccgcgg
     1441 aaaaatccgc cctggccgcc ggtacccatc gccgatcccg ccacgtgggt ggcgtggtag
     1501 ctcgaggccg cgccaccgtg gtcctgtgga ttggtgctgg cgtcgagggc cgtattgagg
     1561 agcgcctgcc ccgtccagaa ttcgccgcct gccaggaact tgccggccag cgattcgtgg
     1621 cccgggtacc cggggttcac ctggtccggc tggtcgttga cgccggtgtc gagcacggcg
     1681 atcacgacgc cgct
//
LOCUS       DASVOD010000455         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_655420, whole genome shotgun sequence.
ACCESSION   DASVOD010000455 DASVOD010000000
VERSION     DASVOD010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655420"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VF720_10330"
     CDS             complement(<1..298)
                     /locus_tag="VF720_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX7879797.1"
                     /translation="MSGVRVLVGTRKGAFVLTADGKRDRWQVSGPHFPGWEIYHLKGS
                     PADPDRLYASQTSGWFGQVVQRSNDGGNTWEAVGNKFEYDGVAGTHQWYDGTQHP"
     gene            complement(375..890)
                     /locus_tag="VF720_10335"
     CDS             complement(375..890)
                     /locus_tag="VF720_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7879798.1"
                     /translation="MSAMTTERDGAIQAIRYQAQLVHRVVRRNVEGITHEESLLQPEL
                     GGNSLNWIMGHLVWAYAGALPLVRQTPMLDKDHLSQYARGGPPLTDPSRAVDFGQLVA
                     AWDDSSRRMDDGLAGFPVETLAQPAPGSPTNNPDETVGSLLATIMFHQAYHTGQTAVL
                     RRVIGKPGAIK"
     gene            1038..1469
                     /locus_tag="VF720_10340"
     CDS             1038..1469
                     /locus_tag="VF720_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX7879799.1"
                     /translation="MALDFLSPLHKASRQISVYLETRTRELGVSPLEGHVLTYLRSYA
                     PASIRDLVRVFGVKQSTFTSLLDRLERAGYAQRTINPDDRRSFLVHITDKGSELASRI
                     TRLLRTLEDDIRERVEGRDVKGFQAVMQAVEEITHVRLRDR"
     gene            1605..>1881
                     /locus_tag="VF720_10345"
     CDS             1605..>1881
                     /locus_tag="VF720_10345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7879800.1"
                     /translation="MSKRFGGIAAVDQVSFQVDRGQVVGFLGPNGAGKSTTMRMITQF
                     IEPDAGSIRLDGVPLDEAGRDAKRRIGYLPENNPLYAEMLVSEYLDFV"
BASE COUNT          289 a          666 c          597 g          329 t
ORIGIN      
        1 agggatgctg agtgccgtcg taccactggt gcgtgccggc gaccccgtcg tactcgaact
       61 tgttgccgac ggcctcccag gtgttgccgc cgtcgttcga gcgctggacc acctggccga
      121 accacccgct cgtctgcgac gcgtacagcc ggtcaggatc ggcaggggaa cccttgaggt
      181 ggtagatctc ccagcccgga aagtgcgggc cgctcacctg ccaccggtcc cgcttgccgt
      241 ccgccgtcag gacgaacgcg cccttccgcg tccccaccag tacccgtacc ccgctcatca
      301 gtcgctcctt ggctcggcgt cctcgattag tctcgccccg cccccgtcgc ccggcaatgc
      361 cgtgaggcga gccgttactt gatggcgccc ggcttgccga tgacccggcg aaggaccgcg
      421 gtctggcccg tgtggtacgc ctggtggaac atgatcgtgg ccagcagcga cccgaccgtc
      481 tcgtccggat tgttggtcgg gctgccgggc gccggctggg ccagcgtctc caccgggaac
      541 ccggccagcc catcgtccat ccgccggctc gagtcgtccc acgcggccac cagctggccg
      601 aagtcgaccg cccgggatgg atcggtaagc ggcgggccgc cccgggcgta ctgggagagg
      661 tgatccttat ccagcatggg cgtctgccgc accagcggca gcgcgcccgc gtaggcccaa
      721 accaggtgac ccatgatcca gttgaggctg ttgccgccga gctccggctg gagcaggctc
      781 tcctcgtggg tgatcccttc cacgttgcgg cgcacgaccc ggtgcaccag ctgcgcctgg
      841 taacggatcg cctggatcgc gccgtcgcgc tcggtcgtca tcgcgctcat gattccctcc
      901 gggtgacgtt attacgatat cgtagcatct ttgccaagta tggcccccgg catcgatgac
      961 gtcaaccccg gccctcgcgc ccgcggagga tggcgtatat tccgacatcg tagcaattcg
     1021 atccttcggg agtcggcatg gcgctggatt tcctgtctcc actgcacaag gcctcgcgcc
     1081 agatctcggt ctatctcgag acccggacgc gggaactcgg tgtgagcccc ctcgagggcc
     1141 atgtcctgac ctacctcagg agctatgcgc cggcctcgat tcgggacctg gtgcgggtgt
     1201 tcggcgtcaa gcagtccacc ttcaccagcc tgctcgaccg cctcgagcgc gccggctacg
     1261 cgcaacggac gatcaatccg gacgatcggc gctcctttct ggtgcacatc accgacaagg
     1321 ggagcgagct ggcctcccgg atcacccggc tgctgcgaac cctggaggac gacatccgcg
     1381 agcgggttga gggacgggac gtgaaggggt ttcaggcggt catgcaggca gtggaggaga
     1441 tcacccacgt gcgcttgcgg gaccgctgac gatcccgcga ctttcccggt accccgggca
     1501 tcagaggccg ggcgtcactc gctaaaccga ccggcatggg acaaactgtc accgtccggg
     1561 ccaccacaac ctcactgccc atgtctctac tcgcccttga cggggtgtcg aagcggttcg
     1621 gcggcatcgc cgccgtcgat caggtctcct tccaggtgga tcgcgggcag gtggtcggct
     1681 ttctcggccc caacggggcg ggcaagtcga ccaccatgcg catgatcacc cagttcatcg
     1741 agcccgacgc cgggtccatc cgactcgatg gcgtcccgct cgatgaggcc ggccgcgacg
     1801 ccaagcgccg gatcggctac ctccccgaga acaatccgct gtacgccgag atgctggtct
     1861 cggagtacct cgacttcgtg g
//
LOCUS       DASVOD010000456         3884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_812862, whole genome shotgun sequence.
ACCESSION   DASVOD010000456 DASVOD010000000
VERSION     DASVOD010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812862"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VF720_10350"
     CDS             complement(<1..1051)
                     /locus_tag="VF720_10350"
                     /inference="COORDINATES: protein motif:HMM:NF040068.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879801.1"
                     /translation="MTLFESVAARSLLPLVAKPGRYRGPLTSAPPDRFDRSRLRVAII
                     HPLHMESGLGDLVTRGAFRAIESIAGAAADLAFLPWTDLDERLAAGNRDYWGYESGRP
                     LSGFDVLLFTPPSGLSFVEIPRMLRRAGLAPMAADRRGDVPVVGVIGSLMANPLPLAD
                     IADWILFGEPEGVLPLLMTRLVAAAGASSRTPRAALLAAAAGCPGTWSTSGAFDACVP
                     VLAGDDRLLLDEPGICPVVEAAAEGCHLDIRRAAGHERFRFSWRPAAWAWTRPADDAM
                     ARVDAVLKETGVDRVDLSGDDAWRHPDLASIVESINRRFPGVRIALDDWGVGALKPWL
                     AREIFRGRRGPVTLSP"
     gene            complement(1226..1963)
                     /locus_tag="VF720_10355"
     CDS             complement(1226..1963)
                     /locus_tag="VF720_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX7879802.1"
                     /translation="MSISHLFNDIDSFFFPTACYLCSTPVSRRRQLLLCASCRREFIP
                     TPGACCLLCRAERRDSEGFTAGRDCSVRAHANFRVLAAVQMVAPADRLVHALKFHDRP
                     EMGVTMALLMARRLASAQARNWDLVAPLPLHRTRERERGYNQGLILAEPLARLLKAEL
                     EPRLLTRTRSTARQADLDHRARAANVAGAFRTRERRQAALASRIGERRVLLVDDVATT
                     GHTLVAAVSAVLEGGARSAGAVVFALA"
     gene            complement(2056..3291)
                     /gene="rodA"
                     /locus_tag="VF720_10360"
     CDS             complement(2056..3291)
                     /gene="rodA"
                     /locus_tag="VF720_10360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX7879803.1"
                     /translation="MTTARTRIGGRTFDWQILLIVLALVAFGVAMVYSATHIPSSPGR
                     ANLWRSQILWAMLGLTVATIGALVPYRVFETFAHILYAGSVFLLLLVPVIGVSEYGAK
                     RWLDFGGFNFQPSEPAKILTVLMLARYLDRKRLDLTRPAEVMGAVGIMLLPWFLIVRQ
                     PDLGTSLSLPVAAAAMLYWAGLPAYILLLTATPLISMITSLWMWIWVPYILALFGAMR
                     LFRAPRLMMVAVLLVNIGLGLATPHIWNHLKPYQRERVHTFLDPNRDRSGSGYQIIQS
                     RIAIGSGGAFGKGYMEGTQKALAFLPMQHTDFIYSVVGEEFGFIGTTGVLLLLGGLII
                     RGFWVAGRARNRFASLLAVGIATTFLFHTLVNTAMTAGMAPVTGIPLPFISYGGTFMI
                     TCLFQAGLLLNVAIRRNEY"
     gene            complement(3288..>3884)
                     /locus_tag="VF720_10365"
     CDS             complement(3288..>3884)
                     /locus_tag="VF720_10365"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7879804.1"
                     /translation="EKRRKARPGTGPALNLSIGQGEILMTPVQIASMAATMANGKVPR
                     LHLVREMSDEPGFEPYVADTTARGVVPVSAGVLEYARRGMWRVVNGGEGTGKRAAVDS
                     VVVAGKTGSAQHSGEGPTHALFICFAPYENPTIAIAVVLEARGGGGAFAAPVAHRVLD
                     HYFHPDRYKPAPDSLAVVDTVVVAVSTAPSDSIFPEGD"
BASE COUNT          695 a         1286 c         1370 g          533 t
ORIGIN      
        1 agggcgagag ggtgaccgga ccgcgacggc cacggaagat ctcgcgggcc agccacggct
       61 tgagcgcgcc cacgccccag tcgtcgaggg cgatgcgcac gccgggaaat cgccgattga
      121 tcgactcgac gatcgaggcg agatcgggat gtcgccaggc atcatccccg gacaggtcga
      181 cgcggtcgac gccggtttcc ttgagcacgg catcgacgcg cgccatggcg tcgtccgccg
      241 ggcgcgtcca cgcccaggcg gcggggcgcc acgagaagcg gaagcgttcg tgcccggcgg
      301 cgcggcggat atcgagatgg cacccttcgg ccgccgcctc caccacgggg cagatcccgg
      361 gttcatccag caggaggcgg tcatcgccgg ccagtacagg gacacacgca tcgaacgctc
      421 ccgacgtgct ccaggtaccc gggcatccgg ctgcggccgc gagaagcgcg gcgcgcggcg
      481 tgcgcgaaga ggcgccggca gccgcgacca accgtgtcat gagtagcgga agcacccctt
      541 ccggttcgcc gaacaggatc cagtcggcga tgtcggccag aggaagcgga ttggccatca
      601 gtgaaccgat gacgcccacg accggaacgt cgccccggcg atcggccgcc atcggcgcga
      661 gccccgcgcg acgcagcatg cgcgggattt ccacgaagga gagcccgctc ggcggcgtga
      721 atagcaggac gtcgaacccg gagagcggac ggcccgattc gtatccccag tagtcccggt
      781 tgcccgcggc gaggcgctca tcgagatcgg tccacgggag gaacgcaaga tcggccgccg
      841 cgccggcgat cgactcgatg gcgcggaacg caccacgggt gaccagatcg ccgaggcccg
      901 actccatgtg cagcgggtgg atgatcgcca cgcggaggcg tgatcggtcg aaacgatcgg
      961 ggggcgcgga cgtgagcggg ccccggtacc gacccggctt ggcgacaagc ggaagaagcg
     1021 agcgtgccgc gacgctctcg aacagagtca tgtgcgtttc gaagtcctat gacgagcggc
     1081 ccggcttcgg gaacgcgatg acttggcgtc agtccccgcc ggcaacggcc gcaagcgttt
     1141 gtaactggag ggccggcggg cgataacccc gctcgttcca agcgcttgaa gctatcacac
     1201 ccctcccctg cactcaacgg ccggctcagg ccagggcgaa gacgaccgcc ccggccgatc
     1261 gggcgccacc ttcgagcacg gccgagaccg cggccaccag tgtgtggccg gtggtggcga
     1321 cgtcgtcgac gaggagcacg cgccgttctc cgatccggct cgccagcgcg gcctgcctcc
     1381 gttcccgggt ccgaaacgca cccgccacgt tggcggcccg cgcccggtga tcgaggtcgg
     1441 cctgcctggc cgtggaccgc gttcgtgtca ggagccgcgg ttccagttcc gctttcagca
     1501 atcgggccag cggttccgcc aggatcagcc cctggttgta gccccgctcg cgttcgcggg
     1561 tccgatgaag cggcagtggc gcgaccaggt cccagttccg tgcctgcgcc gacgccagcc
     1621 gtcgtgccat caggagggcc atcgtgaccc ccatctccgg ccgatcatga aacttgagcg
     1681 catgcaccag ccgatccgcc ggcgccacca tctgaaccgc cgccagcacc cgaaagttgg
     1741 catgcgcccg gacggaacag tcacgaccgg ccgtgaatcc ctcagagtca cgccgttcgg
     1801 cgcggcagag gagacagcag gccccgggtg tcggaatgaa ttcccgtcgg caggaggcgc
     1861 agagcagaag ctgccgtctc cttgagacgg gcgtggaaca gaggtagcag gccgtgggaa
     1921 aaaagaaact gtcgatgtcg ttgaatagat ggctgatgga catgcggggt cctccgtagt
     1981 ggaggacggg tgatcgaggt tagatcaatc gtgcggggag tggccagagc cccgcggggc
     2041 gccagcgccg cgacattaat actcgttccg ccggatggcc acattcagca acaaccccgc
     2101 ctggaagagg caggtgatca tgaacgtgcc gccgtagctg atgaacggca gggggatgcc
     2161 ggtgacgggg gccatgccgg cggtcatggc cgtgttgacc agcgtatgga acaggaacgt
     2221 cgtggcgatg ccgacggcca ggagactggc gaagcggttg cgggcccgcc cggccaccca
     2281 gaagccccgg atgatcagtc cgccgagcag gagaaggacg ccggtggtgc cgatgaaccc
     2341 gaattcctcc cccaccaccg agtagatgaa gtcggtgtgc tgcatcggca ggaacgcgag
     2401 ggccttctgc gttccctcca tgtacccctt tccgaacgca cctcccgagc cgatggcgat
     2461 gcgcgactgg atgatctggt agcccgatcc ggagcggtcg cggttggggt cgaggaacgt
     2521 gtggacacgc tcgcgctggt agggcttcag gtggttccag atgtggggcg tggccaggcc
     2581 cagcccgatg ttgacgagga gcaccgccac catcatgagg cgcggagccc ggaagaggcg
     2641 catcgccccg aaaagcgcca ggatgtacgg cacccagatc cacatccaga gcgaggtgat
     2701 catgctgatg agcggcgtgg cggtcaaaag gaggatgtac gccggcaggc cggcccagta
     2761 gagcatggcg gccgccgcca ccggcagcga aagcgaggta ccgagatcgg gctggcgcac
     2821 gatgagaaac cacgggagga gcatgatgcc cacggccccc atcacctcgg ccgggcgcgt
     2881 caggtcgagc ctctttcggt cgaggtagcg ggccagcatc agcacggtga ggatcttggc
     2941 cggctccgac ggctggaaat tgaagccgcc gaagtccagc catcgcttgg cgccgtactc
     3001 cgacacgccg atcaccggca cgagcagcaa cagaaagacg ctcccggcat acaggatgtg
     3061 cgcgaacgtc tcgaagacgc ggtacgggac gagcgcgccg atggtggcca ccgtgagccc
     3121 gagcatcgcc cacaggatct gtgagcgcca caggttggcc cgccccgggg aggagggaat
     3181 gtgcgtggcg ctgtagacca tggccacgcc gaatgccacg agtgccagca cgatcagcag
     3241 gatctgccag tcgaaggtcc gcccgccgat gcgggtccgg gcggtggtca atccccctcc
     3301 gggaagatcg agtcggacgg tgcggtgctc acggccacga ccaccgtgtc caccacggcc
     3361 agcgagtcgg gcgcaggctt gtatcgatcg ggatggaagt agtggtcgag cacgcggtgc
     3421 gccaccggcg ccgcgaaggc gcctccgccg ccgcgggcct ccagcacgac ggcgatggcg
     3481 atcgtcgggt tctcgtacgg cgcgaagcag atgaagagcg cgtgagtggg gccctcgccg
     3541 gaatgctggg ccgagccggt cttgccggcc accaccacgc tgtcgaccgc ggcgcgcttg
     3601 ccggttcctt cgccgccgtt caccacccgc cacatgccgc gccgggcgta ctcgaggacg
     3661 ccggcgctca ccggcaccac gccgcgggcc gtggtatcgg ccacgtaggg ctcgaagccg
     3721 ggctcatcgg acatttcccg gaccaggtgg agccgcggca ccttgccgtt ggccatcgtg
     3781 gcggccatcg aggcgatctg gaccggcgtc atgaggattt caccctgacc gatcgacagg
     3841 ttgagcgccg gcccggtgcc cggcctggct ttccggcgct tttc
//
LOCUS       DASVOD010000457         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_291644, whole genome shotgun sequence.
ACCESSION   DASVOD010000457 DASVOD010000000
VERSION     DASVOD010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291644"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1620
                     /gene="ligD"
                     /locus_tag="VF720_10370"
     CDS             <1..1620
                     /gene="ligD"
                     /locus_tag="VF720_10370"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX7879805.1"
                     /translation="DSPLRFSDHVDGAGLDTWKEACRLKLEGIVSKRRDAPYRPGRGN
                     DWQKSKCQSRQEFIIGGYTDPTGGRVGFGALLIGVRDASGKLKPAGRVGTGFDTAGLA
                     DLHRRLKALEIDTSPFDPSPVGARARGVHWVKPILVGEVEFTEWTSEGQLRHPSFQGL
                     REDKKAAEVTRERPVHQREAAGTKTSAGRIRRAAERVTATRKSADPSRGGAKKSVPAK
                     KSVAPKSSPSRKTAPKKAASKTRPEPSIADVTITHPDRVVYDSMGITKADLAAYYEAV
                     AAHMLPHMSHRPLALVRCPGGEGKGCFYQKHLGPGVPKAIRAITIEETSTTGDYPWVN
                     NAAGLVGLVQMGVLEIHPWGSRVDDIEKADRLIFDIDPDPKLAWARVAALAFRLKDRL
                     EELGLESWLKTTGGKGVHVVVPIQRRYEWPVVKGFCQAIAQEMARRYPDEVTSVMSKA
                     KRVGRIFLDYLRNGRGATAIAAYSTRARAGAPVATPIAWDELARIGRGDAFNVRTLPK
                     RLALLTHDPWAKLAASRQGLSQKMLAGLAID"
     gene            1673..3259
                     /locus_tag="VF720_10375"
     CDS             1673..3259
                     /locus_tag="VF720_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7879806.1"
                     /translation="MRFGTRLSLAMVAPIVLLLVGYGYLDEQRTRAQFREELRREGRT
                     IIRTVKLALEDALRDRNISDVHQLIDQITGFERVYGLRLFDPDGHMAYQSHVLEGESF
                     RSAGPLAAVLATRQPFESERPIGNQTVLTYIVPLVSPDGKLYGAVQLLQLRSFIDDEV
                     RVARRSIVWLTLAMILVAGLVIIVVTRLVVDRPLGEFIDKLKAIGPIDRAERIPVKGG
                     DELKRLAREFNLLWERLAQSHRSMVTAQEERRLAEMRLKNAERLAALGELAAGLAHQI
                     GTPLNVIGGRADALRRRHQSEPHLDKNLQIISEQINRIARIVREMLQFAQVKALNPVP
                     VDVDELVGSVLEFLEPRLEQDHINVHRRLPSGLPHVPADQDQLYEVFLNLMINAIDSM
                     SGGGELTVTGTRGPKDHPETAGIARDVVSITIADTGAGIAPEDLGRIFQPFFTTKGVG
                     RGTGLGLSVAYGIVREHGGWIDVTSDSGRGAALTVHLPVHPPEDGGGGNGAPDNGAPD
                     NGASDNGASANRVPANGASA"
     gene            3256..>3610
                     /locus_tag="VF720_10380"
     CDS             3256..>3610
                     /locus_tag="VF720_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7879807.1"
                     /translation="MNSTPRILVVEDDTAMRDLLADELREAGFAIVTAADGNEALRQL
                     DRHEVNAVVTDLQMPGLTGDQLLEAIRTRDSRLPVVIITAFGTIDTAVKTIKAGAFHF
                     LPKPFPMHQLVATLEE"
BASE COUNT          638 a         1286 c         1145 g          541 t
ORIGIN      
        1 gactccccgc ttcggttcag cgaccacgtc gacggcgccg gcctcgacac ctggaaggaa
       61 gcctgccgct tgaaactcga aggcatcgtg agcaagaggc gcgacgcgcc gtaccgcccc
      121 ggccgcggaa acgactggca gaagtccaaa tgccagtcgc gccaggagtt catcatcggc
      181 ggttataccg atcccaccgg cggccgggtg ggtttcggcg ccctcctcat cggcgttcgt
      241 gatgcctccg gcaagctcaa gcccgccggc cgcgtcggca ccggtttcga taccgccggg
      301 ctcgccgatc tccacaggcg cctcaaggcg ctcgaaatcg acacctcccc cttcgatccc
      361 agcccggtcg gcgcgcgcgc gcgcggcgtg cactgggtca agccgatcct cgtcggcgaa
      421 gtggagttca ccgagtggac atccgaagga cagctccgcc acccgagctt ccagggattg
      481 cgcgaggaca agaaggcggc cgaagtcacg cgggagcggc cggttcatca gcgcgaagcc
      541 gccggcacga agacgagtgc cggccgaatt cgccgcgcgg ccgagcgcgt gacggcgacg
      601 cggaagagcg cggatccgtc gaggggcggc gcgaagaaat ccgtgcccgc gaaaaagtcc
      661 gtagccccga aatcatcccc aagccgcaag acggcaccga agaaggccgc cagcaagacc
      721 cgccccgaac catccatcgc cgacgtcacc atcacccatc ccgaccgggt cgtctacgat
      781 tcgatgggaa tcacgaaggc cgatctcgcc gcgtactatg aagcggtggc cgcccatatg
      841 ctgccgcaca tgtcgcatcg cccgctggcc ctggtgcgat gtccgggcgg cgagggaaag
      901 gggtgcttct accagaagca tctcggcccg ggcgtgccga aggccatccg ggccatcacg
      961 atcgaggaaa cctccaccac cggcgattat ccgtgggtca acaacgcggc gggactcgtc
     1021 ggccttgtgc agatgggcgt gctcgaaatc catccatggg gatcgcgggt ggatgatatc
     1081 gagaaggccg atcgcctcat cttcgacatc gacccggatc cgaaacttgc ctgggcgcgg
     1141 gtggcggcgc tggcgtttcg cctcaaggat cgcctcgagg aattgggtct cgaatcgtgg
     1201 ctgaagacga cgggcggcaa gggcgttcac gtcgtcgtgc cgatccagcg ccgttacgag
     1261 tggccggtgg tgaaggggtt ctgccaggcc atcgcgcagg aaatggcgcg gcgctacccg
     1321 gacgaggtga ccagcgtgat gtccaaggca aaacgtgtcg gaaggatctt tctcgactac
     1381 cttcgcaacg gccgcggcgc caccgccatc gcggcctatt ccacccgcgc gcgcgccggt
     1441 gcgccggtgg ccacgccgat cgcctgggac gaactggcgc ggatcggccg gggcgatgcg
     1501 ttcaacgtca ggacgctgcc gaaacggctg gccttgctca cgcatgaccc atgggccaaa
     1561 ctggccgcct cccggcaggg gctgagccag aaaatgctgg ccggcctggc catcgactga
     1621 cgctcctttc cgcgaggtgc cgcataagtc aggttgtgca cgccgctgat cgatgaggtt
     1681 cggcacccgt ctctcgctcg ccatggtggc gccgatcgtc ctgttgctcg tcggctacgg
     1741 ctacctggac gagcagcgca cgcgcgccca gttccgcgag gagctccgcc gcgaggggcg
     1801 caccatcatc cgcacggtca agctggcgct cgaggatgcc ctgcgcgacc gcaacatctc
     1861 cgacgtgcac cagctcatcg accagatcac cggcttcgaa cgcgtgtacg gcctgcgcct
     1921 cttcgatccc gacgggcaca tggcgtacca gtctcacgtt cttgaggggg agtcgttccg
     1981 ttcggccggg ccgctcgccg ccgtcctggc cacgcgtcaa cccttcgaga gcgagcgccc
     2041 catcggcaac cagacggtgc tcacctacat cgtgcctctg gtgtcgccgg atggaaagct
     2101 ttacggcgcg gtgcagctcc tgcagctgcg atccttcatc gacgacgaag tgcgtgtggc
     2161 ccgccgcagc atcgtgtggc tcacgctggc catgatcctg gtggccggcc tcgtcatcat
     2221 cgtggtgaca cggctcgtgg tcgaccggcc gctcggcgag ttcatcgaca agctgaaggc
     2281 catcggaccg atcgaccggg ccgagcgcat tccggtcaag ggtggcgatg aactgaagcg
     2341 cctggcccgc gagttcaacc tcctgtggga acgcctggcg caatcgcatc gctcgatggt
     2401 gacggcccag gaagagcgcc gcctggccga gatgcgcctg aagaacgccg agcgcctggc
     2461 cgccctcggc gagctggcgg ccggcctggc ccaccagatc ggaaccccgc tcaatgtcat
     2521 cggcggtcgg gccgacgcgc tccggcgccg ccaccagtcg gagccccatc tcgacaagaa
     2581 tctccagatc atctcggagc agatcaaccg cattgcgcga atcgtgcgtg aaatgctcca
     2641 attcgcccag gtgaaggcac tgaacccggt gccggtggac gtggacgagc tggtcggttc
     2701 cgtcctcgag ttcctcgagc cccggctcga gcaggaccac atcaacgtgc atcgccggct
     2761 cccgtcgggg cttccccacg tgccggccga ccaggaccag ctctacgagg tctttctcaa
     2821 cctgatgatc aacgcgatcg attccatgtc gggcggcggc gaactcaccg tgaccggcac
     2881 ccggggcccg aaagaccatc ccgaaaccgc cggcatcgcc cgcgacgtcg tctccatcac
     2941 catcgccgat accggcgccg gcatcgcgcc cgaagacctg gggcgcatct tccagccttt
     3001 cttcaccacc aagggggtgg ggcgcggcac cggcctcggc ctctcggtgg cctatggcat
     3061 cgtgcgcgag cacggcggct ggatcgacgt gaccagcgac tccggccgcg gcgccgcgct
     3121 caccgtgcac ctgccggtcc atccgccgga ggacggcgga ggcggaaacg gcgcacccga
     3181 caacggcgca cccgacaacg gcgcatccga caacggcgca tccgcgaacc gcgtacccgc
     3241 gaacggagcc tcggcatgaa ttccaccccc cgcatcctcg tcgtcgaaga cgacaccgcc
     3301 atgcgcgacc tcctggccga cgaactccgc gaggccggct tcgccatcgt caccgcggcc
     3361 gacggcaacg aggcgctcag gcaactcgat cgccacgagg tcaacgccgt cgtcaccgat
     3421 ctccagatgc ccggcctcac cggcgatcag ctcctcgaag ccatccgcac gcgcgacagc
     3481 cgcctgccgg tcgtcatcat cacggccttc ggcaccatcg ataccgccgt caagacgatc
     3541 aaggccggcg cgttccattt cctccccaag ccattcccga tgcaccagct cgtggccacg
     3601 ctcgaggaag
//
LOCUS       DASVOD010000458         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_135520, whole genome shotgun sequence.
ACCESSION   DASVOD010000458 DASVOD010000000
VERSION     DASVOD010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135520"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VF720_10385"
     CDS             complement(<1..663)
                     /locus_tag="VF720_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEX7879808.1"
                     /translation="MNSITIPFIVAGVILAFFAAMSTMLRTYIKVPPNKVAIISGSKR
                     RLADKSTVGFRLVHGGATLVLPGLERVDYLDLNVIPVSLETRKAITKEGVAVNVQAVA
                     NVKIGSDDISLRNSAERFLAMPISGVKEVIIQTLEAHLRAICGTLTVEEINSDRQAFA
                     QRMTAEAAEDFRKMGIVIDVFAVQHISDEHGYLDSLGRKRTAEVVRDAEIGEADAKRD
                     ALQ"
     gene            complement(660..1211)
                     /locus_tag="VF720_10390"
     CDS             complement(660..1211)
                     /locus_tag="VF720_10390"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7879809.1"
                     /translation="MVGFLVIGGVGFTLLVFTWVVGEMFDLGHDLATFVGHEIGTANI
                     EMGGHEMAGPSPFSSRVVFTFLTAFGGGGAVAAACGQPMWASILYGKGAGLTVAASTW
                     LLARLLWRRQSSSTLEVSSLVGCRGRVVVGVGVGALGQVTLLVGGGSSTFLARSAGDA
                     IPAGDSVLVVASEGDTLIVRRDS"
     gene            1449..2372
                     /locus_tag="VF720_10395"
     CDS             1449..2372
                     /locus_tag="VF720_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX7879810.1"
                     /translation="MGPFPAEPIATFSIVAYDPATGDLGVAVQSKFFAVGSVVPWAKA
                     GVGAVATQSFGNTTFGPRGLEMLASGKLPKEVMAALLADDPGKAQRQVGIVDAKGNTA
                     TYTGETCMAWAGGTEGANYSAQGNILAGAPVVAAMGKAFEETKGLLGDRLLAALDAGQ
                     AAGGDSRGMQSAALLVVREKGGYGGFNDRWCDLRVDDAPNPFTELRRLYNLWKPNALV
                     LEGYKACDAKEWEKAFALGKEALALDPKTGDPQYHLGCYYSKAGRGAEAIEWLRQSVA
                     LDPGHGKTALQDSDYAPLRDNPDFKKLTSGD"
     gene            2373..3359
                     /gene="trxB"
                     /locus_tag="VF720_10400"
     CDS             2373..3359
                     /gene="trxB"
                     /locus_tag="VF720_10400"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX7879811.1"
                     /translation="MRHRLVIIGSGPAAHTAAIYAARASLKPVLFEGFLAGGVPAGGQ
                     LTTTTDVENYPGFPEGIDGSELMVRMRQQSLRFGTEIHTETVNAVDLSKHPFGVTTDT
                     HHLEADAIIVATGATAKRMMVPGEEKLWQNGISACAVCDGALPIFRNKALAVIGGGDT
                     ACEEASHLAKFGSKVYLIHRRDELRASKIMQKRVFDNPKIEMVWNSVLESAHGDDLLQ
                     SIRVRNVQTDESRTLEVSGLFYAIGHVPNTAFLSGQLALHDNGYVRTKPGTTETSVDG
                     VFAAGDVQDHKYRQAVTAAGSGCMAALEAESWLAMHEGADMQLKYLAIEDGE"
     gene            3394..>3767
                     /locus_tag="VF720_10405"
     CDS             3394..>3767
                     /locus_tag="VF720_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879812.1"
                     /translation="MSKAAPTRIPVVAILLVLAVSVIAMAPAIFLGKVPAQSDWLPLY
                     GPWGDKAWSADAAPVANRGGGDSFFVYLPDRLAAVAEWKKGRLPLWNPHVSAGIPFLG
                     MQTANPLDPLILLEILLPTGYG"
BASE COUNT          649 a         1244 c         1281 g          593 t
ORIGIN      
        1 ctggagagcg tcgcgcttgg cgtcggcctc gccgatctcg gcatcgcgca cgacttcggc
       61 ggtgcgcttc cggcccagcg agtcgagata gccgtgctcg tcggagatgt gctgcacggc
      121 gaagacgtcg atgacgatcc ccatcttgcg gaagtcttcg gcggcctcgg ccgtcatccg
      181 ctgggcgaaa gcctggcggt cggagttgat ctcctcgacg gtgagcgtgc cgcagatggc
      241 acggaggtgg gcctcgagcg tctggatgat gacttccttg acgccgctga tgggcatggc
      301 gaggaagcgc tcggccgagt tgcgcaagga gatgtcgtcc gaaccgatct tgacgttggc
      361 caccgcctgc acgttgacgg ccacgccttc cttcgtgatg gccttgcgcg tctccaggct
      421 caccgggatg acgttgaggt cgaggtagtc gacgcgttcg aggccgggga ggaccagcgt
      481 ggcgccgccg tgcacgagcc ggaaaccgac ggttgacttg tccgcgagcc gccgcttgga
      541 gccggagatg atggccacct tgttgggcgg caccttgatg taggtgcgga gcatcgtgct
      601 catggcggca aagaaggcca ggatgacgcc ggccacgatg aagggaatgg tgatcgaatt
      661 catgagtctc tcctgacgat cagggtgtcg ccctcactgg ccacgacgag gacggagtcg
      721 ccggccggga tggcatcgcc cgcggaacgg gcgagaaacg tgctggaacc gcctcccacg
      781 aggagcgtca cctgaccgag cgctccaacg cccaccccca cgaccacgcg acccctgcac
      841 cccacgaggc tcgagacctc gagggtgctc gaggactggc gacgccagag gagccgggcc
      901 aggagccatg tggatgccgc cacggtcagg ccggcacctt ttccgtagag gatcgaagcc
      961 cacatcggct ggccacatgc ggccgccacg gccccgccac cgccgaacgc ggtgagaaag
     1021 gtgaagacca cgcgcgagga gaagggggag ggaccggcca tttcatgccc gcccatctcg
     1081 atgttggccg tgccgatctc atgcccgacg aatgtggcca ggtcatgtcc caggtcgaac
     1141 atctcgccga cgacccaggt gaagacgagg agcgtgaagc cgactccgcc gatgacgagg
     1201 aagccgacca tggatacctc cggggtgagg gacggtcgtg ggtgccgctc cggcatccat
     1261 gcgggcccta cgtggcatac gcccggatgt cgcgcggtgg gtgatatagt cccccggccc
     1321 cgtcgaaggg tcccccgaac cggagaaccg acatgccgtt gtccacgctc attcgcgact
     1381 ctttccgcat gggaatcatc acgatggctg ccatccgcct cgtcctggcc gcgcccgctc
     1441 atgcctcgat gggccctttc cccgccgagc cgatcgccac gttttcgatc gtggcgtacg
     1501 accccgccac cggagacctg ggcgtggccg tgcaatcgaa gtttttcgcg gtgggctcgg
     1561 tggtgccgtg ggcaaaggcc ggcgtgggcg ccgtggccac ccagtcgttc ggcaacacca
     1621 cgttcggtcc gcgggggctc gagatgctgg cctcgggcaa gttgccgaaa gaggtgatgg
     1681 cggccctgct tgccgacgac ccggggaagg cccagcgcca ggtcggcatc gtcgacgcga
     1741 agggaaacac cgccacgtac accggcgaga cctgcatggc ctgggccggc ggcacggaag
     1801 gcgcgaacta cagcgcgcag gggaacatcc tggccggggc cccggtcgtg gcggccatgg
     1861 ggaaggcctt cgaggaaaca aaggggctcc tcggcgatcg gctgctggcg gccctcgatg
     1921 ccggccaggc cgccggcggg gacagccgcg gcatgcagtc ggccgcgctc cttgtcgtgc
     1981 gcgagaaggg gggttacggc ggtttcaacg accgctggtg cgacctccgg gtggacgacg
     2041 ccccgaatcc ttttacggag ctgcggcgtc tatacaatct ctggaagccc aacgcgctcg
     2101 tgctcgaggg gtacaaagcc tgcgacgcga aggagtggga gaaggcgttc gcactgggca
     2161 aggaggccct ggcgctggat ccgaagaccg gcgatccgca gtatcacctc gggtgctact
     2221 actcgaaggc cgggcgcggc gcggaggcca tcgagtggct ccgtcagtcg gtggcgctcg
     2281 accccggcca cggcaagacg gcgctgcaag acagtgacta cgcgcccctg cgcgacaatc
     2341 ccgacttcaa gaaactgact tcaggagact agatgcgtca ccgcctcgtc atcatcggtt
     2401 ccgggcccgc cgcgcacacc gcggccatct acgcggcccg cgcgtcgctc aagcctgtcc
     2461 tgttcgaggg attcctggcg ggaggcgtgc cggcgggcgg ccagctcacc accacgacgg
     2521 acgtcgagaa ctatcccggc ttcccggagg ggatcgacgg cagtgagctg atggtgcgca
     2581 tgcgccagca gtcgctgcgc ttcggcaccg agatccacac cgagacggtg aatgccgtgg
     2641 acctctcgaa gcaccctttc ggcgtgacga ccgacacgca ccatctcgag gccgacgcca
     2701 tcatcgtggc caccggcgcc accgcgaaac ggatgatggt gcccggtgag gagaagctct
     2761 ggcagaacgg aatttccgcc tgcgccgtct gcgacggggc ccttcccatc ttccgcaaca
     2821 aggcgctcgc cgtcatcggc ggcggcgata cggcctgcga ggaagccagc cacctggcca
     2881 agttcggcag caaggtgtac ctgatccacc gccgcgacga attgcgcgcc agcaagatca
     2941 tgcagaagcg cgtcttcgac aacccgaaga tcgagatggt ctggaacagc gtcctcgaat
     3001 cggcccacgg cgacgatctc ctccagtcga tccgtgtccg caacgtccag accgacgaat
     3061 cgcgcacgct ggaagtgagc ggactcttct acgccatcgg ccatgtcccc aacaccgcgt
     3121 tcctgtcagg gcagctcgcg ctgcatgaca acggctacgt ccgcaccaag cccggcacga
     3181 ccgagaccag cgtggatggt gtcttcgccg ccggcgatgt gcaggaccac aagtatcgcc
     3241 aggccgtgac ggccgccggc tcgggttgca tggccgcgct ggaggcggaa tcgtggctgg
     3301 ccatgcacga gggcgcggac atgcagctca agtacctggc gatcgaggac ggggagtagg
     3361 cttggctgcc gcgacaggcg atcgcgactg aggatgtcga aggccgcgcc gacccgcatt
     3421 cccgtcgtcg cgatcctcct ggttctggct gtctccgtca tcgcgatggc gcccgccatc
     3481 ttcctcggga aagtccccgc gcagtcggac tggctgccgc tctatggacc atggggtgac
     3541 aaggcctggt cggccgacgc cgcgccggtg gccaaccggg ggggtggtga ttccttcttc
     3601 gtctacctgc cggatcgcct tgcggccgtc gccgaatgga agaagggccg gcttccgttg
     3661 tggaatcccc atgtctcggc gggcattccg ttcctgggaa tgcagacggc caacccgctc
     3721 gatccgctga tcctcctcga gatcctcctg ccgaccggct acgggca
//
LOCUS       DASVOD010000459         3553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_864880, whole genome shotgun sequence.
ACCESSION   DASVOD010000459 DASVOD010000000
VERSION     DASVOD010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864880"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF720_10410"
     CDS             complement(<1..435)
                     /locus_tag="VF720_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879813.1"
                     /translation="MSRHPEIDLSRIRSRPARERATRVDLEKLGSPVSPADATILDRL
                     PDLLGAASLKAVVAATARAVRQRKPILLMAGGHVIKTGCAVPILQLIERGVLTAVAFN
                     GAAAIHDFEIALYGATSEDVEAELATGSFGMADETVERMNRVT"
     gene            complement(435..1319)
                     /locus_tag="VF720_10415"
     CDS             complement(435..1319)
                     /locus_tag="VF720_10415"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX7879814.1"
                     /translation="MIRRGWRLAALFSLPIVLWGAAPPVDDSDPLPPPVSETLLRELP
                     ATSDMRITFATGEELTYAIKFGPIRAGTAVMSVVGEEWANGGKCWRLRSTIKSSGFFS
                     GIFKVDDLTESWFDMKGLFSRRFTRLVNEGSYHRHDAVQIDSARNLAFYHPKGDSVAV
                     PPRTQDVLSSIYFARGLPLDVGQSMQIPMHVDRKNAPVELRVLKRETVRVPAGTYACG
                     VVEPILLEPGLFRNEGRVTLWVSDDANRVPVIIKTKVKVGSITAVLESHRHGTPSTGA
                     QPPAPASAEASDTGRRGG"
     gene            complement(1386..1817)
                     /gene="dut"
                     /locus_tag="VF720_10420"
     CDS             complement(1386..1817)
                     /gene="dut"
                     /locus_tag="VF720_10420"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX7879815.1"
                     /translation="MEFVRLDPQAAPPEKMTDGAAGYDLRACLAEPINLAAGGRALVP
                     TGLSLAIPAGFEGQIRPRSGLALKHGLTVLNAPGTIDSDYRGPVGIVLVNLGEASCRI
                     EHGDRIAQIVIAPVVRIEFLERTAIGATERGPGGFGHTGRS"
     gene            complement(1850..3130)
                     /locus_tag="VF720_10425"
     CDS             complement(1850..3130)
                     /locus_tag="VF720_10425"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7879816.1"
                     /translation="MSNVRFEQTALPGGAVLVSEHVPWARSLALGFWFRAGSRDESLV
                     EDGMAHFVEHLAFKGTTKRDALEIARSLEAVGGSLDAFTGRELTCYYSRVLSESLPLA
                     IDVLTDLIGRPTFLEDHVEREKSVVIEEIRGLEDSPEELIHGLVAARMWRGHPLAGSI
                     LGTETSVGAFNAAGVRDYWDRLYRGPSLIVSLAGGFDIDEVRSLLTESLALSSGTPVQ
                     DRRPPAVMPPALVNWKRDVSQEYLCLAAPTPGFGDQRRYALQILSTVLGGGMSSRLFQ
                     RIREDAGLAYTVYSYSDFCQDTGMFGAFLGVSPEKAGAALEMTFDEMRKLRRDGFTRD
                     ELDSARAQVRGGVLMGLESLSTRMNRLARSLFYHGRYQTVEELLAIFDQLTVADVIEQ
                     TADFLDPERWTLVAYGPAATKTLNVAGWKDVEEP"
     gene            complement(3231..>3553)
                     /locus_tag="VF720_10430"
     CDS             complement(3231..>3553)
                     /locus_tag="VF720_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7879817.1"
                     /translation="GTVKVAARDSAAGERAVQIIRGLTEDPEIGRVYEGTVRRIVNFG
                     AFVEILPNRDGLVHISELDTKRVAKVEDVLAEGDIVKVKVIGIDPEGKVKLSRKAVMV
                     EAGV"
BASE COUNT          603 a         1199 c         1163 g          588 t
ORIGIN      
        1 cgtcacgcgg ttcatccgct cgaccgtctc gtcggccatg ccgaacgagc ccgtcgcgag
       61 ctcggcctcc acgtcctccg aggtggcccc gtagagtgcg atctcgaagt cgtggatggc
      121 ggctgcgccg ttgaaggcca cggccgtcag caccccgcgc tcgatcaact ggagaatggg
      181 cacggcgcag ccggtcttga tgacatgacc gccggccatc aggagaattg gtttgcgctg
      241 acggaccgca cgggccgtgg ctgccacgac cgccttgagg ctcgccgccc cgaggaggtc
      301 cgggagtcga tcgaggatgg tcgcgtcggc cggcgagacc ggcgaaccca gcttttccag
      361 gtcgacgcgg gtggcccgct cgcgggcggg gcggctcctg attcgggaaa ggtctatctc
      421 gggatggcgc gacatcagcc cccccggcgc ccggtatccg aggcttcggc actggccgga
      481 gccggcggct gcgcgccggt cgagggcgtt ccatgtcggt gcgactcgag aacggccgtg
      541 atcgacccca ccttcacctt tgtcttgatg atcaccggaa cacggttggc gtcatcggac
      601 acccacagcg tgacgcgtcc ctcgttccgg aagagccccg gctcgaggag gatcggctcc
      661 accacgccgc aggcgtacgt tcctgccggc acccggacgg tctcgcgctt caacacacgc
      721 agttcgaccg gggcgttctt ccgatcgaca tgcatcggga tctgcatgct ctggccgaca
      781 tcgaggggaa gaccgcgggc aaagtagatc gacgataaca cgtcctgcgt ccgcggcggc
      841 acagccaccg aatcaccctt cggatggtag aaggcgaggt tccgggccga gtcgatctgc
      901 acggcgtcgt gccgatgata gctcccctcg ttcacgagcc gggtgaagcg ccggctgaag
      961 agtcctttca tgtcgaacca cgactcggtc aggtcgtcca ccttgaatat ccccgagaag
     1021 aagccgctcg acttgatcgt gctcctgagg cgccagcatt taccgccgtt ggcccactcc
     1081 tcgccgacga cggacatgac cgcggtgcct gcccggatcg ggccgaactt gatggcgtac
     1141 gtcagctcct cgcccgtggc gaacgtgatg cgcatgtccg aggtggcggg caactcgcgg
     1201 agcagcgtct cggagacggg cggcggcagc ggatccgaat cgtccaccgg gggcgcggcg
     1261 ccccagagga cgatcggcag ggagaagagg gcggcaaggc gccacccacg gcgaatcatc
     1321 acgtcagctc cttccgccgg ccgatgccgg cgatggctct tgtcgaatga ccccggagcg
     1381 accggtcagc ttcgaccggt gtgcccgaag ccccccggcc cgcgttccgt ggcgccgatc
     1441 gccgtccttt ccaggaattc gatgcgcacc accggcgcga tcacgatctg cgcgatccga
     1501 tcgccatgtt cgatccggca ggatgcttcg ccaagattga cgaggacgat tcctaccgga
     1561 cctcgatagt cgctgtcgat ggtgccggga gcgttgagta cggtcagccc gtgtttgagg
     1621 gccaggccac tgcggggacg aatttgtcct tcaaaacccg ccggaatggc cagcgacagg
     1681 cccgtgggaa ccagtgcgcg tccgccggcg gccaggttga tcggttccgc caggcacgcc
     1741 cgaagatcgt acccggccgc cccatcggtc attttttccg gaggggccgc ttgcggatcc
     1801 agccggacaa actcgatgag gacggagttt tctcccgatt ttgtcccgct cacggctcct
     1861 ccacgtcctt ccagcccgcc acgttcaggg ttttcgtggc tgccggcccg taagccacca
     1921 gcgtccagcg ctccggatcg aggaagtcgg ccgtctgctc tatgacgtcg gccacggtca
     1981 gctggtcgaa aatcgccagg agttcctcca ccgtctggta acggccatga tagaagaggc
     2041 tgcgcgccag gcggttcatg cgggtggaga gcgactcaag tcccatgagg acgccaccgc
     2101 gcacctgcgc gcgagccgaa tcgagttcgt cccgggtgaa gccgtcgcgc cggagcttcc
     2161 gcatctcgtc gaaggtcatt tccagcgccg cacccgcttt ttcggggctc acccccagaa
     2221 acgctccgaa catcccggtg tcctggcaga agtcggaata gctgtacacg gtgtaggcca
     2281 gcccggcgtc ctcgcggatc cgctggaaga ggcgcgagga catgccgcct cccagcaccg
     2341 tggagagtat ctgcagcgcg tagcgccgct ggtcaccgaa tccgggcgtc ggggcggcca
     2401 ggcagaggta ttcctgtgaa acgtcgcgct tccagttcac gagcgcggga ggcatgaccg
     2461 ccggtgggcg gcgatcctgc accggtgttc cgctcgagag agccagcgat tccgtgagaa
     2521 gcgagcggac ttcgtcgatg tcgaacccgc cggccagcga cacgatcaac gaggggccgc
     2581 gatagagccg gtcccagtaa tcgcgcacgc cggccgcgtt gaaggcgccc acgctggttt
     2641 cggtgccgag gatcgaaccg gcaaggggat gaccccgcca catccgggcc gccaccaggc
     2701 cgtggatcaa ctcctcgggg gaatcctcca gaccgcggat ctcctcgatc acgaccgact
     2761 tttcgcgctc gacgtgatcc tcgaggaaag taggccggcc gatcaggtcg gtcaggacat
     2821 cgatggccag gggaagactt tcggaaagga cacggctgta gtagcaggtg agctcacggc
     2881 cggtgaaggc atcgaggctc ccccccacgg cctcgaggct ccgcgcgatt tccagggcat
     2941 cgcgcttcgt cgtccccttg aacgccaggt gctcgacgaa gtgggccatg ccgtcctcga
     3001 caagggactc gtcgcgagaa ccggcccgga accagaaacc gagtgccagc gaccgggccc
     3061 agggtacgtg ctcactgacg agcacggcgc cgcccggcag cgcggtctgt tcgaatcgta
     3121 cgttgctcat ggcctcccga agaaaaaagg ccggcgacgc ccggccgggt gaagccaccc
     3181 ggttgccgtc gtcgccggcc tcgaaatccg tttccggatg ctggagtccg tcagacgccc
     3241 gcttccacca tcacggcctt ccgggagagc ttgaccttcc cttccggatc gataccgatg
     3301 accttcacct tcacgatgtc cccttcggcc agcacgtcct cgaccttcgc cacccgcttg
     3361 gtgtcgagct cggaaatgtg gaccaggccg tcgcggttgg gaaggatctc gacgaaggcg
     3421 ccgaagttga cgatgcggcg caccgtcccc tcgtacaccc ggccgatttc ggggtcctcg
     3481 gtgaggccgc ggatgatctg gacggcgcgc tcgccggccg ccgaatcgcg ggcggccacc
     3541 ttgacggtgc cgt
//
LOCUS       DASVOD010000460         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_970209, whole genome shotgun sequence.
ACCESSION   DASVOD010000460 DASVOD010000000
VERSION     DASVOD010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970209"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VF720_10435"
     CDS             <1..700
                     /locus_tag="VF720_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7879818.1"
                     /translation="IHETAAAIESAGGRALAVRCDVREDDDLENVVAVTKSTLGRLDI
                     LIHNAGALWWTPVADTPIKRFDLVMQVNVRAAFVLTKLALPMLTAGGAGHVLVYSPPI
                     ELAALPGRTAYLISKFGMTMLALGLAGELREANVAANSLWPATAIETAATINFGVGGP
                     ATWRTPAIMADATLEIVATPPADLTGRQLLDEDVLRERGWTDFSRYRADPEHEPPRFA
                     LSELPRAAKVTQPG"
     gene            complement(684..>1762)
                     /locus_tag="VF720_10440"
     CDS             complement(684..>1762)
                     /locus_tag="VF720_10440"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7879819.1"
                     /translation="SLVMSDDIPWGKHWTTDIAVGPDGLLYVGVGSETNRGVNSNPWA
                     SSVLRFHPVNGFVDRYATGFRNPYGLAFHTDGSLFGTDNGAGPDSLWNCDEAEDELNW
                     IRPNGNYGFPACIASGSCGDVSASCTPPPCGAGDCQQFGGCDGSMTAPTLVLNPHSSS
                     DGLCFGKGFKGFTGNDLFIAQFGQQESLPGCFTDFGHKVVRTRLTKSGVVWTAGPPQD
                     FLTNIGRPLDVAVGPDSALYVADFAIGTIWRVFHTSTGVGVPDDDRLEGKAPPAMSFR
                     MTPNPAPGAVRLEWSHRPTGAVNVFVSDISGRLVRDLGSFAPGVTIAWDGQDATGRRA
                     APGLYLVTASSGDVTAAGRVILVE"
BASE COUNT          282 a          626 c          597 g          257 t
ORIGIN      
        1 catccacgaa acggccgcgg ccatcgagtc ggccggcggc cgggcgctgg ccgtccgctg
       61 cgacgtgcgc gaagacgacg acctcgagaa cgtggtggcc gtaacaaagt cgacgctcgg
      121 ccggctcgac atcctgatcc acaacgccgg cgcgctctgg tggaccccgg tggccgacac
      181 cccgatcaag cgcttcgacc tggtcatgca ggtcaacgtc cgcgcggcct tcgtgctcac
      241 gaagctggcg ctgccgatgc tcaccgcggg cggcgcggga catgtgctcg tctactcgcc
      301 gccgatcgag ctggccgcgc tgccggggcg caccgcctac ctcatcagca agttcggaat
      361 gacgatgctg gcgctcggcc tggccggcga gctccgcgaa gcaaacgtgg ccgccaactc
      421 cctctggccg gccaccgcca tcgaaaccgc cgccacgatc aactttggcg tcgggggccc
      481 ggccacctgg cgaacgccgg ccatcatggc cgacgccacg ctcgagatcg tggccacgcc
      541 accggccgat ctcacgggcc ggcagctcct cgacgaggat gtcctgcgcg agcgggggtg
      601 gaccgacttt tcccgctacc gggccgatcc cgagcacgag ccgccgcgct ttgcgttaag
      661 cgagctgccg cgcgcggcca aggtcactca accaggatga cccggccggc cgcggtcacg
      721 tcgccgctgc ttgccgtgac caggtagagg ccgggcgccg cgcggcggcc ggtggcatcc
      781 tgaccgtccc aggcgatcgt cacgcccggt gcaaacgaac cgaggtcgcg caccagtcgc
      841 ccgctgatat cggaaacgaa gacgttcacc gcccccgtcg gccgatgcga ccactcgagc
      901 cgcaccgcgc cgggcgctgg attgggcgtc atccggaagc tcatggccgg cggcgccttg
      961 ccttcgagcc gatcgtcatc cggcactccc acgcccgtgc tcgtgtgaaa caccctccag
     1021 atcgtgccga tggcaaagtc ggccacgtag agggcactgt cggggccgac ggccacgtcg
     1081 agcggccgcc cgatgttggt caggaagtcc tgcggcggcc ccgcggtcca gaccacgccg
     1141 gacttcgtca accgcgtgcg aaccaccttg tgtccgaaat cggtgaagca gcccggcagc
     1201 gactcctgct gtccgaactg ggcgatgaag aggtcgttgc cggtgaagcc cttgaaaccc
     1261 ttgccgaagc aaaggccgtc cgaggagctg tgcggattga gcaccagcgt gggcgctgtc
     1321 atcgagccgt cgcagccgcc gaactgctgg cagtcaccgg cgccgcaggg gggcggcgtg
     1381 caggacgccg acacgtcgcc gcagctcccg ctggcgatgc aggccgggaa gccgtagttg
     1441 ccgtttggcc ggatccagtt gagctcgtcc tcggcctcgt cgcagttcca gagcgagtct
     1501 gggccggcgc cattgtcggt gccgaaaagg ctgccatccg tgtggaaggc cagcccgtag
     1561 gggttgcgga agccggtggc gtaccggtcg acgaagccgt tgaccggatg aaaccggagc
     1621 acgctcgacg cccagggatt cgagttcacg ccgcggttcg tctcggagcc gacccctacg
     1681 tacaggaggc cgtccgggcc gaccgcgatg tcggtggtcc agtgcttccc ccaggggatg
     1741 tcgtcgctca tgaccagcga ct
//
LOCUS       DASVOD010000461         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_395097, whole genome shotgun sequence.
ACCESSION   DASVOD010000461 DASVOD010000000
VERSION     DASVOD010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395097"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VF720_10445"
     CDS             complement(<1..493)
                     /locus_tag="VF720_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7879820.1"
                     /translation="MATPTAARPAGSTDLGTINHWIDNKPYDGPVARWGDVTDPALGR
                     VTHRVAFADEALVNQAVAAAVKASATWRVSSLSARTRIFFAFRELLNARKRDVAELLT
                     REHGKVLSDAMGEVQRGLEVVDFACGLPQLLKGEFSENVSSEVDSYSIRQPLGVVAGI
                     TPFN"
     gene            complement(506..1852)
                     /locus_tag="VF720_10450"
     CDS             complement(506..1852)
                     /locus_tag="VF720_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7879821.1"
                     /translation="MNPETTAAPKTGLSGKEMAELCKKHSLYEWSAQSAIDPIPVAGA
                     KGVYFWTPEGKKYLDFNSQLMCTNIGHGHPKVIKAIQEQAATLAYANPFMATEPRARL
                     GQKLAEITPGDIDTFFFTNGGAEANENAIRIARVVSGRHKILVRYRSYHGGTAGALTL
                     TGDPRRWASEPGIPGVVRIPEFHKWGKAEPEPVAASLEAIEEVIRYEGPHTIAAIIME
                     TVVGTNGILIPPDGYMQGVREICDRHGILLIADEVMAGFGRTGKWFAVDHWNVVPDMI
                     SMAKGLTSAYVQLGAVGMRPHIAQAFRDKAFPGGLTYNSHPLACATALATLAVYEEEG
                     LIERAVRMGAELTKRLNDLKARHPSVGAVRNIGLFGIVELVRSRNPYTPMAPFNGTAP
                     EMAALGKHFRESGLYTLVRWNSFYTNPPLTITDAELDEGFAIIDKGLHAADAAVTG"
     gene            complement(1874..>2060)
                     /locus_tag="VF720_10455"
     CDS             complement(1874..>2060)
                     /locus_tag="VF720_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX7879822.1"
                     /translation="TISAKTHHQNVDYNLFEGMNVTGKPSVVMSRGEILVENDTLQVK
                     GGRGQFLKRPPGAPAVA"
BASE COUNT          308 a          665 c          701 g          386 t
ORIGIN      
        1 agttgaacgg cgtgataccg gccaccacgc cgaggggctg gcggatggaa tagctgtcga
       61 cctcgctcga cacgttctcg ctgaattcac ccttgagcag ctgcggcagg ccgcaggcga
      121 agtcgacgac ctcgaggccg cgctgcacct cccccatcgc gtcggagagc accttgccgt
      181 gctcgcgcgt gaggagttcg gccacgtcgc gcttccgcgc attcaacagc tcgcggaagg
      241 cgaagaagat gcgggtgcgc gcggagagtg atgacacgcg ccacgtggcc gatgccttga
      301 ccgccgcggc caccgcctgg ttcaccagcg cctcgtcggc gaaggccacg cggtgcgtga
      361 cgcggccgag ggcgggatcg gtgacgtcgc cccagcgggc caccggaccg tcgtacggtt
      421 tgttgtcgat ccaatggttg atcgtgccga gatcggtgct gccggccggc cgggcggcgg
      481 tgggcgttgc catggaatct ctctcctatc cggtcacggc cgcgtcggcg gcgtgaagtc
      541 ccttgtcgat gatggcgaac ccttcgtcga gctccgcgtc ggtgatcgtg agcggcgggt
      601 tggtgtagaa gctgttccag cgcaccagcg tgtagaggcc cgactcgcgg aagtgcttgc
      661 cgagcgcggc catctccggc gccgtgccgt tgaagggggc catcggcgtg tacggattgc
      721 ggctgcgcac cagctcgacg atgccgaaca acccaatgtt gcgaacggcg cctaccgagg
      781 gatggcgcgc cttcaggtcg ttcagacgct tcgtgagttc cgctcccatg cggaccgccc
      841 gctcgatgag cccttcctct tcgtacaccg ccagcgtggc cagcgcggtg gcgcaggcca
      901 gcggatggct gttgtaggtg aggccgccgg ggaaggcctt gtcgcggaag gcctgcgcga
      961 tgtgcggccg catgccgacc gcgccgagct gcacgtaggc cgaggtgagt cccttggcca
     1021 tgctgatcat atcgggcacc acattccagt gatcgacggc gaaccatttt ccggtgcggc
     1081 cgaagccggc cattacttca tccgcgatga ggagaatacc gtgccggtcg cagatctcac
     1141 gcacgccctg catgtagccg tccggcggga tcaggatgcc gttggtgccg acgaccgttt
     1201 ccatgatgat ggccgcgatc gtgtgcggcc cctcgtagcg gatgacctct tcgatggcct
     1261 cgaggctggc cgccaccggc tcgggctcgg cctttcccca cttgtggaat tcggggatgc
     1321 gcacgacgcc cggaatgccg ggctcgctgg cccagcgccg cggatctccc gtcagcgtga
     1381 gcgcaccggc cgtgccgccg tgatagctgc gatagcgcac caggatcttg tgccggccgc
     1441 tcaccacgcg ggcgatgcgg atggcattct cgttggcctc ggcgccgccg ttcgtgaaga
     1501 aaaaggtgtc gatgtcaccc ggcgtgattt cggccagctt ctggccaagg cgtgcgcgtg
     1561 gttccgtagc catgaacgga ttggcatacg cgagcgtggc cgcctgctcc tggatggcct
     1621 tgatcacctt cgggtggccg tgcccgatgt tggtgcacat gagctggctg ttgaagtcga
     1681 ggtacttctt cccctcgggc gtccagaagt acaccccctt cgcccccgcc accgggatcg
     1741 ggtcgatggc cgactgggct gaccactcgt acagggagtg cttcttgcag agctcggcca
     1801 tctccttgcc cgagaggccg gttttcggcg cggcggtcgt ttccggattc atcgaatgaa
     1861 agtcctcatg ggatcaggcc acggccggag cgcccggcgg gcgtttcagg aactggccgc
     1921 gaccgccctt cacctggagc gtgtcgttct cgacgaggat ctcgccgcgg ctcatcacga
     1981 cggacggctt gccggtgacg ttcatcccct cgaacaggtt gtagtccaca ttctgatgat
     2041 gcgtcttcgc ggagatcgtg
//
LOCUS       DASVOD010000462         5335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_813316, whole genome shotgun sequence.
ACCESSION   DASVOD010000462 DASVOD010000000
VERSION     DASVOD010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813316"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="VF720_10460"
     CDS             complement(<1..1689)
                     /locus_tag="VF720_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879823.1"
                     /translation="MGRVAAHRAAFLKAALLVFSVLLPLTFQAIPSARAQQNCGVAPG
                     SFAIDGQHYSAAGVVDWATGPAGQGVFDDNGNSLLVPSLYDRDEHWAGNSVDPDVFGG
                     HENKNNDDISAGGLPWQWGSGSGPQKNDITDVYAYSRFVGSDLWLYLGVTTRANNGAS
                     HLDFEFNQQGFSKTGTTSGLITGLGPNAGRTADVDFIVSVDFEGGGDVPVASFRKWQA
                     SGTGFEYLVETPDPGNVYICTNDTDIPAPPWGAVDPGGDDSDVLEAYQFVEIALNLTD
                     LDVDPSVFCSDVSTLLFKSRSAPSFTAELKDFALYQFSIIPTPDCTITADANPICAGT
                     TTTLCAPVAPPGFTYLYAWSGPGGPYPADRCITVSQGGNYSVTVTNTFGGCKGSTCQL
                     ELVVTPRPSCDITRDDAEICDGETTRFCGPTAPGGSTYDYAWSGPGGPFPNARCIDVS
                     REGTYNLVVTDRASGCSSGTCQASLVVHPLPPCDITGPSVICEDGTAQLCGPEGNYGY
                     AWTGPAGPYPATRCISVNVEGNYTLTVTDLTTGCARPACEHALDVVSKPVVEITG"
     gene            complement(1892..3799)
                     /locus_tag="VF720_10465"
     CDS             complement(1892..3799)
                     /locus_tag="VF720_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879824.1"
                     /translation="MRRVLFRLAALLLPLAVLALLEGGARLAGYGGNPPLIRKVADDG
                     DHAWYATNRPGTDIFFRGRTVAGGGMRELQFRTPKLPGTVRVVFVGESAIQGFPQPLP
                     LTNGSFLEALLHDAWGGQRRAEVLNLGATAVASYPAGRILEAALDHQPDLVVVMVGNN
                     AFYGACGVISLPPLLRSPFGVRLVHDLRSLGLAQWLEARLARPPATGGALMERMAAAG
                     RVGPESRLRGSAARILRDDLDAMVRTCARRGVPLILCTSPTNERDLHPVGETSGPAAE
                     AYARGTRLSVEGKTEEAAAAWIDARDLDPMPWRATTPLQETVRSAAQGGALFCDMEGA
                     FRKASDGGAIGWELLDDHVHMSLKGQWLFARTIAGVMTTMPGPLHVEPAALDSLPGWE
                     AYVAGLGHSEFSDYLAVSHLTTLFRIPFMRQANEAARQRFDERLRQIEESMSPGDLAA
                     AKRWRDPSLHGMSDRPVEFVAGVYRQAAGDFAGALELYRVARISVSVLSLWRLELDWR
                     IITCNRHLQDGPTDEDARLGREALKIGELLTENVARPNPDLLRYYGLAQNLAGDDAGA
                     IRSLEAVEPLPTGSDGWEVTVALADAYLGSGQREKAVALLTDATNRPETAEAARRALA
                     ELHAGPGMPSR"
     gene            complement(3796..4677)
                     /locus_tag="VF720_10470"
     CDS             complement(3796..4677)
                     /locus_tag="VF720_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879825.1"
                     /translation="MPRAASVVALLLLLGASGCSEDSREGSTSFRGYYPLEIGNRWEY
                     DRELTLEFYPDDPNSQPFEYGPVLAQATREIVGDAEQDGRDYRDVEYVIHEDGFPEVY
                     VSHEYHRQERAGHYVAYPPIDDDEASNRLAHLEWRAPMGPVAPTDAARRALVSLDEKR
                     AAFHRAMRAAVHDDDVPGTPPDGEGFELDYPLYRGSSWIVRPVDHPFLITREVVGMER
                     VEFPVGVRLSVVIRQDLPHLFGPNDHATFWYGRDGLYGYHLHLEAAWIDGGRPVGTYT
                     SDESERLSAIHLVVGND"
     gene            4842..>5335
                     /locus_tag="VF720_10475"
     CDS             4842..>5335
                     /locus_tag="VF720_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX7879826.1"
                     /translation="MRSPLSAALLALVLTVPATSRADDLVHTFSIVARDPETGDIGVA
                     VQSHWFSVGQSVPWAEPGVGAVATQSLVEVSYGPKGLALMREGMSAPDALARLLAEDP
                     QREVRQVAMIDAEGRVAVHTGNLCIAEAGHETGNQFSVQANLMLKESVWPAMAKAYRA
                     AKGDL"
BASE COUNT          854 a         1767 c         1860 g          854 t
ORIGIN      
        1 cccggtgatc tcgaccaccg gcttgctcac gacgtcgagt gcgtgctcgc acgccggacg
       61 ggcgcaaccg gtcgtgagat ccgtcaccgt gagcgtgtag ttcccttcca cgttcaccga
      121 gatgcagcgc gtggccgggt acgggccggc cggaccggtc caggcgtagc cgtaattccc
      181 ctcggggccg cacagctggg ccgttccgtc ttcgcagatg acgctcggcc cggtgatgtc
      241 gcacggcggc agtggatgaa cgaccagcga ggcctggcag gtgccactgc tgcagccgct
      301 ggcgcgatcg gtcacgacca ggttgtaagt accttcgcgg ctcacgtcga tgcagcgggc
      361 gttggggaac ggtccacccg ggccgctcca cgcgtagtcg taggtcgatc ctcccggcgc
      421 cgtcggtccg cagaagcggg tcgtctcgcc gtcacagatc tccgcatcgt cccgggtgat
      481 gtcgcaggac ggccgcggtg tcacgaccag ttcgagctgg caggtgcttc ccttgcagcc
      541 accgaaggtg ttggtgacgg tcacggagta gttgccgccc tggctcacgg tgatgcatcg
      601 gtcggcggga tacgggccgc ccgggccgct ccaggcgtaa aggtaggtga agcccggagg
      661 tgccaccggc gcacagaggg tggtggtggt gccggcgcag atcgggttgg catccgcggt
      721 gatcgtgcag tcgggggtcg ggatgatgct gaactggtag agcgcgaaat ccttcagctc
      781 ggccgtgaac gaaggcgccg agcggctctt gaagaggagt gtcgacacgt ccgaacagaa
      841 gaccgaggga tcgacgtcca ggtcggtgag gttcagcgcg atctccacga actggtacgc
      901 ctcgagcacg tcggagtcgt cgccacccgg atccacggct ccccaggggg gcgccgggat
      961 gtcggtgtcg ttggtgcaga tgtacacgtt gccgggatcg ggcgtctcga ccaggtattc
     1021 gaagccggta cccgaggcct gccacttgcg gaagctcgcc acggggacat cgccgccccc
     1081 ttcgaagtcg acgctgacga tgaagtcgac gtcggcggtg cgcccggcgt tcggcccgag
     1141 gccggtgatc aggcccgagg tcgtgccggt cttgctgaat ccctgctggt tgaactcgaa
     1201 gtcgaggtgg ctggcgccgt tgttggcgcg cgtggtcacg cccagataga gccagaggtc
     1261 cgagccgacg aaccgcgaat aggcatacac gtcggtgatg tcgttcttct gcgggccgct
     1321 gccggaaccc cactgccagg gaagcccgcc ggccgagatg tcgtcgttgt tcttgttctc
     1381 atggccgccg aagacgtcgg ggtcgacgct gttgccggcc cagtgctcgt cgcggtcgta
     1441 cagcgaggga acgagcagcg agttgccgtt gtcgtcgaag acgccctgac cggcgggacc
     1501 ggtggcccag tccaccacgc cggccgcgga gtaatgctgg ccgtcgatgg cgaacgaccc
     1561 gggcgccacg ccgcaattct gctgggcgcg cgcggacggg atcgcctgaa aggtgagcgg
     1621 gaggagaacg gagaaaacga ggagtgccgc cttgagaaag gcagcccggt gcgcggccac
     1681 gcggcccatg tagtaccccc tggtgcgagt cgaaaggtgt cgtgagcgaa ggggtgatgt
     1741 cgtcaggaaa agagtagcac tatccgtggg ccatcccgct cgaatcctgc aggaggcaac
     1801 ggaagcttcg atggaaatgc gcgatttgac ccgttccgtg cttcaataag taagctattt
     1861 cgacagtttc cggggcgacc tggcggcttc gtcagcggga aggcattccg ggcccggcgt
     1921 ggagctcggc cagcgcgcgc ctcgccgcct cggccgtctc cggccggttc gtggcgtccg
     1981 tgagcaatgc cacggccttt tcccgctggc cgctgccgag gtaggcatcg gccagggcga
     2041 ccgtcacttc ccacccgtcg gagccggtcg gcagcggctc aaccgcctcg agggaccgga
     2101 tggcgcccgc atcatccccc gcgaggttct gcgcgaggcc gtagtaccgg agcaggtcgg
     2161 gattcggtcg ggccacgttt tccgtgagca gttcgccgat cttcaatgcc tcgcggccca
     2221 ggcgggcatc ttcgtcggtc gggccatcct gcagatgccg attgcaggtg atgattcgcc
     2281 agtccagttc gagccgccag agcgagagca ccgacactga gatccgcgcc acgcgataca
     2341 gctcgagcgc accggcgaaa tcgccggcgg cctggcgata cactcccgcc acgaactcca
     2401 cgggacgatc ggacatcccg tgcagggatg gatcgcgcca gcgcttggcg gccgccaggt
     2461 caccgggcga catcgattcc tcgatctgcc gcagccgttc gtcgaatcgt tgccgtgccg
     2521 cctcgttggc ctgcctcatg aacgggatgc ggaacagcgt ggtgaggtgc gagacggcca
     2581 ggtagtcgct gaactcgctg tggccgagcc ccgccacgta ggcctcccag ccgggcaacg
     2641 aatcgagcgc cgccggttcc acgtgaaggg ggccgggcat cgtcgtcatc acgccggcta
     2701 tcgtgcgcgc gaaaagccac tgtcctttga gcgacatgtg cacatggtcg tcgagcagct
     2761 cccagccgat cgcaccgccg tcgctggcct tccggaaggc accttccatg tcgcagaaga
     2821 gcgcgcctcc ctgcgcggcc gatcgcaccg tttcctggag cggggtggtg gctctccagg
     2881 gcatcgggtc aaggtcacgc gcgtcgatcc atgccgcggc ggcctcctcg gtcttcccct
     2941 cgaccgacag ccgcgtgccg cgggcgtacg cctcggccgc cgggccgctc gtctcgccga
     3001 ccgggtggag atcgcgctcg ttggtgggcg acgtgcagag gatgagcggc acgccccgtc
     3061 gcgcgcaggt ccgcaccatc gcgtccaggt cgtcccgaag aatccgggcg gcggagccgc
     3121 gcaacctgct ctccggcccg acacgaccgg ccgccgccat cctctccatc aacgcgccgc
     3181 cggtggccgg aggacgggcc aggcgggctt ccagccattg cgcgaggccc aggctgcgga
     3241 ggtcatgcac gagacggact ccgaacggcg accgcagaag cggcggcaat gaaatgacgc
     3301 cgcaggcccc gtagaacgcg ttgttcccga ccatgaccac gacgaggtcg ggctggtgat
     3361 cgagcgccgc ctcgaggatc cggccggccg ggtagctggc caccgcggtg gcgcccagat
     3421 tcagcacctc cgcccgccgc tgcccgcccc acgcatcgtg caggagggcc tcgaggaacg
     3481 acccgttggt caacggcagc ggctgcggga atccctggat cgccgactcc cccacgaaga
     3541 cgacgcgcac cgtgccggga agcttcggcg tgcggaactg caactcgcgc atcccgccgc
     3601 cagccaccgt tcggccacgg aagaagatgt ccgtgcccgg tcgattcgtg gcataccagg
     3661 cgtgatcgcc gtcatcggcc actttccgga tcagcggtgg attgccgccg tagccggcca
     3721 gccgggcgcc cccttccagc agggccagga cggccagcgg caggaggagc gcggccaggc
     3781 ggaagaggac tcgcctcaat cgttacccac cacgagatgg attgccgaga ggcgttccga
     3841 ctcatcggag gtgtaggtgc cgacgggtcg accgccatcg atccaggcgg cttcgaggtg
     3901 caggtggtat ccgtagagac cgtcgcgccc ataccagaac gtcgcgtgat cgttgggtcc
     3961 gaagagatgg ggaaggtcct gccggatcac gacggaaagc cgcacgccga ccgggaattc
     4021 cacgcgctcc atcccgacga cctcgcgcgt gatcaggaag gggtgatcca ccggccgcac
     4081 gatccacgag cttccccgat agagcggata gtccagctcg aatccctcgc cgtccggtgg
     4141 cgtgcccggg acgtcgtcgt cgtggacggc ggcgcgcatc gcgcgatgaa aggccgcgcg
     4201 tttttcatca agcgaaacga gcgcccgtcg agccgcatcg gtaggcgcca ccgggcccat
     4261 cggcgcccgc cactcgaggt gcgcgagcct gttcgaagcc tcgtcatcgt cgatgggggg
     4321 gtaggccacg tagtgcccgg cgcgttcctg ccggtggtat tcgtgcgata cgtacacctc
     4381 gggaaagccg tcttcgtgaa tgacatattc cacgtcgcgg tagtcccggc cgtcctgctc
     4441 cgcgtctccc acgatttccc gcgtggcctg ggccaggacg gggccatatt cgaatggttg
     4501 cgagttcggg tcgtcgggat agaactccag cgtcagctcc cggtcgtatt cccagcggtt
     4561 gccgatctcc agtgggtagt agccgcggaa cgaggtgctt ccctcgcggg agtcctccga
     4621 gcagccggag gctccaagca gcagcaggag ggcaacgaca ctggcggcgc gtggcatggg
     4681 atccttcctg ggaggagcgg gcatcgcggg atgcagagca ccatgatcgg gcgatccgga
     4741 cccggcggca agtgctttgc gagccgcgtt gacgcggcgg gtccccggcg cttcactcta
     4801 cccccggcca cccgtttcgt atcgccagga ggatgccccc catgaggtcc ccgctttccg
     4861 ccgcgttgct ggccctggtc ctgaccgttc ccgccacttc ccgcgccgat gacctcgtcc
     4921 acaccttctc catcgtggcc cgagaccccg aaaccggcga tatcggcgtg gccgtgcagt
     4981 cgcactggtt ctcggtgggg cagtcggtgc cgtgggccga gccgggcgtg ggggccgtgg
     5041 ccacgcaatc gctggtggag gtgtcatacg gcccgaaggg gctcgcgttg atgcgcgagg
     5101 ggatgtccgc ccccgatgcg ctggccaggc tcctggccga ggacccgcag cgcgaggtgc
     5161 gccaggtggc gatgatcgac gcagaggggc gcgtggccgt acacaccggc aacctctgca
     5221 ttgccgaagc cgggcacgag accggcaacc agttctccgt gcaggccaac ctcatgctga
     5281 aggagagcgt gtggcccgcg atggccaagg cgtatcgcgc cgcgaagggc gatct
//
LOCUS       DASVOD010000463         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_448780, whole genome shotgun sequence.
ACCESSION   DASVOD010000463 DASVOD010000000
VERSION     DASVOD010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448780"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1668
                     /locus_tag="VF720_10480"
     CDS             <1..1668
                     /locus_tag="VF720_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879827.1"
                     /translation="ALSPFIAWPAAGCVVTSVMADRSWAGATLDRLAMLDAAAVPPSN
                     FVKPSAMKPPAMPRFGGFIDAPGQAFSRGEAGARAMSPHIDRRLHGVCFAHEHGLEWG
                     YVSREAADNLTYLRDEVNVSAISISPFGYLAGATDTRIRRFGRGRDSHDEDEETDESL
                     IAVTARAHERGMDVLLAPHLWGRVWCGDWHAEDEEGWAALFASYREFALHYAMLAAYS
                     GADVYQVGKELKATTGREREWRELIAQVRLIYPGAITYGANWDEYPRIAWWDAVDLIG
                     INQYTPVAHGDRISDSGARIAMSVMADSIDALSQRVDRLWFLTEIGFTAAANGYREPW
                     KSFDPAVDTGDPVRQAMLYEAALETYLPRSRCRGLFWWKWFTRLDSNRFGRNRFDFPP
                     YGKMAEQVLAARFGKRPEPGRDRERVYNDVVQAIDFRLYRGLARTNEQSETHADWSRS
                     DLLRVEGEMFYPQPVAITAADVESLRTWSARQLAGTEPIMCHFHADWLLEWRAGDELT
                     RQLVCLGCGEVRTSSHWSSPRVRLTDPERAALDGILAKYRGPGPRQP"
     gene            1796..>2449
                     /locus_tag="VF720_10485"
     CDS             1796..>2449
                     /locus_tag="VF720_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879828.1"
                     /translation="MKSLFQKIVLSACLVPTVLAAPALALEPSRDIESYVPFALTDLD
                     FKGRNADPTRGFITGGNVGVNCIDDFDPTAPLMGFGANGAVVMSNGMQVVTDSGRINS
                     NSDLYDLFVNDLWGSSMPNIRHSGPNRQLRRARLHGHVRVLLDRCRLERRHGVVRQEH
                     HHLRPFLGAVWPDQPRPLHRPVRKVLGRPDRQRLERQHGTVRKSAGDHRTVDLERDQD
                     "
BASE COUNT          411 a          824 c          788 g          426 t
ORIGIN      
        1 gcgctgtcac cgttcatcgc ctggcccgcc gccggctgtg tcgtcacgtc agtaatggcg
       61 gatcgctcat gggcgggcgc gacgctcgac cggcttgcga tgctcgacgc tgcggccgtc
      121 ccgccctcca atttcgtcaa acccagtgcc atgaagccgc ccgccatgcc ccgcttcggt
      181 ggcttcatcg atgctcccgg ccaggcattt tcacgtggcg aagcgggcgc ccgcgccatg
      241 agcccgcaca tcgaccgacg gctgcacggc gtctgcttcg cgcacgagca tggcctcgag
      301 tggggttacg tgtcgcggga ggcggcggac aatctcacct acctgcgtga cgaggtgaat
      361 gtctcggcca tttcgatcag tccctttgga tacctggccg gggccaccga tacccggata
      421 aggcgattcg gtcgaggtcg cgacagccac gacgaggacg aggaaaccga cgagagcctc
      481 attgccgtga ccgcgcgggc ccacgaacga ggaatggacg tcctactcgc gccgcatctc
      541 tgggggcgcg tctggtgcgg cgactggcat gccgaggatg aggagggatg ggcggcgctc
      601 ttcgcatcct atcgcgagtt cgccctgcac tacgcgatgc tggcggcgta ctccggagcc
      661 gacgtctacc aggtcgggaa ggaattgaaa gccacgaccg gtcgcgagcg ggagtggcgc
      721 gagctcatcg cccaggtgcg gctcatctat cccggagcca tcacctacgg cgccaactgg
      781 gatgagtacc cccgtatcgc gtggtgggat gcggtggacc tgatcggcat caaccagtac
      841 acgccggtgg cccacggcga tcggatcagc gattcgggcg cgcgcatcgc catgtcggtg
      901 atggccgatt cgatcgatgc cctgtcgcag cgcgtcgacc gactgtggtt cctgaccgag
      961 atcggattca ccgccgcggc caacggctac cgggaaccgt ggaagtcgtt cgatccggcg
     1021 gtcgataccg gcgacccggt ccggcaggcg atgctctacg aggccgccct cgaaacctat
     1081 cttcctcgat cgcgctgccg cggcctcttc tggtggaagt ggttcacccg cctggattcc
     1141 aacagattcg gccgcaaccg cttcgacttc ccgccctatg gaaagatggc cgagcaggtg
     1201 ctggcggccc gcttcggcaa acggcccgag ccgggccgcg accgggagcg ggtgtacaac
     1261 gatgtcgtgc aggcgatcga tttccgactg tatcgcggcc ttgctcgcac gaacgagcag
     1321 tccgaaacgc acgccgactg gagccggagc gacctccttc gtgtcgaggg ggagatgttc
     1381 tatccgcagc cggtggcgat aaccgccgcc gacgtggaat cgctccggac atggtcggcc
     1441 cgacagctgg ccggaaccga gccgatcatg tgccacttcc acgccgactg gttactcgag
     1501 tggcgtgccg gtgacgagct cacccgacag ctcgtgtgcc tgggctgcgg ggaggtgcgc
     1561 acctcgagcc attggtcctc accgcgggtg cgattgaccg acccggaacg ggcggcgctg
     1621 gacggcatcc tggcgaagta ccggggcccc ggcccccggc agccctgaga gatcccggcc
     1681 gtcggatacc actctcgtcg tatcaggcgt cggggcactt cgcggggctt cgacggctga
     1741 tccggcgcac cctccgtgct attcatgtcc attccacgac accctcggag gccgcatgaa
     1801 atccttgttt cagaagatcg tcctatccgc atgccttgtt cccaccgtcc ttgccgctcc
     1861 cgccctcgcc ctcgaaccgt cgcgcgacat cgagtcgtac gtccccttcg cgctgaccga
     1921 cctcgatttc aaggggcgca atgccgatcc gacgcgtggg ttcatcaccg gcggcaatgt
     1981 cggcgtgaac tgcatcgacg acttcgaccc gacggccccg ttgatgggct tcggcgccaa
     2041 tggcgcggtc gtgatgtcca acggcatgca ggtggtgacg gacagcgggc gcatcaacag
     2101 caactcggac ctgtacgacc tgttcgtcaa cgacctgtgg ggatcgtcga tgccgaacat
     2161 ccgtcactcg ggcccgaaca ggcagctacg tcgggcccgc ctgcacggcc acgttcgagt
     2221 tctgctcgac cggtgtcggc tcgaacgacg ccacggtgtc gttcggcagg aacaccatca
     2281 tctacggccg tttctgggcg ccgtatggcc ggatcaacct cggccactcc accgacctgt
     2341 acggaaagtt ctgggccgac cggatcggca gcgactggaa cgtcaacatg gaactgtgcg
     2401 gaaatccgcc ggtgaccatc gcaccgtcga cctggagcgc gatcaagac
//
LOCUS       DASVOD010000464         2860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_448811, whole genome shotgun sequence.
ACCESSION   DASVOD010000464 DASVOD010000000
VERSION     DASVOD010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448811"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..2551)
                     /locus_tag="VF720_10490"
     CDS             complement(53..2551)
                     /locus_tag="VF720_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879829.1"
                     /translation="MRTAARCHVRRFLTLLLTLATLSFATAARASHETIVIDGDLTDL
                     ITAVNNNIGADKGGFAAPDALGDTYTMVCGYTNGFDIINAYVLLDFKDAMGNATPNDV
                     TLYVGWDMDGIVGDVDGNGNPNTFDLGSPGGSTGCALADEPGIGPNESYNLLLDLDCA
                     GAPDDIRIQVKDNTVTRVVNGMPSVIPGATFMASGDKLELKVPNYQNLLTGTMITADL
                     CDARMRLTANAAFDGPGEDFSSPLTLLIPPSIDVTKNPPTRTVCAGSPVSWAITVTNN
                     GLCRIHALNVEDVLGAGMTFDNSDPIATSVMGQTIRWEFANLELDPGESMQIDLTATT
                     AAPCASGTLTNNVSVEAVHLTPCLPAGTPPPTANDSASATVTCRDVPPCDITGDPHTC
                     FPSSETYTANVGAGYNFSWSFNSVPPGICSTSDPLDGSSVTVDFTGAGVCTVSLTVTD
                     PENPEGCNTTCNYIVTSEAQPPCNIDGVDEICALNNSVFTTNVGGTYSKAWSVSSVPA
                     GIASISGASDGDQVTVNFSGPGTATLQLRVGNPADPVDCVSMCEHPVTVNQCVEEPDL
                     CWMTGGGCLDEPVKKSHKNHSFGGNIGPPPDGSWEHIVREKNKIQFNFHSWDAHVDSC
                     WVDGLDGPCSPHGEINNIAWSGTGKFSVGNGVREEDATFKAHVADHGEPGSHDGHCGS
                     EDFYEITVYHLDGSTAFHAEGYLSCGNLQLHPFHGNQGPGPVDWRALINEPADVIDLL
                     VTRPFPNPVRSGAVSIEYRIPTGTQANVRVTLFDVSGRVVRTLEPGQQASGGHSVDWD
                     LRDEDGSEVPNGIYFYRLTVGTESVTEKLMVVRQ"
BASE COUNT          464 a          857 c         1017 g          522 t
ORIGIN      
        1 aagggccgct ccccgctcca gttacgtgcc aggactggcg aggtgtcgtg gcttactgcc
       61 ggaccaccat gagcttctcg gtgaccgact ccgttccgac cgtcagacgg tagaagtaga
      121 tcccgttcgg cacctcgctg ccgtcctcgt cccgcaggtc ccagtcgacg gagtgaccac
      181 ccgaagcctg ctgtcccggc tccagtgtcc ggacgacccg gccagacacg tcgaagagcg
      241 tgacgcggac gttcgcctgg gtgccggtcg ggatgcggta ctcgatcgac accgcacccg
      301 aacggaccgg gttcgggaag ggacgcgtga ccaggagatc gatcacatcg gccggttcgt
      361 tgatgagcgc ccgccagtca accgggccgg ggccctggtt gccgtggaac gggtgaagct
      421 ggaggttgcc gcacgacagg taaccctcgg cgtggaacgc cgtcgagccg tcgaggtggt
      481 agaccgtgat ctcgtaaaaa tcttcgctgc cgcagtggcc gtcatggctc cccggctccc
      541 cgtgatccgc cacatgcgcc ttgaacgtgg cgtcttcctc gcgcacgccg ttcccgacgc
      601 tgaacttgcc ggttccggac caggcgatgt tgttgatctc gccgtgcggg ctgcacgggc
      661 cgtcgaggcc gtcgacccag cacgagtcga catgggcgtc ccagctgtgg aagttgaact
      721 ggatcttgtt cttctcacgg acgatatgct cccacgagcc gtccgggggc ggcccgatgt
      781 tgcctccgaa cgagtggttc ttgtggctct tcttgacggg ctcgtcgagg cagccgccgc
      841 cggtcatcca gcagaggtcg ggctcttcca cgcactggtt gaccgtgacg gggtgctcgc
      901 acatcgatac gcagtcgacc gggtcggccg ggttaccgac gcgcagctgg agggtggccg
      961 tgccggggcc ggagaaattg accgtaacct ggtcgccatc gctggcgccg ctgatcgagg
     1021 cgataccggc cggcaccgag ctgaccgacc aggccttcga gtaagtgccg ccgacattcg
     1081 tcgtgaagac cgagttgttc agcgcgcaga tttcatccac gccatcgatg ttgcagggcg
     1141 gctgggcctc ggacgtcacg atgtaattgc acgtggtgtt gcacccttcc gggttctcgg
     1201 gatcggtgac cgtgaggctg accgtgcaga cgccggcacc cgtgaagtcg accgtgaccg
     1261 agctgccgtc cagcggatcg gaggtgctgc agatccccgg ggggaccgag ttgaacgacc
     1321 acgagaagtt gtagcccgcg ccgacgttgg ccgtgtaggt ctccgacgac gggaagcagg
     1381 tgtgcgggtc gccggtgatg tcacacggcg gcacgtcgcg gcaggtgacc gtggccgaag
     1441 ccgagtcgtt ggccgtcggc ggcggggtgc cggccggcag gcagggggtg aggtgcaccg
     1501 cctcgaccga cacgttgttg gtgagggtgc cggaggcgca cggggccgcg gtcgtggcgg
     1561 tcaggtcgat ctgcatcgat tcgccgggat cgagctcgag attcgcgaat tcccagcgga
     1621 ttgtctgccc catgaccgac gtggcgatcg ggtcgctgtt gtcgaaggtc atgccggctc
     1681 ccaggacgtc ttcgacgttc agggcgtgga tgcggcagag gccgttgttg gtgactgtga
     1741 tcgcccagga aaccggcgag ccggcacaga cggtgcgagt cggcggattc ttcgttacgt
     1801 caatcgacgg cgggatgagc agggtgaggg ggctggaaaa gtcctcgccc gggccgtcga
     1861 aggcggcatt ggcggtcagg cgcatgcggg catcgcacag gtcggcggtg atcatcgtgc
     1921 ccgtcaggag gttctggtaa ttcgggacct tgagctcgag cttgtcgccc gaggccatga
     1981 aggtggcgcc cgggatgacc gacggcatgc cgttcacgac acgggtgacg gtgttgtcct
     2041 tcacctggat ccggatgtcg tccggagcac cggcgcagtc gaggtcgagc agcaggttgt
     2101 acgactcgtt ggggccgatg cccggctcgt cggcgagggc gcagccggtc gagccgccgg
     2161 ggctgccgag gtcgaaggtg tttggattgc cgttgccgtc gacgtccccg acgatcccgt
     2221 ccatgtccca acccacgtag agggtgacgt cgttcggcgt ggcattcccc atggcgtctt
     2281 tgaagtcgag caggacgtag gcgttgatga tgtcaaagcc gttcgtgtag ccgcacacca
     2341 tcgtgtaggt gtcgccgagc gcgtccggtg cggcaaagcc acccttgtcc gctccaatgt
     2401 tgttgttgac cgccgtgatc aggtcggtca ggtcgccgtc gatcacgatc gtttcgtgcg
     2461 acgcgcgggc cgccgttgcg aatgacagcg tggccaacgt caggagcaat gtgaggaaac
     2521 ggcggacatg acatcgtgct gcggtgcgca tgtgagaacc cccctgttgc cggccatggc
     2581 ccggctgccg agggatctcc acgtgcgatg ggagggagcg gccggtgctg gctggcggtg
     2641 tcgagacatg gccgggtgcc gccctgcagg ccggcggcaa cggctgtcgg cggagtttcc
     2701 aattaatgtg tgagtgtgtc aatactaaag agatatcagg gaaattgcgt gcaggccgta
     2761 tggaaagcgc cttgcaggac ttccgggatg cacctcgccc agcgaacgcg gaccaataga
     2821 aaacgggggg tgatccgaag accacccccc gtcgattgct
//
LOCUS       DASVOD010000465         7530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_239449, whole genome shotgun sequence.
ACCESSION   DASVOD010000465 DASVOD010000000
VERSION     DASVOD010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239449"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VF720_10495"
     CDS             complement(<1..876)
                     /locus_tag="VF720_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879830.1"
                     /translation="MIALTATRTEAGLFPAASTGDLWFQADHAGFRDSTGAIIEEFYI
                     RLPNDQIVFNESESDSGVDFEGRVFAKLEFHDESGDRIGEASRPFEFHAGSLEQASSP
                     DRVQLLVIREPFHPRTHDVTVTVEDLNAKKRGLIYLFTKKKKNGALRARISAPSDSGA
                     IGVSDLQFAWSVRRAPPGSPFYKNGWSVVPNPGRNFGLYLDTLHVYSEIRDRSAATDS
                     AWLAVKTVRDATGRVLGSESDTLSLDDGFGIQLVTMPLAGLPAGTYDLSIHLTGLSSG
                     RTAESIRAFNVLWDEG"
     gene            complement(983..1924)
                     /locus_tag="VF720_10500"
     CDS             complement(983..1924)
                     /locus_tag="VF720_10500"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX7879831.1"
                     /translation="MKRIVAALALLASTANLALAAEPFDKVGTVGAQFLKINIGARAT
                     GMGGSFTAVADDASSVYWNPAGISRLSGNVFTVNHAVWLTDISVTQAAYIFDVGFMPG
                     MFAVQAKSLYMSDMERTTVFHPDGDGTFFDAGDSQFGLSYSRSLTDKFSAGLTASLVH
                     SGLDDLSDNVISFDFGTLYDTGYKSLRIGFAIQNIGGDLTYIDDARAAKVPTIFRVGA
                     SMDVVQSGNNNLLMSAEFSHPPDNSERVNVGGEYGFKDFLFARAGYNIGYDSEKLAAG
                     LGVKFPTSQSTETRFDYSYTDMSDLGGAHRFSLDLAF"
     gene            complement(1988..4423)
                     /locus_tag="VF720_10505"
     CDS             complement(1988..4423)
                     /locus_tag="VF720_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879832.1"
                     /translation="MTMNRCGRRPWLIAAAMGITAGGLCLPPGAEARIDLNDPANGAR
                     NIRAVYANRDPYIFTHNVGLLTLQITNIGIIGNPFIDELSAGWRGGEYLYLGGLWLGA
                     IGEDQEPHVSTAVPVELRPSLLPVDTIYESYEGIRGGNRNTEALDADDDDDGLEDEDF
                     HNGKDDDLDGQVDEDYAAIGQQMYSCEYRDNTTEATNQLNEHRPLGLLVHQRSFQWAT
                     NDINEFVGFEFEVTNIGDQRLQDMFIGFNVDADAGPKSMNNYWTDDLVGFVRVDTLDR
                     DPNQSGQCANQQVSMDIAYIWDAPDNGTTIVGGDVPGYFGGMFLGHTTDPAGVKAPVR
                     VGLSTVKWTSSTGAYPQGDPRTDAERYDLLSRREIPTRNANKPDDYRFTLSAGPFLTL
                     DPGDKLVFQTAFVIGEGLQGMITNAVNAQRVFNGQYLDADGNPETGVEGKERCLIAAD
                     PRFPVMWDDPCDTLGTTVNISFRTCIPAAYVDDDCNSCTGIGGKEALLNWVGTTAPPP
                     PATNTDPELFGDPEAPAAPNSEIYLSAPQGDRRVILQWDNVSELSRDPITGNDIFEGY
                     KIWRVDNWQRPEGSIGPTPQEWMQVAEFRKNPRDKLGSNSPRSLRRVLHPERDEIKQT
                     TQFGPQYYIGRYEWQDSSGITNGKVYFYAITAFGVQTRRNPVTGLLETIELGGSPTAV
                     QKEVVVASWKNGTSCDQVKVVPNPYRGGADWDLEPSDCDPTGTKIAIRNLPEGWTRLR
                     IYSLTGDLIIEADPSDSRVVGTCDNSAGNLQTGTFYWDLITRSGQNVVAGVYLYVVEA
                     PVGSCRGRFVVIR"
     gene            complement(4506..7205)
                     /locus_tag="VF720_10510"
     CDS             complement(4506..7205)
                     /locus_tag="VF720_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7879833.1"
                     /translation="MSRGIALFRSGLAILIVLLAGLSLANLAHAAAAGTITGIVVGPS
                     GQPESQAIVQVEGSTPPLGAPTDAAGNFTIRNVPVGTYRLLVKKFGFQDLIRPNVVIS
                     ANQTTTIKFELKETVATEIDEVNVTGARTRMERESSTTKQKIGKEDLESLPVDTYQEA
                     VALKAGVVSQAGELHFRGGRSGEVAYMIDGIRVSDPLAGGGLDIATNAVAESEILLGG
                     FDAEYGNAQSGVINISTQEGGASFSGAVEYSTDDFGAPDKTYNNFDRLNVGFGGPTGV
                     KDLTYFFSFQGTFSDGYLKTNEVRPRQTILDFIRVGPRQNNDFNYQGKLAWRPAPSTK
                     LTLEYLNNHSVRDIYTHIFSLNGFVQTRVDTIRATGQIVTRYGRFSAESEDSTFVPYN
                     ASEHTPNVEDNFNQVKLVWNHTLSPATFYTVKVSRYAFDYDYRVKEQEPWEYNGRYPD
                     QWRNAIDFTTSRFFATNGDYPQFADRNTQTFSFKTDWTHQVGRHKFKSGIEGVYNDLS
                     LLSIDFPVSVNANGQVGGFRSQYHYFNNEGAFYLQDRWDHEGMVINVGGRVDVFSVGD
                     QLGVSEVQDRSRNQFSPRVGIAYPVSDRDVFSFHYGRFSQIPDRQYIFENRGAATQVR
                     GNPNLENETTVSYQAGLQHMFTQDIFGQFSVYFKDIFGLLTIEQLSSGDNPNLVPTYV
                     NRDYASARGFELSLEKRFSHNFSGGVSYGYGVATGVASDPNLQRNEELLYLPISEQPL
                     DWDQRHTFSAQVLLSQPAEWAVNMVWSYGSGFPYTPTSRNERKVDPSLANAARLPSTT
                     KLDIQAEKHFSIYNQDVKVFFRGNNVLDARNLNDLEPSNWPNPPGTNTNDYRVFYTET
                     GRAGGAYLGDDVNGDATEDWIPVNDPRVFTEGRNLRLGVGVKF"
BASE COUNT         1269 a         2242 c         2622 g         1397 t
ORIGIN      
        1 tccctcgtcc cacaggacgt tgaacgcgcg gatcgactcg gcggtgcgcc cgctcgagag
       61 tcctgtcaga tggatcgaga ggtcgtacgt gccggccggg aggccggcca acggcatcgt
      121 gaccagctgg atgccgaagc cgtcatccag ggagagcgtg tcgctctcgc tcccgagaac
      181 cctcccggtg gcatcccgga cggtcttgac ggccagccag gcgctgtcgg ttgccgcgga
      241 ccggtcacgt atttcggagt agacgtgcag cgtgtccagg tagagaccga agttgcgacc
      301 ggggttcggg accaccgacc agccgttctt gtagaaggga ctgccgggag gcgcccgccg
      361 gaccgaccag gcgaactgca ggtcggaaac gccgatcgcg ccggagtcgg acggggccga
      421 gatccgggcc cgcaacgcac cgttcttctt cttcttggtg aagaggtaga tgagtccccg
      481 cttcttggcg ttcaaatcct ccacggtcac ggtcacgtcg tgcgtgcggg gatggaacgg
      541 ctcccggatg acgaggagct gaacgcggtc gggcgagctg gcctgctcga gcgagccggc
      601 gtggaattcg aacggacggg aggcctcgcc gatccggtcg cccgattcgt cgtggaactc
      661 gagcttcgcg aagacgcgcc cctcgaagtc aacgcccgag tcgctctccg actcgttgaa
      721 aacgatctgg tcgttgggga gccggatgta gaactcctcg atgatcgccc cggtggaatc
      781 gcggaagccg gcatgatcgg cctggaacca gaggtcgccg gtggaggccg ctggaaagag
      841 cccggcctct gtccgcgtgg ccgtgagggc gatcatgagg gcaaaggttc ccgccaacca
      901 gccggaaagc gacaggccgc cccccgacgg ggagcggcct gtcgatactt ccgtcagacg
      961 gtgccggccc agccggcctt cctcagaacg cgaggtcgag cgagaagcga tgggcgcctc
     1021 ccaggtccga catgtcggtg tacgagtagt cgaagcgcgt ttcggtggac tggctggtcg
     1081 ggaacttcac gccgagcccg gccgcgagct tctcgctgtc gtatccgatg ttgtagccgg
     1141 cgcgggcgaa gaggaagtcc ttgaagccgt actcgccacc cacgttcacc cgctcgctgt
     1201 tgtcgggcgg atgggagaac tcggccgaca tgagcaggtt gttgttgccg gactggacca
     1261 cgtccatcga ggcgccgaca cggaagatcg tggggacctt ggccgcgcga gcgtcgtcga
     1321 tgtaggtgag gtcgccgccg atgttctgga tggcgaaccc gatccggagc gacttgtaac
     1381 ccgtgtcgta cagtgtgccg aaatcgaacg agatcacgtt gtccgacagg tcatcgagcc
     1441 ccgagtgaac caggctggcg gtgaggcccg cggagaactt gtcggtcagc gagcgcgagt
     1501 acgacaggcc gaactggctg tcgccggcgt cgaagaaggt gccgtctcca tccgggtgga
     1561 acacggtggt gcgctccata tcgctcatgt agagcgactt ggcctgcacc gcgaacatcc
     1621 ccggcatgaa gccgacgtca aagatgtagg ctgcctgcgt caccgagatg tcggtgagcc
     1681 acaccgcatg gttgaccgtg aagacgtttc ccgacaggcg tgaaatgccg gccgggttcc
     1741 agtagacgga cgaagcgtcg tcggcaacgg cggtgaagct gccccccatg ccggtggcgc
     1801 gggcgccgat gttgatcttg aggaactggg ctccaacggt tccgaccttg tcgaagggtt
     1861 cggcggccag ggccaggttg gcagtggatg ccagcaacgc cagagcggcg acgattcgtt
     1921 tcactggata cctcccggaa cgggtcggtc gtcccgactt gtcacgacgg tccaagggga
     1981 ccccctccta tcggatgacg acgaagcgac cgcggcagga acccaccggt gcctcgacga
     2041 cgtaaaggta aacgccggcc accacgttct ggccgctgcg ggtaatcaga tcccagtaga
     2101 acgtcccggt ctggaggttg cccgcgctgt tgtcgcaggt acccaccaca cgcgagtcgc
     2161 tcgggtccgc ctcgatgatc aggtctccgg taagggagta gatcctgagc cgggtccacc
     2221 cttccgggag gttccggatg gcgatcttgg tgccggtcgg atcgcagtcc gaaggctcca
     2281 gatcccagtc ggcaccgccg cggtagggat tcggcacgac cttgacctgg tcgcaactcg
     2341 tcccgttctt ccaggaggcc accacgacct ccttctggac cgccgtcggg ctgccgccca
     2401 gctcgatcgt ctcgagaagg ccggtgaccg ggttccggcg cgtctgcaca ccgaacgcgg
     2461 tgatggcgta gaagtagacc ttgccgttgg tgatgcccga ggaatcctgc cattcgtacc
     2521 gcccgatgta gtactgcggc ccgaactggg tcgtctgctt gatctcgtca cgctccggat
     2581 ggaggacgcg ccggaggctg cgcggggaat tgctgccgag tttgtcgcgg gggttcttcc
     2641 ggaactcggc cacctgcatc cattcctggg gcgtcgggcc gatggagcct tcggggcgct
     2701 gccagttgtc gacacgccag atcttgtacc cctcgaagat gtcgttgccg gtgatcgggt
     2761 cgcgcgacag ttccgagacg ttatcccact gcagaatgac ccggcggtca ccctggggcg
     2821 ccgacaggta gatctcggag ttgggggccg ccggagcttc gggatcgccg aagagctcgg
     2881 gatcggtgtt ggtggccggc ggcggcgggg cggtcgttcc cacccagttg agcagcgcct
     2941 ccttgccacc gatgccggtg cagctgttgc agtcgtcatc gacgtaggcg gccgggatgc
     3001 aggttcggaa cgagatgttg acggtggtgc ccagtgtgtc gcacgggtcg tcccacatga
     3061 ccgggaagcg ggggtcggcg gcgatcaggc accgctcctt cccctcgaca cccgtctcgg
     3121 gattcccgtc ggcatcgagg tactggccgt tgaacacgcg ctgcgcgttg acggcgttgg
     3181 tgatcatccc ctgcagcccc tccccgatga cgaacgccgt ctggaatacc agcttgtcgc
     3241 ccgggtcgag ggtgaggaac ggcccggccg acagggtgaa ccggtaatcg tcgggcttgt
     3301 tggcgttgcg cgtgggaatt tcacgccgcg agagcaggtc gtaacgctcg gcgtccgtac
     3361 gcggatctcc ctgagggtag gccccggtcg aggaggtcca cttgactgtg gagagcccga
     3421 ctcggacggg tgccttgacg ccggccggat cggtggtgtg acccaggaac atgccgccga
     3481 aatagccggg gacatcgccg ccgacgatcg tggtgccgtt gtccggggca tcccagatgt
     3541 aggcgatatc catcgaaacc tgctggttgg cgcactggcc gctctggttc gggtcccggt
     3601 ccagcgtatc gacgcggacg aaaccgacca ggtcgtcggt ccagtagttg ttcatcgact
     3661 tggggcccgc gtcggcgtcc acgttgaaac cgatgaacat gtcctgtaaa cgctggtcgc
     3721 cgatgttggt gacctcgaac tcgaacccga caaactcgtt gatgtcgttg gtggcccact
     3781 ggaaggagcg ctggtgcacg aggaggccca gcgggcggtg ttcgttgagc tggttggtgg
     3841 cttcggtggt gttgtcgcgg tactcgcacg aatacatctg ctggccgatg gcggcgtagt
     3901 cctcgtcgac ctggccgtcg aggtcgtcgt ccttgccatt gtggaagtcc tcgtcctcga
     3961 ggccgtcgtc gtcgtcatcg gcgtcgaggg cctcggtatt gcgattgccg ccgcggatcc
     4021 cctcgtacga ctcgtagatg gtgtcgaccg ggagaaggga cgggcgaagc tcgaccggca
     4081 cggccgtcga gacgtggggc tcctggtcct cgccgatggc gccgagccac aggccgccca
     4141 ggtagagata ctcgccgccg cgccagccgg ccgacagttc gtcgatgaac gggttgccga
     4201 tgatcccgat gttggtgatc tggagcgtca gcagcccgac gttgtgcgtg aagatgtagg
     4261 gatcccgatt ggcgtagacc gcgcggatgt tgcgggcgcc gtttgccggg tcgttcaggt
     4321 cgatacgggc ctccgccccc ggaggaaggc acagcccccc cgccgtgatt cccatcgctg
     4381 ccgcgatcaa ccacgggcgg cggccacacc ggttcatggt cattcgtgcc tcctgtggcg
     4441 accagcccga gccgccagta tcgttgccgg ccgaagtcgg ccggatcagc tccctggacc
     4501 gcatcctaga acttcacgcc gacaccgaga cgcaggttgc gaccctcggt gaagactcgc
     4561 ggatcgttca ccggaatcca gtcctcggtg gcgtcgccgt tgacgtcgtc ccccaggtag
     4621 gcgccgccgg cgcgaccggt ctcggtgtag aacacgcggt agtcgttggt gttggtgccc
     4681 ggaggattcg gccagttgct cggctcgagg tcgttcaggt tccgggcgtc cagcacgttg
     4741 ttgccgcgga agaagacctt cacatcctgg ttgtagatgc tgaagtgctt ttccgcctgg
     4801 atatcgagct tggtggtgga cgggagccgg gcggcgttgg cgagcgacgg gtcgaccttg
     4861 cgctcgttgc ggctggtggg cgtgtaaggg aagcccgagc cgtacgacca gaccatgttg
     4921 accgcccact cggccggctg cgacagcagc acctgggccg agaaggtatg acgctggtcc
     4981 cagtcgagcg gctgctcgga gatcggcagg tagaggagct cctcgttccg ctggagattg
     5041 gggtcggagg ccacgccggt agccacgccg tagccgtacg acacgcctcc ggagaagttg
     5101 tggctgaacc gcttctcgag cgaaagctcg aaaccgcggg cggaggcgta gtcacggttc
     5161 acgtaggtcg gcaccaggtt cgggttgtca cccgacgaga gctgctcgat ggtgaggaga
     5221 ccgaagatgt ctttgaaata gaccgagaac tgcccgaaga tgtcctgcgt gaacatgtgc
     5281 tgcaggccgg cctggtacga gacggtggtc tcgttttcca ggttggggtt gccgcgaacc
     5341 tgggtggccg cgccgcggtt ctcgaagatg tattgacggt cgggaatctg gctgaaccgg
     5401 ccgtagtgga acgagaagac gtcacggtcg gatacggggt aggcgattcc gacgcggggc
     5461 gagaactggt tgcgcgagcg gtcctgcacc tcggagacgc cgagctggtc gcccaccgag
     5521 aagacgtcca cgcggccgcc gacgttgatg accatgcctt catggtccca gcggtcctgc
     5581 aggtagaacg ccccctcgtt gttgaagtag tggtactggc tgcggaagcc tccgacctgc
     5641 ccgttggcgt ttaccgacac gggaaagtcg atcgacagaa gtgacaggtc gttgtacacg
     5701 ccctcgatgc cgctcttgaa tttgtggcgc ccaacctggt gggtccagtc ggtcttgaag
     5761 ctgaacgtct gcgtgttgcg gtcggcgaac tgcggatagt cgccgttggt cgcgaagaag
     5821 cggctggtgg tgaagtcgat ggcattccgc cactgatccg ggtagcggcc gttgtactcc
     5881 cagggctcct gctctttcac gcggtagtcg tagtcgaacg catagcgcga caccttcacc
     5941 gtgtagaacg tggccggcga gagcgtgtgg ttccacacca gcttgacctg gttgaagttg
     6001 tcctccacgt tcggcgtgtg ctcggaggcg ttgtacggaa cgaaggtgga gtcctcggac
     6061 tcggcgctga agcggccgta ccgggtgacg atctgtccgg tggcccggat ggtgtcgaca
     6121 cgggtctgga cgaagccatt gaggctgaag atgtgggtat agatgtcgcg taccgagtgg
     6181 ttgttcaggt actcgagcgt gagcttggtg ctcggagccg ggcgccaggc cagcttcccc
     6241 tggtagttga aatcgttgtt ctggcggggg cccacccgaa tgaagtcgag gatcgtctgg
     6301 cgggggcgca cctcgttcgt cttgaggtag ccatccgaga aggtgccctg gaacgagaag
     6361 aagtaggtga ggtccttcac gccggtcggt ccgccgaagc ccacgttgag ccggtcgaag
     6421 ttgttgtagg tcttgtcggg ggcgccgaag tcgtcggtgg agtactcgac ggcgcctgag
     6481 aagctggcac cgccctcctg cgtggaaatg ttgatgacgc ccgactgtgc gttgccgtac
     6541 tcggcgtcga agccgccgag gaggatttcc gattcggcca cggcgttggt ggcgatgtcg
     6601 agcccgccgc cggccagggg atccgagacg cggatgccgt cgatcatgta ggccacttcg
     6661 ccggagcggc cgccgcggaa gtggagctcg ccggcctggg agacgacgcc ggccttcagc
     6721 gccacggcct cctggtaggt gtcgaccggg aggctctcca ggtcttcctt gccgatcttc
     6781 tgcttggtgg tgctgctctc gcgttccatg cgcgtacgcg cccccgtgac gttcacttcg
     6841 tcgatttcgg tggcgacggt ttccttgagc tcgaacttga tcgtggtggt ctggttggcg
     6901 ctgatgacca cgttcggccg gatcaggtcc tggaaaccga acttcttgac gaggaggcga
     6961 taggtgccga ccgggacgtt ccggatggtg aaattacccg cggcgtccgt cggtgcaccg
     7021 agaggagggg tgcttccctc cacctggacg atggcctggc tctccggttg gccgctgggc
     7081 ccgaccacga tgcccgtgat cgtgccggcg gcggcggcat gggccaggtt cgccagcgag
     7141 aggccggcga gcagaacgat gagtatggca agccctgacc ggaagagagc aatacccctc
     7201 gacatcgatg caggctcctt tcgggcaatg tcagatagtc gacaagctgt tgcaggtctt
     7261 gcgactatag gaccgtgaca cttgagtgtc aacgaatcgg cgctcccgcc aggggtaggg
     7321 aaaaacccgg atggtcggaa agctccacga cgtcccgttc acggacggga aactccggca
     7381 agcgagactc agacaggcag caggcagcca ccggattcgg tggcaggagg ggagtcgttg
     7441 gaagagggct ttcgcccacc tccttcgacc tgatcggaat atatcggctg cgaggccggg
     7501 aacgcaagat caaaatagaa cacgggcgta
//
LOCUS       DASVOD010000466        13728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_292125, whole genome shotgun sequence.
ACCESSION   DASVOD010000466 DASVOD010000000
VERSION     DASVOD010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13728
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292125"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VF720_10515"
     CDS             complement(<1..283)
                     /locus_tag="VF720_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7879834.1"
                     /translation="MKRLLKLFGVVMLAGLFLAGCQEDDNTDNGPGNGGGDGTSGVPA
                     EIVIARTFPALLANGVDEVEVKATVVDGQGRGLGNVGVFFTTNRGTIEPF"
     gene            400..1440
                     /locus_tag="VF720_10520"
     CDS             400..1440
                     /locus_tag="VF720_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7879835.1"
                     /translation="MGILRALMTASNRAIETSRLGREYRIRNAKPPDPTTLTALRDVD
                     LHVERGELFGLLGPNGAGKTTLIKILTTLLAPTTGIARIAGFDVASQSREIRSRINMV
                     SGGESSGYGLLTVEENLWMFSQFYGMRSGEARRRIGELLEVVGLADRRRTKCSDLSTG
                     LRQKMNIVRGFLTDPEVLFLDEPTLGLDVGASRDVRDFVRRWMAEDPRRTLLLTTHYM
                     VEADDLCHRVAIINRGELKACDTPANLKRRLATGVRYRMSVSPPEGTDLDFLERMPGV
                     VRSDSTLSDGLMHLDLTLAADDALGTLLAAFDGRGIRLTNLGKHEATLEDVFVDVVGE
                     SMEAAEQPHGGD"
     gene            1427..2290
                     /locus_tag="VF720_10525"
     CDS             1427..2290
                     /locus_tag="VF720_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7879836.1"
                     /translation="MAATESTAPGRAAPTRDTGLRLFLRTVRGRAYPRVIGSMREKSW
                     IFFEIFLPLLTVMAYVWVYRALGAPDAYTGFVLLGGAMTAFWANVLWAMSSQLYWEKE
                     QGNLALYIMSPTTLMALLLGMAIGGLLSTAARAAAIVAIGALFFHVTFEPGRLPELAL
                     VFLVTMTALYGMGMMLASVFLLLNREAWHLANLAQEPIYLLSGFFFPVTALPTALVAG
                     SSIVPLTLGMDAMRQLLFPAASIRGFLPVPVELGIVAFLAVFYLIGARRLLDFMERLA
                     IEDGRLTESRK"
     gene            2287..3156
                     /locus_tag="VF720_10530"
     CDS             2287..3156
                     /locus_tag="VF720_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7879837.1"
                     /translation="MSAPAVRVPGVWHTFLTAARLGWKIESNWTDPFLFLVYSILKPL
                     SHAAILVVMIMVVSGGRTEGALFNYIVIGNACYTWATSVIVGVSFGVLDDRERYKTLK
                     YLCLAPIPLPLYLIGRGAAQFLMGCIAVALTLLLAALVFHLPIRPGEIRWGWLLASLL
                     FGIVSLALIGLLVGGMTLLMAHQVEVVGYAVAGSLYLVSGAIFPLEVLPSWLRWIGYG
                     LPLTWWLELTRRALVGNAAAASPSMAAFGDASVLAILAGQTVVLAVVATLGWKWAYHR
                     ARERGLLDKVTNY"
     gene            3207..4424
                     /locus_tag="VF720_10535"
     CDS             3207..4424
                     /locus_tag="VF720_10535"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7879838.1"
                     /translation="MLAILLAAVLLIGDRHYDGISDNLSGPVRILVEDGRITAMGPDI
                     EAPAGAERIDLEGMTLLPGFIDCHTHLSYLWRDTTAAPNFLPDYLGAPALVAFEAARN
                     ARTTLEAGFTTVREMGCVDGTDRVLAEAVRRGLVLGPRIVTAGALYPPSGGGRTDIEW
                     PKDGTVRDAASITARTRDYIGDGCDWIKIYATGGTYDDTTGVPLFTGAEIAAAVEVAH
                     PRGRWVAAHAMGLEGARRAVQAGVRSIEHGSRLDDAVAREMARRHTWLVPTLYHLEWY
                     ATHGKALGYGPGYAERLAALTKIQVESIGRARKAGVAIAAGSDAVYTMHGQNAREIVW
                     LTRAGLTPIEALRAATGTAARLLGLENEIGRLAPGMAADIVAVPGDPTADIEVVSRVG
                     FVMAAGKIIRRNP"
     gene            4594..5304
                     /locus_tag="VF720_10540"
     CDS             4594..5304
                     /locus_tag="VF720_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX7879839.1"
                     /translation="MPFSPRRIAVALGLAFGCGAGVVAGSALASDHQDTPEVELNPKC
                     DINDVYAFPGSSPSRICLVMTTSSPIAAGTANPSFDPDKLYQIKVDNTGDAVEDLVLQ
                     FIFTGTGSGQMVALFGPAAPVATGARNRIINANATVSGSINSNLGAGDNVQLFAGLRD
                     DPFFLDLEQFFRLLPDRRPATGPLAVANPIDPPAWRDPGVDYLRNYNTLAIVVELPLE
                     RLTVGGNAEFGVWGTISR"
     gene            5349..5903
                     /locus_tag="VF720_10545"
     CDS             5349..5903
                     /locus_tag="VF720_10545"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX7879840.1"
                     /translation="MTDFKRILSALLIAVLVTMSGGCSDDDDDNGGTTSVNLNREYVQ
                     IERLGNPLVSEVFLAKRQHGTHNASIPSQDVSLYKQEMEDFVEGFGRPPIVGTTLTGV
                     LLPDMLIVDSSKSTTSAGWLSNALSGGYGGRKLTDDVVDTGLSAIFGTALSATGAIPA
                     LASDNVDANDKSFGNTFPYLATPH"
     gene            6020..7057
                     /gene="hemE"
                     /locus_tag="VF720_10550"
     CDS             6020..7057
                     /gene="hemE"
                     /locus_tag="VF720_10550"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX7879841.1"
                     /translation="MNDRFLRACRKQPVDVTPVWIMRQAGRYLPQYMAIRKGTDFLTF
                     CKSPKLLAQAAFDAVDILGVDAAILFSDILLPLEAMGLKLIFEESHGPKILDPVRSGA
                     DIARLTLPDPGRTMPFVGEAIERTLERLNGSVPLIGFAGAPFTLASYAIEGGGSKNYA
                     KTLGWLHRDPESFDKLLDLMTMTIIRHLEFQVRIGVMAVQLFDSWGGWVSADLYREHM
                     VPRLARIVAAIQPLGVPIILYVNGSTHLLEAMAETGADVLSVDWRLPLDEVRRRVGPG
                     PALQGNLDPCALYAPPDVIAEKVRAILAAAGPSGHIMNLGHGILPDAPVTGARAMVAA
                     AHAVRPLAAQP"
     gene            7054..8451
                     /gene="hemN"
                     /locus_tag="VF720_10555"
     CDS             7054..8451
                     /gene="hemN"
                     /locus_tag="VF720_10555"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX7879842.1"
                     /translation="MTPRDRTVAPPVFDATILARYDRSGPRYTSYPTAPHFNDTFGPA
                     DYLAAVGKAGQAPGREASFYFHLPFCRRVCYFCACNTIFTNDRNTGRAYTDDLKREMD
                     LVLDAMRHRPAVRQLHWGGGTPTFSPPEILDELGRAIHERLTFAPGAEVSVEVDPRET
                     TPAHLDVLAGHGFNRISLGVQDFDADVQKAVNRVQPWEMTKGVVDAARARGFGSVNID
                     LIYGLPRQTPAVFEATLDRLLELDPDRVALFNFAYLPEMVRHQRVIKPEQLPPPADKL
                     AMFRRGIERLTGAGLVFIGMDHFARADDELALALEAGTLGRNFQGYTTHAGLDLFGFG
                     VSAIGQVGDTYAQNQKSREAWRAALDAGVAPVARGITLMRDDEIRRAVIMELMSRFAL
                     DREKLARRFGIDVDDYFADEFRRLSALEEDGLVTVTPAAITVTAAGRLLVRNVAMIFD
                     AWLAGEAAPRYSRTV"
     gene            8459..9463
                     /gene="hemH"
                     /locus_tag="VF720_10560"
     CDS             8459..9463
                     /gene="hemH"
                     /locus_tag="VF720_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX7879843.1"
                     /translation="MIDTTALILMTYGTPDGPAAVEPYLYNIFTDPDIIDLPWYMKPF
                     RKGLARKIARGRTEEVQHNYARLPGGRSPLNELTAAQGEALVKALEGDGSFESHLGMR
                     YWAPRTEETLERLLARGIRKFMLVPMYPQYARATTGSSITDFRQAARKLGVTRSIEVR
                     AVCCFPDNAGMIDAMAADIVAATKDLSPAEFLATPLLFSAHGLPQSMVDKGDPYQEHV
                     ERTARAVIERLGGHPDATVCYQSRVGKQVWLQPYTDEILQALIARKVPRVIVYPVAFI
                     TEHSETLFELDLLYGDDLRAAGIDYRRVPTVGTSPAFIDGLAGEIRRALAGPLLRIDG
                     "
     gene            9460..10812
                     /gene="hemG"
                     /locus_tag="VF720_10565"
     CDS             9460..10812
                     /gene="hemG"
                     /locus_tag="VF720_10565"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX7879844.1"
                     /translation="MNDQRAAEDAPTLVVGGGIGGLTLAFRLATSGRAVRLIEPAERP
                     GGAITTDVSNGWTFELGPNTVVAGEPAIDALIEDAGLSGELLTADRVGARRFVVRNGR
                     MIALPRSPLEFVRSPILSPAGRLRLLAEPLVPRRRSSGEESIADFIRRRLGGEALDWL
                     AAPFVSGVYAGDASRLSVRHALPRLAAWEADHGSLFRGMIAARRQGGAPRGAKLVSFQ
                     GGLATLPRRLADRLGDRIIRGTVDTLHRQGEAWIAGCSLPDGSTRTISARRVVLAVSA
                     AQAARLLDEPELANLPVAGVRVAAFGFKRQDVAHPLDGFGVLVPRREGRRLLGALFPS
                     SLFPGRAPDGFVGITAMAGGVNDPAVLALAADEFVAHVLGELGRLLGISAPPVHVITR
                     SWREAIPQYELGHQRWLDLAERCERDRPGLHLLGSWRGGVSVPDRIRAATELAAHLSR
                     "
     gene            10832..11875
                     /locus_tag="VF720_10570"
     CDS             10832..11875
                     /locus_tag="VF720_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879845.1"
                     /translation="MIVRAALASLVALACWSGPARTPSGHHDDRIRGVALAEIHPGRP
                     LSAYRHALDDIRSLGADWVLLPVFGYVDSASSPGIDTDWERGYSMATYRRRVAEVVRE
                     ADERDLAVALVPYLLLRTGQEQDWRGTLAPDDWPAWFRSYRAFLDGWISLAVSARVPL
                     LAVGAELASSEAREAEWRSLIAHVRSRYQGRLFYSCNWDHYEETTFLDALDVVGVSGY
                     FSPDHDVTATDAALDAMWMEVRRAMLTWQERMDRPFFFTEVGCPSIKGAASHPWNYQA
                     PGPADPAEQARALAAFCRAWHDRPEPEGVFYWNWSPFRGGISDRSYSIRRKPAADIVR
                     GCLARPATPAPAP"
     gene            12061..12387
                     /locus_tag="VF720_10575"
     CDS             12061..12387
                     /locus_tag="VF720_10575"
                     /inference="COORDINATES: protein motif:HMM:NF015959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVG family protein"
                     /protein_id="HEX7879846.1"
                     /translation="MDITEVRIKLCDNDPVRAIASITFDDCFVVKGIRILHVHDRFIV
                     CMPSRPRKDGRHQDVAHPINAPTRDRFNRHILDAYEDELGRFERGEGPSHHPDDDQGP
                     QGIYPA"
     gene            12384..12629
                     /locus_tag="VF720_10580"
     CDS             12384..12629
                     /locus_tag="VF720_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879847.1"
                     /translation="MKSVLFWITAAITMVVSVAVSLVYFNLAMNLIPAAFLSQFNRAA
                     AQTAFIFWGIGLGLAVAVLEGLLFWILPRVLPRRSAA"
     gene            12928..>13728
                     /locus_tag="VF720_10585"
     CDS             12928..>13728
                     /locus_tag="VF720_10585"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX7879848.1"
                     /translation="MIGRRSRWLLVVLGLTLALTPLVDGVLAVRSVEAAGTANSTTRS
                     STKSTTKKATPAKSTSSKKSTTSTKKTGGKSTARTSTSVKKSSGKSAGGKKATTKRTV
                     SRRNRKSARRPAPVVAFDPARPATLRAESAVVLDRRTGHVLWGKNADETRPVASLTKL
                     MTALVVSESPLAMDDSITVTMDDVTGAGRSHVRAGNRVSIGDLYHCSLISSDNAATRA
                     LARATGMSSTAFVARMNQRARELGMEHSHYVETTGLDAGNVSTARDQAR"
BASE COUNT         2132 a         4854 c         4431 g         2311 t
ORIGIN      
        1 cgaacggttc gatcgtcccg cggttggtgg tgaagaacac gcccacgttc ccgagtccgc
       61 gtccctgccc atccaccacg gtggccttca cttcgacctc gtccacgccg ttggcgagga
      121 gtgcgggaaa ggtgcgcgca atgacgatct cggccggcac cccggaggtg ccgtcgccgc
      181 caccgttgcc gggcccgtta tccgtgttgt catcctcctg gcagccggcc aggaagaggc
      241 ctgccaacat cacaaccccg aacagcttca acaggcgctt catcgcgttc tccacggtcg
      301 gtgagcggaa cagcgggccg cctcgaggcg catccccacc cggttcaaac ggcacaagtt
      361 ataaaggatc aggggcccga gggccagcag gatgcgagct tgggtatact ccgcgccctc
      421 atgaccgcct ccaatcgcgc catcgagacc agccgactcg gtcgcgagta ccggatccgg
      481 aacgcaaagc cgcccgatcc gaccactctg acggccctcc gcgacgtgga tctccacgtg
      541 gaacggggcg agttgttcgg actactcggg cccaacggtg ccggcaagac caccctcatc
      601 aagatcctca ccacgctcct ggcgcccacc accggcatcg cccgaatcgc cgggttcgat
      661 gtcgcctccc agagcaggga aattcgttcc cggatcaaca tggtttccgg gggcgaatcg
      721 agcggttacg gcctcctgac cgtcgaggag aatctctgga tgttctccca gttctacggc
      781 atgcgatcgg gtgaggcccg tcgacggatc ggtgaactgc tggaggtggt tggacttgcg
      841 gaccggaggc gtaccaagtg ctcggacctt tccaccgggc tccggcagaa gatgaacatc
      901 gtgcggggct tcctgaccga ccccgaggtg ctcttcctcg acgagccgac cctggggctc
      961 gacgtggggg cctcacgcga tgtccgcgac ttcgtccggc gctggatggc cgaggacccg
     1021 cgccgcaccc tcctcctcac cactcattac atggtggaag cggacgacct ctgccaccgg
     1081 gtggccatca tcaaccgggg agagctcaag gcgtgcgaca cgccggccaa cctgaagcgc
     1141 cgccttgcga ccggcgtccg gtaccggatg agcgtgtcac caccggaggg tacagacctc
     1201 gactttctgg agcggatgcc cggcgttgtc cggtcggact cgacgttgag cgacggcctg
     1261 atgcatctcg acctgacact tgcggcggac gatgctctcg gcacccttct cgccgcgttc
     1321 gacggacgag gcatccggct gaccaatctc gggaagcacg aggccacact cgaggacgtc
     1381 ttcgtcgatg tggtcggcga aagcatggaa gccgcggagc agccgcatgg cggcgactga
     1441 gtcgaccgct cccggccggg ccgctcccac ccgggacacc ggcttgcgcc tcttcctgcg
     1501 cacggtgcgc ggtcgcgcct atccgcgcgt gatcgggagc atgcgggaga agtcgtggat
     1561 ctttttcgag atcttcctcc cgctgctgac ggtgatggcc tacgtctggg tgtaccgcgc
     1621 cctcggcgcg cccgatgcgt acacgggttt cgtcctgctc ggcggcgcca tgaccgcctt
     1681 ctgggccaac gtcctctggg cgatgtcgag ccagctctac tgggaaaagg aacagggaaa
     1741 cctggcgctc tacatcatgt cgccgaccac gctcatggcg ctgctgctcg gcatggccat
     1801 cgggggcctc ctctccaccg ccgcccgggc ggccgccatc gtggccatcg gggccctttt
     1861 cttccacgtg acattcgagc cggggcggct gccggagctg gcgctcgtct ttctcgtcac
     1921 catgacggcg ctttacggca tggggatgat gctggcctcg gtcttcctgc tcctcaaccg
     1981 cgaagcgtgg caccttgcca acctggcgca ggagccgatc tacctcctgt cgggtttctt
     2041 ctttccggtg acggcgcttc ccaccgcgct ggtggcgggc tcgtcgatcg tgccgctcac
     2101 gctcggcatg gacgccatgc gccagctcct ctttccggcc gcctccatcc ggggctttct
     2161 cccggtgccg gtcgaactgg gcatcgtggc cttcctggcg gttttctacc tgatcggcgc
     2221 ccgccggctg ctcgacttca tggagcgcct ggccatcgag gacggccgcc tgaccgagag
     2281 ccgcaagtga gcgcgccggc ggtccgtgtc cccggtgtct ggcacacgtt cctgaccgcc
     2341 gcgcggctgg ggtggaaaat cgagtccaac tggaccgatc ccttcctctt cctcgtctat
     2401 tcaatcctga agcccctctc gcacgccgcc atattggtcg tgatgatcat ggtcgtgtcg
     2461 ggggggagga ccgagggggc gctcttcaac tacatcgtga tcggcaacgc ctgttacacg
     2521 tgggccacgt cggtcatcgt gggcgtgtcg ttcggcgtgc tggatgaccg ggagcggtac
     2581 aagactctca agtacctctg cctcgccccc atccccctgc cgctctacct catcgggcgc
     2641 ggcgcggccc agttcctgat ggggtgcatc gcggtggcac tcacgctcct gctggcagcg
     2701 ctcgtgttcc accttcccat ccgccccggc gagatccgat ggggatggct cctggcatcg
     2761 ctcctcttcg ggatcgtgtc gctcgcgctc atcggcctcc tggtcggcgg catgaccctg
     2821 ctcatggccc accaggttga ggtggtgggg tacgcggtgg ccggctcgct ctacctggtg
     2881 tcgggcgcga tttttccgct cgaggtactt ccgtcgtggc ttcgatggat cggctacggg
     2941 ctgccgctta cctggtggct cgaactgacg cggcgggcgc tggtcggaaa cgccgccgcc
     3001 gcatccccct ccatggccgc tttcggcgat gcgagcgtgc tcgccatcct ggccgggcag
     3061 acggtcgtgc tggcggtggt ggccacgctc ggctggaagt gggcctatca tcgtgcccgc
     3121 gagcgcgggc tcctggacaa ggtgaccaac tactgacgac gcggccacgc gatgccgccg
     3181 accaacttca acggacgagg tagcccatgc tcgccatcct gctggccgcc gttctgctca
     3241 tcggggatcg tcactacgac ggaatctccg ataacctgtc gggccccgtg cgcatcctgg
     3301 tcgaggacgg ccggatcacc gcgatgggcc ccgacatcga agcgccggcc ggcgcggagc
     3361 ggatcgacct cgaggggatg acgctcctgc cgggcttcat cgattgtcac acccacctct
     3421 cctacctctg gcgcgatacg acagccgcgc cgaacttcct ccccgattac ctgggtgcgc
     3481 ccgcgctggt ggccttcgag gcagcccgga acgcgcgcac caccctcgag gccggcttca
     3541 ccacggtccg cgagatggga tgcgtggacg gcaccgaccg cgtgctggcc gaggcggtgc
     3601 gacgcgggct cgtcctcggg ccgcggatcg tgacggcggg cgcgctctat ccaccttcgg
     3661 ggggcggccg cacggacatc gagtggccga aggatggcac cgtgcgcgat gccgcctcga
     3721 tcacggccag gacgcgcgac tacatcggcg acggctgcga ttggatcaag atctacgcca
     3781 ccggcggcac gtacgacgac acgacgggtg tgcccctctt caccggggcg gagatcgccg
     3841 ccgccgtgga ggtggcccat ccacgcggcc gctgggtggc ggcgcacgcg atggggttgg
     3901 agggagcgcg gcgggcggtc caggccggcg tccgctcgat cgagcacggc tcgcgcctcg
     3961 acgacgccgt ggccagggag atggcgcggc gccatacctg gctcgtcccc acgctgtacc
     4021 acctcgagtg gtacgccacc cacggcaagg ccctcggtta cggaccggga tacgccgaac
     4081 gcctcgcggc gctcacgaag atccaggtcg agtcgatcgg acgcgcgcgc aaggccggcg
     4141 tggcgatcgc ggcgggttcg gatgccgtct acacgatgca tggccagaac gcccgcgaga
     4201 tcgtctggct cacccgcgcg gggctcactc cgatcgaggc gcttcgcgcc gccaccggca
     4261 ccgcggcccg gctgctcggc ctggagaatg aaatcggccg cctcgctccc ggcatggcgg
     4321 ccgacatcgt ggccgttccc ggcgatccca ctgccgacat cgaggtggtg agccgcgtgg
     4381 gcttcgtcat ggccgcggga aagatcatcc gccgcaaccc gtagccgccg gtccgctaag
     4441 ggtttccctc attcggcctt ccgacacccg atgctagcct cggcaggcag attccccaat
     4501 ccgcggccgc acgctcctcg tatatggctc ggacatgacg gcggcaggct cgtggccgtt
     4561 acgcccggcc gtcaaatgga caaggaggtt tgcatgccgt tttccccccg ccggatcgca
     4621 gtggccctcg gcctggcgtt cggttgcggt gcgggcgtgg tcgccggctc cgccctcgcc
     4681 tcggaccacc aggacacgcc cgaggtggaa ctcaacccga agtgcgacat caacgatgtc
     4741 tacgcctttc cgggatcgag cccatcgcgc atctgcctcg tgatgacgac gagctcgccg
     4801 atcgcggcgg gtaccgccaa cccttccttc gatccggaca agctctacca gatcaaggtg
     4861 gacaatacag gtgacgccgt ggaggacctc gtgctccagt tcatcttcac ggggaccggc
     4921 tcgggccaga tggtggcgct cttcggcccc gcggcgccgg tcgccaccgg ggcgcgcaac
     4981 aggatcatca atgccaacgc caccgtgtcc ggaagcatca actcgaacct cggcgccggc
     5041 gacaacgtgc agctcttcgc cggcctccga gacgatccct tcttcctcga cctcgagcag
     5101 ttcttccgcc tcctccccga ccgccggccg gccaccgggc ccctggcggt ggccaatccg
     5161 atcgatccac ccgcctggcg cgacccggga gtcgactacc tcaggaacta caacacgctg
     5221 gccatcgtgg tggaacttcc gctggaacga ctgaccgtcg gcggcaatgc cgagttcggt
     5281 gtctggggca cgatcagccg ctgacgcaag tcggcccctg atccaacgcg agatcgagga
     5341 gaaacgagat gacggacttc aagcgaatcc tgtcggccct gctgatcgcc gtgctcgtca
     5401 ccatgagcgg cggctgcagc gacgacgacg acgacaacgg cggcacgacc agcgtgaacc
     5461 tgaaccgcga gtatgtgcag atcgagcggc tcggcaatcc actggtgagc gaggtcttcc
     5521 tggcgaagcg acagcacggc acgcacaacg cgtcgatccc ctcccaggac gtatcgctct
     5581 acaagcagga gatggaagac ttcgtggagg gattcggccg cccccccatc gtcggcacca
     5641 ccctcaccgg cgtgctgctt cccgacatgc tgatcgtgga cagctcgaag agcacgacct
     5701 cggccggctg gctcagcaac gcgctttcgg gcggctatgg tgggcgcaag ctcaccgatg
     5761 acgtggtcga taccggtctt tcggccatct tcggcacggc gctcagcgca accggggcca
     5821 tcccggccct ggccagcgac aatgtcgacg ccaacgacaa gtcgttcggc aacacgtttc
     5881 cgtacctggc aacccctcat tagagagggc ttcggcaaga cttccgcccc ggccccgccc
     5941 acccggcggg gtcggggctt ttttcgttcc gcggcgggcc ctttttcacc cgcgtgccgg
     6001 gcgggtatcc tctccggcaa tgaacgatcg attcctgagg gcctgccgga agcagccggt
     6061 ggacgtgacg ccggtctgga tcatgaggca ggccggccgc tacctccccc agtacatggc
     6121 catccgcaag ggcaccgact tcctcacctt ctgcaagtcg cccaagctcc ttgcccaggc
     6181 cgccttcgac gcggtggaca tcctcggtgt cgacgccgcc atcctcttct cggacatcct
     6241 cctgccgctc gaggcgatgg ggctcaagct catcttcgag gagtcccacg gcccgaagat
     6301 cctcgacccg gtccgctcgg gtgctgacat cgcccgcctc accctccccg atcccggccg
     6361 cacgatgccg ttcgtcgggg aagccatcga acgcacgctc gaacggctga acggatcggt
     6421 gccgctgatc ggattcgcgg gggcgccgtt cacgctggcc tcgtacgcca tcgagggggg
     6481 gggctcgaag aactacgcga agacgctggg gtggctccat cgcgatcccg agtcgttcga
     6541 caagctgctc gatctcatga cgatgaccat catccgccat ctcgaattcc aggtccgcat
     6601 cggcgtcatg gccgtgcagc tcttcgactc atggggcggg tgggtgtcgg ccgacctcta
     6661 ccgcgaacac atggtgccgc ggctcgcgcg gatcgtggcg gccatccagc cgctgggcgt
     6721 gccgatcatc ctctacgtga acggttcgac ccacctgctc gaggcgatgg ccgaaaccgg
     6781 tgcggatgtc ctctcggtcg actggcggct gcccctcgac gaagtgcggc gacgcgtagg
     6841 ccccggtccc gcgctccagg gaaacctcga tccatgcgcg ctctacgcgc cgcccgacgt
     6901 gatcgccgaa aaagtccgcg ccatcctggc cgcggccggc ccgtcgggac acatcatgaa
     6961 cctggggcac ggcatccttc ccgatgcgcc ggttacgggc gcccgggcca tggtggcggc
     7021 cgcgcatgcc gtgcggccgc tggcggccca gccatgacgc cgcgcgaccg taccgtcgcg
     7081 ccgccggttt tcgacgccac catcctggcc cgatacgacc gctccgggcc gcgctacacc
     7141 tcgtacccga ccgccccgca tttcaacgac accttcggcc ccgccgatta cctggccgcc
     7201 gtcggcaaag ccggccaggc accgggacgc gaggcctcgt tctacttcca tctcccgttc
     7261 tgccgccgcg tctgctactt ctgcgcctgc aacaccatct tcaccaacga ccggaacacc
     7321 gggcgagcct acaccgacga cctgaagcgg gagatggacc tggtcctcga cgccatgcgc
     7381 catcggccgg ccgtccgcca gctccactgg gggggcggca cgcccacctt cagccctccg
     7441 gagatcctcg acgaactggg acgggccatt cacgagcgcc tgaccttcgc gcccggggcc
     7501 gaggtgtcgg tggaggtcga tccacgcgag accacgccgg cccatctcga cgtcctggcc
     7561 ggccacggtt tcaatcggat cagcctcggc gtccaggact tcgacgccga cgtgcagaag
     7621 gcggtgaacc gcgtccagcc ctgggagatg acgaaagggg tggtggacgc cgcgcgggcg
     7681 cgcggcttcg gaagcgtcaa catcgacctg atctacggcc tgccgcgaca gacgccggcc
     7741 gtcttcgagg caacgctcga ccggctgctc gagctcgacc ccgatcgcgt ggcgctcttc
     7801 aacttcgcct acctgcccga aatggtccgc caccagcggg tcatcaagcc cgagcagctc
     7861 ccgccaccgg ccgacaagct ggccatgttc cgtcgcggga tcgagcggtt gaccggggcg
     7921 gggctcgtct tcatcgggat ggatcacttt gcccgcgcgg acgacgaact ggccctggcg
     7981 ctcgaggcgg gcaccctcgg ccgcaacttc caggggtaca cgacgcacgc ggggctcgac
     8041 ctcttcggat tcggggtgag cgccatcggc caggtcggag acacctacgc ccagaaccag
     8101 aagtcacgcg aggcctggcg ggccgcactc gatgcgggtg tcgctccggt ggcccgcggg
     8161 atcacgttga tgcgcgacga cgagatccgc cgggcggtca tcatggagct gatgagccgg
     8221 ttcgcactcg acagggagaa gctggcgcgg cgattcggca tcgacgtgga cgactacttc
     8281 gccgacgaat tccggcgcct ctcggcgctc gaggaggacg gcctcgtgac cgtcacgccg
     8341 gctgccatca cggtgaccgc cgcgggacgc ctgcttgtcc gcaacgtggc catgatcttc
     8401 gacgcctggc tggccggcga agctgccccc cgatactctc gtaccgtcta ggagaaccgt
     8461 gatcgacacg accgcactca tcctcatgac gtacggcacg ccggacggac ccgcggccgt
     8521 cgagccttac ctgtacaaca tcttcaccga cccggacatc atcgaccttc cctggtacat
     8581 gaaaccgttc cggaaagggc tggcgcgcaa gatcgccagg ggacgcaccg aagaagtgca
     8641 gcacaactac gcccgccttc ccggcggccg ctccccgctc aacgagttga ccgcggcgca
     8701 gggagaggcg ctggtcaagg cgctcgaggg cgatggatcc ttcgaatcgc acctcggcat
     8761 gcgttactgg gcgccacgca cggaggaaac gctcgaacga ctcctggcca gggggatccg
     8821 caagttcatg ctggtgccga tgtacccgca gtatgcccgc gccacgaccg gctcgtcgat
     8881 caccgacttc aggcaggccg cccgcaagct gggcgtgacg cgctccatcg aggtccgcgc
     8941 ggtctgctgc ttcccggaca acgccgggat gatcgacgcc atggccgccg acatcgtggc
     9001 ggccacgaag gatctttcgc cggccgagtt cctggccacc ccgctgctct tcagcgcgca
     9061 tggcctgccg cagtcgatgg tcgacaaggg agatccctac caggaacacg tggagcggac
     9121 ggcccgggcg gtgatcgagc gcctcggcgg ccacccggat gccaccgtct gctaccagag
     9181 ccgcgtgggg aagcaggtgt ggctccagcc gtacaccgac gaaatactgc aggcgttgat
     9241 cgcccgcaag gtgccgcgcg tgatcgtcta tccggtggcc ttcatcaccg aacactcgga
     9301 gacgctcttc gagctcgacc tcctgtacgg agacgacctc cgggcggccg gcatcgacta
     9361 ccggcgcgtg ccgacggtcg gaacgagccc ggccttcatt gacgggctgg ccggggagat
     9421 tcgacgcgcg ctggccggac cgctgctgcg catcgacgga tgaacgacca gcgagcagcc
     9481 gaagacgcgc cgacgctggt cgtcggcggc ggcatcggcg gactcacact cgcatttcgc
     9541 ctggccacct ccggccgcgc ggtgaggctg atcgaaccgg ccgaacgtcc gggcggggcc
     9601 atcacgaccg atgtgagcaa cggctggacg ttcgagctcg gtccgaacac ggtggtggcc
     9661 ggcgagccgg ccatcgatgc cctcatcgag gatgccggcc tctcgggcga actcctgacg
     9721 gccgatcggg tcggcgcgcg ccgattcgtg gtgcgcaacg ggcggatgat cgcgctgccg
     9781 cgctcgcccc tcgagttcgt gcgatcaccg atcctctccc cggccggaag gctccgcctg
     9841 ctggccgagc cactggttcc gcgccgcagg tcgtccgggg aggaatccat cgccgacttc
     9901 atccgacggc ggctgggtgg cgaggcgctc gactggctgg ccgcaccgtt cgtttccggc
     9961 gtctacgcgg gcgatgcctc gcggctgtcg gtgcgccatg cgctgccgcg cctcgcggcc
    10021 tgggaagccg atcacggaag cctgttccgg gggatgatcg ccgcgcgccg gcaaggaggc
    10081 gccccgcgcg gcgcgaaact ggtctcgttc caaggcggcc tggccaccct cccccgccga
    10141 ctcgcggatc ggctcggaga tcgcatcatc cgtggcacgg tggacacgct acatcggcag
    10201 ggcgaagcct ggattgccgg ttgttccctc cccgacggat cgacgcgcac gatctccgcg
    10261 cgccgggtgg tcctggccgt ctccgccgcc caggccgccc gcctcctcga tgaaccggag
    10321 ctggcgaacc ttcccgtggc cggcgtgcgc gtggccgcct tcgggttcaa gcggcaggat
    10381 gttgcacatc cgctcgacgg gttcggtgtg ctcgttccgc gccgcgaggg gcgtcgcctg
    10441 ctcggcgccc tcttcccctc gtcactcttt ccggggcgtg cgcccgatgg cttcgtgggc
    10501 atcaccgcca tggccggcgg cgtgaacgat cccgcggtgc tcgcgctggc cgccgacgaa
    10561 ttcgtggcgc acgtgctcgg ggagctcggt cgcctgctcg gcatcagtgc gccgccggtg
    10621 catgtcatca cacgtagctg gcgtgaggcc atcccccagt acgagctcgg tcaccagcgc
    10681 tggctcgacc tggccgaacg gtgcgagcgc gatcggccgg gacttcacct gctcggaagc
    10741 tggcggggtg gcgtgtccgt gcccgaccgg attcgggccg cgaccgagct cgccgcacac
    10801 ctctcgcgat gatgctggcc cgatcggttc gatgatcgtc cgagcggcgc tggcctcgct
    10861 cgtggcactc gcctgctgga gcggcccggc gcgcacgccg agcggtcatc acgacgaccg
    10921 gattcggggc gtggcactgg ccgagatcca cccgggccgg ccgctcagcg cctaccgtca
    10981 cgcgctggac gatatccgct ccctcggcgc cgactgggtt ctgctgccgg tcttcgggta
    11041 tgtcgactcc gcctcctccc cgggaatcga caccgactgg gagcgcggct attcgatggc
    11101 gacgtatcgc aggcgtgtgg ccgaggtcgt gcgcgaagcc gacgaacgcg acctggccgt
    11161 ggccctcgtt ccctacctcc tcctccgcac ggggcaggag caggactggc gtggaactct
    11221 cgctcccgac gactggccgg cctggttccg ctcataccgg gcattccttg acggctggat
    11281 ttccctggcc gtgtccgcgc gcgtgccgct gctcgcggtg ggcgccgagc tggcctcgtc
    11341 ggaagcccgg gaagcggagt ggcgttccct gattgcgcat gtccgttcac gctaccaggg
    11401 tcgacttttc tattcctgca actgggatca ctacgaggag accaccttcc tcgacgccct
    11461 cgatgtcgtc ggcgtctccg gctatttctc cccggaccac gacgtgacgg caactgatgc
    11521 cgcgctcgac gcgatgtgga tggaggtgcg gcgcgcgatg ctcacctggc aggagcggat
    11581 ggatcgaccc ttcttcttca ccgaggtggg atgtcccagc atcaaggggg cggcgagcca
    11641 cccgtggaac taccaggccc cgggccccgc cgatcccgcc gagcaggcgc gcgcgctcgc
    11701 tgcgttctgc cgggcctggc acgaccgccc ggagccggaa ggtgtgttct actggaactg
    11761 gagtccgttc cgtggcggaa tttccgatcg ctcctactcg atccggagaa agccggcagc
    11821 cgacattgtt cgcggctgcc ttgcacggcc cgcaactccg gcaccagcgc cgtgacgtcg
    11881 tacgggcggg cgctcctcgg aattccccgg ccgaggataa ccgggccggc gcgaaagtgt
    11941 ccacgcgcgc cgctttcgca agaaattcga tggtattcgc ttgctgtcat caacgcgctg
    12001 cgatacggtc ccgctgctcg tgaagtggaa gtggttgtga cgaaggaggg ggttcgccac
    12061 atggacatca cagaggtccg catcaagctg tgtgacaatg atcctgttcg cgccatcgcc
    12121 tcgatcacgt tcgatgattg tttcgtcgtg aagggaatcc gcatccttca cgtgcacgac
    12181 cgcttcatcg tctgcatgcc cagccgcccg cgcaaggatg gccgtcacca ggatgtggcg
    12241 catcccatca atgcgccgac gcgcgatcgt ttcaaccgcc acatcctcga tgcctacgaa
    12301 gatgagctcg gccgcttcga gcggggcgaa gggccctccc accatccgga cgatgaccag
    12361 ggaccacagg ggatctaccc ggcatgaagt ccgtcctctt ctggatcacg gcggccatca
    12421 cgatggtcgt ctcggtggcc gtttccctgg tctatttcaa cctggcgatg aacctgatcc
    12481 cggcggcctt cctgtcgcag ttcaaccggg ccgccgccca gaccgcattc atcttctggg
    12541 ggatcgggct gggcctggcg gtggcggttc tggagggcct cctcttctgg atcctcccca
    12601 gggtccttcc gcggcgctcg gcggcctgag gcgtattgca cgatggtccg gggcatttcg
    12661 cccgccggat cagcacccac cccgtgccat ggccggccag cccgggagtg ccccggcccg
    12721 ctgaattctg cgcgcccata atccgtttga cccgtaagga ctaggttgct agattagagc
    12781 atcactttca gtccgaatga acaggcattt gttgacgcag cctggctgga gtggtcgaat
    12841 tcttgcgttt gcggtccggg aggatgtgat gccgccggtg cggatgaccg aaacgctcca
    12901 ccccaccatt cgagggagtt tcaagcaatg attggacggc gatcgcgttg gttgctcgtg
    12961 gtccttggac tgaccctggc actcaccccc ctcgttgacg gcgtcctcgc ggttcgatcg
    13021 gtcgaggcgg ccggcacggc caattccacc accaggtctt ccaccaagtc gaccaccaag
    13081 aaagcgacgc cggccaagtc gacctcctcg aagaagtcga ccacaagcac gaagaagacc
    13141 ggcggcaaat ccaccgccag gacatcgacg tcggtcaaga agtcctccgg caagagcgcc
    13201 gggggaaaga aagccacgac caagcgcacc gtctccaggc ggaaccgtaa atcggcccgg
    13261 cggccggctc ccgtcgtcgc cttcgatccc gcccgtccgg ccacgctccg tgcggagtcg
    13321 gccgtcgttc tcgatcggcg caccggtcac gtgctgtggg gcaagaacgc ggatgaaaca
    13381 cggccggtgg ccagcctcac caagctcatg accgcgctgg tcgtctccga atccccgctg
    13441 gccatggacg actcgatcac ggtcaccatg gacgacgtca ccggcgccgg ccggagccac
    13501 gtgcgtgccg gcaaccgcgt gtcgatcggg gatctctacc actgctcgtt gatctcctcg
    13561 gacaacgccg ccacgcgcgc gctggcccgc gccaccggaa tgagctcgac ggcgttcgtg
    13621 gcccggatga accagcgcgc ccgcgagctc ggcatggaac actcccacta tgtcgaaacc
    13681 accggcctcg acgccggcaa cgtctcgacg gcgcgggacc aggcgcgc
//
LOCUS       DASVOD010000467         5339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_448878, whole genome shotgun sequence.
ACCESSION   DASVOD010000467 DASVOD010000000
VERSION     DASVOD010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448878"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..529
                     /locus_tag="VF720_10590"
     CDS             98..529
                     /locus_tag="VF720_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX7879849.1"
                     /translation="MQPFLGKFETPLYTALRIVAGFMFSLHGAQKLFGFLGNKAPAGN
                     MLMLSAGVIEFFGGILIAIGLFTSFVAFLASGQMAVAYFKSHAPSGFWPTANHGELAA
                     LYCFLWLFVSAHGAGPYSLDSVLFGAPRKRAGGPSSVVPGR"
     gene            647..2992
                     /locus_tag="VF720_10595"
     CDS             647..2992
                     /locus_tag="VF720_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879850.1"
                     /translation="MPGCRPAPFLPPATRLVRLAVILAAWFAAVPVAAAPYDGQPKIL
                     LHLGTVTSKSACIAGERSDCGTAVTAGEVGGAAGPFYYAYVLAAKGNLPDLAGVQFGI
                     RYENGCVNGRNDNQQVDVLAWTLCGSLEFTTPGFYSWPGPNSGTTITWDPGPRCQVGE
                     TAVAGYFYVACYGAADTMRVIPRPVDGLAAVAQCNSNVTNLPVASLGSAVFSPGGSVA
                     GCNPCNGPCSPPPTSLSCGPGVDVTPPAPVTLQLQQRTDTAVTVRWAAPGDDGTTGGP
                     ATSYDLRWFSDYPYDFAIGTPVPGVPAPAAPGTLQSATTTGLSPGGTYWFVLRSADEM
                     PNWSVLSNRLSVTTLTMPPDLTVPAAIADLAQVDATLTTLRVGWTATGDDGAVGTASS
                     HEMRRAASPITTDAEFVAATLVTGLPAPAAAGTHQEVTLTGLAPGTTCYLAIRARDEA
                     ANESPLSNSPACTTSARNESIPPAAVTDLKALAATPGAVRLGWTAPGDDGSTGTATTY
                     DIRYATSPITAANFASAVPATGEPVPLPAGTPQELTITTLSAGVVYYFAMKSVDDQEN
                     LSDLSNVVSRRATDNSGARILLHSRIYTTKNVCAIGQLADCGTAAVNNQPLYPPARFT
                     YLLAGKFGDVGALQCGIQYDQGAPDGDANARGVDVWGWTLCADLESPSTAPIWPKPGS
                     GNLISWNAATKCQVGGTAVAGYFYMGAYTSDRFQVTPRPSDGLARVYDCAGASQDLTA
                     AALGSVGYGTRQGYNPCSASGPPSLEVRPTTWSQIKTLIRN"
     gene            3125..4408
                     /locus_tag="VF720_10600"
     CDS             3125..4408
                     /locus_tag="VF720_10600"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEX7879851.1"
                     /translation="MSTPPDSARLDAAIEELDFLNDVATKVARAESPEAAEQAIITTC
                     VERVGAEQGAIWKMSMAETSPLATVARVFEGGIPGLPLRLNLQILDWIQRQQAPLLTN
                     DVAGDPRLKMGSAGSPAGLRSLLAVPLFYKKQIIGLLAVLNAKSAEGFDEADQRLLSI
                     VGMQCAQIQENARLAKEESKLKELERELDAARAIQHSLVPAVMPQIPGWELAAAYEPA
                     RQVGGDLYHVDRTADALHVAVADVSGKGMPACLYMVNVVTNMRALAAQKLAPAELMSR
                     LNALLLSTMTDGKFVTLFWASVDPVTGAVSYCNGGHNPALVLRTNGEVEWHNDGGTLV
                     GLLPDTPFEGGSLTLGPGDRFILYSDGVTEAVSPDADFFGDDRLVDATRSVSATGPAT
                     PAALVEAILSAVKAHEAGEPAADDKTLVVVSRQAG"
     gene            4422..>5339
                     /gene="moaA"
                     /locus_tag="VF720_10605"
     CDS             4422..>5339
                     /gene="moaA"
                     /locus_tag="VF720_10605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX7879852.1"
                     /translation="MSNGLIDRFGRTHDNLRLSVTDRCNLRCFYCMPEDNPDYMPRAE
                     LLSFEEIVRIVRVAAGLGVTKLRLTGGEPLVRRGIADLVGMLARIDGIREVAMTTNGL
                     LLANLAEPLRAAGLSRLTVHLDSLDRERFRQMVRRDALDQVLAGIEAAGQAGFDAIKI
                     NAVAIKGLTEPDIVPLARFGREHGHEIRFIEFMPLDADHGWRRDQVLLADDILAALTA
                     GIGPLEPVPDADPRAPAQEYRYTDGIGRVGLIASISKPFCGNCNRIRLTADGKLRNCL
                     FALGEADVRALLRGGADDDAITALLRGNVA"
BASE COUNT          839 a         1934 c         1720 g          846 t
ORIGIN      
        1 gcctcttcct ggtggcgcgg aagggctgac ccaccggtcg cgtggcacga ccggtgctag
       61 cctcgaattg tggtttgaca tcaattcgga ggtcctcatg caaccgtttc tcggaaagtt
      121 cgagacgccg ctttacacgg ccctgcgcat cgtggccggc ttcatgttct ccctgcacgg
      181 cgcgcagaag ctgttcggat tcctcggcaa caaggctccg gcgggcaaca tgctgatgct
      241 gtcggccggc gtcatcgagt tcttcggcgg gatcctcatc gcaatcggcc ttttcaccag
      301 tttcgtggcc ttcctggcca gcggccagat ggcggtagcc tacttcaagt cacacgcccc
      361 ttcgggcttc tggcccaccg ccaatcacgg cgagctggcc gcgctctatt gtttcctctg
      421 gctcttcgtg tcggcgcacg gcgccgggcc ctacagcctc gattccgtac tgttcggggc
      481 accgagaaag cgggccggcg gcccgtcgtc ggtggtcccc gggcgctagt tccggattgc
      541 cgggccgcgc ccggttccct acactattgg tcagttcccg ggcggaactg agcctccgct
      601 ccccttgcga cgcgttcaac gaaacctgcc acagggagtg cccaccatgc cgggttgccg
      661 ccccgccccg ttcctcccgc ctgccacccg acttgtccgc ctggccgtga tccttgccgc
      721 atggttcgcg gcagtaccgg tcgcggccgc cccgtacgat ggccagccca agatcctgct
      781 ccacctcggt acggtcacat cgaagtcggc ctgcatcgcg ggagaacgat ccgactgcgg
      841 caccgcggtc acggcgggtg aggtgggcgg cgccgcgggg cccttctact atgcgtacgt
      901 cctggccgcg aagggaaatc tccccgacct ggccggcgtg cagttcggca tccgctacga
      961 gaacggctgc gtgaacggcc ggaacgacaa ccagcaggtc gacgtcctcg cgtggacact
     1021 gtgcggctcg ctggaattca cgacgcccgg tttctacagc tggccgggac cgaacagcgg
     1081 gaccacgatc acgtgggacc cgggtcccag atgccaggtg ggcgagacgg ccgtggccgg
     1141 ctacttctac gtggcgtgtt acggagcggc cgatacgatg cgggtcattc cccggcccgt
     1201 ggacggtctc gcggccgtgg cgcaatgcaa ctcgaacgtc accaatctcc cggtcgcctc
     1261 gctcggcagc gccgtctttt cgcccggcgg cagcgtggcc ggctgcaacc cgtgcaatgg
     1321 accgtgcagc ccgcccccca cctcactgag ctgcggcccc ggcgtggacg tgacgccgcc
     1381 ggcgccggtc acgctccagt tgcagcagcg gaccgacacg gccgtcaccg tccggtgggc
     1441 ggcacccggc gatgacggca cgaccggcgg accggccacc agctacgacc tgcgctggtt
     1501 ctcggactat ccctacgatt tcgcgatcgg cacgccggtg ccgggcgttc cggctcctgc
     1561 cgcaccgggc accctgcaga gcgccaccac caccgggctc tcccccggcg gaacgtactg
     1621 gttcgtcctg cggagcgccg acgagatgcc gaactggtcg gtcctctcca accggctgtc
     1681 ggtcacgacg ctgaccatgc ctcccgatct cacggttccg gcagccatcg ccgacctggc
     1741 acaggtcgat gccacgctga ccacgctgcg ggtgggatgg accgcaaccg gcgatgacgg
     1801 tgcggtcgga actgcatcca gccacgagat gcgtcgggcg gcaagcccga tcacgacgga
     1861 cgccgagttc gtggccgcca cgctcgtgac cgggctcccc gcacccgccg cggcgggaac
     1921 gcaccaggaa gtgaccctga ccggccttgc gccggggacg acctgttacc tggccatccg
     1981 cgcacgtgac gaggcggcga acgaatcgcc gctgtcgaat tcgccggcct gcacgacgtc
     2041 ggcccgcaac gaatcgatac cccccgcggc cgtcaccgac ctgaaagcgc tcgcggcaac
     2101 gcccggcgcg gtgcggctgg gatggaccgc cccgggggat gacggatcga ccggcacggc
     2161 caccacgtac gacatccgct acgccacgtc gccgatcacc gcggccaatt tcgcgtcggc
     2221 ggttccggcc accggtgagc ccgtgccgct gccggccgga acgccacagg aattgacgat
     2281 caccaccctc tcggccggcg tcgtgtatta cttcgccatg aaatcggtgg acgatcagga
     2341 gaacctgtcg gacctgtcga acgtggtcag ccggcgggca accgacaact cgggcgccag
     2401 gatcctgctt cactcacgca tctacacgac gaagaatgtc tgcgcgatag gccagctggc
     2461 cgactgcgga accgccgccg tcaacaacca gccactctac ccccccgccc ggtttaccta
     2521 cctgctggcg ggcaagttcg gcgatgtcgg cgcgcttcaa tgcggcatcc agtacgacca
     2581 gggggccccc gacggagacg ccaatgcccg cggcgtcgac gtgtggggct ggactctctg
     2641 tgccgacctg gagagcccct cgacggcccc gatctggccg aaacccggct ccggcaacct
     2701 gatctcgtgg aacgcggcca cgaagtgcca ggtcggcggc accgcggtgg ccggctactt
     2761 ctacatgggc gcctacacga gcgatcgctt ccaggtgacg ccccgtcctt ccgacggcct
     2821 ggcccgcgta tacgactgcg ccggcgcgtc ccaggacctc acggcggcgg cgctggggtc
     2881 ggtggggtac ggcacgcgcc agggatacaa tccatgttcc gccagcggtc ccccctcgtt
     2941 ggaagtccgt cccacgacct ggagccagat caagacgctg atccgaaact gattcgggat
     3001 caggttgcga cttctctcca gcaacacggt acgcgttaca ggatcccagc ggattggcat
     3061 ttgcgccggc accggccggg gcgtaccatc atcggcttca cccctttccc ccggagatct
     3121 gcccgtgtcc acgccccccg attccgcccg cctcgatgcc gcgatcgagg aactcgactt
     3181 cctgaacgac gtggccacga aggtggcacg ggcggagagc ccggaggccg ccgaacaggc
     3241 catcatcacc acctgtgtcg agcgcgtggg cgccgagcag ggggccatct ggaagatgtc
     3301 gatggccgag acctcccctc tggccacggt ggcccgcgtc ttcgagggtg gcatccccgg
     3361 gctgccgctc cggctcaatc tccagatcct cgactggatc cagcgccagc aggcgccgct
     3421 cctcaccaac gacgtggcgg gcgatccgcg cctcaagatg ggctcggcgg gaagcccggc
     3481 cggcctccgc tcgctgctgg cggtgccgct cttctacaag aagcagatca tcggcctgtt
     3541 ggccgtgctc aatgccaagt cggccgaggg gttcgacgag gcggaccagc gtctcctctc
     3601 gatcgtcggg atgcagtgcg cccagatcca ggaaaacgcc cggctggcca aggaagagag
     3661 caagctcaag gaactcgagc gcgagctcga cgcggcacgc gccatccagc attcgctcgt
     3721 gccggccgtc atgccgcaga ttcccgggtg ggagctggcc gcggcgtacg agccggcccg
     3781 ccaggtgggt ggtgacctgt atcacgtgga ccggacggcc gacgcgctcc acgtggccgt
     3841 ggccgacgtc tccggcaagg ggatgcctgc ctgcctctac atggtgaacg tcgtcaccaa
     3901 catgcgggca ctcgccgcac agaagctggc gccggccgaa ctgatgtcgc gactcaacgc
     3961 gctcctcctc tcgacgatga ccgacggcaa gttcgtcacg cttttctggg cctcggtcga
     4021 tcccgtcacg ggcgccgtgt cgtactgcaa cggcggccac aacccggcgc tggtgctccg
     4081 caccaacggc gaggtggagt ggcacaacga cggcggcacg ctggtgggcc tccttcccga
     4141 caccccgttc gagggaggct cgctcacgct cggaccgggc gatcgcttca tcctctattc
     4201 ggacggcgtc accgaggcag tgagccccga cgcggatttc ttcggcgatg accgcctcgt
     4261 cgacgccaca cgcagcgttt ccgccaccgg tccggccacg ccggccgcac tggtcgaggc
     4321 catcctgtcg gccgtcaagg cgcacgaggc cggggaaccg gccgccgacg acaagacgct
     4381 ggtggtcgtg tcgcggcagg cgggttgatc ggagttttac cgtgagcaac ggcctcatcg
     4441 atcgcttcgg ccgcacgcac gacaacctgc gcctgtcggt gacggaccgg tgcaacctcc
     4501 gctgcttcta ctgcatgccg gaggacaatc cggactacat gccgcgggcg gagctccttt
     4561 cgttcgagga gatcgtgagg atcgtccgcg tggctgccgg actcggcgtc acgaaattgc
     4621 gtttgaccgg gggcgaaccg ctggtccgtc gtggcatcgc cgacctcgtg ggaatgctgg
     4681 cccggatcga cggcatccgc gaagtggcga tgaccaccaa cggcctcctg ctggccaacc
     4741 tcgccgagcc gctccgggcg gccggacttt cgcgcctgac cgtccacctc gattcgctcg
     4801 atcgcgagcg cttccgtcag atggtgcggc gcgatgcgct cgaccaggtg ctggccggaa
     4861 tcgaagcggc gggacaggcc ggcttcgacg ccatcaagat caacgccgtg gccatcaagg
     4921 ggctcaccga gcccgacatc gtgccgctgg cacggttcgg ccgcgagcac ggccacgaga
     4981 tccgcttcat cgagttcatg ccgctcgacg ccgaccatgg ctggcggcgc gaccaggtcc
     5041 tcctggccga cgacatcctg gcggcgctca ccgccggcat cggaccgctc gaaccggtgc
     5101 ccgacgccga ccctcgcgcc cccgcgcagg aatatcgcta caccgacggc atcggccgag
     5161 tcggcctcat cgcctcgatc agcaagccct tctgcggcaa ctgcaaccgg atccgcctga
     5221 ccgccgacgg caagctgcgc aactgcctct tcgcgctcgg tgaagccgac gtgcgggccc
     5281 tcctgcgcgg cggagcggac gacgacgcca tcacggccct cctccggggc aacgtggcc
//
LOCUS       DASVOD010000468         4451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_84014, whole genome shotgun sequence.
ACCESSION   DASVOD010000468 DASVOD010000000
VERSION     DASVOD010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84014"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VF720_10610"
     CDS             complement(<1..588)
                     /locus_tag="VF720_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX7879853.1"
                     /translation="MKLPGMRLRTIRPAFALGLLVSLAAVACGDSGKETDMSSGPALD
                     PALLLPRVEARLAAIEPTMIEVRHDIHRHPELSGQESRTAGLVADRLREAGFDVRTGV
                     GGHGVVARLTAPRPGRSVGVRADMDAVRSNAHDPADFRSETPGVRHICGHDIHTTTAL
                     AAALALAAVQTDLAGSVVFYFQPAEENVTGAWAMLH"
     gene            complement(603..1340)
                     /locus_tag="VF720_10615"
     CDS             complement(603..1340)
                     /locus_tag="VF720_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879854.1"
                     /translation="MTLPWLPPDHAANGHLVDDTLLYVHLVMGVVALGWAVYYLVVVA
                     RARRASDAGAATNRTPDRLPLRLAWLLVGGIAIAEAALFLGKELPAWSVLREVPPDDD
                     TVVHLRVVAEQFAWNVHYPGPDGRYGRTDPARVSQLNPVGIDTTDEAARDDVIRLNQI
                     HLPIGRPALIELSSKDVIHAFHLPVMRVKQDVIPGQRARAWFTPVVADTTDIACAQLC
                     GLGHYRMKGELIVESEADFQAWLSGKE"
     gene            complement(1337..1651)
                     /locus_tag="VF720_10620"
     CDS             complement(1337..1651)
                     /locus_tag="VF720_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879855.1"
                     /translation="MTSNRSTRSTRHPGRFRYTLLPALATSATLAIGTAEACPVCFGE
                     ANTGASHGLTAAIGVLGGTALFVVGAITVVVLRIRARAAAHAFLSANPASLQNPPDDS
                     PR"
     gene            complement(1648..2238)
                     /locus_tag="VF720_10625"
     CDS             complement(1648..2238)
                     /locus_tag="VF720_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX7879856.1"
                     /translation="MNRRELPWATEVRPDTGLVSSRLGLWLFMASETMLLAGLVSALF
                     FLRLASDDWPREALEHGLPLVNSIVMASSALMLFLARRSLGVGSREFMLQALLGVALG
                     FLVLFIRSAEWSILLGMGRTPAVHNLFGIYFVLTGVHAVLLAGNLAVLAWILSAGRTL
                     AATHPARFRERAQGAVTHWHFLTLVWFVIHGLFFQS"
     gene            complement(2235..3803)
                     /locus_tag="VF720_10630"
     CDS             complement(2235..3803)
                     /locus_tag="VF720_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX7879857.1"
                     /translation="MTDAATVLERAPAPSDHRTIALRFGLTSLFFLLVGFLLILLIRW
                     QLAWPGRPLPLIGGWFPASMAPGGIILPEFYNALGAMHGTVMIFLGVVPLGVGTFGNL
                     LVPGMIGAPEMAFPRLTAASWWTFFLAGIAGLAGFVVPGGPAASGWTSYPPLALLATL
                     GQDLWLVCVLLLGISSMLGAINMIVTVVQLRRPGLGWRELPFFVWAQLITALLLLLAF
                     PPLQSAAVLQLMDRLGGTSFFSPLGLIVSGVSRAGAGGGSPILWQHLFWFLAHPEVYV
                     LLLPALGIVAHILVESTGRPLYGERSMVGALHFLGVMSMLVWAHHMFLTGMGPVMSAF
                     FQATTLIISIPSVIILTCLFLTLRGGFRGGTSTLFALGFLPMFGIGGLTGLPLGLAVG
                     DIHLHDTYYVIGHFHYIVAPGVLIALFAGIYHWWPLIAGRPLDERLGRVHFWGTMIGM
                     NGVFLPMFAMGLAGVSRRLWDGGAGYEHAASTIRFNVFMTHAAWLVAVAQAPFLWNVL
                     ANLPRRGATRVGRT"
     gene            complement(3825..>4451)
                     /locus_tag="VF720_10635"
     CDS             complement(3825..>4451)
                     /locus_tag="VF720_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879858.1"
                     /translation="STRATPAILLLAGWILTPALATAASWSEQGDAGDLPATAQITAG
                     AGGLTQITGSLPSDADIDMYCFRVTDRASFFAFRICAGIAEPDLWLFDQNGAGIELND
                     GCMFSQTNLSAGFVPTNGLYYLAVSLSDRDARNAGGQIIWNSPSQSAPRAPDGPGAPG
                     PLVSWGGSAFPLGPVTYSIQLNGATFCDIPVPAEPASWGTVKSRFVDP"
BASE COUNT          804 a         1390 c         1698 g          559 t
ORIGIN      
        1 gtgcagcatg gcccacgcgc cggtcacgtt ctcttcggcc ggctggaagt agaagaccac
       61 cgagccggcc aggtcggtct gcaccgcagc cagtgcgagc gcggcggcca gcgcggtcgt
      121 cgtgtggatg tcgtgcccgc agatgtggcg cacgcccggc gtttcggagc ggaagtcggc
      181 ggggtcgtgc gcgttggacc ggacggcatc catgtcggcg cgaacgccca ccgacctgcc
      241 cgggcgcggg gcggtgagcc gcgccaccac gccatggccg ccgacaccgg tgcgcacgtc
      301 gaagccggct tcacggagcc ggtcggccac gaggccggcc gtgcgggatt cctgtcccga
      361 gagctcagga tgccggtgga tatcgtgccg cacctcgatc atggtcggct cgatggcggc
      421 gaggcgtgct tccacgcggg ggagcagcag ggcgggatcg agtgcggggc cactggacat
      481 gtccgtttcc tttccggagt cgccgcaggc gacggcggcc aggctcacca ggagacccag
      541 cgcgaaggcc ggacggatcg tgcgaaggcg catccccgga agcttcatgc cgaaaggatg
      601 actcattcct tgcccgagag ccatgcctgg aaatcggcct cgctctcgac gatcagctcc
      661 cccttcatcc ggtagtgccc caggccacag agctgcgcgc aggcaatgtc ggtcgtgtcg
      721 gccacgacgg gcgtgaacca ggcccgggcg cgctggccgg ggatcacgtc ctgcttgacc
      781 cgcatcaccg gcaggtggaa cgcgtgaatg acatccttgc tggagagttc gatcagggcc
      841 gggcggccga tcggcaggtg gatctggttg agccggatga cgtcgtcgcg ggcggcctca
      901 tcggtcgtgt cgatgccgac cgggttcagc tgcgaaacgc gcgcgggatc ggtgcggccg
      961 tagcgtccat ccggcccggg gtagtgcacg ttccatgcga actgctcggc gacgacgcgc
     1021 aggtgcacga ccgtgtcgtc gtcgggtgga acctcgcgga gaacggacca ggcagggagt
     1081 tccttgccga ggaagagcgc cgcctcggcg attgcgatgc cgccgaccag gagccaggcg
     1141 aggcgcaggg gaaggcgatc gggggtgcga ttggttgcgg cgccggcatc ggaggcgcgt
     1201 cgggcgcggg ccacgaccac gaggtagtag acggcccagc cgagtgccac gacgcccatg
     1261 acgaggtgca cgtagaggag cgtgtcgtcc acgagatggc cgttcgcggc gtggtcgggc
     1321 gggagccagg gaagcgtcat cggggcgagt cgtccggggg gttttggagg ctggccgggt
     1381 ttgcggagag gaacgcgtgg gcggcggccc gggcgcggat gcgcagcacc acgacggtga
     1441 tcgcgccgac cacgaagagc gcggtgcctc cgaggacacc gatggcggcc gtgaggccgt
     1501 gggaggcccc ggtgttggcc tcgccgaagc agacggggca ggcttcggcg gtgccgatcg
     1561 ccagggtcgc cgatgtcgca agagccggga ggagggtgta tcggaatcgg cccgggtgtc
     1621 gcgtggagcg cgtcgatcga ttggacgtca tgactggaag aaaagcccgt ggatgacgaa
     1681 ccacaccagc gtcaggaaat gccagtgcgt gacggcgccc tgcgcgcgtt cgcggaaccg
     1741 ggcggggtgg gtggcggcga gtgtgcggcc ggccgacagg atccacgcaa ggacggcgag
     1801 attgcccgcc agcaacaccg cgtgcacgcc ggtcaggaca aagtagatgc cgaacaggtt
     1861 gtggacggcg ggggtgcgcc ccatgccgag caggatcgac cactcggccg agcggatgaa
     1921 cagcacgagg aagccgaggg ccacgcccag cagggcctgc agcatgaatt cgcgcgagcc
     1981 gacgccgagc gagcgtcggg ccaggaagag catcagcgcc gaggaggcca tgacgatgga
     2041 gttgaccagg ggcaggccat gctccagtgc ttcgcgcggc cagtcgtccg acgccaggcg
     2101 aaggaagaag agcgccgaga cgaggccggc cagcagcatc gtttccgagg ccatgaagag
     2161 ccagaggccg agccggctcg agacgaggcc cgtgtcgggg cgcacctcgg tggcccacgg
     2221 gagttcgcga cggttcacgt gcgccccacc ctggtggcgc cgcgtcgcgg caggttggcc
     2281 agcacgttcc acaggaaagg tgcctgcgcc accgccacca gccacgccgc gtgcgtcatg
     2341 aagacgttga agcggatcgt cgacgccgcg tgctcgtagc cggcgccgcc atcccacagc
     2401 cttcggctga cgccggccag ccccatggcg aacatcggga ggaagacgcc gttcatcccg
     2461 atcatcgttc cccagaaatg cactctgccg aggcgctcgt cgagcggacg gccggcgatg
     2521 agcggccacc agtggtagat gccggcgaag agggcgatga gcacgccggg cgccacgatg
     2581 tagtggaagt ggccgatcac gtaatacgtg tcgtgaaggt ggatgtctcc caccgcaagg
     2641 ccgagcggga ggcccgtcag gccgccgatg ccgaacatcg ggaggaaacc gagggcaaag
     2701 agcgtggagg tgccgccacg gaatccgccg cgcagcgtga ggaagaggca ggtcaggatg
     2761 atcacgctcg ggatcgagat gatgagcgtg gtggcctgga agaaggcgct catcacgggg
     2821 cccatcccgg tcaggaacat gtgatgcgcc cacacgagca tcgacatgac gccgaggaaa
     2881 tgaagcgcgc ccaccatcga ccgctcgccg tacagcggcc gcccggtgct ctccacgagg
     2941 atgtgggcca cgatgccgag cgccggcaac aggagcacat atacctcggg gtgcgccagg
     3001 aaccagaaga ggtgctgcca gaggatcggg ctcccccccc cggcccccgc gcgggacacg
     3061 cccgacacga tgaggccgag cggcgagaag aacgacgtgc cgcccagccg gtccatgagc
     3121 tgcagcacgg ccgccgactg gagcggcggg aaggccagga gcaacagcag ggcggtgatg
     3181 agctgggccc agacgaagaa cggcagctcc cgccagccga gccccggtcg acgcagctgc
     3241 acgacggtga cgatcatgtt gatggcgccg agcatcgacg aaatgccgag cagcagcacg
     3301 cagacgagcc agaggtcctg gccgagcgtg gccagcagcg ccagcggcgg gtagctggtc
     3361 cagccggaag cggcgggccc gcccggcacc acgaagccgg ccaggccggc gatccccgcc
     3421 aggaaaaaag tccaccacga ggcggccgtg aggcgcggga aggccatttc aggggcgccg
     3481 atcatccccg gcacgaggag gttgccgaag gtgcccacgc cgagcggcac cacgccgagg
     3541 aagatcatga ccgtgccgtg catcgccccg agcgcgttgt agaactcggg gaggatgatg
     3601 ccgccggggg ccatcgacgc cgggaaccac cccccgatga gggggagcgg ccggccgggc
     3661 caggccagct gccagcggat gaggaggatg agcaggaatc cgaccagcag gaagaacagg
     3721 ctggtcaggc cgaagcgcag ggcgatggtg cggtggtcgg aaggtgcggg cgctcgttcg
     3781 aggacggtcg cggcgtcggt cacgtcgggg tcagcgactc cgggtcatgg atcgacgaat
     3841 cgggacttca cggttcccca cgaggcgggt tcggccggca ccgggatgtc gcagaacgtg
     3901 gcgccattga gctggatgga gtaggtgacc gggcccaggg gaaaggccga gcccccccag
     3961 gaaacgaggg ggccgggagc gccgggtccg tccggggcgc gcggcgcgct ctgggacggg
     4021 gaattccaga tgatctgccc ccccgcgttc cgggcatcgc ggtcgctcag gctgacggcc
     4081 aggtagtaga gcccgttggt ggggacgaac cccgccgaga gattagtctg tgaaaacatg
     4141 caaccgtcgt tgagctcgat gccggcgccg ttctggtcga agagccagag atcgggctcc
     4201 gcgatgccgg cgcagatccg gaaggcgaag aacgacgccc ggtcggtgac gcggaagcag
     4261 tacatgtcga tgtccgcgtc cgaggggagc gaaccggtga tctgcgtcaa accacccgct
     4321 ccggccgtga tctgggcggt ggcggggagg tctccggcgt ctccctgctc actccaggag
     4381 gcggccgtgg ccaatgcggg tgtcagtatc catccggcca gcaacaggat ggccggggtg
     4441 gcgcgggtcg a
//
LOCUS       DASVOD010000469         5322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_239809, whole genome shotgun sequence.
ACCESSION   DASVOD010000469 DASVOD010000000
VERSION     DASVOD010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239809"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5322)
                     /locus_tag="VF720_10640"
     CDS             complement(<1..>5322)
                     /locus_tag="VF720_10640"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX7879859.1"
                     /translation="TTVAAGATLRGNQVTIGGALSNQGSVRATGALALTGALTTSATS
                     SIVIEPSAGTAGITVTNGFTNLGTIDLSASGGAPVSTLTVTNGTLVNESGGTIRASLA
                     SGGARSILAQLDNRGLLEVGVSTTLDKAGADHLNSGTVDLVAGGSGIFTLLQSGTTPT
                     FISAGNVALGSGRTLSVSGGLFQDAGGSISGTGTIALSTATATFDNGFSNAVTGITLA
                     GTCTINGGPFTVAVGKSFTLPANATLNAPLINQGTTTFVSTNISLTGAVSNTAGGILS
                     LNTGAVVTVASGFTNQGSLQMSAAGLSNSTFNVGSGTLINDVGGEIRSIGSPGFATRI
                     LGCQLDNRGLVSLANGNMTLSKAAATHLNSGTIDITGGNFSLTQSSTSPSFTTSGTIS
                     VAASRSFIVTGGVLHKAGGTISGAGTLSLNNVNATFDNGLSTGDIALSLGGNNTIGGG
                     AFTNPSGRTVTVPGTLVLNSSLDNAGTFKLSGTLLAINGALTTAAGSLLSVERQNNTG
                     DGRMEVANGFTNLGTIELSSINIPANSVLAVANGALINQTGGMIRSLPGTGGTRKLEL
                     LLDNRGTVSVQHPLTLRRTTGGVEDNTGTIDIVAGPFAIDLSGTSFSNGGIITIGPGQ
                     LLTSVGSAPNFTNQAGGVIQGSGTFEVGPMHFINNGTVRPGTSPGILTFNDIFHQGPS
                     GDLDIEIGGTTAGTGYDRLVATQFANMSGDINVSLIDGFVPSYDDSFDIVTFEERTGT
                     PNITGMLLPNGFYLEPRFSDRKISLHVINDPNRPDTEILSGPGAATGATTAHFSWTGT
                     DIQTPTAALTYSWSLDGAPFIPDAPDTSVTLTGLVPGSHTFLVRAHDGDGLSDPTPAE
                     QTWLIDVVPPLVILGGGPAENALVNTPDVTFTFHATDNTTPAAALEYSWQIDAGSPTP
                     FAADTTANLTALAEGAHTFVVRARDAYGNLGLATRTFTVDLTPPETAITGGPGDGGYT
                     NMLSVSFAWQGTDANTGLVFATKLDDDPESAFSPATSGEFPFVGTGPHTFRVRARDAA
                     GNEDASPAARSFIVDQEAPTAAIVAGVAEGGVLDTDMAAFAWTGMDGQVPREELLFST
                     SLDAGAFGTPSADSTTSLTGLADGPHTFRVRSHDPAGNASAPEVRNFIVNNLPPVVTI
                     LSGPADGSYDNDTDAAFTWDGHDAVSPDNTLLFQWRLDGGAYSPFDAGTMASLNALQD
                     GTHTFEVVGRDEVGKTGSASRTWTVDLVPPTMTTCTLRLLDGSVLQATATGADDRFLA
                     GFHLQVATDFDFTAILQETDVSPAGLATFPGMPGTSYFVRARALDAAGNASPYSPPSN
                     GVTLAFLPDLVVGPIVVPPSANSGQTLQVSWEVHNDGEGPTQVPVWVDDLYLSPTPSW
                     NSASSVLLGRFENTSYLAPGEAYTTTRQVTLPRGASGLYYVHIKTDNNDQQAESSAAN
                     NTAGSTSFTIFLSDHADLRATSVVVPPTAFSGEAIDVQWSVRNQGSGRTDVDQWWDLI
                     VLSPDSTLNVSLAPGQLRIFDQPLTRFRHTGALEPGESYSRSESITLPQGIDGDYYII
                     VFPDIGITASGQFVPEDGEVFENVLFTNNWNNDGLEVTLTPPADLVVDAVSAPPSVSA
                     GVPFPVSWVVRNAGLNPTFETWWSDRLWLSTDGVLQTGTDTPLADVVRSTWLDRDSSY
                     VASATVTLPNGTSGTWQLLVQADRSASVLELDEDNNVGVAPAATTVSVPPWADLMVEE
                     VTAPPAGSSGGSVDVTWKVRNIGAAPANGTWYDQVIISTSPVSATGT"
BASE COUNT          830 a         1627 c         1979 g          886 t
ORIGIN      
        1 ccgtgccggt ggccgagacg ggcgacgtgg agatgatcac ctggtcgtac caggtgccat
       61 tggcgggggc ggcgccgatg ttgcgtacct tccaggtgac gtcgaccgag cctcccgaac
      121 tgccggcggg cggagcggtc acttcctcca ccatcaggtc ggcccacggc ggcacgctca
      181 ccgtcgtggc cgcgggagcc acgcccacgt tgttgtcctc gtcgagctcg agcacggagg
      241 cggaacggtc ggcctgcacc agcaactgcc aggtgccgga cgtgccgttg ggaagcgtga
      301 cggtggctga tgccacgtag ctcgaatcgc gatcgagcca ggtggagcga accacgtcgg
      361 ccagcggcgt atcggtaccg gtctgcagta cgccgtcggt cgagagccag agccggtcgg
      421 accaccaggt ctcgaacgtg ggattgagcc cggcgtttcg gaccacccac gagacgggga
      481 acggcacgcc ggcactcacg gagggaggcg ccgagaccgc gtcgaccacc aggtcggccg
      541 gcggggtgag cgtgacctcg aggccgtcgt tgttccagtt gttggtgaag aggacgttct
      601 cgaacacctc cccgtcttcg ggcacgaact ggccggaggc ggtgatgccg atgtcgggga
      661 agacgatgat gtagtagtcg ccgtcgatcc cctgcggcag cgtgatcgat tcgctccgcg
      721 aatagctttc gccgggctcg agggcgccgg tgtgccggaa tcgcgtcagc ggctggtcga
      781 agatgcggag ctgccccggc gccagcgaca cgttgagcgt ggagtcgggg gagaggacga
      841 tgaggtccca ccactggtcc acgtcggtcc ggccgctccc ctggttccgg acgctccatt
      901 gcacgtcgat cgcctcgccg gagaaggcgg tgggcggcac caccaccgag gtggcccgga
      961 ggtcggcgtg atccgagagg aagatggtga aggaggtcga gcccgcggtg ttgttggccg
     1021 cggagctctc ggcctgctgg tcgttgttgt cggtcttgat gtgcacgtag tacaggccgc
     1081 tggccccgcg cggcagcgtg acctggcgcg tggtggtgta cgcctcgccg ggcgccaggt
     1141 acgaggtgtt ctcgaagcgc ccgaggagca ccgacgacgc ggaattccac gacggcgtgg
     1201 gagagagata gaggtcatcc acccagacgg gcacctgggt ggggccctcg ccgtcattgt
     1261 gcacctccca ggagacctgg agcgtctggc cggagttggc gcttggcggc accacgatcg
     1321 ggccgaccac cagatcgggc aggaacgcca gcgtgacgcc gttggacggt ggactgtagg
     1381 ggctcgcgtt accggcggcg tcgagcgcgc gcgcgcgcac gaagtagctc gtgcccggca
     1441 tacccgggaa ggtggccaag ccggccggcg agacgtccgt ttcctggagg atggcggtga
     1501 aatcgaaatc ggtggccacc tggagatgga atccggccag gaaacggtcg tcggcaccgg
     1561 tggcggtggc ctgcagcacg ctgccgtcga ggaggcgcaa cgtgcacgtg gtcatcgtgg
     1621 gcggaacgag gtccaccgtc caggtgcggc tggccgagcc ggtcttcccc acctcgtcgc
     1681 ggccgaccac ctcgaaggta tgggtgccgt cctggagcgc gttgagcgat gccatggtgc
     1741 cggcatcgaa cggcgagtac gccccgccgt ccagccgcca ctggaagagg agcgtgttgt
     1801 ccgggctcac cgcgtcgtgc ccatcccacg tgaaagcggc atcggtgtcg ttgtcgtacg
     1861 agccgtcggc gggcccggaa aggatcgtca cgaccggcgg caggttgttc acgatgaagt
     1921 tgcgcacctc cggcgccgag gcgttgccgg ccgggtcgtg cgaccggacg cggaaggtgt
     1981 gggggccgtc ggcgagcccg gtcagcgaag tcgtggagtc ggcggacggc gtgccgaacg
     2041 cgccggcgtc gagcgatgtc gagaagagca gctcctcccg cggcacctgg ccatccattc
     2101 cggtccaggc gaaggcggcc atgtcggtgt cgagcacccc accctcggcc acgccggcga
     2161 cgatggccgc cgtcggcgct tcctggtcca cgatgaagct gcgcgcggcc ggactggcat
     2221 cttcattgcc ggcggcatcg cgggcgcgca cccggaacgt gtgcggcccg gtgccgacga
     2281 acgggaactc gccgctggtc gcgggggaaa aagcgctctc cggatcatcg tcgagctttg
     2341 ttgcgaacac gaggccggtg ttcgcgtcgg tcccctgcca ggcgaacgat acggagagca
     2401 tattggtgta cccgccgtcg cccggcccgc cggtgatggc cgtctcggga ggggtgagat
     2461 ccacggtgaa ggtccgcgtg gccaggccga ggttgccgta ggcatcacgc gcgcgcacga
     2521 cgaacgtgtg ggcgccttcc gccagggcgg tgaggttggc ggtggtgtcg gcggcgaagg
     2581 gcgtggggct gccggcgtcg atctgccacg agtattcgag cgccgccgcc ggcgtcgtgt
     2641 tgtcggtggc atggaaggtg aaagtcacgt ccggcgtgtt cacgagggca ttctcggcgg
     2701 gtccgccccc caggatgacc aggggcggga cgacgtcgat gagccatgtc tgttcggccg
     2761 gagtgggatc ggaaaggcca tccccgtcgt gcgcgcgtac taggaacgtg tgcgaaccgg
     2821 ggacgaggcc ggtcagcgtc acactggtgt ctggagcgtc cggaatgaat ggggcaccgt
     2881 cgaggctcca cgaataagtg agggcggccg tcggcgtctg gatgtcggta cccgtccagc
     2941 tgaagtgggc cgtcgtcgcg cccgtggcgg cgcccggtcc ggagaggatt tccgtgtccg
     3001 gacgattcgg gtcattgatg acatgcaggg agatcttgcg atcggagaat cggggttcga
     3061 ggtagaagcc gttcggcagg agcatgccgg tgatgttggg cgtgccggtc cgctcctcga
     3121 acgtgacgat gtcgaaggaa tcgtcgtacg agggaacgaa gccgtcgatc aggctcacgt
     3181 tgatgtcgcc cgacatgttg gcgaactgcg tggccacgag gcgatcgtat cccgtaccgg
     3241 cggtcgtgcc gccgatctcg atgtcgaggt cgccgctcgg tccctggtgg aagatgtcgt
     3301 tgaacgtcag gatccccggc gaggtgcccg gccggaccgt gccgttgttg atgaagtgca
     3361 tgggaccgac ctcgaacgtg ccggacccct ggatgacgcc cccggcctgg ttggtgaaat
     3421 tgggggcgga cccgacgctg gtcaggagct gccccggccc gatggtgatg atgccgccgt
     3481 tcgagaacga cgtccccgaa aggtcgatgg cgaagggccc cgccacgatg tcgatggttc
     3541 ccgtgttgtc ctcgacgccg ccggtggtcc gccgcagcgt cagcgggtgc tgcacggaaa
     3601 cggtgccccg gttgtcgagc agcagctcca gcttccgcgt gccccccgtg cccggcaggg
     3661 agcggatcat gccgccggtc tggttgatca gggcgccgtt ggccacggcc aggacggaat
     3721 tggcgggaat attgatcgac gacagctcga tggtccccag gttcgtgaaa ccgttggcca
     3781 cctccatccg accgtcaccc gtgttgttct gccgctccac ggaaaggagc gagccggcgg
     3841 ccgtggtcag tgcgccgttg atggcaagca gcgtgccgct caacttgaag gttccggcat
     3901 tgtcgaggga cgagttcagg accagggtgc ccggcaccgt gaccgtgcgc cccgagggat
     3961 tggtgaacgc gccgccgccg atggtgttgt ttcctccgag ggacagggcg atgtcaccgg
     4021 tcgagaggcc gttgtcgaag gtggcgttga cgttgttcaa cgacagcgtg ccggcgccgc
     4081 tgatggtgcc gccggcctta tgcaggacgc ccccggtcac gatgaagctg cgtgaggcgg
     4141 ccacgctgat cgtaccgctc gtggtgaagg atggagacgt ggacgactgg gtcaacgaga
     4201 agttgccccc cgtgatgtcg atcgtgccgc tgttgaggtg ggtggcggcg gccttcgaca
     4261 acgtcatgtt tccattggcc agagacacca ggccgcggtt gtcgagctgg caacccagga
     4321 tgcgcgtggc gaagcccgga ctgccgatgg agcggatttc gcccccgacg tcgttgatca
     4381 gggtgccgga accgacattg aacgtggagt tggagaggcc ggcggcgctc atctgcaggc
     4441 tcccctggtt ggtgaagccg ctggcgacgg tgacaacggc acccgtgttg agggacagga
     4501 tgccgccggc ggtattgctc accgctccgg tcagggagat gttggtgctg acgaatgtgg
     4561 tcgtcccctg attgatcagc ggcgcattga gcgtggcatt ggccggcagc gtgaacgact
     4621 tgccgaccgc cacggtgaag gggccgccat tgatcgtgca cgttcccgcc agggtgatgc
     4681 ccgtcacggc attggagaat ccgttgtcga acgtggccgt ggcggtggac aacgcgatcg
     4741 tccccgtccc gctgatcgat ccgccggcat cctggaagag accgccgctc acgctgaggg
     4801 tgcgtcccga gccgagggcc acgttccccg ccgaaatgaa cgtgggcgtg gtgccggatt
     4861 gaagcagggt aaagatcccg gaaccgccgg caacgagatc gaccgttccc gagttcaggt
     4921 gatcggcgcc ggccttgtcg agcgtggtcg acaccccgac ctcgaggagc ccgcggttgt
     4981 cgagctgggc caggatcgag cgcgcgccgc ccgaggccag cgaggctcgg atcgtgcctc
     5041 cgctctcgtt gacgagcgtt ccgttcgtga ccgtcagcgt cgagaccggt gcgccgccgc
     5101 tcgccgacag gtcgatcgtg ccaaggttgg tgaacccgtt ggtcacggtt atcccggccg
     5161 tgccggctga tggttcgatg acgatcgaag aggttgctga ggtcgtgagg gcgccggtca
     5221 acgccagcgc gccggtggca cgcacgctcc cctggttcga gagcgctccg ccgatcgtga
     5281 cctggttgcc gcggagcgtg gcgccggcgg ccacggtcgt gc
//
LOCUS       DASVOD010000470         2734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_603942, whole genome shotgun sequence.
ACCESSION   DASVOD010000470 DASVOD010000000
VERSION     DASVOD010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603942"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1163)
                     /gene="lpxB"
                     /locus_tag="VF720_10645"
     CDS             complement(15..1163)
                     /gene="lpxB"
                     /locus_tag="VF720_10645"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEX7879860.1"
                     /translation="MSRNAIFLVAGEASGDRLGGALAAELRRLRPGVELVGVGGPYMR
                     DAGVTLLHDYEEFAVLGIAEVIGKLPFFRRILGQFEERFRSDPPGLFIPIDFPDFNMR
                     LAARAHSAGVPVLWYVSPQVWAWRSGRVKTLAKIVDRMVVVFPFEEAIYDKAGLPVSF
                     VGHPLLERPGPVHDAATVRARLGIAAGEKLVALLPGSRRQEIRRILPPLADAGRRLAS
                     SGVRAVLSRAPSIDAATFDDALAAAGSPNLPVWSDDTASLVRAADLAVVASGTATLET
                     GLLGTPLIVVYRMAGLSWELARRLVRVEHVGLVNIALGKRVAPELLQGEVTGDDVASL
                     VSGMLRDPERLATIRTELAALPSRLGGPGASERTARLALELLDRRGQA"
     gene            complement(1160..2134)
                     /locus_tag="VF720_10650"
     CDS             complement(1160..2134)
                     /locus_tag="VF720_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7879861.1"
                     /translation="MRVGLAGCGHLGSRHAGKLAARNDLGKAHDDPSRLVLFDTDSSR
                     ASGLADQLGVSAARDYGELLDAVDAVVIAAPTVAHAELSVAALNAGRHVLVEKPIAVT
                     LAEADRMLAAARAASRQLMVGHIERFNPGVRRLEGRLFAPRFIEAHRLAGYKPRSLDI
                     DVILDLMIHDIDLVLHLVRELPFRIEAAGTPVLTGKIDIANARLSFPSGAVANLTASR
                     VSLAGTRKIRIFMPETYVSIDLATRGAQVYRLPGGAAEPVADADAFLARLQHESIEDD
                     GQDQIDAEHSAFLAASAGQAPNPVPGEDGRAALAVAMEVIGRLAEREG"
     gene            complement(2141..>2734)
                     /gene="lpxA"
                     /locus_tag="VF720_10655"
     CDS             complement(2141..>2734)
                     /gene="lpxA"
                     /locus_tag="VF720_10655"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEX7879862.1"
                     /translation="HAALGLAPQDLKYRGEITYLEIGERTVIREFATLNLATDEGKTT
                     RVGSDCLLMAYTHVAHNCVLGNQVILANAVNLAGHVTIEDNVIVGGVTPVHQFVRIGC
                     HAMVGGASRVPQDVTPYALVAGNPPVLAGLNRIGLERRGFTPEAIASLKLAYRLLFRS
                     GLLLADAVQKVRQEVPPGPEVGHLLEFVEGSQRGLTR"
BASE COUNT          427 a          964 c          957 g          386 t
ORIGIN      
        1 atgccgtcaa ccggctacgc ctggccacgc ctgtccagga gctcgagcgc caggcgggcg
       61 gtgcgctcgc tggcgcccgg cccacccagc ctcgagggaa gcgcggccag ttcggtccgg
      121 atggtcgcga ggcgctccgg atcgcgcagc atgccgctca cgagggaggc aacatcgtcg
      181 ccggtcacct cgccctggag gagctccggc gccacgcgct tgccgagtgc gatgttgacg
      241 aggccgacgt gctccaccct gaccagtcgg cgcgccagtt cccaggagag gcccgccatc
      301 cggtacacca cgatgagagg cgtgccgagc aatcccgttt cgagcgtggc ggtacccgac
      361 gccaccaccg ccaggtcggc cgcgcgcacc agtgaggcgg tgtcgtcgct ccagaccggc
      421 aggttgggcg aaccggcggc ggccagcgcg tcgtcgaacg tggcggcgtc gatgctcggc
      481 gcacggctga gaaccgcgcg cacgccggat gaggccagcc gtcttcccgc atcggccagc
      541 ggtggaagga ttcggcggat ctcctgtcgg cggctccccg gcaggagggc caccagcttt
      601 tcgccggcgg cgatgccgag ccgcgcgcgg accgtggccg cgtcgtgtac cggccccggc
      661 cgctccagca gcgggtggcc gacgaacgaa accggcagcc ccgccttgtc gtagatcgcc
      721 tcctcgaagg gaaaaaccac gaccatccgg tcgacgattt tcgccagcgt cttcacgcgg
      781 ccggagcgcc aggcccacac ctgcggactc acgtaccaga ggacgggcac gccggccgaa
      841 tgggcgcgcg cggccagcct catgttgaaa tcgggaaagt cgatcggaat gaagagcccg
      901 ggaggatcgc ttcggaaacg ctcctcgaat tggccaagga tccgacggaa gaacggcagc
      961 ttgccgatca cctcggcgat gccgaggacg gcgaactcct cgtagtcgtg gaggagcgtg
     1021 acgccggcat cccgcatgta cggcccgccc acgccgacca gttccacccc ggggcgcagt
     1081 cgcctgagct cggccgccag cgcgcccccg aggcgatcgc cggaggcctc gccggccacc
     1141 aggaagatgg cgttccggct cacccctcgc gctcggccag ccggccgatc acttccatgg
     1201 ccaccgcgag ggccgcccgg ccatcctcgc ccggcaccgg gttcggcgcc tggccggcag
     1261 aggccgccag gaatgccgag tgctcggcgt cgatctggtc ctgtccgtcg tcctcgatcg
     1321 actcgtgctg caggcgggcc aggaaggcat cggcatcggc caccggctcg gccgcgccgc
     1381 ccggcaaccg gtaaacctgc gccccgcgcg tggccaggtc gatcgacacg taggtttccg
     1441 gcatgaagat ccggatcttt cgcgtgccgg cgagcgacac ccggctggcc gtcaggttgg
     1501 ccaccgcgcc gctcggaaac gacagtcgcg cgttggcgat gtcgatcttg ccggtcagga
     1561 ccggcgtgcc ggcggcctcg atccggaacg gcagctcgcg caccaggtgg aggacgaggt
     1621 cgatgtcgtg gatcatcagg tcgaggatca cgtcgatgtc gaggctgcgc ggcttgtagc
     1681 cggccaggcg atgcgcctcg atgaagcgcg gcgcgaagag gcgcccctcg agacgccgga
     1741 cgccgggatt gaatcgctcg atgtgcccca ccatgagctg ccgggaggcg gctcgcgccg
     1801 cggccagcat ccggtcggcc tcggccagcg tgacggcgat cggcttctcc accaggacat
     1861 gccggccggc gttgagcgcc gccaccgaca gttcggcatg cgccacggtc ggcgccgcga
     1921 tgaccacggc gtccacggcg tcgaggagct caccgtaatc ccgggcggcg gaaacgccga
     1981 gctggtcggc gagtcccgag gcgcgcgaac tgtcggtatc gaagagaacc aggcgcgacg
     2041 gatcgtcgtg cgccttcccc aggtcgtttc gggcggcgag ctttccggcg tggcggcttc
     2101 ccaggtggcc gcaaccggcc aggccgaccc tcatcacccg ctagcgcgtg aggccgcgct
     2161 gcgagccctc gacgaactcg aggaggtggc cgacctcggg gcccggcggc acttcctgcc
     2221 gtaccttctg caccgcatcg gccaggagca acccggaacg gaaaaggagt cgatacgcca
     2281 gcttgagcga ggcgatggct tccggcgtga atccccggcg ttcgagcccg atgcggttga
     2341 gcccggccag cacgggcggg ttgccggcca ccagcgcgta gggcgtcacg tcctgcggca
     2401 cgcgcgaggc gccccccacc atggcatggc agccgatccg cacgaactgg tgcaccggcg
     2461 tcacgccacc cacgatgacg ttgtcctcga tggtgacgtg accggccagg ttgaccgcgt
     2521 tggccaggat cacctggttt ccgaggacgc agttgtgggc cacgtgcgtg taggccatga
     2581 ggaggcagtc cgatccgact cgggtggtct ttccttcgtc ggtagccagg ttcagcgtgg
     2641 cgaactcgcg gatgacggtt cgttcgccga tctcgagata ggtgatctcg ccgcgatact
     2701 tcaagtcctg tggcgccagc cccaacgccg catg
//
LOCUS       DASVOD010000471         3180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_500934, whole genome shotgun sequence.
ACCESSION   DASVOD010000471 DASVOD010000000
VERSION     DASVOD010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500934"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /gene="hemW"
                     /locus_tag="VF720_10660"
     CDS             <1..1210
                     /gene="hemW"
                     /locus_tag="VF720_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEX7879863.1"
                     /translation="VPANRMSATREAAIIGTMESQAQPPLTACYLHTPFCAVKCSYCN
                     FFSGVRQAGQMESWFEGLVAEIAHHDRLGRLTGRSIDTLYVGGGTPTAFDPPELARLF
                     ALLRSSLSLHPDAEITSEANPESLTDSMAETLVGLGVSRVSMGAQSFHDDELLRLNRP
                     HDAAAIVQAVRAARRAGIGSLNLDLIYGLPGQTLDRWQYSVQAALELAPDHLSCYCLI
                     LEPATPLTVEVGAGRQPRPDDGLQRDMFDWTVETLVRHGYEFYELSNFARPGRRSQHN
                     LRYWLGQPWLGLGPSAHSYLDGARGANPANLDLYRRRFTAAEPQDTFRPVAPNDLVFE
                     RVFMNLRLIEGMNLNDFQSEFGAPLDYFYPEVLPRLLSRGQLVREGQTLRLGPEWWFL
                     SDGIFTEFAP"
     gene            1207..1836
                     /locus_tag="VF720_10665"
     CDS             1207..1836
                     /locus_tag="VF720_10665"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX7879864.1"
                     /translation="MKADSSRIERFTRWLYRYRGETAAPFAVILLVLARPTWGRLAVG
                     LFLVMLGECLRLWAASHIGTHARGRTFRAPTGGMRARTGPYRLIPHPLYAGNLIVCLG
                     LLVAARAGEPWFPALFLVVFCVQYSLFMRYEGMLIRSGPANSPEGPANSPDGPANSPG
                     DGAVTPAVPPATLMPALTRAGFALEWPTLRTVMALSLVLVGLAWWWSPR"
     gene            2326..2817
                     /gene="nuoB"
                     /locus_tag="VF720_10670"
                     /pseudo
     CDS             2326..2817
                     /gene="nuoB"
                     /locus_tag="VF720_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744161.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
BASE COUNT          553 a         1118 c          967 g          542 t
ORIGIN      
        1 cgttcccgcc aaccggatgt cagcgacccg tgaggctgcg ataatcggca ccatggagag
       61 ccaagcacaa cccccattga ccgcctgtta cctgcacaca ccgttctgcg ccgtgaagtg
      121 ctcgtactgc aacttcttct ccggcgtaag acaggccggc cagatggagt cctggttcga
      181 aggactggtg gccgagatcg cccatcacga ccggttggga cgcctcacgg gacggagcat
      241 tgacacactc tatgtgggcg ggggtacccc aacggcattc gatccaccgg agctggcacg
      301 acttttcgcg ctgctacgtt cgtcgctttc cctgcatccg gacgcggaga tcacttcgga
      361 agccaatccc gaaagcctca cggactcgat ggcggagacc ctggtcgggc ttggtgtcag
      421 ccgggtgtcc atgggggcgc agtcattcca tgatgacgag cttctccgct tgaaccggcc
      481 gcacgacgcg gccgcgatcg tccaggccgt gcgggcggcg cgacgtgccg gcatcggcag
      541 tctcaacctc gacttgatct acggcctccc gggccagacg ctcgaccgct ggcagtacag
      601 cgtgcaggcc gcgctggaac tggcaccgga tcacctgtcg tgctactgcc tcatcctgga
      661 acccgccact cccctgaccg tggaggtcgg ggccggtcgc cagccccggc ccgacgacgg
      721 acttcagcgg gacatgttcg actggacggt cgagacactg gtgcgccacg gctatgagtt
      781 ctacgaactg tccaacttcg ccaggccggg ccgtcgctcg cagcacaatc tgcgctactg
      841 gctggggcag ccgtggctcg gactcggccc gtcggcccac tcctacctgg acggagcacg
      901 aggtgccaat cctgccaacc ttgacctcta ccgccggcgc tttacggcgg cggaaccgca
      961 ggacaccttt cgtccggtgg ctccgaacga cctcgtgttc gagcgcgtgt tcatgaatct
     1021 tcggctgatc gaggggatga acctcaacga ctttcagtcc gagttcggcg cgccgctcga
     1081 ctacttctac cccgaggtcc ttccaagact cttgtccaga ggacaactcg tccgggaagg
     1141 gcagaccctc cgactcggcc cggaatggtg gttcctctcc gacggcatct tcaccgaatt
     1201 cgcgccgtga aggcggactc gagccggatc gaacgtttca cccgctggct ctatcgctac
     1261 cggggggaga ctgcggcgcc attcgccgtc atcctgctcg tactggcccg accgacttgg
     1321 gggcgcctcg cggtcggcct tttcctggtc atgctcggcg agtgcctccg gctctgggcc
     1381 gccagccaca tcgggactca tgcgcggggg aggactttcc gcgcgccgac cggcggtatg
     1441 cgcgcccgca cgggcccgta tcgcctgatc ccgcatccgc tctatgccgg aaacttgatt
     1501 gtctgccttg gtctcctcgt ggcggcccgg gccggcgagc cctggttccc ggcgttgttc
     1561 ctggtggtat tctgtgtgca atacagcctc ttcatgcgct acgaaggcat gcttatccgc
     1621 agcggccccg cgaactcgcc cgaaggcccg gcgaactcgc ccgacggccc cgcgaactcg
     1681 cccggcgatg gcgcagtcac ccctgcggtt ccgcccgcca ccctgatgcc cgccctcaca
     1741 cgcgccggct tcgcgctgga gtggccgaca ctccgcacgg tcatggcact cagcctggtc
     1801 ctggtcggcc tggcctggtg gtggtcgccc cgctgaagcg gcactggtac ggagtgtgcg
     1861 gcggacggga cagcccatcg gcggacggga cagcccatcg gcggacgggg acagcccatc
     1921 ggcggacgaa acagcccatc ggcggacggg acagcccatg cgggggacgg aacagccgcg
     1981 tgcgaggggg agcggccagc aggtaagttg gacattttga ccgacctgtc ccgcgaagcg
     2041 cttgcccctg aaatcattac aggtttatcg tgaacccccg cccgatcccc cgccgcttgt
     2101 tccaactttc acaaatccat ataatcgccc gcgatcgatc gatcgacgtg gttgcctttc
     2161 tccgaacgaa gggcgggcgt tgcagaaaca caattcggca accccagcca ccggaccggg
     2221 tacccgattt cgtacctgat cgacgccagg caactggtcg cggtgatcga cggccacatc
     2281 cactccattc gggctcaccc ccggcggaac aggaaagacc cgagaatggc caagtttcag
     2341 atcgacgaca cgcccgggac ggacgacgaa aagctcgaaa aggaagtcct ttcccgcgtt
     2401 ctcgtcacca agctcgattt cgcgctcaac tgggcccggc gcaacagcct ctggccgatg
     2461 ccgatgggca tctcgtgctg cgccatcgag atgatggcca ccgccgccgc ccgctacgac
     2521 attgccaggt tcggctccga ggtgctgcgc ttctcgccgc gccaggccga cctcatgatc
     2581 acggccggca cgctcaccaa gaaaatggcg ccggtcgtcc gccgcatcta cgaccagatg
     2641 ccggaaccga agtgggtcat tgcgatgggc acctgcctct gcacgggggg gatgtaccac
     2701 acctacgcgg tcgttcaggg gctccaggaa atcgtcccgg tcgactgtta cataccggga
     2761 tgtccgcccc gccccgagaa cctcctctcc gccctgcagc acatccagaa gctgatcacg
     2821 gccggaaacg tccaccacga ccgtcgcagc gaaacgcagg ccgaatggag cctcgaccag
     2881 ctcttctacg acaagctggc ccgcctccat gcggaccagc cgggcgtttc gaacgtgcac
     2941 gagcgcccca cgcttaccgg accgcgctcg gcaacgcagt cggaactggt agcccccggt
     3001 gagccgggcg ccacctcggg accgggtgac ccgacggcga accggatcat cggccagtcg
     3061 gatgccggtg gccacggaac gcctggcgat cccaactcct cgccggccgg aaaggcatga
     3121 tccgaccaat gattcgcctc acgatgaatc gtccttcccc ggatcgacat gcgacctgct
//
LOCUS       DASVOD010000472         5718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_917892, whole genome shotgun sequence.
ACCESSION   DASVOD010000472 DASVOD010000000
VERSION     DASVOD010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_917892"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF720_10675"
     CDS             complement(<1..183)
                     /locus_tag="VF720_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879865.1"
                     /translation="MRRRIGLLVLCAATAGASCGGDDPAPQGPGLEAPFFRGVGDLPG
                     GEAKSEALGISDDGRVV"
     gene            complement(176..679)
                     /locus_tag="VF720_10680"
     CDS             complement(176..679)
                     /locus_tag="VF720_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879866.1"
                     /translation="MITIRPATTADQDSLGRFGGALMRQHHAEDPKRFIQVEHPEAGY
                     GRFLVSRLTDANSLVLVAEREGNVVGYLYADVESTNWMELRGPCGMVHDIYVDAAARR
                     SGAGHALMRAGIDWIRSQGRSQVVLSTKTGNEHAQRLFRLIGFRPTMVEMTLDEEPDQ
                     GGGPRDA"
     gene            complement(706..1359)
                     /locus_tag="VF720_10685"
     CDS             complement(706..1359)
                     /locus_tag="VF720_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879867.1"
                     /translation="MLNRRSVLVLAATLMIAAGASAARETSPTPPEVTTPLRAEFTAR
                     MKEVLSLTDAQWLELEPLITTRVQTVDAALIALESAEKPDVAGFIEGYGRMRKEFEVD
                     VMKVLTADQRGSWDAFKADFETDLVEEGAAKKLLELQAALKLTDEQVTKMRPAMSASL
                     QKKLDLFQELLEDEHINVREERRAKRTLDDLNAELWKSISKVMTVDQRNAYTRMQGQ"
     gene            complement(1551..1853)
                     /locus_tag="VF720_10690"
     CDS             complement(1551..1853)
                     /locus_tag="VF720_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879868.1"
                     /translation="MAAKRKAVRRKVAKKAVRRKVAKKAVRRKVARKAVRRKVAKKAV
                     RRKVVRKAAKRKVAKKAVKRRMVKRAVKRRVVKRAVKKAILKRAIANAMTNQEDES"
     gene            2324..2905
                     /locus_tag="VF720_10695"
     CDS             2324..2905
                     /locus_tag="VF720_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879869.1"
                     /translation="MAGQVQAGESWSRSPWRVVIWGTAGLLLLVPLVAMQFSSGWNWS
                     PFDFVAMGALLAGVCGTFELAARATVSKQYRAAVAIAVVASFLLIWMNAAAGIIGDED
                     NPANLLFGGVLLVAFLGSLGARFRATSMAPVFVATALAQAAVGVIGVVSFREEPMKLL
                     ALTGFFVGLWLVSAQLFRWAGKARVSTGRHPMA"
     gene            complement(2919..3734)
                     /locus_tag="VF720_10700"
     CDS             complement(2919..3734)
                     /locus_tag="VF720_10700"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879870.1"
                     /translation="MNPNKALWEKGDFTTIASTMRESGDAFVTSLGVSLPLRALDLGC
                     GDGTTALPLARLGADVVGIDIARNLVEAGNRRAKEAGLANLTFREGDACELKDVADGE
                     FDLAISVFGAMFAPKPFDVARELVRVTRPGARIVMGNWIPNDPTSFVSQLLRISSAFT
                     PPPPEGFISPMSWGVESHIVERFGQAGISPDRIAMTRETWNFLSPTKGPAEWIDDFRR
                     FYGPTMNAFEAAEKNGKGEELHAQLIDLATSENRNTTGGTAIGATFMRVTVQR"
     gene            complement(3754..4212)
                     /locus_tag="VF720_10705"
     CDS             complement(3754..4212)
                     /locus_tag="VF720_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7879871.1"
                     /translation="MAGKVAKKLTRVSRKAVTAKPKLLSGGNPQIAKADGDAPVKAYI
                     AAMPGWKRQAGARLDALIERTVPGVRKAVKWNSPFYGVEGQGWFLSFHCFTKYIKVAF
                     FRGAALRPLPPGESRSPDTRYLDIHEDDELDEKLVARWIRQASKLPGWTP"
     gene            4393..5121
                     /locus_tag="VF720_10710"
     CDS             4393..5121
                     /locus_tag="VF720_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879872.1"
                     /translation="MSRLLSCKTFALTLGLVALASVAGADIRGTGYTITASNADGSAS
                     YSIPAPTSHEAYWGWTTTERIEMRDPLSGNLVATLNPNNEGCNVHYVEDPVVALGFAV
                     QAGAAPTLFMISSGTLAFAPIPAAEARSSGSYTLTDITGDGATLTPLGAKSYHSLYNG
                     PTVFSDLVSGFASPAFTSTPASESLPAGPGFIPIGPAVDMRAEIDFVLSPFDLASGTT
                     VYVIQQRPTPVIDATWSRIKSLTQ"
     gene            complement(5196..>5718)
                     /locus_tag="VF720_10715"
     CDS             complement(5196..>5718)
                     /locus_tag="VF720_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7879873.1"
                     /translation="GFGIGGPINATLITGNTLAIGPHEVTGILTRLPDPGNGAFDAED
                     VAGSIGTGVLKRFQLRVDYPGRTLWLKPGTWFEEGDACDRAGLWLARRGEELEVVDVV
                     SGGPAESAGLSVGDRIVRVGGSPARSIMLPDFRRRLATDPAGTRIEFVVRNSKGTTSR
                     QLVLRDLLPGAEH"
BASE COUNT          902 a         1908 c         1811 g         1097 t
ORIGIN      
        1 caccacccgc ccgtcgtcgg aaatcccgag cgcttcgctc ttcgcctctc ccccggggag
       61 gtcgccgacg ccccggaaga aaggcgcttc caggccgggg ccctgcggcg cggggtcatc
      121 gccgccgcac gacgccccgg ccgtggcggc gcaaaggaca aggagtccga ttcgcctacg
      181 catcgcgggg tcctcctccc tggtcgggct cctcgtccag cgtcatctcc accatggtgg
      241 gccggaagcc gatcaagcgg aaaaggcgct gggcgtgttc gttgccggtc ttggtcgaga
      301 gcaccacctg gctccttccc tgcgagcgga tccagtcgat gccggcccgc atcagggcgt
      361 gcccggctcc cgatcggcgc gccgccgcat ccacgtagat gtcgtggacc atgccgcacg
      421 gcccgcgcag ctccatccag ttggtcgatt cgacatcggc ataaaggtat ccgacgacat
      481 ttccctcgcg ctcagccacc aggaccaggc tattggcgtc ggtcaggcgc gacaccagga
      541 accgcccgta gccggcctcc ggatgctcca cctggatgaa tcgtttcgga tcttcggcgt
      601 ggtgctgccg catgagcgcg ccgccgaagc ggccgaggga gtcctggtcc gcggtcgtgg
      661 ccggtcggat cgtgatcatc cggaagatcg tgcggggccc gggagctact gcccctgcat
      721 cctggtgtag gcgttgcgct ggtccaccgt catcaccttg gagatcgact tccagagttc
      781 ggcattcagg tcgtccagcg tgcgcttggc acggcgttcc tcgcgaacgt tgatgtgctc
      841 gtcctcgagc agctcctgga acaggtccag tttcttctgg agcgaggcgg acatcgccgg
      901 ccgcatcttc gtcacctgct cgtcggtcag cttgagcgcc gcctggagtt ccagcagctt
      961 cttcgcggcg ccctcctcga ccagatctgt ctcgaagtcc gccttgaagg cgtcccacga
     1021 accgcgctga tccgcggtga ggaccttcat cacgtcaacc tcgaattcct tccgcatccg
     1081 gccgtacccc tcgatgaatc cggccacgtc cggcttttcc gcggattcga gggcgatcag
     1141 ggcggcatcg accgtttgga cacgggtcgt gatgagtggc tcgagctcga gccactgggc
     1201 gtcggtcaag ctcagcactt ccttcatgcg cgccgtgaac tcggccctga gaggagtcgt
     1261 gacttccggc ggggtcgggg aggtttccct ggccgcggac gctccggcgg cgatcatgag
     1321 ggtggcggcg agtaccagga cagaacggcg gttcaacatc agcgcgctcc ggggcttggg
     1381 agatgcggga aacatccgga agcatatcac cggcaccggg cccgccgttg tgcggggcgg
     1441 ggaaacggaa agggccgggg atcaatcccc ggccctttcc atctgtcgac ttgctaccgt
     1501 ctgtctggac ggtaccgccc tgtcatgccg gcggtgtcac gacctcaggc tcaggattcg
     1561 tcttcctggt tcgtcatcgc gttggcgatg gcgcgcttca ggatggcctt cttgaccgcc
     1621 cgcttcacga cgcgacgctt gacggcccgc ttgaccatcc ggcgcttcac ggccttcttg
     1681 gcaaccttgc gcttggcggc cttcctgacg accttgcggc gaaccgcctt cttcgcgacc
     1741 ttgcgccgga ccgccttcct ggcgaccttg cgccgaaccg ccttcttcgc aaccttacgc
     1801 cggaccgcct tcttcgcgac cttgcgcctt acggccttcc gtttcgctgc cacagcaaag
     1861 actcccttcc gtggtccaaa agcgacacct ggactcggca ctcccacacc ggccgccgac
     1921 tgaactcgct cggggccccg gtcgggagac ctcacattcg tggctctaca gcgagtttca
     1981 tgccacgagc gagtaaattg gggccgaccg tgcaagttat tagaaaacaa tgatttatgt
     2041 ccgaggtgat gccgtccgga tacaggaagt cctcgtgaac gccggtccta agtgtccaat
     2101 ttcaatgcag ttggagagtg tgagtcgtta actgattgca gtttatggag ttacgaaaac
     2161 ggccagatcg tcgttttatt ggacatgtca accaaatggt cagttgcgcc caggcattct
     2221 cctgcagaaa atcctgttgc gttctcttta tgacgtaaag tacattatgg ccggagtcga
     2281 aaacgtgaac cggtacccgg aacatcaccg cggaggcaat gccatggcag gtcaggtgca
     2341 ggcgggagag agctggagca ggagcccctg gagggtggtc atctggggaa cggcggggct
     2401 gctcctgctg gttcccctgg tggcgatgca gttcagcagc ggatggaact ggagcccgtt
     2461 cgatttcgtg gcgatgggcg ccctcctggc cggtgtgtgc ggcacgttcg aactggcggc
     2521 gcgtgccacc gtcagcaagc agtaccgggc tgccgtcgcc atcgcggtcg tggcttcgtt
     2581 cctcctgatc tggatgaacg cggccgccgg catcatcggc gatgaagaca acccggccaa
     2641 cctcctgttc gggggcgtgc tgctggttgc ctttctcggt tcgcttggag cccgcttccg
     2701 cgccacgtcc atggctcccg tctttgtggc aacggcgctc gcccaggcag cggtcggcgt
     2761 gatcggcgtg gtgtctttca gggaggaacc gatgaagctg ctcgccctca ccgggttctt
     2821 cgtggggcta tggctcgtct ccgcccagtt gtttcgctgg gcggggaagg ctcgggtttc
     2881 aacagggagg catccgatgg cctgatcgaa cgggccgatt accgctgcac cgtcaccctc
     2941 atgaacgtgg ccccgatggc cgtgccgccc gtggtgtttc ggttctccga ggtggccagg
     3001 tcgataagct gggcgtggag ttcctctccc ttgccgttct tttcggccgc ctcgaaggcg
     3061 ttcatcgtcg ggccgtagaa gcggcggaag tcgtcgatcc actcggccgg gcccttggtg
     3121 ggtgagagga agttccaggt ctcgcgggtc atggcaatcc ggtccgggga aatgccggcc
     3181 tggccgaagc gctcgacgat gtgcgattcc acgccccagg acatcgggct gatgaacccc
     3241 tcgggcggcg ggggagtgaa ggccgaggaa atgcggagca actgcgagac gaacgatgtc
     3301 gggtcgttcg gaatccagtt tcccatgacg atgcgggccc ccggcctcgt tacgcgcacc
     3361 agttcccggg ccacatcgaa tggtttcggc gcgaacatcg cgccgaaaac cgatatggcc
     3421 aggtcgaact ccccgtcggc cacgtccttc aactcgcagg catcaccttc ccggaacgtg
     3481 agattggcaa gaccggcctc ttttgcccgc cggttgcccg cctccaccag gttccgggcg
     3541 atgtcgatgc cgaccacgtc cgcaccgagc cgggccagcg ggagcgcggt ggtgccgtcg
     3601 ccgcatccga ggtcgagcgc gcgcagcggc aggctgacac cgagcgacgt gacgaaggcg
     3661 tcgcccgact cccgcatcgt cgacgcgatt gtcgtgaaat cgcctttttc ccacagggcc
     3721 ttgttcggat tcatgtcggt tccttcctgg ttgctatgga gtccagccgg gaagtttcga
     3781 ggcctggcgg atccaccttg ccacgagctt ctcgtcgagt tcgtcgtcct cgtggatgtc
     3841 gaggtagcgc gtgtcggggc ttcttgattc gccgggtgga aggggacgga gcgccgcgcc
     3901 ccggaagaaa gccaccttga tgtacttggt gaagcagtga aagctgagga accacccctg
     3961 cccctccacg ccgtagaacg gtgaattcca tttcacggcc ttgcgcaccc cgggcaccgt
     4021 ccgctcgatg agcgcgtcga ggcgggcgcc cgcctggcgc ttccaccccg gcatggctgc
     4081 aatgtaggcc ttgaccgggg cgtcgccatc ggccttcgcg atctggggat tgccgcccga
     4141 caggagtttc ggtttggcgg tgacagcttt ccttgacacc ctggtcaatt tcttcgcgac
     4201 cttgccggcc atgatcgtca ctccgtgggt gggcacactc tatgctggtt ggcgcgacct
     4261 cgcccggata cccgggccgg tcgaattccc tgccgaatac tatgggcaat cgggggaaat
     4321 caggagtagg ctgacgcacc cccctctcct gtccatttgt tgaacgacca tccatgtagg
     4381 gaggacaaag ccatgtcgcg actgctttcg tgcaagacct tcgcgctcac cctcgggctg
     4441 gtggcccttg cgagtgtggc cggagccgac atccgcggaa cggggtacac gatcacggcc
     4501 tccaacgccg acgggtcggc cagctactcg attccggcgc cgacgtccca cgaggcgtac
     4561 tggggttgga cgaccaccga gcgcatcgag atgcgcgatc cgctgtccgg aaacctggtg
     4621 gccacgctga acccgaacaa cgagggatgc aacgtccact acgtggaaga ccctgtcgtg
     4681 gccctgggct ttgcggtcca ggccggcgcg gctccgacgt tgttcatgat ttcgtccggc
     4741 acgctggcct tcgcgccgat tccggccgcc gaggcccgga gctcgggctc gtacaccctg
     4801 accgacatca ccggtgacgg cgccacgctc acgccgctcg gcgccaagtc gtaccactcg
     4861 ctctacaacg gccccacggt cttctccgac ctggtcagcg gattcgcttc tcccgccttc
     4921 acttccaccc ccgcgtccga atcgctgccg gcgggcccgg ggttcatccc gatcggcccg
     4981 gctgtcgaca tgcgcgccga gatcgatttc gtcctgtcgc cgttcgacct ggccagcggc
     5041 accacggtgt acgtcatcca gcagcgtccg acgccggtca tcgacgccac ctggagccgg
     5101 atcaagtcgc tgacgcagta gcacacgtca atcgcagaga tcgcgccgcc cgggtcatcg
     5161 gatccgggcg gcgttttttt tcgccgggtg tgccttcagt gctcggcacc cggcaacaag
     5221 tcgcgcagca cgagctggcg cgacgtagtt cccttgctgt tccggacgac gaactcgatc
     5281 ctcgtccccg ccggatcggt ggccaggcga cgacggaagt cgggaagcat gatgctcctg
     5341 gcgggcgatc cgccgacgcg aacgatccgg tcgccgaccg atagaccggc tgactcggcc
     5401 ggaccaccgc tcaccacgtc cacgacctcc agttcctcac cccgtcgggc cagccagaga
     5461 ccggcccggt cgcacgcgtc cccctcttcg aaccatgtcc ccggtttcag ccagagcgtc
     5521 cggcccggat agtccacgcg gagctggaag cgcttcagca cgccggttcc gatgctcccc
     5581 gccacgtcct ccgcgtcgaa ggcgccgttc ccgggatccg gaaggcgggt aaggatgccg
     5641 gtcacctcgt gcggcccgat cgccagcgta ttccccgtga tgagcgtggc gttgatggga
     5701 cctccgatgc cgaagccg
//
LOCUS       DASVOD010000473         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1025041, whole genome shotgun sequence.
ACCESSION   DASVOD010000473 DASVOD010000000
VERSION     DASVOD010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025041"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF720_10720"
     CDS             <1..426
                     /locus_tag="VF720_10720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879874.1"
                     /translation="ETIELGGSPAAVQKELVVASWSAGSGCDAVRVVPNPYRGGADWD
                     LGVSDCDPTGTKIAIRSLPDGWTRLRIYSLTGDLIVEADPADSRVVGTCEVSGGSRSE
                     GTFYWDLITRSGQNVVSGVYLYVVDAPGGSCRGRFVVIR"
     gene            668..1801
                     /gene="recA"
                     /locus_tag="VF720_10725"
     CDS             668..1801
                     /gene="recA"
                     /locus_tag="VF720_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008518016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX7879875.1"
                     /translation="MSSNNPSNPATSKEKQKAFDNAIVNIERQFGKGSIMKLGGPDSA
                     RARVASISTGSIGLDAALGIGGVPRGRIIEIFGPEASGKTTLTLSIIAQAQKIGGNCV
                     FIDAEHALPAEFAEMLGVNLENLHVSQPDTGEEALEIADQLVRSGAIDVVVIDSVAAL
                     VPKAEIEGEMGDSHVGLQARLMSQALRKLTGSISKSNTCVIFINQIRMKIGVMFGNPE
                     TTTGGNALKFYSSVRLDIRRIASIKSGEESIGNRCKVKVVKNKVASPFRQAEFDLYFD
                     EGISKAGEILDIGVEKGIIEKSGTWMSYGSERLGQGRENARQFLKNNPDIMAELDAKV
                     RTALGIGEAAPVDPETNPSAEIESALEEAASGARKPGAPKPKS"
     gene            2036..>2346
                     /locus_tag="VF720_10730"
     CDS             2036..>2346
                     /locus_tag="VF720_10730"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HEX7879876.1"
                     /translation="MKAADFDPVLDRLTEVGLIDDLEYARVYLERRAAGKPRGARLLR
                     NELMAKGVPGGLIDQALAERKDDHDPAEDAVKALRPKVGTLSKLDSRSRDRKAWEFLA
                     RR"
BASE COUNT          430 a          737 c          808 g          371 t
ORIGIN      
        1 gaaacgatcg agctgggggg aagtcccgcc gccgtccaga aggagctggt cgtggcctcc
       61 tggtcggcgg gatccggctg cgacgccgtt cgagtcgttc ccaaccccta ccgtggcggg
      121 gccgactggg atcttggcgt atcggactgc gatccgacgg gcaccaagat cgccattcgc
      181 agcctgccgg atgggtggac gcggctccgg atctattcgc tgaccggcga cctgatcgtc
      241 gaggccgatc ctgccgattc gcgggtcgtt ggtacctgcg aagtcagcgg gggaagccgg
      301 tcggagggca ccttctactg ggacctgatc acccgtagcg ggcagaatgt cgtgtccggg
      361 gtgtacctct acgtggtcga cgcgcccggc gggtcgtgcc gggggcggtt cgtggtgatc
      421 cggtaagcgc agggatgggg tcatggaccc ggggtgccgc tgggccggcc cgactcgttt
      481 tactcatcaa aaaactacta gacatccaga tagtttcgtg ggaggatgcg ggcatggcgg
      541 tcaggtgcca gccctgatct tctggacacg agtgtcccgg tcgggcccgt catgatgtcg
      601 cgtttgacag ctcgcgcgac atcgacctaa cgtcgttcac ggaccacccg tcgcgaggag
      661 aatacgcatg tcgtcgaaca atccgtcgaa tcccgccacc agcaaggaaa agcagaaggc
      721 cttcgacaac gccatcgtca acatcgagag gcagttcggg aagggctcga tcatgaagct
      781 gggtgggccg gattcggcca gggcccgggt ggcgtccatc tccaccggat cgatcggcct
      841 ggacgcggca ctcggcatcg gcggcgtccc gcgcgggcgc atcatcgaga tcttcgggcc
      901 ggaagccagc ggcaagacca cgctcaccct ctccatcatc gcccaggccc agaagatcgg
      961 cggcaactgc gtcttcatcg acgccgagca cgccctgccg gccgagttcg ccgagatgct
     1021 cggcgtcaac ctcgaaaacc tgcacgtcag ccagcccgac acgggcgaag aggccctcga
     1081 gatcgccgac cagctcgtcc gctcgggcgc catcgacgtg gtcgtcatcg actcggtggc
     1141 ggcgctcgtg ccgaaggccg aaatcgaggg ggaaatgggg gacagtcacg tcggcctcca
     1201 ggcccgcctc atgagccagg cgctccgcaa gctcaccggc tccatctcga agtcgaacac
     1261 ctgcgtcatc ttcatcaacc agatccgcat gaagatcggc gtcatgttcg gcaacccgga
     1321 aacgaccacg ggcggcaacg cgctcaagtt ctactcgtcg gtccgcctcg acatccgccg
     1381 catcgcctcg atcaagagcg gcgaggaatc catcggcaac cgctgcaagg tcaaggtcgt
     1441 caagaacaag gtggcctctc ccttccgcca ggccgagttc gacctctact tcgacgaagg
     1501 gatcagcaag gccggcgaaa tcctcgacat cggcgtcgaa aaggggatca tcgagaagag
     1561 cggcacctgg atgtcgtacg gcagcgaacg cctcggccag ggacgcgaaa acgcccgcca
     1621 gttcctcaag aacaacccgg acatcatggc cgagctggac gccaaggtgc gcaccgcact
     1681 cggcatcggg gaagcggcgc ccgtcgatcc ggaaacgaac ccctccgcgg aaatcgagtc
     1741 ggcgctggaa gaggccgctt ctggggcccg gaagccgggc gcgccgaagc cgaagagctg
     1801 acgggtcggc ggtcggggtc ggtaggcgag gtcggtcgtg attcgtcacg agcgggaatc
     1861 ccgggagccc cgggattttg aggacgcggg agatgacacc ggttcggggg gcagtgcgcg
     1921 ggcggccgcg gtgctgtcgc cggatcccga tgtcatccgg gaggcgtgcc tgcgtgcgtt
     1981 ggaacgacgc cgtcgcaccc gttcggagtt ggccaccctg ctgcgccgga aaggcgtgaa
     2041 ggccgccgac ttcgatcccg tgctagaccg attgaccgaa gtcggcctca tcgacgacct
     2101 ggaatatgcc cgtgtgtatc tcgagcgccg tgcggccgga aagccgcgcg gcgctcgctt
     2161 gttgcggaat gagttaatgg ccaagggggt tccggggggg ctgatcgacc aggcgctggc
     2221 cgagcggaag gacgaccacg atccggcgga ggatgcggtg aaggcgttgc ggccgaaggt
     2281 gggcactctc tccaagctgg attcgcgctc gcgggatcgg aaggcttggg agttcctcgc
     2341 gcggcg
//
LOCUS       DASVOD010000474         6451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_971194, whole genome shotgun sequence.
ACCESSION   DASVOD010000474 DASVOD010000000
VERSION     DASVOD010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971194"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF720_10735"
     CDS             complement(<1..925)
                     /locus_tag="VF720_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879877.1"
                     /translation="MNRRTFLIRAGASGAALLASGTWQPRPLEAASTLTAELAGLVHD
                     LETRFPYAYALYSRDETLNFELGDDGSRLTTQGPNEGIVAGIWDGAMIREEATSLADA
                     AGIARLRERLMALPPPLSKEGAPDPGPALEKEWIEAGRVPIGSRTIAQRVDELSALRE
                     SVKKRSAALANVRMESLQGETERIFVNRTRTLHQRLSRAGVGGVAIARNGDGKRGFLG
                     IRFRGVGGLEHAALSEPVIASWVERAERLAGAPSPEPGEMFVVSEPNVTGVVAHESFG
                     HGVELDMFVKGRARAAEFIDKPVGSKLVNISD"
     gene            994..2154
                     /locus_tag="VF720_10740"
     CDS             994..2154
                     /locus_tag="VF720_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879878.1"
                     /translation="MNNLPKGLPGAIRWVIVITLLVAWAAPTRATGIPSFPQYAGILY
                     VVGTQELDPGFTTHAETVAATGIMIGGATHVFPNQAAFEMTRHRDNGPGSAPDDPLAL
                     RRLVAEAAPGCTLGLYINAMQVPLKQPRSGQFVYKPMELALFNWITAHDSWLRRAGNG
                     KIVEGKRGWGVFDVRAPGFAEFFGSLVRRYVAEYGITRVLIDETHSTLDHLPGHADIA
                     TMPSGAEQAAGMRAMLKAASGGDTARCIWNGHLTRIMAPESYGGRMIQEAAASPSEVV
                     ISSMREAMAVVTASPRLVMLEQRGSTPAQRQYLACLATLNDGQSGCGADDFTTPIQIP
                     YPAGSWGRPLEYVNGTSTTPVVTRFGDVETRELEFATIGVNRATKTAIVVPR"
     gene            complement(2158..3321)
                     /locus_tag="VF720_10745"
     CDS             complement(2158..3321)
                     /locus_tag="VF720_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009824731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX7879879.1"
                     /translation="MTRRPTHPVIGTRVLILFLGLWALPASTVRGHDFGVTPIIITVT
                     DAGLDVRLDLHPTDLLQNVLGDESGRQVFDNTAELTRHADRIRSYVLPRVKLAVDGSP
                     LILVPQPDWPPPGLAMTRVDSTGATVPASLPLTLTGMLPPGAAQLTMSFLLYGEGEFV
                     PLFDIVIKDTAGTARRTEFLRVNQSATVPLAPFREHTAREKSVGRVFGDFLVLGFTHI
                     VPLGLDHILFVLGLFLLGGGFRSLLKQVTAFTVAHSLTLALAMLGILRLPSRVVEPLI
                     ALSIAYVAIENLYRRDVNRRRWLVVFGFGLIHGLGFAGVLEELGLPPGRFLPALIGFN
                     LGVEGGQLTVLLLATLGTLPFRHREWYRPWVARPACLVIAGVGFFWAVQRVMG"
     gene            3406..4410
                     /locus_tag="VF720_10750"
     CDS             3406..4410
                     /locus_tag="VF720_10750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7879880.1"
                     /translation="MSYLSIGLLVCLMTLVAHWVLAARRPENANPSRAGGIIVAAAAL
                     GLGFLATRLPIGAAHLSATGLTPVLAGAGLIALIGWLDDHHPLPPLLKLAGQVAAAAI
                     TYGLGLSAGFLPPGLINAALVIFCLVGGANALNLVDGVDGLAGAVSLIAAATIFAVAR
                     NAGNIEGAVMAAALAGGMLAFLWQNLPPARVYLGDAGSNFVGFTLAALPLLLSSGPRG
                     FEHFSAAMLLLAVPIVETATSIGRRLAAGRSPLQRDRQHIHHRLLRQGWTIGAVVGLG
                     TGITAALGALVLLGRDVLEGPASDLRTLINWSLLLVLLVVSSLYVGWHAGRLASSSKR
                     "
     gene            4376..5344
                     /gene="corA"
                     /locus_tag="VF720_10755"
     CDS             4376..5344
                     /gene="corA"
                     /locus_tag="VF720_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HEX7879881.1"
                     /translation="MPVVSRVLRNGSESQVDGLPDDLADILADPAARLWVDLFTIDIP
                     TLEEMGRRFNFHPLAIEDCYHNIQRPKVDEYPHYLFFSFHSIDESGDGEIAMREIDGF
                     LGPNYLVTVHEEASKEIEALLERCGRNQSTMERGSDYLFYSLLDLLVDTHFPVLDLIQ
                     DRIETAESQLFDNPDKNTLESIFLIKKELLSVRRIVGPQRDIVNALGSRGYAVISDAT
                     RLYLRDVQDHLIRLTELVETYRDLVNGAMDAYWTQSQQKLNEVMKVLTVIGTVNLPLT
                     LIAGLWGMNFDRIPWAHDPLGFWYIFGLLILVVVGMLALFRAARWL"
     gene            5472..6098
                     /locus_tag="VF720_10760"
     CDS             5472..6098
                     /locus_tag="VF720_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7879882.1"
                     /translation="MIRSLLRSAAPALVALALAGSANAAAETWDIDTSHSNVGFTVRH
                     NVIAKVYGRFSEFEGTLALDPANLGATVVDVTIQAASVDTDNEKRNNHLKSPDFFDVA
                     KYPTITFKSTAVTPKDKETGTLKGDLTINGVTKPVELAYTVIGFTDAWGGQRAGFEAT
                     GSINRKDFNVNWNKTLDNGGLVVSDKVDITLQIEAVKKKPEEAKPAGK"
BASE COUNT         1110 a         2215 c         2076 g         1050 t
ORIGIN      
        1 cgtcgctgat gttcacgagc ttcgagccga ccggcttgtc gatgaactcc gccgcccggg
       61 cccgcccctt cacgaacatg tcgagctcca cgccgtgccc gaacgactcg tgcgccacga
      121 cccccgtcac gttcggttcg gataccacga acatttcgcc cggctcggga gacggggcgc
      181 cggccagccg ttcggcccgt tccacccagc tggcaatgac gggttcggac agcgccgcat
      241 gctcgagccc ccccacgccg cggaagcgga ttcccaggaa gccgcgcttc ccgtcgccat
      301 tcctcgcaat ggccacaccc cccacgcccg cccgcgacag tcgctggtgg agcgtgcgcg
      361 tccggttcac gaagatccgc tcggtttctc cctggagcga ctccatccgc acgttggcca
      421 gcgccgcgct ccgcttcttg accgactcgc gcagggccga cagctcgtcc acacgttggg
      481 cgatggtgcg gcttccgatc ggcacgcgcc cggcctcgat ccactccttc tcgagcgccg
      541 gccccggatc gggcgccccc tctttcgaca gcgggggagg gagtgccatc agccgttcgc
      601 ggaggcgggc gatcccggcg gcatcggcca gcgaggtggc ctcctcgcgg atcatcgcgc
      661 catcccagat cccggccacg atcccctcgt tcggcccctg cgtcgtgagg cgcgagccgt
      721 catccccgag ttcgaaattg agcgtctcgt cccgggaata gagggcgtac gcgtacggaa
      781 aacgcgtctc gagatcgtgc accagcccgg ccagttcggc cgtgagcgtg gaagccgctt
      841 cgagtggtcg cggctgccag gtaccggagg caagcagggc ggcgccgctt gcgccggccc
      901 ggatcaggaa ggttcgtcgg ttcatggaat cgggaagtct atcgcagaaa ggaggcacgc
      961 accaaagtgc acatcaacgt agaattcggg tcgatgaaca acctgccaaa gggccttccg
     1021 ggagccatcc gatgggtgat cgtcatcacc ctcctcgtgg cgtgggcagc gccgacccgg
     1081 gcgaccggca tcccgtcatt tcctcagtac gcgggcatct tgtacgtggt tggcacgcag
     1141 gaactcgacc cgggcttcac cacgcacgcc gagaccgtgg ccgccacggg gatcatgatc
     1201 ggcggggcca cccacgtctt cccgaaccag gccgccttcg aaatgacccg ccaccgcgac
     1261 aacggcccgg gttccgcgcc cgacgacccg ctggccctgc ggaggctcgt cgccgaggcg
     1321 gctccgggct gcacgctggg gttgtacatc aacgccatgc aggtgccgct caagcagccc
     1381 cgttcaggac agttcgttta caagccgatg gaactcgcgc tcttcaattg gatcaccgct
     1441 cacgattcat ggcttcgccg cgccggcaac gggaagatcg tggaagggaa gcgcggctgg
     1501 ggcgtgttcg atgtcagggc gcccgggttc gccgaattct tcggttcgct cgtccggcgg
     1561 tacgtggccg aatatgggat cacccgggtc ctcatcgacg agacgcattc cacgctcgac
     1621 cacctgcccg gccacgccga catcgcgacc atgccgagcg gtgccgagca ggcggccgga
     1681 atgcgggcga tgctcaaggc cgccagcggg ggagataccg ctcgctgcat ctggaacggc
     1741 cacctgaccc gcatcatggc gccggaatcg tatggcggcc gaatgatcca ggaggcggcg
     1801 gcatcgccct cggaggtcgt gatctcatcc atgcgggaag cgatggccgt cgtaacggcg
     1861 tccccgcggc tcgtcatgct cgagcaacgc ggttccaccc ccgcccagcg ccagtacctg
     1921 gcctgcctcg ccacgctgaa cgacggccag agtggctgcg gcgcggacga cttcaccaca
     1981 ccgatccaga taccgtaccc cgccggctca tggggacgcc cgctcgagta cgtgaacggc
     2041 acctccacga cgccggtggt cacccggttc ggcgatgtcg agacgcgcga actggaattc
     2101 gccacgatcg gggtgaaccg ggccacgaaa accgccatcg tcgtaccacg ctgacgtcta
     2161 tcccatcacc cgctgtaccg cccagaaaaa cccgaccccc gcgatgacca ggcacgccgg
     2221 ccgcgccacc cacggtcggt accactcgcg gtggcggaag ggaagcgtgc ccagcgtggc
     2281 gaggaggagc acggtcagct gccctccctc cacgcccagg ttgaacccga tcagcgccgg
     2341 caggaatcga cccggcggga ggccgagttc ctcgaggacc ccggcaaatc cgaggccgtg
     2401 aatcaacccg aagccgaaca ctaccagcca gcgccggcgg ttcacgtcgc ggcggtagag
     2461 gttttcgatg gccacgtagg cgatcgagag ggcgatcaac ggttcgacca cccgcgaggg
     2521 gaggcgcagg atcccgagca tggccagcgc cagcgtgagg gagtgcgcca ccgtgaaggc
     2581 ggtcacctgc ttgaggagcg agcggaagcc gccgccgagc aggaagagtc ccagcacgaa
     2641 aaggatgtga tcgagcccga gcggcacgat gtgggtgaag ccgaggacca gaaagtcgcc
     2701 gaagacacgt cccaccgact tttcgcgggc ggtgtgctcg cgaaacgggg ccagcggcac
     2761 agtggcggac tggttcaccc ggaggaattc ggtacggcgg gcggtgcccg ccgtatcctt
     2821 gatgacgatg tcgaaaagcg ggacgaactc gccctctcca tataacagga acgacattgt
     2881 cagctgcgcg gctcccggcg ggagcatccc ggtgagggta agcggcaggc tggccgggac
     2941 ggtggcgccg gtcgaatcga cccgggtcat ggcgagcccc ggcggcggcc agtctggctg
     3001 gggcacgagt atcagcggtg agccgtccac cgccagtttc acgcgtggga ggacgtagga
     3061 ccggatccgg tcggcatggc gggtcagctc ggcggtgttg tcgaagacct ggcggcccga
     3121 ctcatccccc aggacattct ggaggaggtc ggtggggtgg agatcgaggc ggacgtccag
     3181 cccggcgtcc gtcaccgtaa tgatgatcgg ggtgacgccg aaatcgtgcc cccggacggt
     3241 ggaggccggg agcgcccaaa gccccaggaa aaggatcagc acccgggtgc cgataacggg
     3301 gtgggtgggg cgccttgtca tcacgggcac cgtcgtttag cttcaatgag cggccgctcc
     3361 agggcggtcg cgtcgcggta atccaacacg tcgggagaat agcgtttgtc gtacctgagc
     3421 atcggtctcc tcgtctgtct catgaccctg gtggcgcact gggtgctggc ggcccgtcgt
     3481 cccgaaaacg ccaacccgtc gcgggccggc gggatcatcg tggcggccgc ggccctcggg
     3541 ctcggattcc tggccacgcg ccttccgatc ggcgccgcgc acctctcggc caccggcctc
     3601 acgccggtcc tggccggggc gggcctcatc gccctgatcg gctggctcga cgaccaccac
     3661 ccgctgccgc cgctcctcaa gctggccggc caggtggcgg ccgccgccat cacctacgga
     3721 ctgggtctgt cggccggatt ccttccgccg ggtttgatca acgccgcgct ggtcatcttc
     3781 tgcctggtcg ggggtgccaa cgccctgaac ctggtggacg gcgtggatgg gctggccggc
     3841 gcggtgtcgt tgatcgccgc cgccacgatc tttgcggtgg cccgcaacgc cggcaacatc
     3901 gagggggccg tcatggccgc ggcgctggcc ggcgggatgc tggcgtttct ctggcagaat
     3961 cttccacccg cgcgcgtcta cctgggcgat gccggaagca acttcgtcgg cttcacgctg
     4021 gccgcgcttc ctttgttgtt gtcgagtggc ccccgtggct tcgagcactt ctcggcggcc
     4081 atgctccttc tggccgtgcc gatcgtggaa acggccacct ccatcggccg ccgactggcc
     4141 gccggtcgga gcccgctcca gcgcgaccgg cagcacatcc accaccggct gcttcgccag
     4201 gggtggacga tcggcgcggt ggtgggcctc ggcaccggca tcacggccgc gctcggggcc
     4261 ctggtgctcc tcggccgcga cgtgctcgag gggccggcct cggacctccg cacgctgatc
     4321 aactggtcgc tgctgctcgt cctgctcgtc gtcagctcgc tgtacgtcgg ttggcatgcc
     4381 ggtcgtctcg cgagttcttc gaaacggtag cgaaagccag gtcgacgggc tgccggacga
     4441 cctggccgac atcctcgccg accccgcggc ccgcctctgg gtggacctct tcacgatcga
     4501 cataccaact ctcgaagaga tggggcgccg cttcaatttc cacccgctgg ccatcgagga
     4561 ctgctaccac aacatccagc gccccaaggt ggacgagtac ccgcactacc tcttcttctc
     4621 gttccactcc atcgacgaat cgggggatgg cgagatcgcg atgcgcgaga tcgacggatt
     4681 cctcggcccc aattacctgg tcacggtgca cgaggaagcc agcaaggaaa tcgaggccct
     4741 cctcgagagg tgcgggcgca accagtccac gatggagcgg gggagcgact acctcttcta
     4801 ctcgctcctc gacctcctgg tcgacaccca ctttcccgtg ctcgatctga tccaggaccg
     4861 catcgagacg gccgaaagcc agttgttcga caacccggac aagaacacac tggagtcgat
     4921 cttcctcatc aagaaggaac tcctctcggt gcgccggatc gtgggaccgc agcgcgacat
     4981 cgtgaacgct ctcggctcgc ggggctacgc ggtgatcagc gatgccaccc gcctctacct
     5041 gcgcgacgtg caggaccacc tcatccgatt gaccgaactg gtggagacct atcgtgacct
     5101 cgtgaacggc gcaatggacg cctactggac gcagtcgcag cagaagctga acgaggtgat
     5161 gaaggtgctc acggtcatcg ggacggtgaa cctccccctg acactgatcg ccggtctttg
     5221 ggggatgaac ttcgatcgga taccatgggc gcacgacccc ctgggcttct ggtacatctt
     5281 cgggctcctc atcctcgtgg tcgtgggcat gctggccctg tttcgggccg cccgctggct
     5341 ctaagtcaca tcaagtcctt acagtggctt gagttacgtt atcggaaacg aaatccggaa
     5401 cagccgatct tattcgttgt tccaacacac gctaatgtcc gactgacacc aacctgggag
     5461 gtattccgca catgatccgc tcgcttcttc gctccgccgc gccggcactc gtggccctgg
     5521 cccttgccgg atcggccaac gccgccgccg aaacgtggga catcgatacg agccactcga
     5581 acgtgggctt caccgtccgc cacaacgtca tcgcaaaggt ctatggccgc ttttcggagt
     5641 tcgagggaac cctggccctc gacccggcca acctgggggc cacggtggtt gatgtcacga
     5701 tccaggccgc cagcgtcgac accgacaacg agaagcggaa caaccacctc aagtcgcccg
     5761 actttttcga cgtggccaag taccccacca tcacgttcaa gagcacggcg gtaaccccca
     5821 aagacaagga aaccggcact ctcaagggcg acctgacgat caacggcgtc accaagccgg
     5881 tcgagctggc ctacaccgtg atcgggttca ccgacgcctg gggcggccag cgggccggtt
     5941 tcgaggccac gggctcgatc aatcgcaagg acttcaacgt caactggaac aagacgctcg
     6001 acaacggcgg cctggtggtc agcgacaagg tcgacatcac cctccagatc gaagccgtca
     6061 agaagaagcc ggaagaggcc aagccagccg gcaagtagca cgactctccc cgcaacggag
     6121 ttttcatgag cccgtcctcc cacccgggag ggcgggcttt ccgttgcgcc ccggcggttg
     6181 acatcgccgg aagctaaccg tactttccgt tggcacttgc gggctttttt gccccgaatt
     6241 ctccgtcccc gaccttgatc ctcccgagga ggccttcatg cgccgactgg ttaacgtttc
     6301 catccgtccg atccgattcg ccgcgctcct tatcctggcc ggactggccg tgtcgggctg
     6361 ccagggtggc ggcggtggca acgcgggtgg cggcaatggc ggcggaaaga cgagcggagg
     6421 cgcgccggcc ggcgcgccga tcaagatcgg t
//
LOCUS       DASVOD010000475         4676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_656505, whole genome shotgun sequence.
ACCESSION   DASVOD010000475 DASVOD010000000
VERSION     DASVOD010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656505"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VF720_10765"
     CDS             complement(<1..561)
                     /locus_tag="VF720_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX7879883.1"
                     /translation="MFRTYDEAVAWILSFVNWERVRSRKTMTVTTGDRTRFARALGRL
                     GHPERAVPAIHVGGTNGKGSTAAVTRALLTAHGLRTGLYTSPHLIDMRERVTIDGRPI
                     SRADFVKAAHQAARIVDAAPGSGFRTTFEILTALAFTAFRDASVDAMVIEVGLGGRLD
                     STNVLHPRAVVITSISLDHTEVLGETL"
     gene            627..1829
                     /locus_tag="VF720_10770"
     CDS             627..1829
                     /locus_tag="VF720_10770"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX7879884.1"
                     /translation="MNGQQPGSTFGRSIPAVIVAAVWLVFLIRGVHTLLIPPLAALAI
                     IFILYPYRQFSWAFRTMIVSGLIATLWLMAKVQTIVWIAAFGLFSAYVLNPVVMRLEK
                     VGIKRSRGALYLILVALTIVVAMAVVILPDLYSQAGDLVANLPRYLMEIQERYQGTLS
                     DPRYANLPIDLAGLADIASAKLAAMLSGADQGLASLLRNVGAALSIAILAPIVGYHLL
                     KDLPQIKSAALGLVPRNLEGELIDLITEMDVLIGRWLRGQFTVAMIIGTLTAIGLTIL
                     GIRYSIALGFLAGLFNMIPIVGYWASLILAVIIALTGNDPTHDALWTIGIFFAIQLLE
                     QNYISPRIVGRETGLHPVAILLSLLVFGALAGFAGALLAIPFTLFLRVAFRRYVARRL
                     RNARLPSA"
     gene            complement(1844..2617)
                     /locus_tag="VF720_10775"
     CDS             complement(1844..2617)
                     /locus_tag="VF720_10775"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX7879885.1"
                     /translation="MTRSGIVTLTTDFTTRGGYVGAMRGVILAHDRSLTIADITHDIE
                     PFNILEGALTLRAAARHFPTGTIHLAVVDPGVGGTRRAIIVEGSDGHFFVGPDNGLFT
                     PFFEGGRVSRIDPERAALANLSATFHGRDLFAPVAARLAKGTDPADFGPAIDDPVRLD
                     WPEPATEFDGWRGIVLTIDRFGNAITNLPQSLASPGRTLMTSGREAIPIVRTYAEASR
                     GTVVALVGSSGLIEVAVVEGSAGEVLELAVGSPVHLTKA"
     gene            complement(2614..2952)
                     /gene="cutA"
                     /locus_tag="VF720_10780"
     CDS             complement(2614..2952)
                     /gene="cutA"
                     /locus_tag="VF720_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX7879886.1"
                     /translation="MAEREAIVVLVSVPLDDTGDRLARKLVAEGLAACVHQMPAGRST
                     YRWQGAVEAAEEILLVIKTTRAAYPALEAVVRDAHPYDVPEILALAVEEGLPAYVKWL
                     VDSVTHGSKG"
     gene            complement(2968..4002)
                     /locus_tag="VF720_10785"
     CDS             complement(2968..4002)
                     /locus_tag="VF720_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7879887.1"
                     /translation="MTRRYQISSRALPALLLVGLLAPAAVRAETRVGGTLSGQVQWTK
                     AASPYVVTEDITIPVGSKLSIGPGVLVKFRPNLADQKGVRPFDLEIAVYGTLECSGAD
                     GDSVYLTSDSLDPSTQDWAGICVEKGGMARIDRMVLDSAATGISVTDGEIDLSRSTIR
                     ACSEKGLHFQRGKGRVYKSLFTMIGNFAGTAKGISLVASPDVLIDETFVIGAQIGIGL
                     ERNSDATIRKSLISLCRSYGIQITSSSPKVTENNIAQNEFGMLIRGRSEPVIKDNNIF
                     DNASWELQVRDYRANTPGEMPTLDLSGNWWGKITPDVAYDRIDDGNDNPSAGAAVKIE
                     PVRPQAWEAR"
     gene            complement(4073..>4676)
                     /locus_tag="VF720_10790"
     CDS             complement(4073..>4676)
                     /locus_tag="VF720_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879888.1"
                     /translation="RIIVAGAAARDTLEISANVSEGPRSQTPARVAGRPWPYQVPAPY
                     LTVVDRSDLTFRDRRFLNVSLAGLDGLRVISGPDSIQFEADSTGTWRLVGEPGTPGAP
                     PPDRTGLVEAWTRAVADSIVARGSPLDPFEARPAPRAPRLTLEAWDNDGRRLAGWEIL
                     AGPQDTYGARSILMDLPRPGEVLLVPSDLVLPFQAIFAGR"
BASE COUNT          726 a         1547 c         1613 g          790 t
ORIGIN      
        1 gagcgtttcg ccgagcacct cggtgtgatc gagcgagatg gaggtgatga cgactgcccg
       61 gggatgcagg acgttcgtgg aatcgaggcg cccccccaac cccacctcga tgaccatggc
      121 gtcgaccgac gcgtcgcgga atgcggtgaa ggccagcgcg gtcaggatct cgaacgtggt
      181 acggaagccg ctgccgggtg ccgcgtccac gatacgggcg gcctggtgcg cggctttcac
      241 gaagtcggcg cggctgatgg gccgcccgtc gatggtcacg cgctcccgca tgtcgatgag
      301 gtgcggcgag gtgtagaggc cggtgcgcag gccatgcgcc gtgagcaacg cccgggtgac
      361 agcggcggtg gagcccttcc cgttggtgcc gccgacgtgg attgcgggaa cggcccgttc
      421 ggggtggccg aggcggccga gggccctcgc gaagcgggtc cggtcgccgg tcgtgacggt
      481 catcgtcttc cgggagcgga cgcgctccca gttgacgaac gacaggatcc aggcgacggc
      541 ctcgtcatac gtgcggaaca tggcgcacga tgctagcagg ccgacgcccc ttatccgaag
      601 cccgcccgcc tgctagactg ccatgcgtga atggccagca gcccggatcc acgttcggac
      661 gatcgatacc ggccgtcatc gtcgcggccg tgtggctcgt cttcctgata cgcggcgtac
      721 acaccctcct catcccacca ctggccgcgc tggccatcat cttcatcctc tacccgtatc
      781 gacagttctc gtgggccttc cggaccatga tcgtgtcggg cctcattgca acgctctggc
      841 tgatggccaa ggtgcagacg atcgtctgga tcgccgcgtt cgggctcttc agcgcctatg
      901 tcctgaaccc ggtggtcatg cgtctcgaaa aagtggggat caagcggagc cgcggcgccc
      961 tctacctcat cctggtggcg ctcacgatcg tggtggccat ggccgtcgtc atcctccccg
     1021 acctctacag ccaggccggc gacctcgtgg ccaacctccc ccgctacctg atggaaatcc
     1081 aggagcgcta ccaggggacg ctgagtgacc cgcgctacgc caacctcccg atcgacctgg
     1141 ccggcctcgc cgacatcgcc tcggcaaaac tggccgccat gctgagcggc gccgaccagg
     1201 ggctggcctc gctcctccgg aacgtcgggg ccgcgctctc catcgccatc ctcgcgccga
     1261 tcgtcggcta ccacctgctg aaggacctcc cccagatcaa gtcggccgcc ctcggcctcg
     1321 tcccgcgcaa tctcgagggg gagctgatcg acctgatcac cgagatggac gtcctgatag
     1381 gccgctggct ccggggacag ttcaccgtgg ccatgatcat cggcacgctg accgccatcg
     1441 gcctgacgat cctcggcatc cgctactcga tcgccctcgg cttcctggcc ggcctcttca
     1501 acatgatccc catcgtcggc tactgggcca gcctgatcct ggccgtcatc atcgcgctga
     1561 ccggcaacga ccccacgcac gacgccctct ggacgatcgg catcttcttc gccatccagc
     1621 tcctcgagca gaactacatc tcgccgagga tcgtggggcg cgagacgggg ctgcaccccg
     1681 ttgcgattct tctctcgctg ctcgtgttcg gcgcgctggc gggattcgcg ggggcgctgc
     1741 tggccattcc gttcacgctc tttcttcgtg tggccttccg gcgttacgtc gcacgtcggc
     1801 tcaggaacgc gcggttgccg tcggcttagg gaaaaccggt cggctatgcc ttggtgaggt
     1861 gaaccgggct accgaccgcc agctccagca cctcccccgc actcccctcc accaccgcca
     1921 cctcgatcag tcccgacgat cccaccagcg ccacgacggt gccccgcgat gcctccgcgt
     1981 aggtccggac aatcgggatg gcctcacggc cactcgtcat cagggtgcgt ccgggggagg
     2041 caagagactg cgggaggttg gtgatcgcgt tcccgaaacg gtcgatggtg aggacgatgc
     2101 cgcgccatcc gtcgaattcg gtcgccggtt cgggccagtc gagacggaca gggtcgtcga
     2161 tcgcggggcc gaagtcggcg ggatcggtcc ctttggccag gcgtgcggca acgggtgcga
     2221 agaggtcgcg gccgtggaag gtggcggaga ggttcgccag tgccgcgcgc tccggatcga
     2281 tccggctgac ccgtcctccc tcgaagaacg gcgtgaacag tccgttgtcg gggccgacga
     2341 agaagtgccc gtcggagcct tcgacgatga tcgcccgacg tgtccccccg actccgggat
     2401 cgacgacggc caggtggatg gtgcccgtcg ggaagtgccg cgcggcggcg cggagcgtga
     2461 gggcgccttc gaggatgttg aagggctcga tgtcatgcgt aatgtccgcg atcgtgagcg
     2521 agcgatcgtg cgcgaggatc acgccgcgca tggcgcccac atagccgccg cgcgtcgtga
     2581 agtcggtcgt gagcgtgacg atgccggagc gggtcacccc ttgctcccgt gggtcaccga
     2641 gtcgaccagc cacttcacgt aggccggaag gccctcttcg accgcgaggg ccaggatttc
     2701 cggcacgtcg tacggatgtg cgtcgcgcac gacggcttcc agtgcgggat atgcggctcg
     2761 ggtcgtcttg atgacgagga ggatttcctc ggcggcttcg accgccccct gccagcgata
     2821 cgtggagcgt cccgcgggca tctggtgaac gcaggccgca aggccttccg caaccagttt
     2881 ccgtgcgagg cggtcaccgg tgtcgtcgag cggaacggag acgaggacga cgatcgcttc
     2941 ccgctcggcc atggcgcggg tgctggccta cctggcttcc caggcctgcg ggcggacggg
     3001 ctcgatcttg accgcggcgc cggcggaggg gttgtcgttg ccgtcgtcga tccggtcgta
     3061 ggccacgtcg ggcgtgatct tcccccacca gttgccggag aggtcgagcg tgggcatttc
     3121 gcccggggtg ttggcgcgat agtcccgcac ctggagctcc catgaggcgt tgtcgaagat
     3181 gttgttgtcc ttgatgaccg gctcggaccg gccacggatg agcatgccga attcgttctg
     3241 ggcgatgttg ttctcggtga ccttcggcga ggacgaggtg atctggattc cgtagctgcg
     3301 gcagagggag atgaggctct ttcgaatggt ggcgtccgag ttgcgctcga gcccgatgcc
     3361 gatctgggcg ccgatcacga aggtctcgtc gatcaggacg tcgggagatg ccaccaggct
     3421 gatccccttg gcggtgcccg cgaagttgcc gatcatggtg aagaggctct tgtacacccg
     3481 ccccttgccg cgctggaagt ggagcccttt ctcgctgcag gcgcggatgg tcgagcgcga
     3541 gaggtcgatt tcgccgtcgg tgacgctgat gccggtggcg gccgaatcga gcaccatgcg
     3601 gtcgatccgg gccatccccc ccttctcgac gcagatcccc gcccagtcct gtgtgctcgg
     3661 gtcgaggctg tccgaggtca ggtagaccga gtcaccgtcg gcgccggaac attccagcgt
     3721 gccgtagacg gcaatctcga gatcgaacgg acggacgcct ttctggtcgg ccaggttggg
     3781 ccggaacttg accagcacgc ccggaccgat ggagagcttt gacccgaccg ggatcgtgat
     3841 gtcctcggtg acgacataag gagaagcggc cttggtccac tggacctgtc ccgaaagcgt
     3901 gccccccacc cgcgtctcgg cgcgaacagc ggcgggggcg aggaggccga cgagcagcag
     3961 ggccggcagg gcccgggaag aaatctgata acgacgcgtc atgggtgacc tctgaaggga
     4021 atcgttggcc ggccatgggc cggcacgagc gcggtaaagt ttagcaggcg gcctaccgtc
     4081 cggcgaaaat ggcttgaaag ggcaggacga ggtccgacgg aacgaggagt acctctcccg
     4141 gccgggggag gtccatcaga atgcttcggg ctccgtaggt atcctgcggc ccggcaagga
     4201 tttcccaccc cgccaggcgg cgcccgtcgt tgtcccaggc ctcgagtgtg agccggggcg
     4261 cgcggggggc cggacgggcc tcgaatgggt cgagagggct ccccctggcc acgatgctgt
     4321 cggccacggc gcgcgtccag gcctccacca gcccggttcg atccgggggc ggggcacccg
     4381 gcgtgcccgg ctcgccgacc agccgccagg tgccggtcga atcggcttcg aactggatcg
     4441 agtcgggccc ggaaatgacg cgcaatccat cgagcccggc cagggagaca ttcaggaaac
     4501 ggcggtcgcg gaacgtgagg tcgctccggt cgaccaccgt caggtacggg gcgggcacct
     4561 gatagggcca gggacggccg gccacgcggg ccggcgtctg ggagcgcggc ccctccgaga
     4621 cgttcgcgga aatctcgagc gtgtcgcgcg ccgccgcacc ggccacgatg atgcgg
//
LOCUS       DASVOD010000476         6874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_292831, whole genome shotgun sequence.
ACCESSION   DASVOD010000476 DASVOD010000000
VERSION     DASVOD010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292831"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1315)
                     /locus_tag="VF720_10795"
     CDS             complement(206..1315)
                     /locus_tag="VF720_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879889.1"
                     /translation="MADSVRAATAALLIICSITACSDDPHDDWSRLGGADAPIYALQE
                     FRGRLVFGGEFTRVDAFGDDIEVHHLAAFADAENRALDPEVWDVLPHVTDAAGTWYNI
                     EPEPNGNVNAMTIFRGELIVAGAFTYVGPVRANGVARWNGFEWHALGDSVSGGGVQGT
                     VYALANDGDQVLYVGGNLSAAGGSAVRNVAQWNGAAWAPMDAGTNGTVYALHARARQL
                     FAGGAFSEAGGAPANHIASWTGLRWLPLDVGVDGTVRALTTWRGSLIAGGDFTTVNGF
                     EMNHVARWIGVGWLTMNGGLEAADAVTALAVNGEELWAGESRTVGSRLLDAWDGTSWE
                     GRLDGDGSALTGMSVGSYGVAASMETEGGAELWRR"
     gene            complement(1420..1698)
                     /locus_tag="VF720_10800"
     CDS             complement(1420..1698)
                     /locus_tag="VF720_10800"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX7879890.1"
                     /translation="MGIATGLGYSLFWDGFSQGQSLGKKLLGIRVIDFKTGAPCRYGQ
                     SFTRNSSSSLGAFDWAGVFTSSRRRPGDHSANTVVVRAVAPPAGPRAM"
     gene            2115..4469
                     /locus_tag="VF720_10805"
     CDS             2115..4469
                     /locus_tag="VF720_10805"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX7879891.1"
                     /translation="MALMLMLPGLALASELDLAIPELTTSYTLFGMTLSGTTLLMMGL
                     VVCVLGMLFGLMMFNQIKNMPAHQSMKDVSNIIYETCKTYLLQQGRLLMVLEALIAVC
                     IVYYFGFLEHKSFGTVGLILIWSVIGILGSYGVAWFGIRMNTYANSRTAFASLAGKPL
                     PVYEIPLKAGMSIGVLLICVELIMMLFILLFVPKESAGASFLGFAIGESLGASALRIA
                     GGIFTKIADIGSDLMKVVFKIKEDDPRNPGVIADCTGDNAGDSVGPTADGFETYGVTG
                     VALISFIALAVGGEHKAHLLVWIFVMRILMIATSIISYLVNDAVARSQNQGKAKIDFE
                     APLTSLVWLTSLLSIGVTYGVSYWMLADMGAGLWWKLSTIISCGTLAAALIPEFTKAF
                     TSSHSRHVKEIVAASREGGASLTILSGMVAGNFSVFWKGLTLAVLLLASYMISLMGLD
                     AFMTYPAVFAFGLVAFGLLGMGPVTIAVDSYGPVTDNAQSIFELSQVETTPGAAQEIE
                     KEFGFKPDFAKGKHFLEDNDGAGNTFKATAKPVLIATAVAGATTMVFSIILLLQSHYA
                     EAGHAFSLDLLDPRVLLGLIMGGATIYWFSGASMQAVTTGAYRAVEFIKKNIKIDSEA
                     KANIEDSKKVVQICTQYAQKGMFNIFMVIFCLTLAYAFFDPIFFVAYLVSIAIVGLFQ
                     AIYMANAGGAWDNAKKVVEVELGEKGTPLHDATVVGDTVGDPYKDTSSVAMNPIIKFT
                     TLFGLLAVEIGVANPTVAPFIGGALMLVALYFVYRSFYGMRITS"
     gene            5090..5872
                     /locus_tag="VF720_10810"
     CDS             5090..5872
                     /locus_tag="VF720_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7879892.1"
                     /translation="MRLVRSLVPLALLLVLSACGGSSSTPEKKDNTTATETPAVPLTT
                     VDPATAGTIEGTISFDGVPPADKVIDTKADPTCGRAHREPLYTETAVVADGKVRWAFV
                     RISGGLEGKSFGVPSEPVVVDQEGCVYVPHVVGVVTGQTIQVLNSDETLHNVNAVCAA
                     NKRFNMAMPLKGMKQEKKFATAEMVHFKCDVHPWMSAYIGVCDNPFYAVSEADGGYVI
                     RNVPPGDYTVEVWHETFGKLTMPVTVLSSGSARADFTFKAAS"
     gene            5875..>6874
                     /locus_tag="VF720_10815"
     CDS             5875..>6874
                     /locus_tag="VF720_10815"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX7879893.1"
                     /translation="MGGSAAASPRTGAWLRRYSRLVALATLFLIFAGGMVTSTGSGLA
                     VPDWPLSYGMLFPPMVGGIFYEHGHRMIAGGVGVLIAILAFWLLRSEPRRWVRRLGVA
                     AGLAVIAQALLGGLTVLLLLPDAVSISHAALAEIVFCLTVAIAVALSTGWRAAGESPI
                     ADVPEGSGGLARGAMLLTAIVYVQILLGAVMRHTGAGLAIPDFPLAYGRLIPASLAGG
                     IGIHFAHRVGALVVTLGVLAVVIHVVRLHRADGWLVRPVLALLALLVVQITLGALTIW
                     TIKAPVPTSLHVACGAATLAASLVVTLRTGRRAWVAERMTARGATTAGAPSITNADT"
BASE COUNT         1141 a         2388 c         2138 g         1207 t
ORIGIN      
        1 gtagatggcg ctcccccgcg catgaactcg gtgcgaatgg gactgccaac caacccgcga
       61 tcaggcaatc aagaggaata ctcttcgggc gctccgcgac gcggaaccag caccacccca
      121 acctccccag cagagcgagc catctccggg ggattcatcc gaatgctctt ccgcatcctc
      181 ctcctccccc tccgccccca ctaatctacc gcctccacaa ctccgcaccc ccctccgtct
      241 ccatcgaggc ggcaacacca taactaccaa ccgacatccc ggtcagcgca gagccgtcac
      301 cgtcgagtcg cccttcccac gacgtgccat cccaggcgtc gagtagtcgg ctaccgacgg
      361 tgcggctctc gccggcccag agctcctcgc cgttcacggc gagggcggtc acggcatcgg
      421 ccgcctcgag acctccgttc atcgtcagcc atcccacgcc gatccatcgc gccacgtggt
      481 tcatctcaaa gccgttcacc gtcgtgaagt ccccgccggc aatcagcgag ccgcgccagg
      541 tcgtcagcgc ccgcacggtg ccatccaccc ccacatcgag cggcagccac cgcagccccg
      601 tccaggacgc aatgtgattg gccggcgcac cgccggcctc cgaaaacgcg ccgccggcaa
      661 acagctggcg cgcccgcgca tgcagggcgt acaccgtgcc gttggttccg gcatccatcg
      721 gtgcccaggc ggcgccgttc cactgggcca cgttccgtac cgcggatccg ccggcggcgg
      781 aaaggttgcc gcccacgtag agcacctggt cgccatcatt ggccagcgcg tacaccgtgc
      841 cctgaacgcc gccgccggaa accgaatcgc ccagcgcgtg ccactcgaaa ccattccatc
      901 gcgccacgcc gttggcccgc accggcccca cgtacgtgaa ggccccggcc acgatcagct
      961 cgccgcggaa aatcgtcatg gcgttcacgt tgccgttggg ctcgggttcg atgttgtacc
     1021 aggtgccggc ggcgtccgtg acatgcggca gcacatccca cacctccggg tcgagcgcgc
     1081 ggttttcggc atcggcgaag gcggcgaggt ggtgtacctc gatgtcgtcg ccgaacgcgt
     1141 ccacccgggt gaactcgccg ccgaagacga ggcgcccgcg gaattcctgg agggcgtaga
     1201 tgggggcgtc ggcgcccccc aggcgggacc agtcgtcgtg agggtcgtcg gagcaggcgg
     1261 tgatgctaca tataatgagg agggcggccg tggccgccct taccgaatca gccaactcat
     1321 tgaaatacaa agatttccgt acagatgcgc cgatggcaag cggatgccgg tcgtgggatc
     1381 tcgttgtcac gccgtcaatt atacgctgtc ccaaccccgc tacatggcgc ggggccctgc
     1441 gggaggcgcc acggcccgca ccaccaccgt attcgccgag tggtcgccgg gccggcgccg
     1501 actggaagtg aagactccgg cccagtcgaa ggcgccgaga gatgacgacg aattccgggt
     1561 gaacgactga ccgtaccggc acggcgcgcc cgtcttgaag tcgatcacgc ggatgccaag
     1621 gagtttcttg ccgaggctct gtccctggct gaatccgtcc cagaagaggg agtaacccag
     1681 acccgttgcg atccccaccc acgccacgta tccgcccggt ttgccgaatc gtgatccgag
     1741 caggtaaccg gcccacgtaa ctgttttgaa tacagttact tcaccgatag cggcagtcat
     1801 tcgggcggtg gaacggccac gatcgggcag cggcagggac ccaatggtga gccatccccc
     1861 accccgaaat tacttgccgc ctcgcacccc tctcggctag actcccccgc tcttgcccga
     1921 cgcgtaactg tggacggcaa ttgaagttcc aacctcatcg caaccgggca aagctccgtg
     1981 aggacgtctc gctcgccggt tgcggcccaa ctcgacttcg acaatcgcga acaaccacta
     2041 atgaggagta tctgatgacg atgctgcgga cactggccgc gcgcctcgcc ggggcaaagc
     2101 tgcccctggt gctgatggcg ctgatgctca tgttgccggg gttggccctg gccagcgagc
     2161 tcgacctggc gatcccggaa ctgacgacgt cgtacaccct gttcgggatg acgttgagcg
     2221 gcaccaccct cctcatgatg ggcctggtcg tctgcgtcct ggggatgctg ttcggcctca
     2281 tgatgttcaa ccagatcaag aacatgccgg cgcaccagtc gatgaaggac gtgtcgaaca
     2341 tcatctacga gacgtgcaag acgtacctcc tccagcaggg gcgcctcctg atggtgctgg
     2401 aggccctcat cgccgtctgc atcgtctatt acttcggctt cctcgagcac aagtcgttcg
     2461 gcacggtggg cctcatcctc atctggtcgg tcatcggcat cctcgggtcg tacggcgtgg
     2521 cgtggttcgg catccgcatg aacacctacg ccaacagccg caccgccttc gccagcctcg
     2581 ccggcaagcc gctgccggtg tatgaaatcc cgctgaaggc cggcatgtcg atcggcgtgc
     2641 tgctcatctg cgtcgagctc atcatgatgc tcttcatcct gctcttcgtg cccaaggagt
     2701 cggccggcgc cagcttcctc ggctttgcca tcggcgagtc gctcggcgcc tcggcgctcc
     2761 gtatcgccgg cggcatcttc accaagatcg ccgacatcgg ctccgacctc atgaaggtcg
     2821 tgttcaagat caaggaagac gatccgcgca accccggcgt catcgccgac tgcaccggcg
     2881 acaacgccgg cgactcggtg ggccccacgg ccgacggctt cgagacctac ggcgtgacgg
     2941 gcgtggcgct catcagcttc atcgcgctgg cggtgggcgg cgagcacaag gcgcacctgc
     3001 tggtgtggat cttcgtgatg cgcatcctga tgatcgccac ctcgatcatc agctacctcg
     3061 tcaacgacgc ggtggcccgc agccagaacc aggggaaggc caagatcgac ttcgaggccc
     3121 cgctgaccag cctcgtgtgg cttacctcgc tcctctcgat cggcgtgacg tacggcgtgt
     3181 cgtactggat gctggccgac atgggcgcgg gcctctggtg gaagctgtcg acgatcatct
     3241 cgtgcggcac gctcgccgcg gcgctgatcc cggagttcac caaggcgttt acctccagcc
     3301 acagccggca cgtgaaggaa atcgtggcgg ccagccgtga gggcggtgcc tcgctcacga
     3361 tcctctcggg catggtggcc ggcaatttct ccgtgttctg gaaggggctg acgctggcgg
     3421 tgctgctgct ggccagctac atgatctccc tcatgggcct cgacgccttc atgacgtatc
     3481 cggccgtgtt cgccttcggc ctggtggcgt tcggcctcct gggaatgggc ccggtaacga
     3541 tcgccgtgga cagctacggc cctgtgaccg acaacgccca gtcgatcttc gagctcagcc
     3601 aggtggagac gaccccgggc gccgcgcagg agatcgagaa ggaattcggc ttcaagcccg
     3661 actttgccaa ggggaagcac ttcctcgagg acaatgacgg cgcgggcaac acgttcaagg
     3721 ccaccgccaa gccggtgctc atcgccacgg ccgtggccgg cgccacgacg atggtcttct
     3781 cgatcatcct cctgctccag agccactacg ccgaggccgg ccacgccttc agcctcgacc
     3841 tcctggatcc gcgtgtcctc ctgggactga tcatgggagg cgccaccatc tattggttct
     3901 ccggtgcctc catgcaggcc gtgaccaccg gcgcctaccg cgccgtcgag ttcatcaaga
     3961 agaacatcaa gatcgactcc gaagccaagg ccaacatcga ggactccaag aaggtggtcc
     4021 agatctgcac ccagtatgcg cagaagggga tgttcaacat cttcatggtc atcttctgcc
     4081 tgacgctcgc gtacgccttc ttcgacccca tcttcttcgt ggcctacctg gtttcgatcg
     4141 ccatcgtcgg cctcttccag gccatctaca tggccaacgc cggcggcgcc tgggacaacg
     4201 ccaagaaggt ggtggaggta gagctcggcg aaaagggaac gccgctgcac gacgccacgg
     4261 tcgtcggcga caccgtcggc gacccctaca aggacaccag cagcgtggcc atgaacccca
     4321 tcatcaagtt caccaccctg ttcggcctgc tggcggtcga gatcggcgtc gccaacccca
     4381 cagtggcccc cttcatcggc ggcgccctca tgctggtagc cctgtacttc gtctaccgct
     4441 ccttctacgg catgcgcatc accagctagt tcttgtcttg aggggcccca gcgccccgcg
     4501 aagcggcagc gccctcgagc aagccgcgca gcggcaaagc gagggcaagg cctcgattca
     4561 agcaacaaaa gacccctgca aggtcgtgag accgcagggg tcttttcttt tgctcttcaa
     4621 aggggcaagg ccccgcgttt gtacgccgcc gtgccacccg gggtggtaga caggggtggg
     4681 ggcaaagcgc gcgcgccccg gcgcatgtcc ttctaagcaa ggggtgcgcg cgctttgtcg
     4741 ccacccctga ccgggagcca aaagagtggc cccggccggc ataaaaaccg accggggccc
     4801 tttggggctc cccgcaacgg gagtcgtcgc gatctttccg gggctggggg cgaagaacac
     4861 tggaacactg gagtcgcgcc gcccggcacg tgagtatcgt gcgtccgtcc aatcccgctg
     4921 acaaggggga aaatacggtt cggccgacaa atttttcggc cgcctgaatc gctcgtcgcg
     4981 gtcccaaaaa cccggttgaa atgcccggat gtcggaccta ccatttcagg ctgtttgcgt
     5041 tggattcccg cggcgaggtc gccgcgcccc tgtcgaaagg ttccgcttca tgcgcctcgt
     5101 tcgttcgctc gttcccctcg cgttgcttct cgtgttgtcc gcctgcggcg gttcgtcgtc
     5161 gacacccgaa aagaaggaca acaccaccgc aacggagacg ccggcggtgc cgctgaccac
     5221 cgttgatccc gccaccgccg gcacgattga gggaaccatc tcgttcgacg gcgtgccgcc
     5281 ggccgacaag gtgatcgaca ccaaggccga cccgacctgc ggcagggcgc atcgcgagcc
     5341 tctctacacg gaaacggccg tcgtggccga cggcaaggtg cgctgggcct tcgtccggat
     5401 ttcgggcggc ctcgaaggaa agagcttcgg cgtgccgtcc gagccggtgg tggtggacca
     5461 ggaggggtgc gtgtacgtgc cgcacgtggt gggtgtcgtg accggccaga ccatccaggt
     5521 gctcaactcc gacgagacgc tgcacaacgt caacgcggtg tgcgccgcca acaagcgctt
     5581 caacatggcc atgccgctca agggaatgaa gcaggagaag aagttcgcca cggccgagat
     5641 ggtgcatttc aagtgcgatg tgcacccctg gatgtcggcg tacatcggcg tgtgcgacaa
     5701 ccctttctac gccgtcagcg aagcggatgg cggctatgtt attcgcaacg tgccccccgg
     5761 ggactacacg gtcgaggtct ggcacgagac cttcggcaag ctcaccatgc cggtcaccgt
     5821 gctttcctcg ggcagcgccc gggccgactt caccttcaag gccgcgtctt gaccatgggc
     5881 gggagcgcgg ccgcaagccc ccggacgggg gcctggctcc ggcgttactc ccgcctcgtg
     5941 gcgctcgcca cgctctttct gatctttgcc ggcggcatgg tcacctccac gggctccggc
     6001 ctggccgttc ccgactggcc cctctcgtac ggcatgctct ttcccccgat ggtcggtggc
     6061 atcttctacg agcatggcca ccggatgatc gccggcggcg tgggggtcct catcgctatc
     6121 ctggctttct ggctccttcg gtcggagccc cggcgctggg ttcgccgact cggcgtggcg
     6181 gcgggccttg cggtcatcgc ccaggcgctg ctcggcggcc tcaccgtgct cctcctcctg
     6241 ccggacgccg tgtcgatcag ccacgccgcg ctggccgaga tcgtcttctg cctgacggtg
     6301 gcgatcgcgg tggcgctctc gaccggatgg cgcgcggcgg gtgagtcgcc gattgccgac
     6361 gttccggaag gatcgggcgg gctcgcgcgc ggagccatgc tgctcaccgc catcgtgtac
     6421 gtgcagatcc ttcttggcgc cgtgatgcgc cacaccgggg ccggcctcgc catccccgat
     6481 tttccgctgg cttacgggcg gttgatcccg gcatcgctgg ccggcggcat cggcattcat
     6541 ttcgcgcacc gggtcggtgc gctcgtcgtt accctgggcg ttctggccgt ggtgatccac
     6601 gtcgtgcgcc tccaccgggc ggatggctgg ctcgtgcggc cggtcctggc gctcctggcg
     6661 ctcctggtcg tgcagatcac gctcggggcg ctcaccatct ggacgatcaa ggccccggtg
     6721 cccacgagcc tccacgtggc gtgcggcgcg gccacgctgg cggcgagcct ggtggtgacg
     6781 ctgcggaccg ggcgtcgcgc ctgggtggcg gagcggatga cggcgcgggg ggcgaccacc
     6841 gccggggccc cgtcgattac gaatgccgac accc
//
LOCUS       DASVOD010000477         3167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_292877, whole genome shotgun sequence.
ACCESSION   DASVOD010000477 DASVOD010000000
VERSION     DASVOD010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292877"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /locus_tag="VF720_10820"
     CDS             complement(<1..1195)
                     /locus_tag="VF720_10820"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7879894.1"
                     /translation="MRRPARARRHESILCAAIAFAVLVAGCSNAPRPTPSPQVTDVNA
                     FLDDLEERTFRWFWDVTDSTNGLTPDRWPTPSFHSIAATGFALTAYPIGVERGWVSRE
                     AAARRTATTLRFLWTAPQDSVASGVIGYKGLFYHFLEPEHGTRFKDVELSTVDTALLF
                     AGVLTCQSWFDGNTPDEITIRALADSLYLRADWNWASPRAPLIGHGWTPEEGHLKWDW
                     RGYNEAMLVYIIALGSPTHAPAPAAWDSWLDGYTWGTFEGQAHVGFAPLFGHHYSHVW
                     VDFRGIRDSYMQTKGIDYFENSRRATLSQRSYAIRNPMAFEGYGPDIWGLSACDGPVN
                     GTVTIKGKPVEMATYRARGVSYGDIRDDGTIAPTAAGGSIPFAPEFAIPALMAMKSAW
                     GEHGYN"
     gene            1268..2239
                     /locus_tag="VF720_10825"
     CDS             1268..2239
                     /locus_tag="VF720_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7879895.1"
                     /translation="MQRRTAWRFIRLLAWIIVVILLFRARAGVDWRHLFGRAPTTGAA
                     ANTVILGGDDLAPRLIDRLLIPFCRDHPGLLVTRQGGGTNRALERVINREADLAVTAR
                     APTPAEQELAREATGDTLVVHRLALGALVVWKGDTDHETAPAGEAALTLANLRHLALE
                     SAATGGAHLVAPDPNDGTWSAFLAVVAPGVADTFKAPDVQFVADAAGVLAAVRVDPKA
                     LGITGWMAAPEPAPGDDVQAVPITGGGPQSLPVGPTYETIGTGTYPLIHSLHVVARPG
                     GGAAATMLLTYLVGDDGQRQIERAGFLPARRALRTVVLSTDPIGETH"
     gene            2257..>3167
                     /locus_tag="VF720_10830"
     CDS             2257..>3167
                     /locus_tag="VF720_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879896.1"
                     /translation="MTNRRATRVIVFLLMSFVLPVMAGSPDLLLPGAALAEPSYPMDT
                     DPIRLGRKAADQGNWDEAHRRFDEAMTAGYHVDEAEEGLARVAVVEGRLADAEALYRR
                     AIEDHAKKDGEFPEARAGLGLLLLRLDRVPEAAAEFDLALAADDDCWPAQYGRGRVLL
                     LTNGDPAEARRLIENGAKKKTPAAHEAMYHHGMGLVHLSADRLADAEAEALIAAGLDA
                     GNPEYARLVAEIYEKKNIPSLAATTYERLLTLPGAAPLAPVHRALGRSYERMQRWNDA
                     RDQYVKAVQADSTLAPAWLDLGNLLHRA"
BASE COUNT          508 a         1065 c         1117 g          477 t
ORIGIN      
        1 ggttgtagcc atgctctccc caggccgact tcatggccat gagtgccggg atggcgaact
       61 ccggcgcgaa cgggatcgag ccgccggccg ccgtgggggc gatcgtgccg tcgtcgcgga
      121 tgtcgccgta cgaaacgccg cgcgcccggt aggtggccat ctcgaccggc ttccccttga
      181 tcgtgaccgt gccgttcacc ggcccatcgc atgccgagag gccccagatg tcgggaccgt
      241 acccctcgaa cgccatggga ttgcgaatgg cgtacgaccg ctgcgacagc gtggcgcgcc
      301 gcgagttttc gaagtagtcg attcccttcg tctgcatgta cgaatcgcgg atcccgcgga
      361 agtcgaccca gacgtggctg tagtgatggc cgaagagggg tgcgaagccg acgtgcgcct
      421 gcccctcgaa cgtgccccag gtgtaaccgt cgagccagga atcccaggcc gccggcgccg
      481 gtgcgtgcgt gggcgagccg agggcgatga tgtagacgag catcgcctcg ttgtagccgc
      541 gccagtccca cttgaggtgc ccttcctcgg gagtccatcc atggccgatg agtggcgcgc
      601 gcggcgaggc ccagttccag tcggcccgca ggtagagcga atcggccagc gcgcggatcg
      661 tgatttcgtc cggcgtgttc ccgtcgaacc atgactggca ggtgaggacg cccgcgaaga
      721 ggagggcggt gtcgaccgtc gagagctcca cgtccttgaa acgggtgcca tgctccggct
      781 cgaggaaatg gtagaagagc cccttgtagc cgatgacgcc cgaggccacc gagtcctgcg
      841 gcgcggtcca gaggaagcgg agcgtggtgg ccgttcgccg ggcggcggcc tcgcgagaga
      901 cccacccccg ctcgaccccg atcgggtagg cggtgagcgc gaagccggtg gcggcaatgc
      961 tgtggaacga tggcgtgggc cagcggtcgg gcgtcaagcc gttggtcgag tcggtcacgt
     1021 cccagaacca gcggaaggtg cgctcttcca ggtcgtccag gaaagcgttt acatcggtga
     1081 cctgcgggga cggggtgggg cggggcgcat tggagcaacc ggcaacgagg acggcgaaag
     1141 caatcgcggc acacagaatg gattcgtggc gccgggcgcg ggcggggcgt cgcattcggg
     1201 tattgtcatc gcccgggcgt cccttggcaa ccgaggcgac ccgaaaaggg acgggggagg
     1261 aaagaggatg cagcggcgga cggcgtggcg gttcatccgc ctcctggcct ggatcatcgt
     1321 ggtgattctt ctcttccgcg cgcgcgcggg agtggactgg cgccacctgt tcggacgcgc
     1381 cccgacgacg ggcgccgcgg ccaataccgt catcctcggc ggcgacgacc tggcgccgcg
     1441 actcatcgac cgcctgctca tccccttctg ccgtgatcac cccggcctcc tggtcacgcg
     1501 ccagggaggg ggcaccaacc gggcactcga gcgggtcatc aaccgggagg ccgacctggc
     1561 ggtgacggca cgcgcaccga caccggccga gcaggaactg gcacgcgagg ccaccggaga
     1621 tacgctggtc gttcaccggc tggcgctggg ggcgttggtg gtgtggaagg gtgatacgga
     1681 tcacgaaacc gcccctgccg gcgaggccgc actcaccctg gccaacctcc gtcacctggc
     1741 actcgaaagc gcggcaaccg gcggcgccca tctcgtggcc ccggatccga acgacggcac
     1801 ctggagtgcg ttcctggccg tggtggcgcc cggggtggcc gacacgttca aggccccgga
     1861 cgtccagttc gtggccgatg ccgcgggggt cctcgcggcc gtgcgagtcg atccaaaggc
     1921 cctgggcatc accggttgga tggccgcgcc ggaaccggcg cccggcgacg acgttcaggc
     1981 cgtgccgatc accggcggcg ggccccaatc cctcccggtc ggccccacat atgagaccat
     2041 cggtacgggg acttaccccc tcatccactc cctccacgtc gtcgcgcggc cgggcggcgg
     2101 cgcggcggct acgatgctcc tgacttatct cgtcggcgac gacgggcagc ggcagatcga
     2161 gcgcgccggg ttcttgccgg cccgccgcgc cctgcgaacc gtggtcctgt cgaccgaccc
     2221 gatcggagag actcactgac attctctctc gatgacatga cgaatcgtcg cgcgacgcgc
     2281 gtcattgttt tcctgctgat gtcgttcgtc ctgcctgtca tggcaggatc gcccgatctc
     2341 ctcctgccgg gtgccgccct cgccgaaccg agctacccca tggacaccga ccccatccgg
     2401 ctcggccgca aggccgccga ccaggggaac tgggacgaag cacaccgccg cttcgacgag
     2461 gcgatgacgg ccggctatca cgtggatgaa gccgaggaag gactcgcccg cgtggccgtc
     2521 gtcgaggggc gactggccga cgccgaagcg ctctatcgac gtgccatcga ggatcatgcg
     2581 aagaaggacg gcgaattccc cgaggcccgg gcgggcctgg gacttctgct cctccggctt
     2641 gatcgggtgc cggaggcggc ggccgagttc gacctggcgc tggcggcgga tgacgattgc
     2701 tggccggccc agtacggtcg tgggcgtgtc ctcctcctca cgaatggtga tcctgccgaa
     2761 gcccgccgcc tgatcgaaaa cggagcgaag aagaagacac ccgccgcgca cgaagccatg
     2821 taccaccacg gcatggggct cgtgcacctg tcggccgaca ggctggccga cgcggaggcc
     2881 gaggcgctca tcgccgccgg tctggatgcg ggaaacccgg agtacgcgcg cctggtggcg
     2941 gaaatctacg agaagaagaa catcccctcc ctggccgcca cgacgtacga gcggcttctc
     3001 acgctgccgg gtgcggcgcc gctcgcgccc gtgcatcgtg ccctcggccg gagctacgag
     3061 cggatgcagc gctggaacga cgcgcgcgat cagtacgtga aggccgtgca ggccgattcg
     3121 acactggcgc cggcctggct cgaccttggc aatctcctgc atcgtgc
//
LOCUS       DASVOD010000478         6065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_449675, whole genome shotgun sequence.
ACCESSION   DASVOD010000478 DASVOD010000000
VERSION     DASVOD010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449675"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VF720_10835"
     CDS             <1..193
                     /locus_tag="VF720_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879897.1"
                     /translation="VANLPTPWGPITWSMRRSRNTDPIDVTIEGLKSLPPQGIVVRAP
                     GCADEVVTALPARLSLSPR"
     gene            complement(153..1556)
                     /locus_tag="VF720_10840"
     CDS             complement(153..1556)
                     /locus_tag="VF720_10840"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018972343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX7879898.1"
                     /translation="MSRPAGLATGTNLFPADFVWGAATSAYQIEGSPLADGAGPSNWH
                     RFCHTPGMTERGATGDVASDHYRRWPEDVALMKWLGLGAYRFSLSWSRILPGGYGRLN
                     ETGLDFYDRLVDALLAAGIDPWITLFHWDLPAALDERGGWTNPDIAGWFGDYAAVVYA
                     RLGDRVRRWMTLNEPWVVMDAGYLHGVHAPGHRSKFEAARAAHQLMRAHGAGVAAFRA
                     AGAPGEIGLVVNLEPKYPETRSAKDVAAARRADAYMNRWFLDGARFGSYPEELPGIFA
                     EAWPEIDPADFGPMTAPIDFVGVNYYTRGIVRNDPKGRPFDWYRVPPKKRGATETGWE
                     VHAPSFTRILEWVKERYGDLPIYVTENGSAFPDPPVARGAALADPKRVAYLQDHLSAA
                     ADAIAAGVNLRGYFAWSLLDNFEWGSYEKRFGLIHVNYRTQKRTPKASAHVYRNVIAT
                     NGALLSAAKAKGARGAR"
     gene            complement(1553..5416)
                     /locus_tag="VF720_10845"
     CDS             complement(1553..5416)
                     /locus_tag="VF720_10845"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEX7879899.1"
                     /translation="MIPPAAPPFGPKTAPAFAGRFLSNGRYHLLMTPFATGYSQWGTL
                     RLSAWSADRTADAEGSHVWLQEPADGTAWRLGGTDAPASASYRSDGRIDLAGEKNDIE
                     WRLAVAVLPGADAELRRLVLRNRSDHPRNFVVRTVTDVVLHEAQAHASHPTFSRLFLQ
                     TDVEPGLRLLLAARRPRSPEESYPAMFQVLHGGSGWRWETDRARLVGRRLPGELPRFA
                     MAEDNAPGTAGSVLDPVLSQAARVTLAPGEARTIDILVGVTAEREEARRLAEQAAVPA
                     QVAAWIDEAREMEEARRMGLECDRADADYFEAVAGAILAGVPSLGDLSAPVPGPHINL
                     APFALDGRCHLALHDPASELPEEASHARGFDRMARYWNALGLPIQSIRVRATSGTEDG
                     AVPRDITATEDERMALRHRAALVLREGLPTLAYGVMETDRSPAATPSARRDVTSASDE
                     VATSVGQSRGTLTFDNGHGGFAADGREYVIRMERRSDGTFRRTPLPWTNVLANENFGC
                     IVSETGAGVTWSRNSREYRLTPWSNDPLLDVPGECFYVRDEATGRFGSPLPGPAPWSA
                     DYEMRHAPGVSRAHVTADGLDMSTDIFVVRHDPVRVTRIRITERNGTARRLSVYAAAR
                     LVLGHAPEDSGRFVTTAAGPSAGSLTATTRRGGDVTGRVAVAGVVTTDARAELSIGGD
                     RATFQGVHGTLAAPRAVVSGEPLDGRTGPGLDPMFGQRAVIDLPAGGTVEVAFLLGDA
                     ASHAEARGLLERYQDAADIEAARKTSDRWWREELGGLSIETPSPALDLMVNHWLPYQT
                     VACRLWGRSAFYQSGGAFGFRDQLQDSMSLLLLRPAMAREQLLRNAAHQFPEGDVLHW
                     WHPPLGAGIRTRFADDRLWLPLFAAEYVAATGDESLLDEMVPWVTARALEAGEDEAYL
                     RSTTTDRADTLYEHCLRAIEISLETGAHGLPLFGTGDWNDGMNRVGREGRGESVWMGF
                     FLYKVLGDFLPYCERKGDEMRVQRYRLHRERLREALEQGGWDGAWYRRGYYDNGTPLG
                     SCEGEECRIDALAQAWAVLSGAASPERARQAMENCRSWLVSEDEGLIRLLTPPFDRTI
                     EDPGYIKGYVAGVRENGGQYTHAALWVVRAMAELGRNDLAARWLDLLNPVHHSATAAD
                     VERYRVEPYVVAADVYGAPPHVGRGGWTWYTGSAGWMYRVAVESILGLEWRDGKHLVL
                     RPCLPDDWKRVTIRWRPPGGLTRYTITLMNPRGAGLGVGAATIDGVAIPIVDGAAHLD
                     LATDGLEHHVLLETGATEAAPVPAS"
     gene            5460..>6065
                     /locus_tag="VF720_10850"
     CDS             5460..>6065
                     /locus_tag="VF720_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEX7879900.1"
                     /translation="MKTRFGQTSVIFFPGRKSNRGSIVTTIRDVARASGVSIATVSRV
                     INDSARVNDDTRRRVWDAAMRLDFWPNGAARTLTTSRTHAIGVLLPELHGEFFSEVIR
                     GIDRAARRERLQLLISSSHADTEAMLAAARTMRGRVDGLIVMSPEKGSQAAIEQIRRR
                     FPMVLLNSSEPAPDGRSITVANLVGAYQVARHVLDGNHRHVA"
BASE COUNT          943 a         2159 c         2039 g          924 t
ORIGIN      
        1 cgtggcaaat ctgccgacac cctggggccc gatcacctgg tcgatgcggc gctcgcggaa
       61 caccgacccg atcgacgtca cgatcgaggg actgaagagc ctgccgcccc aggggatcgt
      121 ggtgcgggca cccggctgcg cggacgaagt ggttaccgcg ctccccgcgc gcctttcgct
      181 ttcgccgcgc tgagcagggc cccgtttgtg gcgatcacat tgcggtagac gtgggcgctc
      241 gccttgggcg tccgcttctg cgtccggtag ttcacgtgga tcaagccgaa gcgcttctcg
      301 tagctccccc actcgaagtt gtcgaggagt gaccaggcaa aatagccgcg caggttgacg
      361 cccgcagcga tggcgtcggc cgccgccgaa aggtgatcct gcagataagc cacccgtttc
      421 ggatcggcca gcgcggcgcc ccgcgccacg ggcggatcgg gaaaggcaga gccgttctcg
      481 gtcacgtaga tcggcaggtc gccgtagcgc tccttcaccc attcgaggat gcgcgtgaag
      541 ctcggcgcgt gcacttccca gccggtttcg gtggcgcccc gcttcttcgg cggcacgcga
      601 taccagtcga acggtcgccc cttcggatcg ttccgcacga tgccccgcgt gtagtaattc
      661 acgcccacga agtcgatcgg cgccgtcatc ggcccgaagt cggcggggtc gatctccggc
      721 cacgcctcag cgaagatgcc ggggagttcc tcgggatagg aaccgaaacg agcgccgtcc
      781 aggaaccagc ggttcatgta ggcatcggct cgacgggcgg ccgccacatc cttcgcactc
      841 cttgtctcgg gatacttggg ctcgaggttc acgacaaggc cgatctcccc gggtgcgccc
      901 gccgcgcgga aggccgccac gccggcccca tgggcccgca tcaactggtg ggcggcccgg
      961 gccgcctcga acttgctgcg atgtccgggg gcatgcacgc cgtgcaggta gccggcgtcc
     1021 atcacgaccc acggttcgtt cagcgtcatc cagcggcgaa cccgatctcc cagacgggcg
     1081 tagacgacgg cggcgtagtc cccgaaccag ccggcgatgt cgggattcgt ccagccgccc
     1141 cgctcgtcga gcgcggccgg gaggtcccag tgaaacaggg tgatccaggg atcgatcccc
     1201 gcggcaagga gcgcgtcgac cagccgatca tagaaatcga ggcccgtttc gttcaggcgg
     1261 ccatatcccc cgggcaggat gcggctccac gagaggctga agcggtacgc gccgaggccg
     1321 agccacttca tgagggccac gtcttccggc cagcggcggt agtggtccga ggccacgtcg
     1381 ccggtggctc cccgctccgt catccccggt gtgtggcaga agcggtgcca gttgctgggc
     1441 cccgcgccat cggccagggg cgaaccctcg atctggtagg ccgaggtggc ggcaccccac
     1501 acgaaatcgg cgggaaagag gttggttccc gttgcgaggc cggctggtcg gctcacgagg
     1561 ccgggacggg ggcggcttcg gtggccccgg tttcgaggag cacgtggtgc tcgaggccgt
     1621 cggtcgcgag gtcgagatgt gccgcgccgt ccacgatcgg gatcgccacc ccgtcgatgg
     1681 tagccgcgcc aacgccgagc ccggcaccgc gcggattcat gagggtgatc gtgtagcgcg
     1741 tgagcccgcc cggcggacgc cagcggatcg tcaccctctt ccagtcatcg gggaggcacg
     1801 gacgcaggac gaggtgcttt ccatcgcgcc actcgagtcc caggatcgac tcgaccgcca
     1861 cccggtacat ccatcccgcc gagccggtgt accaggtcca tccgccgcgt ccaacgtgag
     1921 gtggtgcgcc atatacgtcc gccgccacga cgtacggttc cacgcggtaa cgctccacgt
     1981 cggccgccgt ggccgagtgg tgcaccggat tcaggagatc gagccagcgc gcggccaggt
     2041 cgttgcgccc cagctcggcc atggcccgca cgacccagag ggccgcgtgc gtgtactggc
     2101 cgccgttctc acgcacaccc gccacgtaac ccttgatgta gcccggatcc tcgatcgtcc
     2161 ggtcgaaggg aggggtgagg agccggatca ggccttcgtc ttccgacacg agccacgaac
     2221 ggcaattctc catcgcctgc cgggcacgtt cgggcgaggc cgcgcccgac agaaccgccc
     2281 acgcctgggc cagcgcgtcg atgcggcact cctccccttc gcacgatccg agcggcgtgc
     2341 cgttgtcgta gtagccgcgc cggtaccacg cgccgtccca gccaccctgc tcgagcgcct
     2401 cgcgcagccg ttcgcgatgc agccggtagc gctgcacgcg catttcgtcc cccttgcgct
     2461 cgcagtacgg gaggaaatcg cccagcacct tgtagaggaa gaagcccatc cagacgctct
     2521 cccccctccc ctcgcgcccg acccggttca tcccgtcgtt ccagtccccg gtgccgaaga
     2581 gcggaaggcc gtgcgcgccc gtctcgagcg agatctcgat ggcccgcagg cagtgttcat
     2641 atagcgtgtc cgcacgatcg gtggtggtgg acctgaggta ggcctcgtcc tcccctgctt
     2701 cgagcgcacg cgccgtgacc cacggcacca tctcgtcgag cagtgactca tccccggtag
     2761 ccgccacgta ctcggccgcg aagagcggga gccacagccg atcatcggcg aagcgcgtgc
     2821 gaatgccggc accgagcggc ggatgccacc agtggagcac gtccccctcg gggaactggt
     2881 gggcggcgtt ccgcaggagc tgctcgcggg ccatcgccgg acgcagcagc agaagggaca
     2941 tcgagtcctg cagctggtcg cggaagccga aagcgccacc cgactggtag aaggcgctac
     3001 gcccccagag acggcaggcc accgtctggt acggcagcca gtgattcacc atcaggtcga
     3061 gcgcgggtga gggagtttcg atcgagaggc ccccgagttc ctcgcgccac caccggtcgg
     3121 acgtcttccg ggccgcctcg atgtccgcgg cgtcctgata acgctcgagc agtccgcgag
     3181 cctcggcatg cgaggcggcg tctccaagga ggaacgccac ttccaccgtg ccgccggctg
     3241 gaagatcgat gaccgcccgc tggccgaaca tgggatcgag ccccgggccg gtccgtccgt
     3301 cgagaggctc gcccgagacg accgcgcgcg gcgcggccag ggtaccgtgc accccctgga
     3361 aggtggcgcg atccccgccg atcgacaatt cggccctggc gtcggtcgtg accacaccgg
     3421 ccaccgccac gcgcccggtc acgtcgcccc cacgccgggt cgtggcggtg aggcttccgg
     3481 ccgacggtcc cgccgccgtc gtcacgaacc ggccggaatc ctcgggcgcg tgcccgagga
     3541 cgaggcgcgc ggccgcgtac acggagagcc gccgggccgt gccgttccgc tccgtgatgc
     3601 ggatgcgcgt gacgcgtacc gggtcgtggc gcaccacgaa gatgtcggtc gacatgtcga
     3661 gtccgtcggc cgtgacatgc gcgcggctga cacccggcgc gtgccgcatt tcgtagtcgg
     3721 cgctccacgg cgccggcccc ggcaagggcg aaccgaatcg ccccgttgcc tcgtcgcgca
     3781 cgtagaagca ttcgcccggc acgtcgagga gcggatcgtt ggaccacggg gtcagccggt
     3841 actcacggct gttccggctc catgtcactc ccgcgcctgt ctcgctcacg atgcagccga
     3901 aattctcgtt ggcgaggacg ttggtccagg ggagaggcgt gcgccggaac gttccgtccg
     3961 accgccgctc catccggatg acgtattcgc ggccatcggc cgcgaaacct ccatggccgt
     4021 tgtcgaacgt cagagtgccg cgcgattgcc cgacggaggt ggcgacctcg tcgctcgcgg
     4081 aagtgacatc gcgtcttgcc gacggcgtgg ccgcggggct ccgatcggtt tccatgacgc
     4141 cgtatgccag tgtcggcagg ccttcccgga ggacgagcgc ggcccgatgg cgcaacgcca
     4201 tccgctcatc ttccgtcgcc gtgatgtcgc gcggcaccgc accgtcctcg gtgccggagg
     4261 tggccctcac gcgaatcgac tggatcggaa ggccaagcgc attccagtag cgggccatcc
     4321 ggtcgaagcc gcgtgcatgg ctcgcttcct ccgggagctc cgacgccggg tcgtgcaacg
     4381 cgagatgaca ccggccatcg agtgcgaaag gcgcgaggtt gatgtgcggg ccgggtaccg
     4441 gtgccgacag gtccccgagc gacgggacac cggccaggat cgcccccgcc accgcctcga
     4501 agtagtccgc atcggcgcga tcgcactcga ggcccatgcg acgcgcctct tccatctccc
     4561 gggcctcgtc gatccaggcg gcgacctggg ccggcaccgc cgcctgctcg gccagtcgac
     4621 gcgcttcctc gcgttcggcc gtcacgccga ccaggatgtc gatcgtccgc gcctcgccgg
     4681 gcgcgagagt cacccgtgcc gcctgggaaa ggaccgggtc gagcacgctc cctgcggttc
     4741 caggcgcgtt gtcctccgcc atcgcaaatc gcggcaactc accgggcagg cggcggccga
     4801 ccaggcgggc ccggtcggtt tcccagcgcc agccgctccc gccgtgcagc acctggaaca
     4861 tcgccgggta cgactcctcc ggtgaacggg ggcggcgggc ggccaggagg agccgcagtc
     4921 cgggctcgac gtccgtctgg aggaaaagac gcgagaaggt gggatgcgag gcgtgcgcct
     4981 gtgcttcgtg gaggaccacg tcggtcaccg tccgcacgac gaaattccgg ggatgatcgg
     5041 agcgattccg gagcacgagg cgacggagct cggcgtccgc gccgggaagg accgccacgg
     5101 ccaggcgcca ttcgatgtcg ttcttctcgc cggccaggtc gatccgaccg tccgatcggt
     5161 aactggcgct tgcgggcgcg tccgttcccc ccaatcgcca tgccgtgccg tcggccggtt
     5221 cctgaagcca cacatgactc ccttcggcgt ccgcggtgcg gtcggcactc catgcgctca
     5281 atcggagagt gccccactgt gaatagccgg tggcgaacgg cgtcatcagc aggtgatacc
     5341 ggccgttcga aaggaaacgg ccggcgaatg cgggtgccgt cttcggaccg aaggggggtg
     5401 cggcgggagg aatcatcgaa tccatcttga cagcttttcg gacgtgtgta aacgtttaca
     5461 tgaagacccg cttcggccaa acatccgtca tcttctttcc gggccgcaag tccaaccggg
     5521 ggtcgatcgt gaccacgatc cgtgatgtcg cccgcgcgtc cggcgtctcc atcgccaccg
     5581 tttcgcgcgt catcaatgac agcgcacgcg tcaacgacga cacgcgtcgc cgggtatggg
     5641 acgccgccat gcgcctcgat ttctggccga acggggccgc ccgcaccctc accacctcgc
     5701 gcacgcacgc catcggcgtc ctcctcccgg agttgcacgg cgagttcttt tcggaagtca
     5761 tccgggggat cgaccgggcc gcccgacgcg aacggctcca gctcctcatc tcgagctcgc
     5821 acgccgacac cgaggccatg ctggccgccg cccgcaccat gcgggggcgg gtggacggtc
     5881 tcatcgtgat gtcgcccgaa aagggatcgc aggcggccat cgagcagatc cgccgccgct
     5941 tcccgatggt gctcctcaac tcgagcgagc cggcccccga cgggcgttcc atcacggtgg
     6001 ccaacctcgt gggggcctac caggtggccc gccatgtcct cgacggcaac caccggcacg
     6061 tggcc
//
LOCUS       DASVOD010000479         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_656783, whole genome shotgun sequence.
ACCESSION   DASVOD010000479 DASVOD010000000
VERSION     DASVOD010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656783"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF720_10855"
     CDS             complement(<1..189)
                     /locus_tag="VF720_10855"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEX7879901.1"
                     /translation="MTDISGQGLISFERPIATPSEINRRVKLLIEGEFDDLWVRGEIS
                     NFTRAASGHLYFSLKDPGA"
     gene            complement(241..>2220)
                     /locus_tag="VF720_10860"
     CDS             complement(241..>2220)
                     /locus_tag="VF720_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879902.1"
                     /translation="IARILQGEPEWNALPARTPERIRGLLRRCLEKDAKRRLRDIGDA
                     RMEIEDVVAVHASSSAITAAAEAGTAAKDSRSLVEWGRLGAVALLALGAGWFLSRATQ
                     QQVPAQPIRFTVPEPEGVFLGADGAYIALSPDGRMLAFSVGDSGGTSQMWLRNLESTV
                     ATPVAGTEGVPPPYFWSPDSRRIAFGAQEKLKTIAVAGGDPETVCPIKSLRGGSWNRD
                     GVMLLAPYANGAIYRVAAGGGELTAVTAVDTTRGETAHRFPQFLPDGKHFLFSVLPGR
                     DGKIDTYVGSLESPKRQLLLSVASGVTWAPPGHLLYSREDRLIAQGFDLANLKLRGES
                     VSLGDVVNATEYSGGPIATASTTGVVAYGTFQLTGLRLAWTDFDGKELARIPLAPAPW
                     FGGSLSPDDRRAVLARRESANASSIWIADLERGVATRFTDEPGENEQPEWSPDGTRIA
                     HLWSENSAQRIKVRSLAGGVVVTLLEGDPLFKQFYGWTPDGQSLVYARLDPVTQWDLW
                     ILPMTGNQEPRPFVNTRFSELQARVAPDGRWLAYNSNESGRVEVYVQSFPVAGGKYQV
                     TNGGGTNFGWSHDGRQLHYGLASDPGIRFAADVLEGPEFRLGPPRRVITAPRDSPGFS
                     RDHTDTRLLAAFPAENDPGRSLTVILGGLPGASKR"
BASE COUNT          361 a          763 c          743 g          353 t
ORIGIN      
        1 ggcgccgggg tctttcaggc tgaagtagag atgccccgaa gcggcccgcg tgaaattgct
       61 gatctcgccg cgcacccaga ggtcgtcgaa ttcaccctcg atgagcagct tgacccggcg
      121 gttgatctcg gagggcgtgg cgatcgggcg ctcgaaggag atgaggccct gtccgctgat
      181 gtccgtcatg ctcgcctccc gtcacccgac aatgccgacc tcgaacggct ggcccggccg
      241 ctatcgcttc gacgcccccg gcagcccacc caggatcacg gtcaacgacc gccccgggtc
      301 gttctcggcc gggaaagcgg ccaggagccg ggtgtcagtg tgatcccggc tgaagcccgg
      361 gctgtcccgg ggcgccgtga tgacgcggcg cgggggcccg agccggaatt cggggccctc
      421 caggacatcg gcggcaaagc ggatgcccgg atccgaggca aggccgtagt ggagctgcct
      481 tccatcgtgg ctccagccga agttggtacc gccgccgttg gtcacctggt acttgccgcc
      541 cgcaaccggg aaggactgga catagacctc gacccgcccc gactcgttgg agttatacgc
      601 cagccaccga ccgtcgggcg ccacgcgggc ctggagttcg ctgaagcgag tattcacgaa
      661 gggccggggt tcctgattcc cggtcatcgg aaggatccag aggtcccact gcgtcaccgg
      721 atcgaggcgc gcatacacca ggctttgtcc gtcgggcgtc cagccgtaaa actgcttgaa
      781 aagtggatcg ccctccaaca gtgtaacgac cacccctccg gcgagggagc ggaccttgat
      841 ccgttgcgct gaattttcgc tccagagatg ggcgatgcgc gttccatccg gagaccattc
      901 gggttgttcg ttctcgcccg gctcgtcggt gaaccgcgtg gccacgccgc gctcgagatc
      961 ggcgatccag atactcgagg cgttcgccga ttcccgacgc gccaggacag cccgtcgatc
     1021 gtcgggcgaa agcgatccgc cgaaccaggg agcgggcgcc agcggaatgc gcgccagctc
     1081 cttgccgtca aagtccgtcc aggccagtcg caggccggtc agctggaagg tgccgtaggc
     1141 cacgacacct gtcgtggacg ccgtggcgat cggaccgccc gagtactccg tcgcgttgac
     1201 gacatcgccc agcgacacgg actcgccacg gagtttcaag ttggcgaggt cgaagccctg
     1261 ggcgatcaac ctgtcctcgc gggagtacag gagatgtccg gggggcgccc aggtgacgcc
     1321 gctggccacg gacaggagca gctgccgttt tggcgattcg agcgaaccga catacgtatc
     1381 gatcttgccg tcccggcccg ggagtacgga aaacaggaag tgcttgccgt ccggcaggaa
     1441 ttgcggaaat cgatgggcgg tctcgccccg ggtcgtgtcc acggcggtca ccgccgtgag
     1501 ctcgccgccg cccgccgcca cgcggtagat ggcaccgttc gcataaggcg ccaggagcat
     1561 cacgccgtcc ctgttccagc tgccgccgcg aagcgacttg atcgggcaga cggtttccgg
     1621 gtctccgccg gccacggcga tcgtcttcag tttctcctgc gcgccgaagg caatccggcg
     1681 gctgtctggc gaccagaagt acgggggcgg aacaccttcc gtcccggcca cgggcgtggc
     1741 cacggtcgat tccaggtttc gaagccacat ctgcgatgtc ccgcccgagt ctccgacact
     1801 gaaggccagc atgcggccgt cgggcgacag tgcgatgtac gctccgtccg cgccgagaaa
     1861 caccccctcg ggctccggca ccgtgaaacg aatcggttgc gccggcacct gctgctgcgt
     1921 cgcgcgcgac aggaaccaac cggcgccgag cgccagcagc gccacggctc cgaggcgccc
     1981 ccactcgacc aggctgcgtg agtctttcgc cgccgttccc gcctcggcgg ccgcggtgat
     2041 ggccgaactg gaggcgtgca ccgccaccac atcctcgatc tccatccggg catcgccgat
     2101 gtcgcggagg cggcgcttcg cgtccttttc gaggcagcgg cgcaggagcc cgcggatccg
     2161 ctcgggcgtg cgcgcgggga gcgcgttcca ctccggctct ccctggagga tgcgcgcgat
//
LOCUS       DASVOD010000480         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1025522, whole genome shotgun sequence.
ACCESSION   DASVOD010000480 DASVOD010000000
VERSION     DASVOD010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025522"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF720_10865"
     CDS             <1..526
                     /locus_tag="VF720_10865"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX7879903.1"
                     /translation="FNKSKDLWGSYAYDEKNDALRVTVKPVAHEMTEWMMFSIPEVTT
                     DKATVALDWEKLRVPFTIEVKTVDTALASARAAMANLKADDNTTAYRAAAFAFDNNVA
                     LDEAAMWVDKSIAIKPTWLNHRLKANMLAAKGDKKGAIEHATASIEVGKKDEAPQEEL
                     DKMQKLIDDWKKSM"
     gene            complement(527..2236)
                     /locus_tag="VF720_10870"
     CDS             complement(527..2236)
                     /locus_tag="VF720_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012926238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX7879904.1"
                     /translation="MSLVDWIVLVGALAGMVSYGMWRGRKAASLDGYLLAGRDMRWGT
                     VCLSIMATQASAITFLSTPGQAYADGMRFVQFYFGLPLAMVVLSVTAVPIFHRLKVYT
                     AYEYLERRFDRKTRTLAAGLFLIQRGLACGLTIYAPALILSVLLGWSLPWTIVAIGTL
                     VVSYTATGGTRAVNHTNSLQMVIVLSGILAVLVATVSRMPSGVSFGDAVWLAGTMGKL
                     NAIDTSFDWNNRYNLWSGLIGGFFLALSYFGTDQSQVARYLSGQSVTQSRLGLLANGL
                     VKVPMQFVILFTGAMVFVFYLFATPPVFFNSVAVNTVKNSPQAAEFAAAEKDFQAAHD
                     DRREALESLADLRIAPATQPMLADTKADLMGRVHAADEAATEARTRAVTAIQAADPKV
                     APRDVNYVFLTFVLKNLPIGMIGLVLACVFAAAMSSTAGELNSLASTSVVDFYKRSFR
                     PEAGDRELLTVSRVSTVIWGAFAVLFAEFAGKMGSLVEAVNILGSLFYGTILGIFLLA
                     FYVKRVGGTAVFMGALIGEAVVVGFFSFSTISFLWYNVIGCLATMGAAWLLSLRRAAP
                     RNA"
     gene            complement(2233..2385)
                     /locus_tag="VF720_10875"
     CDS             complement(2233..2385)
                     /locus_tag="VF720_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879905.1"
                     /translation="MARSPIDASNRPHGDEGPPGGGSWNRWYALVIGWLVVQILLYWL
                     FTKAFS"
     gene            complement(2415..>2712)
                     /locus_tag="VF720_10880"
     CDS             complement(2415..>2712)
                     /locus_tag="VF720_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879906.1"
                     /translation="VTFLDPASPLLTAPNRIGETDFAGWVQERGLYFASEWDARYTPL
                     LASNDPGEPERKGGLLVAHTGKGVFIYTGYAFFRQLPAGVPGAIRLFVNLVSAE"
BASE COUNT          537 a          876 c          923 g          376 t
ORIGIN      
        1 cttcaacaag tcgaaggacc tctgggggag ctacgcctac gacgagaaga acgacgcgct
       61 ccgggtcacc gtgaagcccg tggcccacga gatgaccgag tggatgatgt tttccatccc
      121 cgaggtgacg acggacaagg ccaccgtggc gctcgactgg gagaagctcc gcgtgccgtt
      181 cacgatcgag gtcaagacgg tcgacaccgc cctggccagc gcgcgggcgg ccatggccaa
      241 tctcaaggcc gacgacaaca ccaccgccta tcgcgcggcg gccttcgcct tcgacaacaa
      301 cgtggcgctg gacgaggccg cgatgtgggt cgacaagtcg atcgccatca agccgacctg
      361 gctcaatcac cgcctgaagg ccaacatgct ggccgccaaa ggcgacaaga agggcgcgat
      421 cgagcacgcg acggcgtcca tcgaggtcgg caagaaggac gaggccccgc aggaagagct
      481 cgacaagatg cagaagttga tcgacgactg gaagaagtcg atgtaatcag gcgttccggg
      541 gggcggcgcg gcgaagcgag agcagccacg ccgcccccat cgttgccagg caaccgatca
      601 cgttgtacca gaggaacgag atcgtcgaga acgagaagaa gcccaccacc accgcctccc
      661 cgatcaacgc ccccatgaag accgccgtcc cgcccacgcg cttcacgtag aacgccagca
      721 ggaagatgcc gaggatcgtg ccgtagaaga ggctgccgag gatgttgacg gcctctacca
      781 ggctccccat cttccccgcg aattcggcga agaggacggc gaaggctccc cagatgactg
      841 tcgaaacgcg cgaaaccgtg agcagctccc ggtcgcccgc ctccggccgg aaactccgct
      901 tgtagaaatc gaccacgctc gtactggcca gtgaattgag ttcgcccgcc gtggaggaca
      961 tggcggccgc gaagacgcag gcgaggacga ggccgatcat gccgatcggc aggttcttca
     1021 ggacgaacgt caggaaaaca tagttgacgt cgcgcggcgc caccttcggg tcggccgcct
     1081 ggatggcggt gacggcgcgc gtgcgtgcct cggtggcagc ttcgtcggcg gcatggacac
     1141 gccccatgag gtcggccttc gtgtcggcca gcatcggctg cgtggccggc gcgatccgga
     1201 ggtcggccag cgactcgagc gcctcgcgcc gatcgtcgtg cgccgcctgg aaatcctttt
     1261 ccgcggcggc gaactcagcc gcctgcggtg aattcttcac cgtgttcacc gccaccgagt
     1321 tgaagaagac cggcggcgtg gcgaagaggt agaaaacgaa gaccatcgcc ccggtgaaga
     1381 ggatgacgaa ctgcatcggc accttgacga ggccgttggc caggaggccc agccggctct
     1441 gcgtgaccga ctgtcccgag aggtagcgcg ccacctggct ctggtcggtg ccgaagtacg
     1501 aaagcgccag gaagaagccc ccgataaggc ccgaccagag gttgtagcgg ttgttccagt
     1561 cgaacgaggt gtcgatggcg ttcaacttcc ccatcgtgcc ggccagccag acggcgtcgc
     1621 cgaatgacac gcccgacggc attcggctga cggtggccac gagcaccgcc aggatcccgg
     1681 agaggacgat gaccatctgc agcgagttgg tgtggttcac cgcgcgcgtg ccgccggtgg
     1741 cggtgtagct tacgaccagc gtgccgatgg ccacgatcgt ccaggggagg ctccaaccga
     1801 ggagcaccga gaggatgagc gccggcgcgt agatcgtgag gccgcaggcc aggccgcgct
     1861 ggatgaggaa gaggccggcg gccagcgtgc gcgtcttccg gtcgaagcgc cgctccaggt
     1921 actcgtaggc ggtgtacacc ttgagtcgat ggaagatcgg caccgcggtg accgacagca
     1981 ccaccatcgc cagcggcagg ccgaagtaga actgcacgaa tcgcatgcca tcggcgtagg
     2041 cctgcccggg cgtgctcagg aaggtgatgg cgctggcctg tgtggccatg atcgagaggc
     2101 acaccgtgcc ccagcgcatg tcgcgccccg ccaggaggta gccgtcgagg ctggcggcct
     2161 tgcggccgcg ccacatcccg tacgacacca tgccggcgag cgcgccgacc aggacgatcc
     2221 agtcgacgag gctcacgaga aggccttcgt gaagagccag tagaggagga tctgaacgac
     2281 aagccagccg atcacgagcg cgtaccagcg gttccagctc ccgccgccgg gcgggccctc
     2341 atcgccatgc gggcggttgg acgcgtcgat cggtgaccgt gccacgggtc ggttgtccga
     2401 cacggggcgg cggctcattc ggccgagacg agattcacga agaggcggat ggcgcccggc
     2461 acaccggcgg gaagctgccg gaagaaggcg tagccggtgt agatgaagac acccttcccc
     2521 gtgtgcgcca cgaggagacc ccccttccgc tccggctcgc ccggatcgtt gctggccagg
     2581 agcggcgtgt acctggcatc ccactccgag gcgaagtaga ggccgcgctc ctgcacccat
     2641 cccgcaaagt ccgtttcgcc gatccggttg ggcgcggtga gcaacgggct cgccgggtcg
     2701 agaaacgtga cg
//
LOCUS       DASVOD010000481         8122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_971580, whole genome shotgun sequence.
ACCESSION   DASVOD010000481 DASVOD010000000
VERSION     DASVOD010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971580"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VF720_10885"
     CDS             <1..1030
                     /locus_tag="VF720_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX7879907.1"
                     /translation="TRPTEATRRAIAGAEVGDDVYGEDPTVLELERETALLLGKPAAL
                     FVPSGTMGNEIAIVVHGRPGREVVLDSQSHIMTAEGGGPAVLAGVTLRPLASDTGRLD
                     PDRVASALVLDDDPHHAPTVLACAENTHNRLGGRVFPPDVFTELAALLGRHQIPLHLD
                     GARLWNAAVASGLSAAELAGPADSVMVCFSKGLGAPVGSALAGSEAFIHEARRVRKLL
                     GGGMRQAGILAAGCRFALEHHRERLSEDHRRARRLAELVPLPVGAAILGGRPETNIVI
                     WDLPPDRFDVFEIRRRLAADDIHLSDFGPGRLRAVTHLDVDDAGIERVAASLADLLAE
                     AGTRVVYR"
     gene            1027..1620
                     /locus_tag="VF720_10890"
     CDS             1027..1620
                     /locus_tag="VF720_10890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7879908.1"
                     /translation="MKTALKERARRLAEPLVKFLHKLGVTPDQITFVGLGLSLAAAVA
                     AGLGRFGPATVLLLLGSVCDMLDGGLARLSGQGTRFGAFLDSTIDRYAELGLFIGYAV
                     YFADQGTPLDVAVVCTAAAGSILVSYARARAEGLGIDCNVGLMERPERLVLLIAATAL
                     GPVALLWALRLLAVLTHVTALQRIRHVHGRLRSEPRG"
     gene            1617..2264
                     /gene="tmk"
                     /locus_tag="VF720_10895"
     CDS             1617..2264
                     /gene="tmk"
                     /locus_tag="VF720_10895"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX7879909.1"
                     /translation="MNPHGLFVTFEGIEGSGKSTQLRRAATWLAERGHRVVTTREPGG
                     TRMGEAIRAMLLDPSSELGGEAELFLYLADRAETMRQVIEPGLALGAVVLGDRHTDAT
                     LAYQGAMRGIGLERARDLNALATRGRRPHLTLLFDLDAAIGLGRAGGRRGSGSPDRLE
                     AEAPPFHDGVRRAYLALAAAEPERMRVVNAAAAPDVVWEEVRRHLESIVPPRFPS"
     gene            2261..3025
                     /gene="larB"
                     /locus_tag="VF720_10900"
     CDS             2261..3025
                     /gene="larB"
                     /locus_tag="VF720_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEX7879910.1"
                     /translation="MNEARLRKLLEAVRGGQLSLDGAIESLRHLPYEELPDLAIDHHR
                     ELRCGFPEVVFALGKSDAQVEAATRAVAARSASVLVTRVTPTLAGRLVAEIPSLRHDA
                     EARAVYRHAESPAPGGRVAVVAAGTADVPVAAEAALTASLFGNEVSRVTDVGVAGLHR
                     LLPRIAGLRRHHAVVVVAGMEGALPSVVAGLIDVPVIAVPTSVGYGASFGGVTALLGM
                     LNSCAAGVTVVNIDNGYGAGHAAGLIARGRATTGSS"
     gene            3025..4353
                     /gene="larC"
                     /locus_tag="VF720_10905"
     CDS             3025..4353
                     /gene="larC"
                     /locus_tag="VF720_10905"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEX7879911.1"
                     /translation="MRHLHFEQVGGVSGDMLLGALVGLGVDPGSLESALRSLGLPGLS
                     LRVRAVERNGAPATRVEVLIDGRPADPHDHGHAHGHAHSHDAHSHGHAHSHDTHSHGQ
                     AHSHEHRRLRDIEAMIDASSLPDAVRRDSLAIFRRLAEAEAAVHGTSVEEVHFHEVGA
                     VDSIADVVGAVWSIHHLEVDRVTGGPFVLGRGEVAMAHGRWPVPAPATVRLIEGLPVR
                     FVDLEGETVTPTGAAVLSSLATFVLDLPVMTLERSSAGAGGREWPDRPNILRAFLGVT
                     EKQAPVAGTRMETIAVLGTQIDDMTPQGIADLADRLMEAGALDVTVTPLLMKKGRPGF
                     GLSVYSSVREAGRMATLLLTVSSTLGVRVRMEERHVLARRVITVATRWGPVRVKVTHR
                     PDGTIEGHPEFDDCQEISHRAHKSLHYVVEEARRAAAEALGGPENPLSGP"
     gene            4846..8091
                     /locus_tag="VF720_10910"
     CDS             4846..8091
                     /locus_tag="VF720_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879912.1"
                     /translation="MRKVLGISLALVVALTTIASAKPVDRISDLRPEVPDQTLESNSG
                     NPGLSSHIFTDTVGYGGTYWAADSLRWEAIRDSHWSFDTGVGSSINGGANVNKPVGYH
                     QTMEGWIGIDQTLSQLPYFRRSSTCAIAGSFSFWAGVTLTEANALCFAAGQGYGNSWT
                     LTLSKTFAYSGSGTVSFSYQYAVEAEPGFDYAYALIDTTGNGSQDDIILATYTGTAAG
                     TEGPITLNAGQDMRSDAGNYTIKFVAASDGGYSDEDGAYPTTCGLMVVDNVTVGADVS
                     NFEAGANGWVQEVPTTAVGDFSNISDLGDLVPPAVFCPCGLADSVLVFFDTNDGHPLD
                     QDNIAMSPWIDLLAGGDAGRPGKLMLYDVYALMPLANYIFVQLRVRYYPEICSATGLI
                     IRTPWKDQNTIFYFGEAPFCNPVTAPRARDYSAVFSPGAQQAQVAWGMLSLCRTSPFG
                     PQCTGQTNQTPYIDNISLGVYGNAAVPVVNTTTFDRYQDNFAADGTLNVASPGRFDQN
                     RIKGASTPSTGTILGDTLNARGNGGNTEVRLVFRVRTGPFVNGATLASYTARWTAEPT
                     LNARYGGTWYSARMDTAEQAGIPATSPFTWMSTFHEADPGFLGTDTAADPNDVGRLAN
                     DIIPDHLFTPGSRLDYFVASRYRPPDPRNPGGTTWATDPDTIGATFREAEILPSSLAA
                     DSTWNCVLYVDHHDDRDGADQAAEETGLRNSLGLGGNNFEGTRHDRFDNQTPSSAQLS
                     FGRPLSTNYGASQVQIFAYKAVAWHGGTLTSGVLTTQDAAILQPWLVIRAIGNNNFWG
                     SGEGIMQSMQGEGGIARTFMNNTLGVIQDCTSIRLAGCGGAGIDTTFCIPTSQVAGAH
                     FTSTILPRGRGNGCPNLLSFDRLDNNGAVATARGQLNYRRLVPGQGGVLTDIEFESVT
                     NHNTVDVTYKTVLDGISVGRMRTNAGFFGVGCDDLGASYARTDNILDWFGAASICRLP
                     AALVDVPVNGGPQPPAFRHALGNAYPNPMNPTTRIQFTNGVANGRVKVEIFDITGRLV
                     RSLVDAKMPAGVHEVTWDGANEGGSSVPSGMYFYRMTADDFVSAKKLVVSK"
BASE COUNT         1282 a         2805 c         2622 g         1413 t
ORIGIN      
        1 gacccgcccc accgaggcga cgcgccgtgc gatcgccggc gccgaggtgg gggacgatgt
       61 ctacggagag gacccgaccg ttctcgaact ggagcgcgaa accgccctcc tgctcgggaa
      121 gccggcggca ctcttcgtgc cgtcgggcac gatggggaac gaaatcgcca tcgtcgttca
      181 cgggcggccc gggcgggagg tggtcctcga ctcgcagagc cacatcatga ccgccgaggg
      241 cgggggcccg gcggtgctgg ccggcgtcac gctccggccg cttgcatcgg acacaggtcg
      301 cctggatccc gatcgcgtcg cgtcggctct ggtgctcgac gacgatccgc atcacgcgcc
      361 gaccgtgctg gcctgcgccg agaacacgca caatcggctg ggcggacgcg tttttccgcc
      421 cgacgtcttc actgaattgg cggcactact cggccgtcac cagattcccc tgcacctcga
      481 cggggcgcgg ctgtggaacg cggcggtcgc atcgggtctt tccgccgccg agctggccgg
      541 gccggccgac agcgtgatgg tctgcttctc gaaggggctc ggcgcgccgg tcggatcggc
      601 gctcgccggc tcggaggcgt tcattcacga agcgcgacgc gtccgcaagc tcctgggcgg
      661 cgggatgcgc caggcgggga tcctggcggc cggctgccgc ttcgcgctgg agcatcatcg
      721 tgagcgtctg tccgaagacc accggcgcgc gcggcgcctg gccgagctcg tgccgctgcc
      781 ggtgggagcc gccatactgg gcggacggcc cgagaccaac atcgtcatct gggacctgcc
      841 gcccgaccgg ttcgacgtgt tcgaaatccg tcggcgcctg gccgcggatg acatccacct
      901 gtccgacttc ggaccgggaa ggctccgggc cgtcacgcac ctggacgtgg acgacgcggg
      961 gatcgagcgg gttgccgcct cgctggccga cctgctggcc gaagcaggga ctcgagtggt
     1021 gtaccgatga agacggccct caaggagagg gctcgccggc ttgcggagcc gctggtgaag
     1081 ttcctccaca agctcggtgt cacccccgac cagatcacct tcgtcggcct cggcctctcc
     1141 ctcgccgccg cggtggcggc ggggcttggc cggttcggac cggccaccgt cctcctcctg
     1201 ctcggcagcg tgtgcgacat gctcgacggt ggcctggccc gcctttcagg gcaggggacg
     1261 cgcttcggcg cgttcctcga ctccaccatc gacaggtacg ccgagctggg actcttcatc
     1321 ggctacgccg tctattttgc cgaccagggg acgccgctcg acgttgccgt ggtgtgcacg
     1381 gcggcggcgg gatcgatcct cgtttcgtac gcgcgtgccc gggccgaggg gctcggtatc
     1441 gactgcaacg tgggactcat ggaacgaccg gaacgactgg tcctcctgat cgcggccacc
     1501 gcgctcggac ccgtcgccct gctctgggcg ctgcggctgc tcgccgtact cacccacgtg
     1561 accgcgctgc agcgcatccg gcacgtgcac ggacggctcc gctcggaacc gcgcggatga
     1621 acccgcacgg cctgttcgtc acctttgaag ggatcgaggg ctccggcaag tcgacccagc
     1681 ttcgaagggc cgccacctgg ctggcggaac ggggtcaccg tgtcgtaacg acgcgggagc
     1741 ccgggggaac ccggatgggg gaagccatcc gggccatgct cctcgacccg tcctcggagc
     1801 tgggcggaga agccgagctc ttcctctatc tcgccgatcg cgccgagacg atgcgccagg
     1861 tcatcgagcc gggcctggcg ctgggggccg tcgtgctcgg agaccgccac acggacgcca
     1921 ccctggctta ccagggcgcc atgcggggga tcggcctgga gcgcgcccgc gacctgaacg
     1981 cgctcgccac gcggggacga cggccgcacc tgaccctcct cttcgatctc gacgcggcga
     2041 tcggactcgg gcgcgccggc ggtcgccgcg gctccggctc acccgatcgg ctcgaagcgg
     2101 aagcaccgcc gttccacgat ggcgtgcgcc gggcctacct cgcgctggcg gccgccgaac
     2161 cggaacggat gcgcgtggtg aacgccgccg cggcaccgga cgtggtgtgg gaggaggtgc
     2221 ggcgacacct cgagtcgatc gtgccgcccc ggttcccgtc gtgaacgaag cccgacttcg
     2281 caagcttctc gaagccgtgc gcggcggcca gctgtcgctc gacggcgcca tcgaaagcct
     2341 gcgccatctt ccctacgagg agcttccaga cctggccatc gatcatcacc gcgagttgcg
     2401 ctgcggcttt cccgaggtgg tcttcgcgct cggcaagtcg gacgcgcagg tcgaagcggc
     2461 cacccgggcc gtggccgccc gatcggcttc cgtcctggtg acgcgggtga cgcccaccct
     2521 cgccggccgg ctggtcgccg aaattccgtc ccttcggcac gatgccgagg cgcgcgccgt
     2581 gtaccgccac gccgagtcgc cggctcccgg agggcgggtt gccgtagtgg ccgccgggac
     2641 cgccgatgtt ccggtcgcgg ccgaggccgc tctgaccgca tctctcttcg gcaacgaggt
     2701 gtcgcgcgtc accgatgtcg gtgtggcggg ccttcaccgg ctcctgcccc ggatcgccgg
     2761 gctgcggcgg caccatgccg tggtcgtcgt ggccgggatg gaaggggctc tgccgagtgt
     2821 cgttgccgga ctgatcgacg tgccggtcat cgccgttccg acgagtgtcg ggtacggggc
     2881 ctcgttcggc ggcgtgaccg cgctgctggg gatgctcaat tcgtgtgcgg ccggcgtcac
     2941 cgtggtgaac atcgacaacg gatacggcgc gggacacgcc gccgggctca tcgcaagggg
     3001 acgcgccacg acggggagtt cctgatgcgc catctccatt ttgaacaggt cggcggcgtc
     3061 agcggtgaca tgctgctcgg tgccctcgtc ggcctcggcg tggatcccgg ttcgctcgag
     3121 tccgcgctcc gttccctcgg ccttcccggc ctctcgcttc gcgtccgcgc cgtggagcga
     3181 aacggcgcgc cggccacgcg cgtggaagtg ctgatcgatg gtcgccctgc cgatccccac
     3241 gatcacgggc acgcgcatgg ccacgcacat tcgcacgacg cacactcgca cggccacgca
     3301 cattcgcacg acacacactc gcatggccag gctcactccc acgaacaccg ccggcttcgt
     3361 gacatcgagg cgatgatcga cgcttcatcg ctcccggacg ccgttcgccg cgactcgctg
     3421 gcgatcttcc gccggctggc cgaagccgag gcggcggttc acggcacgtc ggtggaggag
     3481 gtccacttcc acgaggtcgg cgccgtcgat tcgattgcgg acgtggtggg ggcggtctgg
     3541 tcgatccatc acctcgaggt cgatcgggtg accggcggac ccttcgtgct ggggcgcggg
     3601 gaggtggcca tggcccacgg acgttggccc gtcccggccc cggccaccgt ccggttgatc
     3661 gaaggtctgc cggtccggtt cgttgacctg gagggtgaaa ccgtgacgcc gaccggggcg
     3721 gcggtcctct cctcgctggc caccttcgtg ctcgacctcc ccgtcatgac tctggaacgg
     3781 tcgtcggccg gcgccggcgg ccgcgaatgg ccggatcgcc ccaacatcct gcgagccttt
     3841 ctcggcgtga ccgaaaagca ggctccggtg gccggtacca ggatggagac gatcgccgtg
     3901 cttgggacgc agatcgacga catgacaccc caggggatcg ccgacctcgc cgaccgcctg
     3961 atggaggcgg gtgccctgga cgtgacggtg acgccgctcc tcatgaagaa ggggaggccg
     4021 gggttcggac tttcggtcta ctccagcgtc cgggaggcgg ggcggatggc caccctcctc
     4081 ctgacggtca gctccaccct gggtgtccgg gtccgcatgg aggagcgtca tgtcctggcc
     4141 cgccgggtga tcacggtcgc cacccggtgg ggcccggtcc gggtcaaggt gacccaccgc
     4201 ccggatggga cgatcgaggg tcacccggaa ttcgacgact gtcaggaaat cagtcaccgc
     4261 gcgcacaagt cgttacatta tgtagtggaa gaggctcgcc gggcggccgc tgaggcgctc
     4321 ggagggcccg aaaatcctct ttccgggcca tgaaactgta ccgatttgag tacagacacc
     4381 ccggcgcgca agctggataa tgccttatat tatcggccca tcgtgagtta ccaagtttct
     4441 caggattgac atggtcaggt ggatactcta gactcagtac aggtttggta cgtcgcagtc
     4501 cgtccattag tcaggcaggt gccagcgcaa gcgccccatg aaaaggggaa ggcgaatggt
     4561 tctgcactca ggaccgaaac tcacacacac acggcctgag acggcggaga cagtaccacc
     4621 aggttcaaca ggctaaggcg ctacaccctg acacccatcg ctccaggacg tggtggcccg
     4681 atcgccgatc cgtgcaacag gttccctgcc atcttctggc acaagtcgaa aggggggtcc
     4741 taccaaccgc aagaacggat ggatgtcacc gtccatcacg aggctgcgat tctcactcga
     4801 cgtgcaacag tagatagcct gcgatgcaac gatggaggta ttagcatgcg taaggttctg
     4861 ggtatttccc tcgcgctcgt cgtagccctg acgacgattg cgtcggctaa gcccgtggac
     4921 aggatttccg atcttcgtcc cgaggttccc gatcagaccc tcgaatcgaa ttccggcaat
     4981 cccggcctct cgtcccacat cttcacggac acggttggat acggtggtac ctactgggcc
     5041 gccgactctc tccgctggga ggccatccgc gacagtcact ggtcgtttga cacaggcgtc
     5101 ggctcgtcaa tcaacggcgg tgccaacgtc aacaagccgg tcggctacca tcagacgatg
     5161 gaaggctgga tcggcatcga ccagactctc agccagcttc cctatttccg ccgctcgagc
     5221 acgtgtgcca tcgccgggtc cttctccttc tgggccggcg tcaccctcac cgaggcgaat
     5281 gctctctgct tcgccgccgg ccaggggtat ggcaacagct ggacgctcac gctttccaag
     5341 acctttgcct attcgggtag tggaaccgtc agcttcagct atcagtacgc ggtggaagcc
     5401 gagcccggtt tcgattacgc ctacgcgctg atcgatacca ccggaaacgg ttcgcaggat
     5461 gacatcatcc tggccaccta caccggtacg gctgccggca ccgaaggccc gatcacgctg
     5521 aacgcgggcc aggacatgcg ctccgacgcc ggtaactaca ccatcaagtt cgtggccgcc
     5581 tccgacggtg gctattcgga cgaagacggt gcgtatccga cgacctgcgg cctcatggtt
     5641 gtggacaacg tcaccgttgg tgccgacgtc agcaacttcg aagccggtgc gaacggctgg
     5701 gtgcaggaag ttccgacgac tgccgtcggc gacttctcga acatctccga cctcggagat
     5761 ctcgtgcctc ccgccgtgtt ctgcccctgc ggtcttgccg actccgttct ggtcttcttc
     5821 gacacgaacg acgggcaccc gctggatcag gacaacatcg cgatgtcgcc ctggatcgac
     5881 ctgctcgccg gtggcgacgc gggccgtccc gggaagctca tgctgtacga cgtctacgcc
     5941 ctcatgccgc tcgcgaacta catcttcgtg cagctgcgtg tccgctacta tccggaaatc
     6001 tgctccgcca ccggtctgat catccggacc ccgtggaagg atcagaacac gatcttctac
     6061 ttcggtgaag cgccgttctg taaccccgtc accgcgccgc gcgcccgcga ctactcggcc
     6121 gtcttctcgc cgggtgccca gcaggctcag gttgcgtggg gtatgttgag cctctgccgt
     6181 acctcgccgt tcggtccgca gtgtacgggt cagaccaacc agaccccgta catcgacaac
     6241 atctcgctcg gcgtctacgg caacgcggcc gttccggtcg tcaacaccac gaccttcgac
     6301 cgctaccagg acaacttcgc cgccgacgga acgctcaacg tggcgtcccc cggccgtttc
     6361 gaccagaacc gcatcaaggg tgcctcgacg ccgtcgaccg ggacgatcct gggtgacacg
     6421 ctgaacgccc gcggcaatgg tggaaacacc gaagttcgtc ttgtcttccg cgtccgcacc
     6481 ggtccgttcg tgaacggcgc caccctcgcg tcctacacgg cgcgctggac ggcggagccg
     6541 accctgaatg cccgctatgg cggcacctgg tactcggctc gcatggacac cgcggagcag
     6601 gccggcatcc cggcgacttc tccgttcacc tggatgtcga cgttccacga ggccgacccc
     6661 ggcttcctcg gcacggacac cgctgccgac ccgaacgacg tgggtcgtct cgcgaacgac
     6721 atcattcctg accacctgtt cacccccggt tcgcgtctgg attacttcgt ggcgtcccgc
     6781 tatcggccgc cagatcctcg caacccgggt ggcacgacgt gggcgaccga tcccgacacc
     6841 atcggggcga cgttccgcga agccgaaatc cttccgtcct ctctcgcggc tgactcgacc
     6901 tggaactgcg tgctctatgt cgatcaccac gacgaccgcg acggtgcgga ccaggctgcc
     6961 gaagaaacgg gtctccgtaa ctcgctcggc cttggcggca acaatttcga aggtacccgc
     7021 cacgaccgtt tcgacaacca gaccccgtcc tcggcccagc tctccttcgg ccgtccgctc
     7081 tccacgaact acggcgcgag ccaggtccag atcttcgcct acaaggcggt ggcatggcac
     7141 ggcggaacgc ttacttcggg cgtgctgacg acgcaggatg cggccattct ccagccgtgg
     7201 ctcgtgatcc gtgccatcgg caacaacaac ttctggggct ccggcgaggg catcatgcag
     7261 tccatgcagg gtgagggcgg catcgctcgc accttcatga acaacacgct cggcgtcatc
     7321 caggattgca cgagcatccg tctcgcgggt tgcggtggtg ccggtatcga caccacgttc
     7381 tgcatcccga cgagccaggt tgccggcgcg cacttcacca gcacgatcct tccgcgtggt
     7441 cggggcaacg gctgcccgaa cctcctgtcg ttcgatcgtc tggacaacaa cggcgccgtc
     7501 gccactgccc gtggccagct gaactatcgc cggctggtcc ctggtcaggg tggcgtgctg
     7561 accgacatcg agttcgagtc ggtcaccaac cacaacacgg ttgacgtcac ctacaagacg
     7621 gttctcgacg gtatttcggt cggtcgtatg cgtaccaacg ccggcttctt cggcgtcggt
     7681 tgcgacgacc tgggcgcctc gtacgcccgt accgacaata tccttgactg gttcggcgcg
     7741 gcctcgatct gccggctccc ggcagccctg gtcgacgttc ccgtcaacgg tggcccgcag
     7801 ccgccggcgt tccgtcacgc tctcggcaat gcctacccga acccgatgaa tcccaccacg
     7861 cgtattcagt tcaccaacgg cgtggccaac ggtcgtgtga aggtcgagat cttcgacatc
     7921 accggtcgcc tggtccgttc gctggtggat gcgaagatgc cggctggcgt tcacgaggtc
     7981 acgtgggatg gcgccaacga gggtggctcg tccgttccga gcggcatgta cttctaccgc
     8041 atgacggctg acgacttcgt ttcggcgaag aagctggttg tctccaagta agaagtacgg
     8101 tcgccggagc aacatccggt aa
//
LOCUS       DASVOD010000482         6740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_136941, whole genome shotgun sequence.
ACCESSION   DASVOD010000482 DASVOD010000000
VERSION     DASVOD010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136941"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VF720_10915"
     CDS             <1..575
                     /locus_tag="VF720_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879913.1"
                     /translation="SDVRRESDLRRESDVRREPEVRREPETRREMEARHEAELRREVE
                     PVRERIEFTRPVSRLAATLASESWAEAPLDAVPYVEPDVDIETRRLKDAQAVAMQRVR
                     ENLREVGAAAQEKSGPVELDPEAVAAGHLDQEPERIPFVEARLSQAEDLVRTAARAAV
                     ANRTQDDRWKSSYTRDNLDVPAFMRRPPTE"
     gene            706..1479
                     /locus_tag="VF720_10920"
     CDS             706..1479
                     /locus_tag="VF720_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX7879914.1"
                     /translation="MTRTRIQSGPVPASGAGPARLADPPPLLTDNPALIGSRRSRPLV
                     REDILDAVDDQLERVGYARMTIEEVARLAGVGKGSIYLHFASKEDLALSSIDRLVRRL
                     LDRLAEIAGEPLPAADRLRRMLETRVLFRFDHVNQDAVSLDEMLAILRSALLQRREEW
                     FERESQLLARVISEARAAGDAKDAPTYDHALRDARALVTATNALLPYSLSKSELGRRS
                     LLVDRVIAVADLLLCGLSLDSTASGPNHDPIRQDRGTNP"
     gene            1599..2666
                     /locus_tag="VF720_10925"
     CDS             1599..2666
                     /locus_tag="VF720_10925"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7879915.1"
                     /translation="MPAPVDRPTTAAANGAILDSLTTHLNAWYVYPETAAKMVKSIES
                     HRGKLEKITSSIELADALTAEMQDISHDKHLRVVYREREIPVRAVSEEFTPAERERQR
                     KESRLRNFGFEKVERLMGNVGYLEMRSFAQVADGAAEAATAAMNFLGNVDALIIDLRR
                     NGGGSPEMVALVTSYLYPAGERIHLNDLYWRPGDTTEQFWTQPHVPGPRLDGKDVYIL
                     TSNRTFSAAEEFCYNLKNLARATIVGENTGGGAHPGTMVRLSDHFEMFLATGRAINPV
                     SKTNWEGTGVPADMTCPADQALSVAHVTAIEKLLAKASDPDDQQRLAMALDAVKAQSA
                     TPAGGEVAPPAGPARIVVRQN"
     gene            2692..4347
                     /locus_tag="VF720_10930"
     CDS             2692..4347
                     /locus_tag="VF720_10930"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7879916.1"
                     /translation="MTLVPVKMRRAHRCKPRRLPVLLVFVVALSLFGRFPAPAQAASP
                     TIPDTPIGAVLSEMLDVIRSGDIEKARDFVTRRYAAEALAARPMESRLDGWRDLMGSL
                     AQPKLLEVRTGSGNPAMIIESGATGEVFEVVVRPAPGVPDKITGVRMQSTIPGKPAIP
                     AGPITDKERSALIDQLLDRLVADDRFSGVVMLAKDGKPAYQRATGMANQSWSIPNRID
                     TKFCLGSMNKMFTSLAVARLVQDGKLAYGDKVGKYLPDFPNAAVREKVTIHHLLTHTS
                     GLGSYFDDPEYALDWTRMRHVSDYTTCVASESLAFEPGAEMRYSNSGFIVLGLIIEQV
                     SGRDYYDFVRDTVLRPAGMNDSDSFENDQVVPNLAIGYTMAGTRMRHGGAGASAPAGA
                     SQGGSGSPGAAGGAAQTRKANFYEHSARGTPAGGGYSTAPDLLKFSQALSRGSIVTAA
                     LVDTVTTAKVAMGPGAGYGYGFGDFRGTGDGYFGHNGGAPGMSTDFRIYDKLGYTVIV
                     LSNYDGIAGSVADFIDQLIVPPPTGGPGPGPGGPGPGGTPGRP"
     gene            4344..4898
                     /locus_tag="VF720_10935"
     CDS             4344..4898
                     /locus_tag="VF720_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX7879917.1"
                     /translation="MNPIIPENTARIFTIGHSNRSLADFLELVREFRLGVLVDVRRYP
                     RSRRHPQFNHESLIDAMARLEVRYHHFAELGGYREPLDPASHPALGEPMFKGYAEHMA
                     STEFNVALGRLQARALDARVAVMCAEAQPGECHRSLLSDALVRDGFQVTHLLGSGRTR
                     DHVMSPLVRLDGRRLVYDVGVLSF"
     gene            complement(4969..6243)
                     /locus_tag="VF720_10940"
     CDS             complement(4969..6243)
                     /locus_tag="VF720_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX7879918.1"
                     /translation="MPVVPMLITVGAILGVLAGCGSRADSGPAAGGGAPALTTYHLRA
                     ADLPKPNATPSAVNPARVVARSEGDLLTLPAGFEAFEAAANFRMPRGLALLPGGDIAL
                     SESDADRITVLRDADGDGRFERRWAWLEGVVRPFGIAVAGPFVYVAGTDKIVRCPYAA
                     GDTLVEGEPEFVAWLTPGGYNSHWTRNLLYDAGTNRMFVTVGSKGNVSEDEPIRAAVH
                     VFTVPEDIPEGGVPLTLYASGLRNPVGLSREPVTGIIWTAVNERDGLGDDLVPDYATG
                     LVEGGFYGWPYSYIGKNPQPGSLGTKRPDLVARALVPDVLFESHSAALGIDFYTGTAY
                     PEHYRGGAFVAHHGSWNRSRRTGYKVVYLPFVDGKATGAYEDFMVGFNPDAERSEVWG
                     RPVGVLMDDDGTLLVSDDGGDVVWRVRYTGTR"
     gene            6415..>6740
                     /locus_tag="VF720_10945"
     CDS             6415..>6740
                     /locus_tag="VF720_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX7879919.1"
                     /translation="MSTVDTTPALVEGVYRRMEERLAIVRGRLNRPFTLTEKILFGHL
                     DDPAGQELNAGSSYLMLRPDRVAMQDATAQMALLQFMHAGKDTTAVPSTVHCDHLIQA
                     HVGAEK"
BASE COUNT         1165 a         2313 c         2240 g         1022 t
ORIGIN      
        1 agagtgacgt gcgtcgggag agtgacctgc gccgggagag tgacgtgcgc cgcgagcccg
       61 aggtgcgccg tgagccggaa acccgccgcg agatggaagc ccggcatgag gccgagctcc
      121 gtcgcgaggt ggagccggtc cgcgagcgga tcgagttcac ccggccggtc tcccgcctcg
      181 cggccacgct tgccagcgag tcgtgggccg aggccccgct cgacgcggtg ccgtacgtcg
      241 aacccgatgt cgatatcgag acccgccggc tgaaggatgc ccaggccgtt gccatgcagc
      301 gggtccggga gaatctccgg gaagtcggtg cggctgccca ggagaagtcg gggccggtcg
      361 agctcgatcc ggaggccgtg gccgccggtc acctggacca ggaaccggag cggatcccct
      421 tcgtggaggc ccggctcagc caggcggagg acctcgtccg gacggcggcc cgcgcggccg
      481 tggccaaccg gacgcaggac gaccggtgga agagctccta cacccgcgac aatctcgacg
      541 tgccggcctt catgcggcgg ccgccgacgg agtgagggat ctttgccaac tgaatggacg
      601 ccactgccga gggagtgagc gatgatcgtc ctgatgtgtg aggtgcctgt gatgtaaaca
      661 gtcgggtata caacggaccg accctccggc ccgtctatcg ccccgatgac ccgaacgcga
      721 atccagtcag gaccggtccc ggcctccggg gccggtcctg cccgcctggc ggaccctccg
      781 cccctcctga cggacaaccc cgccctgatt ggctcccgcc gttcccggcc cctggtccgc
      841 gaggacatcc tcgacgccgt cgatgaccag ctcgaacggg tcggttacgc ccggatgacg
      901 atcgaagagg tggcccggct cgccggggtg gggaagggga gcatctacct tcacttcgcg
      961 agcaaggaag acctggcgct ctcgtcgatc gaccggctgg tccggcgact cctcgaccgg
     1021 ctcgccgaaa ttgccgggga accgcttcca gctgccgacc ggctccggcg catgctcgag
     1081 acccgcgtcc tgttccggtt cgatcacgtg aaccaggacg ccgtaagcct cgacgagatg
     1141 ctggcgatcc tccggagcgc gctgctccag cgtcgcgagg agtggttcga acgcgagtcg
     1201 cagctcctgg cgcgggtgat ctccgaggcc cgggcggcgg gcgatgcgaa ggacgccccc
     1261 acgtacgacc acgccctgcg ggacgcccgc gccctcgtca ccgcaaccaa tgcccttctc
     1321 ccgtacagcc tcagcaagtc cgagctcggc cggcgatcgt tgctggtcga ccgcgtgatc
     1381 gccgtggctg atctcctcct ctgcggactg tcgctggatt ccacggcgag cggaccgaac
     1441 catgacccaa ttcgccagga ccgaggtacg aatccatgat gcgacgttcc gcccggccag
     1501 ccacagggcc tctgtcgatg accctgattc tgtctgcact gtttctaacc caggtttccc
     1561 tcgcactacc gccaacccac gcccaggggc cggggtcgat gccggcaccg gtcgatcgcc
     1621 ccacgacggc ggccgccaac ggcgccatcc tcgattcgct cacgacccac ctcaacgcct
     1681 ggtacgtcta cccggagacg gccgcgaaga tggtcaagtc gatcgaatct catcggggca
     1741 aactcgagaa aatcacctcc tcgatcgagc tggccgacgc gttgacggcc gaaatgcagg
     1801 acatcagcca cgacaagcac ctccgggtgg tgtaccgcga gcgggagata ccggtccgcg
     1861 cggtgtcgga ggaattcacg ccggctgaac gggagcggca gcggaaagag agccgcctcc
     1921 gcaatttcgg attcgagaag gtcgagcggt tgatggggaa cgtgggctac ctggagatgc
     1981 gcagcttcgc gcaggtggcg gacggcgcag cggaggccgc cacggcggcc atgaatttcc
     2041 ttggcaacgt cgatgcgctc atcatcgacc tgcggcggaa cggcggcggc agtcccgaga
     2101 tggtggcgct ggtcaccagc tacctctatc cggcaggcga gcggatccac ctgaatgacc
     2161 tgtactggcg gcccggcgat accaccgagc agttctggac ccagccccat gtgccgggcc
     2221 cgcgcctgga tggcaaggac gtctacatcc tgacctcgaa ccgcaccttc tcggcggccg
     2281 aggaattctg ttacaacctc aagaacctgg cgagggccac cattgtcggc gagaacaccg
     2341 gcgggggcgc ccatccgggc acgatggtcc gcctgtccga tcacttcgaa atgttcctgg
     2401 cgaccgggcg ggccatcaac cctgtatcga agacaaactg ggaagggaca ggtgtaccgg
     2461 ccgacatgac ctgccccgcc gaccaggcgc tttcggtggc ccacgtgacg gccatcgaga
     2521 agcttcttgc gaaggcgtcg gatccggacg atcagcagcg attggccatg gcgctcgacg
     2581 cggtgaaggc ccagtcggcc accccggcgg gtggcgaggt ggcgccgccg gccggaccgg
     2641 cacggatcgt ggttcgtcaa aactagacct ggttacctga ggtaattcga aatgacgttg
     2701 gtccccgtga agatgaggcg cgcgcatcgg tgcaaaccca ggcgtctgcc ggtcctgctc
     2761 gtgttcgtcg ttgcgctctc actgttcggc cggttcccgg cgccggcgca agccgcttct
     2821 cccaccatcc ccgatacgcc gatcggcgcc gtcctgagcg agatgctcga cgtcatccgt
     2881 tcgggtgaca tcgagaaggc gcgcgacttc gtgacccgcc gttacgcggc cgaagcgctg
     2941 gcagcacgcc cgatggagag ccgcctcgac gggtggcgtg acctgatggg aagcctcgcg
     3001 cagccgaagc tgctcgaggt gaggacggga tcggggaacc ccgccatgat catcgagtcg
     3061 ggggccaccg gtgaagtctt tgaagtcgtc gtccggccgg cgcccggtgt accggacaag
     3121 atcaccggcg tccggatgca gtcgaccatc cccggcaagc cggccatccc ggccggaccg
     3181 atcaccgaca aggagaggtc ggccctcatc gaccagctgc tcgatcgact ggtggccgac
     3241 gaccgtttct cgggtgtcgt catgctggcg aaggatggca agcctgcgta ccagcgtgcc
     3301 accggcatgg ccaaccagtc ctggagtatt ccgaacagga tcgacaccaa gttctgcctg
     3361 ggctccatga acaagatgtt cacgtcgctg gccgtcgcgc ggctcgtgca ggatggcaag
     3421 ctcgcttacg gcgacaaggt cggcaagtac ctgcccgact tcccgaacgc cgccgtccgc
     3481 gaaaaagtga cgattcatca cctcctcacg cacacctccg gcctcggcag ctacttcgac
     3541 gatcccgagt acgccctcga ctggacgcgg atgcgccatg tgagcgacta cacgacctgc
     3601 gtcgccagcg aatcgctggc cttcgagccc ggtgcggaaa tgcgctacag caactcgggc
     3661 ttcatcgtgc tgggactcat catcgaacag gtctcggggc gcgactatta cgactttgtt
     3721 cgcgatacag tccttcgccc ggccggaatg aacgactccg actccttcga gaacgaccag
     3781 gtcgtgccga acctggcgat cggctacacg atggcgggaa cgaggatgcg gcacggcggg
     3841 gcgggagcat cggcacctgc cggcgcatcg caaggcggct ccggatcgcc cggcgcggcc
     3901 ggcggcgcgg ctcaaacccg caaggccaat ttctacgagc actccgcacg cggcacgccg
     3961 gccggtggcg gctactccac cgcgcccgat ctcctgaaat tcagccaggc gctctccagg
     4021 gggagcatcg ttaccgcggc gctggtcgat acggtgacca cggccaaggt ggcaatgggg
     4081 ccgggcgcgg ggtacggcta cggcttcggc gacttccgcg gcacgggcga cggctacttt
     4141 ggccacaacg gcggggcacc cggcatgagc accgacttcc gcatctatga caagctgggg
     4201 tacacggtga tcgtgctgtc gaactacgac ggcattgccg gcagcgtggc ggacttcatc
     4261 gaccagctga tcgtgccgcc gccaacgggt ggccccggac ctggacccgg tggccccgga
     4321 cccggcggta ccccggggcg gccatgaacc ccatcatccc cgagaacacg gcccggatct
     4381 tcacgatcgg tcattcgaac cggagcctgg ccgacttcct cgagctggtg cgcgaattcc
     4441 ggctgggcgt gctggtcgac gtgcgccgct atccacgaag ccgccgccat ccccagttca
     4501 accacgagtc cctcatcgac gcgatggccc ggctggaggt gcgataccac cactttgccg
     4561 agctcggcgg ctaccgtgag ccgctcgatc cggcgtcaca cccggccctg ggcgaaccga
     4621 tgttcaaggg atacgccgag cacatggcct ccaccgagtt caacgtggcg ctgggccgcc
     4681 ttcaggcccg cgcgctcgat gcccgcgtgg cggtgatgtg cgccgaggcg cagccggggg
     4741 agtgccaccg tagtctttta tccgacgcac tggtgcgcga cggcttccag gtgacgcacc
     4801 tcctcggctc gggacgaacc cgcgaccacg tgatgtcgcc gctggtgcgg ctggatgggc
     4861 ggcggctggt ttatgacgtg ggggtcctga gcttctagcc ccggacaatt ccggaccgcg
     4921 ggtgactccg ccggttggca actcgattcg gaaacgagtt ggccgacctc aacgcgtccc
     4981 cgtgtaccgc acccgccaca ccacatcccc gccgtcatcc gacaccagca gggtgccgtc
     5041 atcgtccatc aggacgccga ccggccggcc ccacacttcc gaacgctcgg cgtcgggatt
     5101 gaacccgacc atgaagtctt cgtacgcgcc agtggccttg ccgtccacga acgggaggta
     5161 caccaccttg tagccggtgc ggcgcgagcg attccacgag ccgtgatgcg ccacgaaggc
     5221 gccgccacga tagtgctcgg gatatgccgt gccggtgtag aagtcgatgc cgagcgccgc
     5281 ggagtgtgat tcgaacagca cgtcgggcac cagtgcccgc gccacgagat cggggcgctt
     5341 cgtgccgagc gaaccaggtt gcggattctt tccgatatag gaatagggcc agccgtagaa
     5401 gccaccttcc acgagcccgg tggcgtagtc gggcaccagg tcgtccccca ggccgtcgcg
     5461 ttcgttgacc gcggtccaga tgatgccggt caccggttcc cgtgagagtc cgaccggatt
     5521 ccggaggccg gaggcataca gcgtgagcgg cacgcctccc tccggaatgt cctcgggcac
     5581 ggtgaacaca tgcaccgcgg cccggatggg ctcgtcctcg gagacgttcc ccttgctgcc
     5641 gacggtcacg aacatccggt tggtgccggc gtcgtagagg agattgcggg tccagtggga
     5701 gttgtagcct cccggcgtca gccaggccac gaactcgggc tccccctcga cgagcgtgtc
     5761 gccggccgca taagggcacc ggacgatctt gtcggtgccg gccacgtaga cgaacggacc
     5821 cgccaccgcg atcccgaacg ggcggaccac accctcgagc caggcccagc gtcgctcgaa
     5881 gcggccgtcg ccgtcggcat cgcgcagcac ggtgatccgg tcggcatccg attcggagag
     5941 ggcgatgtcg ccacccggaa gcagggcgag gccgcgcggc atccggaaat tggcggccgc
     6001 ttcgaatgcc tcgaagccgg cggggagcgt caggagatcc ccctccgacc gggcgacgac
     6061 gcgggccgga ttgacggcgg acggtgtggc gttgggcttc gggaggtccg ccgcgcggag
     6121 gtggtaggtc gtgagggcgg gggcgccgcc cccggcggcc ggtcccgaat cggcccggga
     6181 gccgcagccg gccaggacac cgaggatcgc accaaccgtg atgagcatcg gaacaacagg
     6241 catgaggcag tctagcaggg gagccggcgc acgagcgggg cttcgacgcg gctgacgccc
     6301 cctcaactcc tgcgctatag tatccgggaa attcgcaccc gcccgatgtc cggccccgaa
     6361 ccgcccagtg cagagcgtag tggcgccgga ccccagtgaa aggcagaccc caccatgagc
     6421 acggtcgaca ccacccccgc attggtcgag ggcgtctacc gccggatgga agagcggctc
     6481 gccatcgttc gcggccgcct caaccgcccc ttcacgctga ccgagaagat cctcttcggc
     6541 cacctcgatg atccggccgg ccaggagctc aacgccggct cgagctatct catgctccgg
     6601 cccgatcgcg tggcgatgca ggacgccacc gcgcagatgg cgctcctcca gttcatgcac
     6661 gccggcaagg acacgaccgc ggtgccaagc acggtgcatt gcgaccacct gatccaggcc
     6721 cacgtcggcg ccgagaagga
//
LOCUS       DASVOD010000483         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_293042, whole genome shotgun sequence.
ACCESSION   DASVOD010000483 DASVOD010000000
VERSION     DASVOD010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293042"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1151
                     /locus_tag="VF720_10950"
     CDS             <1..1151
                     /locus_tag="VF720_10950"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEX7879920.1"
                     /translation="VSESFTVPAINIRGMTYDTARAAVRSVQKRDGGAFIFEIARSEI
                     GYTFQRPAEYASQVIAAAVREEFHGPIFIQGDHFQINAKNYATNPDAEVTAVKELIVE
                     AIAAGFYNLDIDASTVVDYGKATLTEQQALNARLTAELTAHVRAHEPEGVTISVGGEI
                     GEVGTQNSTVAELEAFMDAYNAHLATLGSGLAGLSKISVQSGTQHGGVVLPDGSVAEV
                     RIDFDTLQSLSKVAREKYKLAGAVQHGASTLPDDAFDMFPKTGTAEIHLATSFQNLFF
                     DSLYLPDEVKDGMYGWLAANCAGERSADMTDEMFYYRTRKKAYGPFKEAIWKIDWGTR
                     KSLSEDLEDRLDFFFAQLGVDGTQVVVQKKVPHVLVPAPMPEALKAAL"
     gene            1148..1483
                     /locus_tag="VF720_10955"
     CDS             1148..1483
                     /locus_tag="VF720_10955"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX7879921.1"
                     /translation="MTDGAWLFVAPSRDFRSGRPRGFVVAGRPVAVLKLGDAWHAFSN
                     RCPHNGLALEDGGLDGHVLTCRWHGWRFDITTGLSPDVPEGAEGPRIRVYAVRVVDGR
                     VEVTVPPIA"
     gene            complement(1539..>2730)
                     /locus_tag="VF720_10960"
     CDS             complement(1539..>2730)
                     /locus_tag="VF720_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7879922.1"
                     /translation="AVATTELGASTLPRHAGGVKTTPKAGPPFHPSMSQPIRSDPGTR
                     PRRPHHYIIHGAGAVGGTMGALLARAGFQVTLVARPAVVEAIAAQGGLRLIAAGVPGI
                     IAVRAVDRIERVEPAGHPVVCFTMKAGDLAPALPAARAALGTGVAAVTWQNGIRAEHQ
                     AEEHFTDVVGGIVRATSTMLDPGEVRIRTPGILVLGRWPEGRKGVTDDRVETIRADLV
                     TAGFDAVVSPRIAADKGLKLLVNLFSGVSPLVAADGTAMPATARLERNVVIEGARILK
                     AVGIPFHPAGGRGDDVTTMLAHLAGRSVRHRTEDGVHNSTWQNLHHAGRRLENDFMNG
                     EIVSLAAGLGEAAPWNRRLLDLLVEVHGRGLGPNALTDSELAARLTDLADPDPWRPGV
                     DDIP"
BASE COUNT          435 a          914 c          941 g          440 t
ORIGIN      
        1 aggtgtcgga gtcgttcacc gtgccggcca tcaatatccg ggggatgacg tacgacacgg
       61 cacgcgcggc ggtccgctcc gtgcagaagc gcgacggcgg tgccttcatc ttcgagatcg
      121 cccgttcgga gatcggctac acgtttcagc ggccggccga gtatgccagt caggtgatcg
      181 cggccgcggt gcgcgaggaa ttccatggtc ccatcttcat ccagggggac cacttccaga
      241 tcaacgcgaa gaactacgcg acgaatcccg atgccgaggt caccgcggtg aaggaactga
      301 tcgtcgaggc cattgccgcc ggtttctaca acctggacat cgatgcctcc accgtcgtcg
      361 actacggcaa ggccacgctg accgagcagc aggcgcttaa cgcgcggctg accgcggagc
      421 tcacggcgca tgtccgcgca catgaaccgg aaggggtcac gatctcggtg ggtggcgaga
      481 tcggcgaggt cggaacgcag aattccaccg tggccgaact cgaagcgttc atggacgcgt
      541 acaacgccca cctggccacc ctcggatccg ggctggccgg cctctcgaag atttcggtgc
      601 agtcgggaac ccagcatggg ggcgtggtcc ttcctgacgg atcggtggcg gaagtacgca
      661 tcgacttcga cacgctgcag tccctctcga aagtggcccg cgagaaatac aagctggccg
      721 gtgcggtgca gcacggcgct tccacgcttc ccgacgatgc cttcgacatg ttcccgaaga
      781 ccggcacggc ggaaatccac ctggccacgt cgttccagaa cctcttcttc gacagcctct
      841 atctgccgga cgaggtgaag gacgggatgt acggctggct ggccgccaac tgcgccggcg
      901 agcggagcgc cgacatgacc gacgagatgt tctactaccg tacccgcaag aaggcctacg
      961 gccccttcaa ggaagccatc tggaagatcg actggggcac ccgcaagagc ctctccgaag
     1021 acctcgaaga ccggctcgac ttcttcttcg cgcagctcgg ggtggacggt acgcaggtgg
     1081 tggtgcagaa gaaggtgccc cacgttctcg tccccgcacc gatgccggag gccctgaagg
     1141 ccgcgttgtg accgacggcg cctggctgtt cgtggcgccg agccgcgatt tccgttccgg
     1201 gcgcccgcgc ggcttcgtgg tggccggccg gccggtggcg gtgctcaagc tcggtgatgc
     1261 ctggcacgcg ttctcgaatc gctgccctca caatggcctg gcgctcgagg acggcggcct
     1321 cgacggccac gtgctcacct gccgctggca cgggtggcgc ttcgacatca ccacgggact
     1381 gtcaccggat gttcccgagg gggcggaggg gcctcgcatc cgcgtctatg cggttcgggt
     1441 cgtggatggc cgcgttgagg tgacggtccc tccgatcgct taggtcagga gtcgacaagc
     1501 cggcggcgcg ggcgacccat ccgcgcatgg tcggcccgtc agggaatatc gtccacgccg
     1561 ggtcgccagg ggtcggggtc ggcaaggtcc gtgaggcggg ctgccagttc ggaatcggtc
     1621 agtgcgttcg gccccagtcc gcgcccgtgt acctccacga ggagatcaag gagccggcgg
     1681 ttccacggtg ctgcctcgcc aaggccggcg gcgaggctca cgatctcgcc gttcatgaag
     1741 tcgttctcga gccgacgccc ggcgtgatgc aggttctgcc aggtggaatt gtgcacgccg
     1801 tcctcggtgc gatggcgcac gctccgtccg gccaggtgcg cgagcatcgt cgtcacgtcg
     1861 tcgccgcggc caccggccgg gtggaacgga atgccgaccg ctttcaggat gcgcgcccct
     1921 tcgatcacca cgttccgctc caggcgggcg gtcgccggca tggccgtgcc gtcggcggcc
     1981 acgagcggcg atacgccgct gaagagattg accaggagct tcagcccctt gtcggcggcg
     2041 atgcgcggcg agacgacggc atcgaagccg gccgtcacca gatcggcccg gatcgtctcc
     2101 acccggtcgt ccgtgactcc cttccgtccc tcgggccacc gaccgagcac gaggatgccg
     2161 ggcgtgcgga tgcggacttc gcccggatcc agcatcgtgg acgtggcgcg gacaatgccg
     2221 cccacgacat cggtgaagtg ctcttcggcc tggtgctcgg cacgaatgcc gttctgccag
     2281 gtcaccgcgg cgacgccggt tcccagcgcc gcccgcgcgg ccggaagggc cggcgcgaga
     2341 tcgccggcct tcatcgtgaa acagaccacc ggatgtccgg ccggctcgac gcgctcgatc
     2401 cgatcgaccg cccggacggc gatgatgccc ggcacgccgg ccgcgatgag gcggaggcct
     2461 ccctgtgccg cgatcgcttc gaccaccgcc gggcgggcca ccagcgtcac ctggaaaccg
     2521 gcgcgggcga ggagcgcacc catcgtcccg ccgacggcgc cggcgccatg gatgatgtaa
     2581 tggtgcgggc gccgggggcg cgtaccggga tcggaccgga ttggttggct catggatgga
     2641 tggaagggtg gaccggcctt cggcgtcgtc ttgacgcccc cggcatgacg cggtagcgtg
     2701 ctcgctccaa gttccgttgt ggcaacggct
//
LOCUS       DASVOD010000484         1507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_449827, whole genome shotgun sequence.
ACCESSION   DASVOD010000484 DASVOD010000000
VERSION     DASVOD010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449827"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF720_10965"
     CDS             <1..511
                     /locus_tag="VF720_10965"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX7879923.1"
                     /translation="MVSMSEKGREFTQGWFDPGESFGEPPFFSGGVYPATAIAETDCT
                     VWKCPGSGFLRLLEAHPAVHLALTRTLSRRLVYKALMLGEIAIEEAEHRLVTLFGHLA
                     RIAGKQSRADYRIPLTRQQLADMTGLRVETVIRTVKGMEARGVLAIVGGRIVWHGRDD
                     RPESARRKT"
     gene            508..1227
                     /gene="ric"
                     /locus_tag="VF720_10970"
     CDS             508..1227
                     /gene="ric"
                     /locus_tag="VF720_10970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HEX7879924.1"
                     /translation="MTTTTETIRDFVTGDFRTAAVFDKYGLDFCCGGGLPLEDACRKK
                     GIDLDAIRRDLAEITAAPSCGVDDPARWDTGRLIDHIVTKHHAYVRSTLPTIVKHARK
                     VAGVYGAAMPEMVAIADLVELVGTEMASHMDREEMILFPHIRRLAEASAHGYLPPAAP
                     FGSVSSPIAMMEVEHQAAGDEMASIRRLAREYTPPAEACTTFRVLYEELAQFEADLHR
                     HVHLENNILFPRARALEHIEG"
     gene            1232..>1507
                     /locus_tag="VF720_10975"
     CDS             1232..>1507
                     /locus_tag="VF720_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7879925.1"
                     /translation="MPSVAVAGAGLTNPAGARRNLALGTLSFFLCFMAWGLVGAFAPR
                     LRAQFGLSATETALLVAIPVLLGSLARLPMGVLADRFGGRLVFTLLML"
BASE COUNT          240 a          518 c          508 g          241 t
ORIGIN      
        1 catggtctcg atgagcgaga agggacggga gttcacccag gggtggttcg atcccggcga
       61 gagcttcggc gagcccccgt tcttttccgg cggcgtctac ccggccacgg ccatcgccga
      121 gaccgactgc accgtgtgga agtgtcccgg gagcggcttc ctgaggttgc tcgaagccca
      181 cccggccgtg cacctcgcgc tcaccaggac gctcagccga cggctcgtct acaaggcgct
      241 gatgctgggc gaaatcgcca tcgaagaggc ggagcaccgc ctggtcacgc tcttcggcca
      301 cctggcccgg atcgccggga aacagtcgcg cgccgactat cgaattccac tcacccggca
      361 acagctggcg gacatgaccg gcctccgggt ggaaaccgtc atccgaaccg tgaaggggat
      421 ggaggcccgc ggcgtgctcg ccatcgtggg cggacgcatc gtgtggcatg gccgggacga
      481 ccggcccgaa tcagcaagga gaaagacatg acgaccacga ccgaaaccat tcgcgacttt
      541 gtcacgggcg actttcgcac cgcggcggtc ttcgacaagt acgggctcga cttctgctgc
      601 ggcggcggcc tgccgctgga ggatgcctgc cggaagaaag ggatcgacct cgacgcgatc
      661 cggcgcgacc tggccgagat cacggccgcg ccttcctgcg gcgtcgacga cccggcgcgc
      721 tgggacaccg ggcgattgat cgaccacatc gtgacaaagc accacgccta cgtgcgcagc
      781 acgcttccga ccatcgtgaa acacgcccgg aaggtggccg gcgtgtatgg cgccgccatg
      841 cccgaaatgg tggccatcgc cgatctcgtc gagctggtgg gaaccgagat ggcctcccac
      901 atggaccgcg aggagatgat cctcttcccg cacatccggc gcctggccga ggcgtcggcg
      961 cacggctacc ttccgccggc ggcgccgttc ggctcggtga gctcgcccat cgcgatgatg
     1021 gaggtcgaac accaggcggc cggcgacgag atggcttcga tacggcgact cgccagggag
     1081 tacactccgc cggccgaggc ctgtaccacg ttccgcgtcc tgtacgagga gttggcgcag
     1141 ttcgaggcgg atctccatcg ccatgtgcac ctcgagaaca acatcctctt cccgcgggcc
     1201 agggcgctgg aacacatcga ggggtgatgt catgccatcc gtggccgtcg cgggtgcggg
     1261 actgacgaac ccggccggcg cgcgacgcaa tctcgcgctg ggcaccctgt cgttcttcct
     1321 ctgcttcatg gcctggggcc tggtcggcgc gttcgcgccc cggctccggg cgcagttcgg
     1381 cctgtcggcc accgaaaccg cgctgctggt ggccatcccg gtgctgctcg gctcgctggc
     1441 ccggctgccg atgggcgtgc tggccgatcg gttcggcggc cgcctcgtct tcacgctcct
     1501 gatgctg
//
LOCUS       DASVOD010000485         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_240661, whole genome shotgun sequence.
ACCESSION   DASVOD010000485 DASVOD010000000
VERSION     DASVOD010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240661"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1060)
                     /locus_tag="VF720_10980"
     CDS             complement(<1..1060)
                     /locus_tag="VF720_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7879926.1"
                     /translation="MRVALCQINAVIADFEGNRQRIVDWCERAARGGAEVAVFPEMTI
                     TGYPPRDLLERPSFVQRCQQTIRLLTEDSKRWPDLGIVVGAISANPDAGKPLFNEAWV
                     LRGGQIISVHRKTLLPNYDVFDEDRYFEPAPAIALSRLEPEAVGVTICEDIWNDKHSG
                     SGRRYRTNPVETIAEEGARLLVNLSASPYSWGKGRLRQRMLSQIAVEHGFPVVYVNLV
                     GGNDSLIFDGRSLAVAADGTLLAVGAPFEEELLFVDLPEKGRAPVPHPEAEARITLEE
                     MEGTHQALVLGLRDYARKCGFKSVVLGLSGGIDSAVTAVLAADALGAENVHAAFMPTR
                     FTTDLSRQEVEGLARNLGI"
     gene            1340..2116
                     /locus_tag="VF720_10985"
     CDS             1340..2116
                     /locus_tag="VF720_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEX7879927.1"
                     /translation="MYTIDLTGRTGMIFGVANHRSLAWAIAEELGKAGARLAFSYQGE
                     RLKENVAKLAAALPGSEIFECDVTDDAALEKAFAQVGGAFGHLDFLVHSVAFARKEDL
                     EGAFRDTSRDGYRMALEISAYSLLPMARLAAPLMKGRNGSVVALTYLASQRVVPNYNV
                     MGSAKAALEHSVRQLAFELGPMGIRVNAISAGAINTLSARGISGFTSMLSEAREKAPL
                     GRNVTAEEVGKSALFLLSDLGSGVTGETLHVDAGYHVMGV"
     gene            2132..>2637
                     /locus_tag="VF720_10990"
     CDS             2132..>2637
                     /locus_tag="VF720_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879928.1"
                     /translation="MPDTPAGRLRRRTTLRRRVKRWLWREAAFLAALIRHLRWRFLIL
                     LLLLTAGGALFVTLSPEPKPSLVEAVYMTWSLVFGQPPGAFPFHPVLQALFFLVPILG
                     LTIIVEGIIDLARVLRDRTHYERSWCQAMAQTLSDHIILVGLGRLGYRTWQLLNNLDE
                     AVVVIESRA"
BASE COUNT          418 a          938 c          837 g          444 t
ORIGIN      
        1 aaatgccgag gttccgggcc agcccttcga cttcctgtcg cgaaaggtcg gtcgtgaaac
       61 gcgtcggcat gaaggcggcg tgcacattct ccgcaccgag cgcatcggca gccagcaccg
      121 cggtcaccgc cgagtcgatg ccgcccgaga ggccaagcac cacgctcttg aacccgcact
      181 tgcgtgcata gtcgcggagg cccagcacca gggcctggtg cgtcccctcc atctcctcga
      241 gggtgatgcg cgcctcggct tcgggatgcg gcacgggcgc acgtcccttc tccggaaggt
      301 cgacgaacag gagctcttcc tcgaacggcg cgcccacggc gaggagcgtc ccatcggcgg
      361 ccaccgccag gctccgaccg tcgaagatca ggctgtcgtt cccccccacc aggttcacgt
      421 acacgacggg aaagccgtgc tcgacggcga tctggctcag catccgctgc cggagccgtc
      481 cctttcccca cgaatacggt gacgccgaca ggttcaccag aagcctggcc ccttcttcgg
      541 cgatcgtttc gacggggttc gtgcgatacc ggcgcccgct ccccgagtgc ttgtcgttcc
      601 agatgtcctc gcagatcgtc acgcccaccg cctcgggctc gaggcgcgac agggcgatgg
      661 ccggggccgg ctcgaagtaa cggtcctcgt cgaagacgtc gtagttggga agcagtgtct
      721 tgcggtgcac cgaaatgatc tgcccgccgc gaaggaccca tgcctcgttg aagagcggtt
      781 tgcccgcatc cgggttggcg ctgatggcgc cgaccacgat cccgagatcc ggccagcgct
      841 tcgagtcctc cgtaagaagg cggatggtct gctggcagcg ctgcacgaag gagggtcgtt
      901 cgaggagatc gcggggggga taaccggtaa tcgtcatctc ggggaagacc gccacttcgg
      961 cgcccccccg ggccgccctc tcacaccaat cgacaatgcg ctggcggttg ccctcgaaat
     1021 cggcgatcac ggcattgatc tggcacaggg caacgcgcaa agaacctccg gtccattgca
     1081 gcttgggatg cggcgcgact gtcgatttta gcgcctaaac cgtttcggga caacgttttt
     1141 gtcgaaccgg cggcatggtg acagccgagt cgcgcccggt accacccgcg tcggaccacg
     1201 ttcctcgaat tggggaaatt ggcccccgtt cccgagttac gcgcggtttg acgccccgcc
     1261 cccacttcga ggatagtccc cgcgatcgct cccgccggca tggccggcgt gcgaacggga
     1321 ccctggagga ggccaccgca tgtacacgat cgacctgacc ggccggaccg gcatgatctt
     1381 cggtgtggcc aatcaccgga gcctggcctg ggccatcgcc gaggagctcg gcaaggccgg
     1441 cgcccgcctt gccttcagct accaggggga gcgcctcaag gaaaacgtgg ccaagctggc
     1501 tgcagccctc cccggatcgg aaatcttcga gtgcgacgtc accgatgacg cggcgctcga
     1561 gaaggcattc gcccaggtgg gcggtgcctt cggccacctc gatttcctcg tgcacagcgt
     1621 ggcctttgcc cggaaggaag acctcgaggg ggccttccgc gacaccagcc gcgacggcta
     1681 ccggatggcg ctcgagatct ccgcctactc gctcctgccg atggcgcgcc tcgccgcgcc
     1741 gctcatgaaa ggccggaacg gctcggtggt ggccctcacc tacctggcca gccagcgcgt
     1801 cgtgccgaat tacaacgtga tggggagcgc caaggccgcg ctcgagcact cggtgcgcca
     1861 gttggccttc gagctcggcc cgatgggaat ccgcgtcaac gccatctcgg ccggcgcgat
     1921 caacacgctg tcggcccgcg gcatctcggg cttcacctcc atgctctccg aggcgcgcga
     1981 gaaggctccg ctgggccgca acgtcacggc cgaggaagtc ggcaagtcgg ccctgttcct
     2041 gttgtccgac ctgggttcgg gtgtcaccgg cgaaacgctc cacgtggacg ccggctatca
     2101 cgtcatgggc gtgtagcggc gccccgcgcc gatgcccgac acccccgcag gccgactccg
     2161 ccgccgcacc acgctgcgac ggcgcgtgaa gcgctggctc tggcgcgaag ccgcgttcct
     2221 ggccgccctg atccgccacc tccgctggcg cttcctcatc ctcctcctgc tcctcacggc
     2281 cggaggcgcg ctcttcgtga cgctgtcgcc cgagccgaag ccgtcgctgg tcgaggccgt
     2341 gtacatgacg tggagcctcg tcttcggcca gcctccgggg gcgtttccgt tccatccggt
     2401 gctgcaggca ctcttcttcc tcgtcccgat cctcgggctc accatcatcg tcgaggggat
     2461 catcgacctg gcgcgcgtcc tgcgcgaccg aacccactac gaaaggagct ggtgccaggc
     2521 catggcgcag acgctttccg atcacatcat cctggtcggc ctcggccggc tcggttaccg
     2581 cacctggcaa ctcctcaaca atctcgacga ggccgtggtc gtcatcgagt cgcgggc
//
LOCUS       DASVOD010000486         1814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_85152, whole genome shotgun sequence.
ACCESSION   DASVOD010000486 DASVOD010000000
VERSION     DASVOD010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85152"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VF720_10995"
     CDS             complement(<1..1642)
                     /locus_tag="VF720_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX7879929.1"
                     /translation="MPQSRRRPRAFAPGAFGFVRRSLVPLIVAALSVGEAGAGVRRFE
                     VRFEPGQITRAARPFVEGVAWSLPECGVAGEAGAPALPFRPLLLAVPEGERVVSVTAR
                     ASGATIELDLGEPGVGLADGRRDHTSWDGLSSWPAALSRTGPAGWMRGRQLQPVSIWP
                     LSWDPARGVARLTTAITLEMITEPAATPPEVVRPMRPDSPGDRHFAELLEARVLNPED
                     LGGHVVAARDLRPLDSGAAPFSPRFRPSVDGSPVEMVIITTDALAPHFQPLADWKTER
                     GIPTVVRSIEWIRANYPNAVDLGSTLRRFIADAVSRWGSQYVLLAGDAEIVPVRYGKT
                     LYFGGEEIPADMYYQCVDGTWNSDGDAFFGEGYQSSTVTGDGCDLFPDVWLGRAAVSD
                     STDADTFVMKTMTYEKNPAAGYVTRGMIMAEVLFPQHYAQGDSIVYDGATVAEQALAR
                     FPAGFTLARYYENYLPYAGVGAMPEDKPSVVAAVNTGYGVIQHIGHGYVNTMAVGIGG
                     QALGNGDAAGFGNGPRYSLLYSINCTSAALDFNCIGEEW"
BASE COUNT          317 a          616 c          618 g          263 t
ORIGIN      
        1 gccactcctc gccgatgcag ttgaaatcga gggcggccga cgtgcagttg atcgaataga
       61 gaagcgagta acgaggcccg ttgccgaacc cggccgcgtc gccgttgccc agcgcctgcc
      121 cgccgatccc caccgccatc gtgttcacgt agccgtgccc gatgtgctgg atgacgccgt
      181 aaccggtgtt cacggcggca accacggacg gcttgtcctc gggcatcgcc ccgacgcccg
      241 cgtacggcag gtagttctcg tagtagcgtg ccagcgtgaa gcccgccggg aaacgggcca
      301 gggcctgctc ggccacggtg gcgccgtcgt agacgatcga atcgccctgg gcgtagtgct
      361 gcgggaagag cacttccgcc atgatcatgc cgcgagtcac gtagcccgcg gcgggattct
      421 tctcgtaggt catcgtcttc atgacgaacg tgtcggcgtc ggtcgagtcg gaaaccgccg
      481 cccggcccag ccagacatcc gggaagaggt cgcaaccgtc gccggtcacc gtggagctct
      541 ggtaaccctc gccgaagaag gcatcaccgt ccgaattcca ggtgccgtcg acgcactggt
      601 aatacatgtc ggccgggatc tcctccccgc cgaagtacaa cgtcttcccg tagcgaacgg
      661 gcacgatctc ggcgtcgccg gccaggagca cgtactggct cccccaccgg ctcaccgcgt
      721 cggcaatgaa tcgccggagc gtcgagccca ggtcgacggc gttcggataa ttggcccgga
      781 tccactcgat cgagcggacg acggtcggga tgccgcgctc cgtcttccag tcggccagcg
      841 gctggaaatg cggcgccagg gcgtcggtgg tgatgatgac catctcgacc gggctcccat
      901 ccaccgaggg ccggaagcgc gggctgaacg gggcagcgcc gctgtcgagg ggccgcaggt
      961 cccgggcggc cacgacatgc ccgcccaggt cctcgggatt gagcacccgc gcctcgagga
     1021 gctcggcaaa gtgccgatca cccggggaat cggggcgcat cggacgtacc acctcgggcg
     1081 gcgtcgccgc cggttcggtg atcatctcga gagtaatggc cgtcgtgagg cgcgccacgc
     1141 cacgagccgg gtcccaggac agcggccaga tggaaacggg ctgcagctgc cgtcccctca
     1201 tccatccggc cggaccggtg cgggagaggg ccgcaggcca ggagctcagc ccgtcccatg
     1261 acgtgtgatc cctccggcca tccgcgagac cgacccccgg ctcgccgagg tcgagctcga
     1321 tcgtggcacc ggacgcccgg gccgtgaccg agaccacccg ttccccttcg gggaccgcaa
     1381 gaagcaacgg gcggaatgga agcgccgggg cgccggcctc gccggccaca ccgcactcgg
     1441 ggagtgacca ggccacgcct tcgacgaagg gacgcgcagc gcgcgtgatt tgtcccggct
     1501 cgaagcggac ctcgaaccgc cggacaccag ccccggcctc gccgaccgac agggcggcaa
     1561 cgatcagggg aacaagggaa cggcgtacga aaccgaatgc cccgggcgca aatgcgcggg
     1621 gacgtctccg gctctggggc atgctggcct ccttgggcat gtctcggagc ggtccatcga
     1681 actgtcacgt ttcaaaacgc aaaaaaaccc gcgaccggga acctggcggg ctccggtcgg
     1741 ggttctgcgc agtcgttcag ggatcgaaga cggttgggat ccggtcggtt gtgattgtca
     1801 cgcgtgatga aagt
//
LOCUS       DASVOD010000487         2369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_501851, whole genome shotgun sequence.
ACCESSION   DASVOD010000487 DASVOD010000000
VERSION     DASVOD010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501851"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF720_11000"
     CDS             <1..369
                     /locus_tag="VF720_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879930.1"
                     /translation="AWNEAGDVAASGCMVLTITTTQGSDPPTRTVTGERCIGVRCLSD
                     AGSAHQGSQPVGGSIDADGRMSLDLNSGTADNNVFLDAVIEEGDDGWIGGTWVESHFA
                     GPVAHGRFLLDRTAPAAVEP"
     gene            complement(366..755)
                     /locus_tag="VF720_11005"
     CDS             complement(366..755)
                     /locus_tag="VF720_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HEX7879931.1"
                     /translation="MDNDSLGLVGFMMGMGMMMVWCVLLLIVIAAMWVVFQKAGRPGW
                     HSIIPFLNIYDLVQIAGRPGWWMLLLLVPFLNIIVAIIVSIDLARRFGKGTGFGILLA
                     LLPFIGYSILAFSDARYDGGLAPAPIG"
     gene            complement(825..2000)
                     /locus_tag="VF720_11010"
     CDS             complement(825..2000)
                     /locus_tag="VF720_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7879932.1"
                     /translation="MRSIRVFTGLLALFLAIGAADSQAVGLGDLKKKAEKAAKDATKT
                     TTPEAPKAPETGAAGEAAGSASEATAAGGDGGKVSQVSTKYDFVPGDRIILMDDFTRD
                     ELGEFPARWKAWRGNFDVSEFNGERWLRMNGTDGTIAIKADSLPNEWTLEFDFYEKEM
                     TGPVYTVGAFYPGDDSWMWYCAIGGSGNQVAMSAVDGVSSNATTEAPMNGRHHVAVMA
                     KGTGVKVYVDRERLANIAEMDYKGKPMPGAIGLRLWRYGDDPLITNVRFAEGGKEKVD
                     MMATPFVTHGIVFDSGSDKLKPESAPVLRQVGTYLKENPEVKVTITGHTDDVGDTAAN
                     QLLSEKRAAAVAASLAADFGVDAARMTAAGKGESAPIAKNDSPEGKAMNRRVEFAKS"
BASE COUNT          388 a          807 c          764 g          410 t
ORIGIN      
        1 gcctggaacg aggcgggcga tgtggccgcc tcgggctgca tggtcctcac gatcacgacc
       61 acgcaaggct cggatccgcc gacgcgcacc gtgacgggcg agcgctgcat cggcgtgcgc
      121 tgcctgtccg acgcgggaag cgcccatcag gggtcacaac cggtgggcgg ctcgatcgat
      181 gccgacggcc ggatgtctct cgacctcaac tccggcacgg ccgacaacaa cgtcttcctc
      241 gacgcggtga tcgaggaagg agacgacggc tggatcggcg gcacctgggt ggaaagccat
      301 ttcgcgggcc cggtggcgca cgggcgcttc ctgctcgatc gaaccgcccc cgcggccgtc
      361 gagccctaac cgatcggagc gggcgccagt cccccgtcgt accgcgcatc cgagaaggcc
      421 aggatcgagt agccgatgaa cggcaggagc gccagcagga tgccgaagcc ggttcccttg
      481 ccgaagcgcc gggccaggtc gatcgacacg atgatcgcca cgatgatgtt gaggaacggc
      541 accaggagaa ggagcatcca ccagccgggg cgtccggcga tctggacgag gtcgtagatg
      601 ttcaggaacg ggatgatgct gtgccatccc ggccgtccgg ccttctggaa gacgacccac
      661 atcgcggcaa tgacgatgag gaggaggacg caccacacca tcatcatccc catccccatc
      721 atgaatccga ccagacccag ggaatcgttg tccatcgttc ctcccggttg gtgccctgcg
      781 cttacggggc cggacagcct cgcggcccgc atcctactcc tcgatcagct cttcgcgaac
      841 tccacgcggc ggttcatggc cttcccttcg ggcgagtcgt tcttggcgat gggcgccgac
      901 tcgcccttgc cggcggcggt catccgcgcg gcgtccacgc cgaagtcggc cgccaggctg
      961 gcggccaccg cggccgcgcg cttctcggag aggagctggt tggcggcggt gtcgcccacg
     1021 tcgtcggtgt ggccggtgat cgtcaccttc acctcggggt tctccttgag gtaggtgccc
     1081 acctggcgca gcaccggcgc cgactcgggc ttcagcttgt ccgagccgga atcgaagacg
     1141 atgccgtgcg tgacgaaggg cgtggccatc atgtccacct tttccttgcc accctcggcg
     1201 aagcggacgt tggtgatgag gggatcatcg ccataccgcc agaggcgcag gccgatggcc
     1261 cccggcatcg gctttccctt gtagtccatc tcggcgatgt tggccaggcg ttcgcgatcc
     1321 acgtacacct tgacgccggt ccccttggcc atgaccgcca cgtgatgacg gccgttcatc
     1381 ggcgcctccg tcgtggcgtt ggaactcacg ccgtccacgg ccgacatggc cacctggttg
     1441 ccgctgccgc cgatggcgca gtaccacatc cacgagtcgt cgccgggata gaaggccccg
     1501 accgtataga ccggcccggt catctccttc tcgtagaagt cgaactcgag cgtccattcg
     1561 ttcggcagcg aatcggcctt gatggcgatg gtgccgtcgg tgccgttcat ccggagccag
     1621 cgctcgccat tgaactccga cacgtcgaag ttgccgcgcc aggccttcca ccgggccggg
     1681 aattcgccga gctcgtcgcg ggtgaaatca tccatcagga tgatccggtc tcccgggacg
     1741 aagtcgtact tggtcgacac ctggctcacc ttgccgccgt cgccgccggc ggcggtggcc
     1801 tcggaggccg atccggccgc ctcaccggcc gcgccggttt cgggggcctt cggcgcctcg
     1861 ggtgtcgtgg tcttggtggc atccttggcc gccttttccg ccttcttctt cagatcgccc
     1921 agcccaacgg cctgggaatc cgccgccccg atggccagga aaagggccag gagccccgtg
     1981 aacacccgaa ttgacctcat gccagaacct ccctgttgcc accggctccg ccggacgcgt
     2041 tcatcgtgta acgcgaaggc gggggcggaa atggaacatg ctttcgtatt gacatgcatg
     2101 tgcatgcatg ccagcattcg ttatgcgcac cacgatcgaa ttgacagatg agcagcgggc
     2161 acgcctcctg acactggcgg cacgtcgtgg ccagaagggc ttttccatcc taattcagga
     2221 agccgtcgag ctgtacctgg cgcagtcctc caatccggaa acggcgcgtc gtcgagctgc
     2281 ggcacttcgt ggctccatgt cgccggcgga agccgacgaa ctgcgcgagg ccgcccgcaa
     2341 actgcgcgag agctggcgat gatcctggc
//
LOCUS       DASVOD010000488         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_553135, whole genome shotgun sequence.
ACCESSION   DASVOD010000488 DASVOD010000000
VERSION     DASVOD010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553135"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VF720_11015"
     CDS             complement(<1..344)
                     /locus_tag="VF720_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7879933.1"
                     /translation="MPGDALYPRFIMPRLTEALADTPVVLLHGPRQCGKTTLAQVIGH
                     ERGGAYFTFDDAVTLAAATEDPVGFVGDLPERVILDEVQRAPGIFTSLKSAVDRRRQP
                     GRFLLTGSTNVLL"
     gene            complement(496..2289)
                     /locus_tag="VF720_11020"
     CDS             complement(496..2289)
                     /locus_tag="VF720_11020"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879934.1"
                     /translation="MPRSRETPSARLTPARRTTGTSQPAGFLPLFGAAILLSFLIFGA
                     SLNHDWLSDDRPLVQDNLTLQQGNPATVIRIWTSDWWEEIGADGARFRAGDDRNLHRP
                     LTMTTLWFNARLGATTPGPFHVTNILLHALAAALVGALAARRIAFRFAGWISMLVVAL
                     HPVGLDVINRIVGRADILVLAGLAATLLVQTKARDGWTSGRIIVLTLCAIGAMASKES
                     GYAVLPLVVVVAWLDQERTPRPWLAPALVTGATALMLALRVSVVGLPHYVSDPRTDLL
                     ANPLAGLPFVERLPAAVANAAWYVRMLVVPWPMRAIDHPVTLSTWGDPMTWLGALALV
                     AVLTTFGLSLRRRHESSLALGWWLLASLGVGQLLLAIGAFRETRLLYPFLGAFALGIT
                     LIAEDIARSTSRQTARLATRAAALMLPAAWLLIIVNRTAAYTDDRHLLEADLKHHPAP
                     LTWLQLGTLYMESGRMAEARTAKENALTLAPNSTQALADVGAFHVDTDNFARGDSLLT
                     RSLAIAPQNSVALLNLGTLRLRQNRLEESHDLLTRAEALDPDFILTQLNLTLLEMRMG
                     RTDSVRKRLDKLRVQAPGDPRVADLMRGLGP"
     gene            2455..>3344
                     /locus_tag="VF720_11025"
     CDS             2455..>3344
                     /locus_tag="VF720_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HEX7879935.1"
                     /translation="MSSTPRDEARLQVNSRKPRVFDPPRLPDGRRSPISAYFGDHVLD
                     LIKLKEKLPREIYSSLAGTLKNSHPLTKEVADTIASVARDWATQHGATHFTHWFQPMT
                     GSTAEKHDSFIDLNVSMTGELKVLEKLSGGQLVQSEPDASSFPSGGMRSTFEARGYTA
                     WDPSSPMFLVESTNGMTLCIPSVFISYFGDSLDHKTPLLRSLEVLSKNATAFLKLLGD
                     VDVHAVTATLGCEQEYFLVDRTHFALRPDLMLTERTLLGAASPRGQQFEDHYFGSIPS
                     RVLAFMEEMEFECHRLGIPIR"
BASE COUNT          587 a         1054 c         1162 g          541 t
ORIGIN      
        1 agcagcacgt tcgtcgagcc ggtgagcagg aaccggccgg gttgccgccg ccggtcgacc
       61 gcggacttga gcgaagtgaa gatgcccggc gcgcgttgca cctcgtcaag gatcacgcgc
      121 tccggcagat cccccacgaa cccaaccggg tcctccgtgg cggcggcgag tgtcacggcg
      181 tcatcaaacg tgaaataggc gccgccgcgc tcgtggccga tgacttgcgc gagcgtcgtc
      241 ttgccacact gccggggccc gtgaaggagg accaccggcg tgtcggccag cgcctccgtg
      301 aggcgaggca tgatgaatct cggataaagg gcgtcaccgg gcatgctggc taatcgtaac
      361 ctttgccttc gaccaattgc aaggttttgt gttggctatc tgtaaccctc agggggtgag
      421 gaagaagctg gtcgtcaagt cgcaggagaa tgcaattctt ggataattca ttattttaca
      481 attggttaaa tcaattcacg gccccaaccc cctcatgaga tccgccaccc gcggatcccc
      541 cggcgcctgc accctcagtt tgtccagccg cttccgcacc gagtcggttc gccccatgcg
      601 catctcgagc agggtcaggt tgagctgcgt gaggatgaag tcggggtcga gggcctcggc
      661 gcgggtgagc aggtcgtgcg attcctcgag gcggttctgg cggaggcgaa gtgtgccgag
      721 gttcaggagc gccacgctgt tctgtggcgc gatcgccagg gatcgagtca gcaacgagtc
      781 gccgcgagcg aagttgtcgg tgtcgacatg gaaggccccg acgtccgcca gggcctgcgt
      841 cgagttcggc gccagcgtga gcgcattttc cttggcggtg cgggcttcgg ccatgcgacc
      901 ggattccatg tacagggtgc cgagctggag ccacgtgagt ggggcggggt ggtgcttgag
      961 gtcggcttcg aggaggtggc ggtcgtcggt gtaggcggcg gtgcggttga cgataatgag
     1021 gagccaggcg gcggggagca tcagcgccgc ggcccgcgtc gccagccggg cggtttgacg
     1081 gctggtcgat cgcgcgatgt cctcggcgat gagggtgatc ccgagcgcga aagccccgag
     1141 gaagggatag aggaggcgcg tttcgcgaaa cgcgccgatg gccaggagga gctggccgac
     1201 gcccagggag gccaggagcc accatcccaa cgcaagggac gactcatggc gacgtcggag
     1261 tgatagtcca aaggttgtga gcacggcgac cagcgccagg gcgccgagcc acgtcatcgg
     1321 gtcgccccag gtcgacagcg tcaccgggtg atcaattgcg cgcatcggcc acggcacgac
     1381 cagcatccgg acataccacg cggcgttggc cacggcggcg ggaaggcgct cgacgaacgg
     1441 gaggccggcc aggggattgg cgagcaggtc ggtgcgcgga tcggaaacgt agtgcggcag
     1501 gccgacgacc gacacgcgca gggccagcat caacgccgtg gcgcccgtta ccagcgccgg
     1561 cgcgagccac ggccgcggcg tcctctcctg gtcgagccac gccacgacga caacgagtgg
     1621 caggacggcg tacccggact ccttgctcgc catcgcgccg atcgcgcaca gcgtcaggac
     1681 gatgattcga ccgctcgtcc agccatcgcg cgccttcgtc tgcacgagga gggtggcggc
     1741 cagtccggcc aggacaagga tgtcggccct gccgacgatc cgattgatga catcaagccc
     1801 gacgggatgg agtgccacga cgagcatgga gatccatccg gcaaatcgaa acgcgatccg
     1861 gcgcgcggcc agcgcgccga ccaatgcggc ggcgagggcg tgcagcagga tgttggtgac
     1921 gtggaagggg ccgggcgtcg tcgcgccgag tcgtgcgttg aaccacaggg tcgtcatcgt
     1981 cagcgggcgg tggagattcc ggtcatcgcc ggcacggaag cgggccccgt ccgcgccaat
     2041 ctcctcccac cagtcactgg tccaaatccg gatgacagtc gccggatttc cctgctgcag
     2101 cgtcaggttg tcctgcacca gcggccggtc gtcgctcagc cagtcatggt tgagcgaggc
     2161 gccgaagatg aggaacgaca ggaggatggc ggcgccgaac agggggagga aacccgcggg
     2221 ttgcgaagtg cccgtcgtgc gtcgcgcggg ggtcagccgt gcggagggcg tctcacggga
     2281 acgcggcatc ggccgatact aacaagaatt gctccgcgga cggtccgggc tcgtgcggta
     2341 tactctccgg cattcattgg ggattccgcg tggacggtcg ttcgcgggag ttcccgtttc
     2401 ccggtgatcg cggcaggctt cggctggacg tggttgccac ggaggagatg acgcatgagt
     2461 tccacccccc gcgatgaagc gcgccttcag gtcaattccc gcaagccgcg cgtcttcgat
     2521 ccgccccgac ttcccgacgg ccgccgttcg ccgatcagcg cctacttcgg ggaccacgtc
     2581 ctcgacctga tcaagctgaa agagaagctg ccgcgcgaga tctactcgag cctggcgggc
     2641 acgctcaaga acagccaccc tctcaccaag gaagtggccg acacgatcgc ctcggtggcg
     2701 cgcgactggg ccacgcagca cggcgccacc cacttcacgc actggttcca gccgatgacg
     2761 ggttccaccg ccgagaagca tgacagcttc atcgacctga acgtgtcgat gaccggcgag
     2821 ctgaaggtgc tggagaagct ctcgggcggc cagctcgtgc agagcgaacc cgacgccagc
     2881 tcgttcccgt cgggcggcat gcgctccacg ttcgaggcgc gcggctacac ggcgtgggat
     2941 ccctcctcgc cgatgttcct cgtggaatcg accaacggca tgacgctctg catcccgtcg
     3001 gtcttcatca gctatttcgg cgacagcctc gaccacaaga cgccgctgct gcggagcctc
     3061 gaggtcctct cgaagaacgc cacggccttc ttgaagctgc ttggggatgt ggacgtgcac
     3121 gccgtcaccg ccacgctcgg ctgcgagcag gaatatttcc tggttgaccg gacgcacttt
     3181 gccttgcggc cggacctgat gctgacggag cgcacgttgc tcggcgcggc ctcgccgcgc
     3241 ggccagcagt tcgaggacca ctacttcggc tcgatcccga gccgggtgct ggcgttcatg
     3301 gaagagatgg agttcgagtg ccaccggctc gggatcccga tccg
//
LOCUS       DASVOD010000489         4334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_657359, whole genome shotgun sequence.
ACCESSION   DASVOD010000489 DASVOD010000000
VERSION     DASVOD010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657359"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /locus_tag="VF720_11030"
     CDS             <1..1566
                     /locus_tag="VF720_11030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7879936.1"
                     /translation="IRTKADFQGKSQTAVMATLRKENVEVFLVQDRPALMTAIADLYR
                     LSAIGVRARWRFLLSADMKGGQLASPFTLIDVGLRDLPGMVARIGREAALGGFEVLRS
                     HLPLRARRKARVLGPGPSYRIAYLRSDLSFVGLGAGGSVSHTLGVINGLREAGHEVDI
                     IAADAIPGLESSGLHQTIISPRGEIRIHDEAAMLAYHHHFVAEAQQVIKSRKPDFLYQ
                     RHAVFNATGAVLANIFDIPLLLEANHTEVGAKAMWSRLQFRRLGEQFERAAFDSADAI
                     VAVSAIGAAALAKVGADPDKILVNPNGVDPKVFRPDIDGTALRARLGFRHDDVVAGFL
                     GTFTRWHGVLFLAEQVPALVPRHPDARFLFMGDGDLRSAVEDQLKRAGVADRAVFTGL
                     IPHHAVPEHLAACDVLISPHLPFEDGTPFFGSPTKLFEYMAMGRAVVASNLGQIGDVI
                     EDRKSGMLYGPGMAGEFVEAMDRVLADGAERALLGQNARRRVETEYTWRRNAERAVAF
                     LAERVRAGHPGRL"
     gene            1586..2545
                     /locus_tag="VF720_11035"
     CDS             1586..2545
                     /locus_tag="VF720_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7879937.1"
                     /translation="MSERERVIIFGGAGFIGSHLVEDLLAHGHFVRAFDKTRGDWRNL
                     AAVADRVETVEGDFVNRVEVRQALDGCTTAIHLVSATLPSSSNENPAYDIESNVISTL
                     NLLDECREAGVKRVLFISSGGTVYGPAHRLPIPEDHPTEPACSYGIGKLAIEKYLALY
                     GRLYGLSSAVLRFSNPFGERQNPAAAQGAVAVFLGRVHRGEPIDLWGDGSAVRDYLYI
                     TDGVRAFRRVLETPDASGVFNVGRGVGTSLTELIDAIGRVTGRKVIVNRLPGRPLDVP
                     ANVLDTSRLTALTGWRAEVDLEEGLARTWAWMTGDEPVAVGTP"
     gene            2542..3345
                     /locus_tag="VF720_11040"
     CDS             2542..3345
                     /locus_tag="VF720_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7879938.1"
                     /translation="MSIDPKTFSLSVFFPAYYDEKNIGKVTEGVLKAVREMGIETYEI
                     TIVEDGSPDRTGEVADELAAKHPNVRVIHHPKNLGYGAALKTGFTSARHEWVFYTDGD
                     NQFDLSELKKFVALTPYTDIVAGFRINKQYSAWRKFTSFIYNLVLRVLFDIRDRDVDC
                     AFKIYRRDLFDKIVIESNDAFIDAEIAIKARLLNYSSTEVGVTHLPRVDGVSTGARPS
                     VILRTIREIRNYHRRYRRELSRLKAGESPGPELLGRPAPASSPTRQETA"
     gene            3345..>4334
                     /locus_tag="VF720_11045"
     CDS             3345..>4334
                     /locus_tag="VF720_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX7879939.1"
                     /translation="MTRALVRSLIHAALPLVILVSLNPATAPARETPEARPTPPTPGI
                     YRLGELHRVEAARLDRSHCFLLQALSPLDLHGPHLPAATSFELSDAFTDAVAERLRKS
                     KPEWSVVILPPLPIGAGSTGELGGVYLHSSTVPVDLFMLRRFLGNWFLAPAENAHPNL
                     SIISFHLDPQHLKAISDACDYYVQNYGMGAINVMSYLMADSTTLAGARAIGTKALGDV
                     PEADLVFEMLSGGVAETSMMLHVHPDEVDRDFTELEPRIATSWGDAVTIIKTFGWPGY
                     LGHPARATPAYGKSLWEYLADRAAALVLERAEGSLMTARPRFEDAIRTDFALRE"
BASE COUNT          710 a         1502 c         1391 g          731 t
ORIGIN      
        1 atcaggacga aggccgactt ccaggggaag tcgcagacgg cggtgatggc gaccttgcgg
       61 aaggaaaacg tggaagtgtt cctggtacag gaccgacccg cgctgatgac ggccattgcc
      121 gacctctatc gactgtcggc catcggcgtt cgagccaggt ggcggttcct cctgtcggcc
      181 gacatgaagg gcggacagtt ggcgtcgccg ttcacgctga tcgatgtcgg cctcagagac
      241 ctgcccggca tggtcgcccg gattggccgc gaggccgccc tgggaggctt cgaggtactc
      301 cgcagtcacc ttccgcttcg cgcgaggcgg aaggcgcggg tactcgggcc cggtccttcc
      361 taccggatcg cctacctccg gagtgacctg tcgttcgtcg gcctcggagc gggtggatcg
      421 gtatcccaca cgcttggcgt catcaatgga ctgcgcgaag cggggcacga ggtcgacatc
      481 attgccgccg atgccattcc gggcctcgag tcgagcgggc tgcatcaaac gatcatttcg
      541 ccgcgaggcg aaatccgcat tcatgacgaa gcggcgatgc tggcgtatca ccaccacttc
      601 gttgccgagg cacagcaagt catcaagtcc cggaagcccg actttctcta ccagcggcat
      661 gccgtcttca acgccaccgg cgccgtcctg gccaacatct ttgacattcc gctcctcctc
      721 gaagccaatc acaccgaggt cggcgccaag gccatgtgga gccgcctgca gttccggcgg
      781 ttgggcgagc agttcgaaag agcggcattc gactcggcgg acgccatcgt ggccgtctcg
      841 gccatcggcg cggcggcact ggcgaaagtg ggggccgatc ccgacaagat cctggtgaat
      901 cccaatggcg tcgatccgaa ggtcttccgg ccggatatcg acggcacggc cttgcgcgcg
      961 cgactcgggt tccgccacga cgatgtcgtg gcgggattcc tcggcacgtt cacccgctgg
     1021 cacggtgtcc tcttcctggc ggagcaggtg ccggcgctcg tgccccggca ccccgatgcc
     1081 cgcttcctct tcatggggga tggagacctc cgctccgcgg tggaggatca gctgaaacgg
     1141 gccggcgtgg ccgaccgggc cgtcttcacc ggcctcatcc cgcaccacgc cgtgccggaa
     1201 catctggcgg cgtgcgatgt tctgatctcg ccccacctgc cgttcgagga cggcaccccc
     1261 tttttcggct ccccgaccaa gctgttcgaa tacatggcga tggggagggc ggtggtggcc
     1321 tcgaacctcg ggcagatcgg cgacgtgatc gaggaccgaa agagcggtat gctctacggc
     1381 cccggaatgg ccggggagtt cgtggaggcc atggaccggg tgctggccga cggcgcggaa
     1441 agggccctcc tggggcaaaa cgcccgccgg cgggtcgaga ccgagtacac ctggcggcgg
     1501 aacgccgagc gggcggtggc cttcctggcg gagcgggtcc gcgcggggca tccgggccgg
     1561 ctatgacaca tttcggggag agagcttgag cgaacgcgag cgggtgatca tcttcggcgg
     1621 ggcgggcttc atcggcagcc atctcgtcga ggatctgctg gcccacgggc acttcgtgag
     1681 ggccttcgac aagacccgtg gcgactggcg caacctggcc gccgtggccg atcgcgtgga
     1741 aaccgtcgag ggagacttcg tcaaccgcgt ggaggtccgc caggcgctcg acggatgcac
     1801 cacggccatc cacctcgtgt cggccaccct gccgtcgagc agcaacgaga accccgccta
     1861 cgacatcgag tccaacgtca tctccacgct gaacctcctg gatgaatgtc gcgaggccgg
     1921 cgtgaagcgg gtgctcttca tctcctcggg cggcacggtc tacgggccgg cccaccggct
     1981 tccgattccc gaggatcatc ccaccgaacc ggcctgctcc tacggcatcg gcaagctggc
     2041 catcgagaaa tacctggcgt tgtacggccg tctctacggc ctctcgagtg ccgtgctccg
     2101 cttctcgaat ccgttcgggg agcgccagaa tcccgcggcg gcccaggggg ccgtggccgt
     2161 gttcctcggg cgcgtgcacc ggggcgaacc gatcgacctg tggggtgacg gctcggcggt
     2221 ccgcgactat ctctacatca ccgacggcgt gcgcgccttc cgccgcgtcc tcgaaacgcc
     2281 cgacgcctcg ggcgtcttca acgtgggacg aggcgtcggg acgagcctca cggaactcat
     2341 cgacgccatc ggccgcgtga ccggccggaa agtgatcgtg aaccgcctcc cgggccgtcc
     2401 gctcgatgtg cccgccaacg tgctcgacac ctcgcgcctc acggcgctga ccggctggcg
     2461 ggcggaggtg gacctcgagg aagggttggc ccgcacctgg gcctggatga ccggcgatga
     2521 gccggtggcc gtgggcaccc catgagcatc gatccgaaga cgttttccct ctcggtcttc
     2581 ttcccggcct attacgacga gaagaacatc ggcaaggtca ccgaaggggt gctcaaggcc
     2641 gtgcgcgaga tggggatcga gacgtacgag atcaccatcg tggaggacgg gagccccgac
     2701 cggacaggtg aggtggccga tgagctggcg gcgaagcacc ccaacgtgcg cgtcatccac
     2761 catccgaaga acctcggtta cggcgcggcc ctcaagaccg gtttcaccag cgcccgccac
     2821 gagtgggtgt tctatacaga cggcgacaac cagttcgacc tgtcggagct caagaaattc
     2881 gtggcgctca cgccctacac cgacatcgtg gccggattcc gcatcaacaa gcagtactcc
     2941 gcctggcgga agttcacctc gttcatctac aacctcgtgc ttcgcgtact gttcgacatc
     3001 cgcgaccgcg acgtggactg cgcattcaag atctaccgtc gcgacctctt cgacaagatc
     3061 gtcatcgaga gcaacgacgc cttcatcgac gccgagatcg ccatcaaggc ccggctcctc
     3121 aactattcgt cgacggaagt gggcgtgacg cacctgccgc gcgtggacgg cgtttccacg
     3181 ggggcgcggc cgagcgtcat cctgcgcacc atccgcgaga tccggaacta tcaccgccgc
     3241 tatcgccgcg agctctcccg tctgaaggcg ggggaatcgc ccggccccga gctcctcggc
     3301 cggccggcgc cggcgtcgtc gcccacccga caggaaaccg cttgatgacc cgcgcgctcg
     3361 tccgctcctt gatccacgcc gcactcccgc tggtgattct cgtttcgctg aaccccgcta
     3421 cggcccccgc ccgcgaaacg cccgaggccc gcccgacgcc ccccacgccc ggcatctacc
     3481 ggctgggcga gcttcaccgg gtcgaagcgg cccggctcga ccgcagccac tgttttctcc
     3541 tgcaggcgct ctcgccgctt gacctgcacg gacctcatct tcctgccgcc acgtcgttcg
     3601 aactgagcga tgcattcacg gatgccgtgg ccgagcggct gcgcaagtcg aagccggagt
     3661 ggtcggtcgt gattttgcct cccctgccga tcggcgccgg ttccaccggg gaactgggcg
     3721 gcgtgtacct tcactcatcc accgtgcccg tcgatctctt catgcttcgc cggtttctcg
     3781 gaaactggtt cctggctccg gccgagaatg cccaccccaa tctctcgatc atcagtttcc
     3841 atctcgatcc ccagcacctg aaggccatct ccgatgcctg cgactactac gtgcagaatt
     3901 acggcatggg ggccatcaac gtgatgagct acctgatggc cgactccacc acgctggccg
     3961 gcgcgcgcgc catcggcacg aaggcgctgg gtgacgtccc ggaggcggac ctcgtcttcg
     4021 agatgctctc ggggggtgtt gcggaaactt cgatgatgct ccacgtgcat cccgacgaag
     4081 tggacaggga cttcacggaa ctggagccgc gcatcgccac gtcgtggggc gacgcggtca
     4141 ccatcatcaa gacgttcggc tggccgggct acctcggcca ccccgcccgc gccacgcccg
     4201 cctacggcaa gtcgctctgg gaatacctgg ccgaccgcgc ggccgccctg gtcctggagc
     4261 gggcggaggg ctcgctcatg accgcccgcc cccgcttcga ggacgccatc cggaccgact
     4321 tcgcgctccg cgag
//
LOCUS       DASVOD010000490         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_972222, whole genome shotgun sequence.
ACCESSION   DASVOD010000490 DASVOD010000000
VERSION     DASVOD010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972222"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..191)
                     /gene="ssrS"
                     /locus_tag="VF720_11050"
     ncRNA           complement(7..191)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VF720_11050"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(245..541)
                     /locus_tag="VF720_11055"
     CDS             complement(245..541)
                     /locus_tag="VF720_11055"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEX7879940.1"
                     /translation="MNRPITETNVTIRGVAYQIRGDVDPDHLERLAEHVDGTMKVLEG
                     SSAAQSPAKLAIVASLTIADELFQEREARTADWTEIRGRITKLETMLDEALSSE"
     gene            complement(656..961)
                     /locus_tag="VF720_11060"
     CDS             complement(656..961)
                     /locus_tag="VF720_11060"
                     /inference="COORDINATES: protein motif:HMM:NF017786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HEX7879941.1"
                     /translation="MTDRMDDIEPILEALEARVARAVTRIRELSAENRRLKASGSAAT
                     PAVTGITNGSDTAALEERIRELDRERVALLADRRALTQRVEQIIARLEYLESETVTH"
     gene            complement(958..>1980)
                     /locus_tag="VF720_11065"
     CDS             complement(958..>1980)
                     /locus_tag="VF720_11065"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879942.1"
                     /translation="VEEVARFHGYDTLSEREWNASGLAAKRAPLESWITRIREAWVAL
                     GFSEMKNPVLSDPEMHRKAGIAEDEMTARGVHIVDPQSTEQSLMRLSLLPAALGAIAR
                     NQRHGRSEVRLFEIGATYRWAETGPLADEPLELLVVATGGDFGPDLTRLDPVMDELRF
                     KGLVEAFLARLRIDTPEWRCYDATGLINGTSAIITSGNTRLGDVGTLSGDRTTAFGID
                     RPVYRAHFDVAALLASRREVVRFREVSRFPASRRDLAFLVDRTVDEGAVREAIRKLGG
                     ALLVEAVLFDRFEGKPLPEGKISLAYALRFRADDRTLSDEDVRRAAGEIAEGLAKSLG
                     AELRDG"
BASE COUNT          328 a          679 c          613 g          360 t
ORIGIN      
        1 gcaaaaaaat tccccgccga ctgccgtcag gccgggaacc ttgaacctgc aaaacaagtg
       61 ggcacctaac ttccggcgtt gcgcccccga gatcgcaaga tgtcgggctc cgctatccgg
      121 aagacttggc ccccgttcca caagtacgtt ggctcaaaat taccgggcca tcacgtgcag
      181 ggcagggaaa gagcgtttcg ggctcgagcg tcgtcgttac tgggaccgac gccgcggatg
      241 attgtcactc actcgagagc gcttcgtcca gcatcgtttc gagttttgtg atccgtccgc
      301 ggatttcggt ccagtctgcc gtgcgggcct cccgttcctg gaagagctcg tcggcaatgg
      361 tcaacgacgc cacgatggcc agcttggcgg gtgactgggc ggcgcttgag ccttcgagca
      421 ccttcatggt gccgtcgaca tgctctgcca ggcgttccag gtgatcgggg tccacatctc
      481 cccggatctg gtacgccaca ccccttatgg tcacgttggt ctcagtgatc ggtcggttca
      541 tgaggtcaat acctccacgt ccgcttcacc gtgccgctgg cgatcgattc cttggattct
      601 tcccacccct gcggaacgga cgcgagtccg tccccatcta tgtgaaagcg gtgcgctaat
      661 gcgtcaccgt ctccgattcc aggtattcca gccgcgcgat gatctgctcc acccgctgag
      721 tcaacgcgcg acgatccgcc aggagcgcca cccgttcccg gtcgagctcc ctgatccgtt
      781 cctcgagcgc cgccgtgtcc gaaccgttcg tgattccggt caccgccggg gtagcggcac
      841 tgccggaagc cttgagccgc cgattctcgg ccgacaactc ccggatccgg gtgaccgcgc
      901 gcgccacgcg tgcctcgagg gcttcaagga tcggttcgat gtcgtccatg cgatcggtca
      961 tccgtccctc aactcggcgc ccagcgactt cgcgagcccc tcggcaatct ccccggccgc
     1021 ccgacgcacg tcctcgtcgg aaagggtccg gtcgtcggcc cggaagcgca acgcgtacgc
     1081 caggctgatc tttccttccg gcagcggctt cccttcgaat cggtcgaaga gcaccgcctc
     1141 cacgaggagc gcgccaccca gcttgcggat ggcctcgcgg accgccccct catcgaccgt
     1201 tcggtcgacc aggaaggcca ggtcgcgtcg ggaagccggg aagcgcgaga cctcccggaa
     1261 cctcaccacc tcgcgacggc ttgccaggag cgcggccacg tcgaaatgcg cccggtaaac
     1321 cggtcggtcg atgccgaacg cggtcgttcg atccccggac agggtcccga cgtcgccgag
     1381 gcgtgtgttg ccactcgtga taatcgccga agtgccgttg attaaaccag ttgcatcgta
     1441 gcaacgccat tcgggggtgt caatccggag acgcgccagg aaggcctcca cgagcccctt
     1501 gaagcgcaac tcgtccatta cgggatcgag acgggtgagg tcgggaccga agtcaccccc
     1561 cgtggccacg acgaggagtt cgagaggctc atcggccaat ggacccgttt cggcccagcg
     1621 gtaggtggcg ccgatctcga agaggcgcac ctcgctccgg ccgtgccgct ggttccgcgc
     1681 gatggcgccg agcgccgcgg ggaggagcga gaggcgcatc aggctctgct cggtggattg
     1741 cgggtcgacg atgtgcacac cacgggcggt catctcgtcc tcggcgattc ccgccttgcg
     1801 atgcatctcc gggtccgaga gtaccgggtt cttcatctcg gagaagccga gcgccaccca
     1861 ggcctcgcgg atgcgcgtga tccagctctc gaggggcgcg cgcttcgcgg cgaggcccga
     1921 ggcgttccat tcccgctcgc tcaacgtgtc ataaccgtgg aagcgcgcca cttcctcgac
//
LOCUS       DASVOD010000491         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_866778, whole genome shotgun sequence.
ACCESSION   DASVOD010000491 DASVOD010000000
VERSION     DASVOD010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866778"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VF720_11070"
     CDS             complement(<1..1254)
                     /locus_tag="VF720_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879943.1"
                     /translation="MPAILRSTVVLCVLLALGARQADVVPQKLRTSGARKALDAWSRA
                     RAFPDEFIPSDGYSRGVAQAEAMPAAVAMLDGRSPDRGDLDPASHLSGPKWRSLGPNN
                     IGGRTLCVTLNPLRPSTVYAGSAAGGLWISRTGGAGTNAWKRVVTGHPVLSASCLAIS
                     TRDTSVMYLGTGEVYAWQNTLGGLIGRGTRGSYGMGILRSTDAGRTWSTSLDWSRDQK
                     RGVWAIRVDPEEPNRVWAATTEGVYRSIDSGAHWSLSLNVVMATDIELDPQNPDTAFA
                     ACGDFNSPGYGIYRTFNGGANWTKLGGGLPAVWGGKAQIVVSPTFTNVVYASIGGMGA
                     SVVGTNVMRSFDSGTTWTTMSSTVDYSTYQYWYSHDIAVQPNNFENVWVCGIDVWKST
                     TSGSNLARVSDWQLAYLGVVPVGGPE"
     gene            1400..>1852
                     /gene="mraW"
                     /locus_tag="VF720_11075"
     CDS             1400..>1852
                     /gene="mraW"
                     /locus_tag="VF720_11075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX7879944.1"
                     /translation="MTDAGTPKPARRPRYRGTHPRTFDQRYKEKDPARFPTMEAHIRA
                     QGRTPAGSHVPVMLDESIDLLALAPGNVVVDATLGRGGHASAILDAIAPGGRLIGVDH
                     DQAELDRTRERFRRERPGAPVTYVHSTFAGLGKIHAEHAPEGFDAVLAD"
BASE COUNT          302 a          654 c          629 g          267 t
ORIGIN      
        1 ctcgggcccc ccgaccggca ccaccccgag gtaggcgagc tgccagtccg acaccctggc
       61 caggttcgag cccgaggtgg tcgacttcca cacgtcgatg ccgcacaccc agacattctc
      121 gaagttgttg ggctggacgg cgatgtcgtg cgagtaccag tactggtagg tcgagtagtc
      181 gacggtggac gacatggtcg tccacgtcgt gccgctgtcg aacgaacgca tcacgttcgt
      241 gccgaccacc gaggccccca tccccccgat cgacgcgtac accacattcg tgaacgtggg
      301 cgacaccacg atctgcgcct tgccgcccca cacggcgggg agcccgcccc cgagctttgt
      361 ccagttggcc ccgccattga acgtgcggta gatgccgtaa cccgggctgt tgaagtcacc
      421 gcaggccgca aaggcggtgt cgggattctg cggatcgagc tcgatgtcgg tggccatcac
      481 cacgttcagg ctcagcgacc agtgcgcgcc ggagtcgatc gagcggtaca ccccttcggt
      541 cgtggccgcc cacacccggt tcggttcctc gggatcgacg cggatggccc acacgccgcg
      601 cttctggtcg cgtgaccagt cgaggctcgt cgaccaggtc ctcccggcgt cggtggagcg
      661 gagaatcccc atgccgtagc tgccgcgcgt tccgcggccg atcaacccgc caagcgtgtt
      721 ctgccaggcg tacacctcgc cggtgccgag atacatcacg ctggtgtcgc gcgtggaaat
      781 ggccaggcaa ctggccgaga ggacggggtg gccggtcacc acgcgcttcc acgcattcgt
      841 ccccgcgccg ccggtccggc tgatccagag cccgccggcc gccgacccgg catacaccgt
      901 ggaggggcga agcgggttga gcgtgacgca gagggtgcgt ccgccgatgt tgttcgggcc
      961 cagtgagcgc cacttcgggc cggaaaggtg ggatgccgga tcgagatcgc cccgatcggg
     1021 tgaacggcca tcgagcatcg caaccgcggc cggcatcgcc tccgcctggg caacgccgcg
     1081 tgagtagcca tccgagggga tgaactcgtc gggaaaggca cgcgcccggc tccaggcatc
     1141 gagcgccttg cgggcaccgg aggtgcgcaa cttctgcggg acgacgtcgg cctgccgggc
     1201 gccgagggcg agcaggacac aaaggacgac ggtgctgcgg aggatggcgg gcatgtcttt
     1261 cccctcggtt cttcgggctc gttggtggtg gggctcgcgg cgccgcagcc ggccgccggc
     1321 cgtccttgcc ggggaggggg cccgggacgg acaagggtag cggaaaagcg cgggccgcgg
     1381 ccatgctaga gtgagccgca tgactgacgc cggaaccccc aagcctgccc ggcgccctcg
     1441 ctatcgcggc acacacccga gaacgttcga ccagcgttac aaggagaagg accccgcccg
     1501 cttccccacc atggaagcgc acatccgcgc ccagggacgc actcccgccg gatcccacgt
     1561 accggtgatg ctcgacgagt cgatcgacct cctggcgctc gcaccgggca acgtggtcgt
     1621 cgacgccacg ctgggccggg gaggccacgc aagcgccatc ctcgacgcca tcgccccggg
     1681 ggggcgactg atcggtgtgg atcacgacca ggccgagctc gatcgcacgc gcgaacgctt
     1741 ccgtcgcgaa cgccccggcg cccccgtcac ctacgtgcac agcacctttg ccggtcttgg
     1801 caagatccac gccgaacacg cgccggaggg gttcgacgcc gtgctggccg ac
//
LOCUS       DASVOD010000492         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_502087, whole genome shotgun sequence.
ACCESSION   DASVOD010000492 DASVOD010000000
VERSION     DASVOD010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502087"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..1379
                     /gene="pcaF"
                     /locus_tag="VF720_11080"
     CDS             162..1379
                     /gene="pcaF"
                     /locus_tag="VF720_11080"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEX7879945.1"
                     /translation="MKPVYLVDGVRTPIGSFGGSLAHVRADDLAAHAIAKLLERHPNL
                     DRERIADVMLGCANQAGEDNRNVARMALLLAGLPVSVPGETVNRLCASGMSAVANASR
                     AVREGDGDVYIAGGVEQMTRAPYVLSQSSQAFARNAELFDTSLGWRFVNPRMRELYGV
                     DSMGQTAENVATECGISRADQDQFALWSQQKTAASQQRCRFQREIVPVAVAGASRGEP
                     GRLFAQDEFPRPDSTLEALGKLKPAFRTDGKGSVTAGNSSGINDGACALLLASERAVS
                     EFGFTPLARIVATAVAGVEPRTMGLGPVPATRRILAKTKLALPDLDVIELNEAFAAQV
                     LACTRQLGLDDRDPRLNPNGGAIALGHPLGMSGARLLLTAAHELKETGKQHALCTMCV
                     GVGQGMATIIARV"
     gene            1385..>1509
                     /locus_tag="VF720_11085"
     CDS             1385..>1509
                     /locus_tag="VF720_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007096641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX7879946.1"
                     /translation="MPNVFAFEGFIPVVHESAFIHPNATVTGNVVIGRDVYVGPGA"
BASE COUNT          226 a          516 c          517 g          250 t
ORIGIN      
        1 cttgcgtttc cgtgcggcct ccgtgtcttc cgtgcttccg tggtgaatcg cctttcgtgg
       61 tgaatcgcct ttcgcggtga atcgccgttc gtggtgaatc gccgttcgtg gtgaattccc
      121 ctccgtggtt cattcccggt catccgtcta taattctgcc catgaaaccg gtatacctgg
      181 tcgacggagt gcgtaccccg atcggcagtt tcggcgggag tctggcccat gtgcgagccg
      241 acgacctggc ggcgcacgcc attgccaagc tgctcgagcg tcacccgaac ctggatcggg
      301 agcgcatagc cgatgtcatg ctcgggtgcg ccaaccaggc tggcgaggac aatcgcaacg
      361 tggcccgcat ggcgctgctc ctcgccgggc tgccggtgtc ggtgccgggc gagaccgtga
      421 atcggttgtg cgccagcggc atgagcgcgg tagccaacgc gagccgggcg gtgcgcgaag
      481 gcgatggcga cgtctatatc gccggcggcg tcgagcagat gacgcgcgcg ccatacgtgc
      541 tgtcccagtc gagtcaggca ttcgcccgca acgcggagct gttcgacacc agcctgggct
      601 ggcggttcgt gaaccccagg atgcgcgagc tgtacggcgt ggactcgatg ggccagaccg
      661 cggagaacgt agcgacggag tgcgggatca gccgggcgga ccaggaccag ttcgcgctct
      721 ggtcgcaaca gaagaccgct gccagccagc agcgctgccg gttccagcgg gagatcgtcc
      781 cggtcgcggt ggccggtgcc tcccggggcg agcccggccg cctctttgcc caagacgagt
      841 ttccccgtcc ggactcgacc ctggaggcgc tgggcaagct caagcccgcg ttccggaccg
      901 acggcaaagg gagcgtcacc gctggcaact cgtcggggat caacgacggc gcgtgcgccc
      961 tgctgctggc ctccgagcgc gcggtgagcg agttcggctt cacgccgctg gcgcgcatcg
     1021 tcgcgacggc cgtggcaggc gtcgagcccc ggaccatggg actcgggccg gttcccgcca
     1081 cgcgccggat cctggccaag accaagctcg ccctccccga cctggacgtc atcgagctca
     1141 acgaggcctt cgcggctcag gtgctggcct gcacccgcca gctcggcctc gacgaccggg
     1201 atccccggct caatcccaac ggcggcgcca tcgcgctcgg gcacccgctc ggcatgtccg
     1261 gggcccggct gctgctcacc gcggcccacg aactcaagga gaccggcaag cagcacgcac
     1321 tctgcacgat gtgcgtcggc gtggggcaag gcatggcgac gatcatcgcc cgggtgtagg
     1381 gcgcgtgccc aacgtctttg cgttcgaagg cttcatcccc gtggtccacg agtcggcctt
     1441 catccatccc aatgccaccg tgaccggcaa cgtcgtgatc gggcgggacg tgtacgtcgg
     1501 accgggggc
//
LOCUS       DASVOD010000493         1791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1026439, whole genome shotgun sequence.
ACCESSION   DASVOD010000493 DASVOD010000000
VERSION     DASVOD010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026439"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /gene="glgP"
                     /locus_tag="VF720_11090"
     CDS             <1..1173
                     /gene="glgP"
                     /locus_tag="VF720_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX7879947.1"
                     /translation="IDVYHFNEGHAVFAGLELIADRMSRHASFPEAWDWARRRIVFTT
                     HTPVPAGNETHPLAELRRLGAGLELVDNEVEAIGGDPFGMTVAGLRLARLSNAVAQLH
                     GETARKMWKDVSGAAPIIAITNGVHQTTWQAPEIAESRDDSFSLWATHEALKKRLFNR
                     VDELTGVRLKPNALTIGFARRAAGYKRADLIFSDPDRMGALIADGKVQLVFSGKAHPA
                     DGIGKELVARLVHFARKWPASVVFLQDYDMAVARHLVQGADIWLNNPQRPQEASGTSG
                     MKAALNGVLNLSVLDGWWPEGCVHGETGWAIGGTDEGEARDASDAGALYHVLEQDVLP
                     AFGNRQKWTSMMQASIRMAELHFTSHRMVREYYERLYAPESAPVTEPDYQSEGVAG"
     gene            complement(1189..>1791)
                     /locus_tag="VF720_11095"
     CDS             complement(1189..>1791)
                     /locus_tag="VF720_11095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7879948.1"
                     /translation="IERSGRFVEHARRLTSARGLSNVSFQEADLMEAPIEKGPFDAAW
                     CRWVACFVPSPRTLVARVAAVVRSGGTVVFHEYADYRTYGLVPTRPSVTRFVSEVIDN
                     WRSTGGEPDIARALPGLLAAEGFRVERATPRIFAVRPHEELWKWPAGFIETHLDRLIE
                     LGRIDEPFAGQVRSDLADAAQDGVSMLMTPLVLEIVARRS"
BASE COUNT          302 a          603 c          604 g          282 t
ORIGIN      
        1 atcgacgtgt accacttcaa cgaagggcac gctgtcttcg ccggcctcga gttgatcgcc
       61 gaccggatgt ccaggcacgc ctcgttcccc gaggcgtggg actgggcccg tcggcgcatc
      121 gtcttcacga cgcacacgcc ggtgccggcc ggcaacgaaa cacatccgct ggccgagttg
      181 cgccggctcg gcgcggggct ggagctggtc gacaacgaag tcgaggccat cgggggcgac
      241 ccgttcggca tgacggtggc cggcctcagg ctggctcgcc tgtcgaacgc cgtggcccag
      301 ctccacggcg aaacggcccg gaagatgtgg aaggacgtga gcggggccgc gcccatcatc
      361 gccatcacca acggtgtcca ccagaccacc tggcaggcgc ccgagattgc cgaatcgcgc
      421 gacgattcct tttcgctctg ggccacgcac gaagcgctga agaagcggct tttcaaccgg
      481 gtggatgaat tgaccggcgt tcgcttgaag cccaatgcgt tgacgatcgg attcgcccgc
      541 cgcgcggccg gctacaagcg cgccgacctg atcttttccg atccggaccg gatgggcgcc
      601 ctcattgccg acggaaaggt gcagctcgtc ttttcgggca aggcgcaccc cgccgacggc
      661 atcggcaagg aactcgtggc ccggctggtg cacttcgcgc ggaaatggcc ggcctcggtg
      721 gtcttccttc aggattacga catggcggtg gcgcgtcatc tcgtgcaggg ggccgacatc
      781 tggctcaaca acccgcagcg cccgcaggag gccagcggca cctcgggaat gaaggcggcg
      841 ctcaacggcg tgctgaacct gagcgttctc gacggctggt ggcccgaggg gtgcgtgcac
      901 ggcgagaccg gctgggccat cggaggcacc gacgaaggtg aggcgcgcga tgcttcggac
      961 gcgggcgccc tctatcacgt gctcgagcag gacgtccttc cggcgttcgg caaccggcag
     1021 aagtggacct cgatgatgca ggcgtccatc cggatggccg aattgcattt caccagccat
     1081 cgcatggtgc gcgagtacta cgaacgcctc tacgcgccgg agtcggcccc ggtcaccgag
     1141 cccgactacc agtccgaagg cgtagccggc tgattcccgc ctccgcggtc acgaccggcg
     1201 ggcgacgatc tcgaggacca gcggcgtcat cagcatcgat acgccatcct gcgcggcatc
     1261 cgccaggtcg ctccgaacct gtcctgcaaa gggctcgtca atccgaccga gctcgatgag
     1321 ccggtccagg tgcgtttcga tgaatcccgc cggccacttc cacaattcct cgtgcggcct
     1381 caccgcaaag atgcgcggcg tggcgcgctc cacccggaat ccttcggccg cgagcaggcc
     1441 gggaagtgcg cgcgcgatgt ccggttcacc gccggtcgag cgccagttgt cgatgacttc
     1501 gctgacgaag cgcgtgacgc tcggccttgt cggaacgagg ccataggtgc ggtagtcggc
     1561 gtactcgtgg aagacgaccg ttccgccgga ccggacaacg gccgccactc tggccaccag
     1621 ggtgcgcggc gaggggacga agcaggctac ccagcggcac cacgcggcgt cgaagggccc
     1681 tttctcgatc ggcgcctcca tcaggtcggc ttcctggaag cttacgttgg agaggccgcg
     1741 ggcgctcgtc agtcgccggg catgctcgac gaagcgaccg gagcgctcga t
//
LOCUS       DASVOD010000494         4644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_815063, whole genome shotgun sequence.
ACCESSION   DASVOD010000494 DASVOD010000000
VERSION     DASVOD010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815063"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1017)
                     /locus_tag="VF720_11100"
     CDS             complement(<1..1017)
                     /locus_tag="VF720_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879949.1"
                     /translation="MTFPGVLADPTRAEPGSLLAIALLVVGLSMPAIAHGSDPATPCA
                     IIVPSGFAVAPADTCEWSGRCLADIDFGPRGTSDGKTILWGAGARGPSMGWTGGRVFD
                     FIDRRRRETPLRDGIEGIDSRFSIGVSEPDSLYRLTIWLADTLRVTAPLSVDVGHAHV
                     ADEVLPVAGRCRSMRWLVRPASDRVSFRLVAPVCKRFAITAARLEGPYDARVTVIFPD
                     VDTTRAAFPPAGELPFMTEALLRDQIRRDAEHLIAERLSDGRFSQHGAWYENSFPIRA
                     LVSAGELLNEPAWSETAFQLLDDFVAHQRPDGNWASDYNDPEECPGGVAPPVSANLAD
                     IGTMT"
     gene            1258..2187
                     /locus_tag="VF720_11105"
     CDS             1258..2187
                     /locus_tag="VF720_11105"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX7879950.1"
                     /translation="MDLVVAALTGLSRTWIRGLFDHGGVTMNGHPAPDPGGRVAAGDF
                     VEVTWDPHNRTREKRRPWIDPAFRVLYEDEHVIVVDKAAFVLTVPTQHGETDTLVQRV
                     RRYLGKGGAEGKATVVQRLDHGTSGVMVFAITEAAASGLIEQFKRHTPAREYVALVAG
                     LVKDDRGTIESRLATNRRLSRYSTTSGEGERAVTHFEVEARLQDATRIRVRLETGRRN
                     QIRVHFAEAGHPVLGDTRYRPELARHPRWRSRRQALHAAVLGFTHPITGQSMRFDSPL
                     PAEFGTFREVAAPLTPRRRRSRRSGPPGTSTPA"
     gene            complement(2126..3103)
                     /locus_tag="VF720_11110"
     CDS             complement(2126..3103)
                     /locus_tag="VF720_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7879951.1"
                     /translation="MRYRHVGKAGIQVGELSFGSWVTFGTQVDVDAAAAMMAAAYDSG
                     VNFFDNAQVYAGGKAESVMGAALARLGWRRGSYLVSTKFYWGLHDNPNEKNTLNRKFL
                     LEGIDGSLQRLGLDHVDIIYCHRADASTPIEETVRAMHSIIERGKALYWGTSEWTAAE
                     IVQAIEIAERHHLHKPMVEQPVYNLFEQHRFSPEYIRVYEDYGYGSTTWSPLASGLLT
                     GKYKEGIPKESRGALPGYTWLAEQLTDADKAARVAALEPMAREMGATLAQFSLAWCLR
                     NPHVSSVILGASRIEQLRENLKAVDFVDRFTPELMSRVDRIFGIDGAVG"
     gene            complement(3100..>4644)
                     /locus_tag="VF720_11115"
     CDS             complement(3100..>4644)
                     /locus_tag="VF720_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7879952.1"
                     /translation="FKGESSAVVFEAILNRTPPRVSHASPMLPADLDRVLEKALEKDR
                     SLRYQSATEIKTDLLRLKRDTDSGSRRATDVAESRSGLAAQAAEKSIAVLYFENLSGI
                     KDDEYLRDGVTEDIITELSKIKGLRILSRPTVLPYRDKPVTAAQVGQQLKAAVVLAGS
                     IRRAGNRLRITAQLVDAQTDSLVWSERYDREMADVFEVQDEIARKIAEALRITLSPQE
                     EKALAARPTDNLQAYDLYLRGKSYARRMTHQDTEFALQMFESAVSMDPGFALAHAAIA
                     NVCAETYYRSARTAVWIERAKAASQRASSIGGDSAEIRMAEAWILYAEGKYDEAVPRT
                     RQAIAMKSDVEGGYHLLGRVLFAAGRYQEVADMAEEAVAAAPEDYNVYIPLGNAFEAL
                     GKKDAARNMALQNVQVLEAHLQKFPEDARARVLLATYYAELGRPDDAMRELGLAMAMR
                     SNDASVLYNAACAYCFLQKKPEAINALKKAWERGWRDGGWARRDSDLSILHGDPDFEK
                     LYPEGA"
BASE COUNT          748 a         1527 c         1586 g          783 t
ORIGIN      
        1 cgtcatcgtg ccgatgtcgg ccaggttggc gctgacgggg ggcgcgactc cacccgggca
       61 ctcctcggga tcgttgtaat ccgatgccca gttgccgtcg gggcgctggt gcgccacgaa
      121 gtcgtccagg agctggaaag ccgtttcact ccaggcgggt tcgttcagga gctcgcccgc
      181 cgacaccagt gcccggatgg ggaatgagtt ctcgtaccag gcgccatgct ggctgaaccg
      241 cccgtcgctg agcctttccg cgatcaggtg ctcggcgtca cggcggatct ggtcgcgcag
      301 cagtgcctcg gtcatgaagg gaagctctcc ggccgggggg aacgccgcac gcgtcgtatc
      361 gacatccggg aaaatcaccg tgacccgcgc gtcgtacggg ccttccagcc gggcggccgt
      421 gatcgcgaag cgcttgcaga ccggagccac caggcggaac gacacccggt cggacgccgg
      481 ccggacgagc catcgcatcg agcggcatcg tcccgccacg ggaaggacct cgtccgccac
      541 gtgggcatgc ccgacatcca ccgacagggg agcggtcacg cgcagcgtgt cggccagcca
      601 gatcgtgagg cgatagaggg agtcgggttc gctcacgccg atcgagaagc gcgagtcgat
      661 tccttcaatg ccatcgcgca agggagtttc gcgccgccgt cgatcgatga agtcgaagac
      721 ccgcccgccg gtccatccca tgctggggcc gcgcgctccg gcaccccaca ggatggtctt
      781 tccatcgctc gtaccgcgcg gcccgaagtc gatgtcggcc aggcagcggc cgctccactc
      841 acacgtgtcc gcgggagcga cggcaaaacc ggacgggacg atgatggcgc aaggagtggc
      901 ggggtcggag ccatgggcga tcgcgggcat ggaaaggcca acgacgagga gggcaatcgc
      961 gaggagcgat cccggttccg cccgggtggg gtcggccagt accccgggga aagtcatcag
     1021 gagcgcgacc gcatgcaggc agtcgtgcgc atgtaaatat tataggggtg acgctcggaa
     1081 aagctgacgg aattcagcga tccatggcac agtctgatat gcgttagaac actaatcggg
     1141 ccgagtggcc atgacagtga tgccggcacc gacaccggaa cgccggattt cgcgaaggga
     1201 tttcaactgg cgaagctgac gaagaaagcc accatcctgc cggagcaggc gggccggatc
     1261 gacctggtcg tggcggccct gaccggcctc tcgcggacct ggatccgtgg attgttcgat
     1321 cacggcggcg tgacgatgaa cggccatccc gcccccgatc ccggcgggcg cgtggcggcc
     1381 ggtgatttcg tcgaggtcac ctgggatccc cacaatcgca cccgggagaa gcggcgcccc
     1441 tggatcgacc cggcgttccg cgtgctgtac gaggacgaac acgtgatcgt ggtggacaag
     1501 gcggcgttcg tgctgacggt gccgacccag cacggggaga ccgacaccct ggtccagcgc
     1561 gtgcgacgct acctggggaa ggggggggcg gaggggaagg ccaccgtggt gcaacgtctc
     1621 gatcacggca cctcgggtgt catggtattc gcgatcaccg aggccgcggc gtcagggttg
     1681 atcgaacaat tcaagcgcca cacgccggcg cgcgaatacg tggcgctcgt cgccgggctg
     1741 gtcaaggatg accgtggcac gatcgagagc cgcctggcca cgaaccggcg cctttcgcgc
     1801 tactccacca ccagcgggga aggggaacgg gccgtcacgc atttcgaagt ggaggcgcgg
     1861 ctccaggatg ccacgcgcat ccgcgtacgg ctggagacgg ggcgacggaa ccagatccgc
     1921 gtgcactttg ccgaggcggg ccatccggtt ctgggtgata cgcgctatcg tccggagctg
     1981 gcccggcatc cccgctggcg ttcgcggcgg caggcactgc atgccgccgt gctgggattc
     2041 acgcacccga tcacgggcca gtcgatgcgc ttcgactcgc cgctgccggc ggaattcggg
     2101 acatttcggg aggtggcggc cccgctcacc ccacggcgcc gtcgatcccg aagatccggt
     2161 ccacccggga catcaactcc ggcgtgaacc gatcgacgaa gtccacggcc ttcaggttct
     2221 cacggagttg ttcgatgcgg ctcgcgccga ggatcacgct cgagacatgc gggttccgga
     2281 ggcaccaggc gagcgaaaac tgggccagcg tggcgcccat ctcgcgggcc atcggctcga
     2341 gtgcggccac ccgggccgcc ttgtccgcgt cggtgagctg ctccgccagc caggtgtagc
     2401 ctggcaaggc gccgcggctc tccttcggga tgccctcctt gtacttgccg gtcaggagcc
     2461 ccgaagccag cgggctccag gtggtgctgc cgtagccgta atcctcgtac acgcggatgt
     2521 actcgggcga aaagcgatgc tgctcgaaga ggttgtacac cggctgctcc accatcggct
     2581 tgtggaggtg atgccgctcg gcgatctcga tggcctgcac gatttccgcg gcggtccatt
     2641 ccgacgtacc ccagtaaagc gcctttcccc gctcgatgat gctgtgcatc gcgcgcacgg
     2701 tctcctcgat cggcgtgctc gcgtcggcgc gatggcaata gatgatgtcc acgtggtcca
     2761 ggccgaggcg ctggagcgag ccatcaattc cctcgagcag gaacttccgg ttgagcgtgt
     2821 tcttctcgtt ggggttgtcg tgcaatcccc agtagaactt ggtggagact agataactcc
     2881 cgcgccgcca tccgagcctg gcgagcgccg cgcccatgac cgactccgcc ttgccgccgg
     2941 cgtacacctg ggcgttgtcg aagaaattga ccccggaatc gtaggccgcc gccatcatgg
     3001 ccgcggcggc atcgacatca acctgcgtgc cgaaggtgac ccaggaaccg aacgagagct
     3061 cgcccacctg gatgccggcc ttcccgacgt ggcggtatct catgcaccct ccgggtagag
     3121 cttttcgaag tcgggatcgc cgtggaggat cgacaggtcg gaatcgcggc gggcccagcc
     3181 gccgtcacgc cagccgcgct cccaggcttt cttcagggcg ttgatggcct cgggcttctt
     3241 ctgcaggaag cagtaggcgc aggccgcgtt gtagagcacc gacgcgtcgt tcgaacgcat
     3301 ggccatggcg aggcccagct cccgcatcgc gtcgtccggg cggcccagct cggcatagta
     3361 ggtggccagc agtacccggg cccttgcatc ctcggggaat ttctggagat gggcctccag
     3421 cacctgcacg ttctgcagcg ccatgttccg cgccgcgtcc ttcttcccga gggcctcgaa
     3481 ggcattcccg agcgggatgt agacgttgta gtcctcgggc gccgccgcca cggcctcttc
     3541 cgccatgtcg gccacttcct ggtaccggcc ggcggcgaag agcacgcgcc ccagcaggtg
     3601 atagccccct tccacgtcgg acttcatggc gatggcctgc cgcgtgcggg ggaccgcctc
     3661 gtcgtacttc ccctcggcgt acaggatcca cgcctcggcc atgcggattt ccgccgaatc
     3721 gccgccgatg gaactggccc gctggctggc cgccttggcg cgctcgatcc agacggccgt
     3781 gcgcgccgag cggtagtacg tttcggcgca gacgttggcg atggccgcgt gcgcgagcgc
     3841 gaaacccggg tccatgctca ccgcgctctc gaacatctgc agcgcgaatt cggtgtcctg
     3901 gtgcgtcatg cgtcgcgcgt aactcttgcc gcgcaggtag aggtcgtagg cctgcaggtt
     3961 gtcggtggga cgggccgcca gcgccttttc ttcctgcggc gagagggtga tgcgcagcgc
     4021 ctcggcgatc ttccgggcga tctcgtcctg tacctcgaac acgtcggcca tctcccggtc
     4081 gtagcgctcg gaccagacga gcgagtcggt ctgcgcgtcc accagttgag ccgtgatccg
     4141 gagccggttg ccggcccgcc ggatgcttcc ggccagcacc acggcggcct tgagctgctg
     4201 gcccacctgg gccgccgtga ccggcttgtc gcggtagggg agcaccgtgg gtcgcgagag
     4261 gatccgaagt cccttgatct tcgagagttc ggtgatgatg tcctcggtga cgccgtcacg
     4321 caggtattcg tcatccttga ttccggaaag gttctcgaag tagaggacgg cgatcgactt
     4381 ttccgccgcc tgcgccgcga ggccggagcg cgactccgcc acgtccgtgg cccgccggct
     4441 tcccgagtcg gtgtcccgct tcaggcgcag gaggtcggtc ttgatctcgg tggccgactg
     4501 gtaccggagc gaacggtcct tttccagggc cttttccagc acgcgatcca ggtcggccgg
     4561 gagcatcggg ctggcgtggc tcacgcgggg cggagtccgg ttgaggatgg cttcgaacac
     4621 gacggccgac gattctccct tgaa
//
LOCUS       DASVOD010000495         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_189366, whole genome shotgun sequence.
ACCESSION   DASVOD010000495 DASVOD010000000
VERSION     DASVOD010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189366"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF720_11120"
     CDS             <1..241
                     /locus_tag="VF720_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HEX7879953.1"
                     /translation="PVVAGDEIRVSLERQLLGAVRWEESMRWLTANAGMHMVEIGPGR
                     VLKGLMRSIDRAASVANVEDPEGVAAFMAPEGDSK"
     gene            238..996
                     /gene="fabG"
                     /locus_tag="VF720_11125"
     CDS             238..996
                     /gene="fabG"
                     /locus_tag="VF720_11125"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016201081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX7879954.1"
                     /translation="MTDSTLPLAGRTAFVTGGSRSIGEAIVMELARRGANVVVTDSGG
                     GGAERVAAAVEALGRRSLALSFDVSDSARVDEAIKAAEALFPAVDILVNNAGITRDQL
                     LIRMKDEDWDRVQAVNLKGAFNCTRAAVRGMMKRRWGRIVNITSVVGIIGNPGQANYA
                     AAKAGLIGMTRAVARELAGRNVLVNAVAPGFIDTAMTEVLTDQVKQAMKDSIPLGRFG
                     RAEEVARVVAFLASGDADYVTGQVFNVDGGMVMA"
     gene            1073..1309
                     /gene="acpP"
                     /locus_tag="VF720_11130"
     CDS             1073..1309
                     /gene="acpP"
                     /locus_tag="VF720_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX7879955.1"
                     /translation="MAFSEEKVREIIVEQLGVSADQVKPEARFIDDLGADSLDTVELI
                     MALEEEFGVEIPDEDAEKMVTVGDALNYLKEHAS"
     gene            1306..>2392
                     /gene="fabF"
                     /locus_tag="VF720_11135"
     CDS             1306..>2392
                     /gene="fabF"
                     /locus_tag="VF720_11135"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX7879956.1"
                     /translation="MSGPTPTHRRVAVTGLGVVSPVGNDVESFWNSLIGGKSGITRVT
                     RFDAGDFRTQIAAEVKGFDASAYMDKKEVRRTDLYVQYAIAASAQALKDSGLDLAAED
                     LTRIGVIIGTGIGGIATFEEQTEVMLAKGPSRVSPFFIPMMIANMASGQVSIQFGLKG
                     PNTTTVSACASGAHAIGEAFQAIRHGQADVMVTGGSEATITKMAMAGFCALKAMSTRN
                     DDPERASRPFERDRDGFVMGEGAGVLVLEDYDHAKARGATILAEMVGYGTTGDAYHMT
                     APPPEGEGAARAMAMALADGGLQPADVDYINAHGTSTDLNDKYETAAVKTVFGTDAAR
                     LAMSSTKSMTGHLLGARRHRSHGGHGGL"
BASE COUNT          419 a          778 c          809 g          386 t
ORIGIN      
        1 ccccgtggtt gcgggcgacg aaatccgcgt ctccctcgag cggcaactcc tcggcgccgt
       61 ccgctgggag gagtcgatgc gctggctcac cgccaacgcc ggcatgcaca tggttgaaat
      121 cggccccggt cgcgtgctca agggactgat gcgctcgatc gaccgggccg catccgtggc
      181 gaatgtcgaa gacccggagg gggtggccgc cttcatggcg ccggagggag actcgaaatg
      241 accgattcca cgttgccgct ggccggccgg acggccttcg tgaccggcgg atcccgcagt
      301 atcggcgaag ccatcgtcat ggaactggcc cgccgggggg caaacgtcgt cgtgaccgac
      361 tccggcgggg gcggggctga gcgggtcgcg gccgccgtcg aggccctggg gcgccgctcc
      421 ctggcgctct ccttcgatgt gtccgattcg gcccgggtcg acgaggccat caaggcggcc
      481 gaggccctct ttcccgccgt cgacattctg gtcaataatg ccggcattac ccgggatcag
      541 ctccttatcc ggatgaaaga cgaggactgg gaccgggtgc aggcggtcaa cctgaagggg
      601 gccttcaatt gcacccgggc cgcggtccgg ggcatgatga agcgccgctg ggggaggatc
      661 gtcaacatca cctcggtcgt cggcatcatc ggcaaccccg gccaggcgaa ctacgcggcc
      721 gccaaggccg gcctgatcgg catgacccgg gccgtagccc gggaactggc cggcaggaat
      781 gtcctggtca acgcggtggc ccccgggttc atcgatacgg ccatgacgga agtcctgacc
      841 gaccaggtca agcaggccat gaaggattcg attcctctcg gtcgtttcgg ccgggcagag
      901 gaagtggcaa gagtggtggc gttcctcgcg tcgggggatg ccgattacgt caccggacaa
      961 gtgttcaacg tcgacggcgg catggtcatg gcctgaccgc cccccggcat tgtcgccggg
     1021 ggccgggctg tgccggcacc gtcgttatta cccgtgtgag gaggttttcg gaatggcgtt
     1081 cagcgaggaa aaggttcggg agatcatcgt cgagcagctc ggcgtttcgg ccgaccaggt
     1141 caagcccgaa gcgcggttca tcgacgatct cggtgccgat tcgctcgaca ccgtcgagct
     1201 catcatggcg ctcgaggagg agttcggcgt ggaaattcct gacgaggatg ccgagaagat
     1261 ggtcaccgtc ggcgatgccc tcaattacct gaaggagcac gccagctgag cgggcccacc
     1321 cccacccatc gtcgcgtcgc cgttacgggc ctcggcgtcg tctccccggt cggaaacgac
     1381 gtcgagtcct tctggaactc gttgatcggc ggaaagagcg gcatcacccg tgtcacccgt
     1441 ttcgacgcgg gcgatttccg cacgcagatc gccgccgagg tcaaggggtt cgacgcctcg
     1501 gcttatatgg acaagaagga agtccggcgg acggatctct atgtccagta cgccatcgcc
     1561 gcgtcggcac aggccttgaa ggattcgggc ctcgatctcg ctgccgagga cctgacgcgc
     1621 atcggcgtca tcatcggcac cgggatcggt gggatcgcca ccttcgagga gcagaccgag
     1681 gtcatgctcg cgaaggggcc atcccgcgtc agcccgttct tcattcccat gatgatcgcc
     1741 aacatggctt ccggacaggt gtcgatccag ttcggcctga agggtcccaa caccaccacc
     1801 gtctcggcct gtgcctccgg ggcgcatgcc atcggcgaag cgttccaggc catccgtcac
     1861 ggacaggccg acgtcatggt caccggcggc agtgaggcca ccatcaccaa gatggcgatg
     1921 gccggtttct gtgccctgaa ggccatgtca acccggaacg acgatccgga acgcgcgtcg
     1981 cgacccttcg agcgtgaccg ggatggattc gtgatgggag agggcgcggg cgtcctggtg
     2041 ctcgaggact acgaccacgc gaaggcccgc ggcgccacga tcctcgccga aatggtgggg
     2101 tacggcacga cgggcgacgc ctatcacatg accgcgcccc ccccggaagg tgagggggcc
     2161 gcacgcgcca tggcgatggc cctggccgac ggcgggctcc agccggccga cgtcgactac
     2221 atcaatgccc acggcacatc gaccgatctc aacgacaagt acgaaactgc cgccgtgaag
     2281 accgtgttcg gcaccgacgc ggcgcgactc gccatgtcgt ccaccaagtc gatgaccgga
     2341 cacctgctcg gcgcgcgaag gcatcggagc cacggaggcc atggcggcct ga
//
LOCUS       DASVOD010000496         8963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_241266, whole genome shotgun sequence.
ACCESSION   DASVOD010000496 DASVOD010000000
VERSION     DASVOD010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241266"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF720_11140"
     CDS             <1..413
                     /locus_tag="VF720_11140"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX7879957.1"
                     /translation="MAAGRILRTGAGTVGLMGLTFKAGTDDLRESPQVELAASLIARG
                     RSLLIYDPNVQLSHIVGLNREYLQSRIPRISEILCASPERLIAECGVLVVGNKTAEYK
                     AALQSTRPEQTVVDLVRLVDDPAGLNAAYDGLCW"
     gene            427..2778
                     /locus_tag="VF720_11145"
     CDS             427..2778
                     /locus_tag="VF720_11145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7879958.1"
                     /translation="MNGHKVLILVENYPLPFDRRVWLEAKTLRAAGYQVSIICPKGKG
                     ATESFVEIDGIRIYRYTAWEAKKGALAFAFEFSRCWLRTFLLTIKVLFKDGFDVIHAC
                     NPPDTFWVIGAIYKILGKRFIFDQHDLNPEVYLSKFPDQDGDFAHRMLYWFEKRTYRT
                     ADVVIAMNESYRNTAISRGGVDPDRVIVVRTGPDFERLHPVPAEPGLKQGADFLVAYL
                     GTMAPQDGVDYLLQAAAHIVHERGRTNVRFVFIGSGSSLDDLKALAGTLNLADRCTFT
                     GRVSDADLRRWLATADVCASPDPRNPLNEVSTMNKTMEYMAMGKPVVSFALKETIESA
                     GEASLYAEPNRTDEFGDRIIELLDDPARRESMGEFGRTRVHEKLAWNHSEPHLLKAYA
                     KALDGVPARRLRILMIGQKGVPATYGGIERHVEEIGARMVQRGHEVSVYCRPHYTRQR
                     GKHRGMGMIVRPSLNTKHLDTLTHTLVCSIDALFRRVDVVHYHALGPSPFALLPRLRG
                     IKTIVTVHGLDWEREKWGMLAAYILQRCEYPAIHFPNATIVVSQTLENYFAEKYRIRP
                     RHIPNGTPEPVRRAPAEIRQYGIGEKDYILFVGRLTPEKGAHFLMEAFGRIDTPMKLV
                     IAGGTSFTDDYVASLEAMKTDRVILTGYVYGDTLAELYTNAHLVVLPSTMEGLSIALL
                     EAVSYGKCVLISDIPENVEVMNECAPRFRSRDVDHLETQLRHLLDNPAEVAEFEAMTR
                     DRISRRFSWETIVDAIETTYLEVDARRYVARAEAPIESPVPAT"
     gene            2775..3863
                     /locus_tag="VF720_11150"
     CDS             2775..3863
                     /locus_tag="VF720_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEX7879959.1"
                     /translation="MTAPPNVAVILAGGRGERFWPLSSAGRAKQFLSLAGSDTLLTRT
                     ARRLHPLVTVDNLLVVTGHDQVGLVHESLPEILPSQIVAEPMGRNTAPAVALGALWAE
                     RRHPGAMLVVVPSDAWVGDDAAYRAALSAGLDRTRTHPALATIGIVPSRPETGYGYLE
                     VGSAIDGAASLFQVNRFVEKPDAATAAAWLAGGRHLWNGGIFLMPAATLLEAVRRHLP
                     DVAGPLDGLDAATPKGATGFDAAALLAYYGAVPSISIDYGVMEKSDNVITVRGAFPWD
                     DLGAWSALERVLPNVDGVTAVGATLAIDSPGAILYADEGLVAALGLPDVIVVRAGKSV
                     LVVPKSRAQDVRRLVQAIEARDDLKGFR"
     gene            3860..4291
                     /locus_tag="VF720_11155"
     CDS             3860..4291
                     /locus_tag="VF720_11155"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEX7879960.1"
                     /translation="MRLGRGIAIAPLALVVLAAGCGGGRKAQPTAEPVAVTPVGEQVP
                     GPVLSPRAVVPLVSADQLVDGYRVQLFQSTQLRDCEQFRDAAAVRLDKPVYVEYLSPL
                     YRVRAGDFLTREAADAWRDGLAAQGFERAETVSSRVRSGGG"
     gene            4399..5409
                     /gene="galT"
                     /locus_tag="VF720_11160"
     CDS             4399..5409
                     /gene="galT"
                     /locus_tag="VF720_11160"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEX7879961.1"
                     /translation="MPELRKDPVVGRWVIISTDRGRRPSDFHIQTTPRDSKNCPFCPG
                     HESQTPPEVLAVRHNSSGPNAPGWKVRVVPNKFPALQIEGDLERRGEGLFDKMNGIGA
                     HEVIIESTDHHAELSTLPVDDVAQVLGAYRDRVVDLKGDPRFKYILVFKNWGEAAGAT
                     LQHPHTQLIAMPIIPKWVSEELKGSRSHFEMKERCIYCDILQQETSDGRRMVTADEHH
                     VAIVPFAPRFPFETWILPRKHSSSFESMNDDALWNLARMLKETLTRMNAALNHPPYNF
                     IIHTAPCNDPWLLYYHWHIEIMPKLTKVAGFEWGSGFYINPTSPEDAARDLRNAVPVE
                     TA"
     gene            5409..6974
                     /locus_tag="VF720_11165"
     CDS             5409..6974
                     /locus_tag="VF720_11165"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HEX7879962.1"
                     /translation="MARSTLDLPGQTSQASSGGKTPLEIAVVTTEFAPLARVGGLADV
                     AASLSAEMARLGASITVWMPAWRQVWESGAALRPVAVPRPLVVPLGAESVPVDLLEVV
                     TPPPGVRIFLIDYPPFFGRSGIYVDPATGEPWLDHDRRLVLFQRAVLEAFQRLDLRPD
                     VIHCHDHQTGLIPVYLKVKDGLDPFFAGTGTLMTIHNLGYQGLFPAEVLEMARLPMDL
                     FYPSGPLEFWGRVNFMKGGILHADRLTTVSPRYAEEIQASEEIGFGLEGVLRTRAADL
                     VGILNGIDAAMWNPATDPVLAANFDANDLSGKAACRDDLVTRLGLTGVTAATPLVAML
                     SRLVDAKGFDLLVSQFPAMIEAGWRPVIIGSGPRKNEEAMARLRDRFPGQLHWARSAD
                     DALAHRIEAGADFYLMPSKYEPCGLNQMISMRYGALPIVRETGGLIDTVASYDPATGA
                     GTGFGFRDYTPEGLQAALRLARQVFDDPARLTAVRRQAMSMDFSWSRPARAYLALAEE
                     IAAKRTPLRRRNS"
     gene            7185..>8963
                     /locus_tag="VF720_11170"
     CDS             7185..>8963
                     /locus_tag="VF720_11170"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7879963.1"
                     /translation="MRHRNPLTRTITVATGMASSAYCLLVGQLVDVNQPAAGVDLARL
                     LLFLAAPACLAGHLFALTVGRADPAGSIRRHRLSLVVQALVSMAFLAFLPGPGFVQRF
                     AFSNGDYGLELGAIGRFYLVFLLLGTALIGFRLENTLRGSDPTHRERMRWLALGVFGM
                     VGYFVYLLTDGAVNGRLDQDVLGASLIPMGLAAVLAGWTQLRGGLADAGVQVGRQVIF
                     RSLTFLFVVGYVVVVLLLGELAEMLGIRPSFIAGATIVIGTATGLGAFLLSTPFRRTM
                     RRIVEKNVYSNRLDYRTWWLRASHELVSGIGVSELILRTQTMLEDLFHSRTIVIYLSR
                     RPGEAYQPMNAVMAVPPPDVEAGELLVAALEGAAEPLLLPERRDRSVEPELLPILVEN
                     EAFLKATDGRILAPLRTTERLIGFVVLGPRADGSDYGYEDRSLLETVTLQLAGAIRGA
                     WLTEDLGAAREMEVFSQWAGVVVHDLKNFVSPLKLFVQNARRHLHNAEFREQAIDDIQ
                     GVVEKIDGAIQRLTALKHGEEIALAPTDLEWILDSVLTRSGVSMRPGYSVARNFGGVP
                     SVVADQLLIDRVLTNLVTNAMEAMPEG"
BASE COUNT         1407 a         3160 c         2917 g         1479 t
ORIGIN      
        1 agatggcggc cggccggatc ctgcgcaccg gcgccggcac cgtggggctc atgggcctca
       61 ccttcaaggc cggcaccgac gacctgcgcg aatcgccgca ggtggagctg gccgcctcgc
      121 tcatcgcccg cgggcggagc ctcctcatct acgacccgaa cgtgcagctc agccacatcg
      181 tggggctgaa ccgcgagtat ctccagagcc gcatcccccg catttcggaa atcctctgcg
      241 cctctcccga acggctcatc gccgaatgcg gcgtgctggt ggtggggaac aagaccgccg
      301 agtacaaggc agcgctccag tccacccgcc ccgaacagac cgtggttgac ctggtgcgcc
      361 tggtcgatga tccggccggc ctcaacgcgg cttatgacgg gctctgctgg tagcgggatt
      421 tccccggtga acggccacaa ggtcctcatc ctcgtcgaga actacccgct gccgttcgac
      481 cggcgcgtgt ggctcgaggc aaaaacgctc agggccgccg gctaccaggt atcgatcatc
      541 tgccccaagg gaaagggggc caccgagtcg ttcgtcgaga tcgacggcat ccggatctac
      601 cgctacaccg cctgggaggc caagaagggc gcgctggctt tcgccttcga gttctccagg
      661 tgctggctcc ggacgttcct cctcaccatc aaggtgctgt tcaaggacgg cttcgacgtc
      721 atccacgcct gcaacccgcc cgacacgttc tgggtgatcg gcgccatcta caagatcctc
      781 ggcaagcgct tcatcttcga ccagcatgac ctgaatcccg aggtgtacct ctccaagttt
      841 cccgaccagg acggcgactt cgcccatcgg atgctctact ggttcgagaa gcgcacctac
      901 cggacggccg acgtcgtcat cgccatgaac gagtcgtacc ggaacaccgc catctcgaga
      961 ggcggggtcg accccgaccg ggtgatcgtg gtgcggaccg gacccgactt cgagcggctc
     1021 caccccgtgc ccgccgagcc cgggctcaag cagggggccg acttcctggt ggcttacctg
     1081 ggaacgatgg ccccgcagga cggcgtcgac tacctgctgc aggccgccgc gcatatcgtg
     1141 cacgagcgcg gccggacgaa cgtgcgtttc gtcttcatcg gttcgggctc ctcgctcgac
     1201 gacctgaagg cgctggccgg cacgctgaac ctggccgacc gctgcacctt caccggccgc
     1261 gtgtcggatg ccgacctgcg ccgctggctc gccacggcgg acgtctgcgc cagccccgat
     1321 ccacgcaacc cgctgaacga agtgtcgacg atgaacaaga cgatggaata catggcgatg
     1381 gggaagccgg tcgtgtcgtt cgcgctcaag gaaacgatcg aatcggccgg cgaggccagc
     1441 ctgtatgccg agcccaaccg gaccgacgaa ttcggcgacc ggatcatcga actcctcgac
     1501 gatccggccc gccgcgagtc gatgggggaa ttcgggcgga cgcgcgtgca cgagaagctg
     1561 gcctggaacc attcggagcc gcatcttctc aaggcctacg cgaaggcgct cgacggcgtg
     1621 ccggcgcggc gcctgcgcat cctgatgatc ggccagaagg gtgttccggc cacttacggc
     1681 ggcatcgaac gccacgtgga ggagatcggg gcccgcatgg tgcagcgcgg ccacgaggta
     1741 agcgtgtact gccggccgca ctacacccgc cagcgcggca agcatcgcgg catggggatg
     1801 atcgtccgcc ccagcctcaa caccaagcat ctcgatacgc tcacgcacac gctggtctgc
     1861 tcgatcgacg ccctcttccg ccgggtggac gtggtgcatt accacgccct ggggccgtct
     1921 cctttcgccc tgctgccgcg cctgcgcggc atcaagacca tcgtgacggt gcacggcctc
     1981 gactgggagc gggagaagtg ggggatgctg gccgcctaca tcctgcagcg gtgcgagtac
     2041 ccggccatcc acttcccgaa cgccacgatc gtggtgtcgc agacgctcga gaactacttt
     2101 gccgagaagt accggatccg cccccgccac atcccgaacg gcacgccgga gccggtgcgg
     2161 cgcgcgccgg ccgaaatccg acagtacggg atcggcgaaa aggattacat tctcttcgtg
     2221 ggccgcctca cacccgagaa gggtgcgcat ttcctcatgg aggccttcgg gcgcatcgac
     2281 acgccgatga aactggtgat cgccggcggc acctcgttca ccgacgacta cgtggcctcg
     2341 ctcgaggcca tgaagaccga ccgggtcatc ctgaccggct acgtctacgg cgacacgctg
     2401 gcggagctct acacgaacgc gcacctggtg gtgctcccct ccaccatgga agggctctcc
     2461 atcgcgctgc tcgaggcggt gtcgtacggg aagtgtgtcc tgatcagcga cattcccgag
     2521 aacgtggaag tgatgaacga gtgcgcgccc cgattccgga gccgtgatgt ggaccatctc
     2581 gaaacccagc tccggcacct gctcgacaac ccggccgagg tggcggaatt cgaggccatg
     2641 acccgcgacc ggatcagccg ccgtttctcg tgggagacga tcgtcgacgc catcgagacg
     2701 acctacctcg aggtggatgc ccggcgctac gtggcccggg ccgaggcccc gatcgaatcg
     2761 ccggtgcccg ccacgtgacc gcgccgccga acgtcgcggt catcctggcc ggggggcgcg
     2821 gcgagcgctt ctggccactc tcctcggccg gacgcgccaa gcagttcctt tcgctggccg
     2881 ggagcgacac gctcctcacg cgcacggcgc gccgtctcca tccgctggtg acggtcgaca
     2941 acctcctggt ggtcaccggc cacgaccagg tgggcctcgt gcacgaatcg ctccccgaga
     3001 tccttccgtc gcagatcgtg gccgagccga tggggcggaa taccgctccg gccgtggcgc
     3061 tgggtgcgct ctgggccgag cggcggcatc ccggcgccat gctggtggtg gtgccttccg
     3121 acgcctgggt gggggatgac gcggcctatc gcgcggcact ctcggccggc ctcgaccgca
     3181 cgcgcacgca cccggcgctg gccacgatcg gcatcgttcc ctcgcgtccc gagacggggt
     3241 atggctacct ggaagtcggg agcgcgatcg acggcgcggc cagcctcttc caggtgaacc
     3301 gcttcgtgga gaaaccggat gccgcgaccg ccgccgcgtg gctggccggc gggcgccatc
     3361 tctggaacgg cggcatcttc ctgatgccgg cggccacgct cctggaagcg gtacgccggc
     3421 atcttcccga cgtggcgggt ccgctcgacg ggctcgacgc ggccacgccc aagggcgcca
     3481 ccggtttcga tgcggccgcg ctcctggcct actacggcgc cgtgccctcc atctcgatcg
     3541 actacggcgt catggagaaa tcggacaacg tcatcaccgt gcgcggcgcc ttcccctggg
     3601 acgacctggg ggcgtggagc gccctcgagc gggtcctccc gaacgtggac ggggtgacgg
     3661 cggtcggcgc gacgctggcc atcgactcgc ccggcgccat cctctacgcc gacgagggac
     3721 tggtggcggc gctgggcctc ccggatgtca tcgtggtgcg ggccggcaag tcggtcctcg
     3781 tcgttccgaa gtcgcgcgcc caggacgtgc gtcgcctggt gcaggccatc gaagcccgcg
     3841 acgacctgaa ggggttccga tgaggctcgg ccgcggtatc gccatcgcgc cgctcgccct
     3901 cgtcgtgctg gccgccggat gtggcggcgg acgaaaggcc caacccactg ccgagcccgt
     3961 ggcggtcacg cccgtcggcg agcaggttcc cggtccggtg ctgtcgcccc gggccgtggt
     4021 gccgctggtc agcgcggatc agctggtcga tggttatcgc gtccagctct tccagtcgac
     4081 acagcttcgt gactgcgagc agttccggga tgccgccgcc gttcgactcg acaagccggt
     4141 gtacgtggag tacctctcgc cgctttaccg ggtgcgcgcc ggagacttcc tgacccgcga
     4201 ggccgccgac gcgtggcgcg acgggcttgc ggcccagggc ttcgagcggg cggaaaccgt
     4261 gtcgtcgcgc gtgcgctcgg gcggcgggtg agaggggaga tgtcgtgagg acgaggtgta
     4321 taatcgagtc gcggaaacgc cgcggccggt acttccatcc gccaacctcg tgcggggcgt
     4381 ttccagccag gagagtgaat gccggagctg cgtaaggatc cggtcgtcgg acggtgggtc
     4441 atcatcagca ccgaccgcgg ccgccgcccc agcgatttcc acatccagac cacccccagg
     4501 gacagcaaga actgtccgtt ctgtcccggc cacgaaagcc agacgcctcc cgaggtgctg
     4561 gccgtccgcc acaacagctc gggtcccaac gcacccggct ggaaggtccg tgtcgtcccc
     4621 aacaagttcc cggccctcca gatcgaggga gatctcgaga ggcggggtga ggggctgttc
     4681 gacaagatga acggcatcgg ggcgcacgag gtgatcatcg agtccaccga tcaccacgcc
     4741 gagctctcca cgctgccggt ggacgacgtg gcgcaggtgc tcggcgccta tcgggaccgg
     4801 gtggtcgacc tgaaggggga tccccgcttc aagtacatcc tggtcttcaa gaactggggc
     4861 gaagcggccg gcgccacgct gcagcatccc cacacgcagc tcatcgccat gccgatcatc
     4921 ccgaagtggg tgtcggagga gctgaaaggg agccgcagcc acttcgagat gaaggagcgc
     4981 tgcatctact gcgacatcct gcagcaggaa acttccgacg gccggcggat ggtgacggcc
     5041 gacgagcacc acgtggccat cgtgccgttc gcgccccgat tcccgttcga aacctggatc
     5101 cttccccgga agcacagctc atccttcgag tcgatgaacg acgacgcgct ctggaacctg
     5161 gcgcgcatgc tcaaggaaac gctcacccgg atgaacgcgg cgctgaacca tccgccgtac
     5221 aacttcatca tccacacggc cccctgcaac gatccgtggc tcctctacta ccactggcac
     5281 atcgagatca tgcccaagct caccaaggtg gccggcttcg agtggggctc gggtttctac
     5341 atcaatccca cctcgccgga ggacgccgcg cgcgacctcc gcaacgccgt cccggtggaa
     5401 acggcctgat ggcccgatcc acgctggatc tgccgggcca gacgtcgcag gcctcgagcg
     5461 ggggaaagac tcccctcgag atcgcggtcg tcaccaccga gttcgcgccc ctggcgcgcg
     5521 tgggggggtt ggccgatgtc gccgcgtcgc tctccgcgga gatggcccgc ctcggggcgt
     5581 cgatcacggt ctggatgccg gcctggcgcc aggtgtggga atcgggagcc gcactccggc
     5641 cggtggcggt gccccggccc ctcgtcgttc cgctcggcgc cgaatcggtg ccggtggacc
     5701 tgctcgaggt ggtgacgccc ccgcccggcg tccgcatctt cctcatcgac tacccgccgt
     5761 tcttcgggcg aagcggcatc tatgtcgacc cggcaaccgg cgagccgtgg ctcgatcacg
     5821 accggcggct ggtgcttttc cagcgtgccg tcctcgaagc gtttcagcgg ctcgacctgc
     5881 gccccgacgt catccactgc cacgaccacc agacggggct catccccgtg tacctcaagg
     5941 tcaaggacgg actcgatccc ttcttcgcgg ggaccggcac cctgatgacg attcacaacc
     6001 tggggtacca ggggctcttc ccggcggagg tgctggagat ggcgcgcctc ccgatggacc
     6061 tgttctatcc ctccggtccg ctcgagttct ggggacgggt caacttcatg aaggggggga
     6121 ttctccacgc cgatcgcctc accacggtga gcccgcgtta cgcggaggag atccaggcct
     6181 ccgaggagat cggcttcggc ctcgaggggg tattgcgcac ccgagctgcc gacctggtcg
     6241 gcatcctcaa cgggatcgac gccgcgatgt ggaacccggc caccgatccg gtcctggccg
     6301 ccaacttcga cgccaacgac ctttcgggca aggcggcctg ccgggacgac ctcgtgaccc
     6361 ggctcggcct gaccggcgtc acggcggcca cgcccctcgt ggccatgctc tcccggctgg
     6421 tcgacgccaa gggattcgac ctcctggtgt cccagtttcc ggccatgatc gaggccgggt
     6481 ggcgcccggt gatcatcggt tcggggccac gcaagaacga ggaggcgatg gcccgcctcc
     6541 gggaccgctt tcccggccag cttcactggg ccaggagcgc ggacgacgcc ctggcccacc
     6601 ggatcgaggc cggggccgac ttctacctga tgccttcgaa atacgagccg tgcggcctga
     6661 accagatgat cagcatgcgg tacggcgccc tcccgatcgt ccgggaaacg ggggggctga
     6721 tcgacacggt ggcctcctac gacccggcca ccggtgcggg gacggggttc ggcttccggg
     6781 actacacccc cgaagggctc caggcggccc tccggctggc ccgccaggtc ttcgacgatc
     6841 cggcgcgact taccgcggtc cgccggcagg cgatgtccat ggacttctcc tggagtcgtc
     6901 cagcccgggc ctatcttgcc ctggcagagg aaattgcggc gaaacggacc cccctccgcc
     6961 gccgaaattc ctgacggccc ccgtcagttt tccgacaccc ctgtcggatt tctttctagc
     7021 aatggttcga agtcgggccc ctatttcgtg ctagaatatt tgcttactga ttacgacagc
     7081 ttttccgaca accgtcatat ggaggccggg tgccgatagc agcgagtcag atcattctga
     7141 atctatcgct gcttgcgatt gcgttccgaa cggtgattgg tgtggtgcgc catcgcaatc
     7201 cactcacgcg caccattact gtcgccaccg gcatggcctc gtcggcgtac tgcctcctcg
     7261 tcggccagct cgtcgacgtc aatcagccgg ccgcgggcgt cgacctggcc cggctcctcc
     7321 tcttcctggc cgccccggcc tgcctggcgg gccatctctt tgcccttacg gtcggtcggg
     7381 ccgacccggc cgggtcgatc cgcaggcacc ggctgtcgct cgtcgtgcag gcgctggtca
     7441 gcatggcctt cctggccttc ctgccggggc cgggattcgt tcaacgcttt gccttcagca
     7501 acggcgacta cggcctcgag ctcggcgcca tcgggcgttt ctacctggtc ttcctcctcc
     7561 tgggcaccgc cctcatcggc ttccgcctcg agaacacgct ccgggggagt gacccgactc
     7621 accgtgagag gatgcgctgg ctggccctcg gcgttttcgg aatggtcggc tacttcgtct
     7681 acctcctcac cgacggcgcc gtgaacggcc ggctcgacca ggacgtcctc ggcgcctcgc
     7741 tcatcccgat ggggctggcc gccgtactgg ccggctggac gcagctccgc ggcggtctcg
     7801 ccgacgcggg cgtgcaggtg gggcgccagg tgatcttccg ctcgctcacc ttccttttcg
     7861 tggtcggcta cgtggtggta gtgcttctcc tcggtgagct ggccgagatg ctggggatcc
     7921 gcccgagctt catcgccggg gccaccatcg tcatcggcac cgccaccgga ctgggggcgt
     7981 tcctcctctc cacgccattc cgccgaacga tgcggcggat cgtggagaag aacgtctatt
     8041 cgaaccgcct cgattaccgc acctggtggc tccgcgcctc gcacgaactg gtgtccggga
     8101 tcggcgtttc cgagctgatc ctccgcaccc agacgatgct ggaagacctc ttccactccc
     8161 ggaccatcgt catctacctc tcgcgccggc cgggggaggc ctaccagccg atgaacgccg
     8221 tgatggccgt gccgccgccc gacgtggagg cgggggaact cctcgtggcg gcgctcgagg
     8281 gcgccgccga gccgctgctg cttccggaga ggcgcgaccg gtcggtggaa ccggagctcc
     8341 tgccgatcct ggtggagaac gaagcctttc tgaaggccac cgacggccgg atcctggccc
     8401 cgctccgcac caccgagcgc ctcatcggat tcgtggtcct ggggccgcgc gccgacggtt
     8461 cggactacgg ctacgaagac cgctcgctcc tcgagacagt cacgctgcag ctggccggcg
     8521 ccatccgcgg cgcctggctc accgaggacc tcggcgcggc ccgcgagatg gaagtcttca
     8581 gccagtgggc gggcgtggtg gtgcacgacc tcaagaactt cgtctcgccg ctcaagctct
     8641 tcgtccagaa cgcccgccgc cacctccaca acgcggaatt ccgcgagcag gccatcgacg
     8701 acatccaggg ggtggtcgag aagatcgacg gcgccatcca gcgcctcacg gccctgaagc
     8761 acggcgagga gatcgcgctg gccccgaccg acctcgaatg gatcctcgac agcgtgctca
     8821 cccggagcgg cgtctcgatg cgtcccggct acagcgtggc ccgcaacttc gggggagtac
     8881 cctcggtggt ggccgaccag ctcctcatcg accgcgtgct gaccaacctc gtcaccaatg
     8941 ccatggaggc catgcccgag ggg
//
LOCUS       DASVOD010000497         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_344431, whole genome shotgun sequence.
ACCESSION   DASVOD010000497 DASVOD010000000
VERSION     DASVOD010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344431"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..851
                     /locus_tag="VF720_11175"
     CDS             243..851
                     /locus_tag="VF720_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX7879964.1"
                     /translation="MKLVTRSLLAVLALVASASLAAAAPATWKIDPAHTEAGFEVRHF
                     FSKVHGVFHDIEGTITFDEADPAAIKVDASVKVASVDTGNEKRDGHLQSADFFDAEKN
                     PTLTFKSTKVAQAGKGKYKITGDLTMRGVTKSVVFDAEFLGSSAVSVGGQSWGSKAGF
                     SATTVVNRKDYGINWNKALDNGGVMVDDMVTIVLNVEADKVQ"
     gene            complement(957..1466)
                     /locus_tag="VF720_11180"
     CDS             complement(957..1466)
                     /locus_tag="VF720_11180"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7879965.1"
                     /translation="MRLRDSLLTLLCSVALASTAAAQTTYVGVLTGGSEVPPVVTAAT
                     GNATVVLDASQTQLSISVQFQNLTSAYTASHIHGPAPAGTNAGVKWGFVGTTAGWVFG
                     AGNQSGTITNYLVTGVTATDVTNLNAGNFYVNIHSSTFPGGELRAQLGSAPVPALKST
                     WSRVKSLFR"
BASE COUNT          305 a          645 c          673 g          286 t
ORIGIN      
        1 tgccggaggg ttgacttggg gctgggggat tcggtgatag agtactcggg cgtttgttgc
       61 gtgtttcaac atgtacatgt cagccaccgg tcaagactct gcgccggaac cccatgggct
      121 cccatccaac gcccagattc gcggcaccgg ccgcggaacg actcgacccg gaatcacgca
      181 gtcctcggac tcccccgatg tccacggccc tgctcgtcgc tgtcccgacc aaggagacgt
      241 ccatgaagct cgtgacccgc tcgctgctcg ccgtcctcgc tctcgtcgcc tcggcctcgc
      301 tcgcggccgc ggcgccggcc acctggaaga tcgacccagc gcacaccgag gccggcttcg
      361 aggtccgaca cttcttctcg aaggtgcacg gcgtgttcca cgacatcgag ggcacgatca
      421 cgttcgacga ggccgacccg gccgcgatca aggtcgatgc ctcggtcaag gtcgccagcg
      481 tcgacaccgg caacgagaag cgtgacggcc acctccagag cgcggacttc ttcgacgccg
      541 agaagaaccc gacgctgacg ttcaagagca cgaaggtcgc gcaggccggc aagggcaagt
      601 acaagatcac cggcgacctc accatgcgcg gcgtcaccaa gtcggtcgtg ttcgacgccg
      661 agttcctcgg ctcctcggcc gtcagcgtcg gcgggcagtc gtggggctcg aaggccggct
      721 tctcggccac gacggtcgtc aaccgcaagg actacggcat caactggaac aaggcgctcg
      781 acaacggcgg cgtcatggtc gacgacatgg tcacgatcgt gctcaacgtc gaggccgaca
      841 aggtccagta gcggccagcg cggtccggat cgcgatggac gagcagcacg aaggccccgg
      901 agcgacgccc cggggccttc gtgttccttc ccggcaccgc gcggcgccgg tgcgggctag
      961 cggaacagcg acttcacgcg gctccacgtg ctcttgagtg cgggcacggg cgcggagccg
     1021 agctgcgcgc gcagttcgcc acccgggaac gtggaggaat ggatgttcac gtagaagttg
     1081 ccggcgttga ggttcgtcac gtcggtcgcc gtgacgccgg tcacgaggta gttcgtgatc
     1141 gtgccgctct ggttcccggc gccgaacacc cagccggccg tcgtgccgac gaagccccac
     1201 ttgacgccgg cgttcgtgcc ggccggcgcg gggccgtgga tgtgcgaggc ggtgtaggcg
     1261 gaggtcaggt tctggaactg gaccgagatc gacagctgcg tctgcgacgc gtcgagcacg
     1321 acggtggcgt tcccggtggc ggcggtgacg acgggcggga cctcggagcc accggtgagt
     1381 acgccgacgt aggtggtctg ggccgccgcc gtggatgcaa gggcgacgct gcagagcagg
     1441 gtgaggagcg agtcgcgaag acgcatgggg ggcctcctgg ggatggacag ggacggcacg
     1501 cccggtgggg tgcggcggtc cgtgcggacg cagccgtacg gctgcatcct ggtggcagtg
     1561 tgcgccgcgc gcagcgggct cctcaactgc aatcgtgcgc cggggcaccc ggcccggtgg
     1621 atttcaccgt cgatggaacc acttcccgca gtgcgcagcg ggtgatccct ccggccgcac
     1681 atggcgccgg ggggccgcgc gggagactgc acgagaagct gccgctcggg ccaattcgat
     1741 actgcgctgc acgtgccggg agcggataat cgtccgtggt ccccacgccg ccccccctgc
     1801 acgaggtccc tcatgaagcg tgtgctgccc accctgatgc tcatagccgc cctgctgcgg
     1861 agcctgcccg cgacggccgc ccccgccggg cactgcgccc cggcgacga
//
LOCUS       DASVOD010000498         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_85835, whole genome shotgun sequence.
ACCESSION   DASVOD010000498 DASVOD010000000
VERSION     DASVOD010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85835"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2366
                     /locus_tag="VF720_11185"
     CDS             <1..>2366
                     /locus_tag="VF720_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX7879966.1"
                     /translation="AGRTHVHKLTGSSRTLLAAALHEKTKRPVLYVAADGERAEAARD
                     DLEFWLDRERVVHFAQHDQPPYDTQTPHLEPTAARLDALVRLATAPDSVGVVVAAAGG
                     LGQNIVGPDVLVDSLLVFKVGATLDLEKVTERLVTLGYERTPTVEDVGQFSRRGGILD
                     VYSPGNPNPIRVEIDDDFTASMREFDVIHQRSIRPLDVAHIIPTHELALDPDRLELAL
                     LELEETKPDLAAELREQFETDLYPPGVEKLLPYLLQNPGSLMRYLRPGTIVVLEEPDL
                     VFDGVDAQWNEATAAYEKLHGDQAHLAKPHDLYRSRETVAADLAAFDHVGLSALTAEA
                     PGAGLVTIRSAPPDNFGRNLEMWRKYLVGLHEAGETTHLLCDNPGQKNRLDELLLESG
                     TPVHLEVGILAEGFRLPDLRVAVLTDHEFFGRSRRARRARKFKSGFGLKEITSLRPGT
                     YIVHIEHGIGIYRGISRLEINGQTTDCLLLEYGSGDRLYLPVDQLDRVQKFSADEESR
                     PSLSRIGGTGWARTKEKAKKAIKEMAGELIALYARRRALPGHPFGPDTVWQQELEGTF
                     PFDETPDQLKAVEDVTKDMESATPMDRLVCGDVGYGKTEVAVRAAFKAVMEGKQVAVL
                     VPTTILAQQHTNTFRERYAGFPVIVELLSRFRSPKEQQEVLKGVAAGTVNVVIGTHAL
                     LAARVQFKELGLVIVDEEQRFGVAHKEKLRKMRTQVDVLTLTATPIPRTLNMAMLGAR
                     DISTITTPPRDRLPIHTEIVEFEDDLIQDALLREADRGGQSFFVHNRV"
BASE COUNT          405 a          823 c          799 g          339 t
ORIGIN      
        1 gggccgggcg gacgcacgtc cacaagctca ccggctcctc gcgcacgctg ctggccgcgg
       61 cgctgcacga aaaaacgaag cggccggtgc tctacgtggc tgccgacggc gaacgggccg
      121 aggccgcgcg ggacgacctc gagttctggc tcgaccgcga gcgggtcgtg cactttgccc
      181 agcacgacca gcctccttac gacacgcaga cgccccatct cgagcccacc gccgcacgcc
      241 tcgacgcgct ggtgcgcctg gccaccgcgc ccgacagcgt cggcgtggtg gtggccgcgg
      301 ccggcgggct cgggcagaac atcgtcggtc ccgacgtgct ggtcgattcc ctcctcgtct
      361 tcaaggtggg ggccacgctc gacctcgaga aggtgacgga gcgactggtc acgctggggt
      421 acgagcgcac gcccacggtg gaagacgtgg ggcagttcag ccggcgcggc ggcatcctcg
      481 atgtgtacag ccccggcaac cccaacccga tccgcgtgga gatcgacgac gacttcacgg
      541 cctcgatgcg cgagttcgac gtcatccacc agcgctcgat ccgaccgctc gacgtggcgc
      601 acatcatccc cacgcacgag ctggcgctcg accccgatcg gctcgaactg gcgctcctcg
      661 agctggagga gacgaagccc gacctggccg ccgagctgcg cgagcagttc gaaacggatc
      721 tctatccgcc gggcgtcgag aagctgctgc cgtacctgct gcagaacccg ggctccctga
      781 tgcgctacct ccgccccggt acgatcgtgg tactcgaaga gcccgatctc gtgttcgacg
      841 gcgtggacgc ccaatggaat gaggccacgg cggcctacga aaagctccat ggcgaccagg
      901 cgcacctggc caagccgcac gacctttacc ggagccggga gacggtggcc gcggacctcg
      961 cggcgttcga ccacgtgggg ctgtcggcgc tcacggccga ggcgcccggc gcggggttgg
     1021 tcacgattcg ttcggcgccc cccgacaatt tcggtcgcaa tctcgagatg tggcggaagt
     1081 atctcgtggg cctccacgag gccggcgaga cgacgcacct gttgtgcgac aacccggggc
     1141 agaagaaccg gctcgacgag ctgctgctcg aaagcggcac gccggtgcac ctcgaggtgg
     1201 ggatcctggc ggaggggttc cggctccccg acctcagggt ggcggtgctc accgatcacg
     1261 aattcttcgg tcgctcccgt cgtgcccgcc gggcccggaa attcaagtcc ggtttcggcc
     1321 tcaaggagat caccagcctc cggccgggca cctacatcgt gcacatcgag cacggcatcg
     1381 gcatctaccg cggcatctcg cggctcgaga tcaacgggca gaccaccgat tgcctcctcc
     1441 tcgagtacgg ctcgggcgat cgcctctacc tgccggtcga ccagctcgac cgcgtgcaga
     1501 agttctcggc cgacgaggag tcccgcccaa gcctctcccg gatcggcggc accggctggg
     1561 ccaggaccaa ggagaaggcc aagaaggcca tcaaggagat ggccggcgag ctcatcgcgc
     1621 tctacgcgcg gcggcgcgcg ctgcccgggc acccgttcgg ccccgacacg gtgtggcagc
     1681 aggagctgga aggaacgttc cccttcgacg agacgcccga ccagctgaaa gccgtggagg
     1741 acgtgacgaa ggacatggag agcgccacgc cgatggaccg cctggtctgc ggcgacgtgg
     1801 gatacggcaa gaccgaggtg gccgtgcgcg ccgccttcaa ggccgtgatg gaggggaagc
     1861 aggtggcggt gctggtgccc accaccatcc tggcccagca gcatacgaat acgttccggg
     1921 agcgctacgc cggcttcccg gtgatcgtgg agctcctctc gcgtttccgc tcgccgaagg
     1981 agcagcagga ggtgctgaaa ggggtggccg cgggcaccgt gaacgtggtc atcggcacgc
     2041 acgcgctgct ggcggcccgc gtgcagttca aggagctcgg cctcgtgatc gtggacgagg
     2101 aacagcgctt cggcgtggcg cacaaggaaa agctgcggaa gatgcgcacc caggtggacg
     2161 tgctcacgct caccgccacg cccatcccgc gcacgctcaa catggccatg ctcggcgccc
     2221 gcgacatctc caccatcacc acgccgccgc gcgaccgtct gccgatccac accgagatcg
     2281 tggagttcga ggacgacctg atccaggacg ccttgctgcg cgaggccgac cggggcgggc
     2341 agtcgttttt cgtgcataac cgagtc
//
LOCUS       DASVOD010000499         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_762320, whole genome shotgun sequence.
ACCESSION   DASVOD010000499 DASVOD010000000
VERSION     DASVOD010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762320"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1495
                     /locus_tag="VF720_11190"
     CDS             <1..1495
                     /locus_tag="VF720_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7879967.1"
                     /translation="GQLVALQYTRNDIDFRRGTFRIRGDVLEVRPAYEEHAVRIELFD
                     DDIESLSIVDPLTGRTLTKLDRVAVYPAKHFVTERPKLEAAVRNIKHELRDRLAEFNA
                     NQKFLEAERLKSRCEYDMEMMMELGYCPGIENYSRWIDGRQPGQRPHCLLDYFPKGKD
                     WLLIIDESHVTIPQIGAMYEGDRNRKQTLVDFGFRLPSALDNRPLKFGEFETLVPPTI
                     FVSATPSEFELRKSEGVVVEQIIRPTGLIDPAIVLRPIAGQIDDLMEEIRKTVARQER
                     VLVTTLTKRMSEDLSDFLLGAGIKVAWLHSDIDAIARMEILRGLRLGEYDVLVGINLL
                     REGLDLPEVGLVAILDADKEGFLRSETSLIQTAGRAARNVAGRVILYADQMTGSMKRA
                     IDEMNRRREKQMAWNTEHGITPQSIVKSIDEVMLSTAVADATPEKDDVDPSVALLSHL
                     SATDRPEMIRVLEREMRQAARALEFERAASLRDKIEELKLELKLESR"
     gene            1492..2403
                     /gene="nadC"
                     /locus_tag="VF720_11195"
     CDS             1492..2403
                     /gene="nadC"
                     /locus_tag="VF720_11195"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEX7879968.1"
                     /translation="MIATGPGGPPPDFGAVREAAWPLIALALAEDLGERGDVTSLATI
                     VPDVAVAAHLVARAEGVLAGLDVARTVLMAVDGAAGFSAEARDGDRLSPGMIVATVTG
                     SARSLLSAERTMLNFVQRLSGVASQVARYVAAIEGTGVRVLDTRKTTPGWRVLEKYAV
                     AIGGGVNHRMGLFDLILIKDNHAMAAGGVGPAVAAALASRPEGVRIEVEVRTLADIDA
                     ALAYPVDGLLFDNMNPASIDAAVARVARLVAGRPASRPFIEVSGGITLATIRNHARPG
                     VDFISVGAITHSAPALDLALDIVEDQG"
     gene            2400..>3024
                     /locus_tag="VF720_11200"
     CDS             2400..>3024
                     /locus_tag="VF720_11200"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX7879969.1"
                     /translation="MTCIRGSAVFADDIRAVVQRARHVTRVHHHDVVGSTNAEALELA
                     RAGAPHGTLVIADRQTAGRGRQGRRWESPPGVGLWFSWVLRPELELARGFLVTALGAL
                     AISDAVGRLTGRAATVRWPNDVLVGRSKLAGVLAEARGQGTRLEVVVLGIGVNVGQRA
                     ADFPEELAGIATSMSIEAGRPVDRVLAFQSIVESFEQGYDQMQRDGGV"
BASE COUNT          479 a          983 c         1090 g          472 t
ORIGIN      
        1 ggggcagctg gtggccctgc agtacacccg caacgacatc gacttccggc gcggcacgtt
       61 ccgcatccgc ggggacgtgc tcgaggttcg gcccgcgtac gaagagcacg cggtgcggat
      121 cgagctgttc gacgacgaca tcgagtcgct ctcgatcgtc gacccgttga ccgggcgcac
      181 cctgacgaag ctcgaccggg tggccgtcta tccggccaag cacttcgtga ccgagcgtcc
      241 gaagctcgag gcggcagtgc ggaacatcaa gcacgagctg cgcgaccggc tggccgagtt
      301 caacgccaac cagaagttcc tcgaggccga acggctcaag agccggtgcg agtacgacat
      361 ggaaatgatg atggagctgg ggtattgccc gggcatcgag aactacagcc ggtggatcga
      421 cggccgtcag cccggccagc gaccgcactg cctgctcgac tacttcccga aggggaagga
      481 ttggctcctg atcatcgacg agtcgcacgt cacgatcccg cagatcgggg ccatgtacga
      541 aggggatcgg aatcgcaagc agacgctggt cgatttcggc ttccgcctgc cgtcggcgct
      601 cgacaaccgg ccgctcaagt tcggcgagtt cgaaacgctc gtgccaccca cgatcttcgt
      661 gtcggccacg ccgtccgagt tcgagctccg gaagtcggag ggcgtggtgg tcgagcagat
      721 catccggccg acgggcctga tcgacccggc catcgtgctg cggccgatcg cgggccagat
      781 cgacgacctg atggaggaaa tccgcaagac ggtggcgcgc caggagaggg ttctcgtgac
      841 gacgctcacg aaacgcatgt ccgaggacct ctccgacttt ctcctcggtg cgggcatcaa
      901 ggtggcctgg ctccactcgg acatcgacgc cattgcccgc atggaaatcc tccgaggcct
      961 gcggctcggc gagtacgacg tgctggtcgg catcaacctg ctgcgggaag ggctcgacct
     1021 cccggaagtg ggactggtgg ccatcctcga tgccgacaag gaagggttcc tccgctccga
     1081 gacgagcctc atccagaccg ccggccgggc cgcgcgcaac gtggccggcc gcgtgatcct
     1141 gtatgccgac cagatgaccg gctcgatgaa gcgggccatc gacgagatga atcgccgccg
     1201 cgagaagcag atggcctgga acaccgagca cggcatcacg ccgcagtcga tcgtgaagtc
     1261 gatcgacgag gtgatgctct ccaccgccgt ggccgatgcc acgccggaga aggacgatgt
     1321 cgatccctcg gtggcgctcc tctcccatct gtcggccacc gaccggccgg aaatgatccg
     1381 ggtgctggag cgcgagatgc ggcaagcggc ccgtgcgctg gaattcgagc gggccgccag
     1441 cctcagggac aagatcgagg agttgaagct cgagctgaaa ctggagtcgc gttgatcgcc
     1501 acggggcccg gcggtccgcc gcctgacttc ggggcggtgc gggaggcggc ctggcccctc
     1561 atcgcgctgg cgctggccga ggacctgggt gagcgggggg atgtgacgtc gctcgccacg
     1621 atcgtgccgg acgtcgcggt ggccgcacac ctggtggccc gcgccgaggg agtgctggcg
     1681 gggctcgacg tggcgcgcac ggtcctcatg gcggtcgacg gcgctgccgg gttcagcgcg
     1741 gaggcccgag atggcgaccg cctgtctccc ggaatgatcg tcgctacggt aaccggttcg
     1801 gcacggtcac ttctctccgc ggagcgaacg atgctgaact tcgttcagcg gctctcgggt
     1861 gtggccagcc aggtggcgcg ttacgtggcg gccatcgaag gcaccggtgt gcgggtgctc
     1921 gatacccgga agaccacgcc ggggtggcgc gtcctcgaga agtacgcggt ggccatcggt
     1981 gggggcgtca atcaccggat ggggctgttc gacctgatcc tcatcaagga caaccacgcc
     2041 atggcggccg gcggcgtcgg gcccgcggtg gcggccgcgc tggcgtcccg gccggaggga
     2101 gtccggatag aggtggaagt gcgcacgctc gccgacatcg acgcggcgct ggcgtacccg
     2161 gtcgacgggc tcctcttcga caacatgaac cccgcgtcga tcgatgcggc cgtggcccgc
     2221 gtcgctcgac tggtggccgg tcggccggcc tcgcgtccgt tcatcgaggt gtcgggcggc
     2281 atcacgctgg ccaccatccg gaaccatgcg cgtcccgggg tcgatttcat ctcggtgggg
     2341 gcgatcacgc attccgcgcc ggcgctcgac cttgccctgg atatcgtcga ggaccagggg
     2401 tgacgtgcat ccgaggcagc gccgtgtttg ccgacgacat ccgcgcggtg gtgcagcggg
     2461 cccgccatgt cacccgcgtg catcatcacg atgtggtggg ctcgaccaac gccgaggcgc
     2521 tcgaactcgc gcgggccggg gcgccgcacg gaacgctggt catcgccgac cggcagacgg
     2581 ccgggcgcgg ccggcagggg cggcggtggg aatcgccgcc gggagtcggg ctctggttct
     2641 cctgggtgct ccggccggag ctcgagctcg cccgcggttt tctcgtcacc gcgcttggcg
     2701 cactggccat atcggacgcg gtcgggcgcc tcaccggccg ggcggccacg gtgcgctggc
     2761 ccaacgacgt cctggtgggt cggagtaaac tggccggggt ccttgccgag gcgcgaggac
     2821 agggaacgcg cctcgaggtg gtggtgctgg ggatcggcgt gaacgtgggg cagcgcgccg
     2881 ccgacttccc ggaggaactg gccggtatcg ccacttccat gagcatcgag gccggccggc
     2941 ccgtcgaccg tgttctcgcc ttccagtcca tcgtcgaatc gttcgaacag ggttacgatc
     3001 agatgcagcg ggacgggggc gtcc
//
LOCUS       DASVOD010000500         2210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_241672, whole genome shotgun sequence.
ACCESSION   DASVOD010000500 DASVOD010000000
VERSION     DASVOD010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241672"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /gene="secA"
                     /locus_tag="VF720_11205"
     CDS             complement(<1..735)
                     /gene="secA"
                     /locus_tag="VF720_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926757.1"
                     /note="functions in protein export; can interact with
                     acidic membrane phospholipids and the SecYEG protein
                     complex; binds to preproteins; binds to ATP and undergoes
                     a conformational change to promote membrane insertion of
                     SecA/bound preprotein; ATP hydrolysis appears to drive
                     release of the preprotein from SecA and deinsertion of
                     SecA from the membrane; additional proteins SecD/F/YajC
                     aid SecA recycling; exists in an equilibrium between
                     monomers and dimers; may possibly form higher order
                     oligomers; in some organisms, there are paralogous
                     proteins that have been found to be nonessential but do
                     function in secretion of a subset of exported proteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX7879970.1"
                     /translation="MGSATKLATRLFGTKHDKDRKRLEPLIGEINEAFEGLAGLSDDD
                     LRARTATWQGEFKAIWNDPELSPEEQEAAVKEHLESILPEAFAVVKDACRRQVGKSWP
                     VVGIETRWEMIPYNVQLLGGIVLHEGKIAEMATGEGKTLVAVLPLYLNALPGKGCHLV
                     TVNDYLARRDAEWVGSVLKFLGLTVGVIQHDQSPDVRRAQYDADVTYGTNNEFGFDYL
                     RDNMAVRMEDRVQRGFWYTIVDEVDSV"
     gene            complement(957..1163)
                     /locus_tag="VF720_11210"
     CDS             complement(957..1163)
                     /locus_tag="VF720_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007277145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX7879971.1"
                     /translation="MATRGKVKWFNETKGYGFISRDEGQEVFVHFSAIQGDGYRTLRE
                     GQEVEFEVQDGEKGPQAMHVIQLV"
     gene            complement(1269..>2210)
                     /locus_tag="VF720_11215"
     CDS             complement(1269..>2210)
                     /locus_tag="VF720_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7879972.1"
                     /translation="ALGGAVTGTPLVLNDRVVVAGRDGRLCALDRRDGRILWSQSARK
                     GFGGGAVGNDSIAAVAGFDGVLRLLAPADGREVGRLDTESRIASPPVFTAEGFVVAGA
                     YDRTLYALDPSNGRSPWKARLGGILWASPAAGRKYIYAPDLDGVLTAVNSRWGSVLWK
                     FKAGGEIYSSPAVADERVVFGCEDGSIYAVTAEEGRLLWRTPVGKPVSGGARIDEGRA
                     YLGDDGGILHALDAATGRELWRYPAGGAIKSTPVVDTGTVLLSAHDGALHAVDAATGA
                     RIGRFPIGSPIAAAPAMSGDTLFVGTHDGRVVALRLR"
BASE COUNT          365 a          760 c          704 g          381 t
ORIGIN      
        1 cacggagtcg acttcgtcga cgatcgtgta ccagaagccg cgctggaccc gatcttccat
       61 ccgcacggcc atgttgtcgc gcaggtagtc gaagccgaat tcgttgttgg tgccgtaggt
      121 gacgtcggcg tcgtactgcg cgcgccgcac gtcggggctc tggtcgtgct ggatgacgcc
      181 cacggtgagg ccgaggaact tgagcacgga gcccacccac tcggcgtcgc ggcgggccag
      241 gtaatcgttg acggtgacga ggtggcaccc cttccccggc agcgcgttca ggtagagcgg
      301 cagcacggcc accagggtct ttccttcgcc ggtggccatc tcggcgatct tcccctcgtg
      361 gaggacgatg ccgccgagga gctgcacgtt gtaggggatc atctcccagc gcgtctcgat
      421 gccgacgacc ggccagctct ttccgacctg ccggcggcag gcgtccttca ccacggcgaa
      481 tgcttcggga aggatgctct ccaggtgttc cttgaccgcg gcttcctgtt cctcggggga
      541 gagctcgggg tcgttccaga tggccttgaa ttccccctgc caggtggcgg tccgggcccg
      601 gagatcgtcg tccgacaggc cggccaggcc ctcgaaggcc tcattgattt cgccgatgag
      661 cggctccagc cgcttccggt ccttgtcgtg cttggtgcca aaaagacggg tggcgagctt
      721 ggtcgccgaa cccataaacc ctgtagccat ttgagcttcc ttgtgggatt cgtccgccgg
      781 agggccgtct cggccactcc catcatgttg tcgggacgcg ccacgctatc agacgggacg
      841 agcctccggc aaggcggggc gggtatacgc ccggaacgat cattggtttc gggaagggca
      901 aaaaaagtcg cccgcctcgg ggcgggcgac cacttaactt atttcataac agtaaattaa
      961 accagttgga tgacgtgcat ggcctggggt cctttctccc cgtcctggac ctcgaactcg
     1021 acttcctgcc cctcgcggag agtccggtaa ccgtcgccct ggatagcgga gaaatgcacg
     1081 aaaacctcct gcccttcatc gcgactgatg aaaccgtaac ccttggtttc gttgaaccac
     1141 ttcaccttgc cccgcgtggc cattgttcga tcctcccacg cccgggggcc gtcccgggct
     1201 tgggttcacc ttagtaatgg gaatcgcccg gtgtcaagca aagatcggct ccccaaaggg
     1261 gaggattcct accggagccg gagggccacc acgcgtccgt catgcgtgcc gacgaagagg
     1321 gtgtcgccgc tcatcgccgg ggctgcggct atgggagaac cgatcggaaa gcggccgatc
     1381 cgggccccgg ttgccgcgtc cacggcatgc agcgccccgt catgggccga cagcagaacc
     1441 gtgccggtat cgacgaccgg agtggacttg atggcgccgc cggccgggta gcgccagagc
     1501 tcgcgcccgg tggcggcgtc gagggcatgg aggatcccgc cgtcatcacc gaggtaagcc
     1561 cggccctcgt cgatccgcgc accgccggac acgggcttcc cgaccggcgt ccgccagagg
     1621 agccgccctt cctcggcggt gacggcgtag atcgatccgt cctcgcaacc gaacacgacc
     1681 cgctcatcgg cgacggcggg tgatgagtag atttcgcccc ccgccttgaa cttccagagg
     1741 acgctccccc atcggctgtt caccgccgtc aggacgccat cgaggtcggg cgcgtagata
     1801 tacttccgac cggccgccgg cgaggcccac aggatgccgc ccagccgcgc cttccacggc
     1861 gagcgaccat tgcttggatc cagagcgtag agcgtccggt catacgcccc tgccaccacg
     1921 aacccttccg ccgtgaagac cggcggtgag gcgatccggc tctcggtgtc gaggcgtccc
     1981 acttcgcgac cgtcggcagg cgccagcagt cgcaggacgc cgtcaaaccc ggccaccgcg
     2041 gcgatcgagt cgttgccgac cgcaccaccc ccgaatcctt tccgggccga ctgcgaccag
     2101 aggatccttc catccctccg gtcgagcgcg cacaaccggc cgtcgcgtcc cgccacgacg
     2161 acccgatcgt tgaggaccag cggagtcccg gtaacggcac cgccgagggc
//
LOCUS       DASVOD010000501         5910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1027059, whole genome shotgun sequence.
ACCESSION   DASVOD010000501 DASVOD010000000
VERSION     DASVOD010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027059"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2468)
                     /locus_tag="VF720_11220"
     CDS             complement(<1..2468)
                     /locus_tag="VF720_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879973.1"
                     /translation="MNLGRLGLVFREELGHAARRPIFWFLLVLLAFSLWGLSAGSVRI
                     ASGDSQVGGTKAFITSEFAVTQIASFLVLLLYAFFVAVAAGMAVIHDEEHQVGAILHA
                     TPLTPAEYVYGKFAGVFAAVCGVLALHVLFSMFFNHVVPSEVAEEVRGPFNLLNYLKP
                     ALFLSLPTLFFLTATAFAVGTATRKPILVFVLPVITILVCGFFLWDFSPSWLSPAWNK
                     FLMVIDPSGFRWLNETWLKVDKGVAFYNHQPVGYDVTFWLNRLWQVSAGIIALVLSER
                     AFAGSLKGATVRKGGRARSGEDKPAPALDATRRSAPLSALGMEARRPGFLEGMRTIGR
                     AEVRELFSQPGLYLFIPLILLQVIGDNLFAIGAFDTPLLATNGTMAVGTFNTLTLLST
                     LLLLFYTVEGMERERATRLDPIFRATPVSTVSMLVGKTIAAVMVAMVILAAVLLSAII
                     IILVQGKVTFNPLPFLLVWGLLLLPTLLVWITFIVALHTLVRNRYLTYALGLAVLIVT
                     GVCQMTGRMNWVGNWDMWSTLQWSDMGLFELNRSAIILNRVMVLGLAIFFVAVTLRIV
                     TRRERDASRLGHRLSPGPLLKGGLRLLPFAIVPIVAGIFLFLQVWNGFQGKPALKKQK
                     DYWRRNLATWKNAPLPDLVHVDLDMKLEPARRTFDLTGSYRLVNKLDEPLRQIPITGS
                     YTWTNIRWTLNGDSTKPDDRLKLFIFTPKEPLAPGDSLTIGFAYGGSWPGGVTKNGAG
                     NMTFLLPSGVVLTSFEPSFAPIVGYQESIGIDKDNRYDARDYPDDFHKGVTKAALGPQ
                     MPFTTHIRLNGPDEFRYNSVGTLLS"
     gene            complement(2465..3394)
                     /locus_tag="VF720_11225"
     CDS             complement(2465..3394)
                     /locus_tag="VF720_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019398079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7879974.1"
                     /translation="MLSVRRLVKIYPGPVAALQGIDLDVGKGMFGLLGPNGAGKSTFM
                     KILAGLLEPTSGTVTLGGEDILARPERLWNRLGYLPQEFGFFPHLTGEQMLRYLLELK
                     GVEAPGGLKRLVAELLDRVNLTFAAKRKVKGYSGGMRQRLGIAQAIAGNPELIIVDEP
                     TAGLDPEERHRFYRLLAELAEQRIVLLSTHIVEDVSVLCPRFAVIKGGQLLSVTTPGA
                     ARTEMTGRVFEVAVTAEELAALERRHTVLQAILFEGKNRARLYVPDDLPPVGAEPVTA
                     TLEDAYLLLMRRADQADQAPRGDIGLLAAGSPS"
     gene            3507..3995
                     /locus_tag="VF720_11230"
     CDS             3507..3995
                     /locus_tag="VF720_11230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7879975.1"
                     /translation="MSDPNSIEPGPSETTPVTVVIGPAPFNAHEGIAELLRSAALPVP
                     DPEDSPVQMMAAFAGPHLIGCIGWEQYGASALVRSLAVLEDVRGEGVGTALVGALVAV
                     LKGIGVDEAWLLTTDAAGWFERHDFERTDREAIPEAVRVAREIRLPCCATAQVMRRRL
                     DS"
     gene            complement(3992..>5910)
                     /locus_tag="VF720_11235"
     CDS             complement(3992..>5910)
                     /locus_tag="VF720_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879976.1"
                     /translation="RACNDLLFLALVMGSLNLLFRRDAGSRGDAGLRGEGSVLRDWGV
                     AGALGGLAWMTRYNGSVLLPAALLTALLLFGASRRMLSAAGAWVGAWIVTCLPWLLFV
                     MAKTGTPFWNKAYENIAIEVLSDDANRAQTTGFMSATNIASLKDVWDVDPGLFLKAMA
                     SNVVDHLRDDATKLVGVPWAIVAVGGLLLGWQSWRNRRALAFLATGVLTYLSLLPVFY
                     NERFMLPLLPYWGAMAGGTAVWLGSYRPAAAGTPRAGGKGRAPKPGAKAGPSGIGRLL
                     PPLLGAFGVLTIVLSIAGFRLATDPKRFGGMPGEYVTLLADARRRNVPLGPEHPIAAR
                     KPHFPYMAGSPTVGIPAGSIEQLRSSGAHYLLVSGSEVAVYPSLASIWIPKSAAEIPQ
                     GLALAGGGTWRRSSGDVMAATLYAVLDPLPYVKRESSPFKKGVPVPDGYSRVDFNRLL
                     LGEWILKSGALRDPDQFFRLMSPAARELPRTRLWRGAAAIRRGDLPEAERLYRAVAEG
                     DPANRAAWLGVAATSYLAGRQGEHAAAINRAFGIALSDSLPVDSLKAEGDERLRRMET
                     PQSLAVATAMLATNPTSDWALKSAAYSLQALFRPAESRRMAEAVLLHNPEDREAQTIL
                     NTLEMDGGVPGMKR"
BASE COUNT         1022 a         1935 c         2052 g          901 t
ORIGIN      
        1 gacaggagcg tgccgaccga gttgtagcgg aactcgtcgg gaccgttcag ccggatgtgg
       61 gtggtgaacg gcatctgcgg gccgagcgcg gccttcgtca ctcccttgtg gaagtcgtcg
      121 gggtaatcgc gcgcgtcgta acggttgtcc ttgtcgatcc cgatcgactc ctggtagccc
      181 acgatgggcg cgaacgaggg ctcgaagctg gtgagcacca cgcccgacgg gagaaggaag
      241 gtcatgttcc cggcgccgtt cttcgtcacg ccgccgggcc agctccctcc gtacgcgaag
      301 ccgatggtga ggctgtctcc gggggccagc ggctccttcg gcgtgaagat gaagagcttc
      361 agccggtcgt ccggcttcgt cgagtcgccg ttgagcgtcc agcggatgtt ggtccaggtg
      421 tagctcccgg tgatcggtat ctggcgaagc ggctcgtcca gcttgttcac cagccggtag
      481 gagccggtca ggtcgaaggt gcgacgggcc ggctcgagct tcatgtccag gtcgacgtgc
      541 acgaggtcgg gaaggggcgc gttcttccag gtggccaggt tccgccgcca gtagtccttc
      601 tgcttcttga gggccggctt cccctggaag ccattccaga cctgcaggaa caggaagatg
      661 ccggccacga tcgggacgat cgcaaagggc aggaggcgga gccccccttt caggagcggg
      721 ccgggagaaa ggcgatggcc caggcgggag gcgtcgcgct cgcggcgggt cacgatccgg
      781 agcgtcaccg ccacgaagaa gatggcgagg cccagcacca tcacccggtt caggatgatg
      841 gcggagcggt tcagctcgaa cagccccatg tcgctccact ggagcgtgga ccacatgtcc
      901 cagttgccta cccagttcat cctccccgtc atctggcaga cgccggtcac gatgaggacg
      961 gccaatccga gcgcgtaggt caggtaccgg ttccgcacga gcgtgtgcag ggccacgatg
     1021 aacgtgatcc agaccaggag ggtggggagg agcaggaggc cccacacgag aaggaagggg
     1081 agcggattga aggtcacctt cccctgcacg aggatgatga tgatcgccga caggagcacc
     1141 gcggccagga tgaccatcgc caccatcacc gccgcgatcg tctttcccac cagcatcgag
     1201 accgtggaga cgggcgtggc ccggaagatc gggtcgaggc gggtggcccg ctcccgttcc
     1261 atcccttcca ccgtgtagaa gaggagaagg agcgtcgaga gaagcgtgag cgtgttgaag
     1321 gtgcccacgg ccatcgtccc gttggtggcc aggaggggcg tgtcgaaggc gccgatggcg
     1381 aagaggttgt cgccgatcac ctgcagcagg atgagcggga tgaagaggta gaggccgggc
     1441 tggctgaaaa gctcgcgaac ttcggcgcgg ccgatcgtac gcatgccttc caggaagccc
     1501 ggccggcgcg cttccatccc gagcgcggag aggggggccg atcgccgcgt ggcgtcgagc
     1561 gccggggccg gcttgtcttc gccgcttcgt gcccttcccc ctttcctgac ggtggccccc
     1621 ttcaggctcc cggcgaaagc gcgctccgag aggaccagcg cgatgatccc ggcggagacc
     1681 tgccagaggc ggttgagcca gaaggtgacg tcgtaaccca ccggctggtg gttgtagaac
     1741 gccactccct tgtccacctt cagccacgtc tcgttgagcc agcggaagcc cgagggatcg
     1801 atcaccatca ggaacttgtt ccaggcgggc gagagccacg aaggggaaaa gtcccagagg
     1861 aagaagccgc acacgaggat ggtgatgacc ggcagcacga agacgaggat cggcttccgg
     1921 gtggcggtgc ccaccgcgaa ggccgtggcg gtgaggaaga agagggtggg caacgacagg
     1981 aagagggccg gcttcaggta attcaggaga ttgaacggcc cgcggacctc ctcggccacc
     2041 tcgctcggca ccacgtggtt gaagaacatg ctgaacagca cgtggagggc cagcacgccg
     2101 cacaccgccg cgaaaacgcc ggcgaacttg ccgtacacgt attcggccgg cgtcagcgga
     2161 gtggcgtgca ggatggcgcc gacctgatgc tcctcgtcgt ggatgaccgc catgccggcc
     2221 gccacggcca cgaagaaggc gtacaggagc aggaccagga acgaggcgat ctgcgtgacg
     2281 gcaaattcgg aggtgatgaa ggccttggtg ccccccacct ggctgtcgcc ggaggcaatc
     2341 cgcaccgagc cggccgagag gccccagagc gagaaagcca gcagcacgag caggaaccag
     2401 aagatgggac ggcgcgccgc gtgccccagc tcttcgcgga agacgaggcc cagccggccc
     2461 aggttcacga agggctcccg gcggccagga gtccgatgtc acctctgggg gcctggtcgg
     2521 cctggtccgc ccgccgcatg aggaggagat aggcgtcctc cagtgtggcc gtcaccggtt
     2581 cggcgccgac gggcggcagg tcatccggca cgtagaggcg ggcccggttc tttccttcga
     2641 agaggatggc ctggagaacc gtatgccgcc tctcgagcgc ggccagttcc tcggcggtga
     2701 cggccacttc gaacacgcgt ccggtcattt ccgtccgcgc ggcgcccggc gtcgtgacgg
     2761 agaggagttg tccccccttg atcaccgcga atcgcgggca gaggaccgac acgtcttcca
     2821 cgatgtgcgt cgagaggagc acgatccgct gctcggccag ctcggccagc aggcggtaga
     2881 accggtgacg ctcctcagga tcgaggccgg cggtcggttc gtccacgatg atcagttccg
     2941 ggttgccggc gatggcctgg gcgattccga ggcgctgccg catgccgccc gaatatccct
     3001 ttaccttccg cttcgccgcg aacgtcaagt tcacgcggtc gaggagttcg gccacgagcc
     3061 tcttgaggcc acccggcgcc tcgaccccct tcagctcgag aaggtagcgg agcatctgct
     3121 cgccggtcag gtgcgggaag aagccgaact cctgggggag gtagccgagc cggttccaga
     3181 gccgttccgg cctggcgagg atgtcctccc cgccgagcgt caccgtgccg gatgtcggct
     3241 cgagcagccc ggccaggatc ttcatgaagg tggacttgcc ggcgccgtta gggccaagga
     3301 gcccgaacat ccccttgccg acgtcgaggt cgattccctg gagtgctgcg acgggaccgg
     3361 gatagatctt cacgagtcgg cgaacggaca gcatcggacc tccggggagc gggcctcgag
     3421 tttcgggttc agcccgccgg tgtacgtcgg atgcggaggt cgggttgccg gaagtcggcg
     3481 ggccggccgc gcagtatact ccgctcatgt ccgatccgaa ttccatcgag ccgggaccct
     3541 cggaaaccac cccggtcacg gtcgtcatcg gcccggcgcc cttcaacgcc cacgagggga
     3601 tcgccgagct gctccgatcg gcagcgcttc cggttcccga tcccgaagac agccccgtcc
     3661 agatgatggc cgccttcgcg ggccctcacc tgatcggctg catcggctgg gagcagtacg
     3721 gcgcgagcgc cctcgtccgc tcactggccg tgctggagga tgtccggggc gagggggtcg
     3781 gcaccgcgct ggttggtgca ctcgtggccg tcctcaaagg aatcggcgtc gacgaggcgt
     3841 ggctgctcac cacggacgcc gccggctggt tcgagcggca tgacttcgag cgcacggacc
     3901 gcgaggcaat tcccgaagcc gtccgggtgg cccgcgaaat ccgcctccct tgctgtgcca
     3961 ccgcccaggt gatgcggcgg cgcctcgact cctagcgctt cattcccggc acaccgccgt
     4021 ccatctcgag cgtgttcagg atcgtctgtg cttcccggtc ctccgggttg tgcaagagca
     4081 ccgcctcggc catccgccgc gactccgccg ggcggaagag cgcctggagc gagtaggccg
     4141 ccgacttcaa tgcccagtcc gatgtcggat tggtggcgag catggccgtg gcaacggcca
     4201 gcgactgcgg cgtctccatc cgccggaggc gctcatcacc ctccgctttc agcgagtcga
     4261 ccggcagcga gtcgctcaag gcaatcccga aggcccgatt gatggccgcg gcatgttcgc
     4321 cctggcggcc ggccaggtag ctggtggcgg ccacgccgag ccaggcggcc cggttcgccg
     4381 gatcgccctc tgccacggcg cggtaaagac gctccgcctc cggaagatct ccccgtcgga
     4441 tggcggcggc cccgcgccac agtcgcgttc gcggaagttc acgcgcggcc ggcgacatca
     4501 ggcggaagaa ctggtcggga tcccgcaggg cccccgactt cagtatccat tcgccaagca
     4561 gcaggcgatt gaagtcgacg cgggaataac catcggggac cggcaccccc ttcttgaacg
     4621 ggctcgattc ccgtttcacg tatggcaggg ggtcgaggac ggcgtacagc gtggctgcca
     4681 tcacgtcgcc gctggagcgg cgccatgtcc caccgccggc gagcgcgagc ccctggggga
     4741 tctcggcggc cgatttcggg atccagatgg aggccagcga cgggtacacc gccacttccg
     4801 agcccgacac gaggagatag tgcgcgccgc tcgaccgcaa ctgctcgatc gagccggccg
     4861 gtatgccgac cgtgggtgat cccgccatgt agggaaagtg gggcttccgc gccgcgatcg
     4921 gatgttcggg tcccagcggc acgtttcgcc tccgggcatc ggcaagcagc gtcacgtact
     4981 cgccgggcat cccaccgaat cgcttgggat cggtcgccag ccggaatccg gcgatcgaca
     5041 gcacgatcgt gagaacgccg aatgccccga gaagcggggg aaggagccgg ccgatgccgc
     5101 tcggccccgc cttcgcgccc ggtttcggcg cacgtccttt cccgcccgcg cgcggcgttc
     5161 ctgcagcggc cggacgatag ctccccagcc acacggccgt tccaccggcc atcgcgcccc
     5221 agtacgggag gagcggcagc atgaatcgct cgttgtagaa gaccgggaga aggctcaggt
     5281 aggtcaggac gccggtggca aggaacgcca gggcccggcg attcctccac gactgccagc
     5341 cgagcaggag gccccccacc gccacgatgg cccacggcac tccgacaagc ttcgtggcgt
     5401 catcgcgcag gtgatcgacg acattcgatg ccatggcctt gaggaacagg ccgggatcga
     5461 cgtcccacac gtccttgagg gacgcgatgt tggtggcgct catgaaaccg gtggtctggg
     5521 cgcggttggc gtcgtcgctc agcacctcga tcgcgatgtt ctcgtaagcc ttgttccaga
     5581 aaggagtgcc ggtcttcgcc atcacgaaga ggagccaggg gaggcaggtc acgatccagg
     5641 cgcccaccca ggctcctgcc gccgacagca tccgtcgcga cgccccgaag aggaggagcg
     5701 cggtcaggag tgccgcgggc aggaggaccg aaccgttgta gcgcgtcatc caggccaggc
     5761 ctcccagtgc gccggccact ccccagtcgc ggagtacgct tccttcgcct cgcaaccccg
     5821 cgtcgccccg cgacccggcg tcacgccgga agaggagatt caacgagccc atgacgagcg
     5881 cgaggaagag gaggtcgttg caggctcgat
//
LOCUS       DASVOD010000502         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_605968, whole genome shotgun sequence.
ACCESSION   DASVOD010000502 DASVOD010000000
VERSION     DASVOD010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605968"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..>2403)
                     /locus_tag="VF720_11240"
     CDS             complement(86..>2403)
                     /locus_tag="VF720_11240"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7879977.1"
                     /translation="DGGAWFAVASDLLEEELNLTGGRRVLYHVSAGGLITRRSDPATQ
                     PSREAEVIGISIDGALLAYSARDPNMGGQRTLRPWILTVTSGQVAAVGPPVGAEGIQG
                     VFSPLGQRVVMSSDADYLGTNGARSPQIFRADVDGATFQQLTFATNNGAIHPATNADG
                     TLVALESASPLLLGDPTGTPRVYLHVTGSPLKRIASGWQPALAADGSRVVYAASGDSV
                     GENGDGSFEIFSAKTDGTDKRQHTNYASAQAEDPAVSADGMRIVFAATSNLDGGNGDG
                     SREIWAIQSNGSGLRRLTDTAAPLGCREPAISPDGQWVAFASDADLVPNGNADGNEEI
                     FRIPFNGGDLLQVTDTSTGNNRRPRLSSDGRRLFFVSTANLLTGAQMGSGRLAFWRES
                     TGAFQLLSPASDRPIDALYVSHDGTRAAILSNASMEGRNPTHAIRIFAAATDGTLFRG
                     VTVAGGLTTQGLGFSGNGEWIAAGDASGQIVLIPWSGGTPDTVLSMAGIVGGFPSLNL
                     DGSRLSFVSLASGMGYRAGDVYRLERGADPAPEGLLEHTQPLPPTPPALAANGTTVVF
                     MMTGVDSLNPDGSREVFAARLPTTAVRWLDLSARLEGDDVVLAWRADTDGDHAAFRIQ
                     RAPTAEGPWDRRPGAPTSRDAQWEFREHDDPTARWYRIEAIDRFGRVDRSLPIRPADD
                     AARLRLTVGPNPTRGVSHIELVREVPGWNRVDIVSVTGRLVRRLVDESAGAGVYRLDW
                     AGDDASGNRVAPGVYYVRVDGMPARSIVRLR"
BASE COUNT          377 a          793 c          866 g          367 t
ORIGIN      
        1 gtggcggtaa tggccaccgc gagcagcgca gaaagtgcca gaatcgtcat cctcatcctc
       61 gtcatcctcc tcttgattgt cgcgttcagc gaagtcgaac gatcgaccgg gcgggcatcc
      121 cgtccacccg gacgtagtag acgccgggag cgacgcgatt gcccgatgca tcgtcgccgg
      181 cccagtcaag acgatagacg cccgcgccgg cactttcatc tacaagtcgg cgcaccagcc
      241 ggccggtcac cgaaacgatg tccacgcggt tccacccggg gacttcccgg accagctcga
      301 tgtggctcac gccacgggtg gggttgggcc ccacggtgag gcggagcctg gccgcgtcgt
      361 ccgcggggcg gatcgggagg gaacggtcca cgcggccgaa gcggtcgatg gcctcgattc
      421 gataccaccg agcggtcggg tcatcatgct cccggaattc ccactgcgca tcgcgggagg
      481 tgggtgcgcc ggggcggcga tcccaggggc cctcggccgt gggggcccgc tggatccgga
      541 aggcggcatg atccccgtcc gtatcggccc gccaggccag aacgacgtca tccccctcga
      601 gacgcgccga caggtcgagc cagcggaccg ccgtcgtggg gaggcgggcg gcgaatacct
      661 cgcggctccc gtccgggttg agcgagtcga cgccggtcat catgaagacc accgtggtgc
      721 cgttggcggc cagcgccggt ggtgtggggg ggaggggttg agtgtgctcg agcagcccct
      781 ccggcgcggg atcggcgccc cgttccaacc ggtagacgtc gccggcccgg tagcccatcc
      841 cgctggcgag cgaaacgaac gagaggcgcg acccgtcgag gttcagcgac ggaaaacctc
      901 cgacgatacc tgccatcgaa agcaccgtgt ccggcgtccc gccggaccag gggatgagga
      961 cgatctgtcc actcgcgtcg ccggcggcga tccattcacc gttgcctgaa aagccgagcc
     1021 cctgcgtcgt gaggccaccc gccacggtca ctccccggaa gagggtgccg tcggtggcgg
     1081 ccgcgaaaat ccggatggcg tgggtggggt tgcgcccctc catggaggca ttcgagagga
     1141 tggccgcccg cgtgccgtcg tggctcacgt acagggcgtc gatgggtcga tcggatgcgg
     1201 gcgagaggag ttgaaaagcg ccggtggatt cccgccagaa ggccagtcgg ccgcttccca
     1261 tctgagcacc cgtcaggaga ttggccgtgg agacgaaaaa caggcgcctg ccgtcgctgc
     1321 tgagccgcgg gcgccggttg ttgccggtgg acgtgtcggt gacctgcagc aggtctcctc
     1381 cgttgaaagg gatccggaag atctcctcgt tgccatcggc gttgccgttc gggacgaggt
     1441 cggcgtcgct cgcgaaggcc acccactggc cgtcggggga gatggccggt tcacgacatc
     1501 cgagcggggc ggccgtgtcg gtcagccggc gcagtccgga gccgttggac tgaatggccc
     1561 agatctcccg gctcccgtcg ccgttgccac cgtcgaggtt gctggtggcg gcaaagacga
     1621 tccgcatacc gtcggccgag acggccgggt cctccgcctg ggcggacgcg tagttcgtgt
     1681 gctggcgctt gtcggtgccg tccgttttcg ccgagaagat ctcgaacgag ccgtcgccgt
     1741 tttcgccgac cgaatcgccg gaggcggcgt acacgacgcg cgaaccgtcg gccgccaggg
     1801 ccggttgcca tcccgaggcg atccgcttga ggggtgatcc ggtcacgtga aggtagacac
     1861 gcggcgtgcc ggtcgggtcg ccgagcagca ggggcgacgc cgattcaagt gcgacgagcg
     1921 tgccatcggc attcgtggcg gggtggatcg caccgttgtt ggtggcgaag gtcaactgtt
     1981 ggaacgtggc gccatccaca tcggcccgga agatctgcgg cgagcgcgca ccgttcgttc
     2041 ccaggtagtc ggcgtcggaa ctcatcacga cccgctgtcc gagcggcgag aagaccccct
     2101 ggatcccctc ggcaccgacg ggggggccga cggccgccac ctggccgctg gtcaccgtga
     2161 gaatccacgg ccggagcgtg cgctggccac ccatgttcgg atcgcgggcc gaataggcca
     2221 gcagcgcgcc atcgatcgag atgccgatca cctcggcttc gcgcgagggt tgggtggccg
     2281 gatccgaacg ccgcgtgatc aggccgccgg ccgacacgtg gtagaggaca cgccgtcccc
     2341 cggtgaggtt gagttcctcc tcgaggaggt cgctcgccac cgcgaaccag gcgccgccgt
     2401 ccg
//
LOCUS       DASVOD010000503         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_189821, whole genome shotgun sequence.
ACCESSION   DASVOD010000503 DASVOD010000000
VERSION     DASVOD010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189821"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="VF720_11245"
     CDS             complement(<1..444)
                     /locus_tag="VF720_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEX7879978.1"
                     /translation="MTDLKTLAVEKRPDRVRLPGRLLFLTEDPDLLTGQLAGQDLAWN
                     PSIKLRDDISTDEITPAYICYYFDETLGDFPYLGLKAGGEFPVKRGLVRAGGFSVAVS
                     GKRRGKGSSREQSPYAELCAGIQCVVAENIERIYRQNCQNLGVLTS"
     gene            675..3383
                     /locus_tag="VF720_11250"
     CDS             675..3383
                     /locus_tag="VF720_11250"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HEX7879979.1"
                     /translation="MTPLSSDLSAVIDSYLGSKGESAPDLTHLLEFREAVRAAIAAGE
                     GGALLMHLKSLTSGTGLHVGRVYLEGLLAAGRNDVATADLSFQPLLEKLNQAQVWPVL
                     GRAALDWIEGAGTHGGVRYLVRAWKQGGVAAVDESMLRIANEVLPTEPDIIWALATTL
                     DEKGSSKESRRLLATALKPLAEHKDAARVEEGILRLIEAPEASILLHALEAVDTLSHK
                     GDGKSAAALLESLRDTSISHGLAGPGWAIARRGMEKHPNETGFRPSAVAFLKAAHPEV
                     PSLDRVIDVTGLDRVAVPAAQALKALDIVLPFSPGYYVEHSGWGLGPIRDNDGEFLTI
                     DFPTKPGHRMKLDSAAKVLTSLPADDLKVLLAIDKPRLLTMRDDDPAALIVIALKRAG
                     GKGSATELRKLLVPGVLATGEWASWWKKIKPRLVEDARIDSRQSFSDNFRLPVPGEET
                     DEPFVLPPFDEPKGINHNVDLIIDFLDHHPAQEEQLKAQTLDRVQRWADRRAAKRHER
                     ARAGLLLLKWDPAGRAKHEAALREHFANDFDLSAAGGSDEQLALLDIGISSPETRESA
                     LRLGLNARSSIVRDAALKTLLAEPGPGPIKFVADLYQDPVEYTDALFMGADAVASREG
                     KGTADPRLDDLLWPVMLGLIDLLNVTGREPIRKKGLKYLEAGSSLMARVTELPPNERV
                     EAQLTARLRDWRQSDRILFPILDTFSEAGLTTIVESVRQKRKAAHEKLFAGAPAADDV
                     TAGPVIMSRATFDILHAELQQINLDLKTVIPQAIKKARELGDLRENAEYEAAKLKQAN
                     ASKRLSQLDAQLGKVRILDDMTIDPTKGGPGTEVTVRDTADGTVTTYWVLGEGDGHLG
                     PNVISYLAPLGRALSGRAVGDMVDYQPTDGGGMKKLEITGITIRKP"
     gene            complement(3477..3947)
                     /locus_tag="VF720_11255"
     CDS             complement(3477..3947)
                     /locus_tag="VF720_11255"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7879980.1"
                     /translation="MALSQVSLTQEDGVVCQIFTKGHIITADDPADPKGEDEGPTPLE
                     YLLAALGSSAAIALRRAGDARDLPIEEVQVQTKYRATFASLAAGTDDPVSAGAIQREL
                     RVRITRDITEDERDALLAAIKSSPVDRTLGTGVKIEDALYILGYAQPDEGVIVE"
     gene            4059..>4707
                     /locus_tag="VF720_11260"
     CDS             4059..>4707
                     /locus_tag="VF720_11260"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEX7879981.1"
                     /translation="MSIARLLIVGNEVLSGEVTDRNIAFLTRRLTELGTRVTGVRIVP
                     DEDDAIVEGVREAIVPGHPLLVTGGIGPTHDDRTRPAVARALELPLARHAEAEERLRA
                     SYGARLTGAELEMAMLPAGARLAAGPASGVCGFVVGRVYVFPGVPDLLVDVFEAVAAD
                     FVGAPLHRVEVATPIKEGDFAPALAALAEEYPDVAIGSYPVRNGAGWMVRLILRGT"
BASE COUNT          776 a         1590 c         1607 g          734 t
ORIGIN      
        1 ggaggtgagg acgcccaggt tctggcagtt ctgccggtag atccgctcga tgttttcggc
       61 caccacgcac tggatgccgg cgcacagctc ggcgtagggg ctctgctcgc ggctcgaacc
      121 cttgccgcgt cgcttgccgg aaactgccac cgagaagccg ccggcacgga cgagaccgcg
      181 ctttaccgga aattcaccgc ccgccttgag gccgaggtag gggaagtcgc cgagcgtctc
      241 gtcgaagtaa tagcagatgt aggcgggggt gatctcatcc gtcgagatgt cgtcccggag
      301 cttgatcgat ggattccacg ccaggtcctg cccggccaac tggccggtca ggagatcggg
      361 atcctcggtc agaaagagga ggcgaccggg gagccggacg cgatcgggac gtttttccac
      421 ggcaagggtc ttgaggtcgg tcatcgaggc ctcggaagtt cgacgaagtc agaagcggcg
      481 gcgccgcagg gcgcccgacc gcgattcgag cgatcgagag tgttagagta tcacatgaag
      541 ggtgttgatc ggttgcctca caaccccttt ctgcgttata acttgtcgcc tttccttcgc
      601 gcacgatccc ggggcccgtg ccgcccttcg ccggcggccc cattgccccg ctgaccatga
      661 tgggagaacc accggtgacg ccactctcca gcgacctctc ggccgtgatc gacagctacc
      721 tcgggagcaa gggagagtcg gcgcccgacc tcacccacct cctcgaattt cgcgaggcgg
      781 tgcgcgcggc catcgccgcg ggtgaagggg gtgcgctgct catgcacctg aagtcgctga
      841 catcgggaac ggggctgcac gtcggccggg tgtacctcga agggttgctc gcggccgggc
      901 ggaacgacgt ggccaccgcc gacctctcct tccagccgct cctcgagaag ctgaaccagg
      961 cgcaggtgtg gccggtgctc gggcgcgcgg cgctcgactg gatcgaagga gccggcacgc
     1021 acggtggcgt gcgctacctg gtccgcgcct ggaagcaggg gggcgtggcg gcggtcgatg
     1081 agtcgatgct ccggatcgcc aacgaggtgc tccccaccga gcccgacatc atctgggccc
     1141 tggccaccac gctcgacgaa aagggttcga gcaaggagag ccggcgcctc ctggccacgg
     1201 cgttgaagcc cctggccgag cacaaggacg cggcccgcgt ggaggaagga atcctccggc
     1261 tcatcgaagc gcccgaggcc tccatcctgc tgcacgccct cgaggcggtc gacaccctct
     1321 cccacaaggg agatggcaag tcggcggccg ccctgctgga aagcctccgc gacacgtcca
     1381 tcagccacgg cctggcgggc ccggggtggg ccatcgcgcg gcgcggcatg gagaagcatc
     1441 ccaacgaaac cggcttccgc ccgtcggcgg tggcgttcct caaggcggcg cacccggagg
     1501 tgccgtcgct cgaccgggtc atcgacgtga cgggactcga ccgcgtggcg gtgccggcgg
     1561 cccaggcatt gaaggcgctc gacatcgtgc tcccgttctc tccgggctat tacgtggagc
     1621 actccgggtg gggcctgggc cccatccggg acaatgacgg agagttcctc accatcgact
     1681 tccccaccaa gcccggccac cggatgaagc tcgactcggc ggccaaggtg ctcacgagcc
     1741 tcccggccga cgatctcaag gtgctcctgg ccatcgacaa gccgcgcctc ctcaccatgc
     1801 gcgacgacga cccggccgcc ctcatcgtca ttgcgttgaa gcgggccggc ggcaaaggct
     1861 cggccacgga gctgcgcaag ctcctcgtgc cgggcgtgct ggctaccggc gagtgggcct
     1921 cctggtggaa gaagatcaaa ccgcgcctcg tggaagacgc ccgcatcgac agccggcagt
     1981 cgttcagcga caacttccgc ctcccggtcc ccggcgagga aaccgacgag ccgttcgtcc
     2041 tccccccctt cgacgagccg aaggggatca accacaacgt ggacctgatc atcgacttcc
     2101 tcgaccatca cccggcccag gaagagcagc tcaaggcgca gacactcgat cgcgtgcagc
     2161 gctgggcgga ccgtcgcgcc gccaagcgcc acgaacgggc ccgggccgga ctgcttctcc
     2221 tgaagtggga tcccgccggt cgcgcgaaac acgaggccgc gctgcgcgag cactttgcca
     2281 acgacttcga tctctccgcc gccggcggat cggacgagca gctggcgctg ctcgacatcg
     2341 ggatttcatc gcccgaaaca cgcgagagcg cccttcgact ggggctgaac gcccgctcgt
     2401 ccatcgtccg ggatgcggcc ttgaagacgt tgctggccga gccgggcccg gggccgatca
     2461 agttcgtggc cgacctgtac caggacccgg tggagtacac ggacgcgctc ttcatggggg
     2521 ccgatgccgt ggcttcccgc gaggggaagg gcaccgcgga cccgcggctg gacgacctcc
     2581 tctggccggt gatgctcggc ctgatcgatc tcctcaacgt gaccggccgc gagccgatcc
     2641 gcaagaaggg gctcaagtac ctcgaagccg gttcgagcct gatggcgcgc gtcaccgagc
     2701 tgccgcccaa cgagcgggtg gaggcccagc tcaccgcgcg cctgcgcgac tggcgccagt
     2761 ccgatcgaat cctcttcccg attctggata cgttcagcga ggccggcctc accaccatcg
     2821 tggaatcggt ccgccagaag cggaaggcgg cccacgaaaa actctttgcc ggtgcgccgg
     2881 ccgccgacga cgtgacggcc ggaccggtga tcatgtcgcg cgccacgttc gacatccttc
     2941 acgccgagct gcagcagatc aacctcgacc tcaagaccgt catcccccag gccatcaaga
     3001 aagcccgcga actgggcgac ctccgggaga acgccgagta cgaggccgcc aagctcaagc
     3061 aggccaacgc cagcaagcgc ctctcccagc tcgacgccca gctcggcaag gtccgcatcc
     3121 tcgacgacat gacgatcgac ccgaccaagg gtggcccggg taccgaagtg accgtgcgcg
     3181 acaccgccga cggtacggtg accacctact gggtactcgg cgaaggtgat ggccaccttg
     3241 gcccgaacgt gatctcgtac ctggcgccgt tgggccgggc gctttcggga cgcgcggtcg
     3301 gcgacatggt cgactaccag ccgaccgacg gcggcgggat gaagaagctg gagatcacgg
     3361 ggattacgat aaggaagccg taggccgacc tgttttggtg gctgcggctc ggtggtgccg
     3421 gtggaaccga cgtcgtggag ccgcctgaag gcggtcctgc ccctcggccg gtagggctac
     3481 tcgacgatca ctccctcatc cggctgcgca taacccagga tgtagagcgc atcctcgatt
     3541 ttcacgcccg taccgagtgt gcggtcgacc gggctcgact tgatggcggc gaggagggcg
     3601 tcgcgctcgt cctcggtgat gtcgcgggtg atgcggacgc ggagctcgcg ctggatggcg
     3661 ccggccgata cggggtcatc ggtgccggct gccaggctgg cgaacgtggc gcgatacttc
     3721 gtctgcacct gcacttcctc gatcgggaga tccctcgcgt cgccggcgcg ccgcagcgcg
     3781 atggccgccg acgagcccag ggcggccagc agatattcga ggggcgtcgg cccctcgtcc
     3841 tcacctttcg ggtcggcggg gtcgtcggcg gtgatgatgt gccccttggt gaagatctgg
     3901 cagacaacgc cgtcttcctg ggtgagtgaa acctgcgaca gcgccatcgg gcctccgggg
     3961 agatggttca gcggcggccg ccaccgggcg gccactcggt cgtcggacga cgccgacgac
     4021 accctatata atgtggcgat ccacggaggc agttcaacgt gtccatcgcc cgcctgttga
     4081 tcgtcggcaa cgaggtgttg tccggcgaag tgactgatcg aaacattgcg tttctcacgc
     4141 gccggctgac ggagctcggc acgcgggtta cgggcgtgcg catcgtgccc gacgaggatg
     4201 acgcaatcgt cgaaggcgtc cgcgaggcga tcgtgccggg gcatcccctt ctcgtgacgg
     4261 ggggcatcgg cccgacgcat gacgaccgga cgcgccccgc ggtggcgcgc gcactcgaat
     4321 taccgttggc ccgccatgcg gaggccgaag aaaggctgcg cgcgagttac ggcgcgcggc
     4381 tcaccggggc cgaactcgag atggcgatgc tgccggccgg cgcgcggctg gccgcgggtc
     4441 cggcttcggg cgtttgtgga ttcgtcgtcg gccgggtgta cgttttcccg ggcgtaccgg
     4501 atctcctggt cgatgtcttc gaggccgtgg ccgccgactt cgtcggcgcc ccgctccacc
     4561 gggtggaggt ggccacgccg atcaaggaag gagactttgc tccggcgctc gcggcgctgg
     4621 ccgaggaata tcccgacgtg gcaatcggct cttaccccgt tcgcaacggc gcgggatgga
     4681 tggtgcgcct catcctgcgc gggacgg
//
LOCUS       DASVOD010000504         1908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_138505, whole genome shotgun sequence.
ACCESSION   DASVOD010000504 DASVOD010000000
VERSION     DASVOD010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138505"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF720_11265"
     CDS             <1..376
                     /locus_tag="VF720_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX7879982.1"
                     /translation="WGGTTPKGFDCSGLTQLVHDLHGLPLPRDSKDQDAWLARRSAAI
                     PDPLAVPPGGLLFFGAPGGAVSHVGFSLGGGAFLHAQGRVRQHTLDPSNPQYHKDLAA
                     GFRRGFAPHPGMKPPRQSPPRG"
     gene            422..1663
                     /locus_tag="VF720_11270"
     CDS             422..1663
                     /locus_tag="VF720_11270"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX7879983.1"
                     /translation="MYYFFYIPIGTELSVRKPPAAIITLTTLNIVWFLLFSCLPPLVP
                     LRPAMGFDASHPLLVNAVTACFIHADWFHLLGNLVFLVTLGPALEDRIGPGKFLGLYL
                     LSGMAGMAVQAEAWRQGAFGATEPLIYGASGAIAGVLGAFVLRCGFARVQVAHVTLAL
                     VQGQARAGTTPINGYLAVASWGFLQVVYAMVAQVVGGTSVAYAAHMGGLGAGLLLGVG
                     LGLPAEAAIENLWLKSRRHAQRGDHFAALGELLTFITYRPKDAAAWLEAARLQRILKR
                     HRDATRSWFRGIVILWIERRRAEAVVAARELRRHYPGARLRPSVLFRVGLFLERQGDL
                     GWASHTYEDYGRFYPQHERAPRALLRAARIESRSRNDLERARRLYETLGTRYPNAPET
                     AIGLRELAAIDRVLARRQEAA"
BASE COUNT          266 a          668 c          629 g          345 t
ORIGIN      
        1 gtgggggggg acgacgccca aggggttcga ctgttccggg ctcacccagc tcgttcacga
       61 tctccatggc ctgccgctcc cgcgcgattc gaaggaccag gatgcctggc tggcgcgacg
      121 atcggcggcc atcccggacc ccctggccgt gccgccgggc gggctcctct tcttcggcgc
      181 cccgggaggc gccgtctcgc atgtggggtt ttcgctggga gggggggcgt tcctccatgc
      241 ccaggggcgc gtgcggcagc acacgctcga tccgtccaat ccccagtatc acaaggacct
      301 ggcggccggt ttccgccggg gcttcgcgcc ccaccccgga atgaagccgc cccgtcaatc
      361 gccgccccgg ggatgaccgg ctttcatcaa gtctccgcgc cccgcgggcg ataactgacg
      421 ggtgtactac ttcttctaca tcccgatcgg caccgaactc agcgtcagga aaccccccgc
      481 ggcgatcatc acgctgacga cgctgaacat cgtctggttc ctcctcttca gttgcctgcc
      541 gcccctcgtt ccgctccggc cggcgatggg cttcgatgcc tcgcatcccc tcctcgtcaa
      601 tgccgtcacc gcctgcttca tccatgccga ctggtttcac ctgctcggca accttgtctt
      661 cctggtcacc cttggtccgg cgctcgagga tcggatcggc ccgggaaaat ttctcggcct
      721 ctatctgctg tccggcatgg cggggatggc cgtccaggcc gaagcgtggc gacagggtgc
      781 gttcggcgcc accgaaccgt tgatctatgg cgcatcgggg gcgattgccg gtgtgctcgg
      841 cgcgttcgtg ctgcgctgcg gatttgcgcg cgtgcaggtg gcccacgtga ccctcgccct
      901 ggtgcagggg caggcccgcg ccggaacgac cccgatcaat ggctacctgg ccgtggcgtc
      961 gtggggtttt ctccaggtgg tgtacgccat ggtggcgcag gtcgtgggtg gaacgtccgt
     1021 ggcctacgcg gcgcacatgg gcgggctggg cgccgggctc cttctcggcg tggggctcgg
     1081 cctgccggcg gaagcggcga tcgagaatct ctggctcaag tcgcggcgcc atgcgcagcg
     1141 tggcgatcac ttcgccgcac tgggtgagct gctgacgttc atcacgtatc gacccaagga
     1201 tgcggcggcc tggctcgagg ccgcacgcct ccagcggatc ctcaagcggc atcgcgacgc
     1261 cacccgttcc tggttccgcg gcatcgtcat tctctggatc gagcgacggc gcgccgaggc
     1321 ggtggtggcc gcgcgggagc tgcgacgtca ctaccccggg gccaggctgc gtccctcggt
     1381 gctcttccgg gtcggcctct tcctcgaacg gcagggggac ctcggctggg ccagtcatac
     1441 gtatgaagac tacggtcgct tctatccgca gcatgagcgg gcgccgcggg cactcctccg
     1501 cgcggcccgc atcgaatcgc gcagccgcaa cgatctcgaa cgcgcgcgcc gtctctacga
     1561 aaccctcggc acccgctatc cgaacgcgcc cgagacggcc atcggcctgc gtgagctggc
     1621 cgcgatcgac cgggtcctgg cccgacggca ggaggccgcc tgagccggga ggaaaaaccg
     1681 cacaactgac tgtaggatgg tcgtttggga cttgtcgccc attggctcct ctgatagcgt
     1741 accggcccgt cccaactcgc tgcacccaac acgattcatg tgagcgtcaa cgacactcga
     1801 tccacgccgg gcgggcggag gccgtgtccg cctgccccgc gttcataccg atcccggaaa
     1861 ggatggtgcc tggatgttcg accgactgat gggcatgttc tcgaacga
//
LOCUS       DASVOD010000505         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_451426, whole genome shotgun sequence.
ACCESSION   DASVOD010000505 DASVOD010000000
VERSION     DASVOD010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451426"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF720_11275"
     CDS             <1..402
                     /locus_tag="VF720_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding domain-containing protein"
                     /protein_id="HEX7879984.1"
                     /translation="GLNGFVGEFLVILGAFQTARPFAIVAALGVILSAVYMLWMFQRV
                     MFGEVTKEENRKLVDLSPREWAVLTPVLILIIWIGVHPKPFLKTSEVAVADILKRYGA
                     SQFGQVVTPPVHDISIPGADPSVPPRMEAAR"
     gene            399..1874
                     /locus_tag="VF720_11280"
     CDS             399..1874
                     /locus_tag="VF720_11280"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX7879985.1"
                     /translation="MSELVRIFGSLGINFSTIGPEIALLTTACFILLLDCFYKRNGGG
                     HLLGIGLVGLGVAAWLAWRVDPNFTYTAYYDNIGADPFSRIVKVILCGITAVTFLMSA
                     GYAQRREVESAEYYALMLLATLGMMLMAAGTDLIMIFLGLEISSISQYILAGFRHNER
                     RSVEASLKYFILGAFATGFLLYGIALVYGATGTTNLAQLKIYTTNVEKAPLLLMAGCA
                     LLLVGFGFKVSSVPFHMWTPDVYDGAPTTVTAFMAAASKVAGFAALTRILLVGFSGVH
                     SGWGQIVWVIAALTMTLGNIAALTQSRVKRMLAYSAIAHAGYLLVAIVPGTEIAGIGI
                     IYYLVAYALTNLGAFAIVGLLKTEDEEGLKLSNFAGLGYRRPWLGLLMALFMFSLAGI
                     PPTAGFLGKFYIFSGAIEAGYIGLAVLGVVNSVISVYYYLRVVVWMYMTPDVTGEDPL
                     ITASWPVRVSLALTAIGVLVYGLWPTPLVEMARKAILPLLG"
     gene            1927..>2479
                     /locus_tag="VF720_11285"
     CDS             1927..>2479
                     /locus_tag="VF720_11285"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7879986.1"
                     /translation="MRRLQLSFLILAALLPPPSIGQAQDAPPAPKLFAVEFTTGPAWD
                     HAEPPNEQRHFAEHSKNLRRLRDEGKLPMGARYGDKGLILVVAPDLEAAQAEFAEDPA
                     VTDSVFVHVIHEFSVFYPGRVERPSPKKDPDPPPPTVELPTDVRRVLTDYETHWRAKD
                     FAALAALFAGDGFVMASGGSPVRG"
BASE COUNT          382 a          873 c          752 g          472 t
ORIGIN      
        1 ggcctcaacg gcttcgtggg cgagttcctg gtgatcctgg gcgccttcca gaccgcccgc
       61 ccgttcgcca tcgtggcggc gctgggtgtc atcctctcgg ccgtctacat gctgtggatg
      121 ttccagcgcg tgatgttcgg cgaggtcacg aaggaggaaa acaggaaact ggtggacctc
      181 tctccccgcg agtgggccgt gctgacgccg gtgctcatcc tgatcatctg gatcggcgtg
      241 cacccgaagc cgttcctcaa gacgagcgaa gtggcggtgg ccgacatcct gaagcgctac
      301 ggcgcctcgc agttcgggca ggtggtgacc ccgcccgtcc atgacatttc gatcccgggc
      361 gccgacccga gcgtgccgcc ccgcatggag gccgcgcgat gagcgagctc gtccgcatct
      421 tcggttcgct cggcatcaac ttctcgacga tcgggccgga gatcgcgctg ctcaccacgg
      481 cctgcttcat cctgctgctc gactgcttct acaagcggaa cggcggcgga cacctgctgg
      541 gcatcggcct ggttgggctc ggcgtggccg cctggctggc ctggcgagtc gatcccaact
      601 tcacctacac ggcctactac gacaacatcg gcgccgatcc gttcagccgc atcgtcaagg
      661 tgatcctgtg cggcatcacc gccgtcacct tcctgatgtc ggccggctat gcgcagcggc
      721 gtgaggtgga gagcgccgag tactacgcgc tcatgctcct ggccacgctg ggcatgatgc
      781 tgatggccgc gggcaccgac ctcatcatga tcttcctcgg cctcgagatc tcgtcgatca
      841 gccagtacat cctggccggt ttccggcaca atgagcgccg cagcgtggag gccagcctca
      901 agtatttcat tctcggggcc ttcgccaccg gcttcctgct ctatggcatc gctctcgtgt
      961 acggcgccac cggcacgacc aacctggccc agctcaagat ctacaccacc aacgtggaga
     1021 aggcgccgct cctcctcatg gccggctgcg cgctcctcct ggtgggcttc ggcttcaagg
     1081 tgtcgagcgt tccgttccac atgtggacgc ccgacgtgta cgacggcgcc ccgaccaccg
     1141 tcaccgcctt catggccgcc gcctccaagg tggccggctt tgccgccctc acgcgaatcc
     1201 tgctcgtggg cttcagcggc gtgcactcgg gctggggaca gatcgtctgg gtgatcgccg
     1261 cgctcaccat gacgctcggc aacatcgccg cgctcaccca gagccgcgtg aaacggatgc
     1321 tggcgtactc ggccattgcc cacgccggct acctgctggt agccatcgtc ccaggaaccg
     1381 agatcgcggg catcggcatc atctattacc tggtggcata cgccctcacc aacctcggcg
     1441 ccttcgccat cgtgggcctc ctgaagaccg aagacgagga aggcctcaag ctctccaact
     1501 tcgcgggcct cggctaccgc cgaccctggc tgggcctgct catggccctc ttcatgttct
     1561 cgctggccgg catcccgccc accgcgggct tcctcggcaa gttctacatc ttcagcggcg
     1621 ccatcgaagc cggctacatc ggactggccg tcctcggtgt cgtgaactcc gtcatctccg
     1681 tctactacta cctgcgcgtc gtcgtctgga tgtacatgac tcccgacgtg accggcgagg
     1741 acccgctcat cacggcctcc tggccggtcc gcgtctcgct ggcgctgacc gccatcggtg
     1801 tgctggtcta cgggttgtgg ccgacgcccc tggtggagat ggcgcggaag gcgatcctgc
     1861 ctctcctggg gtagccggtc cgccacgcat gctacgatcc cgcctctcat ttcacgaggt
     1921 ggatcgatgc gtcgtttaca actctccttt ttgatcctcg cggcactgct gccgcccccc
     1981 tctatcggcc aggctcagga cgctcctccc gccccgaagc tatttgccgt cgaattcacg
     2041 accggaccgg cctgggatca cgccgaacct ccgaacgaac agaggcattt cgcggagcac
     2101 tcgaaaaacc tgcgccggct gcgggacgaa ggaaagctcc cgatgggggc gcgctacggc
     2161 gacaaaggat tgattctcgt cgtggcgccc gatctcgagg cggcgcaggc ggagttcgcc
     2221 gaggatcccg cggtcaccga cagtgtcttc gtgcatgtca tccacgaatt ctccgtcttc
     2281 taccccgggc gggtggaacg accgtcaccg aagaaggacc cggacccgcc gccaccaact
     2341 gtcgaactgc cgaccgatgt ccggcgcgtc ctgaccgact acgaaacgca ctggcgggcg
     2401 aaggatttcg cggctctggc cgccctcttc gcgggagatg gtttcgtgat ggccagcggc
     2461 gggtcgccgg tacgtggcc
//
LOCUS       DASVOD010000506         1925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1027538, whole genome shotgun sequence.
ACCESSION   DASVOD010000506 DASVOD010000000
VERSION     DASVOD010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027538"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1016
                     /gene="trpC"
                     /locus_tag="VF720_11290"
     CDS             237..1016
                     /gene="trpC"
                     /locus_tag="VF720_11290"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX7879987.1"
                     /translation="MILEQIVRRKKDEISCLMASDPARLPRADRDFGQALGRGRSEVR
                     LIAEVKRASPSAGLLTSDFDPVEIAHAYQGGGAAAVSVLTDRDFFGGSIDDLRRVSEG
                     VSLPVLRKDFVIDERQIHEARDAGAHAILLIAAILDRKTMQRFLAESRALGMEPLVEI
                     HDERERDVALEAGASIVGINNRNLDDFRIDTGTTFRLAAGLPHGLTIVSESGVESESG
                     VAALVGHADAVLVGTALMRSADRKGFAAALVKAGRNRADEQ"
     gene            1270..>1925
                     /locus_tag="VF720_11295"
     CDS             1270..>1925
                     /locus_tag="VF720_11295"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX7879988.1"
                     /translation="MPDSFTTSAPPTVSAGDFRRALLGWYRANGRPLPWRLDPSPWRV
                     WISEMMLQQTTVATVLPRYESFLERFPTVTAMARAKVEDVLAAWSGLGYYTRARNLHA
                     AAVRVVREHQGVFPKEPAVVRDLPGFGDYSTAAILSIVHGVPLAVVDGNVIRVVSRLA
                     MLPGHAKSPALRKAVQIEADRLIDVEEPGDFNQAMMELGARVCTPTSPDCDGCPVEAF
                     "
BASE COUNT          336 a          623 c          630 g          336 t
ORIGIN      
        1 aatcaagcgc ggctcaacgc ttgcggccaa cccgttacgc cccgaggcaa actctattct
       61 catttcagcc aagtgcaagt gataaatatt gtcctgacga ggccggaggg cccacccggc
      121 cacccgggcg tcgccacgcc cgacggtcgg agcgccggtt cggggccaga ctcgatgcta
      181 caacttgtta gtgcataatc acttgaagcc aagccacccg gggacaaggg aggaccatga
      241 tcctggagca gatcgtacgg cggaagaaag acgaaatttc atgcctgatg gcatcggacc
      301 cggcccggct ccctcgggcc gaccgggatt ttggccaggc gctcgggcgg gggcggtcgg
      361 aagtccgttt gatcgcggag gtgaagcggg cttcaccgag tgcaggcctc ctcacttccg
      421 actttgatcc ggtggaaatc gcgcacgcgt accagggcgg aggggccgcc gccgtgtcgg
      481 tgctgaccga tcgcgatttc ttcggcggct cgatcgacga cctgcggcgc gtgtccgaag
      541 gtgtctccct gcccgtcctg cggaaggatt tcgtgatcga cgagcgacag atccacgagg
      601 cgcgcgatgc cggcgcccac gcgatcctcc tgatcgccgc cattctcgac aggaaaacga
      661 tgcagcgatt cctggccgaa tcccgcgcac tggggatgga accgctggtc gaaatccacg
      721 acgaacggga gcgcgacgtg gcgctcgagg cgggcgcctc gatcgtcggc atcaacaacc
      781 gcaatctcga cgacttccgg attgacacgg gaaccacctt ccggctggct gccggacttc
      841 cccacggact cacgatcgtg agcgagtcgg gcgtggagag cgagtcgggg gtggccgcgc
      901 tcgtcggtca tgccgacgcg gtgctggtcg gaacggcgct catgagatcg gccgaccgga
      961 aggggtttgc cgcggcgctc gtgaaggcgg gacggaaccg cgccgacgag caataaaaaa
     1021 cccgcgagcg ttgtcggcat tcgccgcctc gactgaccag gtcgaagact cgcgggtctg
     1081 gtgccgggtt ggaacttagc cgggatccgg cctcgaccaa ccagcatcgg agtcggttcc
     1141 tagctaacca ggcacctcgc aggtctattg attttactca aaggaccacc tcctttcttg
     1201 cgtgccgaaa cgctagcatc cgggagtggc gggccacaag ggaaatctcg ctgcgttaca
     1261 cttctcccga tgcccgattc gttcaccacc tccgcgcccc cgaccgtttc cgccggcgac
     1321 ttccggcgcg cgctcctcgg ctggtatcgc gccaacggtc gtccgctccc gtggcggctc
     1381 gatccctcgc catggcgggt gtggatctcc gagatgatgc tgcagcagac gaccgtggcc
     1441 accgtccttc cccgttacga gagttttctc gaacgttttc ccaccgtgac cgccatggcc
     1501 cgggcgaaag tggaggatgt gctcgccgcg tggagcggcc tcgggtatta cacccgcgcg
     1561 cgcaaccttc acgcggcggc cgtgcgggtc gtccgcgagc accagggcgt cttcccgaag
     1621 gaacccgcgg tcgtccgcga cctccccgga ttcggcgact attcgacggc cgccatcctg
     1681 tcgatcgtcc acggtgtgcc gctggcggtg gtcgatggca acgtgatccg agtcgtttcg
     1741 cggctggcca tgctgccggg gcatgcgaaa tccccggcgc tcaggaaggc ggtgcagatc
     1801 gaggccgacc ggctgatcga tgtcgaagaa ccgggcgact tcaaccaggc gatgatggaa
     1861 ctgggggcaa gagtgtgtac gcccacctcg cctgattgcg atggatgccc ggtcgaggcg
     1921 ttctg
//
LOCUS       DASVOD010000507         5942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_398027, whole genome shotgun sequence.
ACCESSION   DASVOD010000507 DASVOD010000000
VERSION     DASVOD010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398027"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..3344
                     /locus_tag="VF720_11300"
     CDS             162..3344
                     /locus_tag="VF720_11300"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX7879989.1"
                     /translation="MIENQGDMAPRENEPGLSLEAIRTRLAGKTGKVYWRSLEELADA
                     PEFTDLLHREFPRQASEWNEGVSRRDFLHVMAASLALAGLSGCAPRNPAEKILPYVKA
                     PEGFIPGKPLYYATSIMDGGAATGVLVESHMGRPIKIEGNDRHPASLGATSAPLQASI
                     LTMYDPDRAQVVTNLEEVRPWTSFVTAMTASLAAQKITGGSGLRILTETINSPTAAAQ
                     LEKILADYPAARWHQFEPLNRDNVVEGARLAFGEPVDTHFNLLEARVIVSLDADFLFS
                     GPGHLRYVRDFVRGRRVRTPGSGHGADSHGSATGVAPGSDPHQGQVEKSPTEYGAGPA
                     VAEHGESPTGAGRAAEPPKMSMNRLYVAESGFTNTGSRADHRLAVRPEVIESIAREIA
                     RALGIGAAPGGAPLDAKAERWVAGLVSDLRKNPGASAVMVGEQQPPAVHALAHAINAA
                     LGNVGGPVLVTAPIAARAENQLASIKMLADDMEAGRVQLLLILGGNPVYNAPPELDFA
                     RRLGKVGVRVHHGLYEDETSELCHWHIPDTHPLEAWSDGRAYDGTVSIVQPLIAPLYE
                     GKSIHEVLAVLTGETAKSGYDLVRDHWKSVMPGASFESTWSTAVHDGLLAGSAAAPFA
                     GAIRDAAGFGSASGSASGGPVLAFRPDPSILDGRHANNGWLQELSKPLTRLTWENVAL
                     ISPAMAQQLGLSNGQVLEIGTGGARVMAPTWVLPGQPAEVVTVHLGFGRTRAGRVGNG
                     VGFDANRLRNADTYHWVNGVTVAKTGKKVKLACTQDHQSMEGRHLVRSTALAEHQKNP
                     HWAHEFGIHDPPRDFTMYPNYEYKDNAWGLAIDLTSCVGCNACVVACQAENNIPVVGK
                     TQVWRGREMAWLRIDRYFEGDLDEPALHYQPVMCQQCENAPCEAVCPVAATIHNHEGL
                     NDMAYNRCVGTRYCSNNCPYKVRRFNFLHYSDPKTPQMKLLNNPDVTVRSRGVMEKCT
                     YCVQRINAARIEAHKVNRPIRDGEIVTACQQACPAEAITFGDINDPNSRVAKLKADTL
                     NYGILTDLNTRPRTSYLARLENPNPDMDHA"
     gene            3344..4732
                     /gene="nrfD"
                     /locus_tag="VF720_11305"
     CDS             3344..4732
                     /gene="nrfD"
                     /locus_tag="VF720_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX7879990.1"
                     /translation="MATKPITEHPDHRGLVAPGHTFATVTDKISAVVLTEKPPLFWLV
                     MFTISFVLVNVLLVAVGYLFYKGIGIWGNNIPVGWAFDIINFVWWIGIGHAGTLISAI
                     LLLLRQKWRTSINRFAEAMTLFAVACAGLFPLVHMGRPWLFYWLLPYPTTFGLWPQFK
                     SPLMWDVFAVSTYATVSLLFWFVGLIPDLATLRDRATNPIAARIYGLLAMGWRGSSQH
                     WARYEAAYLLLAGLSTPLVLSVHTVVSFDFSIGIIPGWHATIFPPYFVAGAIYAGFAM
                     VLTLAIPIRKAFRLEDFITMRHMQNMAWVMLATGMIVVYGYMMELFFAWYSGNIYEMF
                     MMKNRLFGPYAWSYWMLLVCNMLAPQTLWFKPFRTDMRLLFAICMFVNVGMWLERFVI
                     IVTSLHRDFLPSSWGMYYPTIWDFATLAGTIGLFLFLMFSFIKFLPMISISEMRMILP
                     QAVLSKKKEGGH"
     gene            4732..5268
                     /locus_tag="VF720_11310"
     CDS             4732..5268
                     /locus_tag="VF720_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX7879991.1"
                     /translation="MARPPIYGLLAQFATPQALVEAAHKVHEAGFHKVDAYTPYPVEE
                     VIEALHQHDSKVPWLVFLGGCAGAFAGYSMQYFAAVIDYPMNVAGKPTHAWPMYIPIT
                     FELTILLASFSAVFGMLAINGLPMPYHPLFNVPGFKKASQDGFFLCIEARDPRFDRAA
                     TARFLEGLKPLEVHEVAH"
     gene            5258..5926
                     /locus_tag="VF720_11315"
     CDS             5258..5926
                     /locus_tag="VF720_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX7879992.1"
                     /translation="MRIDSGAAVALLALLALTGCHRDMRDQHKYRGYRESSFFADGRS
                     IRPAVPGTIPMGSLKSDTLLYTGKMNGKPVDMFPFQVDRAVLERGRERFNIYCSPCHD
                     FTGSGNGMIVQRGHKKPPSFHEWRLRNAPAGYFFDVITNGFGAMYDYSYQLRPEDRWA
                     VVAWVRTLQTAARGNLADVPADQRGQLDQAAVSPGVPGGHGAPAGHGATPTPAPDPGH
                     GGGH"
BASE COUNT         1006 a         2100 c         1859 g          977 t
ORIGIN      
        1 atccagtgcc acagggcgcc cgagaagttc gtccggccga aggaggccgt gtaccagttc
       61 ggctgggagc ctccggccgg tgagacgcag gccacgctgg gccccaggct ggtggccgag
      121 taccacttga accccaaaac ggactgctcg atatgtcatc ggtgatcgag aaccaggggg
      181 acatggcgcc ccgcgagaat gagccgggcc tgagcctcga ggccatccga acccgcctgg
      241 ccggcaagac cggcaaggtg tactggcgca gcctcgaaga gctggccgat gccccggagt
      301 tcaccgacct gctccaccgg gaattcccgc ggcaggcctc ggagtggaac gaaggcgtga
      361 gccggcgcga cttcctgcac gtgatggcgg cctcgctggc gctggccggc ctttccggct
      421 gcgcgccgcg caacccggcc gagaagatcc tgccgtacgt aaaggcgccc gaagggttca
      481 tccccggcaa gccgctctat tacgccactt ccatcatgga tggaggagcg gccaccggcg
      541 tcctcgtgga aagccacatg ggacggccga tcaagatcga ggggaacgac cgccacccgg
      601 ccagcctcgg cgccaccagc gccccgctcc aggcctccat cctcaccatg tacgacccgg
      661 accgggccca ggtggtcacc aatctcgagg aagtgcgccc ctggaccagt ttcgtcacgg
      721 ccatgacggc atccctggcg gcacagaaga taaccggggg atcgggcctg cgcatcctca
      781 ccgaaaccat caactcgccg accgcggccg cccagctcga gaagatcctg gccgactacc
      841 cggcggccag atggcaccag ttcgagccgc tcaaccgcga caacgtggtg gagggggccc
      901 gtctcgcctt cggcgagccg gtcgacacgc acttcaacct gctcgaggcg cgcgtcatcg
      961 tgtcgctcga cgccgacttc ctcttctccg gcccgggtca tctccgctac gtgcgcgatt
     1021 tcgtgcgcgg tcggcgagtc cggaccccgg gatcggggca cggggccgat tcccacggat
     1081 cggcaacggg agtggctccg ggatccgatc cgcaccaggg gcaggtggaa aaatcgccca
     1141 ccgaatacgg agccgggccc gccgtggcgg aacacggcga gtccccgacc ggcgccggac
     1201 gtgccgcaga gccaccgaag atgtcgatga atcggctcta cgtggccgag agcggtttca
     1261 cgaacaccgg ctcgcgggcc gaccatcgcc tggcggtccg gccggaagtc atcgaatcga
     1321 tcgcgcggga aatcgcgcgg gccctgggaa tcggcgcggc gcccggcggt gcccccctcg
     1381 atgcgaaagc cgagcgctgg gtggccggac tcgtgtccga cctccgcaag aaccccgggg
     1441 cttcggccgt gatggtcggc gagcagcagc cgccggcggt gcacgccctg gcgcatgcca
     1501 tcaacgccgc tctcgggaac gtcggtggcc cggtgctcgt caccgcgccg atcgcggccc
     1561 gcgccgagaa ccagctggcc tcgatcaaga tgctggccga cgacatggaa gccgggcgcg
     1621 tacagctcct tctgatcctg ggaggcaatc cggtctacaa cgcgccgccc gagctcgatt
     1681 tcgcgcggcg gctggggaag gtgggtgtcc gcgtccacca cggattgtac gaggacgaga
     1741 cctccgagct ctgccactgg cacattcccg acacgcatcc tctcgaggcg tggagcgacg
     1801 gccgcgccta cgatggcacg gtgtcgatcg tgcagccgct catcgctccg ctgtacgaag
     1861 gaaagtcgat ccacgaagtc ctggccgtgc tcaccggcga aaccgccaag tcggggtacg
     1921 acctggtgcg cgaccactgg aagagcgtga tgccgggcgc ctcgttcgaa tcgacctgga
     1981 gcacggcggt ccacgacggc cttctcgccg gcagcgcggc ggcacccttc gccggcgcca
     2041 tccgcgacgc ggccggtttc ggcagcgcct cgggttcggc atccggcgga ccggtactgg
     2101 cgttccggcc cgacccgagc atcctcgacg gacgtcacgc caataacggc tggctccagg
     2161 aactctcgaa gccgctgacg cggcttacct gggaaaacgt ggcgctcatc tcgccggcca
     2221 tggcccagca actcggtctg tcgaatggac aggtgctgga gatcggcacc ggcggagcgc
     2281 gcgtcatggc tcccacctgg gtcctgccgg gccagccggc cgaggtggtc accgtgcacc
     2341 tcggattcgg gcgtacccgg gccggccggg tggggaacgg tgtcgggttc gacgccaacc
     2401 ggctccgcaa tgccgatacc tatcactggg tcaacggcgt caccgtcgcg aaaaccggca
     2461 agaaggtgaa gctggcctgc acccaggacc accaatcgat ggaggggcgc catctcgtgc
     2521 gctcgaccgc actggccgag caccagaaga atccccactg ggcgcacgaa ttcggcatcc
     2581 acgaccctcc ccgggatttc acgatgtacc cgaactacga gtacaaggac aacgcgtggg
     2641 gcctggccat cgacctcacc tcctgcgtgg gatgcaatgc ctgcgtggtg gcctgccagg
     2701 ctgagaacaa catccccgtg gtcggcaaga cgcaggtgtg gcgcggccgc gagatggcct
     2761 ggctgcgcat cgatcgctac ttcgaaggag acctggacga gccggccctg cattaccagc
     2821 cggtcatgtg ccagcagtgc gagaatgccc cgtgcgaggc ggtgtgcccg gtggccgcca
     2881 ccatccacaa ccatgagggc ctgaacgaca tggcctacaa ccggtgcgtg gggacgcgct
     2941 actgctcgaa caactgcccg tacaaggtac ggcgcttcaa cttcctgcac tactccgacc
     3001 cgaagacgcc gcagatgaag ctgctgaaca accccgacgt caccgtccgc tcgcgcggcg
     3061 tcatggagaa gtgcacctac tgcgtgcagc ggatcaacgc ggcgcgcatc gaggcccaca
     3121 aggtcaaccg gccgatccgg gacggcgaga tcgtcacggc ctgccagcag gcctgcccgg
     3181 ccgaggccat caccttcggc gacatcaacg atccgaacag ccgcgtggcc aaactcaagg
     3241 ccgacacgct caactacggc atcctgaccg acctgaacac gcgtccccgg acgagttacc
     3301 tggcccggct cgagaacccg aaccccgaca tggaccacgc ctgatggcca cgaagccgat
     3361 caccgagcac ccggaccatc gcggactcgt ggcccccggc cacaccttcg ccacggtcac
     3421 ggacaagatc agcgcggtgg tcctcaccga gaagccgccg ctcttctggc tcgtcatgtt
     3481 caccatctcg ttcgtcctgg tgaacgtgct gctggtggcc gtcggctacc tgttctacaa
     3541 ggggatcggg atctggggca acaacatccc ggtcggctgg gcgttcgaca tcatcaactt
     3601 cgtctggtgg atcggcatcg gccacgccgg tacgctgatc tcggccatcc tgctcctgct
     3661 ccggcagaaa tggcgtacct cgatcaaccg ctttgccgag gcaatgaccc tctttgccgt
     3721 ggcctgcgcc ggcctcttcc cgctggtgca catgggacgg ccgtggctct tctactggct
     3781 gctgccgtac ccgaccacct tcggcctctg gccgcagttc aagagcccgc tcatgtggga
     3841 cgtcttcgcg gtgtccacgt acgccaccgt gtctctcctc ttctggttcg tcggcctcat
     3901 tcccgacctg gccacgctcc gtgaccgggc cacgaacccg atcgccgcgc gcatctacgg
     3961 gctcctggcc atggggtggc gcgggtccag ccagcactgg gcgcgttacg aggcggccta
     4021 tctcctcctg gccgggctct ccacgccgct cgtgctctcc gtccacacgg tcgtttcgtt
     4081 cgacttctcc atcggcatca tcccggggtg gcacgccacc atcttcccgc cctacttcgt
     4141 ggccggcgcc atctacgccg gcttcgccat ggtgctcacg ctggccatcc ccatccggaa
     4201 ggcgttccgg ctggaagatt tcatcacgat gcgccacatg cagaacatgg cctgggtgat
     4261 gctggccacc ggcatgatcg tggtgtacgg ctacatgatg gagctcttct tcgcctggta
     4321 cagcgggaac atctacgaga tgttcatgat gaagaaccgc ctgttcggac cgtacgcctg
     4381 gtcgtactgg atgctgctcg tgtgcaacat gctcgcgccg cagactctct ggttcaagcc
     4441 gttccgcacc gacatgaggc tcctgttcgc catctgcatg ttcgtgaacg tggggatgtg
     4501 gctggagcga ttcgtcatca tcgtcacctc actccatcgc gacttcctgc cgtcgtcgtg
     4561 gggcatgtac tacccgacca tctgggactt cgccacgctc gccggcacga tcggcctctt
     4621 ccttttcctg atgttcagct tcatcaagtt cctcccgatg atctcgatct cggaaatgag
     4681 gatgatcctt ccgcaggccg tcctgtcgaa gaagaaggaa ggaggccact gatggcccga
     4741 ccgccgatct acggcctcct cgcccagttc gccacgccgc aggccctggt ggaagcggcg
     4801 cacaaggtgc atgaggccgg cttccacaag gtggatgcct acacgcccta ccccgtggaa
     4861 gaggtcatcg aggcgctgca ccagcacgac tccaaggtgc cgtggctcgt gtttctgggc
     4921 gggtgcgccg gcgcgttcgc aggctactcc atgcagtact ttgccgccgt catcgactac
     4981 ccgatgaacg tggccggcaa gccgacgcac gcctggccga tgtacatccc gatcacgttc
     5041 gagctcacca tcctcctggc ctcgttctcg gcggtcttcg gcatgctggc catcaacggc
     5101 ctcccgatgc cgtatcaccc gctcttcaac gtgcccggct tcaagaaggc ctcgcaggac
     5161 gggttcttcc tctgcatcga ggcgcgggac ccgcgcttcg accgggcggc taccgccaga
     5221 ttcctcgaag ggctcaagcc gctggaggtt cacgaagttg cgcattgatt ccggcgccgc
     5281 cgtggccctc ctcgcgctcc tcgccctgac cggttgccac cgggacatgc gcgaccagca
     5341 caagtaccgg gggtaccgcg aatcgtcctt ctttgccgac ggtcgctcga tccgtccggc
     5401 cgtgccgggc acgattccga tgggctccct gaagtccgac acgctcctct acaccggcaa
     5461 gatgaacggc aagccggtcg acatgttccc gttccaggtg gatcgggccg tgctcgagcg
     5521 gggacgggag cgcttcaaca tctactgctc gccctgccat gacttcacgg gatccgggaa
     5581 cggcatgatc gtccagcgcg gacacaagaa gcccccctcg ttccacgagt ggcggctgcg
     5641 caacgccccc gccggctatt tcttcgacgt catcaccaac ggttttggcg ccatgtacga
     5701 ctactcgtac caactccgcc ccgaggatcg ctgggcggtg gtggcatggg tccgcacgct
     5761 ccagaccgcc gcccgcggca acctggccga cgtgccggcc gaccagcgcg gccagctcga
     5821 tcaggccgcc gtttcgcccg gtgtgccggg cggacacggg gctcccgccg gacacggcgc
     5881 cacgcccacg ccggcgcccg atccggggca cgggggagga cactgatggc ggccacgctc
     5941 ta
//
LOCUS       DASVOD010000508         4131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_345479, whole genome shotgun sequence.
ACCESSION   DASVOD010000508 DASVOD010000000
VERSION     DASVOD010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345479"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF720_11320"
     CDS             complement(<1..207)
                     /locus_tag="VF720_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012910124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX7879993.1"
                     /translation="MLVRIARGDAQAVKECIDRYEGVVWKIARRLSRSAADAEDGVQE
                     AFIDLWRSASRFDPARSSEQAFVSM"
     gene            419..1477
                     /locus_tag="VF720_11325"
     CDS             419..1477
                     /locus_tag="VF720_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX7879994.1"
                     /translation="MTSREVDFIVGLLDQGGAAYAGLAVRRLFERHPGIGVQHGPDAV
                     ARWRQHMVHRIAELATAVDFESPGLFARAVRWSRHALRTRNLPPVELAGSLDALRDVL
                     REELPEPCRAPIEPCFEAAFDSVEAAPPASPSLDPASERHRLALQYVEACLEGNPIRA
                     TGLILAAVAGGLSIHEACHDVLVPALGEVGRLWHEGRMEIHEEHTVTAVTHRLVGMMA
                     GAAEFGPPNGKTVVAGTVEGDAHDTMLRMLMLEFEMAGWRAIGLGTQLPPAELADSAR
                     DADADLVVISATLIPQLRSLRQGIRLVRERSPRSRILVGGPALDDSPGVRAALEADVI
                     GIESRSVVESGSRLVGLV"
     gene            complement(1495..1938)
                     /locus_tag="VF720_11330"
     CDS             complement(1495..1938)
                     /locus_tag="VF720_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7879995.1"
                     /translation="MRELRRSQVIPVERNRVFEFFADAGNLEEITPSWLRFHVVSPMP
                     IVMKPGVRIEYALRLHAVPIRWISEITEWEPPFRFVDRQVRGPYRLWVHEHRFDALPG
                     GTVVSDHVRYSAPGGPLVHRFLVAPDLKKIFDYRASRISVLLPAS"
     gene            complement(1953..2714)
                     /locus_tag="VF720_11335"
     CDS             complement(1953..2714)
                     /locus_tag="VF720_11335"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX7879996.1"
                     /translation="MTMLKTWARLDWRRQMPTLAALLILSLLAVGCSDDDDDDDNAGE
                     ADIRVMHLSPDAPAVDVYLDGATAPAVSDLLFNQGTGFLAVPAGQHDLAVAPANTSAA
                     SAVLSVSDVELATDAQYTAVAYAPLASIDAMLLVDNLSAPASGQIRVRAIHTASGVGQ
                     VDIWNVPSSGAPTPLYTNVNFGDAGGYLVIPAGSYRLGFDVNNDAVPDLTFTTPSLAA
                     GTVANIFAVSDGSGNVYLLAQLQNSQVVRVNPDPA"
     gene            complement(2739..2954)
                     /locus_tag="VF720_11340"
     CDS             complement(2739..2954)
                     /locus_tag="VF720_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEX7879997.1"
                     /translation="MKKLDVLAAILLVVGGLNWGLVGLFKFDLVAALLGPATVGSAVV
                     YTLVGLCAVYQVFSWKSIQRRWGTAAA"
     gene            complement(3000..>4131)
                     /locus_tag="VF720_11345"
     CDS             complement(3000..>4131)
                     /locus_tag="VF720_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7879998.1"
                     /translation="SHLRSRLEVGEILRASGVPTIEFRASVVIGSGSLSFEMIRTLVE
                     RLPVMVAPRWVGVAAQPIAINDLLAYLVAAVDLPCDANRVFEIGGADETSYGGMMREY
                     ARQRGLRRWIIPVPVLTPRLSSLWLGLVTPLYARVGRRLIDSIRHPTVVRDPSAQSAF
                     DIRPVGFREAIAAAIRHEDASIAETRWSDAHSASGLAAVPVAGPTGRRLFDSRSVEVS
                     ATAESAFRPIERIGGSTGWYYGNWLWRLRGLIDLSVGGIGLRRGRRDPHRLRVGDTLD
                     WWRVEAIDAPRRLRLVAEMKLPGRAWLEFEVSDRPAGGVRVRQLAVFEPVGRLGPLYW
                     YLMAPLHQVIFAGMIQAIARQAAPPENNLREPASDPSPPANS"
BASE COUNT          720 a         1453 c         1316 g          642 t
ORIGIN      
        1 catggagacg aaggcctgtt ccgaggaccg cgccggatcg aagcgcgagg cactccgcca
       61 gagatcgatg aacgcctcct gcacaccgtc ctcggcatcg gccgcggacc gggaaaggcg
      121 ccgggcaatc ttccagacca caccttcgta ccggtcgata cactccttga cggcctgcgc
      181 gtcgcctcgg gcgatgcgga ccagcaacgg ctcatcgttc atgacggcgg cgccgaggca
      241 gcgggatcgg ctgtggcatg atgccaatct aaccgccccg ccgggtcgct gtacacgagc
      301 ggccccacga ggagcccctt gacggctccg ctccgcatcc atacgcacca tccgtgcgaa
      361 ccagccaatg ttatcctgcc ggaatcaggt cgatccggcg ttttccggcg gaggatgcat
      421 gacgagtcgt gaggttgatt tcatcgtcgg cctgctggac cagggtgggg cagcttatgc
      481 cggtcttgcc gtgcggcggc tgttcgaacg gcatccgggt atcggcgtcc agcacggtcc
      541 cgatgccgtg gcccgctggc ggcaacacat ggtccatcgt atcgccgaac tggccaccgc
      601 cgtggacttc gaatcgcccg gactctttgc gcgggcggtt cgctggtcgc gccacgcgct
      661 ccgcacccgc aaccttccgc ccgtcgaact ggccggcagc ctcgacgcgc ttcgcgacgt
      721 cctccgtgag gaactgccgg aaccgtgtcg cgcccccatc gagccctgct tcgaggcggc
      781 cttcgactcg gtcgaagccg cgccgccggc gtcgccatca ctggatcctg ccagcgaacg
      841 tcaccggttg gcgctccagt acgtcgaagc gtgcctggag ggaaatccga tccgggccac
      901 cgggttgatc ctcgccgccg tggccggggg cctctccata catgaagcct gccatgacgt
      961 cctcgttccc gccctgggcg aagtcggacg gctctggcat gaagggcgca tggaaatcca
     1021 cgaggaacac accgtgacgg cggttaccca ccgcctggtc ggcatgatgg cgggtgcggc
     1081 ggagttcggc ccccccaacg gcaagaccgt cgtggccgga accgtcgaag gagatgccca
     1141 cgacaccatg ctccgtatgc tcatgctgga atttgaaatg gcaggatggc gagcgatcgg
     1201 gctcggcacc caactgcccc cagcggagtt ggccgattcc gcgagggatg cggatgccga
     1261 cctggtggtg atctcggcca cgctcatccc tcagttgagg agcctccgcc agggtatccg
     1321 gctggtgcgc gaacgctctc cccgcagccg catcctggtc ggcggaccgg cgctcgatga
     1381 ttcgccgggc gttcgcgccg ctctcgaggc agacgtcatc gggatcgagt cgcggtccgt
     1441 ggtggagtca ggatcgcggc tggtcgggct tgtgtgaacg gccctgcctg cgccctaact
     1501 ggcaggcaga agaacggaga tcctgctcgc ccggtaatcg aagatctttt tcaggtccgg
     1561 ggccaccaga aaccggtgaa caagcggccc gcccggggca gaatagcgaa catggtcgct
     1621 caccacggtt ccaccgggca atgcgtcgaa gcgatgttca tgcacccaga gccggtaagg
     1681 ccccctcacc tggcggtcga cgaaccggaa gggtggttcc cactcggtga tctcgctgat
     1741 ccagcggatc ggcacggcat gaagccggag ggcgtattcg atacgcaccc ccggcttcat
     1801 gacgatcggc atcggcgaca caacgtggaa ccgtagccag gacggcgtga tctcttccag
     1861 gtttccggcg tccgcgaaga attcgaagac gcgattccgc tcgaccggga tgacctgcga
     1921 tcggcgcagt tcgcgcactc cgccttcgtg catcacgccg ggtcgggatt gacccgcacc
     1981 acctggctgt tctggagctg ggccagcagg taaacgttgc cgctcccgtc cgacacggca
     2041 aagatgttcg ccacggtgcc ggcggccagg gacggggtcg tgaacgtgag gtccggaact
     2101 gcgtcattgt ttacgtcgaa gccgagccga taggagcccg ccggtatcac gagataaccc
     2161 cccgcgtcac cgaaattcac gttggtgtag agcggcgtgg gtgccccgct cgacggaaca
     2221 ttccagatgt ccacctggcc cactccggat gccgtgtgaa tggcccgcac ccggatctgc
     2281 ccggaagccg gcgcactcag gttgtcgacc agcagcatgg cgtcgatcga ggccaggggc
     2341 gcgtaggcta ccgccgtgta ttgagcatcc gtggcgagtt ccacgtcgga aaccgacagc
     2401 accgccgagg cggcggaggt attggccggc gccaccgcaa ggtcgtgctg cccggccggt
     2461 acggccagga acccggttcc ctggttgaac aggaggtcgg aaaccgcggg cgccgtcgca
     2521 ccatccaggt acacatccac ggccggagcg tccggcgaca ggtgcatgac ccggatgtcc
     2581 gcctcgccgg cattgtcgtc gtcgtcatca tcatcactgc atccgaccgc caacagcgac
     2641 aggatgagaa gcgccgccag ggtcggcatc tgccggcgcc agtcaagtcg ggcccaggtc
     2701 ttgagcattg tcatcgatac cctttccgct ggcggccgtc aggccgccgc tgttccccaa
     2761 cgcctctgga tcgacttcca cgagaacacc tggtacacgg cgcacagtcc caccagcgta
     2821 tagacgacgg cactgccaac cgtggccggg ccgagcagcg ccgcaaccag gtcgaacttg
     2881 aagaggccca ccaggcccca gttcaagcca ccgaccacga gcaggatcgc cgcgagcaca
     2941 tcaagctttt tcatcacgat ctcctgtggg tccgcgaagc gccccgtgcg cttcgctgct
     3001 taactgttcg caggcggtga aggatcggat gcaggctccc gcagattgtt ttccggtggc
     3061 gcggcctggc gggcgatggc ctggatcatc ccggcgaaga tgacctggtg gaggggggcc
     3121 atgaggtacc agtagagggg cccaagccgg cccaccggtt cgaagaccgc cagttgccgg
     3181 acccggacgc cgcccgccgg ccgatcggac acctcgaact ccagccatgc ccgtcccgga
     3241 agcttcattt cggccaccag ccgcagccgc cggggcgcat cgatggcctc cacccgccac
     3301 cagtcgagcg tatcgcccac ccgcagccgg tgcggatcgc gccgcccccg cctgagcccg
     3361 atacctccca ccgagaggtc aatcagcccc cgcaggcgcc agagccagtt gccgtaatac
     3421 cagccggtcg atccgccgat ccgctcgatg ggccggaacg ccgactccgc cgtggcgctc
     3481 acctccacgc tccgcgagtc gaagagccgc cggcccgtcg gtcccgccac cgggaccgcg
     3541 gcgaggccgc tcgcggagtg cgcatccgac cagcgcgttt ccgcgatcga cgcatcctcg
     3601 tggcggatgg ccgcggcgat ggcttcccgg aagccgacgg gacggatgtc gaacgccgac
     3661 tgcgcggacg gatcccgcac caccgtcggg tgccggatgc tgtcgatcag ccgccttccc
     3721 acccgcgcat acagcggcgt gaccagcccc agccacaggc tggagagccg cggcgtcaac
     3781 accggaaccg ggatgatcca gcgccggagg ccgcgctgcc ttgcgtactc ccgcatcatc
     3841 ccgccgtacg aggtttcgtc ggccccgccg atctcgaaca cgcggttcgc atcgcatgga
     3901 aggtcgacag ccgcaaccag gtacgccagg aggtcgttga tggcgatcgg ctgggccgcc
     3961 acccccaccc agcgcggcgc caccatcacc ggcaaccgct ccacaagggt ccggatcatc
     4021 tcgaacgaca ggcttcccga tccgatcacc accgaagccc gaaactcgat cgtcggcacg
     4081 ccactcgccc gcaagatttc gccgacctcc aaccgactcc gcagatggga c
//
LOCUS       DASVOD010000509         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_345556, whole genome shotgun sequence.
ACCESSION   DASVOD010000509 DASVOD010000000
VERSION     DASVOD010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345556"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1566)
                     /locus_tag="VF720_11350"
     CDS             complement(145..1566)
                     /locus_tag="VF720_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7879999.1"
                     /translation="MIRRAGRGGWDGWQRSAGTIVVLGIVALVCAWMVTRRAEAQSGE
                     PGERPEQEVTLSLEFTGQTKLNLLLEEFSAEQGTTKSDADKTRGVVQNDLALTDLFNL
                     VTVRANLGMAPPADTIGWAAYRPQDGSIFTGISDRGYPNASFVAGGKVRLNGSDLVLD
                     AWLKEYPSTRDILTRTYRVRPEWYREAAHRFSDDIVLYLTGIEGISRTRIAFISDASG
                     KKELYLTDFDGENVRQITRDKSIALSPAWSADGGRVGFVSFRKGDADLYAVNLGSGQI
                     TAMVTRPGPEIGPSFSRDGRRLAWSSTVEGQSEIYSSGPDGASPKRLTTNRAIDTAPA
                     WSPTGNEVVFTSDRSGNPQLYVMDAEGGNVRRLSWQGNWNDSADWSPDGLRLVYVSRQ
                     GGAFKIWSMLADGGGPVAMTTGAGTDENPKWAPDGRKIVFSSTRQGRRALYTMNSDGS
                     GVRRLTFLDADCYGTSWGPRLPR"
     gene            complement(1580..>1793)
                     /locus_tag="VF720_11355"
     CDS             complement(1580..>1793)
                     /locus_tag="VF720_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX7880000.1"
                     /translation="PGKRAVALVTFRIQRSGTVSKSSLEEPSGRRDFDEAALRAIIDA
                     QPFAPLPASFGGQFLTVNFQFAYQGQ"
BASE COUNT          281 a          626 c          586 g          300 t
ORIGIN      
        1 ggccgcgatc acgagaagag gcagaaaccg cgagaccggt cgaatcgtcg tcatcgttca
       61 aatctccttt gcgaaacagg actgcgtgtc gttcgtttca gtctaggcgg tcggcgtcgt
      121 gtcgatcaac ccgtgcgaga cggatcagcg gggcagacga ggtccccacg acgtgccata
      181 acagtccgcg tcgaggaacg tgagccggcg cacgcccgac ccgtcggaat tcatcgtgta
      241 gagcgcgcga cgcccctggc gcgtgctcga gaagacgatc ttgcgcccgt cgggcgccca
      301 tttcggattt tcatcggtgc ccgccccagt ggtcatggcc accgggccac cgccgtcggc
      361 cagcatcgac cagatcttga acgcgccccc ctgccgcgac acgtacacga gccgcagccc
      421 gtcgggcgac cagtcggccg agtcgttcca gttaccctgc caggagagtc ggcgcacatt
      481 acccccttcg gcatccatca cgtacagctg gggattcccc gaacgatcgc tggtgaaaac
      541 cacctcgttg ccggtgggcg accacgccgg ggcggtgtcg atggcccggt tggtggtgag
      601 gcgcttcggg gaggccccat ccggccccga cgagtagatc tcgctctgcc cctccactgt
      661 ggacgaccag gcgagccgcc ggccgtcccg gctgaacgag gggccgatct cgggcccggg
      721 cctggtcacc atcgcggtga tctggccgct gccgaggttc acggcgtaga ggtcggcatc
      781 tcccttgcgg aacgacacga accccacccg cccgccgtcg gcactccagg ccggcgagag
      841 tgcgatcgac ttgtcccgcg tgatctgccg gacgttctcg ccgtcgaagt cggtgaggta
      901 gagctccttt ttccccgacg cgtcggagat gaaggcgatg cgcgtccggc tgatcccctc
      961 gatgccggtc aggtagagga cgatgtcgtc gctgaagcgg tgggccgcct cgcgatacca
     1021 ctccggccgc acccggtagg tgcgggtcag gatgtcccgg gtgctgggat actccttgag
     1081 ccaggcgtcg aggacgaggt cgctgccgtt caggcgtacc ttgccccccg ccacgaaact
     1141 ggcgttggga tagccgcggt cggagatgcc ggtgaagatg ctgccgtcct gcgggcggta
     1201 ggcggcccag ccgatcgtgt cggcgggggg ggccattccc aggttggcgc gcaccgtgac
     1261 gaggttgaag aggtcggtca gcgccaggtc gttctgcacg acgccccgcg tcttgtccgc
     1321 gtcggacttc gtggtcccct gctcggccga gaattcctcg agcaggaggt tgagcttggt
     1381 ctgccccgtg aactcgaggc tcagggtcac ttcctgttcc ggccgctcac ccggctcgcc
     1441 ggactgcgct tccgcccgcc gcgtgaccat ccatgcgcac acgagggcca cgatcccgag
     1501 caccacgatc gtgcccgcgc tccgttgcca cccgtcccat ccaccccgcc cggcgcgtcg
     1561 aatcaccaat ctcctccgat tactggccct ggtacgcgaa ctggaaattg accgtcagga
     1621 actgcccgcc gaacgatgcc ggcagcggcg cgaacggctg cgcatcgatg atggcacgca
     1681 acgccgcctc gtcgaagtca cgccggcccg acggctcctc gagcgacgac ttcgagactg
     1741 tcccgctccg ctggatccgg aaggtgacga gcgccaccgc ccgctttccg ggc
//
LOCUS       DASVOD010000510         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_139277, whole genome shotgun sequence.
ACCESSION   DASVOD010000510 DASVOD010000000
VERSION     DASVOD010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139277"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /locus_tag="VF720_11360"
     CDS             complement(<1..1637)
                     /locus_tag="VF720_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX7880001.1"
                     /translation="MRNVCLNLVSEFSTERGTASVSALVDRARQARVDIVGLVDRATL
                     IGAAPFLAEARRRELGAVVGVEFEVASMAPAPASLESLTAPDTYPLVLIAESAEGRAN
                     LTRLVSLAHSRAGGGGMGQPAAGAFPSSVGRPGVGRPFLLVEDLLAHHHGLIAQSPGL
                     VGEVSSRIQRGQADLAERLALRLVEVFGRSAVYLGVSLHGLPAEARHAAEVAALAHRV
                     GVPIVASDLVDHLGEDDRATRNARLALSLDRPLVQVARDRFIAPDSRFRTPSERRRLY
                     RDLPEALDNEVALAERAIVRDEVPVGSGLRPGTREAPAIGHYPLPVGRTADDYLASIV
                     EVRCPREARYRSRLDEELAESARRGLAGWFLIQWDACRYGRRHGLALEPAGGALRSSL
                     IAHFLGLTDVDPVAVGLAAAHFFIEADAGAPDMIFRLPAGQRAELGAYLEQRYGKERV
                     RRPGEVERISPRAAVRLAGTVLGIAETDVAAAARHVTDAPGGDVAVAVARDEHLARRY
                     VRDPIVRDWLDLARGLSGLVGRIEADRHALVLDAGGQ"
     gene            1802..>2253
                     /locus_tag="VF720_11365"
     CDS             1802..>2253
                     /locus_tag="VF720_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7880002.1"
                     /translation="MSTEIHGWRGLALLLTTLIAIGCDSDSNPDVEAPGAVDDLLVVA
                     RGDTAAFLTWTARGDDGNDGRAAAYDIRYSLENLTATSFDSAIAILSPPMPGMSGDPD
                     SVVIAGLLPDRTYFFGLRSTDEVGNLSDLSNIDSLRTPASPDTMAADVT"
BASE COUNT          414 a          782 c          750 g          307 t
ORIGIN      
        1 cactggccac cggcatcgag gaccagcgcg tgacggtccg cctcgatgcg gccgaccagg
       61 cccgacaacc cgcgcgcgag gtcgagccag tcgcgaacga tcgggtcgcg gacgtagcgg
      121 cgtgccaggt gctcgtcccg cgcaacggca acggccacat ctccacccgg tgcgtcggtg
      181 acatgccgtg ccgcggcggc cacgtcggtt tcggcgatgc cgagaacagt accggccagg
      241 cgcacggcgg cgcgcggcga gatgcgctct acctcgcccg ggcgacggac gcgttccttg
      301 ccgtaacgct gctcgagata ggccccgagc tcggcccgct gtcctgccgg aagccggaag
      361 atcatgtcgg gagcgccagc atcggcctcg atgaaaaagt gcgccgcggc caggccgact
      421 gccaccggat cgacatccgt gaggccgaga aagtgggcga tgagcgagga gcgcagcgcc
      481 cctcccgccg gctccagggc caggccgtga cgcctgccgt aacggcaggc atcccactgg
      541 ataaggaacc atcccgccag gccacgccgg gcggattcgg ccagttcctc gtccaggcgg
      601 ctgcgatagc gcgcctcacg gggacagcgg acttccacga tcgacgcgag ataatcatcg
      661 gcggtccgac cgaccggcag cggatagtga ccgatggccg gcgcctcacg ggtgcccgga
      721 cgcaacccgg agccgacagg cacctcgtca cgaacgatcg cccgctcggc cagcgccacc
      781 tcgttgtcca gcgcctccgg aaggtcccgg tagagccgcc gcctttcgga aggcgtgcgg
      841 aaccggctgt cgggcgcgat gaaacgatcg cgcgccacct gcaccagggg gcgatcgagc
      901 gacagggcca ggcgtgcgtt gcgggtggcg cgatcgtcct cgcccaggtg atcgacgagg
      961 tcggaagcca cgatcggcac gccgacccga tgcgccaggg ccgccacttc agcggcgtgc
     1021 cgtgcctcgg ccggcaggcc gtgcagcgac acaccgagat acacggccga ccgcccaaag
     1081 acctcgacca gccggagtgc cagccgctcc gccagatccg cctgaccccg ctggatccga
     1141 ctcgagactt ccccgacaag accggggctc tgggcgatca gtccgtgatg atgcgcgagg
     1201 agatcctcga caaggaggaa cggtcggccg acgccggggc gtccaacgga agacgggaac
     1261 gccccggccg ccggctgacc catgcccccc ccgccggcgc gggaatgcgc gagggagaca
     1321 agtcgcgtga ggttggcccg tccctcggcc gactcggcga tgaggacgag cggatacgtg
     1381 tcgggtgccg taagggactc gagcgacgcc ggggcgggtg ccatggaggc cacctcgaac
     1441 tcgacgccca cgacggcgcc aagttcgcgg cgccgggctt cggccaggaa gggcgcggct
     1501 ccgatgagcg tggcgcgatc gacgagtccc acgatgtcga cacgcgcctg gcgggcccga
     1561 tcaaccaggg ccgaaacgga ggccgtgccg cgctccgtcg agaattcgct caccaggttc
     1621 aggcagacgt ttcgcatgag tcgaaactac tacacagatg gatgggagtc aaataaatag
     1681 tttcgaatcc ggagggggct cgaaccgatc ggaaaaaact tctatatgcc caataagaaa
     1741 gcagttagca tcaactgcac tgccacgaac gttcgacgcc acccccgctt gcggagccgc
     1801 agtgagcacc gaaattcacg gatggcgcgg gctcgccctc cttctcacga ccctgattgc
     1861 gatcggctgc gactccgact cgaacccgga cgtggaggca cctggtgcgg tcgatgacct
     1921 cttggttgtg gcacggggtg atacggccgc cttcctgacg tggacggcgc ggggggacga
     1981 cggaaacgac ggcagggctg cggcgtacga catccgctac agcctcgaga acctgaccgc
     2041 caccagcttc gattccgcca tcgccatcct ttcgcccccg atgcccggaa tgtccggcga
     2101 cccggactcg gtagtcattg ccggattgct gcccgaccga acctacttct tcggtctccg
     2161 ttccacggac gaggtcggca atctctcgga cctttccaac atcgattcgt tgagaactcc
     2221 agcctcgccc gacacgatgg cagccgacgt cac
//
LOCUS       DASVOD010000511         4747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_36005, whole genome shotgun sequence.
ACCESSION   DASVOD010000511 DASVOD010000000
VERSION     DASVOD010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36005"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VF720_11370"
     CDS             <1..928
                     /locus_tag="VF720_11370"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HEX7880003.1"
                     /translation="ADIVRAIDWLRPEGSTNVQAGLREGYRMAYEAMRPGRVNRIILC
                     SDGVANNGLTEGDAILDEVRREARRGIELTAVGFGMSNYNDVLMEKLADHGDGSYWYV
                     DRLDEARRIFVQSLTGTLQSVARQAKVQVEFDPARVERYRLIGYENRDVADRDFRNDA
                     VDAGEVGAGHEVTALFELKARDRKASVVAPDGTGALATVRIRYEDPDTRKVREVARSV
                     FPADVLSEERSLPPSLALDAAVAEFAEVLRHSYWSRESTPGDALALARRAVGRLGKRE
                     DRLELIGMMETADRLWPGERRQGWRDDKPGGE"
     gene            complement(1273..1734)
                     /locus_tag="VF720_11375"
     CDS             complement(1273..1734)
                     /locus_tag="VF720_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880004.1"
                     /translation="MRTPIDISRTWLPLVLAGLLVLAGCAKKQENTAQNPPPANNMPG
                     ALHVMSVTLGRTLNPDKRVAEATEQFSPAETVYASVETMGSSPSATLAARWTFEDGQL
                     VDETSQTIAPDGQAVTEFHVTKPSGWPPGSYKLEIMLDGKTAATKSFTVKA"
     gene            complement(1734..2051)
                     /locus_tag="VF720_11380"
     CDS             complement(1734..2051)
                     /locus_tag="VF720_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007512329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7880005.1"
                     /translation="MGLLDKILGRKTPTKPADFSDVRGGSSSTALPPRAHAPAPSSPP
                     VPRTYTVVAGDSLSKIAKREYGDANKWPHIFDANREKIKNPDLIHPGQVLVIPPLTNV
                     GGR"
     gene            complement(2212..3063)
                     /locus_tag="VF720_11385"
     CDS             complement(2212..3063)
                     /locus_tag="VF720_11385"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7880006.1"
                     /translation="MMRALLKSLGASLLGVLALVFVITLVAPARGRPAASGGSLAERV
                     RSIGTGEAYFSFDTWPNVWGDEDCNITRGDPDGEHRAHRIINGHWGSGDKMTQGPMTV
                     RLRVWKGNVTKLENCVGDAWRNGDGDDGVDLGRVTQAEAGAYLVELARKGGGSDKVGS
                     EAIVAAVFVKDADIWPGLFAIARDRKLNDEVRSSALFWIGQEAGERAVGELVGVAEAD
                     DEESDVRESAVFALSQRPSEESIPVLTRLARTSEHPEVRKSAMFWLAQHDDPEVIDFF
                     EEILSAH"
     gene            complement(3060..4523)
                     /locus_tag="VF720_11390"
     CDS             complement(3060..4523)
                     /locus_tag="VF720_11390"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7880007.1"
                     /translation="MITRPTRHLAHWALAAMLAIGLPTAARLVQPAPAWAMPDKEDAE
                     DVDDPAEPAYDAAHDLLDEEEYEDAVAAFDKLVAKYPKSPLAGESLYWKAFALFRLED
                     SEHLQNARKVLLEQGKVYPKAATRRDAKDLLIRIDGQLARMGDSEAAARLAAEGKRMS
                     QDDAVREARAIARAEADAARGKMHDVRGTHAYDDDDDDMDTQMAALQALMQMNPERSI
                     PILRKILARRDARSAELRSHALFLLGQIQTPESEAMLLDAVRNDPDIEVKKNGVFWLS
                     QNPSEASLDAILEILRTSPTDEVQESSVFALSQHPSPRAREALRDLALNPKATHHVRE
                     QAIFWIGQDSSDSSIEFLKAIYRKTTEDDLKEKILFSISQQGSEASESWLLERARDPN
                     ETMDVRKNAIFWLGQTGALDSVELKEIYRTCDDSEMKQQIIFVASQQGTRASVDLLIE
                     IARNDTDKEMRQQAIFWLGQSGDEKATDYLESLLGGE"
     gene            complement(4520..>4747)
                     /locus_tag="VF720_11395"
     CDS             complement(4520..>4747)
                     /locus_tag="VF720_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880008.1"
                     /translation="VRLLLDTTGSNDPEMKSLLLDLEVVLAQILQASADGSTLDRDLA
                     TRRLNDRAVLPRLRNMIPAGPEAAATQGVPR"
BASE COUNT          723 a         1552 c         1640 g          832 t
ORIGIN      
        1 ggccgacatc gtgcgggcga tcgactggct gaggccggaa gggagcacca acgtgcaggc
       61 cggcctgcgc gaggggtatc gcatggcata tgaggcgatg cggccgggtc gtgtgaaccg
      121 catcatcctc tgttcggacg gcgtggccaa caatggcctc accgaaggag atgcgatcct
      181 ggacgaggtg cgacgcgagg cccggcgagg gatcgagctc accgccgtcg gtttcggaat
      241 gagcaattac aacgacgtgc tcatggaaaa gctggccgac catggtgatg ggagctactg
      301 gtatgtggac cggctcgatg aagcccgccg gatcttcgtg cagtcgctga cgggcacgct
      361 tcagtcggtg gcgcggcagg ccaaggtgca ggtcgagttc gatccggcac gcgtggaacg
      421 ctaccggctc atcggctacg agaaccgcga cgtggccgat cgcgacttcc ggaacgacgc
      481 cgtggatgcc ggcgaggtgg gcgccgggca cgaggtgacg gcgctcttcg aactcaaggc
      541 gcgggaccgg aaggcctcgg tcgtggcgcc cgacggcacc ggcgcgctgg ccaccgtgcg
      601 catccgctac gaggatccgg acacgcgcaa ggtgcgcgaa gtggcacgaa gcgtgttccc
      661 cgccgacgtg ctgtccgagg aacgttcact cccgcccagc ctcgcgctcg acgccgccgt
      721 cgccgaattc gccgaggtcc tgcgccactc gtactggtcg agggaaagca ccccgggcga
      781 tgccctcgcg ctggcgcgcc gggccgtcgg ccggctcggc aagcgcgagg atcgtctcga
      841 gttgatcggg atgatggaga ccgccgatcg cctgtggccg ggcgaacgcc ggcagggttg
      901 gcgggacgac aagccgggcg gcgagtagca actccggccg gaccgcctgg agcggtgctt
      961 gaagaggtcc ggcgcaccgg ccgttcggtg cggatcacac gtcacggacg gcccgctgcg
     1021 gaagtcaccc cggtgagcgc cgacgtggcg cccgggtcgt ggctcggatg catgaaggat
     1081 gagttccgca tcgtcggcga cattgtttcg ccgatcgtgg cgccggagga gtgggaggtg
     1141 tcgagggatg aatctgctgc tggacaccca cgtgtgggtg tggggcgtaa acgagccggg
     1201 acggattcgc gcggccgcac gcgagcgact cgaaaatcac gatagccgcc cgtggctctc
     1261 ccctcctgac ccctacgcct tcaccgtgaa cgacttcgtc gccgccgtct tcccgtcgag
     1321 catgatctcc agcttgtagc tgccgggagg ccagccgctc ggtttcgtca cgtgaaactc
     1381 cgtgacggcc tgtccatcgg gcgcgatggt ctgcgatgtt tcgtcgacca gttggccgtc
     1441 ctcgaacgtc cagcgggccg ccagtgtggc gctcggcgac gaacccatcg tctccaccga
     1501 ggcgtacacg gtctcggcgg gggagaactg ctcggtggct tcggccacgc gcttgtcggg
     1561 gttgagcgtc cgcccgagag tcacgctcat cacgtgtagc gcgccgggca tgttgttcgc
     1621 cggcggagga ttctgcgccg tgttttcctg cttcttcgcg cagccggcca ggaccaggag
     1681 accggcaagc acgagcggca gccaggtccg ggagatatcg atcggggtgc gcatcaccgg
     1741 cctccgacgt tcgtgagcgg cggtatgacc agcacctggc cgggatggat caggtcggga
     1801 ttcttgatct tctcgcggtt ggcgtcgaag atgtgcggcc acttgttggc atcgccgtat
     1861 tcacgcttgg cgatcttcga gaggctgtcg ccggccacca ccgtgtaggt tcgcggcacc
     1921 ggcggtgagg agggcgccgg ggcatgggct cgcgggggaa gggccgtcga agagctgccg
     1981 ccccggacgt ccgagaaatc ggcaggcttg gtgggagttt tcctcccgag gatcttgtcg
     2041 agcagtccca tggatgtctc ctggcggcca ggtatgcgta ccggcgttcg gatccatccc
     2101 catgattctt ccggggacgc ttccccgggc cgtacccgca atgaccctag cgcaccgggc
     2161 ggggggcgtc catcggagca cgccccatcc cccggccctc cgaaggcccc gtcaatgggc
     2221 cgagaggatt tcctcgaaga agtcgatcac ctcggggtcg tcatgctggg ccagccagaa
     2281 catggcggac ttccgcacct cggggtgctc actggtcctg gccagccggg tgagcacggg
     2341 aatcgattcc tcgctgggac gctgcgagag ggcgaacacc gccgactcgc gcacgtcgga
     2401 ttcctcgtcg tctgcttcgg ccactccgac cagctcaccg acggcccgtt cgccggcttc
     2461 ctgcccgatc cagaagagcg cgctcgagcg gacctcgtca ttcagcttcc gatcccgggc
     2521 gatggcaaag agccccggcc agatgtcggc gtccttcacg aacacggccg ccacgatcgc
     2581 ttcggacccg accttgtcac taccgccacc cttcctggcc agctcgacca ggtaggcgcc
     2641 cgcctccgcc tgcgtcacgc gcccgaggtc gactccgtcg tctccatcgc cattcctcca
     2701 cgcatcgccc acgcagttct cgagcttcgt cacgtttccc ttccagacgc ggagtctcac
     2761 ggtcatgggc ccctgggtca tcttgtcccc cgacccccag tggccgttga tgatgcggtg
     2821 cgcgcggtgt tcgccgtccg ggtcaccgcg ggtgatgttg cagtcctcgt cgccccagac
     2881 gttgggccag gtgtcgaacg agaagtacgc ctcacccgtg ccgatcgaac ggacgcgttc
     2941 ggcgagcgaa ccgcccgagg cagccggtcg gccacgggcc ggggcgacga gggtgattac
     3001 gaagaccagg gcgagcacgc cgagcaggct ggcgccgagc gacttgagga gggcacgcat
     3061 cattcacctc caaggagcga ttccaggtag tccgtggctt tctcatcacc ggactgccca
     3121 agccagaaga tggcctgctg ccgcatctcc ttgtccgtat cgttccgggc gatctcgatg
     3181 agcaggtcga ccgacgcccg ggttccctgc tgcgaggcca cgaagatgat ctgctgcttc
     3241 atttccgagt cgtcgcaggt gcggtagatc tccttgagct cgaccgagtc gagggcgccc
     3301 gtctggccga gccagaagat ggcgttcttc cgcacgtcca tcgtctcgtt cggatcgcgg
     3361 gcccgctcga ggagccacga ttcggacgct tcggacccct gctgggagat cgagaagagg
     3421 atcttctcct tgaggtcgtc ttcggtcgtc ttcctgtaga tggccttgag gaactcgatg
     3481 ctcgagtctg atgagtcctg cccgatccag aagatggcct gctcgcggac atgatgcgtg
     3541 gccttcgggt tgagcgccag gtcgcgaagg gcctctcttg cccgcggact cgggtgctgg
     3601 gagagagcga agacactgct ctcctgcacc tcgtcggtgg gcgacgtccg gaggatctcg
     3661 aggatagcgt ccagcgacgc ctcgctcggg ttttgcgaca gccagaagac gccgttcttc
     3721 ttgacctcga tgtccgggtc gttccgcacc gcgtcgagga gcattgcctc ggactccggg
     3781 gtctggatct ggcccagcag gaacagggcg tgcgagcgca gctcggccga gcgggcgtcc
     3841 cgccgggcca ggatcttccg gaggatcggg atcgatcgct ccggattcat ctgcatgagg
     3901 gcctgcagcg ccgccatctg ggtgtccatg tcgtcgtcgt cgtcgtcgta cgcatgggtc
     3961 ccgcggacgt cgtgcatctt gccgcgcgcc gcatcggcct cggccctggc gatggcacgc
     4021 gcctcccgga cggcatcgtc ctggctcatc cgcttcccct ccgccgcgag ccgcgcggcg
     4081 gcctcggaat cacccatccg cgccagctgg ccgtcgatgc ggatcaggag gtccttcgcg
     4141 tcgcgacggg tggcagcctt cgggtagacc ttgccctgtt cgagcagcac cttgcgggcg
     4201 ttctgcaggt gctcgctgtc ctcgaggcgg aagagcgcga aggccttcca gtagagcgac
     4261 tcgcccgcca ggggcgactt cggatacttc gcgacgagct tgtcgaaggc cgcgacggcg
     4321 tcctcgtact cctcctcgtc gagaaggtca tgggccgcgt cgtaagcagg ctcggccgga
     4381 tcgtccacgt cctcggcatc ttccttgtcg ggcatcgccc aggcgggcgc cggttggacg
     4441 agccgggcgg cggtcggcag gccgatggcc agcatcgcgg ccagcgccca gtgcgccagg
     4501 tgtcgggtgg gtctcgtgat catcgtggaa ctccctgggt ggcggccgct tcaggtccgg
     4561 ccgggatcat gttgcgaaga cggggcagca cggcccggtc attgagacga cgggtggcca
     4621 ggtcgcggtc gagcgtggaa ccgtccgccg aggcctggag gatctgcgcc agcacgacct
     4681 cgagatcgag caggagcgac ttcatttccg gatcgttcga accggtcgtg tcgaggagca
     4741 ggcgcac
//
LOCUS       DASVOD010000512         8795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_920284, whole genome shotgun sequence.
ACCESSION   DASVOD010000512 DASVOD010000000
VERSION     DASVOD010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920284"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="VF720_11400"
     CDS             <1..1217
                     /locus_tag="VF720_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX7880009.1"
                     /translation="NAWTMPIKARIDMLSTGVRTPVGIKVFGSDLATIETIATRIEAV
                     VREVPGARSVFAERAAGGWFLDIEPDREALARHGLTLESLQETIASAVGGENVTTVLD
                     GRARYPVNIRYPREQRQDPDAIGRILVATGHGAQVPLSQLARVAVVSGPSMIRNESGF
                     LAGYVYVDVADRDVGGFVHEAREKVRKEVEMPAGYSLKWSGQYENMQRVQDRLRLVVP
                     ITLLLIALLLYFNTRSMFKTVLILVAVPFSAIGAIWLFHFLDYDVSIAAWVGMIALLG
                     LDAETAVFMLLFLDLSWKEARERGALNSIAEVDEAIRHGAVARVRPKMMTVAAAFMGL
                     LPILWSTSAGADVMKRIAAPMIGGLVTSFAAELLLYPAVYKLWKLRTECRHLRYSPGR
                     SRDPISSERITR"
     gene            1214..1939
                     /locus_tag="VF720_11405"
     CDS             1214..1939
                     /locus_tag="VF720_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880010.1"
                     /translation="MTLRLPLPFQPAHRTLILGAATLLVLGAPWAFRTDARAADKPAG
                     VVAGSDKATAGDVASAPYFLPTDPVTGEKLPAKPVVATIGGREFRFASEANRKTFEAT
                     PDKYVSKVDKAMIEAEKPFYPTRECLVSNEELGSMGEPIDRIVGNRLVRLCCKSCVDK
                     LDKNPATVVKALDAATVKAQKAAYPLKSCVVSGEPLGAMGDPIDMVVGGRLVRLCCKG
                     CVDKLNADPVAFLARVPAAGKKS"
     gene            complement(2062..2607)
                     /locus_tag="VF720_11410"
     CDS             complement(2062..2607)
                     /locus_tag="VF720_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880011.1"
                     /translation="MTIASGPTDPASPVSLAGRPRRFTALLAVLGLALVVVATSSCGD
                     DEDDDDGGSRCGTEALPISDHADGPVLTDVALECQGGNFLNLFATVTDPQGSADLEDV
                     DQRLVVYGQSDCKGDVEFEVMDDISESGGEESFGIVITRTGHEALFDQICAAPEWPVE
                     IHLYDKSGHHTFGKAMARVID"
     gene            complement(2827..3135)
                     /locus_tag="VF720_11415"
     CDS             complement(2827..3135)
                     /locus_tag="VF720_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEX7880012.1"
                     /translation="MREYRSNSEGVMLGIVVGAVLGAGMALLLAPATGTETRRRLGDA
                     ARRVGGQARNRLDDMGGRVKEGMSQVGDKVKDVTSHLKRDVRDAVDAGRDAWESEGNI
                     "
     gene            complement(3128..3550)
                     /locus_tag="VF720_11420"
     CDS             complement(3128..3550)
                     /locus_tag="VF720_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX7880013.1"
                     /translation="MTPLIQGCIVALTAGLIILIVVAVVTMRRLNHTLDNVDRSLLPL
                     EGLITDATRASAELRELLVSLDDVVDRVDGIVGDFKGVSERGARISGALLDEVEGPAR
                     KVTALVRGIKAAGSVLASRWGRNRLKNYSITEGGNGYA"
     gene            complement(3840..4337)
                     /locus_tag="VF720_11425"
     CDS             complement(3840..4337)
                     /locus_tag="VF720_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7880014.1"
                     /translation="MPANERTKGVRPSARSERQVLIDALNDDLSREYQAIIAYVVYSQ
                     VLKGAEYMAIAGELEIHAGEELQHAIRVARQIDYLGGTPITVPKPVKISDEPKALLRF
                     DLENETETIRNYRERVRQAEALEEYALAEELRQILVQEQEHAIDLATALGIEVPPIGK
                     GSTRG"
     gene            4455..5396
                     /locus_tag="VF720_11430"
     CDS             4455..5396
                     /locus_tag="VF720_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7880015.1"
                     /translation="MRGIDIPLPRWLYHVGIALRGTMRLWVSHRADRLAAALAFYAVF
                     SMAPLLVIVIAVAGQVFGPEAVRGEIVAQFGSLIGVEAARQVEVIIQGASNPASGLIA
                     ALIGGLSLMAGATAVFAQLKEALNAIWDAPPLERGFLKTVFRDRIRSLAMVFGVAFLL
                     LVSLVLSAALAALTRWAAPYLPHFPFSLAGTIDFVLSFILVSVLFAMIYRILPDVAIS
                     WGDVAIGAAVTAVLFNLGKYLIGLYLGQSTLGSVFGAAGSLAILMLWVYYSAAVFLLG
                     AGFTRVYSLTWGSLSRKASGTSVTTVEGPASLLSARR"
     gene            complement(5300..6475)
                     /locus_tag="VF720_11435"
     CDS             complement(5300..6475)
                     /locus_tag="VF720_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880016.1"
                     /translation="MTASPGSSRTAAVVGLLVAITSALLVAVFLHRPHGRFADSGAAE
                     LPDSLRVPIESRFRVAWVAAGPSKPRTDFSGIVVPTGPACVDSVQRGRHFEFWLADDK
                     SNRIESMIIRARPAGEGSPRTLLLSPMAILNPRRLPFGDDMEDLARLTGPCNRPVWVA
                     GASEAGPDGESVSWLHLFEERDGRLTLRWTGRGSPPGETVGNNGFEGVAMMRLGADTL
                     VVLAFKERPDPTYVRSFLFTEKQGTAGPIVAAPAAGTDSVSLTPIEPIRMDRIDGLLT
                     QAGACFGPSGDLYVLDRLARRIAVVDRKGIEAAVRDSSENRVSVREWLDFDAIESTLE
                     NKRDPDSPLSPFGTAEGIAFGPDGTLYLLADNNDAGPSTVVTLVPLAFRDRLPQVNE"
     gene            complement(6472..7890)
                     /locus_tag="VF720_11440"
     CDS             complement(6472..7890)
                     /locus_tag="VF720_11440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7880017.1"
                     /translation="MTDERLPVSGPGVLIIDDERSLRFTIGEALRGEGFRVFEAPGGE
                     SGLMQLREEEIDLVLLDQKLKESGEDGIEILKAIKKEWPDMEVVMMTAFGRFESAVEA
                     TKLGCYQYLGKPFEIDQLVLVLRAALENRTLRRQVEFLQRTESGRHHLELVEGKSPAM
                     KRAVDEIDRIAGAATTALLLGETGVGKELLARRIHEKSPVAAGQFVEVNCSSFPENLI
                     ETELFGHEKGAFTDAKGRKKGLLDLADGGTLFLDEIGEMPQSLQPKLLRVLETRKFKR
                     VGGVADVVVNVRFVAATHRDLKKSVADGGFREDLYYRLSVVPVQIPPLRERQDDIPQL
                     VAHFIKHFNRELGRSVTGVDDRAMALMLAYPWPGNVRELKNVIERALLLHDASAIAAE
                     HLPSELLARAAASSAGSSDAAGLTRLPPGADGVLTMSAAERYVIEKALDRFDGNKTRA
                     AEALGISRQTLRQKMKDYGLAE"
     gene            complement(7887..>8795)
                     /locus_tag="VF720_11445"
     CDS             complement(7887..>8795)
                     /locus_tag="VF720_11445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880018.1"
                     /translation="RVVPISLRVTRLASEDGEADSARRPGGVVAIFDDLTDLKRSEAE
                     LRRKERLASLGELAAGVAHEIRNPLAGIGAAAQLLKKRLGEGDDKHPLAEIILTEAAR
                     LDRIVESLLRFGRPAPPRLAPGSVLDCVRRVMALVEETAGEAGVSLDIRLDPTIPDLY
                     LDQDQVVQVFLNLVQNALQAMPDGGTLSVSVARTEKSPWVRRTAGRRANDRAHLPPAA
                     PGAGYVQIRIGDTGHGIPKETLERIFDPFFTTRREGTGLGLSICQAILREHAGVIAVE
                     SVVGKGTTMIVTLPLEKRRGVRRRPG"
BASE COUNT         1327 a         3013 c         2957 g         1498 t
ORIGIN      
        1 ccaacgcctg gacgatgccg atcaaggccc gcatcgacat gctctccacc ggtgtgcgga
       61 cgccggtggg gatcaaggtg ttcggatcgg acctcgccac tatcgagacg attgccacgc
      121 ggatcgaggc ggtggtgcgc gaggtgccgg gggcgcggag cgtcttcgcc gagcgggcgg
      181 ccggcggctg gttcctcgac atcgagcccg atcgcgaggc gctggcccgc cacggcctca
      241 cgctggaatc gctgcaggaa accatcgcct cggcggtagg gggcgagaac gtcaccaccg
      301 tgctcgacgg gcgggcccgc taccccgtca acatccgcta cccccgcgag cagcgccagg
      361 acccggatgc gatcggccgg atcctggtgg ccacgggcca cggcgcccag gtgccgctct
      421 cgcaactggc ccgcgtggcg gtggtgagcg gtccgtcgat gatccgcaac gaatcgggat
      481 tcctggccgg ctacgtctac gtggacgtgg cggaccgcga tgtgggcggc ttcgtgcacg
      541 aggcgcgcga gaaggtgcgc aaggaagtgg agatgccggc cggctactcg ctcaagtgga
      601 gcggccagta cgagaacatg cagcgcgtcc aggaccggct gcggctggtc gtgccgatca
      661 cgctgcttct catcgccctg ctcctctatt tcaacacgcg gtccatgttc aagaccgtac
      721 tcatcctggt ggccgtgccg ttctcggcca tcggggccat ctggctcttc cactttctcg
      781 actacgacgt gtcgatcgcc gcctgggtgg ggatgatcgc cctgctcggc ctcgacgccg
      841 agacggccgt cttcatgctc ctcttcctgg acctgtcgtg gaaggaggcc agggaacgcg
      901 gcgcgctcaa ttccatcgcc gaggtggacg aggccatccg ccacggcgcc gtggcccgcg
      961 tccgccccaa gatgatgacg gtggcggccg cgttcatggg cctgttgccc atcctctggt
     1021 ccacgtcggc cggcgccgac gtgatgaagc ggatcgccgc cccgatgatc ggcggcctgg
     1081 tcacatcgtt cgccgccgaa ctcctccttt atccggccgt ctacaagcta tggaaacttc
     1141 gaaccgagtg ccggcatctc cggtactctc cgggtcgttc gcgcgacccc atctcaagcg
     1201 aaaggattac ccgatgacgc tccgcctccc tctcccgttc caaccggctc accggaccct
     1261 gatcctcggt gccgccacac tgttggttct cggtgccccg tgggcgttcc gcaccgacgc
     1321 gcgcgcggcc gacaagccgg ccggggtggt ggcgggctcc gacaaggcca ccgccggcga
     1381 cgtggccagc gcgccctatt tcctccccac cgatcccgtc accggtgaga agttgccggc
     1441 caagccggtg gtggccacga tcggggggcg ggagttccga tttgccagcg aggccaaccg
     1501 gaagacgttc gaggccaccc ccgacaaata cgtttccaag gtcgacaagg ccatgatcga
     1561 ggccgagaag cccttctacc cgaccaggga gtgcctcgtc tcgaacgagg aactcgggtc
     1621 gatgggggaa ccgatcgacc ggatcgtcgg caatcggctg gtccgcctct gctgcaagag
     1681 ctgcgtcgac aagctcgaca agaacccggc cacggtggtc aaggccctcg acgccgccac
     1741 cgtgaaggcc cagaaggcgg cctatcccct gaagtcgtgc gtcgtgtcgg gcgagccgct
     1801 cggggcgatg ggcgacccga tcgacatggt ggtgggcgga cgtctggtgc ggctctgctg
     1861 caagggatgc gtcgacaagc tcaatgccga cccggtggcg ttcctggcca gggtgccggc
     1921 cgcaggaaag aagtcgtagc ggtctgatcc cgcggttgcc gcgggacatg agtggccgag
     1981 cgaagcgacg gcgggtccgt tccggaacgg gcccgccgtt tcctttcccc ggagccgacc
     2041 gcaaccggcc gggttccgcg ctcagtcgat gacgcgcgcc atcgctttcc cgaacgtgtg
     2101 atggcccgac ttgtcgtaga ggtggatctc gacgggccac tccggggccg cgcagatctg
     2161 gtcgaacaac gcctcgtgtc cggtccgcgt gatgacgatg ccgaagctct cctcaccgcc
     2221 ggactcggag atgtcgtcca tcacctcgaa ctcgacgtcc cccttgcagt cggactggcc
     2281 gtacacgacg agtcgctggt cgacgtcctc gagatcggcg ctcccctgcg ggtcggtgac
     2341 agtggcgaag agattgagga agttgccacc ctggcattcg agggccacgt cggtcagcac
     2401 cggtccgtcc gcgtggtcgg aaatcggcag ggcctcggtg ccgcagcggc tccccccgtc
     2461 gtcgtcgtcc tcgtcgtcgc cgcaggaaga ggtcgcgacg accaccagcg cgaggccgag
     2521 aaccgccagc agggccgtga acctgcgggg acggccagcg agggaaaccg ggctggcggg
     2581 gtcggtggga ccggaggcga tcgtcatggg agtctcctcg atgactgtca gtcaccggca
     2641 gcgggaacga gatcgtgcgg ccgggcgggt tgcagtatac ccgggcgtcg gccgaccgga
     2701 ggaacgtctc accaggacgc gacgaggcgc cgaaggctgg cggatcgctg cccgatcccc
     2761 ttcggcgcca cgtcgagatg ctgcgtcact cccgggccac gggcccgggc acgcccggat
     2821 gtctgcctag atgttccctt ccgattccca cgcgtcgcga ccggcgtcca ccgcgtcacg
     2881 gacatcccgc ttcaggtggc tggtgacatc cttcaccttg tcgccgacct ggctcatccc
     2941 ttctttcacc cggccgccca tgtcgtccag ccggttcctg gcctggccac ccacgcggcg
     3001 ggccgcgtct ccgaggcggc ggcgcgtttc ggttccggtg gcgggggcca ggaggagtgc
     3061 catcccggcg cccagcaccg ctcccacgac gatccccagc atcacgccct cgctgttgct
     3121 tcgatactca cgcatagccg ttccctcctt cggtgatcga atagttcttg aggcgattcc
     3181 gcccccagcg gcttgccagc acgctgccgg ccgccttgat cccgcgcacg agggcggtca
     3241 ccttgcgggc cggcccctcc acttcgtcga gcagggcacc cgagattcgg gccccccgtt
     3301 cgctgactcc cttgaagtcc ccgacgatgc cgtccacgcg atcgaccacg tcgtcgagcg
     3361 aaaccaggag ctcccgaagt tcggccgacg cccgggtggc atccgtgatg agcccttcca
     3421 ggggcagtag cgaacgatcg acgttgtcga gcgtgtgatt cagccgccgc atcgtgacga
     3481 cggccacgac aatcaggatg atcagtccgg ccgtgagtgc cacgatgcac ccctggatca
     3541 acggcgtcat gttctgcccc tttctcggtt cgagagcacg tatccggtct gtccgacgcg
     3601 ggtttgtgca aggggcgttc cagcgccacg gatggtcggg gccagttcgc aagtcacacg
     3661 aacgggtggg atcaagtcat gaaaacaagg ccacttgcag ggccaccttc aagcaggccc
     3721 tgaagggttg gcgcggggca tgcgcaagcg ccggccagcg gcgcttggtc gaaattaccg
     3781 tcgggcatgt cccgctgtcg gagcggcaat cggacgtcgg aaaatttgtc gcccggcggt
     3841 cagccgcgtg tcgatccctt gccgatcggc ggcacctcga tcccgagggc ggtggccagg
     3901 tcgatggcat gctcctgttc ctggaccagg atctgccgga gctcctcggc cagcgcgtac
     3961 tcctccagtg cttcggcctg gcggacgcgc tcccggtagt tccggatcgt ctcggtctcg
     4021 ttctccaggt cgaaccggag gagcgccttc ggctcgtcgg agatcttgac cggcttcggg
     4081 acggtgatcg gcgtgccgcc gaggtagtcg atctgcctgg ccacccggat ggcgtgctgc
     4141 agctcctcgc cggcatgaat ttccagttcg ccggcaatgg ccatgtactc ggcccccttg
     4201 agcacctgcg aatagacgac gtaagcgatg atcgcctgat actcgcggct gaggtcgtcg
     4261 ttgagcgcgt cgatcaggac ctggcgctcg ctccgcgccg agggtcgaac gcccttcgtt
     4321 cgttcgttgg caggcatgta agcctcctcg tgtctggtat ggtcgatgac gttcgtcccg
     4381 gcgcggccct gattcacgat gacacagtcg accggggagc gtccctccgg aaatccccca
     4441 cccgaggaat ggcagtgcgt ggaatcgaca tcccgctccc ccgctggctc tatcacgtgg
     4501 ggatcgcgct tcgcggcacg atgaggctct gggtgtccca tcgcgcggac cgactggcgg
     4561 cggccctggc cttctacgcc gtcttctcga tggcgccgct cctggtcatc gtgattgcgg
     4621 tagcaggcca ggtgttcggg cccgaggcgg tccgcggcga aatcgtcgcc cagttcggct
     4681 cgctgatcgg cgtcgaggcg gcccggcagg tggaggtgat catccagggg gccagcaatc
     4741 cggcctccgg gctgatcgcc gcgctgatcg gcggcctttc gctcatggcc ggcgccacgg
     4801 cggtcttcgc gcaactgaag gaagcgctga acgccatctg ggacgcgccg ccgctcgagc
     4861 gcggtttcct gaagaccgtc ttccgcgacc ggatccgctc actggcgatg gtcttcggcg
     4921 tggcgttcct gctgctggtt tcgctggtgc tctcggccgc cctggcggcg ctcacccggt
     4981 gggcggcccc ctaccttccc cacttcccct tctcgctcgc cggcaccatc gacttcgtgc
     5041 tgtcgttcat cctggtgagc gtgttgttcg cgatgatcta ccggattctt cccgacgtcg
     5101 cgatctcgtg gggcgacgtg gcgatcgggg cggccgtcac cgcggtgctc ttcaacctcg
     5161 gcaagtacct gatcggcctc tacctcggtc agagcacgct gggatcggtg ttcggcgcgg
     5221 ccggatcgct cgcgattctc atgttgtggg tgtactactc ggcggcggtc ttcctgctgg
     5281 gcgccggatt cacgcgggtc tactcgttga cctggggaag cctgtcccgg aaggcgagcg
     5341 gcacgagcgt gacgaccgtg gaaggtccgg cgtcgttgtt gtccgccagg aggtagaggg
     5401 tgccgtccgg gccgaaggca atcccttcgg ccgtgccgaa tggcgagagg ggcgagtcgg
     5461 gatcgcgctt gttctcaagc gtcgactcga tcgcgtcgaa gtcgagccac tcccgcaccg
     5521 aaacccggtt ctcgctcgag tcgcgcacgg ccgcctcgat ccccttccga tcgaccacgg
     5581 cgatgcggcg ggccagccgg tcgagcacgt agagatcgcc cgagggaccg aagcaggcgc
     5641 ccgcctgggt aagcagccca tcgatccggt ccatgcggat cggctcgatc ggcgtgagcg
     5701 acaccgagtc ggtgcccgcc gccggagccg ccacgatcgg gcccgccgtt ccctgctttt
     5761 ccgtgaagag gaaactccgg acgtaggtgg ggtcgggacg ttccttgaac gccagcacca
     5821 ccagcgtatc ggcgccgagg cgcatcatgg ccaccccttc gaacccgttg ttccccaccg
     5881 tctcccccgg gggggaacct cgcccggtcc agcggagggt gaggcgcccg tcccgctcct
     5941 cgaagagatg gagccacgac accgactcgc catccggccc ggcctcgctg gcgccggcca
     6001 cccacaccgg acgattgcag ggaccggtga gccgggccag gtcttccatg tcgtcgccga
     6061 aagggagccg ccgcggattg aggatggcca tcggcgagag gagcagcgtc cgcggcgaac
     6121 cctctccggc gggacgcgcc cggatgatca tcgactcgat cctgttggac ttgtcgtcgg
     6181 ccagccagaa ctcgaagtgg cggccccgct gcacgctgtc cacgcaggcg ggacccgtcg
     6241 gcaccacgat gccggaaaaa tcggtgcgcg gtttcgaagg cccggcggcc acccaggcca
     6301 cgcggaaacg gctttcgatc ggtacccgca gcgagtcggg gagctcggcc gcgccggagt
     6361 cggcgaagcg gccatgcggg cggtgcagga acactgccac gaggagggcg ctggtaatgg
     6421 ccacgaggag gcccaccacg gcggccgtgc gggaggaacc gggggacgcg gtcactcggc
     6481 cagcccgtag tccttcatct tctggcgcag ggtctggcgg ctgatgccga gcgcttcggc
     6541 cgcgcgcgtc ttgttgccgt cgaagcgatc aagggccttc tcgatcacgt atcgctccgc
     6601 ggcggacatg gtgagcactc catcggcccc gggggggagg cgcgtgagcc cggcggcgtc
     6661 gctcgaaccg gcgctcgagg cggcggcccg cgccagcagc tcggacggca ggtgctcggc
     6721 ggcaatggcg ctggcgtcgt ggaggaggag cgcccgttcg atcacgttct tgagctcgcg
     6781 cacgttcccc ggccagggat aggccagcat cagcgccatc gcccggtcgt ccacaccggt
     6841 caccgatcgg ccgagctcgc ggttgaagtg cttgatgaag tgcgccacga gctgcgggat
     6901 gtcgtcctgc cgttcgcgca gtggtggaat ctgcaccggc acgaccgaca ggcgatagta
     6961 gagatcctcg cggaagcctc cgtcggccac cgatttcttc aggtcccggt gggtggcggc
     7021 cacgaaccgg acgttgacga ccacatcggc cacgcctccc acccgcttga acttgcgggt
     7081 ctcgaggacg cgcaggagtt tgggttgcag cgactgcggc atctcgccga tctcatcgag
     7141 aaagagggtg ccgccatccg ccaggtcgag gagccccttc tttcgaccct tggcatcggt
     7201 gaaggccccc ttctcgtggc cgaacagctc ggtctcgatc aggttctcgg gaaacgagga
     7261 gcagttcacc tcgacgaact ggccggcggc caccgggctc ttctcgtgga tacgccgcgc
     7321 cagcagttcc ttccccacgc cggtctcccc caggaggaga gccgtggtgg ccgcgccggc
     7381 gatccggtcg atctcgtcca cggcccgctt catggccggg ctcttccctt ccacgagctc
     7441 cagatggtga cgcccgctct ccgtcctttg caagaattcc acctggcgac ggagcgtgcg
     7501 gttctcgagc gccgcccgaa ggacgaggac cagctggtcg atctcgaacg gcttgccgag
     7561 atactggtag caaccgagct tggtggcctc caccgccgac tcgaaccggc caaaggcggt
     7621 catcatcacc acttccatgt cgggccactc cttcttgatg gccttcagga tctcgatgcc
     7681 gtcctcgccc gactccttga gcttctgatc gagaaggacg aggtcgatct cctcctcgcg
     7741 cagctgcatg aggccgctct cgccgccggg cgcctcgaac acgcggaacc cttcgccgcg
     7801 caacgcctcg ccgatggtga accggagcga acgctcgtcg tcgatgatca ggacgccggg
     7861 gccggacacc ggaagtcgtt cgtcggtcat ccaggcctcc gccgtacgcc gcgccgcttt
     7921 tcgaggggaa gcgtcacgat catggttgtt cccttgccga cgaccgattc gacggcgatg
     7981 acgccggcgt gctcgcgcag gatggcctgg cagatggaaa ggccgaggcc ggtcccttca
     8041 cggcgcgtgg tgaagaaggg atcgaagatc cgctccagcg tttccttcgg gatgccgtga
     8101 ccggtgtcgc cgatccggat ctggacgtac cccgcgcccg gcgccgccgg cggcaggtgg
     8161 gcacgatcgt tggcgcgacg gccggcggtg cggcgcaccc agggtgactt ctcggtgcga
     8221 gccaccgaca ccgacagggt gccgccatcg ggcatcgcct ggagcgcgtt ctggacgaga
     8281 ttcaggaaca cctgcaccac ctggtcctgg tcgaggtaaa gatcggggat cgtcggatcc
     8341 aggcggatgt cgagcgagac gccggcctcg ccggccgtct cctcgaccag tgccatcacg
     8401 cgtcgcacgc aatcgagaac cgaccccgga gccagtcggg ggggcgccgg gcgcccgaat
     8461 cggaggaggc tctccacgat tcgatcgagc cgcgccgctt cggtcaggat gatttccgcc
     8521 agcggatgct tgtcgtcccc ctcccccagt cgtttcttga gaagctgggc cgccgcgccg
     8581 atcccggcca gcgggttcct gatctcgtgg gccacgccgg ccgcgagttc gcccaggctg
     8641 gccaggcgct ccttccgccg gagctcggct tccgagcgct tgagatcggt gaggtcgtcg
     8701 aagatggcca ccacgccgcc agggcgacgt gcgctgtcgg cctcgccatc ctcggatgcc
     8761 agcctcgtca cgcggagcga gatgggcacg acgcg
//
LOCUS       DASVOD010000513         8277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_973825, whole genome shotgun sequence.
ACCESSION   DASVOD010000513 DASVOD010000000
VERSION     DASVOD010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_973825"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF720_11450"
     CDS             <1..280
                     /locus_tag="VF720_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880019.1"
                     /translation="YHQREGKSKIHPIRDTIGFTNLIVRTTLYFNPLKIFMPLSGLVL
                     LAAVGLLVYSKFVLGRVMDISVIVLLSAALQIAVIGMLADLIDKRGHR"
     gene            277..1725
                     /locus_tag="VF720_11455"
     CDS             277..1725
                     /locus_tag="VF720_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7880020.1"
                     /translation="MRALLVNPPDLHTVVANNPTVIDEERGHHPPLGILHVAGYLRAF
                     TEHQVAICDAQVEKLDFAAIRQRIIDFQPDLVGITAMTFTILDVMEICRIAKSVNPDI
                     VVALGGPHVHIFQTETIRLPFVDVIVIGEGETTFADLMNNLRDPAGMRKVAGIVYKDK
                     DGSMVNTGNRPLLDDLDILPFPARDLTPYTKYTHLMAKHQPITTMFTSRGCPYKCTFC
                     DRPHLGKNFRARSPMSVVDEMEEIVNLGIRETLIYDDTFTINKRRVHEICDEILARGI
                     KLGWDIRARVNTVDEELLAKMKAAGCERIHYGVEVGNDRMMKVLKKGLTISQVERGFK
                     MTKAAGIETLAYFMIGNPTETREDIMDSVALAKRIDPDYIHLTILTPFPATEVYFEGL
                     KQGILKKDFWREFAKNPSADFEPEYWLENFKKEELFELLVHSYKAFYQRPSYVFKVGS
                     RVRSVGEFMRKASAGLKIFGMNARGATSLREI"
     gene            1715..2692
                     /locus_tag="VF720_11460"
     CDS             1715..2692
                     /locus_tag="VF720_11460"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7880021.1"
                     /translation="MKSDPRLAWLVRAIGVGLFVILIARSDPGAIAGVLRRANPATIG
                     LAGLLIFPMIAFKTARWRDLQRAVRLPMAPFGESFRAYLEGLFAGLVTPGRVGEFVRV
                     RSLTRQGAAFGPAMATVLWDRVFDVLGLFLLGSLALVPLAGTFRGLYVGSLAALGGGL
                     LVTAFLVWGPTGGWRRFMTPRTPTQGHSGQGLAGKLRESLAGLLEASRSFRGGLLVRV
                     GLLTLLGWVVYYAQAWLLARAIHLDLRLDALIVAVTAAAVAAFLPISISGIGTRDAAL
                     VVLFTRLGETREEALAYSALVLAMLLLNALVGLVASRWPVGEMARRVSD"
     gene            3198..3851
                     /locus_tag="VF720_11465"
     CDS             3198..3851
                     /locus_tag="VF720_11465"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7880022.1"
                     /translation="MARPAIVAADFTVDDSGFYKRYGKRLLDILGSSGLLLLLLPVFP
                     VIALAIALSSRGPVLYRSTRLGKGGKPFTFLKFRSMVVDAHENRHSLLHLNEVDGPVF
                     KLANDPRVTQVGGFIRRTSIDELPQLLNVLRGEMSLVGPRPPIPDEVEKYEPWQRRRL
                     DVTPGITCLWQISGRSKLGFNEWMRLDLQYIQHRSFKLDMKILLRTLPAVLSRDGAY"
     gene            3892..4968
                     /locus_tag="VF720_11470"
     CDS             3892..4968
                     /locus_tag="VF720_11470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880023.1"
                     /translation="MIPIALVSDARYEGGAERYLLRLAGALDRRRFRPTLVMPDREGL
                     DDLIERGRRAGLDVVRWRRRPEPLAAIELARAVDRLAPRIVHLNMPSPYEMGCGGWAL
                     GLSRPERSVVATEHIADIAPSRRRVLQKRLWSGLIERTITISAAHAALLASRHGVPKE
                     KIRVVMNGVEDPGPSPERPDRPLRVAVIGSLEPRKGQDRLLEAAAMALGRGADIEVVL
                     VGAGPSRSALEAAAARAPLSGRVRFTGQLASAAREIASSHIVAVPSRIEGVPFVVAEA
                     MAAGTVPLVSRLPGLDEVVDDGVGRLLPADDASVWADALVELAANPAERARLAAAARA
                     RFEQRFTLARMAAGTAAVYAEVLR"
     gene            4965..6161
                     /locus_tag="VF720_11475"
     CDS             4965..6161
                     /locus_tag="VF720_11475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880024.1"
                     /translation="MRIAIVKPIIPWPAVQGTRRVTMGLIDALAGEHEVTLVAPSLDA
                     ADEVAADTLARERNIRVVTVRAPNRRSLFHRGWYRAAYAVAARLGGHSPRALYATPPA
                     LFDRLARESAGHPFDLAIFEYWYTYRAFHRVPSRHRVLLAHDADFAIAGRKDGASFAS
                     AESRREAEACRNVEEVWTLTTADREALAAHAGLPRDRFRIMPFGVDTTALDLEAGSND
                     GTVLFFGAWQADFNVDALGWLLDEIWPAVKRRLPTTRLEVAGGGMPEALAERVRKAGG
                     TVSGRVEDLAALYRRAAVVLIPLRFGGGLRIRLIEALAARRAVVSTAIGIGGLEGRDG
                     RDWLLASDAEGLAEATACVLRDPALGRRLGEAGRELVATRYSDASAAEGLRRLVTRFD
                     DRRATG"
     gene            6201..6671
                     /locus_tag="VF720_11480"
     CDS             6201..6671
                     /locus_tag="VF720_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7880025.1"
                     /translation="MLIRLPDFLESSHFIPELTASDRDGALREMVGVLAAASQVRDGE
                     ILLEMLRQREQFGSTGIGRGVAVPHGRSLAIARLMVIVGRSTAGIDFDSMDGKPVHLI
                     FLTVAPPQDRANLYLPVLGKIVEAVKSARIRKKLLAAPDFTAFMAVMAEADDDE"
     gene            6664..7017
                     /locus_tag="VF720_11485"
     CDS             6664..7017
                     /locus_tag="VF720_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX7880026.1"
                     /translation="MSDPVRQLVQLQDLDIMIREISDPERARELKTLGFEMTGLERLK
                     AARMHLAQSIEKRWLVVYERLARKHGRAVVPVEDKTCLGCFQGLPGSFYSEISADQPV
                     KLCENCGRILYLLNR"
     gene            7022..8200
                     /locus_tag="VF720_11490"
     CDS             7022..8200
                     /locus_tag="VF720_11490"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7880027.1"
                     /translation="MAGHFSHLECGRAGHVTPADRLVNLCHCGSPLLARYRLDDVRRE
                     LPREALKDRPPSLWRYRELLPAAPEEAVVTLGEGMTPLLSACRLGATAGCTDLWIKDE
                     SANPTGSFKARGMSVAITMARHRGARALSLPTAGNAGSAAAAYGARAGLPVHVFMPAD
                     TPPLFLAECRSLGAHVIEVEGVITDAGRAMAETGKDLGWFDLSTLKEPYRLEGKKTMG
                     FELAEQFDWRLPDVILYPTGGGTGLVGMWKAFDEMEALGWIDSRRPRLYSIQAAGCAP
                     IVRAFEAGAGSAEPFPSPHTFASGLRVPRAVGDFLILRAVRETGGLAVAVDDEAIRRA
                     WREIGVAEGIFAAPEGAATWAGLGKLLAMGHVRPDERIVLFNTGTGLKYAEAWPGESA
                     "
BASE COUNT         1298 a         2860 c         2730 g         1389 t
ORIGIN      
        1 ctatcaccag cgcgaaggga agtcgaagat ccatccgatc cgggacacga tcgggttcac
       61 gaacctgatc gtccggacca cgctctattt caacccgctc aagatcttca tgcccttgag
      121 cggactggtg ctcctggcgg ccgtcggcct cctggtgtac agcaagttcg tactcggtcg
      181 cgtgatggac atctcggtca tcgtacttct atcggccgcg ctccagatcg ccgttatcgg
      241 gatgctggcg gacctgatcg acaagagagg acaccgatga gagcactgct ggtaaacccg
      301 cccgacctgc acaccgtcgt ggccaacaat cccacggtca tcgacgagga acgtggacac
      361 cacccgccgc ttggaattct ccacgtggcg ggttacctgc gcgccttcac cgagcaccag
      421 gtggccatct gcgacgcgca ggtggagaag ctcgacttcg cggcgatccg ccagcgcatc
      481 atcgacttcc agcccgacct ggtcggcatc accgccatga ccttcacgat cctcgacgtg
      541 atggagatct gccggatcgc caagtcggtg aatcccgaca tcgtggtggc cctcggcgga
      601 ccgcacgtgc acatcttcca gaccgaaacc atccgcctgc cgttcgtcga cgtcatcgtc
      661 atcggcgagg gggagacgac cttcgccgac ctgatgaaca acctgcgcga cccggccggg
      721 atgcgcaagg tggccggcat cgtctacaag gacaaggatg gctcgatggt caacacgggc
      781 aaccgcccgt tgcttgatga cctcgacatc ctgccgttcc cggcacgcga cctcacgccc
      841 tacacgaagt acacgcacct gatggccaag caccagccga tcaccaccat gttcaccagc
      901 cgcggctgcc cctacaagtg cacgttctgc gaccggcctc acctgggcaa gaacttccgc
      961 gcccgctcgc cgatgagcgt ggtcgatgag atggaggaga tcgtcaacct cggcattcgc
     1021 gagacgctga tctacgacga caccttcacg atcaacaagc gtcgcgtgca cgaaatctgc
     1081 gacgagatcc tggcccgcgg aatcaagctc ggctgggata tccgcgcccg cgtcaacacc
     1141 gtggacgagg aactgctggc caagatgaag gcggccggct gcgagcgcat ccactacggc
     1201 gtggaggtcg gcaacgaccg gatgatgaag gtcctcaaga agggactcac catcagccag
     1261 gtcgagcgcg gcttcaagat gacgaaggcc gccggcatcg agacgctggc ctacttcatg
     1321 atcggcaacc ccaccgaaac gcgcgaagac atcatggact cggtggcgct ggccaagcgc
     1381 atcgaccccg actacatcca cctgacgatc ctgaccccgt tcccggccac cgaggtctac
     1441 ttcgaggggc tgaagcaggg gatcctcaag aaggatttct ggcgcgagtt cgccaagaac
     1501 ccgagcgccg acttcgagcc ggaatactgg ctggagaact tcaagaagga agagctcttc
     1561 gagctgctcg tgcactcgta caaggcgttc taccagcgcc cgtcgtacgt gttcaaggtc
     1621 ggcagtcgcg tccggtcggt cggcgagttc atgcgcaagg catccgccgg cctcaagatc
     1681 ttcgggatga acgcgcgagg ggccacttcg ctgcgtgaaa tctgaccccc ggctggcctg
     1741 gctcgtccgg gccatcggcg tcggactctt cgtcatcctg atcgcgcgga gcgatcccgg
     1801 cgccatcgcc ggggtgcttc gccgggccaa cccggccacg atcggattgg ccgggctcct
     1861 gatttttccg atgatcgcat tcaaaacggc ccgctggagg gatctccagc gggccgttcg
     1921 tcttcccatg gccccgttcg gggaatcatt ccgcgcctac ctcgagggcc tgttcgccgg
     1981 gctcgtcacg ccgggccggg taggggagtt cgttcgggtc cggtcgctga cccggcaggg
     2041 cgccgctttc ggccccgcca tggccaccgt gctctgggac cgggtcttcg acgtcctcgg
     2101 gctcttcctc ctcggcagcc tggccctcgt cccgctggcc ggcacgtttc gcgggttgta
     2161 tgtcgggagc ctcgccgccc tgggaggcgg tctcctcgtc actgccttcc tggtctgggg
     2221 gcccaccggg ggctggcgtc ggttcatgac tcccagaacg cccacccagg gacactccgg
     2281 ccaggggctg gccggcaagc tccgggaatc gctggccggg ctgctggagg cctcacgttc
     2341 cttccgcggg gggctcctcg tccgggtcgg cctcctcacc ctgctcgggt gggtggtcta
     2401 ctacgcgcag gcctggctcc tggcccgggc catccacctc gacctccgtc tcgatgccct
     2461 gatcgtggcg gtgacggcgg ccgccgtggc cgccttcctc cccatttcca tctcggggat
     2521 cgggacgcgt gacgccgccc tggtggttct tttcacccgc ctgggggaga cccgcgaaga
     2581 agcgctggcc tactcggccc tcgtcctcgc catgctcctc ctgaacgccc tggtcggcct
     2641 cgtggcctcc cgctggcccg tgggggagat ggcccgccgc gtttccgatt gactcatccg
     2701 gaagcctcat gatatgttcg ctgtccaatc acaagcgcct caccaactta cggagggctc
     2761 gccagcgacc actttctaac aggtacgagc aggaaatcca ccttcgaatc ccagactgaa
     2821 gctggagtca tcaatcccga tagcaaccta gcctagcgac cccggaccgg cgctgtttgc
     2881 ctgtgcctcc aaccggggat gatgaacgag tcgggtgacg gacaactgga gcgggcaacc
     2941 acttcggccg atcaccatgg gaacggttcg aatgctggag acaagcaggg gagctgagat
     3001 ccaccgctga gacggttgat gctctccttt ttttgtcaac agatgccgct gactacaacc
     3061 tgagctgcct catgttcaag acggcaaaca gagaaccgcg agaagggatc ggccccgtca
     3121 tgaagagttc ttcattggca tccaacctgg caacggcccc cactcccgtt cgcggacagg
     3181 ccaatcgagc ggtcagcatg gcccgacccg cgatcgtcgc cgccgacttc acggtggatg
     3241 acagcgggtt ctacaagagg tacggaaaac gtctcctcga catactgggc tcgtccggcc
     3301 tgctcctcct tctcctccca gtcttcccgg tcatcgcgct cgccatcgct ctctcctccc
     3361 gcgggcccgt cctctaccgc tccacacgcc tcggcaaggg cggcaagccc ttcaccttcc
     3421 tcaagtttcg atcgatggtc gtggatgccc acgagaaccg gcattcgctg cttcacctga
     3481 acgaagtgga cggcccggtc ttcaagctgg ccaacgaccc ccgtgtcacc caggtgggcg
     3541 gctttatccg ccgcaccagc atcgacgagc tgccgcagct cctgaatgtc ctgcggggcg
     3601 agatgagcct ggtcggcccc cggccgccga ttccagacga ggtggagaag tacgagccgt
     3661 ggcaacgccg gcgcctggac gtcaccccgg ggattacctg cctgtggcag atcagcgggc
     3721 ggagcaagct cggcttcaac gagtggatgc ggctggatct ccagtacatc cagcatcgct
     3781 cgttcaagct cgacatgaag atccttctcc gcacgctccc ggccgtcctc tcccgcgacg
     3841 gcgcctactg acaatcggac ggcccggcgc gctgccgggg cgtcttcccc gatgatcccg
     3901 atcgcgttgg tttccgacgc ccgctacgag gggggcgcgg aacgctacct gctgcgcctg
     3961 gccggcgcgc tcgaccggcg gcgctttcga cccacgctcg tgatgcccga tcgcgagggg
     4021 ctggatgacc tgatcgagcg gggtcgccgc gccgggctcg atgtcgtgcg ctggcgccgc
     4081 cgcccggagc ctctggcggc catcgagctg gccagggccg tcgatcgact cgcgccccgg
     4141 atcgtgcacc tcaacatgcc gagcccgtac gagatggggt gcggtggctg ggctctcggg
     4201 ctgtcgcgcc cggaacggtc ggtggtggcc acggagcaca tcgccgacat cgcgccgtcg
     4261 cggagacggg tgttgcagaa gcgcctctgg tcgggcctga tcgagcggac cattaccatt
     4321 tcggccgcgc atgccgcgct cctcgcgtcg cgacacggcg taccgaagga gaagatccgg
     4381 gtggtcatga acggggtcga ggatcccggc ccctcgcccg aacgtccgga tcgcccgttg
     4441 cgcgtggccg ttatcgggag tctcgaacct cgcaaggggc aggaccgtct cctcgaagcc
     4501 gcggcaatgg cccttggccg gggcgccgac atcgaagtcg tcctggtcgg cgctggtccc
     4561 tcacggtccg cgctcgaggc ggcagccgca cgtgcgccac tgtccggacg cgttcgtttc
     4621 accgggcagc tcgccagcgc ggcccgcgag atcgcgtcga gccacatcgt ggccgtgccg
     4681 tcccggatcg aaggagtgcc gttcgtggtg gccgaggcca tggccgccgg taccgtgccg
     4741 ctcgtctccc ggctccccgg gctcgacgag gtggtggacg atggcgtcgg tcgcctgctc
     4801 ccggccgacg acgcgtcggt ctgggccgat gcgctggtgg agctggccgc aaatcccgcc
     4861 gaacgtgcgc gcctggccgc ggcggcacgg gcgcgtttcg aacaacgctt cacgctggcc
     4921 cgcatggcgg ccggcacggc ggccgtgtac gccgaggtgc tccggtgagg atcgcgatcg
     4981 tgaagccgat catcccgtgg ccggcggtgc agggaacgcg ccgggtgacg atggggctca
     5041 tcgatgcgct cgccggggag cacgaggtga cgctggtcgc gccgtccctg gatgccgccg
     5101 acgaagtggc ggccgatacg ctcgcgcggg aacgaaacat ccgcgtggtg accgtccgtg
     5161 cgccgaaccg tcgctcgctg ttccatcggg gctggtaccg ggcggcctac gccgtggcgg
     5221 cgcggctggg cggccactcg ccacgggccc tttatgccac gcctcccgcg ctgttcgacc
     5281 ggctggcccg ggaatccgcc ggacacccct tcgacctggc gatattcgag tactggtaca
     5341 cctaccgggc cttccaccgg gtgccgtcac ggcaccgtgt gctcctggcg catgacgccg
     5401 acttcgccat cgccggccgg aaggatggcg cctcgttcgc ctcggccgag agccgccgcg
     5461 aggccgaggc gtgccggaac gtggaggagg tctggaccct gacgactgcc gatcgtgaag
     5521 cgctggcggc gcacgccgga cttccccgcg atcgtttccg gatcatgccg ttcggtgtcg
     5581 acacgacggc gctcgacctc gaggccgggt ccaacgacgg gacggtcctg ttcttcggcg
     5641 cctggcaggc cgatttcaac gtggacgcgc tcggctggct gctcgacgag atctggcccg
     5701 cggtcaaacg tcgtctgccg acgacgcggc ttgaagtggc gggaggcggc atgccggagg
     5761 cgctggccga gcgcgtgcgg aaagccggcg gcacggtgag cggtcgggtg gaggatctcg
     5821 cggccctgta tcggcgggcc gccgttgtgc tcatcccgct ccgcttcggt ggcgggctgc
     5881 ggatccgcct gatcgaggcc cttgccgcgc gtcgggccgt cgtcagcacc gcgatcggca
     5941 tcggtggact ggaaggaagg gatgggcgcg actggctcct ggcctccgac gccgaggggc
     6001 tggcggaagc cacggcttgc gtactgcgcg atccggcact gggccgtcgg ctcggcgaag
     6061 ccggccgtga actcgtcgcg acgcggtatt cggacgcctc ggccgcggaa gggctccgcc
     6121 ggctcgtcac gcgattcgat gaccgccgag ccaccggctg accgcccgcg atcgcccgaa
     6181 ttgctttata cttccgccct gtgctgatcc gactccccga cttcctcgaa agctcccact
     6241 tcatccccga attgaccgcc tccgatcggg acggcgcgct tcgggagatg gtcggtgtcc
     6301 tggccgccgc gagccaggta cgcgacggcg agatcctcct ggaaatgctg cggcagcgcg
     6361 agcagttcgg ctccaccggc atcggacgcg gggtggcggt gccgcacggg cgatcgctgg
     6421 ccatcgcccg cctgatggtg atcgtcggcc ggtcgaccgc cggaatcgac ttcgactcga
     6481 tggacggcaa gccggtccat ctcatcttcc tgacggtggc gcctccgcaa gacagggcca
     6541 atctctacct gccggtgctg gggaagatcg tggaagcggt gaagagcgcc cgcatccgca
     6601 agaagctcct ggccgcaccc gatttcacgg cattcatggc cgtcatggcc gaggcggacg
     6661 acgatgagtg atccggtgcg ccagctggtc caactacaag atctggacat catgatccgc
     6721 gagatctccg atccggagcg ggcccgcgag ctgaagacgc tcggcttcga gatgaccggg
     6781 ctcgaacggc tcaaggccgc ccggatgcac ctggcccagt cgatcgagaa gcgctggctc
     6841 gtggtgtacg aacgcctcgc caggaagcac ggccgcgccg tcgtgccggt cgaggacaag
     6901 acctgcctgg gctgcttcca gggacttccg ggttcgttct actcggagat cagcgccgac
     6961 cagcccgtca agctctgcga aaactgcggg cgaattcttt atctcctgaa ccggtaatcc
     7021 cgtggccggt cacttctccc acctggagtg cggccgcgcg gggcatgtca cgcccgccga
     7081 tcggctcgtc aatctctgtc actgcggatc gccgctcctg gcacgctatc gactggacga
     7141 cgttcgccgg gagctgccgc gcgaggcgct gaaagaccgg ccgccttcgc tctggcgcta
     7201 ccgggagctt ctcccggccg ccccggagga agcggtcgtc acgctcggcg aggggatgac
     7261 cccactcctg tcggcatgcc ggctcggcgc gaccgccggt tgcaccgacc tctggatcaa
     7321 ggacgaatcg gccaacccga ccggctcctt caaggcacgc gggatgagcg tggccatcac
     7381 gatggcgcgg caccgtggcg cacgggcgct ctcgttgccg acggcgggaa atgccgggag
     7441 cgccgccgcg gcctacggcg cccgcgcggg ccttcccgtg catgttttca tgccggccga
     7501 cacgccgcct ctcttccttg ccgaatgccg ctcgctcggc gcccacgtca tcgaagtgga
     7561 gggcgtgatc accgatgccg ggcgcgccat ggccgagacg gggaaggacc tgggatggtt
     7621 cgacctttcc accctcaagg agccgtaccg cctggaaggg aagaagacga tggggttcga
     7681 gctggccgag cagttcgact ggcggctccc cgacgtgatc ctctacccga ccggcggcgg
     7741 taccgggctc gtcggcatgt ggaaggcctt cgacgagatg gaggcgcttg gctggatcga
     7801 cagccgccgg ccgcgcctct actccatcca ggcagccggg tgcgccccga tcgtgcgggc
     7861 cttcgaagcg ggcgccgggt cggccgaacc gtttccgtcg ccgcacacat tcgccagcgg
     7921 gttgcgcgtg ccgcgggcgg tgggcgactt tctcatcctg cgcgccgtgc gcgagacggg
     7981 cggcctggcg gtggcggtgg acgacgaggc catccggcga gcctggcgcg aaatcggcgt
     8041 ggcggagggg atcttcgcgg cgccggaagg ggcggccacc tgggcgggcc tcggaaaact
     8101 gctggcgatg ggacatgtgc ggcccgacga gcgcatcgtg ctgttcaaca ccggcaccgg
     8161 gctcaagtac gccgaggcct ggccgggaga atcggcttga accgccccga acccccgccc
     8221 gccggcgcgg tgccgttccg gctgcgccgc ttcctccagc gcctccacga aatcgac
//
LOCUS       DASVOD010000514         1647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_87014, whole genome shotgun sequence.
ACCESSION   DASVOD010000514 DASVOD010000000
VERSION     DASVOD010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87014"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VF720_11495"
     CDS             <1..516
                     /locus_tag="VF720_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880028.1"
                     /translation="ATFGTKPRTVADHDWAWWRNLAREQQTRGNARNAALLYNMAMDL
                     LVPAPWVRPDMLQQLMAEQRKLHPENLPNNRKERWVTTDDTEFFPFQASCDIVPGGVG
                     VRFTYEVEEGADSTAVKAAAPKLVDFVRTTFPEYAEVFRVVRLEAVSAGNRELVWVGE
                     FPLKPAPGPPQ"
     gene            518..>1647
                     /locus_tag="VF720_11500"
     CDS             518..>1647
                     /locus_tag="VF720_11500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880029.1"
                     /translation="MSRSTNAPARADGRLTLAYLSIGRHIHTERWLSWFARRGHQVHL
                     LTVQPGPIPGVTVHDITTHHGPKPLRYLKSLGRVKEILKVLDPDLLHTHFLTGYGYWG
                     HYSGRKPHMLTVWGDDVYVTPFESPLKNWLARQVLRGADYLTGDSADIIEACIKLGAR
                     RETSEVVQWGVDFTRFHPDAAGPVRDALGIPTEAPVVLSTRSFTQAYYNIDVIIDSIP
                     LVQKTFPEVIYIIAGNEGSDEAIRARARERGVEAACRFVGRIPHADLPSFLTSSHVYL
                     TVPSVDATAVSLLEAMATGTAIIASDLPSATEWIRDGETGRVVKPREVEGLARVTVEL
                     LADGGLRRRLGERAVSLVRDGADHDRNMARVEAIYRELVAKK"
BASE COUNT          272 a          566 c          562 g          247 t
ORIGIN      
        1 gccacgttcg gcaccaagcc gcgcacggtg gccgatcacg attgggcctg gtggcggaac
       61 ctggcccggg agcagcagac gcgcggcaat gcccgcaacg cggcgctcct ctacaacatg
      121 gccatggacc tcctcgtgcc ggccccgtgg gtgcggccgg acatgctgca gcagctcatg
      181 gccgagcagc ggaagctcca cccggaaaac ctgcctaaca atcgcaagga gcgctgggtg
      241 acgacggacg acacggagtt cttcccgttc caggcttcgt gcgacatcgt gcccggcggc
      301 gtgggggtgc gcttcaccta cgaagtggag gagggcgccg attcgacggc cgtgaaggcc
      361 gcggcgccga aactcgtcga cttcgtgcgg acaaccttcc cggagtacgc cgaagtgttc
      421 cgggtcgtgc gcctcgaggc cgtgtcggca ggcaaccggg aactggtgtg ggtcggcgaa
      481 ttcccgctga agccggcgcc ggggccgccg cagtagcgtg agccgttcca ccaacgcccc
      541 ggcccgggcc gacgggcggc tcacgcttgc ctacctctcc atcggccggc atatccacac
      601 cgagcgctgg ctcagctggt tcgcgaggcg cggccaccag gtgcacctcc tcaccgtgca
      661 gcccggcccg attccgggcg tgaccgtcca cgacatcacc acgcaccacg gccccaagcc
      721 gcttcgttac ctgaagagcc tgggacgcgt gaaggagatc ctcaaggtcc tcgaccccga
      781 cctcctgcac acccatttcc tgaccggata cggatactgg gggcattaca gcggccggaa
      841 gccccacatg ctgaccgtgt ggggggatga cgtctacgtc acgccgttcg agtcgccgct
      901 caagaactgg ctggcacggc aggtgcttcg gggcgccgat tacctgaccg gcgacagcgc
      961 cgacatcatc gaggcgtgca tcaagctcgg cgcccgccgc gagacgagcg aggtggtgca
     1021 gtggggcgtg gatttcaccc gcttccatcc tgacgccgcg gggccggtgc gggatgcgct
     1081 cggcattcca acggaggcgc cggtggtcct gtccacgcgc tcgttcacgc aggcctacta
     1141 caacatcgac gtcatcatcg attcgatccc gcttgtccag aagaccttcc cggaggtcat
     1201 ctacatcatc gccggcaacg agggttcgga cgaggcgatc cgggcccggg cccgtgaaag
     1261 gggcgtggaa gccgcctgcc ggttcgtcgg ccggattccc cacgccgacc tgccgagctt
     1321 cctcacctcc tcgcatgtct acctcaccgt gccgtcggtc gacgccaccg cggtcagcct
     1381 cctcgaggcg atggccaccg gaacggcgat catcgccagc gacctcccct cggccaccga
     1441 gtggatccgg gacggggaga cggggcgggt ggtgaaaccg cgcgaagtgg aggggctggc
     1501 ccgggtcacg gtcgagctcc tggccgatgg cgggctgcgg cggcggctgg gcgaacgggc
     1561 cgtctcgctc gtccgggacg gcgccgatca cgacaggaac atggcgcggg tcgaggcgat
     1621 ctaccgggaa ttggtggcga agaagta
//
LOCUS       DASVOD010000515         2167 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_503728, whole genome shotgun sequence.
ACCESSION   DASVOD010000515 DASVOD010000000
VERSION     DASVOD010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503728"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VF720_11505"
     CDS             complement(<1..744)
                     /locus_tag="VF720_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880030.1"
                     /translation="MTRFLPRGQVSLLAALLGLFLAGPSAAMTPDEIRAGIEQGRLLD
                     AFDAATLLVAAEPTNCQAWLLLGDANRKLARVGAAAQAYRTGLEACPEDKDLLKTYGL
                     MLDESEQYDEAARVLSRYWALDSSDAALGARIGAACYRAGRCPDGRKVYDAILAANPG
                     SSGDRLAYAQLLSRTCKDFEAAKVQFEKLVADSPNDPNVACAWVYALADAGQTDEAAK
                     VATSALAASGGTAGCLYAAWGRALEGAADS"
     gene            complement(791..1615)
                     /locus_tag="VF720_11510"
     CDS             complement(791..1615)
                     /locus_tag="VF720_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880031.1"
                     /translation="MTTTRTESLAGRFFWVPLLLIGGLLAVDQVKGPVFHPTPGKKPR
                     PPRSGDVAAAAAASGDRRDNTTSPAANDPAAGSTTGSTLTAQDERVGGLLSTTANPDE
                     ASKKVEGIRNQLKVNPKDYRLHYELGNALYDAGDQPAAISAYKKAIELNPKFVKAYVN
                     LGETLRERGDVKEAVSTLETALTLDARSDVAHAALGYAYYTDRKYPDAMNQFQEALSL
                     NPKSVHATYYMGMAFADAQIYREAITWWEKVCEIDPNSDACLEARENVNLLKRIVN"
     gene            complement(1834..>2167)
                     /locus_tag="VF720_11515"
     CDS             complement(1834..>2167)
                     /locus_tag="VF720_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7880032.1"
                     /translation="RMGEMELPLQERFLGWLISRRDVIVTGPKSAGPERVPTISFRSR
                     RKSPAAILPAVDRSGVAIRHGHMYSYRLCRALGIDTDEGVIRVSFVHYNTPAEVDRLI
                     GVLEEALA"
BASE COUNT          335 a          714 c          744 g          374 t
ORIGIN      
        1 gctgtcggcc gcgccttcaa gggcccgccc ccatgccgcg tacaggcagc cggccgtgcc
       61 gccgctggcc gcaagcgcgc tggtggccac cttcgcggcc tcgtcggtct ggcccgcgtc
      121 ggccagggcg taaacccagg cgcaggccac gttcgggtcg ttcggcgaat cggccacgag
      181 cttttcgaac tgcaccttgg ccgcttcgaa atccttgcag gtgcgcgaaa ggagctgcgc
      241 gtacgccagg cgatccccgc tcgagccggg gttcgccgcg aggatggcgt cgtacacctt
      301 ccgtccgtcc gggcagcgcc cggcccggta gcaggccgcg ccgatgcggg cgcccagggc
      361 cgcatccgac gaatcgaggg cccagtaccg ggagagcacc cgggccgctt cgtcatactg
      421 ctcggactcg tcgagcatga gtccatacgt cttcaggaga tccttgtcct ccgggcaggc
      481 ctcgaggccg gtccgatacg cctgggccgc cgcgccgacg cgggcgagtt tccgattggc
      541 gtcacccagg aggagccagg cctggcagtt ggtcggttcg gccgctacga ggagggtggc
      601 ggcgtcgaag gcatcgagca ggcgcccctg ctcgattccg gcccggatct cgtcgggcgt
      661 catggccgcc gatgggccgg cgaggaagag gccaaggagc gccgcaagca ggctcacctg
      721 gccgcggggc aggaaacggg tcatcaagaa agctcctttc gactacgcca agcgaggcgc
      781 cccggcgacc tcagttgacg attcgcttca acagattgac gttttcccgt gcctcgaggc
      841 aggcatcgga gttcgggtcg atctcgcaga ccttttccca ccaggtgatg gcttcacggt
      901 agatctgcgc gtcggcgaag gccatcccca tgtaataggt ggcgtgcacc gacttgggat
      961 tgagcgagag cgcctcctga aactgattca tggcgtccgg atacttgcgg tccgtgtaat
     1021 aggcgtatcc gagcgcggcg tgggccacgt cgctcctggc gtcgagcgtg agggcggttt
     1081 ccagcgtgga cacggcttcc ttcacgtcgc cccgctcacg gagcgtttcg ccgaggttca
     1141 cgtaggcctt cacgaacttg gggttcagct cgatggcctt cttgtacgcg gaaatcgcgg
     1201 cgggctggtc gcccgcgtcg tacagcgcat tacccagctc gtagtgcagg cgatagtcct
     1261 tgggattgac cttgagctgg ttgcggattc cctcgacctt cttcgaggct tcgtcggggt
     1321 tggccgtggt ggagaggagc ccacccaccc gctcgtcctg tgccgtgagg gtggacccgg
     1381 tcgtcgaacc ggcggcggga tcattggcgg ccggcgaggt cgtgttgtcg cgccgatcgc
     1441 cactggccgc cgcggcggcg gccacgtcac ccgaacgcgg tggacgcggc ttcttgccgg
     1501 gcgtcggatg aaacaccgga cctttcacct ggtccaccgc caggaggccg ccgatcagga
     1561 gcagcggcac ccagaagaac cgtcccgcca gggactccgt gcgcgtcgtc gtcatctgtc
     1621 gattacctgt cagtacccat gtgtgagaca cggaagcgct ccagccggcg cgcatggcgc
     1681 ccgatagcct aaaacgaagg gagaagtcta cttcctgtcg tggaagggtg tcaatttcca
     1741 tcggcagagg ggccgccgcg ggtggtcggg aggggtctac acgaggaggt cggagcggtt
     1801 ccggggcccg accgcgtggt cggagatctc ctgctatgcc agggcctcct cgaggacccc
     1861 gatcaaccgg tccacctcgg ccggggtgtt gtagtggacg aagctcaccc ggatgactcc
     1921 ctcgtccgtg tcgatcccga gagcccggca gagtcggtat gagtacatgt gaccatggcg
     1981 aatggccacg ccggagcgat ccaccgccgg caggatggcc gccgggctct tgcggcgcga
     2041 ccggaaactg atggtcggca cccgttccgg cccggcactc ttcggccccg tcacgatgac
     2101 gtcccggcgg ctgatgagcc acccgaggaa tcgctcctgg agcggcagct ccatctctcc
     2161 catccgg
//
LOCUS       DASVOD010000516         7775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_398241, whole genome shotgun sequence.
ACCESSION   DASVOD010000516 DASVOD010000000
VERSION     DASVOD010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398241"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3342)
                     /locus_tag="VF720_11520"
     CDS             complement(<1..3342)
                     /locus_tag="VF720_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880033.1"
                     /translation="MGPSILDAVYRATAAAGAGNDSLFIIFSEDLNPGDPPVAADFNF
                     NGGLASGDVGGLSGGSVARVGRNLVVMSNFTTPYANGDSVQVSVSGALTGADGSVQTD
                     VSLIAIRTGPAIYRAEIIPNAYDSRPNGDVLRLYFTHEIASGLSLTDPRQDFVMAEFG
                     TGAANLTAALVGTKAVDITFGNQEGASSASHGKMLAQISKIRLIAGAIAGDFNGSGDG
                     ADENSTTAPFHTVDSIVDAAGGTYRGPYLLAAGYNSKATGPFTDDVLTLVFSDDVDPS
                     SVTPADFVLGGATFTQVTSNDGDHIIELRDLSAAPASVSIVAGVITDYQGFVNPTIAA
                     SVVASPIIVHTRYQDGGDSDPNTDIVEIYFDTPVIGSFADTTDFDYYGFVSNGLIVTD
                     NTDNDSLIALTGFTVSNNWEPGDRIGLRPGTNMTAGGPPTLPTGNGAVATNVGADATR
                     PIRDFSAPRQLHFVLNQTYFRDAQSIGGADTAYYAFNEVDVDDSAYFLLFTRRNSPVD
                     EIYIQTYLNNARFIANPNPGYQAAGDLNRNLIGTYDISPGLGTYSDGQTIQLGDNVNL
                     GICAVDADGNIALNTTSCLFVGALVAGPVPPPRDHDCVCFAPAPGQLDAKGVAGQTGA
                     AFADGCNPVAGQTTDTDMIHIIGDRNPDEHFIFGDAGSAIGADSIRVYDDAALTVLLG
                     SGPANPVTGTFAVFSIGQPTDDFVWVVAVDVVAGNSTLSSPTPIRNDLPIYDAVYAST
                     FRDPLNSLRRYSPGDTVRVLGAVTDTAGTPDVAYFPPGIHKSDLLAISADFRGFSTRA
                     GSDSVVFISLGANQNDEDNDWIDNGSTRFDNTVIAFNNVKDYPEPYVDENGDGVYNCG
                     ETFVDYSAAGYTAKIYDIGDQNLDSSDPEEHGWYYVEYWIDPNDATLVADPDGGDNFA
                     TLNDVNIPVRILDNAITSQNALNQLSDDIDATLNDRTLMSFTTDLSLDHLTNTALDSA
                     FVATLDALAPTVSEISYLEKQTSLDEASSYPAGNIITPGDHAYELPSGMATPYINFVD
                     STLSDDDVIFVAVQIDDSPNSSSEPDAGWKYLSLDPSGDKADVGYPGIRNIDDDYDTT
                     GVFGVNVKTNGLDDDEDGVVDEDGEG"
     gene            complement(4291..5382)
                     /locus_tag="VF720_11525"
     CDS             complement(4291..5382)
                     /locus_tag="VF720_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX7880034.1"
                     /translation="MRIAIMGFPQSGKTTLFNVLARAHAPTGQFASSSGGLNIGTVSV
                     PDARLEKLRDLFSPKKYTPARVEYVDLAGPPPGKGEANAEALYPPQLRAAELIVCCLR
                     GFRDESVVHPNGSIDLERDRRNFDDEIIFRDMTVLDTRIQRLEKSGKLGAASKEDPKE
                     LPLLKRCREALEAETPLREIEFDADDEKRLKGFQLLTAKPILYVANIDESDLPDKSEA
                     FRRMSTGAQRPRSAMVAVAAKTEMDLAALEGNERTEFMELLGITESSLDRLIRISYDL
                     LGLCSFFTVGPDEVRAWTIPARSRAVDAAGEIHSDLARGFIRAEVVRWDELLAAGGTG
                     PLREAGKLRLEGKEYRVEDGDILNIRFSV"
     gene            complement(5445..5517)
                     /locus_tag="VF720_11530"
     tRNA            complement(5445..5517)
                     /locus_tag="VF720_11530"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5482..5484),aa:Lys,seq:ttt)
     gene            complement(5522..6802)
                     /locus_tag="VF720_11535"
     CDS             complement(5522..6802)
                     /locus_tag="VF720_11535"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEX7880035.1"
                     /translation="MPARIILGAQWGDEGKGKFVDLLTREADYVVRYQGGSNAGHTVV
                     VGEETFILHLIPSGILHPGKVGVLGNGVVIDPASLLKEIEGLTARGIDTEGRIRISDR
                     AHVILPYHKTIEAFEEDREGGAGAFGTTRRGIGPAYRDKASRNGIRMIDLFDEAVLRA
                     RIEANVDWVSHWLTRDGGRRPDLDPAALFDQYRAFGERLAPMLTDVTRLLNEALRKGT
                     PILLEGAQGTLLDVDHGTYPFVTSSSASAGGALIGCGIGPRWIEDVIGVAKAYTTRVG
                     LGPFPTEMPADLAATLRDQGAEFGATTGRPRRCGWLDGVGLRHAVDLNGLTRLAITKI
                     DVLSGMETLRIATGYETGSGWIADFPADTGLLGRCRPVYEDHEGWQESLSGCRRWDDL
                     PAAARRYLERISEITATPIGWVSVGSGRDQTIIL"
     gene            complement(6902..>7775)
                     /locus_tag="VF720_11540"
     CDS             complement(6902..>7775)
                     /locus_tag="VF720_11540"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880036.1"
                     /translation="RDALDRGHLAGAALDVFEQEPPPADHPLRTHPKVVAAPHLGAST
                     REAQENVSSELARQVLDYFGSGAIRNAVNMPGLSAEAYAKAKPYLDLAERLGLVAGSL
                     ADRGTWRRLAVEYRGDAADQPLQALTLAALKGVLSVRAGEAVNFVNARLKAQELDLAI
                     DESFDPVADGHSNRMSVRLEGDDARASVDGHVVRQLEPRLVAIDGFELEAVPAGPLVV
                     LRNDDVPGVVGAIGTALGAAGLNIARISWGRDAAGGMALTVIGLDQPASAEVLATLAA
                     DRRVRGVVGLVLPA"
BASE COUNT         1274 a         2431 c         2653 g         1417 t
ORIGIN      
        1 accttcgccg tcttcatcga ccacgccgtc ttcgtcgtcg tcgagaccgt tggtcttgac
       61 gttcacgccg aacacgccgg tcgtgtcgta gtcgtcgtcg atgttccgga tacccgggta
      121 accgacgtcg gccttgtcac cggacggatc gagcgagagg tacttccacc ccgcgtccgg
      181 ctccgagctc gagttcggcg agtcgtcgat ctggaccgcc acgaagatca cgtcgtcgtc
      241 cgacagggtc gagtcgacga agttgatgta cggggtggcc atgcccgacg gcagctcgta
      301 ggcgtggtcg cccggcgtga tgatgtttcc ggccgggtag gacgacgcct cgtcgaggct
      361 cgtctgcttc tcgaggtagc tgatctccga caccgtcggg gccagcgcgt cgagggtggc
      421 gacgaatgcc gagtcgagcg cggtattggt gaggtggtcg agcgagaggt ccgtcgtgaa
      481 cgacatcagg gtgcgatcgt tgagcgtcgc gtcgatgtcg tccgacagct ggttcagcgc
      541 gttctgcgaa gtgatcgcgt tgtccaggat gcggaccggg atgttcacgt cgttgagggt
      601 ggcgaagttg tcgccgccgt ccggatcggc caccagggtg gcgtcgttcg gatcgatcca
      661 gtattcgacg tagtaccagc cgtgttcttc cggatcgctc gagtcgaggt tctggtcgcc
      721 gatgtcgtag atcttggcgg tgtaaccggc cgccgagtaa tcgacgaagg tctcaccgca
      781 gttgtagaca ccgtcgccgt tctcgtcgac gtagggttcc ggatagtcct tcacgttgtt
      841 gaaggcgatg accgtgttgt cgaaacgggt ggacccgttg tcgatccagt cattgtcttc
      901 gtcgttctgg ttggcgccga ggctgatgaa gaccacgctg tccgagccgg cccgcgtgct
      961 gaacccgcgg aagtcggccg agatggccag caggtcgctc ttgtggatgc ccggcgggaa
     1021 ataggccacg tcgggcgttc cggcggtgtc ggtgacggca ccgaggaccc ggaccgtgtc
     1081 ccccggcgag taacgacgga ggctgttgag cgggtcgcgg aacgtcgagg cgtacaccgc
     1141 gtcatagatg ggaaggtcgt tgcggatcgg cgtcggggag gagagcgtgg agttgcctgc
     1201 cacgacatcg accgccacca cccaaacgaa gtcgtcggtg ggctggccga tcgaaaagac
     1261 ggcgaatgta cccgtgaccg ggttggccgg gccggagccc agcagcacgg tcagggcggc
     1321 gtcgtcatag acacggatcg agtccgcgcc gatggccgag cccgcatcac cgaagatgaa
     1381 gtgctcgtcg gggttgcggt cgccgatgat gtggatcatg tcggtgtcgg tcgtctggcc
     1441 ggccaccggg ttgcagccat ccgcgaacgc ggcgccggtc tggccggcaa cgcccttggc
     1501 gtcgagctgg ccgggggccg gtgcgaagca gacgcaatcg tgatcgcgcg gcggcgggac
     1561 cgggccggcg acaagggcac ccacgaacag acacgaggtc gtgttcaggg cgatgttgcc
     1621 gtccgcgtca accgcgcaga tgccgaggtt gacgttgtcg ccgagctgga tcgtctgtcc
     1681 gtcggagtag gtaccgaggc ccgggctgat gtcgtaggtg ccgatcaggt tccggttgag
     1741 atcgccggcg gcctggtagc ccggattggg gttcgcgatg aagcgcgcgt tgttcaggta
     1801 ggtctggatg tagatctcgt cgaccgggga gttccgccgc gtgaagagga ggaaataggc
     1861 cgagtcgtcg acgtcgactt cgttgaacgc gtaataggcg gtgtcagctc cgccgatcga
     1921 ctgcgcatcg cggaagtagg tctggttgag gacgaagtgg agctggcggg gggccgagaa
     1981 gtcgcggatg ggtcgggtgg catccgcgcc aacgttggtg gctaccgcgc cgttgccggt
     2041 cgggagggtg ggaggaccgc ccgccgtcat gttcgttccc ggacggagac cgatccggtc
     2101 acccggctcc cagttgttgg aaacggtgaa gccggtcagg gcgatgaggc tgtcgttgtc
     2161 cgtgttgtcc gtgacgatca ggccgttgga cacgaaaccg tagtagtcga aatcggtcgt
     2221 gtcggcaaac gatccgatca ccggggtgtc gaagtagatc tcgacgatgt cggtgttcgg
     2281 gtcggaatcg cccccgtcct ggtaccgggt gtggacgatg atcggcgagg ccacgaccga
     2341 cgcggcgatc gtcggattga cgaatccctg gtagtcggtg atgacgccgg cgacgatgga
     2401 gaccgaagcc ggggccgccg agaggtcgcg gagctcgatg atgtggtcac cgtcgttgct
     2461 ggtgacctgg gtgaaggtgg caccacccag gacgaagtcg gccggggtga cgctggacgg
     2521 atcgacgtcg tcgctgaaaa cgagcgtgag gacgtcgtcc gtgaaggggc cggtggcctt
     2581 gctgttgtaa ccggccgcca ggaggtacgg accgcggtag gtaccgccgg ccgcgtcgac
     2641 gatgctgtcg acggtgtgga acggcgcggt cgtggagttc tcgtccgctc cgtcgccgga
     2701 accgttgaag tcaccggcga tggccccggc gatgagccgg atcttggaga tctgggccag
     2761 catcttgccg tgggaggccg agctggcgcc ttcctggttg ccgaaggtga tgtcgaccgc
     2821 cttcgtaccg accagggccg cggtcagatt ggcggcgccg gttccgaact ccgccatcac
     2881 gaaatcctgc cgcgggtccg tgaggctcaa cccgctggcg atctcgtggg tgaagtagag
     2941 gcgcagcacg tcgccgttcg ggcggctgtc gtacgcgttc gggatgatct ccgcacggta
     3001 gatggccggg ccggtgcgga tcgcgatcag cgagacgtcg gtctggacgc ttccgtccgc
     3061 gcccgtgagg gctccggaga ccgacacctg caccgaatcc ccattggcgt acggggtggt
     3121 gaagttggac atgacgacca ggttgcggcc cacgcgggcc acggaaccgc cggagaggcc
     3181 gccaacatcg ccggaggcca ggccgccgtt gaagttgaag tcggccgcca ccgggggatc
     3241 acccgggttc aggtcttcgc tgaaaatgat gaacagtgag tcgttaccgg ctcccgccgc
     3301 agccgtcgca cgatagacgg cgtcgaggat cgacggcccc atgagcttcg gagcgccatc
     3361 cgcgagcttg tagggcgcca atggccccag ctgcgtcgac gaccgaggag gcgccgcgcc
     3421 cgcgaacgaa gccgccataa ggcaaacggc aacgctcacc aacgcgaacg tgaaagacaa
     3481 cctgcctctc caactcatgc gaagaccctc ctttgctgac acccgaccct cattcggact
     3541 cccatcatcg cgatcgttgt tgactggact gcgtgaactc gccggaagcg aagttgacac
     3601 ctttcgcaag ggcaacaccg tcatctcctt cctagaaggc gccggacggc gccgcctcca
     3661 cgtttcggac ttctcgttct ctctatgtgc cgttggtttc attcagccgt ctttccggac
     3721 aaaagaaaat ctgcagaact ctcgttctcc gcttccgccc tttttaagcc tcaaactact
     3781 cgtcttgcag atctctcttc gtactgcttg tgctgtctgg ccgtctggct gcttttcctg
     3841 gtgtgctcct cgatggccct gcgtcgttcg tgaaccgccg gttacgccgc gtgcatcacg
     3901 cgatggcgca actcattcgt tgcaggatgt acttcctccg gacccatgca ctgtttggtc
     3961 ttcagtgccg ggactattgc atacgtcgaa aaaactcgtc aactggcgaa tatccacccc
     4021 atcgtgagag gttacttcgc gtgcctgctt gacttagttg ccgcgattga aagtgtcggt
     4081 tgaggtgcgg cttccaagtc gctctttggc aaaatcgcgc gtaagctagc acgccccaaa
     4141 aaggggtgtc aatcgcgttt gaaactcggg ttgtaggcgt gcggaaatga ctcccccacc
     4201 gtcacttgcc agttttcgac aggtgccccg caaggcttga gaaaaaagat tttttcgtgg
     4261 atttcggctt aacccgttgt ccggcaagga ctaaacgctg aatcggatgt tcaggatgtc
     4321 cccatcctcg acccgatatt cctttccctc cagccggagc ttgccggctt cccggagggg
     4381 accggtcccg ccggctgcca ggagttcgtc ccagcggacg acttcggccc ggatgaaacc
     4441 gcgggccagg tcgctgtgga tttccccggc cgcgtcgacc gcccggctcc gggcgggaat
     4501 ggtccaggcc cggacctcgt cgggcccgac cgtgaagaac gagcagaggc cgagcaggtc
     4561 gtaggagatc cggatgaggc ggtcgaggct cgattcggtg atcccgagca gctccatgaa
     4621 ttcggtgcgc tcgtttcctt ccaatgccgc gagatccatt tcggtcttcg cggcgacagc
     4681 caccatggcc gagcgcgggc gctgcgcacc ggtcgacatg cggcgaaatg cctcggactt
     4741 gtcgggcagg tcggactcat cgatgttcgc cacgtacagg atcggcttcg cggtgagcaa
     4801 ttgaaaacct ttcaaccgct tctcgtcgtc ggcatcgaac tcgatctcgc gcagcggcgt
     4861 ttcggcctcc agggcctccc ggcagcgttt caggagggga agctccttcg ggtcctcctt
     4921 gctcgcggcg ccgagcttcc cgcttttctc gaggcgctgg atgcgcgtgt cgagaaccgt
     4981 catgtcgcgg aagatgattt cgtcgtcgaa attccggcga tcgcgctcga ggtcgatcga
     5041 tccgttcgga tgcaccaccg attcatcgcg aaatccccgc agacagcaga cgatgagctc
     5101 ggcggcccgc agctggggcg gatagagcgc ttcggcgttg gcttcccctt tgcccggagg
     5161 cggcccggcc aggtcgacgt actcgacgcg ggccggcgtg tatttcttcg gggagaagag
     5221 gtcccggagc ttttcgagac gcgcatcggg caccgaaacg gtgccgatgt tgaggccgcc
     5281 ggacgaactg gcgaattgcc cggttggcgc gtgtgcgcgg gcgagcacgt tgaagagcgt
     5341 tgtctttccg ctctgcggaa aacccatgat ggcgatgcgc atgaagctcg gaactcccgg
     5401 tgtcggagag atccggccgg aagcggagct tccgaccggt taattgaccc gtgctggaat
     5461 cgaaccagcg actccctgat taaaagtcag gtgctctacc atctgagcta acgggtctga
     5521 actacaagat tatggtctgg tcgcggcccg accccaccga cacccagccg atcggcgtgg
     5581 cggtgatttc ggaaatccgt tcaaggtagc ggcgggccgc ggcggggaga tcgtcccagc
     5641 ggcggcagcc ggagaggctt tcctgccacc cttcgtggtc ctcgtacacc ggccggcaac
     5701 ggccgaggag cccggtgtcg gccggaaaat cggcgatcca gcccgaaccg gtttcatagc
     5761 cggtggcgat ccgcagggtt tccatcccgg acaggacatc gattttcgtg atggcaagtc
     5821 gggtcaggcc gttcaggtcc accgcgtgcc ggaggcccac cccgtccagc cagccgcagc
     5881 ggcgggggcg gcccgtggtg gcgccgaact cggccccctg gtcgcggagg gtggccgcca
     5941 ggtcggccgg catttccgtg gggaacgggc cgaggcccac ccgcgtcgtg taggccttgg
     6001 ccacgccgat cacgtcctcg atccacctgg gcccgatgcc gcagccgatc agggctcctc
     6061 ccgccgacgc gctggaggag gtgacgaacg ggtaggtccc gtgatccacg tcgaggagtg
     6121 ttccctgggc cccctcgagg agaatcgggg tgcccttccg caacgcctcg ttgagcaggc
     6181 gcgtgacgtc ggtcagcatg ggcgcgaggc gttcgccgaa ggcccggtac tggtcgaaaa
     6241 gcgcggcggg atcgagatcc ggccgccgac cgccgtcccg agtcagccag tggctcaccc
     6301 agtcgacatt ggcctcgatg cgcgcgcgaa gcaccgcctc gtcgaagaga tcgatcatgc
     6361 ggattccgtt gcggctggcc ttgtcccggt aggcgggacc gatgccgcgc ctcgtcgtgc
     6421 cgaaggcgcc ggcacccccc tcacggtcct cctcgaaggc ttcgatcgtc ttgtgatagg
     6481 ggaggatcac gtgggcccga tcgctgatcc gaatgcgccc ctcggtgtcg atgccgcgag
     6541 cggtgagccc ctcgatctcc ttgagcaggg aggccggatc gatcacgacg ccgttaccga
     6601 ggacccccac cttgccggga tggaggattc cggatgggat caggtggagg atgaacgttt
     6661 cctcgccgac caccacggtg tggcccgcgt tcgacccccc ctggtagcgc acgacgtagt
     6721 cggcctccct tgtcagcagg tcgacgaact tccccttccc ctcgtctccc cactgcgccc
     6781 cgagtatgat ccgagccggc atctgtcgtt tcctcgtcct gccggagcgc gcgggcgcgg
     6841 tccggccatc cggtgcattg atccgatcgc gaaaagccgg aggaggctat caagtccgcc
     6901 gtcaggcggg caacaccagc ccgacgacac cccggacccg ccggtcggcg gccagggtgg
     6961 ccagcacctc cgcggaggcc ggctggtcga ggccgatcac ggtcagcgcc atgccgccgg
     7021 ctgcgtcacg cccccagctg atgcgggcga tgttgagccc ggcggccccg agcgcggtgc
     7081 cgatggcgcc caccacgccc ggtacgtcat cgttccggag caccaccagc ggcccggccg
     7141 gcaccgcctc gagctcgaac ccgtcgatgg ccacgagtcg cggctcgagc tggcggacga
     7201 cgtggccgtc caccgaggcg cgggcgtcat caccctcgag ccgcacgctc atccggttcg
     7261 agtggccatc cgccaccgga tcgaacgact cgtcgatcgc aaggtcgagc tcctgggcct
     7321 tgaggcgggc gttgacgaag ttcaccgctt cgccggcccg gaccgagaga acccccttga
     7381 gcgcggcgag cgtgagtgcc tggagcggct ggtccgcggc gtcgccacgg tactcgacag
     7441 ccaggcggcg ccacgttccc ctgtcggcca gggagccggc cacgagaccg agacgctcgg
     7501 ccaggtcgag ataaggtttt gccttcgcgt acgcctcggc ggaaagcccc ggcatgttga
     7561 cggcgttccg gatggcgccc gaaccgaagt agtcgagcac ctgccgggcg agctccgacg
     7621 acacgttctc ctgggcctcg cgggtggagg cacccaggtg gggcgctgcc accactttcg
     7681 gatgcgtacg caggggatga tccgccggcg gcggctcctg ctcgaagacg tcgagcgccg
     7741 cgcccgcgag atgcccgcga tcgagcgcat ccctg
//
LOCUS       DASVOD010000517         1815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_710835, whole genome shotgun sequence.
ACCESSION   DASVOD010000517 DASVOD010000000
VERSION     DASVOD010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710835"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /gene="dnaN"
                     /locus_tag="VF720_11545"
     CDS             <1..722
                     /gene="dnaN"
                     /locus_tag="VF720_11545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX7880037.1"
                     /translation="TESLEKGVRRTAYAVSSDEVRQVLTGVLMQLGGGKLTMVATDGH
                     RLARSTFPGDAGGAERDVIMPPKALNQVVRLAAGTVSVGITISKSFAVFDLGSTTVYS
                     RLIDGSFPNYEQVIPKTNPRQFSVGREELIAGLRRVAVLADSSTHQIKMSVRPERLEL
                     AVNTADIGEGLEQIPVTYTGDRLDIGYNSSYLLELLRSVDTPNVTVHLNNATSAGIFK
                     PEGLPEGEDLLCLVMPLRLPD"
     gene            722..1024
                     /locus_tag="VF720_11550"
     CDS             722..1024
                     /locus_tag="VF720_11550"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX7880038.1"
                     /translation="MSLERVGKSLERLLAEAGLDRPLRGWLAIDAWPEVVGQAVARRA
                     RATGFQDGRLIVEVDSPAWVSQLGFLKRDYIVKINQRLGEGVIRDIQFIAARGGRG"
     gene            1057..>1815
                     /locus_tag="VF720_11555"
     CDS             1057..>1815
                     /locus_tag="VF720_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013718882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880039.1"
                     /translation="MGREPGTGQEYDAAKIQVLKGLEGVRKRPAMYIGSTSARGLHHL
                     VYEVVDNSVDEVLAGVCTDIEVTIHADNSITVKDNGRGIPVDEHPVQKRPAVEVVMTV
                     LHAGGKFDDKSYKVSGGLHGVGVSVVNALSEWLEVEVRRDGKRFRQRYARGEVVHDLE
                     VVGKSEDTGTTVKFKPDATVFEETEFSYDTLSQRLRELAFLNAGLKILITDERSGKKH
                     EFKFDGGIVEFVKTLNHAKETLHPDAIYLHKTRDE"
BASE COUNT          340 a          582 c          603 g          290 t
ORIGIN      
        1 ccaccgagtc gctcgagaag ggcgttcgcc gcacggccta cgccgtttca tccgacgagg
       61 tacgccaggt gctcaccggc gtgctgatgc agcttggcgg cgggaaactc acgatggtgg
      121 ccaccgacgg ccatcggctg gcccgctcca cgtttcccgg ggatgccggc ggtgccgagc
      181 gcgatgtgat catgcctccc aaggccctga accaggtggt tcgactggcg gccggcacgg
      241 tcagcgtcgg catcacgatt tcgaagagct tcgcggtctt cgacctgggc tccaccacgg
      301 tctactcaag gctcatcgac gggtcgttcc cgaattacga acaggtcatc ccgaaaacca
      361 acccgcggca gttctcggtc ggccgcgagg aactgattgc cgggctccgc cgcgtggcgg
      421 tgctggcgga ttcctccacg caccagatca agatgtcggt gcgcccggag cgcctggagc
      481 tggcggtgaa caccgccgac atcggcgagg ggctcgagca gatcccggtc acctacacgg
      541 gtgacaggct ggacatcggc tacaactcca gctacctgct cgaactgctg cgctcggtgg
      601 acacgccgaa cgtcaccgtg cacctcaaca acgccacgtc cgccggaatc ttcaaacccg
      661 agggattgcc ggagggcgag gacctgctct gcctcgtcat gccgctccga ctgcccgact
      721 gatgtccctg gaacgcgttg gaaaatctct ggaacgcctc ctggccgagg cggggcttga
      781 tcgtccgctg cgcggatggc tggccatcga tgcctggccg gaggtagtgg gccaggccgt
      841 ggcgcgtcgc gcacgggcca ccggcttcca ggacggtcgc ctgatcgttg aagtcgattc
      901 accggcctgg gtcagccagc tcggatttct gaaacgggat tacatcgtga aaatcaatca
      961 gcgcctcggc gagggcgtca ttcgcgacat ccagttcatc gccgcgagag gaggacgagg
     1021 gtgaggggtg actccggaca ggatcccctg gtcgacctgg ggcgggagcc cgggaccggc
     1081 caggaatacg acgccgcgaa gatccaggtt ctcaagggcc tggaaggcgt ccgcaagcgc
     1141 ccggccatgt acatcggctc gacgagcgcg cgcggcctgc atcatctcgt ctacgaagtc
     1201 gtcgacaact cggtcgacga agtgctggcc ggggtctgca ccgacatcga ggtgacgatc
     1261 cacgccgaca actccatcac cgtgaaggac aacggtcgcg gcatcccggt cgatgagcac
     1321 ccggtgcaga agcggccggc cgtcgaagtg gtgatgaccg tgcttcacgc cggcggcaag
     1381 ttcgacgaca agtcgtacaa ggtgtcgggc gggcttcacg gcgtcggcgt atcggtcgtg
     1441 aacgccctgt cggagtggct cgaggtggag gtgcgccgcg acggcaagcg cttccgccag
     1501 cgctacgctc ggggcgaagt ggtgcacgat ctcgaggtgg tcggcaagtc cgaagacacc
     1561 gggacgaccg tcaagttcaa gcccgatgcc acggtcttcg aggagacgga gttctcctac
     1621 gacacgctga gccagcgcct gcgcgagctg gcgttcctca acgccgggct gaagatcctc
     1681 atcaccgacg agcggagcgg caagaagcac gagttcaagt tcgacggcgg catcgtcgag
     1741 ttcgtgaaga cgctgaacca cgccaaggaa acgctgcatc ccgacgccat ctacctgcac
     1801 aagacgcggg acgaa
//
LOCUS       DASVOD010000518         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_398343, whole genome shotgun sequence.
ACCESSION   DASVOD010000518 DASVOD010000000
VERSION     DASVOD010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398343"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="VF720_11560"
     CDS             complement(<1..946)
                     /locus_tag="VF720_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7880040.1"
                     /translation="MRLMSSLLRVAVVLFVVVTGCSGGGSKSPSGAVRAKNPMPEEPY
                     LSDAPPGQYGGRLVLSMLGSPKTFNAMITNDSPTNDVISGAVWEGLLDFDNVTQEVKP
                     GLLSSWEHSADGLVWTLKLRKGLKWSDGHPLTVDDVLFTTAVLQDTSLHINAASLLTR
                     SGSFPPFAKVDDTTFTVTLKKPFGPFLHAISGFRVIPKHKLEGAWKAGRFAQTWGLDT
                     PPDSLVGSGPFTIARYVPNESITLKPNPWFYRVDSKQQRLPYLDELIWVIVPDQNAEL
                     IKFKSGETDGLYARSEDVEGLKGEAEAGHFTVSDLGPEY"
     gene            complement(1145..1837)
                     /locus_tag="VF720_11565"
     CDS             complement(1145..1837)
                     /locus_tag="VF720_11565"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008608612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX7880041.1"
                     /translation="MRRKSIVILSGSGISAESGLPTYRDSNGLWRNHNWMELASPEGW
                     ARQPELVLEFYNERRAKAWEVEPNAAHRAIAELEKHHDVVVITQNVDALHERAGSSHV
                     VHLHGELAWVRGSSPRARRYRVDGAPIHIGDRCEDGTQLRPDIVWFGEAVPKMEEAIA
                     LVRKASHLLVVGTSLVVYPAASLVGYAPDDAVKVLVGLEPETVPPGYEFRQGKATERV
                     PEVVAEWLSAAG"
     gene            complement(1837..2304)
                     /locus_tag="VF720_11570"
     CDS             complement(1837..2304)
                     /locus_tag="VF720_11570"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX7880042.1"
                     /translation="MSARNDTAGVIAPPPLMYLGTLAIALFLNWARPLPITPDAITRW
                     PGGAIAVAGLVLAIRGRRTMTEAGTNVNPYKPTITIVDSGPFGFSRNPLYVAMTIILT
                     GLTLAANTWWGIILALPLLLVMHFGVIRREERYLEAKFGEPYLAYRRRVRRYL"
     gene            complement(2407..>2971)
                     /locus_tag="VF720_11575"
     CDS             complement(2407..>2971)
                     /locus_tag="VF720_11575"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880043.1"
                     /translation="FHQVPGDELDLEAVTRYGFDIVQRPTGGRAILHDEELTYSVVAA
                     ITDPRVGGTLTQSHASISEIFRHALTQLGLPATLAGTAATATADPTDRSRVSAPCFAS
                     ATRTELLVDGRKILGSAQRRGAHAFLQHGSLLLGDGHLRLADCLRLTEGERTRWRERL
                     AASTTTVPRTTPLEELIRAIRDAWEEG"
BASE COUNT          523 a         1011 c          925 g          512 t
ORIGIN      
        1 catactcagg gcccaggtca ctgaccgtga agtgccctgc ctccgcctcc cccttcaatc
       61 cttcaacgtc ttccgaccgc gcgtacaggc cgtccgtctc gcccgacttg aacttgatca
      121 actcggcgtt ctggtccggc acgatcaccc agatcaactc gtccaggtac ggcaaccgct
      181 gctgcttcga atccacccgg tagaaccacg ggttcggctt cagcgtgatc gactcgttcg
      241 gcacataccg ggcaatcgtg aacggcccgc tccccaccag gctgtccggc ggtgtgtcga
      301 gcccccaggt ctgcgcaaag cgcccggcct tccacgcccc ctccagcttg tgcttcggaa
      361 tgacgcggaa gcccgagatc gcatgcagga acggcccgaa tggcttttta agggtgaccg
      421 tgaacgtcgt gtcatccacc tttgcgaacg gcggaaacga ccccgatcgc gtcaacaggc
      481 tggccgcgtt gatgtgcagc gacgtatcct gaaggaccgc cgtcgtgaac agcacgtcat
      541 ccaccgtcag cggatgcccg tccgaccact tgagcccctt gcgcagcttg agcgtccaga
      601 caagcccgtc ggcgctgtgc tcccacgacg acaggaggcc cggcttcact tcctgcgtga
      661 cattgtcgaa gtcgagcaat ccctcccaca ccgccccgga gatcacgtcg ttcgtcggtg
      721 aatcattcgt gatcatggcg ttgaacgtct tcggcgaccc gagcatcgag agcaccaggc
      781 gcccgccgta ctgccccggc ggggcgtcgc tgaggtacgg ctcttccggc attggattct
      841 tcgcgcgcac cgcgcccgac ggggatttcg atccaccgcc cgaacacccg gtcaccacca
      901 cgaacaacac cactgcaacc cgcaacaaac tcgacatcag ccgcatgaaa gtctcccgaa
      961 cgggaaaagt gacgtgggga cggatcggtg gaatgtagcg gagggggtgc ccaggcgcca
     1021 cccggccggg ctttggtatc tatagtagaa gcacctggcg tgccgatggg gtgtaaaatc
     1081 ggaataagta tttatgtcat atggagttag aggttgcgcg aggcacaagg ggtcctctgg
     1141 cgcctcagcc ggctgccgac agccactccg ccacgacttc cgggacccgc tccgtggcct
     1201 tcccctgccg gaattcgtag cccggcggca ccgtctccgg ctccagccct accagcacct
     1261 tcaccgcgtc atcgggcgcg tagccgacca gtgacgccgc cggataaacc acgagcgacg
     1321 tcccgacgac caggagatgc gaggccttcc gcaccagcgc aatcgcctcc tccatcttcg
     1381 gcacggcctc gccgaaccag acgatgtccg gcctaagctg cgtgccatcc tcgcagcgat
     1441 cgccaatgtg gatcggcgcg ccatccacgc ggtatcgccg cgccctgggt gacgagcccc
     1501 gcacccaggc cagttcccca tgcagatgga cgacgtggct cgagcccgct cgttcgtgca
     1561 gggcatccac gttctgcgtg atcacgacga cgtcgtgatg cttctccaac tctgcaatgg
     1621 cccgatgcgc cgcattcggc tccacctccc acgccttcgc ccgccgctcg ttgtagaact
     1681 ccagcaccag ctccggctgc cgggcccacc cctcggggct ggcgagctcc atccagttat
     1741 ggttgcgcca gaggccgttc gaatcacggt aggtcgggag gccggactcg gcgctgatgc
     1801 cggagccgga gaggatgacg atggacttgc ggcgcatcac aggtatcgcc gcacccgtcg
     1861 gcggtacgcc aggtagggct cgccaaactt cgcttcgaga tagcgttcct cgcggcggat
     1921 gaccccgaag tgcatcacca gcagcagagg cagcgcgagg attatccccc accaggtgtt
     1981 ggcggccagc gtaagtcccg tgaggatgat cgtcatcgcg acgtacagcg gattcctgct
     2041 gaaaccgaac ggtcccgaat cgacgatggt gatcgtcggc ttgtaggggt tcacgttcgt
     2101 cccggcctcg gtcatcgtgc ggcggccgcg gatggccagg acgaggccgg ccaccgcgat
     2161 ggcaccaccg ggccagcggg tgatggcgtc gggcgtgatg gggagcggcc tcgcccaatt
     2221 gaggaacagg gcaattgcca gggtacccag gtacatcagg ggtggtgggg cgatgacgcc
     2281 ggccgtgtcg ttcctcgcgc tcatgcagac gtcctttcgg tgcgcgtcgg actctccgtg
     2341 aggagagtaa accgaacggc gacgtaattt tccgcttatg tcggaagagc ggaagttcgt
     2401 cccgcgtcag ccctcttccc atgcatcgcg tatcgcccgg atgagctcct ccagcggagt
     2461 ggtgcgcggt acggtcgttg tgctcgccgc cagccgttcc cgccatcgtg tccgctcccc
     2521 ttccgtgagc cggaggcaat cggcgagccg gaggtgccca tcccccagga gcagcgagcc
     2581 atgctggagg aaggcatgcg cgccgcgacg ctgggcgctg ccgaggatct ttcgaccgtc
     2641 cacgaggagt tccgtgcgcg tggctgaggc aaagcagggc gccgacacgc gtgaacgatc
     2701 ggtcggatcc gccgtggccg tggcggcggt accggccagc gtggccggga gacccagctg
     2761 cgtaagcgcg tgacggaaga tttcggagat gctggcgtgg gattgcgtga gcgtccctcc
     2821 tacccgcgga tcggtgattg ccgcaaccac cgagtaggtc agctcctcgt cgtggaggat
     2881 ggcccgcccc cccgtcggcc gctggacgat atcaaaccca tagcgggtca ccgcctcgag
     2941 atcgagctca tccccgggca cctggtgaaa c
//
LOCUS       DASVOD010000519         3781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_763813, whole genome shotgun sequence.
ACCESSION   DASVOD010000519 DASVOD010000000
VERSION     DASVOD010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763813"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VF720_11580"
     CDS             complement(<1..413)
                     /locus_tag="VF720_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880044.1"
                     /translation="MGARKPDELKKTLFDEPIRTDLPQQLAARFSDVGVHSALADPTA
                     QFLSDLYVEGLALVRLVGTAARNPDKAPADLDQQVRRLAAYLDQRVGEGIGFVERTLA
                     FLDKRVDEDGDDTEAFEKNVARLERAGRIENAHGLA"
     gene            complement(512..1198)
                     /locus_tag="VF720_11585"
     CDS             complement(512..1198)
                     /locus_tag="VF720_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880045.1"
                     /translation="MRIPSIEHLESSVFKKSVNPRAASIFFGAFLDVGVEPTLAGKAA
                     SSLGRLYGEGVDFVRTVVADRGANALLYDDDFREDLKNRLVTLEVLSSDIPSHAAQIM
                     DILERRSGEPEDRVRVRTWVDLLRRLSTQCHVEAILDVPGETGIEGSPFGAGAVGRVF
                     KSLVILAGLVEFLEDDELPAGMSAVALADIFRETANRMRPAIVGDGRSWQGLVGYLND
                     FTSGGSLPPQ"
     gene            1539..2570
                     /gene="tsaD"
                     /locus_tag="VF720_11590"
     CDS             1539..2570
                     /gene="tsaD"
                     /locus_tag="VF720_11590"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012403242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEX7880046.1"
                     /translation="MILLGIETSCDETSAAILDGPRNVRSSVVSSQVEHLEYGGVVPE
                     LAARAHLRLLRPIVRMALDQAGITLEDVEAVAATQGPGLMGALLVGFGFARGLAWARN
                     RPFAAVNHLEGHLLSPILTDPAFEPPFLALLVSGGHTELVRSTKWLEYDVLGSTIDDA
                     VGEAFDKVSILLGLGYPGGPAIDRAAANGRPDAWPLPRARLAPGSLDFSFSGLKTAVR
                     TVVERRRKELGLGPADRLPANEQGDLAASFQAAAVGALLARLDQALTAYPTPRVVVAG
                     GVAANTALREGARALCERRGVAFHVPPLRYCGDNAAMIAAAGILRLEAGHRWPLSATP
                     VADFGRLPL"
     gene            2698..3663
                     /locus_tag="VF720_11595"
     CDS             2698..3663
                     /locus_tag="VF720_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7880047.1"
                     /translation="MSDGRILIIEDDDAIRTLLKFQLEAAGYDVVAAEHGQAGLATLV
                     DRVPDLIMTDLMMPEMDGQEVCRRVKSHFRTAHVPIVMLTARADLPTRVESLDHGAND
                     YLTKPYDRAEMLLRVRNLINWSRSQREANPLSGLPGNVAIERETMRRMNSGAPFLFLY
                     LDIDNFKAFNDAYGYQHGDEAIKLVGTLLVDEVTRHGQPTDFVGHVGGDDFVVLTVPE
                     ALGPMTTAILERFDAEARLLYSEEHRATGCIEVPNRRGEMEKFPIMTLTIAAVPSQRY
                     QVNHSAQLNDLAVELKRYGKSQKGSVVVHERRGIGQTRERTGTDG"
BASE COUNT          611 a         1297 c         1245 g          628 t
ORIGIN      
        1 gccaggccgt gggcgttctc gatgcggccg gcgcgctcga gccgggccac gttcttctcg
       61 aaggcttcag tgtcgtcgcc gtcctcgtcg acccgcttgt cgaggaaggc caacgtgcgt
      121 tccacgaagc cgatcccctc accgacgcgc tggtcgaggt aggcggccag ccggcggacc
      181 tgctggtcca ggtcggcggg tgccttgtcg gggttccttg ccgccgttcc gaccaggcgg
      241 acgagggcga gcccttccac atacagatcg gagaggaact gggccgtggg atcggccagg
      301 gccgagtgga cgccgacgtc cgaaaagcgc gcggcgagct gctggggcag gtccgtgcgg
      361 atcggttcgt caaacagggt tttcttgagt tcgtccggtt tacgggcgcc cacgtggacc
      421 tcgatggttc gggtttgctt gttggatcga tcgggtcgtg tcgtgcgggc ggccttgttg
      481 cgccgccgtt tctccggcgg cgaaccgccc gttactgcgg cggcagtgaa ccgcccgacg
      541 tgaagtcgtt caggtagccg accagcccct gccagctgcg gccgtcgccg acgatggcgg
      601 ggcgcatgcg gttggcggtt tcgcggaaga tgtccgcgag ggccacggcc gacatgccgg
      661 ccgggagttc gtcgtcctcg aggaactcta ccagaccggc caggatgacg aggctcttga
      721 agacgcgccc cacggccccg gcgccgaacg gcgatccctc gatgccggtt tcgccgggta
      781 cgtcgaggat ggcctccacg tggcactggg tggagaggcg ccggaggagg tcgacccagg
      841 tgcgcacgcg cacgcggtcc tcgggctcgc cggaacggcg ctcgaggatg tccatgatct
      901 gcgcggcgtg cgacgggatg tccgacgaga gcacttccag cgtgacgagc cggtttttca
      961 ggtcttcgcg gaagtcatcg tcatagagaa gagcgttggc gccgcgatcg gccacgaccg
     1021 tccgcacgaa atcgacgccc tcgccgtaca gccgtcccag cgaagaggcg gccttgccgg
     1081 ccagtgtcgg ttccaccccc acatcgagga aggcgccgaa gaaaatcgac gccgcccgcg
     1141 ggttcacgga cttcttgaag acgctgctct cgagatgttc gatggacggg atccgcattc
     1201 gctctccgtg gccgcttgcg cggccgttgc cgtcagtcga tccgggctcc gggcaaaggg
     1261 cgcccgacgg atcggccgct ccccgggaaa ttcgccgacc cataccggtg atcggccgtt
     1321 caggtccgat cttttgcggt aagccgaagc cggctggaaa agccgaatcg ccggatttgc
     1381 cggggaggat cgaaaaggct aagggaattt gagggcccct tcaaccgcgc accccccgga
     1441 tgaaccacgt gtgcccccat ctgtcggcgc gcacccctga tgagcggcgc gcacccccgt
     1501 tgaccgagcc cgttcgtcac ctatactgct cggttcccat gatccttctc ggcattgaaa
     1561 cttcatgtga cgaaacctcc gccgccatcc tcgacggccc gcggaacgtc cgctcgagtg
     1621 tcgtttcctc ccaggtggag cacctcgagt acggcggcgt cgtgcccgaa ctggcggcgc
     1681 gtgcccacct gaggctcttg cggcccatcg ttcgcatggc gctcgaccag gccggcatca
     1741 cactcgaaga cgtggaagcc gtggccgcca cgcagggccc gggcctcatg ggagcgctgc
     1801 tcgtcggttt cggattcgcc cgtggcctcg cctgggcccg gaatcgccca ttcgccgccg
     1861 tgaatcacct agaggggcat ctcctctcac ccatcctgac cgatcccgcg ttcgagccac
     1921 catttctggc cctcctcgtg tccggaggac acactgaatt ggtccgctcg acgaaatggc
     1981 tcgagtacga cgtgctcggc tccaccatcg acgacgcggt gggggaggcc ttcgacaagg
     2041 tgtcgatcct cctcgggctg ggctaccccg gcggcccggc gatcgatcgt gcggcggcca
     2101 acggccggcc cgatgcctgg cccttaccgc gggcccggct ggcccccggg agcctcgatt
     2161 tctccttcag cggcctcaaa actgccgtga gaaccgtcgt ggagcggcgg cggaaagaac
     2221 tgggcctggg ccccgccgac cgcctgccgg ccaacgaaca gggcgacctc gctgcctcct
     2281 tccaggccgc cgcggtgggt gccctgctgg cgcgtcttga ccaggccctc acggcatacc
     2341 cgactccgcg ggtggtcgtc gccggtggcg tggcggccaa caccgccctc cgggagggtg
     2401 cccgggccct ctgcgagcgg cgtggcgtgg ccttccacgt gccgccgctc cgctactgcg
     2461 gcgacaacgc cgccatgatc gccgctgcgg gcatcctccg gctcgaggca gggcaccgct
     2521 ggcccctgtc ggccactccg gttgccgatt tcggccgcct gccgctctga agcttcctgc
     2581 cacgcccttg aatccgggtt tcgtcgaggt caaggaccgc ccgcctccgg ccgataacgg
     2641 gcataagcgc cgtccctcgg gacggcaccg tcacccttcc aggagtcaaa caggcgcatg
     2701 tccgacggtc gaatccttat catcgaagac gatgacgcca ttcgcaccct gctgaagttt
     2761 cagctcgagg cggccggcta cgacgtggtg gctgcggagc atggccaggc cggtcttgcc
     2821 acgcttgtcg accgcgttcc cgacctcatc atgaccgacc tgatgatgcc ggagatggat
     2881 ggccaggaag tctgtcggcg ggtaaagagc cactttcgaa cggcccacgt gccgatcgtg
     2941 atgctcaccg cccgggccga ccttccgacg cgcgtcgagt cgctcgacca tggcgcgaac
     3001 gactacctca ccaagcccta cgaccgcgcg gagatgctgc tgcgcgtgcg caacctcatc
     3061 aactggagcc gttcccagcg cgaggcgaac cccctctcgg gccttcctgg caacgtggcc
     3121 atcgagcgcg agacgatgcg gcgcatgaac tccggcgcgc cgttcctctt cctgtacctc
     3181 gacatcgaca acttcaaggc gttcaacgac gcctacggct accagcacgg cgacgaggcg
     3241 atcaagctcg tcggcacgct cctggtggac gaagtgaccc gccacggcca gcccaccgat
     3301 tttgtggggc acgtcggcgg cgacgacttc gtggtcctca ccgtgcccga ggcgctcggg
     3361 ccgatgacca cagccatcct cgagcgcttc gacgccgaag cgcggctcct ctattccgaa
     3421 gagcatcggg ccaccggctg catcgaagtg ccgaaccggc gcggggaaat ggagaagttc
     3481 cccatcatga cgctgaccat tgccgcggtg ccaagccagc gctaccaggt gaaccactcg
     3541 gcccagctca atgacctggc cgtcgagctc aagcgttacg gaaagagcca gaaggggagc
     3601 gtggtggtcc acgaacggcg cgggatcggg cagacccggg agcgaaccgg caccgacggg
     3661 tagggatggg gcgattcccc cggatatttc ctaaagagaa ctcaagtcag tcatttggca
     3721 cgccgataga taggttcgaa agcgcagccc ggacggggcg gcggacaaat tcagacggag
     3781 g
//
LOCUS       DASVOD010000520         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_607243, whole genome shotgun sequence.
ACCESSION   DASVOD010000520 DASVOD010000000
VERSION     DASVOD010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607243"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF720_11600"
     CDS             complement(<1..435)
                     /locus_tag="VF720_11600"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEX7880048.1"
                     /translation="MKVEIDHILDANPRNIIGLMSGTSADGIDAALVAVSGCGAETRW
                     ELKGFVTVPFDEALRTEIIGIQDPKADRVLPRLSALNYRLGDVYAEACRKLVDRVGAR
                     MDDIHLIGCHGQTVFHDTGQSDRPDFVRSTFQCGEPGALAERT"
     gene            complement(437..1276)
                     /locus_tag="VF720_11605"
     CDS             complement(437..1276)
                     /locus_tag="VF720_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7880049.1"
                     /translation="MSSRMVGARDRGHWLIVGLLSLGLVTMLLPLWWMIGTSLKAVGA
                     GLGGLQDLFPWPLSLKNYRDILASGPFGRYFLNSAIVAVVVVAGNVLFSAMVGYAFAR
                     RKFPCKGPLWLGLLATLMVPKQVTMIPVYVLAAKLRLVDTYVALIAPFLVDAFNVFLM
                     RSYIEQLPDDLEEAARVDGASEWRIFFRVVFPLTRPALAVVGINTFLVNWNNFLYPLI
                     LTSSDKMRTLPVGLALYAQGENSVSWGHLMAGSCLSTLPILAVFVIFQRHIIEGITAG
                     AGK"
     gene            complement(1273..2112)
                     /locus_tag="VF720_11610"
     CDS             complement(1273..2112)
                     /locus_tag="VF720_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7880050.1"
                     /translation="MVFLLPWLVTFGVFALWPLLDSLWVSFTRYNPIQGPPRFTGLEN
                     YARLLKDPVFWTSLKITLIFVVGTIPLTTVGALLVALGLNRHLPMKALFRAGWFMPTI
                     VSMVVVSLVFKNFYSPFGFLNAVLKLVGLPGHAWLQDPNTALGAVMVMDVWAALGYYA
                     VIFLAGLQAIPAELYEAAALDGGSGWRKHWHITLPLLKSTTLFILVINTIRSFQVFIE
                     IFVMTRGGPLNSTLTTVYYLYDRAFNSFELGYASALAWALFAVTLGASLLYVRVLRHR
                     EGA"
     gene            complement(2149..>2471)
                     /locus_tag="VF720_11615"
     CDS             complement(2149..>2471)
                     /locus_tag="VF720_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880051.1"
                     /translation="REAALRLAAYLARGDNALALCRVAKSVQPAAAGMEKDDYYQANP
                     GERVFVEQLTTAVFPPNNPSWQEMENAVESWVEKAMFHQVSAEKALEEADREITALLP
                     RRNP"
BASE COUNT          478 a          770 c          835 g          388 t
ORIGIN      
        1 cgtgcgttcg gcgagggcgc cgggttcgcc acactggaag gtcgaccgga cgaagtccgg
       61 tcgatccgat tgtcctgtgt catggaagac tgtttgtcca tggcagccga tcaagtgaat
      121 gtcgtccatc cgcgcaccga ccctatcgac gagcttccgg catgcctccg cgtacacatc
      181 gcccagtcgg tagttgagcg ccgacaaccg cggcaacacc cgatcggcct tcggatcctg
      241 gatgccgatg atttcggtgc gcagcgcctc gtcgaacggc accgtcacga accccttcag
      301 ctcccagcgc gtctccgccc cgcatccgct cacggcgaca agcgccgcat cgatgccatc
      361 cgcactcgtc ccggacatga gcccgatgat gttgcgcgga ttcgcatcca ggatgtgatc
      421 gatttcgact ttcattttac tttccggcgc cggccgtgat cccctcgatg atgtgtcgtt
      481 ggaagatgac aaagacggcc aggatcggca aggtggagag gcacgatccg gccatcaggt
      541 gtccccagct caccgagttt tcgccctggg cgtagagcgc gagccccacc gggagcgtgc
      601 gcatcttgtc cgagctggtc aggatgagcg ggtagagaaa gttgttccag ttgacgagaa
      661 acgtgttgat gccgacgacg gccagggccg ggcgagtgag gggaaatacc acccggaaga
      721 agatgcgcca ctcgctggcg ccgtcgacgc gggcggcctc ctcgaggtcg tcgggcaact
      781 gctcgatgta cgacctcatc agaaagacgt tgaaggcgtc aaccaggaaa ggtgcgatca
      841 gggccacgta cgtgtccacg agccggagtt tggccgcgag cacgtacacc gggatcatgg
      901 tgacctgttt cggcaccatg agcgtggcga ggaggccgag ccagaggggc cccttgcagg
      961 gaaatttccg ccgggcaaag gcataaccga ccatcgcact gaagaggacg ttgccggcca
     1021 ccaccaccac ggccacgatg gcgctattca ggaaatagcg tccgaacggg cccgaggcca
     1081 ggatgtcgcg gtagttcttg aggctcagcg gccacggaaa gaggtcctgg aggccgccga
     1141 ggcccgcccc caccgccttg agcgaggtgc cgatcatcca ccagagcggc agcagcatcg
     1201 tgacgaggcc gagcgacaac agtcccacga tcagccagtg accgcgatcc ctggctccca
     1261 ccatgcgcga gctcatgcgc cctcccggtg gcggagcacg cgcacgtaga gaagcgaggc
     1321 gcccagcgtc accgcgaaga gggcccaggc cagtgcgctg gcgtaaccga gctcgaagct
     1381 gttgaaggcc cggtcgtaca ggtaatagac cgtggtcagc gtgctgttga ggggcccgcc
     1441 gcgggtcatc acgaagatct cgatgaagac ctggaagctg cggatcgtgt tgatcaccag
     1501 gatgaagagc gtggtcgact tcaggagcgg cagcgtgatg tgccagtgct tacgccagcc
     1561 ggagccgccg tcgagggcgg ccgcctcata cagctccgcg gggatggcct gcaggcccgc
     1621 caggaagatg accgcgtagt aaccgagcgc ggcccacacg tccatcacca tgacggcgcc
     1681 gagcgcggtg ttcgggtcct gcagccaggc gtgccccgga agcccgacca gcttgaggac
     1741 ggcgttcagg aagccgaacg gcgaatagaa attcttgaag acgagcgaga cgaccaccat
     1801 cgacacgatc gtcggcatga accagccggc ccggaagagc gccttcatcg gcaggtggcg
     1861 gttgaggccg agcgccacga gcagcgcccc cactgtcgtg agcggaatgg tgccgacgac
     1921 gaagatcagc gtgatcttga gcgacgtcca gaagaccggg tctttgagca gccgcgcgta
     1981 gttctcgagg ccggtgaagc ggggcggccc ctggatcggg ttgtaacggg tgaacgacac
     2041 ccagagtgaa tcgaggagcg gccacagcgc gaagacgccg aaggtgacga gccaggggag
     2101 gaggaagacc aaggtggaag gtccgtgccg cccgggacct cgcatcgatc agggattccg
     2161 tcgcgggagg agggcggtga tctcccggtc ggcttcctcc agggccttct cggcggacac
     2221 ctgatggaac atggccttct ccacccacga ctcgacggcg ttttccatct cctgccagct
     2281 cgggttgttg ggcgggaaca cggcggtcgt cagctgctcg acgaagacgc gctcgcccgg
     2341 gttggcctgg tagtagtcgt ccttctccat gccggcggcg gcgggctgca cgctcttggc
     2401 cacgcggcag agggccaggg cgttgtcgcc gcgggcaaga taggcggcca gcctgagtgc
     2461 cgcctcgcgc t
//
LOCUS       DASVOD010000521        10210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_243066, whole genome shotgun sequence.
ACCESSION   DASVOD010000521 DASVOD010000000
VERSION     DASVOD010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10210
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243066"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1861
                     /locus_tag="VF720_11620"
     CDS             <1..1861
                     /locus_tag="VF720_11620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7880052.1"
                     /translation="EEARQFIGEAIDSLTRVGESFELARTFLMAGEVGDGGEAEPAEW
                     IKRAFGIFLSLESRGYAARAAWAAADWFVRRGRLDEASVYLDRAMSLTSEDEEPELWS
                     RLIRLRREVENEYADHWVRAGGGLESFQELTRLFRGSSDLETALGEVLRLGISRSQSD
                     RGFVALGDGGAAMRIVARQGLSPVEAADLLTRLARPIKDLSADPKPVIVSQLTGDPRF
                     SLEERSALPGIRSLALLPLALPSGTPGLFYADRLRENDIGAFNQGDLNLLTLLTNLAS
                     LSVLEHQRKELLQENQSLKNQLWHQPYTEIVTRNARMIEILRMAEKVGDSGASILIEG
                     ETGTGKGLIAQSIHDQSRRRDKPFIQINCAALPEPLLESELFGHTQGAFTGAVREKLG
                     LFKEAEGGTLFLDEVDKTTETLQAKLLHVLDKKEIRPVGSTRWTTVDTRVICATNVDL
                     KDRIRSGRFLEDLYYRMNDFIIRVPPLRERPDDIALLVEHFLARFATQYGKSELVLLP
                     EVRRALNEYEWRGNVRELEKTIRRMVVLADDNEPIGLAALPFDRPSAAVIASANEASS
                     LRDEVARTERRVINDALERSRWNKSQAARDLKISYPCLLKKIKEHGLERRASH"
     gene            2266..3711
                     /locus_tag="VF720_11625"
     CDS             2266..3711
                     /locus_tag="VF720_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7880053.1"
                     /translation="MRREAASTYSDQFEYLAERFELIRLQADAYALRSEAGDFERTSR
                     WGDRHGKNHLLGSIDDLDRTIQRREEAIRRRHRESESHGPRFPIDLFVETHQLTREEY
                     DILLVLLFNESAGRGQGRITSGNDILNLLFTSHVEALRASRYLSASSTLLNHGLVRAL
                     PEEEGCNFLRSSYEVTEKTLREVLGVRARPVSGESAQAMTEVLPDGPFRLLQPRFSLE
                     RVVLESNIRQQLEDVLWQVRDGHVLFEDWELGRLLEKGRGVVILLSGLPGTGKTMSAE
                     ALAGSLERQLLIADYSQLESKWIGETEKNIVTVFRAARDSGAVLLLDEADAILAGRLE
                     GGHYNDRAYNRQVSLLLQELEQFEGLCILTTNRETSLDDGLARRLGASISFGVPGASE
                     RERIWKSLVSARVPLAADVDFTRLAIDFALCGGNIKNAVVSALRSAARRGAGTEVGQS
                     DFIRAAESEKSVFLKATRPIGFKPRERYTYS"
     gene            4188..4982
                     /locus_tag="VF720_11630"
     CDS             4188..4982
                     /locus_tag="VF720_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880054.1"
                     /translation="MAGLAILALTGSGCEDECWDHDCDCPDQDGRPPYTPSGLYSVTG
                     DGRIDLYWNGSPEEDLDFYRVYVSPDRDGPFESIGETGDNHFTDFGAVNSRTEWYAVS
                     AVDLCGHESLITGDDVFDTPRPDGQDLELFSRFGGYPERSGWDLVNRERQPSGAPGTD
                     VWIEGAGSQLDMVAAPGADIQDGGFGAPESVNWAPLEGWSPSGRVELVAGHNVVVHTS
                     DGYYGRFHVIDVRNGRVTVDWAVQLDRGNRELSATPPTESVGPAGQ"
     gene            4995..5774
                     /locus_tag="VF720_11635"
     CDS             4995..5774
                     /locus_tag="VF720_11635"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX7880055.1"
                     /translation="MKSLSGVRAAAAIAAVLGVSVNLGPALAAPPPPPPANGGRTSPN
                     RVIEAPTGPSRVVEVPVPRPGRPLDVDVQLIGGDGRLVYPGQQVGFTFRVNRDAYVVV
                     YNIDTEGRTHLLYPRNRWDAEYVRGGTTHFLPGRGSDYRLVADGPAGEEFVVAVASDH
                     PIADNWVNCWDGLAGATEYDRLGSTSRRMGFVRGDRFAAMDRVATRLVEVPVEPVGYG
                     TARSYTSFFIGDPNCRQDYRGNGKGRGKGNGNGSWQSRRGR"
     gene            5958..9188
                     /locus_tag="VF720_11640"
     CDS             5958..9188
                     /locus_tag="VF720_11640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HEX7880056.1"
                     /translation="MIARACRSLFVAVFLAALTSVTSWAEPRPAVPRPCMVPPVPQKK
                     SLPPPLGGAADPSRIFKGYRQRSIGVRTTPLGEGDGAPITLGNLRILAIRVDFSDQEM
                     DSTTAYFERLLLFQREHYGTISDGQFTLQHTLAPSVYRMPQPMAWYGLDDSIGVRQAI
                     LCWDAIRAADPEVNFNDYDQVVLFHAGPGQESDVLNDSLEQIWSVFFRMEDLVYYLPY
                     SGVTGGIPTDDRSAEGDTVFVADMPMFPELETQDGFVFSPVGVVCHELGHALGLPDLY
                     DTVAPENQVFAESQGIGSWDLMAAGTWNANGFVPAEFSAWSKVFVGWIDPVVITADQA
                     VTLSAVEIDRRHGVVKVPLGGDEYLLIENRYQDSNGDGRFNFTENDSSKCRYEGAGPD
                     SMLVCDFDFYTDSYADAEWDWWLPGEGTGSGLLIWHIDDSVIEENLPYNTVNAVADHK
                     GIDLEEADGIQDMDSPGQDRDSFGSPYDSYRAGWVDRFAPDTTPDSDAYYGIESGVTV
                     DQVSAAGARMTFRVSFGAGTDHWPVDLLAPVGGNHVAVGQLDTDSAREVVVADRAGNL
                     YVFDPDGTGAWSAPDGEPFASVGVPLGAPLVADLDGDDVGDVVVPGEDGRIFGWGGPN
                     GRPLGPLHDGVLVTSSNPIPSLELFGADLNPSTPGVEFGFGGARMPGSDLSRFEIYGV
                     SLDGRPVRRGVGSLRGGNGQLPAVVVDIDGDGHQEIVASVRADSSDTTGTVQLHLFDP
                     LPGTSREFVIAPLPDTAYYSAPVVGDLDRNGKMDVVITSSNGNIYVLEMSVEEGLPVF
                     HPMEGWPRYIFASGRDQVSLADVDNNGYLEVLALETGGVFHVFNYHGTSLVSLPVTVP
                     SEQRYFIESQLAPLVTDLTADGDPEFILPLDDGQVIGVDASGRQFEEWAYFGGGQDGA
                     SPVVEDLDGDGHIELIAVSRYLDRARLDVHTIGRAAGPPMWSAHRGSSARPGVLLPEE
                     YLPVANGPVLDDTFAMPNPARESTRFHYRVGEGVESVEIRIVDMLGRPVRNLAGSNFT
                     GTDNTVEWDLKGADGRAVAPGVYLARLTVTGPAGAEESTMKLAVLR"
     gene            9256..>10210
                     /locus_tag="VF720_11645"
     CDS             9256..>10210
                     /locus_tag="VF720_11645"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEX7880057.1"
                     /translation="MTKSHPVSVASRLALAGVMLAALGVFGIDSARAQASNSALTFLG
                     LGAGARAAALSGAVSGWIGDATAAYWNPAGISRLEGWDISGTHTEWLSDIRYEQVSVA
                     RNHGANGFGLSFGTAYAGDFDARDEVGNQEVSFGFSDLSLGVSYARSITETFSLGVTG
                     KYWRESIDDVAADGFAGDLGLAWRTPVDGLSAGGSIRNLGGKVQFDLAGADEFDLPQT
                     VQLGLAFERPVSALAGDILLAGDFISEKNGDPSMRFGAQYRYREGLSLMAGYRADFLD
                     DTADAGDGTSLEQSQDFSLGASYDRNFRFEYAYVPFKSDLGT"
BASE COUNT         1706 a         3356 c         3370 g         1778 t
ORIGIN      
        1 ggaagaggct cgccaattca tcggtgaggc catcgattcg ctcacgcgcg tcggcgaatc
       61 gttcgagctg gcccgcacct tcctgatggc aggcgaagtg ggcgacggtg gagaagccga
      121 accggccgag tggatcaagc gcgccttcgg catcttcctc tcgctggaat cacggggata
      181 tgcggcgcgt gccgcctggg ccgccgccga ctggttcgtc cgccgcggtc gcctcgatga
      241 agcctcggtc tacctggatc gcgccatgag cctcacgtcg gaggacgagg agcccgaact
      301 ctggagtcgc ctcatccgcc tccgtcgcga ggtggaaaac gaatacgccg atcactgggt
      361 gcgcgccggc ggcgggctcg agtcgttcca ggaactcacg cgactcttcc ggggctcgtc
      421 cgacctggag acggccctcg gcgaggtgct tcgcctcggc atctcccgca gccagagtga
      481 tcgcggattc gtggcactcg gcgacggcgg cgcggccatg cgcatcgtgg cccgccaggg
      541 tctctcgccg gtcgaggccg ccgacctcct cactcgcctg gcccgcccga tcaaggacct
      601 gagcgccgac cccaagccgg tcatcgtgag ccaactcacc ggggacccgc gattctcgct
      661 cgaggagcgc tcagcgctgc ctggcatccg gagcctcgcg ctcctgccgc tggcgctgcc
      721 gagcggaacg cccggcctct tctatgccga ccggctccgt gaaaacgaca tcggtgcctt
      781 caaccagggc gacctgaacc tcctgacgct gctcacgaac ctggcctccc tctcggtgct
      841 cgagcaccaa cggaaagagc tgctccagga aaaccagagc ctcaagaacc agctctggca
      901 tcagccctac acggaaatcg tcacccgcaa tgcccggatg atcgagatcc tccgcatggc
      961 ggaaaaggtg ggcgattccg gggcgagcat cctgatcgag ggagaaaccg ggaccggaaa
     1021 ggggctcatc gcccagtcca tccacgacca gagccgccgt cgcgacaagc cgttcatcca
     1081 gatcaactgc gcggccctcc ccgagccgct cctcgagagt gagctgtttg gccataccca
     1141 gggtgccttc accggcgcgg tgcgggaaaa gctcggcctc ttcaaggaag ccgaaggggg
     1201 aacgctcttc ctggacgagg tcgacaagac cacggaaacg ctgcaggcca agctgctcca
     1261 cgtcctcgac aagaaggaga tccgtccggt cggttccacc cgctggacca cggtcgatac
     1321 gcgtgtcatc tgcgcgacga acgtcgatct caaggaccgg atccgcagcg ggcgcttcct
     1381 cgaagacctc tactaccgga tgaacgactt catcattcgt gttccgccgc tccgcgagcg
     1441 tccggatgac atcgccctcc tcgtcgagca cttcctggcc cgcttcgcca cccagtacgg
     1501 gaagagcgaa ctggtccttc ttcccgaggt gcggcgcgca ctcaacgagt acgagtggcg
     1561 cgggaacgtg cgcgaactcg agaagaccat tcgtcgcatg gtggtcctgg ctgatgacaa
     1621 cgaaccgatc gggctggccg cgctgccgtt cgatcgcccg tcggccgccg tcatcgcgtc
     1681 ggcgaacgaa gcctccagcc tccgcgacga agtggccagg acggagcgcc gtgtgatcaa
     1741 cgacgcgctc gagcgctcgc gttggaacaa gtcccaggcg gcccgcgacc tcaagatcag
     1801 ctacccctgc ctgctcaaga agatcaagga gcacgggctg gagcgtcgcg cctcccacta
     1861 gcaacccccg tattgtttcg ttatcaactt tttttacaat gttaagttgt tgtctgcgtg
     1921 tgccttatgt gtaacctccc gggcgactgg caaacttgtt gataacaacg ccgctcgcga
     1981 caggggaggt ttgcgccacc gcgccttcgg gcacacctgc taactccttg tctttgaaca
     2041 acatccagtg acataaattc tgcttcgcga actgatgttt gttggcacac ccgatgcttt
     2101 ggttcaggct tcggaggggg gacctccaaa acaccgtcgg ccgatggcct gaagaccaga
     2161 ggaatcgcct ctgaacgccc caagtacact ggtcttcagt gcccatcggc gggtgtttct
     2221 tcccgaaggc tcaacgagtc gcgggatcgc gcagggggtc gaatcatgcg gcgcgaagca
     2281 gcgtcaacgt acagcgacca gtttgagtac ctcgccgaac gtttcgaact gattcgcctc
     2341 caggccgatg cctacgccct tcggagcgag gccggcgact tcgagcggac ctcccgctgg
     2401 ggcgaccggc acggcaagaa ccacctcctg ggctcgatcg acgatctcga tcgcaccatc
     2461 cagcgccgcg aggaagccat ccggcgccgc catcgggaaa gtgagtcgca tggcccgcgt
     2521 ttccccatcg acctgttcgt cgaaacccac cagctcaccc gcgaggagta cgacatcctg
     2581 cttgtgctcc tcttcaacga gtcggccggg cgcggtcaag gtcgcatcac ctcggggaat
     2641 gacatcctca atctcctctt cacgagccat gtggaggcgc tgcgcgcgtc acgctacctc
     2701 agcgcctcgt ccacgttgct gaaccacggg cttgtgcgcg ccctgcccga ggaggaggga
     2761 tgcaattttc tccgctccag ctacgaggtg accgagaaga cgctccgcga agtcctgggc
     2821 gtgcgcgccc ggccggtgtc cggggaatcc gcccaggcga tgaccgaggt gcttccggac
     2881 ggaccgttcc gtctgctcca gccgcggttt tcgctggagc gtgttgttct tgaatcgaac
     2941 atccgccagc agctcgaaga tgtgctctgg caggtcaggg acggccatgt cctattcgaa
     3001 gactgggagc tcggccgcct gcttgagaag ggacgcggcg tcgtcatcct tctctccggc
     3061 ctgccgggca ccggcaaaac catgtccgcc gaagcgctgg cgggcagtct tgagcgccag
     3121 ctcctgatcg ccgattactc ccagctcgag agcaagtgga tcggcgaaac ggaaaagaac
     3181 atcgtgaccg tcttccgcgc tgcccgcgac tccggggccg tgctgctgct cgacgaagct
     3241 gatgccatcc tggccggacg cctggagggc ggtcactaca acgaccgggc ctacaaccgg
     3301 caggtgagcc tgctgctgca ggagctcgaa cagttcgagg gactctgcat cctcaccacc
     3361 aaccgcgaga cgtcgctcga tgatggcctc gctcgccggc tcggagcctc gatcagcttc
     3421 ggcgtccccg gcgccagcga gcgcgagcgg atctggaaga gcctcgtctc cgcccgggtg
     3481 ccgctggccg ccgacgtcga cttcacgcgg ctcgccatcg acttcgccct ctgcggcggg
     3541 aacatcaaga acgccgtcgt atcggccctg cggagcgcgg cacgtcgcgg cgccgggacg
     3601 gaggttggtc agtccgattt catccgcgcc gccgagtccg agaagagcgt attcctcaag
     3661 gccacgcggc cgatcggttt caagccccgg gagcgttaca cctactcgta acgcccggtt
     3721 gaacgagtga cacgcccgac ccgcaatcgg ggccggtgcc cataagacca aggatgccgg
     3781 ctcccggctc tcagaccatg agatggggga tgtccaggct ggtaccgaga gataaccggc
     3841 atccgcggat ccaggcccgg cgcttcgcaa cgaagcgccg ggcgttttct tggttctcgt
     3901 ccgttgcccg gcccccgccc gttgcccggc ccgccgccgt tgttgtcgcg gccgcgcgcc
     3961 accgggggcc cgcgtccatc acctgcaccg ccctcacccg tttcctccgc caatccttag
     4021 ttccttgtcg aaaagtggct tatggcattt cggcggcggc atctctcatt ggcatcccga
     4081 ttgcgatgtc cggaagcgga cctgcaatgg ggaggcagac gtcaatgaaa gcgatcgggt
     4141 atgaggcgaa ggggaacggg cggccgagtc gtttggccgg ggtgttggtg gcgggtttgg
     4201 cgatcctggc gctgaccgga tcgggttgcg aagacgagtg ctgggatcac gactgcgact
     4261 gtcccgacca ggatggtcgc ccgccgtaca cgccgagcgg gctctactcg gtgaccggcg
     4321 acggacgcat cgacctgtac tggaacggtt cgcccgagga agacctggat ttctaccgcg
     4381 tgtacgtgag cccggaccgc gacggcccgt tcgaatcgat cggcgagacg ggcgacaacc
     4441 acttcaccga cttcggcgcg gtgaacagcc ggaccgagtg gtacgcggtt tccgcggtcg
     4501 acctgtgcgg ccatgaaagc ttgatcacgg gtgacgacgt gttcgatacg ccacggccgg
     4561 atggccagga cctcgagctc ttcagccgat tcggcgggta ccccgaacgc tccggctggg
     4621 acctggtcaa ccgggagcgg cagccgtccg gtgccccggg aacggatgtc tggattgaag
     4681 gcgccgggag ccagctcgac atggtggccg ccccgggcgc cgacatccag gacgggggat
     4741 tcggggcgcc ggaatcggtg aactgggcgc cactggaagg ctggtcgcca agcgggcggg
     4801 tggagctggt ggcggggcac aacgtggtgg tgcacacgtc ggacggctac tacggccgct
     4861 tccacgtgat cgatgtgagg aacggacggg tgacggtcga ctgggccgtg caactcgacc
     4921 gaggcaatcg tgagctgtca gcgacgccgc cgaccgaatc ggtcggtccg gcgggtcaat
     4981 aaggaggagt ggtcatgaag tccctgagtg gagtgcgtgc ggcggccgcc atcgcggcgg
     5041 tgctgggcgt gagcgtcaac ctgggcccgg cgttggcggc gcctccgccg ccgcccccgg
     5101 cgaacggcgg caggacgagc ccaaaccggg tcatcgaggc gccgacgggc ccttcgcgag
     5161 ttgtcgaggt tccggtaccc cgccccggcc gacccctcga cgtggacgtg caattgatcg
     5221 gcggcgacgg ccgtctcgtc tatcccgggc agcaggtcgg tttcacgttc cgggtcaatc
     5281 gtgacgcgta cgtggtggtc tacaacatcg acaccgaggg acggactcat ctcctctacc
     5341 cccgcaaccg gtgggacgcc gagtacgtgc gcggcgggac aacgcacttc cttcccggtc
     5401 gcggatccga ctaccgcctg gtggcggatg ggccggccgg cgaagaattc gtggtggcgg
     5461 tggccagcga ccatccgatc gcggacaact gggtcaattg ctgggatgga ttggccggcg
     5521 ccaccgagta cgaccggctc ggcagcactt ccaggcggat gggattcgtc cggggcgatc
     5581 gcttcgccgc gatggaccgg gtggcaaccc gcctggtgga ggttccagtc gaaccggtcg
     5641 gctacgggac cgcgcggtcg tacacctcgt tcttcatcgg tgacccgaac tgccgccagg
     5701 attaccgggg aaacgggaag ggacgcggga aggggaatgg aaacggctcc tggcagagtc
     5761 ggcgtggtcg gtaagtccga tcacttcata tagatgacca tgccgggtat cggccggcac
     5821 gggagaaaag cggcgccgcc cgggttcgac ccgggcggcg ccattcattg actggggcgc
     5881 aagcactccg atagactctt gtccctgcga cagttgcgcc attccggtcc gcccgcgggc
     5941 caccctgagg agagttcttg atcgcccgcg cctgccggtc ccttttcgtc gccgttttcc
     6001 ttgccgcact cacgagcgtc acttcctggg ccgagccgcg tcccgcggtg cccaggccat
     6061 gcatggtgcc gcccgttccc cagaaaaaaa gcctgccgcc gcctctcggt ggcgccgccg
     6121 atccttcccg catcttcaag gggtaccggc agcgttcgat cggcgtgcgg accacgcctc
     6181 ttggcgaagg ggacggtgcg ccgatcacgt tgggcaacct tcgaatcctg gccattcgcg
     6241 tggatttcag cgaccaggag atggattcga cgacggccta tttcgagcgc ctcctcctct
     6301 tccagcgcga gcattacggc accatctccg atggccagtt cacgctgcag catacgctgg
     6361 ccccctcggt ctaccggatg ccgcagccga tggcatggta cggcctcgac gactcgatcg
     6421 gcgtgcgcca ggcgatcctc tgctgggatg ccatcagggc cgccgacccg gaagtaaatt
     6481 tcaatgacta cgaccaggtg gtcctgttcc atgccggccc cggccaggag tcggacgtcc
     6541 tgaacgacag cctggaacag atctggtccg tcttctttcg gatggaggac cttgtctact
     6601 acctgccgta ttccggcgtg acgggcggaa tccccacgga cgaccggagc gcggaagggg
     6661 acacggtctt cgtggccgac atgccgatgt ttccggagct cgagacccag gacggctttg
     6721 tcttctcccc ggtgggcgtg gtgtgccacg aactcggcca cgcgctgggc ctgcccgacc
     6781 tctacgacac cgtggccccc gagaaccagg tctttgccga gagtcaggga atcggatcgt
     6841 gggacctgat ggccgcgggc acctggaacg ccaacggttt cgtccctgcc gaattctccg
     6901 cctggtccaa ggtcttcgtc ggatggatcg accccgtggt gatcacggcc gaccaggccg
     6961 tcaccctctc ggcggtcgag atcgaccggc ggcacggtgt cgtgaaggtc ccgcttggcg
     7021 gtgacgagta tctcctgatc gagaaccggt accaggactc caatggcgat ggacgcttca
     7081 acttcaccga gaacgacagc tcgaagtgcc gctacgaagg cgctggcccc gattcgatgc
     7141 tcgtctgcga cttcgatttc tacaccgaca gctatgccga tgcggaatgg gactggtggc
     7201 tgccgggcga aggaacgggc tcggggctcc ttatctggca catcgacgac tcggtgatcg
     7261 aggaaaacct tccgtacaac acggtgaatg cggtggccga tcacaaggga atcgacctcg
     7321 aggaggccga tggcatccag gacatggaca gccctggcca ggaccgcgac tccttcggga
     7381 gcccgtacga cagttatcgc gccggttggg tggatcggtt cgcgccggac accacgcccg
     7441 actccgacgc ctactacggg atcgagagcg gcgtgacggt ggaccaggta tcggccgccg
     7501 gcgcccggat gaccttccgg gtgagtttcg gagcgggaac ggaccactgg ccggtcgatc
     7561 tcctggcccc cgtgggcgga aatcatgtgg ccgtcggcca gctcgacacc gattccgccc
     7621 gcgaggtggt cgtggccgat cgtgccggca acctgtacgt cttcgacccg gacggaacgg
     7681 gcgcctggag cgccccggac ggggagccgt tcgcgtcggt gggtgtaccc ctcggcgcgc
     7741 cgctggtggc cgatctcgat ggggacgacg tgggtgatgt ggtcgtgccc ggggaagacg
     7801 gacggatttt cgggtggggt ggccccaatg gccggccact cggcccgctg catgacggcg
     7861 tgctcgtcac gtcgagcaac ccgattccgt cgctcgagct cttcggcgcc gacctgaacc
     7921 cgtccacgcc gggcgtggaa ttcggtttcg ggggtgcgcg catgcctggc tcggatcttt
     7981 cgcggttcga gatttacggc gtgagcctcg atggccggcc ggtccgtcgc ggggtcggtt
     8041 cgctccgtgg cggcaacgga cagctcccgg cggtggtggt ggatatcgac ggcgacggtc
     8101 accaggaaat cgtggcctcg gtccgcgccg actcgtccga cacgaccggg acggtgcagc
     8161 tccacctgtt cgacccgctc cccggaacga gccgcgaatt cgtgattgcg cccctgccgg
     8221 acacggccta ctactcggcg ccggtggtcg gcgacctcga ccgcaacggg aagatggacg
     8281 tggtgatcac ctcgtccaat ggcaacatct acgtgctgga aatgagcgtc gaggaagggt
     8341 tgccggtctt tcatccaatg gaaggttggc cgcgctacat cttcgcctcc ggccgcgacc
     8401 aggtgagcct ggccgatgtc gacaacaacg gttacctcga ggtcctggct ctggaaaccg
     8461 gcggcgtctt ccatgtcttc aactatcacg gtacgtcgct ggtgagcctc cccgtcacgg
     8521 tgccgtccga acaacgttac ttcatcgaat cgcagctggc gcccctggta accgacctga
     8581 ccgccgacgg tgacccggag ttcatcctgc cgctcgacga tggccaggtg atcggcgtcg
     8641 atgcctcggg tcggcaattc gaggagtggg cctatttcgg cggtggacag gacggcgcct
     8701 cgcccgtcgt ggaagacctg gacggtgatg gacatatcga gctcatcgcc gtgtcgcgtt
     8761 acctcgatcg ggcccggctc gacgtccata ccatcggtcg ggcagccggg ccgcccatgt
     8821 ggagcgcgca ccgggggtcg agcgcccgtc cgggcgtcct gttgccggaa gagtatcttc
     8881 cggtggctaa tggccccgtg ctcgatgaca ccttcgccat gcccaatccc gcccgggagt
     8941 cgacgcggtt ccattaccgg gtcggcgagg gggtggagtc ggtcgaaatc cggatcgtcg
     9001 atatgctggg gcggccggtt cgcaacctgg ccggatcgaa tttcaccggg accgacaaca
     9061 cagtggagtg ggacctgaaa ggggcggatg ggcgggcggt ggcgcccggc gtctacctgg
     9121 cgcggctgac cgtgacgggg ccggccggcg ccgaagaatc gacgatgaaa ctggcggtgc
     9181 tccggtgatc ggggccgaga tcggaagcct ctccccgtcc tataatgtcg gggtcgcctt
     9241 caaaggagtc caatgatgac caagtcccat ccggtgtccg ttgccagccg cctggccctg
     9301 gcgggcgtca tgctcgctgc cctgggagtc ttcggaatcg attccgcccg tgcccaggcc
     9361 agcaacagcg ctctgacctt tctcggcctc ggcgccggcg cccgcgcggc ggccctctcc
     9421 ggggcggtga gcggctggat cggcgacgcc acggccgctt actggaatcc cgccggcatc
     9481 tcgcgcctcg agggatggga tatctccggc acgcacaccg agtggctgtc ggacatccgg
     9541 tacgaacagg tgagcgtggc gcgcaatcat ggcgcgaacg gattcggcct gtcgttcggc
     9601 accgcctacg ccggcgactt cgatgcccgg gacgaggtgg ggaaccagga ggtctcgttc
     9661 ggcttctcgg atctctcgct cggtgtctcg tatgcccggt ccatcacgga gacattctcg
     9721 ctgggtgtca ccggcaagta ctggcgggaa tcgatcgacg acgtggcggc cgacggtttc
     9781 gcgggcgatc tcggcctggc ctggcggacc ccggtggatg gtctctcggc gggcggatcg
     9841 atccgcaacc tgggcggcaa agtgcagttc gacctggcgg gcgccgacga attcgacctg
     9901 ccgcagacgg tgcagctggg cctggcgttc gagcgccccg tctcggcgct ggccggcgac
     9961 atcctcctgg cgggtgactt catctcggaa aagaacggcg acccctcgat gcgtttcggg
    10021 gcccagtacc gctaccgcga gggactctcg ctgatggccg gctaccgtgc cgatttcctc
    10081 gacgacacgg ccgatgcggg tgacgggacc tcgctcgagc agagccagga tttctcgctg
    10141 ggcgcctcct acgacaggaa cttccgcttc gagtacgcct acgtgccgtt caagtcggac
    10201 ctcggcacga
//
LOCUS       DASVOD010000522         4764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_191050, whole genome shotgun sequence.
ACCESSION   DASVOD010000522 DASVOD010000000
VERSION     DASVOD010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191050"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..179
                     /locus_tag="VF720_11650"
     CDS             18..179
                     /locus_tag="VF720_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880058.1"
                     /translation="MLLSAKGRNVDQQMGFEVGAEDYITKPFSPRKLVDRINAILSQK
                     DSREHKLAS"
     gene            331..1065
                     /locus_tag="VF720_11655"
     CDS             331..1065
                     /locus_tag="VF720_11655"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX7880059.1"
                     /translation="MCRMLAISSRRTPPAAIFERFKALAFEGQTPTLGPDARGHNDGW
                     GMAAFVGERLIVEKSHLSITDPKSGFEEAVKRLSVPALWPGHVMIHLRRASAGMDVRT
                     ANSHPFHRKHEGRDWFLMGNGTVEGFDPTAEHGKIDFEYFFERVLENLPEGGELKPAV
                     DAGKAALVSRFPTYNSLVSVFMHPRGLEGYYDVADKFDRYHTLFKAESEGSVMLCSEA
                     LEVEGVTWDFYRDMGGVVSLPSFRKV"
     gene            complement(1069..1452)
                     /locus_tag="VF720_11660"
     CDS             complement(1069..1452)
                     /locus_tag="VF720_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880060.1"
                     /translation="MDARGLVYFVESNDPVRLTHLATLVMSGVLLDRPVVVVWMGRAL
                     GHLMSGGLDAVPTGDAAVATDETSPGALLREARQLGSVTFLACSADARRLNLDRAAFL
                     ARVDDVLAMTTILHRIEGAGTVLYL"
     gene            1505..3319
                     /locus_tag="VF720_11665"
     CDS             1505..3319
                     /locus_tag="VF720_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX7880061.1"
                     /translation="MPIRTLNELFTEAVRRYDRPDAFRMKRAGAWIDMSHREAAEAVR
                     EVAMGLAGLGVTAGDRVAILSENRVEWALADYAILALGAVNVPIYSTLTPPQVEFLLA
                     DSGARVVFVSNEAHRATIEAIRPRLESDIDLIIFDEADGGAVRTFASLRELGRTRNAA
                     DPAEFDRRCVAVTADTIASVIYTSGTTGRPKGVVLTHGNITSNVVSVRSILPIADGDS
                     CLSFLPLCHIFERMAGHYTMMDAGATVAYAESVDAVPENLLEVKPTVLVSVPRLYEKM
                     HSRVLDTVRRSSPLRRRLFHWAVDIGRRRSDCVLKHRPVPAMLAFQFRIAQRLVFNKL
                     KERLGGRIRFMVSGGAPLSRDVALFFHAAGLVILEGYGLTETSPVICVNRLEDPRLGT
                     VGQPVPGMEVRIAPDGEILVRGPGVMQGYFNAPEATQEAMADGWFHTGDIGLLDPDGF
                     LTITDRKKDLIVTAGGKNVAPQPIENLLKTDPFIMEAIVVGDRRPYCVALLAPDWARV
                     AAWARAEGIAAASNNGQPDPTALSREPRIRQFLMDRVNRVNSGLAPFETIKAIALIER
                     ELTVADGDLTPTLKVKRRDLAARFSTLIESLYGSQAPK"
     gene            complement(3824..>4764)
                     /locus_tag="VF720_11670"
     CDS             complement(3824..>4764)
                     /locus_tag="VF720_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX7880062.1"
                     /translation="ALSSGMAAIATLVLTELSSGDHLVAAADIYGGTRNLLNGVVRRM
                     GIETSWAAVDDPAAFRAAIGPKTKAIFVETPTNPTLRVADLDAIAAVAKESNVPLWVD
                     NTFATAVLQRPLDFGAAAVTHSATKAMAGHDDVTAGFIAGSQALADRCRETMKWLGGC
                     LDPHAAWLLERGLKTLGLRIRRQSDNALAVARWLSAHPAVSSVNYPGLPSHPSHALAA
                     RQMSGFGGILAFDLAGGATAVNRWAERLSLIRLAPTLGGVETIALVPAVSSHIRLTPE
                     ERTAVGISDGTVRISCGIEDPADLIADLERALGRHD"
BASE COUNT          801 a         1625 c         1563 g          775 t
ORIGIN      
        1 cccgcgacat cccggtcatc ctcctctccg ccaaggggcg gaacgtcgat cagcagatgg
       61 gtttcgaagt gggcgccgag gactacatca ccaagccctt cagcccccgc aagctggtcg
      121 atcgcatcaa cgccatcctg agccagaaag acagccgcga gcacaagctc gcttcctagc
      181 agccggcgcg tcccgcgccg gccgacagcc tgtcccgagc ccgatccctc cccgcaatcc
      241 tccggtataa tcgtgcgtcc gtagccgatt cctgaccttt cgccgggccg ccccgtggca
      301 cccggcttcc cgaaccggag gcctcttttc atgtgccgga tgctggccat ctcgtcccga
      361 cgcaccccac ccgcggccat cttcgaacgc ttcaaggcgc tggccttcga gggacagacc
      421 cccacgctgg ggcccgatgc ccgcggccac aacgacggct ggggaatggc ggccttcgtg
      481 ggggagcggc tcatcgtcga gaaaagccac ctctcgatca ccgatcccaa gtcgggtttc
      541 gaagaagccg tgaagcgcct ctccgttccg gcgctctggc ccggccacgt gatgatccac
      601 ctccggcgcg cctcggcggg gatggacgtg cgcaccgcca acagccaccc gtttcaccgc
      661 aagcacgaag ggcgcgactg gtttctcatg gggaacggca cggtcgaagg attcgatccg
      721 acggccgagc acgggaagat cgactttgaa tacttcttcg agcgcgtcct cgaaaacctg
      781 ccggaggggg gcgagctgaa gcccgcggtt gatgccggca aggcggcgct tgtctcgcgc
      841 ttcccgacct acaactcgct cgtgtcggtg ttcatgcacc cgcgcgggct cgaggggtat
      901 tacgacgtgg ccgacaagtt cgaccgctac cacacgctct tcaaggccga aagcgaagga
      961 agcgtgatgc tctgttccga ggcgctcgaa gtggaaggcg tcacctggga tttttaccgc
     1021 gacatgggtg gcgtggtgag cctgccgagt ttccggaagg tatagcgctt acaggtacaa
     1081 gaccgtcccg gctccctcga tccggtgcag gatcgtggtc atggccagga cgtcgtccac
     1141 ccgggccagg aacgccgccc ggtccaggtt caaccgccgc gcgtcggccg agcaggccag
     1201 gaaggtcacc gatccgagct ggcgggcctc acgcaggagg gcaccggggg atgtctcgtc
     1261 ggtggcgacg gccgcgtccc cggtcggcac agcatccagc ccccccgaca tcaagtgccc
     1321 caacgcccgc cccatccaga cgaccaccac cggccggtcg agcagcacgc cgctcatgac
     1381 cagcgtggcc aggtgggtca gccgcaccgg gtcgttgctt tcgacgaaat agaccagccc
     1441 tcgcgcgtcc atgcgcctcc atgattggtt gacgggatca gggcgaggat cataatcccg
     1501 ggcgatgccg atccgaaccc tgaacgagct cttcaccgag gccgtccgcc gctacgaccg
     1561 gcccgacgcc ttccggatga aacgcgccgg cgcctggatc gacatgagcc accgcgaggc
     1621 ggccgaggcc gtccgcgagg tggccatggg cctggccggg ctcggcgtga ccgcgggcga
     1681 ccgggtcgcc atcctctcgg agaaccgtgt cgagtgggcc ctggccgact acgccatcct
     1741 ggccctcggc gccgtcaacg tcccgatcta cagcacgctc acgccgccgc aggtggagtt
     1801 tctcctggcc gattcgggag cgagggtcgt attcgtgtcg aatgaggctc accgagccac
     1861 gattgaagcg atccgtccac gactcgagtc tgacatcgat ttgatcatct ttgatgaagc
     1921 ggacggcggc gccgtccgca ccttcgcgtc actccgcgag cttggacgaa cccgaaacgc
     1981 cgccgacccg gccgagttcg accgccgttg cgtcgcggtc accgccgaca cgatcgccag
     2041 cgtcatctac acctccggca ccaccggacg accgaaaggc gtcgtgctca cgcacggaaa
     2101 catcacgtcc aatgtcgttt ccgtgcgttc gattctgccc atcgccgacg gtgattcgtg
     2161 cctgtcattc cttccgctct gccacatctt cgagcggatg gccggtcact acacgatgat
     2221 ggacgccggc gccacggtcg cctacgccga gtcggtcgac gccgtgccgg agaacctcct
     2281 cgaggtgaag ccgaccgtcc tggtcagcgt gccgcgcctc tacgaaaaaa tgcactcccg
     2341 cgtgctcgac accgtgcgtc ggtcgtcgcc gctccgccgg cgcctgttcc attgggccgt
     2401 cgacatcggg cgccgacgca gcgactgcgt gctgaaacat cgaccggtgc cggccatgct
     2461 cgcattccag ttccgcatcg cccagcgcct cgtcttcaac aagctgaagg agcgcctcgg
     2521 cgggcggatc cgcttcatgg tgtcgggcgg cgcgcctctt tcgcgtgatg tagcactttt
     2581 cttccacgcg gccggcctgg tgatcctcga ggggtacggt ctcaccgaga cgtcgcccgt
     2641 catctgcgtg aaccgactgg aggacccgcg cctcggaacg gtgggccagc cggtgccggg
     2701 catggaagtg cgcatcgcgc ccgacgggga aatcctggtg cgcggtcccg gcgtcatgca
     2761 gggctacttc aacgctccgg aggccacgca ggaggccatg gccgatggct ggttccacac
     2821 gggcgacatc ggcctcctcg atcccgatgg cttcctgacg atcacggatc ggaagaagga
     2881 tctcattgtt acggccggcg gcaagaatgt ggcgccacag cccatcgaaa atctgctgaa
     2941 gacggatccg ttcatcatgg aagccattgt cgtgggcgat cggcgtcctt actgcgtggc
     3001 gcttctcgcg ccggattggg cgcgcgttgc ggcctgggct cgtgccgagg gaatagccgc
     3061 cgcatcgaac aacggccaac ctgatccgac ggcgctctcc cgcgaacccc gcatccgaca
     3121 attcctgatg gaccgcgtga accgcgtcaa ctccgggctc gcgccattcg aaacaatcaa
     3181 ggccatcgct cttatcgagc gcgaactcac cgtggccgac ggcgacctga cgccgaccct
     3241 caaggtgaag cggcgtgacc tcgccgcgcg attcagcaca ctcatcgaat ctctctatgg
     3301 gagccaggcc ccaaaataag tggcgaaccg gccggggagc gcggggcccc tcgatgggag
     3361 ccgcggcagg ccgattcgcc gaaagaggat ccgattggcc gggcgtggtt cccatcggct
     3421 gacatccgcc cacgcgaaga gcaccccccg tccactaggt gctcttcgac caccccgacc
     3481 aatcggaaaa gctgttgtgt tgctctggaa attcgagtgt cgcgctgcgt gctgttgctg
     3541 taactgctgg tgatgtcgtc gcgacgtcga ggcaggctcg gcgtaaaacc tcggctcggc
     3601 ccgggattgc gcccgggccg agccggatcg gaagcggtcg cgtgcccact tccaggtgag
     3661 cccccattcc caccttcgcc cgcgaagcat cgcaaggcgc atgccacgca tggaatgtcg
     3721 gcaacgggcg gcgcggcgcc cgtaactcaa atgtttttcc agttggcggt tcggaagtcg
     3781 tcagcggggc gcgggagttg cgtcgtgagg tgacgacgcc tcgtcagtcg tgacgaccga
     3841 gcgcccgttc gaggtcggcg atgagatcgg cagggtcctc gatgccgcac gagatccgca
     3901 cggtgccgtc ggagatgccg acggcggtcc gttcctcggg cgtcagccgg atgtgcgacg
     3961 acacggcggg caccagggcg atcgtctcca cgccgccgag cgtgggcgcc aggcggatga
     4021 gggagaggcg ctcggcccag cggtttaccg ccgtggcccc gccggccagg tcgaaggcca
     4081 ggatgccgcc gaagccggac atctggcggg ccgcgagcgc atgcgacggg tgcgagggga
     4141 gtcccgggta gttcaccgag ctcacggccg gatgcgccga cagccagcgg gccacggcca
     4201 gggcgttgtc gctctggcgg cggatccgga gcccgagcgt cttgaggccc cgttcgagga
     4261 gccaggcggc atgcgggtcg aggcaccctc ccagccactt catcgtttca cggcagcggt
     4321 cggccagggc ctgtgagccg gcgatgaagc cggcggtcac gtcgtcgtgg ccggccatgg
     4381 ccttggtggc ggagtgggtc acggcggcgg caccgaagtc gagcggccgc tgaaggacgg
     4441 cggtggcgaa ggtgttgtcc acccacagag gaacgttcga ttctttggcc acggcggcga
     4501 tcgcgtcgag gtcggccaca cgcaacgtag gattggtcgg cgtctcgacg aagatggctt
     4561 tcgttttggg gccgatcgcg gcccgaaagg cggccgggtc atccaccgcg gcccagctcg
     4621 tctcgatccc catgcggcgg accacgccgt tcaggagatt gcgcgtgccg ccatagatgt
     4681 cggcggccgc caccaggtga tcgccgctcg aaagctcggt caggacgagc gtggcgatgg
     4741 cggccattcc cgaggaaagg gcca
//
LOCUS       DASVOD010000523         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_139873, whole genome shotgun sequence.
ACCESSION   DASVOD010000523 DASVOD010000000
VERSION     DASVOD010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139873"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2113)
                     /locus_tag="VF720_11675"
     CDS             complement(17..2113)
                     /locus_tag="VF720_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880063.1"
                     /translation="MSDRRRSWIVASLASLVAGSMVLVAMLLATGGRWSLPLDDAFIF
                     FQYARQFASGHFFQYNGGDAPSGGATSVATLLIDSVGWLIGFRGDGMVVFALLSGAVG
                     LTLSLVAAWRLGKALGMERPWWAAALVGLNGPLLWGLFAGMDLPLYVASILWTLASWA
                     EESNEGTGRRRTITWAALAAASRPEGLVFAVLVTALLLVEQATRRREGDAARGNPFLP
                     FLPIVAGLVPALILYLFTGRATPTSLAVKGVLGAPGMDMGTFLGGAIEYFASIVRDIF
                     LGFEGADPQRLQGNNASGVLLYLAPLTLVLWFVGCLPGVAREVSHRRPGPCLLGALAL
                     LSALGVASLVVPRAWHWHRYLIPSYALILPFAALGLERIGAGLGRLAPDARPGVVQHV
                     TGAAWLILTLPGTFYFLLAFAQNSADIEFQQRTLARWCRANLPKEARLGVNDAGALRY
                     DGIHPILDFEGLVSPSLTESRRQGVASLWEALERLPESGLPTHLVVYPNWYDPALLRP
                     HRLLRQQRILRQTIAGGNPMNVYEADWELLRSGDLPAESDITAPLAERRLVDRVDVAD
                     RVNEKEHRYVYRAAEGVYQEMLELQPGGPDGRNIIDGGRLISDGESFELSGLSPGREA
                     VLVARSRGGFRVTVTVDGEEAGEWLEPGRAGANWVESSFTVRGGLIRGARARFDVRIT
                     ESHTAPWSIFHYWLYQ"
     gene            2295..>3425
                     /locus_tag="VF720_11680"
     CDS             2295..>3425
                     /locus_tag="VF720_11680"
                     /inference="COORDINATES: protein motif:HMM:NF016345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HEX7880064.1"
                     /translation="MRHQAVAAGLALLAGLASGLLSPPKALAQYFGKNKVHYNNFDWK
                     VIETEHFEVYYYDEEAETAKDAARMAERSYTRLAHVLRHQFKKPIPLVLYATHGQFQQ
                     TNILQELIDEGTGGVTEFAKQRVFLPFTGSYSELEHVLSHELVHAFQIDILYGQQQSL
                     LTNPFTFQAPGWFMEGMAEYLSLGGVDPNTEMWLKDGALQGYLVPISTLDYVYDIRVY
                     RFGQSLWEYIARRYGEEKVGDILRRVGHTRSVGRSIEFVLGVTVEKLSDDWLESIRVT
                     YLPQVAQYVRASDFGKRLTRHEKNQSNYNVVPALSPNGDKLTYISDRGLSIGLYLASA
                     IDGKELGRLAEGERRAGFESFRFFTSSADWHPDGRRVILPAKS"
BASE COUNT          608 a         1162 c         1168 g          487 t
ORIGIN      
        1 cccgaacgct ccggcgctac tggtacagcc agtagtgaaa aattgaccag ggtgccgtgt
       61 gggattccgt gattcgtaca tcgaaacggg ccctggctcc ccggatcagg cccccccgaa
      121 ccgtgaagga cgattccacc cagttggcgc cggccctccc cggctccagc cactcgccgg
      181 cttcttcgcc atccaccgtc acggtcaccc ggaacccgcc acggctccgg gccaccagca
      241 cggcctcgcg gccgggtgac agcccggaaa gctcgaacga ctcgccgtcg gagatcaggc
      301 ggccgccatc gatgatgttg cgaccgtccg gaccgcccgg ctggagttcc agcatctcct
      361 ggtacacccc ttcggccgcg cgatagacgt aacggtgctc cttctcgttg acgcggtccg
      421 ccacatcgac ccggtcgacc aaccggcgtt cggccagggg ggcggtgatg tccgactcgg
      481 cgggaagatc cccgctccgc agaagctccc agtcggcctc gtaaacattc attggatttc
      541 cgccggcgat cgtctgccgg aggatccgct gctggcgcag cagccggtgc gggcgcagca
      601 gggccgggtc gtaccagttg ggatagacga ccaggtgggt cgggagcccc gactcgggca
      661 accgttcgag ggcctcccag agcgaggcca ccccctgccg gcgtgactcg gtaagcgagg
      721 gggaaacgag cccctcgaag tcgaggatcg ggtggatgcc gtcgtagcgg agggccccgg
      781 cgtcgttcac accgagccgc gcctccttcg gcaggttggc ccggcaccag cgcgccagcg
      841 tgcgctgctg gaattcgatg tcggcgctgt tctgggcgaa ggccaggagg aagtagaacg
      901 tgccggggag ggtcaggatg agccaggcgg cgccggtcac atgctgcacc acgcccggcc
      961 tcgcgtccgg ggcgaggcgt ccgagcccgg cgccgatccg ctccaggccg agtgccgcga
     1021 acgggaggat gagggcatag ctgggaatga ggtagcggtg ccagtgccag gcgcgcggca
     1081 ccaccagcga ggccacgccc agcgccgaga ggagcgcgag cgccccgagg aggcagggcc
     1141 ccggccgccg gtgcgacacc tcccgcgcca cgcccgggag gcagccgacg aaccagagga
     1201 cgagcgtgag cggtgccagg tagaggagca cgcccgaggc gttgttcccc tggaggcgct
     1261 gcggatcggc gccttcgaaa ccgaggaaga tgtcgcgcac gatcgacgcg aagtactcga
     1321 tcgccccccc caggaaggtg cccatgtcca tgccgggcgc cccgagcacc cccttgaccg
     1381 ccagcgaggt gggggtggcg cggcccgtga agaggtaaag aatgagggcg gggacgaggc
     1441 cggccacgat cgggaggaag gggaggaacg ggttgccgcg cgccgcgtct ccctcgcgcc
     1501 ggcgggtggc ctgctcgacc aggaggagcg ccgtcaccag aacggcgaag accaatcctt
     1561 cgggacgact ggcggcggcc agcgcggccc aggtgatcgt gcgccgtcga ccggttccct
     1621 cgttcgactc ctcggcccac gaggccagcg tccagaggat cgaggccacg tagaggggga
     1681 ggtccatgcc ggcaaagagg ccccacagga gcggcccgtt caggccgacc agggcggccg
     1741 cccaccaggg gcgttccatg ccgagcgcct tgccgagtcg ccaggccgcc accagggaga
     1801 gggtgaggcc gacagctccc gagaggagcg cgaagaccac catgccgtcg ccgcggaatc
     1861 cgatcagcca tccgaccgaa tcgatgagga gggtggccac actggtggcg cctcctgacg
     1921 gggcgtcgcc gccgttgtac tggaagaagt gcccggaggc gaactggcgg gcgtactgga
     1981 agaagatgaa ggcgtcatcc agcggcaggg accagcgccc gccggtggcg agcagcatgg
     2041 ccacgaggac catgcttccg gcgacgaggg aggcgaggga ggcgacgatc cacgaccggc
     2101 ggcggtccga catggcgcgc atcttaccct gccgcccggg agcgtcaacc tcgggcgggg
     2161 gacaaccctt gattgacagc gattttgcag gattcctata ctcgaacgtg tacctgacgc
     2221 tcggccgtgc ccattcggcc ggcactaccg tcccaccagc cagctgccgg ccccggggga
     2281 ttccgaggtt ctgcatgcgg catcaagcag tggctgcggg gctggcactc ctcgccggtc
     2341 ttgcttccgg gcttctcagt cccccgaaag ccctggccca gtatttcggc aagaacaagg
     2401 tccattacaa caacttcgac tggaaggtga tcgagaccga gcacttcgag gtgtattact
     2461 acgacgagga agccgagacg gccaaggatg ccgcccggat ggccgaacgc tcgtacaccc
     2521 gcctggctca cgtcctccgt caccagttca agaagccgat tccactggtc ctgtatgcca
     2581 cgcacgggca gttccagcag acgaacatcc tccaggaact gatcgacgag gggacgggcg
     2641 gcgtcaccga gttcgccaag cagcgcgtgt tcctgccgtt caccggctcc tactccgagc
     2701 tcgagcacgt cctctcgcac gagctggtgc acgccttcca gatcgacatc ctctacgggc
     2761 agcagcagag cctcctcacc aaccccttca ccttccaggc gcccggctgg ttcatggagg
     2821 ggatggccga atacctctcg cttggcgggg tcgatccgaa cacggagatg tggctcaagg
     2881 acggcgcgct gcagggttac ctggtgccga tctccacgct cgactacgtg tatgacatcc
     2941 gggtgtaccg cttcggacag tcgctctggg agtacatcgc ccggcgctac ggcgaggaga
     3001 aggtaggcga catcctccgg cgtgtcggcc acacccggag cgtgggccgg tcgatcgaat
     3061 tcgtcctggg cgtcaccgtg gaaaagctct cggacgactg gctggaaagc atccgcgtga
     3121 cctacctgcc gcaggtggcc cagtacgtgc gggccagcga cttcggcaag cgcctcacgc
     3181 ggcacgagaa gaaccagtcg aactacaacg tcgtgccggc cctctcgccc aacggtgaca
     3241 agctcaccta catctcggac cgcgggctct ccatcgggct ctacctggcc tcggccatcg
     3301 acgggaagga gctgggacgc ctcgccgaag gggagcgccg cgccggcttc gagagcttcc
     3361 gcttcttcac cagttcggcc gactggcatc ccgacggcag gcgcgtgatc ctgccggcca
     3421 agtcg
//
LOCUS       DASVOD010000524         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_920853, whole genome shotgun sequence.
ACCESSION   DASVOD010000524 DASVOD010000000
VERSION     DASVOD010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920853"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2184)
                     /locus_tag="VF720_11685"
     CDS             complement(<1..2184)
                     /locus_tag="VF720_11685"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7880065.1"
                     /translation="MSRRLLVLGTAAALVLAAAWYGSGFNRAAPGSLTLLARDGRVTE
                     VSPQTFFRPAGGTVLSIPRTGTLIFPGGDDYRLFSREGEPVSVVFDVNWRLVGTGAAA
                     AWLLRGSDSTFVSAVVEAPLRRHARAIAAGLPMERIAATGGNALREAMDTALRSEADI
                     EFHVSIRSFALTPGATTVPVQPPVLVIGIDALDGRLLDDAIAAGHAPNLAGLIRRGVR
                     GDLATLTPMLSPLIWTSMATGVGPDRHGILDFLTPDPATGGLIPVTSAQRRAPAFWNV
                     ATAHGRPVDVVAWLATWPAESITGRLVSDRFGFLAYAAGASGSMPAPDMVFPPSLLEE
                     LKGISLAPTDVTFEATRRVLNVDRAAFEHARGHGFEKGELVNNYILTLATAESSTRIG
                     ERFLRASGPAGRAAITAVYYEYLDAVGHLFMRFASPRRGDVSADDWKRYGNAVTASYA
                     VMDEYVGRLLAAAGDSTTVLVVSDHGFHSGDGRPTGPSAIEGGQAARWHRNPGVIILA
                     GPGIRRGDRLQKAHVLDVTPTLLYLAGLPLADDLEGNLLAGAIDPGRLASSPATRVPH
                     WSLPPDVWAPPDLAMTPGGPANTTGTGRAATSLDSVKSWVNLGLVLEQRGDLAEAERF
                     YLRALDAAPGDPNATNNLANVWRRSGRVREAITFFERLTAEHPEFRPALQNLGACYLE
                     ADQPGKAIEWFNRALAAEPGNILARVNRGHAHLRIGQSDQAEADFR"
     gene            complement(2193..>2391)
                     /locus_tag="VF720_11690"
     CDS             complement(2193..>2391)
                     /locus_tag="VF720_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX7880066.1"
                     /translation="FRVEERTVEPDELLEAEEVFATSTSWEVLAVRSIDGRMVGDGRQ
                     GRITAAIHQSFRRLVAAETGR"
BASE COUNT          381 a          819 c          842 g          349 t
ORIGIN      
        1 ccggaaatcg gcctcggcct ggtccgattg tccgatgcgc aggtgcgcgt ggccgcggtt
       61 cacccgcgcg aggatgttgc ccggctccgc agccagcgcc cggttgaacc actcgatggc
      121 cttgcccggt tgatcggctt ccaggtagca ggctcccagg ttctgcagcg ccgggcgaaa
      181 ctccggatgt tcggccgtca gccgctcaaa aaaggtgatg gcttccctca cgcgcccgga
      241 ccgccgccac acgttggcca ggttgttcgt ggcgttggga tcacccgggg ccgcatcgag
      301 cgcccggagg tagaagcgct ccgcctcggc caggtcgcct cgttgttcga gcacgagtcc
      361 caggttcacc cagctcttca cggaatcgag cgaggtcgcg gcgcggccgg ttccggttgt
      421 gttggccggg ccgccggggg tcatcgccag gtcgggaggc gcccatacat caggcgggag
      481 gctccagtga ggcacccgcg tcgccgggct cgatgccagt cggcccgggt cgatcgcgcc
      541 cgccaggagg tttccctcga gatcgtcagc gaggggaagt cccgccaggt aaagcagtgt
      601 gggggtcacg tccaggacat gcgctttctg caggcgatcc ccacggcgga tcccgggtcc
      661 ggccaggatg atgactccgg gattccggtg ccagcgtgcc gcctggcccc cttcgatcgc
      721 cgacgggccg gtaggccgtc catcgcccga atgaaaaccg tgatcggaga cgacaagcac
      781 cgtcgtcgag tcaccggcag cggccaggag acgcccgaca tattcgtcca tcacggcgta
      841 ggaagccgtg accgcattgc cataccgctt ccaatcgtcg gccgacacgt cccccctccg
      901 cggcgacgca aagcgcatga aaagatgacc caccgcgtcc aggtattcgt agtacaccgc
      961 ggtgatcgcc gcgcgaccgg ccggcccgga ggcccggagg aagcgctcgc cgatccgcgt
     1021 ggacgactcc gcggtggcca gcgtcaggat gtagttgttg acgagttcac ctttctcgaa
     1081 tccatggccg cgcgcgtgct cgaaggcggc acggtccacg ttgagaacgc ggcgagtggc
     1141 ctcgaacgtc acgtccgtcg gcgcgagcga gatccctttc agttcctcga gcagcgaggg
     1201 ggggaagacc atgtcgggcg ccggcatcga gcccgaggcg ccggccgcgt aggccaggaa
     1261 gccgaagcgg tcggagacca gccgtccggt gatcgactcg gccggccagg tggccagcca
     1321 ggccaccacg tccaccgggc ggccatgggc cgtggccacg ttccagaaag cgggcgcgcg
     1381 tcgctgggcg ctcgtcaccg ggatcaagcc gccggtggcc ggatcgggcg tgaggaagtc
     1441 gaggatgccg tggcggtcgg gtccgacacc ggtggccatc gatgtccaga tgagcggtga
     1501 gagcattggc gtcagcgtgg ccaggtcccc gcgcaccccg cgccggatga gcccggccag
     1561 attcggggcg tgaccggcgg cgatggcgtc gtcgagcaac cggccatcga gcgcgtcgat
     1621 gccgatcacc agcacggggg gttgcaccgg caccgtggtt gcacccggcg tgagcgcgaa
     1681 cgaccggatc gatacgtgga attcgatgtc cgcttcggag cgcagggcgg tgtccatggc
     1741 ctcccgaagt gcgttgcccc cggtcgcggc gatcctctcc atcgggaggc ccgccgcgat
     1801 ggcccgggcg tggcgacgga gcggcgcctc cacgacggcg gagacgaagg tcgaatcgct
     1861 cccccgcagg agccaggcgg ccgccgcgcc ggtgccgaca aggcgccagt tcacatcgaa
     1921 caccacggaa accggctcac cctccctgct gaagaggcgg tagtcgtcgc cccccggaaa
     1981 gatcagcgtg ccggttcgcg gaatcgaaag gaccgtgccc cccgccggcc ggaagaaggt
     2041 ttgcggtgaa acttccgtga cgcgcccatc gcgcgccagc agcgtcagcg atccgggcgc
     2101 ggcgcggttg aatccgctgc cgtaccacgc ggccgccagg accagggccg cggccgttcc
     2161 gaggacgagc aggcgccgcg acatcccgcc gctcagcgcc cggtttcggc ggccaccagt
     2221 cgccggaacg actggtggat ggcggcggta atccttcctt gccgcccgtc gcccaccatc
     2281 cggccatcga tggagcggac ggccagcact tcccaggagg tggaggtggc gaagacttcc
     2341 tcggcctcga ggagttcgtc gggttcgact gttctctcct cgacacggaa c
//
LOCUS       DASVOD010000525         3798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_659626, whole genome shotgun sequence.
ACCESSION   DASVOD010000525 DASVOD010000000
VERSION     DASVOD010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659626"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF720_11695"
     CDS             complement(<1..792)
                     /locus_tag="VF720_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880067.1"
                     /translation="MSSPLLEIRDLATTFRTDGGTVRAVDGLDLALAAGETLGLVGES
                     GCGKSVTNLSILRLIPDPPGRIERGQVLLSGVNLLDLSEPEMRAVRGRRVAMVFQDPM
                     SSLNPFLTVARQLTEVLEIHERVSGREARRRAIEMLQRVGIADAGRRIDDHPHQFSGG
                     MRQRVMIAMALLCRPELLLADEPTTALDVTVQAQILELVKGLTRDFGTSVILVTHDLA
                     VVAGMAQRVAVMYATRHWHVNTVIYPTQDDALAWLARYQMTPALWL"
     gene            complement(789..1664)
                     /locus_tag="VF720_11700"
     CDS             complement(789..1664)
                     /locus_tag="VF720_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880068.1"
                     /translation="MPVPIALEPGASLWSDAWKRLRKNRMAVISGLVLLVVALLVVSA
                     PILPGVPDPNRQDLELGAVPPSPGHPMGTDILGRDLLARVLYGGQISLLVGLVATLVS
                     FLIGVTWGAVAGYFGGRLDNLMMRFVDVLYSLPYMFLVILLLVFFSRSIVMLFVALGA
                     VQWLTMARIVRGQVLSLKHREFVEAARAIGTSHAGIIFRHLIPNALGPIIVYATLTVP
                     AVMLQEAFLSFLGLGVQPPDASWGVLVNEGASVMGLFPWLVIFPGFALALTLFCLNFL
                     GDGLRDALDPHLRKE"
     gene            complement(1685..2602)
                     /locus_tag="VF720_11705"
     CDS             complement(1685..2602)
                     /locus_tag="VF720_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020149776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880069.1"
                     /translation="MIRFILRRLLWMIPTLWVVATITFFLMRAAPGGPFQAEKEIPED
                     VKEALMARYGLDKPLGEQYLTFLGNVVRLEFGPSYKTPGRTVREIIASGFPVSLELGS
                     WALLFALAVGIPIGVIAALNQNKPVDHASMAAALIGVSIPDFVLGPLLVFIFSLSLYW
                     LPPALWEGPSTKILPVITLGSVYVAYIARLTRSGMLEVIRQDWIRTARAKGLSESLIV
                     WRHALRGGLLPVVSFLGPAAARIVTGAVVVEKIFAIPGLGRYFVDGAFNRDYTLVMGV
                     VLFYATFLVLFNLIVDVAYGLLDPRVRLS"
     gene            complement(2599..>3798)
                     /locus_tag="VF720_11710"
     CDS             complement(2599..>3798)
                     /locus_tag="VF720_11710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7880070.1"
                     /translation="NAEAYQKGTIADVDSVGIAAPDDSTLIVTLENPTPYFLSLTCFY
                     TYMPVPRWCVEKFGERWSRPENVVVNGPFRCTFWRPANRFEFVRNERYWDKANVKLDG
                     IRAYAIDDLNTSLNLYKAGTTDWNPSGYLPAQYLPYVKDSADFHYAPYLAVYFYSFNT
                     TDGRMRNVWLRRALSWSVDREAICSQLFKGMKIPHGNFVAQGFAGYPYPAPQKFDPAF
                     ARECLAKAGYPGGKGLPKIEIMFNTSEDHRKIAEIIQAMWKDHLGIDVGLSNQEWGSY
                     LRNTTGLNYQVARRSWIGDYADPATFLDCYKTGDGNNRTGWSSAAYDALLLEASRTFD
                     PAARYAVLAKAEAILLDECPVMPIYNYTLTELKKPYVKGVEPNLTDTHNLKFAWIDRN
                     WEKTPTP"
BASE COUNT          692 a         1232 c         1281 g          593 t
ORIGIN      
        1 gagccatagc gccggcgtca tctggtaacg tgcgagccat gccaaggcat cgtcttgggt
       61 cggatagata acggtgttga cgtgccaatg gcgggtggcg tacatcaccg ccacccgttg
      121 cgccatgccg gccaccaccg ccaggtcgtg cgtcaccagg atcaccgacg ttccgaaatc
      181 gcgcgtgagc ccctttacca gctcgaggat ctgggcctgc accgtcacgt cgagcgccgt
      241 ggtcggttca tcggccagga ggagctcggg gcggcagaga agcgccatgg cgatcatcac
      301 ccgctggcgc atgccacccg agaactggtg gggatggtcg tcgatgcggc gcccggcgtc
      361 ggcaatcccg acgcgctgga gcatctcgat cgcgcgcctc cgcgcttcgc ggccggaaac
      421 gcgctcgtgg atttcaagca cctcggtcaa ctggcgggcc acggtaagga acgggttgag
      481 ggacgacatc gggtcctgga agaccatggc cacccggcgg ccgcgcacgg cccgcatttc
      541 cggctcggaa agatcgagca gattcacgcc cgagaggagg acctggcccc gttcgatgcg
      601 tccgggcgga tcggggatga ggcgcaggat cgagaggttc gtcacactct tcccgcagcc
      661 gctctcgccg accagcccca gcgtctcgcc ggcggccagc gcgaggtcga ggccgtccac
      721 cgcccgcacg gtgccgccgt cggtgcgaaa cgtcgtggcc aggtcccgga tctcgagaag
      781 gggcgagctc actctttcct caggtgggga tcgagcgcgt cgcgcaggcc gtctcccagg
      841 aagttgaggc agaagagcgt gagcgccagc gcgaaacccg gaaagatcac cagccagggg
      901 aagagcccca tgacgctggc cccctcgttc accagcacgc cccacgacgc atccggcggc
      961 tgcacgccga ggcccaggaa cgacagaaac gcctcctgca gcatgacggc cggcacggtg
     1021 agcgtggcgt agacgatgat cggccccagc gcgttcggga tgaggtgccg gaagatgatc
     1081 ccggcgtggc tcgtgccgat ggcccgcgcc gcttccacga actcgcggtg cttgagagag
     1141 agcacctgcc cgcggacgat gcgggccatg gtcagccact ggaccgcgcc gagggccacg
     1201 aagagcatga cgatgctccg gctgaagaag acgaggagca ggatgacgag gaacatgtac
     1261 ggcaggctgt agaggacatc cacgaagcgc atcatgaggt tgtcgagccg gccgccgaag
     1321 tagccggcca ccgcgcccca cgtcacgccg atcaggaacg acaccagcgt ggccacgagg
     1381 ccgacgagga ggctgatctg gccgccgtag agcacccgcg ccagcaagtc gcgtccgagg
     1441 atgtcggtgc ccattggatg cccggggctg gggggcaccg cgccaagttc caggtcctgc
     1501 cggttggggt cgggcacgcc cggcaggatc ggcgccgaca cgaccagtaa ggccacgacg
     1561 agcagtacga ggcccgagat caccgccatg cggttcttgc ggaggcgctt ccaggcatcg
     1621 ctccagaggc tggcgcccgg ctccagggcg atgggtacgg gcaccggggc ctcgcgcacc
     1681 gcgctcacga cagccgcacc cggggatcga gcaggccgta cgccacgtcc acgatcaggt
     1741 tgaagaggac gaggaacgtg gcgtagaaga gcaccacacc catcacgagc gtgtagtcgc
     1801 ggttgaaggc gccgtccacg aaatagcgcc cgaggccggg gatggcgaat atcttctcca
     1861 ccaccaccgc gccggtcacg atgcgggccg cggccggccc gaggaacgac accaccggca
     1921 gcaatcctcc gcgcaacgcg tgccgccaca cgataagcga ttcggaaagc cccttcgccc
     1981 gggccgtccg gatccagtcc tgccggatca cttcgagcat gccgctccgg gtgaggcgcg
     2041 cgatgtacgc cacgtacacc gaccccagcg tgatgaccgg caggatcttg gtggagggcc
     2101 cttcccataa cgccggcggc agccagtaga gtgagagcga aaagatgaag actagtagcg
     2161 gccccagcac gaaatccggg attgacacgc cgatgagcgc cgcggccatg gaggcatggt
     2221 ccaccggctt gttctgattg agcgccgcga tcacgccgat cgggatcccc acggccagcg
     2281 cgaacagcag cgcccagctc cccagctcca gcgacaccgg gaagcccgac gcaatgatct
     2341 cgcggacggt gcgaccgggc gtcttgtacg acgggccgaa ctccagcctc accacgttgc
     2401 cgaggaacgt gaggtattgc tcgcccagcg gcttgtcgag gccgtagcgc gccatcagcg
     2461 cttccttcac atcctcgggg atttccttct cggcctggaa cggcccgccg ggggcggccc
     2521 gcatcaggaa gaaggtgatc gtggccacca cccagagcgt ggggatcatc cagaggaggc
     2581 gccggaggat gaatcggatc atggggtggg cgtcttctcc cagttccggt cgatccaggc
     2641 gaacttgagg ttgtgcgtgt cggtgagatt cggttccacg ccttttacgt acggcttctt
     2701 gagctcggtg agcgtgtagt tgtagatcgg catcaccggg cactcgtcga ggaggatggc
     2761 ctcggccttc gccagcaccg cgtagcgggc cgccggatcg aacgtacggc tggcctcgag
     2821 cagcagcgcg tcgtacgcgg cgctcgacca gccggtccgg ttgtttccgt cacccgtctt
     2881 gtagcaatcg aggaaggtgg ccgggtcggc gtagtcgccg atccacgaac ggcgggccac
     2941 ctggtagttg aggccggtgg tgttgcgcag gtatgagccc cactcctggt tcgagagccc
     3001 cacgtcgatg ccgaggtggt ccttccacat ggcctggatg atctcggcga tcttccggtg
     3061 atcctccgac gtgttgaaca tgatctcgat cttcggcaat cccttgccgc ccgggtagcc
     3121 ggctttcgcc aggcactccc gcgcgaaagc cgggtcgaac ttctgaggcg cgggataggg
     3181 atagcccgcg aatccctgcg ccacaaagtt accatgcggg atcttcatcc ccttgaagag
     3241 ctggctgcag atggcctcgc gatccaccga ccaggagagc gcccgcctca gccacacgtt
     3301 tctcatccgg ccgtcggtcg tgttgaacga gtagaagtag acggccaggt aaggggcgta
     3361 gtggaagtcg gccgaatcct tcacgtacgg caggtactgg gccggcagat agccgctcgg
     3421 gttccagtcg gtggtgcccg ccttgtacag gttgagcgag gtgttcaggt cgtcgatggc
     3481 gtaggcccgg atgccgtcca gcttcacgtt ggccttgtcc cagtagcgct cgttccggac
     3541 gaattcgaaa cggttggcgg gcctccagaa ggtgcagcgg aagggtccgt tcaccacgac
     3601 gttttccggc cggctccatc gctcgccgaa tttctccacg caccaccgcg gcaccggcat
     3661 gtacgtgtag aaacaggtga gcgacaggaa gtagggcgtg gggttctcca gcgtgacgat
     3721 caacgtcgag tcgtcgggcg ccgcaatgcc gaccgagtcg acatcggcga tcgtcccctt
     3781 ctggtacgcc tcggcatt
//
LOCUS       DASVOD010000526         6986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_452711, whole genome shotgun sequence.
ACCESSION   DASVOD010000526 DASVOD010000000
VERSION     DASVOD010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6986
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452711"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF720_11715"
     CDS             <1..216
                     /locus_tag="VF720_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880071.1"
                     /translation="VAPAWLAVAAGAAGDLDLAIAYAERAVQEHDPLIVWSRRVPFWD
                     AMRRHPRFAEVTQVVWARTRQALGLAP"
     gene            213..1022
                     /locus_tag="VF720_11720"
     CDS             213..1022
                     /locus_tag="VF720_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009240246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880072.1"
                     /translation="MTTPRPLAGRPAIVTGAGRGIGLAIATELAALGASVALLGRTEE
                     KLREAAGQLTDAFDGTHTTAHGQHAIAIACDVTDDASVAAACAEAARRLGDIYLLVNN
                     AGTAPAAGILEITREEWDTAFDVNVTGAWRCVRCVVPGMITRGEGRIIQVASTAGLKG
                     YAGVSAYVAAKHALVGFTRALALELGPKGITVNAVCPGYTNTDIARQAIDNLVSARGL
                     TPDEARAKLTRHNPSGRLVEPHEVASVVGWLSLPESGSITGQAIAVAGGEI"
     gene            1022..1846
                     /locus_tag="VF720_11725"
     CDS             1022..1846
                     /locus_tag="VF720_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX7880073.1"
                     /translation="MTRGADFAPQHFRWSVADRVATITFDRPERKNPLTFDSYRELTD
                     TFDALGAVDDVRAVVLIGAGGNFCSGGDVNDIIGPLVEWKNAGRIDKLREFTHLTGGL
                     VKAMRGCPQPVVAAVDGMCVGAGAILAMASDIRFGTAGSKVGFLFVKVGLSGADMGAC
                     ALLPRIIGQGRASELLYTGRFMEGAEAERWGFYNALVEPAALLDHALGFARSLANGPA
                     FAHAMTKKCLHEEWAMGVDAAIAFEAEAQAICMKTNDFERAYRAFTEKRKPEFAGD"
     gene            2007..3218
                     /locus_tag="VF720_11730"
     CDS             2007..3218
                     /locus_tag="VF720_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7880074.1"
                     /translation="MADTTWLSWPFFDDSHRRLADELSSWCDREIAPVVSGEEHDLDS
                     ACRTYVKLLGAGGWLAYLMPVVHGGVHERPDLRSLCIIRETLGFHSPLADFCFALQGL
                     GTTPVVKLGTPEQQAAWLPRIREGDVVTAFAISEPEGGSDVAAMTTTARREGDDWIVD
                     GTKTWISNAGIADYYIVFARWPDGGEKSYVALLVEAGAPGLDTSERLEVMAPHPIGTI
                     KLHNCRVPGANLIGQPGKGLGVALATLDLFRSTVGAAALGYARRALDEAAAWSQERRT
                     YGKTLAEHQMTRGKLADMAVSVDAMALLVYRAGWVYDRGGQGRVTREAAMAKLYATEA
                     AWKVIDDAVQLFGGRGVLKGHPVERLYREIRALRIYEGASEIQKIIIANQILSPDAVR
                     AARAPSSDGNS"
     gene            3232..4875
                     /locus_tag="VF720_11735"
     CDS             3232..4875
                     /locus_tag="VF720_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX7880075.1"
                     /translation="MPPATERVATAFIDTFALDHLPPAEMQPEFDLAAVPHLAGRPRL
                     NAATEFLDGAVARGWGERRALVTDGETWSYARLLAESNRIGCALIEDYGLLPGQRVLL
                     RGPNSPRMAASWFGVLKAGGIAVTTMPQLRARELIYNMNKAKVSLALCDVRFAAELET
                     AAAATPSLNKVVHFGGPANPGALEGKAARKSDDFAGVDTAAEDIAIIAFTSGTTGKAK
                     GTMHHHRDLVAMCDLFPKSILKATADDLFCGSPPFAFTFGLGGILLFPLRIGAATLLL
                     ESAPPPLLAEGIARHRATVCFTAPTAYRAMLPLAAVHDLSSLTRCVSAGEHLPKATFE
                     AWKAATGLSIIDGIGSTEMLHIFISASGDELRPGSTGKAIPGYTAAILDDDGNELPAG
                     ELGRLAVRGVTGCRYLDDPERQAAYVQHGWNITGDTYRRDADGYFWYAARSDDMIISG
                     GYNISGVEVENVLLLHPAVAECGVVGVPDEDRGQVVAAYVVTKPGQVAGAELCKTLQE
                     FVKAEIAPYKYPRRIEFVNALPRTETGKLQRFRLRAEGA"
     gene            4872..5285
                     /locus_tag="VF720_11740"
     CDS             4872..5285
                     /locus_tag="VF720_11740"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX7880076.1"
                     /translation="MSGKDGEALLPEGWPRPVGYANGVSVPCGRFITVAGQVGWNPHT
                     LEFESSDLATQTRTALANVLAVLSAGGAEPRHVVRMTWYVTDRQAYLDARTAIGRAWR
                     ELFGSHYPAMSLVVVAGLLEALALVEIEATAVVPD"
     gene            5467..>6986
                     /locus_tag="VF720_11745"
     CDS             5467..>6986
                     /locus_tag="VF720_11745"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7880077.1"
                     /translation="MPIPSVSSLSRRGVFLSALVGLALLPAAVRAADKAEVVPFSPGP
                     YIPDIATFLNIGACSPAGYSWDGKTVYFTASMSGAGQVYRITDQGWPYQLTTFEDGIE
                     GFNLSWNGAMAIVSASVGGDENAQLYLLDTDTGRIEPLTQQPKVQFGSVTWIPDDSGI
                     YYRSNEENGKDFFIYRMDFPSRKVTKVYDKTEGYNSVAELSLDNTIMIVSNSTSNVNN
                     DLYVLDLKSGQSRQLTKDTGDVSYESVNLMPDKKTIWLTSNGNPDGISKLGTMSIDSP
                     EISWVNDGWLDPKWEVEGVSFSRDYKTMAVRQNEDGYGRLKMREVSSKKPLPNPPLDG
                     ILGSVLFDKDGACILSFSGPTRAPDVWKWNPRTKELKQLTYTIYAGIDRNLFKDPQLV
                     RYPTFDGRMIPAFMYLPPNYKPGTPVPFVVDAHGGPESQFRPDFIRNFQYLMLIGYGV
                     LAPNVRGSSGYGIEDLNLDNYKNRKDSLKDYKAGVDWLVKEGYTKPGMIGIRGGSYG"
BASE COUNT         1164 a         2375 c         2345 g         1102 t
ORIGIN      
        1 gtggccccgg cctggctggc ggtggcggcc ggcgcggccg gcgatctcga cctggccatc
       61 gcctacgccg agcgcgccgt gcaggagcac gaccccctga tcgtctggtc gcgtcgcgtt
      121 cctttctggg atgccatgcg ccggcatccg cggttcgccg aggtgacgca ggtggtgtgg
      181 gcgaggaccc ggcaggcgtt ggggctggca ccatgaccac tccgcgccct cttgccggtc
      241 gccccgccat cgtcaccggc gcgggacgcg gaatcggcct ggccatcgcc accgagctgg
      301 cggcgctcgg cgcctcggta gcccttcttg gccggacgga agaaaagctg cgcgaggcgg
      361 ccgggcagct gaccgacgcg ttcgacggca cgcacaccac cgcgcacggc cagcatgcca
      421 tcgccattgc ctgcgacgtg acggacgacg cctccgtggc cgccgcgtgc gccgaggcgg
      481 cccggcgcct cggcgacatc tacctcctcg tgaacaatgc cggcacggct ccggccgccg
      541 gcatcctcga gataacgcgc gaggaatggg acaccgcatt cgacgtgaac gtgaccggcg
      601 cctggcgctg cgtgcgctgc gtcgtacccg gcatgatcac ccggggagag ggacgcatca
      661 tccaggtggc atccaccgcg ggcctgaagg gatacgccgg cgtttcggcc tatgtggctg
      721 ccaagcatgc cctggtcgga ttcacgcggg cgctggcgct ggagcttggg ccgaagggga
      781 tcaccgtcaa cgcggtgtgc ccgggctaca cgaacacgga catcgcccgg caggccatcg
      841 acaacctggt ttcggcgcgg ggcctcacgc ccgacgaggc gcgtgcgaaa ctcacccggc
      901 acaatccgtc ggggcgactg gtcgagccgc acgaggtggc gtcggtggtg ggatggctca
      961 gccttcccga gtcgggatcg atcacgggac aggccatcgc agtggccgga ggagaaatct
     1021 gatgacgcgc ggggccgact tcgcaccgca acacttccgc tggtcggtgg ccgaccgggt
     1081 ggccacgatc acgttcgatc ggccggaacg gaagaacccg ctcaccttcg attcgtatcg
     1141 cgagctgacc gacacgttcg acgcgctggg cgccgtcgac gacgtgcgcg ccgtggtcct
     1201 catcggcgcg ggcggtaact tctgctccgg cggcgacgtc aacgacatca tcggcccgct
     1261 ggtggagtgg aaaaacgccg gccggatcga caagctccgc gagttcactc atctcaccgg
     1321 tggattggtc aaggcgatgc ggggctgccc ccagccggtc gtggccgcgg tggatggcat
     1381 gtgcgtcggc gcgggcgcca tcctggcgat ggccagcgac attcgctttg gcaccgcggg
     1441 tagcaaggtc ggcttcctct tcgtgaaagt cggcctgtcg ggcgccgaca tgggagcgtg
     1501 tgcgctcctg cctcgcatca tcgggcaggg gcgggcctcg gagttgctct ataccggccg
     1561 cttcatggag ggcgccgagg ccgagcgctg ggggttttac aatgcgctgg tggaacccgc
     1621 cgcgctgctc gaccacgccc tcggcttcgc gcggagcctc gccaacggcc cggccttcgc
     1681 gcacgccatg accaagaagt gcctgcacga ggagtgggcg atgggcgtgg atgccgccat
     1741 cgccttcgaa gccgaggccc aggccatctg catgaagacg aatgacttcg agcgggcgta
     1801 ccgcgccttc acggagaagc ggaaaccgga attcgcgggg gattgatgga gtggaatata
     1861 tttcgaaacc gtccaacacg attccatcac gacggttgcc gctcgtttga cgccgtcgcc
     1921 ctgccccaat acgatcgaaa cgtgcgtttt ccgcctacat tagataggca gtgcccgcgt
     1981 ccctcccggc tggagcccct tgcctgatgg ccgacaccac ctggctctcc tggcccttct
     2041 tcgacgactc ccaccgacgc ctggcggatg agctgagctc gtggtgcgat cgggagattg
     2101 cgccagttgt ctccggcgag gagcacgacc tcgactcggc ctgccggacc tacgtgaaac
     2161 tcctcggcgc cggcgggtgg ctggcctacc tcatgccggt tgtccacggc ggcgttcacg
     2221 agcggcccga cctccgcagc ctctgcatca ttcgcgaaac ccttggcttc cactcgccgc
     2281 tggcggattt ctgtttcgcg ctgcagggac tcggcacgac acccgtggtg aagctcggca
     2341 cgcccgagca acaggcggcc tggctgccac gcatccgaga gggtgatgtc gtcacggcct
     2401 tcgccatctc cgaacccgaa gggggatcgg acgtggcggc catgaccacg acggcgcggc
     2461 gcgagggtga cgactggatc gtggacggca cgaagacctg gatcagcaac gccggcatcg
     2521 ccgattacta cattgtcttc gcgcgatggc ccgacggggg cgagaagtcg tacgtggcgt
     2581 tgctcgtcga ggccggggcg ccgggactcg acacctcgga gcgcctcgag gtgatggcgc
     2641 cccaccccat cggcacgatc aagctgcaca actgccgcgt accgggcgcc aatctcatcg
     2701 gtcagccggg caagggactc ggggtggcgc tggccacgct cgatctcttc cgctctacgg
     2761 tgggcgcggc ggcattgggc tacgcccgcc gggcgctgga tgaagccgcc gcctggagcc
     2821 aggaacgccg cacctacggc aagacgctgg ccgagcacca gatgacgcgc gggaaactgg
     2881 ccgacatggc cgtctccgtc gatgccatgg cgctcctggt gtatcgcgcc gggtgggtct
     2941 acgatcgcgg ggggcaggga cgggtgaccc gtgaggcggc catggccaag ctctacgcca
     3001 ccgaggcggc ctggaaggtg atcgacgacg cggtgcagct cttcggcggc cgcggcgtgc
     3061 tgaaaggcca tcccgtcgag cgcctctacc gggagatccg ggcgcttcgc atctacgaag
     3121 gcgcctcgga aatccagaag atcatcattg ccaaccagat tttgagcccg gacgcggttc
     3181 gtgccgcacg ggcgccgtcg agcgacggaa actcctgact tgggagacgg aatgcctcct
     3241 gccaccgagc gcgtcgccac cgcgttcatc gacacgttcg cgctggacca cctgccgccg
     3301 gcggagatgc agccggaatt cgacctcgcg gccgtcccgc acctggctgg ccgccctcgg
     3361 ctcaacgcgg cgacggaatt tctggacggg gcggtggccc ggggctgggg agaacgtcgc
     3421 gcgctggtca cggatggcga gacgtggagc tacgcgcgcc tgctggccga atcgaaccgc
     3481 atcggctgcg cgttgatcga ggactacggc ctgctgccgg ggcagcgcgt cctgttgcgc
     3541 ggcccgaact cgccgaggat ggccgcctcg tggttcggcg tgctcaaggc cggcggcatt
     3601 gcggtgacga cgatgccgca acttcgcgcc cgcgagctga tctacaacat gaacaaggcg
     3661 aaggtgagcc tggccctgtg cgacgtgcgc tttgccgccg agctcgagac ggccgcagcg
     3721 gccacgccgt cgctgaacaa ggtggtgcac ttcggcggcc ccgccaatcc cggcgcgctg
     3781 gaagggaagg cggcccgcaa gagcgacgac ttcgcgggcg tcgacacggc ggccgaggac
     3841 atcgccatca tcgccttcac ctcgggcacg accggcaagg ccaaggggac gatgcaccac
     3901 caccgcgacc tggtggccat gtgcgatctc ttcccgaagt cgatcctgaa ggccacggcc
     3961 gacgacctgt tctgcggctc accgccgttc gcgttcacgt tcggccttgg cggcatcctc
     4021 ctctttccgc tccggatcgg ggcggccacg ttgctgctgg agtcggcgcc gccgccgctc
     4081 ctggccgagg ggattgcgcg gcaccgggcc actgtgtgct tcacggcgcc cacggcctac
     4141 cgcgccatgc tcccgctggc cgccgtccac gacctgtcga gcctgacacg atgcgtgtcg
     4201 gcgggggaac atctcccgaa ggccaccttc gaggcatgga aggccgccac cggactgtcg
     4261 atcatcgacg gcatcggctc gaccgagatg ctccacatct tcatcagcgc ttcaggggat
     4321 gagctgcggc cgggctcgac cggcaaggcc atacccggtt acaccgccgc gatcctcgac
     4381 gatgacggca acgagttgcc ggccggcgaa ctggggcgcc tggcggtgcg cggggtgacc
     4441 ggctgccgct acctggatga tcccgagcga caggcggcct acgtgcagca tggctggaac
     4501 atcaccggcg atacctaccg ccgcgatgcc gacggctact tctggtacgc cgcgcggagc
     4561 gacgacatga tcatttccgg tggatacaac atctcggggg tggaagtgga aaacgtgctg
     4621 ctgttgcatc cggccgtggc cgagtgcggc gtggtcggcg tgccggacga ggatcggggc
     4681 caggtggtcg ccgcctacgt ggtgacgaag ccgggccagg tagccggcgc cgagctgtgc
     4741 aagacgctcc aggaattcgt gaaggccgag atcgcgccgt acaagtatcc gcggcggatc
     4801 gaattcgtaa acgccctgcc acgcacggaa accggcaagc tgcagcggtt tcggctgcgc
     4861 gcggagggag cgtgagcggc aaggacggcg aggcgctcct acccgaaggg tggccgcggc
     4921 cggtcggcta cgccaacggc gtcagcgtgc cgtgcgggcg gttcatcacc gtggccggcc
     4981 aggtcgggtg gaatccgcac acgctcgaat ttgaatcgag cgacctcgcc acgcagaccc
     5041 gcaccgcgct ggccaacgtg ctggcggtgc tctccgccgg cggagccgag ccgcgacacg
     5101 tcgtgaggat gacctggtac gtcaccgacc ggcaggccta cctcgacgcg cgcaccgcca
     5161 tcggccgggc ctggcgcgag ctgttcggga gccactaccc ggccatgtcg ctggtggtgg
     5221 tggcggggct tctcgaggcc cttgcactgg tggaaatcga agccacggcg gtcgttccgg
     5281 actgaacgga acggcgctcc gttccgtctg acgcctggat tcgtccgacc cggccccacg
     5341 gccggattcg ggtggacggt gccggaggcg ccccctacaa tgacacgccg ccgcgccgcc
     5401 accggcccgg acgtttcatt cgccgatcat tcatcgggtt accctgcccc cgcgaggaga
     5461 ccaaccgtgc ccattccatc cgtttcgtcg ctcagccgcc gcggcgtatt cctgtccgcg
     5521 ctggtcggac ttgcgctgtt gccggcggct gtccgtgccg ccgacaaggc cgaggtggtg
     5581 cctttcagcc cgggccccta catccccgac atcgccacct tcctgaacat cggcgcctgc
     5641 tccccggccg gttacagctg ggatgggaag acggtctact tcacggccag catgtccggt
     5701 gcgggacagg tgtaccgcat caccgaccag gggtggccgt accagctcac cacctttgag
     5761 gacggcatcg aggggttcaa cctctcctgg aacggcgcga tggccatcgt cagcgcctcg
     5821 gtcgggggcg acgaaaacgc ccagctctac ctcctcgaca ccgacaccgg acgcatcgag
     5881 ccgctcacgc agcagccgaa agtgcagttc gggtcggtca cctggatccc ggacgactcg
     5941 gggatctact accgctcgaa cgaggagaac ggcaaggact tcttcatcta ccggatggac
     6001 tttccgagcc ggaaggtcac caaggtgtac gacaagaccg aggggtacaa ctcggtggcc
     6061 gagctctccc tcgacaacac catcatgatc gtcagcaatt ccacgtcgaa cgtgaacaac
     6121 gacctctacg tgctcgacct gaagagcggc cagtcgcggc agctcacgaa ggataccggc
     6181 gacgtgtcgt acgagtcggt caacctcatg cccgacaaga agacgatctg gctcaccagc
     6241 aacggcaatc cggacggcat cagcaagctc ggcacgatgt cgatcgactc gcccgagatc
     6301 tcctgggtga acgatggctg gctcgatccc aagtgggaag tggagggtgt ctccttcagc
     6361 cgtgactaca agacgatggc cgtccgccag aacgaggatg gctacgggcg cctcaagatg
     6421 cgcgaggtgt cctcgaagaa gccgctgccg aacccgcccc tcgacggtat cctggggagc
     6481 gtcctcttcg acaaggacgg cgcctgcatc ctttcgttct ccggccccac gcgcgcaccc
     6541 gacgtctgga agtggaatcc gcgcacgaag gagctcaagc agctcaccta cacgatctac
     6601 gccggcatcg accgcaatct cttcaaggat ccgcagctcg tccgctaccc gacgttcgac
     6661 ggcaggatga tcccggcgtt catgtacctg ccgcccaact acaagcccgg cacgcccgtt
     6721 ccgttcgtgg tggatgcgca cggcggcccc gagagccagt tccggcccga cttcatccgc
     6781 aacttccagt atctgatgct catcggctac ggcgtgctcg cacccaacgt gcgcggctcg
     6841 tccggctacg gcatcgaaga tctcaacctc gacaactaca agaaccgcaa ggacagcctc
     6901 aaggactaca aggccggcgt ggactggctg gtgaaggagg gctataccaa gcccggcatg
     6961 atcggcatcc gcggcggctc ctacgg
//
LOCUS       DASVOD010000527         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_296173, whole genome shotgun sequence.
ACCESSION   DASVOD010000527 DASVOD010000000
VERSION     DASVOD010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296173"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /gene="hutU"
                     /locus_tag="VF720_11750"
     CDS             complement(<1..1535)
                     /gene="hutU"
                     /locus_tag="VF720_11750"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX7880078.1"
                     /translation="MTTTLAARAVRAPRGTTRTCRSWLTEAAMRMIMNNLDPDVAERP
                     EELIVYGGTGKAARSWDDFDRIVATLKRLDDDETLLVQSGRPVGVVTTHADAPRVLIA
                     NSNLVGKWATWEHFRELEKKGLMMYGQMTAGSWIYIGSQGIVQGTFETLAEAARRHFG
                     ADLAGRLTVTAGLGGMGGAQPLAVTMNGGVCLAAEVDRTRLAKRLETRYLDREAPDLE
                     SALTMAREAMAAREPLSIGVVMNAADMMDELVKRNVTPDLLTDQTSAHDPLNGYVPRG
                     MTLEAATALRVSNPDDYVRRSRATMADHVRAMLTLQKRGAHTFDYGNNIRGEAQLGGV
                     ADAFDIPGFVPEYIRPLFCRGKGPFRWVALSGDPADIAVTDAALLAEFPDDPILRRWL
                     PLAAERIAFQGLPARICWLGYGERHRAGRLFNELVRTGKVSAPIVIGRDHLDAGSVAS
                     PYRETEAMKDGTDAVADWPILNALVNTASGATWVSFHHGGGVGIGNSLHAGQVIVADG
                     TPEA"
     gene            complement(1532..3064)
                     /gene="hutH"
                     /locus_tag="VF720_11755"
     CDS             complement(1532..3064)
                     /gene="hutH"
                     /locus_tag="VF720_11755"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX7880079.1"
                     /translation="MSDPVRLDHGALTVADLEGVARQGRTVVIGEAARAAMVRSRRLV
                     EDTLTAGRVVYGINTGFGELSHVTIPKEDVARLQLNLVRSHSVGVGHPLAEDVVRGML
                     LLRADSLSRGYSGVRPEVVELLVGMLNAGVMPVVPEQGSVGASGDLAPLAHLAVVLVG
                     EGEATHGGRRMPGNEALRAAGLKPVTLAAKEGLALINGTQAMTSSAALAIADAERLLD
                     GAQAAAALCLDALKGSVVPFDPRFHAVRPHPGAGQVARNLRRLLQASAIADSHVGCAK
                     VQDPYSIRCAAVVLGASHDALVHVREVVQREMRAVTDNPLCFADAGEILSGGNFHGQP
                     IALVLDYLAIAMAEVASISERRTYLVLDGHLNGLTPFLARTPGLQSGLMMAQYTAAAL
                     VSENKVLAHPASVDSIPTSAGMEDHVSMGMTGAVKLRRILDNVRRVLAVELISGAEGL
                     ERHRPLTSGAGAEEVVTRVRRHVAPLTDDRVLSGDIESISEAIRRGEFTLASLLNEGT
                     EP"
     gene            complement(3061..5172)
                     /locus_tag="VF720_11760"
     CDS             complement(3061..5172)
                     /locus_tag="VF720_11760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX7880080.1"
                     /translation="MPFTTTELLSVFEPGGPLSHEIEGFEPRRQQVEMAREVALVLEH
                     GGRLVVEAPTGVGKSLAYLLPSLAWARSAGVPVVVATFTRALQDQLRNADAPIALAVL
                     NSRGTVAVLKGRANYLCRRRLGRAMSAAPPGQQKVFERLMDWADRTSSGDFAECDTLG
                     AEELALIAPRVASDARACAGNLCSPAEGCFFKRARALAAQADVIIVNHALLTIELLGE
                     KGMLPDHGALVVDEAHHLEDAATRQMTISVGGRRMKAAGENVQATGGDGAGAVVKKLL
                     TAWRNDEADPVRAVLADWQKAAGTAGARGEVWFGRLNAGPGPVGGDLRQRYTDAAGLA
                     AMAPGETGELTRSVDETVRLGARLLYELNEAATQSPDPDETRAAAAEVESALSEWKGI
                     QEALDITLHPEGAGSEWVHWREWGDREHWNLVAAPRDVSGPLGQRLRERQERIVLTSA
                     TLTVGGHFDHILRRLGLPIDAATLALDTPFDFASSVRVFSVANVPDPRDADYGRHLAR
                     TIEELVHATKRKSLALFTSYAMLREVTPQLAALEDAGVRVLAQGKDGSVASILRQFKK
                     PGPALVLGTASFWEGIDLPGELLEILVVTRLPFPVPSDPIVEARGDELKLAGHDPFMS
                     YSVPEAVLKLKQGFGRLIRRQGDKGVVAILDGRFLTARYGGLFRSALPVAPERCTDAH
                     DLAGRARDWMAADPVVAGEAS"
BASE COUNT          810 a         1872 c         1773 g          785 t
ORIGIN      
        1 gcttcgggcg tgccgtcggc cacgatcacc tggccggcgt ggagcgaatt gccgatgccc
       61 acgccgccgc cgtggtggaa cgacacccag gtggcgcccg aggccgtgtt gaccagcgcg
      121 ttcaggatcg gccagtcggc caccgcgtcg gtgccgtcct tcatcgcctc ggtttcccga
      181 taggggctgg ccacgctccc ggcgtcgagg tgatcccggc cgatgacgat cggcgccgac
      241 acctttccgg tacggacgag ttcattgaag agccgcccgg cccggtggcg ctcgccgtaa
      301 cccagccagc agattcgcgc cggcaatccc tggaaggcga tccgctcggc ggccagcggc
      361 aaccagcgtc tcaggatcgg atcgtcgggg aattcagcga gaagcgcggc atccgtcacg
      421 gcgatgtcgg ccggatcgcc cgacagggcc acccagcgga acggcccctt tccccggcag
      481 aagagaggac ggatgtattc cggcacgaag ccggggatat cgaaggcatc ggccacgcca
      541 ccgagctgcg cctcgccacg gatgttgttg ccgtaatcga aggtgtgggc gccccgtttc
      601 tggagcgtga gcatcgcgcg gacatgatcg gccatggtgg cgcgcgaccg gcggacgtag
      661 tcgtcgggat tcgagacgcg cagggcagtg gccgcctcga gcgtcatgcc gcgcggcaca
      721 tagccattca acggatcgtg cgcgctcgtc tggtcggtaa ggaggtcggg cgtgacgttc
      781 cgcttcacga gctcatccat catgtcggcc gcattcatca cgacaccgat cgacagcggc
      841 tccctggcgg ccatcgcctc gcgcgccatc gttaaagcgc tctcgagatc gggcgcctcg
      901 cggtcgagat agcgcgtttc cagccgcttc gcgagccgcg tgcgatcgac ctcggccgcg
      961 aggcagacgc cgccgttcat cgtgacggcc agcggctggg cgccccccat cccgccgagg
     1021 ccggcggtga cggtgagccg tcctgccagg tccgcgccga agtgacgacg cgccgcttcg
     1081 gccagcgtct cgaacgtccc ctgcacgatc ccctggctgc cgatgtagat ccacgatccg
     1141 gccgtcatct ggccgtacat catgagtccc ttcttctcca gctcgcggaa gtgctcccag
     1201 gtggcccact tgccgaccag gttcgaattg gcgatgagca cccgcggcgc gtcggcatgc
     1261 gtggtgacga cgcccaccgg gcggccggac tggacgagga gcgtctcgtc atcgtcgagg
     1321 cgcttcagcg tggccacgat ccggtcgaag tcgtcccagc tccgggccgc cttgccggtg
     1381 ccgccgtaca cgatcaactc ctccgggcgc tcggccacgt cgggatcgag gttgttcatg
     1441 atcatccgca tggccgcttc ggtgagccag gagcggcagg tgcgggtggt gccgcgcggg
     1501 gcgcgaacgg cgcgggcggc cagggtcgtg gtcatggttc ggtcccttca ttgaggagcg
     1561 aggccagcgt gaactcgccc cgccggatcg cttcgctgat cgattcgatg tcgccggaga
     1621 ggacgcggtc gtcggtgagc ggggccacgt gacggcgaac ccgcgtcacc acttcctcgg
     1681 cgcccgctcc gctggtcagg gggcgatgcc tctccagtcc ctcggcaccg gagatcagtt
     1741 ccaccgccag gacgcgccgc acgttgtcga ggatgcgccg cagcttgacc gcgccggtca
     1801 tccccatgct cacatggtct tccatgccgg ccgaggtggg aatggaatcg acactggccg
     1861 gatgcgcgag caccttgttc tcggacacca gggcggcggc cgtgtactgg gccatcatga
     1921 ggcccgattg cagccccggc gtgcgcgcga ggaacggcgt caggccgttc aggtgaccat
     1981 cgagcacgag ataggtccgc cgctcgctga tcgacgccac ctcggccatg gcgatggcca
     2041 ggtagtcgag caccagggcg atcggctgtc cgtggaagtt gccgccggag aggatctccc
     2101 ccgcgtcggc gaagcagagc gggttgtcgg tgaccgcccg catctcccgc tgcacgacct
     2161 cacgcacatg gacgagcgca tcgtggctgg cgccgagcac gacggccgcg cagcgaatcg
     2221 agtagggatc ctgcaccttg gcgcagccga catggctgtc ggcgatcgcg ctggcctgca
     2281 gcaaccggcg caggttccgc gccacctgcc cggcgccggg atgcggccgc accgcgtgga
     2341 agcggggatc gaacggcacc acgctcccct tgagcgcgtc gaggcagagc gcggcggcgg
     2401 cctgcgcgcc gtcgagcagc cgttccgcgt cggcgatggc cagcgccgcg gaggaggtca
     2461 tggcctgggt gccgttgatg agcgccagcc cctccttggc ggccagcgtc accggtttga
     2521 gtccggccgc gcgcaaggcc tcgttgcccg gcatccgacg accgccgtga gtggcctctc
     2581 cctcccccac caggacgacg gcgagatggg ccagcggcgc gagatcgccg ctggcgccca
     2641 ccgaaccctg ttcggggacg accggcatga caccggcgtt cagcatgccg accagaagtt
     2701 cgacgacttc gggccggacg cccgaataac cgcgcgagag ggaatccgcg cggagcagga
     2761 gcatgccgcg caccacgtcc tcggcgaggg ggtgccccac gccgacggag tggctgcgaa
     2821 ccaggttcag ctgcaggcgc gccacgtcct ccttcggaat cgtgacgtgg gagagttcgc
     2881 cgaaaccggt gttgatgccg tagaccaccc ggcctgccgt cagcgtgtcc tcgacgagcc
     2941 ggcgagaacg gaccatcgcc gcccgggcgg cctcgccgat gacgaccgta cgcccctggc
     3001 gggccacccc ctcgagatcg gccaccgtga gtgcgccgtg atcgagccgg accggatcgc
     3061 tcacgatgcc tcccccgcca ccaccggatc cgccgccatc cagtcgcggg cgcggccggc
     3121 caggtcgtgc gcgtcggtgc agcgctcggg cgccaccggg agcgccgagc ggaagagtcc
     3181 gccgtaccgg gccgtcagga agcggccgtc gaggatggcc acgactccct tgtcaccctg
     3241 gcgtcgaatc agacgcccga aaccctgttt caatttcaat accgcctccg gcaccgaata
     3301 gctcatgaac ggatcgtgcc ccgcgagttt cagctcgtca ccgcgcgcct cgacgatcgg
     3361 atcgctcggc accgggaacg ggaggcgcgt cacgaccagg atttcgagaa gctcgcccgg
     3421 aaggtcgatt ccctcccaga acgacgccgt gccgagcacc agcgccggcc ccggcttctt
     3481 gaactgccgg aggatcgagg ccaccgagcc gtccttcccc tgggccagga cgcgcacgcc
     3541 ggcatcctcg agcgccgcca gttgtggcgt gacctcacgc agcatggcat acgaggtgaa
     3601 caacgccagc gacttccgct tcgtggcatg caccagttcc tcgatggtgc gggccaggtg
     3661 ccggccgtaa tcggcatcgc gcggatcggg gacgttggcc accgagaaga cccgcaccga
     3721 ggaagcgaag tcgaacggtg tatcgagggc cagcgtggcc gcatcgatcg gcaggccgag
     3781 ccgacgaagg atgtgatcga agtgtccgcc gaccgtgagt gtggccgagg tcagcacgat
     3841 gcgctcctgc cgctcgcgca gccgctggcc gagtgggccc gacacgtccc gcggggccgc
     3901 caccaggttc cagtgctcgc gatcccccca ctcgcgccag tgcacccact cgctccccgc
     3961 gccttcggga tgaagcgtga tgtcgagcgc ctcctggatt cccttccatt cggacaatgc
     4021 ggattccact tccgcggcgg cggcccgcgt ctcgtccgga tcgggtgatt gcgtggccgc
     4081 ctcgttgagt tcgtacagga gtcgcgcgcc gagtcgcacc gtctcgtcca cgctgcgggt
     4141 gagctcgccg gtttcacccg gcgccatcgc ggcgaggccg gccgcgtcgg tgtaacgctg
     4201 gcgcaggtcg ccacccaccg gacccggccc ggcattcaat cgaccgaacc acacctcgcc
     4261 acgggccccc gcggtgccgg ccgccttctg ccagtcggcg aggaccgcac gcaccggatc
     4321 ggcctcgtca ttccgccagg cggtgaggag cttcttcacc accgcgccgg caccatcgcc
     4381 gccggtggcc tggacattct cgccggccgc cttcatccgg cgccctccca ccgagatcgt
     4441 catctggcgc gtggcggcat cctcgagatg atgggcctcg tcgaccacca gcgccccatg
     4501 gtccggcagc atccccttct cgccgagcag ttcgatcgtg agaagcgcgt ggttgacgat
     4561 gatcacgtcg gcctgcgccg ccagcgcccg ggccctcttg aagaagcacc cttcggccgg
     4621 cgaacagagg ttgcccgcgc aggcgcgggc atccgatgcc acgcgcggcg cgatcagcgc
     4681 cagctcctcg gccccgagag tgtcgcactc ggcgaagtcg ccggaggacg tacgatcggc
     4741 ccagtccatc agccgctcga acaccttctg ctggcctggc ggcgcggcgc tcatggcccg
     4801 gccaagccgg cgccggcaga gatagttggc gcgccccttg agcaccgcca ccgtcccccg
     4861 cgaattgagg accgccaggg cgatcggcgc gtcggcgttt cgcaactgat cctgtaatgc
     4921 gcgcgtgaac gtggccacca cgaccggaac gccggccgac cgcgcccagg ccagggacgg
     4981 cagcaggtag gccagcgact tcccgacacc ggtcggcgcc tccacgacga gccgcccccc
     5041 atgctccagc acgagcgcca cctcgcgggc catctccacc tgctgcctgc ggggctcgaa
     5101 cccctcgatc tcgtggctga gaggcccccc gggctcgaat accgacaaca attcggtggt
     5161 ggtgaagggc atgcatcgga ccccggggga ggttacggct gtggttcgac gaaaggatga
     5221 ggcattatag gtaggttgtt
//
LOCUS       DASVOD010000528         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_869207, whole genome shotgun sequence.
ACCESSION   DASVOD010000528 DASVOD010000000
VERSION     DASVOD010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869207"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /gene="rimM"
                     /locus_tag="VF720_11765"
     CDS             <1..373
                     /gene="rimM"
                     /locus_tag="VF720_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX7880081.1"
                     /translation="VDCRIHRGAALVRFEGVTDRDRAEELVGGTLAIPREELLPAPEG
                     TIYVADLIGLAVVTAGGEPVGTLTAVYPGAGHDVYGVEREGREVLIPAVEPIVVELDM
                     DARRLVIDPPDGLLDIGVDRK"
     gene            370..1146
                     /gene="trmD"
                     /locus_tag="VF720_11770"
     CDS             370..1146
                     /gene="trmD"
                     /locus_tag="VF720_11770"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX7880082.1"
                     /translation="MNVGILTVFPKFFDGPLGEGMIRIARRDGKLNVQVVDLRDHTDD
                     AHRTTDDTMYGGGAGMVMKADPIVRAWEGLPPEYRRRTLLTSARGRRFDEALAQELAD
                     AEGFTILCGRYKGVDERVIALTGAEEVSIGDYVLSGGEPAALVMVDAAVRHVPGVLGD
                     YDSAVDDSHYEGLLGYPVYTRPEEYRGLSVPEVLLSGHHARIQAWRREEMLKATRTRR
                     PDLLDGKAMSAEDRKVLARLQADEGRADSGSKDPGKGTTS"
     gene            1143..1535
                     /gene="rplS"
                     /locus_tag="VF720_11775"
     CDS             1143..1535
                     /gene="rplS"
                     /locus_tag="VF720_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009554678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEX7880083.1"
                     /translation="MNRQQMIEKVERTQIRDIGTTPRPGDTVKVHVKVIEGDKSRIQL
                     FQGVIINVRGEGARKTFTVRKVSMGVAVERIFPFYSPSVDKVELVRTGKVRRAKLFYL
                     RGKKGKAARIAEGDDEKPQAAEKAAASE"
     gene            1691..>1887
                     /locus_tag="VF720_11780"
     CDS             1691..>1887
                     /locus_tag="VF720_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX7880084.1"
                     /translation="MSTVERRVRGDLLGFDEELRRRHGIVHLAGVDEVGRGPLAGPVI
                     AAAVILPAGVDVVEADDSKEL"
BASE COUNT          322 a          584 c          694 g          287 t
ORIGIN      
        1 ggtcgactgt cgcatccatc ggggcgcggc actggtccgg ttcgagggag ttacggatcg
       61 cgatcgggcc gaggaactgg tcggcggaac gctggccatt ccacgggagg agctccttcc
      121 ggcgcccgaa ggcaccatct acgtggccga cctgatcggc ctcgcggtgg tcacggcggg
      181 cggggagccg gtgggcacgc tcacggcggt ctatccgggc gccgggcacg atgtgtacgg
      241 cgtggagagg gaaggccggg aggtgttgat cccggcagtc gagccgatcg tggtcgagct
      301 cgacatggac gcccggcggc tcgtgatcga cccgccggac ggactgctcg atatcggggt
      361 ggatcggaag tgaacgtcgg catcctgacg gtctttccga agttcttcga cggaccgctc
      421 ggcgagggaa tgatccggat cgcccggcgg gacgggaaat tgaacgtcca ggtggtcgac
      481 cttcgcgacc acacggacga tgcgcatcgg acgaccgacg acacgatgta cggcggaggc
      541 gccggcatgg tgatgaaggc cgatccgatc gtgagggcct gggaggggct gccgccggaa
      601 tatcgccggc ggacgctgct gacttcggcc cggggccggc ggttcgatga agcgctggca
      661 caggaactgg cggacgcgga aggcttcacc atcctctgcg gccgctacaa gggtgtcgac
      721 gagcgcgtga tcgcgttgac cggcgccgag gaagtttcga tcggggatta cgtactttcg
      781 ggcggcgaac cggccgccct ggtcatggtc gacgcggcgg tccggcatgt gccgggcgtc
      841 ctcggcgatt acgacagcgc cgtggacgac tcgcactacg agggtctcct cggttacccg
      901 gtgtacacgc ggcccgagga gtatcgcggg ctttcggtgc ccgaggtgct gctgtcgggg
      961 caccacgccc ggattcaggc gtggcgccgc gaagagatgt tgaaggcaac gcgtacgcgc
     1021 cgccccgacc tgttggacgg gaaggccatg agcgcggagg atcggaaggt gctggcccgt
     1081 ctccaggcgg acgaggggcg ggccgatagc ggatcgaagg atccagggaa aggaaccacg
     1141 tcatgaatcg ccagcagatg atcgaaaaag tcgagcggac ccagatccgc gacatcggca
     1201 ccacgccccg acccggcgac accgtcaagg tgcacgtgaa ggtcatcgaa ggcgacaaga
     1261 gccgtatcca gctcttccag ggcgtcatca tcaacgtccg cggcgagggc gcccgcaaga
     1321 cgttcaccgt ccgcaaggtc tccatgggtg tggccgtcga gcgcatcttc ccgttctaca
     1381 gcccctcggt cgacaaggtc gagctcgtcc gcaccggcaa ggtccgccgc gccaagctct
     1441 tctacctccg cggcaagaag gggaaggcgg cccgcatcgc cgaaggcgac gacgagaagc
     1501 cgcaggccgc cgagaaggcc gccgcctccg agtaacagag ccggcgccgg cgtcaccgcc
     1561 cgccgggcac cgcgataccg atcctcacgt gcgtcccgtt ccgacggggc gcacgttttt
     1621 ttgggcgatt cgattttccc cgtagacatc tcgaaagaac ctcgtccaga cttcgcgccg
     1681 gagggcggag atgtcgacgg tcgagcgccg cgttcgcggc gacctgttgg ggttcgacga
     1741 ggaactgcgg cggcggcacg gcatcgtgca cctggccggc gtggacgagg tgggtcgggg
     1801 tccgttggcg gggccggtca tcgcggcggc ggtcatcctt cccgcgggtg tggatgtggt
     1861 cgaagccgac gactccaagg agctcga
//
LOCUS       DASVOD010000529         4173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_974863, whole genome shotgun sequence.
ACCESSION   DASVOD010000529 DASVOD010000000
VERSION     DASVOD010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4173
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974863"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF720_11785"
     CDS             complement(<1..643)
                     /locus_tag="VF720_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880085.1"
                     /translation="MTQRRLAAVRSALLALGLLASGLANAQEDKALTPNAHFMSGKLY
                     LSQKVFDKAEHEFAAAVEGDTANAEYRTYWATSLCEVAVMNLGSASSISDAAERRTAL
                     KAISDQLAEASRQFDRAGAMDPRKQASAAEDNRQHYWVDIYKQGLALIEAKRYEDAID
                     VFGLTNTLDPKDPSGVFQVAYANRQLGNTGEAVATAKRAKEMAQARLGELGDCS"
     gene            complement(1051..1866)
                     /locus_tag="VF720_11790"
     CDS             complement(1051..1866)
                     /locus_tag="VF720_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX7880086.1"
                     /translation="MTASSAKSIGGPILIGTSGYSFDDWEGPFYPQGTPKGKRLDFYV
                     QHFPCVEINSTYYRIPHPAVMMKLVEKTPPGFEFTVKANEAMTHRRSRDPETYRQFLE
                     CLDPMRREDRFHGVVLQFPWAFRNDASSRAHLAWLRGALPSMPLWAEFRHVSWDRPEV
                     YRFLGDLDLGFIAVDEPALPGLFPPVAEVTSEVAYVRFHGRNAASWWGREGHDRYDWD
                     YRRSELEEWVEKIRSMAARAKKTYVFFNNCYMGRAVTSARLMKGLLAGGGAPA"
     gene            complement(1863..3047)
                     /gene="dinB"
                     /locus_tag="VF720_11795"
     CDS             complement(1863..3047)
                     /gene="dinB"
                     /locus_tag="VF720_11795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX7880087.1"
                     /translation="MTGPIAHVDMDAFFAAVEVRANPKLEGKPLLVGGGPAGRQVVTT
                     ASYPARRFGIRSGMSLSEARSRCPAAIFLPVEPARYLSASEELVRLFERFTPLVEPAS
                     IDEVFLDLTGLAAEADGGEGLARAIQDSVRREQRLGCSIGLGETKLIAKMATSLHKPE
                     GLTRLTRADFQQVFWPRPTGALWGVGPESEAVLHQLGIRTIGELAACPTARLERVFGV
                     TAAVLVRMAAGEGGGSVVPYFEGLPVRSMGHETTFPVDLVEGKRLERHLLLLTDKVSR
                     RLRREGRSGHRVTLRIRFSDGTTITRQRALAAPTDDDRILFRVAAALLADNVGGRAVR
                     LLGINVGHLVGPGATGFLLPEDQRQQRLGEVRDRLRDRFGESSLIPGGVVALIKDEGQ
                     RS"
     gene            3215..>4173
                     /locus_tag="VF720_11800"
     CDS             3215..>4173
                     /locus_tag="VF720_11800"
                     /inference="COORDINATES: protein motif:HMM:NF016593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX7880088.1"
                     /translation="MATPTPQPTVLTRSISLKKTPAEVFARFSQQPGCFFLDSALGDH
                     ELSRFSYIGAEPFFTLKSRGMVMDIVEGKKVRHQKGNPLEVLASMLQRHQVSSGTTLV
                     PFIGGAVGYIAYEFGKHTTGLDLTAPDDLGLPELAFNFYRKLLAYDHRDRRWFVAAAD
                     FSTSHGPSVRKRLTADIDRMAAMADGSEPAAPPPPKVAADAAVDASEDDLAVVPESPM
                     VTDGPYPWRSSLTREQYLGAVRAIQERIGRGDIYQANLTQRFHATIPSSPVRLYQALR
                     EVNPAPYGCYLDLGECVIAGQSPELFLSIRGRHVETRPIKGTRP"
BASE COUNT          739 a         1376 c         1331 g          727 t
ORIGIN      
        1 ggctgcaatc cccgagctct ccgaggcggg cctgtgccat ctccttggca cgcttggcgg
       61 tggccacggc ctcgccggta tttccgagct gccggttggc gtacgccacc tggaagactc
      121 ccgagggatc cttcgggtcg agcgtattgg tcaggccgaa tacgtcgatc gcatcctcgt
      181 aacgcttggc ctcgatgaga gccagcccct gcttgtagat atccacccag tagtgctggc
      241 ggttgtcttc ggcggccgag gcctgcttcc tgggatccat cgcgccggcc cgatcgaact
      301 ggcgggacgc ttcggcgagc tggtcgctga tggccttcag cgccgtccgc cgctcggcgg
      361 cgtcggagat cgaggacgcg ctgccgagat tcatgaccgc cacctcgcag agcgaggtgg
      421 cccagtacgt acggtactcg gcgttcgccg tatcaccctc gacggccgcg gcgaattcgt
      481 gctcggcttt atcgaaaact ttctggctca gatagagttt gccgctcatg aagtgagcgt
      541 tgggtgtcag cgccttgtct tcctgggcgt tggcgagccc ggaggccagg agccccaggg
      601 cgaggagggc ggagcgaacg gcggccaaac gtcgttgggt catgtcggta cctctcaaaa
      661 gtcgagacgc gagccagttg cccggaatcg cgctccggtc atcggaacca cgcgcaagta
      721 tggaatctga cacaagttgg gcggccagcc cgaaggggag tgggatgata acgaaccccc
      781 gcggcacgga caacccggca catccggagt tgaaccccgg gccgaacaaa aggtcccggc
      841 ccggcccggg agaatccctt gcttccggca atttccgagc gcgaggagtg actcggggca
      901 tggaattgct gccatcgagc ggttcacctg atatagtcgg ctccacggtt tttgccgccg
      961 cgttccgggc aggtccctgc ctttatcctc tcctagcggc cgggaggacg gacgcggcgg
     1021 ctcttttttt ccgggccgaa atttcccgct tcacgccggt gctccgcccc ccgcaagaag
     1081 ccccttcatg agccgcgcgc tcgtcacggc gcgccccatg tagcagttgt tgaagaagac
     1141 gtaggtcttt ttcgcgcgtg ccgccatcga acggatcttc tccacccact cctccagttc
     1201 ggaacggcgg tagtcccagt cataccgatc gtgcccctca cggccccacc agctggcggc
     1261 attccgccca tggaaacgaa cgtaggccac ctcgctggtc acctcggcca ccggcgggaa
     1321 gaggcctggc aacgcgggct cgtccaccgc gatgaaaccg aggtcgagat cgccgaggaa
     1381 acggtagacc tccggccgat cccaggagac atggcgaaat tcggcccaga ggggcatcga
     1441 aggaagggcg cctcgcaacc aggccaggtg cgcgcgcgag gacgcatcgt ttcggaaagc
     1501 ccatggaaac tggagaacca cgccgtggaa gcgatcttca cggcgcatcg ggtcgaggca
     1561 ctcgaggaac tgtcgatacg tttccggatc gcggctcctc ctgtgcgtca tggcctcgtt
     1621 ggccttgacc gtgaactcga atccgggagg cgtcttttcg acgagcttca tcatcaccgc
     1681 cggatgcgga atgcgatagt aggtggagtt gatctcgacg cagggaaagt gctggacgta
     1741 gaaatcgagg cgctttccct tcggtgtgcc ttgcggatag aacgggcctt cccagtcgtc
     1801 gaaggaatag ccggaggtgc cgatcaggat cgggccaccg atcgattttg cggatgacgc
     1861 cgtcacgatc gttgcccttc gtccttgatc agcgccacga ccccaccggg aatcagcgac
     1921 gattccccga accgatcacg aagccggtcg cgaacttcgc caagccgctg ttgccgctgg
     1981 tcttcgggaa ggaggaatcc ggtggcgccc ggcccgacga gatggccgac attgatgccg
     2041 agcagccgaa ccgcccgccc cccgacgttg tccgccagca gcgccgccgc gacgcgaaag
     2101 aggatgcgat cgtcgtcggt cggtgcggcg agcgcacgct gtcgcgtgat ggtggtcccg
     2161 tcggaaaatc ggatccgcaa tgtcacgcga tgtccggacc gcccctcgcg acgcaaccgc
     2221 cgcgacacct tgtcggtgag cagaagcaga tgacgttcca ggcgcttccc ttccacgaga
     2281 tcgacgggaa aggtggtctc atgccccatc gaccggacag gaagcccttc gaagtacggg
     2341 actaccgatc ctcccccctc accggcggcc attcggacga ggacggctgc cgtcacaccg
     2401 aacacccttt cgagtcgggc agtcggacag gccgccagct cgccgatcgt ccggataccg
     2461 agctggtgaa gtaccgcctc cgattcgggg cccacgcccc agagtgcccc ggtgggacgc
     2521 ggccagaaca cctgttggaa atcggcacgg gtgagacggg tgagcccttc gggcttgtgg
     2581 agcgaggtgg ccatcttggc gatgagtttc gtctccccga ggccgatcga acaaccgaga
     2641 cgctgttccc ggcgaaccga atcctgtatg gcgcgagcca gcccctcccc cccatccgcc
     2701 tcggcggcaa ggccggtgag gtcgagaaag acctcatcga tcgatgccgg ctcgacaagc
     2761 ggagtgaagc gttcgaagag gcggaccagt tcctcggagg cggagaggta tcgcgccggc
     2821 tcgaccggaa ggaagatggc cgccgggcat cgagaccggg cttccgagag agacatcccc
     2881 gagcggatgc cgaagcgcct ggcgggatac gacgccgtgg tgaccacctg ccgcccggcc
     2941 ggcccacccc ccaccagaag cggcttcccc tcgagcttcg ggttggcgcg cacctccacc
     3001 gccgcgaaaa acgcatccat gtccacgtgg gcgatcgggc cggtcatcca tcaaaaatac
     3061 taaacaaatg gctggcttac aatttgttcc ttcgcaggct tacggggggc gtgtatcctg
     3121 tgatcatact tgggtttcca tgacctgctc cgcgccgatc gccgatcccg gtggcctttc
     3181 cggggttcgg cgatttccac tgtagggagg ttccgtggcc acgccgacgc cccaaccgac
     3241 cgttctgacc cggagcatca gtctcaagaa aaccccggcg gaggtcttcg cccgcttctc
     3301 ccagcagccg gggtgctttt tcctcgactc cgccctgggc gatcacgagc tctcgcgctt
     3361 ctcgtacatc ggcgccgaac cgttcttcac actgaaatcg cgtggcatgg tgatggacat
     3421 cgtcgagggg aagaaagtcc gccaccagaa ggggaatccg ctcgaggtgc tggcttcgat
     3481 gctccagcgc caccaggtgt cgagcggcac caccttggtg ccgttcatcg gcggcgcggt
     3541 gggctacatc gcctacgaat tcgggaagca caccaccggc ctcgacctga ccgcgcccga
     3601 tgacctaggc ttgccggagc tcgccttcaa tttctatcgg aagctgctgg cctacgatca
     3661 tcgggatcgc cgctggttcg ttgccgcggc cgacttctcg acctcgcatg gaccgtccgt
     3721 gcgcaagcgc ttgaccgccg acatcgacag gatggcggcc atggccgacg gctcggaacc
     3781 ggccgcaccc ccgccgccga aagtcgcggc ggacgccgcc gtcgacgcga gcgaggacga
     3841 tctcgccgtc gttcccgaat cgccgatggt gaccgatggt ccctatccct ggcggtcgag
     3901 cctgactcgc gagcagtatc tgggcgcggt gcgggccatc caggaacgga tcggccgggg
     3961 cgacatctac caggcgaacc tcactcagcg gtttcacgcc acgattccgt cgagcccggt
     4021 gcggctctac caggcgttgc gcgaggtgaa cccggcgccg tacggctgtt atctcgacct
     4081 tggggagtgc gtcatcgccg ggcagtcacc ggagcttttc ctgtcgatcc gggggcgcca
     4141 tgtcgaaacg cggccgatca agggaacgcg gcc
//
LOCUS       DASVOD010000530         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_921371, whole genome shotgun sequence.
ACCESSION   DASVOD010000530 DASVOD010000000
VERSION     DASVOD010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_921371"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF720_11805"
     CDS             complement(<1..547)
                     /locus_tag="VF720_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7880089.1"
                     /translation="MSGSLAERQSRAAVWFLLPAMGMIALFFVLPVLGGAALSLTDFD
                     VYAIGDPGTARFVGLRNYLDTLKHPVFQVAVRNTIYFAIVGAPLSVLVSLGAAMLLHV
                     KGLRLKPLFRTVYFTPVVTTLVAVAIVWRYLYHPRHGLVNHALGFFGIPPIDWLGDPA
                     WSMPAIILLAVWKNFGYNMLIF"
     gene            complement(544..1830)
                     /locus_tag="VF720_11810"
     CDS             complement(544..1830)
                     /locus_tag="VF720_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX7880090.1"
                     /translation="MTRGPAALAFLLLLAGCASKPDRTVTLRFWGMGREGEVVSELVP
                     EFERRHPGVRIEVQQIPWSAAHEKLLTGYVGRSMPDLFQLGNTWVSEFAALGALEPLD
                     ARVAASTVVDPESFFSGIWDTNVVDGVTWGLPWYVDTRVIFYRKDLLREAGFDSFPGD
                     WDGWRRAMDAMKARAGNDRYVLFLPLNEWNPQAILGLSAGSSLLGARDTKGAFSGPEF
                     RRGFDFYLACFNDRLAPPFAHADIANVYQEFDRGYISMWITGPWNVGEFRRRVPADRQ
                     DTWGTAPFPGPTGPSDGLSLAGGSSLAIYRGSPHREVAWQFVEYLSEVDTQLRFHRLT
                     GDLPARIEAWRDTALSNDPEMMAFGEQLRRVVSTPKVPEWEQIAFRLQERTEQAVRGA
                     ATPAVALGELDKDVDKILEKRRWLVEKRAADRAHLP"
     gene            complement(1851..>1979)
                     /locus_tag="VF720_11815"
     CDS             complement(1851..>1979)
                     /locus_tag="VF720_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7880091.1"
                     /translation="LGIDQGPILAMAENHRTELVWRTMRKNPHIVRGLKAAGVTGG"
BASE COUNT          347 a          641 c          701 g          290 t
ORIGIN      
        1 tgaagatgag catgttgtag ccgaagttct tccagacggc caggaggatg atggccggca
       61 tcgaccaggc ggggtcaccc agccagtcga tgggtgggat gccgaaaaag ccgagcgcgt
      121 ggttcacgag gccgtgccgc gggtgataca ggtagcgcca gacgatggcc accgccacga
      181 gcgtcgtgac gacgggcgtg aagtagaccg tgcggaagag gggcttcagc cggagcccct
      241 tcacgtggag cagcatcgcc gcgccgagcg acacgaggac cgacagcggc gccccgacga
      301 tcgcgaaata gatcgtgttg cgaacggcca cctggaagac ggggtgtttg agcgtgtcga
      361 ggtaattcct gagccccacg aaccgggcgg tcccggggtc gccgatggcg tacacgtcga
      421 aatcggtcag tgagagcgcc gcgcctccca gcaccgggag cacgaagaag agggcgatca
      481 tccccatggc cggcaggagg aaccagacgg ccgcgcggga ctgccgctcg gcgagcgatc
      541 cgctcatgga agatgggccc ggtcggcggc gcgtttctcg acgagccacc gacgcttctc
      601 gagaatcttg tccacgtcct tgtcgagctc gcccagcgcc acggcgggcg ttgccgcgcc
      661 gcgcaccgcc tgctccgtgc gctcctgcag tcggaaggcg atctgctccc attccggaac
      721 cttgggcgtc gacacgacgc gccggagctg ttccccgaag gccatcatct cggggtcgtt
      781 cgagagggcc gtgtcacgcc aggcctcgat gcgggcgggg aggtcgccgg tgaggcggtg
      841 gaagcggagc tgcgtgtcga cttccgacag gtattccacg aactgccagg cgacttcgcg
      901 atggggcgaa cccctgtaga tggccaggct cgatccgccg gccagcgaga ggccgtccga
      961 cggccccgtc ggtccgggaa acggcgccgt gccccacgtg tcctgccggt cggcaggcac
     1021 gcgccggcga aactcgccca cgttccaggg ccccgtgatc cacatgctga tgtagccgcg
     1081 atcgaactcc tggtagacat tggcgatgtc ggcgtgcgcg aagggtggcg cgaggcggtc
     1141 gttgaagcag gccaggtaga agtcgaagcc gcgccggaat tcaggaccgc tgaaggctcc
     1201 cttcgtgtcg cgcgcgccga ggagtgagga acccgcggaa agacccagga tggcctgcgg
     1261 attccattcg ttcagcggca ggaagagcac gtaccggtcg ttccccgccc gggccttcat
     1321 ggcgtccatg gcgcggcgcc agccgtccca gtcgcccggg aacgaatcga agcccgcttc
     1381 gcggaggaga tccttccgat agaagatgac gcgcgtgtcg acgtaccagg ggagccccca
     1441 cgtgacgccg tccaccacgt tggtatccca gatccccgag aagaatgatt cgggatcgac
     1501 gaccgtcgag gcggccacgc gggcgtccag cggctcgagg gcaccgaggg cggcgaactc
     1561 ggatacccag gtgttgccga gctggaagag gtcgggcatg gagcgtccca cgtatccggt
     1621 caggagtttt tcgtgggcgg ccgaccacgg gatctgctgc acctcgatgc gcacgccggg
     1681 gtgccgccgc tcgaactccg gcacgagctc ggagacgact tcgccttccc gccccattcc
     1741 ccagaagcgg agcgtgacgg tccggtcggg cttcgaggcg caccccgcga gcaggaggag
     1801 gaacgccagc gcggcgggcc ctctcgtcac cgcacgacca cagaatcgag ctagccgccc
     1861 gtgacgccgg ccgccttgag gccgcgcacg atgtgcggat tcttcctcat cgtgcgccag
     1921 acgagttcgg tgcgatggtt ctcggccatg gcgaggatcg gcccctggtc gatgccgag
//
LOCUS       DASVOD010000531         7867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_869432, whole genome shotgun sequence.
ACCESSION   DASVOD010000531 DASVOD010000000
VERSION     DASVOD010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869432"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..750
                     /locus_tag="VF720_11820"
     CDS             22..750
                     /locus_tag="VF720_11820"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX7880092.1"
                     /translation="MSSIAPASRETPPTRSVYSDECLETLARWRGIDAKPDPENRRKK
                     NGIRIFRYKLMELGLTKSPWYVPWLFTIPIMGGGLWWGYSHGLPAWQLVVGFFAGILL
                     WTLLEYLLHRWLFHYTPKSETLKVFFFLTHGYHHEFPDDPMRLVAPPLMYWSLASFFA
                     LGFFLAFGKVWWGPLYAGMMGGYLFYDSVHYYVHHARPTSGPGKFLRSYHLLHHFDDH
                     DTRYGISTPLWDFVFRTYRPVGKK"
     gene            975..1700
                     /locus_tag="VF720_11825"
     CDS             975..1700
                     /locus_tag="VF720_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880093.1"
                     /translation="MRSVINVVAALSLVTLGAVDSRADLTGSTTYDVFVGGSRVGSER
                     LTWTEADGGQVTLEGECVLEVGGISTTLRPSLTIDAATLSPTRYAREMELGDRVDELE
                     AVFEGGKAVMSVKENGSSSKRSIKMKPAELVVDGHVMSLLTLVADRYDFDKGGEQDLT
                     VFDTEEGKAHAAHAMVRGMGTFENTSGSWRMKRLTVKLENVSVDLLVDDAGRVPLISM
                     PLRNMEARLANYAGNSKAEAGAK"
     gene            complement(1958..4504)
                     /locus_tag="VF720_11830"
     CDS             complement(1958..4504)
                     /locus_tag="VF720_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7880094.1"
                     /translation="MASGTLLAIALATPGLLPASVGHGTPGDLPATLDGRFRAAFRSP
                     FAARRALAPAPDGTVGCFVEGPVSPQALEHLGARLGATAGTRRTMRIPAARLLEAARL
                     PGVRRISPALPMTPQLDLSGNDTRATLVHQGSPDTPNGVQGNGVLVGIIDSGIDWSHA
                     DFKKLGGTTRIRAIWDQQDPIGPPPAGYPYGTAWTQAQIDMGMCRERDSTYYGHGTHV
                     AGIAVGSGRGTGNGWPQFTYVGVAPRADIIAVNTTFDDVDIADGIAWVFAQATTLGKP
                     AVVNLSLGSDYGPHDGTGDLDQFINAVSGPGRMVVASAGNSNGKGRHGERIVPTVGDG
                     VLSFRIFGYTPSPSNPDGISVDLWYEGGDNHMVTVRTPGGFSVGPVAKGGMLTQATPD
                     GWVSIDATQVEGTHDNGDVEVLVEVSDAAGVPPASGDFEVRFTRVSGSLASEVDGWIY
                     DFSFPFPPAFVSGKEEAELVISPSTADSAICVAAHTTRASWQAINGNVYNYGQTLNQI
                     GTFSSPGPRRDAVAKPDLSAPGTAIGAAHSVTSLPNPTSNVLPDGVHKILQGTSMSAP
                     QVAGAAALLLSRWPMLSATQVRTALMQSARADGFTGGVPNNTWGAGKLDLDQLFRQRL
                     AFTTTVDPARAATPGSSVGLLELCAMPAGGTWSTRYRVRVSDSQGWLKLSNGGPPVAV
                     ALYDTITSAAIPGQARCAPLAGTLLIAIPPGTPNGTSTTVTFQVEPEGVPWMGVTRVT
                     TVTVSTTSDVAALDLPKRLTLASRPRPVDGSFELEMALPHAGHVRLDAYDVTGRLRAT
                     LVDRSLEAGWTRIPWTGVDDAGRRLAAGVYLLRLVSPDGTASGRVVLTPG"
     gene            complement(4605..6620)
                     /locus_tag="VF720_11835"
     CDS             complement(4605..6620)
                     /locus_tag="VF720_11835"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7880095.1"
                     /translation="MIRRILFALLLTGALTTSASAWLPDNPLADPTFEEILQLENDRA
                     TSGRIVELTKDNNPLVRARAFRALARAQDPATLALFTAGLADTDPAVRDEAAFGMGLL
                     WADGREQDLVEAYARETDPRVKATLIEALGRTTDGGAGMTLLASTVTSADTTLSYRAC
                     LAVGVAGYRKVDITATVDSLGRAARSPHAGTRWASAYALFRGKPEASVQILRPLLADR
                     DPLVRMNAVRALSASKRNNLALPISELVRDADWRVRLEAIKGLATLKATQFANLMSLG
                     LDDEVPVVRSGTFQALGELRSQVVLDRITPLVTESDDWRIRTQALYTKTQIEVDGTLP
                     LLEQLKTNNDWHIRRSAAQSMGLLASDQARAILGTMVSDSDPRVLAAVAEALTNYPQV
                     AALEDLRHLLTSEDLAVLTTSASALGARGDRGALGGLNAAYARLKSPADSEPMTEIVK
                     ALGNIVVPLDSTVVYGSLDKSDRDATIATLTAAMKDPDRNVALAAAASLGRIDGKDHA
                     GEVAPASSGSYPLNLDVIRKTEASRARIVTGKGEIVIEFLPGAAPNTVANFVTLVSRG
                     YFNGLTFHRVVPGFVTQDGCPRGDGWGGPGYAIRCEYNDLHYEAGMVGMALSGKDTGG
                     SQYFITQTPQPHLDGKYTIFGRVISGLSVLEEILVGEPITRVDLIPS"
     gene            6732..>7867
                     /locus_tag="VF720_11840"
     CDS             6732..>7867
                     /locus_tag="VF720_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002621818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HEX7880096.1"
                     /translation="MNDQPPLRIPEILAPAGDDECLTAALNAGADAVYFGLAEGFNAR
                     ARATNFTLDSLPGTVERIHRHGARAYVTMNTLVFDSELGAVEAILRRLAGAGIDAILV
                     QDPAVARLATEVGVPFERHASTQMTLSSPEGMSLARDLGLSRVVLPRELTLEEIALLR
                     RSTDLELEVFVHGALCMSWSGQCLTSEAWGGRSANRGQCAQSCRLPYDLVVDGVTRDL
                     GSVAHLLSPHDLAAWRVIPRLIELGIASLKIEGRQKGPAYVTTAVEAYRAARDRVVAR
                     ARPDEELLPREAIDAMAVSFSRGFSTGFFEGVNHQQLVDGTSPTHRGLRVGIVDRVEG
                     HEVILQPESDRPGLKPGDGVVFAADREGGTETGGPLFAVAVRADG"
BASE COUNT         1263 a         2520 c         2760 g         1324 t
ORIGIN      
        1 ccaaggaggt ttggttcgtt catgtccagc attgcccccg cctcgcgaga gaccccgccg
       61 acacgctccg tctactccga tgaatgcctc gaaacgctcg cccgctggcg cgggatcgac
      121 gcgaaaccgg atcccgagaa ccgtcgcaag aagaacggca tccggatctt ccgctacaag
      181 ctgatggaac tgggcctcac caagtcgccc tggtacgtgc cgtggctctt caccatcccg
      241 atcatgggcg gggggctctg gtgggggtat tcgcacggac tgccggcctg gcagctggtg
      301 gttggtttct tcgcgggcat cctcctctgg acgctcctcg aatacctgct tcatcggtgg
      361 cttttccatt acacgccgaa gtcggagacg ttgaaggtct tcttcttcct cacgcacggc
      421 tatcatcatg aattccccga tgacccgatg cggctggtgg cgcctccgct catgtactgg
      481 agcctcgcct cgttcttcgc gctgggcttc ttcctggcct tcgggaaggt gtggtggggg
      541 ccgctctacg ccggcatgat ggggggatac ctcttctacg acagcgtgca ctactacgtg
      601 caccatgccc ggccgacgtc gggccccggg aagttcctcc ggagttacca cctcctgcat
      661 cacttcgacg atcacgacac ccgctacggc atctcgacgc ccctgtggga cttcgtcttc
      721 cgcacctacc gccccgtcgg taagaaatag cctcaccgtc ccccggttga tgggtacggg
      781 cgacccacca gtgcccccgt tcgtttccgg acgccaaccg ttcgggcctc cagtcgaata
      841 tttacaaagg gccatgcaac tttccccgtg tggcccgcgt ctactcgccc ggttcccgta
      901 atcgtcggca cgcgcatcac ccgcagggct tcgggtcgag cacacgccga cggcggcgaa
      961 tggagggctt gaacgtgcgt tccgtaatca atgtcgtggc cgcgctgtcg ctggtgactc
     1021 tgggcgcggt ggactcccgg gccgatctca ccgggagcac gacgtacgat gtctttgtgg
     1081 gtggcagtcg ggtcgggtcg gagcgattga cgtggacgga ggcggacggc ggccaggtca
     1141 ccctcgaggg ggagtgtgtc ctggaagtcg gtgggatctc caccacgctc cggcccagcc
     1201 tgacgatcga cgccgcaacg ctgtcgccga cgcgctacgc gcgggagatg gaactgggtg
     1261 atcgcgtgga cgagctcgaa gcggtcttcg agggcggcaa ggccgtgatg tcggtcaagg
     1321 agaatggcag cagctcgaag cgaagcatca agatgaagcc ggccgaactc gtggtggatg
     1381 gtcacgtgat gtcgctgctc acgctcgtcg ccgatcgcta cgatttcgac aagggtggcg
     1441 agcaggacct gacagtgttc gataccgaag agggaaaggc gcatgcggcc cacgccatgg
     1501 ttcgcggcat gggcaccttc gagaacacga gcggctcgtg gcggatgaag cggctcacgg
     1561 tgaagctgga aaacgtctcg gtcgacttgc tcgtcgatga cgccggtcgg gtgccgctga
     1621 tctcgatgcc gctcaggaac atggaagcac gcctggccaa ttatgccggg aacagcaagg
     1681 cggaggccgg ggcaaagtaa tcagggacca gcccgggcat cccccggttg tcagccggaa
     1741 agtggctggt aaggggtacg ggcggcggac cttcgcggac gagggcggtc ggcgtcagtc
     1801 gacaacgcat gactcgtctg cccgggtcgg agtaccggtc ggcggggacc accggatgct
     1861 gcgatcggga ccgcgaagca agccgtccgt accccgtttt cctgcgcaag atcaggttta
     1921 tcggttcggc tgcgatcgtt cagccgggtc gagccgatca gcccggggtg agaaccactc
     1981 ggcccgaggc cgtaccgtcc ggcgagacca gccgcagcag gtagaccccg gcggcgaggc
     2041 ggcggccggc gtcatccact cccgtccagg ggattcgtgt ccaccccgct tcgagcgacc
     2101 gatcgacgag agtggcccgc aggcgcccgg ttacgtcgta ggcgtcgagc cggacgtgcc
     2161 cggcatgagg gagcgccatc tcgagttcaa agcttccgtc gacgggccgg gggcggctgg
     2221 ccagcgtgag tcgcttcgga aggtcgagag ccgccacatc cgaggtcgtg cttaccgtca
     2281 cggtcgtgac tcgcgtcacg cccatccacg gaactccctc cggttccacc tggaacgtga
     2341 cggtggtcga cgttccgttc ggggtgccgg gtgggatggc gatcagcagt gtgccggcca
     2401 gcggggcgca tcgcgcctgc ccggggatgg cggccgatgt gatcgtgtcg tagagcgcca
     2461 ccgccaccgg gggtccgccg ttcgacagct tgagccaccc ctgcgagtcc gacacgcgaa
     2521 cccgatagcg ggtgctccac gtgccgccgg cgggcatggc acagagctcg agcaggccca
     2581 cgctgcttcc cggagtcgct gcgcgggcag gatcgacggt ggtggtgaac gccagccgct
     2641 ggcggaagag ctggtccagg tcgagcttgc cggcgcccca ggtgttgttg ggcacgccgc
     2701 cggtgaagcc atccgcgcga gccgactgca tgagggcggt ccgcacctgc gtggccgaga
     2761 gcatgggcca gcgcgacagg aggagcgccg ccgcacccgc cacctgcggt gcggacatgc
     2821 tcgttccctg gaggatcttg tggaccccgt cggggagcac gttggaggtg gggttcggaa
     2881 ggctcgtcac cgagtgcgcg gcgccgatcg cggtgcccgg ggcggaaagg tccggcttgg
     2941 ccacggcgtc gcggcgtggt ccggggctcg aaaacgtgcc gatctggttc aaggtctgac
     3001 cgtagttgta gacgtttccg ttgatcgcct gccacgaggc ccgggtggta tgggcggcaa
     3061 cgcaaatggc gctgtcggcc gtcgaggggc tgatgacgag ctcggcctcc tccttgccac
     3121 tgacgaaggc cggcgggaac gggaatgaaa agtcgtagat ccagccatcc acctcgctgg
     3181 ccagggatcc cgagacgcgc gtgaaacgga cttcgaagtc gccgctggcg ggagggacac
     3241 cggcggcatc ggagacttcg accagcactt ccacgtcgcc gttgtcgtgg gtgccctcca
     3301 cctgcgtggc gtcgatcgac acccagccgt cgggagtggc ctgtgtgagc atccccccct
     3361 tggcaaccgg gccgaccgag aacccgccgg gcgtgcggac ggtcaccatg tggttgtcgc
     3421 ctccctcgta ccagaggtcg acggagatgc cgtccgggtt ggacgggctc ggcgtgtagc
     3481 cgaagatgcg aaaggagaga acgccgtcgc cgaccgtcgg cacgatgcgc tcaccgtgcc
     3541 gtcccttgcc gttggagttg ccggcgctgg ccaccaccat ccggcccggt cccgagacgg
     3601 cattgatgaa ctggtcgagg tctcccgttc catcatgcgg gccgtagtcg ctgccgaggc
     3661 tcaggttcac caccgccggt ttgccgaggg tcgtggcctg ggcgaagacc cacgcgatcc
     3721 cgtcggcgat gtccacgtca tcgaacgtgg tgttcacggc gatgatgtcg gcgcgcgggg
     3781 ccacgccgac gtaagtgaat tgcggccacc cgttcccggt cccgcggcca ctgccgaccg
     3841 cgatgccggc cacgtgggtg ccgtgcccgt agtaggtgct gtcccgttca cggcacatac
     3901 ccatgtcgat ctgggcctgg gtccatgcgg ttccgtacgg atagccggcg gggggcggcc
     3961 cgatcggatc ctgttgatcc cagatggccc ggatgcgggt cgtgccgccc agcttcttga
     4021 agtcggcatg ggaccagtcg atgccggaat cgatgatccc caccaggacg ccgttcccct
     4081 ggacgccgtt cggcgtgtca ggggatccct ggtgaaccag cgtggcccgc gtgtcgtttc
     4141 ccgacaggtc gagttgcggc gtcatcggca gcgccggcga gatgcgacgc accccgggaa
     4201 gacgggccgc ctcgaggagc cgcgctgccg gaatgcgcat cgtgcgccgg gtgcctgccg
     4261 tggcgccgag gcgggcgccg agatgctcga gcgcctgggg cgagacgggt ccttccacga
     4321 aacagcccac cgtgccgtcg ggggccgggg caagcgcccg tcgggccgcg aaaggcgatc
     4381 ggaacgccgc ccggaatctc ccgtcgagcg tggccggcag gtcacccggg gtgccatggc
     4441 cgacactcgc aggaaggagg ccgggagttg ccagggcgat ggcgagaaga gttcccgacg
     4501 ccacgcgtcg cgaagcatgg gacattgccg gtagtgcagg gagcggcttc cctcgaaagg
     4561 tcatgcgaat cacggtgtct ccgtcgccgc cccgggggtg gcaactatga gggaatcagg
     4621 tcgaccctgg tgatcggttc acccaccagg atttcctcca gcacggaaag gccggagatc
     4681 acccggccga agatcgtgta cttgccgtcg aggtggggct ggggtgtctg cgtgatgaaa
     4741 tactggcttc cgccggtgtc cttgccggag agcgccatgc cgaccatccc ggcttcatag
     4801 tggagatcat tgtactcgca ccggatggca taaccgggcc cgccccatcc gtccccgcgc
     4861 ggacagccgt cctgggtgac gaaaccgggg acgacccggt ggaaggtaag gccgttgaaa
     4921 tagccgcgcg aaaccagcgt cacgaagttg gcgacggtgt tgggcgcggc ccccggcagg
     4981 aactcgatca cgatttcccc cttgccggta acgatccggg cgcgcgaggc ttccgtcttg
     5041 cggatgacgt cgaggttgag gggatagctg ccgctggagg cgggggccac ttctcccgcg
     5101 tgatccttcc cgtcgatgcg gccgagtgag gcagcggccg ccaaggccac gttccggtcc
     5161 ggatccttca tggctgcggt gagcgtggca atcgtcgcgt cccgatccga cttgtcgagc
     5221 gatccataca cgacggtgga atcgagcggc accacgatat tgcccagcgc cttcacgatc
     5281 tcggtcatcg gctcactgtc cgccggcgac ttcaggcggg cataagcggc gttcagcccg
     5341 cccagcgcgc cccggtcacc ccgggctcca agggccgagg cactggtggt gagaacggcc
     5401 aggtcttcgc tcgtgagcag atgacgcagg tcctccagcg ccgccacctg cggatagttc
     5461 gtgagcgcct cggccaccgc ggccagcacg cggggatcgg agtcgctcac catcgtaccc
     5521 aggatggcac gcgcctggtc cgaggcaagg agtcccatgg actgtgcggc cgaccgccga
     5581 atgtgccagt cgttgttcgt tttcagctgt tcgagcaggg ggagggtgcc gtcgacctcg
     5641 atctgcgtct tcgtgtacag cgcctgggtc cggatccgcc agtcgtcgct ctccgtgacg
     5701 aggggtgtga tgcgatccag taccacctgc gaccgcaatt cgccgagggc ctggaacgtg
     5761 ccgctcctga cgacgggcac ttcgtcgtcg aggcccaggc tcatgaggtt cgcgaactgg
     5821 gtggccttga gcgtggcaag ccccttgatg gcctcgagac gaacgcgcca gtcggcatcg
     5881 cgaaccagtt cggaaatcgg gagcgccagg ttgttgcgct tgctggccga cagggcgcgg
     5941 accgcgttca tgcggacaag cggatcacga tcggcgagga ggggcctcag gatctggacg
     6001 gatgcctccg gtttgccgcg aaagagcgcg taggcagagg cccaccgcgt gccggcatgc
     6061 ggcgaacggg ccgctcttcc cagcgaatcg acggtggcag tgatgtcgac cttgcggtaa
     6121 ccggccacac cgaccgccag gcaggcgcgg taggagaggg tcgtgtcggc cgacgtgacc
     6181 gtcgaggcca ggagggtcat gccggctccg ccgtccgtcg tgcgtcccag cgcctcgatc
     6241 agcgtggcct tgacccgggg gtccgtctcc cgggcataag cttcaaccag gtcctgctcc
     6301 cgtccgtcgg cccagaggag ccccatgccg aaagcggcct catcgcggac ggcagggtcg
     6361 gtatcggcaa gccccgcggt aaagagcgcc agagtggccg gatcctgggc ccgggccagc
     6421 gcccggaagg cccgggcgcg taccagggga ttgttgtcct tcgtcagctc gacgatgcga
     6481 ccgctggtgg cacggtcgtt ttcgagctgc aggatttctt cgaaggtggg gtccgccaag
     6541 ggattatcgg gaagccaggc cgaggcggag gtcgtcaggg cgccggtcag gagcagcgcg
     6601 aacagtatcc gacggatcaa ggatcctcct tctactaagt gtgtgattta caatacgttc
     6661 aagtatacag agggtccgcc gggaggcgca atttcttccc gtcggagagg ctgcagggac
     6721 cgggacccga catgaacgat caaccaccac ttcgaattcc agaaatcctc gctcccgcgg
     6781 gggatgatga gtgcctgacg gcggccctga acgccggggc cgatgccgtc tacttcggcc
     6841 tggccgaggg gttcaacgcg cgggcccggg ccacgaattt cacgctcgac tcgcttcccg
     6901 gcaccgtgga gcggatccac cggcatggcg cccgggccta cgtgacgatg aacacgctgg
     6961 tcttcgacag cgaactgggg gcggtggaag ccatcctccg gcggctggcc ggcgccggga
     7021 tcgacgcgat cctggtccag gacccggcgg tggcccggct cgcgacggag gtcggtgtcc
     7081 cgttcgagcg gcatgcctcc acccagatga cgctgtcgag ccccgaggga atgtcgctgg
     7141 cgcgcgacct cggcctctcg cgcgtggtgc tccctcgtga gctcaccctc gaggagatcg
     7201 cgctgctgcg tcgatccacc gatctcgagc tcgaggtttt cgtgcacggg gcgctctgca
     7261 tgtcgtggag tggtcagtgc ctgaccagcg aagcgtgggg tgggcgatcc gccaaccggg
     7321 ggcagtgcgc acagtcgtgc cgccttccat acgacctggt cgtggacggc gtgacgcgcg
     7381 acctggggag cgtggcgcac ctcctttccc cgcacgacct cgcggcgtgg cgggtgattc
     7441 cgaggctcat cgaactggga atcgcctcgc tcaagatcga gggaaggcag aaggggccgg
     7501 cgtacgtgac gacggcggtc gaggcctatc gtgcggcgcg ggaccgggtc gtggcccgcg
     7561 cacgcccgga cgaggaactc ctgccgcggg aagccatcga tgccatggcg gtcagtttct
     7621 cgcgcgggtt ctcgacgggg ttcttcgaag gggtgaatca ccagcaactg gtcgacggca
     7681 ccagcccgac tcaccggggc ttgcgcgtcg gaatcgttga tcgggtggag ggtcatgagg
     7741 tgatcctgca accggagtcc gaccggcccg ggctcaagcc cggcgatggc gtcgtcttcg
     7801 ccgccgatcg tgaagggggc accgagaccg gtggcccact cttcgccgtg gcggtccggg
     7861 cggatgg
//
LOCUS       DASVOD010000532         3927 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_37426, whole genome shotgun sequence.
ACCESSION   DASVOD010000532 DASVOD010000000
VERSION     DASVOD010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37426"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1416)
                     /locus_tag="VF720_11845"
     CDS             complement(97..1416)
                     /locus_tag="VF720_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7880097.1"
                     /translation="MRRIVITVKSFLLLAAVVIGKVPSAEAEAVVLKARWLFDGTNAS
                     LQSPGLVVVDGNRIVAVGPGAEIPAGARTIDLGDATLCPGFIDAHTHLCHERTDDWKG
                     DELDQFKKLIPQQSLEASQLTKETLHAGFTTCRDLGANDMVDVALRNAINAGVIEGPR
                     MLVAVHAIGATGGHCDPTAGYRPDIYGNAMEEDGVADGPDAIRRAVRLNHKFGADVIK
                     VCATGGVLSLTDDVDSPQLTQAELDALVEESHALRRKTAAHAHGAEGAKRAIRAGIDS
                     IEHGTFLDDEALKLMKQKGTVLIPTLMAHQGLKEMMDGNVKMPAAVRAKAEAASAALS
                     TTMKRAVELGVRIGLGTDAGVYPHGRNPEEFAQLVSHGLSPIAALMAGTSVDAELLGW
                     SDRIGALEAGKLADIVAVPGDPTADIRATEKVFFVMKDGVIYRNDAR"
     gene            1546..2265
                     /locus_tag="VF720_11850"
     CDS             1546..2265
                     /locus_tag="VF720_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7880098.1"
                     /translation="MSDDERRFEELQNALAIRHEMLEVVAHDLRGPLNILTMGLEILR
                     HSAPPGQESNIERMRRAIDSMSDLVTNLIDLSHIREGRQVLNVATIYPDVLLRDVFLM
                     QQPLVARRGLGLEHEAARNLPPVSADYERLLRVFADLVNHSARKALPGSTIRIEADAW
                     TNGDVPAVSFLVTHEGTPIRQDELGLDVDTYFRLGTPERKIADLGLAIARAVIEAHGG
                     RVVPCRHPTGEPSIRFWLPGA"
     gene            complement(2296..>3927)
                     /locus_tag="VF720_11855"
     CDS             complement(2296..>3927)
                     /locus_tag="VF720_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX7880099.1"
                     /translation="ILYLCGVLTVKGGTNRVIEYFGPGTATISATGKGTICNMGAELG
                     ATTSIFPWDDRMATYFRSTGREALVRIAEANRALLTPDPEVESDPKRYYDQIVEIDLS
                     KLEPHLVGPHTPDLARPVSAVAGAVKEHDYPDRIAVSLIGSCTNSSYEDIERATDVAR
                     QARAHGARMKTPLLVTPGSEQVRATIERDGQLGELEAVGATVLANACGPCIGQWKRDE
                     IRKGEKNTIVSSFNRNFPARNDGNRETLSFIGSPEIVMAYGLAGRLSFNPLTDELEGP
                     NGRYRLTPPKPAPEVPPGGFIPDTAGYLAPAADAGKVQVAVSPTSERLQLLEPFPAWN
                     GKDFEKLPILLKARGKCTTDHISPAGPWLRFRGHLDRISDNMFLGAVNAYTGDAGTTK
                     NLLSGETGNSVPAVARDYKSRGLQWVVVGDENYGEGSSREHAAMSPRFLGAAAIIVRS
                     FARIHESNLKKQGVLPLTFADPADYDKVREDDRLSLTGLGALAPGKPVSAILHHADGA
                     TDTLTLHHTLNAEQIDWFKAGSALNLLRQKAARIG"
BASE COUNT          591 a         1305 c         1336 g          695 t
ORIGIN      
        1 acagtggccg cccagccttc ggccgccggg agtcccgcgg tggagctggc cggcatcgcc
       61 cgaggcctga ccgcccgcca gccggcctga ccactgctac cgggcgtcgt tccggtagat
      121 cacgccgtcc ttcatcacga agaacacctt ttccgtggcc cggatatccg ccgtgggatc
      181 ccccggcacg gccacgatgt cggccagctt cccggcctcg agggccccga tccggtcgga
      241 ccagccgagg agttccgcgt ccaccgaggt gccagccatc agcgccgcga tgggcgacag
      301 gccatgactg acgagctgcg cgaattcctc gggattgcgg ccatgcggat aaacgccggc
      361 atcggtgccc aggccgatcc tgacccccag ctcgacggcc cgcttcatgg tggtgctgag
      421 cgcggcgctg gcggcctctg ccttggcccg gaccgcggcg ggcatcttca cgttgccgtc
      481 catcatctcc ttgagaccct gatgtgccat cagggtggga atcagcaccg tccccttctg
      541 cttcatcagt ttcagcgcct cgtcatcgag gaaggtgcca tgctcgatgg aatcgatgcc
      601 ggcccggatg gcgcgcttgg ctccctcggc gccgtgtgcg tgggcggcgg tcttccgacg
      661 gagtgcgtgc gattcctcca ccagggcatc gagctccgcc tgggtgagct gcggtgagtc
      721 gacgtcgtcg gtgagactga gcacgcctcc ggtggcgcac accttgatga cgtcggcgcc
      781 gaacttgtgg ttgaggcgga cggcgcggcg gatggcgtcc gggccgtccg ccaccccatc
      841 ctcttccatg gcattgccgt agatgtcggg acgatagccg gccgtcggat cgcagtgacc
      901 gccggtggcc ccgatggcat gcaccgccac cagcatccgg ggcccttcga tcacgccggc
      961 gttgatggcg ttccggaggg ccacgtcgac catgtcattg gcgccgagat cccggcaggt
     1021 ggtgaatccc gcgtggaggg tttctttcgt gagctgcgac gcctcgagtg actgttgcgg
     1081 gatcagcttc ttgaactggt cgagctcgtc ccctttccaa tcgtcggttc gctcgtggca
     1141 caggtgtgtg tgggcgtcga tgaagcccgg gcagagggtg gcatccccca gatcgatcgt
     1201 gcgggcgccg gcaggaattt cggccccggg acccacggcc acgatacggt tcccgtccac
     1261 caccacgagt ccggggctct gcaggctggc gttcgtgccg tcgaagagcc agcgggcctt
     1321 gagcaccacg gcttcggcct ctgcgctggg gactttcccg atgacaactg cggcgaggag
     1381 aaggaagctt ttgacggtga tcacgatgcg gcgcattaag gtatctccgg gaggaacagc
     1441 cggggagcgg taccggaatc gcgcggcact ataccccact ccggctttcc cgtcccatgc
     1501 ctgccatggc gacactcctc cgttgccggg atcgaagatg acccgatgag cgacgacgag
     1561 cgccgcttcg aggagctcca gaacgcgctg gccatccgcc acgagatgct cgaggtggtg
     1621 gcccacgacc tgcgcgggcc cctcaacatc ctcaccatgg ggctcgagat cctgcgccac
     1681 agcgcgccgc ccggacagga atcgaacatc gagcggatgc gccgggcaat cgactcgatg
     1741 agcgacctgg taacgaacct gatcgacctc tcgcatatcc gcgaagggcg acaggtgctg
     1801 aacgtggcca cgatctaccc cgacgtgctc ctgcgggacg tcttcctgat gcagcagccc
     1861 ctggtggcac ggcgcgggct gggactcgag cacgaggccg cccgcaacct ccctcccgtg
     1921 tcggccgact acgagcggct gctgcgcgtc ttcgccgacc tggtgaacca ctccgcgcga
     1981 aaggcgctcc ccgggtccac gatccggatc gaagccgatg cctggacgaa cggcgacgtg
     2041 cctgccgtga gcttcctggt gacgcacgaa ggaacgccga tccgccagga cgagctcggc
     2101 ctcgatgtgg acacctactt ccggctcgga actcccgagc ggaagatcgc cgacctgggc
     2161 ctcgcgatcg cacgggcggt catcgaggcc catggtggcc gggtggtgcc ctgccggcac
     2221 ccgaccggcg aaccttcgat acgtttctgg ctgccgggcg cctgacgagc atccggcacc
     2281 gaccccttcc ggccgctacc cgatcctggc ggctttctgc cggagcagat tcagcgcact
     2341 ccccgccttg aaccagtcga tctgttcggc attcagggtg tggtgcaggg tgagcgtgtc
     2401 agtggcgcca tcggcgtggt gcaggatggc cgacacgggc ttccccgggg ccagcgctcc
     2461 gaggccggtg aggctcagcc ggtcgtcttc acgcactttg tcgtaatcgg ccgggtcggc
     2521 gaaggtgagc ggcagcactc cctgcttctt gagattcgat tcatgaatcc gggcgaacga
     2581 ccggacgatg atggccgcgg ccccgaggaa ccggggcgac atggcggcat gctcgcggct
     2641 cgagccttcg ccgtagtttt cgtcgccgac gaccacccac tggaggccgc gcgacttgta
     2701 atcacgtgca accgccggaa cggaattgcc ggtctcgccg ctcaggaggt tcttcgtggt
     2761 gccggcatcg ccggtgtacg cgttgaccgc gccgaggaac atgttgtcgc tgatccggtc
     2821 gagatggccg cggaagcgca gccacggccc cgcgggcgag atatggtcgg tggtgcactt
     2881 ccccctggcc ttgaggagga tgggaagttt ctcgaaatcc ttgccgttcc aggccgggaa
     2941 cggctcgagg agctggagcc gttcgctggt cggcgacacg gccacctgca cctttccggc
     3001 gtcggcggcc ggggcgaggt accccgccgt atcgggaatg aatccgccgg gcggcacttc
     3061 gggagccggc ttcggcggcg tgagcctgta gcggccgttc ggtccctcga gctcgtcggt
     3121 gagggggttg aacgagaggc gtccggccag tccgtaggcc atcacgatct ccgggctgcc
     3181 gatgaaggag agggtttcgc ggttcccgtc gttccgggcc gggaaattcc ggttgaacga
     3241 cgacacgatc gtgttcttct cccccttcct gatctcgtcg cgcttccact ggccgatgca
     3301 ggggccgcag gcgttggcca ggaccgtggc accgaccgct tccagctcgc cgagctggcc
     3361 gtctcgttcg atcgtggccc gcacctgttc ggagccgggt gtcacgagga gaggcgtctt
     3421 catgcgggcg ccgtgcgccc tggcttgccg ggccacgtcg gtggcgcgtt cgatgtcttc
     3481 gtaggacgag ttcgtgcagg agccgatgag cgataccgcg atgcgatccg ggtagtcgtg
     3541 ctccttcacc gcgccggcca cggccgacac ggggcgcgcg aggtcgggcg tgtgaggacc
     3601 caccaggtgc ggctcgagtt tcgaaaggtc gatctcgacg atctggtcgt aataccgttt
     3661 cggatcggat tccacttcag ggtccggggt gaggagggcg cggttggcct cggcaatccg
     3721 caccagcgcc tcgcgaccgg tgctcctgaa gtacgtggcc atccggtcat cccacgggaa
     3781 gatcgaggtc gtggcgccga gctccgcgcc catgttgcag atcgtgcctt tccctgtcgc
     3841 ggaaatggtg gcggtgccgg gcccgaagta ctcgatcacc cggttggtgc cccccttcac
     3901 ggtgaggacg ccgcacaggt agaggat
//
LOCUS       DASVOD010000533         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_88331, whole genome shotgun sequence.
ACCESSION   DASVOD010000533 DASVOD010000000
VERSION     DASVOD010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88331"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VF720_11860"
     CDS             <1..782
                     /locus_tag="VF720_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880100.1"
                     /translation="RHFPSVAFGAVVAALLSGCAPSEQEVDYEARKALLERQNQGLRE
                     LIAEAEAGTLVPADRFLVGVDESILKQVLDSQLPFEHQIGDKLGIRLERATVLLRDKY
                     GHMTLEGEVHRLATPDRKTKVRIYGGLGAVSVDSTSNRLSVKIAVDRIVLVEAGLLDA
                     VLGANGKKLLSEKGREILEDKLPVLQVPVALAQTIRVPAMADGPLQLDSLSVPLNLSV
                     DRVLAAGGKLWLTIHAELGAVQGGEEGLGVSVIKKKKPKAS"
     gene            779..>1578
                     /locus_tag="VF720_11865"
     CDS             779..>1578
                     /locus_tag="VF720_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880101.1"
                     /translation="MMRTPRFRLVPLLCVLALAAPGCARRASEAETRQWNGTLARLQA
                     EQDSLRARAAELIMADPNLARLPSDPVVIRVPTSFVREVVEHLMTSVVDNITLRLSGI
                     KAHVAKKVKKIVTIGEFKLDVEVHEVVGKLTTEKPEVGFGDGEISMSLPVNATEGTGT
                     ATLHFQWDGKRLADLTCGDLDVTKTLTGQVVPARYIIAGRMGLAVTDNKIVCTPIFPE
                     TRIRLRVEPTKAAWDTVNALLAEKHGVCGWVLDKVNVPSILEGIIQEKG"
BASE COUNT          269 a          541 c          533 g          235 t
ORIGIN      
        1 tccgacattt cccatcggtc gccttcggcg ctgtcgtggc tgcgctcctc tccggctgcg
       61 ccccctccga gcaggaggtg gattacgagg cccgcaaggc gctgctcgag cggcagaacc
      121 aggggctccg tgagctgatc gccgaggccg aagccggcac gctcgttccg gccgaccgct
      181 tcctggtggg cgtggacgag tcgatcctga agcaggtgct cgactcgcag ctcccgttcg
      241 agcaccagat cggcgacaag ctcggcatcc ggctggagcg ggccacggtg ctcctgcgcg
      301 acaagtacgg ccacatgacg ctcgagggcg aagtgcaccg cctcgccacg ccggaccgga
      361 agacgaaggt ccgcatctac ggcgggctgg gcgccgtatc ggtcgattcc acctccaacc
      421 ggctcagcgt gaagatcgcc gtcgaccgca tcgtgctcgt cgaggccggc ctgctcgatg
      481 ccgtgctggg cgccaacggc aagaagttgc tgtccgagaa aggtcgggag atcctcgagg
      541 acaagctgcc ggtgctccag gtgccggtgg cgctggccca gaccattcgc gtgcccgcca
      601 tggccgacgg gccgctgcag ctcgactcgc tcagcgtgcc gctcaacctg tcggtcgatc
      661 gggtgctggc cgccgggggc aagctgtggc tcaccatcca tgccgagctg ggcgccgtgc
      721 agggtggcga agaggggctt ggcgtgtcgg tcatcaagaa gaagaagccg aaggcctcgt
      781 gatgcgaacc ccccgcttcc gcctcgtccc cctcctctgc gtcctcgcgc tcgccgcccc
      841 gggttgcgcg cgccgggcct cggaggccga gacacgacaa tggaacggca cgctggcccg
      901 cctgcaggcc gagcaggact cactgcgcgc ccgggccgcc gagctcatca tggccgatcc
      961 gaacctggcc aggctgccgt ccgaccccgt ggtcatccgc gtccccacca gcttcgtgcg
     1021 ggaggtcgtc gagcacctga tgacgagcgt ggtggacaac atcacgctca ggctgtcggg
     1081 catcaaggcg cacgtggcca agaaggtgaa gaagatcgtc accatcggcg agttcaagct
     1141 cgacgtggag gtgcacgagg tcgtcggcaa gctcacgacc gaaaagcctg aagtcgggtt
     1201 cggcgacggg gaaatctcga tgagccttcc cgtcaacgcc accgagggaa cgggcaccgc
     1261 aacgctccac ttccagtggg acggcaagcg cctggccgac cttacctgcg gcgacctgga
     1321 cgtgacgaag acgctgaccg gccaggtcgt gcccgcccgc tacatcatcg ccggcaggat
     1381 gggactcgcc gtcacggaca acaagatcgt ctgcacgccg atcttccccg agacgcgcat
     1441 ccggctccgg gttgagccga ccaaggcggc ctgggacacc gtcaacgccc tgctggccga
     1501 gaagcacggg gtctgcggct gggtcctcga caaggtcaac gtgccgtcga tcctcgaggg
     1561 gatcatccag gagaaggg
//
LOCUS       DASVOD010000534         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_453311, whole genome shotgun sequence.
ACCESSION   DASVOD010000534 DASVOD010000000
VERSION     DASVOD010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453311"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF720_11870"
     CDS             <1..391
                     /locus_tag="VF720_11870"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904759.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7880102.1"
                     /translation="ANYLMTKLAPVFELAYPGPCMHECVLSGRKFKPKGVRTMDIAKR
                     ILDFGMYAPTVYFPLIVEEALMIEPTESETVDTLDRFVEVMKTIAREALEDPDMVKNA
                     PHTTPVRRPDEGKAAREARLRWRPAAN"
     gene            370..828
                     /locus_tag="VF720_11875"
     CDS             370..828
                     /locus_tag="VF720_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880103.1"
                     /translation="MEAGGELTKGVGALAEPLEWRVAPWRRDPGKSALAVFVVFSAIG
                     GAVWFSGGIWFFGLLALLMMWGSIGPYFVTSRFVLDAAGAEIDSPFLKRRRAWSEIKS
                     YYLDRHGATLSPFAGRNWLESYRSVRLLFGEHGEAVRRRLKEQLGEPSRG"
     gene            809..1141
                     /locus_tag="VF720_11880"
     CDS             809..1141
                     /locus_tag="VF720_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880104.1"
                     /translation="MNRPADKPGEPTPADIELLETIAKAVVVRRMTVPAILFLESSRP
                     LSFLGSQFLYFLEPIVRTFIKGDQYRRVAVLMEDRGNVERLLLAIESQESVAQELEKQ
                     RKREGRKP"
     gene            1180..>2180
                     /locus_tag="VF720_11885"
     CDS             1180..>2180
                     /locus_tag="VF720_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEX7880105.1"
                     /translation="MSVLATDCGSTTTKAILIERRNGEYRLIVRGEAPTTVEAPFEDV
                     TRGVLNAIREVEELAGRQILNGEEIMSPQKGDKGVDIYLSTSSAGGGLQMMVTGAVKA
                     MSAESAERAALGAGAIVMDVLAANDGRLPHERIQRIRHLRPDMVLLSGGTDGGDKIKV
                     AEMAELIAAADPKARLGTQYKLPVIYAGNKEAQPIVKQRIGDRTDLEMVDNLRPTLES
                     ENLRPARDAIHHLFMEHVMAHAPGYNKLLTWTPVPVMPTPGAVGLMIETIAKEEEIDV
                     LGVDIGGATTDVFSVFSGIFNRTVSANLGMSYSVSNVLAEAGIDNVMRWVPFKMDAG"
BASE COUNT          400 a          688 c          748 g          344 t
ORIGIN      
        1 cgccaactac ctgatgacga aactcgcgcc ggtcttcgaa ctggcttatc ccggcccatg
       61 catgcacgaa tgtgtcctct cggggcgcaa attcaagccg aagggcgtcc gcacgatgga
      121 catcgccaag cgcattctcg atttcgggat gtacgccccg acggtctatt tcccgctgat
      181 cgtggaagag gccctgatga tcgagccgac cgagtccgag acggtggaca cgctcgaccg
      241 cttcgtcgag gtcatgaaga cgatcgcccg ggaagcgctc gaggatcccg acatggtgaa
      301 gaacgcgccg cacaccactc cggtccgtcg tccggacgag gggaaagcgg cgcgggaggc
      361 aaggctccgg tggaggccgg cggcgaactg acgaaggggg tgggcgcgtt ggccgagccc
      421 ctcgaatggc gggtggcgcc gtggcggcgc gatccgggaa agtccgcgct ggccgtattc
      481 gtggtgtttt cggccatcgg cggggcggtg tggttctccg gggggatctg gttcttcggc
      541 ctcctggcgc tcctcatgat gtggggatcg atcggcccgt atttcgtcac cagccggttc
      601 gtcctggacg ccgccggcgc ggagatcgac tcgccgttcc tgaaacggcg gcgtgcctgg
      661 tcggagatca agtcgtatta cctcgatcgg cacggggcca cgttgtcgcc cttcgccggg
      721 cgaaactggc tggagtcgta ccgttcggtg cgcctcctgt tcggcgaaca cggggaagcc
      781 gtgcggcggc gcctgaagga gcaactgggt gaaccgtccc gcggataagc cgggcgagcc
      841 gacaccggcc gacatcgaac tgctggagac gatcgcgaaa gcggtggtcg ttagaaggat
      901 gaccgttccg gcgattctgt tcctggaatc gagccggccg ttgagtttcc tgggtagcca
      961 gttcctgtac ttcctcgagc cgatcgtccg gacattcatc aagggcgatc agtaccgccg
     1021 ggtggcggtg ttgatggagg accggggaaa cgtggaacgg ctgctgctgg ccatcgagtc
     1081 gcaggaatcg gtggcgcaag agcttgaaaa gcagaggaag agggagggac gtaaaccgtg
     1141 agcaacgaca agcagtcggc gcggatcccg gccgaagtga tgtcggtgct ggccacggac
     1201 tgtggcagca cgaccaccaa ggccatcctg atcgagcgcc ggaacggcga ataccgcctg
     1261 atcgtccgcg gcgaagcgcc gaccacggtc gaggccccct tcgaggacgt gacgcgcggc
     1321 gtgctgaacg ccatccgcga ggtcgaggaa ctggccggtc gccagatcct gaacggcgag
     1381 gagatcatgt cgccccagaa gggggacaag ggagtcgata tctacctctc cacatcctcg
     1441 gccggcggcg gcctccagat gatggtgacc ggcgcggtga aggccatgag cgccgagtcg
     1501 gccgagcgcg ccgcgctggg cgccggcgcc atcgtgatgg acgtgctggc cgccaacgac
     1561 ggccgcctgc cgcacgagcg gatccaacgt atccggcacc tccgccccga catggtgctc
     1621 ctctcgggcg gcaccgacgg cggcgacaag atcaaggtgg ccgagatggc cgagctgatc
     1681 gcggcggccg atcccaaggc ccgcctcggc acgcagtaca agctgccggt catctacgcc
     1741 ggcaacaagg aagcgcagcc gatcgtgaag cagcgcatcg gcgaccggac cgacctcgag
     1801 atggtcgaca acctccggcc gacactcgaa tccgagaacc tgcgccccgc ccgcgacgcc
     1861 atccatcatc tcttcatgga acacgtgatg gcccacgctc ccggctacaa caagctcctc
     1921 acctggacgc cggtgccggt catgccgacg cccggcgccg tcgggctcat gatcgagacg
     1981 atcgcgaagg aagaggagat cgacgtcctg ggtgtcgata tcggcggcgc caccaccgac
     2041 gtcttcagcg tcttcagcgg catcttcaac cgcacggtga gcgccaacct cggcatgtcc
     2101 tactcggtgt cgaacgtcct ggccgaggcc ggcatcgaca acgtgatgcg ctgggtgccg
     2161 ttcaagatgg acgcagggga
//
LOCUS       DASVOD010000535         4306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_453321, whole genome shotgun sequence.
ACCESSION   DASVOD010000535 DASVOD010000000
VERSION     DASVOD010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453321"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VF720_11890"
     CDS             complement(<1..862)
                     /locus_tag="VF720_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015447257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX7880106.1"
                     /translation="MASSDTHRAIDAIWRIEAARVIAGVARIVRDVGLAEDLAHDALL
                     AALEHWPVSGVPANPGAWLTTTSRRRALDHLRHVRQAEAKHEAIGRDLDIRHAWGAFD
                     VDRHLDDDIGDDLLRLVFTACHPVLPTEGRIALTLRLLGGLTTPEIARAFLVPEPTVA
                     QRIVRARKVLADRQIPFDVPRGADLVPRLASVLDVIYLIFNEGYAATSGDDWMRPALC
                     EDALRLGRVVAGLMPGESEVHGLVALMEIQASRARARTTPAGDPILLLEQDRSRWDRL
                     LINRGLAALDE"
     gene            complement(919..1362)
                     /locus_tag="VF720_11895"
     CDS             complement(919..1362)
                     /locus_tag="VF720_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX7880107.1"
                     /translation="MRFMVMVKATKESEAGILPSEELLAAMGNYNEQLMKAGVLLAGE
                     GLQASSKGARVRFSGDKRIVIDGPFAETKELVAGFWILQVKSKEEAVEWVKRCPNPFP
                     GAESEIEIRQIFEADDFGAEFTPELREQEERLRAAAEAKGRADKS"
     gene            complement(1550..2779)
                     /locus_tag="VF720_11900"
     CDS             complement(1550..2779)
                     /locus_tag="VF720_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnH family di-heme enzyme"
                     /protein_id="HEX7880108.1"
                     /translation="MIRRAASALAIPDRARAVLAAAAGLIALLGLTSCGGESTKPAAP
                     TVRELLKLPEHIQEPFIPDYNPISAGKIALGRRLFYDRMLSGNRTQSCASCHRQELGF
                     ADGIPRPFGSTGQMLARNSQGLANVAYNASFTWGNNVLLDLEHQIHVPIEADNPIELG
                     VIDGNRAEVLARFDNDAVYHAMFADAFPDDPGPVTINKIVFALASFLRTMVSGGSAYD
                     RYYAGDATALDESQVRGLRLFNSERFECFHCHSGVNLSISYRDARTTPGTITYPFFNN
                     GLYNVGGSGDYPHGNQGLFEVTQNPDDRGMFRPQGMRNIALTAPYMHDGSIPTLTDVV
                     KHYARGGRLTSSGPDAGDGRLSPLKSGLIRGFEASDEEIADVVHFLESMTDPFFLNNP
                     DFSDPFLEEAAARPRLP"
     gene            complement(2776..3693)
                     /locus_tag="VF720_11905"
     CDS             complement(2776..3693)
                     /locus_tag="VF720_11905"
                     /inference="COORDINATES: protein motif:HMM:NF042462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbnP family copper-binding protein"
                     /protein_id="HEX7880109.1"
                     /translation="MPRFPKLLPMASILAVLLVLSSGCGDDDDPTPRDFTLRFQAVDG
                     GTTIGCGTVMSGFGSDGESTVEVSDLRFYVSNLRFFDASGDPVTIDLDENDFQYSSPE
                     GSVALLDLTGTVAGACGTAELEGTARTNGIITGTYTGNDVTRVTFDVGVPQAVMKKVI
                     ANNTAEGAPSPLAEMHWSWAGAYRHLVMNFAIVDGEGVGGEGFVHVGSTGCGGDGTKA
                     LTDRDECNRINAPAVSLASFDLESDVVALDVRELLEGIALKDGNNAPGCGCHSFTTQA
                     DCAGIFSNLGLTLATGASNAALDEVFKVK"
     gene            complement(3859..>4306)
                     /gene="rsgA"
                     /locus_tag="VF720_11910"
     CDS             complement(3859..>4306)
                     /gene="rsgA"
                     /locus_tag="VF720_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HEX7880110.1"
                     /translation="STLLNRLAGEEIARVQDIREDDRGRHTTTSRNLVVLPGGAILID
                     TPGKRTVILWGGSDGVEQVFDDIAALAASCRFRDCDHDSEPGCAVREALAAGALEASR
                     FQSYRKLQREALHHEMRVDTKVRLAENRRWRAIHRAQRARPDKRTI"
BASE COUNT          742 a         1368 c         1475 g          721 t
ORIGIN      
        1 cctcgtccag ggcggccagg ccacgattga tcaggaggcg atcccagcgg gagcgatcct
       61 gctcgagaag caggatcgga tcacccgccg gcgtcgtacg ggcccgggcg cgcgaggcct
      121 ggatttccat gagggcgacg aggccgtgca cttcggattc gcccggcatg agcccggcca
      181 ccacgcggcc gagccggagg gcatcctcgc aaagcgcggg gcgcatccag tcgtccccgc
      241 tggtggccgc gtacccttcg ttgaagatca ggtagatgac gtcgaggacc gacgccaacc
      301 gggggaccag gtcggcgccg cggggaacgt cgaagggaat ctgcctgtcg gcgaggacct
      361 tcctggcccg cacgatccgc tgggccacgg tcggctcggg caccaggaag gcacgggcga
      421 tctccggggt ggtgaggcca ccgagcaggc gcagcgtgag cgcgatgcga ccttcggtgg
      481 gaaggaccgg atgacaggcc gtgaagacga gccgaaggag atcatccccg atgtcgtcat
      541 ccaggtgacg atcgacgtcg aaggcgcccc aggcgtggcg gatgtcgagg tcgcggccga
      601 tcgcctcgtg tttggcctcg gcctgtctca catgacgaag atgatcgagg gcccggcggc
      661 gggaggtggt cgtgagccag gcgccgggat tggccggcac gcccgaaacg ggccagtgct
      721 cgagcgccgc caggagggca tcatgcgcca ggtcttccgc caggccgaca tcgcgcacga
      781 tcctcgcaac gccggcgatc acccgggcgg cctcgatgcg ccagatggca tcgatggcgc
      841 ggtgcgtgtc ggaggaagcc atggccggca atcacaccat cgggcagcgc ccggcgcaag
      901 ccgggcgcgc ccgtcgggtc aggacttgtc ggcgcggccc ttcgcctcgg cggcggcccg
      961 caggcgctct tcctgctcgc gcagttccgg cgtgaactcg gcgccgaagt cgtcggcctc
     1021 gaagatctgg cggatctcga tctcggactc cgcgccgggg aaaggattgg ggcagcgctt
     1081 gacccactcg acggcctcct ctttcgactt gacctgcagg atccagaatc cggcaacgag
     1141 ttccttcgtt tcggcgaagg ggccgtcaat gacgatgcgc ttgtctccgg aaaacctgac
     1201 cctcgccccc ttcgacgatg cctggagccc ctcgcctgcc aggagcacgc cagccttcat
     1261 cagctgctcg ttgtagttcc ccatcgccgc cagcaattcc tcggacggga ggatgcccgc
     1321 ttcggattcc ttcgttgcct tgaccatgac catgaaacgc attggagact ctccttgatt
     1381 cgtcgtggag gccgatccct ctcccggtcg accgaccggt cgttcgtgcc gtcatgaaca
     1441 cgacgaacga gcgggtcccg gatcgacatg gcaccgacac ttttcttgat ccctataatt
     1501 gactatctca caaatactta caggaggagc tggccggatg ggacccggat caggggagcc
     1561 tggggcgcgc agcggcttcc tcgaggaagg gatccgagaa gtccggattg ttgaggaaga
     1621 acgggtcggt catcgactcg aggaagtgca ccacgtcggc gatttcctcg tcgctggcct
     1681 cgaagccgcg gatgaggccc gacttgagcg gcgagaggcg tccatcgccg gcgtcggggc
     1741 ccgaggacgt aagacggccg cctcgggcgt agtgcttcac gacgtcggtc agcgtcggga
     1801 tgctcccgtc gtgcatgtag ggcgcggtca gggcgatgtt gcgcatcccc tgcgggcgga
     1861 acatcccgcg gtcgtccgga ttctgggtga cctcgaaaag cccctggttg ccgtggggat
     1921 agtcgcccga accgcccacg ttgtagagac cgttgttgaa gaagggatag gtgatcgtac
     1981 cgggagtggt ccgcgcatcg cgataggaaa tcgagaggtt cacgccgctg tggcagtgaa
     2041 agcattcgaa gcgttccgag ttgaagaggc gaagcccccg gacctgcgat tcgtccaggg
     2101 cggtggcgtc acccgcgtag tagcggtcgt aggcactccc gcccgacacc attgtccgga
     2161 ggaaactggc cagcgcgaag acgatcttgt tgatggtgac cggccccgga tcgtcgggaa
     2221 aagcgtcggc gaacatcgcg tgatacacgg cgtcgttgtc gaaacgggcc agcacttcgg
     2281 cccggtttcc gtcgatcacg cccagctcaa tcgggttgtc ggcctcgatc gggacatgga
     2341 tctggtgctc gagatcgagg aggacgttgt tgccccaggt aaaggaagcg ttgtacgcca
     2401 cattggccag accctggctg ttccgggcca gcatctgccc cgttgaaccg aacgggcgcg
     2461 gaatgccgtc ggcgaaaccg agttcctgcc tgtggcacga ggcgcacgac tgcgtgcgat
     2521 ttcccgacag catccggtcg tagaagaggc gccggccgag ggcgatcttc ccggccgaga
     2581 tcgggttgta atcggggatg aacggctcct ggatatgctc gggcagcttc agaagctcgc
     2641 gcaccgtggg ggccgccggc ttcgtcgatt cgccgccgca ggacgtgagg ccgagcaggg
     2701 caatcaggcc cgctgccgcc gcaaggacgg ctcgggctcg gtcgggaata gcgagtgccg
     2761 atgcggcgcg gcggatcact tgaccttgaa aacctcatcc agcgccgcgt tcgaggcacc
     2821 ggtggccagc gtcagaccaa ggttggagaa gatgccggcg cagtcggcct gggtggtgaa
     2881 ggaatggcag ccgcaaccgg gggcattgtt gccgtccttc aatgcaatcc cctcgagcag
     2941 ttcacggaca tcgagggcca ccacgtcgga ctcgaggtcg aaggacgcca gtgacaccgc
     3001 gggtgcgttg atgcggttgc attcgtcacg gtcggtgagg gccttggtgc cgtccccgcc
     3061 gcagccggtg ctgcccacat gaacgaaccc ctcccctccc actccttcgc cgtcgacaat
     3121 ggcgaagttc atcacgaggt gacggtaagc accggcccag gaccagtgca tctcggccag
     3181 cggcgatggg gctccttcgg cggtgttgtt ggcgatgacc ttcttcatca ccgcctgcgg
     3241 cacgcccacg tcgaacgtga cacgcgtgac gtcgttcccc gtataggtgc cggtgatgat
     3301 gccgttcgtc cgggccgttc cttcgagttc cgcggttccg caggcgccgg caacggtgcc
     3361 ggtgaggtct aagagcgcca ccgagccctc cgggctcgag tactggaagt cattttcgtc
     3421 gaggtcgatc gtgaccgggt cgccgctggc gtcgaagaag cggagattgc tgacatagaa
     3481 acggaggtca ctcacttcaa ccgtcgattc cccatcggac ccgaacccgg acatcaccgt
     3541 gccgcagccg atggtggtac cgccatcgac cgcctggaat cggagcgtga agtcccgggg
     3601 cgtcggatcg tcgtcatcgc cgcagccgga ggaaagcacg aggaggacgg cgaggatcga
     3661 ggccatcggg agcagcttcg ggaaacgggg catggggtct cctggggtgg gcaacgcctg
     3721 atgccggtgt tgccagcgca tggcagccgg tatgcgagcg agatcaagtt tcgcgcaacc
     3781 tccgacgagg ggtcaagatc gcctcattgc acccgggtgg taacacaccc gggagggacg
     3841 tttccggtta ctcgcgcctc agatcgtccg cttgtcgggg cgggcgcgct gggcccggtg
     3901 gatggcccgc caccggcggt tctcggccag gcgaaccttc gtgtccacgc gcatctcgtg
     3961 atgcagcgcc tcccgctgca gtttccgata gctctggaac cgcgatgcct cgagagctcc
     4021 agccgccaac gcttcccgca ccgcacaacc cggttcggag tcgtggtcgc agtcgcggaa
     4081 gcggcacgaa gccgccagcg ccgcgatgtc gtcgaacacc tgctccacgc cgtcactacc
     4141 gccccagagg atgaccgtgc gcttgccggg cgtgtcgatc aaaatggcgc caccgggcag
     4201 aacgacgagg ttccgcgacg tggtggtgtg gcgcccgcga tcgtcctcgc gaatatcctg
     4261 cacgcgggcg atctcctccc ccgccagccg gttgaggagc gtggac
//
LOCUS       DASVOD010000536         4138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_399326, whole genome shotgun sequence.
ACCESSION   DASVOD010000536 DASVOD010000000
VERSION     DASVOD010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399326"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..831
                     /gene="surE"
                     /locus_tag="VF720_11915"
     CDS             85..831
                     /gene="surE"
                     /locus_tag="VF720_11915"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX7880111.1"
                     /translation="MKILVTNDDGINAPGIKALARAFRALGDVIVVAPDSEKSASGHA
                     LTLQHPLRIHQVGEGEYATTGTPTDCVLLAVHALLEGKHPDLVVSGVNTGPNMGDDVT
                     YSGTVSAAFEGTLLGIPSMAISLATFDPVPYDPAADIALRIAKRFLERGLPPKTLLNV
                     NVPPGALGSHKGVRLTKLGHRVFTDIIAPRVDPRGKPYYWVAGTAKWAPEAGTDIEAI
                     QQGYVSVTPLNMDMTDFKLLAELEAWGLES"
     gene            1074..1150
                     /locus_tag="VF720_11920"
     tRNA            1074..1150
                     /locus_tag="VF720_11920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1108..1110,aa:Pro,seq:cgg)
     gene            1209..2312
                     /locus_tag="VF720_11925"
     CDS             1209..2312
                     /locus_tag="VF720_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEX7880112.1"
                     /translation="MSEPKGDTTGAGSPKAPVPFKDLLAQALEGGKSSRRITQKTIDR
                     LLAADDFDDTEFELFVDLARDLEIEIHDYPETANDIDKPTPELDSLKLYLADISRYPL
                     LKAEEEISVGKNIQEGTTPHIRQSNRKRMILSNLRLVVKIARAYSGRGVPIQDLIEEG
                     NLGLITAVDRFDYKRGFRFSTYGSWWIKQSIIRGIATQSHTVRIPLHVIQLVNRYIAT
                     ESRIRHKTRSQPTLEEVALAMGEPPKRIQRLKQLIDAIKGLDYETSWEALGALSEVDV
                     LKPPPSLESQIDAILENERILKLMGRLSPREETVLRIRYGFNDGESHTLSATGEQIGV
                     SRERVRQIEKRALQKMKRYIELTEQGLKIDQVE"
     gene            2346..2873
                     /locus_tag="VF720_11930"
     CDS             2346..2873
                     /locus_tag="VF720_11930"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017327840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX7880113.1"
                     /translation="MDLKKVIRSIPDFPKKGIMFRDVTPLFTNPPAFREAVDQLDAAF
                     PDRSITAVAAVESRGFIFGGALAYKRGIKFVPVRKAGKLPHATLRTEYALEYGSGVLE
                     VHADALSAGDKVVLIDDLIATGGTAEASVRLLRELGAEVVGAGFLIELAFLPWREKLA
                     GIPTTCLISYADENP"
     gene            3065..>4138
                     /locus_tag="VF720_11935"
     CDS             3065..>4138
                     /locus_tag="VF720_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880114.1"
                     /translation="MSIFITVSPRRFRRPRASMGRRPVIVAVVAAICFLAVAAILSAA
                     PAHSAWLDGGTPVSTAVYAQQNPVIAEDDAGGVIVAWHDTRAGHYDIYAQRITATGDV
                     AWNAEGVPVAAATGAQKFPVIVSDGAGGALLAWKDDRTGNYDIRAQRLGPDGGKAWGP
                     DGIVICGAADAQQDVEIVEDGQGGAILVWRDFRAAGVPQLFAQRVNSAGVSQWAFNGV
                     PIGTTKQHYSVSLAPDGAHGAFVAWYDWRNGNNFDIYAQRLNGAGQPLWLSSGVPVVT
                     ATGNQMYPRLTLDGLGGVFVGWYDARNGGYDIYVQRLAPNGATATGWPAGGRGVCLAP
                     NDQFEPVMTPDGAGGAFVAWYDNR"
BASE COUNT          730 a         1398 c         1346 g          664 t
ORIGIN      
        1 atccagtcgc cccgaccaca tcagcgcccg gaagcccttg agcttccggg cgcaagcttt
       61 tcccgttgaa attcgaggct tcgagtgaag atcctggtga ccaacgacga cggcatcaac
      121 gcccccggca tcaaggcgct cgccagggcc ttccgcgccc ttggcgacgt catcgtggtg
      181 gcccccgaca gcgaaaagag cgcctccggc cacgccctca cgctccagca tcccctccgg
      241 atccaccagg tcggagaagg ggagtacgcc accaccggca cccccaccga ctgcgtcctg
      301 ctggcggttc acgccctgct cgaggggaag caccccgacc tggtcgtatc cggtgtcaat
      361 acaggtccga acatgggcga tgacgtcacc tactcgggta ccgtgtccgc cgccttcgaa
      421 gggacgctgc tcggcatccc ctcgatggcc atcagcctcg cgaccttcga ccccgtgccg
      481 tacgatccgg ccgccgacat cgccttgcgc atcgcgaagc gattcctcga aagaggtctg
      541 cctcccaaga cactgctcaa cgtgaatgtg cctcccggag ctttgggaag ccacaaaggc
      601 gtccgcctca ccaagctcgg ccaccgcgtc ttcaccgaca tcatcgcccc gcgtgtcgac
      661 ccgcgcggca agccgtacta ctgggtggcg gggacggcca agtgggcgcc ggaggcgggg
      721 accgacatcg aggcgatcca gcagggatac gtgtcagtca cgccgctgaa catggacatg
      781 acggatttca agctgctggc ggagctcgaa gcgtgggggc tggagtcgta ggtcgaggtc
      841 cggctccgtc gattccgatc aacaggttcc ggaacctgtt gactgtttct ctcacaggtc
      901 cacccaactc gttttgaaaa cgagccgggg gcggcatggt ccagcagcag caggccaaag
      961 tgaatcctgg cctcggccgc accatctccc ttgatcggcc gccctcaacg agttatagtc
     1021 ccccgcttcg aacgcccgcc ggtcctgatg tccggtggcc gttcgcatga ggtcggggcg
     1081 tagcgcagtc tggtagcgca cctggttcgg gaccaggggg tcggaggttc aaatcctctc
     1141 gccccgacca atttttcgtc cgtgcgtccc ccgcacggcg agtggatgtg gaccccggga
     1201 gcttttccat gagcgaaccg aagggcgaca ccaccggcgc cggaagcccc aaggcgcccg
     1261 ttccgttcaa ggatctcctt gcccaggcac tcgagggggg caagtcgagc cggcggatca
     1321 cgcagaagac catcgaccgc ctcctggctg ccgatgattt cgacgatacg gagttcgagc
     1381 tgttcgtcga cctggcgcgc gatctcgaga tcgagatcca tgactatccg gaaacggcca
     1441 atgacatcga caagcccacc ccggaactcg acagcctgaa gctctacctg gccgacatct
     1501 cccgctatcc gctcctcaag gcggaagagg aaatctcggt cgggaagaac atccaggaag
     1561 ggaccacccc gcacatccgc cagtcgaacc ggaagcggat gatcctgtcg aacctgcgcc
     1621 tggtcgtgaa aatcgcgcgc gcctattcgg ggcgcggggt gccgatccag gacctgatcg
     1681 aagaggggaa cctggggctc atcacggccg tcgatcgctt cgactacaag cgcggcttcc
     1741 gcttctcgac gtacggctcg tggtggatca agcagtcgat catccgcggg atcgccacgc
     1801 agagccacac cgttcgtatc ccgctgcacg tcatccagct cgtcaaccgc tacatcgcca
     1861 ccgagagccg gatccgccac aagacgcgga gccagccgac gctggaagaa gtggcgctgg
     1921 cgatggggga gccgccgaag cgcatccagc ggctgaagca gctcatcgac gccatcaagg
     1981 ggctcgacta cgaaacctcg tgggaggcgc tgggcgccct ctccgaagtg gacgtgctga
     2041 aaccgccgcc gtcgctcgag agccagatcg acgccatcct cgagaacgag cggatcctga
     2101 aactgatggg gcgcctctcg ccgcgcgagg aaacggtgct gcgcatccgg tacggattca
     2161 acgacggcga gtcccacacg ctgtcggcca cgggtgagca gatcggcgtg agccgggagc
     2221 gggttcggca gatcgagaag cgggcgctgc agaagatgaa acgctatatc gaactcaccg
     2281 agcagggact gaaaatcgat caggtcgagt gacctgtcat tgaatcaagg agaccgaccg
     2341 tggccatcga cctgaagaaa gtgatccgct cgatcccgga tttccccaag aaggggatca
     2401 tgttccgcga cgtgacgccc ctcttcacca acccgccggc cttccgcgag gcggtggacc
     2461 agctcgacgc ggcgtttccc gatcgctcga tcacggcggt ggcggcggtg gagagccggg
     2521 ggttcatctt cggcggggcg ctggcctaca agcgggggat caagttcgtg ccggtccgca
     2581 aggcgggcaa gctcccccac gccaccctcc gcaccgagta cgcgctggag tacggctccg
     2641 gggtgctcga ggtgcacgcc gacgccctgt cggcggggga caaggtggtc ctgatcgatg
     2701 acctgatcgc cacgggtggc acggccgagg ccagcgtgcg gcttctccgt gagctcgggg
     2761 ccgaggtggt cggcgcgggc ttcctgatcg agctggcgtt cctcccgtgg cgggagaaac
     2821 tggccgggat ccccacgacc tgcctgattt cgtacgccga cgagaacccc tgatcggagc
     2881 tttcgagacg ttggggcccc gggtcgggtg tgtggccccg gacggcccga ttccggccct
     2941 cccgccgggt caaatcgccc gatttttgcg aaaagctcta aagcccccgc ccgggggtgg
     3001 tcgataaccg gaaggccgcc atcggcatgg ccgtcgatca ccagtctgca acgggggttg
     3061 catcatgtcc atcttcatca cggtttcgcc ccgccgcttc cgtcgtcccc gcgcctccat
     3121 ggggagacgt cccgtcatcg tggccgtcgt ggcagccatc tgtttcctcg ccgtggcggc
     3181 aatcctttcc gccgcacccg cgcattccgc gtggctggat ggcggcactc cggtatcgac
     3241 cgccgtctac gcgcagcaga atccggtaat cgccgaggat gatgccggcg gcgtgatcgt
     3301 ggcctggcat gacacgcggg ccggccacta cgacatctat gcccagcgga tcaccgcaac
     3361 gggcgacgtg gcctggaacg ccgagggcgt gcccgtggcg gccgcaacgg gcgcgcagaa
     3421 gtttcccgtc atcgtttcgg acggcgccgg tggcgccctc ctggcgtgga aggacgatcg
     3481 caccggcaac tacgacatcc gcgctcagcg gctcgggccc gacggcggca aggcgtgggg
     3541 cccggacggc atcgtgatct gcggcgcggc cgacgcccag caggacgtgg agatcgtgga
     3601 agacggacag ggtggtgcga tcctcgtgtg gcgcgacttc cgcgccgcgg gcgtgccgca
     3661 gctctttgcc cagcgggtga acagcgccgg cgtgtcgcag tgggccttca acggcgtgcc
     3721 gatcgggaca accaagcagc attacagcgt gtcattggcg cccgacggcg cgcacggcgc
     3781 gttcgtggcc tggtacgact ggcggaacgg caacaacttc gatatctacg cccagcgcct
     3841 gaacggtgcg gggcagcccc tgtggctctc gagcggcgtt cccgtcgtga cggccaccgg
     3901 caaccagatg tatccccgcc tgacgctgga tggcctgggt ggcgtcttcg tgggatggta
     3961 tgacgcccgc aacggcggct acgacatcta cgtgcagcgc ctcgcgccga acggcgccac
     4021 cgccacggga tggcccgccg gcggccgcgg ggtgtgcctc gcgcccaacg accagttcga
     4081 acccgtcatg acgcccgacg gcgcgggcgg cgccttcgtg gcctggtacg acaatcgc
//
LOCUS       DASVOD010000537         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_296619, whole genome shotgun sequence.
ACCESSION   DASVOD010000537 DASVOD010000000
VERSION     DASVOD010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296619"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF720_11940"
     CDS             complement(<1..278)
                     /locus_tag="VF720_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880115.1"
                     /translation="MADRRVLFRQIGSVSLIGMVAQVAFLAANFVLQRHLSTGDFGRA
                     SLSNYVINLATYLGLLGANTAILRAMPRRELSRTNWPALLGRVSMTALP"
     gene            complement(271..996)
                     /locus_tag="VF720_11945"
     CDS             complement(271..996)
                     /locus_tag="VF720_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7880116.1"
                     /translation="MATRPSETTPGAMRRFYEDVAERTLDRKTSRGIEYRNRTILARL
                     GSPGRLLEIGPGEGWLVGAALRSGHQVVAADLSRRWLSRLPDRNSSSLLRAQGDAARL
                     PFPEATFDRVVAAEVLEHLPDPSATIREARRVLKPDGRFVATVPYRETLRRLRCPHCG
                     DEFEPNGHLHSFDEGKLAGLFTDAGLVPGFRLIAPTRFSREIWRLANWPPLLPALHAL
                     DRATIGSQRVSDTWLLLEGTRVG"
     gene            complement(1032..1715)
                     /locus_tag="VF720_11950"
     CDS             complement(1032..1715)
                     /locus_tag="VF720_11950"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX7880117.1"
                     /translation="MTFSARLLMPSSLVAMLAAVVVLVAARVEAAVLPEAAPLLDRLE
                     GAIGPLKDFQADFVQVRHVDLTDENIEARGRLRFLAPESFRLDYREPDADVLTMRGDS
                     LLVYFPALAQAQRHRIDREDATRNIFLLFASKKGELADRFDVSLAPPGPGGAALRLQP
                     LAGVLDYPIQEIVVRLQSKTNLPEELFFREEGGDTVVFRLADVRTNRKLKSSDFVFVP
                     PPGTEILEP"
     gene            complement(1681..2499)
                     /locus_tag="VF720_11955"
     CDS             complement(1681..2499)
                     /locus_tag="VF720_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880118.1"
                     /translation="MALFVGGCALGGGGSGFRLPEGDPPPDSLVTWINSQALSTGSVK
                     GSGKLRVVTPEYPGGRQLDMSLVARRPDLVRMRGRVGILASIFDFLADADSLHFYLPR
                     DHVELAQANVPGADLMSIVASRELVEAILPPPIVIGGAAGDIMLEKGKDAWIVTRTRT
                     LNGAPRLHRSVYDAERLKLVEMSIRPAGAAVDSPEVMITYDRHAWTGQAWFPGRIRLE
                     LPGETRSVTLTFETFEPNPATSPDVFTWSVPAGTRRLSPEEMTDDFLRSAPDAE"
     gene            2748..>3549
                     /locus_tag="VF720_11960"
     CDS             2748..>3549
                     /locus_tag="VF720_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880119.1"
                     /translation="MIELLRTRLARPCLIALTAVAALLLPSAAPVAASDAGTLLPAGL
                     SRLADALADSIDAITARETVMVTDVRENGIRIDRGAAAGLRPYSRLTLLPADLPPWTA
                     EIADLEDAAAWLRPVDPDRAAPPAGTEARIRVDLSRIAVDRITASGDGAHRWADAWLD
                     QLADRLQSRAALATIVMPAYPDTSEWRESARQAGGDAGVLLSLTESAGKWTATAQAAS
                     LRRARTIGTTRATVRAPSATGPKSAPPDWPLEPPPGLLAPMSVAHLDGA"
BASE COUNT          574 a         1194 c         1223 g          558 t
ORIGIN      
        1 gggagtgccg tcatggacac gcggccgagg agtgccggcc agttcgtccg ggagagttcc
       61 cggcgtggca tggccctgag aattgcggtg ttggccccga ggaggccgag ataggtggcc
      121 aggttgatca cgtagttgga caggctggcc cgcccgaagt cgccggtgga gaggtggcgt
      181 tgcaggacga agttcgcggc gaggaacgcc acctgcgcca ccatgccgat caagcttacc
      241 gacccgatct gtcggaacag gacccgccgg tcagccaacc cgagtcccct cgaggagcaa
      301 ccaggtgtcg ctcacccgct ggctgccgat ggtggcgcgg tcgagggcgt gcagcgccgg
      361 caggagtggc ggccagttgg ccagtcgcca gatttcgcgg ctgaagcgcg tcggggcgat
      421 caggcgaaat cccgggacga gcccggcgtc ggtgaagagt cccgcgagtt tcccttcgtc
      481 gaacgaatgc aggtgaccgt tcggttcgaa ctcgtcgccg cagtgcgggc acctcaggcg
      541 acgaagtgtt tcccggtacg gaacggtggc cacgaaacga ccgtccggtt tcaggacgcg
      601 cctcgcctcg cggatcgtgg ccgagggatc gggaaggtgt tcgaggactt cggcggccac
      661 tacccggtcg aaggtggcct cggggaaggg aaggcgcgcg gcgtcaccct gcgcgcgcag
      721 gaggctcgaa ctgttcctgt cgggaaggcg tgagagccat cggcgggaga gatcggcggc
      781 caccacctgg tgaccactcc ggagcgccgc gccgacgagc catccctcgc cgggtccgat
      841 ctcgagaagc cgtccggggc ttccgagacg cgcgaggatg gtgcgattgc gatactcgat
      901 cccccggctc gtcttcctgt cgagggtgcg ttcggccacg tcttcgtaga accgccgcat
      961 cgcgccggga gtggtttcgg acggtcgggt cgccatcggg gcgggatagt agcagccgcg
     1021 ccgccgcccc gtcagggttc gaggatctcg gtccccggcg gcggcacgaa aacgaagtcg
     1081 gacgacttga gcttccggtt ggtgcgcacg tcggccaggc ggaagacgac ggtgtcgccc
     1141 ccttcctcgc gaaagaaaag ttcctcggga agattggtct tcgactgcag gcgtaccacg
     1201 atctcctgga tcgggtaatc gagaacgccg gccagtggtt gaagccggag cgcggcaccg
     1261 ccggggccgg gcggcgccag gctcacgtcg aatcggtcgg caagctctcc cttcttcgag
     1321 gcgaagagga ggaagatgtt ccgcgtggca tcttcacgat cgatgcggtg acgctgtgcc
     1381 tgggcgagcg ccgggaaata gacgagcagc gaatcgcccc gcatggtgag cacatcggca
     1441 tccggctcac ggtaatcgag gcggaatgat tccggagcca ggaacctgag ccgaccacgg
     1501 gcctcgatgt tctcgtcggt gagatcgacg tgccgcacct ggacgaagtc ggcctggaag
     1561 tccttgagcg gcccgatggc gccttcgagg cggtcgagca acggggccgc ctccggcagg
     1621 accgcggcct ccacacgcgc ggcgaccagc acgaccaccg ccgccagcat ggcaaccagg
     1681 ctactcggca tcaggagccg agcggagaaa gtcatcggtc atctcctcgg gagagagtcg
     1741 tcgcgtcccg gccgggacgg accatgtgaa gacatcgggg gaagtcgcgg gattgggttc
     1801 gaacgtctcg aacgtcaggg tgacactccg ggtttcgccc gggagttcca gcctgattcg
     1861 gcccgggaac caggcctggc ccgtccaggc atggcgatcg tacgtgatca tgacctccgg
     1921 actgtcgacc gccgcgccgg ccgggcggat cgacatctcg accagcttga ggcgctcggc
     1981 gtcgtacacg ctccggtgga gtcgcggcgc gccgttgagc gtgcgtgtcc tcgtcacgat
     2041 ccatgcgtcc ttccccttct ccagcatgat atcgcctgcg gcgccaccga tcacgatcgg
     2101 cggcggcagg atcgcctcca ccagttcgcg ggacgccacg atcgacatga ggtcggcgcc
     2161 gggcacgttg gcctgggcga gttcgacatg atcccgcgga aggtaaaagt ggaggctgtc
     2221 cgcatccgcg agaaagtcga agatcgaggc gagtatcccc acgcggcccc gcatgcggac
     2281 gaggtccggt cggcgcgcca cgagactcat gtcgagctgc cggccacctg ggtactcggg
     2341 tgtcaccacc cgcaattttc cggagccttt taccgagccg gtcgagagcg cctggctgtt
     2401 gatccatgtc acgagcgaat cgggcggcgg atctccttcc ggcaggcgga atccggagcc
     2461 accgccgccg agcgcgcatc ctccgacgaa gagcgccatg agcagcacgg agacgtgtgt
     2521 aaaaaaaacc ggggtcggaa ccttccgttg ccgcgccggg tggacgttca tatatttcct
     2581 tgagcgcttc atgtgagtga agggcatccc ccgaagccgg ggacgggttc attcccgtcc
     2641 ccggtttcac ttccggggcg cttgattgac cgggcatgat agcccaccta ctatttccgt
     2701 ttcgcggtgc cgcccccggg tggccgactc ccccgggacg gaagggaatg atcgaactgc
     2761 tccgcacgcg acttgcccgc ccctgcctga tcgccctcac cgccgttgcc gctcttcttc
     2821 tcccgtccgc cgcgcctgtc gctgcgtcgg acgcgggcac gctcctcccg gccggcctct
     2881 cccggctggc cgacgcgctg gccgattcga tcgacgccat cacggcccgg gaaacggtga
     2941 tggtgaccga tgtccgggag aacggaattc gtatcgatcg gggcgcggcc gccggtctcc
     3001 gcccctactc gcgcctcacg ctcctccccg ccgacctccc gccatggacg gccgagatcg
     3061 ccgacctgga ggatgccgcc gcctggctca ggccggtcga cccggatcgc gcggcgccgc
     3121 ctgcgggaac cgaagcccgc atccgcgtcg acctgtcgcg catcgccgtc gaccgcatca
     3181 ccgcatccgg tgacggcgcg catcggtggg cggacgcgtg gctcgatcaa ctggccgatc
     3241 gactccaatc ccgggccgcg ctggccacga tcgtaatgcc cgcctatccc gatacgagcg
     3301 agtggcgcga gtcggcgcgt caggccggag gagatgcggg tgtcctgctt tcccttaccg
     3361 agtcggcggg aaagtggacg gccacagccc aggccgcttc gctgcgacgc gcgcgaacga
     3421 tcggcacgac ccgcgccacg gtacgggcgc cttccgccac gggcccgaag tcggcgccgc
     3481 ccgactggcc gctcgaaccg ccgcccggcc tgctcgcccc catgtccgtc gcccacctcg
     3541 acggcgcga
//
LOCUS       DASVOD010000538         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_296670, whole genome shotgun sequence.
ACCESSION   DASVOD010000538 DASVOD010000000
VERSION     DASVOD010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296670"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..>1619)
                     /locus_tag="VF720_11965"
     CDS             complement(120..>1619)
                     /locus_tag="VF720_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7880120.1"
                     /translation="GGDLILSIDGELQQVVAERLDAAVARCKARGGYVIVIEPSTGDI
                     LAMANSPSFDPTWYERPESIAHNRNRLVADGIEPGSTIKAITSAAALEDGAFRPNTPI
                     DCHMGHWMLQGKPITDHEGFGVIPFIDTFVHSSNVAMAQVGLKLGPQKMYHYFRKFGF
                     AERTGIDLPGETAGTLKRPENWSGRTAATVAFGYEIRVSPIQMAMAYAALANKGVLMK
                     PRLVRATVDADGHIEERKPVEVRRAVTEKTAATMLDFMVKTVAEGTGRKGAVDWCTVG
                     GKTGTARKYDAKAGGYVSKHYASFVGVAPIEKPALLCYAVIDEPTGEIYGGSTAAPLF
                     KEILEASARLKHPVLRPGYRTIPVKAAPVGPDPVLASRERKVKLGPAVPLAEAVAETI
                     AVAGELASYQAEVARAAADTNAAAPETIVEALGVAPDLVGLAFRNATARASDAGYLPR
                     PVGETGVVVRQFPAPGATMTSLHRGTILLFFGKAPELASREPRQVHDGR"
BASE COUNT          237 a          575 c          551 g          256 t
ORIGIN      
        1 gcccggtccg ggccacgaag aggtcacccg gggcggtcgt cctcgaatcg aacgacacgc
       61 cggtgatcga agccggaaga gcgcccgcaa gccgggcgcc aggaaggagc gcgaggagct
      121 cagcgcccgt catgcacctg ccgcggctca cgggaagcca gctcgggggc cttgccgaag
      181 aaaaggagga tcgttccgcg atggagcgag gtcatcgtgg cgccaggcgc cgggaactgc
      241 cggacgacca cgccggtctc gccgacgggt cgcggaagat agcccgcatc gctcgcacgc
      301 gcggtggcgt tccggaacgc cagccccacc aggtcgggag ccacgccgag cgcctcgacg
      361 atcgtttcgg gcgcggcggc attcgtgtcg gcggcggccc gggccacctc ggcctggtag
      421 ctggccagtt ccccggccac ggcaatggtt tcggccaccg cctcggcgag tggcaccgcc
      481 ggccccagct tcaccttccg ctccctcgag gccagcaccg ggtccggccc gaccggcgca
      541 gccttgaccg ggatcgtccg gtacccggga cgcagcaccg gatgcttgag gcgcgcggag
      601 gcttcgagga tttccttgaa gagcggcgcg gcggtcgagc cgccgtagat ttcgccggtc
      661 ggctcatcga tcaccgcgta gcagaggagc gcgggctttt cgatcggcgc cacgccgacg
      721 aacgaggcgt agtgcttcga tacgtagcca cccgctttcg cgtcgtactt gcgcgccgtg
      781 ccggtcttgc cgcccaccgt gcaccagtcc acggcgccct tccttccggt tccctccgcc
      841 accgtcttca ccatgaagtc gagcatcgtg gccgccgtct tctcggtgac cgcgcgacgc
      901 acctccaccg gcttccgctc ctcgatgtgg ccgtccgcat cgacggtggc gcgcaccagc
      961 cgcggcttca tcagcacgcc cttgttggcc agcgcggcgt aggccatggc catctggatc
     1021 ggggacacgc ggatctcata gccgaacgcc accgtggcgg cggtgcgccc cgaccagttt
     1081 tccgggcgct tgagcgtgcc cgccgtctcg cccgggaggt cgatgccggt acgctcggcg
     1141 aacccgaact tccggaagta gtggtacatc ttctgcggcc ccagcttgag gccaacctgg
     1201 gccatggcca cgttcgagga gtggacgaac gtgtcgatga acggaatcac gccgaacccc
     1261 tcgtggtcgg tgatcggctt cccctggagc atccagtgcc ccatgtggca gtcgattggc
     1321 gtgttcgggc ggaaggcgcc gtcttccagc gcggccgccg aggtgatcgc cttgatcgtc
     1381 gaccccggct cgatgccgtc ggccaccagc cggttgcggt tatgggcgat cgattcgggc
     1441 cgttcgtacc aggtcggatc gaacgagggg gagttggcca tcgccaggat gtcgccggtc
     1501 gagggctcga tcacgatcac gtagcccccc ctggccttgc atcgcgccac cgcggcgtcg
     1561 agccgctcgg ccaccacctg ctggagctcg ccgtcgatcg acaggatgag gtcgccgcc
//
LOCUS       DASVOD010000539         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_244283, whole genome shotgun sequence.
ACCESSION   DASVOD010000539 DASVOD010000000
VERSION     DASVOD010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244283"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="VF720_11970"
     CDS             complement(<1..1762)
                     /locus_tag="VF720_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880121.1"
                     /translation="MPDTRRAIAIVLVGLVVLVSDVMARDRASGDWILDERTTSAGVT
                     FRYVPPAAEVVPAPAIAGADRKTIEVAGAGLAPGGPGTWQVPVHTAQILLPEGSDWSL
                     DFTPHPGARVTDAWFPRVTRNERFVGKRLDDKPIDTPPTATADGITPEAAVTGTVEIF
                     RGRRLLLLTLAPLQFDPIDGSADLIESIDVTVRFHRTSGATSRADQEQVTEDFATGPF
                     RDLVLNPDVDGTDRDTPLRSELIPSGTAMAGRPEVYFDDAPAWIKLGVTTNGIHRVTA
                     SALAGTGASPASIDPATIRMFAGPGTALSEAVDEADVPAWGEPGGFRELAILVKEAGA
                     PNGRFDDADTLLFYGLAVDNFGTTFSPGGTSEWLENENTGTNTYWLTWGGTFPEAPLR
                     MVVDPVVSGGTPVTTVLERTHVEQNDPSLYDPLPREEGIRWEKWWWQQIEDTGVGSLW
                     EIPLPDIDTTRPLDLRIRWWGSNIPDFDFSEPTQQHFLRVSVNSAEEVGVSWGGGGNG
                     ISRYDMNLANQPARAVNQVTARVINFPNAPLRIDQILLAWMEFTWWHRLNVPTSVQSL
                     AFEGSSAAPGPVSFDVSGSEA"
BASE COUNT          291 a          658 c          618 g          300 t
ORIGIN      
        1 ccgcctcgct gccgctcacg tcgaagctca ccggccccgg agccgccgag ctcccctcga
       61 acgccagcga ctggaccgac gtcggcacgt tcagccgatg ccaccaggtg aactccatcc
      121 acgccagcag gatctggtcg atgcggagcg gggcgttcgg aaagttgatg acccgggccg
      181 tcacctggtt cacggcccgc gccggctggt tggcaaggtt catgtcgtag cgggaaatgc
      241 cgtttccccc acctccccag ctgaccccca cctcttccgc ggaattgacc gacacgcgga
      301 ggaagtgctg ctgcgtcggc tccgaaaagt cgaaatcggg aatgttcgat ccccaccagc
      361 ggatccggag gtcgagcgga cgcgtcgtat cgatgtcggg cagcggaatc tcccagagag
      421 aaccgacgcc ggtatcctcg atctgctgcc accaccactt ttcccagcgg atcccctctt
      481 ccctgggaag ggggtcgtag agcgaggggt cgttctgctc gacgtgcgtc ctctcgagga
      541 cagtcgtgac cggcgtgccg cccgatacca ccggatccac caccatccgg agcggcgcct
      601 ccgggaacgt ccccccccaa gtcagccagt aggtgttcgt gccggtgttc tcgttctcca
      661 gccactcgct cgtcccgccc ggtgaaaacg tcgtcccgaa gttgtccacc gccaggccgt
      721 aaaagagaag cgtgtcggcg tcgtcgaagc ggccgttcgg cgcgcccgct tccttgacga
      781 gaatcgccag ctcgcggaag ccgccgggct cgccccaggc cggcacatcc gcttcatcca
      841 ccgcctcgct gagcgccgtc cccggacccg cgaacatccg gatcgttgcc gggtcgatcg
      901 aggccggcga ggcccccgtg cccgccagcg ccgaggccgt cacccggtgg atgccgttcg
      961 tcgtgacccc cagcttgatc caggccgggg cgtcgtcgaa gtagacctcc ggacgaccgg
     1021 ccatggccgt gccgctcggg atgagctcgg agcggagcgg cgtgtcgcga tcggtgccgt
     1081 ccacgtccgg gttcaacacc aggtcgcgga atgggccggt ggcaaagtct tcggttacct
     1141 gttcctgatc cgcacgcgaa gtcgcgcccg acgtacggtg aaaccgcacg gtcacgtcga
     1201 tcgactcgat gaggtcggcg ctgccgtcga tcgggtcaaa ctggagcggc gccagcgtga
     1261 ggagcaggag tcggcgcccg cggaagatct caaccgtgcc ggtgaccgcg gcttccggcg
     1321 tgatgccgtc cgcggtggcc gtcggtggcg tatcgatcgg cttgtcgtcg aggcgcttgc
     1381 cgacgaatcg ctcgttccgc gtgacgcggg gaaaccaggc atccgtgacc cgtgcgcccg
     1441 gatgcggcgt gaagtcgagc gaccagtccg acccttcggg cagcaggatc tgcgcggtgt
     1501 gcaccggcac ctgccaggtg cccgggccgc ccggcgcgag gcccgcgcca gcgacctcga
     1561 tcgtcttccg gtcggccccc gcgatggccg gcgccggaac gacctcggcc gcgggtggca
     1621 cgtagcggaa cgtcacgccg gcggaggtgg tcctttcgtc gaggatccag tcaccggatg
     1681 cccggtcgcg ggccatgaca tccgagacca ggacaacgag tccgacgagg acgatcgcaa
     1741 tggcccgtcg cgtgtccggc atcgggtttc ctcagcgcag tcggacgatc ggctcgacga
     1801 tgtcgatctc ttctccgcca tcggcgggtt gggccgccac cttgaccagg tagacgccgt
     1861 tcgacac
//
LOCUS       DASVOD010000540         7797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_192284, whole genome shotgun sequence.
ACCESSION   DASVOD010000540 DASVOD010000000
VERSION     DASVOD010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192284"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VF720_11975"
     CDS             complement(<1..923)
                     /locus_tag="VF720_11975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019867036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX7880122.1"
                     /translation="MNGGLWLNLAVFMGVLLLLARPLGNVMADVYEGRTPAWLRWLGP
                     FERGLYRLGGVRTNETMSWKRYAAAFLASQVVGLVLLFVLQLLQEVLPLNPAGLAAPP
                     WHLALNTAISFVTNTNWQSYGGETTLSYLVQSLGLTVQNFVSAGAGMAVMVALARGFS
                     RRKSGEIGNYWVDLTRGVVFILLPLAIVVSVVLVAQGVVQTWGPYLATQPIDGHAQLI
                     ARGPAAAQVAIKQLGTNGGGFFNVNSAHPLENPTPLTNLVEMFSILLIPVALAHTWGR
                     LIGSTRQGWALLLAMTILFVPCAVGVMVAEQS"
     gene            complement(920..1012)
                     /gene="kdpF"
                     /locus_tag="VF720_11980"
     CDS             complement(920..1012)
                     /gene="kdpF"
                     /locus_tag="VF720_11980"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX7880123.1"
                     /translation="MTLITIAGAVLFLLLLVYLFVALLDPERFQ"
     gene            complement(1232..1723)
                     /locus_tag="VF720_11985"
     CDS             complement(1232..1723)
                     /locus_tag="VF720_11985"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEX7880124.1"
                     /translation="MSAGTLTSIVYQPKNRPYAADDRFDDFIRVPVEAIEVLEDVGLA
                     DDQKARKGSVRQVNILSADWLASLTPLGYRTKPGEMGEQLIVSGFRVEDLMTGDRFRI
                     GEAVVLEITKPRTGCSRLEHSQGKPLREFQPAIGMLARVITGGRIAVGDPVVALRAVS
                     ETA"
     gene            complement(1764..2822)
                     /gene="selD"
                     /locus_tag="VF720_11990"
     CDS             complement(1764..2822)
                     /gene="selD"
                     /locus_tag="VF720_11990"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEX7880125.1"
                     /translation="MTDDIRLTQLAKRAGCAAKHPPGYLLPLLGTLPQLTDPNVLVGS
                     STADDAAVYKLTDDLALVLTTDFFTPIVDRPYDFGQVAAANSLSDVYAMGGRPTCALN
                     IVGFPESELDPMVLGDILRGAADKAREAGIDIVGGHTIRSDEPIFGLAVVGTIHPDHV
                     LTNARAKPGDALILTKPIGLGIITTAAKQGGDHLNAIGEAVRIMATLNRTGAEVLTTT
                     GIHALTDITGFGLLGHLRNIVAASGVTARVWFDHVPLIPAAVQYINEGIAPGGTHANW
                     RFLADDWVEWGSDLTKEEQLLLCDAQTSGGLLASVRADDAETLLGALHAAGVADARVI
                     GRVVEPSSGRIVVSRSET"
     gene            complement(3030..3590)
                     /locus_tag="VF720_11995"
     CDS             complement(3030..3590)
                     /locus_tag="VF720_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880126.1"
                     /translation="MRLATKLSSVLLASALVLTSGTAMAAHLTIIDTESDVTVQPDGN
                     FEGGFGSSQNGQHDWSFGGTWIALQSATTSGSGIIYFVDPNSGDLEDIYEVSWNWDGG
                     LATIRGRFESDANGVLVSGRTIPNGFPTVNATPGNVDVLGLFRDPSTGDPVSLPSHLT
                     MLVTFDYAPVPTEAATWTRIKTTLGN"
     gene            complement(3725..4762)
                     /locus_tag="VF720_12000"
     CDS             complement(3725..4762)
                     /locus_tag="VF720_12000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX7880127.1"
                     /translation="MISPLNISLALLPVLLFLAALVFLDSFKLTPLRSVVMSIVMGAV
                     VGMGAYGINTWLHTSMGVDAEYLRHVLGPIVEETMKSAWLVWLVGSKRVGFLVDSAIH
                     GFAVGAGFAVLENIYYLLAIRDASPALWLIRGFGTALMHGSATAIVAILGKGLSDRHP
                     RGFPPVFLPALAAGIVIHAGFNVLVLPPMVMTLVVLLVFPVLIYMVFQRSERVTQEWL
                     GLNLDTDLEMLAAMTSGEIGDSKVGQYLMSLGDRFSKDVVADMYCLLQLNLELSILAK
                     GVLMARSAGLDVPMTDDLRDQLREVRHLERSIGPTGRLAMKPIMRLSSRDLWQVYMLS
                     QKKSPRETPAG"
     gene            complement(4777..7758)
                     /locus_tag="VF720_12005"
     CDS             complement(4777..7758)
                     /locus_tag="VF720_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880128.1"
                     /translation="MPRSKVVLPFHFLLLGALASISVPAGHAHDCLETDDLRLVYTDA
                     TLGWLAPYTARCYENSMAFHKKLWGYKPSEKVTVIMDDIGDYSNAGVWVGPRNSMVLH
                     VAPANNVFETGPSNERMYFTMNHEAVHVVALDQGEGRDIFFRDVFGGKVRETAEYPES
                     ILWSHLTLPRRSAPRWYHEGIAVFFETWMSGGYGRAQGPYDEMVFRSMVRDSTRIYDP
                     MGLESEGTRVDFQVGVNSYLYGTRFMSWLALEYGPESLVQWVGRGPDSRAEYRGQFKK
                     VYHRSLDDAWAEWISFEKGFQAANLDSLRKYPTTGYHDVTERALGSVSPTCFDKASRT
                     LYVAVQYPGVVAHIAAIDVDTGEMRRLVDVKGPALYFVSSMALDDSTGTLFYTTDNND
                     WRDLCALDLSTGKCRRLMRDVRIGDLAFNPVDRSLWGVRHFNALSSLVRMMPPYDEFE
                     LVSSWPYGEDLYDVDISSDGKWLSASHAEISGRQSLRLFEVARLVAADTTSIELHDFG
                     ASIPTSFVFSPDDRYLFGSSYYTGVSNIWRYDLAADSMEIVSNAETGFFRPRPIGEDS
                     LLVFRYSGNGFVPAWITPGVLTDVSPITFLGERIADKHPVVRTWALPPPTGVPLDSLT
                     TYQGKYKPLGHLGLANWYPIVQGYKEYTGLGARMEWSDPLSVNTISVSAAYSGGGNLP
                     DDERLHLGMEWVHGSLTAAFNLNRADFYDLFGPTKTSRKGRGLSLRWKKSLIYDAPRN
                     MELTTGAAWFSGLERHPLFQNVPTSPNFSQLVSGWARIEDKNQRSSIGAADTEKGYRW
                     QGLALLNSVRLESNEGDISWKTNPQFIGALDLGTPIFIPNSSVWLRTSAGWCPGDRDD
                     PFANFFFGGFGNNWVDHGDPKRYREIEGFPGIELNAAGGTNFAKAMVDWNLPAIRFRR
                     AGVPSFFASWVRASLFAGGLWTNLDEDALSSRALDVGGQVDLRMTALSRHNLTLSFGY
                     ARAFVEDLPDDDEYMVSLKIL"
BASE COUNT         1418 a         2549 c         2568 g         1262 t
ORIGIN      
        1 gactgctcgg ccaccatgac gccgacggcg cacggaacga acaggatcgt catggccagg
       61 aggagcgccc atccctgcct ggtcgatccg atcagccgac cccacgtgtg ggccagcgcc
      121 accggaatga gcaggatgga gaacatctcg acgaggtttg tcagcggcgt cgggttctcg
      181 agcggatgcg ccgagttcac gttgaaaaac ccgccgccgt tcgtccccag ttgcttgatg
      241 gccacctggg ccgccgccgg cccacgcgcg atgagttgcg catgcccgtc gatcggctgg
      301 gttgcgagat acggccccca cgtctgcacc accccctgcg ccacgagcac gaccgacacc
      361 acgatcgcca gcggcaggag tatgaagacg acgccccgcg tgaggtccac ccagtagttg
      421 ccgatctccc cgctcttccg ccgcgagaaa ccgcgcgcca gggccaccat caccgccatc
      481 cccgccccgg ccgacacgaa gttctgcacg gtgaggccga gagactgcac gaggtagctg
      541 agcgtcgtct cgccgccgta gctctgccag ttggtgttgg tcacgaagct gatggccgtg
      601 ttgagcgcca ggtgccacgg cggcgccgca aggcccgccg ggttgagcgg caatacctcc
      661 tgcaggagct ggaggacgaa cagcagcacg aggcccacca cctgggaggc caggaaggcc
      721 gccgcgtatc gcttccagct catcgtctcg ttggtacgca cgcctcccaa gcggtacagc
      781 ccgcgttcaa acggtccgag ccagcgcagc caggcgggcg tccgtccctc atacacatcc
      841 gccatcacgt tgccgagcgg tcgcgccagc aggagcaaca cacccatgaa gactgccaga
      901 ttgagccaga gcccgccgtt cactggaatc gctccgggtc gaggagggcc acgaacagat
      961 agacgagcag caggagaaag agcaccgcac cggcaatcgt gataagtgtc atggctattc
     1021 gaaccaggcg gtcacgcgga cactgtcgac gccaggcaca ctcccagggc cacgcccgct
     1081 cccccggcac ttctcaggat cctgcccacg acactcctcc cgtccccggc ggtagtgccg
     1141 gcggacacag ggaatcgtcc cgcgggatcg cgctaagagg gtgctaattg aaacgggcgg
     1201 gatgttaaga aagcgtgaag agtccccgcc gtcaggcggt ttcggacacc gcgcgcagcg
     1261 cgaccaccgg atcacccacg gcaatgcgcc cgccggtgat cacgcgcgcc agcatcccga
     1321 tggccggctg aaattcgcgc agcggctttc cctgcgagtg ctcgaggcgg ctgcagccgg
     1381 ttcggggctt ggtgatctcg aggacgacgg cctcgccgat ccggaagcga tcgcccgtca
     1441 tcaaatcttc cacccggaag ccggacacga tgagctgctc ccccatctcg ccgggcttcg
     1501 tgcgatagcc gagcggcgtg agcgaagcca gccagtcggc ggagaggatg ttcacctggc
     1561 gcaccgagcc cttccgcgcc ttctggtcgt cggccaaccc cacgtcctcg aggacttcga
     1621 tcgcctccac cgggacgcgg atgaaatcat cgaagcggtc atcggcggca taggggcgat
     1681 tcttcggctg gtacacgatg ctggtcagcg ttccggcgga catcacgact cctttgcgaa
     1741 cgatgtcaat tctcctaacg agatcaagtt tccgagcggc tcacgacgat ccggcccgac
     1801 gacggctcca ccacccgccc gatcacgcgc gcatcggcca cgcccgccgc atgcagcgcc
     1861 ccgaggagcg tttcggcgtc gtcggcccgc acggaggcca ggaggccgcc ggacgtctgc
     1921 gcatcgcaca ggaggagctg ctcctccttc gtgaggtcgc taccccactc cacccaatcg
     1981 tccgccagga agcgccaatt ggcgtgcgtg cctcccggcg cgattccttc gttgatgtac
     2041 tgcaccgccg ccgggatcaa cggcacgtga tcgaaccaga cccgcgcggt tacaccgctc
     2101 gccgccacga tgttccggag atgtccgagc agtccaaaac ccgtgatgtc ggtcagggca
     2161 tggatccccg tggtcgtgag gacctccgca ccggtgcgat tgagcgtggc catgatgcgg
     2221 acggcttcgc cgatcgcatt cagatggtca cccccctgct tggccgccgt cgtgatgatg
     2281 ccgaggccga tcggcttggt gaggatcagc gcatcgcccg gtttcgcgcg ggcattcgtc
     2341 agcacgtggt cgggatgaat ggtgccgacg accgccaatc cgaagatcgg ctcgtcactg
     2401 cgtatggtgt ggccacccac gatgtcgatg ccggcctcgc gagccttgtc ggcggcgccg
     2461 cgcaggatgt ctcccaggac catcggatcg agttccgact cgggaaagcc cacgatgttg
     2521 agcgcgcagg tcggccgccc gcccatggca tacacgtccg acagcgaatt ggccgccgcc
     2581 acctgcccga agtcgtacgg cctgtcgacg atcggcgtga agaagtcggt agtcaggacc
     2641 agggccaggt cgtccgtgag cttgtagacg gcggcgtcgt cggccgttga gctgccgacg
     2701 aggacgttgg gatcggtgag ctgcgggagc gtgcccagca acgggagcag gtagcccggc
     2761 gggtgtttcg cggcgcagcc ggcgcgcttg gcgagctggg tgaggcggat atcgtcggtc
     2821 atgggccttg gatacgcggg ccggctccgc ggttcgcgcg ggccggcccg gtcagcgact
     2881 cggcggaagc agatcgtccc gtggacgatc cacacggttg acggtcgtcg gggatcatga
     2941 tgggatgata atccgggacg ggcccggccg gaaatgacac ggggcccgcg accgtgagat
     3001 cgcgggcccc gctgatttcg ttcggagact cagttgccca gcgtggtctt gatgcgcgtc
     3061 caggtggccg cctcggtggg caccggggcg taatcgaacg tgacgagcat cgtcaggtgg
     3121 ctggggaggg agaccggatc accggtcgag ggatcgcgga acaggcccag cacgtcgacg
     3181 ttgcccggag tggcattcac cgtcgggaac ccgttgggaa tcgtgcggcc ggaaacgagc
     3241 acgccgttcg catcggactc gaagcgaccc ctgatcgtgg cgaggccgcc atcccagttc
     3301 cacgaaacct catagatgtc ctcgaggtcg ccggagttcg ggtcgacgaa gtaaatgatg
     3361 ccgctgccgg aggtcgtggc ggattggagg gcgatccagg ttccaccgaa ggaccagtcg
     3421 tgctgcccgt tctgcgagga gccgaacccc ccctcgaaat tgccgtccgg ctggaccgtg
     3481 acgtcggatt cagtgtcgat gatcgtgaga tgtgccgcca tcgcggtgcc ggacgtgaga
     3541 acgagggcgg aggcgagcag aacggacgag agcttggttg ccaggcgcat ggtgtttccc
     3601 cctgtagttg cggccacgtt gtcccccggg tagtccccgg agcgagggaa tctacaccaa
     3661 attcgcgcgt cactaaagcc aaattcgggt gggtcgtagt caggccgccg gcgccacgcg
     3721 ggagtcaccc tgccggtgtc tcccgcggcg acttcttctg cgacagcatg tagacctgcc
     3781 acagatcccg gctcgacagc cgcatgatcg gcttcatggc caggcgcccc gtcggcccga
     3841 tggagcgctc gaggtgccgc acctcgcgaa gctggtcgcg caggtcgtcg gtcatcggca
     3901 cgtccaggcc ggccgaccgg gccataagca cccccttggc caggatcgac agctcgaggt
     3961 tgagctgcag caggcagtac atgtcggcca caacgtcctt cgagaagcga tccccgaggc
     4021 tcatcaggta ctggcccacc ttggagtcgc cgatctcgcc gctggtcatg gccgccagca
     4081 tctcgaggtc ggtgtccagg ttgaggccga gccactcctg cgtgacccgc tcgctccgct
     4141 ggaacaccat gtagatgagc accggaaaga cgagcaggac gaccagcgtc atgaccatcg
     4201 gcggaaggac gagcacgttg aatccggcgt ggatgacgat gcccgcggcg agggccggca
     4261 ggaacacggg cgggaaacca cggggatggc ggtccgacag ccccttgccc aggatggcca
     4321 cgatggcggt ggcggagccg tgcatgagcg ccgtgccaaa cccccggatg agccagagtg
     4381 cggggctggc gtcgcggatg gccagcaggt agtagatgtt ttccagcacg gcgaagccgg
     4441 ccccgacggc aaatccgtga atggccgagt cgacgaggaa gcccacccgc ttggaaccga
     4501 ccagccagac gagccaggcc gacttcatcg tttcttccac gatcggtccg agtacatggc
     4561 gcaggtactc ggcatccaca cccatcgacg tgtgaagcca tgtgttgatg ccgtacgcac
     4621 ccatgccgac caccgccccc atgacgatcg acatcacgac cgaccggagc ggcgtgagct
     4681 tgaagctgtc caggaatacc agcgcggcga ggaacaggag caccgggagg agtgccaggc
     4741 tgatgttcag cggtgagatc atcggcgaac cgccactcag aggatcttga gcgacaccat
     4801 gtactcgtcg tcatccggca ggtcttccac gaaggcccgc gcgtagccga aagagagtgt
     4861 caggttgtga cgggacaggg ccgtcatccg gaggtccacc tggccgccga cgtcgagggc
     4921 ccggctggac agggcgtctt cgtcgaggtt tgtccagagc ccgccggcga agagcgacgc
     4981 ccggacccac gaagcgaaga aagaggggac gcccgcccgg cgaaagcgta tggccggcag
     5041 attccagtcg accatcgcct tggcgaagtt ggtgccgccg gcggcattca gctcgatgcc
     5101 cgggaaccct tcgatctccc ggtaacgctt cggatcgcca tgatccaccc agttgttgcc
     5161 gaagccgccg aagaagaagt tggcgaacgg gtcatcccgg tcgccgggac accagccggc
     5221 gctggtccgc agccagaccg aggaattcgg gatgaagatc ggcgtgccga ggtcgagcgc
     5281 gccgatgaat tgcgggttcg tcttccagct gatgtctccc tcgttcgatt ccagccggac
     5341 ggagttgagg agcgccagac cctgccatcg ataccccttc tcggtgtcgg cggctccgat
     5401 cgaggagcgc tggttcttgt cctcgatgcg cgcccagccg ctcaccagct ggctgaaatt
     5461 gggcgatgtg ggaacgttct ggaacaacgg gtggcgttcc agcccggaaa accaggcggc
     5521 gccggtcgtc agctccatgt tccgtggcgc gtcgtagatc aggctcttct tccagcgcag
     5581 ggagagccct cgacccttcc ggctcgtctt ggtcgggccg aacaggtcgt agaagtcggc
     5641 gcgattcagg ttgaatgccg ctgtcaggct gccgtgcacc cattccatgc caaggtgcag
     5701 ccgttcgtcg tcggggaggt tgccaccccc cgagtacgcc gcgctcacgc tgatcgtgtt
     5761 caccgacagg ggatccgacc actccatgcg cgcaccgagc cccgtgtact ccttgtaccc
     5821 ctgcacgatc gggtaccagt tcgcgaggcc caggtgtccg agcggtttgt atttcccctg
     5881 gtatgtggtc agcgaatcga gcggcacgcc ggtgggcggc ggcaaggccc acgtgcggac
     5941 caccgggtgc ttgtcggcga tccgttcgcc gaggaacgtg atcggactca cgtcggtcag
     6001 gacgcccggc gtaatccagg ccggcacgaa cccatttccc gaatatcgga agacgagcag
     6061 cgaatcctcc ccgatcggtc gggggcggaa gaacccggtt tcggcattcg acacgatctc
     6121 catcgagtcg gcggccaggt catagcgcca tatgttcgat acaccggtgt agtacgaact
     6181 gccgaacagg tagcggtcgt cgggcgagaa gacgaagctg gtcgggatgg acgcgccgaa
     6241 gtcgtgcagc tcgatcgagg tcgtgtcggc ggcaaccagg cgggccacct cgaagagccg
     6301 gagcgactgg cgccccgaga tctcggcgtg actggccgag agccacttcc catcggacga
     6361 aatgtcgacg tcgtacaggt cctcgccata cggccacgat gagacgagct cgaattcatc
     6421 gtacgggggc atcatccgca ccaggctcga gagcgcgttg aagtgccgca ctccccagag
     6481 ggatcggtcg acgggattga aggccaggtc gccgatccgc acgtccctca tgagtcggcg
     6541 gcacttcccc gtggacaggt ccagcgcgca caggtcccgc cagtcgttgt tgtcggtcgt
     6601 gtagaagagc gtgcccgtcg agtcgtccag tgccatcgac gagacgaaat agagggccgg
     6661 ccccttcacg tcgacgaggc ggcgcatctc gccggtatcg acatcgatgg cggcgatgtg
     6721 ggccaccacg cccgggtact gcacggccac gtacagcgtc cggctggcct tgtcgaagca
     6781 ggtgggcgat accgacccca gggcccgctc cgtcacgtcg tggtagcccg tggtgggata
     6841 cttgcgcagc gagtcgaggt tggccgcctg gaagcccttt tcgaaggaga tccactccgc
     6901 ccaggcatcg tccagcgaac ggtgatacac cttcttgaac tgcccgcggt actccgccct
     6961 ggagtcggga ccgcgcccca cccattgcac cagtgactcc gggccgtact cgagcgccag
     7021 ccagctcata aaacgcgtcc catacaggta cgagttgacc cccacctgga agtccacccg
     7081 cgtcccctcc gattcgagcc ccatcgggtc gtagatgcgc gtggaatcgc gcaccatcga
     7141 ccggaagacc atctcgtcgt agggcccctg ggcacggcca tagccgcccg acatccacgt
     7201 ctcgaagaag acggcgatcc cctcgtggta ccagcggggc gccgaccgtc gcggcagcgt
     7261 gagatggctc cagaggatcg attcgggata ctccgccgtt tcgcggacct ttccgccgaa
     7321 gacgtcgcgg aagaagatgt cccgcccctc tccctggtcc agtgccacca cgtgaacggc
     7381 ctcgtggttc atcgtgaagt acatccgctc gttgctcggg cccgtctcga aaacgttgtt
     7441 ggccggggcc acatggagca ccatgctgtt ccgcggcccc acccagacgc cggcgttcga
     7501 gtagtcgccg atgtcgtcca tgatgaccgt gaccttctcg ctcggcttgt acccccacag
     7561 tttcttgtgg aaggccatcg agttttcgta gcagcgggcc gtgtaaggcg ccagccagcc
     7621 gagcgtggcg tcggtgtaaa cgaggcgcag gtcgtccgtc tcaaggcagt cgtgggcatg
     7681 cccggccgga acggaaatcg acgccagggc gccgagaagc aggaaatgga aagggagaac
     7741 gacttttgat cgcggcatgg gcggcggcct ccgcgcaggt gtgcgaaatg gagaggt
//
LOCUS       DASVOD010000541         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_975534, whole genome shotgun sequence.
ACCESSION   DASVOD010000541 DASVOD010000000
VERSION     DASVOD010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975534"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF720_12010"
     CDS             complement(<1..921)
                     /locus_tag="VF720_12010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7880129.1"
                     /translation="MRLALVVFAAALAVRMVYLLEFSRSVFFDVPILDAAWHAAWADR
                     IAHGHWLDGAPYFRAPLYTWFLAVLDAAIGTGPWIARIAQAILGSLAAALTALTAERV
                     WNRSGLAAGLVVAFYGPIVFSTGELLHEALLVPLLAAWLYLTVFANDERNDPDARSLP
                     WFGSGFLLGLAAVARPSALILAPLAVGLALFPTRRTRFVLPILAGLLLPIVPVTAINY
                     VSSGDFVPIASQGGINFYAGNNGAADGRHVVIPELAGLGGWEDFEPQVKAIAEKVSGR
                     PLGPAGVSDWWAARGWSWIRHHPGAAAGLWF"
     gene            complement(962..2137)
                     /locus_tag="VF720_12015"
     CDS             complement(962..2137)
                     /locus_tag="VF720_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7880130.1"
                     /translation="MAFSKFAGFDFYEMDGLLTDEEKMARDTVREFVDQEVLPIIDRH
                     FENATYPHELTPKMADLGIFGANLPEKYGCAGMSNVAYGLIMQELERGDSGLRSAASV
                     QGSLVMYPIYAYGSEAQKDHWLPKLATGKAIGCFGLTEPDFGSNPAGMKTVAKKDGND
                     WIINGAKMWITNGTVSDIAVVWARTDDGIQGFLVEKGTPGFSAPEQKFKMSLRASVTS
                     ELVLSDVRVPDSARLPNVRGLKGPLGCLTQARYGIAWGALGAAMGCLHAAVEYGKDRV
                     QFDRPIAGFQLTQAKLADMFTEITKGQLLCLQLGRLKDAGTMKPEQVSMAKRNNVAIA
                     LDIAREARGILGANGITLEYPVIRHMLNLESVKTYEGTHEIHTLVLGEALTGIAAYR"
     gene            complement(2260..>2859)
                     /locus_tag="VF720_12020"
     CDS             complement(2260..>2859)
                     /locus_tag="VF720_12020"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEX7880131.1"
                     /translation="KTDLVILGRRGKYDVRIDVARAGNGAPWSMTPVITELGGKTLES
                     PEGLTFVANVKTDLENVARDRAALIRKNPNKPDYLGAASCASCHPSEHATWSGSRHAH
                     AWDPMVSTKNTLNPLCLHCHTVGFMEPNGYVIGSDDKPLQGVGCESCHGPGSRHVADP
                     RGNPLGVVGEAKCRTCHTSGQTPDFDFNEFWPKIRHGKP"
BASE COUNT          476 a          945 c          970 g          468 t
ORIGIN      
        1 gaaccacaac cccgcggcgg cgccggggtg atgacggatc catgaccacc cccgggccgc
       61 ccaccagtcg gagactcccg cggggccgag cgggcgcccc gaaactttct cggcgatggc
      121 ctttacctga ggctcgaagt cctcccatcc cccgaggccg gccagctccg ggatgacgac
      181 atgccggccg tccgcggcac cgttgtttcc cgcatagaaa ttgatccctc cctggctggc
      241 gatcggcacg aaatcgccgg agctcacgta gttgatggcc gtgaccggta cgatcggaag
      301 aaggaggccg gccaggatcg gcaggacaaa gcgtgtccgg cgcgtcggga acaacgccag
      361 gccgaccgcc agcggtgcaa ggatgagcgc gctcggccgg gcgacggccg cgaggcccag
      421 caggaacccg gagccgaacc atgggagcga gcgggcatcc ggatcgttgc gctcgtcgtt
      481 ggcgaagacg gtgaggtaga gccacgccgc caggagcggg acgagcagcg cttcgtgcag
      541 cagctctccg gttgagaaga cgatggggcc gtagaaggcc accaccaagc cggccgcgag
      601 tccggatcga ttccacacgc gctccgccgt gagcgcagtc aaggcggcgg ccaggcttcc
      661 caggatcgcc tgcgcgatcc gggcgatcca cggacccgtc ccgatcgcgg cgtcgagcac
      721 cgccaggaac caggtgtaga gtggcgcccg gaaataggga gcgccgtcca gccagtgccc
      781 atgggcgatc cggtccgccc acgccgcgtg ccaggccgca tcgaggatcg gcacatcgaa
      841 gaagaccgaa cgcgagaatt cgaggagata gaccatccgc acggcgaggg cggccgcgaa
      901 aacgacgagg gcgagccgca tggcccgccc ccggtcggaa gccactgtgg tttcggtcgg
      961 ctcagcggta ggcggcaatg cccgtcagcg cctcgcccag caccagtgtg tggatctcgt
     1021 gcgttccctc gtacgtcttc accgactcga ggttcagcat gtggcggatg acggggtact
     1081 cgagggtgat gccgttggcg ccgaggatgc cgcgcgcctc gcgggcgatg tcgagcgcga
     1141 tggccacgtt gttccgcttc gccatcgaca cctgttcggg cttcatcgtg ccggcatcct
     1201 tgagacgacc gagctgcagg cagaggagct gccccttggt gatctcggtg aacatatcgg
     1261 ccagcttggc ctgcgtgagc tggaagccgg cgatcgggcg gtcgaactgc acgcggtcct
     1321 tgccgtattc cacggccgcg tggaggcagc ccatggcggc gccaagcgcg ccccaggcga
     1381 tgccgtagcg ggcctgcgtc aggcatccga gcggcccctt gaggccgcgc acgttcggaa
     1441 gccttgctga atcgggcacc cgcacgtcac tcagcaccag ctccgacgtg accgaggcgc
     1501 gcagcgacat cttgaacttc tgctcgggcg ccgagaagcc gggggtgcct ttctcgacca
     1561 ggaatccctg gatcccgtca tcggtccgcg cccacaccac ggcaatgtcc gaaacggtgc
     1621 cgttggtgat ccacatcttg gccccgttga tgatccagtc gttgccgtcc ttttttgcca
     1681 ccgtcttcat gccggccggg ttgctgccga agtcgggctc ggtaaggccg aagcagccga
     1741 tggccttgcc ggtggccagc ttcgggagcc agtggtcttt ctgcgcctcg ctgccgtagg
     1801 cgtagatcgg gtacatgacc agcgacccct gcaccgaggc cgccgaccgg aggcccgaat
     1861 cacctcgctc cagttcctgc atgatcagcc cgtacgccac gttgctcatc cccgcgcagc
     1921 cgtacttctc ggggagattg gccccgaaaa tgccgaggtc ggccatcttg ggggtgagct
     1981 cgtgcgggta ggtggcgttc tcgaagtgcc ggtcgatgat cggcagcacc tcctggtcga
     2041 cgaactcgcg aacggtgtcg cgggccatct tttcctcgtc ggtcaggaga ccgtccatct
     2101 cgtagaaatc gaaaccggca aacttgctga aggccatgag gaactccggg tggcagggtg
     2161 cgcgaaagca caaggtgact caattgaacg gcggcaagag tagcgccctg accggtgatg
     2221 gatcaagcgc gaggcgcggc catccgccgc cggcgcggtt cagggctttc cgtgccggat
     2281 cttcggccag aactcgttga agtcgaagtc cggcgtctgc cccgacgtgt gacaggtccg
     2341 gcatttggcc tcaccgacca ctccaagggg gttcccgcgc ggatcggcca cgtggcggct
     2401 tcccgggcca tggcacgact cgcagccgac cccctggagc ggcttgtcgt ccgaaccgat
     2461 gacgtagccg ttcggctcca tgaaccccac ggtgtggcaa tgcaggcaca gcggattcag
     2521 cgtgttcttc gtcgacacca tcgggtccca ggcatgcgcg tggcgcgacc cggaccaggt
     2581 ggcgtgctcg gaagggtggc agctggcgca cgaggcggcg ccgagatagt cgggcttgtt
     2641 cgggttcttc ctgatgaggg cggccctgtc gcgggccacg ttttcgaggt cggtcttcac
     2701 gttggccacg aacgtgagcc cttcgggact ctccagcgtc ttgccgccca gctcggtgat
     2761 cacgggcgtc atcgaccagg gggcgccgtt tcccgcgcgc gccacgtcga tgcgcacgtc
     2821 gtacttgccg cgccgtccca gtatgacgag gtcggtctt
//
LOCUS       DASVOD010000542         2559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_660614, whole genome shotgun sequence.
ACCESSION   DASVOD010000542 DASVOD010000000
VERSION     DASVOD010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660614"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VF720_12025"
     CDS             complement(<1..680)
                     /locus_tag="VF720_12025"
                     /inference="COORDINATES: protein motif:HMM:NF015912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
                     /protein_id="HEX7880132.1"
                     /translation="MTKPKAAATKKAAGAAAKKESGPIKELKSLALKQGYVTQEQLDS
                     FISSDAGGEDLIAEMENAYTALGDMNIDVFDSEEEALAKMRKAKKTKGSKADPKAAAV
                     VAPVRYDDPVRMYLREMGKVPLLDREGEVTIAKRIESAEHRIMDALFKTAPSVKELQA
                     RAVLLAKEKMKIEEFVHVDFGGPEGGEAATKKLRAKVLTAVKNIAKNQPELAKLRQQG
                     MRAMSEKTR"
     gene            complement(677..2434)
                     /gene="dnaG"
                     /locus_tag="VF720_12030"
     CDS             complement(677..2434)
                     /gene="dnaG"
                     /locus_tag="VF720_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX7880133.1"
                     /translation="MRYPQDLIDRVREATDIVALVGEQVRLTRAGRSYKGLCPFHTEK
                     TPSFHVNPDRQIYHCFGCGAGGNAISFLIEHAKLTFPEAVRTLAERAGIPLPTRTEDA
                     GADLEIGRIHEALALAQEFFRERLNHREVGRPAREYLESRDITMETAESFGLGFAPDG
                     WANFFDVASKRFTVEILSRAGLVVPKDAGGGYDRFRNRLMVPIASSSGRVIAFGGRTL
                     GDDEAKYINSPESPVYQKGQLLFGLAMARDAIRRLDEAILVEGYFDHLRVFAAGFTNV
                     VAVAGTAFTSAQATLLRRHAANVLLVFDGDSAGLNAAWKASGLCLAAGLAPRFVILPE
                     GEDPDSLVRRDGPGAFRVALDEALDLVGFTAARLIPKLGKEEALRRLVDQVRESPDPI
                     RRRLLVQEAADRLRFDEGVLVKAVESKRPLGMPRPAGDMTNRPAPSLPAELPKVEKQL
                     LAILIDQPELLEGLGALDENDFDDPRSREIFRQLLLQSGSTGLFHLLMEAGRDEEWGQ
                     VLPQLAFIPTHEGQTAAHDCAVALRVKRLDGLMSLKLRELASQERSGDPAAKAEILRQ
                     IKELSDEKHSLATGAMTKS"
BASE COUNT          377 a          871 c          841 g          470 t
ORIGIN      
        1 cgggtcttct cggacatggc gcgcatcccc tgctggcgaa gcttggccag ttcaggctgg
       61 ttcttggcga tgttcttgac cgccgtgagc accttggccc gcagcttctt ggtggccgcc
      121 tcgccccctt cggggccgcc gaagtcgacg tggacgaact cctcgatctt catcttctcc
      181 ttggccagga ggacggcgcg ggcctggagt tccttcaccg acggagccgt cttgaagagc
      241 gcgtccatga tgcgatgctc ggcgctctcg atcctcttgg cgatggtcac ttcgccttca
      301 cggtcgagca gcggcacctt ccccatctcg cgcaggtaca tgcggacggg gtcgtcgtaa
      361 cggaccggcg ccacgacggc ggcggccttc gggtcggcct tcgaaccctt ggtcttcttg
      421 gccttccgca tcttcgcgag cgcttcttcc tcgctgtcga agacgtcgat gttcatgtcg
      481 ccgagcgccg tgtaggcatt ttccatctcg gcaatcaggt cctcgccccc ggcatcgctc
      541 gagatgaacg agtcgagctg ctcctgggtg acgtacccct gcttcaaggc cagcgatttc
      601 aattccttga tcggacccga ttcctttttc gcggccgcgc ccgccgcctt cttcgtcgct
      661 gccgccttcg gtttggtcat gacttggtca tcgctcctgt ggccagagaa tgtttctcgt
      721 ctgacagctc tttaatctgt cgcaggatct cggccttggc ggccggatcc ccgcttcttt
      781 cctgtgatgc cagctccctc agctttagac tcatcagccc gtccagacgc ttcacccgaa
      841 gggccacggc gcagtcgtgc gccgcggtct gaccctcgtg cgtcgggatg aacgccagct
      901 ggggaagcac ctgcccccac tcctcgtcgc gccccgcctc catcaggagg tggaaaagcc
      961 ccgtactccc cgactgcagc aggagttgcc ggaagatctc ccggctccgg gggtcgtcga
     1021 agtcgttttc gtccagggct ccgagcccct ccagcagttc cggctggtcg atcaggatcg
     1081 ccagcaattg cttttcgacc ttgggaagct ccgccggtaa cgacggggcc gggcgattcg
     1141 tcatgtcacc cgccggacgg ggcattccga gcggccgctt cgactccacg gcctttacca
     1201 gaaccccctc gtcgaaccgg agtcggtcgg cggcttcctg aacgaggaga cgccggcgga
     1261 tcgggtcggg cgactcgcgg acctggtcga cgaggcgccg gagggcctct tccttgccga
     1321 gcttcgggat gaggcgggcc gcggtgaagc cgaccaggtc cagggcctcg tcgagggcga
     1381 cccggaaggc gccaggaccg tcacggcgca ccaggctgtc cgggtcttct ccctcgggga
     1441 ggatgacgaa cctgggcgcg aggcccgccg ccaggcagag ccccgaggct ttccaggcgg
     1501 cgttgaggcc ggccgagtcg ccgtcgaaaa cgagcagcac gttcgcggca tggcggcgga
     1561 ggagcgtggc ctgcgccgac gtgaaggccg ttccggccac ggccaccacg ttggtgaagc
     1621 cggcggcgaa gacgcgcagg tggtcgaaat atccctccac caggatggcc tcgtcgaggc
     1681 gacgaattgc atcacgcgcc atggccaggc cgaacagcag ctgacccttc tggtagaccg
     1741 gcgattcggg ggagttgatg tacttggcct cgtcgtcccc cagcgtgcgg ccgccgaagg
     1801 cgatgacacg accgctggac gaggcgatcg gcaccatcag acggttgcgg aagcggtcgt
     1861 atccgccgcc cgcatccttg ggcacgacga ggcccgcccg cgagaggatc tccacggtga
     1921 agcgcttcga ggccacgtcg aagaagttgg cccagccgtc gggcgcgaag ccgaggccga
     1981 acgattcggc ggtttccatc gtgatgtcgc gcgattcgag gtactcccgg gcggggcgcc
     2041 ccacctcgcg gtggttcagc cgctcgcgga agaattcctg cgcgagcgcg agcgcctcgt
     2101 ggatcctccc gatctcgagg tcggccccgg cgtcttccgt ccgcgtgggg agtggaatgc
     2161 cggcccgctc ggccagcgtg cgcacggctt cagggaaggt gagcttggcg tgctcgatca
     2221 ggaaggagat ggcgttgccc cccgcgccgc agccgaagca gtgatagatc tgccggtccg
     2281 ggttcacgtg gaacgagggg gtcttctcgg tgtggaacgg gcagagcccc ttgtacgaac
     2341 ggccggcgcg ggtcagccgc acctgctcgc ccaccagcgc cacgatgtcg gtggcctcgc
     2401 gcacccggtc gatcagatcc tgtggatatc tcacgcgcgc tcccggctca ttcgagatcc
     2461 accaccgtga cgcccgtgcc gccttcccag gtgtccccca ggcgatggct cacgatgtcg
     2521 gtacggtccg agagccactt ccgcacggcc gcgcggagg
//
LOCUS       DASVOD010000543         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_141174, whole genome shotgun sequence.
ACCESSION   DASVOD010000543 DASVOD010000000
VERSION     DASVOD010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141174"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VF720_12035"
     CDS             complement(<1..1028)
                     /locus_tag="VF720_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880134.1"
                     /translation="MKLLLQYLRNYWKLVALALVLAAINQVFSLLDPLIFRHVIDEYV
                     TQTKKYTTEQFFKGVSLLLLAAVSVAFISRVAKNFQDYFINLVTQRLGAQIYADGIRH
                     SLRLPYQVFEDQRSGETLGRLQKVRTDVERLISASINILFTTLVGIVFVMVYAFSVHW
                     IIAPVYLITMPLLGVMSSVLSKKIKTIQKRIVAETSALAGSTTESLRNIEVVKSLGLA
                     GQEIARLNSTTDRILALELEKVRYIRTLSFIQGTAVNALRTSILFLMLYLIFAGQISV
                     GQFFSLFIYSFFIFGPLQELGNIINIYREAEVSLKNFQEILDTAPEPVPARPVPFGPI
                     GKLEFDRVV"
     gene            complement(1042..1905)
                     /locus_tag="VF720_12040"
     CDS             complement(1042..1905)
                     /locus_tag="VF720_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX7880135.1"
                     /translation="MTTDVSAAPRVIRIGHSPDPDDAFMFYALSTGKVVVDGCVIEHR
                     LEDIETLNRRAMNGEYEMTAFSAHAYPEVAKHYQVMMTGASMGRGYGPIVLSQTPMAL
                     GDLAGKKLAIPGVKTTAWLLFQLFGPAVTPVVVPFDTIMEEVEAGRVDAGLVIHEGQL
                     TWTEGSLHKVCDFGTIWEEATGGLPLPLGLDAVRRDLGPELIARLATGLSDSIRYAYE
                     HEADALEYARGFGRGIQNELNRKFVRMYVSDDTIDMGEPGVRALEQLYDRAVAKGLLA
                     ARPPLDLIPVG"
     gene            complement(1902..2609)
                     /gene="rpiA"
                     /locus_tag="VF720_12045"
     CDS             complement(1902..2609)
                     /gene="rpiA"
                     /locus_tag="VF720_12045"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015844279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX7880136.1"
                     /translation="MTTPISANELKRQAALLGIESVKDGMVVGLGTGSTAEWAVRLLG
                     ERVKQGLSIQGVPTSDATAKLAQEVGIPLVDFESIWGIDLTLDGADEVDPDLNLVKGG
                     GGALLREKIVAAASKELIVLVDPSKLVPRLGEHFPLPVEVTPFGWQQTARHLAAVGCQ
                     PKRRTTGSRPCTTDNGNHILDCRFPGGIENPGRTESAIDRIPGVVETGLFVRMTRCVI
                     VGYPDGARAMRKEPTSP"
BASE COUNT          488 a          908 c          946 g          522 t
ORIGIN      
        1 accacgcggt cgaactccag cttcccgatc ggcccgaacg gcacggggcg ggccggcacc
       61 ggttccggcg cggtgtcgag gatctcctgg aagttcttga gggagacttc ggcctcgcgg
      121 tagatgttga tgatgttccc gagttcctgc agcgggccga agatgaagaa cgagtagatg
      181 aagagggaga agaactgccc gaccgagatc tgcccggcaa agatgaggta gagcatcagg
      241 aagaggatgc tcgtgcgcag cgcgttgacg gccgtcccct ggatgaacga cagcgtgcgg
      301 atgtaccgca ccttctcgag ctcgagcgcc aggatccggt ccgtggtgct gttcagccgg
      361 gcgatttcct gccccgccag gccgaggctc ttcaccactt cgatgttccg gagactctcg
      421 gtggtcgagc cggccagcgc cgacgtttcg gccacgatcc gcttctggat ggtcttgatc
      481 ttcttcgaca gcacgctcga catgacgccg aggagcggca tcgtgatgag atagaccggg
      541 gcgatgatcc agtgcacgct gaaggcgtac accatcacga agacgatccc cacgagcgtc
      601 gtgaaaagga tgttgatcga ggccgagatg agccgctcga cgtcggttcg caccttctgc
      661 aggcgcccga gcgtctcgcc gctccgctgg tcctcgaaca cctggtaggg gaggcgcagc
      721 gaatgacgga tgccgtcggc gtagatctgc gcgccgagcc gctgcgtcac caggttgatg
      781 aagtagtcct ggaagttctt ggccacgcgg ctgatgaacg ccacgctgac cgccgccagg
      841 agcagcaggc tcacgccctt gaagaactgt tcggtggtgt attttttcgt ctgcgtcacg
      901 tactcgtcga tgacgtgacg gaagatcaag gggtcgagga gcgaaaagac ctggttgatg
      961 gcggccagca cgagggccag cgccaccagc ttccagtagt ttcggagata ttggaggaga
     1021 agtttcaacg cgtcctttcg atcaacccac cgggatcagg tcgagcggcg gccgggcggc
     1081 caggagtccc ttggccacgg cccggtcata cagctgctcg agcgcgcgaa cgcccggttc
     1141 ccccatgtcg atggtatcgt cgctcacgta catccgcacg aacttgcggt tgagctcgtt
     1201 ctggatgccg cggccgaagc cgcgcgcgta ctccagcgca tcggcctcgt gttcgtaggc
     1261 gtagcgaatg ctgtccgaga gtccggtggc cagccgggcg atcagctcgg ggccgaggtc
     1321 gcggcgcacg gcatccaggc cgagcggcaa cgggagcccg ccggtggcct cctcccagat
     1381 ggtcccgaag tcgcacacct tgtggaggct cccttccgtc caggtgagct gcccctcgtg
     1441 gatcaccagg ccggcatcca cgcgtcccgc ctccacctcc tccatgatgg tgtcgaacgg
     1501 caccacgacg ggcgtgacgg ccgggccgaa gagctgaaag aggagccagg cggtggtctt
     1561 cacgcccgga atggccagct tcttgccggc caggtcgccc agggccatcg gcgtttgcga
     1621 gaggacgatc ggtccgtagc cgcggcccat cgaggcgccg gtcatcatca cctggtaatg
     1681 cttggccact tcgggatagg catgcgccga gaaggcggtc atctcgtatt cgccgttcat
     1741 cgcccggcga ttcagcgtct cgatgtcttc caaccggtgc tcgatgacgc acccgtccac
     1801 caccaccttg ccggtggaga gggcgtagaa catgaaggcg tcgtcgggat cgggggagtg
     1861 gccgatgcgg atcacccgcg gcgcggcgct cacgtcggtc gtcatggcga ggttggttcc
     1921 tttctcatcg cgcgggcgcc gtcgggatag ccgacgatga cgcagcgtgt catgcgaacg
     1981 aagaggccgg tttccaccac gccgggtatc cggtcgatgg ccgattcggt ccggccggga
     2041 ttttcgatgc cgccgggaaa gcgacagtcg aggatatgat tgccgttgtc ggtcgtgcag
     2101 ggcctcgagc cggtcgttcg gcgcttcggc tggcacccga cggccgccag gtgccgggcc
     2161 gtctgctgcc agccgaacgg cgtcacctcc accggcaggg ggaagtgctc gccgaggcgc
     2221 ggcaccagct tgctcgggtc aaccaggacg atcagttcct tcgaggccgc cgccacgatc
     2281 ttctcgcgca ggagcgcccc gccgcccccc ttgaccaggt tcaggtcggg atccacctcg
     2341 tcggccccat ccagggtcaa atcgatgccc cagatggatt cgaaatcgac caatgggatt
     2401 ccgacttcct gagccaattt tgccgtggcg tcgctggtcg gcacgccctg gatcgagagg
     2461 ccttgcttga cccgttcacc gaggaggcgc acggcccact cggcggtgga gccggttccg
     2521 aggcccacga ccatcccatc cttgaccgat tcgatcccca gaagggcggc ctgtcgcttc
     2581 agctcattgg ccgaaatagg agtggtcatt cgggaattgg acctcgccgg ttagggggtc
     2641 ggccatcata ctgttcccgg aaatcgccca caaccgacag ggaggttcga tgccgttccc
     2701 gagtttcgac tacatggaat tcgccaagat ggaccacggc ccgggcgtgc gttttcccct
     2761 cggcttttcc ggcttcaacg tggccgatcc ggatgtcctc ggcgtggatc ccgccgatgt
     2821 ccgcctgatt ggcgctcccg gttacggcga tcccggcgtt cggg
//
LOCUS       DASVOD010000544         2310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_244544, whole genome shotgun sequence.
ACCESSION   DASVOD010000544 DASVOD010000000
VERSION     DASVOD010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244544"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1168
                     /locus_tag="VF720_12050"
     CDS             227..1168
                     /locus_tag="VF720_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880137.1"
                     /translation="MPRRDRAHLASLILALSLGLVAVPHTVTASGGIGDMYISSDASD
                     LVRAYGGNTGTFLGVHASGVLADGHLGLHFGDTNNRLLVGSWSGGVDEFNATTGAYIK
                     TYNTAGNWQWAGLYAPNGNVLITSTMTNEVLEFDPTTGAFIQQFCPVYFPSDMRIGPN
                     GNLFVCSFGGGFVEEHDINTGALLTTIPLPAQAQANDVAWLPNGDMLVTAMRTNLVYR
                     FDSLYTLVGSFAGTGWGNPHGIDIHPTNGRIYVVDGITTQVHVFDPVTFAELDPAHLT
                     PGPGDKIVDIEFRRYDEPLNVQPSTWSRIKALGNENP"
     gene            complement(1244..1984)
                     /locus_tag="VF720_12055"
     CDS             complement(1244..1984)
                     /locus_tag="VF720_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880138.1"
                     /translation="MKAVILGGTSGMGRSLAQQLAGRGDLVFLLGRDAAALEQGVTDL
                     RMRHPERQAAGSAVCDLESPEGFQGALDAADRALNGFDTVIVTAGLFATQDELEADIE
                     KTRRLLTVDFANTVVFCERVRAMLLPRGGGRLVVFSSVAGDRGRKPVALYGAAKAGLS
                     TYLEAMDHRYHAAGLHVLCVKPGFVKTAMTAGLKPPPFAGEPDRVAREVIRAMDKGLP
                     LVYSPPIWGLVMLVIRFLPRFVMRRIRF"
     gene            complement(1984..>2310)
                     /locus_tag="VF720_12060"
     CDS             complement(1984..>2310)
                     /locus_tag="VF720_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880139.1"
                     /translation="TRLGAASPLCVIKDCGPEGRGTLSFPLEGTSIAVDMPMGPQIQR
                     IVDRLNEFVVAAGGRIYLTKDRFTTAAHFRAMEPRLPAFEALRDKWDPERRLRSAQSR
                     RLFGDH"
BASE COUNT          414 a          793 c          730 g          373 t
ORIGIN      
        1 caatgccagg acaattccga tcgatccgat ggccgttcgc cgtgctgtca tgtgggagcc
       61 tcctggaacg agttgcggtg gggcgggggg ctgggcggga cagtctcgga ttatcgtgta
      121 ccgacatgag ggaaagccag cattttcgcg ggtttccggg ccatttggag gtaaactggg
      181 cctcacttcc cggcaccaca cccccacccc ggaccggagg acaggcatgc cccgtcgtga
      241 ccgcgctcat cttgcctcac tcatcctcgc cctttccctc ggcctggttg ccgtcccaca
      301 caccgtaacc gcctccggcg gcatcggcga catgtacatc tcgagcgatg cttcggacct
      361 cgtgcgcgcg tacggcggca acacagggac gttcctcggc gttcatgcca gcggcgtgct
      421 ggccgacggg cacctggggc tccatttcgg ggatacgaac aatcgcctgc tggtgggcag
      481 ctggagcggc ggcgtggatg agttcaacgc caccaccggc gcgtacatca agacctacaa
      541 caccgccggc aactggcagt gggccgggct ctacgcgccg aacggcaacg tgctgatcac
      601 ctcgacgatg accaacgagg tcctggagtt cgacccgaca accggggcct tcatccagca
      661 gttctgcccc gtttacttcc cgtcggacat gcgcatcggc cccaacggca acctgttcgt
      721 ctgctcgttc gggggcggtt tcgtggagga acacgacatc aacaccggcg cgcttctcac
      781 caccatcccc ctccccgcgc aggcccaggc caacgacgtt gcctggctcc ccaacggcga
      841 catgctggtg acggccatgc gcacgaacct cgtgtatcgc ttcgattcgc tctacacgct
      901 ggtcggctcg ttcgcgggaa ccggctgggg aaatccgcat ggcatcgaca tccatccgac
      961 gaacggacgg atctacgtgg tggacggcat cacgacgcag gtccacgtgt tcgatcccgt
     1021 cacgttcgcg gaactcgacc cggcgcacct gacgccgggt ccgggcgaca agatcgtcga
     1081 catcgaattc cggcgatacg acgagccgct caacgtgcag ccgtcgacct ggagccggat
     1141 caaggcgctg gggaacgaga acccctgaga gcacccgcag caccacgtcc gtgccggttc
     1201 cggtggaatc gacgacgtgg gggaagatga agagcctgta ccgctaaaac ctgatgcggc
     1261 gcatcacaaa gcgcgggagg aagcggatca cgagcatcac gaggccccag atcggcggcg
     1321 aatagacaag tggaagtccc ttgtccatcg cccggatgac ctcgcgcgcc acgcggtccg
     1381 gttcccccgc gaacggcggc ggcttcaggc ccgcggtcat ggccgtcttc acgaaccccg
     1441 gcttcacgca aagcacgtgg agtcccgcgg cgtgataccg atggtccatg gcttccagat
     1501 aggtggagag gccggctttg gcggctccgt acagtgccac cggctttcgg ccgcgatcgc
     1561 cggccaccga tgaaaagacg acgagccgtc cgccgccacg tggcagcagc atggcgcgca
     1621 cccgttcgca gaagacgacg gtgttggcga agtccaccgt caggaggcgg cgtgtcttct
     1681 cgatgtcggc ttcgagctcg tcctgggtgg cgaagagtcc ggccgtcacg atgacggtgt
     1741 caaaaccatt caaggccctg tccgcggcgt cgagcgcccc ctgaaagcct tcgggagatt
     1801 cgagatcgca caccgcggac cccgccgcct gccgctccgg gtggcgcatc cggagatcgg
     1861 tcaccccctg ctcgagcgcc gcggcgtcac ggccgagcag gaacaccaaa tcgccgcgac
     1921 ccgccagctg ctgggcgagc gaacgcccca tgcccgaggt gcctcccagg atcactgcct
     1981 tcatcaatga tctccgaaca ggcgccgcga ttgcgcgctc cgcaatcgcc gctccggatc
     2041 ccacttgtcg cgaagcgcct cgaaagcggg aaggcgcggc tccatcgcgc ggaagtgcgc
     2101 cgcagtcgtg aagcgatcct tggtgaggta gatgcgcccg ccggcggcca ccacgaattc
     2161 gttgaggcga tccacgatcc gctggatctg cggccccatc ggcatgtcca ccgcgatcga
     2221 tgtcccttcg agcgggaacg acagcgttcc gcgtccctcc ggtccgcagt ccttgatgac
     2281 gcagagcggg gaggccgcgc cgagccgggt
//
LOCUS       DASVOD010000545         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_37954, whole genome shotgun sequence.
ACCESSION   DASVOD010000545 DASVOD010000000
VERSION     DASVOD010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37954"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF720_12065"
     CDS             complement(<1..353)
                     /locus_tag="VF720_12065"
                     /inference="COORDINATES: protein motif:HMM:NF025104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX7880140.1"
                     /translation="MTRTNVTRFLIIGAGDAGRMAAEEMLRHADSGLVPVGFIDDDPA
                     KIGTTVAGLPVAGDRSRIHDAIATLSADEILIALPSASGAAVRGIIRWCESERVRFRV
                     VPGIWEIIRGDVHLT"
     gene            complement(406..2589)
                     /locus_tag="VF720_12070"
     CDS             complement(406..2589)
                     /locus_tag="VF720_12070"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX7880141.1"
                     /translation="MNPAILRRTARSACRFLLLLLVFMAGVSQPARAQQWTAYANPND
                     IKDITQINGDLWMATTGGALYYDFDANLFHQVTRRAAGGPLSQLLTSVAYDSVSGLMF
                     FGSEDLGVSQYDPVFERWERFEFVPDNRINNVSALDGEVQIGTAAGFAIRRTATRTDI
                     CNDIDRGCCGSVPGSCDFPNFDVREWASPADGRLWAATAGGPAEFDGVKWTTRAIPGL
                     TDIHSVEAINGVVYAAGAGSRQVYRWNGETQAWQAAAEGLRDQGLGDAVRLVNTGGSL
                     YLCANYGLFRRDGASWVSTGLEDHTVRSVVAVDILQADLAAATRDGLFVRRLVGPGTQ
                     WEQRLAPGPPQRADGQAVAGAPDGTLWIGTLGGVQGLTTEGEWLDFRNGASGGLAPFD
                     LFSIYAARDNKLWVGKCCCHSVPNCPTQFIEADDDISPVLSAWDGWGMAEDSQGRLWI
                     GSNSTGLTVLGADGTHITDIPPASGGLASPSVRALATRNNDVWIGHEQRGLQILRTGG
                     NPANPAGFMWKTFTPAQLPDNAIAAIEIRDRDTYVLTSSYLVQYTDEVRVRQWPLNFD
                     GEPRRGTGLAVDRKGNRWIGTTAGVVVINTAGESSLLTTRNSDLIADDVIDVDLDPRT
                     GDILFATRIGTNRLVPGAGPKPGGDAERYLFPNPFHADGSVRVKIGGGSADNAEVFDL
                     AGRSVARFDPASGWDGSGVDGATVAPGIYLVVLDGAEPLRLAVLR"
     gene            complement(2657..>4645)
                     /locus_tag="VF720_12075"
     CDS             complement(2657..>4645)
                     /locus_tag="VF720_12075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7880142.1"
                     /translation="RTPEDTLQLIQSLKAHPHAGHPLDIILVDNASGDDTAARVRAAH
                     PDVRIIESPVNRGFAAGVNLGLAAAQGKYLLILNPDIVVHPGSLEALVAFLDAHPQAG
                     LVAARLLNPDGTLQPTCRTRYTLKTILLRRTPLGKLFPNDPTVREHLMLDYDHATPRN
                     VDWVAGACMMVRREALDEVGPMDERYFLYFEDVDWCTRMHRRGWGVWYVPDAVMTHGY
                     RRASAGGFNKATRAHAESLLRFWEKWSAMLYLARRYRGAVRNAVFLAVDLLAILVAFG
                     LAYQLRNWLAPVMDKPTFPVSHYQTFLVTTLVTAIVAFALGGLYREEERGDWVDTLFA
                     TGKALLGASLFLLLTTFLLYPPDSRPYSRFIIGSFWPLAMLLVTLERRLLYGLLERAR
                     AGRLNVKRVALVGAGAWMDPLARTLRTDPRHGFEPIRVTLPPADRPEALRATLEDERV
                     SDVVLSTEVLPTGPGEARALLEPLRQAGIRIHLSGPLSDLIGKDARVTPLAGTTLLSL
                     DQGGAGTGSRVARRLFESLVALLFFVLSLPAALVAAVLVPLTGSRGRLVDGGARWEVE
                     GPMSGPVRRLSLDRFPGWWAVLLGRRCLVGGDAARPGVFDAPDMALPAESGYSLPWSP
                     ERDLKTIVRDLLARLKTGNDTRSGPPPVLERSPRSALR"
BASE COUNT          750 a         1600 c         1641 g          654 t
ORIGIN      
        1 tgcgtcaggt gcacatcgcc ccggatgatc tcccagatgc cgggcaccac gcggaagcgc
       61 acccgctcgc tctcgcacca gcggatgatg ccgcggaccg ccgcgcccga ggcggaaggg
      121 agcgcgatca ggatctcgtc cgcggaaagg gtggcgatgg cgtcatggat ccggctccga
      181 tcgcccgcca ccggcaggcc ggccacggtg gtgccgatct tggccgggtc gtcgtcgatg
      241 aagccgaccg gcacgaggcc cgaatcggcg tgacggagca tttcttcggc ggccatccgc
      301 ccggcgtcgc cggcgccgat gatcaggaat cgggtgacgt tcgttcgcgt catgccgccg
      361 ctcggatccc cggagtgatg gaagaagtgc gccgagccgg tcggatcagc gcagcaccgc
      421 caggcgcagg ggctcggcgc cgtccagaac gacgaggtag atgccgggcg ccacggtggc
      481 gccgtccacg ccggaaccgt cccatcccga ggcgggatcg aagcgcgcga cggaccgccc
      541 ggccaggtcg aatacctcgg cgttatcggc ggaaccgccc ccgatcttga cccggaccga
      601 cccgtcggca tggaacgggt tcgggaagag gtagcgttcc gcgtcgccgc cgggtttggg
      661 gcccgcacca ggcaccagcc ggttggtgcc gatccgcgtg gcgaagagga tgtcccccgt
      721 tctgggatcg aggtcgacat cgataacgtc gtcggcgatc aggtccgagt tgcgcgtggt
      781 gaggagcgac gactcgcccg ccgtgttgat gaccaccacg ccggccgtcg tgccgatcca
      841 gcggttcccc ttccggtcga cggccaggcc cgtgccccgg cgcggctcac cgtcgaaatt
      901 gaggggccac tggcgcacgc gcacctcgtc cgtgtactgg acgagatacg acgaggtgag
      961 cacgtacgtg tctcggtcgc ggatttcgat ggccgcgatg gcgttgtcgg gaagctgcgc
     1021 cggcgtgaac gtcttccaca tgaagccggc cgggttggcc gggttgccgc cggtgcgaag
     1081 gatctgcagg ccgcgctgct catggccgat ccacacatcg ttgtttcgcg tggccagcgc
     1141 ccggacggac ggcgaggcca ggccccccga cgcgggggga atgtcggtga tgtgggtgcc
     1201 gtccgcgccg agcacggtga ggcccgtcga gttgctgccg atccagaggc gcccctggga
     1261 gtcctcggcc atcccccagc cgtcccaggc cgagaggacc ggcgaaatgt catcatctgc
     1321 ctcgatgaac tgggtcgggc agttcggcac gctgtgacag cagcacttcc ccacccacag
     1381 cttgttgtcg cgggccgcgt agatgctgaa gagatcgaac ggcgccagcc ccccggacgc
     1441 gccgttgcgg aagtcgagcc actccccctc ggtcgtgaga ccctgcacgc cgccgagcgt
     1501 gccgatccag agcgtgccgt cgggcgcacc cgccacggcc tgcccatcgg cccgctgagg
     1561 cggtccgggc gccaggcgct gctcccactg cgtgccgggg ccaaccagcc gacgcacgaa
     1621 gagcccgtcg cgcgtggcgg ccgccaggtc ggcctggagg atgtcgactg caaccaccga
     1681 gcgcaccgtg tggtcttcga ggccggtgga cacccagctt gccccgtcgc ggcggaagag
     1741 gccgtagttg gcgcacagat aaagcgagcc gccggtgttc accagcctca ccgcgtcgcc
     1801 gagcccctga tcgcgcagcc cttcggcggc ggcctgccag gcctgggttt ccccgttcca
     1861 ccggtacacc tggcgcgagc cggcccccgc cgcgtagacg acgccgttta tcgcctcgac
     1921 gctgtggatg tccgtgagtc ccgggatggc acgcgtcgtc cacttgacgc cgtcgaactc
     1981 ggccgggccc ccggcggtgg cggcccacag gcgtccgtcg gccggcgagg cccactcgcg
     2041 cacgtcgaaa ttcgggaagt cgcagctgcc cggcaccgag ccgcagcatc cgcggtcgat
     2101 atcgttgcag atgtcggtgc gggtggccgt gcggcggatg gcgaagccgg ccgccgtgcc
     2161 gatctgcact tcgccgtcca gcgccgacac gttgttgatc cgattgtcgg ggacgaattc
     2221 gaaacgctcc caccgctcga aaacggggtc gtactggctg acgcccaggt cctcgctgcc
     2281 gaagaacatg agccccgaca ccgagtcgta ggcgaccgac gtgaggagtt gcgacagcgg
     2341 cccccccgcg gcgcgccggg tcacctggtg aaagaggttg gcgtcgaagt cgtaatagag
     2401 ggcgccgccg gtcgtggcca tccagagatc gccgttgatc tgggtgatgt ccttgatgtc
     2461 gttgggattg gcgtaggccg tccactgctg ggcgcgggcg ggttgagaca cgccggccat
     2521 gaaaacgagt agcagcagca ggaaacggca ggcggaacgg gcagtgcggc gcaggatcgc
     2581 cggattcatc tctcgggggc ctcctcgact gacctgggac tcggggtcca tcacagggga
     2641 aggacgacgg attccattag cgaagggccg atcgaggact cctctcgagg actgggggcg
     2701 gtccgctccg cgtatcgttg ccggttttca ggcgggccag caggtcgcgg acgatcgtct
     2761 tcagatcccg ctccggcgac cacggcaaag aataaccgct ttcggccggc aaggccatgt
     2821 ccggcgcgtc gaagaccccc ggacgggcgg catcgccacc cacgaggcac cggcgcccca
     2881 ggagaaccgc ccaccacccg ggaaaccgat cgagcgacag ccggcgcacc gggccggaca
     2941 tcggcccctc cacttcccac cgggcgccgc catccaccag ccgcccgcga ctgccggtca
     3001 gcggcaccag tacggccgcc acgagcgccg cggggagcga caggacgaag aagaggagcg
     3061 ccaccagcga ttcgaacagg cgccgggcca cccggctccc cgtcccggcc cccccctggt
     3121 cgagcgagag gagcgtcgtg ccggccagcg gcgtcacccg tgcatccttg ccgatcaggt
     3181 cggacagcgg ccccgacagg tggatgcgga tgccggcctg ccgaagcggt tcgaggagtg
     3241 cccgagcctc gcccggtccc gtggggagga cctcggtgga aaggaccacg tcgctcacgc
     3301 gctcatcctc gagcgtggcc cgcagcgcct cggggcggtc ggccggcggg agcgtcaccc
     3361 ggatcggctc gaagccgtgg cgcgggtcgg tgcgcagcgt gcgcgcgagc gggtccatcc
     3421 aggctcccgc gcccaccagg gccacgcgct tcacattgag gcgtcccgcg cgggcccgtt
     3481 cgagcagccc gtacaggagc cgccgctcga gcgtgacgag gagcatcgcc aaaggccaga
     3541 acgacccgat gatgaagcgc gagtagggac gactgtcggg cggatagagc aggaacgtcg
     3601 tcagcagcag gaacaggctc gcgccaagca gcgccttgcc ggtggcgaag agcgtgtcga
     3661 cccagtcgcc gcgctcctcc tcgcgataga ggccgcccag cgcgaacgcc acgatggcgg
     3721 tgaccagcgt cgtcacgaga aaggtctggt agtgcgagac cgggaacgtg ggcttgtcca
     3781 tgacgggagc cagccagttc cggagctgat acgcgaggcc gaacgccacg aggatggcca
     3841 ggaggtcgac tgccaggaag accgcgttcc gcacggcccc ccgatatcgc cgcgccaggt
     3901 aaagcatggc gctccacttt tcccagaaac gaaggaggct ctcggcatgg gcccgcgtgg
     3961 ccttgttgaa gccgcccgcc gaggcccgac ggtagccgtg cgtcatgacg gcatcgggca
     4021 cgtaccacac gccccagccg cgccggtgca tgcgcgtgca ccagtccacg tcctcgaaat
     4081 agaggaagta gcgctcatcc atcggcccca cttcatcgag cgcctcgcgc cgcaccatca
     4141 tgcaggcgcc ggccacccaa tccacgttac gcggcgtggc atggtcgtaa tcgagcatca
     4201 ggtgctcgcg cacggtcgga tcgttcggga agagcttccc gagcggcgtg cggcgcagga
     4261 ggatggtctt gagcgtgtac cgggtccggc aggtcggctg cagcgtcccg tccgggttga
     4321 ggagccgcgc cgccacgagg ccggcctggg gatgcgcgtc gaggaacgcc accagcgcct
     4381 ccaggcttcc cggatggacc acgatgtccg ggttcaggat gaggaggtat ttcccctggg
     4441 cagcggcgag ccccaggttc acgcccgcgg caaacccccg gttgaccggg ctctcgatga
     4501 tccggacgtc cggatgggcc gcccggaccc gggccgccgt gtcatccccc gaagcgttgt
     4561 cgaccaggat gatgtcgagc gggtgcccgg catggggatg cgccttcagg ctctggatga
     4621 gctgaagcgt atcctcgggg gtccg
//
LOCUS       DASVOD010000546         2779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_608819, whole genome shotgun sequence.
ACCESSION   DASVOD010000546 DASVOD010000000
VERSION     DASVOD010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608819"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VF720_12080"
     CDS             <1..527
                     /locus_tag="VF720_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3641 domain-containing protein"
                     /protein_id="HEX7880143.1"
                     /translation="GVFGRSIEALRTLNGLGYGVPGSGLTLNLVYNPVGAFLPAAQNS
                     MEADFHRQLERKHGIVFNSLYAITNMPISRYLEFLIDSGNYDAYMQRLVAAFNPAAVP
                     GVMCRTMISIGWEGTLYDCDFNQMLERPVGFGAPAHIADFDPSRLTARKIVTGRHCYG
                     CTAGAGSSCGGNTA"
     gene            482..2122
                     /locus_tag="VF720_12085"
     CDS             482..2122
                     /locus_tag="VF720_12085"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014438118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX7880144.1"
                     /translation="MHGGGGIELRGEHRVSTPTFEQFGSRLVTAGATGFCLDWSRIPG
                     LPEVVPALRPGLEAALASMRDLEAGAIANPDEDRMVGHYWLRAPERAPSPELTAAITT
                     TIDRVHRFASDIHSGAIAPPEAPAWRNVLLIGIGGSALGPQLVHDALGTGRDRLRLFF
                     CDNTDPDGMDRLFESIAAAEGGLAATLVVVISKSGGTKETRNGQVEAAHAWRRAGLDM
                     TRQAVAVTGEGSALDKQARAEKWLAIFPMWDWVGGRTSVLSAVGLLPAALQGLPIDRL
                     LEGARLMDEATRVADPLANPAALLAAAWHHEGNGRGERDMVILPYKDRLLLMSRYLQQ
                     LVMESIGKEKDLEGRVVHQGLAVYGNKGSTDQHAYVQQLRDGLNNFFVTFIQVDLDRH
                     GDAVEVEPDTTSGDFLLGFFLGTRRALYDSGRGSLTIRMPDVSAFSLGALIALFERAV
                     GLYAFLIGINAYHQPGVEAGKKAAGEVIELQRQLLAQLRNVSAPASAAELASAAGSVD
                     VETAWHILEHLAANSGRGVRRHGEGTGARYSMVRGAIG"
     gene            complement(2092..>2779)
                     /locus_tag="VF720_12090"
     CDS             complement(2092..>2779)
                     /locus_tag="VF720_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880145.1"
                     /translation="PSWMRLAPLVLVWLAACGHDVTPTEPYDTPEEIPVPTMLLVRPA
                     VFGPYLTWDTPDSDFVIVAGWHVYRRRPDGDERRLTSEPLVMRQYQDPDTPQVGQTFY
                     WVTAESRGGVESLPTPPVRFLRDDLPPMPPTGLLADAGPDLVLLRWQTGLEFDLAGYR
                     VERDAVEIGIVGDPAMPAFIDRTVVAGATYVYAIRAVDFSNLVSEPGPGVTVTIPLTN
                     GQPIAPRTIE"
BASE COUNT          457 a          900 c          972 g          450 t
ORIGIN      
        1 acggggtttt cggacgatcg atcgaggcgc tgcggacgct gaacggcctt ggctacggcg
       61 tgcccggcag cggcctcacg ctgaacctcg tttacaaccc ggtgggcgct ttcctgccgg
      121 cggcgcagaa ctcgatggaa gccgatttcc accgccagct cgagaggaag cacggcatcg
      181 tcttcaacag cctctatgcc atcaccaaca tgccgatcag ccgctacctc gaattcctga
      241 tcgactccgg caactacgac gcctatatgc agcgcctggt tgccgccttc aatcccgccg
      301 ccgtccccgg cgtcatgtgc cgcaccatga tctccatagg ctgggagggc acactctacg
      361 actgcgactt caaccagatg ctcgagcgcc cggtcggatt cggggcgccg gcgcatatcg
      421 ccgacttcga tccatcgcga ttgaccgctc ggaagattgt cacggggcgg cattgctacg
      481 ggtgcacggc gggggcggga tcgagctgcg gggggaacac cgcgtgagca ctcccacgtt
      541 cgagcagttc ggaagccgac tggtcaccgc gggagccacc ggcttctgcc tcgactggag
      601 ccgcattccc ggccttcccg aagtggtccc ggccctccgt ccggggctcg aagccgcgct
      661 ggcctcgatg cgagatctcg aagccggcgc catcgccaat ccggacgaag accggatggt
      721 ggggcactac tggctgcggg cgccggagcg ggcgccgtcg ccggagctca ccgccgcaat
      781 cacgacgacc atcgatcgcg tccaccggtt cgcgtccgac attcattcgg gggccatcgc
      841 gccaccggag gcgccggcct ggcgcaacgt cctcctgatc ggcatcggcg gctccgcgct
      901 cgggccacag ttggtgcatg acgcgctcgg cacggggcgc gatcgacttc gcctcttctt
      961 ctgcgacaac accgaccccg acggaatgga tcggctcttc gagtcgatcg cggcggccga
     1021 aggaggattg gcagcgactc tggtcgtcgt catctccaag tcgggcggca cgaaggaaac
     1081 gcggaacggg caggtcgagg cggcgcacgc gtggcggcgg gccggcctcg acatgacgcg
     1141 ccaggccgtt gcggtgacgg gcgaggggag tgcgctcgac aagcaggccc gcgcggaaaa
     1201 gtggctggca atcttcccga tgtgggattg ggtcggcggg aggacctcgg tgctgtcggc
     1261 ggtcggcttg ttgcccgcgg cactgcaggg tctgccgatc gatcgactgc tggaaggggc
     1321 gcgcctcatg gatgaagcca cgcgcgttgc cgacccactc gccaatccgg ccgcgctgct
     1381 ggccgcggcg tggcaccatg aaggaaacgg gcggggcgag cgcgacatgg tcatccttcc
     1441 ctacaaggat cgcctcctcc tcatgagccg gtatctccag caattggtca tggaatcgat
     1501 cggcaaggaa aaggacctcg aggggcgcgt cgtgcaccag gggctggcgg tgtacggcaa
     1561 caaggggtcc accgaccagc atgcgtacgt gcagcagttg cgcgatggcc tgaacaactt
     1621 cttcgtcacc ttcatccagg tggatctcga ccgccatggt gatgcggtgg aggtcgagcc
     1681 cgacacgacg tcgggtgact tccttctcgg atttttcctc ggcacgcggc gcgcgcttta
     1741 cgacagcggt cgcggatcgc tcacgatccg gatgccggac gtgtcggcgt tctcgctcgg
     1801 ggcgttgatc gcgttgttcg agcgggcggt gggactttat gccttcctga tcggcatcaa
     1861 tgcgtaccac cagccgggcg tggaagccgg gaagaaggcg gcgggcgagg tgatcgaact
     1921 gcagcggcag ttgctggccc aactgcgtaa tgtgtcggcg cccgcctcgg cggccgagct
     1981 cgcatcagcc gcggggtccg tggatgtcga gacggcgtgg cacatcctcg agcatctcgc
     2041 ggccaattcc gggcggggcg tgcgccggca tggcgagggg accggggcgc gctactcgat
     2101 ggtgcgtggt gcgatcggct gaccgttcgt caatgggatc gtgaccgtta cgcccggccc
     2161 cggctcgctg accaggttcg aaaaatcaac cgcacggatg gcatacacgt aggtggcgcc
     2221 cgccacgacc gtgcgatcga tgaaggccgg catcgccggg tcgcccacga tgccgatttc
     2281 cacggcgtcc cgctccacgc gataacccgc caggtcgaac tccaggccgg tctgccagcg
     2341 gagaaggacg agatcggggc cggcatcggc cagaaggccg gtgggcggca tcggcgggag
     2401 gtcgtcccgc aggaagcgga ccggaggcgt tggaaggctt tccacgccgc cgcggctctc
     2461 ggcggtcacc cagtagaagg tctgccccac ctgcggtgtg tcgggatcct ggtactggcg
     2521 catgacgaga ggctccgaag tgagtcggcg ctcgtcgcca tcggggcgac gccgatacac
     2581 gtgccagccc gccacgatca cgaaatcgct gtcgggcgta tcccaggtga ggtacgggcc
     2641 gaagacggcg ggacgcacga ggagcatcgt cggcaccggg atctcttcgg gcgtgtcgta
     2701 tggctcggtg ggcgtcacgt cgtggccgca ggccgcgagc cacacgagga cgagcggcgc
     2761 gagccgcatc catgacggc
//
LOCUS       DASVOD010000547         9922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_244656, whole genome shotgun sequence.
ACCESSION   DASVOD010000547 DASVOD010000000
VERSION     DASVOD010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9922
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244656"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VF720_12095"
     CDS             <1..441
                     /locus_tag="VF720_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX7880146.1"
                     /translation="MTRTFCVGAAPPRLREVYSQVKEVLMSTLAELAVGTRCINYQIK
                     ACERFEAMGYATIRQDSRVESGYVHGLGHGLGLDIHESPRLGGALSNPDVLAPGHVIT
                     VEPGLYLPDEGVAVRLEDVVYVRPDGRIENLTTYPYDMEIFPEG"
     gene            380..1630
                     /gene="hflX"
                     /locus_tag="VF720_12100"
     CDS             380..1630
                     /gene="hflX"
                     /locus_tag="VF720_12100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX7880147.1"
                     /translation="MVASRISPPIRTTWRSSLRDSFDVPEAASTRRRFHDLEAERARR
                     EKAVLIGVVPRKTQEDALAELALLAETAGAEVVKVVRQRRDVISPGTFVSKGKLEDLK
                     AMANSGEADLFIFDDDLHPNQVKHLQDALQKKILDRSELILDIFAARARTREAKLQVE
                     LAQLEYLLPRLTRMWSHLSRTGGGIGTRGPGETQLETDRRLVREKIGVLKEKMRGVEK
                     ERQVQRNRRRKLHRTALVGYTNAGKSTLMNALTRADVLAEDKLFATLDATTRRMGWAD
                     GTVTLLSDTVGFVRKLPHHLVASFKSTFEEVVEADLLLHVVDASHPDAAPQIDAVERV
                     LEEIGAGSRRTLLILNKADSITDETELAGLALRHPGSVIVSALRASDREELRRRIHEA
                     VLETSRRPAAAAGGNQSGVPKASA"
     gene            complement(1600..2223)
                     /locus_tag="VF720_12105"
     CDS             complement(1600..2223)
                     /locus_tag="VF720_12105"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011714975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HEX7880148.1"
                     /translation="MTMDPSFRRHRPHPWHGLEPGAEAPDVVTAYIEITPFDLVKYEV
                     DKATGYLKVDRPQRGSSSPPTLYGFIPRTYCAARVAALAPGSTAGDGDPLDICVISER
                     PIARSEILLRARVVGGLQMIDASQADDKIVAVLTGDYVWGNVAEMTDLPAALVERLEH
                     YFLTYKLRPGREGDQPVRIARRYDAAHAREVIRASMADYAEAFGTPD"
     gene            complement(2300..2929)
                     /locus_tag="VF720_12110"
     CDS             complement(2300..2929)
                     /locus_tag="VF720_12110"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX7880149.1"
                     /translation="MLQSVLEEAGQHRRLIAAWLMTAAIAFTWAGCSQAPHHSNYPDY
                     RSEHMALHTDGKAPTSDSRHANANDQAINTGRMSYDNQQAVAGAYAVGDTDPNRLRGT
                     TWEWIHLSRGSENVSVIDPQNYTVSFDGDGYVHVRSDCNKAAGPSKLGDGSISINLMR
                     MTDMPCNANSLSDRFTAALTRVSAWRVSGDQLMLEIPGESSLLRFKRAG"
     gene            complement(3099..3920)
                     /locus_tag="VF720_12115"
     CDS             complement(3099..3920)
                     /locus_tag="VF720_12115"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX7880150.1"
                     /translation="MPRLFNRPSLRAIVAVLCLASLPMGSTGCNQKQGTSAETTTGGH
                     ALAGSWQATLPAASSPGRTITLMLNPDMTARMTTEYQNGEPPIVAMGTWRPNGREMAE
                     VTLKREGHNAAPTTMSFRADANSLTAVDFDKDMWGSAGLKLTRTGGPGGATVGGDRNP
                     AGLAGSGWEWTNFSSPTENLSVPDPVKYTLEFAEDGNVAILAACNRGGGPVKLGDGTI
                     SINLLRLTRMACPPGSLDARYTSLLTRVATWSLVNGQLVMEIPAESSVLRFRRVK"
     gene            complement(4040..5722)
                     /gene="ggt"
                     /locus_tag="VF720_12120"
     CDS             complement(4040..5722)
                     /gene="ggt"
                     /locus_tag="VF720_12120"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020470695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX7880151.1"
                     /translation="MTSSASGGEIQPIGRGDASRSPVVAAHGMVAAAQPLAVQVGVDV
                     LKKGGTAVDAAIAVNACLALMEPTSCGLGGDLFAIVWDPTEGKLVGLNGSGRAPMALT
                     IEKVPPDSDGTIPLFSPYSWSVPGCVDGWFALHERYGKLPMSELLGPAIRYANEGFPL
                     SPVIAANWAAGLPRFRDKPGYAEVFMPGGEAPSAGQIFRNPALARTLDAIAAGGREAF
                     YAGPITDAIVRYSQANGGFLARVDFEKHTSTWDAPVHASYRGYDVWELPPPGQGIAAL
                     QMLNMLETFDVAGMGRESADFWHLLTEAKKVAFADRARYYADPAFAAVPVAGLIDKEY
                     ARERAPHIDMTKAAATDAPGEPGGAAPAALNRRETTFLCAADADGMMISLIQSNYTGF
                     GSGYVIPELGFGLQNRGALFSLDPGHPNSLKPGKRPFHTIIPAFLTKDGTPLMAFGLM
                     GGDMQPQGHAQVVINLVDFGMNLQEAGDAIRFHHTDSSEPTGTVMANGGILHIEPGLP
                     EAVLAELRRRGHVIKDEAVANYGGYQAIWRDPVTGTYVGATEKRKDGCALGW"
     gene            complement(5763..6425)
                     /locus_tag="VF720_12125"
     CDS             complement(5763..6425)
                     /locus_tag="VF720_12125"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX7880152.1"
                     /translation="MVNVTIDPSLAGILQVGLVEMTGLTVHTENPRWDVITALCAELR
                     DEFGGAPIGSVPGVEWARKLYSSVGIDPTKHRPSSEALLRRALKGEPLHPVNTLVDAL
                     NWCSLEYLVPMGLYSVPRIEGGVVLRQGKAGEAYEGIGRGIINLEGRLVAADHHGAFG
                     SPTSDSYRTAVSTETTHALALLYVPVGFPEDELAEAVETFAERAQGWCGGQVERTDVL
                     TA"
     gene            complement(6448..8142)
                     /locus_tag="VF720_12130"
     CDS             complement(6448..8142)
                     /locus_tag="VF720_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880153.1"
                     /translation="MKTVKPASPDRPHDGDTRGALPIALALTGLTILPAILHPWIRTR
                     ADSWFHAAIVAEIERAGVPPQDPYFAGLPLQYMWFFHAMLAAVRKVAPVDTFTLMVLV
                     NALALMALILVSAHLAEWIARRTGDEPARAARTAAYVVPLGLGVLVWLVMPIRALKAL
                     AGKSGGMEQLAEAFRWRPLDIGTIRTFLSDFGSVPFFLNKFLVGTAYGLALVAFLVYL
                     AAVLSFIERPRLKPLLVVMPALFLALMFHPVVGFTTVAVSGITGVVLLVLGPRRGGQS
                     FGAVAAWGGSVVVSLLLAVPYLVGVTRGKPGGQLVPIHFDWVNVAGLLVGVLFVAIAA
                     AGLARRLWREGTPWGRMLVTWIIATLVFASFIRLPGPNTTDKFTYLLYLPPAILAGVA
                     LSSRWRGMAGLLAALAILAPANLLAWASYLGEPDYSRRPVEVVEAYDWLRANTPRDAI
                     VLDSRERCDVLVSVPRRQFWGREAYAEQWGYDEGEMARRRYLRDQLVGPSGIAARNGP
                     AIDTGPLAATGAPVFVIVRAEDVGAAAFEAWGEAHPQFSEAHRSAGIRLFQLLRLE"
     gene            8164..9378
                     /locus_tag="VF720_12135"
     CDS             8164..9378
                     /locus_tag="VF720_12135"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX7880154.1"
                     /translation="MANFDLMRRLHTTDNPARIILLVMDGLGGLPREPGGKTELETAR
                     TPNLDALCARGSCGLSYPVAPGITPGSGPAHLALFGYDPITFDIGRGVLEALGIEFPM
                     TPRDLAARGNFCSLDAAGNVTDRRAGRIDTEICVRLCSELSARVRVPGVEVFVEPVKE
                     HRFVVVLRGDGLEEGLTETDPLVTGKPPLAIEPESPAAAATATLFNTFVREAGRVLAE
                     HHPANGVLLRGFAKHPTLPTMSELYGLNCGAVAVYPMYRGLARLVGMSKVTFEGESPA
                     DEFRAVAANWEKHDFFFIHVKKTDSYGEDGNFDAKVGVIESVDQALPILLEKKPEVII
                     VTGDHSTPATMKTHSWHGVPTIIASPWARFDGTTSYGESACARGGLGHFPAVHLMPLA
                     LAHARRLNKYGA"
     gene            complement(9359..9679)
                     /locus_tag="VF720_12140"
     CDS             complement(9359..9679)
                     /locus_tag="VF720_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880155.1"
                     /translation="MNRLESLQQMVAEDPKDGLARFMLGKELLSADLPNEAAEQLAEA
                     IRLAPDHTASYRELGNALASAGRTSEAEVAYTTGLNVADTTGDLQTAREIKVFLGRLK
                     RRTC"
BASE COUNT         1622 a         3367 c         3372 g         1561 t
ORIGIN      
        1 atgacacgca ccttctgcgt gggcgccgcg ccgccccggc tgcgcgaggt ctactcgcag
       61 gtcaaggaag tgctcatgtc cacgctggcc gagctggccg tgggcacgcg ctgcatcaac
      121 taccagatca aggcctgcga gcgcttcgag gccatggggt acgccaccat ccgccaggac
      181 agccgggtgg aatcgggata cgtgcacggc ctgggtcacg gcctcggcct cgatatccac
      241 gaatcgccac gactcggcgg cgcgctctcg aatcccgacg tgctggcccc gggccacgtg
      301 atcaccgtcg agcccggcct gtacctgccc gacgagggag tggcggtccg gctcgaggat
      361 gtcgtgtatg tccgtccgga tggtcgcatc gagaatctca ccacctatcc gtacgacatg
      421 gagatcttcc ctgagggata gcttcgacgt tcccgaggcg gccagtaccc gtcgccgctt
      481 ccacgacctg gaggccgaac gggcgcgccg cgagaaggcg gtgctcatcg gcgtggtgcc
      541 gcggaagact caggaagacg cgctggccga gctggccctc ctggccgaga cggcgggtgc
      601 cgaggtggtg aaggtcgtgc gccagcggcg cgatgtcatc tcgcccggca cgttcgtcag
      661 caagggaaag ctcgaagacc tgaaggccat ggccaattcc ggcgaggccg atctcttcat
      721 cttcgatgac gacctgcatc ccaaccaggt gaagcatctc caggacgcac tgcagaagaa
      781 gatcctggat cgctccgaac tgatcctaga catcttcgcg gcgcgcgccc gcacgcgcga
      841 ggccaagctc caggtggagc tggcccagct cgagtatctc ctcccgcgcc tcacccggat
      901 gtggagccac ctgtcgcgca ccggcggcgg catcggcacg cgcggacccg gcgaaacgca
      961 gctcgagacg gaccgtcggc tcgtgcgcga aaagatcggc gtgctcaagg agaagatgcg
     1021 cggcgtggag aaggagcggc aggtgcagcg caaccggcgg cgcaagcttc atcgcacggc
     1081 gctggtcggc tacaccaacg ccggcaagtc caccctgatg aacgccctca cgcgggccga
     1141 cgtcctggcc gaagacaagc tcttcgccac gctcgacgcc accacccgcc gcatggggtg
     1201 ggccgacggc acggtgacgc tgctgtccga cacggtgggt ttcgtccgca agctgccgca
     1261 tcacctggtg gcatcgttca agtccacctt cgaggaagtg gtggaggcgg acctgcttct
     1321 gcatgtggtc gatgcctcgc accccgatgc cgccccgcag atcgacgcgg tcgagcgcgt
     1381 gctggaggag atcggcgccg gctcgcgccg gacgctgctc atcctgaaca aggccgacag
     1441 tatcaccgac gaaacggagc tggccggcct ggcgctccgg catcccggtt cggtcatcgt
     1501 gagcgccctg cgcgcctcgg atcgcgagga actgcggcgg cggatccacg aggcggtgct
     1561 ggaaacgagt cgtcggccgg cggcggccgc cggcggaaat caatcgggag tgccgaaagc
     1621 ctcggcgtaa tcggccatgg aggctcggat cacctcgcgg gcgtgcgccg cgtcgtagcg
     1681 ccgggcgatc ctcaccggct ggtcgccctc gcgccccggc cggagcttgt aggtgaggaa
     1741 gtagtgctcg agccgctcca ccagggcggc cggcaggtcc gtcatctcgg ccacattgcc
     1801 ccacacgtaa tcgccggtca gcaccgccac gatcttgtcg tcggcctggc tcgcgtcgat
     1861 catctggagc ccgcccacca cccgggcccg caggaggatc tcgctccgcg cgatcggtcg
     1921 ttcgctgatg acgcagatgt cgagcggatc gccgtcgccg gccgtggagc cgggcgccag
     1981 agccgccacg cgcgccgcac agtaggtgcg cgggatgaag ccgtacagcg tggggggtga
     2041 cgacgagccc cgctgcggcc gatcgacctt gaggtagccg gtggccttgt cgacctcgta
     2101 cttcacgagg tcgaacggcg tgatctcgat ataggccgtc acgacatccg gcgcctccgc
     2161 ccccggctcg aggccgtgcc acgggtgggg acgatggcgc cggaatgatg gatccatggt
     2221 catgtgcggc cagtcgaggt catgtgcggc ctctggagat catgcgtagc gcatccgggt
     2281 catgcccggc cgaagacggt catcccgccc gcttgaaacg gagaagactc gattcgcccg
     2341 ggatctccag catcagctgg tcgcccgaca cgcgccatgc ggacacccgg gtgagcgcgg
     2401 cggtgaagcg atccgagagg ctgttggcgt tacagggcat gtcggtcatc cgcatgaggt
     2461 tgatgctgat ggatccgtcg ccgagcttgg acgggccggc cgccttgttg cagtccgagc
     2521 gcacgtggac gtagccgtcg ccgtcgaagc tgaccgtata gttttgcgga tcgatcaccg
     2581 acacattctc gctcccccgt gacaggtgga tccactccca ggtggtgccg cggagccggt
     2641 tcgggtcggt gtcgcccacg gcataggcgc cggcaaccgc ctgctggttg tcgtacgaca
     2701 tccgacccgt attgatggcc tggtcgttgg cgttggcatg ccgggaatcg ctggtgggcg
     2761 ccttcccgtc cgtgtgcagc gccatgtgtt cgcttctgta atcggggtaa ttcgagtggt
     2821 gcggtgcctg gctgcacccg gcccaggtga acgcgatggc tgccgtcatg agccacgcgg
     2881 caatgagtcg acggtgttgc ccggcctctt ccaggactga ctggagcatg tgatacctcc
     2941 cgctgtccga tcatcgatgc ttgggccaga cgaaaccagt ctagcagcga accgacgacc
     3001 gacggcaaga ggggagacgc gggcctccgg cgaatggggg attcccccat gaccggcccc
     3061 gcctgtttcc cgaaggtgta ttcgatcgga tctccgccct acttcacccg ccggaagcgg
     3121 agcacgctcg actcggccgg gatttccatg acgagctggc cgttcaccag gctccaggtg
     3181 gccacgcgcg tgagcagcga ggtgtagcgc gcgtcgagcg agccgggcgg gcaggccatc
     3241 cgcgtcagcc ggaggaggtt gatgctgatg gtgccatcgc ccagcttcac tggaccgcca
     3301 ccgcgattgc aggcggccag gatcgccacg ttgccgtcct cggcgaactc gagcgtgtac
     3361 ttcaccggat cgggcaccga gagattttca gttggcgaag agaaattcgt ccactcccag
     3421 ccggagccgg caaggccggc cgggttgcga tcgccgccga ccgtggctcc gccgggcccg
     3481 ccggtgcggg tcaacttgag cccggcggat ccccacatgt ccttgtcgaa gtcgaccgcg
     3541 gtcagggagt ttgcgtcggc ccggaagctc atcgtcgtgg gggccgcgtt gtgaccctcg
     3601 cgcttcaggg tcacctcggc catctctcgg ccgttcggcc tccaggttcc catggcaaca
     3661 atgggcggct ccccgttctg gtactcggtg gtcatccgtg ccgtcatgtc ggggttcagc
     3721 atcagcgtga tcgtccgtcc cggggacgag gctgccggca aggtggcctg ccagctgccg
     3781 gccagggcgt gcccaccggt ggtggtttcg gcggaagtgc cctgcttctg attgcagccg
     3841 gtcgacccca tcgggaggga cgccagacag agcacggcaa cgatggcccg cagggacggt
     3901 cgattgaaga gacgaggcac ggcactcctc ctggggatcg gtcgatgggg cgaccggtgg
     3961 atgtggacac tcggagtttg gacgagcggt tgatcaaact gttattgaat gaggttacag
     4021 ccggatagta accgaagagt caccacccca gtgcgcagcc gtccttccgc ttctccgtgg
     4081 cgccgacata ggtgccggtg accggatcac gccagatggc ctggtagccc ccgtagttgg
     4141 ccaccgcttc gtccttgatc acgtgccccc ggcgcctgag ctcggccagc accgcttccg
     4201 gaaggcccgg ctcgatgtgc aggatgcctc cgttggccat cacggtgccg gtcggttcgc
     4261 tcgagtcggt gtgatggaag cggatggcgt caccggcttc ctgcaggttc attccgaaat
     4321 cgaccaggtt gatcacgacc tgggcgtgcc cctgcggctg catgtcgccc cccatcaggc
     4381 caaacgccat gaggggcgtg ccgtccttcg tgaggaaggc cggaatgatg gtgtggaacg
     4441 ggcgtttgcc gggcttgagg ctgtttggat ggccgggatc gagcgagaag agcgcgcccc
     4501 ggttctggag gccgaaaccg agctcgggaa tcacgtagcc gcttccgaag ccggtgtagt
     4561 tgctctggat gaggctgatc atcatcccgt cggcgtcggc ggcgcagagg aaggtggttt
     4621 cgcggcgatt cagcgcggcc ggggcggccc cgccgggctc gccgggcgcg tcggtggcgg
     4681 cggctttcgt catgtcgatg tggggggcgc gctcgcgtgc gtattccttg tcgatcaaac
     4741 ccgccactgg caccgcggcg aaggcggggt cggcgtagta gcgtgcccgg tcggcaaagg
     4801 ccactttctt ggcctcggtc aggaggtgcc agaaatcggc cgactcgcgc cccatgccgg
     4861 ccacgtcgaa cgtctccagc atgttcagca tctggagcgc ggcgatcccc tggccggggg
     4921 gcggcagttc ccagacgtcg tagccgcggt agctggcgtg gaccggggca tcccaggtcg
     4981 aggtgtgctt ctcgaagtcc acccgcgcca agaagccgcc gttggcctgc gaatagcgca
     5041 cgatggcgtc ggtgatgggg ccggcgtaga aggcctcgcg tccccccgca gcaatcgcgt
     5101 cgagagtccg ggccagcgcg gggttccgga aaatctgtcc ggcgctgggc gcctcaccgc
     5161 ccggcatgaa gacttcggca taccccggct tgtcgcgaaa gcgggggaga cccgccgccc
     5221 agttggccgc gatgaccggc gagaggggga acccctcgtt ggcgtagcgg atggcgggcc
     5281 cgaggagttc gctcatcggg agcttgccgt agcgctcgtg cagcgcgaac caaccgtcca
     5341 cgcagccagg taccgaccat gaatacggcg agaagagcgg aatcgtgccg tcggagtcgg
     5401 ggggcacttt ctcgatggtg agcgccatcg gggcccgccc cgatccgttg agcccgacga
     5461 gctttccctc cgtcggatcc cacacgatgg cgaagagatc gcccccgagg ccgcacgacg
     5521 taggctccat gagcgcgaga caggcgttga cggcgatggc ggcgtccacc gccgtgcccc
     5581 ccttcttgag cacatccacc ccgacctgga cggccagcgg ctgggccgcc gccaccatgc
     5641 cgtgcgccgc cacgaccgga ctccggctgg cgtcgccgcg gccgatcggt tggatctccc
     5701 caccgcttgc gctcgaagtc acgagcgccg ccactccgag aagaaagaca cgagtcgcca
     5761 catcatgccg tgaggacatc cgtccgctcc acctggccgc cgcaccaccc ctgcgcgcgt
     5821 tcggcgaatg tttccaccgc ctcggccagc tcgtcctccg gaaagccgac gggcacgtag
     5881 aggagcgcca acgcgtgcgt ggtttcggtg gaaaccgccg tgcgatacga atccgaagtg
     5941 ggcgatccga acgcgccatg gtggtcggcg gccaccagcc gaccttcgag gttgatgatc
     6001 ccgcggccga tcccttcgta cgcctcgccg gccttcccct gccggagcac gacacctccc
     6061 tcgatccggg gcaccgaata gagtcccatc ggcaccaggt actccagcga gcaccagttg
     6121 agcgcgtcga ccagcgtgtt cacggggtga agcggctctc ccttgagcgc ccggcggagg
     6181 agcgcttccg aggaggggcg atgcttggtc gggtcgatgc cgaccgacga gtagagcttc
     6241 cgcgcccact ccacgcccgg cacgctcccg atcggggccc cgccgaactc gtcccggagc
     6301 tcggcgcaga gcgcggtgat cacgtcccag cgagggtttt cggtgtggac ggtcaggccg
     6361 gtcatctcga cgagaccaac ctgaagaatt ccggccagcg agggatcgat cgtcacgttg
     6421 accatggccc ccgagtatcc ggtcgggcta ctccaggcgc aagagctgga agaggcggat
     6481 tccggcgctt cgatgcgcct cggaaaattg cgggtgagcc tctccccacg cctcgaacgc
     6541 ggcggcgccg acatcctccg cgcggacgat cacgaagacc ggcgcgccgg ttgccgccag
     6601 ggggcccgtg tcgatggcgg gtccgtttcg cgcggcgatg cctgacggcc cgaccagctg
     6661 atcgcgcagg taacgccggc gggccatctc tccctcgtca tagccccact gctcggcgta
     6721 ggcctcgcgc ccccagaact ggcggcgcgg cacactcacg agcacgtcgc agcgctcgcg
     6781 agagtcgagc acgatggcgt cacgcggggt gttggcgcgc agccagtcgt atgcctcgac
     6841 cacctcgacc gggcgcctcg agtaatccgg ctcgccgagg taggacgccc atgccaggag
     6901 attggcgggc gccaggatcg ccagcgccgc gaggaggccg gccatcccgc gccagcgaga
     6961 ggagagcgcc acaccggcca ggatggccgg cggcaggtag aggagatagg tgaacttgtc
     7021 ggtggtgttg ggacccggca ggcggatgaa cgacgcgaag accagcgtgg cgatgatcca
     7081 ggtgaccagc atccgtcccc acggtgtgcc ctcgcgccac aggcggcgtg cgagtccggc
     7141 ggcagcgatc gccacgaaca acacacccac caggagtccg gccacgttca cccagtcgaa
     7201 gtggatcggc acgagttggc cacccggctt gccacgcgtc acgccgacga gatacggcac
     7261 ggccaggagc aggctcacga cgaccgatcc gccccacgcc gccaccgcgc cgaacgactg
     7321 cccgccgcgg cgcgggccaa ggaccagcag caccacgccc gtgatgcccg acacggccac
     7381 ggtcgtgaaa ccgacgaccg ggtggaacat caaggccagg aagagcgccg gcatcaccac
     7441 gaggaggggc ttgagtcggg gccgttcgat gaagctcagc acggccgcga ggtagacaag
     7501 gaacgccacc agcgccaggc cgtaggccgt ccccaccagg aacttgttga ggaagaaggg
     7561 cacgctcccg aaatcggaga gaaacgttcg tatcgtgccg atgtcgaggg ggcgccagcg
     7621 gaacgcctcg gcgagctgct ccatgccgcc gctctttccg gcgagcgcct tgagcgcgcg
     7681 gatcggcatg acgagccaga cgaggacgcc gagcccgagc ggcaccacgt atgccgcggt
     7741 ccgggccgcg cgcgccggtt catcgcctgt ccgtcgggcg atccactcgg ccaggtgcgc
     7801 cgacacaagg atcaacgcca tgagtgcgag ggcgttcacg aggaccatca aggtgaacgt
     7861 gtcgaccggc gccactttgc gcacggccgc cagcatcgcg tggaagaacc acatgtactg
     7921 gagcggcagg ccggcgaagt aggggtcctg cggcggcaca ccggcccgtt cgatctcggc
     7981 cacgatggcc gcgtgaaacc acgagtcggc gcgcgtgcgg atccagggat ggagaatcgc
     8041 gggcaggatc gtgagtcccg tcagggccag ggcaatcgga agggccccgc gggtatcgcc
     8101 gtcgtgcggc cggtcgggcg aagctggttt gacagtcttc aacgggcgtt catactatcc
     8161 gaaatggcca acttcgacct gatgcgccgc ctgcacacca cggacaatcc ggcccgcatc
     8221 atcctcctgg tgatggatgg cctcgggggc ctgccacgcg agccgggcgg caagaccgag
     8281 ctcgagacgg cgcgcacgcc caacctcgat gccctctgcg cccgcggttc gtgcgggctc
     8341 tcgtatcccg tggcccccgg catcacgccc ggctccggcc ccgcccacct ggccctcttc
     8401 ggctacgacc cgatcacgtt cgacatcggc cgcggcgtgc tggaggcact cggcatcgag
     8461 ttccccatga cgccgcgcga tctcgcggcg cgcggcaact tctgttcgct cgacgcggcc
     8521 gggaacgtca ccgaccgtcg ggcgggacgc atcgacacgg aaatctgcgt ccgcctctgc
     8581 agcgagctct cggccagggt tcgcgtgccg ggcgtggagg tgttcgtcga gccggtcaag
     8641 gaacaccgct tcgtggtggt gctccgcggc gacggcctcg aggaggggct gaccgaaacc
     8701 gatcccctgg tcaccggcaa gccgccgctg gccatcgagc cggagtcacc cgccgcggcc
     8761 gccacggcca cgctgttcaa cacgttcgtt cgcgaggccg gccgggtgct cgccgagcac
     8821 catccggcca acggtgtcct gctccgcggc ttcgcgaagc accccacgct ccccaccatg
     8881 agcgagctgt acggcctgaa ctgtggcgcg gtggccgtct acccgatgta ccggggcctc
     8941 gcccggctgg tcggcatgag caaggtcacg ttcgaagggg agtcgccggc cgacgagttc
     9001 cgcgcggtgg cggccaactg ggaaaagcac gacttcttct tcatccacgt gaagaagacc
     9061 gactcgtacg gcgaggacgg caacttcgac gccaaggtcg gcgtcatcga gtcggtcgac
     9121 caggcactcc cgatcctgct cgagaagaaa cccgaggtga tcatcgtgac gggcgatcac
     9181 tccacgcccg ccaccatgaa gacgcactcg tggcacgggg tgcccacgat catcgcttcg
     9241 ccctgggccc gcttcgacgg caccacgtca tacggcgaat cggcctgcgc ccggggcggg
     9301 ctcggtcact tcccggccgt gcacctcatg ccgctggccc tggcccacgc ccgaaggctc
     9361 aacaagtacg gcgcttgagt cgtcccagga aaaccttgat ctccctggcc gtctgcaggt
     9421 cgccggtggt gtccgcgacg ttcaacccgg tcgtgtacgc cacttcggct tcgctcgtcc
     9481 gacctgccga cgccagcgcg ttgcccagct cgcggtagct cgccgtgtga tccggcgcca
     9541 gccggatggc ctccgccagt tgttcggccg cctcgttggg gaggtcggcc gacaggagtt
     9601 cctttcccag catgaaacgg gcaagcccgt ccttcggatc ctcggccacc atctgttgca
     9661 acgattcgag ccgattcata tgtgttcctc tcaggatgcc gccggtctcg tctgtgtatg
     9721 ggcgcggccg cgggtgtccc aatggtacac tggcccggaa cgcttggccg aaaaggagaa
     9781 aacaacgatg atccaaaccc cgctgtcgtt ccgcgccgtt ccgcccgcgg cccgtctcct
     9841 ggccttggta gccctgatgt gcgccctcgt tctctccccg accccggccg cccgtgccgg
     9901 cggcgaagtg gggaccgtgg cc
//
LOCUS       DASVOD010000548         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_922305, whole genome shotgun sequence.
ACCESSION   DASVOD010000548 DASVOD010000000
VERSION     DASVOD010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922305"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF720_12145"
     CDS             <1..535
                     /locus_tag="VF720_12145"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX7880156.1"
                     /translation="TQAGAWGFYHFDDEGWTAGPTRIMENGIFKRGLSDSWSARLSKV
                     PRTANGRRQSWDHKAYARMSNTFFEAGTQSREELIRGVSKGILVGHFTAGIEDPKGWG
                     MQVVCQYGEKIENGKLTGKVVSPVTLSGYVPDVLASVDGVGNDFELSPGTCGKGYKEF
                     VPITSGGPTIRFKARVS"
     gene            538..1839
                     /locus_tag="VF720_12150"
     CDS             538..1839
                     /locus_tag="VF720_12150"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX7880157.1"
                     /translation="MPPKPGNPIERLKKTPGISGWVVSSRRLRRDELYFQGMATESRR
                     SIENLGGRVELFTTNGQRMGRGSFDILAADPAAFDTQVADAIARAKVGSELPFPLSGP
                     ATYGKVNLLDPEVRDAAENMPSRARSAILEGLGRTKGVRLAAAEVFVTKTVTAVVNSA
                     GAAAENDESNYNIELVLLAGSGASEQERQARWRRRRFEDLSLTVAVQEEAERAIDRLS
                     ATLPTAGSTPVIFGPELLDDMLGFLLPLASASTLYRKDGPLTLGQPIADAGSDPGDPL
                     IVEVDALFPFGPASYRIDGLGTPGGNVRLIEKGIFVHPHADAQFAHYLKLDKPTGAPG
                     TIQLPAGTTPLADLQRDRYLEVVAVSDLIPSRGSGRFSAEIRLGYEVSGGTRRPVTGG
                     TLTGNVFEALSRARWSKETALHDRYVGPTWARVEQGLTVIA"
     gene            1836..2987
                     /locus_tag="VF720_12155"
     CDS             1836..2987
                     /locus_tag="VF720_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880158.1"
                     /translation="MTGRQLALAVTSFLLALLAPEARGTRILVPESMSLSAAVNAAAP
                     GDTLSLATGTYERIPDPFVKSIVIEARQPEDPPVLRRLEAGLVTLRHLDFVPEGPGEV
                     NPILFRALDSVRLEGCRFSGFASTGIDARDAPGDHAWLVSVEACRFNLLNRGIDAAFV
                     NPGSRIDVHDSAFESSFYGIRMATTARQCPGGAGSEPPSLTGMAALNLLDCRFTTLNG
                     SAVEAHEGTWAVSMSNCRIEGTKLGVELIRAGASLERTEIIGNGFDSIGIQANSSRIE
                     VASSILQQHALAIGLTAASGCERPAGIIGGQIEHFCRLNGNVVALATSHPVNADANWW
                     GTLICSEAHERIQGMTIQTLVDDAGSFEVECMTVVTPTTWGRLKARIAP"
BASE COUNT          493 a         1030 c         1022 g          465 t
ORIGIN      
        1 gacccaggcc ggcgcgtggg gtttctacca cttcgatgac gaaggatgga cggcgggtcc
       61 gacgcgcatc atggaaaacg gcatcttcaa gcgcggcctc tcggactcgt ggagtgcccg
      121 cctgtcgaag gtgccccgca ccgccaacgg gcggcgccag agctgggatc acaaggcgta
      181 tgcgcgcatg agcaacacct tcttcgaggc cggcacgcag tcgcgcgagg aactcatccg
      241 gggcgtgtcg aaggggatcc tggtcgggca cttcaccgcc ggcatcgagg atcccaaggg
      301 gtggggcatg caggtcgtgt gccagtacgg cgagaagatc gagaacggca agctgaccgg
      361 caaggtggtc tccccggtca ccctttccgg ctacgttccg gacgtgctgg cctcggtgga
      421 cggcgtcggc aacgacttcg aactctcgcc gggcacctgt ggaaaagggt acaaggagtt
      481 cgtgccgatc acgtcgggcg ggcccacgat ccgattcaag gcgcgggtgt cgtaggcatg
      541 ccgccgaaac cggggaaccc gatcgagcga ctcaagaaga cccccggcat ttccggctgg
      601 gtggtgtcga gccggcggct ccgccgcgac gagctctact tccagggaat ggccaccgaa
      661 agccgccgat cgatcgagaa cctgggagga cgcgtcgagc tcttcactac caacggccaa
      721 cggatggggc gcggctcctt cgatatcctg gccgccgatc cggccgcgtt cgacacgcag
      781 gtggccgacg ccatcgcccg cgcgaaggtc gggagcgaac tccctttccc gctcagcggc
      841 cccgccacct atggcaaggt gaacctgctc gaccccgagg tgcgcgacgc ggccgagaac
      901 atgccgtcgc gcgcccgttc ggccatcctc gaaggactgg gacgcacgaa gggggtgcgg
      961 ctggcggcgg ccgaggtgtt cgtgaccaag acggtgaccg ccgtggtcaa ctcggccggc
     1021 gccgcggccg agaacgacga gtcgaactac aacatcgagc tcgtgctcct cgcgggatcg
     1081 ggtgcttcgg agcaggagcg acaggcacgc tggcggcggc ggcgcttcga ggacctgagc
     1141 ctgacggtgg ccgtgcagga ggaagccgag cgggccatcg atcgtctgtc ggccaccctt
     1201 cccaccgcgg gttcgacgcc ggtgatcttc ggtccggagc tgctcgacga catgctggga
     1261 ttcctgttgc ctctggcgtc ggcgtccacg ctctaccgga aggacggccc tctgacgctg
     1321 ggacagccga tcgccgatgc ggggagcgac ccgggcgatc ccctcatcgt ggaggtcgac
     1381 gccctcttcc cgttcgggcc ggcgtcgtat cgcatcgacg gactcggcac gccgggcgga
     1441 aacgtccgcc tgatcgagaa ggggatattc gtccacccgc acgcggacgc gcagttcgcc
     1501 cattacctca agctcgacaa gccgaccggc gcgcccggca ccatccagct cccggccgga
     1561 acgaccccgc tggccgacct gcaacgcgac cgttacctgg aagtggtggc ggtgtccgat
     1621 ctcatcccct cgcgcggcag cggccgcttc tccgccgaga tacgcctcgg ctacgaggtg
     1681 tcgggcggga cgcgtcggcc ggtgacgggc gggacgctca ccggaaacgt tttcgaggcg
     1741 ttgagccggg cccgctggtc gaaggaaacc gccctccacg accgctacgt ggggcccacg
     1801 tgggcccgcg tggagcaggg tctcacggtc atcgcatgac cggacgtcaa cttgcgctgg
     1861 ccgtcacttc cttcctgctc gccctcctcg cgccggaggc gcggggtacg cgcatcctgg
     1921 tccccgagtc gatgtccctc tcggccgcgg tgaacgccgc cgcgcccggc gacacgctct
     1981 cgctggccac cggcacctac gagcgcattc cggacccgtt cgtgaagagc atcgtgatcg
     2041 aggcccgcca gccggaggac ccgccggtgc tgcgccgcct ggaggcgggg ctcgtgacgc
     2101 tccgtcatct cgatttcgtg ccggaggggc cgggcgaggt gaacccgatc ctcttccggg
     2161 ccctcgattc ggtgcggctt gaagggtgcc ggttcagcgg atttgcctcg accggcatcg
     2221 atgcgcgcga cgccccgggg gatcatgcct ggctcgtctc ggtggaggcc tgccggttca
     2281 acctcctgaa ccgggggatc gatgccgcct tcgtgaaccc cggaagccgc atcgacgtgc
     2341 acgactcggc tttcgaatcg agcttttacg gaatccggat ggccaccacg gcgcgccaat
     2401 gcccgggtgg ggcggggtcg gaaccgccat ccctcaccgg catggccgcg ctgaatctcc
     2461 tcgactgccg cttcactacg ctgaacggct ccgcggtgga agctcacgaa gggacctggg
     2521 cggtctccat gtcgaactgc cgcatcgagg ggacgaagct gggggttgag ctcatccggg
     2581 cgggcgcctc gctcgagcga accgagatca tcggcaacgg gttcgattcc atcggcatcc
     2641 aggccaattc gagccgcatc gaggtcgcga gctccatcct tcagcagcac gcgctcgcca
     2701 tcggcctcac ggccgccagc ggttgcgaac gaccggccgg catcatcggc ggccagatcg
     2761 aacacttctg ccgcctcaac ggcaacgtcg tggctctcgc cacctcgcac ccggtgaacg
     2821 ccgatgccaa ctggtgggga acgctcatct gcagcgaggc gcacgaacgg atccagggga
     2881 tgacgatcca gaccctcgtg gacgacgccg gatcgttcga agtggagtgc atgaccgtcg
     2941 tcacgccgac cacctggggc cgcctgaaag ctcgaatcgc cccatgaagc acctcgtgcg
     3001 gctgaagccg
//
LOCUS       DASVOD010000549         3464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_192670, whole genome shotgun sequence.
ACCESSION   DASVOD010000549 DASVOD010000000
VERSION     DASVOD010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192670"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1321)
                     /gene="rnd"
                     /locus_tag="VF720_12160"
     CDS             complement(110..1321)
                     /gene="rnd"
                     /locus_tag="VF720_12160"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX7880159.1"
                     /translation="MSLYIDDTARLQRLCARLSAVPSFGLDLEFQRERTYYPRLCLLQ
                     VALPGRADQAMAADGAERLAIVDPQVVRDMSAFWELVADPAIEKVLHAGRQDMEIAWG
                     HGGILPRNIVDTQIAAAMIGAGDQPGYGTLVERFCGVRLSKTETVTDWSRRPLTPEQL
                     DYALDDVRYLHDIESTIRRRLDALGRRDWAREEMAHYEEEATWNPDLSRLFLKLPRLR
                     SLPRKSLVVARELAIWREEEARRRDEPRNRIVSDEVLVEVARRLPKTQAQIDILRGLH
                     PNEARRVGPALLEVVARAMAIPESEWPELRAIPAEDTERALVVDLLEVFLRSRARESE
                     IAPSYLGVKADLTDLVDEVRGRREADREAPLLLTGWRREMVGDDLVAIAQGRVNVHVD
                     PATGHVTTSDR"
     gene            complement(1318..1923)
                     /locus_tag="VF720_12165"
     CDS             complement(1318..1923)
                     /locus_tag="VF720_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c-553"
                     /protein_id="HEX7880160.1"
                     /translation="MLRRNLPRLLLLGLLVVVAGCQTGTPETGSNTIPAATPPPVDPA
                     TRGKYLVTILGCNDCHTPMSGMGPDGPIFDQTHFLAGHPAALVMPPPPAPVGPWFAAT
                     NLTAWSGPWGISYAANITPDSLTGIGIWTEDVFIKALRTGRHVGVARPILPPMPWQSF
                     AHMTDEDIKSVYAYLRTIPAVSNEVPQPVINGPPPAAEGTH"
     gene            2091..2609
                     /locus_tag="VF720_12170"
     CDS             2091..2609
                     /locus_tag="VF720_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880161.1"
                     /translation="MTVADFAGLPDTARLYIFGSSRPLSPEETTRFLAAADEFLAGWQ
                     AHKADLVAAREWRHDRFLLVAADEEVTALSGCSIDSLVHRIKQFEGELSMRFLEGGAV
                     FFRRGDDIVRIGRSDFIKLVEKGEVGKGTMVFDNTITTLGALRAGRWELPFERSWHAE
                     LAPPPSTISTQG"
     gene            2606..>3464
                     /locus_tag="VF720_12175"
     CDS             2606..>3464
                     /locus_tag="VF720_12175"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX7880162.1"
                     /translation="MTARPSGWLEVDTPQTHPDNARRLMAAHPEIRKLIGHNPWSFLP
                     LAGIVIVQTGLAIWFGRNDTAWWIIALTAWFFGAILHHGLWVLLHESGHMLLFGKRKW
                     NHMAGMLSNLPLLCPAYFNFTKSHRRHHLALGEYDEDADLAANFEAKLVGNDFVRKSL
                     WLVLFPFFQGLRPFHTPPPDYINAAVLSNIAAQAAYVTGVAFIGGWGAILYLLLSLFF
                     SIGLHPLGARWIQEHYTLEADQETFSYYGWGNKLAFNVGYHNEHHDFPSIPWNRLPEL
                     RRIADEHYGK"
BASE COUNT          541 a         1154 c         1175 g          594 t
ORIGIN      
        1 cccgtcagcg gcgggatgcc gatcaggcca acccggatga cggcccacgt ggacccccag
       61 atgagcgtgg gtatggcaat gatcaggagc gtgagttttc ggccggggtt caccggtccg
      121 acgtggtgac gtggccggtg gccggatcga cgtgcacgtt cacccgtccc tgtgcgatgg
      181 ccaccaggtc gtcacccacc atctcgcggc gccaccccgt caggaggagc ggcgcctcgc
      241 ggtcggcctc gcgacgaccg cggacctcgt cgaccaggtc ggtgaggtcg gccttcacgc
      301 cgaggtacga aggggcgatc tccgattcgc gggcccggga acggaggaag acctcgagga
      361 gatcgacgac gagcgcccgc tcggtatctt cggccggaat ggcccggagt tcgggccact
      421 cggattcggg aatggccatg gcccgcgcca ccacctcgag gagggcaggc cctacccggc
      481 gagcctcgtt cggatggagg ccgcgcagga tgtcgatctg ggcctgcgtc ttcggcaggc
      541 gtcgggccac ctccaccagc acctcgtcgg aaacgatccg gttgcgcggt tcatcgcgcc
      601 ggcgggcctc ctcctcgcgc cagatggcca gttcgcgcgc caccaccagg ctcttgcgcg
      661 ggagcgagcg gagccgcgga agcttgagga agagtcggga gaggtcggga ttccaggtgg
      721 cctcctcctc gtagtgggcc atttcctcgc gcgcccaatc acgccggccg agcgcgtcga
      781 gtcggcggcg gatggtgctc tcgatgtcgt gcaggtagcg gacatcgtcg agggcatagt
      841 cgagctgctc gggcgtgagc ggacggcgcg accagtcggt caccgtttcg gtcttcgaga
      901 ggcgcacgcc gcagaagcgc tccaccagcg tgccgtagcc cggctggtcg ccggcgccga
      961 tcatggccgc ggcgatctgc gtgtcgacga tgttccgggg gaggatgccg ccgtgccccc
     1021 aggcgatctc catgtcctgg cggccggcgt gaaggacctt ttcgatggca gggtcggcca
     1081 ccagctccca gaaggcgctc atgtcacgca ccacctgggg atcgacgatg gccagtcgtt
     1141 ccgcgccgtc ggccgccatc gcctggtcgg cccggccggg gagtgccacc tgcaggaggc
     1201 agaggcgggg gtaataggtg cgttcccgct ggaattcgag gtcgagaccg aaagacggca
     1261 cggccgagag ccgcgcgcag aggcgctgga gcctggccgt gtcgtcgatg tagaggctca
     1321 gtgcgtcccc tcggcggcgg gcggcggtcc gttgatcacc ggctgcggta cttcgttgct
     1381 caccgccggg atcgtccgga ggtaggcgta cacggacttg atgtcctcgt cggtcatgtg
     1441 ggcgaacgac tgccacggca ttggcggaag gatcgggcgg gccacgccga cgtgacggcc
     1501 ggtgcgcagg gccttgatga agacatcctc ggtccagatc ccgatgccgg tcagcgagtc
     1561 aggggtgatg ttggcggcgt agctgatgcc ccacggcccg gaccatgccg tgaggttggt
     1621 ggcggcgaac caggggccga ccggcgccgg cgggggaggc atgacgagcg ccgccggatg
     1681 gccggccagg aagtgggtct gatcgaagat gggaccgtcg ggccccatgc cggacatcgg
     1741 ggtgtggcag tcgttgcagc cgaggatggt aacgaggtac ttgccgcgcg tggccggatc
     1801 gaccggcggc ggcgtggccg ctggaattgt attcgagccg gtttcgggtg tgccggtctg
     1861 gcatccggcc accacgacca ggagtccgag gagcagaagt cgagggaggt tgcgacgcag
     1921 catggggacc tttcctccgt ggaacgacga cgcgcacccg gacgggcgtc cggtctggcg
     1981 ggaagatggc gcgaaggtcg gggcaagtca agaacggaac gtccccgggg gctctcgtgt
     2041 atagtagtcg cctgcgtcac cgtttttgtc tacacccttg ggactcgatc atgaccgttg
     2101 ccgacttcgc cggacttccc gacaccgccc gtctctacat tttcggatcg tcacgccccc
     2161 tctcgccgga agaaaccacc cggttcctcg ccgccgccga cgaattcctg gccggctggc
     2221 aggcccacaa ggccgatctg gttgccgccc gcgagtggcg tcacgatcgg ttcctcctgg
     2281 tggccgccga cgaagaggtg acggcccttt ccggctgctc catcgactcc ctggtccacc
     2341 ggatcaagca gttcgaaggg gagctgtcga tgcgattcct ggaaggtggc gccgtctttt
     2401 tccgccgggg cgacgacatc gtccgcatcg gccgctcgga tttcatcaag ctcgtcgaga
     2461 agggcgaggt tggaaagggg acgatggtct tcgacaacac gatcaccacg ctcggcgccc
     2521 tccgcgcggg gcggtgggaa ttgccgttcg agcgttcctg gcacgccgag ctcgctcctc
     2581 caccttccac gatttccacc cagggatgac ggcgcgcccg agcggttggc tcgaagtcga
     2641 caccccgcag acgcaccccg acaatgcccg gcggctcatg gccgcccacc ccgaaatccg
     2701 caagctcatc ggccacaatc cctggtcatt cctgccgctg gccggcatcg tcatcgtgca
     2761 gacgggcctg gccatctggt tcggccgtaa cgacaccgcc tggtggatca tcgccctcac
     2821 ggcctggttc ttcggcgcca tcctgcatca cggcctctgg gtgctgctgc acgagtcggg
     2881 acacatgctc ctcttcggca agcggaagtg gaaccacatg gcggggatgc tctcgaacct
     2941 tccgctcctc tgcccggcct actttaattt caccaagtcg caccgccgcc atcacctggc
     3001 ccttggcgag tacgacgaag atgcggatct cgccgccaac ttcgaggcca aactggtcgg
     3061 caacgacttc gtgcgcaagt cgctctggct ggtcctcttc ccgttcttcc agggactccg
     3121 gccgttccac acgccgcccc ccgattacat caacgccgcg gtcctttcga acatcgcggc
     3181 gcaggcggcc tatgtcaccg gcgtggcgtt catcggcggc tggggcgcca tcctctatct
     3241 tctcctctcg ctcttcttct ccatcggcct tcatcccctc ggcgcccgct ggatccagga
     3301 gcactacacg ctcgaggccg accaggagac gttttcgtac tacggctggg ggaacaagct
     3361 ggccttcaac gtggggtatc acaacgagca ccacgacttc ccctcgatcc cgtggaatcg
     3421 tctgccggag ctgcgccgga ttgccgacga gcactacggc aagc
//
LOCUS       DASVOD010000550         6777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_297298, whole genome shotgun sequence.
ACCESSION   DASVOD010000550 DASVOD010000000
VERSION     DASVOD010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297298"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF720_12180"
     CDS             complement(<1..870)
                     /locus_tag="VF720_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7880163.1"
                     /translation="MAVRDISFSVARGEVLGFLGPNGAGKTTTMRILTSYLPADDGQV
                     EVAGFDVATQSLEVRRRLGYLPENAPLYEDMGVIDYLEYVAGMRGYTGAERTRRVRRM
                     LDVCGLGTMMQKMIGQLSKGYRQRVGLAQAMIHDPDLLILDEPTSGLDPNQIVEIRDL
                     IRDVGKEKTVILSTHILPEVTATCSRVMIIAGGALVADDTPEGLARKAAGGQEIRAVI
                     KGPGPDIEATLRAAEDQLGPARYEGSAPGGLAYRFSIPSGVDAHVLAEVIFRLAADKG
                     WALTELSHDTASLE"
     gene            1133..1915
                     /locus_tag="VF720_12185"
     CDS             1133..1915
                     /locus_tag="VF720_12185"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HEX7880164.1"
                     /translation="MLALAFASPATAKGPARQTAPPPPLVTGPTYEWADFDSLLDRYV
                     VGGRVNYAAWKADGSAGLDKVLAAAGAWAMYDSATSEEKVVFLSNAYNGFVIQGVLAA
                     YPVKSVKDIPGFFDKATHDIAGGHYTLDALEKTLLKPLGRKLNDPYYHMALVCGSVGC
                     PALRNRAYRQATWQGETMNQAQHFVADPTKLQFEEESNTLRVSELFDWYISDFESGDT
                     TLPRWIGPHLSLGAAMKMSATEPIVEYLPFDWALNDVPAGSK"
     gene            1912..3102
                     /locus_tag="VF720_12190"
     CDS             1912..3102
                     /locus_tag="VF720_12190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX7880165.1"
                     /translation="MTRVTTAPRTGEVLLGEIGWARFKAGHPWIYRGQFKSPAADLAA
                     GTVRIVDPRGKVLGTALYSPASQIALRLLTREETTIDAAWFASRFSAARDYRDRVMEP
                     RSARREFFSESDGIPSLVVDRYGDHLVVQVLSAGLEALEGALFEALGEVYKPASILAR
                     NEVSVRKLEGLERGVFQKHGVTPERIEIQEGERTLEVDPWRGQKTGLFLDQFENHAAA
                     TQLTRGRVLDAFASTGGFGLAMSGRAREVVALDSSGDSLATAARNVTRNGLDNFKFVE
                     ANAFDWLKSADQAGEKFDVIVLDPPAFAKNKAEVEAGVRGYKEINLRAMKMLTPGGVL
                     VSCSCSHHITEELFHDVLAAAAADVRRRFRIIEKRTQGRDHPILVGFPESYYLKCFLL
                     QFLD"
     gene            3128..4372
                     /locus_tag="VF720_12195"
     CDS             3128..4372
                     /locus_tag="VF720_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEX7880166.1"
                     /translation="MTDRDSLIHDWNLADGPPPRPSHAIEFDDETLRDGLQSPSVTDP
                     PIEKKIEILHLMERLGIQTADIGLPAAGPRARADVKRLAEEIVRSKLRIGANCAARTK
                     PEDIDPIADVSQAVGIPIEACTFLGSSPIRMYTEGWTVDQLLQLTETAVRYGVSKGLP
                     VMMVTEDSTRAHPETLRRIYTTAIECGAKRVCVADTTGHATPEGTAALIRFILGVVAD
                     TGEDVKVDWHGHNDRGLGTVNCIAAAAAGAHRIHGTALGVGERVGNAALDQVLVNFKL
                     LGWIDNDLSALGQYCETVSRSTDVPIPVSYPVVGRDAFRTATGVHAAAVIKAARKGDT
                     WLTDAIYSGVPAGMFGLEQVIEIGPMSGASNVSFWLEKRGYPANDELVQAIFGEAKSA
                     ARVLTDAEVHAICRTKGVKAGA"
     gene            4369..5538
                     /locus_tag="VF720_12200"
     CDS             4369..5538
                     /locus_tag="VF720_12200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7880167.1"
                     /translation="MSRPQGDRSRLYAAAFLRSLATGLSGVLLGLYLARVGLSATTIG
                     YAISAGLGGGALAATITAFFGDRIGRRRLLVAVNLLGAIGGLVLYFGDSPWLLIAASF
                     AGMVNGMGRDRGATLILDQAILPGTTDNTGRTKVFAVYNVFQDIGHAVGGLLAAIPDM
                     RSAFLVYSGLLLAAAILDMTLSSAVEAAEPTRRPTLSPKSRSLITRISALFAMDSLGG
                     GFLTTSLLAYFFHERFGVSAGTIGFLFFAARVANAGSHLAAAWLATRIGLVNTMVFTH
                     LPSSLLLLTVPFAPNFTVAAILFLLRESLVEMDVPTRQSYVMAVVAPEERTVASGITH
                     LVRLTAWAVAPAFAGAMMTGALGAPLAAGAGMKIAYDLLLWRAFRKVRPPEEHPA"
     gene            complement(5561..5875)
                     /locus_tag="VF720_12205"
     CDS             complement(5561..5875)
                     /locus_tag="VF720_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880168.1"
                     /translation="MVDHPIPFDAEAGHRYAEITPNWLVGDILNLFPGAAAILDRHFL
                     AGCPEHPGCSGCPGRFVDSLEEAAWLSGAEEHLEYLVAELNEAYRQWVDGETFWGVAG
                     RA"
     gene            5943..>6777
                     /locus_tag="VF720_12210"
     CDS             5943..>6777
                     /locus_tag="VF720_12210"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX7880169.1"
                     /translation="MRNSNWIRWGLGAALLLTGGCGRKSNTEWVRPAPTPLTTDARFD
                     GEPSLSPDGRQVVFVSFGESDLDLFVIDPARPSERRLLHGGPGDDRQPAYSPDGKRLA
                     WTLVTGDDSDIMMTGEGGDARRVTVEAGHDQQPAWSADGTRLAFVSDRTGRTEIWVTT
                     LSTGQSAPISAASPPSNDYVLADPAWRGEAVVVSATVDDQVDLWEIAATGGAWRRLTA
                     GPARERHPDPGPGDRLAFTSDTTGYANLYVREPDGRITALTDERTDIFESRWTSDGSR
                     IL"
BASE COUNT         1084 a         2320 c         2253 g         1120 t
ORIGIN      
        1 ctcgagactg gcggtgtcgt gcgagagctc ggtgagcgcc catcccttgt cggcggccag
       61 gcggaagatc acttcggcca ggacgtgggc gtccactccc gaagggatgc tgaagcggta
      121 agccagcccg cccggtgccg agccttcgta gcgcgcgggg ccgagctggt cttccgcggc
      181 gcgcagcgtg gcctcgatgt cgggccccgg tcccttgatg actgcgcgga tttcctggcc
      241 gccggccgcc ttgcgggcca gcccctcggg cgtgtcgtcg gccaccaggg cgccgccggc
      301 gatgatcatc acgcggctgc aggtagcggt gacctcgggg agtatgtgcg tggaaaggat
      361 gaccgtcttc tccttgccga cgtcgcggat caggtcgcgg atttccacga tctggttcgg
      421 gtcgaggccg ctcgtcggtt cgtcgaggat caacagatcg gggtcgtgga tcatcgcctg
      481 ggccaggccg acgcgctggc gatacccctt ggagagctgc ccgatcatct tctgcatcat
      541 ggtgcccagg ccgcaaacgt cgagcatgcg gcggacacgc cgggtgcgct ccgctcccgt
      601 gtagccgcgc atgccggcca cgtactcgag gtagtcgatg acacccatgt cctcgtagag
      661 gggggcgttt tccgggaggt atccgaggcg gcggcgcacc tcgagcgact gcgtggcgac
      721 atcgaagccg gccacctcca cctgcccgtc atcggcgggg agatagctcg tgaggatgcg
      781 catggtggtc gtcttgccgg cgccgttggg ccccaagaag cccagcacct cgccgcgggc
      841 caccgagaac gagatgtccc ggacggccac cgtcgacccg tatcgtttgg tcagtcggtc
      901 aacctggatc atggaagcgc ggaatttgaa cggagaaaat tatcgttgtc aacggggatc
      961 gcggcgggat ggaaccacgg agtcggggtc cggtttccac gactcccgcg ccggctggta
     1021 aactccgcgg tcgcgcttcc ggcgcatggt ttccccacca cggtcctcga ctgaagggct
     1081 ctcaatgaca gccataaacc gttttgctgc aattcaatac agcgcacttg ccatgcttgc
     1141 gctagccttc gcgagcccgg caaccgccaa gggccccgcc cggcagacgg cccctccgcc
     1201 accgctcgtg accggcccca cctatgagtg ggctgatttc gactcgctgc tggaccgtta
     1261 cgtggtgggt ggccgcgtga actacgccgc gtggaaggcg gacgggtcgg ccggactcga
     1321 caaggtcctg gcggccgcag gtgcctgggc catgtacgac agcgccacgt ccgaggagaa
     1381 agtggtgttc ctgtcgaatg cctacaacgg gttcgtcatc cagggagtgc tggccgccta
     1441 tccggtcaag agcgtgaagg acatccccgg tttcttcgac aaggccacac acgacatcgc
     1501 cggcggtcat tacacgctcg acgccctcga gaagacgctg ttgaaacccc tcgggcgcaa
     1561 gctgaacgac ccctactatc acatggccct ggtgtgcggc agcgtcggct gtccggcgct
     1621 ccgcaatcgt gcctatcgac aggccacctg gcagggggag acgatgaacc aggcccagca
     1681 cttcgtggcc gacccgacga agctccagtt cgaggaggag tcgaacaccc tgcgcgtcag
     1741 cgaactgttc gactggtaca tcagcgattt cgagagcggc gataccacgc tcccccgctg
     1801 gatcggcccg catctctccc tgggggcggc catgaagatg agcgccaccg agccgatcgt
     1861 cgaatacctg ccgttcgact gggcgctcaa cgacgttccg gcaggttcga agtgacgcgc
     1921 gtcactaccg cgccacggac gggtgaggtg ctgctcggcg agatcggctg ggcgcgcttc
     1981 aaggccggac acccctggat ctatcgcggc cagttcaagt cgccggccgc cgacctggcc
     2041 gccgggacag tgcgcatcgt cgatccgcgg ggaaaggtgc tgggaactgc actctacagc
     2101 ccggcctccc agatcgcgct tcgcctcctc acccgcgagg agacgacgat cgacgccgcc
     2161 tggttcgcct cacgcttcag cgcggcgcgc gactaccgcg accgcgtgat ggagccgcgc
     2221 tcggcgcgac gcgagttctt ctccgagtcg gacggcatcc cctccctcgt ggtcgatcgg
     2281 tatggcgatc atctcgtggt ccaggtcctg tcggccggcc tcgaagcgct ggagggcgcg
     2341 ctgttcgagg cgctgggcga ggtgtacaag ccggccagca tcctggcccg aaacgaggtc
     2401 agcgtccgga agctcgaggg gttggagcgc ggcgtgttcc agaagcacgg tgtcacgccg
     2461 gagcggatcg agatccagga aggtgagcgt acgctcgaag tcgacccgtg gagggggcag
     2521 aagaccggtc ttttcctcga ccagttcgag aaccacgccg cggccacgca actgacgagg
     2581 gggcgcgtcc tcgacgcctt tgcctccacc ggcggcttcg gccttgccat gtccggccgg
     2641 gcccgcgagg tggtggcgct cgacagctcg ggcgattcgc tggccacggc ggcccgcaac
     2701 gtgacgcgaa acggcctcga caacttcaag ttcgtggagg ccaacgcctt cgactggctc
     2761 aagtcggccg accaggccgg cgagaagttc gacgtcatcg tcctcgaccc gcccgccttc
     2821 gccaagaaca aggctgaagt cgaggcgggc gtgcgcggct acaaggagat caacctccgg
     2881 gcgatgaaga tgctcacgcc ggggggcgtg ctcgtgtcgt gttcctgttc gcaccacatc
     2941 acggaagagc tcttccacga cgtgctggcc gccgcggccg ccgacgtccg gcgccgtttc
     3001 cggatcatcg agaagcggac gcaggggcgc gaccatccca ttctcgtcgg ctttcccgag
     3061 agttactatc tgaagtgctt cctgctgcag ttcctggact gacccgccac ggccgaggtg
     3121 ccattcgatg accgaccgcg attcgctgat ccacgactgg aacctcgccg acggtccgcc
     3181 accccggcca tcccatgcca tcgagttcga tgacgagacg ctccgcgacg gcctgcaatc
     3241 gccgtcggtc accgatcctc cgatcgagaa aaagatcgag atcctgcacc tcatggaacg
     3301 gctcggcatc cagaccgccg acatcggcct gccggcggcc ggtccgcgcg cgcgggccga
     3361 cgtgaaacgc ctcgcggagg agatcgtccg gagcaagctc aggatcggcg ccaactgcgc
     3421 cgcccgcacc aagccggagg acatcgaccc cattgccgac gtgagccagg cggtcggcat
     3481 cccgatcgag gcctgcacct tcctcggctc gtcccccatc cgcatgtaca ccgaggggtg
     3541 gacggtggac cagctgctcc agctcaccga aaccgccgtg cgctacggcg tgagcaaggg
     3601 tctgccggtg atgatggtca ccgaggattc cacgcgcgcc catccggaaa cgctccggcg
     3661 catctacacc accgccatcg agtgcggtgc aaagcgggtg tgcgtggccg acaccaccgg
     3721 gcacgccacg ccggaaggga ccgccgccct gattcgtttc atcctgggcg tggtggccga
     3781 caccggcgaa gacgtgaagg tggactggca cggccacaat gaccgcggcc tcgggaccgt
     3841 caactgtatc gccgcggcgg ccgcgggtgc gcaccgcatc catggcacgg cgctcggcgt
     3901 cggagagcgg gtggggaacg cggcgctcga ccaggtgctg gtcaacttca agctcctcgg
     3961 ctggatcgac aacgacctct ccgcgctcgg ccagtactgc gagaccgtga gccgttccac
     4021 cgacgtgccg atcccggtca gctaccccgt cgtcggcagg gacgccttcc ggacggccac
     4081 cggcgtgcac gccgccgccg tcatcaaggc cgcccgcaag ggagacacct ggctcaccga
     4141 cgccatctat tccggcgtgc cggccggcat gttcggcctg gagcaggtca tcgagatcgg
     4201 gccgatgagc ggcgcctcca acgtgtcgtt ctggctcgag aagcgtggct atccggccaa
     4261 cgatgagttg gtgcaggcta tcttcggcga agccaagtcg gcggcccgcg tgctcaccga
     4321 cgcggaagtg cacgccatct gtcggacaaa gggcgtcaag gccggcgcgt gagtcgcccg
     4381 cagggcgatc ggtcgcggct ctacgccgcc gcctttctcc gctcgctggc caccgggctc
     4441 tcgggtgtgc tgctcgggct ctacctggcg cgcgtcgggc tcagcgccac caccatcggc
     4501 tacgcgatca gcgccggcct gggcggcggc gcgctcgccg ccaccatcac cgcgttcttc
     4561 ggcgatcgga tcggccggcg gcgcctcctg gtggcggtca atctccttgg agcgatcggc
     4621 gggcttgtcc tctacttcgg cgattcgccg tggctgttga tcgccgcatc gttcgccggg
     4681 atggtgaacg gcatggggcg cgatcggggc gccacgctga tcctcgacca ggccatcctt
     4741 ccgggcacca ccgacaacac cggccggacg aaagtgtttg cggtgtacaa cgtattccag
     4801 gacatcggcc acgcggtcgg cggtttgctg gcggccattc ccgacatgag atcggccttc
     4861 ctcgtctact cgggcctcct ccttgccgcg gcaatcctcg acatgacgct gtcgagtgcc
     4921 gtcgaggcgg ccgaaccgac ccgacgaccc actctctccc cgaaatcgcg gtcactcatc
     4981 acgcgcatct ccgccctctt cgccatggac agcctcggag gcggattcct caccacgagc
     5041 cttctcgcct atttctttca tgagcgcttc ggagtttctg ccgggacgat cgggttcctc
     5101 ttcttcgccg cccgcgtggc caatgccggg agccacctgg ccgccgcgtg gctggcgacg
     5161 cgtatcggcc tcgtcaacac gatggtgttc acccaccttc cttccagcct gctgctgctg
     5221 accgtgccgt tcgcgccgaa cttcaccgtg gccgccatcc tttttctcct gcgcgaatcg
     5281 ctcgtcgaga tggacgtgcc gacgcggcag tcgtacgtca tggccgtggt ggcgccggag
     5341 gaacggaccg tggcttcggg catcacgcac ctcgtgcggc tcacggcctg ggcggtggcg
     5401 ccggccttcg cgggcgccat gatgacgggt gcgctcggcg cgccgctggc ggccggggcg
     5461 gggatgaaga tcgcgtacga cctgttgttg tggcgggcat tccggaaggt gcggccgccg
     5521 gaggaacatc ccgcctgatc gggcggggaa tcatggacgc tcaggcccga ccggccacgc
     5581 cccagaacgt ctcgccatcc acccactggc ggtaggcctc gttgagctcg gccaccaggt
     5641 attcgagatg ctcttcggcg cctgagagcc aggcggcttc ctccagcgag tcgacgaagc
     5701 gaccggggca gccggaacag ccggggtgct cggggcagcc ggccagaaag tgccggtcga
     5761 ggatagccgc ggcacccggg aagaggttca ggatatcgcc gaccagccag ttgggcgtga
     5821 tctcggcata gcgatggccc gcctcggcat cgaacgggat ggggtgatca accatgcccc
     5881 tgattttagc aggtggggcc gggagggttg tggcgacccg acgcggttgc tagcctcgct
     5941 cgatgcgaaa ttcaaactgg attcgatggg gtcttggtgc ggcacttctc ctgaccggtg
     6001 ggtgcggccg caagtcgaac accgagtggg ttcgccccgc ccccacgccg ctgaccacgg
     6061 atgcccgatt cgatggtgaa ccctccttgt cgcccgacgg tcgccaggtg gtcttcgtct
     6121 cgttcggtga atccgatctc gatctcttcg tgatcgaccc cgccaggcca tcggagcggc
     6181 gcctcctcca tggcggcccg ggcgacgacc gacagccggc ctacagtcca gatggcaagc
     6241 gcctggcctg gacgctggtg accggtgacg attcggacat catgatgacg ggcgaagggg
     6301 gtgatgcccg ccgggtgacc gtggaagccg gccatgatca gcagccggcc tggagcgccg
     6361 acggtacccg cctcgccttc gtgagcgatc gaaccggccg caccgaaatc tgggtgacga
     6421 cgctgtcgac cgggcaatcg gccccgatct cggccgcttc cccgccgtcg aacgactacg
     6481 tgctggccga ccccgcctgg cggggcgaag cggtcgtggt gtcggcaacc gtggatgacc
     6541 aggtcgatct atgggagatc gcggccacgg gcggcgcctg gagacgcctg acggcgggac
     6601 cggcccgcga acggcacccc gatcccggcc ccggcgaccg gctggctttc acctccgaca
     6661 cgaccggcta cgccaacctc tacgtgcgcg aaccggacgg ccggatcacc gcgctcaccg
     6721 acgagcgcac cgacatcttc gaatcgcgct ggacatccga cggaagccgg atcctct
//
LOCUS       DASVOD010000551         1823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_609134, whole genome shotgun sequence.
ACCESSION   DASVOD010000551 DASVOD010000000
VERSION     DASVOD010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609134"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VF720_12215"
     CDS             <1..686
                     /locus_tag="VF720_12215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7880170.1"
                     /translation="LLMHLLADGTIDSTWPVGGRSMGSMPIGSQSWPGPRMVADGSGG
                     TIIAWTGRGWDHIYAQRVLSDGTLDPAWPPATGTTVSAGPNKYDPMSVPDGSGGAIIT
                     WYESRPGGNGLDICAQRVRSDGRLGEEVSGVQDVAPFGLVLQPIRPNPLRTETFAVPF
                     RVGGDGAVSIEVFDVAGRRITSRQLASVEPGFHSIEVSLGPSPVAGIYFVRLQQGTLV
                     RTTTVTLLD"
     gene            837..1661
                     /locus_tag="VF720_12220"
     CDS             837..1661
                     /locus_tag="VF720_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880171.1"
                     /translation="MQLAIRNPLIRSLLSTAALAVLAGLPATSDAGSLFLTGHDPDFH
                     ATLGGNTAGAIKINQVAIGFVQDPAFNPFYAGTPKFLFVESRIAPPGGHTVGKAGIVA
                     SGYVEGVNFDHADATQLNAALNNLGTVYSAIVVASDFGGLLTQAELNILNSRNADIIA
                     FLNAGGGIYCLAEGNGGVGLTPGGGWLAFLPIAAVSPAIDQSEVGNTVTAYGAGLGLT
                     NADVNGNASHTIFTAYGTLSPVDLDAAGRVLTVAGHDAIIAVESSTWSRIKKLAGN"
BASE COUNT          325 a          628 c          551 g          319 t
ORIGIN      
        1 tgctcctaat gcacctcctg gccgatggca cgatcgactc gacatggccc gtaggtggac
       61 gatcaatggg gagcatgccg atcggctcgc aatcatggcc tggaccccgg atggtcgcgg
      121 acggttcggg tgggacgatc atcgcctgga caggtcgtgg gtgggaccac atctacgcac
      181 aacgcgtcct gtccgacggc acgctcgacc ctgcgtggcc ccctgctacc ggcaccaccg
      241 tcagcgccgg acccaacaag tacgatccga tgagtgtccc ggatggcagt ggcggcgcga
      301 tcatcacctg gtatgaaagc cgtcccgggg ggaatggcct cgacatctgc gcgcaacgcg
      361 tgcgaagcga cggtcgactc ggggaagagg tttccggggt gcaggatgtt gcgccgttcg
      421 gattggttct ccaaccgata cgtcccaatc cgcttcggac ggaaacgttc gccgtgccgt
      481 tcagggtggg aggcgatggg gcggtgtcca tcgaggtgtt cgatgtcgcg ggacggcgga
      541 taacctcgcg gcaactggct tcggtggaac cgggcttcca ttcgatcgaa gtgtccctcg
      601 gcccgagtcc agtggcggga atttacttcg tccgactcca gcagggcact ctcgtgagga
      661 cgacaacggt caccctgttg gactgagcct caacgcgtgt tgcaacagca acaactcgcg
      721 tgatgacccc gccggccaca tcacgaaaac ccgaaaattc cgggatcaag ccaccggccc
      781 ctgttctatg ctccaccccg ttcgtcgccc ttgtccaact cacccggagg tccctcatgc
      841 aactggcgat ccgtaatccg ctcattcgtt ccctcctctc aacggccgcg ttggccgtac
      901 tggccggact ccccgcgacg tccgatgccg gctcgctctt cctgaccggc cacgaccccg
      961 acttccacgc cacgctcggc ggcaataccg ccggcgccat caagatcaac caggtggcca
     1021 tcggcttcgt gcaggatccg gcattcaatc cgttctacgc gggcaccccc aagttcctgt
     1081 tcgtcgagtc gcgcatcgct ccgccgggcg gccacacggt cggcaaggcg ggcatcgtgg
     1141 ccagcggtta cgtcgagggc gtgaacttcg accacgccga cgcgacgcag ctcaacgccg
     1201 cactgaacaa tctcggcacg gtctacagtg ccatcgtcgt ggcctccgac ttcggcggtc
     1261 tcctgaccca ggccgagctc aacatcctca actcgcgcaa cgccgacatc atcgccttcc
     1321 tgaacgccgg cggcggcatc tactgcctgg ccgaaggaaa cggcggcgtc ggcctcacgc
     1381 cgggcggcgg ctggttggcc ttcctgccga tcgcggccgt ctcgcccgcc atcgaccagt
     1441 ccgaagtcgg caacaccgtg accgcctacg gcgccggcct cggcctcacc aacgccgacg
     1501 tcaacggcaa cgcctcgcac acgatcttca ccgcctacgg gacgctgtcg ccggtcgacc
     1561 tcgacgcggc cggacgcgtg ctgacggtgg cggggcatga cgcgatcatc gcggtcgagt
     1621 cgtcgacctg gtcgcgtatc aagaagctgg cggggaactg acagccgggc gatcgaaggt
     1681 cggatagatc agcgcaatag aaaaacgtcg ggccgggagc tccattgaac tcccggcccg
     1741 acgatgtcta actacaagag tgtttcgaga aatccttcgg atttctcgat ccttctagtg
     1801 cccgtgccca tgatgcccgc cac
//
LOCUS       DASVOD010000552         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_870538, whole genome shotgun sequence.
ACCESSION   DASVOD010000552 DASVOD010000000
VERSION     DASVOD010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870538"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1703
                     /locus_tag="VF720_12225"
     CDS             <1..1703
                     /locus_tag="VF720_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880172.1"
                     /translation="TETWFDRGIEECRVSNGRTIVAGDFWMVDGKTARFLAVDSGTGF
                     AFPTSPNGPRGGADMPVRALLPYRDRLVVGGSFVALSGQSHPFLAIGDGRHWDSPGGG
                     VDNGVFALTEFEGGVVAAGDFTTAGGRPASRIALWDGAEWHPLGDGFDDRVMALVVHE
                     GRLYAGGWFRHSGSRSIRHLAVWDGTNWASAGDIDGSVNALASFRGVLYAGGLIRNGD
                     GVAASSLVRFDGATWEPVADLKGIVLAMASNGHELAIAGQLRGAPWVGGPAVVLWNGS
                     DALPLPWQDTRLPSISAVAWTGRGLVVGGNFEHVKSPHLRNLARWNGSDWESFGTGID
                     RSVLAVADVNGSLFVGGNFESAGDRLSERLARWDDGAVPLVVDGVTVDRDESRVRLSW
                     NGSGVSNVRRTEVWVGPAESWMSGPAEGGSGTFVGTATDIGNGHFEFVDDSARADGTS
                     YWFRQLGPFEDDSWSGPWALAPLPAGLRLLSIGPNPFLGSAEIHYSTAHAGPLRIDVF
                     DAQGRRVRQLADGEHPAGIFTLAWDGRAESGSPVAAGTYWVRLATRDGIQSRRILRLR
                     "
BASE COUNT          255 a          531 c          645 g          347 t
ORIGIN      
        1 cgaccgaaac ctggttcgat cggggaatcg aagaatgtcg tgtgtcgaac gggcgcacga
       61 tcgtggcggg cgacttctgg atggtggatg gtaagacggc gcggttcctg gcagtcgatt
      121 ccggtaccgg cttcgcgttt cccacgtccc cgaacgggcc gcgcggcgga gccgacatgc
      181 cggtgcgcgc gctacttccg tatcgcgacc gtctcgtggt gggcgggagc ttcgtggcat
      241 tgagtggcca atctcatcct ttcctggcta tcggggatgg acgccactgg gacagcccgg
      301 gaggcggcgt cgacaacggc gtcttcgcgt tgacggaatt tgaaggcggg gtggtcgccg
      361 cgggcgattt cacgacggcg ggcggccgac cggcatcgcg catcgcgcta tgggatggag
      421 cggaatggca tcccctcggc gatgggtttg acgatcgcgt gatggcactc gtcgtccacg
      481 agggacgact ctacgccgga gggtggttcc gtcattccgg atcacgttcg atccgtcacc
      541 tcgccgtctg ggacggcacg aactgggcat cggcagggga catcgacggt agcgtgaacg
      601 ctctggccag tttccgagga gtgctttacg ccggcggtct cattcggaac ggcgatggcg
      661 tcgcggcgtc gtccctggtg cggttcgacg gtgcgacatg ggagccggtg gcggacttga
      721 aaggcattgt cctcgccatg gcctccaatg gtcacgaatt ggccattgcc ggccagcttc
      781 gaggggctcc gtgggtcggt gggccggccg tcgtcctctg gaacggctcc gatgctctgc
      841 cgttgccgtg gcaggacacc cggttgccct cgatttcagc cgtggcctgg accggccggg
      901 ggctcgtcgt cgggggcaac ttcgagcatg tgaagtcacc tcatctgcga aacctggctc
      961 gctggaatgg cagcgattgg gagtcgttcg gcaccgggat cgaccgctcg gtcctggcgg
     1021 tcgccgatgt gaatggaagc ctgttcgtgg ggggcaattt cgaatccgcc ggcgatcggc
     1081 tgtcagagcg actggcgcgc tgggatgatg gcgccgtgcc cctcgtcgtc gacggcgtga
     1141 ccgtggaccg tgacgaatcg cgcgtccggc tcagctggaa cggatccggt gtaagcaatg
     1201 tccgtagaac ggaagtgtgg gtcggcccgg ccgagtcgtg gatgagcggt ccggcggagg
     1261 gcggttccgg aacgtttgtc ggaacggcga cggacatcgg aaacgggcac tttgagttcg
     1321 tggacgactc cgcccgcgcc gacggaacgt cctactggtt ccgccagttg ggtccattcg
     1381 aggacgactc ctggtccggg ccgtgggcgc tggcgccgct tccggccggc ctgcgactcc
     1441 tgtcgatcgg accgaacccg ttcctgggtt cggccgaaat ccattactcg acggcgcacg
     1501 ctggcccgct tcggatcgac gtgttcgatg cccagggacg gcgtgtgcgg cagctggcgg
     1561 acggcgaaca tccggcaggc atcttcacgc tcgcctggga cgggcgcgcc gagtcgggtt
     1621 cgcccgtggc cgccggcacc tactgggtac gcctcgccac ccgtgacggc attcagtccc
     1681 gtcgcatcct gcgcctgcgc tagttgtgat ttctcaatag gttgttcact gcccgggggg
     1741 agtagtcacc cggggctctt ctgttggaag ttggtggt
//
LOCUS       DASVOD010000553         2441 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_976250, whole genome shotgun sequence.
ACCESSION   DASVOD010000553 DASVOD010000000
VERSION     DASVOD010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976250"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="VF720_12230"
     CDS             <1..1169
                     /locus_tag="VF720_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7880173.1"
                     /translation="AGFEIRVRDSGMGIPPEEQERIFDRFHQGDTAIKSQPGTGIGLS
                     LARELVELHKGSIVVESQVGQGTTFLVRLPAGREHLRPEDMADGEVVGPHGAPASTSM
                     HDPFDADDPVDHAADESATADRMSVLIVEDHPQVRALVRKHLEPRYRILEAEDGRKGL
                     AMALREIPDLIVSDVMMPGMDGMELTKAVKDSAEIGFVPVLLLTARADLEDRLTAYSR
                     GADAYLAKPFEPRELVARIDGLIQERRRLKERYAALAPGLHLAGMTSSDEAYLTSVSQ
                     VIMKNLADEDFSVEALARQLGQTRTTLFRRIKEAGHTTPSAMIRRLRLEQGRLMLEKG
                     VGSVNEVAYGVGFKSVSHFCTAFRLAYGSAPGAWKAKGADPLDKPDRTRGTIDL"
     gene            1390..>2441
                     /locus_tag="VF720_12235"
     CDS             1390..>2441
                     /locus_tag="VF720_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HEX7880174.1"
                     /translation="MIVGFAASNARAQATILSGNTSTSTAGTSHQQGISDNPNASLSG
                     LISQDPFGNEASANGTLAALDGRFKVRAHACSGGGPGSGGSANAIAQCQVTLDYKVVS
                     TTLPAGTPVTIRINWAVSGRSTAAGNLTHYISGAIGTLNGHVSITVGGVSIINRSGER
                     RHEYNLNSGYTHPVFGTLNPEADSLTHVVTALVGQNVRIFMSGSAAGNSVATLSSVTD
                     GDAQLAMVWGISSMDPNASVESVNYMMNPAPPASNATLPMADDYKDPRPPGLQQCFEF
                     SSQPGPVSVCASSNTGFSVSATGTGPFTYRWYRNGVPVDAIANPSATTANLVLNGVNS
                     GQGGNYTVKVTNPCGDR"
BASE COUNT          431 a          837 c          770 g          403 t
ORIGIN      
        1 atgccggctt cgagatccgc gtccgcgact ccgggatggg catccccccg gaagagcagg
       61 agcgcatctt cgatcgcttc catcagggcg acacggccat caagtcgcag ccgggcaccg
      121 gcatcgggct atcgctggcc cgcgagctgg ttgaactgca caaggggtcg attgtcgtcg
      181 agagccaggt cggccaggga acgacgttcc tcgtgcggct cccggcgggt cgcgaacatc
      241 tccgcccgga ggacatggcc gacggtgaag ttgtcggacc gcacggcgcg ccggcatcca
      301 cttcgatgca cgacccgttc gacgccgacg atcccgtcga ccacgccgcc gacgaatcgg
      361 ccaccgccga ccgcatgagc gtgctcatcg tcgaggacca tccccaggtg cgggccctgg
      421 tccgcaagca cctggagccc cgctaccgga tcctcgaagc cgaggacggc cggaaggggt
      481 tggccatggc cctgcgcgag atccccgacc tcatcgtctc cgacgtcatg atgccgggta
      541 tggacggcat ggagctcacg aaggccgtga aggacagcgc cgagatcggg ttcgtcccgg
      601 tcctgctcct gaccgcccgc gccgatctgg aggaccgcct caccgcctat tcgcgcggcg
      661 cggacgccta cctggccaag ccgttcgagc cgcgcgaact ggtggcgcgc atcgacggcc
      721 tcatccagga gcgccgccgc ctcaaggagc gctacgccgc cctggctccc ggcctccatc
      781 tggccggcat gacctcatcc gacgaggcgt acctgacgtc agtttctcag gtcatcatga
      841 agaacctggc cgacgaagac ttcagcgtgg aagccctcgc gaggcaattg ggccagaccc
      901 gcacgacgct cttccgccgg atcaaggagg cgggccacac gacgccctcc gccatgatcc
      961 gccgactccg cctggagcag gggcgcctca tgctcgaaaa gggggtcggc tcggtgaacg
     1021 aagttgccta cggcgtgggg ttcaagagcg tgtcccactt ctgcacggcg ttccgcctgg
     1081 cctacggatc ggcgccgggc gcgtggaagg caaagggggc ggatcccctc gacaagcccg
     1141 accggacgcg cggaacgatc gacctctgac aacaagtatt gattgggccg acgtatggat
     1201 tccgggaatt tcattgctcg cgccgttatc ccgctggtag tatcgatgct catccgcatt
     1261 gaccgacgct cgttgcccaa tcattcaaag actttgcaag ggggttgtct catgaattgc
     1321 gtggccgccg tccgtcgtgc tcagcgcctc ccggcgcttt ttttgtgggc tctcccggca
     1381 ctggccctca tgattgtcgg attcgccgct tcgaacgcgc gggcccaggc caccatcctc
     1441 agcgggaaca ccagcacgag cacggcgggg acgtcccacc agcaggggat ctcggacaat
     1501 ccgaacgcat ccctgagcgg gctgatctcg caggatccct tcgggaacga ggcttcggcc
     1561 aatggaacgc tcgcggcact ggacggccgg ttcaaggtga gggcacacgc ctgttccggt
     1621 ggcgggcccg gctctggagg gagcgccaac gccatcgccc agtgccaggt gacgctggac
     1681 tacaaggtcg tctcgacgac gctccctgcc ggcaccccgg tcacgatcag gatcaactgg
     1741 gcggtcagcg gtcgaagcac ggccgccggc aacctgacgc actacatttc cggggcgata
     1801 ggcaccttga acggccacgt gtccatcacg gtgggcggag tcagcatcat caaccggtcg
     1861 ggtgagcgcc ggcatgaata caacctgaac agcggctaca cgcaccccgt tttcggaacc
     1921 ctcaatcccg aggcggacag cctgacgcac gtcgtcaccg ccctggtcgg gcagaacgtc
     1981 cggattttca tgtcgggatc ggctgccgga aattccgtcg ccaccttgag ctcggtcacc
     2041 gatggcgacg cgcagctggc catggtatgg ggcatcagct cgatggaccc gaatgcgtcg
     2101 gtggaatcgg tcaattacat gatgaatcct gcgccgccgg cctcgaatgc cacgctcccg
     2161 atggccgacg attacaagga cccgcgtccc cccggcctgc agcagtgctt cgagttctcc
     2221 agccagccgg gcccggtcag cgtttgcgcc tcgtcgaaca ccggtttttc ggtttcggcc
     2281 accggcacgg gccctttcac ctacaggtgg tatcgcaacg gcgtgccggt ggacgccatc
     2341 gcaaacccct cggccacgac cgcgaatctt gtgctgaacg gcgtcaactc cggccagggc
     2401 ggtaactaca cggtcaaggt caccaatccc tgcggcgatc g
//
LOCUS       DASVOD010000554         7428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_38537, whole genome shotgun sequence.
ACCESSION   DASVOD010000554 DASVOD010000000
VERSION     DASVOD010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7428
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38537"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..111)
                     /locus_tag="VF720_12240"
     tRNA            complement(35..111)
                     /locus_tag="VF720_12240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(75..77),aa:Gly,seq:gcc)
     gene            203..1429
                     /locus_tag="VF720_12245"
     CDS             203..1429
                     /locus_tag="VF720_12245"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7880175.1"
                     /translation="MALQVHKFGGTSVGTPERIRSVAARLAALSAEGHQLVVVVSAMG
                     DTTDELLQLAAAISDNPHRREMDMLLSAGERISMALLSMALNALGCPAVSFTGSQSGI
                     ITDSGHGRARIVDIRPVRIREELEKGRVVIVAGFQGVSVEKEVTTLGRGGSDTSAVAL
                     AVAFGAAECHIWTDVDGVMSADPRLVPGARHLARLDFDTMLEFSSRGAGVINVRAVEL
                     GRKFRMPIVILNSLRERTGTRVEDLTDMERTGITGVTANDRLIGFHLSDLVLDMAGLA
                     DFLGRLIELGLDLSGLHEYVDGPGTCGLSFLAQDLPENRRGFDRIERMAAEAGGSVRR
                     DESVGAVSVVTSGVIQAGPLARRIVACLGGAGVTVRSMSSGTTSITLMIDRESIPGAV
                     AALHRELVEDEATLRG"
     gene            1523..3382
                     /locus_tag="VF720_12250"
     CDS             1523..3382
                     /locus_tag="VF720_12250"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEX7880176.1"
                     /translation="MSAPARPPIADVLGKKILVFDGAMGTMLYAEGVPFTQCFDAMNT
                     ARPDVVRLVHMAYVSAGADIIETNTFGGNRFRLAEHGLEKEVAAINRAGARLAREAAG
                     DRAWVAGSVGPIGRPLAPIGTIPPEEAEEAFREQIAALVEGGIDLLVIETIGDMNEME
                     RAVAAARSAAPGIPVIAQMTFSEEGKTLIGNKPAEIARRLSDLGVAAVGANCSVGPQG
                     ILDVIERMSSTSRVPLVAQPNAGLPHLVGGRLLYLATPEYFEEYARRLVEAGASLIGG
                     CCGTTPEHIAAIRRGTAGLALSSAARVNGGATIEVAEPEPTAPAAPAAERSTFGSKLG
                     RRFVISVEIDPPRGLDLEKYLHGALMLRQKGADAINIADSPLGRARMAPMAMGHLIER
                     SVGIESIIHVCCRDRNLLALQSELLGAHALGLRNLMLITGDPVLAGDALQATDVFDLD
                     SIGLIRLAAALNKGVDLAGKSVDRPTSFVIGCAANPTAVDIERDIDRLREKVEAGADY
                     VFTQPLYDPRDLETYLARVTDLKVPICVGVLPLRNHRHSEFLHHEVPGMRVPESIREE
                     MRKAGEQGAAAGVAIARRFLTEVQSMVAGAYLMPPFNRFDMAADVMEGFVEVD"
     gene            complement(3434..4528)
                     /locus_tag="VF720_12255"
     CDS             complement(3434..4528)
                     /locus_tag="VF720_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016116248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEX7880177.1"
                     /translation="MKIFERMARHEHEQFVLCNDTASGLKAIIAIHDTTLGPALGGCR
                     MWPYASEDEALEDVARLSRGMTYKAAAAGLNLGGGKSVLIGDPKKDKSEAMFRAFGRF
                     IQGLSGRYITAEDVGTSVPDMVHVRTETRFVTGIPSTLGGSGDPSPFTAMGVYRGMKA
                     CAKKVFGSDDLSGRSVAIQGMGNTGWYLATYLKAEGARLLVADIDADRVKRAVTELGA
                     EGVGADAIHSANVDIFSPAALGGIINDATIPTLRARIVAGSANNQLADEEKHGAELKS
                     RGILYAPDFVINAGGLINVANELVGYNKELATQQVNAIGDIIARIIELAEADKVSTHV
                     AANRLAEDRIARIAATRHIRVRGHTADPRG"
     gene            4668..5549
                     /locus_tag="VF720_12260"
     CDS             4668..5549
                     /locus_tag="VF720_12260"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX7880178.1"
                     /translation="MLTGAHMSIAGGVDTSVARGIEVGCRTMQIFTKNANQWTAAPIH
                     ADTARRFREELDRSGIGPVVSHDSYLINLGSPDLALREKSMAAFVDELDRAEQLGLVG
                     VVTHPGAHMGQGEEAGLERVAASLRAVLKTTHRHKVAILIENTAGQGTSLGWAVSHLA
                     TLLAGVEGHSRIGFCLDTCHLHAAGHDLSTDASYGAFIEAFDAAVGLDRLRCFHMNDS
                     LKPFGSRVDRHTHIGQGTIGEEPFRRIMCDTRLAHVPKILETPKVGSGVTEDRMNLAT
                     LARLGTPPAPARKRKAG"
     gene            5546..6058
                     /locus_tag="VF720_12265"
     CDS             5546..6058
                     /locus_tag="VF720_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEX7880179.1"
                     /translation="MTLADGLILFLLIVSAGMGLARGFVREITGLVGLLAAIAFGALL
                     GPGLAGSVFGWIPPPFAGIGAFLTVFLAVLFISALVGRLLTAAAHAASLSFPNRVLGG
                     LFGLVRAACFLLALLFALDLVGVDAGPWLKGSRLGEPAWQAVQRLREATGAHPMDAPV
                     KPGPPGSTTI"
     gene            complement(6152..6853)
                     /locus_tag="VF720_12270"
     CDS             complement(6152..6853)
                     /locus_tag="VF720_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880180.1"
                     /translation="MRHKGTASLIALFAAVVLSGTASAATNPVIDWTGPESAAWEPNF
                     VAPHNSVAGNVLTIVGKITLPYNSPLTSLNPVDGATEYTFVFTGMTSQGTVDLGPVRR
                     TDYTGGAWTIYADGSPDRAFGTNPPNATSPSTFNDGSVVLSGTFNNFYTVSNELNNGG
                     NYNADISVTGGSAYGLMQTDNALCGYLIGVWNRTPGSFPTGYIRSADGKMDFFGCPVP
                     VAPSTWSRIKGLLSE"
     gene            complement(6933..>7428)
                     /locus_tag="VF720_12275"
     CDS             complement(6933..>7428)
                     /locus_tag="VF720_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880181.1"
                     /translation="YTFTLGEDLFFASADTFGTIVVASYVNGEINFYEDSRTTGTLGT
                     FNLLGECDPFFDRLTYNDGTLILHGDFASFDVVFSTTTGDGNLAGLTNWTGGSQLGNI
                     PVGQRSGWTFGAIGIRAGNTPCGYHWQIDGECYLQDPLAVTPSTWGQIKSLDIPNSNI
                     RIHR"
BASE COUNT         1167 a         2459 c         2544 g         1258 t
ORIGIN      
        1 gtaacggagt cctggcggac cccggggatg tgaatggagc ggaagacggg attcgaaccc
       61 gcgacaacca ccttggcaag gtggagctct accgggctga gctacttccg ctcgtaacgg
      121 cttcgacagc ttttgcgccg aaaccggtgg aggaagatat tcgactgccc gcggtcgtgt
      181 caagcggata tcatccgtcg tcatggcgct ccaggtacac aagttcggcg ggacatcggt
      241 ggggacgccg gagcgcatcc ggtcggtggc ggcccgtctt gccgccctgt cggccgaggg
      301 acatcagctc gtcgtggtgg tttccgccat gggcgacacg acggacgagc tcctccagct
      361 cgccgcggcc atctccgaca acccccatcg ccgtgaaatg gacatgcttc tctcggcggg
      421 cgaacggatc tcgatggccc tcctctcgat ggcactgaat gcgctcggat gcccggcggt
      481 gtcattcacg gggagccaga gcggcatcat caccgattcc gggcacggcc gggcgcgcat
      541 cgtcgatatc cgtccggtgc ggatccgcga agagctcgaa aagggacgcg tggtcattgt
      601 ggccggcttc cagggcgtga gcgtcgagaa ggaagtgacg acgctggggc gcggcgggtc
      661 cgacacttcg gcggtggctc tggcggtggc gttcggcgcc gccgagtgcc acatctggac
      721 cgacgtggac ggcgtcatga gcgccgatcc gcgcctggta cccggggcgc gacacctggc
      781 ccgcctcgac ttcgatacga tgctcgagtt ctccagccgg ggcgccggcg tgatcaacgt
      841 ccgcgcggtc gagctgggac gcaagttccg gatgccgatc gtcatcctca acagcctgcg
      901 ggagcgcacg ggcacccgcg tcgaggacct caccgacatg gaacgaacgg gaatcaccgg
      961 cgtcaccgcc aacgaccgtc tcatcggatt ccacctcagc gacctcgtgc tcgacatggc
     1021 gggcctggcc gacttccttg ggcggctcat cgagctgggg ctcgacctct cgggcctcca
     1081 cgagtacgtg gacgggcccg gtacctgcgg cctgtcgttc ctggcccagg atcttcccga
     1141 gaatcgacgc ggcttcgatc ggatcgaacg gatggcggcc gaggcgggcg gaagcgtccg
     1201 gcgcgatgaa tccgtgggcg ccgtttccgt tgtcaccagc ggtgtcatcc aggccggccc
     1261 gctggcccgc cgcatcgtgg cctgcctcgg cggcgcgggc gtgacggtgc gctcgatgag
     1321 ttcggggacg acctcgatca cgctcatgat cgatcgggag tcgattcccg gcgccgtggc
     1381 cgcccttcac cgggaactcg tcgaggacga ggccacgctc cgcggctgat cgcccgtccc
     1441 gacgtcgggg gccgggtgtc gtatactttc aggtccgatt tgggcccccc tcgtggcaaa
     1501 ggagacgttt cgcttgaatc ccgtgtccgc ccccgctcgc ccccccatcg ccgatgtcct
     1561 cggaaagaag atcctcgttt tcgacggcgc gatgggaacg atgctctacg cggagggcgt
     1621 cccattcacg caatgcttcg acgccatgaa cacggcccgg cccgacgtgg tgcggctggt
     1681 gcacatggcc tacgtgtcgg ccggcgcgga catcatcgaa accaacacgt tcggcggcaa
     1741 ccgcttccgc ctggccgaac acggcctcga gaaggaggtg gcggccatca accgggccgg
     1801 cgcccggctg gcccgcgagg cggccggcga cagggcctgg gtggccggat ccgtcgggcc
     1861 catcgggagg ccactggctc ccatcggcac cattccgccc gaggaagccg aggaagcctt
     1921 tcgggagcag atcgccgcgc tcgtcgaggg aggaatcgac ctgctggtca tcgagacgat
     1981 cggcgacatg aacgagatgg aacgtgccgt ggccgcggcc cggagcgcgg cgccgggcat
     2041 cccggtcatc gcccagatga ccttttccga ggaggggaag acgctcatcg gcaacaagcc
     2101 ggccgagatc gcccgccgcc tgtcggacct cggcgtggcc gccgtgggcg ccaactgctc
     2161 ggtggggccc caagggatcc tcgacgtcat cgaaaggatg tcgtcaactt cgcgcgtgcc
     2221 gctggtggcg caaccgaacg ccggtctacc ccacctcgtg ggcgggcgcc tgctctacct
     2281 ggccacgccg gaatacttcg aggagtacgc ccgccgcctg gtcgaggcgg gcgccagcct
     2341 catcggcgga tgctgcggca cgacgcccga gcacatcgcc gccatccgac gcggaacggc
     2401 gggactcgcg ctctcgagcg cggcccgtgt caatggcggc gcgacgatcg aggtggccga
     2461 accggagccg acggctccag ccgcaccggc cgccgagcgg agcacgttcg gttcgaagct
     2521 cggccgccgc ttcgtgatct cggtcgagat cgatccgccc cgcgggctcg acctggaaaa
     2581 gtacctgcat ggcgccctga tgctccgcca gaagggtgcc gacgccatca acatcgccga
     2641 cagtccgctc ggccgcgcgc ggatggcacc gatggccatg ggacacctca tcgagcgcag
     2701 cgtgggaatc gagtcgatca tccatgtgtg ctgccgcgat cgaaacctcc tcgcgctgca
     2761 gtcggaactg ctcggcgcgc acgcgctcgg cctgcggaac ctcatgctga tcaccggaga
     2821 cccggtgctg gcgggcgacg cgctgcaggc caccgatgtc ttcgacctgg actcgatcgg
     2881 cctgattcgc ctggccgccg cgctcaacaa gggggtggac ctggcgggca agtcggtgga
     2941 ccgaccgacc tcgttcgtga tcggctgcgc cgccaacccg accgccgtgg acatcgaacg
     3001 tgatatcgac cggctccgcg aaaaggtgga ggccggcgcc gattacgtgt tcacgcagcc
     3061 gctgtacgac ccacgcgacc tcgagaccta cctggcccgc gtgaccgacc tcaaggtgcc
     3121 catctgcgtg ggcgtgctgc cgctccgcaa ccaccgccac tcggaattcc tgcaccacga
     3181 agttccgggg atgcgggtgc ccgaatcgat ccgcgaggag atgcggaagg ccggcgaaca
     3241 gggcgccgcc gccggggtgg ccatcgcacg gcgcttcctg accgaggtgc agtcgatggt
     3301 tgccggcgct tacctgatgc cgccgttcaa ccgcttcgac atggcggcgg acgtgatgga
     3361 agggttcgtc gaggtcgact gacgggttcc ggctcccggg atcggtacag ccgccgaaac
     3421 cgggccggcc tgcctacccc cgtggatcgg cggtatgccc ccgcacccgg atgtggcgtg
     3481 tggccgcgat ccgggcgatg cgatcctcgg ccagccggtt ggccgccacg tgcgtggaca
     3541 ccttgtcggc ctcggccagc tcgatgatcc gcgcgatgat gtcgccgatg gcatttacct
     3601 gttgggtggc cagctccttg ttgtagccga ccagttcgtt ggccacgttg atgaggccgc
     3661 cggcgttgat cacgaagtcg ggcgcgtaga ggatgcctcg cgacttgagc tcggcgccat
     3721 gcttctcctc gtcggccagc tggttgttgg ccgagccggc cacgatcctg gccctgagtg
     3781 tcgggatcgt tgcgtcgttg atgatgccgc cgagagccgc ggggctgaag atgtcgacat
     3841 tggccgagtg gatggcatcc gccccgactc cttcggcgcc cagctcggtg acggcgcgct
     3901 tgacgcgatc ggcgtcgatg tcggccacga ggaggcgggc cccctcggcc ttgaggtacg
     3961 tggcgaggta ccaccccgtg ttccccattc cctggatggc caccgagcgg ccagacaggt
     4021 cgtcggaacc gaacactttc ttcgcgcagg ccttcatgcc ccgatagacg cccatggcgg
     4081 tgaacggcga gggatcgccg ctgccgccca gcgtgctcgg gatgccggtc acgaagcgcg
     4141 tctcggtgcg cacgtgcacc atgtcgggca cggaagtacc gacgtcctcg gcggtgatgt
     4201 accggccgga aagtccctgg atgaagcggc cgaaggcgcg gaacatggcc tccgacttgt
     4261 ccttcttcgg atcgccgatc aggaccgact tgccccctcc caggttcaac ccggcggccg
     4321 ccgccttgta ggtcatcccg cgggagaggc gggccacgtc ttccagcgct tcgtcctcgc
     4381 tggcgtacgg ccacatgcgg catccgccga gcgcgggtcc aagtgtcgtg tcgtgaatgg
     4441 cgatgatggc cttgagcccc gaggccgtgt cgttgcacag gacgaattgc tcgtgttcgt
     4501 gccgggccat gcgctcgaaa atcttcatcg gggatccctt cgggtggggg gggaggagcg
     4561 attcgggcag gttaccgaag gggcatttgg agcgtcaatc agccggagcg caggcgaatc
     4621 cccgtcgaat ggcccggtac ctgcgacgac gatggtagac tggcgccatg ttgaccggag
     4681 cgcacatgtc gatcgccggc ggcgtggata cttcggtggc gcgtggcatc gaagtgggct
     4741 gccgcacgat gcagatcttc accaagaacg ccaaccaatg gaccgccgcg cccatccacg
     4801 ccgacacggc caggagattc cgcgaggaat tggatcgctc ggggatcggg ccggtcgtct
     4861 cgcacgactc gtacctgatc aacctgggtt ctccggacct ggcgctgcgc gagaaatcga
     4921 tggccgcttt cgtggatgaa ctcgaccggg ccgagcaact cgggttggtg ggggtggtca
     4981 cgcatccggg cgcccacatg ggacagggtg aagaggcggg tctcgagcgt gtggccgcct
     5041 cccttcgcgc ggtcctcaag acgacccatc gtcacaaggt ggccatcctg atcgaaaata
     5101 ccgccggcca gggaacctcg ctcggctggg cggtgagtca tctggccaca cttctggccg
     5161 gcgtggaggg tcactcccgc atcggcttct gcctcgacac ctgccatctg cacgcggcgg
     5221 ggcacgacct ctcgaccgat gcctcctacg gggcgttcat cgaagcgttc gacgcggccg
     5281 tcggcctcga ccggcttcgc tgtttccaca tgaacgactc gctcaagccg ttcgggagtc
     5341 gcgtggatcg ccacacccac atcggtcagg gaacgatcgg cgaggaaccg ttccggcgca
     5401 tcatgtgcga tacccggctt gcccacgtgc ccaagatcct cgagacgccc aaggtcggct
     5461 cgggagtgac cgaagaccgg atgaacctgg ccaccctggc ccggctgggc accccaccgg
     5521 cgccggcgag gaagcgcaag gccggatgac gctggccgac ggcctgatcc tcttcctcct
     5581 cattgtttcg gccggcatgg gactggcgcg ggggttcgtc cgggagataa cgggcctggt
     5641 gggcctgctg gcggcgatcg ccttcggtgc gctcctgggg cccggcctgg cgggttcggt
     5701 gttcgggtgg atccccccac ccttcgccgg catcggggcc tttctcaccg tcttcctggc
     5761 ggtgctcttc atctcggcgc tcgtgggccg cctccttacg gccgccgcgc acgctgccag
     5821 cctctcgttt cccaaccggg tactcggagg cctcttcggc ctcgttcgcg ccgcctgctt
     5881 cctcctggcg ctcctgttcg cgctggacct ggtcggcgtc gatgcgggcc cctggctcaa
     5941 gggttcccgg ctcggcgaac cggcctggca ggcggtgcaa cgcctccgcg aggccacggg
     6001 cgcccacccg atggatgcgc cggtcaaacc gggcccgccc ggctccacca cgatctgacg
     6061 ctcctcggcg tccggggccg gcttgccgaa accaggcata aaaaaaggag agcggccgaa
     6121 gccgctctcc tcatttcctt tggggatccg attactcgga caggagcccc ttgatccggc
     6181 tccaggtgct cggcgccacc ggcaccgggc atccgaagaa gtccatcttg ccgtcggccg
     6241 aacggatgta gccggtcggg aagctgccgg gcgtccggtt ccacacgccg atcaggtaac
     6301 cgcagagggc gttgtcggtc tgcatgagac cgtacgccga gccgccggtg accgagatgt
     6361 cggcattgta gttgccgccg ttgttcagct cgttggacac cgtgtagaag ttgttgaacg
     6421 ttccggagag gaccaccgag ccgtcattga acgtggaggg cgaggtggca ttcggcgggt
     6481 tcgttccgaa cgcacggtcc ggcgagccgt ccgcgtagat ggtccaggcg ccaccggtgt
     6541 agtccgtccg gcggaccggg cccagatcaa cggtgccctg cgaggtcatc ccggtgaaca
     6601 cgaaggtgta ctcggtggcc ccgtcgaccg gattgagcga cgtgagaggc gagttgtacg
     6661 gaagcgtgat cttgccgacg atcgtcagga catttccggc caccgagttg tggggggcca
     6721 cgaaattggg ctcccacgcc gcggactccg gcccggtcca gtcgatcacc ggattggtgg
     6781 ctgccgacgc cgttccgctg agaacgacgg ccgcgaacag agcgatcagt gaagctgtac
     6841 ctttatggcg cattaccgcc ccctttcagg cctatgtcgg ggtcggtgcg gcgtctccca
     6901 ggtgggacgc ctggcaccgg cctgccgaag tctcagcgat ggatacggat gttcgagttc
     6961 gggatgtcga gcgacttgat ctggccccag gtcgacggcg tgacagccag cggatcctgg
     7021 agatagcact cgccgtcgat ctgccagtgg tagccgcagg gggtgttccc cgcgcggatg
     7081 ccgatggcgc cgaaggtcca gccggagcgc tgcccgaccg ggatgttgcc cagctggctg
     7141 ccaccggtcc agttggtgag accggccagg ttgccgtcac cggtggtggt gctgaacacg
     7201 acatcgaagc tcgcgaagtc gccatgaagg atgagggtgc cgtcgttgta cgtgaggcga
     7261 tcgaaaaacg gatcgcactc gccgaggagg ttgaacgtcc cgagggtgcc ggtggtgcgg
     7321 ctgtcctcgt agaagttgat ttcgccgttc acgtagctgg ccaccacgat cgtgccgaag
     7381 gtatcggcgc tggcgaagaa gagatcctcg ccgagcgtga acgtgtac
//
LOCUS       DASVOD010000555         3515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_922634, whole genome shotgun sequence.
ACCESSION   DASVOD010000555 DASVOD010000000
VERSION     DASVOD010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922634"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VF720_12280"
     CDS             <1..413
                     /locus_tag="VF720_12280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7880182.1"
                     /translation="PVPDAPIDSTPPPPPAIEPGAGEAAENEATTEASGGGGGEGLLL
                     PPVVLKAAWLEYPKEARKRKSEGDVELRILVNESGGVSAVEIEKGSSDPLLDQAAIDA
                     ARTMRFRAARQGTRPVAVWFNYQFSFRLPNSQRS"
     gene            496..966
                     /locus_tag="VF720_12285"
     CDS             496..966
                     /locus_tag="VF720_12285"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX7880183.1"
                     /translation="MNLTEVLDERAISLALDGTSMEEIVGGLVDLLAATDVIIDREAV
                     IRDILRREEVMSTGIGGGIAIPHAQSKGAARLAVAMGRSRREVDFNSLDGRPVQLFFL
                     VVGPEERGGYIRVLARISRLLYSGELQRRLAQARNSTDALMLIRAEEERVTHPA"
     gene            complement(989..2467)
                     /locus_tag="VF720_12290"
     CDS             complement(989..2467)
                     /locus_tag="VF720_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX7880184.1"
                     /translation="MMFNDDTPETQEQAAPAFDAEAITSPREVAAGEKVTGTVVKIGE
                     TTSFVDFGGRSEGSIETSEFKSEDGTLTVKEGDTIEATVLAVGDGITLTIGKAKGPSV
                     DLGFIDAASASGAPLEGTVKAVNKGGVVVDIGGVRAFCPVAQIDIAYVADPSAWVGRQ
                     LAFRVTQWDRDKKNLVVSRRALLQEERDRKGTETRGKLAVGQDLDGVVKRLAAFGAFI
                     DLGGVEGLAHVSELSRSRVANPSDVLKEGQSVRVKVIKIEDLSGAKERISLSLKALEA
                     DPWSSVAAQFQEGQVVEGNIVRLADFGAFVELAPGIDGLIHVSELGHGSVAHPRDAVA
                     TGDKVTARVQRIEPDRKRISLSLKALMPAPPPEPRTQVNIGTEVEGTVASVKNYGIFV
                     NLPSLGARVSGLVPRAETGESRGADLARRFPVGSPIKAEIIQVDEQGRIKLSMAAHAT
                     SSERRDLEEFKRSQPQRSEQPNMNPLAELLRPLKEKMESGQG"
     gene            complement(2771..>3515)
                     /locus_tag="VF720_12295"
     CDS             complement(2771..>3515)
                     /locus_tag="VF720_12295"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX7880185.1"
                     /translation="IAAAPRTLFRVADGTVEPNGVAALVLVTSGLFLLLGRWTGTVLG
                     GDLGTAVAADVRALPWRAIVAFLAIVPMVRGTLTALTADRTSRVARGLIEAQVGWFLA
                     GAASDTIRGASAAMIALITALPAFLAASRFPDRDDRAVTPPSHLALLATAVILFSLAG
                     LPGTAGFIGRWSLVEATRLGASWWLILPGVVLWGMNFVAIFRWIVTPPLRRDVTLTPS
                     VVILALVVLAGVLGGAALNDLLADVFPFY"
BASE COUNT          584 a         1155 c         1230 g          546 t
ORIGIN      
        1 cgccggttcc cgacgcgccg atcgattcca cgccgccgcc gccgccggca atcgaaccgg
       61 gcgccggcga agccgccgaa aacgaggcga cgaccgaggc ctctggtgga ggcggcgggg
      121 agggactgct cctcccgccg gtcgtcctga aggccgcctg gctcgagtac ccgaaggaag
      181 cacgaaagcg gaagagcgaa ggggatgtcg agctgcgcat cctggtcaac gagtcgggtg
      241 gcgtttcggc cgtggagatc gagaagggat cgagcgaccc gctgctcgac caggccgcga
      301 tcgatgcggc gcggacgatg cggttccgtg cggcgcgaca ggggactcga ccggtggccg
      361 tctggttcaa ctaccagttt tcatttcgat tgccgaacag ccagcgatct tgacatcgaa
      421 ttaatcggat cactaaagtg accctgcggg gccggcccgc ccggtatctt tcccctccta
      481 cccacatcga tgcccatgaa cctgaccgaa gttcttgacg agcgcgccat cagcctggct
      541 ctcgacggca cctccatgga ggagatcgtc ggggggctgg ttgacctcct ggcggcaact
      601 gatgtcatca tcgaccgcga ggcggtgatc cgcgacatcc tgcgccgcga agaagtgatg
      661 tcgaccggca tcggcggcgg catcgccatt ccgcacgcgc agagcaaagg agccgcacga
      721 ctggcggtgg ccatggggcg cagccggcgc gaggtggact tcaattcgct cgacgggcgc
      781 cccgtgcagc ttttcttcct ggtggtgggg cccgaggagc gcggggggta catccgcgtg
      841 ctcgcccgga tttcgcgcct cctgtacagc ggcgaactgc agcggcgcct tgcccaggcc
      901 cgcaactcga ccgacgcgct gatgctcatc cgcgcggaag aagaacgggt gacgcacccc
      961 gcctgagggc gcatcacccg atcctggatt acccctggcc gctctccatc ttctccttga
     1021 gggggcgcag cagttcggcc agcggattca tgttcggctg ttcggaacgc tggggctggc
     1081 tccgcttgaa ctcctcgagg tcgcgtcgct cggaactcgt ggcatgggcg gccatcgaca
     1141 gcttgatccg gccctgctcg tcgacctgga tgatctcggc cttgatgggg gatccgaccg
     1201 ggaaacgcct ggcgagatcc gccccgcgcg actcgccggt ttcggcccgc ggcacgagtc
     1261 cggaaacacg ggcgccgagc gacggaaggt tcacgaagat gccgtagttc ttcacgctgg
     1321 ccaccgtccc ctcgacttcc gttccgatgt tgacctgggt gcgcggttcc ggaggaggtg
     1381 ccggcatgag cgccttcagg gacaggctga tccgcttgcg gtccggttcg atgcgctgga
     1441 cgcgggccgt gaccttgtct ccggtggcga cggcgtcgcg cgggtgggcc accgagccat
     1501 gaccgagctc ggagacgtgg atcaggccgt cgatgccggg ggccagttcg acgaacgcgc
     1561 cgaagtcggc cagtcggacg atgtttcctt ccacaacctg cccttcctgg aactgcgccg
     1621 ccacgctcga ccagggatcg gcctcgagcg ccttgagtga caggctgatc cgctccttgg
     1681 cgccggagag atcttcgatc ttgatgacct tgacgcggac cgactgtcct tccttcagga
     1741 cgtcggacgg gttggccacg cgggagcggg agagctcgga aacgtgggcc agtccctcga
     1801 cgccccccag gtcgatgaag gcgccgaacg cggcgaggcg cttgaccacg ccgtccaggt
     1861 cctggccgac ggccagcttg ccgcgggttt cggtgccctt cctgtcgcgt tcttcctgca
     1921 ggagcgcacg gcgggagacg accagattct tcttgtcgcg atcccactgc gtgacgcgga
     1981 aggccagctg acgcccgacc caggccgacg gatcggccac gtaggcgatg tcgatctggg
     2041 ccaccgggca gaaggcgcgg acaccgccga tgtcgacgac gacgccaccc ttgttgacgg
     2101 ccttcacggt accctcgagc ggggcgccgg aggcggaggc cgcgtcgatg aagccgaggt
     2161 cgaccgacgg gcccttggcc ttgccgatcg tgagcgtgat gccgtcgccg acggcgagca
     2221 cggtggcctc gatcgtgtcc ccttccttca ccgtcagggt tccgtcctcg gacttgaact
     2281 cggacgtttc gatcgatccc tcgctgcggc cgccaaagtc gacgaagctc gtggtctcgc
     2341 cgatcttgac gacggttccc gtgaccttct cgcccgcggc cacttcgcgc ggggatgtga
     2401 tggcctcggc gtcgaaggcc ggggcggctt gttcctgggt ctcgggagtg tcgtcgttga
     2461 acatcatgtc gggatctccg ttaccaattc gtgacattcg cccagacgtt gacgaccacg
     2521 gggccgctgg gccggcgagc ctcgggttcg gctgaacgac cccggcatgg cggggcacgg
     2581 cgtggcggtg aacggaaccg gagatcactc gaaggcagaa ggtgccgagc gaaccggggg
     2641 tctgttacgg ggcctcgttc cgtggcaatc gtttccgggc ggaaacgacc gttatcgaca
     2701 gcccgcgaag gttagcagag gacccttcgc ggaaacaagc aacagggggc ggagaatcgg
     2761 ccccccgtcc tcagtaaaag ggaaagacgt cggccaggag gtcattcagc gcggctccgc
     2821 ccaggacgcc ggccagcacc acgagcgcga ggatgaccac cgaaggcgta agggtcacat
     2881 cccgccgcag gggcggggtg acgatccagc ggaagatcgc cacgaaattc atcccccaca
     2941 ggacgactcc gggaaggatc agccaccagg aggcgccgag gcgcgtggcc tcgacgaggg
     3001 accagcgtcc gatgaatccg gcggtgccgg ggaggccggc cagcgagaag aggatcaccg
     3061 ccgtcgcgag cagggcgagg tgggatggcg gcgtgacggc gcgatcgtcg cgatccggga
     3121 aacgcgatgc ggccaggaat gccgggaggg ccgtgatgag tgcgatcatc gcggcgctgg
     3181 cgccccggat cgtatcggag gccgccccgg ccaggaacca tccgacctgg gcttcgatca
     3241 gccccctggc cacgcgcgac gtgcgatccg cggtcagtgc ggtcaacgtt cctcgaacca
     3301 tcggcacgat ggccaggaag gccacgatgg cgcgccacgg cagcgcgcgg acatcggctg
     3361 ccaccgccgt gccgaggtcg ccgccgagga cggtgccagt ccagcgcccg aggaggagaa
     3421 agaggccgct cgtgaccagc acgagcgcgg ccacgccgtt cggctccacg gtgccgtcgg
     3481 ccacccggaa cagggtgcgc ggcgcggccg cgatc
//
LOCUS       DASVOD010000556         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_609347, whole genome shotgun sequence.
ACCESSION   DASVOD010000556 DASVOD010000000
VERSION     DASVOD010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609347"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="VF720_12300"
     CDS             complement(<1..963)
                     /locus_tag="VF720_12300"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEX7880186.1"
                     /translation="MPELSWASLRGRLGLEPREGTLFLLMGVLVATLLCAYTLAKVMR
                     DALFLDEFGAAALPYMYVGVALASAFFVWIEAFLSGRFSRGGATRATQWLAILLSVVA
                     ALSYPVAHRWTTAWFYLWTGSQAMMLLPHFWLLALDLWDSQRARHLFPVLSGFGLIGG
                     LLGGGVAAWLTPHIHRSGLLWTISALLVAAHGLTLLVQRYRERHEAPITRAPSGLTAR
                     DVIRRSTYIKVLIAALALSVIVSTMVDFQFKYFIKEIYQDPHDLTQFLGKFYIGLNVL
                     ALLFQFGVSGWLLQRLGLGASTGLQPATVIVFATMSALSGGFWAF"
     gene            1253..>1879
                     /locus_tag="VF720_12305"
     CDS             1253..>1879
                     /locus_tag="VF720_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880187.1"
                     /translation="MALFFLLLGAGVFEASAVRSQEPRFGDSTWVAPQADSIAGASGI
                     DTPGPRVAARDHERGWETALRAPFRLVFLPFRLLARGLEFGVSKFGGGFVEPKAPKPT
                     YGPRFGVRFEINDVATGLQDVGVGPAVAWAPQPDLKIRASAVASIRDRRHVLLRSVYR
                     DHQPTSLFLDGDYDFRPDRSFFGIGNDTEKEDRAFYRLEDTRLDGSIRI"
BASE COUNT          352 a          602 c          640 g          285 t
ORIGIN      
        1 gaaggcccag aagccgccgc tgagcgcgga catcgtggcg aacacgatga ccgtggccgg
       61 ttgcagcccg gtggaggcgc cgaggccgag gcgctgcagc agccagccgg acacgccgaa
      121 ctggaacagc agcgccagca cgttgaggcc gatgtagaac ttcccgagga actgggtcag
      181 gtcgtgcgga tcctggtaga tctccttgat gaagtacttg aactggaagt cgaccatcgt
      241 cgacacgatc accgagaggg ccagcgcggc gatcagcacc ttgatgtagg tcgagcggcg
      301 gatcacgtcg cgggcggtga gtcccgaggg cgcgcgggtg atcggggctt cgtgccgctc
      361 acggtagcgc tggacgagga gcgtgaggcc gtgcgcggcg acgagcagcg ccgaaattgt
      421 ccagaggagg ccggagcggt ggatatgggg cgtgagccac gcggccacgc caccgccgag
      481 caggccgccg atgaggccga agccgctcag caccggaaag aggtggcgcg cccgctggga
      541 atcccacagg tcgagcgcca ggagccagaa gtgcggcaac agcatcatgg cctggctgcc
      601 ggtccagaga tagaaccagg ccgtggtcca gcgatgcgcg acgggatagg acagcgccgc
      661 caccacgctc aggaggatgg ccagccactg cgtggcccgc gtggcgcccc cccggctgaa
      721 gcgtccggac aggaatgcct cgatccatac gaagaaggcc gatgccagcg ccacgcccac
      781 atacatgtat gggagagccg ccgcgccgaa ttcgtcgagg aagagggcgt cgcgcatcac
      841 cttggccagc gtgtacgcgc acaggagcgt ggcgaccagc acccccatca ggaggaaaag
      901 cgttccctcc cgtggctcga gaccgagccg gccgcgcagg cttgcccagc tcagttccgg
      961 catcccattc ctacctccgg tggggaaact cctgatgaca cgctcgtcgg ggccgcaaca
     1021 cagtcaccgt atgacgctgg tcgttggtcc aaggcttgcg tcacggtcga tcgactgtat
     1081 tgaagtggaa cagttcaccc ggcacatcga tgaatcctaa gggcctgacc gcaagatccg
     1141 ggccgtccga agggcgaacg tggccgcgca cgccctcgga aatcgatagt gaggcaacat
     1201 gatcgacgag agcggcacga cccgacgagt gtggtgcagg aatttttttc cggtggcact
     1261 ttttttcctg ctcctcggag ccggggtttt cgaagcgtcc gcggtccgtt cccaggagcc
     1321 ccggttcggc gattcgacgt gggtggcccc gcaggcggac tccattgccg gcgcatcggg
     1381 catcgacacg cccggccccc gcgtggccgc gcgcgatcac gagcgcggat gggagaccgc
     1441 tctgcgagcg ccattccgac tggtcttcct ccccttccgc ctgctggccc gcggcctcga
     1501 attcggcgta agcaagttcg gtggtggttt cgtggagccg aaggcgccaa agccgacgta
     1561 cgggcccaga ttcggcgtcc ggtttgaaat caacgacgtg gccaccggcc tccaggacgt
     1621 tggcgtcggg ccggccgtgg cgtgggctcc gcaacccgac ctcaagatac gcgcgagcgc
     1681 ggtggcgtcg atccgcgatc ggcgacatgt gttgcttcgc agcgtgtacc gcgaccatca
     1741 gccgacgagc ctcttcctcg acggcgatta tgacttccgc ccggaccgca gcttcttcgg
     1801 tatcggaaac gacaccgaaa aggaggatcg ggcgttctat cgcctcgaag acacgcgact
     1861 cgacggcagc atccgcatc
//
LOCUS       DASVOD010000557         3260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_89783, whole genome shotgun sequence.
ACCESSION   DASVOD010000557 DASVOD010000000
VERSION     DASVOD010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89783"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VF720_12310"
     CDS             <1..1296
                     /locus_tag="VF720_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880188.1"
                     /translation="IRITGRDCGTFGVLGLVVHAPPGSLLTTNLFATPSTPLTRDASV
                     SSRGATGAAVTDSLAATRGLLTKACSFLLASRPNEGCFSMAGSWYQSAYAIRTLLAGG
                     KVLGEPAFIDAASECLDRFVAEQRPDGGWSATYFGKPGCPRAAAEVSAAVSANLADGG
                     SAALALAVASAAVESPRREQYREALRRYADQVVLPNQLETGAFVNLRFMGEDHRHPYS
                     VATGIQAAHLSALYAVTGEARYLAAAERGAHFLLAGFQATGRYAFHRHDQPGAVNIQS
                     IRFGDLYYILEGLQWVHHYTGDVELSRAIEEAFDGYLWGQGGVSASRTNEVLWKANNE
                     WEASKQGALLGVLSDFHRMTPPAADEPAVQRRDRRRRDTGEWIRQFVEWLGRPENAEQ
                     LGFLVDVASPHGEYAFPATGFAGIGLASVIDPEALFPAR"
     gene            1314..3113
                     /locus_tag="VF720_12315"
     CDS             1314..3113
                     /locus_tag="VF720_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880189.1"
                     /translation="MTFLRFPAPAILTAAVWLLRFGSVPEARAADADTLLSLRPASGC
                     AVPELPETAYVSPPNRRRLLPIVFDNAGEGPLAPRSVRIGAQSRAPGYAWFDGVVRDR
                     LVSSETDPLFADLAVGHDATMVVAVDPPDSLYTVGVGLGDPLLPRGPVDVVANDSLVA
                     AGVSTTPGERRVLRFRVPARHGRIAVRFIARSCHGFGVSDLAIEGPSPSWLGQIFPPV
                     APVPDAVPPTTLLPAATPDTTRAILRRLGTFLVAHKPQGGGWSSWGLWYQTAFAVRGV
                     LSAGLVLDEPAWREAAFATIDRFVAEQSPAGTWSAGYVGADTCAGEAPPDTSSTNLAD
                     VGSMVLVLSLAAPDASPERREAWLAAARRYSDQVVLPEQLPDGAFPNRRWMGKDFLHP
                     YTVATATQCSQLMTLGAVTGEARYTAAAEKAAAWLTAGILPDGRVSLYPHDSANQKIL
                     ESIRFGDAFYLVEALVTVRDLTRDVKLKDACRKSVERWLTGPEGIKSKSLRGYWWAPM
                     DMWTSSKMAGVPWILARDPRRGGQPIIDNWLDRSIGWLADPMRAQLIGIGAHPASTRG
                     EYALTATGFAAMGVAAALDPAVLKPGAPTPVGR"
     gene            complement(3094..>3260)
                     /locus_tag="VF720_12320"
     CDS             complement(3094..>3260)
                     /locus_tag="VF720_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX7880190.1"
                     /translation="WMKGIVPDTDLGTPILGLDVWEHAYYLKYQNRRADYLAAWWNTV
                     DWSAVSARLA"
BASE COUNT          450 a         1184 c         1100 g          526 t
ORIGIN      
        1 atccgtatca cggggcgcga ctgcgggacg ttcggtgtcc ttggcctcgt cgtgcatgcg
       61 ccgccgggct cgctgctgac taccaacctg ttcgccacgc cgtccacccc cctcacgcgc
      121 gatgcatcgg tttcctcgcg cggcgcgacc ggcgcggccg taaccgattc cctggccgcc
      181 acgcgcgggt tgctcacaaa ggcctgctcg ttcctccttg cctcgcgccc caacgagggg
      241 tgcttttcaa tggcgggaag ctggtaccag tcggcctacg ccatccggac gctgctggcc
      301 ggcgggaagg tgctgggcga accggccttc atcgacgcgg ccagcgagtg cctcgaccgc
      361 ttcgtcgcgg agcagcggcc ggacggtggc tggagcgcca cctatttcgg aaagccaggc
      421 tgccctcggg cggcggccga agtgtcggct gcggtgagcg ccaacctggc ggacggcgga
      481 tcggccgcgc tggcccttgc cgtggcttct gccgccgtcg aatcgccgcg gcgcgagcag
      541 taccgcgagg ccctccgtcg ttacgcggac caggtggtgc ttcccaacca gctcgaaacc
      601 ggcgccttcg tgaacctccg cttcatgggc gaggatcatc gccatcccta cagcgtggcc
      661 accggcatcc aggccgccca cctgtcggcg ctctacgcgg tcacgggaga ggcgcgctac
      721 ctggccgcgg ccgagcgggg cgcccacttt ctcctggccg gcttccaggc gaccggccgc
      781 tatgccttcc accggcatga ccaacccggc gcggtcaaca tccagtcgat ccgcttcggc
      841 gacctctatt acatcctcga ggggctgcag tgggtccacc actacaccgg cgatgtggaa
      901 ctctcccgcg ccatcgagga agcgttcgac ggctacctgt ggggccaggg gggggtgagc
      961 gccagtcgca ccaacgaggt cctctggaag gcgaacaacg agtgggaagc gtcgaagcag
     1021 ggcgcccttc tcggggtgtt gagcgacttc caccgcatga ccccgccggc ggcggacgag
     1081 ccggctgtcc agcgtcgcga tcgacgccgt cgtgataccg gcgagtggat ccggcagttc
     1141 gtggagtggc tcggccggcc ggagaacgcc gaacagctcg gtttcctggt ggacgtggcc
     1201 tcgcctcacg gcgaatacgc ctttcccgcc accggctttg ccggcatcgg gctggcgtcg
     1261 gtgatcgatc cggaggcact cttcccggcg cgatgacgat cccccaccgc cgaatgacgt
     1321 tcctgcgatt cccggccccg gccatcctga cggccgccgt atggctgctg cggttcgggt
     1381 cggtcccgga agcccgggcg gccgacgccg acacgctcct ctcgctccgc cccgcctcgg
     1441 gctgcgccgt accggagctc ccggaaacgg cgtacgtgtc gccgcccaat cgccggcgcc
     1501 tccttccgat cgtgttcgac aacgccggtg aaggtccgct cgcgcccagg tcggtgcgca
     1561 tcggcgcgca gtcgcgcgcg cccggatatg cctggttcga cggcgtggtg cgcgaccgcc
     1621 tggtgtccag cgaaacggat cccctgttcg ccgacctggc cgtcgggcac gacgccacga
     1681 tggtcgtggc cgtcgatcca cccgacagcc tctacaccgt cggcgttggc ctcggcgacc
     1741 cgctccttcc ccgggggccg gtggacgtgg tggccaacga cagcctcgtg gcggcgggag
     1801 tcagcacgac cccgggcgaa cggcgcgtgc tccggttccg tgtgccggcc cggcatgggc
     1861 ggatcgcggt tcggttcatt gccaggtcgt gccacggatt cggcgtaagc gatcttgcca
     1921 tcgagggccc ttccccttcg tggctcggac agatttttcc ccccgttgcg ccggttccgg
     1981 acgccgtacc gcccaccacc ctccttcccg ccgccacgcc ggacaccacg cgcgccattc
     2041 tgcggcggtt gggcacgttc ctcgtggccc acaagccgca aggtggcggt tggtcgagct
     2101 gggggctctg gtaccagacg gccttcgcgg tgcggggcgt gttgtccgcc ggcctcgtgc
     2161 tggatgaacc ggcctggcgg gaagcggcct tcgccacgat cgaccgcttc gtggccgagc
     2221 agtctcccgc cggcacctgg tcggcgggtt acgtgggggc cgacacctgc gcgggagagg
     2281 cgccgcccga cacctcgagc accaacctgg ccgacgtggg atcgatggtc ctcgtccttt
     2341 cgctggccgc ccccgatgca tcgcccgaac gacgcgaggc gtggctggcc gccgcacgcc
     2401 gctactccga ccaggtggtg ctccccgagc agctcccgga cggcgctttc cccaaccggc
     2461 gctggatggg caaggacttt cttcacccct acacggtggc cacggccacg cagtgcagcc
     2521 agctcatgac cctgggcgcc gtgaccggcg aggcgcgcta caccgcggcg gccgaaaagg
     2581 cggcggcctg gctcacggcc ggcatcctcc ccgacggtcg cgtatcgctc tacccgcacg
     2641 actcggccaa tcagaagatt ctcgaatcga tccgcttcgg cgacgccttc tacctggtgg
     2701 aagcgctggt cacggtgcgg gacctgacgc gcgacgtgaa actgaaggat gcctgccgca
     2761 agtcggtgga gcgctggctc accggccccg aagggatcaa gagcaagtcc ctgcgcggct
     2821 actggtgggc gccgatggac atgtggacat cgtcgaagat ggccggcgtt ccctggatac
     2881 tggcccgcga tccgcgccgt ggcggccagc cgatcatcga caactggctc gaccggagca
     2941 tcggctggct ggccgacccg atgcgggccc agttgatcgg aatcggcgcg caccccgcct
     3001 cgactcgcgg cgagtacgcg ctgaccgcca cgggattcgc ggccatgggc gtggcggccg
     3061 cgctcgaccc cgcggtgctg aagcccggcg cgcctacgcc agtcgggcgc tgaccgccga
     3121 ccagtcgacc gtgttccacc aggccgccag gtaatcggcc cgccgattct ggtacttgag
     3181 gtagtaggcg tgctcccaca catccagccc gagaatcggt gtgccgaggt cggtgtcagg
     3241 cacgatgcct ttcatccagg
//
LOCUS       DASVOD010000558         5530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_713442, whole genome shotgun sequence.
ACCESSION   DASVOD010000558 DASVOD010000000
VERSION     DASVOD010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713442"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..838)
                     /locus_tag="VF720_12325"
     CDS             complement(131..838)
                     /locus_tag="VF720_12325"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX7880191.1"
                     /translation="MIVRIATFAGLRDDLGAPEVELTLPDGATAADLLAAVERRWPRL
                     TGRLGSVRVAADLEFLAASDPIPVDAELALIPPVSGGAPPDDEMIRKGSIVDPDVSVL
                     LEAAPIHLPALLHFVRTPSAGAVVTFTGTVREISRDRRVVELEYEAYHAMALEWLRRL
                     ASRSRGAFPVCRVAVAHRLGVVPVAEESVAIAVSSPHRAAAFDACRAVIEGLKADVPI
                     WKRERYHTGDEWVGWGS"
     gene            complement(835..1728)
                     /locus_tag="VF720_12330"
     CDS             complement(835..1728)
                     /locus_tag="VF720_12330"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX7880192.1"
                     /translation="MDADTLRCFLSVAELGSFTEAARRHFVTQPAVSLRLKSLEAEVG
                     TRLVERSGGRARLTPAGRLFEVRAAEALRQIDRGTAEVGDLLGLKRGRLAVGAIDAAG
                     IDLLPAPLKLFHERWPLVELVVRVEPSGPLVEALAAGTLDWAIVTLPLDRPGFVVETL
                     DEERLVLVSPPPGGTRSPGPAAVLERHPLIAYPRGSVTRGLIDTALEERGLEPRIAME
                     LSHPEAIRGLVEAGLGAAVLPERVVARGDKLVKSPAFTVRRRLGLVRRGTEEPSAAAR
                     AFRNVILAGRRRPRKDRILDA"
     gene            1862..3175
                     /locus_tag="VF720_12335"
     CDS             1862..3175
                     /locus_tag="VF720_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX7880193.1"
                     /translation="MSNTTVIRPATGKLGILLPGMGAVATTTIAGVEAIRQGLTPPIG
                     SMTQMGSIRLGKRTDNRNPRIKDFVPLAELSDVVFGGWDIFEDNAYEAAKKAGVLGYE
                     HLMQLKPFLEKVKPMSAVFDRHYVKKLDGPNVKTGANWYEKAQALMADIENFKKTTGC
                     DRLVAVWCGSTEIFLIEKPVHQSLKAFEEGMKKNDPDIAPSMVYAWACLMSGVPFANG
                     APNLTVDIPAIQELAREKHMPVAGKDFKTGQTLLKTVLAPMFKARYLGVSGWYSTNIL
                     GNRDGEVLDDPESFKTKEESKLGVLEHILQPQLYPQLYGNMYHKVRINYYPPRGDNKE
                     GWDNIDIFGWLGYPMQIKVDFLCRDSILAAPIVLDLALFMDLAQRSGMRGTQEWLSFY
                     FKSPMTAPSLYPEHDLFIQMMKLKNTLRNLKGEELITHLGTEYYD"
     gene            3376..3942
                     /gene="efp"
                     /locus_tag="VF720_12340"
     CDS             3376..3942
                     /gene="efp"
                     /locus_tag="VF720_12340"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX7880194.1"
                     /translation="MPIPATQLKVGMHIVHNGELHRVTSVMHVTPGNWRGMVQTKLMR
                     VKTGSQVEHRFRSVDTVDRAVLEQHELQYMYRSGDEFTFMNTESYEMHNLDTETLGDA
                     AKYLQENMMIQANYHEGKVIGIDVPMTIVVKIVECDPPMKGATASGGPKPAILEGGIT
                     VKVPQHLSVGDTVKIDTRDDSFIERVSN"
     gene            4063..4518
                     /locus_tag="VF720_12345"
     CDS             4063..4518
                     /locus_tag="VF720_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880195.1"
                     /translation="MGDHNKVVVRYRNGEVLKGTTQDFFPNRPVFHLLPLAGGQALEV
                     RTSELKAVFFVKDFNGHPERQDVPGFLAAPGTMNQGRKIAIRFQDAEVICGYSVTFTP
                     SRDGFFVFPADAEGNNLRIYVLTAATSDIKVGPAAESLVRTLLKGPYAA"
     gene            4618..>5530
                     /locus_tag="VF720_12350"
     CDS             4618..>5530
                     /locus_tag="VF720_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HEX7880196.1"
                     /translation="MAPAADAAAPARYLRWDGPISPAAADYLDREIQAAERDGAPLVI
                     IGIDTPGGLDTSMRRIVRAIAASPVPVAAWVGPSGSRAASAGCVIGLAAHVLAMAPGT
                     NIGAAHPVAVGGGAVDSTMAGKVANDAEAYVAALARQRNRNESWAREAVTKSLSASAE
                     EAVRLGVADLVAVDPADLLHKLDGRSLATPGGTRTLSTLGLGIEERHRDWRTDLLALL
                     TDPNVAYILLLLGMYGLFFELANPGALFPGVFGIVSLILGLYGLSNLPVSTAGLLLIL
                     AGLSMLLLEIKIASHGLLAIGGVVALAS"
BASE COUNT          980 a         1915 c         1779 g          856 t
ORIGIN      
        1 gttcggcgtc ggcattgaat ggaagcggct gaatttcgac tacggtgtga cggtcgacga
       61 gaccgacggg cccgggacta cccaccggtt cggcctcaac gtcctcctgg gacgtccccg
      121 ctaggcgggg tcacgacccc cagcccaccc actcgtcccc ggtatggtag cgctcccgct
      181 tccagatcgg cacgtcggcc ttcaatccct cgataaccgc ccggcaggca tcgaaggccg
      241 ccgcgcggtg cggcgatgat accgcgatgg ccaccgattc ttcggcgacg ggcaccacgc
      301 ccagccgatg cgccaccgcg acgcggcaga ccggaaacgc gccgcgcgat cgcgaggcga
      361 ggcggcgcag ccactcgagg gccatcgcat ggtaagcctc gtactccagt tcaacgacgc
      421 ggcggtcacg cgaaatctcg cgcacggtac cagtgaacgt gacgacggcg ccggcggagg
      481 gggtgcgcac gaagtggagc agcgcgggca ggtgtatcgg ggccgcttcg agcaacaccg
      541 acacatcggg atccacgatg gaccccttcc gaatcatttc gtcatccggg ggagctcccc
      601 cgctcaccgg cgggatgaga gcgagctccg catcgacggg gatcggatcg gatgccgcga
      661 gaaattcgag atcggccgcc acgcgcacgg agcccaggcg cccggtcaac ctgggccagc
      721 gacgctcgac ggcggcgagg agatcggccg cagtggctcc atcgggaagc gtaagctcga
      781 cttccggcgc tcccaggtcg tcacggagcc cggcgaaggt ggcgatgcga acgatcatgc
      841 gtcgagtata cggtccttcc gcgggcgccg gcgtcccgcc aggatgacgt tccggaaggc
      901 ccgggcggcg gccgacggtt cctccgtccc ccggcgcacc aggccgagcc ggcgtcgcac
      961 cgtgaaggcc ggcgacttga ccagcttgtc accccgggcc acgacgcgtt cgggcaacac
     1021 cgccgcgccg agaccggcct ccaccagtcc acggatggcc tccgggtgcg agagctccat
     1081 ggcgatgcgg ggctccagcc cccgttcctc cagtgcggtg tcgatgagcc cgcgggtgac
     1141 cgagcctctc gggtaggcga tcaacggatg ccgctccagc acggcggccg ggccgggtga
     1201 gcgagtccct cccggagggg gcgacacgag gacgagccgc tcttcgtcga gggtttcgac
     1261 cacgaatccg ggccggtcga gcggcagcgt gacaatggcc cagtcgaggg ttccggcggc
     1321 aagcgcctca accagcggcc ccgacggctc gacacggacc accagctcga ccaggggcca
     1381 gcgttcgtga aagagcttga ggggagcggg caagaggtcg attccggccg catcgatggc
     1441 cccgaccgcc aggcggccac gcttcaatcc gaggaggtcg cccacctccg cggtcccccg
     1501 atcgatctgg cggagtgcct cggcggcccg cacctcgaag agtcggccgg cgggtgtcag
     1561 gcgggcccgc ccccccgacc tctcgacgag tcgcgttccg acctcggcct cgagcgactt
     1621 gaggcggagg ctgaccgccg gctgggtgac gaagtggcgc cgggccgcct cggtgaagct
     1681 ccccagctcg gccaccgaca ggaagcagcg gagagtatcg gcatccatgc ggtaattata
     1741 agcgatactt atacaccgga tcaaatagat aaatttgact gacggttccg ggcggggcat
     1801 ccttgccgcc ggctcgattc ggacgcatgg cgcgttcctg ggccgggagg ttttccccga
     1861 tatgtcgaac acgaccgtca tccgccccgc caccggcaag ctcggcatcc tgctgccggg
     1921 tatgggcgcc gtcgccacca ccaccatcgc cggtgtggaa gccatccgcc agggactcac
     1981 cccgccgatc ggcagcatga cccagatggg ttcgatccgc ctcggcaagc gtaccgacaa
     2041 ccgcaatccc cgcatcaagg acttcgtgcc gttggctgag ctctccgacg tggtgttcgg
     2101 cggctgggac atcttcgagg acaacgccta cgaggcggcg aagaaggccg gcgtgctcgg
     2161 ctacgagcac ctgatgcagc tgaagccgtt cctcgagaag gtcaagccga tgtcggccgt
     2221 cttcgaccgg cactacgtga agaagctcga cgggccgaac gtgaagaccg gcgccaactg
     2281 gtacgagaag gcccaggcgc tcatggccga catcgagaac ttcaagaaga ccaccggctg
     2341 cgaccgcctc gtggcggtct ggtgcggctc gaccgagatc ttcctcatcg agaagccggt
     2401 gcaccagtcg ctcaaggcgt tcgaggaggg aatgaagaag aacgatcccg acatcgcccc
     2461 gagcatggtg tacgcctggg cctgcctgat gagcggcgtg ccgttcgcca acggcgcccc
     2521 gaacctgacg gtggacatcc cggccatcca ggaactggcc cgcgagaagc acatgccggt
     2581 ggccggcaag gacttcaaga ccggccagac gctgctcaag acggtgctgg cgccgatgtt
     2641 caaggcgcgc tacctcggcg tctccggctg gtactcgacc aacatcctcg gcaaccgcga
     2701 cggcgaggtg ctcgacgacc cggagtcgtt caagacgaag gaggagtcga agctcggcgt
     2761 gctcgagcac atcctccagc cgcagctcta tccgcagctg tacggcaaca tgtaccacaa
     2821 ggtccggatc aactattacc cgccgcgcgg tgacaacaag gaaggctggg acaacatcga
     2881 catcttcggg tggctcggct acccgatgca gatcaaggtc gacttcctct gccgcgactc
     2941 catcctggcc gccccgatcg tgctcgacct cgccctcttc atggacctgg cccagcgctc
     3001 cggcatgcgc ggcacgcagg agtggctgtc gttctacttc aagagcccga tgacagcccc
     3061 gagcctctat cccgagcacg acctcttcat ccagatgatg aagctcaaga acacgctccg
     3121 gaacctgaag ggcgaggagc tcatcaccca cctcgggacg gagtattacg actgaggcag
     3181 tcagacgaac gaagttcgtc tgaccgtccg ttgcccttgg acatcgtcag ggcgggagtt
     3241 ccgcaaggca ctcccgccca ttttttgacc tctgcgtcgc aatcctgcta aactctcccg
     3301 tcctttttgg agtgatggct tcgaccgagt cgtaccgccc tccgttcgcc tgatcctcaa
     3361 ccgaggtttt gtcacatgcc gattcctgcc acccagctca aggtcgggat gcacatcgtc
     3421 cacaacggcg agctgcaccg cgtcacgagc gtcatgcacg tcacccccgg caactggcgg
     3481 ggaatggtcc agaccaagct catgcgggtg aagaccggca gccaggtcga gcaccgcttc
     3541 cggtcggtcg acacggtgga ccgcgccgtc ctcgagcagc acgagctgca gtacatgtac
     3601 cgctcgggcg acgagttcac gttcatgaac accgagtcgt acgagatgca caatctcgac
     3661 acggagaccc tgggcgacgc ggcgaagtat ctccaggaaa acatgatgat ccaggccaac
     3721 taccatgagg gtaaggtcat cggcatcgac gtcccgatga cgatcgtcgt gaagatcgtg
     3781 gagtgcgacc ctcccatgaa gggcgccacc gcctcgggtg gtcccaagcc ggccatcctg
     3841 gaggggggaa tcaccgtgaa ggtcccgcag catctctcgg tcggcgacac ggtcaagatc
     3901 gatacgcggg acgacagctt catcgagcgg gtcagcaact gaggcccctg cccgggttat
     3961 ccccggggtc ggcacacccc actccgggct aaaccaccgg aggagtccgg ccgataaccg
     4021 gtccgagccg tcgcacggat acgggattcg gagccatcga ccatgggcga tcacaacaag
     4081 gtcgtcgttc gctatcggaa cggcgaagtc ctcaagggaa ccacccagga tttcttcccc
     4141 aaccgtcccg tattccacct cctgccgctg gccggcgggc aggccttgga agtgcggacg
     4201 agcgagctca aggccgtctt cttcgtgaag gacttcaacg gccacccgga gcgccaggat
     4261 gtgccgggtt tcctcgccgc ccccggcacc atgaaccagg gacgcaagat cgccatccgc
     4321 ttccaggacg ccgaagtcat ctgcggctac tcggtcactt tcacgcccag ccgagacggt
     4381 tttttcgttt tcccggcgga cgcggagggg aacaacctcc ggatctacgt gctgaccgcc
     4441 gcgaccagcg acatcaaggt cggacccgcc gccgagtcgc tcgtccgcac cctcctcaag
     4501 gggccttacg ccgcctgata ggcgcgccct tcccctgcta gactgcggtg tatgccgcac
     4561 ttcggctgga ccctccgcgc tgcactcatg gcggctctgc ttgccccacc gcggctcgtg
     4621 gcacccgcag cggacgcggc cgccccggca cgttatcttc ggtgggacgg ccccatcagc
     4681 cccgcggcgg ccgactatct cgaccgggaa atccaggccg ctgaacggga cggcgccccg
     4741 ctggtgatca tcgggatcga cacaccgggc gggctcgata cctcgatgcg gcggatcgtg
     4801 cgggcaatcg ccgcctcgcc cgttccggtg gcggcctggg tggggccatc gggatcacgc
     4861 gccgcctcgg ccggctgcgt catcggcctg gcggcccatg tgctggccat ggcacccggg
     4921 accaacatcg gcgccgcaca cccggtggcg gtgggcggag gcgccgtcga ctccacgatg
     4981 gccggcaagg tggccaacga cgcggaggcc tacgtcgcgg cgctggcgcg ccagcgaaac
     5041 cggaacgaga gctgggcccg cgaagcggtg acgaagagcc tctccgcgtc ggcggaggaa
     5101 gcggtgcgcc tcggcgtggc ggacctggtg gcagtcgacc ccgccgatct cctccacaag
     5161 ctcgatggcc ggagcctcgc aactcccggg gggacccgga ccctgtccac cctgggcctc
     5221 gggatcgaag agcggcaccg ggactggcgg accgaccttc tcgccctcct cactgacccg
     5281 aacgtggcgt acattctcct cctcctgggg atgtacggcc tcttcttcga gctggccaat
     5341 cccggggcac tcttcccggg tgtcttcggc atcgtctcgt tgatcctcgg actctacggc
     5401 ctgtcgaacc tgccggtatc gacggccggg ctgctcctca tcctggcagg gctctcgatg
     5461 ctccttctcg agatcaagat cgccagtcac ggcctcctgg ccatcggggg cgtggtggcg
     5521 ctggcgtccg
//
LOCUS       DASVOD010000559         4814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_871191, whole genome shotgun sequence.
ACCESSION   DASVOD010000559 DASVOD010000000
VERSION     DASVOD010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871191"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /gene="hydA"
                     /locus_tag="VF720_12355"
     CDS             complement(<1..1019)
                     /gene="hydA"
                     /locus_tag="VF720_12355"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX7880197.1"
                     /translation="MATVLIKNARIINAADRIEADIFIDGGTIKRIEKGIDLPADRVL
                     DATGKLALPGGIDVHTHLDMPFGGTFSSDDFHTGHVAAAFGGTTTHLDFSIQGQGEGL
                     YPALERWHAKAEGKAVIDYGFHMIVREMNDTVLADMGSLATKEGVNTFKFFMAYPGVF
                     MVDDALIFKAMLKARDVGGLIMMHAENGGSIDTLVKQALANGQTEPKYHALTRPPLCE
                     GEATGRAIDLAHLAGAPIYIVHVTCFHALERIRRARDAGWRVYGETCPQYLVCSYEDY
                     ERPNFEGAKFVMSPPLRDKWHAIKLWQGLERNGLQVVSTDHCPFFFDDPQGKQLGRND
                     FSKIP"
     gene            complement(1024..2160)
                     /locus_tag="VF720_12360"
     CDS             complement(1024..2160)
                     /locus_tag="VF720_12360"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="NADH-dependent; catalyzes the conversion of
                     pyrimidines to 5,6-dihydro compounds in pyrimidine
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HEX7880198.1"
                     /translation="MSVDLSTTFTGIRFPNPFLLASAPPTESESNILRAFDSGWGGVV
                     VKTIGMHPVINVAGPKTKFNRYDPTTGNFSMKTKSHIVHSSWNWELISDKPLDEWLPR
                     IRRIKAAHPDKVLIASIMAGSGSEKELDHWRTLATGCQEAGADGLELNLSCPHMDRPD
                     MGSNIGKDQALCSFVTQTVKEAARIPVWAKLTPATADIVVEAGAVFRGGGDAVVSSNT
                     FPSLPIVDPNTLEFEINVDGFVSSGGLGGPAIMPMSMANMARMTKAYPDKSFSAIGGV
                     SEFAHALNYMLLGAGTVQVCTAAMLDKAVGPGVIKRLNEGYAEFLEKNAHRGWKTVDD
                     FRGLLRDKVVKQSEIRRPDESGYQGGYDAEGYSGAEEVPATTKG"
     gene            complement(2312..3181)
                     /locus_tag="VF720_12365"
     CDS             complement(2312..3181)
                     /locus_tag="VF720_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7880199.1"
                     /translation="MPRVVRCGLIQAKNVLGPKSGLPAIKKAMVDKHEKLIVEAAKKK
                     VKILCLQELFYGPYFCAEQETHWYQMTEKVPDGPTTKLIQKLAKKHSMVIVAPVYEED
                     QPGVYYNTAAVIDADGKYLGKYRKTHIPHCQPGFWEKFYFRPGNTGYPVFETAVGKVG
                     VYICYDRHFPEGARALGLNGAEIVFNPSATVAGLSEYLWKLEQPAHAVANGYFIGAIN
                     RVGVEAPWKIGEFYGQSYFCDPRGQFLATGPRDKDAVVTADLNLDQIEEVRSVWQFYR
                     DRRPDAYGPLTQP"
     gene            3302..4618
                     /locus_tag="VF720_12370"
     CDS             3302..4618
                     /locus_tag="VF720_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7880200.1"
                     /translation="MFTNLTRGRTRLLPAILLSLVVVTPSSARNVRLAPTGKPVDYER
                     VRKALGSLAPGDTLTLMRGTFDWSANLSDSSLVAKQPGGMPIPVPRVVVRGEPAQPGR
                     AGVAGTLLKGAVDDDGRPARPKRGTNAAFRNAPNADGVTIEDLTFDSFENAIVLIQAD
                     TLVSATPVDALRNGTRDWTLQRLVVKGGPFGIMANGRHEGLTIRDCEFTMALPPHETK
                     PGPAPREGSFAVAVRPYPPAYPGLPTGVVIENNRTHGPVRERESEIFGGLILTSTNGR
                     LVGNEVTAWGIGMVVEGDSLDVSNNRINDCRIGIVAWSTDRLGTGTSHARIAGNEIRD
                     TRRQATGFLSDFTGTGLFLAGLRDSRIEDNRFKGNAGPDIVFGALPATKSSSGNVLAG
                     NGGTIVLTDASRQSNTISGSQLRVLGNRPNPPIVPDSSYVTTPKKP"
BASE COUNT          784 a         1558 c         1623 g          849 t
ORIGIN      
        1 ttcgggatct tcgagaagtc gttccggccg agctgcttcc cctgcgggtc gtcgaagaag
       61 aaggggcagt ggtcagtgct gaccacctgc aggccgttcc gctcgagccc ctgccagagc
      121 ttgatggcat gccacttgtc gcgcagcggc ggcgacatga cgaacttggc tccctcgaag
      181 ttcgggcgct cgtaatcttc gtaggagcag acgaggtact gcgggcacgt ctcgccatac
      241 acgcgccagc cggcatcgcg cgcgcgccgg atgcgctcga gcgcatggaa gcaggtgacg
      301 tgcacgatgt agatcggcgc gccggccagg tgcgccaggt cgatggcccg gccggtggcc
      361 tccccttcgc agagcggcgg ccgcgtgagc gcgtggtatt tgggctcggt ctggccgttg
      421 gccagcgcct gcttcaccag cgtgtcgatg gagccgccat tctcggcgtg catcatgatg
      481 aggccgccga catcgcgcgc cttgagcatg gccttgaaga tcagcgcgtc atccaccatg
      541 aagacgcccg ggtaggccat gaagaacttg aacgtgttca ccccttcctt cgtggcgagc
      601 gagcccatgt cggcgaggac ggtgtcgttc atctcccgca cgatcatgtg gaagccgtag
      661 tcgatgacgg ccttcccctc ggccttggcg tgccagcgct cgagcgccgg gtagagccct
      721 tcgccctggc cctggatgga aaagtcgagg tgcgtggtgg tgccgccgaa ggccgccgcc
      781 acgtggccgg tgtggaagtc gtcggaggag aaggtgccgc cgaagggcat gtcgaggtgg
      841 gtgtggacat caatcccgcc gggaagcgcc agcttcccgg tggcatcgag gacgcggtcg
      901 gccggcaggt cgatcccctt ctcgatccgt ttgatggtgc cgccgtcgat gaagatgtcg
      961 gcctcgatcc ggtccgccgc gttgatgatc cgcgcgttct tgatcagtac ggtggccatg
     1021 ccatcaacct ttcgtcgtcg ccggtacttc ctcggccccc gaataccctt ccgcgtcata
     1081 cccaccctgg tagcccgact catcgggccg gcggatttcg ctctgcttga ccaccttgtc
     1141 gcgcagcagg ccgcggaaat cgtccacggt cttccagccg cggtgggcgt tcttctcgag
     1201 gaactcggca tacccttcgt tgagccgctt gatcaccccc ggccccaccg ccttgtcgag
     1261 catggcggcc gtgcacacct gcacggtgcc ggcgccgagg agcatgtaat tgagcgcgtg
     1321 cgcgaactcc gacacgccgc cgatggccga gaacgacttg tcgggatagg ccttcgtcat
     1381 gcgcgccatg ttagccatcg acatgggcat gatggccggt ccgccgaggc cgccgctcga
     1441 cacgaaaccg tccacgttga tctcgaattc gagcgtgttc gggtccacga tcgggagcga
     1501 cgggaacgtg ttggagctga ccacggcgtc gccgccaccg cggaagacgg cgccggcctc
     1561 caccacgatg tcggcggtgg ccggcgtgag cttggcccac accgggatcc tggcggcttc
     1621 cttgaccgtc tgcgtgacga acgagcagag cgcctggtcc ttgccgatgt tcgagcccat
     1681 gtcaggccgg tccatgtgcg ggcacgacag gttgagctcg aggccgtccg ccccggcttc
     1741 ctggcagccg gtggccagcg tgcgccagtg atccagttcc ttctcgcttc ccgagccggc
     1801 catgatggag gcgatgagca ccttgtcggg atgggcggcc ttgatgcggc ggatgcgcgg
     1861 cagccattcg tccagcggct tgtcgctgat gagctcccag ttccacgacg agtggacgat
     1921 gtggctcttc gtcttcatcg agaagttgcc ggtggtcgga tcgtagcggt tgaacttggt
     1981 cttgggcccg gccacgttga tgacgggatg catgccgatc gtcttgacca ccaccccacc
     2041 ccacccggaa tcgaaggcgc gcaggatgtt gctttccgat tcggtcggcg gtgcggaggc
     2101 caggaggaag gggttgggaa aacggatgcc ggtaaaggtg gtcgacaggt cgacgctcat
     2161 ggattctccg atcgggcggt cgtggccgcc cgtggccggc tcgcatccgt cgggcggtcc
     2221 gggcgtggtg gggtcgccgg gacgatggcc gatccggaag tcatgccaag ccaatgagaa
     2281 aggttaacac agggctccgg ggttgccccg ctcacggctg ggtgagcggg ccgtacgcgt
     2341 ccgggcgacg atcgcggtag aactgccaga ccgagcgcac ttcctcgatc tggtcgagat
     2401 tgaggtcggc cgtgactacg gcgtccttgt cccggggacc ggtggccagg aactggccgc
     2461 gcggatcgca gaaatagctc tggccgtaga actcgccgat tttccagggg gcctcgacgc
     2521 cgacgcggtt gatggcgccg atgaagtagc cattggccac cgcgtgggcc ggctgctcga
     2581 gcttccagag gtattcggag agtccggcca cggtggccga gggattgaag acgatctcgg
     2641 cgccgttcag gccgagcgcg cgggcgccct ccgggaagtg gcggtcgtag cagatgtaca
     2701 cgccgacctt gccgaccgcg gtctcgaaga ccgggtagcc cgtgttcccg ggtcggaagt
     2761 agaacttctc ccagaagccg ggctggcagt gcgggatgtg ggtcttccgg tacttgccga
     2821 ggtacttgcc gtcggcgtcg atcacggcgg ccgtgttgta gtagacgccg ggctggtcct
     2881 cttcatagac gggggccacg atcaccatgc tgtgcttctt cgccagcttc tggatgagtt
     2941 tcgtggtggg gccgtccggc accttctcgg tcatctggta ccagtgggtt tcctgctcgg
     3001 cgcagaagta ggggccgtag aacaactcct ggaggcagag gatcttgacc ttcttctttg
     3061 cggcttcgac gatcagcttc tcgtgcttgt cgaccatggc cttcttgatg gcgggaagcc
     3121 ccgacttggg tcccaggacg ttcttggcct ggatgaggcc gcaacggacg acgcgaggca
     3181 tgacgggatc tcctctggag gtggccgacg gttgaaacgg tgaaagctag caggcatcgc
     3241 cccgatgtga aagcggaagg cgattgaccc ggggaacagc cccgggggat agtttccggc
     3301 catgttcacc aacctgaccc gcgggcgaac ccgcctgctt cccgcgatcc tcctgtcact
     3361 cgtcgtcgtt acaccgtctt cggcacgcaa cgtccgtctt gccccgaccg gaaagccggt
     3421 cgactacgag cgtgtccgga aggcgctcgg ttcgcttgcc cccggcgaca ccctcaccct
     3481 gatgcggggg acattcgact ggtcggccaa cctctccgac tcgtccctgg tggcgaagca
     3541 gccggggggg atgccgatcc cggtgccgag ggtcgtcgtc cgcggcgagc ccgcccagcc
     3601 gggacgcgcc ggtgtagccg gcaccctcct gaagggggcc gtcgacgacg acgggcgccc
     3661 ggcccggccg aagcgcggca ccaacgccgc cttccggaac gcgccgaatg ccgacggcgt
     3721 gacgatcgag gacctgacgt tcgattcgtt cgagaacgcc atcgtcctca tccaggccga
     3781 cacgctcgtc tccgccacgc cggtggacgc gctccggaac ggcacgcggg attggacgct
     3841 ccagcggctg gtcgtgaagg ggggcccttt cggcatcatg gccaacggcc ggcacgaagg
     3901 cctgaccatc cgcgactgcg agttcaccat ggctctcccg ccgcacgaga cgaagcccgg
     3961 tcccgcgccg cgcgaagggt cgttcgcggt ggcggtgcgc ccctaccccc ccgcctatcc
     4021 ggggctcccc acgggcgtgg tcatcgagaa caacaggacc cacggccccg tgcgggagcg
     4081 cgaatccgaa atcttcggcg gcctcatcct cacctcaacg aacgggcgtc tcgtcggcaa
     4141 cgaggtgacg gcctggggga tcggcatggt ggtggaggga gactcgctcg atgtctcgaa
     4201 caacaggatc aacgattgcc ggatcggcat cgtggcctgg tcgaccgacc gcctgggcac
     4261 cggcaccagc cacgcccgca tcgccggcaa cgaaattcgc gacaccaggc gccaggccac
     4321 cggcttcctc tccgacttca ccgggacggg tcttttcctg gccggcctca gggactcgcg
     4381 catcgaggac aaccgcttca agggaaacgc gggccccgac atcgtcttcg gcgccctccc
     4441 cgcgacgaag agctcgagcg gcaacgtgct ggccggcaac ggcggcacga tcgtgctcac
     4501 cgacgccagc cggcagagca acacgatctc ggggtcgcag ctgcgggtgt tggggaatcg
     4561 acccaatccg ccgatcgtgc cggattcgag ctacgtgacg acgccgaaga agccctagct
     4621 agtcggcgct gcagagcagc ggcgaccgtt ttttgggaag tgaactccgg cgccaacaat
     4681 gtcgaaagac aattgatagt gaagaaaagg ccccccgccg aaggcggagg gccttttcac
     4741 gattaccctg tcattttgac atcgtgggcg ccgaactcac tacgacaaca gcgacgctgt
     4801 cagctcggac gccc
//
LOCUS       DASVOD010000560         7511 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_245844, whole genome shotgun sequence.
ACCESSION   DASVOD010000560 DASVOD010000000
VERSION     DASVOD010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245844"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            394..1599
                     /locus_tag="VF720_12375"
     CDS             394..1599
                     /locus_tag="VF720_12375"
                     /inference="COORDINATES: protein motif:HMM:NF018439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HEX7880201.1"
                     /translation="MRRRHVICLLSLLVPTAAPAASPGVGSIQLFRPLTADPRENQFR
                     MAFSDYTENWRYGTDITDSTSQGGFEERSGTRWDVGIGATLRLDPMRRIFGWKGPWHR
                     YQLGLPAGVFARFDNGTKELIDTDFQFGGSLDMLWAGEWADSTGVTTFDGPVVTSRIT
                     LFHRSTHLGDEYLASGDFGDNQEGHPDEGSLFHHPPVKRVDLTYEAVRAIVSTEWAPG
                     FINGGRSTIRLYGGGEFKVALRPRTPADFSSPIAQAGVELRSAGNQDDPSDGWMTRLL
                     NAPFRRPFFETEWFLAFDWKLAKPYDFASCDNPDGSGEVWTPHLWTDCRHGHEFEGYA
                     GSWHGMVGLSLYPGTGRRVEEGGRRQSPEIMLALEWYRGYAPDGQFLDQRLEAHPLAY
                     VLPGITIHF"
     gene            1651..1779
                     /locus_tag="VF720_12380"
     CDS             1651..1779
                     /locus_tag="VF720_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880202.1"
                     /translation="MSKLLRGLVAGYGAKKLGGGCFSTIIVFLLLWWLLGHFNVFR"
     gene            complement(1763..2389)
                     /locus_tag="VF720_12385"
     CDS             complement(1763..2389)
                     /locus_tag="VF720_12385"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEX7880203.1"
                     /translation="MHRRHALVASLAALILSSAILSTGCSRNDAGSDVRSGTSSAEVP
                     KPPPVEKPAPEKPAEPAADYDLRFIDTMSRHHKDAVAMARLAASQASRAEIQEFAAKV
                     ITDQERQIELMKGWRDQWYSGHSNAGGDMPGMERMGTGGLEGLSGTDFDAKFIEMMTS
                     HHQGGVKMATSGLEKLQHAELKALAQQIADVQTQEIAKMAQWASTGTR"
     gene            complement(2448..3812)
                     /locus_tag="VF720_12390"
     CDS             complement(2448..3812)
                     /locus_tag="VF720_12390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEX7880204.1"
                     /translation="MNRRNDDTAPPARTDAGHKGPLDRFLGIFADVKGGEGFTALLLM
                     FNIFLLLASYYLLKTIREPLILAGNGGAEIKSYAAAAIAGLLVILVPAYSALASRVSR
                     VKLINGVTLIFIACLVAFFVLNRAGVAVGVPFFIWVGIFNVMMIAQLWAFANDVYTVE
                     QGKRLFAIVGVGASLGAIAGSFATGQLVKQYGPYPFMLAAAVLLGVCMFLTNLIHMRE
                     KAHAAGKSPGDPAKKEDVGETIGGKSGFALVLSDRYLLMIGLLMLVVNLVNTTGEYIL
                     GKTVVDLLSHGQGGGADEKKLIGEFYGNYFTIVNIISALLQAFVVSRVIKYFGVRTAL
                     MVMPVVAFAGYAAMAFIPVLSFIRGVKMAENSLDYSLQNTTRNALYLPTSREAKYKAK
                     QANDTFFVRAGDVLSAGLVYAGTTWLNFASKQFALVNAALVLVWLGLAFALGRSFKRL
                     AKSD"
     gene            complement(3853..5292)
                     /locus_tag="VF720_12395"
     CDS             complement(3853..5292)
                     /locus_tag="VF720_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX7880205.1"
                     /translation="MSPVVTDSAGATETKAFVGPVAPWTPLFSDTLIGLVPGTEDTSA
                     RFLGRLRTGYTADTLNIILFGDNRPSWRTARLKPETTRLKAMWSLNPVKWVKGLITLP
                     VFLFKGLYPDLALIRDIPNLITNKPSYGREEQVLKAIGVLVDSLEARKKTVAAIINTG
                     DLVKDGRYPAHWERFLKLTQPISTRVPYFAVAGNHERTDTEDGVENWRIGTGLPVSGD
                     RLYYSFDSADGWVRFIALDSNPMTDPLNHWSREVEVKYSDEQIDYLQKALSTHRGPSF
                     VFMHHPPFSSGFHRDEWQNDNVLRERRERMVRTMKESGISVLATGHEHAYERALFTWD
                     DAVLISLVAGGAGSPLHAIPSNEQAAKMYSEYKVAGSVVKPENVFTSINFHFIHVRLW
                     FGGGEFTTYAVEKDASTRIIDHVEIDLKRFGVPKIDQFKIPIPEEGPKQGLPVEESKD
                     AATKTANSDSTKADEKLKTEPPPGGKPPR"
     gene            complement(5430..7079)
                     /locus_tag="VF720_12400"
     CDS             complement(5430..7079)
                     /locus_tag="VF720_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880206.1"
                     /translation="MRARGLVYATALLTLNAGSVLAAGSFPDSSGSIRPDTWTDHDGR
                     PILEPGFHEPNYWGHQFREGFVEPLSHSFDIPDQLLDISEAFGANPDRQAVNVNVYDE
                     VPNSSWFTNRNHVRAVSLAEIRKGPGEPIEPARPWTVTKAKKAGRTRGFQIKDADGKK
                     WLVKLDPRGFPQIGSGADLIARTLLHAAGYNVPHNVPVAFSRKELTVDPKLMEAKEDK
                     FGEAELDSLLAGGATLTDGRYSAFASLFIPGSVVGPPSMRHRRKDDPNDRFSHQHRRE
                     LRGLRVLCAWLNSWDMKDQNFLDVFVETADTLGHVDHYVLDVGAALGAAAAGGKKPWN
                     GFEHTVDFKWSMRRLVSLGFVQEPWRKADQETGIPSVGNFESEVYMPHNFRTLVPNAA
                     FREMTDRDGYWGAKLVASFSNAQIQAAVEAVGYEDPRATDYLIRTLIHRRDKTARYYF
                     DRVAPLDFFEIRGGALHFHDLAVDVGLSEPRTYEARISPSQGEGRRLNLGGPVLLLDN
                     LGPGAERLELELSITGSRAASTRVQLTRKGTEWTITRVRHG"
     gene            complement(7079..>7511)
                     /locus_tag="VF720_12405"
     CDS             complement(7079..>7511)
                     /locus_tag="VF720_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880207.1"
                     /translation="YNMWMLEGRAYLPVFASRRVFVMRAAWVGVDPSDESKEFPFYRL
                     VSSERSFRFPGFSTQRFRDRQLMLGQLEYRWLVSHRLGAFLLYQAGAVAPDVNDFHFG
                     DTHISYGGGLRMGRAESEAIRLEVANSVEGVHATLRFRTDF"
BASE COUNT         1265 a         2410 c         2498 g         1338 t
ORIGIN      
        1 cgaggcggtc cgacgtcgac gagacgacgg tgccggtgac cgtgagctgg ctctgggcga
       61 acaccggccc ggccaggacc agcagcaacg tggcaagcag cgcaagcttc ttcattggaa
      121 cctcccttgg agttggggcc agtgtcattt ccccgttggt cggggcccga agggggtccc
      181 cgcgattgtt gggctgcccg gactcagtgc aaactggggg ccaatcgatc gacgcccgaa
      241 agtcgttgaa aaatcagggg tgacggaaat cgggaatcat cgtcacgcgc aaattgttcc
      301 gatacgccgg gacgaaattc cgggtcggac ccattcgatg cgtcggggtt atccccatgc
      361 aacccggcac tggggtgtgt cacgctggaa cacatgcgtc gacgacatgt catctgcctg
      421 ctgagtctcc tcgttccgac cgcggccccg gccgccagcc ccggcgtcgg ctcgatccag
      481 cttttccgac ccctcaccgc cgatcctcgc gaaaaccagt tccgcatggc cttctcggac
      541 tacacggaga actggcgcta cggcactgat atcactgatt ccacgagcca gggaggattc
      601 gaggagcgga gcggcacgcg ctgggacgtg ggaatcggcg ccacgctgcg gctcgacccg
      661 atgcggagga tcttcggctg gaaggggccg tggcatcgat accagctggg attgccggcc
      721 ggcgtcttcg cccggttcga caacggcacg aaggagctta tcgacaccga cttccagttc
      781 ggcgggtcgc tcgacatgct ctgggcggga gagtgggccg acagcacggg ggtgacgacg
      841 ttcgacgggc cggtcgtcac ctcgaggatc accctgttcc atcgcagcac gcacctgggc
      901 gatgaatacc tggcctccgg cgacttcggc gacaaccagg aaggccatcc cgatgaggga
      961 tcgctgtttc atcatccgcc ggtcaagcgc gtcgacctca cgtacgaggc ggtgcgggcc
     1021 atcgtgtcga ccgagtgggc gcccggcttc atcaacgggg gccgttccac gatccgcctg
     1081 tacggcgggg gggagttcaa ggtggcactc cggccgcgga ctccggcgga cttttcctca
     1141 cccatcgccc aggccggggt ggagctacgt tccgccggca accaggacga cccgtcggac
     1201 ggctggatga cgcggctgct caacgcgccg ttcaggcggc cgtttttcga aactgaatgg
     1261 ttcctggcct tcgactggaa gctggcgaag ccgtacgact ttgcctcgtg cgacaacccg
     1321 gacgggagcg gcgaggtgtg gacgccccac ctctggaccg actgccgcca cggccacgag
     1381 ttcgaggggt atgccggctc gtggcacggg atggtggggc tttccctgta cccgggcacg
     1441 gggcggcgcg tggaggaagg ggggcggcgc cagtcgcccg agatcatgct ggcgctggag
     1501 tggtaccgcg gctacgcacc cgacggtcag ttcctcgatc agcgactgga ggcgcatccg
     1561 ttggcatacg tcttgcccgg aatcaccatt cacttctaga acgcagcggc gccgcgcgcc
     1621 gttcgacgac aggaaaaggg gaatcgagac atgagcaagc tattgcgggg cctcgttgcc
     1681 ggctacggcg ccaagaaact cggaggcgga tgcttcagca ccatcatcgt cttcctcctc
     1741 ctctggtggc tcctgggtca cttcaacgtg ttccggtaga cgcccactgg gccatcttcg
     1801 cgatttcctg ggtctgcacg tcggcgatct gctgcgccag cgccttgagc tcggcgtgct
     1861 gcagcttctc gagccccgac gtggccattt tgaccccgcc ctgatggtgc gaagtcatca
     1921 tctcgatgaa tttcgcgtca aaatcggtcc ccgacaatcc ctcgagcccg cccgttccca
     1981 tccgctccat gcccggcatg tcgccgccgg cattcgagtg gccggagtac cactggtcac
     2041 gccacccctt catcagctcg atctgccgtt cctggtcggt gatgaccttg gcggcgaatt
     2101 cctggatctc ggcgcgggaa gcctgcgagg ccgccaggcg ggccatggcc acggcgtcct
     2161 tgtggtggcg gctcatcgtg tcgatgaacc ggaggtcata gtcggcggcc ggctcggccg
     2221 gcttctcggg cgccggcttt tcaaccggcg gaggcttcgg aacctcggcg gacgaggtgc
     2281 ccgaacggac gtccgaaccc gcatcattgc gcgagcagcc ggtggagaga atcgcggacg
     2341 acaggatcag tgcggccagg ctggcaacca gtgcgtgacg gcgatgcatc gtgtggctcc
     2401 ctatcggcac cgttccgtcg gccgcggtgg ccggcgccgg caatgcctca atccgatttg
     2461 gcgaggcgtt tgaagctgcg accgagcgcg aaggccaggc ccagccagac aagtacgagc
     2521 gcggcgttga cgagcgcgaa ctgcttcgaa gcgaagttga gccaggtggt gccggcgtac
     2581 acgagtcctg ccgacaggac atcccccgcc cggacgaaga aggtgtcgtt ggcctgtttg
     2641 gccttgtact tggcctcccg gctggtgggc aggtagagcg cgtttcgcgt ggtgttctgg
     2701 agcgagtaat cgagcgagtt ctcggccatc ttcacgccgc ggatgaacga gagcaccggg
     2761 atgaaggcca tggcggcata gccggcaaag gccaccaccg gcatcaccat gagggccgtc
     2821 cgcacgccga agtacttgat gacgcgcgac accacgaagg cctgcagcag cgccgagatg
     2881 atgttgacga tggtgaagta gttgccgtag aactcgccga tcagcttttt ctcatcggcg
     2941 ccgcccccct ggccgtggct gaggaggtcc acaacggtct tgccgagaat gtactcgccg
     3001 gtggtgttga ccaggttcac caccagcatg agaaggccga tcatcaggag gtaacggtcc
     3061 gacaggacga gcgcgaatcc gctcttgccg ccgatcgtct cgccgacatc ctccttcttc
     3121 gccgggtctc ccgggctctt ccccgccgcg tgggcctttt cccgcatgtg gatcaggttg
     3181 gtcaggaaca tgcacacgcc gagcagcacc gccgcggcca gcatgaaagg gtaggggccg
     3241 tactgcttga ccagctgccc ggtggcgaac gagccggcaa tggccccgag cgaggcgccg
     3301 acgcccacga tggcaaagag gcgcttcccc tgttccacgg tgtacacgtc gttcgcgaag
     3361 gcccagagct gggcgatcat catcacgttg aagatgccga cccagatgaa gaacggcact
     3421 cccaccgcca ctcccgcccg gttcaacacg aagaaagcca ccaggcaggc gatgaagatg
     3481 agtgtgacgc cgttgatcag cttgacgcgc gacacgcgcg aggccagcgc gctgtaggcc
     3541 gggaccagga tgaccaggag gcccgcgatg gcggcggcgg cgtaactctt gatctccgca
     3601 ccgccgttgc cggccaggat gagcggctcg cggatcgtct tgagcaggta gtagctggcc
     3661 aggagcagga agatgttgaa catgaggagg agcgcggtga atccctcgcc ccccttcacg
     3721 tcggcaaaga tgccgaggaa ccggtcgagc ggccccttgt ggccggcgtc ggtacgcgcc
     3781 ggtggcgcgg tgtcgtcgtt ccttcgattc atcgtgtctc ctcgggcacg accgctcctg
     3841 tctggcgcgg cctcagcggg gcggcttccc gccgggcggt ggttcggtct tgagcttctc
     3901 gtcggctttc gtcgagtcgg agttggccgt cttggtggca gcgtccttcg actcttccac
     3961 cggcagcccc tgtttcggcc cctcctcggg gatcgggatc ttgaactggt cgatcttcgg
     4021 cacgccgaat cgcttgaggt cgatctccac gtggtcgatg atccgggtgc tggcatcctt
     4081 ctccacggcg taggtcgtga actccccgcc gccgaaccat aaccgcacgt ggatgaaatg
     4141 gaaattgatg gacgtgaaga cattttccgg cttcacgacc gacccggcca ccttgtactc
     4201 ggagtacatc ttcgcggcct gctcgttgga ggggatggcg tggagcggcg agccggcgcc
     4261 gcccgccacc aggctgatca ggacggcgtc gtcccaggtg aacagggccc gctcgtacgc
     4321 atgctcgtgg ccggtggcca gcaccgagat ccccgattcc ttcatcgtgc gcaccatccg
     4381 ttcacgacgt tcgcgcagga cattgtcgtt ctgccactcg tcgcggtgaa acccgctcga
     4441 gaagggtgga tggtgcatga agacgaacga cgggccccgg tgtgtcgaga gggccttctg
     4501 caggtagtcg atctgctcgt ccgagtactt gacctccacc tcgcgcgacc agtgattgag
     4561 cggatcagtc atcgggttcg agtcgagggc gatgaagcgc acccagccgt ccgccgagtc
     4621 gaaggagtaa tagagtcgat cgccgctcac cggcaggccg gtgccgatgc gccagttctc
     4681 cacgccgtcc tcggtgtcgg tgcgttcatg gtttcccgcc acggcaaagt agggcacccg
     4741 cgtcgagatc ggctgggtga gcttgaggaa acgctcccaa tgcgccgggt agcggccatc
     4801 cttcaccagg tctcccgtgt tgatgatggc cgccaccgtt ttcttccggg cctcgaggga
     4861 atcgaccagc accccgatgg ccttgagaac ctgctcctcg cgcccgtaac tgggcttgtt
     4921 ggtgatcagg ttggggatgt cgcggatgag cgccaggtcg ggatagagcc ccttgaagag
     4981 gaagacgggg agcgtgatca gccccttcac ccacttcacc gggttcaggg accacattgc
     5041 cttgagcctg gtggtctccg gcttcagacg ggcggtgcgc caggagggac ggttgtcgcc
     5101 gaacaggatg atgttgagcg tatcggccgt gtagccggtg cgcagccgcc ccaggaagcg
     5161 ggcggacgtg tcctcggtgc ccggcaccag gccgatgagc gtgtcgctga agagcggagt
     5221 ccagggcgca acggggccga cgaatgcctt ggtttcggtg gcgccggccg agtcggtcac
     5281 gacgggggac accgtctcct gcaccggggc caaccgcccc attcgggcgc acccggcaat
     5341 gctgagcgtg atcacgatcg cggtgatgac gattcgttcg gccgacggaa ctgttcggtc
     5401 ccttcgccgc ctgccgccca ctcccttcgt catccgtgtc gaacccgggt gatggtccat
     5461 tccgttccct ttcgagtcag ttgcacccgg gttgatgccg cccggcttcc ggtgatcgat
     5521 agttcgagct ccaggcgttc cgcgccagga cccaggttat ccagcaagag taccggtccg
     5581 ccgaggttca ggcgacgacc ttccccctgg ctcggggaaa tgcgcgcttc gtacgtgcgc
     5641 ggttcggaga gccccacgtc gacggccagg tcgtggaagt gcagggcccc cccccggatc
     5701 tcgaagaagt cgaggggcgc cacccgatca aagtagtagc gggccgtctt gtccctccgg
     5761 tggatgagcg tgcggatcag gtagtcggtg gcgcgcggat cctcgtaccc gaccgcctcc
     5821 acggctgcct ggatctgtgc gttcgagaac gaggcaacca gcttggcgcc ccagtagccg
     5881 tcgcggtcgg tcatctcgcg gaaagccgcg ttcggcacca gggtccggaa gttgtgcggc
     5941 atgtacacct ccgactcgaa attgccgacc gatgggatgc cggtctcctg gtcggccttc
     6001 cgccagggtt cctgcacgaa gccgagcgac accagccggc gcatcgacca cttgaagtcc
     6061 accgtgtgct cgaagccgtt ccatggtttt ttccccccgg ccgccgcggc gccgagcgcg
     6121 gcgcccacgt cgagcacgta atgatccacg tgccccagcg tgtcggcggt ctcgacgaat
     6181 acgtccagga aattctggtc cttcatgtcc cagctgttca gccaggcgca gagaacccgg
     6241 aggccccgga gctcccgccg gtgctggtgg ctgaagcgat cgttgggatc gtccttgcga
     6301 cgatgccgca tgctgggagg gcccaccacg gaacccggga tgaagaggct ggcgaaggcc
     6361 gagtagcggc cgtccgtcag cgtggcgccg cccgccagga gcgagtcgag ctccgcctcg
     6421 ccgaacttgt cctccttggc ctccatgagc ttggggtcga ccgtgagctc cttcctcgag
     6481 aaggccaccg gcacgttgtg gggcacgttg taccccgccg cgtgcagcag cgtccgtgcg
     6541 atgaggtcgg ctcccgagcc gatctgcgga aaccccctgg gatcgagctt caccagccac
     6601 ttcttgccgt cggcgtcctt gatctggaat ccgcgcgtcc gcccggcctt cttggccttg
     6661 gtcaccgtcc atggccgcgc gggctcgatg ggctcgcccg gtcccttccg gatttcagcc
     6721 agggagaccg cccggacgtg gttccggttg gtgaaccagc tcgagttcgg cacctcgtcg
     6781 tacacgttga cattgacggc ctgtcggtcc gggttggccc cgaacgcctc gctgatgtcg
     6841 aggagctggt cggggatgtc gaacgaatgg gacaacggct cgacgaatcc ctcccggaac
     6901 tggtgtcccc agtagttggg ctcgtgaaaa cccggttcca ggatgggccg gccgtcgtgg
     6961 tcggtccagg tgtccggtcg gatcgaaccg gaggaatccg gaaagctgcc ggccgcaagg
     7021 acggaaccgg cattcagggt caggagcgca gtcgcgtaaa ccagtccacg ggcgcgcatc
     7081 agaagtccgt ccggaagcga agggtggcgt gcacgccttc gacgctgttg gcaacctcga
     7141 ggcggatggc ttccgactcg gcgcgaccca ttcgaaggcc gcccccatag gaaatgtgcg
     7201 tgtcgccaaa gtggaagtcg ttgacatcgg gagccaccgc tccggcctgg tagagcagga
     7261 aggccccgag gcggtgcgaa accagccacc ggtactcgag ctgccccagc atgagctggc
     7321 gatcgcggaa tcgctgggtc gagaagcccg gaaagcggaa gctccgttcg ctcgacacga
     7381 gccggtagaa cgggaactcc ttgctttcgt cggagggatc gactcccacc catgcggccc
     7441 gcatcacgaa gacgcgccgg ctggcgaaga cgggcaggta agcgcgcccc tcgagcatcc
     7501 acatgttgta g
//
LOCUS       DASVOD010000561         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_766458, whole genome shotgun sequence.
ACCESSION   DASVOD010000561 DASVOD010000000
VERSION     DASVOD010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766458"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF720_12410"
     CDS             <1..379
                     /locus_tag="VF720_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX7880208.1"
                     /translation="SLTLVGLAGSPMLDVWRTMGFTVAGEAFADRRYESNGRLRSRHH
                     ADALIDDPREAARQAVLLAVEGTVTSIDGSVIPCRADTLCLHGDQPGALERVLTVRAA
                     LEAAGVAVRSLPGISGLPAPPVT"
     gene            complement(348..1556)
                     /locus_tag="VF720_12415"
     CDS             complement(348..1556)
                     /locus_tag="VF720_12415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX7880209.1"
                     /translation="MHPWVFMVLIVPFGVVSGFVSVALAFHLTRAGVSVGQVAGIVAL
                     VVLPHTWKFLWAPIIDATLTQKAWYWIGAVLTAVGIAVMGALPPTAAGLAFLSGVVFV
                     TSSATSFIGMAVESLMAHATPEHEKGRAGGWFQAGNLGGSGIGGGLGLWLAQHLSSAW
                     LAGAIVAVLCFLCSLALFLVPTPSRSHATDGFWKALRDAITDLWVVIREKSGTLALIL
                     CFLPIGSGAASGLWSAVAGEWNAGPDTVALVTGILAGVISAVGCVIGGWICDRMDRRL
                     AYLGFGLLQAGAAVALALLPRTPTSFVVLTSLYALITGFTYAGFTAFVLEAIGKGAAA
                     TKYSVYASLSNMPIYYMTWIEGAAHDRLGSGGMLLTEAVLGTAGAVVFVALMFGLGAR
                     FKSRAALAIR"
     gene            complement(1594..2127)
                     /gene="msrA"
                     /locus_tag="VF720_12420"
     CDS             complement(1594..2127)
                     /gene="msrA"
                     /locus_tag="VF720_12420"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017731886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX7880210.1"
                     /translation="MSERITLGGGCFWCIEAVLKDLKGVEKVESGYAGGHVRNPGYRE
                     VCEGTTGHAEVVDVTFDPSVVSLESLLEVFFTLHDPTTLNRQGHDSGTQYRSIILYRD
                     EEQKRVAEGVRADFERRGVWGRPIVTTIEPLDTFWKAEASHQDYYARNGSQPYCQAVI
                     EPKVSKLRKEFFDRLKS"
     gene            2213..3748
                     /locus_tag="VF720_12425"
     CDS             2213..3748
                     /locus_tag="VF720_12425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX7880211.1"
                     /translation="MSDTTLPLFSGRLADPQEVLEQVFGFTEFRPGQKRIIDAVLAGH
                     DAIGVMPTGAGKSLTFQVPARILPGTVLVVSPLISLMKDQVDAMVRYGFSAAVLNSTL
                     DFDARREVLGRLRRGELHLLYVAPEGIEGSLRSLIAECDISLVVVDEAHCISHWGHEF
                     RPAYRKLKGLKGEMGNIPVLALTATATRKVAADIVAQLGMTKPDGFRGSFYRPNLHVT
                     AQKKGEGRNTRKDILGIIRKHPGESGIVYCLSRKGVDQLTTWLVQQGVKALPYHAGLP
                     DEERRRSQDAFARDEADVIVATVAFGMGIDKSNIRFVIHRDMPKSVEAWYQEMGRAGR
                     DGLPSDCVLMYSWSDVIGYDTFLDNIDNDTLREETRKKTVDMFRLVDSGGCRHQKLVA
                     CFEEAIEPCGESCDVCRGITVGDIVAKVWVVEKKSGSKSPAASFTADPELFESLRALR
                     RRIADQENVPAYIVFSDAVLTALSDRKPKTPDEMLTVPGVGPAKLARYGREFLELINS
                     PGA"
     gene            complement(3785..4360)
                     /locus_tag="VF720_12430"
     CDS             complement(3785..4360)
                     /locus_tag="VF720_12430"
                     /inference="COORDINATES: protein motif:HMM:NF039391.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HEX7880212.1"
                     /translation="MMARRMDQYGGESGQSTLFRRRCRRSLPVLMLASAWLAGVALPS
                     SAAEPPKAAPAPATIEDLGWLTGSWSGESNGMRMEEHWTDAGGGGLVGMHKDVKAGKM
                     VSFEFFRIGPDKGGKVTYFTQPGGVPAIPFVAIEMSSSRVVFENAAHDFPQRILYWAG
                     DDGRLHARIEGMMNGKTASEEWSWARMTAAR"
BASE COUNT          789 a         1560 c         1412 g          692 t
ORIGIN      
        1 cagcctcacg ctggtcggcc tcgccggttc gccgatgctc gatgtgtggc gcaccatggg
       61 gttcaccgtg gcgggcgaag ccttcgccga ccggcgctac gaatcgaacg ggcggctccg
      121 ctccaggcac cacgccgacg ccctgatcga cgatccgcgt gaagccgccc gccaggccgt
      181 actgctggca gtggagggga ccgtgacgtc gatcgacggc agcgtgatcc cctgccgcgc
      241 cgacacgctc tgtctgcatg gtgaccagcc gggcgcgctc gaacgggtgt tgacggtgcg
      301 cgcggcgctc gaggcggcgg gggttgccgt ccgaagcctc cccggtatca gcggattgcc
      361 agcgccgccc gtgacttgaa tcgcgccccg agcccgaaca tcagcgccac gaacaccacc
      421 gcgccggccg tgccgagcac ggcttcggtg aggagcatcc cgccggaacc caaacggtcg
      481 tgggccgcgc cctcgatcca ggtcatgtag tagatgggca tgttggagag ggaggcgtac
      541 acgctgtact tggtggccgc cgctcccttg ccgatggcct ccagcacgaa ggcggtgaag
      601 cccgcgtagg tgaatccggt gatgagggcg tacaggctcg tgagcaccac gaacgatgtc
      661 ggcgtgcgag gcaggagcgc cagcgccacg gcggcccccg cctgcaggag tccgaagcca
      721 agatacgcca gccggcgatc catgcgatcg cagatccagc cgccgatcac gcatccgacg
      781 gccgagatca cgccggccag gatgccggtg acgagcgcca cggtgtcggg gccggcgttc
      841 cactcaccgg ccacggccga ccagaggccg ctcgccgccc ccgatccgat cggcaggaag
      901 cagaggatga gcgccagcgt gcccgacttc tcccgaatca cgacccagag atcggtgatc
      961 gcgtcgcgga gggccttcca gaagccgtcg gtcgcgtgcg aacggctggg cgtcggcacg
     1021 aggaaaaggg ccaggctgca gaggaaacag agcaccgcca cgatggcccc cgccagccag
     1081 gccgacgaca ggtgctgcgc cagccagagg ccgagcccgc cgccgatgcc gctccccccc
     1141 aggttgcccg cctggaacca ccccccggcg cgacctttct cgtgttcggg cgtggcgtgg
     1201 gccatgagac tctccaccgc catgccgatg aacgaggtgg ccgagctggt cacgaacacc
     1261 acgcccgaca ggaacgcgag gccggccgcc gtcggtggaa gcgcgcccat gaccgcgatg
     1321 ccgaccgcgg tgaggacggc gccgatccag taccaggctt tctgcgtgag cgtggcgtcg
     1381 atgatcggcg cccagaggaa tttccaggtg tgcggcagga ccacgagcgc cacgatgccg
     1441 gcaacctgcc ccacggagac gccggcccgg gtgagatgaa acgccagcgc cacgctcacg
     1501 aagccggaca ccacgccgaa cgggacgatg agcaccatga acacccaggg gtgcataccc
     1561 ccgcgtgggt gcgttgcgcc gtcccccgcc gcctcaggac ttgagtcgat cgaaaaactc
     1621 cttccgcaac ttcgaaacct tcggctcgat cacggcctgg cagtagggct ggctgccgtt
     1681 ccgggcatag taatcctggt ggctcgcttc cgccttccag aacgtgtcga gcggctcgat
     1741 ggtagtcacg atcggccgcc cccacacccc ccgcctctcg aagtcggccc tgacgccctc
     1801 ggccacgcgt ttctgctcct catcgcgata caggatgatc gagcggtatt gcgtaccgga
     1861 atcgtgcccc tggcggttca gcgtggtggg gtcgtgaagg gtgaagaaaa cctccaacag
     1921 gctctcgagc gacacgaccg acgggtcgaa cgtgacgtcc acgacctcgg catggccggt
     1981 cgtcccctcg cacacctccc ggtagccggg attccggaca tggccgccgg cgtaacccga
     2041 ctcgactttt tccacaccct tcagatcctt caacacggct tcgatgcacc agaagcaacc
     2101 gcccccgagc gtgattcgtt cactcatcgt ttgtctcccg tggttcgttc caagtagagt
     2161 ctaccccgat tcgttccgcg cccatgcacc cagccgcccg tgtggagatt ccttgtccga
     2221 tacaacgctg ccgctttttt ccggccgcct tgccgacccg caggaagtcc tcgagcaggt
     2281 gttcggcttc accgagttcc gcccgggcca gaagcgcatc atcgacgccg tcctcgccgg
     2341 acacgacgcc atcggtgtca tgcccaccgg cgccggcaag tcgctcacgt tccaggtgcc
     2401 ggcccgcatc ctgccgggca ccgtgctggt cgtctcgcca ctcatctcgc tgatgaagga
     2461 ccaggtggat gccatggtgc gctacggatt ctcggccgcg gtgctcaatt ccaccttgga
     2521 tttcgacgcg cgcagggagg ttctgggccg cctgcgtcgc ggcgagctgc atctcctcta
     2581 tgtggcgccg gaagggatcg aggggtcgct ccgttccctg atcgcggagt gcgacatctc
     2641 cctggtggtc gtggacgagg cccactgcat ctcccactgg ggccacgagt tccggccggc
     2701 ttaccgcaag ctgaaggggc tcaaggggga gatggggaac attcccgtcc tggcgctcac
     2761 cgccaccgcc acccgcaagg tggccgccga catcgtggcc cagctcggga tgacgaagcc
     2821 cgacggcttt cgcggctcat tctaccgccc caacctccat gtaaccgcac agaagaaggg
     2881 agaagggcgc aacacgcgca aggacatcct ggggatcatc cgcaaacacc ccggcgagag
     2941 cggcatcgtc tactgcctga gccgcaaggg agtggaccag ctgaccacgt ggctcgtgca
     3001 gcagggcgtg aaggcgctgc cgtaccacgc cggcctgccg gacgaggagc gccgccgcag
     3061 ccaggacgcg tttgcccgcg acgaggccga tgtcatcgtg gccaccgttg cgttcggcat
     3121 ggggatcgac aagagcaaca tccgcttcgt catccaccgc gacatgccca agagcgtgga
     3181 agcctggtac caggagatgg ggcgcgccgg ccgcgacggc ctgccgagcg actgcgtcct
     3241 catgtactca tggtccgatg tgatcggcta cgacacgttc ctcgacaaca tcgacaacga
     3301 tacgctccgc gaggaaacgc ggaagaaaac cgtggacatg ttccgcctgg tcgacagcgg
     3361 cggctgtcgc caccagaagc tggtggcctg cttcgaggaa gccatcgagc cgtgcggcga
     3421 atcgtgcgat gtttgccgcg gcatcaccgt cggcgacatc gtggcgaagg tatgggtggt
     3481 cgagaagaag agcggctcaa agagccccgc cgccagcttc acggcggatc ccgaactctt
     3541 cgagagcctg cgcgcgctgc gccgccgcat cgccgaccag gagaatgtgc cggcttatat
     3601 cgtgttcagt gacgcggtgc tgacggcgct ctccgaccgc aagccgaaga caccggatga
     3661 gatgctcacg gtgccgggcg tgggccctgc caagctggcg cgctacggca gggagtttct
     3721 cgaactgatc aactcgccgg gggcctgacg cccgcgactg tcaccggttt aagtcgccac
     3781 cgggtcaccg ggccgcggtc atccgcgccc aactccactc ttccgaggcc gtcttcccgt
     3841 tcatcatccc ctcgatccgg gcatgcagcc ttccgtcgtc gccggcccag tagaggatcc
     3901 gctgcgggaa gtcatgcgcg gcgttctcga acaccacgcg cgacgacgac atctcgatcg
     3961 ccacgaacgg tatcgccgga accccacccg gctgcgtgaa gtaggtgacc ttccccccct
     4021 tgtcgggccc gatccggaag aactcgaacg acaccatctt ccccgccttc acgtccttgt
     4081 gcatgccgac aaggccgccg ccacccgcat ccgtccagtg ctcttccata cgcatgccgt
     4141 tcgactcacc ggaccaggag ccggtaagcc agccgaggtc ctcgatggtg gccggcgcgg
     4201 gggccgcctt gggcggttcg gcagccgatg atggcagggc tacccctgcg agccaggcgc
     4261 ttgccagcat gagaacaggt aatgaccggc ggcaacgccg ccggaacaaa gtcgactgac
     4321 ccgactcacc gccgtactga tccatccgcc tcgccatcat cgttacctcc agtctcaagg
     4381 cgacatgagc gtcgccagtc atgacagtca cggtatcgaa caatcagtcg cccccgcttg
     4441 gacaaatgcg tca
//
LOCUS       DASVOD010000562         1649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_506662, whole genome shotgun sequence.
ACCESSION   DASVOD010000562 DASVOD010000000
VERSION     DASVOD010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506662"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1649
                     /locus_tag="VF720_12435"
     CDS             <1..>1649
                     /locus_tag="VF720_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="HEX7880213.1"
                     /translation="TITAFDAGGGDIAVDYEILNRTTRSIRAGLMLAARPYQVNPPAQ
                     FLNLAGGTARIRKIERHGTQINGTLVVNDRQMFIGHGELIAAPYESGDVVADWLRTGR
                     LPATSAAYDTFEAASGLVNIDELTLSPGSMSMFRLTIPFKDGSTPHRSDDLGKTIEKW
                     RRILHVPRIEVPTAGQPLIESMNAQLGYILVNRAGPAIQPGSRAYARSWIRDGSLTSS
                     ALLRLGHFKEVADFIKWFAPHQFENGKIPCVVDKRGADPVPEHDSSGEFIFLVAEYLR
                     YTGDRSLAREMWPRVERAAVYLDTLRQERRTDAWRAPDKAEFFGLLPPSISHEGYSAK
                     PMHSYWDDSFAYRGFKDAAWLAGQLGKKREAANWDVVAAEFGRDLGNSVAAARRRHGI
                     AYIPGCADLGDFDATSTTIFLSPTGASTVVTPDELTATFDKYETFFTERAAGAPWEAF
                     TPYEIRNIGAFVRLDRRDRALAMAEWSLGTQQPEGWRQWPEVVWHPGTPQKFIGDLPH
                     TWVGSDFIRSVLDMFVYVREDDQALVVGAGIPTAWLDDPGGV"
BASE COUNT          273 a          591 c          523 g          262 t
ORIGIN      
        1 accatcaccg cctttgacgc gggcggcggc gacatcgccg tcgactacga gatcctgaac
       61 cggacgaccc gctcgatccg cgccggcctc atgctcgcgg cgcgccccta ccaggtcaat
      121 ccaccggccc agttcctgaa cctggccggc ggtaccgccc gcatccgcaa gatcgagcga
      181 cacggcaccc agatcaacgg gacgctcgtc gtcaacgatc gtcagatgtt catcgggcac
      241 ggcgaactca tcgcggcgcc gtacgagtcg ggcgatgtgg tggccgactg gctccggacc
      301 ggcaggctac cggccacttc cgccgcgtac gacaccttcg aggcggcctc ggggctcgtg
      361 aacatcgatg agctgacgct ctcgcccggc agcatgtcga tgttccgcct caccattccg
      421 ttcaaggatg gttccacgcc ccaccggagc gacgacctcg gcaagaccat cgaaaaatgg
      481 cggcgcattc tccacgtgcc gcgcatcgag gttcccacgg ccggccagcc gctcatcgaa
      541 tcgatgaacg cccagctcgg atacatcctg gtgaaccggg ccggccccgc catccagccc
      601 ggcagccgcg cctacgcgcg ctcctggatc cgcgacggct cgctcacctc ctcggccctc
      661 ctgcgcctcg ggcatttcaa ggaagtggcc gacttcatca agtggttcgc gccccaccag
      721 ttcgagaacg gcaagatccc ctgcgtggtg gacaagcggg gcgccgaccc ggtaccggag
      781 cacgactcga gcggggagtt catcttcctc gtggccgagt atctccgcta cacgggcgat
      841 cgttcactgg cgagggagat gtggccgcgc gtcgaacggg cggcggtcta cctcgatacg
      901 ctgcgacagg agcggcgcac cgacgcatgg cgcgcgcccg acaaggcgga gttcttcggc
      961 ctcctcccgc catcgatcag ccacgaaggg tactcggcca agccgatgca ctcctactgg
     1021 gatgactcgt tcgcttaccg cggcttcaag gacgccgcct ggctggccgg ccagctggga
     1081 aagaaacgcg aagcggcgaa ctgggacgtt gtggcggcgg aatttggtcg cgaccttgga
     1141 aactcggtgg ccgcggctcg ccggcgccac ggcatcgcct acatccccgg atgcgccgac
     1201 ctgggggatt tcgacgccac gtccacgacg atctttctct cccccaccgg cgcttcgacg
     1261 gtggtcacac cggacgagct caccgccacg ttcgacaagt acgagacgtt cttcaccgaa
     1321 cgggcggccg gcgcgccgtg ggaagccttc accccgtacg agatccggaa catcggggcc
     1381 ttcgtgcggc ttgaccgccg ggaccgcgcc cttgccatgg ccgagtggtc cctcggcacg
     1441 cagcagccgg aagggtggcg gcagtggccc gaggtggtct ggcatccggg cacgccgcag
     1501 aagttcatcg gcgacctccc tcacacctgg gtcggctcgg acttcatccg ttccgtgctc
     1561 gacatgttcg tctacgtccg ggaggacgac caggcgctgg tcgtcggggc cggtattccc
     1621 acggcatggc tcgacgatcc cggcggcgt
//
LOCUS       DASVOD010000563         3916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_713708, whole genome shotgun sequence.
ACCESSION   DASVOD010000563 DASVOD010000000
VERSION     DASVOD010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713708"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /gene="tssH"
                     /locus_tag="VF720_12440"
     CDS             <1..1794
                     /gene="tssH"
                     /locus_tag="VF720_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019622385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEX7880214.1"
                     /translation="ENRLKNVINEVKSSPKPIILFIDEAHTLIGAGGAAGGGDAANLL
                     KPALARGELRTIAATTWSEYKKYFEKDPALARRFQPVKLDEPSEEDTITMLRGLREKY
                     EESHKVRILDEAVVAAAKLSARYISGRQLPDKAVDLLDTSAARVKIGLSSKPASLEDV
                     EGRIRTMEREQAAQTRDQKAGKTIDAEYAAELEAKLVAARAEQEALTARWKTEQEAAN
                     AVVKLREEIEKLPPAEQDAKKPEIDQVLAKLTEVQANDPLIHIDVNPELVGHVVSDWT
                     GIPLGKMVRDEAAQILAFDGQLKQRIKGQDHAIDAIAKGMRAAKAGIGNPRAPMGIFL
                     FVGPSGVGKTETSLGVADLLFGGERFMTTINMSEFQEKHTVSRLIGSPPGYVGYGEGG
                     MLTEAVRQRPYSVVLLDEVEKADPEVMNLFYQVFDKGMLSDGEGREVDFKNTVIFLTS
                     NLASDVIMGMAQGPVRPDPEELTSAIRPALNKHFKPALVARMTIVPFYPISVDAMKDI
                     VKLKIGAIGQRLAKSHRMAFDVDPAVIDQVAQRCTEVETGARNIDHILSGSLLPKIST
                     EILTRMNEGALPKRLKLGLDSGGEFELSFSD"
     gene            complement(1821..2351)
                     /locus_tag="VF720_12445"
     CDS             complement(1821..2351)
                     /locus_tag="VF720_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX7880215.1"
                     /translation="MARLVFGMNQSLDGYVDHTEFGPSPTLFRHFIEEAKQQAGSIYG
                     STMYGLMRYWDDDQPDWDEPRREFAAAWRRQPKWVVSRSSPSVGPNATLVTGDLATAI
                     RRIKAEHDGEIEVAGPNLAKTLTALGLIDEYRIYLHPVVLGRGAPYFAGPRPPLRLVA
                     HDRIDEDVIRLTYEPA"
     gene            complement(2390..2803)
                     /locus_tag="VF720_12450"
     CDS             complement(2390..2803)
                     /locus_tag="VF720_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009981804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like domain-containing protein"
                     /protein_id="HEX7880216.1"
                     /translation="MFPASCKSSGMCMTTGPTDVCKTPAPPAPVPPPIPYPNMASCGQ
                     ASGCADKVKICGSSAITTKSDIPMSSGDEPGVAGGMVSNVFKQKCSFPMGSGKVKAEG
                     AAVVCLTKMTKHNGSNANTPGMHTVPCQVKVLVAG"
     gene            complement(2818..3381)
                     /locus_tag="VF720_12455"
     CDS             complement(2818..3381)
                     /locus_tag="VF720_12455"
                     /inference="COORDINATES: protein motif:HMM:NF023482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3540 domain-containing protein"
                     /protein_id="HEX7880217.1"
                     /translation="MEAQEHQILTVPNGGQELAPAPGLILGPAVVRGVMDGALLVEHE
                     GSLERATMALAYPYRAVTGDVVLILGQEDRFYVTGVLDGQGMTRLDFPGDAELRAAGT
                     LRLSAAEGVELDSQRISMRAEKLDMTIASIRQQVASFYQHVTGTLRTIAGRQRTHVEK
                     ESTLHARKIVRKAEDDVIVDGRQIKLG"
     gene            complement(3354..>3916)
                     /locus_tag="VF720_12460"
     CDS             complement(3354..>3916)
                     /locus_tag="VF720_12460"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX7880218.1"
                     /translation="TDFSRSSCNKTDLSGIDFSGCKLTQFGLAGADCRGGNFRNTVMT
                     FAELTGGTFDGADFAGAKLDSAECRKGSFNDASFAGADIAGASFEEAELSRANLAGAK
                     ARESRFIKADLRKATATGGDFEYADFSYANLELADFSRTNLSGSTMHLTMKETTNLDG
                     ARTKGMKGTDLDLLEAETWKPKSTKS"
BASE COUNT          682 a         1251 c         1301 g          682 t
ORIGIN      
        1 gagaaccggc tcaagaacgt catcaacgaa gtgaagtcct cgcccaagcc gatcattctt
       61 ttcatcgacg aggcgcatac gttaattggc gccggaggcg ccgcgggggg tggggatgcg
      121 gccaatctgt tgaagcccgc gctcgcgcgc ggggagctgc gcacgatcgc cgccacgacc
      181 tggtccgaat acaagaagta cttcgagaaa gacccggccc tcgcccgtcg tttccagccc
      241 gtcaagctcg acgagccgtc tgaagaagac acgatcacca tgcttcgcgg cctccgcgag
      301 aagtacgagg agtcgcacaa ggtgcggatt ctcgacgagg cggtcgtggc ggcggccaag
      361 ctgtcggcgc gctacatctc gggacggcag ctgccggaca aagcggtcga cctgctggat
      421 acctcggccg cgcgcgtgaa gatcggcctg tcatccaagc cggcatcgct ggaggacgtg
      481 gagggtcgca tccggacgat ggagcgcgag caggccgccc agacgcgcga ccagaaggcc
      541 ggcaagacga tcgacgccga atacgccgcc gagttggagg ccaagctcgt ggccgcccgc
      601 gccgagcagg aggccctcac ggcccgctgg aagaccgagc aggaagccgc caacgccgtc
      661 gtgaagctgc gcgaggagat cgagaaactg ccgccggccg agcaggacgc caagaaaccc
      721 gaaatcgacc aggttctggc gaagctcacg gaggtgcagg ccaacgaccc gctcatccat
      781 atcgacgtga accccgagct cgtggggcac gtggtcagcg actggaccgg cattcccctc
      841 ggcaagatgg tgcgcgacga ggccgcccag atcctggcct tcgacggcca gttgaagcag
      901 cgcatcaagg ggcaggacca cgccatcgac gccatcgcca agggaatgcg cgccgccaag
      961 gccggcatcg gcaacccgcg cgcgccgatg gggatcttcc tgttcgtcgg cccctcgggc
     1021 gtcggcaaga ccgagacgag cctcggcgta gccgacctcc tgttcggcgg cgaacgcttc
     1081 atgacgacga tcaacatgtc ggagtttcag gagaaacata cggtgagtcg actcatcggc
     1141 tcgccgccgg gctacgtggg ctacggcgag ggtggcatgc tcaccgaagc cgtgcgccag
     1201 cgcccctact ccgtggtgct gctcgacgaa gtggagaagg ccgatcccga agtgatgaac
     1261 ctcttctacc aggtctttga caagggaatg ctgtcggacg gcgaaggtcg cgaggtggat
     1321 ttcaagaaca cggtgatctt cctcacctcc aacctggcca gcgacgtcat catgggaatg
     1381 gcccagggcc cggtacggcc cgaccccgag gaactgacca gcgccatccg ccccgcactc
     1441 aacaagcact tcaagccggc gctcgtggcc cgcatgacga tcgtcccgtt ctatcccatc
     1501 tcggtggacg cgatgaagga catcgtgaag ctcaagatcg gggcgatcgg ccagcggttg
     1561 gccaagagcc atcgcatggc gttcgacgtg gatccggcgg tgatcgacca ggtggcgcag
     1621 cggtgtaccg aggtggaaac gggtgcgcgc aacattgatc acatcctgtc cggctcgctc
     1681 ctgccgaaga tctcgacgga gattttgacg cggatgaatg agggggcgct gccgaagcgg
     1741 ttgaagctgg ggttggactc gggtggggag tttgagctct cgttctccga ctagcggccg
     1801 aagggcggtc ggctacactt tcaggccggc tcgtacgtca accggatgac atcctcgtcg
     1861 atccggtcat gggccacgag gcggagcggc ggccgcggtc cggcgaagta gggcgcgcca
     1921 cggcccagca ccaccggatg caggtagatc cgatattcgt cgatcagtcc gagcgcggtc
     1981 agtgtcttcg ccaggttcgg gccggccact tcgatctcgc cgtcgtgctc ggccttgatc
     2041 cgacggatcg ccgtcgcgag gtcgcccgtc accagcgtgg cgttggggcc gaccgacggc
     2101 gatgagcgcg acacgaccca tttcggttgc cgccgccatg cggcggcgaa ttcccgcctc
     2161 ggctcatccc aatctggttg gtcatcgtcc cagtaccgca tgaggccgta catcgtgctg
     2221 ccgtagatgc tcccggcctg ctgcttcgcc tcttcgatga agtggcgaaa gagcgtgggg
     2281 ctcggcccga attccgtgtg gtcgacgtag ccgtcgaggg actggttcat gccgaagacg
     2341 agacgggcca ttgaattcct ttgctcgcga agcgaatccg cgcggtttct taccccgcca
     2401 ccagcacctt cacctggcac ggcaccgtgt gcattcccgg tgtgttggca ttactgccgt
     2461 tgtgcttggt catcttcgtc aggcagacca cggcggcgcc ttcggctttc accttgcccg
     2521 agcccatcgg aaagctgcac ttctgcttga agacgttcga taccattccg ccggcgactc
     2581 cgggttcatc gcccgaagac atggggatat cggacttggt cgtgatggcg gacgagccgc
     2641 agatcttgac cttgtcggcg cagccggagg cctggccgca gctggccatg ttgggatacg
     2701 ggatcggcgg cggcaccggt gcggggggcg ccggcgtctt gcagacgtcg gtggggcccg
     2761 tggtcatgca catgccgctc gacttgcagg atgcgggaaa catggtgcga tctcctctca
     2821 gcccagcttg atctgcctgc cgtcgacgat aacgtcgtcc tcggccttgc ggacgatctt
     2881 ccgcgcgtga agcgtcgatt ccttctcgac gtgcgtgcgc tggcggccgg cgatcgtgcg
     2941 cagggtgccg gtgacgtgct gatagaagct ggccacctgc tggcggatcg acgcaatcgt
     3001 catgtcgagc ttttcagccc gcatgctgat ccgctgcgag tcgagctcca cgccttccgc
     3061 cgcgctcagg cgcaatgtcc cggccgctcg caattccgca tcgccgggaa agtcgagccg
     3121 ggtcatcccc tgcccgtcca ggacgccggt cacgtagaac cgatcctcct ggccaaggat
     3181 gaggacgaca tcgccggtaa cggcccggta cgggtaggcc agcgccatcg tggcccgctc
     3241 gaggctcccc tcgtgctcga cgaggagcgc gccgtccatc acgccgcgca cgacggcggg
     3301 gccgagtatg aggccgggcg cgggggcaag ctcctgcccg ccgttcggca ccgtcaggat
     3361 ttggtgctct tgggcttcca tgtttccgcc tccagcagat cgagatcggt gcccttcatc
     3421 ccctttgtcc gggcgccgtc caggttggtc gtttccttca tggtgaggtg catcgtggaa
     3481 ccggacaggt tcgtccggga gaagtcggcc agttcgaggt tggcatagct gaaatcggcg
     3541 tactcgaagt cgccacctgt ggccgtggcc tttcggaggt cggccttgat gaagcgggat
     3601 tcgcgcgctt tcgccccggc caggttggcg cgcgataact cagcttcctc gaacgaagcg
     3661 ccggcgatgt cggcgccggc gaacgaggca tcgttgaagg agcccttcct gcactcggcc
     3721 gaatccagct ttgcgcccgc gaaatcggcg ccatcgaacg tgccgccggt gagctccgcg
     3781 aacgtcatca cggtgttgcg gaagtttcca ccgcggcagt cggcgcccgc caggccgaac
     3841 tgggtgagct tgcaccctga gaagtcgatt ccggagaggt cggtcttgtt gcagctcgac
     3901 cggctgaagt cggtgg
//
LOCUS       DASVOD010000564         2442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_506844, whole genome shotgun sequence.
ACCESSION   DASVOD010000564 DASVOD010000000
VERSION     DASVOD010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506844"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF720_12465"
     CDS             complement(<1..921)
                     /locus_tag="VF720_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7880219.1"
                     /translation="MPPVLELKGIAKDYAGHRVVKGVDLVVQPGEIHALVGENGAGKS
                     TLMNILFGMPVIHSTGGFQGEVRLDGNPVRFSGPSDAMRAGIGMVHQEFMLIGGFSVA
                     ENIKLNREPLRANFLSGVIPSLATLDRKKIGADSRRVLDDVGLSLDEWTPVAGLPVGH
                     MQFVEIARELDKEKIRLLVFDEPTAVLTESEADRLLEVMKALSARGIAILFITHRLHE
                     VLAVAQGITVLRDGEIVARMNRETATIESIAAAMVGRQLDLSLADRRPAPGADAEAAL
                     DIRNLEVDMPGEEVRGVTLAVRKGEILGLGG"
     gene            complement(941..2107)
                     /locus_tag="VF720_12470"
     CDS             complement(941..2107)
                     /locus_tag="VF720_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HEX7880220.1"
                     /translation="MKRNVFGALALISLAALVGCGGGAKPADQAQNAPPAADKPAVAT
                     VDIPFKIGIMTGTVSQGEDEFRAAQMISEKYPGHVKHVTYPDNFMNEQETTMGQMTGL
                     AADPEVKVIIASQAVPGSVAALRKIREARPDIKIGFVEPHEDPAVVNAAADIIIQPDQ
                     LSRGRSIMEKAKKMGVKTFIHYSFPRHMSQELTALRRDEMKKAADELGIDFRFVTAPD
                     PMAEGGLPATQQFVLEDIPREVAQFGTETAFFSTNCGMMDPMIRQILATGAYFPEQCC
                     PSPTHGYPTALGISIPPDKAGDFAYISEQNRLKIAEAGKTGHFSTWAAPEVIVATRAM
                     VDLLVDSVQGKADYTDTATLQRYMSETAGVPVTAKKYDPNSGNSYLVLLDSIYY"
BASE COUNT          383 a          772 c          863 g          424 t
ORIGIN      
        1 ccccccgagg ccgaggattt cccctttccg cacggccagc gtgacgccgc gcacttcctc
       61 gcccggcatg tcgacctcga gattccggat gtcgagtgcg gcttcggcgt cggcgccggg
      121 ggcggggcgg cggtcggcca gggagaggtc gagctggcgc cccaccatgg cggcggcgat
      181 cgactcgatg gtggccgtct cccggttcat ccgggccacg atctcgccgt cacgcagcac
      241 ggtaatcccc tgggccacgg ccagcacctc gtgaagccgg tgcgtgatga agaggattgc
      301 gatgcctcgg gccgagaggg ccttcatcac ctcgagaagg cgatcggcct ccgactcggt
      361 aaggacggcg gtcggctcgt cgaacacgag gagccggatc ttttccttgt cgagctcgcg
      421 ggcgatttcc acgaactgca tgtggccgac cggcaggccg gcgacgggcg tccactcatc
      481 gagcgagagt cccacgtcat cgaggacgcg gcgcgaatcg gcgccgatct tcttccgatc
      541 gagcgtggcc agcgagggga tgacaccgga gagaaaattg gcgcggagcg gttcgcggtt
      601 gagcttgatg ttctcggcaa ccgagaagcc gccgatcagc atgaactcct ggtgcaccat
      661 gccgatgccg gcccgcatgg catccgatgg gccggaaaag cggaccgggt tgccgtcgag
      721 ccggacctct ccctggaacc cgccggtgga gtggatgacc ggcatcccga agaggatgtt
      781 catcagcgtg gacttgccgg cgccgttctc gccgaccagc gcgtggattt cccccggctg
      841 cacgacgaga tcgacgccct tcaccacgcg gtgaccggcg taatccttgg cgattccctt
      901 gagttcgagg acaggcggca cagtcgctcg gggcggccga tcagtagtag atgctgtcga
      961 gcaggaccag gtagctgttg ccgctgttgg ggtcgtactt cttggcggtg accggcacgc
     1021 cggccgtctc gctcatgtag cgctgcagcg tggccgtgtc ggtgtagtcg gcctttccct
     1081 gcaccgagtc gaccaggagg tcgaccatgg cgcgggtggc cacgatcacc tcgggggcgg
     1141 cccaggtgga gaagtgcccc gtcttgccgg cttcggcgat cttcagccgg ttctgctccg
     1201 agatgtaggc gaaatcgccc gccttgtccg gcgggatgga gatgccgagc gcggtggggt
     1261 agccgtgcgt ggggctcgga cagcactgtt cggggaagta ggcgcccgtg gccaggatct
     1321 gccggatcat cgggtccatc atgccgcagt tggtggagaa gaaggccgtc tcggtgccga
     1381 actgggccac ctcgcgcggg atgtcctcga ggacgaactg ttgcgtggcc gggaggccac
     1441 cctcggccat cgggtcggga gcggtgacga agcggaagtc gatgccgagt tcgtcggccg
     1501 ccttcttcat ctcgtcacgc cggagcgcgg tgagttcctg cgacatgtgg cgcgggaacg
     1561 agtagtggat gaaggtcttg acccccatct tcttggcctt ctccatgatc gagcggccgc
     1621 gcgagagctg gtcgggctgg atgatgatgt cggcggcggc gttcacgacc gccggatcct
     1681 catgcggctc gacgaatccg atcttgatgt cggggcgcgc ctcgcggatc ttgcggagcg
     1741 ccgccaccga gccgggaacc gcctgggagg cgatgatgac cttgacttcg gggtcggccg
     1801 cgaggcccgt catctgcccc atggtggttt cctgctcgtt catgaagttg tcgggatagg
     1861 tgacgtgctt cacgtgcccc ggatatttct ccgagatcat ctgagccgcg cggaattcat
     1921 cctctccctg gctgaccgtg ccggtcatga tgccgatctt gaacgggatg tcgacggtgg
     1981 ccacggccgg cttgtcggcg gcagggggcg cgttctgcgc ctggtccgcc ggcttggcac
     2041 caccgccgca gccgacgagc gccgcgaggg aaatgagggc gagcgcaccg aacacattcc
     2101 gtttcatgtc ttcctccggg atatcggatc gccggacggg agtccggcgc atggggctgt
     2161 cgaaccgacg cggggcggtc gaacgagatg ggccgaccgg tcgaaaccgc atggtagcag
     2221 aaaggttccg gcgtcggcac gggaccggaa agtggcccgt ggttgacgcc ggggcgcgcc
     2281 tcgcggcacc ttttcgcggc ccgaggaccc ccgacgcacc gccgcggaag tcggccagaa
     2341 ccggagaccc cgatgctcaa caccggcgac atcgcccccg atttcaccct gatcgggaac
     2401 gacgaccagg aatacaccct ttcggccctg cgcggccgga ag
//
LOCUS       DASVOD010000565         4497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_558289, whole genome shotgun sequence.
ACCESSION   DASVOD010000565 DASVOD010000000
VERSION     DASVOD010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4497
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558289"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VF720_12475"
     CDS             <1..843
                     /locus_tag="VF720_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HEX7880221.1"
                     /translation="AQSELAGRDSTFVDGLFSNGFKARKAERLGKGLADRIVAGGYPE
                     ALTRPTPRRRKAWYRDYVEALVQRDVRDLARIASLDALPRLLALAAGQTARLINVSDL
                     ASPFQISRPTIRDYVTLLERVFLLEELPPWHDNRLSRLVKTPKLHVGDTGVACALLGL
                     DAEALRADRNLLGQLLETFVFQELRRQASWHEAEIRFHHFREKDGAEVDIVLERGTKG
                     VVGVEVKASATVTPADFRGLRKLKEACGKRFVGGVVLYDGESSAGFGEGLQALPIRML
                     WESE"
     gene            complement(1231..2601)
                     /locus_tag="VF720_12480"
     CDS             complement(1231..2601)
                     /locus_tag="VF720_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX7880222.1"
                     /translation="MQMRIVPPVIGTRRRPLHQVLGPWSGAAVGIGIAIGAGIFRTPG
                     YVATFLPTGLAILGVWLLGGLLVIGDALILSELATRYPRAGGWYVYIEKAWGRFPAFV
                     YGWGFSLVVDPASSAALIVILGEYLASGLGLTPTSGRIAAIAVAWLFFVLSLIGVRVG
                     TRTQQALTWTKLLVLFGVAIMAFWLPAATGNTAPASPAPTAAGWGIVLAVGLALQGVL
                     WTFEGYANTTTMSEEAEDARKVLPRALIGGAIALTVAYLLMNAAYLHVLGRDSLAASM
                     LPGSDILGRLFGAAGQTIFLIVAIIAAAGSANGGVLTAPRVLYALARDGHAPEPFTRV
                     NKAGTPDLATLWFAVTWTLYAALGTFEGLVAVSIFIGAISNILVTLGLFRIRKRDGLV
                     TTDGRHPFLCPGWPVVPVVLLILWTALAVANLSSQGWGVGYGLLLVGLAAPVYVLVRK
                     RRDTVE"
     gene            2632..>4497
                     /locus_tag="VF720_12485"
     CDS             2632..>4497
                     /locus_tag="VF720_12485"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX7880223.1"
                     /translation="MTHDPNATDVPLEDANTIAPATPAPELVTVPPAAPAGAVMPWRR
                     PIPKWLIPFTIVSLLLIFIVWETRTSRTQAAILSKMASKLTYAMAPGPTDSVLYPVKG
                     PYDIRRGYTKIPGFTDTLRAQGYVVTGQARFSPKLLDYSKAGLFPIYHEKAQAGLNVY
                     DRGDRLIYGASYPERLYPDFESIPDLVVKSLLFIENRELLDPGRPYKNPAVEWDRLAK
                     AVIEYVGHKVHLKEDAPGGSTLATQIEKYRHSTEGRTSSANDKVVQMISATLRAYLDG
                     PDTERSRRGIVHEFVNSVPLAALPGYGEVNGLGDGLWAWYDADFARVNARLKELSIGF
                     AVDDKGALAFKQVLSLFIAHRRPSYYLIQDRERLAADTDSYIRLLAAGKLISPQLKEA
                     ALQAKLVFRKGTPDLENRTWVQRKAANAVRTRLLHLLDLKQLYDLDRVDLTATTTLDA
                     AAQDGVTHTLVRLTDPAFADSANLRGERLLAKGDPEGVIYSFTLYESSPQGNRLVVQA
                     DNYDQPLDINEGAKLDLGSTAKLRTLVNYLDIVCQIHAEHAGQSEATLRKVRVDRQDA
                     IKRWAIDWLASAPDTSLAAMLEGAMDRKYSSSTGEQFFTSGGLLTFGNFDKKDNGR"
BASE COUNT          846 a         1511 c         1453 g          687 t
ORIGIN      
        1 gcccagtccg agctggccgg ccgggactcc acgttcgttg acggactctt ctcgaacggc
       61 ttcaaggccc gcaaggccga gcggctcggc aaggggctcg ccgaccggat cgtggcgggc
      121 ggctatcccg aggcattgac acgcccgacg ccgcgacgcc ggaaggcgtg gtaccgggac
      181 tatgtcgagg cgctggtgca acgagacgtg cgggacctcg cccgcatcgc ctcgctcgat
      241 gcactccccc gcctgctggc gctcgccgcc ggccagacgg cccgcctgat caacgtcagc
      301 gacctcgcga gccccttcca gatcagccgc cccaccatcc gggactacgt gaccctgctg
      361 gagcgggtgt tcctgctgga ggaactgccg ccctggcacg acaaccggct cagccgcctg
      421 gtcaagaccc ccaagctcca cgtcggcgac accggcgttg cctgcgcgct gctcgggctc
      481 gacgccgaag cgttgcgcgc cgaccgaaac ctcctgggcc agttgctcga aacgttcgtg
      541 ttccaggagc tgcgccgcca ggcaagttgg cacgaagccg agattcgctt ccaccatttc
      601 cgcgagaagg atggcgccga agtcgacatc gtcctggagc ggggcaccaa gggagtcgtc
      661 ggtgtagaag tgaaggcatc ggccaccgtg accccggccg actttcgcgg gctgcgcaag
      721 ctcaaagaag cgtgcggcaa gcgtttcgtc ggcggggtcg tactctacga tggtgagagc
      781 agcgccggct tcggggaggg gctgcaggcg ctgccgatac ggatgttgtg ggaatccgag
      841 tgacaccact tgatggccag ttgctggaag cggacctgac gcacgacccg gccccactca
      901 cctggctcca gctcggaacg atctacatcg ggacgcttat caggatttga tggtcaatgt
      961 cagtgaccat cctcctggtc acttattaac acttaggcca tgaaatatta ataacggcct
     1021 cagttattca gatttgctta acaagggaag gaccacccca catcactctt ttccgtaacg
     1081 accgcacaca gatcgagttc accggccgga gacgaaaccg cctcttcgtc tacgacggtt
     1141 atctcaaggc gctgagcgag gggacggaac cgttgtgaat tgctggcagg ggggagttga
     1201 gacacaagga agccgtgagc aggggggcaa ctattcgact gtgtcccggc gcttcctcac
     1261 cagcacataa accggcgctg ccaaccccac cagcagcaac ccgtacccca ctccccaccc
     1321 ctgcgatgac aggttggcca cggccagtgc cgtccagaga atcaagagga cgaccgggac
     1381 gaccggccag cccgggcaga ggaaggggtg tcggccatcc gtcgtcacca gcccgtcccg
     1441 cttccggatc cggaacaatc ccagcgtcac caggatgttc gagatcgcgc cgatgaagat
     1501 gctcacggcg acgagtccct cgaacgtgcc gagagccgcg tagagcgtcc acgtcacggc
     1561 gaaccacagc gtggccaggt ccggcgtccc cgccttgttc acgcgcgtga acggttccgg
     1621 ggcatggccg tcacgggcca gggcgtacag gacgcggggc gcggtcagca cgccgccgtt
     1681 cgcgctgccg gccgcggcga tgatcgccac gatgaggaag atcgtctgcc cggcggcacc
     1741 gaacagtctc ccgaggatgt cgctccccgg aagcatcgag gcggcgaggc tatcccgtcc
     1801 gagcacgtgc aggtaggcgg cgttcatcag gagataggcg acggtgaggg cgatggcgcc
     1861 accgatgagc gccctgggca gcaccttgcg ggcgtcctcg gcctcctcgc tcatggtggt
     1921 ggtgttggcg tacccctcga atgtccagag gacgccctgc agggccaggc cgacggcgag
     1981 gacgattccc caccctgcgg ccgtgggcgc gggagatgcg ggtgccgtgt tgcccgtcgc
     2041 ggcgggaagc cagaaagcca tgatcgccac gccgaagagg accaggagct tggtccaggt
     2101 cagggcctgc tgcgtgcggg tgccgacgcg gacgccgatc agggaaagca cgaaaaagag
     2161 ccaggcaacg gcaatggcgg cgatgcggcc actggtggga gtcaggccaa ggccgctcgc
     2221 caggtattcg ccgaggatga cgatgagcgc agcgctcgaa gccggatcga ccaccaggct
     2281 gaatccccag ccgtagacga acgccgggaa tcgcccccac gccttttcga tgtacacgta
     2341 ccagccgccg gcccggggat accgggtggc gagctcggag aggatgaggg catccccgat
     2401 caccagtaat cccccgagga gccacacgcc aagaatcgcg aggccggtgg gaagaaacgt
     2461 ggcgacatag ccgggagtgc ggaagatgcc ggcgccgatg gcgatcccga tgccgacagc
     2521 ggcgccggac cacggaccga gtacctggtg aagaggacgc cgtcgtgtgc cgataactgg
     2581 cgggactata cgcatttgca tgaagggggc agtatactcc gcttacacga catgacccac
     2641 gatcccaacg cgaccgacgt gccgctggag gacgcgaaca ccatcgcgcc cgccaccccc
     2701 gcgccggagc tcgtcacggt gccccccgcc gcgccggccg gtgcggtcat gccatggcgt
     2761 cgcccgattc cgaaatggct gattcccttc acgatcgtgt cgctcctcct catcttcatc
     2821 gtgtgggaga cgcggacgtc gcggacgcag gccgccatcc tctcgaagat ggcgtcgaag
     2881 ctcacctatg ccatggcgcc cgggcccacc gactcggtgc tctacccggt caaggggccg
     2941 tatgacatcc ggcgcgggta cacgaagatt ccgggattca ccgacacgct gcgcgcgcag
     3001 gggtacgtgg tgaccggaca ggcccgcttc tcgccgaagc ttctcgatta ttcgaaggcc
     3061 ggcctctttc ccatctacca cgagaaggca caggccgggc tcaacgtgta cgaccggggc
     3121 gatcggttga tctacggcgc ctcttatccc gaacggctct acccggattt cgaatcgatc
     3181 cccgacctgg tcgtgaagag cctcctcttc atcgagaacc gcgaactgct cgacccgggg
     3241 cgtccgtaca agaatccggc cgtcgagtgg gatcgcctgg ccaaggcggt catcgagtac
     3301 gtcggtcaca aggtgcatct caaggaagat gccccgggtg gcagcacgct ggccacgcag
     3361 atcgagaagt accgccactc caccgagggg cgcacctcga gcgccaacga caaggtggtg
     3421 cagatgatca gcgccacgct ccgcgcttac ctggacgggc ccgacaccga gcgttcgcgc
     3481 cgcgggatcg tgcacgagtt cgtgaactcg gtgcccctgg cggcacttcc cggttacggc
     3541 gaggtcaacg ggctcggcga cggcctctgg gcctggtacg acgccgattt cgcccgggtg
     3601 aacgcgcgtc tcaaggagtt gtcgatcgga tttgcggtgg acgacaaggg agcgctggcc
     3661 ttcaagcagg tgctctcgct cttcatcgcc catcgtcggc cgagctacta cctcatccag
     3721 gatcgtgagc gcctggccgc cgacaccgac tcgtacatcc gcctcctggc cgccggcaag
     3781 ctgatctcgc cgcagctgaa ggaggcggca ctccaggcca aacttgtttt ccgcaaggga
     3841 acgcccgacc tcgagaaccg gacgtgggtg cagcggaagg ccgccaatgc cgtgcgcacg
     3901 cgcctgctcc acctgctcga cctgaagcag ctctacgacc tggaccgcgt ggacctgacc
     3961 gccaccacca cgctcgacgc cgccgcgcag gacggcgtga cccacacgct ggtgcgcctc
     4021 accgatcccg ccttcgccga ctcggccaac ctgcgcggcg agcgactgct ggccaagggc
     4081 gatcccgaag gggtcatcta cagcttcacg ctgtacgaga gctcaccgca ggggaaccgc
     4141 ctggtcgtgc aggccgacaa ctacgaccag ccgctcgaca tcaacgaggg agccaagctc
     4201 gacctgggct cgacggccaa gctgcgcacg ctggtcaact atctcgacat cgtctgccag
     4261 atccacgccg agcatgccgg gcagtcggaa gccacgctcc gcaaggtgcg cgtggaccgg
     4321 caggacgcca tcaagcgctg ggccatcgac tggctcgcgt cggccccgga tacctcgctg
     4381 gcggccatgc tcgagggcgc gatggaccgg aagtattcgt catcgaccgg cgagcagttc
     4441 tttacgtccg gcggactgct gacgttcggc aatttcgaca agaaggacaa cggccgg
//
LOCUS       DASVOD010000566         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1031884, whole genome shotgun sequence.
ACCESSION   DASVOD010000566 DASVOD010000000
VERSION     DASVOD010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031884"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..907
                     /locus_tag="VF720_12490"
     CDS             32..907
                     /locus_tag="VF720_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005111464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX7880224.1"
                     /translation="MADEELVFDDAGLNKWTDDDHFEVTREHLIDYAKATNDPIPAHL
                     AGDVAPPIFAIVPVFESLLTPTVDVAPVELIPRVVHGEQDFHFHRPIYPGDKLVSRGM
                     MLGYEGLENGTRASVYLECRTDDGDLVNEQYVTCFFRGFNAGKKIGELGPSHKFDTAL
                     RDEPPLAKVIQHVDADQTFRYSPASGDPMPIHLDEEVAKDAGLPGIIAHGLCTMAFTS
                     WALLTEVGGEDVNRLKRFAVRFSKMVFPGDDLETRIWQAGRGGHTTRYAFETVRNGSA
                     GEEFAITDGLAVFAD"
     gene            930..1796
                     /locus_tag="VF720_12495"
     CDS             930..1796
                     /locus_tag="VF720_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7880225.1"
                     /translation="MGALAGRIAVITGAGRGIGREHALLFAREGASVVVNDLGGSNTG
                     EGADAGPAQEVVDEIVAAGGKAVANTDNIATWAGAAALIEQAVSEFGGLDVVVNNAGI
                     LRDGFIPTMDESQWDAVVSVHLKGHFSVLRHAAAYWKAQSKAGNQPNAAVINTASGSG
                     LTVPNAGQVNYGAAKAGIAAMTLVAADELERYGVRANAIAPIARTRLTLATPGMGALM
                     AEPDDGEVDLFSPANISPLVAYLATEKCPLTGKVFAVQGGAISELGGWHDLTTIETDG
                     LWEIDDIAGRLS"
BASE COUNT          351 a          581 c          621 g          306 t
ORIGIN      
        1 ctcgacgcgc tggccgcggc ggcggtctga aatggccgac gaggaactgg ttttcgatga
       61 tgctgggctc aacaagtgga ccgacgacga ccacttcgag gtcacccggg agcacttgat
      121 cgactatgcg aaggccacca acgatcccat cccggcgcac cttgccggtg atgtggcccc
      181 gccgatcttc gcgatcgtgc cggtgttcga atccttgctg actccgaccg tcgacgtggc
      241 gccagtggag ctgattcccc gggtggtgca cggggagcag gactttcact tccaccggcc
      301 gatctacccg ggtgacaagc tggtgtcgcg cggcatgatg ctcggctacg aagggctcga
      361 gaacggcaca cgcgcatcgg tctacctgga atgccggacc gatgatggtg acctcgtcaa
      421 cgaacagtac gtgacgtgct tcttccgcgg gttcaatgcc ggcaagaaga tcggcgaatt
      481 gggcccgagc cacaagttcg acaccgctct gcgcgacgaa cccccgctgg ccaaggtcat
      541 ccagcatgtc gatgccgacc agacgttccg ttactcaccg gcatcggggg atccgatgcc
      601 gatccacctc gacgaggagg tggccaagga tgccgggctg ccgggcatca tcgcccacgg
      661 cctgtgcacc atggccttca cgtcgtgggc cttgctgacc gaggtcggcg gcgaagacgt
      721 caaccggctc aagagatttg ccgtccggtt ctccaagatg gtttttcccg gtgatgacct
      781 ggagacccga atctggcagg ccggccgcgg cggccacacg acgagatacg cgttcgagac
      841 cgtccgaaac ggcagtgccg gcgaggaatt cgccatcact gacggcctgg ccgtattcgc
      901 cgattagttg agttgagagg acttcagaca tgggagcttt ggcaggacgg atcgccgtca
      961 tcaccggcgc ggggcgcggc atcggccgcg agcacgcatt gctgttcgcc cgggaaggcg
     1021 ccagcgtggt cgtcaacgac ctcggtggca gcaacaccgg cgagggggca gacgcgggac
     1081 cggcgcaaga ggtcgtcgac gagattgttg ccgccggcgg caaagcagtc gccaataccg
     1141 acaacatcgc cacctgggct ggggcagcgg ccctgatcga acaggcagtc agtgagttcg
     1201 gcggtctgga cgtggtggtg aacaatgccg ggatcttgcg tgacggcttc atcccgacga
     1261 tggatgaatc ccaatgggac gccgtggttt cagtacatct caaaggccac ttctcggtgc
     1321 tccggcacgc ggcggcgtac tggaaggcgc aaagcaaggc cggcaaccag ccgaacgccg
     1381 cggtgatcaa cacggcatcg ggatcgggcc tcaccgtccc gaacgccggg caggtgaact
     1441 acggtgcggc caaggccggc atcgcggcga tgaccttggt cgcggccgat gaactggaac
     1501 gctatggcgt gcgggccaac gctattgcac ccatcgcccg gacccggctc accttggcca
     1561 cccccggcat gggcgcactg atggctgagc ccgacgacgg cgaggtcgac ctgttcagcc
     1621 cggcgaacat ctcgccgttg gtggcctacc tggcaacgga gaagtgcccg ctgaccggca
     1681 aggtcttcgc ggtgcagggc ggcgcgatct ccgagctcgg cggctggcac gacctgacaa
     1741 ccatcgagac cgacggtctg tgggagatcg acgacatcgc ggggaggctg tcgtgatcga
     1801 gtggtccgaa tccgacgagc tcattcgcga gagcgtccgc gagttcatcg acaaataca
//
LOCUS       DASVOD010000567         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_819679, whole genome shotgun sequence.
ACCESSION   DASVOD010000567 DASVOD010000000
VERSION     DASVOD010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819679"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VF720_12500"
     CDS             complement(<1..565)
                     /locus_tag="VF720_12500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7880226.1"
                     /translation="MSPTHTSLPKSVDIVIIGGGIAGLAAATELAARKPGRILLIEQE
                     RAPGLHSTARNASLLLSDTEDPVAARVAMASRPFYADTAEDEPPRFRPAGSLLLFSGP
                     EAETRARARIDGVAPLGLAASYLTPDDIRSRVPLLRTGHLGAGVFAAADGVIEIGPTV
                     EALTDRAVRGGVLLMCGVRAIKLVTDEG"
     gene            complement(571..>1617)
                     /locus_tag="VF720_12505"
     CDS             complement(571..>1617)
                     /locus_tag="VF720_12505"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX7880227.1"
                     /translation="LKETLLYLTDSGAEHLREYGNLSKATVGVLLRKIVEMEQQGGEQ
                     FFGEPEFNVDDLLTVDGDGRGRVSILNLADIQDKPALFSTFMMWLLATLFRELPEAGD
                     LAKPRLVFFFDEAHHLFAGKGKAFEEQIEQVVRLVRSKGIGVWFVTQSPRDLPADVLA
                     QLGNRVQHALRAFTPDDDKAIRATARTMPKSGFYDVADQLTRLGIGEAFVTVLGPNGV
                     PTPVAATLLMPPASSMTPLPPVEVSRRAAASPLHERYGTEIDRQSAREALQARVAEET
                     ALAEAANRPDPAASRTPTGRTTASPRPAARARSSLDAAQTILKSPVARAATQELVRGI
                     FGLLGVKTSRRRAW"
BASE COUNT          246 a          548 c          580 g          243 t
ORIGIN      
        1 gaccctcgtc cgtcaccagc ttgatcgccc gcacgccgca catgaggagg acaccgcccc
       61 gcaccgcgcg gtccgtgagt gcctccactg tggggccgat ctcgatgacg ccgtcggccg
      121 ccgcgaacac gccggcgccg aggtgaccgg tccggagcaa cggaacgcgc gagcggatgt
      181 cgtccggcgt gaggtacgaa gccgccagac ccagcggcgc cacgccatcg atccgggcac
      241 gggctcgcgt ctccgcttcc ggcccggaga aaaggaggag cgaacctgcc gggcggaagc
      301 gcggcggctc gtcctcggca gtgtccgcgt agaacggacg actggccatc gccacgcggg
      361 cggccaccgg gtcctccgtg tccgaaagaa ggagggacgc gttgcgcgcg gtggagtgca
      421 gtcccggcgc gcgctcctgt tcgatgagga ggatgcgtcc gggttttcgt gccgccagct
      481 cggtggccgc ggccaggccg gcgatgcccc cgccgatgat gacgatgtcg accgacttgg
      541 gaagcgaggt gtgcgtcggg ctcatgacgg ttaccaggcg cgccggcgcg aggtcttcac
      601 gccgagcagt ccgaaaatgc cgcgcacgag ttcctgcgtg gccgcccggg ccaccgggct
      661 cttgaggatc gtctgggcgg catcaaggga gctgcgcgcg cgcgcggcgg gccgcgggga
      721 cgcggtcgtg cgacccgtcg gcgtccgcga cgccgccgga tcagggcggt tcgccgcctc
      781 cgccagggcg gtttcctccg ccacccgtgc ctggagagcc tcacgggcgc tctgccggtc
      841 gatctcggtt ccgtagcgct cgtgcagggg agaggccgcg gctcgtcgcg acacctcgac
      901 cggcgggagc ggcgtcatgc tcgaggccgg cggcatgagg agggtggccg ctaccggagt
      961 cgggacgccg ttcggcccga gcaccgtcac gaaggcctcg ccgatgccga gcctggtcag
     1021 ctggtcggct acgtcgtaga acccggattt cggcatggtg cgggcggtag cccgaatggc
     1081 cttgtcgtcg tcgggcgtga aggcgcgcag cgcgtgctgc acgcggttgc cgagctgggc
     1141 cagcacgtcg gccggcagat ccctgggcga ttgcgtcacg aaccagacgc ctattccctt
     1201 cgagcgcacg aggcgcacga cctgctcgat ctgctcctcg aacgctttcc ccttccccgc
     1261 aaagaggtgg tgagcctcgt cgaagaaaaa gacgagccgc ggcttcgcca ggtctcccgc
     1321 ctcggggagc tcgcggaaga gggtggccag cagccacatc atgaaggtgc tgaagagggc
     1381 gggtttatcc tggatgtccg cgaggttcag gatcgacacg cgcccccggc catcgccgtc
     1441 gacagtcagg agatcgtcca cgttgaactc cggctcaccg aaaaactgct cgcctccctg
     1501 ctgctccatc tcgacgatct tgcgcagcag cacgccgacc gtggccttcg agaggttgcc
     1561 atactcgcgc aggtgctcgg cgccggaatc ggtcaggtaa agaagcgttt ccttgag
//
LOCUS       DASVOD010000568         2809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_871631, whole genome shotgun sequence.
ACCESSION   DASVOD010000568 DASVOD010000000
VERSION     DASVOD010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871631"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..2307
                     /locus_tag="VF720_12510"
     CDS             109..2307
                     /locus_tag="VF720_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880228.1"
                     /translation="MPADSLRRVTPFALIFVAALPMTLAAAIPATPAALHSKPPPDHP
                     AGSPSSPPALIDAQHWPIEAGDTWRYLVRRVERRPGDDLVLEDSAEVRVLASFRHGRR
                     RVAALEWRRPDFDLLPPWVKSDLPLIVPRRIDVEYQVLDDSRLVMVDDPERLGVVMAS
                     TGNGAPEEETRVPLDDLPLPLVEGGRWGAPAMLARPDGLYTGTTGALTTLSLPIGQVR
                     AVPVTYRTLPDREDRWLSPDLGLVRRQYRHQGLDLLETWELAAFTHEAVDSLALANRI
                     DARVRELIKGEGSWSRRSALASDPVLAALLGPMVATETPEDDDGVRRIFLRGASGQLT
                     LERLPDGPTRAWRVARASGGVVRRRFTISDDVPRPLPPGTSRLDLAEVNSLFETARRS
                     ALQGDARGAAGRVEAAMQILTGRLIAAAPESGTSRAPAEAWRETLGAAAGDSVLVTRY
                     GSRVRIDDDDLRVDLEIDRDLWALTARRAGPDGGWIGGAPRLVLGTAPPVVLVEPEDS
                     ITDLAPRRWLTLDWGLDHPGLDVLVLAESPTGLARRLDLFRVDKGRAERGWTRLLAGG
                     RARMAGDSRRLDVESERPDPRLSAAVQHWRESYGPPDSPESISPVPLRWERLDPWLDV
                     AVSAVEWLRRSDTATAPPSIRGEGLAVRLASTWLARRDVRVARVLSYRAAPNDELSSP
                     VASVAFHRERAAGRAEAEPEVLSLRLVREHGEWVVVDLVAGDVDSGEWER"
     gene            2304..>2809
                     /locus_tag="VF720_12515"
     CDS             2304..>2809
                     /locus_tag="VF720_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880229.1"
                     /translation="MNRHRIAFIVIFGTAMGFLEAAVVVYLRLLYYPSGFDFPLVLLP
                     SITVGVELGREAATIVMLWGVAELTGRNGVERFGWFSILFGVWDIIYYVALKAVLDWP
                     ASLLTWDVLFLIPLVWTGPVLAPVIVSIGLIAGGARLVAFGERGIPIRLTLLDRATLG
                     LSLLLLLVA"
BASE COUNT          346 a          988 c          991 g          484 t
ORIGIN      
        1 gccgccgggc gctggcgctc tggcgcacca tcggggccct ggacgaagag caggggcgaa
       61 cggcggcccg gcaggcggtg gatgaacggg tggcgcgcct cagggcctgt gccggctgat
      121 tccctccggc gcgtcacgcc cttcgcgctg attttcgtcg cggcacttcc gatgacgctg
      181 gccgccgcaa ttccagcaac cccggccgcc ctccactcga agcctccccc cgaccatccg
      241 gcggggtctc cgtcatcacc tcccgcgttg atcgacgcgc agcactggcc gatcgaggcg
      301 ggggacacct ggcgatatct cgttcgacgc gtggaacgcc ggccgggcga cgacctggtc
      361 ctggaagact cggccgaggt gcgtgtgctc gcctcctttc ggcatggccg gcggcgcgtg
      421 gccgcgctcg agtggcgtcg ccccgatttc gacctcctgc cgccatgggt caagagcgat
      481 ctcccgctca tcgtgccccg ccggatcgat gtggagtacc aggtcctgga cgactcccgg
      541 ctggtgatgg tcgatgatcc ggaacgcctc ggcgtggtca tggcgtccac cggaaacggc
      601 gctccggaag aggaaacccg ggtgcctctc gatgacctgc cgcttccgct ggtggaggga
      661 gggcgctggg gcgcgccggc gatgctggcc cgacccgacg gcctctatac ggggacgacg
      721 ggggcgctca ccacgctctc cctgccgatc ggccaggtgc gggccgtgcc cgtcacctac
      781 cgcaccctcc cggatcgcga ggatcgctgg ctgtccccgg atctgggact cgtccgccgc
      841 cagtaccgtc atcagggact cgatctgctg gagacgtggg aactggccgc attcacgcac
      901 gaagcggtcg acagcctggc cctggccaac cgcatcgatg cccgggtgcg ggagctcatc
      961 aagggagaag gatcgtggtc gcgtcggtcg gccctcgctt ccgacccggt gctggccgcg
     1021 ctgcttgggc ccatggtcgc gacggagacg ccggaggacg acgacggcgt gcgtcgcatc
     1081 ttcctgaggg gcgcgtcggg tcaactcacc ctggaacgac ttcccgacgg accgacgcgc
     1141 gcgtggcgcg tggcccgggc ttcaggcggc gtggttcgcc ggcgattcac catctccgac
     1201 gacgtgccgc gcccgctgcc tcccggcacg tcgcgcctcg acctcgccga agtcaactct
     1261 cttttcgaga cggcccgtcg ttccgccctc cagggcgatg cccgcggggc cgccggacgc
     1321 gttgaggcag ccatgcagat ccttaccggg cgcctcatcg ccgccgcccc ggagtcgggc
     1381 acgtcccgcg cgccggccga agcgtggcgg gaaacgctcg gtgccgcggc gggcgattcc
     1441 gtcctcgtca cgcggtacgg ctcgcgtgtc cgtatcgacg acgacgacct gcgcgtcgat
     1501 ctggaaatcg atcgggacct ctgggcgctc accgcccggc gggcgggacc cgacggcggc
     1561 tggatcggcg gagcgccccg actcgtgctc ggcacggcgc cgccggtcgt actcgtcgag
     1621 ccggaggatt ccatcaccga cctggcgccg cgtcgctggc tgacattgga ctgggggctc
     1681 gaccatccgg gcctcgatgt cctggtgctg gccgaaagcc ccaccggcct ggcccgccgg
     1741 ctcgacctct tccgtgtgga caaggggcgc gccgagcgag gctggacgcg cctcctggcg
     1801 ggcggacggg cacgcatggc gggtgattcg cgccggctgg atgtcgagtc ggaacgtccc
     1861 gacccgcggt tgtcggcggc ggtgcagcac tggcgcgagt cctacggccc gccggattcc
     1921 ccggaatcga tctcacccgt cccgctgcgc tgggagcgcc tcgacccctg gttggacgtg
     1981 gccgtctcgg ccgtggaatg gttgcgaagg tcggacacgg ccaccgcacc tccttccatt
     2041 cgtggcgaag gtctcgcggt ccgcctggct tccacctggc tggcgcgtcg ggacgtccgc
     2101 gtcgcgcggg tgctgtccta ccgcgcggca cccaacgatg agctctcgtc gccggtcgcc
     2161 tcggtggcat ttcatcggga acgggccgcc ggccgcgccg aggccgagcc ggaagtgctc
     2221 tcgttgcgcc tggttcgtga gcatggcgag tgggtggtcg tggacctcgt ggccggggat
     2281 gtcgattcgg gggaatggga acgatgaacc ggcaccgcat cgcattcatc gtgatcttcg
     2341 gcaccgcgat gggattcctc gaagccgccg tggtcgtgta cctccggctc ctctattacc
     2401 cctccggatt cgacttcccc ctggtgctcc ttccttccat caccgtcggc gtggagctgg
     2461 ggcgcgaggc agccacgatc gtcatgctct ggggcgtggc ggagctgacc gggcggaacg
     2521 gggtggaacg gttcggctgg ttctccatcc tgttcggcgt gtgggacatc atctattacg
     2581 tggcgctcaa ggccgttctc gactggccgg cctcgctcct cacgtgggat gtgctgttcc
     2641 tcattcccct ggtgtggacc ggcccggtgc tggctccggt gatcgtgtcg atcggcctga
     2701 tcgcgggagg ggcgcgcctt gtggccttcg gggagcgggg gattccgatc cgactgacgc
     2761 ttctcgaccg ggccactctc ggcctgtcgc tgctcctcct gctcgtggc
//
LOCUS       DASVOD010000569         3630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_401227, whole genome shotgun sequence.
ACCESSION   DASVOD010000569 DASVOD010000000
VERSION     DASVOD010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401227"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VF720_12520"
     CDS             complement(<1..344)
                     /locus_tag="VF720_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEX7880230.1"
                     /translation="MIDGVILKPLKVIPDERGFLMEILRKDDPFFKEFGQVYLTVVYP
                     DVVKGWHYHRKQTDHFCVVKGMSKVVLYDSREGSRTFGEVNEFFLGDHNRTLLVIPPL
                     VLHGMKGVGTEPA"
     gene            complement(355..1695)
                     /locus_tag="VF720_12525"
     CDS             complement(355..1695)
                     /locus_tag="VF720_12525"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007680599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX7880231.1"
                     /translation="MYKICMVGTGYVGLVSGACFADFGNTVICVDKDASKVERLKKGE
                     IPFYEIGLDEIVARNLKEGRLSFSTDLASAVKKCEVIFIAVGTPYGRGGEADLSYVWG
                     AAKEVAQAMNGYRLIVQKSTVPVGTGAKIRELVAKSLKKKTPFDVASNPEFLREGSAI
                     GDFMRPDRVVIGTSSQKAEEILAGIYAPLYLNETPMVRTNVETAELIKYAANAFLATK
                     ISFINEMANLCEIVGADVKIVAKGMGLDNRIGGKFLHAGAGYGGSCFPKDTSALATFS
                     RKAGYPLKIVKATIDVNDGQRERILGKITKTIGAAKGKTFGVLGLSFKPNTDDVRDSV
                     ALDLIKALLKAGARVKAFDPVAMDNAKKALPKQVHFAEDVWETVDGADCLILATEWNE
                     FRRLDMARAKRVMKRPVVIDCKNVYDPKAMADMGFTHVGVGRGIPLTQQKRTDG"
     gene            complement(1836..2921)
                     /locus_tag="VF720_12530"
     CDS             complement(1836..2921)
                     /locus_tag="VF720_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX7880232.1"
                     /translation="MDPRSRPHEWTPLSTEPLNGGTHPTGLADLLYTPASRFFVRNHG
                     PVPEIDLASWRLRVDGLVAHALEFTFDELIGRYRRTRRPATIQCAGFRRTGLSRVRPI
                     PGELPWSEESVGTAEWEGVLLADVLADAAPRPGARHAAFEGCDVCEREERRFGFGGSV
                     WIEEVAAQGILLATSMNGEPLSAEHGWPLRVVAPNVIGARSVKWLTRIELRQDPSDNY
                     FQARTYKILPMPQPTRNEWSAAPPLGRLPVNALICRPGEGASVPAGRVAVGGFAWTGH
                     EEGISRVELSTDGGHAWQPARLEPAEMRGVWQRWTAEPTLAPGRHELVVRAVDAGGAC
                     QPDDPALLWNARGYLNNAWQRVAIDVA"
     gene            complement(2971..>3630)
                     /locus_tag="VF720_12535"
     CDS             complement(2971..>3630)
                     /locus_tag="VF720_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880233.1"
                     /translation="RARPLRPFVSRPPAWLRGTLSALPDFALALFILSVWQDPLAHGD
                     RMVGNLLLLMLLEFIIVHASGFMASIIHGPSDRLRKVGQLFLLGLLESVFVGGFSLSF
                     GAWWPLASFWGLTVNRMLGVLLNPPSDQDNRIILGSQWAMSVVFYLGGVFLTLLGPVP
                     RLGLTPDVMPRLGLTGGGIWIDQPWRVLAFGVTYFATQGAWALIVPFLFHGPESASRR
                     A"
BASE COUNT          613 a         1204 c         1209 g          604 t
ORIGIN      
        1 gccggctcgg tgcccacgcc cttcatcccg tggaggacca ggggggggat gacgaggagc
       61 gtccggttgt ggtcccccag gaagaactcg ttgacctccc cgaacgtgcg gctcccttcg
      121 cgcgagtcgt acaggacgac cttgctcatt cccttgacga cgcagaaatg atccgtctgc
      181 ttccggtggt aatgccaccc cttcaccaca tccgggtaga ccaccgtgag gtacacctgt
      241 ccgaattcct tgaagaaggg atcgtccttc cggaggatct ccatgaggaa gccccgctcg
      301 tcggggatga ccttgagggg cttcaggatg acgccgtcga tcacgattcg gccctcagcc
      361 gtcggtgcgc ttctgctggg tcagggggat gccccggccg acgcccacgt gcgtgaatcc
      421 catgtcggcc atggccttgg gatcgtaaac gttcttgcag tcgatgacca cgggacgctt
      481 catgacgcgc ttggcgcgcg ccatgtcgag ccggcggaac tcgttccact cggtggccag
      541 gatgaggcag tcggcgccgt cgacggtttc ccacacgtct tcggcgaagt gcacctgctt
      601 cggcagcgcc tttttcgcgt tgtccatggc caccgggtcg aacgccttca cgcgggcgcc
      661 cgccttgagc agcgccttga tgaggtcaag cgccacggag tcgcggacgt cgtccgtgtt
      721 gggcttgaac gaaagcccca gcacgccgaa cgtcttccct ttcgcggcgc cgatcgtctt
      781 cgtgatcttg ccgaggatcc gctcgcgctg gccgtcgttg acgtcgatgg tggccttcac
      841 gatcttcagc ggataaccgg ccttccggct gaacgtggcc agggccgagg tgtccttggg
      901 aaagcacgag ccgccgtacc cggccccggc gtgcaggaac ttgccgccga tccggttgtc
      961 gaggcccatc cccttggcca cgatcttgac gtcggcgccg acgatctcgc acaggttggc
     1021 catctcgttg atgaaggaga tcttggtggc caggaaggcg ttggcggcgt acttgatcag
     1081 ctcggccgtc tccacgttcg tgcgcaccat cggcgtttcg ttcaggtaga gcggggcgta
     1141 gatgccggcc agaatctcct cggccttctg gctggaggtg ccgatgacca cgcggtcggg
     1201 gcgcatgaag tcgccgatgg cgcttccctc cctgaggaac tcggggttgc tggccacgtc
     1261 gaagggcgtc ttcttcttga gcgatttcgc caccagctca cggatcttcg caccggtgcc
     1321 gacgggtacg gtgctcttct ggacgatcag ccggtagccg ttcatggcct gggccacttc
     1381 cttcgcggcg ccccacacgt aggagaggtc ggcttcaccc ccccggccgt acggcgtccc
     1441 cacggcgatg aagatgacct cgcacttttt taccgccgag gccaggtccg tcgagaagga
     1501 gagcctccct tccttcaggt tacgggccac gatttcatcg aggccgatct cgtagaaggg
     1561 gatctccccc ttcttcagcc gctccacctt cgacgcgtcc ttgtcgacgc agatgaccgt
     1621 gttgccgaag tcggcgaagc aggcgccgga aacgagtccg acgtagccgg tgccgaccat
     1681 gcagatcttg tacatgagct ctcccgaagg ggtgcggtgg gccgggggcg gaaaatgtca
     1741 ggcccgtaag tgaaaaagac gcaagggagt atacgcgacg gttcaaggcg tctcaagcaa
     1801 aaaccggctg ccaggaggtc tccagccggg cctgttcaag ccacgtcgat ggccacccgt
     1861 tgccaggcat tgttgaggta acctcgggcg ttccagagga gcgccggatc gtccggctgg
     1921 caggctcccc cggcgtcgac ggcgcgcacc accagctcgt ggcgccccgg ggcgagcgtc
     1981 ggttcggccg tccagcgctg ccagacgccc ctcatctccg ccggctcgag ccgggccggc
     2041 tgccaggcgt gtccaccgtc ggtcgagagc tccacgcggg agatcccctc ttcgtgcccc
     2101 gtccacgcga agcctccgac tgccacccgg cccgccggca ccgaggcccc ctcgcccggc
     2161 cgacagatga gcgcgttcac cggcagacgg ccgagaggcg gcgcggcgct ccactcgttc
     2221 cgcgtgggtt gcggcatcgg caggatcttg taggtgcgcg cctggaaata gttgtcggac
     2281 ggatcctggc ggagttcgat ccgggtgagc cacttcacgc tgcgcgcccc gatcacgttc
     2341 ggcgccacca cgcgcaacgg ccagccatgc tcggcgctga gcggctcgcc attcatcgac
     2401 gtggccagaa ggattccctg cgccgccacc tcctcgatcc acaccgaccc gccgaagccg
     2461 aaccgacgct cttcgcgctc gcacacgtcg cacccttcga aggcggcgtg ccgcgcgccg
     2521 ggtcgtggag cggcatccgc caggacatcg gccaggagca ctccttccca ttccgcggtg
     2581 cccaccgact cctcgctcca ggggagttcg ccggggatcg gcctgacgcg tgacagcccc
     2641 gtgcgacgaa atccggcgca ctggatcgtg gccggtcgac gggtgcgtcg gtagcggccg
     2701 atcagctcgt cgaaggtgaa ttcgagggcg tgcgccacca ggccgtcgac gcgaagccgc
     2761 cacgaggcga ggtcgatctc aggcacgggg ccatggttgc ggacgaagaa acgggaagcg
     2821 ggcgtgtaga gaaggtcggc gagcccggtg ggatgagttc cgccgttcag gggttcggtg
     2881 ctgagcggcg tccactcgtg cgggcgcgac ctcgggtcca tgacacgatc atatcatggc
     2941 atcccgatag acttggtagg gatcccggag tcaggcccgc cggctcgccg actccggacc
     3001 atggaagagg aacggaacga tcagggccca cgcaccctgc gtggcgaagt aggtgacgcc
     3061 gaacgccagg acacgccagg gctggtcgat ccagatgccg cccccggtga ggcccaaccg
     3121 cggcatcacg tcgggcgtca ggccgagtcg cggcaccggc ccgaggagcg tgaggaacac
     3181 cccacccagg tagaagacca cgctcatcgc ccattggctg ccgaggatga tgcggttgtc
     3241 ctggtccgat ggcgggttca gcagcacgcc gagcatccgg ttcaccgtga gtccccagaa
     3301 gctggccagc ggccaccacg ccccgaagct gagcgagaag ccgccgacga agaccgattc
     3361 gaggaggccg aggaggaaga gttgccccac cttgcgcagg cgatccgacg gaccgtggat
     3421 gatcgaggcc atgaatcccg aggcatgaac gatgatgaac tcgagcagca tgaggagaag
     3481 gagatttccc accatccgat ccccgtgcgc gagggggtcc tgccagaccg acaggatgaa
     3541 gagggcgagc gcgaaatcgg ggagtgccga gagcgtgccg cgcagccagg ccggcggccg
     3601 gctcacgaac ggtcggagag gccgcgctcg
//
LOCUS       DASVOD010000570         8526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_662390, whole genome shotgun sequence.
ACCESSION   DASVOD010000570 DASVOD010000000
VERSION     DASVOD010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662390"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..734
                     /locus_tag="VF720_12540"
                     /pseudo
     CDS             384..734
                     /locus_tag="VF720_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
     gene            863..1378
                     /locus_tag="VF720_12545"
     CDS             863..1378
                     /locus_tag="VF720_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX7880234.1"
                     /translation="MSRPTTPGYAHGTGYGQKTAMDLQSVFGDAVSNWREFRGDWHIT
                     VAPARLRDVAYHLRDTLGYQMLSSMSGVDYYPDEPRFKVVYMLTHMVEKTQFLFEAFT
                     GGENPELPTLTDIWMVADWQEREVYDFFGIKFTGHPDLRRVLMPEDFEGFPLRKDFPL
                     LGVDVGLKARS"
     gene            1398..1922
                     /gene="nuoE"
                     /locus_tag="VF720_12550"
     CDS             1398..1922
                     /gene="nuoE"
                     /locus_tag="VF720_12550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015259866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX7880235.1"
                     /translation="MLSEKTKKDILAIKDRYPDRRSATLPALYAAQEDQRWLSSETIQ
                     EVADLLEMPVRMLHEVATFYVMFETKPVGTHLVEVCDNISCALRGGEEIIQYLERKWG
                     IKRGGTTPDGKFSLRGAECLGACGTAPCIMIDHRYWENLTFAELDKRLAALTDDPHHT
                     RGDLAEPPIPAGKS"
     gene            1922..3250
                     /gene="nuoF"
                     /locus_tag="VF720_12555"
     CDS             1922..3250
                     /gene="nuoF"
                     /locus_tag="VF720_12555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEX7880236.1"
                     /translation="MAPSFPLILTKNMHRADSHTLAAYEASGGYKAWKKTLDGLTPDQ
                     VIATVKDSGLRGRGGAGFPCGMKWSFVPRQSEKPKYLACNADESEPGTFKDRMLMTRD
                     PHMLIEGMAIASFAIDCHLAFIYIRGEFAIEAVILEAAIEEARQAGHLGRNIYGKGYD
                     LDILVYRGAGAYIAGEETGMLESLEGKRAHPRIKPPFPAVVGLYGAPTVINNVETLAN
                     VPAIIENGADWFKAIGTPKSTGTKLMNISGHVNRPGVVELPMGTPLMTIINDYCGGVW
                     HNRKLKAVIPGGSSVPVLTAAECEKVTVDYDSLAAAGTMLGTAAIIVMDETTCMVQVA
                     SRLTRFYDDESCGQCTPCREGTAWAYKILNRLVHEGGEPHEIDLLLDICDNMGGRTIC
                     ALADAAVGPIKSCIEKFRPEFEYAVRHGSPMKGRMADVPGFELDAVGARH"
     gene            3250..4266
                     /gene="nuoH"
                     /locus_tag="VF720_12560"
     CDS             3250..4266
                     /gene="nuoH"
                     /locus_tag="VF720_12560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX7880237.1"
                     /translation="MGVGLPLDAVLGVVIKCLVFFFVIITAVAYMVQFERKVIAHIQS
                     RIGPNRVGPLGLLQPAADALKLMSKETIFPRDADKLLYLMGPVFASAPAFLSYAVIPV
                     GPDVNLFGQKISLYLADLNVALLFLLAVASINVYGVFLAGWSSNNKYSLYGGLRSSAQ
                     MISYELGMGMALIPVVMMAGSFSLVSIVEAQSNLPFLLLNPLAYLMFLICGLAEINRI
                     PFDLPEAEGELVAGFHTEYSGMRFGLFFLGEYANMITTSAIAVLLFWGGWNGPMIDKF
                     PALGVLWFTLKLLPHLFFLVWTRGTLPRFRYDQLMSFGWKILLPVGLANILITGLILA
                     LLQR"
     gene            4324..4884
                     /locus_tag="VF720_12565"
     CDS             4324..4884
                     /locus_tag="VF720_12565"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX7880238.1"
                     /translation="MWIFFLLAFLAIAGALAVVLLPNPIHSALMLVFTLMATAGIFLT
                     LGAQFLAVIQVIVYAGAIMVLFVFVIMLLNMRSGEGGEDRLHRIGIGQSLGIGLASLL
                     LLQLGLLVMAGKPSLLKSIHPAADLEAQAGGHSQAIGLLLYGKYLLPFEVASVLLLVA
                     IIGAVALSRRHLVVGPGRDETTEEVP"
     gene            4881..5207
                     /gene="nuoK"
                     /locus_tag="VF720_12570"
     CDS             4881..5207
                     /gene="nuoK"
                     /locus_tag="VF720_12570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX7880239.1"
                     /translation="MNEPVLPMLVPVAWYQLLAAAMFAIGALGVLFRRNILVIFMCVE
                     LMLNAANIAFVSFARHLGSLDGQIIVFFVLAVAAAEVSVGLAIIIDVFRKRGTVDLAA
                     ANLLKG"
     gene            5217..7187
                     /gene="nuoL"
                     /locus_tag="VF720_12575"
     CDS             5217..7187
                     /gene="nuoL"
                     /locus_tag="VF720_12575"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX7880240.1"
                     /translation="MDYFWLIPFLPLLGFLINGIFQKWLNEKSAGLIASAAVGAAFVL
                     AVVSFFGLMSLPADHRVVHHTISSWIEAGRFNAPLAFLLDPLSMVMVLVITGVGFLIH
                     VYSIGYMHGDSGVKRFFTYLNLFTFAMLVLVLADNLLLLFVGWEGVGLCSYLLIGYWY
                     EKKSATDAGKKAFIVNRIGDFGFILATSLTFWALGTVNFTEIAERAPGVLEAGGGVIT
                     AITLLFFLGATGKSAQIPLYTWLPDAMEGPTPVSALIHAATMVTAGVYLLARTHVLYT
                     MAPLAMFVVLVIGMATAIFAGSIGLAQTDIKKVLAYSTVSQLGFMFAACGVGAFAAAI
                     FHLVTHAFFKALLFLGSGSVIHGLHGEQEMPKMGGLKSRMPTTFLVFVIGGLALSGVP
                     IFAGFFSKDEILWQVFSSGNVIPWLILAIAALFTAFYTTRAIILTFYGQPRDKHLHDH
                     AHESPAVMTVPLMILAALSTIGGFLWLPIVSGGQKMKEWLTPVLGDGSHAAAAHAVAE
                     PAMAGHAPDAAPAASEHALSMAEALKATTEHSAAESARVAADAAHGASEAVHAVAGHA
                     PGGAFHYPGLLPSFGHNTSLEIILILVSVAIALVGIFLASRLYAGRAEAAERLRQSWG
                     GLHRLLLNKYFVDELYDAAVVRPVKSASDFLW"
     gene            7239..7364
                     /locus_tag="VF720_12580"
     CDS             7239..7364
                     /locus_tag="VF720_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880241.1"
                     /translation="MRGTGDELRRWQTGLAQNYAVAIVIGTLLIVAFVLWGKWSL"
     gene            7394..>8526
                     /locus_tag="VF720_12585"
     CDS             7394..>8526
                     /locus_tag="VF720_12585"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX7880242.1"
                     /translation="MLGALALALTPRQGNAARNVAFGVALVDFLLSLKLWFDFDLSAN
                     GMQFVERMPWIQSLGIRYAIGVDGLSLMLILLTTFLSAIAILASFNSISERTRSYYAC
                     LLALETGMLGVFLSFDLFLFYVFWEVMLIPMALLIGVWGGKRRVYAAIKFFIYTMAGS
                     VLMLVAIIATVLIHRQSSGVLTFDLFQIYSGTIARPAQMWLFAAFALAFAIKVPLFPF
                     HTWLPDAHVEAPTAGSVILAGVLLKMGTYGYMRYCLPMFPEAVLAFSPWILGLSVIGI
                     IYGALVAMVQPDIKKLVAYSSVSHLGFVMLGIFALNFQGLQGGLIQMINHGLSTGALF
                     LLVGMIYDRRHTREISEFGGITRVMPVFAIFFMVTTLSSIGLPG"
BASE COUNT         1325 a         2870 c         2702 g         1629 t
ORIGIN      
        1 gttgctcaca actatatagg gcgggacgga gagctcgaac caaccgtcgg cgtggagcag
       61 ggtgtcggga ggggtggcag ccgccggtgg cgtccagcag cccaatccca tgagcgttgc
      121 gacggcaagt ccggcaattc cacgaggcaa tcgggcgacc atggccgcag gatacacctc
      181 cctgcgggtc gatccggccc gattgacacc ccccgatgcc cgacctatcc tcccttgcac
      241 cttttttcac aaagcagggc ggcggcactt cgggtgtcgc gccggcgtcg tctttacgat
      301 ggtggaacgg cgctggtaag gtgcgggccg ctttccgggt ccgccgattt tctttccccc
      361 gcgggacgac acgaaccgac acgatggcgc tcaattacct cccaatcctg gtcatgctgg
      421 tgctggcgat cggcttcccg atcttcacgc tggtgctgtc gtgggttttg tcgccccggc
      481 agccggatcg catcaaggac gcggcctacg agtgcggcgt cgatccggtg ggggatgcgc
      541 ggctacgcca ttcggtccgc ttctacgtcg tggcgatgct cttcatcgtg ttcgacctgg
      601 aaacggtgtt cctctacccg tgggccgtca gctacaagaa gctcggcatg ttcggcctga
      661 tcgagatggt gatcttcatg gccatcctcg tcatcggcta catatacgtg tggaagcgcg
      721 gggcgctgga ctggagcgag cagctcgagc cggccgacct cacccggtac agcatggatc
      781 gcaccatcgg cgggccccgg gcccagatgc tcgggatcag ctacgaatgg gacgagccgg
      841 tgcgccggaa ggcggggggc aagtgagccg ccccaccact cccggctacg cgcacggcac
      901 cggttatggc cagaagacgg ccatggacct ccagtccgtg ttcggtgacg ccgtttccaa
      961 ctggcgcgag ttccgggggg actggcacat caccgtcgcg cctgcacgcc tgcgtgacgt
     1021 ggcgtaccac ctgcgcgaca cgctgggtta ccagatgctc tcgagcatga gcggcgtcga
     1081 ttactacccc gacgaaccgc gcttcaaggt cgtctacatg ctcacccaca tggtcgagaa
     1141 gacgcagttc ctgttcgagg ccttcacagg cggcgagaac cccgaactcc cgacgctcac
     1201 cgacatctgg atggtggccg actggcagga gcgcgaggtg tacgacttct tcggcatcaa
     1261 gttcaccggc catcccgacc tgcgccgcgt gctgatgccg gaggatttcg aaggatttcc
     1321 cctgcggaag gacttcccgc tcctgggcgt ggacgtcggc ctgaaggccc gcagctagga
     1381 catacgaaag gtctccgatg ctgtccgaga aaacgaaaaa ggacatcctg gcgatcaagg
     1441 accggtaccc ggaccggcgc tcggccacgc tgcccgcgct gtacgcggcg caggaagacc
     1501 agcgctggct gtcgtccgag acgatccagg aagtggccga cctcctcgag atgccggtgc
     1561 gcatgctgca cgaggtggcc accttctacg tgatgttcga gacgaagccg gtcggcacgc
     1621 acctcgtgga agtgtgcgac aacatttcct gcgcgctgcg cggcggcgaa gagatcatcc
     1681 agtacctcga gaggaagtgg gggatcaagc ggggcggcac gacgccggac ggcaagttct
     1741 ctctgcgcgg ggccgagtgc ctgggcgcct gcggaacggc gccgtgcatc atgatcgacc
     1801 accgctactg ggagaacctc acgttcgccg agctcgacaa gcggctggcg gcgctgaccg
     1861 acgatccgca tcacaccagg ggcgatctgg ccgagccgcc gatcccggcg gggaagagct
     1921 gatggcgccg agtttcccgc tcatcctcac gaagaacatg catcgggcgg acagccacac
     1981 gctggcggcc tacgaggcgt ccgggggcta taaggcctgg aaaaagacct tggacgggct
     2041 gacgcccgac caagtaatag ccacggtcaa ggattcgggc ctgcgcggcc gcggcggcgc
     2101 cggcttcccg tgcggcatga agtggtcgtt cgtgccgcgc cagagcgaga agcccaagta
     2161 cctggcctgc aacgcggacg aatcggagcc cggcacgttc aaggaccgga tgctcatgac
     2221 gcgcgatccc cacatgctga tcgaggggat ggccatcgcc tcgttcgcca tcgactgcca
     2281 cctggccttc atctacatcc ggggcgagtt cgccatcgag gcggtcatcc tggaggcggc
     2341 catcgaggag gcccggcagg ccggacatct cggcaggaac atctacggca aggggtacga
     2401 tctcgacatc ctggtgtacc gcggggcggg cgcctacatc gcaggcgaag aaaccgggat
     2461 gctcgagagc ctggaaggga agcgggcgca cccgcgcatc aagcccccct tcccggccgt
     2521 ggtgggcttg tacggcgcgc ccacggtgat caacaacgtg gagacgctgg ccaacgtgcc
     2581 ggccatcatc gagaacggcg ccgactggtt caaggcgatc ggcacgccca agagtaccgg
     2641 cacgaagctg atgaacatct ccgggcacgt gaaccggccg ggcgtggtgg agctgccgat
     2701 gggcacgccg ctgatgacga tcatcaacga ctactgcggc ggcgtgtggc acaaccgcaa
     2761 gctcaaggcg gtgatccccg gcggctcgtc cgtgccggtg ctcacggccg ccgagtgcga
     2821 gaaggtgacc gtcgattacg acagcctggc cgccgccggc acgatgctcg gcaccgcggc
     2881 catcatcgtg atggacgaaa cgacctgcat ggtccaggtg gcctcgcgcc tcacgcgctt
     2941 ctacgacgac gaatcgtgcg gccagtgcac gccgtgccgt gagggcaccg cctgggcgta
     3001 caagatcctg aaccgcctgg tgcacgaggg gggcgagccg cacgagatcg acctcctcct
     3061 cgacatctgc gacaacatgg gcggccgcac catctgcgcc ctggccgacg cggccgtggg
     3121 ccccatcaag agctgcatcg agaagttccg ccccgaattc gagtatgccg tgcgccacgg
     3181 atcgcccatg aaggggcgga tggccgatgt gccggggttt gagttggacg ccgtcggagc
     3241 gaggcactga tgggtgtcgg actgccgctc gatgccgtgc tcggcgtcgt catcaagtgc
     3301 ctggtcttct tcttcgtcat catcaccgcc gtggcctaca tggtgcagtt cgagcggaag
     3361 gtgatcgccc atatccagag ccgcatcggg ccgaaccggg tggggccgct cggcctcctg
     3421 cagccggcgg ccgacgcgct caagctgatg tcgaaggaaa ccatcttccc gcgcgacgcg
     3481 gacaagcttc tttacctgat gggaccggtc tttgcctcgg cgccggcctt cctgtcgtac
     3541 gcggtcatcc cggtcgggcc ggatgtgaac ctcttcggcc agaagatctc gctttacctg
     3601 gccgacctga acgtggcgct cctgttcctc ctggccgtgg cgtcgatcaa cgtgtacggc
     3661 gtcttcctgg ccgggtggtc gtcgaacaac aagtactcgc tgtacggcgg cctgcgctcc
     3721 agcgcccaga tgatttcgta cgagctgggg atggggatgg cgctgatccc ggtggtcatg
     3781 atggccggga gcttcagcct ggtgagcatc gtggaagcgc agtcgaacct gccgttcctg
     3841 ctcctcaatc cactggccta cctgatgttc ctgatctgcg gcctggccga gatcaaccgc
     3901 attccgttcg acctccccga agccgagggt gagctggtgg ccgggttcca caccgagtat
     3961 tcgggcatgc gcttcggcct tttcttcctg ggcgagtacg ccaacatgat caccacctcg
     4021 gccatcgccg tgctcctctt ctggggcggc tggaacgggc cgatgatcga caagtttccg
     4081 gcgctcggcg tcctctggtt cacgctgaag ctcctgcccc atctcttttt cctggtgtgg
     4141 acgcgtggca cactgccgcg cttccgttac gaccagctca tgagcttcgg ctggaagatc
     4201 ctcctgccgg tcgggctggc caacatcctg atcaccggac tgatcctggc gctcctgcaa
     4261 cgctgaggcg atgcgggcgc gtcatcgaca cgatttgacg caacaacgag ggcggacctg
     4321 ctgatgtgga ttttcttcct gctggccttc ctggcgatcg ccggggcgct ggcggtggtg
     4381 ctcctgccga acccgatcca ttcggcgctg atgctcgtct tcacgctgat ggccacggcg
     4441 ggcatcttcc tgacgctggg ggctcagttc ctggccgtca tccaggtgat cgtgtacgcc
     4501 ggcgccatca tggtgctgtt cgtcttcgtg atcatgctgc tcaacatgag atcgggcgaa
     4561 gggggcgaag atcgcctcca ccggatcggc atcgggcaga gcctggggat cggcctcgcc
     4621 tcgctgcttc tgctccagct cggcctgctc gtcatggccg gcaagccttc tctcctcaag
     4681 tccatccacc cggcggcgga tctcgaagcc caggccgggg gacacagcca ggccatcggc
     4741 ctgttgctct acggcaagta tctcctgccg ttcgaagtgg cctcggtgct cctcctggtg
     4801 gccatcatcg gcgcggtagc gctctcccgc cgccacctgg tggttggccc gggccgcgac
     4861 gagacgaccg aggaggtgcc atgaacgaac ccgtcctgcc gatgctcgtg ccggtggcct
     4921 ggtaccagct gctggccgcg gccatgttcg ccatcggcgc gctgggcgtg ctcttccggc
     4981 gcaacatcct ggtcatcttc atgtgcgttg agctgatgct caacgcggcc aacatcgcct
     5041 tcgtgtcgtt cgcacgccac ctggggtcac tggacggcca gatcatcgtc ttcttcgtcc
     5101 tggcggtggc ggcggccgag gtatcggtcg gcctggccat catcatcgat gtcttccgga
     5161 agcgcggcac ggtcgacctg gccgccgcga acctcctgaa aggctgaccg gaacgcatgg
     5221 attacttctg gctcatcccg ttcctgccgc ttctcgggtt cctgatcaac ggaatcttcc
     5281 agaagtggct gaacgagaaa tccgccgggc tgatcgcctc ggccgccgtc ggtgcggcgt
     5341 tcgtcctggc ggtggtcagc ttcttcggcc tcatgtcgct tccggccgac caccgggtgg
     5401 tgcaccacac catctcgagc tggatcgagg ccggccggtt caacgcgccg ctggccttcc
     5461 tgctcgaccc gctttccatg gtgatggtgc tcgtcatcac cggagtgggc ttcctgatcc
     5521 acgtctattc catcgggtac atgcacggcg attcgggcgt gaagcgcttc ttcacgtacc
     5581 tgaacctctt cactttcgcc atgctcgtgc tggtgctggc cgacaacctc ctgctcctgt
     5641 tcgtcggctg ggaaggggtg gggctctgtt catacctgct gatcggctac tggtacgaga
     5701 agaagtcggc caccgacgcc gggaagaagg cgttcatcgt caaccggatc ggtgacttcg
     5761 gcttcatcct ggccacgtcg ctcacgttct gggcgctggg caccgtcaac ttcaccgaga
     5821 tcgccgaacg ggccccgggc gtcctcgagg cgggcggagg agtgatcacc gccatcacgc
     5881 tcctcttctt ccttggggcc accggcaagt cggcccagat cccgctctac acgtggctgc
     5941 ccgacgccat ggaaggcccg acgccggtgt cggcgctcat ccacgccgcc acgatggtga
     6001 cggccggcgt gtacctgctg gcccgcacgc atgtgctcta cacgatggcg ccgctggcga
     6061 tgttcgtggt cctcgtcatc gggatggcca cggccatctt cgccggctcg atcggcctgg
     6121 cccagaccga catcaagaag gtgctggcct actccacggt gagccagctc ggcttcatgt
     6181 tcgccgcctg cggcgtgggg gccttcgcgg cggccatctt ccacctggtc actcacgcct
     6241 tcttcaaggc gctcctcttc ctcggctcgg gctcggtgat ccacggactg cacggcgagc
     6301 aggagatgcc gaagatgggc ggcctcaagt ccaggatgcc caccaccttc ctggtgttcg
     6361 tcatcggcgg cctggccctc tccggcgtgc cgatctttgc gggcttcttt tccaaggacg
     6421 agattctctg gcaggtcttc tcgagcggca acgtcatccc gtggctcatc ctggccatcg
     6481 cggcgctgtt caccgccttc tacacgacac gggccatcat cctcacgttc tacggccagc
     6541 cgcgcgacaa gcacctccac gaccacgcgc acgaatcgcc cgccgtgatg accgtgccgc
     6601 tcatgatcct ggcggccctc tcgacgatcg gcggtttcct ctggctcccg atcgtttcag
     6661 gcggccagaa gatgaaggaa tggctcacgc cggtcctggg agatggttcg cacgcggccg
     6721 ccgcacatgc cgtggcggaa cccgccatgg ccgggcacgc ccccgacgcg gcgccggcgg
     6781 ccagtgagca tgcgctctcc atggccgagg cgctcaaggc caccaccgaa cattcggctg
     6841 ccgaatcggc ccgggtggcg gccgacgcgg cccacggggc gtccgaggcg gttcacgcgg
     6901 tggcggggca tgctccgggt ggcgcgttcc attatcccgg cctcctcccg tcgttcggcc
     6961 acaacacgtc gctcgaaatc atcctgatcc tggtgtcggt ggcgatcgcg ctcgtcggca
     7021 tcttcctggc ctcgcgcctg tacgccgggc gcgccgaggc cgccgaacgc ctccgacaga
     7081 gctggggcgg cctgcaccgg ctccttctca acaaatattt cgtcgacgag ctgtacgacg
     7141 cggccgtggt ccggccggtc aagtcggcct ccgatttcct gtggtaggtc gtcgacgcgc
     7201 gcggcatcga cggcgccgtc aatggcgtcg gcacgttcat gcgcggcacc ggcgacgaac
     7261 tccgtcgctg gcagaccggg ctggcccaga actacgcggt ggccatcgtc atcggcaccc
     7321 tgttgatcgt ggccttcgtc ctgtggggga agtggtcgct gtgagctatc tctcgatcgt
     7381 cctcttcctc cccatgctcg gcgctctggc gctggcgctg acaccccggc aggggaacgc
     7441 ggcccgcaac gtggccttcg gcgtggccct cgtcgacttc ctgctgagcc tgaagctctg
     7501 gttcgacttc gacctgagcg ccaacggcat gcagttcgtg gaacgcatgc cgtggatcca
     7561 gtcattgggg atccgctacg ccatcggcgt ggacggcctt tcactcatgc tcatcctgct
     7621 caccacgttc ctgtcggcca tcgccatcct ggcgtcgttc aactccatca gcgaacggac
     7681 ccgctcgtac tacgcctgcc tcctggccct cgagaccggg atgctcggcg tcttcctgtc
     7741 gttcgacctc ttcctcttct acgttttctg ggaagtgatg ctgatcccga tggcgctcct
     7801 gatcggcgtg tggggcggca agcggcgcgt gtacgcggcc atcaagttct tcatctacac
     7861 gatggcgggg tcggtgctca tgctcgtggc catcatcgcc acggtgctca tccaccgcca
     7921 gtcgagcgga gtcctgacgt tcgacctctt ccagatctat tcgggcacca ttgccaggcc
     7981 ggcgcagatg tggctcttcg ccgccttcgc gctggccttc gccatcaagg tgccgctctt
     8041 cccgttccat acctggctcc ccgacgcgca cgtggaggcc ccgacggccg gctcggtgat
     8101 cctcgccggc gtgctcctca agatgggcac ctacggttac atgcgttact gcctgccgat
     8161 gttccccgag gcggtcctgg cgttctcccc ctggatcctc ggcctgtcgg tgatcggcat
     8221 catctacggg gctctcgtgg ccatggtcca gcccgacatc aagaagctgg tggcctactc
     8281 ctcggtgagc cacctcgggt tcgtcatgct cgggatcttc gcgttgaatt tccaggggct
     8341 gcagggcggt ctgatccaga tgatcaacca tggcctctcg accggcgccc tcttcctcct
     8401 ggtcggaatg atctatgacc gccgccacac gcgcgagatc tccgagttcg gcggcatcac
     8461 gcgcgtcatg cccgtcttcg ccatcttctt catggtcacc actctctcct cgatcggcct
     8521 gcccgg
//
LOCUS       DASVOD010000571         6366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_766979, whole genome shotgun sequence.
ACCESSION   DASVOD010000571 DASVOD010000000
VERSION     DASVOD010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766979"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF720_12590"
     CDS             complement(<1..260)
                     /locus_tag="VF720_12590"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX7880243.1"
                     /translation="MPELPDVLLYVDHLSRLLVGRPLAVVRILGPSVLQTWDPPVSTL
                     TGKQVESVSRIGKRLVWHLEDDLHVVIHPMVAGRFHLRKPDAA"
     gene            complement(265..2517)
                     /locus_tag="VF720_12595"
     CDS             complement(265..2517)
                     /locus_tag="VF720_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007325977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX7880244.1"
                     /translation="MDCAEEVAILKREIGPLVGGADRLAFDLINARMIVGSAPAVEPG
                     PEGGNASAGGHAPAAGSAAQVPIDDEAIIRAVARTGMSAERWRPEAVGQNQRDDRARR
                     RTTTVSGVAALVGILVMASEGGWRAVLAHEAADAKGTPLLAIVTFLVAVAASFWLVVP
                     RAWHALTRRAPDMNLLMTLAVIGAIALGEWSEAGVVAFLFSLSLELESWSLGRARKAV
                     QALLDLAPPSARRLDAQGNESQVAPDMLVVGDRIAVRPGERIPIDGAILTGVGSVNQA
                     PITGESLPVPKMPGDEVFAGSINEDGALTIVVNKSFEETTLRQIIRQVDEAHARRSPA
                     ERWVDSFARVYTPVILVIATAVAVAPPLLFAGAWSDWIYRGLVLLVIGCPCALVISTP
                     VCVVAALARAARNGVLIKGGAALEAPARLTAMAFDKTGTLTEGRLTLAEVVPLNGHDK
                     TELLSRAAALESSSNHPLAIAVINGARRQGVVPAIGGAHQVVPGKGVHGTFEGRDYWL
                     GSHLYLEERGQETPEVHDRLEALGADGHSVVVIGNDRHVCGFLTAVDRVRPEAKAALA
                     ELHELGIRDIALLTGDNRGTATAIGHAVGIDEVMADLKPADKVASVAALVQRHGQVAM
                     VGDGINDAPALARASLGIAMGAAGSDAAIETADVALMSDDLRKIPWLVRHSRRTLSVI
                     RANVTLAIGIKAAFLALTLAGKASLWAAIAADMGVSFLVIANGLTLLGEPRTFTIGKD
                     VRTDPVAGVP"
     gene            2603..4138
                     /locus_tag="VF720_12600"
     CDS             2603..4138
                     /locus_tag="VF720_12600"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEX7880245.1"
                     /translation="MTAEPVVGLDFGSIRGRLTAAGDVRVFRGVPFAADTGGANRWRG
                     PQPVRAWSGIRDALAPGVICPQPKAIIWPIKDPQGEDCLTLSVWAPAGEPESNSGRWP
                     ILLWIHGGGFSTGAGSTIFFDGEELARLGAVVVTINYRLGPFGFLAHPAFSAESPDKV
                     SGNVGLLDQVAALEWVQRNGAAFGGDPECVTVFGESAGAASIARLLVSPKAERLFHRA
                     ILQSGHARGRNRWLRKEVPGFEAAETVGTRIAAALGCSDVGPVAETACLRAKTPAEVL
                     VAADPAQGLYGGGIKFAPIVDGVIIPDDPERLFAAGRFHRVPILIGTNADEGSIFTGA
                     TRMTTRAQFDGMLRRRFGPLADEAARLYPVAADADVEPALNRLITDASFLTPARALAR
                     QVATVQPDKVWMYHFTRVSPAGRKNGRGAFHAAEVSYVFNRVGNTLAYDDTDRAVARA
                     MSRTWVEFARSGRANWSPYSPDTESYLDIGDTLSERQHLRREACDLIDRVAAARGSDG
                     EGE"
     gene            complement(4202..4627)
                     /locus_tag="VF720_12605"
     CDS             complement(4202..4627)
                     /locus_tag="VF720_12605"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7880246.1"
                     /translation="MTRLVIDASVWVAALDASDEFSAPSRAFLDAATRQRVSLEIPAH
                     APIEIACALARRLGNARVARELGGNLVRMPGIRLHALTAELLERAGFLGTESMLRSGD
                     AIYAALAQQIEARVITWDGDLHDRAEGVTPTEWMATEAG"
     gene            complement(4624..4890)
                     /locus_tag="VF720_12610"
     CDS             complement(4624..4890)
                     /locus_tag="VF720_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880247.1"
                     /translation="MKATFDLPDDLYKRVKARSARAGRPVRDVVIELFEGWLDDDAES
                     AAAVASDQWLRSWFAEADACFRPAAKGPNARTILRSDRDRLDRK"
     gene            5021..5368
                     /locus_tag="VF720_12615"
     CDS             5021..5368
                     /locus_tag="VF720_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7880248.1"
                     /translation="MSNPEKITLRKVKDILIVQLPAALSADTKEDFDEKVEYALADTP
                     GVLILDFAKVTSADAAGVKSLDFAFMRCEEIDTPLIMAAVADSIRPALEAAGYLKRGE
                     ESATADEAVQANL"
     gene            complement(5377..6183)
                     /locus_tag="VF720_12620"
     CDS             complement(5377..6183)
                     /locus_tag="VF720_12620"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7880249.1"
                     /translation="MSPESLPNNLSFPEMYERYLVEALFRPFAEGLLDRLAPSPGTAH
                     LDVACGTGIVARVMRQRLGPNATVVGVDTSPGMLDVARRVAPDIDWRVGDAKSLPLND
                     GEAFDIVTCHQGLQFMPDKPAAIAEMVRALVSGGRLGVSCWRSHRETPLFRDVYDVAE
                     RHLGPIVDHRYGFGEIEPLRALLAGAGLNGIQVETEGRACRFEDGSFFVRLNTMALVG
                     MSAAGKAMSEEERARTTEAIIADTVPVAARHMEGKALVFEALTNVATGRK"
BASE COUNT          995 a         2190 c         2181 g         1000 t
ORIGIN      
        1 gcggcatccg gctttcgcag gtggaaacgt ccggcaacca tgggatgaat gacaacgtgc
       61 agatcgtcct cgagatgcca gaccagccgc ttgcctattc gcgaaacact ctcgacctgt
      121 ttgccggtca gggtcgacac cggcggatcc caggtctgaa gcaccgacgg accgagtatg
      181 cgcaccaccg cgagaggtcg gcccacgagc agccgcgaca ggtgatcgac atacaggagc
      241 acgtccggca gctcgggcat ggaatcaggg gacgcccgcc acggggtcgg tgcggacgtc
      301 cttgccgatc gtgaacgtgc gcggctcgcc gagcagcgtg agcccgttgg cgatcaccag
      361 gaagctaacc cccatgtcgg ccgcgatggc ggcccagagc gacgccttgc cggccagcgt
      421 gagcgccagg aaggccgcct tgataccgat ggcgagcgtg acgttggcgc ggatcaccga
      481 aagggtgcgc cgcgagtggc gcacgagcca ggggatcttg cggaggtcgt ccgacatcag
      541 cgccacgtcg gcggtctcga tggcggcatc cgaaccggcg gcgcccatcg cgatccccag
      601 gctggcccgg gccagcgccg gcgcgtcgtt gatgccgtcc cccaccatcg ccacctggcc
      661 gtggcgctgc acgagcgcgg ccacggaagc caccttgtcg gccggtttca ggtcggccat
      721 cacttcgtcg atgccgaccg cgtggccgat ggcggtggcg gttccccggt tgtcgccggt
      781 gagcagggcg atgtcgcgga tcccgagctc gtggagctca gccagtgccg ccttcgcctc
      841 gggcctcacg cgatcgaccg cggtcaggaa tccgcacacg tggcgatcgt tgccgatgac
      901 gacgaccgag tgaccgtcgg cgccgagtgc ctcgagacga tcgtgcacct cgggcgtttc
      961 ctggccccgt tcctcgagat agagatgcga gccgagccag taatcgcgtc cctcgaacgt
     1021 gccgtgcact cccttgccgg gcacgacctg gtgcgcgccg ccgatggccg gcaccacgcc
     1081 ctgccgtcgc gcaccgttga tgaccgcgat ggccagcggg tggttgctcg acgactcgag
     1141 cgccgccgcg cgcgagagga gctcggtctt gtcgtgcccg ttcaacggca cgacctcggc
     1201 cagcgtcagt cgcccttcgg tgagcgtgcc ggtcttgtcg aacgccatcg ccgtgaggcg
     1261 tgccggcgct tccagcgcag cgcccccttt gatgagcacg ccgttccgcg ccgcgcgggc
     1321 caacgccgcc accacgcaga ccggcgtgga gatgaccagc gcgcacgggc agccgatcac
     1381 gaggagcacg agtccgcggt agatccagtc gctccaggcg ccggcgaaca ggagcggcgg
     1441 cgccaccgcc accgcggtgg cgatcacgag gattaccggc gtgtagacgc gggcgaagct
     1501 gtccacccag cgctcggccg gcgagcgacg cgcatgcgcc tcatccacct ggcggatgat
     1561 ctggcgaagc gtggtttcct cgaacgactt gttgacgacg atggtcagcg cgccgtcttc
     1621 gttgatcgaa ccggcaaaga cctcatcgcc cggcatcttt ggcacgggca agctctcccc
     1681 ggtgatcggc gcctggttca cggaaccgac gccggtcagg atggcgccgt cgattggaat
     1741 gcgctcgccc gggcgcacgg cgatgcgatc gcccacgacc agcatgtccg gcgccacttg
     1801 tgattcgttc ccctgtgcgt cgagtcgccg ggccgagggt ggcgcgaggt cgagaagcgc
     1861 ctgcaccgct ttccgcgcgc gaccgaggct ccatgattcg agttcgagcg acagcgagaa
     1921 gaggaaggcc acgacacccg cctcggacca ttcgccgagc gcgatggcgc cgatcacggc
     1981 gagtgtcatg aggaggttca tgtcgggcgc gcgccgcgtg agggcatgcc acgctcgcgg
     2041 cacgaccagc cagaaggatg cggcgacggc caccaggaag gtgacgatgg cgaggagtgg
     2101 cgtgcccttg gcgtccgcgg cttcgtgggc cagcacggcg cgccagcccc cctcggaggc
     2161 catgaccagg atgccgacca gtgccgccac gccgctgacg gtggtggtgc ggcgccgggc
     2221 gcggtcgtcg cgctgattct gcccgacggc ctcgggacgc cagcgttcgg ccgacatgcc
     2281 ggtcctggcc accgcccgga tgatggcttc gtcgtcgatc gggacctggg cggcgctccc
     2341 ggcggccggg gcgtgtccgc cggcggatgc gttcccaccc tccggcccgg gctccactgc
     2401 cggcgcgctc cccacgatca tccgcgcgtt gatcaggtcg aacgccagtc gatcggcccc
     2461 cccgacgagc ggtccgatct cgcgcttcag gatggccact tcctcggcgc agtccattcc
     2521 ccgtatcttg aacgccgatg acgacgcagc gggtggtgcc attccgaaag cgtagcaggg
     2581 agacggcggg agcgtcgccc cgatgacggc tgagccggtt gtcgggctcg atttcggcag
     2641 catccgtggc cggctgacgg ccgccggaga tgtccgggtg ttccgcggcg tcccgttcgc
     2701 ggccgacacc ggtggcgcca accgctggcg tgggccgcaa ccggtgcgcg cgtggtcggg
     2761 gatccgcgat gcgctggcac cgggggtgat ctgcccccag ccgaaggcga tcatctggcc
     2821 gatcaaggac ccgcagggcg aagattgttt gacactcagc gtgtgggcgc ccgccggcga
     2881 acctgagtcg aattcaggcc gctggccgat cctgttatgg attcatggtg gcggcttctc
     2941 gaccggcgcc gggtcgacga tcttcttcga cggcgaggaa ctggcgcgac tgggcgctgt
     3001 cgtggtcacg atcaactatc gactggggcc cttcgggttc cttgcccatc ccgcgttctc
     3061 cgccgaatcg cccgacaagg tgtccggcaa tgtcggcctg ctcgaccagg tggccgcgct
     3121 cgaatgggtc cagcgcaacg gagccgcctt cggcggcgat cctgagtgcg tcacagtatt
     3181 cggcgagtcg gcgggtgcgg ccagcatcgc ccggttgctg gtgtccccga aggcggagcg
     3241 gctgttccac cgggccatcc tgcagagcgg ccacgcccgg ggtcggaacc gctggctccg
     3301 gaaggaggtg cccgggttcg aggcggccga gacggtcggc acgcgaatcg ccgcggcact
     3361 tggctgcagc gacgttggcc ctgttgccga aacagcctgc ctgcgcgcga agacgcccgc
     3421 cgaggtcctt gtcgccgccg atccggccca ggggctgtac ggcggcggca tcaagttcgc
     3481 cccgatcgtc gacggcgtca tcattcccga cgatcccgaa aggctgtttg ccgccggtcg
     3541 cttccaccgc gtgccgatcc tgatcggcac caacgccgac gaaggatcga tcttcaccgg
     3601 tgcgacgcgc atgaccacca gggcacagtt cgacggcatg ctgcgccggc ggttcggccc
     3661 cctggcagac gaggccgcgc ggctttaccc ggttgcggct gacgccgatg tcgagccggc
     3721 gctcaaccgc ctcatcaccg atgcctcgtt cctgaccccc gcccgcgcgc tggcccgaca
     3781 ggtggcgacg gtgcaacccg acaaggtctg gatgtaccac ttcacccgtg tttcccccgc
     3841 cgggcggaag aacggccgtg gcgctttcca cgccgcggag gtctcgtacg tcttcaaccg
     3901 cgtcggcaac acgctggctt acgacgacac cgatcgcgcc gtggcgcgcg cgatgtcccg
     3961 tacgtgggtc gagttcgccc ggagcggccg tgcgaactgg tcgccataca gtcctgacac
     4021 cgaatcgtac ttagacatcg gcgacaccct gagtgaacgt cagcatctgc gccgggaggc
     4081 ctgcgacctg atcgaccggg tggcggcagc acggggatcg gatggcgagg gggagtgacc
     4141 ggaaccgctc cggatgacgc gggacgggga ataccgggcg agctgagcgg cgcaccggcg
     4201 gtcagccagc ctcggtagcc atccactctg ttggcgtcac gccctcggcg cgatcgtgca
     4261 ggtccccatc ccaggtgatc acccgggcct cgatctgttg cgcgagcgcc gcgtagatgg
     4321 cgtcgccgga gcggagcatt gattcggtgc ccagaaaccc ggcacgctcg agaagctcgg
     4381 cggtgagggc gtggagccgg atcccaggca tgcggacgag atttccaccg agctcgcggg
     4441 caacccgcgc gttcccgagt cggcgggcca gggcacaggc gatctcgatc ggtgcgtggg
     4501 ccgggatctc cagcgacacg cgttggcgag tggcggcatc gaggaaggcg cgactgggcg
     4561 ccgagaattc atcggaggca tcgagcgccg ccacccagac gctggcgtcg atgaccaggc
     4621 gtgtcatttc cgatcgaggc gatcgcggtc cgaacggagg atcgtgcgcg cgttcgggcc
     4681 tttcgcggcg ggccggaagc aggcgtcggc ctcggcgaac caggagcgca gccactggtc
     4741 cgaggcgacg gccgccgcgc tctcggcgtc atcgtcgagc cacccctcga agagctcgat
     4801 cacgacgtcc cgcaccggac gtccggctcg tgccgatcgc gcctttaccc gcttgtagag
     4861 gtcgtcgggc agatcaaacg tggccttcat tcatacagct tcctgtattc ctgtacgacc
     4921 gtcaaggaga ttctccatcc ccctatcttc ttgcggcacc cttggatggc ttagactggc
     4981 ggaccggatt cgtcatgccc cttcaccgcg gagacccgcc atgtccaacc ccgaaaagat
     5041 caccctgcgc aaggtgaagg acatcctgat cgtccagctc cccgccgccc tttcggccga
     5101 caccaaggaa gatttcgacg agaaggtgga gtacgccctg gccgacacgc cgggcgtgct
     5161 gatcctcgac ttcgcgaagg tcacctccgc ggatgcggcc ggcgtaaagt ctctcgactt
     5221 cgccttcatg cgctgcgagg agatcgatac cccgctcatc atggcggcgg tggccgattc
     5281 gattcgcccc gcgctcgaag cggcgggata cctgaagcgc ggcgaggaat ccgccacggc
     5341 ggacgaagcg gtccaggcga acttgtagtt ccgaccctat ttccttccgg tggccacgtt
     5401 ggtcagcgcc tcgaacacga gggccttccc ctccatgtgg cgcgccgcaa ccggcaccgt
     5461 gtcggcgatg atcgcctcgg ttgtccgggc gcgctcctcc tcgctcatcg ccttgccggc
     5521 ggcactcatg ccgaccagcg ccatggtgtt gaggcggaca aagaacgagc cgtcctcgaa
     5581 ccggcacgcg cgcccttccg tctcgacctg tatcccgttc agtccggcgc cggcgagcag
     5641 cgcccgcagg ggttcaatct cgccgaaccc gtagcgatgg tcgacgatcg gccccaggtg
     5701 ccgctcggcc acgtcataca cgtcgcgaaa cagcggcgtc tcgcgatgcg agcgccagca
     5761 cgacaccccg aggcgccccc cggaaacgag ggcgcgaacc atttcggcga tggcggccgg
     5821 cttgtcgggc atgaactgca ggccctggtg gcaggtcacg atgtcgaagg cctcgccgtc
     5881 gttgagcggg agcgacttcg catcgccgac gcgccagtcg atgtccggcg cgacccggcg
     5941 ggccacgtcc agcatccccg ggctggtatc gaccccgacg acggtggcgt tggggccgag
     6001 ccgttgacgc atgacgcggg ccacgatgcc ggtaccgcag gccacgtcga ggtgggccgt
     6061 gccgggcgac ggtgcgagtc gatcgagcag accttcggcg aagggtcgaa agagcgcttc
     6121 gacgaggtag cgctcgtaca tttcgggaaa gctgagattg ttcggaagac tctcggggct
     6181 catcgggcct ccttcaccgc tccacaccgc cattcttcag ctccacgccg atcacgtgca
     6241 ggtcgcgatc gcccacattt tccagcgagt gaggcggaag cgccggcaac caggcggagt
     6301 ccgggggcgg cacttgcccc ggcggtccgg tccggctgtc gaaattgatc gtgccgttgg
     6361 catcgc
//
LOCUS       DASVOD010000572         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_714162, whole genome shotgun sequence.
ACCESSION   DASVOD010000572 DASVOD010000000
VERSION     DASVOD010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714162"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VF720_12625"
     CDS             complement(<1..596)
                     /locus_tag="VF720_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880250.1"
                     /translation="MNGWPPARFRWAALAAALVAALTTTGLTMLLFSPIATSGAADDP
                     GITPLAAFLRIGAATVAGVLAGSLAGWLMARRELATLSRLVPLLQEGLPQADDSGGSA
                     DQPLPSLLPMAEMVAEAARRGREASGQLARLESALSRLVRSLGSAADSDVTLPAVGPE
                     ASSAEAALGRAVQALVETSQKTRDRTGTLLAEVEDHLLP"
     gene            848..1720
                     /locus_tag="VF720_12630"
     CDS             848..1720
                     /locus_tag="VF720_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880251.1"
                     /translation="MESSRQLFVDMKDDTRRLRPSYVFMMRCLLYSRLETGNETGSDV
                     PDEDVNVYLAHLLQSFSDPDYVEGARPYLHRYDHEVFKRLERSTDARLKYVIYKTNAD
                     FLLVSIGVFDNPGQWLAHRLPQVQPSKPRTGEPSEEASMGRGKTYYHFAWSYSQQVNR
                     GHPGITEVLEKLSVGFDRYIRVLSHMRGEYLDLMKALSTGEIYHLERNINKEQERQQL
                     KESQDAFLDSWLAWKRSGAPETLEELRRHAAAIRAVDPEFQFSEDDTAPEIPKLAPEP
                     VRPARSPARQRKPI"
     gene            complement(2062..2274)
                     /locus_tag="VF720_12635"
     CDS             complement(2062..2274)
                     /locus_tag="VF720_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880252.1"
                     /translation="MKTFGLILIGLGIIGLLYGGITYNKKEKVLDIGELEASVTKKER
                     VSFPPLAGGAAIVVGALLILVDRKPR"
     gene            complement(2335..2484)
                     /locus_tag="VF720_12640"
     CDS             complement(2335..2484)
                     /locus_tag="VF720_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX7880253.1"
                     /translation="MLYTIAVVLLLLWLLGLVTSYTMGGFIHILLVLAVVSILLRVIS
                     GRRPV"
     gene            complement(2730..5144)
                     /locus_tag="VF720_12645"
     CDS             complement(2730..5144)
                     /locus_tag="VF720_12645"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7880254.1"
                     /translation="MNATNAPLPRPLLFGIFFISMGMLLQEIILTRIFSVTMFYHFAF
                     MVVSITLFGIGVSGIWVYLNPKRFSRERAPEQMARSALLFAIAVPLCFILQRSLPAAA
                     DSPRWGLLYLAATYVLIAIPFFLGGTAISLALTHWSGGVSTLYFADLVGASIGCALVF
                     PGLTFLTGPDIVILVGVIGALSAVAFAQLAGPSERRLAWFTAAAIGALLVVNVATPFK
                     PVNVGSRAIQRPDSQEWVEPLAAKWNPMARVLAFNLSNRPVFRPGHSPDSTFFPDDQI
                     LFQLDRSAMTWAVPFSGDWSAVDWIYTDVSVLPLFLKPGGKLLDIGSGGGQNALAAIG
                     LGSKDVTCVEINPTVVEWTKTKFDDITGHIYTRPEVRVVVGDGRTVTARSTETYDVIA
                     FVSSTTFTATTSGAFMMAENNLFTREAFQEYWKHLTPDGMMMASQVMTSDYPGLMLRM
                     TGLARATLSANGVADAARHIIVITKQNEGYGAMVMKKSAFTDAELDILDGKAREMGWK
                     VLHSPRVSNHPQFHELITTPDFNEWVNAQPLNLYPPTDDKPFFFNLVPFSRLNQTRAG
                     NYDIKYGKVPAQILLNLFFIVTVLTVLFILVPLFLARRARFEQKPGTLPTLLFFTMIG
                     LGFIMIEVPLMQRFVLYLGHPIYAITTILFSILLFSGVGSYLTRFMAPGTSLGWLKRS
                     IPALLVLTVVYNLGLEKVFAATQNQSQGVKVAVSMLLLLPLGLLMGMPFPVMMRRVSA
                     WSPGTIPWCWGVNGATSVLGSVSGVIVALASGFTASLWVGMAAYLGAFFCTLAIRDAG
                     PHSKSA"
     gene            complement(5211..>6116)
                     /locus_tag="VF720_12650"
     CDS             complement(5211..>6116)
                     /locus_tag="VF720_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX7880255.1"
                     /translation="EKNVLKGLITVKDIQKKLQYPNATKDSHGRLRVAAAIGVAGDAV
                     ERVAHLTSQHADAIVLDSAHGHSEGVLSLVKQIRTAHPGVQLIVGNVATKRAAEDLIG
                     LGVDAIKVGMGPGASCTTRVVAGIGVPQITAIMECVAAAAPSGVPVIADGGIRYSGDI
                     VKALASGASTVMIGALFAGTEESPGEIILLEGRSYKVFRGMGSMAAMQEGSADRYFQG
                     GVTDRRKLVSEGIEGRVPYKGPLTHLVFQMVGGLRAGMGYVGVDNVPDLATKSRFIKV
                     TAAGLRESHPHDVAITKEPPNYEVR"
BASE COUNT         1144 a         2022 c         2009 g          941 t
ORIGIN      
        1 ggcaggagat gatcttccac ttcggccagg agcgtgcccg tccggtctcg ggtcttctgt
       61 gacgtctcga ccagtgcctg cacggcccgt ccgagcgccg cttcggcgct ggatgcctcg
      121 ggtccgacgg ccggcaacgt cacgtcgctg tcggccgccg atccgagcga tcggaccagt
      181 cgggacaacg ccgattcgag ccgagccagc tggcccgacg cctcgcggcc ccgccgcgcc
      241 gcttccgcga ccatctcggc catcggaagc agcgaaggga gaggttgatc cgccgatcca
      301 ccggagtcat cggcctgtgg gagcccttcc tgaagtaaag ggacgagccg ggagagcgtg
      361 gccaactcgc gacgcgccat gagccagccg gccagggatc cggcgaggac cccggccacc
      421 gtggccgcgc cgatgcgcag gaaggcggcc aggggagtga tgccgggatc atcggcggcg
      481 ccgctcgtgg caatgggcga gaagagaagc atggtgaggc ccgtcgtggt cagcgccgcg
      541 acgagggccg ccgcgagcgc cgcccagcgg aaccgggcgg gaggccatcc gttcatgagt
      601 tacccccggg tggattcccc cgaacacgcg ggtgagatcg cgtgaaaacg cggccgcggc
      661 gcggagtgtc taggttgtcg atgcatgggc aaaaccgggt ctttccattg atgccacaac
      721 gaactgcagc cgcgggccac atcggccgaa aacgcgtggc acgcgccttg cccttctggc
      781 cgctcggtag gcgtcgtcga ccagcgtggt cggcgaactc gccgggactc gtgagggaga
      841 gtcgaccatg gaatcgtcgc ggcaattgtt cgtggacatg aaggacgata cgaggcgcct
      901 tcgcccctcc tacgtcttca tgatgcgatg cctgctctat tcgaggctgg aaaccggcaa
      961 cgagaccggc agcgacgtcc cggacgagga cgtcaacgtc tacctggccc acctgctcca
     1021 gtcgttcagc gaccccgact acgtggaggg ggcccgcccc tacctgcacc ggtacgacca
     1081 cgaggtgttc aagcgcctcg aacggtcgac cgacgcccga ctcaagtatg tcatctacaa
     1141 gacgaacgcc gacttcctgc tggtctcgat cggcgttttc gacaatcccg gccagtggct
     1201 ggcccatcgc ctcccccagg tccagccgtc gaagccaagg accggggagc cgtcggaaga
     1261 agcgtcgatg gggcgcggca agacgtatta ccacttcgcc tggtcctaca gccagcaggt
     1321 caatcgcgga cacccgggga tcacggaagt cctggaaaaa ctgtcggtgg ggttcgaccg
     1381 ctacatccgg gtcctctcgc acatgcgggg ggagtacctg gatctcatga aggcgctgtc
     1441 gaccggcgaa atctatcacc tcgagcgaaa catcaacaag gaacaggagc gccagcagct
     1501 caaggagagc caggacgcct tcctcgacag ctggctggcc tggaagcgct ccggcgcgcc
     1561 cgagacgctc gaggagctcc gccggcacgc ggccgccatc cgggccgtcg atccggaatt
     1621 ccaattcagc gaagacgaca ccgcaccgga aatcccgaag ctggcgccgg aaccggttcg
     1681 gccggccagg agcccggcgc gccagcgaaa accgatttag gaaccctcac cccggcgttg
     1741 ccgggacagg gttccgaaac ctcctcccaa ggtccctcgc cttgcaccat tgagggagga
     1801 ggtccttctt agccgccggg tcgtcgttcg tcaccgacac cgccctgttg gaacgatcgg
     1861 cggcccgacg gcagctttcc accgacgggc ctgctcgatg ggcccgaacg acgggtccca
     1921 cgaacatgcc gagggtcggc acccctcccg ccgcgcttgt cgcggtccgc tccggggagg
     1981 ccgaccctcg caccatgtca ccgtcaactt gccggtgtcc ctgttaccgg cacctgccgc
     2041 cgcgccgatc atcgcgcacg gtcagcgcgg cttgcgatcc accaggatca gcaatgcccc
     2101 gaccacgatg gccgctccac ccgccagggg tgggaacgaa acccgctctt tcttggtcac
     2161 cgaagcctcc agctcaccga tgtcgagcac cttctccttc ttgttgtacg tgatgccacc
     2221 gtagagaagg ccgatgatgc cgaggccgat caggatcaat ccgaaagtct tcacggtcgg
     2281 gatactcctt tcgagtcagc ggccggattc gaacgggccg atcggggccc ttcgtcagac
     2341 cgggcgacgc ccggaaatga cccgcagaag gatgctcacg actgcaagaa cgagcaggat
     2401 gtgaatgaag ccgcccatcg tgtatgacgt gacgagcccc aggagccaca gaagcagcag
     2461 gacaacagcg atcgtgtaca acatggacgt aacctccctc acagtcggtt gactggattg
     2521 actgactgac cttctcttca ccgttcagaa gtcactgaac gggatccagt caatgcaacc
     2581 ggggggccaa ttggcacgcg aggccggctg aggggcgcag aacggctccg catcggcact
     2641 tagcgcaggt gggggcacgg cggcgggaac ggttcgttac cgggcgcgcc ccgattgtcc
     2701 gccggtacat tctcccggca cggaaaaatc tacgcggact tcgagtgagg accggcgtcg
     2761 cggatggcca gcgtgcagaa gaaggcgccc aggtaggcgg ccattcccac ccagagcgac
     2821 gcggtgaaac ccgacgccag tgccacgatc acccccgaca ccgagccgag cacgctggtg
     2881 gccccgttca cgccccagca ccaggggatg gtgcccgggc tccaggccga tacgcgccgc
     2941 atcatcaccg ggaacggcat ccccatgagg aggccgagcg gcaggaggag cagcatcgac
     3001 acggccacct tcaccccctg cgactgattc tgcgtggcgg cgaacacctt ctcgaggccc
     3061 aggttgtaga ccaccgtgag cacgagcagg gccgggatcg aacgcttgag ccagccgagc
     3121 gacgtaccgg gcgccatgaa gcgcgtgagg tacgagccga cgccgctgaa cagcaggatc
     3181 gagaagagga tggtggtgat ggcgtagatc gggtgtccca ggtacagcac gaaacgctgc
     3241 atcagcggca cctcgatcat gatgaagccg aggccgatca tcgtgaagaa taggagagtg
     3301 gggagcgtcc ccggcttctg ctcgaaccgc gcgcgccggg ccaggaagag cggcaccagg
     3361 atgaagagca ccgtgagcac ggtcacgatg aagaagaggt tcaggaggat ctgcgccggc
     3421 accttcccgt acttgatgtc gtagttgccg gcccgcgtct ggttgagccg cgagaacggc
     3481 accaggttga aaaagaacgg cttgtcgtcg gtcggcgggt aaaggttgag cggctgggcg
     3541 ttgacccatt cgttgaagtc gggcgtggtg atcagctcgt ggaactgcgg gtggttgctc
     3601 acgcggggcg agtggagcac cttccaaccc atctcgcgcg ccttcccgtc gaggatgtcg
     3661 agctcggcgt cggtgaaagc cgacttcttc atcaccatcg cgccgtaccc ttcgttctgc
     3721 ttggtgatga cgatgatgtg ccgcgccgcg tcggccacgc cgttggccga tagcgtggcc
     3781 cgggccagcc ccgtcatccg gagcatcagg cccggatagt ccgaggtcat cacctggctg
     3841 gccatcatca ttccgtccgg cgtcaggtgc ttccagtatt cctggaacgc ctcccgcgtg
     3901 aagagattgt tctcggccat catgaaggcg ccggaggtgg tggccgtgaa cgtggtcgag
     3961 gagacgaagg cgatgacgtc gtacgtctcg gtggagcggg cggtcacggt gcggccgtcc
     4021 cccaccacca cgcgcacttc ggggcgcgtg tagatgtgcc cggtgatgtc gtcgaacttc
     4081 gtctttgtcc actccaccac ggtcgggttg atctcgacgc aggtaacatc cttcgagccg
     4141 agcccgatgg ccgccagcgc attctgcccg ccgccggagc cgatgtcgag gagcttgcct
     4201 cccggcttca ggaagagcgg caggaccgac acgtccgtgt agatccagtc gaccgccgac
     4261 caatcccccg aaaagggcac cgcccaggtc atggcgctcc ggtcgagctg gaagaggatc
     4321 tggtcgtcgg ggaagaaggt gctgtcgggt gagtgccccg gccggaagac gggacggttg
     4381 gaaaggttga aggccagcac ccgcgccatc gggttccact tggccgccag cggctccacc
     4441 cactcctggc tgtcaggccg ctggatggcc cgcgagccga cgttcaccgg cttgaacggc
     4501 gtggccacgt tcacgaccag cagcgcaccg atggccgccg cggtgaacca cgccaatcgt
     4561 cgttccgaag gccccgccag ttgcgcgaac gccacggccg agagcgcccc gatcacccca
     4621 accaggatga cgatgtcggg ccccgtcagg aacgtgaggc cggggaacac gagtgcgcac
     4681 ccgatggaag cgcccaccag gtcggcgaag tagagcgtgg acacgccacc cgaccagtgc
     4741 gtcagcgcca gcgaaatggc ggtgcccccc aggaagaatg ggatggcaat gagcacgtag
     4801 gtggcggcga gatacagcaa gccccagcgc ggactgtccg cggcggcggg aaggctcctt
     4861 tgaagaatga aacagagcgg caccgcgatc gcgaacagga gcgccgagcg cgccatctgc
     4921 tcgggcgccc gctcacggct gaaccgcttc gggttcaggt acacccagat gcccgatacg
     4981 ccgatgccga acagcgtgat cgacaccacc atgaaggcaa agtggtagaa catcgtcacc
     5041 gagaagatgc gggtgaggat gatctcctgc agcagcatcc ccatcgagat gaagaagatg
     5101 ccgaagagaa gggggcgcgg aagcggcgcg ttcgtggcgt tcatcgggga gtcgggaggg
     5161 tcatcggctg gttatcggcg ggccgggcgg aaaccccagc gccgaaacag ttaccgaacc
     5221 tcgtaattgg ggggttcctt ggtgatggcc acgtcgtgcg gatggctttc gcgcagcccg
     5281 gccgccgtca ccttgatgaa ccggctcttc gtggccaggt cgggcacatt gtccacgccc
     5341 acgtagccca tgccggcccg gagccccccc accatctgga acaccaggtg cgtgagcggc
     5401 cccttgtagg gcacccgtcc ctcgattccc tcggaaacga gcttgcgccg atcggtgacg
     5461 cccccctgga aatagcggtc ggccgaccct tcctgcatgg cggccatcga gcccatcccc
     5521 cggaagactt tgtacgagcg cccctcgagc aggatgatct cccccgggct ctcctcggtg
     5581 ccggcgaaca gggcgccgat catgacggtg gaggcacccg aggcgagcgc cttcacgata
     5641 tcgcccgagt agcggatgcc gccgtcggcg atgaccggca cacccgaagg cgcagccgcc
     5701 gccacgcact ccatgatggc cgtgatctgc ggaacgccga tgccggccac cacgcgggtg
     5761 gtgcacgagg cgccgggccc catccccact ttgatggcgt cgacccccag gccgatcagg
     5821 tcttcggccg cccgcttcgt ggccacgttc cccacgatga gctgcacgcc gggatgagcg
     5881 gtccggatct gcttcaccag cgacaacacg ccttcggaat gcccgtgcgc cgaatcgagc
     5941 acgatggcat cggcatgctg cgaggtgaga tgcgccacgc gctccaccgc gtcgcccgcc
     6001 acgccgatgg ccgccgccac ccgcaggcgc ccgtggctgt ccttggtggc attcgggtac
     6061 tggagcttct tctggatgtc cttgacggtg atgagcccct tcaggacgtt cttctc
//
LOCUS       DASVOD010000573         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_819946, whole genome shotgun sequence.
ACCESSION   DASVOD010000573 DASVOD010000000
VERSION     DASVOD010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819946"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1401
                     /locus_tag="VF720_12655"
     CDS             211..1401
                     /locus_tag="VF720_12655"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HEX7880256.1"
                     /translation="MSPSPRSPFDAPARFLVTAPAGLSGLLRDELAELGCEAVLTPAG
                     VAVVEGGWDVAARILIRSRIGSRLLLSVREFSARTSEMLYNQVRRVDWRSLFPPTMSI
                     AINGYGTADGTDYKLSFAPLKIKDAVCDEFRKLGGPRPDVDRMNPDVRMAAFFFNGRC
                     ELSIDLCGRPMHRRGYRAEGAAAPIRENRAAALLRFAGYDGAAPLIDPFCGSGTIPIE
                     AAWIARRMAPGLARPVESWAGARLIPALAEALEAERGAAMAEVLPEAAHPIVGRDIDA
                     TTLATARSNARLAGVDGNIRFESGDARDLAATGGWIVANPPYGERLAEGDGDAAALIS
                     DFVRRVKHHGTGATLGLVLPRGEMAAAVGLKPKRKLPLESGPLGLSFLIFEIYAGSRR
                     PARA"
     gene            1398..>1857
                     /locus_tag="VF720_12660"
     CDS             1398..>1857
                     /locus_tag="VF720_12660"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX7880257.1"
                     /translation="MNPSSIREVADDAVIVSFGDAVTPGATERIRRLATVIASAPPNW
                     IRNWRPAYTTVLVDFDPAMVDVDDVRRYLEGACASDNTPGSAPGRVVEVPVCYEAPYA
                     PDMADVAAHLGLAPDDVVARHTAGRYPVLFLGFCPGFAYLDGLEPALATPR"
BASE COUNT          267 a          694 c          605 g          291 t
ORIGIN      
        1 tccgcaaccg gccaggacgg acaggcaggc ggccacccag gccgccctca agatggatct
       61 catcatcaca ccctcctcat cgttgcgccg ggggtgaccc cggcagcggc ctcacaacct
      121 gggatgcctg gccgtgagac gggaggacgg ccgaccggtt acaggtcatt tcaaacggca
      181 cgcgagtata ctgccggccg gaggaaattc gtgtccccat cgccccgttc accgttcgat
      241 gctcccgccc gcttcctcgt caccgcaccc gccggcctgt ccggcctcct gcgcgacgaa
      301 ctggccgaac tcggctgcga ggctgtcctg acccccgccg gcgtggcggt ggtggaagga
      361 ggctgggacg tggccgccag gatcctcatc cgctcgcgga tcggctctcg cctgctcctc
      421 tcggtccgcg agttctcggc acgaacctcc gagatgctgt acaaccaggt acgacgggtc
      481 gactggcgat cgctctttcc acccacgatg tcgatcgcga tcaacggcta cggcaccgcc
      541 gacggcaccg actacaagct gtcgttcgcg ccgctcaaga tcaaggacgc ggtgtgcgac
      601 gaattccgca agctcggcgg gccgcgcccc gacgtcgatc gcatgaaccc cgacgtgcgc
      661 atggccgcct tcttcttcaa cgggcgatgc gaactctcca tcgacctgtg cggccggccg
      721 atgcatcgcc gcggctaccg cgccgagggc gccgccgccc cgattcgcga gaaccgggcc
      781 gccgcactcc ttcgctttgc cggctacgac ggcgcggccc ccctcatcga tccgttctgc
      841 ggcagcggca cgatcccgat cgaggcggcc tggatcgcgc ggcggatggc gcccggcctc
      901 gcgcgacccg tggagagttg ggccggcgcc aggctgatcc cggcgctggc cgaggcgctc
      961 gaagccgagc gcggcgcggc catggccgag gtgcttcccg aggccgcgca cccgatcgtc
     1021 ggtcgcgaca tcgacgccac cacgctggcc accgcccgct cgaacgcccg tctggccggc
     1081 gtggacggca acatccgttt cgagtcgggc gatgcccgcg acctggcggc caccggcggc
     1141 tggatcgtgg ccaatccccc ctacggcgag cggttggcgg agggtgacgg cgacgcggcc
     1201 gctctcatca gcgacttcgt ccgccgggtc aagcatcacg gcaccggcgc cacgctgggc
     1261 ctcgtgctgc cgcgcggcga gatggccgcg gcggtggggc tcaagccgaa gcgcaagctg
     1321 ccgctggaga gcggtccgct cggactttcg ttcctcatct tcgagatcta cgccgggagc
     1381 cgccgccccg ccagggcatg aacccatcct cgattcgcga agtggccgac gacgccgtca
     1441 tcgtgtcgtt cggagatgcg gtcacacccg gggcgaccga gcgcatccgt cgactcgcca
     1501 cggtaatcgc ttcagcaccg ccgaactgga ttcgcaactg gcggcccgct tacacgacgg
     1561 tgctcgtcga tttcgatccg gcgatggtcg atgtcgatga cgtacgtcgg tacctggaag
     1621 gtgcctgcgc ttcggacaac accccgggtt ccgcgccggg ccgcgtcgtg gaagtccctg
     1681 tctgctacga agcgccctac gcgcccgaca tggccgacgt cgcggcgcac ctcggtctcg
     1741 cgccggacga cgtggtggcc cgccatacgg cgggccgcta tccggtcctt tttctcggat
     1801 tctgccccgg cttcgcctac ctggacggcc tcgagcccgc gctggccaca cctcgcc
//
LOCUS       DASVOD010000574        12890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_820014, whole genome shotgun sequence.
ACCESSION   DASVOD010000574 DASVOD010000000
VERSION     DASVOD010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 12890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12890
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820014"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF720_12665"
     CDS             <1..816
                     /locus_tag="VF720_12665"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX7880258.1"
                     /translation="DHPRPSELVPTTEALLEDLRGFCVTKGIVTIPGNERCKAMATPE
                     FMRDFIFAAMDTPGPFETKGREAWYFVTPPDATKPALQQDDDMRFFNRYSLPILSMHD
                     SWPGHYLQFLKGEKTTSKVRKVFGSTSFSEGWGLYCEQMMLDQGWGGNDPKYKLFQLR
                     LALLRACRYLVAIRMHTGDMTMEQAADFFVKEGFTERVDAEREARRGTLDPMFLSYQL
                     GKLQILKAREDYKRFKGAAFNLKDFHDRVLSEGEPPVKLLRRLLMPGDKGTLL"
     gene            835..2433
                     /locus_tag="VF720_12670"
     CDS             835..2433
                     /locus_tag="VF720_12670"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX7880259.1"
                     /translation="MTDATDFLLRHVAEAAPLAADYHEKYWATALSGRGEDAARSAAA
                     KARLLELYSRPAEFAEVRRLRAAGGADPLTDRQLTLLDLEYSGRQVDPAVLADIAARE
                     EAIEQSFNSFRALLDGESVNENTLRRILKEESDVERRRTAWEGAKQVGRAVAPAVLEL
                     IERRNVEACRLGYRDHYAMALAHQELDESTLFTLLGRLKELTEAPFRRIKADIDRTLA
                     ARYRLSDWNSRPWLYADPFFQESPPGAAGVDLDAVFADQDVEALTVRTFEGVGLPIRA
                     LLPRSDFVEREGKNQHAFCLHVDREGDVRVLCNVRPSEYWMSTMLHEFGHAVYDAGLD
                     FDLPWLLRSPAHTMTTEAVAMLFGRLTRNAEWLTRVAGVPATEAARLAAEVKQSLVIG
                     QVIFARWGLLMVHFEREMYRDPRQDLNRLWWRMARDFQGVEAPPDRNEPDWASKIHLA
                     CAPVYYQNYLLGEMTASQLESAARDRLNSSGTATLVDSKEAGRFLSEQLFRQGARRDW
                     NDTMRHATGAALDPEHWIRQFVAG"
     gene            2489..3796
                     /locus_tag="VF720_12675"
     CDS             2489..3796
                     /locus_tag="VF720_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX7880260.1"
                     /translation="MKKNYVLDTNVLLHDPGALLSFEDNTVVVPIHVIEEIDTFKKQA
                     SELGRNAREVSRRLDQYQQLGQLSRGVSLENGGHLRVILDPSGNGNGDYGSRSVDDAL
                     LRIAKSLQSAEPGVPVILVTKDTNLRIKADALDLSAEDFEADQVRLSEMSSRQRELKV
                     EPGVIDEFQKAGSIALPPGDFEPNAYLNLLDGNGTGRSSLGRVNLRDGVIQRLKPHRD
                     EVFGLKPRNREQYFALDALLDDEIKLVTLMGKAGTGKTLLALAAGLHKTRNEGTYLKM
                     LVSRPIFPLGRDVGYLPGRIEEKLDPWMQPIYDNLEFLLESQQGKKHPPSIEKLMTTG
                     ILGIEPLTYIRGRSMPNQFFLVDEAQNLTPLEVKTIVSRVGEGTKIVLTGDPTQIDNP
                     YVDAASNGFNHLVQRFRGVPLAAHVELIKGERSALAELASDIL"
     gene            complement(5067..5810)
                     /locus_tag="VF720_12680"
     CDS             complement(5067..5810)
                     /locus_tag="VF720_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880261.1"
                     /translation="MTQELLLSAVLAIWLIPFLFGGHFRLGATHGRRSLLSAAAGIAV
                     GYVFIDLLPEMARLQEEFTTAAAGLGRPFFRYRVYSSAMIGFVFFFVLENMVVSARAE
                     ARSPEAGENAMYWAHLMGFTAYGGLMSYLLWAEEEFDPAGLALYSLAMFLHFWTVDHS
                     LREEHGARYDRYGRWVVVAGLVAGALVGGRGVTSELWVPTIMGFIGGGVVINSLKDEL
                     PGRGEGRAMPFVLGAFGYSLLLLVLGTRH"
     gene            complement(5807..7471)
                     /locus_tag="VF720_12685"
     CDS             complement(5807..7471)
                     /locus_tag="VF720_12685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX7880262.1"
                     /translation="MPFRRIAPGAAMLGRYQAGWLGADIAAGLSVAAVAIPTAIAYAQ
                     LAGFPAEVGLYSAILPLVAYALFGSSRQLMVNPDAATCAMVGAIVAPMAAGDINLQVA
                     LASALAIFSGVLCVVAGALRLGFLADFLGRPVLVGFMNGIAISIFLGQIGKLFGFPLE
                     SNGIFPRLMEFVTRLPSTHVPTLAVGVATFATLRLVRRFLPRWPSPLVALVMAVLLVR
                     LFHLDERGVAVLGSLPAGLPPIRWPEFPLELLKPLLESAIGLALVSVTSGMVTARSFA
                     ARNREELDVDREFIAFGAGNVASGLSGGFAVTGADSRTAVAASMGGKSQVTGLVAALA
                     LALVLLFLTGPLQYFPICALGAILISAALGLFDMASLRRLARIKAGEFAVSVVAMLGV
                     VVLGALEGIAVAVVLALMVLLLRSSRPVDAVLGRIAGLDGFHSVARYPEARQVPGILV
                     YRFNSSLIFYNCAYFRRRILEHLAAHPDTRQVVIDGSPIVHVDSTGADMLSQLADELR
                     ANDIRLVLAEMHVRVLGILERSEALAHLGPGANFPTLEAAIEAAKA"
     gene            complement(7475..8275)
                     /locus_tag="VF720_12690"
     CDS             complement(7475..8275)
                     /locus_tag="VF720_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX7880263.1"
                     /translation="MNNNGLPLGIVPIALYAVGTFLGMLLMLELGRLVGRRGLAKHGG
                     QSHSTSPVDGAIFALLGLLIAFTFSGAAARFDTRRLQIATEANHIGTSWLRLDLLPAD
                     YRHELRGLFRQYLDARLDTYRKLPDVNAALAELARSQELQRQIWSKAVAGCDARADPS
                     VRTLLLGSINEMIDITTTRTMAARIHPPTVIFLLLTCMTLGCSFLAGHAMAGHPGRPW
                     VHHLVFAIVISSVTYTILDIEHPRAGVIRISAADQVLEELRHEMDLPD"
     gene            complement(8303..8989)
                     /locus_tag="VF720_12695"
     CDS             complement(8303..8989)
                     /locus_tag="VF720_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7880264.1"
                     /translation="MFIGKRNAAGPGTKTVRTALLALAFPLLLPVVASASQGVDVFVF
                     GGYTTKSELDGAGTLLGGQLTVDGAATAGISLGTTTPQNIGIEAMWTHQWSGLTFDPV
                     GGQEMSVSDMSIEQFHANFLFYPPGHINAPTKPYFLIGLGANIFNPSREAADSETRFT
                     WALGLGLKRAVNEKVGFRAQLRYTPTYIGSNDEGYWCDPYYGCYTVIDPNYLDQWDFT
                     GGLVFNLGRS"
     gene            complement(9217..9696)
                     /locus_tag="VF720_12700"
     CDS             complement(9217..9696)
                     /locus_tag="VF720_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880265.1"
                     /translation="MKRTKLSILALLAVTTLTLPALLLAQKESEIQSVRADLQADRQA
                     VVAANLPMTEAESAAFWPVYREYRAEMAVVGDGLQKLITDYAATYNKSAMTDEQGMMM
                     TKEYLKLRKESLSIREKYVGKFGKIIPGKSVMRLYQVENKLDVVVDASLAGNIPLAE"
     gene            9805..10182
                     /locus_tag="VF720_12705"
     CDS             9805..10182
                     /locus_tag="VF720_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880266.1"
                     /translation="MDDLLTRLAENMHIRATGPMKFRLILQPLMASIFAIMAGLKDAR
                     AGRSPFFWGLINDPANRADMLKDGWKSVGKVFVIALVLETVFQVIVLKTVYPLEALSM
                     ALLLAIVPYLLLRGLTSRLASRR"
     gene            complement(10202..11704)
                     /gene="pepD"
                     /locus_tag="VF720_12710"
     CDS             complement(10202..11704)
                     /gene="pepD"
                     /locus_tag="VF720_12710"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HEX7880267.1"
                     /translation="MATTPTSSTTAFAGLEPARLWELFAELTTIPRPSKNEAAVLAWV
                     DAWAASRGFAIRADGAGNRVVRIPATPGREGAPVVALQAHVDMVSEKHSWSTWDPDDG
                     RILIVKDGDWLVAPETTLGADNGMGVTLMMFLAESPGMPHGPLELLFTTDEETGLTGA
                     RDLDPALVTARTLLNLDSEEDGVLYIGCAGGTDTTLTWSGKPVVVPPGWRGVSLEVSG
                     LTGGHSGGDIHLDRANSNKLLARVLREIAARTPLRVSAISGGNKRNAIPREARVTLAL
                     PPVPAGGSDPVAEAVALVIASARAQYADREPTLAVVAMPAEVPPSAHDEAASASLVTL
                     LSALPSGVVAMSQAVPGLVETSTNLAVIRTAPAAEGAGITITSSSRSSDRHAMEEVAE
                     SLAAIGRLAGCRITSFDKYGGWKPAPASNVVRVAAEAHRRLFGTEPPITAIHAGLECG
                     LFAEKFPGIDMVSFGPDIRGPHAPGERVSISSTARFVRWLAGVLDDLSRL"
     gene            complement(11705..12844)
                     /locus_tag="VF720_12715"
     CDS             complement(11705..12844)
                     /locus_tag="VF720_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX7880268.1"
                     /translation="MGFSTDAIHIGQEPDPTTGAIIPPIYQTSTYVQQGIGDHKGYEY
                     ARTHNKTRDHLERCIAALEKADHGFAFASGMAATTTLLHLVKAGDHVLAGNNMYGGTY
                     RVMSLVFADHGLNFSYVDMSDLGAVEAAIRPETKMIWFETPTNPMMGLADLSAIAAIA
                     RKRGILTVCDNTFMSPYFQRPIEHGIDIVYHSTTKFINGHSDMVGGLVLTSHKASAER
                     LTFLRNAMGTVPGPFDCWLALRGVKTLAVRMDRHDANGRRIAGWLKEQSKVKRVIYPG
                     LPEHPQHELARRQMSGFGGMLAFDAGSIENANKVLKAVKIFSLGESLGGVESLISHPA
                     TMTHASVPKADREALGLTDGLVRLSVGIEDVEDLVADLETGLKAL"
BASE COUNT         2316 a         4293 c         4287 g         1994 t
ORIGIN      
        1 gaccaccctc gcccgtccga gctggtgccg accaccgagg cgctcctcga ggacctgcgc
       61 ggcttctgcg tcacgaaggg gatcgtcacg atccccggaa acgagcgatg caaggcgatg
      121 gccaccccgg agttcatgcg cgacttcatt ttcgcggcca tggacacgcc cgggccgttc
      181 gaaacgaagg ggcgggaggc gtggtacttc gtcacgccgc cggatgcaac gaagccggcg
      241 ctgcagcagg acgacgacat gcgcttcttc aaccggtatt cgctgccgat cctctcgatg
      301 cacgattcct ggcccgggca ctacctccag ttcctcaagg gggagaagac cacctccaag
      361 gtgcgcaagg tgttcgggtc gacgtcgttc agcgaaggat ggggcctgta ctgcgagcag
      421 atgatgctcg accaggggtg gggcggaaac gacccgaaat acaagctgtt ccagctgcgc
      481 ctggcgctgc tccgggcctg ccgctacctg gtggccatcc gcatgcacac gggtgacatg
      541 accatggagc aggcggccga cttcttcgtg aaggagggat tcaccgaacg cgtggatgcc
      601 gagcgcgagg cgcgccgggg aacgctcgat ccgatgttcc tctcctacca gctcggcaag
      661 ctgcagatcc tgaaagcgcg cgaagactac aagcgcttca agggggctgc gttcaacctc
      721 aaggatttcc acgatcgggt gctctcggag ggtgagcccc cggtgaagct cctgcgccgc
      781 ctcctcatgc ccggggacaa gggcaccctg ctgtgatacc gtcggcaacc gacgatgacc
      841 gacgccactg acttcctcct ccgccacgtg gccgaggccg ccccgctggc ggcggattac
      901 cacgagaagt actgggccac tgccctgtcg ggccggggcg aggacgcggc acgctcggcg
      961 gccgccaagg ccaggctcct cgagctttac tcacgccccg ccgaattcgc cgaagtgcgt
     1021 cgcctccgcg cggcgggcgg cgccgatccc ctgaccgatc gccagctcac cctgctcgac
     1081 ctcgagtatt ccgggcgcca ggtcgacccg gccgtcctgg ccgacattgc cgcgcgcgaa
     1141 gaggcgatcg agcagtcgtt caattcgttc cgcgccctcc tggacggtga atcggtcaat
     1201 gaaaacaccc tgcgccggat cctcaaggag gaatccgacg tcgaacggcg acgcacggcc
     1261 tgggaaggcg ccaagcaggt cggccgggcc gtggcgccgg ccgtcctcga gctgatcgag
     1321 cggcgcaacg tggaagcgtg ccgcctcggc taccgcgacc actacgcgat ggcgctggcg
     1381 caccaggagc tcgacgaaag cacgctcttc acgctgctcg gccgcctcaa ggagctgacc
     1441 gaggcgccgt tccggcgcat caaggccgac atcgaccgga ctcttgccgc ccgctaccgc
     1501 ctgtccgact ggaacagtcg accgtggctc tatgccgacc ccttcttcca ggaatcgcct
     1561 cccggcgcgg ccggcgtcga cctcgacgcc gtcttcgccg atcaggatgt cgaggcgctt
     1621 accgtccgca cctttgaggg ggtgggcctc cccatccggg cgctccttcc ccgttccgac
     1681 ttcgtggaac gcgagggaaa gaaccagcat gccttctgcc tgcacgtcga ccgggaagga
     1741 gacgtgcgcg tcctctgcaa cgtgcgcccc agcgagtact ggatgtccac gatgctccac
     1801 gagttcggcc acgcggtgta cgacgcgggg ctcgacttcg acctgccgtg gctgctccga
     1861 tccccggccc acacgatgac aaccgaggcc gtggccatgc tcttcggccg gttgacacgc
     1921 aacgcggagt ggctcacccg cgtagccggc gtgcccgcga ccgaagccgc ccgcctcgcc
     1981 gccgaagtga aacaatcgct cgtcatcggg caggtgatct tcgcccgctg ggggctcctc
     2041 atggtgcatt tcgaacggga gatgtaccgc gatccccgcc aggacctgaa ccgcctctgg
     2101 tggcgcatgg cccgggactt ccagggagtc gaggcgcccc ccgaccggaa cgagcccgac
     2161 tgggccagca agatccacct ggcctgcgcg ccggtgtatt accagaacta ccttctcggc
     2221 gagatgaccg cgagccagct cgaatcagcc gcgcgggacc gcctcaacag ttcgggtacc
     2281 gccacgctgg tcgactcgaa agaggcgggg cgattccttt cggagcagct gttccggcag
     2341 ggagcccgcc gcgactggaa cgacacgatg cgccatgcca cgggggccgc cctcgatccg
     2401 gaacactgga tccggcagtt cgtggcgggc tgaccttttc cgtacaactc cgggggccgg
     2461 ccatgtccgg tgcccgaggg ggctccgcgt gaagaagaac tacgtgctcg acaccaacgt
     2521 cctcctgcac gatccggggg cgctcctctc gttcgaggac aacaccgtcg tcgtcccgat
     2581 ccacgtgatc gaggaaatcg acaccttcaa gaagcaggcc tccgaactgg ggcgcaacgc
     2641 gcgcgaagtg agccgccggc tcgaccagta ccagcagctg gggcagctgt cgcgcggcgt
     2701 ctcgctggag aacggcggcc acctccgcgt gatcctcgat ccgagcggca acggcaacgg
     2761 cgattacgga tcccgcagcg tggatgacgc gctccttcgg atcgccaaga gcctgcagtc
     2821 cgccgagccg ggcgtgccgg tgatcctggt caccaaggac acgaacctcc gcatcaaggc
     2881 cgacgcgctc gacctttcgg ccgaggactt cgaggccgac caggtgcggc tgtcggagat
     2941 gtcgagtcgc cagcgtgagc tcaaggtgga gcccggggtg atcgacgagt tccagaaggc
     3001 ggggagcatc gccctgccgc cgggggactt cgagccgaac gcctatctca acctgctcga
     3061 cgggaacggc accggccgtt cgtcactggg acgggtgaat ctccgggacg gcgtgatcca
     3121 gcgtctcaag ccccaccggg acgaagtgtt cggattgaaa ccacgcaacc gcgagcagta
     3181 cttcgcgctc gacgccctgc tcgacgacga gatcaagctc gtcacgctca tgggaaaggc
     3241 cggcaccggc aagacgctgc tcgcgctcgc ggccggcctc cacaagacgc gcaacgaggg
     3301 gacgtatctc aagatgctgg tcagccgccc catcttcccg ctcggccgcg acgtcgggta
     3361 tctccccggc cgaatcgagg agaagctcga cccgtggatg cagccgatct acgacaacct
     3421 cgagttcctg ctcgagagcc agcaggggaa gaaacatccg ccgtcgatcg agaagctgat
     3481 gaccaccggc atcctcggca tcgagccgct cacctacatc cgcgggcgtt cgatgcccaa
     3541 ccagttcttc ctggtggatg aagcgcagaa cctgacaccg ctcgaagtga agacgatcgt
     3601 gagccgcgtg ggcgagggaa cgaagatcgt cctcaccggc gatcccaccc aaatcgacaa
     3661 tccgtatgtc gacgcggcat cgaacggctt caatcacctc gtgcagcgct tccgcggcgt
     3721 gcctctcgcc gcgcatgtcg agctgatcaa gggagagcgc agcgcgctgg cggaactggc
     3781 gagcgatata ttgtagcgac ggctacgccg gcgctacgga cagcccctct ccccgccgaa
     3841 atctctccac atttgtcccc acgccccccg cggcactctc cacacatctc aacatgcttt
     3901 ttccacgttt cacttctcga accccgcaac catttcgcgc cctcacgctt tccgacttct
     3961 ccacaaatgt ggaaggcgca cggtttgcgt gatcgacgca tcgatgctcg cgcgcgaggc
     4021 acacttttct tttcttttgc gcacatttcg cttgcgtgta cgttgcgcga agcgcaacgt
     4081 acgacctgga gacggaaacg agcccgctcc ggcgccgaac ggcgtcgccg ccgaaaggag
     4141 gcgatgcgcg aggcgcgaag acctcaggag ggtcggttca ctcttcggag tgagcctggt
     4201 ccggaggcga tcgaaacttc gaggggcacg acggaacgac caccccggtg gttgatccag
     4261 cgaaagcccg cggaaggaga cggccggaag cggtcgtcgt cgcgccgggt gaggagccct
     4321 ggaggggatg aaacccaggg gaggatcggg tcttccatgg cggttaatcc cgccgcggtc
     4381 gactacggac ttccgggggg atgaacaccg ggcagccggg caaggcctcg cgggatcaaa
     4441 acctcgagcg ccattcgagc caacggcacg ccggtagagg gtcggctgta aggccgcccg
     4501 atccccggaa gggaaagcct ccgaagggca gaaccccacg agcgccatcg gcatgcaaca
     4561 aggccgaggg gcgcggagag gatcaaaccg ccaggagggt gcgaaaccct ggaggcggca
     4621 ggatcgggtg aggcaaaccc ggtcgtcgat tctccgcgcc gcgagcgctg aaggggaagg
     4681 aaccccatgg gagttgttcg atgccgcagg gtgtcgaacg accgtgtcgg tcacactctg
     4741 aaggggagct caagtccagg agaggctgaa accggcgttt tcacgaatgt cggcggcagc
     4801 ggaggcacga aacaccgcaa gggcccggcc gaaaggcaaa aggtcgaagc gggatcgccc
     4861 aaccagcagg gcgatacgtc ctccgtgtta atcctctgta ggaatgggca accccacgag
     4921 aggaggccgg tccgcggtga cgcggccccg gcgcccgaca tgtgtccggt actccgaagg
     4981 gggcacggag ggctcatccg tctccacttt tttatgccca ccgccgcgca cgcgcgccca
     5041 ctccaacacc gccgcgcgcc cgcccctcag tgccgcgttc cgagcaccag gagcaacagg
     5101 gagtagccga acgctccgag cacgaagggc atggcccgcc cttccccgcg ccccggcagt
     5161 tcatccttca agctgttgat gaccacgcct ccgccgatga atcccatgat cgtcggaacc
     5221 cagagttccg aggtcactcc ccgcccaccc accaatgcgc ccgccaccag gccggccacc
     5281 accacccagc gaccgtagcg gtcatatcgc gcgccgtgct cctcccggag cgaatggtcc
     5341 accgtccaga agtgaagaaa catcgcgagg ctgtagaggg cgaggccggc cgggtcgaac
     5401 tcttcttccg cccaaaggag gtagctcatg aggcccccgt acgccgtgaa acccatgaga
     5461 tgcgcccagt acatggcgtt ctcccccgcc tcgggcgagc gcgcctcggc gcgggccgac
     5521 accaccatgt tttcgaggac gaagaagaag acgaagccga tcatcgccga cgaatagacg
     5581 cggtaccgga aaaaggggcg ccccaggcca gccgcggcgg tcgtgaactc ttcctgcagc
     5641 cgggccatct cgggaagcag gtcgatgaag acgtagccga cggcgatgcc ggccgccgcc
     5701 gacaggaggc tccggcggcc atgggtggcg cccagccgga aatgcccgcc gaagagaaac
     5761 ggaatcagcc agatggcgag aaccgccgac aggagaagtt cctgtgtcac gctttcgccg
     5821 cctcgatggc ggcctccagc gtcgggaagt ttgcgccggg gcccaggtgc gccagcgcct
     5881 cgctccgttc gaggatgccc agcacgcgga cgtgcatctc ggccaggaca agccgaatat
     5941 cattcgcgcg gagctcgtcg gccaattggg aaagcatgtc ggctccggtg ctgtcgacgt
     6001 gaacgatcgg gctcccatcg atgacaacct ggcgggtatc ggggtgggcg gccaggtgtt
     6061 cgaggatgcg ccgacggaag taggcgcaat tgtagaagat gagcgacgaa ttgaaccggt
     6121 acaccaggat gcccggcacc tgccgtgcct cgggatagcg cgccaccgaa tggaagccat
     6181 cgagcccggc aatccggccg agcaccgcgt cgaccgggcg ggaggaacgg aggagcagga
     6241 ccatcaaggc caggaccacg gccaccgcga ttccttccaa cgcgcccagg acaaccacgc
     6301 cgagcatggc caccacggag acggcgaact cccccgcctt gatgcgcgcg agccgccgga
     6361 gcgatgccat gtcgaagaga ccgagggcgg ccgagatcag gatggctccc agcgcgcaga
     6421 tgggaaagta ctggaggggc cccgtcagga agaggagaac cagcgccagg gccagcgcgg
     6481 ccacgaggcc ggtcacctgg ctcttccccc ccattgaggc ggcgacggcc gttcgcgaat
     6541 cggcgccggt caccgcgaac ccgcccgaga gaccggaagc gacgttgccg gcgccgaacg
     6601 cgatgaattc gcgatcgacg tcgagctcct cgcgattccg cgcggcgaaa ctccgggccg
     6661 tgaccatgcc gctggtcacg ctgaccaacg cgaggccgat ggccgactcg aggaggggct
     6721 tcaagagttc aagcgggaat tccggccacc ggatgggggg caaaccggcc gggagggacc
     6781 ccagcaccgc caccccccgc tcatcgagat ggaagaggcg gaccagcagc accgccatca
     6841 ccagcgcgac gagcggtgac ggccaccggg gaaggaatcg cctgacgagc cggagcgtgg
     6901 cgaacgtggc cacaccaacc gcaagcgtcg gcacatgggt cgacggcagg cgcgtcacga
     6961 actccatcag ccggggaaag atcccgttcg actcgagcgg aaaaccgaaa agcttcccga
     7021 tctgcccgag gaagatgctg atggcgatcc cgttcatgaa ccccaccagc accgggcgcc
     7081 ccaggaaatc ggccaggaag ccgaggcgca gcgcaccggc caccacgcac aggacacccg
     7141 aaaagatggc cagggccgag gcgagcgcca cctgaaggtt gatgtcaccg gcggccatcg
     7201 gggccacgat ggcccccacc atggcgcagg tggccgcgtc cgggttcacc ataagctggc
     7261 gcgagcttcc gaagagggcg taggccacga gcggcaggat agcggagtag agccccacct
     7321 ctgccggaaa gccggccagc tgcgcgtagg cgatggccgt ggggatggcc acggcggcca
     7381 ccgagaggcc ggccgcgatg tcggctccga gccagccggc ctggtaacgg ccgagcatcg
     7441 ccgccccggg cgcgatccgg cggaagggca tcggctagtc cggcagatcc atttcgtgtc
     7501 gcagctcctc gaggacctgg tcggccgcgc tgatccggat gactccggcc cggggatgct
     7561 cgatgtcgag gatcgtgtag gtcaccgacg aaatgacgat ggcgaagaca aggtgatgca
     7621 cccaggggcg cccgggatgg cccgccatcg cgtggccggc caggaaggaa cacccgagcg
     7681 tcatgcaggt gagcagcagg aagatgaccg tcggcggatg gatccgggcg gccatggtcc
     7741 gggtggtggt gatgtcgatc atctcgttga tcgaaccgag cagcaacgtg cggaccgagg
     7801 ggtcggcccg ggcatcgcaa cccgccaccg ccttcgacca gatctgccgc tgcagctcct
     7861 ggctcctggc cagttcggcc agggccgcat tgacgtccgg caacttgcgg taggtatcga
     7921 gccgggcgtc gagatactgg cggaagagcc cgcgcagctc atgccggtag tcggcgggca
     7981 ggaggtcgag gcggagccag gacgtgccga tgtggttggc ctcggtggcg atctggagtc
     8041 ggcgggtgtc gaaccgcgcg gccgctcccg agaaggtaaa ggcgatgagg agccccagaa
     8101 gggcgaagat cgcgccgtcg acggggctcg tgctgtggga ctggccgccg tgcttcgcaa
     8161 ggccgcgacg tccgaccagg cgacccagct cgagcatgag cagcatgcca agaaacgttc
     8221 cgaccgcgta gagcgcgatc ggaacgattc ccagtggaag accgttgttg ttcatgaccg
     8281 gtgtgggatc cggcgtttgg gcttagctgc gtccgaggtt gaagacgagg ccgccggtga
     8341 aatcccactg atcgaggtag ttcgggtcga tgaccgtgta acagccgtag tacggatcgc
     8401 accagtaccc ctcatcgttc gatccgatgt aggtgggcgt gtatcggagc tgggcccgga
     8461 agcccacctt ttcgttgacg gcgcgcttca agccgagccc cagcgcccag gtgaagcgcg
     8521 tttcggagtc ggccgcctcg cgggaaggat tgaagatgtt ggcgccgagg ccgatcagga
     8581 agtagggctt ggtcggggcg ttgatatggc cgggaggata gaacaggaag ttggcgtgga
     8641 actgctcgat cgacatgtcg gagacgctca tctcctggcc gccgaccgga tcgaacgtga
     8701 ggccgctcca ctggtgcgtc cacatggcct cgatcccgat gttctgcggg gtggtcgtgc
     8761 cgagagaaat gccggccgtg gccgcgccgt ccaccgtcaa ctggccgccg agcagggttc
     8821 cggcgccgtc cagctccgac ttggtcgtat aacctccgaa gacgaagaca tcgactccct
     8881 ggctggcgga cgccaccacg ggaaggagga gcgggaaggc cagggccagg agggccgtcc
     8941 gtaccgtctt ggtgccaggc cctgccgcgt ttcgcttgcc gatgaacatc cttcttcctc
     9001 cgcgtgtcgt gaagttgggg tccctggggc cgatggcccc cggacagtgt caatttttcg
     9061 gtatcctatt gagttttaat gagatgtcaa ggccccggga gcccgccgcc gggcgacatg
     9121 gaactgttta catcctccgc tccgccggcc ggatgacagg aaggccggcc gcccattccg
     9181 ggtggccggc ctccctgaat cgtgtcccgg tcggccctac tcggccagcg ggatgttccc
     9241 ggccagggaa gcgtccacca ccacgtcgag cttgttctcg acctggtaca ggcgcatgac
     9301 cgacttcccg gggatgatct tgccgaactt gcccacgtac ttctcgcgga tcgacaggct
     9361 ttccttccgg agcttcaggt actccttcgt catcatcatt ccctgctcgt cggtcatcgc
     9421 gctcttgttg taggtagccg cgtaatccgt gatcagcttc tggagcccgt ccccgacgac
     9481 cgccatctcg gcacggtact cgcggtacac cggccagaag gccgccgatt cggcttcggt
     9541 catcggcaga ttggcggcga ccaccgcctg gcgatcggcc tggaggtcgg cccggacgct
     9601 ctggatctcc gactccttct gggccaggag gagggcgggt agggtaaggg tggtgacggc
     9661 caggagggcc aggatcgaga gcttcgtgcg cttcatcgga cttcctttcg agtaagatgc
     9721 caaccggtct cggtaattcg attcgggcgc aaagagtgct gcgtcgagcg ggggctgtca
     9781 accgaaggca aggaggtgga tgtcgtggat gacctgctca cccgactcgc cgaaaacatg
     9841 cacatccggg ccaccggccc catgaaattc cgcctgatcc tccagccgct gatggcctcg
     9901 atcttcgcca tcatggcggg cctgaaggat gccagggccg gccggagccc cttcttctgg
     9961 ggcctgatca acgatccggc caaccgggcc gacatgctca aggacggctg gaagagcgtg
    10021 ggaaaggtct tcgtcattgc cctggtgctg gaaacggtgt tccaggtgat cgtgctcaag
    10081 acggtctacc cgctcgaggc gctctcgatg gccctcctcc tggccatcgt cccctacctc
    10141 ctcctccgcg gcctcacctc gcgcctcgcc tcacgacggt aacaggcacg caaggagagc
    10201 ctcacaaccg ggagaggtcg tcaagcacac ccgccagcca acgcacgaac ctggcggtcg
    10261 aggagatcga cacccgctct cccggcgcgt gcggaccgcg gatgtccgga ccgaacgaca
    10321 ccatgtcgat gccggggaat ttctcggcga agaggccgca ctcgaggccg gcgtggatgg
    10381 ccgtgatcgg cggctcggtg ccgaagagcc gccggtgggc ttcggccgcc accctcacga
    10441 cgtttgatgc gggcgccggc ttccagccgc cgtacttgtc gaacgaggtg atccggcaac
    10501 cggccaggcg cccgatggcc gccaggctct cggccacctc ctccatggca tgacggtcac
    10561 tcgatcgtga cgacgacgtg atcgtgatcc cggcgccctc ggcggcggga gccgttcgaa
    10621 tcactgccag gttggtggag gtttccacga gccccggcac cgcctggctc atggccacga
    10681 caccgctcgg cagggccgag agaagcgtga cgagcgatgc cgacgccgct tcatcgtggg
    10741 ccgagggtgg cacctcggcc ggcatggcca ccaccgccag cgtcggttcg cgatcggcgt
    10801 actgggccct ggccgaggcg atgaccagcg ccacggcttc ggcaacggga tcggatccac
    10861 ccgccggcac ggggggcagc gccagcgtga cgcgcgcctc gcgcggaatg gcgttgcgtt
    10921 tgttgccacc cgaaatggcg ctgacacgga gcggcgtgcg tgccgcgatc tcccgcagca
    10981 cgcgcgccag cagcttgttg gagttggcgc gatcgagatg gatgtcgccg ccggagtgtc
    11041 ctccggtcaa gccgctcacc tcgagcgaca cgcccctcca gccgggcggc acgacgaccg
    11101 gtttgccgct ccacgtgagc gtggtatcgg tgccgccggc gcatccgatg tagaggacgc
    11161 cgtcctcctc ggaatcgagg ttcaggagcg tgcgggccgt gacgagtgcg ggatcgaggt
    11221 cccgcgcccc ggtgagcccg gtttcctcgt cggtcgtgaa gaggagctcg aggggcccgt
    11281 gcggcattcc gggcgattcg gccaggaaca tcatgagcgt gacccccatg ccgttgtccg
    11341 cgccgagcgt ggtctcgggg gccaccagcc agtccccgtc tttcacgatc agtatccggc
    11401 cgtcgtccgg atcccacgtg ctccacgagt gcttctccga caccatgtcg acgtgagcct
    11461 ggagcgccac caccggcgca ccctcgcggc cgggcgtggc cggaatgcgc acgacgcggt
    11521 tacccgctcc atccgccctg atggcgaagc cgcgcgaggc ggcccaggca tccacccacg
    11581 ccagcaccgc cgcctcgttc ttcgacggcc gcgggatggt ggtgagttcg gcgaacagct
    11641 cccagaggcg ggcgggctcg agcccggcga acgccgtggt cgacgatgtc ggcgtggtgg
    11701 ccatctagag tgccttgagt cccgtctcga ggtcggccac caggtcctcg acgtcctcga
    11761 taccgacaga caggcgcacg aggccgtccg tcaggccgag cgcttcgcga tcggccttcg
    11821 gcaccgaggc gtgcgtcatg gtggccggat ggctgatgag gctctcgacg ccccccaggc
    11881 tctcgccgag cgagaagatc ttcaccgcct tgaggacctt gttggcattc tcgatcgagc
    11941 ccgcgtcgaa ggccagcatg ccgccgaagc cggacatctg cctcctggcc agttcgtgct
    12001 gcggatgctc cggtagcccc ggatagatga cccgtttcac cttcgactgc tccttgagcc
    12061 acccggcgat gcgacggccg ttggcatcgt ggcgatccat ccgcaccgcc agcgtcttga
    12121 caccccgcag cgccagccag cagtcgaacg ggccgggcac ggttcccatc gcgttgcgca
    12181 ggaaggtgag ccgctcggcc gacgccttgt ggctggtgag gacgagcccc cccaccatgt
    12241 cggagtgccc gttgatgaac ttggtcgtcg agtgatagac gatgtcgatg ccatgctcga
    12301 tcggccgctg gaagtaaggg ctcatgaagg tgttgtcgca cacggtcagg atgccgcgct
    12361 tcctggcgat ggcggcgatg gccgagaggt cggccaggcc catcatcgga ttggtgggtg
    12421 tctcgaacca gatcatcttc gtttcgggtc ggatggccgc ctcgaccgcg cccaggtccg
    12481 acatgtccac gtacgagaag ttgagtccgt ggtcggcgaa gaccaggctc atcacccggt
    12541 aggtaccgcc gtacatgttg ttgccggcca ggacatggtc accggccttc accaggtgga
    12601 gcagcgtggt ggtggccgcc atgcccgagg caaaggcaaa gccgtggtcg gccttctcga
    12661 gcgcggcgat gcagcgctcc aggtgatcgc gcgtcttgtt gtgcgtgcgg gcgtactcgt
    12721 atcccttgtg gtccccgatc ccctgctgca cgtaggtgga ggtctggtag atcggcggga
    12781 tgatggcccc cgtcgtcgga tccggctcct ggccgatgtg gatggcgtcg gtggaaaaac
    12841 ccatgtgtac ctcccgggac ggcctggccg cccgcgcgtc agaaggcccg
//
LOCUS       DASVOD010000575         3263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_714331, whole genome shotgun sequence.
ACCESSION   DASVOD010000575 DASVOD010000000
VERSION     DASVOD010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714331"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..876)
                     /locus_tag="VF720_12720"
     CDS             complement(1..876)
                     /locus_tag="VF720_12720"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880269.1"
                     /translation="MNLKTARYLFGEGIQAVRRHPALSLSAVVAMTASLLVLGGFLII
                     SANVHRIVNDLEDRKEVIVYLKPDQDPSARLRIEERLKDVPGVTGVRYISPEEAWDEF
                     TAEMTGEGLLEEIGDNPLPPSFELKLRADRRNLASIEAIAGEVEAWDEVDEVSYGGAW
                     VSQLDAFARQLAWLNLGVLLAVGLAVVAVVANTIRLTVLAKRDTIEVMKVVGASEWFI
                     RMPFLYEGMFQTLAASVVSLTALYAITRGVSQHFGGISYLTLPQIAAFLGTALLLGMV
                     GSYLALRQVFKGYPV"
     gene            complement(873..1562)
                     /locus_tag="VF720_12725"
     CDS             complement(873..1562)
                     /locus_tag="VF720_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7880270.1"
                     /translation="MIKFYHVYKAYTGGSFALTDINLTIQDGEFLFLVGPSGAGKSTL
                     LKMIYMEALPTDGIVTVGRFTSSGINRRKILELRRHVGVIFQDFRLLRDRNILENVAF
                     PLRIIGEASESEMKERAAAALNEVGVYHRRHALPRELSGGEQQRAAIARAVVTKPFVL
                     VADEPTGNLDPQTSDDITALLKRMNAHGTSVLFATHKMDLVERSTERYLRVEHGQIVE
                     DWVGSGRGATA"
     gene            complement(1642..1917)
                     /locus_tag="VF720_12730"
     CDS             complement(1642..1917)
                     /locus_tag="VF720_12730"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEX7880271.1"
                     /translation="MRVDVWLHKVCLMKSRSMAGEACQRGKVTLDGGAAKASREIRPG
                     NRISIDLGRGILELEVTAVPAGNIPKARAAEYYRVLHDARTTFDDFQ"
     gene            complement(1931..3262)
                     /locus_tag="VF720_12735"
     CDS             complement(1931..3262)
                     /locus_tag="VF720_12735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7880272.1"
                     /translation="MMKNKSGMRVSMVGAALSAAIVLSAEIFLAIPVSSAPKPIEKVV
                     AVVNDEPIMASEVDEQLALLVASRRVDPADTAMVSEARHQILDQLIEERLIVDYAGKK
                     GITVTDEQIEPQVQAAMDEVKARVGDDEKFKTELAKEGMTIDTLRDRYRVDIRKEAMA
                     QRIIEREIRSQVKVTETDVDTFYKNNKAQLPVKPATFNLAHILIIPRPDDARRAGARA
                     RSEKALKRLEAGEAWEKVAAEESEDPNSAPNGGMLGEAKEGDFDPAFEAAIKDLSPGE
                     RTGVVETPAGFHIIQLVARQGVTYQARHILSLAQPSPEDVGRAIERAKVVRADAKKGM
                     DWGALVKANSDDSSTRDDEGKLGEVPASRLGRSYVEALDSLSVGGISEVIQGPTGFHV
                     FRMLGKSEGGEYTYADIKEQLTNMLTQRKLAEAYEKWIADLKKKAFVEYKS"
BASE COUNT          506 a         1093 c         1049 g          615 t
ORIGIN      
        1 tcacaccggg tagcccttga agacctggcg cagcgcgagg tacgagccga ccatcccgag
       61 caggagcgcc gtgcccagga aggccgcgat ctgcggcagc gtgaggtacg agatgccgcc
      121 gaagtgctgc gagacgccac gcgtaatggc gtacagcgcg gtgagcgaca ccaccgaggc
      181 ggccagcgtc tggaacattc cctcgtaaag gaaaggcatc cggatgaacc actccgaggc
      241 gccgacgact ttcatgacct cgatcgtgtc gcgcttggcc agcacggtaa gccggatcgt
      301 gttggccacc acggccacca cggccagccc gacggccagg aggacgccga ggttgagcca
      361 ggccagctgg cgggcgaagg cgtcgagctg gctgacccag gcgccgccgt acgacacttc
      421 gtccacctcg tcccaggcct ccacttcgcc ggcgattgcc tcgatggagg ccaggttgcg
      481 tcgatcggcg cggagcttca gttcgaacga cggcggcagc gggttgtccc cgatctcctc
      541 gagcagcccc tcgccggtca tttcggcggt gaactcgtcc caagcctcct cggggctgat
      601 gtaacgcacg cccgtgacac ccggcacatc cttgagcctc tcctcgatcc gaagtcgcgc
      661 cgacggatcc tggtcgggct tcaggtagac gatgacttcc ttccgatcct cgagatcgtt
      721 gacgatgcgg tgcacgttgg cgctgatgat caggaagccg ccgagcacga ggagggaggc
      781 ggtcatggcc accacggccg agagcgagag cgccgggtga cgccgcacgg cctggatccc
      841 ctcgccgaag agatagcgcg cggttttcag gttcatgccg tggctccccg tccgctaccc
      901 acccagtctt ccacgatctg gccgtgttcc acccggaggt agcgctcggt ggagcgctcg
      961 accaggtcca tcttgtgtgt ggcgaagagc accgaggtgc cgtgcgcgtt catgcgcttg
     1021 agcagcgccg tgatgtcgtc ggaagtctgc gggtcgaggt tgccggtggg ctcgtcggcc
     1081 accagcacga acggcttggt gacgaccgcc cgcgcgatgg ccgcccgctg ctgctcgccg
     1141 cccgagagct cgcgtggcag cgcgtgccgg cggtgataca cgcccacctc gttgagggcg
     1201 gcggcggccc gttccttcat ctcggactcc gaggcctcgc cgatgatacg gagcgggaag
     1261 gccacgttct cgaggatgtt gcggtcgcgc aggaggcgga agtcctggaa gatgaccccc
     1321 acgtggcggc gcagctcgag gatcttcctg cggttgatgc cggaggaggt gaagcgcccg
     1381 accgtgacga tgccgtcggt gggaagcgcc tccatgtaga tcatcttgag gagggtggac
     1441 ttcccggcgc cggagggacc cacgaggaag aggaactcac catcctggat ggtgaggttg
     1501 atgtcggtca gcgcgaagct gccgcccgtg taggctttgt acacatgata gaacttgatc
     1561 atgcgccgct accgcatccg ccttcccccc ccatgttccg ccgtggcggg cggactgtat
     1621 cacgggaagg cgtggccggc ctcactggaa gtcgtcgaac gtggtccggg catcgtgcag
     1681 aacgcgatag tactcggccg cgcgcgcctt cgggatgttc cccgccggca ccgccgtcac
     1741 ttccagttcc aggatgccgc ggcccaggtc gatggaaatc cggtttccgg ggcggatttc
     1801 ccggctggcc ttggccgcgc cgccatccag ggtgaccttc ccccgctggc aggcttcgcc
     1861 ggccatgctg cggctcttca tcaggcagac cttgtgaagc cagacatcga cgcgcaccga
     1921 tcggatcccc tcaggacttg tactccacga aggccttctt cttgaggtcg gcgatccact
     1981 tctcgtacgc ctcggcaagc tttcgctggg tgagcatgtt ggtgagctgc tccttgatgt
     2041 ccgcgtaggt gtattcgccc ccttcggact tccccagcat cctgaagaca tggaagccgg
     2101 tcggcccctg gatgacctcg ctgatcccgc cgaccgagag ggaatcgagc gcctccacgt
     2161 aggaacgccc gagccgggag gccggcacct cacccagctt cccctcgtca tcgcgcgtgc
     2221 tcgaatcgtc ggagttggcc ttcaccaggg ccccccagtc catccccttt tttgcgtcgg
     2281 cccgcaccac tttggcccgt tcgatggccc ttccgacatc ttccggcgag ggctgggcca
     2341 ggctgaggat gtggcgggcc tggtaggtga caccctggcg cgccaccagc tggatgatgt
     2401 ggaagccggc cggcgtttcc acgacccccg tgcgctctcc gggagagagg tccttgatgg
     2461 ccgcctcgaa ggccgggtcg aaatcacctt ccttggcctc gccgagcatg ccgccgttgg
     2521 gggcactgtt cgggtcttcg ctctcctcgg ccgccacctt ttcccacgct tccccggcct
     2581 ccaggcgctt gagcgccttt tccgaccggg cccttgctcc ggcccgccgg gcgtcatcgg
     2641 ggcggggaat gatgaggatg tgggccaggt tgaacgtggc cggcttcacc ggcaactggg
     2701 ccttgttgtt cttgtagaag gtgtcgacat cggtttccgt caccttcacc tggctccgga
     2761 tttcccgctc gatgatgcgc tgggccatgg cttccttgcg gatatccaca cgatagcggt
     2821 cgcgcagcgt gtcgatggtc atcccttcct tcgccagctc cgtcttgaac ttctcgtcgt
     2881 cgccgacgcg cgccttgacc tcgtccatgg ccgcctgcac ctgcggctcg atctgctcgt
     2941 cggtgacggt gattcccttc ttgccggcgt aatcgacgat gagccgttcc tcgatcagct
     3001 ggtcgaggat ctggtggcgc gcctcggaca ccatggccgt atcggccgga tcgacgcgcc
     3061 gtgacgccac gagcagggcg agctgctcat cgacctcgct ggccatgatc ggttcgtcgt
     3121 tgacgacggc caccactttc tcaatcggtt tcggggccga tgaaactgga attgcgagga
     3181 agatttccgc ggacagcacg atagccgccg acagcgccgc gccgaccatc gaaaccctca
     3241 ttccactctt gttcttcatc atc
//
LOCUS       DASVOD010000576         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_507357, whole genome shotgun sequence.
ACCESSION   DASVOD010000576 DASVOD010000000
VERSION     DASVOD010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507357"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1209
                     /locus_tag="VF720_12740"
     CDS             178..1209
                     /locus_tag="VF720_12740"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEX7880273.1"
                     /translation="MSRPVGTAAELERRRTQAVQAVTGGETRNTVAKVLGVHYRTVAR
                     WVRQAQKPGGVGAKPQHRPEPGLSDADLRKLERLMAKGAKAHGWHNQLWTAARVARLI
                     EQHFDISYHPEHVRKILKRRLRWTSQKPRRKARERNDKEVARWVGDEFPRVVREAFRR
                     RAYIAFLDESGFFLTPTVRRTLAPRGKTPVLDAWDRRDRLSAISCVTVSPVAGRPGLY
                     FDLLDHNVHGEDVVEFLAELHRRLGRVTVVWDRNNIHSRAKAVKAWLAGHPDVVVEDF
                     PGYVPDLNPDEGVWGWTKYGRLANLAAADKDELWGRVADELVALKFRPDLLRGFIRQT
                     GLPVVSAAA"
     gene            complement(1166..2461)
                     /locus_tag="VF720_12745"
     CDS             complement(1166..2461)
                     /locus_tag="VF720_12745"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX7880274.1"
                     /translation="MDPLPESVIQTMRSAAALLCGDKRRRFQALVARDHCGGSARIAE
                     RLFCWGRESVETGLGELRTGITCLVNSVYRGRRGIEVRHPGILTELELLLANDTAGNP
                     MCEEKWVRGSLDHLSRQLKEKGYKVGCKAVRRILRGMGYSRKSNRRRQPRPHCPTRNS
                     QFEYIALQRRKFTTAALPVISIDTKKKELIGAFRNKGQTWCKDAEEVDEHDFPGASQR
                     KAVPFGIYDPARNTGHVVVGASNNTPEFATSSIDQWWVTKGCLAYPGAKELLILADGG
                     GGNGSRARAWKLNLQTRICDKHRLTVTVCHYPPGCSKWNPVEHRLFSYISTNWAGKPL
                     RSLDIMLAYIRGTTTRTGLAVTAVLDEATYKKGQKVTKKELDALNLRWHDVLPDWNYT
                     LTPRDPVARELAGDSSVRTGRGGVPVTRPPTRRGGRSGG"
BASE COUNT          420 a          838 c          882 g          471 t
ORIGIN      
        1 gtggcagcgg ctcccggtcg gcgtcacgaa gctgatcggg ccggcgatcg tgcgcgggat
       61 tccgtaagga ttgcaccgcc gcgagcggcg gaagatggtg gcacctcccc acccccgggg
      121 ttactgggtc cagcctcgat aacgcaccct cgctccgctc agcacatcta acctggcatg
      181 agcagacctg ttggcaccgc tgccgagttg gaacgccgcc ggacgcaggc ggtccaggcc
      241 gtgaccgggg gcgagacccg caacaccgtg gcgaaggttc tcggtgtcca ctacaggacc
      301 gtggcccggt gggtccggca ggcccagaag ccgggcggag tgggggccaa gcctcaacac
      361 cgccccgaac ccggcctgtc cgatgccgac ctgcgaaagc tcgaacgcct gatggcgaag
      421 ggggcgaagg cccacgggtg gcacaaccaa ctgtggactg ccgcccgggt cgcccgcctg
      481 atcgagcagc acttcgacat ctcctaccac cccgagcacg tccgcaagat cctaaaacgc
      541 cggctccggt ggaccagcca gaagcccagg cggaaggccc gggaacgcaa cgacaaggag
      601 gtggcaaggt gggtgggtga cgagttcccc cgggtcgtgc gggaggcgtt ccgccgccgg
      661 gcgtacatcg ccttcctcga cgagtcgggt ttcttcctca ccccgaccgt ccgccgcacc
      721 ctcgccccga ggggcaagac cccggtcctc gacgcctggg accggcggga ccggttgagt
      781 gccatcagtt gcgtcaccgt cagcccggtg gccggccggc cgggattgta cttcgacctg
      841 ctcgaccaca acgtccacgg cgaggacgtg gtcgagttcc tggccgagtt gcaccggcgg
      901 ctggggcgag taaccgtggt ctgggaccgg aacaacatcc acagccgggc gaaggcggtc
      961 aaggcgtggc tggcaggcca cccggacgtg gtagtcgagg acttccccgg gtatgtgccc
     1021 gacctgaacc cggacgaggg agtgtggggg tggacgaagt acggacggtt ggccaacttg
     1081 gcagcggccg acaaggatga gttgtggggt cgggtcgcgg acgagttggt ggcgttgaag
     1141 ttccgcccgg acctgctccg ggggtttatc cgccagaccg gcctccccgt cgtgtcggcg
     1201 gccgcgtgac ggggacacca cctcggccgg tgcggacaga cgagtcgccg gccagttctc
     1261 gggcaaccgg gtcgcggggg gtgagcgtgt agttccaatc ggggagcacg tcgtgccagc
     1321 gtaggttcaa cgcgtccagc tctttcttcg tgaccttctg ccccttcttg tacgtcgcct
     1381 cgtccagtac cgccgtcacg gccaggccgg tgcgagtcgt tgtccctcgg atgtaggcga
     1441 gcatgatgtc gaggcttctg aggggcttgc ccgcccagtt cgtgctgatg tagctgaaga
     1501 gtcggtgttc gaccgggttc cactttgaac agccgggtgg gtagtggcag acggtaacag
     1561 taagcctgtg cttgtcgcag attcttgtct ggaggttgag cttccaagcc cttgcgcggc
     1621 tgccattgcc gccaccacca tcagccagga tgagcaactc cttcgccccg gggtatgcca
     1681 ggcatccctt ggtgacccac cactggtcga tcgaactcgt cgcgaactcc ggagtgttgt
     1741 tcgacgcccc cactacgaca tggcccgtgt tccgggccgg gtcatagatc ccgaacggga
     1801 ccgccttacg ttgcgaggca ccggggaagt cgtgttcgtc cacctcctcg gcgtccttgc
     1861 accacgtctg ccctttgttc cggaacgccc cgatgagctc cttcttcttc gtatcgatgc
     1921 tgatcacggg gagtgcggcc gtcgtgaact tccgccgctg tagggcgatg tactcgaact
     1981 gcgagttccg ggtggggcag tgcgggcgcg gctgccgcct tctgttgctc tttcgggagt
     2041 agcccatccc gcggaggata cgcctgaccg ctttgcagcc caccttgtaa cccttctcct
     2101 tcaactgccg actgaggtga tcgaggctgc cacgcaccca cttttcctcg cacatcgggt
     2161 ttccggcggt atcgttggca agtagtagct ccaactcggt gaggatgccg ggatgccgca
     2221 cctcgatccc gcgtcgtcca cgatacactg agttgacaag gcacgtaatg cccgtgcgca
     2281 actcgccgag gcccgtctcg acggattctc gaccccagca gaaaaggcgt tcggcgattc
     2341 gtgcactccc gccgcaatgg tcgcgggcaa ccagtgcctg gaagcggcgg cgcttgtcgc
     2401 cgcagaggag tgcggcagcg gaacgcatgg tctgaatgac tgattcgggc agggggtcca
     2461 tagtaccgcc cttcagatcg ggcctgtagc acggtcatga taccggccga tggcctgagc
     2521 gcggggaggg ttccttccgg atgctggacg cagtagtggt agtatccccg gcggctgcta
     2581 agctttctgt tcggcggcgg gcgtcggggg t
//
LOCUS       DASVOD010000577         8107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_767176, whole genome shotgun sequence.
ACCESSION   DASVOD010000577 DASVOD010000000
VERSION     DASVOD010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767176"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF720_12750"
     CDS             <1..345
                     /locus_tag="VF720_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX7880275.1"
                     /translation="LLGAFTLFWVAGFDIIYATQDVEFDRKAGLFSIPSRLGVNRALA
                     VSGWLHVAAFLALALLVWWQLRTPVAALFLAATGVLLLLEHRQGQNIETAFFRINAAL
                     GFVVLALVVAGT"
     gene            342..893
                     /locus_tag="VF720_12755"
     CDS             342..893
                     /locus_tag="VF720_12755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEX7880276.1"
                     /translation="MRILIGISGASGSTVGAAFVCRCPGDKFLVVSEWGWKNLDIEAG
                     LSRADLEPHVKRIFDDNDLAAPPSSGSNIFDATVIVPTSLSTLAKIAAGLADTLITRM
                     AQVALKERRKLVICLRETPLSSIALENALKLSRDGAMIMPVCPTFYRDPKSVDELVDG
                     YVDKVLMAIGAAQPKGWRSELLD"
     gene            890..1843
                     /locus_tag="VF720_12760"
     CDS             890..1843
                     /locus_tag="VF720_12760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880277.1"
                     /translation="MIAALLVLLAVFDFQKSSAVDLFFTARARAAAAGYVPSGPKLQK
                     AVDEVRRCEVELGGSAEWGIIEGALAGCETAADIKRTFGFFPEAYAYGDRSLKLRSAT
                     LKLATALEGAEKEFLADVWPKHLATLNTKEASLTKTLGPGEKIWLGHLVTYLDFEMPT
                     KPALMYLVAEAPEPWGFTRLDRGSQPVIFLGVGNATGSTLAELAVHELIHALDARQGA
                     DRGALRELRIHLNEAGIATGSSESSRICHALMYIASGEVIKRIVSGQHHHFGETYGTY
                     GRMPEVAPSLLEWWRRYLDGTIDREEMLASFVREYVAAKKP"
     gene            1840..3621
                     /locus_tag="VF720_12765"
     CDS             1840..3621
                     /locus_tag="VF720_12765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEX7880278.1"
                     /translation="MTAPPGRPPFDDLQAFAAHLEARGELRRIRVEVDPLHEIPEIAF
                     RAVKEGLPALLFERPAGSDVPVLLNMLATDARIEMALGRPPGAFGEMLGRMAHRLMPP
                     KPANLWGERSTLLRALNMRKARVGSGPVLDATTSPDLDRLPILKLWPDDGGRFITFPL
                     VLTADPETGVGNMGVYRMHVYDKASTGMHWQIQKGGGFHYHKAEAAGRPLPVCVALGG
                     DPILLLCAVAPLPEGIDEIAFAGFLRGRPVKMVRGPSTGLLAPADAEFLIEGQVAPVE
                     RRVEGPFGDHFGHYSHAAPFPVFRIQSISRRKNAIYPAAVVGKPPQEDKFIGNALQEM
                     TLPILRIMHPEVIDAWAYYETGFHSLFVVSVRERYRKEAVKTALGLLGTGQLSLTKVM
                     VTVGPEVNVRDFPAVLEAIRDHFDPVEDFLLLPGTPFDTLDFTSFKMNMGSKMIIDAV
                     ERADVRPNEPREFDTFDAAAVHHRVERWRLLEDALLVVQVERDPWPVLEAFQALPRDA
                     APPLVVAVSPDVELADLESLLWGIFTRFEPARDVRFTESRMVGAQPRWEGRMLVDATW
                     KNGYPSPVLMDPDIVRRVDRRWSEYWK"
     gene            3618..4733
                     /gene="mqnE"
                     /locus_tag="VF720_12770"
     CDS             3618..4733
                     /gene="mqnE"
                     /locus_tag="VF720_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007545439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEX7880279.1"
                     /translation="MTTIRDPKLIPIAEKVKAGERLTREEGLDCLSTPDVLSLGRLAD
                     TVKRRKSGNYVYYVFNRQINPTNVCVLSCRFCDFATTKQAPNAYEMTLTDILAKCRGG
                     IREVHIVGGMHPDWPYEKYVDIIRQIRAHYPDITIKAYTAVEIDWFAEISGKSIEQVL
                     DDLVAAGLDTIPGGGAEVFSERVHQIMYKPKIGADRWLAVHRAAHKKGIPTNATLLYG
                     HIETFEERIDHLVQLRELEDEAPGFLSFIPLAFQPGHTGIVERQTSAIDDLRMIATSR
                     LMLDNFPHIKAYWVMVGEDTASVALNFGADDIDGTIGEERIAHAANASSPLGLTRQRL
                     LHLIREAGKVPVERDAKYHVITVHDQHADRPDPVPQH"
     gene            4675..5472
                     /locus_tag="VF720_12775"
     CDS             4675..5472
                     /locus_tag="VF720_12775"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEX7880280.1"
                     /translation="MSSPSTTSTLIARIPYLNTEPFYRTFQATGYELLDLPPRQLGEA
                     AAKGRVVAGPMSLCDWLNLEEQFEPLGPYGIASLGAVRSVLLFSRKPIEELAGGSIAI
                     TEETSTSVRLLEVLFRDYWGVRPAVWRRGTTEPTDAMLLIGDAALRARAARLSGAMSE
                     YPVMVDLAAAWSEWQKKPFVFALWVVKRDLPLEEKKILSMRFEDSLYEGLKAIPIIAT
                     ERSADLGLPPDELEAYLRHFRYRLGPDDWEGLNTFRRLIENVRHADG"
     gene            5450..6535
                     /gene="mqnC"
                     /locus_tag="VF720_12780"
     CDS             5450..6535
                     /gene="mqnC"
                     /locus_tag="VF720_12780"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013536914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEX7880281.1"
                     /translation="MSATLTDDITARVLDGARLTVEEGLHLLRDAELLDLGNLAHHVR
                     MQKHPHDYVTFVIDTNPNYTNACTTDCQFCAFYRKPGHSDVYVHPVREMVEIIRRAVG
                     QGATTLLLQGGHNPDIPYSYYLEMVEALHAEFPKLALHLWSASEIHQMAEVSGKTTGE
                     VLQDMWDRGQRSMPGGGAEVLTDRVKKRISPKKITTAQWLDVHREAHRIGMKTTTTMM
                     YGHVETDEDIIEHLDVCRRLQDEYQGFTAFVPWSFKPSNTLMMPKQTAVAGPARYLKI
                     LAVSRLFLDNIPHIQASWFSEGKKTGQIALHFGADDFGGTLIEERVLAAADHHNRTST
                     DEVMAIIQESGFRPAQRTTLYEVLERF"
     gene            complement(6871..>8107)
                     /locus_tag="VF720_12785"
     CDS             complement(6871..>8107)
                     /locus_tag="VF720_12785"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX7880282.1"
                     /translation="VRPNGGPALRGEVSINSRVFVSLRSPLGFDLVAGQSVAFDVVAD
                     DVRGGVPTLAATGLPAGATFAENGNGTGHFAWTSPAGVSSQHDVVITATGAGGRIDRS
                     TTHLSAIRPNNAPVAEANGPYAGETGAAVALSSVGSSDPDLDVLGYLWDFGDGQTSND
                     ASPSHAWAAPGTYTVRLRIGDGQATAVDSATAVITDPAPTSTVARAFLTGGNRDFKPF
                     ANRPWFSVQMERADRAFLAGEVDAASLVMTSKGTGSIEQVSANAQRTVMMADTDGNGR
                     GELAATFDSEQLKRLFDTITRPTIVPVTIEGLLANGQTVTAELTLSVTPKPGSPVKTK
                     GFGSTVGLIVEQATSGRLAVRLFDTAGRLVREVVNADRPAGVHEFDLSSGGTLPAGLY
                     FYRVSVDGEVSAGKLMIRP"
BASE COUNT         1357 a         2798 c         2626 g         1326 t
ORIGIN      
        1 ctgctcggcg cgttcaccct cttctgggtg gccggcttcg acatcattta cgccacgcag
       61 gatgtcgaat tcgaccggaa ggccggcctc ttctcgatcc cctcacgtct cggcgtgaat
      121 cgcgcgctgg ccgtgtcggg atggctgcac gtggccgcgt tcctcgcgct ggcgctgctc
      181 gtctggtggc agctccgcac accggtggcc gcgcttttcc tggccgccac gggtgttctc
      241 ctcctactcg agcaccgaca ggggcagaac atcgaaacag ccttcttccg gatcaacgcg
      301 gcgctcggct tcgtcgtgct cgcgctggtg gtggccggca cgtgaggatc ctgatcggca
      361 tctccggcgc ctccggcagc acggtgggcg ccgcattcgt gtgtcgctgc cccggcgaca
      421 agttcctcgt ggtcagcgag tgggggtgga agaacctcga catcgaggcc gggctttccc
      481 gcgccgacct cgagcctcac gtgaagcgga tcttcgacga caacgacctg gccgcgcccc
      541 cgagctccgg cagtaacatc ttcgacgcca ccgtgatcgt gccgacctcg ctctccacgc
      601 tggccaagat tgctgccggc ctggccgaca cgctcatcac ccggatggcc caggtggcgc
      661 tcaaggagcg acggaaactg gtgatctgcc tccgggaaac gcctctctcc tcgatcgcgc
      721 tcgaaaacgc cctcaagctc tcgcgcgatg gggcgatgat catgccggtc tgtcccacgt
      781 tctaccgtga cccgaagtcg gtggacgagc tggtcgacgg ctatgtcgac aaggtgctca
      841 tggccatcgg cgccgcccag cccaaggggt ggcgatcgga gttgctggat tgatcgcggc
      901 actcctcgtc ctccttgccg tgttcgactt ccagaagagc tcggccgtcg atctcttctt
      961 cacggcgcgc gcacgggcgg cggcggcggg ttacgttccg agcggtccaa agctccagaa
     1021 ggcggtggat gaggtccgtc gctgcgaggt ggagctgggc ggcagcgccg agtggggaat
     1081 catcgaggga gcgttggccg gttgcgaaac ggcggccgac atcaagcgga cgttcggctt
     1141 cttccccgag gcgtacgcgt acggcgaccg ctcgctcaag ctccgctcag ccacgctcaa
     1201 gctggccacc gcgctggaag gggccgaaaa ggagttcctg gccgacgtct ggccgaagca
     1261 ccttgccacg ctgaacacga aggaagcctc cctcacgaag acgctcggtc ccggcgagaa
     1321 gatctggctc gggcacctgg tcacctatct cgacttcgag atgccgacga agccggcact
     1381 catgtatctc gtggcggagg ctcccgaacc gtggggattc acccgcctcg accgggggag
     1441 ccagccggtc atcttcctgg gcgtcggcaa cgccaccggc tccacgctgg ccgagctggc
     1501 cgtgcacgaa ctgatccacg cgctcgacgc gcggcaggga gccgaccgcg gcgcgttgcg
     1561 ggaactccgc atccacctga acgaagccgg gatcgccacc ggctcgtccg agagcagtcg
     1621 catctgccac gcgctcatgt acatcgcctc gggcgaagtg atcaagcgca tcgtgtccgg
     1681 ccagcatcac catttcgggg aaacctacgg cacctacggc cggatgcccg aggtggcgcc
     1741 ttcgctcctc gagtggtggc gccgctacct ggacggcacg atcgaccgcg aggagatgct
     1801 ggccagtttc gtgcgggagt acgtggcggc gaaaaagccg tgacagcacc gcccggccgc
     1861 ccccctttcg acgacctcca ggcattcgcc gcgcatctcg aagcccgcgg cgaactgcgt
     1921 cgcatccggg tggaagtcga ccctctgcac gaaatccccg agatcgcctt ccgcgcggtg
     1981 aaggaagggc tgccggccct tctcttcgaa cgtcccgccg gcagcgacgt gccggttctc
     2041 ctcaacatgc tggccaccga cgcccgcatc gagatggccc tcggccgccc cccgggcgcc
     2101 ttcggcgaaa tgctcggccg gatggcccac cgcctgatgc cacccaagcc ggccaacctc
     2161 tggggcgagc gctccacgct cctgcgcgcg ctcaacatgc ggaaggcgcg cgtcgggtcg
     2221 ggaccggtac tcgacgccac caccagtccc gatctcgacc gtctccccat cctgaaactc
     2281 tggccggacg acggcgggcg gttcatcacc tttccgctcg tgctcacggc cgatccggag
     2341 accggcgtcg gcaacatggg cgtgtaccgc atgcacgtgt acgacaaggc ctcgaccggc
     2401 atgcactggc agatccagaa ggggggcggt tttcattacc acaaggccga agcggccggg
     2461 cggccgctgc cggtgtgcgt ggcgctcggc ggtgatccga tcctcctcct gtgcgcggtg
     2521 gctccgctgc cggaggggat cgatgagatc gcgttcgccg gattcctgag ggggcgcccg
     2581 gtcaagatgg tgaggggccc ctccacgggg ctcctggcgc ccgccgatgc cgagttcctg
     2641 atcgaggggc aggtggcgcc ggtcgaacgt cgcgtggaag ggccgttcgg cgatcatttc
     2701 ggccactaca gccacgccgc gccgttcccg gttttccgca tccaatcgat cagccggcgg
     2761 aagaacgcca tctacccggc cgccgtcgtc ggcaagccgc cgcaggaaga caagttcatc
     2821 ggcaatgcct tacaggagat gacgctgccg atcctccgca tcatgcaccc cgaggtcatt
     2881 gacgcctggg cctattacga gaccggtttc cacagtcttt tcgtcgtttc ggtgcgggag
     2941 cgctatcgca aggaggcggt gaagacggcc cttggcctcc tcggaaccgg gcagctctcg
     3001 ctgaccaagg tgatggtgac ggtgggcccc gaggtgaacg tccgcgattt ccccgcggtc
     3061 ctcgaggcca tccgcgacca cttcgatccg gtcgaggact tcctgctgct gcccggcacg
     3121 cccttcgaca cgctcgactt cacctcgttc aagatgaaca tgggaagcaa gatgatcatc
     3181 gacgcggtcg agcgtgccga tgtacgcccg aacgagccgc gcgagttcga taccttcgac
     3241 gcggccgccg tgcatcaccg ggtcgaacgc tggcgcctcc tggaagatgc gctcctggtc
     3301 gtgcaggtgg agcgggaccc gtggccggtc ctcgaggcgt tccaggccct gccgcgcgac
     3361 gcggcgccac ccctggtcgt ggccgtatcg cccgacgtgg agctggccga cctggaaagc
     3421 ctcctctggg gaattttcac gcggttcgag ccggcccgcg acgtccgctt caccgaaagc
     3481 cgcatggtcg gggcccagcc ccgctgggag gggcgcatgc tggtcgacgc cacctggaaa
     3541 aacggctatc cttcaccggt tttgatggac cccgacatcg tgcgccgcgt ggaccggcgc
     3601 tggagcgagt actggaagtg acgacgatcc gagacccgaa actgatcccc atcgccgaga
     3661 aggtcaaggc gggggagcgc ctgacccgcg aggaggggct cgactgtctc tccacgcccg
     3721 atgtcctctc ccttggccgc ctggccgaca cggtgaagcg ccggaagtcg ggcaattacg
     3781 tttattacgt cttcaaccgg cagatcaacc cgaccaacgt ctgcgtcctg tcctgccgct
     3841 tctgcgattt cgccacgacg aagcaggcgc ccaacgccta cgagatgacg ctgaccgata
     3901 tcctggccaa gtgccggggc ggcatccgcg aggtgcacat cgtcggcggg atgcaccccg
     3961 actggccgta cgagaagtac gtggacatca tccggcagat ccgggcccac tatcccgaca
     4021 tcacgatcaa ggcctacacg gcggtggaga tcgactggtt cgccgagatc agcgggaagt
     4081 cgatcgagca agtgctcgac gacctggtgg cggccggcct cgacacgatc cccggcggcg
     4141 gcgccgaagt gttcagcgag cgcgtccacc agatcatgta caagccgaag atcggcgccg
     4201 atcgctggct ggccgtgcac cgggctgccc acaagaaggg cattcccacg aacgccacgc
     4261 ttctgtacgg ccacatcgag acgttcgagg agcggatcga ccacttggtc cagctccgcg
     4321 agctcgaaga cgaggcgccc ggctttctgt cgttcatccc gctggctttc cagccggggc
     4381 acacgggcat cgtcgagcgg cagacgtcgg ccatcgacga cctccggatg atcgccacca
     4441 gccgcctcat gctcgacaac tttccccaca tcaaggccta ctgggtcatg gtcggggagg
     4501 ataccgcgtc ggtggcgctc aacttcggcg ccgacgacat cgacggcacg atcggcgagg
     4561 agcgcatcgc gcacgccgcc aatgcttcca gcccgctggg cctcacgcgc cagcgccttc
     4621 tccatctgat ccgcgaggcc ggcaaggtgc ccgtggagcg ggacgccaaa taccatgtca
     4681 tcaccgtcca cgaccagcac gctgatcgcc cggatcccgt acctcaacac tgaaccgttc
     4741 taccggacgt tccaggccac gggatacgaa ctgctcgacc tgccgccccg ccagctcggc
     4801 gaggccgcgg ccaaggggcg cgtggttgcc ggcccgatgt cgctctgcga ctggctgaat
     4861 ctcgaggaac agttcgagcc gctcggcccc tacgggattg cctccctcgg cgcggtgcgc
     4921 tcggtcctcc tcttcagcag gaagccgatc gaggaactgg ccggcggttc gatcgcgatc
     4981 acggaggaaa cctccacgtc ggtgcgcctc ctcgaggtgc tcttccgtga ttactggggc
     5041 gtgcgcccgg ccgtctggcg gcggggcacc acggaaccca ccgacgccat gctcctgatc
     5101 ggcgacgccg cgcttcgtgc ccgcgccgcc cgtctttccg gcgcgatgtc cgagtacccg
     5161 gtgatggtcg acctggccgc cgcctggagc gagtggcaga agaagccgtt cgtctttgcg
     5221 ctctgggtcg tgaagcgcga tctgccgctc gaggagaaga agatcctgtc gatgcgcttc
     5281 gaggactcgc tgtacgaagg cttgaaggcc attccgatca tcgccaccga aaggtcggcc
     5341 gatctcggcc tcccgccaga cgagctcgag gcctacctgc gccatttccg ataccggctc
     5401 ggtcccgacg attgggaggg cctcaacact ttccggaggt tgatcgagaa tgtccgccac
     5461 gctgacggat gacatcaccg cgcgcgtgct ggacggcgcg cgcctcaccg tggaggaggg
     5521 gctccacctc cttcgcgacg ccgagctgct cgacctcggc aacctggccc atcacgtccg
     5581 gatgcagaag catccccacg actacgtgac gttcgtgatc gacaccaacc ccaactacac
     5641 gaacgcctgc accaccgact gccagttctg cgccttctac cggaagcccg gccattcgga
     5701 cgtgtacgtg catccggtca gggagatggt cgagatcatc cgtcgcgcgg tggggcaggg
     5761 ggccaccacg ctcctgcttc aggggggtca caatcctgac atcccgtact cctattatct
     5821 cgagatggtg gaggcgctcc acgccgagtt cccgaaactg gcgctccacc tctggagcgc
     5881 ctccgaaatc caccagatgg ccgaggtttc aggaaagacc accggcgagg tgctccagga
     5941 catgtgggac cgcggccagc gctccatgcc gggcggcggt gccgaagtcc tgaccgatcg
     6001 ggtcaagaag cgcatcagcc ccaagaagat cacgaccgcc cagtggctcg acgtgcaccg
     6061 cgaagcccac cgcatcggca tgaagaccac gacgacgatg atgtacggcc acgtcgaaac
     6121 cgacgaggac atcatcgagc acctcgacgt ctgccgccgc ctccaggacg agtaccaggg
     6181 cttcaccgcc ttcgtcccct ggtccttcaa gccctccaac accctgatga tgcccaagca
     6241 gaccgccgtg gccggcccgg cccgctacct caagatcctg gccgtgagcc gcctcttcct
     6301 cgacaacatc ccccacatcc aggcctcctg gttcagcgag gggaagaaga ccggccagat
     6361 cgcgctccac ttcggcgccg acgacttcgg cggcacgctc atcgaggagc gggtcctcgc
     6421 ggccgcggat caccacaatc ggacctccac cgacgaggtg atggcgatca tccaggagtc
     6481 gggattccgg ccggcgcagc ggacgacgtt gtacgaggtg ctcgagcggt tctagggttt
     6541 cggcgatcgg ttgggggtcg attgcgccgg cggcgtgtcc cgtgattggt cggctggtcc
     6601 ggcgcacccg ccctcgatgg acctgtattc gaaacagtca acaggttccg gaacaggttg
     6661 gcgcccgacg tccggcgcgg gttatcgggg gccttgcaag aaagggctcc cgtaccgctt
     6721 gtgaaaaatg gtgcaactcg caccggtgcg agtccaatcg tccctgcaag ccttgatccc
     6781 gtcccaaaaa tccgggcccc gtcatcgccg ggggaaggcg gaacggggcc ctcttgccga
     6841 atggcgggaa ggggcccgcc acccgtcacg ctacggccgg atcatcagct tgccggccga
     6901 cacctctccg tccaccgata cccggtagaa gtaaaggccg gccggaagcg ttccacccga
     6961 cgaaagatcg aactcgtgca cgccggcggg tcggtcggcg ttgaccacct cgcggacgag
     7021 gcgccccgcc gtgtcgaaga ggcgtaccgc caggcgaccg gaggtggcct gttccacgat
     7081 gaggccaacg gtcgaaccga atcccttggt cttgaccggc gagcccggct tgggcgtcac
     7141 cgagagagtc agctcggccg tcacggtctg accgttggca aggagccctt cgatcgtgac
     7201 gggaactatc gtcggacgcg tgatcgtgtc gaacagtcgc ttgagctgtt ccgaatcgaa
     7261 cgtggcggcc agctcgccgc gcccgttgcc gtccgtgtcc gccatcatca cggtgcgctg
     7321 ggcgttggcg gagacctgct cgatcgagcc ggttcccttc gaggtcatga cgagcgaagc
     7381 cgcgtccacc tcgccggcca aaaaggcccg atcggcccgt tccatctgca cggagaacca
     7441 gggtcggttg gcaaacggct tgaagtcacg gttgccaccc gtcaggaatg ccctggccac
     7501 tgtgctggtc ggggccggat cggtgatcac ggccgtcgcg gagtcgaccg cggtggcttg
     7561 gccgtcgccg atgcgcaggc ggacggtgta ggtgccgggt gcggcccagg cgtgcgaggg
     7621 actggcatca ttcgaggtct ggccgtcgcc aaagtcccac aggtagccga ggacatcgag
     7681 gtcggggtcg ctcgagccca cgctgctgag cgccaccgcc gcgccagtct cgccggcgta
     7741 ggggccgttg gcctcggcca cgggggcgtt gttcgggcgg atggcggaaa gatgcgtcgt
     7801 ggagcggtcg atgcggcccc ccgcaccggt ggccgtgatc accacgtcgt gctggctcga
     7861 aacgccggcg ggcgacgtcc acgcgaagtg accggtgccg ttcccgtttt ccgcgaacgt
     7921 ggcgcccgcc gggaggccgg tggcggccag ggtcggaacg cccccccgca cgtcgtcggc
     7981 caccacgtcg aaggccaccg actggccggc caccaggtcg aaccccagcg gggagcggag
     8041 cgacacgaag acacgggagt tgatgctcac ctcgccgcgc agcgccggtc cgccgttcgg
     8101 acgcacc
//
LOCUS       DASVOD010000578         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_246777, whole genome shotgun sequence.
ACCESSION   DASVOD010000578 DASVOD010000000
VERSION     DASVOD010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246777"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(458..763)
                     /locus_tag="VF720_12790"
     CDS             complement(458..763)
                     /locus_tag="VF720_12790"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7880283.1"
                     /translation="MSRGPIGRDVPRAQGARNPHRDGAATWAEGACEPERFLVAGDRV
                     LVFVDVRVRLNGKEDWIEGRAVDVFTFRDGKVIHWRTFAEAARALEWAGVPLDSSGA"
     gene            complement(941..2326)
                     /locus_tag="VF720_12795"
     CDS             complement(941..2326)
                     /locus_tag="VF720_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HEX7880284.1"
                     /translation="MIARLAGLLTRWAIRWVPDSFVIAILLTVVVFALTLAVTASTPL
                     QVVQYWGDGLWELLSFGMQMCLVVLTGFVVAVSPPVKRALVAVARIGRTPRQSIAVVA
                     VTSMALAWLNWGLSIVASAMLARNVARRGHGVDYRLLVTTAYLGMGCVWHAGLSGSVF
                     LLVATPGHFMAKEIGVIPVTRTLFSPFNLGLTVVVIAVMTALAVWLHPAPEDAYVARP
                     DEVPDDSDGVEPVAGGGANAGDTVNTGDGLTMVRPSFVQRVERSRLVMLVPVVLGALW
                     IYLNLRSKGPAGFNLNVLNLLFLVLGGLLHGTPASFLRAAEEGGRYLWGIVIQFPLYA
                     GIFGMIKFSELQTVISGWLASFLSAGTYHTFVLWYSGVLNYIIPSGGSKWAIEAPYVL
                     EAAHRLGASIDPVITAYAWGDMMTDIIQPFWAIPLLAITRLQFRDIMGYALVFFLVYA
                     AVVTGAFVLFG"
     gene            complement(2331..>3662)
                     /locus_tag="VF720_12800"
     CDS             complement(2331..>3662)
                     /locus_tag="VF720_12800"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880285.1"
                     /translation="IAPPTAPYWTAMTNRLTAMGLTAAQVQVAWLKEAEAGPPNNFPS
                     HAQALRDTLKKVVRNLHDKFPNLRLVYCSSRIYGGYASQGGLNPEPQAYESGYAVKWL
                     IEDQVNGDPALNYGHYPGPIRAPLLLWGPYLWADGPTPRSDGLVWLPADLEADNVHPS
                     ASGEQKVAALLAAFFASDPTAASWWPAVGGETLVTLDALHDSHVSAAAPGSNFGAATL
                     LTEQGGAQPINIYVRFDASAETRPIKLAKLDFRVSAVGGGFVRRVNDTSWTEGAVTWS
                     SAPPLGATLAAISQATKDGTADANVTADLNGDPDRQVSYALTIPAVSPGTLVSREGGQ
                     APRLVLVVQTATAGVPPPAEARLTASPNPARGATRITYELERAGVVEIAVIDVGGRLV
                     RRLVAGARPAGPGVIGWDGHAEDGSPVSAGLYFVRLESAAGRISRKIVLAH"
BASE COUNT          697 a         1342 c         1053 g          570 t
ORIGIN      
        1 gccctcgccg ccggccgcca gcaactcaac tgggacggcc gctccgacgc gggcttcgcc
       61 gtggcctccg gcgtctacct ggcgcggatc gaggcgccgg ggaacacgag ctggctgcgg
      121 gtgatgcggg cgagatgatc cccaggcggc ggaccattcg ttacctgttc cggaacaggc
      181 tctcttcgtt acatgttccg gaacatgctc tcttcgttac atgttccgga acaggctctc
      241 tcagagaatt ttgacgctct cgcccataaa gatcatgtcg aagaacctga atcggaatga
      301 gtccaggtac tcgcttcgct gaggcgcccc tccgcaaggg cgtccgacat cccggaattc
      361 tacgctgccg ctcggtttac tctccttgag gagagttgcc cgagcacacc ctgcatcccg
      421 aacacaccct gcatcccgag cacacgctgt atcccgatca ggcccccgaa gaatcgagag
      481 gcaccccggc ccactccagt gcccgcgcgg cctccgcaaa ggtgcgccag tggatcacct
      541 tgccatcgcg gaacgtgaag acgtccaccg cccgaccctc gatccagtcc tcttttccat
      601 tcagcctcac ccgcacgtcc acgaacacga ggacccgatc cccggccacg aggaacctct
      661 ccggctcgca cgccccctcg gcccacgtgg ccgcgccgtc gcgatgtggg tttcgagctc
      721 cttgagcccg cggaacgtcc cgcccgatgg gccctcgaga aattcactcc gctcgatctg
      781 cgagtcaaag ggtttcagtg ttgccgggat atcgtttcga ttgagtgcgg aatagagccc
      841 acgcagggac tcgatctcgg cctcgaacgg ctttccaggc tccttcatct tccaggctcc
      901 ttcatcttcc tcgttccttc atgtccctct ccgcagtatg tcatccgaac agcacgaacg
      961 cccccgtcac caccgccgca tataccagga agaacaccag tgcgtacccc atgatgtccc
     1021 ggaattgcag tcgcgtgatc gccaggagcg gaatggccca gaacggctgg atgatgtcgg
     1081 tcatcatgtc gccccatgcg taggcggtga tcacgggatc gatcgacgcc cccagccgat
     1141 gcgcggcctc cagcacataa ggcgcctcga tggcccactt ggaaccaccc gacgggatga
     1201 tgtagttcag cacgcccgag taccagagca cgaacgtgtg atacgtgccg gcactgagga
     1261 acgaggccag ccaccccgag atcaccgtct gcagctcgga aaacttgatc atcccgaaga
     1321 tgccggcata cagcggaaac tggatgacga tcccccacag gtagcgcccc ccctcttccg
     1381 ccgcccgcag gaacgatgcc ggcgtgccgt gcagcaaccc gcccagcacc agaaacaaca
     1441 ggttcagtac attgagattg aatcccgccg gccctttgga cctgagattc aaatagatcc
     1501 acagcgcccc gagcaccacc ggcaccagca tcacgagccg cgaccgctcc acccgctgca
     1561 cgaaggacgg acggaccatg gtcagcccat cgccggtgtt caccgtatcc ccggcattcg
     1621 cacctccgcc cgccaccggc tccaccccat ccgaatcatc cggtacctcg tcgggccttg
     1681 cgacataggc atcttccggt gccggatgca gccacaccgc cagtgccgtc atgaccgcaa
     1741 tcaccaccac ggtgagcccc aggttgaacg ggctgaacag ggtgcgcgtc accgggatga
     1801 cgccgatctc cttagccatg aagtgccccg gcgtggccac cagcaggaac accgagcccg
     1861 agagccccgc gtgccatacg caccccatcc cgagatacgc cgttgtgacc aggagccggt
     1921 agtccacgcc gtgcccgcgg cgcgcgacat tccgcgccag catcgcggag gccacgatcg
     1981 acagccccca gttgagccag gcaagcgcca tcgacgtgac ggccacgacg gcgatcgact
     2041 gacgcggcgt acgcccgatc cgcgccaccg ccacgagcgc gcgcttcacc ggcggcgaca
     2101 ccgcgacgac gaagcctgtc agcacgacga ggcacatctg catcccgaac gagagcagct
     2161 cccacagccc atcaccccag tactgcacga cctggagcgg ggtcgatgcc gtgaccgcca
     2221 gcgtgagggc gaacaccacc accgtcagga ggatggcgat gacgaacgaa tcgggcaccc
     2281 accggatggc ccagcgggta aggagaccgg cgagacgcgc gatcatgcgg tcaatgggcg
     2341 agcacgatct ttcgggagat gcgaccggcg gccgattcga ggcggacgaa gtagagaccc
     2401 gcgctcaccg gtgaaccatc ctcggcgtgg ccatcccacc cgatgacacc cggtccggcc
     2461 ggtcgtgctc ccgcaaccaa ccgccgcacc agtcgtccgc cgacatcgat gaccgcgatc
     2521 tccacgacac cggcccgctc gagctcatac gtgatcctcg tcgctccgcg ggccggattc
     2581 ggtgacgcgg tcaagcgagc ctccgcagga ggcggtacac ccgcggtggc cgtctgcacc
     2641 accagcacca gccgcggcgc ctgccccccc tccctgctca ccaacgttcc cggcgacact
     2701 gccggaatcg tcaacgcgta ggaaacctgc ctgtccggat caccgttcag gtcggccgtc
     2761 acgttcgcat ccgccgtccc gtccttcgtc gcctgcgaaa tggccgccag cgtggcgccg
     2821 agtggcggcg cgctcgacca tgtgacagcc ccctcggtcc aagacgtatc gttcacccgc
     2881 cgcacgaaac cgccgccgac cgccgacacg cggaagtcga gcttggccag cttgatcggg
     2941 cgcgtctcgg ccgatgcgtc gaaccgaacg taaatgttga tcggctgagc accaccctgc
     3001 tccgtcaaca acgtggccgc cccgaaattg gaaccgggcg ccgccgcgct gacatggctg
     3061 tcgtgcagcg cgtcgagcgt caccagtgtt tcgccaccca cggccggcca ccacgaagcg
     3121 gccgtgggat ccgacgcaaa gaacgcggca agaagcgccg ccaccttctg ctcgcccgac
     3181 gcgctcgggt gcacgttgtc cgcctcgaga tcggccggca gccagaccaa tccatccgag
     3241 cgcggcgtcg gtccatccgc ccagagatac ggcccccaca acagcagcgg tgcccgaatc
     3301 ggcccgggat agtgcccgta gttcagcgcc ggatcgccat tcacctggtc ttcgatcaac
     3361 cacttcaccg catagcccga ctcgtacgcc tgcggttcgg gattgaggcc accctgcgac
     3421 gcgtatccgc cgtagatgcg actggaacaa tagacgagcc gcagattggg aaacttgtcg
     3481 tgaagattgc gcacgacctt cttgagcgtg tcccgcaggg cctgcgcgtg cgacggaaaa
     3541 ttgttcggcg gccccgcctc ggcctccttg agccaggcca cctgcacctg cgctgcggtc
     3601 agtcccatcg cggtcaggcg attggtcatt gccgtccagt acggcgcggt cggtggcgca
     3661 at
//
LOCUS       DASVOD010000579         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_662695, whole genome shotgun sequence.
ACCESSION   DASVOD010000579 DASVOD010000000
VERSION     DASVOD010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662695"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="gcvPB"
                     /locus_tag="VF720_12805"
     CDS             complement(<1..993)
                     /gene="gcvPB"
                     /locus_tag="VF720_12805"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX7880286.1"
                     /translation="MDNRLIFEMSSRGRCGYTMPKMDVPAPSNDDEKLLRQFRREKPP
                     ELPEVSEPEVVRHFTNISVLNHHVDKDFYPLGSCTMKYNPKLNDRAAAMPGLADIHPL
                     APEEVSQGALALLHDLKTMLAEITGLPAVTLQPAAGAQGELTAMLMTRRYHMDRGEPH
                     RDKVIIPDSAHGTNPATVRIAGMHVIEIKSNANGLVDVEALKAVLGPDTAAMMLTNPN
                     TLGIFERDVEKITRLVHEAGGLVYMDGANMNALMGVAKPGHMGADVLQFNLHKTFSTP
                     HGGGGPGAGPVAVRSFLEKFLPRPRIVEKNGKPAWDHDCPDSIGKVRSFYGNFGM"
     gene            complement(986..2344)
                     /gene="gcvPA"
                     /locus_tag="VF720_12810"
     CDS             complement(986..2344)
                     /gene="gcvPA"
                     /locus_tag="VF720_12810"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX7880287.1"
                     /translation="MSGTHPYIPGTAADEAAMLAAIGVKKFEDLLAPLPENLRLKELL
                     DIHPGRSEWEVVRDLSAWGNRNAGAGRYTCFLGGGIYDHHIPSVIDHVTMRSEFYTAY
                     TPYQAEVSQGTLIAIYEFQTMVSELTGLAVANASLYDGASAAGEAALLAASVTGRPKT
                     IIAGRFHPNYRDVLKTYVEMSGHQVVVDPAPDHVADLAWLDANLDESTAAVFVQHPNF
                     FGRLEQMDEIRAKAQAKGALFVAVVDPSTLGILAPPGAYGADIAVGECQPLGIPMAYG
                     GPVAGFFAADTKYLRQMPGRLVGETVDKDGKRCWTLTLQTREQHIRREKATSNICTNQ
                     TLLAHRATVYMSLLGPNGMRELGEQCHARASHAARTVSQIPGYSIALPGPHVKEFVVK
                     CPVPAAQVVAEGLRHEMLVGLDLGRFDPAWKDLLLVAVTEKRSADDIAKLGKVLAGVS
                     RG"
     gene            complement(2341..2724)
                     /gene="gcvH"
                     /locus_tag="VF720_12815"
     CDS             complement(2341..2724)
                     /gene="gcvH"
                     /locus_tag="VF720_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX7880288.1"
                     /translation="MTTIPDSLHYTKEHEWIRVEGDVATIGITDYAQGELGDIVYVTL
                     PNKGNEVRQMAAFGTVEAVKTVSDLYAPVSGTIIDVNAALNDAPDSVNKSPYEAGWMI
                     KVKMSAPAELEGLLSSAAYKELIGS"
     gene            complement(2721..3836)
                     /gene="gcvT"
                     /locus_tag="VF720_12820"
     CDS             complement(2721..3836)
                     /gene="gcvT"
                     /locus_tag="VF720_12820"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX7880289.1"
                     /translation="MKVEDHLSLETPFTDRNRALGGKIVDFAGYLMPLQFAGQLKEHE
                     AVRTGVGLFDLSHMGEFHFSGPGALEFTDHLLTNQLSAKEPGGIVYSPMCRPDGGIVD
                     DVLAYRREDGVLLVVNAANIEKDLEWVRSHAPEGMIVHDRSADTALLAIQGPKAGAVV
                     KPFTGDWITDLGYYHFREFLWDGVPVLISRTGYTGEDGFELFFDRRHADTMWERIQEA
                     GKPHDMAPIGLAARDTLRLEVGYCLYGNDIDDTTTPLEAGLQWTVKLDKPDFIGKDAL
                     VTQKAAGLRRKLMGLTIDQAGMIPRAGMNVLTDGTVTGRVTSGTMSPSLKKSIAMAYV
                     PIGSAKPGSEVQVDIRGRTARAVVQRLPFYTEGTRKS"
     gene            complement(3914..>5186)
                     /locus_tag="VF720_12825"
     CDS             complement(3914..>5186)
                     /locus_tag="VF720_12825"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX7880290.1"
                     /translation="VLLVGCGDAAKFDAVRARKAGVRVARAAHEQKMKKITAGGVPLP
                     IGAHALTGAFVEGILYGTYRYDDFKTKDPRPVTLKNLQVVPPDDAPVRQHQDALNRIS
                     TIVDATQFARDLGNRPCNDLVPTALARAAREVAAAANLKVTVYDYRKAEALGMHAFCA
                     VAKGSAEEPAFIVIEYNGGKKGDAPVVLVGKGLTFDTGGISLKPAENMDHMKFDMCGA
                     GAVLGAMKAIAELKVPRNVVMLIPSVENMPGGRAYKPGDVLQTLGGPTIEVRNTDAEG
                     RVVLADALAYAKRYKPAGVIDLATLTGACVVALGSECAGLFTPNDMMAARLNAAADAT
                     GDRLWRMPLWDEYREMVKSDVADVRNSTGRAAGAVTAACFLETYADGYPWAHLDIAGP
                     AYNEHNTAYGPKGATGFGVRLLVHLVENWPR"
BASE COUNT          780 a         1736 c         1726 g          944 t
ORIGIN      
        1 catgccgaag ttgccgtaga acgagcgcac cttgccgatc gagtccggac agtcgtggtc
       61 ccaagcgggc ttgccgttct tctcgacgat gcgcgggcgc ggcaggaact tctcgaggaa
      121 gctgcgtacc gcgaccggcc ccgcgccggg gccgccgccg ccgtggggcg tcgagaacgt
      181 cttgtgcagg ttgaactgca gcacgtcggc gcccatgtgc cccggcttgg ccacgcccat
      241 cagcgcgttc atgttggcgc cgtccatgta caccagccca cccgcctcgt gcacgaggcg
      301 ggtaatcttc tcgacatcgc gctcaaagat gcccagcgtg ttgggattcg tcagcatcat
      361 ggccgccgta tccggcccga gcaccgcctt cagcgcctcg acatccacca ggccgttggc
      421 gttcgacttg atctcgatga catgcatgcc ggcaatgcgc accgtggccg gattcgtgcc
      481 gtgggccgaa tcgggaatga tcaccttgtc gcgatgcggc tctccccggt ccatgtggta
      541 ccgccgggtc atgagcatcg ccgtgagttc tccctgcgcg cccgcggcgg gctgcagcgt
      601 gaccgccggc aggccggtga tctcggccag catcgtcttc aagtcatgca gcagcgccag
      661 cgccccctgg ctcacttcct ccggagccag cggatgaatg tccgccagac cgggcatggc
      721 cgcggcgcga tcgttcagct tcgggttgta cttcatcgtg cacgacccga gcggatagaa
      781 atccttgtcg acgtgatggt tcagcacgga gatgttggtg aagtggcgga cgacctcggg
      841 ttcggaaacc tctggcaatt ccggcggctt ttcgcgccgg aattgccgga ggagcttttc
      901 gtcatcgttg ctcggcgccg gcacgtccat cttcggcatc gtgtaaccgc agcgtccgcg
      961 cgacgacatc tcgaagatca gccggttatc cacggctcac ccccgccagc accttgccga
     1021 gtttcgcgat gtcgtcggcg ctccgctttt cggtgaccgc cacgaggagc aggtccttcc
     1081 aggcgggatc gaagcggccg agatcgaggc cgacgagcat ctcgtgcctg aggccctcgg
     1141 ccacgacctg tgcggccggg actggacact tcacgacgaa ttccttcacg tgcggacctg
     1201 gcagcgcgat cgaatacccc ggtatctgcg agaccgtgcg cgctgcgtgt gaggcacgcg
     1261 catgacactg ctcccccagc tcgcgcatcc cattcggccc cagcaggctc atgtacaccg
     1321 tggcgcgatg cgccagcagc gtctggttgg tgcagatgtt cgaggtggcc ttctcgcggc
     1381 ggatgtgctg ctcgcgggtc tggagcgtga gcgtccagca gcgcttgccg tccttgtcga
     1441 cggtttcgcc gacgaggcga cccggcatct ggcggagata cttcgtgtcg gcggcgaaga
     1501 aaccggccac cggtccacca taggccattg gaattcccag gggctggcac tccccgaccg
     1561 cgatgtcggc gccatacgcg ccgggaggtg ccaggatgcc gagcgtgctc ggatcgacca
     1621 ccgcgacgaa cagcgcgccc ttcgcctggg cctttgcgcg gatctcatcc atctgctcca
     1681 gccgcccgaa gaagttcgga tgctgcacga agacggcggc ggtcgattca tccaggttgg
     1741 cgtcgagcca ggcgaggtcg gccacatggt cgggcgccgg atcgaccacc acctggtgcc
     1801 ccgacatctc gacgtaggtc ttgagcacgt cgcggtagtt cggatggaaa cgcccggcga
     1861 tgatcgtctt cggtcgtccg gtgaccgacg cggccagcag cgccgcctcc cccgccgccg
     1921 acgcgccgtc gtacagcgag gcgttggcca ccgcaagtcc ggtgagctcg gaaaccatcg
     1981 tctggaattc gtagatggcg atcagcgtcc cctggctcac ctcggcctgg tagggcgtgt
     2041 aggccgtgta gaactcggaa cgcatggtga cgtggtcgat gacgctcggg atgtggtggt
     2101 cgtagatgcc gccgccgagg aagcaggtgt agcggccggc gccggcgttc cggttgcccc
     2161 acgcggagag atcgcgcacg acctcccatt cggagcgccc gggatggatg tcgaggagtt
     2221 ccttgaggcg cagattctcg gggagcggtg ccaggaggtc ttcgaacttc ttgacgccga
     2281 tggccgcgag catggcggcc tcgtccgccg ccgttccggg aatgtagggg tgggtgccgc
     2341 tcacgaaccg atcagctcct tgtacgccgc ggaggagagg aggccttcga gctcggccgg
     2401 cgcgctcatc ttgaccttga tcatccagcc ggcctcgtac ggcgacttgt tcacgctgtc
     2461 gggcgcgtcg ttgagcgcgg cgttcacgtc gatgatcgtt cccgacaccg gggcgtagag
     2521 gtcgctgacc gtcttgaccg cctcgacggt tccgaaggcg gccatctggc ggacttcgtt
     2581 ccccttgttc ggcagcgtca cgtagacgat gtcccccagc tcaccctgcg cgtagtcggt
     2641 gatgccgatc gtggccacgt ctccctccac gcggatccac tcgtgttcct tcgtgtaatg
     2701 cagcgagtcc ggtatcgtcg tcatgacttg cgtgtccctt cggtgtagaa cggcaggcgc
     2761 tgcacgaccg ctcgggcggt gcgcccccgg atatcgacct gaacctccga gcccggcttg
     2821 gccgagccga tcggcacata ggccatggcg atgctcttct tcagcgacgg cgacatcgtg
     2881 ccgctcgtca cccggccggt caccgtgccg tcggtcagca cgttcatgcc ggcccgcggg
     2941 atcatccccg cctggtcgat ggtaaggccc atcagcttgc gccggagccc ggctgccttc
     3001 tgcgttacca gcgcgtcctt accgatgaag tcgggcttgt cgagcttgac cgtccactgg
     3061 aggcccgcct cgagcggcgt ggtggtgtcg tcgatgtcgt tcccgtagag gcagtagcca
     3121 acctcgagac ggagcgtatc ccgcgcggcc aggccgatgg gggccatgtc gtgcggcttg
     3181 ccggcctcct ggatccgctc ccacatcgtg tcggcgtgcc ggcggtcgaa gaagagctcg
     3241 aaaccgtcct cgccggtgta gccggtccgc gagatcagga ccggaacgcc gtcccacagg
     3301 aattcgcgga agtggtagta gccgagatcg gtgatccagt cgcccgtgaa cggcttgacg
     3361 accgcccccg ccttcggtcc ctggatggcc agcagcgccg tgtcggcgct ccggtcgtgg
     3421 acgatcatgc cttccggcgc atgcgaccgc acccactcca ggtccttctc gatgttggcc
     3481 gcgttcacca ccagcagcac gccgtcttcc cggcggtacg ccaggacgtc gtcgacgatg
     3541 ccgccgtcgg gccggcacat cggggagtag acgatccccc ccggttcctt tgccgagagc
     3601 tggttggtga ggagatggtc ggtgaactcc agcgccccgg ggcccgagaa atgaaactcc
     3661 cccatatggg aaagatcgaa gagccccacg ccggtccgga cggcctcgtg ctccttgagc
     3721 tggccggcga actggagcgg catcaggtaa ccggcaaaat cgacgatctt cccgccgagg
     3781 gcgcggttcc ggtcggtgaa cggggtttcg agggacagat gatcctcgac tttcagtgac
     3841 ggttgtaact gcttgccggt tagccggttg ggaactgtat cacacggctt tcagcggggc
     3901 cagttctcgg ctttcagcgg ggccagttct ccaccaggtg caccaggagc cgcacgccga
     3961 aaccggtggc tcccttcggg ccgtaggccg tgttgtgttc gttataggcc ggaccggcga
     4021 tgtccaggtg ggcccacgga tagccgtcgg cgtacgtctc gaggaagcag gccgccgtaa
     4081 ccgccccggc ggcgcggccg gtggagttcc ggacgtcggc cacgtcgctc ttcaccatct
     4141 cgcgatactc gtcccagagg ggcatgcgcc agaggcggtc gccggtggca tcggccgccg
     4201 cgttgagccg ggcggccatc atgtcattgg gggtgaagag tccggcacat tccgatccga
     4261 gggccaccac gcaggcgccg gtgagcgtgg ccaggtcgat cacgccggcc ggcttgtagc
     4321 gcttcgcgta ggcgagggca tcggccagga ccacgcgccc ctccgcgtcg gtgttgcgca
     4381 cctcgatcgt cgggccgccg agagtctgga gcacgtcgcc cggcttgtaa gcccggccac
     4441 ccggcatgtt ctcgaccgac gggatgagca tgaccacgtt gcgcggcacc ttgagctcgg
     4501 cgatcgcctt catcgcaccc agcaccgcgc ccgccccgca catgtcgaac ttcatgtggt
     4561 ccatgttctc ggccggcttg agggagatgc cccccgtgtc aaacgtgagc cccttcccca
     4621 ccaggaccac cggggcatcg cccttctttc cgccgttgta ctcgatgacg atgaaggccg
     4681 gctcctcggc gctccccttg gccacggcgc agaaggcgtg catgccgagc gcctcggcct
     4741 tcctgtagtc gtacaccgtc accttgagat tggcggcggc ggccacctcg cgggcggccc
     4801 tggccagcgc cgtcggcacg aggtcgttgc aggggcggtt gccgaggtcg cgggcgaatt
     4861 gcgtggcgtc gacgatggtg ctgatgcggt tgagcgcgtc ctggtgctgg cggacgggcg
     4921 cgtcatcggg cggcacgacc tgcaagttct tcagggtgac cggccgggga tctttggtct
     4981 tgaagtcgtc gtaacggtaa gtgccgtaca ggatcccctc gacgaaggct ccggtgaggg
     5041 catgggcccc gatcggcagc ggcacgccgc cggccgtgat cttcttcatc ttctgctcgt
     5101 gggcggcccg ggctacccgc acgcccgcct tccgggcccg gacagcatcg aacttggccg
     5161 cgtcgccgca tcccaccagg agcacg
//
LOCUS       DASVOD010000580         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_143540, whole genome shotgun sequence.
ACCESSION   DASVOD010000580 DASVOD010000000
VERSION     DASVOD010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143540"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..731
                     /gene="lptE"
                     /locus_tag="VF720_12830"
     CDS             222..731
                     /gene="lptE"
                     /locus_tag="VF720_12830"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEX7880291.1"
                     /translation="MTRPRPQLLLLPLAVLLATTLTGCYTVRNSIPDHIKSIAVPVFR
                     NESLQSGVEDEITRKVIATFQSSNALPIADAATANALVDGVVKSYENKVYRIDAQEQA
                     QEYIVIITSNVLVKDRVKNKELWSEEGIRVTATYAVVGPTVRTEAEARSEAVDQLADI
                     IRSRTVEGW"
     gene            728..1807
                     /gene="holA"
                     /locus_tag="VF720_12835"
     CDS             728..1807
                     /gene="holA"
                     /locus_tag="VF720_12835"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEX7880292.1"
                     /translation="MSRPRKGGGDAGIIEARHAMTKGDPAPVVLLAGPERLLAEELIG
                     LAVARFVPPDFQVFNLNRYRGGQDDAETVLNTAATLPMFCDRRVVVVMDADEFSRAEN
                     DRLAAYIASPSPSTLLILVASETGEKLPAVLKKVGERYTLWRPFAKDGIAWVIGRARE
                     LGHELPPPVAEELYALLSGESGDGRASLGDLDIEIQKICLSAGARKRIGTEDLRVVSR
                     HAESRVLYQIESAVAERQLAPALAALDAALLFPRDNGPIRIVAMLGERFRKMLVAKDR
                     MTAGYGAAAVLAGMWFGGPAGSAPFLRSVNLFTRNELSEAVRALARLDRSLKTGQADP
                     DRLHLEAAIASICGRVGAGAGVRVG"
BASE COUNT          311 a          675 c          663 g          315 t
ORIGIN      
        1 cttctttggt atccggttcg ctggctggaa gccagtcgcc ggtcgtgccg gacgccggac
       61 gtgccggtgg acctggtttg cccacaggtc cgccaactag gttcaagccg agttgcagag
      121 cgcgccgcgt gagcgggcca actatgttca gaccgagttg cgcccgcttc cagcttgccc
      181 gcccttgggc gtaaccgggc cccctgttaa catttggccc catgactcga ccgcggcccc
      241 aacttctgct cctcccgctt gcggtgctcc tggccacgac cctcaccggc tgttacaccg
      301 tccggaattc catccccgat cacatcaaga gcatcgcggt gccggtcttc cggaacgagt
      361 cgctgcagtc gggcgtcgag gatgagatca cccgcaaggt gatcgccacc ttccagtcga
      421 gcaacgcgct gccgatcgcg gatgcggcga cggccaacgc gctggtggac ggggtcgtga
      481 agagctacga gaacaaggtg taccggatcg acgcgcagga gcaggcgcag gagtacatcg
      541 tcatcatcac gagcaatgta ctggtgaagg accgggtgaa gaacaaggag ctctggtccg
      601 aggaggggat ccgggtcacg gccacctacg cggtggtggg gccgacggtc cgcacggagg
      661 ccgaggcccg ctcggaagcg gtcgaccagc tagccgacat catccgctcc cgcaccgtgg
      721 aggggtggtg agccggccgc gcaagggggg cggggacgcc ggcatcatcg aggcccgcca
      781 tgccatgacc aagggggatc cagccccggt cgtcctgctg gccgggcccg aacgtctcct
      841 cgccgaggag ctcatcgggc tggccgtggc gcgcttcgtg ccgcctgact tccaggtgtt
      901 caacctgaat cgctatcggg gtggccagga tgacgccgaa actgtactga acacagcggc
      961 cacgctcccg atgttctgcg atcgccgggt ggtggtcgtc atggatgccg acgagttctc
     1021 gcgcgccgag aacgaccgcc tcgccgccta catcgcgagc ccctcgccca gcacgctgct
     1081 gatcctcgtg gcatccgaaa cgggggagaa gctgccggcc gtcctcaaga aggtggggga
     1141 gcgatacacc ctctggcgtc cgttcgccaa ggacggcatt gcctgggtga tcggccgggc
     1201 ccgggagctg gggcacgaac tgccgcctcc ggtggccgag gaactctacg cgctgttgtc
     1261 cggcgaatcg ggtgacggcc gggccagcct cggcgatctc gacatcgaaa tccagaagat
     1321 ctgcctctcc gctggcgccc ggaagcgaat cggcaccgag gacctgcgcg tcgtcagtcg
     1381 gcacgccgag tcgcgcgtgc tgtaccagat cgaatcggcc gttgccgaac gacagctcgc
     1441 ccccgccctc gcggcgctcg acgcggcact cctcttcccg cgcgacaacg gccccatccg
     1501 catcgtggcg atgctgggcg agcgcttccg caagatgctg gtggccaagg accgcatgac
     1561 cgccggttac ggtgccgccg cggtgctcgc cggcatgtgg ttcggtggcc cggcggggtc
     1621 agcgcccttc ctccgctcgg tgaacctctt cacgcgaaac gagctctccg aagccgtccg
     1681 cgcgctggcc cgactcgatc gttccctcaa gacgggccag gccgacccgg accggctgca
     1741 cctggaggcc gcgatcgcct cgatctgcgg ccgggtgggg gcgggggcgg gagtccgggt
     1801 gggctgagcg tccctccacg atatagaatc gatttcaggt ctccgcgtcg agcgtcgatc
     1861 cgccggggct ccccccaggg cagggccagt gaggtcgcga tgagtccgat tgcccacact
     1921 ccgatccatg ccaggcgccg gccagttccc tccctcggac gcgc
//
LOCUS       DASVOD010000581         2693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_401942, whole genome shotgun sequence.
ACCESSION   DASVOD010000581 DASVOD010000000
VERSION     DASVOD010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401942"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="VF720_12840"
     CDS             complement(<1..2231)
                     /locus_tag="VF720_12840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7880293.1"
                     /translation="MTDSDHWKRIQDLFEELLDLDEADCERRIESVDNEVAAEVRSLL
                     RAHRAASGFLDRTDPSGPSGPTPVGSWGTRLESAFAPGMRLGPYRLEEEIGHGGMGIV
                     FRATRDDENFTKEVAVKLIDPGRRSDDILRRFRAERQILAMLDHPHIARLVDGGTAPD
                     GSPYLVMDFVSGRPLLEYCDHHRLGIDQRLALFLAVCDAVQFAHQRLVVHRDLKSDNI
                     LVTDDGTPRLLDFGIARLLTPEGDTGTLTLPMQRMLTPDYASPEQVRGEPVTVAGDVY
                     SLGVILYELLSGSRPHKFKTRSPEEILRVITQEEPVAPSAAMARSATQLAAEQRGDTT
                     RRLRKRIAGDLDDVVLKCLEKDPARRYGTAEQLAADIRRHLAGEPVSARGHTTAYLIS
                     RFVRRHRVAVTSGSLVLVALLIGLGATMWQAREAKRERDLAERRFADVRELAHAVVFP
                     IHDAIINLPGSTKAREILVEHALRYLDGLSRDAGADDPKLLLELGDAYGKIGDVQGRP
                     MWPNLGQSEAALASYDRALALLDRACAAMPDSVIPINDYLVTMQRRADLLNTMGRPDE
                     ALAQSVAAGGRAREGARRHPTSVLLHKCVVVSYARLSDMYLARGDTAAALAAGDTLSV
                     SADALVRLAPDDPDNRRSQMISAAKGADLLAHQGHRAAADSSYRVAVRLASEAAREFP
                     DNTDAMRDLSIVYGMFGFFQSEAGDIDSALATYEKGMQIVADLAERDPDNVNNQADVA
                     LGDL"
     gene            complement(2315..>2693)
                     /locus_tag="VF720_12845"
     CDS             complement(2315..>2693)
                     /locus_tag="VF720_12845"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7880294.1"
                     /translation="ADTDVLIDFLHGVEPGAGRIAAELETGQLRTTVVSRVELLAGAR
                     SPRQETVIRELLAAVPSLPLDDAAAEHAGRVRRELDAAGSAIGLGDCLIAGIALANQA
                     ILLTRNRQHFEPVSGLSLGTLST"
BASE COUNT          389 a          881 c          997 g          426 t
ORIGIN      
        1 aggtcgccca gcgccacgtc ggcctggttg ttgacgttgt cgggatcgcg ttccgccagg
       61 tctgcgacga tctgcatccc cttctcgtat gtcgcgagcg ccgagtcgat gtcacccgcc
      121 tcggactgga agaagccgaa catcccgtag acgatcgaga ggtcgcgcat cgcatcggtg
      181 ttgtcgggaa attcgcgggc ggcttcgctg gccagtcgga cggccacgcg gtacgacgag
      241 tcggcggcgg cgcgatgccc ctggtgggcc aggaggtcgg cccccttggc ggcgctgatc
      301 atctgcgagc ggcggttgtc ggggtcatcg ggggcgagcc gcacgagcgc gtcggcggag
      361 acggacagtg tgtcgccggc ggccagggcc gccgccgtgt caccgcgggc caggtacatg
      421 tcgctgagcc gtgcgtacga cacgaccacg cacttgtgca gcaggacgct cgtcggatgg
      481 cgtcgggcac cttcgcgggc ccggccgccg gctgccacgc tctgagccag cgcctcgtcg
      541 gggcgcccca tcgtgttgag caggtcggcg cggcgctgca tcgtcaccag gtagtcgttg
      601 atcggaatga ccgaatcggg catcgccgcg caggcgcgat cgagcagggc cagcgcgcga
      661 tcgtagctgg ccagggcggc ttcgctctgg ccaaggttgg gccacatcgg gcgcccctgc
      721 acgtcaccga tcttgccgta ggcgtcgccc aactcgagga ggagcttcgg gtcgtcggcc
      781 ccggcatcgc gcgagagtcc gtcgagatag cgcagcgcgt gctccaccag gatctcgcgc
      841 gccttcgtcg agccgggaag gttgatgatg gcgtcgtgga tcgggaagac gacggcgtgt
      901 gccagttcgc gcacatccgc gaaccgccgc tcggccaggt cgcgctctcg tttcgcctcc
      961 cgggcctgcc acatcgtggc gccgagcccg atgaggagcg ccacgagaac gagtgagccc
     1021 gacgtgaccg ccacccggtg ccgccgcacg aagcgcgaga tgagataggc cgtggtgtgt
     1081 ccgcgggccg ataccggctc gccggccagg tggcgccgga tgtcggcggc aagctgttcc
     1141 gcggtgccgt atcggcgggc ggggtctttc tcgaggcact tcaggaccac gtcgtcgaga
     1201 tcgccggcga tgcgtttccg cagccgccgc gtggtgtcgc cccgctgctc ggcggcgagc
     1261 tgtgtggccg atcgggccat cgccgcgctc ggggccaccg gttcttcctg cgtgatgacg
     1321 cgcaggatct cctcgggcga tcgggtcttg aacttgtgcg gcctcgagcc cgacaggagc
     1381 tcgtacagga tgacgcccag cgagtagaca tcacccgcca ccgtgaccgg ctcgccacgc
     1441 acctgctcgg ggctggcgta gtcgggcgtg agcatccgct gcatcggcag ggtgagcgtg
     1501 ccggtgtcac cctcgggcgt gaggagccgg gcaatgccga agtcgaggag gcgcggcgtg
     1561 ccgtcgtcgg tcaccaggat gttgtccgat ttcaggtcgc ggtggacgac caggcgctgg
     1621 tgcgcgaact gcacggcgtc gcacacggcc aggaagaggg ccaggcgctg gtcgatgccc
     1681 agtcggtggt gatcgcagta ctcgaggagc ggccggcccg acacgaaatc catgaccagg
     1741 taggggctgc cgtcgggagc ggtgccgccg tccaccagcc gggcgatgtg cgggtggtcg
     1801 agcatcgcca ggatctgccg ctcggcccgg aagcgccgga ggatgtcgtc cgagcggcgg
     1861 ccgggatcga tcagcttgac ggccacttcc ttggtgaaat tctcgtcgtc gcgcgtggcg
     1921 cggaagacga tccccatgcc gccgtgcccg atctcttcct cgaggcgata ggggccgagg
     1981 cgcatgccgg gggcgaaggc cgattccagg cgcgtccccc agctgccgac gggagtgggg
     2041 ccggagggcc ccgacgggtc ggtccggtcg aggaagccgc tggcggcgcg gtgagcccgc
     2101 aggagcgagc ggacttcggc ggccacctcg ttgtccacgg actcgatgcg gcgctcgcag
     2161 tcggcctcgt cgaggtccag gagttcctcg aagagatcct ggatgcgttt ccagtgatcg
     2221 gaatcggtca tgctgcggcc tcaaatgatc cggccgcgga ccgcggcggg tggaggcatg
     2281 ataggcgatc ggcggggcct gtcgcgccgt cggatcaggt gctcaatgtc cccagtgaca
     2341 ggccgctcac gggctcgaaa tgctgccggt tcctcgtgag caggatggcc tggtttgcca
     2401 gcgcgatgcc ggcgatcagg caatcgccca gtccgatggc actcccggcc gcatcgagct
     2461 cacgtcggac gcgcccggca tgctcggcag cggcatcatc gagggggagg ctgggaacgg
     2521 cggccagcaa ctcccggata accgtttcct ggcggggcga tcgggctcct gcgagcagct
     2581 cgacccggct caccaccgtg gtgcggagct gtcctgtctc aagctcggcg gcgatccggc
     2641 cggcaccggg ctcgacccca tgcaggaaat cgatcaggac atcggtgtcc gcc
//
LOCUS       DASVOD010000582         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_924243, whole genome shotgun sequence.
ACCESSION   DASVOD010000582 DASVOD010000000
VERSION     DASVOD010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924243"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VF720_12850"
     CDS             <1..570
                     /locus_tag="VF720_12850"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEX7880295.1"
                     /translation="YRVVSELLGAGRETASSYEIARLSGTTSSQLRKDLSNFGNFGKR
                     GTGYRVAALHEELKAILGLNRRWRVAVVGAGNLAHALAEYREFRRQGFEIAAVFDIDP
                     ARIGQEWDGVAVRHFDEFLAAARTEHFEMGVITTPAAAAQEVADRMVEAGIQGILNFA
                     SVKLTVPEHVTLRNVDMAIELESLSFALK"
     gene            complement(573..1367)
                     /locus_tag="VF720_12855"
     CDS             complement(573..1367)
                     /locus_tag="VF720_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX7880296.1"
                     /translation="MRVGFVQYEPVFGEVAANLAAIETAVEAAGRADLMVLPELATTG
                     YNFATVEEAEALSEPVPGPSTERLAALARRLETWLVVGIAEAAGGKRYNSSVLIGPDG
                     SVGTYRKLHLYDREKLFFVPGDLGLPVFRLPVPGDPVIGMMICFDWRFPEAARGLALA
                     GADIIAHPSNLVLTLCQAAMITRCLENRVFAVTANRTGVEDRGGVRIGFTGGSQIVDP
                     NGVVLAAADPANSGSAVVTIDLAEARNKSINSRNDLWGDRRVGEYG"
     gene            1479..1691
                     /locus_tag="VF720_12860"
     CDS             1479..1691
                     /locus_tag="VF720_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013898223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEX7880297.1"
                     /translation="MSDDTPKRLTRSRRDRMILGVAGGFAHYFNMDPTIFRLLFVGAV
                     VFTALFPGILFYIVCAIIIPEEPVSA"
BASE COUNT          291 a          695 c          533 g          291 t
ORIGIN      
        1 taccgcgtcg tgtccgaact gctgggcgcc ggccgggaga cggccagctc ctacgaaatc
       61 gcgcgcctct ccggcaccac ctcgagccag ctgcggaagg acctctccaa cttcggcaac
      121 ttcggcaagc gcggcaccgg ctaccgcgtg gccgccctcc acgaggagct caaggccatc
      181 ctcggactca accgccgctg gcgcgtggcc gtggtgggcg ccggtaacct ggcccacgcc
      241 ctcgccgaat accgcgagtt ccgtcgccag gggttcgaga tcgccgccgt gttcgacatc
      301 gatcccgccc gcatcggcca ggagtgggac ggcgtggccg tccgccactt cgacgaattc
      361 ctcgccgccg cccgcaccga acacttcgaa atgggcgtca tcaccacccc cgccgccgcc
      421 gcccaggaag tggccgaccg catggtcgaa gccggcatcc agggaatcct caactttgcc
      481 tcggtcaagc tgacggtgcc ggagcacgtg accttgcgca acgtggacat ggcgatcgag
      541 ctggagagct tgagtttcgc gctgaagtag tcctacccgt actcccccac ccgtcgatcc
      601 ccccaaagat cattccgcga attgatcgac ttgttccgcg cctccgccag gtcgatcgtg
      661 acgaccgcgg agccggagtt cgcgggatcc gccgccgcca gcacgactcc attcggatcg
      721 acgatctggc tcccgccggt gaatccgatg cgcacgcctc ctcggtcttc cacgccggtc
      781 cggttggccg tcaccgcgaa gacccggttc tcgaggcacc gcgtgatcat cgccgcctgg
      841 caaagcgtga gcaccaggtt cgacgggtgg gcaatgatgt ccgcgcccgc cagggccagc
      901 ccgcgcgccg cctccgggaa ccgccaatcg aaacagatca tcattccgat gaccgggtcg
      961 ccgggcacgg ggagccggaa cacgggaagg ccgagatcgc ccggcacgaa gaacagcttc
     1021 tcccgatcgt agagatgcag tttccggtag gtgcccaccg agccatccgg cccgatcagc
     1081 acggagctgt tgtagcgctt cccaccggcc gcctcggcga tccccaccac cagccacgtc
     1141 tcgagccgcc tcgccagcgc cgcgagccgc tcggtggacg gccccgggac gggctcggaa
     1201 agcgcctccg cctcctccac cgtggcaaag ttgtagccgg tggtggccag ctccgggagc
     1261 accatcaggt cggcccggcc ggccgcctcc accgcggttt cgatggccgc gaggttggcc
     1321 gcaacttcgc cgaagaccgg ctcgtactgc acgaacccaa cgcgcatgga cctcccccgg
     1381 caatcgacgg cccgccctcc tcgtgacggc cgccatcgaa cgccatctgt agtacattcc
     1441 ccaaaccggc cccgccggtc accggaggac ccgaaacgat gtccgacgac accccgaagc
     1501 gcctcacccg ctctcgccgc gatcgcatga tcctcggcgt ggccggcggc tttgcacact
     1561 acttcaacat ggatcccacc atcttccgac tcctgttcgt cggcgcggtc gtcttcaccg
     1621 cactgttccc cgggatcctg ttctatatcg tctgcgccat catcatcccc gaggagccgg
     1681 tgtccgccta gtgggcgcct tccccatcgt caccggaatc ctgtcggtgc tcatcggcac
     1741 cctggtcggc ctctcgctcg gcctcgtggg cgcgggcggc tccatcctca ctgttccggc
     1801 cctgatctac
//
LOCUS       DASVOD010000583         4433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_714919, whole genome shotgun sequence.
ACCESSION   DASVOD010000583 DASVOD010000000
VERSION     DASVOD010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714919"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VF720_12865"
     CDS             complement(<1..993)
                     /locus_tag="VF720_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880298.1"
                     /translation="MSDDFLGGLRGRPTPEFKAQLRERLRRQEGSDFSMSDDDLPVRA
                     GRRSWPLVAAGVLAAAVVALIASPAVRTSAGAFLELFRVRRFAAVPVDPARLERLESS
                     TIDLKGLLAENVETIQEPAPPQFFTSVASAASTAGFPIRVPAIIPSGMAPDTVTVRGG
                     GEARFTARTTKLREVLDALGLTDVTIPPGLDGAQVGLKTSTSVMVRYQSEKRRIGFYQ
                     ARSPEVSLPSGVDLAEVGEIGLRIAGLERTDAQRFARSIDWHSTMLVPMPINAGSFRE
                     VDVRGNKGLMLTMSGEPGPDGSKGREGSMLMWADGDMVYALRGNVESGDLLEMAT"
     gene            complement(983..1525)
                     /locus_tag="VF720_12870"
     CDS             complement(983..1525)
                     /locus_tag="VF720_12870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7880299.1"
                     /translation="MIQGKFFPWQESKEADWDALYEEHLPQIYNFFRYRLRNPADAED
                     LTAETFEKAWRARDRYRRDRGAFGGWLFTIARNLAVDHYRARREMVPLEAVSEISGGA
                     TPEELSVRRSDAERLTRLLGQLPERERELIAWKFGAELSNGEIARLSGLTQSNVGTLL
                     HRTIRQLREEWIQEDKRHER"
     gene            complement(1680..3164)
                     /locus_tag="VF720_12875"
     CDS             complement(1680..3164)
                     /locus_tag="VF720_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX7880300.1"
                     /translation="MAAKKPNILILWGDDIGYWNISTYNLGQMGYRTPNIDRIAKEGA
                     VFTDWYGQQSCTAGRACFITGQSGFRTGMLKVGLPGAKEGLQARDVTIAELLKAQGYM
                     TAQFGKNHLGDADETLPTAHGFDEFFGSLYHLNAEEEFENEDYFKDPALIEKYKTRGL
                     LHCWANPDGTQKIESKGPLGKKRMETVDEEVTALTLDYLERAKKADKPFFLWWNSTRM
                     HINTHLKPESRGKTGLGVYPDGMVEHDGHVGQILDKLKELGLDDNTIVMYSTDNGAES
                     FSWPDDGTTPFRGEKNENWEGGYRVPCAIRWPGTIEPGTVNNDLFSHEDMLPTLLAAA
                     GVPDVKEQLLKGMKVGDKSFKVHLDGYNITDRLAGKGENPRNEFFYWNDDGSLVGLRF
                     NNWKIVFQEQRSEGLDVWQDPFTPLRLPKIFNLRSDPFETADEIAMGYAKWRIDRLYL
                     LVPAQEYIAKYIATFKDFPPSQAVGSFSLDQVLDKLTAASTSGR"
     gene            complement(3275..3943)
                     /locus_tag="VF720_12880"
     CDS             complement(3275..3943)
                     /locus_tag="VF720_12880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX7880301.1"
                     /translation="MPRSRPGAVPATVLALLFAALLSVPAAHAIEPATELRLPLDSYP
                     IDDNCGPWGWFNPNFLRCGMPGKHVADDACAPAGTMVYAVGPGRVMHGSQVGNCNTNW
                     GWLTVVEHERPDGSKFCSIYGHCRPLAGILPGTEVEKGQAIAIVEFPCGAQHIHFGIA
                     IGGFDVDLGYYPSWLLGYLPDGITCEGQPTPFPGRYVEPVQFVLDTVPVEAVTWSGVK
                     ARER"
BASE COUNT          708 a         1429 c         1506 g          790 t
ORIGIN      
        1 ggtggccatc tcgaggagat cgccgctctc cacgttgccg cgcagagcat agaccatgtc
       61 gccatcggcc cacatgagca tcgacccctc gcggcccttg ctgccgtcgg gtccgggctc
      121 gccgctcatc gtgagcatga gccccttgtt gccgcggaca tccacttcgc ggaacgaacc
      181 cgcgttgatc ggcatcggaa cgagcatcgt actgtgccag tcgatggagc gggcgaagcg
      241 ctgcgcgtcg gtacgctcga ggccggcgat gcggaggccg atttcaccga cctcggcgag
      301 atccacaccc gatggcaacg acacttccgg gctccgggcc tggtagaagc cgatgcggcg
      361 tttctcgctc tggtagcgaa ccatgaccga ggtcgatgtc ttcaggccca cctgggcgcc
      421 gtcgagtccc ggcggaatcg tcacgtcggt caggccgagc gcgtcgagca cttcgcggag
      481 tttcgtggtg cgagcggtga agcgggcctc gccgccgccg cgcaccgtga ccgtgtcggg
      541 cgccatgccc gatggaatga tggccggaac ccggatcgga aagccggccg tcgatgcggc
      601 cgacgccacg ctcgtgaaga actggggcgg ggccggttcc tggatcgttt cgacattctc
      661 ggccagcagc cccttcaggt cgatcgtgct gctctccagc cgctcgaggc gggccggatc
      721 gacgggaacg gccgcgaagc gacgcacccg gaacaactcg aggaacgcac cggccgaggt
      781 gcgcaccgcg ggggaggcga tgagcgccac gaccgccgcc gcgagcaccc ccgcggccac
      841 gaggggccag gaacgccgtc ccgcgcgcac cggcagatca tcatcactca tcgaaaaatc
      901 gctcccttcc tggcgacgca accgctcccg cagctgcgcc ttgaactccg gcgtgggccg
      961 gccgcgcagg ccgcccagga aatcatcgct catgacgctt gtcctcctgg atccactcct
     1021 cccgcagctg cctgatcgtg cgatggagga gggtccccac attcgattgg gtgagcccgg
     1081 agaggcgggc gatctcaccg ttcgacagct ccgccccgaa cttccaggcg atgagctccc
     1141 gctcccgttc gggaagttgc ccgaggaggc gcgtcagccg ttccgcgtcg gatcgccgca
     1201 ccgagagttc ctcgggcgtg gcgccgccgg agatttcgct caccgcctcc agcggcacca
     1261 tctcgcgccg cgcgcggtaa tgatcgacgg ccaggttccg ggcgatcgtg aagagccatc
     1321 ccccgaacgc gccccgatcc cgccggtaac gatcccgcgc ccgccatgcc ttctcgaacg
     1381 tctcggcggt caagtcctcg gcatccgccg ggtttcgcag gcgatagcgg aagaagttgt
     1441 agatctgcgg caggtgctcc tcgtagaggg catcccagtc cgcttccttc gactcctgcc
     1501 acgggaagaa tttgccttga atcacgttat cgcgagtggc cacggggagg attacgggtg
     1561 aaggggcaac gtatcacagg atttcagcgg ggagggcgaa cccgcgtcgt aagacaaagg
     1621 ccggcagcca catcgggctg ccggcctcgg aagatcggat gtgtgggatc cgggtacggc
     1681 taccggccgc tggtgctggc cgccgtgagc ttgtcgagca cctggtcgag cgagaagctg
     1741 ccaacggcct ggctgggcgg aaaatccttg aaggtggcga tgtacttcgc gatgtactcc
     1801 tgcgccggca caagcaggta gaggcggtcg atgcgccact tcgcgtagcc catggcgatc
     1861 tcatcggccg tctcgaacgg atcggaacgg aggttgaaga tcttggggag ccgcagcggc
     1921 gtgaacgggt cctgccagac atcgagcccc tcgctccgtt gctcctggaa gacgatcttc
     1981 cagttgttga agcgcagccc caccagcgag ccgtcgtcgt tccagtagaa gaattcgttg
     2041 cgcgggttct cccccttgcc cgccagtcgg tcggtaatgt tgtagccgtc caggtgcacc
     2101 ttgaacgatt tgtcgccgac cttcatcccc ttgaggagtt gctccttcac atccggcacg
     2161 ccggcggcgg ccagcagggt ggggagcatg tcctcgtgcg agaagaggtc gttgttgacc
     2221 gtgccgggtt cgatcgtccc cggccagcgg atggcgcagg gaacgcggta gccaccctcc
     2281 cagttctcgt tcttctcgcc gcggaacggt gtcgtgccgt cgtccggcca gctgaacgac
     2341 tcggcgccgt tgtcggtgga gtacatcacg atggtgttgt cgtcgaggcc gagctccttg
     2401 agcttgtcga ggatctgccc cacgtggccg tcgtgctcga ccatgccgtc ggggtacacg
     2461 cccagcccgg tcttgccacg gctctccggc ttcaggtggg tgttgatatg catgcgggtg
     2521 gagttccacc agaggaagaa gggcttgtcg gccttcttgg cccgctcgag atagtcgagc
     2581 gtaagggccg tgacttcctc gtcgaccgtc tccatccgct tcttgccgag cggccccttg
     2641 ctctcgatct tctgcgtgcc gtcgggattg gcccagcagt ggagcaggcc gcgcgtcttg
     2701 tacttctcga tcagcgccgg gtccttgaag tagtcctcgt tctcgaattc ctcctcggcg
     2761 ttgaggtggt agagcgagcc gaagaactcg tcgaacccgt gcgcggtcgg cagcgtctcg
     2821 tccgcgtcgc ccaggtggtt cttgccgaac tgcgcggtca tgtaaccctg ggccttgagc
     2881 agttcggcga tcgtcacgtc ccgcgcctgc aagccttcct tcgcgccggg aagccccact
     2941 ttcaacatcc cggtccggaa tcccgactgc ccggtgatga agcaggcgcg accggccgtg
     3001 cagctctgct ggccgtacca gtcggtgaag acagcccctt ccttcgctat ccggtcgatg
     3061 ttcggagtcc ggtaccccat ctggccaagg ttgtaggtgc tgatgttcca gtacccgatg
     3121 tcgtcgcccc agaggatcag gatgttcggt ttcttcgcgg ccatgaatct tccttttgcg
     3181 ttgtgtggtg aaaccatggg gatggcggaa taaggggcgg agtgtgtgcc cgggccggtc
     3241 gaaacgcaac gccccggtga ctacggggcg tttcctaccg ctctcttgcc ttcaccccgc
     3301 tccaggtgac cgcctccacg ggcaccgtat cgagcacgaa ctgcaccggc tcgacgtagc
     3361 ggccggggaa gggggtcggc tgcccctcgc aggtgatccc atccggcaga tagccgagga
     3421 gccaggaggg gtagtagccg aggtccacgt cgaagccgcc gatggcgatg ccgaagtgga
     3481 tgtgctgggc tccgcagggg aactcgacga tggcgatggc ctgccccttc tcgacttccg
     3541 tgccggggag gatgccggcc agcggccggc agtggccgta gatcgagcag aacttgctgc
     3601 cgtcggggcg ctcgtgctcg acgacggtga gccatcccca gttggtgttg cagttgccga
     3661 cctgtgagcc gtgcatgacc cggccggggc cgaccgcgta caccatggtg ccggcggggg
     3721 cgcaggcgtc atcggccacg tgcttgccgg gcatgccgca gcgcaggaag ttgggattga
     3781 accaccccca cgggccgcag ttgtcgtcga tggggtagct gtcgagcggg agtcggagtt
     3841 cggtggccgg ctcgatggcg tgggcggccg ggacggatag gagggcggcg aaaagcaggg
     3901 caaggacggt cgccgggact gcgccggggc gggaacgggg catctggggt ctccgggaaa
     3961 tcggtgtcgc agccggttcc gggatggtga gggggaagac gatcgtaacc ggcggatcgt
     4021 tcagttgcca gtgcacttcc ggcgtggagt cctgaaaagg tcagtgggcc gaaggcggca
     4081 cccggcaacc cgaggtgatg aagccgatca cggcgcaggc acccgggtgc tcggtgtttc
     4141 ccggaagaca gggtgagtcc gactcgacct ccatggactt ggcggacggg ttacacagtg
     4201 gcccggttcc cccggatccg gaacccccgg ggccatcgcg gccgacgacc agttctcctc
     4261 gttcaatgga acgagggcat atcgcaactc gtaaccgctc gcccggccca ccgcgaagtc
     4321 atcgcctgac gccgtccacg ccaggtcgac gctgtttggc gtcgctccaa gcacgcggag
     4381 atcgttcacc gccgcgggcg gggtgacgtc ggctgccatc gtgtcgggtg agg
//
LOCUS       DASVOD010000584         1961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_402216, whole genome shotgun sequence.
ACCESSION   DASVOD010000584 DASVOD010000000
VERSION     DASVOD010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402216"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF720_12885"
     CDS             <1..835
                     /locus_tag="VF720_12885"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HEX7880302.1"
                     /translation="QQDPLGLAHAVLVSEPFLKDDTFVMFLGDNLIKDGITGLVDEFR
                     REKPNSQILLAEVKNPQEFGVAELEGDKIVRLEEKPKEPRSNLALVGVYMFDPTVFEA
                     VHAIKPSKRNELEITDAIQYLIDKKYDVRSHVIGGWWKDTGKLEDMLEANRIILSGLH
                     KKIEGTLAADTRVEGEVVVGAGSKVDASILRGPIVIGRNCVIRSAYVGPFTSIADDCT
                     VIDSEVEHSIILEGSSITGLRRRMESSLIGKGVTVTRQPDRLPQAYRLMVGDSSQIEV
                     F"
     gene            860..1726
                     /gene="rfbD"
                     /locus_tag="VF720_12890"
     CDS             860..1726
                     /gene="rfbD"
                     /locus_tag="VF720_12890"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEX7880303.1"
                     /translation="MRILVTGHTGLLGSELVPVLAAAGHEVHGCSLPDRDITDHDSAR
                     KAVSEARAELVLHAAAWTAVDECEKDPERAYRVNALGTRNMVLAAREANASVAILSTD
                     YVFDGTKKGAYVEFDAANPLTAYGRSKWAGEQYARYLHDRFYIIRSAWLFGAGGPCFP
                     ETILRLLREKGSVDVVTDQVGNPTWTRDLGVAIERIVTSGLYGTYHAVNTGPASWFDF
                     ARAVAVAFGHPAEAVKPTTSDRFVRPAPRPANSALENFVLENSLHHRMRPWQETLSEY
                     VASTGNRFMESA"
     gene            1759..>1961
                     /locus_tag="VF720_12895"
     CDS             1759..>1961
                     /locus_tag="VF720_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX7880304.1"
                     /translation="MRPEDQDRIAAAFRESAALFTTMAEAMAADVARAADLLHDSLAS
                     GGTLLCCGNGGSAADAQHITAELG"
BASE COUNT          345 a          681 c          633 g          302 t
ORIGIN      
        1 ccagcaggac cccttgggcc tcgctcatgc cgtcctcgtc tccgagccgt tcctgaagga
       61 cgacacgttc gtcatgttcc tcggcgacaa cctgatcaag gacggcatca ccggcctcgt
      121 cgacgaattc cggcgcgaaa aaccgaacag ccagatcctc ctggccgagg tcaagaatcc
      181 gcaggagttc ggcgtggccg agctcgaggg ggacaagatc gtccgcctgg aagagaagcc
      241 gaaggagccc cgctcgaacc tggcgctggt cggcgtctac atgttcgacc ccaccgtctt
      301 cgaggcggtg cacgccatca agccgtcgaa gcggaacgag ctcgagatca ccgacgccat
      361 ccagtacctg atcgacaaga agtacgacgt ccgctcccat gtcatcggcg gctggtggaa
      421 ggacaccggc aagctcgagg acatgctcga ggccaaccgg atcatcctgt cgggcctcca
      481 caagaagatc gaggggacgc tggcggccga cacccgcgtg gaaggagagg tggtggtcgg
      541 ggccggctcg aaggtcgatg cctccatcct ccgcggcccg atcgtgatcg gcaggaactg
      601 cgtgatcagg tcggcctacg tcggtccctt cacctcgatc gccgacgatt gcacggtcat
      661 cgacagcgag gtcgagcact cgatcatcct cgagggctcc tcgatcaccg gcctgcgccg
      721 ccggatggaa tcaagcctga tcgggaaggg cgtcaccgtc acacgccagc cggaccgcct
      781 tccccaggcc taccgcctca tggtcggcga ttccagccag atagaggtgt tctagccggt
      841 accggccggc ggaacgatca tgcgcatcct cgttaccgga cacacggggc tcctgggaag
      901 cgaactcgtg ccggtcctcg cggccgccgg ccacgaggtg cacggctgct ccctccccga
      961 ccgcgacatc accgatcacg actcggcccg gaaggccgtg tcggaggcca gggccgagtt
     1021 agtcctgcac gcggcggcct ggacggccgt ggatgagtgc gagaaggacc cggagagggc
     1081 ctaccgcgtg aacgcactcg gcacgcgcaa catggtgctc gccgcccggg aggccaacgc
     1141 gtccgtggcc atcctctcga ccgattacgt tttcgacggc acgaagaaag gggcgtacgt
     1201 ggagttcgac gccgccaatc ccctcaccgc ctacggccgc tcgaagtggg ccggtgagca
     1261 gtacgcccgc tacctgcacg accgcttcta catcatccgg agcgcgtggc tcttcggcgc
     1321 gggcgggccc tgcttccccg agaccatcct ccgcctcctg cgcgagaagg ggagcgtgga
     1381 cgtggtcacc gatcaggtcg gtaaccccac ctggacgcgc gacctgggcg tggccatcga
     1441 gcggatcgtc acgtcggggc tctacggcac ctaccacgcg gtgaataccg gcccggcctc
     1501 ctggttcgat tttgcccggg ccgtggcggt ggccttcgga catccggccg aagccgtgaa
     1561 gcccactacc agcgatcggt tcgtgcgccc cgcgccacgt ccggccaact cggccctgga
     1621 gaatttcgtg ctcgaaaatt cccttcatca ccggatgcgc ccctggcagg aaacgctgtc
     1681 ggagtacgtg gcctccaccg gcaatcgatt catggagtcg gcttgagcgg cgagaggcgt
     1741 ccggctgaat cccccgggat gagaccggaa gatcaggacc ggatcgccgc cgccttccgg
     1801 gagtcggccg cgctcttcac gaccatggcg gaagcgatgg ccgccgacgt ggcccgggcc
     1861 gccgacctcc tccacgactc gctggcctcg ggaggcacgc tcctctgctg cggcaacggc
     1921 ggttcggcgg ccgatgccca gcacatcacg gccgagctgg g
//
LOCUS       DASVOD010000585         7379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_715074, whole genome shotgun sequence.
ACCESSION   DASVOD010000585 DASVOD010000000
VERSION     DASVOD010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715074"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /gene="rimO"
                     /locus_tag="VF720_12900"
     CDS             <1..1244
                     /gene="rimO"
                     /locus_tag="VF720_12900"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX7880305.1"
                     /translation="AFLQAAVEEGIDTIVDVARLKAANLKGLVVTGCMSQRYSGQLLT
                     EIPEIDAIVGTGAVDEIVEVCRNVLDEKGVRTELVGDPNRSYLDPAVRVLSTPRWSPY
                     LQVSQGCSNTCTFCILPKLRGDGRSRSLEDVVGEARRLVQSGAREIVLIGQDTTAWGL
                     DTEGRAFLADCYRRLDELEGDGLKWVRVMYTNPLFWDEALVESFGASRTACSYIDMPI
                     QHSETRVLERMGRGYTREHCAWLVRRLREVRPDVALRTSIIVGFPGETEAEFEATLAF
                     LEEARFDRLVAFPYFPEEGTAAFKLDGRVPDDVQQRRVATLLDLQGKISREKNRALIG
                     HTLDVLVEKPAASGEAAVGRSHREAPEIDGVIRITGQAGQPGNFSRVRITGANAFDLT
                     GVAVPDRTRHPREGAPIAVVR"
     gene            1342..2247
                     /locus_tag="VF720_12905"
     CDS             1342..2247
                     /locus_tag="VF720_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880306.1"
                     /translation="MLPRLAMAQPDTSRVVLADRALVFDTNLGLIQRATEEAIDRALT
                     GLKLPDNSEIRLFSVTARDGDWFVEDRIAHYLSSRGYKVYLVEKPKPGSLGGATTQPG
                     GAALSMGAALNATAGSGAPNTSTSNDSTGSTPDSTATDPDQEPPADEPPPHTSGPPNR
                     PTGPRNAPGVQAADQPIETMNEFPEDIEGLVLSVRVLEFAVTYHDQWRQGFMGQRVVE
                     RLAAVDLNCRLVQGSQKSILWVGNGRSEKLDIVPKSKLDLLEGRSYPFGKPSLPPQSL
                     SRIVEPALVLGIVAGLVFLFYSNQN"
     gene            2260..3150
                     /gene="bamD"
                     /locus_tag="VF720_12910"
     CDS             2260..3150
                     /gene="bamD"
                     /locus_tag="VF720_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEX7880307.1"
                     /translation="MRNHPAAAIPISMAAWRRPVRAGVLLALWLLASGLGACATSKPD
                     FAIPPSMTDDPLAPAKADLAGGNSLKATERLTKFLQENPGSIHVDEANYLLGLAYLDQ
                     KDRVLAADYFQKVTRDFPTSPLASESSYYLALSYDHLSRPSSLDQDWTERAIGSYRLF
                     LAKYPQHARVPEVEARIHALEDRLAQKLYDNGALYLRMGRPTSAEVYFQRVIEQYSQS
                     SFACKAATGLGQTYMKRRRWQEAVTQFEQVVDSCPDTDAGKKARDLLEESRTEAGRLA
                     AVPAAAPVDTAGPPDSASAP"
     gene            3173..3760
                     /gene="nadD"
                     /locus_tag="VF720_12915"
     CDS             3173..3760
                     /gene="nadD"
                     /locus_tag="VF720_12915"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX7880308.1"
                     /translation="MTIGVFGGTFDPIHWGHLVLAQEAMNAAGLERILFVPSAVPPHK
                     QGRELTSWKHRMAMVRLAVDGIPGFEVSDVESDPGRPHYSLDTLERLAEHHPGDRLSF
                     IIGSDSLLEMPGWRSPSAIVERWPLVVLARPGFDAAPATPAYTTNMTLVDGVQVTVSS
                     TLIRRRLAGGQKVNFLLPDTVIEYARRNGLYGMKS"
     gene            3757..6627
                     /gene="polA"
                     /locus_tag="VF720_12920"
     CDS             3757..6627
                     /gene="polA"
                     /locus_tag="VF720_12920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX7880309.1"
                     /translation="MTDAPRRPRLFLVDGTALAYRAFFAMGRSGLSTSSGEPTGAVFG
                     VANTLWSLLAEFRPEYMAVVFDTAAPTFRHEEYAEYKANREEMPSDLASQLPRLSEMV
                     KAWPLVHLAVDGVEADDVMGSYAVQLAGPDLEVVLVTADKDFCQLVNDNVFILNPGRG
                     GPAKVEAHRLDAKGVEAKFGVPPAQVIDVLALMGDTSDNVPGVPGIGEKSAAQLIREF
                     GSLEALLGSIDKVSRKSWAEKLTTWAEQARLSRRLVTIKTDCPLPASLAEMAVGKPAP
                     SLAEFFQRMEFGRLTRQATEAAGLTTAGLVESLAPAAVRESGPEPAAPTAMGATMDLF
                     APPGAEGTMVAAPAGPWLTEPPPTDAEYRLADDERALVRLAAELRAAPGYVSIDTETT
                     GLDPMRAALVGLSFSTVGKTGWYVPVGHVEGPNVSLEQVRTHLGPVLADPSVKKVGQN
                     IKYDLVVLDRHQLPVAGVAFDTMVASYLIDAERSHKLDNLAHDELGYRMVPITDLIGK
                     GQKQRTFADVPVPLAVRYAAEDADITLRLSDLFLPRLAERELESLFRDIEMPLLLVLK
                     EMEETGIALDVPFLNAMSERLGREIAKLESFAHETAGETFNLNSPIQLGKLLFEKLKL
                     PAGRKTKTGWSTDSETLEDLVPQHALPKLILDYRQLAKLKSTYVDALPRLVHPDTGRV
                     HSCFNQTVAATGRLSSSDPNLQNIPIRTELGREVRRAFVAGGPGMSLISADYSQIELR
                     IMAHLSGDANLNDYFRRGVDVHRETAASIFGVPAAEVTGEQRAQAKTVNFGIMYGQGP
                     VGLSRQIGITREAAKQFIAAYKAQFPGVTTFLDRTVREATSSGYVTTLMKRRRYLPNL
                     LSGEGRFRAEAERMAINTPIQGTAADLIKLAMLSVDRELKDRYPRARLLLQVHDELVL
                     EAPDAEAEAVAATVRSAMENAVTLSVPVVVDVGVGRDWAGIH"
     gene            6679..7335
                     /gene="coaE"
                     /locus_tag="VF720_12925"
     CDS             6679..7335
                     /gene="coaE"
                     /locus_tag="VF720_12925"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX7880310.1"
                     /translation="MLSVGLTGNIASGKSMVAAQLESLGAVVIDADRIGHVLIGSGGA
                     AVDEVRAAFGPGVVGQEGGIDRALLGPVVFADPAARERLNAIVHPRLVAEIRRRMEGM
                     ASAESREDWEGGPPVPLILVVDAALIFELDMAGDFDVVVVVSAPEAVREARLVAKGLT
                     AEEARRRIASQWPEVEKVRRADHVIVNDGAPERLAADVEQLWKILTRAAASPSHRKDR
                     "
BASE COUNT         1200 a         2550 c         2471 g         1158 t
ORIGIN      
        1 gcgctttcct gcaggcggcg gtggaggagg ggatcgacac catcgtcgat gtggcccgac
       61 tcaaggccgc aaatctaaag gggctggtcg tgacaggctg catgtcccag cgctattcgg
      121 ggcagctcct tacggagatc ccggagatcg acgcgatcgt gggtaccggc gcggtcgacg
      181 agatcgtcga ggtctgccga aacgtgctcg acgagaaggg tgtgcgcacc gagctggtcg
      241 gcgaccccaa ccgctcgtat ctcgacccgg cggtgcgcgt tctcagcacg ccgcgctgga
      301 gcccgtatct gcaagtgagc caggggtgct cgaacacctg cacgttctgc atcctcccca
      361 agctgcgcgg cgacggccgg agccgctcac tggaggatgt ggtgggggag gcccgccggc
      421 tggttcaatc cggcgcccgc gagatcgtcc tcatcgggca ggacacgacg gcctggggtc
      481 tcgacaccga gggtcgcgcc ttcctggccg actgctaccg ccggctcgac gagctggaag
      541 gggatggcct caagtgggtg cgggtgatgt acacgaatcc gctcttctgg gacgaggcgc
      601 tggtggagag cttcggcgcc tcccgcaccg cctgctcgta catcgacatg ccgatccagc
      661 actccgaaac ccgcgtcctc gaacggatgg ggcgcggcta cacgcgtgaa cactgcgcat
      721 ggctggttcg gcgacttcgg gaagtgcgcc cggacgtggc gctccggacc tccatcatcg
      781 tggggttccc gggcgagacc gaggcggaat tcgaagccac gctggctttc ctcgaggaag
      841 cccgcttcga ccggctggtg gcctttccct acttcccgga agagggtacg gccgcgttca
      901 agctggacgg ccgggtgccc gacgacgtcc agcagcgccg ggtggccacg ctccttgacc
      961 tgcagggaaa gatttctcgg gagaaaaacc gcgccctgat cggtcacacg ctcgacgtcc
     1021 tggtcgagaa gcccgctgca tccggcgagg ctgccgtcgg ccgcagccac cgcgaggcgc
     1081 ccgagatcga cggcgtcatc cgcatcacag gtcaagccgg ccagcccggc aatttctccc
     1141 gcgtccggat cacgggcgcc aatgccttcg acctgaccgg cgttgccgtg ccggacagga
     1201 cccgccaccc gcgcgaagga gccccgattg ccgttgtccg ctgaccgtcc ccgcgacgtg
     1261 ggtggcctcg tgcccccggc gccaggcagg ttccggccac tcatcgcccc gttcatcgct
     1321 cttttgtttc tcgccggcct catgcttccg cgactcgcca tggcgcagcc cgacaccagt
     1381 cgggtcgtgc tggcggatcg cgcgctcgtg ttcgacacca acctcgggct catccagcgg
     1441 gccacggagg aggccatcga ccgggcgctc accggcctca agctccccga caattcggag
     1501 atccggctct tctcggtgac ggcgcgcgac ggcgactggt tcgtggagga ccggatcgcc
     1561 cattacctgt cgtcgcgcgg ctacaaggtg tatctggtgg agaaaccgaa gccggggagc
     1621 ctgggtggcg cgaccacaca accgggaggc gccgcgctct ccatgggggc ggcgctcaat
     1681 gccaccgccg gttcgggcgc gccgaacact tcgacctcga acgactccac cggaagcacg
     1741 ccggactcca ctgcgacgga cccggaccag gagccgccgg ccgatgagcc accgccgcac
     1801 acttcggggc cacccaaccg tccgaccggt ccgcgaaacg cgccgggagt gcaggccgcc
     1861 gaccagccga tcgaaacgat gaacgagttt ccggaggaca tcgaaggact ggtcctgagt
     1921 gtccgcgtgc tcgaattcgc cgtcacctac catgaccagt ggcgccaggg attcatgggg
     1981 cagcgcgtcg tggaacgatt ggccgccgtc gacctcaact gccggctcgt ccagggaagc
     2041 cagaagagca tcctctgggt cgggaacggc cgatcggaaa aattggacat cgtgccgaaa
     2101 tccaagctcg acctgctcga ggggcgaagc tacccgttcg gcaagcccag cctgccgccc
     2161 cagtcgctgt cgcggatcgt cgagccggcg ctcgtgctcg ggatcgtggc cggcctggtc
     2221 ttcctcttct actccaacca gaactaggat caggagatca tgaggaacca ccccgcggcc
     2281 gcaatcccca tttccatggc cgcatggcgc cggccggttc gagcgggtgt gctcctggcc
     2341 ctctggctcc tggcctccgg cctcggggcc tgcgccacct ccaagccgga cttcgcgatt
     2401 cctccctcga tgacggacga tccgctggcc ccggccaagg ccgatctggc cggcggcaac
     2461 tcgctcaagg ccaccgagcg actcaccaag ttcctgcagg agaatccggg gagcatccat
     2521 gtggacgagg ccaactacct cctcggcctc gcatacctgg accagaagga ccgcgtgctc
     2581 gccgccgact atttccagaa ggtcacgcgc gactttccca cgagcccact ggcctcggag
     2641 tcgagctatt acctggcgct ctcgtatgac catctctccc gcccgtcgtc gctcgaccag
     2701 gactggaccg agcgggccat cggctcgtat cgcctcttcc tggccaagta tccgcagcat
     2761 gcacgggtgc cggaggtcga ggcccggatc cacgcgctgg aagaccgatt ggcgcagaag
     2821 ctgtacgaca acggcgccct ctacctgcgc atggggcgcc cgacctcggc ggaggtgtac
     2881 ttccagcgcg tcatcgagca gtactcgcag tcgagctttg cctgcaaggc ggcaacggga
     2941 ctcgggcaga cctacatgaa gcggcggcgc tggcaggaag ccgtcactca gttcgagcag
     3001 gtcgtcgact cctgtcccga caccgacgcg gggaagaagg cgcgcgacct cctcgaggag
     3061 tcgcgcaccg aggccggccg cctcgccgcc gtcccggcag cggctccggt cgatacggcc
     3121 gggccgccgg actccgcttc cgcgccctag gcgccagggg acgggctcgg ccatgacgat
     3181 cggcgtcttc ggcggcacct tcgatcccat ccactggggt catctcgtgc tggcgcagga
     3241 ggccatgaat gccgccggcc ttgaaaggat cctcttcgtg ccgtccgccg ttccacccca
     3301 caagcagggt cgcgagctca cctcctggaa gcatcgcatg gcgatggttc gactggccgt
     3361 cgacgggatc ccggggttcg aggtttccga cgtcgaatcc gatcccggcc ggccacacta
     3421 cagcctcgat acgctcgagc ggctcgcgga gcatcacccg ggtgatcggc tttccttcat
     3481 catcgggtcg gacagcctcc tcgagatgcc ggggtggcgt tccccgtccg cgatcgtgga
     3541 acggtggccc ctggtggtgc tggcccggcc cggcttcgac gcggcgccgg caacgcccgc
     3601 gtacaccacc aacatgaccc tggtggacgg cgtccaggtg accgtgtcat ccaccctgat
     3661 ccggcgccga ctggccggcg gacagaaggt gaacttcctc ctgccggaca ccgtgatcga
     3721 atacgcgcgc cggaacgggc tctacgggat gaaatcgtga ccgacgcacc ccgccgcccc
     3781 cggcttttcc tcgtggatgg caccgcgctg gcctaccggg ccttctttgc catgggacgc
     3841 tccggcctct cgacgtcctc gggtgaaccc accggcgccg tgttcggcgt ggccaacacg
     3901 ctctggagcc tgctcgccga gtttcggccc gagtacatgg cggtggtctt cgacaccgcc
     3961 gcgcccacct tccgccacga ggagtacgcc gagtacaagg ccaaccgtga ggagatgccg
     4021 tccgacctgg ccagccagct gccgcgactc tcggagatgg tgaaagcctg gccgctcgtg
     4081 cacctggccg tggatggtgt cgaggctgac gacgtgatgg gttcgtacgc cgtgcagctg
     4141 gccgggccgg acctggaggt cgtcctcgtc accgccgaca aggatttctg ccagctcgtc
     4201 aacgacaacg tcttcatcct gaatcccggc cggggggggc cggcgaaggt ggaggcgcac
     4261 cggctcgacg cgaagggggt ggaggccaag ttcggggtgc cgccggcgca ggtcatcgac
     4321 gtgctggccc tgatgggcga tacctccgac aacgtgcccg gcgttcccgg gatcggcgag
     4381 aagtcggcgg ctcagttgat ccgcgagttc ggctcactcg aagcgttgct gggctcgatc
     4441 gacaaggtct cccggaaatc gtgggccgag aagctcacca cctgggccga gcaggcccgg
     4501 ctctcgcggc ggctggtcac gatcaagacg gactgcccgc tgccggcctc gctggccgag
     4561 atggccgtgg ggaagccggc gccctcgctg gccgaattct tccagcggat ggagttcggg
     4621 cgactcaccc gccaggcgac cgaggcggcg ggactcacca ccgcgggcct cgtggagtct
     4681 ctggcgccgg cggccgtgcg ggagagcggc cccgagccgg cagccccgac agccatgggc
     4741 gccacgatgg atctcttcgc gcccccgggg gccgagggca ccatggtggc ggcgcccgcc
     4801 gggccgtggc tcaccgagcc gcctcccacc gatgccgagt accgactcgc ggacgatgaa
     4861 agggcgctcg tccggctggc cgccgaactc cgggcggcgc ccggatacgt gtcgatcgac
     4921 accgaaacaa ccgggctcga cccgatgcgc gcggcgctgg tgggcctgtc tttttccacc
     4981 gtcgggaaga ccgggtggta cgtccccgtc ggccacgtcg aggggccgaa cgtgtcgctc
     5041 gagcaggtgc gcacgcacct ggggcctgtc ctggccgacc cgtcggtgaa gaaggtcggg
     5101 cagaacatca agtatgacct cgtcgtcctg gatcgccacc agctcccggt agccggcgtg
     5161 gcgttcgaca ccatggtggc gtcgtacctg atcgacgcgg agcgctcgca caagctcgac
     5221 aacctcgcgc acgacgagct cggctaccgg atggttccca tcaccgacct gatcggcaag
     5281 gggcagaagc agcggacgtt cgccgacgtg ccggtgccgc tggcggtgcg ctatgccgcg
     5341 gaggacgccg acatcacctt gcggctctcc gacctgttcc tgccgaggct ggccgagcgg
     5401 gaactcgagt ccctctttcg cgacatcgag atgccgctgc tcctcgtgct caaggagatg
     5461 gaggagaccg gcatcgccct ggacgtcccc ttcctcaatg ccatgtcgga acggctcggc
     5521 cgcgaaatcg cgaagctcga gagcttcgcc catgagaccg ccggcgagac attcaacctg
     5581 aattcgccga tccagctcgg gaagctcctc ttcgagaagc tgaagttgcc ggccggccgc
     5641 aagaccaaga ccggctggtc gaccgactcg gaaacgctgg aagacctggt gccacagcac
     5701 gcgctgccga agttgatcct cgactaccgg cagctggcca agctcaaatc gacttacgtg
     5761 gatgcacttc cgcgcctcgt tcaccccgac accgggcggg tgcacagctg cttcaaccag
     5821 acggtggccg ccaccgggcg tctctcgtcc agcgatccca acctccagaa catcccgatc
     5881 cggaccgaac tcgggcgcga ggtgcggcgg gcgttcgtgg ccggtggccc gggcatgtct
     5941 ctcatctcgg ccgactattc gcagatcgaa ctccggatca tggcgcacct gtcgggcgac
     6001 gcgaatctca acgactattt ccggcgcggc gtggatgtgc accgcgagac ggcggccagc
     6061 atcttcggcg tgccggccgc cgaggtgacc ggggagcagc gcgcgcaggc caagacggtc
     6121 aacttcggca tcatgtacgg acaggggccg gtgggccttt cgcggcagat cggcatcacc
     6181 cgcgaggcgg ccaagcagtt catcgcggcc tacaaggccc agtttcccgg tgtcaccacc
     6241 ttcctagacc ggaccgtccg ggaggccacg agctcgggct acgtcaccac gttgatgaaa
     6301 cgacgacgct acctgccgaa cctcctgagc ggggaaggac gcttccgggc cgaggccgag
     6361 cgcatggcca tcaatacgcc gatccaggga acggccgccg acctgatcaa gttggccatg
     6421 ctctcggttg accgcgagct caaggaccgg tacccgcgcg cccggctcct cctgcaggtg
     6481 cacgacgaac tggtgctcga agcgccggac gccgaggccg aggcggttgc ggccaccgtt
     6541 cgctcggcca tggagaacgc cgtcacgctc tcggtgccgg tggtcgtgga tgtgggggtc
     6601 gggcgcgact gggccggcat ccattgacac gcgccggggg tgacgccccc cggcagcatc
     6661 cactatgatg ggccgatcat gctcagtgtc ggcctgacgg gcaatatcgc gtccggcaaa
     6721 tccatggtgg ccgcgcaatt ggagagcctc ggcgccgtgg tcatcgacgc ggaccggatc
     6781 ggccacgtgc tgatcggttc gggtggcgcc gctgtcgacg aggtccgggc cgcattcggt
     6841 cccggcgtgg tcggacagga aggcggtatc gaccgcgccc ttctcggccc ggttgttttc
     6901 gccgacccgg ccgcgcgcga acgactcaac gccatcgtcc acccccggct cgtcgcggaa
     6961 atccggcggc gcatggaagg gatggcgtcg gccgagtcgc gcgaggactg ggaagggggg
     7021 ccgcccgtcc cgctcatcct ggtggtcgat gcggcgctga tcttcgagct ggacatggcc
     7081 ggcgatttcg acgtggtggt cgttgtctcg gcgcccgagg ccgttcgcga ggcccgcctc
     7141 gtggccaagg ggctcacggc cgaggaagcc cgccggcgca ttgcgagcca gtggcccgag
     7201 gtggaaaagg tccggcgggc cgaccatgtg atcgtgaacg acggtgcgcc ggaacgcctg
     7261 gccgccgacg tcgaacaact ctggaagata ctgacgaggg cggccgcatc gccctcccat
     7321 cgaaaggatc ggtagcgatg cccgtcgacg tgcaggactt gaagaagacg atggccacc
//
LOCUS       DASVOD010000586         2863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_715076, whole genome shotgun sequence.
ACCESSION   DASVOD010000586 DASVOD010000000
VERSION     DASVOD010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715076"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="VF720_12930"
     CDS             complement(<1..819)
                     /locus_tag="VF720_12930"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEX7880311.1"
                     /translation="MPFSFLNMAFLAGLGAAVIPIVIHLFSRKRQKRVEFSSLQFIEE
                     IAKRRVRRVTITQWLILALRVLVLSLVALALMRPALKGDFALGKSKGESSVALILDRS
                     FSLRAATDTGLLFDEAKRRAVEVAEAIDDGDEIHFLGATPGLETPAPPFHDRLRLKEE
                     IQALEPGWGSTDLVEAIREAGTRLRKSEALNHELYIVSDFQKSGFGDLAGNVGTQIAD
                     LVAKDVRVYLLPLSEETIDNRAIESISFEGIPPNRNARALVANHGGKSDPDVVIT"
     gene            1146..1844
                     /locus_tag="VF720_12935"
     CDS             1146..1844
                     /locus_tag="VF720_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013088788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX7880312.1"
                     /translation="MITVRPSAERGHVNFGWLDTFHTFSFGEYHDPSHMGFRSLRVLN
                     DDRVDGGAGFPAHGHRDMEILTWVLDGALEHKDSMGNGSVIRPGQLQFMRAGTGVRHS
                     EFNHSKSEPVHLLQIWIIPEKPGLEPAYGELTPDKDALARDFVTVASRDGRDGSVRIA
                     QDAALAITRLAPNDRRTRTVPAGRHAWLHVATGAITLNGTALTAGDGAAISGAETLDI
                     VAGTDAEVLLFDLG"
     gene            complement(1866..2420)
                     /locus_tag="VF720_12940"
     CDS             complement(1866..2420)
                     /locus_tag="VF720_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014083408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HEX7880313.1"
                     /translation="MNAPDPRADFLADSIDEFRRMKKLAEKSLARMTDEQFFQPLDAE
                     SNPAALVVKHMAGNMRSRWRDFLTTDGEKDDRNRDTEFELDAADTRTALMERWEAGWA
                     LCFGALEPLGPDDLSRHVLIRSEPHTVTRAVIRQLTHYSYHVGQIVTLAKHAASSDWQ
                     SLSVPRGQSDEFNRKMLEKFPAKG"
     gene            complement(2442..>2863)
                     /locus_tag="VF720_12945"
     CDS             complement(2442..>2863)
                     /locus_tag="VF720_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880314.1"
                     /translation="TDDYYPDYYYPYEPVEKHGGFVGRVGLFSHFEPWLAVGAETGFM
                     TTEPHAWRLAPAVRIDPFPPTLAARPYLAGSIGGSFWENGESLFSSDVGIGVEHPLQQ
                     GRSALGVESRYYFNLQNAVESGTYTHVSVALTWRHDW"
BASE COUNT          489 a          937 c          938 g          499 t
ORIGIN      
        1 cgtgatcacg acatcgggat cgctcttgcc gccgtgattg gccaccagcg cgcgggcatt
       61 gcgattcggc gggattccct cgaacgagat cgactcgatg gcccggttgt cgatcgtttc
      121 ctcggaaagc ggcagcaggt agacccgcac gtccttggcc acgaggtcgg cgatctgggt
      181 gccgacgttg ccggccaagt cgccgaagcc cgacttctgg aagtcgctga cgatgtagag
      241 ctcgtggttc agcgcctcgc tcttccggag gcgcgtgccc gcctcgcgga tggcctcgac
      301 caggtcggtg ctcccccagc cgggttcgag cgcctggatc tcttccttca accgcagccg
      361 atcgtgaaac ggcggggccg gcgtttcgag gccgggagtg gcgccgagaa agtggatctc
      421 gtcgccgtca tcgatggctt cggccacctc caccgcgcga cgcttggctt catcgaacag
      481 gaggcccgta tcggtggccg cgcgcaggct gaacgaccgg tcgaggatca gggcgacgga
      541 gctctctccc ttcgacttgc cgagggcaaa gtcacccttc aaagccggtc gcatcaacgc
      601 gagcgccacc agcgaaagca ccagcacccg cagcgccagg atgagccact gcgtgatggt
      661 gacgcgccga acacgccttt tcgcgatctc ctcgatgaac tgcagcgagg aaaactccac
      721 ccgcttctga cgcttgcggc tgaagaggtg gatgacaatc gggatgaccg cggcgcccag
      781 cccggccagg aacgccatat taaggaagga aaacggcatc gttccttctg gttaagtaat
      841 tgtcatttag cggtttatgc tccgcgctgg cggggtttgc ggagccgagc gggaatgcta
      901 gcaggtgccc caccctcaag gaaggggcgg ggttccgggg tcgaacgggc gcggagccgg
      961 ctaactcgct tcaaagtcag caattccgat atgtcgcaaa cattctgtca tgctgggatg
     1021 cgggtcccct cttctatagt ggcgatcccc ctggaatccg cgtccgtcca tccccactcc
     1081 aattcgtgtt gaaacacaca ggccgggtga cgtaccgggc ctgatcacca gcaacgggag
     1141 tgtccatgat caccgtccgt ccatccgccg agcgcggcca cgtcaacttc ggttggctcg
     1201 atacatttca caccttctcg ttcggtgagt atcacgaccc gtcgcacatg ggattccgtt
     1261 cgctgcgcgt cctcaacgac gaccgtgtcg acggcggcgc cggcttcccc gcccacggcc
     1321 accgcgacat ggagatcctc acctgggtgc tcgacggcgc gctcgagcac aaggacagca
     1381 tggggaacgg ctcggtcatc cggccggggc agctccagtt catgcgggcc ggcacgggcg
     1441 tgcgccacag cgagttcaac cactcgaagt ccgagccggt gcacctgctc cagatctgga
     1501 tcatcccgga gaagcccggc ctcgaacccg cctacggcga actgacaccg gacaaggacg
     1561 cgctggcgcg cgacttcgtg acggtggcct ctcgcgatgg acgggacggc tcggtccgga
     1621 tcgcgcagga cgcggcgctg gccatcaccc ggctcgcacc gaacgaccgg cgcacccgca
     1681 ccgtgccggc cgggcggcat gcctggttgc acgtggccac gggggcgatc acgctgaacg
     1741 ggacggcact gaccgccggt gacggcgcgg cgatctcggg cgcggaaacg ctcgacatcg
     1801 tggccggcac cgacgcggaa gtgctgctct tcgacctggg atgaggatca cttgtttcga
     1861 gcgccctacc ccttcgccgg aaacttctcc agcatcttcc gattgaactc gtccgactgg
     1921 ccacggggca ccgagaggct ctgccagtcg gacgaggccg cgtgtttggc cagcgtcacg
     1981 atctgcccca cgtggtagct gtagtgggtg agctgccgga tgacggcgcg cgtcaccgtg
     2041 tgaggctcgg accggatcag cacatgacgt gacaggtcgt ccggccccag cggctccagc
     2101 gctccgaaac agagggccca gcctgcctcc catcgctcca tgagggccgt gcgcgtgtcg
     2161 gcggcgtcga gctcgaactc ggtgtcgcgg ttgcggtcat ccttctcgcc gtcggtcgtc
     2221 aggaagtcgc gccagcggga acgcatgttg ccggccatgt gcttgaccac gagggcggcg
     2281 ggattcgatt cggcgtcgag cggctggaag aactgctcat cggtcatgcg ggccagcgac
     2341 ttctcggcca gcttcttcat cctgcggaat tcatcgatcg agtcggccag gaaatcggcg
     2401 cggggatcgg gtgcgttcac gcgtgactct ccttctcggg cctaccagtc gtgacgccac
     2461 gtcagggcca cgctgacgtg cgtgtaggtt ccggattcga cggcattctg gagattgaag
     2521 tagtaacggc tctccactcc cagggccgac cgcccctgct ggagcggatg ttccacgccg
     2581 atccccacgt cgctcgagaa gagcgattcg ccgttctccc agaaactgcc gccgatgctc
     2641 ccggccaggt aagggcgtgc cgccagcgtc ggcgggaagg ggtcgatccg cacggccggc
     2701 gccaggcgcc acgcgtgagg ttccgtggtc atgaaaccgg tctcggcgcc gaccgcgagc
     2761 cagggctcga agtgggagaa gagtccgacg cggccgacga atccgccgtg cttctccacg
     2821 ggttcgtacg ggtagtagta atccgggtaa tagtcgtcgg tga
//
LOCUS       DASVOD010000587         5285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_247437, whole genome shotgun sequence.
ACCESSION   DASVOD010000587 DASVOD010000000
VERSION     DASVOD010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247437"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VF720_12950"
     CDS             complement(<1..358)
                     /locus_tag="VF720_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880315.1"
                     /translation="MCFGAALAAIAPVVIVVAALAAYDPIGWSSIDGGGTMGATAGGW
                     TLSGTIGQYDAGSHVGGANTLGGGFWQGGLAAVVGVPEGPVVPVRTFRFWPAAPNPVR
                     GACRLSFDLAAPAPARL"
     gene            complement(367..1455)
                     /locus_tag="VF720_12955"
     CDS             complement(367..1455)
                     /locus_tag="VF720_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880316.1"
                     /translation="MRIPRTNLDFVRPAILLALLGMATLVNAAPLGRSFTYQGKLSQA
                     GTPVTGAVTLRFSLWDAAGTGDPPTGGVIVGAFQILPDVAVTEGLFTVVLNSGGEFGA
                     TAFNGQERWLQAEVCEDGTCAETTVLGPRQSMTGAPYALGPWQLSGSHISYTNGNVGI
                     GTSTPAVPLHIMTPDEGLRIQGTSGTAWASFYNAAGTALGYVGDGSGSDANIYLASYG
                     NDVILYTSTAALTAKANGNIGIGTSAPAEKLEVRGNIKLGTSGTLFAPSATENLRMLR
                     GRVSSAGAVLTGTGFTSSRTNVGIYNIAFSSTFPVAPVLVAAAEAVGTPYYATVSFTT
                     TTQAAVRTFNASGAPADIGFSFMIAGTR"
     gene            complement(1525..3330)
                     /locus_tag="VF720_12960"
     CDS             complement(1525..3330)
                     /locus_tag="VF720_12960"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX7880317.1"
                     /translation="MRPLFCFADTARVIAALALTLVVPSSVYAQCGDRQITCVDDIVV
                     TECGTHGAVINYTAPTASDSCGPVATYLVEGYAPGSVFPFGTTTVEWRTGYDDNDHFE
                     RCIFTVTVLVSPRTLVCPGDTVIVACGNFGPVVNYPQPVAYDSCGPVPTYLYEGLPPG
                     SNFPFFTTTVEWQTDYDGNENIESCTFHVTVQASRRTIECPDDIVVTGCSSEGAVVNY
                     LQPTAYDMCGPVPTYLYQGLPSGSVFPYGTTIVEWQTDYDGQERIENCDFTVTVLEDT
                     EPPAITCLTGVTAVASDGGTSAVVNYDVEVTDGCPDGLVVSYQPPSGTTLPCGITPVT
                     VTARDAGGRESTCRFNATVSLPVPVDILPAACPNPLNVATKGVVPVAIMGSSLFDVAR
                     IDPASVRLEGVAPAGWSRTDVGAPFLPMLGKGDCRDCSASRKDKRGDVLFRFDQTAVV
                     AALGPVADGACRTLRLTGATRDGCPIFGEDVVRLQVGGTIPAGDGASVTLVPVTAPRA
                     PVLHAARPNPLAQATAIEFELPTAATVRLEVFDVVGRPVRLLLNEPRTAGRQAAAWDG
                     ADDAGRPVADGVYFYQLTVVEGTPFREVRKLVVSR"
     gene            complement(3349..>5285)
                     /locus_tag="VF720_12965"
     CDS             complement(3349..>5285)
                     /locus_tag="VF720_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7880318.1"
                     /translation="PMALATGRRTVPALGNAMQNLTWTIELPFPGPVYWSVQAIDGAF
                     IGSPFATEQSLCVAGFADVASGVTGTAGGASAWGDYDNDGDLDLVVSGTPDGSTYFTE
                     VYRYDGVFGQPLYNTVNAVLPGTVYGAAAWGDMDNDGDLDLALSGNTGSGPITRVYRQ
                     SFGLFVDIGAGLPGTFFGSLAWGDIDNDGDLDLVVTGNDGTIGISRIFRSSGGVDPVF
                     TDAAAGLIGLTYSAVSLCDFDVDGDLDILMTGRTAGGGGSSTTAMLYRSSGGANPTFA
                     GVPTGITGVINGSHAWGDYDADGDPDLVVCGEATGPVKVARLWRNPGGPTPTFGTPIA
                     SFTGVVSGSAAFGDHDNDGDLDILIAGNDGSGSSARLYDNGGGANPVFTVSADPLPGV
                     YYGSAAFGDMDKDHDLDIVLSGLPGAPYATKVMRNTCRPLNTPPQPPSNLSVSMVGNR
                     ATFSWNAAVDNNTPAAAFTYNIRVGSTSGGSEIVSPMAMPTSGLRRIAARGNTGQRTN
                     CTIDLPGGGPWYWSVQAIDAAYEGSPFATEQAIAQSGVSAEAAPTVTQLWSAGPNPFS
                     GVASIGFSVAAKGPVSVTIIDPAGRLVRRLIDGELEPGRFDRSWDGRDDAGTELPSGL
                     YFARLRAGNDSRVLRLLLIK"
BASE COUNT          839 a         1780 c         1853 g          813 t
ORIGIN      
        1 cgaggcgggc cggcgccggg gccgcaaggt cgaacgacaa ccggcaggct ccgcgcacgg
       61 ggttgggcgc cgcgggccag aagcggaagg tgcgcaccgg cacgaccggc ccctcgggta
      121 cgcccaccac cgcggcgagc cccccctgcc agaagccgcc gcccagagta ttcgctccgc
      181 cgacgtgcga tcccgcgtcg tactgaccga tggtgccgga gagcgtccac cctccggcgg
      241 tggcgcccat ggtgccgccg ccgtcgatgg agctccagcc gatggggtca tatgcagcca
      301 gcgcggcaac gacgatgacg acgggcgcga tggccgcgag cgccgctccg aaacacatcg
      361 cccgtctcat cgcgtgccgg caatcatgaa ggagaatccg atgtccgccg gggcgcccga
      421 ggcgttgaac gtccgcacgg cggcctgggt ggtggtggtg aagctgaccg tggcgtagta
      481 cggcgttccc acagcctcgg ccgcggccac gagcaccggc gccacgggaa acgtgctgct
      541 gaacgcgatg ttgtagatcc ccacgttcgt ccgggaagac gtgaagccgg ttcccgtcag
      601 caccgcaccc gccgaggaga cgcgccctcg aagcatcctc aggttttccg tggcgctcgg
      661 ggcgaagagt gtgccgctcg tcccaagctt gatgttcccg cggacctcga gcttttccgc
      721 gggagcggag gtaccgatgc cgatgttgcc gttggctttc gcggtcaggg cggcggtgga
      781 ggtgtagagg atcacgtcgt tcccgtagga ggccaggtag atgttggcgt cggaaccgct
      841 gccgtcgccc acgtagccca gggccgtgcc ggcggcgttg tagaagctgg cccacgctgt
      901 tccggacgtc ccctggatgc gcagcccctc gtccggtgtc atgatgtgga gcggcacggc
      961 gggcgtgctc gttccgatgc cgacgttccc gttcgtgtag ctgatgtggc ttcccgagag
     1021 ctgccacggc cccagtgcgt agggcgcccc cgtcatcgac tgccgcgggc cgagcacggt
     1081 ggtttccgcg caggtgccgt cctcgcacac ctccgcctgc agccaccgct cctggccgtt
     1141 gaaagcggtg gcgccgaact cgcctcccga gttcagcacg accgtgaaga gtccctcggt
     1201 caccgccacg tcgggaagaa tctggaatgc gccgacgatc acgccgcccg tcggcggatc
     1261 gcccgtgccg gccgcgtccc acaggctgaa acgaagtgtc accgcgcccg tcaccggcgt
     1321 gccggcctgg ctcaactttc cctggtaggt aaaggagcgg ccaagcggcg cggcgttgac
     1381 gagcgtggcc atcccgagaa gggctagcag gatggccggt cgcacgaaat cgaggttggt
     1441 tcgagggatt cgcatggctg ttcctcttgg gttatgtcgg cgcagccgac gacctcaacg
     1501 gctgacgatg attcgttccc ggtgtcaccg gctgaccacc aacttccgca cctcccggaa
     1561 cggtgttccc tcgaccacgg tcagttggta gaagtagacg ccatccgcca ccggacgccc
     1621 ggcgtcgtcg gcgccatccc acgcggcggc ctgccgtccg gcggtacgag gttcattcag
     1681 cagcaggcgg accggccgac cgaccacgtc gaagacctcg agacggacgg tcgcggcggt
     1741 cggcagctcg aattcgatgg ccgtggcctg cgcgagcggg ttgggacggg cggcgtggag
     1801 cactggcgcc cgcggcgccg tcaccggcac cagcgtcacc gaagcaccgt ccccggccgg
     1861 gatcgtgccg cccacctgga gccgcaccac atcctcgccg aagatcgggc aaccgtcgcg
     1921 ggtggctccg gtgagtcgca gggtgcggca cgcgccgtcg gccacggggc cgagggcggc
     1981 caccacggcc gtctggtcga accggaaaag cacgtcgccc cgcttgtcct tgcgcgaggc
     2041 gctgcaatcg cggcaatccc ccttgccgag catgggcagg aacggcgcac cgacatctgt
     2101 gcgggaccat cccgcgggcg ccactccttc caggcgcacg ctggccgggt cgatgcgggc
     2161 cacgtcgaac aaggacgatc ccatgatcgc caccggcacg acgcccttgg tggccacgtt
     2221 cagcgggttg gggcaggcag ccggcaggat gtcgaccggc accggcaggc tcaccgtcgc
     2281 gttgaagcgg cacgtgctct cgcggccccc cgcgtcgcgg gccgtcacgg tgaccggcgt
     2341 gatgccacag gggagcgtcg tgccgctcgg cggctggtac gagacgacca ggccgtcggg
     2401 gcacccgtcg gtgacctcga cgtcgtagtt gacgacggcc gaagtcccgc catccgaggc
     2461 caccgccgtc acgcccgtca ggcaggtgat cgccggcggc tccgtgtcct cgagcaccgt
     2521 taccgtgaag tcgcagttct cgatgcgctc ctggccgtcg tagtcggtct gccactcgac
     2581 gatcgtcgtg ccgtacggaa agaccgaccc ggatggcagc ccctggtaga gatacgtcgg
     2641 caccggtccg cacatgtcgt aggcggtggg ctgcaggtag ttcacgaccg cgccttccga
     2701 cgaacagccg gtcaccacga tgtcgtccgg gcattcgatg gtccggcgcg aggcctggac
     2761 ggtcacgtga aacgtgcaac tctcgatgtt ctcgttgcca tcgtagtcgg tctgccactc
     2821 gactgtggtg gtgaagaacg gaaagttcga cccgggggga agtccctcgt acaggtacgt
     2881 cggaaccgga ccgcaggagt cgtaggccac cggttgcggg tagttcacga ccggaccgaa
     2941 gttgccgcac gccacgatga cggtatcccc cgggcagacc agggtgcgcg gcgataccag
     3001 cacggtcacc gtgaagatgc acctctcgaa atggtcgttg tcgtcgtagc cggtgcgcca
     3061 ctcgacggtg gtggtcccga acgggaagac tgatccgggc gcataccctt ccaccaggta
     3121 ggtcgccacc gggccgcacg agtcggatgc ggtgggtgcc gtgtaattga tgacggcgcc
     3181 gtgcgtaccg cactcggtca cgacgatgtc gtcgacgcag gtgatctggc gatccccgca
     3241 ctgggcgtaa acggacgatg gaacgacgag cgtcaatgcg agtgccgcaa tgacgcgtgc
     3301 ggtgtcggcg aagcagaaca gtggacgcat gattcctccg gatcggggtt acttgatgag
     3361 aaggagccgc aggacgcgcg agtcgttacc ggcgcgcagc cgggcaaagt agaggccgga
     3421 agggagctcg gtaccggcgt cgtcccggcc gtcccaggaa cgatcgaacc ggcccggctc
     3481 cagttcgcca tcgatgaggc gccggacgag gcgaccggcg ggatcgatga tcgtcaccga
     3541 caccgggccc ttcgccgcca cgctgaagcc gatcgacgcc acgccggaaa agggattggg
     3601 gccggcgctc cagagctgcg tgacggtcgg cgccgcctcg gcgctcacgc ccgactgcgc
     3661 gatggcctgc tcggtggcga agggcgatcc ttcgtacgcg gcgtcgatgg cctgcacgct
     3721 ccagtaccat ggcccgccgc cgggcaggtc gatcgtgcag ttggttcgct ggcctgtgtt
     3781 gccgcgggcc gcgatccggc gcaggccgct cgtcggcatc gccatcggcg acacgatctc
     3841 gctcccgccc gacgtggaac cgacgcggat gttgtacgtg aacgccgcgg ccggcgtatt
     3901 gttgtccacc gccgcgttcc acgaaaacgt ggcccggttt ccgaccatgg ataccgacag
     3961 gttcgagggc ggctgcggtg gcgtgttcaa cggccggcag gtgttccgca tcaccttcgt
     4021 ggcgtacggc gctcccggca ggcccgacag cacgatgtcg aggtcgtggt ccttgtccat
     4081 gtcgccgaaa gccgcggagc cgtagtacac gccgggcaat ggatccgccg aaacggtgaa
     4141 gacagggttc gcgccaccgc cgttatcgta gagccgcgca ctggagccgc tgccgtcgtt
     4201 gcccgcgatc aggatgtcga ggtcgccgtc gttgtcgtgg tcgccgaacg ccgccgatcc
     4261 ggaaacgact cccgtgaagc tggcgatggg cgtgccgaac gtcggggtgg ggccgcccgg
     4321 gttgcgccag agccgtgcca ccttcaccgg gccggtcgct tcgccgcaga cgacgagatc
     4381 ggggtcgccg tcggcgtcgt aatcccccca ggcgtggctt ccgttgatga cccccgtgat
     4441 gccggtgggc actccggcga aggtcgggtt cgccccgccc gaactccggt agagcatcgc
     4501 ggtcgtgctc gagcctccgc ctcccgcggt gcggcctgtc atcaggatgt cgaggtcacc
     4561 gtccacatcg aagtcgcaga gcgatacggc gctgtaggtg aggccgatga gtccggccgc
     4621 ggcatccgtg aagacgggat cgacaccgcc cgagctccgg aaaatccgtg agatgccgat
     4681 cgtgccgtcg tttccggtca cgaccaggtc gaggtcgccg tcgttgtcga tgtcgcccca
     4741 ggccagcgag ccgaagaacg tgccgggaag gccggcgccg atgtcgacga agaggccgaa
     4801 ggactgccgg tacacgcggg tgatgggtcc cgagccggta ttccccgaca gcgccaggtc
     4861 gagatcgccg tcgttgtcca tgtcgcccca cgcggcggcg ccgtacacgg tgcccgggag
     4921 aacggcgttc acggtgttgt agaggggctg gccgaagacg ccgtcgtacc ggtacacctc
     4981 ggtaaagtag gtcgacccat cgggcgtgcc gctgacgacg aggtcgaggt cgccgtcgtt
     5041 gtcgtagtca ccccaggccg acgcgccacc ggccgtgccg gtcacaccgc tcgccacgtc
     5101 ggcgaacccg gccacgcaga gcgactgctc ggtggcgaac ggcgagccga tgaacgcgcc
     5161 atcgatggcc tgcacgctcc agtagacggg tccgggaaac ggcagctcga tcgtccacgt
     5221 gaggttctgc atggcattgc cgagcgcggg aaccgtgcgg cgccccgtgg ccagcgccat
     5281 cggcg
//
LOCUS       DASVOD010000588         7235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_195254, whole genome shotgun sequence.
ACCESSION   DASVOD010000588 DASVOD010000000
VERSION     DASVOD010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195254"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..803)
                     /locus_tag="VF720_12970"
     CDS             complement(219..803)
                     /locus_tag="VF720_12970"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7880319.1"
                     /translation="MKRILTGLMMVAALSITTAPGAEAAHPGFGLGVHGGYGESDGAD
                     SGSPLAGIHLTLNVTPWLGIVAMGDYKFEEDVTQFDQDFTIKSYPISGMARLYLPIES
                     FSPYVAAGVQYRLISYGGDAFDDVEIDDSDDGFGWLAGAGAEFNLGTNKGLFVEGRFE
                     SFDPEQDVDNALDDLEELSYDQWAAIAGLTFYFK"
     gene            complement(1030..1608)
                     /locus_tag="VF720_12975"
     CDS             complement(1030..1608)
                     /locus_tag="VF720_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880320.1"
                     /translation="MKPSNRTTRTSPRITAALLLVATLGLSSCGGTGFQQTFVEPTAV
                     GVALKKVAVVGAANDTTLRRSFESRFARTLDLRGNDARAGYEFITEPMRTDLNSIKNK
                     LVEENFDGVLVTRLGPSTTDITRSFEGNLASPGPASEGDLQLRTSLYRVKDGRLLWDA
                     VTKSARGGNPDDAIASFSETVARELGAQGILR"
     gene            1696..1905
                     /locus_tag="VF720_12980"
     CDS             1696..1905
                     /locus_tag="VF720_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880321.1"
                     /translation="MNLRAVHIAAFVVVILGRIVFAILFLTQKKPGHEKAARIAPAWW
                     RAIASSRPAPAGWSDTPSTRRCPAS"
     gene            complement(1942..2202)
                     /locus_tag="VF720_12985"
     CDS             complement(1942..2202)
                     /locus_tag="VF720_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family protein"
                     /protein_id="HEX7880322.1"
                     /translation="MSQPGNPLHGITLERMVTELVAVHGWEELGRRIRIRCFTSDPSV
                     ASSLSFLRKNPWAREKVENLYRRTVLPRPRPRPGTIAPTRRE"
     gene            2310..3725
                     /locus_tag="VF720_12990"
     CDS             2310..3725
                     /locus_tag="VF720_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880323.1"
                     /translation="MTEPEIARKVAAHRLLATAPADEVAWVLAHGTLRTFAPGQIMTR
                     RSAENVEGIYFVLAGHLTISVDRGAGPHKVLEWRGGDTAGILPYSRMTTPPGDVTAEA
                     PTEVFLIRRQDFPEMIRSCHEIISKLVHVMVDRARQFTTSDLHDEKMVSLGRLAAGLA
                     HELNNPASALARGAKLLPERLVSSEAASIALGAAGLSEGDRAAIDEIRDLCACTPVNR
                     VRSPLEDADRESTLADWLKDHGADEALAEPLAESAATLEALDRLASVLDGAKLEAALQ
                     WVAAGCATRALARELEEAAVRISDLVGSVKGFTQMDQVGITEPIDIGLGLRQTLAVIQ
                     AKARGKSISVEVVAEPNLPRVRGVAGELNQVWLNLIDNALDAAPESGRVTIRADHADG
                     KVRVSVIDNGGGIPPEIQKRIFEPFFTTKGVGQGPGLGLGLSRRLVRKHIGEMAVESA
                     PGRTEFRVTLPVLADTEAGGP"
     gene            3722..5383
                     /locus_tag="VF720_12995"
     CDS             3722..5383
                     /locus_tag="VF720_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7880324.1"
                     /translation="MSKPVILAVDDDPQVLAAIRRDLREHYRSDYSVIAARSGEEALE
                     AVRQLKARGDELALVLSDQRMPGLSGVEVLAACREIYRLARRVLLTAYSDIGAAVRAI
                     NEAHLDYYLSKPWDPPEERLYPVIDDLLDAWQVERVPAQVGLRLVGDQWSPRSHAIKN
                     FLGANLIPYRWIDYGRDPDAQALCEATGVTREQLPALFLEDGSVLRNPDIVEIARTLQ
                     LSTEAAHELYDLAIVGAGPAGLAAAVYGASEGLRTVILDAHAPGGQAGTSARIENYLG
                     FPAGVSGSELARRAVTQAQRLGAEVLAPIRVESVTVEAGYKRLTLGDGRVVVARALLA
                     TTGMVYRDHPADGITTLSGAGVYHGASTTEGTALAGRRVMVVGGGNSAGQAALYLSRF
                     AGHVDIVIRRDSLKPTMSQYLIDQIDASPRIDVRAMTVIERVEGEGRLERVHLASSDN
                     GTHTVEEVDAVFIFIGTRPQSDWLPGEVLRDSKGFVVTGRDLAACAELPRLWKEQREP
                     FLLETSVPGLFAAGDVRAGAMNRVASAVGEGSMAVRMVHAYLATT"
     gene            complement(5418..5879)
                     /locus_tag="VF720_13000"
     CDS             complement(5418..5879)
                     /locus_tag="VF720_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7880325.1"
                     /translation="MNPVNAAGDIRVGPVVVTFRLEAEQTGGGLTMFEFTVPPKARVP
                     VPHSHDAFDETVYGLDGVMTWTLDGKAVSVGPGDVLFIPRGHVHGFENQGDVASRMLS
                     VITPGILGPTYFRDVADVLNAGGPPDVARIMEVMRKHGLKPVAPGAPPAGA"
     gene            complement(5894..6694)
                     /locus_tag="VF720_13005"
     CDS             complement(5894..6694)
                     /locus_tag="VF720_13005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7880326.1"
                     /translation="MDPIAQFKDNQRKAWSTFAPTEFYTCRPAARLAVFARIGEGQKV
                     LDVGCGTGVVSLAAARMGAAVTGLDLTPELLVRARENAAIAGADIAWQEGDAEALPFA
                     DGSFDVVVSQFGHMFAPRPAVVTAEMLRVLRPGGTIAFATWPPELWTGRIFMLVGRYM
                     PPLPEGVSPPVQWGDPALVRERLGAGVTDITFDRGVMASPYLSAQHVRIFMEQNVGPV
                     MKVAQMLAGEPDRLATFRGELDALSALYFEPTENVLRQDYLLTRATRA"
     gene            6833..>7235
                     /locus_tag="VF720_13010"
     CDS             6833..>7235
                     /locus_tag="VF720_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7880327.1"
                     /translation="MRIPILIQGGMGIGVSDWRLARGVAGRGQLGVVSGTLIDTLLVR
                     RLQQGDAGGHLRRAMLRFPIPGVANEVLRRYFVEGGVGEELPFQLLPMWRQRTTLFQE
                     KLAALAAFVEVWLAREGHQGPVGINLLSKIPI"
BASE COUNT         1162 a         2531 c         2398 g         1144 t
ORIGIN      
        1 cgcccgcatc ggcgtgcgtt gcagcccggc cgccctgcag ggggagtatg ccctcaccgc
       61 caccggattc gcggggctct cggcctgctc cgccgtcaag atgcgcgcgg ggttcgccgt
      121 cgtgatggag taacggggcc ccgtgacgaa cagaacaaaa aagtccggaa gcggcatcga
      181 accgcatccg gacttttttt ccgatttcga gcgggctgtt acttgaagta gaacgtcagc
      241 cccgcgatcg ccgcccactg gtcgtagctc agctcctcga ggtcgtcgag ggcgttgtcc
      301 acgtcctgct cgggatcgaa cgactcgaag cgcccctcga cgaagagtcc cttgttcgtg
      361 ccgaggttga actcggcgcc ggcgccggcc agccagccga agccatcatc gctgtcgtcg
      421 atctccacgt cgtcgaaggc gtcgccgccg tagctgatca gccggtactg cacgccggcc
      481 gccacgtacg ggctgaagct ctcgatcggc aggtagagac gggccatgcc actgatggga
      541 tagctcttga tggtgaagtc ctggtcgaac tgcgtgacgt cttcctcgaa cttgtaatcg
      601 cccatggcca cgatcccgag ccagggagtg acgttcagcg tgaggtggat accggccagc
      661 gggctgccgc tgtcggcgcc gtccgactca ccgtagccgc catggacacc gagaccaaat
      721 cctgggtgtg cggcctccgc gccgggcgcc gtggtgatcg aaagcgcggc caccatcatg
      781 agacccgtca ggattcgttt catggttcga ctcctccgga accttgtatc cgcatcgcga
      841 gcgcgaagcg ggcagccgag attcatcggc cggtcgcacg agcaacctcc tgatactaag
      901 ccaacgcggc cccctggcaa gtgcaaccct ccgaccgctc tcggggctgg cgctgcgcga
      961 taacccaccc ggtgccgttg atcgagatgc tctggtgggt caccggccac cggatcccct
     1021 gatccgcgcc tagcgcagga tcccctgggc gccgagctca cgcgccacgg tttcgctgaa
     1081 cgacgcgatg gcgtcgtccg ggttcccgcc gcgggcgctc ttcgtgacgg catcccagag
     1141 caatcggccg tccttcaccc ggtaaagcga cgtgcgaagc tgcaagtctc cctcgctcgc
     1201 cggccccggc gaggccaggt tcccctcgaa ggaacgggtg atgtcggtgg tggacggtcc
     1261 caaccgcgtc accagcacgc cgtcgaaatt ctcttcgacg agcttgttct tgatgctgtt
     1321 gaggtcggtg cgcatcggct cggtgatgaa ctcgtagccg gcacgcgcat cgttcccacg
     1381 caggtcgagc gtgcgcgcga atcgactctc gaaacttcgc cgcagcgtcg tatcgttcgc
     1441 cgccccgacg accgccactt tcttgagcgc aaccccgacc gcggtcggct ccacgaaggt
     1501 ctgctggaat ccggtcccgc cacacgagga gaggccgagg gtggccacga ggagcagggc
     1561 cgcggtgatc cggggtgacg tgcgggtcgt ccggttcgat ggtttcatga gcggaatttc
     1621 agggaatcgg gcgggagtgt caaccggggg ccaccccgcg ttgtcccacc gacttcgcgg
     1681 gagtaggctc gccccatgaa cctgcgagcc gtccatattg ccgcgttcgt cgtggtcatc
     1741 cttggacgga tcgtcttcgc catcctcttc ctcacgcaga agaagccggg ccacgagaag
     1801 gccgcgcgca tcgcgcccgc ctggtggagg gccatcgcct cgtcacggcc ggcccctgcc
     1861 ggctggtccg acaccccatc tacacggcga tgcccggcaa gctgatcgcc accgcgagca
     1921 gttcgggccg gagtacgagg attactcgcg gcgtgtcggt gcgatcgtgc cgggcctggg
     1981 cctgggcctg ggaagcaccg tgcgccgata gagattttcc accttctcgc gcgcccaggg
     2041 attctttcgg aggaagctca ggctcgaggc cacgctcgga tcactcgtga agcagcggat
     2101 ccggatgcgc cgccccagtt cctcccagcc atgcaccgcc acgagttcgg tgaccatgcg
     2161 ttccagggtg attccatgca gcggattgcc gggctggctc atcgcgcaag actacacgtt
     2221 cggcggccac tggccggctt ccggtttctt gccacgggcc gcccagcggg atacgcttcc
     2281 ccgccctgca gaggacgaag cgaaccgaca tgaccgaacc cgaaatcgcc cggaaagttg
     2341 ccgcgcaccg actccttgcc accgccccgg ccgacgaagt ggcctgggtg ctcgcgcatg
     2401 gcacgctgcg cacgtttgcc cccggccaga tcatgacccg ccggtcggcg gagaatgtcg
     2461 aagggatcta tttcgtcctt gccgggcacc tcaccatcag cgtggatcgc ggggcggggc
     2521 cgcacaaggt gctggaatgg cggggcggcg acaccgccgg gatcctcccc tactcgcgca
     2581 tgacgacacc tcccggcgac gtgaccgcgg aggcgccgac cgaagtcttc ctgatccgcc
     2641 gccaggattt ccctgagatg atccgctcgt gccacgagat catctccaag ctcgtgcatg
     2701 tgatggtcga tcgcgcccgc cagttcacca cgagcgatct ccatgacgag aagatggttt
     2761 cgctcggacg gctggccgcc ggcctcgcgc atgaactgaa caacccggcc tcggccctgg
     2821 cgcgcggcgc caagctcctg cccgagcgtc tcgtctcctc ggaggccgcg tccatcgcac
     2881 tgggcgcggc cggtctcagc gagggcgatc gcgccgccat cgatgaaatc cgcgatctct
     2941 gcgcctgcac gccggtcaac cgggtgcgct ccccgctgga agatgccgat cgcgagagca
     3001 ccctggccga ctggctcaag gatcatggcg cggatgaagc gctcgccgag ccgctggccg
     3061 aaagcgccgc caccctggaa gcgctcgacc ggctcgcgag cgtgctcgac ggcgcgaagc
     3121 tcgaggcagc ccttcaatgg gtggccgccg gatgcgccac gcgcgcgctg gcccgggaac
     3181 tcgaggaggc cgcggtgcgc atctccgacc tcgtcggctc ggtcaaggga ttcacgcaga
     3241 tggaccaggt cggcatcacc gagccgatcg acatcggcct ggggctccgg cagacgctgg
     3301 ccgtcatcca ggccaaggcc cgcggcaagt cgatcagcgt ggaagtcgtg gccgaaccga
     3361 acctcccccg cgtgcggggc gtggcgggtg agctcaacca ggtgtggctc aacctcatcg
     3421 acaacgcgct cgacgcggct cccgaatcgg ggcgggtcac gatccgcgcg gatcatgccg
     3481 acggcaaggt ccgtgtcagc gtgatcgaca acggcggcgg gattccgccc gagatccaga
     3541 agcgcatctt cgagccgttc ttcacgacca agggagtcgg acagggaccc gggctcggcc
     3601 tcggcctctc ccgccggctc gtgcgcaagc acatcggcga gatggcggtg gagtcggcgc
     3661 ccggccgcac ggagttccgg gtgacactgc cggtcctcgc cgacaccgag gcgggtggcc
     3721 cgtgagcaag ccggtcatcc tggcggtgga tgacgacccg caggtgctgg cggccatccg
     3781 ccgcgacctg cgggagcact atcgctccga ctacagcgtg atcgcggccc gctcggggga
     3841 agaggcgctg gaggccgtgc ggcagctcaa ggcccgcggc gacgaactgg ccctcgtcct
     3901 cagcgaccag cggatgcccg gcctgtccgg cgtggaggtg ctggccgcct gtcgcgagat
     3961 ctatcgactg gccaggcgcg ttctgctgac ggcctactcc gacatcggcg cggcggtgcg
     4021 cgccattaac gaggcgcacc tcgactacta cctgtcgaag ccgtgggatc cgccggagga
     4081 acgcctctac cccgtcatcg atgacctcct cgacgcctgg caggtggaac gcgtgccggc
     4141 tcaggtgggc ctgcgcctcg tcggcgacca gtggtcgccc cgttcccacg ccatcaagaa
     4201 tttcctgggg gccaacctca tcccgtaccg gtggatcgac tacgggcgcg atccggacgc
     4261 gcaggccctg tgcgaggcca cgggcgtcac gcgcgagcag ctccccgctc tcttcctcga
     4321 ggatggatcg gtgctgcgca atccggacat cgtcgagatc gcccgcacgc tgcagctctc
     4381 caccgaagcc gcgcatgaac tctacgacct cgcgatcgtg ggggccggac cggccggcct
     4441 cgcggccgcc gtctatggcg cctcggaggg gctgcgcacg gtcatcctcg atgcccatgc
     4501 gccgggagga caggcgggaa cgagcgcgcg gatcgagaac tacctgggat ttccggcggg
     4561 cgtcagcggc agcgagctgg cccggcgcgc cgttacgcag gcccagcgcc tcggcgccga
     4621 agtcctggcc cccatccgcg tggagagcgt gacggtcgag gccggctaca agcggctcac
     4681 gctcggcgac ggccgcgtgg tggtggcgcg cgccctcctc gccaccaccg gcatggtgta
     4741 tcgcgatcat cccgccgacg ggatcaccac gctcagcggc gccggcgtgt atcacggcgc
     4801 ctccacgacc gaagggacgg ccctcgcggg gcgccgtgtc atggtcgtcg gcggaggaaa
     4861 ctcggccggc caggccgcgc tctacctttc ccgtttcgcc ggccatgtcg acatcgtgat
     4921 ccggcgcgac tcgctgaagc ccaccatgtc gcaatatctc atcgaccaga tcgacgcctc
     4981 cccccgcatc gacgtgcgcg ccatgaccgt catcgagcgc gtggagggag agggccgcct
     5041 ggaacgcgtg cacctggcct cgtccgacaa cggcacgcac acggtcgagg aggtcgatgc
     5101 cgtcttcatc ttcatcggca cccgccccca gagcgactgg ctcccgggcg aggtgctgcg
     5161 cgactcgaag ggattcgtcg tgaccggccg cgacctggcc gcctgcgcgg agctaccacg
     5221 tttgtggaag gaacagcggg agcccttcct cctggaaacg agcgtccccg gcctcttcgc
     5281 cgccggcgac gtgcgcgcgg gcgccatgaa ccgggtggcc tcggccgtcg gcgaaggctc
     5341 gatggccgtc cggatggtgc acgcctacct ggccacgacc tgaggccgca gaggcccgcc
     5401 gagcgatgcg tgggttgtca ggcccccgct ggcggcgctc cgggcgcaac cggcttcagc
     5461 ccgtgtttcc gcatcacttc catgattcgc gccacatccg gcggtccacc ggcgttcagg
     5521 acatcggcca cgtctcggaa gtaggttggc ccgagaatcc cgggagtgat gacgctcaac
     5581 atgcgcgacg ccacgtctcc ctggttctcg aatccgtgca cgtggccgcg cgggatgaag
     5641 agaacgtcac ccggcccgac ggaaacggcc ttgccgtcga gtgtccaggt catgacgcca
     5701 tccaggccgt agacggtctc atcgaaggcg tcatgactgt gcgggactgg cacacgggcc
     5761 ttcggcggca cggtgaactc gaacatcgtc agcccgccgc ccgtctgttc ggcctcgagg
     5821 cgaaaggtca cgaccacggg gcccacgcga atgtcaccgg cggcattgac tggattcatg
     5881 gcgggctccc ccttcaggcc ctggtcgcgc gcgtcagcag gtaatcctgc cggaggacat
     5941 tttcggtcgg ttcgaagtag agcgcactca gggcgtcgag ctcaccgcgg aaggtggcca
     6001 gcctgtccgg ctcgccggcg agcatctggg ccaccttcat gaccggtccc acgttctgtt
     6061 ccatgaagat gcggacatgc tgcgcgctca ggtacggaga ggccatgaca ccgcgatcga
     6121 acgtgatgtc ggtaacgccg gcacccaggc gttcgcgcac gagcgccgga tcgccccact
     6181 gcaccggcgg tgacactccc tccggaaggg gcggcatgta gcgcccgacc agcatgaaga
     6241 tccggccggt ccagagctcg ggaggccagg tggcaaaggc gatcgtgccg cccgggcgca
     6301 gcacacgcag catctcggcc gtgaccaccg cgggacgggg cgcgaacatg tggccgaact
     6361 ggctcaccac cacgtcgaaa ctcccgtccg cgaacggcaa cgcctccgca tccccctcct
     6421 gccacgcgat gtcggcgccc gcaatcgcgg cgttctcccg cgcgcgcacc aggagctcag
     6481 gcgtcaggtc gagaccggtc accgcggcac ccatccgtgc cgccgccagc gacaccacgc
     6541 ccgtcccgca accgacatcg agcaccttct gcccttcgcc aatccgggcg aacacggcca
     6601 gccgcgccgc ggggcggcag gtatagaact ccgtgggcgc gaacgtcgac caggctttac
     6661 gctggttgtc cttgaactgc gcgatggggt ccatcgggtg tctccttctg gtcgtcaaag
     6721 ggccgattca cacggcatga taaccgcaat caggctgcga attgtcagcc cccacgcatc
     6781 ctggcggcac cgaagaccat gaaccccgcc accgccgacg taccatcccc cggtgagaat
     6841 tccaatcctg atccaggggg gcatggggat cggcgtgtcc gactggcgcc tggcccgggg
     6901 cgtcgccggc cgcgggcagc tgggtgtggt gtccggcacg ctgatcgaca ccctcctcgt
     6961 ccggcggctc cagcagggcg atgcgggagg gcacctgagg cgggcgatgc tccggttccc
     7021 gatcccgggc gtggcgaacg aggtcctgcg ccgctacttc gtggaagggg gtgtcggcga
     7081 ggagttgccg ttccagctcc ttccgatgtg gcgacaacga acgactctgt tccaggagaa
     7141 actcgcggcg ctggcggcct tcgtggaagt ctggctggca cgcgaggggc accaggggcc
     7201 tgtggggatc aatctcctga gcaagatccc cattc
//
LOCUS       DASVOD010000589         5037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_559889, whole genome shotgun sequence.
ACCESSION   DASVOD010000589 DASVOD010000000
VERSION     DASVOD010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559889"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VF720_13015"
     CDS             complement(<1..396)
                     /locus_tag="VF720_13015"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HEX7880328.1"
                     /translation="MLVLKFGGTSVQDAAAIRRAAAIVAGCGCPQAVVVVSAMAGVTN
                     ALVEAGASARAGDLTSAHRILDTIVLRHHETAEALVDGAARGELAGAIERHRAELLDL
                     LTALFRAPDVAGRTMDALLAQGELLSSHLV"
     gene            complement(442..1137)
                     /locus_tag="VF720_13020"
     CDS             complement(442..1137)
                     /locus_tag="VF720_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880329.1"
                     /translation="MLLARASHVVRTILTAVTLAAVAPAIALAVPYTWAVPMGGETWG
                     TGHVHTLLWSGGPANPTNIYLIHVPSNTVAQVVALNTVNDGEVQFRLNSGLAPGPYLF
                     YIEDNIPTEWTYGPEIQIRETPACIAPCTQGSFGAPLTVCGQTQAEAESLAIALAQSH
                     ISCGMAGNVVPGSVSIETTLINVGGYPCPAPYGGAYAVEVSAVWCCCHEPTDVEGRTW
                     SGIKVLLRDDTEQ"
     gene            complement(1260..2354)
                     /locus_tag="VF720_13025"
     CDS             complement(1260..2354)
                     /locus_tag="VF720_13025"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX7880330.1"
                     /translation="MDPSAPAVDVYAYDPVAIEATRGDTSWRQAARQELAERPPGAGR
                     PFPVDSALTVTPGVRAGARPDSLPELTLQVWLDRMHFSPGVIDGKAGKNTAKALYWFQ
                     DAYGLKPTGDLDPDTREVLLRLAGQWEELARYTVTAADIDQPFTPLGRDMYAKAEQKC
                     LCYESLLEAVGEKFHTTPEFLLSLNPGLDSATVTAGTELNVPNVDAAWADGGGNADST
                     RVVDRIVVSRKGFYTHAVDADGNILYHFPSTLGSKYDPSPEGKLKIAGIAFKPTFHYQ
                     PKLFADVPDEKPEAHLPAGPNSPVGVVWMQLSKENYGIHGTATPQTIGYESSHGCIRL
                     TNWDATTLARHVKRGVRVDFTNQATATVAG"
     gene            complement(2548..3795)
                     /locus_tag="VF720_13030"
     CDS             complement(2548..3795)
                     /locus_tag="VF720_13030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880331.1"
                     /translation="MNPIDLFRMSTGALVGNKLRSTLTLVGIVLGVASIIAVMTALSV
                     VQGTMEQEMSVLGAQTFQVQKWPAGGFNSPEERRRAMRRPPLTVEDAEAIRRNVDSVD
                     LVGSELWDFGYAVEYKGESTNPNVSICGGTPEYAPNNTHFVGQGRNLSQMDIRSGRMV
                     AVIGFAIAGKLFPFVDPVGKIVRVDGRKYEVIGVFDEKKSAFGSNFDNYVLIPVTTFL
                     DIYGMKNRDGFDRSVNITVRAKTPELVPDAIARTRQVLRQARGVKPGEDDNFDFYNSE
                     SLIAEFNKMSAGVKLAAFAIGTIALIVAGIGIMNIMLVAVTERTKEIGIRKALGARSR
                     TILAQFLLEAVILCNMGGVVGVAIGFALGNVMTFFTKFQARVPLEWAVIGLLFCTAVG
                     VGFGMLPAIRASRLNPIDALRYE"
     gene            complement(3835..>5037)
                     /locus_tag="VF720_13035"
     CDS             complement(3835..>5037)
                     /locus_tag="VF720_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880332.1"
                     /translation="GIAGRAIRANKGRGGLTTLGIIIGIVAVVLTMTAVNGLQNRMKE
                     SFSAVGADVVYVSRMPWIIMNDFFVYRNRPQINMREAELLEERLRGKALVNPSLDTQR
                     DLKYRAETIEGVRVIGTTEKQTRLSAAQPQIGRFLTVSDVNYKKSVCVIGTEVRKALF
                     GSADPVNKVIRVGAVDFRVIGVMEKQGGSFLGGPNFDRQIYVPITTFVKSFGGQRRRD
                     VNIAVQAPSPDRLTELEFEVIGEMRKIRRLRPAEGDNFSINKLDTLVGTFGKMMSTVL
                     LVGLLVTGISLFVGAVGVMNIMFVSVTERTREIGIRKAIGATRRSILLQFLLESSAIS
                     LLGGGVGIVVAAILTAVLNATVMPASISPAIVMIATVTSIAVGILAGIVPAWRGARLD
                     PIESLRYE"
BASE COUNT          819 a         1683 c         1686 g          849 t
ORIGIN      
        1 caccaggtgc gaggacaaca gttcgccctg cgccagcagg gcgtccattg ttcttcctgc
       61 gacgtcgggc gcccgaaaca gcgcggtcaa caggtcaagc aattccgccc ggtgacgctc
      121 gatcgccccg gccaactcgc cgcgtgccgc gccatccacc agcgcctcgg cggtttcatg
      181 atgtcgcagg acgatcgtat cgagtattcg atgggcgctc gtcaggtcac ccgcgcgtgc
      241 ggacgccccg gcttcgacca gcgcgttggt cacgccggcc atggcagaca ccacgacgac
      301 tgcctgcggg cagccacacc ccgccacgat ggccgcggcg cgccggatgg cggcggcatc
      361 ctgcacggag gtgccgccga acttgaggac gagcatgcgg cattgtggac aagtcgggcg
      421 cgggagggaa gcgcctcccc gctactgctc cgtgtcgtcc cgcagcagga ccttgatccc
      481 cgaccaggta cgcccctcga catccgtcgg ctcgtggcag cagcaccaca cagccgacac
      541 ctccaccgcg taggcgccgc catacggcgc cgggcaggga tagccgccga cgttgatcag
      601 cgtggtctcg atgctgaccg atccgggcac gacgttgccg gccatgccgc acgaaatgtg
      661 gctctgtgcc agcgcgatgg cgagcgattc ggcttcggcc tgcgtctggc cgcacaccgt
      721 gagcggcgcg ccgaacgaac cctgcgtgca gggcgcgatg cacgccggcg tctcgcggat
      781 ctggatctcg ggaccgtagg tccactcggt cgggatgttg tcctcgatgt agaagaggta
      841 gggcccgggc gccaggccgg aattcaaccg gaactgaact tcaccatcgt tgacggtgtt
      901 gagcgccacg acctgcgcga cggtattgct cggcacatgg atgaggtaga tgttggtcgg
      961 gttggccggc ccaccgctcc agaggagcgt gtgaacgtgt ccggtgcccc aggtctcgcc
     1021 tcccatcggg accgcccagg tgtagggcac ggcgagggcg atggcgggag caacggcggc
     1081 aagagtcaca gcggtaagga ttgtccggac gacgtgggat gcgcgagcaa ggagcattcg
     1141 gacctccggc gcaatggggg aagcgaccga aagggatgcc gcatcttagc atgcgtcccg
     1201 gagttcccgg ccgccgccag cgggtggcct gtgtgctgcc ggcagtcccc cgccggccgt
     1261 cagccggcta ctgtcgccgt ggcctggttc gtgaagtcca cccgcacgcc gcgcttcaca
     1321 tgccgcgcca gcgtcgtcgc atcccagttc gtcaggcgga tgcaaccgtg tgacgactcg
     1381 tagccgatcg tctgcggcgt ggccgtgccg tggattccgt agttctcctt cgagagctgc
     1441 atccagacca cgcccaccgg cgagttgggg ccggccggca ggtgggcctc gggcttctcg
     1501 tcgggcacat cggcgaagag tttcggctgg tagtgaaagg ttggcttgaa ggcaatgccc
     1561 gcgatcttca gctttccctc cggcgacggg tcgtacttcg agccgagcgt ggaggggaag
     1621 tgatagagga tgttgccgtc ggcatccacg gcgtgcgtgt agaacccctt tcgggaaacg
     1681 acgatccggt cgaccacgcg cgtcgagtcg gcgttgccgc caccgtcggc ccatgcggca
     1741 tcgacgttgg gcacgttcag ctcggttccg gccgtcacgg tcgcggagtc gaggccggga
     1801 ttcagcgaca acaggaattc gggcgtggtg tggaacttct cccccaccgc ctcgaggagc
     1861 gattcgtagc agaggcactt ctgttcggcc ttggcgtaca tgtcccggcc gagcggcgtg
     1921 aacggctggt cgatgtcggc ggccgtcacg gtgtagcgcg ccagctcttc ccactggccg
     1981 gcgagccgca gcagcacttc gcgcgtgtcc gggtcgagat ccccggtcgg cttcaggcca
     2041 taggcgtcct gaaaccagta gagtgccttg gccgtgttct ttcccgcctt gccgtcgatg
     2101 acccccgggg agaaatgcat gcggtcgagc cacacctgca gcgtgagttc cggcaacgaa
     2161 tccggccggg cgccggcccg cactccgggc gtgacggtca gtgccgagtc caccgggaac
     2221 ggccggcccg ctccgggggg gcgctcggcc agttcctgcc gcgccgcctg tcgccacgag
     2281 gtatcgccgc gcgtggcctc gatcgccacc gggtcgtagg catatacatc cacggccggg
     2341 gcgctggggt ccatggcgag ggtgtcgcca tcgcctcgcg ggggcggctg tgcggccgtt
     2401 cgcgtctcct gcttcgaaca gccggccagg acgaggaggg tcagaccgag taaaagtgtc
     2461 gccaggcgtc tcatggagaa cggtggaaag caagagccgt tccactcctg ggaagcctgg
     2521 cccggctact cgaatccggg cccgccgcta ctcgtaacgc agcgcgtcga tgggattcag
     2581 ccgggaggcc cggatggccg ggagcatccc gaaaccaacc cccaccgcgg tgcagaacag
     2641 gaggccgatg acggcccact cgagcggcac gcgcgcctgg aatttcgtga agaaggtcat
     2701 cacgtttccc agcgcgaaac cgatggccac gcccaccacg ccccccatgt tgcacaggat
     2761 gacggcctcg agaaggaact gggccaggat ggtgcgcgag cgcgcaccca gggccttccg
     2821 gatgccgatc tccttggtgc gctcggtgac ggccaccagc atgatgttca tgatgccgat
     2881 gccggccacg atgagggcga tggtgccgat ggcgaaggcg gcgagcttca cgccggcgct
     2941 catcttgttg aattcggcga tcaggctctc gctgttgtag aagtcgaagt tgtcgtcctc
     3001 gccgggcttg acgccgcgcg cctggcgcag cacctggcgc gtccgggcga tggcgtccgg
     3061 caccagctcc ggcgtcttgg cgcgcacggt gatgttgacc gagcggtcga agccgtcccg
     3121 gttcttcatc ccgtagatgt cgaggaacgt cgtgaccggg atcagcacgt agttgtcgaa
     3181 gttgctgccg aaggccgact tcttctcgtc gaaaaccccg atcacctcgt acttccggcc
     3241 atccacgcgc acgatcttgc cgacgggatc gacgaacggg aagagtttcc cggcgatggc
     3301 gaacccgatc acggccacca tcctcccgct ccttatgtcc atctgggaga ggttccgccc
     3361 ctgtcccacg aagtgcgtgt tgttcggcgc gtactcgggc gtgccgccgc agatcgacac
     3421 gttgggattg gtcgactctc ccttgtactc caccgcgtag ccgaagtccc agagctccga
     3481 gcccacgagg tccaccgaat cgacgttccg ccggatggct tcggcatcct ccacggtgag
     3541 cggcggtcgg cgcatggccc gccggcgctc ctccggcgag ttgaatccgc cggccggcca
     3601 cttctgcacc tggaacgtct gggccccgag gacgctcatc tcctgctcca tcgtcccctg
     3661 cacgacggag agcgccgtca tcacggcgat gatcgaggcc acgccgagca cgatgccgac
     3721 cagcgtcagc gtggagcgga gcttgttgcc gacgagtgcg ccggtggaca tccggaagag
     3781 gtcgatcgga ttcatgacgg tgcctccccc ggcgtgacgc cggcgcccgg tgaactactc
     3841 atagcgcagc gactcgatcg gatcgagccg cgctccccgc caggccggaa cgatgcccgc
     3901 caggatgccg acggcgatgg acgtgaccgt cgcgatcatc acgattgccg gcgagatgct
     3961 ggcgggcatg acggtggcgt tcagcacggc ggtcaggatg gccgccacca cgatccccac
     4021 gcctcccccc aggaggctga tggccgacga ttcgagcagg aactgcagga ggatgctccg
     4081 cctcgtggcc ccgatggcct tgcggatgcc gatctcgcgg gttcgttcgg tgaccgacac
     4141 gaacatgatg ttcatgacgc cgaccgctcc cacgaacaac gagatgccgg tgaccaggag
     4201 cccgacgagg agcacggtac tcatcatctt gccgaaggtg ccgaccaggg tgtcgagctt
     4261 gttgatcgag aagttgtccc cctccgcggg gcggaggcgg cggatcttcc gcatctcccc
     4321 gattacctcg aactccagct cggtcaggcg gtcggggctc ggcgcctgca ccgcaatgtt
     4381 cacgtcgcgg cgccgctggc cgccgaacga cttcacgaag gtcgtgatcg gcacgtagat
     4441 ctgccggtcg aagttggggc cgccgaggaa gctccccccc tgcttctcca tcaccccgat
     4501 gacccggaag tcgaccgccc ccacgcggat caccttgttg acgggatcgg ccgaaccgaa
     4561 cagcgccttc cggacttcgg ttccgatgac gcagacgctc ttcttgtagt tcacgtcgga
     4621 gaccgtcaga aaacgcccga tctgcggctg ggccgccgag aggcgggtct gcttctcggt
     4681 ggtgccgatg acgcgaaccc cctcgatggt ttccgcgcgg tatttcaggt cgcgctgggt
     4741 gtcgagcgag ggattgacca gcgccttgcc gcgaagccgc tcctcgagca gttcggcctc
     4801 gcgcatgttg atctgcggcc ggttccggta gacgaagaag tcgttcatga tgatccaggg
     4861 catgcgcgac acgtagacga catcggcgcc caccgcggaa aagctctcct tcatccggtt
     4921 ctggagcccg ttcacggccg tcatggtgag gacgacggcc acgatcccga tgatgatgcc
     4981 gagcgtggtg aggccgcccc gccccttgtt ggcccggatg gcccgcccgg cgatccc
//
LOCUS       DASVOD010000590         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_402697, whole genome shotgun sequence.
ACCESSION   DASVOD010000590 DASVOD010000000
VERSION     DASVOD010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402697"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VF720_13040"
     CDS             <1..1774
                     /locus_tag="VF720_13040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX7880333.1"
                     /translation="TTVIERSAIESSGARNLGEILRPVVGVRVATTGGLGSFTPISIR
                     GSSNDQVLVLVDGRRLNAAQGGGVDLSDIPLDDIERIEVVRGGASAIHGSDAIGGTVN
                     LVTRSAETGGEAGARLEYGTNDTKLATLHLANGRDDAAWRLDVRRCLSDGWLRVAENG
                     SPVTGTDDSDGGRDDLDMIWGSGRITWRPAWASRIDLSGTHESSDKGVPGSTEFSSPF
                     ARQEDEFSSAVIELGGLFAGASAASGARVYAQRRLRGYEDPEYPLGAIDDQHRNDAWG
                     ASFDQDRDLGRGGRLKAGVEWRREELMSTTDGHHLRSSAAAWVRDAIALDDAGDVELT
                     PALRWDGVSDFDGRVSPMLMGRWTVSPTVTWRASAGGAFRPPAFDDLFQPPRASAAGN
                     PDLKPEHSWNGDLGVTLTPGAWSFGVTGFVNRVHDLIQWQPGAAGVWRPHNVDGARII
                     GAEVELAGPIGLPGSARVVNLSANYTALDPRETGEASNTAGKVLIYRPRHRLNAQARL
                     PLGRWEIDTQWGYTGPVYTTRANTKSLPGYVVGDAGVRWQVRAGLDVEGRVLNLTDEQ
                     YQDIRDYPAPGREWRLALVGRTGS"
     gene            1812..>2291
                     /locus_tag="VF720_13045"
     CDS             1812..>2291
                     /locus_tag="VF720_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880334.1"
                     /translation="MTIVPMGALLLATLLWGAEERLTTTTTDSETGLNHRTMAVDAFG
                     TLHVVWAEQNGPRGNYQVMTCRRPAGGNWSVPALVVPFRPEGIGNLLGAKFPSLVAGP
                     GDSLHLAWHDYRNGGILNCEIYVKSCAAGVPWDTSAATELRLTTSNHPETNGDNGYVP
                     "
BASE COUNT          353 a          746 c          850 g          342 t
ORIGIN      
        1 caccacggtc atcgagcgat cggcgatcga gtcgtccggc gcccggaacc tcggcgagat
       61 cctgcggccg gtggtgggcg tgcgcgtggc cacgaccggc ggcctcggtt cgttcacgcc
      121 catctcgatc cgcggttcgt cgaacgacca ggtgctggtg ctggtggatg gccggcggct
      181 caacgccgcg cagggaggtg gcgtcgacct gtccgacatc ccgctggatg acatcgagcg
      241 aatcgaggtc gtgcggggcg gcgcttcggc gatccatggt tccgacgcca tcggcgggac
      301 ggtgaacctg gtgacgcgtt cggcggagac cggaggcgag gccggcgcgc gcctggagta
      361 cggcacgaac gacacgaagc tcgccacgct ccacctggcc aacggtcggg acgacgcggc
      421 ctggcggctc gacgtgcgcc gttgcctctc ggatggctgg ttgcgcgtgg cggagaacgg
      481 ttcgcccgtg acgggcaccg acgattccga cgggggtcgc gacgacctcg acatgatctg
      541 ggggagcggc cggatcacct ggcgacccgc atgggcctcg cggatcgatc tttcgggaac
      601 gcacgagtcg agcgacaagg gagtgcccgg gagcaccgag ttctcctcgc cgttcgcgcg
      661 gcaggaggac gagttctcct cggcggtgat cgagttgggt ggactgttcg ccggggcgag
      721 cgcggcgtcg ggagcccgcg tgtacgccca gcggcggctg cgggggtacg aggatccgga
      781 gtatccgctc ggggcgatcg acgaccagca tcgcaacgac gcgtggggcg cctcgttcga
      841 ccaggatcgc gacctcggcc ggggcggtcg gctgaaggcg ggcgtcgagt ggcggcgcga
      901 ggagctgatg agtaccaccg atgggcatca tctccgctcc tccgcggcgg cctgggtgcg
      961 cgacgcaatc gcgctggacg atgcgggcga tgtcgagctg acaccggcgc ttcgttggga
     1021 tggggtgtcg gacttcgacg ggcgcgtgtc cccgatgctg atggggcgct ggactgtctc
     1081 gcccacagtc acatggcggg cttcggccgg cggcgcgttc cggccgcccg cgttcgatga
     1141 tctcttccag ccgccgcgcg cctcggccgc cggcaatccc gacctgaaac ccgaacactc
     1201 ctggaacggc gatctcggcg tgacgctgac gccgggtgcc tggtcgttcg gcgtgaccgg
     1261 cttcgtgaac cgggtacacg acctgatcca gtggcaaccg ggagcggcgg gagtgtggcg
     1321 cccgcacaac gtggatgggg cgcggatcat cggggccgag gtggaattgg cggggccgat
     1381 cgggctgccg ggaagcgcgc gggtcgtgaa cctgagtgcc aattacaccg cgctcgaccc
     1441 gcgtgagacc ggcgaggcca gcaataccgc cggcaaggtc ctgatctacc ggccgcgcca
     1501 ccggctgaat gcccaggccc gtcttccgct cgggcgttgg gagatcgaca cgcagtgggg
     1561 gtacaccggg cccgtctaca cgacacgcgc caacaccaag tcgcttcccg ggtacgtggt
     1621 gggcgatgcc ggcgtgcgct ggcaggtccg cgccgggctc gacgtcgagg ggcgcgtgtt
     1681 gaacctgacc gacgaacagt accaggacat ccgcgactac ccggcgcccg gccgcgagtg
     1741 gcggctggcc ctggtgggga ggaccgggtc atgacgggaa cgtttgcgct ccgaacgatc
     1801 aggaggggtg catgacgatc gtgccgatgg gagcgctcct cctggccacg ctcctctggg
     1861 gagccgagga acgcctgacg acgacgacga ctgattcgga aacaggtttg aaccaccgga
     1921 cgatggcggt ggacgccttc ggcacgctcc acgtcgtctg ggccgaacag aacgggccac
     1981 gcggcaacta ccaggtgatg acttgccggc ggccggccgg gggaaactgg agcgtaccgg
     2041 cactggtcgt gcccttccgc cccgagggaa tcggaaacct cctcggtgcc aagtttccgt
     2101 cgctcgtggc cgggccgggc gactcgctcc atctcgcgtg gcacgattac cggaacggcg
     2161 gcatcctgaa ttgcgaaatc tacgtcaagt cgtgcgccgc cggcgtgccc tgggacacca
     2221 gcgccgccac cgagctccgg ctgaccacct cgaatcaccc cgagaccaat ggcgacaacg
     2281 gctacgtgcc g
//
LOCUS       DASVOD010000591         2217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_873094, whole genome shotgun sequence.
ACCESSION   DASVOD010000591 DASVOD010000000
VERSION     DASVOD010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873094"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1225)
                     /locus_tag="VF720_13050"
     CDS             complement(128..1225)
                     /locus_tag="VF720_13050"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7880335.1"
                     /translation="MPDRRRAFDPRLGALVLALGLAFAACTKQGEPFMPARPGQVDST
                     KVEGSEWGHWTADNDPYYAIKSIVVPANRTLTIDPGVLVIFTRAGLTFTVKGTLIAPG
                     KPDSLINFRPNANRGVPPVAGDFGGVVFEGGSIGRLTGFRVQYGTTGIKATGSDLEMD
                     NCFISNHLQYGITAARTRLVLRNSTLSNCGLNGLDLNECDSPTYPVEIEHCNIGNNRY
                     AGIWAVNSSLVARRTDIRNNGDEGDPDFSAGIHFEGLPGIDPPIFRQCNINQNLPCDL
                     RNEMPTGIVVSADSNWWGPPTTDEMEFLSNPVVPPDPNKNNCSFNVRQICDGLDDPLG
                     GASGVRFCLWLDAPAPNFASSSMAPGAGSGR"
     gene            complement(1218..>2217)
                     /locus_tag="VF720_13055"
     CDS             complement(1218..>2217)
                     /locus_tag="VF720_13055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX7880336.1"
                     /translation="NLKLPEPERSGRIVLDVKDLGKSYGDLTVLDGISFRLQRGDKMG
                     VIGVNGAGKSTLLRLIVGQEAYDRGELRLGAGVEIGYFEQEQKALRGPHSVLDEAWKV
                     TPLVPENEIRTFLGGFLFSGEDVYKPLNALSGGERSRLALALIVRSRANFLVLDEPTN
                     HLDIPSRAVFEAAVEQFEGTVLTVSHDRWFLDRTVNQILAIDNGHAVLSLGNYSDYRR
                     RHAPVTSVPAVPAAAPPKPPTKTEPKTAANPSVSAPKVDKRARERWQKELARLEEAIA
                     VAEVDKSRIGAELESGAIIDRAQLAERGKAWQAAEDKLAALMAEWEKLQAREVTGA"
BASE COUNT          359 a          654 c          783 g          421 t
ORIGIN      
        1 ctgctcgaat cgggtgcttc atacaacggt tcaacactgg ttgctaaata caaagagccc
       61 ttcggcttat tagccgaagg gctccaagat caaactggcg ggccggacga gactcgaact
      121 tgaaccccta ccggcctgac cccgccccgg gtgccatgct tgaactcgca aaattagggg
      181 ccggcgcgtc cagccacagg cagaagcgca ctcccgatgc cccccccagc gggtcgtcca
      241 gcccgtcgca gatctggcgg acgttgaagc tgcagttgtt tttgttaggg tcgggcggga
      301 cgacggggtt ggagaggaat tccatttcgt cggtggtggg tgggccccac cagttgctgt
      361 cggcggaaac gacgatgccg gtgggcattt cgttccgcag gtcgcagggg aggttctggt
      421 tgatgttgca ctggcggaag atggggggat cgatgccggg gaggccttcg aagtggatgc
      481 cggcggagaa gtcgggatcc ccttcgtcgc cgttgttgcg gatgtcggtg cggcgggcca
      541 ccaaagacga gttgacggcc cagatgccgg cgtagcgatt gttgccgatg ttgcagtgct
      601 cgatctcgac cggataggtg ggtgaatcgc actcgttcag gtcgaggccg ttcaggccgc
      661 agttggagag ggtgctgttc ctgagcacga gccgggtgcg ggccgcggtg atgccgtact
      721 ggagatggtt ggagatgaag cagttgtcca tctcgaggtc ggagccggtg gccttgatgc
      781 ccgtcgtgcc gtactgcacg cggaagccgg tcagtcggcc gatcgatcca ccctcgaaca
      841 ccacgccgcc gaaatcgccc gccacgggtg gcacgccgcg attggcgttc ggacggaagt
      901 tgatgaggct gtccggcttg ccgggggcga tgagcgtgcc cttcacggtg aaggtgaggc
      961 cggcgcgggt gaagatgacc aggactccgg ggtcgatcgt gagggtgcga ttggccggca
     1021 cgactatcga cttgatggcg taatagggat cattgtcggc cgtccaatga ccccactccg
     1081 aaccctccac cttcgtggag tccacctggc cggggcgtgc cggcatgaac ggttctccct
     1141 gcttggtgca ggcggcgaac gccaggccga gcgccaggac gagcgcgccg aggcgcgggt
     1201 cgaatgcgcg gcggcgatca ggcaccggtc acctcgcggg cctggagttt ttcccactcg
     1261 gccatgagtg cggcgagctt gtcctcggcc gcctgccagg cctttccccg ctcggccagt
     1321 tgcgcgcgat cgatgatggc gcccgactcg agttccgcgc cgatgcggga cttgtcgacc
     1381 tccgcgaccg cgatggcctc ttccaggcgg gccagttcct tctgccagcg ctccctggcg
     1441 cgtttgtcga ccttgggtgc ggagacgctg gggttcgcgg ccgtcttggg ctctgttttg
     1501 gtgggcggct tcggaggcgc cgcggccggg actgctggta ccgaggtcac cggcgcgtgg
     1561 cgccggcggt aatcggaata gtttccgagc gagagcacgg cgtggccgtt gtcgatggcc
     1621 aggatctggt tgacggtgcg gtcgagaaac cagcggtcgt gtgacaccgt gagcacggtc
     1681 ccctcgaatt gttcgaccgc cgcctcgaac accgcccgcg agggaatgtc gaggtgattg
     1741 gtcggttcgt cgaggacgag gaagttggcg cggctccgga cgatgagggc cagggccagg
     1801 cggcttcgtt cgcctcccga cagggcattc aacggcttgt agacgtcttc cccggagaag
     1861 agaaaaccgc ccaggaacgt gcggatttcg ttttcaggga cgaggggcgt caccttccag
     1921 gcttcatcca ggaccgagtg ggggcctcgg agagctttct gctcctgttc aaagtagccg
     1981 atctcgacgc ccgcgccaag ccgcaattca ccccgatcgt acgcttcctg cccgacgatg
     2041 agccggagaa gggtggattt ccccgcgccg tttacaccga tgacacccat tttgtcgccg
     2101 cgctggaggc ggaacgaaat gccgtcgagc acggtgaggt cgccgtacga tttgccgaga
     2161 tccttgacgt ccaggacgat gcgcccggag cgttccggct cggggagctt gaggttc
//
LOCUS       DASVOD010000592         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_611739, whole genome shotgun sequence.
ACCESSION   DASVOD010000592 DASVOD010000000
VERSION     DASVOD010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611739"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VF720_13060"
     CDS             complement(<1..1068)
                     /locus_tag="VF720_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880337.1"
                     /translation="MPRAAGAPSGPPGRGGRWFGHVVLAFGLLALLLPVLSPGPDADV
                     WWHLRAGADFLAGRPGMFVESWSFTAAGTPWVNHEWLAEAFFALGERFGGDVGVILLA
                     TFLVLGTALLVARTALREKVPLPVVGALLAVAPLVMGDRLVPRPHLFSYFLLALTLER
                     FGAARRGEASWWWLPLIQLAWTNAHGPVVGLGVMTLLLLGGGTGWRARLPVLAAVLVA
                     SLVHPSGFRALTDYLAHLGGAGLYRQVVQEWQPILDPTQARLPGRLPTLVLAALTFLA
                     LCVSLIGGRPWPRWGLALLLAGLCAGPFLAARNRDFLAIAALPVLALLSGPWPSPRKG
                     WRVPIRTAGALITAMALVLVAV"
     gene            complement(1082..1405)
                     /locus_tag="VF720_13065"
     CDS             complement(1082..1405)
                     /locus_tag="VF720_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX7880338.1"
                     /translation="MIQASPRALQRIREIVREKGDPGLMLRVGVKGGGCSGFSYVLDF
                     TSERRPNDKVFEQDGVTILCDPKSYLYLSESLLDFGDGLLDGGFRFTNPHAKKSCACG
                     ESFSA"
     gene            complement(1402..2574)
                     /locus_tag="VF720_13070"
     CDS             complement(1402..2574)
                     /locus_tag="VF720_13070"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX7880339.1"
                     /translation="MTRPIYLDNNATTPIDPVVLEAMMPYLTGEFGNAASSSHVYGWQ
                     AAEAVETGRNQVARLIGGAPREIVFTSGATESNNLALKGAARAYREKGRHLVSCATEH
                     KAVLDTLKSLEAEGFEVTILPVNPDGLLDLDRLAAAIRPDTTVVSIMAANNEIGVLQP
                     LEAIGSLCRSREVVFHTDAVQAAGKIPVDVDRWQADLVSLTAHKLYGPKGVGALYVRQ
                     RDRKIRIAPQIDGGGHERGMRSGTLNVPGIVGLGKAFAVAKDSMAEESCRIEGWRNRL
                     WETIRTGITDVVLNGSLEHRLPGNLNVSIPNVEGESLVAALRDVAISSSSACSSASME
                     PSHVLKALGLSTPLAFRALRFGIGRFNTEAEITLVGELLVRKVTELRASSPLYSPS"
BASE COUNT          461 a          890 c          959 g          352 t
ORIGIN      
        1 caccgccacg aggacgaggg ccatcgccgt gataagagcg cccgcggtcc ggatgggcac
       61 ccgccacccc ttgcgcggcg acggccaggg gcccgacagg agagcgagta cggggagcgc
      121 cgcgatcgcc aggaagtcgc gattgcgggc ggcgaggaac ggtccggcgc acagcccggc
      181 gagcaggagg gcaaggcccc accgcggcca cggtcgccca ccgatcaacg atacgcacag
      241 ggccaggaac gtgagggccg cgagcacgag cgtcggcagc cgcccgggga gacgagcctg
      301 cgtcggatcg aggatcggtt gccattcctg caccacctgc ctgtagagcc cagcgccgcc
      361 gaggtgggca aggtagtcgg tgagcgcccg gaaccccgac ggatggacga gcgaggccac
      421 gagcacggcc gcaagcacgg ggagtcgtgc gcgccacccc gtaccccctc ccaggaggag
      481 cagcgtcatg acgccgagcc ccacgacggg cccgtgggcg ttggtccagg cgagctggat
      541 gaggggaagc caccaccacg aggcctcgcc gcgccgcgcg gccccgaagc gttcgagcgt
      601 gagcgccagc aggaagtagc tgaagaggtg ggggcggggg acgaggcgat cgcccatgac
      661 gagcggcgcc acggccagca gggcgcccac cacgggaagc ggcaccttct cccggagtgc
      721 cgtgcgggcc acgaggagcg cggtgccgag cacgaggaag gtcgccagga ggatgacgcc
      781 cacgtctccg ccgaaccgct cgccgagggc aaagaacgct tcggccagcc actcgtggtt
      841 cacccacggc gtgccggccg ccgtgaacga ccacgactcg acgaacatgc cggggcgacc
      901 ggccaggaag tcggcgccgg cccgcaggtg ccaccagaca tcggcgtcgg gaccggggga
      961 gagtacgggg aggagcaagg ccaggaggcc gaaggcgagg accacgtggc cgaaccaccg
     1021 gccgccccgc ccgggcggcc cggatggcgc cccggcggcc cggggcatca ggcgacgaac
     1081 gtcaggcgga gaagctctcg ccgcaggcgc acgacttctt ggcgtggggg ttggtgaagc
     1141 ggaagccgcc gtcgaggagc ccgtcgccga agtcgaggag gctctcggag aggtagaggt
     1201 aactcttcgg atcgcacagg atcgtcacgc catcctgctc gaataccttg tcgttgggac
     1261 gccgttcgga ggtgaaatcg aggacgtagg agaagcccga acagccgccc cccttgacgc
     1321 ccacccggag catcaacccg ggatctccct tttcgcggac gatctcccgg atgcgctgga
     1381 gagcccgtgg actggcctgg atcatgaggg agagtatagc ggactcgacg cccggagttc
     1441 cgtgaccttc cggaccagga gctcgccgac cagggtgatc tcggcctcgg tgttgaaccg
     1501 gccgataccg aaacggaggg cccgaaaggc cagcggcgtc gagagaccca gagccttgag
     1561 cacgtggctc ggctccatcg aggccgagga acaggccgag ctggaggaaa tcgccacgtc
     1621 gcgcagcgcc gccaccagcg attccccctc cacgttcggg atcgagacgt tcaggttgcc
     1681 gggaaggcga tgctcgaggc tcccgttcag gaccacgtcg gtgattcccg tccggatcgt
     1741 ctcccagagg cggttccgcc acccctcgat gcggcacgac tcctcggcca tcgaatcctt
     1801 tgcgacggcg aacgcctttc cgaggcccac gataccggga acgttcaacg tgccggagcg
     1861 catcccgcgc tcgtgcccgc cgccgtcgat ctgcggggcg atccggatct tccgatcgcg
     1921 ctggcgcacg tacagtgcgc cgacgccttt cggtccgtaa agtttgtgtg ccgtcagcga
     1981 cacgaggtcg gcctgccacc ggtccacgtc caccgggatc ttgcctgccg cctgcaccgc
     2041 gtccgtgtgg aagaccacct cgcgtgaacg acagagggag ccgatcgcct cgagcggttg
     2101 caggacgccg atttcgttgt tggcggccat gatcgacacg acggtggtgt ccgggcggat
     2161 cgccgcggcc aggcgatcga ggtcgagaag tccgtccgga ttcacgggaa ggatcgtcac
     2221 ttcgaagcct tcggcttcga gcgacttcag ggtgtcgagc accgccttgt gctcggtggc
     2281 gcagctcacc aggtggcgcc ccttctcgcg gtaggcgcga gcggctccct tcagcgccag
     2341 gttgttgctc tcggtggcgc ccgaagtgaa gacgatctcg cggggcgctc cgccgatcag
     2401 cctggccacc tggttccggc ccgtttcgac ggcctcggcc gcctgccatc cgtacacgtg
     2461 gctcgaggag gcggcattgc cgaactcgcc ggtgaggtac ggcatcatcg cctcgaggac
     2521 gacgggatcg atcggcgtgg tggcgttgtt gtcgaggtag atggggcggg tcatcgccca
     2581 gcagattagc cccggccccg gccgcggaca agtggcgccc gcggccgggg gtgtgcaccc
     2641 cgggttgtca gtgaagagag aa
//
LOCUS       DASVOD010000593         2612 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_41302, whole genome shotgun sequence.
ACCESSION   DASVOD010000593 DASVOD010000000
VERSION     DASVOD010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41302"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VF720_13075"
     CDS             <1..1299
                     /locus_tag="VF720_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX7880340.1"
                     /translation="ILITDERSGKKHEFKFDGGIVEFVKTLNHAKETLHPDAIYLHKT
                     RDEVDVEVAVQYNDTFNENVFAYVNNIHTVEGGTHLVGFKTALTRTINNYGTKNGLFK
                     DAKNGVSGDDVREGLTGVVSVKLRDPQFEGQTKTKLGNSEVQGIVASIVGEGLANFFE
                     ENPNVARRVLEKSVSAARAREAARKARDLARRKSLLDSGSLPGKLADCAERDPAKAEI
                     FLVEGDSAGGSAKMGRDRQYQAILPLKGKILNVEKARLDKMLSNEEIKVIITALGTSI
                     GTDEFNPDKCRYQRIILMSDADVDGSHIRTLLLTFFFRHMKPLIERGWVFVAQPPLYL
                     IKKGKDNHYAWSDAERDAILARIGEKGLHVQRYKGLGEMNPEQLWETTMNPLRRVVRR
                     VTMEDMVAAEEIFSTLMGEAVEPRRDFIYKNALNVQNLDV"
     gene            1371..>2612
                     /locus_tag="VF720_13080"
     CDS             1371..>2612
                     /locus_tag="VF720_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX7880341.1"
                     /translation="MADETGERIIAIPIEDEMQTSYLDYSMSVIVNRALPDVRDGLKP
                     VHRRILVAMNELNLAPDRPYRKSAKITGDVNGNYHPHGTTAIYDTMVRMAQDFSLRYP
                     LVDGQGNFGSVDGDAPAAERYTEGRLTRVAMEMLEDFEKETVDLRPNYDNTREEPIVL
                     PAKFPNLLVNGTAGIAVGMATNIPPHNLGEVVDAVIRVVDDPAIPVEDLLSIVTGPDF
                     PTGGIIMGRQGIRDCYTTGRGLIRVRARAEIEPWKNDRERIIVTEIPYMVNKSSLLED
                     IARLVREAAIPGISDLRDESDREGMRIVIEIKKDSPSQVVLNQLYKHTAMQQTFGAIN
                     LALVNNRPEILTLKQLIHHFIEHRKDVIVRRTKYDLAQAEKRAHILEGYKIALDNLDE
                     LIKLIRAARDAAEARVNMMARF"
BASE COUNT          555 a          880 c          815 g          362 t
ORIGIN      
        1 atcctcatca ccgacgagcg gagcggcaag aagcacgagt tcaagttcga cggcggcatc
       61 gtcgagttcg tgaagacgct gaaccacgcc aaggaaacgc tgcatcccga cgccatctac
      121 ctgcacaaga cgcgggacga agtggacgtg gaagtggccg tccagtacaa cgacaccttc
      181 aacgagaacg tgttcgccta cgtgaacaac atccacacgg tggaaggcgg cacccacctc
      241 gtcgggttca agacggcgct cacccggacg atcaacaact acggcaccaa gaacggcctc
      301 ttcaaggacg ccaagaacgg cgtctccggc gatgacgtcc gcgaaggcct gaccggggtc
      361 gtctcggtca agctgcgcga cccgcagttc gaagggcaga ccaagaccaa gctcggcaac
      421 tccgaggtcc aggggatcgt ggcctcgatc gtgggcgagg ggctggccaa cttcttcgag
      481 gaaaacccga acgtcgcccg ccgcgtgctc gagaagtcgg tcagtgcggc ccgggcccgc
      541 gaggcggccc gcaaggcgcg cgacctggcc cgccgcaaga gcctgctcga ttcgggcagc
      601 ctccccggca agctggccga ctgcgccgag cgcgatccgg ccaaggccga gatcttcctg
      661 gtcgagggcg actcggccgg cggttcggcc aagatgggac gcgaccggca gtaccaggcc
      721 atcctgcccc tcaagggaaa gatcctgaac gtcgagaagg cgcgcctgga caagatgctg
      781 agcaacgagg aaatcaaggt catcatcacg gcgctcggca cctccatcgg caccgacgaa
      841 ttcaacccgg acaagtgccg ctaccagcgc atcatcctga tgagcgacgc cgacgtggac
      901 ggcagccaca tccgcacgct cctcctcacc ttcttcttcc ggcacatgaa gccgctgatc
      961 gagcgcggct gggtgttcgt ggcccagccc cctctttacc tgatcaagaa ggggaaggac
     1021 aaccattacg cctggagcga cgccgagcgt gacgccatcc tggcgaggat cggcgagaag
     1081 gggcttcatg tccagcgcta caagggtctg ggggagatga accccgagca gctctgggaa
     1141 accaccatga accccctgcg gcgcgtcgtg cgccgggtca cgatggaaga catggtggcc
     1201 gccgaggaga tcttctcgac gctcatgggc gaggccgtcg agccgcgccg cgacttcatc
     1261 tacaagaacg cgctcaacgt acagaacctg gacgtctagg gtcgggcgcc cgccgagggc
     1321 cctcgcgaca cccgtcgtcc cgaactcata cgaggaaaga ggcttgagtc atggctgacg
     1381 agaccggcga acgaatcatc gcgataccca tcgaagacga gatgcagacg tcgtatctcg
     1441 actactcgat gtcggtcatc gtcaaccgcg ccctgccgga cgtccgcgac ggcctgaagc
     1501 cggtccaccg gcggatcctg gtggcgatga acgagctcaa cctcgccccg gaccgtccgt
     1561 accgcaagag cgccaagatc accggcgacg tgaacggcaa ctaccacccg cacggcacca
     1621 cggccatcta cgacacgatg gtccgcatgg cgcaggactt ctcgctgcgc tatccgctgg
     1681 tcgacggcca ggggaatttc ggctcggtgg acggggatgc cccggcggcg gagcggtaca
     1741 ccgaaggacg cctgacgcgc gtggccatgg agatgctcga ggacttcgag aaggaaaccg
     1801 tcgacctccg gccgaactac gacaacaccc gcgaggagcc gatcgtcctc ccggccaagt
     1861 tcccgaacct tctcgtgaac ggcacggccg ggatcgcggt cggcatggcc accaacatcc
     1921 ccccccacaa cctgggcgaa gtggtggatg ccgtcatccg cgtcgtcgac gacccggcga
     1981 taccggtgga ggaccttctt tccatcgtca ccggcccgga cttcccgacc ggcggcatca
     2041 tcatggggcg ccaggggatc cgcgactgct acacgacggg gcgcggcctg atccgcgtgc
     2101 gggcccgggc cgagatcgag ccgtggaaga acgaccgcga gcggatcatc gtcaccgaga
     2161 tcccctacat ggtgaacaag tcctcgctcc tcgaggacat cgcgcgcctg gtgcgcgagg
     2221 cggccatccc gggcatctcc gacctgaggg acgagtcgga ccgcgaagga atgcggatcg
     2281 tcatcgagat caagaaggac agcccgagcc aggtggtgct gaaccagctc tacaagcaca
     2341 cggccatgca gcagaccttc ggcgccatca acctggcgct ggtgaacaac cggccggaaa
     2401 tcctcacgct gaagcagctc atccaccact tcatcgagca ccgcaaggac gtcatcgtcc
     2461 ggcggacgaa atacgacctg gcccaggcgg aaaagcgggc ccacatcctc gagggataca
     2521 agatcgccct cgacaacctc gacgagctga tcaagctcat ccgcgccgcg cgtgacgcgg
     2581 ccgaggcacg cgtcaacatg atggcccggt tc
//
LOCUS       DASVOD010000594         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1033788, whole genome shotgun sequence.
ACCESSION   DASVOD010000594 DASVOD010000000
VERSION     DASVOD010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033788"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VF720_13085"
     CDS             complement(<1..1427)
                     /locus_tag="VF720_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX7880342.1"
                     /translation="MNTPRLGRREFLLKAGLAAGTMGVTTGAASLVGCGGGSSPGPVP
                     PGAPTDLVATAVSAARIDLAWADHATDETSYEIERRLATETIFTPLATIASDQVSYSD
                     EAVVERTMYVYRVRAVAEGLDSDYSNEADATTPATGAPLAPANVAVESVMADGAVITW
                     SDEAINESGYRLDRVDNVSVVPILTLPAGATRAELTGLSRDDLVRVLVVAFNELGESP
                     PSAEARFFTGAAAGDILFLVPEPNVLRVMWTDRSAGRVGFILERDLGSGFEVLATLPV
                     GTTTADDAGLSPGVTPTYRIRAADVFSIDSPTAAPDASMLRPARPTGLTTGLLDSNGS
                     RVVVEWQHPGPVDGFILERRVGTGAFTTISNTADPSLRRLEDNPQAVLVDVTYLVTAR
                     NAAGTSQSANEATIQPRAVFFFAASAALMTLKTVNKGVPVTVSRPSVSLGASCQASST
                     SIYLVRESESSIAAVVAHCTHECLRP"
     gene            complement(1504..>2006)
                     /locus_tag="VF720_13090"
     CDS             complement(1504..>2006)
                     /locus_tag="VF720_13090"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880343.1"
                     /translation="TFRTPRARSWGWETLAARRRESGDMAGAVQAYDAALAEQPENVR
                     LLRNMAGSLGRTGQSRRAADVLARLVTKVPDDAGAWRHLGMELAMVGSRDSAGAALRQ
                     ALELEPASVESLNELARILIAAPGTRREARALLARSLELAPNQPRSDDLRQMVERLDR
                     DGYGPG"
BASE COUNT          315 a          663 c          701 g          327 t
ORIGIN      
        1 ggccgcaggc actcgtgcgt gcagtgcgcc acgaccgccg cgatcgacga ctccgattcg
       61 cgcaccaggt agatgctggt ggaggaggcc tggcagctcg cgccaaggct cacggacgga
      121 cgactgaccg tgaccggcac ccccttgttc accgtcttga gcgtcatgag cgccgcgctc
      181 gcggcgaaga agaagacggc acgcggctgg atggtcgcct cgttggctga ttgcgacgtg
      241 ccggcggcat tccgcgcggt gacgaggtac gtaacatcca cgagcacggc ctggggattg
      301 tcctccaacc gccgcaacga cgggtcggcg gtgtttgaaa tcgtggtgaa agcgccggtg
      361 cctacccgac gttccaggat gaacccatct acgggtccag ggtgttgcca ctccactacg
      421 acgcggctgc cgttggagtc gagcagtccc gtggtcaggc cagtcggcct cgctggccgc
      481 aacatcgacg cgtccggcgc ggccgtgggc gaatcgatcg agaaaacgtc ggcagcgcga
      541 atgcgatatg tgggggtgac gcctgggctc aagccggcat cgtcagcggt cgtagtgccg
      601 actggcagtg tggcgagtac ttcgaatccc gatccgaggt cccgttcaag gatgaatccg
      661 acccgcccgg ccgagcgatc cgtccacatc acgcggagca cattcggttc cggcaccagg
      721 aacaggatgt ctcccgctgc cgctcctgtg aaaaaccgtg cctcagccga cggcggcgat
      781 tctcccagtt cgttgaaagc aacgacgagg acccgcacga ggtcgtcccg agaaagtccg
      841 gtcagttcgg cgcgcgtggc tcctgccggc agggtgagaa tgggcaccac ggaaacattg
      901 tcgacccgat cgaggcgata accgctttcg ttgatcgcct cgtcactcca ggtgatcacc
      961 gcgccgtcgg ccatcactga ctcgacggcc acgttggcgg gcgcgagcgg cgcaccggtc
     1021 gcgggcgtgg tggcgtccgc ttcgttggag tagtccgagt cgagcccttc ggccacagcc
     1081 cgcacccggt agacgtacat cgtccgttcg accaccgcct cgtcggaata gctcacctgg
     1141 tctgaggcaa tcgtggccag gggcgtgaag atcgtctcgg tggccagacg ccgctcgatc
     1201 tcgtaggacg tctcgtcggt ggcgtgatcg gcccaggcca ggtcgatgcg ggccgccgag
     1261 accgcggtgg ccacgaggtc cgtcggtgcg ccgggtggaa cgggtccggg cgaggagccg
     1321 ccgccgcaac cgacgaggga ggcggcgccg gtcgtcacac ccatcgtgcc cgcggcaagt
     1381 ccggccttga ggagaaattc gcggcgcccg agtcggggcg tgttcatcgg gatctccatt
     1441 cctcgctgac gagggggggc gcgcagcgga gggaaagtct atcaccggtt cctggtgcgt
     1501 gtgtcacccc ggcccgtagc catcccggtc gagccgctcg accatttgac ggaggtcgtc
     1561 gctcctcggt tggtttggcg ccagctcgag gcttctggcc aggagcgcgc gggcttcgcg
     1621 ccgggtgccg ggcgcggcga tcaggatgcg cgccagttca ttcaatgatt caaccgacgc
     1681 gggctccaat tccaaggcct gtcggagcgc ggccccggcg gagtcgcgcg atcccaccat
     1741 ggccagttcc atcccgaggt ggcgccacgc gccggcatcg tcgggcactt tggtcaccag
     1801 ccttgcgagc acgtcggcgg cgcggcgcga ctggccggtc cgtccgagcg agcccgccat
     1861 gttccgaagg aggcggacgt tttcgggctg ctcggccaac gcggcgtcgt aggcctgtac
     1921 ggcgcccgcc atgtcgccgg attcccgtcg gcgcgcggcc agcgtctccc aaccccagct
     1981 ccgggcgcgg ggggtgcgga acgtat
//
LOCUS       DASVOD010000595         9200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_300128, whole genome shotgun sequence.
ACCESSION   DASVOD010000595 DASVOD010000000
VERSION     DASVOD010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9200
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300128"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VF720_13095"
     CDS             <1..560
                     /locus_tag="VF720_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011238471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX7880344.1"
                     /translation="ANYIPAGMDITLHSENGLLGIGPFPFEGEEDPDLINAGKQTVTE
                     MPGASYFSSADSFAMIRGGHIDFSILGALEVDETGNLANWMIPGKMVKGMGGAMDLVA
                     GARRVVVTMNHAAKDGSPKILSRCSLPLTGVGVVHRIITELGVIDVTDKGLVLRERFD
                     NVAIDDIVKVTGAPLDVSNVAVISD"
     gene            596..1756
                     /locus_tag="VF720_13100"
     CDS             596..1756
                     /locus_tag="VF720_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017696497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX7880345.1"
                     /translation="MSYVELSEEHRLFQKTVRDFVDEVIKPRAKEIDEAGEFPWWCIE
                     ELRKMGLMGVPYGEEWGGAGGDTLMYAIAIEEISRASGSIGLLTAAHISLGTGPIYNF
                     GTDEQKKKYLPKLCSGQVLGAFCLTEPQAGSDAGGTRTVAVQDGDHWVINGAKNWITN
                     AGVADVFIVTAVTDKGQGTDGISSFIVEKGMPGLKTGKKEDKLGLRGSETNPVFFEDV
                     RVSNNAMLGPRGKGFKQFMKTLDGGRISIGAMAVGLAQAAMEASLEYAQTREQFNRPI
                     GHFQAIQFKLADMATEIAAARHLVYDSAVVKDAGGNFTRLSAMAKLFASEVAMRATTQ
                     AIQVYGGNGYSTEYPVERFFRDAKLCEIGEGTSEIQRMVIARELMKEYPVRR"
     gene            1785..2120
                     /locus_tag="VF720_13105"
     CDS             1785..2120
                     /locus_tag="VF720_13105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7880346.1"
                     /translation="MAVKLEWDGDIAILTPKGSLLGGDETVDLEKAIRDLSEKGNTKL
                     VIDLGQVDTMVSRPLGVLVAAHTNYVRRDGRIHLCNVENKLKNLMVITKLVAVFDMYN
                     TREGALGSF"
     gene            2467..3066
                     /locus_tag="VF720_13110"
     CDS             2467..3066
                     /locus_tag="VF720_13110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880347.1"
                     /translation="MKRLLVTVAALLALAAPAAHADKTKPTLYSAANVDGNIGEWDLT
                     NDFFANIYRAGKSDKPLEAKAYARYDCVEEVMYILYLTEPGVPLLVDGDAWIAIGAQN
                     NKVVNDDAGDDGVAPDFAFVGVNYDGDPNHARGYEASFPLAPGTYELIIHGNVFDDGL
                     KQTGATEGFPGDGIELDTTCTPVATESTTWGAVKSLIVQ"
     gene            3215..3496
                     /locus_tag="VF720_13115"
     CDS             3215..3496
                     /locus_tag="VF720_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7880348.1"
                     /translation="MAILKFRHKGLERFFLRGTTSGIQTKHAGRLRLILGRLNAAVEP
                     RDLALPGFELHPSRGQRKGTWAVSVSGNWRITFKFSGSDVEDVDYEDYH"
     gene            3504..3791
                     /locus_tag="VF720_13120"
     CDS             3504..3791
                     /locus_tag="VF720_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HEX7880349.1"
                     /translation="MSMHNPPHPGEILRELCLAPLGLNVTQAAAALGVSRKTLSSILN
                     GRAGISPEMAVRLSMAFDTTAESWLRQQIQYDLAQVEANRGSFRVKRLAAA"
     gene            3817..5424
                     /locus_tag="VF720_13125"
     CDS             3817..5424
                     /locus_tag="VF720_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX7880350.1"
                     /translation="MQPLSSQIRPSDPDFQANAAHHKGLSDALRERLERVRRGGPVDH
                     RKKHETRGKLFVRDRIERLLDPGTSFLEFSPLAAWDVYEDEVPAAGIVTGIGVVEGVE
                     CVIVANDATVKGGTYHPLTVKKHVRAQEIAFENRLPCVYLVDSGGAFLPLQADVFPDE
                     KHFGRIFYMQAHLSAAGIPQVAAVMGSCTAGGAYVPAMSDETVIVRGHGTIFLAGPPL
                     VKAATGEEVSAEDLGGAEVHSRSSGVTDHMAMDDAGALETVRSIVANLNRVKRLDIDV
                     RPPEPPAYDPEEIYGILPRDTRRPFDVKEIIARLVDGSRFHEFKALYGPTLVTGFARL
                     HGYPVGILANNGVLFSESAVKGAHFVELCSQRRIPLVFLQNITGFMVGRRYEAGGIAK
                     DGAKLVQAVATTAVPKFTVIIGGSFGAGNYGMCGRAYSPRMLFMWPNARISVMGGEQA
                     ASVLVTVKRDQLAAQGKTLSDAEAAEISRPILEKYEAEGNPYYSSARLWDDGVIDPAE
                     TRNVLALGLSAALNAPIPETKFPVFRM"
     gene            5435..6235
                     /locus_tag="VF720_13130"
     CDS             5435..6235
                     /locus_tag="VF720_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018082901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7880351.1"
                     /translation="MSWQTLEIESTGFVRRVWLNRPDVHNALNVEMIDELTRAFTGLG
                     ADDGARVIVLGGRGKSFCGGADISMMKAAGQMSPSENRAEARKLSAMFRAINECPKPV
                     IGRIHGVALGGGAGLTACVDVALTESACKFGFTEAKLGIMPAVISPFTVAKIGFSAAR
                     AHFTTGGRFPAVEALRIGLVHHVAVDAGELDDVVTRWIGEYRSAAPGSVTAIKDLLQR
                     LARPGGLDSPEVAEMTAELIAGRRASDEGKEGLASFLERRKPAWTEGE"
     gene            6232..7809
                     /locus_tag="VF720_13135"
     CDS             6232..7809
                     /locus_tag="VF720_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX7880352.1"
                     /translation="MSREFRRLLIANRGEIAVRLIRAARELGLETIAVHSDVDRAALH
                     VLEADRAVGIGAAPAVESYLNTSAILEAARKSGAEAIHPGYGFLSEKAEFAEAVINAG
                     LVWVGPPPEAMRLLGDKLGAKALAEKLGVPTVPGDDSERDRTDRSAEPEDQATADKAL
                     MARAMKVGFPLLLKAVAGGGGKGMRRVNDESELGEAIKAARREGFAAFGDSRLLVERF
                     VSPAHHVEIQVFADAHGQAIHLGERECSVQRRHQKIVEESPSPTITPKTRKAMADAAV
                     SLVKAAGYRSAGTCEFLLDESGNFYFLEVNTRLQVEHPVTEMVTGVDLARMQLVEATG
                     KPLPPTSATIEWRGHAIECRLYAEDPERGFLPSSGPVLMFKAPEGPGVRVDSGIMAGW
                     NVGVHYDPILAKIIVHDTSRDRAVVRMRRALAETVLLGLTSNLSFLQAVIGHPEFAAG
                     RTPIDFLDRHFAGWRAHDAGPSADVLALAAMAEWDRLGMIGGRLAASTNMNGATNGDV
                     NSPWHQLGPFRLGQKGR"
     gene            7812..8333
                     /locus_tag="VF720_13140"
     CDS             7812..8333
                     /locus_tag="VF720_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7880353.1"
                     /translation="MPKLDRKWIAGDVTRHVQAELSGDHLYMEIDGKPVEFSVVTGPT
                     GSFVLKDGDRILTGYAAATPDAIWVQLDGKMFVLDPEKPARARAGPAGPTGGDVISPM
                     TGTVRKVFAESGQSVQSGTPLLVVEAMKMEFTVTAPGEGRVAEVLCVAGQSVDLGQIL
                     VRFEAAPEPLPSE"
     gene            8642..>9200
                     /locus_tag="VF720_13145"
     CDS             8642..>9200
                     /locus_tag="VF720_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880354.1"
                     /translation="MDSVTTKNHCSRAELTCCSDAMTQGVLDTPYFLYVLVAPSDSMW
                     ARDTGIAGVQFGLNYQGAHDPSGNPSLTIDVFGWTSCATLEFRTPTPSWPNPGSGIII
                     TWDTTSVCQRKQVAVAGYFYMTAYQPGYFQLVPRPVDQQAKVVTCDAVERYVEMYNPP
                     TAAFSNGATYSGWNPCSGPCPTTGVQ"
BASE COUNT         1677 a         3032 c         3015 g         1476 t
ORIGIN      
        1 tggccaacta cattcccgcc gggatggaca tcacgctgca ctccgaaaac ggcctgctcg
       61 ggatcgggcc gtttcccttc gaaggcgaag aagatccgga cctcatcaac gccggcaagc
      121 agaccgtgac cgagatgccg ggcgcctcct acttttccag cgccgattcg ttcgccatga
      181 tccgcggcgg gcacatcgac ttttccatcc tcggcgctct cgaggtggac gaaacgggga
      241 acctggccaa ctggatgatc cccggcaaga tggtgaaggg gatggggggc gccatggacc
      301 tggtggccgg cgcccggcgc gtggtggtca cgatgaacca tgcggccaag gacggatcgc
      361 ccaagatcct gtcgcgctgc tcgctgccgc tcaccggcgt gggcgtggtg caccgcatca
      421 tcaccgagct cggcgtcatc gacgtgaccg acaaggggct cgtactccgt gaacgattcg
      481 acaacgttgc gatcgacgac atcgtcaagg tcaccggggc gccgctcgac gtctcgaacg
      541 tggccgtcat cagcgactga tccatccgca ccgggcaggg gacacgcatc gaaccatgag
      601 ctacgttgag ttgagcgaag agcaccgcct cttccagaag acggtccgcg acttcgtcga
      661 cgaagtcatc aagccccgcg cgaaggaaat cgacgaggcg ggcgagtttc cctggtggtg
      721 catcgaggag ctccggaaga tggggctcat gggagtgccc tacggcgagg agtggggagg
      781 cgccggcggc gacacgctca tgtatgccat cgccatcgag gagatcagcc gggcctccgg
      841 ttcgatcggg ctcctcacgg ccgcgcacat cagcctgggc accgggccga tctacaactt
      901 cggcaccgac gaacagaaga agaagtacct gccgaagctc tgctcgggac aggtgctcgg
      961 cgccttctgc ctcaccgagc cgcaggccgg ttccgacgcc ggcggcacgc gcaccgtggc
     1021 cgtgcaggac ggcgaccact gggtgatcaa cggcgccaag aactggatca ccaacgccgg
     1081 cgtggccgac gtcttcatcg tgaccgccgt caccgacaag ggacagggaa ccgacggcat
     1141 cagctcgttc atcgtcgaaa agggaatgcc ggggctcaag acgggcaaga aggaagacaa
     1201 gctcggcctg cgcggctccg aaaccaaccc ggtcttcttc gaggacgtgc gcgtgtcgaa
     1261 caacgccatg ctcggaccgc gcggcaaggg gttcaagcag ttcatgaaga cgctggacgg
     1321 cgggcgcatc tcgatcgggg ccatggcggt gggcctcgcc caggccgcca tggaggcaag
     1381 cctcgagtac gcccagaccc gcgagcagtt caatcggccg atcggccact tccaggccat
     1441 ccagttcaag ctggccgaca tggccaccga gatcgcggcg gcccgccacc ttgtgtacga
     1501 ctcggccgtc gtgaaggatg ccggcggcaa cttcacccga ctgtcggcca tggccaagct
     1561 cttcgcctcc gaagtggcca tgcgcgccac cacgcaggcc atccaggtgt acggcgggaa
     1621 cggctactcc accgagtatc cggtcgaacg cttcttccgc gatgccaagc tgtgcgagat
     1681 cggcgagggg accagcgaga tccagcgcat ggtcattgcc cgcgaactga tgaaggaata
     1741 tcccgtccgc cgctgagcgc ggcatcaccg aaacgaggta tcgaatggcc gtgaaactcg
     1801 aatgggatgg cgacatcgcc atcctcacgc ccaaggggag cctgctcggc ggcgatgaaa
     1861 cggtggatct cgagaaggcc atccgcgacc tgtccgaaaa ggggaatacc aagctggtga
     1921 tcgacctggg ccaggtcgac acgatggtgt cgcgcccgct cggcgtgctg gtggcggcgc
     1981 acaccaacta cgtgcgccgg gacggccgca ttcatctctg caacgtcgag aacaagctca
     2041 agaacctgat ggtcatcacc aagctggtgg cggtgttcga catgtacaac acccgggaag
     2101 gggcgctcgg gagcttctag gtccggggcg gctgtcgtgt cctggtattc ggggatttcc
     2161 cgattgtcat tcaagggata acagggggaa atgacccgag aattgcgccc gtttcaattg
     2221 attccgacaa tcatcccaac actttcagcc caggtgacgg tccgcttgac agttcaagcg
     2281 ccaaacgacg tccctgcaag caattaactt ctgcaatccg gacaaaaagt ggatgcgtac
     2341 cacgtacgac gtgggacaat tgatgcgtac ccgattccac acagcgatgg cagatctgcc
     2401 gagactcggc cgcggggccg gcaaccaaac tgccatcatc gtcacgacaa catgaggagt
     2461 aaggacatga agagactgct tgtgactgtt gctgccctgc ttgccctcgc cgccccggcc
     2521 gcccatgctg acaagaccaa gcccaccctg tattccgccg ccaacgtcga cggcaatatc
     2581 ggcgagtggg acctgaccaa cgacttcttc gccaacatct accgcgccgg caagtccgac
     2641 aagcccctcg aggccaaggc ctacgcccgc tacgactgcg tcgaggaagt catgtacatc
     2701 ctctacctga ccgagcccgg cgtgcccctg ctcgtggacg gcgacgcctg gatcgccatc
     2761 ggcgcccaga acaacaaggt cgtgaacgac gatgcgggcg atgacggcgt ggccccggac
     2821 ttcgcgttcg tcggcgtgaa ctacgacggc gatccgaacc atgcccgcgg ctacgaagcg
     2881 tcgttcccgc tggcgcccgg cacctatgag ctgatcatcc acggcaacgt cttcgacgac
     2941 ggcctcaagc agaccggcgc caccgaaggc tttcccggtg acggcatcga gctcgacacg
     3001 acctgcaccc cggtggccac ggaatcgacc acgtggggcg cggtgaagtc tctcatcgtc
     3061 cagtaagtca tccacggggc attcgcctcg cggatgaccc gtcgatggaa gaaacagaaa
     3121 ggcctcccgg cgcgatgccg gggggccttc tgtcgttggg gacgctgtcc cggtgtcgtt
     3181 gtaacctgat acgttacgtg ttacagttta tttcatggcg attctgaagt tcaggcacaa
     3241 ggggcttgaa cggttcttcc tgcgaggaac gacaagcgga atccagacaa aacatgcggg
     3301 ccgcctccgg ttgatacttg ggaggctgaa tgcggcggtc gaacctcgtg acctggccct
     3361 gcccgggttt gaattgcacc cctcgagagg acaacgcaag ggaacctggg cggtcagcgt
     3421 cagcggcaac tggcgaatca cgttcaagtt ctcaggctcg gacgtagagg acgtggacta
     3481 tgaagactac cactagtacc ggaatgagca tgcacaaccc accgcacccc ggggaaattc
     3541 tccgcgagct ttgcctggcc cctctcgggt tgaacgtgac gcaggccgcc gcggcgcttg
     3601 gcgtcagccg gaagacgctg tccagcatcc tgaatggccg ggccggaatc agtcccgaaa
     3661 tggcggtgcg gctttcgatg gcattcgaca cgactgctga aagctggttg aggcagcaga
     3721 tccagtacga tcttgcgcag gttgaagcca accgcggctc gtttcgtgtg aagcggcttg
     3781 cggcagcctg atgacccggg aatgagatag caacggatgc agccactttc cagccagatc
     3841 cgcccgtccg accccgattt ccaggccaac gccgcccatc acaagggcct ttcggacgcg
     3901 ctccgggagc gcctggagcg cgtgcggcgc ggcggtccgg tcgatcaccg gaagaagcac
     3961 gagacgcgcg gcaagctctt cgtgcgcgac cggatcgagc ggctgctcga cccggggacg
     4021 tcgttcctcg aattttcccc cctggccgcc tgggacgtgt acgaagacga agtgccggcc
     4081 gccgggatcg tgaccggcat cggggtggtg gagggggtgg aatgcgtcat cgtggccaac
     4141 gacgccacgg tgaagggggg cacgtatcac ccgctgacgg tcaagaagca cgtgcgggcg
     4201 caggagatcg ccttcgagaa ccggttgccc tgcgtctacc tggtcgattc gggcggggcc
     4261 ttcctgccgc tccaggccga cgtgttcccg gacgagaagc acttcggacg gatcttctac
     4321 atgcaggccc acctgtcggc tgccgggatt ccgcaggtgg cggcggtgat gggttcctgc
     4381 acggccggcg gggcctatgt gccggccatg agcgacgaga cggtcatcgt ccggggccac
     4441 ggcacgatct tcctggcggg gccgcccctg gtgaaggccg ccaccggcga ggaggtcagc
     4501 gccgaggacc tcgggggcgc cgaggtgcac agccgctcga gcggcgtcac cgaccacatg
     4561 gcgatggacg acgcgggcgc gctggaaacg gtgcgctcca tcgtggccaa cctgaaccgg
     4621 gtgaagcggc tcgacatcga cgtccgcccg ccggagcccc cggcctacga tcccgaggag
     4681 atctacggga tcctgccgcg cgatacgcgc cgcccgttcg acgtgaagga gatcatcgcc
     4741 cggctcgtcg acggcagccg cttccacgag ttcaaggctc tgtacggccc gacgctcgtc
     4801 accggctttg cgcggctgca cggctacccg gtgggcatcc tggccaacaa cggcgtgctc
     4861 ttttcggaga gcgccgtgaa gggcgcccac ttcgtcgaac tgtgcagcca gcgcaggatc
     4921 ccgctggtct tcctgcagaa catcaccggc ttcatggtgg ggcggcgcta cgaggcaggc
     4981 ggcatcgcca aggacggcgc caagctggtg caggcggtgg ccacgacagc ggtgcccaaa
     5041 ttcaccgtca tcatcggcgg ctcgttcggc gccggcaact acggcatgtg cggacgcgcc
     5101 tattcgccgc gcatgctctt catgtggccc aacgcgcgca tctcggtgat gggaggggag
     5161 caggcggcca gcgtgctcgt caccgtcaag cgcgaccagc tcgcggcgca ggggaagacg
     5221 ctctccgacg ccgaggccgc cgaaatctcc cggccgatac tcgagaagta cgaggcggag
     5281 ggaaacccct actactcgtc tgcccgcctg tgggacgacg gcgtgatcga cccggcggag
     5341 acacgcaatg ttctggcgct gggattgtcc gcggcgctca acgcaccgat cccggagacg
     5401 aagttccccg tcttccggat gtgaggcaac caccatgagc tggcagactc tcgagatcga
     5461 atcgaccggc ttcgtccgcc gcgtatggct gaaccgcccg gatgtacaca acgcgctcaa
     5521 tgtcgagatg atcgacgagc tgacgcgcgc gttcaccggg ctcggcgccg acgacggcgc
     5581 ccgcgtcatc gtgctcgggg gtcgcggcaa gtcgttctgc ggcggcgccg acatcagcat
     5641 gatgaaggcc gccgggcaga tgtccccgtc ggagaaccgg gccgaggcgc gcaagctctc
     5701 ggccatgttc cgggccatca acgaatgccc gaagccggtc atcggacgga tccacggcgt
     5761 ggcgctcggc ggcggcgcgg gactcaccgc gtgcgtggat gtagccctga cggagagcgc
     5821 ctgcaagttc ggcttcaccg aggccaagct cggcatcatg cccgcggtca tttccccgtt
     5881 cacggtggcc aagatcggct tctcggccgc gcgcgcccat ttcaccaccg gcgggcggtt
     5941 cccggccgtc gaagccctgc gcatcggact cgtccaccac gttgcggtcg atgccggcga
     6001 actggatgac gtggtgaccc gctggatcgg cgagtaccgg agcgcggcgc ccggttcggt
     6061 gacggccatc aaggacctgc tccaacggct ggcgcgcccc ggtggcctcg acagccccga
     6121 agtggcggag atgacggccg agctgatcgc cggtcgccgc gcttccgacg aggggaagga
     6181 agggctggcc tcgttcctcg agcgcaggaa gcccgcctgg accgagggcg aatgagccgc
     6241 gagttccgac ggctcctcat cgccaaccgc ggggagatcg ccgtccggct gatccgcgcc
     6301 gcgcgcgagc tcgggctcga gacgatcgcg gtgcactccg acgtggaccg cgccgcgctc
     6361 cacgtgctcg aagccgatcg cgcggtgggg atcggggcgg cgccggccgt cgagtcctac
     6421 ctcaatacca gtgccattct cgaagccgcc aggaagtcgg gcgccgaggc catccatccc
     6481 ggctacggat tcctgtcgga gaaggccgag ttcgccgagg cggtcatcaa tgcgggcctc
     6541 gtgtgggtgg ggccgccccc cgaggcgatg cgcctgctgg gcgacaagct cggcgccaag
     6601 gcgctggcgg aaaagctcgg cgtgccgacg gtgccggggg atgattcgga acgcgatcgt
     6661 acggatcgga gcgcggagcc agaagatcag gcaactgccg acaaggcgct catggcccgg
     6721 gcgatgaagg tcggctttcc gctcctcctc aaggcggtgg cgggaggcgg cggcaaggga
     6781 atgcggcgcg tgaatgacga gtccgagctg ggcgaggcca tcaaggcggc ccgacgcgag
     6841 ggttttgctg ccttcggcga cagtcgcctg ctcgtcgaac gattcgtgag cccggcgcac
     6901 cacgtggaga tccaggtctt cgccgatgcg catggccagg ccatccatct cggcgagcgc
     6961 gaatgcagcg tgcagcggcg gcaccagaag atcgtggaag agagcccctc tcccaccatc
     7021 acgccgaaaa cgcgcaaggc catggccgat gccgcggtga gcctcgtcaa ggcggccggc
     7081 taccggagtg cgggcacctg cgaattcctg ctcgacgaat cgggcaattt ctatttcctc
     7141 gaggtgaaca cgcggttgca ggtggagcat cccgtcaccg aaatggtcac cggcgttgac
     7201 ctggcgcgca tgcagctggt ggaagccacc ggcaagccgc tgccgccgac ctcagctacc
     7261 atcgagtggc gcggtcacgc catcgagtgc cggctgtacg ccgaggatcc cgagcgcggc
     7321 ttcctgccgt cgagcggccc ggtcctcatg ttcaaggcgc ccgaggggcc cggcgttcgt
     7381 gtcgattccg gcatcatggc cggctggaac gtgggcgttc actacgaccc gatcctggcc
     7441 aagatcatcg tgcacgacac ctcgcgcgat cgggcagtcg tccgcatgcg gcgcgcgctg
     7501 gcggagacgg tgctgctcgg gctcacgagc aacctgtcgt ttctccaggc ggtcatcggt
     7561 catcccgagt tcgcggcggg ccgcacgccc atcgacttcc tcgatcgcca cttcgccggc
     7621 tggagggccc acgacgccgg cccttcggcc gacgtcctcg cgctcgcggc catggcggag
     7681 tgggaccggc tcggcatgat cggcgggcgc cttgccgcct cgaccaacat gaacggtgcg
     7741 acgaacggcg acgtgaactc cccgtggcac cagctcggcc ccttccgtct cggccagaag
     7801 gggcgctgac catgccgaaa ctcgatcgca agtggattgc cggtgatgtc acgcgccacg
     7861 tgcaggccga actctcgggc gatcatctct acatggagat tgacggcaag ccggtggaat
     7921 tcagcgtcgt gaccggcccc accggctcgt tcgtcctcaa ggacggagac cgcatcctga
     7981 ccggttacgc cgccgccacc cccgacgcca tctgggtgca gctcgacggc aagatgttcg
     8041 tcctcgaccc cgagaaaccc gcccgcgcca gggccggtcc cgccggcccc accggcggcg
     8101 acgtcatcag cccgatgacc ggaaccgtcc gcaaggtatt cgcggaatcg ggacagtcgg
     8161 tgcagtcagg cacgcccctg ctcgtcgtcg aggcgatgaa gatggagttc accgtcaccg
     8221 ctcccggcga gggacgggtg gccgaggtgc tctgcgtggc ggggcagtcg gtggacctgg
     8281 ggcagatcct ggtgcgattc gaggcagcgc cggagcccct tccatcggaa taacgcccgg
     8341 aagtaacgtt ccggaatcgg gcagcctccg cccggcatgc ctatattagt tctggacgtc
     8401 cgctccccat ggaatccccc gggaggactc gatcatgact gccgcgtcgc tgcgcccctg
     8461 cccgctgccc accgcattgg gcctgaaggg tcgagccgac gacgcggggc cggatcaaga
     8521 cgaccttggt cggccaatta cggggcccca ctggttgttg atcctgttcg tagcatgcag
     8581 ccttgggatc gcgccgcaag catgggcggg tcccggtcac tctcccagaa ttcttctcca
     8641 cgtggattcg gtaaccacga agaaccactg cagccgggcc gagctgacct gctgttcgga
     8701 tgcgatgacg cagggggtgc tcgacacgcc atactttctc tacgtactcg tcgcgccgtc
     8761 cgattccatg tgggccaggg ataccggcat cgccggagtg cagttcggac tgaactacca
     8821 gggtgcccac gacccttccg gcaatccctc cctcaccatt gatgtctttg gctggacttc
     8881 ctgcgcgacc ctggaattcc ggacgccaac tccgtcgtgg cccaatcccg ggagcggcat
     8941 cattatcacc tgggacacga cgagcgtctg ccagcgcaaa caggtcgccg tggcgggcta
     9001 cttctacatg actgcctacc agccaggcta cttccaactg gttcctcgcc cggtggatca
     9061 gcaggccaag gtcgtgacgt gcgacgccgt cgagcgttac gtcgagatgt acaacccacc
     9121 aaccgccgcg ttcagcaacg gagccaccta ttccgggtgg aatccctgca gcggcccctg
     9181 cccgacaacc ggcgtccagt
//
LOCUS       DASVOD010000596         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_300143, whole genome shotgun sequence.
ACCESSION   DASVOD010000596 DASVOD010000000
VERSION     DASVOD010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300143"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..2021
                     /locus_tag="VF720_13150"
     CDS             57..2021
                     /locus_tag="VF720_13150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7880355.1"
                     /translation="MTPDVPDPDATRAFPTPSGPTSGPDKSSGPELPVVHAKTGEDLP
                     ETAASQLKAGQVLFNRYALKRVLGRGGFGVVWMADDRELEMEVALKFLADHIASSPDS
                     VQDLKGEARRSLRLTHPNIVRIHNFIQEGTLVGISMEYVPGGSLPQIRAARGLRTFSL
                     EEMRPLIGQLCDALEYAHTKPRIVHRDLKPANLMLDARGELKIADFGISRSISDSHTR
                     LTSPGDTSGTLAYMSPQQMLGESPKPADDIYSLGATIFDLLTGKPPFHTGDIVRQALD
                     MLPPRMTIRRREMGIEGEDIPEDWEQVVAACLAKDAADRPTSALAVAEHLGIRLPSGS
                     SGSVRSATSASAAALAGETVVLGNSAPASARNSSTGPTAPVRGTTPTSPARGPGTTAN
                     EGAVAPGYPAARPTVGMPKWPLYAGGAALVLVVLALAVFRPWESGSAPVVTQRVEPPP
                     PIEIQAPPPAPVADPNAGTATTPGTSDLPVIPTGRPPVDPPPESKPAKPKDPEPSRPA
                     ETRPEPPPARPEPTPAATETKPSPTPVKPPAVSGAETSSAEPAIRSLLHQYEQALEAL
                     DLGAYASLWVSLPEKSRSTLANSFRDMSSMSIDIANVQIEATGATAVARFKESRTITP
                     KAGPKQSVNRDVRLELTSAGGGWKISRSGS"
     gene            complement(2024..>3404)
                     /locus_tag="VF720_13155"
     CDS             complement(2024..>3404)
                     /locus_tag="VF720_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX7880356.1"
                     /translation="TITNTGGSTLTGTLAESCDDYVILAGGGAFSLGAGQSRLATIRF
                     RPMSAGDKPCTLSVGTGCPGVPLSGTGQAPPACQLSQPSLTFPKTILGESSERTFMIT
                     NAGGGTLAGTVTSPCNEFAVIGGGAYSLTAGQSTTVTVRFTPAQGGSRFCTLETGTTL
                     CADLPTSGVGDPPPVCEVSPASLAFGTVGIGASADRSFTITNDGGQTLTGSVSLSGCP
                     EFSIVSGAGNYVLIGGQQHTVTVRLTPASGGPKSCAIETGSATCADVSAAGTGEALPV
                     CALSTTSLSFGLVNVGATSDMSFMISNTGGGTLTGNVTESCADFSLIAGGGNFSLNMG
                     QSRTVTVRFAPGSFDAKSCTIDLGTNCADVSASGTGAVSYASHVRPIIVNSGCSGGSC
                     HGGTQIDLTVFANAVMKTNAGDPPNSLILIKPGPPPGNHAGGEFANFATNQPDYQTIL
                     KWIIQGVRP"
BASE COUNT          551 a         1166 c         1179 g          508 t
ORIGIN      
        1 gtgtatgatt cgaccctcat gttcgcatcg tcgcccaatg gcccacggtc accccgatga
       61 cccccgacgt cccggatcct gacgccacgc gggcgtttcc cacgcccagc gggcccacgt
      121 ccgggccgga caagtcctcc ggccccgagc tccccgtcgt ccacgcaaag accggggagg
      181 atcttcccga aaccgccgcc agccagctca aggccggcca ggtcctcttc aatcgatacg
      241 cgttgaaacg cgtgctgggg cggggcggat tcggcgtggt gtggatggcc gatgatcgcg
      301 agctggaaat ggaagtggcg ctgaagttcc tggccgatca cattgcgtcg agcccggact
      361 cggtgcagga cctgaagggt gaggcgcggc ggagcctccg gctcacgcat ccgaacatcg
      421 tccgcattca caacttcatc caggaaggga cgctggtcgg aatcagcatg gagtacgtgc
      481 cggggggctc cctgccgcag atccgggcgg cgcgggggct tcgcacgttt tccctcgagg
      541 agatgcgccc cctgatcggc cagctctgcg atgcgctcga gtacgcgcac accaagcccc
      601 ggatcgtcca tcgcgatctc aagcccgcca acctgatgct cgacgcgcgg ggcgagctca
      661 agatcgccga cttcggcatc tcgcggagca tttcggattc gcacacccgc ctgacgagcc
      721 cgggcgatac gtcgggaacg ctggcgtaca tgagcccgca gcagatgctg ggagaatcgc
      781 cgaagccggc ggatgacatc tactcgctgg gcgccaccat cttcgatctc ctgaccggca
      841 agcccccgtt ccatacgggc gatattgtgc gacaggcgct cgacatgctg ccgccccgca
      901 tgacgatccg ccgccgggag atggggatcg agggcgaaga cattcccgag gattgggaac
      961 aggtggtggc cgcgtgcctg gccaaggatg cggccgatcg tcccaccagc gccctggcgg
     1021 tggccgagca cttgggaatc cggctcccct cgggctcgtc ggggtcggtg cggagcgcca
     1081 cgagtgcttc ggctgcggca ctggcgggcg agaccgtcgt tctcgggaat tcggccccgg
     1141 cctcggcacg caactcctcc accggaccga cggcgccggt ccgaggcacg acaccgacat
     1201 cgccggcgcg tggccccggc acgacagcga acgagggcgc tgtcgcgccc gggtacccgg
     1261 ccgcgcgccc gacggtcggg atgcccaagt ggcctcttta cgccggcggc gcggcgctcg
     1321 tgctggtcgt cctcgcgctg gcggtgttcc ggccgtggga gtcggggtcg gcaccggtcg
     1381 tcacgcagcg ggttgagccg ccgcctccga tcgagataca ggcgccgccg cccgctccgg
     1441 tcgccgatcc gaatgccggc acggccacga cgccgggcac gagtgacctc cccgtcatcc
     1501 cgacaggtag accaccagtc gatccgccgc ccgagtcgaa accggccaag ccgaaggacc
     1561 cggagccatc gcgcccggcc gaaacgcgcc ccgagccgcc acccgcaaga cccgagccga
     1621 cgccggccgc caccgaaacg aagccgtctc ccacgccggt caagccaccc gccgtgagcg
     1681 gcgccgaaac atcgagtgcc gaaccggcca tccgcagttt gctgcaccag tacgagcagg
     1741 cactcgaggc gttggacctc ggggcttacg cctcgctctg ggtgagcctt cccgagaagt
     1801 cccggagcac gctcgcgaat tcgttccgcg acatgtcctc gatgtcgatc gacatcgcca
     1861 acgtgcagat cgaggccacg ggcgcgaccg cggtggcgcg cttcaaggag agccgcacga
     1921 tcacccccaa ggcggggccg aagcagtcgg tcaaccggga cgtgcggctg gagcttacga
     1981 gtgccggcgg cggctggaag atcagccggt cgggatcgta gccctacgga cgcactccct
     2041 ggatgatcca cttcaagatc gtctggtagt ccggctggtt cgtggcgaag ttggcgaact
     2101 cgccgccggc gtggttgccg ggtggaggcc cgggcttgat caggatcagg ctgttcggcg
     2161 ggtcgcccgc gttcgtcttc atcaccgcgt tggcgaacac cgtaaggtcg atctgcgtgc
     2221 cgccgtgaca gctcccgccc gagcaacccg agttgacgat gatcgggcgg acatggctgg
     2281 catagctcac ggcgccggtt cccgaggcgg agacgtcggc gcaattggtg ccgaggtcga
     2341 tggtgcagct cttcgcgtcg aagctgccgg gcgcgaagcg gacggtgacc gtgcggctct
     2401 gtcccatgtt gaggctgaag ttgccgccgc cggcgatcag cgagaaatcg gcgcagctct
     2461 ccgtaacgtt gccggtgagc gtcccgccgc ccgtgttgga gatcatgaag ctcatgtccg
     2521 acgtggcgcc cacgttcacc aggccgaacg acaggctggt ggtcgagagg gcgcacacgg
     2581 ggagcgcttc gccggtgccg gcggccgaca cgtccgcgca ggtggcgctg ccggtttcga
     2641 tggcgcagct cttcgggcca cccgaggccg gcgtgagcct cacggtcacg gtgtgctgct
     2701 gcccgccgat caggacatag ttgccggccc cggacacgat cgagaactcg gggcaaccgc
     2761 tcagggaaac gcttcccgtg agggtctgtc ccccatcgtt ggtgatcgtg aacgaccggt
     2821 ccgccgacgc gccgatgccg acggtgccga aggccagcga cgcaggtgac acctcgcaaa
     2881 cgggtggcgg atcccccacg ccggaagtcg gaagatcggc gcagagcgtc gtgccggttt
     2941 ccagcgtgca gaaacgactc cctccctggg ccggcgtgaa gcgaacggtg accgtcgtcg
     3001 actggccggc cgtcagcgaa taggcgccgc cgccgatgac cgcgaattcg ttgcacggcg
     3061 aggtgacggt tcccgccagg gtgccgccac ccgcgttggt gatcatgaac gtccgttccg
     3121 aggactcgcc gaggatcgtc ttggggaagg tgagcgaggg ctggctcaac tggcaggctg
     3181 gtggcgcctg gcccgtaccc gaaagcggca cgcccgggca ccccgtgccg accgagagcg
     3241 tgcagggctt gtcgccggcg gacatcggcc ggaagcggat ggtggccagg cggctctggc
     3301 ccgcgccgag gctgaacgcg ccgccgccgg ccaggatcac gtagtcatcg cacgactcgg
     3361 ccagcgtgcc ggtaagcgtg gagccgccgg tgttcgtgat cgtg
//
LOCUS       DASVOD010000597         2909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_300248, whole genome shotgun sequence.
ACCESSION   DASVOD010000597 DASVOD010000000
VERSION     DASVOD010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300248"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..481
                     /locus_tag="VF720_13160"
     CDS             11..481
                     /locus_tag="VF720_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880357.1"
                     /translation="MKRVVLLSAALLGVTALPARALPVLDFQLGAHYVEPTGDIPRGD
                     VEDAYGSGFGAYGRMRFPIGPARLAGAVTWNQLDGNGDVDDLDFLTFQVGPHYSFAVV
                     SVGAELVWLTEFDELGYSPMVSVTVLKFEGTLAYTAAFESDSAEYVTFGIGWRF"
     gene            complement(485..1618)
                     /locus_tag="VF720_13165"
     CDS             complement(485..1618)
                     /locus_tag="VF720_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880358.1"
                     /translation="MRLTLVHAMAAGAAALLMFTLLPAPVRAQSTSFTYQGLLDNGAG
                     TASGLHDFQFRLFALSAAGVDIAPPVCIDNVMVDNGVFTAQLDFGQAYITTAERWVEV
                     SVRQDTGLGCGDPTGFVTLAPRQPITAAPMASHARSAFALDAADGNPASAVYVDDAGK
                     VGIGTTTPAWSLHVKALGPVLVLQDTNTPANQAGYVTFWNNVPTETAWVGFGSPGSAD
                     FSMYNGRAGGDLLFYAGGSRVILARSNGDVGIGTGAAAPTGKLDVRGDIKLGPTGQYQ
                     ATCGEEKLRVVRGKVSAAGGILLGSGFTASRSSVGTYLISFSPAFPAGQAPAVTVSAE
                     WTSGSAYIAMTNGVLSSGTGVRVTNGSGTLVDQVFYFTAVGLR"
     gene            complement(1642..2217)
                     /locus_tag="VF720_13170"
     CDS             complement(1642..2217)
                     /locus_tag="VF720_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7880359.1"
                     /translation="MDVRSGSVRRLRSTLAVAWLIGSAGSAHAQFAISRFTIDGGGGT
                     RSTGGAFALGGTMGQPDAGRLTSLSFTLSGGFWFGGIAVSAVPPGDGIGPPAPPGPPF
                     RVLPATPNPVVERTVIAFDLPEPAGVRVSLFDVSGRLLRVLVDEPLTAGTHRTSWDRR
                     DGSGRRVVPGIYFLRLDAGAHQGNERIVVVP"
     gene            complement(2534..>2909)
                     /locus_tag="VF720_13175"
     CDS             complement(2534..>2909)
                     /locus_tag="VF720_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX7880360.1"
                     /translation="FSGLLGTTTAAHIHAPTAVPLAGTAGVATTTPSFVGFPLGVTSG
                     SYMATLNTLNSSSYNPSYVTANGGTAASAEAALFAAIRAGRAYFNIHSTSVGSGEIRA
                     FLIPTPTPVEPASWSSIKKLLQ"
BASE COUNT          483 a          931 c         1019 g          476 t
ORIGIN      
        1 ggaggagtga atgaaacgag tcgtgctgct cagtgcggcc ctgctgggcg tgaccgcgct
       61 tccggcccgg gcgctgccag tcctggattt ccagctcggg gctcactacg tcgagccgac
      121 cggggatatc ccgaggggcg acgtggaaga cgcgtacggc agcggtttcg gcgcctatgg
      181 ccggatgcgg tttccgatcg ggcccgcacg gttggcgggc gccgtcacct ggaatcaact
      241 ggatggcaat ggagacgtcg atgatctcga cttcctcacc ttccaggtcg gcccgcatta
      301 ctcgtttgcg gtcgtgtcgg tcggcgccga gctggtctgg ctgacggaat tcgacgagct
      361 cggctactcg ccgatggttt ccgttaccgt cctcaagttc gaggggacgc tggcctacac
      421 cgcggcattc gaatccgaca gcgccgaata cgtcacgttt ggcatcggat ggcggttctg
      481 atccctaccg aagccccacc gccgtgaagt agaaaacctg gtccaccagc gtgccgcttc
      541 cattggtgac gcggacaccc gttcccgacg acaggacccc gttggtcatg gcaatgtagg
      601 ccgagccgct cgtccactcg gccgacaccg tcaccgccgg ggcctggccg gcggggaagg
      661 cgggactgaa gctgatgagg taggtcccga cgctcgagcg cgaggccgtg aagccgctgc
      721 cgagaaggat gcctcccgcc gcggacacct ttccccgcac gacgcgcagt ttttcctcgc
      781 cgcaggtggc ctggtactgg cccgtcggcc ccagcttgat gtcgccgcgg acgtcgagct
      841 taccggtggg ggcggcggcc cccgtaccga tgccgacgtc gccgttgctt cgggccagga
      901 tgacgcggct gccgccggcg tagaagagaa gatcgccgcc ggcgcgcccg ttgtacatcg
      961 agaaatcggc gctccccggg ctcccgaagc ccacccaggc ggtttcggtc ggcacgttgt
     1021 tccagaaggt gacatagccg gcctggtttg ccggcgtgtt cgtgtcctgg aggaccagga
     1081 ccggcccgag ggccttcacg tgaagggacc aggcgggagt ggtggttccg atgcccactt
     1141 tcccggcatc gtccacatag accgcgctgg cggggtttcc gtccgccgca tcgagcgcga
     1201 aggccgacct ggcgtggctg gccatcgggg ctgcggtgat cggctgtcgc ggggcgaggg
     1261 tcacgaaccc ggtgggatcg ccgcaaccga ggccggtgtc ctgccggacg ctcacctcca
     1321 cccaccgttc ggcggtcgtg atatacgcct ggccgaagtc gagctgggcg gtgaagacgc
     1381 cgttgtccac catcacgttg tcgatgcaga cggggggcgc gatgtcgacg ccggccgcgg
     1441 agagggcgaa aagccggaac tggaaatcgt gcaggccgct ggccgtcccc gcgccgttgt
     1501 cgaggagccc ctggtaggtg aaggacgttg actgtgcccg gaccggggcc ggcagcagcg
     1561 tgaacatgag cagtgcggcc gcgccggccg ccatcgcgtg aacgagtgtc agcctcatcg
     1621 cgaactcctt tgtccggtgt ctcaaggaac caccacgatt ctctcgttgc cctggtgagc
     1681 gcccgcgtcc aacctcagga agtagatgcc cgggacgaca cgtcggcccg atccgtcacg
     1741 ccgatcccac gatgtccggt gcgttccagc ggtgagcggc tcgtcgacca ggactcgcag
     1801 gagtcgtccg gagacgtcga acagcgagac gcggacaccc gcgggctcgg gcaggtcgaa
     1861 ggcaatcacc gtccgttcga caaccgggtt gggagttgcc gggaggacac ggaacggagg
     1921 tcccggagga gcgggcggtc cgatcccgtc gccggggggc acggccgaaa cggcgatccc
     1981 gccgaaccag aagccgccgc tcaaggtgaa gctcaggctg gtcaggcgtc ccgcgtcggg
     2041 ctggcccatc gttccaccca gggcaaaagc gccgcccgtg gagcgtgttc cgccgccacc
     2101 atcgatggtg aatcgcgaaa tcgcgaattg tgcgtgagcg gatcccgccg agccgatgag
     2161 ccaggcgacc gccagggtcg agcggagcct gcgaaccgat ccggatcgaa cgtccatgtt
     2221 tcctcctggg ctcgagtcac cggttcatgc gagaaaacca tccggcaggt gacatgggaa
     2281 ggataaagtc caaaggcgca ttcgtgtcgt cctaacggcg gatcaccacc ttccgcgtgc
     2341 cggcgcccag cacgctttcg atccggacga ggtaaagccc ggccggcacg aggaggcctg
     2401 tgtcgtcacg cgtatcccag tcgagtcggt gctcgccggc tcgatccgtc cccgcgcttc
     2461 cgagcctccg acgaacagca caacggccac cccggttcga gggtggccgt tgaatgtcag
     2521 gtcgtcggtt cgactactgg agcagcttct tgatcgagct ccagctcgca ggctcgaccg
     2581 gtgtcggagt cgggatcagg aaggcgcgga tttcgccact gccaaccgac gtcgagtgga
     2641 tattgaagta ggccctgccg gcgcggatgg ccgcgaagag agccgcttcc gccgaggccg
     2701 cagtcccgcc gttggcagtg acgtaggagg ggttgtagct ggatgagttg agcgtgttga
     2761 gggtagccat gtacgaaccg ctcgtgactc ccagcgggaa gcccacgaac gacggtgtgg
     2821 tggtggccac acccgcggtg cccgcgaggg gcacggcggt cggcgcatgg atgtgagccg
     2881 cggtggtggt gccgaggagg ccactgaag
//
LOCUS       DASVOD010000598         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_144896, whole genome shotgun sequence.
ACCESSION   DASVOD010000598 DASVOD010000000
VERSION     DASVOD010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144896"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1738
                     /locus_tag="VF720_13180"
     CDS             <1..1738
                     /locus_tag="VF720_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEX7880361.1"
                     /translation="ESEVRAAFSDLTRYDLDRWLEHGRQVAAEREAARAEGRHPDLER
                     LVKAWGGVSIVYRKRLQDSPAYRLNHEEVENALTEGIGFAECLSPVEFLAGADGRVAS
                     VRFERQALVDGRWSATGDILELPARTVQVAAGTHPNAIYEREHPGTFVMDRWGEYFRP
                     HCRTADGVALEAVPEGGLGFFTSYDQGGRRITYYGDNHPVYVGNVVKAMASARDGSRE
                     VTALFAPELARLDPADQADRDRAFAELNDRLDDLLVARVEENRRLAPGVIELVLRAPM
                     AARRFRPGQIFRLQNYETRADQVDGHPLTMEGLPVTGAWADPVAGLVGVVVLETGGSA
                     RLAALLVPGEPVVLMGPSGAPSDIPENESVLLVGAGFGVAVIPSLAKAMRERGNHVTA
                     VAGFRSAVDLFYRDAIEGATDRVIWCTEEGAAIEPRRPGDRAATGTPIEGLQAWASGE
                     LRVDDPALAGTEHLLAVGPDALLSALGSARRNGLEAPFGTARTAVASINAPMQCMMKE
                     ICAQCLTRHVDPETGKESYVFSCFRQDQPLDRVDFAHLAARLSQNATSETLTHLWIDR
                     LLARRAAGSGAP"
     gene            1735..>2154
                     /gene="trxA"
                     /locus_tag="VF720_13185"
     CDS             1735..>2154
                     /gene="trxA"
                     /locus_tag="VF720_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX7880362.1"
                     /translation="MTATASFVLDVTERDFEAQVIEKSKSVPVVVDFWAPWCGPCRAL
                     GPVLEKLAAEWNGGFVLAKLNTDEAPYISQALRISSIPLVMAFHEGQVVDEFVGALPE
                     AQIREWLTRLAAPMAADATTAEATAANDTGAARADRLR"
BASE COUNT          327 a          787 c          729 g          311 t
ORIGIN      
        1 cgagtcggag gtccgggccg ccttcagcga cctgacccga tacgacctcg accggtggct
       61 cgagcacggt cgccaggtgg ccgccgagcg cgaggcggcc cgcgccgagg ggcgccaccc
      121 cgacctcgag cgcctggtca aggcctgggg cggcgtgtcg atcgtctacc gcaagcgctt
      181 gcaggactcg ccggcctatc gcctcaacca cgaggaagtg gagaacgcgc tcaccgaggg
      241 gatcggcttc gccgagtgcc tctcgccggt ggaattcctc gcaggagccg atggccgcgt
      301 ggcctcggtg cgcttcgaac ggcaggcgct ggtcgacggt cgctggtcgg ccaccggcga
      361 catcctcgag ctccccgcgc gcacggtgca ggtggcggcg ggcacgcatc ccaatgccat
      421 ctacgagcgg gagcatcccg gaaccttcgt catggaccga tggggcgaat acttccgccc
      481 gcactgccgg accgcggacg gcgttgccct cgaggcagtg ccggaaggag gcctcggctt
      541 cttcacctcg tacgaccagg gaggccggcg catcacctat tacggcgaca accacccggt
      601 atacgtcggc aacgtggtca aggccatggc ctcggcccgc gacggctccc gcgaggtgac
      661 ggccctcttc gcccccgagc tggcccggct cgatccggcc gatcaggctg atcgcgaccg
      721 cgccttcgcc gaactcaatg accgcctcga cgacctcctc gtggcgcgcg tcgaggaaaa
      781 ccgccgcctc gcaccgggcg tgatcgagct cgtcctccgc gcgccgatgg cggcgcgccg
      841 cttccggccg gggcagatct tccggctgca gaactacgaa acgcgcgccg accaggtgga
      901 cggccacccg ctgacgatgg aggggctccc ggtcaccggc gcgtgggccg atccggtggc
      961 gggcctcgtc ggcgtggtgg tactcgaaac cggcggctcg gcgcgcctgg ccgcgcttct
     1021 tgtgcccggc gagccggtcg tcctcatggg gccgagcgga gcgccgagcg acatccccga
     1081 aaatgaatcg gtgctcctcg tgggcgccgg cttcggcgtt gccgtgatcc cgtccctggc
     1141 gaaggcgatg cgcgaacgcg ggaaccacgt gactgcggtg gccggtttcc ggagcgcggt
     1201 cgatctcttt taccgcgacg ccatcgaagg cgccaccgac cgggtgatct ggtgcacgga
     1261 ggaaggggcc gccatcgaac cgcgccgccc cggcgaccgc gcggccaccg ggacgccgat
     1321 cgaggggctc caggcctggg cgtctggaga actcagggtg gacgaccccg cgctggcggg
     1381 cacggagcat cttctcgccg tcggtccgga tgccctcctg tcggccttgg ggagcgcgcg
     1441 gcggaacggc cttgaggccc cattcggaac cgcgcgcacg gccgtggcct cgatcaatgc
     1501 gccgatgcag tgcatgatga aggagatctg cgcccagtgc ctgacccggc acgtcgatcc
     1561 ggaaaccgga aaggaatcgt acgtgttctc ctgcttccgc caggatcagc cgctggaccg
     1621 ggtcgatttt gcccacctgg ccgcgcgtct ctcgcagaac gcgacctcgg aaacccttac
     1681 acacctctgg atcgatcgcc tgctggcccg gcgcgcggcc ggatcgggag ccccatgaca
     1741 gccaccgcct ccttcgtcct cgacgtcacc gaacgcgact tcgaggcgca ggtcatcgaa
     1801 aaatcgaaaa gcgtccccgt cgtcgtcgat ttctgggccc cctggtgcgg cccctgccgg
     1861 gcactcggcc ccgtcctcga gaaactggcc gccgaatgga acggcggctt cgtgctcgcc
     1921 aagctcaata ccgacgaggc gccctacatc tcacaggccc tccgcatctc gagcatcccg
     1981 ctggtgatgg ccttccacga aggacaggtg gtcgacgaat tcgtcggcgc cctccccgag
     2041 gcccagatcc gcgagtggct cacgcggctc gcggcgccga tggccgccga cgcgaccact
     2101 gcggaagcca ccgccgccaa cgacaccggc gccgcccgcg ccgacaggct ccgg
//
LOCUS       DASVOD010000599         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_873484, whole genome shotgun sequence.
ACCESSION   DASVOD010000599 DASVOD010000000
VERSION     DASVOD010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873484"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VF720_13190"
     CDS             <1..1234
                     /locus_tag="VF720_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880363.1"
                     /translation="VLAALSPFLVWYSQETRHYALGILAAAWSAITFLDLIDEQSSGG
                     ASAGRGVTAGHGASFTPRLVACYGISLLIGLMSHLATAFLIGAHALVILLWRRNRWKP
                     WLMAVVPALVFFSPWIWVTITRNVNLAHVAAAGPVPEGELLRGESTFSWMGIPYTFFV
                     LLAGYSLGPTLVELHDAPRLATVTPYFAPILLLILGAAGLGLGGIAALRRNRAHLGLV
                     LFCVAVPLIGVTLLSMRNAKVFHPRYVAVALPLVLALLGAGFAWWRERRPAIAWLLVL
                     LIGAPLGLSLANYFTNGRYAREDSRGAARILADRAAPDDIVLAQGAPQLLDWYDHGPA
                     PVVVVYEPWVRDRAALDARLDGWAAGRRHVWLMSVRPWLQDPKLLLRAGLEARWGPGE
                     VHPLNGVTLIRYDRKGAP"
     gene            1231..1701
                     /locus_tag="VF720_13195"
     CDS             1231..1701
                     /locus_tag="VF720_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7880364.1"
                     /translation="MTPSTKGTGSVETGQVEDADGITVIRGGGSARGWNGIQYLQGMS
                     ARNVGSGALSINVATVPPGAIAYAHIHDGFEVMLFIVQGRVRHTYGVGLSRSIDNEAG
                     DFIYIKPGVPHEVFNIGDEPLIAFVARSAADEWDRIVDYPSEFRPMDASGGEKK"
     gene            1741..>2664
                     /locus_tag="VF720_13200"
     CDS             1741..>2664
                     /locus_tag="VF720_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880365.1"
                     /translation="MPRKISEPANPDPATLSRRCFEEFTDLAREAGAALHAAGQVRLG
                     SPGPTGISGKVGEKGKEVERPELDWSWADSRGVLWVSCGCDDFEEGRLCAHLWALIVA
                     MDERNLARLVPGKGPLEVLDDEAADEEEDGEATPAGPLIATRGRKPDNARWYRPSDPS
                     ATRVKPPGGWKHLVAGLDAPPQPRSSYVPQPVKAPSPIGPVWYLLNEQATRSSGQLVI
                     EFHRIAATRRATESPLKPLPLGRADLAALTDAADKDILTILMGADPDYDDPSAAAREG
                     DPQRHARLGVPGPLLSAIVPRLAATGRFGRPD"
BASE COUNT          395 a          914 c          910 g          445 t
ORIGIN      
        1 ggtcctggcc gcgctctcac cctttctcgt ctggtattcg caggagacgc ggcactacgc
       61 gctgggaatc ctggcggcgg cgtggtcggc catcacgttc ctggacctga tcgatgaaca
      121 gtcatcaggg ggcgcctcgg cggggcgggg cgtgacggcc ggacatggcg cgtcgttcac
      181 cccgcgcctc gtggcctgtt acggaatcag cctgctcatc ggcctcatga gccacctggc
      241 taccgcgttc ctgatcgggg cgcacgccct ggtgatattg ctgtggcggc gcaaccgctg
      301 gaagccgtgg ctcatggccg tggtccccgc actcgtcttc ttttccccgt ggatctgggt
      361 gacgatcacc cggaacgtga acctggccca cgtggccgca gcgggaccgg tgccggaagg
      421 agaattgctg cgcggcgaat ccaccttttc ctggatgggg attccctaca cgttcttcgt
      481 gctgctggca ggatattcgc tcggccccac gctggtggaa ctccacgacg caccgcgcct
      541 cgccacggtg accccctact tcgcacccat cctgttattg atactgggtg cggcgggact
      601 cggactgggg gggatcgcgg ccctccgccg caatcgagcc catctaggtc tcgtcctctt
      661 ctgcgtggcc gttccgttga tcggcgtcac gctcctctcg atgcggaacg ccaaggtgtt
      721 ccacccgcgc tacgtggccg tggcgctgcc gctggtgctg gcgctgctcg gcgcgggatt
      781 cgcgtggtgg cgggagaggc gcccggctat cgcatggctc ctcgtcctgc tgatcggcgc
      841 tcccctcggg ctttcactcg ccaactactt caccaatggc cgatatgccc gcgaagacag
      901 ccgcggcgcc gcgcgtatcc tggccgaccg ggccgcgccg gatgacatcg tgctggccca
      961 gggcgccccg cagctcctcg actggtacga tcacgggccc gcgccggtgg tggtggtgta
     1021 tgagccctgg gtgcgcgacc gcgcggcgct cgacgcgcgc ctcgacgggt gggcggccgg
     1081 gcgcaggcac gtgtggctga tgagcgtgcg tccgtggctg caggatccga aactcctgct
     1141 gcgcgccggc ctcgaggccc gctggggtcc gggggaagtg catccgctga acggcgtcac
     1201 cctgatacgt tatgacagga aaggagcccc atgactccgt cgacgaaagg caccggttcc
     1261 gtcgagaccg gccaggtgga agacgcggac ggcatcaccg tcattcgcgg cggcgggagt
     1321 gcgcgcgggt ggaatgggat ccagtatctg caggggatgt cggcgcgtaa cgtcgggtcg
     1381 ggcgcgctgt cgatcaacgt ggccacggtg ccgcccggcg cgatcgccta cgcccacatc
     1441 cacgacggct tcgaggtgat gctgttcatc gttcagggtc gcgtgcggca cacctacgga
     1501 gtggggcttt cacggtcgat cgacaacgag gcgggcgatt tcatctacat caagcccggc
     1561 gtgccgcacg aggtgttcaa tatcggggat gagccgctga ttgccttcgt ggcgcgatcg
     1621 gcggccgacg agtgggaccg gatcgtcgac tacccctctg aattccgccc tatggatgcc
     1681 tccgggggcg aaaagaaata gggtcgaccc aatccgcgcg gagcgggtta caattgccgg
     1741 atgccccgaa agatttccga acccgccaat cccgatccgg ccacgctctc ccgccgctgt
     1801 ttcgaggaat tcaccgacct ggcccgagag gccggcgctg ccctgcatgc tgccgggcag
     1861 gtccgactcg gctcgcccgg ccccaccggc atcagcggca aggtcggcga gaagggaaag
     1921 gaagtcgagc gccccgagct ggactggtcg tgggcggata gccgcggcgt cctctgggtg
     1981 tcgtgcggct gcgacgactt cgaggaaggg cgtctctgcg cccacctctg ggcactcatc
     2041 gtggccatgg acgagcgcaa cctggcacgg ctcgtgcccg gcaagggtcc gctcgaagtg
     2101 ctcgacgacg aagccgccga cgaagaggaa gatggcgagg ccacgccggc cggtcccctg
     2161 atcgcaacga gggggcgcaa gcctgacaac gcgcgctggt atcggccgag cgatccctct
     2221 gccacccgcg tgaaaccgcc gggcggctgg aagcacctgg tggccggcct cgacgccccg
     2281 ccgcagcccc gctccagcta tgtgccgcag ccggtcaagg cgccgtcacc gatcgggccg
     2341 gtctggtatc tcctgaacga gcaggcaacg cgaagctccg gacagctcgt catcgaattc
     2401 caccggatcg cggccacgcg gcgggccacc gagtcaccgc tcaagccgct gccgttgggg
     2461 cgcgccgatc tcgccgcgct gaccgatgcg gccgacaagg acatcctcac gatcctgatg
     2521 ggcgccgacc cggactacga cgacccctcg gccgccgccc gtgagggaga tccccagcgt
     2581 cacgcgcggc tcggcgttcc cgggccgttg ctctcggcca tcgtgccgcg cctggcggca
     2641 accggtcgct tcggtcgtcc cgac
//
LOCUS       DASVOD010000600         3093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_612264, whole genome shotgun sequence.
ACCESSION   DASVOD010000600 DASVOD010000000
VERSION     DASVOD010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612264"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VF720_13205"
     CDS             <1..532
                     /locus_tag="VF720_13205"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7880366.1"
                     /translation="LTLVEACQGMADDTIVATSEAQEVELKRLRHALPATMNERGRAA
                     RAAGGMKLGTDWAVPLESVGPMIETATRLADDTFGGSYIRYGHIGNGHPHFNLLAENA
                     DGLAKARRAIHAMCLEAVRLGGTVTAEHGVGKMKREYVALQYPAWVVEAMTSVKRTLD
                     PSGILAPGNIFEPKPS"
     gene            550..2247
                     /locus_tag="VF720_13210"
     CDS             550..2247
                     /locus_tag="VF720_13210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7880367.1"
                     /translation="MTESIRGRAAWVAAILPAAVLAIGAWCHRWVCEDAFICFRVADQ
                     ILAGNGPVFNAGERVEAYTSPAWMALLTLWQALGGRIEEGSLVLGILAAGVGVVTAVL
                     AAARRHAPRAVVPFGALVLVVTTAVWDFAASGLEMGLVLAWLGTSYALLSRAASDRRA
                     SGAGAFAWFGIGILIRPDLGLFAIGFLLIALFLRRGMTNTQAGSIASSLVAFGAVPLL
                     YLLFRMGYFAAWMSNSSIAKEPGLAHGTAGAYYLLDFVHVYRLWIVAPLVLLLAWLAR
                     SSRRIPLVGVEATFFALGVLHAVYVARVGGDFMHGRFVLPAYMAMLIPFFVLPVAPLR
                     RPAAAVGALALYGWCAASAFVFGPHYRYNRRGVANERLLYIELAGRPHPVRLEDYDDT
                     IFGREGRALRKEAELGGRRLLVALTDDPAGGYLPADARRVAPLSASLHPRITVAAPAW
                     NIGIIGRHAGTRAFVADRLGLADPIGSRLRLKTPGRPGHEKLLPLAVVEARLTDPQPG
                     ERAEVTGARAMLAGPEARELIEATSAPMSVPRFFSNVLSAVRLTSLRFDFERLVPPR"
     gene            complement(2290..2589)
                     /locus_tag="VF720_13215"
     CDS             complement(2290..2589)
                     /locus_tag="VF720_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011377556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HEX7880368.1"
                     /translation="MTLPPCLFKAVAGLPCPSCGLTRSGLAMGQGDWVAAFRFHPLGP
                     LFVVEALAVGVFLAVRRSRGMSLKPAQPIVDRWIVANVTLLLGVWLARLASGWRG"
     gene            complement(2586..>3093)
                     /locus_tag="VF720_13220"
     CDS             complement(2586..>3093)
                     /locus_tag="VF720_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HEX7880369.1"
                     /translation="LAVFSQDVLTRGALAVLTTGNGRSSLSKALDAESGVLDGAFREP
                     QDVAGRPEAYWINRALTLDLMNLLPDGTFRPDQPVTRQVLALWAEEMIARLRGNEKVF
                     SLYRGSESPFTDMKSTHYAYNAARIVVDLKMVTPEGGAFEPDRPVTMDEGVAAIQRFV
                     PALAGAGR"
BASE COUNT          449 a         1098 c         1041 g          505 t
ORIGIN      
        1 gctcacgctg gtcgaagcct gccaggggat ggccgacgac acgatcgtgg ccacgagcga
       61 ggcgcaggaa gtcgagctga agcgcctgcg tcacgccctt cccgccacga tgaacgaacg
      121 agggcgcgcg gcccgcgcgg ccggtgggat gaagctcggc accgactggg ccgtgccgct
      181 ggaatcggtc ggccccatga tcgagaccgc cacgcgcctc gccgacgaca cgttcggcgg
      241 ctcctacatc cgctacggcc atatcggaaa cggccatccc cacttcaacc tcctggccga
      301 aaatgccgac gggctggcga aggcgcggcg cgccatccac gccatgtgcc tcgaggccgt
      361 gcgactgggc ggcaccgtca ccgccgagca cggggtcgga aagatgaagc gcgagtacgt
      421 ggcgctgcag tacccggcct gggtggtgga ggccatgacc agcgtgaagc gcacgctcga
      481 tccgtctgga atcctggcgc cggggaacat cttcgagcca aagccgtcct gacctgaccc
      541 gggaggccga tgaccgagtc gattcgtggc cgtgccgcct gggtggcggc catccttccg
      601 gcggccgtcc tcgcgatcgg cgcgtggtgc caccgctggg tctgcgagga tgcgttcatc
      661 tgctttcgtg tggccgacca gatcctcgcg gggaacggcc cggtcttcaa cgccggcgag
      721 cgggtcgagg cctacacgag cccggcctgg atggcgctcc tcacgctgtg gcaggcgctt
      781 ggtggccgga tcgaggaagg ctcgcttgtc ctcgggattc tcgccgccgg cgttggcgtc
      841 gtgacggccg tcctggctgc ggcgcggcga catgcgccac gcgccgtcgt tccgttcggc
      901 gcactggtcc tggtcgtgac caccgccgtg tgggacttcg cggcctccgg cctcgagatg
      961 gggctggtgc tcgcgtggct cggaacgtcg tatgcgctgc tctcacgggc cgcctccgat
     1021 cgacgcgcgt cgggcgcggg cgcctttgcc tggttcggga tcggcatcct catccgaccc
     1081 gacctcgggc tctttgcgat cggatttctg ctgatcgcgt tgttcctccg gcgaggaatg
     1141 acaaacacgc aagcgggctc aatagcgtcg tctctcgtcg cattcggcgc cgtccctctc
     1201 ctctacctcc tcttccggat ggggtacttc gccgcgtgga tgtcgaattc ctcgatcgcg
     1261 aaggagccgg gactcgcgca tggaacggcc ggcgcctact acctgctcga tttcgtccac
     1321 gtctatcgac tctggatcgt cgcccccctc gtacttctcc tggcatggct tgcccggtcg
     1381 agccggcgca tcccgctcgt tggagtcgag gccaccttct tcgccctcgg cgtcctgcat
     1441 gccgtctacg tggcccgcgt cggcggcgac ttcatgcacg gacgattcgt cctccccgcc
     1501 tacatggcca tgctgatccc gttcttcgtc ctgccggtcg cgcccctgcg gaggccggcg
     1561 gccgcggtcg gggcgctggc gctgtacggc tggtgcgccg ccagcgcctt cgtgttcggg
     1621 ccccactatc gatacaaccg tcgcggcgtc gcgaacgagc gtcttctcta catcgagctg
     1681 gcgggccgtc cccatcccgt gcgtctggag gattacgacg acaccatctt cggccgcgaa
     1741 ggacgcgccc tgcgcaagga agcggagctg ggtggacggc gcctgctcgt ggcgctcacc
     1801 gatgacccgg caggcggtta ccttccggcc gatgcccgtc gcgtggcacc gctctcggca
     1861 tccctccacc cgcggatcac cgtggccgcg ccggcctgga acatcggcat catcgggcgc
     1921 cacgccggaa cgcgcgcctt cgtggcggac cggctcggcc tggctgatcc gatcggcagc
     1981 cggctccggt tgaaaacgcc cggtcggccg ggacacgaga agctcctgcc attggccgtg
     2041 gtggaggcgc ggctcacgga tccccaaccg ggggaacggg cggaggtgac cggggcacgg
     2101 gcgatgctgg caggtcccga ggcccgcgaa ctgatcgagg cgacttcggc accgatgagc
     2161 gttccgcgat ttttctcgaa cgtcctctcg gcggtgcgcc tcacgtcgct tcgcttcgat
     2221 ttcgaaaggc tggtcccgcc gcgctgatgc cgccccgcgt ccgatcgccg gcgttggcga
     2281 tgggttacct cacccccgcc aaccgctggc cagtcgcgcc agccacacgc cgagcagcag
     2341 cgtcacattc gcgacgatcc accgatccac gatcggctga gccggcttga gcgacatgcc
     2401 gcgcgagcgt cgcacggcga gaaacacgcc gaccgcgagc gcctcgacca cgaaaagcgg
     2461 tccgagcgga tgaaagcgga acgcggccac ccaatcaccc tgccccatcg ccaatcccga
     2521 ccgcgtgagg ccgcacgacg gacagggcaa ccccgccacc gccttgaaga ggcagggcgg
     2581 gagcgtcacc gcccggcgcc ggccagtgcc ggaacgaagc gctggatggc ggccacgccc
     2641 tcgtccatcg tgaccgggcg gtcgggctcg aaggcgcctc cttcgggcgt caccatcttc
     2701 aagtccacca cgatccgggc cgcgttgtac gcgtaatgcg tcgacttcat gtcggtgaag
     2761 gggctctccg aaccgcggta aaggctgaag accttctcgt tgccgcgcag ccgggcgatc
     2821 atctcctcgg cccagagcgc cagcacctgc cgggtgaccg gctggtccgg gcggaaggtg
     2881 ccgtcgggga gcaggttcat gaggtcgagc gtcagcgcgc ggttgatcca gtacgcctcg
     2941 gggcggccgg ccacgtcctg cggctcacgg aacgcaccgt cgagcacccc cgattcggca
     3001 tcgagcgcct tcgaaagaga gctcctgcca ttgcccgtcg tgaggaccgc cagtgcgcca
     3061 cgcgtgagca cgtcctggga gaagacggcc agg
//
LOCUS       DASVOD010000601         2040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1034148, whole genome shotgun sequence.
ACCESSION   DASVOD010000601 DASVOD010000000
VERSION     DASVOD010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034148"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VF720_13225"
     CDS             complement(<1..1101)
                     /locus_tag="VF720_13225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX7880370.1"
                     /translation="MHGSSTAGPRRSFPGALLIPVIGLVFAFLFAAQNVYRGVILGEP
                     IGWRDMLANTLPRFLAYSLLAPLVGWLVERAPVGGANAWRALGVQIAGGVTFAIVHSA
                     LIAMYFAIFHLYPAGETLVGAFRRLMVVHFGMDLIVFAGITGAYHAQRYVREVRERDR
                     RAAALEQQLTAARLDALRSQLNPHFLFNTLNVTSAMALAGERDRVVETLGSLGQLLRV
                     SLDPTLPQLVPLEREMALLDAYLDIQRVRFGERLTIDWEVAADARAVPVPSMLLQPLV
                     ENALQHGIAERPGPGRVGIHAARREGRLMLRIEDDGPGFNGADQPKGHGVGLGNTRAR
                     LAELYGDRASLRTGNRPDGGAFVAIELPAEEVT"
     gene            complement(1215..>2040)
                     /locus_tag="VF720_13230"
     CDS             complement(1215..>2040)
                     /locus_tag="VF720_13230"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX7880371.1"
                     /translation="VTQGFIGATRDGVTTTIGRGGSDLTASMVGAALGADEVQIWTDV
                     DGIMTTDPRVVPEARLIEEIAFAEAAELAYFGAKVLHPSTLRPAMAHNVPVRVLNSRK
                     PEGRGTRVVESLPAAGRPITAIAFKRGITVLNVTSDRMLMAHGFLARLFDVFSRHETA
                     VDMVTTSEVSVSMTLDDTRHLDAIRDALASFGEVVVDADQAIICLVGESLKSTPGIVP
                     RIFAAVNTMPVSMISHGASAINLSFVVPGARAEEAVRSLHRALLELPAVPVSRLPA"
BASE COUNT          336 a          707 c          714 g          283 t
ORIGIN      
        1 ggtcacctct tcggcgggca gctcgatggc cacgaacgcg ccgccatccg gccggttgcc
       61 ggtgcgcagg gacgcccggt cgccatagag ttcggcaagg cgggcccgcg tgttgccgag
      121 ccccacgccg tgtcccttgg gctggtcggc cccgttgaag cccggaccgt cgtcctcgat
      181 gcgcagcatg agccgccctt cgcgccgcgc ggcatggatg cccacccgcc ccggccccgg
      241 acgctcggcg atgccgtgct ggagcgcgtt ctcgaccagc ggctggagaa gcatgctcgg
      301 caccggtacg gcccgcgcgt ccgccgccac ctcccagtcg atcgtcaggc gctcgccgaa
      361 gcggacgcgc tggatgtcca ggtaggcgtc cagcagcgcc atctcccgtt cgagcggcac
      421 gagctgcggc agggttggat cgagcgagac gcgcagcaac tgcccgaggg agccgagcgt
      481 ttccaccacg cgatcgcgct cgccggccag cgccatggcg gaggtgacgt tcagtgtgtt
      541 gaacaggaag tgcgggttga gctgggagcg cagggcatcg agcctcgccg cggtgagctg
      601 ctgctcgagt gccgcggccc ggcgatcgcg ctcacgaacc tcgcgcacgt agcgctgggc
      661 atggtacgca ccggtgatgc cggcgaagac gatgagatcc atgccgaagt gcaccaccat
      721 gaggcgtcgg aaggcgccga ccagcgtctc ccccgccggg tagaggtgga agatggcgaa
      781 gtacatcgcg atcagggccg aatgaacgat cgcgaaggtc actcccccgg cgatctgcac
      841 gccgagcgcc cgccaggcgt tggcaccgcc cacgggcgcc cgttccacca gccagccgac
      901 cagcggggcc agcaggctgt aggccaggaa gcggggcagc gtgttggcca gcatgtcgcg
      961 ccagccgatc ggttccccga gaatgacgcc gcggtacacg ttctgcgccg cgaagaggaa
     1021 ggcgaagacg aggccgataa ccgggatgag gagtgccccg ggaaacgacc ggcgggggcc
     1081 cgccgtggat gagccgtgca tgccgcattg tggccaagcc cggttctatt gggaaggcgc
     1141 cgaatgccgg attgctcagt tcatcagcga aagctcttgg gaggcgccgg aacgggaagg
     1201 gggcgccgtc cggcctaagc cggcagtcgg gagaccggga cggccggcag ttccagcaac
     1261 gcccggtgca acgaccgcac cgcctcctcc gcccgggccc cgggcaccac gaagctcagg
     1321 ttgatggccg acgccccgtg cgaaatcatc gataccggca tcgtgtttac cgcggcaaag
     1381 atgcgcggca cgatgccggg cgtggacttc aggctctccc caaccaggca gatgatggcc
     1441 tggtcggcat cgacgacgac ctcgccgaac gaagccagcg cgtcacgaat ggcatcgagg
     1501 tgccgcgtgt cgtccagcgt catcgacacg ctcacctcgg acgtggtcac catgtccacg
     1561 gcggtttcgt gccggctgaa cacgtcgaac agccgggcca ggaagccgtg ggccatgagc
     1621 atgcggtccg acgtgacgtt gagcaccgtg atgccccgct tgaacgcaat ggccgtaatc
     1681 ggccgccccg ccgccggcag cgactcgacg acgcgcgtcc cccgcccctc cggcttccgc
     1741 gaattgagca cccgaaccgg cacgttgtgc gccatggccg gccgcagcgt gctcggatgg
     1801 agcaccttcg ccccgaagta ggcgagctcg gccgcctcgg cgaaggcgat ctcctcgatc
     1861 aaccgtgcct ccggcaccac gcgcgggtcg gtggtcatga tgccgtccac gtcggtccag
     1921 atctgcactt cgtcggcccc gagcgcggct ccgaccatcg aggccgtgag atccgagccg
     1981 ccgcgcccga tggtggtggt gacgccgtcg cgggtggcgc cgatgaagcc ttgcgtgacg
//
LOCUS       DASVOD010000602         2067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_41809, whole genome shotgun sequence.
ACCESSION   DASVOD010000602 DASVOD010000000
VERSION     DASVOD010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41809"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..998
                     /locus_tag="VF720_13235"
     CDS             495..998
                     /locus_tag="VF720_13235"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880372.1"
                     /translation="MRDGQYQILAICTGNICRSPMAEGILRARLSARAAEFATVGSAG
                     TSAMAGIPASAHSVEVCRQHEMDISGHRSRPLTGDMIDEADLILVMEEHHRQAVLRMR
                     RDAADRTHLVSQFAADGPGAAVGVDDPIGHDLDTYLAVFRRLDEYITRAMPRIEASVA
                     GSSRRLS"
     gene            995..1960
                     /locus_tag="VF720_13240"
     CDS             995..1960
                     /locus_tag="VF720_13240"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX7880373.1"
                     /translation="MSHGSTGYKPGAKIWMDGKLVDWADAKIHIVSHVIQYGSGVFEG
                     IRVYANPKGNYAFRLKEHLNRLLGSAKIYRMDSGMTLDQLTSACLETAAVSGFDACYI
                     RPLIYRGFGNVGVNPLGCPINVSIPVWQWGAYLGADGVEQGIDVKVSSWCRLANNTMP
                     ALAKATSNYMNAQLIKAEALLDGYAEGIGLNTDGTVSEGSGENIFLVINGRLQTPALS
                     DSILSGITRNAVMTLAADLGIEVREAPVPREMLYLADEVFFCGTAAEITPIKSIDKIA
                     IGAGRRGPVTEKLQKAFFEIVRGEIPDRHGWLTQVPAMAGVASAR"
BASE COUNT          354 a          695 c          672 g          346 t
ORIGIN      
        1 actaacccac tctactccgc gctacgtaat tccttggatt tcaagaatct ggcccgctcc
       61 cttgacgttg attctcaagg cgaggccgga catccctggc cgcgcccccg atggctcggt
      121 ggcgctgcgc tgccccgccc aggcatttac gcgggcgctt acccgggagg tggcaggtgt
      181 catggcctca acaagtgcca atcgaatggg ggagccaccc gtcggagacg ctcgggaggc
      241 gctggcccgt ttcgggattg ggccggaggg ggtggatgca gtgctcgccg atcgcaggcc
      301 gaccgcaccc ggtgctggtc caattccagc aagtttggcc tcgacgatca ttgactgccg
      361 ggaggcgtcc ccgcggattg tccgcatggg agccgttccg gccgaacggc tcggaatggc
      421 ctcgggctga cctggccgtt gcctgaccca cccctgcctg acccacttcc accggggcct
      481 gttagaatca caggatgcgt gacggccaat accagatcct cgccatctgc accgggaaca
      541 tctgccgcag tccgatggcg gaagggatcc tccgggcgcg cctgtcggcg cgggcagccg
      601 aattcgccac ggtgggctcg gccggcacct cggccatggc cggcatcccg gcttcggccc
      661 attcggtgga ggtgtgccgg caacacgaga tggacatctc cggacaccgc tcgcgcccgc
      721 tcaccggcga catgatcgac gaggccgacc tgatcctcgt catggaagag caccaccgcc
      781 aggcggtcct ccggatgcgg cgcgatgcgg ccgaccggac ccacctggtg tcgcagttcg
      841 cggccgatgg cccgggtgcg gcggtcgggg tggacgaccc gatcggtcac gatcttgata
      901 cctacctggc ggtcttccgc cggctcgacg agtacatcac ccgcgccatg ccccggatcg
      961 aggcatccgt ggccggttcc tcacggaggt tgtcatgagt cacggttcca cgggttacaa
     1021 gccgggcgcg aagatctgga tggacggcaa gctggtcgac tgggcggacg cgaagatcca
     1081 catcgtgagc cacgtcatcc agtacggctc cggcgtgttc gaggggatcc gcgtgtacgc
     1141 caacccgaag gggaactacg ccttccgttt gaaagagcac ctgaaccggc tgctcggctc
     1201 ggccaagatc taccggatgg attcggggat gaccctggac cagctgacga gcgcatgcct
     1261 cgaaacggcc gctgtctccg gcttcgacgc ctgctacatc cgcccgctca tctaccgcgg
     1321 attcggcaac gtcggcgtga acccgctcgg ctgcccgatc aacgtatcga ttccggtgtg
     1381 gcagtggggc gcctacctcg gcgccgacgg ggtcgagcag gggatcgacg tgaaggtgtc
     1441 gtcgtggtgc cgcctggcca acaacacgat gccggcgctg gccaaggcca ccagcaacta
     1501 catgaacgcc cagctcatca aggccgaagc gctcctcgac ggctacgccg agggaatcgg
     1561 cctcaacacc gacggcacgg taagcgaagg ctcgggcgag aacatcttcc tcgtgatcaa
     1621 cggtcggctg cagacgcctg ctttgtcgga ctcgatcctt tccggcatca cgcgcaacgc
     1681 cgtcatgacg ctggccgccg acctcggcat cgaagtccgc gaggcgcccg tcccgcgcga
     1741 gatgctctac ctggctgacg aagtgttttt ctgcggcacc gccgcggaaa tcacgccgat
     1801 caagtcgatc gacaagatcg ccatcggggc gggccgccgc ggtccggtca cggagaaact
     1861 gcagaaggcc ttcttcgaga tcgtgcgcgg cgagataccg gatcggcatg ggtggctgac
     1921 gcaggtgccg gcgatggcgg gagtggcctc cgcgaggtag cgcccgccca ttgggattcc
     1981 acttccgaac ctgatagggt gccctgccat ggactggact cttgagaacc tcgccgccca
     2041 ggaccccgcc atcgcggacg tcctcaa
//
LOCUS       DASVOD010000603         2985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_145223, whole genome shotgun sequence.
ACCESSION   DASVOD010000603 DASVOD010000000
VERSION     DASVOD010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145223"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF720_13245"
     CDS             <1..351
                     /locus_tag="VF720_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880374.1"
                     /translation="FRSEGVRNTWQLPVNTDPARWLPEQVESTGFFVEGAWRALPIVT
                     LGGRYDRIDFDEIESPTGRRDTWDANVRRWEGAVAWHPSRDWEIKVSYQDWMYPDDEH
                     LDADMAGFQLRMEF"
     gene            351..1019
                     /locus_tag="VF720_13250"
     CDS             351..1019
                     /locus_tag="VF720_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880375.1"
                     /translation="MKPLRGWMAKALLVLGALSALATLASAATVNGRINGVLPPLRKS
                     PDLYGKYRRGQPAPDLPNPLLVVLIPADGSAPPVVTKHPVIDQKDERFQPSALVVQTG
                     TTVDFLNSDPFYHNVFSLSSPRKFDLGRYRKGSSRPVTFEKPGLVRLFCDIHPSMLGY
                     VLVSPSPWGAAASPEGNFAITDVPPGTYDVMVWHEPLTDPIRIDTIAIAAETAPLELM
                     LSGR"
     gene            1024..2976
                     /locus_tag="VF720_13255"
     CDS             1024..2976
                     /locus_tag="VF720_13255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880376.1"
                     /translation="MSPFRLRHRILLVLCAVLLPFAALTFLVVEQRLRGEAVKGLNSD
                     LASARESFAELRRQWQTELTQSAALVAELPYFKAATAVYDPDAPRPAQMEQIATIEPV
                     ARDIMSHLGLDFLALTDSRDNLVLAFRGEKRLQEPEFGDAFRRLGQEVVERGLAGGSL
                     DVDGEIEQVIMIPVTAGGVEFGRLAIGRRLDDELAARVRRMTRTEVAILGTSKPLARC
                     CGSADGPRENDLFELVRHSPQLGLVNGAPVELKLAHERFLTLWTPIDDPTGRTIGAYV
                     LQASLDDALSVVDSVRQTMLLLWLVALLTAILLSFRAARQLTSPIDGLSQAARRLSGG
                     DFAARVAEEGAEELRDLARTFNQMSAGLEESHRQLGEYNLRLTERTAELEESHHHLLQ
                     STQMLEQSNRELREMHAQLIQAGKMAAFGELGAGVAHELSQPLTSLKGFAQLALVRMS
                     DDDPARSHLSRIIQSCDHMTRIVSSLKNFARMSQFDQRPISLNGVIEETTVFLAAQFR
                     KHRVRINMELATDLPEVIGDANQLQQVVTNLMANARDAMDGRPDAAVTLSTGTRFGGR
                     WIVARVRDNGPGVPAELRSKIFRTFFTTKDAGKGTGLGLSISRGIIQDHGGRLGVSSS
                     AAGGAVFHIILPTPAVAAERQKNVAA"
BASE COUNT          499 a         1041 c          954 g          491 t
ORIGIN      
        1 ttccggagcg aaggtgtccg caacacctgg cagctgccgg tcaacaccga cccggcccgc
       61 tggctccccg agcaggtgga atcgaccgga ttcttcgtag aaggcgcctg gcgcgccctt
      121 cccatcgtga cgctcggcgg ccgctacgac cggatcgact tcgacgagat cgagagtccc
      181 accggccgcc gtgacacctg ggacgccaac gtccgccgct gggaaggcgc cgtggcctgg
      241 cacccctctc gtgactggga gatcaaggtg tcgtaccagg actggatgta tcctgacgac
      301 gagcatctcg acgccgacat ggccggcttc cagctccgga tggagttctg atgaagccac
      361 ttcgtggctg gatggcaaag gccctgctgg tcctcggcgc tctatccgca ctggccacac
      421 tggccagcgc cgctaccgtc aacgggcgca tcaacggcgt cctgcccccg ctccgcaaga
      481 gccctgacct gtacggcaag tatcgccgcg gacagccggc gcccgatctt ccgaacccgc
      541 tcctcgtggt gctgataccg gcggatggct ccgcgccgcc tgtggtgacg aagcacccgg
      601 tcatcgacca gaaggacgag cgcttccagc cgagcgcgct ggtcgtgcag accggcacca
      661 cggtggactt cctcaacagc gatcccttct atcacaacgt cttctcgctc tcctcgccac
      721 gcaagttcga tctcggccgt taccgcaagg ggagttcgcg gccggtcacc ttcgaaaagc
      781 ccggcctcgt gcggctcttc tgcgacatcc acccgagcat gctcggatac gtgctggtgt
      841 ccccctcccc gtggggcgcg gccgcatcgc ccgaaggaaa ctttgccatc accgacgttc
      901 cgccgggcac gtatgacgtg atggtgtggc acgagcccct cactgacccc atcaggatcg
      961 atacgatcgc gatcgcggcc gagacagccc ccctggaatt gatgctttcc ggtcgatgac
     1021 accgtgagcc ccttccgcct gcgccaccgc atcctgctcg tgctgtgtgc cgttctgctt
     1081 cccttcgcgg cgctcacctt cctcgtcgtc gagcagcggc ttcgtggcga agcggtgaag
     1141 gggttgaaca gcgacctggc ctcggcacgt gagtcgttcg ccgaacttcg tcgccagtgg
     1201 cagacggagc tgacccagtc ggctgcgctg gtggccgagc ttccgtattt caaggccgcg
     1261 accgccgtgt acgacccgga cgcaccgcgt cccgcccaga tggagcagat cgccacgatc
     1321 gaaccggtgg cgcgcgacat catgagtcac ctggggctcg acttcctggc attgaccgac
     1381 tcgcgcgaca acctggtcct ggcgttccgg ggcgaaaaac gcctccagga gcccgaattc
     1441 ggcgatgcct tccggcgact cggacaggag gtcgtggagc gaggcctggc cggcggctcg
     1501 ctcgacgtgg atggcgagat cgaacaggtc atcatgatcc ccgtgacggc cggcggcgtg
     1561 gagttcggac gattggccat cggccgccgc ctggacgacg agctggcggc gcgtgtgcgg
     1621 cgcatgacgc gtacggaggt tgccatcctc ggcacgagca agcctctggc tcgctgctgc
     1681 ggctcggctg atgggccgcg cgagaacgat ctcttcgaac tggtgcggca ttcgccccag
     1741 ctcggcctcg tcaacggcgc gcccgtcgaa ctcaagctcg cgcacgaacg attcctgacc
     1801 ctctggaccc ccatcgacga tccgaccggg cggacgatcg gtgcgtacgt gctgcaggct
     1861 tcgctggacg acgccctctc ggtggtcgat tcggtgcgcc agaccatgct gctcctctgg
     1921 ctggtggccc tcctgaccgc catcctcctg tcgttccggg cggcgcggca gctcacgagc
     1981 cccatcgacg gtctgtcgca ggcggctcgc cgcctgtcgg ggggcgactt tgcggcgcgc
     2041 gtggcggagg aaggggccga ggagctgcgc gacctggccc gcacgtttaa ccagatgagc
     2101 gccggactcg aagagagtca ccgccaactc ggggaataca acctgcgcct gaccgagcgg
     2161 accgcggagc tcgaggagag ccatcatcac ctcctccaga gcacccagat gctcgagcag
     2221 agcaaccggg aactccgcga gatgcacgcc cagctcatcc aggccggcaa gatggccgcc
     2281 ttcggcgagc tgggggccgg cgtggcccat gaactctcgc agcctctgac cagcctcaag
     2341 ggatttgccc agctggcgct cgtccggatg tccgacgacg accccgcccg ctcgcacctc
     2401 tcacgcatca tccagtcgtg cgatcacatg acgcggatcg tctcgtcgct caagaacttc
     2461 gcccggatga gccagttcga ccagaggccg atcagcctga acggcgtcat cgaggagacg
     2521 acggtcttcc tggccgccca gttcaggaag catcgcgtac gaatcaacat ggagctggcg
     2581 accgacctcc ccgaggtgat cggcgatgcc aaccagctgc agcaggtggt gaccaacctc
     2641 atggccaacg cccgcgacgc aatggatggc cggccggacg ccgccgtgac gttgagcacc
     2701 ggtacacgct tcggcggccg ctggatcgtg gcccgcgtac gggacaacgg accgggcgtt
     2761 ccggcggagc tccgttcgaa gatcttccga accttcttca ccaccaagga tgccgggaag
     2821 gggaccgggc tcgggttgtc gatcagccgc ggcatcatcc aggaccatgg tggccggctt
     2881 ggcgtgagct cgagcgcggc cggcggcgcg gtcttccaca tcatcctgcc gacgccggca
     2941 gtggccgccg aacgacagaa gaacgtggcc gcgtagagcc agtat
//
LOCUS       DASVOD010000604         3313 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_457288, whole genome shotgun sequence.
ACCESSION   DASVOD010000604 DASVOD010000000
VERSION     DASVOD010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457288"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /gene="ftsH"
                     /locus_tag="VF720_13260"
     CDS             complement(<1..1909)
                     /gene="ftsH"
                     /locus_tag="VF720_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX7880377.1"
                     /translation="MMTLFIVLAVHYYRLTKPQEQAITYTTFMEQVDKGNIKKIAFRK
                     DHAHGELGKAEMLPVGDHPASVIFFQVILPFENSKELTEKIQSLNPAADIVGDREETN
                     WLQFVLTYLPVILILAFWLFFIRQMQSGGSTALKFGKSRAKLVLESNPKITFKDVAGA
                     DEAKEELQEVIEFLREPKKFQALGGRIPKGALLLGPPGTGKTLLARAVAGEAGVPFFS
                     MSGSDFVEMFVGVGASRVRDLFEQGKRNAPCIIFIDEIDAVGRHRGAGLGGGHDEREQ
                     TLNQLLVEMDGFESNDGVILIAATNRPDVLDPALMRPGRFDRQIVVDRPDAKGREGIL
                     KVHTRKIPLDETVDVITLARGTPGLAGAELANMVNEAALLAARRNHRKVSMRDFEDAR
                     DKVMMGPERKSMILSDEEKRMTAYHEAGHVLVGKYIPHGDPIHKVTIIPRGRALGVTH
                     YLPLDDRHTYSREYCLGVLARMMGGRAADELIYGQKTTGAGNDIEQATGLARKMVCEW
                     GMSEDLGPLTYGKREEMVFLGREISSHKDYSEATAVLIDREVRSLVDGANERAKNILT
                     DNRSKLDALASALLEREILDAHEIDAVLRGESLPPLNNRSAPPPDAPAAPAADPGAER
                     KPKTTFGGPEPA"
     gene            complement(2301..>3313)
                     /gene="tilS"
                     /locus_tag="VF720_13265"
     CDS             complement(2301..>3313)
                     /gene="tilS"
                     /locus_tag="VF720_13265"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEX7880378.1"
                     /translation="VGPRPFLRPLLGLERAALREWLAAREFTWREDASNDDPVFTRNR
                     VRRELMPILKEHFNPQAVKALVRTASLQQEIDQLLKRLSAAHLDGHARVDVDGGRVIL
                     DRTALLAEPPAVVRAVLRAAGERIAPERLSWGQVHLEGVMGGLIQRGEARWVFPSGMI
                     LVKSGDELTLERPRPAGQIEPATPEPVEVPLVAGTEVPWGEGCLRISRVSLESGGARA
                     LSLDPGPRAAFFNADGLPPRLLLRNIRTGERMSLFGAGQHRKVSDILIDARVPLGARR
                     GVPVLAEPLPPGDGPTDEALWLVGVARSAAATITEATHHLLLAEWFGPLPGAGRKALN
                     DN"
BASE COUNT          483 a         1083 c         1168 g          579 t
ORIGIN      
        1 ccgccggttc gggaccaccg aaagtggtct tcggcttccg ctcggcgccc gggtcggcgg
       61 ccggggccgc cggggcgtcg ggcggcgggg ccgaccggtt gttgagtggc ggcagcgact
      121 cgccgcgaag gacggcgtcg atctcgtggg cgtcgaggat ctcccgttcg agaagggcgc
      181 tggccagggc gtcgagcttg ctccggttgt cggtgaggat gttcttggcg cgttcgttgg
      241 cgccgtcaac cagagagcgg acctcgcggt cgatgagcac cgcggtggct tcgctgtaat
      301 ccttgtggct ggagatttcg cggccgagga acaccatctc ctcgcgcttg ccgtaggtga
      361 gcgggcccag gtcctcggac atgccccact cgcacaccat cttccgcgcc agtccggtgg
      421 cctgctcgat gtcgttgccg gcgccggtgg tcttctgtcc gtagatgagc tcgtccgcgg
      481 cgcggccccc catcatgcgg gccaggacgc cgaggcagta ttcacggctg taggtatggc
      541 ggtcgtcgag cggcaggtag tgcgtgacgc cgagcgcccg gccgcgcggg atgatcgtga
      601 ccttgtggat cggatcgccg tgcgggatgt acttgccgac cagcacgtga cccgcctcgt
      661 ggtacgcggt catccgcttc tcttcgtcgg agaggatcat gctcttccgc tcggggccca
      721 tcatgacctt gtcgcgggcg tcctcgaagt cgcgcatgct caccttgcga tggttgcggc
      781 gggcggccag gagcgccgcc tcgttgacca tgttggccag ttcggcgccg gccaggccgg
      841 gcgttccacg ggccagcgtg atgacatcca ccgtctcgtc gagcgggatc ttccgcgtgt
      901 gcaccttgag gatcccctcg cgccccttgg cgtccgggcg atccaccacg atctggcggt
      961 cgaagcggcc cgggcgcatg agcgccgggt cgagcacgtc gggacggttg gtggcggcga
     1021 tcaggatgac gccgtcgttc gattcgaagc cgtccatctc gacgaggagc tggttcagcg
     1081 tctgctcgcg ctcgtcgtgg ccgccgccca ggccggcccc gcgatggcgg ccgacggcgt
     1141 cgatctcgtc gatgaagatg atgcaggggg cgttccgctt cccctgctcg aaaagatcac
     1201 gcacgcggct cgcgccgacg ccgacgaaca tctcgacgaa gtcggagccg gacatcgaga
     1261 agaacggcac gccggcttca ccggccacgg cccgcgccag gagcgtcttg ccggtgccgg
     1321 gagggccgag gaggagggca cccttgggga tacgcccgcc gagcgcctgg aatttcttgg
     1381 gctcgcgcag gaattcgatg acctcctgca gctcttcctt ggcttcatcc gcgcccgcca
     1441 cgtccttgaa cgtgatcttg ggattcgatt cgaggaccag cttggcgcgg ctcttgccga
     1501 acttgagtgc ggtggagccg cccgactgca tctggcggat gaagaagagc cagaaggcca
     1561 ggatcaggat gaccgggaga taggtgagca cgaactggag ccagttcgtc tcctcccggt
     1621 cgcccacgat gtcggcggcc gggttcagcg actggatctt ttcggtgagc tccttgctgt
     1681 tctcgaacgg caggatcacc tggaagaaga tgacggaagc ggggtgatcg ccgaccggga
     1741 gcatctcggc cttgccgagc tcgccgtggg catggtcctt ccggaaggcg atcttcttga
     1801 tgttcccctt gtccacctgc tccatgaacg tggtgtaggt gatggcctgt tcctggggct
     1861 tcgtcagccg gtagtagtgc acggccagga cgatgaagag cgtcatcatg atccagaagg
     1921 ccagcgtgcg cagcggccgg ggggagagca tcgcgggccc cttgcgcggg ccaccgccac
     1981 ctcccgagcc tcgaccgttc cggccggagc cgccacggtc gtccgagcgt tcgtgccggc
     2041 gcgggatcag gtctttcgtt tcatgatcgt tcaacgttcg gtccttcttt ccgatgaagc
     2101 cactacccgc catgggacaa ggagttggat cggcaggact cgggcgacga ttcagtttcg
     2161 ggaatgcgag gtggccggtg acccgaccgg gacgccggac ttaccgccat ccagcgtgaa
     2221 agttgcgccc ctgcagccaa gtctcctcat tgtagcgaga gccgggccgg gaatccattc
     2281 ggggcgccgg atgattgcaa tcagttgtcg ttcaacgcct tgcggcccgc gcccggaagg
     2341 ggcccgaacc actcggcgag tagaagatgg tgggtggcct cggtaatcgt tgccgccgcc
     2401 gaccgcgcca cccccacgag ccagagcgct tcatccgttg gtccatctcc cgggggtaac
     2461 ggttcggcca ggaccggcac tccccgccgg gcacccaggg gaacgcgggc gtcgatcagg
     2521 atgtcgctga ccttgcggtg ctgtccggcg ccaaagaggc tcatccgttc tcccgtccgg
     2581 atgttccgga ggaggagacg cggtggaagg ccgtccgcgt tgaagaaggc ggcgcgcggt
     2641 cccggatcga gggaaagggc gcgggctccg ccgctttcga gggaaacgcg gctgatccgc
     2701 agacatcctt cgccccaggg gacctcggtt cccgccacga gcggcacttc caccggttcg
     2761 ggcgtggcgg gctcgatctg ccccgcgggc cgcgggcgtt cgagcgtgag ctcgtcgccc
     2821 gacttcacga ggatcatccc ggaggggaag acccatcgtg cttcgccccg ctggatgagg
     2881 ccgcccatca ccccttcgag gtgcacctgc ccccaggaca ggcgttcggg cgcgatccgc
     2941 tcgccggccg cccggagcac ggctcgcacg acggccggtg gttcggccag gagcgccgtc
     3001 cggtccagga tcacgcgtcc cccgtccacg tccacgcggg cgtgcccgtc gaggtgggcc
     3061 gccgagagtc gcttcagcag ctggtcgatc tcctgctgga gggaagccgt ccgcaccagc
     3121 gccttcaccg cctgcggatt gaagtgctcc ttgaggatcg gcatcagctc gcgacgcacg
     3181 cggttccggg tgaagaccgg gtcgtcgttc gaggcatcct cgcgccaggt gaattcgcgg
     3241 gcggcgagcc actcgcgcag ggccgctcgt tcgagcccga ggagcgggcg caggaagggg
     3301 cgcggtccga ccg
//
LOCUS       DASVOD010000605         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_664232, whole genome shotgun sequence.
ACCESSION   DASVOD010000605 DASVOD010000000
VERSION     DASVOD010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664232"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3628
                     /locus_tag="VF720_13270"
     CDS             <1..>3628
                     /locus_tag="VF720_13270"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX7880379.1"
                     /translation="KDGKPVMAAPPRDRALIRVTDLGLTGKTSVANHLFWLTSLSTGR
                     PVAGASVEVRSASGRLVWSGTTGSDGLVTGNGLPLVEGEEWPLHAIATTADDQVWVTL
                     GGDWNLEPWSFGLSGGSWNPNVDLDGMIYGDRDLYRPGETAHLKVIVRRRSLAGLESP
                     GALAVRVEVDDPRGNPVVRQNATLSRFGTTHLDVPLTAAATPGTWSVRLVPPVTSPTV
                     NWSVYGSFAVAEYKAPEFRVDLSSAKPWTLSGQVLPIDIGASYFFGAPLGGATARWSL
                     SRRPFEFHPAGYDDYVFSDADVTPEYEWLAGRGEATLDKEGKGTLTPVADLGTTPVSL
                     VYTAEVEVRDQTGRASAATLERPVHRGLAYPGIRVSETLVDAGASVSADIVLVAPDGA
                     PAAGASGQVRLVRREWRTVRRLMVGGIVGSETTEADTLLERRTFPSGPGPHRMSFQVP
                     TAGYYRLVVDATDGTKNRQQSAATFYASGPGDYAWGWEPGVQLELVADRKSYRPGDVA
                     RILVRSPFGGAAALVTLEREGLLRHETRRLDGTSEIVEVPIPAEGALPNLYVSVALVL
                     GSQARKGADPRLIKPEFRLGMINLPVDHSGQALRVNLRPERTMVGPRDSVSIALELRD
                     AAGRPVSGEMSVAVVDEAVLRLLGTQTPDPLAWFYRPRPLSVTTSEIRRQVVKTLSYL
                     KAKGGDPGGGGGGGAMFRSLFATTALWTADVVTDANGRARVGVRLPDNLTTFRVMAVA
                     VDEAARFGAADTTILVTKPLLVQAALPRFATTGDQFRGGAVVHNRTDRAALVTVNATA
                     DGLVLKGLPRPAAQSIPAGGSQRFEFDLAATRPGMARVRFSATGSGFSDGIETSFPVL
                     DPVIARYDAATGAVESPVDEVMALPPTARPDSARVEVTLAPTILGGLEPSFRYLDDYP
                     YSCVEQQASRAWARILEARLLDRGSPAVAAAEAVRTLAPYQVEDGGFVLYPGGSASQV
                     GLTAWALLALDESRRAGGNTNALAGPAVRYLGWQLQAQSSDTSLVVMRAADWALALWA
                     MSRWPREEPADAAFRQALTFLTEVRARLSADSRLSLALAWQGSGRDPAGQAGRLLAEL
                     ENHVEQVADMAWLEDGRLHRPIECWEWWWRSPVRTNALYLSALLGREARHPFERKVTK
                     KLLEERTSGRWVNTQANILSLAALAAYRDRYETDTPDLTAQLTLGGAAATAVAFHART
                     DPPVM"
BASE COUNT          511 a         1336 c         1237 g          544 t
ORIGIN      
        1 gaaggacggc aagccggtga tggccgcgcc gccgcgcgac cgggcgctga tccgcgtcac
       61 cgacctcggc ctcaccggca agacatcggt ggcgaatcac ctcttctggc tcacctcgct
      121 gtcgaccggg cgtccggtcg cgggcgcctc ggtcgaagtt cgctcggcgt cgggccgcct
      181 cgtctggtcc ggcacgaccg ggtccgatgg tctcgtcacg gggaacgggc tcccgctcgt
      241 ggaaggggag gagtggccgc tccacgccat cgccaccacg gccgacgacc aggtgtgggt
      301 cacgctcggg ggtgactgga acctcgagcc ctggagcttt ggcctctccg gcggcagctg
      361 gaacccgaac gtcgatctcg acgggatgat ctacggcgat cgcgacctct accgccccgg
      421 cgaaacggcg cacctgaaag tcatcgtgcg ccgccgctcg ctggccggcc tcgagagccc
      481 gggtgcgctg gcggtccgcg tggaagtgga cgatccgcgc ggcaatccgg tcgtccgaca
      541 aaatgccacg ctctcgcgtt tcggcacgac gcacctcgac gtgccgctca ccgcggccgc
      601 cacgcccggc acctggagcg tgcggctcgt cccgccggtc acgagcccga cggtcaactg
      661 gagcgtgtac ggctcctttg ccgtggccga gtacaaggcg cccgagttcc gtgtcgacct
      721 gtcttccgcg aagccctgga ccttgagcgg ccaggtcctg ccgatcgaca tcggcgcgtc
      781 gtacttcttc ggcgccccgc tcggcggcgc caccgcgcgc tggtcgctga gccggcggcc
      841 gttcgaattc cacccggccg gttacgacga ctacgtcttc tccgacgccg acgtgacgcc
      901 cgagtacgaa tggctggccg gtcgcggcga ggccacgctc gacaaggagg ggaaggggac
      961 gctcacgccg gtggccgacc tgggcaccac accggtgagc ctcgtctaca ccgccgaggt
     1021 ggaggtgcgc gaccagaccg gccgggcctc cgccgccacg ctcgaacgac cggtgcaccg
     1081 cggacttgcg tatcccggca ttcgcgtgtc cgaaacgctg gtcgacgcgg gcgcttcggt
     1141 gtcggccgac atcgtcctgg tggcgccgga cggagctccc gcggccggcg cctccggcca
     1201 ggtgcgcctc gtccggcggg agtggcgcac ggtgcgccgc ctcatggtcg gcggcatcgt
     1261 cggatccgaa accaccgaag ccgacacgct cctcgaacgg cgcactttcc cttcgggacc
     1321 ggggccgcac cggatgtcgt tccaggtgcc gacggccggc tactaccgcc tggtcgtcga
     1381 cgccaccgac ggcaccaaga accgccagca gagcgccgcc accttctacg cctccggccc
     1441 cggtgactat gcgtggggat gggagccggg cgtgcagctg gagctcgtgg ccgatcgcaa
     1501 gagctatcgc ccgggcgatg tggcgcgcat tctcgttcgc tcaccgttcg ggggggccgc
     1561 ggcgctcgtc acgctcgagc gcgaaggtct cctccgccac gaaacgcgcc gcctcgatgg
     1621 cacctcggaa atcgtggaag tgccgatccc ggccgaggga gcgctaccca acctctacgt
     1681 gtcggtggcg ctggtcctcg gcagtcaggc gcgcaagggg gccgacccgc gtctcatcaa
     1741 gccggagttc cgcctcggca tgatcaactt gccggtcgat cattcgggcc aggcgcttcg
     1801 cgtgaacctg cgtcccgaac ggaccatggt gggcccgcgc gattcggtgt cgatcgcact
     1861 cgaactgaga gacgccgccg ggcgccccgt ctccggcgag atgagcgtgg cggtcgtcga
     1921 tgaagccgtg cttcgcctcc tcggcacgca gacgcccgac cccctggcct ggttctatcg
     1981 cccgcgcccg ctgtcggtca ccaccagcga aatccggcgg caggtcgtga agacgctgtc
     2041 gtacctgaag gccaaggggg gcgacccggg cggcggcggc ggcggcggcg ccatgttccg
     2101 atccctcttc gccaccaccg cgctctggac ggccgatgtc gtcaccgacg cgaacggccg
     2161 cgcgcgcgtg ggggtgcgcc tccccgacaa cctcaccacg ttccgcgtga tggccgtggc
     2221 ggtcgacgag gccgcgcgct tcggtgcggc cgacacgacg atcctcgtca ccaagcccct
     2281 cctcgtgcag gcggcgcttc cccgcttcgc caccacgggt gaccagttcc gcggcggcgc
     2341 ggtagtgcac aaccgcaccg acagggcggc gttggtgact gtcaacgcca ccgccgatgg
     2401 cctcgtactc aaggggttgc cccgtccggc cgcgcagtcg ataccggccg gaggaagtca
     2461 gcgattcgaa ttcgacctcg cggccacgcg gcccggcatg gcgcgcgtgc gtttctcggc
     2521 aaccggcagc ggcttctccg acggcatcga gacatcattt ccggtcctgg atccggtgat
     2581 cgcccgttac gacgccgcca ccggcgccgt ggagtcgccc gtcgacgaag tcatggccct
     2641 gcccccgacc gcccgccccg actcggcccg cgtggaggtg acgctggcac ccacgatcct
     2701 gggaggactc gaaccctcct ttcgatacct ggacgactac ccgtactcct gcgtcgagca
     2761 gcaggcctcg cgcgcctggg cccgcatcct cgaggcacgc cttctcgatc gcggctcgcc
     2821 cgccgtggcc gcggctgagg ccgtgcgcac tctagccccg taccaggtgg aggacggtgg
     2881 tttcgtcctg tacccgggcg gcagcgcgag ccaggtgggc ctcaccgcct gggcgctcct
     2941 ggcgctggat gaatcgcgac gggccggcgg caacaccaat gcgctggccg ggccggccgt
     3001 ccgctacctc gggtggcaac tccaggcgca gagttccgac acgtcgctcg tcgtcatgcg
     3061 cgcggccgac tgggccctgg ccctctgggc catgagtcgc tggccgcgcg aggaaccggc
     3121 cgatgcggcc ttccgccagg cgctcacgtt cctcaccgaa gtgcgcgcgc gcctctcggc
     3181 cgattcgcga ctttccctcg cgctcgcctg gcaggggagc gggcgcgatc cggctgggca
     3241 ggccggtcgc ctcctcgccg aactcgaaaa ccacgtcgag caggtggcgg acatggcctg
     3301 gctcgaggat ggccggctcc atcgcccgat cgaatgctgg gaatggtggt ggcgctcacc
     3361 ggtccgcacg aacgcgcttt acctttcggc gctgctcggc cgggaagcgc ggcacccgtt
     3421 cgaacggaag gtcacgaaga agctcctcga ggagcggacc agcgggcgat gggtcaacac
     3481 gcaggccaac atcctctcgc tggccgcact cgcggcctac cgcgatcgtt acgagactga
     3541 cacgccggat ctcaccgcac aactcacgct gggcggcgcg gccgcgacgg cggtggcttt
     3601 tcacgcgcga accgacccgc cggtcatg
//
LOCUS       DASVOD010000606         2339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_300592, whole genome shotgun sequence.
ACCESSION   DASVOD010000606 DASVOD010000000
VERSION     DASVOD010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300592"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1087)
                     /gene="rsgA"
                     /locus_tag="VF720_13275"
     CDS             complement(146..1087)
                     /gene="rsgA"
                     /locus_tag="VF720_13275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEX7880380.1"
                     /translation="MLLSGRVVGARSRSFTVETNDGSLTCRVLKGLRNQFPDLVDPVA
                     VGDRVRVRRGNEGDATIEEVEPRVNKISRPAVGQEDREQLIAANLGRAVIVQAVEPRW
                     KTATWDRYLVMAAAGGVPPLLALNKIDLDPASAAADEFDVYRRLGIGVVAVSARTGKG
                     LDELTERLAGGMSVFIGPSGVGKSSLINALGADLTLRTGELSRTTGKGRHTTTWTELL
                     RLDRGIEVVDSPGLRVLGLWGIEPEQLAGTFPEFASRAAGCRFRQCTHAHEPDCAVRD
                     AVADGEIAPFRYDSFLRIREALLEERERPRGGRARRR"
     gene            987..2015
                     /locus_tag="VF720_13280"
     CDS             987..2015
                     /locus_tag="VF720_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880381.1"
                     /translation="MRNPFNTRHVKDPSFVSTVKLRLLAPTTRPERSMMTPLYRYRTQ
                     HVVWFAAAWLVLAGGRAAAQGETQVLPPGAGQATVEGDSTRPRLAIKEITVSGERVDL
                     REILRKAVEGEKKKHEGLTTFSYTRRIRSVMRFEGKKARTRIDDIVQRVYYRAPDQWV
                     EVTAYDSTRTLNPDGTPRKNDDEDDEEKSGLNVSAESRSIEENPFYLERLDRFRFYQG
                     PIHRTDSTAVFEIEFEPVSDFSDDTPGGTLWLLAPEYQIVREEFRFTKGLPASYILKG
                     LDLLTREWQQVEDRWLERRLTGRVTLAQGLGLKQMGVPKTIEFVVEYDDYRMNPELPD
                     AIFKEKGD"
     gene            2015..>2339
                     /locus_tag="VF720_13285"
     CDS             2015..>2339
                     /locus_tag="VF720_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880382.1"
                     /translation="MRRIPTCLAALASILVFMGPARAQDDKAPAEDVNSVLARESARL
                     DSLLSPEARVLDQPGLEDRIRALDAGSFDSLGAAIRRRAGLRISVEPGRTSTFNRAEG
                     FRVATG"
BASE COUNT          406 a          797 c          769 g          367 t
ORIGIN      
        1 gtgccgaggc gctcgattgg ggacgccgta gccggcgcct ctaccgcctt ccggacagca
       61 tggcggcgag cctcccgatt ccgggccgga ccgaagtagc cgcatcattc ggcaaggcca
      121 acttccagta accccctgct gccggttacc gccggcgagc ccgccctccc cttggccgtt
      181 cgcgctcctc gagcagcgct tcccggatcc tcaggaaact gtcgtagcga aacggggcga
      241 tctccccgtc ggccaccgca tccctcaccg cgcagtccgg ctcgtgcgcg tgcgtgcatt
      301 gcctgaatcg acacccggcg gcccgggacg cgaactcggg gaaggttcca gccagctgct
      361 ccggctcaat tccccatagg ccgaggactc gcaggccggg actgtcgacc acctcgattc
      421 cccggtccag gcgaaggagc tcggtccagg tggtggtatg ccgtcccttc ccggtggtgc
      481 ggctcaactc cccggtccgc agggtcaggt cggcgccgag cgcattgatg aggctcgact
      541 tgccgacacc cgacgggccg atgaagaccg acattcctcc cgccagccgt tcggtcagtt
      601 catcgagccc cttgcccgtg cgggccgata ccgcaaccac gccgatgccg agacgacggt
      661 agacatcgaa ttcatccgcg gcggcggaag ccggatcgag atcgatcttg ttgagtgcca
      721 ggaggggtgg cacgccgccc gccgcggcca tgacgaggta gcgatcccag gtggcggtct
      781 tccagcgagg ctcgacggcc tggacgatga cggcccgacc gaggttggcc gcgatcaact
      841 gctcccgatc ctcctgtccc accgccggtc gggagatctt gttgaccctc ggttccactt
      901 cttctatggt cgcgtcaccc tcattaccac gccggaccct gacacggtct cccacggcca
      961 ccggatcgac gaggtccgga aattgattgc gcaacccctt caacacccgg cacgtcaagg
     1021 acccgtcgtt cgtttccacc gtgaagcttc ggcttctggc gccgacgacc cgaccggaga
     1081 ggagcatgat gacgccacta tataggtacc gaacgcagca cgtcgtatgg ttcgcggccg
     1141 cctggctggt gctggcgggc ggacgggcag ccgcccaggg ggagacccag gtgcttccgc
     1201 cgggcgcggg gcaggccacc gtggaggggg actccacccg gccccgactc gcgatcaagg
     1261 aaatcacggt ttccggggaa cgggtcgacc tgcgtgaaat cctgcggaag gccgtggaag
     1321 gggaaaagaa gaagcacgag gggctcacga ccttttccta cacgcgccgc atccggagcg
     1381 tcatgcgctt cgagggaaag aaggccagga ccaggatcga cgacatcgtt cagcgcgtgt
     1441 actaccgggc cccggaccag tgggtggaag tgaccgccta cgacagcacc cggacactga
     1501 acccggacgg cacaccgcgg aagaatgacg acgaggacga cgaggagaaa tccggcctga
     1561 acgtgagcgc cgagagccgg agcatcgagg agaatccctt ctacctcgag cgtctcgatc
     1621 gtttccgctt ctaccagggg ccgatccacc ggacggattc gacggccgtc ttcgaaatcg
     1681 agttcgagcc ggtctcggac ttctcggacg acacgcccgg cggcaccctg tggctcctgg
     1741 cgcccgaata ccagatcgtc cgcgaggagt tccgcttcac gaaggggctt ccggcgagct
     1801 acatcctcaa ggggctcgac ctcctcaccc gcgagtggca gcaggtggag gatcgctggc
     1861 tggagcgccg gttgacggga cgcgtgacgc tggcccaggg gcttggactg aagcagatgg
     1921 gcgttccgaa gaccatcgag ttcgtcgtgg agtacgacga ttaccggatg aacccggagc
     1981 tgccggacgc cattttcaag gagaaggggg attgatgcgc cggatcccga cctgcctggc
     2041 cgcgctcgcg tcgatcctcg tcttcatggg accggcgcgg gcccaggatg acaaggcgcc
     2101 cgcggaggac gtgaacagcg tcctggcccg cgagagcgcc agactcgatt cgctcctgtc
     2161 ccccgaggcg cgggtgctgg accagcccgg gctggaggat cgtatccggg ccctggacgc
     2221 cggatccttc gattcgctcg gcgccgccat ccggcgccgg gcgggccttc gcatctccgt
     2281 cgagccgggc cgcacctcca ccttcaaccg ggcggaaggc ttccgggtgg ccaccggga
//
LOCUS       DASVOD010000607         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_612549, whole genome shotgun sequence.
ACCESSION   DASVOD010000607 DASVOD010000000
VERSION     DASVOD010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612549"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2540
                     /gene="alaS"
                     /locus_tag="VF720_13290"
     CDS             <1..2540
                     /gene="alaS"
                     /locus_tag="VF720_13290"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX7880383.1"
                     /translation="SIVPQDDPTLLFTSAGMVQFKQLYAAEGRLEYTRATSVQKCFRV
                     TDLEQVGHTPRHCTFFEMLGNFSFGDYFKREAIAWAWEFCTVDLKMDPERLWASVYLD
                     DDEAETIWKSEIGIPAERIVRLGRKDNFWGPAGATGACGPCSEIYYDLGPAFGPDTAR
                     PGDEHDRYQEFWNLVFPQFFQDEKGNMGPLAKRGIDTGMGLERLTQILNGQASVFETD
                     AFRPIIEVAADMASVDPDSDAARIPLRVIAEHARALTVATSEGIYPGNEGRGYVIRRL
                     LRRAARRGQVLGLHQPFLYKLSGVVVDLLGDHYTELRTTRERVATVLKAEEERFAATV
                     QQGMHRFEEIAKRVQAQGGTSVSGEDSFLLYDTFGFPFDLTQEMAAERGLTVDQPEFE
                     KEMGRQVERSRAASKFETAAGASDRAWEWVAPATEATKHSEFTGYECLADEVRLVAHR
                     SAGDGIEFLLDKTPFYAESGGQVADTGRLESPGGRIRIDSVKKNDKGEIVHRGTLLDG
                     ALSAGPWQAAVPGETRRATERNHTATHLLHAALREVLGTHVAQAGSLVAPDRLRFDFA
                     HFASITPGQRDEIERLVNAAILADIPVETQWGSLADARAAGAMALFGEKYGDRVRQVI
                     VSDVSRELCGGTHVRRTGEIGAFRIIAEESVASGTRRVEAATGWKALRLLADDEALID
                     RLAERLKAGRGDVEEKLGHLLEENQKLRHELDGVKGKALAAKTGELGASIVEFKGIRF
                     LAAELPADSLDRLRDAADALKPQLRSGAAVLGARFDGKAMIVAIVTDDLVAAGRLKAD
                     QVVREVAKIAGGSGGGKPTFATAGAKQPEKLGDAIGQAAGIVETLLG"
     gene            complement(2579..3196)
                     /locus_tag="VF720_13295"
     CDS             complement(2579..3196)
                     /locus_tag="VF720_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880384.1"
                     /translation="MGRDVTCPVTVNGETRTARVLLETGEIIIQGRPRLKVAFRDMKE
                     LAAGGGILSFRGEVGQVRIDLGAKAAEWLASIRTPTPRLDKLGVKLGMTVIATGLDDD
                     LFLAEVTARIGAPVRGRLSGRADIVFLGARSATDLGRLAAARRAIAPDGTIWVVWPKG
                     RKEFGENHVRASALDTGLVDVKVVAFDDTMSALKLVIPLKLRGRA"
     gene            3211..4131
                     /locus_tag="VF720_13300"
     CDS             3211..4131
                     /locus_tag="VF720_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880385.1"
                     /translation="MPVVVADHPAHRAVALWAMILLALAAPGCGGEDDDELSHDEREV
                     RNAGITWWRQLDVALIANTAALDYGAGGLTPPSSRPDPGPFGAVPSGPVRGALQPAHA
                     YGINCPDVTPAVTGDLMRVYVVYGDLGCQSDLSGQRQTGYVQLDVTLPTKTGTLVAAN
                     LATGDYRVNGTIGATYQDTMVTIDARSLMVSSPEGSVTITSCLGIFWSMGGSRELLSD
                     DSWRILGTSTVNTLSLESGAIYHLEVPEDDPILANPRCAWPVSGRVDIQRQEPPLAAT
                     LDFGDGACDSEAILTVAGRTRIVDLENVSD"
     gene            4237..>4488
                     /locus_tag="VF720_13305"
     CDS             4237..>4488
                     /locus_tag="VF720_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7880386.1"
                     /translation="MKALILSGGRGTRLRPITHTSAKQLVPVANKPILFYGIEAIRNS
                     GITDIGIVVGDTREEIKAAVGDGSAFGVRVTYIHQQDPLG"
BASE COUNT          726 a         1572 c         1514 g          676 t
ORIGIN      
        1 cttccatcgt cccgcaggac gacccgacgc tcctcttcac ctcggcgggc atggtgcagt
       61 tcaagcagct ctacgcggcc gagggacggc tcgagtacac gcgcgccacc tcggtgcaga
      121 agtgcttccg cgtgaccgac ctggagcagg tggggcatac accgcgccac tgcaccttct
      181 tcgagatgct gggcaacttc agcttcggcg attacttcaa gcgcgaggcc atcgcctggg
      241 cgtgggagtt ctgcacggtg gacctcaaga tggaccccga gcggctgtgg gcctcggtat
      301 acctggacga tgacgaagcc gagaccatct ggaagagcga gatcggcatt ccggccgaac
      361 ggatcgtgcg cctcggcagg aaagacaact tctggggtcc ggccggcgct accggcgcct
      421 gcggcccctg cagcgagatc tactacgacc tgggtcccgc cttcggccct gataccgccc
      481 gccccgggga cgagcacgac cgttaccagg agttctggaa cctcgtcttc ccgcagttct
      541 tccaggacga gaaggggaac atgggtcccc tggccaagcg cggcatcgac actggcatgg
      601 gactggagcg cctcacgcag atcctgaacg gccaggcctc ggtcttcgag accgacgcct
      661 tccggcccat catcgaggtg gccgccgata tggcctcggt cgatcccgat tccgacgccg
      721 cgcgcatccc gctccgggtc atcgccgagc atgcccgcgc gctcacggtg gccaccagcg
      781 aagggatcta cccggggaac gaggggcgcg gctacgtcat ccgccgcctg ttgcgtcggg
      841 ccgcccgtcg cggccaggtg ctcggcctcc accagccgtt cctctacaag ctgtcgggcg
      901 tggtggtcga cctcctcggt gatcattaca ccgaactccg caccacccgc gagcgcgtgg
      961 ccacggtgct caaagccgag gaagagcgct ttgccgccac cgtccagcag gggatgcatc
     1021 gcttcgagga aatcgcgaag cgcgtgcagg cccagggcgg cacttcggta tcgggcgagg
     1081 actcgttcct cctgtacgac accttcggct tcccgttcga tcttacgcag gagatggcgg
     1141 ccgagcgcgg cctcaccgtc gaccagccgg aattcgagaa ggagatgggg cgccaggtcg
     1201 agcgaagccg ggccgccagc aagttcgaga cggccgccgg ggccagcgac cgcgcgtggg
     1261 agtgggtggc ccccgccacc gaggccacga aacacagcga gttcaccgga tacgaatgcc
     1321 tggccgacga ggtccgcctc gtagcccacc gttcggccgg cgacggcatc gagttcctgc
     1381 tcgacaagac ccccttctac gccgagagcg gcggccaggt ggcggacacc ggtcgcctcg
     1441 aatcgcccgg cggtcgcatc cgcatcgatt cggtcaagaa gaacgacaag ggggagatcg
     1501 ttcatcgcgg cacgctgctc gacggcgcgc tgtcggccgg tccgtggcag gccgccgtgc
     1561 cgggcgaaac gcggcgggcc accgaacgga atcacaccgc cacgcacctc ctgcacgccg
     1621 cgctgcgcga ggtgctcggc acgcacgtgg cccaggcggg ttcactcgtg gcgcccgacc
     1681 gcctccgttt cgactttgcc cacttcgcgt cgatcacgcc ggggcagcgg gatgagatcg
     1741 aacgtctcgt gaacgccgcg atcctggccg acattccggt cgagacgcag tggggctctc
     1801 tggccgatgc ccgcgcggcc ggcgccatgg cgctgttcgg cgaaaagtac ggcgaccgcg
     1861 tccggcaggt gatcgtctcc gacgtcagcc gcgagctgtg cggcggcacg cacgtgcgca
     1921 ggacggggga gatcggggcg ttccgcatca tcgccgagga atcggtggcc tcgggtacgc
     1981 ggcgcgtgga ggccgccacc ggctggaagg cgctgcgcct cctggccgat gacgaggcgc
     2041 tgatcgatcg cctggccgaa cgcctgaagg ccggccgtgg cgacgtggag gagaagctcg
     2101 gccaccttct cgaggaaaac cagaagctgc gccacgaact ggacggggtg aagggaaagg
     2161 ccctggctgc caagaccggc gaactgggcg cctcgatcgt ggagttcaag gggatccgct
     2221 tcctggccgc tgaactgccg gccgacagcc tcgaccggct gcgcgacgcg gccgatgccc
     2281 tcaagcccca gctcaggagc ggcgccgccg ttctcggtgc ccgcttcgac gggaaggcca
     2341 tgatcgtggc catcgtcacc gacgacctgg tggccgcggg tcggctcaag gccgaccagg
     2401 tggtgcgcga ggtggcaaag atcgccggcg gttcgggggg cggaaagccc accttcgcca
     2461 ccgccggcgc caagcagccg gaaaaactgg gcgacgccat cgggcaagcg gccgggatcg
     2521 tcgagacgct gctggggtga gcggggcgtg gcgcggcttc gcgctgcccc gcttcgcgct
     2581 aggccctgcc gcgcagtttc agcggaatca caagtttcaa cgccgacatc gtgtcatcga
     2641 acgcgaccac tttcacgtcc acgagcccgg tgtcgagcgc cgatgccctg acatggttct
     2701 cgccgaattc cttccgcccc ttcggccaca ccacccagat cgtgccgtcg ggcgcgatgg
     2761 ccctgcgcgc cgcggccagt cggccgaggt cggtggccga ccgtgccccg aggaagacga
     2821 tgtcggcccg tccgctgagg cgcccgcgaa cgggcgcgcc gatccgtgcc gtaacttcgg
     2881 ccaggaagag gtcgtcgtcg aggcccgtgg cgatcacggt cattccgagt ttcacgccga
     2941 gcttgtcgag tcgcggcgtc ggggtgcgga tcgaggccag ccactcggcg gccttcgcgc
     3001 cgaggtcgat gcgcacctgt ccgacctccc cccggaacga gaggatgccg ccgccggccg
     3061 cgagctcctt catgtcacgg aaggccacct tgagtcgggg ccgcccctgg atgatgatct
     3121 ctccggtctc gaggagcacc cgggccgtcc gggtctctcc gttcaccgtc accgggcagg
     3181 tgacatcgcg ccccatcgag gaatccccgc gtgcccgttg tcgtcgccga tcatcccgcg
     3241 caccgcgccg tcgccctctg ggcgatgatc ctcctcgccc tggccgcccc gggctgcggc
     3301 ggcgaggacg acgacgaatt gtcgcacgac gaacgggaag tgcggaatgc gggcatcacc
     3361 tggtggcggc agctcgacgt cgcgttgatc gccaacaccg cggcgctcga ttacggtgcg
     3421 ggagggctca cccctccctc gagccgcccg gaccccggcc ccttcggcgc cgttccgtcc
     3481 ggcccggtgc gcggggcgct ccagccggcc cacgcctacg gcatcaactg tcccgacgtg
     3541 acgccggccg tcacgggcga cctcatgcgc gtctacgtcg tgtacggaga cctcgggtgc
     3601 cagtcggatc tctccggcca gcggcagacc ggctacgtgc agctcgacgt gacgctcccg
     3661 acgaagaccg ggacgctggt ggcggccaac ctggctaccg gcgattaccg ggtgaacggc
     3721 acgatcggcg ccacctacca ggatacgatg gtgacgatcg atgcccggtc gctcatggtg
     3781 tcgagcccgg aggggagcgt cacgatcacc tcgtgcctcg ggatattctg gtcgatgggg
     3841 ggttcgcgcg aactcctctc cgacgactcc tggcgaatcc tcggcacctc caccgtcaac
     3901 acgctgagcc tcgaatcggg cgccatctac cacctcgaag tgccggagga cgatccgatc
     3961 ctggccaatc cgcgctgtgc gtggccggtg tccggccgcg tggacatcca gcgccaggaa
     4021 ccgccacttg ccgccacgct cgatttcggc gatggcgcct gcgactcgga ggccatcctg
     4081 accgtggcgg gacgcacgag gatcgtcgac ctcgagaacg tgtcggattg acacaagaca
     4141 ttctcggctg atactttagc gcccttcgtt caagtgcagg gacaaggctg cgggagcggc
     4201 cgtaccaggg cggccatccg cgggaaggac cgagtcatca aagcgctgat cctctcgggc
     4261 gggcgtggca cccgcctccg gcccatcacc cacaccagcg ccaaacagct ggtgccggtg
     4321 gccaacaagc ccatcctctt ctacggcatc gaggccatcc gcaactcggg gatcaccgac
     4381 atcgggatcg tggtggggga cacccgcgag gagatcaagg ccgccgtcgg tgacggttcg
     4441 gccttcgggg tcagggtcac ctacatccac cagcaggacc ccttgggc
//
LOCUS       DASVOD010000608         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_873849, whole genome shotgun sequence.
ACCESSION   DASVOD010000608 DASVOD010000000
VERSION     DASVOD010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873849"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF720_13310"
     CDS             <1..542
                     /locus_tag="VF720_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX7880387.1"
                     /translation="GNHRHVAILKGPPGNLDAQERLLGYRKALREQRTKVTVDYIDGN
                     FSEESGVTAAQKLLKASPRPTAVFAANDTMAIGLIAAFQAGGIRVPEDVAVTGFDDIL
                     ISRYVSPPLTTVRVDACDLGERAVRLLIGPAGREPNDDVRMADAITLPVNLIVRQSCG
                     RASAPTRATAKRATERRSQ"
     gene            539..2470
                     /locus_tag="VF720_13315"
     CDS             539..2470
                     /locus_tag="VF720_13315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7880388.1"
                     /translation="MKRNGYLLMVLGGLWLTAMPAAGAYAPRQDAMWARTSTVPITLD
                     GNLNEASWATAESVLVVYGSSSGIPGSGWKLEAGFTPTNPTNATLKFLVRNNQLYLGA
                     TVRDKSIGGHSTFNRWDGFLMALKDHLSPENPKPPVEYFYSWWYPLSPDPQPVGQKPI
                     FRGRYGVTAPADTTAPDDTTRTPANIAAWDARIVVNGQTNTDATPDVGYTVEMRFDLG
                     VVGYNTTRPEGDIVEWNISIYDCDCFWPLAPNCASGANFSVNRTWFQSPWGNAYWYDE
                     VHLFARPSVTSTSGPVPPIPPEMDIPEIPGAAPVVNGVLNEAIWNNVQSQWDIRYGDN
                     ELRSTYPGVGPWRSGQFQPTVNGGTATVEDGGDATVTALVKGDWLYLGFDVRDQAVQY
                     HADFDRWDGAIVTLTDRTVLGPDNERLTRRISFQVGAAGNAVAQDYLLTLLGSGGAQI
                     GLTLKAGSTVDTTADLAPDTGWQAELAINLTQMGYPSGLGDRALFLGINLLDGDSFSN
                     YTDSYSTRTWWFREYQGQCCPVWAHIIPLSATGVPDESSRPPMPDHLFGAYPNPSGHP
                     RIRYALVAAGKVSVSLFDVSGRLIRTEDLGRIEAGEQLREMNLEGQSSGLYLYRIDVA
                     DPVTGFVRSTLPGRLVYTK"
BASE COUNT          453 a          933 c          779 g          371 t
ORIGIN      
        1 acggcaacca ccggcacgtg gccatcctga aaggaccgcc cggaaacctc gatgcgcagg
       61 aacggctcct cggctaccgc aaggcgctcc gcgagcagcg gacgaaggtg accgtcgact
      121 acatcgacgg caacttcagc gaggagtcgg gcgtgacggc cgcccagaag ctcctgaaag
      181 catccccccg accgacggcc gtgttcgccg ccaacgacac catggccatc ggcctgatcg
      241 cggccttcca ggcgggcggc atccgggtgc cggaagacgt ggccgtcacc ggcttcgacg
      301 acatcctcat ctcccgctac gtaagccctc ccctcaccac cgtgcgcgtg gacgcctgcg
      361 atctcggcga acgggccgta cggctcctca tcgggcccgc cgggcgcgag ccgaacgacg
      421 acgtgcgcat ggccgacgcc atcacccttc ccgtcaacct catcgtgcgg caatcgtgcg
      481 gacgcgcctc ggcgccaacc cgcgccaccg cgaaacgtgc aaccgaaagg cgcagccaat
      541 gaaacggaac ggctaccttc tgatggtcct cggcggactg tggctcacgg ccatgccggc
      601 ggcgggcgcc tacgcgcccc gccaggacgc aatgtgggcc cgcacctcca ccgtgccgat
      661 cacgctcgac ggcaacctga acgaagcctc ctgggccacc gcggaatcgg tcctggtggt
      721 gtatggctcg agcagcggca tccccgggag cggctggaag ctcgaggccg gtttcacccc
      781 gaccaacccc accaacgcca cgctcaagtt tctcgtccgc aacaaccagc tctatctcgg
      841 cgccaccgtg cgcgacaagt cgatcggcgg ccattccacg ttcaaccgct gggacggctt
      901 cctgatggcg ctcaaggacc acctgagccc cgaaaatccg aagccgcccg tcgaatactt
      961 ctactcctgg tggtatcccc tctcccccga cccgcagccg gtgggacaga agccgatctt
     1021 ccgcggccgc tacggcgtga cggccccggc ggacaccacg gcccccgacg acaccacccg
     1081 cacaccggcc aacatcgccg cgtgggacgc ccgcatcgtc gtgaacggcc agacgaacac
     1141 cgatgccacg cccgacgtcg ggtacacggt cgaaatgcgc ttcgacctcg gcgtggtcgg
     1201 ctacaacacc acgcgccccg aaggggacat cgtggaatgg aacatttcca tctacgactg
     1261 cgactgcttc tggcccctgg ccccgaactg cgcgagcggc gccaacttct ccgtcaatcg
     1321 cacctggttc cagagtccgt gggggaacgc ctactggtac gacgaggttc acctcttcgc
     1381 caggccgagc gtcaccagca cctcgggccc ggtcccaccc attccgcccg aaatggacat
     1441 ccccgagatt cccggggcgg ccccggtcgt gaacggcgtg ctgaacgagg ccatctggaa
     1501 caatgtgcag agccagtggg acatccgcta cggcgacaac gaacttcggt caacctaccc
     1561 cggcgtgggt ccgtggcggt ccggccagtt ccagcccacg gtgaatggcg gcacggccac
     1621 ggtggaggac ggcggcgatg ccaccgtcac ggccctcgtg aagggcgact ggctctatct
     1681 cggcttcgac gtccgcgacc aggcggtgca gtaccacgcc gatttcgacc gatgggacgg
     1741 cgccatcgtc acgctgaccg atcggaccgt cctggggccc gacaacgagc ggctcacccg
     1801 gcgcatctcg ttccaggtag gggcagccgg caacgcggtc gcgcaggatt acctcctcac
     1861 actcctcgga agcggcgggg cccagatcgg actgacgctc aaggccggca gcaccgtcga
     1921 taccacggcc gacctcgccc cggacaccgg ctggcaggcc gagctggcca tcaacctgac
     1981 gcagatgggt tacccgtccg gcctcgggga ccgggccctc ttcctcggca tcaatctcct
     2041 ggacggcgac tcgttcagca attacaccga cagctacagc acccgcacct ggtggtttcg
     2101 cgagtaccag ggacagtgct gcccggtgtg ggcccacatc atcccgctgt cggccaccgg
     2161 cgttcccgac gagtcgtcgc gacctccgat gcccgatcac ctcttcggcg cctatcccaa
     2221 tccctccggg catccgcgaa tccggtatgc gctggtggcc gccggaaagg tttccgtctc
     2281 cctgttcgac gtgtcgggtc gcctgatccg caccgaggac ctgggacgga tcgaggccgg
     2341 cgagcagctc cgggaaatga acctcgaggg gcagagcagc ggtctctatc tctatcgcat
     2401 cgacgtggcc gacccggtga cgggtttcgt gcgctccacg cttcccggcc gactggttta
     2461 caccaagtag gcgtcgatgg ccaatccggg cggaggagtc gggacgcagg ggacaagacc
     2521 ggggagccgg cggatc
//
LOCUS       DASVOD010000609         4499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_979828, whole genome shotgun sequence.
ACCESSION   DASVOD010000609 DASVOD010000000
VERSION     DASVOD010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979828"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(248..2080)
                     /locus_tag="VF720_13320"
     CDS             complement(248..2080)
                     /locus_tag="VF720_13320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880389.1"
                     /translation="MITMLLASFGTAQAGRLSHASILLHALPAGGADPCGLQPSGNFV
                     PVTAAPVDPSGADDYYVYVIGHDPDWAVGAGLAGLQFGTQYEADGPGDGLVVHDFEVC
                     ATIDFSTVIPPWPSSGSGTLLTWDPINACQRQPYAVGGYFYLTAYSPSIMAVTTRPLD
                     NLAKVANCASQETVVYPGRLGWISFGGAEYNDDTNGFDPTHDAPMFTRPVLDITAPGE
                     GITLGNNDVSFEWCLIDDETPNDQMNTTWNLDGGDWRTAPSIGRLTVRDLAAGPHVFW
                     MRAVDQDGEVAVDSLAFSVISGLNDFPTTRIIQEPVASPTGPYRFRWTGSDTETPTVN
                     LRYMYFLQEVAYGWCPRPTPQHMYAVSDTEVEFPSLPHGTYVLTVKAIDEHGQVDPIG
                     AKSNLLIVIRDVREYPNCAPWVEITGEPDSLLTEHTLDLHWRGYYGKGPHDQLRFAWR
                     LDGGAWSAWQADSTTSFTFAEPGAHRIQVKARDAFGFETPHLSHVDWNVTDMPAQLRL
                     PTPIMPIPVRGITTEVMITPNPSPKGVSIRFGLGAPMPVGYRVYDVRGRLVRDSGSRE
                     LVPGEQRLDWDGRDAGGRLAPAGVYYIELGVGAERVVRTVSLVR"
     gene            complement(2231..4087)
                     /locus_tag="VF720_13325"
     CDS             complement(2231..4087)
                     /locus_tag="VF720_13325"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880390.1"
                     /translation="MRVGRNRLLHVRSGFALLPFLILLLLPYPALAAPLEHPVILLHI
                     AAPTGKNPCGSGLVGGDGVVTAAPLDSPGPNSYFVYVLAGNAGREGPRSLAGVQFGIE
                     YDGGMGTGIVVDDWRRCTTLEFTSTTIPPLWPAAGSGNLLTWDPLNNCQTAELAVVGY
                     FSVSVYSPATMRIVPRPTDGMAAVAECDPPILTDVYPRRLGLVSFGGASHEGDVDGFN
                     PSREPPAATDPVITFTGRGNWENVADDDVRFEWSVSDDVPHLEGVRQSWRVDNGAWVD
                     VLEGRDFRAPRAENLEEGVHTLQVRATDAENQKTLASATFATTVPRNLPPSPRILTSL
                     PANPTGLALLWTAEDDRTPADLMEYEFDFRQLDLGYLESHADDWRRDTEIVIERLPIG
                     SYQFQLRARDMDGDESTEPEVIRFEVTEGPTGEPPRVSLEGPGFDEEVTDPDVTWRVW
                     GFDDTMERTDLQYSWRFDSGAWSPWDTTTVRSFPITSTGRHRVSVRARDLDGLVTWTP
                     DYREFLFVGTPLALARPAPSVSLAGIHPNPSRGPTQFVFDVMRPGTVTMDVLDVKGRL
                     VQRLLDGYRDAGSLQLAWDGRDREGRLVAAGVYLVRLHSESDESTGRITIIR"
     gene            complement(4091..>4499)
                     /locus_tag="VF720_13330"
     CDS             complement(4091..>4499)
                     /locus_tag="VF720_13330"
                     /inference="COORDINATES: protein motif:HMM:NF013380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEX7880391.1"
                     /translation="TPADAMAMLKETGCDLVMIGRASMKNPWIFQQCAAELAGRPWSE
                     PTALQRRDLILEHFRLLMTGEDEKTAFHKIRTFAGWYTHGLPGGRGLRQRINGIPDSP
                     SFIRAVEEFFEELLAGGASVTPPDSAEPEMAEV"
BASE COUNT          805 a         1426 c         1513 g          755 t
ORIGIN      
        1 taccaaaact ctggaaggcg ctgtgcggca ctcggctact tcatgattcg gtacaatcag
       61 cgccgtccac atggcggact caaaggccaa acgcccttca gcagactgcg gaggtttggt
      121 gaataacgtg tctagaaatc acagctagag agcgttcccg tatctgaggg cgagaacccg
      181 ctatctccgt aaacgatccg cgacggcgtg acatacacgc cgccgcgggt cgcagaatag
      241 ctttacccta ccgcaccaaa ctaaccgtcc gcaccacccg ctccgcgccg acacccagct
      301 cgatgtaata gacaccggcc ggcgcgagcc ggccgcccgc atcccgcccg tcccagtcca
      361 ggcgctgctc cccgggcacg agctcccggg aaccggagtc gcgcaccaat cgcccgcgca
      421 cgtcgtagac gcggtagccg acgggcatgg gggcgccgag gccgaagcgg atcgacacgc
      481 ctttcgggct gggattcggc gtgatcatca cctcggtcgt gatgccacga acgggaatgg
      541 gcatgatcgg cgtcgggagc cggagctgtg cgggcatgtc ggtgacgttc cagtcgacat
      601 ggctcaggtg gggcgtttcg aagccgaagg cgtcgcgggc cttcacctgg atgcggtgcg
      661 caccgggctc ggcaaaggtg aaactggtgg tgctgtcggc ctgccaggcc gaccaggcgc
      721 cgccgtcgag gcgccaggca aagcggagct ggtcgtgcgg tcccttgccg taatagccgc
      781 gccagtggag gtcgagtgtg tgctccgtca ggagcgagtc gggctcgccg gtgatctcca
      841 cccacggggc gcagttggga tactcgcgga catcgcggat cacgatgagg agattgctct
      901 tcgcaccgat cgggtcgacc tggccgtgct cgtcgatggc cttgacggtg agcacgtagg
      961 tgccgtgcgg cagcgacggg aattccactt cggtgtcgga cacggcgtac atgtgctgcg
     1021 gcgtggggcg cggacaccac ccgtaggcaa cttcctgcag gaagtacatg tagcggaggt
     1081 tgacggtcgg cgtttccgta tcggaaccgg tccagcggaa gcggtagggt ccggtcggcg
     1141 acgccacggg ctcctggatg atccgcgtcg tcgggaagtc gttgaggccg gagatgacgc
     1201 tgaacgccag gctgtcgacg gccacctcgc cgtcctggtc cacggcgcgc atccagaaga
     1261 cgtgcggacc ggcggccagg tcacgcacgg tgagccggcc gatcgacggg gcggtgcgcc
     1321 agtcgccgcc gtcgaggttc cacgtcgtgt tcatctgatc gttcggggtt tcatcgtcaa
     1381 tcaggcacca ctcgaagctc acgtcgttgt tcccgagcgt tatcccctca cccggggcgg
     1441 tgatgtcaag caccgggcgc gtgaacatcg gcgcgtcgtg cgtcggatcg aagccgttgg
     1501 tgtcgtcgtt gtattcggcc ccgccgaacg atatccagcc gagccggccg ggatacacca
     1561 cggtttcctg agaggcacag ttggccacct tggccaggtt gtcgagcggc cgcgtcgtca
     1621 ccgccatgat cgacggtgaa taggccgtga ggtagaagta accccccacc gcgtacggct
     1681 ggcgctggca ggcgttgatc ggatcccacg tgagaagcgt gccgctcccc gatgagggcc
     1741 acggcgggat caccgtcgag aagtcgatcg tcgcgcacac ctcgaagtcg tggaccacga
     1801 ggccatcccc cggcccgtcg gcttcgtact gggtgccgaa ctgcagtccg gccaacccgg
     1861 ccccgacggc ccagtccggg tcgtgaccga tgacataaac gtagtagtcg tccgcccccg
     1921 atggatcgac gggcgcggct gtcaccggca cgaagttccc ggatggctgg aggccgcacg
     1981 gatcggcgcc cccggcgggg agggcgtgaa ggaggatcga ggcgtgggag agtcgcccgg
     2041 cctgggcggt gccgaatgac gccagaagca tggtgatcac ggtcaggaag cttcgggcga
     2101 tgacggacga gcaggcaggc atagggcggg gagaggacat ggcggcagat tcctccttgg
     2161 tggcaagccc gctacagtgt aacgaacatt tacatccgcg ccagtggcag ggggccggaa
     2221 tcgtcgcaat ctatcgaata atcgtgatcc tgcccgtgct ttcgtcactc tccgaatgga
     2281 ggcgtaccag gtagacgccc gcggcgacca acctcccctc ccggtcccga ccatcccagg
     2341 ccagctggag cgacccggca tcgcgatagc cgtcgagcag ccgctggacg aggcgcccct
     2401 tcacgtcgag tacgtccatc gtcacggtgc ccggcctcat gacgtcaaag acaaactgtg
     2461 tgggaccgcg cgacggattg ggatggatgc ccgccaggct caccgacgga gccggccggg
     2521 cgagggcaag cggcgtgccg acgaagagga attcacgata gtcgggagtc cacgtgacca
     2581 atccgtccag gtcccgcgcg cgcacgctga cgcggtggcg gccggtggag gtgatgggaa
     2641 acgaccgcac ggtcgtggtg tcccacggac tccaggcccc gctgtcgaaa cgccaggaat
     2701 actggagatc ggtgcgttcc atcgtgtcgt cgaatcccca gacacgccag gtcacgtcgg
     2761 gatcggtcac ttcctcgtcg aatccggggc cttcgaggct tacccggggg ggctcgccag
     2821 ttgggccctc ggtgacctcg aaccggatca cctcaggttc ggtcgactcg tcgccgtcca
     2881 tgtcacgggc acgaagctgg aattgatagc ttccgatcgg aagtctctcg atgacgattt
     2941 ccgtatcgcg ccgccagtca tcggcatgac tttcaaggta tcccaggtcg agttgccgga
     3001 agtcgaattc atactccatg aggtcggccg gcgtccggtc atcctcggcc gtccagagga
     3061 gggccagccc ggtggggttc gctggcagtg acgtcaggat gcgggggctg ggtggcaagt
     3121 ttcgagggac cgtcgtggcg aacgtggccg acgccagcgt cttctggttt tccgcgtccg
     3181 tggcgcgcac ctgcagggtg tggactcctt cctccaggtt ttcggcgcgg ggcgcccgga
     3241 agtctcgccc ttccagcacg tcaacccagg cgccgttgtc tacccgccag gactggcgaa
     3301 caccttccag gtggggcacg tcgtcgctca ccgaccattc gaaccggaca tcatcgtccg
     3361 ccacgttctc ccaatttcct cggccggtga aggtgatgac cggatcggtg gcggcggggg
     3421 gctctcggga ggggttgaaa ccatcgacgt caccttcgtg ggaggcgcca ccaaagctca
     3481 ccaatccgag tcgccgcggg tagacatcgg tcaggatggg aggatcgcat tcggcgacgg
     3541 ctgccattcc atcggtcggg cggggcacga tccgcatcgt tgccggggaa tagacactga
     3601 ccgaaaaata gccgaccacg gccaattcag ccgtctggca gttgttgaga ggatcccagg
     3661 tgaggagatt gccgcttccc gccgccggcc agagcggcgg gatcgtagtc gatgtgaact
     3721 caagggtcgt gcaacgacgc cagtcgtcca ccacgattcc ggtccccatt cccccgtcgt
     3781 attcgatccc gaattgaacg ccggccaagg accgcggacc ctcccgtccc gcattccccg
     3841 ccagcacata tacgaaatac gagttcgggc ccggcgaatc gagcggggcc gccgtgacca
     3901 ccccatcgcc cccgacaagc cccgaaccgc acggattctt tccggtagga gcggcaatgt
     3961 ggagaaggat gaccgggtgt tcgagaggtg cggcaagggc gggatacggg agcaacagga
     4021 ggatcaggaa cggcaggaga gcgaatcccg accgtacgtg cagcaatcga ttgcggccaa
     4081 ctctcatcag ctacacctcc gccatctccg gttcggcact atcgggcggc gtgacactcg
     4141 ctcccccggc caggagttcc tcgaaaaact cctccacggc acgaataaaa ctcgggctgt
     4201 ccggaattcc gttgatccgc tggcgcaggc cgcgcccccc cggcaggccg tgcgtgtacc
     4261 agccggcgaa ggtgcggatc ttgtggaaag cggtcttctc gtcttcgccg gtcatcagga
     4321 ggcggaagtg ttcgaggatc aggtcgcgcc gctgcagcgc cgtgggctca ctccagggac
     4381 gaccggccag ttcggcggcg cactgctgga agatccaggg gttcttcatg gaggcgcggc
     4441 cgatcatgac gaggtcgcag ccggtttcct tgagcatggc catcgcgtcg gcgggggtg
//
LOCUS       DASVOD010000610         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_769130, whole genome shotgun sequence.
ACCESSION   DASVOD010000610 DASVOD010000000
VERSION     DASVOD010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769130"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..2270
                     /locus_tag="VF720_13335"
     CDS             609..2270
                     /locus_tag="VF720_13335"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX7880392.1"
                     /translation="MDSFRWLSDGSGIVFKSLQPLSSEDQKRSDSDRSRGFDGRSSGV
                     SSRAWEFRIWRLEADKSSLITVPGPGVGDWDLSPDDNYIVYASNGWEPHDEESPTDLW
                     IHDRAAGSTRRVTTRWGPETQPVFSRDGEWIAYKADQDSSITYSQEELRVVSVSGLVG
                     HQVSGDLDLALDKPFWATGSRVLSAEVSQGLDNGRVEFSPGPLYLTLPDGSRMRGDIP
                     APIEAMLQATVGRYDSEFSAAQNASVITWIGESSGEWPEVWLSYEVGRSGKISREGQW
                     PDPAPVSSLESGTFGKGVGDFRRIRSTAPDGTVTESLLALPDTTLYGPGPYPTITQLH
                     GGPYNRNTARPRDNPTALVLASHGYLVYMPNFRGSKGYGNAFGTAARGRDFAEGPYRD
                     VMAGLDSLIARGLADSTRLGIMGGSYGGYLSMFAISRTDRFKAAVSMYGMSSLITDFG
                     NGKSPSYELEYLGEPYWKGNPLWTELSPAWHVDKIHTPLLLLHGENDQSVGISNSREM
                     WTALTVLKRPVEFITYPREGHGFREPNHRIDACRRTVAWFDRYLK"
     gene            2228..>2995
                     /locus_tag="VF720_13340"
     CDS             2228..>2995
                     /locus_tag="VF720_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880393.1"
                     /translation="MSADGGLVRPVFEIGRGARLFRFQAAVALLLAGTAVAPVDRAVA
                     APTNPDSLSAEAATPVTVLYQGRLTDGGGTAIPGPVTLQVALYSSASAGTPLWTETHP
                     NVPLTPEGIYSILLGSLTPFPANAWTDPDRWLGTTVNGGDELQPRMRVASVPFAIEAN
                     RLNGKRSTDFDPIGAADIAAAGVSAQLKGNDATPPNQGSNQVHWNNLYGMPDGFTDGT
                     DDLGAGVTDHGALTGLADNDHPQYLLGAAAATSDGNAP"
BASE COUNT          530 a         1001 c          955 g          509 t
ORIGIN      
        1 gttgatgacg acaggtccgg tcgagcacac acgctgcccc aacaggttcc ggaacctgtt
       61 ccgtgatggt ctttcgacat caggtaactg tggttagccg cagttggcaa tggagccgac
      121 tccattgcaa gaacctcatc gtggcagccg ccctccttgt cgcgtgcgtc gccgcaacct
      181 gcattgcacg cggggctgcc accgccgcgc ccactcagcg gatcacacgt ccgctcgaca
      241 tggccgacct cgtgacccgg ccggtgccgt cggaaccatg tatgtcaccc gatggcacgg
      301 aggtggcgta cgtcgtgagc cagtcggaca ccatcctgaa tctgcgaacc ggggacattt
      361 ggagtgtgcg agtggaagcg ggcgacaagt cgcccggcat tccgcttcga tggtcacaaa
      421 cgcctgatcc ggagacggag ttgcgatggt ccccgatggg tcatcacctg gcgtttcgag
      481 cccgatccgg cggaggccca ggggaggcga aaagctcagc cgcccggccc gcatccggcc
      541 cctggcaggt gtggatccag tcctcttccg actccgggcg acgacaactc acgagggcac
      601 cggagggcgt ggacagcttt cgatggttga gtgacggttc gggaatcgtc ttcaagtcac
      661 tccagccatt gtcatcggaa gatcagaaac gcagcgactc cgacagaagc cgcggttttg
      721 atggccgttc gtccggcgtt tcctcgcgtg cctgggagtt ccgcatctgg cgcctcgagg
      781 ccgacaagtc atcgctgatc acggtgcccg gcccaggcgt cggtgactgg gatctctcgc
      841 cggacgacaa ttacatcgtc tacgcatcga acgggtggga gccgcacgat gaagaaagtc
      901 cgacggatct ctggatccac gatcgcgcgg ctggatcgac acgtcgcgtg acgacgcgct
      961 ggggacctga gacgcagccg gttttctccc gtgacggtga atggatcgcc tacaaggccg
     1021 accaggacag cagcatcacc tactcccagg aagaactgcg ggtcgtgtca gtttccggac
     1081 ttgtggggca ccaggtatcc ggcgatctcg acctggctct cgataagccg ttctgggcga
     1141 ccggatcccg ggtgctcagt gccgaagtga gccagggcct ggacaatgga cgagtcgaat
     1201 tctcgcccgg ccctctctac ctgacattgc cggacgggag ccggatgcgc ggggatattc
     1261 cggctccgat cgaggccatg ctccaggcaa ccgtgggccg ctacgacagc gaattcagcg
     1321 ccgcacaaaa cgcttccgtg atcacatgga tcggcgagtc ctcgggtgaa tggcccgaag
     1381 tctggctgtc ctacgaggtt ggtcgcagtg gcaagatcag ccgggagggc cagtggccgg
     1441 atcctgctcc agtctcaagc cttgaaagtg gaacattcgg caagggtgtc ggtgactttc
     1501 gtcgcatccg gtcgacggcc cccgacggca ccgtcaccga atccctcctc gccctccccg
     1561 acaccaccct ctacggcccc ggcccctacc ccaccatcac ccaactccac ggcgggccct
     1621 ataaccgcaa caccgcccgc ccgcgcgaca accccaccgc gctggtcctt gccagccacg
     1681 gctacctcgt ctacatgccc aacttccgcg gcagcaaggg gtacggcaac gccttcggca
     1741 ccgcggcccg gggccgagac ttcgccgagg gcccgtaccg cgacgtcatg gccggcctcg
     1801 acagcctgat cgcccggggt ttggccgatt cgacgcggct cggtatcatg ggcggctcgt
     1861 acggggggta cctgtcgatg ttcgcgattt cgcggaccga ccggttcaag gcggcggttt
     1921 cgatgtacgg gatgtcgagc ctcattacgg acttcggcaa cgggaagtcg ccgtcgtacg
     1981 agctcgagta cctgggcgaa ccgtactgga aggggaatcc actctggacc gaactgtcgc
     2041 cggcctggca cgtcgacaag atccatacgc cgctcctgct cctgcacggg gagaacgacc
     2101 aatcggtggg catctcgaat tcacgggaga tgtggacggc cctgaccgtc ctgaagcgcc
     2161 cggtggagtt catcacctat ccgcgggagg ggcacggctt ccgcgagccg aatcaccgga
     2221 tcgatgcgtg tcggcggacg gtggcctggt tcgaccggta tttgaaatag gccggggcgc
     2281 gagactgttt cgctttcagg ccgccgtcgc cctgctcctc gccggcaccg ccgtcgcccc
     2341 ggtcgatcgc gccgtcgcgg caccaaccaa ccctgattcg ctgtcagccg aggcggccac
     2401 ccccgtcacc gtcctctacc agggacgcct gaccgacggc ggcggcaccg ccatccccgg
     2461 gccggtcact ctccaggtgg ccctctattc gtcggcttcg gcgggaaccc ctctctggac
     2521 cgaaacgcac ccgaacgttc cgcttactcc cgaggggatc tacagcatcc tgctcgggag
     2581 ccttacgccg tttccggcca acgcctggac ggatccggac cggtggctcg gcaccacggt
     2641 caatggaggg gatgaattgc agccccggat gcgggtggcc tcggtgccgt tcgcgatcga
     2701 ggccaaccgg ctgaacggga agcggtcgac cgactttgat ccgatcggtg cggcggacat
     2761 cgcggcggcg ggcgtgtcgg cccagctcaa ggggaacgat gccaccccgc cgaaccaggg
     2821 gtcgaaccag gtgcactgga acaacctgta cggaatgccg gacgggttca ccgatggtac
     2881 cgatgatctc ggggcgggcg tcacggatca cggcgcactg accgggctcg ccgacaacga
     2941 tcatccccaa tatctgctgg gggccgcggc ggccaccagc gacgggaatg cgcca
//
LOCUS       DASVOD010000611         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_664672, whole genome shotgun sequence.
ACCESSION   DASVOD010000611 DASVOD010000000
VERSION     DASVOD010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664672"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..682)
                     /locus_tag="VF720_13345"
     CDS             complement(47..682)
                     /locus_tag="VF720_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880394.1"
                     /translation="MNVSSVAAALEPVSRFGGVLVFLIEFGIIAVLFRAMGIFRDEVR
                     KVDSLVAEYVRCRDYLLSTRRPSVEEGWRTPDGGAFREAWGNFNNQLAIIRKRYRSSV
                     RNAHVLLIVFVVLLVLAIIVWMLGLPPADGPLRIITPLAAAAAGLFLWAAALTALRAQ
                     NTLIKTRTDGNYLIAPWPTDGGTPDRQLALMDKFLPVDTDRPDIDPATEVV"
     gene            complement(679..1191)
                     /locus_tag="VF720_13350"
     CDS             complement(679..1191)
                     /locus_tag="VF720_13350"
                     /inference="COORDINATES: protein motif:HMM:NF027592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HEX7880395.1"
                     /translation="MRLLHLVRLPALLRLSAPLRLPVAALFLLALAGCGPHHRDWSSY
                     GDPIAAEPEPVVIKASQLLGEPARFNGQTVVVEGEVIEVCQNKGCWMTFMDGDREMRI
                     RFKDYAFFVPKDCGGKKARVAGLFEVKDVPADEARHYLEDAGRHAEAEKITGPVPSYT
                     FMASGVMIES"
     gene            complement(1254..1634)
                     /locus_tag="VF720_13355"
     CDS             complement(1254..1634)
                     /locus_tag="VF720_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880396.1"
                     /translation="MKTVVGLLLLLLPPLLAGCSKGVDPAPPNMEGDWTIHNEATGVE
                     TILELDQDGTTLSGSWHRASSTIRVTGEITPTNEFDLTGRSNNDTIVFNAEANDGFSR
                     FTGTVSVYDVRGDRRSLQDVTGRR"
     gene            1784..3769
                     /locus_tag="VF720_13360"
     CDS             1784..3769
                     /locus_tag="VF720_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7880397.1"
                     /translation="MTSCDIKITNTTILTMDGEYTIHKTGSVAIVGSMIAAVGPDADA
                     YTAAQTVDAGGKILMPGLVNGHTHVPMTLLRGLADDLRLDVWLLGYVMPVEREFVSPE
                     FVTLGTKIAAAELIRSGCTCFADMYYFEESIAKATADAGLRAVCGQTILKFPSPDAAS
                     YEDSLALAREFIRKWKDHPLIVPAPAPHAPYTCTPEILQATADLAVEFDVPLLTHLSE
                     TLLEVENSRKERGMPVIPWVKKQRLLDAKLVAAHCVHIDEGEMRTLKNAGAGVVHNPS
                     SNLKLASGIAPVMKMLETGLNVGLGTDGPASNNDLDMFEEMRLAAFLAKGTTGDPTAL
                     PARLAMAMATRFGAKALHLGDIAGSIEVGKRADLILVDLSPLHNQPSFERDPNVLYSR
                     LVYASKSTDVSDVMCNGKWLMQDKRLRTLDEEELVREATDWARRIDTFLIAREESILQ
                     KLIGVGGAALGESFEVQVKARLGQDAPERVKAVLEGDALTVLRNVHYDQFDTYFVFND
                     PAAGRIRYRDDQFLNDKGEVTGSRARLTLTEPTREAEIGGVLLSRSRWFAPATQSLRF
                     YREYFEPAAEIEIEKNRRRWQVVFRGVEFYVNLDQIRKPAIDGWWLEIKSRTWSRRDA
                     EDKAALIHELLQLFGARADHTVREDYVELAVGTNGKG"
BASE COUNT          723 a         1345 c         1361 g          639 t
ORIGIN      
        1 ggctggagtt gacccccgaa gtaccggaga tctccccaga gtccgctcag acgacctccg
       61 tggccggatc gatgtcgggc cggtcggtgt ccaccggcag gaacttgtcc atgagggcga
      121 gctgccggtc gggcgtgccg ccgtcggtgg gccacggcgc gatcaggtag ttgccgtcgg
      181 tgcgcgtctt gatgagcgtg ttctgggcgc gaagcgccgt gagcgcggcc gcccagagga
      241 aaaggccggc cgccgccgcg gccagcggcg tgataatgcg cagtggcccg tcggccggag
      301 gaaggcccag catccacaca atgatggcga ggacgagcag cactacgaac acgatgagca
      361 ggacgtgcgc gttccggacg ctcgaacggt agcgcttccg gatgatggcg agctggttgt
      421 tgaagttgcc ccaggcctcg cggaaggcgc caccgtccgg ggttcgccac ccctcctcca
      481 ccgacgggcg acgggtggag aggaggtaat cgcgacagcg tacgtactcg gccaccaggg
      541 aatcgacctt ccggacttca tcgcgaaaaa ttcccatcgc ccgaaagagg acggcaatga
      601 tgccgaactc gatcaggaag accaggacac cgccgaaacg cgacaccggt tcgagggcgg
      661 cggccaccga cgatacgttc atgactcgat catcaccccg ctggccatga acgtgtagct
      721 cggcaccggg ccggtgatct tctcggcctc ggcgtgcctg cccgcgtctt ccaggtagtg
      781 ccttgcctcg tcggccggca cgtccttcac ctcgaagagc ccggccacac gggccttctt
      841 gccgccgcag tccttgggca cgaagaaggc atagtccttg aagcggatcc gcatctccct
      901 gtccccatcc atgaacgtca tccagcaacc cttgttctgg cagacctcga tcacttcccc
      961 ttccacgacc acggtctggc cgttgaagcg ggccggctcg cccaggagct gcgatgcctt
     1021 gatcacgacg ggctcgggct ccgccgcgat cggatccccg taggacgacc agtcgcgatg
     1081 gtgggggccg cacccggcaa gggcaaggag gaacagggcc gcgaccggga gtcgcagggg
     1141 tgcggagagt cgcaggagcg cggggagtcg cacgaggtgg agcaatcgca tggggacggc
     1201 ctccggtcgg agtggacgga aacggttgcc atcggagaat agcaaacggc gccctaccgc
     1261 cgaccggtca cgtcctgcag cgaacgccgg tcgccgcgca cgtcgtacac gctgaccgtg
     1321 ccggtgaagc gggagaagcc gtcgttggct tccgcgttga agacgatcgt gtcgttgttc
     1381 gaccggccgg tgaggtcgaa ctcgttggtg ggggtgatct cccccgtcac gcggatcgtc
     1441 gagctggccc gatgccagga gcccgagaga gtggtgccgt cctggtcgag ctcgaggatc
     1501 gtttcgacgc cggtggcctc gttatggatg gtccagtccc cttccatgtt cggcggggcg
     1561 gggtcgacgc ccttcgagca gccggcaagg aggggcggaa ggaggaggag gaggagcccg
     1621 acgactgttt tcatcatccc tccatcacgc gcgattcgaa gtactcgccc cgaagcgagc
     1681 aaaccgaacg acaccggata attccgccag gtcggaagag gggagtgccg atctgggcta
     1741 tactcgcaca actttttcga gcggacgagg gggggccgag cggatgacgt cctgcgacat
     1801 caagatcacg aacaccacca tcctgacgat ggatggggag tacacgatcc acaagaccgg
     1861 atcggtggcc atcgtcgggt cgatgattgc cgcggtgggg ccggacgcgg atgcgtacac
     1921 cgccgcccag acggtcgacg ccggcgggaa gatcctgatg cccgggctcg tcaacgggca
     1981 cacccacgtg ccgatgacgc tgctgcgcgg cctggccgac gacctgcggc tcgacgtgtg
     2041 gctcctcggc tacgtgatgc cggtcgagcg cgaattcgtg agcccggagt tcgtgacgct
     2101 cggcacgaag atcgcggcgg ccgaattgat ccgctcggga tgcacctgct tcgccgacat
     2161 gtactacttc gaggaatcga tcgccaaggc cacggccgat gccggcctcc gggcggtctg
     2221 cggccagacg atcctcaagt tcccctcccc cgatgcggcc agctacgagg actccctggc
     2281 gctggcccgc gaattcatcc gcaagtggaa ggaccatcct ctgatcgtgc cggccccggc
     2341 gccgcatgcc ccctacacct gcacaccgga aatcctgcag gccaccgccg acctcgcggt
     2401 ggaattcgac gtgccgctcc tcacccacct ctccgaaacg ctcctcgagg tggagaattc
     2461 gcgcaaggaa cgcgggatgc cggtcatccc ctgggtcaag aagcagcggc tgctggatgc
     2521 caagctggtg gcggcccact gcgtgcatat cgacgagggg gagatgcgca cgctcaagaa
     2581 tgcgggggcc ggggtggtgc acaacccgtc gagcaacctg aagctggcct cgggtattgc
     2641 gccggtgatg aagatgctcg agaccggcct caacgtgggc ctcggcaccg acggcccggc
     2701 cagcaacaac gacctcgaca tgttcgagga gatgcgcctg gccgccttcc tggccaaggg
     2761 aaccacgggc gatccgacgg ccctgccggc ccggctggcc atggccatgg ccacgcgctt
     2821 cggcgcgaag gcgcttcacc tgggggacat cgccggctcg atcgaggtgg ggaagcgcgc
     2881 cgacctgatc ctggtggacc tctctcccct ccataaccag cccagcttcg agcgcgaccc
     2941 gaacgtcctg tattcgcggc tggtttacgc ctccaagtcg accgacgtgt ccgacgtgat
     3001 gtgcaacggc aagtggctga tgcaggacaa gcggctccgg acgctggacg aagaggaact
     3061 ggtgcgcgaa gccaccgact gggcgcgccg catcgacacg ttcctgatcg cccgcgagga
     3121 gtcaatcttg cagaagctga tcggcgtggg cggcgcggct ctcggcgaga gcttcgaagt
     3181 gcaggtgaag gcgcgcctgg gccaggacgc ccccgagcgg gtcaaggccg tgctggaagg
     3241 ggatgcgctg accgtgctcc gcaacgtgca ctacgaccag ttcgatacct atttcgtctt
     3301 caacgacccc gccgccggcc gcatccgcta ccgcgacgac cagttcctga acgacaaggg
     3361 cgaagtgacc ggcagccgcg cgcgattgac cctgaccgaa cccacccgcg aggccgagat
     3421 cggcggcgtg ctcctctccc gctcccgctg gttcgccccg gccacccaga gcctccgctt
     3481 ctaccgcgag tacttcgagc cggcggccga aatcgagatc gagaagaatc gccgccgctg
     3541 gcaggtcgtg ttccgcggcg tcgagttcta cgtgaacctc gaccagatca ggaagcccgc
     3601 catcgacggc tggtggctcg aaatcaagag ccgcacctgg agccgccgcg acgccgagga
     3661 caaggcggcc ctgatccacg aactgctgca gctgttcggg gcgcgagccg accatacggt
     3721 gcgcgaggat tacgtggaac tggcggtggg gacgaacggg aaggggtagg ggcgccgcgg
     3781 gttgcgccgc ggcaggctcg gccgcgcggt tattgatttc gatcgacgtc ttgcaacgca
     3841 agacaactgc cgcaactttc gccgcgcctg ccccgatgac cctgtatcgg accctgttat
     3901 gttgcgctga tcccgatccg gggctatctt tgatgtggcc gcccggcatt cgtcggtccc
     3961 ccttggcctc tccgagtccg aacgcaccag gaggatcgga accatgccgc tcatcccccg
     4021 ccgccggttg ccgtccctga ccacgccccg caattggctc gtgcccgg
//
LOCUS       DASVOD010000612         4605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_612946, whole genome shotgun sequence.
ACCESSION   DASVOD010000612 DASVOD010000000
VERSION     DASVOD010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612946"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VF720_13365"
     CDS             <1..325
                     /locus_tag="VF720_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7880398.1"
                     /translation="LAVTISSSIQVHPDMEVRAEFMTTLGVDSLSYPEERVVSIGRNA
                     GFPVLVLAPRLGAWAKSRHAWLHGFPDTRPGYGHWNGLGHRLAGEALAEELARLFGKS
                     ACPTG"
     gene            401..2245
                     /locus_tag="VF720_13370"
     CDS             401..2245
                     /locus_tag="VF720_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HEX7880399.1"
                     /translation="MNILGLSAFYHDSAACLVQNGILTAAAQEERFTRVKHDYRFPSR
                     AIEYCLAEAGLAPEQIDYVAFYDKPLLKFERLLETYIAGAPAGFKLFRMGMPLWLREK
                     LHLPREMDKGLGGRYKGRYVFTEHHESHAASAFFPSPFDEAAILTLDGVGEWATGSIG
                     KGEGNRIHLLKEMRFPHSLGLLYSAFTYFTGFKVNSGEYKLMGLAPYGRPSYVDRIMD
                     KLIDLKPDGSFRMDMSYFAFHVKDVMTGPKFDALFDGPARAAEAPLTQREMDIAASIQ
                     QVTEEIMLRSARHAHELTGSRNLVMAGGVALNCVGNGKILREGPFEKIWIQPAAGDAG
                     GALGAALFTWHQLLDQPRQPRGKDLQRGSLLGPDILPADVQKLLDSVGAVYRRIDEES
                     ELEAAVADLIAREMVVGHVAGRMEFGPRALGNRSIIGDARSAKMQSVMNLKIKFRESF
                     RPFAPVVLRESVADWFDMRPDEDSPYMLLVAPVREDKRVANGNGTGESKNAWGIELLN
                     EIRSVVPAITHVDYSARVQTLDEERHGRFYRIVKRFHAATGCPVIINTSFNVRGEPIV
                     CTPRDAWRCFMATDMDALVLDRFILLKSEQPERAGVDRDAYLKEFKLD"
     gene            2238..2678
                     /locus_tag="VF720_13375"
     CDS             2238..2678
                     /locus_tag="VF720_13375"
                     /inference="COORDINATES: protein motif:HMM:NF039992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HEX7880400.1"
                     /translation="MIDLNLKPDRKHLRDFGWVALVVFGLLGAVVTWKHAIVGIHLSE
                     PATRTTAMVLWGLGALSGLLSMVRPEWNRFLYIGLMLAAFPIGFVVSHVIIAIIFFVI
                     ITPVGLVFRLMGRDALSRRFDRNAPSYWVDKKPVESVERYFRQF"
     gene            complement(2675..3943)
                     /locus_tag="VF720_13380"
     CDS             complement(2675..3943)
                     /locus_tag="VF720_13380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7880401.1"
                     /translation="MDSAARAGRPGSRKLLRGSLRHRLLISLAGLSALAVGSLACVYA
                     ITESVIERSALRNEMTEEIHYLIEWEHADRGAPPLSATLSYFPSESAPPDLRDLPPGT
                     FKRLHRQDRTVQVLTAADERGRTHVLVQDLGHETEREKSLLLSLLAGVATAAAGAWWV
                     SARLARHILSPLTELVGQIAEVDPLAPARRPVARTRDAELDPIPDAVNKLIGELDHVL
                     SRERAFADAASHELRTPLAIIRGAVDLLRERGDSPAHIVDRLDRAARRAQEDLGALLS
                     MSPSREPAAPHPVDLRVLLPAAADPYLREGNAGTRVVWEWGVSAEASLEPAALAIVFA
                     NLLRNALRAAPDGEVRIRADNDRIQIVDDGEGLPEGWPTYGEPRGRGLGLAIARILAE
                     RNGWQVRVEPGSRGGTEATLNFRAEGVSVG"
     gene            complement(3936..4604)
                     /locus_tag="VF720_13385"
     CDS             complement(3936..4604)
                     /locus_tag="VF720_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015278176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7880402.1"
                     /translation="MLVVEDDPDLAANLGDFLELRGHSVDFAATAHQGKELAGAQRFD
                     AIVLDRLLPGVDGADLCRALRASGHGTPILMLTALDKVEQRVEGLAAGADDYLVKPFA
                     MDELMARLDALHRRATRRVAPAGLRLADLEYDPATMIATRANRQLDLSPSVRKILEYL
                     LRHQDRVVTRTELCELLWGDPEVDALRGHIHELRRALDRPFGRPLLHTMRGGGWRLME
                     TDRG"
BASE COUNT          748 a         1581 c         1529 g          747 t
ORIGIN      
        1 cctggccgta acgatctcga gcagcatcca ggtgcacccg gacatggaag ttcgcgccga
       61 attcatgacc acgcttggcg tcgacagcct gtcgtatccg gaggaacggg tcgtgagcat
      121 cggccgaaac gcgggatttc cggtcctggt cctggcgccg cgcctgggcg cctgggcgaa
      181 atcgcgccac gcgtggctgc acggatttcc cgacacgcgt cccggctacg gtcactggaa
      241 cggcctgggc catcgactgg ccggcgaagc gctcgccgag gagctggccc gactcttcgg
      301 gaaaagtgcc tgcccgaccg ggtgatatac tcgctggcgc cccgacccgc gccgccctgc
      361 cccgggatgc ggtgatcgca gttcgccaga tggaatccgg atgaacatcc tcggactgtc
      421 agccttctat catgacagcg cggcctgcct cgtccagaac ggcatcctga ccgccgccgc
      481 ccaggaagaa cgcttcaccc gcgtgaagca cgactaccgg tttccctccc gcgccatcga
      541 atactgcctg gccgaggccg gcctcgcgcc ggaacagatc gattacgtgg ccttctacga
      601 caagccgctc ctcaagttcg aacgtctcct cgagacgtac atcgccggag ccccggccgg
      661 cttcaagctc ttccgcatgg ggatgccgct gtggctccgc gagaagctcc acctcccgcg
      721 cgaaatggac aaggggctgg gcggacgcta caaggggcgc tacgtcttca cggaacatca
      781 cgaatcgcac gccgccagcg ctttcttccc gagcccgttc gacgaggccg ccatcctcac
      841 gctggacggc gtgggagagt gggccaccgg ctcgatcggc aagggagaag ggaaccgcat
      901 ccacctcctc aaggaaatgc gctttcccca ctcgctcggc ctcctttact cggccttcac
      961 ctacttcacc ggcttcaagg tgaattcggg cgagtacaag ctgatgggac tggcgccgta
     1021 tggccggccc agctacgtcg accgcatcat ggacaagctg atcgacctga aacccgacgg
     1081 ctccttccgg atggacatgt cgtactttgc cttccacgtg aaggacgtga tgaccgggcc
     1141 gaagttcgac gccctcttcg acggcccggc ccgcgcggcc gaggccccgc tcacgcagcg
     1201 cgagatggac atcgccgcct ccatccagca ggtgaccgag gagatcatgc tccgctccgc
     1261 gcggcatgcc cacgaactga ccggcagccg gaacctcgtc atggcgggcg gcgtggccct
     1321 gaactgcgtg gggaacggaa agatcctccg cgagggaccg ttcgagaaga tctggatcca
     1381 gccggccgcc ggcgacgcgg ggggcgcgct cggcgccgcc ctcttcacct ggcaccagct
     1441 cctcgaccag ccccgccagc cccgtggaaa ggaccttcag cggggctcgc tcctcgggcc
     1501 ggacatcctg ccggccgacg tccagaagct gctcgattcg gtgggcgccg tctatcggcg
     1561 catcgacgaa gaatcggagc tcgaagctgc cgtggccgac ctgatcgccc gcgagatggt
     1621 ggtgggacac gtggccggcc gcatggaatt cggcccccgg gcgctcggca accgctccat
     1681 catcggcgat gcgcggagcg cgaagatgca gtcggtaatg aacctgaaaa tcaagttccg
     1741 cgagtcgttc cgaccgttcg cgccggtggt cctccgggaa agcgtggccg actggttcga
     1801 catgcgcccc gacgaagaca gcccctacat gctcctcgtg gcgcccgtcc gggaggacaa
     1861 gcgggtggcc aacggtaacg gaaccggcga gtcgaagaac gcctggggaa tcgagctgct
     1921 gaacgagatc cgctcggtgg tgccggcgat cacgcacgtg gactactccg cccgcgtcca
     1981 gacgctcgac gaggaacggc acggccggtt ctaccggatc gtgaagcggt tccacgcggc
     2041 caccggctgc ccggtgatca tcaacaccag cttcaacgtg cggggcgagc cgatcgtgtg
     2101 cacgccacgc gatgcctggc gctgcttcat ggccaccgac atggatgcgc tggtcctcga
     2161 ccgcttcatc ctcctgaagt cggagcaacc cgagcgggcc ggcgtggatc gcgacgccta
     2221 cttgaaggag ttcaagcttg attgacctga acctgaaacc cgaccggaag catctccgtg
     2281 atttcggctg ggtggcgctg gtcgtcttcg ggctcctcgg cgcggtggtc acctggaagc
     2341 acgccatcgt ggggatccat ctctcggaac cggccacgcg gacgacggcg atggttctct
     2401 ggggcctcgg ggcgctttcc ggcctcctgt cgatggtccg gccggagtgg aaccgatttc
     2461 tctacatcgg cctgatgctg gcggcgttcc ccatcggctt cgtggtctcc cacgtcatca
     2521 tcgcgatcat cttcttcgtg atcatcacgc cggtcggcct cgtgttccgc ctgatgggac
     2581 gcgatgcgct gtcgcgcagg ttcgaccgga acgcccccag ctattgggtg gacaagaagc
     2641 cggtcgaatc ggtcgagcgc tacttccgcc agttctagcc gaccgaaact ccttcggccc
     2701 ggaagttcag cgtggcttcg gtgcctcccc ggctgcccgg ctccacccgg acctgccagc
     2761 cgttccgctc cgccaggatg cgcgcgatcg cgaggcccag cccccggccg cgcggttctc
     2821 cataagtcgg ccaaccctcc ggcagccctt caccgtcatc gacgatctgg attcggtcgt
     2881 tgtcggcccg gatccttacc tccccgtccg gggcggcccg aagcgcgttc cgcaggagat
     2941 tcgcgaagac gatcgcgagc gccgccggtt ccaggctcgc ctcggcgctc acgccccact
     3001 cccacaccac gcgcgtgccg gcgtttccct cacggaggta aggatcggcg gcggcgggga
     3061 ggagcaccct gagatccacc gggtgcgggg ccgccggttc acgcgacggg gacatggaaa
     3121 gaagcgctcc gagatcctcc tgggccctcc gggccgcgcg gtcgagccgg tcgacgatgt
     3181 gggccggcga gtcgccacgc tcccggagga gatccaccgc tccgcggatg atggccagcg
     3241 gtgtccgcag ttcatggctg gccgcatcgg cgaaggcgcg ctcgcgggag aggacatggt
     3301 ccagctcgcc gatcagcttg ttcacggcgt ccggaatcgg gtcgagctcc gcgtccctcg
     3361 tgcgtgccac cggccgccgg gcgggcgcca ggggatcgac ttcggcgatc tggccgacca
     3421 gttcggtgag tgggctgagg atgtggcggg cgagccgggc cgaaacccac caagctccgg
     3481 cggccgcggt ggccacaccg gcgagcagcg aaagaaggag cgatttctcc cgttcggttt
     3541 cgtgcccgag gtcctggacg aggacgtggg tgcgcccccg ttcatccgcc gcggtcagga
     3601 cctgcacggt gcggtcctgc cggtgcagtc gcttgaaggt gcccggaggg aggtcccgga
     3661 ggtcgggtgg ggccgattcg gatgggaagt agctcagggt tgcggagagc ggtggagcac
     3721 cgcggtcagc gtgctcccac tcgatcaggt aatggatctc ctccgtcatc tcgttccgga
     3781 gcgccgacct ctcgatcacc gactcggtga tggcgtacac gcatgccagg cttccgaccg
     3841 cgagtgccga cagcccggcc aggctgatca gcagccgatg gcggagtgaa ccccgaagga
     3901 gcttgcgtga cccgggcctg ccggcccggg ccgcactatc cacgatcggt ttccatcagc
     3961 cgccatcctc cccccctcat cgtatggagg aggggccttc caaagggccg gtcgagcgcc
     4021 cgccgcaact cgtggatatg cccgcggagc gcgtcgacct ccggatctcc ccagaggagc
     4081 tcgcagagtt cggtgcgggt gaccactcga tcctggtgcc tgagcaggta ctcgaggatc
     4141 ttgcgcacac tggggctcag gtcgagctgc cggttggcgc gcgtggcgat catggtggcc
     4201 gggtcgtact cgaggtcggc cagtcgaagc ccggccggcg ccacccggcg ggtggcccgg
     4261 cggtgaaggg catcaaggcg cgccatgagc tcgtccatgg cgaacggctt cacgaggtag
     4321 tcgtcggcgc cggcggccag cccctccacc cgctgctcaa ccttgtcgag cgcggtcagc
     4381 atcaggatgg gagtgccatg tccggacgcg cggagcgcgc ggcagagatc ggctccatcg
     4441 accccgggga gaaggcggtc gagcacgatg gcgtcgaatc gctgggcgcc ggccagctcc
     4501 ttcccctgat gggccgtggc ggcaaagtcc accgaatgac cgcggagctc gagaaaatcg
     4561 cccaggtttg ccgccaggtc cggatcatcc tccacgacga ggatc
//
LOCUS       DASVOD010000613         3853 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1034800, whole genome shotgun sequence.
ACCESSION   DASVOD010000613 DASVOD010000000
VERSION     DASVOD010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034800"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1767)
                     /locus_tag="VF720_13390"
     CDS             complement(<1..1767)
                     /locus_tag="VF720_13390"
                     /inference="COORDINATES: protein motif:HMM:NF019145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880403.1"
                     /translation="MRLTPGSSPLFLAILLAVLIASPRDARAWEPDPDHDAYAVDRRG
                     VEPGLSAKAVRSARLDAGNGWTEFVRQHPGWVAGWNSASGRLMNATGPAAGSMNSATG
                     APSTGPAGRLAATEVEAKARALLDGELKSWFPTDDQLEFVRAIPAGDRWWVHFGQRVG
                     GVPVWNARVSMHLLADGRVNRIVNRAFPDAAIDPNPTVAPAEAARIAAQDLPAGAESA
                     GSPELVILPIERGRTTEYRLAWKAEWRTMEPAGNWMSFVDARNGELIWRFNAGHHAEL
                     TGKITADIEPVTQGDPVVETPMRHLSFVAAGGGLVPPEIHTTDVNGDYTLSSPTDIGR
                     QLNVLLGGPYGVVFDAANAFATPNVLFTVPETSPLDFPLHLTGTQLPLPGMDAFHHAM
                     LAHDYVKALDPSLIAIDFAAPIVVNFPASGVCNAFWNGTQLVFFTAGNQCANSARVAT
                     VVMHEYGHLVTDLQYRPFFPSGAMHEGFSDYLACSMTNQPIVGPGWRPSSTPDYIRRI
                     DIDRRTPDDVTGETHNDGLIIASTLWDLREIYGAALIDSLWHYARYGYADNFDDYFVD
                     FLLTDDDNGDIYDGTPHFGPVVD"
     gene            complement(1879..2217)
                     /locus_tag="VF720_13395"
     CDS             complement(1879..2217)
                     /locus_tag="VF720_13395"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020478792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX7880404.1"
                     /translation="MSEPHHEAFPGMLNGGIIGALLDCHSNWTAAWHLMNRAGMDHPP
                     CTVTADFHVVLKRPTPSGAPVHLAARAVESTDDRAVIEATLTAGGKICATCRGTFVAV
                     QPGHPAYHRW"
     gene            2519..3127
                     /locus_tag="VF720_13400"
     CDS             2519..3127
                     /locus_tag="VF720_13400"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880405.1"
                     /translation="MPSIRFRKALSGVLLFLLAATPMAGPAFAQEPAAKPAAAVPGVK
                     GDMLFWIQDAESKLIELAEAIPADKYSWKPAEGVRTIAQVLMHAATANLGIPTFIGVK
                     PPEGFDFRTYETSTTDKAEVIKRLKASFEHTRNAVLNIKDADMDNPVDLFGNKTTVRG
                     TAMLFVTHNHEHLGQMIAYARSVGVTPPWTVREQAAMKEGGK"
     gene            complement(3167..>3853)
                     /locus_tag="VF720_13405"
     CDS             complement(3167..>3853)
                     /locus_tag="VF720_13405"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7880406.1"
                     /translation="DKVMAKKIFEFHKIPTPNFATVWRGRMDHAHDINFPVIVKPARE
                     DGSIGIPFSAYVESIKELMTRVDELHAEFDTPVLVEEYVEGREIYVGVLGNKKPEALP
                     PIELDLSKLPEGTPKIAGTEVKWERSTEAYKTTQPRVAEDLDEKTVALLQETGITAFQ
                     ALGLRDYARIDFRLDKKKRPFVLEVNPNPYLLSTAELSLSFKHTGRSYADLVGEIVKA
                     AAERYGLVLS"
BASE COUNT          636 a         1237 c         1332 g          648 t
ORIGIN      
        1 atcgaccacg gggccgaagt gcggcgtgcc gtcgtagatg tcgccgttgt cgtcgtcggt
       61 cagcaggaag tcgacgaagt agtcgtcgaa attgtccgcg tagccgtagc gcgcgtaatg
      121 ccagagcgag tcgatgaggg ccgccccgta gatctcgcgc agatcccaga gcgtggaggc
      181 gatgatgagg ccgtcgttgt gcgtttcgcc ggtcacgtcg tccggcgtgc ggcgatcgat
      241 gtcgatccgc cggatgtagt cgggcgtgga gctggggcgc caccccggcc cgacgatcgg
      301 ctggttggtc atcgagcagg ccaggtaatc ggagaagccc tcgtgcatgg cgcccgacgg
      361 gaagaagggg cggtattgca ggtccgtcac caggtgcccg tactcgtgca tgaccaccgt
      421 ggccacccgc gccgaattcg cgcactggtt gcccgccgtg aagaagacca gctgcgtccc
      481 gttccagaag gcattgcaga cgccgctggc cggaaagttc acgacgatcg gggccgcgaa
      541 gtcgatggcg atgagcgacg ggtcgagcgc cttcacgtaa tcatgcgcca gcatggcgtg
      601 atggaaggcg tccatgccgg gaaggggaag ctgcgtgccg gtcaggtgga gcgggaagtc
      661 gagcgggctc gtctccggca ccgtgaagag aacgttgggc gtggcgaacg cgttggcggc
      721 gtcgaacacc acgccgtagg ggccgccgag caggacgttg agctgacgtc cgatgtcggt
      781 cgggctgctg agggtgtaat cgccgttcac gtccgtcgtg tggatctccg gcgggacaag
      841 cccgccgccg gccgccacga acgagaggtg ccgcatcggc gtctcgacca ccggatcgcc
      901 ctgggtgacg ggctcgatgt cggcggtgat cttgccggtc agttcggcgt gatgcccggc
      961 gttgaaacgc cagatgagct cgccgttccg cgcgtcgacg aacgacatcc agttgccggc
     1021 cggctccatc gtgcgccact cggccttcca ggccagccgg tactcggtgg tgcggccgcg
     1081 ctcgatcggc aggatgacca gctcgggcga accggcgctc tccgcaccgg ccggcagatc
     1141 ctgcgcggcg atgcgtgccg cctcggccgg cgccaccgtg ggattcggat cgatcgccgc
     1201 atccgggaag gcgcggttga cgatgcggtt gacccgccca tcggccagga ggtgcatcga
     1261 gacacgggca ttccagaccg gcacgccgcc caccctctgg ccgaagtgga cccaccagcg
     1321 atcccctgcc ggaatggccc gcacgaactc gagctggtcg tcggtcggga accatgactt
     1381 gagctcgccg tcgagcaggg cgcgtgcctt tgcctccacc tccgtggcag cgagtcggcc
     1441 ggcgggtccc gtggacgggg ctccggtggc ggaattcatc gacccggcgg cgggacccgt
     1501 ggcattcatc aaccgcccgc tcgcggagtt ccatcccgcc acccagccgg ggtgctgccg
     1561 gacgaattcc gtccagccgt tccccgcgtc gagccgggcc gagcgaaccg ctttcgccga
     1621 cagcccgggc tccacccccc ggcggtcgac cgcgtaggca tcgtggtcgg gatcgggttc
     1681 ccaggcgcgg gcgtcacggg gcgaggcgat caggacggca agaaggatgg caaggaagag
     1741 cggcgaactt cccggggtca atcgcacggc aggtccctcg aggcggtgga agtgagtgtt
     1801 cgggcgcccg ctatcgacgg atcagggcgc gatggagttc agcggtaatg gcgatttggt
     1861 atcgagggga ttgggatatt accaccggtg ataagccgga tgtcccggct ggaccgccac
     1921 gaaagtgccg cggcaggtcg cgcatatttt tccgcccgcc gtcagcgtgg cttcgatcac
     1981 ggcccggtcg tccgtcgatt cgactgcacg tgcggccagg tggaccggcg cccccgaggg
     2041 ggtggggcgc ttcaggacaa cgtgaaagtc agcggtgacc gtacaggggg gatgatccat
     2101 accggccctg ttcatcaggt gccaggccgc cgtccagttg gagtggcagt cgagcagcgc
     2161 cccgatgatc ccgccgttca gcatgccggg aaaggcctca tggtggggct cggacatcca
     2221 atcgcacacg acttcctcgc cgccgggggc tggaaaactg cggatgcgta atcctttctc
     2281 attggcgggg ccgcatccga agcaggcgtt cttcggcgcg tagcgctcct ggaggctggg
     2341 accggcggac ggcgtatggg cggggggctg ttcgctcatg agggcgctga ctatatcgcg
     2401 cccgttaccc gccggaaagc cgaattggat acatgttgac cctctccgtc cgccgggtac
     2461 aggatggcga ccgagttcgc cgttccgtgc ccgacccaac cccaaggagg ccgtaccaat
     2521 gccctcgatc cgattccgga aggcgctatc cggtgtgctg ctcttcctcc tggccgccac
     2581 cccgatggcc ggcccggcgt tcgcacagga gccggccgcc aagccggccg cggccgttcc
     2641 cggcgtgaag ggggacatgc tcttctggat ccaggacgcc gagtccaagc tcatcgagct
     2701 ggccgaggcg atcccggcgg acaagtattc gtggaagccg gccgagggtg tccgcaccat
     2761 cgcgcaggtc ctgatgcacg ccgccacggc caacctcggc atccccacct tcatcggcgt
     2821 gaagccgccc gagggattcg acttccggac ctacgagacc tccaccaccg acaaggcgga
     2881 agtgatcaag cggctcaagg cctccttcga gcacacgcgg aacgccgtgc tcaacatcaa
     2941 ggacgccgac atggacaacc cggtcgacct cttcggcaac aagaccaccg tccgcggcac
     3001 ggccatgctc ttcgtcaccc acaaccacga gcaccttggg cagatgatcg cgtatgcccg
     3061 ctcggtgggg gtgacgccgc cgtggacggt gcgggagcag gcggcgatga aggagggggg
     3121 gaagtaggtt tgacggcgag gcggcggatc gccgtctcgc gaaccgtcag ctcaatacga
     3181 gcccgtagcg ctccgccgcc gctttcacga tctccccgac caggtcggca taacttcgcc
     3241 ccgtatgctt gaacgacaac gacagctcgg cggtggaaag tagatacgga ttcggattca
     3301 cctccagcac gaaggggcgc ttcttcttgt ccagccggaa gtcgatgcgg gcgtagtcgc
     3361 gcaggccgag cgcctggaag gcggtgatac cggtttcctg cagcagagcc accgtctttt
     3421 cgtcgaggtc ctcggccacc ctcggttgtg tggtcttgta ggcctcggtg gagcgctccc
     3481 acttgacctc ggtgccggcg atcttgggcg ttccttccgg cagcttggag agatcgagct
     3541 cgatcggcgg cagcgcttcg ggcttcttgt tgccgagcac gcccacatag atctcgcgcc
     3601 cctccacgta ctcctccacg agcacgggcg tatcgaactc ggcgtggagc tcgtcgacgc
     3661 gcgtcatcag ttccttgatg ctctcgacgt aagccgagaa ggggatgccg atcgagccgt
     3721 cctcgcgggc gggcttcacg atcaccggaa agttgatgtc gtgggcgtgg tccatccggc
     3781 ctcgccagac ggtggcgaag ttgggggtgg ggatcttgtg gaattcgaag atcttcttgg
     3841 ccatcacctt gtc
//
LOCUS       DASVOD010000614         4199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_925936, whole genome shotgun sequence.
ACCESSION   DASVOD010000614 DASVOD010000000
VERSION     DASVOD010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925936"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1354
                     /locus_tag="VF720_13410"
     CDS             <1..1354
                     /locus_tag="VF720_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX7880407.1"
                     /translation="TAGAEGNVTIHDAKTLAQDSTFPAHARRVYSMAWSLDATRLATG
                     SIDRTARVWQVPDGRLVTTFSEHHGTVAALAFDPSGGRILSTGSDNRLFLWNAATGAI
                     LSSFRGHESDVSAIAAGAGPDVVLTGDWGGVIRRWTWRTEDVRTLGYPHTQFEISALN
                     QAAVDPLEEHIACATSVRDAPVWSFDAPLPERLIPLEAGRCMAYTPDGAFLIAGTDKG
                     SVRLIRAADGTTLRSIGAHDGPVFGMALNPSGDLVATTSGDSLIRFWTVPALKPADSI
                     AAGINLRGLAFSPSGDLLAGAGTDGAVRLWSTLPTRLVERIASPSPRLTALAFDSSGQ
                     RFAAAGSDGALYLIDPATKRVTLIREAGPAPLTSVAWSRDGTRIAAGALDETVRLFDP
                     EKRREVLSLHGHAGKVTSVLFLRNDRALISTSTDGTVRIWEAGVTGRRGNASETAGLQ
                     "
     gene            1425..2459
                     /locus_tag="VF720_13415"
     CDS             1425..2459
                     /locus_tag="VF720_13415"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7880408.1"
                     /translation="MRSTTIAALLLALVACQAHAGTPTVKLDGFLELTKGSYLIVDGQ
                     RVVTSGSTKLKGTRSASGIPLGSIMKVKGTRDSLGTILATELEAKPNRIEGSEAKLFA
                     GAGMAESTWLAAGKIVETDENGKEQSMGALRTSGPDYDRVRRIVDGVMPPYIKRSDVR
                     VYVVENKEWNAMAMPNFAVYVFTGIINDLNDDELALVLGHEIAHATNEHSRRQSSKST
                     FSAIAGQVALFGASQIDNSTVRALSQQATSLAYSAFNNNYSRDYEDQADRVGMRYAYE
                     GGYNAKVGPDLWRKFAARYGDMPKIQNYFYGNHSTSGDRARNLDREIRMNYTGAIDPP
                     TSGGKAPGKK"
     gene            2854..>4199
                     /locus_tag="VF720_13420"
     CDS             2854..>4199
                     /locus_tag="VF720_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880409.1"
                     /translation="MNFPILARHSRVHHRVRRLQMLAVTAVVLACGASSARAQYTNLY
                     GNAGTDGGGTQTQQHPDDPNATLSGLMSQDAVGNEASGNGMLSAWDGKFRVSAHACSG
                     GGPGSGGTANGQATCYVYQDYQVVSSTLPAGTPVMIRINWAVSGKETGAGTVTHYFYG
                     AVGGVSGQIVITVNGVSILNVSGERRRHITSEGFSHYTTGTLNVEEDSLTHFVPVPVG
                     QVVRIFMTGTAAGSSTAGASSVVDGDSQLAMLWGIASLDPNASVISLTNVGHTPPPPQ
                     NATLPNAVDYRAPRPVGLLECFTFSEQPVEAIACASGDAGFAVDVSGTGPFTYQWYKN
                     GVALNGLANPSATTASLMLPDVTAGDAGSYTVKITNPCGDRTSDAAMLTVTPPPTIST
                     HPASTSVSPPTMAQVSVVANGPVDDYQWQIETAPGVWADLSSKSLALPCGGAALTDT"
BASE COUNT          732 a         1456 c         1323 g          688 t
ORIGIN      
        1 caccgcggga gccgagggaa acgtcacgat ccacgacgcg aagacccttg cgcaggactc
       61 gacctttccg gcccatgcgc gccgtgtgta cagcatggcc tggagcctgg atgccacccg
      121 gctggccacc ggctccatcg atcggacggc ccgcgtctgg caggtgccgg acggtcgtct
      181 cgtgaccacg ttcagcgagc atcacggcac cgtcgccgcg ctcgctttcg atccctccgg
      241 cggccggatc ctctccacgg gctccgacaa ccggctcttt ctctggaacg cggccacggg
      301 cgccatcctg tcgtcctttc gcggacacga gtccgatgtc tcggccattg ccgcgggagc
      361 cggtccggat gtcgtcttga ccggggactg gggaggcgtc atcaggcgct ggacatggcg
      421 cacggaagac gtgcgcacgc tcggttatcc gcacacgcag ttcgagattt ccgcgttgaa
      481 ccaggcggcc gtcgatccgc tcgaggagca catcgcctgc gccaccagcg tgcgtgatgc
      541 cccggtctgg tccttcgatg cgcctctgcc cgagcgcctc atcccgctgg aggccggccg
      601 ctgcatggcg tacacgccgg acggcgcctt tctcatcgcc ggtacggaca agggatcggt
      661 ccggctcatc cgcgccgccg acggcacgac gctccggagc atcggcgctc acgacggccc
      721 cgttttcggc atggcgctga atccatccgg cgacctcgtg gccacgacct cgggcgactc
      781 gctgatccgg ttctggaccg ttccggcgct caagccggct gactccatcg ccgccggcat
      841 caacctccgt gggctggcct tttcaccgtc cggagatctg ctggcggggg ccggcacgga
      901 cggcgccgtc aggctctgga gtacgctccc gactcgactg gtcgagcgca tcgcgtcacc
      961 ctcgccgcgc ctcaccgccc tggcattcga ttcctccggg cagcggttcg ccgctgccgg
     1021 ctccgatggc gccctctacc tgatcgaccc cgccacgaaa cgggtgacgc tcatacgtga
     1081 ggccggcccc gcgcccctca cctccgtcgc ctggagccgc gacggtacgc gcatcgccgc
     1141 cggcgcgctc gatgaaaccg tccgcctctt cgaccccgaa aagcgccgcg aagtcctgag
     1201 cctccacggc cacgccggca aagtcacctc cgtccttttc ctgagaaatg accgggcgct
     1261 gatctcgacc tcgaccgatg gcacggtcag gatctgggag gcgggggtga cggggaggag
     1321 gggcaacgca tcggagactg ccgggcttca gtgagcacga ccatgtgata gcctgcccgg
     1381 taatcggccc ccagagggat gaccccggcc caagggaggg attcatgcgt tccacgacaa
     1441 ttgcggccct gctcctggcg ctcgttgcct gccaggcaca tgccggaact cccactgtca
     1501 aactcgatgg cttcctcgag ctcaccaagg ggagctacct gatcgtcgac ggccagcgcg
     1561 tcgtgacgtc gggttcgacg aagctcaagg gcaccaggtc cgcttccgga attccgctcg
     1621 gctccatcat gaaggtcaag gggacgcgcg actccctggg gaccatcctg gccaccgagc
     1681 tcgaggcaaa gccgaacagg atcgagggga gcgaagcgaa actcttcgcc ggcgccggca
     1741 tggccgaatc cacttggctc gcggccggaa agatcgtgga gacggatgaa aacggcaagg
     1801 agcagagcat gggtgccctg cgcacctccg gtcccgatta cgaccgcgtg cgccgcatcg
     1861 tggatggggt catgcccccg tacatcaagc gttccgacgt ccgcgtctat gtcgtggaga
     1921 acaaggaatg gaacgcgatg gccatgccca acttcgcggt ctacgtcttc acgggaatca
     1981 tcaacgacct gaacgacgac gaactggcgc tcgtcctcgg gcacgaaatc gcccacgcca
     2041 ccaatgagca cagccgccga cagtcgagca agtccacctt cagcgcgatc gcggggcagg
     2101 tggcgctctt cggcgcctcg cagatcgaca acagcacggt ccgcgccctt tcgcaacagg
     2161 ccacctcgct cgcgtattcc gccttcaaca acaactacag tcgcgactac gaggaccagg
     2221 ccgaccgagt cggcatgcgt tacgcctatg agggaggcta caacgcgaag gtgggtcccg
     2281 atctgtggcg gaaattcgcg gccaggtatg gcgacatgcc gaaaatccag aactatttct
     2341 acggcaacca ctccaccagc ggcgatcggg ccaggaatct ggaccgcgag atccggatga
     2401 actacacggg cgccatcgat ccgccgacga gcggggggaa ggcgccgggc aagaagtagg
     2461 ccgcggctcg ggccatcgag tgtcaaccgt caggcggccg cccgggccga aaaggggtga
     2521 ttttgcttgt ttggccccta tcgcgtctct atgctctctt atggccgcat aaagttccgt
     2581 cggcactacc ggcggcgatg ccgccgacgg caggatgggc gcctgtctcg ccgtcaattt
     2641 catcatcgcc cgcaccacca acccacactg atgacgaagc cggtcgcgcg aggcacgcgc
     2701 ccccccggcg tctactttgc ccggatggtt tcccagggat agccctggtt cagcggctcg
     2761 tgttgtcgca ttaacgaaaa gtcccacggg ccatgtcgac atcggtgaac gattgccgcc
     2821 ggactcagct gacggcctga aaggggttgc ctcatgaatt tcccgatcct ggcccgccac
     2881 tcccgtgtcc atcaccgggt gcgacgtctg cagatgctgg ccgtaacggc ggtcgttctg
     2941 gcgtgcggtg cctcgagcgc ccgggctcag tacacgaacc tgtacggcaa cgccggcacg
     3001 gacggaggcg gaacccaaac ccagcagcac ccggatgatc ccaacgcaac cctgagcggg
     3061 ctgatgtcgc aggacgcggt ggggaacgag gcgtcgggga acgggatgct ctccgcgtgg
     3121 gatggcaagt tccgggtctc cgcgcacgcc tgttcgggtg gcggtccggg ctcgggcggt
     3181 acggccaacg gccaggccac ctgctacgtg taccaggact atcaagtcgt ctcctcgacg
     3241 ctcccggccg gtacaccggt catgatccgg atcaactggg ccgtgagcgg gaaggaaacc
     3301 ggggccggta ccgtgacgca ctatttctac ggcgcggtcg gaggggtgag tggccagatc
     3361 gtcatcaccg tcaacggcgt cagcatcctc aatgtctccg gggaacgccg acgccatatc
     3421 acgtcggagg ggttcagcca ctacacaacc ggaaccctca atgtggaaga ggacagcctg
     3481 acgcacttcg tgcccgtgcc ggtcggtcag gtcgtcagga tcttcatgac ggggacggca
     3541 gccggaagtt ccacggctgg cgcgagctcg gtcgtggacg gcgattcgca acttgccatg
     3601 ctgtggggca ttgcctctct cgatccgaac gcgtcggtga tctcgctcac caacgtgggc
     3661 cacaccccgc cccctcccca gaatgccacg ctgccgaacg ctgtcgacta tcgggccccg
     3721 cgccccgtgg gcctgctgga gtgcttcacg ttctccgagc agccggtcga ggccatcgcc
     3781 tgcgcttcgg gagatgccgg cttcgcggtt gatgtgtcgg gcaccggccc gttcacctac
     3841 cagtggtaca agaatggcgt ggcactgaac ggcctcgcca acccctcggc cacgaccgcc
     3901 tcgctcatgc tgccggacgt caccgccggc gatgcgggtt cctacaccgt caagatcacc
     3961 aatccctgcg gcgatcgcac gagcgacgcg gcgatgctga cggtgactcc ccctccgaca
     4021 atctcgaccc accctgcctc gacatccgtc agtccgccaa ccatggccca agtctcggtc
     4081 gtcgccaacg ggcccgtgga cgactaccaa tggcagatcg agacggcgcc cggcgtgtgg
     4141 gccgacctga gcagcaagtc actcgcactt ccgtgcgggg gcgcggcact gacggatac
//
LOCUS       DASVOD010000615         7142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_403892, whole genome shotgun sequence.
ACCESSION   DASVOD010000615 DASVOD010000000
VERSION     DASVOD010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403892"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VF720_13425"
     CDS             complement(<1..400)
                     /locus_tag="VF720_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880410.1"
                     /translation="MKRLMILALPVAAIVALLLFRPKHDTRVANPVDAGPAVTVAIFR
                     PTAGTRGSSLVLPGRLKAQREISLSSPLASRLTGLPLREGEAFRAGEPLALFDSPEAR
                     QNMESARAAVQAALADLGQARTQSDRIDSLA"
     gene            660..1277
                     /locus_tag="VF720_13430"
     CDS             660..1277
                     /locus_tag="VF720_13430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7880411.1"
                     /translation="MDRLVTVLARPVYQFGRTFCRNPEDAQDVMQEVLSAAVRSVSTA
                     ALRSSLTTWAYTVARNACSRMRRRRVGQPAAFEPLDSAPAGPSDPSRDAESVEIREAL
                     RLALVGLPAAHREAVLLCDVEEVSTAEAAAILGINERALKSRLHRGRAALRARLGERF
                     RPARPRPAAPCTEEMRIQSLCRDAGRQRVPARIKRAVRRALVEQL"
     gene            complement(1274..3355)
                     /locus_tag="VF720_13435"
     CDS             complement(1274..3355)
                     /locus_tag="VF720_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX7880412.1"
                     /translation="MATVLKFKVESDVELKPLAVPKDRPDVKTQLTWYRHMHMATIID
                     DRAKFYIKKAQGWSYHAPVIGHGGIQVALGLSFRQGQDHLFPYYRDLLTCLAGGLSPY
                     EIVLNGLSRRDDVASGGRHMSNHFAKHDLNIWNVSSCTGNHTLHGVGLARANKYYGTD
                     SIVYTSQGESSCSEGYVFEAVNGASREKLPVVFVIQNNGYGISVPVSEQTANPVVSDN
                     FRGFKNVTIINCDGTNPFDSMNAMKKAAAVIKAGTGPVIVHAQCVRYFAHSNSDRQEL
                     YREVEELAEVRSFYPLARMRKHLLETERVPVEVIESLEADNITELDDACVRAEAAATP
                     DPATATDFVYPAEVMPPKAALEPAYTDRDITLREAINETLHEQFQKNPDTFLWGQDVA
                     SKEKGGVFLVTKGMQQAFGEKRVFNAPIAEDFIVGTANGFSRYGDKVHVVIEAAQFAD
                     YLWPSVEQVVELSHDFYRSNGRFAPNVLVRLASGGYIGGGLYHSQNLEAMLMTLPGLR
                     VVAPAFPDDAIGMLRHAMANRGPVFFLEPKYLYNQTFCKTKHPGPDFTVPFGKARLRR
                     EGTDMSMISYGTTVHWCLRAARKLSEEHGIECDVLDLRSIVPMDHDAIIASVRKTGRA
                     LIVHEDKVTGGIGGEIAARISDMAFEYLDAPIRRVGSKDCPVPFSRILEDAVLPAEDD
                     VYAAGLELAKY"
     gene            complement(3511..4470)
                     /locus_tag="VF720_13440"
     CDS             complement(3511..4470)
                     /locus_tag="VF720_13440"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX7880413.1"
                     /translation="MRNPFRELHELMLKGRAGRFMMFGALLVVTDSAFYKRCGCDKET
                     GEIMVGGPLIIAGRTYDYETGAGLPGVQLTFDGAVNDYSVTTGVDGAFSLSDVASGDY
                     QVKTWLDDFDERTKDLTLTTSTTAFDIPLARIHHDALDIEDTWVSSDEPSSNYSTMPY
                     FLIGRKSGPGSQDQAFLTIELPEGAPMDANIHMARMVGFAYSSLSTPMFDPDSPMMLC
                     RVVDPFDPATLTWSNRPDVTDQALGSRLSIEGSDPWRMTFDVKPVLEAYRSTNPSTRS
                     FGVMWVHESLALAKIASTECADAECHGALSGTVDWSGNPPDFP"
     gene            complement(4472..5122)
                     /locus_tag="VF720_13445"
     CDS             complement(4472..5122)
                     /locus_tag="VF720_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880414.1"
                     /translation="MNRSVPRFARSLSLVTATLLLGLAASLPARADDMHDGDGLECGP
                     GRDTVYPGFRVAGSFGYTDDVSSGAPSGFIGVDVGIHKEVLVPGLSVGAVVGWYGLGD
                     ADIAMGDVIVQAEYSLIPAWGQVTYDFMHTDDMLFQGSAGAGAYNKRISEVADCDCTD
                     FGINLGFGVLTRTRRSTNFGAEIRYHHLPREDNSGNKYLTLMARLTFDFGGYGRGM"
     gene            complement(5283..>7142)
                     /locus_tag="VF720_13450"
     CDS             complement(5283..>7142)
                     /locus_tag="VF720_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012240341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX7880415.1"
                     /translation="GQLAVYEEIPKDLLEHVEDVLLNRRPDATERLLAFAETVKGGEK
                     AAVREDAWRKGTVEERLSHALVKGIVEFIDADVEEARLKYARPLQVIEGPLMAGMDVV
                     GDLFGSGKMFLPQVVKSARVMKKAVAQLLPYMEEEKAASGGLGAQGTIVLATVKGDVH
                     DIGKNIVGVVLSCNNWNVIDLGVMVPCENILASAREHDAALIGLSGLITPSLEEMTHV
                     AREMEREGFTTPLLIGGATTSRPHTAIKIAPAYSKATVHVLDASRAVGVATSLTSPEQ
                     RDAFVAENAAIQDEARRTHARKQERVTLLSIGEARARALRTDWATVDIPVPAFTGVRR
                     VDDVSVADLIPWIDWTPFFITWEMGGRYPQILDHPQMGEKARELYADAQAMLERIVTE
                     RWITPRGVYGLFPAETIGDDIVVTPPGGAPVRLHTLRQQAEKDADQPNLALADFVAPA
                     GSRRQDHIGAFAVTSGHGVAERVAAFKKDHDDFNAILLEALADRLAEAFAEWLHARVR
                     REWGYGAGENLTPEQILREQYRGIRPAPGYPAQPDHREKRALFSLLDAEVATGITLTE
                     SLAMSPASSVSGFYYAHPASRYFAVGPVADDQLADYAARTGATPDEIRRWLGL"
BASE COUNT         1142 a         2314 c         2473 g         1213 t
ORIGIN      
        1 gcgccaggga atcgatgcgg tccgactggg tcctggcctg ccccagatcg gccagggccg
       61 cctgaaccgc cgccctggcc gactccatgt tctggcgggc ttccggggag tcgaacagcg
      121 ccaggggctc accggcccgg aacgcctccc cctcccggag tggaagcccg gtcaaccggg
      181 aggccagcgg tgatgaaagg gaaatctccc gctgggcctt caaacgcccg ggaaggacca
      241 ggctggaccc gcgtgtaccc gccgtggggc gaaagatcgc aaccgtcacg gcggggcccg
      301 catccaccgg gttcgcgacg cgcgtgtcgt gcttcggacg gaagagcagc agcgcgacga
      361 tcgcggcgac gggcagggca aggatcatca atcgcttcat caatactccg ttgtgtgctg
      421 gcggccggat gaatagagtc cccacacgtg ggctcatggg gtgtgagcca gtttttccgt
      481 ctgggattca cctcgataac cgatggtgag gaaacaagat agccagcacg aatcggacac
      541 ggaaaagacg gacgccaggg gtcggcagtt cggccacgaa ggcgggccgg ccggcgaacc
      601 tgagccggga gaagcaggac gaactgctgg aggctgcccg cgagggaaat cccgcggcgc
      661 tggatcggct cgtcacggtg ctggcccggc cggtctacca gttcggtcga acgttctgcc
      721 ggaatccgga ggatgcgcag gacgtgatgc aggaggtcct gtcggcggcc gtccgctcgg
      781 tgagcacggc ggcgcttcgc tcgtcactca ccacgtgggc ttacacggtg gcgcggaatg
      841 cctgctcccg catgcggcga cgacgcgtgg ggcaacccgc cgccttcgag ccgctcgatt
      901 ccgcgccggc cggcccttcc gatccctcgc gtgacgcgga aagcgtggaa attcgcgagg
      961 ccctcaggct ggccctcgtc ggcctgcccg ccgcgcatcg ggaagcggtg ctcctctgcg
     1021 acgtggagga agtttcgacg gcggaggccg ccgccattct cggcatcaac gagcgggcgc
     1081 tcaaatcgcg ccttcaccgg ggacgcgcgg cgctgagagc caggctcggc gaacggttcc
     1141 gacccgcccg tccccgcccc gcggcgccat gcaccgagga gatgcggatc cagtcgctct
     1201 gccgtgacgc cggccgccag cgggtgccgg ccaggatcaa acgggcagtc cgacgggcac
     1261 tggtcgaaca gctctagtac ttcgccagct ccagccccgc cgcgtacacg tcgtcctccg
     1321 ccggaagcac ggcgtcctcc aggatgcgcg agaacggcac cgggcagtcc ttgctgccga
     1381 cgcggcggat cggtgcgtcg aggtactcga aggccatgtc ggaaatgcgg gcggcgattt
     1441 cgccgccgat gccgccggtg accttgtctt cgtggacgat cagcgcccgg ccggtcttcc
     1501 tgacggaggc gatgatggcg tcgtggtcca tcggcacgat gctgcgcagg tcgagcacgt
     1561 cgcactcgat gccgtgctct tcggagagct tgcgggcggc gcgcaggcac cagtgcaccg
     1621 tggtgccgta gctgatcatc gacatgtcgg tgccttcgcg gcggagccga gccttgccga
     1681 acggcaccgt gaagtcgggg ccggggtgct tcgtcttgca gaaggtctgg ttgtagaggt
     1741 acttgggctc gaggaagaag acggggccgc ggttggccat ggcgtgacgg agcatgccga
     1801 tcgcgtcgtc cgggaaggcc ggtgccacga cccgcaggcc cggcagggtc atgagcatgg
     1861 cctcgaggtt ctgggagtga tagaggccgc cgccgatgta gccgccggag gcgaggcgca
     1921 ccaggacgtt cggcgcgaac cgcccgttgc tgcgatagaa gtcgtggctc agctccacga
     1981 cctgctccac gctgggccac aggtagtcgg cgaactgggc cgcctcgatg accacgtgca
     2041 ccttgtcgcc gtagcggctg aagccgttgg cggtgccgac gatgaagtcc tcggcgatcg
     2101 gggcgttgaa gacgcgcttc tccccgaagg cctgctgcat tcccttggtg acgaggaaga
     2161 cgccaccctt ctccttgctg gccacgtcct ggccccagag gaaggtgtcc gggttcttct
     2221 ggaactgctc gtgcagcgtc tcgttgatgg cctcgcgcag cgtgatgtcg cgatcggtgt
     2281 aggccggctc gagggcggcc tttggcggca tcacctcggc ggggtacacg aagtcggtgg
     2341 cggtggccgg atcgggagtg gcggccgcct cggcgcgcac gcaggcgtcg tcgagctcgg
     2401 tgatgttgtc ggcttcgagc gactcgatca cttcgaccgg cacgcgctcc gtctcgagga
     2461 gatgcttgcg catgcgcgcg agcggataga acgaacggac ttcggccagc tcctccacct
     2521 cacgatagag ctcctggcgg tccgagttgg agtgcgcaaa gtagcgcacg cactgggcat
     2581 gcacgatgac cgggcccgtg ccggccttga tcaccgcggc ggccttcttc atggcgttca
     2641 tcgagtcgaa cgggttcgta ccgtcgcagt tgatgatcgt gacgttcttg aagccgcgga
     2701 aattgtcgga gacgaccggg ttggccgtct gctcgctgac cggcaccgag atgccgtaac
     2761 cgttgttctg gatgacgaag accaccggca gcttctcccg gctggcgccg ttgaccgcct
     2821 cgaacacgta cccctcgctg caggacgact cgccctggct ggtgtacacg atgctgtcgg
     2881 tgccgtaata cttgttggcc cgcgccagcc ccacgccgtg cagcgtgtgg ttgccggtgc
     2941 agctcgacac gttccagatg ttcaggtcgt gcttggcgaa gtgattgctc atgtggcggc
     3001 cgcccgaggc cacgtcgtcg cggcgtgaca ggccgttgag cacgatctcg tagggggaaa
     3061 ggccccccgc caggcaggtg aggaggtccc ggtagtacgg gaagaggtga tcctgcccct
     3121 gccggaacga caggccgagt gccacctgga tgccgccgtg cccgatgacc ggggcgtggt
     3181 acgaccaccc ctgggccttc ttgatgtaga acttggcccg atcgtcgatg atcgtggcca
     3241 tgtgcatgtg gcggtaccag gtgagctggg tcttgacgtc gggccggtcc ttgggcacgg
     3301 ccagcggttt caattcgacg tcggattcga ccttaaactt aagcactgtc gccatttata
     3361 tcactccggg gtcatgcgcc gagggggcat caaggtccag gcggtcggat taacctatga
     3421 ccttattata gacctgtccg ggcttgcaag cggttacatg gcgccagccg gcaccccccg
     3481 ggccctcgcc cggggagcgc ggcgtcaatc tcaggggaag tccgggggat tgccggacca
     3541 gtcgacggtg ccgctgagcg ccccgtggca ctcggcgtcg gcgcactcgg tcgaggcgat
     3601 cttggcgagg gccaatgact cgtgaaccca catgacaccg aacgaccggg tggacgggtt
     3661 ggtgctccga taggcttcga gcaccggctt cacgtcgaac gtcatgcgcc atggatcgga
     3721 tccctcgatg ctgagccggg atccgagcgc ctggtccgtc acgtcgggcc ggttcgacca
     3781 ggtgagcgtg gccggatcga acggatcgac cacgcggcag agcatcattg gcgaatccgg
     3841 atcgaacatc ggcgtggaaa ggctgctgta ggcgaacccg accatgcggg ccatgtggat
     3901 gttggcatcc atcggggccc cctcggggag ctcgatcgtg aggaatgcct ggtcctgcga
     3961 gcccggcccc gacttccgac cgatgaggaa gtagggcatc gtggaatagt tgctgctcgg
     4021 ttcatcgctc gaaacccacg tgtcctcgat gtcgagggca tcgtgatgga tgcgcgcgag
     4081 cgggatatcg aacgccgttg tcgaggtcgt gagcgtgaga tccttggtgc gctcgtcgaa
     4141 gtcatcgagc caggtcttca cctggtagtc gccggaggcc acgtcggaca ggctgaaggc
     4201 cccgtccacg ccggtggtca cgctgtagtc gttcacggcg ccgtcgaacg tgagctgcac
     4261 gcccgggagg cccgcgccgg tctcgtagtc gtaggtacgc ccggcgatga tgagcgggcc
     4321 gcccaccatg atctcgccgg tttccttgtc gcagccgcag cgcttgtaga aggccgaatc
     4381 ggtgacgacg agcagcgcgc cgaacatcat gaatcgcccg gcacgcccct tcagcatcag
     4441 ttcatgcagc tcacggaatg gattcctcac gtcacatccc ccttccgtag ccgccgaaat
     4501 cgaacgtcag gcgggccatc aacgtcaggt acttgtttcc gctgttgtcc tcgcgcggaa
     4561 ggtggtgata gcggatctcg gccccgaaat tcgtgctcct gcgcgtgcgc gtgagcaccc
     4621 cgaacccgag gttgatcccg aagtcggtgc agtcgcagtc cgccacctcg ctgatccgct
     4681 tgttgtaggc gccggctccc gcggacccct ggaagagcat gtcgtcggta tgcatgaagt
     4741 cgtacgtcac ctgcccccac gcggggatca gcgaatactc cgcctggacg atcacgtctc
     4801 ccattgcgat atccgcgtcg ccgaggccgt accatcccac cacagcgccc acgctgaggc
     4861 cggggaccag cacttccttg tggatgccga catccacgcc gatgaagccg ctcggggccc
     4921 ccgaggagac gtcgtcggtg tagccgaagc tgccggcgac gcggaagccc gggtacaccg
     4981 tgtcgcgtcc ggggccgcac tcgaggccgt cgccgtcgtg catgtcgtcg gcgcgggccg
     5041 ggagggatgc ggccaggccc aggaggagcg tggcggtcac aagggaaagg gaccgtgcga
     5101 agcggggcac ggatcggttc atgtcggggt gtcctccctg gcatgagagt catgggcgga
     5161 caacacgaag ccttcgacga ttgccgttgc cgcccttatt cattcgaacg caatgcgaag
     5221 gctgtaaccg ccggggaaca tagcacatgg ccccggggag aagggaaatg gccggtccgg
     5281 agctacaggc ccagccaccg cctgatttca tccggcgtag ccccggtccg ggcggcgtag
     5341 tcggcaagct ggtcgtccgc cacggggccc acggcaaagt agcgactggc gggatgggcg
     5401 tagtagaagc cgctcaccga actggcgggc gacatggcca gcgattcggt cagcgtgatt
     5461 cccgtcgcca cctcggcgtc gaggagcgaa aagagggcgc gcttctcgcg gtggtcgggc
     5521 tgggccgggt agccgggcgc gggacggatg ccgcgatact gttcgcggag gatctgttcg
     5581 ggagtcaggt tttcgccggc gccgtatccc cattcgcgac gcacgcgggc gtgcagccac
     5641 tcggcaaacg cctcggccag gcggtcggcc agcgcctcga gcaggatggc gttgaaatcg
     5701 tcgtgatcct tcttgaaggc cgccacccgc tccgccacgc cgtgacccga cgtgaccgca
     5761 aaggcgccga tgtgatcctg gcgcctggaa ccggccggcg ccacgaaatc ggccagggcc
     5821 aggttcggct ggtcggcgtc cttttccgcc tgctggcgta gcgtgtggag ccgcaccggc
     5881 gcgccgccgg ggggcgtgac cacgatgtcg tcgccgatcg tttcggccgg gaagaggccg
     5941 tacacgcctc ggggcgtgat ccaccgctcg gtcacgatcc gctcgagcat ggcctgcgca
     6001 tcggcgtaca gctcgcgcgc cttctccccc atctggggat ggtcgaggat ctgcgggtag
     6061 cggcccccca tctcccaggt gatgaagaat ggggtccagt cgatccaggg gatcaggtcg
     6121 gccaccgaga cgtcgtcgac ccgccggaca ccggtgaagg cgggcaccgg gatgtcgacc
     6181 gtggcccagt cggtgcgcag ggcgcgcgcg cgcgcctcgc cgatcgacag gagcgtcacg
     6241 cgttcctgct tgcgcgcgtg cgtgcgccgc gcttcgtcct ggatggcggc gttctcggcc
     6301 acgaacgcgt cacgctgttc ggggctggtg agcgaggtgg ccactcccac cgcgcggctg
     6361 gcgtccagca cgtgcacggt ggccttcgag taggccgggg cgatcttgat ggccgtgtgg
     6421 ggacggctgg tggtggcgcc accgatgagc agcggcgtgg tgaagccctc gcgctccatt
     6481 tcgcgcgcca cgtgcgtcat ctcctcgagc gacggcgtga tgaggcccga caggccgatc
     6541 agcgcggcgt cgtgctcgcg ggccgatgcc aggatattct cgcacggcac catgaccccg
     6601 agatcgatga cattccagtt gttgcacgac agcaccacgc ccacgatgtt cttgccgatg
     6661 tcgtgcacat cccccttcac ggtggccagc acgatggtgc cctgcgcgcc caggccgccg
     6721 gaggccgcct tttcctcttc catatacggc aggagctgcg ccaccgcctt cttcatgacg
     6781 cgcgcgctct tcacgacctg gggcagaaac atcttgccgg aaccgaacag gtcgccgacg
     6841 acgtccatgc cggccatcag cggcccttcg atcacctgca gcgggcgcgc gtacttgagg
     6901 cgcgcctctt cgacatcggc gtcgatgaac tccacgatcc ccttgacgag ggcgtgcgac
     6961 agccgctcct cgacggtgcc cttgcgccag gcgtcctcgc ggaccgcggc cttctcgcca
     7021 cccttcaccg tctcggcgaa ggcgagcagg cgttcggtgg catcggggcg gcggttcagg
     7081 agaacgtctt ccacgtgctc gaggaggtcc ttcgggattt cctcgtacac ggccagctgg
     7141 cc
//
LOCUS       DASVOD010000616         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_769481, whole genome shotgun sequence.
ACCESSION   DASVOD010000616 DASVOD010000000
VERSION     DASVOD010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769481"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="VF720_13455"
     CDS             complement(<1..>1501)
                     /locus_tag="VF720_13455"
                     /inference="COORDINATES: protein motif:HMM:NF013506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEX7880416.1"
                     /translation="THDLGVMVRPRRYLSLGLAARNLNDPAFADGRLPRGYATGVSVR
                     PLGNDPERLTTTLQADWRTTGDAARWTIGGEYRVIDGLRLLAHWKSRPDELGLSVAFD
                     LPGTTLRTTSLHRDDDPKRRTSVSLQTHGDAFRRESFPVMKRFAEVRLEGTYGDQASG
                     FVLLGHPTKSLHRVLTNLEKARNDSDVSGVIVRIGDLGGAFIGGVAAVHQELFRALLA
                     VRESGKPVVAYLEEGGGAAELYVAAAADRIVMPRLARFQGIGVSIEILRLKEAFADLG
                     IDWDAVTAGPYKSTFHTWFTEAATPAQKAELESLVAAAMAELDSTLTGARDLSPAARS
                     RLFDGGLVGAPEALTLGVIDRIGWYEDARRVAAELADAGTDPARTRRLDRRRYWRERW
                     TPPPAVAVVLASGAITTGESRVNRLSGSRTMGADTVVRALRSAAAAPDVKAIVLRIDS
                     GGGSALASDQIRAEVERLRNSRGVPILVSMGDAAASGGYWIATNADRILA"
BASE COUNT          222 a          537 c          536 g          206 t
ORIGIN      
        1 gccaggatcc gatcggcgtt ggtggcgatc cagtaaccgc ccgaagccgc ggcatcaccc
       61 atcgacacga ggatcggcac cccccgggaa ttgcgcagcc gctccacttc ggcccggatc
      121 tggtcggagg cgagcgccga gccgccaccc gagtcgatcc gcagcacgat ggccttgacg
      181 tcgggcgccg ccgcggccga tcggagcgcg cgcacgaccg tgtcggctcc catcgtccgc
      241 gaaccagaca ggcgattgac ccggctctcg ccggtcgtga tggcccccga ggccaggacc
      301 acggccacgg ccggcggcgg cgtccatcgt tcgcgccagt agcggcgacg gtcgaggcga
      361 cgggtgcgcg ccggatcggt gccggcgtcg gccagctcgg ccgcgacgcg ccgggcatcc
      421 tcgtaccacc cgatcctgtc gatcacgccc agggtgagcg cctcgggcgc gcccaccagt
      481 cccccgtcga agagccgcga acgcgcggcc ggcgagaggt cgcgcgcgcc ggtcagcgtg
      541 gaatcgagtt cggccatcgc cgccgccacc agcgattcga gctccgcctt ctgggcgggg
      601 gtggcagcct cggtgaacca ggtgtggaac gtcgacttgt agggccccgc ggtcaccgcg
      661 tcccagtcga tgccgagatc ggcgaaggcc tccttcagcc ggaggatctc gatgctgacg
      721 ccgatcccct ggaagcgtgc cagccgcggc atcacgatcc ggtcggcggc tgccgccaca
      781 tagagttcgg ccgccccccc tccctcttcg aggtaggcca ccactggctt gcccgactcg
      841 cgcacggcca gcagcgcccg gaagagttcc tggtgcaccg ccgccacgcc gccgatgaag
      901 gccccgccca gatcgccgat ccggacgatc acgcccgaga cgtcagaatc gttgcgggcc
      961 ttctcgagat tggtcaggac gcgatggagg cttttcgtgg ggtggccaag caggacgaag
     1021 cccgaggcct ggtcgccgta cgtcccctcg agccggactt cggcgaagcg cttcatcacc
     1081 gggaatgatt cgcgacggaa ggcatcgccg tgcgtctgga gcgagaccga ggtgcgccgc
     1141 ttcgggtcgt cgtcccggtg gagcgaggtg gttcggagcg tcgtgccggg aaggtcgaac
     1201 gccaccgaca ggcccagctc gtcgggccgg ctcttccagt gggccaggag ccggaggccg
     1261 tcgatcacac ggtactcgcc gccgatggtc cagcgggcgg catcgcccgt cgtgcgccag
     1321 tcggcctgga gcgtggtggt gaggcgctcg ggatcgttcc ccagcggccg gaccgagacg
     1381 ccggtggcat agccgcgcgg gaggcggccg tcggcgaagg ccggatcgtt caggttgcgt
     1441 gccgccaggc ccagtgacag atagcgccgc ggccgcacca tcacaccgag gtcgtgcgtc
     1501 g
//
LOCUS       DASVOD010000617         7606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_404019, whole genome shotgun sequence.
ACCESSION   DASVOD010000617 DASVOD010000000
VERSION     DASVOD010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7606
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404019"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VF720_13460"
     CDS             complement(<1..849)
                     /locus_tag="VF720_13460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7880417.1"
                     /translation="MRLLHVNKFHWPKGGSERYYFDCVNGLSARGHEVAVFASAHPSN
                     VASPWSKHFAPGADYHGAGPSDALRLAGHVIDNQPAARALEGLLREFRPEVAHLHNIH
                     HQLSPAILAVLERAGVPVVQTLHDYKWVCPAYLFLSHGKVCEKCGPGNDFFPVVTHTC
                     HHDSFPKSLVVWRELTASWGRGDVDRVKRFLAPSRFLRDRVVAHGLPNDRVETRSYFL
                     DVAAYRPADAPSGDHFLYAGRLSKEKGLSTLVAALARRPGLKPRVAGAGPLESELRER
                     ARAENLD"
     gene            complement(860..2170)
                     /locus_tag="VF720_13465"
     CDS             complement(860..2170)
                     /locus_tag="VF720_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015333228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX7880418.1"
                     /translation="MALLAVMALFIPEGKSVAVGGPVDPGDTAWLLTATCLVLLMTPG
                     LSFFYGGMVSRKNVISTMLQSFVALGVVSLIWVAFGFSLAFGDSWHGVIGDPRTFFMF
                     RGVGGDTHPDLAPTIPLVLFAMFQLKFAIITPALITGSFAERVKFSGYLVFMALFTVF
                     VYCPLAHWTWHPQGFLRQWGVLDFAGGTVVHMSAGLAALAGALFLGPRRQHASGHTAP
                     ASNIPYVLLGTGMLWFGWFGFNAGSSLGANAVAAGAVATTNTASAAAMLTWMLFDVAR
                     GRRASALGACIGAVVGLVAITPAAGYVSIGSSVFIGVLAAVVSNWAAHLKSRSTLDDT
                     LDVFPCHGVGGIVGMLATGLLARDVGLMAGKTHTMLFHLLALVIVSAFAFTVSFVLYW
                     LTDRLITLRVGEAQEAIGLDISQHDEFLENDPFRQFGDMPPARD"
     gene            complement(2372..3013)
                     /locus_tag="VF720_13470"
     CDS             complement(2372..3013)
                     /locus_tag="VF720_13470"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX7880419.1"
                     /translation="MSLSLYLLRHGQTDQSRENVFCGAGLDPELTADGRAMAEAFAAA
                     YRTTLWSAIYSSSQRRAMATAAPLATAVGVAPEVRDGLKEIAYGAWEGLSAEQVGREF
                     HDDHIRWTADPAWNAPTGGEPATMIARRAMKVVEEIIGNCRDGNVLVVSHKATIRILL
                     CALLGIEIGHFRFRLACPVGSVSVVEFGDHGPMLKGLADRRHLDERLRNLPGT"
     gene            complement(3023..6382)
                     /locus_tag="VF720_13475"
     CDS             complement(3023..6382)
                     /locus_tag="VF720_13475"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7880420.1"
                     /translation="MIQPKTTHRMVLAHHSRLGQLYVPGINARVPGPAGPYFLKTNAQ
                     GFRSDWDFTKEKGRKPRILVFGDSYTAGDGVTNADRFSEKLGQMYGAEVFNFGVAGTG
                     TDQHLMILEEVARGVKADLVIFCVAVHNIDRIKVGYRKYIERTTGEHVLGAKPYFELI
                     KGKLQLRNVPVPIERPRVDEAGDVAYEQPVVHDKQGGFGLFKKVRQGLGLEKMEKTLD
                     KLAPDVKTTLRTKLLHKQDFQPHEDYRSASSPGWQLMKALIERFHAGVKPTPLLVVPI
                     PTWYFYFDEVPAIYQPLFESLENKPAGIHVMDATSPLLAMPREDRRRLPLGTDTHFSP
                     EGHTKIAEMIASEIDARSLLPGRREMLARSVVGRRRAAEAAALLENGDAPAADANRPR
                     WILGLSCFYHNSAAALIKDGRIVAAAEEERFSRVKNDPRFPVNAINFCLEQGNIQPKD
                     LAAAVYYDNAALTFERIFHTLMALGKDGEDAWMRILPFWVGQKFHIPELVREALGYDG
                     LVLQETHHRSHAASAFYPSPFERAAILTIDGVGEWATATIGVGRGAEMKLLQEMRFPH
                     SLGLLYSAFTQFTGFKVNSGEYKMMGLAPYGEPKYVDRILDKLVTLHDDGSIELNMEY
                     FGFLHEASMTSDKMAELFEGPAREIESRITRREMDMARSVQVVTEEAMIRMARHAHRL
                     TGESKMCLAGGVALNCVANGRVLREGPFEDMWIQPAAGDSGGALGAALDAHHTWFREP
                     RVMPANGRADQQASWWGPGYTNDEIEAFLRTYGIPAVRLAPEERAGRLADLLAEGKIL
                     GHFAGRTEFGPRALGARSILGDPRNPDMQARLNLKIKFRESFRPFAPAVLAEKVGDYF
                     ELDRESPYMLLVAPVRKERRLVPEKVEGEDMLVTVKQLRSDIPAITHIDYSARVQSAT
                     KDETGPYYDIIKAFDQKTGCAVIVNTSFNVRGEPIVNTPAEAYRCFMKTHMDALVLGD
                     FLLLKEDQPATSLVPEPVSKTVNTRELESPLSEELRRIYREEFLPLVTRLDGRGVEVS
                     EKPTGQPTLWRDLHESDDPRAIFQVPERLTNPNAPPAEAAQAMLRYWGEGPVRDAAEP
                     VLARLLALSRKMPLALDQGHAKVHDTIYVMY"
     gene            complement(6421..>7606)
                     /locus_tag="VF720_13480"
     CDS             complement(6421..>7606)
                     /locus_tag="VF720_13480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7880421.1"
                     /translation="RGDCRPKGWADGRPAATMRRMRVAFVASRLALGGAERITGEVSE
                     RLAARGHQVVWLLLRDRGPEGERLAARGLNVEHDLGSWRFDPFTSGRLAGRLRHHRIE
                     SAWCLDHQNAAVNLALALRGAPDVRAFLAIHTTGLWGGGSSLPGGMRAALPAFTNVMA
                     VADAQKRYLIDHLSIPAERVVVIRNGVDVAAFATTAERRANADALRATWGIPSGAAVL
                     GVVAALRPEKGHADLFSILGRESPLGQTHLVLVGEGDERSPLEALAARLAPGRIHFLG
                     ARADVADILPALDVVVLPSKPLVETLPLSVMEAMAAGRPVVATRVGALEELIEDGVDG
                     RLVPPDDNGALSGTLVDLLSNQAERERLGAAAARRATQFDIERTVEAMTDLLGGRFPG
                     LL"
BASE COUNT         1241 a         2596 c         2561 g         1208 t
ORIGIN      
        1 atcaaggttc tccgcgcgcg cccgctcgcg gagttccgat tcgagcggcc ccgcccccgc
       61 cacgcggggc ttgaggccgg gccgacgcgc cagcgcggca acgagcgtcg acagaccctt
      121 ctctttcgag aggcgcccgg catacaggaa gtgatcgccg gacggcgcgt ccgccggtcg
      181 gtatgcggcc acgtccagga agtaggaacg cgtctccacg cgatcattcg gcagcccatg
      241 cgccaccacc cggtcgcgca ggaagcggct cggcgcgagg aaccgcttca cgcggtccac
      301 gtctccccgc ccccacgatg ccgtgagctc gcgccacacg acgaggctct tcggaaagct
      361 gtcgtgatgg caggtatgcg tgacgaccgg aaagaaatcg ttccccggcc cgcacttctc
      421 gcagaccttt ccgtgcgaca ggaacaggta ggccgggcag acccacttgt agtcgtgcag
      481 cgtctggacg accggcaccc ccgcccgctc gagcaccgcg aggatcgccg gtgaaagctg
      541 gtggtggatg ttgtgcaggt gcgccacctc cggccggaac tcgcgcagca gtccttcgag
      601 cgcccgcgcc gcgggctggt tgtcgatgac gtgacccgcc agccggagcg cgtccgacgg
      661 acccgccccg tgataatccg cgcccggcgc gaagtgcttc gaccagggcg aggccacgtt
      721 cgacggatgc gccgacgcga acacggccac ctcgtgcccc cgggccgaga ggccgttcac
      781 gcagtcgaag tagtagcgct cggagccccc cttgggccag tggaacttgt tgacgtggag
      841 gaggcgcatc gcagcgaaat caatcccgtg ccggcggcat gtcgccgaac tggcggaacg
      901 gatcgttctc gaggaactcg tcgtgctggc tgatgtcgag gccgatggct tcctgcgcct
      961 cgccgacgcg cagcgtgatg agtcggtcgg tgagccagta gaggacgaac gatacggtga
     1021 acgcgaaggc gctcacgatg acgagtgcaa ggagatggaa cagcatcgtg tgcgtcttcc
     1081 cggccatgag gcccacgtcg cgcgccagga gccccgtggc cagcatgccg acgatgccgc
     1141 cgacaccgtg gcacgggaag acgtcgagcg tgtcgtccag cgtggagcgg gacttcaggt
     1201 gggcggccca gttgctgacg accgccgcca gcaccccgat gaagacgctg ctcccgatcg
     1261 acacgtagcc ggccgccggc gtgatggcca cgaggccgac caccgcgccg atgcaggccc
     1321 cgagcgccga ggcgcgccgg ccccgggcca cgtcgaacag catccaggtg agcatggccg
     1381 cggccgaggc cgtgttcgtg gtggccaccg ctccggccgc cacggcattg gcgccgagcg
     1441 acgaccccgc gttgaacccg aaccatccga accagagcat gccggtgccc agcagcacgt
     1501 acgggatgtt ggaggccgga gccgtgtggc cgctggcgtg ctggcgacgc ggcccgagga
     1561 agagcgcccc ggccagcgcc gcgaggccgg ccgacatgtg cacgacggtg ccgccggcga
     1621 agtcgagcac cccccactgg cggaggaacc cctgcggatg ccacgtccag tgtgccagcg
     1681 ggcagtaaac gaagaccgtg aagagcgcca tgaagaccag gtatcccgag aacttgaccc
     1741 gctcggcgaa cgaccccgtg atcagggccg gtgtgatgat ggcgaacttg agctggaaca
     1801 tcgcgaagag caccagcggg atcgtcggcg ccaggtcggg atgggtatcg cccccgacgc
     1861 ctcggaacat gaagaaggtg cgcgggtcgc cgatcacccc gtgccacgaa tcgccgaacg
     1921 ccaggctgaa cccgaacgcc acccagatga ggctgacgac gcccagcgcc acgaagctct
     1981 gcagcatcgt cgagatcacg ttcttccggc tgaccatccc gccgtagaag aacgagagtc
     2041 cgggcgtcat gaggaggacg aggcaggtgg cggtcaggag ccacgccgtg tcgcccgggt
     2101 cgaccggacc gccgacggcc accgattttc cttcgggaat gaacagcgcc atcacggcca
     2161 gcagggccat gatgtagagc gggtatcgct tcggccgatg gttcatgggc gggaatctag
     2221 cagattgatc cgtgcgggac ggtgggggcc ggggacatca tctgaatcag gaacagggtc
     2281 gggtactcgg ttcaggcgga gttaaacctg actccaattc aggccggtca actaggttca
     2341 ggcagagttc gacggggacg ccggccccct gctacgtccc cgggaggttc cggagccgct
     2401 cgtccaggtg tcgccggtcg gccagcccct tcagcatcgg cccatggtca ccgaattcga
     2461 cgacgctgac cgatcccacc ggacaggcca gtcggaaacg gaagtgaccg atctcgatgc
     2521 cgaggagcgc gcagagcagg atccggatcg tggccttgtg ggagacgacg aggacatttc
     2581 cgtcgcggca gtttccgata atctcctcga ccaccttcat ggcccgccgg gcgatcatcg
     2641 tggccggttc gccaccggtc ggggcgttcc aggcagggtc cgcggtccag cggatgtggt
     2701 cgtcgtggaa ttcccggccg acctgttccg cactcaggcc ttcccaggcg ccgtaagcga
     2761 tctccttcag gccatcgcgc acttcgggcg ccacaccgac cgcggtcgca agcggcgccg
     2821 cggtggccat cgcccgtcgc tgggagctcg agtagatggc ggaccacagc gtggtgcggt
     2881 aggcggcggc aaaggcctcg gccatggccc ggccatcggc cgtcagctcc ggatcgagac
     2941 cggcgccgca gaaaacgttc tcgcggctct ggtcggtctg gccgtgccgg aggaggtaca
     3001 gggagaggga catgccccga gcctagtaca tcacgtagat cgtgtcgtga accttggcgt
     3061 gcccctggtc gagcgccagc ggcatcttcc ggctcagcgc cagcaaccgc gccagcaccg
     3121 gctcggcggc gtcacggacg ggcccctcgc cccagtagcg gagcatggcc tgggccgcct
     3181 cggccggcgg tgcgttcggg ttggtcagac gctcgggcac ctggaaaatg gcgcgcgggt
     3241 catccgattc gtggaggtcg cgccagagtg tgggctggcc ggtgggcttc tccgacacct
     3301 ccacgcccct tccatcgagg cgcgtgacca gcggcaggaa ctcctcccgg taaatcctgc
     3361 ggagttcctc ggaaagtggc gactccagct cgcgcgtgtt cactgtcttc gacacaggct
     3421 ccggcaccaa cgacgtggcc ggctgatcct ctttcaacaa caggaaatcg cccagcacca
     3481 gcgcatccat gtgcgtcttc atgaaacagc ggtacgcctc ggccggcgtg ttcacgatgg
     3541 gctcgccgcg cacgttgaaa ctggtgttca cgatcacggc gcagccggtc ttctggtcga
     3601 aggccttgat gatgtcgtag tacggccccg tctcgtcctt cgtggccgac tggacgcgcg
     3661 ccgagtagtc gatatgcgtg atggccggga tgtcgctccg gagctgcttg acggtgacca
     3721 gcatgtcctc cccctccacc ttctcgggga cgaggcggcg ctccttgcgg acgggcgcca
     3781 cgaggagcat gtacgggctc tcgcggtcga gctcgaagta gtcccccacc ttctcggcca
     3841 ggacggccgg cgcgaagggg cggaacgact cgcggaactt gattttcagg ttgagacgcg
     3901 cctgcatgtc ggggttgcgc ggatcaccca ggatgctgcg ggcgccgagg gcgcgcgggc
     3961 caaactcggt gcgtccggcg aaatggccga ggatcttccc ctcggccagc aggtcggcca
     4021 gtcggccggc ccgttcctcg ggtgcgagac gcaccgccgg tatcccgtac gtgcggagga
     4081 acgcctcgat ctcgtcgttc gtgtagcccg gcccccacca cgaggcctgc tgatcggcgc
     4141 ggccgtttgc gggcatcacg cgcggctccc tgaaccaggt gtgatgcgcg tcgagcgcgg
     4201 cgccgagcgc gccgcccgaa tcgcccgccg cgggctggat ccacatgtcc tcgaacggcc
     4261 cctcgcgcag gacgcggccg ttggccacgc agttgagggc cacgccgccg gccaggcaca
     4321 tcttcgattc gccggtcagc ctgtgggcgt ggcgggccat ccggatcatg gcttcctccg
     4381 tcaccacctg gaccgagcgg gccatgtcca tctcgcggcg cgtgatgcgg ctctcgatct
     4441 cgcgcgccgg gccctcgaaa agctcggcca tcttgtcgct cgtcatcgaa gcttcgtgca
     4501 ggaagccgaa gtactccatg ttgagctcga tcgagccatc gtcgtggagc gtgaccagct
     4561 tgtcgaggat cctgtcgacg tatttcggtt cgccgtacgg cgccagcccc atcatcttgt
     4621 actcgcccga gttcaccttg aagccggtga actgcgtgaa ggccgagtag aggaggccga
     4681 gcgagtgcgg gaagcgcatc tcctggagga gcttcatttc cgcgccccgc cccacaccga
     4741 tcgtggcggt ggcccactca cccacgccgt cgatcgtgag gatggccgcc cgttcgaagg
     4801 gggatgggta gaacgcgctg gccgcatggc tgcggtggtg ggtttcctgc agcacgaggc
     4861 cgtcgtaacc gagcgcctcg cgcaccagtt cggggatgtg gaacttctgc cccacccaga
     4921 acggcaggat gcgcatccag gcgtcctcgc catcctttcc gagcgccatc agcgtatgga
     4981 agatccgctc gaacgtgagc gcggcattgt cgtagtacac cgcggccgcg agatccttcg
     5041 gctggatgtt cccctgttcc aggcagaagt tgatggcgtt gaccgggaag cgcggatcgt
     5101 tcttcacgcg gctgaagcgt tcttcctcgg cggcggccac gatccttccg tccttgatca
     5161 gcgccgccgc ggagttgtga tagaagcagg agaggccgag gatccagcgc ggcctgttcg
     5221 cgtcggcggc cggcgcgtcg ccgttttcga ggagggccgc ggcctccgcg gcccggcggc
     5281 gccccaccac gctacgggcc agcatctcgc gccgtcccgg cagcagcgag cgcgcatcga
     5341 tctcgctcgc gatcatttcc gcgatcttcg tgtgcccctc cggcgagaag tgcgtgtcgg
     5401 tgccgagcgg caggcgtcgg cgatcctcgc gcggcatggc caggaggggc gaggtggcgt
     5461 ccatcacgtg gatgccggcc ggcttgttct ccagcgattc gaagaggggt tggtagatgg
     5521 ccggcacttc gtcgaaatag aagtaccagg tggggatcgg cacgacgagg agcggcgtgg
     5581 gcttgactcc ggcgtggaag cgctcgatga gtgccttcat cagctgccag cccggcgaag
     5641 aggccgagcg gtagtcctcg tgcggctgga aatcctgttt gtggagaagc ttggtccgga
     5701 gcgtggtctt gacgtccggc gccagcttgt cgagcgtctt ctccatcttc tcgaggccga
     5761 gcccctgccg caccttcttg aagaggccga agcccccctg cttgtcgtgc accaccggct
     5821 gctcgtaggc cacgtccccc gcctcgtcga ccctggggcg ctcgatcggc accgggacgt
     5881 tccggagctg gagctttccc ttgatcaatt cgaagtaggg cttggctccc agcacatgct
     5941 cgccggtcgt tcgctcaatg tatttccggt accccacctt gatccggtcg atgttgtgaa
     6001 cggccacgca gaaaatgacg agatcggctt tgacccccct ggccacttcc tcgaggatca
     6061 tcaggtgctg gtccgtgccg gtgccggcca cgccgaagtt gaagacctcg gcgccataca
     6121 tctgccccag cttctccgaa aaccggtcgg cgttggtcac gccgtctccg gccgtgtagg
     6181 aatctccgaa gacgaggatc ctcggctttc tccccttttc cttcgtgaaa tcccagtcgg
     6241 agcggaatcc ctgggcgttg gtcttcagga aataggggcc ggccggcccg ggaacgcggg
     6301 cgttgatccc cggcacgtag agctggccga ggcggctatg gtgggccagg accatccggt
     6361 gggtggtctt gggctggatc atgcgtcagt ctctccgagg gtggccaaag gaacgcacga
     6421 ttatagcaag cccgggaacc ggcctccgag caggtcggtc atggcctcga ccgtgcgctc
     6481 gatgtcgaac tgcgtggccc tgcgggccgc cgcggcaccc agccgttccc gctcggcctg
     6541 attcgacagc aggtcgacaa gcgtgccgga tagcgccccg ttgtcgtcgg ggggcaccag
     6601 ccgtccgtcg accccgtcct cgatcagctc ctccagcgct ccaacccggg tggccacgac
     6661 cggccgaccg gccgccatcg cctccatgac cgacagggga agcgtttcca ccagcggttt
     6721 cgacgggagc accaccacgt cgagggccgg caatatatcg gccacatccg cccgcgcccc
     6781 gaggaaatga atccggccgg gggccagccg cgccgccagg gcctccagag gggagcgctc
     6841 gtccccctcg cccacgagaa cgaggtgggt ctgccccagg ggactctcgc gacccagtat
     6901 tgaaaagagg tccgcgtgcc ccttctccgg ccgcagcgcc gccacgacgc cgagcaccgc
     6961 ggccccggag gggattcccc atgtcgcgcg cagcgcgtcc gcgttggccc gccgttcggc
     7021 cgtcgtggcg aacgcggcca catcgacgcc gtttcggatc accaccaccc gctccgcggg
     7081 gatggaaagg tggtcgatca ggtaacgctt ctgggcgtcg gccaccgcca tgacgttcgt
     7141 gaacgcgggc aacgccgcgc gcatcccgcc cggaaggctc gagccgcccc cccataaccc
     7201 ggtggtgtgg atggcgagga acgcgcggac atccggggcg ccccgcaacg ccagcgccag
     7261 gttcaccgcg gcgttctggt ggtcgaggca ccaggccgat tcgatccggt gatggcgcaa
     7321 ccggccggcc aatcgcccgg aggtgaaagg atcaaaccgc cacgatccga gatcgtgttc
     7381 gacattcagg ccccgcgccg ccaaccgctc cccttcgggc ccgcggtcgc ggaggaggag
     7441 ccagacaacc tggtgcccac gggccgccag ccgctccgac acctcccccg tgattctctc
     7501 ggccccgccg agtgccagcc tcgaggccac gaacgccaca cgcatgcggc gcatggtagc
     7561 cgctggccgc ccgtccgccc accccttggg ccggcaatcc cccctt
//
LOCUS       DASVOD010000618         5209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_926301, whole genome shotgun sequence.
ACCESSION   DASVOD010000618 DASVOD010000000
VERSION     DASVOD010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5209
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926301"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF720_13485"
     CDS             <1..228
                     /locus_tag="VF720_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880422.1"
                     /translation="EPDRFSTPAWIMAAGHPGHAGSMSSRLDSITANLAGLEANLSAL
                     ESALSTSTSSGGGFSGGGAGGASGGGASGAS"
     gene            501..3005
                     /locus_tag="VF720_13490"
     CDS             501..3005
                     /locus_tag="VF720_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7880423.1"
                     /translation="MTHHLTLPGRARRAAVSILAILVLPVIAFARPVQSPGLSAFNAA
                     EPDRPGFVVNNLPGSGLVIDLMVEGPATDSELEALGAIIGSRLPNGVRTVRMPVARLD
                     DLRALPSLTRLVASRPVRMLNDSSVPLTLATPNYWTSAPPNFTGSAGTNVIVGIVDSG
                     IDWSHDDFKNPDGTTRILNIWDQNIAGVNPAGFAYGNECTQAQINAGTCSETDAVGHG
                     THVAGSAAGDGSATGNGQPANRFIGMAPRANIIVVATNFSGNGVVDGVNYIFQKAAAL
                     GRPAVVNLSLGSQYGAHDGTEGLDTALSALTGPGKIIVASAGNEGGSSRHAEQLVPPG
                     PAQTITFNVPAYAAAVGANNDYVILDCYYNGSANMSVTVTSPGPTPVVLGPITKGTNG
                     GSGASLAGTIYAENGFTPSASGATNILIQVFDSNSARTPRVGVWTVTLTPVATVASTE
                     CDLWLADFHLGTAGVAATFTSDVENAELVGTPGTGNSIISVAAFTSKQFWPSIDTNSY
                     SFVGSTPAGPIAGFSSPGPRRDGAQKPDIAAPGTAIVSSRSTATAGIPNALINPDGVH
                     WTQQGTSMSSPHVTGAVALYLADSPTMTPAQARTRLAADALVDGNTGAVPNATWGAGK
                     LRMIRADAVPPMVTLTAPNGGQVFIEGSMQNITWTATDNVGVTAIDLAYSTNNGGAWT
                     TIVNGEPNDGTYSWTVPAVTTIQALVRVTAHDALNTASDASNAVFAITSLSAAPDRSA
                     PPVRPIALANAPNPFGASTIITFGLPANENVKLSIVSPAGRLVRRLLDGSFPAGWHDT
                     AWNGTADDGRQVAQGIYFYKFEAGDVVTTQRMVLTR"
     gene            complement(3013..4875)
                     /locus_tag="VF720_13495"
     CDS             complement(3013..4875)
                     /locus_tag="VF720_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880424.1"
                     /translation="MNPEAHPEEEFTGKAFDRRLMGRLLGYLGPHRGAVILATILLLA
                     TSALELAGPWLTQQAIDVAIPAHDNRHLLVLTVAFFGALAGALLLTWFQTWLMQRVGQ
                     LVMYEMRKQIFRHQLGQSSAWFDRNPVGRMLTRLTTDVDVLNEMLTSGVVALFGDLVT
                     LFGILGILFWLDWRLTLATLIVMPLLVIVTAWFRRGVRETYRMVRTRIARINTFLQEN
                     ITGMSVVQIFGREAHRNRHFDRLNADHLDAHIRSIFYYAVFYPLIEVISSVALALVLW
                     YGGLRTMGDAGGASLLSLGVLVAFIQYVRRFYRPIMDLSEKYNILQAAMASAERIFRM
                     LDAPQVIPVPDAPAPWSGFSSSIEFRNVWFAYNDEEWVLRDVSFTVRHGETVALVGAT
                     GSGKTTIISLFCRFYDIQKGSILVDGHDIRTLDVSALRRSIGLVLQDVFLFRGTIADN
                     IRLGDRDIDDGRVADAIRHVNAGRFIDTLPDGLGARLGERGASLSTGQKQLLAFARAL
                     AHRPEVLVLDEATSSVDTETEQLIQEALHRLLSGRTSIVIAHRLSTIQDADRILVLHK
                     GQLREQGSHRELLAHGGLYFKLYQLQYKDQEITTARPAGEGPGRAVAGVPPARS"
     gene            complement(4892..>5209)
                     /locus_tag="VF720_13500"
     CDS             complement(4892..>5209)
                     /locus_tag="VF720_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880425.1"
                     /translation="QKQRTAIARAILRRPRILVLDDALSSVDTKTEEDILTRLREPMA
                     TRTTLIVSHRVSTVRHADRIVVLEAGRIVESGTHDELLTLGGRYADLERRQRLREEIE
                     ATA"
BASE COUNT          875 a         1822 c         1724 g          788 t
ORIGIN      
        1 gagccggatc gtttttccac gcccgcctgg atcatggcgg ccggccaccc cggacatgcc
       61 gggtcgatga gctcccggct cgattcgatc acggccaacc tcgccggcct cgaggccaac
      121 ctctcggctc tcgagagcgc cctctccacg agcacgtcgt cgggcggcgg cttttcgggg
      181 ggcggcgcgg gcggcgcctc gggcggtggg gcgagcggag cgagctgacg ccggcacgcc
      241 ggtaatttcg caccggctgt ctcaggttgc gtcaccatca caatccgatc gaaccaaacg
      301 gcgttgcgcg cgttctaaag cacgcttcgg gtgagttcac ccggttcgat cgcggaattt
      361 ttcgcagtgc gccttgcacc acatcgagcg tcattccaaa tttcgtcagc cggaatcccc
      421 attcggccga caatttcctc gaatgccgga tgcgggccgc cgccgcgtac ggtcgacccc
      481 acgtacctgg aggcacaccc gtgacccacc acctgacact cccgggccgc gcgcggcgcg
      541 ccgctgtttc gatcctggcg atcctcgtcc ttcccgtgat cgcattcgcc cgtcccgtcc
      601 agtcgcccgg cctgtcagcc ttcaacgccg cggagcccga tcgccccggg tttgtcgtga
      661 acaatcttcc cgggagcggg ctggtcatcg acctgatggt ggaggggccg gcaaccgaca
      721 gcgaactcga ggccctgggg gccatcatcg ggagccgtct cccgaatggc gtgcgcaccg
      781 tgcggatgcc ggtggcacgc ctcgacgacc tccgcgccct gccgagcctc acgcgcctcg
      841 tcgcgtcacg gccggtcagg atgctcaacg attcctcggt gccgctcacg ctcgccacgc
      901 cgaactactg gacatccgcc ccgccgaact tcaccggttc ggccggtacc aacgtcatcg
      961 tcggcatcgt cgactcgggg atcgactggt cgcacgatga cttcaagaac cccgacggta
     1021 ccacccgcat cctgaacatc tgggaccaga acatcgccgg cgtgaacccg gccggcttcg
     1081 catacggcaa cgagtgcacg caggcccaga tcaacgcggg gacgtgcagc gaaaccgacg
     1141 cggtcggtca cggcacgcac gtggcgggaa gcgccgcggg cgacggttcg gccaccggaa
     1201 acggccagcc ggccaatcgc ttcatcggca tggcgccgcg ggccaacatc atcgtcgtgg
     1261 ccacgaattt cagcggcaac ggggtggtgg acggcgtgaa ctacatcttc cagaaggccg
     1321 ccgcgctcgg gcgacccgcg gtggtcaacc tgtcgctcgg cagccagtat ggcgcccacg
     1381 acggcaccga ggggctcgac accgccttga gcgcgttgac cggccccggc aagatcatcg
     1441 tggcttcggc cggtaacgag gggggttcgt cacggcatgc cgagcagctg gtaccgccgg
     1501 ggccggccca gacgatcacg ttcaacgtgc cggcctatgc ggccgcggtc ggcgcgaaca
     1561 acgattacgt gatcctcgac tgctactaca atggttcggc caacatgtcg gtcaccgtca
     1621 cgtcgcccgg tccgacgccc gtcgtgctgg gcccgatcac caaggggacc aacggcggca
     1681 gcggcgcttc gctggccggc acgatctacg ccgagaacgg cttcaccccc tcggcgtccg
     1741 gcgccaccaa catcctcatc caggtcttcg attccaactc ggcccgcacg ccccgggtgg
     1801 gcgtgtggac ggtgacactc acgccggtag ccaccgtggc ctccaccgag tgcgacctct
     1861 ggctcgcgga cttccacctg ggcaccgcgg gcgtcgcggc caccttcacc agcgacgtcg
     1921 agaacgccga actggtcggc actccgggca ccggcaacag catcatctcg gtggccgcct
     1981 tcacctccaa acagttctgg ccgtcaatcg acaccaacag ctactcgttc gtcggctcca
     2041 cgccggccgg cccgatcgcc ggattctcga gccccggccc gcgtcgcgac ggcgcgcaga
     2101 agcccgacat cgccgcgccc ggcacggcca tcgtgtcgtc ccgctccacg gccacggcgg
     2161 ggatccccaa cgccctcatc aaccccgacg gcgtccactg gacacagcag gggacgagca
     2221 tgtcctcgcc gcacgtgacg ggcgccgtgg ccctctacct ggccgacagc ccgacaatga
     2281 cgccggccca ggccaggacc aggctggccg ccgacgccct cgtggacggg aacaccggcg
     2341 ccgtgcccaa cgccacctgg ggcgccggca agctccggat gatccgcgcc gacgcggtcc
     2401 ccccgatggt cacccttacg gcgcccaacg gcgggcaggt cttcatcgaa ggatcgatgc
     2461 agaacatcac ctggaccgcc acggacaacg tgggcgtcac ggccatcgac ctggcctact
     2521 ccaccaataa cggcggagcc tggacgacga tcgtgaacgg cgagccgaat gacggcacct
     2581 acagttggac cgtgcccgcc gtcacgacca tccaggcgct cgtccgcgtg acggcccacg
     2641 atgccctcaa cacggcatcg gacgccagca acgcggtgtt tgccatcacc tcgctctccg
     2701 cggcgcccga tcgctcggcg cctccggtcc ggcccatcgc gctggccaac gcgcccaacc
     2761 cgttcggtgc gagcacgatc atcacgttcg ggcttccggc caacgaaaac gtgaagctct
     2821 cgatcgtgtc acctgccggc cgtctggtcc gccggctcct ggacggttcc ttcccggccg
     2881 gctggcacga cacggcctgg aacgggacgg cagacgacgg gcggcaggtg gcgcagggaa
     2941 tctacttcta caagttcgag gccggggacg tggtgacgac gcagcggatg gtgctgacaa
     3001 gataggcgag cgtcacgatc tggcgggagg cacgccggca acggcccgac ccggcccttc
     3061 tccggccggc cgggccgtcg ttatttcctg gtccttgtac tgcaactggt acagcttgaa
     3121 gtagagcccg ccgtgcgcca gcaactcccg gtgcgagccc tgctcgcgaa gctgcccctt
     3181 gtggagcacc aggatccggt cggcgtcctg gatcgtcgag aggcggtggg cgatgacgat
     3241 cgaggtccga ccggagagga ggcggtgcag cgcttcctgg atgagctgct cggtttcggt
     3301 gtcgaccgac gaggtggcct cgtccagcac cagcacctcg gggcgatggg ccagcgcccg
     3361 cgcgaaggcc aggagctgct tctggccggt ggagagcgag gcgccccgct cgccgaggcg
     3421 ggcgccgaga ccgtccggca gcgtgtcgat gaagcggccg gcgttcacgt ggcggatggc
     3481 gtcggccacg cgtccgtcgt cgatgtcacg gtcaccgagg cggatgttgt cggcgatcgt
     3541 gcccctgaag aggaagacgt cctggagcac gaggccgatg gaacggcgga gcgccgacac
     3601 gtcgagcgtg cggatgtcgt ggccgtccac caggatcgat cccttctgga tgtcgtagaa
     3661 gcggcagaag agggagatga tggtggtctt ccccgagccg gtggcgccga ccagggccac
     3721 ggtttcaccg tgccggaccg tgaacgacac gtcgcgcagc acccactcct cgtcgttgta
     3781 ggcgaaccag acgttccgga attcgatgct cgaggaaaat cccgaccagg gtgccggagc
     3841 gtccggcacc gggatcacct ggggcgcatc gagcatccgg aagatccgct ccgccgaagc
     3901 catggccgcc tgcaggatgt tgtacttctc ggagagatcc atgatcggcc ggtagaagcg
     3961 gcgcacgtac tggatgaagg ccaccaggac accgagcgac aggagcgatg ctccaccggc
     4021 atcccccatc gtgcgcaggc ctccgtacca caggacgagc gccagggcca ccgacgagat
     4081 cacttcgatc agcgggtaga acaccgcgta atagaagatc gaacggatgt gcgcgtcgag
     4141 gtggtcggcg ttgagcctgt cgaaatgccg gttgcggtga gcctctcgtc cgaagatctg
     4201 caccacgctc atcccggtga tgttctcctg gaggaacgtg ttgatgcggg cgatgcgcgt
     4261 gcggaccatc cggtacgtct cgcgcacccc gcggcggaac cacgcggtca cgatcacgag
     4321 gagcggcatc acgatcagcg tggccaacgt cagccgccag tccagccaga aaaggatccc
     4381 caggatcccg aagagagtga cgaggtcgcc gaagagcgcc accacgcccg aggtgagcat
     4441 ctcgttcagc acgtccacgt cggtggtcag gcgcgtgagc atgcggccca ccgggttccg
     4501 gtcgaaccag gcgctcgact gcccgagctg gtggcggaag atctgcttcc gcatctcgta
     4561 catcacgagc tgccccaccc gctgcatcag ccaggtctgg aaccaggtga ggaggagcgc
     4621 cccggccagg gcgccgaaga aggccacggt gaggaccagg aggtgccggt tgtcatgggc
     4681 cgggatggcc acgtcgatgg cctgctgggt gagccacggg ccggccagtt ccagcgccga
     4741 ggtggccagc aacaggatgg tggccaggat gacggcgccc cgatgcggcc ccaggtagcc
     4801 gagcaggcgc cccatcaacc gccggtcgaa cgccttgccg gtgaattcct cctcgggatg
     4861 cgcctcgggg ttcatgcggt ggcctcgatt ttcacgccgt ggcctcgatt tcctcgcgca
     4921 ggcgctggcg gcgctcgagg tcggcgtagc ggccgccgag cgtcaggagc tcgtcgtgag
     4981 tgcccgactc cacgatgcgc cccgcttcga gcaccacgat acggtcggcg tgccgcacgg
     5041 tggaaacacg gtgcgacacg atcagcgtcg tgcgcgtggc catcggctcc ctgagccggg
     5101 tcaggatgtc ctcctcggtc ttcgtgtcca cgctggagag ggcgtcgtcg aggaccagga
     5161 tgcgcgggcg ccggaggatg gcccgggcaa tggccgtgcg ctgcttctg
//
LOCUS       DASVOD010000619         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_458268, whole genome shotgun sequence.
ACCESSION   DASVOD010000619 DASVOD010000000
VERSION     DASVOD010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458268"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VF720_13505"
     CDS             <1..334
                     /locus_tag="VF720_13505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880426.1"
                     /translation="GKSSGRGDMTLAFKSVTDAGGKSHAIAAETFYAQAESGTDADVA
                     RVAGGAAVGAIIGGIAGGKKGAGIGGLIGAAAGTGTVLATAGPQVKLASGQVFDIKLI
                     EPVDVTKS"
     gene            377..1285
                     /locus_tag="VF720_13510"
     CDS             377..1285
                     /locus_tag="VF720_13510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX7880427.1"
                     /translation="MTANASIRARGLGGRSWRHLGAAVWRGVNRHDLTGMAAAVAYHS
                     IFSFFAGFFFLACVATQFGRSQENLEWILALLRNVLPPPGIRLAEENLARFLRPVSKE
                     ALPLALILSLWTASNVVEVVMRGLNRIYSVTETRPWWQTRIGAIVLTAMASFFFIVAF
                     NLTIFERQTVAGLDRLLHYRTILPELVHALQWPLIWLGSVGAAAVIYFLAPNFSRTQR
                     RIALPGAVFFAVVWFILNQGFTGYLANFGDFDRIYGPLGTAMAVLLWVYLSSLILLIG
                     GEINAEVGRVLPRKSKKQVPSAAEAA"
     gene            1310..2008
                     /locus_tag="VF720_13515"
     CDS             1310..2008
                     /locus_tag="VF720_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019055311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7880428.1"
                     /translation="MNTNATILLVEDDPRIAGVTRKNLEAAGFRCRTAADGPSALREM
                     ESAPPDLVVLDVMLPGLDGVEVTRRIRKSSRVPILMLTARDTEADKVLGLEVGADDYL
                     TKPFSLRELIARVRALLRRSNPPTDSAILVRGTLSIDPGERVVTVGGQRLELTALEFD
                     LLEFMARQPRRVFTRDALMNQVWGDDRVVDARSIDSLISRLRKKLGAIPGGSNCVSTV
                     WGAGYRFVEPGAGT"
BASE COUNT          328 a          713 c          767 g          371 t
ORIGIN      
        1 cggcaagagc agcggtcgcg gcgacatgac gctggccttc aagtcggtga cggacgcggg
       61 gggcaagtcg cacgccatcg ctgcggagac gttctacgcc caggccgaga gcggcaccga
      121 cgccgacgtg gcccgggtgg cgggcggggc ggcggtcggc gccatcatcg gcggcatcgc
      181 gggcggcaag aagggggccg gaatcggcgg cctgatcggc gcggcggccg ggaccggcac
      241 cgtgctggcc accgccgggc cccaggtgaa gctggcttcc gggcaggtgt tcgacatcaa
      301 gctgatcgag ccggtcgacg tgacgaaaag ctgaacccac gacgctcccg ggcccgagag
      361 gctaatatcg ccggccatga cggcgaatgc atcgatccgg gcccggggcc tgggaggccg
      421 ctcctggcga cacctgggag cggccgtctg gcgcggggtg aatcgtcacg atctgacggg
      481 gatggcggcc gccgtggcct accactccat cttcagcttc tttgccggtt tctttttcct
      541 ggcctgcgtg gccacgcagt tcggccggag ccaggaaaat ctcgagtgga tcctggcgtt
      601 gctgcgcaac gtgctgccgc ctccgggtat ccgcctggcc gaggagaacc tggcccgttt
      661 cctccgcccg gtctcgaagg aggcgctgcc gctggcgctc atcctctccc tgtggacggc
      721 ctccaacgtg gtcgaagtcg tgatgcgcgg actgaatcgc atctactccg tgaccgagac
      781 gcgcccctgg tggcagaccc ggatcggcgc catcgtgctg acggcaatgg cctccttctt
      841 cttcatcgtg gccttcaacc tgacgatctt cgagcggcag acggtggccg ggctcgaccg
      901 gctgctccat taccgcacca ttcttcccga gctggttcac gcccttcagt ggccgctgat
      961 ctggctggga tcggtgggcg cggcagccgt catttatttc ctggccccga atttctcacg
     1021 gactcagcgg cggatcgcgc tccccggcgc ggtcttcttt gcggtggtct ggttcatcct
     1081 gaaccaggga ttcaccggct acctggcgaa cttcggcgat ttcgaccgca tctacggacc
     1141 cctcgggacg gccatggcgg tgctcctctg ggtgtacctc tcgagcctca ttctcctgat
     1201 cggcggggag ataaacgccg aggtcggacg cgtgctgccg cgcaagtcga agaaacaggt
     1261 gccgtccgcc gccgaggcgg cctgagatga caagggtcgg atccgaccga tgaacaccaa
     1321 cgccacgatc ctcctggtgg aggacgaccc gcgcatcgcg ggggtgacgc ggaagaacct
     1381 ggaggcagcc ggcttccgtt gccggacggc cgccgacggg ccatcggcgc tgcgggagat
     1441 ggaaagcgcg cctcccgacc tcgtggtact ggacgtgatg ctcccggggc tggatggcgt
     1501 ggaagtgacg cggcgcattc gcaagagcag ccgggttccg atcctgatgc tgaccgcccg
     1561 cgatacggag gccgacaagg tgctgggcct cgaggtcggc gccgacgatt acctgaccaa
     1621 gccgttcagc ctgcgggagt tgatcgcgcg cgttcgcgcg ctcctgcgcc ggtcgaaccc
     1681 gccgaccgac agcgccatcc tggtgcgcgg aaccctctcc atcgatccgg gcgagcgggt
     1741 agtgacggtg ggtggccaac ggctggagct taccgcgctc gagttcgacc tcctggagtt
     1801 catggcgcgc cagccccggc gcgtcttcac gcgtgacgcg ctcatgaacc aggtgtgggg
     1861 ggatgatcgc gtggtggacg cccgctcgat cgacagcctg atcagccggc ttcgcaagaa
     1921 actgggcgcc atcccgggtg gatcgaactg cgtgagcacc gtctggggcg ccggctaccg
     1981 gttcgtcgag ccgggggccg ggacgtgagg gcgaagagcc tcttctggaa gctggccggc
     2041 acgctcctcc tcgtgctggt ggcggcgttc ctgttgcaca cggtgctgat cgtcctcttc
     2101 ttcgatcccc tggtcgagaa gtggaacgaa cagcgcgcgc ggaacatggc cggcacgctg
     2161 gcgcgcgagc tggagcggc
//
LOCUS       DASVOD010000620         8039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_352522, whole genome shotgun sequence.
ACCESSION   DASVOD010000620 DASVOD010000000
VERSION     DASVOD010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352522"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..984)
                     /locus_tag="VF720_13520"
     CDS             complement(40..984)
                     /locus_tag="VF720_13520"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX7880429.1"
                     /translation="MLARFIGFVASPRPDRLHEKPTPFLGGLALVLALAGGLVAAGRL
                     TGPTDGAPLGLANAPTVTLLFVLATFAFLLGLVDDWRTLSPPAKLSGQVALAVAFLSV
                     AGTGPFDSPVLDGVLGIIWIAGLMNACNFLDNMDGALGGAALPAAVGLGLLSVRPDTA
                     DLGMILGAAIAGFLAWNRPPARIFLGDSGSLLIGGALALTGWTIASESDALAGWLALP
                     VIVAWPIFDMTFVTVTRLRRGQSPWVGGRDHTTHRLATRLGSAIRAFFVVLLVSALAA
                     TLGVMSGRAGTTAAAFLLAGAIVAFGAAGVALSRVGVR"
     gene            complement(1056..2135)
                     /locus_tag="VF720_13525"
     CDS             complement(1056..2135)
                     /locus_tag="VF720_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7880430.1"
                     /translation="MKRILVTGGAGFIGSHLVESLLERGHEVRAVDNLSTGRLSNIRH
                     LTDHPRFHFTEGTVLDPELVERLVVDVDQIFHLAASVGVRYVVDNPLLSLKNNIHGAE
                     VILDAAVPRKVPVVLFSSSEVYGKGHGGPLHEDDDRILGATRYSRWGYAASKSVDEFL
                     AFAYHREMGLPIRLVRCFNTCGPRQVGSYGMVIPRFVEQALDGRDITIYGNGEQSRCF
                     SFVGDVVRGVLMLADHPEANGQVFNIGTDEEVTVRELAGRIVEKIGSDSRLEFIDYKD
                     AFGPGFEDMPRRVPDLSRINGLVGYRPEVSLDRLLDLTIENIRAERGMESPARNAHHV
                     TLPAAAGVAVAMSGAAGGVPVGDGD"
     gene            2500..3306
                     /locus_tag="VF720_13530"
     CDS             2500..3306
                     /locus_tag="VF720_13530"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7880431.1"
                     /translation="MAEVYVPILSYHRVVDGVDSPLAVTPAEFARQVAWLTDQGYTGV
                     PLADAVAARAGRLSGQAGGRGGAGKNFKAFTLTFDHGYADNFRNVFPILKERRIPAHF
                     FLVTEPIDSGSLLALPGNPNTDPKRDRLMNWDEAMELKEAGMGIGAMSARHVDLTALD
                     PAAARDEAEQSRDAIRGFLGDDPDYFSWPFGKFTPALKEMVKDVGFRGAVHTPTGKTG
                     GMDRFSLRRIGIRPGFSDKVFAYKVSEQADLLRENPAKFAMMKALGKAFD"
     gene            3389..3961
                     /locus_tag="VF720_13535"
     CDS             3389..3961
                     /locus_tag="VF720_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880432.1"
                     /translation="MILTTLLSLALIGCDEDESDDAPPLDVRGVYEVSWNGSLRDPET
                     SVYESDPSAVNGGGSGFVTVLSTDENILHGTFTILDGASPPFASVELPFTARMKRVYF
                     AIEDPVDYGWDLTVTSPVDDLVGCTYVGPVEVNSPGIIGVGRIDPELVINLAFGGIYD
                     CGPTRRHVIVGALGHRLGRAASRARTKVAG"
     gene            complement(3967..6549)
                     /locus_tag="VF720_13540"
     CDS             complement(3967..6549)
                     /locus_tag="VF720_13540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX7880433.1"
                     /translation="MFLIQVVEYLLIAGAAAWYWQKRADHGRDIAAHFTQLLLGLTAF
                     LAITAAWVVAASNLGPSRGAPPGVATLVQLLRVAAIGHAFLAAVMLAWPHLAAKKVRW
                     IGAILALWLAGARGGSIGVPAFFWLLIILRKSTWPQEIHGWRRLLGLILVPLAVAALF
                     MIPLTTFDKGVASVNFTPAPDPWPAPLMTGDVPQGVETELALIRPLDTVLSLFRSGLL
                     AQLLLVSIQFLTLPVRLRGVGIKRRLAINYFFIRVIPATLGVLSYVLLMWGAFGLHKA
                     AMVRVALEETIERCGEALDRLAAESFTTAGAPGDNDASARLASPRLSLGDDGPRARFL
                     RHDPEGVTTLGGPGDSLVVFSAADTVQRRRDGGLIAVGGGLWLYARRPIDGDSLRRFE
                     VYVPLDSVYLARVAARRHIDIRLSADPDLFIGPTSVTSGSDTTWTDEPVHVTGLYHEP
                     PATRQLLDRRFVLGQSFLPIGNWLSERQTGMSGAVTLKLNMAPWYVIPNRRDLVILVA
                     GNAWTIGLLLLVIVVVSVTEGMAVRTGRGILNALFDDVSALQVAAQRFGKGDLDYRLP
                     VHGKDEIATVAGAFNEMAASLKAQQAELVEKERLELDLKLARDIQRRLLPQAPPVVSG
                     VDLAGVSIPSLEVGGDLFAFFNEGPTRLGLALGDVSGKSVPAAILMSNTLAALRAEAE
                     LAGAVGGSLARLNRTMVEHVETGRFVTLVYALLDPTTGALEFASAGHNPALLARASGE
                     VEWLEASGPPLGVLPGAEYEPTSRKLAPGDVLVLYSDGITEAARLPDGGTVSAALSPE
                     EMEFFDESRLAEVVTRSRHLPAAGIMEAVLAAVHTFSGNQPQADDVTLLVVRYTGTTA
                     SDRG"
     gene            6708..7817
                     /locus_tag="VF720_13545"
     CDS             6708..7817
                     /locus_tag="VF720_13545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880434.1"
                     /translation="MKIALIINSAPPFPVSGSEQQGMTMAACLAARGHRVTLFARRYG
                     GAPVVDPMDGYTLIRVPFLDMGPLRFPSHCWNFDRLFARHGADADVILAYQTFAPGYL
                     GARAARRHRIPCVLWIRSTMGFMLSHSPRLTWAMRQSLPHIDHLLFQSERLMAPFLTE
                     VRHSLGDRLADRAAARMSVGRNAVAMRATEPSPGNELLVVGRLIELKDLPTLFRALRR
                     MKTPPPTRLVGDGPILAALQSEAAGLPVTFAGRMAPDQLPGEYRRARVLAMTSTTEGM
                     PNVILEAMASGVPVVSTPVGSIPDVVKPGETGYLFPVADDEALAGHLTRLFSDDPLRD
                     RLARGALNQAGQFSWERVTDGVEEVLGRVGMAPVG"
     gene            7855..>8039
                     /locus_tag="VF720_13550"
     CDS             7855..>8039
                     /locus_tag="VF720_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880435.1"
                     /translation="MTNAGRLSGGSSAGVRVGWYGLAQVANAGLAMLLLGILARSLGR
                     AGFGEFSFAFVVASLGA"
BASE COUNT         1388 a         2665 c         2772 g         1214 t
ORIGIN      
        1 gggtgctcgc gcgcgagccg agccggagcc gcgtgggctt catcgcaccc ccacacgcga
       61 gagcgccacg cctgccgcgc cgaacgccac gatcgccccc gcgaggagga aagcggccgc
      121 cgtggtaccc gcccgaccgg acatcacgcc gagcgtggcc gccagggccg agacgagcag
      181 cacgacgaaa aaggcgcgta tcgccgagcc gagccgcgtg gccaggcggt gcgtggtgtg
      241 gtcgcgcccc ccgacccagg gcgattgtcc gcgccggagc ctcgtcaccg tgacgaaggt
      301 catgtcgaag atcggccagg ccacgatcac cggcagggcg agccagccgg ccagggcgtc
      361 ggactccgag gcgatggtcc agccggtcag cgcgagcgcg ccgccgatga ggaggctccc
      421 ggagtcaccg aggaaaatgc gcgcgggggg tcgattccag gcgaggaagc cggcgatcgc
      481 ggcgccgagg atcatcccca ggtcggccgt gtcgggacgg accgagagaa gcccgaggcc
      541 cactgccgcc gggagtgcgg ccccgccaag cgccccgtcc atgttgtcga gaaaattgca
      601 cgcgttcatc agtcccgcga tccagatgat gccgaggacg ccgtcaagga cgggcgagtc
      661 gaacggcccg gtgccggcaa cggaaaggaa cgccaccgca agggccacct gccccgacag
      721 cttggccggc ggggagagag tacgccagtc gtcgaccagg ccgagcagga aggcaaaggt
      781 ggccaggacg aagaggagcg tgacggtcgg cgcgttggca aggccgaggg gcgcgccatc
      841 ggtcggaccg gtcaggcggc cggcagccac gagacccccc gcgagcgcca gaacgagtgc
      901 aaggcccccc aggaacgggg tcggtttctc gtggagacgg tccgggcgcg gcgatgccac
      961 gaagccgatg aaccgggcca gcatggcggc gagcggtgtg aggaggatcg cggcgaccag
     1021 ggcaacgaag gggccccggt ggatcagcga ctcgatcagt cgccgtcgcc gaccggaacg
     1081 ccgccggcgg cgccggacat cgccaccgcc acgccggctg ctgcgggaag cgtgacgtga
     1141 tgagcgttgc gcgcgggcga ttccatcccc cgctcggccc ggatgttctc gatcgtgaga
     1201 tcgaggagcc ggtcgagcga aacctcgggg cgatagccga cgaggccgtt gatgcgggag
     1261 agatccggca cgcggcgcgg catgtcctcg aacccggggc cgaaggcatc cttgtagtcg
     1321 atgaactcga ggcgcgagtc ggagccgatc ttctcgacga tgcgtccggc cagttcgcgc
     1381 accgtgactt cctcgtcggt gccgatgttg aagacctggc cgttggcctc gggatgatcg
     1441 gcgagcatca gcacgccgcg aaccacgtcc cccacgaagg agaagcagcg cgactgctcg
     1501 ccgtttccgt aaatggtgat gtcgcgacca tcgagcgcct gctcgacgaa gcgcgggatg
     1561 accatgccgt aggaaccgac ctgccggggg ccgcaggtgt tgaagcagcg caccagccgg
     1621 atcggcagtc ccatctcgcg gtggtaggcg aatgccagga attcgtcgac cgacttggag
     1681 gcggcgtagc cccagcgcga gtagcgggtg gcgccgagga tacggtcgtc gtcttcgtgg
     1741 agcggaccgc catgaccctt gccgtacacc tcggacgagg agaagaggac gaccggcacc
     1801 tttcgcggga cggcggcatc gaggatgacc tcggctccgt ggatgttgtt tttcagcgaa
     1861 agtagcggat tgtcaacgac atagcgcaca ccgacggatg ccgccaggtg gaaaatctgg
     1921 tcgacgtcga ccacgagccg ctcgaccagt tcgggatcga ggaccgtgcc ttcggtgaaa
     1981 tggaaacggg ggtgatcggt caggtggcgg atattggaca ggcgtcccgt ggagagattg
     2041 tcgacggccc ggacttcgtg ccccctctcg aggagggatt ccacgaggtg cgagccgatg
     2101 aaaccggcac caccggtgac gaggattcgt ttcaacctgg atccccctga tcgaagcatg
     2161 accgtggctt tctgccacgg atttcatgcg gggcctatga cgacgtgacg aacagattca
     2221 tcggctttgt ggccggatcg tttatcaaat tccgtcgtcc agggccctcc gcgaatcccg
     2281 taagtcattc aaatatcgtg gtgaacctac gggaccatca aaaaggatgt caaggggcta
     2341 tctcggctgc accacaggga tcggcttgct ccgaccccga ctcgagtata taaggtggcc
     2401 actccgttga catccggaca tccggcgtgg ccccgccctg tacgcgcagg accgctccgt
     2461 ttcagcacat tttccaaatc cggccgaaag gaatcgcccg tggcggaagt ctatgtcccc
     2521 atcctcagct atcaccgcgt cgtcgacggc gtggattctc cgctggccgt caccccggcc
     2581 gagttcgccc ggcaggtggc ctggctcacc gaccagggat atacgggggt tcccctggcc
     2641 gacgccgtgg cggcacgggc tggtcgtctc tccggccagg ccggcggacg cggtggcgcg
     2701 gggaagaact tcaaggcttt cacactgacg ttcgatcacg gctacgccga caactttcga
     2761 aacgtctttc ccatcctcaa ggaacgacga atcccggcgc acttcttcct ggtgaccgag
     2821 ccgatcgact cgggaagcct gctggccctc cccggcaacc cgaacaccga tccgaaacgg
     2881 gatcgcctga tgaactggga cgaggcgatg gagttgaagg aggccggcat gggaattggc
     2941 gcgatgtcgg cccgccacgt cgacctgacc gccctggatc ccgcggccgc ccgcgacgaa
     3001 gcggagcagt cgcgcgatgc catccgtggt ttcctcggcg acgaccccga ctatttcagc
     3061 tggcccttcg gaaagttcac gccggcactg aaggagatgg tgaaggatgt cggcttccgc
     3121 ggggcggtgc atacgcccac gggcaagacg gggggcatgg atcgcttctc gctccgtcgc
     3181 atcggcatcc gtcccggctt cagcgacaag gtgtttgcct acaaggtcag cgagcaggcc
     3241 gacctgctgc gggagaatcc ggccaagttc gccatgatga aagcgctggg gaaggcgttt
     3301 gattgaaagc ggcgcggcgg catgcggctc gccgcgcaca ttcgaaaggg ggccagtcat
     3361 gtcggttgtg ccactcgctc gacgagccgt gatcctcacg accttgctct ccctcgcact
     3421 cattggatgc gacgaggacg agtccgatga cgccccgccc ctcgacgtgc ggggcgtgta
     3481 cgaggtgagc tggaacggct ccctccgcga tccggaaact tcggtctacg aatcggatcc
     3541 gtcggcggtg aatggcggcg gttcggggtt cgtcaccgtc ctcagcaccg acgaaaacat
     3601 ccttcacggc accttcacga tattggacgg ggcttcccca ccgttcgctt cggtggagct
     3661 tcccttcacc gctcggatga agcgcgtgta cttcgcgatc gaggaccccg tcgactacgg
     3721 ctgggacctg accgtcacga gcccggtgga cgacctggtg gggtgcacgt acgttgggcc
     3781 cgtcgaagta aactcgcctg gcatcatcgg cgtgggacgg atcgatccgg agttggtgat
     3841 caatctggcc ttcggcggaa tctatgattg cgggcccacg cgacggcacg tgattgtcgg
     3901 ggccctgggg caccgcctcg gacgcgcagc ctctcgggcg agaacgaaag tagccggctg
     3961 acgacgtcag ccacggtctg atgcggtcgt ccccgtgtaa cggacgacca gcagcgtcac
     4021 gtcgtccgcc tggggctggt tgccgctgaa ggtgtgcacg gcggccagca cggcctccat
     4081 gattcccgcg gcggggagat ggcggcttcg ggtcacgacc tcggcgagcc gcgactcgtc
     4141 gaagaactcc atctcttccg gggacaaggc cgccgagacg gtgccaccat ccggcaagcg
     4201 ggctgcctcg gtgatgccgt ccgaatagag aaccaggaca tcacccgggg cgagcttccg
     4261 gctcgtcggt tcatattcgg cgcccggcag gacaccgagg ggcgggcccg acgcctcgag
     4321 ccactccacc tccccggagg cgcgcgccag gagcgccgga ttgtggccgg ccgaggcgaa
     4381 ttcgagggca ccggtcgtcg gatcgagcag ggcgtagacg agcgtcacga agcgcccggt
     4441 ctcgacgtgt tccaccatgg tgcggttgag gcgcgccagg ctccccccca ccgcgccggc
     4501 gagctccgct tcggcacgaa gtgcggccag cgtgtttgac attaggatgg cggccggcac
     4561 gctcttgccc gacacgtcgc cgagcgcgag gccaagccgc gtgggtcctt cgttgaagaa
     4621 ggcgaagaga tcgcccccca cctcgagcga cgggatcgag acgccggcca gatcgacacc
     4681 cgacacgacc ggcggtgcct gcggcaacag ccggcgctgg atgtcgcgcg ccagcttcag
     4741 gtcgagctcg agccgctcct tctccaccag ctccgcctgc tgcgccttga ggcttgccgc
     4801 catctcgttg aaagcgccgg ccacggtggc gatctcgtcc ttgccgtgca ccggcaggcg
     4861 gtagtcaaga tctcccttgc cgaatcgctg ggcggcaacc tgcagtgccg acacgtcgtc
     4921 gaagagcgcg ttcaggatgc cgcgcccggt gcggaccgcc atcccctcgg tcactgacac
     4981 gaccacgatg acgagcaata ggaggccgat cgtccacgca ttgccggcca ccaggatcac
     5041 gagatcccgt cggttcggga tgacatacca cggcgccatg ttcagcttga gcgtcacggc
     5101 gcccgacatg ccggtctgcc gctccgaaag ccagttgccg atcggaagaa aagactggcc
     5161 gaggacgaac cgccggtcca gcagctgtcg cgtggccggt ggttcgtgat agagcccggt
     5221 gacgtgcaca ggctcgtccg tccaggtcgt gtcgctgccc gacgtgacgg acgtggggcc
     5281 gatgaagaga tcggggtcgg cgctcaggcg gatgtcgatg tggcgccggg cggccacgcg
     5341 ggccaggtag acggagtcga gcggcacgta cacctcgaac cgccggagag agtcaccgtc
     5401 gatcggccgc cgggcgtaga gccacaggcc gccgcccacc gcaatgaggc ccccgtcccg
     5461 tcgacgctgg accgtgtcgg ccgcggaaaa gacgacaagc gaatcgccgg ggccgccgag
     5521 cgtggtcacc ccttccggat cgtgacggag aaagcgggcc cgcgggccgt cgtcaccgag
     5581 cgacaggcgt gggctggcca gccgggccga cgcatcgttg tcgccgggcg cacctgccgt
     5641 tgtgaaggac tccgccgcca gccggtcgag cgcctcgccg catctttcga tcgtttcctc
     5701 gagcgccacc cggaccatgg ccgccttgtg caggccgaat gcgccccaca tgaggaggac
     5761 gtacgagagg acgccgaggg tggcggggat cacgcggatg aagaagtagt tgatcgccag
     5821 ccggcgcttg attcccacgc cgcgcaggcg gacggggagc gtgaggaact ggatggacac
     5881 caggaggagc tgggccagca gaccgctccg gaagagcgaa aggacggtgt cgagagggcg
     5941 gatcagcgcc aactccgtct cgacgccttg cggcacgtca ccggtcatga gaggcgcggg
     6001 ccaggggtcg ggcgccggcg tgaaattgac cgaggccacg cccttgtcga acgtcgtgag
     6061 cgggatcatg aagagggccg ccaccgccaa cggcacgagg atcaagccga gcagccgtcg
     6121 ccagccgtgt atttcctgcg gccaggtcga cttgcggagg atgatgagga gccagaagaa
     6181 ggcgggcacc ccgatgctgc cgccccgcgc accggccagc cagagggcca ggatcgcccc
     6241 gatccagcgg accttctttg ccgcgaggtg cggccaggcc agcatgacgg cagccaggaa
     6301 ggcgtgtccg atggcggcga cccgcaggag ctgcacgagg gtggcaacgc ctggcggcgc
     6361 gccacgcgac ggccccaggt tcgacgcggc cacgacccag gcggcagtga tcgcgaggaa
     6421 ggccgtgagg ccgagaagca attgcgtgaa atgcgcggcg atgtcgcggc cgtgatcggc
     6481 acgcttctgc cagtaccagg cggccgcgcc cgcgatcagc aagtattcga cgacctggat
     6541 caggaacatg acggcaccgg acggcgcatc atcaccaggc ggcgcatcat catcgggcgg
     6601 cgcatcatcg cgcggcagta tagaccgggc tcgccgggcc tgatacggtc cgcccgggat
     6661 gacctgcatt gtccccatgc cccctgccca ggaaacgcgc acggcccgtg aaaatcgccc
     6721 tgatcatcaa ctccgcgccg ccatttcccg tctcgggttc cgagcagcag ggaatgacga
     6781 tggccgcctg cctcgcggcg cggggccacc gagtgacctt gttcgcccgc cggtatggcg
     6841 gcgctcccgt cgtcgatccc atggacggct acacgctgat acgcgtcccc ttccttgaca
     6901 tggggccgct ccgtttcccc agccactgct ggaacttcga ccgcctcttc gcaaggcacg
     6961 gcgccgacgc ggacgtgatc ctggcctacc agacctttgc gcccggctac ctcggcgcgc
     7021 gcgccgcccg gcgccacagg atcccgtgcg tgttgtggat ccgttccacg atgggcttca
     7081 tgctgagtca cagcccgcga ctcacctggg ccatgagaca gtcgctgcct cacatcgacc
     7141 acctcctctt ccagagcgag cggctgatgg ctcccttcct gaccgaggtg cgtcactcgc
     7201 tgggggatcg cctggcggat cgtgccgccg cccggatgtc ggtgggacgg aatgccgtag
     7261 ccatgcgcgc caccgaacca tctccgggga acgaactgct cgtggtcggt agactcatcg
     7321 aactcaagga ccttcccacg ctcttccggg ccctgcgacg gatgaagaca ccaccgccta
     7381 cgcgactcgt cggcgacggg cccatcctcg ccgccctgca gtccgaggcc gccggcctcc
     7441 ccgtgacctt cgccgggcgc atggccccgg accagctccc cggcgagtac cgccgcgccc
     7501 gcgtcctggc catgacctcg accacggaag ggatgcccaa cgtgatcctc gaggcgatgg
     7561 cctcgggcgt gccggttgtc tccacgccgg tcggatcgat ccccgacgtg gtgaagcccg
     7621 gcgagacggg ctacctcttc cccgtggccg atgacgaggc actggccggc cacctcacgc
     7681 gcctcttttc cgatgacccc ctgcgagacc gcctggcccg cggcgcgctg aaccaggcgg
     7741 gacagttctc gtgggagcgg gtgacggacg gggtggagga ggtgctgggg cgggtgggga
     7801 tggctcccgt ggggtaagcg ctcatgccgg cggccgctgt ggcatgatcc cgggatgacc
     7861 aacgcaggac ggttgtcggg cggatcgtcc gcgggcgtgc gggtcggctg gtatggactt
     7921 gcccaggtgg ccaacgccgg gctggcgatg ctcctcctcg gcatcctggc ccgttcgctt
     7981 ggcagggcgg gcttcggcga attctccttc gcgttcgtag tggcgtcact cggtgcgtt
//
LOCUS       DASVOD010000621         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_43610, whole genome shotgun sequence.
ACCESSION   DASVOD010000621 DASVOD010000000
VERSION     DASVOD010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43610"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..115)
                     /locus_tag="VF720_13555"
     tRNA            complement(40..115)
                     /locus_tag="VF720_13555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(80..82),aa:Val,seq:cac)
     gene            complement(134..209)
                     /locus_tag="VF720_13560"
     tRNA            complement(134..209)
                     /locus_tag="VF720_13560"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(174..176),aa:Ile,seq:cat)
     gene            397..1455
                     /locus_tag="VF720_13565"
     CDS             397..1455
                     /locus_tag="VF720_13565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7880436.1"
                     /translation="MRRLILVGTTITLCAATNLPSPQAIRASRAGCPEDPRLTLGAFG
                     RLSALDTDLPVNTGIGGGWRIGLRLFNDWYGEGDLSINKDFEDGPELRFTPIHLRLVR
                     QWHDGETTRYRLGLGYLHTSYEGGAGDDLREDDPLHFDGTDNGVTGSVGFERDIGQLA
                     ALRVDAVLDANLDGTLAADGNPDWHLGLEVGFNFGFGAGRRVDGDEDRDLILDSKDLC
                     PGTPLGTTVDGDGCPILIPEGSVTVVLDGVNFEVDTEHLLPESRAVLDRVAASLVAHP
                     ATAVEIRGFTDNTGDPVRNRELATARANTVRAYLIAKGANPDRLTAVGIGPDNPIDTN
                     ETEAGRARNRRVELKRTG"
     gene            complement(1601..>2253)
                     /locus_tag="VF720_13570"
     CDS             complement(1601..>2253)
                     /locus_tag="VF720_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880437.1"
                     /translation="LRATTTGEIYVMKRITTMSCLAAAVLATALAGTPVGATPPNPSP
                     GTAVVDGNPGEWNLATDFFTNMYRAGNPSKPIESKAYLRYDCSTHTMYVLVLGVPGVP
                     VYIDSNAVTAWVAIDNQSNKVVNEASGNDGIPPDFEWIGRNFDSNNQHALGYEASFPL
                     AEGSCNIIVHCDVDNGGAQTSASPGFPGTGPPLVVACDPVPTVTSTWSGVKKLLLP"
BASE COUNT          385 a          758 c          738 g          372 t
ORIGIN      
        1 cttccgtgga ggaagtgatg cggatgtgca accgggtagt ggtgggcgat acaggacttg
       61 aacctgtgac ccctcgcatg tgaagcgagt gctctaccag ctgagctaat cgcccggatt
      121 ccgtcggacg cggtggtggg ccctccagga tttgaacctg ggaccaaccg gttatgagcc
      181 ggccgctcta accgctgagc taagggcccg cgaaggccgc ttggcggcgg cggaccgccg
      241 tacaaggcgt ccgaatggaa agcgcggaac ggtatcatgg ggttccggga gcgtcaagcg
      301 agcgcgccgc gcgtggcggt gcactccctc catcctgatt tccacatagg gcccttcccc
      361 atttcccgcc cccatccccc tcgtcatgat cattccatgc gccgcctgat actcgtcggc
      421 accaccatca ccctgtgcgc ggcgacgaac cttccgtcgc cccaggccat tcgggcgtcg
      481 cgcgccggct gtcccgaaga cccgcgcttg accctcggtg ccttcggccg gttgagcgcc
      541 ctcgacaccg acctcccggt caacaccggc atcggcggtg gctggaggat cggcctccgc
      601 ctgttcaacg actggtatgg ggagggagat ctttcgatca acaaggattt cgaagacggg
      661 cccgagctcc ggttcacgcc gatccacctg cgactcgtgc gccagtggca tgacggcgag
      721 accacccgct accggttggg cctcggctac cttcacacga gttacgaagg aggcgcgggg
      781 gacgatctcc gcgaggacga cccgctccat ttcgacggaa cggacaacgg cgtcaccggc
      841 tcggtcggct tcgagcgaga tatcggtcag ctggccgcgc tgcgtgtgga tgccgtgctc
      901 gacgccaacc tcgacggcac gctggccgcg gatggcaacc cggactggca tctcggcctc
      961 gaggtggggt tcaacttcgg attcggcgcc ggacgccgcg tggacggcga cgaggatcgt
     1021 gacctgattc tcgactcgaa ggacctctgc cccggcacgc cgctcggcac caccgtcgac
     1081 ggcgacggct gcccgatcct catccccgag ggatcggtca ccgtggtgct cgacggcgtc
     1141 aacttcgagg tcgacaccga gcacctcctc cccgaatcgc gcgccgtcct cgaccgcgtg
     1201 gccgcgagcc tcgtggccca cccggccacg gccgtcgaga tccgcgggtt caccgacaac
     1261 accggcgacc ccgtccgcaa ccgagagctg gcaaccgcac gcgccaacac cgtccgtgcc
     1321 tatctcatcg cgaagggcgc caaccccgat cgactcaccg ccgtcggcat cgggccggac
     1381 aatccgatcg acacgaacga gacagaggct ggtcgggcgc gcaatcggcg agtggaattg
     1441 aagaggacgg ggtagcgcgg ccgcaccttc cagccatcga gttcggggtc cagcacgctt
     1501 cgccggacgc ggcgagtcgc cggcgctgat ttgcacacaa gaaaacgggc gaccccagca
     1561 gtgggggccg cccgtgcgga gcttccgatc gaaacgggga ctacggcagc agcagcttct
     1621 tcacccccga ccaggtcgag gtcacggtcg gaaccggatc acacgccacg accagcggcg
     1681 gacccgttcc cgggaatccg gggctggccg acgtctgggc gcctccgtta tccacgtcgc
     1741 agtgcacgat gatgttgcag ctcccctcgg ccaacgggaa cgacgcttcg tagccgagcg
     1801 catgctggtt gttgctgtcg aagttgcggc cgatccactc gaagtccggc ggaatgccgt
     1861 cgtttccaga ggcttcgttc acgaccttgt tcgactggtt gtcgatggcc acccatgccg
     1921 tgacggcatt ggagtcgatg tagacgggga cgcccggcac gccgagcacc agcacgtaca
     1981 tggtgtgcgt gctgcagtcg taccggagat acgccttcga ctcgatcggc ttggaaggat
     2041 tgccggcgcg gtacatgttg gtgaagaaat cggtcgccag gttccactcg cccggattac
     2101 cgtcgacgac ggccgtgccg ggcgaggggt tcggcggggt ggcaccgacg ggcgtgccgg
     2161 cgagcgccgt ggcgaggacc gcggcggcaa ggcatgacat ggtcgtgatt cgtttcatga
     2221 cgtagatctc ccctgtagtt gtcgcacgca atg
//
LOCUS       DASVOD010000622         1542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_146890, whole genome shotgun sequence.
ACCESSION   DASVOD010000622 DASVOD010000000
VERSION     DASVOD010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146890"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VF720_13575"
     CDS             <1..1128
                     /locus_tag="VF720_13575"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880438.1"
                     /translation="EDQYLEDLGLATALGLGGANAEGTRYDRFDNQTPSSAQLSFGRP
                     LRSKYGCSQTQVFAYQAIAWHSGTLTSGVLTTQDAAILNPWLTLSIGAPRRFWGSGEG
                     IMQSMQNEGGLARSFLNNTLGVLQSCTGIRLAGCGGAAIDTTYCLPTSPVVGAHFASS
                     VLSRARGNGCPNLHAFDVLATNPAVTSARGQWNYFRSGGPVSYASVTNVSTGPSAYRS
                     VLDGISVGRLRTNPGYYFNGCDDVGASLLRTSDVLNWFGAATLCKASAALSEVPEVGQ
                     PPAAPPAVAVLGNARPNPMVRHTIISMTQGASATPVRLDIFDVTGRLVRCLVDGPLAA
                     GPHEFAWDGRSAEGHPVAGGLYFYRMSAGDRVESRKLLVVD"
BASE COUNT          254 a          517 c          519 g          252 t
ORIGIN      
        1 gaggaccagt acctcgaaga cctcggcctg gccaccgcgc tggggttggg tggcgccaat
       61 gccgagggta cccgctacga ccggttcgac aaccagacgc cgtcatccgc ccagctctcg
      121 ttcggccgtc ccctgagatc gaaatacggt tgcagccaga cccaggtgtt tgcctaccag
      181 gcaatcgcct ggcactcggg cacgctgacc agcggagtcc tcacgaccca ggatgccgcg
      241 atcctgaacc cgtggctgac actttcgatc ggcgcgcccc gccgtttctg gggttccggc
      301 gaagggatca tgcagtcgat gcagaacgag ggtggcctgg cgcgatcgtt cctgaacaac
      361 acgctcggtg tcctccagtc gtgcaccggc atccggttgg ccggttgcgg cggtgcggcc
      421 atcgacacga cctactgcct tcccacgtcg cccgtcgtgg gggcccactt cgcctcgagc
      481 gtcctgtcgc gggcccgggg gaacgggtgt cccaacctcc acgccttcga cgtcctggcc
      541 acgaatccgg ccgtgacgtc ggcgcggggg cagtggaact acttccgaag cggcgggccg
      601 gtcagctacg cgtccgtcac gaacgtgtcg acaggtccct cggcgtatcg cagcgtgctc
      661 gacgggattt ccgtcggccg gctgcggacg aatcccggct actacttcaa cgggtgcgac
      721 gacgtcggcg cgtcattgct ccggacaagc gacgtgctga actggttcgg cgcggcaacc
      781 ctttgcaagg catcagcagc gttgagcgag gtgcccgagg tgggtcaacc gccggcggct
      841 ccacccgccg tggcggtgct tggcaacgcc cggcccaatc ccatggtgcg ccacaccatc
      901 atctcgatga cgcagggggc ttcggccacg ccggttcgac tcgatatctt cgatgtcacc
      961 ggtcgcctcg tccgttgcct ggtcgatggg ccgctcgcgg ccggtccgca cgagttcgcg
     1021 tgggatgggc gctctgcgga ggggcacccg gtggccggcg ggctctactt ctaccggatg
     1081 tcggcggggg accgggtgga gtcgcgaaag ctgctggtag tggactgagg agccggaagc
     1141 tgcgccgggt gtcgtgaggt gttgccagca acagcggact gtatggcgta caggttcgag
     1201 aactcggttc aggcagagtt ccggcacgcc cgactcggtt caggcagagt tccggcacgc
     1261 ccaaccaggt tcaggcagag tccaacgtgc ctcgacaccg cccacgcgcc ccgacaccgc
     1321 ccacgcacca ctgtaccccc acctgctaca ctctcccgcg ccaagtcgaa cggacggtcg
     1381 cggcggactt cgggttcgtc gaggaaagtc cgaactccac agggccaggt gctgggtaac
     1441 ccccagcggg agtgatccca gggaaagtgc aacagaaagc agaccgcccg tcggggacgg
     1501 tccggatggc ggaacgggag gcatcgaaag gtgcgtccgg ac
//
LOCUS       DASVOD010000623         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_43706, whole genome shotgun sequence.
ACCESSION   DASVOD010000623 DASVOD010000000
VERSION     DASVOD010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43706"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VF720_13580"
     CDS             <1..544
                     /locus_tag="VF720_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX7880439.1"
                     /translation="GLLIHQFVVYSLAVRLLGGMSPAAFFRGVWEAMLTAFSTASSNA
                     TLPTSIRVAEENLKLPPAISRFVLTIGSTANQNGTALFEGVTVLFLAQFYGVELALGQ
                     QALVVGVCILGGIGTAGVPAGSLPVIALICGMVGVPVEGIGVILGVDRFLDMCRTTLN
                     VTGDLAAAVVVSRGEARTIA"
     gene            638..2044
                     /locus_tag="VF720_13585"
     CDS             638..2044
                     /locus_tag="VF720_13585"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880440.1"
                     /translation="MNRPIRARALAVVLSGSIALCGAPALAQAPDSPWSVTRLGLVYQ
                     TAGMRSVRVTEGIEYARTGGAPRTLDLYAPADGGEPRGAVIFVNVNADETNPPRRWAI
                     YRDWCRAVAAEGMIGIVYGSAPNQFDESLAALFEWIAANGNAHGIDPSRLGLYASSAN
                     VSLGLPFAMDRKPAGLRCAVYYYGGVDVDTLRDDLPVFYVKAQKDNPNLNRAIDGLWD
                     RARRDNLPWTMVVGRELPHAFDGVDASRPSVVIIRQTLDFWRAHLQELPPLLPLSTAR
                     EVNRFLYGQEHAEAARLLEQEAARDTSDVEVERLLLLSYRNLNDTAKGIPLAEKLIRR
                     APNEPNFRTSYGMLLATAGRQADALTQLETAARLGAQDFNTYFQLMLVAMGANKPDLA
                     VQYGTRAVAIFPTQGVAHYNLACAYVRSGNNEAAIASLEAAVRNGFRDRNTFDTDPDM
                     APLRQDPRYQALVASLGN"
     gene            2111..3865
                     /locus_tag="VF720_13590"
     CDS             2111..3865
                     /locus_tag="VF720_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880441.1"
                     /translation="MFHIHRNCIHLTSSILVLISALGVANIGMSAIVALAAPPAPGAP
                     KVTRHATVPETLPDGRHRALWVDFGVREPASTKAINYDGTAPSDVFAWTGGMVRLVRR
                     EGPATRPGAGDFYEGEDAKFRVAVNTEAPCRLLVTLGDATSARGPVAVGVDGGASTTV
                     RTAAGEFVDVAMDLLPEDGEVAVHLKALECASFAAVSVAVYVPPGARAVHALFHDPPP
                     HATADTRTLLRATTDFLLEHRPAEGCFSYDGSWYQSSYSIRTLFGAARVLNDPTLLSP
                     ATECLDRFVDEQGQDGAWAAGYFGKPKCALARRTASKHASANLADVGAAVLALEVAAG
                     QCTGPRRERFIGAARRFADKVALPNQLAGGAFPNLRFDGRVSKLPYSVATGLQAADLA
                     ALYQLTGRPQYLSAAEAAARHLVAGFLPDGRFRFEPHDTADVRVVSPCRFGDLYYVFE
                     GLIWVRHVTNDGKLATSISRVEDAALWGSAGIAAARTNTALWKPANFWEASKQGALLV
                     LLVDAREASIGDDAMQHRCQEWIDALVTWESDPARMRTLGALVHPASAEGRLALPATG
                     FAGIGLVAAAERNALFIR"
BASE COUNT          605 a         1455 c         1236 g          624 t
ORIGIN      
        1 cggcctcctc atccaccagt tcgtcgttta ttcgctggcg gtgcggctcc tcggcggcat
       61 gagtccggcg gccttcttcc gaggcgtgtg ggaagcgatg ctgacggcct tctcgaccgc
      121 ctcgagcaac gccaccctgc ccacctccat ccgcgtggcc gaggagaacc tgaaactgcc
      181 gccggccatc agccggttcg tcctgaccat cggctccacg gccaaccaga acggcacggc
      241 gttgttcgaa ggggtcaccg tcctctttct ggcgcagttc tacggcgtgg agctggcgct
      301 cggacaacag gcgctcgtcg tgggtgtctg catcctcgga ggcatcggca ccgccggcgt
      361 tccggcgggc tcgctgccgg tcatcgccct gatctgcggg atggtcggcg tccccgtcga
      421 ggggatcggc gtcatcctgg gcgtcgaccg cttcctcgac atgtgccgca ccacgctcaa
      481 cgtgacggga gacctggcgg cggccgtggt cgtctcgcgc ggcgaggccc gcaccatcgc
      541 ctgaatttcc cggccccggc tggttgaaca ctcccggtcg cgctaccgtc ttcctgcgtc
      601 cgatctccgg cccccgttcc cgtccgagga aatcgccatg aatcgtccga tccgcgcccg
      661 tgccctggcc gtcgtccttt ccggctcaat cgccctctgc ggcgccccgg ctctggccca
      721 ggctcccgat tccccctgga gcgtcacgcg gctcggactc gtttatcaga cggcgggcat
      781 gaggagcgtc cgcgtcaccg aagggatcga gtatgcgcgg accggcggcg cgccccgcac
      841 tctcgatctc tacgcgcccg cagacggtgg cgagccgcgc ggggcggtga tcttcgtcaa
      901 cgtgaacgcc gacgaaacga acccgccccg tcgatgggcc atctaccgcg actggtgccg
      961 cgctgtggcg gccgagggaa tgatcggcat cgtgtatgga tcggcgccca accagttcga
     1021 cgagagcctg gcggcactct tcgagtggat cgcggccaac ggcaatgccc acggcatcga
     1081 cccgtcgcgg ctcgggctct atgccagttc ggccaacgtg agcctgggac ttccgttcgc
     1141 gatggatcga aagcccgccg ggctccgctg cgccgtgtac tactacggcg gcgtggacgt
     1201 cgacacgctc cgcgacgacc tgccggtgtt ctacgtcaag gcgcagaagg acaatccgaa
     1261 tctcaaccgt gcgatcgacg ggctctggga ccgcgcccgc cgcgacaacc tcccctggac
     1321 gatggtggtg gggcgcgaac ttccccacgc cttcgacggc gtcgacgcct cgaggccgag
     1381 cgtggtcatc atccgccaga cgctcgactt ctggcgcgcg catctgcagg aactccctcc
     1441 gctgctcccc ctgagcaccg cccgcgaggt caaccgcttc ctgtacggcc aggagcacgc
     1501 cgaagccgcg cgcctcctcg agcaggaggc cgcccgcgac acgagcgacg tggaagtgga
     1561 gcgcctcctc cttttgtcgt accgcaacct caacgacacg gcaaagggaa tcccgctggc
     1621 cgagaagctg atccgccgcg cgccgaacga gcccaacttc cgcacgtcgt acggcatgct
     1681 gctggccacg gccggacggc aggccgacgc cctcacgcaa ctcgaaacgg cggccagact
     1741 cggcgcgcag gacttcaaca cctatttcca gctgatgctg gtagccatgg gcgccaacaa
     1801 gcccgacctg gcggtgcagt acgggacccg ggctgtggca attttcccca cccagggggt
     1861 ggcgcactac aacctggcct gcgcctacgt ccgcagcggc aacaacgaag ccgccatcgc
     1921 ctccctcgaa gcggccgtgc gcaacggctt ccgcgacagg aacaccttcg acaccgaccc
     1981 cgacatggcc ccgctccggc aggacccgcg ttaccaggca ttggtggcga gcctcgggaa
     2041 ctgagggaca agcgaaaaac atcgttcgta cgattgcccg accggcgatg aggcgggcat
     2101 actcgatcgc gtgttccaca tccaccgcaa ctgcatccac ctcacatcga gcatcctcgt
     2161 cctcatcagc gcattgggtg tggcgaacat cggcatgtcc gcgatcgttg ccctggccgc
     2221 tccgcccgca cccggcgcgc cgaaggtcac gcgccacgcc acggtccccg aaacgcttcc
     2281 cgacggccgc caccgggcgc tctgggtcga cttcggagtc cgcgagcctg cgtcgacgaa
     2341 ggccatcaac tacgacggca ccgcaccgag cgatgtcttc gcctggaccg gcggcatggt
     2401 gcgactggtc cggcgcgagg gtcccgccac ccggccgggc gccggcgatt tctatgaggg
     2461 agaggacgcc aaattccggg tggccgtgaa cacggaagcg ccgtgtcgcc tcctcgtgac
     2521 gttgggcgat gcgacctctg ccagaggccc ggtcgccgtc ggcgtggacg gcggcgcaag
     2581 cacgaccgtg cgcacggccg ccggcgaatt tgtcgacgtg gcgatggacc ttctccccga
     2641 ggatggcgag gtggccgtcc acctgaaggc actggagtgc gcatcattcg ccgccgtttc
     2701 ggtggcggtc tacgttccgc ccggcgcccg ggccgtccac gccctcttcc acgacccgcc
     2761 gccccatgca accgccgata cgcgcacgct gcttcgcgcc accaccgact tcctcctcga
     2821 acaccgcccg gccgaaggat gcttctccta cgatggcagc tggtaccagt cgtcgtattc
     2881 catccgcacc ctcttcggcg ccgcgcgcgt gctgaacgat cccacgctct tgtcgcccgc
     2941 caccgagtgc ctcgatcgat tcgtcgacga gcagggccag gacggcgcct gggccgccgg
     3001 ctatttcgga aagccgaagt gcgcgctggc ccggcgcacc gcctcaaagc acgccagcgc
     3061 caacctggcg gacgtcggcg ccgcggtgct ggcccttgag gtagccgccg gccaatgcac
     3121 cggcccgcgg cgcgagcgct tcatcggcgc ggcccggcgc ttcgccgaca aggtggccct
     3181 gccgaaccag ctggcaggcg gtgcttttcc gaacctccgc ttcgacggcc gcgtatcgaa
     3241 gctcccctac agcgtggcca ccggcctgca agccgccgac ctcgccgcgc tctaccagct
     3301 taccggccgg ccacaatatc tttccgcggc cgaagccgca gcccgccacc tggtggccgg
     3361 cttccttccc gatggtcgtt tccgcttcga accgcacgac accgccgacg tccgcgtcgt
     3421 cagcccctgc cgcttcggcg atctctacta tgtcttcgaa ggtttgatct gggtgcggca
     3481 cgtcaccaac gacggaaagc tggccacctc gatcagcagg gtcgaagacg ccgccctctg
     3541 gggctcggcc ggcatcgccg ccgcgcgtac gaacaccgcg ctctggaagc ccgccaactt
     3601 ctgggaagct tccaagcagg gagcgcttct cgtcctcctc gtcgacgcgc gcgaggcctc
     3661 gatcggcgac gacgccatgc agcatcgctg ccaggaatgg atcgacgcgc tggtgacgtg
     3721 ggaatccgat ccggcgcgta tgcgtacact cggggcactg gtccacccgg cttcggccga
     3781 gggacgcctg gcccttccgg ccacgggatt cgccgggatc gggctggtgg ccgccgcgga
     3841 acggaacgcc ctgttcatcc gctgacggaa cggaacgccc tcaggccagc agcgccgccg
     3901 gattgagcgg cttgagctcg
//
LOCUS       DASVOD010000624         2113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_146994, whole genome shotgun sequence.
ACCESSION   DASVOD010000624 DASVOD010000000
VERSION     DASVOD010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146994"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="VF720_13595"
     CDS             complement(<1..1349)
                     /locus_tag="VF720_13595"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX7880442.1"
                     /translation="MVIGKGCGIEVALFPLLVLLGCEGGCGRNDPGPGGVTVILATPR
                     DTTAALPASDPRLAASPGGSVDEPAAPNSVDVIFNQPMVAVGATTASVTRPPFTIRPR
                     VAGRYRWIGTRTASFVPDEPFAPATRYEGRVPGGLRSVNGRSVAADYTWSFETARPRV
                     TRALPDAEGAPVGPEVRLVLDFNQPVDPTLVRRASRLVALDGGTIGLDARRAGPEDEE
                     LLRYWDPDATGRRVVLVPRRRLKLGTEWRLEVSAGLKGTEGPLPMRDTWTRTFRTYGP
                     FTADSLLASRWQTDAVTLVLSNPVDTDTLLTHLTTEPKVTLRGAWQEWDTHRVTLSGD
                     FRAGRTYRLTIRPGLVDVYGQPLNAGGPISLGPVTLPDAAPGVAIRPNEAVLERTDRR
                     EIVVLLTNVGSARLEMTRVAAPRIGPVVRGRPKDEPSFRPGADGVVVRTLTKRTPWN"
     gene            1536..1979
                     /locus_tag="VF720_13600"
     CDS             1536..1979
                     /locus_tag="VF720_13600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7880443.1"
                     /translation="MIRAISAAETRPLRQAVLRPHQRVEELVYPGDDDPRTHHLGAFD
                     GGRLVGIASIYVEPMVAMPDAGPTDWRLRGMATAPEMRGTGLGGALLEACIEHSRNHG
                     GTRLWCNARVPAAGFYLRYGFVVHGEEFDLPGIGPHYLMSRRIGA"
BASE COUNT          325 a          742 c          736 g          310 t
ORIGIN      
        1 tcgttccatg gcgtccgctt cgtcagcgtg cggacgacga ctccgtcggc ccccgggcga
       61 aacgacggct cgtccttcgg gcggccgcgc accaccgggc cgatccgggg cgcggccacg
      121 cgcgtcatct cgaggcgcgc gctcccgaca ttcgtgagga gcaccacgat ctcgcgccgg
      181 tcggtcctct cgaggaccgc ttcgttgggc cggatggcca cgcccggggc ggcgtccggc
      241 aacgtgacgg ggccgagcga gatcggcccg ccggcgttga gcggctggcc gtacacatcc
      301 acgagccccg ggcggatcgt gagccgatag gtgcgaccgg ccctgaaatc acccgaaagc
      361 gtcacgcgat gcgtatccca ctcctgccag gcgccccgca gggtgacttt cggctcggtc
      421 gtgaggtggg tcagcagcgt atcggtatcg accgggttcg agaggacgag cgtgacggcg
      481 tcggtctgcc agcggctggc caggaggctg tcggccgtga aggggccata ggtgcggaag
      541 gtgcgggtcc aggtgtcgcg catcggcagc ggaccctctg ttcccttcag gcccgcgctc
      601 acctcgaggc gccactccgt cccgagcttg agacgccgcc gcggcaccag cacgacacgc
      661 cggccagtgg cgtccgggtc ccagtagcgg aggagctcct cgtcctccgg gccggcccgc
      721 cgcgcatcga gcccgatcgt gccgccgtcc agagccacga ggcggctcgc ccgccggaca
      781 agggtgggat cgaccggctg gttgaaatcg aggacgagtc gcacctcggg gcccacgggc
      841 gcgccttcgg catcgggaag tgccctcgtc acccgcgggc gggctgtctc gaaggaccag
      901 gtgtagtcgg cggccaccga tctgccgttg accgaacgga ggccccccgg tactcgaccc
      961 tcgtagcgcg tggccggcgc gaagggctcg tcgggaacga agctggccgt gcgcgtaccg
     1021 atccaccggt agcgccccgc cacgcgtggc cggatcgtga agggcggacg ggtgaccgac
     1081 gccgtcgtgg ctccgaccgc caccatcggc tgattgaaaa tgacatcgac cgagttgggc
     1141 gcggcgggct catcgacaga accgcctgga gacgcggcaa ggcgcgggtc gctggcggga
     1201 agcgcggccg tcgtgtcacg cggggtggcg aggatgaccg tgacgccgcc cggtcccgga
     1261 tcgttccgac cgcacccacc ctcgcagccg agcagaacga gcagtggaaa gagcgccacc
     1321 tcaattccgc aacccttgcc gatcaccacg acctccctcg ctccgggact ggaaactgta
     1381 tcgatactgg atgtgactca ggctgcggcg gccgacggca agtgtagtgt gacatcagta
     1441 ccccaacaat cggccgcgag gccggccgcc ggcgtgacgg ggcgttcccc atcatgcccg
     1501 agcgttcccc agtatggtat gaatcgccga tgcccatgat ccgagcgatc tccgccgccg
     1561 aaacccgtcc cctccgccag gccgtgctcc gaccccatca acgggtcgag gagttggtct
     1621 acccgggtga cgacgatccg cgcacgcatc acctgggcgc gttcgacggt gggcggctgg
     1681 tcggcatcgc gtcgatctac gtcgagccga tggtggcgat gcccgacgcg ggcccgaccg
     1741 actggcgtct ccgcggcatg gccacggcgc cggaaatgcg aggtacgggg ttgggcgggg
     1801 cgttgcttga ggcctgtatc gaacacagtc gcaaccacgg cggcacccgc ctctggtgca
     1861 acgcgcgcgt gccggcggcc ggtttctatt tgcgatacgg attcgttgtc cacggggagg
     1921 aattcgacct ccccggaatc ggaccccact acctgatgag ccggcggatc ggagcgtagg
     1981 ctccgcgcca ggcgtccggc tcgaagcgcg cgtctccgat gtcagtcgac ccggcgtttg
     2041 tcgacctcac gtcagtcgac ccggcgcccc tcgtccacga ccttccgccc gccgatccag
     2101 accgagcgga cat
//
LOCUS       DASVOD010000625         2453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_823441, whole genome shotgun sequence.
ACCESSION   DASVOD010000625 DASVOD010000000
VERSION     DASVOD010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823441"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /gene="deoC"
                     /locus_tag="VF720_13605"
     CDS             <1..422
                     /gene="deoC"
                     /locus_tag="VF720_13605"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016380614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX7880444.1"
                     /translation="IDMVINVGLLKAGRDAEVEADIRALADACHDSGARLKVIIEAAL
                     LNDEEKVRACRLSKSAGADFVKTSTGFGPGGATVHDVDLMRHTVGADVGVKASGGVRT
                     FGDLTRMLAAGATRIGASASVKILKEAAEVLGHARRE"
     gene            437..1057
                     /locus_tag="VF720_13610"
     CDS             437..1057
                     /locus_tag="VF720_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017661798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880445.1"
                     /translation="MELRSYVFLDNLQPQHAAFLGTIAQGFLPLAGMASLWVEISPGI
                     EINRVTDIAVKSTRVKPGMQVVERLFGLLEVHHEEQGEVRQAGQAILDAIGLKEDDRL
                     KPRILSSQVIRKIDDHQAQLINRMRHGNMIIPGHTLYVLEVEPAAYAALAANEAEKAA
                     NINILEVRAVGSFGRIYLGGEERDIDVGYRAAVAALESITGRDTKK"
     gene            1106..1414
                     /locus_tag="VF720_13615"
     CDS             1106..1414
                     /locus_tag="VF720_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX7880446.1"
                     /translation="MTDALGMIETRGFAAMVEAADAMVKAAKVEIVGYEKIGGGYVTA
                     IVRGDVAAVKAAVEAGARGAERVGEVVATHVIARPHGNVDTSLPLGRAKEAKAAGARD
                     "
     gene            1424..1732
                     /locus_tag="VF720_13620"
     CDS             1424..1732
                     /locus_tag="VF720_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX7880447.1"
                     /translation="MVLARVLGTVVASRKAEGAMGWKLLLVSALDDTGKPVGGPFVAT
                     DVVGAGESEIVLVASGSSARQTTATDQKPCDAVVMAIVDSWDVNNEIRWRKGRDGTGD
                     "
     gene            1725..>2453
                     /locus_tag="VF720_13625"
     CDS             1725..>2453
                     /locus_tag="VF720_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX7880448.1"
                     /translation="MTDREVQELARRVLQQLAAAGGAVPSTPSGGGGGYGGGSAEPAA
                     RAAASSGGEKLGAFETVDEAVAAANAAQPAFVALSLDKRNAILSHIRKSMRDNAEMLA
                     WMAHSETGLGRFEDKIQKNMLVTNKTPGTEVLVPRAESGDHGLLLEERAPFGTIGAIT
                     PTTNPTSTIICNTIGMLAAGNSVVFNVHPNAKNVSLTNIHLINEAILAAGGPKNVVTA
                     VMNPTVASAGELMKHKGVRLLVVTG"
BASE COUNT          427 a          803 c          888 g          335 t
ORIGIN      
        1 agatcgacat ggtcatcaac gtcgggctcc tgaaggccgg ccgggacgcc gaggtcgagg
       61 ccgacatccg ggccctggcc gatgcctgcc acgactccgg tgcgcgcctc aaggtgatca
      121 tcgaggcggc gctcctcaac gacgaggaaa aggtgcgtgc ctgccggctg tcgaagtcgg
      181 ccggtgcgga cttcgtcaag acgtcgacgg gcttcggccc gggcggcgcc acggtgcacg
      241 acgtggatct catgcgccat acggtcggcg cggatgtcgg cgtcaaggca tccggcggcg
      301 tgcgcacgtt cggcgatctc acccgcatgc tggcggccgg cgccacgcgc atcggcgcgt
      361 cggccagcgt caagatactc aaggaagcgg ccgaggtgct cggccacgcc cggcgcgaat
      421 aggaggccga cgttccatgg aactgcgctc gtacgtcttt ctcgacaatc tccagccgca
      481 gcacgcggcg ttcctgggca ccatcgccca ggggttcctg ccgctggccg ggatggcctc
      541 gctctgggtg gaaatttccc ccggcatcga gatcaaccgg gtcaccgaca tcgcggtcaa
      601 gtccacgcgc gtgaagcccg gcatgcaggt ggtggaacgc ctcttcggcc tcctcgaggt
      661 gcaccatgag gagcaggggg aggtgcgcca ggccggtcag gccatcctcg acgccatcgg
      721 cttgaaggaa gacgaccgcc tgaagccgcg catcctctcg agccaggtga tccgcaagat
      781 cgacgaccac caggcgcagc tcatcaaccg gatgcgccac ggcaacatga tcatccccgg
      841 gcacacgctc tacgtgctcg aggtggagcc ggcggcctac gcggcgctgg cagccaacga
      901 ggcggagaag gcggccaaca tcaacatcct cgaggtgcgc gcggtcggct cgttcggccg
      961 catctatctc gggggcgagg agcgggacat cgacgtcggc taccgcgcgg cggtggcggc
     1021 gctggaatcg atcacgggac gggacacgaa gaagtaggac gcgcgggccg cgccgagcgc
     1081 gtgccccgat cacaggagga acaagatgac cgatgcactg ggaatgatcg agacccgcgg
     1141 cttcgccgcc atggtcgaag cggccgacgc catggtgaag gcggccaagg tggagatcgt
     1201 ggggtacgag aagatcggcg gcggctacgt caccgccatc gtccgcggtg acgtggccgc
     1261 ggtgaaggcc gccgtcgaag ccggcgcccg cggcgccgag cgcgtgggcg aagtggtggc
     1321 cacgcacgtc atcgcgcgtc cgcacggcaa cgtggatacc tcgctgccgc tgggccgggc
     1381 caaggaagcc aaggccgccg gcgcacggga ctaggcgcgg acgatggtgc tggcccgggt
     1441 gctcggcacc gtcgtggcaa gtcggaaggc cgaaggggcg atggggtgga aactcctcct
     1501 cgtctcggcg ctcgacgaca ccggcaagcc ggtcggcggc cccttcgtgg ccaccgacgt
     1561 ggtcggcgcg ggggagtcgg agatcgtgct ggtggcctcg ggcagctcgg cgcgccagac
     1621 gaccgccacc gaccagaaac cgtgcgatgc ggtggtcatg gccatcgtcg attcatggga
     1681 tgtgaacaac gagattcgct ggcgaaaggg acgcgatgga acgggtgact gatcgcgagg
     1741 tgcaggagct ggccagacgc gtgctccagc aactggcggc ggccggcggc gcggtgccgt
     1801 ccacgccgtc gggtggtggt ggcgggtatg gtggcggttc ggcggagccg gcggcccgcg
     1861 cggcggcctc atcgggtggc gagaagctcg gcgctttcga aacggtggac gaggccgtgg
     1921 cggccgccaa cgcggcccag ccggccttcg tggcgctctc cctcgacaag cggaacgcca
     1981 tcctctcgca catccgcaag tcgatgcgcg acaacgccga gatgctggcc tggatggcgc
     2041 attcggagac gggcctcggc cgcttcgagg acaagatcca gaagaacatg ctcgtcacga
     2101 acaagacgcc cggcaccgag gtcctggtgc cgcgcgccga gtcgggcgac cacgggttgc
     2161 tcctcgagga gcgggcgccg ttcggcacga tcggcgccat cacgccgacc acgaacccca
     2221 cgtccaccat catctgcaac acgatcggga tgctggcggc ggggaactcg gtcgtcttca
     2281 acgtgcaccc caacgcgaag aacgtctcgc tcacgaatat ccacctgatc aacgaggcca
     2341 tcctggccgc cggcggaccg aaaaacgtgg tcaccgcggt catgaacccc acggtggcct
     2401 cggccggcga gctgatgaag cacaaggggg tccggctgct ggtcgtgacc ggc
//
LOCUS       DASVOD010000626         6526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_352908, whole genome shotgun sequence.
ACCESSION   DASVOD010000626 DASVOD010000000
VERSION     DASVOD010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6526
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352908"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VF720_13630"
     CDS             complement(<1..1104)
                     /locus_tag="VF720_13630"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX7880449.1"
                     /translation="MSGVRVLVGTKKGAFVLTSDAKRQKWDVQGPHFAGWELYHLKGS
                     PLNPDRIYASQCSGWFGQMIQRSDDGGKTWAPVDNKFVYDGVPGTHQWYDGTQHPWEF
                     KRVWHLEPSLTEPDTVYAGIEDAAMFKSTDAGASWTELSGLRNHDTGPKWQPGAGGMC
                     LHTILIDPTNPKRIFIAISAAGAFRTDDGGITWKPINKGLSSQYIPDPNAEVGHCVHR
                     IASHPSKPDTLFMQKHWDIMRSDNGGDSWTEVSGNLPTDFGFPIDVHAHEPETIYVVP
                     IKSDSEHYPLDGKLQVWRSRSGGNEWEPLTKGLPQKDCYVNILRDAMSVDRLDSCGIY
                     FGTTGGQVYCSSDSGDSWNAIVHDLPAVLSVEVQ"
     gene            complement(1219..2685)
                     /locus_tag="VF720_13635"
     CDS             complement(1219..2685)
                     /locus_tag="VF720_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880450.1"
                     /translation="MSAGHVVVSLAPLRPALILAMALLVSSLGCGGGGEPSVVEVEID
                     CPLSPKAPGTAFTLIARENMSTTHQWSVSSTTTAFRLTSDTTSVQSTFSLGEHQALLT
                     VKTAPDPAQTSIYYSQIEVTAAQVGGLDVRVTDKERVDADICAVVIGSTGYVATDIHA
                     DVEAAIGSSAPRGLQPLLLGESQALGANDAGEVVGFIETSTGNRAFLWKNGFVVDLGP
                     GSANAISSDGWITGSRPVTVPRPPDPDVIVDHAFLWQPTVPNGTTGTFIDLGTLVNPA
                     TNPFAGSYGSAINDNHDVAGTSDAEIGQQAFVTRNGVMVNIGAHAGATFSFAEGINNR
                     GDVCGEVLGSVVQAALWPASGGVLDLHDETQVLESFATGINDSGFVVITGHTDQGSNL
                     GFLWRSGSPLARLLIVSSSLSVAPEAINTAGAIVAEDSHNLFGFLASAPTAGNTILIS
                     NLTGEILRVRPNDLTDGGLIAGHFVRPTGTEAVLLMPQ"
     gene            2881..4887
                     /locus_tag="VF720_13640"
     CDS             2881..4887
                     /locus_tag="VF720_13640"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011370491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEX7880451.1"
                     /translation="MERPMLEDRRKLLETELRAHGVRDAGVMAAMKNVPRQEFVPDEL
                     REFAWRNTALPIESGQTISQPSMVAVMCQALELSPRDRVLEIGTGSGYAAAILSRLAA
                     EVFTVERHWDLAQAAAERLKRLGYLNVHIRHADGTRGWPEQAPFDAIVVAAGGPGVPK
                     PLLEQLAGGGRLVIPVGSTRDSQKLLRITRRGVDRYDREWLGDVAFVPLIGEAGWQDD
                     VPLAPTPDVPVDGREAGVIDAIRRAAEPFGDPDELDAARLLERIGDARIVLIGEASHG
                     TSEFYRVRAAITKALIAAGRCDFVAVEADWPDAMRIHRYVTNRRLAEPPLRDAFARFP
                     TWMWRNHETQDFIHWLRDVNAEGRTNQPIGFYGLDLYSLSASIEAVLDYLDKVDPTAA
                     GVARRRYGCLTPWENDPAMYGLAARSPGFATCEPQVVSMLTEMLGRRVETSHADHEEL
                     FDARVNARLVADAERYYRLMYAGGAASWNWRDTHMFETLRELLDRHGSASRGVVWAHN
                     SHLGDATATQFAREGQHNIGQLCRELFGDEMYAIGQGTDSGTVAAASDWDGPLEIKSV
                     APAHVDSYERLMHRAELGAFLLPLGRRVATRLTEVLTPVRLERAIGVIYRPDTERWSH
                     YFEASLPRQFDEWIWFDRSTAVRPLGPGAVAGHESEHPFALRDT"
     gene            5121..6455
                     /locus_tag="VF720_13645"
     CDS             5121..6455
                     /locus_tag="VF720_13645"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HEX7880452.1"
                     /translation="MRSPKYVAALLVVLALAVSVSLVRSRPDSGPELLAGNGWTKLPN
                     GPNAAGAQRHDDLCFIDEYRGWVVNGNGEVWRTSDGGNSWTRTALTGDYNRCVGFINQ
                     NTGWIGYLYQVNGTALIRTDDGGSTWNSVTLPAPAPAGVCGMQVIDSTTVVAVGAYYG
                     TPTFIRTDDAGANWSVLDMSPYCGALVDVHFFNDQVGVAVGSSTPGPNRRHMILRTTD
                     GGITWEVRQVGSRNRELCWKASFPNEQAGFVSIENLAGIGPTYFLKTQDGGVNWTEVF
                     ATSSFVDCQGIGFVTPYFGWIGGWNTGTAVTKDGGNSWAGGDFGFNVNRIRFLSPFLG
                     YACGTDVYKFQLNPAGVGNGDVASGPPLVQVRPNPFSSTTRISYRVPKEGPISLRVFD
                     VQGRPIRALIDGESAAGDFTTPFDAHGLAAGTYYYRLSTVAGSETGKLQVVP"
BASE COUNT         1109 a         2187 c         2126 g         1104 t
ORIGIN      
        1 ctgaacctcc accgacagga ccgccggcag atcgtgcacg atggcgttcc acgagtcgcc
       61 cgaatcggac gagcaataca cctgcccgcc ggtcgtcccg aagtagatgc cgcacgaatc
      121 gagccggtcc accgacatgg catcgcgcag aatgttcacg tagcagtcct tctgcgggag
      181 ccccttcgtg agcggctccc actcgttccc gcccgatctg ctgcgccaca cctgcaactt
      241 gccgtccagc ggatagtgct cggaatcgct cttgatcggc accacataga tggtttcggg
      301 ctcgtgcgcg tgcacgtcga tcggaaaacc gaagtcggtc ggcaggttgc cgctcacctc
      361 ggtccacgaa tcgccgccgt tgtcggaacg catgatgtcc caatgcttct gcatgaagag
      421 cgtgtccggc ttcgagggat gggacgcgat gcgatgcacg cagtgcccca cctccgcgtt
      481 gggatcggga atgtactggg atgacagccc cttgttgatc ggcttccagg tgatgccgcc
      541 atcgtcggta cggaaggctc ccgccgccga gatggcgatg aagatccgtt tcgggttggt
      601 cgggtcgatc aggatggtgt gcaggcacat gccacctgcg cccggctgcc atttggggcc
      661 cgtgtcgtgg ttgcgcaggc ccgagagctc cgtccaggag gcgccggcgt cggtggactt
      721 gaacatcgcc gcatcctcga tcccggcata taccgtgtcg ggctcggtca ggctcggctc
      781 gaggtgccac acccgcttga actcccacgg atgctgcgtg ccgtcgtacc actggtgcgt
      841 gccagggacg ccgtcataga cgaacttgtt gtctacgggg gcccacgtct tcccgccgtc
      901 atcggagcgc tggatcatct gcccgaacca gccgctgcac tgcgaggcat agatccggtc
      961 cgggttcagc ggcgagccct tcaggtgata cagctcccac cccgcgaaat gcggcccctg
     1021 cacatcccat ttctggcgtt tcgcgtccga cgtgaggacg aacgccccct ttttcgtgcc
     1081 aacgagtaca cggactccac tcatgtcctg ctccttctga accccgcgcc cggggtgaac
     1141 ggttcgcagc cgaaaggaca ttgatcctag caaaagggag tgggcccggc gcgggagcag
     1201 gatcgcgagg cagccgacct actgcggcat gagcagcacc gcctccgtcc ccgtcggccg
     1261 cacgaaatga cccgcgatca gcccgccatc cgtcaggtcg ttcggccgga cacggagtat
     1321 ttcgccggtc aggttcgaga tgagaatggt attgccggcc gtcggcgcgg aggccaggaa
     1381 gccgaacagg ttgtggctgt cctcggccac gatggcaccc gccgtgttga tcgcctcggg
     1441 ggccaccgac aggctggagc tcacgatgag aaggcgtgcg aggggtgagc ccgagcgcca
     1501 caggaatccc aggttggatc cctggtccgt gtgcccggtg atgacgacga accccgagtc
     1561 gttgatgccg gtggcaaaac tttccaacac ctgcgtttcg tcgtgaaggt cgagcacgcc
     1621 gcccgacgcc ggccacaacg ccgcctgcac cacgctcccc agcacctcgc cgcagacgtc
     1681 gccgcgatta ttgatcccct ccgcaaacga gaaggttgcg ccggcatgcg cgccgatgtt
     1741 gaccatgacg ccgttccggg tcacgaaggc ctgctggccg atctcggcgt cggaagtccc
     1801 cgccacatcg tgattgtcgt tgatggcgct tccgtaactg ccggcgaacg gattggtggc
     1861 cggattgacg agagtgccga ggtcgatgaa cgttcccgtc gtgccgttgg gcaccgtcgg
     1921 ctgccagagg aacgcatgat ccacgatcac gtccggatcg ggcgggcgcg gcaccgtgac
     1981 cggacgggaa ccggtgatcc atccatccga gctgatggcg ttggcggatc cggggcccag
     2041 gtcaaccacg aaaccgttct tccagaggaa ggcccggttg ccggtcgacg tttcgatgaa
     2101 ccccaccacc tcgcccgcgt cgttcgcgcc cagggcctgt gattcgccga gcaggagcgg
     2161 ttgcaatcca cgcggcgcgg agctcccgat ggccgcttcc acgtcggcgt ggatgtcggt
     2221 ggccacgtag ccggtcgatc cgatgaccac ggcgcagatg tcggcgtcga cccgttcctt
     2281 gtcggtaacc cggacgtcga gccccccgac ctgcgcggcg gtgacctcga tctgcgagta
     2341 atagatcgat gtctgcgcgg ggtctggtgc ggttttgact gtcagcaagg cctgatgctc
     2401 acccaggctg aacgtcgact gtaccgaggt cgtatccgag gtgaggcgaa atgcggtggt
     2461 ggtgctgctg acggaccact gatgcgtggt gctcatgttt tctcgagcga tgagcgtgaa
     2521 ggcggttccg ggcgccttcg gtgagagggg gcaatcgatc tcgacctcga ccacgctcgg
     2581 ctcgcctccg cctccgcagc cgagcgacga gacgagcagg gccatggcca ggatgagtgc
     2641 cggacggagc ggggcaagcg aaacaacaac atgaccagcg gacatggcgg cctccgatgg
     2701 ttggtgtcgg cgctcacctt atataggaac gccggaattc acgagagtcg ggagactacc
     2761 agtaaatatt tcctcatgtt ggtggtgtca ttgtcatcgc gtgttccaac acccatgccc
     2821 tccggcacct ttgatgcatc cactcccgtg ccgccactct ggccgggaag gagttgcctc
     2881 atggaacgcc ccatgctcga agaccgacgc aagctcctgg aaaccgaact gcgcgcgcac
     2941 ggagtgcggg acgccggcgt gatggccgcc atgaagaacg tgccgcggca ggagttcgtg
     3001 cccgacgagc tgcgcgagtt tgcgtggcgg aacacggcgc tgccgatcga gtcggggcag
     3061 acgatctccc agccgtccat ggtggcggtg atgtgccagg cgctcgagtt gtcgcctcgc
     3121 gatcgcgtcc tcgagatcgg aacggggtcg ggatatgccg cggcgatcct gtcgcggctg
     3181 gcggccgaag tgttcaccgt cgagcggcat tgggacctgg cgcaggcggc ggcggagcgg
     3241 ctcaagcgcc tcggttacct gaacgtgcac atccggcacg cggacggcac gcggggctgg
     3301 cccgagcagg cgccgttcga tgccatcgtg gtggcggccg gcgggccggg cgtgccgaag
     3361 ccgctgctcg agcagctggc cggtggcggg cggctggtca ttccggtcgg ttcgactcgc
     3421 gatagccaga agctgctccg catcacgcgg cgcggtgtcg atcgctacga cagggaatgg
     3481 ctgggcgacg tggccttcgt gccgctgatc ggcgaggcgg gctggcagga cgacgtgccg
     3541 ctggcgccaa cacccgacgt gccggtggac gggcgcgaag ccggcgtcat cgacgccatc
     3601 cgccgcgccg ccgagccctt cggtgatccg gacgagctcg acgccgcgcg cctcctcgag
     3661 cggatcggtg acgcgcgaat cgtgctgatc ggcgaggcct cgcacgggac gtccgagttc
     3721 taccgggtgc gcgcggccat cacgaaggcg ctcatcgccg ccgggcggtg cgacttcgtg
     3781 gccgtggagg ccgactggcc ggatgccatg cgcatccacc gttatgtcac gaatcgacga
     3841 ctggccgagc cgccgctccg cgacgccttc gcgcgcttcc ccacctggat gtggcgcaac
     3901 cacgaaacgc aggacttcat ccattggctg cgcgacgtga acgctgaagg tcgcaccaat
     3961 caacccattg gcttctacgg cctggatttg tacagcctgt ccgcctccat cgaggcagta
     4021 ctcgactatc tcgacaaggt ggatcccacg gcggcgggtg tggcgcggcg gcggtacggc
     4081 tgcctcacgc cgtgggaaaa cgacccggcg atgtacggac tcgccgcccg atcgcccggc
     4141 ttcgccacct gcgagccgca ggtcgtctcg atgctcaccg agatgctcgg gcgccgcgtg
     4201 gaaacgtcgc atgccgacca cgaagagctg ttcgatgccc gggtcaacgc ccgcctggtg
     4261 gcggatgccg agcgctacta ccgcctgatg tacgccggcg gcgcggcctc ctggaattgg
     4321 cgcgacaccc acatgttcga aacgctgcgc gagctgctcg atcggcacgg ctccgcgtcg
     4381 cgcggcgtgg tgtgggcgca caactcccat ctgggagatg ccacggccac gcagttcgcc
     4441 cgcgaggggc agcacaacat cggccagctc tgccgcgagc tgttcggcga cgagatgtac
     4501 gccatcggcc agggaactga cagcggcacc gtggccgccg ccagcgactg ggacgggcct
     4561 ctcgagatca agagcgtggc gcccgcgcac gtggacagtt acgagcggtt gatgcaccgc
     4621 gccgagctgg gcgccttcct cctgccgctg ggacgccgcg tcgccacgcg gctcaccgaa
     4681 gtgctcacac cggtgcggct cgagcgggcc atcggcgtca tctaccggcc ggataccgaa
     4741 cgctggagcc actatttcga ggcgtcgctc ccccgccagt tcgacgaatg gatctggttc
     4801 gaccggagca cggccgtccg gccgctgggg cccggggccg tggcgggaca tgagtcggag
     4861 cacccgttcg ccctgcggga cacatgacga caaggagctg atcgcatgta accacaataa
     4921 gttaacaagg attgccaagc cggtcgggag gcccggggcc tgttgattcc gcgcggcgga
     4981 cccacccccg tcgtgtacaa tcgtccgact cattccggcc gaagtaactt ttcaggccgc
     5041 caacacggag gccggaatgc cgtcaccgcc gccaaggcga attcccgtca ttacataacc
     5101 gaaaggttcg aaccgcgttc atgcgatcgc cgaagtacgt tgccgcgctc ctcgtcgtgc
     5161 tggccctggc cgtcagcgtt tcgctcgtcc gttcccgccc cgactccggt cccgagctcc
     5221 tcgccggcaa cggctggacc aagcttccca acggaccgaa cgccgccggc gcgcagcgtc
     5281 acgacgacct ctgtttcatc gatgagtatc gcggctgggt ggtcaacggc aatggcgaag
     5341 tgtggcgcac gtcggacggc ggcaattcgt ggacgcgcac cgcgctcacc ggcgattaca
     5401 accgctgcgt cggcttcatc aaccagaaca ccggctggat cggctacctc tatcaggtga
     5461 acggcaccgc cctcatccgc accgacgacg gcggctcgac ctggaactcc gtcacgctcc
     5521 ccgcaccggc acccgcgggc gtctgcggca tgcaggtcat cgactcgacg accgtcgtgg
     5581 cagtcggcgc ctactacgga actcccacgt tcatccgcac cgacgatgcc ggcgccaact
     5641 ggagcgtgct cgacatgtcg ccttactgcg gcgcgctggt cgacgtccac ttcttcaatg
     5701 accaggtggg cgtggccgtc ggctcctcca cgccgggccc caaccgtcgc cacatgatcc
     5761 ttcgcacgac cgacggcggc atcacctggg aagtgcgtca ggtcggttct cgcaaccgcg
     5821 agctctgctg gaaggcgtcg ttccccaacg aacaggccgg cttcgtctcc atcgagaacc
     5881 tggccggcat cggccccacc tacttcctga agacccagga cgggggcgtc aactggacgg
     5941 aagttttcgc cacctccagc ttcgtcgact gccaggggat cggcttcgtg acaccctact
     6001 tcggctggat cggcggctgg aacaccggca ctgccgtcac caaggacggc ggcaattcat
     6061 gggcgggcgg agacttcggc ttcaatgtca accgcatccg cttcctctcg cccttcctcg
     6121 gctacgcctg cggcaccgac gtctacaagt tccagttgaa ccccgccggc gtcggcaatg
     6181 gcgacgtggc ctccggccct ccgctcgtgc aggtgcgccc gaatccattc tcctccacca
     6241 cgcgcatctc ctaccgcgtg ccgaaggaag gacccatctc gctgcgcgtg ttcgacgtgc
     6301 aggggcgccc catccgcgcc ctcatcgacg gcgaatcggc cgccggtgat ttcacgacac
     6361 cgttcgacgc acacggactc gcggcaggga cgtactacta ccggttgagc acggtcgcgg
     6421 gaagcgagac agggaagctg caggtggtgc cgtagccgaa ctagatacgt tcaaggggag
     6481 gacagggaag aattgtccga aatgggcaac ccgaacaaga ctggga
//
LOCUS       DASVOD010000627         1565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_43932, whole genome shotgun sequence.
ACCESSION   DASVOD010000627 DASVOD010000000
VERSION     DASVOD010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43932"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..>1565)
                     /locus_tag="VF720_13650"
     CDS             complement(94..>1565)
                     /locus_tag="VF720_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880453.1"
                     /translation="RRREQDLRDGIEGTDARFSIGVTDPEGLYRLTIWLADTLRVTGP
                     VAIDIGDMQVASDIEGIAGKRRALRWLVRPGHDRVSFRIMADPCDRFGVTAARLEGPP
                     GAGLFAIFPGIDTSAAEFPPPEALPLITESALREQLRRDAEHLMSERLGDGRFSTHGA
                     WYENSFPIRALVAAGEILGEPAWKEAAFAVLDDFVSRQRPDGNWVSDYEGDEACPRKG
                     TSIVSANLADIGTMTLALAIAADDADPVRRDRWVRALVTYADSVSLPNQDSTGGFCNR
                     TWQNRDYRFPYTVATATQVSTLAALHRHTGNPRYAEAVARGTRWLADQVLDDGRIAFA
                     PHDRDAVKLLDATHFGDCYYAMETLALVGVWTRDEALRHQADAAFDRWVGGRQGLRAV
                     AREGYWWPMGDLWADSKMAGVPALLARRARRPSPPWLREMVLRQLAWLDDDARARRIG
                     VRCSFASQRGEFALAATGFAALSASSALRMRPGLAGIVE"
BASE COUNT          247 a          540 c          549 g          229 t
ORIGIN      
        1 tggcctccgg tttctgtgcg gtgtcgttcg gcaccgcggc ggccgtgccc gggccgaacc
       61 cgatcaacgc gagggcgagg aggagggcgc gcgtcattcc acgatcccgg ccaatccggg
      121 ccgcatccgg agcgccgacg acgccgagag ggccgcgaac ccggtagcgg ccagggcaaa
      181 ctcgccgcgc tgcgaggcaa agctgcaacg cacgccgatc cggcgagccc gcgcgtcgtc
      241 gtcgagccag gccagctgcc gaagcaccat ttcgcgaagc cacggaggtg aggggcgccg
      301 cgcccggcgg gccaggagtg ccgggacgcc ggccattttc gagtcggccc agagatcgcc
      361 catcggccac cagtaccctt cacgcgccac cgctcggagc ccctgcctgc cgcccaccca
      421 gcgatcgaag gcggcatcgg cctggtgccg gagggcctcg tcccttgtcc agacccccac
      481 gagggccagc gtctccatcg cgtaatagca gtcgccgaag tgggtggcat cgagcagctt
      541 caccgcatcg cgatcatgcg gggcgaaggc gattcgaccg tcgtccagca cctggtcggc
      601 aagccagcgc gtcccgcgcg ccaccgcctc ggcatagcgg ggattgccgg tgtggcgatg
      661 gagggcggcc aaggtcgaca cctgcgttgc ggtggccacg gtatacggaa accggtagtc
      721 ccggttctgc caggtgcggt tgcagaagcc gccggtcgaa tcctggttgg ggagagagac
      781 cgagtcggcg taggtgacga gtgccctcac ccatcgatcg cggcgcaccg gatcggcatc
      841 gtcggccgcg atggccaggg ccagcgtcat cgtgccgatg tcggccaggt tggcgctgac
      901 gatggatgtc cctttccgcg ggcacgcctc gtccccttcg taatcgctga cccagttgcc
      961 gtccgggcgc tggcgggaaa cgaagtcgtc gagaaccgca aaggccgcct ccttccaggc
     1021 cggttcgccc aggatctccc cggcggccac gagcgcccgg atggggaagg agttctcgta
     1081 ccaggccccg tgcgtgctga acctgccgtc gccgagccgt tcgctcatga gatgttcggc
     1141 gtcccgccgg agctgttctc gaagtgccga ttccgtgatc aaggggagcg cttccggggg
     1201 cgggaattcg gccgccgacg tgtcgatgcc cggaaagatg gcgaagaggc cggcgccggg
     1261 gggtccctcg agacgcgcgg cggtcacgcc gaaccggtcg cagggatcgg ccatgatccg
     1321 gaaactcacg cggtcatggc ccggacgcac cagccagcga agggcgcgcc gctttccggc
     1381 aattccttcg atgtcggaag ccacctgcat gtcgccgata tcgatcgcca cggggccggt
     1441 cacgcggagt gtgtcggcca gccagatcgt gagccgataa agtccctcgg gatccgtgac
     1501 tccgatcgag aagcgcgcat ccgtcccctc gatgccgtcg cgcaggtcct gctcccggcg
     1561 gcgcg
//
LOCUS       DASVOD010000628         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_927108, whole genome shotgun sequence.
ACCESSION   DASVOD010000628 DASVOD010000000
VERSION     DASVOD010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927108"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF720_13655"
     CDS             complement(<1..369)
                     /locus_tag="VF720_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEX7880454.1"
                     /translation="MTSTADSAGAADPTGPAVVVLLGTTASGKTAVSIPLARALGAEI
                     LALDSKQVLAGMPIGTAQPTDAEREDIPHHLFGVVDPRERFSAGDYGRLARQALAVIE
                     ARRGRALFVGGSGLYLRALLG"
     gene            complement(335..2149)
                     /gene="mutL"
                     /locus_tag="VF720_13660"
     CDS             complement(335..2149)
                     /gene="mutL"
                     /locus_tag="VF720_13660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEX7880455.1"
                     /translation="MTKVALPVIRRLEGDVVARIAAGEVIDRPAAVVKELFENALDAG
                     AHRITVEVDGHLERMVRVSDDGHGMDEAGVMLAIERHATSKIERAEDLSRVLSLGFRG
                     EGLASVAAVSRLTILSAGQDGDGVEMVSEGGHISSMRPAGRARGTTIEVRDLFFNTPV
                     RRRFQRAATTELQHSARLIQSYALARRDVHLKWIVDGREILNLPPSGSLAERMTALFG
                     RERVARMIPVEARNDVVAVEGFLGAPEDSRARGDQQTFFVNGRLVTNPVLRQAVRQAY
                     GNLIPYERHPFVVLSVTIDPARVDVNIHPTKKEVRFSNDRDLFPALVSLVGPAVLKHV
                     PRFRPGDAGDGLAVDDMASLFGGGGLALDAPPADPPVGSAGPSAPGDTPSLGAAPGTP
                     TILPFAGTLTGGTAFKDQSEAGEAVLADLWQLHRSYILASIRGGLLVIDQHAAHERVL
                     FEEAILRIRSGRPATQELLFPQVVPLTAEEFHVLLDVQPTLEKFGFHVELFGGTTVVV
                     HGIPAGLTRWRQGEMLHDLIAMYGDQPAGAPLEERVARSYACRAAVKAGEPLTQVEMN
                     ELIDRLFATTLPQGDPHGRPTFLRLSIDDIHRRFGRSS"
     gene            complement(2146..>2416)
                     /locus_tag="VF720_13665"
     CDS             complement(2146..>2416)
                     /locus_tag="VF720_13665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880456.1"
                     /translation="HLSVPVGSGLIGGAFGGTTRASWFCEAADGSGEIVTIPVDGGRG
                     GQRAATGVLLIEGVPPADDDPRDAERRVDLLRDILAPLVPPVSAG"
BASE COUNT          371 a          837 c          879 g          329 t
ORIGIN      
        1 accaaggagg gcacgcaggt agagacccga gccgccgacg aagagcgctc ggccgcggcg
       61 cgcctcgatg accgccagcg cctggcgcgc cagtcgacca tagtcacccg cgctgaagcg
      121 ctcgcgcgga tcgaccacgc cgaagagatg atggggaatg tcctcgcgct cggcgtcggt
      181 cggctgggcc gtgccgatcg gcatgccggc cagcacctgc ttcgaatcga gcgccagtat
      241 ttccgcgccg agcgcacggg ccagcgggat ggacaccgcg gtcttccccg acgcggtggt
      301 gccgaggagg acgacgaccg cgggacccgt cgggtcagct gctccggccg aatcggcggt
      361 ggatgtcatc gatcgacagc ctgaggaacg tggggcgccc gtggggatcg ccctgcggca
      421 gcgtggtggc gaagaggcgg tcgatcagct cgttcatctc gacctgcgtg agcggttccc
      481 ccgccttgac cgcggcgcgg caggcatacg agcgggccac gcgctcctcg agcggcgcgc
      541 ccgcgggttg gtcgccatac atggcgatga ggtcgtgaag catctccccc tggcgccagc
      601 gggtgaggcc ggcggggatg ccgtgcacca ccaccgtcgt gccgccgaac agctccacgt
      661 ggaagccgaa cttctcgagc gtaggctgca cgtcgagcag cacgtggaac tcctcggccg
      721 tcagcggcac cacctgcggg aagaggagtt cctgcgtggc ggggcggccg ctgcggatgc
      781 ggaggatggc ctcctcgaag agcacgcgct cgtgggcggc gtgctggtcg atgacgagga
      841 gcccgccccg gatggaagcc aggatgtagc tccggtggag ctgccagaga tcggccagga
      901 cggcctcccc cgcttccgac tgatccttga aggccgtgcc gccggtaagc gtgccggcga
      961 acggcaggat ggtgggggtg cctggtgcgg cgcccaggga cggagtgtca cccggggccg
     1021 atggtccggc actacccacc ggcggatcag cgggcggtgc gtcgagcgcc agcccgccac
     1081 cgccgaagag gctggccatg tcatcgaccg ccagcccgtc gccggcatcc cccgggcgga
     1141 agcgcggcac atgcttcagc accgcgggcc ccaccagaga gacgagcgcc gggaagaggt
     1201 cgcggtcgtt cgagaagcgc acttccttct tcgtggggtg gatgttcacg tccacccggg
     1261 ccgggtcgat cgtgaccgac aggacgacga acgggtgccg ctcgtacggg atcaggttgc
     1321 cgtaagcctg tcgcaccgcc tggcgaagca ccgggttcgt gacgaggcgg ccgttgacga
     1381 agaaagtctg ctgatctccc cgggcccgcg aatcctcggg cgcgccgaga aatccttcga
     1441 ccgccaccac gtcgttccgc gcctcgaccg ggatcatccg ggccacgcgc tcgcgcccga
     1501 agagggccgt catccgctcg gcaagggaac cgcttggcgg gaggttcagg atctcgcggc
     1561 cgtcgacgat ccacttcagg tgaacgtcgc gccgcgccag cgcgtacgac tggatgagcc
     1621 gcgcggaatg ctgcagctcg gtggtggcgg cccgctggaa tcgccggcgc accggcgtat
     1681 tgaagaagag gtcgcgcacc tcgatggtgg tgccgcgggc gcgcccggcc ggccgcatcg
     1741 agctgatatg ccccccttcg gacaccattt ccacgccgtc gccgtcctgg ccggccgaga
     1801 ggatcgtgag gcgcgagacg gccgccaccg aggccagccc ttcgccgcgg aagccgagcg
     1861 acaggacgcg cgaaaggtcc tcggcccgct cgatcttgct ggtggcgtgc ctctcgatgg
     1921 ccagcatcac gccggcttca tccatgccgt ggccgtcgtc gctcacgcgg accatccgtt
     1981 cgagatggcc atccacctcc acggtgatgc ggtgagcacc ggcatcgagc gcgttctcga
     2041 agagttcctt gacgacggcg gccggccggt cgatgacctc gccggccgcg atccgggcca
     2101 ccacgtctcc ttcgaggcga cggatgaccg gcagggcgac cttcgtcatc cggccgatac
     2161 gggcgggacg agcggggcca ggatgtcgcg caggagatcg acgcggcgct cggcgtcgcg
     2221 cgggtcgtcg tcggcggggg gcaccccctc gatcaggagg acgccggtgg ccgcgcgctg
     2281 cccgccgcgg ccgccatcca ccgggatggt gacgatctcg cccgagccgt cggccgcctc
     2341 gcagaaccag ctggcccgcg tggtgccgcc gaaggcgccg ccgatgagcc ccgaaccgac
     2401 cggcaccgag agatgc
//
LOCUS       DASVOD010000629         1869 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_302374, whole genome shotgun sequence.
ACCESSION   DASVOD010000629 DASVOD010000000
VERSION     DASVOD010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302374"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF720_13670"
     CDS             complement(<1..492)
                     /locus_tag="VF720_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880457.1"
                     /translation="MPSYPMERHVPIIDLTVPPGLKKPVEKLLKCPVMGDDRKAIEAL
                     TAEESPRRRMPVLSEAFRNYETREMVQLLRTAGKMSLEHDPVGLRLLFSFLEVKRPGV
                     EVLRQLSTLASLERVSLKVLLGTWRDADAGVTDAAFPILELMREAGRSGVLDLGGDAL
                     PKDP"
     gene            complement(573..1031)
                     /gene="bcp"
                     /locus_tag="VF720_13675"
     CDS             complement(573..1031)
                     /gene="bcp"
                     /locus_tag="VF720_13675"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014103598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(894..896),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX7880458.1"
                     /translation="MDMLKEGQKAPAFSLPADDGTTVSLADFKGKKLILFFYPKDMTS
                     GUTIEARGFRDELARIRKRGAEVVGVSKDSVKSHVKFREKEGLNYRLLSDENGDICQR
                     YGVWVEKSMYGKKYMGIARTTFLIDEAGRIEKIFEKVAVEGHCGEVMESL"
     gene            complement(1075..>1869)
                     /locus_tag="VF720_13680"
     CDS             complement(1075..>1869)
                     /locus_tag="VF720_13680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEX7880459.1"
                     /translation="KVRLAIEPTTWFWVAEKIRAALPTARLVDAGPLLDRMRSMKSVE
                     EIDRIRRAGDLAEAAVHVARLHLREGMSELEAAEILIDAVKTEDGFHEPLVQFGPNSA
                     IPHATAGSRRLERGQMVLFDLSGVVGGYLSDITRMTTFGDATVEMKKVYDIVHEAQQA
                     AIAVAGPGVACQEVDRAARQVITRAGYGEFFTHRTGHGLGLDIHEPPFLVEGNATPLE
                     PGQVVTIEPGIYLPGKFGVRIEDDIVITADGAEVLTDGQPQLVETP"
BASE COUNT          282 a          622 c          631 g          334 t
ORIGIN      
        1 ggggtccttg ggcagggcat cgccgccgag atcgagcacg cccgagcgcc cggcctcgcg
       61 catcagttcg aggatcggga aggccgcgtc ggtgacgccg gcatcggcgt cacgccaggt
      121 gccgagcagg accttgagcg acacccgctc gaggctggcc agcgtgctga gctggcggag
      181 cacttccact cccggccgct tcacctcgag gaacgaaaag aggaggcgaa gcccgacggg
      241 gtcgtgctcc agcgacatct tgccggccgt gcgcaggagc tggaccatct cccgcgtttc
      301 gtagttgcgg aaggcctcgg aaaggaccgg catgcgccgg cgcggagact cctcggcggt
      361 cagcgcctcg atcgccttcc ggtcgtctcc catcaccggg cacttgagaa gcttctcgac
      421 cggcttcttc aagccgggcg gaacggtgag atcgatgatc ggaacgtgcc gttccattgg
      481 gtagctgggc atcgatcccg ttatcggccg ggctgggaac gacttaaggc cgatccgggg
      541 cttttggcgc ggcgggaggg cctcgggaag ggttacaggc tttccatgac ctcgccgcag
      601 tgcccctcga cggccacctt ctcgaagatc ttctcgatcc gccccgcctc gtcgatcagg
      661 aacgtggtgc gggcgatccc catgtacttc ttcccgtaca tcgatttctc gacccagacg
      721 ccgtagcgct ggcagatatc gccgttctcg tcggagagga ggcgatagtt gagcccttcc
      781 ttctcgcgga acttgacgtg cgacttgacc gagtccttgc tcacgccgac gacctcggcg
      841 ccccgcttcc ggatccgggc cagctcgtcc cggaacccgc gggcctcgat cgttcagccg
      901 gaggtcatgt ccttggggta gaagaagagg atcagcttct tccccttgaa gtcggcgagc
      961 gacacggtcg tgccgtcgtc ggcgggaagc gagaaggcgg gcgccttctg tccttccttc
     1021 agcatgtcga tcctttccgc gttcgcgtgc cgatccgggg caatccggga tcggtcaggg
     1081 cgtttccacg agttgcggct ggccgtcggt gagcacctcg gcgccatccg ccgtaatcac
     1141 gatgtcgtcc tcgatgcgca cgccgaactt gccgggaagg tagatccccg gctcgatcgt
     1201 gaccacctgt cccggctcga gcggcgtggc gtttccctcc acgaggaacg gtggctcgtg
     1261 gatgtcgagc ccgaggccgt gtccggtgcg gtgggtgaag aattcgccat agcccgcccg
     1321 ggtgatcacc tggcgggccg cgcggtcgac ttcctggcag gccacgccgg gccccgccac
     1381 ggcgatggcc gcctgctgcg cttcatgcac gatgtcgtac accttcttca tttcgacggt
     1441 ggcgtcgccg aaggtggtca tgcgggtgat gtccgacagg tagcctccca ccacgcccga
     1501 caggtcgaac aggaccatct ggcccctttc cagacggcgc gaaccggcgg tggcgtgggg
     1561 gatggccgag ttcggcccga actgcacgag cggctcgtgg aagccgtcct cggtcttcac
     1621 ggcatcgatg aggatctcgg ccgcctcgag ctcgctcatc ccctcgcgga gatggagacg
     1681 cgccacgtgg acggcggcct cggccaggtc gcccgcccga cggatgcgat cgatctcctc
     1741 gaccgacttc atcgagcgca tgcggtcgag cagcgggccg gcgtcgacga ggcgtgccgt
     1801 ggggagggcc gcccggatct tttcggccac ccagaaccag gtggtcggct cgatggcgag
     1861 gcgcacctt
//
LOCUS       DASVOD010000630         3059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_302442, whole genome shotgun sequence.
ACCESSION   DASVOD010000630 DASVOD010000000
VERSION     DASVOD010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302442"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="VF720_13685"
     CDS             <1..1079
                     /locus_tag="VF720_13685"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880460.1"
                     /translation="VYVAEPVNGRFLQFTTAGSVVATWQGVGRDVARGVGDDLFVIDN
                     YVTRIRRFTAREIDCGPPAPLPNGAPGIAIGVHVTEAGSPGPCLSGVTFDCTQSVTRG
                     GLASPDIGPWYYVYVLAKVDTMLEVGGLQCGVMYQGGSPSGLQDQVGLDVYSWTSCAD
                     FQFADPAWPAPGTGNLIAWDPDSRCQRTQSAIAGFFYVGAYTPDALSITAHPATGVAT
                     LVDCHFQHRPVHPQRLGWAPFSPGVDESGCNPCRSIRCAPPTSGVPGVQALSSGPPVP
                     NPFRSSVSFRYTLPAPATVEAAIFDIQGRRIRALPAVRQPVGEHEFRWDGRDDAGHDA
                     ASGAYFARVVAGSRVLEKRLLRIP"
     gene            1171..2859
                     /locus_tag="VF720_13690"
     CDS             1171..2859
                     /locus_tag="VF720_13690"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7880461.1"
                     /translation="MVPAAFALLAVLAPVPDVSFAGRVPSPTHSGTNPYREVRRIVTG
                     PLPHAVASGDLDGDGAEELVVAARRHDDVSIFRREGDAWERVGQLFLDGYPGDLRLAD
                     TDGDGALDLLATDQRFNWLYQWRNRGDGTFEEPVRLITGVYPAALVIADFNGDGKPDA
                     ATAEYDVRRGSATIFLNRGDGRLVHRLSLDMEKNAIALAAGDVDQDGRADLVVACSGN
                     DRILVLYGSGDGTFSRRITLISAHEPYAVALLDANRDGWPDIAAACEGEDVVSYFAGK
                     NEHRFEARVDLPAGHEPYALAVADWNEDGRDDLVVANKGSHSLTLFESSLDGQPERID
                     DVPGGEEPSRLLLADIDGDRRTDLVSVCEGEGALRFFRNESAGTSVREFTAGFENGRV
                     RLAWQVAFDRQAGTAYEIFRRFGDGPWQPVGSRVAASSRVEVVDAWPAEANPEYRIDE
                     FEANFPVRQIGPIRITAPVPEAPGIRLDAANPGSGPVRLVVETPVTGPARLDVFDIQG
                     RRVAGLLDDVVPAGRRVIDWAGTNGGGAPLASGIYLVRLEAAGAERVVRLVRAR"
BASE COUNT          453 a         1021 c         1064 g          521 t
ORIGIN      
        1 acgtctacgt ggccgaaccg gtgaacggac gtttcctcca gttcacgact gccggaagcg
       61 tggtggcaac ctggcaaggc gtcggtcggg acgtggcgcg cggcgtggga gatgatctct
      121 tcgtcatcga caactatgtc acccggatcc gtcgctttac ggcccgggaa atcgactgcg
      181 gcccgccggc accgttaccg aacggcgcgc cgggcatcgc gatcggcgtg cacgtcaccg
      241 aggcgggttc gcccggaccc tgcctgtcgg gcgtcacctt cgactgcacg caatcggtga
      301 cacgcggggg cctggcctcg ccggacatcg gaccctggta ttacgtgtat gtcctggcca
      361 aggtcgacac gatgctcgaa gtggggggcc ttcagtgcgg cgtcatgtac cagggaggaa
      421 gtccgtcggg cctccaggac caggtggggc tcgatgtcta cagctggacg agttgcgccg
      481 actttcaatt tgccgatccg gcctggccgg ccccgggcac gggaaatctc atcgcctggg
      541 atcccgacag ccggtgccag cgcacccaga gtgccatcgc cggctttttc tatgtcggcg
      601 cctacacacc cgatgccctg tcgatcacgg cacaccccgc gaccggcgtg gccacgctgg
      661 tcgactgtca cttccagcac cggcccgtcc acccgcagcg gctcggctgg gccccgttct
      721 cccctggtgt ggacgagtcc ggctgcaatc cgtgccggtc catccggtgt gcgccaccaa
      781 cctccggcgt gccgggcgtc caggcacttt cgtccggccc cccggtgcca aacccgttcc
      841 gctcctcggt ttcgttccga tacacgcttc ccgcgccggc aacggtcgaa gcggcgatct
      901 tcgacatcca gggccggcgc atccgcgcgc tgcctgccgt gcgccagccg gtgggggagc
      961 acgagttccg gtgggacggc cgggacgacg ccggccacga cgccgccagc ggagcctatt
     1021 ttgcccgtgt tgtcgccggg agccgggtcc tggaaaagcg cctgctgcgc atcccctgag
     1081 cggcggccgg ttccccattg ccgtcccgaa gaaccgggac taatctcaat tgacccctcg
     1141 agtccgcgac cgttctccga ggaggtagtc atggtccccg ccgcgtttgc cctcctggcc
     1201 gtcctggccc ccgttcccga cgtttccttt gccggccggg tgccgtcccc cacccattcc
     1261 ggtacgaatc cctaccgcga ggtccgtcgc atcgtgaccg gccccctgcc gcatgcggtt
     1321 gcctccggcg acctggacgg cgacggggcg gaggaactgg tcgtcgccgc ccggcggcat
     1381 gacgacgttt cgatcttccg gcgggaggga gacgcgtggg agcgggtcgg ccagctcttc
     1441 ctggatggct acccgggcga tcttcgcctg gcggacacgg atggagacgg ggcgctcgac
     1501 ctcctcgcca ccgaccagcg gttcaactgg ctgtaccagt ggcggaaccg gggggacggc
     1561 acgttcgagg agccggtccg gctcatcacc ggcgtttatc cggcggcgct ggtgatcgcc
     1621 gatttcaatg gcgacggcaa acccgacgcg gccaccgcgg aatacgatgt gaggcgcggc
     1681 tcggccacca tcttccttaa tcgtggagac gggcggctcg ttcaccggct ttcgctcgac
     1741 atggagaaga acgccatcgc cctggcggcc ggtgatgtcg accaggacgg ccgggccgac
     1801 ctggtggtgg cctgctcggg gaacgaccga atcctggtgt tgtacggctc gggcgacggc
     1861 accttctcgc ggcgcatcac gctgatcagc gcgcacgagc cctacgccgt ggcgctgctg
     1921 gatgccaacc gcgatggctg gcccgacatt gcggccgcct gcgaagggga ggatgtcgtc
     1981 tcgtattttg ccggaaagaa cgagcaccgc ttcgaggcgc gggtggacct gccggcggga
     2041 cacgagccct acgcgctggc cgtggccgac tggaacgagg atgggcgcga cgacctggtg
     2101 gtggcgaaca aggggtcgca ctcgctgact ctcttcgaaa gcagcctgga tgggcaaccg
     2161 gagcggatcg acgacgtgcc cggcggcgag gaaccgagcc gcctcctgct cgcggacatc
     2221 gacggcgatc gccggaccga cctggtgtcg gtgtgcgaag gagagggcgc cttgcgcttc
     2281 ttccgcaacg aatcggccgg gacaagcgtg cgcgagttca cggccggttt cgagaatggc
     2341 cgcgtgcggc tcgcctggca ggtggcattt gatcgacagg cggggaccgc gtacgaaatt
     2401 ttccgtcggt tcggcgatgg cccgtggcag ccggtgggga gtcgtgtcgc ggcatccagt
     2461 cgtgtcgagg tggttgacgc gtggccggcc gaagcgaacc ccgaatatcg gatcgacgag
     2521 ttcgaggcga atttcccggt gcgccagatc gggccgattc gaatcacggc gccggtgccc
     2581 gaagcgcccg gcatccggct cgatgcggcc aaccccggaa gcggcccggt tcggctggtc
     2641 gtggaaacgc cggtgaccgg gccggcccgc ctggatgtgt tcgacattca gggtcgacgt
     2701 gtggcgggac tcctggatga cgtcgttccg gccggccggc gcgtcatcga ctgggctggc
     2761 acgaacggcg gcggtgcccc gctggcgtcc ggcatctacc tggtccggct cgaggcggcc
     2821 ggggcggagc gggtggtgag gttggttcgg gcgcggtgag ggggggcggg cgccactccg
     2881 actgaaggcc aggcctcctg atgtcgaaat accaggggaa tgaccatatc gcccttcgac
     2941 atcgtcgacc tcagcgccgg ggggagccct gagttcctcg tggcttccga tcctcgaacc
     3001 gtgctatcct gttgatcgac aatgatatcc gtcatcatcg tcaattaccg gaccccgga
//
LOCUS       DASVOD010000631         3303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_44300, whole genome shotgun sequence.
ACCESSION   DASVOD010000631 DASVOD010000000
VERSION     DASVOD010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44300"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VF720_13695"
     CDS             complement(<1..559)
                     /locus_tag="VF720_13695"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX7880462.1"
                     /translation="MSDPKPTGGLKLGRLILTAVLTIGLVALLLTQVSIEKIANMIRG
                     ADPGLLMLATAAYALSYVGRTWRWKLLTPGHELGWGPLIGISTVHNFMLRALPSKIGE
                     ASYLVLMRGRGVPGTEALAALVVARIYDTIAAIIFFGVSVLLTHTALAGSRTAAGIVI
                     LLILAGAAFAVLKGSLMVKWMRMLAH"
     gene            complement(556..1851)
                     /locus_tag="VF720_13700"
     CDS             complement(556..1851)
                     /locus_tag="VF720_13700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7880463.1"
                     /translation="MTNSRPAASPADKRLAWALFLGALVIRLAYIALRDLGQAVPAGT
                     DAVSYDAFARAILAGTGWIAHPGPELFRPPGFPMILAVQYAIFGGNLDLVQLSQAVVG
                     AASVVLVFEFGRRQGHRSAALLAALWLMVNPLHLDFAGKLLRENWLVLLNVAVIASLL
                     ADAGLKPGGIIRTGLLFTFLMHIDSRYVFHLPFFALYLLLAQPDTACPFSTRRLSSAV
                     RPTILFVIVVLAASAPWAIRNAVAYDRFVLIDPRTLDRWVGGRHDAMNQAGDPAKESL
                     PAFEAAKSARLDSLSTEERAAFRAGVRPRSGAVNKAIFNFTEFWRVWHTNDEYRPFPD
                     TRFAGRWSTEHNASSLLFMGALLPFFLVGVVRGVAALDRPVLVMTAFIAVHTLLHVAV
                     HSVTRYRLPVEPLYALIAFNALVALMPKKEPMPLPERTA"
     gene            2025..>3303
                     /gene="asnB"
                     /locus_tag="VF720_13705"
     CDS             2025..>3303
                     /gene="asnB"
                     /locus_tag="VF720_13705"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX7880464.1"
                     /translation="MCGILGWWNDRPIDRSLFESALDRMSHRGPDDRGSWYHRTDADG
                     GELALGHRRLSILDLSPRGHQPMTSHDGQRTITYNGEVFNFMDVRRELEAKGHTFASD
                     SDTEVMLEAWLVWGPESLHRLNGMFAFGMWDESAKALHLVRDRLGVKPLYWTEGPWGI
                     AFASEIKSLLALPNVPRRLNERRLAHYLNFLWVPGPETMFEGIHKLEPGHRLVRKNGR
                     NEIHRWWDVPPTEDHARTEGEWIEQLRSLMDDSVRLRLVSDVPVGAFLSGGVDSSAIL
                     AAMRRHTKGELLAYAVGFRGQDQRYEFAPDDLRYARMVADQTKELDYHEIVVQPNVAE
                     LLPKIVWHMDEPVADAAAISTYLICRASREKATVMLSGVGAEEVFGGYRRHHAVQLAE
                     DYLKWPGLVRKGVQALSDALPGSAPGPGLAMRRN"
BASE COUNT          582 a         1055 c         1174 g          492 t
ORIGIN      
        1 gatgggccag catccgcatc cacttgacca tcaggcttcc cttgagcacg gcgaaagcgg
       61 cgccggccag gatgagcagg atgacgatgc cggctgccgt gcgcgagccg gccagcgccg
      121 tgtgggtgag cagcaccgac acgccgaaga agatgatggc ggcgatcgtg tcgtagatgc
      181 gggccaccac cagcgcggcc agcgcctcgg tgcccggcac gccgcggccg cgcatcagga
      241 cgaggtagct cgcctcgccg atcttggagg gaagcgcgcg cagcatgaag ttgtggaccg
      301 tcgagatgcc gatcagcggc ccccagccga gctcgtgccc cggggtcagc agcttccatc
      361 gccacgtgcg cccgacgtac gacagggcgt aggccgccgt tgccagcatc aggagcccgg
      421 ggtcggctcc ccggatcatg ttggcgatct tctcgattga cacctgcgtg aggaggagcg
      481 ccacgaggcc gatcgtcagg acggccgtca ggatgaggcg gccgagcttc aagccgccgg
      541 tgggtttcgg gtcgctcatg cggtccgttc cggtagcggc atcggctctt tcttcggcat
      601 gagcgccacg agggcgttga acgcgatgag ggcgtaaagg ggttccacgg ggagacggta
      661 ccgcgtcacg ctgtggaccg ccacgtgcag cagcgtgtgg acggcgatga aggccgtcat
      721 gacgaggacg ggacggtcga gcgcggccac tcctcgaacg actccgacca ggaagaacgg
      781 cagcagcgcc cccatgaaga ggaggctcga cgcgttgtgc tcggtgctcc atcgcccggc
      841 aaaccgcgtg tcggggaagg ggcgatattc atcgttcgtg tgccatacgc gccagaactc
      901 ggtgaagttg aagatcgcct tgttgacggc gcccgacctg gggcgcacgc cggcccggaa
      961 ggcggcgcgt tcctccgtgg agagcgagtc gaggcgcgcg gacttggcgg cttcgaacgc
     1021 cggcagcgat tccttggccg gatcgcccgc ttggttcatg gcgtcgtgcc ggccgcccac
     1081 ccagcggtcg agcgtgcgcg ggtcgatcag gacgaatcgg tcgtacgcga cggcattgcg
     1141 gatggcccag ggagcactcg cggcgaggac cacgatgacg aagagaatgg tcggcctgac
     1201 ggcggatgac agacgacgcg tcgagaaggg gcacgcggtg tccggctggg ccagcaggag
     1261 gtatagcgca aagaacggca ggtgaaacac gtagcgcgag tcgatgtgca tgaggaacgt
     1321 gaagaggagg ccggtgcgga tgatgcctcc gggtttcagt ccggcgtccg ccagcagcga
     1381 cgcgatgacc gccacgttca ggagcaccag ccagttttcg cgcagcagct tgccggcaaa
     1441 gtcgaggtga agcgggttca ccatcaacca gagggcggcg aggagggcgg cggagcgatg
     1501 gccctggcgg cgcccgaatt cgaagaccag caccaccgag gccgcgccga cgacggcctg
     1561 gctcagctgc acgaggtcga gattcccgcc gaagatggcg tactggacgg ccaggatcat
     1621 cgggaatccc ggggggcgga agagctccgg gcccgggtgg gcgatccagc cggtgccggc
     1681 caggatggcg cgggcaaagg cgtcgtacga gacggcatcg gtgccggccg gcaccgcctg
     1741 tcccaggtcc ctgagcgcga tataggccag tcggatcacc agtgcgccca gaaacagggc
     1801 ccaggcgagg cgtttatcgg cgggggaggc ggcggggcgg gaattggtca cggtttgaga
     1861 tcggccgggt gcggccgata atgaacgggg cgctcgttcc ggttgacgag ggccgggcgg
     1921 ccacgtagac tttcccgttc cataagtcgc aacagtttac cgacttatgc ccctgaatca
     1981 aagcgccatc tcaaccgggc gccgatggag acctgaatcc ccccatgtgc ggaatcctgg
     2041 gctggtggaa cgaccgcccg attgatcgct ccctcttcga gtccgcgctc gatcggatga
     2101 gccaccgggg cccggacgac cgcggctcct ggtatcaccg gaccgacgcg gacgggggcg
     2161 agctggccct cgggcaccgg cgcctctcca tcctcgacct ctcgccgcgc ggccaccagc
     2221 cgatgaccag ccatgacggc cagcgcacga tcacctacaa cggcgaagtc ttcaatttca
     2281 tggacgtccg ccgcgagctc gaggcgaaag ggcacacctt cgccagcgac tccgacaccg
     2341 aggtgatgct cgaggcctgg ctggtctggg gccccgaatc gcttcaccgg ctcaacggaa
     2401 tgttcgcctt cggcatgtgg gacgaatccg cgaaggcgct ccacctcgtg cgcgaccgcc
     2461 tcggcgtgaa gccactctac tggaccgagg ggccatgggg gatcgcgttc gcgtcggaga
     2521 tcaagtcact cctcgcgctc cccaacgtgc cgcggcggct caacgagcgt cgtctggcgc
     2581 actacctcaa cttcctctgg gtgcccgggc cggagacgat gttcgagggg atccacaagc
     2641 tggaacccgg gcatcgcctc gtgcgaaaga acggccgcaa cgagatccac cgctggtggg
     2701 atgtgccgcc gaccgaggat cacgcccgca ccgaagggga gtggatcgag cagctccgct
     2761 ccctcatgga cgacagcgtg cgcctgcgcc tcgtttccga tgtgccggtc ggcgcgttcc
     2821 tgtcgggcgg cgtcgattcg tcggccatcc tggcggcgat gcgacgccac acgaagggcg
     2881 agctcctggc ctacgcggtc ggctttcgcg gccaggacca gcggtacgaa tttgcgccgg
     2941 atgacctgcg ctacgcgcgg atggtggccg accagaccaa ggaactcgat taccacgaga
     3001 tcgtcgtgca accgaacgtg gccgaactcc tgccgaagat cgtatggcac atggacgaac
     3061 cagtcgccga cgcggccgcc atctcgacgt acctgatctg tcgcgcctcg cgcgagaagg
     3121 ccacggtgat gctctccggt gtgggcgccg aggaagtgtt cggcggctat cgccggcacc
     3181 acgcggtgca gctcgccgag gactacttga agtggccggg cctcgtgcgg aaaggcgtgc
     3241 aggccttgtc ggatgcgctt cccggctcgg cgccgggacc ggggcttgcc atgcgacgca
     3301 atc
//
LOCUS       DASVOD010000632         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_147551, whole genome shotgun sequence.
ACCESSION   DASVOD010000632 DASVOD010000000
VERSION     DASVOD010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147551"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VF720_13710"
     CDS             <1..487
                     /locus_tag="VF720_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX7880465.1"
                     /translation="LSREALYEIHLAGLLHDIGKIAVPDEVLMKPGRLTDLEMRQVQA
                     HVEWSWNILSPITMLGPVGLMVRHHHERFDGHGYPDRLAGDAIPQGARIMAVADMFVA
                     MTEDRPYRKGLPVEVAVAELQRVSGSQVDPALVRSFLECLEIDGLYEPIVSESKRAAA
                     N"
     gene            complement(442..1449)
                     /gene="ftsY"
                     /locus_tag="VF720_13715"
     CDS             complement(442..1449)
                     /gene="ftsY"
                     /locus_tag="VF720_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX7880466.1"
                     /translation="MFDLFKKLKESLTRTRDTLTEGVRTVLAGFRPIDESLFEELEEV
                     MIGADVGVATTEKLLDRLRQRVKSAGVRDSSGVEAILREEIAAMLSAGGAAGATPGTP
                     STTMSNGVGSSAAGDVPRLPDGSPAPRVVIVIGVNGVGKTTTIGKLAWREAGQKKKVV
                     IAASDTFRAAASEQLGIWAERAGVDIVRSSHGADPAAVAFDGLSAAISRRADLLIVDT
                     AGRLQTKHHLMDELKKIGRVLSGQLPGAPHEVLLVLDASTGQNAVIQAREFGKTLGVT
                     GLVLTKLDGTAKGGVVLAIADQLGLPVRWVGVGESIEDLAEFDPAQFAAALFDSETIG
                     S"
     gene            complement(1468..>3074)
                     /locus_tag="VF720_13720"
     CDS             complement(1468..>3074)
                     /locus_tag="VF720_13720"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7880467.1"
                     /translation="LGERIVAEGELDELRRTIAALLTQRAARQAEVGRLEGERNGVDE
                     READLVLREASHTARRTALADNRHRLEGALHAASDDSKTAETAYQEEEAALMRLDADR
                     ESARSVLNETRIAAATREAALSECRERIERVKATAREIEVDNERCVVEVSEGEERLVT
                     LAADVEVLASEVQQLHGGRDGRVEAVTEKERAVADLTISVTAADGAAREARKAASELR
                     EQAHGEELLRTELKAEFRNLEVRLYEDYDQTTQELLAAPRDWPVTEAGEDAVLEVVET
                     DVMEYKEKIKRLGPVNLLAIEEYEEKATRLTFLKGQFDDLIQARDSLNEAIAQINTTA
                     SELFLSTFRQVQENFQKTFLVLFQGGECSLTLVGDDPLECEIEIVAKPRGKRPQSIAQ
                     LSSGERALTAIALLFAIYLVKPSPFCILDEVDAPLDDANVDRFVAMIREFSSRTQFIV
                     ITHNKKTMEACDTLYGVTMQTAGISQVVSVKLNEADAVLEGKKGTAAKPGRIDAREAL
                     PAMAFPDGSALEASEAGEPAVDPVAQ"
BASE COUNT          427 a         1056 c         1049 g          542 t
ORIGIN      
        1 tctctcgcgc gaggccctgt atgaaatcca cctggccggg ctgctgcacg acatcggcaa
       61 gatcgccgtg ccggacgagg tgctcatgaa gcccggccgc ctgaccgacc tcgagatgcg
      121 gcaggtgcag gcccacgtgg agtggtcgtg gaacatcctg tcgccgatca cgatgctggg
      181 gccggtggga ctcatggtcc ggcatcacca cgagcgcttc gacggacacg gctatcccga
      241 ccgcctggcc ggcgacgcaa ttccgcaggg tgcgcggatc atggccgtgg ccgacatgtt
      301 cgtggcgatg accgaagacc gcccttaccg caagggtttg ccggtggagg tggccgtggc
      361 cgaattgcag cgcgtgtcgg gaagccaggt cgatccggcg ctggtgcgct cgttcctcga
      421 gtgtctcgag atcgacggcc tctatgagcc gatcgtttcg gagtcgaaga gagccgccgc
      481 gaactgagcc gggtcgaatt cggccaggtc ctcgatcgac tcaccgactc ccacccagcg
      541 caccggtaac ccgagctggt cggcgatggc cagcaccacg ccccccttgg ccgtgccgtc
      601 gagtttggtg aggacgaggc ccgtcacgcc cagcgtcttc ccgaattcgc gcgcctggat
      661 gaccgcgttc tgccccgtcg aggcatcgag cacgagcagc acctcgtggg gcgctcccgg
      721 cagctgcccc gagaggacgc ggccgatctt cttcagctcg tccatcagat gatgcttggt
      781 ctgcaggcgc cccgccgtgt cgacgatcag gaggtcggcc cgccgggaga tggccgcgga
      841 taatccgtcg aaggccacgg ccgccgggtc ggccccgtgg ctcgagcgca cgatgtcgac
      901 gccggcccgt tcggcccaga tgccgagctg ctcggaggcc gccgcgcgga aggtgtccga
      961 ggcggcgatg acgacttttt tcttctggcc tgcctcgcgc caggcgagct tgccgatggt
     1021 ggtggtcttg ccgacgccat tgacgccgat gacgatgacg acgcggggcg ccggcgagcc
     1081 gtcgggaaga cgaggcacgt caccggcggc ggatgagccg actccgttcg acatggtggt
     1141 ggatggcgtt cccggtgtgg ctccagccgc tccccccgcc gaaagcatgg ccgcgatctc
     1201 ctcgcggagg atggcctcca cgcccgagga gtcgcgcacg ccggccgatt tcacgcgctg
     1261 ccggaggcga tcgaggagct tctcggtggt ggccacgccg acgtccgcgc cgatcatcac
     1321 ttcctcgagt tcctcgaaga gcgattcatc gatgggccgg aagccggcca gcaccgtgcg
     1381 gactccctcg gtcagggtgt cgcgcgtcct ggtgaggctc tccttgagct tcttgaagag
     1441 gtcgaacatg gaacgcccgg cccgggacta ctgcgccacg gggtcgacgg ccggctcgcc
     1501 ggcttcggat gcctcgaggg ccgaaccatc gggaaaggcc atggccggca gcgcctcgcg
     1561 ggcgtcgatc cgtccgggct tcgcggcggt ccccttcttc ccttccagca ccgcgtccgc
     1621 ctcgttcagc ttcaccgaca cgacctggct gatgccggcc gtctgcatcg tgacgccgta
     1681 cagcgtgtcg caggcctcca tcgtcttctt gttgtgcgtg atgacgatga actgcgtgcg
     1741 tgaggagaac tcgcggatca tggcaacgaa ccggtcgacg ttggcgtcgt ccagcggggc
     1801 atccacttcg tccaggatgc agaaggggct cggcttcacc aggtagatgg cgaagaggag
     1861 cgcgatggcc gtgagggccc gctcgccgct cgagagctgg gcgatcgact gcggcctctt
     1921 cccgcgcggc ttggccacga tctcgatctc gcattccagc ggatcatccc ccaccagcgt
     1981 gagggagcac tcgccaccct ggaagaggac gaggaacgtc ttctggaagt tctcctgcac
     2041 ctgccggaac gtggagagga agagttcgga cgccgtcgtg ttgatctggg cgatggcctc
     2101 gttcagcgag tcccgggcct ggatcaggtc gtcgaactgc cccttgagga aggtgaggcg
     2161 cgtggccttc tcctcgtact cttcgatggc caggaggttg acgggcccca ggcgcttgat
     2221 cttctccttg tactccatga cgtcggtttc cacgacctcg agcacggcat cctcgccggc
     2281 ctcggtcacc ggccagtcgc gcggcgcggc caggagctcc tgggtggtct ggtcgtagtc
     2341 ttcgtagagc cgcacttcca ggttgcggaa ctcggccttg agctcggtgc gcaggagttc
     2401 ctcgccgtgg gcctgttcgc gcagctcgga ggcggccttg cgtgcctcgc gggccgcgcc
     2461 gtcggccgcg gtgacgctga tcgtgaggtc ggccaccgcg cgttccttct cggtgacggc
     2521 ctccacgcgt ccatcgcgcc cgccgtgcag ctgctgtacc tcggaggcca ggacctcgac
     2581 atcggccgcg agcgtgacga ggcgttcctc cccttcggac acctccacca cgcatcgctc
     2641 gttgtcgacc tcgatctcgc gggcggtggc cttcacccgc tcgatccgct cccggcactc
     2701 cgagagggcc gcctcgcgcg tggcggcggc gatgcgggtt tcgttcaaca ccgagcgggc
     2761 cgactcgcgg tcggcgtcga ggcgcatcag ggccgcctct tcctcctggt aggccgtttc
     2821 ggcggtcttg ctgtcgtccg aggccgcatg gagcgccccc tccagccgat gccggttgtc
     2881 ggccagcgcc gtacgccggg ccgtgtggga ggcttcgcgc agcacgaggt ccgcctcgcg
     2941 ctcgtcgacg ccgttccgtt ccccttccag gcgcccgacc tcggcctggc gcgccgcccg
     3001 ctgggtcaag agggcggcga tggtgcgacg cagttcgtcg agctcccctt cggccacgat
     3061 ccgctcgccc aagg
//
LOCUS       DASVOD010000633         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1036616, whole genome shotgun sequence.
ACCESSION   DASVOD010000633 DASVOD010000000
VERSION     DASVOD010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036616"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1620
                     /locus_tag="VF720_13725"
     CDS             <1..>1620
                     /locus_tag="VF720_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX7880468.1"
                     /translation="DNLNPVQREAVTHTEGPVLILAGAGSGKTRVLTHRIAWLVRRLG
                     VHPSEVLAFTFTNKAAGEMKERVAHLLGTPIGSMWIGTFHSTCVRLLRASGSHRGLDR
                     NFVIYDSDDQETLVRRLLADQSLGDKEFRPRAVLSKISNIKNRLITPEAYRGMTTTYH
                     EELIAKIYAEYQKALKKAHALDFDDLIGESVALLAENEAVREQYARRFKYVHVDEYQD
                     TNGPQYELIRHLASVHRNLCVVGDDDQSIYGWRGADIANILSFEKTYPDAFVVRLEQN
                     YRSTGNILAAANAVVRNNRKRKEKTLWTEETSGELLKVTIVGDEEAEGELIVSGMTRA
                     VHDDGRSLRQLAVLYRTNAQSRAIENALRRAAVPYELVGGTPFYSRREVKDLLAYLRL
                     SINPNDDVAFRRVLNVPKRGIGKTTLDKLAAAAFRDGVPMLGAARRLREDGFFGAASL
                     DITAGTREKIAAFVAIVDGLAARGNEPVSELLSSLVTTLDFGRHLMDDDPETAADRIE
                     NVEELIVGARQYVERATDAGVAAFLNEVALLT"
BASE COUNT          294 a          567 c          519 g          240 t
ORIGIN      
        1 gacaacctga atcccgtcca gcgggaggcg gtcacccaca ccgagggacc ggtcctcatc
       61 ctggccggcg ccggttccgg caagacgcgc gtcctcacgc accggatcgc ctggctggtg
      121 cggcgcctgg gcgtgcaccc ctccgaagtc ctcgccttca ccttcaccaa caaggcggcg
      181 ggggagatga aggagcgggt ggcccacctg ctcggcaccc cgatcggctc gatgtggatc
      241 ggcacgttcc actccacctg cgtgcgcctc ctgcgggcgt cgggaagcca ccgcggcctc
      301 gaccggaact tcgtcatcta cgactccgac gaccaggaaa cgctggtgcg ccgcctgttg
      361 gccgaccagt cgctggggga caaggaattc cgcccgcggg cggtcctctc gaagatctcc
      421 aacatcaaga accggctcat cacgcccgag gcctaccggg ggatgacgac cacgtaccac
      481 gaggaattga tcgccaagat ctacgccgaa taccagaagg cgctgaagaa ggcccatgcg
      541 ctcgacttcg acgacctgat cggcgagtcg gtggcgctcc tggccgagaa cgaggcggtg
      601 cgcgagcagt acgcgcgccg tttcaagtac gtgcacgtgg acgagtacca ggacaccaac
      661 gggccgcagt acgagctcat ccgccacctg gccagtgtcc accgcaacct gtgcgtagtg
      721 ggagatgacg accagtcgat ctacgggtgg cgcggcgccg acatcgccaa cattttgtca
      781 ttcgagaaga cctaccccga tgccttcgtg gtgcgcctcg agcagaacta tcgctccacc
      841 ggcaacatcc tggccgccgc caatgcggtg gtccggaaca accgcaagcg gaaggaaaag
      901 actctctgga cggaggaaac ctccggcgag ctcctcaagg tcacgatcgt gggggatgag
      961 gaggccgagg gggagctcat cgtgagcggc atgacgcggg ccgttcacga cgacggccgc
     1021 tccctcaggc agctggcggt gctctaccgc accaacgccc agtcgcgcgc catcgagaac
     1081 gcgctgcgcc gggcggcggt gccgtacgag ctggtgggag gcacgccctt ctactcccgg
     1141 cgcgaggtaa aggacctcct ggcctatctc cgcctctcca tcaatcccaa tgacgacgtg
     1201 gccttccgcc gcgtcctcaa cgtccccaag cgggggatcg gcaagaccac gctcgacaag
     1261 ctggccgccg ccgccttccg tgacggcgtg ccgatgctcg gggcggcccg gcgcctccgc
     1321 gaggatggct tcttcggcgc cgccagcctc gacatcaccg ccggaacgcg cgagaagatc
     1381 gccgccttcg tggccatcgt cgatggcctc gcggcacgcg gcaacgagcc ggtgtcggag
     1441 ctcctctcct cgctggtcac cactctcgat ttcggccggc atctcatgga tgacgacccg
     1501 gaaaccgccg ccgaccggat cgaaaacgtg gaggaactga tcgtgggcgc ccggcaatac
     1561 gtggagcggg ccaccgacgc gggcgtggcc gccttcctga acgaggtggc cctcttgacc
//
LOCUS       DASVOD010000634         2938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_770894, whole genome shotgun sequence.
ACCESSION   DASVOD010000634 DASVOD010000000
VERSION     DASVOD010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770894"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="VF720_13730"
     CDS             <1..227
                     /locus_tag="VF720_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880469.1"
                     /translation="KVITGYTPELQKIFRAMKTYGLILADNGTDMYIQGTMDPGWDND
                     ILNPAFHGLYADDFEVIQLGWQPMTTGVPE"
     gene            complement(251..2782)
                     /locus_tag="VF720_13735"
     CDS             complement(251..2782)
                     /locus_tag="VF720_13735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX7880470.1"
                     /translation="MNRCRPGSPLVRGLLLALLALSLVASGCGPSRTVDVSRLRAYQP
                     GGATVLYDKDGRPFADLAPVDRVLVGLDDLPPHVPAAFIAVEDKRFYQHNGVDTWRVG
                     GAIFHNLKQGGLEEGFSTITMQLARSVFADQIPLSERNVQRKWREMQAAMAIEKRFSK
                     KEILELYLNHVYFGKGAVGIQGASRHFFGKDAGDLTLGEAAMLAGLLKAPARFDPYSH
                     PDKARERRDLVLRMMQDQKRVTPNEVAEARASGLGVRPGRAGRGVPGDVGAWFVEEVR
                     RQLEKDFGDRLYTQQLKVYTTIDVDLQSAAEEELARQLNSIERGQYGRYTGARYNAST
                     QPTGTATPYLQGAIVAMDVSTGDVRAWVGGRDIRQSEFDRVSSAHRQAGSSFKPFVYA
                     AAIADGVPLTRRLEDQPYTVQLARTQTWEPKNFSGDYSGHVTMRNALVRSVNVPTVHL
                     AEEVGYRRITTLAKGAGIVSPIAETPAMALGTVAVSPLELTSAYTTFAGLGRHVAPRL
                     ITRIENEAGEVLRTYAIDARDVLPPSVAYLINDTLHDAVQRGSGAPAMQAGFTGPAAG
                     KTGTTSDGRDAWFVGYTPEIVAGVWIGFDTPKRIIAGASGGRLAAPAWGRMMSRYYGN
                     SEPSWWPAPANVVEYDIDPETGRLLEEGCRPRWGETSTEIFIRGTEPESICPESDYEY
                     TWFNVSGSGDGQNGGDGYESDEEARQGRPDVDEERHDEATPEIDLDGGWIFELGINDT
                     NDRNLRNYTATWQVRLWVDGNDVHGSGEKIAEDGRRVDSDRRTSISLSGQVNGESLRL
                     RLTEYNGEGALSGWFNVDLEGRRLRGEFVSSDAATRGAVSARRSD"
BASE COUNT          461 a          979 c         1001 g          497 t
ORIGIN      
        1 gcaaggtgat cacgggttac acgcccgagc tgcagaagat ctttcgggcc atgaagacgt
       61 acggcctcat cctggccgac aacggcaccg acatgtacat ccaggggacg atggacccgg
      121 gctgggacaa cgacatcctg aaccccgcgt ttcacggcct gtacgccgac gacttcgagg
      181 tgatccagct gggttggcag ccgatgacga cgggcgttcc ggagtagcgg gagcgacggc
      241 ggcgcgcagg ctagtcgctg cgccgcgcgc ttaccgcacc gcgcgtggcg gcgtctgagc
      301 tcacgaattc gccgcgcaga cggcgaccct cgagatcaac gttgaaccag ccgctcagcg
      361 cgccctcgcc gttgtattcg gtgaggcgca ggcggaggct ctcgccgttc acctgacccg
      421 acaacgagat ggacgtgcgg cgatcgctgt ccacccggcg gccgtcctcg gcgatcttct
      481 cgccgctgcc gtggacgtcg tttccgtcca cccagagacg cacctgccag gtggcggtgt
      541 agttgcgcag gttgcggtcg ttcgtgtcgt tgatgcccag ctcgaagatc cagcctccgt
      601 cgaggtcgat ttccggcgtg gcctcgtcgt ggcgctcctc gtcgacatcg gggcgcccct
      661 gccgagcctc ctcgtcgctt tcgtagccat cgccgccatt ctgcccgtcg ccgctgccgc
      721 tcacgttgaa ccaggtgtat tcgtaatccg attcggggca gatcgactcg ggctcggtgc
      781 cacggatgaa gatctcggtg gaggtctcgc cccagcgggg acggcaccct tcctcgagga
      841 ggcggccagt ctccggatcg atgtcgtact ccaccacgtt ggcgggcgcc ggccaccacg
      901 agggttccga gttgccgtaa tagcgactca tcatgcgccc ccatgccggc gcggccaggc
      961 ggccacccga ggcgccggcg atgatccgct tcggtgtgtc gaaaccgatc cagacgccgg
     1021 caacgatttc gggcgtgtaa ccgacgaacc aggcgtcccg gccgtcggag gtggtgccgg
     1081 tcttgccggc ggccggcccg gtgaagccgg cctgcatggc cggcgcgccg ctgccgcgct
     1141 gcaccgcgtc gtgcagcgtg tcgttgatga gatatgccac cgacggcggc agcacgtcac
     1201 gcgcgtcgat ggcataggtg cgcagcactt cccccgcctc gttctcgatg cgcgtgatca
     1261 gccggggggc cacatggcgc ccaaggccgg cgaacgtggt gtaggccgag gtgagttcga
     1321 gcggcgacac cgccacggtg cccagcgcca tggccggcgt ttcggcgatg gggctcacga
     1381 tgccggcccc cttcgccagc gtggtgatgc gccggtaacc cacctcctcg gccaggtgca
     1441 ccgtgggcac attgaccgag cggacgagcg cattccgcat cgtgacgtgc cccgaatagt
     1501 cgccgctgaa gttcttcggt tcccaggtct gggtgcgggc gagctgcacg gtgtagggct
     1561 ggtcttcgag acggcgtgtg agcggaacgc cgtcggcgat ggccgccgcg tagacgaacg
     1621 gcttgaacga gctccccgcc tgccgatgcg ccgacgacac gcgatcgaat tccgactggc
     1681 ggatgtcgcg cccacccacc cacgcgcgca cgtcgcccgt ggacacatcc atcgccacga
     1741 tggcgccctg caggtagggg gtggcggtac cggtcggctg ggtggaggcg ttgtaacggg
     1801 cgccggtgta gcggccgtac tgcccccgct cgatggaatt cagctggcgc gccagctcct
     1861 cctcggccgc cgactgaaga tccacgtcga tcgtcgtgta gaccttgagc tgctgcgtgt
     1921 aaagccggtc gccgaaatcc ttctcgagct ggcggcggac ttcctcgacg aaccaggcac
     1981 ccacgtctcc cggcacaccg cggccggcgc gtccaggccg cacaccgagg ccggaggcgc
     2041 gcgcctcggc cacctcgttg ggcgtcacgc gcttctggtc ctgcatcatc ctgagcacga
     2101 ggtcacgccg ctcgcgcgcc ttgtcgggat gactgtacgg atcgaacctc gccggcgcct
     2161 tgaggagccc ggccagcatg gcggcttcac ccagggtcag atccccggcg tccttgccga
     2221 agaagtggcg cgaagccccc tggataccca ccgcaccctt cccgaaatag acatggttca
     2281 ggtagagctc gaggatttcc ttcttcgaga agcgcttctc gatggccatg gccgcctgca
     2341 tctcgcgcca cttgcgctgc acgttccgtt cgctgagcgg gatctggtcg gcaaagacgc
     2401 tgcgggccag ctgcatggtg atcgtcgaga atccctcttc gaggccaccc tgtttcaggt
     2461 tatgaaagat cgcgccgccg acgcgccagg tatccacgcc gttgtgctgg tagaagcgct
     2521 tgtcctccac ggcgatgaag gcggccggga catggggcgg caggtcatcg agcccgacca
     2581 gcacccggtc gacgggagcg aggtcggcga aggggcggcc atccttgtcg tagagaacgg
     2641 tggcgccgcc gggctgatag gcgcggagtc gactgacatc aacggtgcga ctggggccgc
     2701 agccggacgc cacgagcgaa agggccagga gcgcgagcag aagccctcgc accaggggtg
     2761 agccggggcg gcagcggttc acgggatcgg ggaatcgata ttgaggggcc atgaagacag
     2821 gtgtttgcaa tatccgggcc ggtatttttt accggattgt gcgccggcgg ggcatcacgc
     2881 cgatcggcgt gccggacgac gggtgtaaga cgttctcatc cgccgcttcg ttgcgtcc
//
LOCUS       DASVOD010000635         4478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_771057, whole genome shotgun sequence.
ACCESSION   DASVOD010000635 DASVOD010000000
VERSION     DASVOD010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771057"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /locus_tag="VF720_13740"
     CDS             complement(<1..1492)
                     /locus_tag="VF720_13740"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX7880471.1"
                     /translation="MKPSVNRKPTSPSRMARWAHAAAILMVVSLTCLATPARADDPAP
                     RPPMNAAEIDVALKRLSVVGTALYVAAHPDDENTAVLTWLENVAGVRAAYLSMTRGDG
                     GQNLIGNEKGDLLGLVRTQELLAARRIDGAEQFFTRALDFGFSKNPDETLAFWGRDSV
                     LADVVRTIRYVKPDIIINRFPTTGEGGHGHHTASAILSLAAFEAAGDPGRYPEQVARY
                     GTWKPKRAFLNWFTGARAPDAEVAKTLVTVDVGDYAPLLGKSSNELAADSRSMHKSQG
                     FGSAERRGHQVNYFKVVAGDSLTKGLFDGIDLTWKRVPGGEKLGTEFEAIRTAYDPRN
                     PSASLPALLRADAALATLRQGKGAAAWSDLLERKAADLHEVIRSCAGLWIEAIATDAS
                     FAGGDSLKVNFMAVNRSPVPMTVTRAQLLVGPERAVLATVAVDKAMDDNTPVTAVLNG
                     VVPSSADWTWTQPWWLREPHGKGALAVPDTRLFDVNDTPPLAVAGMD"
     gene            complement(1582..4302)
                     /locus_tag="VF720_13745"
     CDS             complement(1582..4302)
                     /locus_tag="VF720_13745"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7880472.1"
                     /translation="MRPAGRKLLPIVLIGLLAATATAGPPFGTSQRVLRWPAGIQEFV
                     FLAPTGDSRLLSILDRNRGVTFLDLSGPRPLEPLLVIDADASTLDESSDGSLQFEDVT
                     GDGVHDMIVQSEVAFVRAGRPDGTFEEPSSWYMGDDVHELRVGDFDGDGRRDLVVAQG
                     GGEMFYRLGRSDGLFGPLTSRTLDPSASLVGLDDLDGDGDLDLLFATYDYGSDRTIHR
                     FWRNSGAGQFTVLPGDTLSDAGVTWSRFGRHDRDGLPDRFRVVEESGDPEALRFLEVQ
                     RGLGAGRFAPPVRMALVPDNPDYLRDLDGDGLDDFVWNDDRLRVATTQPDGLLGQIED
                     YGASVYSSAFALHDLDEDGRPDLLFAQGGELRIISTRALATTPEPSIVRFHSSRSPLV
                     FDADGDSRPDLVTIEAPFRTGPELAVRPGRSDATFGPPVIAPLDFSIGDDPEPGDFDG
                     DGDTDLHISRRVEAGVEHRLLRNDGLGHFVPGPPVTFEEEESREVVVADFDGDGADDR
                     LQVENDLLSCRIVLLRWSPSGECVRSSGSFDGDLGWLNALDLDGDGDLDLCALTGYGT
                     RIVRFMGAGDGRFERLGTERLSERLYSFHVERVVESGSIQMIGSTYREIFRLEPDGAG
                     RWRLGRRLLDTYARRYLPVPDLNGDGRPELGIFDGIFSGGTLMLLPSSGPDDLGDVIR
                     IGLIGEADRLDFRDMTGDGLSEMIGTSRSGAWVYRGLPVEGGPPSEFRATAIGWNVQL
                     SYRFDPAGDGNVRLVRRAVGSASPDVVLDERPASPHVSLTDTTIPTPGIYDYRLVLDI
                     DDGYPRQVATARVMVAPEGEFRLLPLSPRPGAGPFTLQYASPVPVATMLEIFDVLGRR
                     VRRIDVSLEATPAGTIVWNGRGEDGRPAASGLYFARLADRAGTVRVVLVR"
     gene            complement(4299..>4478)
                     /locus_tag="VF720_13750"
     CDS             complement(4299..>4478)
                     /locus_tag="VF720_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880473.1"
                     /translation="YDVQGRRIWDTTMRPETAGLHCLQWSGCGLTGAPVPAGTYFVRL
                     DSPSGTANTRVTVVR"
BASE COUNT          761 a         1511 c         1525 g          681 t
ORIGIN      
        1 catccatccc ggccacggcc agcggcggcg tgtcgttgac atcgaagagc cgggtgtcgg
       61 ggacggccag cgcccccttc ccgtgcggtt cgcgcagcca ccagggctgc gtccaggtcc
      121 agtcggcaga ggagggaacc acgccattca gcaccgccgt gaccggcgtg ttgtcgtcca
      181 tcgccttgtc gacggccacc gtggccagca cggcccgctc cggtcccacg aggagttgcg
      241 cgcgagtcac cgtcatcggc accgggctcc ggttcaccgc catgaagttc accttgagcg
      301 aatcaccccc cgcgaacgag gcgtcggtgg cgatggcctc gatccagagg ccggcgcacg
      361 aacggatgac ctcatggagg tcggccgcct tgcgctcgag gaggtcgctc caggccgcgg
      421 cacccttccc ctgccgcagc gtggccaggg ccgcgtcggc gcggagaagc gccggcaggc
      481 tggctgaagg attgcgcgga tcatacgccg tacggatggc ctcgaactcc gtgccgagct
      541 tctcgccacc gggaacgcgc ttccaggtca ggtcgatccc gtcgaacagc ccctttgtga
      601 gtgaatcgcc cgccacgacc ttgaagtagt tcacctggtg accgcgccgc tcggccgagc
      661 cgaaaccctg gctcttgtgc atgctgcggc tgtcggcggc cagttcgttg gaggatttgc
      721 cgaggagcgg cgcgtaatcc cccacgtcta ccgtgacgag cgtcttcgcc acctcggcgt
      781 cgggcgcccg cgcccccgtg aaccagttga ggaaggcgcg cttcggcttc caggtgccgt
      841 atcgggccac ctgttcgggg taacgccccg gatcgcccgc ggcttcgaat gccgccagcg
      901 acaggatggc ggaggcggtg tggtgcccgt gccccccctc ccccgtcgtc gggaagcgat
      961 tgatgatgat gtcgggcttc acgtagcgga tggtgcgcac cacgtcggcc aggaccgagt
     1021 cgcgccccca gaacgccagc gtctcgtcgg ggttcttgga gaagccgaag tcgagtgccc
     1081 gcgtgaagaa ctgctccgcc ccgtcgatgc gccgcgccgc caggagttcc tgcgtgcgca
     1141 cgaggccgag gagatccccc ttttcgttgc cgatgaggtt ctggccgccg tcgccccgcg
     1201 tcatcgagag ataggccgcc cgcacgccgg ccacgttctc gagccaggtg agcacggccg
     1261 tgttctcgtc gtccggatgc gccgccacgt agagcgcggt gccgacgacc gacaggcgct
     1321 tcaacgccac gtcgatctcg gcggcgttca tgggtgggcg cggggcagga tcgtccgcgc
     1381 gggcgggggt ggccaggcag gtgagggaaa cgaccatgag aatcgcggcc gcgtgggccc
     1441 atcgcgccat tcgggatggg gacgtcggct tgcggttgac gctcggcttc atgcgggacc
     1501 tttcctccgg tgaaatgcga gcggaaaggg tacgtggtga gacagggagc gccaaggggt
     1561 cggcggggcc gggaccgccg gtcagcggac cagcaccacc cgtaccgtgc cggcccgatc
     1621 ggcgaggcgg gcgaagtaga gacccgaggc ggccggccgg ccatcctctc cgcgaccgtt
     1681 ccagacgatc gtgccggccg gggtggcctc caggctcacg tcgatgcgac gcacgcggcg
     1741 gccaaggacg tcgaagatct cgagcatggt ggcaaccggc accggggagg cgtactggag
     1801 cgtgaacggg cccgcaccag gacgcggcga caatggcagg agccggaact ccccctccgg
     1861 agccaccatg acccgtgcgg tggcgacctg gcgagggtag ccatcgtcga tatcgaggac
     1921 gaggcgatag tcgtagatcc cgggagtcgg gatcgtggtg tcggtcaagg aaacgtgggg
     1981 cgatgcgggg cgttcgtcga gcacgacgtc ggggctggct gaacccaccg cgcgtcgaac
     2041 caggcgtacg tttccatcgc ccgccggatc gaaccggtag gacagctgca cgttccatcc
     2101 gatggcggtc gcgcggaatt cgctcggggg tccgccttcg accggaagtc cccggtacac
     2161 ccacgccccg ctccggctcg tgccgatcat ttccgaaagt ccatccccgg tcatgtcccg
     2221 gaagtcgagc cggtccgctt ccccgatgag tccaatccgg atcacatcgc caaggtcatc
     2281 cggccccgag ctcggcagga gcatgagcgt gccgcccgaa aaaatgccgt caaagattcc
     2341 cagctccggc cggccgtcgc cattcagatc gggaacaggg aggtaacggc gcgcataagt
     2401 gtcgaggagg cgccgtccca gccgccagcg accggcacca tccggctcga ggcggaagat
     2461 ctcccggtag gtcgatccga tcatctggat cgatccactc tcaaccacgc gctccacatg
     2521 aaaggaatag agacgctcgc tgaggcgctc ggtaccgagg cgctcgaacc ttccgtcgcc
     2581 cgctcccatg aatcgaacga ttcgggtgcc gtatccggtc aaggcgcaga ggtcgaggtc
     2641 gccgtcccca tccaggtcga gtgcgttgag ccaccccagg tcgccatcga acgagccgct
     2701 ggagcggacg cattcgccgg acggtgacca tcgaaggaga acgatccggc acgagaggag
     2761 gtcgttctcg acctgcagcc gatcgtcggc cccgtcgcca tcgaaatccg ccacgacgac
     2821 ctcccgactc tcctcctcct cgaaggtgac gggtggtccg ggcacgaagt gaccgaggcc
     2881 gtcgttgcga aggagccggt gctcgacgcc ggcttcgaca cgccggctga tatggaggtc
     2941 cgtgtcgccg tctccgtcga agtctcccgg ctccggatcg tccccgatgg aaaagtcgag
     3001 gggcgcgatc accggcggcc cgaaggtggc gtcggacctt cctggccgca cggccagctc
     3061 gggtcccgta cggaagggtg cctcgatcgt cacgaggtca ggccttgaat cgccgtcggc
     3121 atcgaaaaca aggggtgaac gactcgaatg gaacctcacg atcgacggtt ccggcgtggt
     3181 ggccagtgcc ctcgtcgaga tgatccggag ttcgcccccc tgggcaaaga ggaggtcggg
     3241 gcggccatcc tcgtcgaggt cgtgcagcgc gaatgccgag gagtaaaccg atgcaccgta
     3301 gtcctcgatc tgaccgagca ggccatccgg ttgcgtggtg gccactcgga gccggtcgtc
     3361 attccagacg aaatcatcga ggccgtcgcc gtccaggtcc ctcaggtagt cgggattgtc
     3421 cggcacgagg gccatgcgca ccggcggggc gaatcgtcct gcgccgagcc cgcgctgcac
     3481 ctcgagaaag cgaagagcct cagggtcacc tgattcctcg acgacgcgga accgatccgg
     3541 caggccatcc cgatcgtgtc gcccgaaccg ggaccaggtc acgccggcat cggaaagcgt
     3601 atcgccgggt aagaccgtga actggccggc gcccgagttg cgccagaacc tgtggatcgt
     3661 ccgatcgctc ccgtaatcgt aagtcgcgaa gaggagatcc aggtcaccgt ccccgtccag
     3721 gtcgtccagg cccacgagag acgccgaagg gtcgagtgtc cggctcgtca acgggccgaa
     3781 gagcccgtcg gaccgaccga gccggtagaa catttcgccg ccgccctgcg cgaccacgag
     3841 atcccgccgc ccatccccat cgaaatcccc cacgcgcagt tcgtggacat cgtcccccat
     3901 gtaccagctc gatggctcct cgaaggttcc gtcggggcgt ccggcccgca cgaatgccac
     3961 ctcgctctgg acgatcatgt cgtgaacgcc atcacccgtg acatcctcga actggagcga
     4021 tccgtccgag ctctcgtcca gcgtgctggc gtcggcgtcg atgaccagga gtggttcgag
     4081 cggccgtggt ccggagaggt cgaggaaggt cacgcctcga ttgcgatcga ggatcgacag
     4141 gagccgggag tcgccggtgg gtgcaaggaa cacgaactcc tggatccccg ccggccaacg
     4201 cagcacgcgt tgggatgtcc cgaacggcgg ccccgccgtc gccgtcgcgg ccaggaggcc
     4261 gatcaggacg atcggcagga gcttccgccc cgccggcctc atcggaccac cgtcacacgg
     4321 gtattggcgg tgccggaagg agaatcgagc cggacgaagt aggtgccggc cgggacaggc
     4381 gcacccgtca ggccgcagcc gctccactgg aggcagtgaa ggccggcggt ttccgggcgc
     4441 atggtggtgt cccagatccg tcgtccctgg acgtcgta
//
LOCUS       DASVOD010000636         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_353565, whole genome shotgun sequence.
ACCESSION   DASVOD010000636 DASVOD010000000
VERSION     DASVOD010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353565"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF720_13755"
     CDS             <1..884
                     /locus_tag="VF720_13755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7880474.1"
                     /translation="PLLRRTLASLEKQSPDTPAFEVIVIDDASPDDTPAFLAGYTGPL
                     TLRRVRQESNRGRAAARNAGLRIASGELIVFLDDDMEVVPGFVAAHVGFHGAGGVAAP
                     PGGRGRARAAIGNVTNAPEITDSPIIRYMSTRGAQKVRDGRPLPWRYFSTNNSSVPRV
                     DLEAVGLFDEEFRTYGFEDLELGYRLHRRGLSFGFLPGAESLHIHYHDLDDVLNKKEL
                     SGRSSLDIFIRKHPEAWREIPMSKWDSPGAKLVLNPVIYTMLKPLARFDAGPVSDRVF
                     DYLVLYRTLRGLRKARA"
     gene            881..1645
                     /locus_tag="VF720_13760"
     CDS             881..1645
                     /locus_tag="VF720_13760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880475.1"
                     /translation="MTMAVRIVTSGLKPHHRLDYVGIVRRLLTFVAPEHLAGIAMITI
                     SGDKPIEDDDDVLGQYFERWERDPAVIVVYLEPMKKEASAWIRRLPMAWEILIAQTLF
                     HEIGHHYQRFSHGIAKGRQEDHAELYGDRLARKAFPKAYRFIDWWRSVAGRWNRLMIR
                     RLVLETRFVPLARTHYEIGRRAWNEGDWETVVHHWEESLRLDPGASGVPRLLREARWQ
                     RAREDRKLQLTHAARVHPLRLKHRQARRRGGKKKRR"
     gene            complement(1715..2740)
                     /locus_tag="VF720_13765"
     CDS             complement(1715..2740)
                     /locus_tag="VF720_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880476.1"
                     /translation="MLASPFLVACGDDPVDPGPVPASALYVVNSLGETLDRIDLETGS
                     VSLAVMPLGNGPNDVVMTDPASADLAPARFWIANSLDNDVWGIDAAQLVPEPVIDLGN
                     NQNPYRLALLPDGRVAVTNWLGGTLALLDPVAGTVDDRRPIGRTPEAVLASGNRVFVT
                     AVGYDLPTGRFGPGKVYALDSSARGAGPADSAIVPTNPQALLIDTQGRLHVICTGNYG
                     GYEPASQGAVRVLNAATLDSVGAIALGATPGAGILVDDQIYLSAYFGGLLRYDANTLA
                     VTRGPDDPILDTPGLSGLAYDAGRHRLYVSGFEDDLVYVVDTTADTLVTAWEVGDGPI
                     ALGLALE"
BASE COUNT          495 a          979 c         1024 g          481 t
ORIGIN      
        1 tcccgctcct ccgtcgcaca ctggcatccc tggaaaaaca gtcccccgac acgccggcgt
       61 tcgaggtcat cgtcatcgac gacgcctcgc ccgacgacac gccggccttc ctggccggct
      121 acacggggcc gctcacgctc cgccgggtgc gccaggaatc gaaccgcggc cgcgcggccg
      181 cgcggaacgc cggtttacgg atcgcctccg gcgaactgat cgtctttctg gacgacgaca
      241 tggaggtggt gccggggttc gtggccgcgc acgtggggtt tcatggcgcg ggaggcgttg
      301 cggctccgcc cggcgggcgc ggccgggcgc gcgcggccat cggcaatgtc accaatgcgc
      361 cggagatcac cgacagcccg atcatccggt acatgtccac ccggggggcg cagaaggtcc
      421 gggacggacg gccgctccca tggcgctatt tttcgaccaa taattcgagc gtgccgcggg
      481 tcgacctcga ggcggtggga ctgtttgacg aagagttccg aacctatggg ttcgaggacc
      541 tggaactggg ataccggctt caccggcggg ggctgtcgtt cggcttcctc cccggggcgg
      601 agtcgctcca catccactac cacgacctgg acgacgtcct gaacaagaag gagctgtcgg
      661 ggcgatcgag cctcgatatc ttcatccgga agcatccgga ggcctggagg gaaattccca
      721 tgtcgaaatg ggattccccc ggggcgaaac tggtgttgaa ccctgttatc tatacaatgt
      781 tgaagccgct ggcccggttc gatgccgggc ctgtatcgga tagagtgttc gactacctgg
      841 ttctctaccg caccctccgc ggtctcagga aagcgagggc atgaccatgg cggtccggat
      901 cgtcacgtcg gggctcaagc cccaccatcg tctcgactac gtcggcatcg tccgccggct
      961 cctcacgttc gtggcccccg aacacctggc cggcatcgcc atgatcacca tttccggcga
     1021 caagccgatc gaggacgacg acgatgtcct cggccagtat ttcgaacgtt gggagcgcga
     1081 cccggccgtc atcgtggtgt acctcgagcc gatgaagaag gaggcctcgg cctggatccg
     1141 tcgcctgccg atggcctggg agatcctcat cgcccagacg ctgttccatg aaatcggcca
     1201 ccactaccag cgtttctcgc acggcattgc caaggggcgc caggaggatc acgccgagct
     1261 ctatggtgac cgcctggctc gcaaggcttt tccaaaggcc taccgattca tcgactggtg
     1321 gcggtcggtg gcggggcggt ggaaccggct gatgatccgc cggctcgtcc tggaaacgcg
     1381 cttcgtgccg ctggcccgca cgcactacga gatcggccgc cgtgcctgga acgaaggtga
     1441 ttgggaaaca gtcgtgcatc attgggagga gtcgctccgg ctcgatcccg gcgcctcggg
     1501 cgtgccgcgc ctcctgcgcg aggcgcgctg gcagcgggcc cgtgaggacc gcaagctcca
     1561 gttgacccac gccgcgcgcg tccacccgct tcgactgaag caccggcagg cccggcgccg
     1621 cggggggaag aagaagcggc gctgagggtt ggcgccgcgt tagcgacgac cggaccgcct
     1681 ccaactggtt ccggaacctg ttgattttcg ccgatcactc cagggccagt cccagtgcga
     1741 tggggccgtc gcccacctcc caggccgtga ccagcgtgtc ggccgtcgtg tcgacgacgt
     1801 agaccaggtc gtcctcgaag cccgacacgt agaggcgatg acgccctgcg tcgtacgcga
     1861 ggcccgacag gcccggggta tcgaggatcg ggtcgtcggg tccgcgtgtg acggcgagcg
     1921 tgttcgcgtc ataacgcagc agccccccga agtaggcact gaggtagatc tgatcgtcga
     1981 ccaggattcc cgcgccgggg gtggcgccga gcgcgatcgc gcccacgctg tcgagcgtgg
     2041 cggcattcag cacgcgcacc gctccctgcg aggccggttc gtaaccgccg tagttgccgg
     2101 tgcagatgac atgcaaccga ccctgtgtgt cgatcaggag cgcctgcggg ttcgtgggca
     2161 cgattgcgct gtcggccggg cccgcgcccc gggcggagga atccagggca tacacctttc
     2221 cggggccgaa tcgaccggtg gggagatcat agccgacggc cgtcacgaaa acgcggtttc
     2281 cggaggcaag gaccgcctcg ggcgtccggc cgatgggccg ccggtcgtcg acggtcccgg
     2341 cgaccggatc gaggagcgcc agcgtcccgc ccagccagtt cgtgacggcc acacggccgt
     2401 cggggaggag cgccaagcgg tagggattct gattgttgcc gaggtcgatg acaggttccg
     2461 gaaccagttg cgcggcgtcg attccccaga cgtcgttgtc gagcgaattg gcgatccaga
     2521 accgggcggg ggccaggtcg gccgaagccg gatccgtcat gacgacgtcg ttcggtccgt
     2581 tgccgagggg catgaccgcc aggctgacgg accctgtttc gagatcaatc cgatcgagag
     2641 tctcacccag cgaattgacc acgtagagcg ccgacgcggg caccgggccg gggtcgacgg
     2701 ggtcgtcgcc gcaggcgaca aggaacgggc tcgcgagcat cggtatgaca acgagtcgcg
     2761 acaggtgtgc aattcgtttc ggaacgagtc ggccgcgcgc gcgccgtggt tgcgctggcg
     2821 tcgcgatcct catcgcacga ccatcacgcg taccggtgcg gcccggccgt cgagcggacg
     2881 caaccagtag gcgcccgcgg gcaggcgacg cccggtgacg tccatcgggc gccaccaggc
     2941 ggattcggac gactccaggg taacgtggcc gtgcgaacg
//
LOCUS       DASVOD010000637         4110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_302973, whole genome shotgun sequence.
ACCESSION   DASVOD010000637 DASVOD010000000
VERSION     DASVOD010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302973"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF720_13770"
     CDS             <1..710
                     /locus_tag="VF720_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880477.1"
                     /translation="EKLTSIIILMMFLLSFGVEPLLAAAPRFESNLSRRSPGALRHLL
                     YIVRGTLAPATSPYAPASNPVRAAFRDLVLTLIVMVGIGVGVGGTSNWLNVITPHWLR
                     FDLLGVLIGTVVIVPGFYLLATRWRNLLQAMLGADKEGPGAERVRRIQRATRAALTTL
                     ALGLLGLAILPLALTALEAIRPVAIGLTVLVGLILAFFFRRALLRLHSLLGAGLTQRP
                     EATKTKQAGPDRPRVAQ"
     gene            757..2001
                     /locus_tag="VF720_13775"
     CDS             757..2001
                     /locus_tag="VF720_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880478.1"
                     /translation="MCHHWIRRTALAAGIIFLVAPAFADVAPPLDTVPFVEIDSPAGP
                     GSQSASLVAGKKAVYLTWIEKSDGAPVLKLSRNTGTGWSAPQTVLSDPRMVANAADVP
                     KFLALDNGMLVVAWLHGQGDAAEAYDVEVISSTDDGATWHSLGRPHADKARVEHGFVS
                     LLPHAGDGFWIAWLDGRNHANASHSPGVGQHGANTELRVARWRDGHFEAETKLDERVC
                     DCCQTAAVVTLEGLAVAYRDRGPKETRDISLVRYERQAWSKPIPIRNDNWVIDGCPVN
                     GPAMAAKDQNLALAWYTDANDQPQVFVALSDSSSFGLSRIVKVDDGRPMGRVDVEWLA
                     DQSALVTWVEQTGDATADIRVKRVTVDGTVSGSKTLATTGTGMSSGFPQLAARDIGIW
                     VAWTDPASHKVRLGHFDLMSTD"
     gene            complement(2011..2487)
                     /locus_tag="VF720_13780"
     CDS             complement(2011..2487)
                     /locus_tag="VF720_13780"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880479.1"
                     /translation="MERDRLALEIADAYDGDPWHGSPITAILDGIDATQAAARPIEDA
                     HTIWELVLHMTGWTREVARRVAGGKPGEPEAGDWPEVVDTSKSAWLAARRDLARTHEE
                     LRQAIKSLDDEAIMAPPRVPRDRAAGTGTTIYRSVHGLVQHDAYHAGQIALLKKLV"
     gene            complement(2535..3398)
                     /locus_tag="VF720_13785"
     CDS             complement(2535..3398)
                     /locus_tag="VF720_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX7880480.1"
                     /translation="MRGRLSAIEATRARSLDDLLDRLSAVPAPRLIAGGTDVMVQLEA
                     GAQKEGETFIDVSGLARELRYHRLDADALEIGALGTFRDTRARADIVSAFPLLELAAR
                     TIGATQTQTRGTWAGNIANGSPAADGVAALLAYDAEVIVASRGWRCVRSLTGFFSGYK
                     KMNLQAGEFIQAIRLPRRPPDRWSAFHKVGTRRAQAITKVGLTLNWRANGSWRIVGTS
                     LAPFVTRYETLEKLFEDAGDDLSRIDIHKLTAAVSAAVDPIDDIRSTADYRRKVVFNL
                     LRDASAHARAG"
     gene            complement(3395..3898)
                     /locus_tag="VF720_13790"
     CDS             complement(3395..3898)
                     /locus_tag="VF720_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX7880481.1"
                     /translation="MADTTTTFRVNGREVTVTGPPFRRLLDVLREELKLTGSKEGCGE
                     GECGACSAIVNGELVNTCIVPVLQAEGAVITTVEALADTPAGAAVQRAFMEHGGAQCG
                     ICTPGMIVAATWLAQQRASANHARIPAGEIREMLAGNLCRCTGYDLIVASVEAALASP
                     DDGSSRR"
BASE COUNT          642 a         1436 c         1364 g          668 t
ORIGIN      
        1 gcgaaaaact caccagcatc atcatcctga tgatgttcct cctgtcgttc ggggtggagc
       61 cgctcctcgc cgccgctccc cgcttcgaga gcaacctgtc gcgccgttcg ccgggcgcgt
      121 tgcgccacct cctgtacatc gtgcgcggca cgctcgcgcc ggccacctcg ccttacgcac
      181 cggccagtaa cccggtgcga gccgcgttcc gcgatctggt gctcacgctt atcgtgatgg
      241 tcggcatcgg cgtgggggtc ggcggtacct cgaactggct caacgtgatc acgccccact
      301 ggctgcggtt cgacctgctc ggcgtgctga tcggcaccgt cgtgatcgtg cccggcttct
      361 acctcctggc cacgcgctgg cgaaaccttc tccaggccat gctcggtgcg gacaaggagg
      421 ggccgggcgc cgaacgcgtc cggaggatcc agcgcgccac gcgcgccgcg ctcacgacgc
      481 tggccctcgg ccttctcggc ctggccatcc tgccgttggc gctcaccgcg ctcgaggcca
      541 tccggccggt ggcgatcggc ctcaccgtcc tcgtcggact catcctggcc tttttcttcc
      601 gacgcgccct gctccggctt cactcgctgc tcggggcggg cctcacccag cgacccgagg
      661 cgacgaagac gaagcaggct ggtcccgacc gtcccagagt ggcacagtga ttgcggcctc
      721 ctgaccctgg ccgcctccac ccgggagatt tttgccatgt gccatcactg gatccgccgg
      781 accgcgctcg cggccggaat tattttcctt gttgcgcccg ccttcgccga tgtcgcaccg
      841 ccgctcgaca cggttccctt cgtcgaaatc gactcgcccg ccgggccggg gagccaatcg
      901 gccagcctgg tggcggggaa gaaggccgtg tacctgacct ggatcgaaaa gtcggatggc
      961 gcgccggtcc tcaaactgtc gcgcaatacc ggcaccggat ggtcggcgcc acagacggta
     1021 ctgagcgacc cccgcatggt ggccaacgcg gccgacgtgc cgaaatttct ggcgctcgat
     1081 aacggcatgc tggtggtggc gtggctgcac ggccaggggg acgcggccga ggcgtacgac
     1141 gtggaagtga tcagctcgac cgatgacggc gccacctggc actccttggg caggccgcac
     1201 gccgacaagg cgcgcgtgga gcatggcttc gtctcgctgc tgccccacgc cggcgacggc
     1261 ttctggatcg cctggctcga cgggcgcaat cacgccaacg ccagccattc gccgggcgtg
     1321 ggccagcatg gcgccaacac cgagctccgg gtagcccgct ggcgtgacgg gcatttcgag
     1381 gccgaaacga agctggacga gcgcgtctgt gattgttgcc agacagcggc cgtcgtcacg
     1441 ctcgaggggc tggcggtggc gtatcgcgat cgcggcccga aggaaacgcg cgacatctcg
     1501 ctggtgcgct acgagcgcca ggcctggagc aagccgatcc cgatccgcaa cgacaactgg
     1561 gtgatcgacg gttgcccggt gaacggcccg gccatggccg cgaaagacca gaacctggcc
     1621 ctggcctggt acaccgacgc caacgaccag ccgcaggtgt tcgtggcgct ctccgactca
     1681 tcgtcgttcg gcctgtcgcg catcgtgaag gtggacgacg gccgcccgat gggacgcgtg
     1741 gacgtggagt ggctggccga ccagagcgcc ctcgtcacct gggtggaaca gaccggcgat
     1801 gccaccgccg acatccgcgt gaagcgcgtg acggtggacg gcacggtttc gggttcgaag
     1861 acgcttgcca cgacgggcac cggcatgtcg agcggctttc cccagcttgc cgcccgcgac
     1921 atcggcatct gggtggcgtg gaccgatccc gcgtcccata aggtgcggct cggccacttc
     1981 gacctgatgt cgaccgacta gggcgccccg ctagacaagc ttcttgagca gcgcgatctg
     2041 tccggcgtga taggcgtcgt gctgcacgag gccgtgcacc gaccggtaga tcgtcgtccc
     2101 cgtgccggcg gcgcgatcgc gcggcacgcg gggcggcgcc atgatggcct cgtcatccag
     2161 cgacttgatg gcctgcctga gttcctcgtg ggtcctggcg agatcgcgcc gggccgccag
     2221 ccacgcggac ttcgaggtgt cgacgacctc gggccagtcg ccggcttcgg gctcgcccgg
     2281 cttgccaccc gccacccgcc gggccacttc gcgggtccag ccggtcatgt ggaggacgag
     2341 ctcccagatc gtgtgggcat cttcgatggg gcgggcggcc gcttgagtcg cgtcgatccc
     2401 gtcgaggatg gcggtaatcg gcgaaccgtg ccagggatcg ccgtcgtaag cgtcggcaat
     2461 ctccagggcc agtcgatcgc gttccatgaa atgaaaacct ccggggggca ggcagtcggt
     2521 gatcgcgcgc gccgtcagcc ggcgcgcgca tgcgcgctgg catcgcgcag gagattgaag
     2581 acgaccttgc gccgatagtc ggcggtggag cggatgtcgt cgatgggatc gacggccgcg
     2641 gaaaccgcgg ctgtcagctt gtggatgtcg attcgagaga ggtcgtcacc cgcatcctca
     2701 aacagtttct cgagcgtttc gtaccgcgtc acgaacgggg cgaggctcgt acccacgatc
     2761 cgccacgagc cgttcgcacg ccagttcagc gtgaggccca ctttcgtgat ggcctgggca
     2821 cgccgcgttc ccaccttgtg gaacgcggac cagcgatcgg gaggtcggcg cggcagtcga
     2881 atcgcctgga tgaattcccc cgcctggaga ttcatcttct tgtatccgga aaagaatccg
     2941 gtcaatgacc tcacgcaccg ccacccgcgg gaggccacga tcacctcggc gtcgtacgca
     3001 aggagcgccg ccacgccatc cgccgccggc gatccgttgg cgatgttgcc cgcccaggta
     3061 ccgcgggtct gggtctgcgt ggccccgatg gtacgggcag cgagttcaag gagcgggaat
     3121 gccgagacaa tgtcggcccg agcccgcgta tctcgaaaag tcccaagcgc gccgatctcg
     3181 agcgcatcgg catccagccg gtgatagcgc agttccctcg ccaggcccga cacgtcgatg
     3241 aaggtttcgc cttccttctg cgcaccggct tccagctgca ccatgacatc cgtgccgccc
     3301 gcgatcagtc gcggcgccgg cacggcggac aagcgatcga gcaggtcgtc gagggatcgg
     3361 gcccgggtag cctcgatggc cgacagccgg cccctcatcg ccgggacgac ccgtcgtcgg
     3421 gcgacgcgag cgccgcctcc acgctggcca cgatcaggtc gtagccggtg cagcggcaga
     3481 ggttgcccgc cagcatctcg cgtatttcgc cggccggtat gcgggcgtgg tttgccgagg
     3541 cacgctgctg cgccagccag gtggccgcca cgatcattcc cggcgtgcag atgccgcact
     3601 gggccccgcc atgctccatg aacgcgcgct gcaccgccgc gccggccggt gtgtcggcca
     3661 gggcctccac cgtcgtgatg accgcccctt cggcctggag gaccggcacg atgcaggtgt
     3721 tcacgagttc gccattcacg atggcactgc aggccccgca ttccccctcg ccgcaccctt
     3781 ccttgctgcc ggttagcttg agctcctcgc gcagcacgtc gagcaggcgc cggaagggtg
     3841 gaccggtgac ggtcacctcg cggccgttca cgcggaaggt ggtggtcgtg tcagccacgc
     3901 gccgcctcca tcgagtcggc cagcgcgcgt gaccagcacc agaagagttc ctccggaatc
     3961 ttaagcggca agactaggca aaccgacgac gccagcagct acagcaggca aaacggcagc
     4021 accttcagtg gcaacaccac cgtgctcgcc gccggcaacg acgccaccat caccggcagc
     4081 gacgtcgttt ccacgcaagg tacgcagatc
//
LOCUS       DASVOD010000638         2467 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_562955, whole genome shotgun sequence.
ACCESSION   DASVOD010000638 DASVOD010000000
VERSION     DASVOD010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562955"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VF720_13795"
     CDS             <1..666
                     /locus_tag="VF720_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880482.1"
                     /translation="YGNSEPSWWPAPANVVEYDIDPDTGRLLEEGCRPRWGNTSTEIF
                     IRGTEPESICPESDYEYTWFNVSGSGDDQEGGDGYESDEEARQGRRDDDERHDEDAPE
                     IDLDGGWIFELGINDTNDRNLRNYTATWQVRLWVDGNDVHGSGEKIAEDGRRVDNDRR
                     TSISLSGQVNGESLRLRLTEYNGEGALSGWFNVDLEGRRLRGEFVSSDAATRGAVSAR
                     RSD"
     gene            complement(718..1782)
                     /locus_tag="VF720_13800"
     CDS             complement(718..1782)
                     /locus_tag="VF720_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880483.1"
                     /translation="MPNASSLRRLAAALAFALAGCSHETPTTTPLGREADDPPATTAV
                     SLLEDFSNRQVFPIDNWWNQDVSMAPLDPNSNAIVTWINNPSPANPNRRQRLHPDFGP
                     PPYGIPYVGVDGTQPRVAVTFVLYGSQSDAGAPGFPSGYPIPIECRTQPNYIEGGVIG
                     GGSSGDRHLLIIDRDNNLLFETWATRWNSSLSRWEAGSGAIFDMDTNNRRPDGWTSAD
                     AAGLAIFPGLIRRDELVAAGEIEHAFRFTTRATNGYVWPASHEAGNDPAAPPMGMRMR
                     MKASKVITGYTPELQKIFRAMKTYGLILADNGTDMYIQGTMDPGWDNDILNPAFHGLY
                     ADDFEVIQLGWQPSTTGVPE"
     gene            1946..>2467
                     /locus_tag="VF720_13805"
     CDS             1946..>2467
                     /locus_tag="VF720_13805"
                     /inference="COORDINATES: protein motif:HMM:NF033196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX7880484.1"
                     /translation="MPRPSLRSSLILAGVLVTRISLQAALATSQTPAGTPPGGPPPPP
                     PPPQNLQFYPKDMPRPELIARMREFSFALGVGCEYCHVDEHEGPNPREDFATDEKPAK
                     VKARAMLVMTKEINDDLMDKIPHRVTPGVNVSCATCHHGLPVPQTLTERLTAAAAAGG
                     ADSVDADLTMLHTE"
BASE COUNT          411 a          830 c          829 g          397 t
ORIGIN      
        1 tacggcaact cggaaccctc gtggtggccg gcgcccgcca acgtggtgga gtacgacatc
       61 gatcccgaca ccggccgcct cctcgaggaa gggtgccgcc cccgctgggg caacacgtcc
      121 acggagatct tcatccgcgg caccgaaccc gagtcgatct gtcccgaatc cgactacgaa
      181 tacacctggt tcaacgtgag cggcagtggt gacgaccagg agggcggcga cggctacgag
      241 agcgacgagg aagcacggca gggtcgccgc gacgacgacg agcgccatga cgaggacgct
      301 ccggaaatcg acctcgacgg tggctggatc ttcgagctgg gcatcaacga cacgaacgac
      361 cgcaacctgc gcaactacac cgccacctgg caggtgcgcc tctgggtgga cggcaacgac
      421 gtccacggca gcggcgaaaa gatcgccgag gacgggcgcc gggtggacaa cgatcgccgc
      481 acgtccatct cgttgtcggg ccaggtgaac ggcgagagcc tccgcctgcg cctcaccgaa
      541 tacaacggcg agggcgcgct gagcggctgg ttcaacgtgg atctcgaagg gcgccgcctg
      601 cgcggcgaat tcgtgagctc cgacgccgcc acacgcggtg cggtgagcgc gcggcgcagc
      661 gactgacgcc cggcttcctt tgcgggcaac tggtcccgcg accaggcggg gtttcgccta
      721 ctccggaact cccgtcgtgg acggctgcca gccgagctgg atcacctcga agtcgtccgc
      781 gtagaggccg tggaaggccg ggttcaggat gtcgttgtcc cagccgggat ccatcgttcc
      841 ctggatgtac atgtcggtgc cgttgtcggc caggatgagg ccgtacgtct tcatggcccg
      901 aaagatcttc tgcagctcgg gcgtgtaacc cgtgatcacc ttgcttgcct tcatccgcat
      961 ccgcattccc atcggcggcg ccgccgggtc attgcccgct tcatgcgagg ccggccacac
     1021 gtagccgttg gtggcgcgcg tcgtgaatcg gaacgcgtgc tcgatctcgc cggccgccac
     1081 gagctcgtcg cgacggatca atcccggaaa gatggccagc cccgccgcgt cagccgaggt
     1141 ccagccgtcg gggcgccggt tgttggtgtc catgtcgaag atggcaccgg agccggcttc
     1201 ccagcggctc agcgaggaat tccagcgcgt ggcccacgtc tcgaagagga ggttgttgtc
     1261 gcgatcgatg atcaggagat gtcggtcgcc cgaactccca cccccgatca cccctccttc
     1321 gatgtaattg ggttgcgtgc ggcactcgat cgggatggga tagccgctcg ggaagccggg
     1381 cgcgccggca tcgctctggc tgccgtacag cacgaaggtc accgccacgc gcggctgggt
     1441 gccgtccacg cccacgtagg gaatgccgta agggggcggc ccgaaatcgg ggtggagtcg
     1501 ctgccgccgg ttcgggttgg ccggcgaagg attgttgatc caggtgacga tggcgttcga
     1561 gttcggatcg agtggcgcca tcgacacgtc ctggttccac cagttgtcga tggggaagac
     1621 ctgccggttg ctgaagtcct cgaggagcga aaccgcggtc gtggccggcg gatcgtcggc
     1681 ctcgcgaccg agcggggtgg tggtgggggt ctcgtgcgaa cagccggcga gggcgaaagc
     1741 gagtgcggcg gccagtcgac gcagtgacga cgcgttcggc atggtgtggc tccttggatc
     1801 gcgatgcgca tctgggcagc ggccgtccga tgtggtccct cccagtatcg gcccatccct
     1861 cggcgaacgc cacctcccgc gtgctaggat gccgcgtttc ctgaccgatt tccgtgattc
     1921 agggtcctgt cacctgggag aaccgatgcc ccgcccatcc ctccgttcct ccttgatcct
     1981 ggccggggtc ctcgttaccc gcatctcgct ccaggccgct ctcgccacct cgcaaacgcc
     2041 ggccggaact cccccgggtg ggccacctcc cccgccgccg ccgccccaga acctccagtt
     2101 ctatcccaag gacatgcccc gccccgaact gatcgcccgg atgcgcgagt tctccttcgc
     2161 gctcggggtg ggatgcgagt actgccacgt ggacgagcac gaggggccga acccgcgcga
     2221 ggactttgcc accgacgaga agccggccaa ggtgaaggcg cgggccatgc tggtcatgac
     2281 gaaggaaatc aacgacgacc tgatggacaa gatcccgcac cgcgtgacgc ccggcgtgaa
     2341 cgtgagctgc gccacctgcc accacggact gccggtgccg cagacgctca ccgagcggct
     2401 gaccgcggct gctgcggccg gtggtgccga ctccgtcgac gctgatctca ccatgctgca
     2461 caccgaa
//
LOCUS       DASVOD010000639         4316 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_303036, whole genome shotgun sequence.
ACCESSION   DASVOD010000639 DASVOD010000000
VERSION     DASVOD010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303036"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VF720_13810"
     CDS             <1..509
                     /locus_tag="VF720_13810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880485.1"
                     /translation="AYTPGTTSLTKHPETNQNTVTDCGGVVDVLPSIDDPGCTWLGTV
                     AFGGFSGVNPCGRGAGAPGCQPIGSCSFDGPAVVTAGQTGIRYDGGAVNATNTWSITG
                     NGTIVGSTHDLHVIVDAGAPGSFTLRLRFSYGPQDLSECVQVIPVQDPVPAVPLSWGR
                     IKNLSVSR"
     gene            664..1650
                     /locus_tag="VF720_13815"
     CDS             664..1650
                     /locus_tag="VF720_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880486.1"
                     /translation="MRSSLRSVPGVIFLLFAAGQADPAGAAGNRNAKIMLQALSPVMK
                     NGCVRPATVPATCSGYVVNNLGLYPTRHFVYALVTNGTQVEGVSGAAFGIDYDPALQS
                     GVDVFSWTLCATLEFPSAGWPAAGGGNLITFDAVTRCQTSGNANVGVVAVLGYFYCAA
                     YTPDVMRVTRRPVYDDASVTNCGLEVDETPSGNEGPWCNFLGQLGFGPGRTGHNPCGL
                     EIIDVACGSPVSCAITGPDDVTSGQTGIPFQYTGGVGDPRWTITGNGTFDGPTYNQHT
                     VYVTAGSPGQFTVTLHTQAGHGSADCERTITVEDAVPAVPTSWGRIKALVRD"
     gene            complement(1647..1907)
                     /locus_tag="VF720_13820"
     CDS             complement(1647..1907)
                     /locus_tag="VF720_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880487.1"
                     /translation="MNDTSPEIWQLFCSMLRELPDAQRVRMGFGYSAFTRRLIREGLA
                     REGLPTNGVEFARRLYPDLTSDVIESLRPQGAPLLLPLSGAD"
     gene            complement(2024..2299)
                     /locus_tag="VF720_13825"
     CDS             complement(2024..2299)
                     /locus_tag="VF720_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880488.1"
                     /translation="MRMEDLEVVRAAVVSRLDGGQIPYMISGTIAANCYGVPRSTADP
                     NLVVQLAPGDVARIVRLFDDEFVVSADAVREAVTHPLQVALVKQHPL"
     gene            complement(2671..3651)
                     /locus_tag="VF720_13830"
     CDS             complement(2671..3651)
                     /locus_tag="VF720_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7880489.1"
                     /translation="MGVVDTKPATGGKWEMLLTRQAYADALVELGEEDPRVVVLDADL
                     AQSTLTRFFGEKYPHRFFEMGISEQDMVGTAVGLSHMGFIPFLSSYSIFLSGRAWDQV
                     RNGVAYPSANVKIAAAHGGISVGKDGPTHQSMEDVGVIRPIVNFTVIVPCDYHETFKT
                     TKWSAHHEGPVFFRMGREKVPVITTKDTPFEPGKAIVMEDGPDGTIIACGLMVAQALE
                     AAEILKKKGIRPRVLNMHTIKPLDHGAVLAAARETGAIVTAEEHSRYGGLGSAVAEVI
                     VESDYRVPMRIIGVNDRYLESGPPEDLMQLAGLTPQKLAEALEGALKKGR"
     gene            complement(3651..>4316)
                     /locus_tag="VF720_13835"
     CDS             complement(3651..>4316)
                     /locus_tag="VF720_13835"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX7880490.1"
                     /translation="FPPKWLLRFRMSGSPLQGHPNMIYVPGLEASTGSLGQGLSQANG
                     MALGARLKGHDSVRIYCNCGDGELQEGNIWEAVMTAAHYKLDNMCLLVDFNNAQIDGR
                     VEDVMGIEPLGDKFRAFNFHVIETDGHDYQKIAAAYDEARDTKGKPTVILFRTLMMKG
                     VPAYEDNFKWHGKPLNDDELAIAMQALGFQGTKADLVATYEDWEKQPPKPGPIWAGAG
                     AHH"
BASE COUNT          754 a         1375 c         1408 g          779 t
ORIGIN      
        1 cggcatatac gcccgggacc acgtcactta ccaaacaccc tgagaccaac cagaacacgg
       61 tgacggactg cggtggcgtg gtggacgtac tcccctcgat cgatgacccc ggctgcacct
      121 ggctcgggac cgtggccttc gggggcttca gcggcgtcaa tccatgcggg cgcggtgccg
      181 gggcaccggg ctgccagccc atcggaagct gctccttcga tgggcccgct gttgtgaccg
      241 cggggcagac cggcatccgt tacgacggtg gcgcggtcaa cgccacgaac acctggagca
      301 tcaccggaaa cgggacgatc gtcggcagca ctcacgacct gcacgtcatc gtcgacgccg
      361 gggcccccgg ttccttcaca ttgaggctga ggttctccta cggtccgcaa gacctgagtg
      421 agtgcgtgca ggtgatcccc gtccaggatc cggtgccggc ggttccgttg agctgggggc
      481 gaatcaagaa cttatcggtg agcaggtgac aatcggatgc ctggcgctcg agacatgaag
      541 gaccagccgc agcggcaggc cgtatagagt agaagcaact tccctgccgc cagttgtaaa
      601 atgcgacaaa gggcgccaag ccccggccct gcacagtttg gcatgacacc ggagaaatga
      661 accatgcgat cgtcattacg ctcggttccg ggtgtgatct tcctcctgtt cgccgccggc
      721 caggcggatc ctgccggtgc cgccggcaac cgaaacgcca agatcatgct ccaggcgttg
      781 tcgccggtga tgaagaacgg gtgcgtgcgg cccgcgaccg taccggctac gtgcagtggc
      841 tacgtcgtga acaatctggg cctgtatccg accaggcact ttgtgtatgc gctggtcacg
      901 aatgggacac aggtcgaggg tgtttccggg gcagcctttg gcatcgacta cgatccggcc
      961 ctgcagtccg gcgtcgatgt cttcagctgg acgctctgcg ccaccctcga atttccttcg
     1021 gccggatggc cggcggccgg cggcggcaat ctcatcacct tcgacgccgt gacgcgctgc
     1081 cagacatccg gaaacgcgaa tgtcggcgtg gtggccgtcc tgggctactt ctactgcgcg
     1141 gcctacacac ccgacgtcat gcgcgtcacg cggcggccgg tctacgacga cgcctccgtg
     1201 accaactgcg ggctggaggt cgacgaaacg ccgagcggca atgaagggcc gtggtgcaat
     1261 ttcctgggtc agctcggttt cggacccggc cgaacgggtc acaatccctg cggtctcgag
     1321 atcatcgacg tcgcgtgcgg cagtccggtc agctgcgcca tcacgggacc ggacgatgtc
     1381 acttcaggac agacggggat cccgttccag tacaccgggg gggtcgggga tccccggtgg
     1441 acgatcaccg gcaacggcac cttcgatggc ccgacgtaca accagcacac cgtttacgta
     1501 accgccggtt ccccgggcca gttcaccgtc acgctgcaca cccaggccgg ccatggcagc
     1561 gccgactgcg aacggaccat caccgtggag gatgccgtgc cggcagtgcc gacgagctgg
     1621 ggacggatca aggcgctggt gcgggactag tcggctcctg acaggggcag gagcaggggt
     1681 gcaccctgcg gacggagtga ctcgatcacg tcactcgtga gatccggata gaggcgcctg
     1741 gcgaattcca cgccgttcgt cggcaggcct tcccgagcca gtccttccct gatgagccgg
     1801 cgggtgaagg cggaatagcc gaatcccatc cggacgcgct gggcgtccgg caactccctc
     1861 agcatgctgc agaagagctg ccagatttcg ggagatgtgt cattcacggc gggcctccat
     1921 cagtcgatcg tgggtgccca tccggcggga ccaccgatcc atgtatgcct cgtccagccc
     1981 cgcctggacg gccaacagcc cacgaatgtc acgcatctgc atttcagagg gggtgctgtt
     2041 tgaccagagc aacttgtaga ggatgagtga ccgcctcgcg gaccgcatcg gccgagacga
     2101 caaactcatc gtcgaagagc cggacgatcc gtgccacatc gcccggagcg agctggacga
     2161 cgaggttcgg gtccgccgtg gaacggggaa cgccgtagca gttcgcggcg atggttccgc
     2221 tgatcatgta ggggatctga ccaccgtcca accgggatac gacggcagcc cggacaactt
     2281 cgaggtcttc cattctcact ttcgagcatc tccacggggg cgccgtgaga tgggggctct
     2341 ggggtgcgca ggtcgacaac gggttcgtca tcgactgtgg gagaccgtgc gccgacaggc
     2401 gccaagtcgt caagtgcgag attggcagag cctgggcgtc cgccatcttc gggcaaggga
     2461 tcccgcacac acaatcacgc gatgtcttac attgtaagac agtgtgcgat tcgaacaacc
     2521 gcagggtccc gtgatgtccg atccagtcga tgttggctgg tcccggtctc gagggaacgt
     2581 cgattctgac attgaaacag aatggccaac tcggtccaaa ccgagttggc catcaatgta
     2641 tccatatcag gttgggattc gatcggccga tcaacgcccc ttcttcaacg ccccctccag
     2701 cgcctccgcc aatttctgcg gcgtgagccc ggcgagctgc atcaaatctt cgggcgggcc
     2761 gctctcgaga taccggtcgt tcacgccgat gatgcgcatg gggacgcggt agtcgctctc
     2821 cacgatgacc tcagccacgg cggagccaag accaccgtag cgcgagtgct cctcggcggt
     2881 gacgatcgcc cccgtctccc gcgcggcagc cagcacggca ccgtgatcca gcggcttgat
     2941 cgtgtgcatg ttgagcacgc gtggccggat acctttcttt ttcaggatct cggcggcttc
     3001 gagcgcctgg gccaccatga gcccgcaggc gatgatcgtg ccgtcggggc cgtcttccat
     3061 cacgatggcc ttgccgggct cgaacggggt gtccttggtc gtgatgacgg gcaccttttc
     3121 gcgccccatc cggaagaaga cgggtccctc gtggtgcgcg gaccacttcg tcgtcttgaa
     3181 cgtctcgtgg tagtcgcacg gcacgatgac ggtgaagttg acgatgggac ggatgacgcc
     3241 cacgtcctcc atcgactggt gcgtcgggcc gtccttcccc accgagatgc cgccgtgcgc
     3301 cgcggcaatc ttcacgttgg cgctgggata ggccacgccg ttccgcacct ggtcccaggc
     3361 gcggccggag aggaagatcg agtagctcga gaggaacggg atgaatccca tgtgggagag
     3421 gccgaccgcc gtccccacca tgtcctgctc ggagatcccc atctcgaaga agcgatgggg
     3481 gtacttctcg ccgaagaaac gcgtgagcgt ggactgcgcg aggtcggcat ccagcacgac
     3541 gacgcgcggg tcttcctcgc ccagttcgac cagcgcatcg gcgtaggcct ggcgggtgag
     3601 gagcatctcc cacttgccgc cagttgcggg tttcgtgtca acgacgccca tcagtggtgc
     3661 gctcccgcgc cggcccagat ggggccgggt ttggggggct gcttctccca gtcttcgtag
     3721 gtggccacga ggtcggcctt cgtgccctgg aagccgaggg cctgcatggc aatggcgagc
     3781 tcgtcgtcgt tgagcggctt gccgtgccac ttgaagttgt cttcgtacgc cggcaccccc
     3841 ttcatcatca gggtgcggaa gagaatgacc gtgggcttcc ccttggtgtc cctggcctcg
     3901 tcgtacgcgg cggcgatctt ctggtagtcg tggccgtcgg tctcgatgac gtggaaattg
     3961 aacgcccgga acttgtcgcc caaaggctcg atccccatga cgtcttccac gcggccgtcg
     4021 atctgggcgt tgttgaagtc gaccaggagg cacatgttgt cgagcttgta gtgggcggcc
     4081 gtcatgaccg cttcccagat gttcccttcc tggagctcgc cgtcgccgca gttgcagtag
     4141 atgcggaccg agtcgtgccc cttgagccgc gcgcccagcg ccatgccgtt ggcctgcgag
     4201 agtccctggc cgagcgagcc ggtcgaggcc tcgagccccg gcacgtagat catgttggga
     4261 tgaccctgga ggggcgagcc cgacatccgg aaccgcagga gccacttcgg cgggaa
//
LOCUS       DASVOD010000640         2915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_303048, whole genome shotgun sequence.
ACCESSION   DASVOD010000640 DASVOD010000000
VERSION     DASVOD010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303048"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2780)
                     /locus_tag="VF720_13840"
     CDS             complement(<1..2780)
                     /locus_tag="VF720_13840"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX7880491.1"
                     /translation="MNDWLEAASSGWTPNLGQVATSDGQVARDVLYTTATPGGRVYVT
                     RTGLTHLFVVGSEDYAAREESDHDENENRVAEPGQLPEASSYEWARVDVSLVGAAIRP
                     ELADARGRIDAEGTQNFYLAHCPDGVLDVPAYSEVSFPGVYPGVDWLVRNAPGDGVHH
                     DFVVAPGADPGAIRLRYEGATALRVSDDGQSLIIRTALGDVREGSLFCHQGNLSEPVS
                     ARFELAGNEVSVRVGNYDRSKPLVIDPPLVWSTYYGGTLYDGPRSIWCDNANAVIYVV
                     GYTGSFDLPAFNPGGGTYYQGTSGGARDAFLWKFSQSGQRLWATYYGGSADEGFSDCA
                     LDAAGNLYVGGTTTSTNLPVQVLGGAFNDGSLGGIIDGYFVKFNPSGVRLWASYIGGS
                     DNESLEGVAVDAGGQLQVCGFTMSTNYPLTNPGGGAYFQPSPASTTDAFLSRFSATGM
                     MNWSTYLGGNDDDYAHGIITSPSGNIFVTGMTVSTTFPVLTLGGAYNQGANGGGQDGF
                     IARFSSGGVQQWTTYYGGSGNDIGDEPALDASGNLYFYGMTSSTDFPTLNSGGAYYQG
                     TYGGGGFDLFLIRFNGAATRTWGTYIGGNGIEFLNGPGGKPITVDNLGRVIITGLTDS
                     SNYPLQNLGGSYFLGSCQGQQDAIITRFNTNMSMNWSTYWGTTSFDFGTSVATNDDAC
                     IFATGESVELGSLVNVTPGFWAWNQPANAGSDDGYIAKFCSASSACCIDFTCVPVSSA
                     AECNSLGGTVFHSGQPCSTVVCTVNCTICGTKFNDLNRDGVKQGNEPGLPGWTIQLNY
                     PGGAPYATTTTDGMGNYCFVGVPCGPWEVAEVHQPGWVQIYPGGPGHTLNTTTGTTTS
                     GVNFGNYECLTPPPCATPPTGLAARWVFGDDPVTGVALDVAHTEPVRNAATLTTGGSV
                     PDLLCLSTPADRA"
BASE COUNT          479 a          933 c         1037 g          466 t
ORIGIN      
        1 gcccggtcgg ccggcgtgct gaggcagagg aggtcgggca cgctgccgcc cgtggtgagg
       61 gtggccgcat tgcgcaccgg ctcggtgtgg gccacgtcga gggccacgcc ggtgacgggg
      121 tcatccccga atacccagcg cgcggcgagg ccggtcggcg gcgttgcgca ggggggcggg
      181 gtgaggcatt cgtagttgcc gaagttcacg ccgctggtgg tcgtgccggt cgtggtgttg
      241 agcgtgtgcc ccgggccgcc ggggtaaatc tgcacccagc ccggctggtg cacctcggcc
      301 acttcccacg ggccgcacgg cacgcccacg aagcagtagt tccccatgcc gtcggtggtg
      361 gtggtggcgt acggcgcgcc accgggatag ttgagctgga tcgtccagcc gggaaggccc
      421 ggctcgttcc cctgcttcac gccgtcacgg ttcaggtcgt tgaacttcgt gccgcagatc
      481 gtgcagttga ccgtgcacac caccgtcgag cagggctggc cggagtgaaa cacggtgcct
      541 cccaggctgt tgcactcggc ggcgctcgaa accggcacgc aggtgaagtc gatgcagcag
      601 gcagaggatg ccgagcagaa cttggcgatg tagccgtcgt cggaaccggc gttggccggc
      661 tggttccagg cccagaagcc gggcgtcacg ttgacgaggg agccgagctc caccgactcg
      721 ccggtggcga agatgcaggc atcgtcgttg gtggccacgc tggtgccgaa atcgaacgag
      781 gtcgtgcccc agtaggtgga ccagttcatg ctcatgttcg tgttgaagcg ggtgatgatg
      841 gcgtcctgct gtccctggca actccccagg aagtacgagc cgccgaggtt ctggagcggg
      901 taattcgacg agtcggtgag gccggtgatg atcacccggc ccaggttgtc caccgtgatc
      961 ggcttgccgc ccggaccgtt caggaattcg atgccgttgc cgccgatgta cgtcccccag
     1021 gtgcgtgtgg ccgcgccgtt gaatcggatg aggaagagat cgaatccccc tccgccatag
     1081 gtaccctggt agtacgcgcc gcccgagttg agcgtgggga agtcggtcga cgaggtcatt
     1141 ccgtagaagt agaggttgcc cgaggcgtcg agagccggct cgtcgccgat atcgttgccg
     1201 gagccgccgt aatacgtggt ccactgctgc acgccgccgc tcgagaagcg ggcgatgaag
     1261 ccgtcctggc ccccgccgtt cgcaccctgg ttgtacgccc cgcccagcgt caggaccggg
     1321 aaggtcgtgg acaccgtcat gccggtcacg aagatgttgc ccgacgggct ggtgatgatc
     1381 ccgtgggcgt agtcgtcgtc gttgccgccg aggtacgtgg accagttcat catcccggta
     1441 gcgctgaagc gcgagaggaa ggcgtcggtg gtcgaggccg gcgagggctg gaagtaggcg
     1501 ccaccgcccg ggttcgtcag cggatagttg gtggacatcg tgaagccgca cacctggagc
     1561 tggccgccgg catccacggc cacgccttcc agtgattcgt tgtcggagcc gccgatgtaa
     1621 ctggcccaga ggcgcacgcc ggacggattg aacttgacga agtagccgtc gatgatgccg
     1681 ccgagcgagc cgtcgttgaa ggcgcctccc agcacctgca ccggcaggtt ggtggaggtg
     1741 gtggtgcccc ccacgtagag gttgccggcc gcatcgagcg cgcagtcgga gaacccttcg
     1801 tccgcgctgc cgccgtaata cgtggcccag aggcgctgtc ccgattgcga gaacttccag
     1861 aggaaggcat cgcgagcccc gcccgaggtg ccctggtagt aggtgccgcc tcccggattg
     1921 aaggcgggga gatcgaacga gccggtatac cccaccacgt agatgacggc attggcgttg
     1981 tcgcaccaga tcgagcgagg gccgtcgtac aacgtgccgc cgtaatacgt ggaccagacc
     2041 agcggcggat cgatgaccag cggcttcgaa cggtcgtagt tgccgacccg cacgctcacc
     2101 tcgttgccgg ccagctcgaa ccgggcggac accggctcgg acaggtttcc ctggtgacag
     2161 aagagcgagc cctcgcgcac atcgccgagc gcggtccgga tgatgagcga ctggccatcg
     2221 tccgaaacgc gcaacgccgt ggcgccctcg tagcggagcc ggatggcgcc cggatccgcg
     2281 ccgggcgcca cgacgaaatc gtggtgcacc ccatcgcccg gtgcgttccg cacgagccag
     2341 tccacgccgg gatacacgcc ggggaacgac acttcggaat acgccggcac gtcgagcacg
     2401 ccgtcggggc aatgggcgag atagaagttc tgcgtcccct ccgcgtcgat ccggccgcgc
     2461 gcatcggcca gctccggccg tatcgccgct ccgaccagcg acacgtcgac acgcgcccac
     2521 tcgtagctgg aggcttccgg caactgcccg ggctcggcca cgcgattctc gttctcgtcg
     2581 tgatcggact cttcccgcgc cgcgtaatct tcgctgccga ccacgaaaag atgggtgagg
     2641 ccggtgcgcg tcacgtacac ccggccgccg ggcgtcgcgg tcgtatagag gacatcgcgg
     2701 gccacctggc cgtcggaagt ggccacctgg ccaagattcg gcgtccatcc ggaagacgcg
     2761 gcctcaagcc agtcattcac ccgggcttcg gtggcggtgt cggggcgatg ggcccccgcc
     2821 gggacgcctg ccggtgcggg ggccgcgatg gcggccggga ccagggctga caagctgaga
     2881 acgaacagga tggggaatac cgcaatccgg gggga
//
LOCUS       DASVOD010000641         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_303115, whole genome shotgun sequence.
ACCESSION   DASVOD010000641 DASVOD010000000
VERSION     DASVOD010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303115"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF720_13845"
     CDS             <1..215
                     /locus_tag="VF720_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880492.1"
                     /translation="GGFDLEVLEDATAPENLLKERGRGIFIMRSMMDDVSFDFTDKGT
                     RCRLVKRRRVGEADVSGGPAPLDDTL"
     gene            199..672
                     /gene="ruvX"
                     /locus_tag="VF720_13850"
     CDS             199..672
                     /gene="ruvX"
                     /locus_tag="VF720_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX7880493.1"
                     /translation="MTTRSDDRILAVDPGTKRIGLAVASVTQGMASRLAVLDVQPGAS
                     GLERAVRAVARHAAEEEATRILVGLPLHLDGREGKEARAARRLGLALAEATGLPVDFA
                     DERLTSEAAIEEARKSGWTPKSKKPIDDLAAALLLQGWLDDASARAARQRLNEST"
     gene            669..1700
                     /gene="mltG"
                     /locus_tag="VF720_13855"
     CDS             669..1700
                     /gene="mltG"
                     /locus_tag="VF720_13855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX7880494.1"
                     /translation="MTTAVKNLLWIAALFTFLAAGVASLFMPASLTPGDPAIRITLSP
                     GMTTRQIGATLRRSGLIRSSDAFMLVAHLSGRASQLRAGTYAISASEGIGRIVDRLAD
                     GDVLRVAVTIPEGFNAREIAARVAPVLGCPVEEFMAIVSDSVMAESLGVGEGTLEGYL
                     FPDTYNVIPGTDPAGFIARLVERTTTLFEERYRARADSLGLARHQALTLASLVEAEAA
                     VDEERPRISAVFWNRLREGMMLQSDPTVAYALGRRPDRIYYKDLEVQSPYNTYLVTGL
                     PPGPICSPGEASIRAALHPVPGSKELYFVATGDGHHLFSTTNDEHNAARRRVSPDRRG
                     SQPDPGGPK"
BASE COUNT          286 a          696 c          606 g          258 t
ORIGIN      
        1 gcggcggctt cgacctggag gtgctggaag acgccaccgc gcccgagaac ctcctcaagg
       61 agcgcggccg cggcatcttc atcatgcggt cgatgatgga cgacgtgtcg ttcgacttca
      121 ccgacaaggg cacccgctgc cggctggtca agcgacgccg ggtgggtgag gccgacgtga
      181 gtggcgggcc cgcacccctt gacgacacgc tctgacgacc ggatcctggc cgtcgacccc
      241 ggaacgaagc gcatcggcct ggccgtggcc agcgtgacac aggggatggc ctcgcgcctc
      301 gccgtcctcg atgtccagcc gggcgcgagt ggcctcgagc gcgcggtccg cgccgtggcg
      361 cgtcacgccg ccgaggaaga ggccacccgc atcctggtgg ggctgccgct ccatctcgac
      421 ggacgggagg gaaaggaggc ccgcgccgcc cggcgcctcg gcctggcgct cgcggaggcc
      481 accggtcttc cggtcgactt tgccgacgag cgcctcacct ccgaggccgc catcgaggag
      541 gcgcgcaaga gcggctggac gccgaagagc aaaaagccga tcgacgacct ggccgcggcc
      601 ctcctcctgc agggatggct cgacgacgcc agcgcccggg cggcccgcca acgactcaac
      661 gaatcgacat gaccaccgcc gtcaagaacc tcctctggat cgccgcgctc ttcaccttcc
      721 tggccgccgg cgtggccagc ctcttcatgc cggccagcct cacccccggc gacccggcca
      781 tccgcatcac cctctctccg gggatgacca cgcggcagat cggcgccacg ctccggcgct
      841 cgggcctcat ccgctcgtcg gatgccttca tgctggtggc ccacctctcc ggccgggcca
      901 gccagctccg cgccggcacc tacgccatca gcgcctcgga agggatcggc cgcatcgtgg
      961 accgcctggc cgacggcgat gtcctccggg tggccgtcac gatcccggag ggattcaacg
     1021 cccgcgaaat cgccgcccgc gtggcgcccg tgctcggctg cccggtggag gagttcatgg
     1081 ccattgtcag cgactcggtc atggccgagt cgctcggggt gggggagggc accctcgagg
     1141 ggtatctctt tcccgacacc tacaacgtca tccccgggac cgacccggcg ggattcatcg
     1201 cccgcctcgt ggaacgcacc accaccctct tcgaggagcg ctaccgcgcc cgcgccgaca
     1261 gcctggggct ggctcgccac caggcgctca cgctggccag cctggtcgaa gccgaggcgg
     1321 cggtggacga ggaacgtccg cggatttccg ccgtcttctg gaaccggctg cgcgagggga
     1381 tgatgctcca gtccgacccg acggtggcct acgccctggg gcgtcgcccc gaccggatct
     1441 actacaagga cctcgaggtc caatccccct ataatacgta cctcgtgacc gggctcccgc
     1501 cggggccgat ctgttccccc ggcgaggcct cgatccgggc cgctctccac ccggtgccgg
     1561 gctcgaagga gctctacttc gtggccaccg gagacggcca ccatcttttt tccacgacca
     1621 acgacgaaca caatgccgcc cgccgacggg tcagcccgga ccggaggggg agccaacccg
     1681 atccaggagg accgaagtag catgtccact gccaaagggg cccgcaaagg gtccaccatg
     1741 cgggggaagg tggcccaccc ccgcgacgag gtgcgcctca cccggaagaa ctggatcctc
     1801 ttcggggcgg ccctcgcgga catcgtcctg gggtacatcc tcctcc
//
LOCUS       DASVOD010000642         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_405837, whole genome shotgun sequence.
ACCESSION   DASVOD010000642 DASVOD010000000
VERSION     DASVOD010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405837"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..>3322)
                     /locus_tag="VF720_13860"
     CDS             complement(173..>3322)
                     /locus_tag="VF720_13860"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7880495.1"
                     /translation="IGEEWLENPNGGAVANVGSTRFDFPGTGTDYQNEFYRILFQQPV
                     PSLGEAFAGSKLPFVSESGSDSEQRWTQFSQILLGDPSLRIWRYEPVAATVVHPPSIT
                     LGTEGVSITVTRGGSPVDSAIVCLSKPGDDYEVGRTNAAGQAVITFRPDHAGIVRLTV
                     THPTLRPWQADIPVTAPAAPHLFASASTFDDATPPAAGNGDGIFDLEETVRFFPTLAN
                     LGSVSAGSVTATLVSSDPNLVVVDGSATWPSIPAGNAFQALDPFVIRAAGTATDRYET
                     TATLNISAAGYSRSEPVVILVGAPLIERYRLQRTDVGSGNGNGILEAGETQTLTLTLR
                     NNGRGSVRNLVAKLRPLDAETTVLDSVASFGNVGADQVAVGDPFLFQWSVVAPSRKMR
                     LVVESLGKTLMSFDIDVYSPSTPQSPAAGGKATSISMTWTPNVETDMRGYVIYRGSAP
                     GGPFTRVNSYADMKMAYYNDENLPSLTRFYYRVAAIDQSGNEGNMSPVAAATTTLPAS
                     NGFPLLLGGSTSSSPAFSYFDGDTIPEIVTGAEELYVISGQGIEFVDGDNDTRTYGVY
                     SSSGLANFWSPPSSTDIDRDGVIDVAAAGWSNGLLFLYDGHLGTKPGFPVNIDVSQNG
                     NPNTWSAPVMVDVNNDGDMEIFINSAGHTYAFHDDGSELIDGDNNPATTGVFAVLGTP
                     ANYSTPRVCDLDNNGTMEVIIASRDKKLYVKNLNGTDYPGFPITYTGDMTSTPAIGDL
                     NNDGLKEIVFGCSDNKLYAFNINKVAPPGWPKGVNFNQDLDSSPALADMNGDGYLDVA
                     ICAGNGTVGIFQGQTGAIFPGFPVTLTSASGANIQIRSSPSIGNVDSSPDLEMVFGGQ
                     DGNLYALKPNGTMAIGFPIKTENVIEGGPLLWDLDGDGLTEVCVQSYDQRMYVWDSPA
                     PFTPASCPWPMFGHDSRRTGVMGNPIFIVTGTPEGEGSPRVPLELAQNSPNPFQPMTT
                     IRFARAPGSGSVAATLSVFDPSGRLVRRLFEGDVAEGTHEVRWDGRDDSGSPLPSGVY
                     FYKLTAGSHSVTRKMTLTR"
BASE COUNT          530 a         1047 c         1154 g          591 t
ORIGIN      
        1 gtccagggcc gggttccagc gacggcgtcg tgttcagcgg atttccgtta accagtccgt
       61 tgaggttttc cccagtcccg ttggttgtgc ggggtctccg tcaggcgacg gtgctcgaac
      121 cccgtgaggc taatggagcc cctgcaaggt gtcaattatc ggcaccgccg tgctagcgcg
      181 tgagcgtcat cttgcgcgtg accgaatgcg atcccgccgt cagtttgtag aaatagactc
      241 ccgaagggag cggcgagccc gagtcgtcgc gaccatccca ccggacttcg tgcgtcccct
      301 cggccacatc cccctcgaac agccggcgca ccaggcggcc ggagggatcg aacacggaaa
      361 gcgtggcggc cacggaaccc gaccccgggg cccgggcaaa gcggatcgtg gtcatcggct
      421 ggaacgggtt gggcgaattc tgggccagct ccagcggcac gcggggcgag ccctcccctt
      481 ccggcgtgcc ggtcacgatg aagatcggat tgcccatgac cccggtccgc cggctgtcgt
      541 gcccgaacat cggccatgga caggacgccg gggtgaaagg cgccggggag tcccacacgt
      601 acatccgctg gtcatagctc tgcacgcaca cttcggtcag gccgtcgccg tccaggtccc
      661 agaggagcgg gcccccttcg atcacgttct cggtcttgat cgggaagccg atggccatcg
      721 tgccgttcgg cttgagcgcg tacaggttcc cgtcctgccc gccgaacacc atctcgaggt
      781 cgggcgatga gtcgacgttg ccgatcgacg ggctggagcg gatctggatg ttcgcgccgg
      841 acgcgctggt cagcgtgacc gggaagcccg ggaagatggc gccggtctgt ccctggaaga
      901 tgccgaccgt gccgttgccg gcgcagatcg ccacgtcgag gtacccgtca ccgttcatgt
      961 cggccagggc cgggctcgaa tcgaggtcct ggttgaagtt gacccccttc ggccatccgg
     1021 gcggagccac cttgttgatg ttgaaggcat acagcttgtt gtcgctgcag ccgaagacga
     1081 tctccttgag gccgtcgttg ttcaggtccc cgatggccgg cgtggaggtc atgtcgccgg
     1141 tgtaggtgat cgggaaaccc gggtagtcgg ttccgttcag gttcttcacg tacagcttct
     1201 tgtcgcggct ggcgatgatg acttccatcg tgccgttgtt gtccaggtcg cacacccgcg
     1261 gcgtggagta gttggccggc gtgccgagga ccgcgaagac gccggtcgtg gccgggttgt
     1321 tgtcgccgtc gatgagctcg ctgccgtcgt cgtggaatgc gtacgtgtga cccgccgagt
     1381 tgatgaagat ttccatgtcg ccgtcgttgt tgacgtcgac catcaccggg gcactccagg
     1441 tgttcgggtt gccgttctgg ctcacgtcga tgttcaccgg gaagccgggc ttggtcccca
     1501 ggtggccgtc gtaaaggaag aggagcccgt tgctccaccc cgccgcggcc acgtcgatga
     1561 cgccgtcgcg gtcgatgtcc gtcgacgacg gcgggctcca gaaattggcc agccccgagc
     1621 tcgaatacac gccgtaggtg cgcgtgtcgt tgtcgccgtc gacgaactcg atcccctgcc
     1681 cgctgatgac gtagagctcc tcggcgccgg tcacgatttc gggaatcgtg tcgccgtcga
     1741 aatacgaaaa ggctggcgag gaggaagtgg aaccgccgag gaggagcggg aatccgttcg
     1801 aggcgggcag cgtggtggtg gccgccgcca cgggcgacat gttcccttcg ttccccgact
     1861 ggtcgatggc cgccacgcgg taatagaaac gggtgagcga cggcaggttc tcgtcgttgt
     1921 aataggccat tttcatgtcg gcgtaggagt tcacccgcgt gaagggccca ccgggggcgg
     1981 agccccggta gatcacgtag ccgcgcatgt ccgtttcgac attgggcgtc caggtcatgg
     2041 agatcgaggt ggccttgccg ccggcggccg gcgattgtgg tgtcgagggt gagtagacat
     2101 caatgtcgaa cgacatcaac gtcttgccca ggctctcgac caccaggcgc atcttccgcg
     2161 agggggccac cacgctccac tggaacagga acgggtcgcc gacggccacc tggtccgcgc
     2221 cgacattgcc gaagctggca accgagtcga ggaccgtcgt ctcggcgtcg agcggccgca
     2281 gcttggccac caggttgcgc acgcttccgc ggccgttgtt ccgcaacgtg agcgtgagcg
     2341 tctgcgtttc ccccgcttcg aggatgccgt tgccgttgcc gctcccgaca tccgtgcgct
     2401 ggaggcgata ccgctcgatc agcggcgccc ccaccaggat gacgaccggc tccgagcgcg
     2461 aatacccggc ggcggatatg ttgagggtcg ccgtcgtttc gtaacgatcg gtggccgtgc
     2521 cggccgcccg gatcacgaac gggtcgaggg cctgaaaggc gtttccggcg ggaatggacg
     2581 gccaggtcgc cgagccgtcc acgacgacca ggttcgggtc gctcgagacg agcgtggccg
     2641 tgacgctgcc tgccgacacg ctccccaggt tggccagcgt cgggaagaag cggaccgtct
     2701 cctcgaggtc gaagatcccg tcaccgtttc cggcggcagg gggggtcgcg tcatcaaagg
     2761 tggaggccga ggcgaagaga tgcggggcgg ccggagccgt caccgggatg tcggcctgcc
     2821 atgggcggag cgtcggatgc gtgacggtca accggacgat ccccgcgtgg tcgggccgga
     2881 aggtgatcac ggcctggccc gcggcgttcg tccttcccac ttcatagtca tcccccggct
     2941 tgctgaggca gacgatcgcg gaatccacgg gcgagccacc ccgtgtcacc gtgatcgata
     3001 cgccctccgt gccgagtgtg atcgacgggg gatgaacgac cgtggccgcc accggctcgt
     3061 agcgccagat gcggagggac ggatcgccga ggaggatctg gctgaactgc gtccagcgct
     3121 gctcggagtc cgagcccgac tcggagacga acggaagctt cgacccggca aaggcctcgc
     3181 cgaggctggg gaccggctgc tggaagagta tccggtagaa ctcattctgg tagtcggtgc
     3241 ccgtcccggg gaaatcgaac cgggtcgagc cgacgttggc caccgcgccg ccgttggggt
     3301 tctcgagcca ctcctcgccg at
//
LOCUS       DASVOD010000643         4439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_198702, whole genome shotgun sequence.
ACCESSION   DASVOD010000643 DASVOD010000000
VERSION     DASVOD010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198702"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VF720_13865"
     CDS             complement(<1..1087)
                     /locus_tag="VF720_13865"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX7880496.1"
                     /translation="MIHPEPVSILLVDDQPSRLLSYEAILEGLGQNLVRAGSGQEALH
                     LLMKQEFAVILLDVNMPTMDGFETANLIHQHPRYEKTPIIFVTAVHMTDLDRLKGYEL
                     GAVDYVYIPVVPEILRSKVAVLVELYRNRRELRSINEDLRRANDELERANDLLRAEKT
                     RELQELNYTLEEANVRLEAANRTLSESASRKDEFLALLAHELRNPLNPIRNVVEVLRM
                     DSSPPQVEWGRDLIDKQVRHLTRLIDDLLDINRITMNRLVLVRERVNLREIVMAALEA
                     SVDGRSSHPLSTSFPETPIELHGDSVRLTQIFMNLLNNALKFTPPGNAIHLTVAPTDA
                     EDPPMVEVRIRDEGVGIPAEQIGQLFDL"
     gene            complement(1098..>4439)
                     /locus_tag="VF720_13870"
     CDS             complement(1098..>4439)
                     /locus_tag="VF720_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7880497.1"
                     /translation="ILQLKETVNTMVDQLNAFGAEVTRVAREVGTEGKLGGQAEVKGV
                     AGTWKDLTDNVNVMANNLTNQVRGIVKVVTAVANGDLQQKLMVESKGEVAALADTINS
                     MTGTLATFADQVTTVAREVGVEGRLGGQANVPGAAGTWKDLTANVNLLAANLTTQVRA
                     IAEVATAVTKGDLTRTVQVDARGEVSELKENINAMIGNLRGTTERNREQDWLKTNLAR
                     FTGMLQGQRELDTVGRTLLTELVPLVDAHQGLIYQMETEANPQLRLLASYGNSQGGYP
                     ERIPLGEGFIGQCAADKRRILVSEVPPDAVPVGSALFMALPRSIVVFPVNFEGQLKAV
                     IGLTSLNSFSPLQLGFLEQLTSNIGIVLNSIEATMQTEGLLKQSQQLTNELQTQQKEL
                     QATNEQLGQKAQQLAEQNAEVERKNQEIEQARRDLEEKANELALTSKYKSEFLANMSH
                     ELRTPLNSILILGQQLMENIDGNLSSRQVEFAKTIHGAGTDLLNLISDILDLSKIESG
                     TVTVECEDLTFAGLRESIERGFRHEAENRRLSLLIDFELGALTSMTTDQKRLLQVLKN
                     LLSNAFKFTEQGGVRLKVAVAAGGWNPEHPVLSAADTVIEFAVHDTGVGIPIEKQKII
                     FEAFQQADAGTARKYGGTGLGLAISRELASLLGGEIRLHSVPGKGSTFTLYLPLQYSG
                     SARPALTSGASDRPASPFQPIALPVPVIEAVQDDRSNIIKGEPVLLIVEDDARYAGIL
                     LDLARERGFKGLVAGRGSDALQLARKHQPTAISLDIFLPDMLGWTVLSQLKQDPALRH
                     IPVQIVTVEEERQHGLERGAFSFMNKPLTTDTIEEAFDRIKNFAGRSKRRLLVVEDNP
                     AEQMSIVELIAHDDVEIGTASTGAEALALLMENSWDCLVLDLKLPDLSGFDLLARMQE
                     MPRLRDLPTIVFTGKELSADEEKQIRKMAKSIVLKGVESPERLLDETALFLHRVVTDL
                     PESMQRMLDRLHQSDEVLNGRRVLIVDDDVRNIFALSSLLERHRMSALTAMNGQEAIK
                     IIEATPDLSLVLMDIMMPEMDGYETIRRIREQPAFRTLPIIALTAKAMKGDRDKCLQA
                     GASDYIAKPVNTEQLLSLLRIWLHR"
BASE COUNT          660 a         1400 c         1510 g          869 t
ORIGIN      
        1 agaggtcgaa gagctggccg atctgctcgg ccgggattcc gacgccctcg tcgcggatcc
       61 gcacctccac catcggcgga tcctcggcgt cggtcggggc cacggtcagg tggatggcgt
      121 tgccgggcgg cgtgaacttg agggcattgt tcagcaggtt catgaagatc tgtgtcaacc
      181 ggacggaatc gccgtgcagc tcgatgggcg tttcagggaa cgaagtgctc aacgggtggg
      241 aggatcggcc atcgaccgag gcctcgaggg ccgccatcac gatctcccgg aggttcaccc
      301 gctcccgcac caggacgagc cggttcatcg tgatgcggtt gatgtcgagc aggtcatcga
      361 tgaggcgggt gaggtggcgc acctgcttgt cgatgagatc gcgaccccac tccacctggg
      421 ggggggagct gtccatgcgg agcacctcga ccacgttccg gatggggttc aacggattgc
      481 gcagctcgtg ggcgagcagt gccaggaact cgtccttccg gctggccgac tcggagaggg
      541 tgcgattggc ggcctcgagc cgcacgtttg cctcctccag cgtgtagttc agctcctgca
      601 gctcgcgcgt tttctcggcc ctgaggaggt cgttggcacg ttcgagctcg tcattcgccc
      661 ggcgaaggtc ctcgttgatg cttcgcagct cccggcggtt ccgatagagc tccaccagca
      721 ccgccacctt gctgcgcagg atctccggca cgacggggat gtaaacgtaa tcgaccgcac
      781 cgagctcgta ccccttgagc cggtcgagat ccgtcatgtg caccgcggtg acgaagatga
      841 tcggcgtctt ctcgtagcgg gggtgctggt gaatcaggtt ggccgtctcg aagccatcca
      901 tcgtcggcat gttgacgtcg agtaggatga ccgcgaactc ctgcttcatg agcaggtgaa
      961 gcgcttcctg tcccgagccc gcccgcacca ggttctggcc caggccctcg aggatggcct
     1021 cgtagctcag caggcgcgac ggctggtcgt cgaccaggag gatgctgacg ggttccgggt
     1081 ggatcatcgt catcccccta ccggtgcaac cagatgcgaa gaagcgacag gagctgttcg
     1141 gtgttcacgg gcttggcgat gtagtccgac gcgcctgcct gcaggcactt gtcccggtct
     1201 cccttcatgg ccttggcggt gagggcgatg atggggagcg tccggaaggc gggctgctcg
     1261 cggatgcgcc ggatcgtctc gtagccgtcc atctcgggca tcatgatgtc catcaggacc
     1321 agtgacaggt cgggcgtggc ctcgatgatc ttgatcgcct cctgcccgtt catggcggtc
     1381 agcgcggaca tccggtgacg ctcgagcaga ctgctgagcg cgaagatgtt gcggacgtcg
     1441 tcgtccacga tcaggacgcg ccggccgttc agcacttcgt ccgactggtg caggcgatcg
     1501 agcatccgct gcatcgattc cggcaggtcg gtgacgacgc ggtgcaggaa gagagccgtc
     1561 tcgtcgagca ggcgttcggg cgattcgacc cccttgagca cgatgctctt ggccatcttc
     1621 cggatctgct tctcctcgtc ggccgagagc tccttgccgg tgaagacgat cgtcggcagg
     1681 tccctgagcc gaggcatctc ctgcatgcgg gccaggaggt cgaacccgga cagatcgggg
     1741 agcttgaggt cgagcaccag gcagtcccat gagttctcca tcagcagggc cagcgcttcg
     1801 gcgcccgtgg aggccgtccc gatctccacg tcgtcgtggg cgatcagctc gacgatgctc
     1861 atctgttcgg ccgggttgtc ctccacgacc aggagccggc gcttgctccg cccggcgaag
     1921 ttcttgatgc ggtcgaatgc ttcctcgatg gtatcggtgg tgagcggctt gttcatgaac
     1981 gagaaggctc cccgctcgag gccatgctgc cgctcctcct ccaccgtgac gatctggacc
     2041 gggatatggc ggagggcggg gtcctgcttg agctgcgaga gcaccgtcca gccgagcatg
     2101 tccggcagga agatgtcgag cgagatggcc gtgggttggt gcttgcgggc cagctgaagg
     2161 gcatcgctcc cccgccccgc caccagtccc ttgaagcccc gttctcgcgc caggtcgagc
     2221 aggatgccgg cgtagcgggc gtcgtcctcc acgatgagga ggaccggctc tcccttgatg
     2281 atattgctcc ggtcgtcctg caccgcctcg atgaccggca ccggcagcgc gatcggctgg
     2341 aacgggctgg ccggcctatc ggaggcgccc gaggtcagag ccggacgggc cgatccgctg
     2401 tactggagcg gcaggtacag ggtgaacgta ctccccttcc ccggcacgct gtgcaggcgg
     2461 atttcgcctc ccaggaggga ggccagctcg cggctgatgg cgaggccgag gccggtgccg
     2521 ccgtacttgc gcgccgtgcc ggcatccgcc tgctggaacg cctcgaagat gatcttctgc
     2581 ttctcgatgg gaattcccac gcccgtgtcg tggaccgcga actcgatgac cgtgtcggcg
     2641 gcggagagga ccgggtgttc gggattccag ccgccggctg ccaccgccac cttgagcctc
     2701 accccaccct gctcggtgaa cttgaatgcg ttggagagga ggttcttcag cacctgaagc
     2761 aggcgcttct ggtcggtggt catcgaggtc agcgccccca gctcgaagtc gatcaggagc
     2821 gacaaccgcc ggttctcggc ctcatggcgg aagccgcgct cgatcgactc gcgcaggccg
     2881 gcgaaggtga ggtcctcgca ctccaccgtg accgtccccg actcgatctt ggagaggtcg
     2941 aggatgtcgg agatgagatt caacaggtcg gtgccggcgc cgtggatcgt cttggcgaac
     3001 tccacctggc ggctcgacag gttcccgtcg atgttctcca tgagctgctg gccgaggatc
     3061 aggatgctgt tgagcggcgt ccggagctcg tgcgacatgt tggccaggaa ctccgacttg
     3121 tacttggagg tgagggccag ctcgttggcc ttttcctcga ggtcgcgccg ggcctgctcg
     3181 atctcctggt tcttccgttc cacctcggcg ttctgttcgg ccagctgctg cgccttctgg
     3241 ccgagctgct cgttggtggc ctggagctcc ttctgctggg tctgcagttc gttggtgagc
     3301 tgctggctct gcttgaggag cccctcggtc tgcatggtgg cctcgatcga gttgagcacg
     3361 atgccgatgt tcgaggtgag ctgctcgagg aagccgagct gcaggggaga gaacgagttc
     3421 agcgaagtga ggccgatgac ggccttgagc tgcccctcga agttgaccgg aaagacgacg
     3481 atgctgcgcg gcagcgccat gaagagcgcc gagccgaccg gcacggcgtc gggcggcacc
     3541 tcgctcacga ggatgcgccg cttgtccgcc gcgcactggc cgatgaaccc ctcgcccagc
     3601 ggaatccgtt ccgggtatcc cccctgggaa tttccgtagc tggccaggag ccgtagctgc
     3661 ggattggcct cggtttccat ctggtagatg agcccctggt gggcgtcgac cagcggcacg
     3721 agctccgtga ggagcgtgcg acccacggtg tcgagctcgc gctgcccctg gagcatgccg
     3781 gtgaagcggg ccaggttggt cttgagccag tcctgctcgc ggttgcgttc ggtggtgccg
     3841 cgcaggttgc cgatcatggc attgatgttt tccttgagct ccgacacctc gccgcgggca
     3901 tccacctgca cggtgcgcgt gagatccccc ttggtgacgg cggtggccac ctcggcgatg
     3961 gcgcgcacct gcgtggtgag gttggccgcg agaaggttca cgttggcggt gaggtccttc
     4021 caggtgccgg cggcgcccgg cacgttggcc tggccgccga ggcgcccttc cacgcccact
     4081 tcacgggcca cggtggtcac ctggtcggcg aaggtagcca gcgttccggt catcgagttg
     4141 atcgtgtcgg cgagcgcggc cacttcgccc ttcgattcca ccatcagctt ctgctgcagg
     4201 tcgccattgg ccacggccgt caccaccttc acgatgccgc ggacctggtt ggtgagattg
     4261 ttggccatga cgttgacgtt gtcggtcagg tccttccagg tgccggccac ccccttcact
     4321 tcggcctggc cgccgagctt cccttcggtg cccacttcgc gggccacgcg ggtgacctcg
     4381 gcgccgaagg cgttcagctg gtcgaccatc gtgttcacgg tttctttcag ctgcaggat
//
LOCUS       DASVOD010000644         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_667026, whole genome shotgun sequence.
ACCESSION   DASVOD010000644 DASVOD010000000
VERSION     DASVOD010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667026"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..666)
                     /locus_tag="VF720_13875"
     CDS             complement(136..666)
                     /locus_tag="VF720_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007810426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880498.1"
                     /translation="MTHIPVDDGLRWLLWRQFGAAIDMLGNAITACPDDVWSDRTRRP
                     EYWYTAYHTLFWLDLYLHGPVEGFRPLPPFGLEELDPAGVLPDRVYTRSELMTYLEHG
                     RERCREAVAGLDAEGAARPLRFGWGHTTYLELLLYNMRHVQHHAAQLNLILRQSVDDA
                     PRWVGITKHPGPGIPA"
     gene            complement(663..1088)
                     /locus_tag="VF720_13880"
     CDS             complement(663..1088)
                     /locus_tag="VF720_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HEX7880499.1"
                     /translation="MPGIERTATGTWRGNLKGGNGTISATSGVLSGTPFTFATRFENA
                     RGTNPEELIAAGQAGCYSMALSHHLSEQGHVPDEVTTTATITLDGGVINRMHLKTVGK
                     VPGLDDATFKRMAREAEQKCPVANLLRGGLTITLDASLA"
     gene            complement(1103..1450)
                     /locus_tag="VF720_13885"
     CDS             complement(1103..1450)
                     /locus_tag="VF720_13885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880500.1"
                     /translation="MTELIARLVFVAAFAVAVGAYGRLRRRYPRREWQWWPIVLAALV
                     GGAAFYASQQIARPHLPGLLDLPAFLIMVVAAGTMFVVFEAAVVSKHAGRTRQAATRT
                     DADPEDSHQGHPT"
     gene            complement(1479..2006)
                     /locus_tag="VF720_13890"
     CDS             complement(1479..2006)
                     /locus_tag="VF720_13890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEX7880501.1"
                     /translation="MTGDTLVIRRAGRNDLDSLVALTVRAFHQAYDGKSPAPEIDAHI
                     ARHFTNDKLVAEITDPDTIMLLADSAEGLLGYALLRVGSATPLVPEPGPVELCRIYFD
                     AAASGRGHGSALMPACLEAAREKGGGVIWLSVWEENHAAIRFYERWGFRRVGTQPFDF
                     GGTWYEDPIMSRLLA"
     gene            complement(2003..>2354)
                     /locus_tag="VF720_13895"
     CDS             complement(2003..>2354)
                     /locus_tag="VF720_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880502.1"
                     /translation="CFVFDNRPMHSHDAADTTHHHEDEVRLISIGCPGSNPPGVRALE
                     SGGPEEAALLDAFDLYADTFVRPAQRESLLTTPYYYWYRLSDEQNRARIVLGTAKFLR
                     EQAAKQAAADSTGP"
BASE COUNT          378 a          776 c          799 g          401 t
ORIGIN      
        1 attgctcgtc tcccgccagg ttgagccggt tcacacggat tggacccgac acattcgatt
       61 tccagatgga ggttcggaca ctttcgccgt tccagctcac ctcacgcggc ccgccgacag
      121 acacggtgag gagtttcacg cggggattcc cgggccgggg tgtttcgtga tgccgaccca
      181 gcgcggggcg tcatccaccg actggcgcag gatgagattg agctgcgcgg cgtgatgctg
      241 cacgtgccgc atgttgtaga gaagcagttc cagatacgtg gtgtgtcccc aaccgaaccg
      301 gagcgggcgc gccgcgccct ccgcatccag tcccgccacg gcttcacggc agcgctcgcg
      361 gccatgctcg aggtatgtca tcagctcgga tctcgtgtac acccgatcgg gaagcacgcc
      421 ggccggatcg agctcctcga ggccgaacgg cggcaggggc cggaatccct ccaccggccc
      481 gtgcaggtag aggtcgagcc agaagagcgt gtgatacgcc gtgtaccagt attccggccg
      541 acgggtgcgg tcgctccaga catcgtccgg gcaggccgtg atggcgttgc ccagcatgtc
      601 gatggcggcg ccgaattgcc tccacagcag ccaccgcaat ccgtcgtcca ccgggatgtg
      661 cgtcatgcca gggaggcatc cagcgtgatc gtgagaccac cgcggaggag gttggccacg
      721 gggcacttct gttccgcctc ccgggccatg cgcttgaacg tggcgtcgtc gaggccgggc
      781 accttcccca ccgtcttcag gtgcatcctg tttatgacac ccccgtcgag cgtgatggtg
      841 gccgtggtgg tcacctcgtc gggcacgtgt ccctgctcgc tcagatgatg cgagagggcc
      901 atgctgtagc aaccggcctg gccggcggcg atcagttcct ccggattggt ccccctggcg
      961 ttctcgaacc gggtggcgaa ggtgaacggt gttccggaca ggacgccgct cgtggcgctg
     1021 atcgtcccgt tgccgccttt caggttgccg cgccaggttc cggtggcggt gcgttcaatt
     1081 ccaggcatgg tgactgtcct tcctaggttg gatggccctg gtgagaatct tcggggtcgg
     1141 cgtcggtcct cgtcgcggcc tgacgcgtcc gaccggcatg cttgctgacg accgcggctt
     1201 cgaaaaccac gaacatggta ccggcggcca ccaccatgat caggaaggca gggagatcga
     1261 gcaagcccgg cagatgcggc cgggcgatct gctgcgaggc atagaaggct gcgcccccca
     1321 ccagggccgc cagcacgatc ggccaccact gccattcgcg acgcgggtaa cgacgccgga
     1381 ggcggccgta ggcgcccacg gccaccgcga aggccgccac gaagaccagc cgggcgatca
     1441 gttccgtcac gtcaggaact ccgcgacaat tcggcgtgtc acgcgaggag ccgcgacatg
     1501 atcgggtctt cgtaccacgt gccgccgaag tcgaacggct gcgtgcccac ccggcggaat
     1561 ccccaccgtt cgtagaaccg gatggccgcg tggttctcct cccacacgct gagccagatc
     1621 acgccgccac ccttttcccg cgccgcctcg aggcaggccg gcatgagtgc cgatccgtgc
     1681 ccccggccgc tggcggcggc gtcgaagtag atgcggcaca gctccaccgg accgggctcc
     1741 ggaacgagcg gtgtggcaga accgacgcga agcagcgcgt acccgagcag cccttcggcc
     1801 gaatccgcca gcagcatgat ggtatccggg tcggtgatct cggcaacgag cttgtcgttc
     1861 gtgaagtgcc gggcgatgtg cgcgtcgatc tccggcgccg ggctcttgcc gtcataggcc
     1921 tggtggaagg cgcggaccgt gagggcaacc agcgagtcga ggtcgtttct cccggcccga
     1981 cggatgacca gggtatcgcc cgtcatggac cggtcgagtc ggccgccgcc tgcttcgcgg
     2041 cctgctcgcg caggaatttc gcggtcccga ggacgattcg ggcccggttc tgctcgtcgc
     2101 tcaaccggta ccagtagtag tagggcgtcg tgagcaggct ttcgcgctgg gcggggcgca
     2161 cgaacgtgtc ggcataaagg tcgaacgcgt cgaggagcgc cgcttcctcc ggcccgcccg
     2221 attcgagcgc acgcacgccg ggcgggttcg aaccggggca accgatgctg atcaggcgca
     2281 cctcatcttc gtggtggtgc gtggtgtcgg cggcatcgtg cgaatgcatc ggtcggttgt
     2341 cgaacacgaa acag
//
LOCUS       DASVOD010000645         3323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_45234, whole genome shotgun sequence.
ACCESSION   DASVOD010000645 DASVOD010000000
VERSION     DASVOD010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45234"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            564..2090
                     /gene="istA"
                     /locus_tag="VF720_13900"
     CDS             564..2090
                     /gene="istA"
                     /locus_tag="VF720_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007195731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX7880503.1"
                     /translation="MKHFRDILRLRHELGLTQREISRSLGIGLGTVSEYLRRAEQAGL
                     SWPEAAALDEPEIEARLFPRDPDQDPPRTPPDLPAIHRELKRVGVTLQLLWVEYIQAN
                     PGGYRYSQFCELYRRFAKKLGPVMRQVHKAGEKTFVDFSGKKPEIVDPMTGVVTSVDL
                     FVGAMGASHYFYAEATPDQTLGSWIGAHVRMVEWFGAAPQVFVPDNLKSGITDACRYE
                     PGVNRSYEEFARYYGAVVIPARVVRPRDKAKVENAVLLAQRWILAALRNRTFFSLCDL
                     NDAIRQLLPILNARLLQKLGVSRRELFDRVDRPAMNPLPPSRYELATWKPVTVNIDYH
                     VEVDRNFYSVPHALLREKVEARVTQAVVEIIFRSSRVTSHRRLTGRGLYATKPEHMPA
                     SHRAHAEWTPSRLVSWASSNGVATGQLVAGILESRPHPEQGYRACLGIMRLGRKHGAE
                     RLEAACERAVRLAAPSYRTVYNILAAGADRLPLNEEVTAPAPIHENIRGAAYYEEDPC
                     "
     gene            2084..2839
                     /gene="istB"
                     /locus_tag="VF720_13905"
     CDS             2084..2839
                     /gene="istB"
                     /locus_tag="VF720_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX7880504.1"
                     /translation="MLNQATIDKMQAMKMSAMAEAFHRQIESGQWADLSFEERLGMLV
                     DTEWTSREQKKLDRRLKTARLRYPASLEDVDYTANRGLDRQAVLALETCGWIKDHHNL
                     ILTGTTGTGKSYIACAFAERACRSGYGAAYYRTPRLLQELHVARGDGSYNRLMARLAR
                     VDLLALDDWLINPLKDQERRDLLEVIEDRNEKGSTLVTTQLPVKAWHEAIGDPTLADA
                     ICDRLIHRAHRIDLKGPSMRQAKSPVKKGAKAA"
     gene            3118..3323
                     /locus_tag="VF720_13910"
                     /pseudo
     CDS             3118..3323
                     /locus_tag="VF720_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345585.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          641 a         1118 c         1040 g          524 t
ORIGIN      
        1 ctatgaagag agggtctgcc gaaaaattcc cctgatagac cgcctgccac gccacgcacc
       61 caccttccca gtcaccccca gcattgccga acacatcgca gcagccgaac gtggggctcg
      121 ccgactgaca ggagacagct ccctggccga cattgaacgc cagaattgtt tgcgaaatag
      181 ccggattgca cgccttcgcg tgaagcgctc cacctccccc cccgatcgaa ctcgtggcct
      241 gattgcctac catcgtgcat gaggtaatga tagaagtctg atcctggcac gagacggctc
      301 cgccgtatgg agctgaattg ccggccagga tgcactccga catgctccct ccggagagct
      361 gcgtattccg cccatcctga acactgattc cggaggaaac tgaacaccga ttccgggcaa
      421 ggtgaacatc gattccggca tggtgaacag ccattccggg atggtgaaca ccgattccgg
      481 gcatcgtgaa cacgcgtcgg tagcgatctg gtccatcgtg ggccctcaaa ggagggctcc
      541 ggatggctgc ccagaggcta cggatgaagc actttcggga tatcttacgg ctccgccatg
      601 agttaggtct gacccagcgg gagatctcgc gttccctggg catcggcctc ggtacggtct
      661 cggagtacct gaggcgggcc gagcaggccg gactttcgtg gccggaggcg gccgccctcg
      721 acgagcccga gatcgaggcc cgacttttcc cccgcgatcc cgaccaggat ccgccgcgca
      781 cgccgccgga cctgccggcg atccaccggg agctgaagcg tgtgggcgtc acgctgcagt
      841 tgctgtgggt cgagtacatc caggccaacc cgggcggcta ccgctacagc cagttctgcg
      901 agctataccg gcgcttcgcc aagaagttgg gacccgtgat gcgccaggtc cacaaggccg
      961 gcgaaaagac cttcgtcgac ttctccggca agaagccgga gatcgtggat ccgatgaccg
     1021 gcgtggtcac gtccgtggat ctgttcgtcg gtgccatggg cgccagccac tacttctacg
     1081 ccgaggcaac gcccgaccag acactcggct cctggatcgg tgcccacgtc cggatggttg
     1141 agtggttcgg cgccgcgccg caggtcttcg ttccggacaa cctgaagagc ggcatcacgg
     1201 acgcctgtcg ctacgagccg ggtgtgaacc ggagctatga ggagttcgcc cgctactacg
     1261 gagcggtcgt gatccccgcc cgggtagtcc ggccgaggga caaagccaag gtcgagaacg
     1321 ccgtgctcct ggcccagcga tggatcctcg ccgcactgcg caaccgcacg ttcttcagcc
     1381 tgtgcgacct gaacgacgcc atccggcagc tcctgccgat cctgaacgcc cgcctgctcc
     1441 aaaagctcgg cgtcagccgc cgggagttgt tcgaccgcgt cgatcggccg gcgatgaacc
     1501 cactgccacc gtcccgttac gaactggcca cctggaagcc ggtcaccgtc aacatcgact
     1561 accacgtcga ggttgatcgc aacttctaca gcgtgccgca cgcgctcctc cgcgagaagg
     1621 tcgaggcgcg tgttacccag gcggtcgtcg agatcatctt ccgatccagc cgcgtcacct
     1681 cccatcggcg gctgacgggc cgcggactgt acgcgacgaa gccggaacac atgccggcct
     1741 cccaccgcgc ccacgccgag tggacgccat cgcgcctcgt cagctgggcc agctccaacg
     1801 gcgtagcgac cggccagctc gtcgccggca tcctggagag ccgcccgcac ccggagcagg
     1861 gatatcgcgc ctgcctcggc atcatgcgcc tcgggcgcaa gcacggggcc gagcggctgg
     1921 aggctgcgtg tgagcgtgcc gtccggcttg ccgcgcctag ttatcgcacc gtctacaaca
     1981 tcctcgcggc cggagcagat cgtctgcccc tgaacgagga agtcaccgcg ccggcgccga
     2041 tccacgagaa catccgcggc gccgcctact acgaggagga tccgtgctga accaggccac
     2101 catcgacaag atgcaggcca tgaagatgtc cgcgatggcc gaggcgttcc atcgccagat
     2161 cgagtccggt cagtgggctg acctgagctt cgaggagcga ctcgggatgc tggtcgacac
     2221 cgagtggacc agccgggagc agaagaagct cgatcggcgt ctgaagacgg ccaggctccg
     2281 atatccggca tcgctcgagg acgtcgacta caccgccaac cgcggcctcg accgccaggc
     2341 ggtcctcgcc ctcgagacgt gcggctggat caaggaccac cacaacctga tcctgaccgg
     2401 cacgaccggc accggcaaga gctacatcgc ctgcgccttc gcggagcggg cctgccgttc
     2461 cggctacggt gccgcttact accgaacgcc ccggctgctc caggagctgc acgtggcccg
     2521 cggcgacggt tcctacaacc gactcatggc ccggctcgcc cgcgtggatc tcctcgcact
     2581 cgacgactgg ctcatcaacc cgctcaagga ccaggaacgc cgcgacctgc tcgaagtcat
     2641 cgaggaccgc aacgagaagg gctccacgct cgtgacgaca cagctgccgg tcaaagcctg
     2701 gcacgaagcc atcggcgatc cgacactcgc ggatgccatc tgcgaccggc tgatccatcg
     2761 ggcccatcgg atcgacctca aggggccgtc catgcggcag gcgaagagtc cggtcaagaa
     2821 gggcgcgaag gcggcgtgaa gcaaacgaca cgcgaaatcg agtaacctgt cgttctggac
     2881 catcggggag gaaagggcga accagctcgc gtcgctccgg cgcgcgttca cgatcaccgg
     2941 aatcggtgtt cggcttcccg gactacgtgt tcagcttggg cggactacgc aaagaggcgg
     3001 cacgccaacg cgcgtgcgtg cggttcgaga cggcacccgg cctcgagggc cagttcgact
     3061 ggaagggacc gatccgcggg ctcctcgaga acgctccgga cgttccgaga gctcgaggag
     3121 ttcatcgaaa agtggggcgc tcggtacccg aagatcggcg aatcgtggcg cggcaagtgg
     3181 gacgtgctgt ccgggttctt cagttatcca cccgaaatcc gaaaggtgat ctacacgacc
     3241 aatgcggtag aatcaatcaa cgcttcgctg aggaaagtga ctcggaagcg gggagccttc
     3301 ccgaacccgg agtcggtgag gaa
//
LOCUS       DASVOD010000646         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_199002, whole genome shotgun sequence.
ACCESSION   DASVOD010000646 DASVOD010000000
VERSION     DASVOD010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199002"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VF720_13915"
     CDS             <1..1025
                     /locus_tag="VF720_13915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880505.1"
                     /translation="VAGHAGSPPLVEARRRGLQVLDAFDFRQGRLDIPWTLGRFRRYL
                     GKSHFDVVNAHRADDHVVAALALERNGPPLVRTRGDVRPPRRHLLNRWLYERLTSTHV
                     MAAEFMRTPFYSGFSIPAEKLVTIRPGLDVDAWREGAGNRWNTRDGLAIPRGARLVGV
                     IGRLAEAKGHLVALEAFARLARRFPEARLVIAGAPEELTPALLNERAQHLGIDRVVKL
                     LPRQDDIRPLMAALDVGLIASTASEAICRVALEYQALGIPVVGSDLNSIPEMVVHGKT
                     GLIVPPGDPAAVALSLERLFTEPSLADRLGAAGPDHVRKLYGLKTMVDRTEALYRKLI
                     RENAGD"
     gene            complement(1050..1892)
                     /gene="ybgF"
                     /locus_tag="VF720_13920"
     CDS             complement(1050..1892)
                     /gene="ybgF"
                     /locus_tag="VF720_13920"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HEX7880506.1"
                     /translation="MTLSLRAGRLASLAAGLCASILLVGCSPRQYFQQPAFVEESLDT
                     LKIMRAEQSAMALKVSSLESQLRDQTARSAEREANLNAELTMLNESLERLSGQLENLG
                     EQVQRRARPAPAPAWQPTPPPLPSGEPAPATNGAVPDSVIGAAGAPPSTWTPPPGNPG
                     EIYDRAYRDVTRGNYELASQGFRDFLDRFPDDDLSDNAQYWLGECFYVQDQVSEAARE
                     FEKVVNEHPRGDKVPAAYLKLGYCSLRKNDNAGARRWFDELIKKYPSTEEARSARNKL
                     ASIE"
     gene            complement(1892..>2387)
                     /gene="pal"
                     /locus_tag="VF720_13925"
     CDS             complement(1892..>2387)
                     /gene="pal"
                     /locus_tag="VF720_13925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEX7880507.1"
                     /translation="IAAVALVATGCSKKTTPPDVTQTTPAPDAGNNGGNNDANATPVD
                     KVPGDDADNANLKDIYFDYDDHTLSADARSTLSGNASYLREMGAIRVNIEGHCDERGT
                     VEYNLALGQRRADATRSYLVDLGIESSRLSTISYGEERPFEMGHDESAWHQNRRAHFR
                     VVNP"
BASE COUNT          361 a          805 c          821 g          400 t
ORIGIN      
        1 tcgtggctgg ccacgccggg agcccgccgc tcgtcgaagc ccgacggcga ggcctgcagg
       61 tgctcgacgc cttcgacttc cgccagggac gcctggacat cccctggaca ttgggccgct
      121 tccgtcgcta cctcgggaag agccacttcg acgtggtcaa cgcgcaccgg gccgacgacc
      181 acgtggtggc ggccctcgcg ctcgaacgga acgggccgcc gctcgtccgg acgcgcggcg
      241 acgtgcgccc gccacgccgt catctcctga accgctggct ctacgagcgc ctgaccagca
      301 cgcatgtgat ggcggccgaa ttcatgcgga cacccttcta ctccggattc tccatcccgg
      361 cggaaaagct ggtgacgatc cgaccagggc tcgacgtgga cgcctggcgg gaaggcgccg
      421 gcaatcgctg gaacacgcgc gacgggctcg ccatcccccg tggggcccgg ctggtcggcg
      481 tgatcgggcg cctggccgaa gccaaggggc acctcgtggc gctcgaagcc ttcgcccgcc
      541 tcgccaggcg ctttccggag gcgcgcctgg tcatcgcggg cgccccggaa gaactgactc
      601 ccgcgctcct caacgagcgc gcccagcacc tcggcatcga ccgcgtggtc aagctcctgc
      661 cgaggcagga cgacatccgt ccgctgatgg ccgccctcga cgtgggcctc attgcctcca
      721 ccgcctccga ggccatctgc cgggtggccc tcgaatacca ggcactcggc atcccggtgg
      781 tcggctccga tctcaattcg atcccggaga tggtcgtaca cggcaagacg ggactgatcg
      841 ttccccccgg cgatccggct gccgtggcgc tttcgctcga gcggctgttc accgagccat
      901 cgctggccga tcggctgggg gcggccgggc cggatcacgt gcggaagctg tacggcctta
      961 agaccatggt ggaccggacg gaggcgctct accgaaagct catccgggag aacgccggcg
     1021 actgacgcga gggcgcaaag ccggccggac tattcgatcg acgccagctt gttgcgggcg
     1081 gaccgggctt cctcggtgga gggatatttc ttgatcagct catcgaacca gcgccgcgct
     1141 cccgcgttgt cgttcttccg gagcgaacag tagccgagct tgagataggc ggccggcacc
     1201 ttgtcgccgc gcggatgctc gttgaccacc ttctcgaact cccgcgccgc ctcgctcacc
     1261 tggtcctgaa cgtaaaagca ctcgcccagc cagtactgcg cattgtcgga aaggtcgtcg
     1321 tcggggaatc tgtcgaggaa gtcgcggaag ccctgcgagg ccagctcgta gtttccacgc
     1381 gtcacgtcgc ggtaggcgcg atcgtagatt tcgcccggat tgccgggcgg gggtgtccac
     1441 gtggaaggcg gagcgcccgc cgccccgatg acgctgtcgg ggaccgcacc attcgtggca
     1501 ggagcgggct caccggacgg taacggcggc ggggtgggtt gccaggccgg tgcgggcgcc
     1561 gggcgggcgc gcctctgcac ctgttccccg aggttctcca gctggccgga gaggcgctcg
     1621 agcgactcgt tcagcatggt gagctcggcg ttgagattgg cctcccgttc cgccgaccgt
     1681 gccgtctggt ctcggagttg cgactcgagc gacgacacct tcaaggccat ggccgactgc
     1741 tcggccctca tgatcttgag cgtgtcgagc gactcttcga cgaacgccgg ctgctggaaa
     1801 tactgccgcg gcgaacagcc gacgaggagg atggaggcgc acaggccggc cgccagcgag
     1861 gcgagccggc ccgcgcgaag ggacaatgtc atcagggatt gaccacgcgg aagtgcgccc
     1921 ggcgattctg gtgccaggcc gattcatcgt gacccatctc gaatggacgc tcttcaccgt
     1981 aggagatcgt gctcagccgc gacgactcga tcccgaggtc gaccaggtag gagcgcgtgg
     2041 cgtcggcccg gcgctggcca agggccaggt tgtactccac cgtgccgcgc tcgtcgcagt
     2101 gaccttcgat gttgacgcgg atcgccccca tttcgcgcag gtacgaagca ttcccggaaa
     2161 gcgtggaacg cgcatcggcc gagagcgtgt ggtcgtcgta gtcgaagtag atgtccttga
     2221 gattggcgtt gtcggcgtcg tcccccggca ccttgtcgac gggcgtggca ttggcgtcgt
     2281 tgttgccgcc gttgttcccg gcatcgggcg ccggtgtcgt ctgggtgacg tcgggcggcg
     2341 ttgtcttctt ggagcaaccg gtggccacga gggccacggc cgcgatc
//
LOCUS       DASVOD010000647         2774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_460546, whole genome shotgun sequence.
ACCESSION   DASVOD010000647 DASVOD010000000
VERSION     DASVOD010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460546"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VF720_13930"
     CDS             <1..795
                     /locus_tag="VF720_13930"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880508.1"
                     /translation="PWFLQVKEGGYVNRMGRVTAFSMFVNDSPGSTGGTLYTTNHQPL
                     PQPTIEGGLVPVTLWIPEESPVPVAFARFEAVAEAEGIRLVLELTEESDAVGARLWRG
                     LTPVFEDRLELTHSPIPIEGRRLDYTDRTVENGGDYSYWVEVVGRDGSSAMAGPVTAR
                     LAVPARPTLAQPVFPNPVRASASFRYTIGTDGAAGGMVPVSLTLHDVQGRVVRHLASA
                     DAQPAGDYEVSWDGRDDAGRRLSSGVYYYLFRGGAVTSTSKVHVVN"
     gene            complement(903..1412)
                     /locus_tag="VF720_13935"
     CDS             complement(903..1412)
                     /locus_tag="VF720_13935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880509.1"
                     /translation="MRKLVMTIAAVIAVATLATGTAEAVNMKGKWGPGVSFEETPLDV
                     KFGLSPKSAVFFGLGFSDTDIEGSDTDFGLGAGFEYALFGNDDFNLNFQPSIQYFTGG
                     PSGDLIRIPVALSAEVWMNDRISVGMSHGIAINMFSDDDDTEFDEGQTTIGTFGASVT
                     AFRFRFYVK"
     gene            complement(1528..2034)
                     /locus_tag="VF720_13940"
     CDS             complement(1528..2034)
                     /locus_tag="VF720_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880510.1"
                     /translation="MRKFLAIAALAAVLSTAGATLARADMGALDGDQKKGDWFIGFWD
                     LNAPVAVRYMLTDDWEFGAGIGFNKPDEGDTGFALAFMATRRLIHASRAHLGVRPTVF
                     IESNNPSDVFAIGADLVAEIEIASAVSLVASHGLEYISVDNGADDTSTIQTRLSTGSR
                     VGFWVELP"
     gene            complement(2444..>2774)
                     /locus_tag="VF720_13945"
     CDS             complement(2444..>2774)
                     /locus_tag="VF720_13945"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006836669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX7880511.1"
                     /translation="DPEVMGIGPVPAVRALLEKTGTTLADHDLVELNEAFAAQVLAVD
                     RELRFDPERLNVNGGSIALGHPIGASGARIVVTLLHEMRRRAVRRGLATLCVSGGLGI
                     ASTFVRD"
BASE COUNT          503 a          883 c          899 g          489 t
ORIGIN      
        1 ccctggttcc tgcaggtgaa ggagggtggc tacgtaaacc gcatgggtcg cgtgaccgct
       61 ttctcgatgt tcgtcaacga ctccccgggc tcgaccggcg gaacgctcta caccaccaac
      121 catcaaccgc tgccgcagcc gacgatcgag ggaggcctcg tgccggtgac gctctggatc
      181 cccgaggagt cgccggtgcc ggtggccttt gcccgattcg aggccgtggc cgaagcggag
      241 gggatccggc tggtgctgga gctgaccgag gagagtgacg ccgtcggggc gcgcctttgg
      301 cgtggcctca cgcccgtctt cgaggatcgc ctcgagctca cgcactcccc catcccgatc
      361 gagggccgcc ggctcgacta caccgaccgc acggtcgaaa acggtggcga ctactcgtac
      421 tgggtggaag tcgtcggccg cgacggctcc tcggcgatgg cgggccctgt caccgcccgg
      481 ctggccgtcc cggcgaggcc cacgctggcc caaccggtct tcccgaaccc ggtgcgcgcc
      541 tcggcctcgt tccgctatac gatcggaaca gatggagccg ccggaggcat ggtgccggtg
      601 tcactcacac tgcacgacgt gcagggacgc gtcgtgcgtc acctggcctc ggccgacgcg
      661 cagccggccg gcgactacga ggtttcgtgg gacggccgcg atgacgccgg gcgccgcctc
      721 tcctcggggg tgtactacta cctcttccgg gggggcgcgg tgacgagcac atcgaaggtc
      781 catgtcgtca actagatctt gagtagtgtc gaccgcgtca tggacgtctc cctgaaaaca
      841 caagtgggag cttcccgttc ggggagctcc cacttgctga caagactgtt gaagtgtgcc
      901 ggttacttca cgtagaaccg gaagcggaac gccgtgaccg aggcgccgaa ggtgccgatg
      961 gttgtctggc cttcgtcgaa ctcggtgtcg tcgtcatcgc tgaacatgtt gatcgcgatg
     1021 ccgtgcgaca tgccgaccga aatccggtca ttcatccaga cctcggcgga aagggccacc
     1081 ggaatgcgga tgaggtcgcc gctgggcccg ccggtgaaat actggatgct cggctggaag
     1141 ttgaggttga agtcgtcgtt tccgaagagg gcgtactcga acccggcgcc gaggccgaag
     1201 tccgtatcgc ttccctcgat gtccgtgtcg gaaaacccga ggccgaagaa gacggcactc
     1261 ttcggcgaga ggccgaactt cacgtccagc ggcgtctcct cgaaggagac tcccggaccc
     1321 cacttgccct tcatattgac ggcttccgcc gttcccgtgg ccagggtcgc aacggcgatc
     1381 accgccgcga tggtcattac gagcttgcgc attcttcctc caaacttgaa tcaccttcaa
     1441 ggtgatgtgg attcagtatt ggtagccgga acatcgaaac tggaaagctc tctcggcgcc
     1501 gtgccccgaa gggggcccgc ggtcgtgcta cgggagttct acccagaatc cgaccctgct
     1561 tccggtgctc aagcgcgtct ggatcgtgga cgtgtcgtcg gcgccgttat cgactgagat
     1621 gtactcgagg ccgtgcgagg ccaccaggct cactgccgag gcgatctcga tttcggcgac
     1681 aaggtccgcg ccgatggcga agacatcgct gggattgttg gattcgatga agaccgtcgg
     1741 acgaaccccg agatgggctc tcgaggcatg gatcaggcgg cgggtggcca tgaacgcgag
     1801 ggcaaaaccg gtatcaccct cgtcgggctt gttgaaaccg atgcccgctc cgaattccca
     1861 gtcgtcggtg agcatgtagc ggacggcaac gggggcattg aggtcccaga aaccgatgaa
     1921 ccagtcgccc ttcttctggt cgccatccag ggcacccatg tcggcacggg ccagcgtggc
     1981 gccggcggtc gacagaaccg cggcgagcgc cgcgatcgcg agaaacttgc gcatctgtac
     2041 ctccatcaaa gaccagacta cgtgtagcgg tgaagagaat ctcaacgttc cgattcgctt
     2101 gagcgatccg gtcccggaac ttcccccctg ggatcggacg gttacggtga atcagtgcgt
     2161 ggggtctctt gcacctcctt tcgacacggt gtccgtctcg agcaggccga tgcgtgcagc
     2221 tcgcaagcca cccgccctgc atcccttcgt cgtcaaccca acccttaatg cgtggcccta
     2281 caagtgctga aacgcttgtt tcggagccca ctgtatacag gtcgaagggc ctgtcaaggg
     2341 aaaatcaagg aaaaaattcg cctggggcgg tcgggtaatc ccccatccgg gcacgaatta
     2401 ggggtgatcg ggcgtgaaaa ccgggcggag cgggggcccg ggatcagtcg cgaacgaacg
     2461 tcgacgcgat accgaggccg ccgcttacac acagcgtggc aagtccgcgg cgcacggcac
     2521 gacgacgcat ttcgtgcagc agcgtgacca cgatgcgcgc acccgaggcg ccgatcgggt
     2581 gccccagggc gatcgatccc ccgttcacgt tgagccgctc cggatcgaag cggagctcgc
     2641 gatcgacggc cagcacctgg gcggcgaacg cctcattcag ctcgacgaga tcatgatccg
     2701 cgagcgtcgt gccggtcttc tcgaggagcg cgcgcaccgc gggaacgggg ccgattccca
     2761 tgacctccgg atcg
//
LOCUS       DASVOD010000648         3177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_928272, whole genome shotgun sequence.
ACCESSION   DASVOD010000648 DASVOD010000000
VERSION     DASVOD010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928272"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF720_13950"
     CDS             complement(<1..349)
                     /locus_tag="VF720_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011188693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX7880512.1"
                     /translation="MISNLRKKMKDRRGFNMIELMVVVVIIGILAAIAVPIYSKYAKN
                     ARVTEATGRIGDILTAAKSYAIENDSDGLPTTADWPTSCGATNFVGDCTKSQNFGYTV
                     SASGGTLTILAEGD"
     gene            complement(584..1825)
                     /locus_tag="VF720_13955"
     CDS             complement(584..1825)
                     /locus_tag="VF720_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX7880513.1"
                     /translation="MPDFAYIAKDRTGSELSGIVNGASADHAIEELHKRGLVVLHVTE
                     DRTGKGDALPWYKKELSLFPSTTGTRDLALFTRQLATLFEAGIPLVRGLRGLSADETN
                     KTLSRTVKNIAERVEQGSNLSDAMAMHPRTFNSLYVSMVRAGEGSGTLEEILEHLAVY
                     LEKMDALKTKIRSAMSYPVFVLFTAVTITLFLLLKIVPTFEQIYADLDAELPLPTRVV
                     LWISKLIQNNILMTAGIVMLLIALTMLWRRTKPGRYGWDNMMLHTPVFGPIIRKVAVS
                     RFTRTFGILLKSGLPVMETLVLVRGATGNAVIEKAITDAREEIGRGREITAAFRATRK
                     IPEMVLQLMATGEESGQLDSMLMKASDFYDRQVEAAVQGLAALIEPLLIVVVGVIVGV
                     VVITMYLPIFYLGDAILHGNW"
     gene            complement(1837..>3177)
                     /locus_tag="VF720_13960"
     CDS             complement(1837..>3177)
                     /locus_tag="VF720_13960"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HEX7880514.1"
                     /translation="GLADDIAVARRKHYAELQGSLGVQEALAQATVELGPEYDDLGDS
                     QEIDSEEALRRAQDAPIVNLVNKIVTQALVERATDIHVEPLPKSLIVRFRVDGLLADA
                     LTPPRGVYTGVMTRIKILSDMDIAERRKPQDGRFTVKMNGREVDVRVSTIPTIHGEKA
                     VLRLLDKSGFDMSLTSLGFETDTLEIFRHAIHQPYGMVLLSGPTGSGKSTTLYAALLE
                     LKNQTLNITTVEDPVEYQIERINQVQVNDKKGVTFANTLRSFLRQDPDVIMVGEIRDR
                     DTADMAVRAAMTGHMVFSTIHANDAPSTAVRLTTLGAEPFQAASALTLVVAQRLMRRN
                     CTECLEPYKPPPELLSALRLDKGRAESMEFKHGKGCAVCRDRGYLGRVAIVEPLRMSP
                     RIRTLIAEGSPADAVRRVGIEEGLLTLKEAGLRKVAAGLTTVEEVLRVCLADDE"
BASE COUNT          487 a          956 c         1097 g          637 t
ORIGIN      
        1 tgtctccctc ggcgagaatg gtcaacgtgc cgccagacgc actgacggta tagccgaaat
       61 tctggctctt ggtgcagtca ccgacgaaat tggttgcacc gcagctggtg ggccagtctg
      121 ctgtcgtcgg tagcccatca ctatcattct cgatagcgta cgatttcgct gccgtaagaa
      181 tgtcgccgat gcgacctgtg gcttccgtaa ccctcgcgtt cttcgcgtac ttcgagtaga
      241 tcggcacggc gatggcggcc aggatgccga tgatgacgac gaccaccatg agttcgatca
      301 tgttgaagcc gcggcggtcc ttcatcttct tgcgcagatt gctgatcatg ggtcttttcc
      361 tccgtcttta ccttttggtg aaccttcggt gctggtgaaa tctgtgttta ccagggactt
      421 tcagtccgcg gcccggttgt ggccccggcg cggccggcgg ctgctttccg cctgggcgtg
      481 atgttcgtct ggctgttcct ccttctggct cccctgtccc ctggatgcgt acggccttgc
      541 cttacttcaa gagtgacgca gtcgaacctc tttcgttcga ctgctaccag tttccgtgca
      601 ggatggcgtc gccgaggtag aagatcggca ggtacatggt gatgacgacc acgccgacga
      661 tgacgccgac gaccacgatg aggagcggct cgatgagcgc ggccaggccc tggacggcgg
      721 cctcgacctg gcgatcgtag aagtcgctgg ccttcatcag catggaatcg agctggcccg
      781 attcctcgcc ggtggccatg agctggagca ccatttcggg aatcttcctg gtggcacgga
      841 acgcggcggt gatttcccgg ccgcggccga tttcctcgcg ggcgtcggtg atggccttct
      901 cgatcacggc gttgcccgtg gcgcccctga ccagcaccag cgtttccatg accggcaggc
      961 cggacttgag gaggatgccg aaggtgcggg tgaagcggct gacggccacc ttgcggatga
     1021 tcgggccgaa gaccggcgtg tggagcatca tgttgtccca gccgtaacgg ccgggcttgg
     1081 tgcgccgcca cagcatggtg agggcgatga ggagcatcac gatgccggcg gtcatcagga
     1141 tgttgttctg gatgagcttg ctgatccaga gcaccacgcg cgtggggagc gggagctcgg
     1201 catcgaggtc ggcgtagatc tgctcgaatg tggggacgat cttgagcagc aggaagagcg
     1261 tgatggtgac ggccgtgaag aggacgaaga ccgggtacga catggccgag cggatcttcg
     1321 tcttcagggc gtccatcttt tccaggtaga cggccaggtg ctcgaggatt tcctcgagcg
     1381 ttcccgatcc ttcgccggcc cgcaccatgc tgacgtacag gctgttgaac gtgcgggggt
     1441 gcatggccat ggcgtccgac aggttgctcc cctgctcgac gcgctcggcg atgtttttca
     1501 cggtcctgga gagggtcttg ttggtttcat cggccgagag gccgcggagc ccgcgcacca
     1561 gcgggatgcc ggcctcgaag agcgtggcca gctggcgagt gaagagggcc aagtcgcgcg
     1621 tcccggtggt gctggggaag agcgagagtt ccttcttgta ccaggggagc gcatctccct
     1681 tgccggtgcg atcctcggtg acgtgcagga cgacgaggcc gcgcttgtgc agctcctcga
     1741 tggcgtggtc ggcggaagcg ccgttcacga ttcccgagag ctcggagccc gttcgatcct
     1801 tggcgatata tgcgaaatcc ggcacggctt atcctctcat tcgtcgtctg ccaggcagac
     1861 gcgaagcact tcttccaccg tcgtgaggcc ggcggccacc ttgcggagac cggcctcctt
     1921 gagcgtgagc aacccttcct cgatcccgac acggcggacg gcgtcggccg gggacccttc
     1981 ggcgatcagg gtgcggatac ggggcgacat gcggagcggt tcgacgatgg ccacgcgccc
     2041 gaggtatccc cggtcgcggc atacggcgca ccccttgccg tgcttgaact ccatcgactc
     2101 ggcccgcccc ttgtcgagcc ggagggccga caggagctcg ggaggcggct tgtacggctc
     2161 gaggcactcg gtgcagttac ggcgcatgag gcgctgcgcc accaccaggg tgagggcgga
     2221 ggccgcctgg aagggttcgg ctcccagcgt ggtgaggcgg accgccgtgg agggcgcgtc
     2281 gttggcgtga atggtggaga agaccatgtg gccggtcatg gccgcgcgga ccgccatgtc
     2341 ggccgtgtcg cggtcgcgga tctcgcccac catgatgacg tcgggatcct ggcgcaggaa
     2401 gctccggagc gtgttggcga aggtgacgcc cttcttgtcg ttgacctgga cctggttgat
     2461 ccgctcgatc tggtattcga ccgggtcttc cacggtggtg atgttcagcg tctggttctt
     2521 gagctcgagg agcgccgcat acagggtcgt ggactttccg gacccggtcg ggcccgacag
     2581 gagcaccatg ccgtacggtt ggtggatggc gtgccggaag atttccagcg tgtccgtttc
     2641 gaatcccagg ctggtgagcg acatgtcgaa gcccgacttg tcgagcaacc ggaggaccgc
     2701 tttctcgccg tggatcgtcg ggatggtgga gacgcggaca tcgacctcgc ggccgttcat
     2761 cttgacggtg aaccggccgt cctgcggctt ccgccgttcg gcgatgtcca tgtcggagag
     2821 aatcttgatg cgcgtcatca cgccggtgta gacgccgcgc ggcggcgtga gcgcgtcggc
     2881 caggaggccg tccacccgga accggacgat gaggctcttc ggcagcggct cgacatggat
     2941 gtcggtggcg cgctcgacca gggcctgcgt gacgatcttg ttgacgaggt tgacgatggg
     3001 cgcgtcctgc gcgcggcgga gcgcctcctc ggaatcgatc tcctgcgagt ctcccaggtc
     3061 atcgtactcg gggcccagct cgacggtggc ctgtgccagc gcctcctgca ccccgaggct
     3121 tccctgcagc tcggcgtagt gcttgcgccg ggccaccgcg atgtcgtcgg ccaggcc
//
LOCUS       DASVOD010000649         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_512150, whole genome shotgun sequence.
ACCESSION   DASVOD010000649 DASVOD010000000
VERSION     DASVOD010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512150"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VF720_13965"
     CDS             <1..570
                     /locus_tag="VF720_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7880515.1"
                     /translation="QKLEPRCQAGTIFATNTSALSITEMAAATGRPHGVIGMHFFNPV
                     PRMKLLEIVRGLETSDATVEACRTVGEKMGKEIAVVSESPGFITSRINAMIGNEAFYM
                     LESGVASASEIDRALKLGLNHPMGPFEMVDLVGLDVRLSILNYLHSALGEKFRPCPLL
                     VKYVKAGRFGRKVGRGVYDYDAAGNRIGS"
     gene            657..1430
                     /locus_tag="VF720_13970"
     CDS             657..1430
                     /locus_tag="VF720_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX7880516.1"
                     /translation="MSANIRVETRHHVATLTIDRPAVRNALDRATADEISAALAELRR
                     DESLRVLVITGGGDRVFVSGADIKDLKSRTFQQGLEGCNNALFAAIETFERPVVAAIN
                     GHALGGGLELAMACDIRIAVDDAKFGFPEVGLGIMPGAGGTQRLPRLVGTGKAKELIL
                     TGDLIDAPEAHRIGLINRLVPRGEFSAGVTSLVDKLASRGPMALKLAKMSLNAAARMP
                     IDAGFQLEILAQSVLFETKDKDEGLNAFLEKRAPKFEGR"
BASE COUNT          273 a          492 c          544 g          242 t
ORIGIN      
        1 cagaagctcg agccgcggtg ccaggccggc accatctttg ccacgaacac ctcggcgctc
       61 tcgatcaccg agatggccgc cgccaccggc cgcccgcacg gcgtcatcgg catgcacttc
      121 ttcaaccccg tgccgcggat gaagctgctc gaaatcgtgc gagggctgga gacgagcgac
      181 gccaccgtcg aggcctgccg gaccgtgggc gagaagatgg ggaaggagat tgcggtagtg
      241 agcgagagcc ccggcttcat caccagccgc atcaacgcca tgatcggcaa cgaggccttc
      301 tacatgctcg agagcggcgt ggcctcggcg tccgagatcg accgcgcgct gaagctcggg
      361 ttgaatcatc cgatgggacc gttcgagatg gtggacctgg tggggctcga tgttcggctt
      421 tcgatcctca actacctgca ctcggcgctc ggcgagaaat tccggccgtg tcccctgctg
      481 gtgaagtacg tgaaggcggg gcgcttcggg cggaaggtgg ggcgcggggt gtacgattat
      541 gatgccgcgg ggaatcggat cgggagctag gacaggagcc acagtgtcat cacgaatcgg
      601 tgccgacgag ccgcaggacg gactgagagt tgaaaaacca cccctgaaag gcacccgtgt
      661 ccgccaacat ccgcgttgaa acccgccacc acgtcgccac gctcaccatc gaccgccccg
      721 cggtgcgcaa cgcgctcgac cgcgccacgg cggacgaaat ctcggcggcg ctggcggagt
      781 tgcggcgcga cgaatcgctc cgcgtcctgg tcatcaccgg cggcggggat cgcgtgtttg
      841 tctcgggtgc cgacatcaag gacctgaaga gccgaacctt ccagcagggg ctggaaggtt
      901 gcaataacgc cctgttcgcc gccatcgaga cattcgagcg gccggtcgtg gcggccatca
      961 acggccacgc gctcggcggt ggcctcgaac tggcaatggc ctgcgacatc cgcatcgcgg
     1021 tggatgacgc gaagttcggg ttcccggagg tggggctcgg catcatgccc ggcgccggcg
     1081 gcacgcagcg tctgccgcgg ctcgtcggca cggggaaggc caaggagctg atcctcaccg
     1141 gcgatctgat cgatgcgccg gaggcgcacc gcatcgggct catcaatcgc ctggtgccgc
     1201 gtggcgagtt cagcgccggg gtgacttcgc ttgtcgacaa actggcgagc cgcgggccga
     1261 tggccctgaa gctggccaag atgtcgctga acgccgccgc gcggatgccg atcgatgccg
     1321 gcttccagct cgaaatcctg gcgcagtcgg tgctgttcga gacgaaggac aaggacgaag
     1381 gactgaacgc gtttctcgag aagcgggcgc caaagttcga ggggcgctga cgcggggcgc
     1441 gtccgtcgga acgattcctg tcgggagagg ggtggccctt tcgggtcggc cgtcgcaggt
     1501 gacttgccgg gttgccccgc cccgccgaga attccggcca tgagccaccg g
//
LOCUS       DASVOD010000650         4224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_928489, whole genome shotgun sequence.
ACCESSION   DASVOD010000650 DASVOD010000000
VERSION     DASVOD010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928489"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1588
                     /locus_tag="VF720_13975"
     CDS             <1..1588
                     /locus_tag="VF720_13975"
                     /inference="COORDINATES: protein motif:HMM:NF039776.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880517.1"
                     /translation="NAEAGFIQHHSNGLLAFGQFVDPENDGNAGATSTNPPNQDECVG
                     GTDAGLIAPSAYTIDDGSTMPCDPLSTTPLGGGCGTVTVGPNLDIAIRNINQGTCYFN
                     LLIDLDYSGSWGGPTQCPLGGDTDEHVFQNIVVPNNFSGELSDLLPGATFTITSPPGY
                     TWARFTLTSVPVPVDWDGSGDFANGETEDYLFRIEGPGLGDYGDAPENALAYPNSGYW
                     GAFATCAADGFPHFVYHRPQGNVWIGATKDFETEGNGGYCTIENQVPDQDECGNNVDG
                     GLVNPDAYTIDANGWDVPCEANQLRPLGQPCTVGTIGPSLNIRLHNGEATSMRFNLLV
                     DWNQDGYWYGLGDCGTYQEDEHLIRNVEVPAGFDGLLSTLTGPTTFNIPSAEGTVWVR
                     ATIGENFVSDDWNGFFVFNQGETEDYKLAIINDVSGIPDGGTKPRVLHLGPAFPNPSA
                     GLTAIRYTLPADGPVRLAIFDISGRAIASLDIPHAEAGQHSWEWDGRDELGRLTPAGI
                     YVVKLESGGVTQSMKVTRVR"
     gene            1768..1980
                     /locus_tag="VF720_13980"
     CDS             1768..1980
                     /locus_tag="VF720_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880518.1"
                     /translation="MKDHDGKPVLFWETLKTKREEDGLFDSKYLVELAKVPGGWLVIS
                     QFLVGPGLGSVFLPDPDHKWDGGSLR"
     gene            complement(1984..2898)
                     /locus_tag="VF720_13985"
     CDS             complement(1984..2898)
                     /locus_tag="VF720_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880519.1"
                     /translation="MTRFATALALLLLAFLSQASAADAPWGRPLDGLSCRLSGPSEVR
                     QGDTLMVVLELRHDGANHGTITHIVDWPTWYCTTLEIVGADGRVHERRWPTGGRLIVP
                     GHCTNNRWRDVGTPVDPDSFPVPTLGDDGDQLASGRYRVTAVYENRQEHIGGSSPNQP
                     DTASIWSGRIRSEPITLDVTAGAEETVSFVAYTGVRRVVLDGKSAWEWDPATRQLMTA
                     SRRPGYHLGRILSLDGNRGGCGFSNATSGGLKEIDCEGSYLSQASPDTIRIYQWVGET
                     SHADTHMWHPQSGDFRAFFESTIVIPPE"
     gene            complement(3031..>4224)
                     /locus_tag="VF720_13990"
     CDS             complement(3031..>4224)
                     /locus_tag="VF720_13990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7880520.1"
                     /translation="VPVWGTRMATGPGRVYTFGRTGGQAVLGIVDAGAPEAPIWKSSI
                     QVSSNANTVGIQAVGNRVYVHTSRWFSSGQSRLAIVDVTNPVAPNILGTLPTLGGGSL
                     AVVGNYAYVAQNGPSIAVVDISIPSTPALISETPCAGLGEGVAYDNGYLYLATESEGL
                     QIFDLSVPHAPAFVASFPLPWAYEVTVRDHVAYVSGGDVFMIDVSNPVAPSLLSMLAI
                     DGSAGAIRFNGAHAWITTDVDVIVVDMSNPASPRRLGEQAGNGEVYDDLGIINDLVYT
                     CGSDGTIHVLPAQCTVTAVPETPIAPTAARLRVLPPFPNPTTDGAGFIARLPEAGRYK
                     IAIHDISGRLVTDLPSIEAPGPMDASLAWNGRDHDGKAVATGVYFVRVHGRGETATQR
                     LLVTR"
BASE COUNT          765 a         1397 c         1351 g          711 t
ORIGIN      
        1 gaacgccgag gccggcttca ttcagcacca ctcgaacggc ctgctcgcct tcggccagtt
       61 cgtggatccg gaaaacgacg gcaatgcggg cgctaccagt acgaacccgc cgaaccagga
      121 cgagtgcgtg ggcggcaccg atgccgggct catcgccccg tcggcctaca cgatcgacga
      181 cggatccacg atgccgtgcg atccactctc cacgacaccg ctcggcggtg gttgcggaac
      241 ggtgacggtc ggtccgaacc tcgacatcgc catccgcaac atcaaccagg ggacgtgcta
      301 cttcaacctg ctgatcgacc tcgactactc gggttcgtgg ggcggcccca cgcagtgccc
      361 gctcggcggc gacaccgacg agcacgtgtt ccagaacatc gtcgtgccga acaacttcag
      421 cggcgagctc tcggacctgc tccccggcgc cacgttcacg atcacctcgc cgcccggcta
      481 cacgtgggcc cgcttcacgc tgacgtcggt cccggtgccc gttgactggg acggctccgg
      541 cgatttcgcc aacggcgaaa ccgaggacta cctcttccgc atcgagggcc ccggcctcgg
      601 tgactacggc gacgcgcccg agaacgcgct cgcctatccc aattcgggct actggggcgc
      661 cttcgccacc tgcgccgccg atggcttccc gcacttcgtg taccaccgcc cgcaggggaa
      721 cgtgtggatc ggggccacga aggacttcga aaccgaaggg aacggcggct actgcaccat
      781 cgagaaccag gtgcccgacc aggacgagtg cggcaacaat gtggacggcg gcctcgtgaa
      841 tcccgacgcc tacacgatcg acgccaacgg ctgggacgtg ccgtgtgaag ccaaccagct
      901 ccgaccgctc ggccagccgt gcaccgtcgg cacgatcggg ccgtcgctca acatccgcct
      961 gcacaacggc gaagccacct cgatgcgctt caacctgctg gtggactgga accaggacgg
     1021 ctactggtac ggcctggggg attgcggcac gtaccaggag gacgagcacc tcatccgcaa
     1081 cgtcgaggtg cccgccggct ttgatggcct cctctccacg ctcaccggcc cgaccacgtt
     1141 caacatcccg tcggccgagg gcacggtgtg ggtgcgcgcc acgatcggcg agaacttcgt
     1201 gtcggacgat tggaatggat tcttcgtctt caaccagggc gaaaccgagg actacaagct
     1261 ggcgatcatc aacgacgtca gcggcattcc cgacggcggc acgaagccgc gggtgctcca
     1321 cctcggcccg gccttcccga atccgtcggc cggtctcacc gccatccgct acacgctccc
     1381 ggccgacggc ccggtccgcc tggcgatctt cgacatctcc ggtcgcgcca tcgcctcgct
     1441 cgacatcccg cacgccgaag ccggccagca cagctgggaa tgggatggcc gggacgagct
     1501 cggccgcctc accccggccg gcatctacgt cgtgaagctg gagtcgggtg gggtgacgca
     1561 gtcgatgaag gtgacgcggg tccggtaacc ctgcgcagcg gcaacagctg attcaccgcc
     1621 cgggcggagt caatcgcgtg tgctggaaca tgcggtgggc ttcgcccggg attgtttaca
     1681 atctggtcgg tccctgcgcc gtgccgtcga aaacggcgcc atcgggacga ctcaagagca
     1741 tgacgattct cacctgaggg agggatcatg aaagatcacg acggcaagcc ggtgctcttc
     1801 tgggaaacgc tcaagaccaa acgcgaggag gacggcctct tcgactcgaa gtatctcgtc
     1861 gagctggcga aggtgccggg tggatggctc gtgatctctc aattcctcgt aggccctggc
     1921 ctcggaagtg ttttccttcc cgacccggat cacaagtggg atggtgggag tctgcggtag
     1981 gggtcactcc ggcgggatga cgatcgtgct ctcgaagaag gcgcgaaagt ctcccgactg
     2041 cgggtgccac atgtgagtgt cggcgtgcga cgtttcgccg acccactggt agattcggat
     2101 cgtgtccggt gatgcctggc tgaggtagct gccctcacaa tcgatttcct tcagtccccc
     2161 ggatgtcgcg ttcgaaaacc cgcacccccc gcgattacca tcgaggctca agatgcgacc
     2221 caggtggtag cccggacgcc gcgatgccgt catgagttgc cgggtagccg gatcccactc
     2281 ccatgccgac ttgccgtcca acacgacccg ccgtacgccc gtgtacgcca cgaagctcac
     2341 ggtttcctcc gccccggccg tgacgtcgag cgtgatcggc tcggaccgga tccggccgct
     2401 ccagatggac gcggtatccg gttggttcgg actgctcccg ccgatgtgct cctgacggtt
     2461 ttcatagaca gctgtgaccc ggtagcggcc ggaggcgagc tggtcaccgt cgtcgcccag
     2521 cgtcggaacc gggaaggagt cgggatcgac cggtgtgccc acgtcgcgcc accggttgtt
     2581 cgtgcagtga cccggcacga tcaaccggcc gcccgtgggc cagcgcctct catgcacgcg
     2641 cccatccgcc ccgacgatct ccagcgttgt gcagtaccac gtcggccaat cgacgatgtg
     2701 cgtgatcgtg ccgtggttcg cgccgtcgtg acgtaattcc agcaccacca taagcgtgtc
     2761 gccctggcgc acttccgagg gccccgacag gcggcacgac agcccgtcga gcggtcgccc
     2821 ccagggtgcg tctgcggcgg aggcctggga cagaaaggcc agaagaagca gggccagtgc
     2881 ggtggcgaat cgtgtcatcg gactcctgtt tcgggatcga ggggggatcc gatggggtga
     2941 ggtttggatc ggggcctgaa aggaatatag cgatgaagaa ggatcccgcg ccaacggcgc
     3001 gggaaactat tggcccttcg acatcgtcgg ttaccgcgtt accagcagcc gctgcgtggc
     3061 ggtttcacca cggccgtgaa cgcggacgaa gtaaacgccg gttgccacag ccttgccgtc
     3121 atggtcgcgg ccgttccagg ccagtgaggc atccatcggg cctggagcct cgatgctcgg
     3181 caggtccgtc acgagccgcc cactgatgtc gtgaatcgca atcttgtagc gccccgcttc
     3241 cggaagtcgt gcgatgaagc cggcaccgtc cgtcgtcgga ttcggaaagg ggggcagtac
     3301 tcggagccgt gcggctgttg gcgcgatagg cgtttccgga acggcggtga ccgtgcactg
     3361 cgcaggcagg acgtggatcg tcccatcgga tccgcaggtg tagacgaggt cattgatgat
     3421 gccgaggtcg tcgtacactt caccatttcc ggcctgttcc ccaaggcgac ggggcgaggc
     3481 ggggttcgac atgtcaacaa cgatgacatc cacgtcggta gtgatccacg cgtgggcgcc
     3541 gttgaagcgg atggcccctg ccgaaccatc gattgcgagc atcgacagaa gtgacggcgc
     3601 cacagggttc gacacgtcga tcatgaaaac gtcgccgccg gacacataag ccacatgatc
     3661 acgaacagtt acttcgtatg cccaaggcag gggaaagctc gccacgaatg ccggcgcgtg
     3721 gggcacgctg aggtcgaaaa tctgcaaccc ctcggactcc gtggccaggt agagatatcc
     3781 attgtcgtaa gccacgcctt ccccgaggcc cgcacacggg gtctcgctga ttagcgccgg
     3841 tgtagacggg atggagatat cgacgaccgc gatgctgggg ccgttctgtg caacataggc
     3901 atagttcccg acgaccgcca gtgagccccc tcccagggtg ggaagtgtcc caaggatgtt
     3961 tggcgccacg ggattggtga catcgacgat ggccaggcgg ctctgaccgg acgagaacca
     4021 acggctcgta tgcacataca cccggttgcc gacggcctgg atgccgaccg tgttcgcgtt
     4081 gctggacacc tggatggacg acttccatat cggcgcttcg ggcgcacctg catcgacgat
     4141 acccaggacc gcctggcccc cggtgcggcc aaacgtgtac acgcggccgg gccccgtcgc
     4201 catcctggtt ccccacaccg gcac
//
LOCUS       DASVOD010000651         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_460865, whole genome shotgun sequence.
ACCESSION   DASVOD010000651 DASVOD010000000
VERSION     DASVOD010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460865"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1734)
                     /locus_tag="VF720_13995"
     CDS             complement(52..1734)
                     /locus_tag="VF720_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX7880521.1"
                     /translation="MSVSDRDSESIPAPAAPGTVPPATPGTATPPGQVALDRFRKKAK
                     ALLESAQNGDAAVLARLAAVPRLKDTPPSDLATMVQLADAQYVVAREAGAESWPKLKA
                     MLDALDPETVAAGQFLLLLADRQFEKARAILAAHPAIPGQNLFTAAATGDLAALEKHL
                     AAATSPSPTYPHNKWTPLLYVAGSPFHADAPDGPERFAEAARRLLDAGASANEHTIWT
                     EDEGKSTLSALYRAVIGNNVPVARLLLERGANPNDGESIYHGAELNHVAMLELLLAHG
                     AEISKPDPHWGNTPLYFLSGYFEGAPPSAILGMRWLLEHGADPNQPSGENAETPLHQL
                     VRNHREPEVLALLLDHGADPNAARADGITPYRLAVQSGHDSAARLLESRGAIPEGVTA
                     FDRFLGAAMDGRTAEARGFLDRGEVSLADLTGEQRGLLAHAANAPSMAAVDALLDVGF
                     DLAWESEWGGTALHWVAWHGKVEMVKHLLERGAPVNIRDKRFGSSPLAWAGHGSQNCR
                     QADDDYMAIIDLLLAAGSDRETSFNGWNEPPEALSSARVAAHLKKRLAATTS"
     gene            1981..>3671
                     /locus_tag="VF720_14000"
     CDS             1981..>3671
                     /locus_tag="VF720_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014216345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880522.1"
                     /translation="MLAWRMHKDAKTVTAPLANAAPPPIPDTNRPRNADDHADGNRAR
                     RKLKIGILDLVHKGPTTALWARLMHANLASFMPQALAVWCEEAGHEVTFAVYTGLENV
                     ESMLPDDVDLVLIGAFTQAAQLSYALSNMYRKRGAVTVLGGPHARCYPEDAARYFDYV
                     LGFTDKETLDQVLREAAPHRPMGVCISAKKQPTYLPSLEQRWKFVAPTLRKAISPIKM
                     VPMLGSLGCPYTCSFCIDSEIEYQPFEFSRVGDDLRFLVKTMPGVWVAWHDPNFGVRF
                     DEMLAEIDSAVPRGKLSFAAESSLSLLSEPRLKRLKDAGFVAMLPGVESWFSLGNKSK
                     TGGRQGLEKVEQVSDHINMIMRYIPYVQANFVLGLDVDAGDEPFELTKRFLRRTPGVF
                     PGYSLMSAFGEAAPLNLELQRAGRVLPFPFHFLNNNEAMNVKPKNYEWVEFYDRIIDL
                     VGYSFSPGTMARRVKANWGSFAWWGNIVRGISSEGYGRLHYHSRIRGLLKTDATMRDF
                     FEGATDVIPGFYRDRVREELGPLWQHLPEGALDHDPTAYLKKSDARCETVVRARAA"
BASE COUNT          587 a         1228 c         1250 g          606 t
ORIGIN      
        1 tggcaactga actggggcgg ggtgaactag gcgctcgacg gccggcctcg atcaggacgt
       61 ggttgccgcc agcctcttct tgagatgcgc cgccacgcgc gcggaggaga gcgcttccgg
      121 cggctcgttc cagccgttga acgacgtctc gcgatcgctg ccggcggcaa gcagcaggtc
      181 gatgatggcc atgtagtcgt cgtcggcctg gcggcagttc tgcgagccgt gcccggccca
      241 ggccagcggg gagctgccga agcgcttgtc gcggatgttg accggcgcgc cccgctcgag
      301 gaggtgcttc accatctcca ccttgccgtg ccaggccacc cagtggagcg cggtgccgcc
      361 ccactcgctc tcccaggcca ggtcgaagcc gacgtcgagc agcgcatcga cggcggccat
      421 gctcggggca ttggcggcgt gcgcgaggag cccgcgttgt tcgcccgtca ggtcggcgag
      481 cgatacctcg ccgcgatcga ggaatccacg ggcctccgcg gtgcgaccgt ccatggcggc
      541 gccgaggaac cggtcgaagg ccgtcacacc ctccggaatc gcgccgcgcg actcgaggag
      601 ccgggccgcg ctgtcgtgac cggactgcac cgccagtcga tagggcgtga taccgtccgc
      661 gcgcgccgcg ttcgggtcgg cgccatggtc gaggaggagc gcgagtacct ccggttcgcg
      721 atggttccgg accagttggt ggaggggcgt ctccgcgttc tcgccgctcg gctggttggg
      781 atcggcgcca tgttcgagga gccagcgcat ccccaggatg gcggaagggg gcgccccctc
      841 gaaatacccg gacaggaagt agagtggggt attcccccag tgcggatccg gcttgctgat
      901 ctcggcgccg tgcgcgagca gcaactccag catcgccacg tggttcagtt cggcgccgtg
      961 gtaaatcgat tcgccgtcgt tggggttggc cccccgctcg aggaggagcc gggccacggg
     1021 gacgttgttg ccgatgaccg cccggtagag cgccgacagc gtgcttttcc cctcgtcctc
     1081 cgtccagatc gtgtgctcgt tggccgaggc accggcatcg aggagccggc gggccgcctc
     1141 ggcgaaacgc tccgggccgt ccggcgcatc ggcatggaag ggcgagccgg ccacgtagag
     1201 gagcggcgtc catttgttgt ggggataggt gggcgacggg ctcgtggccg ccgcgaggtg
     1261 cttctccagc gccgcgaggt cgccggtcgc cgccgcggtg aaaaggttct gccccgggat
     1321 ggccggatgc gcggccagga tggcccgggc cttctcgaac tgccggtcgg cgaggaggag
     1381 gaggaactgg ccggcggcca cggtctccgg gtcgagggcg tccagcatgg ccttgagctt
     1441 cggccagctc tcggcgccgg cctcgcgcgc gacgacgtac tgcgcatcgg ccagctgcac
     1501 catcgtggcc aggtcggagg gcggggtgtc cttcaggcgc gggacagcgg ccaatcgggc
     1561 caggaccgcg gcgtcaccgt tttgcgcgga ctcgaggagc gccttcgcct tcttgcggaa
     1621 tcggtcgagg gccacctggc cgggcggggt tgctgtgccc ggtgtggcgg ggggtacggt
     1681 gccgggtgcg gcgggggcgg gaattgattc tgaatcgcga tctgatacgg acatgacacg
     1741 tcctccgtca ggccccgtgt ccgccacacc gggtaggaga aacgtgttgg ctgatcttga
     1801 tcattccagg caggtgggac tgaacccttt ccgcggaccg gcggcgccct gagcgcgcat
     1861 agaactctat cacacacagg attgttcagg aggacgcacg aaaataccca tcggaaaata
     1921 tcccaatcgg gagaatcctt ccgatttagg gccggcagcc ctattttcga acacccgtcc
     1981 atgctagcgt ggcgtatgca caaagacgcg aagaccgtaa ccgcccccct cgcgaacgcc
     2041 gccccacccc ccatccccga caccaaccgc ccacggaacg ccgatgacca tgccgacggc
     2101 aaccgtgcgc gccggaagct caagatcggg atcctggacc tggtgcacaa ggggccgacg
     2161 accgcactct gggcgcgcct catgcatgcc aacctggcca gcttcatgcc ccaggccctg
     2221 gccgtctggt gcgaggaggc cggacacgag gtgaccttcg cggtctacac aggcctcgaa
     2281 aacgtggaat cgatgcttcc cgacgacgtg gaccttgtcc tgatcggggc cttcacccag
     2341 gcagcccagc tgtcgtacgc gctctccaac atgtaccgga agcgcggcgc ggtcacggtt
     2401 ctcggcgggc cccacgcccg ctgctacccg gaggatgccg cccgctactt cgattacgtc
     2461 ctcgggttca ccgacaagga gacgctggac caggtgctcc gcgaagccgc cccgcaccgc
     2521 ccgatggggg tgtgcatctc ggcgaaaaag cagccgacat acctccccag cctcgagcag
     2581 cgctggaagt tcgtggcccc caccctgcgg aaggccatct cccccatcaa gatggtgccg
     2641 atgctgggaa gcctcgggtg cccctacacc tgcagcttct gcatcgactc cgaaatcgag
     2701 taccagccct tcgagttctc gcgggtcggg gacgacctcc gcttcctggt caagacgatg
     2761 ccgggcgtgt gggtggcctg gcacgatccg aacttcggcg tccgtttcga cgagatgctg
     2821 gccgagatcg acagtgccgt tccgcggggc aagctctcct tcgcggccga aagcagcctg
     2881 tcgctcctct ccgaaccgcg cctcaagcgc ctcaaggacg ccggcttcgt ggccatgctt
     2941 cccggggtcg agtcctggtt ctcgctcggc aacaagtcga agacgggcgg gcgccaggga
     3001 ctcgaaaagg tggagcaggt ctccgatcac atcaacatga tcatgcgtta catcccgtac
     3061 gtgcaggcca atttcgtgct ggggctcgac gtggatgccg gcgacgagcc gttcgagctc
     3121 accaagcgat tcctccggcg gacgccgggc gttttccccg gctactcgct gatgtcggcc
     3181 ttcggcgagg cggcgccgct caacctcgag ctgcagcgcg ccggccgcgt cctccccttc
     3241 ccgttccatt tcctcaacaa caacgaggcg atgaacgtga agcccaagaa ctacgagtgg
     3301 gtggaattct acgaccggat catcgacctg gtggggtatt cgttctcgcc gggaaccatg
     3361 gcgcggcgcg tgaaggccaa ctggggctcc ttcgcctggt ggggcaacat cgtccgcggc
     3421 atttcatccg aggggtatgg gcgccttcac taccactcgc ggatccgcgg cctgttgaag
     3481 accgacgcca ccatgcgtga tttcttcgaa ggcgccacgg acgtgatccc cgggttctat
     3541 cgcgaccggg ttcgggaaga actggggccg ctctggcagc acctgccgga aggggcgctc
     3601 gaccacgacc cgaccgccta cctcaagaag tcggacgcgc gttgcgagac ggtcgtgcgt
     3661 gcccgggcgg c
//
LOCUS       DASVOD010000652         9124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_719619, whole genome shotgun sequence.
ACCESSION   DASVOD010000652 DASVOD010000000
VERSION     DASVOD010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719619"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF720_14005"
     CDS             <1..196
                     /locus_tag="VF720_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012509699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX7880523.1"
                     /translation="YTGMGGPLFLRAYAFDVDETTGYILGGSRGREEAPDEGKFTLTL
                     TREAGGPWLIMSDMDNHNTR"
     gene            193..1146
                     /locus_tag="VF720_14010"
     CDS             193..1146
                     /locus_tag="VF720_14010"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX7880524.1"
                     /translation="MIIGILGAGAVGGYYGAMWHRAGHNVRLLARGAHGAAIARSGLS
                     VKSSFGEVTIPPSQLVTDIARETPVDVLVVCVKLRDTESACRSVAPWVGPRTIVMSLQ
                     NGVDKDDMLDNVFGPERVAGAVTYIVATIEEPGVVGHKGNIARIVAGVRTSGTAPGLS
                     LALDSMRVPGVEVVAAEDVRQETWRKFIFLSTLSGMTAVTGRPIGELRDDPETRQRLG
                     DMVAEGANVAAAEGIEFDQEFVEDSMEFIDKLPADGRSSMAVDLSRGQPLELPWLSGA
                     MARRAERLGVPAPTHRRIAEVLAPYADGGGDRYRPAADLWS"
     gene            1250..1522
                     /locus_tag="VF720_14015"
     CDS             1250..1522
                     /locus_tag="VF720_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015735421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX7880525.1"
                     /translation="MCRNIKTLFNFDPPATDAEIHDASLQFVRKLSGFTAPSKANEAA
                     FNQAVEETARVARRLIDALVTNAEPKNRDEEAAKAKARAAVRFGSN"
     gene            1530..2006
                     /locus_tag="VF720_14020"
     CDS             1530..2006
                     /locus_tag="VF720_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX7880526.1"
                     /translation="MAEYRAVTEWRCGDQPFIDNRYSRAHRWTFDEGVSVTASASPLH
                     VPLPLSVGAAVDPEEAFVASLSSCHLLTYLYLAAKKGFRVESYRDEAVGRMEKGEDGG
                     LWMSVVTLRPVVTFGGDRRPSAAEAEALHHDAHAECYIARSVRSEVRCEATSLGVA"
     gene            2003..2392
                     /locus_tag="VF720_14025"
     CDS             2003..2392
                     /locus_tag="VF720_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding ACT domain-containing
                     protein"
                     /protein_id="HEX7880527.1"
                     /translation="MKDLAIRLDNSPGALAAMGEALGRAGVSIEGGGAWVVDGVGVAH
                     FLFEDGAAARRALEAAGITVLAEREVVVQRLKQAVPGQLGQLTRRMADAGVNIEVLYS
                     DHDHQLILVVDDVTTGRTVAEAWSREG"
     gene            complement(2415..3182)
                     /locus_tag="VF720_14030"
     CDS             complement(2415..3182)
                     /locus_tag="VF720_14030"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7880528.1"
                     /translation="MTTGPRKFHDFCWTNMLTPQPARAQEFFTKVLGWSYGEIPGMGH
                     LLKVDGHNMGAMFDTEGQFSMQGAPPHIGVLIKVADVDATAARVNALGGKAQAPFDVG
                     PNGRMAVCFDPSGANFDVWQEGQQATRTDDSTHHGAASWFECLTTDPAACVSFYTELF
                     GWTTRVMSMPGFEYTVFALDGAPVAGCMILMPESGGTKPHWSTVFTIDDADKAAALTA
                     ELGGTVIMPPHDVPGVGRASVLKSPQGVVFQVIKYSS"
     gene            complement(3179..3886)
                     /locus_tag="VF720_14035"
     CDS             complement(3179..3886)
                     /locus_tag="VF720_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX7880529.1"
                     /translation="MTTKERVADALAWLEKRGTKRTRDGMARYGITAKNAYGVPVGTI
                     KQLGKQLGRDHGLALALWKTGVYEAQLLTAFVGDPARVTVAQMNAWSKDFDNWGVVDT
                     LCFNFLDLSPLAWKVVGPWSRRKDEFQKRGAFALLACLAAHDKASGDAPFLASLAIIE
                     AGATDERNFVKKGVSWALRLVGRRSPAVYAASISLAEKLAASDNPTARWVGKDALRDL
                     MKPAVRKRLAATKGKKS"
     gene            complement(3883..4368)
                     /locus_tag="VF720_14040"
     CDS             complement(3883..4368)
                     /locus_tag="VF720_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7880530.1"
                     /translation="MMTVLKPGVTDYATPNDTDIVVTRVLKAPRALVFDAWTSPKHLP
                     NWMTGPDGWTMPVCEVDLKPGGAWRYVWRKDDGAEMSMSGFFREVSPPDRVVSTESWG
                     PEWPETLNTVVFTESAGYTTATLTMHFASKETRDAALKTGMKEGMDMGFARLDTLLAA
                     A"
     gene            complement(4352..4717)
                     /locus_tag="VF720_14045"
     CDS             complement(4352..4717)
                     /locus_tag="VF720_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7880531.1"
                     /translation="MHAASDTLSLTLSALADPTRRAILVRLARGSATVKELSAPFDMT
                     GPAVSKHLKVLEKAGLIQRGLKAQWRPRTLEAAPLKEVAEWTGEFRRFWDASYRNLDT
                     YLDQLQREPDKETDHDDRT"
     gene            4867..5772
                     /locus_tag="VF720_14050"
     CDS             4867..5772
                     /locus_tag="VF720_14050"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX7880532.1"
                     /translation="MSPEYDVIIVGAGTAGLSAALILGRARRRVRLLDWGSPRNAPAR
                     TSNGVFTRDGTPPDELARLACAQLAPYETVSVTRVPALGARELDGGFEVNLEGGQVIT
                     CRKLLLAHGVVDEMPAIPGVPELWGSAVFHCPYCDGWEVRDQPLAFHPNGDGEAALNM
                     ARLLLCWSRDLVLCTNGQTLLSKDDRRMLERWGVGVREEPMVRIEACDTEAAQIVFQG
                     GGALARRALVLHPHQHLACDMALTLGCTLDDKGLLSVDPRGETAVPGVYAAGDIITGI
                     QMVIRAAASGATAAVGINNALALDV"
     gene            5857..6213
                     /locus_tag="VF720_14055"
     CDS             5857..6213
                     /locus_tag="VF720_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEX7880533.1"
                     /translation="MPYVDGFVLPVPVKNLEAYRRMSRKAGKVWMEHGALEYRECAGD
                     DLNLKMAVNFPKMAKVKKGETVVFSWIVYKSKAHRDRVNKKVMVDPRITSIDPRTMPF
                     DWKRMMYGGFKTIVDM"
     gene            6227..6604
                     /locus_tag="VF720_14060"
     CDS             6227..6604
                     /locus_tag="VF720_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX7880534.1"
                     /translation="MSDPNDFPYETHLDIRYPPLAVVDVPALEAAVAHPWYNQTLCRV
                     NGSVVRLGVMQGEYHWHKHDDDDEFFFVLEGRFLIDLEEETIDLGPKQGYVVPKGVVH
                     RTRAPERAVILMVENAGIIPTGD"
     gene            6607..8940
                     /locus_tag="VF720_14065"
     CDS             6607..8940
                     /locus_tag="VF720_14065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX7880535.1"
                     /translation="MIFREKPGAILAYGITVAAVLAAAIGRLLIDPVVGGSVPFITLF
                     PAVVVAAWIGGFGPAFLATVLGFVFSLIYFSPPRFQFHDASRAQEVSLTLYVLVSLTI
                     AGFGEAMWRARRRSQDAAAEQLRSSHLLRITLASIGDAVVTTDDAGRVTYLNHVAEEM
                     TGWTVDEAVGRPLPEVFPILDEATQVSAESLVGRVLRGGNAVPLSGQSLLARKDGTMR
                     PVEESAAPIRNDDGSVVGVVLVFHDIAERRAAEKALRDREQRFRDMANHITPIVWTCD
                     ASGRATWVNERWYEYSGTTFDEMTGVTGKDILHPDHRERVRGGLRNAFSEGRHWEDTF
                     PLLGRDDRYRWFLSRAVPIRDDEGRVLQWFGTNTDVTALREMEDALREADRRKDEFLA
                     MLAHELRNPLAPLRNGLEVQRLIPGLPDAAHAAREMMARQLDQMVRIVDDLFDVSRIS
                     RGRLELKREPVDLNDVVRGAVETSRPLIDTAGHRLTVTIPPSPVMIDADATRIAQVVA
                     NLLNNAARYTPKAGRIDLSVSRDGTNAIIRVTDSGVGIAAGMLPRVFDMFTQIDRDYT
                     ESTGGLGIGLTLVKRLVEMHGGWVQAASDGPGKGAEFVVRLPLATVVSPSRSVPGATA
                     GSAGGSGNGRTVLLADDNADAARSLAMLLTLKGHKVHTVGDGQAAVERAAEIMPDVVI
                     LDIGMPRMDGHEACRQIRAAASDRPPLLVALTGWGQQEDRNRSEAAGFDVHLVKPVDP
                     SELERLMLDGNARRRSERFLPGPRADTSGGPPTKSVD"
BASE COUNT         1499 a         2945 c         3129 g         1551 t
ORIGIN      
        1 ctacaccggc atgggcgggc cgcttttcct gcgcgcctac gccttcgatg tcgacgaaac
       61 gaccggctac atcctgggtg gctcccgcgg cagggaagag gcgcccgacg aggggaagtt
      121 cacgctcacg ctcacccgtg aagcgggcgg cccctggctc atcatgtccg acatggacaa
      181 ccacaacacc cgatgatcat cggaatcctc ggcgcgggcg cggtcggcgg ctactacgga
      241 gccatgtggc accgggccgg tcacaacgtg cgactgctcg cgcgaggtgc gcacggagcg
      301 gccatcgccc ggtcgggtct ttccgtgaag agcagcttcg gggaagtgac aattccaccc
      361 agccagctcg tcaccgacat cgcccgggaa acaccggtcg atgtcctcgt cgtctgcgtc
      421 aagctgcgcg acacggaatc ggcctgccgt tcggtggcgc catgggtcgg tcctcgcacg
      481 atcgtgatgt cgctccagaa cggagtcgac aaggacgaca tgctcgacaa cgtcttcggc
      541 ccggagcggg tggcgggggc ggtcacgtat atcgtggcca cgatcgagga gccgggggtc
      601 gtcgggcaca agggtaatat cgcccggatc gtggcgggcg tccgtacatc cgggacggcg
      661 cccgggctgt cgctcgcgct cgattccatg cgcgtgcccg gcgtggaagt cgtcgcggcg
      721 gaagatgtgc gccaggaaac ctggcgcaag ttcatcttcc tctcgacatt gagcggcatg
      781 accgccgtta cggggcgccc gatcggcgag ctgcgggatg atcccgaaac ccgtcagcgc
      841 ctcggcgaca tggttgcgga gggggcgaac gtggccgccg ccgaaggaat cgagttcgat
      901 caggaattcg tcgaggactc gatggaattc atcgacaagc tccccgctga cggccgctcg
      961 tccatggcgg tggatctttc gcgtggacag cctctcgagc tcccctggct gagcggggcg
     1021 atggcccgcc gtgcggagcg cctcggcgtt cccgcgccga cgcaccgccg gatcgccgaa
     1081 gttctcgcgc cctacgcgga cggcggcggc gatcgatatc gacccgccgc ggacctctgg
     1141 tcgtaacggc ccgccgatgg tccgctagaa tactcattcc aattcgtcct gcgacatcgg
     1201 ctgtcgatga gccgactcat cgaacggcgc gatcacacgg agctctccca tgtgccggaa
     1261 catcaagaca ctcttcaact tcgacccacc cgccaccgac gccgagatcc atgacgcctc
     1321 gctccagttt gtccgcaagc tgagcggctt caccgcaccg tcgaaggcaa acgaagcggc
     1381 gttcaaccaa gccgtcgagg agacggcccg cgtggcgcgc cggctcatcg acgcactcgt
     1441 caccaacgcc gagccgaaga accgggacga ggaagccgcg aaagccaagg cgcgagcggc
     1501 cgtccggttc ggaagcaact gaggtaatca tggctgaata tcgtgccgtc accgagtgga
     1561 ggtgcggcga ccaaccgttc atcgacaacc gctacagccg ggcccatcgc tggacgttcg
     1621 acgagggagt gagcgtgacg gcgtccgcct cgccgttgca cgtgcccctt cccttgtcgg
     1681 tgggggcggc cgtcgatccc gaggaagctt tcgtggccag cctgtcgagc tgtcatctcc
     1741 tcacgtacct gtacctggcc gcgaagaagg gttttcgcgt cgagagctac cgggacgagg
     1801 cggtggggcg gatggagaaa ggggaggatg gcgggctctg gatgagcgtc gtcacgcttc
     1861 ggccggtcgt cacgtttggc ggtgacaggc ggccgtcagc cgcggaagcc gaggcactcc
     1921 atcacgacgc gcacgccgag tgctacatcg cgcgatcggt gaggagcgag gtgcgctgcg
     1981 aggccacgtc actgggggtg gcatgaagga cctggcgatc cggctcgaca acagtcctgg
     2041 agccctggcc gccatgggtg aggcgctggg cagggccggc gtgagcatcg aggggggcgg
     2101 cgcctgggtg gtggacggcg tcggcgtggc ccacttcctc ttcgaggatg gggccgccgc
     2161 caggcgcgcg ctggaagccg ccggcatcac ggtgctggcc gagcgggagg tggtggtgca
     2221 gcgcctcaag caggccgtac cggggcagct cgggcagctg acacgtcgta tggctgacgc
     2281 cggcgtcaac atcgaggtgc tctacagcga tcatgaccac cagctgatcc tcgtcgtgga
     2341 cgacgtgacg acgggccgca cggtggccga ggcctggagc cgggaagggt aggctccgac
     2401 tccaggcccg tggatcagct cgagtacttg atcacctgga acaccacccc ctgtggtgac
     2461 ttgagcaccg aggcccgccc cactcccggc acgtcatgcg gcggcatgat gaccgtgccc
     2521 cccagttcgg ccgtcagcgc cgcagccttg tcggcgtcgt cgatcgtgaa gaccgtgctc
     2581 cagtggggct tcgtgccgcc cgattccggc atcaggatca tgcaaccggc caccggcgcg
     2641 ccgtcgagcg caaagaccgt gtattcgaag ccgggcatgg acatgacccg cgtcgtccag
     2701 ccaaagagtt cggtatagaa cgacacgcag gccgccgggt cggtggtgag gcactcgaac
     2761 cagctggcgg cgccgtggtg cgtggagtcg tcggtgcggg tggcctgttg tccctcctgc
     2821 cacacatcga agttggcgcc cgatggatcg aagcagaccg ccatccggcc gttggggccc
     2881 acatcgaagg gggcctgggc cttgcctccc agcgcgttga cccgcgcagc cgtggcgtcg
     2941 acgtcggcca ccttgatgag gacaccgatg tggggcggcg cgccctgcat cgagaactgc
     3001 ccctcggtgt cgaacatggc ccccatgttg tggccgtcca ccttgaggag gtggcccatg
     3061 ccggggatct cgccgtagct ccagccgagc accttggtga agaactcctg tgccctggcc
     3121 ggctggggcg tgagcatgtt cgtccagcag aaatcgtgga acttgcgtgg tccggtcgtc
     3181 atgacttctt tcctttcgtg gcggcgagac gcttccgtac cgccggtttc atgagatcgc
     3241 gcagggcgtc cttgccgacc cagcgggccg tcgggttgtc cgacgccgcc agtttctcgg
     3301 cgaggctgat ggaagccgcg tacacggccg ggctgcgccg tccgacgagg cgcaacgccc
     3361 agctcacccc tttcttcacg aagttgcgtt catcggtggc acccgcctcg atgatggcaa
     3421 gggatgccag aaagggggca tcgcccgacg ccttgtcgtg cgctgcgagg caggccagca
     3481 acgcaaaggc accccgcttc tggaactcgt ccttccgccg gctccacggc cccaccacct
     3541 tccaggcgag cggggagagg tcgaggaaat tgaagcagag cgtgtcgacc accccccagt
     3601 tgtcgaaatc cttcgaccag gcgttcatct gcgccacggt cacgcgggcc ggatcgccta
     3661 cgaacgccgt gaggagttgc gcctcgtaca caccggtctt ccagagggcc agcgcaagcc
     3721 cgtgatcgcg ccccagctgc tttcccagtt gcttgatcgt gccgacggga acaccgtaag
     3781 cgttctttgc cgtgatgccg tagcgcgcca tcccatcacg ggtcctcttc gttccccgct
     3841 tctcgagcca ggcgagcgcg tcggcaaccc gttccttcgt ggtcacgccg ccgccaggag
     3901 ggtatcgaga cgggcgaagc ccatgtccat cccttccttc attcccgtct tgagcgcggc
     3961 gtcacgagtc tccttgctgg cgaagtgcat ggtgagcgtg gccgtggtgt accccgccga
     4021 ctcggtaaag accaccgtgt tcagcgtctc cggccattcg ggtccccacg actcggtgga
     4081 aacgacgcga tccggcgggc tgacctcgcg gaagaagccc gacatcgaca tctcggcgcc
     4141 gtcgtccttg cgccacacgt aacgccaggc gccacccggc ttcaggtcga cttcgcatac
     4201 cggcatcgtc cagccgtccg ggccggtcat ccagttgggg agatgcttcg ggctggtcca
     4261 ggcgtcgaac accagggcgc gcggcgcctt gaggacgcgc gtgaccacga tgtcggtgtc
     4321 gttgggcgtg gcatagtcgg tcacgccggg tttaagtacg gtcatcatgg tcggtctcct
     4381 tgtccggttc acgttgcagt tgatcgaggt aggtgtcgag gttccggtag ctcgcatccc
     4441 agaagcggcg gaattccccc gtccactccg ccacttcctt caacggggcg gcttcgaggg
     4501 tgcggggccg ccattgggcc ttgagcccgc gctggatgag cccggccttc tccaggactt
     4561 tcaggtgctt ggagacggcc gggccggtca tgtcgaacgg cgccgacagc tccttcaccg
     4621 tggccgatcc cctggccagg cgcaccagga tggcccgccg ggtggggtcg gccagggccg
     4681 aaagggtgag gctgagggtg tcggatgccg cgtgcatcgt gttgtggccc tttgtgaaaa
     4741 gccgaatggt taaataacta atcggtaatg taaacaggac agccgaccgg gtcaacttct
     4801 tttttctggc cgccgttcgg ggcgttcctc atgcgcgtgt gccaacccgg atgtcattct
     4861 cattcaatga gtccggagta cgacgtcatc atcgtggggg ccggcaccgc cggcttgagc
     4921 gcggcgctca ttctcggcag ggcgcggcga cgggtccgcc tgctcgactg ggggtcgccg
     4981 cgcaatgcgc cggcccggac cagcaatggg gtgttcacgc gcgatggcac accgccggat
     5041 gagctggcac gactggcctg cgcgcagctg gcgccatacg agaccgtttc cgtgacgcgt
     5101 gtgccggccc tgggcgccag ggagctggat ggcgggttcg aggtgaacct cgaggggggc
     5161 caggtcatca cctgccggaa gcttctgctg gcgcacggcg tagtggatga gatgccggcc
     5221 ataccgggag tgcccgagct gtggggttca gcggtcttcc actgcccgta ctgcgacggt
     5281 tgggaagtga gggaccagcc gctggccttt catcccaacg gtgacggcga ggcggccctc
     5341 aacatggcca ggcttctcct ctgctggagc cgcgacctcg tcctctgcac gaacggccag
     5401 accctcctgt cgaaggacga tcggcggatg ctggagcgct ggggagtggg cgtccgcgag
     5461 gagccaatgg tccggatcga ggcgtgtgac accgaggcag ctcagatcgt cttccaggga
     5521 ggaggggcgc ttgcgcgccg ggcgctggtt ctgcaccccc accagcacct ggcgtgcgac
     5581 atggccctga ccctcgggtg caccctggac gacaaggggc ttctctccgt cgacccgcga
     5641 ggggagaccg ccgtgccggg ggtatacgcg gccggtgaca tcatcaccgg aattcagatg
     5701 gtgatccggg cggcggcttc cggcgccacg gcggcggtgg ggatcaataa cgcgctggcc
     5761 ctggacgttt gaagcccggg tgcagcggcg tcggacagac tttatcattg tggtgattgc
     5821 catcgtcatc gtcctttccc cgagtgtgga ggtctcatgc cctacgtcga cggtttcgtg
     5881 cttccggtgc cggtgaagaa cctcgaggcc taccgccgga tgtcgcgcaa ggccggcaag
     5941 gtgtggatgg agcacggcgc gctggaatac cgcgaatgtg ccggggacga tctcaacctg
     6001 aaaatggccg tcaactttcc gaagatggcg aaggtgaaga agggtgagac ggtcgtcttc
     6061 tcgtggatcg tctacaagtc gaaggcgcat cgcgatcgcg tcaacaagaa ggtgatggtt
     6121 gaccccagga tcacctccat cgaccccagg acaatgccct tcgactggaa acgaatgatg
     6181 tacggcggat tcaagaccat cgtggacatg tgagggcggt cgcatcatga gcgatccgaa
     6241 cgactttccc tatgaaacgc acctcgacat taggtatccc cccctggcgg tggtcgacgt
     6301 gcccgccctc gaggcggccg tcgcgcaccc ctggtacaac cagacgctct gccgcgtgaa
     6361 cggctcggtc gtgcgcctcg gcgtgatgca gggggagtac cactggcaca agcacgacga
     6421 cgatgacgag ttcttcttcg tgctcgaggg acgcttcctg atcgacctcg aggaagagac
     6481 gatcgacctc gggccgaagc agggttacgt ggtgccgaag ggcgtggtgc atcgcacgcg
     6541 ggcaccggaa cgggcggtta tcctcatggt tgagaacgcc ggcatcattc ccaccggaga
     6601 ttgaacgtga tcttccgcga gaagcccggt gcgatcctgg cttacgggat caccgtggcc
     6661 gccgttctgg cggccgcgat cggccgtctc ctgatcgacc cggtggtggg agggagcgtt
     6721 cccttcatca cgctcttccc ggccgtcgtg gtggcggcct ggatcggcgg gttcggcccg
     6781 gccttcctgg ccaccgtgct gggcttcgtc ttctccctga tctatttctc gcctccccgg
     6841 ttccagttcc acgatgcctc ccgcgcgcag gaggtgagcc tcacgttgta cgtgctggtg
     6901 agcctcacca tcgccggctt cggcgaggcc atgtggcggg cgcgccggcg gtcacaggac
     6961 gcggcggcgg agcagctccg gagcagccac ctgttgcgca tcaccctggc ctcgatcggc
     7021 gacgcggtcg tcaccacgga tgacgcgggc cgggtgacct acctgaacca cgtggcagag
     7081 gagatgaccg gctggacggt agacgaggcg gtcggcaggc cgcttccgga agtgtttccg
     7141 atactggacg aggcgacgca ggtgtcggcg gagagcctgg tgggccgggt cctccggggt
     7201 ggaaatgccg tcccgctttc cgggcagagc ctgctggctc ggaaggacgg cacgatgcgc
     7261 cccgtggagg agagtgcggc ccccattcgc aacgacgatg gctcggtggt gggcgtggtt
     7321 ctggttttcc atgacatcgc cgaacgacgc gccgccgaga aggcgctgcg cgaccgggag
     7381 cagcgcttcc gtgacatggc caaccacatc acgccgatcg tctggacgtg cgatgccagc
     7441 ggccgcgcca cctgggtcaa tgagcgctgg tacgaatatt ccgggacgac cttcgatgag
     7501 atgacgggcg tcaccgggaa ggacatcctc catcccgacc accgggaacg ggttcgcggt
     7561 gggctcagga acgcgttctc cgagggtcgg cactgggaag acacctttcc actcctgggt
     7621 cgcgacgatc ggtatcgctg gtttctctcc cgcgccgtgc cgatccgcga tgacgaaggg
     7681 cgcgtgctgc agtggttcgg cacgaatacc gacgtgaccg cgctccggga gatggaggat
     7741 gcacttcgtg aggccgatcg ccgcaaggac gagttcctgg cgatgctggc ccacgagctt
     7801 cgaaacccgc tggcaccgct gcgcaacggt cttgaggtcc agcgcctcat tccaggtctt
     7861 ccggacgccg cccacgccgc ccgcgagatg atggcgcggc agctggacca gatggtgcgg
     7921 atcgtggacg atctcttcga cgtcagccgc atcagccgcg gccgcctgga gctcaagcgc
     7981 gagccggtcg acttgaacga cgtggtgcgg ggcgcggtgg aaaccagccg gccgctcatc
     8041 gatacggccg gtcatcgact gacggtcacg attcccccga gcccggtgat gatcgacgcg
     8101 gatgcgacgc gcatcgccca ggtcgtggcc aacctcctca acaatgccgc acggtacacg
     8161 ccgaaggcgg gacgcatcga cctgtcggtc agccgggacg gtacgaacgc gatcatccgc
     8221 gtgacggaca gcggagtggg gatcgcggcc gggatgctcc cgcgggtctt cgacatgttc
     8281 acccagatcg atcgtgacta taccgagtcg accggcggcc tcggcatcgg actcacgctg
     8341 gtgaagaggc tggtggagat gcacggcgga tgggtgcagg ccgcgagcga cgggcccgga
     8401 aagggagccg aattcgtggt tcggcttccg ctggccacgg tggtatcccc ctcgaggtcc
     8461 gtcccgggcg cgacggcggg atcggcgggt ggcagtggca acgggcgcac cgtccttttg
     8521 gccgacgaca acgccgatgc ggcccgtagc ctggccatgc tcctgaccct gaaggggcac
     8581 aaggtgcata ccgtcggcga cggacaggcg gcggtcgagc gggccgccga aatcatgccc
     8641 gacgtggtca tcctcgacat cggcatgccc cgcatggacg ggcatgaggc gtgccggcag
     8701 atccgggccg ccgcctccga tcgcccgccg ctcctcgtgg cgctgaccgg atgggggcag
     8761 caggaagatc gcaatcgttc ggaggccgcc gggttcgacg tccatctcgt gaagccggtc
     8821 gatccgagcg aactggagag gctgatgctc gatggcaacg cgcggcggcg cagtgaacgg
     8881 ttccttccgg gtccccgggc ggatacgtcc ggggggccgc cgacgaagtc ggtcgactga
     8941 tataatccgg agccgatgac gcacacgtcg cttccctccc gatccggccg cccgatgccg
     9001 cgaagctcga ccatcctcgt cacctgggcc gtgtccgctc tcctcctgct tgccgccgac
     9061 gcccggttcg cgcgcggcga gccgctgatc aaggggcgta cgatcgcgaa gaactccacg
     9121 ccgt
//
LOCUS       DASVOD010000653         5438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_877496, whole genome shotgun sequence.
ACCESSION   DASVOD010000653 DASVOD010000000
VERSION     DASVOD010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877496"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1163
                     /gene="rfbB"
                     /locus_tag="VF720_14070"
     CDS             111..1163
                     /gene="rfbB"
                     /locus_tag="VF720_14070"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007083382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX7880536.1"
                     /translation="MTDRAARGNARKPISLSGATVLVTGAAGFIGSNFVQMLAEGHDD
                     VKIIVLDKLTYAGNPANLAPLEGRKGYRFVKGDIADSAIVDPLVNEADFVVNFAAESF
                     VDRAIEDARPFLRANVEGPLVLLDAARRHGVTRFLQVSTDEVYGEVFGPSATEEFPLK
                     PRNPYAASKAGGDALVFSFGATYDVPVVITRCSNNYGPYQYPEKLIPLFVTNAMDDKQ
                     LPVYGSGMNTRDWIHVSDHCRAIEAVLTHPGAEGEIFNVAAENELNVLEITTMILEAL
                     GKPKTLMNYVADRPGHDRRYALDAGKLRAATGWEPKVTFPEGLHATVQWFLANEAWWR
                     PIKSGEWREYYAKSQA"
     gene            1183..2283
                     /gene="wecB"
                     /locus_tag="VF720_14075"
     CDS             1183..2283
                     /gene="wecB"
                     /locus_tag="VF720_14075"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEX7880537.1"
                     /translation="MKIDCVVGARPNFMKMAPIIEALRLTPGFEPRLIHTEQHYDAAM
                     SKVFFDELGMPVPDLFLGVGSAGHSEQTARVMIEFDKVVAADRPDLVLVVGDVNSTLA
                     AALVAAKRLIPVGHVEAGLRSFDRTMPEEINRILTDVVSDLLFTTSEDAHENLAAEGI
                     DTAKIRFVGNTMIDSLLKHRERARQTSKALATFRLEPGRYLLVTLHRPANVDEPAALS
                     GLLGALGRLSDERPVLFPVHPRTRARFKEFGLEETIARHPGLKFTDPLGYLDFLALME
                     SAELVLTDSGGIQEETTVLGVPCLTLRPNTERPVTISEGTNLLVGTDPERIVAEGLKV
                     LSGDRKSGRAPRYWDGQAAGRIVAALAERGRA"
     gene            2280..3866
                     /locus_tag="VF720_14080"
     CDS             2280..3866
                     /locus_tag="VF720_14080"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX7880538.1"
                     /translation="MRAPFLKRAHAPALLSILALVMSVPAPAQSIDESLLPPSIREAL
                     NASPTTPVAEPNLPLTLGDGPVDPALYHLGPGDELTIRYRGRTTATHRLLVGPEGAVY
                     LPDVGRIDLSGRTLSEGREALMTAARKILRDVTIEVDLTRVRSFKMSVTGQVARPGVY
                     AATGSTRVFEILRMAGGVTDSAAVRDLRLVDLRTGESKPVDLLPFLLRGGPPACNPYV
                     PDGATLVVPRRTRWVRLGGAVLQPGAYDLPADGTTVGDLLGLVGLEPDAVTDRIELGR
                     LGEAPKGGTLADLAGTPLVHGDVVTVPRKGTFRRVDLVNVEGEVGFPGSYAVSNDTTR
                     LADFLERAGGLLPGAQTSRILLMRRSLADSLPPAARRLVPYPTVILTLSEKEQLRARG
                     EDGFRAVVIDLAQGEDRGPVLEPGDRILVPRRGAFVEVGGRVRRPGFYAWRAGATAED
                     YVADAGGWADRADKKQTRVTTGPGDNFRTGKDVGSLEPGDHVWVPEKIPRSGWENLRD
                     AVVIVGQLATVVLLIDQLND"
     gene            3871..5037
                     /locus_tag="VF720_14085"
     CDS             3871..5037
                     /locus_tag="VF720_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880539.1"
                     /translation="MSRKRVLHVVTRLAMGGAQKSTVDTCTRLPKERWEVEVLAGPET
                     GEEGSLQEAFTQAGIPVHLLPGLHREEHIGNDLSALWRLIGFLRRGKYDVVHTHMAKA
                     GIIGRMAAGYAHVPAIVHTAHGWPWHNFIDRKTKERYVGHERRAAARTGKILVTSERD
                     RGKALAWQIAPVDRFEMVRTGIDFDIFDPARWERDVARRAHGIPADAPVIISVGALTH
                     QKNPLEALEVVARVRHAFPALHYILVGDGPLRGEVQRRADALGLGDSFHPLSVRHDVP
                     SLLAAADVFLLTSRWEGLPRTLIEAMAMGKAPVVTQVDGVLDVIEDNVTGYVRDPGDV
                     DELTAMIVRLFRAPNLIKEMGIQNPKFIRKPEFSVERMVTRIGDVYDRLLAAPR"
     gene            5034..>5438
                     /locus_tag="VF720_14090"
     CDS             5034..>5438
                     /locus_tag="VF720_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880540.1"
                     /translation="MKPARLLLVSAVLVVALHWIGAFLPGPNTWGFHHWAFLPRPLLI
                     LVGLLATALLLPPVAGTMAGLLAPLARSIDRGRRAVAPVAVIVVLFGVAFWLGRQRLF
                     LLSDGELLVRMVARGVPSLTFTVDTLATLIHLW"
BASE COUNT          847 a         1919 c         1813 g          859 t
ORIGIN      
        1 cgcacatgta cgactacaag gagccggatg aattccgggt gcacccccat gacaacgaca
       61 ttccatacga ctggaaccgc aaggatggct gacgcccggg gaggcctctc gtgaccgatc
      121 gcgccgcgcg cggcaacgcc aggaagccga tctccctttc cggggccacc gtcctcgtga
      181 ccggtgccgc cggcttcatc ggttcgaact tcgtccagat gctggcggag gggcacgacg
      241 acgtgaagat catcgtgctc gacaagctca cctatgccgg caacccggcc aacctggctc
      301 cgctcgaagg gcggaagggg taccgcttcg tgaaggggga catcgccgac agcgcgatcg
      361 tcgatcccct ggtcaacgag gccgatttcg tcgtcaattt cgcggcggag tcgttcgtcg
      421 accgcgccat cgaggatgcc cggccgttcc tgcgcgccaa cgtggaaggg ccgctcgtcc
      481 tgctcgacgc ggcccggcgg catggcgtca cccggtttct ccaggtatcc accgacgagg
      541 tgtacggcga ggtgttcggg ccctcggcca ccgaggagtt tcccttgaag ccccgcaacc
      601 cctacgcggc cagcaaggcc ggcggtgatg cgctcgtctt cagcttcggc gccacctacg
      661 atgtgccggt cgtcatcacc cgctgcagca acaactacgg tccgtaccag tacccggaaa
      721 aactgatccc cctcttcgtg accaacgcca tggacgacaa gcagcttccg gtctacggct
      781 cggggatgaa cacgcgcgac tggatccacg tgtcggacca ctgccgggcc atcgaggcgg
      841 tgcttaccca tcccggggcc gagggggaaa tcttcaacgt ggcggccgag aacgaactga
      901 acgtcctcga gatcaccacc atgatcctcg aagcgctggg gaagccgaag acactgatga
      961 actacgtggc cgaccgtccg ggccacgatc ggcgctacgc gctggatgcc ggcaagctca
     1021 gggccgccac cggctgggag ccgaaagtca ccttccccga ggggctccac gccacggtgc
     1081 agtggttcct ggccaacgag gcatggtggc ggccgatcaa gtccggcgag tggcgcgagt
     1141 actacgccaa gtcccaggcg tgaggcgggc cgccagatcg tcatgaagat cgactgcgtc
     1201 gtcggggcac gtccgaattt catgaagatg gcgccgatca tcgaggcgct gcgcttgacc
     1261 cccggcttcg agccccgcct gatccacacc gaacagcact acgatgccgc catgtcgaag
     1321 gtcttcttcg acgagctcgg catgccggtg cccgacctct tcctcggcgt cggctcggcc
     1381 ggccacagcg aacagaccgc ccgcgtcatg atcgagttcg acaaggtggt ggcggccgac
     1441 cgtcccgacc tggtcctcgt ggtcggcgac gtgaactcca cgctggccgc cgcgctcgtg
     1501 gcggcgaaac ggctgatccc ggtcgggcac gtcgaggccg ggctccgttc cttcgaccgg
     1561 acgatgcccg aggagatcaa ccgcatcctg accgacgtgg tgagcgatct tctcttcacg
     1621 accagcgagg atgcccacga gaacctggcg gccgagggga tcgacaccgc gaagatccgc
     1681 ttcgtgggga atacgatgat cgactcgctc ctcaagcatc gggagcgcgc gcgccagaca
     1741 tcgaaggcgc tggccacgtt ccgcctcgag cccggacgct atcttctcgt cacgctccat
     1801 cgccccgcga atgtcgatga gccggctgcg ctgtcgggat tgctcggcgc gctgggacgc
     1861 ctctcggacg aaaggccggt gctctttccg gtgcacccgc gcacgcgggc gcgcttcaag
     1921 gagttcgggc tcgaggagac aatcgcgcgg cacccgggcc tcaagttcac cgatccactc
     1981 ggctatctcg attttctcgc cctcatggag tcggccgagc tggttctcac cgattccggc
     2041 ggcatccagg aggaaaccac ggtgctcggc gttccctgtc tcacgctccg tcccaacacg
     2101 gaacgccccg tcaccatcag cgaagggacg aacctcctgg tcggcaccga tcccgaacgg
     2161 atcgtggccg aggggctcaa ggttctctcc ggcgatcgga agtcggggcg cgccccgcgc
     2221 tactgggacg gacaggcggc cggccggatc gtggcggcgc tggccgaacg gggtcgcgcg
     2281 tgagggcacc gttcctgaaa cgcgcgcacg cccccgcact cctttccatc ctcgcgctgg
     2341 tgatgtccgt cccggccccg gcccagtcga tcgacgaatc actcctccct ccctcgatcc
     2401 gcgaggcgct caacgcctcg ccgacgacgc cggtggccga gcccaatctt ccgctgacac
     2461 tcggtgacgg ccccgtcgat cccgcgctct accacctggg accgggcgat gaactcacca
     2521 tccgatatcg cggccggacg acggccacgc accggctcct ggtgggaccg gaaggggccg
     2581 tctaccttcc cgacgtcggt cgcatcgacc tgagcggcag gaccctgagc gaggggcgcg
     2641 aggcgctgat gacggcagcc cgcaagatcc ttcgtgacgt gacgatcgag gtcgacctga
     2701 cgcgcgtgcg ttccttcaag atgagcgtca ccggccaggt ggcccggccc ggtgtgtacg
     2761 ccgccaccgg ctccacgcgg gtgttcgaga tcctccgcat ggcgggcggg gtgaccgatt
     2821 cggccgccgt gcgcgacctg cgcctcgtcg accttcgcac cggcgaatcg aaaccggtcg
     2881 acctgctgcc gttcctcctc cggggcgggc cgccggcctg caatccctac gtgccggacg
     2941 gcgccacgct ggtcgtcccg cgccgcaccc gatgggtccg tctcggcggt gccgtgctcc
     3001 agccgggtgc ctacgacctg ccggcggacg gcaccacggt gggggacctg ctcggcctcg
     3061 tggggctcga accggacgcc gtgaccgacc gaatcgagct gggacggctg ggcgaggcac
     3121 cgaagggggg caccctggcc gacctggccg gaacgccgct cgtgcatggt gatgtcgtca
     3181 ccgtcccgcg gaaggggaca ttccgccgcg tcgacctggt gaacgtggaa ggagaggtcg
     3241 ggtttcccgg aagctacgcg gtgtccaacg acacgacgcg gctggccgac ttcctggaac
     3301 gggccggcgg ccttcttccc ggcgcccaga cgagccggat cctcctcatg cgacgctcgc
     3361 tggccgacag ccttccaccg gccgcccgtc gcctggtgcc gtatcccacc gtgatcctca
     3421 ccctgagcga gaaggagcag ctccgggccc gcggcgagga cggattccgt gcggtcgtca
     3481 tcgatcttgc gcagggggag gatcgcggcc cggtgctcga gcccggcgac cggatcctgg
     3541 tgccgcgacg cggcgccttc gtggaagtgg gagggcgcgt ccgtcgtccc ggcttctacg
     3601 cgtggcgcgc gggcgccacc gccgaagact acgtggccga cgccggcggc tgggccgacc
     3661 gggccgacaa gaagcagacg cgcgtcacga ccggccccgg cgacaatttc cgcaccggca
     3721 aggatgtcgg ttcgctcgag ccgggcgacc acgtctgggt gcccgaaaag attccacggt
     3781 cgggctggga aaacctgcgc gacgccgtgg tgatcgtggg gcagcttgct accgtcgtcc
     3841 tcctcatcga ccagttgaac gactagccgg atgtcacgca aacgcgtcct ccacgtcgtc
     3901 acgcgcctgg ccatgggagg cgcgcagaag agcacggtcg acacctgcac ccgcctgccg
     3961 aaagagcggt gggaggtgga agtgctggcc ggccccgaga cgggggaaga gggctcgctg
     4021 caggaggcct tcacgcaggc cggcatcccg gtgcatctcc tccccgggct tcaccgcgag
     4081 gaacacatcg gcaacgacct gtcggccctc tggcgcctca tcggcttcct gcggcgcggc
     4141 aaatacgacg tggtgcacac gcacatggcc aaggcgggga tcatcgggcg catggcggcg
     4201 gggtatgcgc acgtgccggc catcgtccac acggcgcatg gctggccctg gcacaacttc
     4261 atcgaccgga agaccaagga acgctacgtc ggccatgaac ggcgggccgc cgcgaggacc
     4321 gggaagatcc tcgtcaccag cgagcgggat cgcggcaagg cgctggcctg gcagatcgcg
     4381 ccggtcgatc gcttcgagat ggtgcggacc ggcatcgact tcgacatctt cgatccggcc
     4441 cgctgggagc gtgacgtggc gcggcgtgcg cacggcatcc ccgcggatgc cccggtcatc
     4501 atctcggtcg gcgcgctcac tcatcagaag aacccgctcg aggcgctcga ggtggtggca
     4561 cgcgtacgac atgccttccc ggccctccac tacatcctgg tgggggatgg tccactccgc
     4621 ggcgaggtgc agcgccgcgc cgacgcgctc ggcctgggcg actcgtttca cccgctgtcg
     4681 gtgcgccacg acgtgccgtc gctcctggcc gcggccgatg tcttcctcct cacctccagg
     4741 tgggaggggc ttccccggac cctgatcgag gccatggcca tggggaaggc gccggtggtc
     4801 acgcaggtgg acggcgtgct cgatgtcatc gaggacaacg tcaccggcta cgtgcgcgat
     4861 cccggggacg tcgacgagct gaccgccatg atcgtgcgcc tcttccgcgc gcccaatctc
     4921 atcaaggaga tgggtatcca gaacccgaag ttcatccgca agccggagtt cagcgtggag
     4981 cggatggtca cgcggatcgg cgacgtctac gatcgcctcc tggcggcccc gcggtgaagc
     5041 ccgcgcgcct cctcctggtg tcggccgtgc tggtcgtggc cctgcactgg atcggcgcgt
     5101 tcctccccgg gccgaacacc tggggtttcc atcactgggc cttcctcccg cggccgctcc
     5161 tcatcctggt cggcctcctg gccacggcgc tcctcctccc gccggtggcg ggaacgatgg
     5221 ccggcctcct ggcgccgctc gcccgctcaa tcgatcgcgg ccgccgcgcg gtggcgccgg
     5281 tggccgtgat cgtcgtcctc ttcggcgtgg ccttctggct gggacgccag cggctctttc
     5341 tcctgtcgga cggcgagctc ctcgtgcgga tggtggcgcg gggcgttccg agcctcacct
     5401 tcacggtgga cactctggcc acgctcatcc acctttgg
//
LOCUS       DASVOD010000654         2546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_461347, whole genome shotgun sequence.
ACCESSION   DASVOD010000654 DASVOD010000000
VERSION     DASVOD010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461347"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF720_14095"
     CDS             <1..258
                     /locus_tag="VF720_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018649419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7880541.1"
                     /translation="FHLAILYPTRADLGDALKRLIAAGIPLDGAADHGVSEALYLRDP
                     DGNGLELYRDRPPSEWPRNDEGEITMVTLPLDVDKLLRDAG"
     gene            430..1950
                     /locus_tag="VF720_14100"
     CDS             430..1950
                     /locus_tag="VF720_14100"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX7880542.1"
                     /translation="MLATVVLLLLVIATARAADPPAAVPPPPPLEPSAVRALTEPTGH
                     WSGFARFEGDSTDFHLVFTPKEPGRVVMTVTLPATNLFDVPLGRVTADGDRFRASVLS
                     GQFVGRAGSASDPGGRFVGSFAVAGCEVTFDLARDSRPAPAIVPPIAASSSREPAWSF
                     ATGGPIWSAPTVHDGVVYFGGNDGRIHALDAATGRARWQFQTGGPVVAKPTVTGAYLY
                     VASDDGWLYKIEAGSGREVWRCDTHGGAVKRAWPSLTVEGYDSQASAALVADGLVYVG
                     SADGHLYAIDDATGSPRWDFATGGIIRSTPVMAGNNVVVGSHDHFVYAVNARTGTLAW
                     KHDKQDAVVSTAAVVGNQVLIGGRDSLLEALDAATGQPLWKRFYWLSWVESSAVVAGD
                     MAYIGSSDYQKALAINAATGRVRWTFDTDGSAWSTPAVDSSAVYIGAVGTPGYFIDHR
                     GGFFALDRDTGRELWRICFPPIPGSFTNGVASSPAVGGGCVYFGGLDGTFRAVKLR"
     gene            complement(2086..>2546)
                     /locus_tag="VF720_14105"
     CDS             complement(2086..>2546)
                     /locus_tag="VF720_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880543.1"
                     /translation="ESGHDGIDCRSRSMSDLSEQHPVIGRREVRCESRHRMKVDIADC
                     EHFQNDRIATGNPGGADTGVRGALAEMDDPRAPDKQRWSAISEVRLSHIQLGQMREEF
                     DQPGPVPSHERLDVRQKFFVRELQWLIGFESHAMSMTRAVAFSASTNCDC"
BASE COUNT          391 a          879 c          818 g          458 t
ORIGIN      
        1 ttccacctag ccatcctcta tccgacccgc gccgacctcg gggatgccct caagcgcctg
       61 atcgccgcgg gcatcccgct ggatggtgcg gcggatcacg gtgtttcgga agcgctctac
      121 ctgcgcgatc ccgacggcaa cggcctcgag ttgtatcggg accggccgcc gtcggagtgg
      181 ccccggaacg acgaagggga gatcaccatg gtcaccctgc cgctggatgt cgacaaactt
      241 ttacgtgacg cggggtgact tcgtcgccgc acctgtgatg tgatccggtc ggtccccagc
      301 caccctcggc cggagaccgc catgagccgc gtgtcgattg atcgttccgc cgcttcaccc
      361 gctgcatcac ccgcccgtag acgattgtcc tcacggacat cggaaacctg tattgatgtc
      421 agtcaactgt tgctggcaac agttgtcctc ctgctgttgg tcattgcgac cgcccgggcc
      481 gccgatccgc cggccgccgt accaccgccg ccacccctcg agccctccgc cgtccgcgcc
      541 ctcaccgagc ccacgggcca ctggtccggc ttcgcccgct tcgaggggga ttcgaccgac
      601 ttccacctgg tgttcacgcc gaaggaaccc gggcgcgtgg tcatgactgt tacgctgccg
      661 gcgaccaact tgttcgacgt gcccctgggc cgggtgacgg ccgatggcga ccggtttcgt
      721 gcgtcagtcc tgtccgggca attcgtggga cgggccggct ccgcatccga ccccggcggc
      781 cgcttcgtcg gctcgttcgc cgtggcgggt tgcgaggtga ccttcgacct cgcgcgcgat
      841 tcccgaccgg cgccggcaat cgttccgccg attgcagcat cgagcagccg cgaaccggca
      901 tggagtttcg ccacgggcgg gcccatctgg tccgcaccga cggtgcacga tggcgtcgtc
      961 tacttcggcg ggaacgacgg ccgcatccac gcgctcgatg ccgccacggg acgggcgcgc
     1021 tggcagttcc agacgggcgg ccccgtcgta gccaagccga ccgtgaccgg cgcgtatctg
     1081 tatgtggctt ccgacgacgg ttggctctac aagatcgaag caggttccgg ccgcgaggtg
     1141 tggcgatgcg acacgcacgg cggcgcggtg aagcgcgcct ggcccagcct cacggtggag
     1201 ggatacgact cgcaggcctc agcggccctc gtggcagacg gcctcgtgta cgtcggcagc
     1261 gcggacggcc acctctacgc catcgacgac gccaccggct ccccgcgctg ggatttcgcc
     1321 accggcggca tcatccgctc cacgcccgtc atggccggaa acaacgtggt cgtcggcagc
     1381 cacgatcact tcgtctacgc ggtcaacgcg cgcaccggca cgctcgcctg gaaacacgac
     1441 aagcaggacg ccgtcgtctc gaccgccgcc gtcgtcggca accaggtcct gatcggcgga
     1501 cgcgattcgc tcctcgaagc cctcgacgcg gccaccggcc agccgctctg gaaacggttc
     1561 tactggctgt cgtgggtgga gtcgtcggcg gtggtcgccg gcgacatggc gtacatcggg
     1621 tcgtccgact accagaaggc gctggccatc aacgcagcca cgggacgcgt tcgctggacg
     1681 ttcgacaccg acggctcggc gtggtccacc cccgccgtcg actcgagcgc ggtgtacatc
     1741 ggtgcggtgg gaacgccggg ctacttcatc gatcaccggg gcggcttctt cgcgctcgac
     1801 cgcgatacgg ggcgcgaact gtggcggatc tgtttcccac ccataccggg ctcgttcacc
     1861 aacggcgttg cctcctcgcc ggcggtgggc ggcggatgcg tctacttcgg cggactggat
     1921 gggacgttcc gggcggtgaa gctgcgttga gagtcggggc atcgcttccc gtgctcttgg
     1981 ggcgactggc ttcggcagcg cacgacgcag ctccgatagt cgccggagcc tgtattgaaa
     2041 acagtgaact gttgttgaca acagttgtcg catcgcgaat aaagttcagc agtcacagtt
     2101 tgtgcttgcg gagaacgcaa cggctcgtgt catgctcatc gcatgcgatt caaacccaat
     2161 gagccattga agctcacgaa cgaagaactt ctgtcgcacg tcaaggcgct cgtgcgaagg
     2221 gaccgggccg ggctggtcga actcctcacg catctggccg agttggatgt gcgagaggcg
     2281 tacctcgctg atggcgctcc atctctgttt gtctggtgca cgcgggtcat ccatctcggc
     2341 gagggcgccg cgtacgcccg tatccgcgcc gcccgggttg cccgtcgcta tccggtcatt
     2401 ctggaaatgc tcgcaatcgg cgatgtccac cttcatgcgg tggcgcgact cgcacctcac
     2461 ctcacgccgg ccaatcaccg ggtgttgctc cgagaggtcc gacatgctcc gcgagcgaca
     2521 atcgatgcca tcgtggcccg attcct
//
LOCUS       DASVOD010000655         3209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_983686, whole genome shotgun sequence.
ACCESSION   DASVOD010000655 DASVOD010000000
VERSION     DASVOD010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983686"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..2200)
                     /gene="typA"
                     /locus_tag="VF720_14110"
     CDS             complement(341..2200)
                     /gene="typA"
                     /locus_tag="VF720_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX7880544.1"
                     /translation="MIVEHRNRQENFVTVARRDDIRNLAIIAHVDHGKTTLVDALLWQ
                     SGIFRENQEVVERVMDSNDLEREKGITILAKNTAVRYKGTKINIVDTPGHADFGGEVE
                     RTLKMVEGVLLLVDASEGPLPQTRFVLKKALELHLTPIVVINKIDRKDARIAEVVDEV
                     YGLFIDLDAEEGQLDFPILYTVARNGTVRLTPNGPDSRLGPLFDEIIRTIPAPAHDPD
                     EGLQILITNLDYSDFVGRLAIGRIMNGTVKARDAVSLCKLDGSVVNGKLTNLYVFEGL
                     ERKDATEAGPGELVALAGLDEVSIGETVCNIDRAKALPPIHVDEPTVSILFSVNSSPF
                     SGQDGKYVTSRQLRERLYKEILTNVSIRVEDTDSPDSLKVSGRGELQLAILIEMMRRE
                     GYEMAVAKPEVITKTIDGTLHEPMEQLSIDCPEEFIGVVTQKLGPRKGRMTKMTNHGT
                     GRVRLEFRIPSRGLIGFRSEFLTDTRGTGILNHLFDGWEPWQGTITARIAGALVADRP
                     GKTTGYAIEHLQDRGEMFIKPGEKVYEGMIVGENARANDLDVNIVKEKKLTNMRASGS
                     EESIPLLPPRQFSLEQALEFIKDDELLEITPQNFRFRKKVLAANMRPKKYEEK"
     gene            2284..>3209
                     /locus_tag="VF720_14115"
     CDS             2284..>3209
                     /locus_tag="VF720_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7880545.1"
                     /translation="MIVPAALLGLILLSFRTIPADSRGVIEGFGPPRVVGPGRHFVPP
                     LVGSVTAYPSGERTTFVATHEDVGTVAIRYEIPESGLLHFHQAMRGSGRDSLAVIVLE
                     QVRLARAAESAGPSDASRVASLAAVRSRTAVEALGARLIGVTVDGVSRSPAEPPALTS
                     EVAASPARPRPVLLIGIDSADWTIIDRLRKKGYLPALSSMITNGASGPLRSIEPLLSP
                     LIWTTIATGTSPARHGILDFLVTDPATGKQVPATSRLRREPAFWNLVSSAGKSAGVVG
                     WLATWPAESIEGFVVTDRFGFLAFAGQVAPAA"
BASE COUNT          465 a         1051 c         1076 g          617 t
ORIGIN      
        1 ggccgcatct acacgttcga gacgcgcacg aatctgctcg tggtcgaccc ggatgccatc
       61 ctgtacggcg cggaggtccg gatgcagggg ctggcgccgg ccgacacgag ccgcgttgct
      121 gacacgccgc cggggactac cggcgggtaa cgcgggctac ggcgatggag tcaggacgac
      181 atgctcccgg cggatgatct ccctgaaaga ttcgtccgtc gtgcaccgat cgccaacgtc
      241 cacctgccca gactttcacc ggtgaaagta aaaggtggca gcgaaacagg ttcgtcagtc
      301 gcgcggaact gtcaccggta gaaagttccg ccccccgccc ttacttctcc tcgtacttct
      361 tcggccgcat gttcgccgcc agcaccttct tccggaagcg gaagttctgg ggcgtgattt
      421 cgaggagctc gtcgtccttg atgaactcca gcgcctgctc gaggctgaac tgtctgggcg
      481 gcaggagggg gatcgactcc tcggagccgg aggcgcgcat gttggtgagc ttcttttcct
      541 tcacgatgtt cacgtcgagg tcgttggcgc gggcgttctc tccgacgatc attccttcgt
      601 acaccttctc gcccggcttg atgaacatct cgccgcggtc ctggaggtgc tcgatggcgt
      661 agccggtcgt cttgcccggg cggtcggcca ccagcgcgcc ggcgatacgc gcggtgatgg
      721 tcccctgcca cggctcccag ccgtcgaaca ggtggttcag gatgccggtg ccgcgcgtgt
      781 cggtgaggaa ctcggagcgg aagccgatga ggccgcggct cgggatgcgg aactcgaggc
      841 gcacgcggcc ggtgccgtgg ttggtcatct tggtcatgcg ccccttgcgc ggccccagct
      901 tctgggtgac cacgccgatg aactcctcgg gacagtcgat gctgagctgc tccatcggct
      961 cgtggagcgt gccgtcgatc gtcttggtga tgacttccgg cttggccacg gccatctcgt
     1021 acccttcgcg ccgcatcatc tcgatgagga tggccagctg gagctcgccg cgtccggaca
     1081 ctttgagcga gtcggggctg tcggtgtctt ccacgcggat cgacacgttg gtgaggattt
     1141 ccttgtagag gcgctcgcgg agctgccggc tggtgacgta tttgccgtcc tggcccgaga
     1201 agggggagct gttgaccgag aagaggatcg acaccgtcgg ctcgtccacg tggatcggcg
     1261 gcagcgcctt ggcacggtcg atattgcaca ccgtctcgcc gatcgacacc tcgtcgaggc
     1321 ccgccagtgc caccagctcg ccggggccgg cctcggtggc atccttccgc tcgagcccct
     1381 cgaagacgta caggttggtg agcttcccgt tcaccacgct gccgtcgagc ttgcagaggc
     1441 tcacggcatc gcgcgccttg acggtgccgt tcatgatgcg cccgatggcc agccgcccca
     1501 cgaagtccga gtagtcgagg ttggtgatca ggatctggag cccctcgtcc ggatcgtgcg
     1561 ccggtgcggg gatcgtcctg atgatctcgt cgaacaaggg tcccaggcgg gagtccgggc
     1621 cgttgggcgt gaggcgcacc gtgccgttac gggccaccgt gtaaaggatc ggaaagtcga
     1681 gctgcccctc ctcggcatcg aggtcgatga agaggccgta cacctcgtcc accacctcgg
     1741 cgatccgggc gtccttccgg tcgatcttgt tgatgacgac gatcggcgtg aggtgcagtt
     1801 ccagcgcctt cttgaggacg aagcgggtct gcggcagcgg cccttccgag gcatccacca
     1861 ggaggagcac gccctccacc atcttgagcg ttcgctcgac ctcgccgccg aagtcggcgt
     1921 ggccgggggt atcgacgatg ttgatcttcg ttcccttgta gcgcaccgcc gtgttcttgg
     1981 ccaggatggt aatccccttc tcacgctcca ggtcgttgga gtccatgacg cgctccacga
     2041 cctcctggtt ctcgcggaaa atgccgctct gccagaggag ggcgtcgacc agggtggtct
     2101 tgccatggtc gacgtgggcg ataatggcca gattacggat gtcgtctcgg cgggcaacgg
     2161 tcacgaagtt ctcctgacgg tttcggtgtt cgactatcat ggtgaaatga agcgggacag
     2221 tgtatcagat gcggctcccc cccgggggat gatttgaggc tatccggccg cggcgtcctg
     2281 ctcatgatcg ttccggcggc ccttctcggg ctgatccttc tctcgtttcg gaccatcccc
     2341 gcggattcgc gcggcgtcat cgagggattt ggcccgcccc gggtggtcgg tccgggccgc
     2401 catttcgtgc ctccgctcgt ggggagcgtg accgcctatc cgtcgggaga gcggacgacg
     2461 tttgttgcca cccatgaaga cgtggggacg gtcgcgatcc gctacgaaat tcccgaatcg
     2521 ggccttctcc atttccacca ggcgatgcgc ggcagcggac gcgactcgct tgccgtcatc
     2581 gtcctggagc aggtccgcct ggcccgggcc gccgagagtg ccggcccatc agacgcctcg
     2641 cgtgttgcat cgctcgccgc cgtgcgcagc cggacggctg tcgaggcact gggcgcccgg
     2701 ctcatcggcg tgaccgtcga cggcgtgtcg aggagcccgg ccgaaccgcc ggcgttgacg
     2761 tcggaggtcg ccgcatcccc cgcccgccca cgtcccgtcc tcctcatcgg catcgactcc
     2821 gccgactgga cgatcatcga ccgccttcgg aagaagggtt acctccccgc gctctcgtcc
     2881 atgatcacga acggcgcgtc aggcccgctc cgctcgatcg agccgctgtt gtcgcccctc
     2941 atctggacga cgatcgccac gggcacctcg ccggcgcggc acgggatcct cgacttcctc
     3001 gtcaccgacc cggccacggg caagcaggtc ccggccacga gccgcctgcg gcgtgagccg
     3061 gccttctgga acctggtgtc gagtgccgga aagagcgcgg gcgtggtcgg gtggctggcc
     3121 acctggccgg cggagtcgat cgaggggttc gttgtgacgg accggttcgg gttcctggcg
     3181 ttcgcgggac aggtggcgcc ggcggcgga
//
LOCUS       DASVOD010000656         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_877752, whole genome shotgun sequence.
ACCESSION   DASVOD010000656 DASVOD010000000
VERSION     DASVOD010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877752"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..1043
                     /locus_tag="VF720_14120"
     CDS             156..1043
                     /locus_tag="VF720_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880546.1"
                     /translation="MTSARSGGSRPNGPVPAMLLSSALVFLCLAGPATPLLAQDARNA
                     DEMLRNGIANLEAQRNRLLPGIKDATTTLAAIREVHDQRRVAHRVIALDYPLDDFAGI
                     VMADRYDDTYLAVIDSLAFRFGQLDRDLPSEEGANLLREGVGTLPPVTETSSPFVIAY
                     IIGKVLPGAAALPADTPPQLRADAAESAVRMIGALTTIYRRSAAKRESADSWHESVVE
                     RLRCPTHKSSYRIEELRNGLRADGSLYRRYIVTCESGKEQRRLDFDLGAMGALSQNKG
                     RQNLKQKIPPAGSRQPGVN"
     gene            1226..>1505
                     /locus_tag="VF720_14125"
     CDS             1226..>1505
                     /locus_tag="VF720_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880547.1"
                     /translation="MGRRYGIPFMALALGLLSAPVVAMAGVPDPALSTIPNVVGAPNG
                     QMAYKVTIVGSSGPIDSANVTLVWSTAGDTAACWCTSQNHPSVSASTNP"
BASE COUNT          277 a          521 c          467 g          240 t
ORIGIN      
        1 gttcgtgtcg acaggtcgac gcgaacaggg caagattgta tcgtcgaaaa cccgcatggg
       61 tcaaggaaat ggcggcagat cggcctcccg gggccctgcg ccgccccgtt tttcacgcga
      121 ggacgaatca gttgatacct cgattgaatg ccatcgtgac aagtgctcga tccgggggat
      181 cccgccccaa cgggcccgtc ccggccatgc tcctttcctc ggcgctggtc ttcctgtgcc
      241 tggccgggcc ggccaccccc ctcctggccc aggacgccag gaacgccgac gagatgctgc
      301 ggaacggaat cgccaacctg gaagcccagc ggaaccggct gcttccgggc atcaaggacg
      361 ccaccaccac cctcgccgcc atccgggaag tgcatgatca gcgccgcgtg gcgcaccgcg
      421 tcatcgccct cgactacccc cttgatgact ttgccgggat cgtcatggcc gatcgctacg
      481 atgacacgta cctcgccgta atcgattccc tggccttcag gttcggccag ctcgaccggg
      541 atctcccgtc ggaagaaggc gccaacctcc ttcgcgaggg tgtggggacc ctgccgccgg
      601 tcacggagac gtcatccccg ttcgtcatcg cctacatcat cggaaaggtc cttccgggcg
      661 ccgcggcgct gccggcggat acgccccccc agctccgggc cgacgccgcc gaatcggccg
      721 tccggatgat cggcgccctg acgaccatct accggcgaag tgccgcgaaa cgcgaaagcg
      781 cggacagctg gcacgaatcc gtcgtggagc gcctgcgctg tccgacccac aaatcgtcct
      841 acaggatcga ggagctccgg aacggacttc gggccgacgg cagcctctat cgccgctaca
      901 tcgtgacctg cgaatccggg aaggagcagc gccggctcga cttcgacctg ggggcgatgg
      961 gggcgctgag ccagaacaag gggcggcaga acctcaagca gaagattcct cccgccggct
     1021 cgcgccaacc cggggtcaac tgacccggag gggccggctg gccgccctac ccgcggccgg
     1081 ggggaagccc ggggccgaaa accctgcaat ttcaatcttg aaggggaacg aggcgtccga
     1141 taaactcccc tgtgcctgca atccaccgag aaacaggccg tccaacaacg atttgaccag
     1201 cccgggacat tcaggaggtg catcgatggg tcggcgttac ggcattcctt tcatggcgct
     1261 tgcgttggga cttctttcgg ctccggtcgt ggcgatggcc ggtgtgccgg atcccgcact
     1321 cagcacgatt ccgaacgtcg tcggcgcgcc caacggccag atggcataca aggtaacgat
     1381 cgtcggctcg tccggaccga tcgacagcgc caacgtcacg ctggtctggt cgacagccgg
     1441 tgataccgcg gcctgctggt gcacatcgca gaatcacccg agcgtatccg cgtcgacgaa
     1501 tccga
//
LOCUS       DASVOD010000657         8697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_461487, whole genome shotgun sequence.
ACCESSION   DASVOD010000657 DASVOD010000000
VERSION     DASVOD010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461487"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="VF720_14130"
     CDS             complement(<1..1053)
                     /locus_tag="VF720_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020561392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HEX7880548.1"
                     /translation="MASRKSRKPVVPSRRASDAPSRVLFRARAPLRISFCGGGTDVDP
                     YPAEKGGAVLSTTIDKFAWATLRPRRDVRMSIHSLDYNLVANYHMKGKKLAYDGELDI
                     VKAVANHFQPKSGFDLFLHSDAPPGSGLGSSSTMTVALVGLMREWLRQPLTDYEIAEL
                     TYLIERVELKLAGGRQDQYAATFGGFNFIEFHDGVTVVNPLRIKPATVRELEYNLLLC
                     FTGRTRESAGIVAEQTRAYVDRKKPVVRALDRMKSLTIEMKSCLLRDRLADFGSLLHT
                     AWQEKTRLSDRISNERIDDLYAEARKAGALGGKILGAGGGGYLLIYVPFDSRHKVAGK
                     LEAGGGQVVPFSFREDG"
     gene            complement(1031..1774)
                     /locus_tag="VF720_14135"
     CDS             complement(1031..1774)
                     /locus_tag="VF720_14135"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7880549.1"
                     /translation="MGFQRQVQNLTTRQAVILAGGLGTRIASLSGGLPKALLPIAGRP
                     FLHHLLEYLAGQGISRALLLLGVGADKVIAAANDVSARNLAITTAVEPEPLGTGGALR
                     HALALLDDRFFLVNGDTFLRTRLSEIDVRHLSAPVPVATLALTRFADAGQKGSVELNE
                     EGFITRFLEKGKVGPGLINAGVYLIEKGGVRDIPAGRPVSLERDILPAWINRGGHASL
                     RGVVTDAYFVDIGLPEDYLGVIDGFPKES"
     gene            2134..3291
                     /locus_tag="VF720_14140"
     CDS             2134..3291
                     /locus_tag="VF720_14140"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX7880550.1"
                     /translation="MSHRDRVLPTVTAAAVALFLASAAQAGLPRIASEKVPQSDEVKW
                     DVMETFDLNATLAEIEPNDTQAQAQPMSCGDVINPAYLEAGGFDVYRFTATAGQLLTL
                     GTDSNGGLDTDTWIYLIDAAFTTALAQDDDSGPGAYSLISGFAAPYTGTYYFAVEGYD
                     VEVDEGDYRGFVNCSSTPPPTNCSITDYKGQVVTLPTPVAIPDNNLAGVSVGTINTPN
                     DLTKFLDVIVSLKVTHTWVGDLVATLTYDETCDGQPEASSRFICRPGRATCDNVLGTP
                     FGCSSNLACANNMFFSDGATNVLGTSPTGCGTSSTVLAAGCWKGGSNAQPLSVFDNFR
                     KGGCFKLNVADYGSGDTGTVCEWGVYTLNESIVPVAPTTWGALKSGIRFEN"
     gene            complement(3403..4365)
                     /locus_tag="VF720_14145"
     CDS             complement(3403..4365)
                     /locus_tag="VF720_14145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7880551.1"
                     /translation="MSSLTLAAAPAPTLRLTVLVPAYNEAESLPELHRQIRVELDKLT
                     GGTEILVIDDGSTDDTAGVVRALAGADSSVRLVSFRRNYGKSAALAVGFAEARGEIII
                     TMDADLQDDPAEIPRLLARLDEGFDVVSGWKQNRQDPIMKTVPSKLFNAVTSAACGLR
                     LHDFNCGLKAWRREAARSLEVYGELHRYLPALSHLQGFRVTEIPVKHHARRFGTTKFG
                     SNRFINGFLDLLAVMFLHSGSRSPLHVFGRVGLLFGFVGGGILAYFAVIWISGQWLKV
                     RPLLLFGVGLVILATQFISLGLLGEMIARGQQQDRTYAIRDRIG"
     gene            complement(4362..5342)
                     /locus_tag="VF720_14150"
     CDS             complement(4362..5342)
                     /locus_tag="VF720_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017290607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7880552.1"
                     /translation="MTTDTRPLALVTGAAGFIGSHLTQALLAKGWRVRGADCFTNYYP
                     AAFKKANLEAIGDSDDFEFNEIDLETADMDFLCDGVTVVFHQAAQPGVRASWGKEFEV
                     YLRQNIGVTQRLLEHFKEKPITKFVYASSSSVYGEAVSLPMKETDRPQPYSPYGVSKL
                     AAEHLCRLYTRNYALPTVSLRYFTVCGPRQRPDMAFHKLVRAMLRDEEFAIYGEGDQT
                     RDFTYVGDAVAANIAAAEVGKPGEVYNIGGGARISLKDAIALLEQAAGKKARVRFEEK
                     QKGDPSHTVADTTRAREDLGYDPKTPLAEALRLEYDWMADLLERRGSLPA"
     gene            complement(5353..7845)
                     /locus_tag="VF720_14155"
     CDS             complement(5353..7845)
                     /locus_tag="VF720_14155"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX7880553.1"
                     /translation="MPTSAEGRVNALIGALVLFFSLAVYWYCITPTVPYWDSGEYIAT
                     SYILGIPHPPGTPLYVLIGRLFTFLPLGSIAVRVNLLSAFSSAAAVLFTYLIIVRVSR
                     KNWPDAGWLAYAGGVVGALLMAWGTTFWDNAIEAEVYASASAIMCFCVWLGLRWWDGQ
                     QEAANDRIIWLILYVLFLAIGIHLGTFLVFPCIYLLVTMVHWDRVQKGAFWSSIGIFL
                     LAAFIRMGIIMNAHRQFDVTELSIAPEGFRTGASVMSLIMASAVAWNVVSVLGMRFAV
                     GIGALAVLGVSVHFYLLLRAQLNPAINEADPATWDALKLVLTRDQYKPPEPIFRKAEW
                     GYQFSQMYWRYFQWQFELFKAGNLPTYWVPILLGVFGAVQNWFREKKSFWLIATLFLI
                     TGPFLVYYLNFKEGEVRERDYFFVANFHFFAIWIGMGVVGLGRMLAGPEEAVERRDLF
                     KPAVVGVTLLAILMSVMPLAAGQDNQNLFRHNRRGNFVAHGYGHNMLAGLEPDAILFT
                     NGDNDTFPLWYMQEVEHFRKDVRVVNLSLLQTSWYIKQLRDYAPKVPMRLDDGQIDAL
                     TPYREKDGRIVYVNDIMVDHILEVNDYKRPVYYAVTVPDQRDLTPRLRMEGLVFRIYK
                     DRVADGIDVARLQDNLDHKYHYRGFLTPAGDYDTKVYKDEQATRLLQNYAAARVQLAV
                     GLNNEGKRDEARAQLDQVRKYARYFPGVDAALGATYAQIGLGDEAVTYYQDLLRTEPD
                     NASALAILGHLKVERGDTTSGVADLARAIELDPGGDFNPYADLANIYQGRGDAGQVVQ
                     LLNQWLQHHPDDTRVRNYVNAMTGRPAVTGGR"
     gene            complement(7937..>8697)
                     /locus_tag="VF720_14160"
     CDS             complement(7937..>8697)
                     /locus_tag="VF720_14160"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880554.1"
                     /translation="VVMVKDIMVQHILKANSWKRPIYLAVTVPDQMGLEKQLVFEGLV
                     FRVHPDPQARRMDTARTMDNLDNKFRYQGFLDANGDWDTTVYKDEQSTRLLQNYAAAR
                     VQLGLSTYEEGKLEESRIQLEKARKMSPHFPGVVAALGYVYERLSRWPDAAAHYEEQL
                     TAFPGDAVLMANLGRVKAHLGDTTAAVALLEKSIAAEPGTDFAPYTELVNILFARGDA
                     ARCVSLLEQWLRYHPDDQKAKSSLELVRSSMTHP"
BASE COUNT         1520 a         2860 c         2849 g         1468 t
ORIGIN      
        1 cccatcctct cggaacgaga acggcacgac ctgccccccg ccggcctcga gcttcccggc
       61 caccttgtgg cgggaatcga acggcacgta gatcaggaga tagccgccac cccccgcccc
      121 gaggatcttg ccgccgagcg cgccggcctt gcgggcctcg gcgtagagat cgtcgatccg
      181 ctcgttgctg atgcggtcgg agaggcgcgt cttttcctgc caggcggtgt gcagcaggct
      241 gccgaagtcg gccaggcggt cgcgcaggag gcacgacttc atctcgatcg tgagcgactt
      301 catccggtcg agcgcccgca cgaccggctt cttgcgatcg acataggcgc gggtctgctc
      361 ggccacgatg ccggccgatt cgcgcgtccg gccggtgaag cagagcagga ggttgtactc
      421 gagctcgcgc acggtggccg gcttgatgcg gagcgggttc accacggtga cgccgtcgtg
      481 gaactcgatg aagttgaaac cgccgaaggt ggccgcgtac tggtcctgcc gtcccccggc
      541 cagcttcagc tccacccgct cgatgagata agtgagctcg gcgatttcgt aatccgtgag
      601 gggctggcgc agccattccc gcatgagccc caccagcgcc accgtcatgg tggacgacga
      661 cccgagcccc gagccgggcg gagcatccga gtgcaggaag aggtcgaatc ccgacttcgg
      721 ctggaagtgg ttggccaccg ccttcacgat gtcgagctcg ccgtcgtagg caagcttctt
      781 ccctttcatg tggtaattgg ccacgaggtt gtaatcgagg gagtggatcg acatgcggac
      841 atcgcgccgc ggccggagcg tggcccacgc gaacttgtcg atcgtggtgg agaggacggc
      901 accacccttc tcggccggat aaggatcgac gtcggtgccg ccgccgcaga acgaaatgcg
      961 gagtggcgcg cgggcccgga acaggacacg ggacggcgcg tcgctggcgc ggcgggatgg
     1021 aacgacgggc ttacgactct ttcgggaagc catcaatcac tccgagataa tcctccggca
     1081 ggccgatgtc gacgaaatac gcatcggtca ccacgccacg cagggaggca tgtccccccc
     1141 ggttgatcca ggccggcagg atgtcacgct ccagggaaac cggtcggccg gcgggaatgt
     1201 cccgcacgcc acccttttcg atcaagtaga cgccggcatt gatcagcccc ggcccgacct
     1261 tccccttctc gaggaagcgc gtgatgaagc cctcctcgtt caactccacg ctccccttct
     1321 ggccggcgtc ggcaaatcgt gtcagggcca gcgtggccac cgggaccggc gcgctcagat
     1381 gccggacatc gatctcggaa agtcgcgttc gcagaaaggt gtctccgttg acgaggaaga
     1441 accgatcgtc gagcagtgcc agagcgtggc gcaatgcccc accggttccc agcggctccg
     1501 gctctacagc ggtagtgatg gcaaggtttc gtgccgacac atcattggcc gcggcaatca
     1561 ccttgtcggc gccgacgccg agaaggagca acgccctgct aattccttgc ccggcaagat
     1621 attcgagaag gtgatgaagg aagggacgcc cggctatcgg cagcagcgcc ttcggaagtc
     1681 cgcccgagag cgaggcgatc cgggtgccga ggccgccggc caggatgact gcctgtcgtg
     1741 tcgtcagatt ttgcacctgt cgttgaaagc ccacatcgcg gcgatcctca ccgcttcgac
     1801 ggatcggatc ctactggaat ttccataaag ttggttgcat tccggaaagc ggcgacctaa
     1861 cctgccgact gtcgccaatt gatcggctcg cgattgccac actcttcccc tggggcacgc
     1921 gacgtgcgcg gcacacttga aaggaggtcc ccacagccgc cgacgacggc ggactcgcgg
     1981 tcggtcccac cccgccccga caggatcttc atcacgacat cacacctttg gcaagtaccg
     2041 atcggggggt tccctcacga gcgcggcggg ccactcagag gcctctcgtc ccgctcaaac
     2101 ccaactgtcc ccatcccaag ccaggaggtc gtcttgagcc atcgtgaccg agtccttccc
     2161 acggtgaccg ccgccgcggt tgccctgttc ctcgcatccg ccgcccaggc cggacttccc
     2221 cgcatcgcct ccgaaaaggt tccgcagagc gacgaagtca agtgggacgt catggaaacc
     2281 ttcgacctga acgccaccct cgccgagatc gaaccgaacg acacccaggc ccaggcccag
     2341 ccgatgtcgt gcggcgacgt gatcaacccg gcctatctcg aggcgggggg attcgacgtc
     2401 tatcgcttca cggccacggc cggacagctc ctcacgctgg gcaccgactc gaacgggggg
     2461 ctcgataccg atacctggat ctacctcatc gacgccgcct tcacgacggc cctggcccag
     2521 gacgacgaca gcggacccgg cgcctactcg ctgatcagcg gctttgcggc cccctacacg
     2581 ggcacctatt actttgcggt ggaaggctat gacgtggaag tcgacgaggg agattaccgg
     2641 ggcttcgtca actgttcgag cacgccgcct cccaccaact gcagcatcac cgactacaag
     2701 gggcaggtgg tcacgctccc cacgccggtg gccattccgg acaacaacct ggccggcgtc
     2761 agcgtgggca ccatcaacac gcccaacgac ctgaccaagt tcctcgacgt gatcgtgagc
     2821 ctcaaggtca cgcacacctg ggtcggcgac ctggtagcca cgctgaccta cgacgagacc
     2881 tgtgacggcc agcccgaagc ctcctcgcgc ttcatctgtc gtcccggccg cgccacctgc
     2941 gacaacgtgc tcggcacgcc gttcggctgt tcgtcgaacc tcgcgtgcgc caacaacatg
     3001 ttcttcagcg acggcgccac caatgtcctg gggacgagcc cgaccggctg cggcacctcc
     3061 tccaccgtcc tggccgccgg ctgctggaag gggggcagca acgcccagcc cctctcggtg
     3121 ttcgacaact tccgcaaggg tggctgcttc aagctgaacg tggccgacta cggctcgggc
     3181 gataccggta ccgtctgcga gtggggcgtg tacacgctca acgagtcgat cgtgccggtg
     3241 gctcccacga cctggggcgc cctgaagtcg gggattcgct tcgaaaactg atcgattaca
     3301 ggtacaaaca gaatcggggg cggatcctcg ccgggtccgc ccctgattgt tgctgaaaaa
     3361 catgttcgat tgattgcggg cccgcacgcc ggggcaatga tttcagccga tccggtcgcg
     3421 gatggcgtag gttcgatcct gctgctggcc ccgggcgatc atttctccca gcaatccgag
     3481 ggagatgaat tgggtggcca ggatgacgag gccgacgccg aagagcagca acggtcttac
     3541 cttcagccac tggccgctga tccagatgac ggcaaaatag gccaggatgc cgccgccgac
     3601 gaagccgaac aggaggccga cgcggccgaa gacgtggagg gggcttcggg agccgctgtg
     3661 gaggaacatc acggccagga ggtcgaggaa gccgttgatg aatcggttgc tgccgaactt
     3721 ggtggttccg aagcgccggg cgtggtgctt gaccgggatt tcggtgacgc ggaacccctg
     3781 caggtgcgag agggcgggga ggtagcggtg gagctcgccg tacacctcga gggatcgcgc
     3841 cgcctcgcgg cgccaggcct tgagaccgca gttgaagtcg tgaaggcgga ggccgcacgc
     3901 ggcgctcgtg acggcgttga agagcttcga cggcaccgtc ttcatgatcg ggtcctggcg
     3961 gttctgcttc cagccgctca ccacgtcaaa cccttcgtcg aggcgcgcga ggaggcgcgg
     4021 aatttcggcc ggatcgtcct gcagatcggc atccatcgtg atgatgatct ccccgcgcgc
     4081 ctcggcgaag ccgacagcga gtgccgcgga tttgccgtaa ttccgccgga agctcaccag
     4141 cctcaccgag gagtccgcgc ccgccaacgc ccgcaccacc cccgccgtgt cgtccgtcga
     4201 cccgtcatcg atgacgagga tctcggtgcc gccggtcaac ttgtccagct ccacgcggat
     4261 ctgccggtgc agctccggca ggctctcggc ttcgttgtaa gccggaacga gcacggtgag
     4321 ccgcagtgtc ggcgcggggg cggcggccag cgtcagcgaa ctcaagccgg caggctcccg
     4381 cggcgttcca ggaggtcggc catccagtcg tactcgaggc ggagcgcctc ggccagcggc
     4441 gtcttcgggt cgtagcccag gtcttcgcgg gcgcgcgtgg tatccgccac cgtgtgggaa
     4501 ggatcccctt tctgcttttc ctcgaaacgg acgcgggcct tcttccccgc ggcctgctcc
     4561 agcagcgcga tggcgtcctt caacgagatg cgcgcgcccc cgccgatgtt gtacacctcg
     4621 cccggcttcc cgacttcggc cgcggcgatg ttggccgcca cggcatctcc cacgtaggtg
     4681 aagtcgcgcg tctggtcccc ttcgccgtag atggcgaatt cctcgtcgcg cagcatggcc
     4741 cggacgagct tgtggaacgc catgtcgggg cgctggcgcg ggccgcacac ggtgaagtag
     4801 cggagcgaca cggtggggag cgcatagttg cgcgtgtaga gccggcagag gtgctcggcg
     4861 gccagcttgg acacgccgta cgggctgtac ggctgcggcc gatcggtctc cttcatggga
     4921 aggctcaccg cctcgccgta caccgacgac gacgacgcgt acacgaactt cgtgatcggc
     4981 ttctccttga agtgctcgag gaggcgctgc gtgacgccga tgttctggcg caggtacacc
     5041 tcgaactcct tcccccagct ggcccgcacg cccggctggg ccgcctggtg gaacaccacc
     5101 gtcacgccgt cgcacaggaa gtccatgtcg gccgtttcca ggtcgatctc gttgaactcg
     5161 aagtcgtcgc tgtcgccgat ggcttcgagg ttggccttct tgaacgccgc cggatagtag
     5221 ttggtgaagc agtcggcgcc cctcacgcgc caccccttcg cgaggagagc ctgcgtcagg
     5281 tggctcccga tgaagccggc ggcgccggtc acgagggcca ggggtcgggt atcggtcgtc
     5341 atggcaatcc ttctagcgcc cgccggtcac ggccggacgc cccgtcatgg cgttcacgta
     5401 gttgcgcaca cgcgtgtcgt ccgggtgatg ctggagccac tggttcaaca gctgaaccac
     5461 ctgcccggca tcaccgcgcc cctggtagat gttggccagg tcggcgtacg gattgaagtc
     5521 cccgccggga tcgagctcga tggcccgggc aaggtcggcc acgccggagg tggtatctcc
     5581 ccgctctacc ttgaggtgcc ccaggatggc cagggcgctg gcattgtccg gctccgtgcg
     5641 gagcaggtcc tggtagtagg tgacggcctc gtccccgagg ccgatctggg cataggtggc
     5701 gccgagcgcg gcatccacgc ccgggaagta acgggcgtac ttgcgcacct ggtcgagctg
     5761 ggcccgggcc tcgtccctct tcccttcgtt gttcaggccc accgccagct gcacacgcgc
     5821 ggcggcgtag ttctgcagga gacgggtggc ctgctcgtcc ttgtacacct tcgtgtcgta
     5881 gtcgccggcc ggcgtgagga agccgcggta gtgatacttg tgatccaggt tgtcctgcaa
     5941 cctggccacg tcgatcccgt cggccacgcg atccttgtag atgcggaaga ccagcccctc
     6001 catccggagc ctcggcgtca ggtcgcgctg gtcgggcacg gtcacggcgt agtagacggg
     6061 gcgcttgtag tcgttcacct cgaggatgtg atcgaccatg atgtcgttca cgtagacgat
     6121 cctgccgtcc ttctcgcggt agggtgtgag ggcgtcgatc tggccgtcgt cgagccgcat
     6181 cggcaccttc ggcgcatagt cgcggagctg cttgatgtac cagctcgtct ggagcaggga
     6241 gaggttgacc acccgcacgt ccttccggaa gtgctccacc tcctgcatgt accagagcgg
     6301 aaaggtgtcg ttgtcgccgt tggtgaagag gatggcatcg ggctcgaggc cggccagcat
     6361 gttgtggccg tagccgtgcg ccacgaagtt gccgcggcga ttgtgacgga agaggttctg
     6421 gttgtcctgg ccggccgcca gcggcatcac cgacatcagg atggccagga gagtcacgcc
     6481 caccaccgcg ggcttgaaca ggtcacgacg ctccacggcc tcctccgggc cggccagcat
     6541 ccggccgagc cccaccaccc ccatgccgat ccagatggcg aagaagtgga agttggccac
     6601 gaagaaatag tcgcgctcgc ggacctcccc ctccttgaaa ttgaggtagt agacgaggaa
     6661 ggggccggtg atcaggaaga gcgtggcgat cagccagaac gacttcttct cgcggaacca
     6721 gttctgcacg gcgccgaaaa cgccgaggag gatcggaacc cagtacgtgg gaaggttccc
     6781 cgccttgaag agctcgaatt gccactggaa gtagcgccag tacatctggc tgaactggta
     6841 gccccattcg gccttccgga agatcggctc gggcggcttg tactggtcgc gcgtgagcac
     6901 cagcttcagg gcgtcccagg tggccgggtc ggcctcgttg atggcggggt tcagctgggc
     6961 ccgaagcagc aggtagaagt gcacgctcac cccgagcacc gccagcgccc cgattcccac
     7021 ggcaaagcgc atgccgagga ccgacaccac gttccaggcc acggcggacg ccatgatgag
     7081 cgacatgacc gaggcaccgg tcctgaaccc ctcgggggcg atggagagct cggtcacgtc
     7141 gaactgccgg tgggcgttca tgatgattcc catccggatg aacgccgcca ggaggaagat
     7201 gccgatcgac gaccagaagg cacccttctg gacgcggtcc cagtggacca tcgtcaccag
     7261 caggtagata cagggaaaga ccaggaacgt gccgagatgg atgccgatgg ccaggaagag
     7321 gacgtagagg atcagccaga tgatccggtc attggccgcc tcctgctgcc cgtcccacca
     7381 gcgcagtccc agccagacgc agaagcacat gatggcgctg gccgaggcgt acacctcggc
     7441 ctcgatggcg ttgtcccaga acgtggtgcc ccaggccatg aggagcgccc ccaccacgcc
     7501 gcccgcgtac gccagccagc cggcatcggg ccagttcttg cgggaaacgc gcacgatgat
     7561 gaggtaggtg aagaggacgg ccgccgccga ggagaacgcc gagaggaggt tcaccctgac
     7621 cgcgatcgag ccgagcggca ggaaggtgaa gaggcgcccg atcagcacgt agaggggcgt
     7681 gccgggcggg tgcggaatcc ccaggatgta cgaggtggcg atgtactcgc cggagtccca
     7741 gtagggcacc gtcggcgtga tgcaatacca gtagaccgcc aggctgaaga aaaggacgag
     7801 ggcgccgatc agggcattga cccgaccttc ggccgacgtc ggcatcacgg accacgccac
     7861 cccgccttgt ttcggcggcg cggcgactga cgaggcagcg ttcacgaaca tctcccggcg
     7921 ggcagcgcgc cccgcttcag gggtgggtca tcgaactccg gaccagttcc agcgaactct
     7981 tcgccttctg gtcgtcggga tgatagcgga gccattgctc gaggagggag acgcagcggg
     8041 cggcatcccc ccgggcgaac aggatattga ccagctcggt atacggggcg aaatcggtgc
     8101 cgggctcggc cgcgatcgac ttttcgagga gggcgaccgc cgcggtggtg tcccccaggt
     8161 gggccttgac ccgtcccagg ttggccatga gcaccgcgtc gcccggaaag gccgtgagct
     8221 gctcctcgta gtgcgccgcg gcatcgggcc agcggctcag tcgctcgtac acgtatccga
     8281 gcgccgccac cacccccggg aagtggggcg acatcttccg ggccttctcg agctggatgc
     8341 gcgattcctc cagcttcccc tcctcgtagg tggagagccc gagctgcacg cgcgcggccg
     8401 cgtagttctg cagcagccgg gtcgactgtt catccttgta caccgtggtg tcccagtctc
     8461 cgttggcatc gaggaagccc tggtagcgga acttgttgtc gaggttgtcc atcgtccgcg
     8521 ccgtgtccat ccgtcgggcc tgcgggtcgg ggtgcacgcg gaacaccagc ccttcgaaca
     8581 ccagctgttt ttcgaggccc atctggtccg gcaccgtcac cgccaggtag atcggccgct
     8641 tccaggaatt ggctttcagg atatgctgca ccatgatgtc tttgaccatc acgacct
//
LOCUS       DASVOD010000658         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_513001, whole genome shotgun sequence.
ACCESSION   DASVOD010000658 DASVOD010000000
VERSION     DASVOD010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513001"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF720_14165"
     CDS             complement(<1..629)
                     /locus_tag="VF720_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880555.1"
                     /translation="MTFRRTVLACLVVGAAIGAGMAWQQNGGSAQHPDDDASWHHRRD
                     PAVGDSIVAEYERSLGTWTSGPVTLRAPRNLLSVEEGAELARRCDVRLTAMTRRLVGG
                     TGPTAIPPTVVWCYPDFQTKGLRTGNSLVEHADPDSNVVHRVLERGRAEGDGLAEADL
                     VLRRANVRLTHSWLEAGALVWLADSWLGVPPAEWGATLEGMGVWPGTESL"
     gene            complement(646..1218)
                     /locus_tag="VF720_14170"
     CDS             complement(646..1218)
                     /locus_tag="VF720_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880556.1"
                     /translation="MVAGDESAVARLVGLLEHVDPRVRGGAADGLAALAEQDAALLVS
                     HVTALSMALSREETQTRVGAQRALAAVAGVAPASLEDEFDQIRLGAFDPANTDLRRYA
                     ALAIGHYGAGDDTRGRRAFPHLAEALRRFHDRARPADLLDGMLLLARSPASDWLKNEL
                     WKSARKHEAHTDTAVRDRVAEIGRLVRPTP"
     gene            1165..>2603
                     /locus_tag="VF720_14175"
     CDS             1165..>2603
                     /locus_tag="VF720_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880557.1"
                     /translation="MLEQADEARDGGLISRHHARHFGGGGTGALHATLQQNDQGIFHG
                     RIVCHNTRAAYNAAIMRMRNALAGVVWLVVSAGAVGPISPALADPPSGDRARPAGPPS
                     ASHLVNLAGATLPRLSPDGSRVAFVLADSLDPTLRQIALAVTEEGIRPRPLTAGGRSA
                     DNPRWTPDGKHLLFLTSASDTSLPQIRIIDPTGGESRTLTGEPRGVADMHVSPDGRLL
                     AWLVPGPLNPGGDPVDPMMRPPGTKLRWRELDGTKTTTLSPDSANVWRFAWAPDNRRV
                     AALYTRPGKFGEWRRGMLAIADIESGEWRRVPGLWDPNNDVAWSAGGDSLAAYRLPAL
                     DRAYPVLHVMSVDDLDASPANRITPPDARYTEHGVIWTSGGGLVVASWEGVRTFLTRI
                     DPASGRRTRLAECWNELSTDFSMNDAGVLAWISGGLDGPADIYARLPGDAASRRLSIL
                     NPQLAEREWPAPEVVTWRSRDDLTIEGIL"
BASE COUNT          376 a          970 c          876 g          381 t
ORIGIN      
        1 agagattcgg tccccggcca gactcccatc ccctccagtg ttgcacccca ttccgcgggc
       61 ggcaccccga gccaggagtc ggccagccag acgagcgcac cggcctcgag ccaggagtgc
      121 gtcaatcgaa cgtttgcgcg gcggagcacg agatcggcct cggccagccc gtccccttcg
      181 gcgcgaccgc gctccaggac tcgatggacg acgttcgagt cggggtcggc atgttccacg
      241 agactattgc cggtccgaag ccccttcgtc tggaaatcgg gatagcacca caccacggtt
      301 ggcgggatcg ccgtcggtcc ggtcccgccg acgaggcggc gggtcatggc cgtcagtcgt
      361 acgtcgcatc gccgggccaa ctcggcgccc tcctcgactg acaggaggtt gcgcggcgcc
      421 cgcagcgtga ccggaccgct ggtccacgtg ccgaggctcc gctcgtattc ggccacgatg
      481 gaatccccca ccgccgggtc gcgtcgatgg tgccatgacg cgtcgtcgtc ggggtgttga
      541 gccgaaccgc cgttctgctg ccacgccatc cccgccccga tcgccgcgcc caccacgagg
      601 caggcgagca cggtacggcg gaaggtcatg acggggagcg gcgggtcatg gcgtcgggcg
      661 gaccagtcgc ccgatttccg ccacgcggtc gcggacggcg gtgtcggtgt gggcttcgtg
      721 cttgcgggcc gacttccaga gttcgttctt gagccagtcg ctggcgggag agcgtgccag
      781 gaggagcatc ccgtcgagga gatcggccgg gcgggcgcga tcgtggaaac gacggagtgc
      841 ctcggccagg tgcgggaagg cacgtcggcc acgcgtgtca tcaccggcgc cgtagtgacc
      901 gatggccagg gccgcgtaac ggcggaggtc ggtgttggcc ggatcgaagg cgccgaggcg
      961 gatctggtcg aactcgtctt ccagtgaggc cggcgcgacg ccggccaccg ccgccagcgc
     1021 gcgttgcgcg ccgacacgcg tctgtgtttc ctcgcgcgac agggccatcg aaagcgcggt
     1081 cacgtgcgag acgaggagcg cggcatcctg ctcggccagc gccgccaggc cgtcggccgc
     1141 accgccccgc acgcggggat cgacgtgctc gagcaggccg acgaggcgcg cgacggcgga
     1201 ctcatctccc gccaccatgc ccgccacttc ggcggcggtg gcaccggggc gctgcacgcc
     1261 accctgcaac agaacgacca ggggatcttt catggccgga tagtgtgcca caacacccgc
     1321 gccgcctata acgccgcgat catgcggatg cggaacgccc tggccggtgt cgtgtggctc
     1381 gtcgtctcgg ccggcgccgt cgggccgata tcgccggccc tggccgatcc tccctccggc
     1441 gacagggccc gccccgccgg tcccccttcc gcctcccatc tcgtcaacct cgccggcgcc
     1501 accctgccgc gcctctcccc cgacggcagc cgcgtggcct tcgtcctggc cgacagcctc
     1561 gatcccacgc ttcgccagat cgccctcgcc gtcaccgagg agggaatccg gccccggccc
     1621 ctcaccgccg gtggccgaag cgccgacaat ccccgctgga caccggacgg aaagcatctc
     1681 ctcttcctca cttccgcgag cgacacctcc ctgccgcaga tccgcatcat cgacccgacg
     1741 ggcggcgaga gccgcacact caccggcgag ccccgcggcg tggccgacat gcacgtctcc
     1801 ccggacggcc gcctgctggc ctggctggtg cccggtccgc tcaaccccgg cggcgatccg
     1861 gtcgacccga tgatgcgacc gcccggcacg aagctccgct ggcgcgagct cgatggaacg
     1921 aagacgacaa ccctctcgcc cgactccgcc aacgtgtggc gtttcgcgtg ggcgcccgac
     1981 aaccggcgcg ttgccgcgct ctacacgcgc cccggcaagt tcggcgagtg gcgacgcggc
     2041 atgctggcca tcgccgacat cgaatccggc gagtggcgac gtgtgccggg gttgtgggac
     2101 ccgaataatg atgtggcctg gagcgccggc ggcgattccc tggcggccta ccggctcccc
     2161 gcgttggacc gcgcctaccc cgtgctccat gtcatgagtg tggatgacct cgatgcaagt
     2221 ccagcgaacc ggatcacccc tcccgacgcc cgctacaccg agcacggcgt catttggact
     2281 tccggcggcg gcctcgtggt ggcgtcctgg gaaggggtgc ggacgtttct gacgcggatc
     2341 gatccggcca gcggccgccg gacccgcctc gccgaatgct ggaacgaact cagcaccgac
     2401 ttctcgatga acgatgccgg cgtgctcgcc tggatctcgg gtgggctcga cggtccggcc
     2461 gacatctacg cgcgccttcc cggcgacgcg gcctcccgcc gcctgagcat cctcaatccc
     2521 cagctggccg agcgggagtg gcccgccccg gaggtcgtca cctggcgttc cagggacgac
     2581 ctcacgatcg agggaatcct ttt
//
LOCUS       DASVOD010000659         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1039185, whole genome shotgun sequence.
ACCESSION   DASVOD010000659 DASVOD010000000
VERSION     DASVOD010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039185"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VF720_14180"
     CDS             complement(<1..568)
                     /locus_tag="VF720_14180"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX7880558.1"
                     /translation="MSRFTATGPSRGPSRPRHAAAVILLRNPLDPEIFWVRRQPHLAF
                     LGGFHSFPGGRMGKEDDPALLGMTGEATEAEQLDASLRLCALRELHEETGVSLRDPIA
                     SGVAFLDAVRNGAHRVPVEALVPAGRWVTPPFSPARFDTTFYLAWIPERVEPVVHDSE
                     LTDGEWIRPDAALDRWSAGKALLAPPALY"
     gene            complement(646..>2916)
                     /locus_tag="VF720_14185"
     CDS             complement(646..>2916)
                     /locus_tag="VF720_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7880559.1"
                     /translation="NTRLLNIWDQTVATGPNPAGFAYGTEWTPAQINANTCTQTDTQQ
                     HGTHVMGSMGGDGSAVGTGSAPAFTYAGMAPKADLIMVKTNFQTTGVTDGVTYIMNKG
                     TALGKNTVVNLSLGSHFGPHDGTSPFETALSAVSGPGKIVVKSAGNERGVAQHAEVFA
                     AGAGTSATMTVTGSAAGRTIAIDGYYEASENISVRVTTPSGQVLGPYALGTINAAYPG
                     VNTTSNGVLYVENGAFLTATGDRQVYIELNRNSLAPGNGTTNGTWTFTFIPVALGAAN
                     GEVDLWRFFQSTSHTGNFVTGNQATEELVSEPGNSVEVITVAAWTSKTNWIACTGTNT
                     TFTGTVINTLASFSSPGPTRDGRQKPDIAAPGIAIISSRSDDLVQSCPASGATTLLPG
                     LKHIANAGTSMAAPHVSGAVALLMQKFGAITPAFAKMHLTNDALVDGNTGAVWNKDWG
                     MGKLRVDVTNPTASVTVPNGAEIWTVGSPVNITWSAADPLISGNPVTINIALSRTGLG
                     GPFSEALATGIVNSGSFGWNVTGPITTNAAIRITAIDPCTNQGIDISDAVFTIANAPV
                     PVKLAVFTAETVEGGVKLRWQYSDDSDFATTLVERSTLVDGTYELLAAPIASEAGASV
                     VVDETAEPGITYFYRLVATERTGTVTRFGPMSAVSRGFTKFALAPTWPNPTAGKSRVE
                     FTVPVAGRASLVVYDVTGRVISTVASGDFAAGKHQAEWSGATDSGSPAANGIYYLALE
                     SPNLDSRVVQRITLSR"
BASE COUNT          440 a          914 c         1088 g          474 t
ORIGIN      
        1 catacagggc aggcggcgca agcagcgcct tccccgcgct ccagcgatcg agggccgcgt
       61 cgggacggat ccactcgccg tcggtcaact cgctgtcgtg gacgaccggc tccacccgct
      121 ccgggatcca tgccaggtag aaggtcgtgt cgaagcgggc cgggctgaag ggaggcgtca
      181 cccagcggcc ggccgggacg agcgcctcga ccggcacccg gtgagcaccg ttccgcaccg
      241 cgtcaaggaa cgcaacgccg gaagcgatgg gatccctcag gctcacgcct gtctcctcgt
      301 ggagttcgcg cagcgcgcag aggcgcagcg aggcatcgag ctgttcggcc tcggttgcct
      361 cgcccgtcat gccgaggagc gccggatcgt cctccttccc catccgcccc cctggaaacg
      421 aatggaagcc gccgaggaac gcgaggtgcg gttgacgtcg aacccagaag atctccggat
      481 cgagcggatt ccgcaggagg atgaccgcgg cggcgtgccg ggggcgggag ggcccgcgag
      541 agggccccgt ggcggtgaac cggctcatga ggcgggagaa tacacgaagg gccgggctgc
      601 caaagcagcc cggccccgcg tgatcaactg gaaagactgg tgtacttacc ggctcagcgt
      661 gatccgctgc acgacgcgcg agtcgaggtt cggcgactcg agtgccaggt agtagatccc
      721 gttggcggcc ggcgaaccgc tgtcggtggc accgctccac tcggcctggt gcttgccggc
      781 ggcgaagtcg ccactggcca ccgtcgagat gacgcggccc gtgacgtcgt acaccaccag
      841 gctggcacga ccggccaccg gaacggtgaa ctcgacccgg ctcttgcccg cggtcggatt
      901 cggccacgtc ggcgccagcg cgaacttggt gaagccgcgc gagacggccg acatcggacc
      961 gaagcgggtc acggtgccgg tgcgctcggt ggccaccagg cggtagaagt aggtgatgcc
     1021 cggctcggcc gtctcgtcca cgaccaccga cgcacccgct tcggacgcga tcggggcggc
     1081 gaggagctcg tacgtgccgt cgaccagcgt ggagcgctcc acgagggtcg tggcgaagtc
     1141 cgagtcgtcc gagtactgcc agcgaagctt cacgccacct tcgaccgtct cggccgtgaa
     1201 cacggccagc ttcaccggga ccggcgcatt cgcgatcgtg aagacggcgt cggagatgtc
     1261 gatcccctgg ttggtgcagg gatcgatggc cgtgatccgg attgccgcgt tggtggtgat
     1321 cggaccggtg acgttccagc cgaaggagcc ggagttcacg atccccgtgg ccagggcctc
     1381 gctgaacgga ccgccgaggc cggtgcggga gagggcgatg ttgatcgtga ccgggttgcc
     1441 ggagatcaac ggatccgcgg ccgaccaggt gatgttcacc ggcgaaccga cggtccagat
     1501 ctcggcgccg ttgggcaccg tgacggaagc cgtcggattg gtgacgtcga cccgcagctt
     1561 gcccatgccc cagtccttgt tccagaccgc accggtgttg ccgtcgacga gcgcgtcatt
     1621 ggtgaggtgc atcttggcga aggccggcgt gatcgctccg aacttctgca tcaggagcgc
     1681 cacggcgccc gagacatgcg gggcggccat ggaggtgccg gcgttggcga tgtgcttgag
     1741 gccagggagc agcgtggtgg cacccgacgc cgggcagctc tgcaccaggt cgtccgagcg
     1801 cgacgagatg atggcgatgc cgggagccgc gatgtcgggc ttctggcggc cgtcgcgggt
     1861 cgggccgggg ctggagaagc tggccagcgt gttgatgacg gtgccggtga aggtggtgtt
     1921 ggtgcccgta caggcgatcc agttggtctt gctggtccac gcggcgacgg tgatgacctc
     1981 gaccgagtta cccggctcgg agacgagctc ctcggtggcc tggttgccgg tcacgaagtt
     2041 gccggtgtgg ctggtcgact ggaagaagcg ccagaggtcc acttcgccgt tggcggcgcc
     2101 gagcgccacc gggatgaagg tgaatgtcca ggtgccgttg gtggtgccgt tgccgggcgc
     2161 cagcgaattg cggttgagct cgatgtacac ctggcggtcg ccggtggcgg taaggaacgc
     2221 cccgttctcg acgtagagca cgccgttcga ggtggtgttc acaccgggat aggcggcgtt
     2281 gatcgtgccg agggcgtacg gcccgaggac ctggcccgac ggcgtggtga cgcggacgct
     2341 gatgttttcc gacgcctcgt aatagccgtc gatggcgatg gtgcggccgg cggccgaacc
     2401 ggtcaccgtc atcgtggcgc tggtgccggc gcccgcggcg aagacctcgg cgtgctgcgc
     2461 gacgccgcgc tcgttgccgg ccgacttcac gacgatcttg ccggggccgg agacggcaga
     2521 cagggcggtc tcgaaggggc tcgtgccgtc gtgcggtccg aagtgcgagc cgagcgacag
     2581 gttgaccacg gtgttcttac cgagcgccgt ccccttgttc atgatgtagg tgacgccgtc
     2641 ggtgacgccg gtcgtctgga agttcgtctt caccatgatg aggtcggcct tgggcgccat
     2701 gcccgcgtag gtgaaggccg gagcgctgcc ggtgccgacc gcgctgccgt cgccgcccat
     2761 cgagcccatc acgtgcgtgc cgtgctgctg ggtgtcggtc tgggtgcagg tgttggcgtt
     2821 gatctgggcc ggtgtccact cggtgccgta ggcaaagccg gccgggttcg ggccggtggc
     2881 cacggtctgg tcccagatgt tcaggaggcg cgtgtt
//
LOCUS       DASVOD010000660         5444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_513319, whole genome shotgun sequence.
ACCESSION   DASVOD010000660 DASVOD010000000
VERSION     DASVOD010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513319"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VF720_14190"
     CDS             <1..874
                     /locus_tag="VF720_14190"
                     /inference="COORDINATES: protein motif:HMM:NF012443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEX7880560.1"
                     /translation="GADTLTGAGFFGMNDLNELDRTFLVERHLISRDLSQGSRARGVL
                     VAPGESLSIMVNEEDHLRLQAVASGFDLAEVWGRVSDLDDRLDKELDYAYRTDYGYLT
                     ACPTNVGTGMRASVLIHLPALVLTKQLKKVLQGVAAVGLAVRGFYGEGTDVMGNFFQV
                     SNQQTLGRAELEILENLERITNQLIEFEGKAREAMLRTARLQVEDKVWRAYGTLRYSR
                     SIASQEVISLSSAVRFGLAMGIEGLTDLMTLNEIMVYSQPAHIQELAGRPLEPSERNI
                     FRADMIRRRLANGR"
     gene            999..3461
                     /locus_tag="VF720_14195"
     CDS             999..3461
                     /locus_tag="VF720_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX7880561.1"
                     /translation="MHDKFTERVRKVMYLAREEAARLQHDYIGTEHLLLGLIREGEGI
                     AANVLSNLGLDLDVIRQAVESMVASPGGTLTIGEIPFTPKAKRVLELSIDEARQLGHN
                     YVGTEHLLLGLIREGEGVAARVLLELGVDRKKVREETLRLLGGTPTSNRPADEEKADT
                     PALNQFGRDLTMLAREAKLDPIIGRENEIERVIQVLSRRKKNNPILIGEPGVGKTAIA
                     EGLAMKIVEGKVPETLRDKRIVTLDLAAVVAGTKYRGQFEERLKAVMNEIRESKDTIV
                     FIDELHTIVGAGGAEGAIDASNMLKPALARGELQCIGATTLDEFRKYIEKDGALERRF
                     QPIMVEPPTADEAILIIRGLRDKYEAHHGVKFTDEAIVASVKLSDRYMSARFLPDKAI
                     DVLDESGARSRLSMSVVPPDLRAMEKKIEELAKEKESAIQAQEFEKAASFRDKEKEMR
                     KQLAKLKREWHESKTMKTPTVDAEDIAVVISKMTGIPASRLEESESTKLLRMEEEMKK
                     KIVGQDEAVSAVAKAVRRNRAGLRDPRRPIGTFIFLGPTGVGKTELARVLAQHLFDDP
                     DALIRIDMSEYMEKFSVSRLVGAPPGYVGYEEGGQLTEKVRRKPYSVVLLDEVENAHP
                     DVFNMLLQVLEDGTLTDSMRRKVNFKNTVLIMTSNLGARQIGGRSQLGFGKSDEQSTY
                     DKMKGTVIEEVRRTFNPEFLNRIDDLIVFRALGRKEMEQIVHILFEEVTKRVAEQGLH
                     LAITPPAAALLVEKGFDPNLGARPLRRAIQRLVEDPLAMLVLEKKFKPGSQVRISRKG
                     DELVFEEKATGDLNAEKVGRGA"
     gene            3433..>5444
                     /gene="bamA"
                     /locus_tag="VF720_14200"
     CDS             3433..>5444
                     /gene="bamA"
                     /locus_tag="VF720_14200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX7880562.1"
                     /translation="MPRRSVAAPDAPERLTPLIICCLLALGLLGASSLLFTPGNSWAQ
                     GEPRVRKIAVRGVETVDTLRVITGLGVNRGDLYRPAAVRDGVKALYRLDLFEQVEVEA
                     EPTAGDSVDLVVTVVELPRVSAVEFVGNKGLDADKLKEKLTGFTSRTAGTRTQRDAQT
                     ALTELYREEGYPLAEVEASFAPGPKPSDRILSLNVKEGNRVQVATIDFDGNQRLPADK
                     LRDKLDTKKKSFWHKGHLKSATLEADQEKLRAYYQENGYRDARVTGYEVNYSEDKRLA
                     GVVFHIVEGPVYTMAPARIEGNTIIPTSALQTLVKFRTGQHYNRKKVDETAGEIGGAY
                     ADRGYLYSQVDPEETVDSTRVSVVYRIQEQEASKVREIRITGNTRTKEKVIRRQLYLY
                     PGSRFDRALLIRSQRELFQLGFFQDVQVDFRPIPNSFDVDLVLNVEEKSVGTASAGAG
                     FSSQGGLTGFLELGHPNLFGNGQAINIRLERGAKTSNYDISFTEPWFMGSPTTLGIDV
                     FKSNYIRDLYQVQRIGTSLRFGRPVPGMPYTRMFGTYSFERTSGLGRVTIDETLHTAY
                     SPDDSSSYVQTLTTSTPDPVGIDPSTRSASGITVGLSRNSTDHPIYPRAGSVSSLSLE
                     GNGGLFQGNVKFQKLLFDGRFFFKDQAFLPVSKPAMMFRAQFGATA"
BASE COUNT         1070 a         1819 c         1728 g          827 t
ORIGIN      
        1 cggggccgac acgctgacgg gggccggctt cttcggcatg aacgacctga acgagctcga
       61 ccgtaccttc ctcgtggagc ggcacctgat cagccgcgac ctctcgcagg ggagccgcgc
      121 ccgcggcgtg ctcgtggcgc cgggcgagtc gctctccatc atggtcaacg aggaagacca
      181 cctgcgcctc caggccgtgg cctcggggtt cgacctggcc gaggtatggg gacgggtgag
      241 cgacctcgat gaccggctcg acaaggagct cgactacgcg tatcgcaccg actacggcta
      301 tctcacggcc tgccccacca acgtgggcac cgggatgcgg gcttccgtcc tcatccacct
      361 gccggcactg gtgctgacaa agcaattgaa gaaggtcctg caaggtgttg ccgccgttgg
      421 tcttgccgtt cgcggcttct acggcgaggg gaccgacgtc atgggtaact tcttccaggt
      481 gtcgaaccag cagacgctgg gtcgggccga actcgaaatc ctcgagaatc ttgaacggat
      541 cacgaaccaa ttgatcgaat tcgagggaaa ggcccgcgag gccatgctca ggacggcccg
      601 cctgcaggtc gaagacaagg tgtggcgggc ctacggcacg ctgcgctaca gccgctcgat
      661 cgcctcgcag gaggtgatca gcctgtcgag cgcggtgcgc tttggactgg cgatggggat
      721 cgaggggctg accgacctca tgacactgaa cgaaatcatg gtttattctc agccggctca
      781 cattcaggag ctggcgggtc ggccactcga accgtcggag cggaacatct tccgtgccga
      841 catgattcgt cggcgactgg cgaacggacg gtaggaccgg gagcgatcgt cccggaaccg
      901 cccgaaacga caggggttta gcgcaccgcc gaaacggaac gcacgtgggc cggaccgaag
      961 cgcaggtcac gcgcaggggt ccccggaggt cgaacgagat gcacgacaag tttaccgaac
     1021 gggttcgcaa ggtcatgtac ctcgcgcgcg aggaagcggc ccggcttcag cacgactaca
     1081 tcggaacgga gcatctcctc ctcggcctca tccgcgaagg ggaagggatc gccgccaacg
     1141 ttctctccaa cctgggcctc gacctcgacg tcatccgtca ggccgtggaa agcatggtgg
     1201 ccagtccggg cggcacgctc accatcggcg agattccctt cacgcccaag gccaagcgcg
     1261 tcctcgaact ttccatcgac gaggcccggc agctcggcca caactacgtc ggcaccgagc
     1321 atctcctcct ggggctcatc cgcgaagggg aaggggtggc ggcccgcgtc ctgctcgagc
     1381 tcggcgtcga ccgcaagaag gtgcgcgagg aaacgctccg gctcctgggg ggcacgccca
     1441 ccagcaaccg ccccgccgac gaggagaagg ccgatacccc ggcgctcaac cagttcggcc
     1501 gcgacctcac catgctggcc cgcgaggcca agctcgaccc gatcatcggc cgtgaaaacg
     1561 agatcgagcg ggtcatccag gtgctgtcgc gccgcaagaa gaacaatccc atcctcatcg
     1621 gcgagccggg cgttggcaag acggccatcg ccgaggggct ggccatgaag atcgtggaag
     1681 ggaaggtccc cgagacgctg cgcgacaagc gcatcgtcac gctcgacctg gcggccgtgg
     1741 tggccggcac caagtaccgc ggccagttcg aggagcggct caaggccgtc atgaacgaga
     1801 tccgcgagtc gaaggacacc atcgtcttca tcgacgagct ccacaccatc gtgggggccg
     1861 gcggggccga gggggccatc gacgcctcca acatgctgaa gcccgcgctg gcccgcggcg
     1921 agctccagtg catcggcgcc accacgctcg acgagttccg caagtacatc gagaaggacg
     1981 gcgccctcga gcgccgcttc cagccgatca tggtcgagcc ccccacggcc gacgaagcca
     2041 tcctcatcat ccgtgggctg cgcgacaagt acgaggcgca ccacggcgtc aagttcaccg
     2101 acgaagccat cgtggcctcg gtcaagctct ccgaccgcta catgtcggcc cgcttcctgc
     2161 cggacaaggc catcgacgtc ctcgacgagt caggcgccag atcaaggctc tcgatgtcgg
     2221 tcgtgccgcc cgacctccgg gccatggaaa agaagatcga ggagctggcc aaggaaaagg
     2281 aatcggccat ccaggcccag gagttcgaga aggccgcctc gttccgcgac aaggaaaagg
     2341 aaatgcgcaa gcagctggcc aagctgaagc gcgagtggca cgaatccaag acgatgaaga
     2401 ccccgacggt cgatgcggaa gacatcgccg tcgtcatctc caagatgacc ggcatcccgg
     2461 ccagccggct cgaggaatcc gagtcgacca agctcctccg gatggaggag gagatgaaga
     2521 agaagatcgt cggccaggac gaggcggtca gtgcggtggc caaggcggtc cgtcgcaacc
     2581 gggccggcct gcgcgacccg cgccgtccga tcggcacctt catcttcctc ggccccaccg
     2641 gcgtgggcaa gaccgagctg gccagggtcc tcgcacagca cctgttcgac gatcccgatg
     2701 cgctgatccg catcgacatg tccgaataca tggagaagtt ctcggtcagc cgcctggtcg
     2761 gcgcccctcc ggggtacgtc ggctacgaag aggggggcca gcttaccgag aaggtgcggc
     2821 gcaagcccta cagcgtcgtg ctgctcgatg aagtggagaa tgcgcacccc gatgtcttca
     2881 acatgctgct ccaggtgctg gaagacggca cgctcaccga cagcatgcgc cggaaggtca
     2941 atttcaagaa caccgtcctc atcatgacca gcaacctggg ggcccggcag atcggtgggc
     3001 gcagccagct cgggttcggc aaatcggacg agcagtcgac gtacgacaag atgaagggta
     3061 cggtcatcga agaggtgcga cggaccttca atccggaatt cctcaaccgc atcgacgatc
     3121 tcatcgtctt ccgcgccctg ggccggaagg aaatggagca gatcgtccac atcctcttcg
     3181 aggaggtcac gaagagagtc gccgaacagg gactgcacct ggccatcacg ccgccggctg
     3241 ccgccctgct cgtcgagaaa ggcttcgatc cgaacctggg cgcccgcccg ctccgccgcg
     3301 ccatccagcg cctggtggag gatccgctgg cgatgctggt tctggagaag aagttcaagc
     3361 cgggaagcca ggtccggatc agccgaaaag gcgacgaact ggttttcgag gagaaggcta
     3421 cgggggacct gaatgccgag aaggtcggtc gcggcgcctg acgcgccgga gcgtctcaca
     3481 cccctcatca tctgctgcct gctcgccctg ggcctgctcg gggcgagcag tcttttgttc
     3541 actccgggaa actcgtgggc gcagggagaa ccccgcgtcc ggaagatcgc cgtccgcggc
     3601 gtggaaaccg tcgacacact gcgggtcatc accggcctgg gcgtcaaccg gggcgacctc
     3661 taccgcccgg ccgccgtccg cgacggggtg aaggcgctct accggctcga tctgttcgaa
     3721 caggtcgagg tggaggccga accgacggcg ggcgactcgg tcgacctcgt cgtcaccgtc
     3781 gtcgagctcc cgcgcgtgtc cgccgtggaa ttcgtcggca acaaggggct cgacgccgac
     3841 aagctgaagg aaaagctcac cggattcacc agccgcaccg ccggcacgcg cacccagcgt
     3901 gacgcgcaga cggccctcac cgagctctat cgggaggagg gttatcccct ggccgaagtc
     3961 gaagccagct tcgctcccgg gcccaagcct tccgaccgca tcctctccct gaacgtgaaa
     4021 gaggggaacc gcgtgcaggt ggccaccatc gacttcgacg gaaaccagcg gctccccgcc
     4081 gacaagctca gggacaagct cgacaccaag aagaaatcgt tctggcacaa ggggcacctg
     4141 aagtcggcca cgctcgaagc cgaccaggaa aagttgcggg cctactacca ggagaacggt
     4201 taccgcgatg cccgcgtgac cggctatgag gtcaactatt cggaagacaa gcgcctggcc
     4261 ggcgtggtct tccacatcgt ggaaggcccc gtgtacacga tggctccggc ccgcatcgag
     4321 gggaacacca tcatccccac cagtgccctg cagaccctgg tcaagtttcg caccggccag
     4381 cactacaacc ggaagaaagt ggacgagacg gcgggggaga tcggcggcgc ctacgcggac
     4441 cgcggttacc tttacagtca ggtcgacccg gaagaaacgg tcgactcgac gcgtgtgtcg
     4501 gtggtgtacc gcatccagga gcaggaggct tccaaggtcc gcgagatccg gatcaccggc
     4561 aacacgcgca ccaaggaaaa ggtgatccgc cgccagctct atctctaccc gggaagtcgg
     4621 ttcgaccggg cgctcctcat ccggagccag cgcgagcttt tccagctggg gttcttccag
     4681 gatgtgcagg tcgatttccg gccgatcccg aacagcttcg acgtggacct cgtgctcaac
     4741 gtcgaggaaa agtcggtcgg cacggcctcg gccggcgccg gcttttccag ccagggtggc
     4801 ctgaccggct tcctcgagct ggggcatccc aacctcttcg gcaacggcca ggccatcaac
     4861 atccgcctgg agcggggcgc caagacctcg aactacgaca tctcgttcac cgagccctgg
     4921 ttcatggggt cgcccaccac actcggcatc gacgtcttca agtccaacta catccgcgac
     4981 ctgtaccagg tgcagcgcat cggcacctcg ctccgctttg gccggccggt gccgggaatg
     5041 ccctataccc gcatgttcgg cacctactcg ttcgagcgca ccagcggcct cgggcgcgtc
     5101 acgatcgacg agacgctcca taccgcctac tcgcccgacg actcctcgtc gtacgtgcag
     5161 acgctcacga cctccacgcc ggacccggtc ggcatcgacc cttccacccg cagcgcatcg
     5221 gggatcacgg tgggcctttc gcgcaattcc accgatcatc cgatctaccc gcgggcgggc
     5281 tcggtctcga gcctgtcgct ggaagggaac ggcgggctct tccaggggaa cgtgaagttc
     5341 cagaagctgc tcttcgacgg ccgattcttc ttcaaggacc aggcgttcct gccggtcagc
     5401 aagccggcca tgatgttccg cgcccagttc ggcgccaccg cctt
//
LOCUS       DASVOD010000661         4026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_668668, whole genome shotgun sequence.
ACCESSION   DASVOD010000661 DASVOD010000000
VERSION     DASVOD010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668668"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VF720_14205"
     CDS             <1..764
                     /locus_tag="VF720_14205"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HEX7880563.1"
                     /translation="LQADDMRRRMSQELATATGIQRGMLPARPPEAMGWRFHARLESC
                     YEVGGDLYDFNVGPDGKLGLVVGDVSGKGMGAALLMSSFLSSSRTLFDMGLMPGDLVT
                     RLNLQMFRNSDPAHFVTAFIGRLDTATGHLDFVNAGHPPPFLIGPTGVRTLDATGVPV
                     GMLEDFPFTVGSVQMEPGDLLTVFTDGIPEAKRGDDFFEDERLEALLKELAPETDIDE
                     LGPELIRRVDEFLAGTPRSDDITLMLVRRDGTQTV"
     gene            1188..2267
                     /locus_tag="VF720_14210"
     CDS             1188..2267
                     /locus_tag="VF720_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7880564.1"
                     /translation="MHPRLVQFEEPVAGIDPAAPSEVVTPSDAIEVPIIDSNLSSETE
                     LLDAYSSAVIRVAEKVSPAVIRLDVLQRVESKRQGQAVERPGSGSGFFFTPDGFALTN
                     SHVVRGATKIEAILSDGRRVPARLVGEDPDTDLAVVRVTGNGFAALTLGDSESLKVGQ
                     IAIAIGNPFGFDCTVTAGVVSALGRSLRSQSGRLIDDVLQTDASLNPGNSGGPLVDSH
                     GRVIGVNTAIIRPAQGICFAVSVNTARFIASRLMRDGVVHRGKLGLAGQYVALPRYFV
                     RSLGLSRPGGVRVMSVEHGGPADLAGLKEGDVIVHLAGEAVAGIDDLHRLLTDGRVDQ
                     PLPITVVRAFEKMEFEVTPARRAAA"
     gene            2526..3044
                     /locus_tag="VF720_14215"
     CDS             2526..3044
                     /locus_tag="VF720_14215"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7880565.1"
                     /translation="MTRFVPALALAILIALAIAPPTRAATTEIPVVRIENTTTIAAAP
                     AAVWTWLTTGKNLITWCPVWKSESNKSVHLTKVGDALDFTDQWGNGGRSVVTFIAPNK
                     ELRVAHEPTDGSYLCQSRVILKPAGSGTEVTWIEQYTDASSATDKAATATKMETEMDA
                     TLGTLKASVETK"
     gene            3064..>4026
                     /locus_tag="VF720_14220"
     CDS             3064..>4026
                     /locus_tag="VF720_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX7880566.1"
                     /translation="MPVLPTACPLDCPDHCTLDVTVEEGRVVRVNDRAENPITAGFIC
                     GKVRRIDRHLYGKDRLRYPGVREGKKGEGRFRRVSWDEALGIIADKFGAIRASSGGEA
                     ILPYYYGGSNGALTQDATDATLFRRLGTTRILRTLCAAATGRATQGLYGKMPGISYLD
                     FEKAEVIILWGQNPSASSIHLVPILRRAMDNGTKLIVVDPRATPLARLAHRHLAVRPG
                     TDLPLALAIARWLFTNGRADEAFLAQHVSGVEAFRDRAMRWTLEETARVTGLAAADIE
                     ATAAEYAAANPALIRCGWGLERNRNGGSAVASVIALPAIAGKFGV"
BASE COUNT          650 a         1404 c         1303 g          669 t
ORIGIN      
        1 tcctccaggc cgacgacatg cgccgccgca tgtcgcagga actggccacg gccaccggca
       61 tccagcgcgg catgctcccg gcgcggcccc ccgaggccat ggggtggcga ttccacgcgc
      121 gcctcgagtc atgctacgag gtggggggag atctgtacga cttcaacgtc ggtcccgacg
      181 gcaagctcgg cctggtcgtc ggcgatgttt ccggcaaggg gatgggcgcc gcgctcctca
      241 tgtccagctt cctctcgtcc tcgcgcacgc tcttcgacat gggcctcatg cccggcgatc
      301 tcgtcacgcg cctcaatctc cagatgttcc gcaacagcga ccccgcccac ttcgtcacgg
      361 ccttcatcgg ccgcctcgat accgccaccg gccatctcga cttcgtcaac gccggccatc
      421 cgccgccttt cctgataggc cccaccggcg tccgcacgct cgatgccacc ggtgtccccg
      481 tcggcatgct cgaggatttc ccgttcaccg tcggctccgt gcagatggag cccggcgacc
      541 tgctcaccgt cttcaccgac ggcatcccgg aagccaagcg cggcgacgac ttcttcgagg
      601 acgaacgcct cgaggccctc ctcaaggagt tggccccgga gaccgacatc gacgaactcg
      661 gccccgagct gatccgccgg gtggacgaat tcctcgcggg tacgccgcgc tcggacgaca
      721 tcaccctgat gctcgtgcgg cgggacggca cgcagacggt gtgatgggtg tgacctggag
      781 gcgcggcatg ctggtcgcag gcctggcttc ggtcgccgtc ttatgcgcct gctcggagga
      841 tgagccggcc ggcggtttct gtgccgaaat accgggaaga gtcggcgtgg aattccacag
      901 cgaggttcgg gccgactcag tcgaggcaat tctgcaacgc ctcgagctga cctgggagcc
      961 agagacgatc cgggattgcg ccaccctgat cttcgtggcc cgcgtgtatc gggtcccctg
     1021 tcatattgtc tagcccgccc tgctgaaacc tccccccaca aatctcatct aaatcccgat
     1081 ccggtccgaa ggaccgtccg attgtgaaac ccatctggcg catcctgaca cacatgtgcg
     1141 ccaagcccct gccgggaacg tcgtccggcg tggaggaagc caatagcatg catccccgcc
     1201 tggtccagtt cgaggagccg gtggccggga tcgatccggc tgccccgagt gaagtcgtca
     1261 cgccgtccga cgccatcgaa gtcccgatca ttgacagcaa cctttcttcg gaaaccgaac
     1321 tcctcgatgc ctactcctcg gccgtcatcc gggtggccga aaaggtcagc ccggccgtca
     1381 tccggctgga tgtcctgcag cgcgtggagt cgaagcgcca ggggcaggcg gtggagcgtc
     1441 cgggttccgg ctcgggcttc ttcttcacgc cggacggttt cgcgctcacc aacagtcacg
     1501 tcgtccgcgg cgccacgaaa atcgaggcca tcctctccga cggacggcgc gttccggccc
     1561 gcctggtggg ggaggacccg gatacggacc ttgcggtggt gcgggtcacc ggcaacggtt
     1621 ttgcggcgct cacgctgggc gattcggagt cgctcaaggt cggccagatc gccatcgcga
     1681 tcggcaatcc gttcggcttc gactgtacgg tgacggccgg cgtggtgtcg gcactcgggc
     1741 gcagcctccg ttcccagtcg gggcgcctca tcgacgacgt cctgcagacc gacgcctccc
     1801 tcaatcccgg caactcgggc ggcccgctgg tcgattcgca cgggcgcgtc atcggcgtga
     1861 acacggccat catccggccg gcgcagggga tctgcttcgc ggtgtcggtc aacacggcgc
     1921 gcttcattgc ttcacggttg atgcgtgacg gcgtcgtgca ccgcggcaag ctcggcctcg
     1981 cggggcagta tgtggcgctc ccgcgctatt tcgtgcgttc actcggcctg tcgcgtccgg
     2041 gcggcgtgcg cgtgatgtcg gtggaacatg gcgggccggc cgacctggcg gggctcaagg
     2101 agggagatgt gatcgtgcac ctggccggcg aagccgtggc ggggattgac gaccttcacc
     2161 ggctcctgac cgacggacgg gtggaccagc cgttgccgat cacggtcgtc cgggccttcg
     2221 agaagatgga gttcgaggtg acgccggcgc gacgcgcggc ggcctagcgg ggcgtcggca
     2281 atcgccgatg tcgaagagca aatgacctga gttggatatg ggattgttga gcgggcgccc
     2341 ttcggggcgc ccgtcctgtt ttgtggacct gaagtcgtaa cagcgaacag gttccggaac
     2401 ctgttccgca aatgtctttc acattcaggc aagtgtggcc aaccgcagtt gggccgcgtg
     2461 cctggcccgg agtagaatcg ccacatccga ccggccaacg ccggcctcta cttcaggagg
     2521 atgcgatgac tcgattcgtg cccgccctcg ccctggccat tctgatcgcg ttggccatcg
     2581 ccccacccac ccgtgccgcc acgaccgaga ttccggtcgt ccgcattgaa aacacgacca
     2641 cgatcgcggc cgcgccggcc gccgtatgga catggctcac gaccggcaag aacctcatca
     2701 cctggtgccc ggtctggaaa agcgagtcca acaagtcggt ccatctcacg aaggtggggg
     2761 atgcgctcga cttcaccgac cagtggggaa acggggggcg ttccgttgtc accttcatcg
     2821 ccccgaacaa ggagctgcgg gtggcccacg agccgacgga cggctcgtac ctgtgccaat
     2881 cccgggtgat cctcaagccg gctggatctg gtaccgaagt gacctggatc gaacaatata
     2941 cggacgccag cagcgccacc gacaaggcgg ccacggccac gaagatggag accgagatgg
     3001 acgccacgtt gggtacactc aaggcgtccg tcgagacgaa atagccgccg atcggaggtg
     3061 tccttgcccg tccttcccac cgcctgcccc ctcgactgcc ccgaccactg cacgctggat
     3121 gtcaccgtcg aggaggggcg tgtcgtccgc gtgaacgacc gagcggagaa tccgatcacc
     3181 gcgggcttca tctgcggcaa ggtgcggcgc atcgaccggc acctctacgg aaaggaccgg
     3241 ctccgctacc cgggcgtgcg tgaagggaag aagggggagg ggcgtttccg ccgcgtgtcg
     3301 tgggatgagg cgctcggcat catcgccgac aagttcgggg ccatccgcgc gtcttcgggt
     3361 ggcgaggcca tcctgccgta ctactacggc ggctcgaacg gcgcgctcac gcaggacgcc
     3421 acggacgcca cgctcttccg ccgcctcggc accacgcgca tcctccgcac gctctgcgcc
     3481 gcggccaccg gccgggccac gcaggggctc tacggcaaga tgccgggcat ctcgtacctc
     3541 gacttcgaga aagccgaagt catcatcctg tggggccaga acccatcggc ctccagcatc
     3601 cacctcgtgc cgatcctgcg ccgggccatg gacaacggca ccaagctcat cgtggtcgac
     3661 ccgcgcgcca cgccgctggc caggctggca catcgtcacc tggcggtgcg ccccggaacc
     3721 gacctgccgc tggcgctcgc catcgcccgc tggctcttca cgaacggcag ggccgacgag
     3781 gcgttcctcg cgcagcacgt cagcggcgtc gaggctttcc gcgatcgcgc catgcgctgg
     3841 acactggaag aaacggcgcg cgtcaccggc ttggcggccg ccgacatcga ggccacggcc
     3901 gccgagtacg ccgccgccaa ccccgcgctc atccgctgcg gctggggcct cgagcggaac
     3961 cgcaacggcg gctccgcggt ggcgtcggtc atcgcgcttc ccgccatcgc cggcaagttc
     4021 ggcgtg
//
LOCUS       DASVOD010000662         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_407725, whole genome shotgun sequence.
ACCESSION   DASVOD010000662 DASVOD010000000
VERSION     DASVOD010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407725"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF720_14225"
     CDS             complement(<1..180)
                     /locus_tag="VF720_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018753353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX7880567.1"
                     /translation="MSPMSTTFPILLAFFLVFLNGFFVATEFALVKVRATRLAELAKK
                     GNRSAELAESLVHRLD"
     gene            complement(442..3303)
                     /locus_tag="VF720_14230"
     CDS             complement(442..3303)
                     /locus_tag="VF720_14230"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX7880568.1"
                     /translation="MAKKPAKSIDARPVGTATPAIRSEAAGRRPAPRATRSPNGSGSG
                     HHGLRFDRRNSRPDVNPFEELEWDLRTAAISSEKGELIFEQKDVEIPKNWSMMATNVV
                     VSKYFRGNLGTPEREKSVRQLIGRVVNTITRWGSKDGYFASDEDQAAFRDELAHLLLH
                     QKMAFNSPVWFNCGVEDKPQCSACFINSVDDTMASIMDLAKTEGMLFKWGSGTGTNLS
                     PIRSSREKLKGGGTASGPVSFMKGYDAFAGVIKSGGKTRRAAKMVILNADHPDIVDFI
                     RCKELEEKKAWALIDAGYESSFNGEAYASVFFQNSNNSVRVTDEFMDAVEKDKEWRTR
                     AVTSREVMDTYKARDIMGMITESAWLCGDPGLQFDTTINRWHTCPNTAPINASNPCSE
                     YMFLDDSACNLASLNLMKFRRADGEFDAEAFQHAVDISITAQEIIVANASYPTPAIEK
                     NSHDYRPLGLGYANLGALLMARGVPYDSDAGRAVAGAITSLMHGQAYATSARIAAQLG
                     PFAGYERNREPMLGVIDMHKGSVQGIDPTLVAGDLMGAVRETWARARTLGGEYGYRNS
                     QVTVLAPTGTIGFMMDCDTTGVEPEIAIVKYKKLVGGGMLKIVNQTVPEALSRLGYSS
                     IEIKKIVEYINENETIEGAPGLRDEHLAVFDCAFRPAKGSRSIHYRGHIRMMAAVQPF
                     LSGAISKTVNMPGEATREEIAETYLEAWKLGLKAIAIYRDGCKRSQPVNTGLDKDDAA
                     AAKLFPVGPHRRRLPDERRSITHKYSVGGHEGYITVGMYDDGSPGEIFLNMAKEGSVV
                     SGLMDSFATAISLSLQYGVPIRDLVNKFSHVRYEPSGYTRNPEIPLAKSITDYIFRWL
                     ASRFLSPEEAIDLGVQKAITDNGLTAPDTSSTERVINLAGPVVQSAPTAGRARGADDP
                     SLTFDNQSDAPPCSECGSTLIRSGACYKCVNCGATAGCS"
     gene            3835..>4237
                     /locus_tag="VF720_14235"
     CDS             3835..>4237
                     /locus_tag="VF720_14235"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX7880569.1"
                     /translation="MHHFAATTLAAVLVLAAAPALAHGSEGHSHAPAATKPETAKAEE
                     PDTLPIGKAVPDFTLTSADGKSIDLATYGKGSVTVLTFLSKNCPVSRAWHKEIASIAK
                     EYDGKVKFLGIMSNSTEKTPEVATFLKGEGIA"
BASE COUNT          688 a         1340 c         1456 g          753 t
ORIGIN      
        1 atccaggcgg tgcaccaggg attcggccag ctcggccgag cggttcccct ttttcgccag
       61 ctccgccagt ctcgtggccc gcaccttgac gagggcaaac tcggtggcca cgaaaaagcc
      121 gttgaggaag acgaggaaga aggcgaggag gatggggaag gtggtggaca tgggcgacat
      181 attgcccgcg cgggctcagg ctgacaatga gggccgggga attcccgctt gcctacggaa
      241 gacttccccc atcccagtcg tgcttcggat ccggaaggaa gatgcggcca aggccggtag
      301 gcgcaggcgg actcgagtgc cccatgagga tgcaacaaac gaaggaagcg gaaagcccgt
      361 cggccccgcc ccggtcacga aggaccggga cagggccggc gagggtgttg ccagatcagg
      421 tcaatgactc aggtcgggct ctcaggaaca tcccgccgtc gcgccgcagt tcacgcactt
      481 gtagcaggcg ccgctgcgga tcagcgtgga gccgcattcg gagcaggggg gtgcgtcgga
      541 ctggttgtcg aaggtgaggc tggggtcgtc ggctccgcgg gcacgaccgg cggtcggcgc
      601 ggactggacc accgggccgg ccaggttgat gacccgttcg gtggacgagg tgtcgggcgc
      661 ggtgaggccg ttgtccgtga tcgccttctg gacgccgagg tcgatggctt cctccggcga
      721 gaggaaacgg gaggcgagcc agcggaagat gtaatcggtg atcgacttgg cgagcgggat
      781 ttccgggttg cgcgtgtagc cgctcggctc gtaacgcaca tggctgaact tgttcaccag
      841 gtcgcggatc ggcaccccgt actgcaggga gaggctgatg gcggtggcga acgagtccat
      901 gaggccggac acgaccgagc cttccttggc catgttcagg aagatctcgc cggggctgcc
      961 gtcgtcgtac atgccgacgg tgatgtaccc ctcgtgtcca cccacggagt acttgtgggt
     1021 gatcgagcgg cgttcgtcgg gaaggcggcg gcgatgcgga ccgaccggga agagcttcgc
     1081 ggcggcggcg tcatccttgt cgaggccggt gttcacgggc tgtgagcgct tgcaaccgtc
     1141 gcggtagatg gcgatggcct tgaggccgag cttccaggcc tcgaggtacg tctcggcgat
     1201 ctcctcgcgg gtggcttctc ccggcatgtt gacggtcttg gagatggcgc ccgacaggaa
     1261 gggctgcacc gccgccatca tccggatgtg gccccggtaa tggatgctgc gcgacccctt
     1321 cgccggccgg aaggcgcaat cgaacaccgc gaggtgttcg tcacgcagcc cgggcgcacc
     1381 ctcgatcgtc tcgttctcgt tgatgtactc gacgatcttc ttgatctcga tcgaggagta
     1441 gccgagccgc gaaagagcct ccggaaccgt ctggttgacg attttgagca tgccgccgcc
     1501 gaccagcttc ttgtacttca cgatggcgat ctccggctcc acgccggtcg tgtcgcaatc
     1561 catcatgaag ccgatggtgc cggtcggggc cagcaccgtc acctggctgt tgcggtagcc
     1621 gtactcgccg cccagcgtgc gggcgcgggc ccacgtctcg cggacggcgc ccatcaggtc
     1681 gcctgccacc agcgtgggat cgatcccctg gacggaaccc ttgtgcatgt cgatgacgcc
     1741 gagcatcggc tcgcgattgc gctcgtagcc ggcgaacggc cccagctgcg cggcgatgcg
     1801 cgcgctggta gcgtaggcct gcccgtgcat cagcgaggtg atggctccgg caaccgcgcg
     1861 cccggcatcc gagtcgtacg gcacgccgcg ggccatgagg agcgcgccca ggttggcgta
     1921 gccgaggccg agcggccggt agtcgtggct gttcttctcg atggccggcg tcgggtagct
     1981 cgcgttggcc acgatgattt cctgggcggt gatggagatg tccaccgcgt gctggaacgc
     2041 ctcggcgtcg aactcgccgt cggcgcgccg gaacttcatg aggttgagcg aagccaggtt
     2101 gcatgccgag tcgtcgagga acatgtattc gctgcaggga ttgctggcgt tgatcggcgc
     2161 cgtgttgggg caggtgtgcc accggttgat ggtggtgtcg aactggaggc cgggatcgcc
     2221 gcacagccag gccgattcgg tgatcatccc catgatgtcg cgcgccttgt aggtgtccat
     2281 cacctcgcgg gaggtgacgg cccgggtgcg ccactccttg tccttttcca ccgcgtccat
     2341 gaactcgtcg gtaacgcgca ccgagttgtt ggagttctgg aagaagaccg acgcgtacgc
     2401 ctcgccgttg aaggacgatt cgtagccggc atcgatgagg gcccaggcct ttttctcctc
     2461 gagttccttg cagcggatga aatcgacgat gtcggggtgg tcggcgttca ggatgaccat
     2521 cttggccgcc cgccgtgtct tgccgccgga cttgatgacg ccggcgaagg cgtcgtagcc
     2581 cttcatgaac gacaccggcc cggaggcggt gccgccgccc ttcaacttct cgcggctcga
     2641 acggatgggg gagaggttcg tcccggtgcc ggaaccccac ttgaacagca ttccctcggt
     2701 cttggccagg tccatgatgg aagccatggt gtcgtcgacc gaattgatga agcaggccga
     2761 gcactgcggc ttgtcttcga cgccgcagtt gaaccagacc gggctgttga acgccatctt
     2821 ctggtggagg agcagatggg ccagttcgtc gcggaaggcc gcctggtcct cgtcggaggc
     2881 aaagtagccg tccttggagc cccagcgcgt gatcgtgttg acgacgcggc cgatcaactg
     2941 gcgcacgctc ttctcgcgtt cgggcgtccc caggttgccg cggaaatact tcgagacgac
     3001 cacgttggtg gccatcatcg accagttctt cgggatctcc acgtccttct gttcgaagat
     3061 cagttcaccc ttctcacttg agatcgcggc cgtgcgcagg tcccattcga gttcctcgaa
     3121 cgggtttacg tccgggcggc tgttccgccg gtcgaaacgg agcccgtggt gtccgctgcc
     3181 ggaaccgttc gggctccggg tggcgcgggg agcgggacgg cggccggcgg cctcgctccg
     3241 gatggccggg gtggccgtac ccactggacg ggcgtcgatc gacttggccg gtttcttcgc
     3301 catgggaaat cgtcttcctc ctcccgactt tcctccgggc gggggggtgc caggcacccc
     3361 cagggattcc gcccgctggc agcccaatca gggtccgact gccagaccat cgaggcccga
     3421 cccccgccgg tcctcgtcct ccaactccat accctggtcg cccgtgacgc cggtgattca
     3481 cgtccggcga ggtgcggcac ggtttttact cccgccggac ctgccagtca agcaaaaacg
     3541 caacattctg tggacgctcc ggtgacgacc tactagatat ggtggtacgg gggaagggtg
     3601 gctgtccact aatttgtcat cggaacgggc ggcaggcggc cgatcgggcc cgaaagtccg
     3661 ctcgtgcccc gggctggcgg ccggcgaacc ggcgggccgc cccggtgttc tgaacgcctc
     3721 gcgcccccga tccgtcaggg acggggacct gccgggcctg gacatggcac actggcgggc
     3781 gccgcgaccg gacctgcatc cgacccagac gatcgaaccc gaggagaccc atccatgcat
     3841 catttcgccg ccacgaccct ggccgccgtg ctcgtcctgg ccgccgcccc cgcccttgcc
     3901 catggctccg aagggcactc tcacgccccg gctgcgacga agcccgaaac cgcgaaggcg
     3961 gaggagcccg atacgcttcc gatcggaaag gcggtccccg acttcacgct gaccagcgcc
     4021 gacggcaaga gcatcgacct ggcgacgtac ggcaagggat cggtgaccgt cctcacgttc
     4081 ctctcgaaga actgccccgt gtcgcgcgcc tggcacaagg agatcgccag catcgcgaag
     4141 gagtatgacg gcaaggtgaa gttcctcggg atcatgtcga actccaccga gaagaccccc
     4201 gaggtggcga cgttcctgaa gggcgaaggg atcgcct
//
LOCUS       DASVOD010000663         2430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_97304, whole genome shotgun sequence.
ACCESSION   DASVOD010000663 DASVOD010000000
VERSION     DASVOD010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97304"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..695
                     /locus_tag="VF720_14240"
     CDS             393..695
                     /locus_tag="VF720_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002050969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX7880570.1"
                     /translation="MVKVALFVRLEAKAGMEKDVEQFLLGGLPLVQEEPATTAWFGIR
                     LGPSTFGIFDAFPDEAGREAHLAGKVAAALMAKAGELFSTPPSIEKVDVLAAKLPG"
     gene            767..2404
                     /locus_tag="VF720_14245"
     CDS             767..2404
                     /locus_tag="VF720_14245"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7880571.1"
                     /translation="MIDQDLRAAAFPKLDKTQLAALEGCPLSKLKHCRDGEKLFEAAQ
                     RDSNFYVVKTGAVEIVDDDGDESKVVATLGPGEFTGEVGQLTGGPAIFSGIVRGESDV
                     FEISNDALREIMNVHPDLGDVILRAFMARRHLLHEMGDFTGLRVIGSRYSRETLRVRE
                     FLAKNLVPFTWMDLEDDPQVRQFLEQAGLTEADTPVVTWGHKLLLRNPSNQELADALA
                     LRRPLEHTVYDLAVVGAGPAGLAAAVYGASEGLNTVVLERDAPGGQAGRSMRIENYLG
                     FPTGITGRELAENAAVQANKFGAHLPVASPVTGLSFDNKYAVLHFHNGETVSAKCLLI
                     ATGADYRLLGVEGCERFEGCGVYYAATPVEAQLCKGSDVVVVGGGNSAGQATVFLSGQ
                     VRKVCLVVRGSDLYEHMSSYLAKRTEETPNVEVLLDTEVRRMSGTAASARPRSSTTGR
                     ARRGRSGPPPCSASSARRRGPTGSLRKSRRTRKDSCGPAHPWRVPRRGRHVGSPTCWR
                     PAAPASSPLATSGPAQPSASPRPSARGRWRSCSCTHI"
BASE COUNT          445 a          765 c          811 g          409 t
ORIGIN      
        1 cattgacttt gtcagtgctc gggagcctaa tgctgattga ccctcccttc gacgccacct
       61 ccataggcgc caagagacag gccgagcccc tggccgttga ttcaagctgc aaacacccat
      121 gtgctgtgaa acggagtcat ccgaagactg ccgcatggaa cccaccgaga gatagccgtc
      181 gtgacggtcg atttctcaac gcaccgggga ctggtgtatc ggcgaacgga gaggttggcg
      241 acggcaagtg ggggtcctcc cgatttgaat gccgcctgat caatgccacg cttgccggag
      301 tcgtttgacc tgctcatcgc caggctggac gttacgaacc gcggggatgc acaagcgatc
      361 gtgggtgcgt ccataaccaa aggaggtgcg tcatggtcaa agtagcgtta ttcgtccgtt
      421 tggaagcgaa ggccgggatg gagaaggatg ttgagcaatt cctgctcggt ggtcttccac
      481 tcgttcagga ggagccggcc accaccgcgt ggttcggcat tcgccttgga ccatccacgt
      541 tcgggatctt tgatgccttt ccagatgagg cgggccgtga ggcgcacctt gccggaaagg
      601 tcgctgccgc tctgatggcg aaggcgggag agcttttctc cacgccgccg tccatcgaga
      661 aagttgacgt tctggcggca aagctaccgg ggtagtgaag ccctaaaagg tgttacagcc
      721 gtggcgtgac tgaagccgac agcgtcggag caaaggagcc cgggacatga tcgatcagga
      781 cctgcgcgcc gccgcattcc ccaagctcga caagacccag ctcgccgcgc tggaaggttg
      841 ccctctgtcg aagctcaagc attgtcgcga cggggagaag ctcttcgaag ccgcgcaacg
      901 cgattccaac ttctacgtcg tcaagaccgg cgcggtcgag atcgtcgatg acgacggcga
      961 tgagtcaaag gtcgtcgcga ccctggggcc cggggaattc accggcgagg tgggccagtt
     1021 gaccgggggc ccggcgatct tcagcggcat cgtccggggc gagagcgacg tcttcgagat
     1081 ctcgaacgat gccctgcggg agatcatgaa tgtccacccg gacctgggcg acgtcatcct
     1141 ccgggccttc atggcccgac gccacctcct ccacgagatg ggggacttca cgggcctgcg
     1201 cgtgatcgga tcgcgctatt cgagggaaac cctccgggtc cgcgagttcc tggccaagaa
     1261 cctggtgccg ttcacctgga tggacctgga agacgatccg caggtgaggc aatttctcga
     1321 gcaggccggg ctgaccgagg ccgacacgcc cgtggtcacc tggggccaca agctgctcct
     1381 gcgcaacccc tcgaaccagg agctggcgga tgccctggcc ctccgccggc cgctcgagca
     1441 cacggtgtat gacctggccg tggtgggagc cggcccggcg ggactggccg cggccgtcta
     1501 tggcgcctcc gaggggctga acacggtcgt gctggagcgg gacgcgccag ggggtcaggc
     1561 cggtcgcagc atgcggatcg agaactacct cggcttcccc accggcatca cgggccgcga
     1621 acttgccgag aacgccgccg tgcaggccaa caaattcggg gcgcacctcc ccgtcgcatc
     1681 gccggtcacg ggcctgtcgt tcgacaacaa gtacgcggtg ctgcacttcc acaacggcga
     1741 gacggtttcg gccaagtgcc tgttgatcgc caccggggcc gactatcgcc ttctgggcgt
     1801 cgaagggtgc gaacggttcg aagggtgcgg cgtctactac gccgccaccc ccgtcgaggc
     1861 gcagctctgc aagggttcgg acgtcgtggt cgtcggcggc ggcaactccg ccggccaggc
     1921 caccgtcttc ctctccgggc aggtccgcaa ggtgtgcctg gtggtgcgcg gcagcgacct
     1981 ctacgaacac atgtcgagct acctcgcgaa gcggaccgag gaaaccccca acgtcgaagt
     2041 gctcctcgat accgaggtcc ggcggatgtc ggggaccgct gcctccgcga ggccgaggtc
     2101 gtcaacaaca ggacgggcga ggcgcggacg atcaggacca ccgccctgtt cagcttcatc
     2161 ggcgcgacgc cgcggaccga ctggctccct caggaaatcg agaaggacga gaaaggattc
     2221 gtgcggaccg gcgcatccct ggagagttcc gaggcgtgga cggcacgtcg gaagccccac
     2281 ctgctggaga ccagccgccc cggcgtcttc gccgctggcg acgtccggtc cggctcagcc
     2341 aagcgcgtcg cctcggccgt cggcgagggg tcgatggcgg tcatgttcgt gcacgcatat
     2401 ctgaaggaga tgtgaatcag tctgggcgaa
//
LOCUS       DASVOD010000664         5906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_47196, whole genome shotgun sequence.
ACCESSION   DASVOD010000664 DASVOD010000000
VERSION     DASVOD010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47196"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            492..710
                     /locus_tag="VF720_14250"
     CDS             492..710
                     /locus_tag="VF720_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018629121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HEX7880572.1"
                     /translation="MPAKKKASSSRRYGPKATDKVEKVMHERKHGTLRSSSGAKVTSR
                     KQAIAIGLSEARRAGAKVPAKKRSSSSR"
     gene            complement(730..1578)
                     /locus_tag="VF720_14255"
     CDS             complement(730..1578)
                     /locus_tag="VF720_14255"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX7880573.1"
                     /translation="MKIVPVLLLAALLSAPAAAQVKLPQPSPAASVSRAVGITDVRID
                     YHSPGVKKREIFGGLVPYNEVWRLGANDATTLRVTSPVKIGEHDLATGIYALFAIPGE
                     KNWTIIVNKDWQQWGSYFYKPENDLFRFDVPAHRDELVEWMRFDIETADPNTLKVSFA
                     WDRTRWSFDVTADVNRLVWAQLDSAMAVDGPEKWEACHQAARFSLDTGERSDQAMTWI
                     DQAMALKQSFWNDELKARLLHRAGKTAEALPLLDSAMTLAEGKAPKEYIDGLVKTRAE
                     WTAGAP"
     gene            1689..2543
                     /locus_tag="VF720_14260"
     CDS             1689..2543
                     /locus_tag="VF720_14260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7880574.1"
                     /translation="MSRFDLYDHPRWYDILFARGSAAKIRGLVRVARDWTESARHPAW
                     LEPACGTGRLLVPAARRGISVTGYDRSAAMVEFAARQLAKFGRKARVVEADLVDAHRQ
                     LGTARFDLAFTLDNSLRHLPDDGAVAAHLASTARVLKPGGAYVVGLSFHDPGRADTSE
                     DIWTGQRGKTRVTQMVQFEPPPAGSRFRHETVIEHVEVSDAAGAHHHDAVWRLLLLEQ
                     ARWERLVGDSPFTWVATLDATGSPIEPGVFDYQWLVLTPRRRSTSGQAASRHAGVSRP
                     IRRPAVAR"
     gene            complement(2438..2971)
                     /locus_tag="VF720_14265"
     CDS             complement(2438..2971)
                     /locus_tag="VF720_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880575.1"
                     /translation="MTSHEWNARRILKELCTAERRKEILTLFWTDAEKDTRELVTAAL
                     AKGLNFRLDSVRKATAARRAELLGMQLHVPQFEEPLEIALMLYHTEKASDLLAAFLDA
                     WKIPHVNGSIEVEEYPVPDADAVARAVQKLKSTYAEPDIALYLATAGLLMGRETPAWR
                     DAAWPEVDRLRGVNTSH"
     gene            complement(3089..4228)
                     /locus_tag="VF720_14270"
     CDS             complement(3089..4228)
                     /locus_tag="VF720_14270"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7880576.1"
                     /translation="MSYADFVRAAAAVRTEAAFRALPFFRTHGAALDALLVTPRPVGD
                     AARSSAPSASPPRTLSVVHWNIEKGKELDRLRRRLRDEPRLRDADAWCLNEVDDGMAR
                     TGNRDIAAELAEVLGAHGFYLPTYIECTKGLPAERLVTGENRLGLHGIAVLSRRPIRE
                     VGVLQLPPAWDYFDYSEKRYGGRRALVVRIDAGFGDADLIATHLEMRAPPAGRTTQVR
                     ALVNGYAAWADARPPAPALAAGDFNTHTFSRGRSGDTLRGFIRVVGTQNDRLCRELLE
                     PAGREDLFRELERGGWRLAGFNDTAPTAWQELGNVEDLKMLPPALGEFVLRAFRLEGR
                     RLPLRLDWFAAKGLAPLEAATIDSIVPGDGPASDHEPIWVRVGPA"
     gene            complement(4360..>5906)
                     /locus_tag="VF720_14275"
     CDS             complement(4360..>5906)
                     /locus_tag="VF720_14275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880577.1"
                     /translation="SDGLQLCNAPGDQTRPQGISLSNGDVVLAWSDMRSDAGDIYLTR
                     IGANLPNLSVADAGFGFDLPLVPRNRNDATPSQARLSPVLDGGQATTRLNWSVTLEGS
                     NVIAGWGLDVTLDGLPLVSVPDMGPSAPGTVIALNQGPWTVRGGRHTLTLRADPAGAV
                     REATESDNINSCQFVWSPEALVPGTFTDRPAPPARGTFTEPNSHGYVIHPGASAWGVA
                     VGAGELGKDVDLIAYDDYSGSTSGLSHRVAASGGGPGRVDWVVGSAAFAPSIYPAVTA
                     GAEKGSQGNYVMTSVDATGRRGTSDTGRWAAQPMAANKLLDVYDLDLVAGRAYHLTLV
                     RALGRAPVEFYVLPPAAGAYGPDDAIATSTHLTADGEGVGVLTFVPTVTGRYPVAVAR
                     TTTEWLDEGITYHFFYSTQVSGVGDVDAAPRAMVLSAPWPNPTASATRVTLSLPEAGP
                     VDVRLFDVHGRLVATLLEATLPAGAHAIAWDGRDAAGTPVPAGLYFVRSTALGDVRTQ
                     RVVVRH"
BASE COUNT          937 a         1908 c         2110 g          951 t
ORIGIN      
        1 gaggggatca cctccaccgt gatgaggtga tcgtcaaacc acgcttcggt gggccccggg
       61 ggcaggctgg cggccaagga cgcgcccggg acgagcgcga cgagcaatga cgcgatcctg
      121 gcaaggcgtg tcatggcgcc gatctccgtt caatgaaggc cggacaatcg gagcgattcc
      181 gacgcccgcg cttctctgca ttcaaacgaa gccagctgca tagggtgtgc cgtggccggg
      241 tcgccggaag aatcttcccg gcatcacggg ccggcgaatg cgccattttg ccgtggcccg
      301 gagcacggaa atttcgtccg attccaccgt gtcgcgatgg ggcgggaacg aacttccgag
      361 gttgcgtcag gacgggaaat atccgactga atcgggaggc ctgtgcgacg cggcacattc
      421 tgtgcaggtt tgcacgaacg gaatccgtgg ccgcgcacgt tcaactttac gcaaccggaa
      481 gaggtaacga gatgcccgcc aaaaagaaag cgtcatcctc gcgacgctac ggaccgaagg
      541 ccaccgacaa ggtggagaag gtcatgcatg aacggaagca cggcacgctg cgctcgagct
      601 cgggcgccaa ggtcacgagc cggaagcagg ccattgccat cggcctgtcc gaggcacggc
      661 gcgccggggc caaggtgccg gcgaagaaac gaagcagcag cagtcggtaa cggagggagg
      721 cgcggggcgc tatggcgctc cggccgtcca ttcggccctg gttttgacca ggccgtcgat
      781 gtactccttc ggcgccttcc cctcggcgag cgtcatggcc gagtcgagga gcgggagcgc
      841 ttcggcggtc ttgccggcgc gatggagcag gcgggccttg agctcatcgt tccagaagct
      901 ctgcttcagg gccatggcct ggtcgatcca ggtcatggcc tggtcgctcc gttcgccggt
      961 atcgaggctg aagcgggcgg cctggtggca ggcttcccac ttttcgggtc catccaccgc
     1021 catggccgaa tcgagctggg cccagacgag gcggttcacg tcggcggtca cgtcgaacga
     1081 ccagcgcgtg cggtcccagg caaagctcac tttgagcgtg ttagggtcgg cggtttcgat
     1141 gtcgaacctc atccactcga cgagttcgtc gcgatgggcc ggcacatcga agcgaaacag
     1201 gtcgttttcg ggcttgtaga agtacgaccc ccactgctgc caatccttgt tcacgatgat
     1261 ggtccagttc ttctcgcccg ggatggcaaa aagcgcgtag atgccggtag ccaggtcgtg
     1321 ctcaccgatc ttgaccgggc tcgtgacgcg gagcgtggtg gcgtcgttgg cgccgagccg
     1381 ccacacctcg ttgtacggaa ccaagccgcc gaagatctcc cgttttttca caccgggaga
     1441 gtgataatcg atccggacat cggtgatgcc cacggcgcgc gatacggagg cggccggact
     1501 gggctggggc aacttgacct gggcggcggc cggggcactc aagagtgccg ccagcaacag
     1561 aacggggacg atcttcatcg gtttctccat cggccggggt ccgggggttt catgggtgat
     1621 cttacccgag gtccgggagc catgaggagt ggattcgaga ggccccatca ctcgataatg
     1681 ccgggccgat gagccgcttc gacctctacg accatccgcg ctggtacgac atcctgttcg
     1741 ccaggggcag cgcggccaag atccgcgggc tcgtgcgcgt ggcgcgtgac tggactgagt
     1801 ccgcacgcca cccggcctgg ctggagccgg cctgcggcac cggccggctt ctcgtgccgg
     1861 cggcgcggcg cggcatttcc gtgaccggtt acgaccgcag cgcggcgatg gtggagttcg
     1921 cggcccggca gttggccaaa ttcggacgga aggcccgtgt cgtcgaagcc gacctggtgg
     1981 atgcgcaccg tcagttgggt acggcgcgtt tcgatctcgc cttcacgctc gataactcgc
     2041 ttcgtcacct gccggatgac ggggccgtgg cggcacacct ggcctcgacc gcgcgggtgt
     2101 tgaaaccggg cggcgcctac gtcgtgggac tctcgttcca cgatcccggc cgggcggaca
     2161 cgagtgaaga catctggacc gggcagcgcg gaaagacgcg cgtcacgcag atggtgcagt
     2221 tcgagccgcc gcccgccggc tcgcgttttc gacatgagac cgtgatcgag cacgtggagg
     2281 tttccgacgc cgccggtgcg catcatcacg acgccgtgtg gcggctcctg ctgctcgagc
     2341 aggcgcgctg ggaacgcctg gtcggcgact ccccgtttac ctgggtggcc acgctggatg
     2401 ccaccgggtc gccgatcgag ccgggcgttt tcgattatca gtggctggtg ttgacgcccc
     2461 gcaggcgatc cacctcgggc caggcggcat cgcgccacgc aggcgtctcg cgccccatca
     2521 ggaggccggc cgtcgccagg tagagggcga tgtcgggctc ggcatacgtc gacttcagct
     2581 tctgcaccgc cctggccacc gcgtcggcat ccggcaccgg gtactcctcc acctcgatcg
     2641 acccgttcac gtggggaatc ttccaggcat caaggaaggc ggcgagcagg tcgctggcct
     2701 tctcggtgtg atagagcatc agcgcgatct cgagcggctc ctcgaactgc ggcacgtgga
     2761 gctgcatgcc gagcagttcg gcccggcgtg cggctgtggc cttgcgcacc gagtcgaggc
     2821 ggaagttgag acccttggcc agcgcggcgg tcaccagttc acgggtgtcc ttctccgcgt
     2881 cggtccagaa cagggtaagg atttccttgc gccgctcggc ggtgcacagc tctttcagga
     2941 tgcgtcgggc gttccactcg tggctcgtca acggggtcgt gcctccaggt atcagggtgt
     3001 cgggggggtg tcgtgcgcgt aaccagagag aaggtacccg accgaagggt gatggtcgag
     3061 gtgccggatc gcttggcgaa ccagtgcctc acgccggccc cacgcgcacc cagatcggtt
     3121 catggtctga ggccgggccg tcccccggca cgatggagtc gatcgtggcc gcctccagcg
     3181 gcgcgagacc cttcgcggcg aaccagtcga ggcggagcgg aaggcgtcgc ccctcgagcc
     3241 ggaaggcgcg cagaacgaac tccccgagcg cgggcggcag cattttcagg tcttccacgt
     3301 ttcccaattc ctgccaggcg gtcggtgccg tgtcgttgaa gccggcgagg cgccaccctc
     3361 cccgttccag ttcgcggaag aggtcctcgc gtccggcggg ttcgaggagt tcgcggcaga
     3421 ggcggtcgtt ctgcgtgccg accacgcgga tgaatccccg gagcgtgtct ccggatcttc
     3481 cacgcgaaaa cgtgtgcgtg ttgaaatcgc ccgcggccag cgcgggcgcc ggtggacgcg
     3541 cgtcggccca ggccgcgtag ccgtttacca gcgcacggac ctgcgtggtg cgccccgcgg
     3601 gcggggcgcg catctcgagg tgtgttgcga tcaggtcggc gtcaccgaaa ccggcgtcga
     3661 tccggaccac gagcgcgcga cgtcctccgt atcgtttttc gctgtagtcg aagtagtccc
     3721 acgcgggggg gagttgaagc acgcccacct cgcggatcgg ccgcctggag aggactgcta
     3781 tcccgtgaag gccaaggcgg ttttcgcccg tcacgagtcg ctcggccggt aaacccttcg
     3841 tgcactcgat gtaggtcggc agatagaagc cgtgggcgcc gagaacctcg gccagttcgg
     3901 cggcgatgtc gcggttgccg gtgcgcgcca tgccgtcgtc cacctcgttc aggcaccagg
     3961 catcggcgtc acggaggcgc ggctcgtcac ggaggcgccg gcggagccgg tcgagctcct
     4021 ttcccttttc gatattccag tgaacgacgc tcagcgtgcg gggcggtgat gcggatggag
     4081 cgctggaccg ggcggcatcc cccaccggtc gcggcgtgac caggagcgcg tcgagcgcgg
     4141 caccgtgcgt ccggaagaac ggcaaggccc ggaacgcggc ctcggtgcga acggccgccg
     4201 cggccctcac gaaatcggcg taggacacgc cggtgaccag cgtcgaaggg gatgtgtcgt
     4261 ttcggatgtt cgtgtcgctg gcgatgatcg gcctccctgc ccatacgggc ggcgccgatc
     4321 atccccgatc aggcgggccg gctcaacgcc gggccccggt caatgccgga caacgacccg
     4381 ctgcgtccgc acgtcaccga gcgcggtgga tcgcacgaag tagaggccgg cgggcaccgg
     4441 tgtgccggcg gcatcgcgcc cgtcccaggc gatggcgtgc gcaccggccg ggagggtggc
     4501 ttcgagaagc gtggcgacca gccggccgtg gacgtcgaac agccggacat ccaccggacc
     4561 ggcctcggga agggagagcg tgacccgcgt ggccgaggcg gttggattgg gccacggcgc
     4621 ggagaggacc atggcgcgcg gcgcggcatc gacatcgccg acacccgaca cctgggtgct
     4681 gtagaagaag tggtaggtga tcccctcgtc gagccattcg gtggtggtgc gggccacggc
     4741 cacgggatag cggccggtga ccgtcggcac gaaggtaagg acgccgactc cctcgccgtc
     4801 cgccgtcagg tgcgtggagg tggcgatggc gtcgtcgggg ccgtaggcgc ccgccgcggg
     4861 tgggagcacg tagaactcga ccggcgcacg tccgagcgcg cgcaccagcg tgaggtgata
     4921 ggcgcgaccc gccacgaggt cgaggtcgta cacgtcgagc agcttgttgg cggccatcgg
     4981 ctgcgcggcc caccgcccgg tgtcggaggt gccacggcgc ccggtggcat cgacactggt
     5041 catgacatag ttcccctggc tccccttctc ggcccccgcc gtgacggccg ggtagatcga
     5101 gggggcaaag gcggccgatc cgaccaccca atcgacgcga cccgggccgc cgccggatgc
     5161 ggccacgcga tgactgaggc cgctggtgga accgctgtaa tcgtcatacg caatcaggtc
     5221 gacatccttt cccagttccc cggcccccac cgccacgccc caggccgagg cgccgggatg
     5281 gatcacgtag ccatggctgt tcggctcggt gaaggtgccg cgcgccggcg gagccgggcg
     5341 gtcggtgaag gtgccgggca ccagcgcctc ggggctccag acaaactggc aggagttgat
     5401 gttgtcgctc tcggtggctt cgcggacggc gccggcggga tcggcccgca gcgtcagcgt
     5461 gtggcggccg ccgcgcaccg tccacggccc ctggttcagg gcgatcacgg tgccgggcgc
     5521 cgaggggccc atatcgggca cgctcacgag cggcaagccg tcgagcgtca cgtcgagacc
     5581 ccagccggcg atcacgttgc tcccttcgag cgtcaccgac cagttgaggc gcgtcgtggc
     5641 ctggccgccg tcgagtaccg gcgagagtcg agcctgcgaa ggagtggcgt cattccggtt
     5701 gcgcggcacc agcggaaggt cgaagccgaa accggcgtcg gccaccgaga ggttcgggag
     5761 gttggcgccg atccgcgtca ggtagatgtc gccggcatcg ctccgcatgt cggaccaggc
     5821 caggaccacg tccccgttcg aaagggagat tccctgcggc cgcgtctggt cgccgggggc
     5881 gttgcagagt tgcagtccgt cactgg
//
LOCUS       DASVOD010000665         4972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_929734, whole genome shotgun sequence.
ACCESSION   DASVOD010000665 DASVOD010000000
VERSION     DASVOD010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929734"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..4959)
                     /locus_tag="VF720_14280"
     CDS             complement(<1..4959)
                     /locus_tag="VF720_14280"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX7880578.1"
                     /translation="MSTATITPTTTDIIETATAPQRHRDILSPEGLEAHARLLAGRFA
                     VGESTGEAGHVFHERLNAGARILRDAYRKLIADAAGAESPLPAAEWLLDNFHLVEAEL
                     ADIRRNMPKRFIDRLPVLTGGDHAGRPRIQALAIELLRHSDASIDLARLIRYITAFQT
                     LVPLTIGELWAWPTLLKAELIENIRRLAEQLMVSRDARLTADRFIHRFEAAGEDEPMP
                     PLPDPLPAASVAQLLERFREFGPRVSPLRVQLSERLAARGLSPEEAIRAEHVAQAAAQ
                     TSMANAITGLRLCSTLDWNRNFERVSLVEQALRRDPSGVYARMDFASRNRYREAIEAL
                     AGPTGESEIGVALKAVELARRGAQSAPHDVRVGHVGHYILGGGRPQLEAASEHPARGI
                     RSLRRAVFSAAAPLYIGSILLLTGFLVWLALRLIPGGGFDPLITGLLILPASDLAIHI
                     VQWLVPLVARPHRLPRLDLESGVPEEGRTMVIVPTLLTSVEGVRHLLDNLEVQALAND
                     DPRIHFALVTDFADAPSGEMPDDPVILEAARAGIRELNARHGPARTDQFHLLHRGRRW
                     NPIEKVWMGWERKRGKIEEFNRYIRGDSTTGFEVHVGDPTVLSKVRYCITLDSDTRLP
                     RGVARQLIGIALHPLHRPVFDPKSGRVASGYGILQPRVSVTMSSVAGSLFSRVYAGHT
                     GVDPYTTAVSDVYQDLFCEGIFTGKGLYDVDALMSALEGRVPPNLILSHDLFEGLHAR
                     TGLVSDLEVVDDYPSSVLVHTRRQHRWVRGDWQILLWLLPWVPKLGGLAENRLPLISR
                     WKIFDNLRRSLVAPSLLVLLAFAWTVLPGSPLSWTLGVLLAMAFPVYRPIAQALHGPG
                     PGQSWRHFTREIGHELSTALAQTLMAITFLAYHAYRMTHAVILTLARLLITRQRLLEW
                     ETAAFVSRLLEGRGLTLYLAEMGMSPIMALFLTSIIIPLRPHALPVAAPFLLLWTLAP
                     LLAWRWSRPTRIKEFDPSPEERAWLERIAARTWDYFKQYMTADNHWLPPDNLQEIPTE
                     MLARRTSPTNIGLGLLSTLAAHDLGFLDEAGLVERLEGAMDTIEGLEQHEGHLLNWYS
                     TATLAPLHPRYVSTVDSGNLAGALVALAVGLREVAADSTRDAPGDGSTGVARRRRLLD
                     LAERCEARMQLMDFRFLFDRERKLFTIGYRLADREGPGRPDTGYYDLLASEARLASFI
                     AIAKGDVPEEHWFHLGRLLVDADGRPTLVSWSASMFEYLMPMLLLRNYPGTLLDAACR
                     GAVAHQVAFASRHGIPWGISESGYPLMDRAGHYQYKAFGVPGIGLKRGLGEELVVAPY
                     ATFLAAQVDLEESLRNLKKLARAGLEGPFGFYEAIDCTRDPGDGRGDVAGRPRKAGDT
                     GVIVRSFLAHHQGMTLLALANVLRKGIFQRRFHCDPRMQATELLLQERLPRRVSIGPM
                     RPAEESRATPVLTGGAVRRYRSPHTPAPHTHFLSNGTYTAFVTNAGGGASLCRGRAVT
                     RWREDMTRDHGSQFLYLRDARSGRAWSAAWMPLGGEPDDYLVTFELEKATLQRTEDDI
                     ETRLDITVSPEEDMEVRRLSITNRGSRPRDIEVTSYVEFALAEPAADLAHPAFHRLFL
                     ESSWVPDSHAVLVARRPREAGEAAPCGVHVVSVDGRLAEPVEY"
BASE COUNT          785 a         1636 c         1830 g          721 t
ORIGIN      
        1 gtactcgacg ggttcggcca ggcgcccgtc cacgctcacg acgtgcacgc cgcacggcgc
       61 cgcctccccc gcctcgcgcg ggcggcgcgc caccagcacc gcgtgcgagt cgggcaccca
      121 ggatgattcg aggaagaggc gatggaacgc cgggtgggcc aggtcggcgg ccggctccgc
      181 cagcgcgaac tccacgtagc tcgtcacctc gatgtcgcgc gggcggctgc cgcggttggt
      241 gatggagagg cgccgcacct ccatgtcttc ctcgggcgat acggtgatgt cgaggcgcgt
      301 ctcgatgtcg tcttccgtgc gctggagcgt ggccttctct agctcgaacg tgaccaggta
      361 gtcgtccggc tcaccgccga gcggcatcca ggcggccgac caggcgcggc cgctccgggc
      421 atcgcgcagg tagaggaact ggctgccgtg gtcgcgcgtc atgtcctcgc gccaccgggt
      481 caccgcgcgg ccgcggcaga gcgaggcgcc gccgccggcg ttggtcacga aggccgtgta
      541 cgtaccgttg gagaggaagt gcgtgtgcgg cgccggcgtg tgcggcgagc ggtagcgccg
      601 cacggcgccg ccggtgagca ccggagtggc gcgcgattcc tcggccggcc tcatcggccc
      661 gatcgacacg cggcgcggca ggcgctcctg gagcagcagc tcggtggcct gcatccgggg
      721 atcgcaatgg aagcgccgct ggaagatccc cttccggagg acgttggcca gggcgagcag
      781 tgtcatcccc tggtggtgcg ccaggaacga ccggacgatc accccggtgt cgccggcctt
      841 ccttgggcgc ccggccacgt cgccgcggcc gtcgcccgga tcgcgcgtgc agtcgatggc
      901 ctcgtagaag ccgaagggcc cttcgaggcc ggctcgggcc agcttcttga gattccggag
      961 cgactcctcg agatcgacct gcgcggccag gaaggtggcg tacggcgcca cgaccagctc
     1021 ctcgccgagg ccgcgcttca ggccgatccc cggcacgccg aaggccttgt actggtagtg
     1081 ccccgcacga tccatgaggg gatagcccga ttccgatatc ccccacggaa tgccatgacg
     1141 tgacgcgaac gccacctggt gggccaccgc accgcgacag gcggcgtcga ggagcgtgcc
     1201 cgggtaattg cgcaggagga gcatcggcat caggtactcg aacatcgacg cgctccacga
     1261 cacgagcgtc ggtctcccgt cggcatccac caggaggcgt ccgaggtgaa accagtgctc
     1321 ctcggggaca tcccccttgg cgatggcgat gaagctggcc agccgcgctt ccgaggccag
     1381 gaggtcgtag tagccggtgt cgggacgccc cggcccttcg cggtcggcca gtcgataccc
     1441 gatcgtgaag agcttccgct cacggtcgaa aaggaaacgg aagtccatga gctgcatgcg
     1501 tgcctcgcat cgttcggcga ggtcgaggag acgccggcgc cgggcaacac cggtggaacc
     1561 gtcgccgggt gcatcccggg ttgaatcggc cgccacctcg cgcagcccca cggccagcgc
     1621 caccagcgct ccggccaggt tgccggagtc gacggtcgac acgtagcgag gatgcagcgg
     1681 tgccagcgtg gccgtgctgt accagttgag gaggtgcccc tcgtgctgct cgagcccctc
     1741 gatggtgtcc atcgcccctt cgaggcgctc gacgagcccc gcctcgtcga ggaaaccgag
     1801 atcatgcgcc gccagggtgg agaggagtcc gaggccgatg ttggtgggcg aggtgcgccg
     1861 ggccagcatc tcggtgggga tctcctgcag gttgtcgggc ggcagccagt ggttgtccgc
     1921 cgtcatgtac tgcttgaagt aatcccacgt gcgggcggcg atccgctcca gccaggcgcg
     1981 ctcctcgggc gaggggtcga actccttgat ccgcgtcggc cggctccagc gccaggcgag
     2041 gagtggcgcc agcgtccaca acaggaggaa cggcgcggcc accggcaggg catgcggccg
     2101 gagcgggatg atgatcgaag tgaggaagag cgccatgatc gggctcattc ccatctcggc
     2161 caggtagagc gtgaggccgc gtccctcgag caggcgcgag acgaatgcgg ccgtttccca
     2221 ctcgaggagg cgctggcgcg tgatgaggag ccgggccagc gtgaggatga cggcgtgcgt
     2281 catccggtag gcgtgatagg ccaggaaggt gatggccatc agcgtctgcg cgagggcggt
     2341 gctcaattcg tggccgatct cgcgggtgaa gtgacgccag ctctgccccg gccccggtcc
     2401 gtgcagcgcc tgcgcgatcg gccgatacac ggggaacgcc atcgccagga ggacgccgag
     2461 agtccagctg agcggcgaac cggggagaac cgtccacgcg aacgccagga gcacgaggag
     2521 cgacggcgcg acgaggctcc gccggaggtt gtcgaagatc ttccaccggc tgatgagcgg
     2581 gaggcggttc tcggccaggc cgccgagttt gggcacccac ggcaggagcc agaggaggat
     2641 ctgccagtcg ccgcgcaccc agcgatgctg tcgccgcgtg tggacgagga cgctcgacgg
     2701 gtagtcgtcc acgacttcga gatcggaaac cagccccgtg cgggcgtgaa gtccctcgaa
     2761 gagatcgtgg gaaaggatga ggttcggcgg cacgcgcccc tcgagcgcgc tcatcagggc
     2821 atccacgtcg tagagcccct tgccggtgaa gatcccctcg cagaagaggt cctggtacac
     2881 gtcggagacc gccgtggtgt agggatcgac accggtgtgg ccggcgtaca cccgtgaaaa
     2941 aagcgaaccg gccacgctcg acatcgtcac gctcacgcgc ggctgcagga ttccgtagcc
     3001 cgaggccacg cggccgctct tcgggtcgaa gacggggcga tggagcgggt gcaaggcgat
     3061 cccgatgagc tggcgcgcca cgccgcgcgg aagtcgcgtg tcgctgtcga gcgtgatgca
     3121 gtaccgcact ttcgagagca cggtgggatc gccgacgtgc acctcgaagc cggtcgtcga
     3181 atcgccccgg atgtagcggt tgaactcctc gatcttgccg cgcttccgct cccatcccat
     3241 ccacaccttc tcgatggggt tccagcgccg gccgcggtgc agaaggtgga actggtcggt
     3301 acgtgccggg ccgtgccgcg cgttcaactc ccggatgccg gcccgggctg cctccaggat
     3361 gacggggtcg tcgggcatct ccccgctcgg cgcatcggcg aaatccgtga ccagggcaaa
     3421 gtggatgcgc gggtcgtcgt tggcgagcgc ctgcacctcg agattgtcga ggagatgacg
     3481 taccccttcc accgaggtga gcagggtcgg cacgatcacc atcgtgcgcc cctcttccgg
     3541 cacgccggac tcgaggtcga gccgcggcag ccggtgcgga cgcgccacca gtggcacgag
     3601 ccactgcacg atgtggatgg ccaggtcgct tgccgggagg atcaggaggc cggtgatcag
     3661 cgggtcgaag cctccgccgg ggatgaggcg gagcgccagc cagacgagga acccggtcag
     3721 gagaaggatc gagccgatgt agagcggcgc cgcagccgag aagacggccc gacggagcga
     3781 ccggatgccg cgagcggggt gttcgctggc cgcttcgagt tgggggcgcc cgccccccag
     3841 gatgtagtga cccacatgcc cgacccggac atcgtgcggc gccgactggg cgccacgccg
     3901 ggccagctcc accgccttga gcgccacgcc gatttccgat tcgccggtcg gcccggccag
     3961 cgcctcgatg gcctcgcggt agcggttgcg actggcgaag tccatgcggg cgtaaacgcc
     4021 ggaaggatcg cgtcgcaggg cctgctcgac caggctcacc cgctcgaaat tgcggttcca
     4081 gtcgagcgtg gaacagagcc tgagcccggt gatcgcgttt gccatcgagg tctgcgccgc
     4141 ggcctgggcc acatgctcgg cccggatggc ctcctcgggc gagaggccgc gcgcggccag
     4201 gcgctccgac aactgcaccc gcaacggcga aacgcgcggc ccgaattctc gaaagcgctc
     4261 gaggagctgg gccacggagg cggcgggcag ggggtcgggg aggggcggca tcggctcgtc
     4321 ctctcccgcg gcttcgaaac gatggatgaa gcggtcggcg gtgaggcgcg catcgcggct
     4381 caccatcagc tgctcggcca gtcgtcggat gttctcgatc agctcggcct tgagaagcgt
     4441 gggccacgcc cacaactcgc cgatcgtcag cggcacgagc gtctggaacg cggtgatgta
     4501 gcggatgagc cgcgcgaggt cgatcgaggc gtcgctgtga cgcaggagct cgatggccag
     4561 cgcctggatg cgcgggcggc cggcatggtc gccgccggtg agcaccggca gccggtcgat
     4621 gaagcgcttc ggcatgttgc ggcggatgtc cgccagttcg gcctcgacca ggtggaaatt
     4681 gtcgagcagc cactcggcgg ccgggagcgg cgattcggcg ccggcggcgt ccgcaatcag
     4741 cttgcggtag gcgtcgcgca ggatgcgggc gccggcgttg agccgctcgt gaaagacgtg
     4801 gccggcctca ccggtcgact cccccacggc aaaccggccg gccagcaggc gggcatgcgc
     4861 ctcgagcccc tcgggcgaca ggatgtcgcg gtgccgctgc ggcgcggtgg ccgtttcgat
     4921 gatgtcggtc gtcgtgggag tgatcgtggc tgtcgacatg ggtggcgcgg tc
//
LOCUS       DASVOD010000666         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_617170, whole genome shotgun sequence.
ACCESSION   DASVOD010000666 DASVOD010000000
VERSION     DASVOD010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617170"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF720_14285"
     CDS             complement(<1..309)
                     /locus_tag="VF720_14285"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX7880579.1"
                     /translation="MPCALPLAAYLAAQLILSELMIKPPSGGSEWIEIFNAGDAPIAT
                     TGLSIEDARGRPAKFPEQPAELAPGTYLVLASNVERVLEQFTDLDAARVVKPAGTWPA
                     L"
     gene            complement(464..2749)
                     /locus_tag="VF720_14290"
     CDS             complement(464..2749)
                     /locus_tag="VF720_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880580.1"
                     /translation="MPAISDFKGFSFQPPGGHLWPWFLVGILLVLLALWVYRRNPAPM
                     SRPLKGVLVAIRAAAFLILLLLICRPVISLGAADRGRRLVALLVDTSESLTLPAGGDA
                     GGATRADQVRTVLGEIRSGLADRHGLRAWGFDRQARPLAEDLKLTPPPEALAPVGDVT
                     AMGPAVEAAVAEIGRARAGAVVVVSDGVANQGIDPVAVARRLSLPVYTVGVGSDSIRL
                     DAAVARIQVNRTAFLGDDVPLSVTVHNTGVGGTSVELDVVDVTRPGREEVVAKQALVW
                     ANEGAEQQVRMKFRPSSVGQHFYEVRVPARADEFTSVNNRRMFALDVREEKNRVLVIA
                     GTTDWDLTFFGRALAADSSLSVTTMVRMGGQWRSIEKASQSVSFAPDAASLGKYVLVV
                     LDGLDARDLSAASWEALAAWTRRGGGLLVFGGAQGSGIRRLAGTPVAGLLPVDPASAQ
                     SNGSSSIGVTLTAAGGRHPATEVYEGESDNTAAWTDLPPLSSASAEPAVKGGAEVLVS
                     GSDEFPVLVAGRAGAGKVLVAPAAGFWRWAFRPEGREDRRDFHERLWVNAARWLTSPD
                     LASRLHVEPGRPVFERGDAVDITARLADGDYQPLDGAEINVTITRDDSLTTPRAPGAP
                     EEPAVQPRSLSLAGSGGGFYAGETGSLKPGRYRFRAQARKGDKSLGEATGSFAVETMG
                     LEFRRPAADPGFLARLAAESGGTYYAAADARRLPEELVVSGKSSQEVVTLDIWDSPWF
                     FVAFVGLLSSEWFLRRRRGMV"
     gene            complement(2872..3798)
                     /locus_tag="VF720_14295"
     CDS             complement(2872..3798)
                     /locus_tag="VF720_14295"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX7880581.1"
                     /translation="MFVEEYLVDLRREGYTPAAWRRYVDRSFRLARESAFERPAMLRS
                     IALAGVLGFLVLLGVSLGAAFVAGARLSVEIFSQCGLMLLAGVAIMAGHVRLLVRPDG
                     TAVERINPANLLTLSRLVAIPAMLALVRHGFLPAALLTFLLGGFTDVLDGWLARRRGD
                     ATDFGRIFDPIVDIAFHLAIFLALHAVGLIPDWVLVLVLLRYGLLAFGAVGISVFRGP
                     VEIRPTVLGKTTGVVMTMLVAMLVAGRLTLPSAVSGRIETLIVLAIGFVEAITIPQVL
                     LIGWYNFRKAGRGTPPVIALVSSDEESARRLR"
     gene            complement(3849..4559)
                     /locus_tag="VF720_14300"
     CDS             complement(3849..4559)
                     /locus_tag="VF720_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009095964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7880582.1"
                     /translation="MTGGLKVAVVIPALNEAASIGRVLADLPRPPVGQVVVVDNGSTD
                     STAEVARKGGAMVLSETRRGYGQACLTGIDWLKEAGRRPDILVFVDGDYSDHPAELVR
                     VIEPITSGRADMVIGSRVLGEAAEGALLPQARFGNWLATRLIRWIWGVPYTDLGPFRA
                     IRFDALERLEMADTNFGWTVEMQVKAARAGLAVSEVPVSYRKRIGKSKITGTLKGTMA
                     AGLKILWTIAREAVTPRA"
     gene            complement(4556..>4872)
                     /locus_tag="VF720_14305"
     CDS             complement(4556..>4872)
                     /locus_tag="VF720_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019013241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEX7880583.1"
                     /translation="RDDIGKFLHIYIEGRILPEDPFVVLDQAGVGDLMRLAAERGRKA
                     RPDLKLGICGEHGGEPSAVAFCHGLGFQYVSCSPYRVPIARLAAAQAALADVSGIGNR
                     TA"
BASE COUNT          805 a         1676 c         1736 g          655 t
ORIGIN      
        1 cagcgccggc caggtgccgg ccggtttcac gacgcgcgcc gcgtcgaggt cggtgaactg
       61 ctcgagcacc cgttcgacat tcgacgcgag gaccaggtaa gtgcccggtg ccagctcggc
      121 cggctgttcg ggaaatttgg ccggccggcc gcgggcatcc tcgatgctga gtcccgttgt
      181 ggcgatgggg gcatcgccgg cgttgaaaat ctcgatccac tcggagccgc cgctgggcgg
      241 cttgatcatc agctcactga ggatgagttg cgccgccagg tacgcggcaa gcggaagtgc
      301 gcagggcatg tccgtctccc gggtcggccg gcactgtgcc ggaccgccca acggacggga
      361 ccgtcagggg agcgtggcgg aacgaatgga cgggtgaggc gcgacgggtg cgagtcgtat
      421 cgggtttcgg aacttgaggg tgccgggcgg tggatcgccg gccttacacc atcccccgcc
      481 gtcgtctgag gaaccactcg gacgagagca atcccacgaa ggccacgaag aaccaggggc
      541 tgtcccagat gtcgagcgtg accacttcct ggctcgactt gccggagacg accagttctt
      601 ccgggagccg cctggcatcg gcggccgcgt agtacgtgcc gcccgactcg gcggccaggc
      661 gggccaggaa gcccgggtcg gccgccgggc ggcggaactc gaggcccatc gtctcgacgg
      721 caaagctgcc ggtggcttcg ccgagtgact tgtccccttt ccgggcctgg gcgcggaagc
      781 ggtagcgccc cggcttcagg ctgccggttt cccccgcgta gaatccaccg ccggaaccgg
      841 ccagcgagag cgagcgcggt tgcacggcgg gttcttcggg cgcaccgggc gcgcgcggcg
      901 tcgtgagcga gtcgtcgcgc gtgatcgtca cgttgatctc ggcgccgtcg agcggctggt
      961 aatcgccgtc agccaggcgt gccgtgatgt cgacggcatc gccgcgctcg aagaccggac
     1021 ggccgggttc cacgtggagg cggctggcca ggtcgggcga ggtgagccag cgggccgcgt
     1081 tgacccagag tcgttcgtgg aagtcgcggc gatcctcgcg cccctccggc cggaaggccc
     1141 agcgccagaa accggcggcg ggcgccacga gcacttttcc cgcaccggcc cgccccgcca
     1201 ccagcaccgg gaattcatcg ctgccggaaa cgaggacctc ggcgcccccc ttcacggccg
     1261 gttcggccga ggcgctggaa agaggcggga gatcggtcca cgccgcggtg ttgtcggact
     1321 cgccttcgta cacctcggtg gcggggtgcc gcccaccggc ggcggtgagc gtgacgccga
     1381 tgctcgagga accgttgctc tgcgccgagg cgggatcgac cggcaggagt cctgcaaccg
     1441 gagtcccggc caggcgccgg atgccggagc cctgggcacc gccgaagacc aggaggcccc
     1501 ccccgcgccg ggtccaggcg gccagcgcct cccacgacgc ggcggaaaga tcgcgcgcgt
     1561 cgaggccatc gagcacgacc agcacgtact tgccgagcga ggccgcgtcg ggcgcgaacg
     1621 acaccgactg gctggccttc tcgatcgagc gccactgccc ccccatgcgt accatggtcg
     1681 tgacggagag gctcgagtcg gccgccagcg cccggccgaa gaaggtgagg tcccagtcgg
     1741 tcgtgccggc gatcacgagg acgcggttct tctcttcgcg cacgtcgagc gcaaacatgc
     1801 gccggttgtt cacgctggtg aactcgtcgg cgcgcgccgg cacgcgcacc tcgtagaagt
     1861 gctgtcccac gctcgacggg cggaacttca tccgcacctg ctgctccgct ccctcgttgg
     1921 cccagacgag ggcctgcttc gccaccactt cctcgcggcc cgggcgcgtc acgtccacca
     1981 cgtcgagttc gaccgaggtg ccgccgacgc ccgtgttgtg gacggtgacg gagagcggca
     2041 cgtcatcacc caggaaggcg gtacggttca cctggatgcg cgccacagcc gcatcgaggc
     2101 ggatcgaatc gctccccacg cccaccgtgt agacgggcag cgagaggcgg cgggccacgg
     2161 ccacgggatc gatcccctgg ttggccacgc cgtcggaaac caccaccacg gcgccggccc
     2221 tggcgcggcc gatctcggcc acggccgcct cgaccgccgg gcccatggcg gtcacgtcgc
     2281 cgacgggtgc cagcgcctcg ggcggtggcg tgagcttcag gtcttccgcc agcgggcgtg
     2341 cctgccggtc gaatccccag gcacggaggc cgtgcctgtc ggccaggccc gaacggattt
     2401 cccccagcac ggtgcgcacc tggtccgccc gggtggcgcc cccggcatct ccgccggcgg
     2461 gaagagtgag actttcggac gtatcgacca ggagcgccac gaggcgacgc ccgcgatcgg
     2521 ccgcaccgag ggaaatgacg ggccggcaga tgagaaggag gagaatcagg aaggccgcgg
     2581 cccggatggc caccagcacc cccttgagcg ggcgcgacat cggcgccggg ttgcgccggt
     2641 agacccagag ggccaggagg acgaggagga tgccgacgag gaaccagggc cagagatggc
     2701 cgccgggcgg ctggaacgag aaacccttga agtcgctgat ggccggcact ccctgcaaag
     2761 tcccaaacgg tcgttccggt tcccggaaag accgacggcg attcgatttg tggaacggga
     2821 tcgaacatgg acgggcgggt gcgaaacgct aacaggggtt cgttggaagt gtcaacgaag
     2881 gcgtcgtgcc gattcctcgt cactggatac aagcgcgatg acgggcggcg tcccgcgccc
     2941 cgccttccgg aagttgtacc agccgatcag gaggacctgc gggatggtga tggcctccac
     3001 gaagccgatg gcaagtacga tcagcgtttc gatgcgcccg gacacggcgg atggcagcgt
     3061 caggcgtccc gccacgagca tcgccaccag catcgtcatc accacgccgg tcgtcttccc
     3121 gagcacggtg ggacggatct cgaccggccc acggaaaact gagatgccga cggcgccgaa
     3181 ggccaggagt ccgtaacgaa ggagcaccag cacgagtacc cagtcgggga tgaggcccac
     3241 cgcgtgcagc gccaggaaaa tcgccaggtg gaaggcgatg tcgacgatcg ggtcgaagat
     3301 gcggccgaag tcggtggcat cgccccggcg gcgggccagc cagccgtcga ggacgtcggt
     3361 gaagccgccg agcagaaacg tgagcagcgc ggccggaagg aagccgtgcc gcaccagtgc
     3421 cagcatcgcg gggattgcca cgaggcgcga cagcgtgagg agattggccg gattgatccg
     3481 ctccaccgcg gtgccgtcgg ggcgcacgag gagccggacg tggccggcca tgatggccac
     3541 gccggccagc agcatgaggc cgcactggct gaaaatctcc acgctgagcc gcgcccccgc
     3601 cacgaacgcc gcgcccagcg agacgccgag cagcacgagg aagccgagca cgccggccag
     3661 cgcgatggag cggagcatgg ccggacgttc gaaggccgat tcgcgggcca gtcggaacga
     3721 ccggtccacg tagcgtcgcc aggcggcggg cgtgtatccc tcccgccgga ggtcgaccag
     3781 gtactcctcg acgaacatgg ccccccgagt gtatcagccg gagcggggtg tcggtcggtg
     3841 cgcgaggctc aggcccgggg cgtcacggcc tcccgggcga tcgtccatag tatcttcaac
     3901 ccggcggcca tcgtcccctt cagggtcccc gtgatcttgg acttgccgat ccgcttccgg
     3961 tacgacacgg ggacctcgga aaccgcaagg ccggcgcggg ccgccttcac ctgcatctcc
     4021 accgtccacc cgaagttggt gtccgccatc tccagacgtt ccagggcgtc gaaacggatg
     4081 gcccggaaag ggccaaggtc ggtgtacgga acgccccaga tccaccggat gagccgcgtg
     4141 gccagccagt tgccgaaacg cgcctgcggc agcagggctc cctcggcggc ctcgccgaga
     4201 acgcgggagc cgatgaccat gtcggcccgg ccggacgtga tcggctcgat cacgcgcacg
     4261 agctccgccg ggtggtcgga gtaatcgcca tcgacgaaga ccaggatgtc ggggcggcgc
     4321 ccggcctcct tcaaccaatc gatgccggtg aggcaggcct ggccatagcc gcggcgcgtc
     4381 tccgacagga ccatggcccc tcccttgcgg gccacctcgg cggtgctgtc ggtggagccg
     4441 ttgtcgacca ccaccacctg cccgaccgga ggccgcggga gatcggccag cacgcggccg
     4501 atggaggcgg cctcgttcag cgcgggaatg acgacggcca ccttgaggcc accggtcacg
     4561 ccgtgcggtt tccgatcccc gagacatcgg ccagcgcggc ctgggccgcc gccagccttg
     4621 cgatcggcac ccggtacggg ctgcacgaca cgtactggaa tccgaggccg tggcagaagg
     4681 ccaccgcgct cggctccccg ccgtgctcgc cgcagatgcc gagcttcaag tcggggcggg
     4741 ccttgcgtcc ccgttccgcg gcgagacgca tcaggtcccc cacgccggcc tggtcgagaa
     4801 ccacgaacgg atcttcgggg aggattcgcc cctcgatgta gatatgcagg aacttgccga
     4861 tgtcgtcgcg cg
//
LOCUS       DASVOD010000667         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_773550, whole genome shotgun sequence.
ACCESSION   DASVOD010000667 DASVOD010000000
VERSION     DASVOD010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773550"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1740
                     /locus_tag="VF720_14310"
     CDS             <1..1740
                     /locus_tag="VF720_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880584.1"
                     /translation="PRNTESALDGLHRHRPVVAGLVLVVAIAMDLVGPVPGRRELLFG
                     VALLDLAGELVSRMLTGRFEPARLLLTGTLAADVVIATLVVSLLGGATSPFVLLFPLL
                     AFTGGMLAGMGAGVVLGVFSAIGYECAAVLSGGVALGPDGTPEVVAGARVAFQMAFYP
                     VFFTLVGILSGLLGRRLAENERALEVAADEMEKLRLDTESIIQNLSSALLTIDRAGRI
                     VHLNRVAEMLLGVTAAHVRGHKLGEALKPGHRELVEKIETTLQEDLPLLRAEVRFQRG
                     GEEAGTGAGSRGAAENRGAEPETESATGSPGWAPLGVSTSLIKDRRGNRSGVVALFQD
                     LTEVRRLEEAGRRQDRLSVIGGMAASIAHEVRNCVNPISGSVEILQSELALEGENAKL
                     LELIGREAGKMERLVSELLNFSRGNPMTVEPTTVESILDATLDKVRAHPAWKPTVELV
                     RNWSGEPTAIRADVHQLEQVFFNLALNALEAMGPVGTLTVTVEPNEAGSTVEFADTGA
                     GMTRETLERIFEPFYTTKGSGTGLGLAIAHRIVERHEGRMEVSSRPRMGTRVRVTLPN
                     QPAHTVPTRKAAA"
     gene            1784..3202
                     /locus_tag="VF720_14315"
     CDS             1784..3202
                     /locus_tag="VF720_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7880585.1"
                     /translation="MGSEKILVVDDEPGMCQFLAIMLRKEGYQVTTASNGPEALEHVR
                     GEEFHCVLTDVKMPGMDGLELLDAIHELDATLPVVVMTAYASEKASVDAMNRGAFQYL
                     EKHAKNDEIKLVIKNALNMKVVQSENVFLKRQLKKTQSNKEIIGASEEMHRVFKLIDK
                     VAETDSTILISGESGTGKELIAKEMHYRSKRANGPFVSINCGALPKDLLESNLFGHMK
                     GSFTGAVKDQAGLMQVAEGGSFFLDEVGEMSPATQVKLLRALQEREIIPVGGTKPIKI
                     DTRLIAATNADLEKDVRDGKFRSDLFYRLNVIPLKLPPLRMRRDDIPILVDHFLRKFA
                     SNGGVLKTMSKDCMTILMRYDWPGNVRELENVIERAVILSDSQQLITEDLPERVLRGD
                     KRKGSLIIDCPEMTLEELEREYILKVLNYTGWQKKRASTILGINASTLYRKLQAYKLE
                     GHLQGRDISIDEMPDEAIEEAA"
BASE COUNT          742 a         1361 c         1449 g          720 t
ORIGIN      
        1 cccaggaata ctgaaagcgc actcgacgga ctgcaccggc accggccggt cgtggccgga
       61 ctggtcctgg tcgtggcgat cgccatggac ctggtcggcc cggttcccgg ccgccgggag
      121 ctgctgttcg gtgtggcgct gctggacctg gcgggggagc tggtcagccg catgctgacc
      181 gggcgattcg agccggcccg gttgctgctg accggcacgc tcgcggccga tgtggtgatc
      241 gccacgctgg tggtgtcgct gctcggcggg gccaccagtc cgttcgtgct gctgttcccg
      301 ctgctggcgt tcacgggcgg gatgctggcg gggatggggg ccggcgtcgt gctcggggtc
      361 ttctcggcga tcggctacga atgcgcggcg gtgctgtccg gcggtgtggc gctggggccc
      421 gacggcacac ccgaggtggt ggccggcgcc cgcgtggcat tccagatggc gttctacccc
      481 gtgttcttca cgttggtcgg catcctgtcg ggcctgctcg gccgccgcct cgcggagaac
      541 gagcgcgccc tcgaggtggc cgccgacgag atggagaagc tccggctcga taccgagtcg
      601 atcatccaga acctctcttc ggccctcctc accatcgacc gggccggccg catcgtgcac
      661 ctgaaccggg tggccgagat gctcctcggc gttaccgcgg cccatgtgcg tggtcacaag
      721 ctcggggagg ccctcaagcc gggacaccgc gagctggtgg agaagatcga aacgacgctc
      781 caggaagatc tccccctcct ccgcgcggag gtgcgctttc agcgcggcgg ggaagaggcg
      841 ggcacggggg cggggagccg tggtgctgcc gagaaccgtg gggccgagcc tgagacggag
      901 tcggccaccg gctcgcccgg ctgggcgccg ctcggggtat ccacgtcgct catcaaggat
      961 cgtcgcggca atcgcagcgg ggtcgttgcc cttttccagg acctgactga agtccggcgg
     1021 ctcgaagaag ccggccggcg acaggaccgg ctgtcggtga tcggcggcat ggccgcctcc
     1081 atcgcgcacg aggtccgcaa ctgcgtgaac ccgatctcgg gctcggtcga gatcctccag
     1141 tcggagctcg ccctcgaggg ggaaaacgcc aagctgctgg aactgatcgg ccgcgaggcc
     1201 ggcaagatgg aacgtctcgt ctccgaactt ctgaacttca gccgtgggaa cccgatgacc
     1261 gtcgagccga cgaccgtgga gtcgatcctg gatgccacgc tcgacaaggt ccgggcgcat
     1321 cccgcctgga aacccaccgt ggagctggtt cgcaactggt cgggtgagcc gaccgcgatt
     1381 cgcgcggacg tccaccagct cgagcaggtc ttcttcaacc tggccctgaa tgccctcgag
     1441 gcgatgggcc cggtggggac gctcaccgtg accgtggagc cgaacgaggc cgggagcacc
     1501 gtcgagttcg ccgatacggg cgccgggatg acccgcgaaa cgctcgaacg cattttcgaa
     1561 ccgttctaca cgaccaaggg aagcggaacc gggctcggcc tggccatcgc gcaccgcatc
     1621 gtggagcgcc acgaggggcg gatggaagtg tcgagccgcc cccggatggg cacacgggtc
     1681 cgcgtgaccc ttccgaacca gcccgcgcac accgttccga ccagaaaggc cgccgcatga
     1741 accgcaacac gcaaccgatc cgagggggtg aatccaggtg atcatgggat ccgagaagat
     1801 tctcgtcgtt gatgacgaac cgggcatgtg ccagttcctg gcgatcatgc ttcgcaagga
     1861 aggctaccag gtgaccaccg ccagcaatgg tcccgaggcc ctggaacacg tgcgcgggga
     1921 agagtttcac tgcgtgctca ctgacgtgaa gatgccgggc atggacgggc tggagctgct
     1981 cgatgccatc catgagctcg atgccaccct tccggtggtg gtcatgaccg cctatgcgtc
     2041 ggaaaaggcg tcggtcgacg ccatgaatcg cggggcgttc cagtatctcg agaagcatgc
     2101 caagaacgac gagatcaagt tggtcatcaa gaatgccctc aacatgaagg tggttcagtc
     2161 ggagaacgtc ttcctgaagc gccagctcaa gaagacccag tccaacaagg agatcatcgg
     2221 ggcttccgag gagatgcacc gggtcttcaa gctgatcgac aaggtggccg agaccgattc
     2281 caccatcctg atttccggcg agagcggcac cggtaaggaa ctgatcgcca aggagatgca
     2341 ctaccggtcg aagcgggcca acgggccttt cgtgtcgatc aactgcggcg cgctgcccaa
     2401 ggacctgctc gagtcgaacc tcttcggcca catgaaaggg tcgttcaccg gcgccgtgaa
     2461 ggaccaggcc ggcctcatgc aggtggcgga gggaggctcg ttcttcctcg acgaagtcgg
     2521 ggagatgagc cctgccaccc aggtgaagct gctcagggcg cttcaggagc gcgagatcat
     2581 cccggtcggc ggcaccaaac cgatcaagat cgatacgcgt ctgatcgccg ccaccaacgc
     2641 ggacctggag aaggatgtcc gggacggcaa gttccggagc gacctcttct accgactcaa
     2701 cgtgatcccc ctgaaattgc cgccgctgag gatgcgccgc gatgacatcc ccatcctggt
     2761 cgaccacttc ctccggaagt tcgccagcaa cggcggtgtg ctcaagacga tgagcaagga
     2821 ctgcatgacg atcctcatgc ggtacgactg gccgggcaat gtccgggagc tggagaatgt
     2881 gatcgagcgt gcggtcatcc tgtcggacag ccagcaactc atcacggagg acctccccga
     2941 gcgcgtgttg cgcggcgaca agcgcaaggg cagcctgatc atcgactgcc ccgagatgac
     3001 gctggaggaa ctcgagcggg agtacatcct caaggtgctc aactacacgg gctggcagaa
     3061 aaagcgcgcc agcacgatcc ttgggatcaa cgcctcgact ctctaccgga agcttcaggc
     3121 ctacaagctc gagggtcacc tccagggtcg cgacatcagc atcgacgaaa tgcccgatga
     3181 ggccatcgaa gaggctgcgt aaagggcagc cgcgctcgcc agcgcccgca atgtccgacg
     3241 aggcccggtg ttcatgcacc gggcctcact gtttttcgga agggccgggc ggcttgcagg
     3301 cgtccgattg acctcggccg tgatggccgt tcggtcgacg gcggggtgaa ctcaaccgtg
     3361 gggcccgact tgttgacgag ccgggcggag catcacacgg acttgcggaa ctttcaccgg
     3421 tgcgagttcg cgggactggc ctccccatcg tctgttcggc ttcgtacgca ttgtgggccc
     3481 tgcaccgcag ggtgaggttg gcgctcgtgg aggctccacc gcgcgaccac ggcaggatgt
     3541 ggtggaactc gagttgctct gtctcctcgc agtgctgacc ggcagtgccc tcgaacgtgc
     3601 agcgcccttc gtcgcggtgc cacacggcgc gacgaaccga tggcgatatg tatcgggagg
     3661 gttcccgccg ttcgacggga cgtgattcct gacttgccgg cgccgtgggg cggattttcg
     3721 ccgcagtgcg cttcccccca ggtcgcgttc cccccggccg gaccctcgct tccggtgccc
     3781 cgcggtccga aggccaggct cctgctcctt ggactgccgg gtcgttggcc agcctcccga
     3841 acttccggcg ttcgagcacc actatcaggg cggcaagtgc cctgtcgagg atcggcgcca
     3901 ggtcaccggt gggattgatg tggctcatca ggtgctgggc ccgctccagc cggacccggg
     3961 tggcttcgtt gatcgtgaca tgcaaacggt agcggcccgg ctcgtcgtcc atgccctgct
     4021 ctgaaggagg cttcggtggg atgccaggta catccgttcc ctcaacggat tcaggatcat
     4081 ggcgcgccgg cacaggtgcc ccgtcgaaga gggcgagcat tgggggtcca gcaccagcca
     4141 gattcctgac gcgctctgcg tgagtacctg gcggatggtg cctcccggtg tgctctccgg
     4201 atgccgggtc tccgacagcg gtaaggatag tcgccgcccc gggcaagtcg acgattgcca
     4261 cagatgtcga tt
//
LOCUS       DASVOD010000668         2185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_200769, whole genome shotgun sequence.
ACCESSION   DASVOD010000668 DASVOD010000000
VERSION     DASVOD010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200769"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VF720_14320"
     CDS             complement(<1..665)
                     /locus_tag="VF720_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880586.1"
                     /translation="MSDAMKPAGPRDGAPNDLDTWLLAGVEAHYLGRSSFLKDFDALA
                     RGTADLAGAHGGLVFLAGRSDGGLPVEGAAGLDTVPERAALLTAARTLVRSAMSAGTP
                     RQAEAATSDEGHVLPPIFVEPIVARGQSLGALVVFGAWHDDVSARMTRAARLAAHLVT
                     ERLLQQETEDLKRRLGAAEREKARLGPQAELGAATRDVAGASLRALADVLAFGRRVAR
                     ELPP"
     gene            769..1086
                     /locus_tag="VF720_14325"
     CDS             769..1086
                     /locus_tag="VF720_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX7880587.1"
                     /translation="MPETDKPQQPAPRPQGPNIQIEIPEKEAEGIYANLAFIQHSPSE
                     VVIDFARVLPSVPKSKVYARIVMTPYNAKALLMALEENLKRYENQFGPIKVGPNEGRS
                     IGF"
     gene            complement(1083..2057)
                     /locus_tag="VF720_14330"
     CDS             complement(1083..2057)
                     /locus_tag="VF720_14330"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7880588.1"
                     /translation="MTSSARVSFRHRLEAFLLRALSGFFCLLPHRLAVRTGGGLGWLV
                     SRVAPIRSKVADENLRRAFPLMGEAERRDILDRFYRHLGRMLAEFLRSPALDAARIVE
                     WTNPEGEEHLKAAAAEGRGVVMISGHLGNWEWLVASAAARGYALDVIVAPQKNIAVQD
                     FITRCRAGVGVGVLYSKRGRGELLEAGRRLRKGHLLGTLVDQDAGRRGWFVDFLGSPA
                     SAAVGTFRMALAARAPVVCVFTWREGFRHHVVAEPALVRDPTLDDEAQAREWAAEFHR
                     RLEARIRAHPEQWFWVHRRWRSRPANEAAPVVPGPGRAMVAPDPGSAA"
BASE COUNT          377 a          806 c          717 g          285 t
ORIGIN      
        1 ggcgggagtt cacgcgccac gcgccgtccg aaagccagca catccgccag cgcacggagc
       61 gaggctccgg cgacatcacg cgtggccgca cccagctccg cctgcgggcc gagtcgggcc
      121 ttctcgcgct ccgccgcgcc gagccgacgc ttgaggtctt ccgtctcctg ctgcaggagc
      181 ctctcggtga cgagatgcgc cgccaatcgc gcggcgcgcg tcatccgggc cgacacatcg
      241 tcgtgccagg cgccgaagac gaccagcgca cccagcgact gcccgcgggc cacgatcggt
      301 tcgacgaaga tcggcggcag gacgtgtccc tcgtccgacg tggcggcctc cgcctggcgc
      361 ggcgtgccgg ccgacatggc cgaccgcacc agcgtgcggg ccgcggtcag gagtgccgcg
      421 cgttccggca ccgtgtcgag gcccgcggct ccttcgaccg gaaggccacc gtcgctccga
      481 ccggccagga agacgagtcc gccatgtgcg ccggccaggt cggccgtgcc gcgggccagc
      541 gcatcgaaat ccttgaggaa tgacgatcgg cccaggtaat gagcctcgac gccggcaagc
      601 agccaggtat cgagatcatt gggcgctccg tcgcgaggac cggctggttt catggcatcg
      661 gacatgcccg attcatcggc agccacgggc caactcttga gccggaactg gtttgacccc
      721 aaaggcttgg cagggtacgc tccacggccc tttgaaggag gaaccgagat gcccgagact
      781 gacaagccac agcagcctgc cccgcgcccg cagggcccta acatccagat cgagatcccc
      841 gaaaaggaag ccgaggggat ctacgccaac ctggccttca tccagcactc gccgtccgaa
      901 gtggtcatcg acttcgcgcg ggttctgccg agcgtaccca agagcaaggt gtatgcgcgc
      961 atcgtcatga ccccctacaa cgccaaggcc ctcctcatgg ccctcgagga aaacctgaag
     1021 cgctatgaga accagttcgg cccgatcaag gtgggcccga acgaagggcg atcgatcggc
     1081 ttctaggcgg cgcttccggg gtccggcgcg accatcgccc tgccggggcc cggcacgacc
     1141 ggcgccgctt cgtttgccgg ccggctccgc cagcggcggt gcacccagaa ccactgctcg
     1201 gggtgcgcgc ggatgcgcgc ctcgagccgc cggtggaatt ccgccgccca ctcacgggcc
     1261 tgggcttcgt cgtcgagcgt gggatcgcgg acgagcgccg gctccgccac cacgtggtgc
     1321 cggaatccct ccctccaggt gaagacgcag acgacaggcg cccgggcggc cagcgccatg
     1381 cggaaggtgc cgacggccgc cgatgccggc gagccgagga agtcgacgaa ccacccgcga
     1441 cgcccggcat cctggtcgac cagcgtcccg agcaggtgac ccttccgaag tcgacgtccg
     1501 gcttcgagca gttctccgcg cccccgcttg gaatacagca cgccgacacc gacacccgcc
     1561 cggcaacgcg tgatgaaatc ctgcacggcg atgttcttct gcggcgccac gatcacgtcg
     1621 agcgcgtacc cccgcgccgc ggccgatgcc accagccatt cccagttgcc gagatggccc
     1681 gagatcatca ccacgcccct cccctcggcc gccgccgcct tgagatgctc ctccccctcc
     1741 ggattggtcc actccacgat gcgcgcggca tcgagcgcgg gcgaacggag aaactcggcc
     1801 agcatgcggc cgagatgacg atagaagcgg tcgaggatgt cccggcgctc ggcctcgccc
     1861 atcaggggga acgcgcggcg caggttctca tcggccacct tgctgcggat cggggccacg
     1921 cgcgagacga gccagccgag gccgcccccc gtccgcacgg ccaggcggtg cggcaggagg
     1981 cagaagaacc ccgagagcgc gcggagcagg aacgcctcga gtcgatggcg gaacgacacg
     2041 cgcgcggatg aagtcatggc aagccatcct accctctcac gaacccccca ctccaccggc
     2101 gggccacgcc ggccatctcg agatcgagca ggtcggcgag gagtgaaggg atcggacgac
     2161 cggtggttcc cgccagctct tccac
//
LOCUS       DASVOD010000669         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_462409, whole genome shotgun sequence.
ACCESSION   DASVOD010000669 DASVOD010000000
VERSION     DASVOD010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462409"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VF720_14335"
     CDS             complement(<1..452)
                     /locus_tag="VF720_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018344990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcaP family trimeric outer membrane transporter"
                     /protein_id="HEX7880589.1"
                     /translation="MKFSSKSVFAIVATAVVLVAGNARADESSRTEIYGFVMMDAGYN
                     ASAMHPDWFDTQRPTKLESFENEFGEDGETFFSVRQTRLGVKSYLTTGMGELFTQFEF
                     EMFGTGVDAGQTTIRLRHAYGELGHFGAGQYWSPFMDIDVFPNTVEYWG"
     gene            777..1667
                     /locus_tag="VF720_14340"
     CDS             777..1667
                     /locus_tag="VF720_14340"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX7880590.1"
                     /translation="MNDDMGGLPAPEMANDFGATRPDEGPRLPGESRLSRVASRLSSA
                     SIADLDQTIVDGLCQAVELLDLDRSAVWQFTESGDDLVYTHVWDRPGSAAMRHPFSVG
                     TLFPWHRAEIREDRAIWNERVDDVPSAVDRQSLLKLETAANAVVPMRSDGTLIGALSF
                     DSCRRSVHWSPEVRDCMRVLASLFSQTIARARKDRQLGLALDEIARLRNQVVRERIGA
                     GTLSSSPQPESGPSPEPLRLSYVESAHIRHVLERSGWRIRGANGAAEMLGMKPTTLES
                     RMVKLGIHRPPRRIVRAPGY"
BASE COUNT          348 a          651 c          576 g          345 t
ORIGIN      
        1 ccccagtact cgaccgtgtt cgggaacacg tcgatatcca tgaaggggct ccagtactgg
       61 ccggcgccga agtgacccag ctcgccgtag gcgtggcgca gtcggatggt cgtctgaccg
      121 gcgtccacgc cggtaccgaa catttcaaac tcgaactgcg tgaaaagctc gcccatcccg
      181 gtggtgaggt aggacttaac gcccagacgc gtctggcgga cactgaagaa ggtttcgcca
      241 tcctcgccga attcgttctc gaacgactcg agcttcgtcg ggcgctgcgt gtcgaaccag
      301 tccgggtgca tcgcgctggc gttgtagccg gcatccatca tgacaaaccc gtagatctcg
      361 gtccgtgacg actcgtcggc tcgcgcattc ccggcgacga gcacgacggc cgtggcgaca
      421 atcgcgaata cgcttttcga gctgaacttc attgcgactt cctccctgag tccttcaccg
      481 gttcaccacc ccggacgggg ccgaccggga cggcgtcaca tcccacccga gtgcctcgcg
      541 ggaatcacgg gtccgacctg ggtatatccc cgacctgata ttcgggagat tccccacgtc
      601 gtaggggaat ggtaatatca tgaacaaagt gtttggtcca cgaactgcga attgcaggag
      661 tcgatacgac aatcagttgt gggccatcgg ccagtctcga tgtagcgcag tgaagacaag
      721 cctccgatat ttcggagaac actccgaaat atcggatcgc caggtcaggg cggaacatga
      781 acgacgacat gggcggcctc ccggctcccg aaatggccaa cgatttcgga gcgacaagac
      841 ccgacgaagg tccccggctc ccgggcgaat cgcgcctctc ccgcgtggcc tcccgcctgt
      901 cgagcgcttc catcgccgac ctggatcaga cgatcgtcga cggcctctgc caggccgtcg
      961 aactcctcga cctcgaccga agcgccgtgt ggcaattcac cgagtccggt gatgacctcg
     1021 tgtacaccca tgtctgggac cgtccgggct cagccgcgat gcgccatccc ttctccgtcg
     1081 gcaccctgtt cccctggcat cgcgcggaaa ttcgcgaaga ccgtgccatc tggaacgaac
     1141 gggttgacga cgtcccctcc gccgtcgacc gacaaagcct cctgaaactc gagaccgccg
     1201 ccaacgccgt agtgccgatg cgcagtgacg ggaccctcat cggggcgctt tcgttcgact
     1261 cctgccgtcg atccgtccac tggagtccgg aggtgcgcga ttgcatgcga gtattggcgt
     1321 cactcttcag ccagacgatt gcccgcgctc gaaaggaccg ccaacttggc ctggccctcg
     1381 acgagatcgc gcgactccgg aaccaggtcg tccgtgagcg catcggcgcc gggacgctgt
     1441 catcgtcgcc gcagccggaa tccgggccat cgcccgagcc tctccgtttg agctacgtgg
     1501 agagcgccca tatccggcat gtcctcgaac gctcggggtg gcggatccgc ggcgcgaacg
     1561 gcgccgccga gatgctcggg atgaagccca cgacgctcga gagccggatg gtcaagttgg
     1621 gaatccaccg ccccccgaga cggatcgttc gcgctccggg atattgactg ccccggttga
     1681 ccggtgcgcg gctccccgtg cctttgtgta tcctcccgcc ccgaggcagc ccgacatgac
     1741 gccggagctg gacaggagac aagctgaatg tcaccgactg gtagtttcgg aacgattcgt
     1801 tcgctgaccc tcgccgtcac cggttccgtc ctcggtcttg cgctcgtagc cgtggcgccg
     1861 ggccgggccg ttgcccagga ccccgcgatg acctccacca tcgacagcac gatcgtggcc
//
LOCUS       DASVOD010000670         7585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_878633, whole genome shotgun sequence.
ACCESSION   DASVOD010000670 DASVOD010000000
VERSION     DASVOD010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7585
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878633"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2469
                     /locus_tag="VF720_14345"
     CDS             <1..2469
                     /locus_tag="VF720_14345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX7880591.1"
                     /translation="PSRLDPRLRSFMRQYEEARGARLAPAGIKAASAAMFGAGTTALS
                     AQGRLTVDCLIKIDNESALDRLAELGIEPRTRAGNILTVDIPVDELVYLEDLVGIRQV
                     EMSRPLKPLLDVSRTLTNTVQVQGGPAPPWTGFTGQNVVVGVIDTGLDLTHGDFRNGD
                     NTTRIDGVWDQTNPSGPAPAGFGYGREWTEAQINAGTATEADNDGHGTHVLGIAAGDG
                     SETGNGQPAYQYVGMAPEADIIGVKTNFTTGGVADGVAYIFNKAGVRDAVVNLSLGTQ
                     DGPHDGTSLFDQGLSMLIGPGRSIVAAAGNEGGAGAHARLAVMATGDSAVFTFTVPSY
                     TPNGGGTTQSPNDFVVIDGWYEGTDNFSFRVESPTGKRTSEVLIGANNNGGRCLPTTG
                     GDGRCYVENAQTEPDNNDREIYIELTEGTISATACGVPKSGVWKIIAYKKANSTGPGT
                     IDFWIANYLLGSGGAFPEFAQGASDSYLVGSPASAVGIISVGAFISKRQWVSQSGGRM
                     YTGLTDDDLGTIAPFSSPGPLRNEVLAPVISAPGMGIASTRSGSASIETIWNMVDGKH
                     TINQGTSQASPHVAGVVALIFEAFPHETPINVMNRLTHTATRDAKTTDQPNAIYGYGK
                     LNAFGALSFDTPVFLSTFEALPHEGGVALTWRVESDAPFVGFHVERADEKAGPFVRMT
                     SALLGGGPSFEWRDDSTEPGRDYWYRLEAIEVDGSLTRFGPLAARAGAPRLSLAQNGP
                     NPFAATTLIEYALPGAMPVSVRVFDLAGRLVRTLVNGMQPAGAGSVSWDGLDESGNGV
                     PNGAYFYRIESAAGSLSKKMILSR"
     gene            2471..3976
                     /locus_tag="VF720_14350"
     CDS             2471..3976
                     /locus_tag="VF720_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013942184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HEX7880592.1"
                     /translation="MTDGSLTPIAGVAIAAWLAVGACLFIYGIHAYLMTWLFRRHGRS
                     TPAPRRDAAGHGDRPIVTVQLPLYNERYVAERLIEAVTALDWPRHRLEIQVLDDSTDD
                     TAERVARMVAGRRAAGFRIAHLTRAHRTGFKGGALREGLAAAAGEFVAVFDADFLPPP
                     DFLLATIPHFNDPAVGMVQTRWEHLNERGSLLTRVQAVALDGHFLVEQTARNRSGAWI
                     NFNGTAGIWRRRAILDAGDWQDDTLTEDLDLSYRAQMAGWRFVFLPGVACPAELPVDI
                     DGFKGQQFRWAKGSVQTAMKLLPRILRSNAGPWVKLQALIHLTNHAVYPLLLALALLS
                     LPILVLHAHHPELRPFVTVATWFIVASVGHPVLYLTAQERLRNRWKSRIVLMPALIAI
                     GMGIAVNNTRAVIEALAGQASGFNRTPKYDLCRPGDSWRDKRYRVPPTAWPLVELAFG
                     AWSATALGYAAFHGQWLAMPFLALYTAGFLAVGSASLARAVDTRPPLADAA"
     gene            3973..4974
                     /locus_tag="VF720_14355"
     CDS             3973..4974
                     /locus_tag="VF720_14355"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7880593.1"
                     /translation="MTDTIRKFLDERLPALFDARPADIDPWFFDYFGELARPEARRRY
                     ARAVAADLQLARFEPAGKVMLDAGSGFGITLLCFAALRPRAAFGLEAFRPMAETSSRL
                     RRQLAPAFPAVVLRGSVTAFPLPRASVDFIYCNEALSHFLDRDAFYDESARVLRPGGI
                     LMICDGNNGLNARTVARVHQIWKHFEEGPPMDDLHGHRIEVPYRDRRREMIARVITGQ
                     DASTLDRLAWGTFGRHGAGITDEARRLLAEGNLPAAPPVVDRSPVDPVKGDHIESLVD
                     ARDLEARLSSRGFRVTTWAHFGGARSPLVAGVNRLLRAASHFTLPWARSVKIVATRR"
     gene            5222..5848
                     /locus_tag="VF720_14360"
     CDS             5222..5848
                     /locus_tag="VF720_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880594.1"
                     /translation="MTTDRLAASRTRARSLAFIVTSLVLLAAGGCGRDPGATAPDSAS
                     TSGATADGTLDMAALPRFESVGSGSQGDPWYLVTMSTVKPLQLKTVAGSRYSLLFLPG
                     SVVGKSIDVTIHERHPDIIDVQFGPHGTQFGVPVTLTINYAGTVLDPAHPNFKPGPIQ
                     FYWLDPLPLLWLPIPGVDNPLTKTYTVTLSHFSRYTLARTPPSGTADW"
     gene            5889..7400
                     /locus_tag="VF720_14365"
     CDS             5889..7400
                     /locus_tag="VF720_14365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880595.1"
                     /translation="MSTLAVPAPRSAAPLPIWAALYVALVNALGLAAILPVLALLPDA
                     NVGIIAAWSGACLAGELLNMPTPTRRGQISMATSFHLAMALVLSPVEFLPVVWISRLA
                     VKGLIQRQAWYRAMFNAAQVAVSVCVAWLLWRSIQPAGLNSLAGGDAGSIGGAAGASP
                     GFLAAAFGYYIANTFSVSGIIALTGGGRLLDAWRINYGYPVEILGTVALLLLAPAVAS
                     SVAVFGPAGLALLVAPVAAMHAAGHSFRRQEERQQERIAAERLAAKSELSTQVGFEMT
                     EALTTISGNIQLLDRHWPNVGEAEFKRRVEEIRRTSDRISVLSRGLTEFSRGGFAPDW
                     IDPATVIGRSLAFLKTEGRLAHVRVETVLDREIREVFVDAPQMQQALINLLRNSAAAL
                     EAARTNPGWIWIRVRRDRRRQAVEFVIEDNGPGLPAGSEWQVFEPGFTRQPGRSGFGL
                     STVHRIVRNHDGTVEASTRPGGGARIGIVLPAPAHRRASSDQSSEARPPLHMP"
     gene            complement(7361..>7585)
                     /locus_tag="VF720_14370"
     CDS             complement(7361..>7585)
                     /locus_tag="VF720_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880596.1"
                     /translation="REASVRCHAESRARRQAEGHVESIALLFKAWPRQTWRDLGRLVR
                     EGNAVSWPRAAVYRLAQFYGMWRGGRASED"
BASE COUNT         1181 a         2686 c         2502 g         1216 t
ORIGIN      
        1 ccatcgcggc tcgacccgcg cctgcggagc ttcatgcgcc agtacgagga ggccaggggt
       61 gcccgattgg cgcccgccgg catcaaggcg gccagcgccg ccatgttcgg agccgggacg
      121 accgcgctct cggcccaggg acgcctgacc gtcgattgcc tcatcaagat cgacaacgaa
      181 tcggcgctcg accgcctggc cgaactcggc atcgagcccc gcacccgggc cggcaacatc
      241 ctcaccgtgg acatcccggt cgatgagctc gtctacctcg aggacctcgt cggaatccgt
      301 caggtcgaga tgagccggcc gctcaagccg ctgctcgacg tcagccgcac gctgaccaac
      361 acggtgcagg tgcagggagg gcccgcgcct ccctggaccg ggttcaccgg tcagaatgtc
      421 gtggtcggcg tcatcgacac cggcctcgat ctcacccacg gcgatttccg gaacggcgac
      481 aacaccacgc gcatcgacgg cgtgtgggac cagacgaatc cttcgggtcc ggccccggcc
      541 gggttcgggt acgggcgcga gtggacggag gcgcagatca atgccggcac cgccaccgaa
      601 gccgacaacg acggccacgg cacgcacgtc ctcggcatcg cggccggcga cggctcggaa
      661 accgggaacg gccagccagc ctatcagtac gtcggcatgg cgcccgaggc ggacatcatc
      721 ggggtcaaga ccaatttcac caccggcggc gtggccgacg gcgtggccta catcttcaac
      781 aaggcaggcg tgcgcgacgc ggtcgtcaac ctctcgctcg gtacgcagga cggaccccac
      841 gacggtacat cgctcttcga ccagggattg tcgatgctga tcgggccggg acggagcatc
      901 gtggcggcgg cggggaacga agggggcgcc ggggcgcacg cgcggctggc cgtgatggcc
      961 accggcgaca gcgccgtctt caccttcacg gtccccagct acacgccgaa cggcgggggc
     1021 accacgcaat cgcccaacga cttcgtggtc atcgacgggt ggtacgaggg aaccgacaac
     1081 ttctccttcc gcgtggaatc cccgaccggg aagcgaacga gcgaagtgct gatcggggcc
     1141 aacaacaacg gcgggcgctg cctcccgacg accggcggcg acgggcgctg ctacgtggag
     1201 aatgcccaga ccgagcccga caacaacgac cgtgagatct acatcgagct gaccgagggg
     1261 acaatcagtg ccaccgcctg cggcgtcccc aagtccggcg tctggaagat catcgcctac
     1321 aagaaggcca actccaccgg gccgggaacc atcgatttct ggatcgccaa ctacctgctc
     1381 ggctcggggg gtgccttccc ggaatttgcc cagggagcca gcgattcgta cctggtcggc
     1441 tcgccggcct cggccgtggg catcatcagc gtgggtgcgt tcatctcgaa gcggcagtgg
     1501 gtttcccagt cgggcggacg gatgtacacc ggcctgaccg atgacgatct cgggacgatc
     1561 gcacccttct cgagccccgg accgctccgg aacgaggttc tggcgcccgt catttccgcc
     1621 ccgggcatgg ggatcgcctc cacccggagc ggcagcgcct cgatcgagac gatctggaac
     1681 atggtggatg gcaagcacac catcaaccag ggaacgagcc aggcctcgcc gcacgtggcg
     1741 ggggtggtgg cgctcatctt cgaggcgttc ccgcatgaaa cgccgatcaa cgtgatgaac
     1801 cggttgacgc acaccgcaac acgcgacgcc aagactaccg accagccgaa cgcgatctac
     1861 ggctacggca agctgaatgc gttcggcgcg ctgagcttcg acacgccggt cttcctctcg
     1921 acgttcgagg ccctgccgca cgagggaggc gtggcgctca cgtggcgggt ggagagtgac
     1981 gcgccgttcg tgggcttcca cgtggaacgc gccgatgaaa aggccggccc gttcgtacgg
     2041 atgacctccg cgctgctcgg cggcggcccc tccttcgaat ggcgggacga ctcgaccgaa
     2101 ccggggcgcg actactggta tcgcctcgag gcgatcgagg tggatggttc gctgacccgc
     2161 ttcggtccgc tggccgcacg ggccggcgct cctcggctct cgctggccca gaacggcccg
     2221 aacccgtttg ccgccacaac cctcatcgag tacgccctgc cgggcgcgat gccggtttcg
     2281 gtccgcgtgt tcgacctggc cggccgactc gtccgcacgc tggtcaatgg catgcagccg
     2341 gccggcgccg ggagcgtgag ctgggacggg ctggacgaat cggggaacgg cgtgccgaac
     2401 ggcgcctact tctatcggat cgaatccgcc gcggggagcc tctcgaagaa gatgatcctg
     2461 tcgaggtgag atgaccgacg gttcgctgac tccgatcgcc ggcgtggcaa ttgcggcctg
     2521 gctggcggtc ggggcctgcc tgttcatcta cggcatccat gcctatctca tgacgtggct
     2581 tttccgtcgc cacggtcgtt cgactccggc accgcgccgg gacgcggcgg gtcacggcga
     2641 ccggccgatc gtcacggttc aattgccgct ctacaacgag cgctacgttg ccgagcggct
     2701 catcgaggcc gtgacggccc tggactggcc gcgccaccgg ctcgaaatcc aggtgctgga
     2761 tgactcgacc gacgacaccg ccgagcgagt ggcccggatg gtggccgggc gacgggcggc
     2821 gggattccgc atcgcgcacc tcacccgggc ccaccggacc ggattcaagg gaggcgcgtt
     2881 gcgtgaaggc ctggccgccg ccgccggtga gttcgtggcc gtcttcgatg ccgatttcct
     2941 tccgccgccc gacttcctgc tcgccaccat ccctcacttc aacgacccgg cggtcggcat
     3001 ggtgcagacg cgatgggagc atctcaacga gcgcggttcg ctcctgacgc gcgtgcaggc
     3061 ggtggccctg gatggccatt tcctggtcga gcagacggcg cgaaaccgga gcggtgcctg
     3121 gatcaacttc aatgggacgg ccggcatctg gcggcgccgg gccattctcg acgccggtga
     3181 ctggcaggac gacacgctca ccgaagacct tgacctttcg taccgggcgc agatggccgg
     3241 ctggcgtttc gtcttcctcc ccggcgtcgc ctgcccggcc gagctccctg tcgatatcga
     3301 tggcttcaag ggtcagcagt tccggtgggc caagggttcg gtgcagacgg ccatgaagct
     3361 cctcccgcgg atccttcgct ccaacgcggg cccctgggtc aagctgcagg ccttgatcca
     3421 cctgacgaac catgcggtct acccgctgct gctcgccctg gcgctcctgt ccctgccgat
     3481 cctcgtgctc cacgcgcatc acccggagct caggccgttc gtgacggtgg ccacgtggtt
     3541 catcgtggcc agcgtaggcc accctgtgct ctacctcacg gcgcaggaac gactgcggaa
     3601 ccggtggaag agccggatcg tcctgatgcc ggccctcatc gcgatcggca tggggatcgc
     3661 cgtgaacaac acgcgggccg tgatcgaggc actggccggg caggcatcgg gcttcaaccg
     3721 gacccccaag tacgaccttt gccggccagg cgattcctgg cgcgacaagc ggtaccgcgt
     3781 gccgccgacc gcctggcccc tggtcgaact ggccttcggc gcctggtcgg ccaccgcgct
     3841 cgggtacgcc gccttccatg gccagtggct ggccatgccc ttcctggccc tgtacaccgc
     3901 gggcttcctg gcggtcggct cggcgtcact ggcccgcgca gtcgatacgc gccccccgct
     3961 cgcggacgcc gcgtgaccga caccatccgg aagttcctcg acgagaggct ccccgcgctc
     4021 ttcgacgcgc ggccggccga catcgatccc tggttcttcg attacttcgg cgaactcgcg
     4081 cggccggaag cgcgacgtcg ctacgcccgc gccgtggcgg ccgaccttca gttggcccgc
     4141 ttcgagccgg ccgggaaggt gatgctcgac gcgggaagcg gattcggcat cacgctcctc
     4201 tgcttcgcgg cgctccgtcc gcgggcggcg ttcggtctcg aagccttccg gcccatggcc
     4261 gaaacgtcct cacggctgcg ccggcaactg gccccggcgt tccccgcggt tgtgctgcgc
     4321 ggctcggtga cggcgtttcc gctgccgcgg gcgtcggtcg acttcatcta ctgcaacgag
     4381 gcactcagcc acttcctgga tcgcgacgcg ttctacgacg agtcggcccg ggtgctcagg
     4441 ccgggcggga tcctcatgat ctgcgacggg aacaatggcc tgaacgcccg cacggtggcc
     4501 cgcgtgcacc agatctggaa gcacttcgag gaagggcccc cgatggacga cctccacgga
     4561 caccggatcg aggtgccgta tcgcgatcgg cgacgcgaga tgatcgcccg ggtcatcacc
     4621 ggccaggacg cgtccacgct cgaccggctg gcgtggggca ccttcggccg ccacggtgcc
     4681 ggcatcaccg acgaagcccg ccgcctcctc gcggagggga atctccccgc ggcaccaccg
     4741 gtcgtcgatc gatcgcccgt ggatcccgtg aagggggatc acatcgagag cctcgtcgac
     4801 gcgcgcgacc tggaagcgcg gctttcgtcc cggggtttcc gcgtgaccac gtgggcccat
     4861 ttcggcggcg cgcgaagccc gctggtggcg ggggtgaacc ggctgctccg ggctgcctcc
     4921 catttcacgc ttccctgggc tcgctcggtc aagatcgtgg ccacacggcg ctgatcatcg
     4981 ccggctgatt tcagccgcat gcgggtgggc tgacgcaatc gcccggatcg ccccctttcc
     5041 aacgatcgcg gaagattttc ctcgagggtg aagcgattgc ggcggcatgc cttgaccgga
     5101 acgcccgtcg caggacaggc acgcttcacg aggcacgctt cctgctcgat accgcctcga
     5161 ctccggagct gatgtcccgc ccgacgcggc gcgtcacccg gcaccgacct ggaggtgctc
     5221 gatgaccacc gaccgactcg cagcatcgag gacgcgggcg cgaagcctgg ccttcatcgt
     5281 cacctcgctg gttctgctgg cggccggagg ctgcggccgc gaccctggcg ccacggcgcc
     5341 cgactctgcg tccacgtcag gcgcaaccgc ggacgggacg ctcgacatgg cggcgcttcc
     5401 ccgcttcgaa tcagtgggat ccggttccca gggcgatccc tggtacctcg tgaccatgtc
     5461 gaccgtgaag ccgcttcagc tcaagaccgt ggccggcagc cgctactcgc tcctcttcct
     5521 gccggggagc gtggtgggca agtcgatcga cgtcacgatc catgagcggc atcccgacat
     5581 catcgacgtc cagttcgggc cgcatggaac acagttcggc gtgccggtca ccctcacgat
     5641 caactacgcc ggcaccgtgc tcgacccggc ccatcccaac ttcaagccgg ggccgatcca
     5701 gttctactgg ctcgatccgc tcccgctgct ctggttgccg atccccggcg tcgataaccc
     5761 gctcacgaag acgtacaccg tcacgctcag ccacttctcg cgctacacgc tcgcacgcac
     5821 gccgccgtcg ggcacggccg actggtgatc cgaaaccctt tgactcgaac ggaaacgatg
     5881 gagcgagcat gagcacactg gcggtcccgg cacctcgaag cgcggcaccc ttgccgatct
     5941 gggccgcgct ttacgtggcc ctggtcaacg cgcttggcct ggcggccatc ctgccagtgc
     6001 tcgccctgtt gccggacgcc aacgtcggga tcatcgccgc ctggtcggga gcctgcctgg
     6061 ccggggaact cctcaacatg cccacgccca cccgccgggg tcagatctcg atggccacgt
     6121 cgtttcacct cgcgatggcg ctggtgctct cgccggtcga gttccttccc gtggtgtgga
     6181 taagccggct ggccgtgaag gggctcatcc agcggcaagc ctggtaccgc gccatgttca
     6241 acgccgccca ggtggccgtt tcggtgtgcg ttgcctggct tctctggcga tcgatccaac
     6301 cggcgggcct caactcgctc gcgggcggcg acgccggctc gatcggcggg gcggccggag
     6361 cgagtcctgg cttcctggcc gcggcgttcg gctactacat tgccaacacg ttttcggtca
     6421 gcggcatcat cgcgctgacg gggggcggtc gtctcctcga tgcctggcgc atcaactacg
     6481 gctacccggt cgagatcctc ggcacggtgg cgctccttct gctcgcaccc gccgtggctt
     6541 cgagcgtggc ggttttcgga ccggccggcc tcgcgctgct ggtggcgccg gttgcggcca
     6601 tgcatgccgc cggacactcg tttcgccggc aggaagagcg gcagcaggag cggatcgcgg
     6661 cggagcggct cgcggccaag tccgagctct ccacccaggt gggcttcgag atgaccgagg
     6721 cgctcaccac catctcgggg aacatccagc ttctcgatcg ccactggccc aacgtcgggg
     6781 aagcggaatt caagaggcgg gtggaggaaa tccgccgcac cagcgatcgc atcagtgtgt
     6841 tgagccgcgg cctcaccgag ttctcccgtg gcgggttcgc tcccgactgg atcgatcctg
     6901 ccacggtgat cgggcgtagc ctggccttcc tcaagactga gggacgcctg gcccacgtac
     6961 gcgtggagac ggtcctcgac agggagatcc gcgaagtatt cgtcgacgct ccccagatgc
     7021 agcaggcgct catcaacctg ctccgcaact cggccgcggc actcgaagcg gcccggacga
     7081 atcccggctg gatatggatc cgcgtgcgac gcgaccgtcg gaggcaggcg gtggagttcg
     7141 tgatcgagga caacggccct ggactccccg cggggagcga atggcaggtc ttcgaaccgg
     7201 gattcacgcg ccagccgggt cgatccggct tcggactctc cacggttcat cgcatcgtgc
     7261 gcaatcacga cggaacagtg gaagcctcaa cccggccggg tggcggcgcg cgcatcggca
     7321 tcgtgctgcc cgccccggca caccgccgcg cctcctccga tcaatcttcc gaggcgcggc
     7381 cgcccctcca catgccgtag aactgcgcga ggcggtagac ggcggcgcgt ggccagctca
     7441 ccgcattccc ctcgcgcacg agccggccga ggtcccgcca tgtctgccgc ggccaggcct
     7501 tgaacagaag ggcaatgctc tcgacatgtc cctccgcctg gcgacgtgcc cgcgactcgg
     7561 cgtggcagcg aacggaggcc tcccg
//
LOCUS       DASVOD010000671         2117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_408026, whole genome shotgun sequence.
ACCESSION   DASVOD010000671 DASVOD010000000
VERSION     DASVOD010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408026"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2117
                     /locus_tag="VF720_14375"
     CDS             <1..>2117
                     /locus_tag="VF720_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880597.1"
                     /translation="NSITINNTNGVDIAGGGVTAKTSLTLTAGNVRTTATNLLTLATT
                     ITAPPTGSATSHVDGPLGIQVQVTSASTRTFAVGASGAWRPLVIKLLNTGGVLRTFTA
                     QVLPGPTGGTPQNPLQSLTTTRYWRIGGSANLNNTARVNLAFGLDDGVGTLANARVAQ
                     ATTSNGTYSNLGGATTGTSSAGTVESTANLSPGGDYFAIASTGSLAATWDGGAGTSNW
                     GDAANWNPDGVPGVSANVTLAPGSPISINVNGTFPLNNLNVGANTTLSLLTGTLNVAG
                     AYFQSGGSVDAGSGALNVTSTLSLAGGSLSTAAATITGTGVVTMSGGTLALNGTGRLL
                     ATSDFTQSGGTIGFGSGTLEVKGTFSRTAGTFNAGTGTTIFSGTNTQVLGGGVSYYNL
                     ILRNGGNGKPKRFTSEAFYTVNNDFTVENTAQVSLSSADATDFTIWGNLNYSGLPSGT
                     NISNFKIGLRGAGKFIQGATGSPQPAPIETEAHRVKVPAGEVREVNLLTDPARAVVLE
                     GTGHGKRRLRLDNTFEKKRGEVERLLAAARPGDRLVINLDDRTIMLDLAPIDELASAA
                     SELNMPVSVEYGAAYTLGGNTSVAASRLLTIAGRLECGTSTISGAGGISLSGTLATMT
                     ASATGLGGTVVTSGADTYVSGSIVEYNAAGNQTISAVNHPAASLIRTAGSGTKTLDAD
                     KTITGDSGALLTEGALYVGAGTT"
BASE COUNT          405 a          731 c          683 g          298 t
ORIGIN      
        1 aacagcatca cgatcaacaa caccaacggc gtcgacatcg ccggcggggg ggttacggca
       61 aagacgagcc tcaccctgac ggccggcaac gtccgcacga cagccacgaa tctcttgaca
      121 ctggccacca cgatcaccgc cccgccgacg gggtcggcca ccagtcatgt cgatggtccc
      181 ctcgggattc aggtccaggt gacgagcgcg tcgacgcgca cctttgccgt cggtgcgagc
      241 ggcgcgtggc gaccgctcgt catcaagctt ctgaacaccg gtggggtcct tcggaccttc
      301 accgcccagg tgcttcccgg cccgaccggt ggaacgccac agaatccgct gcagtcactg
      361 accacgaccc gctattggcg gatcgggggg agcgccaatc tcaacaacac cgcgcgcgtg
      421 aacctcgcct tcggcctcga tgacggcgtg ggaacgctgg cgaatgcccg cgtggcacag
      481 gccacgacat cgaacggcac ctattcgaac ctcggcgggg caacgaccgg aacctcatcc
      541 gccggcaccg tcgagtcgac ggccaatctc tcccccggag gcgattactt cgccatcgcc
      601 agcacgggga gcctggccgc cacctgggac ggcggggccg ggaccagtaa ttggggggac
      661 gcggccaact ggaacccgga cggcgtcccg ggcgtctcgg ccaacgtgac cctcgcgccg
      721 ggttcgccca tcagcatcaa cgtcaacggg acatttccgc tgaacaacct gaacgtcggc
      781 gccaacacca cgctgtcact cctgaccggc acgctgaacg tggcaggggc ctatttccag
      841 tccggcggct cggtcgatgc cgggagcggg gccctcaatg tcaccagtac gctgagcctg
      901 gcggggggat ccctgtcaac cgccgccgcc acgatcaccg gcaccggagt cgtcaccatg
      961 tcaggcggga cgctcgccct gaacggcacc ggtcgtctcc tggccaccag cgacttcacg
     1021 cagtccggcg ggacgatcgg gttcggatcg ggaacgctcg aggtcaaggg aaccttcagc
     1081 cggaccgccg gaacgttcaa cgcgggcacc ggtacgacga tcttctccgg gaccaacacc
     1141 caggtccttg gcggcggcgt gagctactac aacctcatcc ttcggaacgg gggcaacggc
     1201 aagccgaagc ggttcacgtc ggaagcgttt tacaccgtga acaacgactt cacggtcgag
     1261 aatacggcgc aggtatcgct gagcagcgcc gacgccaccg acttcaccat ctgggggaac
     1321 ctgaactatt ccgggctccc cagcggcacg aacatctcga acttcaagat cggcctgcgc
     1381 ggggccggga aattcatcca gggagccacg ggctccccac agcccgcgcc gatcgaaacg
     1441 gaggcccacc gggtcaaggt gcccgccggc gaggtccgcg aggtcaacct cctgaccgat
     1501 ccggcccggg cggtcgtgct cgaaggcacc gggcacggaa agcgccgtct ccgcctcgac
     1561 aatacgttcg agaagaagcg gggagaagtc gagcggctgc tggcggccgc caggcccggt
     1621 gaccgtctcg tcatcaacct ggacgaccgg accatcatgc tcgacctggc gccgatcgat
     1681 gaacttgcca gcgcggcctc cgagctcaac atgccggtct ccgtcgagta cggagccgcc
     1741 tacacgctcg gggggaacac gtcggtggcg gcgagccgac tgctcaccat cgcaggaagg
     1801 ctcgagtgcg gaacgagcac gatttccggc gccggcggga tttccctcag cgggaccctg
     1861 gccacgatga ccgcgagcgc cacggggctt ggtggaacgg tggtcacgag cggggccgac
     1921 acgtacgtga gtggctccat cgtcgaatac aacgcagccg ggaaccagac gatcagcgcc
     1981 gtgaatcatc ctgctgcctc gctgatccgg acggccggga gcgggacgaa gacgctcgac
     2041 gccgacaaga ccattacagg tgattcgggc gcgttgctga cggaaggggc cctctacgtc
     2101 ggggccggga cgacgtt
//
LOCUS       DASVOD010000672         9567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_773581, whole genome shotgun sequence.
ACCESSION   DASVOD010000672 DASVOD010000000
VERSION     DASVOD010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9567
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773581"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VF720_14380"
     CDS             complement(<1..227)
                     /locus_tag="VF720_14380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880598.1"
                     /translation="MAGRRLLAVQLKRLGDLVLARPALQALASSGDRVTLLTSAPFDE
                     AVRIDPWLDAIHLHPAGWAASLRFGAGLARER"
     gene            complement(231..2159)
                     /locus_tag="VF720_14385"
     CDS             complement(231..2159)
                     /locus_tag="VF720_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880599.1"
                     /translation="MNPNRRLLAYLKPEWRTLALGLVCMAGYALLSGFSIGMIKPIVD
                     GLFAADAAHAVPLGTPPAAVPPPGIGVVLGETFGHLRTMSLDGARQSLTSGVNAILAS
                     APRRQVLQFVCIAALILILLRNTFDYLRKILFTRLEQRATEAIRNDLYARVIHLPLSA
                     FDRNHSGNLISRVINDVESIKSFTVSGFTQVLHNGLLVIVFLAITFKADARLAMATFV
                     ILPPLMFLLGRLAVKLKKHSGRAQQRLADLQQHLQETVHSVRVVKAFAQEDTERDRFR
                     KATDRYRRTVTRLLSIDLLAAPLSEFWAVSVGVVVLWYGGLQVLDPSSGLTGGEFSAF
                     LIAMFMLMHPLKEVSGALGKIQRGRVAAERVFELMDLPSEPLDDGGARIDRFTGSVRF
                     REVGFSYDGAKPVLKDIAFEARQGETVALVGPSGAGKTTLVDLIPRFYEPTEGVIEVD
                     GRDTRLLNLKDLRRLMGIVTQETILFDDTVAANIAYGRPDASRGEVEAAARAANAHDF
                     IAAMPLGYDAPIGESGQLLSGGQRQRLAIARAILQNPPILIFDEATSSLDTESEALVQ
                     EAIDHLLADRTTFVIAHRLSTVTRADRILVIDGGRIIEDGTHGALLARGGAYSRLYRR
                     QFRDDEREAILSPAGEPA"
     gene            complement(2234..7063)
                     /locus_tag="VF720_14390"
     CDS             complement(2234..7063)
                     /locus_tag="VF720_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX7880600.1"
                     /translation="MTRPFLLSALTLLAMVGVGGAAEPVRLVQTVEVSATDVLIRRNS
                     NVDFITLPGGAIGTEEGMPDLPVKPLTFRLPEEMRIASVDVRVLETQALRGEFHPATI
                     RTRNSNDEFLPLSFSATTRPTDGEGFYPATLAEPGASGRMRGRALGGVVYRPFRWRES
                     DGRLEIVTRAELVLTLEPETRDRKDDFLPLRREPWADAIFDREVEDLVVNRSPDVAGR
                     TVGAEISPLLNSGAPFAPTFRPSVNGTPVEMVIITNETQQSQYQRLADWKTRAGTPCV
                     VRTVNWIKANYPQGVDTQETIRNFIRDAAQKWGTVWVLLGGDTDVIPTRYGKTQFFSG
                     EEIPTDLYFQCVDGTWNADGDAEFGEGSIPPDPVDSVDLYPEVWIGRLPTVTLAQATT
                     LVNKILAYEVTPLRNGYQDDYLALAEVLFPQTWNPGDTILFDGASIAEEGISHLPGNY
                     RVVKLYENCPNASWPTCILEQKIPTVLDSLNAGFGFVHHLGHGYINTMSVGKADQALT
                     NADADACNNGNETFFLYAINCTSSAIDFNCIAERYMLNSHGGSVASVGSTRFDFPSTG
                     WSYQTEYYQLVFEDGIHELGHAASACKLPFVPLAQQDNTHRWTQYTQIYFGDPSMPLF
                     TQPPADLAVTHAATHALGAGTFTVTVNSGGNPLQNARVCLNKVNDEYAVGLTNGSGQI
                     MLPFVPDSTGTTQLTVVADNHVPYQANITVTTPVAPYLYAADQLIDDDGSGSSTGNGD
                     QKIDAGETIELSLPVKNRGGASSPITATVISLSPYVTVIDGTSSYAAAGAGAQVNPGD
                     PLVLSISRTAPDRTEPKIQVTWQAGATSIVEEVVLYIHAPVFQWFRQFPRDTTGNGNG
                     NSVFAPNEDVTLRVELRNAGLGQARVVNAVLRSTDPSIVVNDSTVAFGTLAGGALAIS
                     RPSDTFRFQMTDTTGFAAGNKKLRLSIYDHYSPSTVLASWLIDARSPVGTLGGLTARG
                     FETSIAVTFTPVTSADLYGYNVYRSSAAVGPFSRINQNTTMRTAYYNDEGLPPLTVFY
                     YKVAAQDSSGNEGPLSIAFSASTTMPLHDEFPVELEAATNASVTLADLDYDGDLEILA
                     GASEVYALKPDGTELFDGDNDIRTLGVLTNTGKPGFWNAPAVGDVDKDGSPEVAAVSW
                     LASMYLWNEFGEVEPGWPKNINAQALGDPNPLSSVTMGDIDNNGDLELFVMCGRLLYG
                     FHHDGTEIVDGDANPSTVGVIKVINTAYNYGTPALADMNGDGRPEVIVGMRDKKLHVF
                     NPVNWQELPGFPFTTNGNSGAGGEITSSPAVADIDNDGQLEIVFGASDSRVYALNGDG
                     TAAAGWPQGIQLNEDLDSSPALGDITGDGIPDVVVGASNGRVVAWRNNGTLIAGWPVF
                     IRDALNANVPVRSSPNLVDIDNNGVPEIIIGDQIGRLHGFYANGTLLPGFPIQTGNLI
                     EGGPAAWDLDGDGLTEIVAESYDQKVYQWDTPWTFNKLASPWPMFHHDPRHTGRLGEP
                     LYYQTGVPETPDAGTGLKPFLLAQNRPNPFNPNTTIGWRIQSASRGNELVPVRIEVYS
                     PTGRLVRLLVDRPMPVGEYEVNWDGNDQEGVPVGSGVYYYRLVSKDGSESRKMVLVR"
     gene            7437..7559
                     /locus_tag="VF720_14395"
     CDS             7437..7559
                     /locus_tag="VF720_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009733468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880601.1"
                     /translation="MASERTREIKRRRKRREERIKSRRKESIAKGKKGATRPAR"
     gene            7649..8086
                     /locus_tag="VF720_14400"
     CDS             7649..8086
                     /locus_tag="VF720_14400"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX7880602.1"
                     /translation="MPASRPDGPRLFTLEEANALIPRLETALSEMAAFRAELKLLRKD
                     IEVLSLIAASAGGNENPDAAELKNKRRRYRSVASEIERIGTGLEEAGCLVKHPDEGLV
                     DFFHLRGDRLVFLCWKVGEKRVEHWHPLSGGYATRKSLGQPES"
     gene            8248..9036
                     /gene="radC"
                     /locus_tag="VF720_14405"
     CDS             8248..9036
                     /gene="radC"
                     /locus_tag="VF720_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX7880603.1"
                     /translation="MPIADLYPAPPANLTPFDDEEEDEAVELPLAAPPPHAPVPGAAA
                     LRSRWDRGPRERLLREGLGALNDAELLALMFGTGSRGAPAVQLAERLLVARGLTGLAA
                     IDPVDLCVERGIGPARSAQIAACFELGRRLFGRDEGAVLIKGPEDVYEATTDLRGARK
                     EHFVTFHLNARNQVIARETVSIGSLNASIVHPREVYEPAVRNSAASIILVHNHPSGET
                     DPSDDDLALTRRLVQVGDLLGIAIVDHIIVGRRGFTSFKQQGYI"
     gene            9124..>9567
                     /locus_tag="VF720_14410"
     CDS             9124..>9567
                     /locus_tag="VF720_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX7880604.1"
                     /translation="MTTLTAEQVSHHLRNVRDRPILVLGDVMLDRYLWGNVHRISPEA
                     PVPIVDVESETVRLGGAANVAHNVSALGARPILVAVAGEDHPASLLRAELAARGLSAA
                     GLVVDAGRPTSIKTRIVARHQQVVRADQESREEIRGPVEERVLAVV"
BASE COUNT         1590 a         3073 c         3331 g         1573 t
ORIGIN      
        1 cgctcgcgcg ccagtccggc cccgaagcga agcgaggcgg cccacccggc cgggtggagg
       61 tgaatggcgt cgagccaggg atcgatgcga accgcctcat cgaaaggtgc gctcgtgagg
      121 agcgtcaccc gatcacccga cgacgccagg gcctgtagcg ccgggcgtgc caggaccagg
      181 tcgcccagcc gcttcaactg cacggccaac aggcgccggc cggccacgcg tcaggccggt
      241 tcgccggcgg gggagaggat cgcctcccgc tcgtcgtcgc ggaactggcg ccggtagaga
      301 cggctgtagg cgccaccccg ggccaggagt gcgccgtgcg tgccgtcctc gatgatccgt
      361 ccgccgtcga tcacgaggat gcggtcggcc cgcgtcaccg tggagaggcg atgcgcgatg
      421 acgaacgtgg tccggtcggc cagcaggtga tcgatggcct cctgcaccag cgcctcggac
      481 tcggtgtcga gggaggaggt ggcttcatcg aagatcagga tcggcggatt ctggagaatg
      541 gcccgcgcga tggccaggcg ctgccgctgg ccgcccgaca ggagctgccc gctttcaccg
      601 atcggcgcgt cgtagccgag cggcatcgcc gcgatgaagt cgtgggcgtt cgcggcgcgc
      661 gcggcggcct cgacttcacc tcgtgatgcg tccggccgtc cgtaggcgat gttggcggcc
      721 accgtgtcat cgaacaggat ggtttcctgc gtcacgatcc ccatgagccg gcgcaggtct
      781 ttcaggttga ggagccgggt gtcccggcca tccacctcga tcaccccctc ggtcggctcg
      841 taaaaacggg gaatcaggtc gaccagcgtg gtcttgccgg cgcccgacgg acccacgagc
      901 gccacggtct ctccctgccg ggcttcgaat gcgatgtctt tcaacaccgg cttcgcgccg
      961 tcgtaactga aaccgacttc ccggaaccgg accgagccgg tgaaacggtc gatgcgggcg
     1021 ccgccgtcgt cgagcggctc cgacggaaga tccatcagct cgaagacgcg ctcggcggcc
     1081 acgcggccgc gctggatctt gccgagggcc ccggacactt ccttgagcgg gtgcatcagc
     1141 atgaacatgg cgatgaggaa tgccgagaac tccccgccgg tgaggccaga tgatgggtcg
     1201 agcacctgta gtccgccata ccagaggacg acgaccccga ccgacacggc ccagaactcg
     1261 gagaggggtg cggccaggag gtcgatcgag agaagcctcg tcaccgttcg acggtaacgg
     1321 tcggtggcct tccggaaacg gtcgcgctcg gtgtcttcct gcgcgaaggc cttcacgacg
     1381 cgcaccgagt gcacggtctc ctggaggtgc tgctgcaggt cggcgaggcg ctgctgggcc
     1441 cgaccggagt gcttcttcag cttcacggcc agccgcccga gcaggaacat cagcggtgga
     1501 aggatcacga aggtggccat ggccagtcgc gcgtccgcct tgaaggtgat cgcaaggaag
     1561 acgatcacga gaaggccgtt gtgcagcacc tgcgtgaacc cggaaaccgt gaagctcttg
     1621 atcgactcga cgtcgttgat gacgcggctg atcaggttgc ccgagtggtt ccggtcgaag
     1681 gccgagaggg ggaggtggat cacccgcgcg taaagatcgt tgcggatggc ctcggtggcc
     1741 cgttgctcga ggcgcgtgaa gaggatcttc cggagatagt cgaacgtgtt cctgagcagg
     1801 atgaggatca acgccgcgat gcagacgaac tggaggacct ggcggcgcgg cgcgctggcc
     1861 aggatggcgt tgacgcccga ggtgagggat tgccgggcgc catccaggct catggtgcgc
     1921 aggtgaccga acgtttcgcc gaggacgacg ccgatcccag gcgggggcac cgcagcgggg
     1981 ggtgttccga gtggcacggc atgagcggca tcggccgcga agagaccgtc cacgatcggc
     2041 ttgatcatcc cgatcgaaaa cccggagagg agggcgtatc ccgccatgca gacgagcccg
     2101 agggcgaggg tccgccattc gggcttcagg taggcgagca gacgtcggtt gggattcatg
     2161 cggggaagga tgggggagga ccaagcggcc ctccccctcc gaaactacag gctaattgcc
     2221 gccccgcgcg gcgtcaacgg acgaggacca tcttccggct ctcgctgccg tccttcgaga
     2281 ccaggcggta gtagtagacg ccggagccga ccggcactcc ctcctggtcg ttgccgtccc
     2341 agttgacctc gtactcgccg accggcatgg gtcggtcgac cagcaggcgg accaggcggc
     2401 ccgtgggcga gtacacctcg atgcggaccg ggaccagctc attccccctg gaggcggact
     2461 ggatgcgcca gccgatcgtg gtgttcgggt tgaacgggtt cggtcggttc tgcgcgagca
     2521 ggaacggctt gaggcccgtt cccgcgtccg gggtctcggg aacgcccgtc tggtaataga
     2581 ggggttcacc gagccgcccg gtatgccgcg ggtcgtgatg gaacatcggc cagggactgg
     2641 ccagcttgtt gaacgtccag ggggtgtccc actggtacac cttctggtcg tacgactcgg
     2701 ccacgatctc ggtgagtccg tcgccgtcca ggtcccacgc ggccgggcca ccctcgatca
     2761 ggttgccggt ctggatgggg aagccgggta ggagcgtgcc gttggcatag aacccgtgga
     2821 gacggccgat ctggtcgccg atgatgattt ccggcacgcc gttgttgtcg atgtccacca
     2881 ggttggggct cgagcgcacc ggcacgttgg cgttcagcgc atcgcggatg aagaccggcc
     2941 atccggcgat cagcgtgcca ttgttccgcc acgccaccac gcggccgttc gaagcgccta
     3001 ccaccacgtc cggtatcccg tcgccggtga tgtccccgag cgccggcgag gaatcgagat
     3061 cctcgttgag ctggatcccc tgcggccagc cggctgccgc ggtgccgtcc ccgttcaggg
     3121 catacacgcg cgagtcgctg gcgccgaaga cgatttcgag ctgaccgtcg ttgtcgatgt
     3181 cggccacggc gggcgagctg gtgatttcgc cgccggctcc cgaattgccg ttggtggtga
     3241 aggggaagcc ggggagttcc tgccagttga ccgggttgaa gacatgcagc ttcttgtcgc
     3301 gcatcccgac gatcacctcg ggacggccgt cgccgttcat gtcggccagg gccggagtgc
     3361 catagttgta cgccgtgttg atcaccttga tgacgccgac ggtcgacggg ttggcgtcgc
     3421 catccacgat ttccgtgccg tcgtggtgga agccgtaaag caggcggccg cacatgacaa
     3481 agagctcgag atcgccgttg ttgtcgatgt cgcccatcgt cacggacgag agaggattcg
     3541 ggtcgccgag cgcctgggcg ttgatgttct tcggccagcc cggctccacc tcgccgaatt
     3601 cgttccagag atacatgctg gccagccacg agacggcggc cacctccggc gagccgtcct
     3661 tgtcgacatc gcccaccgcg ggggcgttcc agaagccggg cttccccgtg ttggtaagca
     3721 cgcccagggt gcggatgtcg ttgtccccgt cgaacagctc ggtgccgtcg ggcttcagtg
     3781 cgtacacctc gctggcgccc gccaggatct cgagatcgcc gtcgtagtcg aggtcggcca
     3841 gcgtgacgga ggcgttggtc gcggcctcga gctccaccgg gaactcgtcg tgcagcggca
     3901 tcgtcgtcga ggccgagaag gcgatggaga ggggcccttc gttgcccgag gagtcctggg
     3961 ctgccacctt gtaatagaag accgtcagcg gcgggagccc ttcgtcgttg tagtaggcgg
     4021 tccgcatcgt ggtgttctgg ttgatgcggc tgaaggggcc gaccgcggcg gaactgcggt
     4081 agacgttgta gccgtacagg tcggcgctgg tcaccggagt gaacgtcacc gcgatcgaag
     4141 tttcgaagcc gcgggccgtg agtccaccca gggttcccac gggcgagcgg gcatcgatga
     4201 gccagctggc cagcacggtg gacgggctgt aatggtcata gatactcaac cgaagcttct
     4261 tgttgccggc cgcgaagccc gtggtgtcgg tcatctggaa ccggaaggtg tcggaaggac
     4321 ggctgatcgc cagcgcgccg ccggccagcg tgccgaaggc caccgtggaa tcgttcacca
     4381 cgatgctggg gtccgtgctg cggagtaccg cgttcacgac ccgcgcctgt ccgagaccgg
     4441 cgttacggag ctcgacgcgc agggtgacat cctcgttcgg agcgaagacg ctgttgccgt
     4501 tgccgttccc cgtcgtgtcg cgcgggaact ggcggaacca ctggaagacg ggcgcgtgaa
     4561 tgtagagcac cacctcttcc acgatgctgg tggcgccggc ctgccaggtg acctggatct
     4621 tcggctccgt gcggtcgggg gccgtgcggc tgatggagag caccaaagga tcgcccgggt
     4681 tgacctgcgc gcccgctccg gccgccgcgt aggacgacgt gccgtcaatc accgtcacgt
     4741 agggtgacag cgatatcacg gtggccgtga tcggcgagga ggcgccgccc cggttcttca
     4801 ccggcagcga gagctcgatc gtttcgcccg cgtcgatctt ctggtcgccg ttgccggtgc
     4861 tcgatcccga gccgtcatcg tcgatgagct ggtccgccgc gtacaggtac ggagcgaccg
     4921 gcgtcgtcac cgtgatattg gcctggtatg gaacatggtt gtcggccacg acggtaagct
     4981 gggtcgtgcc ggtcgagtcg ggcacgaagg ggagcatgat ctggccgctc ccgttggtga
     5041 gcccgaccgc gtattcatcg ttgaccttgt tcaggcagac gcgggcgttc tggagcgggt
     5101 tgccgcccga gttcaccgtc accgtgaagg tgccggcgcc cagcgcgtgg gtggcggcat
     5161 gcgtcaccgc caggtcggcg ggcggctgcg tgaagagcgg catcgagggg tcgccgaagt
     5221 agatctgggt gtactgcgtc cagcgatgcg tgttgtcctg ctgcgccagc ggcacgaagg
     5281 gtagcttgca ggccgaggcg gcatgcccca gttcatgaat gccgtcctcg aagacaagct
     5341 ggtagtactc cgtctggtac gaccagcctg tcgaggggaa gtcgaaccgc gtcgacccga
     5401 ccgaggccac cgagccgcca tgcgagttga gcatgtaacg ttcggcgatg cagttgaagt
     5461 cgatggcgct cgacgtgcaa ttgatggcgt agaggaagaa cgtctcgttg ccgttgttgc
     5521 aggcgtcggc gtcggcgttg gtcagcgcct ggtcggcctt gccgaccgac atcgtgttga
     5581 tgtagccgtg tcccaggtga tggacgaagc cgaaaccggc gttcagcgag tcgagcaccg
     5641 tcggaatctt ctgctcgaga atgcaggtgg gccagctggc attcgggcag ttctcgtaga
     5701 gcttgaccac ccggtagttg cccggcaggt ggctgatccc ttcctcggcg atcgaggcgc
     5761 catcgaagag gatcgtgtcc ccgggattcc aggtctgagg aaagagcacc tcggccagcg
     5821 ccaggtagtc atcctggtaa ccgttccgga gcggggtcac ctcgtaggcc aggatcttgt
     5881 tcacgagtgt ggtggcctgg gccagcgtga ccgtcggcag gcgcccgatc cacacttcgg
     5941 ggtagagatc caccgagtcg accgggtcgg gcgggatgga accttcgccg aactcggcgt
     6001 cgccgtcggc gttccaggtg ccgtcgacgc actggaagta gaggtcggtc gggatttcct
     6061 cgccgctgaa gaattgcgtc ttgccgtagc gcgtcgggat cacgtcggtg tcgccgccga
     6121 ggagcaccca caccgtgccc cacttctgcg cggcgtcgcg gatgaagttg cggatcgtct
     6181 cctgcgtgtc cacgccctgg ggatagttgg ccttgatcca gttcacggtg cgcaccacgc
     6241 agggcgtgcc cgcgcgcgtt ttccagtccg ccagccgctg gtactgcgac tgctgtgtct
     6301 cgttcgtgat gatcaccatc tcgaccggcg tgccgtttac cgacgggcgg aaggtcgggg
     6361 caaagggggc cccgctgttc agtagcggag agatctcggc gccgaccgta cgaccggcca
     6421 cgtcaggcga gcggttcacc acgagatcct cgacctctcg atcgaagatg gcgtcggccc
     6481 acggctcgcg gcgcagcggc aggaaatcgt ccttgcgatc gcgcgtttcg ggttcgagcg
     6541 tgagcaccag ctcggcgcgc gtcacgatct cgaggcggcc gtccgattcg cgccagcgga
     6601 aggggcggta caccacaccg ccgagcgcgc gaccgcgcat gcggccggag gcgccgggtt
     6661 cggccagggt ggccggatag aatccttcgc cgtcggtcgg ccgggtcgtg gcagaaaagg
     6721 aaagggggag gaattcatcg ttgctgttgc gggtccggat cgtggccggg tggaactcgc
     6781 cccgcaacgc ctgcgtctcg aggacacgca cgtccaccga agcgatgcgc atctcttccg
     6841 gcaggcgaaa ggtgagcggc ttgaccggca ggtccggcat cccttcctcg gtgccgatgg
     6901 ctccgcccgg aagcgtgatg aagtcgacgt tcgagttccg ccggatgagg acgtcggtgg
     6961 cggacacctc caccgtctgc accaggcgga cgggctcggc ggcgccgccc acacccacca
     7021 tcgccagaag ggtcagggcg gaaaggagga agggtcgggt catcagggtc tcggaaaatc
     7081 gggagaggaa ggagaacaca ggacggccgg gggagagatt cattcgggga gcacctctca
     7141 acgggtcacg atgccggtcc acaggggacg ttgagccgac gggcgcggag ggttggctcg
     7201 acccaccagt tgcaccaaat aggctgggat cggggagatt atcgcagagg cagaagtcac
     7261 gggtcaacaa aggcttgccc ttccttgtgg tatgtatgga gatacggccg ggcactggac
     7321 acggattggg cgatggctac aattcgccac ccgaaagtcg gttcctgaag cgttcgaacc
     7381 gcccggtgtg agaaaataga cccaagaaca agatagtcac gacgaaagga gaatgcatgg
     7441 cttccgaaag aaccagggaa atcaagcgcc gccgcaagcg ccgcgaggag cggatcaagt
     7501 cccggcgcaa ggaatcgatt gccaaaggga agaaaggcgc cacccgaccg gcgcgctgag
     7561 gcccgatcgc ccgacgccgg tcaggccggc gcgggcgcgt ccggcctgtc acgaaccgag
     7621 gagtaccagg aggaaccggt aaccgccgat gcctgccagc cgacctgacg gaccccgcct
     7681 cttcacgttg gaggaggcca acgcgctcat cccgcgcctg gaaaccgccc tgtccgaaat
     7741 ggcggccttc cgggccgagc tcaagctgct ccggaaggac atcgaggtcc tgtcgttgat
     7801 cgccgcctcg gccggcggca acgagaaccc ggatgccgcg gagctcaaga acaagcgccg
     7861 ccgctaccgc tccgtcgcct ccgaaatcga gcggatcggc actggcctcg aggaggccgg
     7921 ctgcctcgtc aagcacccgg acgagggact cgtcgacttc ttccaccttc gcggagaccg
     7981 cctggtcttt ctctgctgga aggtggggga aaagcgtgtc gagcactggc accccctgag
     8041 cggggggtac gccacgcgga aaagccttgg ccaacccgag tcctgaccgc gcctcccaca
     8101 ccgtgccgcg gaacgcgccg gtcgccggct tgacagttca acgcccggcc gcctagcgtc
     8161 gaaccggccc ctcccaccgg caacacccgg atcgattccg ttccggcgcc tgcgcccgac
     8221 tccccgtccg gacaggagtg ccacctcatg ccgattgccg atctttaccc cgcacccccc
     8281 gcaaacctga ctccgttcga cgacgaggag gaagacgaag ccgtcgagct cccactcgcg
     8341 gcgccgccgc cacatgcgcc cgttcccggt gccgccgcgc tacggagccg gtgggaccgc
     8401 ggaccgcgcg aacgactgct ccgcgaagga ctgggcgccc tcaacgacgc cgaactgctg
     8461 gccctgatgt tcggcaccgg ctcgcgcggg gcaccggccg tgcaactggc cgagcggctc
     8521 ctggtggccc gtggtctcac cggccttgcg gcgatcgatc ccgtcgatct ctgcgtggaa
     8581 cgggggatcg ggccggcccg ttcggcccag atcgcggcct gtttcgagct gggaagacgc
     8641 ctctttggcc gcgacgaagg cgccgtcctc atcaaggggc ccgaggatgt gtacgaagcc
     8701 accaccgacc tgcgcggcgc ccgaaaggag cacttcgtca ccttccacct gaacgcgcgc
     8761 aaccaggtga tcgcccggga gacggtgtcg atcggcagcc tcaacgcctc gatcgtccac
     8821 ccgcgcgagg tgtacgaacc ggccgtccgc aattcggcgg cctcgatcat cctcgtccac
     8881 aaccacccga gcggcgagac cgatccctcc gacgacgacc tggctctcac gcgccggctc
     8941 gtgcaggtcg gtgaccttct cggcatcgcc atcgtcgatc acatcatcgt cgggcggcgc
     9001 ggcttcacca gtttcaagca gcagggttac atctgaggat ccggggggag gagcgcggag
     9061 gcgcccacgg cgccggcgcg ctcctccttc cgatcgaggg ccgctccctg taggctcccg
     9121 tccgtgacga cgctgaccgc cgaacaggta tcccatcacc tccggaacgt ccgggatcgc
     9181 ccgatcctcg tcctgggcga cgtgatgctc gaccgctatc tctggggcaa tgtccaccgc
     9241 atctccccgg aggcccccgt cccgatcgtg gatgtcgagt cggaaaccgt gcggttgggg
     9301 ggcgcggcca acgttgctca caacgtatcg gcgctgggcg cccgtcccat cctcgtggcg
     9361 gtggccggcg aggatcatcc cgcctcgctc ctccgggccg agctcgctgc gcgggggctt
     9421 tccgccgccg gcctcgtggt cgatgccggc cggccgacgt cgatcaagac gcggatcgtg
     9481 gctcgccacc agcaggtggt gcgcgccgac caggagagcc gcgaggagat caggggtccg
     9541 gtcgaggagc gggtcctggc cgtcgtg
//
LOCUS       DASVOD010000673         3335 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_878705, whole genome shotgun sequence.
ACCESSION   DASVOD010000673 DASVOD010000000
VERSION     DASVOD010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878705"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1160
                     /locus_tag="VF720_14415"
     CDS             <1..1160
                     /locus_tag="VF720_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880605.1"
                     /translation="SAIVWNVATGQPVSTFSEHRGTVGALAFHPDSRSILSAGSEGRV
                     MVWDAITGARTAEYHGHGSDVSAVAIAPTGDWSVTGEWDGTIRAWAWGVDDVRVIDGR
                     GTDLAPVRYLSLDVDPSGRLAVATGQYDNVEVRELHEGTKVVEVWPNRAPTAVFSDTS
                     GFVVGDDTGRLRRYLLSGDVADDIPAYNGPVVALAASPGTGRVASLGKGGRIRLWGVP
                     GLVPLREWSAPDPDVRAIAFDPTGSRMASGGTDGLVRIWSPESGALLDSLACPASTVS
                     QLAFQPGGPRVAAVLAGGAVILFDPARSEPDTLLANGDAGLSLAFSPDGGRLAVGSHD
                     QLIYLFDVARRAPILRLHGHIARVSVVNFLPDRHTLLSASHDGTIRLWNSN"
     gene            complement(1209..2279)
                     /locus_tag="VF720_14420"
     CDS             complement(1209..2279)
                     /locus_tag="VF720_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880606.1"
                     /translation="MSIDSPPASRLAFPTRRLGMPPLALLEPAPRAGRSRVDLMWHEG
                     RLGLRSRTPVDGSDWSFAPPTLAFLHHLEPPLPAMIDAYQASLPPHGTAQVRLRTSPE
                     GRIGAWIDMSREAMARLLVERDWLLGRVGAGWLVEMGQRGEGVLAGRDGPLLAPATPA
                     TWLPGRDADGGVIPLECHVASFTQPGPAINAGLLASLGTLLDRAGIGVCRWAEWGSGY
                     GNLTAWLATRLGSDGCAVELDGRSRQLLVRNAVRHFPTVRLEDPEPTRRRSMQAPASS
                     DHDFDILVIDPPRPGFSSRLDGLADSPHRPAWVLSLHCDARGLDADGVALARLGFAPD
                     AWIGVDAFPGTPYLEVITLWKR"
     gene            2384..3310
                     /locus_tag="VF720_14425"
     CDS             2384..3310
                     /locus_tag="VF720_14425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880607.1"
                     /translation="MLRILAILVGLVLASDAAGKVLNVEFKFTPFTGDPDAADMVQTV
                     PGHAVVWMNNIPVAEQEIEEEEVPVLFDEREVAASIWVPTESLGGMVRQGRNTIRIEF
                     QPSNGRKPYRAQLRWSTVLDETSQREQGGTHAATNEADAGLDDRPATGKVTFEREFTA
                     EFAMDQPWHHAPAVTALTDDDRKALAELVVRRLKAFAPDFAGIYELIGANGGIDVEAV
                     RKEKCLDKAWEAGIRIAAVPTDRIEFLTTGNAEVVIRAKEGPLYVPENMAVFETITDP
                     EMQMCIGSALSGVYPPRLIVVRAKDGSWNVAY"
BASE COUNT          514 a         1091 c         1226 g          504 t
ORIGIN      
        1 actccgccat cgtctggaac gtggccaccg ggcagcccgt ctccaccttt tccgaacatc
       61 gaggcacggt cggcgccctt gcgttccacc cggacagccg cagcatcctg tcggccggat
      121 ccgagggtcg cgtgatggtc tgggatgcga tcaccggcgc ccggaccgcc gaataccatg
      181 gccacgggtc cgatgtgtcg gccgtggcga tcgcgccgac gggggactgg agcgtgaccg
      241 gcgagtggga cggcacgatc cgcgcctggg cgtggggagt cgacgatgtg cgggtgatcg
      301 acggacgcgg caccgacctg gcccccgtcc ggtacctgtc gctggatgtc gatccgtcgg
      361 gccggctcgc ggttgcgacc ggccagtacg ataatgtcga ggtccgggag ctgcacgaag
      421 ggacgaaggt cgtggaggtg tggccgaacc gagcgccgac cgccgtcttt tccgatacct
      481 cgggcttcgt cgtgggtgac gacacggggc ggctgcggcg gtatctcctt tccggggatg
      541 tggcggacga tatcccggcg tataacggtc cggtcgtggc actggccgcg tcaccgggca
      601 ccgggagggt ggcatcgctc ggcaaggggg ggcggatccg actgtggggg gtgcccggtc
      661 tcgtgccgct ccgggagtgg tcggcgcccg accccgatgt ccgggcgatc gcgttcgacc
      721 cgaccgggag ccgcatggcc tcgggtggca cggatggcct cgtccggatc tggagtccgg
      781 aaagcggggc tctgctcgac agccttgcct gtcccgcttc gaccgtgagc cagttggcgt
      841 tccagccggg tggcccccgc gttgcggcgg tgctggccgg tggggcggtg atcctgttcg
      901 acccggcgcg atcggagccc gatacccttc tcgccaacgg cgacgcggga ttgtcgctgg
      961 cgttcagccc ggacggggga cggctggctg tcggctcgca tgaccagttg atctacctct
     1021 tcgacgtggc ccggcgggcg cccatcctcc ggcttcacgg gcacatcgcc cgcgtgagcg
     1081 tggtcaactt cctgccggac cgtcacaccc tcctgtcggc cagccatgac ggtacgatcc
     1141 ggctctggaa cagcaattga cctcggcggg gcgccccgat tgggcggtgg ccggcacgcg
     1201 ttcggccatc agcgcttcca gagcgtgatc acctccaggt agggcgttcc ggggaacgca
     1261 tccacgccga tccacgcgtc gggcgcgaag cccaggcggg ccagggcgac gccatcggcg
     1321 tcgaggccgc gcgcgtcgca gtggagtgac aggacccagg cggggcgatg gggcgagtcg
     1381 gcgagcccgt cgaggcgcga tgaaaatccg gggcggggtg gatcgatgac gaggatgtcg
     1441 aaatcgtggt cgcttgatgc gggcgcctgc atgctgcggc ggcgcgtcgg ctccgggtct
     1501 tcgagccgca cggtcgggaa gtggcggacg gcgttgcgga ccaggagttg ccgactcctt
     1561 ccatccagct cgacggcgca accgtcggac ccgagacgcg tggccagcca ggcggtgagg
     1621 ttgccgtagc ccgatcccca ctcggcccag cggcacaccc cgatgcccgc acgatcgagc
     1681 agcgtaccga gtgaggcaag gagccccgcg ttgatggcgg ggcccggctg cgtgaaggag
     1741 gccacgtggc attcgagcgg gatgacgccg ccatccgcat cacgccccgg aagccaggtg
     1801 gccggtgtgg cgggcgcgag gagcgggccg tcccttccgg cgaggacccc ctcgccccgc
     1861 tgtcccatct ccacgagcca tcccgcgccg acccggccaa ggagccaatc gcgctcgacg
     1921 agcagccggg ccatcgcctc gcgcgacatg tcgatccacg cgccgatgcg tccttcgggt
     1981 gatgtccgca ggcgcacctg cgccgtgccg tgcggtggca ggctggcctg gtaggcgtcg
     2041 atcatcgccg ggagtggcgg ctcgagatga tgcaggaagg cgagtgtcgg cggcgcgaac
     2101 gaccagtcgg atccgtcgac cggcgtccgg ctccgcaggc cgaggcgccc ctcgtgccac
     2161 atgaggtcca cccggctgcg gccggcccgg ggggccggtt cgaggagcgc gagaggaggc
     2221 atcccgaggc gtcgggtggg aaatgccaga cgcgacgccg gcggagaatc gatcgacacg
     2281 gggtacctct cggagtgcgg accgtggcac gttgccggcc cgggcgttag gttaccatcc
     2341 gtgcgtcgac gatcgtctcg aacgcgtgtc cccggagatc cgaatgttgc gaatccttgc
     2401 gatcctggtc gggcttgtcc tggcatccga tgccgcgggc aaggtcctga acgtggagtt
     2461 caagttcacc ccattcaccg gtgatcccga tgccgccgac atggtgcaga cggttcccgg
     2521 ccatgccgtc gtatggatga acaacatccc ggtggccgag caggagatcg aggaggaaga
     2581 agtgccggtg ctcttcgacg agcgcgaggt ggccgcctcg atctgggtgc ccacggaatc
     2641 gctcggcggc atggtgcgcc agggcaggaa caccatccgg atcgagttcc agccctcgaa
     2701 cggcaggaag ccgtatcgcg cccagcttcg ctggtcgacc gtgctggatg agacgagcca
     2761 gcgggagcag gggggcacgc acgcggccac caacgaagcc gacgccggcc tcgacgatcg
     2821 cccggccacg ggaaaggtga ctttcgagcg ggagttcacc gccgagttcg cgatggacca
     2881 gccatggcac cacgccccgg cggtgaccgc gctcacggac gacgaccgga aggcgctggc
     2941 cgagctcgtc gtcaggcgcc tgaaggcctt tgcgcccgac ttcgcgggga tctatgagct
     3001 gatcggcgcc aacggcggca tcgacgtgga ggccgtccgc aaggagaagt gcctggacaa
     3061 ggcatgggaa gccggcatcc ggatcgcggc cgtcccgacc gacaggatcg aattcctgac
     3121 caccgggaac gccgaggtgg tgatacgcgc gaaggagggc ccattgtacg tgccggaaaa
     3181 catggccgtg ttcgaaacga tcaccgaccc ggagatgcag atgtgcatcg gctcggccct
     3241 ttccggcgtc tacccacccc gactgatcgt ggtccgggcg aaggacggtt cctggaacgt
     3301 ggcctactag gcgatcggca cggcatgtcg ggtcg
//
LOCUS       DASVOD010000674         2763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_878724, whole genome shotgun sequence.
ACCESSION   DASVOD010000674 DASVOD010000000
VERSION     DASVOD010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878724"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..558
                     /locus_tag="VF720_14430"
     CDS             115..558
                     /locus_tag="VF720_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7880608.1"
                     /translation="MKISRRGLYALRALLVLAENAPGEVVTIAAVSAGQAIPQKFLES
                     ILVTLKNARLVESVRGAQGGYRLKRAAADITLGEVVRLMDGPLAPLGDAAELRHLAEE
                     DKAHPGLFRVLLDVRDAAAKILDGTTLADLATRNRKLVRQRTTRG"
     gene            630..1238
                     /gene="rsmD"
                     /locus_tag="VF720_14435"
     CDS             630..1238
                     /gene="rsmD"
                     /locus_tag="VF720_14435"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX7880609.1"
                     /translation="MGVLRVVAGRWRGRHLSAGPARGQTAIRPTADRVRTSLFDRLMP
                     ILSGARVLDLCAGTGALGIEALSRGAAHVTFVDRSGAAIRLIEGNLESLGLKGSGEAS
                     VRRDEAVHAVAALARQGEKFDLVLADPPYAGEMASSIVLAMDDHPILAEDGWFLLEHD
                     RRITLPKALRKIEPFDQRQYGDTCLTFYRESRDEDSPLSGDL"
     gene            1207..1731
                     /gene="coaD"
                     /locus_tag="VF720_14440"
     CDS             1207..1731
                     /gene="coaD"
                     /locus_tag="VF720_14440"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX7880610.1"
                     /translation="MKTALFPGTFDPITEGHLDIIRRGLPIFERVIVAVARGVHKETL
                     FSLEERKELVEISVAAAIGAGPDGTPGNVSVVEFDGLLVDLADRLEVDVIIRGLRFVS
                     DFEYEFQLALMNRQLNRRVETVFLMPSARYSFLNASIVREVARLGGDISSLVPPAVAE
                     RLTKRFQSGRGQGS"
     gene            1735..>2763
                     /locus_tag="VF720_14445"
     CDS             1735..>2763
                     /locus_tag="VF720_14445"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEX7880611.1"
                     /translation="MPIRLSPRLDNVRPSLTLSLDDRVRSLQAAGKNIINLTVGQPDF
                     DTPAWIGDAARAAITRGATRYTSPIGTLPLRQAAAAWFRAKTGLDYTAEEILVSSGSK
                     HAIYNALQALLVPGDKVIVPSPFWSSYPDLVHMAGGVPVHPAGTLESGWKLTPALLDA
                     AITPKTRVLLINSPNNPTGALYHRGEAEALARVILERDIAVISDKIYDRLVYDNLTPV
                     SFAAVLPGMRARTIAINGVSKAYAMTGWRIGFAAGPPDIIEGMGRYQAQATSNPSSIS
                     QEAALAALTGTGPELETMLAAYQRRRDAVVAALSSLPGVELVPPDGAFYVFFRVRQLL
                     GRSIGGVRF"
BASE COUNT          428 a          961 c          935 g          439 t
ORIGIN      
        1 cgtgaccggg gatgccacac cggatcgtgt ggccgagcgg ctggcggaac gactggtcct
       61 gcgtgacgcc gacgtcattg ccaccctcgg ggcaagccgg aagaagggca cccgatgaag
      121 atctcccgcc gcggcctcta cgcccttcgc gcactcctcg tcctcgccga gaacgcgccg
      181 ggcgaggtcg tcacgatcgc ggcggtctcg gccgggcagg ccattcccca gaaattcctg
      241 gagtcgatcc tcgtcacgct gaagaatgcc cgcctcgtcg agagcgtgcg cggcgcgcag
      301 gggggatacc ggctgaagcg cgcggcggcc gatatcacgc ttggcgaggt ggttcgcctg
      361 atggacggcc ccctggcacc gctgggagat gccgccgagt tgcgccacct ggccgaagag
      421 gacaaggcgc accccggcct cttccgggtg ctgctcgacg tgcgcgacgc ggcggcgaaa
      481 atcctcgatg ggacgacgct ggccgacctc gcgacgcgca accgaaagct cgtccgccag
      541 cggaccactc gcggttgacc ctcgcaccat cgatctgata accttattaa atacagcagt
      601 ttgcagtatt cgcttcaagg agtttgtgga tgggtgtgct ccgcgtcgtg gcgggtcgct
      661 ggcgggggag gcatctttcc gccggacctg cgcggggaca gacggccatc cgtcccacgg
      721 cggatcgggt gcggacgtcg ctcttcgatc gactgatgcc gatcctgtcc ggagcccggg
      781 tgctcgacct ctgtgcgggg acgggggccc tcggcatcga ggccttgagc cggggggcgg
      841 cccatgtcac cttcgtcgac cgttcgggag cggccatccg gctgatcgag ggaaatctcg
      901 aatcgctcgg gctcaagggg tcgggtgagg cgagcgtgag gcgcgacgaa gcggttcacg
      961 ccgtggcggc gctggcgcgg cagggagaaa aattcgatct cgtcctggcg gatccgccgt
     1021 acgcggggga gatggcatcc tccatcgtcc tggccatgga cgatcacccg atcctggcgg
     1081 aggacggctg gttcctgctc gaacatgacc gacgcatcac gctgccgaag gcgctccgga
     1141 agatcgaacc cttcgaccag cgccagtacg gcgatacgtg cctgaccttc taccgggagt
     1201 cgcgcgatga agacagccct ctttccgggg acctttgatc cgatcaccga ggggcacctc
     1261 gacatcatcc ggcggggcct gccgatcttc gagcgggtga tcgtggcggt ggcgcgcggc
     1321 gtccacaagg aaacgctctt ctcgctggag gagcggaagg aactggtcga gatctcggtg
     1381 gccgcggcca tcggcgccgg gccggacggc acgccgggga acgtgagcgt ggtggagttc
     1441 gacggcctcc tcgtcgacct ggccgaccgg ctcgaggtgg atgtcatcat ccgcgggctc
     1501 cgcttcgtct ccgacttcga gtacgaattc cagctggcac tgatgaaccg ccagctcaac
     1561 cgccgggtgg agacggtctt cctgatgccg tcggcccgct actcgttcct gaacgccagc
     1621 atcgtccgcg aagtggcccg cctcggcggc gacatctcct cgctcgtccc tccggcggtg
     1681 gccgagcgcc tcacgaaacg gttccagtcc ggtcgcggcc agggaagctg acgcatgccg
     1741 atccggcttt caccgcgcct cgacaacgta aggccgagcc tcaccctctc gctcgacgac
     1801 cgcgtccgct cgctccaggc ggccggcaag aacatcatca acctcacggt cggccaaccg
     1861 gatttcgaca cgccggcctg gatcggcgac gcggcccggg cggccatcac gcgcggcgcc
     1921 acccgttaca cctcgccgat cggaacgctg ccgctccggc aggcggcggc ggcctggttc
     1981 cgggcgaaga ccggcctcga ctacaccgcc gaggagatcc tcgtctcgtc ggggtcgaag
     2041 catgccatct acaacgcgct gcaggcgctc ctcgtgcccg gggacaaagt catcgtgccg
     2101 tccccgttct ggtcgagcta ccccgacctc gtgcacatgg ccggcggcgt gccggtgcat
     2161 ccggccggca cgctcgagag cggctggaag ctcacgccgg ccctcctcga tgcggccatc
     2221 acgccgaaga cgcgcgtcct cctcatcaac tcgccgaaca acccgaccgg cgctctctac
     2281 caccgcgggg aagccgaggc gctggcccgc gtgatcctcg agcgcgacat cgccgtcatc
     2341 agcgacaaga tctacgaccg gctggtgtac gacaacctga cgcccgtttc gttcgccgcc
     2401 gtcctcccgg ggatgcgcgc gcggaccatc gccatcaacg gcgtctcgaa ggcctacgcg
     2461 atgacgggtt ggcggatcgg cttcgcggcc ggcccccccg acatcatcga ggggatgggg
     2521 cgctaccagg cgcaggccac cagcaacccg tccagcatct cgcaggaggc ggccctggct
     2581 gcgctgaccg ggacagggcc cgaactcgag acgatgctcg cggcctacca gcgccggcgc
     2641 gacgcggtgg tggccgcgtt gtcgagcctt cccggtgtgg agctggtgcc gcccgacggc
     2701 gctttctacg tcttcttccg ggtgcggcaa ctcctcgggc gctcgatcgg cggggtgcgg
     2761 ttc
//
LOCUS       DASVOD010000675         5041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_669043, whole genome shotgun sequence.
ACCESSION   DASVOD010000675 DASVOD010000000
VERSION     DASVOD010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669043"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="VF720_14450"
     CDS             <1..588
                     /locus_tag="VF720_14450"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880612.1"
                     /translation="VDPSEDEVGLDTSIAIDGNGNVHISYHDASIGNLLWARRTVSGW
                     SVMTVDDGFDATGLYSSIAVDSQGHPHIAYQNGTTANLDYAYIPLSVGVGDGPRSTPI
                     ARLRAVPNPIPGDRVRLYVDGPSAVGVEGVVIVDVTGRRIRELPLPDTTSGSATVDWD
                     VRDEQGVRVSPGVYYFRTAGSSAVVRSGGRLVVTR"
     gene            complement(578..1948)
                     /locus_tag="VF720_14455"
     CDS             complement(578..1948)
                     /locus_tag="VF720_14455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX7880613.1"
                     /translation="MLSSLKVRIVFWYSLVFILTLLAVQLITAQAIRRLLHDELDASL
                     RAETDWILHSVANARLYRLTDAQVRAELTSRSINNSRKEFIEIYDEKGNLFYRSPNVV
                     AGGVLRPARSASDRALTLPAFGGRDVRLFSRADQGFQVTVAYPLTDIHAAMDKLRMAF
                     IVLMPAALLLMAGGGIFLVSRFLRPIKGVSSYAEKLITQPLDEELPQLPGPPADEIAV
                     LREQVHLAAERMRGSMRQVLSFSTLASHELRTPLTVMRQELEDALDADRTPEELRKAA
                     ASFYDETLRLSTIVDDLLSLGTMQAGTFRLNAESTPLLAMLRDFNEEARPLVRDQGVV
                     IFLSGDPDLVARLDTSRMRQVLFNLLDNVVKHGGGNCRISLSCFRHDSSAVLCFGDDG
                     RGIPEDGRARVFDPFYRGRGPSGNGTGLGLALVRGIVEAHGGRITLASAVGRGTTFRI
                     ELPASA"
     gene            complement(1952..2680)
                     /locus_tag="VF720_14460"
     CDS             complement(1952..2680)
                     /locus_tag="VF720_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX7880614.1"
                     /translation="MAIAEARSPGRSRTEPEPGPHILIVEDEPKISRSLQAQLARHGY
                     VVDPAYDGSEGARKAIQGQHDLILLDINLPDGSGLDILRELRSRCRTTPVLVVSARDT
                     VGDRVDALQLGADDYLVKPFDSAELLARIQAIHRRAGSQQTLVLTAGDLTMDLVKRQV
                     RRGNVQIALTAREFALLEFFLRNQRQVLTRKRIAEQVWGYKFDTGTNVVGVYVSYLRK
                     AVDDGFTPRLIHTVLGEGFMLKDE"
     gene            complement(3037..3927)
                     /locus_tag="VF720_14465"
     CDS             complement(3037..3927)
                     /locus_tag="VF720_14465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX7880615.1"
                     /translation="MERKTAHDFDQELLVLFDAYVHGVIDRRTFLDRANKFAVGGVTA
                     LMLLEQLSPKFAEAQVVKPDDSRIKVEWLEYESPKGSGKMRGYLVKPAKSEAKLPGIL
                     IIHENRGLNPHIEDVTRRMALENFVAFAPDALFPLGGYPGNEDKARELFQKLDAEKTR
                     QDFIAAAALLRARPEVSGKVGGVGFCWGGGMVNYLATKPGSLDAGVPFYGSAPNTADV
                     PAIKIPMMIQSAETDDRINASWPDYETALKAAKVTYERHLYPGTQHGFNNDTTPRYDA
                     KAAKLAWDRTVAFFKANLRK"
     gene            complement(4048..4572)
                     /locus_tag="VF720_14470"
     CDS             complement(4048..4572)
                     /locus_tag="VF720_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880616.1"
                     /translation="MTDPEPAVPAPQRNMLEEFLDSWDKNNIIVLNLLKALPPGALGI
                     KPMDGSLTLGQHFSHLHSVRLDLVKENAPDVVTDATADEWADERDPARHTAQLEESSR
                     VVREAVASRVASGRAMNLHYDSPLLLIQHLLWHEAYHYGQIKLALKLAGQALPDKEMG
                     RVSWGVWMRRTKQP"
     gene            complement(4574..4936)
                     /locus_tag="VF720_14475"
     CDS             complement(4574..4936)
                     /locus_tag="VF720_14475"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7880617.1"
                     /translation="MTITRIIPMLPVASIARSIEFYRKLGFETEKRNDEWGWAMLALG
                     DCRLMLDQSIQRHPSGPRESILYLYPDDIEAYHREVRRNGLDIPDLDITFYGLTEFRL
                     DDPDGNRLWIGQDRRKGS"
BASE COUNT          846 a         1601 c         1704 g          890 t
ORIGIN      
        1 gtggatccgt ccgaggatga agtcggactc gatacctcca tcgcaatcga cggcaacggg
       61 aacgtccaca tcagttacca cgatgccagc atcggcaacc tcctctgggc gcgccgcacg
      121 gtctccggct ggagcgtgat gaccgtcgac gacggcttcg acgccacggg cctttactcc
      181 tcgatcgccg tcgacagcca gggacatccc cacatcgcct accagaacgg gacgaccgcc
      241 aacctcgatt acgcctacat cccgctcagc gtgggggtcg gggatggccc gcgctccacg
      301 cccatcgccc ggctccgggc ggttcccaac cccattccgg gggaccgcgt gcggctctac
      361 gtggacggac cctcggccgt gggcgtggag ggcgtggtca tcgtcgacgt caccgggcgc
      421 cggatccgcg aactgcccct tccggacacg acctcgggct ccgcgaccgt ggactgggat
      481 gtccgcgacg agcagggtgt ccgggtgagc cccggtgtct actacttccg gaccgcgggg
      541 tcatcggccg tcgtgcgatc cggcggccgc ctggtcgtca cgcgctagcc ggaagctcga
      601 tccggaaggt cgttccgcgg cccacggcgc tggccagggt gatgcggccg ccgtgggcct
      661 cgacgatccc ccggaccagg gccaggccaa gcccggtgcc gttaccggag gggccgcgac
      721 cgcggtagaa agggtcgaag acccgggcgc gtccgtcttc gggaatgccg cgaccgtcat
      781 cgccgaagca gaggacggcg ctgctgtcgt gtcggaagca cgacaggctg atgcggcagt
      841 ttccaccacc gtgcttgacg acgttatcga ggagattgaa caggacctgg cgcatccggg
      901 acgtgtcgag ccgggcaacc aggtcgggat cgccgctcag gaagatcacc actccctggt
      961 cccgcacgag cggacgagcc tcctcgttga aatcgcgcag catggccagg aggggagtcg
     1021 attcggcgtt gaggcgaaac gttcccgcct gcatcgtccc caggctcagg aggtcgtcga
     1081 cgatggtgct gagccgcagc gtctcgtcgt agaacgacgc ggcggccttg cgcagctcct
     1141 cgggggttcg atcggcatcg agcgcgtcct ccagctcctg gcgcatcacg gtcaaggggg
     1201 tcctcagctc gtgggacgcc agggtggaaa acgacaggac ctgccgcatg ctgccgcgca
     1261 tccgctcggc ggcgaggtgc acctgctcgc ggagaacggc gatctcgtcc gccggaggcc
     1321 ccggaagctg cggcagttcc tcgtcgagcg gttgggtgat cagcttttcg gcatagctgc
     1381 tcaccccctt gatcggcctc aggaagcgtg acacgagaaa gatgccgcca ccggccatga
     1441 ggaggagggc cgccggcatg agcacgatga aagccatccg gagcttgtcc atggcggcgt
     1501 ggatatcagt gagcggatac gccacggtca cctggaaacc ctggtccgcc cggctgaaga
     1561 ggcgcacatc ccgcccgccg aaggccggca aggtcagtgc ccggtccgag gccgaacggg
     1621 cggggcggag aacgcccccg gcaacgacgt tcggggaacg gtagaagagg ttccccttct
     1681 cgtcgtagat ctcgatgaat tccttccggg agttgttgat gctgcgcgag gtcaactccg
     1741 cgcggacctg ggcgtccgtg aggcggtaca ggcgggcatt ggcgaccgag tgaaggatcc
     1801 agtccgtctc ggcccggagc gacgcgtcca gctcgtcgtg cagcagcctc cggatggcct
     1861 gcgcagtgat cagctgcacg gccaggagcg tcaggatgaa gaccagcgag taccagaaga
     1921 cgatccggac tttcagggag gaaagcacgc gtcactcatc cttgagcatg aatccctcac
     1981 cgaggacggt atggatcagc ctgggggtga acccatcgtc caccgccttg cgcaggtagc
     2041 tgacgtatac cccgacgaca ttggtccccg tatcgaactt gtaaccccac acctgctcgg
     2101 caatccgctt ccgggtcagg acctgtcgct ggttgcgcag gaagaactcg agcagcgcga
     2161 actcccgggc cgtgagcgcg atctggacgt ttccgcgccg gacctgcctt ttcacgaggt
     2221 ccatcgtgag gtcgccggcg gtgaggacaa gggtctgctg gctgccggcc cggcgatgga
     2281 tggcctggat gcgggccagg agctcggcgg agtcgaacgg cttcacgagg tagtcgtcgg
     2341 ccccgagttg cagggcgtcg acccggtccc ccaccgtgtc gcgggcggaa acgaccagga
     2401 ccggggtggt ccggcaccgg ctccggagct cccgcaggat gtcgaggccc gatccgtccg
     2461 gcaggttgat gtcgaggaga atcaggtcgt gctgcccctg gatggccttc cgggcccctt
     2521 ccgatccgtc gtacgccgga tcgacgacgt aaccgtgccg ggcgagctga gcctggagcg
     2581 agcgcgagat cttcggctcg tcctcgacga tcaggatgtg cggccccggt tccggctccg
     2641 tgcgggagcg acccgggctc cgggcttcgg cgattgccat ctccgtccct tcgtcccgcg
     2701 gaggcatgga ccggccacgg gtatggagca tcccgacacc ggcaaacggt gaccggctgc
     2761 aggaacttac aaaccgtcgt gtgggagtgc ctctggtgcc ccggggaggg tgcggcctcg
     2821 tttgggcacc gtcggcgagc agttgccaaa accggcaaac cgctcctgcg gacaactact
     2881 ggaccttgta tataaagaga gtagcgtgaa acggcaaggc cttatgccaa ttgattcaag
     2941 aattcaccgc agggcacccg gaaattacgc gaatcgcgga gtcggcgcgg actcgatccg
     3001 tggcctcaca cgggaatgga cgcgccgctc gcgcggctac ttccgaagat tcgccttgaa
     3061 gaacgccacc gtccggtccc aggccagctt ggcggccttc gcgtcatagc gcggcgtggt
     3121 gtcgttgttg aatccgtgct gtgtgcccgg atagaggtgc cgttcgtacg tcaccttcgc
     3181 ggccttcagc gccgtttcat agtcaggcca gctggcgttg atccggtcgt ccgtctcggc
     3241 cgactggatc atcatgggga tcttgatggc aggcacgtcg gccgtgttcg gcgcggagcc
     3301 gtagaacgga acgccggcat ccaggctccc cggcttcgtg gcgaggtaat tcaccatgcc
     3361 gccaccccag cagaagccga cgccgccgac cttgccgctg acctcggggc gtgcccggag
     3421 cagggcggcc gcggcgatga agtcctggcg cgtcttctcg gcatcgagtt tctggaagag
     3481 ctcgcgcgcc ttgtcctcgt tgccgggata gccgcccagc gggaagaggg catcgggcgc
     3541 gaaagccacg aagttctcca gcgccatgcg ccgcgtgaca tcctcgatgt gcgggttgag
     3601 gccacgattc tcgtggatga tcaggatgcc gggcagtttg gcctccgact ttgccggctt
     3661 gaccaggtag ccgcgcatct tgccggaacc tttgggcgat tcgtattcca gccactccac
     3721 cttgatgcgg gaatcgtccg gcttcacgac ctgggcctcg gcgaacttcg ggctcagctg
     3781 ttcgaggagc atgagggccg tcacaccgcc gacggcgaac ttgttggccc ggtcgaggaa
     3841 ggtgcggcga tcgatgacgc cgtgaacgta ggcatcgaag aggacgagaa gctcctggtc
     3901 gaaatcgtgg gcggtcttgc gttccatgga aggctcctgc ggtagcgtca gggtggcgcg
     3961 gcgttgcgat gtcatgaggc tccgattcgc gcggccatgc acggaccggt ccagcccatg
     4021 tccggagtgg ggtggtcgtg aggaatctta cggctgtttc gtcctgcgca tccagacgcc
     4081 ccagctgacc cgccccatct ccttgtcggg aagcgcctgc ccggccaatt tcagggccag
     4141 cttgatctga ccgtagtgat aggcctcgtg ccagaggagg tgctggatca ggaggagggg
     4201 tgaatcatag tgaaggttca tggcgcggcc ggaggccact cgcgatgcca ccgcctcacg
     4261 gacgacgcgg gaactctcct cgagctgcgc cgtgtggcgc gcggggtcgc gctcgtccgc
     4321 ccattcatcg gcagtggcat ccgtcaccac gtccggggcg ttttccttga ccaggtcgag
     4381 ccgaaccgag tggagatgcg agaagtgctg cccgagcgtg agactcccgt ccatcggctt
     4441 gatgccgagc gcgccgggcg ggagggcctt gaggagattc aggacgatga tgttgttctt
     4501 gtcccaggaa tcgaggaact cctcgagcat gtttcgctgc ggcgccggca cggcgggttc
     4561 cggatccgtc atttcagctc ccctttctcc tgtcctgccc gatccagagc cggtttccat
     4621 ccggatcgtc gagccggaac tccgtgaggc cgtagaacgt gatgtcgagg tccggaatgt
     4681 cgaggccgtt ccgccggact tcccgatgat aggcctcgat gtcatccgga tacaggtaca
     4741 ggatcgactc gcgcgggccc gatggatggc gctggatgga ttggtccagc atgagacgac
     4801 agtctcccag ggcaagcatg gcccaacccc actcgtcgtt ccgtttttcc gtttcgaaac
     4861 cgagcttccg gtagaactcg atgctccgcg cgatgctcgc aaccggaagc atcgggatga
     4921 tgcgggtgat ggtcattcgc gtcgtgtccg ttgacatccg tggcctcttt gggtgcggtc
     4981 ttatcacggc ccgaatcgtg gccgcgacat gttacctgtt ccggaacagg ctctcggaga
     5041 t
//
LOCUS       DASVOD010000676         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_462543, whole genome shotgun sequence.
ACCESSION   DASVOD010000676 DASVOD010000000
VERSION     DASVOD010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462543"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VF720_14480"
     CDS             complement(<1..752)
                     /locus_tag="VF720_14480"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HEX7880618.1"
                     /translation="MSEPDFLNFVRVLDRWKRPLIALVLGTAIVSAAVSYVLPKWYMA
                     RAVILPPQDSEIGSALGSLLQGITLPGVGNVAPAGTETQLFVTILDSRTLRTKLVEQF
                     DLMRVYKARNIDEAIRSHRLLAHAAITDEGAVEILVEDRDPKRAADQANAWVDLLDDY
                     NKTSRMSSARKNRIFVEERLKETTEKLALAEDHLAAYQQEHKAVALTPDLSSAVEAGA
                     GLMAHRMQLLVQLNRYNDIYRSDTPQLIQTRA"
     gene            complement(739..>1765)
                     /locus_tag="VF720_14485"
     CDS             complement(739..>1765)
                     /locus_tag="VF720_14485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7880619.1"
                     /translation="ATIEAPPLLRFALSGIGFAVAVLALRLVDRKALLAGAGQDPTAA
                     DVRPSAVGSSAVIDAPALVAPPVVNLSVVILTCQSARTLDATMASVAFAGEIVVIDSG
                     STDETLTIARRHGARIASRSMADGWAAQRNFGQTRAAGRWILALDSDEILDETLAKAI
                     HRVVSQPFDATAPAGYTIRMLNFFLGRPLRYGGLDRDDHLRLVRNGVGRWVGAVHEKL
                     GVTGKVGHLPGLVHHMTGVTFEQRLAKAGVYARKRAELWRAARRRPSVVRAIWEPLRF
                     LAGRLVIRGGWRDGLPGFVWWWLQATELLGAHLILAVEPLYRGETAPKIAAASDSSFE
                     PETHVGT"
BASE COUNT          302 a          563 c          620 g          280 t
ORIGIN      
        1 tcggcccggg tctggatcaa ttgcggcgtg tcgctccggt aaatgtcgtt gtaccgattg
       61 agctgcacca ggagctgcat ccgatgcgcc atcagcccgg cgcccgcctc gacggcggaa
      121 ctcaggtccg gtgtcagcgc cactgccttg tgctcctgct ggtacgcggc caggtggtct
      181 tcggccagcg caagcttctc ggtagtctcc ttgagccgct cttccacgaa aatgcggttc
      241 ttgcgcgccg acgacattcg tgacgtcttg ttgtagtcgt cgagcaggtc gacccaggcg
      301 ttggcctggt cggcggcgcg cttcgggtcc cgatcctcga cgaggatttc aaccgctccc
      361 tcatccgtga tggcggcgtg cgcgagcagc cggtgacttc ggatcgcctc atcaatgttc
      421 ctggccttgt agacccgcat caggtcgaac tgctcgacca gtttggtacg cagggtgcgg
      481 ctgtcgagga tggtgacgaa cagctgggtc tcggtgccgg ccggggcgac attgccgacg
      541 cccggaagcg tgatcccttg aagcagggag cccaacgcgc tgccgatctc agagtcctgg
      601 ggcggcagga tgaccgcccg ggccatgtac cacttgggga ggacgtacga gacagcagcg
      661 gagacgatgg cggtacccag gacgagggcg atgagcggac gtttccagcg atcaagaacg
      721 cggacgaaat tgaggaaatc aggttccgac atgggtctcc ggttcgaacg aggaatcgga
      781 agcggccgcg atcttaggtg cggtttcccc gcggtacaag ggctccacgg cgaggatcag
      841 gtgggcgccc agcaactccg tagcctgcag ccaccaccag acgaagccgg ggaggccgtc
      901 gcgccacccg ccgcggatga cgaggcgccc ggccaggaag cgaagtggtt cccagatggc
      961 ccgcaccacg gaggggcgac ggcgggccgc acgccagagc tcggcgcgct tccgcgcgta
     1021 gacgccggcc ttcgccagtc gctgctcgaa cgtgacgccg gtcatatggt gaacgaggcc
     1081 gggcaggtgg ccgaccttgc ccgtcacgcc gagtttttcg tggacagccc ccacccaccg
     1141 accgacgccg ttccggacga gtcgaaggtg gtcatcgcga tcgagaccgc cgtagcgcag
     1201 agggcggccg aggaagaagt tgagcatgcg gatcgtgtaa ccggccgggg ccgtcgcatc
     1261 gaacggttgg gaaacgacac ggtggatggc cttcgccagc gtctcgtcga ggatctcgtc
     1321 gctgtccagg gccaggatcc agcggccggc agcgcgggtc tgtccgaagt tccgctgggc
     1381 cgcccaccca tccgccatgc tccgcgaggc gatgcgcgct ccgtgccggc gggcgatggt
     1441 caacgtctcg tcggtgctgc cggaatcgat caccacgatc tcgccggcga aagccacgct
     1501 ggccatcgtg gcatcgagag tcctggcgct ctggcaggtg aggatgacca cggaaaggtt
     1561 gacgacgggt ggggcgacga gtgccggcgc atcgatgacg gcggacgatc caacggcact
     1621 cggacgcaca tcggctgccg tggggtcctg gccggccccg gccagcagtg ccttgcggtc
     1681 caccaggcgc agggccagca cggcaaccgc gaatccgatc ccggacaagg cgaagcgaag
     1741 caggggcggc gcctcgatcg tggcc
//
LOCUS       DASVOD010000677         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_254063, whole genome shotgun sequence.
ACCESSION   DASVOD010000677 DASVOD010000000
VERSION     DASVOD010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254063"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VF720_14490"
     CDS             complement(<1..684)
                     /locus_tag="VF720_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX7880620.1"
                     /translation="MSREPQPNALLESAGNNPLDLSAEEYRRLGHRLVDQLADHLATI
                     RTSRTTDGKTPGELRAILGNDRMLPANGADAEALLARATQLVLDHSLYIGHPLFLGYV
                     VGSPSPLGALGDLLASTINPNVGGHSLAPIATEMERQTVRWIAELIGYDPACGGLLVS
                     GGNMANFVGFLAGRRAILGAGSRTAGVGDRKAVVYATDETHTWIDKATDLFGMGTDAV
                     RKVPRLAVPV"
     gene            839..>1728
                     /locus_tag="VF720_14495"
     CDS             839..>1728
                     /locus_tag="VF720_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880621.1"
                     /translation="MRNVHRPPATAFSALILGVMFLAATPRLARAVESNDGWWGDYDR
                     AGIEWGATAVADWHGDIVVSGEFASIDARPMPWAARWNGSEWIAMSNGLASELLEIAV
                     VGDELYGVVPYTWNASTPRLRHWTGIRWEIVPGQPLDLESLVVSPGPVLFARTGSQFH
                     RWDGTTWTTWGPPATFRRSGVPWDDGVAIALNTGQVVVIHQDSMSLLGSPTGGVAGLA
                     VLEGSLVAGYQEGRRFEPNGYLVEISRVRRWDGESWKPMGSSFDNLATHFLERDGGFE
                     LVCQVRDTGPAMIRRWNGTE"
BASE COUNT          274 a          529 c          629 g          296 t
ORIGIN      
        1 aaccggcacg gccaggcgcg gcaccttgcg cacggcgtcg gtgcccatgc cgaagagatc
       61 ggtggccttg tcgatccagg tgtgggtttc gtcggtggcg tacaccacgg cctttcgatc
      121 acccacgccg gccgtacgcg aaccggctcc cagaatcgcg cgccggccgg ccaggaagcc
      181 gacgaagttg gccatgttgc cgcccgacac gaggagcccg ccgcacgccg ggtcgtagcc
      241 gatgagctcg gcgatccagc gcaccgtctg ccgctccatc tccgtggcga tcggcgcgag
      301 cgagtgcccg ccgacgttcg ggttgatcgt ggaggccagc agatcgccca gcgcccccag
      361 cggcgacggc gacccgacca cgtagccgag aaagagcggg tgcccgatgt agagcgagtg
      421 atcgaggacg agctgcgtgg cgcgggcgag gagggcctcc gcgtcggcgc cgtttgcggg
      481 gagcatacgg tcgttgccga ggatggcgcg cagctcgccc ggcgtcttgc cgtcggtggt
      541 gcgggacgtc cggatcgtgg ccagatgatc ggcgagctgg tccaccaggc gatggccgag
      601 tcgacgatat tcctcggcgg aaagatcgag cgggttattc cccgcggact cgagaagggc
      661 gttcggctgg ggctctcggc tcatggcggc tccacgattg aacggtatga ggtacggccg
      721 caaggatagc gcatcccggt agcggcgctt gctccagggc attgcaattt catgggttat
      781 tggacccgaa cgcgccaatt tgatggggga attgcagggt gcggccgatg aatggggcat
      841 gagaaacgta catcggcctc cagccaccgc cttctccgcc ctgatcctcg gcgtgatgtt
      901 cctggctgca actcctcgcc tcgcgcgggc agtcgagagc aatgacgggt ggtggggcga
      961 ttacgaccgc gcgggaatcg aatggggcgc cacggccgtg gccgactggc acggtgacat
     1021 cgtcgtcagc ggcgagttcg cctcgatcga cgcgcgcccc atgccgtggg cggcgcgatg
     1081 gaacgggagc gaatggatcg cgatgtcgaa cgggctggcg tcggagttgc tcgagatcgc
     1141 ggtcgtgggg gatgagcttt acggtgtggt gccgtacacg tggaatgcgt cgacaccgcg
     1201 cctgcgtcac tggacgggca ttcgatggga aattgttccc ggccagcctc tcgaccttga
     1261 gtcgctcgtc gtgagtccag gtccagtcct gttcgcgcgg accggctcgc agtttcatcg
     1321 ctgggacggg acgacttgga cgacctgggg tccgcccgcc accttccgcc gatccggcgt
     1381 gccgtgggac gacggggtag cgatcgccct caacacgggg caggtcgttg tcatccacca
     1441 ggactcgatg tcgctcctcg gttcgccgac aggtggcgtg gccggcctgg cggttctcga
     1501 ggggtcgctg gtggccggtt accaggaggg acgtcggttc gagcccaacg gatacctcgt
     1561 ggagatcagt cgagtgcgac gttgggacgg cgaatcctgg aaaccgatgg gcagttcctt
     1621 cgacaacctg gccacgcatt tcctggagcg ggatggcggg ttcgaactcg tctgccaggt
     1681 cagggacacg ggccccgcga tgatccgtcg ctggaacggc accgagtg
//
LOCUS       DASVOD010000678         3628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_151157, whole genome shotgun sequence.
ACCESSION   DASVOD010000678 DASVOD010000000
VERSION     DASVOD010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151157"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..>3628)
                     /locus_tag="VF720_14500"
     CDS             complement(144..>3628)
                     /locus_tag="VF720_14500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880622.1"
                     /translation="DNNVVYGRVMADNVLYFPGDSKRLIAIDHQPGLENGDYIEYEIQ
                     VTSNSFPLEVTLCWTDYPASPASSIQLVNDLNLTVSNGATVYKGNVYSAGASIAGGSF
                     DNRNVEEACLIGNPALGTWTVRVEGFAVPVGPQPFGLVVTGVVDGGAGALYLDKAEYG
                     STGTVGVKVIDTNATSPLTVHITSPSEPGGEDVTLTGANGVLTGTLKLAPWSPGAPHG
                     AGHLSSLGRDALGTLGVDVADDTLRVAHGDQLTATYLDANPVATLTARAFVAIEQPTI
                     TSVSADSRGSSAAVINWTTNLNSDSRVYYGLTPALELGSLFQADQVLAHQILIPGLTT
                     SATYYYDVESVGPNGNLVRDNNGGDHYRVTIDPPADILLVIGDEASFDRLEAWTEAAA
                     AAGWSLDIWSGTLADNAQLGNTTGGLRSYKAVIWQPGFSQYPPFSDTQRTTIGNYLNG
                     GGRLLAVGHDIAWAFGDVGSPVYSIARRDWLRDVLHTTWQEDPATWSSNIGYASDPIS
                     GAYTGGVPYTPLGSTQAGDEVDVFLGNGTSNYVWRSNATTPDDIGFRWLSNVNNGSPD
                     SAVWGGAPSRLATMYFEYTAITPPFGTPTATRTDILDKTLIWLIGRDQPAIAVTAPTV
                     ASTVTTDSVDVTWSEVISGASAANRKIEYSADGGDSWILVANGVGPSPYRWGLNALPN
                     RTTMRVRVTVTDNGTPALTGSGAMSGNFSLQRPSADAEGPLVVPGSPVASPNPYTAGM
                     AATLAARLTDAARGGLNVTAAEWSYGPAAATAGSGTAMTGVFTTPTVDVSASIPTRTI
                     NPGPTQFWVRGRDSAGNWGAATALQVQVNPGMFAQAEVLCAADTALCPGDTLNLSLQV
                     VNVGPGADTLHVELVDQGPGPGLTLVGGPVVITTSVAAGDTLVVPYTVVTASNAADAA
                     STVLKARAWPTDFPSDADSCLTLITVMAPVVGASCPSDTLVISPDTVPLAFSIANTGS
                     KPDSIRWTITDLGPGPDIVMQGGPSISGVSYLGGNGPTSDNLPAVTVEAFVAAAPGQT
                     ATLQLVTWPDSDPSLADTCDVTVGISMASAVPEGGIPLVFAAGPAHPNPFTATARISF
                     TLPATGHAAIRIHSADGRLVRTLVDGTMEAGPHLVDWDGRDGDGTAVPSGVYFYRLTG
                     DGGQVAGGRVLRVR"
BASE COUNT          531 a         1131 c         1386 g          580 t
ORIGIN      
        1 gctgtccgca ggcccgcacg acgcgacctg ggacgggcgg cgcgaggacg gcacaccggt
       61 ggccgacggt gtctacttct accggctcga cgccctgggc gagagccgca cgatccgttt
      121 cgcgcaggtg cgttgagcgg cgcctagcgc acccgcagca cccgtccccc ggccacctgt
      181 ccgccatctc ccgtcaggcg atagaagtag acgcccgatg gaacggcggt gccgtccccg
      241 tcgcgtccgt cccagtcgac caggtgcggg ccggcctcca tcgtgccgtc cacgagcgtg
      301 cgaacgagac gtccgtcggc cgagtggatg cgaatggcgg cgtggccggt ggccggcagc
      361 gtgaagctga tccgggccgt ggcggtgaac gggttgggat gggccggtcc ggccgcgaag
      421 acgagcggga tgcctccttc gggaacggcc gaggccatgg agatgccgac tgtcacatcg
      481 caggtatcgg cgagtgacgg gtcggagtcg ggccaggtga cgagctggag cgtggccgtc
      541 tggccgggcg ccgccgcgac aaatgcctcg acggtgaccg ccgggaggtt gtcgctggtg
      601 ggtccgttgc cgcccagata cgacacgccg ctgatcgacg ggcccccctg catcacgatg
      661 tccgggccgg ggcccaggtc ggtgatcgtc caccggatcg aatcgggctt gctgccggtg
      721 ttggcgatgc tgaaggcgag tggcacggtg tcgggcgaga tgaccagcgt gtccgacgga
      781 cagctcgcgc cgaccacggg cgccatcacc gtgatgaggg tgaggcacga gtcggcgtcg
      841 gacgggaagt cggtgggcca ggcgcgcgcc ttgagcaccg tactggctgc atcggcggca
      901 ttcgaggcgg ttaccaccgt gtacggcacc acgagggtgt cgccggccgc gacgctggtg
      961 gtgatgacca ccggtccgcc gaccagcgtg aggccgggac ccggtccctg gtcgacgagc
     1021 tcgacatgca gcgtgtcggc gccgggcccg acattgacga cttggagcga caggttgagc
     1081 gtgtcgccgg ggcagagagc ggtgtcggcg gcacaaagga cctcggcctg cgcgaacatc
     1141 cccggattca cctggacctg gagggcggtg gccgcgcccc agttgccggc ggaatccctg
     1201 ccgcggaccc agaactgggt ggggccggga ttgatcgtgc gcgtggggat cgacgcgctc
     1261 acgtcgacgg tgggagtggt gaagacgccg gtcatcgcgg tgcccgatcc ggcggtcgcg
     1321 gccgcggggc cgtagctcca ttcggcggct gtcacgttga gacccccccg agccgcgtcg
     1381 gtgaggcgtg cggcaagcgt ggcggccatg ccggccgtgt acgggttggg tgacgcaacc
     1441 gggctccccg gcacgaccag cggcccttcc gcatcggcgg acgggcgctg gagcgagaag
     1501 ttgccgctca tggcgcccga tccggtcagt gccggtgtgc cgttgtcggt aaccgtgacg
     1561 cgcacgcgca tcgtcgtgcg gttcggcaac gcgttcaggc cccaacggta gggggagggc
     1621 cccacgccgt tggccaccag gatccacgag tcgccgccgt cggccgagta ttcgatcttc
     1681 cggttggcgg ccgacgcgcc cgaaatgact tcggaccagg tgacatcgac cgagtcggtc
     1741 gtcacggtcg aggccaccgt cggcgcggtg acggcaatcg ccggctggtc gcggccgatc
     1801 agccagatga gggtcttgtc gaggatgtcc gtgcgggtgg cggtgggggt gccgaacggc
     1861 ggcgtgatgg ccgtgtactc gaagtacatg gtggcaagcc gcgagggcgc acccccccag
     1921 accgccgagt cggggctgcc gttgttgacg ttggagagcc agcggaagcc gatatcgtcg
     1981 ggcgttgtgg cattgctgcg ccagacatag ttcgaggttc cgttgccgag gaagacatcc
     2041 acctcgtcgc cggcctgggt cgagcccaga ggcgtgtagg gcacgccgcc ggtgtaggcg
     2101 ccggaaatcg gatcggacgc gtagccgatg ttgctgctcc atgtggccgg gtcttcctgc
     2161 cacgtggtgt ggaggacgtc gcgcagccag tcgcgccggg cgatgctgta gacaggcgaa
     2221 ccgacgtcgc cgaaggccca ggcgatgtcg tgccccaccg cgaggaggcg tccgccgccg
     2281 ttcaggtagt tgccgatcgt ggtgcgctgg gtgtcgctga agggggggta ctggctgaag
     2341 ccgggctgcc agatcaccgc cttgtaggag cgcaggcccc cggtggtgtt gccgagctgc
     2401 gcgttgtcgg ccagcgtccc cgaccagatg tcgagcgacc agccggccgc ggccgcggct
     2461 tcggtccagg cttccagtcg gtcgaaggag gcttcgtcgc cgatgacgag gaggatgtcg
     2521 gccggcgggt cgatggtgac cctgtagtgg tcgccgccgt tgttgtcccg gaccaggttg
     2581 ccgttcgggc cgaccgactc gacgtcgtaa tagtaggtgg cgctggtggt gaggccgggg
     2641 atcaatatct ggtgcgcgag cacctggtcg gcctggaaga ggctgcccag ctcgagggcc
     2701 ggcgtgaggc cgtagtacac ccgcgaatcg gaattgagat tggtggtcca gttgatgacc
     2761 gccgccgaac tgccgcgcga gtcggccgac acgctggtga tggtcggctg ctcgatggcc
     2821 acgaaagccc tggcggtgag cgtggccacc ggattggcat cgaggtacgt ggcggtgagc
     2881 tggtcgccgt gggccacgcg gagcgtgtcg tcggccacgt ccactcccag cgttcccagg
     2941 gcgtcacgtc ccaggctcga gaggtggccg gcgccgtgcg gcgcgccggg cgaccagggg
     3001 gccagcttga gcgtgccggt caggacgccg ttggcgccgg tgagcgtgac gtcctcgcca
     3061 cccggctcac tgggggaggt gatgtgcacg gtgagcggtg acgtggcgtt cgtgtcgatg
     3121 accttgacgc cgacggttcc ggtcgaaccg tactcggcct tgtcgagata gagcgcgcct
     3181 gcgcccccgt cgaccacacc ggtgacgacc agcccgaacg gctgcgggcc gaccggcacg
     3241 gcgaaccctt ccacccggac cgtccaggtg ccaagggcgg gatttccgat gaggcaggct
     3301 tcctccacgt tgcggttgtc gaagctgccg ccggcgatcg aggcgccggc cgagtagacg
     3361 ttccccttgt agacggtggc gccgttcgac acggtcaggt tcaggtcatt gacgagctgg
     3421 atggaggagg ccggcgaggc cgggtagtcg gtccagcaga gcgtgacttc cagcgggaac
     3481 gagttcgagg tcacctggat ctcgtactcg atgtaatcgc cgttctcgag gccgggctgg
     3541 tggtcgatgg cgatcagtcg cttcgaatcg cccgggaagt agaggacgtt gtcggccatc
     3601 acgcggccgt aaacgacgtt gttgtcgg
//
LOCUS       DASVOD010000679         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_879160, whole genome shotgun sequence.
ACCESSION   DASVOD010000679 DASVOD010000000
VERSION     DASVOD010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879160"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF720_14505"
     CDS             <1..362
                     /locus_tag="VF720_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7880623.1"
                     /translation="IRYQKLIRTIPVSDYVVDYAVRLARATRPGRSGSPDIDAVSKYV
                     SWGAGPRASQYLVLGAKTLAALRGNLTPSVDDVKRIARPVLRHRILTNFNAEAEGIRT
                     ETIIDRLLEAVPEGNRK"
     gene            389..1291
                     /locus_tag="VF720_14510"
     CDS             389..1291
                     /locus_tag="VF720_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7880624.1"
                     /translation="MAPNDTLRFLDPKVVGELSSLDLVAKLVVEGFLTGLHKSPYHGF
                     SVEFAEHRQYMPGDPIRHVDWKLYAKTDRYFVKMYEEETNLRAHLLIDTSASMGYASP
                     GQVTKLRYGTFLGAALAYMMINQQDSVGLLTYADTVRKFIPPRAVKTHLKVLYAEMDA
                     LKPSATTDTLKSLQDLADRLHRRGLVILISDLMDDPRGVLTALKHFRHRRHEVIVFHV
                     LDPHEIEFPFRDESGFIDMETGREVLTQPWEIAKDYRRNFQAWQEAYARECRANLIDY
                     VPIDTSTPFDRTLLAYLEKRRRLA"
     gene            1224..2471
                     /locus_tag="VF720_14515"
     CDS             1224..2471
                     /locus_tag="VF720_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880625.1"
                     /translation="MTRPRRLTGRCWRTSKNGGDWPEAEGATMVNHSGFVAGCFRSAP
                     ILQLVARLLLVVLSCGVLAACGEDDSSTDSVAIGGTDGRIYDSEYVARRFFWLEDLRE
                     PSQLPLDIQIALFKTTRDRADDNRNGFETFVVRGSLQGDDVGIVVSDEVRRLVLNGDY
                     TVTDIRGVPGRVIALRERLLDNERLYASYFFGGGSTPESHVGTYWTSRSPEDDLRVQV
                     VALQESDLGDNALPDEFIKPTLRLERRNIYDLQDNRINPEALRITIRAIDTADRSSKI
                     DGLELLTAMGLDAYNNETHLSCFGGHATTSTRGPDGLVDDVWIDTRNGYLVFPDQRPF
                     DPELSDIFDTSIGLVLNECRTRVIGWELVNPTSSLPGDESYRRVSMDKQDRERNPALY
                     NRLRAKPDTRRYEITGTFELRPD"
     gene            2503..3270
                     /locus_tag="VF720_14520"
     CDS             2503..3270
                     /locus_tag="VF720_14520"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX7880626.1"
                     /translation="MTRIGIVAHSAEGASLCFITACREGQKLLGPHMHPEIVMSAIPM
                     ALSMPGWESDDHAVVGAVLRRGVEQVAAAGADFFVCPDNTAHIVLETIAETLPIPGLH
                     IADVVAGEMKARGWRTAGLVGTRWTMNGPVYPRALGALGLAALVPDERRQGEINRAIF
                     EELCQGVFAPATTAMFVEAIQELAKRGAECAILGCTEIPLIIDAGNSPLPVLDSTRLL
                     AIYAVREAISGRLSTDRPGWLNCGCFDEEGLPPARPQ"
BASE COUNT          621 a         1108 c         1055 g          575 t
ORIGIN      
        1 tcatccgcta ccagaagctg atccgcacga tccccgtttc ggattacgtg gtggattacg
       61 ccgtgcgcct cgcgcgcgcc acccgccccg gacggagcgg cagccccgac atcgacgccg
      121 tgagcaagta cgtgtcgtgg ggcgccggcc cgcgcgccag ccagtacctc gtgctcggcg
      181 ccaagacgct ggccgcgctc cgcggcaacc tcacgccgtc ggtggatgac gtgaagcgga
      241 tcgcccggcc ggtgctgcgc caccgcatcc tcaccaactt caacgccgag gccgagggaa
      301 tccgcaccga aacgatcatc gaccgcctgc tcgaggccgt acccgaggga aaccggaagt
      361 agcggcccgc cgcgctcccg ggtcgtagat ggcaccgaac gacacgctcc gcttcctcga
      421 cccgaaggtg gtcggcgagc tgtcgagcct cgacctggtg gccaagctcg tggtggaagg
      481 gttcctcacc ggcctccaca agagccccta tcacggcttc tcggtggaat tcgccgagca
      541 ccgccagtac atgccgggcg atccgatccg ccacgtggac tggaagctct acgccaagac
      601 cgaccgttat ttcgtgaaga tgtacgagga ggaaaccaac ctccgcgcgc acctgttgat
      661 cgacacctcg gcctcgatgg gctacgcgag ccccggccag gtgaccaagc tgcggtacgg
      721 caccttcctc ggggccgcgc tggcctacat gatgatcaac cagcaggact cggtaggcct
      781 cctcacgtac gccgacaccg tccgcaagtt catcccgccc cgtgcggtga agacgcacct
      841 caaggtgctg tacgccgaga tggacgcgct caagccctcg gccaccaccg acacgctcaa
      901 gtcactgcaa gacctggccg accgcctgca tcgtcgcggc ctcgtgattt tgatctccga
      961 cctcatggac gaccccaggg gcgtgctgac ggcactcaag catttccgcc atcgccgcca
     1021 cgaggtcatc gtgttccatg tgctcgaccc ccacgaaatc gaattcccct tccgtgacga
     1081 atcgggcttc atcgacatgg agaccgggcg cgaagtcctg acccagccct gggagatcgc
     1141 caaggactat cgcaggaact tccaggcctg gcaggaggcg tatgcccgtg aatgtcgcgc
     1201 gaacctcatt gactacgtgc cgattgacac gtccacgccg tttgaccgga cgttgctggc
     1261 gtacctcgaa aaacggcggc gactggcctg aggcggaagg ggcgactatg gttaaccaca
     1321 gtgggttcgt cgccggctgt ttccgttcgg ccccaatcct gcaactcgtc gcccggctac
     1381 tcctggtcgt cctgtcttgc ggagtcctcg ccgcttgcgg cgaggacgat tcgtccaccg
     1441 attccgtcgc catcggcggc accgacgggc ggatctacga cagtgagtac gttgcccggc
     1501 gattcttctg gctggaggat ctgcgcgagc cgagccagtt gcccctggac atccaaatcg
     1561 cgctgttcaa gaccacccgt gaccgtgccg acgataaccg gaatggcttt gaaacttttg
     1621 ttgtccgagg gtcgctgcag ggggacgacg tcggcatcgt cgtgagcgac gaggttcgtc
     1681 ggctcgtctt gaacggggat tacacagtta cagatattcg gggcgtgccc ggccgcgtca
     1741 tcgcactgcg cgaacggctt ctcgacaatg aacgcctgta cgccagctat tttttcggtg
     1801 gcgggtccac gccggaatcc catgtgggca cctactggac atccaggtcg ccggaagatg
     1861 acctgcgggt acaggtcgta gcccttcagg aatccgacct cggtgacaac gcgctcccgg
     1921 acgagttcat caagccgacg cttcgactcg aacggcgcaa catctacgat ctgcaggaca
     1981 acaggatcaa ccccgaggcg ctccgaatca caatccgcgc aatcgacacc gccgatcgca
     2041 gcagcaagat cgacggactg gagctcctga ccgccatggg cctcgacgca tacaacaatg
     2101 aaacgcactt gtcctgcttc ggcggccacg cgacaacgtc aacgcggggt ccggacggcc
     2161 tcgtggacga cgtgtggatc gacacgcgga acggatacct ggtcttcccc gaccagcgac
     2221 cgttcgatcc cgaactgtcc gacatcttcg acacatcgat cgggttggtc ctgaacgagt
     2281 gccggacccg tgtcatcggt tgggaactgg tgaatccgac gtcaagtctc ccgggcgacg
     2341 agtcctaccg ccgtgtgtcg atggacaagc aggatcgcga gcgcaatccg gccctttaca
     2401 acaggcttcg cgccaagccg gacacccgtc gctacgagat caccgggact tttgaactca
     2461 ggcctgactg acgggcggat ggaacgggct tctgcacaac ccatgacccg catcggcatc
     2521 gtggcccata gcgccgaagg cgcttcctta tgcttcatca ccgcctgccg cgaagggcag
     2581 aagctcctgg gcccccacat gcaccccgaa atagtcatga gcgccattcc gatggcgctg
     2641 agcatgccgg ggtgggagtc ggacgatcat gcggtggtcg gcgcggtgct caggcggggc
     2701 gtggaacagg tggccgccgc cggcgcggac ttcttcgtct gtcccgacaa cacggcgcat
     2761 atcgtgctgg agacgatcgc ggaaacgctg ccgattccgg gattgcatat cgcggatgtc
     2821 gtggcgggcg agatgaaggc gcgtggatgg cggacggccg ggctggtcgg cacgcggtgg
     2881 acgatgaacg gcccggtgta tccgcgggcg ctgggagcgc tgggcctcgc cgcgctggtg
     2941 cccgacgagc ggcggcaggg agagatcaac cgcgcgatct tcgaggagct ctgtcagggc
     3001 gtgttcgcgc ccgccacgac ggcgatgttc gtcgaggcca tccaggagct cgcgaagcgc
     3061 ggcgcggagt gcgccatcct cgggtgcacc gagattccgc tcatcatcga cgcggggaat
     3121 tcgccgctgc cggtgctcga ctccacgcgg ttactggcga tctacgcagt gcgcgaggcg
     3181 atttccggac gcttgtcgac ggaccgcccg ggatggctca actgtggctg tttcgatgaa
     3241 gaagggttgc cccccgcaag accgcagtaa ggtctgcggc ggaccacggg gtcatcattg
     3301 ctgagcccca aaaggcagga ggcaacccat atgccacggt acgtcggtct ggatgtgca
//
LOCUS       DASVOD010000680        13618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_463023, whole genome shotgun sequence.
ACCESSION   DASVOD010000680 DASVOD010000000
VERSION     DASVOD010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463023"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VF720_14525"
     CDS             complement(<1..1050)
                     /locus_tag="VF720_14525"
                     /inference="COORDINATES: protein motif:HMM:NF021632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEX7880627.1"
                     /translation="MSETVFPTAGRALSVRMLEEELNAVWKESVARVPGARPGSDTGP
                     ITRAALMTLVAVVDGKVAADRMTAMIAELTRQTPCRALIVELDGGGERATDHPGTAAE
                     PLSAFASAHCHATSRGRPVCSEQITLRSTPDGDPHLSNLVLPLLLPDLPVVLHWPGVA
                     SMMAPAGPPPHPLPRGAQFLCDLDPYVGQVILDSTLAGNPEAYLANVLALAERPAGPG
                     MRPIDLNWVRLLPWRDALADAVDRCAIDVATIREARIESHPDDAGVMPVRPRLLAGWL
                     RDRLRGTTGGAFRAWIDSAGPRGESAPGRIIRLILAMADGRELSIHVEQGTLAVSHGR
                     DSFRFSRPAEDDHLLC"
     gene            complement(1047..2588)
                     /gene="zwf"
                     /locus_tag="VF720_14530"
     CDS             complement(1047..2588)
                     /gene="zwf"
                     /locus_tag="VF720_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX7880628.1"
                     /translation="MPTLPNPLREGLPRERMPDPATLVIFGATGDLTRRKLVPAVFRL
                     FQRRHLPPGFLVMGVSRTELDDTAFRAEVTKAIAEHVGEDAVQSPEAREFATLFHYIG
                     GDFATKETWPQLGKRLAEIESAANRPSNRLFYLATPPAAFAPILEGLAQSQLVPREAR
                     QSPWARVVVEKPFGHDLESARALNRLISTIFDESQIYRIDHYLGKETVQNILVLRFGN
                     GIFEPLWNRRHVDHVQITVAEEVGVEQRAGYYEKAGVLRDMLQNHLMQLVSLVAMEPP
                     VPYRGEPIRDEKVKVIRALKPLAPADVATRVVRGQYAAGSIDGASVPAYRAEPGVNPE
                     SKTETWLAARLEIDNWRWGGVPFYVRSGKRMPRRATEIAIQFKAPPQQLFESVGADGL
                     SANVLALRIQPDEGIALRFGAKRPGPDIKIQPVKMDFLYGSSFGTDVPDAYDRLLLDA
                     LLGDPSLFIRRDEIEGAWAWVQPILDAWAADPSIPLHPYDAGHWGPPEADRWIQADGR
                     EWRHL"
     gene            2827..4062
                     /locus_tag="VF720_14535"
     CDS             2827..4062
                     /locus_tag="VF720_14535"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX7880629.1"
                     /translation="MIARWMGALGLSLLLASPALGQEADSVGTTSDELSGKLEGVIEQ
                     LQTLQSDTDKLKKIKVTGYVQARYESSQASDDSVRAAGNPLVLTPANLDRFYIRRARL
                     KVTYDPSERTQAVVYFDGGADRQIRLLEAYVQMSDPWTVDQKHILTIGQFNIPFGYEI
                     ERSSSVRELPERSRAENVLFPGERDRGVKLVGRWSDRLETVAGVLNGGGINQVDYPNT
                     DPTAAKDFVGRARLSFGKFDVAASASVGEHVIGLTGPDVKTDRTRFGTDAQGYWELPK
                     AGGGTLRGEVYVGEEFNPDSVAVLTTRPTNTAPVRFLKPGAEAAHLDSEFLGWYVMLV
                     QNLGDRFQAAARYDVYDRNTDVDGDDYRRWNVGLNWFYGGYTRFTLSYESPDVESTAG
                     APVPNPDDNLLTFQAQFKF"
     gene            4073..4933
                     /locus_tag="VF720_14540"
     CDS             4073..4933
                     /locus_tag="VF720_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7880630.1"
                     /translation="MTRHTILPARWRRIAGLALLGLSLVTCVASAAGKAITIKGSDTM
                     VILGQRWVERYMATHPGSVLQITGGGSGTGIAALINGNTDICQASRPMKTDEKKKLRD
                     RYQTMGVEIPVARDGLSIYVNEANPIQEFTMEQLRGIYTGQILNWKELGGADEPIVVY
                     SRESSSGTYVFFKDHVLGGADFAPQVQTLPGTAAIVNAVSKDKRGIGYGGVAYAKGIR
                     VPALKKDAAAPAILPTEAAVKDGSYALSRDLYFYTRKKPDGAVKEFIDWVLSAEGQKL
                     AIDVGYFPVN"
     gene            4998..5642
                     /locus_tag="VF720_14545"
     CDS             4998..5642
                     /locus_tag="VF720_14545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX7880631.1"
                     /translation="MPAFFSVVLATWNRAPRLSKAIDSVLAQTDDDWELIVVDDGSTD
                     DTAAVVESYRRNRRVVWVPRPHEGVWPAKNAGAAEARGRWITFLDSDDAYMPEHLAAR
                     RRCIVAGPEIRFWHGGVTVLGPPEMHSVPDADDPTRLIPLADCAIGASFVVKSTWWAR
                     LGGFDPTLWAADTDLLKRAAALESGAIARCAEPTYLYIRTPGDGVCEAERLRTR"
     gene            complement(5635..7068)
                     /locus_tag="VF720_14550"
     CDS             complement(5635..7068)
                     /locus_tag="VF720_14550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880632.1"
                     /translation="MTDIMSAVAPPLPPKATVGPAVMSLRPPSRPLQLITLFLAAMFL
                     TSCGDDDDPPDPEPVAMIRGQIERGGGYIGAPDRLAPEGLPSPTHLVGAVVTVERIDD
                     DGNFIDVTADTAVSDQNGRFVVSVNADGEADLVVAAVRDNERYKALVSRPVRADSTVV
                     VAPLNDETTGEAEIWAWLQDHPQLGSIPRPDVSLDVNSSVAAIVITGAELVASAGDAI
                     AGQVEAVDRFHLEEKVGTSAIGLAAARTARRAALVRLDEDLDTALDPSNENQARTIHA
                     HEAWFVREREAWTMNGIRPEVLAVAREAGMREYRRRAASARSDLKRVMSEAAALQRAR
                     TMAAAVDTLLEMAGVSPAGLSTVAMAGVTLRNSMYGIGSDAAILAEFNAYENTVVNVM
                     AAAFPGTAANEQLAHTQLVLPGGGAQDNLMIFLSGADTAEKVAGAYAEFYGLAEDQAF
                     GVLSNGNVPEASRRPFARALMLVVMPI"
     gene            complement(7070..8707)
                     /gene="murJ"
                     /locus_tag="VF720_14555"
     CDS             complement(7070..8707)
                     /gene="murJ"
                     /locus_tag="VF720_14555"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEX7880633.1"
                     /translation="MTEATRRQMGVATLIWMVAVALSRVMGLLREMVIARMAGADGHT
                     DVYFTAFNLPDIVNNLVAGGTLSVTFIPIFLGFMIRKEEERGWKVFNTVFQVSLGVLA
                     LLLTIAWIFAPYICRRWLAPGFDMEQHQLLVRFTRILLPAQLFFFAGGLFGAVQMARG
                     RHAFYAAAPLIYNAGIIAGGLLLAPRLGMEGFCWGALAGAVVGHGVLQFIGAWRSGLR
                     LQLRFTGFHPAVRHWMALTLPFILGQSILATDNWIQRYLGSGLSPASISWLNYGRNLM
                     LVLPAILGQAAAVASFPLLARQAEQGELGLMRSTLTRALERSLLLSGFGAVVLIVLNR
                     EVVQLAYGRGAFTTSDVLTTGHVLAILALAAPALVAQAIVARGYYALRDTWTPTIIGT
                     LITLGALPLYVYLSRWFDNPGFDRGGGHVGLAWASAGSLVLYGFLTILLLQRGLDRRD
                     PAAGKLIPGRFLLKCLPVLLVTLVIAALVRGAVGGWLPDMAFLPAIGRSVLVGSVALL
                     VFIVGCRLAGFREVLAPVAALLGRFGPKRVAGSEKVG"
     gene            8803..9939
                     /locus_tag="VF720_14560"
     CDS             8803..9939
                     /locus_tag="VF720_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880634.1"
                     /translation="MTDATDGKAGSTRQLLQFSAGYFLSYMATGILVKYFTAIRQPPM
                     SELAYLVNNTAGSSLLALGVVLALGWYRLKSNGKCDLLGFTVPVEYRYIIPSGICTAV
                     IIPTTTLMYLLPISVMVAMVLMRGSVIVISRLVDAIQIRQGILKKTVYAEENWAVLFA
                     ILAVATNVLFIPVVNALEARGVPAAAMFRISQKDRQGAFDFLSNGAALAIMGSYIIAY
                     AIRIYIMNYYKNTRAPGVPQDNKGFFAVEQIAATVTMFLAALVLFFASGLVGFSGPVV
                     EEFRAAIRSPAWDAVVSGIPWGIVAFFSVFLFMFQGRTATFAGLVNRLVSLLAGTAAT
                     LVLALVWGQSPPKPQDWVAFGFILVAVAFLSRAEKKRSMELARR"
     gene            complement(9974..11272)
                     /locus_tag="VF720_14565"
     CDS             complement(9974..11272)
                     /locus_tag="VF720_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEX7880635.1"
                     /translation="MTLPTTLVLFEDHAWRDLYPLTLVRPSFDLRVGVTTLGRRLSAQ
                     MAARKIMRTAYVCRPWLAPQVERTFPGAVVNELSGGDDLVFLNGAVLFFKGELERFLR
                     QLNDQAALESGGRLVAARVRAEDGRKLVAAITESYGPGPAESVPGAPSWPRGFKPKPV
                     TEPVRFLSRPWDLVHVNEATLIDDFALTRPGKEPALPAVSPGVHLVRKNRILSRPGSR
                     VMPGAVLDASTGPIILGEGVRILHNAVVLGPAYIGPNSLIKIGAKIYGGTSIGPTCKV
                     GGELEGSILHGHANKQHDGFLGHSYLAEWTNVGAGTDTSDLKNNYGRVKAWAPGGEVD
                     TGLTFAGLILGDHSKCGIGTMFNTGTVVGVMANIFGAGYPPKHVPSFSWGGAGSLESY
                     ELEKALAVAALVTARRQKVFEPIDEALYRKIFRDTAAHRR"
     gene            complement(11269..12978)
                     /locus_tag="VF720_14570"
     CDS             complement(11269..12978)
                     /locus_tag="VF720_14570"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX7880636.1"
                     /translation="MSTRLSRAASFRGFLLLAAALALLAPGEGMAAPAADLVFRGGSI
                     HTVDDSRPEATALAVKDGRIAFVGDDAGVAAFVGRKTRVVELAGRALVPGFVDADARL
                     LDVARLAQGVDLYATTSFADVTRKVAQAARAVPPNNWVIGWGWDEESWRDSEPIRLID
                     LQSVSGNRPVLLFHATDDAIIANVSALASASVPEDLPDPPGGRVARDMRGVRMGPLMG
                     EAASLVLRALAVPSTADFQRWYGAAAESCLVRGVTLVHDGTVPVSARSALSALWANEK
                     TRPALGLSVSLKPEPLEFDPVVSSPPGATDRVGGLSIEGVTFRLDGFVNTRGAAISHF
                     YYDSPGNRGFLRYSPAGLVPLAEGVRKAGWRPMFEAHGDSALSIALGTLALEDPAGQT
                     ARPLVSGLELIRPDQFARLAASGAGLVMTPTPMIDGWRWMDNRIGPEAVEQAWAMAEL
                     AKGSTLALGTGAPFADGRPILAFYAAVTRQNLDGRPAGGWKAEQGLTREAALRALTIG
                     GATLLGRDAETGSLAVGKRADLVVLSGDLMTVPEREIPALRVEMTVAGGRIVHDRAPA
                     VRN"
     gene            13148..>13618
                     /locus_tag="VF720_14575"
     CDS             13148..>13618
                     /locus_tag="VF720_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880637.1"
                     /translation="MSSSRSETIIDLKPFLARFRKIPVTPAHPDVTVDFGELVDDPPA
                     RPSLAGGRARGITTTWQLFGSLLALAVAIAAIITPLLLVLDPFLSRQHLLLAGLLIYS
                     ASILTATGAYWVVCGLVAGWLRRIEQEHATLPHRHAEGEPSPVHRLQATEIDRLL"
BASE COUNT         2261 a         4617 c         4634 g         2106 t
ORIGIN      
        1 acagaggaga tggtcgtctt cggccgggcg actgaatcga aatgagtccc tgccgtgcga
       61 aacggccagt gtgccctgct cgacatggat cgacagttcc cggccgtcgg ccattgcgag
      121 gatcagacga atgatacgac cgggcgccga ctctccccgc ggcccagccg aatcgatcca
      181 cgcccggaac gcgccgccgg tggttccgcg caggcgatcg cgcagccacc ccgcgaggag
      241 gcgcgggcgg acgggcatga cgcccgcgtc atccggatgc gactcgattc gcgcttcgcg
      301 gatcgtggcg acatcgatcg cgcagcgatc gaccgcatcg gccagcgcat cgcgccacgg
      361 gagcaggcgc acccaattca ggtcgatggg gcgcattccc gggccggcgg gtcgttccgc
      421 cagcgcgagc acattcgcga gatacgcctc cgggttgccg gcgagcgtgg agtcgaggat
      481 cacctggccg acataagggt cgaggtcgca caggaactgc gcgccgcggg gcagcggatg
      541 agggggcgga ccggccggtg ccatcatcga ggccacgccc ggccagtgaa gcacgacggg
      601 aaggtccgga aggaggagcg gcaggacgag gttcgaaagg tggggatcgc catccggggt
      661 cgagcggagg gtgatctgtt cggagcagac cgggcgtccg cgcgacgttg cgtgacaatg
      721 agcggaggcg aaggcggaga gcggttcggc cgccgtgcct ggatgatcgg tggccctttc
      781 ccctccccca tccagctcca caatcagcgc ccggcacggc gtctggcgtg tcaactcggc
      841 aatcatcgcc gtcattcgat cggccgcgac cttcccgtcg accaccgcca cgagcgtcat
      901 caacgcggcg cgtgtgattg gaccggtatc ggaaccgggc ctagcgccgg ggacacgggc
      961 caccgattcc ttccagaccg cattcagctc ttcttccagc atgcggaccg acaacgcgcg
     1021 ccctgccgtc gggaataccg tttcgctcac aggtgccgcc attcgcggcc gtcggcctgg
     1081 atccaccggt cggcctcggg cggaccccag tggcccgcgt cgtacggatg aagtggaatc
     1141 gaaggatccg cggcccaggc atcgaggatc ggctgcaccc aggcccacgc tccttctatc
     1201 tcgtcgcggc ggatgaagag cgacgggtcg ccgaggagcg catcgagcag caaccggtcg
     1261 taggcgtcgg gcacgtcggt accgaacgag ctgccgtaca ggaagtccat cttgaccggc
     1321 tggatcttga tgtcggggcc ggggcgcttg gcgccgaaac ggagcgcgat cccctcatcc
     1381 ggctggatgc gcagcgccag cacgttggcc gagaggccgt cggcacccac cgactcgaag
     1441 agctgctgcg gcggcgcctt gaactggatg gcgatctcgg tggcgcggcg gggcatcctt
     1501 ttcccgcttc gcacgtagaa cggcacgcct ccccatcgcc agttgtcgat ctcgagtcgc
     1561 gcggccagcc aggtttctgt tttcgactca gggttcacgc ccggttcggc ccggtaggcc
     1621 ggcaccgagg cgccgtcgat cgagccggcg gcgtactgcc cgcgcaccac ccgcgtggcc
     1681 acgtcggccg gcgcaagcgg cttgagcgcc cggatcacct tcaccttctc atcgcggata
     1741 ggctcacccc gatacggcac cggcggctcc atggccacca gcgagacgag ctgcatcagg
     1801 tggttctgca gcatgtcgcg gaggacgccg gccttctcgt agtagccggc ccgctgctcc
     1861 acccccactt cctcggccac cgtgatctgc acgtggtcga cgtggcgccg gttccagagc
     1921 ggctcgaaga tgccgtttcc gaaccggagc accaggatgt tctgcaccgt ctccttgccg
     1981 aggtaatggt cgatgcgata gatctgcgac tcgtcgaaga tggtgctgat gagccggttc
     2041 agggcccgcg ccgactccag gtcgtgcccg aacggcttct cgacgaccac ccgggcccag
     2101 ggggactgcc gggcctcgcg cggcacgagc tgcgactgcg ccagtccctc gaggatcggc
     2161 gcgaaggcgg ccggcggcgt ggccaggtaa aagagccggt tcgaggggcg attggcggcc
     2221 gactcgattt ccgcaagcct cttgccgagc tgcggccacg tttccttcgt ggcgaagtcg
     2281 ccgccgatgt aatgaaagag cgtggcaaac tcccgggcct cgggggattg cacggcatcc
     2341 tcacccacat gctcggcaat ggccttggtc acctccgcgc ggaacgcggt atcgtccagc
     2401 tccgtacggc tgacacccat cacaaggaag cccggcggca ggtggcggcg ctggaagagg
     2461 cggaagaccg ccgggacgag cttgcgccgg gtgagatccc cggtggcgcc gaagatgacc
     2521 agtgtggcgg ggtcgggcat gcgttcgcgg ggaagtccct cgcgcagggg attagggagt
     2581 gtgggcatgt cggacgagcc tagcaggccc ggccggcggt gcaagtccgg ccgcccacga
     2641 agggttccgg cgccccagtc tgacgggcgt cacgaaatcg tcacagcgac gtcaacggat
     2701 cgggacgaat ccaaggcatt ttgatgtcgc gtgtcatgga tctgtcgccg cctgctcacc
     2761 cgggctggcg gcaaccgcca cacgggcggc cgggcaccgg gatcatcaag ggggtgtttg
     2821 ttgcggttga tcgcgcgatg gatgggagcc ctgggcctga gcctgctcct tgcctccccg
     2881 gccctcggcc aggaggcgga ctcggtggga accacgtccg acgaactgtc cgggaagctc
     2941 gaaggggtca tcgaacaact tcagactctg caatccgaca ccgacaagct caagaagatc
     3001 aaggtgacgg ggtacgtcca ggcccgctac gaatcgagcc aggccagcga cgattcggtc
     3061 cgggccgccg gcaacccgct ggtgctgacg ccggccaacc tcgaccgctt ctacatccgg
     3121 cgggcccggc tgaaggtaac gtacgatccg agcgagcgga cgcaggcagt ggtgtacttc
     3181 gacggcgggg ctgaccggca gatccgcctc ctcgaggcct atgtccagat gtccgacccc
     3241 tggacggtcg accagaagca catcctcacg atcggccagt tcaacatccc gttcggatac
     3301 gagatcgaac gctcctcgtc ggtgcgcgag ctccccgagc gttcccgggc cgagaacgtc
     3361 ctgtttcccg gcgaacgtga ccgcggagtc aagctcgtcg gccggtggag tgaccgcctc
     3421 gagaccgtgg caggcgtgct gaacggcggc ggcatcaacc aggtcgacta tcccaacacc
     3481 gacccgaccg ccgccaagga cttcgtgggg cgggcgcgat tgagcttcgg gaaattcgac
     3541 gtggccgcct cggcctccgt cggcgagcac gtgatcggcc tgaccggccc cgacgtgaag
     3601 accgaccgca cccgcttcgg caccgacgcg cagggttact gggaactgcc gaaggcgggc
     3661 ggcggcacgc tgcgcgggga agtatacgtg ggtgaggagt tcaaccccga ctcggtggcg
     3721 gtgctcacga cgcgtcccac gaacacggcc cccgtgcgat tcctgaagcc gggcgcggag
     3781 gccgcgcacc tcgactccga gttcctcggc tggtacgtga tgctggtgca gaacctcggc
     3841 gaccggttcc aggccgcggc gcgctacgac gtgtacgacc ggaacaccga cgtggacggc
     3901 gacgactacc gccgctggaa tgtcggcctc aactggttct acggcggcta cacgcgcttc
     3961 acgctctcgt acgaaagccc ggacgtggag tccacggccg gtgcccccgt ccccaacccc
     4021 gacgacaacc tgctgacctt ccaggcgcag ttcaagttct gaggagattg tcatgactcg
     4081 gcacactatc ctgcccgccc ggtggcgccg gatcgccggc ctcgccctgc tcgggctctc
     4141 cctcgtgacg tgcgtggcct cggccgccgg caaggccatc acgatcaagg gttccgacac
     4201 aatggtgatc ctgggccagc gctgggtgga gcgctacatg gccacgcatc ccggcagcgt
     4261 gctccagatc accggaggcg gctcgggaac cggaatcgcc gccctgatca acggcaacac
     4321 cgacatctgc caggcgtcgc gcccgatgaa gaccgacgag aagaagaagc tgcgcgaccg
     4381 ctaccagacg atgggcgtgg agatcccggt ggcccgggat ggcctgtcga tctacgtgaa
     4441 cgaggccaac ccgatccagg aattcaccat ggagcagctg cgcggcatct acacgggcca
     4501 gatcctcaac tggaaggagc tgggcggggc cgacgagccg atcgtcgtct attcgcgcga
     4561 gtcgagctcg gggacctatg tctttttcaa ggaccacgtg ctcggcggcg ccgatttcgc
     4621 gccccaggta cagacgctcc ccggaaccgc cgccatcgtc aacgccgtgt cgaaggacaa
     4681 gcggggcatc ggctacggcg gcgtggcata cgcgaagggg atccgggtgc ccgcgctcaa
     4741 gaaggatgcc gccgctcccg ccatcctgcc gaccgaggcc gccgtgaagg acgggagcta
     4801 cgccctttcc cgcgatctct atttctacac gcgcaagaag cccgacggcg cggtgaagga
     4861 attcatcgac tgggtgctgt cagcggaagg gcagaaactg gccattgatg tgggttactt
     4921 cccggtgaac tgacactggg tcgcaaacag gttcatcccc cggcccgaac tccgccggca
     4981 tccggtacac tcgccggatg ccggcctttt tttcggtggt cctggccacc tggaatcgcg
     5041 cgccccgcct ctcgaaagcc atcgattccg tcctcgccca gaccgatgat gattgggagc
     5101 tgatcgtcgt cgatgacggt tcgacggatg acaccgccgc cgtcgtcgaa tcgtaccggc
     5161 ggaatcgccg cgtcgtgtgg gtcccacgcc cgcacgaagg ggtgtggccg gccaagaacg
     5221 ccggcgcggc cgaagcccgc ggtcgatgga tcacctttct ggattcggat gacgcttaca
     5281 tgccggagca cctggcggcg cggaggcgct gcatcgtggc tggcccggag attcgtttct
     5341 ggcacggcgg ggtgacggtg cttggcccgc ccgagatgca ctcggtgccc gacgccgacg
     5401 atccaacccg gctcattccc ctggccgatt gcgcgatcgg ggcgtcgttc gtggtgaagt
     5461 caacgtggtg ggcccggctc ggcggattcg accctacgct ctgggcggcc gacaccgatc
     5521 tcctgaaacg ggccgcggct ctggagagtg gcgctatcgc gcggtgcgcc gaaccgacct
     5581 atctgtacat ccgcacaccg ggagacggcg tgtgcgaagc cgagcggctc cggactagat
     5641 aggcatcacc acgagcatga gcgcgcgggc gaacgggcga cgggaggctt ccggcacgtt
     5701 accgttcgag aggacgccga aggcctggtc ttcggccagt ccgtagaact ccgcgtaggc
     5761 cccggccact ttctcggccg tgtcggcgcc gctgagaaag atcatgagat tgtcctgcgc
     5821 gccccctccc gggaggacca gctgggtgtg cgccagctgc tcattggctg ctgtgccggg
     5881 aaaagccgcc gccatcacgt tgacgacggt gttctcgtac gcattgaact cggccagtat
     5941 cgccgcatcg ctcccgattc cgtacatgct gttgcggagc gtgacgccgg ccatggccac
     6001 cgtggaaaga cccgccggag aaaccccggc catctcgagg agcgtgtcga cggccgccgc
     6061 catcgtgcgc gcgcgctgga gcgcggcggc ttcgctcatc acccgcttca ggtcgctgcg
     6121 ggcgctcgcc gcccggcgcc ggtattcgcg cattcccgcc tcgcgcgcca cggcaagcac
     6181 ctcggggcgg atgccgttca tcgtccaggc ctcgcgttcg cgcacgaacc aggcctcgtg
     6241 agcatggatc gttcgggcct ggttttcgtt tgaggggtcg agagcggtgt cgagatcctc
     6301 atccagccgc accagcgcgg cccggcgggc cgtgcgggcc gcggcgagcc cgatggcgct
     6361 cgttcccact ttctcctcga gatgaaaccg atcgaccgcc tcgacctggc cggcgatggc
     6421 atcccccgcg gaggccacca gctcggcgcc ggtgatcacg atggccgcca ccgacgagtt
     6481 cacgtcgagc gacacatcgg gccgcggtat cgaccccagt tgcggatgat cctgcagcca
     6541 ggcccagatt tctgcctcgc cggtcgtctc gtcgttgagc ggcgccacaa cgacggtcga
     6601 gtcggcccgc accgggcgcg acaccagtgc cttgtaacgc tcattgtcgc ggaccgccgc
     6661 cacgacgagg tccgcttccc cgtcggcgtt caccgacacc acgaaccggc cgttctggtc
     6721 cgacaccgcg gtgtcggccg tcacgtcgat gaagttgccg tcgtcgtcga tccgctcgac
     6781 ggtgacgacg gcgccgacga ggtgggtcgg cgagggcaac ccttccggcg ccaaacgatc
     6841 tggcgccccg atgtagccgc cgccccgctc gatctggccg cggatcatgg ccaccggttc
     6901 cggatcgggt ggatcgtcgt cgtcgccaca ggaggtcagg aacatggccg caaggaagag
     6961 cgtgatcagt tgaagcggcc gcgaaggcgg ccgcaacgac atgacggcag gcccaacggt
     7021 ggcttttggt ggcaacggtg gcgcgacggc ggacatgatg tcggtcacgt tacccgacct
     7081 tttctgaccc ggcaactcgc ttcgggccga aacggccgag aagcgcggcc accggggcca
     7141 gcacctcacg gaagccggcc aggcggcagc ccacgatgaa gacgaggagg gccacgctcc
     7201 cgaccaggac cgaccgtccg attgccggga ggaaagccat gtcgggaagc caccctccga
     7261 ccgctccacg caccagcgcc gcgataacga gcgtgaccag cagcaccgga aggcacttga
     7321 gaaggaatcg ccccgggatg agcttgcccg ccgccggatc gcgccggtcg aggccgcgct
     7381 gcagaaggag aatcgtgagg aatccgtaga gaacgagcga gccggccgaa gcccacgcga
     7441 gacccacgtg tccgcccccc cggtcgaagc cgggattgtc gaaccatcgc gacaggtaca
     7501 cgtagagcgg cagcgcgccc agggtgatca gggtcccgat gatcgtgggt gtccaggtat
     7561 cacgcagcgc gtagtagcca cgcgcgacga tggcctgcgc gaccagcgcg ggagcggcca
     7621 gcgccaggat ggccagcacg tgtcccgtcg tcagcacatc cgaggtcgtg aaggccccgc
     7681 gaccgtaggc gagctgcacc acctcacggt tcagcacgat gagcacgacc gcgccgaatc
     7741 ccgagaggag cagtgagcgc tcgagcgccc ttgtcagggt cgagcgcatc agccccagct
     7801 ccccctgctc ggcctgccgc gccaggagcg ggaacgacgc caccgcggcg gcctgcccca
     7861 ggatggcggg aagcacgagc atcaggttcc ggccgtagtt gagccagctg atggaggccg
     7921 gtgacagtcc cgatcccagg tagcgctgga tccagttgtc ggtggccagg atcgactggc
     7981 cgaggatgaa cggcagcgtg agcgccatcc agtggcggac ggctggatgg aagccggtga
     8041 agcgaagctg caaccggagg ccggaccgcc aggcgccgat gaactgcagc accccgtggc
     8101 cgaccaccgc cccggccagc gcgccccagc agaacccctc cattcccagg cgcggcgcga
     8161 gaaggaggcc gccggcgatg atccccgcgt tgtagatgag cggcgcagcg gcatagaagg
     8221 cgtggcgccc gcgggccatc tgcacggcgc cgaagagtcc gcccgcgaag aaaaataact
     8281 gggccggcag caggatgcgg gtgaaccgca ccaggagctg atgctgctcc atgtcgaacc
     8341 cgggagccag ccatcgacga cagatgtagg gcgcgaagat ccaggcgatg gtgaggagca
     8401 gggccaggac gccgagcgac acctggaaga cggtgttgaa gaccttccat ccccgctctt
     8461 cctccttgcg gatcatgaag ccgaggaaga tcggaatgaa ggtgaccgag agcgtaccgc
     8521 cggccaccag gttgttgacg atgtcgggga ggttgaaggc ggtgaagtag acgtcggtgt
     8581 ggccgtcggc gccggccatg cgggcgatga ccatttccct gagcagcccc atcacccggg
     8641 agagggccac ggccaccatc cagatcagcg tggcgactcc catttgtcgc cgggtggcct
     8701 cggtcatcgg agcggcatct taagcacccc atgaccggcc cgcaaccgcg caactaccgt
     8761 tcttttctgg acttgaaggg gaaacaggtt taacgtctcc cgatgacgga cgccacggac
     8821 ggcaaggccg gctccacccg gcagctcctg cagttctccg ccggctactt cctgtcgtac
     8881 atggccaccg gcatcctggt gaagtacttc actgctatcc ggcaaccgcc gatgtccgag
     8941 ctggcctacc tcgtcaacaa cacggccggg tcgagtctcc tggcgctcgg cgtggtgctc
     9001 gcgctggggt ggtaccgcct caagtcgaac ggcaagtgcg acctcctcgg attcaccgtc
     9061 ccggtcgaat accggtacat catcccgtca ggcatctgca cggcggtcat catccccacc
     9121 accacgctca tgtacctgct cccgatttcc gtgatggtgg ccatggtgct catgcgcgga
     9181 tcggtgatcg tgatctcgcg cctggtcgat gccatccaga tccgccaggg cattttgaag
     9241 aagaccgtgt acgccgagga gaactgggcg gtgctcttcg ccatcctggc cgtggccacc
     9301 aacgtgctgt tcattccggt cgtgaatgcg ctcgaggcgc gaggcgtgcc ggcggcggcg
     9361 atgttccgca tctcgcagaa ggaccggcag ggtgcgttcg acttcctctc gaacggcgcg
     9421 gcgctggcca tcatggggtc gtacatcatc gcctacgcca tccgcatcta catcatgaac
     9481 tactacaaga acacccgggc gcccggcgtg ccgcaggaca acaaggggtt cttcgcggtg
     9541 gagcagatcg ccgccaccgt cacgatgttc ctcgcggcgc tggtcctctt ctttgcctcc
     9601 ggcctcgtgg gcttctccgg cccggtcgtt gaggagtttc gcgcggcgat ccgctccccc
     9661 gcctgggacg ccgtcgtctc cggcatcccc tgggggatcg tggccttctt ctcggtcttc
     9721 ctcttcatgt tccaggggcg tacggccacc tttgccggac tcgtgaaccg gctggtgagc
     9781 ctgctggccg gcacggcggc cacgctggtg ctcgcgctcg tatggggcca gtcgcccccg
     9841 aagccgcagg actgggtggc cttcggtttc atcctggtgg cggtggcgtt cctctcgcgg
     9901 gccgagaaga aacgttcgat ggagctggcg cggcgctagc taccgccaga ccggcctcag
     9961 cgacagggcg gcgctaccgg cgatgtgccg cggtgtcgcg gaagatcttc cggtagagcg
    10021 cctcgtcgat cggctcgaac actttctggc gacgcgcggt gacgagcgcc gccaccgcca
    10081 gcgccttctc gagttcgtac gactcgagac tcccggcgcc gccccagctg aacgatggca
    10141 cgtgcttcgg cggatacccg gcgccgaaga tgttggccat cacgccgacc accgttcccg
    10201 tgttgaacat cgtgccgatg ccgcatttcg agtggtcgcc caggatgagg ccggcaaacg
    10261 tgaggcccgt gtcgacctcg ccccccggcg cccaggcctt cacacggccg tagttgttct
    10321 tgagatcgct ggtgtcggtg ccggcgccga cgttggtcca ttcggccagg tacgagtgac
    10381 cgaggaagcc gtcgtgctgc ttgttggcgt ggccgtgcag gatcgatccc tccagctcgc
    10441 cccccacctt gcaggtgggc ccgatcgagg tgccgccgta gatcttcgcg ccgatcttga
    10501 tgaggctgtt cgggccgatg tacgccggcc ccagcaccac cgcgttgtgg aggattcgca
    10561 ccccttcgcc gaggatgatc ggcccggtgg aggcgtcgag gacggcgccg ggcatcacgc
    10621 ggctgcccgg acgcgagagg atgcggttct tccgcaccag atgcacgccg ggcgacacgg
    10681 cggggagcgc gggctccttg cccggccgcg tcagcgcgaa gtcgtcgatc agcgtggcct
    10741 cgttgacatg cacgaggtcc cacgggcgcg acaggaagcg gaccggctcg gtgaccggct
    10801 tcggcttgaa gcctcgcggc cacgagggcg cccccggcac tgattcggca gggccggggc
    10861 catacgattc ggtaatggcg gccaccagct tcctgccatc ctcggcgcgc acgcgggcgg
    10921 ccaccagtcg gccgccgctt tcgagggccg cctggtcgtt cagctggcgc aggaaccgct
    10981 cgagctctcc cttgaagaag aggacggcgc cgttcaggaa gacgaggtca tcgcccccgg
    11041 aaagctcgtt gacgaccgca cccggaaacg tccgctcgac ctgtggcgcc agccatggcc
    11101 ggcagacgta cgccgtgcgc atgatctttc gcgcggccat ctgggccgag aggcgacgcc
    11161 cgagcgtggt gacgcccacg cgcaggtcga acgagggacg gaccagggtg agcgggtaga
    11221 ggtcccgcca ggcgtgatct tcgaagagga cgagggtggt cggcagtgtc agttccgcac
    11281 ggccggcgcc cggtcgtgga cgatgcggcc gccggccacg gtcatctcca cgcggagcgc
    11341 cggaatttcg cgctccggaa cggtcatgag gtcgccggag agtacgacga ggtcggcccg
    11401 cttgcctacc gcaaggctcc ccgtctcggc atcacggccg agcagcgtgg cgccgccgat
    11461 cgtgagggcg cgcagcgcgg cctcgcgcgt gaggccctgc tcggctttcc atccgccggc
    11521 cggccggccg tccaggttct ggcgggtaac ggcggcgtag aacgccagga tcggccgacc
    11581 atcggcgaag ggtgcgccgg taccgagcgc gagggtcgag cccttcgcga gttcggccat
    11641 ggcccaggcc tgctccaccg cctcggggcc gatgcggttg tccatccagc gccagccgtc
    11701 gatcatcggc gtgggtgtca tgacgaggcc ggcacccgag gcggccagcc gagcgaactg
    11761 gtcgggtcgg atgagctcga ggccgctcac cagggggcgc gcggtctggc cggccgggtc
    11821 ttcaagggca agtgtcccga gggcgatcga gagcgcgctg tcgccgtgcg cctcgaacat
    11881 cgggcgccac ccggcctttc ggacaccctc cgccagggga accagccccg ccggggagta
    11941 acggaggaag cctcggttgc cgggcgagtc gtagtagaaa tgggagatcg ccgcgccgcg
    12001 cgtgttgacg aacccgtcga gccggaaggt gactccctcg atcgacagac ccccgacacg
    12061 gtccgtcgct ccgggagggc tcgataccac cgggtcgaac tccagcggct cgggcttgag
    12121 gctcaccgaa aggccaaggg cggggcgggt cttctcgttt gcccagagcg ctgacagggc
    12181 ggaacgcgcc gacacgggta ccgtgccgtc atgcaccagc gtcacgccgc gtacgaggca
    12241 gctctcggcc gccgcaccat accagcgctg gaagtcggcg gtcgatggca cggcaagcgc
    12301 ccggaggacc aggctggccg cctcgcccat gagcggaccc atccgtacgc cccgcatgtc
    12361 gcgggccacc cggccaccag ggggatcggg aaggtcttcg gggacgctgg ccgaggcaag
    12421 tgccgacacg ttggcaatga tggcgtcgtc ggtggcgtgg aagaggagaa ccgggcggtt
    12481 tccgctgacc gactgaaggt cgatcagacg gatcggttcc gaatcgcgcc acgattcctc
    12541 gtcccatccc cagccaatca cccagttgtt gggcgggacg gcccgggcgg cttgcgccac
    12601 cttccgggtc acgtccgcga agctggtggt ggcgtacagg tcgactccct gcgcaagccg
    12661 ggccacgtcc aggagtcggg cgtcggcatc gacgaagccg gggaccagcg cccggccggc
    12721 gagctcgacc acccgggtct tcctgccgac gaacgccgcg actccggcgt catcacccac
    12781 gaaggcgata cggccgtcct tgacggccag ggcggtggct tcggggcggg aatcgtccac
    12841 ggtatgaatc gatccgccac gaaacacgag atcggcggcc ggggcggcca tgccctctcc
    12901 cggtgccagg agggcgaggg cggcggccag cagcaggaaa ccgcggaacg aggccgcgcg
    12961 ggacaggcgg gtggacattt cgatcgaatc ggtcaggcga aacctccgtt tgtcaggcca
    13021 tggcggcggg cattctagca ggccccggcg ccgtgatttt ggctcagtcg aggggtggaa
    13081 ctgtcgataa ccgatccacc tcctggccgc ccactcgagg cgtgccggcg ttggacggag
    13141 gatggccgtg tcgagttccc gctccgaaac aatcattgac ctgaagccgt tcctggcgcg
    13201 cttccggaag atccccgtga cgccggcgca cccggacgtc acggtggact tcggcgaact
    13261 cgtggacgac ccccccgccc gaccctccct ggctggcggt cgagcccgcg gcatcaccac
    13321 cacctggcag ctcttcggca gcctcctcgc actcgccgtg gcgattgccg ccatcatcac
    13381 gccgctcctg ctcgtcctcg atcccttcct ctcgcggcag catcttctcc tcgcggggct
    13441 tctcatctac agcgcctcga ttctgaccgc aaccggcgcc tactgggtgg tctgcggcct
    13501 ggtggccgga tggctgcgcc ggatcgaaca ggagcacgcc acgctccccc atcgccacgc
    13561 ggaaggggag ccgtccccgg tgcaccgcct gcaagccacg gagatcgacc ggctgctc
//
LOCUS       DASVOD010000681         7829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_151396, whole genome shotgun sequence.
ACCESSION   DASVOD010000681 DASVOD010000000
VERSION     DASVOD010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151396"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VF720_14580"
     CDS             complement(<1..785)
                     /locus_tag="VF720_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880638.1"
                     /translation="MNRLRLLVLVGVLAFVANVTSLGNGFVYDDGVIIEENPRVLDAT
                     KMGDILTTPYWGSRAGGGLYRPVATFTYALNHRAGGLNPFGYHLVNVLLHACVSVLVA
                     LVAASFLPIWFAALAGALFAVHPIHVEAVANVVGRAELLAAFGFIGAWLAARRARQAG
                     GARSDGGGRSTSGSIAWRLIAAFLYGVALFSKEVAVGLPVALLVADWLERRRPDWLLS
                     AFLFGMLGLYLFARWKVLGSVGLPKEFVIYRLDNVIATQPFLPG"
     gene            complement(782..2344)
                     /locus_tag="VF720_14585"
     CDS             complement(782..2344)
                     /locus_tag="VF720_14585"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7880639.1"
                     /translation="MMPRSMSRFVALCLVAGCLAAVFVSAGCGGKAPAPRVFVIGLDG
                     ATFDLLKPWLDKGELPNLKRLMDSGVWGELASVNPIISPVAWTSAATGVNPGKHGIFG
                     FERPDPTHPGAQLLNTSEQRKAIPVWTLLGNAGYSVGVLNVPMTWPPDPVNGVMVSGF
                     PFPESLGTSPYTSPAGLAPSLGDYPLDKMGEGLVTGAEGDMLARIVQSRDAVVRVFLD
                     WTKTRKDDFTWAVVTATDRVQHFFWGMMDEKHPYWTPERGAEFGSAIHEFWLAMDKRL
                     GEMLAALPEDSIVLVISDHGFAPIYREMNMYNWFTGTQLDEYVKEHRVPDVFITNGIF
                     NYRLGTTWPMSPAYDEFRNLFEAEALGVVDPKTGLKPVERVARREQIFGGWQIGKAPD
                     LVMVEAPNYYIGAGDPSKPLEPVTDLHSTSYSAYHRPNGIFVISGPNIGKGNLAGASL
                     LDVAPTLLHLMHQPVPSEMDGRVLTAIFDPAWLSKNAPKYSDGMKILADRPERELTEE
                     ERRRLAAVPYIR"
     gene            complement(2394..3926)
                     /locus_tag="VF720_14590"
     CDS             complement(2394..3926)
                     /locus_tag="VF720_14590"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX7880640.1"
                     /translation="MRRASIGFLAALLALGVFAPACNGPSRKPAALVIGLDGATWKLL
                     RPWMAAGELPNLKGLIDSGVSGDLISVVPFLSPPAWTSAVTGVNPGKHGIYDFLRRLP
                     GEMTMVNETSRSRRVPAIWQLLSTAGVTVGVVNVPASDPPDQVNGFMISGMPHVDVTD
                     YTWPKSLASELTNYKIDRMDLSIQPGREQELLDNLLTTMRARAANIEHLLATREWEFA
                     WVVFTETDRIQHFFWQFMDPEWPDYNAAKAARYGTAIHDLWVELDGYLGKIVAAARAK
                     HGENLPILLVSDHGFGPVHREFRVQSFLRNPTTGEAPITEAYAVETNGAYLYFSRAGR
                     EPNGALSREDFDRTRNDAMARIKSTRDPDSGLPPMRAIFKNEQIYSGPFREKGPDLAM
                     VPAPGVYISNDRGFREPWGTPSYTFSGHHEMEGILIATGGPFRAGKLDQGVSLLDITP
                     TLLYLMNQPVPDNVDGKVLTEILDPDFVKSHVRKTVAAGGPIGTDSVDVRSRLNSLPY
                     VH"
     gene            complement(3942..4757)
                     /locus_tag="VF720_14595"
     CDS             complement(3942..4757)
                     /locus_tag="VF720_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880641.1"
                     /translation="MFLISPGKRCIGIVERPDSRPAFRPSILPALPLLIGLSGALLSA
                     GPALAQYGTPNEEAKIIIHVVPVTSMSGRACSSNKAQAPCDQMVTNGKVNEYYYGFVC
                     VVDGKRDAGIAGVTFGVKFNRTPRSGVDISEWTRCGTLEFPTTSGGRRDWYNVSNGGN
                     LVTWDPQNACQTTEPSGSGTGVTAVAGYFYLTAYSADRLEIVPRPADNQAAVADCTSI
                     LSIVAGPGAPIHTKPYVGTVEFSADGSVVGSNPCGLAHPVVGKTWTGVKQSGR"
     gene            complement(4932..5693)
                     /locus_tag="VF720_14600"
     CDS             complement(4932..5693)
                     /locus_tag="VF720_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880642.1"
                     /translation="MRIGRPLRRIGSLVVSLAAFAAFALLLFMPDLALAAGGTEPQPR
                     SNEAGNVLLPELCTVPRCLQIHPSTLIANALPNHGAKYNVTIVGNGGPIASANIQIRL
                     LTPGDTLACWCNDTPGPRPHVFSQSTNVQGQARFVIGGGGCIEYGLAAIPGVLDYAGE
                     VFADGIRLQEFGVVSSDAVDNAGRRATDSPRWNPAGTCGAGITDAVEHTGPLGNNSYD
                     WCSDFNCDSATGAADAVIVTPFLAGAASCPGTSGP"
     gene            complement(5791..6519)
                     /locus_tag="VF720_14605"
     CDS             complement(5791..6519)
                     /locus_tag="VF720_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880643.1"
                     /translation="MTKAAITFLATALFGLSSALLMTRPAEAAGNRDAKIMLHALSTT
                     SKNPCVRAANLPANCVGFNAGINNLPLYPTTHYTYLLVVDGSQVEGIAGVQCGIDYNS
                     AFGQGVDVYGWTLCASLEFSSTNWPQDGGGNLITWDATTRCQTSGNAALGAVGVAGYF
                     YMAAYTPDIMRVTRRPVDNLAKVANCGAAEDIIQVCYRPETDESTFLGAVGFGRTGVN
                     PCGRLCGLPVEAASWSEVKALATD"
     gene            complement(6606..7334)
                     /locus_tag="VF720_14610"
     CDS             complement(6606..7334)
                     /locus_tag="VF720_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880644.1"
                     /translation="MSRILRSVVLAGVATLAIATGAHAAGNASAKIMLHSLTTTTKNP
                     CTRATNVPAACGGYDNGVSNQTLTSGTPAFHYVYLLVVNGSQVEGIAGVQCGINYNGA
                     EFVGVDVFTWSLCATLEFASTGWPNAGGGNLITWDATTRCQTAGNATIGAVAVAGFFY
                     MGAYTPDVMRVTPRPVDALAKVANCGSVEDIVFSPAGDDAHTFLGAVGFGPGNTGINP
                     CGRELVLPVEPTSWSAVKTLTSTN"
     gene            complement(7470..>7829)
                     /locus_tag="VF720_14615"
     CDS             complement(7470..>7829)
                     /locus_tag="VF720_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880645.1"
                     /translation="WPQDGGGNLITWDATTRCQTSGNALIGTVAVAGYFYMGAYGNDV
                     MRVTPRPVDGFAKVANCGSVEDIPWGGAPGDVATPGDTFLGAVGFGQSSGINPCGRER
                     PVPTVDATWSGVKSLLH"
BASE COUNT         1352 a         2555 c         2690 g         1232 t
ORIGIN      
        1 cccggcagga agggctgtgt ggcgatcacg ttgtcgaggc ggtagatcac gaattccttc
       61 ggcaggccga cgctgccgag caccttccac cgcgcgaaca ggtagaggcc cagcatcccg
      121 aagaggaagg ccgacaggag ccagtcgggc cgccgccgct cgagccagtc ggccacgagc
      181 aacgccaccg gcagccccac cgcaacctct ttcgaaaaga gggccacgcc gtagaggaac
      241 gcggcaatca atcgccaggc aatgcttccg gaagtcgatc gcccgccccc gtccgaccgc
      301 gcccctcccg cctgccgcgc gcgccgggcc gccagccacg caccgatgaa gccgaacgcg
      361 gccaggagct cggcccgtcc caccacgttg gccacggcct ccacgtggat ggggtgcacg
      421 gcgaagagcg cgccggccag cgcggcaaac cagatcggca ggaacgaggc cgccacgagc
      481 gccaccagta cgctcacgca cgcatgcagc agcacgttca cgaggtgata accgaacggg
      541 ttcaggccgc cggcgcgatg gttgagcgca taggtgaacg tggccaccgg ccggtagagc
      601 ccgccgccgg cgcgactccc ccagtagggc gtcgtgagga tgtcgcccat tttcgtggca
      661 tcgaggaccc ggggattttc ctcgatgatg acgccgtcgt cgtaaacgaa gccgttgccg
      721 aggctggtga cgttggccac gaaggccagg acgcccacca ggaccagcag ccggaggcgg
      781 ttcaacggat atacggcacg gccgccagcc ggcgacgctc ttcttccgtg agctcccgct
      841 cggggcgatc ggcgaggatc ttcatcccgt cgctgtactt cggcgcgttc ttcgagagcc
      901 acgccgggtc gaagatggcg gtgagcaccc ggccgtccat ctcgctcggg accggctggt
      961 gcatgaggtg caggagcgtg ggcgccacgt cgagcaggct ggcgccggcc aggtttcctt
     1021 tgccgatgtt cgggccggaa atcacgaaga tgccgttcgg ccggtgatag gccgagtagc
     1081 tggtggaatg gaggtcggtg accggctcca gcggcttcga gggatccccc gcgccgatgt
     1141 agtaattggg cgcctccacc atcacgaggt cgggggcctt gccgatctgc cacccgccga
     1201 agatctgttc gcgccgcgcc acccgctcca ccggcttcaa cccggtcttc ggatcgacga
     1261 cgcccagcgc ctcggcctcg aagaggttgc ggaattcgtc gtaagccggg ctcatcggcc
     1321 aggtggtgcc gagccggtag ttgaagatgc cgttggtgat gaagacgtcg ggaacccggt
     1381 gctctttcac atactcgtcg agctgcgtcc cggtgaacca gttgtacatg ttcatttcgc
     1441 ggtagatcgg cgcaaaaccg tggtccgaaa tgaccagcac gatggaatct tctggcaacg
     1501 cggccagcat ctcgcccaac cgcttgtcca tggccagcca gaactcgtgg atggcgctgc
     1561 cgaactcggc gccccgctcg ggcgtccagt agggatgctt ctcgtccatc atcccccaga
     1621 agaagtgctg cacgcggtcg gtggcggtga cgaccgccca ggtgaagtcg tccttgcgcg
     1681 tcttcgtcca gtcaaggaac acccgcacca ccgcgtcgcg gctttgcacg atgcgggcca
     1741 gcatgtcgcc ctcggcgccc gtcaccagcc cctcccccat cttgtcgagc ggatagtccc
     1801 cgagcgacgg cgccagcccc gccggcgagg tgtaggggct cgttcccaat gattcgggaa
     1861 acgggaagcc cgacaccatg acgccgttca ccgggtcggg gggccaggtc atcggcacat
     1921 tcagcacgcc cacgctgtag ccggcgttgc cgaggagcgt ccagaccggt atggccttcc
     1981 gctgctcgga cgtgttgagg agctgcgcgc ccggatgcgt gggatcgggg cgctcgaagc
     2041 cgaaaatgcc gtgcttgccg ggattgaccc ccgtggcggc ggaggtccag gccaccggcg
     2101 agatgatcgg gttcacgctg gccagctcgc cccacacacc cgaatccatc agccgcttga
     2161 ggttggggag ctcccccttg tcgagccacg gcttcaggag atcgaacgtg gcgccgtcga
     2221 ggccgatgac gaagacgcgc ggggcagggg ccttgccgcc gcagccggcg gacacgaaca
     2281 ccgcggccag gcagccggca accaggcaga gggcaacgaa tcgagacatc gaacgtggca
     2341 tcatgcgcgc cggattctac ccggaaccgt ccggcgctcc aacaaagcac ccatcaatgc
     2401 acgtacggca ggctgttgag ccgcgaccgc acgtccacgg agtcggtccc gatcggcccg
     2461 ccggccgcca ccgtcttccg gacatggctc ttcacgaaat ccggatcgag gatctcggtg
     2521 agcaccttgc cgtccacgtt gtcgggcacg ggctggttca tgaggtagag aagcgtcggc
     2581 gtaatgtcga ggaggctcac tccctggtcg agcttgccgg cgcggaacgg ccccccggtg
     2641 gcgatgagga tcccttccat ttcgtggtgg ccggagaagg tgtacgacgg cgtgccccac
     2701 ggctcgcgga agccgcgatc gttcgagatg tagacgccgg gcgccggcac catggccagg
     2761 tcgggcccct tctcgcggaa cgggccggag tagatctgct cgttcttgaa gatggcgcgc
     2821 atcggcggca gccccgagtc gggatcgcgc gtggacttga tccgggccat cgcgtcgttc
     2881 cgggtgcggt cgaagtcctc ccgcgagagg gcgccgttcg gctcgcggcc ggcccggctg
     2941 aaatagagat aggcgccgtt cgtttcgacc gcgtaggcct ccgtgatcgg cgcctcaccg
     3001 gtagtcgggt tccggaggaa cgattgcacc cggaactcgc ggtgcaccgg cccgaagccg
     3061 tggtcgctca cgaggaggat cggcaggttc tccccgtgct tcgcgcgcgc ggccgccacg
     3121 atcttcccga ggtagccgtc gagctccacc cagaggtcgt ggatggcggt accgtagcgg
     3181 gcggccttgg cggcgttgta atcgggccac tccgggtcca tgaactgcca gaagaagtgc
     3241 tggatgcggt cggtctccgt aaacaccacc caggcgaact cccactccct ggtcgccaac
     3301 aggtgctcga tgttggccgc gcgggcccgc atcgtcgtga ggagattgtc gaggagttcc
     3361 tgttcgcgac cgggctggat ggaaaggtcc atccggtcga tcttgtagtt cgtgagctcc
     3421 gatgccagtg atttcggcca ggtgtagtcg gtgacatcca catggggcat cccggagatc
     3481 atgaagccgt tcacctggtc ggggggatcc gaggcgggca cgttcaccac gcccaccgtc
     3541 acgccggccg tcgacaggag ctgccagatg gcgggcacac gccggctccg cgacgtttcg
     3601 ttcaccatgg tcatctcccc cggcagccgg cgcaggaagt cgtagatgcc atgcttcccc
     3661 gggttcacgc cggtgaccgc gctggtccag gccggcggag agagaaacgg aacgaccgag
     3721 atcaggtcgc ccgatacgcc ggaatcgatc aaccccttga ggttcggcag ctcgcccgcc
     3781 gccatccagg ggcgcaggag cttccaggtg gcgccgtcga gcccgatgac gagggccgcc
     3841 ggcttccggg acgggccgtt acaggcaggg gcaaaaacgc cgagcgccag cagggctgcg
     3901 agaaacccga tcgacgcgcg gcgcaagcgg cggaagctac cctaccggcc cgactgcttc
     3961 acgcccgtcc aggtcttgcc gacgacaggg tgtgccaggc cgcacgggtt cgaacccacc
     4021 acgctgccgt cggcgctgaa ctccacggtg ccgacgtacg gcttggtgtg gatgggggcc
     4081 ccgggaccgg ccacgatgga gaggatggag gtgcaatcgg ccacggcggc ctggttgtcg
     4141 gcgggacggg gaacgatctc gagccgatcg gcgctgtagg ccgtgaggta gaagtagccg
     4201 gccaccgcgg tgacaccggt acccgagccc gaaggctccg tggtctggca ggcgttctgc
     4261 ggatcccagg tgacgaggtt gccgccgttg ctcacgttgt accagtcgcg ccgtccgccc
     4321 gacgtggtgg gaaattcgag cgtgccgcac cgggtccact cgctgatgtc cacgccactg
     4381 cgcggtgtgc gattgaactt gacgccgaac gtgacaccgg cgatccccgc atcgcgcttg
     4441 ccgtccacca cgcagacgaa gccgtagtag tactcgttga cctttccgtt ggtgaccatc
     4501 tggtcgcacg gggcctgtgc cttgttgctc gagcaggcac gcccgctcat gctcgtgacc
     4561 ggtaccacgt ggatgatgat cttggcttcc tcgttcggcg tgccgtactg ggccagtgcc
     4621 ggccccgcgg acagcagggc cccggaaagt ccgatcagga ggggcagagc gggaaggatc
     4681 gaggggcgaa aggcggggcg ggaatcgggg cgctcaacga tgccgataca gcgcttcccg
     4741 ggggaaatca ggaacattcg ggcctatcct ccgtcagtgg cgacgaacaa gtatagaggt
     4801 cattcggccc cggtggaacc gccgaatggg gacgaacccg cagcgcgccc ctcaccccgt
     4861 tatcggcggg agggttcccg taaagcggcc caaaatcagg gttttcggcc ccaacaccga
     4921 cccccgcctg cttacggccc actggtcccg ggacagctgg ccgccccggc caggaagggc
     4981 gtgacgatca ccgcatcggc cgcgcccgtg gcgctgtcgc aattgaaatc cgagcaccag
     5041 tcatacgaat tgttcccgag cgggcccgtg tgctcgactg cgtccgtgat ccccgcccca
     5101 cacgtccccg ccgggttcca gcgcggactg tcggtggccc ggcgccccgc gttgtccacg
     5161 gcgtccgagg agaccacgcc gaattcctga agtcggatgc catcggcgaa gacctccccg
     5221 gcgtagtcca ggacacccgg tatggcggcc agcccgtact cgatgcaccc cccgccgccg
     5281 atcacgaaac gcgcctgccc ctggacattc gtgctctggg agaagacgtg cggcctggga
     5341 cccggcgtgt cgttgcacca gcaggccagc gtatcgcccg gcgtgagcaa tcgaatctgg
     5401 atattggcac tggcgatcgg ccccccgttg ccgacgatcg tcacgttgta cttggccccg
     5461 tggttcggaa gggcattggc gatcagcgtg gagggatgaa tctggaggca gcgcggcacc
     5521 gtgcagagtt cggggaggag gacgttgccg gcctcgttgg agcgcggctg aggctccgtc
     5581 ccaccggcgg ccagggccag atcgggcatg aacagcagca gggcgaaggc cgcgaacgcg
     5641 gcaagggaga cgaccagaga cccgattcgc cggagaggcc ggccgatcct catcacaaca
     5701 cctccaggct caggtggatg cgggatcccg tcgggatacc accttggagt gtagtggcat
     5761 tttgaatcac cggccccccc atcgcccgaa tcagtcggtc gccagcgcct tcacctccga
     5821 ccagctggcc gcctcgacag ggagcccgca aagtcgaccg cagggattga ctcccgtccg
     5881 gccgaagccg acggcgccca ggaaggtcga ttcatcggtt tcgggtcggt aacagacctg
     5941 gatgatgtcc tcggccgccc cgcagttggc caccttggca aggttatcca ccgggcgccg
     6001 cgtcactcgc atgatgtccg gcgtgtaggc cgccatgtag aagtaccccg ccactccgac
     6061 ggcgccgagg gccgcgttgc cggaggtctg gcagcgcgtg gtggcgtccc aggtgatgag
     6121 attgccaccg ccatcctgcg gccagttagt ggaggaaaat tccagcgagg cgcagagcgt
     6181 ccagccgtag acgtcgacac cctgaccgaa agccgagttg tagtcgatgc cgcactgcac
     6241 acccgcgatc ccttcgacct ggctcccatc cacgacgaga aggtaggtgt agtgggtggt
     6301 gggatagagc ggaagattgt tgataccggc attgaacccg acacagttgg ccggcaggtt
     6361 ggccgccctc acgcaggggt tcttgctcgt cgtcgaaagc gcatgcagca tgatcttggc
     6421 gtcgcggttc cccgcggcct cggcgggacg agtcatcagg agggctgacg acaggccaaa
     6481 cagggcggtg gcaaggaacg tgatagcggc tttcgtcatg aaatcacctc cggatcaacg
     6541 gcattggcag aaacaagaac ggggtggcgc tcgcacgcca ccccgccctc atcgagaccg
     6601 aaaatctagt tcgtgctggt gagcgtcttg acggcggacc agctcgtggg ctccacgggg
     6661 agcacgagtt cgcggccgca gggattgatc cccgtgttgc cggggccgaa gccgacggcg
     6721 ccgaggaacg tgtgggcgtc gtcaccggcc gggctgaaga cgatgtcttc gaccgaaccg
     6781 caattggcga ccttggccag ggcgtcgacc gggcgcgggg tgacgcgcat cacgtcgggc
     6841 gtgtaggcgc ccatgtagaa gaagcccgcc acggccacgg cgccgatggt ggcgttgccc
     6901 gcggtctggc agcgggtggt ggcatcccac gtgatcaggt tgccgccgcc ggcattgggc
     6961 cagccggtcg aggcgaactc gagcgtggcg cagagcgacc aggtgaagac gtcgaccccg
     7021 acgaactcgg cgccgttgta gttgatgccg cactggacgc cggcaatgcc ttcgacctgg
     7081 ctgccgttca ccacgagcag gtagacgtag tggaaggcag gggtgccgct ggtgagagtc
     7141 tggttgctga cgccgttgtc gtagccaccg caggcggcgg ggacgttggt ggcgcgcgta
     7201 caggggttct tggtggtggt ggtcagcgag tgcagcatga tcttggcgct cgcgttgccg
     7261 gccgcgtggg cgccggtggc aatcgccagc gtggcgactc cggcgagaac caccgatcgt
     7321 aggatacggg acatcgggtt ggcctcctta gggcagttcg gggggacgcg gcctggctgg
     7381 tacatcgtgc accggggacg ctcccggggg tcttgcaaaa aatggggggt ggccctgaag
     7441 ccacccccca aggtcctgcc gaaacgggat tagtggagca gcgacttcac gcccgaccag
     7501 gtggcatcca ccgtcgggac cggacgctca cgaccgcagg gattgatacc cgacgactgg
     7561 ccgaaaccga cggcacccag gaaggtgtcg ccaggggtgg ccacgtcgcc cggggcaccg
     7621 ccccagggga tgtcttccac cgagccgcag ttcgcaacct tggcgaaacc gtcgaccggg
     7681 cggggcgtga cgcgcatcac gtcgttgccg taggcgccca tgtagaagta gccggccacc
     7741 gcgacggtgc cgatgagggc gttacccgag gtctggcaac gggtggtggc atcccaggtg
     7801 atcaggttgc cgccgccgtc ctgcggcca
//
LOCUS       DASVOD010000682         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_774484, whole genome shotgun sequence.
ACCESSION   DASVOD010000682 DASVOD010000000
VERSION     DASVOD010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774484"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /gene="glgD"
                     /locus_tag="VF720_14620"
     CDS             complement(<1..1172)
                     /gene="glgD"
                     /locus_tag="VF720_14620"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEX7880646.1"
                     /translation="MMALVLAGGSTRDLSVLTDMRAASSLPFGGKYRVIDFTLSNLCN
                     SQIYQLGLLTQHAPLSLHAHVGIGKPWDLDRREGGVMILQPYQRQSDTRWYLGTADAV
                     RQNLDVLRHARARWALILPGDLVYKMDYSWLLEEHIERNAAVTLAVGPIDPKDAHRFG
                     VCQVDEHNHIVRFREKPTEAMEGLGFMGIACFNVDVLQKELCDHPDANNLVTEIIQPM
                     IERGERVDAYRYEGYWDDVGTVDAYYKASMDLLNDTPRLNLYDPEWVVYTRSVELPPA
                     RLGAQAMVERSLLANGCNVEGTVENSLLFPGVRVEEGAVVRNSIVMNGTSVAAGATLD
                     HVIVDKDVRIGVNVLIGHGEAAPHKDHPELLTGGLSIVGRDAVIPDGTIIGRHCVI"
     gene            complement(1227..>1902)
                     /locus_tag="VF720_14625"
     CDS             complement(1227..>1902)
                     /locus_tag="VF720_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880647.1"
                     /translation="IRLGVDDIKVTDDALIEEAGGIVRVDPSKVRLESAYDLGVFFNY
                     AHGLGSDWSLGGNVKMVRQSLVGDGSSFGIGADLGVLWQPSAGASFGLRLADITTTQI
                     KWDTGRHETVAPTVTLGGQGTTDINALRGTLTGAADVQLAFEDLGDADQFSSGSMSGN
                     LHAGLEYWYNHVVALRAGTNSGHFAAGAGVRFKLGPMQHFGVDYAFLDHDELDATNRV
                     TLNLGW"
BASE COUNT          306 a          605 c          667 g          324 t
ORIGIN      
        1 tcgatgacgc aatgccggcc gatgatcgtg ccgtcgggaa tgacggcgtc gcggccgacg
       61 atcgagagtc cgcccgtgag aagctcggga tggtccttgt ggggcgccgc ctcgccgtgg
      121 ccgatgagga cgttgacgcc gatccgcacg tccttgtcca cgatgacatg gtcgagggtg
      181 gcgccggcgg ccaccgaggt gccgttcatg acgatggagt tgcggaccac cgccccctct
      241 tccacgcgga ctcccgggaa gaggagcgag ttttcgacgg tcccctccac gttgcagccg
      301 ttggccagga ggctccgttc caccatggcc tgcgcgccga ggcgggcggg cggcagttcc
      361 acgctgcggg tgtacacgac ccactccggg tcgtagaggt tcaggcgcgg cgtgtcgttc
      421 aggaggtcca tggaagcctt gtagtaggcg tccacggtgc cgacgtcgtc ccagtacccc
      481 tcgtaacggt aggcatccac ccgctcgccc cgttcgatca tcggctggat gatctccgtg
      541 acgaggttgt tggcgtccgg gtggtcgcac aactccttct ggaggacatc cacgttgaag
      601 caggcgatcc ccatgaaacc gaggccctcc atggcctcgg tgggcttctc gcggaacctc
      661 acgatgtggt tgtgctcgtc cacctggcag acgccgaagc ggtgggcgtc cttcgggtcg
      721 atcgggccca cggccagtgt caccgccgcg ttccgctcga tgtgctcctc gaggagccag
      781 gagtaatcca tcttgtagac gaggtcgccg gggaggatga gcgcccagcg cgcccgcgcg
      841 tggcgaagga catcgaggtt ctgccgcacc gcgtcggcgg tgccgaggta ccagcgggtg
      901 tcgctctggc gctggtaagg ctgaaggatc atcacgcccc cctcgcgccg atcgaggtcc
      961 cacggcttgc cgatgccgac gtgggcatgg agcgaaaggg gcgcgtgctg cgtgaggagg
     1021 ccgagctggt agatctgcga gttgcagagg ttcgacaggg tgaagtcgat cacccggtac
     1081 ttgccgccga acggaaggga gctggcggcg cgcatgtcgg tcaggacgga tagatcacgg
     1141 gtactgccgc cggccagcac cagcgccatc actcgccgca accgaagcct tcccttctgt
     1201 cggccccggc tcggggcacc gcgagcctac cagccgaggt tcagcgtgac ccggttggtg
     1261 gcgtccagtt cgtcgtgatc gaggaacgcg tagtcgacgc cgaagtgctg catcggtccg
     1321 agcttgaagc ggacaccggc gcctgccgcg aagtggccgg aattggtgcc ggcccgcagt
     1381 gcgacgacat ggttatacca gtattcgagt cccgcatgaa ggttgccgga catcgagccg
     1441 gaggagaact ggtcggcgtc acccaggtct tcgaaggcca gctgcacgtc ggccgcgccg
     1501 gtgagcgtgc cgcggagtgc gttgatgtca gtcgttccct gtccgccgag ggtaaccgtg
     1561 ggggccaccg tctcgtggcg gccggtatcc cacttgatct gcgtggtggt gatgtcggcc
     1621 aatcggaggc cgaacgaggc cccggccgac ggctgccaca gcacgcccag gtcggcgccg
     1681 atgccgaagg aggagccatc ccccaccagg ctctgccgga ccatcttgac gttgccgccg
     1741 agcgaccagt cggagccgag accgtgggcg tagttgaaga agacacccag gtcgtaggca
     1801 ctctcgaggc ggaccttgga cgggtcgacc cggacgatgc caccggcttc ctcgatcagc
     1861 gcgtcatcgg tcaccttgat gtcgtcgacg ccgaggcgga tg
//
LOCUS       DASVOD010000683         8777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_356855, whole genome shotgun sequence.
ACCESSION   DASVOD010000683 DASVOD010000000
VERSION     DASVOD010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8777
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356855"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="VF720_14630"
     CDS             <1..956
                     /locus_tag="VF720_14630"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX7880648.1"
                     /translation="LDEANHARRVAQHLKVEHHLIELKEVGVDELRRSVEVVEDLVAD
                     PALMPFIGVSRRARENLTVALVGEGSDETNVGYKGFLWMKKMLARRRLTRLIPLLPKT
                     GDLATKLGHPALGEIDFLARYTNVGIPVDDYPPFRPGLPSAVERIREEIHRLTDRAHG
                     LSTLGRNRLFRIEAWMKDDLLIKVDKTTMAASIEARVPFLDHTYVEWSFGLPEEMVLR
                     KGETKAVLRALARKLLPPEIAGRRQHGLVVPMHDVLGSIGHDALQPILRHPEALWRRI
                     FPEKPIVALFDRHRGGDTSLGFFVYQVVNAEFWRERWLTPR"
     gene            1055..1969
                     /locus_tag="VF720_14635"
     CDS             1055..1969
                     /locus_tag="VF720_14635"
                     /inference="COORDINATES: protein motif:HMM:NF042644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6588 family protein"
                     /protein_id="HEX7880649.1"
                     /translation="MHRTFPFRSALILGLVLASVPPSPAGADGQLEDNLSAYVGVNAE
                     GYLGPLRDGIGGALNSGLFMYPSPPEFGFHLRLDGRAGVTMYKDEDRTFMATTDPSFG
                     DPVTVEAPTVVGSTESYTITDPETGAVYTFPGGFDVDSFGLAAPQLTVGVIYGTEFMG
                     RFITYDPNDLEIDNVQLWGVGARHSLSQWLSEPPVDIAIHGMYQNLQVDDDFIDASTL
                     SLGAEVGKTFGIFSGYVGAAYHTVDLDATYESSASGTTETVRVTLERSNTFDLGLGAT
                     LRLAFLHLNGEYHFAERNSYALGIGLGN"
     gene            1980..2594
                     /locus_tag="VF720_14640"
     CDS             1980..2594
                     /locus_tag="VF720_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880650.1"
                     /translation="MTNRKPLIALGLGLLFLVLKADSCIIEDRMVDAVVTQTIPYVWH
                     TEGDNAAGSDSAVVSLAADVEAALDEFEGDVDVTAIHISGGTYRVLENRGFVGAHMGT
                     VTIQAVGDEPLTILTYDVPNPPGNADGASGGTGVDVSLVAAGINLLNERLAEYLDTRD
                     PSRLQFTFRTEWNSTAPSTDYDFDWETALILQVVGTIEVQVPNP"
     gene            complement(2603..3955)
                     /locus_tag="VF720_14645"
     CDS             complement(2603..3955)
                     /locus_tag="VF720_14645"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7880651.1"
                     /translation="MSSPRLVVLGGGREVGANSFLLELDGARLLLDAGSHPRHEGAES
                     LPRFELARDPDAILITHAHLDHVGGLPSAVARWPRGRIYWSRATEMLSVRQLHAAVAV
                     MRKEQEAGRLAGPLLFGHDDVETLGWRGWPLMDGQPTRMPTNARVMVTPFASGHVLGS
                     QGFVIESASARVVYTGDVCGHARELQPGMAPPAVKADILILEGTYGGTPEYSGDRYED
                     EVTRFASGVERVLRRGGSVLVPAFALGRTQEIVAMLRRLMGQGKLPRVPVRTSGLGRA
                     MSEVHDRCVDEPSMRPRKWRLARWPRVLEAADLLDLRRILEKPSIHVLTSGMMTAGTS
                     SALAAEAMIGDERHGIFFVGWTDPAELGHRVQHAPLGEWIEFRRGRPMTQRRTDAVDR
                     FWFSGHADRRRLLQVVDHFDPEHVVLVHGEPAALEWLAENASPRRRVHRPATGETLTF
                     "
     gene            complement(3998..4444)
                     /locus_tag="VF720_14650"
     CDS             complement(3998..4444)
                     /locus_tag="VF720_14650"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX7880652.1"
                     /translation="MMRWAHRQAALVELELLLFSVGGVRVGTPLERVAGVLTDVSLLK
                     DNRDVGAIPYNGENVPLVRAENVFEVGARTDSAPGAVILFRTLRGLCAVAVDEALDVL
                     RVTPGDRLYRFPPEESTWSSTRRPWGFVELGDVPILLVDFGPLRVH"
     gene            complement(4452..4784)
                     /locus_tag="VF720_14655"
     CDS             complement(4452..4784)
                     /locus_tag="VF720_14655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7880653.1"
                     /translation="MELHSESLDGWTRIRLVGNLDAGSAGETQRQILPLVDDGARRYL
                     FELDDVPVIDSAGLAVLVKVYREIKGKGGRMALSGVQREPMRVFHLTRLDRIFPIHPT
                     AESARAAA"
     gene            complement(4887..6101)
                     /locus_tag="VF720_14660"
     CDS             complement(4887..6101)
                     /locus_tag="VF720_14660"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX7880654.1"
                     /translation="MIDSFVRFRPMPACLLVLMMAMAALWGGGPARAAEPPRVTRIRQ
                     SSETGTTRVVVELSQPAPWKQMALDGPRRVVLDIEAGADPSVTASGVGDGRLHRIRVG
                     TQGESALRIVFDVDAIRDYRAFELPADADQPARLVFDFFGGEPSNGAAVAPSAPDDRK
                     TPAVPAIVSAPARTRPITVVIDAGHGGQDPGARGHGNEEKDICLAIARKVAAEIEKRP
                     GFRARLTRDDDRFIPLRGRIEVAEKHDADAFVSIHCNAAKASSATGTEVYFLSLSGAT
                     DEASRELARLENAADLMGGVAPEADDDLTSILFDMQQADNLQRGSILAASVMESLGGH
                     SDLSTRGVKQAGFVVLKSPRIPSILVETAFITNSRESRMLASGTFQVAFARRVAEGVG
                     RFFESTALAGLR"
     gene            complement(6098..7663)
                     /locus_tag="VF720_14665"
     CDS             complement(6098..7663)
                     /locus_tag="VF720_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX7880655.1"
                     /translation="MTTWYEAGPDAGPPLKHGRRPMRTPLLAGLALMTGIAVGVTLSG
                     AFDWTPPGFATAPARPAPTAPPADDDQLPEIPPSPFVTVARRVVPAVVYVDADRRAAA
                     AEAANDRTAPDFMRRLFPDDERDEANLPSSGSGFIVDKDGHILTNNHVVAGADQITVH
                     LGDGRSYRGEIIGTDDKTDVAVLRIRPPSGDPPLPVVRLGDSEKVEVGEWAMAIGNPL
                     GQLEGSVTVGVISAKGRADLDIMGGGPDYQDFLQTDASINFGNSGGPLVNARGEVIGI
                     NTAINPTGQGLGFAIPINMARLVSDQLIADGRVTRAYMGIVPQALSGELAEGLGIAIR
                     RGILVAHVEVGGPAEVAGLRTKDVITEFDGQPVEEVNRFRRFVAEATVGHRARLTVVR
                     DGRSMVKNVELVRRPDDQPEERPDPRRTDPLPTEWLGARFEPMSRDLADDWQIEYEPG
                     LAVTDVAPGSPADEAGLGEGDVVREVNDRPVRSLEEWKGAMESAMGRTRPLVLLVSRG
                     GVVSYVAIRMGDR"
     gene            7780..8565
                     /locus_tag="VF720_14670"
     CDS             7780..8565
                     /locus_tag="VF720_14670"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEX7880656.1"
                     /translation="MELRAHPARRGGPVYFVGDAHIGTAGPTEEAEKEERLLKLLDEI
                     DAQGAALVVMGDLFDFWFEYGHAIPSRGFNVLARLKRLVDSGLPIDFLGGNHDWWAGP
                     FLQSRIGIHAHSEPIEVECQGRKLFLAHGDGLAPGDNGYRVLRKVFRNPLAIAAYRWI
                     HPDLGIPLATRSSHTSRRHTETQPVIGARLWETIARPQFDRGRDAVLIGHFHRPIHVR
                     SGNRDFIINGDWMGHWSYSVLEAGELTLRIWPEGKVVIPEQIT"
BASE COUNT         1432 a         3099 c         2857 g         1389 t
ORIGIN      
        1 atctcgacga ggccaaccat gcccggcgcg tcgcgcagca cctcaaggtg gaacaccatc
       61 tcatcgagct gaaggaagtc ggcgtggacg agctgcgccg ctcggtcgag gtggtcgagg
      121 acctggtggc cgatccggcc ctgatgccgt tcatcggcgt ctcgcggcgc gcgcgagaga
      181 atttgaccgt ggcgctggtc ggcgagggga gcgatgagac caacgtcggc tacaagggct
      241 tcctctggat gaagaagatg ctggcccgtc gccgcctcac ccgcctcatc cccctgctgc
      301 cgaaaacggg ggatctggcc acgaagctcg gccacccggc cctcggcgag atcgacttcc
      361 tggcccgcta caccaacgtc ggcatcccgg tggacgacta cccaccgttc cggccgggcc
      421 tcccctccgc ggtcgagcgc atccgcgaag agatccaccg cctcaccgac cgcgcccacg
      481 ggctttccac cctcggccgc aatcgcctct tccgcatcga ggcgtggatg aaggacgacc
      541 tcctcatcaa ggtggacaag accacgatgg ccgcctccat cgaggcccgt gtcccgttcc
      601 tggatcatac gtacgtggag tggagcttcg gcctgccgga ggagatggtc ctccggaaag
      661 gcgaaacgaa ggcggtcctc cgcgcgctgg cccgaaaact cctgcctccc gagatcgcgg
      721 ggcgccgcca gcacggcctg gtcgtcccga tgcacgacgt gctcggctcg atcggacatg
      781 acgccctgca gccgatcctt cggcatccgg aggcgctctg gcggcggatc tttcccgaaa
      841 agccgatcgt ggcgctgttc gatcgtcacc gcgggggtga caccagcctg ggcttcttcg
      901 tctaccaggt cgtcaacgcg gaattctggc gcgaacggtg gcttaccccc cgttaacgcg
      961 ccccgacaag tctgctagct taccgactgg cggcgcatcc cggcgaacga ccggggatgg
     1021 caatgcgtca cgccgccctc ccacggaggt gttgatgcat cgaacgtttc cattccgctc
     1081 ggcgctgatc ctcggtctcg tcctggcttc cgtccccccc tcgccggccg gggcggacgg
     1141 acagctcgag gacaacctct ccgcgtacgt cggcgtcaat gccgagggat acctggggcc
     1201 gctgcgtgac ggcatcgggg gggcgctgaa ctccgggctg ttcatgtacc cgagccctcc
     1261 cgagttcggg tttcatctcc gactcgacgg ccgggcgggc gtcaccatgt acaaggatga
     1321 agacaggacc ttcatggcca cgaccgatcc ctccttcggc gatcccgtta ccgtggaggc
     1381 cccgacggtg gtcggctcga ccgaaagcta cacgatcacc gatccggaaa cgggagcggt
     1441 ctacaccttt cccggcggat tcgatgtgga cagcttcggc ctggcggcgc cccagctcac
     1501 cgtgggcgtg atctacggca ccgagttcat ggggcggttc atcacgtacg atcccaacga
     1561 cctcgagatc gacaatgtcc agctctgggg tgtcggcgcc cgacattccc tgagccagtg
     1621 gctcagcgag ccgcccgtcg acatcgccat ccacggcatg taccagaacc tccaggtcga
     1681 cgacgacttc atcgatgcca gcacgctcag cctcggcgcc gaggtgggca agaccttcgg
     1741 catcttctcc ggctatgtcg gcgcggcgta tcacacggtc gacctggacg ccacgtacga
     1801 gtcgagcgcc tccggcacca cggagacggt ccgtgtcacg ctcgagcgga gcaacacctt
     1861 cgacctgggg ctgggggcca ccctcaggct cgccttcctc cacctgaacg gcgaatacca
     1921 cttcgccgaa cgcaacagct acgcccttgg catcggcctc ggcaactagg gagggagaga
     1981 tgacgaatcg aaagccattg atcgcgctcg gcctcggact cctgttcctg gtcctcaagg
     2041 ccgacagctg catcatcgaa gaccgcatgg tcgacgccgt ggtcacccag acgatcccgt
     2101 acgtgtggca taccgagggg gacaacgcgg ccggcagcga ttccgccgtc gtcagcctgg
     2161 ccgccgacgt tgaggcggcg ctcgacgagt tcgagggtga tgtcgacgtc accgcaatcc
     2221 acatctcggg cggcacctac cgcgttctcg agaaccgcgg cttcgtgggg gcccacatgg
     2281 ggacggtcac catccaggcc gtgggcgacg aaccgctcac catccttacc tacgacgtgc
     2341 cgaatcctcc cggcaacgcc gacggcgctt cgggaggcac gggcgtcgac gtcagcctcg
     2401 tggcggccgg catcaacctc ctcaacgagc ggctcgccga gtacctcgac acgcgcgatc
     2461 cgtcccggct ccagttcacg ttccggacgg aatggaattc caccgcgccg tcgaccgact
     2521 acgacttcga ctgggagacg gccctcatcc tccaggtcgt cggcacgatc gaggtgcagg
     2581 tgccgaaccc gtagggccga cctcagaacg tcaacgtctc cccggtggcc gggcgatgca
     2641 cccgccgccg gggagaagca ttttcggcca gccactcgag ggcggccggc tcgccgtgca
     2701 ccagcaccac atgttccggg tcgaagtggt cgacgacctg cagcaggcgc cggcggtcgg
     2761 catgcccgga aaaccagaag cggtccaccg cgtcggtccg ccgctgcgtc atcgggcgcc
     2821 cccgtcggaa ctcgatccac tcgcccaggg gcgcatgctg tacccggtga cccagctcgg
     2881 cagggtcggt ccagcccacg aagaagatcc cgtgccgctc atcgccgatc atcgcctcgg
     2941 cggccagggc ggatgaggtg ccggccgtca tcatccccga cgtgaggacg tggatggacg
     3001 gcttttccag gatgcggcgg aggtcaagca ggtcggcagc ctcgagcacg cgcggccagc
     3061 gggccagccg ccatttccgc ggccgcatcg agggttcgtc gacgcaccgg tcgtgcacct
     3121 cgctcatggc ccgtcccagg cccgacgtgc gtaccggcac gcgcggaagc ttcccttgcc
     3181 ccatcagccg gcgaagcatg gccacgattt cctgcgtgcg gccgagggca aatgccggca
     3241 cgaggaccga acccccgcgc cggagcaccc gctccacgcc ggaggcaaag cgggtcacct
     3301 cgtcctcgta acgatccccc gagtattcgg gggtgccccc gtaggttccc tcgaggatga
     3361 ggatgtcggc cttgacggcg gggggcgcca tgcccggctg cagctcgcgc gcgtgaccgc
     3421 acacatcccc cgtgtacacc acgcgggccg aggccgactc gatgacgaac ccctggctgc
     3481 ccagcacatg gccactggcg aaaggcgtca ccatcacgcg cgcgttggtg ggcatccggg
     3541 tcggttgacc atccatcagg ggccagccac gccaaccgag ggtttccacg tcgtcgtggc
     3601 cgaagagaag aggcccagcc agccggccgg cctcctgttc cttccgcatc acagccaccg
     3661 cggcgtgcaa ttgccggacc gacagcatct cggtggcccg gctccagtag atgcgccccc
     3721 ggggccagcg cgccaccgcc gagggaaggc cgccgacatg gtccaggtgg gcgtgcgtga
     3781 tgaggatggc gtcgggatcc cgggccagtt cgaaacgggg aaggctttcg gcaccctcat
     3841 ggcgaggatg cgagccggcg tccaggagga gccgcgcgcc gtccagttcg aggaggaagg
     3901 aattggcgcc gacctcgcgg ccgccgccca ggaccacgag gcggggggaa ctcatgccgc
     3961 catggtagcc gttccggccg acatggcgcg gaaccggcta gtgaacccgg agcggcccga
     4021 agtcgaccag cagaatcgga acgtcgccga gctccacgaa tccccaggga cgtcgcgtcg
     4081 acgaccaggt cgactcttcc ggcggaaacc ggtagaggcg atcacccggc gtcacacgga
     4141 ggacgtcgag cgcctcgtcc accgccacgg cacagaggcc acggagagtc cggaagagga
     4201 tgacggctcc cggcgccgag tcggtgcggg caccgacctc gaagacgttc tcggcgcgca
     4261 ccagcggcac gttctccccg ttgtacggga tggcgccgac gtcgcggttg tccttgagga
     4321 gcgaaacgtc ggtcaggaca ccggccaccc gctcgagcgg ggttccgacc cggacgcccc
     4381 ccacggaaaa gaggaggagt tccagctcga cgagagcggc ctggcggtgg gcccagcgca
     4441 tcatgagggt atcaggccgc cgcccgcgcc gattcggcgg tcgggtggat cgggaagatg
     4501 cggtccagcc gggtaaggtg gaacacgcgc atcggctcgc gctgcacgcc ggagagcgcc
     4561 atgcgccccc ctttcccctt gatctcgcga tagaccttca cgaggaccgc gaggccggcg
     4621 ctgtcgatca ccggcacatc gtccagctcg aacaggtagc gacgcgcccc gtcatcgacg
     4681 agaggcagga tctggcgctg ggtttcgccg gctgagccgg cgtcgaggtt gccgacgagg
     4741 cggatccggg tccagccgtc cagggattcg gagtgaagtt ccatgacccg ggtatcggcc
     4801 ggcccgggcg ttccccttag ccggcaggta tcaggatctc ccgggggcgc gccatcccgc
     4861 ccgtcggggc ggtcagcgca gcactgtcag cgaagcccgg ccagcgcggt actctcgaag
     4921 aatcgcccga ccccttcggc cacgcggcgc gcaaaggcca cctggaacgt cccggacgcc
     4981 agcatgcgtg attccctgct gttcgtgatg aaggcggttt cgacgaggat ggaggggatc
     5041 cggggcgatt tcagcaccac gaatccggcc tgcttcaccc cccgggtgga gaggtcggaa
     5101 tgtcccccca gcgattccat gaccgaggcg gccaggatcg agccgcgctg caggttgtcg
     5161 gcctgctgca tgtcaaacag aatggaggtc aggtcgtcgt cggcctcggg cgccacgccg
     5221 cccatcaggt cggcggcatt ctccagccgg gccagttcgc gcgaggcttc gtcggtggcg
     5281 ccggagaggg agaggaagta aacctcggtg ccggtggccg agctcgcctt ggcggcattg
     5341 cagtggatgc tcacgaaggc gtccgcgtcg tgcttctcgg ccacctcgat ccggccgcgc
     5401 agtgggatga agcgatcgtc gtcgcgcgtc agccgggcgc ggaatccggg gcgcttctcg
     5461 atttcggccg ccaccttccg cgcgatggcc aggcagatat ccttctcctc gttgccgtgg
     5521 ccgcgggcgc ccgggtcctg gccgccgtgc ccggcatcga tcaccacggt gatcggacgg
     5581 gtccgggccg gcgccgacac gatcgccgga accgcgggtg ttttccggtc atcgggggcg
     5641 gacggggcca ccgccgctcc attcgatggc tcgccaccga agaagtcgaa taccaggcgc
     5701 gccggctgat cagcatcggc gggcaattcg aacgcgcgat agtcgcggat ggcgtcgacg
     5761 tcgaaaacga tgcgcagtgc cgactctccc tgcgtcccca cgcggatccg atggaggcgc
     5821 ccgtccccca ccccgcttgc cgtgaccgag gggtcggccc cggcctcgat gtcaaggacg
     5881 acgcgtcgtg gaccgtcgag cgccatctgc ttccagggtg cgggctggga aagttcgacc
     5941 accacgcggg tggtcccggt ctcgcttgac tggcggatcc gggtcacgcg ggggggttca
     6001 gctgcccggg cggggccacc tccccagagc gctgccattg ccatcatcaa caccagaagg
     6061 caggccggca tcggccggaa acgaacgaac gaatcgatca tcggtctccc atccggatgg
     6121 ccacgtaact caccacccct ccccggctga ccagcagcac cagcgggcgc gtcctcccca
     6181 tggccgactc catcgcaccc ttccactcct cgagcgatcg gacggggcgg tcattcacct
     6241 cgcggaccac gtccccttcg ccgagtcccg cctcgtcggc cggacttccc ggcgccacgt
     6301 cggtgaccgc gaggcccggc tcgtactcga tctgccagtc gtcggccagg tcacggctca
     6361 tcggctcgaa gcgcgcgccc agccactcgg tgggcagcgg gtcggtccgc cgtggatcgg
     6421 ggcgttcctc cggttgatcg tccggccgcc ggaccagctc cacgtttttc accatgctcc
     6481 ggccgtcccg gactaccgtg aggcgggccc ggtgccccac cgtggcctcg gccacgaagc
     6541 ggcggaagcg gttcacttcc tccactggct ggccgtcgaa ctcggtgatc acgtccttgg
     6601 tgcgcaaccc ggctacctcg gccggcccgc ccacctccac gtgggccacc aggattccgc
     6661 gccggatggc gattcccagc ccctcggcca gttccccgga cagcgcctgg ggcacgattc
     6721 ccatgtaggc ccgggtcacg cggccgtcgg cgatgagctg gtcactgacc aatcgcgcca
     6781 tgttgatcgg gatggcaaag cccaacccct ggccggtcgg attgatggcg gtgttgattc
     6841 cgatcacttc gccgcgggca ttgaccaacg gtccgcccga gttgccgaag ttgatcgacg
     6901 cgtcggtctg caggaagtcc tggtagtcgg gcccccctcc catgatgtcg aggtcggccc
     6961 gtcccttggc gctgatcacg cccacggtca ccgagccttc gagttgcccc agcggattcc
     7021 cgatggccat ggcccactct cccacctcca ccttttcgga atcgccgagg cgcaccacgg
     7081 ggagcggcgg atctcccgac ggtggacgga tgcgcagcac cgccacgtcg gtcttgtcgt
     7141 ccgtcccgat gatctcgcca cggtagctcc tgccgtctcc caggtggacg gtgatctggt
     7201 cggccccggc caccacgtgg ttgttggtca ggatgtgccc gtccttgtcg acgatgaatc
     7261 ccgacccgct gctcggcagg ttggcctcgt cgcgctcgtc gtcgggaaag agacgtcgca
     7321 tgaaatcggg cgccgtgcga tcgttcgccg cctcggctgc ggccgcccgt cgatccgcgt
     7381 cgacgtagac gacggccggc accacgcggc gtgccaccgt cacgaacgga ctcggcggaa
     7441 tttcagggag ctggtcgtcg tcggccggcg gcgcggtcgg cgccgggcgg gccggggcgg
     7501 tggcgaaacc cgggggagtc cagtcgaacg caccggacag agtcacgccg accgcaattc
     7561 ccgtcatcag tgcaagcccc gccagaaggg gcgtgcgcat ggggcggcgg ccgtgtttca
     7621 acggcggacc ggcatccggt cccgcctcat accatgtcgt catcgtgctc ctcgcccccc
     7681 ggccgggaca tgataggcgc cgggggccgc ctcgtgaagc caccttgtgc cgatcggccg
     7741 ggaaatccgg ccccccggcg cttctggtat aaaccgccga tggaattgcg cgcgcacccc
     7801 gccaggcggg gcggccccgt ttatttcgtc ggagatgccc atatcggcac cgcggggccg
     7861 accgaggagg ccgagaagga ggagcgcctc ctgaagctcc tggacgagat agacgcacag
     7921 ggggccgcac tcgtcgtgat gggtgatctt ttcgacttct ggttcgaata cggccacgcc
     7981 atcccgtcgc gcggcttcaa cgtcctggcc cgcctgaaaa ggctcgtcga ctcgggtctg
     8041 ccgatcgatt tcctcggcgg caatcatgac tggtgggccg gccccttcct tcaatcccgg
     8101 atcggcattc acgcccactc cgagccgatc gaggtcgaat gccaggggcg caaacttttc
     8161 ctggcccacg gcgatggcct cgctcccggc gacaacggct atcgggtgct ccggaaagtc
     8221 tttcgcaacc cgctcgccat cgcagcctat cgctggatcc accccgatct cggaattccc
     8281 ctggccaccc ggtcgagcca caccagccgc cgtcataccg aaacgcagcc ggtcatcggg
     8341 gcccggctct gggagacgat tgcccgtccg caattcgacc gcggccggga tgccgtattg
     8401 atcggccact tccaccgtcc gatccacgtt cgctcgggga accgggactt catcatcaat
     8461 ggcgactgga tgggtcactg gtcctattcg gtgctcgagg cgggggagct cacgctccgg
     8521 atctggcctg agggtaaggt cgtgatccct gaacaaataa cataatcctt atatatctat
     8581 ctgtttgatt cactgggcaa ctatctaagg acggccaact taccgatgaa ctccggccgg
     8641 gagtccacgg tccagaggta gattcccggg gccgtgaccc caccactccc ggacaatgtc
     8701 cacgcaaccg accctgagct gccggcgctg ccgctccata cttcccgacc cgcctggtca
     8761 cgaaccgcca cgcgggc
//
LOCUS       DASVOD010000684         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_408946, whole genome shotgun sequence.
ACCESSION   DASVOD010000684 DASVOD010000000
VERSION     DASVOD010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408946"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF720_14675"
     CDS             <1..324
                     /locus_tag="VF720_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX7880657.1"
                     /translation="HNDHNGLNLARESALEKGLAACDELCGFITAVTLVRPSKNLDEV
                     TAESVLKKMKDKAFARQVNREEIRHGAEAYGVELKDHITFLIDALKPSARDLGLDGSA
                     AAPVA"
     gene            355..1845
                     /locus_tag="VF720_14680"
     CDS             355..1845
                     /locus_tag="VF720_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX7880658.1"
                     /translation="MTRPATIGALRAAGYTDRTVKEELRTNLVRALKSGETLFPGIIG
                     YDRTVIPQLVNAILSKHDVILLGLRGQAKTRLLRSLTRFLDEYIPVVAGSEISDHPFH
                     PVSAYARRLVAERGDDTPIEWVHRDRRYHEKLATPDVTIADLIGDIDPIKAAAKKLDF
                     SSEEVIHYGLVPRANRGIFAINELPDLQARIQVGLLNVMQEKDVQIRGFDVRLPLDVA
                     IVYSANPEDYTNRGSIITPLKDRIDSQIMTHYPEDLAAAMAITDQEAWTERDGSARLV
                     VPEWLREVIEEIAFQARESDYVDQNSGVSARLSITALENVLSNMERRALKTGDDPVVA
                     RVADLQAAVPSLTGKIELVYEGEQEGPLKVARYLIGRAVKEIFESHFPPAHRGKKKSA
                     EREEVATSPYRKIVDWFSQGKKLEIGDDDTALAYFEALNQVPGLKDVAARHAGTDDPI
                     LVAPIMELILEGLHQSAILAKEELEGQVRFLDMFQTMLSNLGDQKR"
     gene            1853..2938
                     /locus_tag="VF720_14685"
     CDS             1853..2938
                     /locus_tag="VF720_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7880659.1"
                     /translation="MKFRYSEWDDALAEQLRQFETLRSLFNQLLLQTDGDVDEAFRWL
                     EYLKEKGILPPGFDLDAFKAELEKDDLIRQEGTRTVLTKKGERSIRQESLDLIFGQLR
                     KGAGGEHRTAHSGEGGENLPETRPYVFGDSISDLDQMLTIRSALRHGLDEIAITEDDL
                     HVHEKEHRTSVATVLCLDISHSMVLYGEDRITPAKRVALALSELILTRYPKDSLDILL
                     FGDDARVVPLEELLSSGVGPFHTNTKAALRLAQQILRTKKHVQKQVFMITDGKPSAIL
                     EEGRIYKNSFGLDPKIVNRTLDEAVACRRKNIAITTFMVAKDHALVEFVEEMTRLNKG
                     RAYYTGLDNLGGYVFADFVKNRRRVVR"
     gene            3128..3361
                     /locus_tag="VF720_14690"
     CDS             3128..3361
                     /locus_tag="VF720_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880660.1"
                     /translation="MNTCEVCGNTYDRTFEVIQEGKSHVFDSFECAIHALAPTCEHCG
                     CRVVGHGVQDEGAIFCCVHCAEESGVTGLRDRQ"
     gene            3376..3795
                     /locus_tag="VF720_14695"
     CDS             3376..3795
                     /locus_tag="VF720_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX7880661.1"
                     /translation="MTVREAMTSRTELATPDMTLRDAARLMRDCDIGSLPVGENDRLI
                     GMITDRDITIRAVAEGRDVHTTPVRDAMTTKVLYVYDDEPIEVAAQTMADLQVRRLPV
                     LDRERHLVGILALSDVSIEGPPTSAAEALRGVSEPSA"
BASE COUNT          783 a         1336 c         1200 g          576 t
ORIGIN      
        1 cacaacgacc acaatgggct gaatctcgcg cgcgaatcgg cgctcgaaaa ggggttggcc
       61 gcctgtgacg agctctgcgg gttcatcacg gccgtcacgc tcgtccgtcc ctcgaaaaac
      121 ctcgacgaag tgaccgccga gtcggtcctc aagaagatga aggacaaggc gttcgcacgg
      181 caggtcaacc gcgaggagat ccgccatggc gccgaagcct atggcgtgga actcaaggac
      241 cacatcacgt ttctgatcga tgcgctcaag ccgtccgccc gagacctcgg cctcgacggg
      301 tcggcagcgg cgcctgtcgc gtaagctttt ccgcctgtca ggagcccgtc accgatgacc
      361 cgccccgcca cgatcggcgc cctgcgcgcc gccggctata ccgaccgaac cgtgaaggaa
      421 gagctgcgga ccaatctcgt ccgcgcactg aaatcggggg aaactctctt ccccggcatc
      481 atcggctacg accgcacggt cattccgcag ctggtcaacg ccatcctgtc gaagcacgac
      541 gtcatcctgc tgggcctgcg cggccaggcc aagacgcgcc tgctgcgcag cctcacccgg
      601 ttcctcgacg agtacatccc ggtggtggcc ggcagcgaga tcagcgacca tccctttcac
      661 ccggtgtcgg cgtacgcccg acggctggtg gctgaaagag gagacgacac gccgatcgaa
      721 tgggtgcacc gcgaccggcg ttaccacgaa aagctggcca cgcccgacgt gaccattgcc
      781 gacctgatcg gagacatcga cccgatcaag gccgccgcca agaagctcga cttctcgagc
      841 gaggaggtca tccattacgg cctcgtgccg cgggccaacc gcggcatctt cgccatcaac
      901 gaactcccgg accttcaggc gcgcatccag gtggggctcc tgaacgtcat gcaggaaaag
      961 gacgtgcaga tccgcggctt cgacgtccgc ctgccgctcg acgttgccat cgtctactcg
     1021 gccaaccccg aggactacac caaccgcggc agcatcatca cgccgctcaa ggaccgcatc
     1081 gacagccaga tcatgacgca ctacccggag gacctggccg ccgccatggc catcaccgac
     1141 caggaggcct ggacggagcg cgacggctcc gcgcggctgg tggtgccgga gtggctgagg
     1201 gaagtcatcg aggaaatcgc cttccaggcg cgcgagagcg actacgtgga ccagaactcg
     1261 ggcgtgtcgg cacgcctgtc gatcaccgcg ctcgagaacg tcctctccaa catggaacgc
     1321 cgggcgctca agaccgggga cgacccggtc gtggcgcggg tggccgacct ccaggccgcg
     1381 gtcccctcgc tcaccgggaa gatcgagctg gtgtacgagg gagagcagga ggggccgctc
     1441 aaggtggccc gctacctgat cggccgcgcc gtgaaggaga tcttcgagag ccacttcccg
     1501 ccggcccacc gcggcaagaa gaagagcgcc gagcgcgagg aagtggccac gagcccgtac
     1561 cgcaagatcg tcgactggtt cagccagggg aagaaactgg agatcggcga cgacgacacg
     1621 gcgctcgcct acttcgaggc gctgaaccag gtgcccggcc tcaaggacgt ggccgccagg
     1681 catgcgggca ccgacgaccc gatcctggtg gcgcccatca tggagctgat cctcgaagga
     1741 ctgcaccaga gcgccatcct ggccaaggaa gagctcgagg ggcaggtccg cttcctcgac
     1801 atgttccaga cgatgctctc gaacctgggc gaccagaagc gctgagcccg tcatgaaatt
     1861 ccgctactcc gaatgggacg acgcgctggc ggagcagctt cgccagttcg agacgttgcg
     1921 ctccctcttc aaccagctcc tcctccagac cgacggcgac gtggacgagg cgttccgctg
     1981 gctggagtac ctgaaggaaa aggggatcct gccacccggc ttcgacctcg atgccttcaa
     2041 ggccgagctg gaaaaggacg acctgatccg ccaggaaggc acccgcaccg tcctcacgaa
     2101 aaaaggcgag cggagcatcc gccaggagag cctcgacctg atcttcggcc agctccgcaa
     2161 gggagccggc ggcgagcatc gcaccgccca ctccggcgaa gggggcgaga acctgccgga
     2221 aacgcgtcct tacgtgttcg gcgactcgat cagcgacctg gaccagatgc tgacgatccg
     2281 ctcggcgctc cgccacgggc tcgacgagat cgccatcacc gaggacgacc tccatgtcca
     2341 cgaaaaggaa catcgcacca gcgtggccac ggtgctctgc ctcgacatca gccacagcat
     2401 ggtgctgtac ggcgaggacc ggatcacgcc ggccaagcgg gtggctctgg cgctctccga
     2461 gctgatcctc acccgctatc ccaaggacag cctcgacatc ctcctcttcg gcgacgacgc
     2521 ccgcgtggtg ccactcgaag aactcctctc gagcggcgtg gggccgttcc acaccaacac
     2581 gaaggcggcg ctccggctgg cgcagcagat cctccgaacg aagaagcatg tccagaaaca
     2641 ggttttcatg atcaccgacg gcaagccgtc ggccatcctg gaagaaggtc ggatctacaa
     2701 gaattccttc ggcctcgacc cgaagatcgt gaaccgcacg ctggatgagg ccgtggcctg
     2761 ccgccggaag aacatcgcca tcaccacgtt catggtggcc aaggatcatg ccctggtgga
     2821 attcgtggag gagatgaccc gcctcaacaa gggtcgcgcc tactacacgg gcctcgacaa
     2881 cctcggcggc tacgtcttcg cggacttcgt caagaatcgg cggcgcgtgg tccgctgacg
     2941 gtagtttcgt cacgccgtcc acgcacaaac ttcacgcccg gcgtccctgc ctcactccct
     3001 ccgcgtcgtc tatccggcgc gcccccagta acttgccatc ctcccgccgg ggccggacgg
     3061 atgcgcatgg ttcttgcagc cctcgccaac ggaacggggc cctcccggcc tcgcgaggag
     3121 catcgccatg aacacctgcg aagtgtgcgg caacacctac gatcggacgt ttgaagtgat
     3181 ccaggaaggg aagagccacg tcttcgacag cttcgaatgt gccattcacg ccctggcccc
     3241 cacctgtgag cactgcggtt gccgcgtcgt cggccacggc gtgcaggacg agggtgccat
     3301 cttctgctgc gtccactgcg ccgaagaaag cggcgttacc ggactgcgtg atcgccaatg
     3361 atggaggacc gaacgatgac ggtacgagaa gccatgacca gccggacgga actggccacc
     3421 ccggacatga ccctgcgaga tgccgcccga ctcatgcgcg attgcgacat cggatcgctg
     3481 ccggtcggcg agaacgaccg gctgatcggc atgatcaccg atcgcgacat cacgatccgc
     3541 gcagtggccg aaggacggga tgtccacacc accccggtgc gcgacgccat gacgaccaag
     3601 gtgctctacg tgtacgacga tgagccgatc gaagtcgcgg cccagacgat ggccgacctt
     3661 caggtgaggc gcctccccgt gctcgaccgc gagcggcacc tggtcggcat cctggcattg
     3721 agcgacgtct cgatcgaagg ccctcccacc tccgccgccg aggccctgcg cggcgtgtcg
     3781 gagccgagcg catgatccgc ttccggcccc gcctccagta tggggcgggg ccggaaaaac
     3841 aatgtaacaa ccaccacgaa ggtggtaccc agcgcgcaag tcattgaaaa taaat
//
LOCUS       DASVOD010000685         5114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_985814, whole genome shotgun sequence.
ACCESSION   DASVOD010000685 DASVOD010000000
VERSION     DASVOD010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985814"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF720_14700"
     CDS             complement(<1..424)
                     /locus_tag="VF720_14700"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880662.1"
                     /translation="MTGSPFRGTRRWLRYAAGRSAVGLLSGLPRPAGWALGRFLGRVG
                     HAVVRRDRDILRHNLEFVHPEMSDAERDAFSRQVYERLGENIFDVASLPRWTPDERRS
                     RFDVVGFEHLENAIAARRGVLLVGGHQGAWELVAPALAD"
     gene            complement(543..1529)
                     /locus_tag="VF720_14705"
     CDS             complement(543..1529)
                     /locus_tag="VF720_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEX7880663.1"
                     /translation="MTPDTDRVLAAARNSLAAARDAISGLDASLGAPFLDAIERLGAI
                     RGRIVVTGLGKSGLVAAKIAATLSSTGSPAVFLHTSDALHGDLGVVTPDDAVLALSKS
                     GRSAELVQLLPLFKRIGVPIVAMVSDTASPMAQGADIVLPLGKVRDAGPEDLVPTAST
                     TAMMALGDALAVALMHRRGFDASALAFVHAGGVVGRQAGTTVEQLMHAGEALPMVSDT
                     ATLREALVEILKKKLGVTTIAAADGRLAGILTDGDLKRIFLSEKGATALESPVADMMV
                     RSPRAIAPAAPIAEAVRVMEDPARGFVTSLVVVDGASKAIGIIHLHDCLKPA"
     gene            complement(1544..2065)
                     /locus_tag="VF720_14710"
     CDS             complement(1544..2065)
                     /locus_tag="VF720_14710"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX7880664.1"
                     /translation="MTKVLFLDVDGTLTDGGIILGDTDDSRRFHAHDGVGIKLAGLAG
                     ITVIFLSARENRAAARRARELGVRSVLGAARKHEHVATLCAELGTTPAQAAFVGDDLQ
                     DISGMKVCGTAIAVGDAVAEVKAIANHVTTRNGGYGAVREAVEWLLKRDGRWEATLAQ
                     FLASSDFVVRPDR"
     gene            complement(2062..2904)
                     /gene="kdsA"
                     /locus_tag="VF720_14715"
     CDS             complement(2062..2904)
                     /gene="kdsA"
                     /locus_tag="VF720_14715"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005883054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEX7880665.1"
                     /translation="MAERLVRVGGDERPVLFGGKRLAVIAGPCVLESAEITRRVAGEM
                     KEACARLGLPYVFKASYAKANRSSVESFRGPGISTGLDQLAAIRSEFDVPVLTDVHEA
                     GEAGTAGSVVDILQIPAFLSRQTALIEAAAKTGKVVNIKKGQFLAPGDMERAAEKAVS
                     AGAAGVLLTERGTTFGYGDLVVDMRGFAIMAKSGWPTIFDITHSLQQPGGRTTGGKRE
                     FAMPLARAAVAAGCDAVFLETHPDPANALSDSATMLAIPEAIKVLESLSKIRDALDAG
                     EPWL"
     gene            complement(2916..4556)
                     /locus_tag="VF720_14720"
     CDS             complement(2916..4556)
                     /locus_tag="VF720_14720"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX7880666.1"
                     /translation="MSKYVFVTGGVVSSLGKGILSASLGHLLRARGLKVTLQKCDPYL
                     NVDPGTMSPFQHGEVFVLDDGAETDLDLGHYERFVDLNLTRSNNITTGQIYDTIIRKE
                     RQGDFLGRTVQVIPHVTDEIKRRIREIGDAEGVDVAIVEIGGTVGDIEGLPFLEAIRQ
                     MRLEERRDTLFIHLTYVPFLKAAREIKTKPTQHSVRELREIGIQPDILVCRSEASLSA
                     ALREKIALFCNVSKDAVIEAIDVPSIYRVPLMLHEAGLDRQVVELLDVKVGEPTLDFW
                     RGFVDTVENAPTETRIAVVGKYTHLRDAYKSIIEALAHGGVANNTRVDIEWVDSEEVE
                     THGASRLLGDVDGVLIPGGFGERGIEGKVTAVRWARETGTPFFGICLGMQCAAIEFAR
                     HVAGLKDANSSEFDRGTPHPVIDLMADQQTKTQKGGTMRLGSFECFVPTDTRTHAAYN
                     SELVHERHRHRYEFNNAYREMFERAGFRVAGVHRGLNLVEILELDDHPWFIGVQFHPE
                     LKSRPNRPHPLFAGFIKAAMAHAALSGGAIERPARHPA"
     gene            complement(4569..>5114)
                     /locus_tag="VF720_14725"
     CDS             complement(4569..>5114)
                     /locus_tag="VF720_14725"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEX7880667.1"
                     /translation="PECRTGSDRIAAALRALPHPDDRDDDIVINVQGDEPFIEPELIR
                     DLATVLENEPDTLMATVATKARPGDREDPATVTVALAHDDTALYFSRSALPGVGPGAT
                     EDAALAVSLRHLGLYAYRRAFLHEFVAWPPGKLEVVESLEQLRAVERGVRIRVIVRPT
                     ESIGIDTPADLERARRRLAGA"
BASE COUNT          802 a         1710 c         1770 g          832 t
ORIGIN      
        1 gatccgccag cgcgggggcc accagttccc aggctccctg gtgtccgccc acgagaagga
       61 cgccgcgtct tgcggcgatc gcattctcca gatgctcgaa cccgaccacg tcgaaccgcg
      121 agcgccgctc gtcgggcgtc cagcgcggca gcgaggccac gtcgaagatg ttctccccca
      181 gtcgctcgta gacctggcgg ctgaatgcgt cgcgctcggc gtcgctcatt tccggatgaa
      241 cgaactcgag attgtggcgg aggatgtcgc gatcccgccg gaccacggca tggccgacac
      301 ggccgaggaa tcgcccgagt gcccaccccg ccgggcgcgg caggccggac aacaggccga
      361 cggccgaccg accggcggcg taccgcaacc agcggcgtgt cccgcggaaa ggggagccgg
      421 tcatcgtccg gggtccccag tcatcgaagt tggcttgatc atctaataat aggtatcgga
      481 caggcggggt ccggtcaagc aactaatatg ccagtatttg caaattagtc ctgtggaacg
      541 aatcaggcgg gcttcagaca gtcgtgcaag tggatgatgc caatggcttt agaggcaccg
      601 tccaccacca ccagggaggt gacgaacccc cgggccgggt cttccattac ccgaaccgcc
      661 tccgcgatcg gcgccgccgg cgcgatggcg cgcggcgagc ggaccatcat gtcggccacc
      721 ggtgattcca gcgcggtggc ccccttctcc gacaggaaga ttcgcttcag gtcgccgtcg
      781 gtgaggatgc cggccagacg tccgtcggcg gccgcgatcg tcgtgacgcc gagcttcttc
      841 ttcaggatct cgacgagcgc ttcgcgcaac gtggcggtgt cggacaccat ggggagcgcc
      901 tctcccgcgt gcatcagttg ctccacggtc gttcccgcct gccgcccgac gacgccgccg
      961 gcatgcacga acgccagcgc gctcgcgtcg aaaccgcggc ggtgcatgag cgccacggcc
     1021 agcgcatcac ccagcgccat catcgccgtg gtcgaagccg tcggtacgag gtcttccggc
     1081 ccggcgtcgc gcacctttcc cagcggaagg acgatgtcgg cgccctgcgc catcggcgac
     1141 gccgtgtcgg acaccatcgc cacgatcggc acgccgattc gcttgaacag cggaaggagc
     1201 tgcacgagct cggcggaccg gccactcttg gagagcgcca gcaccgcgtc gtcgggcgtc
     1261 accacgccga gatcgccgtg cagcgcatcc gacgtatgca ggaaaaccgc cggcgaaccg
     1321 gtcgagctga gcgtggcggc gatcttggcg gccaccagcc ccgacttgcc gagcccggtg
     1381 acgacaatgc ggccgcggat ggcgccgagg cgttcgatgg catcgaggaa cggggcgccc
     1441 agcgaagcgt cgagtccgct gatggcatcg cgcgcggcgg ccagcgaatt gcgggcggcg
     1501 gcgaggactc ggtcggtgtc gggagtcatc gatccggtcc ggctcagcga tccggccgca
     1561 cgacaaaatc gctcgaagcc agaaactgcg cgagggtggc ctcccagcgg ccatcgcgct
     1621 tcaggagcca ttcgacggct tcccggacgg cgccgtaacc gccgttcctc gtcgtcacat
     1681 ggttggcgat ggccttcact tcggccacgg catcccccac cgcaatggcg gtgccgcaca
     1741 ccttcatgcc ggagatgtcc tggaggtcgt cgccgacgaa ggcggcctgg gcgggcgtcg
     1801 tgccgagttc ggcgcagagc gtggcaacgt gttcgtgctt gcgggcggcg cccagcaccg
     1861 aacgcacgcc gagctccctg gcccgtcgcg ccgcggcgcg attctcgcgg gcggacagga
     1921 agatgacggt gatgccggcc aggccggcca gcttgatgcc gacgccgtcg tgggcgtgga
     1981 agcggcggga gtcgtcggtg tcgccgagga tgatgcctcc gtcggtgagc gtgccgtcga
     2041 cgtcgaggaa caggaccttc gtcacagcca gggctccccg gcgtcgagcg catcgcggat
     2101 cttggacagc gattcgagca ccttgatcgc ctccggaatc gccagcatcg tggcgctgtc
     2161 cgacagggcg ttggccgggt cggggtgcgt ttcgaggaag acggcgtcgc atccggcggc
     2221 cacggcggcg cgggccagcg gcatggcgaa ctcgcgcttg ccgccggtcg tgcggccacc
     2281 cggctgctgg aggctgtgcg tgatgtcgaa gatcgtgggc cagccgctct tcgccatgat
     2341 ggcgaagccg cgcatatcta cgaccaggtc gccataaccg aaagtggtgc cgcgttcggt
     2401 caagaggacg ccggctgcgc cggcggacac tgccttttcg gcggctcgtt ccatgtcgcc
     2461 gggtgcgagg aattgaccct tcttgatgtt gaccaccttg ccggtcttcg ccgccgcttc
     2521 gatgagcgcc gtctgtcgcg aaaggaacgc ggggatctgc aggatgtcga cgacgctgcc
     2581 ggcggtaccg gcctcgccgg cttcgtgcac gtcggtgagg accggcacgt caaactcgct
     2641 ccggatggcc gccagttggt cgaggccggt cgaaatgccg ggcccgcgga acgactcaac
     2701 cgacgatcga ttggccttgg cgtagctggc cttgaagacg tacggcaggc ccaggcgggc
     2761 gcacgcttcc ttcatctcgc cggccacgcg ccgggtgatt tccgccgact cgagcacgca
     2821 cgggccggcg atgaccgcca ggcgtttgcc gccgaagaga accgggcgtt cgtcgccgcc
     2881 gacgcgaacc agtctttccg ccatgtggct cgggatcagg ccgggtgacg ggccggccgt
     2941 tcgatggcgc cgccggacag ggcggcgtgg gccatggccg ccttgatgaa gccggcaaag
     3001 agcggatgcg ggcggttggg gcgcgacttg agctcgggat gaaactgcac gccgatgaac
     3061 cagggatgat cgtcgagctc gaggatttca acgaggttca ggccgcgatg cacgcccgcc
     3121 acccggaagc cggcgcgttc gaacatctcg cggtaggcgt tgttgaattc atagcggtgg
     3181 cgatggcgct cgtgcacgag ctccgagttg tacgccgcgt gggtgcgcgt gtcggtgggg
     3241 acgaagcact cgaacgagcc gagccgcatc gtgcccccct tctgcgtctt ggtctgctgg
     3301 tcggccatga ggtcgatgac aggatgcggc gtgccgcggt cgaactcgct ggagttggcg
     3361 tccttgaggc cggccacgtg gcgggcgaac tcgatggctg cacactgcat gccgaggcag
     3421 atgccgaaga agggtgtgcc ggtctcacgc gcccagcgca ccgccgtcac ctttccctcg
     3481 atgccacgct cgccgaagcc gcccgggatg agcacgccgt ccacgtcgcc caggaggcgc
     3541 gaggcgccgt gcgtttccac ttcctcggaa tcgacccatt cgatgtcgac gcgggtgttg
     3601 ttggccacgc cgccgtgtgc cagcgcctcg atgatgctct tgtaggcgtc gcgcaggtgc
     3661 gtgtacttgc cgaccaccgc gatgcgcgtc tcggtgggcg cgttctccac cgtgtcgacg
     3721 aatccacgcc agaaatcgag cgtcggctcg cccaccttca cgtcgagcag ctccaccacc
     3781 tggcggtcga ggccggcctc gtggagcatg agcggcacgc ggtagatcga cggcacgtcg
     3841 atcgcctcga tgaccgcgtc cttcgacacg ttgcagaaga gcgcgatctt ctcgcggagc
     3901 gcggccgaca ggctggcctc cgaacggcac accaggatgt cgggctggat gccgatctcg
     3961 cggagctcgc gcaccgagtg ctgggtgggc ttggtcttga tctcgcgggc cgccttgagg
     4021 aacggcacgt aggtcaggtg gatgaagagg gtgtcgcgtc gctcctcgag ccgcatctgg
     4081 cggatggcct cgaggaacgg cagcccctcg atgtcgccga ccgtgccgcc gatctccacg
     4141 atcgccacgt ccacgccctc cgcgtcgccg atctcgcgga tgcgccgctt gatctcgtcg
     4201 gtgacatgcg ggatgacctg cacggtgcgt ccgaggaaat ccccctggcg ttccttgcgg
     4261 atgatcgtgt cgtagatctg gccggtcgtg atgttgttcg agcgggtcag gttcaggtcg
     4321 acgaagcgct cgtagtggcc gaggtcgagg tcggtctcgg cgccatcgtc cagcacgaac
     4381 acctcgccgt gctggaaggg actcatcgtg ccgggatcga cattgagata gggatcgcac
     4441 ttctggaggg tgaccttgag gccgcgggcg cggagcaggt gccccagcga cgcggagaga
     4501 attcccttgc cgagcgagga gacgacgccg ccggtcacga atacgtactt ggacatctca
     4561 tctccttctc aggcgccggc cagccgccgg cgggcccgct cgagatcggc cggcgtgtcg
     4621 atgccgatcg attcggtcgg tcgaacgatc acccggatcc tcacgccgcg ttccacggcg
     4681 cgcagctgct ccaggctttc caccacttcc agtttccccg gcggccacgc cacgaactcg
     4741 tggaggaaag ctcgccggta agcatacaac cccaggtgcc ggagcgagac ggccagggcc
     4801 gcatcctccg tggcccccgg ccccacgcct ggcagcgccg agcgcgagaa atagagggcc
     4861 gtatcgtcgt gcgccagcgc cacggtaaca gtggccgggt cttcccgatc gccgggacgg
     4921 gccttcgtgg ccaccgtggc catcagcgtg tccggttcat tctcgaggac ggtcgccagg
     4981 tcccggatca gctccggttc gatgaacggt tcgtccccct ggacgttgat cacgatgtcg
     5041 tcgtcccggt cgtccgggtg gggcaacgcg cgcagcgccg cggcgatccg gtcgctcccc
     5101 gtccggcact cggg
//
LOCUS       DASVOD010000686         7502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_463414, whole genome shotgun sequence.
ACCESSION   DASVOD010000686 DASVOD010000000
VERSION     DASVOD010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7502
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463414"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VF720_14730"
     CDS             complement(<1..273)
                     /locus_tag="VF720_14730"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880668.1"
                     /translation="MDLGLTGRVALVTGGSSGLGLAAATALASEGARVAIASRSRENL
                     EKAATAIHAATRRDVEGFEADVSELDQIPALTAAVRAKLGPVEILVA"
     gene            complement(281..1804)
                     /locus_tag="VF720_14735"
     CDS             complement(281..1804)
                     /locus_tag="VF720_14735"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEX7880669.1"
                     /translation="MNDLSLVIRELAGFVPHLMAAVSILILGWLGAWIVRALVERGLN
                     ALKTDERLASWGVGRLVGDDDARRGMSLSHAVATLLYFLLMLFVLMAFFDALQLTDFT
                     QPINRLLEEVMGYVPRFLGAVAVLILAWIVASLLRLIVLRGLQALGLDPRLEAATHTH
                     PEHHTSDTIATVIFWTVILFFLPAVLRVLDLEAAVIPLTSMFARALAAVPDIFAAGSI
                     LLIGWIVARVVRAVVVGFTEAMGLQGVAVRLGLTRLLGGQRITSILGTLAYVVTLIPV
                     LVASIDALHLDALSRPAVGALASLFNALPYLFGSVLILAMAFLFGRVLRDFVAGILRG
                     FGFDGILVSLGMSSSRAALAGRSPSQIGGTVVMAFVMVFAAMEAAEVMNLRNLADLIS
                     RFIEFFSQVVLSVVIVGIGFWLANFTRRIILAGSTGEGPDNANLLLGNLARYAIIVFA
                     FALGFQQMGIGKEIVLTAFALVLGAICLALALSFGLGSRELAQRTVENWARKLKSDR"
     gene            1867..3108
                     /gene="lysA"
                     /locus_tag="VF720_14740"
     CDS             1867..3108
                     /gene="lysA"
                     /locus_tag="VF720_14740"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006746491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX7880670.1"
                     /translation="MIDSAPGPAGLSVSGVPLADLASRFGTPLYVYSADTLLERYREL
                     DRAFAAIPHRLHYSVKANASRALLSLLAREGAGADVVSEGEMERALRAGFAPGHIVFA
                     GAGKTDAELARALGAKIGLINIESAGEMARLTRLAGDRRVSVAFRVTPDIVGGTHHYT
                     ETGTRATKFGIPEETALELAGQLAARRGAGPTLVGLHVHFGSQITRLEPFVAAAEKLA
                     ALVRAFRAKGADIEHVNMGGGLGIRYKDEEPPTPAALAGAVVPVYAGLEIELLLEPGR
                     WIVGPSGLLLATVIDVKTTGTDTFVVVDAGMNDLIRPSLYGAWHDIAPVVPRPGTARV
                     VDVVGPICETGDFLARGRELPPLEPGDVVAVRDAGAYGFSMASTYNSRPRPAEVLVDS
                     GRTRIIRRRETIDDLLVAESD"
     gene            complement(3132..5627)
                     /locus_tag="VF720_14745"
     CDS             complement(3132..5627)
                     /locus_tag="VF720_14745"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HEX7880671.1"
                     /translation="MAADRPPRRLVVHYHRPEGDEAGWNLWVWNDRTHATGRELAPAG
                     RDDFGLVFVVDLAPWARSGETIDPVGFLPRRNHWEERDGDDRVWRPGDPDAIWVMARR
                     PGWSGIRPDTSPFVIGGFLDQPDELLLEVYPALEPSALADTSFRVESTVMGVLDVASA
                     VPAGGDSWLGHRCHRVRVTLSAPIDCVGAPLAPLSVWADGWRPGVVWPRHILDRPEFQ
                     STAPLGAIVAGDATTWRVFAPTAAKVELRLYREATGGAPETVPMAREAGGTWCCRLPG
                     DRTGEWYMFRADGPDPRFRPDREFLDPYARAVSAYDGRARVVGPTAPPGAGPSFGVDE
                     AIVYEVHVRDLSASADSGITARGRYAGLAESGTHLPGDPGTTTGLDHMLELGINTVQV
                     LPVPAFRHAPGAYEWGYMPVLFQAPDGAYASGEDGANAVAEWKEAVDALHRRGLKVVM
                     DVVFNHTAELLPDRLYAYEGLAPGYYHRRHADGSFWNGSGTGNEFRSEAPMARRLILD
                     SLRTWIVDYGVDGFRFDLMGLIDLDTMKAIVRELRALKPDILLWGEPWTAAPSPIAPT
                     VAGMQRGQGFAVFNDHFRNAIRGDHAGAVKSFLAGGGRAGAFRRGLEGSVHDFADTPV
                     ESVNYVECHDNQTLWDRLASLPSTREMDEATRTRLHRLAGAAVLLARGIPFLHAGQEF
                     LRTKQGIDNSWDKGDEINRIEWTRKRRHADTVGWHRDLIRLRRAHPLLRRSPGPGEPR
                     DLLWLDDDLKLAVPPPVAALLLGRGPVADFWSEAVLILNPAADTHDVPLPPGAWRVVI
                     DGDHVVDAPLSDLPAANERIPVPPRSLILLARE"
     gene            5647..6513
                     /locus_tag="VF720_14750"
     CDS             5647..6513
                     /locus_tag="VF720_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880672.1"
                     /translation="MSNRPSSGRRATPSRITSDRRATSPRLGRLEVREDHEGIKLMAH
                     GTLYAVLPPTPRQTPPPWLALAASPWMHDAGAPEAPIGLLGYGGGTVARLLRAADRRA
                     VILGVDLDPDVLRLGRRHLRSAVSGVRVDQADAREWLRRPGPEFRALLDDLYAPAGGF
                     LARPAELESIPALARKRLAPGGIYAVNLRCPPGREEKQIVLRIRRLFREVRVIHTLEY
                     AHKVVVATDRPIRPGALGRALARLLEPANRNGSLPADAGSCNPRTLGFRTVIRLPHGS
                     DRHATSKSRSNE"
     gene            6585..>7502
                     /locus_tag="VF720_14755"
     CDS             6585..>7502
                     /locus_tag="VF720_14755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7880673.1"
                     /translation="MRKRWIFGTLVVALLAAGGWYATRPRTKAPEYRTAVVDRGTIAA
                     QITATGTVKPVTMVQVGSQVSGTIQSLHADFNSTVRKGQVVAQLDPSLFRTQLAQNEA
                     NLDRARVNVKDAERTFARSKELLARNLIPQAEVDAAEAAVDRFKAEVSQARATVEQSR
                     VNLEHTTIQSPIDGVVVSRSVDVGQTVAASLSAPVLFEIANDLSAMQVEASIDEADIG
                     QIKAGQPATFTVDAFPDEKFTGVVSQTRLQPITVQNVVTYTTVIAVGNPDLKLRPGMT
                     ANVSVETDRREDVLRVPAGALTFKPPDARG"
BASE COUNT         1253 a         2605 c         2532 g         1112 t
ORIGIN      
        1 cgccacgagg atctcgacgg ggccgagttt cgcgcggacg gcggccgtga gcgcggggat
       61 ctgatccagt tcggagacgt cggcttcgaa tccctcgaca tcccgccggg tcgccgcgtg
      121 gatggccgtg gcggccttct cgaggttttc cctcgaccgg gaagcgatgg ccacccgggc
      181 gccttcgctg gccagtgcgg tggcggcggc gagccccagc ccgctcgatc cccccgtgac
      241 cagcgccacg cgtccggtca ggccgaggtc catcgatttc tcagcgatcc gacttgagct
      301 tgcgggccca gttctcgaca gtccgctgag ccagctcgcg gctcccgagg ccgaacgaca
      361 gggccagcgc caggcagatc gcacccagca ccagggcgaa cgccgtcagg acgatctcct
      421 tgccgattcc catctgctgg aagccgaggg caaaggcaaa gacgatgatg gcgtagcggg
      481 ccaggtttcc gaggaggagg ttggcgttgt cgggtccttc gccggtcgat ccggccagga
      541 tgatgcgccg ggtgaaattg gccagccaga acccgatgcc gacgatgacc accgacagca
      601 ccacctgcga gaagaactcg ataaagcggc tgatcaggtc ggccaggttc cggaggttca
      661 tcacctcggc ggcttccatc gcggcgaaaa ccatgacgaa ggccatgacc accgtgccgc
      721 cgatctgcga ggggctgcgt cccgccaggg cggcccgcga actcgacatc cccagtgaaa
      781 cgaggatgcc atcgaagccg aagccgcgca ggatgccggc cacgaagtcg cgcaggacgc
      841 ggccgaacag gaacgccatc gccaggatca gtaccgagcc gaacaggtag gggagtgcgt
      901 tgaagaggga cgccagcgcg ccgacggccg gccgcgagag agcatcgagg tggagcgcgt
      961 cgatcgaggc caccaggacc ggtatcagcg tgaccacgta ggccagggtc ccgaggatgc
     1021 tggtgatgcg ctggccgccg agcagccggg tgagcccgag ccgcaccgcc accccctgca
     1081 ggcccatcgc ctcggtgaag ccgacgacca cggcgcgcac gacacgggcc acgatccagc
     1141 cgatcaggag aatcgacccg gcggcgaaga tgtccggcac cgcggccagc gcacgggcga
     1201 acatcgaggt gagcgggatc accgcggcct cgaggtcgag caccctgagc acggccggca
     1261 ggaagaagag gatcaccgtc cagaagatga cggtggcgat cgtgtccgac gtgtggtgct
     1321 cgggatgcgt gtgcgtggcc gcctcgagcc tcgggtcgag cccgagggct tgaagcccgc
     1381 gcagcacgat cagccggagg agggaggcca cgatccaggc caggatgagg accgccaccg
     1441 ccccgagaaa gcgcggcacg taccccatca cttcttccag caggcgattg atgggctggg
     1501 tgaagtcggt gagctgaagc gcgtcgaaga acgccatcag cacgaagagc atgaggagga
     1561 agtagagcag cgtggccact gcgtgggaga gcgacatgcc gcggcgcgcg tcgtcgtcgc
     1621 ccaccagccg accgacgccc catgatgcga gccgttcgtc ggtcttgagc gcgttcagtc
     1681 cccgctcgac aagcgcccgg acgatccagg cgccgagcca tcccaggatg aggatcgaga
     1741 cggcggccat gaggtgcggg acgaagcccg cgagttcgcg gatgacgagc gaaaggtcgt
     1801 tcatctccat gatccgtgta gtgaacgggt taacgcgccg ccagtcaagc ggctatgatg
     1861 gccgtcatga tcgattccgc gcccggtccg gccgggcttt ccgtgagcgg cgtgccgctc
     1921 gccgacctgg cttcccgatt cgggacgccg ctttacgtct attccgccga cacgctgctc
     1981 gagcgttacc gcgaactcga ccgggccttt gccgccatcc cgcaccggct ccactactcg
     2041 gtgaaggcca acgcctcccg cgccctcctc tcgctcctgg ccagggaggg ggcgggcgcc
     2101 gacgtggtgt ccgaaggaga gatggaacgg gcactccggg ccggctttgc gcccgggcat
     2161 atcgtcttcg ccggcgcggg aaagaccgac gccgagctgg cccgtgccct gggggcgaag
     2221 atcggcctca tcaacatcga atcggccggc gaaatggcgc ggctcacgcg gctggctggt
     2281 gaccgtcggg tgtcggtggc cttccgggtc acacccgaca tcgtcggcgg gacgcaccac
     2341 tacaccgaga cgggcacccg ggccaccaag ttcggaatcc ctgaagagac ggcactcgaa
     2401 ctggccggcc agttggcggc ccgtcggggc gccggtccca cgctcgtggg gctgcacgtg
     2461 cacttcggtt cgcaaatcac ccggctcgag ccgttcgtgg ccgcggcgga aaaactggcg
     2521 gccctggtcc gcgccttccg cgccaaaggc gccgacatcg agcatgtcaa catgggcggg
     2581 ggcctcggca tacgctacaa ggacgaggag ccacccacgc ccgccgccct ggcgggagcg
     2641 gtcgtcccgg tctacgcggg gctcgagatc gaattgctcc tcgagcccgg gcgctggatc
     2701 gtcggccctt cgggcctcct cctcgccacg gtcatcgacg tcaagaccac cggaaccgac
     2761 acgttcgtcg tggtggacgc cggaatgaat gacctgatcc gcccctcgct ctacggcgcc
     2821 tggcacgaca tcgcgccggt cgtgccgcgc ccgggtaccg ccagggtggt cgatgtcgtc
     2881 ggaccgatct gcgaaacggg ggatttcctg gcccgcgggc gcgagttgcc accgcttgaa
     2941 ccgggagatg tcgtggccgt ccgcgacgcc ggtgcgtacg ggttcagcat ggcatccacc
     3001 tacaacagcc ggccgcgtcc ggccgaggtg ctggttgatt cgggccggac ccggatcatc
     3061 cgccgccgcg aaacgatcga cgacctgttg gtggccgaga gcgactgaac cgggcgggcg
     3121 atgcttcccc gttactccct ggccaggagg atcaggctcc gcggcgggac gggaatccgt
     3181 tcgttcgcgg ccggcaggtc cgacagcggt gcatcgacga cgtgatcgcc atcgatcacg
     3241 acccgccacg cgccgggcgg caggggaacg tcgtgtgtgt ccgccgccgg gttcagtatc
     3301 agcacggcct cgctccagaa gtccgccacc ggaccccgcc cgaggagaag tgccgccacg
     3361 ggaggcggca cggcaagctt gagatcgtcg tcgagccaga ggaggtcgcg cggttcacca
     3421 ggtcccgggg aacgtcgaag cagggggtgc gcccgtcgga gccggatcag gtcgcggtgc
     3481 caccccacgg tgtcggcatg tcgccgcttc cgagtccatt cgatccggtt gatctcatca
     3541 cccttgtccc acgagttgtc gatcccctgt ttcgtccgga ggaattcctg gccggcatgc
     3601 aggaagggga tgccgcgggc caggagcacg gcggcccctg ccagccgatg gagccgcgtg
     3661 cgcgtcgcct cgtccatctc acgagtggaa gggagcgagg ccagccgatc ccagagggtc
     3721 tgattgtcgt ggcattccac gtagttcacc gattcgacgg gcgtgtcggc gaagtcgtga
     3781 acgctccctt ccaggccgcg gcgaaaggca ccggcgcggc ctccgccagc caggaacgat
     3841 ttgacggcgc cggcatggtc tccccggatc gcattgcgga aatgatcgtt gaagaccgcg
     3901 aatccctggc cccgttgcat ccccgcaaca gtcggcgcga tcggcgacgg ggcggcggtc
     3961 cacggttcgc cccagagaag gatgtccggc ttcaacgcgc ggagttcgcg gacgatcgcc
     4021 ttcatcgtgt cgagatcgat caaccccatc aggtcgaagc ggaagccatc cacgccgtag
     4081 tcgacgatcc acgtccgaag cgaatcgagg atgagccggc gcgccatcgg tgcctcgctc
     4141 cggaattcgt tgccggtgcc cgagccgttc cagaacgatc cgtccgcgtg acgccggtgg
     4201 tagtagccgg gagccagccc ctcgtatgcg tagaggcggt cggggagaag ttctgccgtg
     4261 tggttgaaca ccacgtccat caccaccttg aggccgcggc gatgcagggc atcgacggct
     4321 tccttccatt cggcgacggc gttcgcaccg tcctcccccg aggcataggc gccgtcggga
     4381 gcctggaaca gcaccggcat gtaaccccac tcatacgcgc ccggcgcgtg gcggaatgcc
     4441 ggcacaggca atacctgcac cgtgttgatg ccgagctcga gcatgtggtc aagcccggtg
     4501 gtggtgcccg gatcgcccgg gagatgcgtg cccgattcgg ccagccccgc gtatcggcct
     4561 cgcgccgtga tgccggagtc ggcgctcgcg ctcaggtcgc ggacgtggac ctcgtaaacg
     4621 atggcttcgt ccacaccgaa cgatggtccg gcccctggag gcgcggtcgg cccgacgacg
     4681 cgtgcccggc catcgtatgc cgacacggcg cgggcatacg ggtcgaggaa ttcgcgatcg
     4741 ggccggaaac gggggtcggg cccatcggcc cggaacatgt accactcccc ggtccgatct
     4801 cccggtagcc ggcagcacca ggtgccgccg gcctcgcggg ccatcggcac ggtttcggga
     4861 gcaccacctg tcgcctcccg atagagtcgc aattccacct tcgccgccgt cggcgcaaag
     4921 acccgccagg tcgtggcgtc gcccgccacg atcgcgccga gcggggcggt gctctggaac
     4981 tcgggacgat cgaggatgtg ccgcggccac acgacgccgg gtcgccaccc gtcggcccac
     5041 acactcagcg gcgcgagtgg cgctccaacg caatcgatcg gtgccgacaa cgtcacgcgc
     5101 acgcggtgac aacggtgccc cagccacgag tcgccgccgg ccggaaccgc gctcgccacg
     5161 tcgagcaccc ccatcaccgt cgattccacg cggaagctcg tgtccgccag cgccgagggc
     5221 tcgagcgcgg ggtacacctc gagcagcagt tcgtccggct ggtcgaggaa gccgccgatc
     5281 acgaagggag atgtgtcggg ccggattccg ctccaccccg gccgcctggc catgacccag
     5341 atggcatcgg ggtcgcccgg tcgccagacg cgatcgtcgc cgtcgcgctc ctcccagtga
     5401 ttgcggcgcg gcaggaaacc gaccgggtcg atcgtctcgc cgctccgggc ccagggagcg
     5461 aggtcgacta cgaagacgag tccgaagtcg tcgcgcccgg ccggcgccag ctcgcgaccg
     5521 gtcgcgtgcg tgcgatcgtt ccatacccag aggttccacc ccgcttcgtc accctcgggg
     5581 cggtggtagt ggacgacgag tcgccggggc ggtcgatcag cggccatctg tcgtattctc
     5641 ggcggaatgt ccaatcgacc atcgtccggg cgtcgcgcca cgccgtcccg catcacgagc
     5701 gaccgccggg ccaccagtcc ccggcttggt cggctcgagg tccgcgagga ccacgaggga
     5761 atcaagttga tggcgcacgg aaccctctac gccgtgctgc cacccacccc ccgccagacg
     5821 ccccctccct ggctggccct ggctgcctct ccctggatgc acgacgccgg cgcgcccgag
     5881 gcgccgatcg gcttgctcgg ctacggtggc ggaacggtgg cgcggctcct gcgggccgcc
     5941 gatcgacggg cggtgatcct gggcgtcgac ctcgatcccg acgtgctccg gctcggccga
     6001 cgccatctcc gttcggcggt atccggcgtc cgggtcgatc aggccgatgc ccgggagtgg
     6061 ctcaggcgcc cgggtcctga attccgggcc ctcctcgacg acctctacgc cccggctgga
     6121 ggattcctgg cccgtcctgc cgaactcgaa tcgatcccgg ctctggccag gaagcgcctt
     6181 gccccgggag gaatctacgc ggtcaatctt cgttgtcccc ccggccgcga ggaaaaacag
     6241 atcgtcctcc ggatccgtcg actctttcgc gaggtccgcg tcatccacac attggagtac
     6301 gctcacaagg tggtggtagc cacggatcgg cccatcaggc ccggggcact cgggagggcc
     6361 ctggcccggt tgctggaacc ggccaatcga aatggtagtc ttccggccga cgcgggatcc
     6421 tgcaacccgc gaaccctggg ctttcgaacc gtgatccgcc tgcctcatgg ttcggaccga
     6481 cacgctacct ccaaatcgcg atcgaacgaa taacgacccc tgtcccgtcc gggccggtgc
     6541 gattcctgtc cgggcgtgat gcggcatcaa cctgggagac cctcgtgcgc aagcgctgga
     6601 ttttcggaac tctcgtcgtg gccctgctcg cggcgggtgg ctggtatgcc acccgtcccc
     6661 ggacaaaggc gccggagtat cgcaccgccg tggtcgatcg cggaacgatc gcggcgcaga
     6721 tcaccgccac cggcaccgtg aagccggtca cgatggtcca ggtcgggagc caggtgtcgg
     6781 gcaccatcca gtcgctccat gccgacttca attccacggt gaggaagggg caggtggtgg
     6841 cccagctcga tccttctctc ttccgcacgc aattggccca gaacgaggcc aacctggacc
     6901 gcgcgcgggt caacgtgaag gacgccgagc ggaccttcgc gcggagcaag gagctcctgg
     6961 cccgcaacct catcccgcag gccgaggtcg atgccgcgga agccgcggtc gatcgtttca
     7021 aggccgaagt cagccaggcc cgggccacgg tggagcagtc ccgggtcaac ctcgagcaca
     7081 ccaccattca gtcgcccatc gacggggtgg tggtctcgcg cagcgtcgat gtcggccaga
     7141 ccgtcgcggc cagcctctcg gcgccggtcc tcttcgagat cgccaacgac ctctccgcca
     7201 tgcaggtgga agcgtcgatc gacgaggccg atatcggcca gatcaaggcc ggccagccgg
     7261 ccaccttcac cgtggatgcc tttcccgacg agaagttcac gggcgtcgtt tcgcagacgc
     7321 ggctgcagcc gatcaccgtc cagaacgtcg tcacctatac cacggtcatc gcggtcggca
     7381 atccagacct gaagctgcgc cccggaatga ccgccaacgt atcggtcgaa accgaccgcc
     7441 gcgaagatgt cctccgcgtg ccggccggcg ccctcacgtt caagccgccc gacgcgcgcg
     7501 gc
//
LOCUS       DASVOD010000687         7704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_669829, whole genome shotgun sequence.
ACCESSION   DASVOD010000687 DASVOD010000000
VERSION     DASVOD010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669829"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF720_14760"
     CDS             <1..460
                     /locus_tag="VF720_14760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7880674.1"
                     /translation="SAGKGESNRKAFKKRVADGPPPGLIAYAGRTPIGWIAIGPRASL
                     PRLNNSRILAPVDEQPVWSVSCFFVTKEWRKRGVSVFLIEAAARFAAKAGAKLVEGYP
                     VVARGTAPAPFVWTGLPGTFLEAGFEEVARRSTTRPIMRRAVTKAMSKGK"
     gene            741..3563
                     /locus_tag="VF720_14765"
     CDS             741..3563
                     /locus_tag="VF720_14765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HEX7880675.1"
                     /translation="MTRRLPLLRHLVPFLVLALGIAAAEGVLAAPGDIDPTFGTGGTA
                     RIHTGFGDDFGNAMLPLAGDGCLVAGSTQEGGTGQIFVAQYLSNGALNSSFGTGGVSR
                     LNPPPGQSGVGASCLTLQADGKIVVGGSVSSTPVVARFLSNGTPDSTFGTNGLVTLPG
                     VYAAAIAGVAMQGSKIVIGGGFRAGTKYHFGLIRLNSNGSLDTTFDGDGIVSTPMLVE
                     SFARALLLQSDGKIVLAGNAANTSLKNEVAVVRYNADGTLDTGFDGDGKTTIAVGPGG
                     SYVYALANQDTRLLVAGMTQSTSSGPFNALLIRYNANGTLDPTFGPASGTGILIQPVG
                     AYDDAFRGVAVTYNSRGSVNGIIAAGSTAATSGNTSDVLVCRYLVTGALDTSFDGDGI
                     VVTSTSSRQDQAYGMALLGSKILLVGYSGFGATDADVVLLRYVATGGALDTGLDGDGI
                     RIHDIGDEYAGAASIGVAPDGKVVLTGTISTNPSRGVAARLTPGGAIDTSFGVNGIAL
                     FSLPGPGVSVRDHVMQSDGRLLLVGSARGSASSGMLMLRLLPDGALDPSFAAVGHREW
                     SPGAGFCEVTGVALRPDGRSILVGYTGTSGGQAYVGRFDDAGAPDLAFGSNGQVLLQP
                     GGYPASAEGVAIQPDGKLVIVGTTAPTGSSLRILVLRLTANGALDPTFGGSGFVTTDV
                     SPQSDYGTSVEILGDGKIVVSGNQGVTSSASGTCILRFDSHGVPDPTLDGDGIRTQSS
                     GTEKTSVYAVRSMPDGHFLMAGSVTSPGGSSYDQRVLNVRYDGAADPGWSGDGSVTLD
                     FASPSDAASDLALVADGSVLVAGTGGGIVAVTRLAGAFSATGVGDLEPPQLANVGPAW
                     PNPTPLGATLAYTLPAPSTTSAAVFDVAGRRVRLLRSEAVEPEGNRSIHWDGHDENGE
                     EVGRGVYFIRLRAGDQVVARRVVVAR"
     gene            complement(3527..4420)
                     /locus_tag="VF720_14770"
     CDS             complement(3527..4420)
                     /locus_tag="VF720_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880676.1"
                     /translation="MRFSPLDRALAARILQRWQVGDLPESPSRRLDLLHAACLRRVPF
                     ENLTKLIHRATAPTIEAARRPAPVFWREHLEMGAGGTCFSTTEAFGQLLAAVDLEARP
                     IFCHLRAERRHAHVALIVETEHGPRLCDTGYALAAPMPLPGRLAVRRKTRHYEMELRP
                     GVDGEHLLFLEDWKGRRFRYQFRDVEITPEQWFGAWDDSLAPGAVYMNRLALGRFGVA
                     VRWILHPDNRVMVLARRGERAIVLPGDERAADRRLARLFQVPEMMLAGARKVLRDGLS
                     STAAELPATPYLATTTRRATT"
     gene            4513..5184
                     /locus_tag="VF720_14775"
     CDS             4513..5184
                     /locus_tag="VF720_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880677.1"
                     /translation="MSNDRVVLVGGSMGALGSAMTRVFLEDGATVGALYMGTPPPAHE
                     RVIPLEADLTDTAAVARAVTSLLAEAGRIDILVNAAGGFAAGKVIDTTDEAWDRQLAL
                     NLKIPIILSRAVLPHMTARGEGRIWHVAARAARHPFPGAAAYVVSKTALLGLIRVLAL
                     ELAGTGVTANAILPGTLDTPANREGMPNADTSRWVPTEELARILLGLCSPESRHLNGE
                     TIVIG"
     gene            5280..6410
                     /locus_tag="VF720_14780"
     CDS             5280..6410
                     /locus_tag="VF720_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880678.1"
                     /translation="MKKRQRPLSSRLVLAAISVLGVSALYWTGCGTEDQAPVSAPNRT
                     QFTEELNYANPRVQATMAVQDRHTADIMQDNNVVGTATGVADDGTMVIKVYLREAAGT
                     RDYIAELEGVPVEYEVTGQFTAFKGGGGGGGSDPKVKQTPPIKLGTSGGWRYDLANGY
                     CCGGTLGALVLKGGNQYVLSNYHVLEADIVNGGNSRVAQAGDPVIQPGLIDVNCTAAN
                     AQNVATLSGIKSLPNSNTDCAIAQVTPGMVSTTGEILNIGTLSATTQAASLGQAVKKM
                     GRTTGLTRSSVSGLNASVSVAYENECAGGSAFTKTYSGQIVIANSGSRFLAGGDSGSL
                     LVQDVTTNPRAVGLLFAGSSSAAIANPIGQVLSFLGASMVGN"
     gene            6427..6849
                     /locus_tag="VF720_14785"
     CDS             6427..6849
                     /locus_tag="VF720_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880679.1"
                     /translation="MSIETPAPGNGGGRSLWLALAVMALGGLAALVAIVVLARRDAGR
                     ARPSGDDIVATALSAESSREEGSLSIEDATLVLERHKDQLMQLPGVIGIYVGADHQSK
                     LVLRVLLLHGHESTRAQIPAVLDGVPVEVEASDPIKPL"
     gene            complement(6853..>7704)
                     /locus_tag="VF720_14790"
     CDS             complement(6853..>7704)
                     /locus_tag="VF720_14790"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7880680.1"
                     /translation="YAQLRVIDKDELRHFIIDGGVHTMVYPGDWQTMHRYAIVTTSLT
                     KLFPGHGRVLVVGLGGGSVVKAYRSAGWDVSAVEIDPMVTRFAHDYFGLDPSEAEVTH
                     MDARRFLRKKNGTWDLIVGDAYGSSSVPWQLLTREYFEQMKERLNEGGVVALNLECVG
                     WDDILIRSAAATLKTSFRYVRALPTSEPPDALGNIMLLASDRPIDIGDTIAAFGHPKD
                     FVPDTYRHFAVLQELHAWDNAYEPKTEGAPIMTDDRTPVDLWSERINWVARKDLHEYF
                     AKDGTSW"
BASE COUNT         1234 a         2632 c         2590 g         1248 t
ORIGIN      
        1 ctcggccggc aagggggagt cgaaccgcaa ggcgttcaag aagagagtgg ccgatggccc
       61 accgcccgga ctcatcgctt acgctgggag gacgcccatc ggctggatcg ccatcgggcc
      121 acgcgcgtcg ctgccgcggc tcaacaactc gcgcatcctg gctccggtgg atgaacagcc
      181 ggtctggtcg gtgtcctgtt tctttgtcac gaaggagtgg cggaagcggg gcgtgagcgt
      241 cttcctgatc gaggccgcgg cgcgtttcgc ggccaaggct ggcgcgaagc tggtggaggg
      301 ttatcccgtc gttgcgcgcg gcaccgcgcc cgcgccgttc gtctggacgg ggttgccggg
      361 aacgttcctc gaggcgggtt tcgaggaggt ggcgcggcga tcgacgacgc ggcccatcat
      421 gcggcgggcc gtgacgaaag cgatgtcgaa gggcaaataa tgtgttgccg cgacgcggcg
      481 acatcaatcc gcaaccgaca tgtcctcctg gtgttctttc cgcctgcccg caatttcccc
      541 aaatcacccc ccgcaatccc tctcccgccc gtggcacccg tcccgccgat tcggcgcatg
      601 gagagtgacc tcatccggca accggacgcc cccgagaggc ccctgatgcc ttaactgttt
      661 gaggtgaaac acattgcgtc gcaggccgat tcggctttcc tgccgtggcg acgcgaactg
      721 ccgaaagtga agggacgaca atgactcgac gcctcccgtt actgcgccac ctggtccctt
      781 tcctcgtcct ggccctcggc atcgccgcag ccgagggagt cctcgccgcc cccggcgaca
      841 tcgaccccac gttcggaacc gggggcaccg cccggattca caccggcttc ggggatgact
      901 tcggcaacgc catgctgccg ctcgcgggcg acggctgcct ggtggccggc tccacccagg
      961 aggggggcac ggggcagatc ttcgtggccc agtacctgag caacggcgcg ctgaacagct
     1021 cgttcgggac ggggggcgtg agccggctca atccaccgcc gggacagtcg ggcgtgggtg
     1081 cctcgtgcct gacgctgcag gccgacggga agatcgtcgt cggggggagc gtctcgagca
     1141 cgccggtcgt ggcgcggttc ctttcgaatg gaacaccgga ctcgaccttc gggaccaacg
     1201 ggttggtgac acttccgggc gtctatgccg ccgccatcgc gggtgtcgcc atgcaggggt
     1261 caaagatcgt gatcggcggc gggttccgtg cgggcacgaa gtaccacttc gggctgatcc
     1321 gcctgaattc gaatgggtcg ctcgatacga ccttcgacgg cgatggaatc gtcagcacgc
     1381 cgatgctcgt ggagagcttc gcccgggcgc tgctgctcca atcggacggc aagatcgtcc
     1441 tggccggaaa cgccgcgaac acgagcttga aaaacgaagt tgccgtggtt cgctacaacg
     1501 ccgacgggac gctcgacact ggcttcgacg gggatggaaa gacgacgatc gcggtggggc
     1561 ccggaggctc gtacgtctac gccctcgcca accaggacac gaggctcctc gtggccggga
     1621 tgacgcagtc cacctcgtcc ggacccttca acgcacttct catccggtat aacgccaacg
     1681 gcaccctcga ccctacgttc ggcccggcgt cgggaacggg catcctgatc cagccggtcg
     1741 gggcctacga cgacgccttc cggggcgtgg cggtcacgta caacagtcgc ggcagcgtca
     1801 acggcatcat cgccgccggc tcgacggccg ccaccagcgg caacacctcc gacgtgctcg
     1861 tctgccgata cctggtgacg ggtgcgctgg acacctcctt cgatggcgac gggatcgtcg
     1921 tgacctccac gagcagccgc caggaccagg cgtacggcat ggcgctcctc ggctcgaaaa
     1981 ttctcctggt cggctacagc ggcttcggag cgacggacgc ggacgtcgtc ctgctccgct
     2041 atgtcgccac gggcggcgcc ctcgacacgg gcctggacgg tgatggcatc cgtatccacg
     2101 atatcggcga tgagtacgcc ggggccgcgt cgatcggcgt ggctcccgac ggcaaggtgg
     2161 tcctgacggg aacaatctcc accaacccgt ccaggggcgt tgcggcccga ctcaccccgg
     2221 gcggggccat cgatacgagc ttcggcgtga atggcatcgc cctcttctcg ctccccgggc
     2281 cgggtgtttc ggtcagggac cacgtgatgc agtcggatgg tcgccttctc ctggtcggat
     2341 cggcccgggg gtccgccagc tcgggaatgc tcatgctgcg cctcctgccc gacggggcgc
     2401 tcgacccctc cttcgcagca gtcggtcatc gggagtggtc gccgggcgcg ggcttctgcg
     2461 aggtcaccgg cgtcgcgctc cgccccgatg gacggagcat tctcgtcggt tacacgggca
     2521 cgtcgggtgg gcaggcttac gtcgggaggt tcgatgacgc cggtgctccc gacctcgcct
     2581 tcggaagcaa cggtcaggtg ttgctccagc cgggcgggta tccggcctcc gccgaaggcg
     2641 tggccattca gccggacggc aagctcgtca tcgtcggcac caccgcgccc accggtagct
     2701 cgcttcgtat cctggtcctc cgactgacgg ccaacggcgc actggatccc acgttcggtg
     2761 gctccggttt cgtcacgacg gacgtgagcc cgcaatcgga ctacggcacc tccgtcgaga
     2821 tccttggcga cggaaagatc gtggtgagcg gtaaccaggg ggtcacgtct tcggcgtccg
     2881 gcacgtgcat cctccgcttc gacagccacg gggtgccgga ccccacgctc gatggcgacg
     2941 gcatccgcac gcaatcttcg ggaaccgaga agacgtccgt ctacgcggtt cggtcgatgc
     3001 ccgatgggca cttcctgatg gccggaagcg tcacctcgcc gggcggatcc agctacgacc
     3061 agcgcgtgct gaacgttcgc tacgatggcg ccgccgaccc ggggtggagc ggcgacggga
     3121 gcgtcacgct cgacttcgcc tccccttcgg acgcggccag cgacctggcg ttggttgccg
     3181 acggcagcgt tctcgttgcg ggaacgggcg gcggtatcgt tgccgtgacg aggctggccg
     3241 gcgcgttttc tgccaccggt gtcggtgacc tcgaaccccc gcaactcgcc aacgtcgggc
     3301 cggcctggcc caaccccacg ccgctcggcg ccacgctggc atacacgctg ccggccccgt
     3361 caacgacttc ggctgcagtc ttcgacgtgg ccggacgacg cgtgcgcctc cttcgatcgg
     3421 aagcggtgga gccggagggg aaccggtcca tccactggga tggacacgat gaaaatggag
     3481 aagaggtcgg gcggggtgtc tacttcatcc gactgcgcgc cggcgatcaa gtcgtcgcgc
     3541 ggcgcgtcgt cgtggcgaga tagggagtcg cgggcaactc ggccgcggtt gaagacaaac
     3601 catcgcgcaa caccttccgc gcccccgcca gcatcatctc cggcacctgg aagaggcgcg
     3661 ccagccggcg gtctgcggcg cgctcatcgc cggggagcac gatcgcgcgt tcgccgcggc
     3721 gcgcgagcac catcacgcga ttgtccggat gaaggatcca gcgtaccgcc acgccgaagc
     3781 ggccgagcgc gagccggttc atgtacaccg caccgggggc cagtgaatcg tcccacgcgc
     3841 cgaaccactg ctcgggcgtg atctcgacgt cgcggaactg gtagcggaag cggcgcccct
     3901 tccagtcttc caggaagaga aggtgctcgc cgtccacgcc ggggcgcagt tccatctcgt
     3961 aatggcgcgt cttgcggcgc acggccagtc gccccggcag cggcatcggc gcggcgagcg
     4021 cgtaaccggt gtcgcagagg cgggggccgt gttcggtctc cacgatgagg gccacgtggg
     4081 catggcgtcg ctcggcgcgg aggtggcaga agatcggcct ggcctcgagg tccactgcgg
     4141 ccaggagctg cccgaatgcc tccgtcgtgc tgaagcaggt gccgcccgct cccatttcga
     4201 gatgctcccg ccagaagacc ggcgcggggc gacgagccgc ctcgatggtc ggggccgtgg
     4261 cgcgatgaat cagcttggtg aggttctcga acggcacgcg gcgcaggcag gcagcgtgca
     4321 ggaggtcgag gcgccgggaa ggggattcgg ggaggtcgcc gacctgccag cgttggagga
     4381 tacgggcggc cagcgccctg tcgagtggag aaaatcgcat aggttccgga ccgaccgggc
     4441 gggaagttca ccacgccggg ggctaccgag gcaagcggaa aggggaaggg ccggtgctag
     4501 actgccggcc tcatgtccaa cgatcgggtg gttctcgtgg ggggctcgat gggcgccctc
     4561 ggctcggcaa tgacccgcgt cttccttgag gatggggcca cggtcggcgc cctgtacatg
     4621 ggcaccccgc cgccggccca cgagcgggtc atccccctcg aggcggatct cacggatacc
     4681 gcggcggttg cgcgggccgt cacctcgctc ctcgcggagg ccggccgcat cgacatcctg
     4741 gtcaacgccg ccggcgggtt cgcggccggg aaggtgatcg atacgaccga tgaggcctgg
     4801 gaccggcagc tcgcgctcaa cctgaagatt cccatcatcc tgtcgcgggc ggtcctcccg
     4861 cacatgaccg cgcgcggaga agggcgtatc tggcacgtgg ccgcccgggc ggcccggcac
     4921 cccttcccgg gggccgcggc ctacgtcgtc tccaagacgg ccctcctcgg cctcatccgc
     4981 gtgctggccc tcgagctggc cggcaccggc gtgacggcca acgccatcct gccggggacg
     5041 ctcgatacgc cggccaaccg ggaagggatg cccaacgccg ataccagccg ctgggtgccc
     5101 accgaggagc tggcccgcat cctgctcggg ctctgctcgc cggaatcccg ccatcttaat
     5161 ggcgaaacaa ttgtgatcgg atagcacgaa atagattgcc tcggacccca ccccgggagt
     5221 taactcgaaa ggttcgttct cttcccttct cccccgcagt gcaatttttg gaggcaggta
     5281 tgaagaaacg ccaacgtccc ctctcctccc gcctggttct cgccgccatc tccgtcctcg
     5341 gggtatccgc cctgtactgg acgggttgcg gtacggaaga ccaggcaccg gtctcggctc
     5401 cgaaccggac gcagttcacc gaggaactca actacgcgaa cccgcgggtg caggccacga
     5461 tggcggtcca ggaccgtcac acggccgaca tcatgcagga caacaacgtg gttggaaccg
     5521 caaccggcgt cgccgacgac gggacgatgg tgatcaaggt ctatctccgc gaagccgcgg
     5581 gcacgcgcga ttacatcgcc gagctcgagg gcgtcccggt cgaatacgaa gtcaccggcc
     5641 agttcaccgc cttcaagggc ggcggtggcg gcggcggctc cgatcccaag gtcaagcaga
     5701 cgccgccgat caagctcggc acctccggcg gctggcgtta cgacctggcc aacggctact
     5761 gctgcggcgg cacgctcggc gcgctcgtcc tgaagggcgg caaccagtac gtgctgtcga
     5821 actaccacgt cctcgaggcc gacatcgtca acggcggcaa cagccgcgtg gcgcaggccg
     5881 gcgacccggt catccagccg ggcctcatcg acgtgaactg cacggcggcc aacgcccaga
     5941 acgtggccac gctgtcgggc atcaagtccc tcccgaactc gaacaccgac tgtgccatcg
     6001 cccaggtcac acccggcatg gtgtcgacca ccggtgaaat cctgaacatc ggcaccctct
     6061 ccgccacgac ccaggccgct tcgctgggcc aggccgtgaa gaagatggga cgcaccaccg
     6121 gcctcacccg cagctcggtg tccggcctca atgcctcggt cagcgtggcg tacgagaacg
     6181 agtgcgccgg cggttcggcc ttcacgaaga cctattcggg ccagatcgtc atcgccaaca
     6241 gcggcagccg gttcctcgcc ggcggcgact cgggttcgct cctggtccag gatgtcacca
     6301 cgaatccccg ggccgtcggc ctgctcttcg cgggcagctc gtcggccgcc atcgccaacc
     6361 cgatcggcca ggtgctctcg ttcctgggcg cctcgatggt cggcaactga cgaacggcat
     6421 ttttcgatga gtatcgagac gcccgctccc ggaaacgggg gcgggcgttc tctttggctg
     6481 gcgctggccg tcatggccct gggaggcctg gccgcgctcg tggccatcgt cgttctggcc
     6541 cggcgcgacg ccggccgggc acgcccgtcg ggtgacgaca tcgtggccac cgcgctttcg
     6601 gccgagtcgt cgcgcgagga ggggagcttg tcgatcgaag acgccacgct cgtgctggag
     6661 cgtcacaagg atcaactgat gcagcttccc ggggtcatcg gcatctacgt cggcgccgac
     6721 caccagtcga agctggtgct gcgcgttctc ctgctccacg gacatgagtc cacccgggcg
     6781 cagatccccg cggtgctgga tggcgtgccg gtcgaggtcg aggcttcgga cccgatcaag
     6841 ccgctgtagc agctaccagc tcgtcccatc cttcgcaaag tactcgtgca ggtccttccg
     6901 cgccacccag ttgatccgct ccgaccagag gtccaccggc gtccggtcgt cggtcatgat
     6961 cggcgccccc tccgtcttcg gctcgtaggc attgtcccag gcgtgcaact cctgcagcac
     7021 ggcaaagtgg cgataggtgt cgggcacgaa atccttggga tgcccgaacg cggcgatcgt
     7081 gtcgccgatg tcgatcgggc gatcgctggc caggagcatg atgtttccca gcgcatccgg
     7141 cggctccgag gtcggaaggg cgcgtacgta gcggaagctg gtcttcagcg tggcggccgc
     7201 ggagcggatc aggatgtcgt cccagccgac gcattccagg ttcagcgcca cgacgccccc
     7261 ctcgttgagc cgttccttca tctgctcgaa gtactcacgc gtcaggagct gccagggaac
     7321 cgagctggag ccgtaggcat ctcccacgat caggtcccag gtcccgttct tcttgcgaag
     7381 gaagcggcgg gcgtccatgt gggtgacttc cgcttccgaa gggtcgagcc cgaagtaatc
     7441 gtgggcgaag cgggtgacca tcggatcgat ttcgacggcg ctcacgtccc agccggccga
     7501 ccggtacgcc ttcaccaccg aaccgccccc cagccccacc accagcacgc ggccgtggcc
     7561 cggaaagagc ttcgtgagcg acgtggtgac gatggcgtag cggtgcatcg tctgccagtc
     7621 gcccggatag accatcgtgt ggacgccgcc gtcgatgatg aagtggcgca gctcgtcctt
     7681 gtcgatgacg cgcaactggg cata
//
LOCUS       DASVOD010000688         3714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_1041263, whole genome shotgun sequence.
ACCESSION   DASVOD010000688 DASVOD010000000
VERSION     DASVOD010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041263"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1573)
                     /locus_tag="VF720_14795"
     CDS             complement(2..1573)
                     /locus_tag="VF720_14795"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX7880681.1"
                     /translation="MTTELKRYRGAYEADFRGAWRACGVSELEEALAGADMVLSGDYH
                     TLPASQRLLIRLLRRLLPRDDRPATLALEMLHSEDQPVVDRYLAGRMGLEALREAISF
                     DHRWGFDWRHYGALLRFARERKLMVLGINVEPGVARGRLLKRDEHAARILVDHWQAAP
                     ERRIHLLAGDWHVARAHLPRLIRAEAKARGLRPSLLVIHQNHERLHARLDRPAGAPAV
                     ARRGRDLFCVLNTTPLVKADSNRLWASRQESSDDARARFESGEWPLFDPTLAFREVDE
                     VVARHLKLGPAPPFELVGPDDVPGLERTARRCDAPAGRATEWRKRLADGKVVWVPKAR
                     TLLLGHAPIHRLAEEAMRRRLEAPTSPWQLRLVRLDRDGTGASSSGVEARFHRRLRIE
                     GAAFLASKLVNPDRKCRLFADWNRTEGGLAGAWVRAWIRAARRQPGRLPFTAPDHPFW
                     TIRAATRLNVARAVGKAWGWHFFQAWTAGSLPWESVTALVDPIVDEGHDRRVISAGVS
                     AFPDDVDRHSKETLL"
     gene            1688..1957
                     /locus_tag="VF720_14800"
     CDS             1688..1957
                     /locus_tag="VF720_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880682.1"
                     /translation="MARHDYHFVRNREFWTLYLTMIVAALAWGAFWAVTATYVFRLNR
                     QALTFLVCICMASALSLGVTIYIRLRFLVAEASRRVFERELDLDL"
     gene            2087..2368
                     /locus_tag="VF720_14805"
     CDS             2087..2368
                     /locus_tag="VF720_14805"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX7880683.1"
                     /translation="MRLSERKIRYLSDRIVSWMEKRGDVEFIDRPEVVALAVARAVTQ
                     ELKQEDDLDDEVEKVLKDYQNQIRGQNMDLMLLRQKIKMQLARERGIVL"
     gene            2365..2646
                     /locus_tag="VF720_14810"
     CDS             2365..2646
                     /locus_tag="VF720_14810"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HEX7880684.1"
                     /translation="MRLNEKRIDRMAAAILDHLLEEDLVDFKPTTRQMRSELSRFITQ
                     DLAIEDEITQEAMEKLNTYSRKIPEGTTEWMLLLAKHKDEIAAKRGYTL"
     gene            2778..>3714
                     /locus_tag="VF720_14815"
     CDS             2778..>3714
                     /locus_tag="VF720_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880685.1"
                     /translation="MDDLKRWREERAARRSDRRMRHASLRETPTADSPVEGHSQLSLF
                     GATVPVTIANTAPVGRTESITSTAQRILRTRRTVSSAPPPMVSTNPPHVGVLRRRELL
                     ARASHERRGASNVPINWMPEPERISPVHPVWDIPRNKRLRAGLIASLVFHAVAIPLVL
                     LGTKIDFGIFNRKEPTERTIASVTLRNVIPMTKGQGGQGLRKIENKPPPKPPETPPQN
                     KKHSSPPPPKPKPTKQTNSKPAESVDLTKSRVKPVEDKSNKATRPSPETTKPVPTPAI
                     SNDVVAGPLLAAKGPVPGSELAIGGANGVDFPYNYY"
BASE COUNT          681 a         1269 c         1197 g          567 t
ORIGIN      
        1 gttacaacaa tgtctccttg ctatgccgat ccacgtcgtc cgggaaagcc gacacgcccg
       61 cggagatcac gcgccgatcg tgtccttcgt cgacaatcgg atcgaccagg gcggtaaccg
      121 attcccacgg gaggctcccg gccgtccacg cctggaaaaa gtgccagccc caggccttgc
      181 caaccgcgcg ggcaacattg agtcgggtcg ctgcgcgaat cgtccagaac ggatgatccg
      241 gggccgtgaa gggaagcctc cccggctggc ggcgcgcggc gcgaatccag gcacggaccc
      301 atgcgccggc cagcccccct tcggtgcggt tccagtcggc gaagagtcgg cacttgcggt
      361 ccgggttgac cagcttcgaa gccaggaacg ccgcgccttc aatgcgaagg cggcggtgga
      421 accgcgcctc cacgccggat gatgacgccc ccgtcccgtc gcgatcgagg cgcacgagcc
      481 tgagttgcca tggcgaggtg ggggcctcga gccggcgtcg catggcctcc tcggccagcc
      541 ggtgaatcgg cgcgtggcca agcagcagcg tgcgcgcttt cggtacccac accaccttcc
      601 cgtcggccag gcgcttgcgc cattcggtcg cacgcccagc cggggcatcg caccggcgcg
      661 cggttcgctc gaggcccggc acgtcatcgg gcccgaccag ctcgaacggg ggagcgggtc
      721 cgagcttcag gtggcgcgcc accacctcat ccacttcgcg gaaggccagc gtcgggtcga
      781 acaacggcca ctcgcccgat tcgaagcgcg cccgcgcgtc atcggacgat tcctgccgtg
      841 atgcccacaa ccggttcgaa tcggccttca cgagcggcgt ggtgttgagc acgcagaaga
      901 gatcacgtcc ccggcgggcc accgcggggg cgcccgccgg cctgtcgagt cgggcgtgga
      961 gtcgttcgtg gttctggtgg atgacgagaa ggctgggcct caagccgcgc gccttcgcct
     1021 cggcgcggat caatcgcggc aggtgggccc gcgccacgtg ccagtcaccg gccaggagat
     1081 ggatgcgccg ttcgggcgcg gcctgccagt gatcgaccag gatccgcgcc gcgtgttcgt
     1141 cgcgcttgag gaggcgcccg cgggccacgc ccggctcgac gttgatcccg aggaccatga
     1201 gcttcctctc gcgcgcaaac cggagcaggg cgccgtagtg cctccagtcg aacccccacc
     1261 ggtgatcgaa cgagatcgcc tcgcgaagcg cctccaggcc catgcggccg gccaggtagc
     1321 ggtcgaccac cggctggtcc tcggagtgca gcatctcgag tgccagcgtg gccggccggt
     1381 cgtcgcgcgg caggagtcgg cgcaacagac ggatcaggag tcgctgggag gcggggagcg
     1441 tgtggtaatc acccgagagc accatgtcgg cgcccgcgag agcctcctcc agttccgaca
     1501 ccccgcaggc ccgccaggcg ccccggaaat cggcctcgta ggctccccgg taccgtttca
     1561 gttcggtggt catcaggccg ataacgtctc gaaggtcgtc ctgccacctg gatatcagac
     1621 tgccgtggag ccgggcgatt cgttcccggg cccggcgtgc gcggcgtgtc cgcagagagg
     1681 agtcagggtg gcgcgtcatg attatcattt cgtcaggaac cgggaattct ggacccttta
     1741 cttgaccatg atcgtggccg ccctcgcctg gggtgctttc tgggccgtca cggccacgta
     1801 cgtcttccga ctgaaccgcc aggccctcac ctttctcgtc tgcatctgca tggcctccgc
     1861 cctcagcctc ggggtcacca tctatattcg gctacgtttc ctggttgcgg aagcctcccg
     1921 gcgcgttttc gaaagggaac tcgatctgga tctttgagcc actcctcgta agcttctgaa
     1981 aatcaatgag ttctccgggt ccattcaagt gacttcgact tgacagctgc ccccgccgcg
     2041 gctaattttg aggcagaatc gattctcggc ccgcggggag cccgacgtgc gactgtccga
     2101 acgaaaaatc cgatacctgt ccgaccgtat cgtctcctgg atggagaagc gtggggatgt
     2161 cgagttcatc gaccgccccg aggttgtggc gcttgccgtc gcccgcgccg tcacgcagga
     2221 actgaagcag gaagacgacc tcgatgacga agtcgagaaa gtgctcaagg actaccagaa
     2281 ccagatccgc ggccagaaca tggacctgat gctcctgcgc cagaagatca agatgcagct
     2341 ggcccgcgaa cggggcatcg tcctgtgagg ctcaacgaaa agcggatcga ccgcatggcc
     2401 gcggccatcc tcgatcacct gctcgaggaa gatctggtcg atttcaagcc gacgacgcgc
     2461 cagatgagga gcgagctttc gcgcttcatc acgcaggacc tggccatcga agacgagatc
     2521 acgcaggaag cgatggagaa gctcaacacc tactcgcgca agattcccga gggaaccacc
     2581 gagtggatgc tcctcctggc caagcacaag gacgagatcg ccgcgaaacg cggctacacg
     2641 ctgtaaccgc ggccgcccga cggcggccag gcgcggacgg cccggggggt gccggtctgc
     2701 gtggcaaaac gcaacattca tcaatgtggt cccggacggg ggaaccgtaa ctcccggaac
     2761 ccggtacaag gggacccatg gacgatctga agcgctggcg cgaggaacgg gcggcccgtc
     2821 gaagcgatcg acggatgcgc catgcatccc tccgggaaac ccccacggcc gacagtcctg
     2881 tcgagggcca ttcgcagctc agcttgttcg gcgccacggt gcccgtcact atcgccaaca
     2941 ccgccccggt gggccgcacc gagtccatca cgagcacggc gcagcgcatc ctccgcacgc
     3001 gtcgcaccgt gtcgtccgcg ccgccgccga tggtgtccac gaatccgccg cacgtgggcg
     3061 tcctgcgccg gcgcgagctg ctcgcgcgcg catcgcacga acggcgcggc gcctcgaacg
     3121 tcccgatcaa ctggatgccc gagcccgagc gcatctcacc cgtgcatccc gtgtgggaca
     3181 tcccgcgcaa caagcgactg cgggcggggc tgatcgcgag cctcgtcttt cacgcggtgg
     3241 ccatcccgct ggtgctcctg gggacgaaga tcgacttcgg gattttcaac aggaaggagc
     3301 cgacggagcg gacgatcgcg tcagtaacac ttcggaacgt catccccatg accaagggcc
     3361 agggggggca ggggctccgc aagatcgaga acaagccgcc tcccaagccc ccggagactc
     3421 ctccccagaa caagaagcac tcgtcgccgc cgccgcccaa accaaagccg acgaaacaga
     3481 cgaactcgaa gccggccgaa tccgtcgatc tcacgaagag tcgcgtgaag ccggtggaag
     3541 acaagtcgaa caaggccacc aggccgtcac cggaaaccac gaaacccgta ccgacgcccg
     3601 ccatttcgaa cgatgtcgtg gcgggtccgc tcctggctgc caagggaccg gtgcccggaa
     3661 gcgagctggc gatcgggggg gccaacggcg tcgactttcc gtacaactac tacc
//
LOCUS       DASVOD010000689         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_827837, whole genome shotgun sequence.
ACCESSION   DASVOD010000689 DASVOD010000000
VERSION     DASVOD010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827837"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..724)
                     /locus_tag="VF720_14820"
     CDS             complement(344..724)
                     /locus_tag="VF720_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX7880686.1"
                     /translation="MLKKPRIPERNAFTPVWRLVKRIPAGCVMTYGQISELLGRRLSA
                     RAVGWAMAGCPKDVPWWRVVNARGMCSTETLVEPPGLQKRLLGKEGVRFNDRGRLDLE
                     ARRWHPRARGGISKPAAKSKGRKG"
     gene            complement(729..>2604)
                     /locus_tag="VF720_14825"
     CDS             complement(729..>2604)
                     /locus_tag="VF720_14825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX7880687.1"
                     /translation="DLWMADVAGGEPARFTSGGKDAHPRWSPDGSRIAFIASRDKGRP
                     QIWTIPAMGGEAVKLTEFPEGVLASFRWSPDGSQLAASFREADPEWTEAAGKNRAEKG
                     LSDPPRVLDDWWYRLDGDGYFNAQRFRLYVIDAATGKHRLLYDQDNMGGFSFDWSPDG
                     REIALTTNRSARAMVEPWKDEILRIDVKSRKLRAVPNLPEGSKTMVRWSPDGKTLAWA
                     GREGKMGSWDAENIELFVGSPTRGGARSLTRKTDYCLAAATLADTAEAAFDPQIVWTP
                     DSKRIVMRLGWHGEGHLASVARTGGPVTLHTSGAREHGMGNLDAKGSRIALLVGDSSS
                     IAEIHVADVTKAWGKLAPTALTAFNAPLLKELEIARPKSHWIRAADGHKVQVWSIRPV
                     RPRRKMPAILEVHGGPHAQYGVPFFHEFQLLAAQGYAVFYSNPRGSKGYGRYHCAVIR
                     GDWGNKDWVDIQAVIDFMKIQPDVDARRMGIMGGSYGGYMTNWAIGHTRAFKAAITDR
                     CVSNLVSMMGNTDFVEAPDFYWQGDFWNRSEKFRAMSPIVYFDKVKAPTLIIHSEGDL
                     RCNIEQAEQVFSVLKLRKVPTRFVRYPRSTSHGMSRGGPPDMRLHRLRQILAWWKKYL
                     "
BASE COUNT          414 a          920 c          823 g          447 t
ORIGIN      
        1 aacacccctt ccatgccgcg aatggtgcgg tgcaggcggg tgtcgtcact gacgcccgat
       61 tgcgaacggg tcttcggcgc gagggcgacg agcagcgccc ccgccaggct cacggtgaga
      121 agggtgcgcc cgatgcgggc agccaggggg gaacgcgcaa cgccaaacat gatgggaatc
      181 tcctgagtcg gtggtggtct ttacggcccc ggaaggctcc gggacccggc ccattcgtcg
      241 ttcaggagtc acagacgcac ggcatgcggc gtttgttgaa gaggggtcat ggtgttgacg
      301 tgccggccgg acaccgcacg gcctgaccgc aggggcgatc cggtcagcct ttgcgcccct
      361 tggacttggc ggccggtttc gaaatcccgc cacgtgcccg gggatgccac cgccgcgctt
      421 cgagatccag tcggccgcgg tcgttgaacc gcacgccttc ctttcccagc aaccgcttct
      481 gcaagcccgg cggctcgacc agtgtttccg tcgagcacat cccccgcgcg ttcaccacgc
      541 gccaccacgg cacatctttc ggacaaccgg ccatggccca cccgaccgcc cgcgccgaca
      601 gccgtcgtcc aagcagctcg ctgatctggc cgtaggtcat cacgcatccg gcggggatgc
      661 gcttgaccag ccgccagacc ggggtgaagg cgttccgctc gggtatcctt ggcttcttca
      721 gcaccctgct agaggtactt cttccaccac gccagaatct gccgcagccg gtgaagccgc
      781 atgtcgggcg gtccgccccg gctcatgccg tggctggtgg agcgcgggta gcgcacgaag
      841 cgcgtcggca ccttgcgcag cttcagcacg ctgaagacct gctcggcctg ctcgatgttg
      901 cagcggaggt cgccttccga gtgaatgatg agcgttggcg ccttcacctt gtcgaagtag
      961 acgatcggac tcatcgcccg gaacttctcg gagcgattcc agaaatcgcc ctgccagtaa
     1021 aagtcaggcg cctccacgaa gtcggtgttc cccatcatgc tcacgaggtt cgacacgcag
     1081 cgatcggtga tggccgcctt gaaggcgcgc gtatggccga tggcccagtt cgtcatgtag
     1141 ccgccgtagc tgccacccat gatccccatg cgcctggcat ccacgtcggg ctggatcttc
     1201 atgaaatcga tgaccgcctg gatgtcgacc cagtccttgt tcccccagtc gccccggatc
     1261 acggcgcagt gataccgccc gtaccccttg ctgccgcgcg gattggaata gaagacggcg
     1321 tacccctgcg cggccaggag ctggaactcg tggaagaacg gcacgccgta ctgcgcatgg
     1381 gggccgccgt gcacctcaag aatggcgggc atcttccgcc ggggacgaac cgggcggatc
     1441 gaccagacct gcaccttgtg gccgtcggcc gcgcgaatcc agtggctctt ggggcgcgcg
     1501 atctccaact ccttgaggag cggcgcgttg aaggcggtga gcgccgtcgg cgccagcttt
     1561 ccccaggcct tcgtcacgtc ggccacgtgg atctcggcaa tcgagctcga gtcgcccacg
     1621 aggagcgcga tccggctacc tttcgcgtcg agatttccca tgccgtgctc gcgcgcacca
     1681 ctcgtgtgca gggtaaccgg cccgcccgtg cgcgccaccg acgccaggtg cccctcgccg
     1741 tgccagccga ggcgcatcac gatccgcttg ctgtccggcg tccagacgat ctgcggatca
     1801 aaggccgcct cggccgtatc ggccagcgtg gcggccgcca ggcagtagtc ggtcttgcgg
     1861 gtgaggctcc gcgcgcctcc cctggtggga ctgccgacga agagttcgat gttctcggcg
     1921 tcccagctcc ccatcttccc ttcgcgcccc gcccaggcca gcgtcttccc gtcgggcgac
     1981 cagcggacca tcgtcttgga accttcgggg aggttgggca ccgcgcgcag cttccgagac
     2041 ttcacgtcga tccggaggat ctcgtccttc cacggctcca ccatggcccg cgccgagcgg
     2101 ttggtggtga gcgcaatctc cctgccgtcc ggcgaccagt caaaactgaa gccacccatg
     2161 ttgtcctggt cgtacagcag gcggtgcttg ccggtagccg cgtcgatcac gtaaagccgg
     2221 aagcgctggg cattgaagta gccgtcgcca tccaggcgat accaccagtc atccagcacg
     2281 cgcggcggat cactcagtcc cttctccgcg cgattcttcc cggccgcttc ggtccactcg
     2341 ggatccgctt cgcggaacga cgccgcaagc tgcgacccgt cgggcgacca gcggaacgac
     2401 gcgagcacac cttcggggaa ttccgtgagc ttgaccgcct cgccgcccat ggcgggaatt
     2461 gtccagatct gcggccgacc cttgtcacgc gaggcgatga acgcgatccg cgacccgtcg
     2521 ggggaccagc gcgggtgcgc atccttcccc cccgacgtga accgcgccgg ttcgcccccc
     2581 gcgacatccg ccatccagag gtcg
//
LOCUS       DASVOD010000690         6098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_827849, whole genome shotgun sequence.
ACCESSION   DASVOD010000690 DASVOD010000000
VERSION     DASVOD010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827849"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VF720_14830"
     CDS             complement(<1..1242)
                     /locus_tag="VF720_14830"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX7880688.1"
                     /translation="MNPTLTLQTQYGPDPAIQGAPAPGGRRKHERLVLLRTSMVLLDL
                     LAIAGSMAVAMTLGKAGIRIGPGLLPVALLSAAIWIGSVAWAGGYEVRLLGRRLELTT
                     RLLKASILAVILQILVDFSLRPAFPMPGRGALMIFWLSATALVLTLRWSVWRWVVVRA
                     LRGRLISSAVVVGADPESQRRSALILRWMLFPPVVRRVIDGEGVTEEIERLAASGSID
                     QVLVVREDIPRDTLIELVQRCVGWGLTVVVASPAFNVMIGRAPVFVLDGLPVIEIQPS
                     GLFTPARQIKRGLDLVGALIGGTLLLPLFALIALAIKLTSRGPVFYRQERVGRDGRVF
                     HFFKFRSMVVNNDEHVHRAYLENLVRNGAEATLDRAGQKVYKLVDDPRVTPIGAFLRR
                     TSLDELPQLWNIIKGEMSLVGP"
     gene            complement(1471..2550)
                     /locus_tag="VF720_14835"
     CDS             complement(1471..2550)
                     /locus_tag="VF720_14835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX7880689.1"
                     /translation="MHLDTAGLKFIPDDLLLVVSYRCNSRCVMCGIWEGDQSSKGEMT
                     PEEYRATIPDTLTHVNITGGETFLRRDLVDVVAAVKEAAPKAAMTISTNGLQPSLTRR
                     LLPDILRQKPDIGFAVSIDGDETMHDTVRGIPGNYNKCIETVRAIQEAGSTNVRIGFT
                     AMGKNDHQLTTIYRLAKELGVQFTCAVAHNSDHYFKTHANHGVHADVLAKELDTVARE
                     ELRSTNVKSWFRAYFQKGLGEFAATGQRPVECEAAHDSYMMDPKGFIFPCNILNMPIG
                     NVREQSFREIWTSQKMDEIRHVVRNCQEPCWMVCTARSAIKRHLPEVSTWVLVHKLKA
                     HFGMTVIPGPGGSPKDPSAAALSGN"
     gene            complement(2659..3837)
                     /locus_tag="VF720_14840"
     CDS             complement(2659..3837)
                     /locus_tag="VF720_14840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880690.1"
                     /translation="MSTPGRPLRVAMIGSKGIPATVGGVERIVEELSARLGERGHQVS
                     VYARRGYVGSAAPERHRGVRVRVTPSLPGKYSDTLSHAFASVLDGLPRGFDIWHVHSL
                     GNAPVIPLLRAAGARVLFHVHGQEWKGGKWGKKAQDYFRRCERLGLAAAQRTVVNTLA
                     SRDDYQARLGRESTFIPNAVEVPEPRPTGILERLGVPPDGFLLFVGRLVPEKGCHHLI
                     EAHRRLKLDLPIVVCGEGAHSDDYVARLKASAGPSVRFGGTILGAELTELYAACRLLV
                     NPTERDAVSLVLLEAMGQGAPVLASDIPEMLEGVGGAGFHFRSGDASDLARVLGDLLA
                     HPERLEGVRGLARERVLREYAWPSVVDRFEAVYREMMRARVEKMRRGPDHSGSPGPGR
                     "
     gene            complement(3834..4745)
                     /locus_tag="VF720_14845"
     CDS             complement(3834..4745)
                     /locus_tag="VF720_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880691.1"
                     /translation="MNILVTGGLGTVGSVLVAELRRRGNNVWIVDLPHHHDAQYLRAD
                     VGLYRQIERVFEGRQFDVVYHLAAEFGRWNGEDFYDTLWRSNVVGTKNLIRLQEAHRF
                     RMVFFSSSEVYGDYDGVMAEDVMDKVEIRQLNDYAMTKWVGEQQCLNSAAMHGTETVR
                     VRLFNTYGPGEHYSTYRSVCCLFIYRALTDQPYKVYLNHHRTSSYVDDTVRTLANIAE
                     RFKPGAVYNIGGEEYHDIRQLSDLILKACGKTDRLVEYVEAEPFTTKDKKVDLTLSRR
                     DLGHAPQVPLAEGIPLTVAWMKSVYGL"
     gene            complement(4742..6028)
                     /locus_tag="VF720_14850"
     CDS             complement(4742..6028)
                     /locus_tag="VF720_14850"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX7880692.1"
                     /translation="MSAPRVAMVAIVDFPNGVGGDTRRVHMLARSLVAAGMTTALVIP
                     CTRGLVPPPAKVQGPEIIDGIHVIRLARQGSYGHTPGIGTAGMARLFLLRWYSLFLSL
                     VALVRLRIAGYRNFHLYQPTFYDGAAYWLLARLTGARVSADYSDLSFVDHDRIETNPA
                     RRLWSLNFRLGMTWLPARLDRTFVISHYLFASMAQAVRPERLRRIPPVVDTAAFDVPD
                     PDAPWRRRFGMPEGRVVLYAGAFFDNEGVPSLLAAAPRFLDGRPDVTLVVVGGHPADA
                     WQRCRDSVAGLVAADRIRFTGVRDSLEMPSCFRAADVLVAPKADHLLNRAGVPTKLVE
                     YLASGRPVVITRVGDIPELVTDGVDAVLVDPGDVTALGDAIAGLLDDPARALRLGQAG
                     RERARRDYDVAVIGKRLRKELVPGPAGTNERTHHTT"
BASE COUNT         1048 a         1959 c         2147 g          944 t
ORIGIN      
        1 cgggccgacc aggctcatct ctcccttgat gatattccaa agctggggga gttcgtcgag
       61 agaagtgcgc cgaaggaagg ccccgatcgg ggtgacccgc gggtcgtcca ccagcttgta
      121 gaccttctgg cccgcgcggt cgagggtggc ttccgcccca ttccggacca ggttttcaag
      181 gtaggcccgg tggacgtgct catcattgtt gaccaccatc gaccggaact tgaagaagtg
      241 aaagacccgg ccgtcccggc cgacccgctc ctggcggtag aaaacggggc cgcgggaggt
      301 gagcttgatc gccagggcga tgagggcgaa gagcggcagg aggagcgtgc cgccgatgag
      361 ggcgccaacg aggtccaagc cgcgcttgat ctggcgggcc ggggtgaaaa gacccgacgg
      421 ctggatctcg atgaccggga ggccgtcgag cacgaagacc ggcgcacggc cgatcatgac
      481 gttgaaggcc ggcgaggcga ccaccaccgt gagtccccag ccgacgcagc gctgtaccag
      541 ctcgatgagg gtgtcgcggg ggatgtcttc ccggaccacc aggacctggt cgatcgagcc
      601 ggaggccgcc aggcgttcga tctcctcggt gaccccctcc ccgtcgatga cacggcggac
      661 caccggcgga aacagcatcc agcgaaggat aagggcgctc cggcgctggc tctccgggtc
      721 ggcgcccacc acgacggcgg agctgatcaa ccgaccacgg agggcccgga ccaccaccca
      781 ccgccagacg ctccagcgga gcgtgaggac cagggccgtg gccgagagcc agaagatcat
      841 gagggcgcca cgaccgggca tcgggaaggc ggggcggagc gagaagtcga cgaggatctg
      901 gaggatgacg gccaggatgg aggccttgag gagccgggtg gtgagctcga ggcgccggcc
      961 gaggagccgg acctcgtagc cgcccgccca ggccaccgag ccgatccaga tcgccgccga
     1021 gaggagggcc acggggagga gcccggggcc gatccggatg ccggccttcc ccagtgtcat
     1081 ggcaaccgcc atggagccgg cgatggcgag gaggtcgagg agcaccatgg aagtgcggag
     1141 gagaacgaga cgctcgtgct tccggcgccc gccgggagcg ggcgccccct ggatggccgg
     1201 atccggcccg tattgagttt gcagggtcag cgtagggttc acggactcac catcggataa
     1261 ctcggctcac cagggtctgt cagcacgcag tcgttggccg gtctcgagac gggatccact
     1321 accgaccatg aaacggtacc acagtgacgt tgcggccaac aacgagagtc catccggtga
     1381 ccatgcccga ccgcacggtc taagttcaac tatctcaatg tctaatgtgg tcggcgcccg
     1441 gaaatccgta caccccgcgg gggaaaacga tcagtttccg gacagggccg cggccgatgg
     1501 gtccttgggt gagccacccg ggccgggaat gacggtcatg ccgaagtgcg ccttgagctt
     1561 gtggacgagc acccaggtgg aaacctcggg gaggtgccgc ttgatggcgc tccgggcggt
     1621 gcagaccatc cagcagggct cctggcagtt ccgcacgacg tgccggattt cgtccatctt
     1681 ctggctggtc catatctcgc ggaacgactg ctcccggacg ttgccgatcg gcatgttgag
     1741 gatgttgcag gggaagatga atcccttcgg atccatcatg taggaatcgt gggccgcctc
     1801 gcactccacc gggcgctggc ccgtggccgc gaactcgccg agccccttct ggaagtaggc
     1861 ccggaaccac gacttgacgt tggtggagcg gagctcctcc cgggccaccg tatcaagttc
     1921 cttggccagg acgtcggcgt ggacaccgtg gttggcgtgc gtcttgaagt agtgatcgga
     1981 attatgggcc accgcgcagg tgaactggac gcccagctcc ttggccagcc ggtagatggt
     2041 ggtgagctgg tggtcgttct tccccatggc cgtgaagccg atgcggacat tggtggagcc
     2101 ggcttcctgg atggcccgca ccgtctcgat acacttgttg tagttgcccg ggatcccgcg
     2161 gaccgtgtcg tgcatcgttt cgtccccgtc gatcgagacg gcgaagccga tgtccggctt
     2221 ctggcgcagg atatccggca ggaggcgccg cgtgaggctg ggctgcaggc cgttggtgga
     2281 gatggtcatg gcggccttcg gggccgcctc cttgaccgcg gccaccacgt cgaccaggtc
     2341 gcgccggagg aaggtttccc caccggtgat gttcacgtgg gtgagggtgt ccgggatggt
     2401 ggcccggtat tcctcggggg tcatctcccc tttcgaagac tggtctcctt cccagatgcc
     2461 acacatgacg cagcgggagt tgcagcggta ggagacgacg agcagaaggt catcggggat
     2521 gaatttcagg ccggcggtgt cgaggtgcat cccagctccg ggtcatttgg cgcagccggc
     2581 ggccgggcac gcgcccgccc ccggcatcgg ggagaacatg atagcagagg ggccgcgagc
     2641 gccccggctc accttcgatt atcggccggg gcccggggat cccgagtgat cggggccccg
     2701 gcgcattttt tccacccggg ctctcatcat ctcgcgataa accgcctcga aacgatcgac
     2761 gaccgacggc caggcgtatt cccggagcac ccgctcgcgg gccagcccac ggaccccctc
     2821 cagccgctcc gggtgggcca ggaggtcacc cagcacgcgg gccaggtcgc ttgcgtcacc
     2881 cgagcggaag tggaagccgg ccccgcccac cccttcgagc atctcgggga tgtcgctggc
     2941 caggaccgga gctccctggc ccatcgcctc caggaggacg aggctcacgg catcccgctc
     3001 ggtgggattc acgaggagcc ggcaggccgc gtagagctcg gtgagctccg cgccgaggat
     3061 ggtgccgccg aaacgaaccg acggtcccgc cgaggccttg agtcgtgcca cgtagtcgtc
     3121 cgaatgggcc ccctcgccgc agacgacgat ggggagatcg agcttgagcc gacggtgcgc
     3181 ctcgatcagg tggtggcacc ccttctcggg aacgaggcgg ccgacgaaga ggagaaatcc
     3241 gtcgggaggc acgccgaggc gctcgaggat gccggtgggg cgcggttcgg gcacctccac
     3301 cgcgttcggg atgaaggttg actcgcggcc gaggcgggcc tggtagtcat cgcgcgaggc
     3361 cagcgtgttg acgacggtgc gttgggccgc ggccagcccg agccgctcgc agcgtcggaa
     3421 gtaatcctgc gccttcttcc cccactttcc ccctttccac tcctggccgt ggacatggaa
     3481 gaggacacgc gcgccggcgg cccgcagcag cggaatgacc ggcgcgtttc cgagcgagtg
     3541 gacatgccag atgtcgaaac cgcgcgggag gccgtcgagg acggaggcga aggcgtggct
     3601 caaggtgtcc gaatacttgc cgggcagcga cggcgtgacc cgcacgcgga cgccgcggtg
     3661 acgctccgga gccgcgcttc ccacgtagcc gcgccgcgcg tagaccgaaa cttgatgtcc
     3721 gcgctcacca agacgcgccg agagctcctc gacgatgcgc tccacgccgc cgaccgtggc
     3781 cggaatcccc ttcgagccga tcatcgccac gcgaagcggg cggccgggcg tgctcacagt
     3841 ccgtagacgg acttcatcca ggccacggtc agcggaatgc cttcggccag cggcacctga
     3901 ggcgcgtggc cgaggtcgcg tcgcgagagc gtgagatcga ccttcttgtc cttcgtggtg
     3961 aacggctcgg cctcgacgta ttcgaccagc cggtcggtct ttccacatgc cttgaggatg
     4021 aggtcggaga gctgccggat gtcgtggtac tcctcgccgc cgatgttgta caccgcaccg
     4081 ggcttgaacc gctcggcgat gttggccagc gtgcggacgg tgtcgtccac ataggacgag
     4141 gtgcggtggt gattgaggta caccttgtac ggctggtcgg tgagggcgcg gtagatgaag
     4201 aggcagcaga cgctgcgata cgtactgtag tgctcgccgg gaccgtaggt gttgaagagg
     4261 cggacgcgca ccgtctcggt gccatgcatg gccgcggagt tcaggcactg ctgctcgccc
     4321 acccacttgg tcatggcgta gtcgttcagc tggcggatct cgaccttgtc catgacgtct
     4381 tccgccatca cgccgtcgta atcgccgtac acctcgctgg acgagaagaa gaccatccgg
     4441 aagcggtgcg cttcttgaag tcggatcagg ttcttggtgc cgaccacgtt cgagcgccag
     4501 agcgtgtcgt agaagtcctc tccgttccag cgcccgaact cggcggccag gtggtacacc
     4561 acgtcgaact ggcgcccctc gaagacccgc tcgatctgcc ggtagaggcc gacatcggcc
     4621 cggagatact gggcgtcgtg gtggtggggg aggtcgacga tccagacgtt gtttccgcga
     4681 cggcggagct cggccacgag gaccgaaccg accgtgccga ggccgccggt gaccaggatg
     4741 ttcatgtcgt gtggtgtgtc ctttcgttgg tcccggccgg tccgggaacg agctccttgc
     4801 ggagccgttt gccgatcacg gccacgtcgt aatcgcgccg ggcgcgttcc cggccggcct
     4861 gcccgagcct caaggcgcgg gccggatcgt ccaggagtcc cgcgatggca tctcccagcg
     4921 ccgtcacatc gcccggatcg acgagaacgg catcgacgcc atccgtgacg agttcgggaa
     4981 tgtcgccgac ccgggtgatc accaccggcc gcccactggc caggtactcg accagtttgg
     5041 tcggaacgcc ggcccggttc agcaggtggt cggccttggg cgccacgagc acgtcggccg
     5101 cccggaaaca ggacggcatc tcgagactgt cccgcacccc ggtaaaacgg atgcggtccg
     5161 ccgcgaccag cccggccacc gaatcccggc aacgctgcca ggcatcggcc ggatgccccc
     5221 ccaccaccac cagcgtgacg tcgggacgcc catcgaggaa ccggggcgcg gccgcgagga
     5281 gcgacgggac accttcgtta tcgaaaaatg cgccggcgta cagtaccacg cgcccttccg
     5341 gcatgccaaa acgccgtcgc cacggggcgt cggggtccgg gacatcgaag gcggccgtat
     5401 cgacgacggg gggaatgcgg cggagccgct cggggcgcac cgcttgcgcc atcgaagcga
     5461 acaggtagtg actgatgacg aaggtgcgat cgagacgggc cggcagccag gtcatcccga
     5521 gccggaaatt gagcgaccag agccgccgcg ccgggttggt ctcgatgcga tcgtggtcga
     5581 cgaacgacaa gtcgctgtag tcggcggaaa ccctggcgcc ggtaagtcgg gccaggagcc
     5641 agtaagccgc cccgtcgtag aacgtcggct ggtagagatg aaaattgcgg tacccggcga
     5701 tgcgaagtcg cacgagcgcc accagcgaca ggaagaggct gtaccagcgg agcaggaaga
     5761 gcctggccat tccggcggtg ccgatccccg gcgtgtgtcc gtacgatccc tgccgcgcca
     5821 ggcggatcac gtggatgccg tcgatgattt cgggaccttg cacttttgcg ggcggcggca
     5881 cgagtccgcg cgtgcagggg atcaccagcg cggtggtcat gccggcggcc acgaggctcc
     5941 gggccagcat gtgcacgcgc cgggtgtcgc caccaacgcc gttcgggaag tcgacgatgg
     6001 ccaccatcgc caccctgggg gcgctcaccg ggagggctcg tgccccgggg tgtcgtcgtg
     6061 gttggccacc agggcggcca tcacccagag cgggccga
//
LOCUS       DASVOD010000691         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_515173, whole genome shotgun sequence.
ACCESSION   DASVOD010000691 DASVOD010000000
VERSION     DASVOD010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515173"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VF720_14855"
     CDS             <1..514
                     /locus_tag="VF720_14855"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880693.1"
                     /translation="HRALGFTPVGVFHRVGWKFGRWHDTSWWERPLATGRPRLLFVCV
                     ENSNRSQMAEAFARLHGGDRVDAFSAGSKPSGRINPRAIEAMRELGYDLTTHRQRSLD
                     DIPPGEYAAVITMGCGDSCPFVPAARREDWQLADPRDMAPAEFRAVRDEIGARVRRLV
                     DEIVTPRQST"
     gene            645..1511
                     /locus_tag="VF720_14860"
     CDS             645..1511
                     /locus_tag="VF720_14860"
                     /inference="COORDINATES: protein motif:HMM:NF042826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX7880694.1"
                     /translation="MAAAATLLLLALPALAQQADTTAAAPAAAPFRFFSSERLNILLP
                     IVGFTMLILFFIQEARKGKSLFIRHIPGLDAIEEAIGRCTEMGRPILYVPGIDDATNI
                     QTIYSMIILNHVAKRVAQYDTPLIVPVGKAIVTPLAEEAVKSGYTAAGRPDAYNPNNI
                     RFMSDEQFAFTAAVNGIMNREKPAANIYLGSFFAESLVLAETGIMTGAIQIAGTANIH
                     QLPFFVVACDYALVGEEFYAATAYLSREPGLVGTLKATDVMKAVLMVTIIVGVLLEQF
                     GFHGFTAWMQPK"
     gene            1522..2172
                     /locus_tag="VF720_14865"
     CDS             1522..2172
                     /locus_tag="VF720_14865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880695.1"
                     /translation="MRREVPIMITIFIGWVMVLDFFFPPLSGTATHMRDWTLILIAVS
                     QMLGVANVARINLDKVSRRHQDAPYAVMLLVAMLVMITLGVVMPKSATIGTFTWGGIE
                     GGSLFDKVYNQMYVPMQGTMFSLLAFFIASAAYRAFKIRSPEATILAVTAVIIMIGQV
                     PIGSILWDGMPGLSAWIMNVPNLAAKRAILIGAALGAVATGMKVILGLERNYVGGE"
     gene            2176..>2703
                     /locus_tag="VF720_14870"
     CDS             2176..>2703
                     /locus_tag="VF720_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880696.1"
                     /translation="MNWMQRLEKLDRRIVFVIMFIAVSIPVLVRLDMPIPSSKEVDDY
                     YDAIEKLPDGSAVFFPADFDPGSVAELEPMMRTSLRHLFRKDCKVVCFTLWDTGPGIV
                     DAIIRDEAQRAHKEYGVDYVFLGFKAGREVIMVQIANSISGVFPQDYTGKDTKSLPLM
                     QQVDKLTDTRLIVNIS"
BASE COUNT          470 a          935 c          826 g          472 t
ORIGIN      
        1 gcaccgcgcc ctcggcttca cgcccgtcgg cgtgttccac cgggtcggct ggaaattcgg
       61 ccggtggcac gacacctcct ggtgggaacg gcccctcgcc accggccgtc cacgcctgct
      121 cttcgtctgc gtcgagaact cgaaccgcag ccagatggcc gaggcgttcg cgcggcttca
      181 cggcggcgat cgcgtcgacg ccttcagcgc cggctcgaag ccctccggtc gcatcaaccc
      241 cagggccatc gaggccatga gggagctcgg ttacgatctc acgacgcacc gccagcgctc
      301 gctcgacgac atcccgcccg gcgagtacgc cgccgtgatc acgatgggat gcggcgacag
      361 ctgtcccttc gtcccggccg cgcgacgcga agactggcaa ctggccgacc cgcgtgacat
      421 ggcgccggcg gagttccggg cggtgcggga tgagatcggg gcgcgtgtcc gacggctggt
      481 cgacgaaatc gtcaccccgc ggcaatcgac ttgacgcacg cgccacggac cattagcttt
      541 ccgcggtccg taacggtttg cccgttcggg acaactgttg acaaagcgag ggggattgga
      601 tggcggtacg gggaagaacc cggctcggcc cgataatggc atggttggca gccgccgcca
      661 ctctgttact cctggccctc cccgccctgg cccagcaggc cgataccacg gccgccgccc
      721 ccgccgccgc tcccttccgc ttcttcagtt ccgagcggct caacatcctc ctcccgatcg
      781 tcggcttcac gatgctgatc ctgttcttca tccaggaagc caggaagggg aagagcctct
      841 tcatccggca cattccgggc ctcgatgcca tcgaggaggc catcggccgc tgtacggaga
      901 tgggacggcc gatcctctac gtgccgggca tcgacgacgc cacgaacatc cagacgatct
      961 actcgatgat catcctgaac cacgtggcca agagggtggc gcagtacgac acgcccctca
     1021 tcgtgccggt cgggaaggcc atcgtgacgc cgctggccga ggaagcggtg aagagcggtt
     1081 acaccgccgc cggccggccc gacgcctaca atccgaacaa catccgcttc atgtccgacg
     1141 agcagttcgc cttcacggcg gccgtcaacg gcatcatgaa ccgcgagaag cccgccgcca
     1201 acatctacct gggctcgttc tttgccgaga gcctcgtgct ggcggaaacg ggcatcatga
     1261 ccggcgccat ccagatcgcc ggtacggcca acatccacca gcttccgttc ttcgtggtgg
     1321 cctgcgatta cgcgctggtg ggcgaggagt tctacgcggc cacggcgtac ctgagccgcg
     1381 agccgggcct ggtgggcacg ctcaaggcca ccgacgtcat gaaagcggtg ctgatggtca
     1441 cgatcatcgt cggcgtgctg ctcgagcagt tcggcttcca cggcttcacg gcctggatgc
     1501 agccgaagta aggggagagg gatgcgccgc gaagtaccca tcatgatcac catcttcatc
     1561 ggctgggtca tggtgcttga tttcttcttt ccgcccctct ccggcaccgc cacgcacatg
     1621 cgcgactgga cgctcatcct gatcgcggtc agccagatgc tgggcgtggc caacgtggcc
     1681 cggatcaacc tggacaaggt gagccgccgt caccaggacg cgccctacgc ggtcatgctt
     1741 ctggtggcca tgctcgtcat gatcactctc ggcgtggtca tgccgaagtc ggccacgatc
     1801 ggcacgttca cctggggcgg catcgaaggg gggagcctct tcgacaaggt gtacaaccag
     1861 atgtacgtgc cgatgcaggg aaccatgttc tcgctgctgg ccttcttcat cgcctcggcc
     1921 gcctatcgtg ccttcaagat ccgcagcccc gaagccacca tcctggccgt cacggccgtg
     1981 atcatcatga tcggccaggt gccgatcgga tcgatcctct gggacggcat gcccggcctc
     2041 tcggcctgga tcatgaacgt gcccaacctg gcggccaagc gcgccatcct gatcggcgcc
     2101 gccctcgggg ccgtggccac cggcatgaag gtcatcctcg gcctcgagcg caattacgtg
     2161 ggaggcgagt agtccatgaa ctggatgcag cgcctcgaaa agctcgatcg ccggatcgtc
     2221 ttcgtcatca tgttcatcgc cgtgtcgatc ccggtgctcg tgcgcctcga catgccgatc
     2281 ccgtccagca aggaagtgga cgactactac gacgccatcg agaagctccc cgacggctcc
     2341 gcggtgttct ttccggccga cttcgacccg ggcagcgtgg ccgagctcga gccgatgatg
     2401 cgcaccagcc tccgccacct cttccgcaag gactgcaagg tggtctgctt caccctgtgg
     2461 gacaccggcc ccggcatcgt ggacgccatc atccgcgacg aggcccagcg cgcccacaag
     2521 gaatatggtg tggactacgt gttcctcggc ttcaaggccg gccgcgaagt catcatggtg
     2581 cagatcgcca actcgatcag cggcgtgttc ccccaggatt acaccggcaa ggacaccaag
     2641 tcgcttccgt tgatgcagca ggtggacaag ctcaccgaca ccaggctcat cgtgaacatc
     2701 tcg
//
LOCUS       DASVOD010000692         5884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_828031, whole genome shotgun sequence.
ACCESSION   DASVOD010000692 DASVOD010000000
VERSION     DASVOD010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828031"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="VF720_14875"
     CDS             complement(<1..836)
                     /locus_tag="VF720_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880697.1"
                     /translation="MKASRVLLPALLVLLGTSLAFPAGASFHLMQIEQVIGGVNGDVT
                     AQAIQLRMRAAGQNLVGQSRIKVRDAAGANPITLIDMTTNVPNGVTGDRVLLVTAPFQ
                     TYVNAAVTPDFVFTTPIPASYLAAGSLTFEDDFGTIYWRVSWGGAGYTGTGSMSSLND
                     PNLNCNPAFAGALPSTTLQALKFQGASTAQSNTNGSDYALTPGASVWTNNARQNETLE
                     SQTGVGDPPRPGTARIELSRAFPNPTRGASHVTFVLESAGHAVVSVYDAVGRRVRVLL
                     DGS"
     gene            complement(992..1066)
                     /locus_tag="VF720_14880"
     tRNA            complement(992..1066)
                     /locus_tag="VF720_14880"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1031..1033),aa:Glu,seq:ctc)
     gene            complement(1118..1191)
                     /locus_tag="VF720_14885"
     tRNA            complement(1118..1191)
                     /locus_tag="VF720_14885"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1157..1159),aa:Gln,seq:ctg)
     gene            complement(1338..2816)
                     /gene="gltX"
                     /locus_tag="VF720_14890"
     CDS             complement(1338..2816)
                     /gene="gltX"
                     /locus_tag="VF720_14890"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX7880698.1"
                     /translation="MSRPVRVRFAPSPTGYLHVGGARTALFNWLYARRHGGTFILRIE
                     DTDAERSTEESTKAIYEGMRWLGLTWDEGPDIGGPHAPYVQTERKAIYHAIAGELVAK
                     GLAYPCFCTPDELKERRELAMKEGRTPGYDGRCRNLSTDQIEERKGAGLPMALRIRTP
                     PGESSWVDAIKGEITFDHRNLEDFVILKSDGNPTYNFAASVDDIRMEITHVLRGDDHV
                     SNTPRQIILYRALGAELPVFGHLPMILGGDGSRLSKRHGATAVDAYADMGFVPDAMIN
                     YLALLGWSLDGEREVFSRQDLIANFSLERCGSNASVFDIQKLEWMNGLYLREMPVAER
                     TALVTGYLAERGLWPRPGRDEAWLNHVVDAVGDRLKRINSILDYAGFLFTDEVEFDPV
                     AFDDMVKRKRVGEILPAMREMLARVEPFDLATTEAELRKTAEGLGLKAGDLIHPTRVA
                     LSGKKVGPGIFEVIVLTGREEAVKRLDRAERLVAEAPAPASS"
     gene            complement(2840..>5884)
                     /locus_tag="VF720_14895"
     CDS             complement(2840..>5884)
                     /locus_tag="VF720_14895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7880699.1"
                     /translation="RAGELFATGTTDGAGLVMLDCPAENPGPVSLVVTARNSLPHEGS
                     LTLTLPGSAAWPTIRGIRVDDALLGNGNGRLESGETADLYLTIRNDGPGAANALGVSI
                     SSATAGISFPTAAAVLGSLSSGATDSTWEGSPLRVAVVSGAPRLLDVESVVTLDWAGG
                     SRTIPVGLRVGGPVILVRSQSFVDDPGGPGTNGLVAPDELVHLTPVISNRGAARSASV
                     VVRLESVDPRVTLVNAESVVGPLDPGQALPVPAPLAFRVSELPALATIDVVVTVGGFE
                     VSRRAVDLLAPAVPPVAFGLSRPSSILLTWHPVPDADTRGYAVYRATSEAGPYTLLNP
                     DADNDMGYYEDVDLPPFSRWYYRIAAIDRSGNESGVTPTVNFATSYPTLGNWPIRREG
                     ATPSSPTVHDFDGDGTLEIVIGGEEVYAVRADGTEYLDGDDDSRTLGILTRTGGANFW
                     TTPAVADVDADGVLDIVAAGFNDGKLYVFGSDGAIKPGWPQALGNGINQSGAAVPWGS
                     PLIADLTGDGPCEIFIAGDRFLYAFHADGTEVSDGDSNPATYGPWRDLGGAFNYATPS
                     AADFDGDGRPEIVMGTRAGQLLLIDDTGALMPGFPKTFTGGQITGSAAIADLDKDGEL
                     EMVFPVRNLAAVHAVNRFGASPPGWPAPIQINQDIDASPTIADLDGDTFPDVVIVGGA
                     GRVIIVHGQNCTLFPGFPVNLANQEQAQSFATRGTPCLGDVTGDGMPDILVGTQTASV
                     YGVGINGQVLPGFPLRAEDNVEGGPLLWDVDGDGHTNIVFGSVDRKVYAYDTPGIFNP
                     TSCPWPMFRHDQGNTGYVGSPLTTPARPVLASFTAAVTNGTVQLSWRAPGSEWTAWLV
                     DRAPVGEPDHFTRLTTEALTHGTGDMTFVDTAIVPGERYRYRLTALDRSGALVAFAPL
                     DVEVPVEAPRALVAAVSPNPFREGTTLSYAVGAPGRVTFDVFDAQGRRIRRLESIDRP
                     AGQYSVAWDGRDDSGAPAESGVYYWRLTTGSGSMKGKLTRIP"
BASE COUNT          959 a         1968 c         2029 g          928 t
ORIGIN      
        1 gatccgtcga ggagcacgcg cacccgtcgt cccacggcat cgtagaccga aacgacggcg
       61 tgcccggccg attcaagcac aaacgtcacg tgcgacgcgc cgcgggtcgg gttcgggaac
      121 gcccgggaaa gctcgatgcg ggcggtgccg gggcgcggcg ggtcgccgac gccggtctgg
      181 gactccagcg tctcgttctg ccgggcgttg ttcgtccaga cggaggcgcc cggcgtcagc
      241 gcgtaatcgg agccgttggt gttgctctgg gccgttgaag cgccctggaa cttgagggcc
      301 tgcaaggtcg tggacggcag tgcgcccgcg aatgccggat tgcagttcaa gttggggtcg
      361 ttgagcgagc tcatgctccc cgtgccggta tagccggcgc cgccccagct gacccgccag
      421 tagatggtgc cgaaatcgtc ctcgaacgta agcgaaccgg cggccaggta cgacgccgga
      481 atcggcgtcg tgaacacgaa gtcgggcgtc acggccgcat tgacgtacgt ctggaacggg
      541 gctgtcacga gcagcacccg gtcaccggtc acgccgttgg gcacgttcgt ggtcatgtcg
      601 atcagcgtga tcgggttggc tcccgccgcg tcacgcacct tgatgcgcga ctgccccacc
      661 aggttctgcc cggcggcacg catgcgcaac tggatggcct gggcagtgac atcgccattg
      721 acgccgccga tgacctgctc gatctgcatg agatggaagc tggcaccggc ggggaatgca
      781 agactcgtcc cgagcaggac cagaagggcc ggaagcaaca cgcgcgatgc cttcattggg
      841 caaacctccg tgggactggc gagctggcgg cgggttgacg ggccctgtac cggagtcggg
      901 accgacgaga cgagtttaag ccccggcggg ggcgtttgcc gcggggaact tgaaaaaatg
      961 gggtaccgac tttcgtcgat accccggctt gtggtagcgc tacggggatt cgaaccccgg
     1021 tttgatggct gagaaccacc cgtcctgacc cctagacgat agcgccgtat cggttggcgg
     1081 cagcgcgcgg gtcgggcgcc ttgccgatca tgctgagtgg ctggggagca gggattcgaa
     1141 ccccaattct ctgctccaga ggcagatgtc ctaccgttga acgactcccc aacttattac
     1201 atttgaagcg gttacgccgt tcaccgactt tgtcggcgtc aggacggcag ggaatttacc
     1261 agccacggca tgagctatgc aaccctatta atagcgtcgg tcggcccggt cggcagcgcg
     1321 cctggcacgt cggagggtca cgaagaggcc ggcgccgggg cctccgcaac cagcctctcg
     1381 gcccggtcga gccgcttcac cgcttcctcg cgccccgtca ggacgatcac ctcgaagatc
     1441 cccggcccga ccttcttgcc ggagagcgcc actcgggtcg gatggatgag gtcgcctgcc
     1501 ttgagtccaa gtccctcggc ggttttcctg agttcggcct cggtcgtggc caggtcgaac
     1561 ggctcgacgc gggccagcat ctcccgcatg gccggcagga tctcccccac ccgcttccgc
     1621 ttgaccatgt cgtcgaaggc cacgggatcg aactcgacct cgtccgtgaa gaggaaaccg
     1681 gcgtaatcga ggatgctgtt gatccgcttc aggcgatcgc cgacggcatc gacgacgtgg
     1741 ttgagccacg cttcgtcgcg gccggggcgc ggccagagac cccgctccgc gaggtagccg
     1801 gtcacgagag cggtccgctc ggccaccggc atctcgcgca ggtaaaggcc gttcatccac
     1861 tcgagcttct ggatgtcgaa gaccgaggcg ttgctgccgc aacgctcgag cgagaaattg
     1921 gcgatgagat cctgccggct gaagacctcc cgctcgccgt cgagcgacca gccgaggagt
     1981 gccaggtagt tgatcatggc gtccggcacg aaacccatgt cggcgtaggc atccacggcg
     2041 gtggcgccgt gccgcttgga aagccggctc ccgtcgccgc ccaggatcat cggcagatgg
     2101 ccgaagaccg ggagctcggc gccgagcgcg cgatacagga tgatctggcg gggtgtgttc
     2161 gacacatggt cgtcgccccg caagacatgg gtgatctcca tccggatgtc gtcgacggag
     2221 gcggcgaagt tgtaggtggg attgccgtcg gacttcagga tgacgaaatc ctcgaggttc
     2281 ctgtgatcga aggtgatctc ccccttgatc gcatccaccc acgagctctc gcccggcggt
     2341 gtgcggatgc gaagcgccat cggcagcccc gcccccttcc tctcctcgat ctggtcggtc
     2401 gagagattcc ggcaacggcc gtcgtagccc ggcgtccgcc cctccttcat ggccagctct
     2461 cgacgctcct tcaattcgtc cggcgtgcag aagcagggat acgccagccc cttcgccacc
     2521 agctcgccgg cgatggcgtg gtagatggct ttccgctccg tctgcacgta gggtgcgtgc
     2581 gggccaccga tgtcgggacc ttcgtcccag gtgaggccca gccagcgcat cccttcgtag
     2641 atggccttcg tcgactcctc ggtggagcgc tcggcgtcgg tatcctcgat gcgcaggatg
     2701 aacgtaccgc cgtggcggcg cgcatagagc cagttgaaca gggccgtgcg ggcacctccg
     2761 acatggagat agccggtggg gctcggggcg aaacggacac ggacaggtcg ggacatcgct
     2821 tctccggaag tcggggcggc tatggaatgc gggtcagctt gcccttcatg gagccgctgc
     2881 cggtggtcag ccgccaataa tagacgcccg actcggccgg cgcacccgag tcgtcgcgac
     2941 cgtcccaggc cacgctgtat tggccggccg gacgatcgat cgattcgagc cggcggatcc
     3001 ggcgcccctg ggcgtcgaac acgtcgaacg tcacgcgccc cggggcgccc accgcgtagc
     3061 tgagcgtggt gccctcgcgg aacggattgg gcgagaccgc cgccaccagc gcccggggtg
     3121 cctcgaccgg cacctccacg tcgagtggcg cgaaggccac gagggcgccc gatcgatcga
     3181 gcgcggtcag gcgatagcga taccgctcgc ccggaacgat ggcggtgtcg acgaacgtca
     3241 tgtccccggt gccgtgcgtc agggcctcgg tcgtgagccg cgtgaaatga tccggctcgc
     3301 cgaccggcgc acgatcgacc agccaggcgg tccattcgct gccgggcgcc cgccacgaaa
     3361 gctggacggt cccgttggtg acggccgcgg tgaaggatgc gaggaccggc cgcgccggcg
     3421 tggtcaacgg gctccccacg tatcccgtgt tcccctggtc atgccggaac atcggccatg
     3481 ggcaactcgt gggattgaaa ataccgggcg tgtcataggc gtacaccttg cggtcaaccg
     3541 acccgaaaac gatgttggtg tgcccgtcgc cgtccacgtc ccagagaagc ggcccaccct
     3601 ccacgttgtc ctcggctcgc agcgggaagc ccggaagcac ctgcccgttg atccccacgc
     3661 cgtagacgct ggcggtctgc gtgccgacca ggatgtcggg catgccgtcg ccggtcacgt
     3721 cgccgaggca gggcgtgccg cgcgtcgcga acgactgggc ctgctcctgg ttggccaggt
     3781 tcaccgggaa gcccgggaag agcgtgcaat tctggccgtg cacgatgatg acgcgacccg
     3841 ccccgcccac gatcaccacg tcggggaacg tgtccccgtc gaggtcggcg atggtcggcg
     3901 aggcatcgat gtcctggttg atctggatcg gggccggcca gccgggcggc gaggcgccga
     3961 accggttcac cgcatgcacc gcggccaggt tgcggaccgg gaagaccatc tcgagctccc
     4021 cgtccttgtc caggtcggcg atggcggccg aaccggtgat ctggccgccc gtgaacgtct
     4081 tcgggaaacc gggcatcagg gcgccggtgt cgtcgatcag gaggagctgg ccggcccgcg
     4141 tccccatcac gatttcggga cgaccatccc cgtcgaagtc ggcagccgaa ggagtggcgt
     4201 agttgaacgc acctcccagg tcgcgccatg ggccgtaagt ggccgggttg ctgtcgccgt
     4261 ccgacacctc ggtgccgtcc gcatggaagg cgtacaggaa ccggtctccc gcgatgaaga
     4321 tctcgcaggg gccgtctccg gtcaggtcgg caatgagggg cgatccccat ggcacagccg
     4381 cgccggactg gttgatgccg ttgcccaggg cctgcggcca gccgggcttg atggcgccgt
     4441 cgctcccgaa gacgtaaagc ttcccgtcgt tgaagccggc ggccacgatg tcgaggacgc
     4501 cgtcggcatc cacgtcggct acggccggcg tggtccagaa gttggcgccc ccggtccggg
     4561 tcaggatgcc gagcgtgcgg ctgtcgtcat caccgtcgag gtactcggtg ccgtcggccc
     4621 gcaccgcgta tacttcctcg ccgccgatca cgatctccag cgtgccgtcg ccgtcgaagt
     4681 cgtgcacggt cggcgacgac ggcgtggccc cctcccggcg gatgggccag ttgccgagcg
     4741 tcgggtagct cgtggcgaaa ttcaccgtgg gcgtcacgcc ggactcgttg ccggagcgat
     4801 cgattgccgc aatccggtag taccagcggg agaacggcgg caggtcgacg tcctcgtagt
     4861 accccatgtc gttgtcggca tccggattca acaacgtgta cgggccggct tcggaggtgg
     4921 cccggtagac ggcgtagccg cgagtgtccg catcgggaac cgggtgccag gtcaggagga
     4981 tcgacgaggg gcgcgacagt ccgaacgcca ccggcggcac agcgggcgcc aggaggtcga
     5041 cggcgcggcg actcacttcg aagcccccga cggtcacgac cacgtcgatc gtcgccaggg
     5101 ccggcagttc gctgacgcgg aaagccaggg gggccgggac cggcagggcc tggcccgggt
     5161 cgagcgggcc cacgaccgac tcggcgttga cgagcgtcac ccggggatcg accgattcca
     5221 ggcgaaccac cacgctcgcc gagcgcgcgg cgccgcggtt gctgatcacg ggggtcaggt
     5281 ggaccagctc gtccggtgcc accaggccgt tggtgcccgg accaccggga tcgtccacga
     5341 acgactgcga gcgcaccagg atcacgggcc cgccaacacg cagccccacc ggaatcgtcc
     5401 gcgagcctcc ggcccagtcg agagtgacca cggactccac gtcgagcagg cgcggcgccc
     5461 cgctcacgac tgccacgcgc agtggtgatc cttcccaggt cgagtcggtg gccccggacg
     5521 aaagcgatcc gagcaccgcg gccgccgtcg ggaacgagat acccgcggtg gccgaggaaa
     5581 tcgatacgcc gagcgcgttg gccgcgccgg gaccgtcatt gcggatcgtg agatagaggt
     5641 cggccgtttc gccgctttcc aggcgcccgt tgccgttacc caggagcgca tcatccaccc
     5701 ggatgccgcg aatggtgggc cacgccgccg aacccggcag cgtcagcgtc aacgacccct
     5761 cgtggggcaa cgaattgcgt gccgtcacca ccagcgagac cggcccgggg ttctcggccg
     5821 gacaatccag catcaccagg cccgccccgt ccgtggtgcc ggtggcgaag agctcgccgg
     5881 cccg
//
LOCUS       DASVOD010000693         7963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_409399, whole genome shotgun sequence.
ACCESSION   DASVOD010000693 DASVOD010000000
VERSION     DASVOD010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409399"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..2344)
                     /locus_tag="VF720_14900"
     CDS             complement(8..2344)
                     /locus_tag="VF720_14900"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880700.1"
                     /translation="MTRPAWTENAKTGGTVLERRFMLSMAVVILALMGPAMVVVQFRA
                     AEHFQESAQVRGASMARSIAAVATPALLAYNYVGLEDAARGASQDEGVAYVIIHDKEG
                     LVAGDSRRRDYEARRSRDPISSAAVQAPEVLVQRARYSGPDGPIDVLDVAVPVYVANS
                     SEKWGTVRVGISLEPVRQAVGRITWSLGALGLLGFVLCLLGARSASRKITAPLQTLRE
                     GTMALAQGDLTHRIDIATGDEIEDLAGHFNHMADEIAARAAEARDARGALENLNASLE
                     QEVVQRTAALAGSESRYRALVEGAPLGIAIVQGGRVVYSNPAYQRLTAGDSRNPFELL
                     DDEERTTLLAEMSAWTRAEVFGPHEVRLVTKDNPARFATMRWMAVELEGEAADLCLLE
                     DVTAVRKLQEQVVVSDKLRALGELASGVAHDFNNCLAIILGRCQLLALRSKDPDLLKG
                     LAIIEKAASDGGQTVRRIQDFARMRKETRRESIDMKELIEDVVEITRSKWKNEAQGKG
                     LSIEVDTEFAHTASIDGNAAELREALTNLIINAVDAMPRGGRLSFRTCDERVEERDMV
                     RVEVTDTGQGIPHEVQAQIFDPFFSTKGNLGTGLGLSVTYGIVTRHGGLIAVESVPDE
                     GTTFVLRLPVGTAAPGSSEAAADEEIPFVPATVLVVDDEPEIRDVLVEGLTAAGYKVT
                     PVGSGREAITRLGLVHYDVVLTDLGMPEVTGWDVVAAARTTRPEMILGLVTGWGETLD
                     PAQVAERGVTFVIAKPFDVRRVVGDIRKSVLNRSRHAA"
     gene            complement(2322..3995)
                     /locus_tag="VF720_14905"
     CDS             complement(2322..3995)
                     /locus_tag="VF720_14905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX7880701.1"
                     /translation="MTQIAHNTPAPARARGWSRFALSIVLLILALTPLTSAHSRTAAL
                     VTVQPKRGGTLRFVQEQVSTLDPLPLGDVYSATVAAQIHRGLLFYGTNLTPMPDLAES
                     WTISRDGLDYVFRLRQGARFHNGRDVTMKDVIFTFERTFAPGGDPGLAGPFLSVIDGA
                     EEFAAGKATRIKGITALSDRNLRIHLKRPDAHFLWALAMTPAAVVPKAEFLAMGEAEF
                     ARHPIGCGPFRLLSSTDKALTLVAWRNYYRRAAWLDTLVFVTATASDHAATVKGLLSN
                     QYDIAEVPGLHREKVARSSTVRLVSRRELALSFLGLNVRLEPFTDPKVRQALALGVDR
                     EAILRLNPSGQVVATGVLPPGMSCYSPDDKTFPFDIAKARQKLAEAGHPGGKGLKPIP
                     YYTSPGSARNHAVDSMLVANWRLAGLPVTPVTITWVELQRRIDAFDAPLFSLSWVADI
                     PDPDSFVGSLFESTSPSNYSRYRNAEVDSLIARGRTTVDPRARQTIYDRVERLVVNDA
                     PVVPLYTTTSAYGVRRGVYGLELTPMGISCVDLANVWIGELEDDQARLD"
     gene            complement(4096..5673)
                     /locus_tag="VF720_14910"
     CDS             complement(4096..5673)
                     /locus_tag="VF720_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX7880702.1"
                     /translation="MAGPPKERRTSSDIVVKPVYAPGDPAGFDPKRELGAPGEFPYTR
                     GVHPGMYRTRAWTMRQYAGFGDAGETNRRFRFLLDQGQTGLSVAFDLPTQMGYDSDAP
                     MAVGEVGRVGVAIDTLADMETLLDGIPLEAVSTSMTINATAGILLAMYMAVGEKQGVP
                     AAKLSGTIQNDILKEYIARGTYIFPPLPSMRITTDIFEFARDNVPNWNTISISGYHIR
                     EAGSTAAQELAYTLLDGLTYVKAAVDRGLDVDSFAPRLSFFFNAHSNLFEEVAKFRAA
                     RRLWARLMKERFDPKKADSLKLRFHTQTAGSTLTAQQPMNNVVRTTIEALSAVLGGTQ
                     SLHTNSMDEALGLPSESAALLALRTQQILAEESGVADTVDPLAGSWFVESLTTDLENA
                     ARTLIDDVESRGGMLRAIETGYVQEEIHKSAWRAQQAVERGEQVIVGVNRYAMDEPPP
                     PTFRIDPALEPAQVARLAQVRARRDAPRVTAALSALETAARGSANLMPHILEAVRAYA
                     GVGEISDTLRRVFGTHD"
     gene            complement(5681..6220)
                     /locus_tag="VF720_14915"
     CDS             complement(5681..6220)
                     /locus_tag="VF720_14915"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX7880703.1"
                     /translation="MKATYVVQAGNRRVTVDIREVGDRYELTIDGTTHVVDSRRVGRT
                     AVRSLIIAGKSYEANTVQDGDKCDVYLNGDLFSLEVFDELWARAKEGKSDGAARGETI
                     QAPIPGAVVKLMVEAGQVVAAGQPVAVLEAMKMQNELTATWGGTVTDVKVKLGDTVAA
                     GAALIVVAPPVAEAAAAGN"
     gene            complement(6217..7731)
                     /gene="accC"
                     /locus_tag="VF720_14920"
     CDS             complement(6217..7731)
                     /gene="accC"
                     /locus_tag="VF720_14920"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX7880704.1"
                     /translation="MSRPFKKVLVANRGEIAVRVMRALRELGIPSVAVYSEADRLALH
                     VRMADEAWPVGPAPSRESYLVAENVLAAAKASGADAVHPGYGFLSENADFARKCREAG
                     VTFIGPPPEAIEMMGNKVVAREAARKAGAPVVPGSDGPVADEVTAVRVATEIGYPVML
                     KAAAGGGGKGMRIVRSEPELRSALSLVKGEAASAFGDDEVYLEKYIERPRHIEVQVLF
                     DGKGNGVHLGERECTIQRRHQKVVEEAPSPVITPHQREEMGEAALAIARAAGYVNAGT
                     IEFIYAPDGKFYFLEMNTRLQVEHPVTELVYDVDLVKEQIHIAGGGSLRLNQIDLIPR
                     GWAIECRIYAEDPYRGFLPVTGTIVRLRMPSGPGVRNDIGFYQGYEVPIYYDPMLAKL
                     SVSGQDREEARRRMLRALAEYQIDGLKTTIPFHRWLLQRPEFQKAEFDTGTIDRDFKG
                     LTPEVNPEAETAAMIAAVIHLHEGNTAGTTTGTNGDGEKPSAWKMAGRTGRWWR"
BASE COUNT         1181 a         2648 c         2809 g         1325 t
ORIGIN      
        1 ccggatgtta cgcggcgtgc cgggagcggt tgagcaccga cttgcggatg tcgccgacca
       61 cgcggcgcac gtcgaacggc ttggcgatga cgaacgtgac gccgcgctcg gccacctggg
      121 ccgggtcgag cgtctcgccc cagccggtaa cgagccccag gatcatttcg gggcgggtgg
      181 tgcgggcggc ggccaccacg tcccagccgg tgacctcggg cattcccaga tcggtgagca
      241 ccacgtcgta gtggacgagg cccaggcggg tgatggcctc cctccccgac ccgacggggg
      301 tgaccttgta gccagcggcg gtcaggccct ccacgagcac gtcgcgaatt tccggctcgt
      361 catccaccac gaggaccgtg gccggcacga aggggatttc ctcgtcggcg gccgcttcgc
      421 tcgagccggg cgcggcggtg ccgaccggca ggcgcagcac gaaggtggtc ccttcgtccg
      481 gcacgctctc cacggcgatg aggccgccgt ggcgggtgac gatgccgtag gtgaccgaga
      541 ggccgaggcc cgtgccgagg ttccccttgg tgctgaagaa ggggtcgaag atctgggcct
      601 gcacctcgtg cgggattccc tggccggtgt cggtgacttc cacgcgcacc atgtcgcgct
      661 cctcgacacg ctcatcgcac gtgcggaagg agaggcggcc gcctctcggc atggcatcca
      721 ccgcgttgat gatcaggttg gtcagcgcct cgcgcagctc ggcggcattg ccatcgatgg
      781 aagccgtgtg ggcgaattcc gtatcgacct cgatcgacag ccccttcccc tgggcctcgt
      841 tcttccactt gctgcgggtg atctcgacca cgtcctcgat cagttccttc atgtcgatcg
      901 actcgcggcg cgtctccttg cgcatgcggg cgaagtcctg gatacggcgg accgtctgcc
      961 ctccgtcaga ggcggccttc tcgatgatgg ccagcccctt gaggagatcg ggatccttgc
     1021 tgcggagcgc caggagctgg catcgcccga ggatgatggc caggcagttg ttgaagtcat
     1081 gcgccacgcc gctggccagc tcgccgagcg cgcgcagctt gtcggacacc accacctgct
     1141 cctgcagctt gcgcacggcg gtgacgtcct cgagcaggca gaggtcggcg gcctctccct
     1201 cgagctccac cgccatccag cgcatggtgg cgaagcgggc cgggttgtcc ttcgtgacga
     1261 ggcggacttc gtgcgggccg aacacctcgg cgcgcgtcca ggccgacatc tcggccagga
     1321 gcgtcgtgcg ctcctcgtcg tcgaggagtt cgaaagggtt cctggagtcg ccggcggtca
     1381 ggcgctggta ggccgggttg ctgtagacga cgcgcccgcc ctgcacgatg gcgatgccga
     1441 gcggcgcgcc ttccacgagc gcgcggtaac gcgattcgga gccggccagg gcggcggtgc
     1501 gctgcaccac ttcctgctcg agcgaggcgt tcaggttctc gagggcgccg cgggcatcgc
     1561 gcgcttcggc ggcgcgggcg gcgatttcgt cggccatgtg attgaagtgg ccggccaggt
     1621 cctcgatttc gtcgccggtg gcgatatcga tgcggtgcgt gaggtctccc tgcgccagcg
     1681 ccatcgttcc ttcgcggagc gtctggagcg gcgccgtgat cttgcgggag gccgagcggg
     1741 cgcccaggag gcagaggacg aagccgagga gacccagggc accgaggctc cacgtgatgc
     1801 ggcccaccgc ctggcgaacc ggctcgagcg agatgccgac gcgaaccgtg ccccacttct
     1861 cggaggaatt ggccacgtaa accggcaccg ccacgtcgag cacgtcgatc ggcccgtcgg
     1921 ggcccgagta acgggcgcgt tgcaccagca cttcgggcgc ctgcaccgcc gcggatgaaa
     1981 tcggatcgcg gctgcgtcgg gcctcgtagt cacgccggcg actgtcgccg gccaccagcc
     2041 cttccttgtc gtggatgatg acgtacgcca cgccctcgtc ctggctggcg ccgcgggcgg
     2101 catcctcgag gcccacgtag ttgtaggcca ggagcgccgg ggtggccacg gccgcgatgg
     2161 aacgggccat cgaggcgccg cgcacctgcg ccgattcctg gaaatgttcg gccgcccgga
     2221 actgcacgac gaccatcgcc ggccccatga gggccaggat gaccacggcc atcgagagca
     2281 tgaaacgcct ctcgaggacg gtgccgccgg tcttggcgtt ctcagtccag gcgggcctgg
     2341 tcatcctcga gctctccgat ccagacgttg gccaggtcga cgcacgaaat tcccatcggc
     2401 gtcagttcga ggccgtacac gccacggcgg acgccgtagg ccgaggtggt cgtgtacagc
     2461 ggcacgaccg gcgcgtcgtt caccacgagg cgttcgacgc ggtcgtaaat ggtctgacgg
     2521 gcgcgcgggt cgaccgtggt acgcccgcgg gcgatcagcg agtcgacttc cgcgttccgg
     2581 taacgcgagt agttgctggg cgacgtcgat tcgaagagcg agccgacgaa cgagtcgggg
     2641 tcggggatgt cggccaccca cgagagggag aagaggggcg cgtcgaacgc gtcgatgcgc
     2701 cgctgaagct cgacccaggt gatcgtgacc ggcgtgaccg gcaacccggc cagccgccag
     2761 ttggccacga gcatcgaatc cacggcgtga ttgcgcgccg agccgggcga tgtgtagtac
     2821 ggaatcggct tgagcccctt gccgcccgga tggccggcct cggccagctt ctgccgggcc
     2881 ttggcgatgt cgaacggaaa ggtcttgtcg tccggcgaat agcagctcat ccccgggggg
     2941 agaacgccgg tagccaccac ctggcccgag gggttcaacc ggaggatggc ttcccggtcg
     3001 acgccgagcg cgagggcctg ccggactttt ggatcggtga agggttcgag ccgcacgttg
     3061 agcccgagga acgagagcgc cagctcgcga cggctgacga gccgcaccgt gctcgacctg
     3121 gccacctttt cgcggtgaag gcccgggact tccgcgatgt cgtactggtt cgagaggagc
     3181 cccttcaccg tggccgcgtg gtcgctggcc gtggccgtga cgaagaccag cgtgtcgagc
     3241 caggcggcac ggcggtagta gtttcgccat gccaccagcg tgagcgcctt gtcggtgctc
     3301 gagagaagac ggaacgggcc gcagccgatg ggatggcggg cgaattcggc ctcgcccatg
     3361 gccaggaact cggccttcgg cacgacggcc gccggcgtca tggccagcgc ccagaggaaa
     3421 tgcgcgtcgg ggcgcttcag gtggatgcgc agattgcggt cggaaagagc cgtgatcccc
     3481 ttgatccggg tggccttgcc cgccgcgaac tcctcggcgc cgtcgatgac ggaaaggaac
     3541 gggccggcca ggcccggatc gccgcccggc gcgaacgtcc gctcgaaggt gaagatcacg
     3601 tccttcatcg tgacgtcgcg gccgttgtgg aacctggctc cctggcggag gcggaagacg
     3661 tagtcgaggc cgtcgcgcga gatggtccag ctctcggcga ggtcgggcat cggcgtcaga
     3721 ttggtgccgt agaagagcag gccgcgatgg atctgcgcgg ccacggtggc ggagtacaca
     3781 tcgcccagcg ggagtgggtc gagcgtcgac acctgctcct gcacgaagcg gagcgtcccg
     3841 ccgcgcttcg gctgcacggt caccagggcg gccgtgcggg agtgagccga tgtcagtggt
     3901 gtcagggcaa gaatgagcag cacgatcgac agcgcgaagc gtgaccatcc gcgcgcccgc
     3961 gctggcgccg gggtgttgtg ggcgatttgc gtcatcggcc cggttatcgg cactaactga
     4021 cggcggattc agtattttcg ggcgttgcga aacgacacga cggaggtcgg aggggtgctg
     4081 gcggggggcg gaaagtcagt cgtgggtgcc gaaaacccgc cggagggtgt ccgaaatttc
     4141 cccgaccccg gcataggccc ggaccgcttc gaggatgtgg ggcatgaggt tggcgctccc
     4201 ccgggccgcc gtttcgaggg ccgagagggc ggccgtcacg cggggggcat cccgccgggc
     4261 gcggacctgg gccagccggg ccacctgggc cggttcgagg gcggggtcga tccggaaggt
     4321 ggggggtggg ggctcgtcca tggcgtagcg gttgaccccc acgatgacct gctcgccccg
     4381 ttccaccgcc tgttgggcgc gccaggccga cttgtggatc tcctcctgca cgtagccggt
     4441 ctcgatggcg cgcaacatcc cgccgcgcga ttcgacatcg tcgatgaggg ttcgagccgc
     4501 gttctcgagg tcggtggtaa ggctctcgac gaaccacgag ccggccagcg gatcgaccgt
     4561 gtccgccacg cccgactctt cggccaggat ctgctgtgtg cggagcgcga ggagcgccgc
     4621 cgactcactc gggagtccga gggcttcgtc catcgaattc gtgtggaggc tctgggtgcc
     4681 gccaagcact gccgacagcg cctcgatcgt ggtgcgcacc acgttgttca tcggctgctg
     4741 agcggtgagt gtggagccgg cggtctgcgt gtggaagcgg agcttgagcg agtcggcctt
     4801 cttcgggtcg aagcgctcct tcatgagtcg cgcccagagg cgccgggccg cccggaactt
     4861 ggccacttcc tcgaataagt tcgagtgcgc gttgaaaaag aacgagaggc gcggcgcgaa
     4921 cgaatcgaca tcgaggccgc ggtcgacggc cgccttcaca tacgtgaggc cgtcgaggag
     4981 tgtataggcc agctcctgcg cggccgtcga gccggcttcg cggatgtggt agccggaaat
     5041 ggaaatggtg ttccagttcg gcacgttgtc gcgcgcgaac tcgaagatgt cggtggtgat
     5101 ccgcatcgac ggcagcggtg ggaagatgta ggtgccgcga gcgatgtatt ccttcaggat
     5161 gtcgttctgg atggtgcccg acagcttcgc ggccggcacg ccctgcttct cgccgacggc
     5221 catgtacatg gcgaggagaa tgccggcggt ggcgttgatg gtcatcgacg tcgacaccgc
     5281 ctcgagcggg atgccgtcga gaagcgtctc catatcggcc agcgtgtcga tggccacgcc
     5341 cacgcgcccg acttcgccca ccgccatcgg ggcatcgctg tcgtatccca tctgcgtggg
     5401 gaggtcgaag gccacggaca ggccggtctg tccctggtcg aggaggaagc ggaagcggcg
     5461 gttcgtttcg ccggcgtcac cgaagccggc gtactggcgc atcgtccagg cgcgggtgcg
     5521 gtacatgccg ggatggacgc cgcgcgtgta ggggaattcg ccgggcgcgc ccagctccct
     5581 cttcggatcg aaaccggccg gatcgcccgg tgcgtaaacg ggcttcacga caatgtcgga
     5641 tgaggtgcgt cgttccttcg gcgggccggc cacggctgcc tcagttcccg gcggcggccg
     5701 cttcggcgac cggcggcgcc accacgatga gcgcggcccc ggcggccacc gtgtcgccga
     5761 gcttcacctt cacgtcggtg accgtgccgc cccacgtggc cgtgagttca ttctgcatct
     5821 tcatcgcctc gaggacggcc accggctggc cggccgccac cacctggccg gcctccacca
     5881 tcagcttgac caccgcgccg gggatcgggg cctggatcgt ctcaccgcgc gcggcgccgt
     5941 ccgacttccc ttccttcgcg cgggcccaga gctcgtcgaa cacctcgagc gaaaagagat
     6001 cgccgttcaa gtacacatcg cacttgtcgc cgtcctgcac ggtgttggcc tcgtacgact
     6061 tgccggcgat gatcagtgag cgcaccgcgg tgcgcccgac gcgccgcgag tcgacgacgt
     6121 gcgtcgtgcc gtcgatcgtc aattcgtagc gatcaccgac ttcgcggatg tcgaccgtga
     6181 cccgccggtt gccggcctga acgacgtagg tggccttcat cgccaccacc gcccggtgcg
     6241 gcccgccatc ttccaggcgc tgggcttctc accgtccccg ttcgttcccg tggtcgtccc
     6301 ggccgtgttg ccttcgtgca gatggatcac cgccgcgatc atggcggccg tctccgcttc
     6361 gggattcact tcgggtgtga gtcccttgaa gtcgcggtcg atcgtgcccg tgtcgaactc
     6421 ggccttctgg aattccggcc gctgcagcaa ccagcgatgg aacggaatcg tggtcttgag
     6481 cccgtcgatc tggtactcgg ccagcgcgcg cagcatgcgg cggcgcgcct cctcgcgatc
     6541 ctgtcccgac accgacagct tggccagcat cgggtcgtag tagatcggca cctcgtaccc
     6601 ctggtagaag ccgatgtcgt tccgcacgcc cggccccgac ggcatgcgca gccgtacgat
     6661 cgtgccggtg accggcagga agccgcgata cggatcctcg gcgtagatgc ggcattcgat
     6721 ggcccagccg cgcgggatca ggtcgatctg gttcagccgg agcgaaccgc cgccggcgat
     6781 gtggatctgc tccttgacca ggtcgacgtc gtacacgagc tcggtgaccg ggtgctccac
     6841 ctggagtcgc gtgttcatct cgaggaagta gaacttcccg tcgggcgcgt agatgaactc
     6901 gatcgtgccg gcgttcacgt agccggcggc gcgggcgatg gccagcgccg cctcgcccat
     6961 ctcctcgcgc tggtgcggcg tgatgacggg cgatggcgcc tcttcgacca ccttctggtg
     7021 acggcgctgg atggtgcact cgcgctcgcc caggtgcacg ccgttcccct tgccgtcgaa
     7081 cagcacctgc acctcgatgt ggcgcggccg ctcgatgtac ttttcgaggt acacctcgtc
     7141 gtcgccgaac gcgctggccg cctcgccttt cacgagcgaa agcgccgagc ggagctccgg
     7201 ctcgctccgc acgatccgca ttcccttgcc gccgccgccg gccgcggcct tcagcatcac
     7261 cgggtagccg atctcggtgg cgacgcgcac cgccgtgact tcgtcggcca ccggaccatc
     7321 gctgccggga acgaccggcg cgccggcctt ccgcgccgcc tcgcgggcca cgaccttgtt
     7381 tcccatcatc tcgatggcct cgggcggcgg cccgatgaac gtgacgccgg cctcgcggca
     7441 cttgcgggcg aagtcggcgt tctcggagag gaatccgtac cccgggtgga cggcgtcggc
     7501 gcccgaggct ttggccgcgg ccagcacgtt ctcggccacg aggtacgatt cgcgcgaggg
     7561 cgccggtccg accggccacg cctcgtcggc catcctcacg tggagcgcca gccggtcggc
     7621 ctccgagtag acggccaccg acgggatgcc gagctcgcgc aacgcgcgca tcacacggac
     7681 ggcgatttcg cctcggttgg ccaccaggac cttcttgaag gggcgcgaca tagggcggcg
     7741 atggtatcag gagggtggca cctcggctat cctgtccggc cgccgggaac gccggcgtcg
     7801 ctgcgaatct ttggaggtac ccgacttcat gcgcccagtc gttgcggttg ccccgctcat
     7861 cgtcatcggc ctggtcggcc tcacggccgg cacgggtctc gcccaggaat cggttccgca
     7921 gagttcagtg aatcggatcg cgcaccgttg gcaggtggac atc
//
LOCUS       DASVOD010000694         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_409569, whole genome shotgun sequence.
ACCESSION   DASVOD010000694 DASVOD010000000
VERSION     DASVOD010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409569"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..2102
                     /locus_tag="VF720_14925"
     CDS             288..2102
                     /locus_tag="VF720_14925"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX7880705.1"
                     /translation="MLDAMRKNTKVILWITVVSFLVLMVLVWGAEQQVGCGVSQGVIG
                     RVNGEPIHLEYFNRVYQANRDNFRQGRGTDPGPGDEAMLLEQTWSSVLEQVLLIQEAK
                     KRGLAPTDAELRHVAQQDPPAVFTSNPNFQTNGQFDHQKYTSLLASNSEFAHQLEAYL
                     RDTLPVEKLQSLVYQGAQVSDGDVRQAFLDRNETARITYRIWEMRNYPLPAAITDQEA
                     EAYFKAHPDEFNLPPQATVRAVKLDRKPTDEDIADLRRQLEDYAAIARRAASGDSTAQ
                     NFASLAETYSDLPNGAQGGLSDRFVGPGELGSELEAPLMSLPVGGVSDPIQDRQGMNL
                     VQVDSVMVENGVRKVRFRQLMLKLEPSDATVDALEEKMRGLKEKVTASRFAEMAQAAG
                     LTVQSPPAFAEGGFIRGLEAVPGPSTWAFKAPVGTISPVFTTNDAWYLLVLDRRSAKG
                     KPAFQDLVELARSKATEARQREMARKDAETFLALARTSGDWRRAAGADSNQVRIVGPF
                     SKAAGIPGLGRDPEPMAAVFSGPAGSIAGPLDATRGVMIVRVEERHGADESLLANQKA
                     QIETQLVSQRRNEVYSDWLKNLKDSAEIKDYRDLYFRS"
     gene            complement(2087..2617)
                     /locus_tag="VF720_14930"
     CDS             complement(2087..2617)
                     /locus_tag="VF720_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX7880706.1"
                     /translation="MKRLWAGWRMAYVEKTGPHDAGKCLFCRLGELEPSSSNLVLESG
                     EECLIVLNAFPYNCGHLMVAPRRHRPWTASLDASERAAVWDGIARAETALRKAYRPHG
                     INAGLNLGRPAGAGVVGHVHMHLVPRWNGDTNFMPVVAGTKVLPESLDVTWERLRTAL
                     AEGRSTARRAKGQLRK"
     gene            complement(2614..3369)
                     /locus_tag="VF720_14935"
     CDS             complement(2614..3369)
                     /locus_tag="VF720_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008183598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX7880707.1"
                     /translation="MMLLPEGSAAIILAAGKGTRMKSDLPKVAVPMAGRPLIRYVLDA
                     TRGAGITRVVAVVGYRRELVAEAMSGSQATLAVQEEQRGTGHAVMVAAPFLAGQKGPV
                     VVLAGDIPLIRAGTIASLLAQHEGTGAAVTVLTADLDDPHGYGRIIRLPDGRVTGIVE
                     ERDATPEQRSIREINSSIYAFDWAFLEPSLGRLTSHNAQGEYYLTDTLRMAFEQGLRV
                     EAMRVADPAEVSGVNTVEQLAELEAAWRVREGR"
     gene            complement(3366..>3650)
                     /locus_tag="VF720_14940"
     CDS             complement(3366..>3650)
                     /locus_tag="VF720_14940"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEX7880708.1"
                     /translation="LQAALRAGAALVRSGERRSATVLAAVRRRLAEAPLFRPQYIELV
                     DALTLAPVDRLSGRVILAAAGFLGKARLIDNIPLTVPGSRARRKAIGEIA"
BASE COUNT          613 a         1273 c         1184 g          580 t
ORIGIN      
        1 tgcttcttgt tcagccagcc ggtgccgtag atcgaggaga atggccccga acgggaggct
       61 ctgaaaaacg cgatccgtaa cttccggcgc cagggcgcgc attgtgggac tcataagtgg
      121 cgggagtcgt cctccgcgca caatgtgccg gaacgggcgg gatcgggtca ccgagaacgt
      181 gtgattgtgg aggcggtaac gcgggtgata tcatcaccgg cctcatttcc cgtcatgccc
      241 tgtgccggcg gtcaacgcac cgcctcgaac tttttcggga gccgtccatg ctcgacgcga
      301 tgcgcaagaa cacgaaggtc atcctctgga tcaccgtcgt ctcgttcctc gttctcatgg
      361 tcctggtgtg gggcgccgag cagcaggtcg gctgcggcgt cagccagggt gtcatcggtc
      421 gcgtcaacgg cgaaccgatc cacctcgagt acttcaaccg ggtataccag gccaaccggg
      481 acaatttccg gcaggggcgc ggcaccgatc cgggtcccgg cgatgaggcc atgctgctcg
      541 aacagacctg gtcgtcggtg ctcgagcagg tgctcctgat ccaggaggcc aagaagcgcg
      601 ggctcgcgcc gaccgatgcg gagctccggc acgtggccca gcaggatcca ccggccgtct
      661 tcaccagcaa tcccaacttc cagacgaacg gtcagttcga ccaccagaag tacacgtcgc
      721 tcctggccag caattcggag ttcgcccacc agctcgaggc ctacctgcgc gatacgctgc
      781 cggtggaaaa gctgcagtcg ctcgtgtacc agggggccca ggtctcggat ggggacgtgc
      841 gccaggcttt cctcgaccgg aacgagacgg cccgcatcac ctaccgcata tgggagatgc
      901 gcaactatcc ccttcccgcc gccattaccg accaggaggc ggaggcctac ttcaaggcgc
      961 accccgacga gttcaacctt ccgcctcagg ccaccgtccg ggccgtgaag ctggaccgga
     1021 aaccgaccga tgaggacatt gccgacctgc gccggcagct cgaggattac gccgccatcg
     1081 cgcggcgggc ggcctccggc gattccaccg cccagaactt cgcctcgctg gccgaaacct
     1141 actccgatct cccgaacggt gcgcagggcg gcctctcgga ccggttcgtc ggcccggggg
     1201 aactgggatc cgagctcgaa gcacccctga tgtcgctgcc cgtggggggc gtttccgacc
     1261 cgatccagga ccgccagggg atgaacctcg tccaggtcga ttcggtcatg gtcgagaacg
     1321 gcgtccgcaa ggtccgcttc cgccagctca tgctgaagct ggaaccgagc gacgccaccg
     1381 tcgatgccct cgaggagaag atgcgcgggc tcaaggaaaa ggtgacagcc tccaggttcg
     1441 ccgagatggc gcaggcggcc ggactcacgg tgcaatcccc ccctgctttc gccgagggtg
     1501 ggttcattcg cggactcgaa gcggtgccgg gcccttccac ctgggccttc aaggcgcctg
     1561 tcggcacgat cagccccgtc ttcaccacca acgatgcctg gtacctcctg gtgctcgacc
     1621 gccggagcgc caaggggaag cccgccttcc aggatctggt cgaactggcc cgcagcaagg
     1681 ccaccgaagc ccgccagcgt gaaatggcca ggaaggatgc cgaaacattc ctggcactgg
     1741 cccgcacctc gggcgactgg cggcgcgccg ccggtgcgga ctcgaaccag gtgcgcatcg
     1801 tcgggccgtt cagcaaggcg gcaggcattc cgggcctggg acgcgatccc gagccgatgg
     1861 cggcggtgtt cagcggcccg gccggaagca tcgccggtcc gctcgacgcc actcgcggcg
     1921 tcatgatcgt cagggtcgag gagcggcacg gcgcggacga atcgctgctt gccaaccaga
     1981 aggcccagat cgaaacgcag ctcgtctcgc agcgccgcaa cgaggtctac tcggactggc
     2041 tcaagaacct caaggattcg gcggagatca aggactaccg agatctctat ttccggagct
     2101 gacccttcgc gcggcgcgcg gtggaccgtc cctccgccag cgccgtgcgc aaccgttccc
     2161 aggtcacgtc caggctttca ggcaatactt tcgtgcccgc caccaccggc atgaaattgg
     2221 tgtcgccgtt ccagcgcggc accaggtgca tgtggacgtg ccccaccacc ccggcaccgg
     2281 ccggtcggcc gaggttgagc cccgcgttga tgccatgggg acggtacgcc tttcgaagcg
     2341 ccgtttcggc ccgggcgatg ccgtcccaca ccgcggcccg ctccgacgca tcgagcgagg
     2401 ccgtccacgg gcgatggcgc cggggcgcca ccatcaggtg gccgcagttg tagggaaagg
     2461 cgttcaggac gatcaggcac tcctcgcccg attcgagcac gaggttgctc gacgagggtt
     2521 caagctcccc caggcggcag aagagacact ttcccgcatc atgtggtccg gttttctcca
     2581 cgtaggccat ccgccatccc gcccagagcc gcttcatcgc ccctccctga cgcgccaggc
     2641 cgcttccagc tcggccagct gttccaccgt gttcacgccc gacacttcgg ccggatcggc
     2701 aacgcgcatc gcctcgacac ggagcccctg ttcgaaggcc atgcgcaacg tgtcggtgag
     2761 gtagtactcc ccctgcgcgt tgtggctcgt gagccggccg agcgagggtt cgaggaacgc
     2821 ccagtcgaag gcgtagatgc tcgagttgat ctcccggatc gagcgctgct ccggcgtggc
     2881 gtccctttcc tcgacgattc ccgtcacacg accatcgggg agccggatga tccggccgta
     2941 cccgtgcgga tcgtcgagat cggccgtgag cacggtcacc gcggcaccgg tcccctcgtg
     3001 ctgcgcgagg agcgaggcga tggtgccggc ccggatcagc gggatgtcgc ccgccaggac
     3061 caccacgggc cccttctggc cggcgaggaa gggcgccgcc accatgaccg cgtgacccgt
     3121 cccgcgctgt tcctcctgga ccgccaatgt ggcctgactc ccgctcatcg cctccgccac
     3181 gagctcgcgc cggtatccca ccaccgccac gacgcgcgtg atgccggcgc cccgggtggc
     3241 atcgagcacg taccggatga gcggccggcc ggccatcggc accgcaacct tggggaggtc
     3301 ggatttcatc cgtgttccct tgccggccgc caggatgatg gccgccgacc cttccggaag
     3361 aagcatcatg caatttctcc gatcgctttc cgccgggctc ttgagccggg cacggtcagc
     3421 gggatgttgt cgatgagccg ggcctttccg aggaagcccg cggccgccag gattacgcgc
     3481 ccggacaggc gatcgaccgg tgccagcgtg agggcatcga ccagctcgat gtactggggt
     3541 ctgaagagcg gcgcctcggc aaggcgcctg cgaacggcgg ccagcaccgt ggcgcttcga
     3601 cgctcgccgc tccggacgag cgccgctccg gccctgagcg cggcctggag
//
LOCUS       DASVOD010000695         3606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_986494, whole genome shotgun sequence.
ACCESSION   DASVOD010000695 DASVOD010000000
VERSION     DASVOD010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986494"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VF720_14945"
     CDS             <1..570
                     /locus_tag="VF720_14945"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX7880709.1"
                     /translation="HPQPESYWGNVNPIGVRGMYDEAKRFAEALTMAYHRYKGVDTRI
                     VRIFNTFGPRMRKDDGRAVPTFINQALLGEPLTVFGDGSQTRSFGYITDLVEGLYALM
                     QSSEHEPVNIGNPQEMTVKEMAETIIAITKSKSSIVYKPLPEDDPKVRQPDITKAKRV
                     LGWEPKVPVKDGLIRTIEWFVANHGPARA"
     gene            567..1289
                     /locus_tag="VF720_14950"
     CDS             567..1289
                     /locus_tag="VF720_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HEX7880710.1"
                     /translation="MNKALVIVPTYNEADNVRELIPAILDQGPNFEVLIVDDNSTDGT
                     GDIIEEMRKGEPRLHCLHRPGKMGLGTAYRDGFKWALARDYEFIYEMDADFSHDPGSL
                     PAFIKAAEETGADLVLGSRYLNGVTVVNWPLSRLILSYGANVYTRVITWMPVNDATGG
                     FKCFRRKVLAALDLDRVKSDGYGFQIEMSFKAWRKGFKLKEIPITFTDRRVGVSKMNG
                     RIIREAMWLVWWLRLSALLGRM"
     gene            1760..2809
                     /locus_tag="VF720_14955"
     CDS             1760..2809
                     /locus_tag="VF720_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX7880711.1"
                     /translation="MTFLFIGGTQFVGRHLVEAARAAGHDVTLFHRGKTNPGLFPDIE
                     HIHGDRATDIGKLAGRRWDRVIDLCGYLPRVVTLSADALRLNAAHYTFISSISVYKEL
                     DTVTVASGMPPDPIDEMRSVIELADPTVEEITGETYGGLKYLCEQAARQVFGHTRVLI
                     VRPGLIVGPYDHTDRFTYWCHRAAAGGVMVVPGSPERHFPVIDVRDLVAWILPMSDRA
                     ASGVVNLGGPNVDGFRGGYDTGPEPTFANLLEAVGRATGASIEPVWMSSETMSRHGID
                     PESFPLWNQDAAGHEATFAIDSDRAFGSGLRCRPLADTVRDTLAWRTPLLSELPLKVG
                     PTREREAEMMRLVRG"
     gene            2876..>3606
                     /locus_tag="VF720_14960"
     CDS             2876..>3606
                     /locus_tag="VF720_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880712.1"
                     /translation="MIDAPGTVRSRRLRWFPLAVVFASLLPATRPAFAVCPPLFDGYI
                     SSFGTAPGQIRYPNGLATDAAGRIYVTDPAGEFVHVFEADGTFVTRWGGFGGGLGQFN
                     ELVNLTVHGNYVYAVELDGCRVQFFTLSGGIVGAIGGSGSTDGRFLAPRGVGIGPTGT
                     IYVADTGNDRIQYFTSQGAYLGQWAIEDPFDVDTDDAGDVYVVSHVGNIHRFDSVGNP
                     VAVWSGSIYGGLHQPFGLDVANGYV"
BASE COUNT          643 a         1180 c         1136 g          647 t
ORIGIN      
        1 catccgcagc ccgagtcgta ctggggcaac gtcaatccga tcggcgtgcg cggcatgtac
       61 gacgaggcca agcgcttcgc cgaagcgctc acgatggcct accaccgcta caagggggtg
      121 gacacgcgga tcgtgcgcat cttcaacacg ttcggcccgc gcatgcgcaa ggacgacggc
      181 cgcgcggtgc ccacgttcat caaccaggcg ctcctcggcg agccgctcac cgtcttcggc
      241 gacggcagcc agacccgctc gttcggctac atcaccgacc tggtcgaggg gctctacgcg
      301 ctcatgcagt cgagcgaaca cgagccggtc aacatcggca atccgcagga gatgacggtg
      361 aaggaaatgg ccgagacgat catcgccatc acgaagtcaa agagctcgat cgtctacaag
      421 ccgcttccgg aagacgaccc caaggtgcgc cagcccgaca tcaccaaggc caagcgcgtt
      481 ctcggctggg aaccgaaggt gcccgtgaaa gacggcctca tccgcacgat cgagtggttc
      541 gtggccaacc atgggcccgc acgcgcgtga acaaagcgct cgtcatcgtt cccacgtaca
      601 acgaggccga caacgtccgc gagctgatcc cggccatcct cgaccagggc ccgaacttcg
      661 aagtgctcat cgtggacgac aactccacgg acggcaccgg cgacatcatc gaggaaatgc
      721 ggaagggaga gccgcgtctc cattgcctcc accgccccgg caagatgggc ctcggcaccg
      781 cgtatcgcga cggcttcaag tgggcgctgg cgcgcgatta cgaattcatc tacgagatgg
      841 acgccgactt cagccacgac cccggcagcc tcccggcctt catcaaggcc gccgaggaaa
      901 ccggcgccga cctcgtgctc ggcagccgtt acctgaacgg cgtcaccgtc gtgaactggc
      961 ccctctcccg cctcatcctg tcgtacggcg cgaacgtcta cacgcgcgtc atcacctgga
     1021 tgcccgtcaa cgacgccacc ggcggcttca agtgtttccg ccggaaagtc cttgccgcgc
     1081 tcgacctcga ccgcgtgaag tccgacggct acggcttcca gatcgagatg tcgttcaagg
     1141 cctggcgcaa ggggttcaag ctcaaggaaa tccccatcac cttcaccgac cggcgcgtgg
     1201 gcgtctcgaa gatgaacggg cggatcatcc gcgaggcgat gtggctggtg tggtggttgc
     1261 gattgtcggc gttgctgggg cggatgtagg gactgcgacg ttcacgtact ctcctcaaga
     1321 gagtaaagca agcttcggcg tagaaattcg ggatatctga ggcagccgcc cgctctcggc
     1381 gttgcgcacc tccctgcgat actgtcaggg atcgagatcg aaatctcgat ccgcacgctc
     1441 ctcggccaac gcctcgagaa tgtccccggt atgctcgaaa gggtggaccg tcagggatgt
     1501 cgcccatggt cttgtatttc accttgggcg gatggatggt caacccaacc ctcagcgatg
     1561 aataggggat gatgctggca accagttggt ctttctccag gataacgaca gtttcaccct
     1621 tccgaacttt cttcaggtat ttccggagac tggtctccag atccgcgacg ccgatgatga
     1681 tcatgcgcga ttgtgcccac gcctcgtttc gagctgtcaa tccattttcc cgccgaaaga
     1741 atcctgctac ggtctcccca tgaccttcct cttcatcggc ggcacccagt tcgtcggacg
     1801 acacctggtg gaagcggccc gcgcggcggg ccacgacgtc acgctctttc atcgcggcaa
     1861 gaccaatccc ggcctctttc ccgacatcga gcacattcat ggtgaccggg ccaccgatat
     1921 cggcaagctg gcggggcggc ggtgggaccg tgtcatcgac ctgtgcggct acctgccgcg
     1981 cgtcgtcacg ctgtcggcgg atgcgctgcg gttgaacgcc gcccactaca cgttcatctc
     2041 gagcatctcg gtgtacaagg agctcgacac ggtgaccgtg gcaagcggaa tgccgcccga
     2101 tccgatcgac gagatgcgat cggtcatcga gttggccgac cccacggtcg aggagatcac
     2161 cggcgagacg tacgggggcc tcaagtatct ctgcgagcag gcggcgcggc aggtattcgg
     2221 gcacacgcgg gtgctgatcg tgcgccccgg gctcatcgtc gggccgtacg accacacgga
     2281 tcgattcacg tactggtgtc atcgcgccgc cgcaggcggt gtgatggtgg tgcccggctc
     2341 cccggaacgg catttccccg tgatcgatgt ccgcgacctg gtggcctgga tcctgcccat
     2401 gtctgatcgc gcggcaagcg gcgtggtgaa cctgggcggt cccaacgtgg atggttttcg
     2461 cggcggttac gacacggggc ccgagccgac attcgccaac ctgctcgagg cggttggccg
     2521 cgccaccggc gcgtccattg aaccggtctg gatgtcgtcc gaaacgatgt cacgccacgg
     2581 gatcgatccg gaatcgttcc cgctctggaa ccaggacgcg gccgggcacg aggccacgtt
     2641 cgccatcgat tcggaccgcg cgttcggttc ggggctgcgt tgccggccgc tggccgatac
     2701 cgtgcgcgac acgctggcgt ggcggacgcc gctgctgtcg gaactgcctc tcaaggtggg
     2761 gcccacgcgg gagcgtgagg cggagatgat gcgactcgtt cgggggtgaa aggcgtgtga
     2821 tacgctggcg tcacgcaagc cgatgacgcc acgcctgacg tggaggccgc tcctgatgat
     2881 cgacgcaccc ggcaccgtga gatcccgtcg cctgcgctgg tttcctcttg cggtggtgtt
     2941 tgcctcactc ctcccggcaa cccggcccgc gtttgccgta tgcccgcccc tcttcgacgg
     3001 atacatcagc tccttcggaa ccgctcccgg gcagattcgc taccccaacg gtctggccac
     3061 ggacgccgcg gggaggatct acgtgacgga tcccgccggg gagttcgtgc acgtgttcga
     3121 ggccgacggg accttcgtca cccgttgggg aggcttcggc ggtggcctcg gtcagttcaa
     3181 tgaactggtg aacctcacgg tccatggcaa ctatgtctac gccgtggagc tggacggctg
     3241 ccgcgtccag ttcttcacat tgagcggagg aatcgtcggc gccatcggcg gcagtggttc
     3301 gaccgacggt cgattcctcg caccacgcgg cgtggggatc ggtccgacgg gaaccatcta
     3361 cgtggccgat acggggaacg accggatcca gtacttcacc agccagggcg cctatctcgg
     3421 acagtgggcc atcgaggacc cgttcgacgt cgacacggac gacgccggcg atgtctatgt
     3481 cgtgtcgcac gtggggaaca tccatcgttt cgactccgtc ggaaacccgg tggcggtatg
     3541 gagcggctcg atctacggcg gactgcacca gcctttcggc ctcgacgtgg ccaacggtta
     3601 cgtcta
//
LOCUS       DASVOD010000696         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_775287, whole genome shotgun sequence.
ACCESSION   DASVOD010000696 DASVOD010000000
VERSION     DASVOD010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775287"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1036
                     /locus_tag="VF720_14965"
     CDS             <1..1036
                     /locus_tag="VF720_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880713.1"
                     /translation="EARLDLGDARIARRLIPWVRGFAGLEAMTRGSVSFTWRVGAAPP
                     IAPADATPGAIADYYHLTGTIEEGRVVYRTTDGGWMALAADGDRVDFLVPESMLTGPD
                     WSLRDLFSAAITSLLRRQGRFPIHAAGVARGAAALATGEAGVGSPPAGVLIVGAPMAG
                     KTSLALNFIRRGWTWLSDDKIALEGAAEGVRMSGLYRPANVDPALARWFPEMSGIESR
                     APAFPNSEKRSVAIDAIYGHSAFSGADSAPARVTHLLFPRVVDIGPTRLTPLTPGEAL
                     VRMMTQSPIHADRDDARRQLALLSRLAGSAAAFAIDAGRDLLDSPDTLVTAGRTMGLA
                     IDLPVTSPPV"
     gene            1047..1961
                     /locus_tag="VF720_14970"
     CDS             1047..1961
                     /locus_tag="VF720_14970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7880714.1"
                     /translation="MTTPFVSVVVPVLNGGKHLGELLRSLAALDWPADRLEILVVDNG
                     STDDTAEQARAAGVTLLSEPERGASRARNRGIREARGEIVAFTDADCLVTRRWIRGVT
                     APFQDPVVGGVGGRIETYRPTTLAERYAARIGHLDVERHLSHPTFPFAPTANAAFRRD
                     VFEKIGGFDPDFPWGEPVDFGKRVVRDAGFRLAFAPTAVVLHRARASVAEFRRQQEGY
                     GYSLALLCSKYRDEVVWDVRRDREADAEVSRAFWRMPSAFVTRPEDGDRRATIQNRWL
                     DWVRAAARRRGFRRAVRERGLVFNRGAA"
     gene            1976..>2190
                     /locus_tag="VF720_14975"
     CDS             1976..>2190
                     /locus_tag="VF720_14975"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7880715.1"
                     /translation="MVSIIVPVRNGGRQLAQLVRSLERVDYPGPAPEIVIVDNGSTDG
                     AVAALAGRGTPEVRLRILSEPRPGAPCA"
BASE COUNT          299 a          769 c          771 g          351 t
ORIGIN      
        1 cgaagcccgc ctcgatctcg gcgacgcgcg gatcgcccgg cgactgatcc cgtgggtgag
       61 ggggttcgcg ggtctcgaag caatgacgcg cgggagcgtg tccttcacgt ggcgggtcgg
      121 cgcggcgcct ccgatcgctc ccgccgacgc cacccccgga gcgattgccg actactatca
      181 cctgaccgga accatcgaag aggggcgcgt cgtctaccgc acgaccgacg gcggctggat
      241 ggcgcttgcg gcggatggcg accgcgtcga cttcctggtt cccgaatcga tgctgaccgg
      301 cccggactgg tcgttgcgcg acttgttcag cgcggccatc acctcgctcc tccgccggca
      361 gggacgattc ccgatccacg cggcgggcgt ggcgaggggt gcggccgccc tggcgacggg
      421 tgaggccggg gtcgggtcac caccagcggg cgtgctcatc gtcggggcgc cgatggcggg
      481 caagacatcg ctggcgctca atttcatccg gcgcggctgg acctggctct cggatgacaa
      541 gatcgcgctg gagggggcgg ccgagggcgt ccggatgagc ggcctctatc ggccggccaa
      601 cgtcgatccg gcgctcgccc ggtggtttcc ggaaatgtcg gggatcgagt cccgcgcgcc
      661 cgcctttccg aacagcgaga agcgctcggt ggcgatcgac gcgatctacg gccattcggc
      721 attcagcggc gccgattcgg cgcccgcccg cgtcacgcac cttctcttcc cgcgcgtcgt
      781 cgatatcggc ccgacacgac tcacgcccct cacgccgggc gaagcgctgg tccgcatgat
      841 gacgcagtca ccgatccatg cggatcgtga cgacgcgcgg cgccaactcg ccctcctctc
      901 ccgcctggcc gggtcggccg ccgcgttcgc gatcgatgcc ggccgcgacc tgctcgactc
      961 tcccgacacg ctcgtgaccg ccgggcgcac gatgggactc gccatcgacc tgccggtgac
     1021 gtctcctccg gtgtgacggc ctgtccgtga cgacaccctt cgtttcggtc gtcgtgccgg
     1081 tcctcaacgg cggcaagcac ctgggtgagc tgctccgttc gctggccgcg ctcgactggc
     1141 cggccgatcg cctcgagatc ctggtagtcg acaatggatc gaccgacgac accgccgaac
     1201 aggcgcgggc ggccggcgtg acgctcctct ccgaaccgga acgcggcgcc agccgcgccc
     1261 gcaaccgcgg catccgcgag gcacgcgggg agatcgtggc attcaccgac gccgactgcc
     1321 tggtgacgcg tcgctggatc cggggagtca cagccccctt tcaggatccg gtcgtcggtg
     1381 gcgttggtgg gcgcatcgaa acgtatcgac cgaccacact ggccgagcgc tacgcggccc
     1441 gcatcgggca tctcgacgtg gagcgccatc tctcgcatcc cacgtttccc tttgccccga
     1501 ccgccaacgc ggctttccgt cgcgacgtgt tcgaaaaaat cggcggcttc gacccggatt
     1561 ttccgtgggg cgagccggtc gacttcggaa agcgcgtggt gcgcgatgcc ggcttccgac
     1621 tcgctttcgc gccgacggcg gtggtcctgc atcgggcacg cgccagcgtg gctgaattcc
     1681 gccggcagca ggaaggctac ggctacagcc tcgccctcct ctgcagcaag tatcgggacg
     1741 aggtggtgtg ggatgtccgc cgtgaccgtg aggccgatgc cgaggtgtcg cgggcgttct
     1801 ggcggatgcc gtccgcattc gtgacgcgcc ccgaggatgg tgaccgtcgc gccacgatcc
     1861 agaatcgctg gctcgactgg gttcgggcgg cggcgcgccg gcgcggcttc cggcgcgcgg
     1921 tgagggagcg gggcctcgtc ttcaaccggg gggccgcatg acggcggagt ggcccatggt
     1981 gtcgatcatc gttccggtcc ggaacggcgg acggcaactc gcgcaactgg tgcgatcgct
     2041 cgagcgagtc gactatcccg ggccggcgcc ggagatcgtg atcgtcgaca atgggtcgac
     2101 cgacggcgcg gtcgcggcgc tggcgggacg cggcacgccg gaggttcggc tccgcatcct
     2161 ctccgaacca aggcccggtg caccctgtgc
//
LOCUS       DASVOD010000697         2254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_99268, whole genome shotgun sequence.
ACCESSION   DASVOD010000697 DASVOD010000000
VERSION     DASVOD010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99268"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VF720_14980"
     CDS             <1..553
                     /locus_tag="VF720_14980"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEX7880716.1"
                     /translation="QVDGWGGKGSGLLMLPIIALVCYVIMALVPRIDPARANFASFAG
                     PFNLFRLVFLLFMAGLYAAMHATFRGAQLNMTSVVLPMLGLLFMVVGWMMGHIRPNWT
                     VGIRTPWTLSSRLAWDRSHRLGSKLFMAAGPVFVLAGIVGKPWAFGAAMCVMLAVVLV
                     TVGYSYVVWKNDPDREPPIVGRR"
     gene            complement(602..1582)
                     /locus_tag="VF720_14985"
     CDS             complement(602..1582)
                     /locus_tag="VF720_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012886946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEX7880717.1"
                     /translation="MKAIVIDTPGEEGVMRLGDAPEPVAGPEEVLIDVEATSVNRADL
                     YQRQGKYPPPTGASAILGLDAAGTVREVGARVRRFRAGDHVMALLPGGGYAERVVAHH
                     GSVMRVPAGWSMEEAAAFPEVYLTAFLNIFRVGAAREGETLLMHGGLSGVGTAAVQLA
                     REAGLRVIVTVGSPDRVTRALDLGADAAFDYHDDWAAAVAALGPVDVILDPIGARYLE
                     AHLALLATGGRLVAIGGMGGVRRAEIDWPTLMSKRISLTGSTLRARSVEEKGAIVLDL
                     EDRFGAAIAEGHLKPVVDQVFPLAEAPEAHRRMAASEHVGKIVLRVSGTD"
     gene            complement(1579..>2254)
                     /locus_tag="VF720_14990"
     CDS             complement(1579..>2254)
                     /locus_tag="VF720_14990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7880718.1"
                     /translation="APRVVRAHVSFHLPPGQPGMPAWPGADVTRSEATHLLDLRPGFD
                     HIWSRLFDGDIRRANARFEREGLTVVTGRDADAVRDLDHLYREQAADWTNHTRFPEAF
                     LPAVIAGLPDNALIFRAMSGHVAVAALLVLFDEQNAWTYLAPTSPEGRERLAPTVLYR
                     HAIEAMAARGLSWYHFGGSRGHHPLEKFKESLGGRPHADAECLVEAAWFRPLHHLQYR
                     LRGIRE"
BASE COUNT          323 a          789 c          798 g          344 t
ORIGIN      
        1 ccaggtggac ggctggggag ggaaggggag cggactcctg atgctcccca tcatcgccct
       61 cgtctgctac gtcatcatgg ctctcgtccc gcggatcgac ccggcgcgcg ccaacttcgc
      121 gagcttcgcc gggccgttca acctgtttcg ccttgtcttc ctgctcttca tggccgggct
      181 ctacgccgcc atgcacgcca cgttccgtgg cgcgcaactc aacatgacat cggtcgtcct
      241 gccgatgctc gggctcctct tcatggtcgt cggctggatg atgggccaca tccggcccaa
      301 ctggaccgtc ggcatccgca ccccgtggac gctctcgagc cgcctggcct gggatcgctc
      361 gcatcgcctc ggttcgaagc tcttcatggc ggccggacct gttttcgtgc tggccggaat
      421 cgtggggaag ccatgggcgt tcggcgcggc catgtgcgtg atgctggcgg tcgtgctggt
      481 gacggtcggt tactcgtacg tggtctggaa gaacgatccg gatcgcgagc caccgatcgt
      541 gggacggcgg tagggggggc gacagcggca tcgcgggcgg ggcgaccggt gtggtcggcc
      601 gtcagtccgt tcccgacacc cgcaacacga tcttcccgac gtgctcgctc gcggccatgc
      661 gacggtgcgc ctccggcgcc tcggccagcg ggaagacctg atcgacgacc ggtttcaggt
      721 gcccctcggc gatggcggcc ccgaatcgat cctcgaggtc gagcacgatg gccccctttt
      781 cctcaaccga ccgagcgcgc agagtcgatc cggtgagcga aatccgtttc gacatcaggg
      841 tcggccagtc gatctcggcc cggcggacgc cgcccatccc gccgatcgcc acgagtcgcc
      901 cgccggtggc cagcagggcg aggtgcgcct ccaggtagcg cgccccgatc gggtcgagga
      961 tgacatcgac ggggcccagc gcggccacgg ccgccgccca gtcatcgtgg tagtcgaagg
     1021 cggcgtcggc gccgaggtcg agggcacgcg ttacccggtc gggcgagccg acggtcacga
     1081 tgacgcggag ccccgcttcg cgggccaact ggacggcggc ggtgccgacg cccgacaggc
     1141 cgccgtgcat gaggagcgtc tcgccctccc gggcggcccc cacgcggaag atgttgagga
     1201 aggcggtgag atacacctcc ggaaacgccg cggcctcttc catcgaccag cctgcgggga
     1261 cgcgcatcac cgatccgtga tgcgccacca cgcgttcggc gtagccgccg ccgggcagca
     1321 gcgccatgac gtgatcgccc gcgcggaacc ggcgcacgcg ggcgccgacc tcccgcacgg
     1381 tgccggcggc gtccaggccg aggatggccg aagcgccggt cggcggcgga tacttcccct
     1441 gtcgttgata caggtcggcg cggttgaccg acgtggcctc gacgtcgatc agcacctcct
     1501 ccgggccggc cacgggctcc ggcgcgtcgc cgaggcgcat cacgccctcc tcgcccggcg
     1561 tgtcgatgac gatggccttc attcgcgtat cccgcgcagt cgatactgaa ggtgatgcag
     1621 ggggcggaac caggccgcct cgacgagaca ctcggcgtcg gcgtgcggcc gtccacccag
     1681 gctttccttg aacttctcga gggggtgatg cccgcggctg ccgccgaagt gataccaact
     1741 caggccgcgc gcggccatgg cctcgatggc gtgccgatag aggacggtcg gcgccaggcg
     1801 ctcgcgcccc tcgggggaag tgggcgccag gtaggtccag gcattctgtt catcgaacag
     1861 gacgagcagc gccgccacgg cgacgtgtcc cgacatcgcc cggaagatga gcgcgttgtc
     1921 cggcaggccg gcgatcaccg cgggaaggaa ggcctccgga aaacgggtgt ggttcgtcca
     1981 gtcggccgcc tgttcgcgat acagatgatc gaggtcgcgc acggcatcgg cgtcgcgccc
     2041 ggtcacgacc gtgagtcctt ctcgctcgaa ccgcgcgttc gcgcgccgga tgtcgccgtc
     2101 gaacagccgc gaccagatgt ggtcgaaccc cgggcgcagg tcgaggaggt gcgtggcctc
     2161 ggaccgcgtg acgtccgcgc cgggccaggc cggcatcccc ggctggccgg gcggcaggtg
     2221 gaacgacacg tgcgcgcgca ccacccgcgg ggcg
//
LOCUS       DASVOD010000698         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_357661, whole genome shotgun sequence.
ACCESSION   DASVOD010000698 DASVOD010000000
VERSION     DASVOD010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357661"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /gene="tssF"
                     /locus_tag="VF720_14995"
     CDS             <1..1040
                     /gene="tssF"
                     /locus_tag="VF720_14995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX7880719.1"
                     /translation="FTFPQKFLFVEISGLARLAELGGEDQFDIVLRLDRPPTNALRLS
                     PDNFLLGCAPAINLFEKDGDPITVEHDKTEYLVRASGRDPGHYEIFSVDRVRGIVPGT
                     AEPREYPSFLSYIQSPGSGAAGSVYHGVRLKPAVVADGTDSWLSFHAAPGDPALPDTE
                     TISVSLTCTNRRLARSLRIGDVSVATTTSPGFAQFRNIGSVTTSLPPPLGGDLHWRLI
                     SHLALSQQGMSTVEALRTVLSLYNFQAMHDRQAARENELRLAGIRSLRAEPADAVIGG
                     AVARGRHTILELDETNFSGPGDLVLFASVLDEFLAQYASLNHVSRLTVKTTGSGEEYA
                     WKPRFGRRTTL"
     gene            1004..>1653
                     /gene="tssG"
                     /locus_tag="VF720_15000"
     CDS             1004..>1653
                     /gene="tssG"
                     /locus_tag="VF720_15000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HEX7880720.1"
                     /translation="MEAPLRSPHHPLISRLEREGHRFNLYQAIYLLERASPNAVRLGG
                     TGPAGGEALRLRPDVSLGFPAGSIEKIEALDERRWRIEATFFGLYGADSPLPTHWAED
                     VLHEQSFDTTVRDFLDIFHHRIYSLLYRTWAKYRFTVQYTGNTEDSLTDRLLCLIGLG
                     SPEVRAATGLPVTRLLRWAGLLVQQPRSAAGLEVLLSDWFGGVPVRLTPAVGRWVPL"
BASE COUNT          239 a          597 c          536 g          281 t
ORIGIN      
        1 atttcacttt cccgcagaag ttcctgttcg tcgagatctc cggcctcgca cgcctcgcgg
       61 agcttggcgg cgaggaccag ttcgacatcg tgctgcgcct cgaccgcccg ccgaccaacg
      121 cgctccgcct ttcgcccgac aatttcctgc tcggctgcgc acccgccatc aacctgttcg
      181 aaaaggatgg cgatccgatc accgtggagc acgacaagac cgagtatctc gtccgcgcca
      241 gcgggcgtga tccggggcac tacgagatct tctcggtcga tcgcgtgcgg gggatcgtgc
      301 cggggacggc ggagccgcgc gagtatccgt cgttcctctc ttacatccag tcacccggca
      361 gcggggctgc cgggagtgtt taccacggcg tgcggctgaa gccggccgtg gtcgccgatg
      421 gcaccgattc gtggctgtcg ttccacgccg cgcctggtga cccggcgctg ccggacaccg
      481 agacaatctc cgtgagcctg acctgcacga accgccgcct cgcccggagc ctgcgcatcg
      541 gcgacgtgtc ggtggccacc acgacgtcac cgggcttcgc ccaattccgc aatatcggca
      601 gcgtgaccac gtcactgccg ccgccgctcg gcggcgatct ccactggcgg ctcatctcgc
      661 acctggcgct ctcgcagcag gggatgtcga cggtggaggc gctgcgcacg gtcctctcgc
      721 tctacaactt ccaggcgatg cacgatcgcc aggccgcccg cgagaacgag ctccggctgg
      781 ccggcatccg atcgttgcgc gccgaaccgg ccgacgccgt gatcggcggc gcggtggccc
      841 gcggacgcca caccatcctc gaactcgacg aaaccaactt ctcgggcccg ggcgacctcg
      901 tgctctttgc ctcggtgctg gacgaattcc tcgcccagta cgccagcctg aaccatgtca
      961 gccgcctgac ggtgaagacg accggctccg gcgaggagta tgcgtggaag ccccgcttcg
     1021 gtcgccgcac caccctctga tcagtcgact ggagcgggaa ggacaccgct tcaacctgta
     1081 ccaggccatc tacctcctcg agcgcgcctc gccgaacgcg gtgcgtctcg gcgggacggg
     1141 gccggcgggc ggtgaggcgc ttcggctgcg gccggacgtg tcgctcggct ttccggccgg
     1201 gtcgatcgaa aagatcgaag cgcttgacga acggcggtgg cggatcgagg ccaccttctt
     1261 cggcctgtac ggcgccgact cgccgctgcc cacgcactgg gccgaggatg tgctgcacga
     1321 gcagtcattc gacacgacgg tgcgcgactt cctcgacatc ttccatcacc ggatctactc
     1381 gcttctgtac cgcacgtggg ccaagtaccg cttcacggtg cagtacaccg gcaatacgga
     1441 agactcgctc accgaccggc tcctctgcct catcggcctg ggatcgcccg aggtgcgggc
     1501 ggccacgggg cttccggtga cgcggctgct tcgctgggcc ggccttctcg tgcagcagcc
     1561 gcgctcggcg gccgggctcg aggtgctcct gtccgactgg ttcggcggcg tgccggtgcg
     1621 cctgacgccg gccgtcggcc gctgggtgcc gct
//
LOCUS       DASVOD010000699         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_618901, whole genome shotgun sequence.
ACCESSION   DASVOD010000699 DASVOD010000000
VERSION     DASVOD010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618901"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1705)
                     /locus_tag="VF720_15005"
     CDS             complement(<1..>1705)
                     /locus_tag="VF720_15005"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEX7880721.1"
                     /translation="EANLLLTGRLRLFGTLHDVRRAQDEGDALRLDWNFHPAETPFEP
                     FRKDPKFPWEVARLAALPRLGLAYFISGRGRYARAAASIVEDWSVTQPVGQGLHFASA
                     LEVGIRLIAMMQAFHFFRRSEAFQAQTLATLVRRIAREAAWLQGHRSVERLVAGNHLV
                     GELAGLVVTDLTFPELGQGNRLEDNLARFARAIDEQVAPDGTSLEQSATYGRFVADFV
                     AAVLATAAAAGHPVPAGLVDRAAALAGWLGALTTPDGQLPLMGDNDGGRGVDWAETVP
                     SSDARGVIMALAALTGRPEPLLALGRPGAPVATHGYGPDGAELTWWYAGPDGLARLTA
                     LRQNMPPPRALRHFPDGGWAVATGSAPDHLVVRCGPFGHGLPKPSGHSHADWMAPVLT
                     LDGTPLLVDPGNFGYTTVGEARQAFRSEEAHAVLSFEGAPMAVPGTTFRWDDIPAPAR
                     LVAAQPDSDSIELAGSWRTTGGSRSVAARRVIVYNCSRSRVDIRDDWSAGGDPPAAVV
                     QRWPLAAGSEVTAGAGGTGFEVRTDGGAVFLFSIEPAASIEVERRPLAPQYAAQRPAI
                     RV"
BASE COUNT          258 a          596 c          618 g          233 t
ORIGIN      
        1 cgacccggat ggctggccgt tgggccgcgt actgcggcgc cagcggccgg cgttccacct
       61 cgatcgaggc ggccggttcg atcgaaaaca gaaagaccgc accaccgtcg gtccggacct
      121 cgaatccggt ccctccggcg cccgcggtca cctcacttcc ggccgccagg ggccatcgct
      181 gcacgaccgc cgcgggggga tcgccccctg cgctccaatc gtcacgaatg tccacccggg
      241 accgcgaaca attgtagacg atgacccggc gggcggccac cgaccggctt ccccccgtcg
      301 tgcgccagct gccggcgagt tcgatcgagt cgctatcggg ctgcgctgcg acgaggcgtg
      361 ccggcgcggg gatgtcgtcc cagcggaacg tggtgccggg cacggccatc ggcgccccct
      421 cgaaggacag gaccgcatgg gcttcttccg agcggaacgc ctggcgggcc tcgcccaccg
      481 tggtgtagcc gaaattgccg ggatcgacga ggagcggcgt gccgtccagc gtgagcaccg
      541 gggccatcca gtcggcatgg ctgtgaccgg agggcttggg aagcccgtgc ccgaacgggc
      601 cgcaccggac gaccaggtgg tccggggcac tgccggtggc cacggcccaa cccccatcgg
      661 gaaagtggcg cagggcgcgg ggcggcggca tgttctggcg aagcgccgtc aatcgggcca
      721 ggccatccgg accggcgtac caccaggtga gctcggcgcc gtcgggtcca taaccatgcg
      781 tggcgaccgg ggcgccgggg cgcccgagcg cgagcagcgg ttccggacgc ccggtcagcg
      841 cggccagggc catgatgacg ccccgggcat cgctcgaggg aaccgtttcg gcccagtcga
      901 caccgcggcc gccgtcgttg tcccccatga ggggaagctg cccgtcgggc gtggtgagtg
      961 ctcccagcca tcccgcgagg gccgccgccc gatccacgag gccggccggg acgggatgcc
     1021 ccgcggcggc cgccgttgcc aggaccgcgg ccacgaagtc ggccacgaag cggccgtacg
     1081 tggccgattg ctcgaggctc gtgccgtccg gggccacctg ctcgtcgatg gcccgggcga
     1141 atcgggccag gttgtcctcg agccgatttc cctgtcccag ttcgggaaac gtgaggtccg
     1201 tgacgacgag gccggccagt tcaccgacca ggtgattgcc ggccaccagt cgctccaccg
     1261 atcgatgccc ctgcagccag gccgcttccc gggcgattcg tcgcacgagc gtggcgagcg
     1321 tctgcgcctg gaaggcttcg gatcgccgga agaagtggaa cgcctgcatc atcgcgatga
     1381 ggcggatgcc gacttcgagg gcgctcgcga aatggagtcc ctggcccacc ggttgcgtga
     1441 cgctccagtc ctcgacgatc gaagcggccg cccgtgcata gcgcccgcgc ccggaaatga
     1501 aatatgcgag gccgagccgc gggagcgcgg ccagtcgcgc cacttcccag gggaacttcg
     1561 gatccttccg aaagggttcg aagggggttt ccgcgggatg gaagttccag tccaggcgaa
     1621 gcgcgtcccc ttcgtcctgg gcccggcgga cgtcatgaag cgtgccgaag agtcggaggc
     1681 gcccggtcag gaggaggttg gcctc
//
LOCUS       DASVOD010000700         7880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_49362, whole genome shotgun sequence.
ACCESSION   DASVOD010000700 DASVOD010000000
VERSION     DASVOD010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7880
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49362"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VF720_15010"
     CDS             <1..688
                     /locus_tag="VF720_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880722.1"
                     /translation="MRRAEGNDAEIALALEGVGWAQLLGGNDRAALDTFREFLGAQLA
                     AGDPVMVNRARVAVAQVEVALGHLDEARALSKEIMAFSRGRGDRRNEHFAAHFLADCD
                     LIEENYAAALHRYRDSLALVWAIGDRLESSFEVQGSAMSLAGLGRADEALRLAGAVEA
                     EWERLGIEPHMGFWDRLLDRYLVSARSAGGAAAEAAWQYGRATSFEAAVTTVLAGLDD
                     APAGPEGSPR"
     gene            685..3540
                     /locus_tag="VF720_15015"
     CDS             685..3540
                     /locus_tag="VF720_15015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7880723.1"
                     /translation="MTTELPPDDLPRRLQHAILRTLAESTSLTDATPAVLREICEAFH
                     WDYGGLWLIDPSAGVLRCIETWQSASVVLDEFQRLSRECQFPSGTGLPGRVWKTGQPA
                     WVRDVVEDLNFPRALAADRAGLHGAIGVPIDREGELIGVMEFFSPHVLDPDDRLVQLM
                     TAIGRDLGRFLARKRAMDELEQLFELSLDMFCIADFDGFFRRVNPAWERVLGHPASEL
                     LARPYMEFIHPEDREATVAEASKLLTGESTVQFENRYRHRDGSYRWLSWSARPWARER
                     LIFATARDITERIGAEAELKAAREAALAASRAKGEFLANMSHEIRTPMNAIIGMSDLA
                     LDTELTAEQREYVGAVKDSAESLLRLINDILDFSKIEAGRLQLELTEFAFRDTLADII
                     RVLAPKAHAKGLELACQVSRDVPEWLVGDPVRLGQVLTNLVGNAVKFTEAGEVVVRVE
                     RLAQGRNGVTLEFAVKDTGIGIPREKQQMIFEAFAQADSSTTRMYGGTGLGLGISARI
                     VEMMGGRIDIESEPGAGSTFRFAARFGLKGGRDVRRTPRVSIELHHLPVLVVDDNATS
                     RAITEEMLRTWEMKPVGVAAGPEALQLLRDGAAGGRPFDLVLIDSSMPGMNGYDLAEA
                     VLDEPGLVRGAVMMMISSDRPEGTARCRELGITATVLKPLKPSDVMDALLVALGKRVR
                     SSGRTPKARPVAAPRRELRILVAEDNAVNRMLAERLLARLGHRATVVVNGLEAVSSWE
                     TGAFDLILMDLQMPELDGLEATRRIRAAEKETGLHIPIVALTAHAMAGDRERCLAAGM
                     DDYLSKPLRPEDLVAALERQGAASREPGDVSDAPSSPAETHDESVFDPAELLERVGGH
                     RDLMNRLVGISREELPRMREAVQRAISSGDAKELFQAAHALKGAALNLAASRVAEAAR
                     QLETMGWESALAGAGPVLDELERECDELQRALDARSL"
     gene            complement(3535..4365)
                     /locus_tag="VF720_15020"
     CDS             complement(3535..4365)
                     /locus_tag="VF720_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7880724.1"
                     /translation="MTRILVVDDDRLTRELIEGLLVAEGFEVVTAADGLAAVSEVSRE
                     AFDLIIVDVWMPRMPGLRLLSGLKAMGRMPRAIVMTSDETPEVVLKAIRSNAHRFLRK
                     PVEPKLLLDQVNKVLAQPEALPIDVVSATPEWVELVVPCTEEAADRIQGFLTQLDAGL
                     PDDQRESLGQAFRELLMNAIEWGGRFDPHQRVRISHLRTPRLLLYRIADPGEGFRFEG
                     LEHAAVSNPVEDPVRHVDVRQGMGMRAGGFGILMAREFADEVVYNEAHNEVVLIKYLL
                     "
     gene            4607..5032
                     /locus_tag="VF720_15025"
     CDS             4607..5032
                     /locus_tag="VF720_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880725.1"
                     /translation="MKKRFKVVLGTGVALLSLALIGRATTPPLHPAAGGHDVPGRASF
                     TAEIDPETGQLGVPKGVRPELSAEELNAVDTSDEGLVQVIHADGSASVDLQGRFCSLS
                     MARVGADGKVHIACVDTPELAAKALDPAVPVANVNGEEK"
     gene            5035..7215
                     /locus_tag="VF720_15030"
     CDS             5035..7215
                     /locus_tag="VF720_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HEX7880726.1"
                     /translation="MKKLTPLFAAALLVAGGISNVHARASFQIINADGLNEGFNDPTP
                     AAPVGGNAGTTVGQQRLIAFTFAAEIWSCILDSDVPIVVRAQFNPLTCTTTSGTLGST
                     GSQSIHRDFAGAPLAATWYVASQANALAGMDLNPGVQDMNSSFNSNLGTATCLPTSPW
                     YYGLDENGPAGSTDLVTVLLHEFGHGLGFASTVNRTTGALNSSFIDVYSRHLYDNTQG
                     LHWDAMTNAQRLASITNNNNLVWDGPAVNANGATNLCHEARLTVNSPVVLAGNYVAPL
                     GSMSPCDPNVTADLALIVDNTVPTSDGCETITNNLAGKIAVVDRGTCTFVIKVLAAQN
                     AGAIGVIVCNNAAGAPIEMGGSDPAITIPAVMVSQADGNAIKAEMLNGPVNATIGTNL
                     AALAGADATGHMKMYAPNPSVSGSSTSHWDVSVRPNVLMEPSINADLDHTTDITVHLL
                     KDIGWGFNAAPVAQCQDLHLLAANDCGAVDVAPSQVNNGSFDANGDVLTMTLTPAGPY
                     APGHNAVVFTVSDGCASSSCEADIFVECPVPVRLAAARVAWDGTEALLHWEVEDATDH
                     AGFSVYRSAPGGETTRLTDRLLSGQTRYDYRDRTAPAFGADYFIVELSRSGKETWHGP
                     IALAPASAPPATLTLARITPNPFLYQTRISYSLPKAGNTTVNVYDAQGRVVRHLVAGS
                     QSSGDHQLTWDGSADNGSPAPAGIYFVKLVSGNAVQSQKVMLNR"
     gene            complement(7279..>7880)
                     /locus_tag="VF720_15035"
     CDS             complement(7279..>7880)
                     /locus_tag="VF720_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX7880727.1"
                     /translation="DLFYQVDSSLTRAHGGLGIGLALVKRLVELHGGAVAAYSPGPGK
                     GSEFVVRLPALAPAVPESPPSKGAPKVVTTAYRILVVDDNPDSVDSLALLLKLRGNQV
                     ASAQSGQTAIEMAETFHPDLVLLDIGMPGMSGHEVARHLRTLPRGREMILIALTGWGT
                     DEDRKHSHAAGFDHHLVKPLEPSDLDTVLGYLNGRSPVH"
BASE COUNT         1294 a         2676 c         2718 g         1192 t
ORIGIN      
        1 catgcggcgg gccgagggga acgacgcgga gatcgcgctg gcactcgagg gtgtcggctg
       61 ggcccagctc ctcgggggca acgaccgagc ggcgctcgac acgtttcgcg agttcctcgg
      121 ggcgcaactg gccgccggcg atccggtgat ggtgaaccgc gcgcgggtgg ccgtggccca
      181 ggtggaagtg gcgctcggac acctcgacga ggcgcgcgcg ttgtcgaagg agatcatggc
      241 cttctcgcgc gggcgcggtg atcggcggaa cgagcacttc gcggcccact tcctggcgga
      301 ctgcgacctg atcgaggaga actacgcggc ggcgctccac cgctatcgcg acagcctggc
      361 gctggtgtgg gccatcggcg accggttgga atcgagtttc gaggtgcagg gaagcgccat
      421 gtcgctggcc ggtctcggcc gggccgacga ggcgctccgg ctggccggcg cggtggaagc
      481 cgagtgggaa cgtctcggca tcgagccgca catgggcttc tgggaccggc tgctggaccg
      541 ctacctggtg tcggcccgct ccgccggcgg ggcggcggcg gaggcggcat ggcaatatgg
      601 acgcgccaca tcgttcgaag ccgccgtcac gacggtgctg gccggccttg acgacgcgcc
      661 tgccggaccc gagggatcgc cacgatgaca accgagctac cgcccgacga cctgccgcgc
      721 cgcctgcagc acgcgatcct gcgcaccctg gccgagtcga ccagcctgac cgacgccacg
      781 ccggccgtcc tgcgcgagat ctgcgaggcg ttccactggg actacggcgg cctctggttg
      841 atcgaccctt ccgcgggggt gctgcgctgt atcgagacct ggcaatcggc aagcgtcgtg
      901 ctggatgaat tccaacgtct gagccgggag tgccagtttc cctccggcac gggattgccg
      961 gggcgggtat ggaaaaccgg gcaaccggcc tgggtgcggg atgtcgtgga ggacctcaat
     1021 tttccgcgcg cgctggccgc cgaccgggcg gggctgcacg gggccatcgg cgtgccgatc
     1081 gatcgcgagg gtgaactgat cggcgtcatg gagttcttca gcccgcacgt gctggatccc
     1141 gacgaccgcc tggtccagtt gatgacggcc atcggccgcg acctcggtcg cttcctggcc
     1201 cggaagcgcg cgatggacga actggagcag ctcttcgagc tctcgctcga catgttctgc
     1261 atcgccgact tcgacggctt cttccgccgg gtcaacccgg cctgggagag ggtgctcgga
     1321 caccccgcgt cggaactcct tgccaggccg tacatggagt tcatccaccc cgaggaccgg
     1381 gaggccaccg tggccgaggc atcgaagctg ctgaccggcg agagcacggt gcagttcgag
     1441 aaccgctacc ggcatcgcga cgggtcgtat cgctggctct cgtggagcgc ccgcccgtgg
     1501 gcacgtgagc ggctgatctt cgccacggcg cgcgacatca ccgaacggat cggtgccgag
     1561 gccgagctga aggcggcccg cgaggccgcg cttgccgcga gccgggccaa gggggagttc
     1621 ctggccaaca tgagccacga gatccgtacg ccgatgaacg ccatcatcgg gatgagcgac
     1681 ctggcgctcg acaccgagct caccgccgag cagcgggagt acgtgggggc cgtcaaggat
     1741 tcggcggaga gcctccttcg actgatcaac gacatcctcg acttctccaa gatcgaggcc
     1801 gggcgcctgc agctggaact caccgaattt gccttccgcg acacgctggc cgacatcatc
     1861 cgggtgctgg cgccgaaggc acacgcgaag gggctggagc tggcctgcca ggtgagccgc
     1921 gacgtgcccg agtggctggt gggcgatccg gtccggctcg gccaggtgct gaccaacctt
     1981 gtcgggaacg cggtgaagtt caccgaggcg ggcgaagtgg tggtgcgcgt ggagcgcctc
     2041 gcgcaggggc ggaacggcgt gacgctggaa tttgccgtga aggacaccgg catcgggatc
     2101 ccgcgtgaga agcagcagat gatcttcgag gccttcgcgc aggccgacag ctccaccacg
     2161 cgcatgtacg gcggcacggg gctcggcctc ggcatctcgg cccggatcgt ggagatgatg
     2221 ggggggcgga tcgacatcga gagcgagccg ggcgcgggga gtacgttccg gtttgcggcc
     2281 cgcttcggac tgaagggcgg gcgcgacgtg cgccgcaccc cccgggtctc gatcgagttg
     2341 catcacctcc ccgtgctggt ggtggatgac aacgccacca gtcgggccat caccgaagag
     2401 atgctccgca catgggagat gaagccggtg ggcgttgccg ccggacccga ggcgctgcaa
     2461 ctccttcgcg acggcgcggc cgggggccgg ccgttcgacc tcgtgctcat cgactcgagc
     2521 atgccgggaa tgaacggcta cgacctcgcg gaggcggtcc tcgacgagcc cggcctcgtg
     2581 cgcggagcgg tcatgatgat gatctcgagt gaccggccgg aggggacggc gcgctgtcgt
     2641 gagctcggca tcacggccac cgtcctgaag ccgctcaagc cttccgacgt gatggacgcc
     2701 ctcctcgtgg cactcggcaa gcgtgtccgg agcagcgggc ggacgcccaa ggcgcgaccg
     2761 gttgcggcac cacgccgcga actccggatc ctggtggccg aggacaacgc cgtgaaccgg
     2821 atgctggccg aacgcctcct ggccaggctg gggcatcggg ccaccgtggt agtcaacggg
     2881 ctagaggccg tgtcgtcgtg ggagacggga gcgttcgacc tgatcctgat ggacctgcag
     2941 atgccggagc tcgacgggct cgaagccacg cgccggatcc gcgcggccga gaaggagacc
     3001 ggcctgcata tcccgatcgt ggcgctgacc gcccacgcca tggccggcga tcgcgaacga
     3061 tgccttgccg cgggcatgga tgactacctg tcgaagccgc tacgtccgga agacctggtg
     3121 gcggccctcg agcggcaggg cgccgcgagc cgggaacccg gggacgtgag cgatgcgccg
     3181 tcgtccccgg ccgagacgca tgacgagagc gtgttcgacc cggccgaact gctcgaacgc
     3241 gtcggcggac accgggacct gatgaaccgc ctggtcggga tctcgcggga ggaactcccc
     3301 cggatgcgcg aggccgtaca gcgggcgatt tcttcgggcg acgcgaagga gctcttccag
     3361 gcggcgcacg cgctgaaagg cgccgcgctc aacctggcgg cgagtcgggt ggccgaggcg
     3421 gcgcggcagc tcgaaacgat ggggtgggaa agcgcgctgg cgggggcggg gccggtgctc
     3481 gacgaactcg agcgggagtg cgatgaattg cagcgcgcgc tcgacgcgcg ctcgctatag
     3541 aaggtacttg atcagcacca cctcgttgtg tgcctcgtta tataccacct cgtcggcaaa
     3601 ctcgcgggcc atcaggatgc cgaagccgcc ggcccgcatc cccattccct ggcggacgtc
     3661 gacgtggcgc accgggtctt cgaccgggtt cgagacggcg gcatgctcga gcccctcgaa
     3721 ccggaacccc tcccccgggt cggcgatgcg gtagaggagg aggcgcggtg tgcggaggtg
     3781 gctgatccgg acgcgctgat gcggatcgaa gcggccaccc cactcgatgg cattcatgag
     3841 gagttcgcgg aacgcctgcc cgagcgactc gcgctggtcg tcggggaggc cggcatcgag
     3901 ctgcgtgagg aacccctgga tccggtcggc ggcctcctcg gtgcagggaa cgacgagctc
     3961 cacccattcg ggagtggccg agacgacgtc gatcggcaga gcttcgggct gggccagcac
     4021 cttgttgacc tggtcgagga ggagcttcgg ctccaccggc ttgcgcagga agcggtgggc
     4081 attggaccgg atggccttca agaccacctc gggcgtttcg tcgctggtca tcacgatggc
     4141 gcgcggcatg cgccccatgg ccttgaggcc cgagaggagc ctgagccccg gcatgcgcgg
     4201 catccagaca tccacgatga tgaggtcgaa ggcctccctc gacacctcgg acaccgcggc
     4261 caggccgtcg gccgcggtca ccacctcgaa cccctccgcc acgagcagac cttcgatcag
     4321 ttcacgcgtg agccgatcat cgtcaacgac gagaatccgg gtcattgggg tacctccggc
     4381 agcggcctgc caggatcggc gggaccgcga ttccattcta gccccatttg tccggttcac
     4441 cgaacgcgcg gttcactcgt tttccttgac tggcccggag ggcgctgcca ttctgcgctt
     4501 cctgcagctc cacgttctcc agactatccg gacgcctgac agcgctcctg tgatggaagc
     4561 tccaccgatc gtgccccaca accggtagag gaaggagaag gtccccatga agaagcgttt
     4621 caaagtggtc cttggtaccg gtgtggccct gttgagcctc gcgctcatcg gccgcgccac
     4681 caccccgccg ctgcaccccg cagcaggcgg ccacgacgtt cccggacggg cgtcgttcac
     4741 ggcggagatc gacccggaaa cggggcagct gggtgtgccg aagggggtac gcccggagct
     4801 cagcgccgag gaactcaacg cagtggacac atcggacgag ggactcgtcc aggtgatcca
     4861 tgccgacggc tcggccagtg tcgacctcca gggtcgcttc tgcagcctct cgatggccag
     4921 ggtgggcgcc gacggcaagg tgcacatcgc ctgcgtcgac acgccggagc tggccgcgaa
     4981 ggccctcgac ccggcggttc cggtggccaa cgtgaacggg gaggagaagt gatcatgaag
     5041 aagctcaccc ccctcttcgc cgcggcgctc ctggtggccg gaggcatctc caacgtacat
     5101 gcgcgcgcct cgttccagat catcaacgcg gacggcctga acgaagggtt caacgacccg
     5161 acgccggcgg cgcccgtcgg cggaaacgcc ggcacgacgg tgggccagca gcggttgatc
     5221 gccttcacct tcgcggcgga aatctggagc tgcatcctcg attccgacgt gccgatcgtg
     5281 gtccgggcac agttcaatcc gctgacctgt acgaccacct cgggaaccct gggatcgacc
     5341 ggctcgcagt cgatccaccg cgattttgcc ggggcgccgc tggcggccac ctggtacgtg
     5401 gcatcgcagg ccaatgccct ggccggcatg gacctgaacc ccggcgtcca ggacatgaac
     5461 tcctcgttca actcgaacct ggggacggcc acctgcctcc ccacctctcc ctggtattac
     5521 ggcctcgatg agaacgggcc ggccggttca accgacctgg tgaccgtcct gctgcacgag
     5581 ttcggtcacg gcctcggctt tgcctccacg gtgaaccgca ctaccggcgc gctgaacagc
     5641 agcttcatcg acgtctactc gcgccacctg tacgacaaca cccagggcct ccactgggac
     5701 gcgatgacca acgcccagcg cctggcctcg atcaccaaca acaacaacct ggtgtgggac
     5761 ggcccggcgg tgaatgccaa cggcgccacc aacctgtgcc atgaggcccg gctgaccgtg
     5821 aattcgccgg tggtcctggc cggcaactac gtggcgccgc tcggctcgat gtctccctgc
     5881 gaccccaacg tgacggccga cctcgcgctc atcgtggaca acaccgtccc gacttccgac
     5941 ggctgcgaga cgatcaccaa caacctggcc ggcaagatcg cggtcgtcga ccgtggcacc
     6001 tgcacgttcg tcatcaaggt gctggcggcg cagaatgccg gcgccatcgg cgtgatcgtg
     6061 tgcaacaacg ccgcgggcgc ccccatcgaa atgggcggct ccgacccggc gatcaccatt
     6121 ccggccgtca tggtcagcca ggccgacggc aacgccatca aggccgagat gctgaacggg
     6181 ccggtcaacg ccacgatcgg caccaacctg gcggccctgg ccggcgccga cgccaccggg
     6241 cacatgaaga tgtacgcgcc gaatccatcg gtgtcgggtt cgtcgacctc ccactgggat
     6301 gtcagcgtcc gtccgaacgt gctgatggag ccctccatca acgccgacct cgaccacacg
     6361 accgacatca cggtccacct gctcaaggac atcggctggg gcttcaacgc cgcgccggtg
     6421 gcccagtgcc aggaccttca cctcctcgcc gcgaacgact gcggcgcggt ggacgtggcg
     6481 ccttcgcagg tgaacaacgg ctcgttcgac gccaatggtg acgttctcac catgacgctc
     6541 accccggcgg ggccgtacgc ccccggccac aacgccgtgg tcttcacggt gtcggacggc
     6601 tgcgccagct cgagctgtga agccgacatc ttcgtggagt gcccggtccc ggtccgcctc
     6661 gcggcggccc gcgtggcatg ggacggcacg gaggccctgc tccactggga ggtggaggac
     6721 gccaccgatc acgccggctt ctcggtgtac cgctcggcac ccggcggcga gacgaccagg
     6781 ctcaccgacc gtctcctctc cggccagacc cgctatgact atcgggacag gacggcgccc
     6841 gccttcggcg ccgattactt catcgtcgaa ctctcccgtt cgggcaagga gacgtggcac
     6901 ggtccgatcg cgctggcccc ggcgagtgcg ccgccggcca cgctgacgct ggcccgcatc
     6961 accccgaacc cgttcctcta ccagacccgg atcagctatt cgctgccgaa ggctggaaat
     7021 accacggtca acgtgtacga cgcccaggga cgcgtggtgc ggcaccttgt cgccggcagc
     7081 cagtcctcgg gtgatcacca gctgacctgg gacggcagcg ccgacaacgg cagccccgct
     7141 cccgcaggca tctacttcgt gaagctcgtc tcgggcaacg cggtgcaaag ccagaaggtc
     7201 atgctcaacc ggtagatttc cgccgggacg atcaacagga cgggccgcgg gggttcagcc
     7261 tccgcggccc gttcttcgtc agtgaaccgg cgagcgcccg ttcaggtagc cgagcaccgt
     7321 atccaggtcg gacggctcga gcggcttgac gaggtgatgg tcgaaacccg cggcatgcga
     7381 gtgcttccga tcctcgtcgg tgccccagcc ggtaagggcg atcaggatca tctcccgacc
     7441 gcggggcagg gtgcggaggt ggcgcgccac ctcgtggccg ctcatgccgg gcatcccgat
     7501 gtcgagcagc acgaggtcgg ggtggaacgt ttcggccatc tcgatggcgg tttggccgga
     7561 ctgcgcactg gccacctggt tgccgcggag cttgaggagg agcgccaggc tgtcgaccga
     7621 gtcggggttg tcgtcgacca cgaggatccg gtaggcggtg gtcacgactt tcggcgcccc
     7681 cttcgacgga ggggattcgg gaacggccgg ggccagtgcc ggcagcctca ccacgaactc
     7741 gctccccttc cccggccccg ggctgtaagc cgccactgcc ccgccgtgca gctccaccag
     7801 gcgcttcacg agcgcaaggc cgatccccaa cccgccgtgc gcccgcgtga gggaggaatc
     7861 gacctggtag aagaggtcga
//
LOCUS       DASVOD010000701         8546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_775521, whole genome shotgun sequence.
ACCESSION   DASVOD010000701 DASVOD010000000
VERSION     DASVOD010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8546
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_775521"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1427)
                     /locus_tag="VF720_15040"
     CDS             complement(<1..1427)
                     /locus_tag="VF720_15040"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880728.1"
                     /translation="MTAHPRPLIALALAAGLVPAIVAAPAMLLAAPGGLDPTFGGGTG
                     RIRIGNGPAFEACESMVVQSNGRSVSVGSSQAGTEPAIALIRRMTDGTPDPGFGTGGM
                     ALTRIPGRTIFGHAVALQPDGRIVVAGDFLDGDNFDFLVCRYTTAGVLDPTFGIGGIV
                     VTSLGGDDRADGIAVQPDGRIVVVGGSVAGSNSTFAAVRYLANGTLDPQFGNQGVVLA
                     DIDNSLDDANAVAVLDDGSLILAGSTFNPITAVNRMALLKLTATGALDTSFSIDGIQS
                     TTFGGPNDIGRAVVIQQGTPDKIVVAATSGALGGTSTRFAVARYSLEGKLDYSFDGNG
                     RLTVQPLGFDGGATAVAIQYDPNGTPRILVTGPCASGQLTILRRLDNGDPDNTFDGDG
                     VYLAPPTPGGETGSAVQVFGSRIVAAGTRRRASSSHGEFEELRLLLSGSPDPGFNGEG
                     IRTDAVGCSDLGVVAVAVQPDGRIVA"
     gene            1610..2125
                     /locus_tag="VF720_15045"
     CDS             1610..2125
                     /locus_tag="VF720_15045"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880729.1"
                     /translation="MSPAARRIDPMITELEHESAATKRLLESVPADKLEWRPHPKSMT
                     LGQLANHLAGIPGNISRFLEVDEFDAATVSFTPPQPTARDEILRNFEESLATARSRMN
                     ELDDTRAGGPFRFHRGGVEIFTIPKEAVMRNMLLNHTYHHRGQLTVYLRLLEIPVPAT
                     YGRSADVNPFG"
     gene            complement(2130..3389)
                     /locus_tag="VF720_15050"
     CDS             complement(2130..3389)
                     /locus_tag="VF720_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880730.1"
                     /translation="MTRKPRTSVRLILVALMLANCAASGIARAADPPSDEELADKVKQ
                     TEESVTAMQSILTRLQRTQIGGYVQARAFYSENAVPSSNLWVRRARLNVKNSWDRGQL
                     VLSFDGGTNVVTAKDAYIDWTMVKGEGQRHGLVIRGGQFFRPFGFELERSDSAREFLE
                     RPPTYDVLFPGNRDQGFDLSYGITSALVINGAILNGSGTSTTNLSFRDADDRKDLAAR
                     LRYSFFGPRADIALSYYDGEQVIPAVAAVPAVRGFADSNNNGVQDAGEDTVIVSPAKA
                     GVSAITGSRDRWGVSLNFYDLLGGTLRGEYVGAIDLTTNLASGPRQAEAEVRTWFAQY
                     VHVLPHDFLVGVRYDALDPDVDDTVRLGGDGEQTDLGVVFARTVGDNLTLSLLWEHQQ
                     VNVYDRTAMTTTETSNDPVTFQMQYAF"
     gene            complement(3386..4138)
                     /locus_tag="VF720_15055"
     CDS             complement(3386..4138)
                     /locus_tag="VF720_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880731.1"
                     /translation="MTNGLVADLALSMDHFSLDARLTVSRNETLALLGPNGAGKTTAL
                     DLIAGLRSPDRGRIELDGTVLCDTSSGIDLPPEARRVGLVFQDYALFPHRTVRGNVAY
                     GPVARNLPQSRRAAVTVDCLERLGLTPLADRPVTELSGGQRQRVALARALAADARVLL
                     LDEPFASLDATHRAQVRFELRTFLHESGLPAIVVTHDLGDAVAVADRLVILEAGRIVQ
                     SGTPAEIVEAPTTPFIADLVASMDARSRSRDS"
     gene            complement(4135..4932)
                     /locus_tag="VF720_15060"
     CDS             complement(4135..4932)
                     /locus_tag="VF720_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880732.1"
                     /translation="MMPARTRRTRVTWLDLAAAPLLLFLAVPFLAMLLSLSPGRLLTD
                     LGSPMALKALGLTLITTLAATAIIIVTGTPVAYLLARRTFPGRDWIDTLVDLPITVPP
                     VVGGVALLLAFGRRGLIGEGFDAIGVRIPFSTVAVVMAQVFIASPFFIRAARAGFEAV
                     PERLEASARTLGAGPWHAFWTVTVPLARPALLAGTVLAWARALSEFGATMMFAGNFPG
                     RTQTLALAVMSAMESDLETALAISTLALAFGLAALLLARHWAREALP"
     gene            complement(4943..5719)
                     /gene="modA"
                     /locus_tag="VF720_15065"
     CDS             complement(4943..5719)
                     /gene="modA"
                     /locus_tag="VF720_15065"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX7880733.1"
                     /translation="MKSSIRLLAFATLLTASLVLPAAAETLKIFAAASLTEAFNDIGA
                     LYKEQNPGAEIEFNFAGSQVLRTQIEEGAPADVFVSADHVHMQALQATHLAGDDQVIA
                     TNRLVVVTPMEKARVKRLADLARPGVTVVIANANVPVGRYTMQALARMNMAGLYGDDF
                     QRRFTANVVSQESNVRGVLAKVSLDEADAGVVYLTDARAAAEKVRLIDIPERMNVVAE
                     YPIAVVSASGAKEAAARFIALVQSEAGQAILAARGFLPAR"
     gene            complement(5716..6159)
                     /locus_tag="VF720_15070"
     CDS             complement(5716..6159)
                     /locus_tag="VF720_15070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX7880734.1"
                     /translation="MTNHYTSAQVCALLGISAKTLYLWERSGKIPMPARDRRGWRVFS
                     ERQLVAIRRHAGLLEPNEAAAGKSGAGSRSAGVTKPAISARNQLHGTVVSVSSDGILS
                     EVVLRLPDGQEVVAVVTRSSVRRLGIRKGQGATAIIKATEVMLAT"
     gene            6233..7375
                     /locus_tag="VF720_15075"
     CDS             6233..7375
                     /locus_tag="VF720_15075"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX7880735.1"
                     /translation="MYADAMMRQHAVRFLAALGLTLPAVPAAAAVDHQRAATFRTTIR
                     RDVELRQLVYLPPGYDADTTRAWPLILFLHGSGERGNELDRVATNGLPKELAAGREVP
                     AVVLSPQCPAGVRWEGDLMVEALNALIDDALVRYRIDPRRVYLTGLSMGGFGAWALAA
                     AHPDRFAAVAPVCGAGDPDLAFRMRRLPIWAFHGAKDTVVPVSATQEMADAMARVHGD
                     MRTTIYPEAGHDSWSATYANPELYDWLFQHEKAAEPKIYSLSGATATASSGRASMAID
                     GDVTTRWESEWSDPQWLTIDLGATIAPRRLLLYWETAHGKRYDLLASEDGRTGWTTVA
                     HVTDGDGAIDLIEFPTGFRTRYLKLDMRERGTMWGYSIWEVEVEME"
     gene            7509..8489
                     /locus_tag="VF720_15080"
     CDS             7509..8489
                     /locus_tag="VF720_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880736.1"
                     /translation="MNSHCRTILLLGSLALLLPTRAGAAPYVNDQVAMSEATKRAQAA
                     ALEDFRARQVVYLQGYVERDGTISRWEVRESFAPPAATDSLLVMAKRGDFPWSDAQPR
                     RSGDPGWIIVPITLNTSVPADGPRMSAGQVNQLEALLPGWLSRLRRSDPGLALTQFRH
                     VASHPLNAPSGLFPMNEHFGEPAAARPASLESAPTNSPAFHRLKWEKGLLTRSPDGRY
                     IFDMGADLEVMEDGELGGDCGGGWTIYDGATHQRLWGTTPGNYSHARSGAWAGSDLLA
                     LYGDVRLSHPTNPNLDCYVPAIWIFDFTAKTYTMWEGPAGPQWVFAGRPD"
BASE COUNT         1413 a         2859 c         2928 g         1346 t
ORIGIN      
        1 gccacgatcc ggccgtcagg ctgcaccgcc acggccacga ccccgaggtc cgagcaaccg
       61 acggcgtcgg tgcggatgcc ctcaccgttg aagccgggat cgggcgatcc cgacaggagg
      121 aggcgcagtt cctcgaactc gccgtggctg ctcgaggccc ggcggcgcgt gccggcggcc
      181 acgatccgcg atccaaacac ctgaacggcg ctccccgtct cgccaccggg cgtcggcggc
      241 gccaggtaca cgccgtcccc gtcgaacgtg ttgtcgggat caccattgtc gaggcggcga
      301 aggatggtga gctgtccgct cgcgcacgga ccggtcacga ggatgcgcgg cgtgccgttc
      361 gggtcgtact ggatggccac ggccgtggcg ccgccgtcga acccgagcgg ctgcaccgtg
      421 aggcggccgt tgccgtcgaa ggagtagtcg agtttcccct cgaggctgta gcgcgccacc
      481 gcgaagcggg tggacgtgcc gccgagcgcg ccgctggtgg ctgccaccac gatcttgtcc
      541 ggtgtcccct gctggatcac gaccgcgcgg ccgatgtcgt tcggaccgcc gaacgtggtc
      601 gactggatgc cgtcgatcga gaagctcgtg tcgagcgcgc cggtggcggt gagcttgagg
      661 agcgccatcc gattcacggc cgtgatcgga ttgaacgtgg aaccggccag gatcagcgac
      721 ccgtcgtcga gcaccgcaac ggcgttggca tcgtccagcg aattgtcgat gtcggcgagg
      781 accactccct ggttgccgaa ctgcgggtcg agtgtgccgt tcgccaggta gcggacggcc
      841 gcaaacgtgg agttgctgcc ggccaccgat ccacccacga ccacgatccg gccgtcgggc
      901 tgcacggcga tgccgtcggc gcggtcgtca ccgccgaggg aggtgacgac gatgccgccg
      961 atgccgaacg tcgggtcgag cacacccgcg gtcgtgtagc ggcagaccag gaagtcgaaa
     1021 ttgtcgccat cgaggaaatc gcccgccacg acgatccgtc cgtcgggctg cagcgccacc
     1081 gcgtggccga agatcgtgcg cccgggaatc cgcgtgagcg ccatcccgcc ggtgccgaag
     1141 ccgggatcgg gtgtgccgtc ggtcatgcgc cggatcaggg cgatggccgg ctccgttccg
     1201 gcctgcgatg accccacgct gaccgatcgc ccattggatt gcacgaccat ggactcgcat
     1261 gcctcgaagg ccgggccatt gccgatccgg atccggccgg tcccgccgcc gaaagtggga
     1321 tcgaggccac ccggggccgc caggagcatg gccggcgcgg caacaatcgc cggaacgagg
     1381 cccgcggcga gcgccagggc gatgaggggt cttggatgcg cggtcatggc acctcggcag
     1441 gaatgcaggg ggcgaagtgt gccgacaagg ataccagagg tgccgggagg gccggtgcgg
     1501 ggaggcccgg cggaccgggt cagggaaaac agaatcgtgt cgggcccggg aggctctaac
     1561 ccgtcgcccg cgccccgcat atccgagtca tcaccaccgg aggtccgtca tgagccccgc
     1621 cgcgcgccga atcgacccga tgatcaccga actggagcac gagtccgccg ccacgaagcg
     1681 cctcctcgaa tcggtgcccg ccgacaaact ggagtggcgg ccgcatccga agtcaatgac
     1741 cctcggccag ctggccaatc atctcgcggg aataccgggc aatatctccc ggttcctcga
     1801 ggtggacgag ttcgacgccg cgaccgtctc gttcacgcca ccccagccca ccgcgcgcga
     1861 cgagatactc cggaatttcg aggaaagcct ggcaaccgcc cgctcccgca tgaatgagct
     1921 ggatgacacg cgggccggcg ggccgtttcg attccaccgg ggcggagtcg agatcttcac
     1981 gatcccgaag gaagcggtca tgcgcaacat gctgctgaac catacctatc accaccgggg
     2041 gcagctcacc gtttacctcc ggctgctgga aattcccgtg ccggccacct acggccggag
     2101 tgcggacgtc aacccattcg ggtagacgac tagaacgcgt actgcatctg gaaggtgacc
     2161 gggtcgttgc tcgtctcagt agtggtcatg gcggtcctgt cgtagacatt gacctgctga
     2221 tgttcccaca ggagcgatag cgtgaggttg tcccccacgg tcctggcgaa caccacgccc
     2281 aggtcggtct gctcgccgtc gccaccgagg cgcacggtgt cgtccacatc gggatcgagg
     2341 gcgtcgtagc gcacgcccac caggaagtcg tgcggcagga cgtgcacgta ctgcgcgaac
     2401 caggtgcgca cctcggcctc ggcctggcga gggccggagg cgaggttggt ggtgaggtcg
     2461 atcgcgccga cgtactcgcc gcgcagcgtg ccgcccagga ggtcgtagaa gttgagtgac
     2521 acgccccacc ggtcgcgcga accggtgatg gccgataccc ccgccttggc tggcgacacg
     2581 atcacggtgt cttcgcccgc atcctggacg ccgttgttgt tggagtcggc aaagccgcgg
     2641 acggccggca cggccgccac cgccgggatc acctgctcgc cgtcgtagta cgacagggcg
     2701 atgtcggccc gcggcccgaa gaagctgtag cgaagccgcg ccgccaggtc cttgcgatcg
     2761 tcggcgtcgc ggaatgacag gttcgtggtg gaggtgccgc tgccgttgag gatcgccccg
     2821 ttgatcacga gcgcggacgt gatgccgtac gacaaatcga aaccctggtc ccggttgccg
     2881 ggaaagagga cgtcgtacgt cggcggtcgc tcgaggaatt cgcgcgccga gtcggagcgt
     2941 tccagctcga agccgaaggg acggaagaac tgtccgcccc ggatcacgag cccatgccgc
     3001 tgcccctccc ctttcaccat ggtccagtcg atgtaggcgt ccttggcggt aacgacgttg
     3061 gtgccgccgt cgaacgacag gacaagctgc ccgcggtccc agctgttctt gacgttgagg
     3121 cgggcccgcc gcacccagag gttgctgctg ggcacggcgt tctccgagta gaaggcgcgc
     3181 gcctgcacgt agccgccgat ctgggtccgc tgcaggcggg tgaggatcga ctgcatggcg
     3241 gtgaccgact cttcggtctg cttcaccttg tccgccagtt cctcgtcgct cggcggatcg
     3301 gcggcgcgcg cgatgccgct cgcggcgcag ttggccagca tcaacgcgac gagaatcaac
     3361 cgcacgctcg tgcggggctt tcgagtcatg agtcacggct ccttgaccgg gcgtccatcg
     3421 aagccaccag gtcggcaatg aagggagtag tcggcgcctc gacgatttcg gccggcgttc
     3481 cggactggac gatccggccg gcctccagga tgacaaggcg atcggccacc gccaccgcgt
     3541 cacccaggtc atgcgtgacg acgatggccg gcaacccgga ttcgtgcagg aacgtgcgca
     3601 attcgaagcg aacctgcgca cgatgcgtgg cgtcgagcga ggcgaagggc tcgtccagga
     3661 ggagtacgcg cgcatcggcg gccagcgccc gggccagcgc cacccgctgt cgctgtccgc
     3721 cggaaagttc cgtgacgggt cgatcggcca ggggagtgag gccgagtcgc tcgaggcaat
     3781 cgaccgtcac cgcggcgcgc cgcgattgcg ggaggttccg cgccaccgga ccgtaggcca
     3841 cgttgccgcg aacggtccga tgcggaaaga gcgcgtagtc ctggaatacc agtcccacgc
     3901 gacgcgcttc cggcgggaga tcaatccccg agctcgtatc gcagagaacc gttccatcga
     3961 gttcgatgcg gccccggtcg ggcgaacgca acccggcgat caggtcgagc gccgttgtct
     4021 tgccggcgcc gttggggccg agaagcgcca gcgtttcgtt gcggctcacc gtgaggcgcg
     4081 cgtccagcga gaagtggtcc atcgagaggg cgagatcggc gacgagaccg ttggtcatgg
     4141 cagggcctcc ctcgcccagt gccgggccag gagaagggcg gccagtccga acgccagggc
     4201 cagcgtggag atggcaagcg cggtctcgag atcgctctcc atggccgaca tcaccgccag
     4261 ggccagcgtc tgcgtgcgtc ccgggaagtt gccggcgaac atcatcgtgg cgccgaactc
     4321 ggagagcgca cgagcccacg ccagcacggt gccggccagg agcgccggcc gggccagagg
     4381 caccgtgacc gtccagaagg cgtgccacgg gcctgcaccc agcgtgcgcg ccgaggcttc
     4441 gagacgttcc ggcaccgctt cgaacccggc gcgcgccgcc cggatgaaga aggggctcgc
     4501 gatgaacacc tgcgccatca ccaccgccac cgtgctgaac gggatgcgca cgccgatcgc
     4561 gtcgaaacct tcgccgatga gcccgcgccg cccgaatgcg aggaggagcg ccacgcctcc
     4621 caccaccggc gggacggtga tcggcaggtc gaccagcgtg tcgatccagt cgcggccggg
     4681 aaaggtgcgg cgggccagga ggtaagccac cggcgtgccg gtcacgatga tgatggcggt
     4741 ggcggcgagc gtggtgatga gcgtgaggcc aagtgccttg agcgccatcg gcgagccgag
     4801 gtcggtgagc agtcgtcccg gcgagagaga aaggagcatc gccaggaacg gcaccgccag
     4861 gaagagcagg agcggcgccg ccgccaggtc gagccaggtg acgcgcgtcc gccgggtgcg
     4921 cgccggcatc atgtcgaccc ggtcatcggg ccggaagaaa cccgcgcgcg gccaggatcg
     4981 cctgcccggc ctcgctctgc acgagggcga tgaaccgcgc cgccgcttcc tttgcaccgc
     5041 tcgccgacac gaccgcgatc ggatattcgg ccaccacgtt catgcgctcc gggatgtcga
     5101 tgagccgtac cttttccgcg gccgcgcgcg cgtcggtgag gtacaccacg cccgcatccg
     5161 cctcgtcgag cgacaccttg gccagcacgc cccgcacgtt gctctcctgg ctcaccacgt
     5221 tggccgtgaa gcggcgctgg aagtcgtcgc cgtagaggcc ggccatgttc atcctggcca
     5281 gcgcctgcat cgtgtaacgc cccaccggca cattggcatt ggcgatgacc accgtcacac
     5341 ccggccgggc gaggtcggcg agtctcttca cccttgcctt ttccatcggc gtcacgacca
     5401 ccagccggtt ggtcgcgatc acctggtcgt cgccggccag gtgagtggcc tgcagggcct
     5461 gcatgtggac gtgatcggcc gagacgaaca catcggccgg cgccccttcc tcgatctgcg
     5521 tacgcaggac ctggctgccg gcgaagttga attcgatctc cgcgcccggg ttctgctcct
     5581 tgtagagggc gccgatgtcg ttgaacgctt cggtgaggga ggccgcggcg aaaatcttga
     5641 gggtttcggc cgcggccggg aggacgaggg acgcggtgag gagagtcgcg aacgcgagaa
     5701 ggcggatgga cgatttcatg tggcgagcat gacctccgtg gccttgatga tggccgtcgc
     5761 gccctgcccc tttcggatac ccaggcgtct gaccgagctg cgcgtgacca cagccacgac
     5821 ttcctggccg tccgggaggc gcaggacgac ttccgagagg atgccgtcgg aactgacgga
     5881 gacgaccgtg ccgtgaagtt ggttgcgggc ggagatagca ggcttcgtga cgccggccga
     5941 ccgactgccg gctccggact tgccggccgc ggcctcattc ggttccagga gccccgcatg
     6001 tcgccggatc gcgaccagct gccgctcgga aaagacccgc catccccgcc ggtcccgcgc
     6061 gggcatcggg atctttccgg agcgttccca cagatagagg gtcttggcgg agatgccgag
     6121 gagggcgcag acctgtgcgc tggtgtagtg gttcgtcatg tcgtgagggt atcgcattta
     6181 ccggttagta ccaagtaatt accggaaatg acattctgtc gggcgaaggt tcgtgtacgc
     6241 tgacgccatg atgcgccagc atgccgtccg gttcctcgcc gcccttggcc tcactcttcc
     6301 tgccgtgcca gccgctgccg ccgtcgacca ccagcgcgcc gccacgttcc gcacgacgat
     6361 ccggcgcgac gtcgagctgc gtcagctcgt gtacctcccg ccgggttacg acgccgacac
     6421 cacgcgtgcc tggccgctca tcctcttcct gcacggctcg ggtgaacgcg gcaacgaact
     6481 cgatcgggtg gccaccaacg gccttccgaa ggagttggcg gccgggcggg aagtgccggc
     6541 cgttgtcctt tcgccccaat gcccggcggg cgtgcggtgg gaaggcgacc tgatggtgga
     6601 agccctgaat gccctgatcg atgacgcgct ggtccgctac cggatcgatc cccgtcgcgt
     6661 gtacctcacc ggactcagca tggggggctt cggcgcctgg gcgcttgccg cggcgcatcc
     6721 cgaccgcttc gccgccgtcg cgcccgtgtg tggcgcgggg gacccggacc tggcgttccg
     6781 gatgcgccgc ctcccgatct gggccttcca cggggcgaag gacacggtcg ttccggtgtc
     6841 ggccacccag gagatggccg acgcgatggc ccgcgttcac ggcgacatgc gcacgaccat
     6901 ctacccggag gccggtcacg actcgtggtc ggccacgtat gccaatcccg agctctatga
     6961 ctggctcttc cagcacgaga aggctgcgga gccgaagatt tactcgctgt caggtgcgac
     7021 cgccacggcc tcgagcggcc gcgcctccat ggccatcgat ggcgacgtga ccacccgctg
     7081 ggaatccgag tggagcgacc cccagtggct cacgatcgac ctcggcgcca cgatcgcccc
     7141 gcgccggctc ctgctctact gggagacggc gcacggcaag cgttacgact tgctggcctc
     7201 tgaggacggt cgtacgggct ggacgacggt ggcccacgtc accgacggcg atggcgccat
     7261 cgacctgatc gagttcccga ctggtttcag gacccgttat ctcaagctcg acatgcggga
     7321 gcgtgggacg atgtggggat actcgatctg ggaagtggaa gtggagatgg agtagcgacg
     7381 ggtttgcctg agggtgcggt tatcgcctcc gcgcactgtc ttacaatgta agacacgtgg
     7441 aaattgatgt gccacagcgg cggcactaaa attgacgaag tgaccgaatc ggaacagtat
     7501 cgagggtcat gaattcgcac tgccggacca ttttgctcct gggctcactg gccctcctcc
     7561 tgccgacccg cgcgggcgcc gcgccgtatg tcaacgacca ggtcgccatg tcggaggcta
     7621 cgaagcgagc ccaggcagcc gccctcgagg atttccgggc gcgacaggtc gtctatcttc
     7681 aggggtacgt cgagcgcgac ggcacgattt cacggtggga ggtgcgcgag agcttcgccc
     7741 cgccggccgc cacggattca ctgctcgtga tggcgaagcg cggcgacttc ccgtggtcgg
     7801 acgcgcagcc tcgccggagc ggtgacccgg gttggatcat cgtgccgatc accttgaaca
     7861 cttcggtccc ggcggatggt ccccgcatgt cggccggcca ggtgaaccag ctcgaggcac
     7921 tgctgccggg ctggttgtcg cgactgcggc gcagcgatcc ggggttggcg ctcacgcaat
     7981 tccggcacgt cgcatcccat ccattgaatg cgccatcggg gctcttcccg atgaatgagc
     8041 actttggtga accggcggcg gctcgcccag ccagcctcga aagcgcacct accaacagcc
     8101 ccgcctttca tcggctcaag tgggaaaaag ggctcctgac ccgatcaccc gatggtcgct
     8161 acatcttcga catgggcgcc gacctggagg tcatggagga cggcgagctc ggcggcgatt
     8221 gcggtggcgg ctggacgatc tacgatgggg ccactcatca gcggctgtgg ggcacgactc
     8281 ccggtaatta ttctcacgcg cggagcggcg cttgggcggg ctccgacctt ctcgccctct
     8341 acggcgatgt ccgcctctcg cacccgacca acccgaacct cgattgctac gtgccggcca
     8401 tctggatctt cgacttcacc gcgaagacgt acaccatgtg ggaggggccg gcgggtcccc
     8461 agtgggtatt tgccggccgg ccagactgat ccaaagccca ccaactggtc ccgggaccag
     8521 tcaactggat ccaatccccc ctgtgc
//
LOCUS       DASVOD010000702         4855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_619089, whole genome shotgun sequence.
ACCESSION   DASVOD010000702 DASVOD010000000
VERSION     DASVOD010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619089"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VF720_15085"
     CDS             complement(<1..734)
                     /locus_tag="VF720_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7880737.1"
                     /translation="MTTPLEERWLAHTRYRDPVDLKKLRFLFNAIESVGPAPLDILEV
                     GCGSGNIARPLAWLGHRVLATDIDEASLEFMRIQGVPDTLELKQAGLTELPAGRRFHV
                     IIASEVLEHVDDPARALDVFRGALLPAGLVLVTIPNGRGPWEVGQALSPRRAAGWLAR
                     KTGLYGPLKRALGVRRQEKDAETSTFNFESPHLQHFTMESFEEMVRRAGFTIGVRGHS
                     DGPLTFFPGGRRISWLARADCRLVDRL"
     gene            complement(731..1408)
                     /locus_tag="VF720_15090"
     CDS             complement(731..1408)
                     /locus_tag="VF720_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX7880738.1"
                     /translation="MTPFRLILITDRATARLPLLEAVEAALAGGVDAVQLRDKDLPAD
                     ERYRLGRELSAMVRRHGAQFLVNDRIDLALALRAHGVQLGAASLPIGQARRLLPPECR
                     IGYSAHSPAEAEGAALAGADFVVLAPVYAPGSKSARGGVSDVARPPLPPRCLGAGTVR
                     ATAAALTVPVYALGGITPDRITEVMAPPTPGESSPAGVAVISHMLASQEPGAAARDLA
                     RALGAAS"
     gene            complement(1405..2181)
                     /locus_tag="VF720_15095"
     CDS             complement(1405..2181)
                     /locus_tag="VF720_15095"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX7880739.1"
                     /translation="MTAVLKFGDYEFRSRLIVGTGRYPSVDILEAAIAASGAELVTVA
                     IRRVDLANTAQGSIMGALTRLGVSLLPNTAGCYTARDAVLTAQLGREALGTSLVKLEV
                     IGDEQTLYPDGPALLEAADILIKDGFTVLPYTHDDPVLCRRLAGMGCPAVMPLGAPIG
                     SGLGIRNPYSLRIIRGTCGVPVIVDAGVGTASDASLAMELGCDAVLMNTAIAGARDPV
                     MMARAMRLAVESGRLAFEAGRIAMKLHATASSPIDGIVGR"
     gene            complement(2178..2396)
                     /gene="thiS"
                     /locus_tag="VF720_15100"
     CDS             complement(2178..2396)
                     /gene="thiS"
                     /locus_tag="VF720_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX7880740.1"
                     /translation="MTLDSPLSIQVNGAAVRVPPGTTLAAFLEADGLSGPGIAVAVND
                     AVVRRADLDARVLVEGDRVEIIRAVGGG"
     gene            complement(2500..2703)
                     /locus_tag="VF720_15105"
     CDS             complement(2500..2703)
                     /locus_tag="VF720_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014052398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 domain-containing protein"
                     /protein_id="HEX7880741.1"
                     /translation="MAGAKKSAKKAPAELIISKSRTKAASKKCNVSSEFYGALDKKVR
                     EMIALAESRALGNKRKTLKPVDL"
     gene            3010..3399
                     /locus_tag="VF720_15110"
     CDS             3010..3399
                     /locus_tag="VF720_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880742.1"
                     /translation="MSTRLPLTLLLLAACLAGCGYKKTWVAADPMELTAKPKGYDLLV
                     TREGTQREHRVLGELVVSGRLEPSWSTENSHDRFVDELRKEAIHKGADAVIGLKTSEM
                     DRDGHTEMSVSGQLIIYTAPPPIASRN"
     gene            3492..4022
                     /locus_tag="VF720_15115"
     CDS             3492..4022
                     /locus_tag="VF720_15115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880743.1"
                     /translation="MSRSLAGLMVLSLVLAMPALSGCGASSAPPPPDSAVTPAGQSIE
                     TSGPTVEIYTAVERIVVQGFRLDFPKMRNITNLVGFPPEQWDRVINVPLADLDEIQFR
                     GNIDQATFDRIYKNREQFSVNPAEFFNVQVIRTDGTRADLVAVMPKFRGIKDGTVWEM
                     SMAGNPARIDRIVIRR"
     gene            complement(4009..>4855)
                     /locus_tag="VF720_15120"
     CDS             complement(4009..>4855)
                     /locus_tag="VF720_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880744.1"
                     /translation="LARGLSGGAIDTVQAAWGRNLHPTWSPDATELAFHSDLDGQDDI
                     WRVGVADGGAGPVTASRADDTDPDWSAAANRIVFTSRRSGNEDIWIMDPSGVEYLNLT
                     DDPARDREARWSADGLQVVFVSDRLGQDDVWIVAAAGGEPRRITNDGAHERRPCLLAD
                     DRTIVFESDREGAPALWAVPIEGGLARRLTSPAGAGARDGSPAPVPGDPTRLVFTRST
                     GGNSGVALLSISDGAVTPIVDEPGANEDHPAVTRDGSKVAWQSGGNVDLWTIDASPAR
                     VATGG"
BASE COUNT          800 a         1658 c         1660 g          737 t
ORIGIN      
        1 aggcggtcga cgaggcggca atccgcgcgg gccagccagg aaatccggcg gccgccgggg
       61 aaaaaagtaa gcggcccgtc ggagtgccct cgcacaccga tcgtgaagcc ggcgcgccgg
      121 accatctctt cgaaactttc catggtgaag tgctgcaggt gcggtgattc gaagttgaac
      181 gtcgacgtct cggcatcctt ctcctggcga cgcacgccga gcgcccgctt caagggaccg
      241 tagaggccgg tcttacgggc cagccagccg gccgcccgcc gcggcgaaag cgcctgtccc
      301 acttcccagg gaccgcggcc gttcgggatt gtcacgagga cgagaccggc cgggaggagc
      361 gcaccgcgga aaacgtcgag cgcccgggcc gggtcgtcca catgctcgag cacctcggag
      421 gcgatgatca cgtggaagcg gcgcccggcc gggagctcgg tcagcccggc ttgcttgagt
      481 tcgagcgtgt cgggcacccc ctggatccgc atgaattcga gcgacgcctc gtcgatgtcg
      541 gtagccagca cgcgatgccc gagccaggcc agcggtcggg cgatgttgcc gctgccgcat
      601 cccacttcaa ggatgtcgag cggggccggt ccgaccgatt cgatggcatt gaaaaggaat
      661 cggagtttct tgagatcgac cgggtcgcgg tagcgcgtgt gcgccagcca gcgttcttcc
      721 agcggtgtgg tcacgacgcg gcgccgagtg cccgcgcgag atcgcgggcc gcggcgcccg
      781 gctcctgcga cgccagcatg tgcgagatga cggccacgcc ggccggtgat gactcgccgg
      841 gggtgggggg tgccatcact tcggttattc ggtccggggt gatgccgccg agtgcataga
      901 ccggcacggt gagcgcggcg gctgtggcac gcaccgtccc ggcgccgagg caacggggtg
      961 gaagaggggg gcgagcgacg tccgacacgc cgcccctggc tgatttcgag cccggggcat
     1021 acaccggagc caggaccacg aagtccgcgc cggccagcgc ggctccctcg gcctcagcgg
     1081 gcgaatgcgc ggagtagccg atccggcact cgggcggcag caatcgccgc gcctggccga
     1141 tcggcaggct ggccgcgccg agctggactc cgtgggcgcg gagggccagc gccaggtcga
     1201 tccggtcgtt caccaggaac tgcgcgccat gtcggcggac catcgcggag agttccctcc
     1261 cgagccggta gcgctcgtcg gcagggaggt ccttgtcacg gagctgcacg gcatccacgc
     1321 cgccggccag cgcggcttcg accgcctcga ggagcggaag gcgggcggtg gcccggtccg
     1381 tgatcaggat gagccggaac ggcgtcagcg gccgacgatc ccgtcgatcg ggctggacgc
     1441 ggtggcgtgc agcttcatgg cgatccgccc ggcctcgaag gcaaggcgtc ccgattccac
     1501 cgccagccgc atggcgcggg ccatcatgac cgggtcgcgc gcgccggcga tggccgtgtt
     1561 catgagcacc gcgtcgcaac cgagctccat ggccagcgac gcatccgacg ccgtgccgac
     1621 tccggcgtcg acgatgaccg gcacgccgca cgtcccgcgg atgatccgca ggctgtaggg
     1681 gttccggatg ccgaggcccg atccgatggg agcgccgagc ggcatcacgg ccgggcatcc
     1741 catcccggca agtcgccgac agaggaccgg gtcgtcgtgc gtgtagggaa ggacggtgaa
     1801 accgtccttg atgaggatgt cggcggcctc gagcagggcc gggccgtcgg gatagagcgt
     1861 ctgttcgtcg ccgatgacct cgagcttgac gagtgacgta cccagcgcct cccggccgag
     1921 ctgcgcggtg aggacggcgt ccctggccgt gtagcaacca gccgtgttgg gaaggagact
     1981 cacgccgagg cgcgtcagcg cgcccatgat cgacccctgc gcggtgttgg ccaggtcgac
     2041 ccggcggatg gccacggtga cgagttcggc ccccgacgcg gcgatggcgg cctcgaggat
     2101 gtcgacggag ggatagcggc cggtgccgac gatcagccgc gaacggaatt cgtagtcgcc
     2161 gaatttgagc acggccgtca accccctccg actgcgcgga tgatctcgac gcggtccccc
     2221 tcgacgagca cgcgggcatc gaggtcggcg cgccgcacga ccgcgtcgtt gaccgccacc
     2281 gcgatgccgg gacccgacag gccgtccgct tcgagaaacg cggccagcgt agtgccggga
     2341 ggcacgcgaa ccgcggcgcc gttcacctgg atggaaaggg gagaatcgag agtcatgccg
     2401 tgatggtagc gcacccggcc acgccccaaa agcaagacgg cgaccccgtg ggggccgccg
     2461 tccggcaaaa gctcggcgcc cgtcgggcgc cggtcgatcc tagagatcga cgggcttcag
     2521 ggtcttgcgc ttgttgccga gagcgcgcga ttccgccagc gcgatcatct cgcgcacctt
     2581 cttgtcgagg gcgccgtaga actcgctcga cacgttgcac ttcttcgagg cggccttcgt
     2641 gcggctcttc gagatgatga gttccgcggg agccttcttc gccgacttct tggcaccagc
     2701 catttcgttc actccttgcg tgtagtggtg gcatcgagag gcggccccgc ggccaaaccg
     2761 cgatgttgcc gcccttccaa ccggttcaac gccggggact ataaccgcga caaactccct
     2821 gtcaaagggg cagatggccg taattaaccc ggaaaccccc gtcggaagcg ttttttcacc
     2881 cagccccgat cgacccgact ccgcgcggct tcagtcggga gccgctccgg ccgatggatg
     2941 acaggcacgg accctccgac gccggtgccg gaacgccgtc cccgaaaccc gtttcaaccg
     3001 aggttcgaca tgtcgactcg tctccccctg acgctgctcc tccttgccgc ctgccttgcc
     3061 gggtgcggct acaagaagac ctgggtggcc gccgacccca tggaactgac cgccaaaccg
     3121 aaaggctatg acctgctggt cacccgggaa ggcacgcagc gcgagcaccg cgtgctgggc
     3181 gaactggtcg tgtcggggcg gctggagcca agctggtcca ccgaaaacag ccacgaccgg
     3241 ttcgtggacg aactccggaa ggaagccatc cacaaggggg ccgacgcggt tatcggcctg
     3301 aagacgagcg agatggacag ggacgggcac accgagatgt cggtctcggg tcaactgatc
     3361 atctacaccg cccctccgcc catcgcctcc cggaattgat ctccgcggcc cgaacggttg
     3421 aacggggggc tagcggcccg tagtatccgc ctcgacaaat cccggaattc caataatcga
     3481 ggaggatcgt gatgtcccgc tcgctcgccg gcctgatggt tctgtccctg gtcctcgcca
     3541 tgccagcgct ttccggctgc ggtgccagca gcgccccgcc gcctcccgat tcggccgtca
     3601 ccccggccgg tcagtcgatc gaaacgtcgg gcccgacggt ggagatctac acggctgtcg
     3661 agcggatcgt cgtccagggt ttccgtctcg atttcccgaa gatgcggaac atcaccaacc
     3721 tggtcggatt cccccccgag cagtgggacc gggtgatcaa cgttcccctg gccgatctcg
     3781 acgagatcca gttccgcggc aacatcgacc aggccacctt cgaccggatc tacaagaacc
     3841 gggaacagtt ctccgtgaac ccggcggaat tcttcaacgt gcaggtcatc cgcaccgacg
     3901 gcacacgcgc cgacctggtg gcggtcatgc ccaaattccg aggcatcaag gacggcacgg
     3961 tgtgggaaat gtcgatggcc ggcaacccgg cgcgcatcga ccggatcgtc atccgccggt
     4021 agccaccctc gccggcgagg cgtcgatcgt ccagaggtcc acattgccgc ccgactgcca
     4081 ggccaccttt gatccatccc gggtcaccgc cgggtgatcc tcgttggccc ccggttcgtc
     4141 cacgatcggg gtgacggcgc catccgaaat cgaaagcaga gccacgccgc tgttgccacc
     4201 cgtggagcgg gtgaaaacga gccgggtggg atctccgggt accggtgccg gggagccatc
     4261 ccgggcgcct gcgccggccg gactcgtcaa tcgacgcgcc agccctcctt cgatcggcac
     4321 ggcccagagc gccggggctc cctcccgatc ggattcgaag acgatcgtcc ggtcgtccgc
     4381 cagcagacag ggtcttcttt catgggcgcc gtcattggtg atgcggcgcg gctcgccgcc
     4441 ggcggcagcc acgatccaca cgtcgtcctg cccgaggcga tccgagacga agaccacctg
     4501 gagtccgtcg gcactccacc gcgcctcgcg atccctggcc ggatcgtcgg tcaggttcag
     4561 gtattccacg ccggatggat ccatgatcca gatgtcctcg tttcccgagc gacggctggt
     4621 gaatacgatc cggttggccg cggcggacca gtcggggtcg gtgtcatcgg cccggctggc
     4681 cgtgaccggt ccggcgccgc cgtcggccac gccgacccgc cagatatcgt cctgtccgtc
     4741 gaggtccgaa tggaacgcca gctccgtggc atcgggtgac caggttggat ggaggttgcg
     4801 cccccaggcg gcctgcaccg tgtcgatggc gccgccggag aggccacggg ccagg
//
LOCUS       DASVOD010000703         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_464460, whole genome shotgun sequence.
ACCESSION   DASVOD010000703 DASVOD010000000
VERSION     DASVOD010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464460"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF720_15125"
     CDS             complement(<1..219)
                     /locus_tag="VF720_15125"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX7880745.1"
                     /translation="MARKKIRVAVIGAGAVAQINHIPAYSRLETAEVVAVCDTDLEKA
                     KRVAARFGIPTATANHEELFDRDDIDAVD"
     gene            complement(249..1271)
                     /gene="purM"
                     /locus_tag="VF720_15130"
     CDS             complement(249..1271)
                     /gene="purM"
                     /locus_tag="VF720_15130"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014995248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEX7880746.1"
                     /translation="MKYREAGVDIEAGEAFVNLIKPLVKKTFGPDVMTDLGSFGGVIR
                     VTPAGHDTLLVASIDGVGTKMLLAAEHDRLDDVGRDLVHHCVNDIAVHGARPLLFLDY
                     IGAGRLEPERAARMVAGMADACAAQGAALLGGETAEMPGMYAEGHYDLVGAIVGTVAR
                     ASFVDGSTIAAGDRLIGLASDGLHTNGYSLARRALFETGKHRPDDRPAELGGKRLLDA
                     LLVPHRCYAPSLRAMITAGELKGAAHITGGGIEGNLARILPEGVDAAIDTDSWSIPPL
                     FRIIAAAGPVGEAEMFRAFNMGVGLVVAVGAAHEATALDRLAALGETAFALGRIVSGS
                     GRVRLD"
     gene            complement(1273..2631)
                     /locus_tag="VF720_15135"
     CDS             complement(1273..2631)
                     /locus_tag="VF720_15135"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7880747.1"
                     /translation="MSAVRPSGGAFTAFYLAVVGLGFGLLLVTTPMHPAAFTPILLLW
                     LLLTILTDSNPVHLPNGGYITVASTLDYAGILIFGPVATAWVEVINTLILHGLIQKKP
                     LKKTLFNAASFALTILVAGQVYTMAGGPIGQPPNLVTHGPALLLLGVTYFMVNTGAIS
                     AVIALSTGKRLWAIWRVNFRWTISHMLAAVVVGVALAALYHALGVGGVFLFITPLLLA
                     SYAFRLYTEARRDIIDFVGVLSGVIDEVDPYTHRHTQRVAHYSVLLARRLDLPEQEVE
                     TIEFAALLHDLGKIKQEHRELLLRPAELNAEERRHMASHAEFGASMAARLRSLKATSE
                     IVMAHHEKLDGSGYPRGLKGNEIPLGARIVAVADAFDAMTTDRVYRPAIPLEKALSIL
                     RTDSGTHFDAAVVACLEKLVAEGEIRPGSTGRRVDAVESIAGATPVAPQETPGPTLEP
                     VR"
     gene            complement(2922..>3408)
                     /locus_tag="VF720_15140"
     CDS             complement(2922..>3408)
                     /locus_tag="VF720_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880748.1"
                     /translation="LEAPDPPVIDLVPHLAPASAARRLQFLNLDLSKSPHRTLARVEL
                     ALGTGEAAGEAEGPSTQEESLRLIARATLEAVQRFTGEDSQFALVDLKTIAMGDDTAI
                     LVGIHHQNGRERQMLSGSATAGRDLQTSVVFATLAALNRRLGRLPVREAVEYELRPTL
                     L"
BASE COUNT          560 a         1111 c         1197 g          540 t
ORIGIN      
        1 gtccaccgca tcgatgtcgt cccggtcgaa gagctcctcg tggttggcgg tggcggtcgg
       61 gatgccgaaa cgggcggcca cgcgcttggc cttctcgagg tccgtgtcgc acacggccac
      121 gacctcggct gtctcgagac gcgagtacgc cgggatgtgg ttgatctggg ccaccgcgcc
      181 ggcgccgatg accgccacgc ggatcttctt gcgggccatg agggctcctc gaatcgaagg
      241 ttggtcggtc agtcgagacg gacccggccg gatccagaaa cgatccgtcc gagtgcgaac
      301 gccgtttcgc cgagggcggc gagacggtcc agtgccgtgg cctcgtgcgc cgcgcccacc
      361 gcgaccacga ggccgacccc catgttgaag gcgcggaaca tttccgcctc ccccaccggt
      421 ccggcggcgg cgatgatacg gaaaaggggc ggtatcgacc agctatcggt atcgattgcc
      481 gcatccaccc cctcgggaag gatgcgcgcg aggtttccct cgatgccgcc gccggtaatg
      541 tgcgcggcgc ccttcagctc gccggccgtg atcatggccc ggagcgacgg cgcgtagcag
      601 cgatgcggca cgaggagcgc gtcgagcagg cgctttccac cgagttcggc cggccggtcg
      661 tcggggcgat gcttccccgt ctcgaagagg gcgcgtcgcg ccagcgagta gccattggta
      721 tggaggccgt cggaggcgag gccgatgagg cgatcgccgg ccgcgatcgt ggaaccgtcg
      781 acgaacgacg cgcgggccac ggtgccgacg atggcgccca ccaggtcgta gtgaccctcg
      841 gcgtacattc ccggcatctc ggccgtttcg ccgccaagca gcgccgcgcc ctgcgcagcg
      901 caggcgtcgg ccatccccgc caccatccga gccgcccgct ccggctccag gcgccccgcc
      961 ccgatgtaat cgagaaacag gagcggtcgg gcgccgtgca ccgcaatgtc gttcacgcag
     1021 tgatgcacga ggtcgcgccc cacgtcgtcc aggcgatcgt gctcggcggc caggagcatc
     1081 ttggtgccca caccgtcgat cgaagccacc agcaacgtgt cgtgtccggc cggcgtcacg
     1141 cggatcacgc cgccgaagct tccgaggtcg gtcatcacgt cggggccgaa ggtcttcttc
     1201 accagcggct tgatgaggtt gacgaacgcc tcgcctgcct cgatgtcgac gccggcctcc
     1261 cggtatttca tgtcagcgga ccggttcgag cgtcgggccc ggagtttcct ggggcgcgac
     1321 cggcgtggcg ccggcgatcg attcgacggc gtcgacgcgc cggccggtgg agccggggcg
     1381 gatctcccct tcggccacca gcttctcgag gcaggccacc accgcggcat cgaagtgggt
     1441 gccggaatcg gtccgcagga tcgacagggc cttctcgagg gggatggccg gccggtagac
     1501 gcggtcggtg gtcatggcat cgaaggcatc ggccaccgcc acgatgcggg cgcccagcgg
     1561 gatctcgttc cccttgagac ccctgggata ccccgagccg tcgagctttt cgtggtgcgc
     1621 catcacgatc tcgctggtgg ccttgagcga gcgaagtcgg gccgccatgc tggcgccgaa
     1681 ctcggcgtga ctggccatgt gacgccgctc ctccgcgttc agctccgccg gacgcaggag
     1741 gagctcgcga tgctcctgct tgatcttccc caggtcgtgc aggagcgcgg cgaactcgat
     1801 cgtctccacc tcctgctcgg gaaggtcgag ccgtcgggcc aggagcaccg agtagtgcgc
     1861 cacccgttgg gtgtgccggt gcgtgtaggg atcgacctcg tcgatcaccc cggagaggac
     1921 gcccacgaag tcgatgatgt cgcgccgggc ctcggtgtag agccggaagg catagctggc
     1981 caggaggagc ggcgtgatga agagaaagac gccgcccaca ccgagggcgt ggtacagggc
     2041 ggccagggcc acccccacca ccaccgccgc cagcatgtgg ctgatcgtcc agcggaagtt
     2101 cacccgccag atggcccaga gcctcttgcc ggtggagagg gcgatcaccg ccgaaatggc
     2161 gccggtgttg accatgaagt aggtgacgcc gaggaggagg agcgccggtc cgtgcgtcac
     2221 gaggttgggc ggctggccga tcgggccgcc cgccatcgtg tacacctgcc ccgccaccag
     2281 gatggtgaga gcgaatgacg ccgcgttgaa gagtgtcttc ttcagcggtt tcttctggat
     2341 cagcccgtgg aggatgagcg tgttgatcac ctccacccag gccgtggcca ccggaccgaa
     2401 gatgaggatg ccggcgtagt cgagcgtgga ggccaccgtg atgtagccgc cgttgggaag
     2461 atgaacggga ttcgaatcgg tgaggatggt gaggaggagc cagaggagca ggatgggcgt
     2521 gaaggccgcc gggtgcatcg gcgtggtgac gagcaggagc ccgaatccca ggccgacgac
     2581 ggcgaggtag aaggcggtga acgcgccacc cgatggacgt acggcgctca tgttccgcta
     2641 cctcggccag atcgccgggg ctcgtgcgag ccgcggcgca aaattaatgg gaccgacatc
     2701 gaggggggag tattaccagc ctgccttacc cggcaggacg ctgagtgcga cactcagcac
     2761 cagagcgagc catccccact tcatattgga acccaccttt cggtactgga gaaacgggtg
     2821 caaaaacagc cagttgatgg gttcgcttcg ctcgaagact tctgtaacgc ttcgctgtcc
     2881 cccggtcgat gtcggtcggt gtaccggatt ggagtgccac gctaaaggag cgtggggcgg
     2941 agttcgtact caaccgcctc ccgaaccggg agccgaccaa ggcgccggtt cagggctgcc
     3001 aaggtagcaa ataccaccga cgtctgtaaa tctcggccgg ccgtggccga gccggacagc
     3061 atctggcgtt cccggccatt ctggtggtgg atgcccacca ggatcgccgt atcgtcgccc
     3121 atggcaatgg ttttcaggtc gaccagggca aactggctat cctcccccgt gaagcgctgg
     3181 acggcttcca gggtggctcg cgcgatcagc cggagcgatt cttcctgggt gctcggccct
     3241 tcggcctccc cggccgcctc cccggtcccg agggccagct ccacgcgggc cagggtgcga
     3301 tgggggctct tcgagaggtc gaggttcagg aactggagcc gccgtgcggc cgacgccggc
     3361 gccaggtgcg gcaccaggtc gatgacgggc ggatcgggag cctcgagc
//
LOCUS       DASVOD010000704         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_202779, whole genome shotgun sequence.
ACCESSION   DASVOD010000704 DASVOD010000000
VERSION     DASVOD010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202779"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF720_15145"
     CDS             complement(<1..357)
                     /locus_tag="VF720_15145"
                     /inference="COORDINATES: protein motif:HMM:NF045460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX7880749.1"
                     /translation="MATGGIMLTGTNVAGRHLLVVGLARSGVAAARLLLRHGARVSGV
                     DARSLEALGDDVRALAGEGVTLHAGDGRLAILDGVDALVVSPGVPRSVPLVAEAATRG
                     LTILGELELVARFSKAP"
     gene            complement(402..1484)
                     /gene="mraY"
                     /locus_tag="VF720_15150"
     CDS             complement(402..1484)
                     /gene="mraY"
                     /locus_tag="VF720_15150"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX7880750.1"
                     /translation="MFLWLLYPLHEDFSFLNVIRYITFRSAYAGVTALVLSFVLGPPI
                     IRWLSQVRMRQIIRRDGPTSHYTKEGTPTMGGLIILAAIVIPTILWGNLENRLVLIAL
                     IATVWLGTIGFLDDWLKIQRVPKGLLGRYKLVAQGALGLGIGTYLYFNPVHPTAATIT
                     TIPFLKNFFLDLGIFYIPFVAIVITGASNAVNLTDGLDGLATGTAAMAAVAFAGLAYL
                     SGHARFSEYLNILFVREAGELTVFLAAMVGAALGFLWFNTHPAQVFMGDTGSLALGGA
                     LGTVAVLIKREFLLVVVGGIFVLEALSVIVQVASYKLRKKRILKMAPLHHHFELSGWA
                     ETKVVVRFWIVAALFALLSLSTLKLQ"
     gene            complement(1487..>2235)
                     /locus_tag="VF720_15155"
     CDS             complement(1487..>2235)
                     /locus_tag="VF720_15155"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HEX7880751.1"
                     /translation="AADTLIYLGDDSLLREMNRQRIAHVVTFGLGSTPNLTASNILEK
                     GLAGSEFDAEGFGRVTLAIPGRHNVLNALAALAVSRTLGVPPGDAVAALARVRPFSGR
                     MEVRRFGAITVIDDSYNANPDSMRAALDLLRETPHAGRRVAVLGEMLELGQATVPMHR
                     EVGAAASFVDRLFLIGPSAGEVAKAAQGAGLAPDRIREFPHLDILLATVPGALQDGDL
                     VLVKGSRGMALDRLVSAIESRAGHPAGGGR"
BASE COUNT          408 a          778 c          740 g          309 t
ORIGIN      
        1 cggcgccttg ctgaagcgag cgaccagctc gagctccccc aggatcgtga ggccgcgcgt
       61 cgcggcctcc gccacgagcg gcaccgagcg gggcacaccc ggcgagacga ccagcgcgtc
      121 cacgccgtcg aggatcgcaa ggcgaccgtc gccggcgtgc agcgtcacgc cttcgcccgc
      181 cagcgcccgg acatcgtcgc ccagggcctc gagcgacctg gcgtcgacgc ccgacacgcg
      241 cgcgccatgg cggaggagga ggcgcgccgc ggccacgccg ctccgcgcca gccccaccac
      301 caggaggtga cgaccggcca cgttggtgcc ggtcagcatg atgccgcccg tcgccacgtc
      361 cggctgtcgt tccgcacagc agtcatcgat cgccccgacc gtcattgaag tttgagggtg
      421 gacaaagaca gaagcgcaaa aagcgccgcc acaatccaga accgcaccac caccttcgtc
      481 tccgcccacc ctgacaattc gaagtggtgg tgcagcgggg ccattttcag gatgcgcttc
      541 ttgcgcagct tgtacgaggc cacctgcacg atcaccgaca gcgcttcgag cacgaagatg
      601 ccgccgacca ccaccaggag aaattcgcgc ttgatgagga ccgccacggt gcccagggcg
      661 ccgcccaggg ccaggcttcc cgtatcgccc atgaagacct gggcgggatg tgtattgaac
      721 cagaggaatc cgagcgccgc gccgaccatg gccgccagga acacggtcaa ttcgccggct
      781 tcgcgcacga agaggatgtt caggtactcg ctgaagcggg cgtgcccgga gaggtaggcc
      841 agccccgcga acgccaccgc cgccatggcg gccgtcccgg tggcgagacc gtccaggccg
      901 tcggtgagat tcaccgcgtt cgaggccccg gtgatcacga tcgccacgaa cgggatgtaa
      961 aaaatcccca ggtccagaaa gaaatttttc aggaatggga tggtcgtgat cgtggcggcc
     1021 gtcgggtgga ccggattgaa atacagatag gtcccgatcc cgaggccgag agccccctgc
     1081 gccaccagct tgtagcgccc caggagtccc ttcggcaccc gctggatctt gagccagtca
     1141 tcgagaaaac cgatcgtacc cagccagacg gtggcgatca gggcgatcag cacgagccgg
     1201 ttctcaagat ttccccacag gatcgtgggg ataacaatgg cggccaggat gatgaggccg
     1261 cccatcgtcg gcgtcccttc cttggtgtag tggctggtgg ggccgtcccg gcggatgatc
     1321 tgccgcatgc gcacctggga gagccaccgg atgatcggcg gccccagcac gaacgagaga
     1381 acgagcgctg tcacgccggc gtaggccgag cggaacgtga tgtagcggat cacgttgagg
     1441 aacgagaagt cctcgtgcag cgggtagagg agccagagga acacgctcag cgccccccgc
     1501 cggccggatg ccccgcgcgc gactcgatgg ccgaaaccag ccggtcgagt gccattccac
     1561 gcgatccctt caccaggacc aggtcgccgt cctggagcgc gcccggcacg gtggccagca
     1621 ggatgtcgag atgggggaat tcccgaatgc gatccggagc gagcccggct ccctgggccg
     1681 ctttcgccac ctcgccggcc gaggggccga tcaggaagag gcggtcgacg aagctcgcgg
     1741 ccgcccccac ctcccggtgc atcggcacgg tggcctgccc cagctcgagc atctccccga
     1801 gcaccgccac gcgccgcccg gcatgcggcg tctcgcgcag gagatcgagc gcggcccgca
     1861 tcgaatcggg attggcgttg tagctgtcgt cgatgacggt gatcgcgccg aagcgccgca
     1921 cttccatccg accgctgaag gggcgcacgc gggccagcgc ggccacggca tcacccggcg
     1981 ggacgccgag ggtgcgactc accgccagcg cggccagcgc attcagcacg ttgtgcctcc
     2041 ccgggatggc cagcgtcacg cggccgaatc cctccgcgtc gaactcgctc ccggccagcc
     2101 ccttctcgag gatgttggat gcggtcaggt tcggcgtcga accgagcccg aacgtgacca
     2161 cgtgcgcgat ccgctgcctg ttcatttccc ggaggagcga atcgtcaccc aggtagatga
     2221 gcgtatcggc cgcgg
//
LOCUS       DASVOD010000705         4475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_203005, whole genome shotgun sequence.
ACCESSION   DASVOD010000705 DASVOD010000000
VERSION     DASVOD010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203005"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF720_15160"
     CDS             complement(<1..375)
                     /locus_tag="VF720_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotolerance regulator BatB"
                     /protein_id="HEX7880752.1"
                     /translation="MRWGDPIFLKLLPGLIVLIILAVIGASQAARRARKMADDALFHR
                     VAPLVPGWRRALLVALPLLALAASLLGAARPQLGSRKVQVKRSGVDLVMALDTSASMD
                     AADVVPNRLARARREMIDLIDQL"
     gene            complement(372..1382)
                     /locus_tag="VF720_15165"
     CDS             complement(372..1382)
                     /locus_tag="VF720_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX7880753.1"
                     /translation="MNLLPHDFRFAEPLFLLLLLFIPALLALRWWRRRHAPSVLWADA
                     GRWMGGGSSWRTVADRLLAVLPWLAFALLVVAMARPQSGTREMDIKSEGIDIIAVIDA
                     SGSMRAEDFQPNNRLFVAKQVAGQFVEGRDGDRIGVVVFAGEAFTQCPLTLDQGVLQD
                     LIKSIDFGIEPDGTAIGSAIATAVNRLRESQAKSKVVVLLTDGRNNSGSVDPITAADA
                     AAALGIKIYTIGVGVQGEAPYPVDDPLFGRRYVRMPSDVDDATLTTIATKTGGLYFRA
                     TSAEALKEIYDRIDRLEKTTVESREYIDYADLGPQILAWGSLLLALLVLGRATLVTRL
                     PA"
     gene            complement(1379..2443)
                     /locus_tag="VF720_15170"
     CDS             complement(1379..2443)
                     /locus_tag="VF720_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880754.1"
                     /translation="MSAPRRLLATVWLLMTLWATPLLAQGEAVGLPGGAEQRVSPSVV
                     RIGDSIHVSVLAPSGAVLALPALSDSLGPFMVLGSEIGNRDGRSLLAVHLAAFSPGSL
                     EIPSFDVPVAGGLAIATRPYRITVQSLLPADSAAVDSMTIRAAHGPLDLPQEFRWKVA
                     LGYLLVLAALGALAWYLYRRWKNRPIVAPVVPEAPAVVRPADAVALEALDAVMAKGYA
                     LRGLFKEHYTETLDVLRVFIEQRLRVEAMDRTSFELLGEMKRANAGARAISGLATLLD
                     EAELVKFARQTPTVESAVALVDAARKWVKETHAEATAREALVMSPATPVVAPAATASS
                     TPFTAPTTPTSPPPPAEGAR"
     gene            complement(2440..3315)
                     /locus_tag="VF720_15175"
     CDS             complement(2440..3315)
                     /locus_tag="VF720_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018053208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX7880755.1"
                     /translation="MIPREVLRQVRRIEIRTRRLVNDVFGGEYHSVFKGRGMEFAEVR
                     EYVPGDDIRAIDWNVTARFGQPFIKIFSEERELTVVLLVDVSASGDFGTRGRLKADLA
                     AEVGAVLAFSAVTNNDKVGLILFTDEVEQHVPPRKGKRHVLRVIREILYFKPKRRGTR
                     ISAALEHLARVQKRKAVVFLISDFETEGYEPAFKLAAKRHDLVAIRISDPHERTLPNV
                     GLVMMEDPETGRRQLVDTSSKKVRARFAERRAERDAAFRQARKEAQVEAIELTTDEPY
                     AKPLIQFFAGREKKR"
     gene            complement(3362..4345)
                     /locus_tag="VF720_15180"
     CDS             complement(3362..4345)
                     /locus_tag="VF720_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7880756.1"
                     /translation="MFDVVALNERVKAESASLSRLTDEVARVIVGQKYMVERLMISLL
                     ADGHVLLEGVPGLAKTTAIKTLASAIDTGFARVQFTPDLLPADLTGTQVYNPKDASFS
                     VKQGPVFTNLLLADEINRAPAKVQSALLEAMQERHVTIGGSTFKLPEPFMVLATQNPI
                     EQEGTYPLPEAQVDRFMLKLNVGYPTREEERDILERQTGAVPAPVQPVIHPEQILRMR
                     QVVREIYMDDKIKKYIVDIVFATREPEAAGLDLKPLIAFGASPRATIYLAQAARAHAA
                     LRGRGYVTPEDVKAIGLDVLRHRVIVTYEAEAEEVTPEEVARRILDKVEVP"
BASE COUNT          715 a         1489 c         1544 g          727 t
ORIGIN      
        1 caactggtcg atcaggtcga tcatctcccg gcgcgcccgc gccaggcggt tgggcaccac
       61 gtcggcggca tccatgctgg ccgaggtgtc gagcgccatc accaggtcca cgccgctccg
      121 cttgacctgc accttgcgcg agccgagctg cggccgcgcc gcgccgagca gcgaagcggc
      181 cagcgcgagg agcggcagcg ccacaagcag tgcccggcgc cagccgggca ccagcggcgc
      241 cacccggtgg aacagcgcgt catcggccat cttccgggcc cgacgggccg cctgggacgc
      301 gccgattacc gcgagaatga tcagcacgat gagccccggg aggagcttca ggaaaatcgg
      361 atcgccccat ctcatgccgg caacctcgtg acaagcgtgg cccgccccaa tacaagcagg
      421 gccaggagaa ggcttcccca cgccaggatc tgcggcccca ggtcggcata gtcgatgtat
      481 tcgcggctct cgaccgttgt tttctcgagc cgatcgatgc ggtcatagat ttccttgagc
      541 gcctcggcgg aggtggcccg gaagtaaagg ccgccggtct tggtggcgat cgtggtcagc
      601 gtggcgtcgt ccacgtcgga gggcatgcgg acgtagcgcc ggccgaagag cgggtcatcg
      661 accggatagg gcgcctctcc ctgcacgccg acgccgatcg tgtagatctt gatgccgagc
      721 gccgccgccg cgtcggccgc cgtgatcgga tcgaccgagc cggagttgtt gcgcccgtcg
      781 gtgaggagca caaccacctt gctcttggcc tggctctcgc gcaaccggtt caccgccgtg
      841 gcgatggcac tgccgatggc ggtgccgtcc ggctcgatgc cgaagtcgat cgacttgatc
      901 agatcttgta atacgccctg gtcgagcgtg agcgggcact gggtgaacgc ctcccccgca
      961 aagaccacca cgccgatccg gtcgccgtcg cgtccctcga cgaactggcc ggccacctgc
     1021 ttggccacga agaggcggtt gttcggctgg aagtcctcgg cgcgcatcga gcccgaggca
     1081 tcgatcacgg cgatgatgtc gatcccctcg ctcttgatgt ccatctcgcg cgtgcccgac
     1141 tgggggcgcg ccatcgcgac cacgaggagc gcgaacgcca gccacggcag gacagccagc
     1201 aggcgatcgg ccaccgtgcg ccagctcgat ccgcccccca tccagcgtcc ggcatccgcc
     1261 cagaggacgc tcggcgcatg acggcgccgc caccagcgaa gggcgagcag cgcagggatg
     1321 aagaggagca gcagcaggaa gagcggttcg gcgaaccgga agtcgtgcgg caggaggttc
     1381 atcgggcccc ttccgcgggt ggcgggggtg acgtcggtgt ggtcggcgcg gtgaacggcg
     1441 tgctcgaagc ggtggccgcc ggcgccacca ccggtgtcgc cggtgacatg acaagcgcct
     1501 cgcgcgccgt cgcttccgcg tgggtttcct tcacccactt ccttgccgcg tcgaccagcg
     1561 ccacggcgga ctcgaccgtc ggcgtctgcc gggcaaactt caccagctcg gcctcatcga
     1621 ggagcgtggc caggcccgag atcgcgcgcg cgccggcgtt ggcgcgcttc atctcgccca
     1681 gcagctcgaa cgaggtgcga tccatggcct ccacgcggag ccgctgctcg atgaagaccc
     1741 ggaggacgtc gagcgtttcg gtgtaatgct ccttgaagag cccgcgcagc gcgtacccct
     1801 tggccatgac ggcgtcgagc gcctcgagag ccacggcatc ggcggggcgc acgaccgccg
     1861 gcgcttcggg aacgaccggc gccacgatcg ggcggttctt ccagcgccga tacaggtacc
     1921 acgccagcgc gccgagcgcg gccaggacga gcaggtagcc gagggccacc ttccatcgga
     1981 attcctgcgg aaggtcgagc ggcccatggg cggcccggat ggtcatcgaa tcgacggcgg
     2041 cggagtccgc cggcaggagg ctctgcaccg tgatccggta cgggcgcgtg gcgatcgcca
     2101 ggccgccggc aaccggaacg tcaaacgagg ggatctcgag cgagccgggg ctgaaggcgg
     2161 ccaggtgaac cgccaggagc gagcggccgt cgcggttgcc gatttccgag ccgaggacca
     2221 tgaagggccc cagcgagtcg gagagcgccg gcagcgcgag aaccgcgccc gagggagcca
     2281 gcacactcac gtggatcgaa tcgccgatgc gcacgacgct gggcgatacg cgctgctcgg
     2341 caccgccggg cagaccgacg gcttccccct gggcgaggag cggcgtggcc cacagtgtca
     2401 tcaacagcca cacggttgcg aggagtcgtc gaggcgcgct catcgcttct tctcccggcc
     2461 ggcaaagaac tggatcagcg gcttggcata cggctcgtcg gtggtgagtt cgatggcctc
     2521 gacctgggct tccttgcgcg cctgccggaa cgcggcgtcg cgctcggccc gtcgttcggc
     2581 gaaacgggcc cggacttttt tcgaggaggt atcgaccagc tggcggcgcc ccgtttccgg
     2641 atcttccatc atcacgaggc ccacgttcgg gagcgtccgt tcgtgcggat cgctgatccg
     2701 gatggccacg aggtcgtgcc gtttggctgc gagcttgaag gccggctcgt atccctcggt
     2761 ctcgaagtcg gaaatgagga agacgaccgc cttccgtttc tgcacgcgcg ccaggtgctc
     2821 gagcgccgca ctgatccgcg taccgcgccg cttcggcttg aagtagagga tctcgcggat
     2881 gacgcggagg acgtgacgct ttcccttgcg gggcggcaca tgctgctcca cctcgtcggt
     2941 gaagaggatg aggccgacct tgtcgttgtt ggtgacggcg ctgaaggcga gcacggcgcc
     3001 cacctcggcg gccagatccg ccttgaggcg gccgcgcgta ccgaaatcgc ccgaggccga
     3061 cacgtcgacc aggagcacga cggtaagctc tcgctcttcc gagaagatct tgatgaaggg
     3121 ctggccgaaa cgcgcggtga cgttccagtc gatggcgcgg atgtcgtcgc ccggcacgta
     3181 ttcgcgcact tcggcgaact ccatcccccg tcccttgaag acggagtggt actcgccgcc
     3241 gaagacatcg ttgaccagtc gccgggtccg gatctcgatc cgtcggactt ggcggaggac
     3301 ttcgcgtggg atcatcgttt tccgttacgt gagctcggga atcgcggccg cacggccgtg
     3361 atcaggggac ttcgaccttg tcgaggatcc ggcgcgccac ttcctcgggc gtcacctctt
     3421 ccgcttcggc ctcgtaggta acgatgacgc gatggcggag cacgtcgagc ccgatggcct
     3481 tcacgtcctc cggcgtcacg tacccgcgcc cgcggagcgc cgcgtgcgcg cgggccgcct
     3541 gcgccaggta gatcgtggcc cggggggagg cgccgaacgc gatgagcggc ttcaggtcga
     3601 gccccgccgc ctccggctcg cgcgtggcga agacgatgtc gacgatgtac ttcttgatct
     3661 tgtcgtccat gtagatctcg cggaccacct ggcgcatccg gaggatctgt tccggatgga
     3721 tgaccggttg gacaggcgcc ggcaccgcac ccgtctggcg ctcgaggatg tcccgctcct
     3781 cctcgcgcgt cgggtagccg acgttgagct tcagcatgaa gcggtcgacc tgcgcttccg
     3841 gcagcggata cgtcccttcc tgctcgatcg ggttctgcgt ggccagcacc atgaacggct
     3901 cgggaagctt gaaggtgctc ccgccgatcg tgacgtgccg ttcctgcatg gcctcgagga
     3961 gggccgactg caccttggcc ggcgcgcggt tgatttcgtc ggccaggagg aggttcgtga
     4021 agaccggacc ctgtttcacg ctgaaggagg cgtccttcgg gttgtagacc tgagttccgg
     4081 tcaggtcggc gggaaggagg tcgggcgtga actgcacccg ggcgaagccc gtgtcgatgg
     4141 cggaggcaag ggtcttgatg gccgtcgtct tggccaggcc cggcacgccc tcgaggagta
     4201 cgtgcccatc ggcgagcagc gagatcatga gccgctccac catgtatttc tggcccacga
     4261 tgacccgagc cacctcgtcg gtcaggcggg agagcgatgc gctctcggcc ttgacccgct
     4321 cattcaacgc cacgacatca aacatccgtg gtacgacctc cgtaacctgt tgtgaattca
     4381 gttgaaagcg gccggtgttc aagtcccgcc ggggttggcg ggttcccgag gataccggga
     4441 ccggctgttc ctctcaacct agacgggagt gactg
//
LOCUS       DASVOD010000706         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_880965, whole genome shotgun sequence.
ACCESSION   DASVOD010000706 DASVOD010000000
VERSION     DASVOD010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_880965"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VF720_15185"
     CDS             <1..1044
                     /locus_tag="VF720_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX7880757.1"
                     /translation="PGLHLPVSELNELRRRLVAELDATSRAGELARRDVPLAPEGVAR
                     RLIGDPRTAAAPESRSARLVPLCRTDAQLDAAIAAGCPEVELDWMELVGLERAVARAR
                     EAKLTVTIATVRVQKPGEEGYDRRLARLKPDGLLVRHWGALESFRSSGAADPAAPVLH
                     GDFSLNVTNAVTARFLLSRGLADVTPAHDLDVVQLGRFLDHVDPSRVTVVLHHHIATF
                     HTEHCVYAHLLSEGRDFRDCGRPCEEHLVALRDRTGDEHPVVVDVGCRNTVFNARAQT
                     AARHVPALLTRGVRRFRAEFVREGREETAEVLAAYRALLDGASPPDDAVRRVGALERF
                     GVTSGTLRVMDPA"
     gene            complement(1055..1858)
                     /locus_tag="VF720_15190"
     CDS             complement(1055..1858)
                     /locus_tag="VF720_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX7880758.1"
                     /translation="MNTLTLSLLPVLFLVGLIILGNSVRVLREYERAVVFRLGRLIGV
                     KGPGLILLLPMIDRMVKVPLRVVAMDVPAQDIITKDNISIKVNAVLYFKVTSAQEAVI
                     EVENYLYATSQIAQTSLRSVVGEHELDDLLAERDKINIKLQQIIDERTEPWGVKVTAV
                     EVKDVDLPQEMQRAIARQAEAERERRAKIIHAEGEYTASLQLAAAAGRMSAEPLAIQL
                     RYLQTLTEIATEKNSTIVFPLPIDLIKVFLPADAAKRLETTTPVPPPVA"
     gene            complement(1855..>2157)
                     /locus_tag="VF720_15195"
     CDS             complement(1855..>2157)
                     /locus_tag="VF720_15195"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEX7880759.1"
                     /translation="VMLPAVVVTAAFFLFLVGKGLAAQRRPAATGAAAMIGRRGVAAT
                     KLAPTGSVFMDGTHWEAEAPHDSIVDAGQAVRVVAVDRLSLKVRPDSPSPAAGETR"
BASE COUNT          322 a          733 c          759 g          343 t
ORIGIN      
        1 cccggcctgc acttgccggt gtcggagttg aacgaactcc ggcgacggct cgtggcggag
       61 ctcgacgcca cttcccgggc cggggagctg gcccgacgcg atgtgccgct cgccccggaa
      121 ggcgtggcgc ggcggttgat cggtgatccc cggacggcgg cagcgcccga atcccgatcc
      181 gcccgcctcg ttcccctctg ccgcaccgac gcgcagctcg acgcggcgat cgccgccggt
      241 tgccccgaag tcgaactcga ctggatggag ctggtggggc tcgagcgggc cgtcgcaagg
      301 gcacgcgagg cgaaactgac ggtgacgatc gccacggtga gggtgcagaa gccgggcgaa
      361 gagggctacg accggcgcct ggcccgcctc aagcccgacg ggctcctggt ccggcactgg
      421 ggcgccctcg agtctttccg gtcgtccggc gccgcggatc cggcggcgcc ggtgctgcat
      481 ggcgatttca gtctcaacgt gaccaacgcg gtcacggccc gattcctcct gtcccgggga
      541 ctcgccgacg tgacaccggc ccacgacctc gacgtcgtgc agctcggacg cttcctcgac
      601 catgtcgatc cgtcgcgggt caccgtggtg ctgcaccatc acatcgccac gttccacacc
      661 gagcattgcg tctacgcgca tctcctgtcg gaagggcgcg acttccgtga ctgcggccgc
      721 ccgtgcgagg agcacctggt ggcgctgcgc gaccggaccg gagacgagca cccggtggtc
      781 gtggacgtcg gctgccggaa caccgtcttc aacgcccggg cgcagaccgc cgcccgccat
      841 gtacccgcgc tccttacccg gggagtccga cggttccgcg ccgagttcgt gagggagggg
      901 cgcgaggaga ccgccgaagt cctcgcggcc taccgggcgt tgctcgacgg cgcctcgccg
      961 ccggacgacg ccgtccggcg ggtcggggcg ctcgaacgat tcggcgtgac aagcggcact
     1021 ctccgggtca tggacccggc ctgaaccgcc ccggctaggc caccggggga ggaaccggcg
     1081 tcgtcgtctc cagccgcttc gccgcatcgg cgggcaggaa caccttgatc aggtcgatgg
     1141 ggagcgggaa gacgatcgtg gaattctttt cggtggcgat ctcggtcagc gtctggagat
     1201 agcggagctg gatggccagc ggttcggcgc tcatccggcc ggccgccgcc gccagctgga
     1261 gcgaggccgt gtattccccc tccgcatgga tgatcttggc ccgccgttcg cgctcggcct
     1321 cggcctggcg tgcgatggcc cgctgcatct cctgaggcag gtcgacatcc ttcacctcca
     1381 cggccgtcac cttgaccccc cagggctcgg tgcgttcgtc gatgatctgc tgcagcttga
     1441 tgttgatctt gtcgcgctcg gcgagaaggt cgtcgagctc atgctcgccg accacgctcc
     1501 gcagcgaggt ctgggcgatc tgcgacgtgg cgtagaggta gttctcgacc tcgatcacgg
     1561 cctcctgcgc cgatgtcacc ttgaagtaga ggaccgcgtt gaccttgatg gagatgttgt
     1621 ccttcgtgat gatgtcctgc gcggggacat ccatcgccac gacccggagc ggcaccttca
     1681 ccatgcggtc gatcatcggc aggaggagga tcaatcccgg ccccttcacc ccgatgagtc
     1741 gtcccagtcg gaaaacgacc gcccgctcgt actcgcggag gacgcggacg gaattgccca
     1801 ggatgatcag gccaacgagg aagaggacgg gaaggaggct cagggtcaac gtgttcatcg
     1861 ggtttctcca gcggcgggag agggagagtc cggccgtacc ttgagggaga gccggtcaac
     1921 ggccacgacg cggacggctt gtccggcgtc gacgatcgaa tcgtgcgggg cttcggcttc
     1981 ccagtgggtg ccgtccatga agacgctccc cgtgggagcc agcttcgttg cggccacgcc
     2041 ccggcggccg atcatggcgg cggcgccggt ggccgccggg cggcgctggg cggccagccc
     2101 cttgccgacg aggaagagga agaaggcggc ggtcacgact acggcgggaa gcatcac
//
LOCUS       DASVOD010000707         6989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_619602, whole genome shotgun sequence.
ACCESSION   DASVOD010000707 DASVOD010000000
VERSION     DASVOD010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6989
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619602"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VF720_15200"
     CDS             <1..520
                     /locus_tag="VF720_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX7880760.1"
                     /translation="LARPGVAAQAVGLAQGALELATVYATRRRQFGSTIASFQMIQKL
                     LADMSSRTEAARQLVYAAARALDSGETNTTKLAAQAKLFATDTAMWVTTQAVQIFGGY
                     GYMRDYPIEKYMRDAKITQIYEGTNQVQSMVVARNLVKEAGQLAYLSRFIPTETQRGV
                     SAADLEGAPTTS"
     gene            complement(563..1084)
                     /locus_tag="VF720_15205"
     CDS             complement(563..1084)
                     /locus_tag="VF720_15205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012675364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX7880761.1"
                     /translation="MAIVRPHHDVHPSIPPSAFLAETAVIIGEVTLGEQVSVWYHTVI
                     RGDVHWIRIGDRTNVQDGTVIHVTNGRYPTDIGSDVVIGHMAMIHGARIRERCLIGMS
                     SLLLDDCEIGAESIVAAGSLVPPGMIVPPRSLMMGRPARRVRDVTDEEVESMILAGVR
                     NYLAYQETYRTPG"
     gene            complement(1090..1851)
                     /locus_tag="VF720_15210"
     CDS             complement(1090..1851)
                     /locus_tag="VF720_15210"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX7880762.1"
                     /translation="MSPLQIDRRERHVWVRLDKGKTHSIDPDMVGRLRALFEELAHDD
                     SVGAVLLSGLGDRFFCNGLDVDCLLALNRREMTRFVDDFLGLCRDLYLFPRPLVAAIN
                     GHAMAGGLILALAADYRIVCDENRSLGFTEIRLGLPVPREAVMILSELVGNRTAHRLV
                     LSGEVLLPEAAYRAGLVHEVTSFRHLETVAETVAAERAALPHVAYARTKRYLRSATAE
                     LMAPLAASSRDEFLECWYLPETQAALKRLAEKRRG"
     gene            complement(1884..2564)
                     /locus_tag="VF720_15215"
     CDS             complement(1884..2564)
                     /locus_tag="VF720_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880763.1"
                     /translation="MADILSLWVGALLTLMIFSFLYKDNPFYKFAEHFFVGVSAAYWM
                     CTAFWNTLMPNLFGKIHPPMIGPWFPDLAKAPADPIFWVPLVFGVLLLMRLVPPVDHL
                     SRWAVALIVGWTAGTNMMRYMQSDFVGQIYSTIRPLVVTTNGQFILGDTFNNIVLFIG
                     VLCGLVYFFFSKEHTGTFGGAARVGIWFLMITFGAAFGYTVMARISLLIGRMQFLGRW
                     LSEGFGAG"
     gene            complement(2564..3439)
                     /locus_tag="VF720_15220"
     CDS             complement(2564..3439)
                     /locus_tag="VF720_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880764.1"
                     /translation="MSRRETHAHPLARIDRRWIFLLIAVSVIIPMLFKLKIPTRPSPI
                     VQDIYDRVESLPPGSKVLLSMDFAPGTIPENWPMAAAAVRHTLVKGHKLYMMTLWATG
                     PDIITRAVDEIVKVDFPGAKYGTDYVNLGYKAGNQGVINSIVANFKTLFTGDATGTPT
                     NAIEMTQPINTVGDFDLVIAIGSGFPGVKEWVQFAGDRSNVPIAGGVTAVEAPLLYPY
                     YPRQLLGLMGGLQGAAEYEAALVAGHPEFKAKSEDAISKMGPQTVAHLVILALIVVGN
                     VAYFLGKSAERKGRA"
     gene            complement(3441..4202)
                     /locus_tag="VF720_15225"
     CDS             complement(3441..4202)
                     /locus_tag="VF720_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880765.1"
                     /translation="MTPGLFALLSILVVLAGVGASVAIVPLRRNLPLLITFLVGSVMV
                     ASVFIPHAPFDEADDKFSIFFNIIAAIAFILGGLNLLKIHGEKISRRAHHWQYNLVTV
                     VAFLLMLGAGFWSLAFPGTWKGSVQSPGTFFNWMYQSTFSPLQSTMFSLLAFFVASAS
                     YRAFRAKTKEASLLLGAATIILLGRTPLGHYLTFWLPDPLQMLHIPTLSGWILGVPNL
                     AGQRAILIGIALGIISTSLKIILGIERSYLGAKDS"
     gene            complement(4234..5139)
                     /locus_tag="VF720_15230"
     CDS             complement(4234..5139)
                     /locus_tag="VF720_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6754 domain-containing protein"
                     /protein_id="HEX7880766.1"
                     /translation="MALPFRERIPAITLIGAILLSLPAASAFGAEAEAAWFNPAKINL
                     LVIAIIVSAAILIFTFLAGRGREFYIRKIAAITAVEEAVGRATEMGKTVLFVPGNQDM
                     DNVQTVAGLTILSSVARKTAEYETSLTVPTSRSLVMATGREVVKQSYQAAGRPDLFRD
                     DMVYYVTDEQFGYVAALDGFMLREKPAACFYLGAFFAESLVLAETGNSIGAIQVAGTA
                     QPTQLPFFVAACDYTLIGEELFAASAYLSRDLRMLGSLRGQDVGKALAMIAIVVGCLL
                     MTISSVTGGMSSLADGFKRLFALNF"
     gene            5250..5681
                     /locus_tag="VF720_15235"
     CDS             5250..5681
                     /locus_tag="VF720_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880767.1"
                     /translation="MSGMRPQPEIRLARALRAGALYDWLLAAIILVAHPALFRLFRTP
                     PPVDLFHFRVNSLALVLLGLLYWETARDPLGRRWATRLAALIRFVGGALLILITLAHR
                     PAGAGTFLAFGMLDFAWGALLVALLRAGSKKYPPSQGDDGG"
     gene            complement(5981..>6989)
                     /locus_tag="VF720_15240"
     CDS             complement(5981..>6989)
                     /locus_tag="VF720_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880768.1"
                     /translation="TGIDVRFSRMTRFDGDGGYSFGAHRWVGMGGLEFGRRNDGLLFR
                     LEGSDQIQPFGPNRIEAGAGFLSLIAGQDRQEYLRRSQLSAWFSPVRSDDGEVRLGAW
                     VRHDRPTPAVTDDHLFGGGTPMERPNPLVEDVERRGLTLVGAGNWRQGLVVASGSADV
                     AEPGSDGDDDGYSAQEAAVTIRPIVAGGTLSMTLEGRNTAGSPPVQEEPFLGGDATLR
                     GYDPLEFTGRRRLRMRVEYESGVDILKRAHIPVLETMRLQFIPFADVGTTWGSGKGVN
                     EETPVVLDGEARASFGLGIRKELWIPGVRAIRVDVSRRADGNSDHPVDVWVRLLPYVE
                     W"
BASE COUNT         1262 a         2318 c         2287 g         1122 t
ORIGIN      
        1 cctggcccgc cccggcgtgg ccgcgcaggc cgtgggcctg gcccaggggg cgctcgagct
       61 ggccacggtg tacgccacgc ggcggcgcca gttcggcagc accatcgcga gcttccagat
      121 gatccagaag ctgctggccg acatgtcgtc ccgcaccgaa gccgcccgcc agctggtgta
      181 cgctgcggcg cgcgccctcg actcgggcga gacgaacacc accaagctgg ccgcccaggc
      241 caagctcttc gccaccgaca cggccatgtg ggtcaccacg caggccgtcc agatcttcgg
      301 cggctacggc tacatgcgcg actacccgat cgagaaatac atgcgcgacg ccaagatcac
      361 ccagatctac gaagggacca accaggtcca gtcgatggtg gtggcccgca acctcgtgaa
      421 ggaagccggc cagctggcgt acctctccag gttcatcccc accgaaacgc agcgcggggt
      481 gtcggcggcc gacctggaag gcgcgcctac gacgagctga ggcgcacgtg actcgccgcg
      541 cgcttttcgc gccgggccgg tgctaacccg gagtgcgata cgtctcctgg taggccaggt
      601 aattcctgac gccggccagg atcatcgact ccacctcttc gtcggtcacg tcgcgtacgc
      661 gtcgggcggg gcgccccatc atgaggctcc ggggcggcac gatcatcccg ggcggcacca
      721 gcgatccggc ggccacgatc gattcggcac cgatctcgca atcatcgagc agcaacgacg
      781 acatcccgat caggcagcgc tctcggatcc tggccccgtg gatcatcgcc atgtggccga
      841 tgacgacgtc gctgccgatg tcggtcgggt accggccgtt ggtcacgtgg atcaccgtgc
      901 cgtcctgcac attggtccgg tcgccgatcc ggatccagtg cacatcgccg cggatcacgg
      961 tgtggtacca cacgctcacc tgctccccca gcgtcacctc gccgatgatc accgccgtct
     1021 cggccaggaa cgcggagggt gggatggagg ggtggacgtc gtgatggggc cggacgatcg
     1081 ccacggggct cagccccggc gcttctcagc gagtcgcttg agggcggcct gcgtctcggg
     1141 caggtaccag cactccagga attcgtcgcg cgaggaggcc gccagcggcg ccatcagctc
     1201 ggcggtggcg ctcctcaggt agcgcttcgt gcgggcatag gccacgtgcg ggagcgcggc
     1261 cctctccgcc gccacggttt cggccaccgt ctcaaggtgc cggaaggatg tcacctcgtg
     1321 cacgaggccc gcacggtagg cggcttcggg caggagcacc tcgcccgaaa ggaccagacg
     1381 atgcgcggtg cggttcccca ccagctcgga caggatcatc accgcctcgc gcggcacggg
     1441 gagccccagt cgtatctcgg tgaagcccag cgagcgattc tcgtcgcaca cgatccggta
     1501 gtcggccgcc agcgccagga tcaacccgcc ggccatggcg tgcccgttga tggcagccac
     1561 cagcggccgg gggaacaggt agaggtcgcg acacaggccc aggaagtcgt cgacgaagcg
     1621 cgtcatctcc cgccggttca gggcgaggag gcagtccacg tccaggccgt tgcagaagaa
     1681 tcggtcgccg aggcccgaga gaagcacggc gccgaccgag tcgtcgtggg ccagctcttc
     1741 gaacagggcg cgcagccggc ccaccatgtc ggggtcgatg ctgtgggtct ttcccttgtc
     1801 caggcgtacc cagacatgtc gttcccggcg atcgatctgg agggggctca tgcggggatc
     1861 ataccgccgc gccgacgggg ctgtcagccg gcgccgaaac cctcgctgag ccatcggccg
     1921 agaaactgca tgcggccgat caggagcgag atgcgcgcca tcaccgtata accgaacgcc
     1981 gccccgaacg tgatcatcag gaaccagatc ccgacgcggg cggccccccc gaacgtacct
     2041 gtgtgctcct tcgagaagaa gaagtagacg aggccgcaca ggacgccgat gaacagcacg
     2101 atgttgttga acgtgtcccc gaggatgaat tgcccgttgg tggtcacgac caggggacgg
     2161 atggtggaat agatctgccc gacgaaatcg gactgcatgt accgcatcat gttcgtgccg
     2221 gccgtccacc ccacgataag ggccaccgcc cagcgcgaaa ggtggtcgac gggcggcacg
     2281 agccgcatga gcaggagcac gccgaaaacc agcggcaccc agaagatcgg atcggccggc
     2341 gccttggcaa gatcggggaa ccacggcccg atcatcggcg gatggatctt gccgaacagg
     2401 ttgggcatca gggtgttcca gaacgccgtg cacatccagt aggccgccga aactcccacg
     2461 aagaagtgct cggcgaactt gtagaacggg ttgtccttgt acaggaacga aaagatcatc
     2521 agcgtcagca gtgcgccgac ccaaaggctc aggatgtccg ccatcaggcg cgccccttcc
     2581 gctccgccga cttcccgagg aagtaggcca cgttgcccac cacgatgagc gccaggatca
     2641 ccaggtgcgc caccgtctgg ggtcccatct tgctgatggc gtcctcgctc ttggccttga
     2701 attcagggtg tcccgccacg agggcggcct cgtactcggc ggctccctgg aggccgccca
     2761 tcaggcccag gagctggcgc gggtagtagg ggtagaggag gggcgcctcg acggccgtca
     2821 ccccgccggc gatcggcacg ttcgagcgat cccccgcgaa ctgcacccat tccttcacgc
     2881 cgggaaagcc cgacccgatg gcgatcacca ggtcgaagtc gccgacggtg ttgatgggct
     2941 gcgtcatctc gatggcgttc gtcggcgtgc ccgtggcgtc accggtgaag agagtcttga
     3001 agttggccac gatgctgttg atgaccccct ggttgccggc cttgtatccg aggttcacgt
     3061 agtcggtgcc gtatttcgcg cccgggaagt cgaccttcac gatctcgtca accgcccggg
     3121 tgatgatgtc gggccctgtg gcccacagcg tcatcatgta gagcttgtgc cccttcacca
     3181 gcgtgtggcg gaccgccgcc gccgccatcg gccagttctc ggggatcgtg cccggtgcaa
     3241 agtccatcga cagcagcacc ttcgagcccg gcggcagcga ctcgacccgg tcatagatgt
     3301 cctggacgat gggactcggg cgggtcggaa ttttcagctt gaacagcatc gggatgatca
     3361 ccgacacggc gatcagcagg aaaatccagc ggcggtcgat ccgggccagc ggatgggcgt
     3421 gagtttcgcg tcggctcatg tcaggagtcc tttgcgccca ggtaggagcg ctcgatgccc
     3481 aggatgatct tgagcgacgt ggaaatgatt cccagtgcga taccgatcag gatggcccgc
     3541 tggccggcca ggttcggcac gccgagtatc catcccgaga gagtggggat gtgcagcatc
     3601 tggagcgggt cggggagcca gaatgtcagg tagtgaccga gcggcgtgcg ccccaggagg
     3661 atgatggtgg cggcgccgag cagcagtgag gcctccttgg tcttggcccg gaaggcccgg
     3721 taagaggcgg aggccacgaa gaaggccagc agcgagaaca tcgtcgactg cagcgggctg
     3781 aatgtcgact ggtacatcca gttgaagaag gtgccggggg actgcaccga acccttccag
     3841 gtgcccggga acgccagcga ccagaaaccg gcgccgagca tcaggaggaa cgccacgacg
     3901 gtcaccaggt tgtactgcca gtgatgggcc cgccgcgaga tcttctcgcc gtggatcttg
     3961 aggaggttca atccccccag gatgaaggcg atggcggcga tgatgttgaa gaagatcgag
     4021 aacttgtcgt cggcctcatc gaacggcgcg tgggggatga aaaccgaggc caccatcacc
     4081 gatccgacca ggaaggtgat gagaaggggc aggttccgac ggagcggaac gatcgccacc
     4141 gaagctccca cccccgccag cacgacgagg atgctcaata gtgcgaagag tccgggcgtc
     4201 atcagtctcc ctggctggcg gcctggccgc cgctcagaag ttcagtgcga agagccgctt
     4261 gaatccgtcg gccagcgacg acatgccgcc ggtaacgctc gaaatcgtca tcagcaggca
     4321 ccccaccacg atcgcgatca tcgcgagggc ctttccgacg tcctgcccgc ggaggctgcc
     4381 gagcatgcgc aggtctcggg agaggtaggc gctggccgcg aagagttcct cgccgatcag
     4441 cgtgtagtcg caggcggcca cgaagaatgg cagctgcgtg ggttgggcgg tgccggccac
     4501 ctggatggcg ccgatcgagt tgccggtctc ggccaacacc agcgattcgg cgaagaaggc
     4561 tcccaggtag aaacaggccg ccggtttttc gcgcaacatg aaaccgtcaa gtgcggcgac
     4621 atagccaaac tgctcgtcgg tcacgtagta gaccatgtcg tcccggaaca ggtcggggcg
     4681 gcccgccgcc tggtacgact gcttcacgac ctcccggccg gtggccatga ccagcgagcg
     4741 ggaggtgggc accgtcaacg acgtctcgta ctcggcggtc ttcctcgcca ccgacgagag
     4801 gatagtcagg ccggccaccg tctggacgtt gtccatgtcc tgattgccgg ggacgaagag
     4861 cacggtcttc cccatttcgg tggcccggcc gacggcctct tccaccgccg tgatcgcggc
     4921 gatcttacgg atgtagaact cgcgtccgcg ccccgccagg aaggtgaaga tgaggatggc
     4981 ggccgacacg atgatcgcga tgacgagcag gttgatcttg gcgggattga accaggccgc
     5041 ttcagcctcc gccccgaaag cggacgcggc gggaagggaa agaaggatgg cgcctatcag
     5101 cgtgatcgcc gggatccgtt cgcggaacgg gagcgccatg cacgacacct cggggtcgaa
     5161 tcgccacgtg cggtggacgg tcgtacgagc ggtggacaat aaggcgcggc ctgcgcccga
     5221 tcaacccctg tattcgttac aattggtcca tgtccggcat gcgcccgcaa cccgaaatcc
     5281 ggttggcccg ggccctgcgc gccggcgcac tgtacgattg gctcctcgcc gccatcatcc
     5341 tggtggccca tcccgcccta tttcgcctct tccgcacgcc acctccggtc gatctcttcc
     5401 acttccgggt taactcgctc gcgctggtcc tgctcgggct gctctactgg gaaactgccc
     5461 gcgaccccct cggacgacga tgggcgaccc gactcgcagc cttgatccgt tttgtcggag
     5521 gcgcgctact catcctgatc acgctggcac atcgaccggc gggtgccggc accttcctcg
     5581 ccttcggcat gctcgacttc gcctggggcg cgctcctcgt cgcgctcctg cgggcgggat
     5641 caaaaaaata cccgccatcg cagggggacg atggcgggtg aaccgacggc gtttgccgtc
     5701 ggctgcttcg ctccaatacc aaggcaatac ccgtgcccga tccgcaccgt gggcgtaaat
     5761 tttgtatctc gctgtccgat caccaattac gcgcgtcggc tccgatcccc tgaacatggt
     5821 atcccccggt acctgtaagc ccactcgaca aaacctgcag ggcacctgcg ggtcccgtgt
     5881 cgaatgcgcg ctccagttca ttgggagcaa acgacttgca gctttcgtcc tggccccggg
     5941 taactgtcgg aattccgaca ccgacaatcg agtcgacaga tcaccattcg acatacggaa
     6001 ggaggcggac ccagacatcc acgggatgat cggaattccc gtcggcacga cggctcacgt
     6061 cgacgcggat cgcccgcact ccgggaatcc acaattcctt gcgaatcccg agtccgaacg
     6121 aggcgcgcgc ctcgccatcg aggaccacgg gcgtctcctc gttgacgccc ttccccgaac
     6181 cccacgtcgt tcccacgtcg gcgaacggaa tgaactggag ccgcatcgtc tcgaggaccg
     6241 ggatgtgcgc gcgcttcagg atgtcgactc cggattcgta ctcgacgcgc atccgcagcc
     6301 gccggcgtcc ggtgaattcg agcggatcgt agccgcgcag cgtggcgtcg ccgccgagga
     6361 agggttcttc ctggacaggt ggcgacccgg cggtgttgcg accctcgagc gtcatggaca
     6421 gggtgcctcc cgccacgatc ggccgaatcg tgacggcggc ctcctgtgcc gaatagccgt
     6481 cgtcgtcacc gtccgagcct ggctcggcca cgtcggcgga tcccgacgcg acgaccagtc
     6541 cctgccgcca gttgcccgcc ccgaccaggg tcagaccgcg gcgttccacg tcttcgacca
     6601 gcgggttcgg tcgttccatc ggcgtcccgc ccccgaaaag gtggtcgtcg gtgacggccg
     6661 gcgttggccg gtcgtgccgg acccaggcgc ccagtcgaac ctcaccatcg tccgaccgga
     6721 cgggggagaa ccaggcactc aactggcttc gccggagata ctcctggcga tcctgtcccg
     6781 ctatgaggga aaggaaaccg gcgccggcct cgatgcgatt cgggccgaag ggctggatct
     6841 ggtcggaccc ttccagccgg aagaggagtc catcgttgcg gcgcccgaac tcgagaccac
     6901 ccatgccgac ccatcggtgg gctccgaagc tgtagccacc gtcgccgtcg aagcgggtca
     6961 tgcgcgagaa gcgcacgtcg atcccggtg
//
LOCUS       DASVOD010000708         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_932289, whole genome shotgun sequence.
ACCESSION   DASVOD010000708 DASVOD010000000
VERSION     DASVOD010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932289"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF720_15245"
     CDS             complement(<1..237)
                     /locus_tag="VF720_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX7880769.1"
                     /translation="MTRDDHLALVREYTTNPSLIKHMLAVEAAMRALARRAGEDEDVW
                     GAVGLLHDFDYERWPELTDHTIKGAEILRERGYSE"
     gene            complement(234..>1503)
                     /locus_tag="VF720_15250"
     CDS             complement(234..>1503)
                     /locus_tag="VF720_15250"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880770.1"
                     /translation="LLGIALFLLIARPDRPWSLGRGAMAVLAGLTALGGKETGLALLP
                     LAVADAWLMPAERGRRRWIAPAIALAVTILFLAVRTMVVGWPRYEAAPGLDLLMNPLA
                     GLGFMERLPAALSIAAWYARMLVFPWPLLTLDRPASLPGWGDPAVWLGTPLLVAAITS
                     LALAARRRSWTGLAPAWWLSMLALVGQFLTPIGTYREVRLVYPFLGALAFALAAIVAF
                     ASRRGVSRLILGVAAIPIVAWLVVGLGRARDYASEIDLYAADVRHRPESPIAHLMLGV
                     VYGDAGRAAEADRERREALRLAPDSPQALNEVAAVEIRSGNFARADELLQRALAREPR
                     NHVALMNLGNLRAQQDRLAEAHEILLRAEALSPGYSLTQVNLAIVEALGGRPDEASRR
                     ADRLEKQNATDPNVALIRRLVANSRGKVSP"
BASE COUNT          252 a          505 c          536 g          210 t
ORIGIN      
        1 ttcggagtag ccccgttcgc gcaggatctc ggctcccttg atcgtgtggt cggtcagttc
       61 gggccagcgt tcgtagtcga aatcgtgcag gaggcccacc gccccccaca cgtcttcatc
      121 ctcgccggcc cggcgggcca gcgcccgcat ggctgcctcg acggccagca tgtgcttgat
      181 gaggctcggg ttcgtggtgt actcgcgcac gagcgcgagg tgatcgtcgc gtgtcatggc
      241 gaaaccttac cacgcgaatt cgccaccagt cggcggatca gcgccacgtt cgggtcggtg
      301 gcgttctgct tttccaggcg gtcggcccgc cggcttgcct cgtcggggcg cccccccagg
      361 gcttcgacga tggccaggtt gacctgggtg aggctgtaac cggggctcag cgcctcggcc
      421 cgcaggagta tctcgtgggc ttccgccagc ctgtcctgtt gcgcacggag attcccgagg
      481 ttcatgaggg ccacgtggtt cctcggctcc cgggccaggg cgcgctggag gagttcgtcg
      541 gcccgggcga agttgccgga gcggatctcg acggcggcca cttcgttcaa cgcctgtggc
      601 gagtcaggcg cgaggcggag cgcctcccgg cgttccctgt ccgcttccgc cgcacgaccg
      661 gcatcgccgt acacgacacc cagcatcagg tgagcgatcg gcgattcggg gcgatgacgc
      721 acgtccgccg cgtagaggtc gatctccgag gcgtagtcgc gggcgcgacc gaggcccacg
      781 acgagccagg ccacgatcgg gatcgcggcg acgccgagga tcagccgcga caccccccgc
      841 cgggaagcga aggcaacgat ggccgccagg gcaaacgcca gcgcaccgag gaacgggtaa
      901 acgagccgca cctcgcgata ggtgccgatc ggcgtaagga actgcccgac gagagcaagc
      961 atggacagcc accaggccgg tgcaagtccc gtccaggaac ggcgccgtgc ggccagtgcg
     1021 aggctcgtga tcgccgcgac gagaagcggg gttcccagcc acacggccgg atccccccat
     1081 ccgggaagcg aagccggtcg atcgagcgtg aggagcggcc aggggaagac cagcatgcgg
     1141 gcataccacg ccgcgatgga aagcgcggcg ggtagacgct ccatgaagcc gagaccggcc
     1201 agcggattca tgaggagatc gaggccgggt gccgcttcgt accgcggcca gccgaccacc
     1261 atcgtgcgga ccgcaaggaa gagaatcgtt acggccagcg cgatcgcggg agcgatccag
     1321 cggcgacgtc ccctttcggc gggcatgagc cacgcgtccg ccaccgcgag cggcagcagg
     1381 gcaagtcccg tttcctttcc gccgagggcc gtgaggcctg cgaggacggc catcgctcca
     1441 cgccccaggc tccatggccg atcgggccgc gcaatgagga ggaagagggc gatgccgagg
     1501 agg
//
LOCUS       DASVOD010000709         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_50124, whole genome shotgun sequence.
ACCESSION   DASVOD010000709 DASVOD010000000
VERSION     DASVOD010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50124"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..176
                     /locus_tag="VF720_15255"
     tRNA            101..176
                     /locus_tag="VF720_15255"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:cgc)
     gene            248..512
                     /gene="ffs"
                     /locus_tag="VF720_15260"
     ncRNA           248..512
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF720_15260"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            607..>1802
                     /gene="dnaX"
                     /locus_tag="VF720_15265"
     CDS             607..>1802
                     /gene="dnaX"
                     /locus_tag="VF720_15265"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEX7880771.1"
                     /translation="MAYVVLARKWRPQRFEDVVGQEHITGTLANAISQNRVAHGYLFC
                     GPRGVGKTTTARILAKALNCEKGPTPTPCNQCSACKDVDNGTSFDILEVDGASTRGID
                     DIRELRDNARYTPAGGRTKVYIIDEVHMLTREAFNALLKTLEEPPPHVVFVMATTEAY
                     KVPATILSRCQRFDFARIHPRAVAARLQHICNEEGIAIDEAAVAVLARRSAGGLRDAL
                     SSLDQVLASAGEGEAARINEAMVDRVLGILSTDFYFGLADRLIDGDAAGALRQLDDAW
                     HGGADLAEIAEELARHFRNVMVAALGPASEGALEELSAHEIGLLREQAAKVGPVTGAR
                     LLEVALRALNVSRRSEQVRLHLEMALVELSQVSQALSLGDVAKKLAEMESRLGGAPQV
                     AGPVRSS"
BASE COUNT          302 a          596 c          605 g          299 t
ORIGIN      
        1 gtaaccgtat cgagggttcg aatccctctc tctccgccag ttttacgcag cgggcggcta
       61 tcaggtagac tgtcggccga atttgcccga gtaccttttc ggggatgtag ctcagctggg
      121 agagcgcctc gttcgcaacg aggaggtcga cggttcgatc ccgttcatct ccaccaaaaa
      181 tacgacgggc tcgggtcctg ttggggccgg agcccgtcgg tgttttgaca ggcatgtcga
      241 ataagggtcg tgctaggtgg ggaaccagcg gtgccctgta acctgcaatc cgctatagca
      301 ggacggaatt cccgatcgag ggccttgaaa ccgggttttg ccgcgggaga ggtggtgtcg
      361 aaggccagac ccgtcgcaac cgggcgtcca tgaaccccgc caggaccgga aggtagcaac
      421 ggtaagtggt tccgcaggtg tgccggcggc catctggccg gagccggccg cccgcagcgg
      481 actcggaatc aggttcggaa tcgggtgcac gaccctcttt acttcgcccg gcatgccggg
      541 cgaagcgctc tttcctttgc ttttgccctg tgacatcgtt ttaccggtgt tggaggaaag
      601 ccccgaatgg cctacgtagt ccttgctcgc aaatggcgcc cccagcgctt cgaggacgtg
      661 gtcggccagg aacacatcac cggcaccctg gccaacgcca tttcccagaa ccgggtggcc
      721 cacggctacc tcttctgcgg cccgcgcggc gtcggcaaga ccaccaccgc ccgcatcctc
      781 gccaaggccc tgaactgcga gaaggggccg acgcccaccc cgtgcaacca gtgctccgcc
      841 tgcaaggacg tggacaacgg caccagcttc gacatcctcg aagtggacgg cgccagtacc
      901 cgtggcatcg acgacatccg cgagctgcgc gacaacgccc gctacacccc ggccggcggc
      961 cggaccaagg tgtatatcat cgacgaagtg cacatgctca cccgcgaggc gttcaacgcg
     1021 ctgctcaaga cactggaaga gccgccgccc cacgtggtct tcgtgatggc cacgaccgag
     1081 gcctacaagg tgccggccac gatcctctcg cgctgccagc gcttcgactt cgcccgcatc
     1141 catccgcgcg ccgtggcggc ccgcctccag cacatctgca acgaggaggg gatcgccatt
     1201 gacgaagccg ccgtggccgt cctggcccgc cgctcggccg gtggattgcg ggacgcactt
     1261 tcgtcgctcg accaggtgct ggcctcggcc ggcgaaggag aagccgcccg catcaacgag
     1321 gcgatggtcg accgggtgct cggcatcctt tccaccgatt tctacttcgg cctggccgac
     1381 cgcctcatcg acggcgatgc cgccggcgcg ctccgccagc tcgatgacgc ctggcacggc
     1441 ggcgccgacc tggccgagat cgccgaggaa ctggcccgcc atttccggaa cgtgatggtg
     1501 gcggcgctcg ggcccgcgtc ggaaggtgcg ctcgaggaat tgagcgcgca cgagatcggg
     1561 ctgctccggg aacaggccgc gaaggttggc ccggtgaccg gcgcccggct gctcgaggtg
     1621 gcgctgcgtg cgctgaacgt gtcgcggcgc agtgagcagg tgcggttgca cctggagatg
     1681 gcgctcgtgg agctgagcca ggtgagccag gccctttcgc tgggggatgt ggcgaaaaag
     1741 ctggcggaga tggagagccg cctgggcggc gcgccgcagg tggcgggtcc ggtgcggtca
     1801 tc
//
LOCUS       DASVOD010000710         2381 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_358499, whole genome shotgun sequence.
ACCESSION   DASVOD010000710 DASVOD010000000
VERSION     DASVOD010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358499"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VF720_15270"
     CDS             complement(<1..1007)
                     /locus_tag="VF720_15270"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX7880772.1"
                     /translation="MATTLVDVQKRILPNGLTILVREDHASPVVALNFWVHVGSNNES
                     GDLMGWAHGIEHMLFKGTERRPAGAIAREIKNAGGEVNAATGYETTNYYIVVPREEYK
                     AALEIHADVMRNSVFDPAELENERQVLIEENQMYRDRPSGYGRTWEELFRLGFTTHRY
                     QSPIGGPDRNLRDTPRDTIVGYKERYYVPANIVYVITGDVVAEEAFAAVQAVLGDWPE
                     VPFSPDRSPEEPEQERFRFKEIEGDVSRVYGKIGFHVSSELADDHDALHVLSHILGVG
                     RSSRLFREVREAQQLVHSISVLSVSGVDPGYLVIDFTADTGKALDALVAIFHEVGRLT
                     R"
     gene            complement(1144..>2381)
                     /locus_tag="VF720_15275"
     CDS             complement(1144..>2381)
                     /locus_tag="VF720_15275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880773.1"
                     /translation="TFLTWILMPYLVLSILFNRELGGARDWDLVATLAVPSILLVGLT
                     FAAAPGPAGGDSRRERRTPVTGGASPALAVTLLGASLFHLVGFVLVDTDHARSRRHFL
                     ALFDEGAPVSRFARSYALEEVGRDDIEHDRYDEAIQHFEEAVRVDPANMKAIGTLGSL
                     YNSRGDAARALPHLERAVRLRPDIGLNHYNLGAALSTAGRPGDAALSFNQALEIDETI
                     EPAWVGLANMALALQMVAQADSVASEGLRRFPTNADLLVAAAVAREQLGRPAEAINLF
                     DAALRAAPDNRSALFNLGRLHNSQGQFALTIPILERLTALEPHDAEAWNNLGVARLQL
                     GRLDEAGRAFMSALSAAPDLVPAVGNFAQVLVRQGRREEAMGVLQSFLDRRPDLARPA
                     GIDRMLDAVRRGAGATGAP"
BASE COUNT          369 a          792 c          842 g          378 t
ORIGIN      
        1 tcccgcgtca gacgccccac ctcgtggaaa atggccacca gggcatcgag cgccttcccc
       61 gtgtcggcgg tgaagtcgat gaccaggtaa ccgggatcaa cgcccgacac cgagaggacg
      121 ctgatggagt ggacgagctg ctgcgcctcc cgcacctcgc ggaagaggcg gctcgagcgc
      181 ccgacgccca ggatgtgcga caggacgtgg agtgcgtcgt ggtcgtcggc cagctcgctc
      241 gagacgtgga agccgatctt cccgtacacg cgggaaacat ccccctcgat ttccttgaac
      301 cggaaacgct cctgctccgg ctcctcgggg gaacgatcgg gcgagaacgg cacctccggc
      361 cagtcgccca ggacggcctg gacggctgcg aacgcctcct cggccacgac gtcgccggtg
      421 atcacgtaga cgatgttggc cggaacgtag tagcgctcct tgtaacccac gatggtgtcg
      481 cgcggcgtat cgcgcaggtt ccggtccggc ccgccgatcg gcgactggta gcgatgcgtc
      541 gtgaagccga gccggaagag ctcctcccag gtgcgcccgt agccggaggg gcggtcgcgg
      601 tacatctggt tttcctcgat cagcacctgc cgctcgtttt cgagttcggc cggatcgaag
      661 accgagttcc gcatcacgtc ggcgtgaatt tcgagggcgg ccttgtactc ctccctgggc
      721 acgacaatat agtagttggt ggtttcgtag ccggtcgcgg cgttcacctc gccgcccgcg
      781 ttcttgatct cccgggcgat ggcgccggcc gggcgccgtt ccgttccctt gaagagcatg
      841 tgctcgatgc cgtgggccca ccccatcagg tcgcccgatt cgttgttcga gccgacgtgc
      901 acccagaaat tgagggccac caccggggat gcgtggtctt cccggaccag gatggtcagg
      961 ccattcggga gaatccgttt ctgcacatcg acgagcgtcg tggccaagag gcctccggga
     1021 gcggctcagg gggttcgggt ggctcggggc cggcgcgtcc ggccccgggc caaatccgga
     1081 tgatggtagg aacgccgcgc ggtgcggtca agctaaccgc cgcgaagtgg cggagttggc
     1141 ggctcagggt gcgccggttg ccccggcgcc ccgcctcacg gcatccagca tccggtcgat
     1201 cccggcgggt cgtgccaggt cggggcgccg gtcgaggaac gactggagca cgcccatggc
     1261 ctcttcccgg cgcccctgcc ggaccagcac ctgggcaaag ttgcctacgg ccggtacgag
     1321 gtccggggcg gccgaaaggg cgctcatgaa ggcgcggccg gcctcgtcca gtctgccgag
     1381 ttgcaggcgc gccacgccga gattgttcca ggcttccgcg tcgtgcggct cgagtgccgt
     1441 gagcctttcg agtatcggta tcgtgagcgc gaattgtccc tgggagttgt ggagacgccc
     1501 gaggttgaaa agggccgatc ggttgtccgg cgcggcgcgc agggccgcat cgaacaggtt
     1561 gatcgcctcg gcggggcggc cgagttgctc ccgggccacg gctgccgcca ccaggagatc
     1621 ggcgttggtc gggaaccgcc tgagcccctc ggacgccacc gaatcggcct gggccaccat
     1681 ctggagggcc agtgccatgt tggccagccc cacccaggcc ggctcgatcg tctcgtcgat
     1741 ctcaagcgcc tggttgaacg aaagcgccgc atcgcccggc cggccggcgg tgctgagcgc
     1801 cgcgccgagg ttgtagtggt tgaggccgat gtccggccgg aggcgtaccg cccgttccag
     1861 gtgcggaagc gctcgcgccg cgtcgccccg gctgttgtag aggctgccga gcgtgccgat
     1921 cgccttcatg ttggccggat cgacccggac cgcctcctcg aagtgctgga tggcctcgtc
     1981 gtagcgatcg tgctcgatgt catcgcgacc gacctcctcg agggcgtacg agcgggcgaa
     2041 gcgcgagacg ggtgccccct cgtcgaacag cgccaggaag tggcggcggg accgcgcatg
     2101 atcggtgtcg accagcacga aaccgaccag gtggaagaga gaggcgccga gcagcgtgac
     2161 cgccagcgcc ggcgacgcgc cgccggtgac cggcgtccgc cgttctcgtc gcgaatcccc
     2221 ccctgccggc ccgggtgcgg ccgcgaacgt cagcccgacg aggaggatcg acggcaccgc
     2281 cagcgtggcc accaggtccc agtcgcgggc gccgccgagt tcccggttga agaggatcga
     2341 gagtacgagg tacggcatca ggatccacgt cagaaaggtg a
//
LOCUS       DASVOD010000711         3380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_100275, whole genome shotgun sequence.
ACCESSION   DASVOD010000711 DASVOD010000000
VERSION     DASVOD010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100275"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..2251
                     /locus_tag="VF720_15280"
     CDS             131..2251
                     /locus_tag="VF720_15280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7880774.1"
                     /translation="MTAETDRRPALGALLVAAGMILVAAFAWLSRPSAGAPPFHQYEL
                     EFLYRIPRGEAGFYLRYLALGLPAVLLLGFGVSFLTGRSFWDRFLARLDAVGERFFVA
                     VIMVTAALLVLGVARFILHDQPVTDDERVYLFQADLLSRGHLYLESQPLREFYDNVFI
                     VNNGKWYGKYPPGHPLFLVPGVVAGFPRIVPALLAALNVLLLYRLAGFYFSRRLARAA
                     ALLLLLSPFFLFTGATLLSHTSCLTMLLLFAWAAHRSMAGGNTALVAGAAVGMAFLIR
                     PYTALIIGLPVAAAWFLALRRRGAPLKPWLTALGTASLFAIALLVVNQQLTGNPFRTG
                     YAEVQGDRGRVLGFGEVIPGRLEHTPASGLLNVLQTIVRLNFWSFGWPLGWLFVVAAL
                     LWSRRRDLLPLWGILVVTALGSVLYFSLGVSDTGPVKYYELLPVMVLLTVAGAASLNE
                     RIGRAGRPGLSGLAPALIAALTLVGWGLFGRVQAAELGALTNRIAAPYRQVAQLPTDQ
                     RLLIFASTLQRSPFDSWVFSTPNNVPESEARVLYVRDRGKDNRAIIERMADRAPYVLQ
                     KNEDQRFELTRLSGDEASRRMVEEREREAIAHLRKLEIKEAIDLLAQASKLDPNRAKT
                     YLLLGWACEQGRLAGPAETAYRKALELDKNNPDHYFFLGRFLGRANRLDEALPLLEAA
                     VRANPRQKDYATALGQVRSGVPPP"
     gene            complement(2307..>3380)
                     /locus_tag="VF720_15285"
     CDS             complement(2307..>3380)
                     /locus_tag="VF720_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7880775.1"
                     /translation="KWIQSWRDLPLLLNQWANVVRWEKVTRPFLRTTEFLWQEGHTAH
                     ATAGEAHEETIRMLDVYARFAEEYMAVPVLKGPKTDAEKFAGAVRTYSIEAMMQDRKA
                     LQAGTSHDLGQNFAKAFDVTYQDSEGKREHVWATSWGVSTRLVGALIMCHSDDDGLVL
                     PPKLAPMPVVIVPIWMKDEQKEEMSAAALAIKSELAGVAGVHVDARDQYKPGWKFAEW
                     ELKGVPVRVEVGPRDMAQGQVTLVRRDTKAKETIARGDVGRRVPELLDEIQAALFQRA
                     LEFREANSRSLDDYAEFKKQVEEPGGFFWMPWCGGAACEAKVKEETKATIRCLPFDAK
                     AEAGSCVVCGGASERRVVWAKAY"
BASE COUNT          479 a         1263 c         1025 g          613 t
ORIGIN      
        1 accggcgggt ggtgacactg ggtggcccgg agccggaact ctcacgcgcg cgcgagcgcg
       61 ctactcagct cggacggaac ggcgccgccc cgcccgcacc tcccgacgga aaccgaaata
      121 cgaatcgatg atgaccgccg aaacggatcg acgacccgcc ctcggtgccc tcctcgtggc
      181 agcggggatg atcctggtgg ccgccttcgc ctggctctcc cgcccctcgg ccggcgcgcc
      241 cccgttccac cagtacgaac tcgagttcct ctaccgcatt ccccgtggcg aggccggctt
      301 ctatctccgt tacctggccc tcggcctgcc ggccgtcctt ctcctgggat tcggcgtgtc
      361 gttcctcacc gggcggtcgt tctgggaccg attcctcgcc cgcctcgatg ccgtcggcga
      421 gcgattcttc gtcgcggtga tcatggtcac cgcggcgctg ctggtgttgg gcgtggcccg
      481 gttcatcctg cacgaccagc cggtcaccga cgacgagcgc gtctacctct tccaggccga
      541 tctcctgtcg cgcggacacc tgtatctcga atcgcagccg ctgcgcgagt tctacgacaa
      601 cgtcttcatc gtgaacaacg gcaagtggta cgggaagtat ccgccgggac accccctctt
      661 tctcgtaccc ggcgtggtgg ccggcttccc ccggatcgtc cccgccctgc tcgccgcgct
      721 gaacgtcctg ctcctctaca gactggccgg cttctacttc tcccggcggc tggcccgggc
      781 ggccgcgctt ctcctgctcc tgtcgccctt cttcctcttc acgggggcca cgcttctttc
      841 gcacacgagc tgcctcacca tgctgctcct ctttgcatgg gcggcgcacc gctcgatggc
      901 cggcggcaac accgcgctcg tggccggcgc cgccgtgggg atggccttcc tcatccgccc
      961 ctacacggcg ttgatcatcg gccttccggt ggccgccgcc tggttcctgg cgcttcggcg
     1021 tcgcggcgcg ccgctcaagc cctggctcac ggcgctcggt accgcatcgc ttttcgcgat
     1081 cgcccttctg gtggtgaacc agcagctcac cggcaatcct ttccgaaccg gctacgccga
     1141 agtccaggga gatcgcgggc gcgtactcgg cttcggcgaa gtgatcccgg gacggctcga
     1201 gcacacgccc gcgtccggcc tcctcaacgt gctccagacg atcgtccggc tcaacttctg
     1261 gtcgttcggc tggccgctcg gctggctctt cgtggtggcg gccctgctct ggagtcgtcg
     1321 tcgtgacctc ctgccgttgt ggggcatcct cgtcgtgacg gcgctcggca gcgtcctcta
     1381 tttctcgctc ggggtcagcg acacgggccc ggtcaagtac tacgaactcc ttccggtgat
     1441 ggtcctcctc accgtggcgg gggccgccag cctcaacgag cggatcgggc gggccggtcg
     1501 gcccgggtta tcgggactgg ccccggcgct gattgccgcg ctcacactgg tcggctgggg
     1561 cctcttcggt cgcgtgcagg ccgcggagct cggggcgttg acgaaccgga tcgccgctcc
     1621 ctacaggcag gtggcgcagc tccccacgga ccagcgactc ctcatcttcg cgagcaccct
     1681 gcagcgctcg cccttcgact cgtgggtctt ctccactccc aacaacgttc cggaatccga
     1741 ggcacgcgtg ctttacgtgc gcgaccgtgg caaggacaac cgcgccatca tcgaacgcat
     1801 ggccgaccgg gcgccctacg tgctccagaa aaacgaggac cagcgtttcg agctcactcg
     1861 cctctcaggc gacgaggcct cccgtcggat ggttgaagaa cgggaacgcg aagccatcgc
     1921 ccatcttcgc aagctggaga tcaaggaagc catcgatctc ctcgcgcagg ccagcaagct
     1981 cgacccgaac cgtgccaaga cctacctgct cctgggctgg gcctgtgaac aggggcgcct
     2041 ggcggggccg gccgagacgg cctatcggaa ggcgctcgag ctcgacaaga acaatcccga
     2101 tcactacttc ttcctgggac gattcctcgg ccgggccaac cgcctggacg aggcgctgcc
     2161 gctcctcgaa gcggccgtcc gggccaatcc ccgccagaag gactacgcca cggcgctcgg
     2221 ccaggtccga tcgggcgtgc cgcctccctg agcgggttcg atcggcaagt aagcgggctt
     2281 gatcggatgc ggcgccgact gaagccttaa taggccttgg cccacaccac gcgacgctcc
     2341 gaggccccgc cgcataccac gcacgatccg gcctcggcct tcgcgtcgaa cggcaggcag
     2401 cggatggtgg ccttggtctc ttccttcacc ttcgcctcgc acgcggcgcc gccgcaccac
     2461 ggcatccaga agaagccgcc cggctcctcg acctgcttct tgaactcggc gtagtcgtcc
     2521 agtgaacgcg agttggcctc gcggaactcc agcgcccgct ggaacagcgc ggcctggatt
     2581 tcgtcgagca gttccggcac gcggcgtccc acgtcgcccc gggcgatcgt ctccttggcc
     2641 ttcgtgtcgc gccgcaccag cgtcacctgc ccctgggcca tgtcgcgcgg gccgacctcc
     2701 acgcgcaccg gcacgccctt caactcccac tcggcgaact tccaacccgg cttgtactgg
     2761 tcccgcgcgt cgacatgcac gccggcgacg ccggccagct cggacttgat cgcgagggcg
     2821 gcggccgaca tctcctcttt ctgttcgtcc ttcatccaga tcggcacgat gaccaccggc
     2881 atcggcgcca gcttcggcgg caggacgagc ccgtcatcgt cgctgtgaca catgatgagc
     2941 gcacccacca ggcgcgtgct caccccccag ctggtggccc acacatgctc gcgctttccc
     3001 tcggagtcct gataggtgac gtcgaaggcc ttcgcgaaat tctgcccgag gtcgtgcgac
     3061 gtgccggcct gcagcgcctt ccggtcctgc atcatggctt cgatcgaata ggtccgcacc
     3121 gcaccggcga acttctccgc gtcggtcttc ggacccttca gcaccggcac ggccatgtac
     3181 tcctcggcga accgggcgta cacgtcgagc atccggatcg tttcctcgtg cgcctctccc
     3241 gccgtggcgt gggcggtgtg cccttcctgc cacaggaact cggtcgtgcg caggaagggg
     3301 cgcgtcacct tctcccagcg aacgacattc gcccattgat tgagaagcag cggcaaatcg
     3361 cgccacgact ggatccactt
//
LOCUS       DASVOD010000712         5683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_100313, whole genome shotgun sequence.
ACCESSION   DASVOD010000712 DASVOD010000000
VERSION     DASVOD010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100313"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VF720_15290"
     CDS             <1..1122
                     /locus_tag="VF720_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880776.1"
                     /translation="KDPDLMRNVNAHLKRWFRGVSHSLERGESLRDQDMLLLTQLCML
                     EINLMERRVSRLASRVDPYDGRSISRLMPVLSFYDQDIEHLKSVVSRLATYKPFYERL
                     LTMEHLLSGSEMDKLEKQLQKEVFGQAVGQVVGMVRRNPILDRELAFLTSAVYQVALL
                     RKEAMPKEPAPDLLSILHGILETVQDEPRVHVVIEPELAKSLFATVQDWGFVHLLPDI
                     FVLTYREEWAGNFVLPDGTPSLPDRPGKPPDAPTSVRELIQRMLSNDAFLVGILENSC
                     VTGMPGIVPMIAMQTRSVRVLDKILSSRNLMTGPANKEVPRLILTNPARVPMQSLKSI
                     INVRYVSRVDLDRLAKPTSDVRPEVRSEITAYLRILRTT"
     gene            complement(1134..1901)
                     /locus_tag="VF720_15295"
     CDS             complement(1134..1901)
                     /locus_tag="VF720_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880777.1"
                     /translation="MIIGGGCYGCYHARQLLRGAVKGALEFQELVIVDRDPACAARKE
                     FAGQAGVRFVTADWVDFVDDYLDRALTGTADVMVPTPIGPHLMYEWLRRRVERRSVLH
                     AAAFERLPATPYAERSPTGTGYLSFATWTCPVTCVEPAICPVTRGPKEWEMDEAVFAF
                     ARDEAGEGRPFDEVTVFRCRPLAFGIAAWPTAHALRVRDRFDRLLAMWAPEGLADGVV
                     PRACRILVATTSSCHGVADLLELHPRHTGPAGERNPA"
     gene            complement(1925..2644)
                     /locus_tag="VF720_15300"
     CDS             complement(1925..2644)
                     /locus_tag="VF720_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880778.1"
                     /translation="MSQGGKKLVVPAVLFILVIIGLSALDARLKGPVASSRNLEGKRT
                     DKEEALAERAFLNAQLALAASDSINIVLDARTNHLWIVMKGVKLRECKLSDVQFDANI
                     RQLVSGGNEALWLERPFILAERRGSLPDPWQPVAEGAVDTLKGGVAVKNLPMNGALVF
                     DRELVLHIRTPPTAADSLSYKGLAGMTKRWNDRLAEAGDAWAELTAGPASMDVYLHIS
                     REDAAAVLRALSVGGGMALRI"
     gene            complement(2695..3534)
                     /locus_tag="VF720_15305"
     CDS             complement(2695..3534)
                     /locus_tag="VF720_15305"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7880779.1"
                     /translation="MNSQAGYTIEGIANSARPRTASSGRSRWPVIALGTLALLVGAIN
                     AYLWLAGSGRAGAAVAQVEKDAATWRESWATQQAPPPAKGKKSSKGKNAKSSKTPGML
                     AVGPLPSGDAPIEEINSQAQDLEGQLARYQPDGVFIVIDTANNKFLMRDGADTLLNTD
                     CSTGSYSSLTAADGRKWFFHTPRGQFKIQNKKQKPVWVKPDWAFVETNEPIPSARAEE
                     RYEPYVMGDYAMGFGNGYYIHGTLYGRLIGQSVTHGCVRLLDAPLEQVYNTAPIGTPI
                     FIY"
     gene            complement(3577..4704)
                     /locus_tag="VF720_15310"
     CDS             complement(3577..4704)
                     /locus_tag="VF720_15310"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEX7880780.1"
                     /translation="MPESEAPPPKRRRRRWPWVAGVLILVTGAVTAILRADKPPMLLL
                     ETARKSISNLQAVEVSEWAPELLKAAQDSLRFASQEVDRENRRFFVIRDYDAARSQAL
                     AADRIARETIPIALARRDSVKQEAADNLVEAAESIKSAREMLEYLPMAKTSRSQVVQL
                     ELTRQQARRSLDEGLYRKSIDLTESVLSGAENIKASVSRQMDNYHDPIWVKWARDTIA
                     GSRASGGYAILIRKFEHRCDVYHAGVLKTSFPVELGLRWMGQKMRAGDHVTPEGRYHV
                     TNKKNGSRYYRALEINYPNAEDEVRFRLAKQSGAIPKRASIGGLIEIHGGGGKGKDWT
                     QGCVALTNANIDKLFAMVGAGTPVTIVGSYDPDGTGALAAK"
     gene            complement(4834..5448)
                     /locus_tag="VF720_15315"
     CDS             complement(4834..5448)
                     /locus_tag="VF720_15315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880781.1"
                     /translation="MTFNRRNTLLATALVATTLLITACAKVPQEELTAAQAALDKARA
                     AEAEKYAPDTWRAASDSLAAANAEIQAQASKFALFRKYDRTNALIASANMSAAKASQD
                     AVVNKEAAKNAANEALATATAAVDSAKTVLATAPVGKDNRADVEMMKADLTGAETNLA
                     QVSTAISSEDYFGARAQAEAITAKANQIINDVMMAKEKMMGGRH"
BASE COUNT          889 a         1918 c         1884 g          992 t
ORIGIN      
        1 aaggaccccg acctgatgcg gaacgtcaac gcccacctca agcggtggtt ccgcggcgta
       61 tcgcattcgc tcgagcgggg cgaatcgctc cgcgaccagg acatgctcct cctcacgcag
      121 ctctgcatgc tcgagatcaa cctgatggag cgacgcgtct cgcggctggc cagccgcgtc
      181 gatccctacg acggccggtc gatcagccgc ctcatgccgg tcctgtcgtt ctacgaccag
      241 gacatcgagc acctgaagag tgtggtgagc cgcctcgcca cctacaagcc tttctacgag
      301 cggctcctca ccatggaaca cctcctttcc gggagtgaga tggacaagct cgagaagcag
      361 ctccagaagg aggtgttcgg gcaggcggtc ggccaggtag tcggaatggt tcgcaggaac
      421 ccgatcctcg atcgcgaact ggccttcctg acttcggccg tctaccaggt ggcgctcctg
      481 cgcaaggaag cgatgccgaa ggaaccggcg cccgacctcc tctccatcct gcacggcatc
      541 ctcgaaacgg tgcaggacga gccgcgggtc cacgtggtca tcgagcccga gctggctaaa
      601 agcctttttg ccaccgtcca ggactggggc ttcgtgcacc tgctgcccga catcttcgtc
      661 ctcacctatc gcgaggagtg ggccggcaac ttcgttctgc ccgatggcac gcccagcctc
      721 cccgaccgcc ccggcaagcc ccccgacgcc ccgacgtcgg tccgcgagct gatccagcgc
      781 atgctatcca acgacgcctt cctcgtggga atcctcgaga actcgtgcgt caccggcatg
      841 cccggcatcg tgccgatgat cgccatgcag acgcgcagcg tccgcgtgct cgacaagatc
      901 ctctcgagcc gcaacctcat gacgggaccg gccaacaagg aagttccgcg cctcatcctg
      961 accaacccgg cgcgcgtgcc gatgcagagc ctcaagtcga ttatcaacgt ccgctacgtc
     1021 tcgcgcgtcg acctcgaccg gctggccaag ccgacgtcgg atgtccggcc ggaagtgcgg
     1081 agcgagatca ccgcctacct gcggatcctg aggaccacct aggccgggac ggctcaggcc
     1141 gggttgcgtt ctccggctgg accggtgtgg cgcggatgca gctccagaag gtccgccacg
     1201 ccgtgacagg agctggtggt tgctaccagg atccggcagg cacgcggcac gactccgtcg
     1261 gcgaggccct ccggggccca catcgcgagc aatcgatcga accgatcccg cacgcgcaag
     1321 gcgtgggccg tcggccacgc ggcaatcccg aaggccaatg gacggcagcg gaacaccgtc
     1381 acctcgtcga aggggcgccc ctcacccgcc tcgtcccggg caaaggcaaa gaccgcctcg
     1441 tccatttccc actccttggg tccccgcgtt accgggcaga tggccggctc cacgcaggtg
     1501 accgggcaag tccatgtggc gaaggagagg tagcccgtgc cggtgggtga ccgttcggcg
     1561 tatggcgtgg ccgggagccg ctcgaatgcc gcggcatgca acaccgaccg gcgctcgacc
     1621 cggcgcctga gccactcgta catcaggtgc ggcccgatcg gcgtgggcac catgacgtcg
     1681 gccgtgccgg tgagcgcgcg gtcgagataa tcgtcgacga agtcgaccca gtcggcggtg
     1741 acgaagcgga cgccggcctg gccggcgaat tccttccggg ccgcacaggc aggatcccgg
     1801 tcgacaatga ccaactcctg gaattcgagc gcgcccttga cggcgccccg caggagctgg
     1861 cgggcgtgat agcaaccgta gcagccgccg ccgatgatca cggcgtcgtg gatgatggcc
     1921 ggcgtcagat ccgcagggcc atcccgccgc cgaccgacag cgcccgcagg acggcggcgg
     1981 catcttcacg cgaaatatgc aggtacacat ccatgctggc ggggcccgcg gtcagctcgg
     2041 cccaggcatc gcccgcctcc gccaggcggt cgttccaccg cttcgtcatt cccgcaagac
     2101 ccttgtacga gagtgaatcc gcggcagtcg gcggcgtgcg gatgtggagc acgagttcac
     2161 gatcgaagac gagcgcgccg ttcatcggca ggtttttcac ggccacgcca cccttcagcg
     2221 tgtcgaccgc tccctcggcc accggctgcc acggatcggg caagctcccg cgtcgctcgg
     2281 ccaggatgaa gggccgttcc agccagagag cttcgttgcc gccggagacg agctgacgga
     2341 tgttggcgtc gaactgcacg tcggagagct tgcactcgcg cagcttgacc cccttcatca
     2401 cgatccagag gtggttcgtg cgggcgtcga ggacgatgtt gatggagtcg ctggcggcca
     2461 gcgccagctg ggcgttgaga aaggcccgct cggcaagcgc ctcttccttg tcggtgcgct
     2521 tcccctcgag gttgcgcgag ctggccaccg ggcccttgag gcgggcgtcg agggccgaga
     2581 ggccaatgat gaccaggatg aaaaggacgg ccggcacgac cagcttcttt ccgccctggg
     2641 acatctcccg gccggcccgc cgggcgcgac gactctcccc cgcgtacgcc gtgctcaata
     2701 gatgaagatc ggcgtcccga tcggggccgt gttgtagacc tgctccagcg gagcgtcgag
     2761 cagtcggacg cagccgtggg tgacgctctg gccgatcagg cggccgtaga gcgtgccatg
     2821 gatgtagtaa ccgttgccga agcccatggc gtagtcgccc atgacgtacg gctcatagcg
     2881 ctcctcggcc cgggcgctcg ggatcggctc gttcgtctcg acgaacgccc agtccggctt
     2941 gacccacacg ggcttctgct tcttgttctg gatcttgaac tggccgcgcg gcgtgtggaa
     3001 gaaccatttg cgcccgtcgg ccgcggtcag gctcgagtac gagccggtgg agcagtcggt
     3061 gttgaggagc gtgtcggcgc cgtcacgcat caggaacttg ttgttggccg tgtcgatcac
     3121 gatgaacaca ccgtcgggct ggtacctggc gagctgcccc tcgagatcct gggcctgtga
     3181 attgatctcc tcgatcggcg cgtcgccgct cggcagcggc cccacggcca gcattcccgg
     3241 cgtcttggag gacttggcgt tcttcccctt ggacgacttc tttcccttgg ccggcggcgg
     3301 cgcctgctgg gtggcccagc tctcgcgcca ggtggccgcg tccttctcga cctgcgcgac
     3361 ggcggcgccg gcccggccgg agccggccag ccagagatag gcgttgatgg cgccgacgag
     3421 aagtgccagg gtgccgaggg cgatcacggg ccagcgagac cggccgctcg acgcagtccg
     3481 cgggcgcgcc gaattggcaa tcccctcgat cgtgtagccg gcctggctgt tcatgtcggc
     3541 gatccccttc cctcttggtc ctgcgatgtg ccgtcgctac tttgcggcaa gcgccccggt
     3601 gccgtcggga tcatacgacc cgacgatcgt taccggcgtg ccggcgccca ccatggcgaa
     3661 gagcttgtcg atgttggcgt tggtcagggc cacgcacccc tgcgtccaat ccttcccctt
     3721 gccgccgcca ccgtggattt cgatgagacc tccgatggag gcgcgcttgg ggatggcgcc
     3781 gctctgcttg gccaggcgga agcgaacttc atcttccgca ttcggataat tgatttccag
     3841 ggcgcgatag tagcgcgagc cgttcttctt gttcgtcacg tgatagcgcc cttccggcgt
     3901 cacgtggtct cccgcgcgca tcttctgccc catccaccgc agccccagct ccaccgggaa
     3961 cgacgtcttg agcacccccg cgtggtacac gtcgcagcga tgctcgaact tgcggatgag
     4021 gatggcgtag ccgcccgaag cgcgggaacc ggcgatcgtg tcccgggccc acttcaccca
     4081 gatcgggtcg tggtaattgt ccatctggcg gctgacgctg gccttgatgt tctcggcgcc
     4141 cgagaggacc gactcggtca ggtcgatgga tttgcggtag agcccttcgt ccaggctccg
     4201 cctggcctgc tggcgcgtga gctcgagctg aaccacctgc gagcggctcg ttttggccat
     4261 cggcagatac tcgagcatct ccctggcgct cttgatcgac tcggcggcct cgaccaggtt
     4321 gtcggcggct tcctgcttga ccgaatcccg ccgggccagg gcgatcggaa tcgtttccct
     4381 ggcgatccgg tcggcggcca gcgcctgcga gcgcgcggcg tcgtagtcgc ggatgacgaa
     4441 gaagcggcgg ttctcccggt cgacttcctg ggatgcgaac cggagcgagt cctgggccgc
     4501 tttcagcagt tccggcgccc actcggacac ctcgaccgct tgaagattcg aaatcgattt
     4561 ccgggccgtt tcgaggagga gcatcggggg cttgtcggcc cgaaggatgg cagtaacagc
     4621 tcccgtgacc aggatgagca ccccggccac ccagggccag cgccgccggc ggcgcttcgg
     4681 cggcggcgcc tcggactccg gcacccagag gtacgcatgc ggaagccggc ccctctcacg
     4741 ggagagaggc cggctccgat tggatctccg ctccgaagag cggttgaaaa ctcgatggat
     4801 cagcgtgtca cctcgtgacg cgcgtgcggc tggttagtgc cgcccgccca tcatcttctc
     4861 cttggccatc atgacgtcgt tgatgatctg gttggccttg gcggtgatgg cctcggcctg
     4921 ggcgcgggcg ccgaaatagt cttcgctgga aatggcggtc gacacctgcg cgaggttcgt
     4981 ctcggcgccg gtcagatcgg ccttcatcat ctcgacgtcc gcgcgattgt ccttgccgac
     5041 cggcgcggtg gccaggacgg tcttggcgct gtcaacggcg gcggtggcgg tggccagcgc
     5101 ttcgttggcg gcgttcttgg cggcttcctt gttcacgacg gcgtcctgcg aggccttggc
     5161 agccgacatg ttggcggacg cgatcagggc gttggtgcgg tcatacttgc ggaagagggc
     5221 gaacttgctg gcctgggcct ggatctcggc gttggcggcg gcgagcgagt cgctcgcggc
     5281 gcgccaggta tcgggcgcgt acttctcggc ctcggcggcg cgggccttgt cgagggcggc
     5341 ctgggccgcg gtcagctctt cctgcggcac cttggcgcat gccgtgatga gcagcgtggt
     5401 ggccacgagc gccgtggcga gcagcgtatt ccggcgattg aacgtcatgt gatcccctct
     5461 ccttgcgtga cggacaccgc ccggaccgga aactccggcg gggcggggca gcgatttgcc
     5521 agcttcccgg cgcggggcag ggccccggcg ggacgtcacc gtaagatcac gattcatgcc
     5581 agtcatccgc ccatcctaag acattaccgg acaacgatca acggaatatg atacacggag
     5641 cggcccgtgt ccggtcgaac ccaccgtgtc ggtttggaca cac
//
LOCUS       DASVOD010000713         1512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_619801, whole genome shotgun sequence.
ACCESSION   DASVOD010000713 DASVOD010000000
VERSION     DASVOD010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619801"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VF720_15320"
     CDS             complement(<1..746)
                     /locus_tag="VF720_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7880782.1"
                     /translation="MNQVEPDVPFPLACTGIPGLDDVLHGGLTPDRLYLVEGVPGSGK
                     TTLAIQFLMEGARLGESVLYVTLSETEAELRAVAASHGWDLKGIHIHELAPPEESLLS
                     DEQYTMFHPSEVELSETTQLILAEVQRLKPTRVVFDSLSEVRLLAATPLRYRRQILAL
                     KRYFAGRNCTVILLDDLTAVERDLQVQSIAHAVVLLEQLNPEYGAERRRLRVVKYRGM
                     SFRGGFHDYKIEHGGLVVFPRLVAAEHRREP"
     gene            complement(801..1283)
                     /locus_tag="VF720_15325"
     CDS             complement(801..1283)
                     /locus_tag="VF720_15325"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7880783.1"
                     /translation="MPDTLDIRPLDGPDRERWITECARMLAASEPWITLRRTEATAHG
                     GISDSGQEAWVAIIGDEVVGFMLLVLHGALRGYLRTICLAPGFRGRGLGTRMIRFAEE
                     RVFRDHPNMFLCVSSFNPDARRLYERLGYHFVGELTDFIVRGHSELLYRKTRGPLMPD
                     "
     gene            complement(1298..>1512)
                     /locus_tag="VF720_15330"
     CDS             complement(1298..>1512)
                     /locus_tag="VF720_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880784.1"
                     /translation="LVVMTETIPGFSEDFGHFQARIPGVMYLLGVSDSTEGLRGMPHD
                     DAYAASDRSIRVGARVMAALVLDTLR"
BASE COUNT          267 a          480 c          528 g          237 t
ORIGIN      
        1 ggctcgcggc ggtgctccgc cgccacgagt cgcggaaaga cgacgagccc gccgtgctcg
       61 atcttgtagt cgtggaaccc accccggaac gacatgccgc gatacttgac cacacggagg
      121 cggcgtcgct cggcgccgta ctcgggattc aactgctcga ggaggacgac ggcgtgggcg
      181 atgctctgca cctggaggtc gcgctccacc gcggtcaggt cgtcgagcag gatgacggtg
      241 cagttgcggc cggcaaagta gcgcttgagt gccaggatct gccggcgata gcggagtggc
      301 gtggcggcca ggaggcgtac ctcggagagg gagtcgaaca ccacccgggt gggcttgagt
      361 cgttgcacct cggccaggat gagctgggtg gtttccgaga gttccacctc cgagggatgg
      421 aacatcgtgt actgctcgtc cgagagaagg ctctcctcgg gcggcgccag ttcgtggatg
      481 tggatcccct tgaggtccca gccgtgcgag gcggccacgg cgcgcagctc cgcctcggtt
      541 tccgaaaggg tgacgtagag caccgactcg ccgagcctgg ccccttccat gaggaactga
      601 atcgccaggg tggtcttccc ggagcccggc accccttcca ccaggtagag acgatcgggc
      661 gtgaggccgc cgtgcaggac gtcgtccagt cccggaattc cggtgcacgc aagcgggaag
      721 ggcacatccg gctctacttg attcatggga cgtctccagg gacaaccggg ggagttccgt
      781 gcggccgatc ggggcgcggc tcagtccggc atcagcgggc cgcgtgtctt acggtaaagc
      841 aattccgagt ggccgcgcac gataaagtcg gtgagctcac cgacaaagtg gtagccaagc
      901 cgctcgtaca ggcgccgtgc atccggattg aacgacgaaa cgcacaggaa catgttcggg
      961 tgatcccgga agacccgttc ctcggcaaac cggatcatcc gtgtgccgag gccgcgtccc
     1021 cggaacccgg gcgccaggca gatcgtgcgc aggtagcccc gaagagcacc gtgcagcacg
     1081 agcagcatga acccgacgac ctcgtccccg attatcgcaa cccacgcctc ctggccacta
     1141 tcggagattc ccccatgggc ggtcgcttcc gtccggcgga gggtgatcca gggctcgctg
     1201 gcggccagca tccgggcgca ttcggtgatc cagcgctcgc gatcggggcc gtccagcggg
     1261 cgaatgtcga gcgtgtcggg cacggtgcgc tcctgagtca gcggagggtg tcgagcacga
     1321 gcgcggccat cacccgcgcg ccgacgcgga tcgaacggtc gctggcggca taggcgtcgt
     1381 catgtggcat cccgcgcagg ccctcggtag agtccgaaac gccgagcagg tacatcactc
     1441 ccgggatgcg ggcctggaag tggccgaagt cctcgctgaa gccggggatc gtctcggtca
     1501 tgaccacgag at
//
LOCUS       DASVOD010000714         8589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_410745, whole genome shotgun sequence.
ACCESSION   DASVOD010000714 DASVOD010000000
VERSION     DASVOD010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410745"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VF720_15335"
     CDS             <1..603
                     /locus_tag="VF720_15335"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX7880785.1"
                     /translation="SVFANQGLRPEPLFVTSVIDKDGVVLEENRPRTREAMDPGPLFV
                     LTDMLESVMNHGTGAKARTLGLTVPVAGKSGTTNDYSDAWFMGYTPDVVTGVWIGFDE
                     RKPIGARMSGSVAALPAWVEIMKAATAGRPIHEFSNPSGVVYRNICPVTGLLAREGCP
                     DPKREVFMAGEAPEAECHVHSGAIVDPAAGRNAKDGQDRD"
     gene            complement(614..1969)
                     /locus_tag="VF720_15340"
     CDS             complement(614..1969)
                     /locus_tag="VF720_15340"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX7880786.1"
                     /translation="MSEEPGARPNLDLSPRRLIARKRDGEPLSAAEIESFVRSFLEGG
                     VADYQMSAFLMAVFFRGMTGDETAALTRVMVDSGTRLDLASVPGIKVDKHSTGGVGDK
                     VSIPLAPLVAACGVPVPMISGRGLGHTGGTLDKLESIPGFRTRLPADEFTRVLAEVGY
                     VMGGQSADLAPADRRMYALRDVTGTVESIPLIVSSILSKKVAEGAGALVMDVKFGRGA
                     FMPDVAQAARLGRELARVGRLLGVEVRVFLTDMDRPLGRKIGNALEIAESIELLTGGG
                     PPDLREITIAFGSAMLVLGGKAADDVEGRRRIEGAITDRSGLERFRRLIEAQGGDASV
                     LDDPSRLPRSPIVETVRAPEGGFVLDIDPRTLGEAVIDLGGGRRRAEDAVDPAVGFDL
                     AVARGDAVRRGQPLATIHASDRAGLAAAESAVHRAFRIGPERPAVRPLIAFSGPGDPP
                     A"
     gene            complement(1969..2424)
                     /locus_tag="VF720_15345"
     CDS             complement(1969..2424)
                     /locus_tag="VF720_15345"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014347929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX7880787.1"
                     /translation="MNPGSHLPDVELLARVEALLDRAYGRYSSIRVAAVVMLEDGTLH
                     DGVNVENSSLGLTVCAERNALFGAVADGAAVGTHAPGPRPVRIVFTSNSDDVTVPCGA
                     CRQVIRELAPHAEIVFGRNGTIRKRWASIDELLPFAFDGDWDSRRPGSA"
     gene            complement(2424..3605)
                     /locus_tag="VF720_15350"
     CDS             complement(2424..3605)
                     /locus_tag="VF720_15350"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEX7880788.1"
                     /translation="MKPRAIVIVLDGVGAGPAPDTADYGDTGSDTLVNLARAVGGLAL
                     PNLASLGLGNMRAIQGIPAASNPTGSWGRLREVNPGKDSTSGHWELACVHLDFPFPTY
                     PKGFPDDVITSFEAAIGRRSIGNVVASGTEIIQRLGDEHVATGYPIIYTSADSVFQIA
                     AHEEIVSLDLLYEWCRAARGILVPPHAVGRVIARPFTGASPAYARTGNRRDFSFEPTG
                     RTMLDTIKEAGQPVVAIGKIVDLYAGRGQTEQFLTHSNAEGMSAIGDALDRVDSGLIM
                     ANLVDFDMLWGHRNDTTGFKAGLEVFDAWLGTVLPRLGAGDLLMLTADHGNDPTTDST
                     DHSRELVPMLATGGAVRPGVDVGTRASFSDLGATVLDHLGLPAPEHGHSFLPEIARTR
                     T"
     gene            complement(3602..4858)
                     /gene="alr"
                     /locus_tag="VF720_15355"
     CDS             complement(3602..4858)
                     /gene="alr"
                     /locus_tag="VF720_15355"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX7880789.1"
                     /translation="MDFPTWVEIDLDRLDRNLRSMAALAGSDVGILFVVKADGYGLGA
                     VEVGRSALAAGATMLGVATLHEGIELRQSGLEAPIVILSPNLEAEFSEVLAWRLTPTL
                     STLEQADHLAIAARGAGRRAAAHVEVDTGMGRTGFDLDEAHAAIRRIAAMTEIELEGI
                     CTHFPDSDGPDRSFTDTQIDRFRELLARLETGGIVPRWRHAANTAGVLAYPDARFNMI
                     RPGLGVLGLLPSPHVTAACSLEPVVSLHARLVQVRKVPPGRHISYGTTFITSRPSRIG
                     VTTIGYGHGLPRRLSNLGAMAVAGKRVPIVGRVTMDLTMVDLTDHPAARVGDDVVVFG
                     EPDGAVPAAESVTLDEVAAWAGTITWEILCQIDKRVVRKYLRGGRVDRVMTLVGERLE
                     AGEGAGVVYSGRQRSVRTTTSGGWRK"
     gene            4959..5756
                     /gene="mazG"
                     /locus_tag="VF720_15360"
     CDS             4959..5756
                     /gene="mazG"
                     /locus_tag="VF720_15360"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEX7880790.1"
                     /translation="MIDESPRALARLIDVCRRLMDEDGCPWDREQTLESITPYLQEEL
                     HEILDAIAAADAPGVAEEMGDLFYLLVFAAALARKQGMALELSQVLNGASEKLIRRHP
                     HVFGQTAVDGTAGAVAQWEEIKKAESKSSTTLGKRPAGLAALTTAYRIQEKAAAVRFE
                     WVDVRGALAKLREEIDELEATLDATGSDADHRRQDEIGDVLYSVVNLARYVRVDPELA
                     LRGTIAKFMNRFQFIESELATRGRTPATSDLEEMDALWNEAKRRGIR"
     gene            complement(5753..6343)
                     /locus_tag="VF720_15365"
     CDS             complement(5753..6343)
                     /locus_tag="VF720_15365"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7880791.1"
                     /translation="MDEMTNGGAEKAELGRRIREARQLRGMTLKEMDESAGLSATHIS
                     EIERGKTSPTIGALIRIAAALGREASFFVEPQVLPDIARTSRSERFQQPFGSARIEVQ
                     TPGVPGGRLKALTLHLEPSVPLRLAVTEGSIAGYIVTGGLHIDSDGSTEQLGPGDSVH
                     VHLDVPVTLTATGAGAEVFVVTTADLSPAVAASRPA"
     gene            complement(6353..6919)
                     /locus_tag="VF720_15370"
     CDS             complement(6353..6919)
                     /locus_tag="VF720_15370"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX7880792.1"
                     /translation="MSAATGVALGRRIRQIRIEKGLTLKQIEARVGISATHVSEVERG
                     RTSPTVGALARIAEALGVRPSHLIDFPIGRHRTVTREGKRALLTMPDRRIDTEILVPS
                     DPAAEISVFVVTLAASFADGVPGEARTGDKLFHILEGAADLVIGEKVMPLEAGDTIHI
                     RSNHAHRLVNRFQTPCRILWALWPRMTI"
     gene            complement(6916..7812)
                     /gene="speB"
                     /locus_tag="VF720_15375"
     CDS             complement(6916..7812)
                     /gene="speB"
                     /locus_tag="VF720_15375"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX7880793.1"
                     /translation="MVSCPDLPFEPPNNFGGLPAADSSFESSPLVILPVPLEQTTSYR
                     GGTKDGPAALIHASRFMELADWEVGDVPASRGIHTLPALEPRASGPAESLDDIETAVR
                     WLLASGKRVGALGGEHSLTYPIIKAYREVWPDLSVLQFDAHGDLRAAYHGSSFSHASV
                     MRRIVDAGVPTVGLGIRSVCEEEIELIRDRQLARFSGRDVARRRIPMETMLETLTDNV
                     YVTLDLDGFDPSEAPGVGTPEPGGFSWFDFLEVMEALGKRRVIGFDVVELMPIPGQAV
                     SEFLAAKAAYVLASALLRGLDR"
     gene            8122..>8589
                     /locus_tag="VF720_15380"
     CDS             8122..>8589
                     /locus_tag="VF720_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880794.1"
                     /translation="MDLDRPISRKLDFVLAVGAVLLLWGPGLTLPGGARPAFTSRAQA
                     AFSPAPAPSNPCSMDDPQVLPVAAGTGIDQYAAYQVTVTGYSSNIHETDDSPGLTAAN
                     TEARSGVIALSQDLLREFTPGAPFSFHDRVEIPGLGQFRVEDTMHPRWTRRADV"
BASE COUNT         1485 a         2781 c         2947 g         1376 t
ORIGIN      
        1 tccgtcttcg ccaaccaggg actgcgtccg gagcctctct tcgtcacttc cgtgatcgac
       61 aaggatggcg tggtgctgga ggagaaccgg ccacggacac gggaagcgat ggatccgggg
      121 ccgttgttcg tgctgacgga catgctggaa agcgtcatga accacggtac cggtgccaag
      181 gccagaacgc tcggtctcac cgtgccggtc gccggaaagt cgggcacgac gaacgattac
      241 tcggatgcct ggttcatggg atacacaccc gatgtcgtga cgggtgtctg gatcggcttc
      301 gacgagcgga agccgatcgg tgcgcgcatg agcggatcgg tggcggcgct gccggcgtgg
      361 gtggaaatca tgaaggccgc caccgcgggc cggccgatcc atgaattctc caatccgagc
      421 ggggttgtct accggaacat atgcccggtg acgggactcc tggcacgcga aggttgcccg
      481 gatccgaagc gcgaagtctt catggccgga gaagccccgg aagcggaatg ccacgtgcat
      541 tccggcgcca tcgtcgatcc ggcggccggc cggaatgcaa aggacggcca ggaccgcgac
      601 tgaccccgga acgttacgcc ggtgggtccc cgggcccgga gaaagcgatg agcggacgaa
      661 cggcgggccg ctccggaccg atgcggaacg cgcgatggac ggccgattcc gccgcggcga
      721 ggccggcccg gtcggaagcg tggatcgtgg ccaatggctg cccgcggcgc accgcgtcgc
      781 cccttgccac cgccaagtcg aagcccacgg ccggatcaac ggcgtcttcg gctcgacgac
      841 gtcccccccc gaggtcgatg acggcttcgc cgagtgtgcg cggatcgatg tcgagcacga
      901 aaccgccctc gggcgcgcgg accgtctcga caatcggtga gcgaggcagc cgggagggat
      961 cgtcgaggac ggaagcgtcg ccgccctggg cctcgatgag gcggcgaaag cgttcgaggc
     1021 cgcttcggtc agtgatggct ccctcgatcc ggcgacgccc ctcgacgtcg tccgccgctt
     1081 ttccgcccag gacgagcatg gccgagccga atgcgatcgt gatttcccgg aggtccggcg
     1141 gcccaccgcc ggttaggagt tcgatcgact cggcgatctc gagcgcgttg ccgatttttc
     1201 ttccgagcgg tcgatccatg tcggtgagga agacacgcac ttcgacgccc aggagtcggc
     1261 ccacgcgcgc cagctcgcga ccgagtcggg ccgcctgggc cacgtcgggc atgaaggccc
     1321 cccggccgaa cttgacgtcc atcacgaggg cgccggcccc ttcggccact ttcttcgaca
     1381 ggatgctgga aacgatcagg gggatcgact cgacggtacc ggtgacatcg cggagcgcgt
     1441 acatccgccg atccgccggg gccagatccg ccgattgccc acccatgaca tatcccacct
     1501 cggcgaggac gcgggtgaac tcatcggccg gaaggcgcgt gcgaaagccg ggaatggact
     1561 cgagcttgtc gagcgtgccg ccggtgtgcc ccagtccccg gccggaaatc atcgggaccg
     1621 gcacaccgca ggcggcaacc agcggggcaa ggggaatgga gaccttgtct cccacgccgc
     1681 cggtggagtg cttgtcgacc ttgatacccg gcacgctcgc gaggtcgagc cgggtgccgc
     1741 tgtcgaccat gacgcgcgtg agggcggcgg tctcgtcccc ggtcatcccg cggaagaaaa
     1801 cagccatgag gaatgccgac atctggtagt cggcgacacc gccctcgagg aaggagcgca
     1861 cgaacgattc gatctcggcg gccgaaaggg gttcgccgtc ccgttttcgg gcgatcagcc
     1921 ggcgtggcga gaggtccagg ttgggccgcg cgcccggctc ctcgctcatc aggccgatcc
     1981 cggtcgacgc gaatcccagt cgccgtcgaa ggcaaacggg aggagttcgt cgatcgatgc
     2041 ccagcgtttc cggatggtgc cattacggcc gaacacgatt tccgcgtgcg gcgccagctc
     2101 ccggatgacc tggcgacagg cgccgcaggg aaccgtgacg tcgtcgctgt tggacgtgaa
     2161 gacgatccgg accgggcgtg gccccggagc atgcgtaccc actgcggcgc catccgcgac
     2221 ggccccgaac agcgcgttcc gctcggcgca aacggtcagt ccgagcgaac tgttctcgac
     2281 attgacgcca tcatggaggg tgccgtcctc gagcatcacg acggctgcca cccggatgct
     2341 ggagtaacgg ccataggccc ggtcgagaag ggcctcgacg cgggcgagga gttcgacgtc
     2401 ggggagatgg gagccgggat tcatcacgtg cgtgttctcg cgatctccgg gaggaaggag
     2461 tggccgtgct ctggcgccgg aaggccgaga tggtcgagga ccgtggcgcc gagatcggaa
     2521 aaggaggccc gggtgccgac atccacgccg ggacggacgg cgccgcccgt ggcgagcatc
     2581 ggcacgagct cccgcgaatg atcggtggag tcggtcgtcg gatcgttgcc gtgatcggcc
     2641 gtgagcatca gtagatcgcc cgctccgagc cgggggagca ccgtgccgag ccaggcgtca
     2701 aacacttcga ggcccgcctt gaacccggtg gtgtcgttgc ggtgccccca gagcatgtcg
     2761 aagtcgacca ggttggccat gatcagcccg ctgtcgacac ggtcgagcgc gtcaccgatg
     2821 gcggacatcc cttcggcgtt gctgtgagtc aggaattgct ccgtctggcc gcgcccggcg
     2881 tacaggtcaa cgatcttgcc gatggccacg accggctggc cggcttcctt gatggtgtcc
     2941 agcatcgtcc ggccggtggg ctcgaaggag aaatcgcgcc ggttgccggt gcgggcatag
     3001 gccggtgatg cgccggtgaa cggacgcgcg atgacgcgcc cgaccgcatg cggcggcacc
     3061 aggattccac gcgcggcgcg acaccactcg tagagtagat cgagtgacac gatctcctcg
     3121 tgggccgcga tctggaagac gctgtccgcc gacgtgtaga tgatggggta cccggtggcc
     3181 acgtgctcat ccccgagacg ctggatgatc tcggttccgg aagccacgac gttgccgatc
     3241 gatcgccggc ctatggccgc ctcgaaggag gtgatgacgt cgtcgggaaa tcccttcgga
     3301 taggtcggga agggaaagtc gagatggacg caggcgagtt cccaatggcc cgaggtggaa
     3361 tccttcccgg gatttacctc acggaggcgt ccccaggatc ccgtgggatt gctcgcggcc
     3421 ggtatgccct ggattgccct catgttcccc aggccgaggg atgcgaggtt cggaagcgcg
     3481 agaccgccga cggcccgggc caggttgacg agcgtgtcgc ttcccgtatc gccgtagtcg
     3541 gccgtgtcgg gcgccgggcc ggcgccgacg ccgtcgagca cgatgacgat ggcacggggc
     3601 ttcactttct ccaacctccg ctggtagtgg tgcgcaccga gcgctgccgt cccgagtaaa
     3661 cgactccggc cccttcgccc gcttcaagcc gttcgccgac cagggtcatg acgcgatcga
     3721 cacgaccgcc acgaaggtac ttccgcacga cacgcttgtc gatctggcag aggatttccc
     3781 acgtgatcgt tcccgcccat gcggcaactt catcgagggt gaccgattcg gcagccggca
     3841 cggcgccatc cggctcaccg aagacgacga cgtcatcgcc cacccgcgcg gccggatgat
     3901 ccgtcaggtc gaccatggtg agatccatcg tgacgcggcc gacgatcggc acccgcttcc
     3961 ccgcaacggc catcgccccg aggttggaga gacggcgcgg caggccgtga ccgtagccga
     4021 tcgtggtaac gccgatgcgg gaagggcggc tggtgatgaa tgtggttccg tacgagatgt
     4081 ggcgccccgg aggtaccttc cggacctgga caaggcgggc gtgcagcgag acgaccggct
     4141 cgaggctgca ggcggcggtc acgtgcgggg acggaagtag cccgaggacg ccgaggccgg
     4201 gccggatcat gttgaagcgg gcatcgggat aagcgaggac gcccgccgtg ttggccgcat
     4261 gacgccagcg cgggacgatg ccgcccgtct cgaggcgcgc cagcaactcg cggaatcgat
     4321 cgatctgggt gtcggtgaag cttcgatccg ggccgtcgct gtccggaaag tgggtgcaga
     4381 tcccttccag ttcgatttcc gtcattgcgg cgattctgcg gatggcggca tgcgcctcgt
     4441 cgagatcgaa gcccgtcctt cccatgccgg tgtccacctc gacgtgggcc gcagcgcgac
     4501 gacctgcccc tcgcgcggcg atcgccaggt ggtcggcctg ctcgagcgtg gaaagggtcg
     4561 gtgtgagccg ccacgcgagg acttccgaaa actccgcttc gagattgggg gagaggatga
     4621 cgatcggggc ctcgaggccg gactgcctga gctcgatccc ctcgtggagc gtggccacgc
     4681 cgagcatcgt ggccccggcc gcgagcgccg accggccgac ttcgaccgcg ccgagcccat
     4741 agccatccgc cttgacgacg aagaggatcc cgacatcgct gccggcgagg gccgccatgg
     4801 atcgaaggtt tctgtcgagg cggtcgagat cgatctcgac ccaggttgga aagtccatga
     4861 aactccgctt cgggggccgc tgaacgcgtc gagtatcaca cccgtcccgt gggccatcaa
     4921 ggccgccgcg tctcgcgagc gcgtggtatc gtgccccgat gatcgacgaa tcaccacgcg
     4981 ctctcgcgcg actgatcgac gtgtgccgcc ggctgatgga cgaggacggc tgcccctggg
     5041 accgtgaaca gacgctggag tccatcactc cctatctcca ggaggaactg cacgaaattc
     5101 tcgacgccat agccgcggcc gacgctccgg gcgtggccga ggagatgggc gacctcttct
     5161 acctgcttgt cttcgcggcc gcgctcgcgc ggaagcaggg aatggcgctc gagctgtcgc
     5221 aggtcctgaa cggggcatcg gagaagctga tccgccgtca tccccacgtc ttcggccaaa
     5281 cggcggtgga cggcacggcc ggggcggtgg cccagtggga ggagatcaag aaggccgaat
     5341 cgaagtcatc gaccaccctt ggaaagagac ccgccgggct ggccgccctg acgaccgcgt
     5401 accgcatcca ggagaaagcg gcggccgttc gattcgagtg ggtggatgtc cggggcgccc
     5461 tggccaagct gcgcgaggag atcgacgagc tcgaggccac gctcgacgcg accgggagcg
     5521 acgccgatca caggcgccag gacgagatcg gcgacgtgct ttattcggtg gtgaacctcg
     5581 cgcgctacgt gcgggtggac ccggaactgg cgctccgggg aacgatagcc aagttcatga
     5641 accgtttcca gttcatcgag agtgagctcg ccacccgtgg ccgcaccccc gccacgagcg
     5701 acctcgagga aatggacgcg ctctggaacg aggccaagcg ccgcggcatc cgctaggccg
     5761 gtcgggaagc ggccacggcc ggcgataaat cagccgtcgt gacgacaaag acttcggccc
     5821 ccgccccggt ggccgtgagg gtgactggaa cgtccaggtg gacgtggacc gaatcgccgg
     5881 gaccgagctg ttcggtgctc ccatcgctgt cgatgtgcaa tccgccggtg acgatgtagc
     5941 cggcgatcga gccctcggtg acggcaaggc ggagtggaac ggacggctcc aggtgcagcg
     6001 tgagcgcctt cagccgaccg cccgggactc ccggagtctg gacttcgatc cgcgctgatc
     6061 cgaacggctg ctggaaccgt tccgacctcg aggtgcgcgc aatgtcgggg agcacctggg
     6121 gctccacgaa gaacgatgcc tcccgtccaa gggcggcggc gattcgaatg agggcaccga
     6181 tcgtcggtga tgtctttccc cgttcgatct cggaaatatg ggtggcggag agcccggcgg
     6241 actcgtccat ttccttgagc gtcattcccc gcagctgtcg cgcctcgcga atacgtcgcc
     6301 ccagctcggc tttctcggct cccccgttgg tcatctcgtc catggaattc tcctaaatgg
     6361 tcattcgcgg ccagagcgcc cagaggatcc gacagggggt ctggaatcga ttgaccaggc
     6421 gatgggcgtg gttgctccgg atatggatcg tatcccccgc ttccagcggc atcacctttt
     6481 caccgatcac gagatccgcc gctccctcca ggatgtggaa gagcttgtct ccggtacggg
     6541 cctcaccggg aaccccgtcg gcgaaactgg ctgccagggt cactacgaag acactgatct
     6601 ccgcggccgg atcggacggg accaggattt ccgtgtcgat ccgacggtcg ggcatggtga
     6661 gcagggcgcg cttcccctcg cgggtaacgg tacggtggcg tccgatcgga aagtcgatga
     6721 ggtgcgacgg ccggactccg agcgcctcgg cgatacgcgc cagcgccccg acggtcggcg
     6781 acgtgcgccc gcgctccacc tccgacacgt gcgtggccga gatgcctacc ctggcctcga
     6841 tctgcttcag ggtgagcccc ttttcgatcc gtatctggcg aatccggcgc cccagtgcga
     6901 ccccggtagc ggcactcatc gatcgagccc gcgaagcaga gccgacgcca ggacgtaagc
     6961 cgccttggcg gcaaggaact cgctgaccgc ctggccggga atgggcatca gctcgaccac
     7021 gtcgaatccg atgacgcgcc tcttgccgag tgcctccatc acctcgagga aatcgaacca
     7081 ggagaagccg ccgggttcag gcgtgcccac cccgggtgct tcggatggat cgaagccgtc
     7141 gaggtcgagg gtgacataga cgttgtccgt gagtgtctcg agcattgttt ccatcgggat
     7201 ccggcggcgc gccacgtccc ggccggaaaa ccgggccagc tgtcgatcgc ggatcagctc
     7261 gatttcctcc tcgcaaacgc tccggattcc gagtccgacg gtcgggacac cggcatccac
     7321 gatccggcgc atgacgctgg cgtggctgaa ggaggatccg tgataggccg cccgaaggtc
     7381 cccatgtgca tcgaactgga ggaccgagag atcgggccag acttcccggt acgccttgat
     7441 tatcgggtag gtgaggctgt gctccccacc aagggcaccg acccgcttgc ccgaggccag
     7501 gagccaccgc acggcggtct cgatgtcgtc caggctctcg gcggggccgg aggcgcgtgg
     7561 ttccaaggcc ggcagggtgt gtatcccccg gctcgcgggg acgtctccaa cctcccaatc
     7621 ggcaagctcc atgaagcgtg atgcatggat gagcgcggcc ggaccgtcct tcgtgccgcc
     7681 cctgtaagag gtggtctgct cgagcgggac gggcagaatc accagggggg acgactcgaa
     7741 cgacgagtcg gctgcaggga gtccgccgaa gttgttgggg ggctcgaagg gaagatccgg
     7801 gcaggaaacc atccggtcga ctccttttct ttggaatggg gcaagtcacc ggaaaccggc
     7861 gtgctgcatg agagatagtg gaaatttccg cccgagaatg accgtggaag ccggaaaagt
     7921 caattccaac taattgtaga gccaacgttt acaagtttac cggcataatt gcgggataaa
     7981 ctgccagaaa tgggagggtc cggggcgtgt ccctgccgaa ccatcgggtg ggctcccaag
     8041 ggttgacact cctccccgga agtccctagg ctccttgggt cgccaaccgg gcggcgcttg
     8101 gtaaaccatc aggatcgacg catggatctg gatcgaccga tcagccgaaa gctcgatttc
     8161 gtccttgccg tcggggctgt ccttctcctc tggggccccg ggctgacgct tccgggtggt
     8221 gctcgcccgg cgttcacgtc gcgggcccag gcggccttca gcccggcgcc ggcgccgtcc
     8281 aatccctgca gcatggacga cccgcaggtc cttccggttg ccgccggcac cgggatcgac
     8341 cagtacgcgg cctaccaggt gaccgttacg ggttacagca gcaacatcca cgaaaccgac
     8401 gactcccccg gcctgacggc cgcgaacacc gaggcccgct cgggggttat cgctctttcg
     8461 caggaccttc tccgggagtt cacgccgggg gccccgttct ccttccacga ccgcgtggag
     8521 atcccgggcc tggggcagtt ccgggtggag gacacgatgc acccgcgctg gacccgccgc
     8581 gccgacgtc
//
LOCUS       DASVOD010000715        10636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_516685, whole genome shotgun sequence.
ACCESSION   DASVOD010000715 DASVOD010000000
VERSION     DASVOD010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10636
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516685"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4577
                     /locus_tag="VF720_15385"
     CDS             <1..4577
                     /locus_tag="VF720_15385"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880795.1"
                     /translation="GNLAGANLTFTGAASNTFSGTGPTTDIRTLTINKGTSNANVLEI
                     TTSNFTVQGGNTDAASMAYLTLTNGTLKISGNFTLAGRTFTAAGFTIGTTAGFWLNNP
                     NYTVSGQNGSSTVNWLRVSQGTLNIGTASGNSMQFSNNATVIVEGGAINATGRFCVSA
                     AANNLNYVQTGGTITVCTVGNASATLASFDLGQGTGLDPVITGGTVICRLANTSATIR
                     DYRNEQGPTGGGVTGGTVQFGDAGSGAAKAFQANGIFPDVVISNTSGNHTLTLRAPSF
                     FFNNMRNVTINTGNTLNLGVGTNNLLFISGSSTVINNGTIVSNSAGNRFIYFTPGANG
                     GYTGSGTSTGAMTSLESQAGSVTMSQVNNLVVRRVIIFTGNFLNSGKITLGDGSATLN
                     VIQIGNTTSPTAAGTFDAPLTFNLGTGGQQASYLRTGAARSTGGEINPGRTLVNFTQD
                     DTAAFPPHPLTLSGGDVTVTSALNLTNGRIVTGASKVIANGTVARTAGHVDGNLQKPV
                     AVGAAVARTFEIGAANGYTPVSVTFATVTGAGALVARSTTGDHANIATSPVNPAKSVN
                     RYYTLTNLGTVFTTADETATFLPGDVDGGANTANFVTGKYDSPNWSPTTTGTRTATTT
                     QATGITSFSDFQIGEAQWSITATAGAGGTIAPTGTSYVANGGNLSYTITPSACFVIAD
                     VVVDGVSQGPIASYDFLNVTANHTISATFTAALAYTIVASAGAGGSISPSGNVSVPCG
                     GDQAFTITPDGCHTIADVLVDGVSVGAVANYNFNAVGANHTISASFAVRVYSIVASAG
                     TGGNISPSGNVYVNCGDDQSFTITTDACYTIRDVLVDGVSVGAVSNYSFVGVTSDHTI
                     AAYFQIIPWTLTSSAGPGGSITPTVVVDCGSSYTFTITPDPCYAVADVLVDGVSVGAV
                     TSYEFTNVQDDHTISATFALTTYTITATAGPGGSISPSGNVNVNCGDDQGFTITPDPC
                     YEVVDVIVDGVSQGSLIFHQFNDVQANHTISATFALSTYTITATAGPGGSIVPSGAVP
                     VTCGDDQTFTITPDACHTILDVQVDGSSVGPVANYTFNDVTSDHIIDATFALSQYTIT
                     ATAAPGGSITPSGANLVDCGDDLTFLIEPDLCWAITDVVVDGSSVGAVTSYTFSDVMA
                     NHTIDAYFALTNYTITATAGPNGSIRPSGNVTVGCGEDQKFTIDPAVGFELCELTVDG
                     ESVPVTGSYTFSNVGANHTIHAEFCDIQCPSVAVVAPNGGETLIIGASYPIQWTATDN
                     VSVTCVDILVSYTGLGGPWTSIATCIPNSSPYNWTVVGPASNNAAIKVVAHDAAGNAC
                     DDISDREFRIIDFVVPVLLSQFAANPISDGVELRWKLTDAFEFARVNIERGDLATGPF
                     EALAAPITIEGGQSVLVDRTVEVGQTYFYRLAGITREGQVVTFEPLQATAGRPVTEFA
                     LAPIANNPARDNVRIAFSVPKDSHVRLSVVDVQGRPVATLIDDTKAAGSYQATWSGDS
                     DRNGRAPSGIYFILMEAPGTKLVQRVALVH"
     gene            4761..5087
                     /locus_tag="VF720_15390"
     CDS             4761..5087
                     /locus_tag="VF720_15390"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEX7880796.1"
                     /translation="MSIKSFTLRIDPLLRRQLDELAEASDTPLNRLINEALRDFVGKR
                     SLALEGELQSRIERLRAYRRHDPNHERAIEAFAKAEGTVPDPVEGRATAKSGPIRKKI
                     VRLLNG"
     gene            5080..5688
                     /locus_tag="VF720_15395"
     CDS             5080..5688
                     /locus_tag="VF720_15395"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX7880797.1"
                     /translation="MADWDEDSPTLQANLVGVLREIRDRVGRRTALSVEDARGWHSAI
                     MQGLKVPNPAHVAHFRGEPGLKSLGVRVGRRRGTPPERVATDLQSFEVTLQAAVSELD
                     RRIPHGVLPNDDGIAAVIDLAAWAHAKWVRIHPFANGNGRTARLWANALLMRYGLPPF
                     VRLRPRPDHGYGNAGAAAMQGKWEPTAAAFHAMLGEFLESGA"
     gene            complement(5814..6296)
                     /locus_tag="VF720_15400"
     CDS             complement(5814..6296)
                     /locus_tag="VF720_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015047441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7880798.1"
                     /translation="MAPAKSPVPEGYRTVTPILTFEECSAALDWYVKGLGAEIISKSP
                     GPDGRIMHSAFKIGDSMLMAHDTMMDGKGPKGFGGSPVALWIYVADCDALFNKAIAAG
                     ATQIRPVEDQFWGDRCGTLKDPNGLAWTIATRKEDLTGAELGQRAEVFFKQMAEMMKK
                     "
     gene            complement(6420..6812)
                     /locus_tag="VF720_15405"
     CDS             complement(6420..6812)
                     /locus_tag="VF720_15405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX7880799.1"
                     /translation="MSDLQQEISDLVATLERAWNAADGAAFARPFAEDADFVNVRADH
                     HRTREAIARGHQGIFDTIYKGSVVHYQLASARLLAPGVALAHINARLEVPAGPMAGTL
                     ASLASLVLTGSPGKWQVAAFHNTLVATR"
     gene            6911..8131
                     /locus_tag="VF720_15410"
     CDS             6911..8131
                     /locus_tag="VF720_15410"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX7880800.1"
                     /translation="MSRIDVTPCSTLEDLSAALQPIMHYFGGGFGEADLARWSKIIDI
                     PRMHAAREDGQVIGGAGAFTFEMSVPGGTVPSAGVTVVGVLPTHRRRGVLTAMMRAQL
                     DDVHRRGDPVAWLWASEETIYRQFGYGMASLSGEVEIPKSAIAFERNIERRGVTRIVS
                     DEEALEPFASIYERVRREQPGMFSRTADWWKLRRLADTERGRAGGAGMLNRVLLTIDG
                     RPEAYALYRVHQSFEGAATNGFLNVIEAVGATDQATREIWRYLLDLDWVARVKAAQLP
                     VDHPLVFLMARPRHMRMRLSDALWVRLVDVPKALAARRLTPGEAVVIDVRDSFCPWNA
                     GRYRIDGRSVERTEAAPDLSVEVNALGSVYLGGFTFGQLARAGAIEVLRPGGVMRADA
                     LFPRERAPWCPEIF"
     gene            complement(8260..>10636)
                     /locus_tag="VF720_15415"
     CDS             complement(8260..>10636)
                     /locus_tag="VF720_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880801.1"
                     /translation="LCPASLILLMLAFPASAAWFPDANPVGSLPGFKQLWAMIPGASD
                     DLYLAWDGSGLRLQRFLGSGEVAPGWPIEGLLISEDGADSHPAALGLDTEGGVIVTWL
                     DHRHPGGQNFSDIYAKHILPDGTLAPDWPVGGLPICRYPTQEYALSVLRDDDGGFFIG
                     WHDTRPLGGYPSGDAYLTRILRNGTLDPAWPVNGSSIGQIDTYEGPVLAPDGMRGLFV
                     ARPLREGQIRVHRIGINGTPLPGWPAEGISTAATVGIRPKIVPDGTGGCFVAWMDGRF
                     FLANRWDIRAVRLRNDGTLAAGWLQGGKIVGFTSGEQYDPDLLPDGEGGAYFAWSSQA
                     CTNCGYWPGRATRLDESGQPVPGWTINGTPLQADLSGHSSLCRALPDGKGGLIVASVN
                     NFVHAQHFDPVGTYAANWPPEGKFIYVSGRSPDGRTVYAPDGRGGFYAAWRAIQTTIL
                     LQRVTTDGRVCGTENRPPTQTPPSSGSEITVTVGEPFELSWRFQDPNGLRDDLVVETL
                     SPVPGFLEVTPSLPATGPEVVLTVAGTPGVADAGRYTLTFRARDRCDAAVTGTATLIV
                     NRAPECGSATAIASKPNADGFRNVSLAGIVDPDGDAVDVEVTGIRQDEDPLSPSSGRS
                     CPDARITAGQVSVRDERNGNGDGRVYHLSFTATDGHGGSCQGSASVCIPHDRGANSRC
                     VDGGALFVSTTCDASQNPGLPNRNGAGGGLRLGSAGNLRLSLDGIDSGPASFELCDVT
                     GRAVARWQYSLSAGATQLDLSVGALPMGLYFLRMHAPGGSTHVWRYLHTTR"
BASE COUNT         1913 a         3673 c         3333 g         1717 t
ORIGIN      
        1 atggcaacct ggcaggcgcg aacctcacgt tcaccggcgc cgccagcaac accttcagcg
       61 gcaccggccc cacgaccgac atccgcacgc tcaccatcaa caaggggacg tcgaacgcca
      121 acgtcctcga gatcaccacc agcaacttca ccgtgcaggg cggcaacacc gacgccgcgt
      181 cgatggccta cctcacgctg accaacggca cgctgaagat ttcgggcaac ttcacgctgg
      241 ccggccgcac gttcacggca gcgggcttca ccatcggcac gacggccggc ttctggctga
      301 acaatccgaa ctacacggtt tcgggccaga acgggtcgtc cacggtcaac tggcttcggg
      361 tcagccaggg gacgctgaac atcggcaccg ccagcggaaa ctcgatgcag ttcagcaaca
      421 atgccacggt gatcgtggaa ggcggcgcca tcaacgccac cggccgtttc tgcgtgagcg
      481 cggcggccaa caacctgaac tacgtgcaga ccggcggcac catcaccgtc tgcaccgtcg
      541 gcaacgcgtc ggcgacgctg gccagcttcg acctgggcca gggcacgggc ctcgacccgg
      601 tcatcaccgg cggcacggtc atctgccgcc tggccaacac atcggccacc atccgcgact
      661 atcgcaacga acagggaccc accggtggcg gcgtcaccgg cggaaccgtg cagttcggcg
      721 atgccggctc gggcgcggcc aaggccttcc aggccaacgg catcttcccg gacgtggtga
      781 tctcgaacac ctccggcaac cacacgctca cgctgcgcgc cccctcgttc ttcttcaaca
      841 acatgcgcaa cgtgaccatc aacacgggca acacgctcaa cctgggcgtg ggcaccaaca
      901 acctgctgtt catcagcggc agctccacgg tcatcaataa cggaacgatc gtctcgaaca
      961 gcgccggcaa ccgcttcatc tacttcacgc cgggcgccaa cggcgggtac accggctccg
     1021 gcacgagcac gggcgccatg accagcctcg aatcgcaggc cggttcggtc acgatgagcc
     1081 aggtgaacaa cctggtggtt cgccgcgtga tcatcttcac cggcaacttc ctcaactccg
     1141 gcaagatcac gctcggtgac ggctcggcca cgctgaacgt catccagatc ggcaacacca
     1201 cgtcgcccac ggcggcgggt acgttcgatg ccccgctcac gttcaacctc ggtacgggcg
     1261 ggcagcaggc gtcctacctc cgcaccggcg ccgcgcggtc gacgggcggg gagatcaatc
     1321 ccggccggac gctggtgaac ttcacccagg acgacacggc ggcgttcccg ccgcacccgc
     1381 tcacgctgag tggcggcgac gtcaccgtga ccagtgccct caacctgacc aacggccgca
     1441 tcgtgaccgg tgcgagcaag gtcatcgcga acggcaccgt ggcgcgcacc gccgggcatg
     1501 tggacggcaa tctccagaag cccgtcgccg tcggcgcggc cgtggcgcgc accttcgaga
     1561 tcggtgcggc gaacggctac acgccggtca gcgtcacctt cgccaccgtg accggcgccg
     1621 gggccctcgt ggcccgaagc accaccggag accatgcgaa catcgccacg tcgccggtga
     1681 acccggcgaa gagcgtcaat cgctactaca cgctgaccaa cctcggcacg gtattcacca
     1741 ccgccgatga aaccgccacc ttcctgccgg gcgacgtgga cggcggcgcc aacaccgcca
     1801 acttcgtcac cggcaagtac gactccccga actggagccc cacgaccacc ggcacccgga
     1861 cggccaccac gacccaggcc accggcatca cgtcgttcag cgacttccag atcggcgagg
     1921 cgcagtggag catcacggcc acggcgggcg cgggcggcac gattgccccc acgggaactt
     1981 cgtacgtggc caacggcggc aacctctcgt acaccatcac gccgagcgcc tgcttcgtca
     2041 tcgccgacgt ggtcgtcgac ggcgtctcgc agggcccgat cgccagctac gacttcctca
     2101 acgtcacggc caaccacacg atctcggcca cgttcacggc ggccctggcg tacacgatcg
     2161 tggcgtcggc cggcgcgggc ggctcgatca gcccgagcgg caacgtgtcg gtgccgtgcg
     2221 gcggcgacca ggcgttcacc atcacgccgg atggttgcca cacgatcgcg gacgtcctgg
     2281 tggacggcgt gtcggtcggc gcggtggcca attacaattt caatgccgtg ggtgccaacc
     2341 acaccatctc ggcttcattc gccgtgagag tctactcgat cgtcgcgtcg gccggcacgg
     2401 gtggcaacat cagcccgagc ggcaacgtct acgtgaactg cggtgatgac cagtccttca
     2461 cgatcacgac cgacgcctgc tatacgattc gggacgtcct ggtggacggc gtgtcggtcg
     2521 gtgcggtgtc caactattcg tttgtcggcg tgacgtcgga ccacaccatc gccgcctatt
     2581 tccagatcat cccgtggacc ctcacgtcct cggccggtcc gggcggatcg atcacgccga
     2641 ccgtcgtcgt ggattgcggc tccagctaca ccttcaccat cacacccgac ccctgctacg
     2701 cggtggccga cgtcctggtg gacggcgtct cggtgggtgc cgtgaccagc tacgagttca
     2761 cgaacgtgca ggacgaccac accatcagcg ccacgttcgc cctcaccacc tacaccatca
     2821 cggccacggc cggtccgggc ggttccatca gtccgagcgg caacgtcaac gtgaactgcg
     2881 gcgacgacca gggcttcacc atcacgccgg atccctgcta cgaagtcgtc gacgtcatcg
     2941 tggacggcgt gagtcagggg tccctgatct tccaccagtt caacgacgtg caggccaacc
     3001 acaccatcag cgccaccttt gccctctcca cgtacaccat cacggccacg gccggtccgg
     3061 gaggctccat tgtcccgagc ggcgcggtcc ccgtgacgtg cggcgacgac cagacgttca
     3121 ccatcacgcc ggatgcctgc cacacgatcc tggatgtgca ggtggatggc tcatcggtcg
     3181 gcccggtggc caactacacg ttcaacgacg tgacgtccga ccacatcatc gacgccacgt
     3241 tcgcactgag ccagtacacc atcacggcca cggccgcccc gggcggttcc atcaccccga
     3301 gcggcgccaa cctggtcgat tgcggcgacg acctcacgtt cctgatcgag cccgacctgt
     3361 gctgggcgat caccgacgtg gtggtcgacg gttcgtcggt gggcgcggtg acgagctaca
     3421 cgttcagcga cgtgatggcc aaccacacca tcgacgccta cttcgccctg acgaactaca
     3481 ccatcaccgc caccgccggg ccgaacggtt ccatcagacc gagcggcaac gtgacggtgg
     3541 gctgcggcga ggaccagaag ttcacgatcg accccgccgt cggtttcgag ctctgcgagc
     3601 tgacggtgga cggagagtcg gtccccgtga cgggcagcta cacgttcagc aacgtgggtg
     3661 ccaaccacac gatccacgcc gagttctgcg acatccagtg tccgtcggtc gcggtcgtcg
     3721 cacccaacgg cggtgagacg ctgatcatcg gcgcctcgta cccgatccag tggacggcca
     3781 ccgacaacgt gagcgtcacc tgcgttgaca tcctcgtgtc ctacaccggc ctcggtgggc
     3841 cctggacgtc gatcgccacc tgcatcccga actcgtcgcc gtacaactgg accgtggtcg
     3901 gtccggccag caacaacgcg gccatcaagg tcgtggctca cgatgccgcc ggcaacgcct
     3961 gcgacgacat cagcgaccgg gaattccgca tcatcgactt cgtggtgccg gtcctcctgt
     4021 cgcagttcgc cgccaacccg atttcggatg gcgtggagct gcgctggaag ctgacggacg
     4081 ccttcgagtt cgcccgcgtc aacatcgagc gcggcgacct ggccacgggt ccgttcgagg
     4141 cgctcgctgc cccgatcacg atcgagggcg gccagtcggt tctggtggac cgcacggtcg
     4201 aagtcggcca gacctacttc taccggctgg cgggcatcac ccgtgaaggc caggtcgtca
     4261 cgttcgagcc tctccaggcc acggcgggac gcccggtcac cgagttcgcc ctcgcgccga
     4321 tagccaacaa cccggcccgc gacaacgtgc gtattgcgtt cagcgtgccg aaggattccc
     4381 acgttcgcct gagcgtcgtc gacgtgcagg gccggccggt ggccaccctc atcgacgaca
     4441 cgaaggcggc cggcagctac caggccacct ggtcgggcga ctccgaccgg aatggccgcg
     4501 cgccgtccgg catctacttc atcctgatgg aggccccggg gaccaagctg gtccagcgcg
     4561 tcgcgctggt ccactgatcg ataggcgtcc gtaacgaaca ccggcggcgg ggaggggcaa
     4621 ccctctccgc cgcttcgttt tgggtcgcgc cgatccctcg atagaggcgt tggaggtggt
     4681 ccagatgagg gggggactcc cacaaagtga cgtcattttt caatattgac atcacttctc
     4741 cgcaaggcta gaattcctgt atgtcaatca aatcattcac cttacgaatt gatccgcttc
     4801 tccgccgcca attggacgaa ctggccgagg ccagcgacac ccccctgaac cgcctgatca
     4861 acgaggccct gcgcgacttc gtcggcaaga gaagcctggc gctggaaggg gagctgcaat
     4921 cacggatcga gcgcctgcgt gcgtatcgcc gacatgaccc gaaccatgag agggcaatcg
     4981 aagccttcgc gaaggccgag gggactgttc cggatccggt cgaggggcgg gctaccgcga
     5041 aatcaggacc cattcgaaag aagatcgtcc gcctgctgaa tggctgattg ggacgaagac
     5101 agtccgacac tccaggccaa tctcgtcggg gtacttcgcg agattcgcga ccgggtcggt
     5161 cggcgaaccg cactctccgt tgaggatgcc cgaggctggc actccgccat catgcagggc
     5221 ctgaaagtac cgaacccggc acatgtcgct cactttcgcg gagagcccgg tctgaaaagc
     5281 ctgggtgttc gggtcgggcg caggcgtggt actccgccgg aacgtgtggc taccgacctc
     5341 caatccttcg aagtcaccct tcaggccgcc gtcagtgagc tggatcggcg catcccacac
     5401 ggcgtccttc caaacgacga cggaatcgcg gccgtgatcg acctggcagc gtgggctcat
     5461 gccaagtggg tgagaattca tccgttcgcg aacgggaatg gacgcaccgc ccgactctgg
     5521 gccaacgcac tcctcatgcg gtacgggcta ccgccgttcg tccgactccg cccacgtccc
     5581 gatcacggtt acggaaacgc cggagccgca gccatgcagg ggaaatggga gccgaccgca
     5641 gccgcattcc acgcgatgct tggcgaattt ctcgagtcgg gggcttgagg gagggctcat
     5701 agtccgtgcc gccgactcat ctccaataga aaaaacagcc agctgaccgc aaggccagct
     5761 ggctgatcca cttcggatac gacgtcaaag cgggccgaaa tccgggtccc aacctacttc
     5821 ttcatcatct ccgccatctg cttgaagaac acctcggcgc gctggcccag ctcggcgccg
     5881 gtcaggtctt ccttgcgcgt ggcgatggtc caggcgaggc cgttcggatc tttgagagtg
     5941 ccgcagcgat cgccccagaa ctggtcctcg acggggcgga tctgggtggc gccggcggcg
     6001 atggccttgt tgaagagggc gtcgcagtcg gccacgtaaa tccagagcgc cacgggcgag
     6061 ccgccaaagc ccttcggtcc cttgccgtcc atcatggtgt cgtgggccat tagcatcgag
     6121 tcgccgatct tgaacgccga gtgcatgatc cggccgtccg gtcccggact cttcgaaatg
     6181 atctccgcgc ccagcccctt cacgtaccag tcgagcgccg cggaacactc ctcgaacgtg
     6241 aggatcgggg tgacggtgcg atatccctcg ggaacggggc tctttgcggg tgccatggaa
     6301 acctcctgtg gatcgatggt gacggtcacg ggtcagatga ttggaccgtt gaggttaggc
     6361 ccgggaaatg ggaggggcaa ggggcctctg acgcccttcc ggcagcctct ggcgcccgtc
     6421 tagcgagttg ccaccagcgt gttgtggaac gccgccacct gccactttcc cggggaaccg
     6481 gtcaggacca gtgaggccag cgaggcaagg gttccggcca tgggcccggc cggcacctcg
     6541 aggcgggcgt tgatgtgcgc cagcgccaca ccaggcgcca ggaggcgcgc gctcgccagc
     6601 tggtaatgca ccacgcttcc cttgtagatc gtgtcgaaga tcccctggtg cccccgtgca
     6661 atcgcctcac gcgtccgatg gtgatcggcg cgcacgttca cgaaatcggc atcctcggca
     6721 aacggcctgg cgaaggcggc gccatcggcg gcgttccagg cgcgctccag cgtggcgacc
     6781 aggtcggata tctcctgctg caggtcggac atcggtatct ccaatcgaaa gaaaggtcgg
     6841 cccccgtcca ccatcgtaca ttcccgaacg ggcgctttct gcactagaat tcccggccac
     6901 gggaggaatc atgtcacgca tcgacgtcac accctgcagc accctcgagg atctctcggc
     6961 ggcactccag ccgatcatgc actacttcgg cggcgggttc ggcgaggccg atctggcgcg
     7021 ctggtcgaag atcatcgaca tcccccgcat gcacgccgcc cgcgaggacg gccaggtcat
     7081 cggcggggcg ggcgccttca ccttcgagat gtcggtgccc gggggcacgg ttccgtcggc
     7141 cggcgtcacc gtcgtgggcg tgctccccac gcaccggcgg cgcggcgtcc tcaccgccat
     7201 gatgcgggcg cagctcgacg acgtgcatcg ccgcggcgac ccggtggcct ggctctgggc
     7261 ctccgaggaa acgatctacc gccagttcgg ctacggcatg gcctcgctct cgggcgaggt
     7321 ggaaataccg aagagcgcca tcgccttcga acggaacatc gagcgacgcg gcgtgacgcg
     7381 aatcgtttcc gacgaagagg cactggagcc gttcgcctcc atctacgaac gtgtccgccg
     7441 cgaacagccc ggcatgtttt cgcgcaccgc cgactggtgg aagttgcgtc ggctggccga
     7501 caccgaacgc ggccgcgccg gcggcgccgg catgttgaac cgcgtcctcc tcaccatcga
     7561 cggcaggccc gaggcgtacg ccctctaccg cgtgcaccag tcgttcgagg gggccgccac
     7621 caacggtttc ctcaacgtca tcgaggccgt cggcgccacc gaccaggcca cacgcgaaat
     7681 ctggcgctac ctgctcgacc tggactgggt ggcgcgcgtc aaggccgcgc agctcccggt
     7741 cgatcatccg ctcgtcttcc tcatggcccg tcctcgccac atgcgcatgc gcctgagcga
     7801 cgcactctgg gtgcgcctgg tggatgtccc gaaggccctg gccgctcggc gcctcacgcc
     7861 gggggaagcg gtcgtcatcg acgtgcgcga ttccttctgc ccatggaacg ccgggcgcta
     7921 ccggatcgat ggtcgcagcg tggaacgcac cgaggccgcg ccggacctct ccgtggaggt
     7981 caacgcgctc ggttcggtgt acctcggcgg tttcacgttc ggccagctcg cgagggcggg
     8041 ggcgatcgag gtgttgcgcc ccggcggcgt gatgcgggcg gacgcgctct tcccgcgcga
     8101 gcgcgcgccg tggtgtccgg agattttcta ggcgcgcggc aatcggcggc gcgctcctcg
     8161 ccgcgttcgg cgaagcgata caataaagcc cgctccgccg ctctcccggc cgcccttgcc
     8221 agccgttccc cgccggtgcg gagccgcggg cgaatgtcct cagcgagtcg tgtggagata
     8281 acgccacacg tgggtcgatc caccgggcgc atgcatgcgc agaaaataga gtcccatcgg
     8341 aagcgcgccc acactgaggt cgagctgggt ggcgccggcc gacagggagt actgccagcg
     8401 agccacggcc cgtccggtca cgtcgcacaa ttcgaaggac gccgggccgg agtcgatccc
     8461 atcgagggac agacgcaggt tgcccgcaga gcccaaccga aggccgccgc cggcgccgtt
     8521 ccgattcggc aagccgggat tctggcttgc gtcgcacgtg gtggatacga ataatgcccc
     8581 gccgtccaca caacgggaat tcgctccgcg gtcgtgcgga atacagacac tggcggatcc
     8641 ctgacagctt ccgccatgtc catccgtcgc ggtgaagctc aggtgataca cccttccgtc
     8701 gccatttcca tttcgctcat cgcgtaccga tacctggccg gcggtgattc gagcgtccgg
     8761 acagctccgt ccggaagagg gtgatagagg gtcttcgtcc tgccggatgc cggtgacttc
     8821 aacgtccacc gcatcgccgt caggatccac gatccccgca agacttacgt ttcggaaacc
     8881 atcggcgttc ggtttcgatg cgatggccgt cgcgctcccg cattccgggg cgcggttgac
     8941 gatcagcgtc gccgtgcccg taactgccgc atcgcagcgg tccctggcgc ggaacgtgag
     9001 cgtgtatctt ccggcatccg ctacgccggg cgttcccgcc acggtcaaca cgacttccgg
     9061 gccggtggcg ggtagcgagg gcgtcacctc aagaaagccc ggcacgggtg agagcgtttc
     9121 gacgacgaga tcgtcgcgga gcccgttggg atcctggaat cgccacgaga gttcgaacgg
     9181 ttcacccacg gtcaccgtga tctccgaacc ggaagatgga ggcgtttgtg tcggggggcg
     9241 attttccgtg ccgcacaccc ggccgtcggt cgtgacgcgc tgcagcagga tggttgtttg
     9301 gatggcgcgc caggcggcat agaacccacc gcgtccgtcc ggcgcataga cggttctgcc
     9361 gtccggagag cggccggaaa cgtagatgaa ctttccttca ggcggccaat tcgccgcgta
     9421 ggtgccgacc ggatcaaagt gctgggcatg cacgaagttg ttcactgaag cgacgatcaa
     9481 cccacctttg ccgtccggca gggcccggca cagacttgag tggccggaca gatcggcctg
     9541 caaaggcgtg ccgttgatcg tccagcccgg taccggctgg cccgactcat ccaggcgggt
     9601 tgctcgtccc ggccagtagc cgcaattcgt gcaggcctgg ctggaccagg cgaagtaggc
     9661 gccaccttcg ccatccggca acaggtcggg gtcgtactgc tcgcccgagg tgaaaccgac
     9721 gattttcccg ccttgcaacc atcccgccgc cagcgttccg tcattgcgca gtcgcaccgc
     9781 ccgaatatcc cagcggttgg ccaggaagaa tcgaccatcc atccaggcga cgaagcaacc
     9841 ccccgtgccg tcaggcacga tcttcgggcg aattccgaca gtggcagccg tcgaaattcc
     9901 ttcagcgggc cacccgggaa gcggcgtccc gttgatcccg atccggtgga ctcggatctg
     9961 gccttcccgg agcgggcggg ccacgaagag cccacgcatg ccgtccgggg ccaggacagg
    10021 accctcgtag gtatcgatct gaccgatgct cgatccgttg accggccagg ctggatcgag
    10081 cgtgccattg cgcaggatcc gggtcaggta ggcgtcaccc gatgggtatc cgccgagcgg
    10141 acgcgtgtca tgccatccga taaagaatcc gccatcgtca tcgcgaagaa cgctcagagc
    10201 atattcctga gtgggatatc gacagatcgg caggccaccg accggccaat ccggcgccag
    10261 tgtgccatca gggaggatgt gtttcgcgta gatgtcgctg aaattctggc cgcccgggtg
    10321 acggtggtcc aaccaggtga caatgactcc cccctcggta tcgagcccga gagcggccgg
    10381 gtgactgtcc gcaccatcct ccgagatgag cagcccctcg atcggccaac cgggagccac
    10441 ctcgcccgac ccgaggaagc gttggagtcg gagcccggat ccatcccagg caagatagag
    10501 atcatccgag gcaccgggaa tcatggccca aagctgcttg aatcccggaa gtgatcctac
    10561 tgggttggcg tccggaaacc aggcggcgga agcggggaag gcaagcatga gcagaatcaa
    10621 ggaggcgggg cacaag
//
LOCUS       DASVOD010000716         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_257050, whole genome shotgun sequence.
ACCESSION   DASVOD010000716 DASVOD010000000
VERSION     DASVOD010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257050"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VF720_15420"
     CDS             <1..1777
                     /locus_tag="VF720_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX7880802.1"
                     /translation="TARAVSKSLKAPRERLVGLDYLTLEPESWSVVESLRAPSLDGIE
                     PYRLHLKAAEHSLRRGFDQLVSLDHLHDVERFPYQIKACLRVLRDMKGRSLLADEVGL
                     GKTIEASVVLKEYIMRGLVHRALILVPASLTTQWREEILNKFHLNFAVHGRREGWSDA
                     PFIIASLDTAKMSRNRDQILKGKYDLVIVDEAHRLRNHQTLAWKFVSQLSARYLLLLT
                     ATPVQNDLRELYNLVTLLKPGTLGTYRSFRRNFMVRGDKRLPKNTTDLSQLLQKVMIR
                     TTRSSTAIQFPKRRVQTLTFPLSPEEKALYEGVTEFVRRRIAAARGADFPRWHFVAMV
                     LQKEIGSSTRAAVRTLERARRDARYRAEWDDIDRLLALASEVGDGAKLAGLKDVLRRT
                     KDKVIVFTQFRATLEYLKEHVVADGDTVVLFHGNLTAAEKEAAIDDFRERSRILLSTE
                     AGGEGRNLQFCQTVVNWDLPWNPMKVEQRIGRVHRLGQTRDINIINFASEETVESYVL
                     EILHKKINMFELVIGEMDMILGHFSEENTFEDTVFKIWSGSAGTNEARRRFQELGDRM
                     LVARKRHEAVRDMDSRIFDHDDADR"
     gene            1764..2846
                     /locus_tag="VF720_15425"
     CDS             1764..2846
                     /locus_tag="VF720_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880803.1"
                     /translation="MRIAELEELIRVSLTSLGAEETLREAGIVHFRLDDELAGRFGRE
                     RLDLTFRPAVAAHHPEVELISAGSFMYDLILRLVRERGRAASGWLPIDEAIDPEARIH
                     KAVPRLAGRALTRVKSTWGTIYLFTFRLGFYSDLPHERLYTVRVDWESAKVRHDVHPW
                     RLVDAAGPPPEGAPGTPEGVEPEKAFRMAWVKVEEEVARLAEKHRRQAQDRLTEEVET
                     IESYYRQLIEEEKSVREQRVTRKGREESDAKIETLKLEWDRRVLEEKRRLSPDVKVAL
                     SCALKLRAPLDKWRAKTPPGRREPMVDYWIDRHTGDVWTVRRRKRGPNVAPKRLKGED
                     EPGIIDRPPDAAFGGPDDPEPEETPA"
     gene            2843..>3346
                     /locus_tag="VF720_15430"
     CDS             2843..>3346
                     /locus_tag="VF720_15430"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880804.1"
                     /translation="MSRQKLLRTEPHHDDALQAASLKAYEIAREQGRNLAIGAGVVVA
                     IVLGAIFFESNRKSSDAAASSALALAMSDVNANQFDAAALKLNDIVTRYGGSDSGNRA
                     RLYLGDIELRRGNAVAAQSQFDAFLAKTGKGDFFWSIGQRGKAVALENQAKYAEAAAA
                     FEALAGAS"
BASE COUNT          609 a         1143 c         1092 g          502 t
ORIGIN      
        1 aaccgccagg gcggtatcca agagcctgaa ggcgccgcgc gagcggctcg tcgggctcga
       61 ctacctcacg ctggagccgg agagctggtc ggtggtggag tcgctccggg ccccctcgct
      121 cgacggcatc gaaccgtacc ggctccacct caaggcggcg gagcactcgc tccggcgcgg
      181 cttcgaccag ctggtatcgc tcgaccacct gcacgacgtg gaacgattcc cgtatcagat
      241 caaggcctgc cttcgggtgc tgcgcgacat gaaggggcgc tcactcctgg ctgacgaagt
      301 gggcctcggc aagacgatcg aggcctcggt ggtcctcaag gaatacatca tgcgcggcct
      361 ggtgcaccgg gcgctcatcc tggtgccggc ttccctcacc acgcagtggc gggaggagat
      421 tctcaacaaa ttccacctga atttcgccgt gcacgggcgg cgggaagggt ggagcgacgc
      481 cccgttcatc atcgccagcc tcgacacggc caagatgtca cgcaatcgcg accagatcct
      541 gaagggaaaa tacgacctgg tcatcgtcga cgaggcccat cgcctgcgca atcaccagac
      601 gctggcgtgg aagttcgtga gccagctctc ggcccgctac ctcctgctcc tcacggccac
      661 gccggtgcag aacgacctgc gtgagctcta caacctggtc acgctcctga agccgggcac
      721 gctcggcacc taccggagtt tccggcgaaa cttcatggtc cgcggcgaca aacgcctccc
      781 caagaacact accgacctct cgcagctgct gcagaaggtg atgatccgca ccacgcgctc
      841 cagcaccgcc atccagttcc cgaaacggcg tgtgcagacg ctcacgtttc cgctcagccc
      901 cgaggagaag gcgctgtacg agggggtcac cgagttcgtc cggcggcgca tcgcggccgc
      961 ccggggggcc gacttcccgc gctggcactt cgtggccatg gtgctccaga aggagatcgg
     1021 cagttccacg cgcgccgccg tccgcacgct cgagcgcgcc cgcagggatg cccgctatcg
     1081 ggccgagtgg gacgacatcg accggctcct ggctttggcc agcgaggtgg gggacggcgc
     1141 caagctggcc gggctcaagg acgtgctgcg caggacgaag gacaaggtga tcgtcttcac
     1201 gcagttccgg gccaccctgg aatacctgaa agagcatgtc gtggcggatg gcgacaccgt
     1261 cgtgctgttc cacggaaacc tgaccgccgc cgagaaggaa gccgccatcg acgacttccg
     1321 cgagcgctca cggatccttc tctccaccga agccggcggc gaagggcgca acctccagtt
     1381 ctgccagacg gtcgtcaact gggatctccc ctggaatccc atgaaggtgg aacagcgcat
     1441 cggtcgcgtg caccgtctgg gccagacgcg cgacatcaac atcatcaact ttgccagcga
     1501 ggagacggtc gagtcgtacg tcctcgaaat cctccacaag aagatcaaca tgttcgagct
     1561 ggtcatcggc gagatggaca tgatcctcgg ccacttctcc gaagaaaaca cgttcgagga
     1621 cacggtcttc aagatctggt ccggctcggc gggcaccaac gaggctcgcc gccgcttcca
     1681 ggaactgggg gaccggatgc tggtggcccg caagcgacac gaagcggtgc gtgacatgga
     1741 ctcccggatt ttcgaccacg acgatgcgga tcgctgagct cgaggagctg atccgggtca
     1801 gcctgacctc gctcggcgcc gaggagacgc tgcgcgaggc cggcatcgtc catttccgcc
     1861 tggatgacga gctggccggc cgcttcggac gcgagcgcct cgacctgacg ttccgccccg
     1921 cggtggcggc gcaccatccc gaggtggagc tgatctcggc cgggtcgttc atgtacgacc
     1981 tcatattgcg gcttgtccgc gaacgcggac gggccgcctc cggctggttg ccgatcgacg
     2041 aggcgatcga tcccgaggcg cgcatccaca aggccgtgcc gcgcctcgcc gggcgagcgc
     2101 tcaccagggt caagtcgacc tggggcacca tctacctctt cacgttccgg ctcggtttct
     2161 actcggacct gccgcacgaa cgtctctaca cggtccgggt cgactgggaa tcggcgaagg
     2221 tgcggcacga cgtccatccg tggcgcctgg tcgacgcggc cggcccgccc cccgagggag
     2281 cgcccggaac gcccgaggga gtggagcccg agaaggcctt ccggatggcc tgggtaaagg
     2341 tcgaggaaga ggtggcccgt ctcgcggaga agcatcgtcg ccaggcccag gaccggctca
     2401 ccgaagaggt ggagacgatc gagtcgtact accgccagct gatcgaggag gagaagtcgg
     2461 tccgcgagca gcgcgtgacg cggaaggggc gggaggaatc ggacgccaag atcgagacgc
     2521 tcaagctcga gtgggaccgc cgcgtgctcg aggagaagcg gcgcctctcg cccgacgtga
     2581 aggtggccct gtcgtgcgcc ctgaaactgc gcgcgcccct cgacaagtgg cgggccaaaa
     2641 ctcccccggg ccggcgggaa ccgatggtgg actactggat cgatcgccac accggagatg
     2701 tctggacggt ccgccgccgc aagcggggcc cgaacgtggc accgaagcga ttgaaagggg
     2761 aggacgaacc gggcataatc gaccgtccgc ccgacgccgc cttcggcggc cccgatgacc
     2821 ccgaacccga ggagaccccg gcgtgagccg tcagaaactg ctacggaccg agccccacca
     2881 cgatgatgcc cttcaggccg ccagcctcaa ggcctacgag atcgcccgcg agcaggggcg
     2941 taacctcgcc atcggcgcgg gcgtggtggt ggccatcgtg cttggcgcca tcttcttcga
     3001 atcgaaccgc aagtcttcgg acgcggccgc ctccagcgcg ctcgccctcg ccatgtccga
     3061 cgtcaatgcc aaccagttcg acgcagcggc cctgaagctg aacgacatcg tgacccgtta
     3121 cggcggctcc gactcgggta accgggcccg cctctacctc ggtgacatcg agctccgccg
     3181 cggcaacgcc gttgccgccc agtcgcagtt cgacgctttc ctcgccaaaa ccggcaaggg
     3241 ggacttcttc tggtcgatcg gccagcgcgg caaggccgtg gccctcgaga accaggccaa
     3301 gtatgccgag gccgccgccg cgttcgaggc gctcgccggc gcctca
//
LOCUS       DASVOD010000717         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_308453, whole genome shotgun sequence.
ACCESSION   DASVOD010000717 DASVOD010000000
VERSION     DASVOD010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308453"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VF720_15435"
     CDS             <1..516
                     /locus_tag="VF720_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880805.1"
                     /translation="ISGRLPGAPGSEASTGESDKGSGIRAAEPETPGFDRNRDIYKEP
                     DPGKTPTAEALSWSSDFGFSWNGIRSEGHVKESALVDGRLTAQISRNWGMSYSTQYSF
                     DSKSLVAQDLRLTRQLHCWEAQFSYQATGETSEYSFRINVKSLPDIKFESGPGLGTSD
                     FLGRVSTGGGF"
     gene            687..845
                     /locus_tag="VF720_15440"
     CDS             687..845
                     /locus_tag="VF720_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880806.1"
                     /translation="MARSRSLTLMVALAGLAVAVGFGSRHAFSQGNTTELVVLSTASQ
                     NGELAPCG"
     gene            996..2087
                     /locus_tag="VF720_15445"
     CDS             996..2087
                     /locus_tag="VF720_15445"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX7880807.1"
                     /translation="MAEGLARLKYDAWTPGETEMYYGIANLLKFAEAMKCDAVSANVM
                     DGNGKPIFKDHVIKTVGGIRIGITGVTSKTIFESVPVAGNLGAKDYQFLDPIESLKPI
                     VNAMQNETDMVLVLAHVGAGDARRLAEEVPGIDAIVAGHRPGYMPSPDRVGDVVIMRA
                     GDRGQYGAKLNLTFDASKAMVDYKGTADALNESYPADATIGNDVQKFLDGLAKSEAEQ
                     NRKNQINAANNGKDKFLGTEICARCHSDIYTTWSKGPHASAFQTLVAANKQTDRSCIG
                     CHVTGWGDPTGYQTLVYRTDSAGKPDTTDAVEFRNVQCESCHGKGTMHGTAGLTAKVG
                     EEACVTCHDKANDPDFDFKKAIAQGLHHH"
     gene            2172..3590
                     /locus_tag="VF720_15450"
     CDS             2172..3590
                     /locus_tag="VF720_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX7880808.1"
                     /translation="MRRRSLVWLLLIFFVFLSASVGESHGPLPGRLLIVASARLHGAL
                     RSGAPCHHEYGGLAMRAAYHDELRAGEPATIAVDAGDFVAGAHVDNPRALAGALMMAR
                     TMGMVGYEAWTPGESELGYGLARLIEFDTGLGNTGVSANLRNAAGQPILKRWTVRNLA
                     GVRVGITGVIDPELLRGPELHGQAELTSRVIQQDFFVDEPSAALAAAVRTLRPRVDVI
                     VVLAHAKPFRAAALVENISGIDVVVVGHTSPMEVLEPSEHPTLVAFPSDRSMAHLIRL
                     ELDAMNRPGNISLSSTRLGSCHAPAESGDSTIVRAIDEFERHEAALVRRASLESSEAS
                     ALGSASRYLGGEVCARCHADVAAAWRTGAHARAFEPLVRSGRERDESCLPCHVTAWQE
                     PGGYTHLTGDDFDLAREDDPNARPPLAGVQCEACHGQGALHGTRRMVTQVSEEACRSC
                     HDAVNDPHFDYAKAVAGGFHHR"
     gene            3641..3784
                     /locus_tag="VF720_15455"
     CDS             3641..3784
                     /locus_tag="VF720_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880809.1"
                     /translation="MNRFRYLLLAPLLLAPLACGKGGSPGAPKPIIIIGSSSIWGEVS
                     PCG"
     gene            3863..>4453
                     /locus_tag="VF720_15460"
     CDS             3863..>4453
                     /locus_tag="VF720_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880810.1"
                     /translation="MDAGDLGQPDNPRVPGGWEVTEFTAKTLGEIGYDAWTPGERELL
                     HGPEKLKRLVSSFGVPAVSANIRMDGKRLFDEKVIRKTGGVSVGITGVTGPEVLLDGK
                     GISEAGAALAANFELTDPVRALKPVVAELQKKCDVVVVLAHLAPPEARHLAEEVPGID
                     VLVVGHVPGSGYEGERVGDTWVLRTGQRGQMVSFLDM"
BASE COUNT          805 a         1452 c         1461 g          735 t
ORIGIN      
        1 atttcgggac gccttcccgg agccccgggg agcgaggcct cgacgggcga gagcgacaag
       61 gggagcggca tccgcgcggc cgagccggag acgcccgggt tcgaccggaa ccgggacatc
      121 tacaaggaac cggatccggg gaagaccccc acggcggaag ccctcagctg gagcagcgac
      181 ttcggattct cgtggaacgg catccgctcg gaagggcatg tgaaggagag cgcgttggtg
      241 gatggccgtc tcaccgcgca gatttcgcgc aactggggga tgtcgtacag cacgcagtac
      301 tccttcgatt cgaaaagcct ggttgcccag gatctccggc ttacccggca actccactgc
      361 tgggaggcgc agttttccta ccaggccacg ggagagacct cggaatactc cttccgaatc
      421 aacgtcaagt cgcttcccga catcaagttc gagagcggcc cgggcctcgg gaccagcgat
      481 ttcctcgggc gggtgtcgac ggggggcggt ttctagagta tcgggtgata aacgccggga
      541 acccgcttcc cagcctcgtg tttcacggcg cctaagcgca atccctgaga gaccttgcgc
      601 cgtcgcgtga aagttcgcta tactgttgcc gcgttgaatt cactcggcgc aaacgcaatc
      661 accccaacat cttaccggag gaagtcgtgg cgcgttcgcg ttcactgaca ctcatggtgg
      721 ccctcgccgg tctggcggtg gcggtcggct tcggaagccg gcacgccttc agccagggga
      781 atacgacgga gctcgtggtt ctgagcaccg cctcccagaa cggcgagctg gccccctgtg
      841 gctgacacaa gaatcagcta ggcggacttg cccggcgggc aggtttttat cagcggattc
      901 ggcaggaaaa tccctacgtc ctcgtcgtgg acgcgggcga cttcggtatt cagaagccgc
      961 cgggcgccgc gggtgggctg gagaccagcc tcttcatggc cgaaggtctc gctcgcctca
     1021 agtacgacgc gtggacgccg ggtgaaaccg agatgtatta cggcatcgcc aacctcctca
     1081 agttcgcgga ggcgatgaag tgcgacgcgg tgtccgccaa cgtcatggac ggcaacggca
     1141 agccgatctt caaggatcac gtcatcaaga cggtgggcgg gatcaggatc ggcatcaccg
     1201 gcgtcacctc caagaccatc ttcgaaagcg tgccggtcgc cggaaacctc ggcgccaagg
     1261 actaccagtt cctcgatccg atcgagagcc tgaagccgat cgtcaacgcc atgcagaacg
     1321 aaaccgacat ggtgctggtc ctggcccacg tcggcgcggg cgatgcccgt cgcctggcgg
     1381 aagaggtgcc ggggatcgac gccatcgtcg ccggccaccg ccccggctac atgccgagcc
     1441 cggaccgggt gggcgatgtg gtcatcatgc gtgccggcga tcgcggccag tacggcgcca
     1501 agctcaacct taccttcgac gccagcaagg ccatggtcga ctacaagggc accgccgatg
     1561 cgctcaacga gagctacccg gctgacgcca cgatcggcaa tgacgtccag aagttcctcg
     1621 acggcctggc caagtccgaa gccgagcaga accgcaagaa ccagatcaac gcggccaaca
     1681 acggcaagga caagttcctc ggcaccgaga tctgcgcccg ctgtcattcc gacatctaca
     1741 ccacctggtc gaaggggccg cacgcatcgg ccttccagac cctggtagcc gccaacaagc
     1801 agaccgaccg ctcctgcatc ggctgccacg tcaccggctg gggcgatccg accggctatc
     1861 agacgctggt ctaccgcacg gattccgccg gcaagcccga caccaccgat gccgtcgaat
     1921 tccggaacgt ccagtgcgag tcgtgccacg gcaagggcac catgcacggc accgccggcc
     1981 tcaccgccaa ggtcggcgag gaagcctgcg tcacctgcca cgacaaggcc aacgaccccg
     2041 acttcgactt caagaaagcc atcgcccagg gtctgcatca ccactaaaca ggacccggga
     2101 cgacttgcga cgagtatgag aggccccggt cttccgaccg gggcctttta tgtcaccaca
     2161 tgggggttga gatgcgccgc cgatccctcg tctggctact cttgatcttc ttcgtctttc
     2221 tctccgccag tgtcggggag agtcacggtc ctctcccggg tcgactgctg atcgtcgcct
     2281 ccgccaggct tcacggcgca cttcgcagcg gggctccttg tcatcacgaa tacggcgggc
     2341 ttgccatgcg ggcggcgtat cacgacgagt tgcgagccgg ggaaccggcc acgatcgccg
     2401 tcgatgcggg tgacttcgtc gccggcgcgc atgtcgataa cccccgggcg ctggccgggg
     2461 cgctcatgat ggcgcggacg atggggatgg tcggatacga ggcttggact cccggtgagt
     2521 cggaactcgg ctacggcctc gcgcgactga tcgagtttga cacgggattg gggaacaccg
     2581 gggtatccgc caaccttcgc aatgcggccg gtcagccgat cctcaagcgc tggaccgtcc
     2641 ggaatctcgc cggcgtccgg gtcggcatca ccggcgtcat tgatccggag ctacttcgtg
     2701 gaccggagct ccacggtcag gcagaactca cgtcccgcgt catccaacag gattttttcg
     2761 tcgacgaacc atcggccgcg ctggccgcgg ccgtccgaac gcttcgtccc cgtgtggatg
     2821 tcatcgtcgt cctcgcccac gccaaaccct tccgtgcggc ggccctcgtc gagaacattt
     2881 ccggcatcga cgtcgtggtg gtggggcaca cgtcgcccat ggaggtgctg gagccctccg
     2941 aacatccaac gctggtcgca ttcccttccg atcgatcgat ggcccatctc atccggctcg
     3001 aactcgacgc gatgaatcga cctggaaaca tctccctgtc gtccacgcgg ctcggatcgt
     3061 gccatgcgcc agcggaatcg ggcgattcga cgatcgtcag agccatcgac gagttcgaac
     3121 ggcatgaagc cgcgctggtc cgccgcgcgt ctctcgaatc gagcgaggcc tcggcgctgg
     3181 gttccgccag ccggtacctg gggggcgagg tctgcgcccg atgtcacgcc gacgtggcgg
     3241 ctgcctggcg gactggcgca catgcacgag ccttcgagcc gctggttcgg tcggggcgtg
     3301 agcgggacga atcgtgcctg ccgtgccacg tgacagcctg gcaggaaccc gggggttata
     3361 cgcacctcac cggtgatgac ttcgacctcg cgcgggagga cgacccgaac gcgcgacccc
     3421 cgctggcagg cgtgcaatgc gaggcctgcc acggccaggg agcgctccac ggcacccgac
     3481 ggatggtgac gcaggtatcc gaagaagcgt gccgaagctg ccacgacgcc gtcaacgatc
     3541 cccatttcga ctacgcgaag gcagtggcgg gtggatttca tcaccggtaa gggctcggaa
     3601 accgggcgcc ggtaagtctc ttccgctaca atcatccccc atgaatcgct tccgttacct
     3661 cctcctcgca cctctcctcc tcgcccccct ggcctgcggc aagggcggct cgcccggggc
     3721 gccgaagccg atcatcatca tcgggtcgag ttcgatatgg ggagaggtgt cgccctgcgg
     3781 ctgatcgcac caggcgggtg gactggcccg gcgggccagc ctctataaga cgatccggaa
     3841 ggagaatcca gcggtcatca cggtggatgc cggcgacctg gggcagccgg acaatccccg
     3901 cgtgccgggg ggctgggagg tgaccgagtt caccgcgaaa acactcggcg agatcgggta
     3961 cgacgcctgg actccggggg agcgggagct tctgcacgga ccggagaaac tgaagaggct
     4021 cgtgtcctct tttggtgtgc cggccgtgtc ggccaacatc cggatggacg gcaagcggct
     4081 cttcgacgag aaggtcatca ggaagaccgg cggcgtgtcg gtcgggatca cgggggtcac
     4141 cgggccggaa gtgctgctcg acgggaaggg gatatccgag gcgggtgcgg ccctggcggc
     4201 caatttcgaa ctgaccgacc cggtgcgcgc cttgaagccg gtcgtggccg aactgcagaa
     4261 gaagtgcgac gtggtcgtcg tgctggctca tctcgccccg cccgaggcgc gccacctggc
     4321 cgaggaagtg cccgggatcg acgtgctggt ggtggggcat gtgcccggtt cgggctacga
     4381 aggggagcgg gtgggcgata cctgggtgct gcgcaccggt cagcgcggcc agatggtgtc
     4441 gttcctcgac atg
//
LOCUS       DASVOD010000718         7732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_153798, whole genome shotgun sequence.
ACCESSION   DASVOD010000718 DASVOD010000000
VERSION     DASVOD010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153798"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF720_15465"
     CDS             <1..409
                     /locus_tag="VF720_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880811.1"
                     /translation="PDHWRRVERNGITLAMRTGADLVVVKLFALFHDAQRRNDGDDPE
                     HGARGAAYAAQLRNRWLDIDDWRFALLEQACTWHTAGRHHDDPTIGSCWDADRLDLTR
                     LGILPDPAYMSTPYGRELAAAGGSGRRRGGRGA"
     gene            complement(406..3078)
                     /locus_tag="VF720_15470"
     CDS             complement(406..3078)
                     /locus_tag="VF720_15470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7880812.1"
                     /translation="MSQDRWPRIDELFHRALMLPESERERFIDQAVDDEADVVLEVRR
                     LLQAHREAGTFFLDSPASAALADDDFTSFAGRQIGAYRLVREIGRGGMGSVYLAERAD
                     RQFEKRLAIKLVKRGMDSDSVLRRFRAERQILASLDHPNIAGLLDGGTTDDGLPYFVM
                     ELVEGRPIDEYAETKGLSIEARLELFRQVCAAVSYAHQHLVIHRDLKPSNIHVTNDGT
                     VKLLDFGIARILGSGEPDVPSTVTVLGLMTPEYASPEQIQGRPTTTLSDVYALGLVLY
                     RLLTGAAPYRLESRSPEELARVIGGVEPMRPSTAGAGTRRLRGDLDNIVLKALRKDPV
                     RRYASVDQFSEDIRRHLAGLPVIARPDTVRYRATKFVQRNQVAVIGAAVVFVTLVAGI
                     IATSWQARRARQHERIARTEQARAERRFQEVRELAHKVVFDYHDAIKDLPGATAVRER
                     LVRDALIYLDNLSQEAEDDTALLTELGNAYERVGDVQGGTLFANLGNTAGALESYRRA
                     LSLYEARRVITPRDVISRRDVATTHAMIAQLVWETGNTDSALTEYRLASEQLKPLVAE
                     LPGSDELKMSLAACQDYEGLILQDRGEIGPSIEAFRGALASQAARSDQNSESVRRATA
                     TAHEHLASALMSDNRADEALTEIGKSIALWEGLMREFPDNAGHQRGVGVGYYFEGNFL
                     ATLGRAEASLESSRKYLAISERLARIDPDNEQYRGDQAFALVRIGDRLGELERLTESI
                     DAYERSREIRRKETIADPVNLWKKAALIESLAKLSRTYARAGQDVPAIREGEQAFELM
                     RTVEVEPRNAMIRGFLAETYGDLGEAHLRLAEHPSTASADRSSRWREARDLYRRSADI
                     WRDLENLGILSAEDAKKPVRVAEALEKCEAALKS"
     gene            complement(3075..3647)
                     /locus_tag="VF720_15475"
     CDS             complement(3075..3647)
                     /locus_tag="VF720_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7880813.1"
                     /translation="MQHDESAQVTGLLTGWCAGDKGVLDRLLPLVYDELRRIAARHLE
                     RERPDHTLQPTALVHEAYFRLIDQRKVDWRNRAQFLGLAAEMMRRILVNHARERSALK
                     RGGGFRKVSLTEALDTPGPEVDLAGLDEALRRLADLDSRKARVVELRFFGGLTNAEAA
                     EVLAVSEATIERDWSFARAWLFAALAESPS"
     gene            3811..>7732
                     /locus_tag="VF720_15480"
     CDS             3811..>7732
                     /locus_tag="VF720_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880814.1"
                     /translation="MPANSNTRRHLRRPLALMVVALLALAGPAGATVSPNPVKVRLEG
                     DSGAAEPGKPSSVVLSITAAADVRLEEVFLNTGRSWKLVDGEIPAVIEMSSGEVRRFP
                     ITVTGPDPADLLAFEYQVGNKSIRKPFDLSPARAADRRGRSLVAVTDPVALAGLEAGI
                     DAASRRPEAAPPRERGAHRNFESPVAAESADRATTASERDIRVVGWFTYNRMDGGAAV
                     GVDGTTARVYDEDSTWDELLATQVLGPDGRLDVTFHWDPCFACDGTPDIYVEFELDNS
                     RVQVQEEGLFEVDYTWETGVWNDFGGSFLDVGAQMPSNGNLMPACHIMVNAMRAWRFA
                     AGTGRDCPMIDVQWPDDDDCPTGCYNNYFEEIHIPTSRQWDESTIVHEYGHHFTKTFV
                     SYTEPDYCNNFCDTNVSEEECGHCLWCRETDHDAWNEGFPYYFSDVIIRTYQSMYGRG
                     PVTPRINFETLARCSQDNIFATPNLTEGFLAAMLVDIDDSAQDNDPWGGSGIDELSLG
                     FDEILGIADIRAPNNPQDFINWFRMDYPGEKEKFWATALNNGFQTDFAPPGKVTNITS
                     DHVLNQDWMTNPNIKWFWSRASDDASGIAGYSIWVQRNIIPGMPDAVAEVGDVTQYVS
                     GPHPPGWYWIAIRAVDRNGVWSNDYELQGPYGIRAQEPANLRVQHPSNWSGLGVVPRH
                     VSGASEFDCPAPTFLNGEGGLTYFNAIYNNAGEQATWVSQYWNSLYLDGYPFLFQHRI
                     NPLPAGSYDWMLNQPATITGGRHTLGVRIDDTDLMLETDETDNMWAHQWAWSPPWIGL
                     WAPGYYNSPPQRYAGTEWIVDGSFYTANCRGIRGSDQIVYPNQMWTAVTLHATDGADY
                     DMGAYAASNDPNWAWTNLYASSVRGANATDIVLRNSWQGTGSNLWYDLGVTNYSGGVP
                     GVNGFYMQEVTSSTVAFNSTTMGSLGTQEMLKLYQFTSPGGQIFARLQVSDPSQGRLY
                     LTMIPMLAPYFGNVLGAGTLAVTGESTGEGWISEAQDGFPCVAVWRDPTMGTGPISFT
                     LTLMQAPSDMVMRTPAGWAGPLVPRPANDGVPGAVPAPALLIGDASQTWINYAIGNDH
                     AVPPNPTVTSNIFRDDFLLLADPASAPASGGTTYRNLPTAYGVPGGRHVLGIQLDAAS
                     QFPEVSESNNATAAQWVWRPAALPFDITMSRSAPPAPFAGWSRMPAGSPLWFNMDGVR
                     TTAWTPQAGGYGWKVAALAMPTAGSDVDLALFEASSNATTGFDNELASSRWGIDQSDF
                     VIADFRVTTPRQFDVGFTAPVVQSGSFSLEFRRSTYLGSGPAAPG"
BASE COUNT         1249 a         2602 c         2606 g         1275 t
ORIGIN      
        1 ccctgatcac tggcgacgcg tcgagcggaa tggcatcaca ctggcgatgc gaaccggagc
       61 ggatctcgtg gtcgtgaaac tctttgcgct gttccacgac gcgcagcgcc ggaacgacgg
      121 cgacgatccg gaacatgggg cacggggcgc ggcgtacgcg gcgcaactga ggaaccgctg
      181 gctcgacatc gatgattggc ggttcgcgct gctcgaacag gcgtgcacct ggcacacggc
      241 cggccggcat catgacgacc cgaccatcgg gagctgctgg gacgcggacc ggctcgacct
      301 gacacggctg gggatcctgc ccgatccggc gtacatgagc acgccgtacg ggcgcgagct
      361 ggctgcagcg ggagggtcgg ggcgacggcg cggcggccgc ggcgcctagg acttcagcgc
      421 cgcctcgcac ttctcgagcg cctcggcaac gcgaaccggt ttcttcgcgt cctccgcact
      481 caagattccg aggttttcga ggtcgcgcca gatgtccgcg ctgcgccggt agagatcgcg
      541 cgcttcgcgc cagcgtgacg agcgatcggc cgacgccgtt gacgggtgct cggcgagccg
      601 caggtgcgcc tcgccgagat cgccgtaggt ttcggcgagg aagccgcgga tcatcgcgtt
      661 gcgcggttcg acctcgacgg tcctcatcag ctcgaacgcc tgctcgcctt ccctgatggc
      721 ggggacgtcc tggccggcgc gcgcgtacgt tcgcgagagc ttggcgagcg actcgatcag
      781 ggcggccttc ttccagaggt tgaccgggtc ggcgatggtc tccttccggc ggatctcgcg
      841 ggatcgttcg taggcgtcga tcgactcggt gagacgctcc agttcgccga gccggtcacc
      901 gatgcggacg agggcgaatg cctggtctcc ccggtactgt tcgttgtccg ggtcgatcct
      961 ggccagtctc tcggaaatcg cgaggtactt gcgcgagctc tcgagcgagg cttccgcccg
     1021 acccagggtg gccaggaaat tcccttcgaa gtagtagccg actcctacgc cccgctggtg
     1081 gcccgcattg tcggggaatt cccgcatgag gccctcccag agtgcgatcg atttcccgat
     1141 ctcggtcaac gcctcgtcgg cccggttgtc ggacatcaac gcactggcca ggtgttcatg
     1201 cgcggtggcg gtggcacgtc ggaccgattc actgttctgg tcggagcggg ccgcctggga
     1261 tgccagcgcc ccgcggaagg cttcgatgga tggtccgatt tcgcctcgat cctggaggat
     1321 caatccttcg tagtcctggc aggcggccag cgacatcttg agttcatcgc tcccgggcag
     1381 ctcggcgacc agcggcttca gctgctcgct ggccaggcgg tactccgtga gggcggagtc
     1441 cgtgttcccc gtttcccaga ccagctgggc gatcatggcg tgggtcgtgg ccacgtcgcg
     1501 acgcgagatg acgtcccgtg gcgtgatcac ccgccttgcc tcgtacagcg acagcgcccg
     1561 ccggtagctc tccagggctc cggccgtgtt cccgaggttg gcgaagagcg tccccccctg
     1621 gacatcgccg actcgctcgt acgcattgcc cagttcggtg agcagcgccg tatcgtcctc
     1681 cgcctcctgg ctcaggttgt cgagatagat gagcgcatcg cgcacgagtc gttcccggac
     1741 cgccgtcgcg cccggcaggt ccttgatggc gtcgtggtag tcgaagacga ccttgtgggc
     1801 cagctcacgg acttcctgga aacggcgctc ggcacgggcc tgctcggtgc gggcgatccg
     1861 ttcgtgctgt cttgctcggc gcgcctgcca cgacgtggcg atgatgccgg ccaccagcgt
     1921 gacgaagacg acggcggcgc cgattacggc aacctggttg cgctgcacga acttcgtggc
     1981 ccggtagcgg accgtgtcgg ggcgtgcaat gaccggcagg cccgcgaggt gccggcggat
     2041 gtcctcggaa aactggtcaa ccgaggcgta gcggcgaacc gggtccttgc ggagcgcctt
     2101 gagcacgatg ttgtcgagat cgccgcgcag gcgacgagta ccggcgccgg ccgtgctcgg
     2161 gcgcatcggc tcgacgccgc cgataacccg tgcgagctcc tcgggcgagc ggctctcgag
     2221 tcggtagggc gccgcacccg tcagcaaccg gtagaggacg aggccgagcg cgtagacatc
     2281 cgagagcgtc gtcgtcgggc gcccctggat ctgctcgggg ctcgcgtact ccggcgtcat
     2341 gaggccgagg accgtgaccg tcgacggcac gtccggctcg ccgctgccga ggatgcgggc
     2401 gatcccgaag tcgaggagct ttaccgtgcc gtcgttcgtc acgtggatgt tcgacggctt
     2461 caggtcccgg tggatgacca ggtgctggtg cgcgtacgag acggccgcgc agacctgccg
     2521 gaagagttcg aggcgtgcct cgatcgacag gcccttcgtc tcggcgtact cgtcgatcgg
     2581 gcgcccctcg accagctcca tgacgaagta ggggaggccg tcgtcggtcg tgccgccgtc
     2641 gaggaggccg gcgatgttgg ggtggtcgag cgaggccagg atctgacgct cggcgcggaa
     2701 ccgccgcagg accgaatcgc tgtccatccc gcgcttgacc agcttgatgg ccagccgttt
     2761 ctcgaactgc cggtcggccc gttccgccag gtagaccgat cccatgccgc cgcggccgat
     2821 ctcgcggacc aggcgatagg cgccaatctg gcggccggcg aacgaggtga agtcgtcgtc
     2881 ggcgagtgcg gcggaggccg gcgagtccag aaaaaaggtg cccgcctcgc ggtgcgcctg
     2941 gaggagccgc cggacttcca ggacgacgtc ggcctcgtca tcgaccgcct ggtcgatgaa
     3001 tcgctcccgc tccgactccg gcagcatcag cgcgcggtga aacagctcgt cgatgcgggg
     3061 ccagcgatcc tggctcacga cggcgactcc gcgagcgccg cgaagagcca ggcgcgcgcg
     3121 aagctccaat cacgctcgat ggtggcttcc gacacggcga gcacctccgc cgcctcggcg
     3181 ttggtcagtc cgccgaagaa acgcagttcg accacgcgcg ccttgcggga gtcgaggtcg
     3241 gccagtcgcc ggagcgcctc gtccaatccc gccaggtcga cctcgggacc gggcgtgtcg
     3301 agcgcctcgg tcaggctcac cttgcggaag cccccaccgc gcttcaacgc cgagcgctcc
     3361 cgcgcgtggt tcacgaggat gcgtcgcatc atctcggcgg ccaggccgag aaactgggcg
     3421 cggttgcgcc aatcgacctt tcgctggtcg atcagccgga agtacgcctc gtgcacgagg
     3481 gcggtgggct ggagcgtgtg gtccgggcgc tcccgctcga gatggcgggc ggcaatccgt
     3541 cgcagctcgt cgtagacgag gggaaggagc cggtcgagca cgcccttgtc ccccgcgcac
     3601 caaccggtca gcaaaccggt gacctgtgcc gactcgtcgt gctgcacccg ggatcctccg
     3661 accgatcgca ccatacggat ctggctcgag cctcttcgag tcggagtcta acagccgcga
     3721 tttcgcatga gggacatggc gtccggatcg ggcgtttcgg atcacgggaa atcccgtcga
     3781 tcccatgcgc tcgctctacg gaggttcgtc atgcccgcca attccaatac ccgccgtcac
     3841 ttgcgccgcc ccctggcgct catggtggtg gcccttctcg ccctggccgg accggcgggg
     3901 gccacggtca gcccgaatcc cgtcaaggtc cggctggaag gggattccgg ggccgccgag
     3961 cccgggaaac cgtcttcggt cgtcctttcc atcacggccg cggccgatgt ccgcctcgag
     4021 gaggtgttcc tgaacaccgg ccggagctgg aagctggtgg acggcgagat cccggccgtt
     4081 atcgagatgt cctccggcga ggtgcggcgc tttccgatca ccgtgacggg tccggatccg
     4141 gccgacctcc tggcattcga gtaccaggtg ggcaacaagt ccattcgcaa gccgttcgac
     4201 ctttcgcccg ccagggccgc cgatcgacgc ggccgatcgc tcgtggcggt gaccgacccg
     4261 gtggcgctcg ccgggctgga agcggggatc gatgcggctt cccggcgtcc cgaggctgct
     4321 cccccgcgcg agaggggggc ccaccggaac ttcgagtcgc cggttgccgc cgaatcggcc
     4381 gaccgggcca cgacggccag cgagcgcgac atccgcgtgg tgggatggtt cacctacaac
     4441 cgcatggatg gcggcgcggc cgtcggcgtg gacggcacca ccgcccgcgt gtacgacgag
     4501 gattcgacgt gggacgagct gctggccacg caggtcctcg gtccggacgg ccggctcgac
     4561 gtgacgttcc actgggatcc gtgcttcgcc tgcgacggaa cgccggacat ctacgtggag
     4621 ttcgagctcg acaactcgcg cgtgcaggtg caggaagagg ggctcttcga agtggactac
     4681 acgtgggaaa ccggcgtctg gaacgacttc ggcggcagct tcctggatgt cggggcgcag
     4741 atgccctcga acggcaacct gatgccggcc tgccacatca tggtgaacgc gatgcgcgcc
     4801 tggcgcttcg ccgccggcac tggccgcgac tgcccgatga tcgacgtgca gtggccggac
     4861 gacgacgact gccccaccgg ctgctacaac aattacttcg aggagatcca catccccacc
     4921 tcgcgccagt gggatgagag caccatcgtt cacgagtatg ggcaccattt cacgaagacg
     4981 ttcgtgtcct acaccgaacc cgactactgc aacaatttct gcgataccaa cgtgtccgaa
     5041 gaggagtgcg gtcactgcct gtggtgccgt gaaaccgacc acgacgcctg gaacgagggg
     5101 tttccgtact acttctccga cgtgatcatc cggacgtacc agtccatgta tggccgggga
     5161 ccggtgacac cacggatcaa cttcgagacg ctggcgcgct gcagccagga caacatcttc
     5221 gcaacgccca acctcacgga gggtttcctg gccgcgatgc tggtcgacat cgacgactcc
     5281 gcccaggaca acgatccctg gggcggatcc ggaatcgacg agctgtcgct cggcttcgac
     5341 gagatcctcg ggatcgccga catccgggcg cccaacaatc cccaggactt catcaactgg
     5401 ttccggatgg actatccggg agagaaggag aagttctggg ccacggcgct gaacaacggc
     5461 ttccagaccg acttcgcgcc gccggggaag gtgaccaaca tcacctccga ccacgtgctg
     5521 aaccaggact ggatgacgaa tccgaacatc aagtggttct ggagccgcgc gagcgacgac
     5581 gcctccggca tcgccggcta cagcatctgg gtgcagcgga acatcatccc cggcatgccc
     5641 gacgcggtgg ccgaggtggg cgacgtgacg cagtacgtca gcgggccgca tccgcccggg
     5701 tggtactgga tcgccatccg ggccgtcgac aggaacggcg tctggagcaa cgactacgaa
     5761 cttcaggggc cgtacggtat ccgcgcgcag gagccggcca acctccgcgt gcagcacccg
     5821 tcgaactggt cgggccttgg cgtggtgccc cggcacgtat cgggcgccag cgagttcgac
     5881 tgcccggccc cgacgttcct gaacggcgaa ggaggcctga cttacttcaa tgcgatctac
     5941 aacaacgccg gcgagcaggc gacgtgggtg tcgcagtact ggaattcgct ctacctcgac
     6001 ggctacccgt tcctcttcca gcatcggatc aatccgctgc cggcgggaag ctacgactgg
     6061 atgctcaacc agccggccac gattaccggc ggtcgtcaca cgctgggcgt tcgcatcgat
     6121 gacaccgacc tgatgctcga gacggacgag accgacaaca tgtgggcgca ccagtgggcg
     6181 tggtcgccac cgtggatcgg actgtgggca ccgggctact acaacagtcc gccgcagcgc
     6241 tacgccggca ccgagtggat cgtggacggt tccttctata ccgccaactg cagggggatc
     6301 cgcgggagcg accagatcgt ctacccgaac cagatgtgga cggccgtgac gctgcacgcc
     6361 accgacggcg ccgactacga catgggggcc tacgcggcga gcaacgaccc gaactgggcc
     6421 tggacgaatc tgtatgcctc gtcggtccgg ggtgccaacg caaccgacat cgtcctgcgc
     6481 aactcgtggc aggggacggg ttccaatctc tggtatgacc tcggggtcac caactattcc
     6541 ggtggcgtgc cgggcgtaaa cggcttctac atgcaggagg tgacgtcgag caccgtcgcc
     6601 ttcaacagca cgaccatggg gtccctggga acgcaggaga tgctcaagct ctatcaattc
     6661 acctcgccgg gcggacagat cttcgcccgg ttgcaggtgt ccgatccgtc ccaggggcgg
     6721 ctctacctga ccatgattcc gatgctggct ccctatttcg gcaacgtgct cggcgccggc
     6781 acactggcgg tgaccggcga atcgaccgga gaggggtgga tctccgaggc gcaggatgga
     6841 tttccctgcg tggcggtgtg gcgcgatccg accatgggga ccgggccgat ctcgttcacg
     6901 ctcaccctca tgcaggcgcc atccgacatg gtgatgcgga cgcccgccgg ctgggcggga
     6961 cccctggtgc cgcggccggc caatgacggc gtgcccgggg ccgttccggc ccccgccctg
     7021 ctgatcggcg acgcctcgca gacctggatc aactacgcga tcggcaacga ccacgcggtg
     7081 ccgcccaatc cgaccgtcac gtccaacatc ttccgggacg acttcctgct ccttgcggac
     7141 ccggcctcgg cacccgcttc gggagggacg acctaccgga atctgccgac cgcatacggg
     7201 gttcccggcg gacgacatgt actggggatt caactcgacg cggcgtccca gttccccgaa
     7261 gtcagcgaat cgaacaacgc aaccgccgcc caatgggtct ggcgcccggc ggcgctgccg
     7321 ttcgatatca ccatgtcacg atcggctccc cccgcgccct tcgccggctg gagccggatg
     7381 ccggccggct cgccgctctg gttcaacatg gacggcgtgc gcacgacagc ctggacgccg
     7441 caggccggcg gctacggctg gaaggtggcg gcgctggcca tgccgactgc cggatccgac
     7501 gtcgatctcg cactcttcga ggcgtcgtcg aacgccacga ccggcttcga caatgagctg
     7561 gcctcgtcgc gctggggaat cgaccagtcc gacttcgtca tcgccgactt ccgggtgacc
     7621 acgccgcgtc agttcgacgt gggattcacc gcgccggtcg tccagtcggg gagcttcagc
     7681 ctggagttcc ggcgctcgac atacctgggt tccggccccg cggcgccggg cg
//
LOCUS       DASVOD010000719         7676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_881546, whole genome shotgun sequence.
ACCESSION   DASVOD010000719 DASVOD010000000
VERSION     DASVOD010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7676
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881546"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VF720_15485"
     CDS             <1..1129
                     /locus_tag="VF720_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX7880815.1"
                     /translation="AEAMRNALDAAPANPSSVHAAGKAARRLVDGARRSVAGLVGGRP
                     EEIIFTGSGSEAAWIALHGALEAARDEDAPPLVVSSPIEHAALREPLAALDEAGVIRL
                     TLAPIGATGLVDADCFEEILADRPALVVCQWANNETGVIQPVDIIGTACRAAGVPFLV
                     DAVAAAGRLDLRATHGIADLVVLSSAKLGGPAGVGALWIRPGLSLTSPLGAGGQEQGL
                     RGGTENVIGIVGFGAAAAAAATTDVGGLAAVMAHGVSRVRERVSGALLAGDGSPRLAN
                     TAQFVVPHDDEEMLILALDRFGYAVSAGSACAAGAHKRSHVLEAMGLLGPGLASVRVS
                     IGADTTKGDMDDFAEALRACVRAAAPAEAEEIVEWPTGGAG"
     gene            1126..2211
                     /gene="mnmA"
                     /locus_tag="VF720_15490"
     CDS             1126..2211
                     /gene="mnmA"
                     /locus_tag="VF720_15490"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEX7880816.1"
                     /translation="MSKGLVVSAMSGGVDSAVAAALLREQGYDVYGVTMKLWCYQREA
                     PTARACCSMAAITEARSTADLLGIEHAVVDLEEQFEAHVIGPFAEDYQNGRTPNPCVN
                     CNTFIKFGTLLEKARRLGAGWVATGHYALMSHEESGPVMRRGVDDSKDQSYALWGLNR
                     KRLNRVLMPLGALTKTEVRERARALALPVADKPESMDICFVEGGHYADFLTNRLGAFA
                     QAEPGPMVDTSGKVVGTHQGVMRYTVGQRKGLGLARPEPTFVVGIDAATNTLTVGEES
                     GLYRRSFEAGRPNFVSMEHPTTPFRAEIRVRHRGRTIPGLVTPLAPGRLRIDLDQDER
                     AITPGQSAVFYDGDVLLGGALIERAID"
     gene            complement(2231..2320)
                     /locus_tag="VF720_15495"
     tRNA            complement(2231..2320)
                     /locus_tag="VF720_15495"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2284..2286),aa:Ser,seq:gct)
     gene            complement(2374..2790)
                     /locus_tag="VF720_15500"
     CDS             complement(2374..2790)
                     /locus_tag="VF720_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880817.1"
                     /translation="MTRSFLLAAAFLAVAGCSADEDDPAPPPCLVGCWEATNHDARVD
                     FRCYFEDGTGYFVFGDTCWNTTWASASDTTVRVESCWFIDVHRFECTENGNLLTREWG
                     RTEYRRVASPGCNATIPAEENCQGPDNAGRPTRSRR"
     gene            complement(2871..3305)
                     /locus_tag="VF720_15505"
     CDS             complement(2871..3305)
                     /locus_tag="VF720_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880818.1"
                     /translation="MFTVAGLVTMMALGAGMFSTAHAGFGIGGMFREDADFGIQGRFK
                     TAAGKKLMIVPQFGYFFDDEFFDADVDLHLDFKNSGKMNFYGLGGVDLIADSDFDNTE
                     FGINAGAGLSTPLGDSAKSLFFEAKYVFSDFDGFGLNFGIMF"
     gene            complement(3474..4007)
                     /locus_tag="VF720_15510"
     CDS             complement(3474..4007)
                     /locus_tag="VF720_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880819.1"
                     /translation="MSARSVTSSLFWIVALLPIAAGCGEDTPPSAPVRLIQGIRPGIP
                     VSVTDCNSAPCLCEEYPTAYEASVQAYHPTDYAGDGARLIDIQVPGTALVHIWPATAC
                     GDSMAAYAVTIGCPICLNISRDTEPEMLNRTFSIWPSPVTHKVLFHARTGLDYWVHQD
                     DRTPVHLGPTATSIEIP"
     gene            complement(4183..5985)
                     /locus_tag="VF720_15515"
     CDS             complement(4183..5985)
                     /locus_tag="VF720_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880820.1"
                     /translation="MTWRATSLLVALLAVPLLAGCHSFRKDLPRETVLSDRRVTAMQT
                     LLARPADQGLIDRDQSLLVIHEKVIEKLLTAALPFEQRVAGQIIVRLEAVAVDFDDGV
                     PILHLEGTAAPTVAKGKPPHPATLTVAVEGVIESIVFEPERGILRARARVLAVESHRT
                     GVKADGWRGFVSEVVRLQSRSFEGVNYDIEIPIRVIDRITLPDLANDDKPSLPVTIEP
                     ASIPLDVSVGEPTALFDRLWIPIHLLSGVRLDSTSARTGPVHEMRPGRPKSRRGKAPR
                     PPLPTDLESTLRDSLDRAVRADPVLVAIMGKEDQVTVATSQRLLNALIEQVADDYLDR
                     VDVMMDADIVEDETGALAVGTPVGRVTAGKWSIWLRVETLRGRLKAGRPRVQVGREGE
                     VAIRVPTRIETGGGRLTLDFAWKPKKLVSILCRGFRDTVSVTAKILPQEHELVGTVRF
                     TDGPEGLRLDTTIRRDRHPIAMTLSDSSWAAVRASLAEQDKFSRCGMLLDPDDALEKL
                     KSLGAAGIRIRMPERLFPSVTLPTRVAKSVLILDKPVALVAGPNKAGSKGGVIWSSAD
                     IGVLPTTDIIPPESRKEGGSEPTGNSVPPNRPTQ"
     gene            complement(6047..7018)
                     /locus_tag="VF720_15520"
     CDS             complement(6047..7018)
                     /locus_tag="VF720_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880821.1"
                     /translation="MSRNLDTVLLTALPASGKSEVRRWLAHQDPAVQAREYHMGETVQ
                     LDDYPYVHMMRRISEELARAGRDPIFFPSGMEPFKDSLDWGTLIELINEDYEDLHAKP
                     AIKPASAAAWLFDRMDEARKKVGADPALGKLPADVRKSLETTLEAEARQLLDDKLAGI
                     PASLEGKTIIIEFARGGPDGSSMPLESPYGYQYSFSKLSDHILGRASILYIWVTPEES
                     RRKNEERARPGRDGDASILHHGVPIKVMLGDYGTDDMAHLIETSDKPGTVTVRTRGKS
                     FHLPVTRFDNRNDLTTFIRDDPSKWPAAQDNAIRAEMKRALGVIAPA"
     gene            complement(7046..>7676)
                     /locus_tag="VF720_15525"
     CDS             complement(7046..>7676)
                     /locus_tag="VF720_15525"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002647794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7880822.1"
                     /translation="PASQPAKPKTMGIDKSMLMDPSKLTEQAPATYKVDFVTSQGNFQ
                     VTVNRAWSPNGADRFYNLVKNGFYDDVRFFRVLENFMAQFGINGDPAVQSHWREATLK
                     DDAPTGANKQSNKRGFLTYAKTGMPDSRSTQLFINFKDNSMLDPQGFTPFGQVTTGME
                     VVDKLYKGYGEGAPRGQGPDQGRLQAEGNAYLNKEFPKLDYIKTARLAK"
BASE COUNT         1208 a         2429 c         2731 g         1308 t
ORIGIN      
        1 ggccgaggcc atgcgcaacg cgctcgacgc cgcgccggcc aatccctcga gcgtccacgc
       61 ggccggcaag gccgcacgcc gcctcgtgga tggcgcccgc cgttcggtgg ccggcctggt
      121 cggcggacgc cccgaggaga tcatcttcac cggatccggg agcgaggccg cctggatcgc
      181 gctgcacggc gcccttgagg ccgcccgcga cgaggacgcg ccaccactgg tcgtatcgtc
      241 gcccatcgag catgcggccc ttcgtgagcc cctggcggcc ctggacgaag ccggcgtcat
      301 ccgcctcacg ctggcaccca tcggtgccac cgggctggtc gacgccgact gcttcgagga
      361 aatcctcgcc gatcgtcccg cgctggtcgt ctgccagtgg gccaacaacg agacgggcgt
      421 gatccagccg gtcgatatca tcggcaccgc ctgccgcgcg gccggcgtgc cattcctggt
      481 ggatgccgtg gccgcagcgg gacgtctcga tctcagggcg acccacggca tcgccgacct
      541 cgtcgtcctt tcctcggcca agctcggcgg ccccgccggc gtcggtgccc tctggatccg
      601 tcccggactc tcgctcactt cgcctctcgg cgccggcggc caggagcagg gcctccgtgg
      661 cggcaccgaa aatgtcatcg gtatcgtggg attcggagcc gccgccgcgg cggcggcgac
      721 aaccgatgtc ggcgggcttg ccgcggtcat ggcgcatggg gtgagtcgtg tgcgtgagcg
      781 cgtgtccgga gccctcctcg cgggagatgg ctcccctcgc ctcgccaaca ccgcccagtt
      841 cgtcgtgccg cacgatgacg aggagatgct gatcctggcc ctcgaccgtt tcggttacgc
      901 cgtgtcggcc ggctcggcct gcgccgccgg cgcccacaag cgttcgcatg tgctcgaggc
      961 catgggcctc ctgggcccgg ggctggcgtc ggttcgcgtc tccatcggcg ccgacacgac
     1021 gaagggcgac atggacgact ttgccgaggc gctccgcgcc tgcgtccgcg cggccgcgcc
     1081 ggccgaggcc gaggagattg tcgagtggcc caccgggggt gccggatgag caagggactc
     1141 gtggtcagcg ccatgtccgg cggggtcgac agcgccgtgg ccgccgccct gctccgcgag
     1201 caggggtacg acgtgtacgg cgtcacgatg aagctctggt gctaccagcg cgaggcgccg
     1261 acggccaggg cctgttgttc gatggcggcg atcaccgagg cccgttccac ggccgatctc
     1321 ctcggcatcg agcacgcggt cgtcgacctc gaggagcagt tcgaagcgca cgtcattggt
     1381 ccgtttgccg aggactacca gaacggccga acgccgaacc cctgcgtgaa ctgcaacaca
     1441 ttcatcaagt tcggcacgct tctcgagaag gcacggcgcc ttggcgccgg ctgggtggct
     1501 accggtcact acgccctgat gagccatgag gagtccggcc cggtgatgcg tcgcggcgtg
     1561 gatgattcga aggaccagtc gtacgccctc tggggcctca accggaagcg cctcaaccgc
     1621 gtgttgatgc cgctcggagc cctgaccaag accgaagtgc gcgaacgggc ccgcgccctg
     1681 gcactgccgg tggccgacaa gcccgagagc atggacatct gcttcgtgga aggtgggcac
     1741 tacgccgact tcctcacgaa ccggcttggc gcctttgcgc aggccgagcc cgggcccatg
     1801 gtcgatacca gcggcaaggt cgtcggcacc caccagggag tgatgcgcta cacggtgggg
     1861 cagcggaagg ggctgggcct ggcccgcccc gaacccacgt tcgtggtggg gatcgacgcc
     1921 gccacgaaca cgctgaccgt gggggaggag tcgggcctct atcgtcggtc gttcgaggcc
     1981 gggcgtccca acttcgtttc gatggagcac cccacgacgc cgttccgcgc ggaaatccgc
     2041 gtgcgccatc ggggccgtac gatcccgggt cttgtcacgc ccctcgctcc gggacgcctg
     2101 cgcatcgacc tcgaccagga cgagcgcgcc atcacgcccg gccagtcggc cgtgttctac
     2161 gacggggatg tgctgctcgg cggggcgctg atcgagcggg cgatcgactg aggggaaccg
     2221 tcaggcaaac tggcggagag ggtgggattc gaacccacgt acgggtttcc ccgtaaacac
     2281 cttagcaggg tgccaccttg agccactcgg tcacctctcc gcctcaaggc aaggccggaa
     2341 aggctaccag agggggacgg caggaacaac caatcaccgg cgggacctgg tcggccgccc
     2401 cgcgttgtcg ggcccctggc aattctcctc ggcggggatg gtggcattgc agccggggga
     2461 agccacgcgg cggtattcgg tgcggcccca ctcgcgcgtc aggagattgc cattctccgt
     2521 gcattcgaag cggtgcacgt cgatgaacca gcacgactcc acgcggacgg tggtatcgct
     2581 ggccgaggcc caggtggtgt tccagcaggt gtcgccgaag acgaagtagc cggtgccgtc
     2641 ctcgaagtag cagcggaagt cgacgcgggc gtcatgattc gtggcctccc agcagccgac
     2701 gaggcaggga ggcggggcgg ggtcgtcctc gtctgcggag cagccggcaa cggccaggaa
     2761 ggccgccgcg agcaggaacg atcgggtcat cgtgagtcgg ccccgggcca gaaaaggctc
     2821 ggggtggccg ccttgggtcg accaccccga atctccggaa actgcggctt ctagaacatg
     2881 attccgaaat tgagaccgaa gccgtcaaag tcggagaaga cgtacttggc ctcgaagaag
     2941 aggctcttcg cggagtcgcc aagcggggtg gaaaggccgg cgccggcgtt gatgccgaac
     3001 tccgtgttgt cgaagtcgga gtcggcgatg aggtccacgc cgcccaggcc gtagaagttc
     3061 atcttgccgg agttcttgaa gtcgagatgg agatcgacgt cggcatcgaa gaactcgtcg
     3121 tcaaagaagt agccgaactg cgggacgatc atgagcttct tgccggcggc cgtcttgaag
     3181 cgtccctgga taccgaaatc ggcatcttcg cggaacatgc cgccaatgcc gaagcccgcg
     3241 tgagcggtcg agaacatccc ggcgccgagc gccatcatgg tgacgaggcc ggcgacggtg
     3301 aacacgcgaa ggcccgccct gcgaatcgat atcatcggta tttcctccct cgggggttga
     3361 tggcgccgcc gcaatcgcga ccgggcactg gatgccaatg aacctagaca accggaagcg
     3421 tggccgcaag tggtggcggg atcaaggaat ggcgggatgg ggcggtcccc gattcagggg
     3481 atctcgatgc tggttgccgt cgggccaaga tggacgggag tccggtcgtc ctggtgcacc
     3541 cagtagtcga ggccggtgcg ggcatggaag agcaccttgt gcgtcaccgg tgacggccag
     3601 atggagaagg tccggttcag catctcgggc tcggtgtcgc ggctgatgtt caggcagatg
     3661 gggcagccga tcgtcacggc gtaggcggcc atcgagtcgc cgcaggccgt ggccggccag
     3721 atgtgcacga gggccgttcc tggcacctgg atgtcgatga ggcgcgcgcc gtcgccggcg
     3781 taatcggtcg ggtggtaggc ctgcacgctg gcctcgtagg ccgtcgggta ttcctcgcag
     3841 aggcatggcg cgctgttgca gtcggtaacc gacacgggga ttccggggcg aatgccctgg
     3901 atgagccgca ccggtgcgct gggcggggtg tcctccccgc atccggcggc gatcggcagc
     3961 agagccacga tccagaacag gcttgatgtc acgcttcgcg cggacacgca ctctcctcaa
     4021 gacagggaag ccccggcgtg cggagatgtg gggacgagag gtacgtcggt tccgcctgcc
     4081 gtgatcatga caaggagtgc aagacgcgga ccacgcaata cgtggcatgg atggccattc
     4141 aagggtctgc ccggttgata cgtgggggat ggcctgacga atttactgag tcgggcgatt
     4201 cggcgggacg gagttaccgg tgggttccga acccccctct ttgcgcgatt cgggtggaat
     4261 gatgtccgtg gtggggagaa ccccgatgtc cgcactggac cagatcactc ctcccttgct
     4321 cccggccttg ttgggcccgg ccaccagcgc gaccggcttg tcgaggatca ataccgactt
     4381 ggccacccgg gtggggagtg tcaccgacgg gaagagacgt tcgggcatcc ggatgcggat
     4441 gccggcggcg ccgagagact tgagtttttc gagtgcgtcg tccgggtcga ggagcatgcc
     4501 gcagcgggag aacttgtcct gctcggcgag cgaggcgcgc acggccgccc aggacgagtc
     4561 ggagagcgtc atcgcgatcg gatgtcgatc ccggcggatg gtggtgtcga gcctgagccc
     4621 ctccgggccg tcggtgaacc ggaccgtgcc gaccagctcg tgctcctgcg gcaggatctt
     4681 cgccgtcacg ctcaccgtgt cgcggaagcc gcggcagagg atcgacacca gcttcttcgg
     4741 cttccaggca aagtcgagcg tgaggcgacc gccgcccgtt tcgatgcggg tggggacgcg
     4801 gatcgccacc tctccctcgc gccccacctg gacccgcggg cgcccggcct tgaggcgacc
     4861 gcgaagcgtc tcgacgcgca gccagatcga ccacttgccg gccgtgacgc ggccgaccgg
     4921 cgtgccgacg gcgagggcgc cggtttcgtc ctccacgatg tcggcatcca tcatcacgtc
     4981 cacccggtcg agatagtcgt cggccacctg ttcgatcagg gcattcagca accgctggct
     5041 ggtggccacc gtgacctggt cttccttccc catgatggcc acgaggaccg gatcggcgcg
     5101 aaccgcacgg tcgaggctgt cgcgcagcgt cgactcgagg tcggtcggta gcggtgggcg
     5161 aggcgctttt ccgcgccggg atttcggccg accgggacgc atctcatgga ccggtcccgt
     5221 gcgggcgctg gtcgagtcga gtcggacgcc ggaaaggagg tgtatgggaa tccagagccg
     5281 gtcgaagagg gcggttggtt ccccgaccga cacgtcgagc gggatggagg ccggctcgat
     5341 cgtgaccgga aggctgggct tgtcatcgtt ggcgaggtcc ggaagcgtga tccgatcgat
     5401 gacccggatc gggatctcga tgtcgtagtt caccccttcg aaactgcgcg actggagtcg
     5461 cacgacctcg ctcacgaagc cgcgccaccc gtccgccttg accccggtgc ggtgcgattc
     5521 gacggccagc acgcgggcgc gggcgcgcag gattccccgc tcgggttcga agacgatcga
     5581 ctcgatgacc ccttcgaccg ccacggtgag cgtcgccgga tggggcggct tgcctttcgc
     5641 caccgtcggc gcggccgtcc cctcgaggtg aaggatcggg acgccatcgt cgaaatcgac
     5701 cgccaccgcc tcgagccgga cgatgatctg cccggctacc cgttgttcga aggggagggc
     5761 cgcggtcagg agcttctcga tgaccttttc gtggatcacg agcagcgact ggtcgcggtc
     5821 gatgagtccc tggtcagccg ggcgggccag gagcgtctgc atggcggtca cgcgtcgatc
     5881 ggagaggaca gtctcgcgcg ggaggtcctt gcggaaggaa tggcatcctg cgagtagcgg
     5941 gacggcaaga agcgcgacca gcaacgatgt cgcgcgccag gtcatggcag gaggcgccga
     6001 cggatcccgg ggaagaccgt catcgaatgc gtcgtcgccg gcctgcctac gcgggcgcga
     6061 tcactcccag cgcccgcttc atctcggcgc ggatggcgtt gtcctgcgcg gccggccact
     6121 tcgacggatc gtcgcggatg aacgtggtca ggtcattccg gttgtcgaaa cgggtgaccg
     6181 ggaggtggaa cgacttgccg cgggttctga ccgtgacggt gccgggcttg tcgctcgtct
     6241 cgatcaggtg ggccatgtcg tcggtgccgt agtcgcccag catcaccttg atgggcacgc
     6301 cgtggtggag gatggaggcg tcgccgtcac ggcctggcct ggcgcgctcc tcgttcttgc
     6361 ggcgcgactc ctcgggcgtc acccagatgt agaggatgct ggcgcggccg aggatgtgat
     6421 ccgacagctt cgagaagctg tactggtagc cgtaaggcga ttcgagcggc atcgatgagc
     6481 cgtcggggcc gccgcgggcg aattcgatga tgatggtctt cccctcgagc gaggcgggga
     6541 tgccggccag cttgtcgtcg aggagctggc gcgcttcggc ttcgagcgtc gtctcgagcg
     6601 atttccggac gtcggcgggg agcttgccga gcgcggggtc ggcgccgact ttcttccggg
     6661 cctcatccat ccggtcgaac agccaggccg cggccgacgc cggcttgatg gccggcttcg
     6721 cgtgcaggtc ttcgtagtct tcgttgatca attcgatcag cgttccccag tcgagggaat
     6781 ccttgaaggg ctccatcccg ctggggaaga agatcggatc gcggccggcc ctggcgagct
     6841 cctcggagat ccgccgcatc atgtggacgt aggggtaatc gtcgagctgg accgtctcgc
     6901 ccatgtggta ttcgcgggcc tggacggcgg ggtcctggtg agccagccac cgccggacct
     6961 cggatttccc cgaggccggc agggcggtca ggaggacggt atcaagattg cgactcatga
     7021 acgtcctcct gagggaaggg ttggtttact tcgcgaggcg ggcggtcttg atgtagtcga
     7081 gcttcgggaa ttccttgttc agataggcgt tcccttcggc ctggaggcgt ccctggtcgg
     7141 gaccctggcc gcgcggcgcg ccttcgccgt agcccttgta gagcttgtcg accacttcca
     7201 tgccggtggt gacctgtccg aacggcgtga agccctgcgg atcgagcatg gagttgtcct
     7261 tgaagttgat gaagagctgg gtggagcggc tgtccggcat gccggtcttg gcgtaggtga
     7321 ggaagccgcg cttgttgctc tgcttgttgg ccccggtcgg cgcgtcatcc ttgagcgtgg
     7381 cttcacgcca gtgactctgg acggccggat cgccgttgat gccgaactgg gccatgaagt
     7441 tctcgagcac acggaagaag cggacgtcgt cgtaaaaccc gttcttcacg aggttgtaga
     7501 aacggtcggc gccgttgggc gaccaggcgc ggttgaccgt cacctggaaa tttccctggc
     7561 tggtgacgaa atcgaccttg taggtggccg gggcctgctc ggtgagcttg gaggggtcca
     7621 tcagcatgct cttgtcgatc cccatggttt tcggcttggc cggctgcgag gccggc
//
LOCUS       DASVOD010000720         2594 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_308571, whole genome shotgun sequence.
ACCESSION   DASVOD010000720 DASVOD010000000
VERSION     DASVOD010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308571"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VF720_15530"
     CDS             <1..338
                     /locus_tag="VF720_15530"
                     /inference="COORDINATES: protein motif:HMM:NF012887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031072 - heat shock protein binding
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HEX7880823.1"
                     /translation="APGHAPVTCPTCAGRGTVVQNRGGFSVSRTCPTCYGRGQVIDKP
                     CPACGGEGMRFDERKLRVKIPAGAKPGQKLRLRGQGHAGTAGEPAGDLIVTLNVMADA
                     ANDAKKRGA"
     gene            370..2181
                     /locus_tag="VF720_15535"
     CDS             370..2181
                     /locus_tag="VF720_15535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880824.1"
                     /translation="MSPGRRPFPASWPVVPAYLILSIVLTWPVARDFGHSIPAVFGAL
                     DPLLQSFLLGWDWQALATAPSRIFHLPIFHPHERALTYMDHLIGEALLAWPVRLLTGR
                     TAPAYNALVLGSFVFSGWFMYRLCRGIGISRRGSFLGGLAFAFGPYRFANLGNLNQLQ
                     TQLLPLGLHLALRCHRGQRLTDMAGLLAVLVIQSTLGWYYFFHLALVTALVFGWEALR
                     GRCGWRKGALPRVVLMGTAAVALVLPLLRPYLEQQRAMPAFRRTIGMTALWSADVVDY
                     LRVTVESAWAGLWWNGAQGYWPGFLAVPLALFGVWGVVASRSASSIVAGRSRESGSSW
                     YSMRVAAWLGERARSWGDAGHLLLIGLFGWILSLGPVLHVAGTRTPVPLPFAAAFFVV
                     PGFSSMRAPSRFAVLVLLAASVLAAIGWDRLMRRPAMQSDDRRLFALLVALFIGVAGA
                     WPSTIPMTPAPHRTEMPPVYAWLAAQPDRLPLAELPMPEQVTDEDPTHARRQAWLLEH
                     GHPRLDGVSGFVPPDDETLRVVMQGFPRAGPLNALDRRGTHWIIVHYGDYPAGQAAWI
                     EAEAAAGGRLEEAARFGTDVIYRLKPAGSATGSQFNR"
     gene            complement(2130..>2594)
                     /locus_tag="VF720_15540"
     CDS             complement(2130..>2594)
                     /locus_tag="VF720_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880825.1"
                     /translation="LHPAPPNLHAAIPREGPGAVATWLDRSGESDTVAEFPMPPDDSR
                     GDVVRARRQLASIYHWRRLVDGQARRTPPLARLVRHRLAGFPDQVSMAQLRALSIDLA
                     VVHVDEYEPDEWLAVSDRFKEWPELELEQDLGSARVYRLNWEPVALPAGLSR"
BASE COUNT          309 a          929 c          913 g          443 t
ORIGIN      
        1 ccgcgcccgg gcatgcgccc gtgacgtgcc ccacctgcgc cggccgcggc accgtggtgc
       61 agaaccgggg cggcttctcg gtcagtcgca cctgccccac ctgctacggc cgcgggcagg
      121 tcatcgacaa gccctgcccg gcctgcggcg gcgaggggat gcgcttcgac gagcgcaagc
      181 tccgcgtgaa gatccccgcc ggcgcgaagc ccggccagaa gctgcggctg cgcggccagg
      241 ggcatgcggg cacggccggt gaacccgccg gcgacctgat cgtgacgttg aacgtgatgg
      301 ccgacgcggc gaacgacgcg aagaagcgcg gggcttgatc gccggccggc cgcggtcgac
      361 gatgcgcgca tgagccccgg ccgccgaccg tttcccgcat cctggccggt cgtgccggct
      421 tatctcatcc tgtcgatcgt cctcacctgg ccggtggcgc gcgacttcgg ccactccatt
      481 cccgccgtgt tcggggcact cgacccgctc ctccagagct tcctcctggg ttgggactgg
      541 caggcgctgg ccaccgcgcc gtcgcggatt tttcaccttc cgatcttcca tccgcacgag
      601 cgcgcgctca cctacatgga tcacctgatc ggtgaggcgc tcctggcctg gccggtccgc
      661 ctcctcaccg gacggacggc gccggcctac aacgccctcg tgctcggctc gttcgttttc
      721 tccggctggt tcatgtaccg gctctgccgc gggatcggca tctcgcgccg ggggtcattc
      781 ctgggcggcc tggcctttgc cttcggcccg taccgcttcg ccaacctcgg gaacctgaac
      841 cagctccaga cgcagcttct tccgctcggc ctccacctgg ccttgcgctg ccaccgcggg
      901 cagcgcctca cggacatggc cggccttctc gccgtcctcg tcatccagtc gaccctgggc
      961 tggtattact tcttccacct ggcgctggtc acggcgctcg tcttcggctg ggaggccctg
     1021 cgcgggcgat gcggctggcg aaagggggcg ctgccgcgcg tggtcctgat gggaacggct
     1081 gccgtcgcgc tcgtcctgcc gctgctgcgg ccctacctcg agcagcagcg ggcgatgccg
     1141 gccttccggc gcacgatcgg catgacggca ctctggtcgg ccgatgtcgt cgactacctc
     1201 cgcgtgacgg tggagagcgc ctgggcgggc ctctggtgga acggcgcgca gggatactgg
     1261 cctggtttcc tggcggttcc gttggcgctc ttcggtgtat ggggggtggt cgcgtcgcgg
     1321 tcggcttcgt cgatcgtcgc cggccggtcc cgtgaatccg gatcgagctg gtactcgatg
     1381 cgcgtggccg cctggctcgg cgagcgtgcc cgttcctggg gcgacgccgg gcatctcctc
     1441 ctcatcggcc ttttcggatg gatcctctcg cttggcccgg tgctccacgt ggccggcacg
     1501 cggacgcccg tgcccctgcc gttcgccgcg gccttcttcg tcgttcccgg cttcagcagc
     1561 atgcgtgcgc cgagccggtt cgcggtgctg gtgctgctcg cggcctcggt cctcgcggcg
     1621 atcgggtggg accggttgat gcgccggccg gccatgcaat cagacgaccg gcgtcttttc
     1681 gcactcctgg tggcactttt catcggcgtg gcgggtgcgt ggccctccac catcccgatg
     1741 acgccggcgc ctcatcgcac cgagatgccg ccggtttacg cctggctggc ggcgcaaccc
     1801 gatcgactgc cactggccga gttgccgatg ccggaacagg tgaccgatga ggacccgacg
     1861 cacgcgcggc ggcaggcctg gctcctcgag cacggccatc cgcggctcga tggcgtgagc
     1921 ggcttcgtgc ccccggacga cgagacgttg agagtcgtca tgcagggctt cccgcgggcg
     1981 ggaccgttga acgcgctcga tcggcgcggc acgcactgga tcatcgtcca ctacggcgat
     2041 taccccgccg ggcaggcggc ctggatcgag gcggaagcgg cggccggcgg acggctcgag
     2101 gaggccgccc gcttcggaac cgacgtgatc tatcggctca agcccgcggg gagtgccacc
     2161 ggctcccagt tcaaccggta aacgcgggcc gagccgaggt cctgctccag ctcgagctcg
     2221 ggccactcct tgaagcggtc ggagactgcc agccactcgt ccggttcgta ctcgtcgacg
     2281 tggaccaccg ctaggtcgat cgagagggcg cggagttgcg ccatcgacac ctggtcgggg
     2341 aatcccgcca ggcgatgccg caccagacgg gccagtggag gggtgcgacg ggcctggccg
     2401 tcgacgagcc gccgccagtg atagatggag gcgagctgcc gccgtgcgcg gaccacgtcg
     2461 cctcgtgagt cgtcgggtgg catcgggaat tcggccacgg tgtcggactc gccgctccgg
     2521 tcgagccagg tggccacggc ccccggccct tcgcgcggga tggccgcgtg caggttgggc
     2581 ggcgccgggt gcag
//
LOCUS       DASVOD010000721         8920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_308592, whole genome shotgun sequence.
ACCESSION   DASVOD010000721 DASVOD010000000
VERSION     DASVOD010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308592"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /locus_tag="VF720_15545"
     CDS             <1..2006
                     /locus_tag="VF720_15545"
                     /inference="COORDINATES: protein motif:HMM:NF023559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEX7880826.1"
                     /translation="PHQLHVAELAFRADPVRWLLADQVGLGKTIEACLILSRLLRTSR
                     AERVLVVAPSTLTVQWLGELYRKFHQIFVLLDAERRQDVRKDHPGFNALEIHPRSVIA
                     LEDLVADTQVGREAIDAKLDLLVVDEAHRLERRPGHPGNPAYRAIAPLAAASRNVLML
                     SATPLEADAHGFYRLLELLWPNEFPSWDKFEADLAAGVPIYPCTSSTTRDDIGGLPPR
                     VAEAAELGPWPDLEVAEAGAMAMPVSNVLEKKRRLEAFQAALHEPHGTEDPRLLWIVE
                     QAARWKKRGEKNLIFVSNRPALDFLKREIEFRLGTRVAVFHEDLSPGQRDIEVAQFAQ
                     SDGAGLLISTECGGEGRNFEFCRRLVLFDLPWNPALVEQRIGRLDRISRKRPVEIAYF
                     KPMTGFARQLVELYERLGMFNQPLGGLERSLTHVEEAIEAAALGEVPLDIDAVIRETL
                     EARERMTKAVYHHLHQNRYQPELAKPIIARIPPELETLTEQVVVSACQQFGFDVIDRP
                     EQNTWYVEFGGTAVIDHLPGATSGSRWLGTFDRKNAVARESIDFFASGHPLVEGILME
                     LEDGHRGQIALLEAADTGVDAIGLIVVLKRGSEFDIHILDADGQPHPEWYPFLLETGA
                     KVRDMHPGHWSPPEGFDDWVRSVVQVVRETGKPMALAGFRLGA"
     gene            2325..2717
                     /locus_tag="VF720_15550"
     CDS             2325..2717
                     /locus_tag="VF720_15550"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX7880827.1"
                     /translation="MIYLDSSVALAQIQAESRRPSPGFWDNELVASRLLQYETWTRLH
                     AAGRGQSHGEAARILLAGIMFIELTPEILEPLVEGFRLPLKTLDAIHLATLRWLDEQQ
                     TRVQLATYDEKLASTARRLGFELHPVSG"
     gene            complement(2819..3286)
                     /locus_tag="VF720_15555"
     CDS             complement(2819..3286)
                     /locus_tag="VF720_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HEX7880828.1"
                     /translation="MNDPTRMPAGPARPLNATPTTVTDASTTVPAVDTPAADANVEAA
                     TPGAPLDSAARKALEERVVALLKEVYDPEIPVNIYDLGMIYDIQINEAAAAHVTMTLT
                     SPMCPVAGTLPGEVETKIRSLPGVSAASVELTWEPPWDKEMISEAGKLVLNIF"
     gene            complement(3291..3740)
                     /locus_tag="VF720_15560"
     CDS             complement(3291..3740)
                     /locus_tag="VF720_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX7880829.1"
                     /translation="MNDLRELYQEIILDHYKKPRNFHAMPDATAHADGYNPLCGDKVK
                     VHLKVADGVVTDVAFEGSGCAISTASASIMTDALKGKSIDEARNLFETFHEMLTSPEP
                     TVDPATLGKLAVFGGVREFPVRVKCATLAWHTMKAALDNSNDPVSTE"
     gene            complement(3737..4993)
                     /locus_tag="VF720_15565"
     CDS             complement(3737..4993)
                     /locus_tag="VF720_15565"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX7880830.1"
                     /translation="MSTAHATRSAESRVFDVAAIRREFPILSRTVNGRPLVYLDNAAT
                     SQKPRAVIDTLSGYYEEINANIHRGVHWLSQRATDRYEAARESARRFINAGSHEEIVF
                     VRGTTEAINLVAQTYGRTHIHEGDEIVISHLEHHSNIVPWQLLAAERKAILRVVPIND
                     AGEFQFEAYASLLCDRTKLVAVSHVSNALGTINPVREIIALARSRGIPVLLDGAQAVP
                     HIAVDVRDLGADFYTFSGHKMYGPTGIGVLYARSEWLESLPPWQGGGDMILSVSFDGT
                     TYNKPPFKFEAGTPNIEGGIGLGAAIEWLTNIGLDNVAAHEHELLVYGTKCLQRIEGL
                     KLVGTARQKAGVLSFTLDGIHPHDIGTILDQAGIAIRTGHHCAQPVMDRFNIPATARA
                     SLAVYNTTEDIDALVEGIDQVVRMFR"
     gene            complement(4995..6335)
                     /gene="sufD"
                     /locus_tag="VF720_15570"
     CDS             complement(4995..6335)
                     /gene="sufD"
                     /locus_tag="VF720_15570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX7880831.1"
                     /translation="MTLDVTLDRDHFVVRHGELAKSAGTPAWLASLRQKAISHYQGIG
                     LPTTHQEEWRFTNTQTLNGTRFEPAPAATAAGIDIAAIERLTLGGDLFRIGLVNGRFS
                     PELTNLAGLPAGVTVQTLSDALRASSPIVEANLGRHARWENNAYSALNTALFEEGVVV
                     HVGKGVVLEKPIHVLHVTIPGSGSPGVTHLRNLIVAEESAQVRVIESYVGRRGTYWTN
                     AITEIVAGDNAVADHYRLNRESDGAFHTGRVDMFAGRSGVTNTHTITFGGLLTRNDTG
                     VVLGGEGADSTMNGLTVIGGKQLVDNHTSIDHAVPHCTSHELYKAVLDGTARTVFNGR
                     ILVRKDAQKTDSKQTNKNLLLSDKALVNTNPQLEIYADDVKCTHGATIGQLDESMIFY
                     LRSRGIGEVAAQHLLTYAFANDIVERIQVPALRERLNDYLIHRLRDEDGAEEAL"
     gene            complement(6346..7101)
                     /gene="sufC"
                     /locus_tag="VF720_15575"
     CDS             complement(6346..7101)
                     /gene="sufC"
                     /locus_tag="VF720_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX7880832.1"
                     /translation="MLDIKNLSASVGGKTILKGINLTLKPGEVHAIMGPNGSGKSTLA
                     QVLAGRENYEIEGGSVDFLGKDLLDMSPEDRACEGVFLAFQYPVEIPGVSNSYFLRSA
                     LNAIRKYHGQPEVDAMDFLAMVKEKAKLVRMDEGLINRAVNEGFSGGEKKRNEIFQMA
                     VLDPALAILDETDSGLDIDALRIVADGVNALRKQDRAILVITHYQRLLDYIVPDYVHV
                     LADGRIIKSGDKTLATELEAKGYGWLDDLVAAR"
     gene            complement(7134..8582)
                     /gene="sufB"
                     /locus_tag="VF720_15580"
     CDS             complement(7134..8582)
                     /gene="sufB"
                     /locus_tag="VF720_15580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX7880833.1"
                     /translation="MTTANQTIEELANREYKYGFTTDIEADQFPPGLNEDVVRALSAK
                     KGEPEWLTEWRVKAYKHWTTMSDPEWANVHYPPIDFNAISYYSAPRQKTDGPKSLDEV
                     DPKLLETYEKLGIPLAERALLAGVAVDAVFDSVSVATTFKDKLSEVGIIFCSFSEAVK
                     EHPELVKKYLGSVVPYTDNYFGALNSAVFSDGSFVYIPKGVRCPMELSTYFRINASKT
                     GQFERTLIIADEGATVSYLEGCTAPMRDENQLHAAIVELIAHDNATIKYSTIQNWYPG
                     DHEGKGGIYNFVTKRGKCQGKNSKISWTQVETGSAITWKYPSCILQGDNSVGEFYSVA
                     LTNHYQQADTGTKMIHIGKNTRSTIVSKGISAGFGQNTYRGQVKILAGAEGARNYSQC
                     DSLLLGDKCGAHTFPYIEVKNPTAKMEHEASTSKIGEDQIFYCKARGLSTEDAVSMIV
                     NGFCKEVFRELPMEFAVEAQKLLSVSLEGSVG"
     gene            complement(8579..>8920)
                     /locus_tag="VF720_15585"
     CDS             complement(8579..>8920)
                     /locus_tag="VF720_15585"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX7880834.1"
                     /translation="LPMVGKILKALVHRGLLISHRGVKGGYQLAGKPEGISVATIIEA
                     IDGPIGLTTCSVHSGECAHEGRCPSRPNWNRINAAVLQSLRGISLADMARPMSFPNLT
                     PAAAPLAGEIA"
BASE COUNT         1448 a         2823 c         2981 g         1668 t
ORIGIN      
        1 tcccgcatca gctgcacgtg gcggagctcg ccttccgggc cgacccggtc cgctggctgc
       61 tggccgacca ggtgggcctt ggaaagacga tcgaagcctg cctcatcctc tcgcgcctgc
      121 tccggacaag tcgcgcggag cgcgtcctcg tggtggcgcc gtccacgctc accgtgcagt
      181 ggctcggcga gctctatcgg aagtttcacc agatcttcgt gctgctggat gccgagcgac
      241 gccaggatgt gcgcaaggac catcccggct tcaacgcgct ggaaatccac ccgcgctccg
      301 tcatcgcgct ggaagacctg gtggcggata cgcaggtcgg ccgcgaggcg atcgacgcga
      361 agctcgacct cctggtcgtg gacgaggcgc atcgactcga acgtcgcccc ggccatcccg
      421 gcaacccggc ctaccgcgcc atcgcgccgc tggccgccgc cagccgcaac gtgctgatgc
      481 tctcggccac accgctcgag gccgacgcgc acggcttcta tcgcctgctc gagctgctct
      541 ggccgaacga attcccctcg tgggacaagt tcgaagccga cctcgccgcc ggcgtgccga
      601 tctacccctg caccagttcc accacgcgcg acgatatcgg cggcctgccg ccccgtgtag
      661 ccgaagcggc ggaactcggc ccctggcccg acctcgaggt ggccgaggcc ggcgccatgg
      721 cgatgcccgt ctccaacgtc ctcgagaaga agcggcgcct cgaagccttc caggccgcac
      781 tgcacgaacc gcacggcacc gaagacccgc gcctcctctg gatcgtcgag caggccgccc
      841 gctggaagaa gcggggggag aagaacctca tcttcgtgtc gaaccgcccc gctcttgatt
      901 ttctgaagcg cgaaatcgaa ttccgcctcg gcacgcgcgt ggcggtgttc cacgaagacc
      961 tttcgccggg ccagcgcgac atcgaggtgg cgcagttcgc gcaatccgac ggggccgggc
     1021 tcctcatctc caccgagtgc ggcggcgaag gacgcaactt cgaattctgc cgccgactgg
     1081 tgctgttcga cctgccgtgg aacccagcgc tggtggaaca gcgcatcggc cggctcgatc
     1141 gcatcagccg caagcgtccg gtggagattg cgtatttcaa gccgatgacg ggcttcgccc
     1201 gacaactggt ggagctctac gagcgtctcg gcatgttcaa ccagccgctg ggcggcctcg
     1261 agaggtcact cacgcatgtc gaggaggcca tcgaagccgc ggccctcggc gaggtgccgc
     1321 tcgacatcga cgccgtcatc cgcgaaacgc tcgaagcccg cgagcggatg acgaaggccg
     1381 tgtaccacca ccttcatcag aaccgctacc agcccgaact ggcgaagccg atcatcgcgc
     1441 gcatcccgcc cgagctcgaa acgctgacgg aacaggtggt ggtatccgcc tgccagcagt
     1501 tcggcttcga cgtcatcgac cggccggaac agaacacctg gtacgtggag ttcggcggca
     1561 ccgccgtcat cgaccacctc ccgggcgcca cctccggcag tcgctggctc ggcacgttcg
     1621 atcgcaagaa cgccgtggct cgcgagtcga tcgacttctt cgcctcgggt catccactgg
     1681 tcgaggggat cctgatggag ctggaggacg gccaccgggg tcagatcgcg ctgctcgaag
     1741 ccgccgacac cggcgtcgac gccatcggcc tcatcgtcgt gctcaagcga ggatcggaat
     1801 tcgacatcca catcctcgac gccgatgggc agccgcaccc cgagtggtac ccgtttctgt
     1861 tggaaaccgg cgcgaaggtc cgggacatgc accccgggca ctggtcaccg ccggagggtt
     1921 tcgatgactg ggtgcggagt gtggtgcagg tggtgcggga gacgggcaag ccgatggcgc
     1981 tggcggggtt ccggctgggg gcttgacaaa gtagccactg gtagctactt taggtttatg
     2041 aaagctgtcg ggatcaaagt cctcaagaac aagttgagcg agtatgtgaa gcaggccgcc
     2101 ggtggggaaa tcattctcgt gacggatcgt gaccgggtgg tagcggaatt gggaccgcca
     2161 agtgcggacc ggccccggca ggctggcgag tcgctccttg ctgacgcgat tcggcgtggc
     2221 atcgtaaccc cggctatcaa cccattggct ggtccaccgc ccaacattcc cacagtgccg
     2281 atcgaggaga tcctggatga cctcaggcgt gaccgggacg accggtgatc tatctcgact
     2341 catcggtcgc actggcgcag atccaggcgg agagccgacg tccgtcaccg ggattctggg
     2401 acaacgaact ggttgccagc cgactcttgc agtatgagac ctggacgcgc ctgcatgcag
     2461 cggggcgcgg acagagtcac ggcgaggcag ctcgtattct cctcgcagga atcatgttta
     2521 tcgagctgac gccggagatc ctcgaaccgc tcgtagaagg cttccgtcta cctctcaaga
     2581 cactcgacgc catccacctg gccactttgc gctggctcga tgaacagcaa acgcgtgtcc
     2641 agctggccac ttatgacgag aagctggctt caaccgcacg gaggctcggc ttcgagctgc
     2701 atcccgtctc aggctagaac agtcgagatg gcgaacccac aactcgggcg cccgcggacc
     2761 ggcgcatgac gaagtgactt ctgatcaatc atcgaaattc gagtcgatca ttgacagctc
     2821 agaagatatt caaaaccagc ttccccgcct cggaaatcat ctccttgtcc cagggcggct
     2881 cccaggtcag ttcgaccgaa gccgccgaaa caccgggcag agagcggatc tttgtctcca
     2941 cttcgccggg cagggtgccg gcgacgggac acatcggcga ggtcaatgtc atcgtgacat
     3001 gggccgcggc ggcctcgttg atctggatgt cgtagatcat gccgaggtcg tagatgttga
     3061 cggggatttc ggggtcgtag acctctttca ggagggcgac gactcgctcc tcgagcgcct
     3121 tgcgggcggc gctgtcgagc ggagcgccgg gggtggcggc ctccacgtta gcgtccgcgg
     3181 caggtgtgtc cacggctggc acggtggtgc tcgcgtccgt cacggtcgtc ggagtcgcat
     3241 tcaggggccg ggccgggccg gcgggcatgc gggtcggatc gttcatggat ttactccgtg
     3301 ctcacgggat cgtttgagtt gtccagggcg gccttcatgg tgtgccaggc cagcgtcgca
     3361 cacttcacgc gcaccgggaa ttcgcggacg ccgccgaaga cggccagctt gccgagcgtg
     3421 gcgggatcga ccgtcggttc ggggctggtc agcatctcgt ggaacgtctc gaagagattg
     3481 cgggcttcgt cgatcgactt tcccttcagg gcatcggtca tgatcgaggc ggatgccgtg
     3541 gagatggcgc agccggagcc ttcgaaggcc acgtcggtca ccacgccgtc ggccaccttg
     3601 aggtgcacct tcaccttgtc gccgcagagc gggttgtagc cgtcggcgtg ggcggtggcg
     3661 tcgggcatcg cgtgaaagtt ccgtggcttc ttgtagtgat ccaggatgat ctcctggtag
     3721 agctcgcgca ggtcgttcat cggaacattc tcacgacctg gtcgattcct tccaccagcg
     3781 cgtcgatgtc ctcggtggtg ttgtagacgg ccagcgaggc ccgggcggtg gccgggatgt
     3841 tgaagcggtc catgaccggc tgggcgcagt ggtgtcccgt ccggatggcg atgccggcct
     3901 ggtcgaggat ggtgccgatg tcgtgcgggt ggatgccgtc gagcgtgaac gacagcacgc
     3961 cggccttctg gcgcgcggtg cccaccagct tgagcccctc gatccgttgc aggcacttgg
     4021 tgccgtagac cagcagctcg tgctcgtggg ccgccacatt gtcgagcccg atgttggtca
     4081 gccactcgat ggcggcgccg aggccgatgc ccccctcgat gttgggggtg ccggcctcga
     4141 acttgaaggg cggcttgttg taggtggtgc cgtcgaacga caccgacagg atcatgtcgc
     4201 cgccgccctg ccatgggggg agcgattcga gccactccga acgcgcatac agcacgccga
     4261 tgccggtcgg gccgtacatc ttgtggccgg agaaggtgta gaagtcggcg ccgaggtcac
     4321 gcacgtccac ggcgatgtgg ggcaccgcct gtgcgccgtc gagcagcacc gggatgccgc
     4381 gcgagcgggc cagggcgatg atctcccgga ccgggttgat cgtgccgagc gcgttggaca
     4441 cgtgcgacac cgccaccagc ttcgtccggt cgcacaggag actcgcgtag gcctcgaact
     4501 ggaactcgcc ggcgtcgttg atcggcacca cgcggaggat ggccttgcgc tcggcggcca
     4561 ggagctgcca tggcacgatg ttggagtggt gctccaggtg ggagatgacg atctcgtctc
     4621 cctcgtggat gtgcgtgcgg ccgtaggtct gggccaccag gttgatcgcc tcggtggtgc
     4681 cgcggacgaa gacgatctcc tcgtggctgc cggcgttgat gaagcgccgg gccgattcgc
     4741 gggcggcctc gtaccggtcc gtggcccgct ggctgagcca gtggacgccg cgatggatgt
     4801 tggcgttgat ctcttcgtag tagccgctca gcgtgtcgat gacggcccgt ggcttctgcg
     4861 aggtggcggc gttgtccagg tagaccagcg ggcggccgtt gaccgtccgc gagaggatcg
     4921 gaaactcgcg gcggatggcc gccacgtcga agacgcgtga ttcggccgat cgcgtcgcgt
     4981 gagccgtact caatctagag cgcctcctcc gcgccgtcct cgtcccgcag ccggtggatg
     5041 agatagtcgt tgagccgctc gcgcagggcg ggcacctgga tccgctccac gatgtcgttg
     5101 gcgaaggcgt aggtgagcag gtgctgcgcc gccacttcgc cgatgccgcg cgaacggagg
     5161 tagaagatca tgctttcgtc gagctggccg atcgtggcgc cgtgcgtgca tttcacgtcg
     5221 tcggcgtaga tctcgagctg gggattggtg ttcaccagcg ccttgtccga caggagcagg
     5281 ttcttgttgg tctgcttcga gtcggtcttc tgggcatcct tgcggacgag gatcctgccg
     5341 ttgaacaccg tcctggctgt gccgtcgagc accgccttgt agagctcgtg gctggtgcag
     5401 tgcggcaccg cgtgatcgat cgaagtgtga ttgtccacca gctgcttgcc accgatgacg
     5461 gtcaacccgt tcatcgtgga atcggcccct tcgcctccca gcaccacgcc cgtgtcgttc
     5521 cgcgtgagca ggccgccgaa cgtgatggtg tgcgtattgg tcacgccgct ccggccagcg
     5581 aacatgtcca cgcggccggt atggaaagcc ccgtccgatt cgcggttcag ccggtagtgg
     5641 tcggcaacag cattgtcccc cgccacgatc tcggtgatgg cattggtcca gtaggtgccg
     5701 cgccggccga catacgattc gatgacgcgc acctgggcgg actcctcggc cacgatgagg
     5761 ttgcgcaggt gcgtgactcc gggtgaaccc gaccccggaa tggtgacatg cagcacgtgg
     5821 atcggcttct cgagcaccac gcccttgccc acgtgcacga ccacgccctc ctcgaagagg
     5881 gccgtgttga gcgcggagta ggcgttgttc tcccagcggg cgtggcggcc aaggttggct
     5941 tccacgatcg gggacgacgc gcggagcgcg tccgatagtg tctgtaccgt aacgccggcc
     6001 ggcaggccgg caaggttcgt gagctcgggc gaaaagcgac cgttcacgag accgatgcgg
     6061 aaaagatcac cacccagcgt cagtcgctcg atggcggcga tgtcgattcc ggccgctgtg
     6121 gccgccggag ccggttcgaa tcgcgtgccg ttcagcgtct gcgtattggt gaagcgccac
     6181 tcttcctggt gcgtggtcgg caggcctatg ccctggtagt gggagatagc cttctggcgc
     6241 aggctggcca gccacgcggg cgtgccggcg ctcttggcca gttcgccatg gcggacgaca
     6301 aagtgatccc gatcgagcgt cacatccaac gtcatcggcg gccccttacc tggccgccac
     6361 gaggtcgtcg agccagccat agcccttcgc ctcgagttcc gtggccagcg tcttgtcgcc
     6421 ggacttgatg atgcggccgt cggccagcac gtggacgtaa tcgggaacga tgtaatcgag
     6481 gaggcgctgg tagtgcgtga tgaccaggat ggcccggtcc tgcttccgga gcgcgttgac
     6541 gccgtcggcc acgatcctga gggcgtcgat gtcgaggccg gagtcggtct cgtcgaggat
     6601 ggcgagcgcg ggatcgagca cggccatctg gaagatctcg ttccgcttct tctcgccgcc
     6661 cgagaacccc tcgttcacgg cgcggttgat cagcccttcg tccatgcgga ccagcttggc
     6721 cttttccttg accatggcca ggaagtccat ggcatccacc tcgggctggc cgtggtactt
     6781 cctgatggcg ttgagcgccg agcggaggaa gtacgagttc gacacgcccg ggatctccac
     6841 cggatactgg aaggccagga agacgccctc gcaggcccga tcctccggcg acatgtcgag
     6901 caggtccttg ccgaggaaat cgaccgagcc cccttcgatt tcgtagttct cgcggccggc
     6961 cagcacctgc gccagcgtgc ttttacccga gccgttgggg cccatgatgg cgtgaacctc
     7021 gcccggcttg agggtgaggt tgatcccctt gaggatggtc ttgccgccca cggaggcgga
     7081 gaggttcttg atatcgagca tgatgttggc ttccctttcg aaacggcggc cggtcagccg
     7141 accgagccct cgaggctgac ggacaacagt ttctgggctt ccaccgcgaa ctccatcggg
     7201 agctcccgga acacttcctt gcagaagccg ttgacgatca tcgagacggc gtcctcggta
     7261 ctgaggccgc gcgccttgca gtagaaaatc tggtcttcac cgatcttgga ggtggacgcc
     7321 tcgtgctcca tcttcgccgt cgggttcttc acctcgatgt acggaaaggt gtgggcgccg
     7381 catttgtcgc caaggaggag gctgtcgcac tggctgtaat tgcgggcgcc ctccgcgccg
     7441 gccaggatct tcacctgccc gcggtaggtg ttctgcccga agccagccga gatccccttc
     7501 gacacgatgg tggagcgggt gttcttgcca atgtggatca tcttggtgcc ggtgtcggcc
     7561 tgctggtagt ggttggtgag ggccaccgag tagaactcgc cgaccgagtt gtctccctgg
     7621 aggatgcacg aggggtactt ccaggtgatt gccgagcccg tctccacctg cgtccaggag
     7681 atcttcgagt tcttcccctg gcacttgccg cgcttggtca cgaagttgta gatgccgccc
     7741 ttcccttcgt gatcgcccgg gtaccagttc tggatggtgg agtacttgat cgtggcgttg
     7801 tcgtgggcga tgagctccac gatggcggcg tgaagctggt tttcatcgcg catcggggcg
     7861 gtacacccct cgaggtagct gaccgtggcc ccttcgtcgg cgatgatcag cgtccgttcg
     7921 aactggccgg tcttgctggc attgatgcgg aagtaggtgg agagctccat cgggcagcgg
     7981 acgcccttcg ggatgtagac gaacgagccg tccgagaaga ccgccgagtt gagcgcgccg
     8041 aagtagttgt cggtgtacgg caccaccgag cccaggtact tcttcaccag ctcggggtgc
     8101 tccttcaccg cctcggagaa ggaacagaag atgattccca cctcggagag cttgtccttg
     8161 aacgtggtgg ccaccgacac cgagtcgaac acggcatcga ccgccacgcc ggccagcagc
     8221 gcgcgctcgg ccagcgggat gcccagcttc tcgtaggttt cgaggagctt gggatcgacc
     8281 tcgtcgaggc tcttcgggcc gtcggtcttc tgcctgggcg ccgagtagta gctgatggcg
     8341 ttgaagtcga tgggcgggta gtggacgttg gcccactccg ggtcggacat cgtggtccag
     8401 tgcttgtagg ccttcacccg ccactcggtg agccactccg gctccccctt cttggcggag
     8461 agggcgcgca cgacgtcctc gttcaggccc ggcggaaact ggtcggcctc gatgtccgtc
     8521 gtgaagccgt acttgtactc gcgattcgcg agttcctcga tcgtttgatt ggcggttgtc
     8581 atgcgatctc cccggccagg ggtgccgcgg ccggcgtcag gttcggaaac gacatcggcc
     8641 gggccatgtc ggccaggctg atcccgcgga gcgattgaag aacggcggcg ttgatccgat
     8701 tccagttggg ccgcgagggg caccgcccct cgtgggcgca ctccccggaa tgaacgctgc
     8761 acgtggtgag cccgatcggc ccgtcgatgg cctcgataat cgtggccacg gaaatccctt
     8821 ccggcttgcc cgccagctgg tatcccccct tgaccccgcg gtgggagatc agtagccccc
     8881 ggtggaccaa tgccttcagg atcttgccca ccatcgggag
//
LOCUS       DASVOD010000722         5059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_465521, whole genome shotgun sequence.
ACCESSION   DASVOD010000722 DASVOD010000000
VERSION     DASVOD010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465521"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2926)
                     /locus_tag="VF720_15590"
     CDS             complement(<1..2926)
                     /locus_tag="VF720_15590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880835.1"
                     /translation="MLHRYLTVLALLLSLTALPAIAQETEPGSYEPLRWRLETPWDDL
                     WSTRWNEGIFITERLSLRALRGGYQDQVSVAPGMDLLVATRNIQNIPILPPRYYTPAE
                     YAHDALAMEFGRNWRIETLKTVKGDPTLEAQRQSQGGFNLELPVELPGIAESIFGEGA
                     PNLRISGSERITIGGTSSWRTPELAVLGRKRSLFPTLEMRQELNVRLNGTIGDKLHID
                     INQNSEASTSLENQIKIYYQGYDDDVVQRVDLGNTNLTLPHSTYVSASTRQEGLFGVK
                     MTGRLASWDFAMLASKQEGQGDVASFSGGSQETKIGGTDAKVNDVDFERRKYYFLTDP
                     DSAEGRVVAGPDLEVFVSTRNAQEDNRNGRLTYRAKLSLKGRDDDALKVVDDVVKLRP
                     NDDYFFVDDTRTRFPVIALRSSLQSEYRLGVRYRYATGTSSEGIVGTFPAAGDTINLQ
                     MIKPAWDDIKGDDLLTSPWKDTRRLEAKNVYNIGARDIVDLSVLNLSIRNSSIPSQPS
                     AIDSITYLEICGLDLINNSTGRGLDSDAPSAKGPDGLVDPAYIDQTNGYIFFPDLRPF
                     DPSDVDLNDGDYRLRSRQEPRRRALGRRVEVVDGMTEYVREPSRVSETNPLIYDKRDV
                     KEEYRKYYIDGTYKAFATDINLNRPGILENSETVRMDSEVLVRGVDYSITYETGQVKL
                     ISEKARRAQGQLQITYAYTPLFTQGNRSLMGFSTTYGGSPDFDLSTTFLYETKGSTEQ
                     RPRLQQEPSRTMLADVTGSWRTRPWVLTRIADALPLVQTNAPSNLTFTGAVGMSRPNP
                     NTKNTVYVDDMEGSIQTTSAGVGQEAWQWSSRPFEDNLRDEDRLGLLWFPVQRQVLRG
                     DLSPELATAEKNDYISVLGIILAPNPSVPEGSRTWAGLTHVMSSSGLDLSEAQYLEIW
                     INDFNRFHGPNAHDGLARYLKIDLGSVSEDAVWDPKTPPAPANGRLDTEDGNYDGQRT
                     LAE"
     gene            3204..4556
                     /locus_tag="VF720_15595"
     CDS             3204..4556
                     /locus_tag="VF720_15595"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7880836.1"
                     /translation="MRILERYVLREHILPFLLGFSVVTLIIVLDFIFDYLNLIVSKGV
                     PPLVVLRLFLLALGWITVLSFPCGVLVACLMTFGRMSQDNEITALRASGVPVTRIFLP
                     VLLASVGLGILLGLFNNYVLPETNHAFATLNLAISRKSPTAFIKAGVFIDDYPDMSIL
                     VGSVDDKKGIIKDVTIYDFSQGDIPVTILARHGRTHYDAQGTALTIDLFDGEIHEVPA
                     DGDSRRYRRLFFDHHVIVMRNPGASLEIPDRDSRSEREMNVAALNAEITRLGQTAQDR
                     SRMLEADLDSLGFPDFATFERRVLPPKPVKGISSLVQALGGPKAGLKPGETHLEIPEP
                     DRDRLMTRQRDLSLIRKKINSHVVEVQKKYSIPFACVVFVLLGAPLGMRTRKGGMATS
                     SISIVVFIVYYLLLIGGEQLADRSVVPPALAMWTPNIVFGIPGLILTWRALTGADDRR
                     "
     gene            4553..>5059
                     /locus_tag="VF720_15600"
     CDS             4553..>5059
                     /locus_tag="VF720_15600"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEX7880837.1"
                     /translation="MIGRMLDRYILKQMIPFYFLGVAAFALFFITADAFERIDVFVDN
                     HTPVALIIRYYAAGSVYNAILVSPIALLLAIFFVFGQMTRFNEVTAMKTAGLSLYRIF
                     VPVWLLALLISGGCWWVADSLMPRANQQKKEIYNEKIRGRPPRTGGVRMNLNYLGPAG
                     RIWAVRRYH"
BASE COUNT          826 a         1632 c         1606 g          995 t
ORIGIN      
        1 cctccgccag cgtccgctgc ccgtcgtagt tcccgtcttc cgtgtccagt cggccgttcg
       61 ccggcgcggg aggcgtcttc ggatcccaca ccgcgtcctc gctgacgctc ccgagatcga
      121 ttttcaggta cctggcaagc ccgtcgtgcg cattgggtcc gtggaaccgg ttgaagtcgt
      181 tgatccatat ctcgagatac tgcgcctccg acaggtcgag gccggacgac gacatcacat
      241 gcgtgaggcc ggcccacgtc cggctaccct ccggcacgga tggattgggg gccaggatga
      301 ttccgagcac gctgatgtag tcgttcttct ccgcggtggc cagttcgggc gaaaggtcgc
      361 cgcgcagcac ctggcgctgc accgggaacc agagaagccc caggcgatcc tcgtcgcgca
      421 ggttgtcctc gaacggacga ctcgaccact gccacgcctc ctggccgacg cccgcggagg
      481 tcgtctggat ggagccttcc atgtcgtcca cgtacacggt gttcttggtg ttcgggttgg
      541 gccgcgacat gcccacggct cccgtgaacg tgaggttcga gggtgcgttc gtctggacca
      601 aaggcagcgc atccgcgatg cgggtgagca cccaggggcg cgtccgccag gagccggtca
      661 cgtcggccag catggtgcgc gacggctcct gctgcaatcg tggccgctgc tcggtggaac
      721 ccttggtttc gtagaggaag gtggtcgaaa gatcgaagtc gggcgaaccg ccgtacgtcg
      781 tcgagaagcc catcaggctc cggttcccct gggtgaagag cggcgtgtag gcgtaggtga
      841 tctgcaactg tccctgcgcc cgccgggcct tctccgaaat cagcttcacc tgtccggttt
      901 cgtaggtgat ggagtagtcg acgccacgga ccagaacttc gctgtccatg cggaccgttt
      961 cggaattctc gaggatcccg gggcggttga ggttgatgtc ggtggcgaag gccttgtaag
     1021 taccgtcaat gtagtacttg cggtactcct ccttcacgtc gcgcttgtcg tagatcaagg
     1081 ggttcgtctc ggacacgcgc gacggctccc gcacgtattc cgtcattccg tccaccacct
     1141 cgacccggcg tccgagcgcg cggcgacgcg gttcctgccg actgcgcagc cggtaatcgc
     1201 catcgttcag gtccacgtcg ctgggatcga aggggcggag atcgggaaag aagatgtatc
     1261 cgttcgtctg gtcgatgtac gcggggtcga ccaggccgtc cgggcccttg gcgctcgggg
     1321 cgtccgagtc cagcccacga ccggtcgaat tgttgatcag gtcgaggccg cagatttcca
     1381 ggtaggtgat gctgtcgatg gccgacggct ggctggggat ggaactgttg cgaatgctga
     1441 ggttcagtac cgacaggtcg acgatgtccc gcgccccgat gttgtagaca ttcttggcct
     1501 cgaggcgccg ggtgtccttc caggggctcg tcaggagatc gtctcccttg atgtcgtccc
     1561 aggccggctt gatcatctgc agattgatgg tgtcgcccgc ggccggaaac gtcccgacga
     1621 tcccttcaga actcgtgccg gtcgcgtatc ggtagcgcac ccccaaccgg tactccgact
     1681 gcagcgagct gcgcagcgcg atcaccggga accgcgtccg ggtgtcgtcc acgaaaaagt
     1741 agtcgtcgtt gggcctcagc ttgaccacgt cgtcgaccac cttgagtgcg tcgtcgtccc
     1801 gccccttgag cgacaacttg gccctgtagg tcaggcgccc gttgcgattg tcttcctggg
     1861 cgttgcgcgt gctcacgaac acttcgaggt cggggccggc caccacgcgc ccctcggcgg
     1921 aatccgggtc ggtcaggaag tagtacttgc ggcgctcgaa gtcgacgtcg ttgaccttgg
     1981 catcggtgcc gccgatcttc gtctcctggc tgccgccgga gaagctggcc acgtccccct
     2041 gcccctcctg cttgctggcc agcatggcaa agtcccagct ggccaggcgg ccggtcatct
     2101 tgacgccgaa cagcccttcc tgccgggtcg aagccgacac gtacgtggag tgcgggagcg
     2161 tgaggttggt gtttccgagg tccactcgct gcaccacgtc gtcgtcgtaa ccctggtaat
     2221 agatcttgat ctggttctcg agcgaggtgg aggcttccga gttctggttg atgtcgatgt
     2281 ggagcttgtc gccgatcgtg ccgttgaggc gcacgttcag ctcctggcgc atctcgagcg
     2341 tgggaaagag cgagcgtttg cggccgagga cggcgagctc gggtgtgcgc cagctggagg
     2401 tgcccccgat cgtgatgcgc tcgcttccgg aaatgcggag gttgggggcg ccttcgccga
     2461 agatgctttc ggcgatgccg gggagctcga ccggcagctc gagattgaac ccaccctgcg
     2521 actggcgctg ggcctcgagc gtcggatcgc cctttaccgt cttgagcgtc tcgatgcgcc
     2581 agttccgtcc gaactccatg gcgagcgcgt catgggcata ttcggccggt gtgtaatagc
     2641 gcggggggag gatcgggatg ttctggatat tgcgtgtggc aaccaggaga tccatgccgg
     2701 gtgccaccga aacctggtcc tggtagccac cgcgaagggc tcggagcgag aggcgctccg
     2761 tgatgaagat tccctcgttc cagcgcgtgc tccagaggtc gtcccagggg gtttcgaggc
     2821 gccagcggag gggttcgtag ctgccgggtt cggtttcctg ggcgatggcc ggcagtgccg
     2881 tgagactgag gaggagcgcg aggaccgtca ggtagcgatg gagcaatccg tacctctcgt
     2941 ctccccgtga acggccgtca tgtcccggct ggggcggatg ctgacccgcc gtggtccgta
     3001 actattgatg tatcagaaag ttagcaaagc atcggtagag tgtcaaacgg ccggcttcat
     3061 tgtgccggcg ggccgccgca cctatactgc cgcgccgaac ggaggcactc acgagggcgg
     3121 gacgaccggg gagatgcagg cgaatcgagc cgccggaaaa ccagcggtgg aaccgggcta
     3181 acctacccaa gtttcaactt tttatgcgaa ttctcgagcg ctatgttctg cgcgaacaca
     3241 tcctcccgtt cctcctcgga ttcagcgtcg tcacgctgat catcgtcctc gatttcattt
     3301 ttgattatct caacctgatc gtcagcaagg gtgtgcctcc cctggtggtc ctccggctct
     3361 tcctcctggc cctggggtgg atcaccgtcc tatcattccc gtgcggcgtg ctggtggcct
     3421 gcctcatgac gttcggccgg atgtcgcagg acaatgaaat caccgcgctc agggcctccg
     3481 gggtcccggt cacccggatc ttcctgccgg tgctcctggc ctccgtcggc ctcgggatcc
     3541 tcctcggact cttcaacaac tacgtccttc ccgaaacgaa ccacgccttc gccacgctca
     3601 acctggccat cagccggaag tcccccacgg ccttcatcaa ggccggcgtc ttcatcgacg
     3661 actatcccga catgagcatc ctggtcgggt cggtcgacga caagaaggga atcatcaagg
     3721 acgtcaccat ctacgacttc agccaggggg acatcccggt gacgatcctg gccaggcacg
     3781 ggcgcaccca ctacgatgcg caggggacgg ccctcacgat cgacctgttc gacggcgaga
     3841 tccacgaagt ccccgcggac ggcgactcgc gtcgataccg tcgcctcttc ttcgaccacc
     3901 acgtcatcgt gatgcggaat ccgggagcgt ccctcgagat tcccgaccgg gactcccgct
     3961 ccgagcgcga gatgaacgtg gcggccttga atgcggagat cacgcggctc ggccagacgg
     4021 cccaagaccg cagccgcatg ctcgaagccg atctcgacag cctcggtttc cccgacttcg
     4081 ccactttcga gcgccgcgtg cttcccccga agccggtcaa gggaatctcg tccctcgtgc
     4141 aggcgctggg tggccccaag gcggggctca agccaggcga aacccatctc gagattcccg
     4201 agccggaccg cgaccggctg atgacccgcc aacgcgacct gagcctcatc cggaagaaga
     4261 tcaacagtca tgtggtcgag gtccagaaga aatactcgat accgttcgcc tgcgtcgtct
     4321 tcgtcctcct cggtgcgccg ctgggcatgc ggacacgaaa gggtgggatg gccaccagct
     4381 ccatcagcat cgtggtcttc atcgtctatt acctgctcct gatcggtggg gagcagctcg
     4441 ccgaccggtc ggtggtgccg ccggccctgg ccatgtggac accgaacatc gtcttcggca
     4501 tcccgggcct catcctgacg tggcgggccc tgacgggagc ggacgaccgc cgatgatcgg
     4561 acggatgctc gaccgctaca tcctgaagca gatgattccg ttctacttcc tcggcgtggc
     4621 ggccttcgcc ctcttcttca tcacggccga cgccttcgag cggatcgacg tcttcgtcga
     4681 caatcacacg cccgtggcgc tgatcatccg gtattacgcc gccggaagcg tgtacaacgc
     4741 cattctcgtc tccccgatcg cgctcctgct cgccattttc ttcgtcttcg ggcagatgac
     4801 gcgcttcaac gaggtcacgg ccatgaagac ggccggcctc tccctctacc ggatcttcgt
     4861 gccggtgtgg ctccttgcgc tcctcatcag cggcggctgc tggtgggtgg ccgactccct
     4921 gatgccacgc gccaaccagc agaagaagga gatctacaac gagaagatcc gcgggcgccc
     4981 gccgcgcacc ggcggcgtgc gcatgaatct caactacctc ggtcccgccg gaaggatatg
     5041 ggcggtgcgc cgctaccac
//
LOCUS       DASVOD010000723         4383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_671978, whole genome shotgun sequence.
ACCESSION   DASVOD010000723 DASVOD010000000
VERSION     DASVOD010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671978"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VF720_15605"
     CDS             complement(<1..659)
                     /locus_tag="VF720_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX7880838.1"
                     /translation="MIVRQSPRRACPWCFTGTPPSADSTGGYWHFPCSGTSRSWLDLF
                     LSIAANAGGRVMGWLPSIACCRAWPGRWFAPVVGLIVVGLSDIGGALPARAAWQNFKG
                     SRDGLADSQVLAIHEAPDGSLWFGTPRGPSHYDGLRWTSPDLGTTNPVVQCITPTSNG
                     NLWFGLQGGGAVLFHTGNGRHEILSSGDGPLPSDNVQAILEDRAGDTWFGTPNGLARR
                     VA"
     gene            complement(803..>4383)
                     /locus_tag="VF720_15610"
     CDS             complement(803..>4383)
                     /locus_tag="VF720_15610"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880839.1"
                     /translation="GIAPASAPVRTESSHLCTASIGVPLQVTLAVDPASIVVGGTVMV
                     QGHLESNAQLASIDVEKSATGAARVLDGGKRPLDGSLNSEGQLDFTVPVVLEGTEPSE
                     VTVAIEARDAASGQVYTKNETIAFYNHNGRWYADMGIVLQLRIRAIEDDYNAGRLSED
                     EARAATRELARVDAEVSDAPVVRPLRTAEEQSLVDRLALAPADVPVSKSPGSVQGTCI
                     TIEGHIGWRDENNNLHDAFGLSVQIWDEDTVIDEFVDVTATDTNGNYHIEVNGDDGFL
                     QGDRDIYVRIISANTAVSIETAGLFGSVYENVSGVSDETPCGTTITRSVNFVNTDPTP
                     AMQLCESGTWVASYVADQLNGDSFLGQIVLEWPGTTTSANYDGSDINLRPGDRWDWDV
                     LHHEYGHYVQDVFNTEDNPGGPHNIGDCVSDVHNSKSEGIRLAWGEGWPTYNGTNAQL
                     FYNLASRSIPRVGDANYADSGESNFNYNLDDQDSNGMGEDNEVAVQRILWDWNDGASD
                     SRDAVNLSEVDLFNWINAADPTSFSAAWAAIRPNLSAANLLAAAEVLSDHFVGPRLTA
                     PAENAIVSPSNNNNLSWTRGAGCSSVYDGDGFTVRFFRADNSAPILSIPVGNTTSHAL
                     TLGELQTLVAATHNVLWGVEASNSSSPGTGPYLGETFAITVNRPPDADAGDDVTAECS
                     SQTTTPVQLDGTGSSDPDGDALTYTWSAPGVTWDNNHSATPTGNFPFGTKVVTLEVSD
                     GIETDTDQVNVTIEDTTPPEITCPVAVTIECNDHCEGGGVPKDDAQLTDFFTGVSATD
                     ICDTDITFANDAPDCFPVGTTDVVFTATDGSNNAASCTTSVTVEDTTPPEITVVLSRD
                     ALWPPNHKMADISAEVTVTDICDPNPTFVLTSVTSDEPDNGLGDGDTPNDIQGVTYGE
                     ADTEFQLRSERAGNRDGRKYTIIYTASDISGNTTLDTTCVFVPHDQRGHANTMAGFLP
                     VGQALDPSVIEFRLVIPSRANFNALTVPGATAIVGNSIDAMRPLRFEYRDVNGDHEVD
                     LSLWYDTKSVIALRDNSNRLDPVGLHYWGSDGQGYLLNSIVKLEPMIEVVAAAGSENS
                     DRPEDGSEVEGEAPPIGTDGGVLVTGFDPHGALVLAEPGPVTVDLFDVTGRKVKTLIT
                     GDLSAGTHELSWDGRDDAGRAVASGLYFYRVITPEGVFSRKLFVSR"
BASE COUNT          676 a         1370 c         1561 g          776 t
ORIGIN      
        1 gccacccgcc gggccagtcc gttcggcgtg ccgaaccagg tatcgcccgc gcgatcctcg
       61 aggatggcct gcacgttgtc gcttggaagc gggccgtccc cgctcgaaag aatctcgtgg
      121 cggccgttgc cggtgtgaaa cagcacggcg ccgcccccct gcaggccgaa ccaaaggttc
      181 ccgttgctgg tgggcgtgat gcactgcacg accgggttcg tggtgccgag gtcgggcgag
      241 gtccagcgaa ggccgtcgta gtgggaaggt ccgcgcggcg tgccgaacca gaggctgccg
      301 tccggggctt cgtggatggc gagcacctgg ctgtcggcca ggccgtcgcg cgaacccttg
      361 aagttctgcc aggccgctcg ggcgggaagc gcaccgccga tatcgctcag gccgacaacg
      421 atcaggccga cgacgggcgc gaaccaacgc ccgggccagg cccggcaaca cgcaatcgat
      481 ggcagccacc ccatgacccg ccctcccgca ttcgcggcga tggagaggaa gagatccaac
      541 caggaacgtg acgttcccga gcaagggaag tgccagtagc cgcccgtgga gtccgcgctc
      601 ggcggcgtgc ccgtaaagca ccacggacag gcgcgacgcg gcgattgccg cacgatcatg
      661 gccacatcgc acaggctgcc gatccacgac gcgactgcgg aaatcgccgg aaaacggcga
      721 atgaatgcga acggggcgcc cccggatggg agcgccccgt tcaagtcgga acgaacgccg
      781 ggccgttgag cggcccgtat ggttaacgac tcacgaacag cttccgcgag aagacgcctt
      841 ccggcgtgat cacacggtag aaatagagcc cgctggccac cgcgcgcccc gcgtcgtcgc
      901 ggccgtccca cgaaagctcg tgggtgccgg ccgagaggtc gccggtgatc agggtcttga
      961 ccttgcggcc cgtcacgtcg aagagatcga cggtgaccgg acccggctcg gcaagaacca
     1021 gcgccccgtg gggatcgaag ccggtgacga gcacgccgcc gtccgttccg atcggcggtg
     1081 cctcaccttc cacttcgctg ccgtcctcgg gacgatcgct gttttcactg ccggcggccg
     1141 ccacgacttc gatcatcggt tcgagcttca cgatgctgtt cagcaggtag ccctggccgt
     1201 cggagcccca gtaatggagg ccgaccggat cgaggcgatt cgaattgtcg cgcagcgcga
     1261 tgaccgactt cgtgtcgtac cagagggaga ggtcgacttc gtgatcgccg ttcacgtcgc
     1321 gatactcgaa ccgaagcggg cgcatggcat cgatcgaatt gccgacgatg gccgtggcgc
     1381 ccggaacggt aagcgcattg aaattggcgc gtgacggaat gacgaggcgg aactcgatga
     1441 ccgacgggtc gagcgcctgc cccaccggca ggaagccggc catcgtgttg gcgtggccgc
     1501 gctggtcgtg cggcacgaac acgcaggtgg tgtcgagcgt cgtgttgccc gagatgtcgc
     1561 tggcggtgta gatgatcgtg tacttccggc cgtcccggtt gccggcccgc tcggaacgga
     1621 gctggaactc ggtgtcggcc tcgccgtacg tcactccctg gatgtcgttg ggggtgtcgc
     1681 cgtcgcccag gccgttgtcc ggctcgtccg acgtgaccga cgtgaggacg aaggtcgggt
     1741 tcgggtcgca gatgtcggtc accgtcacct cggccgagat gtcggccatc ttgtgattgg
     1801 gcggccagag ggcatcgcgg ctgagcacca cggtgatctc ggggggcgtc gtgtcttcca
     1861 ccgtcacgct ggtggtgcag ctggcggcgt tgttgctccc gtcggtggcg gtgaagacca
     1921 cgtccgtggt gccgaccggg aagcagtcgg gcgcatcatt ggcaaacgtg atgtcggtgt
     1981 cgcagatgtc ggtggccgac acaccggtga agaagtcggt gagctgcgcg tcgtccttgg
     2041 gcacgccgcc accctcgcag tggtcgttgc actcaatggt gacagccacc gggcaggtga
     2101 tttcgggggg cgtggtgtcc tcgatggtca cgttcacctg gtcggtgtcg gtttcgatgc
     2161 cgtccgagac ttccagcgtg accaccttgg tgccgaaggg gaagttgccc gtcggcgtgg
     2221 ccgagtggtt gttgtcccag gtgacgccgg gggcgctcca ggtgtaggtc agggcatcgc
     2281 cgtcggggtc gctcgagccg gtgccgtcca gctgcaccgg cgtggtcgtc tgcgacgagc
     2341 actcggccgt cacgtcgtca ccggcgtcgg catcgggcgg ccgattgacg gtgatggcga
     2401 acgtctcgcc caggtacggc ccggtgcccg ggctggacga gttcgaggcc tccacgcccc
     2461 acagcacgtt gtgcgtggcg gccaccagcg tctggagctc gccgagcgtc agcgcgtgcg
     2521 aggtggtgtt gccgaccggg atcgacagga tcggggcgga attgtcggcc cggaagaagc
     2581 ggacggtgaa gccgtcgccg tcgtacaccg agctgcaccc cgcgccgcgg gtccacgaca
     2641 ggttgttgtt gttcgacggg gagacgatgg cgttctcggc cggcgcggta agcctcggcc
     2701 cgacgaagtg gtcggacagg acttccgccg ccgccaggag attggccgcc gagaggttcg
     2761 gccggatggc cgcccaggcc gccgagaacg aggtcgggtc ggcggcgttg atccagttga
     2821 acaggtcgac ttccgacagg ttgaccgcgt cgcggctgtc ggacgcgccg tcgttccagt
     2881 cccacaggat tcgctgcacg gcgacttcgt tgtcctcgcc catgccgttc gagtcctggt
     2941 cgtccaggtt gtagttgaag ttgctttcgc cggaatcggc gtagttcgca tcaccgacgc
     3001 gggggatgct ccgggaagcc aggttgtaga agagctgggc gttggtgccg ttgtaggtgg
     3061 gccatccttc gccccaggcc agccggattc cctcggactt ggagttgtgc acgtccgaga
     3121 cgcagtcgcc gatgttgtgc ggtccgcccg ggttgtcctc ggtattgaag acgtcctgca
     3181 cgtagtgacc atactcgtgg tgcagcacgt cccagtccca gcggtcgccg ggacgcaggt
     3241 tgatgtccga gccgtcgtag ttggccgagg tggtggttcc cggccattcc agcacgatct
     3301 ggccgaggaa gctgtcgccg ttgagctggt cggccacgta ggacgcgacc caggtgcccg
     3361 attcgcacag ctgcatggcc ggcgtgggat cggtgttcac gaagttcacg ctgcgggtga
     3421 tcgtggtgcc gcacggcgtt tcatcgctga cgcccgacac gttttcgtac acggagccga
     3481 agaggccggc ggtctcgatg gaaaccgcgg tgttggccga gatgatgcgc acgtagatgt
     3541 cgcggtcccc ctgcaggaaa ccgtcgtcgc cgttcacctc gatgtgataa ttgccgttgg
     3601 tgtcggtggc ggtcacgtcc acgaactcgt cgatgacggt gtcctcgtcc cagatctgca
     3661 cgctgagtcc gaaggcgtcg tggagattgt tgttctcgtc ccgccagccg atgtgtccct
     3721 cgatcgtgat gcaggtcccc tggacgctgc cggggctctt ggacaccggc acatcggcgg
     3781 gcgccagcgc cagccggtcg accaggctct gctcctcggc cgtacggagc ggccggacga
     3841 cgggggcatc gctcacttcc gcatccaccc tggcgagttc gcgggtggcg gcgcgggctt
     3901 cgtcttccga caaccggccg gcgttgtagt cgtcctcgat ggcgcggatc ctgagctgga
     3961 ggacgatgcc catgtcggcg taccagcgac cattgtggtt gtagaaggcg atggtctcgt
     4021 tcttcgtata tacctggccg ctggcggcgt cacgcgcctc gatggccacg gtcacttcgg
     4081 aaggttctgt cccctcgaga acgacgggga cggtgaagtc gagctgtcct tcggaattga
     4141 gcgagccatc gagtggccgc ttgccgccgt ccagtacgcg ggccgcgccg gtggcggatt
     4201 tctccacgtc gatggaggcg agctgggcgt tcgattcgag gtggccctgc accatcacgg
     4261 ttccgccgac cacgatggag gcgggatcaa ccgcgagggt gacctgcagg ggcacgccga
     4321 tggatgccgt gcagaggtgg gacgattccg ttcgcaccgg ggccgaggcc ggcgcgatcc
     4381 cgg
//
LOCUS       DASVOD010000724         7005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_154258, whole genome shotgun sequence.
ACCESSION   DASVOD010000724 DASVOD010000000
VERSION     DASVOD010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154258"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VF720_15615"
     CDS             complement(<1..1393)
                     /locus_tag="VF720_15615"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEX7880840.1"
                     /translation="MRPVTFLFGIHNHQPVGNFDWVIEEAYQKAYRPFLDRLEAHPGV
                     KVALHNSGILLDWFGKHHPEYLERVGSLVAAAQVEPITGGYYEPILPVIPDADKTGQI
                     RRMSEWIDAHWGKRPTGLWLAERVWEPSLAVPIAEAGVRWLMVDDAHFLAAGAEPERL
                     WNWWLTEDQGTTLGVWPISKALRYLIPFEPPEKTIEFLRNLAAGGADRVAFLADDGEK
                     FGVWPDTFERVYTNGWLEKFFCLLEANADWLKMELPGAVTATRAPAGRIYLPTASYSE
                     MMEWALPTPVQKEFHRVRERLEQARAPEAGFVRGGFWRNFLARYPESNLMHKRMLDAR
                     QRLDANGFSNETRALVLDDVWQAQCNCAYWHGVFGGLYLPHLREGVYRRILSAEARLD
                     ESGSAGLPFLRVEHSDLDRDGHDEIRMVNRGVGVIVSPFRGGAIESMDYYPRLRNLSH
                     GLTRRPEAYHEALR"
     gene            complement(1390..1878)
                     /locus_tag="VF720_15620"
     CDS             complement(1390..1878)
                     /locus_tag="VF720_15620"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEX7880841.1"
                     /translation="MSLLLVRIDDRLIHGQVSVGWAGQLKPDLILVLDDEVAADDWEN
                     DLVCSACPDSVTARVMPVEAGVAWLAGEEARSKKIVVLVRGTKQARALVEAGLPLATV
                     NVGGLHSHAGSRSFLPYVYLDDAEIADLRWLAGRGVMLNAQDLPGNKGIDLAPLIAGN
                     GA"
     gene            complement(1875..2321)
                     /locus_tag="VF720_15625"
     CDS             complement(1875..2321)
                     /locus_tag="VF720_15625"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880842.1"
                     /translation="MTAVPADPSAGDASPPKPRVPALLVGHATFAASLLEAASAIAGP
                     VDDIPCLTNSGLAADALADSIGSAVTSLGLPAVLLIDVPGGSCFNAARRATRGQAGAH
                     LVCGVNLPLLLDFLMMRDQLPAAELVEHIIERGRRGISACRGDLGS"
     gene            complement(2318..3310)
                     /gene="hprK"
                     /locus_tag="VF720_15630"
     CDS             complement(2318..3310)
                     /gene="hprK"
                     /locus_tag="VF720_15630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX7880843.1"
                     /translation="MVPTAGRVDSVKEIPVAQLIEQKELELTLLTESIESTVPITVSD
                     INRPGLAMAGFVENFLVERIQVIGQTEVALLDSLDEDGRELAVERLLQFQVPCIVVAK
                     DLELPAVLLERAARRKIPVLRTPMSTTPFIHELTAYLDDVFAPTIAVHGSLVDVYGVG
                     LLFTGRSGIGKSECALDLVERGHRLVADDVVTVKRRHTSVLIGQANQTLGHYMEIRGI
                     GLLDLQSIFGIRAIRFQKRIEVEVRLEDWDSERAYERVGLDDALTTHLGVKIPIVTVP
                     INPGKNITVIGEVIALNYLVKAYGFDPAREFERRHREMMTTNARLKRLARDDEE"
     gene            complement(3313..3678)
                     /gene="raiA"
                     /locus_tag="VF720_15635"
     CDS             complement(3313..3678)
                     /gene="raiA"
                     /locus_tag="VF720_15635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX7880844.1"
                     /translation="MRLQITTRHYDLPTDLKGLTEERLTKLKRFFDAIMDVNLVLSTE
                     KHRNHAEVVIRAGGHDWVGTAEAGDMRTAIDDAVDRIEIQLKKHKDRLTDRKGRTSLG
                     EAMTDGANTDSTGAVEIEE"
     gene            complement(3701..5143)
                     /gene="rpoN"
                     /locus_tag="VF720_15640"
     CDS             complement(3701..5143)
                     /gene="rpoN"
                     /locus_tag="VF720_15640"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX7880845.1"
                     /translation="MEMRQGLRLTQTQKLVMTPKLQQALKLLQAPTLELQQMLKAEMV
                     SNPLLEEIDEITEEPEPEKATAEDDFGEEGRLEREEQEPLEAPEAPEEVDWTEFMQQG
                     SDASTTRSEERHEEFVERVPISRPSLDEHLAEQLRYKGMGDEAEAIGDYLIGSIDERG
                     YLTMTVEEVAAELDKPVETIESVLKAIQALDPPGIGARDLRECLLLQLRARGQEGELS
                     WRIVDKHFDDLVRRRQIDIARSLKVPVEEVQTALDLIGTLSPVPGNQVSGADAQYVYP
                     DLIVERVGEDYVFTLNDRKVPRLRICSAYEHVLKDGAPKNGETRDYVVSKLNSARWLI
                     QTIEQRRKTMVKVMRAIVEEQREFFDSGILHLKPLTLQDIASKIGMHESTVSRVTSGK
                     YVQTPRGVFELKYFFSSGLNTQGGDDISAKSAKAIIAKLIEGEDKHDPLSDQKLMEML
                     QSQGLDIARRTVAKYREQLNVPNARFRKRH"
     gene            complement(5182..5955)
                     /gene="lptB"
                     /locus_tag="VF720_15645"
     CDS             complement(5182..5955)
                     /gene="lptB"
                     /locus_tag="VF720_15645"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEX7880846.1"
                     /translation="MTRPAEMTRPTEPAILPGPHLMAEGLVRYYGKFKVVDGVSLSVE
                     PGQVVGLLGPNGAGKTTTFYMIVGLLKPKAGTIRIGDTDITQMPMYRRARLGVGYLPQ
                     EPSVFRKLSVKDNIQAILETIPMSKGDRSARLDELLAELDMTRLADRKAWNLSGGERR
                     RLEITRALATRPSFLLLDEPFVGIDPIAVAEIQEIVLRLKAKGLGILITDHNVRETLS
                     STDHAFIMFQGRIMLSGTADQLAADPEARRLYLGEKFQL"
     gene            complement(5939..>7005)
                     /gene="lptC"
                     /locus_tag="VF720_15650"
     CDS             complement(5939..>7005)
                     /gene="lptC"
                     /locus_tag="VF720_15650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEX7880847.1"
                     /translation="VHGLSKPVREARLDAWLDAHRASLGSRTIKVGTMSGAREAHRVL
                     TAGGVLGLLIDHRVRSGGRWIDFFGRPSRFATGPARLAIATGASMLPIVIRRRSDGGH
                     RVVVGAALASAATGSPASRADSLLEAAVRALEAMIRIDPASWAWMHPRWGRPSQVPAG
                     AAASVARELSSAARMVAMWTALAILSTGVQSCGSKPDGNTAPPAVGEPSSMLSGVTLR
                     ETLDGRLRWALKADSSETFTQPNRTVASNIHVDFYDEAGQPSSFLTADEGVVQRSNND
                     MTARGHVVVITTKGDTLTTERLDWSSVQNRVKTDLPFRLGRPDGVFTGVGFESDPDLT
                     HYTTQQVKIDARDATDDAPR"
BASE COUNT         1101 a         2348 c         2322 g         1234 t
ORIGIN      
        1 cccgcagcgc ctcgtgatac gcctcggggc gtcgcgtgag cccgtgtgac aggttgcgaa
       61 gccgcgggta gtagtccatc gactcgatcg cgccaccccg gaacggcgac acgatcactc
      121 ccaccccgcg gttcaccatc cggatctcgt cgtgcccgtc ccggtcgaga tcggaatgtt
      181 cgacgcggag gaaggggagc cccgcggagc ccgactcgtc gagccgggct tcggccgaca
      241 ggattcgccg gtacacccct tcacgcaggt gcggcaggta gaggccgccg aagacgccgt
      301 gccagtaggc gcagttgcac tgcgcctgcc acacgtcgtc gaggaccagc gcgcgcgttt
      361 cgttgctgaa gccgttcgcg tcgagccgct gtcgcgcatc gagcatccgc ttgtgcatca
      421 ggttcgactc gggatacctg gccaggaaat tccgccagaa gccgccgcgc acgaaaccgg
      481 cctcgggcgc cctggcctgt tcgaggcgct cgcgcacccg gtggaactcc ttctgcaccg
      541 gggtggggag ggcccactcc atcatctcgc tgtaggaggc tgtcggcagg tagatgcggc
      601 cggcgggcgc tctcgtggcc gtgacggcgc cgggcaactc catcttgagc cagtcggcgt
      661 tggcctccag gaggcagaag aacttctcca gccacccgtt cgtgtacacc cgctcgaagg
      721 tgtcgggcca cacgccgaac ttttcgccgt catcggccag gaacgccacc cgatcggcgc
      781 cgccggccgc caggttgcgc aggaactcga tcgtcttctc cggcggctcg aaggggatga
      841 ggtagcgcaa cgccttggag atgggccaaa cgccgagcgt cgtcccctgg tcctcggtga
      901 gccaccagtt ccagaggcgt tccggttcgg cgccggcggc caggaagtgc gcgtcgtcca
      961 ccatgagcca gcggacgccg gcctccgcga tcggcaccgc aagcgacggc tcccataccc
     1021 gctcggcgag ccagagaccc gtcggccgct tcccccaatg cgcgtcgatc cactcgctca
     1081 tccggcgaat ctgtcccgtc ttgtccgcgt ccgggatgac cggaaggatc ggttcgtagt
     1141 agccgccggt gatcggctcg acctgtgcgg ccgcgaccag cgaacccacg cgctcgagat
     1201 actccggatg atgcttcccg aaccagtcga gcaggattcc cgaattgtgg agcgccacct
     1261 tcacgccggg atgcgcctcg agtcgatcca ggaaggggcg ataggccttc tggtaggcct
     1321 cctcgatcac ccagtcgaag ttgccgacgg gctggtgatt gtggatgccg aacaggaagg
     1381 tgaccggcct cacgcgccgt ttccggcaat gagcggggcg aggtcgatcc ccttgtttcc
     1441 ggggaggtcc tgcgcgttga gcatcacacc gcggccggcc agccacctca agtcggcgat
     1501 ctcggcatcg tccaggtaga cgtagggaag gaagctccgg ctccccgcgt gggagtgcag
     1561 cccccccacg ttcaccgtgg cgagcggaag gcccgcctcg accagcgcgc gggcctgctt
     1621 cgtgccgcgc acgagcacca cgatcttctt cgagcgcgcc tcttcaccgg ccagccatgc
     1681 cacgccggcc tcgacgggca tcacacgcgc ggtcaccgag tcggggcagg ccgaacagac
     1741 aaggtcgttc tcccagtcgt cggccgccac ctcgtcatcg agtacgagaa tgaggtccgg
     1801 cttcagctgc cccgcccacc ccaccgaaac ctggccgtgg atcaggcgat cgtcgatacg
     1861 gaccaggagg aggctcatga gccgagatct ccccggcagg ccgaaatccc ccgccggccg
     1921 cgctcgatga tgtgctccac cagttccgcc gcgggcaact ggtcgcgcat catcaggaag
     1981 tcgaggagga ggggcaggtt cacgccgcac acgaggtgcg caccggcctg gccgcgcgtg
     2041 gcgcggcggg cggcgttgaa acatgagccg ccgggaacgt cgatcaacag gacggccggg
     2101 agcccaagcg acgtcaccgc agacccgatc gagtcagcca gcgcatcggc ggcaagcccg
     2161 ctgttggtaa ggcacggaat gtcgtccacc ggaccggcaa tggccgatgc cgcctcgagg
     2221 agcgaggccg cgaaagtggc atgaccgacg agcagtgccg ggacgcgggg cttcggaggc
     2281 gacgcgtcgc cggcggacgg gtcggccggc accgccgtca ttcctcgtcg tcccgggcca
     2341 gccgcttcag gcgcgcgttg gtcgtcatca tttcccgatg ccggcgctcg aactccctgg
     2401 ccggatcgaa accgtaggcc ttcacgaggt agttgagcgc gatcacctcg ccgatgaccg
     2461 tgatgttctt cccgggattg atcgggaccg tcacgatcgg gatcttcacg ccgaggtgcg
     2521 tggtgagcgc gtcgtcgagt cccacccgct cgtaggcccg ctcgctgtcc cagtcctcaa
     2581 gccgcacttc cacctcgatg cgcttctgga accggatggc ccggatgccg aagatgctct
     2641 gtaaatcaag caggccgatg ccgcggattt ccatgtagtg accgagagtc tggttggcct
     2701 ggccgatcag gacgctggtg tgccggcgct tgacggtgac cacgtcgtcg gccaccaggc
     2761 gatgcccgcg ctccaccagg tcgagcgcgc actccgactt gccgatgccg ctccggccgg
     2821 tgaagaggag gccgacgccg tacacatcga ccagcgatcc atggaccgcg atggtcgggg
     2881 cgaagacgtc gtcgaggtag gcggtcagct cgtggatgaa cggcgtggtc gacatcggcg
     2941 tgcggagcac cgggatcttc cggcgcgccg cccgctcgag caggacggcc ggcaattcca
     3001 ggtctttggc caccacgatg cacggcacct ggaactgcag gagccgttcg acggccagct
     3061 cccgcccgtc ctcgtccagg ctgtcgagca gggccacctc ggtctggccg atcacctgga
     3121 tccgttccac caggaagttc tcgacgaagc ccgccatggc gaggcccggc cggttgatgt
     3181 cgctcaccgt gatggggacg gtcgactcga tcgactcggt caggagcgtc agctcgagct
     3241 ccttctgctc gatgagctgg gcgaccggta tttccttgac cgagtcgacg cggcctgcgg
     3301 ttggcaccac gcctactcct cgatctccac cgcgccggtg ctgtcggtgt tcgcaccgtc
     3361 ggtcatcgcc tcgccgaggc tcgtccgccc cttgcgatcg gtcaggcggt ccttgtgctt
     3421 cttcagctgg atctcgatac gatcgaccgc gtcgtcgatg gccgtccgca tgtcaccggc
     3481 ttcggcggtc ccgacccagt cgtggccgcc ggcccggatc acgacttcgg catgattgcg
     3541 atgcttctcg gtggagagga cgaggttcac atccatgatc gcgtcgaaga atctcttcag
     3601 tttcgtgagc cgttcttccg tcaggccctt caagtcggtg ggaaggtcgt agtggcgggt
     3661 cgtgatctgg agtctcatct tgccttacct cccttgggcg tcagtgacgc ttgcggaaac
     3721 gggcattggg gacgttcaac tgttcacggt acttggccac cgtgcgccgg gcgatgtcca
     3781 gcccctggga ctggagcatc tccatcagct tctggtcgga cagcggatcg tgcttgtcct
     3841 cgccctcgat cagcttggcg atgatcgcct tggcgctctt ggccgagatg tcgtcgcccc
     3901 cctgcgtgtt gagcccgctc gagaagaagt acttgagctc gaacacgccg cggggcgtct
     3961 ggacgtactt gccgctggtc acgcggctga cggtcgattc gtgcatgccg atcttgctgg
     4021 cgatatcctg gagcgtgagc ggcttgaggt gcaggatgcc gctgtcgaag aattcgcgct
     4081 gctcctccac gatggcccgc atcaccttga ccatggtctt gcggcgctgc tcgatggtct
     4141 ggatgagcca gcgtgccgaa ttgagcttcg acaccacgta atcgcgcgtt tcgccgttct
     4201 tgggggcgcc gtccttcaac acgtgctcgt aggccgagca gatgcgcagc cgcggcactt
     4261 tccggtcgtt caacgtaaag acatagtcct cgcccacccg ctcgacgatg agatccgggt
     4321 agacgtactg cgcgtcggca cctgaaacct ggttgcccgg caccggagaa agcgtgccga
     4381 tcaggtcgag ggcggtctgc acctcctcca cgggcacctt gagcgagcgg gcgatgtcga
     4441 tctgccgccg ccgcaccagg tcgtcgaagt gcttgtccac gatgcgccag gagagctccc
     4501 cctcctggcc gcgggcccgg agctggagga gcaggcattc gcgcaggtcg cgcgcgccga
     4561 tgcccggagg gtcgagggcc tggatggcct tcagcaccga ctcgatggtc tccaccggct
     4621 tgtccagctc ggccgccact tcctcgacgg tcatggtgag gtagccacgt tcgtcgatgg
     4681 agccgatcag gtagtcgccg atggcctcgg cctcgtcccc cattcccttg tagcggagct
     4741 gttcggcgag gtgctcatcc agcgacgggc gagaaatggg tacgcgctcg acgaattcct
     4801 cgtgccgctc ctcggaccgg gtggtggagg cgtcggaccc ctgctgcatg aactcggtcc
     4861 agtccacttc ctcgggcgcc tcgggggctt cgaggggctc ctgttcctcg cgctccaggc
     4921 gtccttcctc gccgaaatca tcctcggcgg tggctttctc gggctccggc tcctcggtga
     4981 tctcgtcgat ctcctcgagg agcggattgg agaccatttc ggccttcagc atctgctgga
     5041 gctcgagagt gggcgcctgc agcagcttga gcgcctgctg gagcttcggc gtcatgacga
     5101 gcttctgcgt ctgcgtgagg cgcaggccct gcctcatctc catctatgtg gtcctttcac
     5161 ggaacgtcgt cggttggcgg ttcatagttg gaatttctcg ccgaggtaga ggcgacgcgc
     5221 ttccggatcg gcggcgagtt gatcggccgt ccccgaaagc atgatgcgtc cctggaacat
     5281 gatgaacgcg tgatccgtgg atgacaaggt ctcacggacg ttgtgatcgg tgatcaggat
     5341 gccgagcccc ttggccttga gcctcaacac gatctcctgg atctccgcca ctgcgatcgg
     5401 atcgatcccg acaaatggct cgtcgagcag cagaaagctc ggtcgcgtgg cgagcgcccg
     5461 cgtgatctcg aggcgacgtc gctcgccccc cgacaggttc cacgccttgc gatcggccag
     5521 ccgcgtcatg tcgagctcgg ccaggagctc gtcgagccgc gcacttcgat cccccttcga
     5581 catgggaatc gtctcgagga tggcctggat gttgtccttg accgacagct tgcggaagac
     5641 cgacggctcc tgcggcaggt aacccacgcc gaggcgcgcg cgccggtaca tcggcatctg
     5701 cgtgatgtcg gtatcgccga tgcggatcgt cccggctttc ggcttgagga gccccacgat
     5761 catgtagaaa gtggtcgtct tccctgcacc gttcgggccg aggagtccca ccacctgccc
     5821 cggttccacc gacaggctca cgccgtccac gaccttgaac ttgccgtagt agcgcacgag
     5881 cccttcggcc atgaggtgcg gaccggggag tatggcgggc tcggtggggc gcgtcatctc
     5941 agcggggcgc gtcatcggtt gcgtcacggg catcgatttt cacctgttgg gtggtgtaat
     6001 gcgtcaaatc gggatcgctc tcgaacccca cgccggtaaa cacaccgtcc ggccggccca
     6061 gccggaacgg caggtcggtc ttcacacggt tctgcacgct gctccagtcc agccgctcgg
     6121 tcgtaagcgt atcgcctttc gttgtgatga caaccacgtg gccccgggcg gtcatgtcgt
     6181 tgttgctgcg ctgcacgacc ccttcgtcgg cggtcaggaa cgaggagggc tggccggcct
     6241 cgtcgtagaa atcgacgtgg atgttcgagg ccaccgtccg gttcggttgg gtgaaagttt
     6301 ccgaggagtc ggccttcagc gcccagcgga gccggccgtc cagtgtctcg cgcagcgtga
     6361 cgccgctcag catcgagctc ggttcgccga ccgccggtgg cgcggtgttg ccgtcgggtt
     6421 ttgaaccgca cgactggacg cccgtcgaca ggatggccag tgccgtccac atcgccacca
     6481 tgcgtgccgc ggaggagagt tcgcgcgcga cggacgcggc ggccccggcc ggcacttgtg
     6541 acggacggcc ccagcgcgga tgcatccagg cccacgaggc ggggtcgatg cggatcatgg
     6601 cttccagcgc gcgaacggcg gcttcgagca gggagtcggc gcgcgaggcc ggcgagccgg
     6661 tggcagccga cgcgagcgcc gcgccgacga ccacccgatg accgccatcc gaacgacgcc
     6721 ggatgacgat cgggagcatc gaggcgcccg tggcgatcgc gagacgcgcc ggacccgtgg
     6781 cgaaacgcga agggcggccg aagaagtcga tccaccgtcc cccggaacgg acgcgatgat
     6841 cgatgaggag gccgagcacc ccacccgcgg tcaacacgcg atgcgcctcg cgcgcgccgc
     6901 tcatggtgcc caccttgatg gtgcggcttc ccaggctcgc gcgatgcgca tcgagccagg
     6961 cgtcgagccg cgcctcgcgc accggcttgg aaaggccgtg aacgg
//
LOCUS       DASVOD010000725         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_465913, whole genome shotgun sequence.
ACCESSION   DASVOD010000725 DASVOD010000000
VERSION     DASVOD010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465913"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VF720_15655"
     CDS             <1..889
                     /locus_tag="VF720_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880848.1"
                     /translation="ATNDVWTLTNANGSGGTPVWSPATPTGTPPSPRRRFAFHYDATA
                     KRALVYGGDDPCGAPSGDVFVLNNADGTGGTPAWTSLPLSNGGAPAGSGIAGTYDGTT
                     NRLLALGGRGADGTTLLPDVVIIEKANGVSAGPNWTLLPPPSTAPPARERSAVAFEAP
                     GQRLMAFGGLGASGPLNDTWVLQLTSASGTVVAVPEPVGQRPGPGLAVGAVAPNPSRG
                     ALGFTVELDRVMAITGTLFDVGGRRVASVLDEELRAGAHRFAWDSRRSGGSPPASGTY
                     FLVIEGEGARQVRRVTIVR"
     gene            complement(943..>1629)
                     /locus_tag="VF720_15660"
     CDS             complement(943..>1629)
                     /locus_tag="VF720_15660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7880849.1"
                     /translation="PCGDRTTEGAVLTVTSPPTISSHPGPASINPPAMAPVSVTADGP
                     VSLYQWQIEASPGVWLDFTTKPTDLPCGGSAAADVDNSANVQIGITPCPGVPEYQVRV
                     VVSNACGSDTSNEATLTVTPVGGVPGNGAGASALLAKPAPNPTHGSTTLGYTLPASSP
                     VLLEIFDAAGRLVKVLVAEEQSAGRHEPVWQGTDASGRAVLSGIYFARLRTFHNGRPV
                     ESQRKINLVR"
BASE COUNT          241 a          560 c          577 g          251 t
ORIGIN      
        1 ggccacgaac gacgtctgga ccctgacgaa cgccaacggc tccggcggca ccccggtctg
       61 gagtcccgcc acacccaccg gcacgcctcc gtcaccccgg cggcgctttg cgtttcacta
      121 tgacgccacg gcgaagcggg cgctggttta cgggggggat gatccctgcg gcgctccgtc
      181 cggcgatgtc ttcgtgctga acaatgccga cgggaccggc ggcaccccgg cgtggacgag
      241 ccttccgctt tcgaacggag gggctccggc cgggagcgga attgccggca cctacgacgg
      301 caccacgaac cggctgctgg cgctcggcgg acgcggggcc gacggcacga cgctcctgcc
      361 ggatgtcgtg atcatcgaga aggccaacgg tgtttccgcg gggccgaact ggacgctgct
      421 gccacccccc tccacggcgc ctcccgcgcg cgagcggtcg gccgtggcgt tcgaagcgcc
      481 gggccagcgt ctcatggcgt tcggagggct cggcgccagc ggacccttga atgacacctg
      541 ggtgctgcag ctgaccagcg ccagcggcac ggtcgtggcg gtgccggagc ccgtcggcca
      601 acgtcccgga ccggggctgg ccgtgggcgc ggtggcaccg aacccgagcc gcggggcgct
      661 gggcttcacg gtcgaactgg atcgtgtcat ggcgatcacg ggcacgctct tcgacgtcgg
      721 cggccgacgc gtggcatcgg tcctcgatga ggagttgcgt gccggcgccc accgatttgc
      781 gtgggattcc cgccgatccg gcggctcacc gccagcctcc ggcacctact tcctggtgat
      841 cgagggagag ggcgcgcggc aggtccgccg ggtgacgatc gttcgctgat tcaatccgga
      901 tgatctcttg cccttcggga cggtgacggc ggccgacgcc gctcagcgca ccaggttgat
      961 cttgcgctgc gattccaccg gccttccgtt gtggaacgtg cgaagccggg cgaagtagat
     1021 ccctgacagc acggcccgcc cgcttgcgtc ggttccctgc cagaccggct cgtgacgacc
     1081 cgcggattgc tcttccgcga cgaggacttt caccagcctg cccgcggcat cgaagatttc
     1141 cagcagcacg ggggaggaag cgggcagcgt gtagcccaac gtcgtgctcc cgtgcgtcgg
     1201 gttcggcgca ggcttcgcaa ggagcgcact ggcgcccgcc ccgttcccgg gaacgcctcc
     1261 caccggcgtg acggtgaggg tggcctcatt gctggtgtca ctgccgcagg cattggagac
     1321 caccacccgc acctggtact cgggcactcc ggggcacggt gtaatcccga tctggacatt
     1381 ggccgagttg tccacgtccg ccgccgccga gccgccgcag ggaagatcgg tgggcttggt
     1441 ggtgaaatca agccacacgc cgggcgacgc ctcgatctgc cactgataca aggacacagg
     1501 accatcggcg gtgaccgaaa caggagccat ggccggcggg ttgatggacg ccgggccggg
     1561 atggctcgaa atcgtcgggg gcgacgtgac ggtgagcacc gccccttcgg tcgtgcgatc
     1621 gccgcaggg
//
LOCUS       DASVOD010000726         1673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_154501, whole genome shotgun sequence.
ACCESSION   DASVOD010000726 DASVOD010000000
VERSION     DASVOD010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154501"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF720_15665"
     CDS             <1..260
                     /locus_tag="VF720_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880850.1"
                     /translation="TIEVPLPKAAFWVTYVPNDPGETPPTIELRGNGSCTTGNGVQAM
                     RVVEGEYAKYCHADDGARFSIAHGQTGADTVYGTLALELKR"
     gene            772..894
                     /locus_tag="VF720_15670"
                     /pseudo
     CDS             772..894
                     /locus_tag="VF720_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1064..>1673
                     /locus_tag="VF720_15675"
     CDS             1064..>1673
                     /locus_tag="VF720_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880851.1"
                     /translation="MKRLLVLFALAVAGCSDAARIGNPLASPSAPAMATAPAGVPAPP
                     AGFTLTWSDDFTGAANTGLNTTNWKYDVAAGSTYGTGEIESTTSSTANVYQDGTGNLV
                     IKAIHTGTNPTSGWTSGRIETQRADFGAPAGGVLLIQSSIQQPDANLTTANGLGYWPA
                     FWMLGAALRSGGTWPSVGEVDILEDVNAQSVVYGTLHCGTSPG"
BASE COUNT          311 a          590 c          540 g          232 t
ORIGIN      
        1 acaccatcga ggtgccgctg cccaaagcgg cgttctgggt gacgtacgtg ccgaatgatc
       61 ccggagagac gccgccgacc atcgagctgc gcggcaacgg atcctgtacc accggcaacg
      121 gcgtccaagc gatgcgagtg gtcgagggag aatacgccaa gtattgtcac gcggatgatg
      181 gcgccagatt ctcgatcgcg cacggccaga ctggcgcgga cacggtctac ggaaccctcg
      241 cgctcgaact gaaacggtga gagcggcatg aggcgaccgg ccaagcctgg gcacgtcggc
      301 aggatccatc cgccacgcac gtcgaccccg catgcacgac gcgcgcagca ctaccgccac
      361 catgacgtga cccccggaag ctgatccgcg gcgccaatgt gtccgggcgg gagagaagcg
      421 ccacctcgcc gacgagcaaa agcccgtcac caggcgcgaa caggtgaacg ccgccacctg
      481 gccgatcgac gggcacgccg tcgagcaccg ccgcgcgcac caccgaattg cgcgttggct
      541 tgagggcgag gcgaccgccg acggacgtga aagggaaagt cgacggtccc tccactccgc
      601 tcgaggcgaa ttccgcgccc atccctgggg atccgtgtag aggaagaact ccgcgccctt
      661 ccatccgacg agcggacgaa gatcgagggt gaattgtgcg gcggcagcgc cgagtcctac
      721 agcgccgcgc tgtgcgccgc cgtcgacgtc gttcacctcg gcgccggtgt acatcaggtt
      781 cgtcgactcg gcaagccgga agaaggtgcc ccgctcgtct gcttcctctc ggacgatgcg
      841 tcgttcatga cgggcggcta ccacctcgtc gacggcggct acaccgacgt atgagcgcgg
      901 ccgagcgcgc gcgccgcttc gcccacacgg ccccctccct tcctgacttc ctggacacgg
      961 gaggtcagcc cagccacgtt ctagccgagc cgtcgcggcg ccttagccga agccggtcaa
     1021 gacgcgcgtc gtcggcccgg ttggaggaac catgggaggt cccatgaaac gccttctcgt
     1081 gctgttcgcg ctcgcagtcg ccggttgttc agatgccgcc cggatcggga atccgctagc
     1141 cagccccagt gcccccgcca tggcgacggc ccccgccggc gtaccggcgc cgccggcggg
     1201 attcacgctc acgtggagcg acgacttcac cggggcagcc aacactggcc tcaacacgac
     1261 caactggaag tacgacgtcg ccgccggcag cacgtatggt accggcgaga tcgaaagcac
     1321 gacgagcagc accgcgaacg tctatcagga cggaaccggg aacctcgtca tcaaggcgat
     1381 ccacaccggc accaacccca cgagcggttg gacctccgga cgtatcgaga cgcaacgcgc
     1441 cgactttggc gcacctgccg gcggcgtcct cctgatccag tcgtcgatcc agcagcccga
     1501 cgcaaacctc accaccgcga acgggctcgg ttactggccc gcgttctgga tgctcggggc
     1561 cgcgctgcgc agcggcggca cgtggccgag tgtaggagag gtcgacatcc tcgaggacgt
     1621 caacgcccag tcggtcgtct atgggacgtt gcactgtggc acatcgccgg gcg
//
LOCUS       DASVOD010000727         4986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_830256, whole genome shotgun sequence.
ACCESSION   DASVOD010000727 DASVOD010000000
VERSION     DASVOD010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830256"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..1044)
                     /locus_tag="VF720_15680"
     CDS             complement(196..1044)
                     /locus_tag="VF720_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7880852.1"
                     /translation="MSPDDIAVGTPGSVYIHGTAPEEQSRLTAMNRMINGRCLAALRL
                     RPGESVLDVGSGLGQMTREMARVTGRRVIGIEASPAQREEAIRQATADGESDLVEFRE
                     GDAMNPPLRQEEWGAFDVTHTRFLLEHVTDPGAVVAAMLRATRPGGRLLLLDDIHSLL
                     RLWPTVPGLSEVWLAYQRSYDRIGCDAAIGERLVEILHRAGAQPVRNELVFFGSCSGD
                     PFFPTIVENLAVILEGARTNIVDAGYIPAADVDRVAAELRRWGRRPDAAFWYAMGMAE
                     GRRPSS"
     gene            complement(1041..3953)
                     /locus_tag="VF720_15685"
     CDS             complement(1041..3953)
                     /locus_tag="VF720_15685"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX7880853.1"
                     /translation="MTCRPSPRWFAVALAAFLAVGVAHAQSPKVDSDNLKLKPPAVQV
                     GPAPDLSTPLPLDPSVRTGTLPNGMTYYIKSNGWPENRVSLRLAVNAGSVLESDEQQG
                     FAHFLEHMNFNGSKHFESGELVKYLESIGARFGADANAYTSFDETVYMLDLPADQDSL
                     TIKGLWAMGDYAGRARLSDKEIEKERGVVLDEWRRGRGAQARLNDKHFPVLFAGSEYA
                     KRLPIGKPEVIEHGAPGLIRDFYAKWYRPERMAIIAVGALDPDSMLARIQAEFSDIPK
                     SESLAPLPVHEVPAHEDLKVSVATDAEARFTNASLYFKKPHELKKTLGDYRDQLTDWL
                     VYSMLGDRFDEQGRRADAKFLNAFAGGFSYVRTMDVLFLNVRTEDSSLIPGLEAAITE
                     LKRAGQHGFGQAELDRARESFMAQVERSYQERSRTESQGLASEYVSHYLDHEPAPGIE
                     NEYEYARSLLPGITLDDVNGAMTRALASNSRVLTVSAPQKEGLTPPAEADLRAAVERA
                     WQAPVEAWVDVVADRPLMAEPPKPGTVTATRTIPELGATVVTLSNGAEVWMKPTDFKN
                     DQILFSCYAKGGSSIAEPDDYPEASRASLIIGEEGIGGFKPVELEKLLAGKLVYGQPY
                     VTSYTHGLSGASTPKDLETGMQLLYLTFTMPTDRPEAFDVLRKKWHSDMENRANDPDA
                     LFGDTVGKLNSMDHYMSRPLTAADVDKLDFSKAHQFYKDRYANAADFTFFFVGAFDPA
                     VMTDLATRYIGALPSTGKKTSAPVDRRYGFPMGIQTAGLAKGVEPKSRTQITFYSPTG
                     VDEMEMYRARMAGNILRRRLRETLREELGTTYSVGVSFSNPSLVTNWGTLTVSYSGAP
                     ENADRMADTTLAIVKRLVAEGPHADEVASLQEMERRELETAEKQNAYWLGSLQLVHQL
                     GFDPLRVAKRRERIDTLTAESLHDAFRKYLPVDRYTRVTLMPESTPGGDIAKPARP"
     gene            4178..>4986
                     /locus_tag="VF720_15690"
     CDS             4178..>4986
                     /locus_tag="VF720_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX7880854.1"
                     /translation="MFEQFFGLSENPFSLTPDPRFIYASKSHDEALAHLKYWLEHKEG
                     FALITGEVGTGKTTALFRLIDGLERRYEIAFITNSTLSPVDLLEEICRKFGMEVEQGT
                     SKPALLQKLEAYLRRQSDRGNGSLLIIDEAQNFEQPLLEEVRLLSNIAKPGGPPLLQI
                     ALIGQPELERKLSLPELRQLKQRIGIHYRIEPLNHEETIRYVHHRVGVAGGYPKVLFA
                     EETVSILYDRTHGLPREINQVASQALLAAYVEDSTRVRPEHIAGAIKEMSFR"
BASE COUNT          837 a         1581 c         1690 g          878 t
ORIGIN      
        1 ttaccgggaa aaaagtgctc gtgattggcg gcgagcgcca ggacaacggc ggaacagaca
       61 acgaggctcg cgggagtgag ccatcggccg gggacgcgca tgggcaattc tccgtcggat
      121 caatagggcg gccaagtgag cgggagaacg atgacaagag tatagcccag cgcccaggcg
      181 gccccggacg gggaatcagg aggagggacg ccgcccttcc gccattccca tggcgtacca
      241 gaaggcggcg tcgggtcggc ggccccagcg acgcaactcg gccgcgacgc gatcgacgtc
      301 ggcagccggt atgtaacccg catccacgat gttggttcgc gccccctcga ggatcaccgc
      361 aagattctcg acgatggtcg ggaaaaaagg atccccggag caggagccga agaagaccag
      421 ctcgttccgg accggctggg cgccggcccg gtggaggatt tccaccaggc gttcgccgat
      481 ggcggcgtcg cagccgatgc ggtcgtagct ccgctggtag gccagccaca cctcggaaag
      541 gcccggcacg gtgggccaga gccggagcag cgagtggatg tcgtcgagca gcaggaggcg
      601 accgccggga cgggtggcgc ggagcatggc cgccacgacg gcgccggggt cggtgacgtg
      661 ctcgaggagg aagcgggtgt gtgtgacatc gaacgcgccc cactcctcct ggcggagcgg
      721 cgggttcatc gcgtccccct cccggaattc caccagatct gattcaccat cggcggttgc
      781 ctgccggatg gcttcctccc gctgggccgg gctggcctcg atgccgatga cccgccggcc
      841 ggtgacacgg gccatttccc gcgtcatctg gccgaggccc gatccgacgt cgagcaccga
      901 ttcaccgggc cgcagccgga gcgccgccag gcaccggccg ttgatcatcc ggttcatggc
      961 tgtcaggcgc gactgttcct cgggcgcggt cccatgaatg tacacggacc cgggcgtccc
     1021 gaccgcgatg tcgtccgggc tcacggcctg gcgggcttgg cgatgtcgcc tcccggcgtg
     1081 gattccggca tcagcgtgac gcgcgtgtag cgatcgacgg gcaggtactt ccggaaggcg
     1141 tcgtgcaggc tttccgccgt cagcgtgtcg attcgttcgc gccgctttgc cacccggagc
     1201 gggtcgaagc cgagttggtg aacgagctgg aggctcccca gccaataggc attctgcttt
     1261 tcggcggttt cgagctcgcg ccgctccatc tcctggaggc tggccacttc gtccgcgtgt
     1321 ggcccctcgg ccaccaggcg cttcacgatg gccagcgtgg tatcggccat ccggtcggcg
     1381 ttctccgggg cgccggagta cgacacggtg agcgtgcccc agttggtcac gagcgagggg
     1441 ttcgagaagg agacgcccac gctgtaggtc gtccccagct cctcacgcag tgtttcacgc
     1501 aggcgacgcc ggaggatgtt gccggccatg cgggcccggt acatttccat ctcatcgacg
     1561 ccggtggggc tgtagaaggt gatctgcgtc cggctcttcg gctcaacccc tttggcaagg
     1621 ccggccgtct ggattcccat cgggaaaccg tagcgccggt ccaccggcgc gctggtcttc
     1681 ttcccggtcg acggaagcgc gccgatgtag cgggtggcca ggtcggtcat cacggccgga
     1741 tcgaacgcgc ccacgaaaaa gaaggtgaag tcggcggcgt tggcgtagcg gtccttgtag
     1801 aactggtgcg ccttgctgaa atcgagcttg tccacgtcag cggcggtcag tgggcgcgac
     1861 atgtagtggt ccatgctgtt gagcttcccc accgtgtcac cgaagagggc atcgggatcg
     1921 ttggcccggt tctccatgtc ggagtgccac ttcttccgaa gcacgtcgaa cgcctccggc
     1981 cggtcggtcg gcatggtgaa cgtgaggtag aggagctgca tgccggtctc gagatccttc
     2041 ggcgtgctgg cccccgaaag gccgtgggtg tacgaggtga catagggttg cccgtacacc
     2101 agcttgccgg ccaggagctt ttccagttca accggcttga agccaccgat cccttcctcg
     2161 ccgatgatca acgaggcgcg gctcgcctcc gggtaatcgt caggttcggc gatcgacgac
     2221 cctcccttgg cgtagcacga gaagaggatc tggtcgttct tgaagtcggt cggcttcatc
     2281 cagacctcgg cgccgttcga cagcgtgaca acggtggccc cgagctcggg aatggtgcgc
     2341 gtggcggtca cggtgccggg cttcggcggt tcagccatga gcggccggtc ggccaccacg
     2401 tccacccagg cttcgaccgg cgcctgccag gcgcgttcga ccgcggcccg aagatcggcc
     2461 tcggccggcg gggtcaggcc ttccttctgc ggcgccgaca cggtgaggac gcggctgttc
     2521 gaggcgaggg cgcgggtcat cgccccgttc acgtcgtcga gcgtgatgcc ggggaggagt
     2581 gacctggcgt actcatactc gttttcgatt cccggggcag gctcgtggtc gagatagtga
     2641 cttacgtatt cggaggccaa tccctggctc tcggtccggc tcctctcctg gtacgagcgc
     2701 tcgacctggg ccatgaacga ttcccgggcg cggtcgagtt cggcctggcc gaagccgtgc
     2761 tgcccggccc gcttcaattc cgtgatcgcc gcctcgaggc cgggaatgag gctggagtct
     2821 tccgtgcgga cgttgaggaa caggacatcc atcgtcctga cgtagctgaa gccgccggca
     2881 aacgcattga ggaacttcgc atcggcccgc cgtccctgct cgtcgaagcg gtcgcccagc
     2941 atcgagtaga caagccaatc ggtcagctgg tcgcggtagt cgcccagcgt cttcttgagc
     3001 tcgtgcggct tcttgaagta aagggaggca ttggtgaacc gggcttcggc atcggtggcc
     3061 accgagacct tgaggtcctc gtgggccgga acttcgtgca ccggcagcgg cgcgagtgat
     3121 tcgctcttcg ggatgtcgct gaactccgcc tggatcctgg ccagcatcga atcgggatcg
     3181 agcgcgccga cggcgatgat ggccatccgc tcggggcggt accacttggc gtaaaagtcg
     3241 cggatgaggc cgggcgcgcc gtgctcgatg acttccggct tgccgattgg aagcctcttt
     3301 gcgtactcgg agccggcgaa gagcaccggg aagtgcttgt cgttgaggcg ggcctgcgcc
     3361 ccgcgcccgc gccgccactc gtcgagcacc acgccgcgct ccttctcgat ctccttgtcg
     3421 gaaaggcgcg cgcggccggc gtaatcgccc atggcccaca gccccttgat ggtgaggctg
     3481 tcctggtcgg ccggcaggtc gagcatgtac accgtttcat cgaacgacgt ataggcgttg
     3541 gcatccgcgc cgaagcgggc gccgatcgac tcgaggtact tgaccagttc acccgattcg
     3601 aaatgcttgc tgccgttgaa attcatgtgc tcgaggaagt gggcgaaccc ctgctgttca
     3661 tcggattcga gcaccgatcc cgcgttgact gccagtcgaa gcgacacccg gttctcgggc
     3721 cacccgttcg acttgatgta gtaggtcatc ccgttgggaa gcgtgcccgt cctcaccgag
     3781 gggtcgagcg gcaggggagt ggaaaggtcg ggggccggtc ccacctgcac ggcgggcggc
     3841 ttgagcttca ggttgtcgga atcgaccttc ggactctgcg cgtgggcgac gccgacggcc
     3901 aggaaggccg cgagcgccac ggcgaaccag cggggggatg gacggcaggt catgggctct
     3961 ctccttggcg gaacacggga gggatccgcg gggggcggac atctcaccta tagtagtgga
     4021 gaattggctg aagtggcccg gggccgaggc cgacaagttg acccgggtaa agcggcccga
     4081 ctaaactcca cggtcgattt ctcgacagcg ggggattgta cagtaaacgg ttccgccggt
     4141 ttcagttatc gccgaaaccc ggatcccatg aggtgtcatg ttcgagcagt tcttcggtct
     4201 cagcgaaaac cccttcagcc tcacgccgga cccccgtttc atctatgcca gcaagagcca
     4261 cgacgaagcc ctggcccacc tcaagtactg gctggagcac aaggaggggt tcgccctgat
     4321 cacgggcgaa gtcggcacgg gtaagacgac ggcgctcttc cgtttgattg atggcctcga
     4381 gcggcgatac gaaatcgcct tcatcaccaa ttccacgctc tcgccggtcg acctcctgga
     4441 ggagatctgc cggaagttcg gcatggaggt cgagcaggga accagcaaac ccgcgctgct
     4501 ccagaagttg gaggcctacc tccgtcgcca gtcggatcgg ggaaacggct ccctcctgat
     4561 catcgacgaa gcccagaatt tcgagcagcc cctcttggag gaggtgcgcc tcctctccaa
     4621 catcgccaag cccggcggac cgccgcttct ccagatcgcc ctgatcggcc agcccgagct
     4681 cgagcgcaag ctcagcctgc cggaactgcg gcagttgaag cagcggatcg gcattcatta
     4741 ccgcatcgaa ccgctgaacc acgaggagac gatccgttac gtgcatcacc gggtgggcgt
     4801 ggccggtggg taccccaagg tgctgttcgc cgaggagacc gtgtcgatcc tctacgacag
     4861 gacccacggg cttccccgcg agatcaacca ggtggccagc caggcgctcc tggccgcgta
     4921 cgtggaagat tccacgcggg tgcgtcccga gcacatcgcg ggtgccatca aggagatgag
     4981 tttccg
//
LOCUS       DASVOD010000728         3844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_204501, whole genome shotgun sequence.
ACCESSION   DASVOD010000728 DASVOD010000000
VERSION     DASVOD010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204501"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="VF720_15695"
     CDS             complement(<1..805)
                     /locus_tag="VF720_15695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880855.1"
                     /translation="MRRPIEAVAVPLVFAVLAAAAPAHADIHLSSVPSHVYKIADPGR
                     LRTESWFFNVLVQDDAKREGVKTTRAVMEVYAGDRLRETVTLPDSTVIRMRGMNYRMP
                     PNADANTPQRPLLLDNVFDIRLAFAARPIAWKADRVRVTLTFDTPDGGPVTRTLDVAL
                     GTYVQKTELLFPLEKPAIVTQGQINNSGHAGHSNQFAIDIMGLNAEYGPMVTGKTDNE
                     EFAGWGMNILAPAAGSIVYARNDVPDCPADGEPYDIYSKLPDPVQASAGN"
     gene            complement(903..1514)
                     /locus_tag="VF720_15700"
     CDS             complement(903..1514)
                     /locus_tag="VF720_15700"
                     /inference="COORDINATES: protein motif:HMM:NF044334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880856.1"
                     /translation="MIRFLVLAELIVAALTVFLGVLSWLLLVFLPSVIPAVVLGLAAM
                     VRPTLVLGRRRSRNTLHIASVVNTAWCVMYMVSVVTNIAEPARFIVPNGYTGSVAVVF
                     DRPWGLEPEYDGRRRIYRIPDDGVLATRLEFDNGWQFSPVEYVDRTGVTVRTRGLIVG
                     SGGGVLGGACTYSFEEFWVEPVPSKARSVRDSEAFHRAACRAP"
     gene            complement(1546..2619)
                     /locus_tag="VF720_15705"
     CDS             complement(1546..2619)
                     /locus_tag="VF720_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880857.1"
                     /translation="MNGRRGAMSTVILSLVILATLALVTACQRATPPAGKPAPVSEVA
                     LARSPDAAPRFVSGGTDRIVGLLCGATLHRFVAGNEALSPSSAPAVDIRAAWFGPDPI
                     IDRWLVRTNDGIAVTTRDLAVPMPEWPAGADVGPAVFASGGATLAAAPAGGDDRRVRV
                     CNFEPSKVQPGFVADNPLILPVAEGATVLFVDLDLTGKYILTVDDQGHIGVYQAPGTK
                     PVYTQTLDPETEGTIRAADLSPEGDWFVVSARSVKVRAWRLPFMSAPLTSFGDVTQVF
                     FAGGVESLITVDENGLAIRWLLNYGKVKAEATAQLDSLTTAVTADASGRLLMSLTPDG
                     RVRAWPTATLAELEALTQRVCVR"
     gene            complement(2834..>3844)
                     /locus_tag="VF720_15710"
     CDS             complement(2834..>3844)
                     /locus_tag="VF720_15710"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010608346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX7880858.1"
                     /translation="ISRPGSEKIIRYAFEYAKRNNRKKVTCFTKDNIMKMSDGLFHRI
                     FDEIAAEYPDIVNEHWIVDIGAAKLADTPEAFDVIVMPNLYGDILSDVAAQIAGSVGL
                     AGSANIGTGAAMFEAIHGSAPRRAGQNLANPSGLLLGGVLMLVHIGQTDVAEKVHNAW
                     LKTIEDGVHTYDIYDESVSKEKVGTKEFAQAVIARLGQKPATLKAVSYVAGEPMVIVP
                     AKRKESARELVGVDLFLHWDKGTASDLAGALNAATADGLVLKTVSNRGQKVWPDGLPE
                     TFTTDLWGCRFQASKAGSSIQPRQIVALLSRVAEAGLDCVKTESLYTYDGKPGYSLGQ
                     GE"
BASE COUNT          670 a         1311 c         1221 g          642 t
ORIGIN      
        1 aattcccggc cgaggcctgg accgggtcgg gaagcttcga gtagatgtcg tacggctcac
       61 cgtcggccgg gcagtcgggc acatcattgc gcgcgtacac gatgctcccc gccgcgggcg
      121 ccaggatgtt catcccccac ccggcaaact cctcgttgtc ggtcttcccg gtcaccatcg
      181 gaccgtattc cgcattcagg cccatgatat cgatcgcaaa ctggttcgag tgccccgcgt
      241 gccccgaatt gttgatctga ccctgcgtga cgatggccgg cttctcgagc ggaaacagta
      301 gttccgtctt ctgcacatag gtcccgagcg cgacgtccag cgtccgggtg accggcccac
      361 cgtccggcgt gtcgaacgtc agcgtcaccc tcacgcggtc ggccttccac gcgatgggcc
      421 gggccgcgaa cgccagccgg atgtcgaata cattgtcgag gagcagcgga cgctgcggcg
      481 tgttcgcgtc ggcgttgggc ggcatccggt agttcatccc tctcatccgg atcaccgtgc
      541 tgtcaggcag cgtcaccgtt tcccggagcc gatcgcccgc atacacctcc atcaccgccc
      601 gggtcgtctt cactccctcg cgcttcgcgt catcctgcac cagaacattg aagaaccagc
      661 tctcggtgcg caggcgaccg gggtcggcga tcttgtagac gtgggagggg acggaggaga
      721 ggtggatgtc ggcgtgggcg ggcgcggcgg cggcaagaac ggcgaaaacc aggggcacgg
      781 ccacggcctc gatggggcga cgcatgtcgg atcctccgga ggggacggat ggagcggggc
      841 cctgttgggc acaggcctaa gacggcagcc ggggcggatt tgttcgaagg gggagctatg
      901 gatcagggcg cacggcaggc cgcacgatgg aaggcctcgg agtcgcgtac agatcgagcc
      961 tttgatggaa cgggctcaac ccagaattcc tcgaaactgt aggtgcaggc cccgcccagt
     1021 acgccccctc ccgatccaac gatcagaccg cgtgttcgca ccgtcacacc ggtgcgatcg
     1081 acgtactcga ccggggagaa ttgccaccca ttatcgaatt ccaaccgcgt ggccaggacg
     1141 ccatcgtcgg ggattcgata gatgcggcgc cgtccatcgt actcgggctc caacccccag
     1201 ggcctgtcaa ataccacggc taccgatccc gtatatccgt tgggcacgat gaatcgcgcc
     1261 ggttccgcga tgttcgtgac gacggagacc atgtacatca cacaccaggc cgtattcact
     1321 acggacgcga tgtgcagtgt attgcgcgac ctgcgacgcc cgagtacgag tgttggtcgg
     1381 accatggccg ccaatccgag cacgacggcg gggatcaccg agggaaggaa gacgaggagc
     1441 aaccatgaga ggactccaag gaacaccgtc agtgccgcga cgatgagctc cgcgagcacc
     1501 aggaaccgga tcatggtgag gaccgtgcga ccgggtgtcc ggtcactagc gtacgcacac
     1561 ccgctgcgtc aacgcctcca gctccgccag cgtcgccgtc ggccacgccc gcacgcgccc
     1621 atcgggcgtc agcgacatga gcagccgccc cgaggcatcg gccgtgaccg ccgtcgtcag
     1681 gctgtcgagc tgcgccgtcg cctcggcctt taccttgccg tagttcagca accagcggat
     1741 ggcgaggccg ttctcatcca ccgtgatgag cgactccacg ccgccggcga agaacacctg
     1801 cgtgacgtca ccgaagctgg tcaacggtgc cgacatgaac ggcagtcgcc acgcccgcac
     1861 tttgacgctc cgggcggaca ccacgaacca gtctccttcg ggcgacagat cggcggcccg
     1921 gatggtccct tcggtctccg ggtcgagcgt ctgcgtgtac accggcttcg tacccggagc
     1981 ctggtagacg ccgatatgcc cctggtcgtc cacggtcagg atgtattttc cggtcaggtc
     2041 caggtcaaca aacaagaccg tggcgccctc ggccaccggg agaatgagcg ggttatcggc
     2101 aacgaagccg ggctgcacct tcgacggctc gaaattacac acgcgcacgc gccgatcgtc
     2161 gccgccggcg ggagcggccg ccagcgtcgc cccgcccgag gcaaagaccg ccgggccgac
     2221 gtccgctccg gccggccact cgggcatcgg caccgccagg tcacgcgtcg tcaccgcgat
     2281 cccgtcgttc gtccgcacca gccagcgatc gatgatcgga tccgggccga accaggccgc
     2341 ccggatgtcg acggcgggcg cgctactcgg cgacagggcc tcgttgccgg ccacgaaccg
     2401 gtggagcgtg gcgccgcaga ggagaccgac gattcggtcg gtgccgccag aaacgaagcg
     2461 cggtgcggcg tcgggcgatc gcgccagggc cacctcggag accggggccg gcttgccggc
     2521 cggcggcgtg gcgcgctggc aggcggtcac caacgcgagg gtggccagaa tgacgaggct
     2581 caatattact gtcgacatcg cgccgcgtcg tccgttcatg acgtccactc cctccgtgag
     2641 ataccactgc ctgaatcatc gtctgtctta caatgtaaga catcgcgcgc atgtagtagc
     2701 cgcgcccccc caacctgacc acgaccccag aaaaagtaac gccaactcgg tccgaaccga
     2761 gttggcgata ccagatttca atacagtctt ttcgacatcg tcgggacggg gcccgcgagc
     2821 ccgcgccgct cccctactcc ccctgcccca gcgaataccc cggcttcccg tcatacgtgt
     2881 acagactctc cgtcttgacg cagtcgagtc ccgcctcggc cacgcgcgac agcagcgcca
     2941 cgatctggcg cggctggatc gaggaacccg ccttggaggc ctggaagcgg caaccccaga
     3001 ggtcggtcgt gaacgtctcg ggcagtccgt ccggccaaac cttctggccg cgattcgaaa
     3061 ccgtcttcag caccagcccg tcggcggtgg cggcattcag cgcgccggcc aggtcggaag
     3121 ccgtcccctt gtcccagtgc aggaagaggt ccacgccaac cagttcgcgc gcgctctcct
     3181 tgcgcttcgc cggcacgatg accatcggct cgccggccac gtaactcacg gccttgagcg
     3241 tggcgggctt ctgacccagg cgcgcaatga cggcctgggc gaattccttc gtgcccacct
     3301 tctccttgct caccgactcg tcgtagatgt cgtaggtgtg cacgccgtcc tcgatcgtct
     3361 tgagccaggc gttgtgcacc ttctcggcca cgtcggtctg gccgatgtgc accagcatga
     3421 gcacgccgcc gaggagcaat cccgagggat tggccaggtt ctggccggcg cgccgcggtg
     3481 ccgagccgtg gatggcctcg aacatggcgg caccggtgcc gatgttggcc gagccggcca
     3541 ggccgaccga gccggcgatc tgggcggcca cgtccgacag gatgtcgcca tacagattcg
     3601 gcatcacgat cacgtcgaac gcttccggcg tgtccgccag cttggcggcg ccgatgtcca
     3661 cgatccagtg ctcgttcacg atgtccggat actcggccgc gatctcgtcg aagatcctgt
     3721 ggaagaggcc gtcggacatc ttcatgatgt tgtccttggt gaagcaggtc accttctttc
     3781 ggttgttccg cttcgcgtac tcgaaggcgt agcggatgat cttctcgctg cccggcctcg
     3841 agat
//
LOCUS       DASVOD010000729         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_933736, whole genome shotgun sequence.
ACCESSION   DASVOD010000729 DASVOD010000000
VERSION     DASVOD010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933736"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VF720_15715"
     CDS             complement(<1..686)
                     /locus_tag="VF720_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7880859.1"
                     /translation="MKRGDDVDDTDRRRFLARLAAGATACLAAESVFATVSSMAASPM
                     DSPTPSVLPAGRAILGATGIETAFVAQGTGSGGWNRTSEQVKLGDRGFERLIRHGLER
                     GQNFIDTADLYGSHGATRKALAGVPRGDYTLLTKLWPEPASWCTPSGGAKPEIDRFRK
                     ELGTDHLDVVLIHCVGTSNWASRYARVRDELWALKSAGMIRAVGVSCHDLGALQAAAS
                     DPWTEVILAR"
     gene            780..>1581
                     /locus_tag="VF720_15720"
     CDS             780..>1581
                     /locus_tag="VF720_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880860.1"
                     /translation="MDRRDWVVLALITIAGLILRLLLWKTQSIPSVDGTAYLRMAMQL
                     AGGPAVESVHQYGYPVLVRLAHFVIPDWVTAARVLALVTGVLFIPSLGWLSAAFVGNR
                     FLRCLPAIAAAATPLMVRYSLTTMTEMPYFLLMAIGLGLAARRRALGAGIVFGLAYAI
                     RPEGLLVAVVAAAFIALVNRRPKEAAALLAGTALIVAPYVATIGVTQGKWTLTPKSLN
                     IAPGTWEAAEEQAGESHAARSVAERVERYGGETARLYPKNAGDVLMQLG"
BASE COUNT          233 a          591 c          510 g          247 t
ORIGIN      
        1 atgcgcgcca ggatcacctc ggtccacgga tcgctggcgg cggcctgaag cgcacccagg
       61 tcgtggcacg acacgcccac ggcccggatc atgcccgccg acttgagcgc ccagagttca
      121 tcgcgcacgc gcgcgtaccg cgaggcccag ttcgatgtcc ccacgcagtg gatgagcacc
      181 acatcgagat ggtcggtgcc cagctccttc cggaagcggt cgatctcggg cttcgcgccc
      241 cccgacggcg tgcaccacga ggcaggctcg ggccagagct tggtcaggag cgtgtagtcc
      301 ccgcgcggca cgccggccaa cgcctttcgc gtggccccgt gcgatccgta cagatcggcg
      361 gtgtcgatga agttctgacc gcgctcgagg ccatgccgga tgagccgttc gaacccgcga
      421 tccccgagct tcacctgctc ggacgtgcgg ttccagccgc ccgagccggt tccctgggcc
      481 acgaaggctg tttcgatgcc ggtggctccc aggatggcgc gcccggcggg gagcacggag
      541 ggcgtgggtg agtccatcgg cgacgcggcc atcgatgaca cggtcgcaaa caccgactcc
      601 gcggccaggc aggcggtcgc tcccgccgcc agccgcgcca ggaaccggcg tcggtcggtg
      661 tcgtcaacgt catcgcctcg tttcatggcg cacacgatag ggcagttccg cccgtcatcg
      721 ccaccctgta ccctgcgcgc atggccgcac cgtccgccac ccacaccgaa ccccgagcga
      781 tggaccgccg cgactgggtc gtcctcgcac tgataacgat cgccgggctg atcctgcgcc
      841 tcctcctgtg gaagacccaa tcgatcccgt cggtggacgg caccgcttat ctccgcatgg
      901 ccatgcaact ggccggcggc cccgccgtcg agagcgtgca ccagtacggc tatccggtac
      961 tcgtccgcct cgcgcacttc gtcattcccg actgggtgac ggcggcccgt gtcctcgccc
     1021 tcgtcaccgg cgtgctcttc atcccgtcgc tcggctggct cagcgccgcg ttcgtcggca
     1081 atcgcttcct ccgatgcctg ccggcgatcg ccgcggccgc cacacccctc atggtgcgct
     1141 attcactgac cacgatgacg gagatgcctt acttcctcct catggcgatc ggactcggcc
     1201 tcgcggcccg ccgccgcgcg ctgggggccg gaatcgtctt cggcctggcg tacgccatcc
     1261 gccccgaagg attgctggtg gcggtggttg ccgcggcctt catcgcactg gtcaaccgcc
     1321 ggccaaaaga agccgccgcg ctcctggccg gcacggcgct catcgtcgcc ccgtacgtcg
     1381 ccacgatcgg cgtcacgcag gggaagtgga ccctcacgcc caagtcgctg aacatcgcgc
     1441 ccggcacgtg ggaggccgcc gaagaacagg cgggggagtc gcatgccgcc cggagcgtgg
     1501 ccgaacgggt ggagcgctac ggcggcgaaa ccgcgcgcct ctacccaaag aatgccggcg
     1561 acgttctcat gcagctgggt c
//
LOCUS       DASVOD010000730         6719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_155046, whole genome shotgun sequence.
ACCESSION   DASVOD010000730 DASVOD010000000
VERSION     DASVOD010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6719
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155046"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..611)
                     /locus_tag="VF720_15725"
     CDS             complement(129..611)
                     /locus_tag="VF720_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880861.1"
                     /translation="MARRETGEPTSEAQLGACIDNLDPTNRRLFRSVRTALRRRFPAA
                     NELAYDYGDAVVISYSPSNNGIDGILALRGSTDGVFLYFSQGPQLPDPKKLLQGSART
                     TRFIQLESASRLKNPDVEALIMAALGHAKTPFPTRGKGSLILKSSAAKKKRTPGKPAK
                     "
     gene            complement(640..807)
                     /locus_tag="VF720_15730"
     CDS             complement(640..807)
                     /locus_tag="VF720_15730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880862.1"
                     /translation="MSTEFVCIPRPITRSESADGGPIRYRVVHTAALWKNGERPKLNV
                     SVLEGDVGLSI"
     gene            complement(804..2702)
                     /locus_tag="VF720_15735"
     CDS             complement(804..2702)
                     /locus_tag="VF720_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX7880863.1"
                     /translation="MTISRRSFLRTSAALGMSLAWAGPACVSRVQWQERRDLYPQGVA
                     SGDPDAHSVILWTRRPFDEGTRQLLTVEVAGDEAFSRVIAHAEAPVSSAADWTSRVLV
                     GGLKPGTTYWYRFTDADGNGSRVGRTITAPAPDDPRTVNFAFVSCQDINEGKLNAFRR
                     MIYEDERAPDAERLDFVLHLGDFIYEVVMYPDELPTRLDRTIYEVVRIQDGHTVGKFH
                     IPLNVDGYRAIYKAYLTDPDLQDARARWPFVAIWDNHEFSWQGWQSIVKAGSFEQPGQ
                     SIKVAANQAWFEYIPARIAGRDELLETFDPPAVKDVPIEEFDSDGLGTEANNLAAINS
                     LRAYRTLRYGRNLDLIVTDQHSYRSADPFSDPSLGELGGNEFMGMFPEPLMQVLDGGR
                     AFGEGTPPSELRWNEAHVANPQRGAPPQTILGAEQKAWFKDQLTRSTAVWKIWGNSLG
                     TLEYRADPNNLPAGLTTEAWPPDTFANMGPADYGTAWRERGEICDLVRDNGITGFAIV
                     SGDRHSFWAGYAAAELPPGKFEPVGLSFVGGSLVSPGPMEGTSTGCRRTRRCERCSSW
                     TGKVTRNRTGHSTCCSGTVSARASSMRRAWISRRQRPFPTRTWRRTSSSSISAGTATP
                     GSACRARR"
     gene            complement(2828..3322)
                     /locus_tag="VF720_15740"
     CDS             complement(2828..3322)
                     /locus_tag="VF720_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880864.1"
                     /translation="MTLKRILMLLIAMSITPAQAADPVTAPGSTWQNQDQVIEQFRAD
                     ISASPTELLAEGLVLSSGEAARFWPMFEAFQKEQKAITEEQLKSVLQYRDVYKKLTDD
                     DALDYVNSILKRDQKLLDLRVKYLARFQEVVSPRIAARAIQLDRRLGLAGQMRFASQV
                     PLIP"
     gene            complement(3433..4410)
                     /locus_tag="VF720_15745"
     CDS             complement(3433..4410)
                     /locus_tag="VF720_15745"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX7880865.1"
                     /translation="MNPRTRNILFGILILLGAWVLLVERPYKDDIRGPSTRKGRVFKD
                     FDPTGATHAAITTAGNITNLEKDGGTWIVSEGRFRADQKAVEEMLARVDTMVIGSVAS
                     ENPAKQSTFGVDTSGVHVVIEGGGGANFWVGTSSPDFNGLFVRVDGKNEVYSVPQVTR
                     FNFERGQQTWRDKRVVPFPAEEIRSIVMTWADTTVTLTREGTDSLKTSTWSVSGNLPG
                     EASSPAKPNDARLIANTLGNLMADTFPTAQDTIPVAWEPLVYRFDLTAANGDHAVIEV
                     GPKLAKGQHAVRRVGDPAVVMIGPWRFTRLKKGYDQLREMAQPPAPTPQ"
     gene            complement(4411..6039)
                     /locus_tag="VF720_15750"
     CDS             complement(4411..6039)
                     /locus_tag="VF720_15750"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX7880866.1"
                     /translation="MPARKGLFGSGSSIGVVLLIVAILGLVNIIAVKRFARLDLTQNR
                     EYTVTKSTKAILAGLDDVVNVTVYFSKDLPPYLATLDRQVKDLLDEYKAFSGGKLRIQ
                     FEDPGSDPTKERSLQMIGIPKVQLNILEKEKAQVTNVYLGIAIQYADKSETIPIVQST
                     ENLEYDLTSAILKVTQAPPKVGLLTGHGEATLERGQMSGAGQALQKQYEVTNVDISGG
                     QPVPADIRTLIVARPTQRLGDRDRYEIDQFVMRGGRLIVLADGTQIVPQQLMAQPFDS
                     GLNELLSHYGARIGSDLLLDASHVSTAFSNGSFSFMMPYPFFPRILPDNLNRKHPVTS
                     TLQSIVTPWVSSLETTVPVDTTGAAPEGGVHAEILAKTTKQSFRLEGQTSINPQQRLA
                     PPNPSEMTSYPVAVSLTGTFASAFAGRPAPPPPDSAAAAMAPNPATVDRSPSTQIIVV
                     ADADFASDQFTSQYPENLTFFLNAVDWCTLGDQLIAIRSRANFSRPLKPVPDNARGPI
                     KLANMLVMPLLVGAWGLFRWYNRRRAQNMAARYR"
     gene            complement(6039..>6719)
                     /locus_tag="VF720_15755"
     CDS             complement(6039..>6719)
                     /locus_tag="VF720_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880867.1"
                     /translation="KSYFDSPIAYVYIAVFLVVVNWFFFRDFFLAEQASLRSLFGTMP
                     LYFLFFVPAVAMRLWSEERKMGTVELLLTLPVKDWEVVVGKFLAGLALIALSLLLTFP
                     LVVTVKTLGNPDMGAIFASYLGSLLLGAAYLAIGFFVSSLTENQIVAFLLGITACFVF
                     FIIGENLVLISTPAPLVPFFRWLGLGTHFESIARGVLDTRDFLYYATIIGFFLFLNVR
                     SVAERKWA"
BASE COUNT         1172 a         2093 c         2241 g         1213 t
ORIGIN      
        1 ctcccccatg ccaccggcac cgaggggaga cacgatttcg taggggccga gtcgggcgcc
       61 gggggtgagt ggcatcggcg gatactacat gacgcggcac ccggcccggg gctgaagaac
      121 gcccgtcgtt acttcgccgg cttcccgggc gtccgcttct tcttcgcggc cgacgacttg
      181 aggatgagac tccccttccc tctggtcggg aacggagtct tcgcgtgccc gagcgcggcc
      241 atgatcagcg cctcgacgtc ggggttcttc aagcgactcg ccgattcgag ctggatgaac
      301 cgtgtcgtcc tggccgaccc ctggagaagc ttcttcgggt cgggcaactg cggcccctgg
      361 ctgaagtaga gaaacacgcc gtcggtggaa ccacgaagtg ccaggatccc atcgatcccg
      421 ttgttgctgg gcgaatagct gatgacgacg gcgtcaccgt agtcgtaggc cagctcgttg
      481 gcggccggga atcgcctgcg cagggccgtt cggaccgacc tgaagagcct ccggttcgtc
      541 ggatcgaggt tgtcgatgca ggccccaagc tgcgcctcgg atgtcggctc cccggtctcg
      601 cgtcttgcca tcacttcccc tcgcctcgca gtcgggcggt cagatcgaca gtcccacatc
      661 cccttcgaga accgacacgt tcaacttcgg acgctctccg ttcttccaga gtgccgcggt
      721 gtgcacgaca cgataccgga tcggcccgcc gtccgcgctc tcgctgcgcg tgatggggcg
      781 cgggatgcag acgaactcgg tgctcatctc ctcgctcgac aagcggaccc tggcgtagcc
      841 gtgcccgccg agatcgacga actcgaggtg cggcgccagg tccgggttgg aaagggcctt
      901 tgccttctcg agatccaggc tctccgcata ctcgaagcac gcgcggacac cgtgccggag
      961 cagcatgttg aatgtccagt ccggtttcgc gtcaccttcc ctgtccacga ggaacagcgc
     1021 tcgcagcggc gcgtccttcg gcagccggtg ctcgtaccct ccatcggccc gggactgacg
     1081 agagacccgc cgacgaagct caaaccaacc ggttcgaact tgcccggggg gagttccgcg
     1141 gcggcgtagc cggcccagaa actgtggcgg tctcccgaga cgatggcgaa accggtaatc
     1201 ccgttgtcgc gcaccagatc gcagatctcc ccgcgctccc gccacgcggt gccgtagtca
     1261 gcgggcccca tgttggcaaa tgtatcggga ggccaggcct cggtggtgag acctgcgggg
     1321 aggttattgg ggtcggcgcg atactcgagc gtgcccagcg agttgcccca gatcttccac
     1381 accgcggtgg acctcgtgag ctgatccttg aaccaggcct tctgctcggc accgagaatg
     1441 gtctgcggcg gcgcgcctcg ttgcggattg gccacatgcg cctcgttcca tcggagttcc
     1501 gaaggagggg tgccctcgcc aaaggcgcgc cccccgtcga ggacctgcat caatggttcg
     1561 ggaaacatgc ccatgaattc attgccgccc agctccccga gcgacggatc gctgaaggga
     1621 tccgcgctcc gataactgtg ctggtcggtg acgatcaggt ccagattccg accgtagcgc
     1681 agcgtccggt aggccctgag gctgttgatc gccgccaggt tgttcgcttc tgtccccagt
     1741 ccatcgctgt cgaactcttc gatggggacg tccttgaccg cgggcggatc gaaggtttcg
     1801 agcaactcgt cccgcccggc gatgcgtgcg ggaatgtatt cgaaccaggc ctggttcgcc
     1861 gccaccttga tgctctggcc cggttgctcg aacgaacccg ccttgacgat gctctgccac
     1921 ccctgccacg agaattcatg attgtcccag atcgccacga agggccagcg cgcacgggca
     1981 tcctggaggt ccggatcggt gagataggcc ttgtagatgg cgcgatagcc atcgacgttc
     2041 agcgggatgt gaaacttgcc gaccgtgtgg ccgtcctgga tgcgcaccac ctcatagatc
     2101 gtccggtcga ggcgcgtcgg cagctcgtcg gggtacatca ccacttcgta gatgaagtcg
     2161 ccgaggtgca ggacgaaatc gagcctttcc gcgtcggggg cgcgctcgtc ctcgtagatc
     2221 atccggcgaa aggcattgag cttcccctcg ttgatatcct gacagctcac gaaggcaaag
     2281 ttcaccgtgc gcggatcgtc cggcgcggga gccgtgatgg tgcggcctac ccgactccca
     2341 ttcccgtcgg catccgtgaa gcgataccag taggtggtgc ccggcttcag gccgccgacc
     2401 aggacccgcg aggtccaatc cgccgcgctc gaaaccggtg cctccgcatg cgcgatgacc
     2461 cggctgaacg cctcatcccc ggcgacctcg acggtcagga gctggcgcgt gccttcgtca
     2521 aacggacgtc tcgtccagag gatgacgctg tgcgcgtccg gatcgcccga ggccaccccc
     2581 tggggataga gatcccgccg ctcctgccac tggacccgcg aaacgcacgc cggtccggcc
     2641 cacgcgagcg acatgccgag tgctgctgac gttctgagga aggatcggcg gctgatggtc
     2701 atcgattcac tccgtccggc tgagggtggg ggttacctct ggacccagac gggaacgccc
     2761 gcaaggacgt tcagggccgt ccgttattta cattggcgtg acaacaatga ccgctgtcgg
     2821 cccgacgtca ggggatcaac ggcacctgcg atgcaaatct catctgaccg gccagcccca
     2881 ggcggcgatc cagctggatg gcacgggccg cgattcgcgg cgacaccacc tcctggaacc
     2941 tggccaggta cttcacgcga aggtcgagca gcttctgatc gcgcttcagg atcgaattca
     3001 cgtagtcgag cgcatcgtca tcggtcaact tcttgtagac atcgcggtac tgcaggaccg
     3061 acttcaactg ttcctcggtg atggccttct gttccttctg aaatgcctcg aacatcggcc
     3121 agaacctggc ggcctccccg ctggacagca cgagcccctc cgccagcaac tcggtgggcg
     3181 aagccgagat gtccgcccga aactgctcga tcacctggtc ctggttctgc caggtggaac
     3241 ccggggcggt caccgggtcc gccgcctggg cgggggtgat ggacatcgcg atcagcaaca
     3301 tgaggatgcg cttcagagtc atggctgtct ccagtacggc ggccaggccg cggggtggtg
     3361 gtgagggtca ggacgaagcc cgatcggcga gatcgtatcc catcgagcgc gcacgggacg
     3421 ccgtgggaaa gcctactgcg gcgtcggcgc tggcggttgc gccatttccc gcaattgatc
     3481 gtaccccttc ttcaaccgcg tgaaccgcca cggcccgatc atcaccaccg ccggatcccc
     3541 cacccgccgc acggcgtgct gtcccttcgc gagttttggg cccacttcga tcacggcgtg
     3601 gtcgccgttg gcggccgtca ggtcgaagcg gtagacgagc ggctcccagg caacagggat
     3661 cgtgtcctgc gcggtcggga acgtatcggc catgaggttg ccgagggtgt tggcgatgag
     3721 gcgcgcgtcg ttgggtttgg cgggcgatga ggcctcaccg gggagattgc ccgatacgct
     3781 ccaggtggag gtcttgaggc tgtcggttcc ttcccgcgtc agtgtcaccg tggtgtccgc
     3841 ccatgtcatc acgatggaac ggatctcctc ggccgggaat ggaacgacgc gcttgtcgcg
     3901 ccaggtctgc tggccccgct cgaagttgaa gcgggtgacc tggggaacgg aatagacttc
     3961 gttcttgccg tcaacgcgaa cgaaaaggcc gttgaagtcg ggggatgacg tgccgaccca
     4021 gaagttggcg ccaccgccac cttcgatgac gacgtggaca ccggaggtgt ccactccgaa
     4081 tgtcgattgc ttggcggggt tttcggaggc cacggagccg atgaccatcg tgtcgaccct
     4141 ggccagcatc tcctcgacgg ctttctggtc ggcgcggaag cgtccctcgc tcacgatcca
     4201 ggtgccgccg tctttttcga ggttggtgat gttgccggcg gtggtgatgg ccgcgtgcgt
     4261 ggcgcctgtc gggtcgaagt ccttgaacac gcggcctttt ctcgtggaag ggccgcgaat
     4321 gtcgtccttg taagggcgct ccacgagcag gacccacgcg ccgaggagaa tgagaatgcc
     4381 gaagaggatg ttgcgagttc tcggattcat ctagcggtac cgggcggcca tgttctgggc
     4441 gcggcggcgg ttgtaccagc ggaagaggcc ccaggcgccg acgaggagcg gcatcaccag
     4501 catgttggcg agcttgatgg ggccgcgggc gttgtcgggc acgggcttga gcggccggct
     4561 gaagttggcg cgggagcgga tggcgatgag ctggtcgccc agcgtgcacc aatccacggc
     4621 gttcaggaaa aaggtgaggt tctcggggta ctggctcgtg aactggtcac tggcgaagtc
     4681 ggcatcggcc acgacgatga tctgcgtgga cgggctccgg tcgaccgtgg ccggatttgg
     4741 cgccatggcg gctgccgcgc tgtcgggagg gggcggagcc gggcgaccgg cgaaggctga
     4801 cgcgaaggtt cccgtcaacg acaccgccac cgggtagctc gtcatttccg agggattcgg
     4861 cggagccagt cgctgctggg gattgatgct cgtctgaccc tcgaggcgga acgactgctt
     4921 cgtggtcttc gccaggattt cggcatggac gccaccctcc ggcgcggcgc cggtagtgtc
     4981 gaccggcacg gtcgtctcga gcgagctcac ccacggggtc acgatcgact gcaacgtgga
     5041 agtgaccggg tgcttcctgt tcaggttgtc gggcaggatc cgcgggaaga aggggtacgg
     5101 catcatgaac gagaatgagc cgttcgagaa cgcggtggag acgtgcgagg cgtcgagcaa
     5161 caggtcgctg ccgatgcgcg cgccgtaatg cgaaaggagt tcgttgagcc ccgaatcgaa
     5221 aggctgggcc atgagttgct gcggaacgat ctgcgtaccg tcggccagga cgatgagccg
     5281 ccccccgcgc atcacgaact ggtcgatctc gtagcggtcc cgatcgccaa gacgttgcgt
     5341 ggggcgggcg acgatcagcg tgcggatgtc ggccggcacc ggctggccgc cggagatatc
     5401 gacattggtc acctcgtact gcttctggag cgcctggccg gcgcccgaca tctggccgcg
     5461 ctccagggtg gcctcgccgt ggccggtcag gagacccacc ttcggcggcg cctgtgtcac
     5521 tttcaatatc gcgctggtca ggtcgtactc gaggttctcg gtggactgca cgatcgggat
     5581 cgtctcgctc ttgtccgcgt actggatggc gatgccgagg tacacgttcg tgacctgggc
     5641 cttttccttc tcgaggatgt tgagctgcac cttgggaatc ccgatcatct gcagcgatcg
     5701 ctccttcgtc gggtcgcttc ccgggtcctc gaactggatg cgcagcttgc cgcccgagaa
     5761 agccttgtac tcgtcgagga ggtccttgac ctggcgatcg agcgtggcaa gatacggcgg
     5821 caagtccttc gaaaagtaga cggtgacgtt caccacgtcg tcgagtccgg ccaggatggc
     5881 tttcgtcgac ttcgtgaccg tgtactccct gttctgggtc aggtcgaggc gggcgaaccg
     5941 tttcaccgcg atgatgttga cgagtcccag gatggccacg atgaggagca ccacgccgat
     6001 cgagctcccc gatccgaaga gtcccttgcg cgcgggcatc aggcccactt cctttcggcc
     6061 accgagcgga cgttcagaaa aaggaaaaat ccgatgatcg tggcgtagta gaggaagtcc
     6121 cgcgtatcga gcacgccacg ggcgatactc tcgaagtgcg tgccaaggcc aagccagcgg
     6181 aaaaagggca ccagaggcgc cggcgtcgaa atcagcacca ggttttcgcc gatgatgaag
     6241 aagacgaagc aggccgtgat gccgagcaga aaggccacga tctggttctc ggtgaggctc
     6301 gacacgaaga agccgatggc caggtaggcc gcgcccagga ggagcgagcc gaggtaactc
     6361 gcgaaaatgg cccccatgtc cgggttgccg agcgtcttga ccgtgacgac cagcgggaag
     6421 gtgaggagca gcgacagcgc gatgagcgcg aggcccgcca ggaacttgcc caccaccact
     6481 tcccagtcct tgaccggcag ggtgaggagg agctcgaccg tgcccatctt cctttcttcc
     6541 gaccagaggc gcatggccac ggccggcacg aagaaaagga agtagagggg catcgtgccg
     6601 aagaggctgc ggagcgaggc ctgctcggcc aggaagaaat cgcggaagaa gaaccagttg
     6661 accacgacca ggaagaccgc gatgtagacg tacgcgatcg gcgagtcgaa gtacgactt
//
LOCUS       DASVOD010000731         4073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_412118, whole genome shotgun sequence.
ACCESSION   DASVOD010000731 DASVOD010000000
VERSION     DASVOD010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412118"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1354
                     /locus_tag="VF720_15760"
     CDS             86..1354
                     /locus_tag="VF720_15760"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX7880868.1"
                     /translation="MNRDVYALLLAGGVGSRLSILSAKRAKPAVPFGGKYRIIDFTLS
                     NCVNSGVDDIGILTQYRPMSLNEHIGNGKAWDLDRMTGGVTLLQPFLGRQTGDWYVGT
                     ADAVYQNLDQVTRRKVEHVLILSGDHVYNMDYNRMIRFHEEKGADVTIAVTTVPWEMT
                     DQFGIVETDENARVVGFEEKPARARSNLVSMGVYVWSARALVRRLTEDAAKPDSQHDF
                     GRNIIPAMIPDGKAFGYRFDGYWQDIGTLEAYYEANLNLVSDRPQLDLFDPEWKIHTP
                     SAERPPVKFGSGGRSQYSLIANGCVIQGNVERSVLFPGVFVGPGAHVSDSIIMNDTRI
                     EAGARIDRTILDKQITVGERAVIGHGDASVANIRIPLLLHSGLTVVGKSTVIPKDVKI
                     GRNCCLAADLTEADFTSLELPPGSVLGDTG"
     gene            1582..2448
                     /locus_tag="VF720_15765"
     CDS             1582..2448
                     /locus_tag="VF720_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX7880869.1"
                     /translation="MPYVTTRDKTRLYVKDWGSGPPVVLLHGWPLNADTWDGVALELA
                     EAGFRAIAYDRRGFGRSDQPWNGYDYDTLADDLAAVMAETGAIDATLVGFSMGGGEVA
                     RYMSRHEGKGVARSVLIGSVVPHMTKSADNPEGVEGETFAKMAAAIREDRAHFFRGFF
                     KDFYGVGMIARPVSAEVLDWSLAMALQAGLRPTLAAADAFARTDFRADLASFQVPTLF
                     LHGTADKTVPIDATSRVAAARVTGAEFIEYDGGPHGLLATHPGEIATDLLAFLGRPVE
                     VSSGMVPGTTVI"
     gene            complement(2477..>4073)
                     /locus_tag="VF720_15770"
     CDS             complement(2477..>4073)
                     /locus_tag="VF720_15770"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX7880870.1"
                     /translation="TPAAGPTEPAPDGAPLAQLPPPETLDPDTESVPDSTDTARILIH
                     VPRIETPFDDAHPQDSQVIHAWERVYYANLDMEPSEPDSVLFFRYQALLTHLDPGMIS
                     AEAPRLRDRAVDLRQWITRSRDAAWQRWQVDLQPPPEPAPVDDTNLAAITPDDHPRVQ
                     KWINVFTGPSRERFAIWIYRSGAYRPLMEQILKEEGVPVELIAMVFIESGFSLVAKSR
                     ASAVGPWQFIRETGKRYGLTINNHRDERRDFVLATRAAARYLRDLYGYFGDWKLAMAA
                     YNCGEGRVFRQISKQGTNDFYALDLPRETEDYVPEIHAALAIIRDPQAYGFSTVIADP
                     IEYVEQELPGPVRVADLAAHCAMSIDEVKLLNPAWLRAVTPADGKPVTARLPGSIMPG
                     ISLASLPLAPKEETVARNATVKVRKGDTMSKIARRNGVSVSALARENGISTKAKLKAG
                     RYLRLPDGVGGGSSSSGKSSGSSKKSKSSSSGNGVAFHTVKRGDTLSAICERYGLSMN
                     ELKRLNGLSGSSIQAGQKLKLRG"
BASE COUNT          658 a         1292 c         1433 g          690 t
ORIGIN      
        1 gcgtcatcga accgggcgtc cacgcggtgg acttcgacgg cacgccggtc gagtccggct
       61 cgcgcgtccg ccgcgggggt gacgcgtgaa ccgcgacgtc tacgccctcc tcctggccgg
      121 aggcgtcggc agccgcctct cgatcctgtc ggccaagcgg gccaagcccg cggtgccgtt
      181 cggcggcaag taccggatca tcgacttcac gctttccaac tgcgtgaact cgggcgtgga
      241 tgacatcggc atcctcaccc agtaccgccc catgtcgctc aacgagcaca tcggcaacgg
      301 caaggcctgg gacctcgacc ggatgaccgg aggcgtcacg ctgctgcagc ccttccttgg
      361 ccgccagacc ggcgactggt acgtggggac cgccgacgcg gtgtaccaga acctcgacca
      421 ggtgacccgc cgcaaggtgg agcatgtcct catcctctcc ggcgaccatg tctacaacat
      481 ggattacaac cggatgatcc gcttccatga ggagaagggg gccgacgtca ccatcgccgt
      541 cacgaccgtg ccgtgggaga tgaccgacca gttcggcatc gtggaaaccg atgagaacgc
      601 tcgcgtcgtg ggcttcgagg aaaagccggc gcgcgcgcgc tcgaacctcg tctccatggg
      661 cgtctacgtc tggagcgcca gggcgctcgt gcggcgcctc accgaggatg cggcgaagcc
      721 cgacagccag cacgacttcg gccgcaacat catcccggcc atgattcccg acggaaaggc
      781 cttcggctac cgcttcgacg gctactggca ggacatcggc actctggagg cctactacga
      841 ggccaacctg aacctcgtga gcgaccgccc gcagctcgac ctcttcgacc ccgagtggaa
      901 gatccacacg cccagcgcgg agcgcccgcc cgtgaaattc ggttccggcg gacgttcgca
      961 gtacagcctc atcgccaacg gctgcgtcat ccagggaaac gtcgagcgct ctgtcctctt
     1021 cccgggcgtc ttcgtcggcc cgggcgccca cgtgtcggac tccatcatca tgaacgacac
     1081 tcgaatcgaa gccggcgccc gcatcgaccg caccatcctc gacaagcaga tcaccgtggg
     1141 cgagcgcgcc gtcatcggcc acggcgacgc ctcggtggcc aacatccgca tcccgctgct
     1201 gctccactcc ggcctcaccg tcgtgggaaa gagcaccgtg atcccgaagg acgtgaagat
     1261 cggccggaat tgctgcctgg ccgctgacct gaccgaggcc gacttcacgt cgctggagtt
     1321 gccaccgggg tcggtgctgg gggatacggg gtagcaggcg acgccgcgac tgccgatgtc
     1381 gaaggacctg tgatggaaag agtattccag ttgtcctgat tcgcgcggcg aagccgcgct
     1441 gcccgcatcc cacacctctc ctgcgtgatt agggcttccc cggatagtca gcttcccccg
     1501 gaaccacgac catgacattc gtgaaggatc aaccgttgat cctggcctgg ccgctttgga
     1561 ataccttgag aggagatggc catgccttac gtcaccaccc gcgacaagac gcgcctgtac
     1621 gtgaaggact gggggagtgg tccgccggtc gtgctgctgc acggatggcc gctgaacgcg
     1681 gatacctggg acggggtggc gctcgagttg gcggaggcgg ggtttcgggc catcgcgtat
     1741 gaccggcggg gattcgggcg gagcgaccag ccgtggaacg gttacgacta cgacacgctg
     1801 gcggatgatc tggcggcggt catggcggaa acgggcgcca ttgacgccac gctcgtcggg
     1861 ttctcgatgg gtggcgggga ggtggcgcgc tacatgtcgc ggcatgaagg gaaaggcgtg
     1921 gcgcggtcgg tcctgatcgg gtcggttgtc cctcacatga cgaagtcggc cgataatccg
     1981 gaaggggtgg aaggggagac gttcgcaaag atggcggcgg cgatccggga agaccgggcg
     2041 cacttcttcc gcggcttctt caaggacttc tacggcgtgg ggatgatcgc gcgtccggtg
     2101 agcgccgagg tgctcgactg gtcgttggcg atggcgctcc aggcgggact gcgacccacg
     2161 ctggcggccg ccgatgcctt cgcgcggacc gacttccggg ccgacctggc ctcgttccag
     2221 gtgccgacgc tcttcctcca cggcacggcc gacaagacgg tgccgatcga tgccacctcg
     2281 cgggtggctg cggcgcgggt gacgggcgcc gaattcatcg aatacgacgg cggcccgcat
     2341 ggactcctgg ccacgcaccc gggggagatt gccaccgacc tcctggcctt cctgggaagg
     2401 ccggtggagg tatcgtcggg gatggtgccc ggtacgacgg tgatctgagc ggcgggcggc
     2461 ggagcggccg gctcgatcag ccccgcagct tgagtttctg tccggcctgg atggaggagc
     2521 cggagaggcc gttcaggcgc ttcagctcgt tcatgctgag gccgtagcgc tcgcagatgg
     2581 ccgagagggt gtcgccgcgc ttcacggtgt ggaaggccac gccgtttccc gaagatgacg
     2641 acttggactt cttcgaggag ccggatgact ttccgctgct gctgctaccg cccccgacgc
     2701 cgtcgggaag gcggaggtag cgaccggcct tcagcttggc cttcgtgctg atgccgttct
     2761 cgcgggccag cgcgctcacc gatacgccgt tgcgcctggc gatcttggac atcgtgtccc
     2821 ccttgcgcac cttcacggtg gcgttgcggg ccacggtctc ttccttgggc gccagcggca
     2881 acgaggccag cgagataccg ggcatgatgg agccggggag acgggcggta acgggcttgc
     2941 cgtcggccgg cgtcacggcg cggagccagg cggggttcag gagcttcacc tcgtcgatcg
     3001 acatggcgca gtgggccgcg agatccgcca cgcggaccgg tcccgggagt tcctgttcca
     3061 cgtactcgat cgggtcggcg atcacggtcg aaaagccata ggcctgagga tcccggatga
     3121 tcgcgagcgc ggcgtggatc tccggcacgt aatcttcggt ttcccgaggg aggtcgagtg
     3181 cgtagaagtc gttggttccc tgcttcgaga tctggcggaa gacacgcccc tcgccacagt
     3241 tgtaggcggc catggccagc ttccagtcgc cgaagtagcc gtagaggtcg cgcaggtagc
     3301 gggcggcggc gcgggtggcc aggacgaaat cgcggcgctc gtcgcgatga ttattgatag
     3361 tgaggccgta ccgctttccg gtttcgcgga tgaactgcca tggccccacg gcgctggccc
     3421 ggctcttggc caccaggctg aagccgctct cgatgaagac catcgcgatc agctcgaccg
     3481 gcaccccctc ctccttcagg atctgctcca tcagggggcg atacgcgccg gagcggtaga
     3541 tccagatggc gaaccgttcg cggctgggcc cggtgaaaac gttgatccac ttctgtaccc
     3601 gcggatggtc gtcgggcgtg atggccgcaa ggttcgtgtc gtcgacgggg gccggctcgg
     3661 ggggcggctg aaggtccacc tgccagcgct gccaggcggc gtcgcggctc ctggtgatcc
     3721 attgccgcag gtcgaccgcc cgatcgcgga gacgcggcgc ctcagccgag atcatgcccg
     3781 gatcgaggtg agtgagcagg gcctggtaac ggaagaagag gaccgagtcg ggctcgctcg
     3841 gctccatgtc gaggttggcg taatagaccc gctcccaggc atgaatgacc tggctgtcct
     3901 ggggatgggc gtcgtcgaaa ggggtctcga tccgcggcac atggatcagg atgcgtgccg
     3961 tgtccgtgga gtcggggacg ctttcggtgt cggggtcgag ggtttcggga ggcgggagtt
     4021 gggcgagggg ggcgccgtca ggggcgggtt cggtggggcc ggctgcgggg gtc
//
LOCUS       DASVOD010000732         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_466887, whole genome shotgun sequence.
ACCESSION   DASVOD010000732 DASVOD010000000
VERSION     DASVOD010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466887"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF720_15775"
     CDS             complement(<1..334)
                     /locus_tag="VF720_15775"
                     /inference="COORDINATES: protein motif:HMM:NF014666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX7880871.1"
                     /translation="MLIRAGACYSPGMRDTRSLDGIFRPQSVAVVGASNRPGNIGREI
                     VHNLIEFEFQGPVFPVNPHLRTLHSLKAYPTIEAIPDPVDLAVIVVPKEQVNRVVSAC
                     GEKGVKGLV"
     gene            379..1179
                     /locus_tag="VF720_15780"
     CDS             379..1179
                     /locus_tag="VF720_15780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX7880872.1"
                     /translation="MNEELTIVSRIAREAGDLLLSRFGTGIQIELKGEVDLVTEMDRR
                     SEELIVRRLAEEFPGTAVLAEEGERHADTGAGLWIVDPLDGTTNYSHGFPVFAVSIAF
                     ERNGVVELGVVHDPTRNECFVASRGGGAHLDGRPLAVTTQSELGSSLLATGFPYDIRT
                     SPRNNLAEFGRFALRARAVRRAGAAALDLAYVAAGRFDGFWEEKLAPWDVAAGALMVV
                     EAGGVVTGFRGEPPVIRTGRLVASNGRIHAAMVATLAETASGTDASGR"
     gene            1094..>2311
                     /locus_tag="VF720_15785"
     CDS             1094..>2311
                     /locus_tag="VF720_15785"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7880873.1"
                     /translation="MWPATGGSTRRWWPRWPKRLQERTLPADNSRDGEEPFAAPAATL
                     EARLNLTILIVEENPAARESWVEGLRSAGHRVFSFAAARSALDSLGATPVDAAFLSFK
                     DPELCRELQAMLKQRNAACQVTLMTDRPAEDKDAQALESGAREVLLRPFAPERLGSLV
                     ERMSSSREKRTGRAITGGAATLLGESEPMAAVRRLIGQVAASPDTSVLVTGESGTGKE
                     LVARAVHAESQRSQQPFLEINCAAIPETLLESELFGHEAGAFTDATRARAGLFELADR
                     GTLFLDEIGEMGLDLQAKLLRVLDTRSVRRVAGSELKKLDLRILAATNRDLDAEVKDR
                     RFRGDLFHRLNVVHIHLPPLRERRSDVDLLARHFIGVFGRKFGRAVHQPDDLLLERLR
                     RYDWPGNVRELANS"
BASE COUNT          372 a          814 c          754 g          371 t
ORIGIN      
        1 ccacgaggcc tttcacgccc ttttcgccgc aggcgctcac gacgcggttc acctgttcct
       61 tcggcaccac gatcaccgca aggtccaccg ggtccgggat cgcctcgatg gtgggatacg
      121 ccttcaggct gtgcagcgtc cggagatgcg ggttgaccgg gaagacgggg ccctggaatt
      181 cgaattcgat caggttgtgg acgatttcgc gcccgatgtt tcccggacgg ttggaggcgc
      241 cgacgacggc cacggactgc ggccggaaga tgccgtcgag ggaacgggtg tcgcgcatgc
      301 cgggactgta gcaggcgccc gcccggatga gcatgccgcg cgaggcactg ttaagatgcc
      361 ccgatgtcca cgactcccat gaatgaggaa ctgacaattg tctcccgcat tgcacgcgag
      421 gccggcgacc tcctcctctc gcgtttcggc acgggcatcc agatcgagct caagggggag
      481 gtcgacctgg tcacggagat ggaccgccgt tcggaagagc tcatcgtccg ccggcttgcc
      541 gaggaattcc cgggcacggc ggtgctggcc gaggaagggg agcgccatgc cgacacgggc
      601 gccggtctct ggatcgtcga tccgctcgac ggcaccacca actactcgca cggatttccg
      661 gttttcgcgg tgagcatcgc gttcgagcgc aacggcgtcg ttgagctcgg cgtcgtgcac
      721 gatcccaccc ggaacgaatg tttcgtcgcg tcacggggcg gcggcgcgca cctcgacggc
      781 cggcctctcg cggtcaccac gcagtccgaa ctcggcagct cgctcctggc caccggtttt
      841 ccatacgaca tccgcacgtc gccccggaac aaccttgccg aattcggccg ctttgccctg
      901 cgggcgcggg ccgttcgccg ggccggcgcc gccgcactcg acctggccta cgtggccgcc
      961 ggccggttcg acggcttctg ggaggaaaag ctcgccccct gggacgtggc cgccggggcc
     1021 ctcatggtgg tcgaggccgg gggagtggtg accggattcc ggggtgaacc ccccgtcatc
     1081 cggacgggcc gccttgtggc cagcaacggg cggatccacg cggcgatggt ggccacgctg
     1141 gccgaaacgg cttcaggaac ggacgcttcc ggccgataac tcccgggatg gggaggagcc
     1201 gtttgcggct ccggccgcaa ccctggaggc acgactgaac ctgaccatcc tgatcgtcga
     1261 agagaatccg gctgcccgcg aatcgtgggt ggaggggctc cgctcggcgg ggcaccgcgt
     1321 cttctcgttt gccgccgccc gctcggctct ggacagcctg ggcgcgactc ccgtcgatgc
     1381 cgccttcctc tcgttcaagg accccgagct ctgccgcgaa ctccaggcga tgctcaagca
     1441 gcgcaatgcc gcctgtcagg tcactctcat gaccgaccgc ccggccgagg acaaggacgc
     1501 acaggccctc gaatcaggcg cccgcgaagt gctcctccgg ccattcgcgc ccgaacgtct
     1561 cggctccctc gtggaacgga tgagctccag ccgcgagaaa aggaccggcc gggccatcac
     1621 cggcggcgcc gccacactgc tcggtgaatc cgaaccaatg gccgccgtcc gacgcctgat
     1681 cggccaggtg gccgcctctc cggacacgtc cgtgctcgtg actggcgaaa gcgggaccgg
     1741 caaggaactc gtggcccgcg ccgtccacgc cgagagccag agatcgcagc agcccttcct
     1801 cgagatcaac tgcgccgcca tccccgaaac cctgctcgaa agcgagctct tcggccacga
     1861 ggccggcgcc ttcacggacg ccacgcgggc ccgggcgggc cttttcgagc tggccgatcg
     1921 cggcacactc ttcctggacg aaatcgggga gatgggactc gatcttcagg ccaagcttct
     1981 ccgggtgctg gacacccgtt ccgttcgacg cgtggccggg agcgaactca agaagctcga
     2041 cctccgcatc ctggcagcca ccaatcgcga tctcgatgcc gaggtgaagg atcgccgctt
     2101 ccgcggcgac ctcttccatc gactgaacgt ggtccacatc catctgccgc cgctccgcga
     2161 gcgtcgctca gatgtcgatc tccttgcccg acacttcatc ggggtattcg gccgcaagtt
     2221 cggccgggcg gtccaccagc cggatgacct gctcctggaa cgcctgcgcc gctacgactg
     2281 gccgggcaac gtgcgcgaac tggccaacag c
//
LOCUS       DASVOD010000733         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_309789, whole genome shotgun sequence.
ACCESSION   DASVOD010000733 DASVOD010000000
VERSION     DASVOD010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309789"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VF720_15790"
     CDS             complement(<1..917)
                     /locus_tag="VF720_15790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7880874.1"
                     /translation="MTALDKFLRSARAPAWAFFCLALALRAGVVLFRFGDELGFADER
                     MYLRLADVIGNGQWLGEGTAAAPGPVYFWGALRAMGMSLLGIRLVHALAGAAAVAVLF
                     LIARRLVGPIPAALAALVMSVYPYLLFLSGLLYPQAVFHLTFLVMIYGLLRWMQDGGT
                     RWFVLGGVALGVSALFVVPILSASPLVALWVVLAGRGPFFARVRDAVVLGLITLATIL
                     PATARNYLVSRQLILLSSMGGSAFYWANNDQADPYNRDADVWLEKYQRRQDREQARQG
                     WDAPDMQELSSYRASRFWREQTPRALRNY"
     gene            943..>2327
                     /locus_tag="VF720_15795"
     CDS             943..>2327
                     /locus_tag="VF720_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX7880875.1"
                     /translation="MRILAPLLAVLVAALVIAGAFDIHRRPYKGFAVRDDRVAVVDAG
                     SPADRAGLSVGDRIQRVFVNSPGDTGGVTLTESRVADLGTRPRGTMAGLLVTPPSGSP
                     ARVVSFELTAPPAAESWRLSLSVLVALICLGTGWLTWHLRPGGMSRAFLVICVALATI
                     LRPPLPPNPAWLASLELLLSDLAAMALAAALVEFVMRLPAGRLPRDSRPFVMAAWLMA
                     LFLGAAEVALQLGLPFPEFAMPLASGLAIVFVVGCLLGALARFFVTLARTSDRADALR
                     LRVVMWGGLLGLSPITLILILRNLTPLPVVAGEQFAVLAVAFLPLAWMYAIVRHRIFD
                     IRLLMRRGAVAFIFTTILAAVWLGAVAWLGTRLDPVTAHPLGAVAGLLVAALLFTGSR
                     HYVQAFVDRTFFSADLSRRRRLEELSGRLSGFLDSERLTESLVAELDHRLGAIRSTLL
                     HPSANGRGDGPA"
BASE COUNT          332 a          828 c          788 g          379 t
ORIGIN      
        1 atgtagttcc ggagggcccg gggcgtctgc tcgcgccaga agcggctggc gcgatagctc
       61 gagagctcct gcatgtcggg cgcatcccaa ccctggcgag cctgctcgcg atcctgccgc
      121 cgctggtatt tctcgagcca gacgtcggcg tcgcggttgt agggatcggc ctggtcgttg
      181 ttggcccagt agaaggccga gccccccatc gaggacaaca ggatgagctg ccggctcacc
      241 aggtagttgc gcgccgtggc cgggaggatc gtggccagcg tgatcaggcc gagcaccacg
      301 gcatcgcgca cgcgcgcgaa gaacgggccg cggccggcca gcaccaccca gagggccacc
      361 agcggcgagg ccgaaaggat cggcaccacg aagagcgccg aaacacccag cgccacgccc
      421 ccgaggacaa accagcgcgt cccgccgtcc tgcatccagc ggagcagccc atagatcatc
      481 acgaggaacg tgagatggaa caccgcctgc gggtagagga ggccggagag aaagagaagg
      541 tagggataca ccgacatcac cagggccgcc agagccgccg gaatgggacc caccagccga
      601 cgggcgatga ggaacaggac ggcgacggcg gcggcgccgg ccagcgcatg gaccagccgg
      661 atcccgagga gggacatccc catggcgcgg agggctcccc agaagtagac cggccccggg
      721 gccgccgccg tcccctcgcc gagccactgc ccgtttccga tgacatcggc cagccggagg
      781 tacatccgct cgtcggcaaa gcccagttcg tccccgaagc gaaaaaggac gacgccggcc
      841 cgcagggcca gcgcaaggca gaaaaaggcc caggcggggg cccgggccga acgtaggaac
      901 ttgtcgagtg cggtcacggc ggtggattat acccgagacg tgatgcgcat cctcgccccg
      961 ctcctggccg tccttgtggc ggcactcgtc atcgccggcg ctttcgatat ccatcgacgc
     1021 ccatacaagg gctttgccgt tcgcgatgac cgggtggccg ttgtcgacgc cggcagtccc
     1081 gccgatcgcg ccggcctttc ggtcggcgac cggatccaac gcgtcttcgt gaactctccg
     1141 ggcgatacgg gaggggtcac gctcaccgaa agccgtgtcg ccgatctcgg cacgcggccg
     1201 cgtggcacga tggccgggct cctcgtcacg cctccctcgg gttcgcccgc ccgcgtcgtt
     1261 tcgttcgaac tcaccgcgcc gccggccgcc gaatcgtggc gactgtcgct ctccgtcctg
     1321 gtggcgctga tctgcctcgg cacgggctgg cttacctggc acctgcggcc gggcgggatg
     1381 agccgcgcgt tcctcgtcat ctgcgtggcg ctggccacca tccttcgacc cccgttgccg
     1441 ccgaacccgg catggctggc ctcgctcgag ctcctcctct ccgaccttgc cgccatggcg
     1501 cttgcggccg cgctggtgga gttcgtgatg aggttgccgg ccggacgact cccccgcgat
     1561 tcgcgaccgt tcgtgatggc cgcgtggctg atggcactct tcctcggggc ggccgaggtg
     1621 gcactgcaac tgggtttgcc gtttcccgag ttcgcgatgc cgctcgcctc ggggctggcc
     1681 atcgtcttcg tcgtggggtg cctgctcggg gcattggccc gctttttcgt cactctggcc
     1741 aggacgagcg atcgggccga tgcgctgcga ctccgggtgg tgatgtgggg cggtctgctc
     1801 gggctctcgc cgatcacgct catcctcatc ctgcgcaacc tcacgccgct ccccgtggtg
     1861 gccggcgagc agttcgcggt gctggcggtc gccttcctcc cgctggcctg gatgtacgcc
     1921 atcgtccgac atcgcatctt cgacatccgg ctcctcatgc ggcgcggcgc ggtggccttc
     1981 atcttcacca ccatcctcgc ggcggtctgg ctcggcgcgg tggcctggct cggcacgcga
     2041 ctcgatccgg tcacggctca cccgctgggc gcggtggccg gactgctcgt ggccgcgctc
     2101 ctgttcaccg ggtcgcggca ctacgtgcag gccttcgtcg atcgcacgtt cttcagcgcc
     2161 gatctctcgc gccggcggcg cctcgaagag ttgtcggggc ggctcagcgg cttcctcgat
     2221 tccgagcgcc tcaccgaatc actggtggcc gagctcgatc atcgcctcgg tgccatccgt
     2281 tccacgctgt tgcacccgtc ggcgaacggc cggggcgacg ggccggc
//
LOCUS       DASVOD010000734         2471 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_569869, whole genome shotgun sequence.
ACCESSION   DASVOD010000734 DASVOD010000000
VERSION     DASVOD010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569869"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..609
                     /locus_tag="VF720_15800"
     CDS             <1..609
                     /locus_tag="VF720_15800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880876.1"
                     /translation="IDDIQGVVEKIDGAIQRLAALKHGEEIALAPTDLEWILDSVLTR
                     RGVSMRPGYSVARNFGGVPSVVADQLLIDRVLTNLVTNAMEAMPEGGVLTLETEDVRE
                     AGSGGRWAVVRVRDTGTGMTQAFLRHRLFQPFATTKKKGWGLGLYQCRSIVEAHGGSL
                     RANSQPGLGTEFRLALPLTLTAARREDRGLPHPADRHGRRPQ"
     gene            619..2016
                     /gene="prsR"
                     /locus_tag="VF720_15805"
     CDS             619..2016
                     /gene="prsR"
                     /locus_tag="VF720_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEX7880877.1"
                     /translation="MHTVETAVRPRLLMVDDDAALLRQLTWGLKEHFEVTTASTVENA
                     LERVRSDKPGIITLDLALSEGGQTDEGFMLLDEMLAIDRSLKIILITGNDTRENALKA
                     IDRGAFDFFSKPIDLDELRILLRRAAQLRALEEENARLQEHLRASGGMGGIHGSSEEI
                     QRVFQMIRRVATTDVTVLITGESGTGKELVAREIHRQSHRADRPFISIACGAIPEQLL
                     ETELFGHEKGSFTGAHTAREGKLEMANGGTVFLDEIGELPLSLQVKLLRFLQEKEIER
                     VGGRRIIPLDVRILTATHRDLRKEIERGGFREDLFFRLSVVNVALPPLRDRRDDVLFL
                     ARHFLNRFGQEFRRGPLHFSREALRVMQRYEWPGNVRELEHRVQRAVVMSTGRTVRPT
                     DLDLAVASSLSVAPLKTVRENAERQLLVDSLKRNCGNITRSARELAISRPTYHDLLKK
                     YSIRAGEYKNGQGPE"
     gene            2013..>2471
                     /locus_tag="VF720_15810"
     CDS             2013..>2471
                     /locus_tag="VF720_15810"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7880878.1"
                     /translation="MSTTNGNGTPWSPHLIETLVMDPWTIHAVADARFQADGGVAFAM
                     VPRALWEREATVVEGNSIPLRVGVLLMERPGVRLLIDTGVGDDGVPRNVSGFFGGLSA
                     TGGLDRALNLLGWERDSITHVLLTHLHIDHAGGLFAQDETPRFPGAKVIVS"
BASE COUNT          430 a          904 c          773 g          364 t
ORIGIN      
        1 atcgacgaca tccagggggt ggtcgagaag atcgacggcg ccatccagcg cctcgcggcc
       61 ctgaagcacg gcgaggagat cgcgctggcc ccgaccgacc tcgaatggat cctcgacagc
      121 gtgctcaccc ggagaggcgt ctcgatgcgt cccggctaca gcgtggcccg caacttcggg
      181 ggcgtaccct cggtggtggc cgaccagctc ctcatcgacc gcgtgctgac caacctcgtc
      241 accaatgcca tggaggccat gcccgagggg ggtgtcctca cgctcgagac ggaggacgtg
      301 cgcgaagccg gttccggcgg ccgctgggcc gtggtccgcg tgcgcgatac cggcaccggg
      361 atgacgcagg ccttcctccg tcaccgcctc ttccagccgt tcgccaccac caagaagaaa
      421 ggttggggcc tcggcctcta ccagtgtcgc tccatcgtcg aggcccacgg cggttcgctc
      481 cgcgcgaaca gccaaccggg actcggcact gaattccgac tcgccctgcc gctgacgctc
      541 accgccgcac gccgcgaaga ccggggattg ccgcacccgg ccgaccgcca cggacgccga
      601 ccgcaatgag gaccttgaat gcacaccgtt gaaactgccg tccgaccgcg cctcctgatg
      661 gtcgacgacg acgcggcact gctccgacag ctcacgtggg gattgaagga acacttcgag
      721 gtgacgacgg ccagcaccgt cgagaacgcc ctggagcggg tacggagcga caagcccggg
      781 atcatcactc ttgacctggc cctttccgag ggtgggcaga ccgacgaggg attcatgctc
      841 ctcgacgaga tgctggccat cgaccgctcg ctcaagatca tcctgatcac cggcaacgac
      901 acgcgtgaaa acgcgctcaa ggccatcgac cgcggtgcct tcgacttctt ctcgaagccg
      961 atcgacctgg acgaactccg gatcctgctc cgtcgcgccg cccagctccg ggccctcgag
     1021 gaagagaatg cccggctcca ggagcacctg cgcgcctccg gcggaatggg ggggatccac
     1081 ggctcgagcg aggagatcca gcgcgtattc cagatgatcc gccgggtggc caccaccgac
     1141 gtcacggtgc tcatcaccgg cgagagcggc accggcaagg aactggtggc ccgcgaaatc
     1201 caccgccaga gccaccgcgc cgaccggccg ttcatctcca tcgcctgcgg cgccatcccg
     1261 gaacaactcc tcgagaccga gctgttcggt cacgagaaag ggtcattcac cggcgcccat
     1321 accgcccgcg agggaaagct ggagatggcc aacggcggaa cggtcttcct ggatgagatc
     1381 ggcgagctcc ccctttcgct ccaggtgaag ctcctccgct tcctccagga aaaggagatc
     1441 gagcgggtcg gggggcggcg catcatcccg ctggatgtgc gcatcctgac cgccacgcac
     1501 cgcgatctcc gcaaggagat cgaacggggc ggattccgcg aagacctctt cttccgcctg
     1561 tcggtggtga acgtggcgct gccgccactc cgcgaccgtc gcgacgacgt gctcttcctg
     1621 gcccggcact tcctcaatcg cttcggccag gaattccggc gcgggccgct ccacttcagc
     1681 cgggaggccc tccgcgtgat gcagcgctac gaatggcccg gcaacgtgcg cgaactcgag
     1741 caccgcgtgc agcgcgcggt ggtcatgtcc accggccgca cggtgaggcc caccgacctc
     1801 gacctggcgg tggcctccag cctctcggtg gctccgctca agacggtgcg cgagaacgcc
     1861 gagcgccagc tcctcgtgga ttcgctcaag cgcaactgcg gcaacatcac ccgttcggcc
     1921 cgcgaactgg ccatcagccg ccccacgtac cacgacctgc tcaagaaata ttcgatccgc
     1981 gccggcgagt acaagaacgg ccaggggcct gagtgagcac gaccaacggt aacggaacgc
     2041 cctggtcgcc ccacctcatc gaaacactgg tcatggaccc gtggacgatc cacgccgtgg
     2101 ccgacgcccg tttccaggcc gacggcggcg tggccttcgc catggtgccg cgcgccctct
     2161 gggaacgtga ggccaccgtg gtggagggaa actcgatccc cctccgcgtc ggcgtgctcc
     2221 tcatggagcg ccccggcgtc cgcctcctca tcgacaccgg cgtcggcgac gacggcgtcc
     2281 cccgcaacgt cagcggcttc ttcggcggcc tctccgccac cggcggcctc gaccgcgcgc
     2341 tcaacctcct tggctgggag cgggactcga tcacgcatgt gctgctgacg cacctgcaca
     2401 tcgaccacgc cggcggcctc ttcgcgcagg acgaaacgcc gcgattcccc ggggcgaagg
     2461 tcatcgtctc c
//
LOCUS       DASVOD010000735         2351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_673240, whole genome shotgun sequence.
ACCESSION   DASVOD010000735 DASVOD010000000
VERSION     DASVOD010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673240"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /locus_tag="VF720_15815"
     CDS             complement(<1..1979)
                     /locus_tag="VF720_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880879.1"
                     /translation="MNLAGRDMVGDATRGLEKPERSLGFRLGKVGPPVFIVALVIRLV
                     YLVGYQHNPFFADPQMDALYHDRWAAQIARGDWLGSEVFFRAPLYAYFLGAVYFVSDH
                     SYWFVRIVQAVIGALSCVLVARIGRRLFGPVTGFVAGIMAAFLATSVYYEADLLLVVL
                     ETFLGLLALERVLAVAEVGGRPGALAATFAAGLVLGLAAITRPNFLVLAPVFLGYLLV
                     HLRRHGRAVWGAALWLYLAGLAAPISVVTVRNYVVGEDFVPIASQGGLNFYLGNNPSA
                     DGMAALAPEFRPTWYGGVRDATRLAEEGMGRPLKSSEVSDYWFARGLSWVVSRPAAAF
                     GLFVRKFGYFWGAFEIPNNEDFYFFSRYSILFRWPLLFGFGAVTPLALAGLVHAWRRR
                     RFPTVLAAGVTVYSLSIVLFFVCGRFRAPIVPWLVVPAAFLIVDAASLARRHEWRGLG
                     PAALALALALWIVHSDPSQLRAHHTFAESYLRLGIVDAAKGKSDAAEAAYRSAIDADP
                     RFAEGHNNLGVLLMQSNRSREALEEFERAYAIDPDYPRTLNNLAAWYEHEGDLGRARG
                     YIDRALGRKGDEVEILYNAGIILGRQGDFTGSAAHFRRLIEIEPTHQAARLGLGKSLF
                     MAEKYADAGTAFDRVLENDPRHVEALYFLGLSRARL"
     gene            2014..>2351
                     /locus_tag="VF720_15820"
     CDS             2014..>2351
                     /locus_tag="VF720_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HEX7880880.1"
                     /translation="MVENGCWSLRWQAPFGVRAGIIGRRVDGSLVAGAGAPCGPVARV
                     DQVHGVHVVTVDAPGSAGQADALLTTVPGLTLQVRVADCLPVLLAGPGGVAAIHAGWR
                     GAAGGIVTAAL"
BASE COUNT          411 a          791 c          825 g          324 t
ORIGIN      
        1 agccgggccc gcgagaggcc caggaagtag agcgcctcga catggcgcgg atcgttttcg
       61 aggacgcggt cgaaggccgt tccggcatcg gcgtactttt cggccatgaa caggctcttg
      121 ccgaggccga gccgggccgc ctggtgggtc ggctcgattt cgatcaaccg ccggaagtgc
      181 gcggccgatc cggtgaaatc tccctggcgc cccaggatga tgccggcgtt gtagagaatc
      241 tccacctcgt cccccttccg ccccagcgcg cggtcgatgt aaccccgggc ccgtccgaga
      301 tccccctcgt gctcgtacca ggccgccagg ttgttgagcg tgcgtggata gtcgggatcg
      361 atggcatacg cccgctcgaa ttcctccagc gcctcgcgcg aacgattcga ttgcatgagg
      421 aggaccccga ggttgttgtg cccctccgcg aaccgcgggt cggcatcgat ggccgagcgg
      481 taggccgctt cggccgcgtc gctcttcccc ttggcggcat ccacgatccc gagccgcagg
      541 taagattccg cgaacgtgtg gtgggcccgg agctgcgagg gatcgctgtg cacgatccac
      601 agggcaaggg cgagcgccag cgctgccggc ccgaggccgc gccattcgtg gcggcgcgcg
      661 agcgaggcgg catccacgat caggaaggcc gccggcacca ccagccacgg gacgatcggc
      721 gcgcggaacc gtccgcagac gaagaagagg acgatcgaca ggctgtagac cgtgacgccg
      781 gccgccagca ccgtgggaaa ccgccggcga cgccacgcat gcacgagtcc cgccagcgcc
      841 aggggcgtca cggccccgaa cccgaacagg agcggccagc ggaagaggat cgagtagcgg
      901 ctgaagaagt agaagtcttc gttgttcggg atttcgaacg cgccccagaa gtagccgaac
      961 ttccggacga agagcccgaa ggctgccgcg gggcgactga ccacccagct gagaccccgc
     1021 gcgaaccagt agtccgagac ttcggacgac ttgagcggcc gtcccatccc ctcctcggcc
     1081 agacgggtgg catcgcgaac gcccccgtac caggtgggcc ggaattccgg agccagggcg
     1141 gccatgccgt cggccgaggg attgttgccg aggtagaagt tgagcccgcc ttgcgaagcg
     1201 atcggcacga agtcttcacc gaccacgtaa ttgcgcacgg tgacgaccga gatcggcgcc
     1261 gcgaggccgg ccaggtagag ccagagggcg gcgccccaga cggcccgccc gtggcgtcgg
     1321 aggtggacga gcaggtatcc gaggaagacc ggcgccagca ccaggaagtt ggggcgcgtg
     1381 atggccgcga gtccgagcac gagccccgcc gcgaacgtgg cggccagggc gccgggtcgt
     1441 ccacccactt cggccacggc caggacgcgt tccagggcga gcagtcccag gaatgtctcc
     1501 aggacgacga ggaggaggtc ggcctcgtag tagacactgg tggccaggaa cgccgccatg
     1561 atcccggcca cgaacccggt gaccggcccg aaaaggcgcc gtccgatccg cgccaccagc
     1621 acgcacgaca gggctccgat cacggcctgc acgatgcgga cgaaccagta cgaatgatcc
     1681 gacacgaagt agaccgcacc caggaaatag gcgtagaggg gcgcccggaa gaagacctcg
     1741 ctccccagcc agtcgcctcg cgcgatctgg gccgcccagc ggtcgtgata gagcgcatcc
     1801 atctgcggat cggcgaagaa tggattgtgc tggtacccca ccaggtagac gaggcggatc
     1861 accagcgcca cgatgaaaac cggcggcccc acttttccga gccggaaacc gaggctcctt
     1921 tcgggctttt caagccctct ggtagcatcg ccgaccatgt ccctcccggc caggttcatc
     1981 gactcagctc cgcgtcaacg cggcgaacgc ctgatggtgg agaatggatg ctggtcgctg
     2041 cgctggcagg cgccgttcgg agttcgggcg ggaatcatcg gccggcgcgt cgacgggagc
     2101 ctggttgcgg gagccggcgc gccgtgcgga ccggtggccc gggtggacca ggtgcatggc
     2161 gttcacgtcg tcacagtcga cgctcccggt tcggcgggcc aggccgatgc cctcctgacc
     2221 acggtgcccg ggctcacgct ccaggtgcgc gtggccgact gcctccccgt cctcttggcc
     2281 ggcccgggtg gcgtggcggc cattcatgcc gggtggcgcg gcgcggcggg cggcatcgtg
     2341 acggcggcgc t
//
LOCUS       DASVOD010000736         3199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_518228, whole genome shotgun sequence.
ACCESSION   DASVOD010000736 DASVOD010000000
VERSION     DASVOD010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518228"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VF720_15825"
     CDS             complement(<1..999)
                     /locus_tag="VF720_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX7880881.1"
                     /translation="MILVVLDTVRGDRLGCTGFAEARTPHLDSLAAAGVMFADAMTIA
                     PVTLPAVTTVLTGRWPFHHGVRDNDRFVLAEDEETLAERFQAAGWRTGAVLGSTVLDH
                     SRGLGQGFESWDDAFAAPFPVYDPTLRPVAADLATKERRADRVTDLALEKLRQFGSDP
                     AFLLVHYFDAHMFYDPPPRFMAQHPRRPYDAEISFIDAELGRLLADVRRMRPNALVVV
                     VSDHGESNMEHGEPQHGFLVYQSTLRTVAIAAGTGVPRGVVRPDLVSLVDVEPTIARA
                     VGLPAPRVSRDGRALTWGKEEKDAPIQYAESLRPLLSYGWSELRTLRSGQWKWIEGA"
     gene            complement(1472..>3199)
                     /locus_tag="VF720_15830"
     CDS             complement(1472..>3199)
                     /locus_tag="VF720_15830"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7880882.1"
                     /translation="LGVVLYELLVGAHPFDEQRLRHASLLEAWRVIREEDPPKPSTRF
                     RTLAATGTGSTPRPERDRLTTRLRGDLDWITMKALEKDRNRRYESAGDFALDLQRHLR
                     HEPVLATPPGTTYIVSKLVRRHRVPLIAGGVVLLAIIAGGVTSTVLYFKAQRAAAIAR
                     QERDRSDEVATMMKEMLKGAGPSVALGRDTRLLRDLLDTTVRRLEAEGSASPLVRSEM
                     LGVIGATYMDLGEYEAAGPPLETALAIRRTASGERDPEALALTAATGQLQYRLGNMAA
                     AESLLDWSGRAQAEVVGPENEATLSTRVALVETLGYMGKLNEADSLGTPLLATLRRVA
                     GDTAEVTRSAMFALAQTYTDLDRLADAESLYLEQRDILLRTVGPGHPTTLSMAVNLGW
                     LYRLEGRLPEAEAETRTALADMRRLLGNDHAETQVAVNNLGIILSDAGRPREAEAYYI
                     EGLEHGRRVLGDKHPETLASMVNLATFSMKSGHCDRARPLLDEAIASFEETMTDAIGL
                     GIAHSVRAECRLKSGRRADALADFMEARRILSEIFPADHPRLKRVEDGIRAAGGVARR
                     AGQFDRTTP"
BASE COUNT          546 a         1066 c         1083 g          504 t
ORIGIN      
        1 cgctccctcg atccacttcc actggccgga acggagcgtc cgcaattcac tccacccgta
       61 actgaggagc gggcggagcg actccgcgta ctgaatcggc gcatccttct cctcctttcc
      121 ccaggtcaac gcgcggccgt cgcgggatac ccggggcgcg gggagcccga ccgcccgggc
      181 gatcgtcggc tcgacgtcga cgaggctcac gagatcgggg cgtaccactc cccgtggaac
      241 ccccgtcccc gcggcgatcg ccaccgtacg gagtgtggac tggtacacga ggaatccgtg
      301 ctgcggctct ccatgctcca tgttgctctc gccatggtcc gacacgacca ccaccagcgc
      361 attgggccgc attcggcgca cgtcggcaag aagccggccc agctcggcgt cgatgaacga
      421 gatctccgcg tcatacggac gtcggggatg ctgcgccatg aaccggggag gggggtcgta
      481 gaacatgtga gcatcgaagt agtgaacaag gaggaacgcg gggtccgatc cgaactggcg
      541 gagcttctcg agggccaggt cggtcacccg gtcggcgcgc cgctccttcg tggccaggtc
      601 ggccgctacg ggacggagag tcggatcgta gacagggaag ggggcggcaa aggcgtcatc
      661 ccagctctcg aagccctgcc ccaggccccg cgaatgatcg agcacggtgg agccgagaac
      721 cgccccggtg cgccaccccg ccgcctggaa gcgctcggcg agcgtctcct catcctccgc
      781 cagcacaaag cggtcgttgt cccggacccc atgatggaac ggccagcgtc ccgtgagcac
      841 cgtcgtgacg gccgggagag tgaccggcgc gatggtcatg gcatctgcaa acatcacgcc
      901 cgcggccgcc agtgaatcga gatgaggcgt acgggcctcg gcgaaaccgg tgcagcccag
      961 gcggtcgcca cggaccgtgt cgaggacgac gaggatcacg gcatcgggct tcgcggcgga
     1021 ccccgatggt tgcaagccac acgagaagga ggaaaaaatg ctgaataccg tcccggcaac
     1081 gacgacgatt gtccgggtcg gcgacgcgtc gaagaacggc gaacgccatc cgcctgcacg
     1141 gagcatccgt tacacctcac gatcagcggc cattggactg tgattcggcg tatcaacgcc
     1201 gcggcgctcc atgcaggggg ggcaccggtc gaatgagtgc gaggggggga catcgcgact
     1261 cgagcttcac gtgacgggaa tgcaggcggg gacaaccgac ccgtcaacgc agtttccgaa
     1321 ccgacgctat agacgctgcg tgatgtcgat cagttgcggg tgatcatgta aaagttgtgt
     1381 cacagatgtg atgcgcgatg cgtgaagatt acgatgtaaa aagtcatccg atattgttca
     1441 tgcgcggcac cggtatagcg aggaaaaaac gtcatggtgt cgtgcgatcg aactgccccg
     1501 cgcggcgagc gactcccccc gccgcgcgga tgccgtcctc cacccgcttc aggcgcggat
     1561 ggtcggccgg gaagatctcg gacaggattc gtcgggcctc catgaagtcg gcgagcgcgt
     1621 ccgcccgacg tcccgacttg aggcggcact cggcccggac gctgtgggcg atgccgaggc
     1681 cgatcgcgtc ggtcatcgtc tcctcgaacg acgcaatggc ttcatcgagg agcggccggg
     1741 cacgatcgca atggccgctc ttcatgctga aggtggccag gttcaccatg ctggccaagg
     1801 tctcggggtg cttgtcgccg aggacgcgac gaccatgttc gagtccttcg atgtaatagg
     1861 cttccgcctc ccggggccgt cccgcgtcgc tcaggatgat cccgaggttg ttcaccgcca
     1921 cctgcgtttc ggcgtggtcg ttccccagca ggcgacgcat gtcggccagc gcggtgcgcg
     1981 tttcggcctc ggcctcggga aggcgtccct ccagccggta gagccacccc aggttcacgg
     2041 ccatcgagag cgtcgtcgga tgcccgggcc cgaccgtccg aagcaggatg tcgcgctgct
     2101 ccaggtagag cgactcggca tccgccaggc ggtcgaggtc ggtgtaggtc tgcgccaggg
     2161 caaacatggc cgagcgcgtc acctcggccg tgtcgcccgc cacccggcgg agggtggcca
     2221 ggagaggcgt gccgagggag tcggcctcgt tcagtttccc catgtagccc agcgtttcga
     2281 cgagggccac gcgggtcgac aacgttgcct cgttctcggg ccccacgact tcggcctgcg
     2341 cgcgccccga ccagtcgagc agcgattccg cagcggccat gttgcccagg cggtactgaa
     2401 gctgcccggt ggccgcggtc agtgccagcg cctccggatc gcgctccccc gatgcggtgc
     2461 ggcggatggc gagtgccgtc tcgagaggcg ggccggccgc ctcgtactcc ccgaggtcca
     2521 tgtacgtggc gccgatcacc cccagcatct cggagcggac cagcggactg gcactcccct
     2581 cggcctcgag ccggcgaaca gtggtatcga gaaggtcacg cagcagccgg gtatcgcgcc
     2641 ccagagcaac cgatggaccg gcccccttca gcatctcctt catcatggtg gccacttcgt
     2701 cggaacggtc gcgttcctgt cgtgcgatgg ccgccgcgcg ctgcgccttg aaatacagga
     2761 cggtgctcgt caccccaccg gcgatgatgg ccaggagcac gacgcccccc gcgatgagag
     2821 gcacgcgatg tcggcgcacg agcttggaca cgatgtaggt cgtgcccggc ggcgtggcca
     2881 gcaccggctc gtgccgcagg tgccgctgga ggtcgagcgc gaaatcgccg gccgactcat
     2941 atcgacgatt ccgatctttc tcgagcgcct tcatcgtgat ccagtcaagg tcgccgcgca
     3001 gccgcgtggt cagccggtcg cgctcggggc gcggcgtcga tccggtaccc gtggcggcca
     3061 gcgtgcggaa gcgcgtactc ggcttaggcg gatcttcctc gcgaattacg cgccatgcct
     3121 cgaggaggct ggcatgccgg aggcgctgct cgtcgaacgg atgcgcgccg accagcagtt
     3181 cgtagagcac cacgcccag
//
LOCUS       DASVOD010000737         4936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_883272, whole genome shotgun sequence.
ACCESSION   DASVOD010000737 DASVOD010000000
VERSION     DASVOD010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883272"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VF720_15835"
     CDS             <1..994
                     /locus_tag="VF720_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880883.1"
                     /translation="FSTPGLHTLIVKLRDTAGAITTGLVLVRVIPVVRDRPVLLVDDF
                     PVAKRGQVLDADCDERMRRMLAAAGYEPRDIYEWQMWGSGDREIAPILPRLSELTTYK
                     LLIWSVSGTGFNVNPGLIALSSCPGARILRSYLDDGGAIWITGQYAFGAMQSIPQGPV
                     RCGANIAYTEREGIFPGPDDFACRYFNLCGAEFRTTRDRSQMDGLTGAIATPEARLEG
                     FPDLSLDSTLVHPNLSGLVGADALFQPVYDATGGLDTLYTGRQTSPISRFLGKPIAFR
                     YLDPDPGNRTGAVAVTAFPPHYMKPGSAAERTGVAGLGITMINWFKRHERSVDR"
     gene            complement(1059..2174)
                     /locus_tag="VF720_15840"
     CDS             complement(1059..2174)
                     /locus_tag="VF720_15840"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HEX7880884.1"
                     /translation="MHFRDLVSQEEAVERLHAALAGGRLPHAFLFHGRSGVGKTTAAL
                     ALSRRLLCSSPAGFEPCDHCANCSRTAHFNHPDLFLLPPTPAAPDTQAGEGQRMDWIS
                     GVQSDMGKSPIFRLDESKPLEHRIATMRWLKQEASRAAAQSDWKIFILKRAGEMNVES
                     ANAILKLLEEPNPGTLLILGVTRTSDLPETIKSRCALIRFRDLPTTEIVRLLGQRLRA
                     NSNDPADAAAIRLAARVAGGSLTRAAALLEEDVVALRDDAVDILSARYGDPAIHERLD
                     AWLRGKDRSRLHLLLELMLLWEGDLLRTKIGAVDPREGLANADREADLVSAAARLPLD
                     AIERTVRAIEEARSAVHGHGYLPLVLPALAESVARTG"
     gene            2311..4797
                     /locus_tag="VF720_15845"
     CDS             2311..4797
                     /locus_tag="VF720_15845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX7880885.1"
                     /translation="MPHGLRNAARSLALLGLLFLSIAPGVVHAADLSGIVRQEDGAPA
                     GAGWRVVVQSIDASKATVTFTNANGAFTLRELAEGRTGITVYPPAVGEAISDPRAGRM
                     VTLSGGRTADIVLTLAPEGAGTGTFNFAYYVGEYFDNAPTEETSDAVNLPAQGTVNNI
                     NMQLGTGGGSLSGRVTQDAGGAGVSGLIVTAFGIDSDTFSFDRTDEDGYYRVTGIPAD
                     NFVVTAGLAFGDPQSIYVGEYWNNSYSGALASLVEVTEGSDTPNINFSLGLGATISGR
                     VTDEASGNGISNALVALYENTLNISAIELTNEEGFYTVRRLAPGTWKVSANSGDNHLS
                     EFWNDKPSLDAADGVTVTSGSTTPNINFALTNGARVTGTVTDQVTLAPLENLLVIVER
                     SSDGLSRVAITDEAGNYEAMALPAGTYTAYVPEIGEWWDNQSSEENATTFSVAQGQTV
                     ANINFSGILLVADCPLDPQQVGSVAGTVLDAELEPIADATVGLYMDVLGNLFQIGQAT
                     TDINGNYLIDCVSAGDYFVQATAEYTPWLSEWYNNATEENPDAITVEATQLTDNIDFQ
                     LARGGTINGRITGPLGVGLAGVEVHARNRDTENSTTDITDVDGNYSISVGADGGLPGG
                     MYTVWSDGKSTADPTLIPVVLASFTAVAVDGTVRLEWTTSREAWHAGFHVERGAAPDG
                     ASVRISSRLITNGPAYRFVDEAPLAGDAWYWLVSVDRDGRTERLGPLAVGTGTPAVSR
                     LLGPAVNPSRETASIRWEMARPGRLTLQVFDAAGRWVTTLVDDVRPAGAGAVQWDGNT
                     ASGEEAAAGLYFLRFATADGQERGRLALVR"
BASE COUNT          818 a         1643 c         1705 g          770 t
ORIGIN      
        1 cttctcgacc cccggcctcc atacgctgat cgtcaagctc cgcgacacgg cgggggccat
       61 cacgaccggc ctcgtgctgg ttcgggtgat cccggtggtg cgcgatcgcc cggtgctcct
      121 ggtcgacgac ttcccggtgg cgaaacgggg acaggtgctc gatgccgact gcgacgaacg
      181 gatgcggcgg atgctggccg ccgccggcta tgagccgcgc gatatctacg agtggcagat
      241 gtggggaagc ggcgaccggg agatcgcccc catccttccg aggctctccg agttgacgac
      301 ctacaagctg ctgatctggt cggtttccgg tactggcttc aacgtgaatc ccggcctgat
      361 cgcgttgagc tcgtgcccgg gcgcacggat cctgcgcagc tacctcgacg acggcggcgc
      421 catctggatc accggccagt acgcgttcgg cgcgatgcag agcattcctc agggtccggt
      481 gcggtgcggg gcgaacatcg cctacaccga gagggagggg atcttcccgg gcccggatga
      541 ctttgcctgc cggtacttca acctgtgcgg cgccgagttc cgcacgacgc gcgaccgctc
      601 gcagatggac ggcctcaccg gcgccatcgc cacgcccgag gcccggctcg aaggattccc
      661 cgacctctcg ctcgactcga cgctggtgca tcccaacctg tcgggactgg tcggcgcgga
      721 cgctcttttc cagccggtgt acgacgccac cggcgggctc gacaccctct acacgggccg
      781 gcagacgagc ccgatctcac gattcctggg taagcccata gccttccggt atctcgatcc
      841 cgatcccgga aacaggaccg gcgccgtggc ggtgacggcg ttcccgcccc actacatgaa
      901 accgggatcg gcggccgagc gcaccggcgt ggccggcctc ggcatcacca tgatcaactg
      961 gttcaagagg cacgagcgct ccgtcgaccg ctgaccgggg aacaatcgcc cacgtcccga
     1021 cgggacgcga ggcgcgtcgg cgacctgggt tgccgccatc agcccgtccg tgccaccgat
     1081 tcggcgaggg ccggcaggac gagcgggaga taaccgtgcc cgtggacggc cgagcgtgcc
     1141 tcttcaatgg cacggacggt tcgctcgatg gcgtcgagcg ggagccgggc ggccgccgat
     1201 accagatccg cttcccgatc ggcgttggcc agcccctcgc gtggatcgac cgccccgatc
     1261 ttcgtgcgga ggagatcccc ctcccacagg agcatcagct cgagcaggag atggagacgt
     1321 gagcggtcct tgccgcggag ccaggcgtcg agccgctcgt ggatggccgg atcgccgtaa
     1381 cgcgccgaca ggatgtcgac ggcgtcgtcg cgcaacgcca ccacgtcctc ctcgagaagc
     1441 gcagccgcgc gggtgaggct gccgccggcc acgcgggccg ccagccggat tgcggccgcg
     1501 tcggcggggt cgttcgagtt ggcgcgcagc cgttgaccga gcagccgcac gatctcggtg
     1561 gtcggcaggt cgcggaagcg gatgagggcg cagcggctct tgatcgtttc agggaggtcg
     1621 gaggtgcggg tcacgccgag gatgagcagc gttcccgggt tcggctcctc gagcagcttc
     1681 aggatggcgt tggccgactc cacgttcatc tcgccggccc gcttcaggat gaagatcttc
     1741 caatccgatt gtgcggccgc gcgcgacgct tcctgcttga gccatcgcat cgtggcgatg
     1801 cggtgctcca acggcttcga ctcatcgagg cggaagatgg gagatttccc catgtcggac
     1861 tggacaccgg aaatccagtc catcctctgc ccctcgccgg cctgcgtatc cggcgcggcg
     1921 ggagtgggag gcaggaggaa gaggtcggga tggttgaaat gcgcggttct gctgcagttc
     1981 gcacagtggt cacagggctc gaaacccgcc gggctcgagc agaggagccg tcgcgagagg
     2041 gcgagggccg ccgtcgtctt gccgaccccg ctccggccgt gaaacaggaa ggcatggggc
     2101 aaacgcccac ccgcgagggc ggcgtggagg cgctcgacgg cctcttcctg ggaaaccagg
     2161 tcgcggaagt gcatcgtcga aggatagccg accgcgtcac gctccgcacc ccggctcggg
     2221 atcgcgctag aatgtcgccc ggcaagctga ccacccccct ctcgggtcag aagaaacgaa
     2281 tcaactaccg gaacgatgga ggtaaggccc atgccacacg gtttgcgtaa cgcggcgcgc
     2341 tcgctggcgc tccttggatt gctgttcctg tcgattgccc ccggagtcgt ccacgccgcc
     2401 gatctctccg gcatcgtccg ccaggaggat ggcgcgccgg ccggggcggg atggcgggtg
     2461 gtggtgcagt cgatcgacgc ctcgaaggca acggtgacgt tcaccaatgc gaacggcgcg
     2521 ttcaccctgc gcgaactcgc ggaggggcgc acggggatca ccgtgtatcc acccgcggtg
     2581 ggagaggcca tttccgaccc ccgggccgga cggatggtga cgttgtccgg cgggcggacg
     2641 gccgacatcg tgctgacgct tgccccggaa ggcgcgggca ccggcacgtt caacttcgct
     2701 tactacgtgg gcgagtactt cgacaacgcc ccgaccgagg agacctcgga cgcggtgaac
     2761 ctgccggccc agggaaccgt caacaacatc aacatgcagc tcggcaccgg cggcggcagc
     2821 ctttccgggc gcgtgacgca ggacgcgggg ggagcgggcg tctccgggct catcgtgacc
     2881 gcgttcggca tcgactccga caccttttcc tttgatcgta ccgacgagga cggctactac
     2941 cgcgtcaccg gaatcccggc ggacaacttc gtcgtgacgg cgggcctggc cttcggcgat
     3001 ccgcagagca tctatgtcgg ggagtactgg aacaattcgt actccggtgc gctggcctcc
     3061 ctcgtggagg tgaccgaagg gagcgatacg cccaacatca acttctcgct gggcctcggt
     3121 gccaccattt cgggccgcgt caccgacgag gcgagcggca acggcatctc caacgcgctg
     3181 gttgccctgt acgagaacac gctcaacatc agcgccatcg agctcacgaa cgaggaaggg
     3241 ttctacaccg tccggcggct cgctcccggc acctggaagg tcagcgcgaa ctcgggcgac
     3301 aaccatctct ccgaattctg gaacgacaag ccgtctctcg acgcggcgga cggcgtgacg
     3361 gtgacgagcg gttcgacgac gccgaacatc aacttcgcgc tgaccaacgg cgccagggtg
     3421 accggcacgg tcaccgacca ggtgacgctg gcgcccctcg agaacctgct ggtcatcgtg
     3481 gaacgctcca gcgacggatt gagccgggtg gccatcaccg acgaagccgg gaactacgag
     3541 gcgatggcgc tccccgccgg cacctacacc gcctacgtgc cggaaatcgg cgaatggtgg
     3601 gataaccagt cgagcgagga gaacgccacc accttttccg tggcgcaggg ccagaccgtg
     3661 gccaacatca acttcagcgg catcctgctc gtcgcggatt gtccgctgga tccgcagcag
     3721 gtcggttcgg tcgcgggcac cgttctcgat gccgaactgg aaccgatcgc cgatgccacc
     3781 gtgggcctct acatggacgt actcggcaac ctcttccaga tcgggcaggc cacgacggat
     3841 atcaacggca actacctgat cgactgcgtc agtgccggtg actacttcgt gcaggccacg
     3901 gcggaataca ccccctggct ctccgagtgg tacaacaatg caaccgagga aaaccccgac
     3961 gcgatcacgg tcgaggccac gcagctcacg gacaacatcg acttccagct cgcgcggggg
     4021 gggacgatca acggccggat aacgggcccg ctcggcgtcg ggctggccgg cgtggaagtg
     4081 cacgccagga accgtgacac cgagaactcg accaccgaca tcacggacgt ggacggcaac
     4141 tactccatct cggtcggcgc ggatggcggc ctgcctggcg gcatgtacac ggtctggtcc
     4201 gatggcaaat cgacggccga tccgacgctc attccggtgg tgctggcctc gttcacggcg
     4261 gtggccgtgg acggaaccgt gcgcctcgag tggacgacga gccgcgaggc ctggcatgcc
     4321 gggtttcacg tggagcgcgg ggccgcgccg gacggcgcct ccgtgcgcat ttcgtcgcgg
     4381 ctgatcacga acgggccggc ctaccggttc gtggacgagg ctccgctggc cggcgatgcc
     4441 tggtactggc tggtgtccgt cgatcgtgac ggacggacgg aacggctggg gcccctggcc
     4501 gtcggaacgg gcacgcccgc tgtctcccgc ctcctgggtc cggccgtcaa cccctcgcgg
     4561 gaaaccgcct cgatccgctg ggagatggca cgtccgggtc gcctcacgct gcaggtgttc
     4621 gatgccgcgg ggcgctgggt gacgacgctg gtggacgatg tccggccggc cggcgcgggt
     4681 gcggtgcagt gggacggcaa caccgccagt ggcgaggagg ccgcggccgg cctctacttc
     4741 ctccggttcg cgacggccga cggccaggag cgggggcggc tggcgctggt gcgttgaccg
     4801 gaagtcccgg aacgcgtttt cgctgatcga cagtaattga cgatcatcgc ggcggcgggg
     4861 aaacccgccg ccgctttctt gttgcccggg aagggggacg tagtccgggg agcgtggtgg
     4921 gcggaggggt cagccg
//
LOCUS       DASVOD010000738         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_310251, whole genome shotgun sequence.
ACCESSION   DASVOD010000738 DASVOD010000000
VERSION     DASVOD010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310251"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VF720_15850"
     CDS             complement(<1..628)
                     /locus_tag="VF720_15850"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7880886.1"
                     /translation="MNRPDNLIHWIRSLDPDLRAAQLMMVSPDHEQHPEDAADWCIDS
                     GWGGIFLQWRHLREMEWLTAFMDRLREREDDAPPMFVAVDEEGGLVSDLRMITGTAPS
                     PAALGVIDDSMITFQTALAMGDKLRALGFNLNFAPCLDVNSESRNPVIGTRAFGATPQ
                     IVVKHGLATLEGYARAGLVSCVKHFPGHGSTRLDSHVALPILDAPLEVL"
     gene            complement(625..1458)
                     /locus_tag="VF720_15855"
     CDS             complement(625..1458)
                     /locus_tag="VF720_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX7880887.1"
                     /translation="MEEYLRGVVPNEIGHGKADLLEAVKAQAVAARTYALISRGQYEA
                     DGYDLLATVADQVYSGLAAESPIVDQALEETRGVVALFEGQPIVTNYASTCGGHTADR
                     DEVWDKPPLPYLRGISDKGDDGYWCAGSKYFQWQETWTGEELWTIARKALNRDYAYGI
                     TDESQLKNISVTETGPSGRVRKLSFETTTGSYEARGDRIRWVLYRAANAGPLRSTLFT
                     INVDTHKDRVSKLTVDGAGWGHGVGMCQWGAMTRSRKGQDFRKILGDYYRGVQLAALY
                     P"
     gene            complement(1386..>1935)
                     /locus_tag="VF720_15860"
     CDS             complement(1386..>1935)
                     /locus_tag="VF720_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880888.1"
                     /translation="GRRPVNRRVLIALMAILAAGCSKKPHPSTSTEPPVAARPSVHTP
                     VPFANDPLVKEEPNLRVAVVTNAESVELTCEGSFRLTTGNETDAGKRFAAKTPVTVDK
                     SQGQVRARAGSTTITGGLLRLIPDEDDAVQLNKSRFRAGSTSGPALPAASRSSIASRW
                     RNTSAASSPTKSVTARRISSRR"
BASE COUNT          289 a          612 c          680 g          354 t
ORIGIN      
        1 cgaggacttc gaggggtgcg tcgaggatcg ggagcgccac gtggctgtcg aggcgggtgg
       61 aaccgtggcc ggggaaatgc ttcacgcacg ataccaggcc ggcgcgcgca tatccttcga
      121 gcgtggccag tccgtgcttc accacgatct gcggcgtggc gccgaaggct cgggtgccga
      181 tgaccgggtt ccggctctcg ctgttcacgt cgaggcacgg cgcaaagttc aggttgaagc
      241 caagggcgcg gagcttgtcc cccatggcca gggcggtctg gaacgtgatc atgctgtcgt
      301 cgattacgcc gagagctgcg ggcgacgggg cggtgccggt gatcatccgg agatcggaga
      361 cgagaccccc ctcctcatcc accgccacaa acatcggcgg ggcatcatcc tcgcgttccc
      421 gcaggcggtc catgaaggcc gtgagccatt ccatctcgcg aagatgacgc cactggagga
      481 agatgccccc ccaccccgaa tcaatgcacc agtcggcggc atcctcgggg tgttgctcat
      541 ggtccggcga caccatcatg agctgggcgg cccgaaggtc gggatcgagg cttcggatcc
      601 aatggatgag gttgtcgggg cggttcatgg gtagagggcg gcgagctgca cgccgcggta
      661 gtagtcaccg aggatcttcc ggaagtcctg ccccttccgg ctgcgcgtca tggcacccca
      721 ctggcacatc cccacgccgt ggccccagcc agctccgtcg acggtgagct tcgacactcg
      781 gtccttgtgc gtgtcgacgt tgatggtgaa gagcgtgctg cgcagggggc ccgcgttggc
      841 cgcgcggtac aggacccatc ggatgcgatc gccgcgggcc tcgtagctgc cggtggtcgt
      901 ttcgaatgag agcttgcgga cgcgaccgct cgggcccgtt tccgtgaccg agatattctt
      961 gagctgcgat tcatcggtga tgccataggc gtagtcgcga ttgagcgcct tgcgcgcgat
     1021 cgtccagagt tcttcgcccg tccaggtttc ctgccactgg aaatacttgg agcccgcgca
     1081 ccagtaaccg tcatccccct tgtcggagat gccgcgcagg tagggcagcg gcggcttgtc
     1141 ccacacttcg tcgcgatccg cggtgtggcc gccgcacgtg gaagcgtagt tggtgacgat
     1201 cggttgccct tcgaacagcg ccaccacgcc gcgcgtttcc tcgagcgcct ggtccacgat
     1261 cgggctctcc gccgccaggc cgctgtacac ctggtcggcc accgtggcca gcaggtcgta
     1321 gccgtcggct tcgtactgtc cgcgtgagat gagggcatac gtgcgtgccg cgacggcctg
     1381 ggccttcacc gcctcgagga gatccgcctt gccgtgaccg atttcgttgg ggacgacgcc
     1441 gcggaggtat tcctccatcg ggatgcgatt gaggatcgtg acgccgctgg cagcgccggg
     1501 ccggatgtcg aacccgcccg gaagcggctc ttgttcagct ggacagcgtc gtcctcgtcg
     1561 gggatgaggc ggaggaggcc gcccgtgatc gtggtgctgc cggcgcgggc gcgtacctgg
     1621 ccctgtgact tgtcgaccgt gacgggcgtt ttcgcggcga agcgcttgcc ggcgtcggtc
     1681 tcattaccgg tcgtgaggcg gaaagagccc tcgcaggtca gctcgaccga ctccgcgttg
     1741 gtgacgaccg ctacgcggag gttgggctct tccttgacca gcggatcgtt ggcaaacggc
     1801 accggcgtgt ggaccgacgg gcgcgcggcg acgggtggct cggtactcgt ggaggggtgc
     1861 ggtttcttcg aacatcccgc ggccaggatg gccatgagcg cgatgagcac gcggcggttc
     1921 actggccgcc tcccg
//
LOCUS       DASVOD010000739         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_518508, whole genome shotgun sequence.
ACCESSION   DASVOD010000739 DASVOD010000000
VERSION     DASVOD010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518508"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF720_15865"
     CDS             complement(<1..318)
                     /locus_tag="VF720_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880889.1"
                     /translation="MTPQDKSGQKPNSNAHPETRDPEAIRSTAASSARSTISGPAEPA
                     GATGGQDPLVDKFADSGKLAAAMPHNPTKDAEYGDAATDSPQGATAKPPSFKTTGSTL
                     SEDE"
     gene            513..1010
                     /locus_tag="VF720_15870"
     CDS             513..1010
                     /locus_tag="VF720_15870"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880890.1"
                     /translation="MKLIDLVLPEFDQEMATTRKLLERVPTDKGEWKPHPKSFSMGHL
                     AQLISMMPGWITKVITDTELNVVGGPGYSYEATEALVASFDRNVREAREALVAAAESD
                     FDVSWSLKAGETVLFTETRRTVLWQTINHLVHHRGQLSVYLRMVEVPLPSIYGPTADE
                     PWGKA"
     gene            complement(1014..1850)
                     /locus_tag="VF720_15875"
     CDS             complement(1014..1850)
                     /locus_tag="VF720_15875"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX7880891.1"
                     /translation="MNGILTIAKLTLYEARQRRILLTMLMGGTAFVILFGIGFYFIHK
                     DVVQNERVSFIERQTLLNFFIMAGLYAINFMSIMTAVLMSIDTLSGEISSGVMQTLAA
                     KPIHRSSIVIGKWLAHALVLAGYLLLLSGGILTVARALSGITPPNVPAGIFFIYVGGV
                     VVLTLSVAGGTRLSTIANGVVAFGLFGLAFVGGWIEQIGSMTGNTVTRQIGTIVSLFM
                     PSDSMWRLAAWYLQPSISRDVQATPFSSGSVPSPAMVVWTFGYILVALMVGLWMFRRR
                     GL"
     gene            complement(1855..2772)
                     /locus_tag="VF720_15880"
     CDS             complement(1855..2772)
                     /locus_tag="VF720_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880892.1"
                     /translation="MSDRVIETRGLRKQFGRKLAVADLSLSVGRGEVFGFLGPNGAGK
                     TTSLKMLLGLVEPTAGRATVLGAPLGTRSIRAKLGFLPEHFRFHDWLTGRELLRFHGE
                     LLEMPKARLDDRIESLLARVDLLDAAPRKLREYSKGMLQRIGLAQALLNEPEVVFLDE
                     PTSGLDPIGRLLVRDIIRDLKAQGTAVFLNSHLLGEVEATCDRVVFVKQGRAIRDLEL
                     ATGGEDFEVELRFTPMSAALLEGLEQLAGRPAVHDGAVRLRVKNEETIPKIAQWLVEQ
                     GALLYRLDSRRPSLESTFLEVMGQDQRPG"
BASE COUNT          511 a          856 c          982 g          487 t
ORIGIN      
        1 ctcgtcttcg ctcagcgtgc tgccggtcgt cttgaaactc ggtggttttg ccgtcgctcc
       61 ctgcggagag tccgtggcgg catccccgta ctccgcgtct ttcgtcgggt tgtggggcat
      121 tgccgccgcc agcttcccgg aatcggcaaa cttgtccacc aaagggtcct gtccaccggt
      181 ggctccggcg ggctccgccg gaccgctgat cgtggaacgg gcactcgagg cggcggtgga
      241 acgaatggct tcaggatccc tggtctcggg gtgagcattc gagttgggct tctggccgga
      301 cttgtcctgc ggtgtcatgt gagccccacg tgattcgtcg gtgagaattc aaggcgatcc
      361 atgtggctgg tgaagttcgg gtcgcccaac tccaatgaat gagacaacgg gttcatgatt
      421 tccaacagcg gaatagcccc caaacacagg taggtgaggg cccgatcagg taaggttgga
      481 acgggagtcc gacgtgcgcg actggaggaa gcatgaaact catcgatctc gtcctgcccg
      541 aattcgacca ggaaatggcc acgacccgaa agctgctgga acgcgtcccg accgacaagg
      601 gggagtggaa gcctcatccg aagtcgtttt ccatggggca cctggcgcag ctcatctcga
      661 tgatgccggg gtggatcacg aaggtgatca cggatactga gctcaatgtg gttggcgggc
      721 cggggtacag ctacgaagcc actgaggcgc tggtggcgag cttcgatcgc aatgtgcggg
      781 aggcgcgcga ggcgctggtg gcggcggcgg agtcggactt cgacgtctcc tggtcgctga
      841 aggcggggga gacggtgttg ttcacggaga cgcggcggac ggtgctgtgg cagacgatca
      901 accacctggt acaccaccgc gggcagctca gcgtctatct caggatggtc gaggttccgc
      961 tgccttcgat ctacgggccg accgccgacg agccgtgggg gaaggcgtag ggcctacagc
     1021 ccccgccgcc ggaacatcca caacccgacc atgagcgcca cgaggatgta gccgaacgtc
     1081 cacaccacca tcgccgggct cggcacggag cccgaggaga agggcgtggc ctgcacgtcg
     1141 cgcgagatgg agggctggag gtaccaggcg gccaggcgcc acatggagtc gctcggcatg
     1201 aagaggctca cgatggtgcc gatctggcgg gtgaccgtgt tgccggtcat ggagccgatc
     1261 tgctcgatcc agccgccgac gaaggccagg ccgaagagac cgaaggccac gacgccgttg
     1321 gcgatggtgc tcagccgcgt gccgccggcc accgacagcg tgagcaccac gactccgccc
     1381 acataaatga agaagatgcc ggccggcacg ttggggggcg tgatgccgct cagggcgcgg
     1441 gccacggtca ggatgccgcc gctcaacaac aagaggtagc cggccagcac gagcgcgtgg
     1501 gccagccact tgccgatcac gatcgacgag cggtggatcg gcttggcggc cagcgtctgc
     1561 atcacaccgg agctgatttc gccggaaagg gtgtcgatcg acatcaggac ggccgtcatg
     1621 atcgacatga agttgatggc gtagaggccg gccatgatga agaagttgag gagcgtctgg
     1681 cgttcgatga acgacacgcg ctcgttctgc acgacatcct tgtggatgaa gtagaagccg
     1741 attccgaaca ggatgacgaa cgccgtcccc cccatcagca tcgtgaggag gatgcgccgc
     1801 tggcgagcct cgtacagcgt gagtttcgcg atggtcagga ttccgttcat cgtgtcatcc
     1861 ggggcgctgg tcctggccca tcacttcaag gaaagttgat tcgagggagg gtcggcgcga
     1921 atcgagccgg tagaggagtg cgccctgctc cacgagccac tgcgcgatct tcgggatcgt
     1981 ctcctcgttc ttcacgcgca ggcgcaccgc gccgtcgtgc accgcgggtc ggcccgccaa
     2041 ttgctcgagc ccttccagca gggcggcgct cattggcgtg aagcgaagct ccacctcgaa
     2101 atcctcgccg ccggtcgcga gctcgaggtc gcggatggcg cgcccctgct tcacgaacac
     2161 cacgcggtcg caggtggcct ccacttcgcc caggagatgc gagttgagga acacggcagt
     2221 gccctgggcc ttcaggtcgc ggatgatgtc gcgcacgagg aggcggccga tcgggtcgag
     2281 gccggatgtc ggctcgtcga ggaaaaccac ctccggctcg ttcaacagcg cctgtgccag
     2341 gccgatccgc tgcagcatcc ccttgctgta ttcgcgaagc ttgcgcgggg cggcatcgag
     2401 aaggtccacc cgggcgagca ggctttcgat ccggtcgtcg aggcgcgcct tcggcatttc
     2461 aagcagttcg ccatggaagc ggagcagttc gcggccggtg agccagtcgt ggaaccggaa
     2521 gtgctcgggc aggaagccga gtttggcgcg gatggagcgg gtgccgagcg gcgcgccgag
     2581 caccgtggcc cggccggccg tcggttcgac caggccgagg agcatcttga gcgaggttgt
     2641 ttttccggcg ccattcggcc ccaggaagcc gaatacctct ccccgtccga cggacaggga
     2701 gaggtcggcc acggccagct tgcgaccgaa ctgctttcgg agaccgcggg tttcgatgac
     2761 ccggtcgctc atgcgcctcc gccgctcaag gattcggttc tgaagggact gtcactgaag
     2821 attggtggcc atctcg
//
LOCUS       DASVOD010000740         3499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_989827, whole genome shotgun sequence.
ACCESSION   DASVOD010000740 DASVOD010000000
VERSION     DASVOD010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989827"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VF720_15885"
     CDS             complement(<1..265)
                     /locus_tag="VF720_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880893.1"
                     /translation="MKKSISVMWASVMVAGLSLTAAGAFACDGHANKASTEGSTTIMK
                     VSDSGSCASTKASMAGSGCSMKSSMASLQKVAPGTKMSYAKVDG"
     gene            541..627
                     /locus_tag="VF720_15890"
     tRNA            541..627
                     /locus_tag="VF720_15890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:575..577,aa:Leu,seq:caa)
     gene            complement(648..3008)
                     /gene="pbpC"
                     /locus_tag="VF720_15895"
     CDS             complement(648..3008)
                     /gene="pbpC"
                     /locus_tag="VF720_15895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEX7880894.1"
                     /translation="MSRSAIRWLAGLGGVSLILVGILLAQAPAGWSVVASRTAGSLRF
                     EDRSGALLREVPIGGSRARWIALRELPPWVASAVVAAEDHRFACHPGVDPLAIVRAFR
                     DNRRAGRIVSGASTITMQVVRLASGRPPGGSRADKLAEALDALRLEQRLDKDLILEQY
                     LNRAPFGRGTAGIEAASLTWFGHSARTLTPAEATWLAVLPRAPERLSRVDHREELEQR
                     RRLLAARLEATASNRPTPADNPWRAALDPPLVLPRPTPPFEAPHLTSWLLDELPVDIR
                     SRAGTIVTTLDHPLQAEIERRLRPRLERLAEKGARQAAVVVMDNSTRRLAVLLGSVDF
                     FDPEAGQVNGALARRQPGSTLKPFTYALAFESGMTPATLVNDDPHDFAASGGPFAPRN
                     YGGVSFGRVRIRDALAGSLNIAAVDALAHVGADRLFSRLNAMGLLDGRQAADDPGLGL
                     TLGVAEVRLVDLANSYVTLARGGRHAPPVVVTAVRDAAGRRMTLPAAPPERPVIDPLA
                     AWWVADILADDDARLRSFGRNGVLDMPWPVSVKTGTSSDWRDNWAVGFTREWTVAVWV
                     GDFSGAPMHQVSGVFGAAPLLREIFGLLAARGSLTEPVRPKGLDAVVCCLESGDEGTP
                     ACPGTLVEWLPADRRPPACTRHGASADGTTADPALREGSANGSVGDAPSSTRFALRRP
                     ISGDIFFLDPALPRATQAVSFRAEAPAGAEVAWFLDGRPLGTTHAPHERLWRLAPGRH
                     QLIVRIGESERSAAFEVRAGETRPRAAGNAAAAARGADSSEEAVFQ"
     gene            complement(3025..>3499)
                     /locus_tag="VF720_15900"
     CDS             complement(3025..>3499)
                     /locus_tag="VF720_15900"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880895.1"
                     /translation="VEPSVGRPAEESALTLARSWSPFEGGTALTSGDLVLVTLTLVVP
                     EALDFVVMADPLPAGLEPVQLDFRISGEQAARELGRRREGRRAGGLYASWTEMRDREV
                     RVYFDHVPPGVHDFQYLARAVTPGVFGATRARAELMYHPEVAAFTDGPVVRVGSR"
BASE COUNT          554 a         1206 c         1253 g          486 t
ORIGIN      
        1 cgccgtcgac cttggcgtag ctcatcttcg tgccgggggc caccttctga aggctggcca
       61 tcgacgactt catgctgcag ccgctgccgg ccatcgaggc cttcgtcgat gcacacgaac
      121 cactgtcgga caccttcatg atggtcgtgc tgccttccgt ggaggccttg ttcgcgtggc
      181 cgtcacaggc gaaggcgccc gcggcggtca gcgagaggcc ggccaccatg actgacgccc
      241 acatcaccga aatcgacttc ttcatgaatc catcctccct ggcgttgtac tgcggaaact
      301 cgggtatggg ccgcgaagcc cctggaaaac ggcctttgca ggtgctggat caccggcctc
      361 ccccgcgggg ttgtccggtc cggtcatgat actagacgcc gcgtcctcgg gatggacgca
      421 aatttgacgc tccccgagca aggtgatagt tttcgccgcc catcggcccc ggagcttacc
      481 gattgtcaca cggtcggttc cggcgattcc gccgatcgga atttctttac aagtgatcgt
      541 gccggagtgg tgaaactggt agacacacgg gactcaaaat cccgcggggg caactccatg
      601 tcggttcgag tccgacctcc ggcaccatct taattaaatg ccgcaagtca ttgaaatact
      661 gcttcttccg agctgtccgc gcccctggcc gccgccgctg cattgccagc ggcgcgcgga
      721 cgagtctccc ccgcccgcac ctcgaacgcg gccgaacgtt ccgattcccc gatccggacg
      781 atcagttggt ggcgtcccgg ggcgaggcgc cagagccgtt catgaggggc gtgcgtcgtg
      841 cccagcggtc ttccgtccag gaaccacgcc acttcggcac cggccggagc ctcggcgcgg
      901 aagctgacgg cctgcgtcgc ccgtgggagg gcgggatcga ggaagaagat atcgccggag
      961 atcgggcggc gcagggcgaa ccgcgttgat gacggggcgt cgccgacgga tccgtttgcg
     1021 cttccctcgc gaagtgccgg gtcggcggtc gtcccgtcgg cggacgcgcc gtgccgcgtg
     1081 cacgcgggcg gccgacgatc cgccggcaac cactccacca gcgtgccggg acaggcgggc
     1141 gtcccctcgt cgcccgactc gaggcaacag accaccgcat ccagcccctt cggacgcacg
     1201 ggttccgtga gcgacccccg cgcggccagg agaccgaaga tttcccgcaa cagcggcgcg
     1261 gcgccgaaga cacccgacac ctggtgcatc ggcgcgccgc tgaaatcccc cacccagacg
     1321 gcgaccgtcc actcccgggt gaagccgact gcccaattgt cgcgccagtc ggacgaggtg
     1381 ccggtcttca ccgacacggg ccagggcatg tcgagtacgc cgttccggcc gaaggagcgg
     1441 aggcgcgcgt cgtcgtcggc aaggatgtcg gccacccacc aggcggcgag cgggtcgata
     1501 acggggcgct cgggcggcgc ggcggggagc gtcatccgcc ggcccgccgc gtcgcgcacg
     1561 gcggtgacga cgaccggcgg cgcatggcgc ccaccccggg ccagcgtgac gtaggaattg
     1621 gccaggtcga cgaggcgcac ctcggccacg ccgagcgtga ggcccagccc cggatcgtcg
     1681 gcggcctggc ggccatcgag gagtcccatc gcgttgaggc ggctgaagag cctgtcggcc
     1741 cccacgtggg ccagcgcatc caccgcggcg atgttgagcg aaccggccag cgcgtcgcga
     1801 atgcgcacgc ggccgaacga aacaccgcca tagttccgcg gcgcgaaggg gccgcccgac
     1861 gcggcgaagt cgtggggatc gtcgttgacc agcgttgccg gggtcatccc cgactcgaag
     1921 gccagcgcgt aggtgaaggg cttgagtgtc gagccgggct ggcgccgcgc cagcgccccg
     1981 ttcacctggc cggcttccgg gtcgaagaag tcgaccgagc caaggagtac ggcgagacgg
     2041 cgcgtcgaat tatccatgac caccacggcg gcctgccggg cccccttctc ggccagccgt
     2101 tcgagccggg ggcgcaaccg ccgctcgatc tcggcctgca gcggatgatc gagcgtcgtg
     2161 acgatcgtgc ccgcgcgcga gcggatgtcg accggcagct cgtcgaggag ccacgaggtg
     2221 agatgcggcg cttcgaaggg aggcgtcggg cgcggaagga ccaggggtgg atcgagggcg
     2281 gcgcgccatg ggttgtcggc gggcgtcggc cggttcgacg cggttgcttc gagccgcgcg
     2341 gccaggagcc gccgtcgttg ttccagttcc tcgcgatgat ccacgcggga gagtcgctcg
     2401 ggcgcacgcg gcaacaccgc cagccaggtg gcctcggcgg gcgtgagcgt gcgcgccgag
     2461 tggccgaacc aggtcaggct ggccgcttcg atcccggcgg ttccgcggcc gaagggagcc
     2521 cggttcaggt attgctcgag gatcagatcc ttgtcgaggc gttgttcgag ccggagcgca
     2581 tcgagcgcct cggcaagctt gtcggcccgt gaaccgccgg gtggccggcc ggaggccagc
     2641 cgcaccacct gcatggtgat cgtcgacgcg ccggagacga tccggccggc gcggcggttg
     2701 tcccggaagg cgcgcacgat cgcaagagga tcgacacccg ggtggcaggc gaatcgatgg
     2761 tcctcggcgg cgacgaccgc ggaagccacc cagggtggca actcgcggag cgcgatccag
     2821 cgggcgcggc tgccgccgat cgggacctcc cgcaggagag cgccggaccg gtcctcgaag
     2881 cgaagcgaac cggccgtgcg ggaggccacc acactccacc cggccggcgc ctgggcgagg
     2941 aggatgccga cgaggataag gctcacgccg ccgaggcccg ccagccaccg gatcgccgag
     3001 cggctcatcc cgatcttgcc ggggtcatcg cgaaccgacg cgcacgaccg gaccgtcggt
     3061 gaaggccgca acttccgggt ggtacatcag ctcggcgcgg gcccgtgtcg cgccgaagac
     3121 gccgggggtg acggcccggg ccaggtactg gaagtcgtgg acgccggggg ggacgtgatc
     3181 gaagtagacg cgcacctcgc gatcgcgcat ctcggtccag ctcgcgtaga ggcctccggc
     3241 gcgtctcccc tcccggcggc gcccgagctc gcgggccgcc tgctcgccgc tgattcggaa
     3301 gtcgagctgc accggttcga ggccagccgg caacgggtcg gccatcacga cgaagtcgag
     3361 ggcctcgggg acgacaagcg tgagcgtaac gaggacgagg tcacccgacg tgagcgcggt
     3421 gcctccctcg aacggcgacc aggagcgtgc cagcgtgagc gccgactcct cggccgggcg
     3481 gccgaccgac ggctcgacg
//
LOCUS       DASVOD010000741         3564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_934705, whole genome shotgun sequence.
ACCESSION   DASVOD010000741 DASVOD010000000
VERSION     DASVOD010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934705"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..314)
                     /locus_tag="VF720_15905"
     CDS             complement(27..314)
                     /locus_tag="VF720_15905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880896.1"
                     /translation="MKFLFGDSEREETTLHITLMVIGALVLQNLVLVAVLSRLVWPNA
                     IETVRALFLAAMVIMTSPIGEPILLLAGLAALGLIAWVARRPAARGLEVVR"
     gene            complement(311..685)
                     /locus_tag="VF720_15910"
     CDS             complement(311..685)
                     /locus_tag="VF720_15910"
                     /inference="COORDINATES: protein motif:HMM:NF039187.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX7880897.1"
                     /translation="MTSGTRRSGKGGALVGLVLLTLGAVMLLHEQHLFHVGNAWRLWP
                     LFMIVVGLHGLFDSGRRHLLHSLLVVGMGFLFLAINFHLWGLRMRHFAPVVVLIVGLS
                     FVIDAIFGRRKKDEETIDGGVS"
     gene            complement(827..1342)
                     /locus_tag="VF720_15915"
     CDS             complement(827..1342)
                     /locus_tag="VF720_15915"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX7880898.1"
                     /translation="MPDANTPDATAGGLSAARFLSHLGSEAARTRLYLATFEAADMDT
                     PPAAGMMTARELAIHLAACWQWLRMAIVENDPDFKHFQLTATVADGAEAARLYTGEYR
                     KLKEGLSGLGEAGFGRMVKAFGSEMPASDLCLDLLFHDCHHRGQLAAALRAAGRTPPD
                     IYKAGEIPEGE"
     gene            complement(1335..2696)
                     /locus_tag="VF720_15920"
     CDS             complement(1335..2696)
                     /locus_tag="VF720_15920"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX7880899.1"
                     /translation="MDKPRALVVEGDDATAEQLAAFLEERVGLEVVRVKDGEAGYNVL
                     DSSPVNVVVTDLKAHRIDGLRLLEVARARNPEVCVVVIDKQLDLELATEAMRHGAWDF
                     QVKPLNLEKLKAVLDRALSHQRLVTDYSELRERLDHRYGLPSLTGHSREMITVYDRIR
                     QIAPSRATVLIEGETGTGKELVAKALHHASPRRNERFVALNCGALADGVVESELFGHE
                     KGAFTGADQARQGRFELADGGTLFLDEISEVSPSTQLKLLRVLETREFERVGGSTPIA
                     VDVRVIAASNRPLDRAVAEGKFREDLLYRLKVVVIQLPPLRERKSDIPLLADHFLREF
                     NDQNGKSVTGLTRGAMELLMQHEWPGNVRELRNLMEGMVVFGRDGKPLDVGDLPLEMR
                     SNAPAPRTLSVPIGTPMSEVERRVIEETLKAYGHDKPKTAKALDIGLRTLYRKMKEYD
                     LDA"
     gene            2894..3283
                     /locus_tag="VF720_15925"
     CDS             2894..3283
                     /locus_tag="VF720_15925"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX7880900.1"
                     /translation="MTDCKAGPITPLEPAAVDATITGTPGLLVIDFWAPWCPPCVVLL
                     TLIAELVPEFEGRVTFTKFNVDDDSARAVAMGIRGVPTLIFFRDGVEVERLVGTENQC
                     ALRRRLERLVGGASFGKSDSGVCEESE"
     gene            complement(3379..>3564)
                     /locus_tag="VF720_15930"
     CDS             complement(3379..>3564)
                     /locus_tag="VF720_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880901.1"
                     /translation="GLITGGMVFLSQAAVWSSSPLLSGFPSPGVALLVVILGAAFGAC
                     GALMAGRKLGRMEWEVG"
BASE COUNT          615 a         1208 c         1164 g          577 t
ORIGIN      
        1 ctgcccgaag ggcgaacgct gccggttcat cgcaccacct ccagtccacg cgccgccgga
       61 cggcgggcca cccacgcaat gagcccgagg gcggcaaggc cggccaggag caggatcggt
      121 tcaccgatcg gtgaggtcat gatcaccatg gccgccagga agagcgcgcg gaccgtctcg
      181 atcgcgttcg gccacaccag gcgggacagg accgccacca ggacgagatt ctgcaggacg
      241 agggcgccga tgaccatgag cgtgatatgc agcgtcgtct cctcgcgctc ggagtcgccg
      301 aagaggaatt tcacgacaca cctccgtcga tcgtttcctc gtccttcttc cggcgcccga
      361 agatggcgtc gatgacgaag ctcaggccca cgatgaggac caccaccggc gcgaagtgcc
      421 gcatccgaag gccccacagg tggaaattga tggccagaaa gaggaaaccc atcccgacga
      481 ccagcaggct gtgaagcagg tggcggcggc ccgaatcgaa caggccatgc agccccacca
      541 caatcatgaa gaggggccac agccgccagg cgttccccac gtggaagagg tgctgctcgt
      601 gcaggagcat gacggcgccg agcgtgagga gcacgagccc cacgagggcc ccgcccttgc
      661 ccgaccgcct ggttccgctt gtcatcgtga cctcccgttc gtgtgattcg tcgaacgagt
      721 ggaaattacg gcgggaacgc ccctggctcc cttcccgacc ggcgaacggt ggccggctgt
      781 cggtaaacgg cgccaggcga cccgcaagaa tcgcccggcc ggccgcctat tctccttccg
      841 ggatctcccc cgccttgtag atgtcgggcg gcgtgcgtcc ggccgcccgg agcgcggcgg
      901 cgagctgccc gcggtgatgg cagtcatgga agaggagatc gagacagagg tcggaggccg
      961 gcatctcgct gccgaacgcc ttcaccatgc ggccgaaccc ggcctcgccg aggcccgaga
     1021 gtccctcctt cagcttgcgg tactcacccg tgtagaggcg ggcagcctcc gcgccgtcgg
     1081 ccacggtggc ggtgagctgg aagtgcttga agtccggatc gttctcgacg atcgccatgc
     1141 gcagccactg ccagcaggcg gcaagatgga tggccaattc ccgggcggtc atcatgccgg
     1201 ccgccggcgg tgtgtccatg tcggcggctt cgaaggtcgc gaggtagagg cgcgtgcggg
     1261 cggcctcgct acccaggtgg gaaaggaacc gggccgcgga aaggccgccg gcggtcgcgt
     1321 cgggcgtgtt cgcatcaggc atcgaggtcg tactccttca ttttccggta gagggtgcgc
     1381 aggccaatgt ccagggcctt cgcggttttg ggcttgtcgt gaccgtaggc tttcagggtt
     1441 tcctcgatga cgcggcgctc cacctcgctc atcggcgtgc cgatcggcac cgaaagcgtg
     1501 cggggcgccg gcgcgttcga ccgcatctcc agcggcaggt cgcccacgtc gagcggcttg
     1561 ccgtcccggc cgaacaccac catcccttcc atcaggttgc gcagctcgcg cacgttgccc
     1621 ggccactcgt gctgcatgag cagctccatg gccccacggg tcaggccggt caccgacttg
     1681 ccgttctggt cgttgaattc gcggaggaag tggtcggcca ggagcgggat gtcgctcttc
     1741 cgctcacgca agggcggcaa ctggatgacc acgactttga gacgatagag caggtcctcg
     1801 cggaactttc cctcggccac cgcgcgatcg agcgggcgat tggaggcggc gatgacccgg
     1861 acatccaccg cgatcggcgt ggagccaccg acacgctcga attcgcgtgt ttcgagcacc
     1921 cggaggagct tgagctgcgt gctcgggctc acctcgctga tctcatcgag gaagagagtg
     1981 ccgccgtcgg ccagctcgaa acgcccctgg cgggcctgat cggcgccggt gaacgccccc
     2041 ttctcgtgcc cgaagagctc gctctcgacg acgccgtcgg ccagcgcgcc gcaattcagc
     2101 gccacgaacc gctcgttccg gcgcgggctc gcgtgatgga gggccttggc caccagctcc
     2161 ttgccggtgc ccgtttcccc ctcgatcagc accgtggcgc ggctcggcgc gatctgccgg
     2221 atgcggtcgt ataccgtgat catctcgcgg ctgtggccgg tgaggctcgg caggccgtag
     2281 cggtgatcga ggcgttcgcg caattccgag tagtcggtga cgagccgctg gtgactcagc
     2341 gcccggtcga gcaccgcctt caatttctcg agattgagcg gcttcacctg gaagtcccag
     2401 gccccatgac gcatggcttc ggtggccagc tccaggtcga gctgcttgtc gatgacgacc
     2461 acacacacct cgggattgcg ggccctggcc acttcgagga gccggaggcc gtcgatccga
     2521 tgtgctttca ggtcggtgac gaccacgttg accggcgagc tgtcgagaac gttgtagccg
     2581 gcctcgccat ccttcacccg gacgacctcc agtcccaccc gctcttcaag gaaggcggcc
     2641 aactgctcgg cggtggcatc gtctccctcc acgacgaggg cacgtggctt gtccatggtc
     2701 tcgcgggagg gcctcgccaa aatgaaggaa acgattgtag aatcaggaga taggcagtta
     2761 tcttaggtca ggcgcccgaa tccggcaacg ccctggagca tccaagctcc ggccgtcgat
     2821 caaccggcgg gccaccagat ccgacgccac ccccacgtgg ccgtccgcca tcgccacgga
     2881 gcccccgaaa accatgaccg attgcaaagc cggcccgatc acgccgctcg agccggccgc
     2941 cgtcgacgcc acgatcaccg gcacaccggg attgctggtc atcgacttct gggcgccctg
     3001 gtgtccgcct tgtgtggtgc tcctcacgct catcgcggag ctcgtccccg agttcgaggg
     3061 gcgggtgacg ttcaccaagt tcaacgtcga tgacgacagc gcacgggcag tggccatggg
     3121 aatccgcggc gtgcccacgc tgattttctt tcgggatggc gtggaggtcg agcgactggt
     3181 ggggacggag aatcagtgcg cgttacggcg gcggttggag cggttggtgg ggggggccag
     3241 cttcggcaag tcggactccg gcgtctgcga agaatccgag tagcctgcgc tcgagccgcg
     3301 ctcgagtacg tcattccaaa aaaatacgcc gtcattcggt ttactcccct caaggagagt
     3361 tgcggacgat actccgcact accccacctc ccactccatc cgccccagct tccttcccgc
     3421 catcagcgcc ccgcacgccc cgaacgccgc ccccaggatc accactagca gcgccacgcc
     3481 cggcgagggg aaacccgaga gcagcggcga ggaactccac accgcggcct gtgagagaaa
     3541 taccatgccg ccagtgataa ggcc
//
LOCUS       DASVOD010000742         5543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_570438, whole genome shotgun sequence.
ACCESSION   DASVOD010000742 DASVOD010000000
VERSION     DASVOD010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570438"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VF720_15935"
     CDS             <1..789
                     /locus_tag="VF720_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017756707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880902.1"
                     /translation="STPLLELTDLGIQFGGLKAVDGLNLSIASGELIGLIGPNGAGKT
                     TVFNMITGVFPPTSGTALFGGRSLAGLRTHQINRLGIGRTFQNIRLFESMTVAENIRT
                     ALHGHTRNSLVAAALRTPGFRAEEVLLAEEVDRLLETLSLGDVRDEMARSLPYGDQRR
                     LEIARALATRPKLLLLDEPAAGMNPSEKQDLLELIQRIRGPFNLTILMIEHDMRFVMT
                     ICERIAVLDHGIKIAEGVPAAIRQDPKVIEAYLGEDVSHHGAPA"
     gene            786..1505
                     /locus_tag="VF720_15940"
     CDS             786..1505
                     /locus_tag="VF720_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7880903.1"
                     /translation="MSRPLLTVRDLRVSYGGIQALRGVTLDVHEGEVVTLIGANGAGK
                     TTLLRTISGLVRPNSGTVDFVGECDIAREAAHRIVKRGLCHVPEGRIVFANLSVRENL
                     QLGAYLRKDGTEVAKDLEAMYDLFPRLRERMNQSAGTLSGGEQQMLAIARALMGRPRV
                     LLMDEPSLGLAPLLVQEIFRTIREINAKGTTILLVEQNAHMALSVAHRGYVLETGEIV
                     LSGPAVELAADENVRKAYLGL"
     gene            1675..2076
                     /locus_tag="VF720_15945"
     CDS             1675..2076
                     /locus_tag="VF720_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880904.1"
                     /translation="MMTSTFEFLAHPELGYVHIVVHGKMTAPDAASARGRMHTEYPRL
                     NRLWDFRHADLTAWTADDMRMFNETIMMNEGATTKVRIAGLVARDVDFGVARLYESIT
                     KDSFPIEGAVFRDEAQAVAFVTNIAPAGPAD"
     gene            complement(2087..2455)
                     /locus_tag="VF720_15950"
     CDS             complement(2087..2455)
                     /locus_tag="VF720_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018156191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX7880905.1"
                     /translation="MTRKQFEKLVSEAVSALPDEFLAHLGNVDIVVEEFPGPDIIEIE
                     QLESPYDLLGYYEGVPLTERHSSSEPMIPDRITIFQAPIESICNTRAELMEEIQKTVV
                     HEVAHFFGLDEDRISELGWE"
     gene            2526..3467
                     /locus_tag="VF720_15955"
     CDS             2526..3467
                     /locus_tag="VF720_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012309522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase family
                     protein"
                     /protein_id="HEX7880906.1"
                     /translation="MTHSAVAARPAIIVHGGAWGIPDPEVKAHMTGVRAAAEAAWELL
                     DLGGSAIDAVERAILILEDDPTFDAATGSHLNRDGDVELDAGLMEGAELRAGAVAAVK
                     KLKNPILAARKILEMGDHILLVGEGAERYAAEKGVAISPNHVLVVPRELDRWEAQRKG
                     HVKLSTQDTFRHRHFGTVGAVALDRAGQIVAGTSTGGSLYKRPGRVGDSPLPGCGYWA
                     DNELGGVSTTGWGESIIRVGLAKLAADLVGQGAVGPEAAQRAINELGRKVSGWGGLIM
                     IDRAGWVGFAHNTPRMAYAWRTPGMPSVDARIEARAE"
     gene            complement(3630..4454)
                     /locus_tag="VF720_15960"
     CDS             complement(3630..4454)
                     /locus_tag="VF720_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880907.1"
                     /translation="MSPPIPRLEHPIAKLAGFWLFLVAIGFGLALTAAYRVGGIDPGR
                     VLAGSVVSAAGPALLAIVVGWVGAALLYGAATGFDRLVRSFARRLNLLPGGSIETGSD
                     SGSSVVRALLSLAGTWAAFRWLVIPLVVIGRGPTAVPLLAATAGSLLLPITVGFGSEA
                     LLGLSRSVFGRSREWILLRLALRIGWMVFLFLVITGTNLLRGDPLEWATRLGSAGALP
                     GDWLQAATQAVGSLWPVAQVIMVGGLLIQVLGLLRETPGLWRKAPGPDTRTNSTTR"
     gene            4584..>5543
                     /locus_tag="VF720_15965"
     CDS             4584..>5543
                     /locus_tag="VF720_15965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase VI subunit B"
                     /protein_id="HEX7880908.1"
                     /translation="MPKKTSQSQLALLEPEEVAEVKPARASKGVAKTAPKKSEPSAAP
                     KTTARSALKGSATKSEAPPPRPPRVRAPRGSDDEGHSDARAMATRQREISVSEFFSKN
                     RHLLGFDNPAKALLTTIKEAVDNSLDACEEAGILPDLKIEIVELSETRYRVVVEDNGP
                     GIVRQQVPRVFGSLLYGSKFHRLRQSRGQQGIGISAAGMYGQLTTGKPIIIRSKIHPK
                     KPAHHFEIQIDTKKNMPVVGVDKEIEWDVPHGTRVELELEATYKRGKRTVDGYIEQTA
                     LANPHARFVYKAPKDPEVIFERAANELPKAPMEIKPHPHGIELG"
BASE COUNT         1025 a         1873 c         1789 g          856 t
ORIGIN      
        1 agcacgccac tgcttgagct caccgacctc ggcatccagt tcggcgggct caaggccgtg
       61 gacgggctga acctgtccat cgcgtccggc gagctcatcg gtctgatcgg gccgaacggc
      121 gccggcaaga ccacggtctt caacatgatc accggcgtct ttccgccgac gagcggtacg
      181 gcgctcttcg gcggccgttc actggccggc ctcaggacgc accagatcaa ccgcctcggc
      241 atcggccgca cgttccagaa catccgcctc ttcgaaagca tgacggtggc cgaaaacatc
      301 cgcaccgcgc tgcacggcca cacgcggaac agcctggtgg cggcggcgct ccgcacgccg
      361 ggcttccgcg ccgaggaggt gttgctggcg gaagaggtcg atcgcctcct cgaaacgctt
      421 tcgctcggcg acgtccgcga cgaaatggcc aggagccttc cctacggcga ccagcgccgc
      481 ctcgagatcg cccgggccct ggccacgcgg ccgaagctgc tgttgctcga cgagccggcg
      541 gcggggatga acccatccga aaagcaggat ctcctcgagt tgatccaacg gatccgcggc
      601 ccgttcaatc tcaccatatt gatgatcgag cacgacatgc gcttcgtcat gacgatatgc
      661 gagcggatcg cggtcctcga ccatggcatc aagatcgccg agggggtgcc ggcggccatt
      721 cgccaggacc cgaaggtcat cgaggcgtat ctcggcgagg atgtcagcca ccacggagcg
      781 ccggcatgag ccgcccgctc ctcaccgtgc gcgacctgcg cgtgtcgtac ggcgggatcc
      841 aggcgctgcg gggcgtcacg ctcgacgtgc acgaagggga ggtcgtcacc ctcatcggcg
      901 ccaacggtgc cggcaagacc acactcctcc gtacgatctc gggcctcgtc cgccccaatt
      961 ccggaacggt ggacttcgtg ggggagtgcg acatcgcgcg ggaagccgcg caccggatcg
     1021 tgaagcgggg gctctgccat gtcccggagg ggcggatcgt cttcgccaac ctgtcggtgc
     1081 gcgaaaacct gcagctcggc gcctatctgc gcaaggacgg caccgaggtg gcgaaagacc
     1141 tcgaggcgat gtacgacctc tttccgcgcc tgcgggagcg gatgaaccag tcggccggca
     1201 cgctctcggg cggtgagcag cagatgctgg ccatcgcccg tgcgctcatg gggcggccgc
     1261 gggtgctcct catggatgag ccgtcgctcg gcctggcgcc gctgctggtg caggaaattt
     1321 tcaggaccat ccgcgagatc aacgccaagg ggacgaccat tctcctcgtg gagcagaacg
     1381 cccacatggc cctctcggtg gcccatcgcg gttatgtcct cgagaccgga gagattgtcc
     1441 tgtccgggcc cgccgtggaa ctggcggccg atgaaaacgt gagaaaggcg tacctggggc
     1501 tatgacgggc gtcattttcg gtggagcgtc gatctctgtt tggccacggg atggcgcatt
     1561 ttttccgaaa tgacagcgtt atttgagggg tgagtcgagc cgcaatgtaa tagactccaa
     1621 ccgggccgca attctgttgt tccggatcac ctcctcaaga ggtgggagtc cgtcatgatg
     1681 acgtcgacat tcgaattcct ggcgcatccc gagctgggct acgtgcatat cgtcgtccac
     1741 ggaaagatga ccgcgccgga tgccgcttcc gcccgcgggc gcatgcacac cgagtatccc
     1801 cggctgaacc gcctctggga tttccgtcat gcggacctca ccgcatggac cgccgacgac
     1861 atgcgcatgt tcaacgagac gatcatgatg aacgagggcg cgaccacgaa ggtgcgtatt
     1921 gccggcctgg tggcccgcga cgtcgatttc ggcgtggcgc gcctttatga atcaatcacc
     1981 aaggattcat tcccgatcga gggcgcggtc ttccgtgatg aagcgcaggc cgtggcgttc
     2041 gtgacgaaca tcgcgccggc cggaccggcg gactgaccca cttccgctac tcccacccca
     2101 gttccgaaat tcgatcctcg tcgagcccga agaagtgagc cacctcgtgc accaccgtct
     2161 tctggatctc ctccatgagc tccgcgcgcg tgttgcagat cgattcgatc ggcgcctgga
     2221 aaatggtgat gcggtcggga atcatcggtt cgctcgatga gtgcctctcg gtgagcggca
     2281 ccccctcgta gtaacccagg agatcgtagg gcgattcgag ctgctcgatc tcgatgatgt
     2341 cggggcccgg gaactcctcg acgacgatgt ccacgttccc gaggtgggcc aggaattcgt
     2401 ccggcagggc gctcacggcc tcgctgacga gcttttcgaa ctgctttcgg gtcattcccg
     2461 cctcggttat gatgcgcctt cttccagcct ctttttccag tcgaacgagt ctagcagagg
     2521 atctgatgac gcattccgcc gtcgcggccc gtcccgccat catcgtccac ggcggcgcgt
     2581 ggggaattcc cgaccccgag gtcaaggcgc acatgacggg cgtccgcgcc gccgccgagg
     2641 ccgcctggga acttctcgac cttggtggct ccgccatcga cgccgtcgag cgggccatcc
     2701 tgatcctcga agacgatccc accttcgatg cggctaccgg ctcccacctg aaccgtgacg
     2761 gggacgtgga gctcgacgcc ggcctcatgg aaggcgccga actgcgcgcc ggtgccgtgg
     2821 cggcagtcaa gaaattgaag aacccgatcc tggccgcccg caagatcctc gaaatggggg
     2881 atcacatcct cctcgtcggc gaaggcgccg agcgctacgc cgcggagaag ggagtggcca
     2941 tctcgccgaa ccacgttctg gtggtcccac gcgaactcga ccgctgggaa gcgcagcgga
     3001 aggggcacgt gaagctctcc acgcaggaca ccttccgtca ccgtcacttc ggcaccgtgg
     3061 gcgccgtggc cctcgaccgg gccggccaga tcgtggccgg cacctccacc ggcggctcgc
     3121 tctacaagcg ccccggccgc gtcggcgact cgccgctccc cgggtgcggc tactgggccg
     3181 acaatgaact ggggggcgtt tccaccactg gctggggcga gtcgatcatc cgcgtggggc
     3241 tggccaagct ggccgccgac ctggtgggac agggcgcggt gggccctgaa gccgcccagc
     3301 gcgccatcaa cgaactgggt cgcaaggtct ccggatgggg cggactcatc atgatcgacc
     3361 gggccggctg ggtcggcttc gcccacaaca cgccccgcat ggcctacgcc tggcgcacgc
     3421 cggggatgcc gtcggtcgat gcgcggatcg aggccagggc ggagtagtcc gcgctcctgc
     3481 ggttcgggaa gcacgtgccg actttgtgaa aactggcgcg actcgtttct gaaacgagtt
     3541 cgtcaaccgg caactcgttt ctgaaacgag tccggcacca aacaagtccg gcaatcaagc
     3601 gagttccgca atctaatttc gagatagtgt taccgggtcg tggaattcgt acgcgtgtcc
     3661 gggcctggag ccttgcgcca taaccccggc gtctcgcgca gcaggccaag aacctggatc
     3721 aggagcccgc ccaccattat cacctgcgcc accggccaga gcgagccgac cgcctgggtt
     3781 gccgcctgca gccaatcgcc cggcagggca cccgcggaac cgagccgggt cgcccattcg
     3841 agcgggtcgc cgcggaggag gttcgtgccg gtgatgacga ggaagaggaa caccatccag
     3901 ccgatgcgga gcgcgagccg aaggaggatc cattcgcgcg agcgcccgaa gacggaacgg
     3961 gagagtccaa ggagcgcctc acttccgaag ccgacggtga tcggcaggag gagcgacccg
     4021 gccgtagcag ccagaagggg gaccgctgtc ggcccccgcc cgatcaccac gagcgggatc
     4081 acaagccatc gaaacgcggc ccaggtgccg gccagcgaca ggagcgctcg caccaccgac
     4141 gaccctgagt cggatccagt ttcgatcgat ccgccgggca ggagattgag tcggcgggca
     4201 aacgatcgaa cgagccgatc gaatccagtg gcggctccgt agaggagggc cgcgcccacc
     4261 cacccaacca cgatcgcgag tagcgccggc cccgccgccg agacgaccga ccccgcgagg
     4321 acccgccccg gatcgatccc cccgacgcgg tatgccgccg tcagcgccaa tccgaatccg
     4381 atcgccacga ggaacaacca gaagccggcg agtttcgcga tggggtgctc gagacggggg
     4441 atcggcggcg acatgggcgg cattctggca aatccggcgc gggctgtcgc ggcgttgaca
     4501 tccttgacgc ttcgcgccgc cagaacgcag aatggcgcga ccacaacaac ttaccggccc
     4561 acgcggccct ggagcccaca ccgttgccaa agaaaacgag ccagtcccag ctcgcgctgc
     4621 tcgagccgga ggaagttgcc gaagtgaagc cggccagggc ctcgaagggg gttgcgaaaa
     4681 cggccccgaa gaagtccgag ccgtcggcgg ccccgaaaac cacggcaagg tccgctctaa
     4741 agggctccgc caccaagtcc gaggcgccgc cccccaggcc tcccagggtc cgcgcgccgc
     4801 gcggctccga cgacgagggt cactccgatg cccgcgccat ggctacccgc cagcgcgaga
     4861 tctcggtcag cgagttcttt tccaagaatc gccacctcct cggcttcgac aacccggcca
     4921 aggcgctcct gaccaccatc aaggaagcgg tggacaactc gctcgatgcc tgcgaggaag
     4981 ccggcattct tcccgatctc aagatcgaga tcgtggagct ctccgaaacc cgctaccggg
     5041 tggtcgtgga agacaacggc cccggcatcg tccgccagca ggtgccgcgt gtcttcggca
     5101 gcctcctgta cggctccaag ttccatcgcc tccggcagag ccgcggccag caggggatcg
     5161 gcatctcggc ggcggggatg tacggccagc tcaccaccgg caagccgatc atcatccgca
     5221 gcaagatcca cccgaagaag cctgcgcacc acttcgagat ccagatcgac acgaagaaga
     5281 acatgccggt ggtgggcgtg gacaaggaaa tcgagtggga cgtgccgcac gggacgcgcg
     5341 tcgagctgga gttggaagcc acctacaagc gcggcaagcg cacggtggac ggctacatcg
     5401 agcagaccgc cctggccaac ccgcacgcgc gcttcgtcta caaggcgccc aaggatcccg
     5461 aagtcatctt cgagcgggcg gccaacgagt tgccgaaggc ccccatggag atcaagcccc
     5521 acccgcacgg catcgagctg ggc
//
LOCUS       DASVOD010000743         5146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_467630, whole genome shotgun sequence.
ACCESSION   DASVOD010000743 DASVOD010000000
VERSION     DASVOD010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467630"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /gene="secG"
                     /locus_tag="VF720_15970"
     CDS             complement(<1..384)
                     /gene="secG"
                     /locus_tag="VF720_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006115295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX7880909.1"
                     /translation="MLTIALSILFVLVCFLLVVVVLLQTGKGGGVSGAFGIGQASQTI
                     FGASGAGNVLTKATAVLGGAFMLISLLLAMIEGGSGPATGGRSILQEGLSTAPIESPA
                     PAPVSGGTDVPVTTTPAPGSQPATNP"
     gene            complement(457..1215)
                     /gene="tpiA"
                     /locus_tag="VF720_15975"
     CDS             complement(457..1215)
                     /gene="tpiA"
                     /locus_tag="VF720_15975"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX7880910.1"
                     /translation="MTPRRPLVAGNVKMFLTTPAMRQLGADVLAGLGKGEVAEVAYFP
                     PFTMLADAGRLFAGTPIGLGAQNVYWENEGAFTGEVSARMLLDVGCRWVLIGHSERRQ
                     HFGETDATVSKRIGAALRHELKPLVCIGETLAERESGATERVLARQLEGALAGLSDDD
                     CLRLTLAYEPVWAIGTGKVATPEQAEEAHRAIRGNLESRRAGLGEATRILYGGSVKPD
                     NAAGLLSRPGIDGALVGGACLSPASFLEIIRSAG"
     gene            complement(1235..2434)
                     /locus_tag="VF720_15980"
     CDS             complement(1235..2434)
                     /locus_tag="VF720_15980"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX7880911.1"
                     /translation="MNRLTVRDLGVSGKRIFVRVDFNCPLDDDGEVADDTRIVAAIPT
                     LRYLAEKGGRLVIASHLGRPKGGAESRYSLAPVARRLQHLLKTNVAFAADCIGPAVAK
                     ASADLADGQFLLLENLRFHAGEEANDPAFAEALAASADVYVNDAFGTAHRAHASTAGI
                     TRHLQPAAAGFLMEKELRYLGRAVTDPKRPFWAILGGAKISGKIDVIDHLFDKVDGLL
                     VGGGMAFTFFQAAGRSIGRSLLEADKVELARALLARAESKGVPLVLPVDLVVARGPKG
                     ADEHRTVDGVDVPDGWMGVDIGPRTVEEFRKRLGGAGTILWNGPMGIFEEPPYDQGTL
                     AVARLLAEATKAGATTIVGGGDSAAAIANAELEDQVTHVSTGGGASLEFLEGRELPGV
                     AALTPKP"
     gene            complement(2440..3444)
                     /gene="gap"
                     /locus_tag="VF720_15985"
     CDS             complement(2440..3444)
                     /gene="gap"
                     /locus_tag="VF720_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009331817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX7880912.1"
                     /translation="MAIRIGINGFGRIGRLVYRAALPHRDIEIVAINDVTDAPTLAHL
                     LKHDSVHGPLRVPVTVDGSVMSVDGRKTQVLATKDPAHLPWKDLGVSLVVESTGLFTD
                     REKAAAHLAAGAKKVIVSAPGKDADATLVLGVNHASYDPGKHHVVSNASCTTNCLAPV
                     VKVLHDAFGIQRGVMTTIHAYTNDQRILDLPHKDLRRARAAALSQIPSSTGAAKAIGL
                     VVPELKGKLDGSSIRVPVPDGSLVDLTAQVSRNTTAEEVNAALKTAADGPMRGVLEYS
                     TEELVSSDVIGNPHSSVVDSKLTTVMDGNLVKVFSWYDNEWGFSVRVVELIQLMARSL
                     "
     gene            complement(3493..4539)
                     /locus_tag="VF720_15990"
     CDS             complement(3493..4539)
                     /locus_tag="VF720_15990"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX7880913.1"
                     /translation="MLKLGVLTGGGDCPGLNAVIRAVVQATIKRHDGRVFGIHDGWQG
                     MVEGTVAELTLDSVSGILPKGGTILGTSRTNPTRNEAAVESCLKTFRHWNLDALVAIG
                     GEDTLGVALELSRRGMPVVGVPKTIDNDLNGTDYTFGFDTAINIATEAIDRLHTTAES
                     HNRVIVVEVMGRHAGWIAVEAGIAGGADVVLIPEVPISVEEVCATINRRHQRGKKFSI
                     VVVAEGARFEEAKGDDGMLILSDSEKDAFGHVKLGGIGKLLADAIEDKTGFETRYSVL
                     GHIQRGGTPTAFDRVLATRFGVAAVELVVQRQYGRMVALSGNRIIALDIAEAVRDLKT
                     VDMGLYEIAKVFFG"
     gene            complement(4553..>5146)
                     /locus_tag="VF720_15995"
     CDS             complement(4553..>5146)
                     /locus_tag="VF720_15995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEX7880914.1"
                     /translation="EGSWARTEEEVLAEARAVLPEDEYERFKEMPFGDRESQLATFWA
                     SQDPSANTAANERRDTFQERVAFADRNFTTPLERGMTSDRGRVYIRYGPPDEVTREDI
                     PRPGHTLADVLDGGDFSDDLYSDKTDQSRRDGLTSANFQNVDTRPYEIWAYTTSGQPL
                     FPERERLSKNTGLTFIFVDDQGIGVYTLRYSNTPRRF"
BASE COUNT          814 a         1695 c         1769 g          868 t
ORIGIN      
        1 cgggttcgtc gccggctgac tgcccggcgc cggggtggtg gtgaccggga catcggtgcc
       61 gccggagacc ggcgcggggg ccggtgactc gatcggcgcc gtgctcagcc cttcctgcag
      121 gatgctgcgg ccgccggtag cggggcccga cccaccttcg atcatggcaa ggaggagcga
      181 gatcagcatg aaggcgccac ccaggaccgc ggtggccttc gtgaggacgt taccggcacc
      241 cgaggcaccg aagatggtct ggctggcctg gccgatgccg aaggctcccg agacgccccc
      301 acccttgccg gtctgaagga ggacaaccac gacaagcagg aagcacacga gcacgaacag
      361 gatggacagg gcgatggtca gcattcattc ctccggtcgg tacgaccgac ataagtcaaa
      421 caggcgggag agattaccag cgtgaatggg ggcctgtcaa ccggcggaac gaatgatttc
      481 gaggaacgag gcgggtgaga ggcaggcccc gcccaccagc gctccgtcga tccccgggcg
      541 cgacaggagg cctgccgcgt tgtccggttt gacgctcccg ccgtagagga tccgcgtggc
      601 ctcgccgagg ccggcccgac ggctttccag attcccgcgg atggcgcggt gcgcctcctc
      661 ggcctgctcg ggagtggcca ccttgcccgt accgatggcc caaacgggct cgtaagccag
      721 cgtcagtcgg aggcagtcgt cgtccgacag accggccaac gccccctcga gctggcgtgc
      781 gagtacgcgc tcggtggcgc cggactcacg ctcggcaagc gtctcgccga tgcagaccag
      841 cggcttgagc tcatgccgca gcgcggcgcc gattcgtttc gaaaccgtgg catcggtctc
      901 gccgaagtgc tgtctccgct cggagtgccc tatcaggacc caccggcagc cgacgtccag
      961 cagcatccgc gcggagacct ctcccgtgaa cgccccttcg ttttcccagt agacgttctg
     1021 cgcaccaagc ccgatgggtg ttccggcgaa gagtcgcccc gcgtcggcca gcatcgtgaa
     1081 cggcgggaag taggccacct cggcgacctc ccccttcccg aggccggcaa gcacgtcggc
     1141 gccgagctgt cgcatggcgg gagtggtcag gaacatcttc acgttcccgg ccaccagtgg
     1201 tcgtcgcggc gtcatggctt catcacgatg ggggtcatgg cttcggcgtg agcgcggcca
     1261 cgccgggcag ctcacgcccc tcgaggaatt cgagcgaggc gccgccgccg gtcgagacgt
     1321 gggtcacctg gtcctcgagc tcggcgttcg cgatggccgc cgcggaatcg cccccgccca
     1381 cgatggtggt ggcgccggct ttcgtggcct cggccaggag acgggccacc gccagcgttc
     1441 cctggtcgta cggcggctcc tcgaagattc ccatcggacc gttccagagg atggtgccgg
     1501 cgccgccgag gcgcttgcga aattcttcga cggtgcgcgg gccgatgtcg acacccatcc
     1561 agccatcggg gacatccacg ccgtcgaccg tgcggtgctc gtcggctcct ttcgggccac
     1621 gcgcgacgac caggtcaacc gggaggacga gtggcacgcc cttcgattcg gcccgggcca
     1681 ggagggccct ggccagctcc accttgtcgg cttcgaggag cgacctgcca atggagcggc
     1741 cggccgcctg gaagaaggtg aatgccattc ccccgccgac gaggaggccg tccaccttgt
     1801 cgaacaggtg atcgatgacg tcgatcttcc cggaaatctt ggcgccgccg aggatggccc
     1861 agaacggccg cttcggatcg gtgacggcac gaccaaggta gcgcagctcc ttctccatga
     1921 ggaaaccggc ggcggcaggc tggagatgcc tggtaatgcc ggcggtggag gcgtgcgccc
     1981 gatgggcggt gccgaaagcg tcgttcacgt acacgtctgc cgaggccgcc agagcctcgg
     2041 caaacgccgg gtcgttggcc tcctcgccgg cgtggaagcg aaggttttcg agcaggagaa
     2101 actggccgtc ggccagatcg gcggaagcct tcgcgacggc gggcccgatg caatccgcgg
     2161 caaacgccac gtttgtcttg aggagatgct gaagacgccg cgccaccggg gccagcgagt
     2221 agcgcgattc cgcccctccc ttcggccgcc cgagatggct ggcgatgacc agccgtcccc
     2281 ccttctccgc gaggtatcgg agcgtgggga ttgcggccac gatgcgcgtg tcgtcggcca
     2341 cttccccgtc gtcatcgagc ggacagttga agtcgacgcg gacgaagatc ctcttccccg
     2401 aaacgccgag atcgcggacg gtgagccggt tcatcgcgct cagagggagc gcgccatgag
     2461 ctggatcagc tcgaccacgc gcaccgagaa gccccactcg ttgtcgtacc aggagaagac
     2521 cttgacgagg ttgccgtcca tcaccgtggt gagcttcgaa tcgaccacgg acgaatgcgg
     2581 gttgccgatg acgtcgctcg agacgagttc ttcggtggag tactccagga cgccccgcat
     2641 cgggccgtcg gccgcggtct tgagagccgc attgacttcc tcggccgtcg tgttgcgact
     2701 tacctgggcc gtgaggtcga ccaacgagcc gtcgggcacg gggacgcgga tggagcttcc
     2761 gtcgagcttg cccttgagtt cggggacgac gaggccgatg gccttggccg cgccggtgga
     2821 cgacgggatc tgcgaaaggg ccgcggcccg cgcccggcgc aggtccttgt gcggcaggtc
     2881 gaggatgcgc tggtcattgg tgtaggcatg gatggtggtc atcacgccgc gctggatccc
     2941 gaacgcgtcg tggagcacct tgacgaccgg cgcgaggcag ttggtggtgc aggaggcgtt
     3001 ggagacgacg tgatgcttcc ccgggtcgta gctcgcatga ttgacgccca ggacgagggt
     3061 ggcgtcggca tccttgcccg gcgccgacac gatcactttc ttcgcgccgg ctgccaggtg
     3121 ggcggcggcc ttttcgcggt cggtgaagag gccggtcgac tcgaccacca gcgagacgcc
     3181 gaggtccttc caggggagat gagccgggtc cttcgtggcc agcacctggg tcttccgtcc
     3241 atcgacgctc atgaccgaac cgtccaccgt gaccggcacc cgaagcggac cgtggaccga
     3301 gtcgtgcttc aggaggtgcg ccagcgtggg cgcgtcggtc acgtcgttga tcgccacgat
     3361 ctcgatgtcg cggtgcggca atgccgcccg gtagacgagg cgaccgatgc gcccgaagcc
     3421 gttgatgccg atccggatgg ccatggtttc tccttcaggg attcgggccc gacgcgcggg
     3481 ccacggggtt ggtcagccga aaaagacctt tgctatctcg tacagcccca tgtcgaccgt
     3541 tttcaggtcg cgcaccgcct cggcgatgtc gagggcgatg atccggttac ccgaaagggc
     3601 caccatcctg ccgtactggc gttgcacgac cagctccacg gccgccacgc cgaagcgggt
     3661 ggccaggaca cgatcgaacg cggtgggcgt accgcctcgc tggatgtggc cgagcacgga
     3721 gtatcgcgtt tcgaagccgg tcttgtcctc gatggcatcg gccagcagct tgccgatgcc
     3781 gccgagcttg acgtggccga aggcatcctt ctccgagtcg gagaggatga gcattccgtc
     3841 gtcccccttg gcttcctcga agcgggctcc ctcggccacc accacgatgc tgaacttctt
     3901 gcctcgctgg tgacggcgat tgatggtggc gcagacctcc tccaccgaaa tgggaacctc
     3961 cgggatcagc accacatccg ctccgccggc gatgccggcc tcgaccgcga tccagcccgc
     4021 gtgccgaccc atcacctcca ccacgatgac gcggttgtgc gactcggcgg tggtgtgcag
     4081 ccggtcgatg gcctcggtgg cgatgttgat ggccgtgtcg aagccgaacg tgtaatcggt
     4141 gccgttcagg tcgttgtcga tcgtcttggg gacgcccacg accggcatgc cgcggcgcga
     4201 caactcgagc gccacgccca gggtgtcctc gccgccgatg gccaccaggg catccaggtt
     4261 ccagtgccgg aacgtcttga ggcagctttc gacggcggcc tcgttccggg tggggttggt
     4321 gcgggaggtg cccaagatgg tgcccccctt gggaaggatg ccggataccg agtcgagggt
     4381 gagctcggcg accgtccctt ccaccatccc ctgccagccg tcgtggatgc cgaagacgcg
     4441 cccgtcatgc cgcttgatgg tggcttggac gactgcccgg atgaccgcat tgaggcccgg
     4501 gcaatcgccg cctcctgtca gtacccccag cttgagcatg ggggcgacga tatcagaagc
     4561 ggcggggggt gttcgagtag cgcagcgtat acacgccgat cccctggtcg tccacgaaaa
     4621 tgaacgtgag gccggtattt ttcgagagcc gctctcgctc cggaaacagc ggctggccgc
     4681 tggtcgtgta ggcccagatc tcgtaggggc gggtatcgac gttctggaag ttggcgctgg
     4741 tcaggccgtc gcggcggctc tggtcggtct tgtcgctgta gaggtcgtcg ctgaagtcgc
     4801 cgccgtccag cacgtcggcc agggtatgcc cggggcgcgg gatgtcctcg cgggtcacct
     4861 cgtccggggg gccgtaccgg atgtacaccc gtccccggtc gctggtcatc ccacgctcga
     4921 gaggcgtggt gaaattccgg tcggcgaaag ccacccgttc ctggaaggta tcccgccgtt
     4981 cgttggccgc cgtattggcc gagggatcct gggacgccca gaacgtggcc agctggctct
     5041 cgcgatcacc gaagggcatt tccttgaagc gctcgtactc gtcttccgga aggaccgcgc
     5101 gcgcttcggc cagcacttct tcctcggtcc tggcccacga tccctc
//
LOCUS       DASVOD010000744         2474 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_934936, whole genome shotgun sequence.
ACCESSION   DASVOD010000744 DASVOD010000000
VERSION     DASVOD010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934936"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VF720_16000"
     CDS             <1..1810
                     /locus_tag="VF720_16000"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX7880915.1"
                     /translation="TAGFRETGGAGIVRETELKELIRRYGMRMVGPNCMGVINTEAGV
                     RLDASFARTRPIQGDIGFMSQSGALGEAILSIAGRKGIGFSMFVSVGNKTDVSGNDLL
                     SYWENDPATRVILLYLENFGDPRRFTTIARRITRSKPIIAVKAGRTSAGARAVSSHTG
                     SLAGRDLATDLLFEQCGVIRAASVEELFDLAPAFSLQPPPTGPRVAIVTNAGGPGILA
                     TDACVGHGLLVESPSAETREFLASRISPEASLENPIDLLASATPEMYGAGVRAALEDP
                     AFDALIVIFVPPVMTRSDEVAAAIAGAAAGAGKPVLVCFMGADEGVPGVAELRAGGIP
                     VYTFPESAANALAALEMYGRRRVRPDGQPVTMPIDHPRAARAIEAARRDGREWLSGSE
                     AREVVAAAGLPLARAVSIVTRDDCGTAADRLTFPMALKRDGSGSEHKSDEGGVRLGIA
                     SIQELHAAFDALGGGATPLLAQEMISGGRELILGMTVDPIAGPLLMAGTGGTEVELWR
                     DVVWRIHPLTDLDAGDMLRAMTGFPRLSGYRGGAPVDIAGIEGDLLRLSSLVGEFPEI
                     AELDINPYIARPAGAGNRIVDVRVRLAPQISSGGAS"
     gene            complement(1783..2046)
                     /locus_tag="VF720_16005"
     CDS             complement(1783..2046)
                     /locus_tag="VF720_16005"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX7880916.1"
                     /translation="MFNCDDVIQLLTDYVDGELEPETQRLLDTHFGRCPHCDQFLRSF
                     KVTVELTGEFRCEDIPQEVSHRLHEFLEQRLRTCDQEAPPEEI"
     gene            complement(2062..>2474)
                     /locus_tag="VF720_16010"
     CDS             complement(2062..>2474)
                     /locus_tag="VF720_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX7880917.1"
                     /translation="KIHTFRGHAAFSSWLYRITANAAFARLNQRKRHAAVSLDDVLPV
                     IEEAGADDGSSEWAQKPDLALFNKETREALESAIQALPDDFKTVVVLRDIQNLSNQEV
                     ADILNLSIPAVKSRLHRARLALRRKLGDYLGGPA"
BASE COUNT          371 a          803 c          884 g          416 t
ORIGIN      
        1 caccgccggc tttcgcgaga ccggcggtgc ggggatcgtc cgcgagaccg agttgaagga
       61 actcatccgg cgatacggca tgcggatggt cggacccaac tgcatgggcg tgatcaacac
      121 cgaggccgga gtgcgtctcg atgccagctt tgcccggacg cgccccatcc agggggacat
      181 cggcttcatg agccagagcg gcgcgctcgg ggaggccatt ctctccatcg ccggcaggaa
      241 ggggatcggc ttctcgatgt tcgtctcggt cgggaacaag accgacgtgt cgggaaacga
      301 cctcctctcc tactgggaga acgatccggc cacgcgcgtc atcctcctct atctggagaa
      361 cttcggcgat ccgcgccgct tcaccacgat cgcgcggcgc atcacccggt cgaagccgat
      421 catcgcggtg aaagccgggc gcacctccgc cggcgcgcgg gcggtttcga gccacaccgg
      481 ctcgctggcc ggacgcgacc tggccaccga tctgttgttc gagcagtgcg gcgtgatccg
      541 cgccgcctcg gtcgaggaac tgttcgacct ggcgccggcc ttctccctgc agccgccccc
      601 gaccggacca cgcgtggcga tcgtgaccaa cgcggggggt ccggggatcc tggccaccga
      661 tgcctgcgtc gggcacgggc tgctggtcga gtcgccttcg gcagagacgc gggagttcct
      721 ggcctcacgg atttcgcccg aggcgagcct ggagaacccg atcgaccttc tggccagcgc
      781 cacgccggag atgtatggcg cgggagtccg ggcggcgctc gaggatccgg cattcgatgc
      841 attgatcgtg atcttcgtgc cgccggtcat gacgcgctcg gacgaagtgg ccgccgccat
      901 cgccggcgcg gcggccggcg ccggaaagcc cgtcctggtc tgtttcatgg gcgccgacga
      961 gggtgtgccc ggcgtggccg agctccgggc gggcggcatc ccggtctaca cctttcccga
     1021 gtcggccgcg aatgcgctcg cggcgttgga gatgtacggc cgccggcgcg ttcgccccga
     1081 cggacagccc gtgacgatgc cgatcgatca tccccgggcg gctcgtgcca tcgaagccgc
     1141 gcgtcgcgac gggcgagagt ggctcagcgg ctcggaggcc cgcgaggtgg tggctgcggc
     1201 cggcctgccg ttggcacggg ccgtgtcgat cgtcacgcgg gacgactgcg ggaccgcggc
     1261 ggaccgactc acgtttccca tggcgctcaa gcgcgacggt tcggggagcg agcacaagtc
     1321 cgacgaaggg ggcgtgagac tggggatcgc ctcgatccag gaattgcatg cggcgttcga
     1381 tgcgctgggc ggcggcgcaa cgccactcct ggcccaggag atgatttccg gaggccgcga
     1441 gctgatcctg ggaatgaccg tggatcccat agcgggtccg ctgctcatgg cggggaccgg
     1501 tggcaccgag gtggaactct ggcgggacgt ggtgtggcgg attcatccgc tgacggacct
     1561 ggacgccggt gacatgctgc gggcgatgac cggcttcccc aggctttcag gctatcgcgg
     1621 cggcgccccg gtggacattg ccgggatcga gggcgacctg ctccggctgt cgtcgctggt
     1681 gggggaattt ccggagatcg ccgaactgga catcaacccg tatatcgcgc gccccgcggg
     1741 cgccggcaac cggatcgtcg acgtgcgtgt ccggctggcc cctcagattt cttcgggcgg
     1801 ggcttcctga tcgcaggtgc gcagccgctg ctcgaggaac tcgtgcagcc ggtgcgacac
     1861 ctcctgcgga atgtcctcgc accggaactc acccgtcaat tccaccgtga ccttgaagga
     1921 acgaagaaac tggtcgcagt gcgggcagcg tccgaagtgg gtgtccagga gccgctgggt
     1981 ttccggttcg agctcaccgt ccacatagtc ggtgagaagc tggatgacgt cgtcgcagtt
     2041 gaacatgatg gtgcggccgg gtcaggccgg ccctccgagg taatcgccaa gcttgcgccg
     2101 gagcgcgagc cgggcccggt gaagccgtga cttcacggcc gggatcgaca ggttgaggat
     2161 gtcggccact tcctggttcg agagattctg gatgtcgcgc aggaccacca ctgtcttgaa
     2221 atcgtccggc agtgcctgta tggccgactc aagcgcctcg cgcgtctcct tgttgaagag
     2281 ggcgagatcg ggcttctggg cccattccga ggagccgtcg tccgctccgg cctcctcgat
     2341 caccgggagg acgtcgtcca gcgacaccgc cgcgtggcgc ttgcgctggt tcagccgcgc
     2401 gaaggccgcg ttggcggtga tgcggtagag ccagctgctg aacgcggcat gcccgcggaa
     2461 cgtgtggatc ttct
//
LOCUS       DASVOD010000745         2472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_310660, whole genome shotgun sequence.
ACCESSION   DASVOD010000745 DASVOD010000000
VERSION     DASVOD010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310660"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2328)
                     /locus_tag="VF720_16015"
     CDS             complement(<1..2328)
                     /locus_tag="VF720_16015"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX7880918.1"
                     /translation="MMDRHSLDVLEFPRVLAQAVRHAASERGAEWIAALEPTPDAPIA
                     AARLDLTDELRRLITSADGFPPLDTPDVRRALGTLGAAGSVLDVPELRDLARLCLGAR
                     RVRERLAKEAEHYPLAFALSGRLTPAPHFERAVADTFDEHGEILDSASGALRTIRRHL
                     KQQREKITSRLDSYRKGDDAERTITLRADRYVLAVKAGEKGEIGGIVHDRSGSGATVY
                     LEPTALFEENNRLIELLVEERDEIRRILAALSDMVRGLREPLGVNAEVLAEFDAHKAM
                     ARLAESQNAMRPALATDSITLRAFRHPLLGDARNGEAPREVVPLDLELTPDARMLLIT
                     GPNMGGKTVALKGVGLACLMAAVGMHLPAGDGTEVVILADVVADIGDEQSIEQDLSTF
                     ASHLKRWVEAANATALPRLALLDEIGAGTDPAEGAALARAVLERLAAGGGFTVATTHL
                     GALKTFAIDQPGVVNGSMRFDPVHEKPLYRLDQGLPGQSRAIEMARRLGLDSALTDRA
                     LELVGREERSVQALLSELEGTRRELEAEMARAREETARIQEEHARVDDVRRKYEERLK
                     KHAGEYAAVKARAGREAQRHLDKAVDLLKEAETEARGIRQKALIESKEGQKRVENLKE
                     AIKEAREVAPVVRSVAVTAPGRPIDATEVRIGNRFWAEPLAAWVTVVAPPDGTKVRVE
                     RNGLKVELPINALRGGVGAEGAAPAHRTALDELPDDPSGRPKGGFHYAMRDDIKAEVD
                     LRGLTAEEAIDRLDHFLDESMLGAVPKIRVIHGKGT"
BASE COUNT          338 a          860 c          860 g          414 t
ORIGIN      
        1 ggtccccttc ccgtggatga cgcgtatttt cggcacggcg ccgagcatcg attcgtcgag
       61 gaaatggtcg agccggtcga tggcctcctc ggcggtcagg ccccgcaggt cgacttcggc
      121 cttgatgtcg tcgcgcatgg catagtggaa gccccccttc ggccggccgg agggatcgtc
      181 cggaagctca tccaacgcgg tgcgatgggc cggcgcggcc ccttcggccc cgacgccacc
      241 gcgaagggcg ttgatcggca attccacctt gaggccgttc cgttccaccc gcaccttggt
      301 gccatccggc ggcgcgacga ccgtgaccca cgcggcgagg ggctcggccc agaagcgatt
      361 gccgatgcgc acctcggtgg catcgatcgg gcgccccggc gccgtgaccg ccaccgagcg
      421 gaccaccggc gccacctcgc gcgcttcctt gatggcttcc ttcaggtttt cgacccgctt
      481 ctgcccttcc ttcgactcga tgagcgcctt ctgccggatg ccgcgcgcct cggtctcggc
      541 ttccttgagg aggtcgaccg ccttgtcgag atgacgctgg gcctcgcgac cggcccgcgc
      601 cttcacggcg gcatactcgc cggcatgctt cttcaggcgt tcctcgtact tccggcggac
      661 gtcatccacc cgggcatgtt cctcctggat gcgggccgtt tcctcgcggg cacgcgccat
      721 ctcggcttcc agctcgcgcc gggtgccttc gagctcggac aggagcgcct gcacgctccg
      781 ctcctcgcgc ccgaccagct cgagtgcgcg gtcggtcaag gcgctgtcca agcccaggcg
      841 gcgggccatc tcgatggcgc ggctctggcc gggcaatccc tggtcgagtc ggtaaagcgg
      901 cttctcgtgc accgggtcga accgcatcga gccgttcacc acgccgggct ggtcgatggc
      961 gaacgtcttg agggcgccca ggtgcgtggt ggccaccgtg aagccgccgc cggccgccaa
     1021 ccgctcgagc acggcccggg ccagcgccgc gccttcggca ggatcggtgc cggcgccgat
     1081 ttcgtcgagc aacgcgaggc gtggcagcgc ggtggcgttg gccgcctcca cccagcgctt
     1141 caggtgcgag gcaaaggtcg agaggtcctg ttcgatcgac tgttcgtcac cgatgtcggc
     1201 cacgacgtcg gcgaggatca cgacctccgt gccgtctccc gccggcaggt gcatcccgac
     1261 ggcggccatc aggcaggcca ggcccacgcc tttcagcgcc accgtcttgc cacccatgtt
     1321 ggggccggtg atgagaagca tccgcgcgtc gggcgtgagc tcgaggtcga gcggcaccac
     1381 ctcgcgcggc gcctcgccgt tccgcgcgtc tccgaggagc ggatgccgga acgcgcggag
     1441 cgtgatggaa tcggtggcca gtgccggccg catggcgttc tgcgattcgg ccaggcgggc
     1501 catcgccttg tgagcatcga actcggccag cacctcggca ttcacgccga gcggttcgcg
     1561 caagccgcgc accatgtcgg agagcgcggc caggatgcgg cggatctcgt cacgttcctc
     1621 cacgagcagt tcgatcagcc ggttgttctc ctcgaagagc gccgtcggtt ccaggtagac
     1681 ggtggcgccc gagccggagc gatcgtggac gatgccgccg atctccccct tctcgcccgc
     1741 cttgacggcc agcacgtagc ggtcggcccg cagggtgatc gtgcgctcgg cgtcatctcc
     1801 cttgcggtac gaatcgagcc gcgaggtgat cttttcccgc tgctgcttga ggtgccgtcg
     1861 gatggtgcgc agcgcgccgc tggccgagtc gaggatttcg ccatgctcgt cgaacgtgtc
     1921 ggccacggct cgttcgaagt ggggcgccgg cgtgaggcgc ccggacaacg cgaatgccag
     1981 tggatagtgc tcggcctcct tcgccaggcg ttcacgcacg cggcgcgcgc ccaggcagag
     2041 gcgggccagg tcgcgcagct ccggcacgtc gagcaccgat ccggccgcgc cgagcgtgcc
     2101 gagcgcgcgc cggacatcgg gcgtgtcgag cggcgggaag ccgtcggccg aagtaatcaa
     2161 ccggcgcagc tcatcggtca ggtcgaggcg ggcggcggca atcggtgcgt cgggtgtcgg
     2221 ctcgagcgcg gcgatccatt cggcgccccg ctccgaagcg gcatggcgca ccgcctgggc
     2281 caggacccgt ggaaactcga gcacgtcgag ggagtgacga tccatcattc gcgggaagtc
     2341 gttccttccg aaagacgcgg gggctgcagc cgtacttccg gtggtttgga gctgtcgttt
     2401 gtcggggaca tcaggaatac gccgcgaacc gccagccgat tgacggctgg cggtcgggct
     2461 gaatatcggg at
//
LOCUS       DASVOD010000746         2451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_725850, whole genome shotgun sequence.
ACCESSION   DASVOD010000746 DASVOD010000000
VERSION     DASVOD010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725850"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF720_16020"
     CDS             <1..310
                     /locus_tag="VF720_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880919.1"
                     /translation="VEWRLVRPAELTLVDGIESWSGTLERGETRSFEVALTVPDDVRR
                     EVTARAWLPERPRATSAASAAIDLGVLDGLRSTGTSVVADGETYLQYQGLVTPRGEEK
                     "
     gene            307..>2451
                     /locus_tag="VF720_16025"
     CDS             307..>2451
                     /locus_tag="VF720_16025"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7880920.1"
                     /translation="MRGRRALATAARTLSLAATIFVSAGVVSPALANWTATGTFRYRD
                     RPQDLNGFTGATQDRAIRSARVEIVRSPSTVLATGVTDAGGNFNIPVTDAMTATVFAR
                     VISQDNVAVLGIRVQSTTAGSSLYAVTSASVPSHNPNTNTDFGIITALEGAGAEVFNI
                     FEIMLDGSDYVAALTGAYPAQPCTTVWSTSSTTGTFFSGSTIRLLAEEGWDDNVIGHE
                     HGHFIDLNFSATDSPGGPHFLGDNNQDMRLSYGEGTATYHMSAARRLAGKSDPPSYVD
                     CTAGAGAGNLNFSYEVEGPNISALGAASEVTVTAALWDIVDDAATDDQYPGDDDPLSR
                     PIADVWDDMVNYIPTAANISLEDFWDGWFAPGVNNGFLAEMQTTFGGHLCEFYGDPFE
                     GDNTAGTASTIVTDGVPQHHCFYGSGDVDFVRFNSPSPGTGFIVETSNLLGDPNTTLS
                     IIDQNGTTVLATNDDRSAFDQSSLLQWSAPAAGNYYARLMHSPDLGVYGSYNLRVIKG
                     SQSSITWTDVANAAGLASTSPGRGGAWGDYDNDGDVDVFVTAASGSTFQLFRNNGAGV
                     FTDVAAAAGVTGGAVSTGEQAAWGDYDNDGDLDLAVAVIGTNLLYRNNGNSTFTSVGA
                     AAGVNHTGAGSLGLSWCDYDGDGFIDLFVGTIDANDKLYRNNGNGTFTDMAAAAGVGG
                     AGEDTFGGIWADYDLDNDADLFVHYDGDPNRLF"
BASE COUNT          424 a          890 c          781 g          356 t
ORIGIN      
        1 ggtggagtgg cggttggtac ggccggccga gctcacgctg gtcgacggga tcgaaagctg
       61 gagcggcacg ctggaacggg gggagacgcg ctcgttcgaa gtcgcgctca ccgtgccgga
      121 cgacgtccgg cgcgaggtga cggcacgggc ctggctcccc gagcggccac gcgccaccag
      181 cgcagcctcg gcggccatcg acctgggcgt gctcgacggc ctccggtcca cgggcaccag
      241 cgtggtggcc gacggcgaaa cgtatctcca gtaccagggc ctcgtgaccc cgcgcgggga
      301 ggaaaagtga gggggcgccg cgcactcgcg acggccgcgc ggacactctc tctcgcggca
      361 acgattttcg tatcggcggg agtcgtttcc ccggcactgg ccaactggac cgccaccggc
      421 accttccgct atcgcgaccg gcctcaggac ttgaacggct tcaccggcgc cacgcaggat
      481 cgcgccatcc ggagcgcgcg ggtggagatc gtgcgcagcc catccaccgt gctggccacc
      541 ggcgtcaccg acgccggcgg caacttcaac attccggtca ccgatgccat gacggccacg
      601 gtcttcgcgc gcgtcatcag ccaggacaac gtggcggtgc ttggtatccg cgtccagtcc
      661 accacggcgg gatcctcgct ctacgcggtc acgtcggcca gcgtgccgag ccacaatccg
      721 aacacgaaca ccgacttcgg catcatcacg gcgctggaag gggcgggggc cgaagtcttc
      781 aacatcttcg agatcatgct cgacggcagc gactacgtgg cggcgctcac cggcgcctac
      841 ccggcccagc cctgcacgac cgtctggagc acctcctcga ccaccggcac cttcttctcg
      901 ggttccacca tccgactcct cgccgaagag gggtgggacg acaacgtgat cggccacgag
      961 cacggccact tcatcgacct gaacttctcg gccaccgata gccccggcgg gccgcacttc
     1021 ctgggagaca acaaccagga catgcgcctc tcgtacggcg aaggaacggc cacctaccac
     1081 atgtcggccg cgcggcggct ggcggggaag agcgaccccc cgtcgtatgt ggactgcacg
     1141 gcgggcgcgg gagccggcaa cctcaacttc tcgtacgaag tggaagggcc caacatctcg
     1201 gcgctcggcg cggccagtga agtcacggtg acggccgccc tctgggacat cgtggatgac
     1261 gccgccaccg acgaccagta tccgggcgat gacgatccgc tctcgcgtcc catcgcggac
     1321 gtgtgggacg acatggtcaa ctacatcccg acggccgcca acatctcgct cgaggacttc
     1381 tgggacggct ggttcgcccc gggggtcaac aacggcttcc tggccgagat gcagaccact
     1441 ttcggcggcc atctctgcga gttctacggc gatccgttcg aaggggacaa caccgccggc
     1501 accgccagca ccatcgtcac cgacggcgtg ccgcagcacc actgcttcta cggcagcggc
     1561 gatgtcgact tcgtccgctt caactcgccg tcgcccggca ccggcttcat cgtggaaacc
     1621 agcaacctcc tgggcgatcc gaacaccacg ctcagcatca tcgaccagaa cggcacgacc
     1681 gtcctggcca ccaacgacga ccggagcgcc ttcgaccagt cgtcgctcct ccagtggtca
     1741 gctcccgcgg ccggcaatta ctacgcccgc ctgatgcatt cgcccgacct cggcgtgtac
     1801 ggctcctaca acctgcgcgt catcaagggg agccagtcgt ccatcacctg gaccgacgtg
     1861 gccaacgcgg ccggcctggc ctccacctcg ccggggcgcg gcggcgcctg gggcgattac
     1921 gacaacgacg gcgacgtgga cgtcttcgtg accgccgcct cgggaagcac cttccagctc
     1981 ttccggaaca acggcgccgg cgtcttcacc gacgtggcgg ccgccgccgg cgtgacgggc
     2041 ggtgccgtgt ccaccggcga gcaggccgcc tggggcgatt acgacaacga cggcgacctc
     2101 gacctggccg tggccgtgat cggcaccaac cttctttacc ggaacaacgg caactcaacg
     2161 ttcaccagcg tgggcgcggc cgcgggcgtg aaccacaccg gcgccggcag cctcggcctc
     2221 tcctggtgcg attacgacgg agacggcttc atcgacctct tcgtcggcac gatcgacgcg
     2281 aacgacaagc tctaccggaa caacggcaac ggcaccttca ccgacatggc cgccgcggcc
     2341 ggcgtgggcg gggcggggga ggatacgttc ggcggcatct gggccgacta cgacctggat
     2401 aacgacgccg acctcttcgt gcactacgac ggcgatccga accgtctctt c
//
LOCUS       DASVOD010000747         3151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_778967, whole genome shotgun sequence.
ACCESSION   DASVOD010000747 DASVOD010000000
VERSION     DASVOD010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778967"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1915)
                     /gene="uvrC"
                     /locus_tag="VF720_16030"
     CDS             complement(89..1915)
                     /gene="uvrC"
                     /locus_tag="VF720_16030"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX7880921.1"
                     /translation="MNNELRRKLDTIPRRPGVYLMKDKGGRIIYVGKAKRLDLRLRSH
                     FSGALDHPKQRAMIGKTVDLDVIATDSDVDALILEMTLIKEKRPVYNINLKDDKRFPF
                     VKVTMQEAFPRIELTRRVKEDGARYFGPYTDVGALRQSLRMLRTVFPVRSCPGDRPGR
                     GPGRRECLDFYIHRCLAPCIKPEVADEYRSAAEAFCLFLSGGAEAVVARLKGEMEAHA
                     ARLEFEAASRRRDQIRLIERIQRRQRMVDVQKRDTDLCAFTRSGDLAWGVVLQIRDGR
                     VIGKERRTLKGVAHTDDAEVMAAFLLQHYDRADMLPAQVAVAVLPVDDELITDWMARR
                     TGKAVKLVVPKKGPLAQLVRLALANARLDMEEARGLRKGKELPTGLYELQRLLDLEGP
                     PVRIECFDISNIQGTHPVASMVMMVAGRPSRGDYRRYRMTTPGPDDFAMMREAVGRRA
                     RRVAAGEFEPPDLVLIDGGAGQVSAAREALAAEGLGHLPVIGLAKRQEEIVVPGQPQP
                     IRLPRTHEALKLLIQLRDEAHRFAVSYHRNRRGKAALQSRLEGIPGIGAERRLALLQH
                     FGSVEAMRQATADDLARVPGLGPATVRRLLEALQADDGGVAA"
     gene            complement(2118..>3151)
                     /locus_tag="VF720_16035"
     CDS             complement(2118..>3151)
                     /locus_tag="VF720_16035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7880922.1"
                     /translation="IMLEQMEEAVFLCGADGVVGLITPAAEKLMAGRPCGGLPMVEAF
                     PDELKDDLATILAGITSPEGRHQTREFRIEGRIHLASFTAIRDAGGHLIGMMSLWKDI
                     TDLKTIESQLMMSSKMSAVGQLASGVAHEFNNLIAAIYGYAQFMKDNRDEKLFDKGIR
                     IILASSERARDLTRSLLTFSRASEGLVEPLGPNELMDDVLLLVEQQLIKEGIRVERQY
                     GRLPHAFAERGRLQEVFLNLVSNARHAMPDGGILTLASRSEGSEVVIEVTDTGVGIPP
                     EHLSRIFEPFFTTKGPLNATRVPGTGLGLFTVYNTVQAHGGRTEVTSAPGQGTTVRLI
                     FPSGLRAAA"
BASE COUNT          490 a         1076 c         1061 g          524 t
ORIGIN      
        1 agacgatcga cgggcgtctc ccccatcgtt cggaccccgt gcgcggcacc cgtcgccggc
       61 acacgcggtt gtgcccgtgt ccgtggcgtc acgccgcaac cccgccgtcg tcggcctgga
      121 gcgcctcgag aagccggcgc accgtggcgg gcccaagccc gggaacgcgc gccaggtcgt
      181 cggcggtggc ctggcgcatg gcctccaccg atccgaagtg ctggaggagc gccagccgcc
      241 gctcggcccc gatgccggga attccctcga gtcgtgactg gagcgccgcc ttgccgcgcc
      301 ggttccggtg gtacgacacg gcaaagcggt gggcctcatc gcggagctgg atgaggagct
      361 tcagcgcctc atgcgtgcgg ggcaggcgga tcggctgcgg ctgccccggc accacgattt
      421 cttcctgtcg cttggccagc ccgatcaccg gcaggtggcc aagcccttcg gccgccagcg
      481 cctcgcgggc cgccgaaacc tggccggcgc ccccgtcgat caacacgagg tcgggtggtt
      541 cgaactcacc cgccgctacg cgccgagccc gccgtccgac cgcctcgcgc atcatcgcaa
      601 agtcgtcggg cccgggagtc gtcatccgat accggcggta atcgccgcgt gacgggcgcc
      661 cggccaccat catcaccatc gaggccaccg ggtgcgtccc ctggatgttg gagatgtcga
      721 agcactcgat gcggaccggc ggcccttcga ggtcgagcag tctctgcagc tcgtacaggc
      781 cggtcggcag ttccttcccc ttgcgcaatc cgcgcgcctc ttccatgtcg agccgggcat
      841 tggccagggc caggcggacg agctgcgcca gcggtccctt cttcggcacg accagcttga
      901 cagccttccc cgtgcgacgg gccatccagt cggtgatcag ttcgtcatcg accggcagga
      961 ccgccacggc cacctgggcc gggagcatgt cggcccggtc gtagtgctgc agcaggaagg
     1021 cggccatgac ctcggcgtca tcggtgtggg ccacgccctt cagcgtccga cgctccttgc
     1081 cgatcacgcg gccgtcgcgg atctggagca ccacgcccca cgccaggtcg cccgagcggg
     1141 tgaaggcgca gaggtcggtg tcgcgcttct gcacgtccac catccgctgc cggcgctgga
     1201 tgcgttcgat caggcggatc tggtcgcgcc ggcgggaggc cgcctcgaac tcgaggcgcg
     1261 cggcgtgcgc ctccatctcc cccttgagcc gggccaccac ggcctcggcg ccgcccgaca
     1321 ggaagaggca gaacgcctcg gcggcgctcc ggtactcgtc ggccacttcg ggcttgatgc
     1381 aaggcgccag gcatcgatgg atgtagaagt cgaggcactc tcgccgtccc ggaccgcgcc
     1441 ccggcctgtc acccggacac gagcggaccg gaaagaccgt gcgcagcatc cggagcgact
     1501 gccgcagcgc acccacatcg gtgtacggcc cgaagtagcg ggcgccatct tccttcacgc
     1561 gccgcgtcag ctcgatgcgc gggaacgcct cctgcatcgt caccttcacg aagggaaagc
     1621 gtttgtcgtc cttgaggttg atgttgtaga cggggcgctt ttccttgatc agcgtcatct
     1681 cgaggatcag cgcgtcgaca tccgagtcgg tggcgatcac gtcgagatcg acggtcttcc
     1741 cgatcatggc ccgctgcttg ggatggtcca gggccccgga aaagtgggaa cgcaggcgca
     1801 ggtcgagcct cttggccttg ccgacgtaga tgatccggcc gcccttgtcc ttcatgaggt
     1861 aaacgccggg ccgccgggga atcgtgtcca gcttccgacg aagctcgttg ttcatgctcg
     1921 agaggctccc ccccgggaca ccccagagga cccggtgtgg cggccctaag tggctacaat
     1981 gtaagcagtt aatagctttc cttcacgctc cacacacggg tgcagagttt gacggaaaac
     2041 gctactcccc cgggcgggac aaggcaagcc aaatcgccgg gaggcgcgcg gggaccgggg
     2101 gcggatttcg ggccggatca ggcggcggcg cggaggccgg acgggaagat cagtcggacg
     2161 gtggttccct ggccgggggc gcttgtcacc tcggtgcgtc cgccatgggc ctggaccgtg
     2221 ttgtacacgg tgaagaggcc gaggccggtg cccggcacgc gcgtggcgtt cagcggtccc
     2281 ttggtggtga agaacggctc gaagatgcgc gagaggtgct cggggggaat gcccacgccg
     2341 gtatcggtaa cctcgatgac cacttcgctc ccctccgacc ggctggccag ggtcaggatg
     2401 cccccatcgg gcatcgcgtg gcgcgcgttg gaaacgagat tcaggaagac ctcctgcagc
     2461 cgcccccgtt cggcgaaggc atggggaagg cggccgtact gccgctcgac ccggatcccc
     2521 tccttgatca gctgctgttc gacgaggagc aggacgtcgt ccatcagctc gttcggaccg
     2581 agcggttcca caagtccctc gctggctctc gaaaaggtga ggaggcttcg cgtgagatcc
     2641 cgggcgcgct cgctcgaggc caggatgatc cggataccct tgtcgaacag cttctcgtcg
     2701 cgattgtcct tcatgaactg ggcgtagcca tagatggccg cgatgaggtt gttgaattcg
     2761 tgggcaacgc cggaagcgag ttgtcccacc gccgacatct tgctcgacat catgagctgc
     2821 gattcgatcg tcttcaggtc ggtgatgtcc ttccagagcg acatcatccc gatcaggtgg
     2881 ccccccgcat cgcgaatggc cgtgaaactg gccaggtgga tgcgcccctc gatgcggaat
     2941 tcgcgggtct ggtgccgccc ttccggcgag gtgatgccgg ccaggatcgt ggccaggtcg
     3001 tccttcaatt cgtccgggaa cgcctccacc atcggcaggc cgccgcaggg gcggccggcc
     3061 atcagtttct cggcggcggg agtgatgagc cccaccacgc cgtcggcgcc gcacaggaag
     3121 accgcctcct ccatttgctc gagcatgatc g
//
LOCUS       DASVOD010000748         3786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_990611, whole genome shotgun sequence.
ACCESSION   DASVOD010000748 DASVOD010000000
VERSION     DASVOD010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990611"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VF720_16040"
     CDS             <1..937
                     /locus_tag="VF720_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7880923.1"
                     /translation="IPFTISCGKCFFCSQELYAACETTNPQTSATEGRGAIVNKKGIR
                     SGAALFGYSHLYGGVPGGQAERVRVPQANVGPFRVPNELSDDQVLFLSDILPTGWMAA
                     VNADIRKGSTVAVLGAGPVGLLAVASARVLGASTIFLVDHHAYRLEFGAATYDAIPVN
                     FDTVDDAAEEIIRRTDHRGVDAVIDAVGFEAKGSTTETVMTNLKLEGSSGAALRMAIA
                     MVRRGGVVSVPGVYAGWIHAFLFGDAFEKGITLRGGQTHVKKFLPELLEHIRKGRLHP
                     DAIISHRLPLSEAAEGYRLFNDKDDNCRKVVLKAD"
     gene            955..1857
                     /locus_tag="VF720_16045"
     CDS             955..1857
                     /locus_tag="VF720_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX7880924.1"
                     /translation="MDRNSLAGRVIVITGASSGFGKGTAVAFAREGARLALAARRAEL
                     LEERVTACMEAGADAFALAIDVSDPSAVQDLCRATLDRYGRIDVWINNAGVGAIGRFE
                     RIPIEDHAQVVQTNLLGTIYGSYHAYRQFIEQANGILINVASELGKHTVPYFSSYTAA
                     KHGVVGLGDSLRQELAMAHHGDIHVCTVMPTAHDTPFFDHVANYTGHEIQAPEPLHDP
                     NDVIDTLVALARNPKDETIVGGDGIVKILLKKVVPPMSEKGAAKQMHKTQFEDSPPAG
                     DTSGAVHAPIGAGTEVSAGRRKKP"
     gene            complement(1880..2743)
                     /locus_tag="VF720_16050"
     CDS             complement(1880..2743)
                     /locus_tag="VF720_16050"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX7880925.1"
                     /translation="MISGLPGGPTGGAQPPVRCFSIDVEDWYHFKIDAPETWNSHETR
                     VHVGTDLLLEMLERRGVRATCFILAHVCETAPDLVRRIVAAGHEIASHGYNHQLIYRQ
                     TERQFDEDLKRSIGLIGDLAGKAPTGFRASSFSVSPKTPWFWDVLVRNGITWDSSVFP
                     VKNPFYGGVSALPYPHRMPQHDLIEIPIAPAPVPGLGVPFSGGFYFRLHPMPFLRWGA
                     RALARQGRPVIYYVHPWEYDLAQPRMELPPHWRFFRYHRLAAMRPVTEAVLADGPFVT
                     MGELAAQVPAA"
     gene            complement(2740..3102)
                     /locus_tag="VF720_16055"
     CDS             complement(2740..3102)
                     /locus_tag="VF720_16055"
                     /inference="COORDINATES: protein motif:HMM:NF021575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEX7880926.1"
                     /translation="MLSFRAQMAAALLALGMFLLCLDLVRRRKLKEEYSLLWIGVTAA
                     MTIIAFWKGLLFWIAAQLGILNANTVIFLSGLGFLIMVAVHYSIRLSELTDRNKDLAQ
                     SVAILSSEIEELRKKPRS"
     gene            complement(3105..>3786)
                     /locus_tag="VF720_16060"
     CDS             complement(3105..>3786)
                     /locus_tag="VF720_16060"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX7880927.1"
                     /translation="SIGDLLGEIAATGRGYHTLVVDDGSADATAAVASAHSPVVRLTQ
                     NLGIGGAVQTGFKYAEDHGYDAVIQVDGDGQHDPAEIGLIVGPVARGEADVVIGSRFL
                     RKTAYKAPFTRRVGMVLFASVARWAMKQKITDTTSGFRALNREAFQYFARHYPTDFPD
                     AESLVLLKRAGFRLSEVSVNMKPRKRGTSSTTTLRSLYYPFKMILAVFIVMLREPPAR
                     PKGPGEVR"
BASE COUNT          677 a         1236 c         1302 g          571 t
ORIGIN      
        1 catcccgttc accatctcct gcggcaagtg cttcttctgc agccaggaac tctacgccgc
       61 ctgcgagacc accaatcccc agacgagcgc caccgagggg cggggcgcca tcgtcaacaa
      121 gaaagggatc cgctcgggcg ccgcgctgtt cggctattcg cacctttacg gcggggttcc
      181 gggaggacag gccgagcggg tgcgcgttcc ccaggccaac gtgggaccct tccgggttcc
      241 gaacgagctg tcggacgacc aggtgctctt cctgtccgac atcctcccca ccggctggat
      301 ggccgccgtc aacgccgaca tccgcaaggg ttcgaccgtg gccgtgcttg gagcagggcc
      361 ggtggggctc ctggcagtgg ccagcgcgcg ggtgctcggg gccagcacga tttttctcgt
      421 ggaccatcac gcctaccggc tggagttcgg ggccgccact tacgatgcca tccccgtcaa
      481 tttcgacacg gtggacgacg ccgccgagga gatcatccgg cgcaccgatc atcggggcgt
      541 ggacgcggtc atcgacgcgg tgggattcga ggccaagggg tcgaccaccg aaaccgtcat
      601 gacgaacctg aagctcgagg gatcgagcgg cgcggccctc cgcatggcca tcgcgatggt
      661 ccgccgcggc ggtgtggtca gcgtgccggg cgtctatgcg gggtggatcc acgccttcct
      721 tttcggcgac gccttcgaaa aggggatcac cctgcggggc ggccagaccc acgtgaagaa
      781 gttcctgccc gagcttctcg agcacatccg gaaagggcgt ctccaccccg acgccatcat
      841 cagccatcgg cttccgctgt cggaggccgc cgaggggtat cgcctcttca acgacaagga
      901 cgacaactgc cggaaggtcg tgctgaaagc cgactgaccg gggaggagcg catcatggac
      961 aggaattcac tggccggccg cgtgatcgtg atcaccggtg cctccagcgg cttcgggaag
     1021 ggaacggccg tggcgtttgc ccgggaagga gcccggctgg cgctggccgc ccggcgggcc
     1081 gagctcctgg aggagagggt aacggcctgc atggaggccg gagcggacgc gtttgccctg
     1141 gccatcgacg tgagcgaccc cagcgccgtg caggatctct gccgggccac gctcgatcgg
     1201 tacgggcgca tcgatgtctg gatcaacaat gccggtgtcg gcgccatcgg gcgcttcgaa
     1261 cggatcccca tcgaagacca cgcccaggtg gtgcagacca acctgctcgg cacgatctac
     1321 ggcagctacc acgcctacag gcagttcatc gagcaggcca acggcatcct catcaacgtg
     1381 gcctccgagc tggggaagca cacggtcccc tacttctcgt cgtacaccgc ggccaagcat
     1441 ggcgtggtgg gcctgggcga ttccctccgg caggagctgg ctatggccca ccacggcgac
     1501 attcacgtct gcacggtgat gccgaccgcc cacgacacgc ccttcttcga ccacgtggcc
     1561 aactacaccg gccacgagat ccaggcgccc gaaccgctgc acgatccgaa cgacgtgatc
     1621 gacacgctgg tggcgctggc ccggaacccg aaggacgaaa cgatcgtggg tggcgacggc
     1681 atcgtgaaga tcctgctcaa gaaagtggtc ccgccgatgt cggaaaaggg cgccgcgaaa
     1741 cagatgcaca agacgcagtt cgaggattcg ccaccggccg gggacacgag cggcgccgtg
     1801 cacgcgccga tcggtgccgg aacggaggtc agcgcagggc ggcggaagaa gccgtagcca
     1861 agcactcggg gtcccgcgat caggcggcgg gcacctgcgc ggccagctcc cccatcgtga
     1921 cgaacggccc gtcggcgagc accgcctcgg tgacgggccg catggccgcc agccggtgat
     1981 aacggaagaa gcgccagtgc ggcggcagct ccatgcgggg ttgcgcgagg tcgtactccc
     2041 acggatgcac gtagtagatg accggtcgcc cctgtcgggc aagcgcccgc gcgccccaac
     2101 gcaggaaggg catcgggtgt aagcggaagt agaagccccc cgagaacggc acgccgaggc
     2161 cgggaaccgg ggccggcgcg atcgggatct cgatgaggtc gtgctgcggc atccgatgcg
     2221 ggtacggcag tgcgctcacg ccgccgtaaa acggattctt caccgggaag acgctcgagt
     2281 cccaggtgat gccgttccgg accagcacgt cccagaacca gggagtcttc ggcgacacgc
     2341 tgaacgagct ggcccggaag ccggtgggcg ccttccctgc caggtcgccg atcaggccga
     2401 tcgagcgctt caggtcctcg tcgaactgcc tctccgtctg gcgatagatg agctggtggt
     2461 tgtagccatg gctggcgatc tcgtggccgg ccgccacgat gcggcgcacg aggtccgggg
     2521 cggtctcgca tacgtgggcc aggatgaagc aggtggcccg gacgccgcgg cgttcgagca
     2581 tctcgaggag gagatcggtt ccgacgtgca cgcgcgtctc gtgggaattc cacgtctcgg
     2641 gcgcgtcgat cttgaagtgg taccagtcct cgacgtcgat cgagaaacag cggacggggg
     2701 gctgggcgcc gcccgtcggg ccgcccggca agccggaaat cacgaccgcg gcttcttcct
     2761 cagctcctcg atctcgctcg agaggatggc caccgactgc gccaggtcct tgttgcggtc
     2821 ggtgagctcg gagaggcgga tcgagtagtg aaccgccacc atgatcagga atccgaggcc
     2881 cgagaggaag atgacggtgt tggcattgag gatgccgagc tgcgccgcga tccagaaaag
     2941 gagtcccttc cagaaggcga taatggtcat ggccgccgtg acgccgatcc agaggagcga
     3001 atactcctcc ttgagcttgc ggcggcggac caggtcgagg cacaagagga acatgccgag
     3061 cgccaggagc gcggccgcca tctgggcacg gaacgagagc atggctagcg aacctccccg
     3121 gggcctttgg gccgggccgg cggttcgcgc agcatcacga tgaagacggc caggatcatc
     3181 ttgaagggat agtagagcga gcggagcgtg gtcgtcgacg acgtgccgcg cttgcgcggc
     3241 ttcatgttga ccgacacctc cgacagccgg aatccggcgc gcttcaagag cacgaggctt
     3301 tcggcatcag gaaagtcggt cgggtaatgc cgggcgaaat actggaacgc ctcgcggttc
     3361 agcgcccgga atcccgaagt ggtgtcggtg atcttctgct tcatggccca gcgggccacg
     3421 ctggcaaaga gcaccatccc cacccgacgc gtgaacggcg ccttgtaggc cgtcttcctg
     3481 aggaagcggc tgccgatgac gacatcggcc tcgccgcgcg ccaccgggcc cacgatcagg
     3541 ccgatctcgg ccgggtcgtg ctgcccgtcg ccgtccacct ggatgacggc gtcgtaaccg
     3601 tgatcctcgg catacttgaa gccggtctgc accgcgccgc cgatgccgag attttgcgtg
     3661 agacgcacga ccggcgagtg cgcgctcgcg accgcggcgg tggcgtcggc cgagccgtcg
     3721 tccaccacca gcgtgtggta gccgcgtccg gtcgcagcga tctcgccgag gagatcgccg
     3781 atcgag
//
LOCUS       DASVOD010000749         1959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_884402, whole genome shotgun sequence.
ACCESSION   DASVOD010000749 DASVOD010000000
VERSION     DASVOD010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884402"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VF720_16065"
     CDS             <1..1071
                     /locus_tag="VF720_16065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX7880928.1"
                     /translation="DTREERARLRQLLDENRPPEMGLIVRTAGEGKSRQDFRNDVRYL
                     LDLWRKIEERANRAEPPALLHREMSLTTGLIRDILTEDVSEVVIDNPTVLAEVQEYVR
                     SLSPELVDRVKPYQGNLAVFDSYGVEGDIERLLERKVWLKKGGHLIIDQTEALIAIDV
                     NTGRFVGKKNQEETILKTNLEAAREVARQLRLRDVGGIIIIDFIDMEQESNKKQVLDQ
                     LRQGLKRDRSRTKTTGISDLGIVEMTRERERQSLLDILSVECAHCGGLGKVLSLNSLG
                     LKVERLLRRIGAAGKERVIELRVSPELADHLFHDQGARIERLEKQFGYRIDIRDDPRL
                     GRDEVRVIYPRTGEDMTEKFKV"
     gene            1298..>1959
                     /locus_tag="VF720_16070"
     CDS             1298..>1959
                     /locus_tag="VF720_16070"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HEX7880929.1"
                     /translation="MRYLPSARLGLRPACSTNPRLLIPILAWLMLGISSPAHGESVVL
                     APVADNTLIQEGANDRSNGAGEYLFVGKTDTGNIRRLLVRFDVAGSIPPGSTITGATL
                     TFFCSRAKVNTADAFELHRALVSWGEATANSGGQEGSGTAAVTGDVTWLYRFYPTLAW
                     GSPGGDMSGTVSASTAVGANGQSYSWSAPGMTVDLQSWLDAPAGNFGWVLLGGESEAS
                     RSA"
BASE COUNT          352 a          667 c          642 g          298 t
ORIGIN      
        1 gacacgcggg aggaacgggc ccggctccgg cagctcctcg acgaaaaccg tccgccggaa
       61 atggggctca tcgtccgcac cgccggggag gggaagtcgc gccaggattt ccggaacgac
      121 gtccgctacc tgctcgacct ctggcgcaag atcgaggagc gcgccaatcg cgccgagccg
      181 ccggcgctgc tccaccggga gatgtcgctc accaccggcc tcatccgcga catcctcacc
      241 gaggatgtca gcgaggtggt catcgataac cccaccgtgc tggccgaggt gcaggagtac
      301 gttcgcagcc tctcgcccga gctggtcgac cgcgtgaagc cgtaccaggg gaacctggcc
      361 gtcttcgaca gctacggcgt ggaaggggac atcgagcgcc tgctcgaacg gaaggtgtgg
      421 ctcaagaaag gtggccacct catcatcgac cagaccgagg cgctgatcgc catcgacgtg
      481 aacaccggcc ggttcgtcgg caagaagaac caggaagaga ccattctgaa gacgaacctg
      541 gaagccgcgc gcgaagtggc ccgccagctc cgcctgcgcg acgtcggcgg catcatcatc
      601 atcgacttca tcgacatgga gcaggagtcc aacaagaagc aggttctcga ccagctccgc
      661 cagggtctca agcgcgaccg ctcccgcacc aagaccaccg gcatctccga cctcggcatc
      721 gtggagatga cccgcgagcg cgaacgacag agcctcctcg acatcctgtc ggtggagtgc
      781 gcgcattgcg gcgggctcgg caaggtgctt tcgctcaact cgctcggcct caaggtggag
      841 cgcctgctcc gccggatcgg cgcggcgggg aaggagcggg tcatcgagct gcgggtgagc
      901 cccgaactgg ccgaccacct cttccatgac cagggggcgc ggatcgaacg actggagaag
      961 cagttcggct accgcatcga catccgcgac gatccccgtc tcggccgcga cgaagtgcgg
     1021 gtgatctacc cccgcacggg cgaggacatg accgagaagt tcaaggtatg acgggaccgg
     1081 cggagcccga cccggatttt tctcccgcga ggccatctcc gccgaagctc gattcacgat
     1141 aaatgtcacc ctggaggagc ttccctcctt gagcgggaat accctcggac tagactccgg
     1201 ttgaacgggg cgcgcgccgc gtcggcgccg ccgccgtgga ccgaaggccc cgatcgaata
     1261 tcgtcgccat cgtggacccc cggtccggga ggtgacgatg cggtacctgc catcggcccg
     1321 acttgggctc cgccccgcct gttccacgaa cccccgcctg ctgatcccca tcctcgcctg
     1381 gctgatgctc ggcatctcga gcccggcgca tggtgagtcg gtcgtcctcg cccccgtagc
     1441 cgacaacacg ctgatacagg aaggggccaa tgaccggagc aatggcgccg gcgaatatct
     1501 tttcgtcggc aagaccgaca ccggcaacat ccggcgactc ctcgtccggt tcgatgtggc
     1561 cggctccatt ccgccgggtt ccaccatcac cggcgcgacg ctgacgttct tctgcagtcg
     1621 tgcgaaggtc aacaccgcgg acgctttcga gctgcaccgc gcgctggtgt cgtggggcga
     1681 agcgacggcg aattccgggg gccaggaggg gagcgggacc gcggcagtga cgggggacgt
     1741 cacctggctc taccgcttct atccgaccct ggcctggggc agtcccggtg gagacatgtc
     1801 cggaaccgtg agcgcctcga ccgcggtcgg cgcgaacggc cagtcctaca gctggtcggc
     1861 tcccggaatg accgtcgacc ttcagtcgtg gctcgacgct cccgccggaa acttcggctg
     1921 ggtgttgctg gggggcgagt cggaggccag ccgatccgc
//
LOCUS       DASVOD010000750        10405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_260263, whole genome shotgun sequence.
ACCESSION   DASVOD010000750 DASVOD010000000
VERSION     DASVOD010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260263"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..596)
                     /locus_tag="VF720_16075"
     CDS             complement(48..596)
                     /locus_tag="VF720_16075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX7880930.1"
                     /translation="MVEESGVPLTFWKEAYEEHGGAVLAFLTSRVRKRDDAEDLLQET
                     FIRAIRGGSALRDDALVKPYLMTIAYRVMIDRSRKKRHLLLLDLVNDESAGNWEAVDA
                     SPSPEEEAHLSGLTNRVGEVLETLPAALAQAFRLAVLEQRSYKEIGDLHGWPMSRVRM
                     NVYRARQKVIAALGPVLSESDS"
     gene            845..1462
                     /locus_tag="VF720_16080"
     CDS             845..1462
                     /locus_tag="VF720_16080"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX7880931.1"
                     /translation="MSTAASAQAPEPGRLLRIRVSLNGRPLKSQVFDKDVVLVGRDPE
                     CDVVLDNTGVSRHHLKFELGPTGFYSVTDLGSANGTLFNEQPLKKDFIYSGGDVLQIG
                     KFTLWVTRESDRRADEPDRRPTPEAEARTTVLSATELLKIEQEARQRRMASITALPTA
                     APVAEKPQVRRESKASPRMTHAAALLVGLLAGMLVQRLLSLVIGI"
     gene            1500..3959
                     /locus_tag="VF720_16085"
     CDS             1500..3959
                     /locus_tag="VF720_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX7880932.1"
                     /translation="MKLVDHLQAGRHIAALRSHPHPSDPRRIRARRQLIALGPGAVPP
                     LLGALKSLDVRAAAGEILFELLDDRTLPFFLEALANPEPATARVVLDILTRNQGYDPT
                     QLLDLFADSSVPKSRVEALLVAQMPRIQPRLLVTLIPEMSREARALTFRLLDRHADAA
                     AVLESIRLADDPDWWIRFQMARLLARVAHPDGATVLIRLLADENRGVRLEATRSLGRL
                     RDPRAVPALCRTLRDADLRVQTGAIDALIGIGHASAVPHLVEVLMDESETARRAAVEV
                     LNEVATADAIKDLVMALRDADWWVRSRAADALGTLGGPPVVDAVLAILDDPDEQVRRY
                     AVEILNVATDTRAVEPLIRALDDTDWWVRERAIDALARTRDSRAVEPLAAQLFRDADA
                     AALCVSALRRIGHPSALPIFERLIDSESAELRRGAIEGLLELFTGELSADERRRVVAL
                     LADVGVDPTRRGSPIQESRPTVARTPGNSSGERPASATGEMPAAPVTPSNAPVTPANA
                     TPRIAPDSPSLPAAPRVFRDVTQLPPGEVLVDRYRVVRKVGGGGFGTVYLVEDVLIRD
                     ELVLKVLNPQLTHDATMTRRFLQEMRVSRRITHPNVIRTHELVDLGGWHAIAMEYFPS
                     RDLGDVLKSEGRMSPERALAIGIQVCEALAAAHERGTIHRDVKPGNILIDENDQVKVV
                     DFGLAAIGQRSGSRLTMSGTLLGTPEYMAPEQITDGSADARSDIYSLGAVLYEMLSGV
                     SPFAGENTAHVLFQHIEGDIRPISEAAPGLPEGLDAVVMSMLARAQADRPDTANGLAE
                     RLRAIRAVQGGADGAGRVAAA"
     gene            3956..5104
                     /locus_tag="VF720_16090"
     CDS             3956..5104
                     /locus_tag="VF720_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018233609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7880933.1"
                     /translation="MTSSFRSREAGRRIDAFLDLGREHGASDIHLSAGLPPLVRTDGE
                     LLPAPHRPLADDEIAALIDEILNEDQQRIFAERGSTDLSYQSARGGRYRINICRHLHG
                     IAAICRVIPSDVPRLAHLGLPSPVARLTAFRSGLVLVTGPTGTGKSTTLAALVNEINQ
                     NKSLNILTLEDPVEFLHTSARSQVVQREIGTDVPSFEAGLRAALREDPDVILVGELRD
                     PTTISLALEAAETGHFVLGTLHTRGAHQTIDRIVDAYPAEAQAQVRHTLADNLRCVLS
                     QELVKAADGRGRRVAMEIMVGTPAVAQLIRDGKTFQLQTAIATGRRVGMQLMDLALIA
                     LVRAGEIDPNEAFLRAADKREFIYFVTDRELLHLVGTPAAERKGERAA"
     gene            5101..6171
                     /locus_tag="VF720_16095"
     CDS             5101..6171
                     /locus_tag="VF720_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX7880934.1"
                     /translation="MNRVHTFLEMALKQGGSDLHLVSGEVPRIRINGVLEPVRFRELT
                     REDMERLLAEVLTEGQRARLARDLSVDFAYTVPELGRFRVNAYHHARGLAAALRVVPD
                     SLPLLDDLGLPTQVRELVRQPKGLTLVTGPTGSGKSTTLASIVDHLNRTRRGHIITIE
                     DPIEFVHQYRSSVVTQREIGAHAASFAEALRDAVREDPDAILVGELRDIETISLALTA
                     AETGIQVLGTLHTNGAGRCVDRIINVFPARRQDQVRSMLADSLRMVVSQQLVRLADGS
                     ARKLVADVLVNTAAMASMIRSGNTHKLATIMQSGGRQGMQLLDNVLHEMLRAEAITGE
                     EAWEHAVDKAPFERHLGLDGAA"
     gene            6168..6932
                     /locus_tag="VF720_16100"
     CDS             6168..6932
                     /locus_tag="VF720_16100"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX7880935.1"
                     /translation="MMLPPFVLVIVAAAGAAVVAGFIIWRVPRLYRRRHLDAVRTLMA
                     LVDAGDVLTRGHSYRVSRLSLRLGRQLGMRGGGLAELEYAALLHDVGRIAIRADVLQK
                     PGPLSADERSLVQTHPDVGHDIVKGLGFFPGAAEIIRSHHEQPDGKGYPRALRGKDIP
                     LGARIIMVAAALEAMTVDRPYRRGMKPVEAMAELSRHAGTQFDADIVAALQFLHAEGT
                     LYDDLDTGELARLTHRWSPSGVDEELYERKANARRA"
     gene            complement(6953..10306)
                     /locus_tag="VF720_16105"
     CDS             complement(6953..10306)
                     /locus_tag="VF720_16105"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7880936.1"
                     /translation="MATFRPIILSTLFLACFSLVIQTPPCDAAKKGADKAAVKSVSRS
                     ESKAAKEEGPPTLAKANLDGLSFRSIGPAVTGGRVIDIEVNPRDHSEYYVASGHGSLW
                     KTTNRGVTFSPVFDGQSSFPIGAVTIDPSNPNTVWVGTGENNAHSFVVPGDGVYRSDD
                     GGKSWVNKGLKEMQQAGEIVVHPHDSKTVWVAAYGPHRTDGGDRGVFKTTDGGETWTN
                     VLHPSDHTGCRQLFMDPRNPDVLYAVAHQRQRYLTTVVSGGDESGIYKTLDGGTTWKK
                     LEGGLPQKMVGRIGMAISPVNPDRLYAVVEAKEKKDKGTYMSTDGGASWTRQSDYLTT
                     YPFYFQRLVCDTENIDRVYGLDVFNQVSTDAGKSWSRLGEDRKHVDNHALWIDPDDAR
                     HLISGCDGGVYESFDKAETWAFKANLPLAEIYKVTVDNAKPFYNVYIGTQDNNSLGGP
                     SRTMNSSGITNADWVFTQGGDGFQTQVDWADPNILYAQAQFGALVRFDRRTGERLYLR
                     GFEGQGESAYRFDWDTPLLVSRHDHKRLYHGANKLLRSDDQGASWREVSEDLTRGVPK
                     ELDRLMGRSWRADELVAKSSFAHIVTIAESPLDEKRIYTGSGDGLLYYTRDGGEHWQK
                     ATLPGLPEFARIHQVVASPTDANVAYAACHNFFAGDFSPFLYRTTDGGGTWTSINADL
                     PVERGCTYTVGVDEVDPNLLFVGTMGGVYVSNSPEVSWVKLSGGMPATALVMSLEIQR
                     DEHDLVVSTFGRGVYILDDYTPLRGLTSKRLEEPVALYQVSDAPMFVAADPMGFPGVG
                     FQGASYFSAPNPDIGAPITYYVKEDYKTLEKQRNEAEKKLQEEGKDVGYPAYDVFKKE
                     ATEEEPYLLFVISDAENHALRKIKRPIKAGLHRVTWDFRTSPVVPIKLDSGGEKAPWD
                     SPEQGFMAPPGTYQVSLYRFQDRTLTPVGSPQSFTCRPLHESSLSPSDQAALAAFNGK
                     VAALSRAILAADAHRGRLSEALPYLEAAAMSVPTHDGAQLAELSAIKARLKEINEELT
                     GDNILTKYEGQARMSLKGRTDLIVESLWSTTAAPTGTFERAYTEAHASFGNVLAELRQ
                     VHDRTRALEDALERAGAPYTPGRMPTWEP"
BASE COUNT         1639 a         3577 c         3446 g         1743 t
ORIGIN      
        1 gtcatcgacg aacgccgacg cgaggatttc gaactcttca caggttgtca tgagtcactc
       61 tccgatagga caggtccaag cgccgcgatc accttctgac gtgcccggta cacgttcatg
      121 cgcacccggc tcatcggcca cccgtgcagg tccccgattt ccttgtagct ccgttgttcg
      181 agcaccgcca ggcgaaacgc ctgcgcgagc gcggccggca acgtctccag cacctctccc
      241 acccggttgg tgaggcccga gaggtgagcc tcctcctccg gggaggggct cgcatccact
      301 gcctcccagt tccccgccga ctcgtcgttc accaggtcca ggagaaggag atgccgcttc
      361 ttccggctcc tgtcgatcat cacgcggtac gcgatcgtca tgagatacgg cttgacgagt
      421 gcatcatccc gcagtgccga tccgccccgg atcgcacgaa tgaaggtttc ctgcagcagg
      481 tcctccgcgt cgtcccgctt ccgaacccgg ctggtcagga aagcaagcac ggctccgcca
      541 tgctcttcgt aggcttcctt ccagaacgtc agggggaccc cactttcctc gaccaggcag
      601 ctcccgtgtg gcgttgcgct gggacggaat gactccggga attgttacag ggaatggaca
      661 acgagcgccc cccgggtatt gcgctgtcaa gatattgatg tcaaatgact tacggtatcc
      721 tcaacagccg ggagccgtac cctgttctcc atcccgatac tcctctatcc ggggcatgac
      781 gctaaagccc ccatcccttc cggtcgataa gtcccggtgg accatctcga actggaggaa
      841 tgacgtgagt actgctgcca gcgcacaggc gccggagccg ggcaggctgc ttcggatccg
      901 cgtgtcactc aatgggcgac cgctcaagag ccaggtcttc gacaaggatg tcgtgcttgt
      961 cggtcgtgac cccgagtgcg acgtcgtgct cgacaacacg ggtgtctcgc gacaccacct
     1021 caagttcgag ctcggaccga ccggtttcta ctcggtgacc gacctcggca gcgcgaacgg
     1081 cacgctgttc aacgagcagc cgctgaagaa ggacttcatc tactcgggcg gcgatgtcct
     1141 gcagatcgga aagttcaccc tctgggtcac gcgcgagtcg gatcgccggg ccgatgagcc
     1201 cgatcggcgg cccacaccgg aagccgaagc acggaccacg gtgctctcgg ccacggagct
     1261 gctcaagatc gagcaggagg cgcgccagcg ccggatggcc tcgatcaccg cgttgccgac
     1321 ggcggcgccg gtggcggaga agccccaggt tcgacgggaa tcgaaggctt cgccccggat
     1381 gactcacgcg gcggccctcc tggtcggact cctggccggg atgctggtgc agagactcct
     1441 ctcgctcgtc atcgggatct gacggccgcc ggccggccgg atcgacggag ccgcgagcga
     1501 tgaagctggt cgatcacctg caggcggggc gacacatcgc cgcgctccgg tcgcatcccc
     1561 acccctccga cccacgccgc atccgggccc gccggcagct gatcgcgctc ggccccgggg
     1621 cggtaccccc tctgctgggc gccctcaagt cgctcgatgt gcgggcggcg gccggcgaaa
     1681 tactgttcga gctgctcgac gaccggactc ttccattctt tctggaagcc ctggccaacc
     1741 cggagccggc gacagcccgg gtcgtgctcg acatcctcac ccggaaccag gggtacgacc
     1801 cgacgcagct cctcgacctg ttcgccgatt cgagcgtccc gaagtcacgg gtcgaagctc
     1861 tcctcgtggc gcagatgccc cggatccagc cgcggctcct cgtgacactg attccggaga
     1921 tgtcacgcga ggcccgggca ctcacgttcc gcctgctgga ccgccacgcg gacgccgccg
     1981 ccgtcctgga gtcgatccga ctggccgacg atccggactg gtggatccgc ttccagatgg
     2041 cacggctgct ggcgcgggtc gcgcatccgg atggagcgac cgtcctcatc cggttgcttg
     2101 ccgacgagaa ccgcggggtt cggctggagg ccacccgatc gctcggccgg ctgcgcgatc
     2161 cgcgcgccgt ccccgcactc tgtcgtacgt tgcgtgatgc cgacctccgc gtgcagaccg
     2221 gcgcgatcga cgccctgatc ggcatcggcc atgcgtcggc cgttccgcat ctcgtcgaag
     2281 tcctgatgga cgaatcggaa accgcgcgac gggccgccgt ggaggtcctg aacgaagtgg
     2341 ccaccgccga cgcgatcaag gatctcgtga tggcgctccg ggatgccgac tggtgggtcc
     2401 ggtcgcgcgc ggcggacgca ctcggcacgc tcggcgggcc tccggtggtc gatgccgtcc
     2461 tggccatcct ggacgatccg gacgagcagg tgcgccgcta tgccgtggag atcctgaacg
     2521 tggccaccga tacccgggcc gtcgagccgc tgatccgggc gctcgacgac accgactggt
     2581 gggtccggga acgggccatc gatgccctgg cccggacgcg cgactcacgg gcggtggagc
     2641 ccctggccgc gcagctcttt cgcgatgccg atgccgcggc gctctgcgtg tcggccctgc
     2701 gccggatcgg acatccctcg gcactgccga tcttcgagcg cctcattgac tccgagtcgg
     2761 cggagctccg ccgtggcgcc atcgaggggc tcctcgaact cttcacggga gagctcagcg
     2821 cggatgagcg gcggcgcgtg gtggcactcc tcgccgatgt cggcgtggat ccgacccggc
     2881 ggggatcgcc gatccaggaa tcccgcccga cggtggcccg cacgccgggc aactccagcg
     2941 gcgaacggcc ggcgtcggcc acgggcgaga tgccggccgc accggtcact ccttcaaacg
     3001 cgccggtaac gcccgccaat gccacgccgc gaatcgcgcc cgactcccct tcgctgccgg
     3061 ccgccccgag ggtcttccgt gacgtcacgc agctcccgcc cggggaggtg ctcgtcgatc
     3121 gataccgcgt ggtccgcaag gtcggcggcg gcggtttcgg gactgtctat ctcgtggaag
     3181 acgtgctgat ccgcgacgaa ctcgtcctca aggtcctgaa cccgcagttg acccacgacg
     3241 ccaccatgac ccgccggttc ctccaggaga tgcgggtgag ccgccggatc acgcatccga
     3301 acgtcatccg gacgcacgag ctcgtcgacc tcggcggttg gcacgccatc gccatggaat
     3361 acttcccgag ccgcgacctc ggcgacgtat tgaagagcga agggcggatg tcgccggaac
     3421 gcgccctcgc catcggaatc caggtgtgcg aggcgctggc cgcggcgcac gagcgtggga
     3481 cgattcaccg cgacgtgaag cccggaaaca tcctcatcga cgagaacgac caggtgaagg
     3541 tcgtcgactt cggcctcgcg gcgatcggac agcgcagcgg ctcgagactc accatgagcg
     3601 gcacgctgct cggaacgccg gagtacatgg cgccggagca gatcacggac gggtcggccg
     3661 atgcccggag cgacatctac tcgctcggcg ccgtgctgta cgaaatgttg agcggggttt
     3721 ccccgttcgc gggtgagaac acggcccatg tcctcttcca gcacatcgag ggagacatcc
     3781 gcccgatcag cgaggcggcg cccgggcttc ccgaggggct cgacgcggtc gtcatgagca
     3841 tgctcgcgcg ggcccaggcc gatcggcccg atacggcgaa cgggctggcg gagcggctgc
     3901 gggccattcg cgcggttcag ggcggtgccg acggagccgg tcgggtggcg gccgcatgac
     3961 gtcgtcgttc cgttcccggg aggcgggtcg ccggatcgac gcgtttctcg acctcggccg
     4021 ggagcacggg gcctccgata tccacctgag cgccgggctt cccccgctcg tccggaccga
     4081 cggcgagctc ctccccgccc cgcatcggcc gctggccgac gacgagatcg ccgcgctgat
     4141 cgacgagatc ctcaacgagg accagcagcg catcttcgcg gaacgcggtt cgaccgatct
     4201 ctcctatcag tcggcccggg gtgggcgcta tcgcatcaac atctgccggc atctccacgg
     4261 gatcgcggcc atctgccggg tgatcccctc cgacgtgccg cgcctggctc accttggact
     4321 gccgtcgccg gtggcccgcc tgaccgcgtt ccggtcgggc ctggtgctcg tcaccgggcc
     4381 gacgggaacg gggaagtcga cgacgctcgc ggccctggtc aacgagatca accagaacaa
     4441 gagcctcaac atcctgacgc tggaagaccc ggtggaattc ctgcacacct cggcccgctc
     4501 ccaggtggtg cagcgggaga tcggcaccga cgttccgtcg ttcgaggccg gcctgcgcgc
     4561 cgcgctccgc gaagatcccg acgtgatcct ggtcggtgag ctccgcgatc cgaccaccat
     4621 ctccctggcg ctcgaagccg cggaaaccgg ccacttcgtc ctcggcacgc tgcacacgcg
     4681 cggggcccac cagacgatcg accggatcgt ggacgcctac cccgccgagg cccaggcgca
     4741 ggtgcgtcac accctggccg acaacctgcg gtgcgtcctg agccaggagc tcgtgaaggc
     4801 cgccgacggc cgggggcgac gggtggcgat ggagatcatg gtcggcacgc cggcggtggc
     4861 ccagctcatc cgcgacggca agacgttcca gctccagacg gccatcgcca ccggccgccg
     4921 cgtcggcatg cagctcatgg acctggcgct catcgcgctg gtccgcgcgg gcgagatcga
     4981 cccgaacgaa gccttcctcc gggccgccga caagcgcgag ttcatctact tcgtgaccga
     5041 cagggagctc ctccacctgg tcgggacgcc ggcggccgaa cggaagggag agcgggccgc
     5101 atgaaccgcg tccatacctt cctggagatg gcgctcaagc agggaggatc cgacctccac
     5161 ctggtctcgg gcgaagtgcc gcgcatccgg atcaacggcg tgctcgagcc ggtccgcttc
     5221 cgggagctca cgcgcgagga catggagcgc ctcctggccg aggttctcac cgaggggcag
     5281 cgggcgcggc tggcgcgtga cctgtcggtc gacttcgcgt acacggtgcc ggagctcggc
     5341 cggttccgcg tgaatgccta ccaccatgcg cggggactgg cggccgcgct gcgcgtcgtg
     5401 cccgactcgc tgccgctcct cgacgatctg ggattgccga cgcaggtccg ggagctggtc
     5461 cggcaaccga aaggcctgac gctggtgaca ggcccgaccg gctcgggaaa gtcgacgaca
     5521 ctggcctcga tcgtcgatca cctcaaccgg acacgccggg gccacatcat caccatcgag
     5581 gacccgatcg agttcgtgca ccagtaccgg agcagcgtgg tcacgcagcg ggagatcggc
     5641 gcccatgcgg ccagcttcgc cgaggcgctc cgggatgctg tccgggagga tcccgacgcc
     5701 atcctcgtgg gcgagcttcg cgacatcgag accatcagcc tggccctcac cgcggcggaa
     5761 acgggcatcc aggttctcgg gacgctgcac accaacggcg ccggccgttg cgtcgaccgc
     5821 atcatcaacg tcttccccgc ccggcggcag gaccaggtgc gatcgatgct ggccgactcg
     5881 ttgcgcatgg tcgtttccca gcagctggtg cgcctggccg acggcagcgc ccgcaagctc
     5941 gtggccgacg tcctggtgaa cacggcggcc atggcctcga tgatccggag cggcaacacc
     6001 cacaagctcg ccacgatcat gcagtccggg gggcgccagg ggatgcagct gctcgacaac
     6061 gtccttcacg agatgctgcg tgccgaggcg atcaccggtg aggaagcctg ggagcacgcc
     6121 gtggacaagg cgccgttcga gcgccacctc ggactggacg gggcggcatg atgcttccgc
     6181 cgttcgtcct ggtgatcgtc gcggctgccg gtgcggccgt ggtggccggt ttcatcatct
     6241 ggcgcgtgcc gaggctctac cggcgtcggc atctcgatgc cgtccgcacc ctgatggcgc
     6301 tcgtcgatgc cggcgacgtc ctgacccgcg gtcattccta ccgcgtctcc cgcctgtcgc
     6361 tgcggctcgg gcggcagctc ggaatgcgcg gtggcgggct ggcggaactc gagtacgccg
     6421 cgctcctgca cgatgtcggc cggatcgcca tccgcgccga tgtcctgcag aagccgggcc
     6481 ccctgtcggc cgatgagcgg agcctggtgc agacgcatcc ggacgtcggg cacgacatcg
     6541 tgaaggggct cggcttcttc ccgggggcgg cggaaatcat ccgctcccac cacgagcagc
     6601 cggacgggaa ggggtatccc cgcgctcttc gcggcaagga catcccgctg ggggcccgca
     6661 tcatcatggt tgccgcggcg ctcgaggcca tgaccgtcga tcgcccctac cgcaggggaa
     6721 tgaagccggt cgaggccatg gccgaactct cacgccatgc cggcacccag ttcgatgccg
     6781 acatcgtcgc ggcgcttcag ttcctccacg cggagggaac gctctacgac gacctggaca
     6841 cgggcgagct cgcgcggctg acccacaggt ggagcccctc aggcgtcgat gaggagttgt
     6901 acgaacgaaa ggccaacgct cgaagggcct gacaagactc cgggccggcc cctcacggct
     6961 cccaggtcgg catccgcccg ggggtatatg gagcccccgc ccgttccagg gcgtcctcca
     7021 gtgcgcgggt ccggtcgtgc acctgccgca gttcggcaag cacgttgccg aaggaggcgt
     7081 gtgcctcggt gtaggcacgt tcaaaggtcc cggtcggggc ggcggtcgtg ctccagagcg
     7141 attcgacgat caggtcggtc cgccctttca gggacatgcg ggcctgccct tcgtacttgg
     7201 tcaggatgtt gtcgccggtc agctcctcgt tgatctcctt gaggcgtgcc ttgatggcgg
     7261 acagctcggc gagctgcgcg ccgtcgtgcg tcggcacgct catcgccgcg gcctcgaggt
     7321 acgggagggc ctcgctcagc cggccgcggt gcgcgtcggc ggccaggatg gcccgcgaga
     7381 gggccgccac cttgccgttg aacgcagcga gcgcggcctg gtccgacggc gagaggctcg
     7441 actcgtggag cggccggcag gtgaacgact ggggtgaccc gacgggcgtc agcgtgcggt
     7501 cctggaagcg gtacaggctc acctggtacg tgccgggcgg ggccatgaat ccctgttcgg
     7561 gagaatccca gggggctttc tctccaccgg aatcgagctt gatgggcacc acggggctgg
     7621 tccggaaatc ccacgtcacc cggtgcagtc cggccttgat cggacgcttg atcttccgga
     7681 gcgcgtgatt ctcggcgtcg ctgatcacga agaggagata cggctcctcc tcggtggcct
     7741 ctttcttgaa gacgtcatat gccgggtagc caacatcctt cccttcttcc tgcagcttct
     7801 tttccgcttc gttccgctgc ttttcgaggg tcttgtagtc ctccttcacg taatacgtga
     7861 tcggcgcgcc gatgtcggga ttcggggcgg agaaatacga cgctccctgg aatccgacgc
     7921 ccgggaaacc catcgggtcc gccgcgacga acatcggcgc gtcggaaacc tggtagagcg
     7981 ccaccggctc ctcgagtctc ttcgacgtga gtccgcggag cggcgtgtag tcgtcgagga
     8041 tgtacacccc ccgcccgaag gtggagacga cgaggtcgtg ctcgtcccgc tggatctcga
     8101 ggctcatgac cagggccgtg gccggcatcc cccctgacag cttgacccac gagacctccg
     8161 gcgaattcga cacgtagacg ccccccatcg tcccgacaaa gaggaggttc ggatcgacct
     8221 cgtccacccc gaccgtgtag gtgcacccgc gctcgaccgg aaggtcggcg ttgatcgagg
     8281 tccaggtgcc ccccccgtcg gtcgtcctgt agaggaaggg gctgaagtca ccggcgaaga
     8341 agttgtgaca ggccgcgtag gccacattgg catcggtcgg tgaggccacc acctggtgga
     8401 tccgggcgaa ctcgggcagc ccgggaagcg tggccttctg ccagtgctct cccccgtctc
     8461 gcgtgtagta gaggaggccg tcacccgaac cggtgtagat ccgcttctcg tcgagcgggg
     8521 attccgcgat cgtgacgatg tgggcgaaag acgacttggc cacgagctca tcggcacgcc
     8581 agctccgccc catcaggcga tcgagctcct tcgggacgcc gcgcgtcaag tcctcgctca
     8641 cttcccgcca gctggccccc tggtcgtcgg agcggaggag cttgttggcc ccgtggtaca
     8701 ggcgcttgtg gtcgtggcgc gacacgagga gcggcgtgtc ccaatcgaag cggtaggccg
     8761 actctccctg tccctcaaaa ccccggaggt aaagccgctc ccccgtcctc cggtcgaaac
     8821 gaacgagcgc gccgaactgc gcctgggcat acaggatgtt cgggtcggcc cagtccacct
     8881 gcgtctggaa gccgtcgccc ccctgggtga agacccagtc ggcgttggtg atcccggagg
     8941 agttcatcgt ccgcgaggga ccgcccaggc tgttgttgtc ctgggtgccg atgtacacat
     9001 tgtagaaggg cttggcattg tcgacggtga ccttgtagat ctcggccagc ggaaggttgg
     9061 ccttgaacgc ccacgtttcc gccttgtcga acgactcgta cacgccgccg tcgcacccgg
     9121 agatgaggtg ccgcgcgtca tccgggtcga tccagagggc gtggttgtcc acgtgtttcc
     9181 tgtcctcgcc gaggcgcgac cagctcttgc cggcgtcggt cgacacctgg ttgaagacat
     9241 cgaggccgta cacccggtcg atgttctccg tatcgcagac caggcgctgg aaatagaagg
     9301 gataggtggt caggtagtcg ctctggcggg tccagctggc gccgccatcg gtgctcatgt
     9361 acgtcccctt gtccttcttc tccttggcct ccaccaccgc gtagagccga tctgggttca
     9421 ccggcgagat ggccatgccg atccggccga ccatcttctg cggcaggcct ccctcgagtt
     9481 tcttccaggt cgtgccgcca tccagggtct tgtagatgcc gctctcgtcg ccgcccgaga
     9541 cgacagtcgt gaggtagcgc tggcgctggt gcgccaccgc gtagagcacg tcgggattgc
     9601 ggggatccat gaagagctgc cggcatccgg tgtgatcgct cggatgaagg acgttcgtcc
     9661 aggtttcacc cccgtccgtc gtcttgaaga cgccccggtc gccgccgtcc gtgcgatgcg
     9721 gtccgtaggc cgccacccac accgtcttcg aatcatgggg atgcaccacg atctcgccgg
     9781 cctgctgcat ttccttgagc cccttgttca cccagctctt gccgccgtcg tcgctccggt
     9841 agacgccgtc acccggcacg acgaaggagt gggcgttgtt ctcccccgtc cccacccaca
     9901 ccgtgttcgg attggacggg tcgatcgtga ccgccccgat ggggaacgac gactgtccgt
     9961 cgaacacggg agagaaggtc acgccgcggt tcgtggtctt ccagagtgaa ccgtgtccgg
    10021 aagccacgta gtactcggag tgatcccggg ggttgacctc gatgtcgatc actcgtccac
    10081 cggtgaccgc cggcccgatg ctccggaacg aaaggccatc gagattggct ttcgcgagcg
    10141 tcggggggcc ctcttccttg gccgccttgc tttccgacct ggagacggat ttgacggcag
    10201 ccttgtcggc acctttcttc gcggcgtcac acggaggcgt ctgaatgacc agggaaaaac
    10261 aggcgaggaa aagcgtgctg aggatgatcg gacgaaacgt ggccatggcg ttcgggctcc
    10321 gggtgatcgg tgatctggtg tgagccggga tactacgacg tccgcgtccg gccgccgacc
    10381 ccgactcttt acgttacgat cacga
//
LOCUS       DASVOD010000751         5179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_674833, whole genome shotgun sequence.
ACCESSION   DASVOD010000751 DASVOD010000000
VERSION     DASVOD010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5179
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674833"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF720_16110"
     CDS             complement(<1..405)
                     /locus_tag="VF720_16110"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HEX7880937.1"
                     /translation="MPGARCRLDADRHPSRLSRVFPTRLILISIFVLVGAATAQTPPS
                     PAPPASTPPADRTVRTLYLIRHGIYDSADERPDSVGKALTPLGIAQIRLAAARLRGMP
                     VTFTSIRSSRLTRAWESARVLAADFPNLTVRPT"
     gene            complement(539..4561)
                     /locus_tag="VF720_16115"
     CDS             complement(539..4561)
                     /locus_tag="VF720_16115"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX7880938.1"
                     /translation="MPSWRPIRSAAVLALLHIAASVLPARAAEPIGGMPFAGEEGIQE
                     TIAEIMNRELTMPRIDEPTDQEAELTQPDRTHLPQNPLAPKVSSVPEMPAGFGANDGG
                     PGLFAPQTIGTNFTGATLAGTNATLSFPPDCMGAVGPTQYFMFVNGRLVTFNKATGVA
                     DGVINADPDVFFTSVINGSSTSDPRIRYDRLTGRWFLVIINVSTPNRILIAVSDAASN
                     GTLTLGSSFTFFFINIATAPPSISNTCFADYPTLGIDNNALYIGTNNFCGSPTQTFNS
                     CDGFVIRKSSILGAGPIVTPVFRGLVASSAAAGPYTPQGVDNYDPSATEGYFIGVDNA
                     TFSTLMFRRVSTPGGTPTISGNISITTPTTTFPENVPHLGNTGGTNGRLSALDDRLFA
                     AHIRNQQLWTAHNIEVNASGVASTTGSRDAARWYHLNVPVGSGTPTIVQSGTVFDNAA
                     TNPNYFWIPSILPSGQGHVAMGLSRAGNASRADAATVGRLSGDAGGTMQAPALVTASS
                     TAYNPPSDPGGTGGRRWGDYSYVSLDPIDDMTMWQVQMFCDAANSYGVRITRLIAPPP
                     ATPSALADITAGANPVNVTLTGLQVSGSGFYDPGANLPGGVPGFNHLTATITNGTATG
                     TPPTVVSATYVSPTSVNLVLNASAATASIGAERYRLTITNPDGQTAFGDVVKVVGGTP
                     TVSIATGPTTNEGNAGLTNFNFTVNLSATTPNPVTVNYQTSDGTATVADNDYVAANSS
                     IIIPANSPSGTITISVKGDTRFEPNETFGVTLTGATNATLGSPVLATGTIQNDDAVPV
                     ISIGNALVAEGNAGMTVLSFPVTLSNPSASIVSATFQTADGTALISDGDYSSNMGSVS
                     FPANSTADTIDVNVTADTRFEDDETLTVTLSAPVNGTLGTPVGTGTIQNDDSVPTISI
                     DDVSLNEGDAGATIFQFTATLSAISGKTASASYTTTEGTATLANNDFDASSGPLSFPP
                     GTVSIPVMIQVNGDVTLESNETFTVDITPLTNVAAGDVSGAGDILNDDDVPFLEVDSV
                     SHLETDAGAVVYRFTVSLSNATDQPVSVQWNTADGSATTADNDYVSNGGTLNFPPKVL
                     TQTFDVFANGDVCGENDEVFDVVLSSPVNAAIGTGTGAGTIQNDDENVDPSVTVGFPN
                     GAEILQVGTQVTIQWNASDNVGVTGVDIRLSRDSGATFPEYLATNIPNTGSFLWTVTG
                     PGSLVPEEYLMVVAHDAHFNDGNDISDNAFQIVAPTSSVPGLGAVASFAIASVRPNPT
                     RGATSIEFAVPREGRVRISIVDLQGREVAVLQDNTLSTGRYQTNWDGTTSRGPAPMGV
                     YFVRAEGNGTVVTERFVVAR"
     gene            4787..>5179
                     /locus_tag="VF720_16120"
     CDS             4787..>5179
                     /locus_tag="VF720_16120"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7880939.1"
                     /translation="MLNRPVTNALPASREGDRVLRGHWQDVAGHWRDTLPERLWRAHA
                     DRVNADWLAARLPARPVTTLLKTDLFDEAATGGLYPLLSRHARVVVGMDISSTTARGA
                     GQRARGIAATCADARRLPFADGSFDVVVS"
BASE COUNT          770 a         1564 c         1922 g          923 t
ORIGIN      
        1 cgtggggcgc accgtcagat tcggaaagtc ggcggccagc acgcgcgccg attcccacgc
       61 gcgcgtgagc cgactggagc ggatcgaggt gaacgtgacc ggcatccccc tgagccgcgc
      121 cgccgccaga cggatctggg cgatgccgag cggagtgagc gccttcccca cgctgtccgg
      181 ccgttcgtcg gcactgtcgt agatgccgtg gcggatcagg tagagcgtgc gaacggtgcg
      241 gtcggcgggg ggtgtggatg cgggcggtgc gggtgagggc ggcgtctggg cggtggcggc
      301 gccgacgagg acgaagatcg agatgaggat gagtcttgtc gggaatacgc gggacagcct
      361 ggagggatgt ctgtcagcat cgagtcggca gcgggctcct ggcatgggtc gtattcctcc
      421 gtggcggcac gatagcactg gacgacagca agatggcgga ccggccgcca ggccgatccg
      481 ccaccgattc catcttttcg acattcgtcg tcgggtcgac tgcgtcgatc cttgcgcgtt
      541 accgcgccac gacgaaccgc tccgtgacca ccgtgccgtt cccttccgcg cggacgaagt
      601 agacgcccat cggggccggg ccgcgtgagg tcgtgccgtc ccagttggtc tggtagcggc
      661 cggtgctgag cgtattgtcc tggaggacag ccacttcacg cccctgcagg tcgacgatgc
      721 tgatccggac gcgcccttcg cgcggaacgg cgaattcgat gctcgtagca ccgcgggtcg
      781 ggttcgggcg gacggacgca atggcgaacg atgccaccgc accgaggccg ggcacgctcg
      841 aggtcggcgc cacgatctgg aaggcgttgt ccgaaatgtc gtttccgtcg ttgaagtgcg
      901 cgtcatgcgc caccaccatc aggtactcct ccggcaccaa cgacccggga ccggtgaccg
      961 tccagaggaa gctgccggtg ttgggaatgt tggtggcaag gtactcgggg aaggtggccc
     1021 cgctgtcgcg cgagaggcgg atgtccacgc cggtcacgcc cacgttgtcg gaggcgttcc
     1081 actggatcgt cacctgggtg ccgacctgga ggatctcggc gccgttcggg aagcccaccg
     1141 tcaccgacgg gtcgacgttc tcgtcgtcgt tctggatggt gccggcgccg gtacccgtgc
     1201 cgattgccgc gttgaccggg ctcgacagca ccacgtcgaa cacctcgtcg ttctcgccgc
     1261 agacgtcgcc gttggcgaag acgtcgaacg tctgcgtcag caccttgggc gggaagttca
     1321 gcgtgccgcc gttcgacacg tagtcgttgt ccgcggtggt ggccgaaccg tccgccgtat
     1381 tccactggac cgaaaccggc tggtcggtgg cgttcgagag cgacaccgtg aaccggtaga
     1441 ccacggcgcc cgcatcggtc tcgaggtggc tcaccgagtc cacctcgagg aacggcacgt
     1501 cgtcgtcgtt caggatgtca ccggcgcccg acacgtcgcc ggcggccaca ttggtgagcg
     1561 gcgtgatgtc gacggtgaac gtctcgttcg actcgagcgt cacgtcgcca ttgacctgga
     1621 tcatgaccgg aatggacacc gtacccggcg ggaagctcag ggggccggag gacgcgtcga
     1681 agtcgttgtt cgccagcgtg gcggttccct cggtggtcgt gtacgaagcg gaagcggtct
     1741 tgccgctgat cgcgctcagt gtggccgtga actggaagat cgtggcgccc gcatccccct
     1801 cgttgagcga cacgtcgtcg atcgagatcg tcggcacgct gtcgtcgttc tggatcgtgc
     1861 cggtgccgac cggggtgccg agcgtgccgt tcaccggggc gctcagcgtc acggtgaggg
     1921 tctcgtcgtc ctcgaagcgg gtgtcggcgg tcacgttcac gtcgatcgtg tcggccgtac
     1981 tgttcgccgg gaagctcacg ctacccatgt tcgaggaata gtcgccgtcg ctgatgagcg
     2041 ccgtgccgtc ggccgtctgg aaggtggccg acacgatgct cgcgctcggg ttcgacaggg
     2101 tgaccgggaa ggagaggacg gtcatgccgg cgttcccctc ggccaccagg gcgttgccga
     2161 tgctgatgac cgggacggcg tcgtcgttct ggatggtgcc ggtcgcgagg accggtgagc
     2221 cgagggtggc gttggtggcc ccggtcaggg tcacgccgaa cgtctcgttc ggctcgaacc
     2281 tggtgtcacc cttcacgctg atcgtgatcg tccccgaagg agagttggcg gggatgatga
     2341 tcgagctgtt ggcggccacg tagtcgttgt ccgccacggt ggcggtgccg tcggaggtct
     2401 ggtagttcac cgtgacgggg ttcggcgtgg tggccgacag gtttacggtg aagttgaagt
     2461 tggtcaggcc ggcatttcct tcgttggtgg tcggtcccgt cgcgatcgac acggtgggcg
     2521 tgccaccgac caccttcacc acgtcgccga aggccgtctg gccgtcgggg ttcgtgatgg
     2581 tgaggcggta acgctcggca ccgatcgagg cagttgcggc cgaggcattg agcaccaggt
     2641 tcaccgacgt gggactcacg taggtggccg acacgacggt cggcggcgtg ccggtggccg
     2701 tgccgttggt gatcgtggcc gtcaggtgat tgaaacccgg aacgccgccc gggaggttgg
     2761 cgcccggatc gtagaacccg gatccggaga cctgaagtcc cgtcagcgtt acgttgaccg
     2821 gattggcgcc cgcggtgatg tcggccagcg cgctcggcgt ggccggcggc ggggcgatca
     2881 gcctggtgat gcgcacgccg tacgagttgg cggcgtcgca gaacatctgc acctgccaca
     2941 tcgtcatgtc gtcgatcggg tcgagcgaga cgtagctgta atcgccccag cggcgaccgc
     3001 cggttccgcc cggatcgctc ggcgggttgt aggcggtgga gctggcggtg acgagcgccg
     3061 gcgcctgcat ggtgcccccg gcatcgcccg aaaggcggcc gaccgtggcg gcatcggcac
     3121 gcgaggcgtt gccggcgcgg ctcaggccca tggctacgtg cccctggccg gagggcagga
     3181 tcgacggaat ccagaagtag ttcgggttgg tggccgcgtt gtcgaagacg gttccggact
     3241 ggacgatcgt gggggtgccc gagccgaccg gcacgttcag gtggtaccag cgcgccgcgt
     3301 cgcggctgcc ggtggtggag gccacgcccg aggcgttcac ctcgatgttg tgggccgtcc
     3361 agagttgctg gttgcggatg tgagccgcga agaggcggtc atcgagcgcc gagaggcggc
     3421 cgttggtgcc tccggtgttg ccgaggtgcg gcacgttctc ggggaaggtt gtcgtcggcg
     3481 tcgtgatcga gatgttgccc gaaatcgtcg gcgtgccgcc cggcgtcgac acgcggcgga
     3541 acatcagggt gctgaaggtg gcgttgtcca ccccgatgaa atacccctcg gttgcggacg
     3601 gatcgtagtt gtccaccccc tgcggcgtgt acgggccggc ggccgccgaa ctggccacca
     3661 ggccccggaa cacgggggtg acgatcgggc cggcgcccag gatcgagctc ttgcggatca
     3721 cgaagccgtc gcatgaattg aaggtctgcg tcggcgagcc gcagaagttg ttggtgccga
     3781 tgtagagggc gttgttgtcg atgccgagcg tcgggtagtc ggcgaagcag gtgttcgaga
     3841 tcgagggggg cgccgtggcg atgttgatga agaagaaggt gaaggacgaa cccagcgtga
     3901 gggtgccgtt gctggcggcg tcgctgaccg cgatcaggat gcggttgggc gtcgacacgt
     3961 tgatgatgac gaggaaccac cggccggtga gccggtcgta gcggatgcgc ggatcgctgg
     4021 tcgaggagcc gttgatgacc gacgtgaaga agacatcggg atcggcgttg atgaccccgt
     4081 cggccacgcc cgtggccttg ttgaaggtga ccagtcgccc gttcacgaac atgaagtact
     4141 gggtggggcc caccgcgccc atgcagtcgg gcgggaacga cagcgtcgcg ttggtgcccg
     4201 ccagcgtggc gccggtgaag ttcgtgccga tggtctgcgg cgcgaagagc ccggggccgc
     4261 cgtcgttggc gccgaagccg gccggcatct cgggcacgga gctcaccttg ggcgccagcg
     4321 ggttctgcgg caggtgggtc cgatccggct gtgtcagttc ggcttcctgg tccgttggct
     4381 cgtcgatccg gggcatggtg agctcccggt tcatgatctc ggcgatggtc tcctggatac
     4441 cctcttcacc cgcgaacggc atgccgccga tcggttcggc ggctcgggcc gggagcacgg
     4501 aggcggcgat atggaggagg gcaagtacgg cggcagagcg gattgggcgc caggatggca
     4561 ttgagcatct cccgaggtgc ttcgaaggga agggcagcgg aacgggtgtt ccgggcggga
     4621 ctggtgaaat tccccatgag tttacggtga tccacttctg aagtccacga aatccttcgc
     4681 gtgtggcggt ttaccccact tcgggcgagt cggttgttga caggcaccat gcgagtggcg
     4741 aaagtcccgt cggcccgatt gctttccacg cctgccgccg gaggaagtgc tgaaccgtcc
     4801 cgtcaccaac gcgttgcccg cctcccgcga aggggacagg gtgctgcgcg gccactggca
     4861 ggatgtggcc ggccactggc gggatacgct gccggagcgg ctctggcggg cgcacgccga
     4921 ccgcgtcaac gccgactggc tggcggcgcg attgcccgcc cgacctgtca ccactctcct
     4981 caagacggat ctttttgacg aagcagccac cgggggtctc tatcccctgc tgtcccggca
     5041 cgcccgcgtg gtggtgggca tggacatttc ttccacgacg gctcgcggtg cgggacaacg
     5101 cgcgcgcggc atcgccgcca cctgtgccga cgcgcgacgg cttccctttg cggacgggtc
     5161 gttcgacgtg gtcgtttcg
//
LOCUS       DASVOD010000752         2157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_260416, whole genome shotgun sequence.
ACCESSION   DASVOD010000752 DASVOD010000000
VERSION     DASVOD010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260416"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF720_16125"
     CDS             <1..216
                     /locus_tag="VF720_16125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880940.1"
                     /translation="ENEVFLNGGVEYSYNKLISLRTGYIFDDPGEIKDFTFGLGVAYK
                     ALSFDYASIPQFEELDRVSKFSLSVRF"
     gene            470..>2157
                     /locus_tag="VF720_16130"
     CDS             470..>2157
                     /locus_tag="VF720_16130"
                     /inference="COORDINATES: protein motif:HMM:NF017368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A domain-containing protein"
                     /protein_id="HEX7880941.1"
                     /translation="MAHELRIVDGTATPDAPGRDGTAATGTDTDFAPHDERWSSRFAF
                     SSIDTLRVAMIRVEFETDRAGDSTTTVDGKFDRRTGIVAAVDPPPHEGAYFDAHGTAL
                     ANYYRAQSNGHLEVDVHVFPNDPGAAYRLTDTADYGPWEILSDNVDVAEQAQRLIKDG
                     LEAAEASGEVDFPSFDAFILVHAGADFQSDVNQDSRYDIPTFVLDFDESDTLEVGGKR
                     IYRCMVLPETTTQDGFSGALNGVLAHEFGHILGLPDLYNTRTGFPMVGWFSVMDSGHN
                     IGAILVDPDSTEVEVFGALPTSFDAWCRQQLFGNADPDAVNQPGNGWLVPRITTVEES
                     LHTDLGAVLADHRLIRVPIHDSEYYLVENRELEYDGNGFPIIRADPATGVILGPIADS
                     TLSNPDGALEYDALLPSGGLLIWHIDDRVLYQDLQDPLGVNTNINRRGIKLVEADAIE
                     DQGRRNYGTPWDPFYAGNNPFFGPWSLPATTTNDGQFSRIEIGTTSPPDLSMDVTVRR
                     PTALAGWPIGLAGDIVVTSTGLLDVTGDGSPEMLFTFGRDIFAVEGKGGRPYPPL"
BASE COUNT          365 a          791 c          647 g          354 t
ORIGIN      
        1 gagaacgaag tgttcctgaa cggcggtgtg gagtactcgt acaacaagct catctcgctc
       61 cgcaccggtt acatcttcga cgatccgggc gagatcaagg acttcacgtt cggactcggc
      121 gtggcctaca aggcgctgtc gttcgactac gccagcatcc cgcagttcga ggagctggac
      181 cgcgtcagca agttctcgct gtcggtacga ttctgatttg atccctgctt gcctcccgcc
      241 ggcgggcttg aggacagccc ggctgacggc aattctcgcg gccggactgg ccctggcggg
      301 aggcacgcgg gttcttgccc gcgaggtccg gctcaagccg gatcgcgccg ccgaaacggc
      361 ggccagtcgt cgacgcctcg accactgggc ggggttcccc tgtttcttgg gcactcgccg
      421 gctccccgaa ctccgaattc ccaccgcgcc gacccgggcc ggcaatggca tggctcacga
      481 gcttcggatc gtggatggaa cggccactcc cgacgctccg gggcgcgacg gcaccgccgc
      541 aacgggcacc gataccgatt tcgccccgca cgacgagcgc tggtcgagcc gcttcgcctt
      601 cagctcgatc gacacgcttc gcgtggccat gatccgcgtg gaattcgaga ccgaccgcgc
      661 cggcgattcc accaccaccg tggacggaaa attcgaccgg cgcaccggca tcgtggccgc
      721 cgtcgatccc ccgccgcacg aaggcgccta cttcgacgcg cacggaacgg ctctggccaa
      781 ctattaccgt gcgcaatcca acggccacct cgaggtggac gtccatgtct tcccgaacga
      841 tccgggcgcg gcctatcgcc ttaccgacac ggccgactac ggtccgtggg agatcctgtc
      901 ggacaacgtc gatgtcgccg aacaggccca gcggttgatc aaggacgggc tcgaggcagc
      961 ggaggcctcg ggagaagtcg acttcccctc gttcgacgcg ttcatcctcg tgcacgccgg
     1021 cgccgacttc cagagtgacg tgaaccagga ttcgcgctac gacatcccga ccttcgtgct
     1081 ggacttcgac gagtccgaca cgctcgaggt cggagggaag cggatctacc gctgcatggt
     1141 cctgccggag acgaccacgc aggacggttt ttccggcgcc ttgaacggcg tcctcgccca
     1201 cgaattcggc cacatcctcg gcctccccga cctgtacaac acgcgtaccg gattcccgat
     1261 ggtgggatgg ttctcggtga tggactccgg ccacaacatc ggcgccatcc tcgtcgatcc
     1321 cgactcgacc gaggtggagg tgttcggggc gctccccacc tcgttcgacg cctggtgccg
     1381 ccagcaactc ttcggcaatg ccgaccccga cgcggtgaac cagccgggca acggctggct
     1441 ggtgccccgc atcaccacgg tggaggaatc actccacacc gacctgggcg cggtacttgc
     1501 cgaccaccgc ctgatccgcg tgccgatcca cgactccgag tactacctgg tcgagaaccg
     1561 cgagctggag tacgacggca acggcttccc gatcatccgt gccgaccccg ccaccggcgt
     1621 catcctgggg ccgatcgccg actccacgct cagcaatccg gacggcgcgc tcgagtacga
     1681 cgcgctcctc ccatcgggcg gtctcctcat ctggcacatc gacgatcgcg tcctctacca
     1741 ggacctccag gacccgctcg gcgtgaacac caacatcaac cggcgcggca tcaagctcgt
     1801 ggaagccgac gccatcgagg atcaggggcg gcgcaactac ggcaccccgt gggacccgtt
     1861 ctacgccggc aacaacccct tcttcggccc ctggtcgctc cccgccacca ccaccaacga
     1921 cggccagttc tcgcgcatcg agatcggcac gacctcgccg cccgatcttt cgatggacgt
     1981 caccgtccgc cgcccgacgg ccctcgccgg ctggcccatc ggcctggccg gcgacatcgt
     2041 cgtcacctcg accggcctcc tcgacgtgac gggtgacggt tcaccggaaa tgctcttcac
     2101 cttcggccgc gacatcttcg cggtggaagg gaagggaggc cggccgtatc cgccgct
//
LOCUS       DASVOD010000753         5954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_206773, whole genome shotgun sequence.
ACCESSION   DASVOD010000753 DASVOD010000000
VERSION     DASVOD010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206773"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF720_16135"
     CDS             complement(<1..192)
                     /locus_tag="VF720_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880942.1"
                     /translation="MTGPRNIPWIPLAGLAVLGALIAFVWSGLVATRPFVVESSAGEY
                     PYAAAARNLDSPRPVEPVIP"
     gene            complement(203..3487)
                     /locus_tag="VF720_16140"
     CDS             complement(203..3487)
                     /locus_tag="VF720_16140"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX7880943.1"
                     /translation="MTTHRASKSAPRASGRLSANDLMVLGLFFLSGASALVYEVVWSR
                     LFTFVFGGTASAIATVLSAYMAGLALGSWFFGRRIDRGGHPLKVYAILEAGIAVWAII
                     LPLVLHLLDGVYGGLYRSMHPGPTTLSLIRFAISFLVLLVPTILMGGTLPVLSRLLVD
                     DRSAVGLKAGLLYATNTVGAVAGTASAGFLLIPSLGMRTSTWVAIVFNVLVALLAVVL
                     ARRIPLPADGAPARSHTPEPAVVEATALPAKNTSITRAALVVYALSGFAALTYEVAWT
                     KVLSGALGTTTYAFSAMLTTFLLGLSLGAVLVARFAARFDAARLLAFFQLAIAVLALA
                     TLPLFGNLPILFIQAFKTWGPSWPAQLFSRFLLCAVTMLPPTLLMGAAFPLVARLYTD
                     GTQGLGKKVGEMYAANTVGAILGSFVAGFIFIPALGRQSTILVAVGVNLASAFLLYLA
                     LARAGRMTRGAWTLAGAFALVALALIPITARRWDPYLMASGAYVYADGYAREPDLKKP
                     LHEQRLLLFKEESEAILSVWRSQHVVSLRTNGKVEASSSGDMLTQKMISHLPIVYHKA
                     PEDVLMIGLASGISLGSVLAHPEVRKADCVEMLGGMRDVTDFFLKWNHDCLRDPRTNL
                     IINDGRNHLQLTREKYDVIISQPSNPWIVGIGSLFTREFFRLSAERLTDDGILCQWVQ
                     TYQMSRRDFGSILKTFHEAFPHVAVWMGVPGDIILLGSRQPLTLDWSRLNERLAVSTV
                     ASDLEVVGVNGTVSFLASYIGETRALEQLSAAAGPIVTDDNLLLEFSMPRNLYDPHVP
                     MMDHTLLIPVREPLTSTLTGGSPDSLRRLETRLLEGRARALQGVAVWQGGRNAETVAL
                     MEQALSFVPEDPLAISYLSQARNDIAIKLLEGGQTTQAMNEFRRAAALGSRSERALAL
                     NNLGLQHFSEDRPDSARDYWQRAVDLEPESPIIQFNLALVLDRLGESRGAAEHYRQVV
                     ALEPDNASSLNNLAWILAQDPATAGEAAELARRAVAAEPTDTNRDTYAWSMHKSGRSK
                     EAETLLREIVQRSPDLLEARYHLASVLKATGRTAEARSLYEEIVRRAAGNPLGADAAA
                     ELRNLP"
     gene            3762..5540
                     /locus_tag="VF720_16145"
     CDS             3762..5540
                     /locus_tag="VF720_16145"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX7880944.1"
                     /translation="MIRGWKAVSLLALPASMVLGCSSGPKDPTLATVGDHAIKLSEYT
                     AAWQGLPDAAKPDISTPEKRMAFLNDLINKEVMEMEARRRQPELEESQRRRLHRFAET
                     QLMNQLTTVEINNKIKVEDAEVRAVWNRMDREFHARHILVPTRDQALDIKKQLDGGAA
                     WEAMAAEHSKDPDNAQRGGDLGWVAAGVMVQPFEEALFSLQAGQISDPIQTRFGYHII
                     KVDEVRNVERPEFESVRERVYSLLLQQRSLARQEEFQKMLNEKAKPANQLDAIQLIDK
                     KYYFEVPAAQANDPYALLNANRMTPAFSKEELAMPVIKFADRPDFTIQDFNEALSWMP
                     PGLWPSGKGLEEIETLLRQMTRTKLYRDHAISLGIEQTPEYVRTIQKKESEMRVNGLY
                     YNGIVGSITITDTDKREFFDAHRDNYKIADRYRLSRIETPDSAVAQLAWRNWKTGKDF
                     EDVASMVKGLDPTAGATAATFEIPKGQSPPVDDLVFKGQVGDIIGPVHLESSVLESGQ
                     TLPPRWLVAKILDIKPERQMTYEEGESFVADHAKTARAEEELKKILDTAKVTFQVKID
                     EAAMARVTAPMIQEAPNPKKDAAANS"
     gene            complement(5589..>5954)
                     /locus_tag="VF720_16150"
     CDS             complement(5589..>5954)
                     /locus_tag="VF720_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880945.1"
                     /translation="LSALTCGVAGLVLGAVLVHYMSFYVGSLFLNDAGANDGLRLSLM
                     AWPIWSIIRVVGFIAGGVAMAWLFTTVIRRLKDKPARWPGPASHYLSLSLALVILDAL
                     LKALLAHHWQVTLLRAISY"
BASE COUNT         1098 a         1961 c         2049 g          846 t
ORIGIN      
        1 gggaatgacc ggctccaccg gcctcggtga atcgaggttg cgcgcggccg ccgcgtaggg
       61 atattccccc gcggacgact ccaccacgaa gggtcgcgtg gccacgaggc ccgaccagac
      121 gaaggcgatc agcgccccaa ggacggcgag tcccgcgagc ggaatccagg ggatgttcct
      181 cgggccggtc atggcaccct cctcagggga gattccggag ctcggccgcc gcgtcggcgc
      241 ccaggggatt tccggcggcg cggcgcacga tctcctcgta aagcgatcgc gcctcggccg
      301 tcctgccggt ggctttcagc accgacgcca ggtgatagcg cgcctcgagc aggtccgggg
      361 agcgctgcac gatttcgcgc agcagcgttt ccgcttcctt cgagcgcccg gacttgtgca
      421 tcgaccaggc gtacgtgtca cggttcgtgt cggtcggttc ggccgccacg gcccggcgcg
      481 ccagctcggc ggcctcgccg gcggtggcgg ggtcctgcgc caggatccag gccaggttgt
      541 tcagcgagga cgcgttgtcg ggttcgaggg ccaccacctg acgataatgc tcggcggccc
      601 ctcgcgactc cccgagtcga tcgagcacca gcgccaggtt gaactggatg atgggagact
      661 cgggctcgag atccaccgcc cgctgccagt agtcgcgggc cgagtccggg cgatcctccg
      721 agaagtgctg caggccgaga ttgttcaggg ccagcgcccg ttccgaacgg gagccgagcg
      781 ccgccgcccg ccggaattcg ttcatggcct gcgtggtctg cccaccttcg aggagcttga
      841 tggcgatgtc gttccgcgcc tgcgacaggt acgagatggc caggggatcc tccggcacga
      901 acgacagcgc ctgctccatg agcgccaccg tctcggcgtt gcgccctccc tgccagaccg
      961 ccaccccctg cagcgcccgc gcccgaccct cgaggagccg tgtttccagg cggcggaggg
     1021 agtcgggcga tccgccggtc agcgtgctgg taagcggctc gcggaccggg atcaggagcg
     1081 tgtggtccat catcggcacg tgcgggtcgt acaggttgcg cggcatcgag aattcgagga
     1141 ggaggttgtc gtcggtcacg atcgggccgg ccgccgcgct caactgttcc agggcgcgcg
     1201 tttcaccgat gtacgaggcc aggaaggaca ccgtgccgtt cacgcccacc acctcgaggt
     1261 cggaggccac ggtggacacg gccagtcgct cgttgagccg gctccagtcg agcgtcagcg
     1321 gctgccggct tccgagcagg atgatgtcgc ccggcacccc catccacacg gccacgtgcg
     1381 ggaacgcctc gtggaacgtt ttcaggatcg agccgaagtc gcggcgcgac atctggtagg
     1441 tctgcaccca ctggcagagg atgccgtcgt ccgtcaggcg ctcggcgctc aggcggaaga
     1501 actcgcgcgt gaagagggag ccgatcccca cgatccaggg gttcgacggc tgcgaaatga
     1561 tcacgtcgta cttttcgcgc gtgagctgca ggtgattgcg gccgtcgttg atgatgaggt
     1621 tcgtccgcgg gtcgcgcagg cagtcgtggt tccacttcag gaagaaatcg gtcacgtccc
     1681 gcatgccgcc cagcatctcg acgcagtcgg ccttcctgac ctccggatgg gccagcaccg
     1741 agccgagcga gataccgctg gccaggccga tcatgagcac gtcttccggg gccttgtggt
     1801 acacgatcgg caggtgggaa atcatcttct gggtgagcat gtcgcccgaa gacgacgcct
     1861 ccaccttgcc gttcgtgcgc agcgacacga cgtgctgcga ccgccacacc gagaggatgg
     1921 cctcggattc ctccttgaag aggaggaggc gctgctcgtg gagcggtttc ttgagatcag
     1981 gctcgcgggc gtagccgtcc gcgtagacgt aggcacccga ggccatgagg tacgggtccc
     2041 accggcgggc cgtgatcggg atcagggcga gggccaccag tgcgaacgcc ccggccagcg
     2101 tccaggcgcc gcgggtcatc cggccggccc tggccagggc caggtacagg aggaacgccg
     2161 atgccaggtt cacacccacc gccaccagga tggtggactg ccgccccagg gccgggatga
     2221 agatgaagcc ggccacgaac gagccgagga tcgcgccgac cgtgttggcc gcgtacatct
     2281 cgcccacttt cttcccgagc ccctgcgtgc cgtcggtgta gaggcgggcc accagcggga
     2341 aggccgctcc catgaggagc gtggggggga gcatcgtgac ggcgcacaat agaaagcgcg
     2401 agaagagctg ggccggccac gacggacccc acgtcttgaa cgcctggatg aagaggatcg
     2461 gcagattgcc gaagagaggg agcgtggcca gcgccagcac cgcgatggcc agctggaaga
     2521 atgccagcag gcgcgccgca tcgaaccgcg cggcgaaccg cgccaccagc acggcgccca
     2581 gcgaaaggcc gaggaggaac gtcgtgagca ttgccgagaa ggcgtaggtg gtggtgccga
     2641 gcgcccccga gaggaccttg gtccaggcca cttcgtaggt gagggccgcg aagccgctca
     2701 gtgcgtacac cacgagggcc gcgcgggtga tgctcgtgtt ctttgcgggg agagcggttg
     2761 cttccacgac ggccggctcc ggggtgtggc tcctggccgg cgccccgtcg gccggcaggg
     2821 ggatccgccg ggccagaacc accgccagga gcgccaccag cacgttgaag acgatggcca
     2881 cccaggtgga ggtgcgcatg ccgagcgatg gaatcagcag gaatcccgcc gaggccgtgc
     2941 cggccacggc acccacggtg ttggtggcgt acaggagccc cgccttgagc cccaccgccg
     3001 agcggtcgtc caccaggagg cgcgacagga cggggagcgt gcctcccata aggatcgtcg
     3061 ggacgaggag cacgaggaac gagatcgcga agcggatcag cgagagcgtg gtgggcccgg
     3121 ggtgcatcga gcggtaaagg ccgccgtaga cgccatcgag caggtgcagc accagcggga
     3181 ggatgatggc ccacaccgcg atgccggcct cgaggatcgc gtacaccttg agggggtgcc
     3241 cgccccggtc gatccggcgg ccgaagaacc acgaaccgag ggccagcccg gccatgtagg
     3301 cggagaggac ggtggcgatg gccgaggccg tgccgccgaa gacgaacgtg aagaggcggc
     3361 tccagacgac ctcgtacacg agggcgctgg ctccggagag gaagaagagc cccaggacca
     3421 tcaggtcgtt ggcgctcagg cgtccactgg cccggggggc ggatttcgag gctcgatggg
     3481 tcgtcaaggc acttccggtt ggtcaaggct ccgagacgga aaaggctccc gaatcgggtg
     3541 aaaagacgcg caaaagatta tcacccggga agttggccgg gcaacaaggg tgggtgcagg
     3601 gagcggcgcc gcgagcggat tctgttgcgg cccatccggg ggtcgtttac cattttgcac
     3661 gcattactgg ccccaccggc gtcggccggc caacgggcgg gacgcctcct tcgcgtccat
     3721 tgccgggtca tcccccggac gattccacag gagcttctcg catgattcgc ggttggaagg
     3781 ccgtttccct cctcgcattg cccgcctcga tggtcctcgg ttgctcgagc ggcccgaagg
     3841 accccacact ggccaccgtc ggcgatcatg cgatcaagct ttcggaatac acggctgcct
     3901 ggcaggggct gccggacgcg gccaagcccg atatctccac gccggaaaag cggatggcct
     3961 tcctgaacga cctcatcaac aaggaggtca tggagatgga agcccgacgc cgccagccgg
     4021 agctcgagga atcgcagcgc cgccgcctgc accgtttcgc cgagacgcag ctgatgaacc
     4081 agctcaccac ggtcgagatc aacaacaaaa tcaaggtgga ggatgccgag gtccgggcgg
     4141 tctggaaccg catggaccgc gaattccacg cccgccacat cctggtgccc acgcgcgacc
     4201 aggccctcga catcaagaag cagctcgacg gaggcgccgc gtgggaggcc atggcggccg
     4261 aacactccaa ggatcccgac aacgcccagc gcgggggcga cctgggttgg gtggccgcgg
     4321 gcgtcatggt gcagccgttc gaggaggcgc tcttctccct ccaggccggc cagatctccg
     4381 atcccatcca gacccgtttc ggctaccaca tcatcaaggt ggatgaggtg cgcaacgtcg
     4441 agcgccccga attcgagtcc gtgcgcgagc gcgtctactc gctgctgctc cagcagcgct
     4501 cgctggcccg gcaggaagaa ttccagaaga tgctcaacga gaaggccaag ccggccaacc
     4561 agctcgatgc catccagctc atcgacaaga agtattactt cgaggtgccg gcggcccagg
     4621 ccaacgaccc gtacgcgctt ctcaacgcca accggatgac gccggccttc tcgaaggaag
     4681 agctggccat gccggtcatc aagttcgccg atcgtcccga cttcaccatc caggacttca
     4741 acgaggccct gtcgtggatg ccccccggcc tctggcccag cggcaagggg ctcgaggaaa
     4801 tcgagacgct tctccggcag atgacccgca ccaagctcta ccgcgaccac gccatctcgc
     4861 tcggcatcga gcagaccccc gagtatgtcc gcacgatcca gaagaaggaa agcgagatgc
     4921 gggtgaacgg gctctactac aacggcatcg tggggagcat caccatcacc gataccgaca
     4981 agcgcgagtt cttcgacgcc caccgggaca actacaagat cgccgatcgc taccgcctct
     5041 cgcgcatcga gacgcccgac tcggccgtgg cccagctggc ctggcggaac tggaagaccg
     5101 gcaaggattt cgaggacgtg gcgtcgatgg tcaagggact cgatcccacg gccggcgcca
     5161 cggcggccac cttcgagatt ccgaaggggc agtcgccgcc ggtcgacgac ctggtcttca
     5221 aggggcaggt cggcgacatc atcggccccg tgcacctgga aagctcggtg ctggagtcgg
     5281 gccagacgct gccgccccgg tggctggtgg ccaagatcct cgacatcaag ccggaacggc
     5341 agatgaccta cgaggaaggg gagtcgttcg tggccgacca cgccaagacg gcccgggccg
     5401 aggaggagct gaagaagatc ctcgacaccg ccaaggtcac cttccaggtc aagatcgacg
     5461 aggccgcgat ggccagggtc acggccccca tgatccagga agcgccgaat ccgaagaagg
     5521 acgcggccgc caacagctga gctcggcgcc cgtttcggcg ccggcgcgcg tttcggcgcc
     5581 ggcgaccgtc agtacgatat cgcgcggagg agcgtcacct gccagtggtg agccaggagc
     5641 gccttgagca gggcgtcgag gatcaccagc gccagtgaca gcgaaagata gtgagacgcc
     5701 ggcccgggcc accgggccgg cttgtctttc aggcgccgga tcacggtggt gaagagccag
     5761 gccatggcca cgccgccggc gatgaatccc accacgcgga tgatcgacca gatcggccat
     5821 gccatgagcg acagccgcag tccgtcgttg gcgcccgcat cgttcaggaa gagcgacccg
     5881 acgtagaagc tcatgtagtg cacgaggacc gcgcccagca cgagcccggc cacgccgcac
     5941 gtgagcgccg acag
//
LOCUS       DASVOD010000754         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_571560, whole genome shotgun sequence.
ACCESSION   DASVOD010000754 DASVOD010000000
VERSION     DASVOD010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571560"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VF720_16155"
     CDS             <1..1048
                     /locus_tag="VF720_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880946.1"
                     /translation="RPPERWFSQLDPGAASVTRMDRLALAGIRGGSLLGGRRLPTAEW
                     APPTSPDAPLDWARGRLAGGGLADGRSAEGGSVVLRTFASSAVRLAERARARDLDLTG
                     LVIFSGGEPLTDARRRYIESTGARVFPRFVVTEAGLLAAACPHRVTTDSMHVYVDRLA
                     MIEGDPGDAGDAGRAGEAGGAPTPLLLTTLTPDTGKVLFNTEMGDAGLLTRRGCPCAF
                     GRLGFDLFVSQVSSRERLTLEGMTVPVRAVDASIAAVVESLGGSPDAWQRRVVVEESG
                     SARLVVVIHPDLGPLDEAVLLADVHARLATAGAGLDVAADLWRQAGTIRVVREAPARS
                     PGAKLPRGVTGARS"
     gene            1045..1806
                     /locus_tag="VF720_16160"
     CDS             1045..1806
                     /locus_tag="VF720_16160"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7880947.1"
                     /translation="MNRDPRGPSGDFDPDGWDRMARGQAGGAAWIFERAIERVAELAG
                     SPSPGGSRRLLDVGCGTGRLATKLVRAGWEVTALDRDSLMVRRACAWDASSVDVGFRS
                     VVTADATALPFRNATFARITASSLLGCLDDADTFLAEAYRCLEPDGALILTATNRDSW
                     LLKLNHALPRAWVVPKSAGPRQKFTTYSFGELRGRLHGAGFRVDPCHAFNFVVHAGPI
                     LFPPRSVARGLDSFGVNGALRRRARNLVAVAWKPA"
     gene            complement(1877..>2567)
                     /locus_tag="VF720_16165"
     CDS             complement(1877..>2567)
                     /locus_tag="VF720_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880948.1"
                     /translation="LREGKRPDLVFTYDGVNDTYAAYQSGVPGTNQNVSDMEAILHDR
                     RSSFEYFRAGLSGLLREHLMIYRAVRGGTDMLRAKMNPRTRFQEAGAGMSAGDLERLS
                     EGIVAEYWRNQDILDRLAASYGFRVLRFWQPSAYAQNWAQAAAVDIRATDTALRDLYR
                     SASARVPDDPARRFTNLSQLFAGREDSVFIDFVHISEEGNAEVAGKMIEALRVSDGAA
                     WCGAVAGEVQE"
BASE COUNT          349 a          906 c          896 g          416 t
ORIGIN      
        1 ccgcccgccc gagcgctggt tctcgcagct cgatcccggt gccgcgtcgg tcacgcgcat
       61 ggacaggctg gctttggccg gcatccgcgg cggcagcctc ctcggcgggc gacggcttcc
      121 caccgccgaa tgggctcctc ccacctcgcc cgacgcgccg ctcgactggg cgcgcggccg
      181 gctggcgggc gggggactcg cggacggacg gtcggctgag ggcggcagcg tggtcctccg
      241 cacattcgcc agctcggccg tccgactggc ggagcgcgct cgcgcgcgcg atctcgacct
      301 cacgggcctc gtcatctttt cgggcggtga acccctcacc gacgctcgcc gccgctacat
      361 cgagtccacc ggcgcccgcg tcttcccgcg cttcgtcgtg accgaagcgg ggctgttggc
      421 cgccgcctgc ccccaccgcg tcacgaccga ctccatgcac gtgtatgtcg accggctggc
      481 catgattgag ggggacccgg gcgatgcggg tgacgcgggg cgagcgggtg aggccggcgg
      541 tgcccccacg ccgctcctcc tcaccacgct cacgcccgac accggcaagg tgctcttcaa
      601 taccgagatg ggcgacgccg gcctgctcac gcggcggggg tgcccgtgcg ccttcgggcg
      661 gctcggcttc gacctgttcg tctcacaggt ttcgagccgt gaacgactca cgctcgaagg
      721 gatgaccgtg ccggtccgcg cggtcgatgc ctccatcgcc gccgtcgtgg agtctctggg
      781 tggatcgccg gatgcctggc agaggcgagt cgtggtcgag gagagcggca gcgcccgcct
      841 cgtcgtggtc atccatcccg acctcggccc cctcgatgag gccgtcctcc tggcggacgt
      901 gcacgcccgt ctcgccaccg caggggcggg cctcgacgtg gccgccgacc tctggcggca
      961 ggcgggcacc atacgcgtcg tccgcgaggc gcccgcgagg agcccgggag cgaagcttcc
     1021 gcggggcgtg acgggggcgc gatcgtgaat cgcgatcccc ggggcccgtc gggcgatttc
     1081 gatcccgacg gctgggaccg gatggcgcgc ggacaggccg gtggcgccgc gtggatcttc
     1141 gagcgcgcca tcgagcgcgt ggcggagctg gcgggctcgc cgtcgccagg tggatcccgg
     1201 cgcctgctcg acgtgggatg cggcacgggg cggctggcca cgaagctggt tcgagcggga
     1261 tgggaggtca cggccctcga tcgagactcg ctcatggtgc gacgcgcatg cgcgtgggat
     1321 gcgagctccg tcgatgtcgg ctttcgatcc gtcgtcaccg ccgatgcgac ggcgctccct
     1381 tttcgcaacg ccaccttcgc ccgaatcacc gccagctcgt tgctggggtg cctggatgat
     1441 gccgatacct tcctggccga agcctaccgc tgcctcgaac cggacggcgc gctcatcctc
     1501 accgccacga accgtgacag ctggctgttg aagctgaacc atgccctgcc gcgcgcgtgg
     1561 gtcgtgccga agtcagccgg accgcgccag aagttcacga cctattcatt cggcgagctg
     1621 cgcggccgcc tgcacggcgc cggctttcgc gtggacccct gtcatgcttt caatttcgtg
     1681 gtacacgcgg gcccgatcct ctttccacca aggtccgtgg cgcgggggct ggactcattt
     1741 ggagtcaatg gtgcgctgcg ccgccgcgcg cgcaaccttg ttgcggtggc atggaagcct
     1801 gcctgagggg cggcaccgcc cttctccggg agagactgtt ccggggatgg cccgaacgat
     1861 catcgcgcgt acgcgactat tcctggacct ctccagccac cgccccgcac cacgccgccc
     1921 catccgacac cctcagcgcc tcgatcattt tcccagccac ctcggcgttc ccctcctccg
     1981 aaatgtgcac gaagtcgatg aatacggagt cctcccggcc ggcgaacagc tgcgagaggt
     2041 tggtgaagcg gcgcgcggga tcgtcgggga cacgcgccga ggccgagcga tagaggtcgc
     2101 ggagggcggt gtcggtggcg cggatgtcga ccgcggcagc ctgggcccag ttctgagcgt
     2161 acgcggaggg ctgccagaag cgcagcaccc tgaaaccgta tgacgccgcc agacggtcga
     2221 ggatgtcctg gttccgccag tactcggcca cgattccttc cgacagccgt tcgaggtcac
     2281 cggccgacat gccggcgccg gcttcctgga agcgggtgcg ggggttcatc ttcgcccgga
     2341 gcatgtcggt gccgccgcgt acggcgcgat agatcatcaa gtgttcacgc aacaggcccg
     2401 agagtcccgc gcgaaagtac tcgaaggagg aacgcctgtc gtggaggatc gcctccatgt
     2461 cggacacgtt ctggttggtg cccggcacgc cggactggta ggccgcatag gtgtcgttga
     2521 cgccgtcgta cgtgaacacg aggtccgggc gcttcccctc gcgcagg
//
LOCUS       DASVOD010000755         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_206919, whole genome shotgun sequence.
ACCESSION   DASVOD010000755 DASVOD010000000
VERSION     DASVOD010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206919"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="VF720_16170"
     CDS             complement(<1..1026)
                     /locus_tag="VF720_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HEX7880949.1"
                     /translation="MKPTLSDAALHEWLTRLSRANADVQRLFPGDRPGRQPVHTVYGG
                     AHLFRFNTAPRLGKTALAILNEYAAMPADLSAALGLDAGMAARVHERIVGKLSHEPVE
                     DFRIDFEDGYGNRSDAEEDGHAVSAAAEVAKGFEAGSLPPFIGIRIKPLTEELKARSL
                     RTLDLFLTALLDGTGGRLPANFVVTLPKITSPEQPAVLADMLARLESELALPSGSLVF
                     ELMIETTQSIVNDAGAIAIRGLIDAAPGRIVAAHFGTYDYTASCSITAAWQTMDHPAC
                     DFARHVMQVSLAGTGIWLSDGATNVLPVAPHKAPEGGTLGAREAAENRRIVRDAWKLH
                     YGHVRRSL"
     gene            complement(1023..2324)
                     /gene="thrC"
                     /locus_tag="VF720_16175"
     CDS             complement(1023..2324)
                     /gene="thrC"
                     /locus_tag="VF720_16175"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013534928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX7880950.1"
                     /translation="MRFTSDRGGRPAVSARSAIRAGLAPDGGLYVPESLPKLARGGND
                     WAPGTLFEAGVALLGPYFDDLDEDALRALLVETWDFDAPVVSLEPGLALLELFHGPTL
                     AFKDFGARFLAGYLATGGEPLTILVATSGDTGGAVAAGFLGAPGITVYLLYPAGRVTP
                     IQEAQMATLGGNIRAIEVSGTFDDCQRLVKGALGDARLAADLRLTTANSINLGRLLPQ
                     MAYYGAAFGPVLGTLPGFDSIASSCTLVVPSGNFGNVTAALYARTMGVPIGPIVAAVN
                     ANDVVPRILGGEAADRSVSIHTLSSAMDVAAPSNLSRLLHLAGGSVERLGTLMTAETI
                     DDVTTGAEIRHVYETTGRIIDPHTAVGLAVARRRRAAGDPAVLVASTAHPAKFPEVIE
                     PLIGRVAPLPARLAEALDRPKYARSLPPDDEAFRHLLRTDT"
     gene            complement(2321..>2565)
                     /locus_tag="VF720_16180"
     CDS             complement(2321..>2565)
                     /locus_tag="VF720_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEX7880951.1"
                     /translation="ARAPLIPGFSAVRVAAEKAGAFGMSISGSGPSVFAVTDTDASAK
                     RVAAAMVRAFRIAGGVGARSWISRINRRGARIERRG"
BASE COUNT          391 a          842 c          952 g          380 t
ORIGIN      
        1 cagcgaacga cggacgtggc cgtagtggag cttccacgcg tcgcgcacga tgcgccgatt
       61 ttcggcggcc tcgcgggcgc cgagcgtgcc accttcgggc gccttgtggg gcgccaccgg
      121 caggacgttg gtggcaccgt ccgagagcca gattcccgtg ccggccagag acacctgcat
      181 gacatgccgg gcgaagtcgc aggcggggtg atccattgtc tgccacgccg cggtgatcga
      241 gcaggaggcg gtgtagtcgt aggtgccgaa atgggcggcc acgatgcggc cgggcgccgc
      301 gtcgatgagg ccgcggatgg cgatggcgcc ggcgtcgttc acgatcgact gggtcgtctc
      361 gatcatcagc tcgaagacga ggctccccga ggggagcgcg agttcgctct cgagccgcgc
      421 cagcatgtcg gccagcacgg ccggctgttc ggggctcgtg atcttgggga gcgtgacgac
      481 gaagttggcc ggcaatcgcc cgccggttcc atcgaggagg gccgtgagga acaggtcgag
      541 cgtgcgcagg ctccgggcct tgagctcctc ggtgagcggc ttgatgcgga tgccgatgaa
      601 cggcgggagg cttcccgcct cgaacccttt tgccacttcg gcggcggcgg agacggcgtg
      661 gccgtcctcc tcggcgtccg atcggtttcc gtacccatcc tcgaagtcga tgcggaagtc
      721 ctcgaccggt tcatgcgaga gttttccgac gatccgctca tggacgcggg cggccatgcc
      781 ggcatcgagg ccgagcgccg ccgacaggtc ggccggcatg gcggcgtact cgttgaggat
      841 ggccagcgcg gttttcccga ggcgcggggc cgtgttgaag cggaagaggt gcgcgcctcc
      901 atataccgta tgcaccggct ggcgcccggg gcgatcgccg gggaagaggc gctgcacgtc
      961 cgcgttggca cgcgacagtc gggtgagcca ttcgtggagc gcggcgtcgg aaagcgtcgg
     1021 tttcatgtgt cggtcctcag gagatgccgg aaggcttcgt cgtcgggtgg cagggatcgc
     1081 gcgtatttcg gccgatccag ggcttcggcc agccgggcgg gaaggggcgc cacacgcccg
     1141 atcaacggtt cgatcacttc gggaaacttg gccgggtggg cggtggaggc caccaggacg
     1201 gctggatcgc cggccgcgcg gcggcgccgg gcaacggcca ggccgacggc cgtgtgcggg
     1261 tcgatgatgc gaccggttgt ctcgtacacg tggcggatct cggcgccggt ggtgacgtca
     1321 tcgatggttt cggcggtcat gagcgtgccg agacgttcca ccgaaccgcc ggccaggtgg
     1381 agcaatcggc tcaggttgct gggcgccgcc acgtccatgg cgctggagag ggtgtggatc
     1441 gacacgctgc gatcagcggc ctcgccgccc aggatacgcg gcaccacgtc gttggcgttc
     1501 accgccgcca cgatcggtcc gatcggcaca cccatggtgc gggcatacag cgcggccgtc
     1561 acgtttccga agttgccgct cggcacgacc agcgtgcagg aactcgcgat gctgtcgaat
     1621 ccgggcagcg tgccgaggac cgggccgaac gcggcgccgt agtacgccat ctgcggaagg
     1681 agccgcccca ggttgatcga gttggccgtg gtgagccgca ggtcggcggc caggcgggca
     1741 tcgccgagcg cgcccttcac gaggcgctgg cagtcgtcga acgtgccgct cacctcgatg
     1801 gcgcggatgt tgccccccag cgtggccatc tgcgcctcct ggatcggcgt gacgcgaccg
     1861 gccgggtaga ggagatacac cgtgatgccg ggcgccccga ggaagccggc cgccaccgcg
     1921 ccgccggtgt cgccactggt ggccacgagg atcgtgagcg gctcgcctcc cgttgccagg
     1981 tagccggcca ggaagcgcgc gccaaaatcc ttgaaggcca gcgtcgggcc gtggaagagc
     2041 tccaggagcg cgaggcccgg ctcgagcgac acgaccggcg cgtcgaaatc ccaggtctca
     2101 acgaggagcg cgcgaagcgc gtcctcatcc aggtcatcga agtacggccc cagaagcgcc
     2161 acgccggcct cgaacaaggt gccgggcgcc caatcgtttc caccgcgtgc cagtttcggc
     2221 agcgactcgg gcacgtagag gccgccgtcc ggagcaagcc cggcgcggat ggcggaacgg
     2281 gcggaaaccg ctggtcggcc gcccctgtcg ctcgtgaacc tcatccccgc cgttcgatgc
     2341 gggcgccgcg gcggttgatg cgcgaaatcc aggagcgcgc gcccacgccg cctgcgatgc
     2401 ggaaggcgcg caccatggcc gcggccaccc gtttggccga tgcgtcggtg tcggttaccg
     2461 cgaaaacgct cggccccgat cccgagatcg acatgccgaa cgcgcccgcc ttttcggcag
     2521 ccacccgcac ggcggaaaaa cccgggatga gcggcgcccg ggccg
//
LOCUS       DASVOD010000756         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_260580, whole genome shotgun sequence.
ACCESSION   DASVOD010000756 DASVOD010000000
VERSION     DASVOD010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260580"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(309..1013)
                     /locus_tag="VF720_16185"
     CDS             complement(309..1013)
                     /locus_tag="VF720_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880952.1"
                     /translation="MKKIVLFALLISASAATPSSALQTLANFRGYDWTWPVNNCLDCP
                     NTYYEAQGFLTSVNPAFITADFTNKEYTVVLGDDLFLADADTFATVIVAHYVNGSIYF
                     YADDKVLGTPASYNVNGDCDPFQDRLRFADGEQILAGNFYNFDVVSTIATGDGSVQGF
                     VNWLGGTQLGNIPLSLRGGWTFGAIGIRAGNTPCGYHWDINGVSTLQQPLPVQPASWG
                     QIKATGGDPGRLVIKR"
     gene            1591..2103
                     /locus_tag="VF720_16190"
     CDS             1591..2103
                     /locus_tag="VF720_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880953.1"
                     /translation="MFPAPLTSRPAVAVIGAIVAAILCTLGPTGAAAQIPGGAIFDSL
                     VTTPVSPPEKVRIDYLFLAFDGSVRSQPVGRTRMEADSLARVLLVVARSGASFDSLKK
                     AHSDEPRLEAMTLVNKGLRPAAGERTRKMVEIAVGDMAFGMSPGDIGLVVPNNTTCRY
                     GYYVVRRLAE"
     gene            2194..2829
                     /locus_tag="VF720_16195"
     CDS             2194..2829
                     /locus_tag="VF720_16195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX7880954.1"
                     /translation="MSRLPRSNATRTTRLAAATLATALLAALTLSARLEATEPTGSTD
                     APAKAATTTTPAPTAKATQGKSGKTNPGKTAMKGTMPVKPATTPEHVQVQHILIGFAK
                     DPNKQSTVPGKPITRTMAEAKALAYKILDQARAGTPFDTLVVQYTEDSPPGIYSMSGI
                     GVTKNPGEWARNEMVAAFGDVGFNISVGNIDIAEYDPAKSPFGYHIIKRLK"
     gene            complement(2926..>3909)
                     /locus_tag="VF720_16200"
     CDS             complement(2926..>3909)
                     /locus_tag="VF720_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880955.1"
                     /translation="NLGSNWLHWDNLAGVPTPLVNELIPSEWLEPGSIDSTHLGDGQV
                     LARHLAPGTIGAMQIAPGSVTTVEVADASLTGDDVANSTLSGDDILNGTITGGDVQDG
                     SLSGADILNGSLTGGDIAAGAVGNTQIQDNSVTESKLFDETITSIKLAPGSVTAEKMA
                     AGSVGGAALQPASITGNHVVDGSLHDSAMIDSPGLDNLSIEGFADSVVSGSGQTIGFL
                     TVDAPGPGLIQVNGTVQVYLLHDSGQASRVTLALSNLEDDVADATSSLVSLPGALPSE
                     LYSFPVSVQALFPVASAGPVTVYLTAKKGSQPRPPSLHNVRVTALFVRRGY"
BASE COUNT          713 a         1292 c         1258 g          646 t
ORIGIN      
        1 ggcggcggag gtcggcggat ttgaccatga cgatcctgtt cggaggtcgg aagttgacgt
       61 cggcggtcga tttcggaagc ggttgagtat aaggaaacgg ccggcgtggg accaaccccg
      121 cgccggccgt tgtggtgatg actgccgtac cttccgggca accggcccga cgtgcctctg
      181 tcatgacgcg tcgagaccgg gtggcgggac cgcaaccgaa ttctccgcca cccgaatccc
      241 gacgtcgaac ccgcggagat cgggcatacc ggtcgagccg gaccggtctg cccgccttct
      301 gcgggacact atcgcttgat caccaaccgg ccgggatcgc ccccggtggc cttgatctgt
      361 ccccagctgg cgggctgcac cggcaggggc tgctggagcg tggagacgcc gttgatgtcc
      421 cagtgataac cgcagggcgt gtttccggcc cggatgccga tggcgccgaa cgtccagccc
      481 ccccgcaggc tgagcgggat gttcccgagt tgcgtgccgc cgagccagtt cacgaatccc
      541 tggacgctgc cgtcgccggt ggcgatcgtc gagaccacgt cgaaattgta gaaatttccc
      601 gccaggatct gttcgccgtc ggcgaagcgg aggcggtcct ggaacgggtc gcaatcgccg
      661 ttcacgttgt agctggcagg cgtgccgagg accttgtcat cggcatagaa atagatgctg
      721 ccgttgacgt aatgggccac gatcaccgtc gcgaaggtat cggcgtcggc caggaagaga
      781 tcgtcgccga gcacgacggt gtactccttg ttcgtgaaat cggcggtgat gaacgcgggg
      841 ttcaccgacg tgaggaatcc ctgcgcctcg tagtaggtgt tgggacaatc caggcagttg
      901 ttcaccggcc aggtccagtc gtagccgcgg aaattggcca gggtctggag cgcgctggat
      961 ggcgtggcgg cggatgccga gatcaggagg gcaaacagaa cgatcttctt catcgaaacc
     1021 tcccagtttg agtggcaacg ggctgtcgtg gccaatgaag caccacggtc gcgattgccg
     1081 aacctcttgc gaggttagcg gccggcaagt gctgggcgat ctggctatca ggtccttgtg
     1141 ggtcggcgta tccgtgactt cggcgatcct ccttccgggc aatcgcgatc ggcccgggcc
     1201 cgggccggga gtcggcaggg acaggcaggc gcgtggcaga atcgggcgag cgatcgcgca
     1261 tcgccgccga tgcggcggat gccggaacgc acgatcccga ggatgatgtc ggagttgaaa
     1321 ccgtcatcga gcgctcatcc ggggggcagt acaggagagc gtcagggacg acggatacga
     1381 tgcagtcgca ggagtaacgc gacttcttac ggaatcgtgc aatactcaag cgtttttttc
     1441 caagcaattc ggacaaattt gaaggcgagt gtcggaatgc tcacgagaag ccttgattgt
     1501 cgtcaagcat gtcgcgcgga tccgggcaaa gcgagcatca ctctcatgtc gtcacacgct
     1561 atactgcgcc ggatgagaaa cccgattcga atgttcccgg cacctctcac ttcccggcct
     1621 gccgttgccg tcatcggggc gatcgtggcg gcgatcctct gcaccctcgg cccaaccggg
     1681 gccgccgccc agatccccgg cggcgccatc ttcgattcac tcgtgacgac gcccgtttct
     1741 cctcccgaga aagtgcgaat tgattacctg ttcctggcat tcgacgggag cgtccgcagc
     1801 cagccggtcg gccgcacccg gatggaagcc gacagcctcg cccgggtgct gctggtggtg
     1861 gcccgctccg gcgcaagctt cgacagcctg aagaaggccc actccgacga accgcgcctc
     1921 gaggcgatga ccctggtcaa caagggtctc cgccccgccg ccggcgaacg gacccggaag
     1981 atggtggaga ttgccgtcgg cgacatggca ttcggcatga gcccgggcga catcgggctg
     2041 gtcgtcccga acaacacgac ctgcaggtac ggatactatg tcgtgcggcg attggccgag
     2101 tgagcgggcc tcatgacgtc ttcccacacg cgaacaggat cgtgtttcaa tttcagacca
     2161 accccgtcaa cagctgacaa ggagagatac gaaatgtccc gactcccccg ttcgaacgcc
     2221 accaggacga cccgtctcgc ggcggccacg ctggccaccg ccctgctggc ggccctgacc
     2281 ctctcagccc gcctcgaggc caccgaaccg accggctcga ccgacgcgcc ggccaaggcc
     2341 gccacgacga caactcccgc accgaccgcc aaggccaccc agggaaaatc cgggaagacg
     2401 aaccccggca agaccgccat gaaaggaacc atgcccgtga agcccgccac cacgcccgag
     2461 cacgtccagg tccagcacat tctcatcgga ttcgccaagg acccgaacaa gcagagcacg
     2521 gtccccggca agccgatcac ccgcacgatg gccgaggcca aggcgctggc ttacaagatc
     2581 ctcgatcagg cccgcgcggg cacgccgttc gacaccctgg tggtccagta cacggaagac
     2641 tcccctccgg gcatctactc gatgtcgggg atcggcgtca ccaagaatcc cggcgagtgg
     2701 gcacggaacg aaatggtggc ggccttcggc gatgtcggat tcaacatctc ggtcggcaac
     2761 atcgacatcg ccgagtacga cccggcgaag agtccgttcg gctatcacat catcaagcgg
     2821 ttgaagtagg tttcgaatcc gggcgccgcg gcgagagaat cgccgcggtg gcccggcgcg
     2881 ttccgggggc acgcggcgtg cggtgaaccc cccgctgtgc cccgctcagt acccccgccg
     2941 cacgaagagc gccgtcaccc gcacgttgtg caggctcggc gggcgcggct ggctcccctt
     3001 cttcgccgtg agatacaccg tgaccggacc ggccgacgcc acgggaaaga gcgcctggac
     3061 gctgaccgga aacgagtaga gctccgatgg gagcgcaccc ggcaggctca ccaggctcga
     3121 cgtggcgtcc gcgacatcgt cttccaggtt cgacaacgcc agcgtcaccc gcgaggcctg
     3181 ccccgagtcg tgcagcaggt acacctgcac ggtgccgttc acctggatca gcccgggccc
     3241 cggggcatcg acggtgagga aaccgatcgt ctgcccgctc cccgagacga ccgagtcggc
     3301 gaatccctcg atcgaaagat tgtcgagccc cggcgagtcg atcatggcgc tgtcgtgcag
     3361 tgaaccgtca accacatggt tgccggtgat cgacgcgggc tgaagcgccg cgcctcccac
     3421 cgatcccgcc gccatcttct ccgcggtcac ggagccgggg gccagcttga ttgaggtgat
     3481 cgtctcgtcg aacagcttgc tctccgtcac ggagttgtcc tggatctggg tgttccccac
     3541 cgcgccggcc gcgatgtccc cgccggtgag gctgccgttg aggatgtccg ccccgctcaa
     3601 tgatccgtcc tgcacgtcgc cgccggtgat cgttccgttc aggatgtcgt cgccggagag
     3661 cgtcgaattg gccacgtcgt cgccggtcag cgaggcgtcc gccacttcca ccgtcgtgac
     3721 ggaacccggc gcgatctgca tggcaccgat cgtccccggc gccaggtggc gggccaggac
     3781 ctgcccgtcg ccaagatgcg tcgaatcgat gctccccggc tccaaccact ccgacggaat
     3841 gagctcgttg acgagtggcg tcggcactcc cgcaaggttg tcccagtgaa gccaattcga
     3901 tcccaggtt
//
LOCUS       DASVOD010000757         9626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_675136, whole genome shotgun sequence.
ACCESSION   DASVOD010000757 DASVOD010000000
VERSION     DASVOD010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675136"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VF720_16205"
     CDS             <1..785
                     /locus_tag="VF720_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880956.1"
                     /translation="WGLGPVVPDQSEYPMDYLLIRLHSTLYKRVEGPIYVGLGFHYDS
                     YDNIVDHRAEQGESTPFTEYSGGSPSTTSSGGFSLNFLGDTRDNLVNPRKGYLLSGSF
                     RDYSRSLGADDGWQELLLDVRLYSNIPQTRRNTLAFWGSGWMTFGPGPYLSLPASGWD
                     TYGRGARGYLQGRIRGESQLYIEGEYRMELTKDGLIGAVAFVNSTFTTEPETRLFSRG
                     DLGAGGGLRMKFNKKSDTNLVLDYGWGREGQRGLVMAMSEAF"
     gene            807..1784
                     /locus_tag="VF720_16210"
     CDS             807..1784
                     /locus_tag="VF720_16210"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX7880957.1"
                     /translation="MKKRQLGPLNVSALGLGCMAMSAFYGSHDDDESLKVIDRALELG
                     IDFLDTADVYGPWTNEELLGRALRGRRERVILATKFGNEINGAGQMVGRINGRPDYIR
                     QAVDGSLKRLGTEYIDLYYQHRVDADTPIEETVGAMARLVAEGKVRFLGLSEAGPATI
                     RRAAAVHPIAALQTEYSLWTRDPEDGILDTVRELGIGFVVYSPLGRGFLTGKFASPDD
                     LEEDDWRRRHPRFEPENFNANRSIVTSIREMASQKGCTAAQLALAWVLSRGNDIVPIP
                     GTRHINYLEDNIAALDLNLTESELSHLDEAVPKGATSGTRYRDMSSIGR"
     gene            complement(2150..2467)
                     /locus_tag="VF720_16215"
                     /pseudo
     CDS             complement(2150..2467)
                     /locus_tag="VF720_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
     gene            complement(2837..3550)
                     /locus_tag="VF720_16220"
     CDS             complement(2837..3550)
                     /locus_tag="VF720_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880958.1"
                     /translation="MTTWRKCFAIIGLLGLTAAALPSRSEAAGNYNAKIILHALSPTT
                     KNQCVRAANFPASCLGYNGGVNNLGLYPTSYHTYLLVVDGSQTEGIAGVQCGISYNPA
                     PGEGVDVYTWNLCATLEFSSTGWPASGGGNLITWDATTRCQTSGNALTGAVAVAGYFY
                     MAAYTPDLMKVTPRPVDGLGKVANCGSVEDIINSCPLNDGFFYGAAGFGRSGHNPCGF
                     SCPLPVEAKTWSGVKVLSR"
     gene            3830..5035
                     /locus_tag="VF720_16225"
     CDS             3830..5035
                     /locus_tag="VF720_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEX7880959.1"
                     /translation="MSILRAAFPVGLLILALPAHAADPCTNANDFPAWMAAFKQEAAQ
                     SGISQKTIDTALKGVTMDPATISRDRKQSFYWQDFMTFSTRLATQDRLTRGRKKLATE
                     SKLFARIESEFGVPGPVIAAFWALESDFGLAQGKMPILRSLATLAWDCRRGPMFREQL
                     LDALRVVDRGDLRPAEMVGSWAGELGQMQFLPSHYLDHGVDYDGNGKVNLLTSTPDVT
                     ASTAKYLQHIGWRRGEPWLEEVRAPREMDWREADLSIRKSRSTWVKLGVTRPDGTALP
                     AGDIEAALLLPMGRFGPAFLAYPNFDAYLEWNESLNYAITAAYLARRIAGDPPMRKGN
                     GTPPVFDLAGIERLQRLLAGAGYEVGKVDGKLGAATRAAVRQAQIRFQLPADAYPSHD
                     LLTRLSAGR"
     gene            5095..7416
                     /locus_tag="VF720_16230"
     CDS             5095..7416
                     /locus_tag="VF720_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880960.1"
                     /translation="MKLRGRSLSWLLLLFLAACGEDSPAPPVDSEAPVIQAVSPGPWA
                     RVSGTTSLSIQANGARRFAYSVDDGTPVESATSPIAWNSADVLNGPHGLRLIASNGAG
                     DVDTTIFLVCDNPGRGTAVLISPREVTVEPNAEYQFEAMLLGGVPGGVTWSLVAPGDG
                     VDWGQIDANGMYTAPNGLPSPPTVTVLARSVDDPGKASTARITIAGVAVRILGAPASL
                     EPGQSVQLGSQVQGLEDQAVTWSLEESPAHGTMTATGRYTAPFSRPDPPIARIRATSV
                     AQPGKSSTVTISLLPAPPPPPPTTQVTVSPSTAQVRSGKTQNFTAAITGISDQRVTWK
                     VDGGSNFGTVSTTGQYRAPSSVPPGGLAVIRATSYADPTQSGTANIQVRPAVSLSVSP
                     PVSDVMPAAIVNFIANVSGADDPRVTWSLSGPSPRGLVSEAGVYTAPEVVSEAHEVTL
                     TVRSVEDPTVYDTATIRLKQSQQLAELTRLGSIGYEFAEISDQALGAVMSILQLARSL
                     NGGQTPTTGRIVHNASGYHYEAIPEATNLEVVGLARTIRLTTEDLTGVPATADQPWPM
                     TVNLFEGSFSGRLEIDGLLNLRVVHSSERDDSWKIHFSRTVRGDLHVSDYTGVGMTHE
                     GVIHAFQSHYYRTEHHIVGNAVMLGGVTYDVDEDMDLTSWEVCYASPYRLGEGWHHRH
                     RNSVTFNGLTRTVDGRVEIDYFQNCGLGSPKQVQGQVITGSLLRNGESLGNLTVTGED
                     GTEVGIALESGTVLFRNSGAVPQRTTFISHPVP"
     gene            7532..8587
                     /locus_tag="VF720_16235"
     CDS             7532..8587
                     /locus_tag="VF720_16235"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX7880961.1"
                     /translation="MPRPTFAAPPPSPAVIDAPSHITIEDLAPGVVAVTRNEPLGLAN
                     HANSIFIIGDSSVVVVDAQFTWAATEEVVAALKARTDKPVRYLVNTHWHDDHLFGNTV
                     WRRHYPGVVIVSHEKTREDLSSIGAENRKGQLEGGPEAVTMFEDAMKNKVTMEGTPMS
                     PAELDAYRSTVDIARQYIAEAPGNDPPLPDLVFRDRMTIYQGNRAIELRYFGEGITRG
                     DAVVWLPAEKIAVTGDLFNVPFPFAYGCHPTAWIAALSGIRELGPAILVPGHGPVQRD
                     LIALNRQAAALAAIREAAVTAVANGVTEEAFRQSVTLEDYRQPMTGENKMLGFLFRSY
                     FAGPAAAAAFREVSAAR"
     gene            8616..>9626
                     /locus_tag="VF720_16240"
     CDS             8616..>9626
                     /locus_tag="VF720_16240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880962.1"
                     /translation="MIPTLLRVRRQRLVNLIRYHFDRGTILWVGLTIALVVVWQWPHL
                     HLPTIWRDRTARGAPDRWFFGALGWLPVLAVTFYALARGVKARSAEYFWLHLVGAPKS
                     LTRRWMILRQLMRTLPIILAVAIPTMLPGGAPGGWWHGPVLIVGYTVVALIALMAVHL
                     GPEDETRMERKERERKTRDQVESFRIVPRGPLSALLVRDLAYLQRGAAGSLGMCGAAA
                     IGEFFVAQSALSTPSGTLAILIVHAIAALLTVNGLLALFEHDAAHPGLLRTLPIPGRR
                     FWAARVLIAMLLCAVPIVPAFVLAVLVPLPLVELVAIAGLGFVVIPAVAGILYANIGV
                     ALF"
BASE COUNT         1652 a         3251 c         3082 g         1641 t
ORIGIN      
        1 cgtggggcct cggcccggtg gtgcccgacc agtcggaata tccgatggat tacctcctga
       61 tccgcctcca cagcaccctg tacaagcgcg tcgaaggccc gatctacgta gggctgggct
      121 tccactacga ttcgtacgac aacatcgtcg accaccgcgc cgaacagggc gaaagcacgc
      181 cgttcaccga atacagcggc ggctccccat ccaccaccag ctccggcggc ttctcgctca
      241 acttcctcgg cgacacgcgc gacaatctcg tgaatccccg caagggatat ctcctcagcg
      301 gaagcttccg cgattactca cgaagcctcg gagccgacga cggctggcag gaactcctcc
      361 tcgacgtccg tctctactcc aacatcccgc agaccaggcg caataccctg gccttctggg
      421 gatcgggctg gatgacgttc ggcccgggcc cctacctcag cctccccgct tccggctggg
      481 acacctacgg ccgcggcgcc cgcggctacc tgcaggggcg catccgcggc gaaagccagc
      541 tctacatcga gggtgaatac cggatggagc tcacgaagga cgggctcatc ggcgccgtgg
      601 cgttcgtgaa ctcgacattc accaccgagc cggagacgcg cctcttctcg cgcggcgacc
      661 tcggcgcggg cggcgggctc cggatgaagt tcaacaagaa atcggatacg aacctggtac
      721 tggactacgg ctgggggcgc gaagggcagc gggggctggt gatggcgatg tcggaggcgt
      781 tctaggtcaa cgaaagcggg aattccatga agaaacgcca actgggacca ctcaacgtat
      841 ccgccctcgg cctcggctgc atggcgatga gtgccttcta cggctcgcac gatgacgacg
      901 agtcgctcaa ggtgatcgac cgcgcgctgg agctgggtat cgacttcctc gacacggccg
      961 acgtgtacgg cccatggacc aacgaggagc ttctcggcag ggcacttcgc gggcggcggg
     1021 agcgggtcat ccttgccacc aagttcggca acgagatcaa cggggcgggg cagatggtcg
     1081 gccggatcaa cggacggccc gattatatcc gccaggccgt cgatgggagc ctgaagaggc
     1141 tcggcaccga atatatcgat ctctactacc agcaccgcgt ggatgccgac acgccgatcg
     1201 aggagaccgt cggcgccatg gccaggctcg tcgccgaagg gaaggtccgc tttctcggcc
     1261 tgagcgaggc cggtcccgcc acgatccgcc gggcagcggc cgttcacccc atcgccgcgc
     1321 tccagaccga gtactcactc tggacccgcg atcccgagga cgggatcctc gacacggtcc
     1381 gcgagctcgg catcggcttc gtggtctaca gcccgctcgg gcgaggattc ctcaccggca
     1441 agttcgcctc acccgatgac ctcgaagagg acgactggcg gcgccgccat ccccggttcg
     1501 agcctgagaa cttcaacgcg aacaggtcga tcgtcaccag catccgggag atggccagcc
     1561 agaagggttg caccgccgcc cagctcgcac tcgcctgggt cctctcccgc ggcaatgaca
     1621 tcgtccccat tccgggaacc cgccacatca actatctcga ggacaacatc gccgccctcg
     1681 acctgaacct gaccgagtcc gaactgagtc acctcgacga ggcggtgccg aaaggggcca
     1741 catccgggac gcggtaccgc gacatgtcga gtatcgggag gtaagacgcg gcacggcgcc
     1801 cttctcctca ggtctgcgcc cgctccgccg tcatccggaa ttcgccctcg ctgatcgtca
     1861 gccgcttggc cggccccgca cccgcttcga acgaaatccg cacgcgatca ttccccggcg
     1921 gatggaaggc cagcgatccc tggtgataca ggttccggaa cccgcccgcc ggcagccgct
     1981 tcaggccaag cgcgccatcc ttgttcaacg acacgtcgaa acgctcgtcc ggcgcaccac
     2041 cgaacgcata cacgccaacg agccgatcgc gctccgcgtc ggcaagcggt tcgttccggt
     2101 agaccggatc cgcatcaccc agcgactcga ggtactgcac gaccggcagc gaagcctcac
     2161 caccctttcg ggcatgatgc agcagcgtca gcccgtgcgg cccgtgcacc cgctggatgc
     2221 cgggatacgc cacgaccgcg gcgcgcaccg catcgacatg cccgagcatc gtgagcgtga
     2281 agaggtccgg acgcgcgccg ttctcgacca gcaacgccac gatctcccgg ttccccacgt
     2341 ggctcgcggc gccgatggcg gtttcccagt cgccgaagcc ccaatcccac gtagccctgg
     2401 caaacgtggg gctcacggcc aggagctccc gcacgcgggc gatgttgccg tgggaggccc
     2461 cgaccatgtc gcgcacaagg gttgggtcct gggccggaaa gttcgcggat ggagtggctg
     2521 ggccgctggc tggctggggt gggtcggcgg ccttggcagc cttggcgttc aggccaacgc
     2581 ggaccgccaa ggccccgact gagaggaaga ggaattcgcg gcggtggagt gacatgggag
     2641 cctccgatga atgtcgacgg aagtgcggtc gagacataga cgcatgcacg accctccctg
     2701 cttgaaaccc tattcgacct gggagcctgt ccaggcattg atcaaggagc tggcccgtgt
     2761 tgagttgttg cggttggttt tcaacaggtc ttttcgattg attgggccgg tttcccggac
     2821 atactcggac aggctcctag cgggagagca ccttcacgcc cgaccaggtc ttcgcttcca
     2881 cgggaagtgg acaggaaaac ccgcatggat tgtgcccgga gcgaccgaag cccgcggcgc
     2941 cgtagaagaa gccgtcgttg agagggcagc tgttgatgat gtcttcgacg gagccgcaat
     3001 tggcgacctt gccaagcccg tcaaccggcc gcggcgtaac tttcatgagg tcgggcgtat
     3061 acgcggccat atagaagtat ccggccacgg caaccgcccc cgtgagtgcg ttcccggagg
     3121 tctggcagcg agtggtggca tcccacgtga tcaggttgcc gccgccggac gcaggccatc
     3181 ccgtcgaaga gaattcgagc gtggcgcaga ggttccaggt gtagacatcc actccctcac
     3241 cgggtgccgg gttatagctg atgccgcact ggacgccggc aattccctcc gtctggctcc
     3301 cgtcaaccac cagcagatag gtgtgatagc tggttggata cagcccgaga ttgttgaccc
     3361 ccccgttgta tccgaggcaa ctggccggga aattggccgc ccggacacac tggttcttgg
     3421 tcgtcggtga gagcgcgtga aggatgatct tggcattgta gttgcccgcc gcctcgctcc
     3481 tggaaggcag ggcggctgcc gtgagtccga gcaaaccgat gatcgcgaag cacttgcgcc
     3541 aagttgtcat cgtggcctcc tgggatgccg tgcaaatggg cgctcgattc ggacgcgcct
     3601 tttccggaaa ggtgagcgcg gcctcgacca aaatacgatc attcgccggg tccatggccc
     3661 cgcctcgacg agaatactcg gacaggctcc tcggtccgac tcgtggcaac tgacgggttt
     3721 gaaggcacat ccgtcgacaa ggagcatacg cgccagaatc agtttctggt gttacttctt
     3781 gccatctgct gcgcgcatga gccactctcg cctgctagac tcgtttgcca tgtctatcct
     3841 acgagccgcc ttccctgtcg gtctgctcat acttgccctt cccgcccacg ccgccgaccc
     3901 ctgcaccaac gccaacgact tccccgcctg gatggccgcc ttcaagcagg aagccgccca
     3961 gtcgggcatc tcccagaaga cgatcgacac cgccctgaag ggcgtcacga tggaccccgc
     4021 cacgatctcc cgcgatcgca agcagagctt ttattggcag gacttcatga cgttttcgac
     4081 gcggctggcc acgcaggatc gccttacgcg cgggcggaag aagttggcga cggaatcgaa
     4141 actcttcgcg cggatcgagt cggagtttgg tgttccgggg ccggtcatcg ccgccttctg
     4201 ggcactcgaa agcgacttcg gactcgcgca gggaaagatg ccgatcctgc gatcgttggc
     4261 cacgctggcc tgggattgtc gccgcggacc gatgttccgc gagcagttgc tcgatgccct
     4321 gcgtgtcgtg gatcgcggtg acttgaggcc ggccgaaatg gtcggctcct gggcgggtga
     4381 attgggacag atgcaattcc tgccgtcgca ctacctggac catggcgtcg attacgacgg
     4441 gaacggaaag gtcaatctct tgacctccac gcccgatgtc acggcatcga cggccaagta
     4501 cctccagcat atcggatggc gacggggcga gccatggttg gaagaggtgc gcgcacccag
     4561 ggagatggac tggagggaag cggacctgtc gatccggaag tcccggtcaa cgtgggtcaa
     4621 gctgggggtg acgcgtccgg acggcacggc gctgccggcg ggagatatcg aggcggcgct
     4681 tctcttgcca atgggtcgat tcggtccggc gttcctggcc tatcccaact tcgatgccta
     4741 cctggaatgg aacgagtcgc tcaattacgc gatcacggcc gcgtacctcg cccggcgcat
     4801 cgcgggcgat ccccccatgc gaaaagggaa tggcacgccg ccagtcttcg acctggcggg
     4861 catcgagcgg ctccagcgcc tcctggcggg ggcgggatac gaggtcggga aggtcgatgg
     4921 gaagctggga gcggcgacga gggcggcggt ccggcaggcc cagatccgtt tccagttgcc
     4981 ggcggacgct tatccctcac atgacctgct gacgcgcctg tcggccgggc gatgaccgac
     5041 tgatattcat tggtacgggg gccattcatg gccccctcat ctatacttcc atttgtgaaa
     5101 ctccgtggcc gaagcctctc ctggctgcta ttgctgtttc tcgccgcctg cggcgaggac
     5161 tcccccgcgc cccctgtcga ttccgaggcg cctgtcatcc aggcggtgtc gccgggaccg
     5221 tgggcgcgcg tatcgggaac aacctccctt tcgatccagg caaacggcgc acggcgattc
     5281 gcttattccg tcgatgacgg cacgccggtc gaatccgcca cctcgccgat cgcctggaat
     5341 tccgccgatg tcctgaacgg cccccacggc ctccggctca tcgcgtcgaa cggggcgggc
     5401 gatgtcgaca ccactatctt cctggtgtgc gacaaccccg gtcgtggcac ggcggtcctg
     5461 atctcgccgc gcgaggtcac ggtcgagccg aatgcggaat accagttcga ggcgatgctc
     5521 ctcggcggtg tcccgggcgg cgtcacgtgg tcgctggtcg cgcccggtga tggggtcgac
     5581 tgggggcaga tcgacgccaa cgggatgtac accgccccga acgggttgcc ctcgcctccc
     5641 acggtcaccg tgctggcccg cagcgtggac gaccccggca aggcctcgac ggcccggatc
     5701 acgatcgcgg gcgttgccgt gcgcatcctc ggggcgccgg cttcactgga gccgggacag
     5761 tcggtccagc tggggagcca ggtacagggg ctggaagacc aggccgtcac ctggagcctc
     5821 gaggaatcgc ccgcccacgg caccatgacc gccacggggc gatacacggc accgttcagc
     5881 cgccccgatc ccccgatcgc gcggatccgc gccacgagcg tggcccagcc cggcaagtcg
     5941 tcgaccgtga cgatctcgct cctccccgcg ccgccgccac caccaccgac gacgcaggtc
     6001 accgtctctc cctccacggc ccaggtccgg agcggcaaga cgcagaactt caccgccgcc
     6061 atcaccggaa tttccgacca gcgggtcacc tggaaggtgg acggcgggag caatttcggc
     6121 acggtttcga ccacgggtca gtaccgggcg ccctcgtcgg tgccgcccgg cgggttggcg
     6181 gtgattcggg ccaccagcta cgccgatccg acgcaatcgg gcaccgccaa catccaggtg
     6241 cggccggccg tcagcctgag cgtgtcgccc ccggtgtccg acgtgatgcc cgccgccatc
     6301 gtcaatttca tcgccaacgt cagcggtgcc gacgatccgc gcgtcacctg gtcgctctcc
     6361 gggccatcgc ctcgcggact cgtgagcgag gcgggcgtgt acaccgcgcc ggaggttgtt
     6421 tccgaagcgc acgaagtcac gcttaccgtc cgcagtgtgg aagacccgac ggtctacgac
     6481 accgccacga tccgcctcaa gcagagccag caactggccg aactgacgcg gctcggttcg
     6541 atcggctacg aattcgccga gatcagcgac caggccctcg gggcggtgat gtcaatcttg
     6601 cagctggcgc gcagcctgaa cggcggccag acccccacca cgggacgcat cgttcacaac
     6661 gcaagcgggt atcactacga agccatcccc gaggccacga acctcgaagt cgtgggattg
     6721 gcgcggacca tccggctcac gaccgaagat ctcaccggag ttccggccac ggccgatcaa
     6781 ccctggccga tgacggtgaa cctcttcgaa ggaagcttca gcggccggct cgagatcgac
     6841 ggcctgctca acctgcgcgt ggtccacagc agcgagcgtg acgacagctg gaagatccac
     6901 ttctcccgca ccgtgcgggg cgatctccat gtctccgact acacgggtgt cgggatgacg
     6961 cacgagggag tcatccacgc cttccagtcg cattactacc gcacggagca tcacattgtc
     7021 ggcaacgcgg tgatgctggg cggcgtcacg tatgacgtcg acgaggacat ggacctcacc
     7081 agctgggagg tttgctacgc cagcccgtac cggctgggcg aggggtggca ccatcgccac
     7141 cgcaacagcg tcaccttcaa cggcctcacg cggacggtcg acgggcgtgt cgaaatcgac
     7201 tacttccaga attgcgggct cggcagcccg aagcaggtac aaggacaggt aatcacggga
     7261 agcctcctgc ggaacggcga atcgctcggc aatctcaccg tgacgggcga ggatggcacc
     7321 gaggtcggca tcgcgctcga atcaggcacc gtgctgttcc gcaacagcgg ggccgtaccc
     7381 cagcggacga cgttcatttc gcatcccgta ccgtgagcag gtggttgagg cctcgctcct
     7441 ttatactccg ttcgtgatgt tctccaatct gtttcgcgcg aagcgcgagc tattcatgtc
     7501 gttagtcctc gtcgccggag tcggtgttct ggtgcctcgt ccgaccttcg ccgcacctcc
     7561 cccctccccc gccgtcatcg acgcccccag ccacatcacc atcgaagacc tggcccccgg
     7621 cgtcgtggcc gtcacgcgta atgaacccct gggcctcgcc aaccacgcca acagcatctt
     7681 catcatcggc gattcgagcg tcgtcgtcgt cgatgcccag ttcacctggg ccgccaccga
     7741 ggaagtcgtg gcggccctca aggcgcggac cgacaagccg gtgcgatatc tcgtcaacac
     7801 gcactggcac gacgaccacc tgttcgggaa cacggtctgg cgccggcact atccgggcgt
     7861 cgtgatcgtt tcgcacgaga aaacgcggga agacctgtcg tcgatcggcg cggagaaccg
     7921 caaggggcag ctcgagggtg ggcccgaagc ggtgacgatg ttcgaagacg ccatgaagaa
     7981 caaggtgacg atggaaggga cgccgatgag ccccgccgag ctcgacgcct accgcagcac
     8041 ggtggacatc gcgcggcagt acatcgccga ggcgccaggg aacgatccgc cattaccaga
     8101 tttggtattt cgcgaccgca tgacgatcta ccaggggaat cgcgcgatcg agcttcgcta
     8161 tttcggtgag gggatcacgc gcggcgacgc ggtcgtgtgg ctgccggccg agaagatcgc
     8221 ggtgacgggc gatctattca atgtgccgtt tcccttcgcg tacgggtgcc atcccacggc
     8281 gtggatcgcg gcgctgtcgg gaatccgcga gcttgggccg gccatcctcg tgccgggcca
     8341 cgggccggtg cagcgtgatc tcattgcgct caatcgccag gcggcggcgc tcgcggccat
     8401 tcgcgaggca gccgtgacgg ccgttgccaa cggcgtcacg gaagaggcgt tccgtcagtc
     8461 ggtgacgctg gaggactacc gccagccgat gacgggcgag aacaagatgc tcgggttcct
     8521 cttccggtcg tatttcgcgg gaccggcggc ggcggcagcg ttccgcgagg tcagcgcggc
     8581 gcgctgacgc ttcagatcgc ttaaactgcc ccgcgatgat ccccacactt ctgcgcgtcc
     8641 ggcgccagcg cctcgtcaac ctgatccgtt accacttcga ccggggcacc atcctctggg
     8701 tcggcctcac gatcgcgctg gtcgtcgtct ggcaatggcc gcatctccac ctccccacga
     8761 tctggcggga ccggacagcc cggggcgcgc ccgaccgctg gttcttcggt gcgctcggtt
     8821 ggttgccggt cctggccgtc acgttctacg cgctggcgcg cggcgtgaag gcgcgctcgg
     8881 cggagtactt ctggctgcac ctcgttgggg caccgaagtc gctcacacgg cgctggatga
     8941 tcctgcgtca gttgatgcgg accctcccga tcatcctcgc cgtggccatt cccacgatgc
     9001 tgccgggcgg cgcgccgggc ggctggtggc atgggccggt gctcatcgtg ggctacaccg
     9061 tcgtggccct gatcgcgctc atggccgtcc acctggggcc ggaagacgaa acgcggatgg
     9121 agcggaagga gcgcgagcgg aagacgcgtg accaggtaga gtcgttccgc atcgtgccgc
     9181 ggggccctct ctcggcgctt ctcgtacgcg acctggctta tctccagcgt ggcgcggccg
     9241 gctcgctcgg gatgtgcggc gccgctgcca tcggcgagtt cttcgttgcg caatcggcgc
     9301 tctccacacc gtcgggcacg ctggccatcc tgatcgtgca tgccatcgcg gcgctcctca
     9361 cggtgaacgg actcctggcg ctcttcgagc atgacgcggc gcatccgggg ctcctgcgca
     9421 cgctgccgat tccggggcgg cgcttctggg ccgcccgggt gctcatcgcc atgcttctct
     9481 gtgcggtgcc tatcgtgccg gcgttcgtcc tggcggtgct ggtgccgctc cccctggtcg
     9541 agctcgtggc gatcgcggga ctcggtttcg tcgtgatccc ggcggtggcc ggcatcctgt
     9601 acgccaacat cggcgtggcg ctcttc
//
LOCUS       DASVOD010000758         4174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_832929, whole genome shotgun sequence.
ACCESSION   DASVOD010000758 DASVOD010000000
VERSION     DASVOD010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4174
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832929"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF720_16245"
     CDS             complement(<1..643)
                     /locus_tag="VF720_16245"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7880963.1"
                     /translation="MAANDTRSPDPTLADTIAATASGPGTGTRVPSSEMPEQIGDYRI
                     LRILGAGGMGVVYLAEQQGSLRRQVALKVIRPDFATDDVLSRFENERRSLALMNHESI
                     ARVHDAGTTSAGQPYFVMEYVDGPSITRYCDERRLSVDERLELFIRVCHGVQHAHQKA
                     VIHRDLKPSNILVVEQDGRPVPKIIDFGVAKATAQRPSDATMVTRQGQWIGTLE"
     gene            913..1230
                     /gene="rplU"
                     /locus_tag="VF720_16250"
     CDS             913..1230
                     /gene="rplU"
                     /locus_tag="VF720_16250"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX7880964.1"
                     /translation="MYAIVGVAGFQYKVAKDQVVRVPLMSNLEAGQKITLDNVLLISD
                     GSSTEVGRPSVSGATVEAEVVKHGRTRKQLAAVYKKRKDYRRRWGFRAEFTMIKIDAI
                     KRG"
     gene            1254..1538
                     /gene="rpmA"
                     /locus_tag="VF720_16255"
     CDS             1254..1538
                     /gene="rpmA"
                     /locus_tag="VF720_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX7880965.1"
                     /translation="MAHKKGVGSSRNGRDSNAQRLGVKAYGGQKISAGTIIIRQRGTK
                     IHPGANVLKGKDDTLFATIDGYVVFGRKGRDRKMVSVLLERPVPAAAEAN"
     gene            1542..2462
                     /gene="mdh"
                     /locus_tag="VF720_16260"
     CDS             1542..2462
                     /gene="mdh"
                     /locus_tag="VF720_16260"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX7880966.1"
                     /translation="MRKVSVIGGGNVGASAALYIAERGIADVTLVDIVEGMPQGKALD
                     LRQSRSVRGFDPKITGTNNFADIAGSDVVLQTAGFPRKPGMSRTDLLKANAEIIENAA
                     SAIREHAPNAVVICVTNPLDAMTWLMYKRTGFPAERVVGMAGVLDSARFCCFIADELG
                     VSPNDVQAMVLGGHGDSMVPLPRYTTVSGISLPDLLPKETIDALARRTANGGAEIVGL
                     LKTGSAYYAPAASAAQMVEAVLLDRKRILPAAAFLDGVYGLKNVYCGVPIKLGAKGVE
                     QIIELKLAPEELAALQKSAADVSAMIAELG"
     gene            2572..3333
                     /locus_tag="VF720_16265"
     CDS             2572..3333
                     /locus_tag="VF720_16265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX7880967.1"
                     /translation="MTTVRSLVVWILCFVVLIFLGTPFLILSLFDNGGVRIYPISVLY
                     MRCVAFLAGLKVRALGKERVPFDATYVLMSNHRSWFDITAIMIATWPMQIRFVAKKEL
                     ADIPLFGACIKRGGHVMIDRKDRDSAIRTLRETARKFAGRFSIVVFPEGTRSPNHALL
                     PFKRGGFHLARELNIPVVPVSVVGGDQIMKRGTINLYPGTMRVMFHAPLLPSDYTSLD
                     ELQRAVRERIGAGLGEMGEFTADAPKVVAAAGVDG"
     gene            complement(3729..>4174)
                     /locus_tag="VF720_16270"
     CDS             complement(3729..>4174)
                     /locus_tag="VF720_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX7880968.1"
                     /translation="EFELAIHEQAEKGEPLTGENLADTYLKLVRKYYGHDKGVCKVDE
                     LYGMEWGFVNHFYYNFYVFQYATSMIASTSLAHGILEEKQLGEGRTTRRDAYLKMLSS
                     GSSEYPVDLLREAGVDMTTSAPFKAAMKEMNSVMDEMEKILAKKG"
BASE COUNT          707 a         1360 c         1369 g          738 t
ORIGIN      
        1 attcgagcgt gccgatccac tgtccctgcc gcgtcaccat cgtggcgtct gacggacgct
       61 gcgcggtggc cttggccacc ccgaagtcga tgatcttggg aaccggacgc ccgtcctgct
      121 cgaccaccag gatgttcgac ggcttgagat cccggtggat caccgccttc tggtgggcgt
      181 gctgcacgcc gtggcaaacg cggatgaaga gttcgagtcg ttcgtccacc gacaggcgac
      241 gctcgtcgca gtagcgcgta atcgacggcc cgtcgacata ctccatgaca aagtagggct
      301 ggcccgccga cgtggtcccc gcgtcgtgca cgcgggcgat cgactcgtgg ttcatcagcg
      361 ccagcgagcg acgctcgttc tcgaatcggc tcagcacatc gtcggtggcg aagtcggggc
      421 ggatgacctt gagggccacc tgccgccgga gcgatccctg ctgctcggcc aggtagacga
      481 cgcccatgcc cccggcgccg aggatgcgca ggatccggta gtcgccgatc tgttcgggca
      541 tctcggatga tggaacacga gtcccggtgc ccggaccgga tgcggtggcg gcgatggtgt
      601 cggcgagagt cgggtcgggc gagcgggtgt cgttggcggc catcgcggca gggtagcaga
      661 gtctaacggc cggagatatg gcataagtta ccgaaatgca acaggttgga gggcctgcga
      721 cagaaggagg gggtgtgtgc tgccgcgttg gcaggggcgc ctggcccacc gtcaccaccg
      781 gctatcggac aacccacgca aaaacgattg actcaggtcg tgttgcctac taaactccag
      841 cgcttgtttc ggttaagaca ttttcggggg taatgcgttc cgcggcctcc ggagttccag
      901 ggaggaccaa tcatgtacgc catcgtcggc gtggccgggt tccagtacaa ggtggccaag
      961 gaccaggtgg tccgtgttcc actcatgtcg aacctcgagg ccggccagaa gatcacgctc
     1021 gataacgtcc tgctcatttc cgacggctcc tcgaccgagg tcggccgccc gagcgtgtca
     1081 ggtgccaccg tcgaagccga agtcgtgaag catggccgca cgcggaagca gctcgcggcc
     1141 gtctacaaga agcgcaagga ttatcgccgt cggtggggat tccgcgccga gttcaccatg
     1201 atcaagatcg acgccatcaa gcgcggctga tcgactgaaa ggattcgtga tccatggctc
     1261 ataaaaaagg tgtcggctcg tcccgcaacg ggcgcgacag caatgcgcag cgcctcggcg
     1321 tgaaggctta cggcggccag aagatctccg ccggcacgat catcattcgc cagcgcggca
     1381 cgaagattca ccccggtgcc aacgtgctca aggggaagga cgatacgctc tttgccacga
     1441 tcgacggtta cgtggtgttc gggcgcaagg gccgcgatcg gaagatggtg tcggtgctcc
     1501 tcgagcgccc cgtgcctgcg gccgccgaag ccaactgatc gatgcgtaaa gtcagcgtca
     1561 tcggaggcgg caacgtcggc gcctccgccg cgctctatat tgccgagcgc ggaatcgccg
     1621 acgtcacgct ggtcgacatc gtcgagggaa tgccgcaggg gaaggccctc gacctccgcc
     1681 agagccgctc ggtccgcggc ttcgacccga aaatcaccgg cacgaacaat ttcgccgaca
     1741 tcgccggctc cgacgtcgtc ctccagacgg ccggcttccc ccgcaagccg gggatgagcc
     1801 gcaccgacct cctcaaggca aacgccgaga tcatcgagaa cgcggcctcg gccattcgcg
     1861 agcacgcccc gaacgcggtc gtcatctgcg tgacgaaccc gctcgatgcg atgacatggc
     1921 tgatgtacaa gcgaaccggc ttcccggccg agcgcgtggt gggcatggca ggtgtcctcg
     1981 attcggcccg cttctgctgc ttcatcgccg acgaactcgg cgtctcgccg aacgacgtcc
     2041 aggccatggt cctgggcggt cacggcgact cgatggtgcc gctgccgcgc tacaccacgg
     2101 tgtcgggcat ctcgcttccc gatctcctcc cgaaggagac gatcgacgcg ctcgccaggc
     2161 ggaccgccaa cggcggcgcc gagatcgtgg gcctcctgaa gaccggcagc gcctactacg
     2221 cgccggccgc cagcgccgcc cagatggtcg aggccgtcct gctcgaccgg aagcgcatcc
     2281 tgcccgccgc ggcgttcctc gatggcgtgt acgggctcaa gaacgtctac tgcggcgtgc
     2341 cgatcaagct gggcgcgaag ggcgtcgagc agatcatcga attgaagctc gcgccggagg
     2401 agctggcggc gctccagaag tcggcggccg acgtgtcggc catgatcgcc gagctgggtt
     2461 agcgctttcc gcggcggctt cggccggcgc gtcacgacga aaacttcatg gaagggcgcc
     2521 ggccaccgcc ggcgccctga ctttttgggg tcgtccccgt acaatgtggg catgacgaca
     2581 gtccgctcgc tggttgtctg gatcctctgc ttcgtcgtcc tcatctttct cggtacgccg
     2641 ttcctcatcc tctcgctctt cgacaacggc ggcgtccgca tctaccccat cagcgtcctc
     2701 tacatgcgat gcgtggcgtt cctggcgggg ctcaaagtcc gtgcgctcgg caaggagcgc
     2761 gttcccttcg atgccaccta cgtcctcatg tcgaaccacc ggtcctggtt cgacatcacc
     2821 gccatcatga tcgccacctg gccgatgcag atccgcttcg tggccaagaa ggagcttgcc
     2881 gacatcccgc tgttcggcgc ctgcatcaag cgcggcggcc acgtgatgat cgaccgcaag
     2941 gaccgcgact cggccatccg cacgctgcgg gaaacggccc ggaaattcgc cgggagattc
     3001 tcgatcgtgg tcttccccga aggcacccgc agccccaacc acgcgctcct gccgttcaag
     3061 cgcggcggct tccacctggc tcgtgagctg aacatcccgg ttgtgcccgt gtcggttgtc
     3121 ggcggcgatc aaatcatgaa gcgcggcacc atcaacctct acccgggcac gatgcgggtg
     3181 atgttccacg ctccgctcct gccgtcggat tacacgagcc tcgacgaact gcaaagggcc
     3241 gtgcgggagc ggatcggggc gggtttgggg gagatggggg agttcacggc ggatgcgccg
     3301 aaggtggtgg cggctgcggg agtcgacggt tgagtcgctg ccggggctga cggtggggtg
     3361 gctgccgggg tcgacgcttg agttgctgac gggcggacgg tgtcggcgct gccggtgctc
     3421 ctggtggtgc cgctggtgag cgtgacgcct gagtgagggc ccgactggag aaaccgcatc
     3481 cggcccgccg cgtttgtgaa aaaaggtgca actcgcaccg gtgcgagttg tcccggcggc
     3541 caggatgcgc tcaagcccgg cagattcagg cggaagcacc tcgatcgata acagtgtcgg
     3601 taatgaaaac gcccggcccg gcaaagccgg accgggcgta cctcatgtcg ttctgacgac
     3661 tcgttcggac ggaacgctcg ccccgagcca tcgtcgggcg tgcgccgggc gcaccaggtc
     3721 gccgccgctc accccttctt cgccaggatc ttctccatct cgtccatgac ggaattcatc
     3781 tccttcatgg ccgccttgaa gggggcggac gtcgtcatgt ccacgcccgc ctcccgcagc
     3841 aggtccaccg ggtactccga cgagcccgac gacagcatct tgaggtaggc gtcgcgcctg
     3901 gtcgtcctcc cctcaccgag ctgcttctcc tcgaggatgc cgtgggcgag cgaggtcgag
     3961 gcgatcatgc tggtggcgta ctggaagacg tagaagttgt agtagaagtg gttcacgaag
     4021 ccccactcca ttccgtacag ctcgtcgacc ttgcacacgc ccttgtcgtg cccgtagtac
     4081 ttgcgcacga gcttgaggta ggtgtcggcc aggttctcgc cggtcagggg ctcgcccttc
     4141 tcggcctgct cgtggatggc cagctcgaac tcgg
//
LOCUS       DASVOD010000759         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_727106, whole genome shotgun sequence.
ACCESSION   DASVOD010000759 DASVOD010000000
VERSION     DASVOD010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727106"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1950)
                     /locus_tag="VF720_16275"
     CDS             complement(<1..1950)
                     /locus_tag="VF720_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020166153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX7880969.1"
                     /translation="MSTSLRIALGLVLIGAGSVAANTTPQVSLSQNWSNASLITINDD
                     WSQVPGIMGRLGDGLTNSGGIDPQTVLADQPTVDVIANQSVPGTLMAGGVAEFDGIAD
                     RVVALQGSGTADAPFLLLTLDTSGQSSVTVSYKLRDIDDSSDNALTPVALQYRIGTTG
                     DFTNIPAGYVADATTGPNLATLVTPVSVVLPAAVSNRPVVQLRIITTNAVSTDEWVGI
                     DDIQVTAGPSCFSDITSGLPPLRASRVAWGDYDNDTDMDLLVTGDIGGSVVTRVYRSS
                     GGLNPVFHDITAPLPGLIGPEVAWVDYDVDGDLDIFVSGATADSSLTRIYRNNGIPNP
                     TFTDAQAGLPGMPAGAAAWGDYDNDGDPDLLQTGQYGRGQPYATRLYRNDRGAVPVFT
                     LVPTNLPGAREGDVAWGDYDNDGDLDILMAGITEPGTLPLVFRNDGDEVFTSIGAGDS
                     DNSGVAVDWGDYDADGDLDALVARQNYIDIRTNQVAGMVHSFAAVLPPYTPGVMQGTT
                     TWGDYDNDGRLDFLSTGYDFWAFRPATNLFRQVPASPYFEYVGTGMPGIGDDPTPDGL
                     AGGAWGDADGDLDLDVVLAGGAFTGVPTTRVYRNVCGANTAPTAPTGLSVTVVGLRAT
                     FSWSAATDAETPAAGLSYNLRVGTAP"
BASE COUNT          303 a          694 c          707 g          309 t
ORIGIN      
        1 gggggccgtg ccaacgcgca ggttgtacga gagcccggcc gcgggcgtct cggcgtcggt
       61 ggcggcgctc cacgaaaacg tggcccggag gccgacgacg gtcacggaga gcccggtcgg
      121 cgctgtcggc gcggtgttgg cgccgcagac gttgcggtac acccgcgtcg tcggcacgcc
      181 ggtgaaggcg ccgccggcga ggaccacgtc caggtccagg tcaccgtcgg cgtcgcccca
      241 ggcgccaccg gcaagcccgt cgggcgtcgg gtcgtcgccg atccccggca tgccggtgcc
      301 cacgtattcg aagtacggcg aggccggcac ctggcggaag aggttcgtgg cgggacggaa
      361 cgcccagaag tcgtagccgg tggataggaa gtcgagccgg ccgtcattgt cgtagtcgcc
      421 ccaggtggtc gtcccctgca tcacgcccgg cgtgtacggc ggcaggaccg cggcgaacga
      481 gtgaaccatg cccgccacct ggttcgtccg gatgtcgatg tagttctgcc gcgccacgag
      541 cgcgtcgagg tcgccgtcgg cgtcgtaatc gccccagtcc accgccacgc cggagttgtc
      601 gctgtcgccg gcgccgatcg aggtgaagac ctcgtccccg tcgttccgga aaaccagcgg
      661 cagcgtgccc ggctcggtga tcccggccat caggatgtcg aggtcgccgt cgttgtcgta
      721 gtcgccccag gccacgtccc cttcgcgcgc gcccggcagg ttggtcggca ccagcgtgaa
      781 caccggcacc gcgccccgat cgttccggta gagccgcgtc gcgtacggct gtccccggcc
      841 gtattgcccc gtctgcagca ggtccggatc gccgtcgttg tcgtaatcgc cccacgccgc
      901 ggccccggcc ggcatgcccg gcagtcccgc ctgcgcgtcg gtgaacgtcg ggttgggaat
      961 gccgttgttc cggtagatgc gggtaaggct cgagtcggcc gtcgcgcccg acacgaagat
     1021 gtcgagatcg ccgtccacgt cgtaatccac ccaggccacc tcggggccga tgagccccgg
     1081 caggggggcc gtgatgtcgt ggaataccgg gttcaggccg ccgctgctgc ggtacacgcg
     1141 cgtcaccacc gacccgccga tgtcccctgt cacgaggagg tccatgtcgg tgtcgttgtc
     1201 gtagtcgccc caggccaccc ggctcgcgcg cagcggcggc aggccggagg tgatgtcgga
     1261 aaagcacgaa ggaccggccg tcacctgaat gtcgtcgatc ccgacccact cgtccgtgct
     1321 gaccgcgttg gtggtgatga tccggagctg gacgaccggc cggttgctca cggcggccgg
     1381 caacacgacc gacaccggcg tcaccagcgt ggccaggttc ggccccgtcg tggcatcggc
     1441 cacgtagccg gcggggatgt tggtgaagtc tccggtcgta ccgatccggt actgcagcgc
     1501 caccggcgtc agcgcgttgt cggaagagtc atcgatgtcg cgcagcttgt acgaaacggt
     1561 gaccgacgat tgccccgacg tgtcgagcgt caacagcagg aaaggggcat cggcagtgcc
     1621 gctcccctgc agcgccacga cgcggtcggc aatgccgtcg aactcggcca cgcccccggc
     1681 catcagcgtg ccgggaaccg actggttggc gatgacgtcg acggtcggct ggtcggcgag
     1741 cacggtctgc gggtcgatcc cgcccgagtt ggtcaggcca tcgcccagac gccccatgat
     1801 ccccggcacc tgggaccagt cgtcgttgat tgtgatcagg ctcgcgttcg accagttctg
     1861 cgaaagcgac acctgggggg tggtgttggc cgccacgctc ccagcgccga tcagcaccag
     1921 ccccagggcg attcgaaggg aagtcgacac ggtaacctcc tgctgcagcg caagttcgga
     1981 tgcctgccgg aagggccggc agaaccgccg caa
//
LOCUS       DASVOD010000760         8407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_311872, whole genome shotgun sequence.
ACCESSION   DASVOD010000760 DASVOD010000000
VERSION     DASVOD010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8407
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311872"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VF720_16280"
     CDS             <1..601
                     /locus_tag="VF720_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880970.1"
                     /translation="PGQVRYRGSAHTIHRVVRLLGFRGLRIPHGMPIDSSVISPKDVF
                     AGYLMLDAWIGNQDRHHENWGVIITPTGEVFLAPTFDHASSLGRNETDANRAKRLATR
                     DVGFGLERYVERAQTPLYESETSPTPLTTLGAFLGAARFSPKASKVWLDRMMAIPDTE
                     IVSIFERIPRTLISDVAIEFAIKMLQLNRARLAKAGVSL"
     gene            complement(1440..2333)
                     /locus_tag="VF720_16285"
     CDS             complement(1440..2333)
                     /locus_tag="VF720_16285"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX7880971.1"
                     /translation="MTVDFRAARLEGLLACPDCRTDLPGQPTACPTCGRRFTVEAGIP
                     VMHPKGSASADSGRQFFVDDPGRMDQLRRKAPWLARMLELPDFTSPSGALPKCREWHR
                     AHVIDGMPGQRVLNLGSGVHKQYLNPGLINFDIAAHDNVDVVGDGEHLPFRDGVFDGV
                     VLDAVIEHLARPARVVAEVKRVLKPGGTVLAQVPFLYPFHAAPHDYQRYTPAGLGMLF
                     EEFDVLETGTDRRPGRAVLEVVSAWAAGFSDNRTVSYALRWLTAWVWLPIKWLDPWLG
                     KKSRGEYVAASGSVLARKRAG"
     gene            2702..4033
                     /gene="lat"
                     /locus_tag="VF720_16290"
     CDS             2702..4033
                     /gene="lat"
                     /locus_tag="VF720_16290"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017259182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HEX7880972.1"
                     /translation="MTVRVPEVHETLKKTMLVDGYPLVVDLEKSHSSYLVDAATGDEY
                     LDFFSYFATNPIGHNHPRINNSEFKRHMGEVAIHNPSNADFYTTVMADFVDTFRRLAM
                     PREIKHVFWISGGSLGVENALKAAFDWKVRKNAAKGYEEELGSRIIHFRHAFHGRSGY
                     TLSLTNTDPNKTAFFPKFEWPRISHPKIRFPMDGANQRLVMQAEQQSLAEIEAAIERH
                     GDDIAALIIEPIQAEGGDNHVRGEFLRELRRLADQHEFLLIFDEVQTGVGMTGKMWAF
                     EHFDVVPDLIAFGKKMQICGFMSTGRIDDVDSVFKVPSRINSTWGGNLVDMARSQRYL
                     EIIAEENLVENSRLRGHYLVENLEELQRDFPKLVSQPRGRGLMCAFDLPSADLRAKFL
                     DDCFDKKLIILPCGTSSVRFRPSLTVTDAEIEAGIGIIRKVLQDLSRQERV"
     gene            4036..5046
                     /gene="fbp"
                     /locus_tag="VF720_16295"
     CDS             4036..5046
                     /gene="fbp"
                     /locus_tag="VF720_16295"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011357224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEX7880973.1"
                     /translation="MVNKVVTISRHISEMERNFPSATGDFSGLMHDITLAAKMIQREV
                     TKAGLVNILGKAGDTNVQGEEQQKLDVFADLTMFRALDHGGHLCVMASEENEETLAIP
                     DSYPCGKYVLNYDPLDGSSNIDANVSIGTIFSVLRRCTPGDSHGNATDCMQPGFKQVA
                     AGYVMYGSSTMFVYTTGQGVHGFTLDPTFGEFLLSHENIRIPKRGKIYSVNEGNYHLW
                     DEGTRRYVDHVKAEQVDDGRPYSGRYIGSMVCDIHRTLLYGGVYLYPADRKNPQGKLR
                     LLYEANPMAFIVEQAGGAATDGRQRILEKTPDSLHQRTPLIVGSAEDVALYEKFAKNG
                     KG"
     gene            complement(5089..5328)
                     /locus_tag="VF720_16300"
     CDS             complement(5089..5328)
                     /locus_tag="VF720_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX7880974.1"
                     /translation="MASTKITIQSNGSMRIEGDFEIVDPAGTPYGLAGRTTVSLCRCG
                     QSANKPFCDGSHKRCEFQHQPVAFELPPPARPPGG"
     gene            complement(5349..5756)
                     /locus_tag="VF720_16305"
     CDS             complement(5349..5756)
                     /locus_tag="VF720_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEX7880975.1"
                     /translation="MTGDEEASRELVSTWMKATKDGDMKTVLDLMTDDVVFMTPGNPP
                     MVGRIAFETAARGGIRAGTIDGQSEVDEVCVVGDFAYLRSTLTVTMRPHSGGTPVRRV
                     GNTLSVLRREGGKWRIARDANMLAVVSTPSPAQ"
     gene            complement(5877..6338)
                     /locus_tag="VF720_16310"
     CDS             complement(5877..6338)
                     /locus_tag="VF720_16310"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX7880976.1"
                     /translation="MTDQLQLLIDRANIVDTINRLFVSTDRRDWPAVLGCLAERVQVD
                     MSSVGAGPAREMSGEELAGMWEAGLAPITAVHHQAGNHLVEVRGTTARAYCYAIATHW
                     RAVASGNNMRTFTGSYDIALTCEEMVWRISSFAFHLKYMEGNAQLETEPPA"
     gene            6437..>8407
                     /locus_tag="VF720_16315"
     CDS             6437..>8407
                     /locus_tag="VF720_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7880977.1"
                     /translation="MIAEQVSHYKILGRLGAGAMGEVFRAEDTRLGRQVALKFLRPDL
                     LGDERARQRMEFEARAAAILNHPHITTLYEFDAGEGFLSFEFVEGQTLEDRLEKPMDP
                     LLAVQTCLAICRGLAHAHGRNVVHRDLKPANVLIGNDGSVKLSDFGLAKVRDALAQAT
                     QEIAQGTPAYMAPERISGQGSDHRGDLFALGVLVYRALAGKLPWPGESLMEVLFAVLQ
                     HEPEPLETAVPELPPSLSELVARLMDKEPETRLGSANLAETYLLEAEAWLLLPPEKRV
                     KAPPPAPAAPAVPTKGAPASSAGKGTLTPKAPTRDGERAELPMIGRDDELATMERLIG
                     EMQGGTGRPVLVRGTAGAGKTRFVDEVMKRHRKDRVLFLRGRCHQHGNRNFGALVDAL
                     EGFARGVVDGGDRYLADLTGDLEAIGGLLPTLRMLLDDAPAGGQPKTREELWFLLESL
                     LKRIADVVPLVLVVDDLHWADEGTVALTWHLGRNIAESRLMLLGMFRPEELVDPAGRT
                     RESGEMVRGLAETQGLEVIELAPLDENGTTALVRAAFPDSPLATSLAPLIHRRAQGNP
                     MFTVEIVRLISTPDPTAMSTDQSSDETSKALLPRNIADVVARRLARLSNEERELLDVA
                     AVEGAIFHADVVAEATGMPRLKLLSRLRDLART"
BASE COUNT         1520 a         2818 c         2667 g         1402 t
ORIGIN      
        1 ccccgggcaa gttcgatatc gcggcagcgc gcatactatc catcgagttg tgcggctact
       61 gggctttcga ggcctgagga ttccgcatgg aatgccgata gattcaagtg tgatctctcc
      121 gaaggatgtg tttgcgggct atctgatgct ggacgcctgg atcggaaatc aagatagaca
      181 tcacgagaac tggggcgtga taatcacgcc gacgggggag gtattccttg cgcctacgtt
      241 tgatcacgct tcgagccttg gccgaaatga aacggatgcg aatcgagcta agcggctggc
      301 tactcgtgac gtcggatttg gcctcgaacg gtatgtcgag cgagctcaga ccccactcta
      361 tgaatccgag acgagtccaa ccccgctgac taccctcggg gcgttcttgg gcgccgccag
      421 attttcgccg aaggcctcga aggtgtggct cgaccgcatg atggcaatcc ctgacacgga
      481 gatcgtttcg atcttcgaga gaattccacg taccttgatc tccgacgtgg cgattgagtt
      541 tgcaataaag atgctgcagc tgaatcgtgc gcgacttgcg aaggcgggag tgagcttgtg
      601 agacgagaat tgcttgtagc gtggcagcag ccagactctc gtcggtggat ccccgttggt
      661 cgactgcgtg ccacgccggc ctactacgag ttcgtgttta cagccggtgc gcagcaggct
      721 gaggcatttc gaccgttcgg taggaaccga gacttgacga gggtttaccg ttcaacggaa
      781 ctgttcccaa ttttcgcaaa ccgagttctc tccaagggac gtcccgaata tcaggacttc
      841 ttggggtggc tagatcttga ggcctctgac ccgatcgcgt tgctgggtct gactggtgga
      901 gagcgcgaaa ctgactcatt gatcgtattc ccgatcccat tccccgatcc ggccggcaat
      961 taccaagttc aattctttag tcatggaatg cgctacttgt ctgagattga aagggaacaa
     1021 cagaagcttc tcacccccgg acaacgactc gaacttttgc cagagcctga aaacgcggaa
     1081 gatcgatacg ctattcgact tgtggtcggg gaaacaagca acaaagttgg ctattgccca
     1141 cggtatctct gcccagacgt gcacagactg ctaagtctcg tcgggaatga tcaggtcctg
     1201 acggaggtgg ttcgtgtaaa cgttttcgcg ccaattcaat tccggcttct ttgtaaattg
     1261 actgcgccgt ggccggccga gttcatccca tgtcaggacc cgctggcaga accgctcgta
     1321 gaattcggaa attcggacac tggagatgcg agtcgagccg tatcctaaga acgtctttgg
     1381 ccggtgaatg cttgtaccaa aaacctgaaa aacctcactt ggggctgtac cttaatggcc
     1441 tagcccgccc gcttccgtgc cagcaccgac ccgctcgccg ccacgtactc cccgcgcgac
     1501 ttcttcccca gccacgggtc gagccacttg atcgggagcc acacccaggc ggtgagccag
     1561 cgcagggcgt acgacacggt gcggttgtcg ctgaagccgg cggcccaggc ggacacgact
     1621 tcgaggacgg cgcggccagg gcggcggtcg gtgccggttt ccagcacgtc gaactcctcg
     1681 aacagcattc ccagcccagc cggcgtgtag cgctggtagt cgtgcggcgc ggcgtgaaac
     1741 ggatagagga acggcacctg ggcaagcacg gtgccgccgg gcttgaggac gcgcttcact
     1801 tcggccacca cgcgggccgg acgggccagg tgctcgatga cggcatcgag cacgacgccg
     1861 tcgaagacgc cgtcgcggaa cggaaggtgc tcgccgtcgc cgacgacgtc cacgttgtcg
     1921 tgcgcggcga tgtcgaagtt gatcaatccg ggattcaggt actgcttgtg cacgcccgat
     1981 ccgaggttga gcacccgttg accgggcatg ccgtcgatca cgtgcgcgcg gtgccactcg
     2041 cggcactttg gcagggcccc gctcggcgac gtgaagtccg gcagctccag catccgcgcc
     2101 agccagggtg ccttccggcg gagctggtcc atccggccgg ggtcgtccac gaagaactgc
     2161 cggccggagt cggcgctggc gctccccttc gggtgcatca ccgggatgcc cgcctccacc
     2221 gtgaaacggc gaccgcaggt ggggcacgcg gtgggttggc ccggcaggtc ggtgcggcag
     2281 tcgggacagg ccagcagtcc ctccagccgg gcggcccgga aatcgacggt catcttggcg
     2341 gcaaccccat tcccatcaat gagttaaggc aacagatcgc tttgacctga ccggcggcgg
     2401 gatgctacca tagcagccgc caagcgccgg tgcgggtgac gcgagcaccc cggccggcat
     2461 ctcgtgatcg acccatccgg gggcctccat gaccagccag gtgagttcct tccatctcct
     2521 gcgcccccac ctgtcgttcc ggctctgcgc gttccggggt tggggctaag cagcaatcag
     2581 tcccggtcga taccggactc gaactgtttc gcaccctgtt ttcacccgac agacgcccga
     2641 cgcccgacaa gcccggtgcc ccggcccatc ggccgcaccg tccggaacga aaggaaccgc
     2701 catgaccgtc cgcgtacccg aagtccacga gaccctgaag aaaacgatgc tcgtcgacgg
     2761 ctatccgctc gtcgtcgacc tcgagaaatc acactcctcg tacctggtgg atgccgccac
     2821 cggcgacgag tacctcgact tcttcagcta ctttgccacc aacccgatcg gccacaacca
     2881 cccccgcatc aacaattccg aattcaagcg gcacatgggg gaggtggcca ttcacaaccc
     2941 ctcgaacgcc gatttctaca cgacggtgat ggccgacttc gtcgacacct tccggcggct
     3001 ggccatgccc cgggaaatca agcacgtctt ctggatctcg ggcggatcgc tgggggtcga
     3061 gaacgcgctc aaggccgcct tcgactggaa agtgcggaag aacgccgcca aggggtacga
     3121 ggaggagctc ggcagccgca tcatccactt ccggcacgcc ttccacggcc gctcgggcta
     3181 cacgctgagc ctcaccaaca ccgacccgaa caagacggcc ttcttcccca agttcgagtg
     3241 gccgcgcatc agccatccca agatccgctt tccgatggat ggcgccaacc agcggctcgt
     3301 catgcaggcc gagcagcagt cgctggccga gatcgaggcc gccatcgagc gccacggcga
     3361 cgacatcgcc gccctcatca tcgagccgat ccaggccgag gggggcgaca accatgtccg
     3421 cggcgaattc ctgcgcgagc tgcgccggct ggccgaccag cacgaattcc tcctcatctt
     3481 cgacgaggtg cagaccgggg tcggcatgac cgggaagatg tgggcgttcg agcatttcga
     3541 cgtcgtgccc gacctcatcg ctttcggcaa gaagatgcag atctgcggtt tcatgtcgac
     3601 cggccgcatc gacgacgtgg actcggtctt caaggtgccg agccgcatca actccacgtg
     3661 gggcggcaac ctggtggaca tggcgcgcag ccagcgctac ctcgagatca tcgcggaaga
     3721 aaacctggtg gagaacagcc gccttcgcgg ccactacctc gtggaaaacc tcgaggagtt
     3781 gcagcgcgac ttcccgaaac tggtcagcca gccacgcggg cgcggcctca tgtgcgcctt
     3841 cgacctgccg tccgccgacc tccgcgcgaa gtttctcgac gactgcttcg acaagaagct
     3901 catcatcctg ccttgcggca cgagctcggt gcgcttccgg cccagcctca ccgtcaccga
     3961 cgcggagatc gaggccggca tcggcatcat ccgcaaggtg ctccaggacc tctcacgcca
     4021 ggaaagggtg tgaccatggt caacaaggtc gtcacgatct cgcgccacat cagcgagatg
     4081 gagcgcaact ttcccagcgc caccggcgat ttctccggcc tcatgcacga catcacgctg
     4141 gccgccaaga tgatccagcg cgaagtgacc aaggcgggcc tcgtgaacat cctcggcaag
     4201 gcgggcgaca ccaacgtgca gggggaggag cagcagaagc tcgacgtctt cgccgacctc
     4261 accatgttcc gggcgttgga ccatggcggg cacctctgcg tcatggccag cgaggaaaac
     4321 gaggagacgc tggccattcc cgattcttac ccgtgcggca agtacgtgct gaattacgac
     4381 ccgctcgacg gctcctccaa catcgatgcc aacgtgtcga tcggcaccat cttctcggtg
     4441 ctccgccgat gcacgccggg cgacagccac ggaaatgcca ccgactgcat gcaacccgga
     4501 ttcaagcagg tggcggccgg ctacgtgatg tacggctcga gcaccatgtt cgtgtacacc
     4561 accggccagg gggtgcacgg cttcacgctc gaccccacct tcggcgaatt tcttctgtcg
     4621 cacgagaaca tccgcatccc gaagcgcggg aagatctact ccgtgaatga ggggaactat
     4681 cacctgtggg acgagggaac gcggcgttac gtggaccacg tgaaggccga gcaggtggac
     4741 gacggccgcc cctactcggg ccgctacatc ggttcgatgg tgtgcgacat ccaccgcacg
     4801 ctcctgtacg gcggcgtgta cctctacccg gccgaccgca agaacccgca gggaaagctc
     4861 cgccttctgt acgaagccaa cccgatggcc ttcatcgtgg agcaggccgg cggcgccgcc
     4921 accgacggcc gccagcgcat tctggaaaag acccccgaca gcctgcacca gcgcaccccc
     4981 ctcatcgtcg gcagtgcgga agatgtggcg ctgtacgaga agttcgcgaa gaacgggaaa
     5041 gggtgatcac gcggcgggag tgaaccgccc ggcggcgtca gcccccggtt acccgcccgg
     5101 cggacgcgcc ggcggcggca gctcgaacgc caccggctgg tgctggaact cgcagcgctt
     5161 gtgcgagccg tcgcagaacg gcttgttggc cgactgcccg cagcggcaga gcgagacggt
     5221 cgttcgcccc gccagcccgt aaggcgtgcc ggcaggatcg acgatctcga aatccccctc
     5281 gatccgcatc gaaccgttgg actggatggt gatcttcgtc gacgccattc gagagcctcc
     5341 tccagggatt attgcgccgg cgacggcgtg ctcaccaccg ccagcatgtt cgcgtcgcgc
     5401 gcgatgcgcc acttgccgcc ttcgcggcgc agtaccgaca gcgtgttgcc gacccgacgg
     5461 accggcgtgc ccccggagtg cggcctcatc gtgacagtca gcgtgctgcg caggtacgcg
     5521 aagtcgccca ccacacagac ctcgtcgacc tcgctctgcc cgtcaatggt accggcccgg
     5581 atgccgccgc gcgccgccgt ctcgaacgcg atccggccca ccatgggtgg attgcccggc
     5641 gtcatgaaga ccacgtcgtc cgtcatcagg tcgagcaccg tcttcatgtc accgtccttc
     5701 gttgccttca tccaggtgct caccagctcc cggctggctt cctcatcgcc cgtcatcgtt
     5761 gtgcactccc gttcagaatc aacgttgcga gcggccatga atcccggaga atgtcaaaac
     5821 cggcgtgacg agggaagtgg gcgatgggga gatgccccag ccggacgggc gatgcgtcag
     5881 gccggcggct ccgtctcgag ctgcgcattc ccctccatgt acttgaggtg gaacgcgaac
     5941 gacgaaatcc gccagaccat ctcctcgcag gtcagcgcaa tgtcgtagct ccccgtgaac
     6001 gtccgcatgt tgttccccga ggccacggcc cgccagtggg tggcgatggc gtagcagtag
     6061 gccctggcgg tcgtcccgcg cacttccacc aggtgattgc cggcctggtg atgcacggcg
     6121 gtgatggggg caagccccgc ctcccacatt cctgccagct cctcgcccga catttcccgg
     6181 gccgggccgg cgccgaccga cgacatgtcg acctggactc gctccgcgag acaccccaac
     6241 acggcgggcc agtcccgtcg gtcggtggaa acgaagaggc gattgatcgt gtcgacgatg
     6301 ttggcgcggt cgatcaggag ctggagctgg tcggtcatgg ggtccttccg gggcggccct
     6361 tccggcgcgg gccgcgaaat cgcgtgtaac ggcgcccccg tggcgtgccg ggccacctgt
     6421 tattctctca cccgccatga tcgccgagca ggtatcgcac tacaagattc tgggacgcct
     6481 cggggccggg gccatgggtg aggtgttccg ggccgaggat acccgcctgg ggcgccaggt
     6541 ggccctcaag tttctccgcc ccgatctcct cggggacgag cgggcccgcc agcgcatgga
     6601 gttcgaggcg cgcgcggcgg ccatcctcaa ccatccccac atcaccaccc tgtacgagtt
     6661 cgacgcgggt gaggggttcc tctccttcga attcgtggag gggcagaccc tcgaggatcg
     6721 gctcgagaag ccgatggatc cgctcctggc ggtgcagacc tgcctggcca tctgccgggg
     6781 gctggcacac gcgcacgggc gcaacgtcgt gcatcgcgac ctgaagccgg ccaacgtcct
     6841 catcggcaac gacggcagcg tcaagctcag cgacttcggc ctggccaagg tgcgcgacgc
     6901 gctggcgcag gccacccagg aaatcgccca gggaacgccc gcctacatgg cgccggagcg
     6961 gatctcgggg cagggctccg atcatcgcgg cgatcttttc gcgctcggcg tgctggtgta
     7021 ccgggcgctg gcgggaaagc tgccgtggcc gggcgagagc ctgatggaag tgctcttcgc
     7081 ggtgctccag cacgagccgg agccgctcga gacggccgtg cccgagctgc cgccgtcact
     7141 ctccgagctg gtcgcgcgcc tcatggacaa ggagcccgag acgcgcctcg gctcggccaa
     7201 cctggcggag acctacctgc tcgaggccga ggcgtggctc ctcctgccgc cggaaaagcg
     7261 ggtaaaggcg ccgccgccgg cgccggccgc gccagccgtt ccgacgaagg gcgccccggc
     7321 ctcgtcggcc ggcaagggga cactcacacc gaaagcgccc acgcgcgacg gcgagcgggc
     7381 cgagctcccg atgatcggtc gcgacgacga actggccacc atggaacggc tcatcggcga
     7441 gatgcagggg ggcaccgggc ggccggtgct ggtgcgcggc acggccggcg ccggcaagac
     7501 gcgcttcgtg gacgaggtga tgaagcgcca ccggaaggac cgcgtcctct tcctgcgcgg
     7561 gcgctgccac cagcatggca atcgaaactt cggagcgctc gtcgacgcgc tggaaggatt
     7621 cgcgcgcggc gtggtggacg gcggcgatcg ctacctggcc gacctgacgg gcgacctcga
     7681 ggcgatcggc ggccttctcc ccacgctccg gatgctgctc gacgatgcgc cggccggcgg
     7741 ccagccgaag acgcgcgagg aactctggtt cctgctcgaa tcgcttctga agcggatcgc
     7801 cgacgtcgtg cccctcgtcc tggtggtgga cgaccttcac tgggccgacg aaggaaccgt
     7861 ggcgctcacc tggcacctcg gccgcaacat cgccgaatcg cggctgatgc tgctcgggat
     7921 gttccggccg gaggagctgg tcgacccggc cgggcgcacg cgcgaatcgg gggagatggt
     7981 gcggggcctg gccgaaacgc aggggctcga ggtcatcgaa ctggcgccgc tcgacgagaa
     8041 cggcaccacg gccctcgtgc gcgccgcctt cccggacagt ccgctggcca cctcgctggc
     8101 gccgctcatc catcggcgtg cccagggaaa ccccatgttc accgtcgaga tcgtgcgcct
     8161 catctcgacg cccgatccca cggccatgag caccgaccag tcgagcgacg agacgagcaa
     8221 ggcgctcctt ccccgcaaca tcgccgacgt ggtggcgcgc cgcctcgcac gtctgtccaa
     8281 cgaagagcgc gaactcctcg acgtggcggc cgtggaggga gccatcttcc acgcggacgt
     8341 cgtggccgaa gccaccggca tgccgcgtct caagctcctc tcgcggttgc gcgacctggc
     8401 caggacg
//
LOCUS       DASVOD010000761         6567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_362413, whole genome shotgun sequence.
ACCESSION   DASVOD010000761 DASVOD010000000
VERSION     DASVOD010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6567
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362413"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="VF720_16320"
     CDS             <1..955
                     /locus_tag="VF720_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEX7880978.1"
                     /translation="ELPPFKAAIDGGVQVVMTGHLACPALDPSGAPATLSPRILSGLL
                     RDELGFEGIIVSDDMEMSGVAALGDAGAVARNAIEAGVDMLLYARSRDMAEEVRESLA
                     DAIESGEIDEHRVLQSLARLGRLREAIRATRTEIDDTAREEVVDHEHEELLRAVAIQG
                     VTSLGPVGPELPVPWPDRYGLIIIPTADAPRLDVEVEYLRNLIEPLRIGLEVVSLRPT
                     TEQREAARYRASRADFLVACTLTRGAIVPEQRQLIDDLLILKKQLTVVSLLDPHDLSE
                     YPTVRTRLATRGFHRHALDALVHILTGLSEPRLDPASHQIP"
     gene            1009..1085
                     /locus_tag="VF720_16325"
     tRNA            1009..1085
                     /locus_tag="VF720_16325"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1043..1045,aa:Asp,seq:gtc)
     gene            1317..1559
                     /locus_tag="VF720_16330"
     CDS             1317..1559
                     /locus_tag="VF720_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HEX7880979.1"
                     /translation="MTQIPIVVRAEYRSAFRIHLTFNDGTAKTVDFNDWLEGPTFEAL
                     KSEDYFKEFFLDGGTVAWSNGADIAPETLYEARSAD"
     gene            1690..4368
                     /locus_tag="VF720_16335"
     CDS             1690..4368
                     /locus_tag="VF720_16335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX7880980.1"
                     /translation="MPLTAGTRLGPYEVVSPLGSGGMGEVYRARDTRLLRDVAVKVLP
                     EQLSANPDLRARFEREARAISSLNHPHICTLHDVGREGGIDYLVMEMVEGETLAARLE
                     RGPLPLPEVLRTGAQIADALDRAHRAGVVHRDLKPGNVMLTKSGAKLMDFGLARATGL
                     AGPMGEGGRNTALTQSPTLVQPLTAEGSIIGTFQYMSPEQLEGKEADARSDLWALGCV
                     LYEMATGRRAFDGRSQASLISSIMKEEPAPIGTTNPVHTGTAGFGLERLVSRCLAKSQ
                     DDRWQNAGDLKHELEWLASGQSSLGASVVAEAARAPATRRRRSETLAWVLAAAGIGTA
                     LVLYTRMERSSPGAGVMARVSLPLTQHLQLSSESCDIAISPDGRTVAFVAADSTDVDR
                     LWLRSLDSPTARMIPESESATSPFWSPDSRQVAFFGEGSRKLKKVSAAGGSPVVLADA
                     STPRGGSWSSKGVIVFAPAPEGHLLRLPAEGGEAKPATVLDSSRKDTAHRFPSFLPDG
                     DHFLYAALPPGPDGYAIFAGSLSSTKVTKVMVADSAPAYVEPGFLLFSRDGKILAQRF
                     DRGKLAVEGDAVPIAAAGNTGGMDTEPVASGATNGALVYLQAATANIQFQWFDRRGTV
                     TGSLPIAEGAWAFWDLSLDDRRVVIEKDGDLWAVDVERPVPTRLTSTGNNRAPVWTPD
                     GNRVAFYSNRAGSQEIYTVDAGGGDVVLVPTTTDKFKTPLDWSRDGRYLVFGSLGAST
                     DWDIWLLPLFGDKTPVPFLRSPFFDLNARVSPDGRWIAYESSESGQTEVYVQSFPTPG
                     RKARVSTTGGRDPAWSRGGRELIYRSGSNLVSVDFDENGGLGSAAPQVLFAMPFGTLG
                     GRASADGNRFLIGVPARDGQRPEICLIQNWTGLVGQ"
     gene            4703..>6567
                     /locus_tag="VF720_16340"
     CDS             4703..>6567
                     /locus_tag="VF720_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880981.1"
                     /translation="MRTEPCRSRSRSLTGRLGFVFAVLMLHLFTVRPLQAQVCGPLDL
                     NAPETAGGCPGTGVAFAFTVENQGSSNVDVYLRTSTSGGWVISPNVINDLAPNSPVQV
                     MATRSTTPSPSQILTLRASSYLSSTTPADTAGNSCDFVTETVSIVPGQTPCVTVSNPA
                     PIEACAGDTVDVAFTVTNCGPVAFNVNMAAACNGQNVSTVEPFNFNLNPGQSQVSTVT
                     CVVPEGSDPGDFLSIVNSAWAKTSTGCAFDVAGDTRVNTVTCPVSPSCGPLSLVGPPA
                     DTSCPNVPLSVTFTVENQGEPNVDVYLRTSSTGWTLNPTVVNNVASGGTQQVTATRND
                     PSASPQQLVVRATSYLATSTPADTAGESCDFRTETVNIVPGQQPCVTVSDPAPINGCA
                     GDTVDVVFTVTNCGSVAVNVNMVAACNGQTVSTVEPFNFNLNPGQSRISTVTCVVPDD
                     ADEGEYLTVVNSAWASTAAGCAFDIAGDTRINIGDCPVSPSCGPLSFVGPPADTSCPG
                     ESNSVTFTVENQGEPDVDVYFRTSSTGWSLNTTVINNLENGDVRQVIATRISPSNTTQ
                     QLVVRATSYLATSSPADTSGESCDFVRETVTIVPGLVPCGAPSCGPLSFFGPSADT"
BASE COUNT         1120 a         2233 c         2092 g         1122 t
ORIGIN      
        1 tgagctcccg ccgttcaagg ccgccatcga cggcggcgtg caggtggtga tgaccggtca
       61 ccttgcctgt cccgccctcg acccgagcgg ggctcccgcc acgctgagtc ctcgcatcct
      121 gagcggactt ctccgggacg aactcggctt cgaaggaatc atcgtctccg acgacatgga
      181 gatgtcgggc gtggccgcgc tgggggacgc tggcgccgtg gcaagaaacg ccatcgaagc
      241 cggcgtcgac atgctcctgt acgcccggag ccgggacatg gccgaagagg tccgcgagtc
      301 cctggccgat gccatcgagt ccggagaaat cgacgagcac cgggtgctcc aatcgctggc
      361 ccgccttggc cgcctgcgcg aggcgatccg cgccactcga accgagatcg atgacacggc
      421 gcgcgaggag gtagtcgatc atgaacacga ggaactcctg cgcgccgtcg ccatccaagg
      481 cgtcaccagc ctgggccccg tcggccccga gctaccggtc ccctggcccg atcgctacgg
      541 cctcatcatc attcccaccg ccgacgctcc ccggctcgac gtggaagtgg aatacctccg
      601 caacctcatc gagccactcc gcatcggcct cgaggtcgta agcctcaggc ccaccaccga
      661 acaacgcgaa gccgcccgct accgcgccag ccgcgccgat ttcctggtgg cctgcaccct
      721 cacgcgcggc gccatcgtgc ccgaacaacg ccagctcatc gacgacctgc tgatcctcaa
      781 gaaacaactc acggtcgttt cgctgctcga cccgcacgac ctgtccgaat accccaccgt
      841 ccgcacgcgc ctcgccacga gaggcttcca ccgccacgcc ctcgacgcgc tggtccatat
      901 cctcaccgga ctcagcgagc cccggctcga tcccgccagt caccaaattc cttgactgtc
      961 gttccgcccc gtggtaattt cccccgcctg aaaacgacca acaaaggcgg ggtcgtagtt
     1021 cagttggtta gaacgccggc ctgtcacgcc ggaggtcgcg agttcgagtc tcgtcggccc
     1081 cgccagttaa cttgagaaag ccccctcagc tcttcggttg agggggcttt cttgtttgca
     1141 gaaatgcagt tagcgcggcc tgcgatcgcc cgcgtcccat cccccgtccg gaagtgccaa
     1201 gtcagccgcg agagggggat aatcgccacg ccaagtgtgg tcttcacaga aacctgatcg
     1261 ggggggatct cggatcgcac accgccagga ggctcgttcg aaagtgggcg gcactcatga
     1321 cccagattcc gatagttgtc cgcgccgagt accggagtgc atttcggatc catctcacct
     1381 tcaatgatgg aaccgcgaaa accgtcgact tcaacgattg gcttgagggg ccgactttcg
     1441 aggcgctcaa gagcgaggac tacttcaagg aattcttcct cgacggcggc acagttgcct
     1501 ggtcgaatgg ggctgacatc gcacccgaga cgctctacga ggcaaggtcg gcggactagg
     1561 ccgcttgacg ttacgggagt gcgctttcga aaggccgagc gagctgtcca ggcagccgag
     1621 actctcctgg gcaaggggga cgcctagttt tccgccggta cgggaggctt cctgacgtaa
     1681 tatcccgcga tgccgctgac cgccgggacc cgcctcggac cctacgaagt cgtgtcgccc
     1741 cttggctccg gcggcatggg cgaggtgtat cgggcgcgtg acacgcgcct cctgcgcgac
     1801 gtggcggtca aggtgctgcc ggagcagttg tccgccaatc ccgacctgcg ggctcgattc
     1861 gagcgcgagg cacgggccat ctccagcctc aaccatcctc atatctgcac gcttcatgat
     1921 gtcgggcgcg aaggtggcat cgattacctg gtgatggaga tggtggaggg cgaaacgctc
     1981 gccgcgaggc tggagcgcgg cccgctcccg cttcccgagg tgttgcggac cggcgcccag
     2041 attgctgacg cgctggatcg ggcacatcgt gccggagtgg ttcaccggga tctgaagccc
     2101 gggaacgtca tgctcacgaa gagcggcgcc aagctcatgg acttcggact ggcgcgggcc
     2161 accggcctgg ccgggcccat gggggagggc gggcggaaca cggcactgac acagtcgccc
     2221 acgctcgtgc agccgcttac ggccgaaggt tcgatcatcg gcacgttcca gtacatgtcg
     2281 ccggagcagc tggaggggaa agaggccgat gcccgcagcg acctgtgggc gctggggtgc
     2341 gtgctctacg agatggcaac ggggaggcgg gcgttcgacg gccggagcca ggcaagcctc
     2401 atctcttcga tcatgaaaga ggagccggcg ccgatcggca caacgaatcc ggtgcacaca
     2461 ggcaccgccg gcttcggtct cgagcgcctg gtgagtcgat gcctggccaa gagtcaggac
     2521 gatcgctggc agaacgcggg cgacctgaag cacgagctgg agtggctcgc cagcggccag
     2581 tcgagcctcg gtgcgtcggt agtggcggag gccgcgcgtg cgcccgccac ccggcgcagg
     2641 cggagcgaaa cgttggcctg ggtgctggcc gccgccggaa tcggcaccgc cctggttctc
     2701 tacacgcgca tggagcgctc ctcgccgggc gctggagtca tggcgcgcgt gtcgctcccc
     2761 cttacccagc atctccagct ctcgagcgag agctgcgaca tcgccatttc tccggacggc
     2821 cgcaccgtgg ccttcgtcgc ggccgattcc accgatgtcg atcggctctg gctccgatct
     2881 ctggactcac cgacggccag gatgatcccc gagagcgaaa gcgccacgtc tcccttctgg
     2941 tcccccgaca gtcggcaggt ggcttttttc ggggagggct cgcgcaagct caagaaggtg
     3001 tccgcagcgg gtggctcgcc cgtggtgctg gccgatgcct cgacgccgcg tggcggatcc
     3061 tggagctcga aaggcgtgat cgtcttcgcg cccgccccgg aaggccacct gctccgcctg
     3121 cctgcggagg gtggtgaggc gaaaccggcc accgtgcttg actcctcacg gaaggacacg
     3181 gcgcatcgat ttcccagctt cctaccggac ggggaccact ttctgtatgc ggccctccct
     3241 cccggcccgg acggctacgc catctttgcc gggtcgttga gctcgaccaa ggtgacgaaa
     3301 gtgatggtgg ccgattccgc cccggcatac gtcgagccag ggttcctcct cttttcccgc
     3361 gatggaaaaa tacttgccca gcggttcgac cggggaaagt tggcagtcga gggcgatgcc
     3421 gtcccgatcg cggcagcggg caacaccggc ggaatggaca ccgaacccgt ggcttcggga
     3481 gcgacgaatg gtgcgctggt ctatctccag gccgccaccg cgaacatcca gttccagtgg
     3541 ttcgatcgac ggggaaccgt gacgggatcg ctgccgatcg ccgaaggtgc ctgggctttc
     3601 tgggacctct cgctcgatga tcggcgcgtg gtcatcgaga aggacgggga cctctgggcg
     3661 gtggacgtgg agcggccggt gccgacgcga cttacctcga ccggcaacaa tcgggccccg
     3721 gtctggactc ccgacggaaa ccgcgtcgcg ttctactcga accgggccgg atcccaggag
     3781 atctacaccg tcgacgcggg tggcggggat gtcgtgctgg ttccgacgac gaccgacaag
     3841 ttcaagacac cgctggactg gtcgcgcgac ggccgttacc tcgtcttcgg aagcctcggg
     3901 gcttcgaccg actgggacat ctggcttctt cccctgttcg gcgacaagac gccggtcccc
     3961 tttctccggt cgccgttctt cgatctcaat gcccgcgtct ccccggatgg ccgatggatc
     4021 gcctacgagt cgagcgagtc gggccagacc gaggtgtacg ttcaatcctt tccgactccg
     4081 ggccgcaagg cgcgcgtgtc gaccaccggc ggccgggacc cggcctggtc acgcgggggc
     4141 cgcgagttga tctatcgatc gggctccaac ctggtgtccg tcgacttcga cgagaacggc
     4201 ggcctcggat cggcagctcc ccaggtcttg ttcgccatgc cgttcggaac actggggggg
     4261 cgggcatcgg ccgacggcaa ccggttcctc atcggcgtgc cggcccggga tgggcagcgg
     4321 ccggagatct gcctgattca gaactggacg ggattggtcg gacagtaact ccggcgcgca
     4381 ggaagtgcgt caaggaaccc ggatagcggt agagcacttt gttcacactg atccggcgca
     4441 gcgaacatag ttcctgaatc agcacctcgc ttcctggcga caatcccacg ccgagcgtgg
     4501 tcgatcatcg cagtgcccca atcccccatg gggtcattcc gcaatcgcat ctacgtcact
     4561 cacttttgct tgtatccccc cggaagcggt ccctagtctc gacaatggtc gcgacatttc
     4621 ccggggacgc acggccgcat gtctcccgca tcgagcacga catccaacac tgaatcaatg
     4681 gctgagccat tggggggatc acatgcgcac cgaaccttgc cgttcccggt ccaggtcact
     4741 gacaggccga ctgggcttcg tcttcgcggt attgatgctt cacctcttca ccgtacgccc
     4801 tttgcaggcg caggtctgcg gcccgctgga cttgaacgca ccggagacgg caggcgggtg
     4861 ccccggtacc ggtgtggcgt ttgcgttcac ggtcgagaac cagggttcct ccaatgtgga
     4921 cgtgtacctt cggacgtcca ccagcggtgg gtgggtcatc tcgccgaatg tcatcaatga
     4981 cctggcaccg aactctcccg tccaggtcat ggcgacgcgc tccacgacgc cgtccccctc
     5041 gcagatcctg acgctgcggg cttcgtcgta cctgtccagc accacgcctg ccgacacggc
     5101 gggaaacagc tgcgatttcg tgacggaaac ggtcagcatc gtgcccggcc agacgccgtg
     5161 cgtgaccgtg agcaatccgg cgccgatcga ggcctgcgcg ggcgacacgg ttgacgtggc
     5221 cttcaccgtc accaattgcg gacccgtcgc cttcaacgtg aacatggcgg cggcctgcaa
     5281 cggccagaac gtgagcacgg tcgagccgtt caacttcaac ctcaatccgg gccagtccca
     5341 ggtgagcacg gtgacgtgcg tggtgcccga gggctccgat ccgggcgact tcctgagcat
     5401 cgtgaacagc gcgtgggcca agaccagcac cgggtgcgcg ttcgacgtgg ccggcgacac
     5461 ccgcgtcaac accgtcacct gtccggtgag tccgtcgtgc ggaccgctct cgctggtggg
     5521 gccgccggcc gataccagct gcccgaacgt gccgctctcg gtcaccttca ccgtagagaa
     5581 ccagggtgag ccgaatgtgg acgtctatct ccgcacctcg agcaccggct ggacgctgaa
     5641 ccccaccgtc gtcaacaacg tggcttcggg cggcacccag caggtcaccg ccactcgcaa
     5701 cgatccgtcg gcttcaccgc agcagctcgt ggtccgcgcc acctcgtacc tggctacgtc
     5761 gacaccggcg gatacggccg gcgagtcgtg cgacttcaga accgaaaccg tcaatatcgt
     5821 gcccggccag cagccgtgtg tgaccgtgag cgacccggcc ccgatcaatg gctgcgccgg
     5881 cgacacggtg gacgtggtgt tcaccgtcac caactgcggc agcgtggccg tcaacgtgaa
     5941 catggtggcg gcgtgcaacg gccagaccgt gagcaccgtc gaacccttca acttcaacct
     6001 gaatcccggc cagtcccgca tcagcacggt gacgtgcgtg gtgccggatg atgccgatga
     6061 gggcgagtac ctgacggtcg tcaacagcgc atgggccagc acggccgccg gttgcgcgtt
     6121 cgacatcgcc ggcgacaccc ggatcaacat cggcgactgc ccggtgagtc cgtcgtgcgg
     6181 cccgctgtcg ttcgtcggcc cgccggccga caccagctgc cccggcgaat cgaactcggt
     6241 caccttcact gtcgagaacc agggcgaacc ggatgtcgat gtctatttcc gcacgtcgtc
     6301 gaccggctgg agcctgaata ccaccgtcat caacaacctt gagaacggtg atgtccggca
     6361 ggtgatcgcc acgcgtatca gcccgtcgaa caccacgcag cagctcgtgg tgcgcgccac
     6421 ctcctacctg gccacgtcgt ccccggccga cacgagcggc gagtcgtgcg atttcgttcg
     6481 cgagacggtg accatcgtgc ccggcctggt gccgtgcggt gcgccgtcgt gcgggcccct
     6541 ctcgttcttc ggcccatcgg ccgatac
//
LOCUS       DASVOD010000762         3596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_623556, whole genome shotgun sequence.
ACCESSION   DASVOD010000762 DASVOD010000000
VERSION     DASVOD010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623556"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..568)
                     /locus_tag="VF720_16345"
     CDS             complement(11..568)
                     /locus_tag="VF720_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880982.1"
                     /translation="MRRTTLSMAAITLLSIALHLPSVAATNIDAPEISRSRGTKGSIV
                     VLWPRIIPAWGGSETPIMARAVQEKLVALAREAYPGRPIDVRPSPERVCPMGGCDGLA
                     VGALLTRDGEGCAVVALVSPGGTSPTTMVPWAGSVRLRAGRVPFRQPPENWVTITDFG
                     RCDQMGADLEINRASIVAAIRELGR"
     gene            complement(668..1723)
                     /locus_tag="VF720_16350"
     CDS             complement(668..1723)
                     /locus_tag="VF720_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880983.1"
                     /translation="MRFGSRTTDPLRGLASLGVVLGLLVTIPGTAGAWGYAVHRLINR
                     NAVANLPSDFSGFAQYAAQLEALATAADERKSSTPGENIRHYINIDEFAEFQAGTFPH
                     QYADAVSRYGLSRLQSAGVGPWALENSYNQMVAAFVARDWTAAVAAAGDIGHYAGDLH
                     QPLHLTSNYDGQESGQRGLHSRFESRLTGKHMTDFTPFPGAASFVADPLEREFAWINT
                     TYPGVSVILAADLAAKSAAGGSTSSDTYYNSMWEQLGQDTWYWIGLASFDIASFWYSA
                     WIEAGAPPLPGTSPVEPATWSRIKGLLVAGGATRPVRQPIASPAGSSRTNGRNRPGPV
                     INSLPTGSLVSHHDCGE"
     gene            1846..2277
                     /locus_tag="VF720_16355"
     CDS             1846..2277
                     /locus_tag="VF720_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880984.1"
                     /translation="MTPPPFHQRHRGPILAAFLITAAAVLFMADPVRRALLPASAVIV
                     EFNHPAATSTARVRIVEDRGPVRIGHADGSPVEEIVLDGGRIAIDVKGEPRNFKIDIP
                     RATPFMELRVSDRTVFQRKGSVVTTIWPIDSDSTWLVPLTP"
     gene            complement(2302..>3596)
                     /locus_tag="VF720_16360"
     CDS             complement(2302..>3596)
                     /locus_tag="VF720_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX7880985.1"
                     /translation="EGTYGWIPAETLYGPTIYSPRDKTTRFAVGAGLQHVGADVILVL
                     PLGRIAPYALGGYQRLIYDSDQESAPEADDDISGPELALGLIGYVNPNLALRAEVRDV
                     SHSFDQADALDDARQDNIFFTIGIQYGFGGHVESHDQDNDTVSDDIDACPNTPFGARV
                     DATGCPIDSDGDGVPDGLDTCEHTPRGATVNAAGCPSDADGDGVPDGIDKCPDTAAGL
                     KVDATGCTMDSDHDGVADGTDQCANTPTGARVDAVGCPLDEDKDGVPDGLDKCPGTPA
                     SSRVDKDGCEIKVSEKETELLDTGKITIRDINFETAKWTILPDSYKVLEEVGSILVQW
                     PELRVEIGGHADSRGSDEYNLNLSQKRAQAVLDYLIGKFPQITAAQYTARGYGESQPV
                     APNTTVEGMARNRRVEFKVLNTEILKKERESQKTLKKE"
BASE COUNT          534 a         1182 c         1231 g          649 t
ORIGIN      
        1 cctcccgtcg tcagcggccc agttccctta tcgcggccac gatcgaagcg cgattgattt
       61 cgaggtcggc ccccatctgg tcgcatcggc cgaagtcggt aatcgtcacc cagttctcgg
      121 gtggctggcg aaaaggaacc cgtcccgcgc ggagtcgcac gcttcccgcc cacgggacca
      181 tggtggtcgg cgacgtgccg cccgggctga cgagggccac caccgcgcaa ccttccccat
      241 cccgcgtcag cagggccccg acggccaggc cgtcacagcc tcccatcggg cagacccgct
      301 cgggtgaggg gcgaacgtcg atcggacgac cgggatacgc ttcgcgcgcg agcgccacca
      361 gcttttcctg cacggcccgg gccatgatgg gagtttcgga gccgccccag gccggaatga
      421 tgcgcggcca gaggaccacg atgctcccct tcgtgccgcg cgagcgcgag atctcggggg
      481 cgtcgatgtt ggtggcggca accgacggaa gatgaagtgc gatcgacaac agggtgatcg
      541 cggccatgga aagtgtcgtc cgtctcatgg tgcagtttcc tcctgcccgg aattttcgca
      601 agtgacatgc catcctggag cgcatcgtct caatcgaaag tgtagatgga ggttgggccg
      661 cggtccgcta ttccccgcag tcgtggtgag acacgagcga accggtcggc agggagttaa
      721 ttactggccc gggccggttt cgtccgttgg tgcgggagga gccagcggga gaggcaatcg
      781 gctggcgaac gggccgcgtt gcgccgccgg ccacgaggag ccccttgatc cggctccagg
      841 tggccggctc gaccgggctc gtcccgggga gggggggcgc gcccgcttcg atccaggccg
      901 agtaccagaa ggaggcaatg tcgaacgacg ccagaccgat ccagtaccag gtgtcctgcc
      961 ccagctgttc ccacatcgag ttgtagtagg tgtcgctcga cgtgctgccg ccggcggcgg
     1021 acttggccgc caggtccgca gccaggatca ccgacacccc cgggtaggtg gtgttgatcc
     1081 aggcgaactc ccgctcgagc gggtcggcca cgaaggatgc cgcaccgggg aagggggtga
     1141 agtcggtcat gtgcttcccg gtcaggcgcg actcgaaccg tgagtgcagg ccgcgctggc
     1201 ccgattcctg gccatcgtag ttgctggtca ggtggagcgg ctggtggagg tcgccggcat
     1261 agtgcccgat gtctccggcg gcggccacgg ctgctgtcca atcccgcgcc acgaaggccg
     1321 ccaccatctg gttgtagctg ttttccagcg cccatggacc cacgccggcc gactgcagcc
     1381 gcgacaggcc gtaccgcgag accgcatccg cgtactggtg cggaaaggtg ccggcctgaa
     1441 attcggcgaa ctcgtcgatg ttgatgtagt ggcggatgtt ttcaccgggc gtgctcgatt
     1501 tccgctcgtc cgcggcggtg gcgagggcct ccagctgggc cgcgtactgt gcgaatcccg
     1561 agaagtcgga tggcaggttg gccaccgcgt tccggttgat caggcggtga accgcgtagc
     1621 cccaggcgcc ggccgtgccg ggaatcgtga ccaggaggcc gagcaccacc ccgagcgagg
     1681 cgaggcccct gaggggatcg gtggtgcgtg agccaaagcg catccgggga gtgtatcgca
     1741 gcacgacccg tttcggcccc gccaatctgg agtctccgtc tggcggcgct gcgatgctgg
     1801 agtctccgtc tggcggccgt gcgatggagg gtgtagcgtg tcgccgtgac gcctcctccc
     1861 ttccaccagc ggcatcgggg cccgatcctg gcggcctttc tcattaccgc cgccgcggtt
     1921 ctattcatgg cggatccggt ccggcgcgcg ttgctgccgg cgtcggccgt tatcgtggag
     1981 ttcaatcatc cggccgccac ctcgaccgcc cgtgtgcgga tcgtggagga ccggggcccg
     2041 gtccgcatcg ggcatgccga cggatccccg gtggaggaaa tcgtcctgga tggtggacgc
     2101 atcgccatcg acgtgaaggg cgagccccgg aatttcaaga tcgacatccc gcgggccacg
     2161 cccttcatgg aattgcgcgt cagcgaccgg accgtctttc aacgcaaggg gtcggtcgtg
     2221 accaccatct ggccgatcga cagcgactcg acctggctgg tgcccctcac gccctgagtc
     2281 cgggcacgac ttcgtccgtc attactcctt cttcagggtc ttctggctct cgcgctcctt
     2341 cttcaggatt tccgtgttga gcaccttgaa ctccacgcgg cgattgcggg ccataccctc
     2401 gaccgtggtg ttgggggcca ccggctggct ctccccgtac cccctggcgg tgtactgggc
     2461 cgcggtgatc tgcgggaact tgccgatcag gtagtccagg accgcctgcg cgcgcttctg
     2521 ggacaggttc aggttgtact cgtcggagcc gcgactgtcg gcatgcccgc cgatctccac
     2581 gcggagttcc ggccattgca cgagaatgga gccgacctcc tcgagcacct tgtacgagtc
     2641 cggaaggatg gtccacttgg ccgtctcgaa attgatgtcg cggatggtga tcttgccggt
     2701 gtcgagaagc tcggtttcct tctcgctcac cttgatctcg caaccgtcct tgtccacgcg
     2761 tgagctggcc ggtgtgccgg ggcacttgtc caggccgtcg ggtacgccgt ccttgtcctc
     2821 gtcgagaggg caaccgacgg cgtccacgcg ggcgccggtc ggcgtgttgg cacactggtc
     2881 ggttccgtcg gccacgccgt cgtggtcgga gtccatcgtg cagccggtgg catccacctt
     2941 gaggccggcc gccgtatcgg ggcacttgtc gatgccgtcc ggcaccccat ccccgtcggc
     3001 gtcgctgggg caaccggcgg cgttcaccgt ggcgccgcgc ggcgtgtgct cacaggtgtc
     3061 gaggccgtcc ggcacgccgt caccgtcgct gtcgatcggg cagccggtgg catcgacgcg
     3121 ggccccgaac ggcgtgttgg ggcaggcatc gatgtcgtcc gacaccgtgt cgttgtcctg
     3181 gtcatgcgat tcgacgtggc cgccgaaccc gtactggatg ccgatcgtga agaagatgtt
     3241 gtcctgtcgg gcgtcgtcca acgcgtcggc ctggtcgaac gagtggctca cgtcacgcac
     3301 ttcggcacgg agcgccaggt tcggattcac gtagccaatg aggcccagag ccagctcggg
     3361 cccggagatg tcgtcatccg cctcgggcgc ggactcctgg tccgagtcgt agatgagccg
     3421 ctggtatccg cccagcgcgt aaggcgcaat cctgccgagc ggaagaacca ggatcacgtc
     3481 cgcgccgacg tgctggaggc cggcgcccac cgcgaaccgg gtcgtcttgt cacgggggct
     3541 gtagatcgtc gggccgtaaa gcgtttcggc cggaatccag ccgtacgttc cctcca
//
LOCUS       DASVOD010000763         2004 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_520281, whole genome shotgun sequence.
ACCESSION   DASVOD010000763 DASVOD010000000
VERSION     DASVOD010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520281"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..>2004
                     /locus_tag="VF720_16365"
     CDS             588..>2004
                     /locus_tag="VF720_16365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX7880986.1"
                     /translation="MSPLTPGSRLGAYDIVGPLGAGGMGEVYRARDARLGRDIAVKVL
                     PSDTARHPDRLARFEREARTVAAMNHPNIVTIHSIEQDGDVRFLTMELVDGDSLDHQV
                     TPGGLAVARLLGLAVPLADALVAAHERGVVHRDLKPANVMVTRAGRVKVLDFGLAKGV
                     DPASDIDSTQAATLASPISTAGQVVGTVPYMAPEQVRGEPADARTDLFALGVLLYELL
                     VGTRPFTGKSFADVSSAILRDQPAPVSTRRPDAPADLARIIMRCLEKDPRHRFQTALD
                     VYNELQLVKRAENPSTTSASSSAVKAVASPAAADPPSIAVLPFVSRGRDEEDEYFADG
                     LADELMSVLARIRGLRVAARTSTFSFRGKDATIAEIGRALNVGTILEGSIRKSGPRMR
                     ISVQLVNVADGYHLWTETYDRTLEDIFAVQDEIAQAVVKELRTVLHGGEPDSKTSGEV
                     KAEVAEAVRGRTADAQAHHDFV"
BASE COUNT          319 a          680 c          684 g          321 t
ORIGIN      
        1 aggatgctct ccactctgtg ccgttccgcc gcagcgctgg aatccatcca ttaatggggg
       61 atcagccgac agcaccggtg gcggtcactg cagagaacat cgtctcgttc tctttcaggc
      121 ccgaccgact cgcgtcaccc ggacggtatg cacgtaatgc aggcggcgtg ccacgcggcg
      181 gtgggtgccg ccagctgacc tcgatgcatg cgggcgcagg cgatcattga gtcggcaact
      241 gatgatgaat gtgtgacttg cggaggaaca tcgttgtcgt tcgcctggat gccgggggac
      301 ggcacaaccg tcagccaggc gccttcaccc gtgtctggga gtccgttatc gggggattcg
      361 acgcaacgat ttcgggggag atgccgcacg cgggtgggtg agccgggtgg ggagcttggt
      421 tagaagatat tccgggtgtc ttactttgta agacacgtcg tgaatcgacc aaccgaaggc
      481 gggccgctgg ccgccccagg ccctcgggcg cctctctcgt ccccgcccct ctgggacgtt
      541 ttcgcacacc tgcctcccca acccaaagct gctagcattc gccgcccatg agcccactga
      601 ctcccggcag ccgtctcggc gcgtacgaca tcgtcggtcc cctcggtgcc ggcggcatgg
      661 gggaggtata ccgggcccgc gacgcccgac tcggtcgcga catcgccgtc aaggtgcttc
      721 catccgacac cgcgcgccat cccgaccgcc tggcccgctt tgagcgcgag gcgcgcaccg
      781 tggcggccat gaatcacccc aacatcgtga cgatccactc gatcgagcag gacggcgacg
      841 tgcgcttcct gacgatggag ctggtggatg gcgacagtct cgatcatcag gtgactcccg
      901 ggggcctcgc ggtggcccgc ctcctcgggt tggcggtgcc gctggcggat gcgctggtgg
      961 ccgcgcatga gcggggcgtg gtccatcgcg acctgaagcc ggccaacgtc atggtcacgc
     1021 gcgccgggcg cgtgaaggtg ctcgacttcg gcctcgccaa gggggtcgac ccggcatcgg
     1081 atatcgactc gacccaggcc gccacgctgg cctcgccgat ttccaccgcc gggcaggtgg
     1141 tgggaacggt gccgtacatg gcgccggagc aggtgcgcgg cgagccggcc gacgcccgca
     1201 ccgacctgtt cgcactgggc gtcctcctgt acgaactcct ggtcggtacg cgcccgttca
     1261 ccggcaagtc gtttgccgat gtcagctcgg ccatcctgcg ggaccaaccg gcaccggtga
     1321 gcacgcgacg gcccgacgcc ccggccgacc tggctcgcat catcatgcgc tgcctcgaga
     1381 aggacccgcg gcatcgcttc cagacggcgc tcgacgtgta caacgagctg cagctcgtga
     1441 agcgcgccga aaacccgtca acgacgtcgg cctcgtcgtc ggccgtgaag gccgtcgcgt
     1501 cgcccgccgc cgccgacccg ccctcgatcg cggtgctgcc gttcgtgagt cggggccgcg
     1561 acgaggagga cgagtacttt gccgacggcc tggccgacga actgatgagc gtgctcgcca
     1621 ggatccgggg gctgcgcgtg gcggcgcgca ccagcacctt cagcttccgc ggcaaggacg
     1681 ccaccatcgc cgagatcggc cgcgcgctga acgtcggcac catcctcgag ggaagcatcc
     1741 gcaagtcggg tccccggatg cggatctcgg tgcagctggt caacgtggcc gacggctatc
     1801 acctctggac cgagacgtac gaccgcacgc tggaggacat cttcgccgtg caggatgaaa
     1861 tcgcgcaggc ggtggtgaag gagctgcgca ccgtgttgca cggtggcgag ccggattcga
     1921 agaccagcgg agaggtaaag gccgaggtgg ctgaggccgt gcgcgggcgg accgccgacg
     1981 cgcaggccca ccacgacttc gtgc
//
LOCUS       DASVOD010000764         3021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_675605, whole genome shotgun sequence.
ACCESSION   DASVOD010000764 DASVOD010000000
VERSION     DASVOD010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675605"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VF720_16370"
     CDS             complement(<1..572)
                     /locus_tag="VF720_16370"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEX7880987.1"
                     /translation="MESLTLRALKHEIDSRLPLALLRVAPAPDGDGLALVTGPLILIV
                     DAGGRRPPIRFLPAPEPGPRGEKNRAFEWTGNASWHEVAWHLGTRAAGGRITAVHGHG
                     LDRVLGITIARKDRFGDARQVTLVVEIFGRFVNVLLLDGGFDGAILARLRDDNRGVAG
                     ARIERGRHYEPPPRDRIDLELDGEAALVER"
     gene            601..1482
                     /locus_tag="VF720_16375"
     CDS             601..1482
                     /locus_tag="VF720_16375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEX7880988.1"
                     /translation="MIRSMTGFGRAELDRNGLALSAEIRTVNHKFCEVSVRLPRSLSN
                     LENRVRLKIQETLTRGKVNMTVGWKDGREQEGELVVDRALAGQYVKALTELRQEFKFV
                     DAIDLKTLVGLPDVFRWREPTIEEALAIETIDEITVRSLADLLRMRDEEGQTLRRDLE
                     ARIDTILRRLEVIVERSPVRVNELKEKLRARIAQLLAGEAQVPDEKIVVEASFLADRL
                     DCTEEAVRLRSHCDQFLKLAAAPEPAGRKLNFLVQEMNREVNTIGSKSNDVDIAREVI
                     VLKEEIEVIREQVQNIE"
     gene            1486..2100
                     /gene="gmk"
                     /locus_tag="VF720_16380"
     CDS             1486..2100
                     /gene="gmk"
                     /locus_tag="VF720_16380"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX7880989.1"
                     /translation="MTPLDPLDTRPGTAVFPLVISGPSGVGKSVLIEKLIARERWLVC
                     SVSNTSRAPRAYEKDGVHYHFRSNDEFERAIQANGFLEWARVHDHYYGTPKAPLEAHL
                     AAGRGVVMNIDVQGGLAIRAARPDAVLIFILPPSMAVLEKRLRQRKSDSEEAIQRRLT
                     NARGEIEKAGAYDYLVVNDDLETTVVELAAIIGAERHRTSRLMG"
     gene            2298..2519
                     /locus_tag="VF720_16385"
     CDS             2298..2519
                     /locus_tag="VF720_16385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7880990.1"
                     /translation="MPENKPAFEHKLNKYEMVVVAAREARRLNEMAHQQGRELKGRVT
                     DLAMKRFLKGDVKYRYEETPAPPVQPPTI"
     gene            2551..>3021
                     /locus_tag="VF720_16390"
     CDS             2551..>3021
                     /locus_tag="VF720_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018874047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEX7880991.1"
                     /translation="MNDKPRIVLGVSGGIAAYKSAELVRLLIKDGMDVRVVMSRAATQ
                     FVTPLTLGTLSGHPVLVHLFESDAAPGTYAPDEISPGGVDSGIEHIDIIKEADLVVVA
                     PATADLLAKAAHGLGDDALTTVLLAAGPRVHFAPAMNHRMWNHPATQMNLATLTS"
BASE COUNT          537 a         1048 c          965 g          471 t
ORIGIN      
        1 aatcgctcga cgagcgccgc ctcgccgtcg agttcgagat cgatccggtc gcgcgggggg
       61 ggctcgtagt gccgtccccg ttcgatgcgc gcgccggcca ccccgcgatt gtcgtcgcgg
      121 agccgggcga gaatggcgcc gtcgaagccg ccatccagga ggagcacgtt cacgaaacgg
      181 ccgaagatct ccacgaccag tgtcacttgt cgcgcatcgc cgaaacgatc cttccgggca
      241 atcgtgatcc cgagcacccg gtcgagcccg tgcccgtgca cggcggtgat ccttcctcct
      301 gcggctcggg tgccgaggtg ccacgccacc tcgtgccagg aagcgttgcc ggtccattcg
      361 aacgcgcggt tcttctcgcc ccgcggaccg ggttccggcg ccggaagaaa gcggatcggc
      421 ggccggcggc cgccggcatc cacgatgagg atgagcgggc ccgtcaccag cgccagcccg
      481 tcgccgtccg gcgcgggtgc cacgcgcagg agcgccaggg gaagccggga gtcgatctcg
      541 tgcttcagcg cacgcagggt cagcgattcc atgggccgcg tatgatatcg tgcctcccct
      601 atgatccgca gcatgaccgg ctttggccgg gccgaactcg atcgcaacgg ccttgccctc
      661 tcggccgaaa tccgcacggt caaccacaag ttctgtgaag tgtccgtgcg cctgccgcgc
      721 tccctttcca acctcgagaa ccgcgtgcgc ctcaagatcc aggagacgct gacccgcggc
      781 aaggtgaaca tgaccgtggg ctggaaggac ggccgcgagc aggaagggga gctggtcgtc
      841 gatcgcgcgc tggccgggca gtacgtgaag gcgctcacgg aactgcgtca ggaattcaag
      901 ttcgtcgatg ccatcgatct caagacgctg gttggtctgc ctgacgtctt ccgctggcgc
      961 gagcccacga tcgaggaagc cctggccatc gagacgatcg acgagatcac ggtgcgcagc
     1021 ctggccgacc tcctccgcat gcgcgacgag gaagggcaga cgctccgccg cgacctcgag
     1081 gcccgcatcg acaccattct ccggcggctc gaggtcatcg tcgagcgctc gcccgtccgg
     1141 gtgaacgagc tcaaggagaa gctccgggcg cgcatcgccc agctcctggc cggcgaggcg
     1201 caggtgcccg acgagaagat cgtcgtcgaa gcctccttcc tggccgaccg tctcgactgc
     1261 accgaggaag ccgtccgcct ccgctcgcac tgcgaccagt tcctgaagct ggccgccgcg
     1321 cccgagcccg ccggccgcaa gctcaacttc ctggtccagg agatgaatcg cgaggtcaac
     1381 accatcggct ccaagtccaa cgacgtcgac atcgcccgcg aagtcatcgt cctgaaggag
     1441 gagatcgagg tgatccgcga acaggtccag aacatcgagt aggcgatgac tcccctggat
     1501 ccgctcgaca cccgccccgg caccgccgtc tttccgctcg tcatctccgg gccgtccggc
     1561 gtggggaaat cggtcctgat cgagaagctg attgcgcggg aacggtggct ggtctgttcc
     1621 gtgtcgaaca ccagccgcgc cccgcgggcc tacgagaagg acggcgtgca ttaccacttc
     1681 cgctccaacg acgaattcga gcgcgcgatc caggccaacg gcttcctcga gtgggcccgc
     1741 gtgcacgacc attactacgg cacgccgaag gcgcccctcg aagcccacct ggccgccggc
     1801 cgcggcgtcg tgatgaacat cgacgtccag gggggcctgg ccatccgcgc cgcccggccg
     1861 gacgccgtgc tcatcttcat cctcccgcca tcgatggccg tcctggaaaa acgtctccgc
     1921 cagcggaaga gcgattccga ggaagcgatc cagcgccgcc tcaccaatgc ccgcggcgag
     1981 atcgagaagg cgggggcgta cgactacctg gtggtgaacg acgacctgga gacgaccgtg
     2041 gttgagctgg cggcgatcat cggggcggaa aggcaccgga cgtcgcggtt gatgggctga
     2101 aaccgggccc tgcgcgttcc tggatcccaa agtcacttac tattctagtt agttaagatg
     2161 taagtttatt tccttggatt gtggcgaatt atcgtgcaaa acaggcatca agggaggccc
     2221 tgggaaaggc gctcgccctc gcccccgacc gtgctatcct ccttgccctc ttacctatta
     2281 ttaagaagga gcccactgtg cccgagaaca agccggcgtt cgaacacaag ctcaacaagt
     2341 acgagatggt cgtcgtggcg gcgcgggaag cccggcgcct gaacgagatg gcgcatcagc
     2401 aggggcgcga gctgaagggg cgggtcaccg acctggccat gaagcgcttc ctgaagggcg
     2461 acgtgaagta tcgttacgag gagacgccgg cgccgccggt ccagccgccg acgatctgat
     2521 ccgcggccac tctccgacgg gggtttcgcg gtgaacgaca agcctcgcat cgtgctcggc
     2581 gtgagcggcg gcatcgccgc ctacaaatcg gccgagctgg tgcgcctcct catcaaggac
     2641 gggatggacg tgcgcgtggt catgagccgc gccgccaccc agttcgtcac gcccctgacg
     2701 ctcggaacac tttccggcca cccggtgctc gtccacctct tcgaatcgga cgcggcgcct
     2761 ggcacctacg cccccgatga aatctcgccc ggcggcgtcg attcgggaat cgagcacatc
     2821 gacatcatca aggaggccga cctcgtcgtg gtggcgcccg ccactgccga cctgctggcg
     2881 aaggccgccc acggcctcgg cgacgacgcg ctcaccaccg tcctcctggc ggccggaccg
     2941 cgcgtccact tcgccccggc catgaaccac cgcatgtgga accacccggc cactcagatg
     3001 aacctggcca cgctcacgtc g
//
LOCUS       DASVOD010000765         2917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_727438, whole genome shotgun sequence.
ACCESSION   DASVOD010000765 DASVOD010000000
VERSION     DASVOD010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727438"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VF720_16395"
     CDS             complement(<1..869)
                     /locus_tag="VF720_16395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX7880992.1"
                     /translation="MKVSVFGLGYVGCVTAACFARDGHTVLGVDVSPAKVAEINAGRA
                     PFFEPGLDALTKEGVAAGRLTATGSAAEAIHTTDFGLICVGTPSLSDGAQNLDYVRRV
                     CQEIGESLARRESARFFTVIVRSTLLPGIFEEVILPVLEKASGKKAGVDFGAVQNPEF
                     LREGTSIADFDNPPFTVIGELNARSGEEAAKLYANLSAPLFRTSPGAAAMVKYACNAF
                     HAVKVSFANEIGNLCKSAGVDSHDVMKVFVEDTKLNLSSYYLKPGFAFGGSCLPKDIR
                     ALQAVARLNGLEVP"
     gene            complement(869..2581)
                     /locus_tag="VF720_16400"
     CDS             complement(869..2581)
                     /locus_tag="VF720_16400"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX7880993.1"
                     /translation="MSDILYIGFHKSHDSSLCLTTAEGEMLFVANEERFTRKKLQAGI
                     PFQCKEYLLKHYKVGPVVFCYGGLKVKDRLKREAAFHMASLAKGLNTPPLHHDIGKLY
                     GRLTGRNDNPNRPKLFDGFDIQEVVHVDHHDCHAASAYYQSGCDEAIVMTLDGLGDCF
                     SAAFYRGRGSKLERTRAYYHNDVTIGEDYEMMTALLGFNPHRHPGKITGLAAHGVYNE
                     ACIRAVRDFFKKVWKKGNRNFFYEFHTEKEEDAVAELRRLRDTMFGKFSREDMAFTIQ
                     YLCEEDTIALIKENVSEPANHNICLAGGVFANVKLNKRVHELGFRNVFVQPAMSDAGL
                     ATGAVLWHLGATKGLRPYALKNVYGGPGYPQAEIEAACKAEGLEYEVVNPIEPRIAQL
                     LAGGAVVARFEGRMEFGPRALGHRSILYAAKDPTVNDWLNKRLQRTEFMPFAPSTLWE
                     YADQSFERVNGAHHTAQFMTITFDCTPWFRETCPAAVHVDGTARPQLVRKDESPSYWT
                     LIDEYRKLTGVPTVINTSFNMHEEPIVCTPADAVRAFMDGHLDWLAMDNVLVRNPQLE
                     SKKA"
BASE COUNT          459 a          919 c          994 g          545 t
ORIGIN      
        1 gggacctcga ggccgttcag ccgggccacg gcctgcaggg cgcggatgtc cttcggcagg
       61 caggagccgc cgaaggcgaa ccccggcttc aggtagtacg acgacaggtt gagcttggtg
      121 tcttccacga agaccttcat cacgtcgtgg ctgtccacgc cggcggactt gcagaggttg
      181 ccgatctcgt tggcgaacga cacctttacc gcgtggaagg cgttgcaggc gtacttgacc
      241 atggccgccg cgccgggtga cgtgcggaaa agcggcgccg acaggttggc gtagagtttc
      301 gcggcttcct cgccgctcct ggcgttcagc tccccgatca cggtgaaggg cggattgtcg
      361 aagtcggcga tggaggttcc ttcgcgcagg aactcggggt tctgcacggc gccgaagtcc
      421 acgccggcct tctttccgga ggccttctcg agcacgggca ggatgacttc ctcgaagatg
      481 ccggggagga gcgtggaccg cacgatcacg gtgaagaaac gggcgctctc ccgcctggcc
      541 agcgattcgc cgatctcctg gcagacacgc cgcacatagt cgaggttctg ggcgccgtcg
      601 gaaaggctcg gcgtgccgac gcagatcagc ccgaagtcgg tggtgtggat ggcttccgcc
      661 gcgctgccgg tggcggtgag gcggccggcg gccacccctt ccttcgtgag ggcgtcgagg
      721 cccggctcga agaagggggc ccgcccggcg ttgatctcgg ccaccttggc ggggctcacg
      781 tccaccccga ggacggtgtg gccgtcccgc gcgaaacagg cagccgtgac acagccgacg
      841 tacccgaggc cgaagaccga aaccttcatc aggctttctt cgactcgagt tgcggattcc
      901 ggaccaggac gttgtccatg gccagccagt cgaggtgccc gtccatgaag gcccggacgg
      961 cgtcggccgg cgtgcagacg atcggctcct cgtgcatgtt gaacgaggtg ttgatgacgg
     1021 tcggcacgcc ggtcaacttg cggtactcgt cgatgagcgt ccagtacgag gggctctcgt
     1081 ccttccgcac cagctgcgga cgcgcggtgc cgtccacgtg aacggcggcc gggcaggtct
     1141 cgcggaacca gggggtgcag tcgaacgtga tcgtcatgaa ctgcgcggtg tgatgcgcac
     1201 cattcacccg ctcgaagctc tggtccgcgt actcccacag cgtgctcggc gcaaacggca
     1261 tgaactcggt gcgctggagc cgcttgttga gccagtcgtt gaccgtcggg tccttggccg
     1321 cgtacaggat cgagcgatgg ccgagcgccc gcgggccgaa ctccatgcgt ccttcgaagc
     1381 gcgccaccac ggccccgccg gccagcagct gcgcgatgcg cggctcgatc gggttcacga
     1441 cctcgtattc aagcccctcg gccttgcagg ccgcctcgat ttccgcctgc ggataaccgg
     1501 gtccgccgta cacgttcttc agcgcgtagg ggcgcagccc cttcgtggcg cccaggtgcc
     1561 agagcaccgc gccggtggcc aggccggcat cgctcatggc gggctgcacg aagacgttcc
     1621 tgaagccgag ctcatgcacg cgcttgttca gcttgacgtt ggcgaagacg ccccccgcca
     1681 ggcagatgtt gtggttggcc ggttcggaca cgttttcctt gatgagggcg atcgtgtctt
     1741 cctcgcacag gtactggatc gtgaacgcca tgtcctcgcg gctgaacttg ccgaacatcg
     1801 tgtcgcgcag gcgacggagc tcggccaccg cgtcttcttc cttctcggtg tggaattcgt
     1861 agaagaagtt gcgattcccc ttcttccaca ccttcttgaa gaagtcgcgc accgcgcgga
     1921 tgcacgcttc gttgtacacg ccgtgcgccg cgaggccggt gatcttgccg ggatggcggt
     1981 gcgggttgaa gccgaggagc gccgtcatca tctcgtagtc ttcgccgatg gtgacgtcgt
     2041 tgtggtagta cgcccgggtc cgctcgagct tgctgccgcg ccccctgtag aaggcggctg
     2101 agaagcaatc gcccaggccg tccagagtca tgacgatggc ctcgtcgcag ccactctggt
     2161 agtaagcgct ggcggcgtgg cagtcgtgat ggtccacgtg gacgacttcc tggatgtcga
     2221 atccgtcgaa gagcttcggc cggttcggat tgtcgttccg gccggtgagc cgcccgtaga
     2281 gctttccgat gtcgtggtgg agcggcgggg tgttgagccc cttggccagg ctggccatgt
     2341 ggaaggctgc ctcccgcttg agccggtcct tgaccttgag gccgccgtag cagaagacca
     2401 ccgggccgac cttgtagtgc ttgaggagat actccttgca ctggaacggg atgccggcct
     2461 gcagcttctt ccgggtaaag cgctcttcgt tggccacgaa gagcatctcc ccctcggcgg
     2521 tggtcaggca gaggctggaa tcgtggctct tgtggaagcc gatatagagg atgtccgaca
     2581 tcccgggcgt actcctgggc gttcgtgggg caagccgacc ggggccgggt cgttgcgacc
     2641 gacaccgggt cgaaaacaga agcgacgagt atacccgaac gctcctgcct gccgctaaca
     2701 aaagctctgt ccaagggtta tcggcacccg gggggcccga cctgaagcgg tttcgggggg
     2761 attggcccgg ttggggaagg cccaggggcc tgctgaaaca ccggtaggac gggggggacc
     2821 tgtcatgctt tcagtccact gtggctagcc acagttgtca gcggctatcg tctcccgatg
     2881 gccggcctct tcgatcccct cccgatccgc ggcgtca
//
LOCUS       DASVOD010000766         3659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_207756, whole genome shotgun sequence.
ACCESSION   DASVOD010000766 DASVOD010000000
VERSION     DASVOD010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207756"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..765
                     /locus_tag="VF720_16405"
     CDS             325..765
                     /locus_tag="VF720_16405"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX7880994.1"
                     /translation="MTLVRYRPFGRTMSVADEMNQMLDSFFTGSNSNPSSPNRWSPPV
                     DVRESETGFTVRVEVPGVAKDQIHLNLTNNTLTISGDKKQVVDESKGQWHHVERAWGS
                     FERSITLPVRVDGEKIVARHQDGVLEITVPKSDEAKPREIKIES"
     gene            861..2753
                     /gene="dnaK"
                     /locus_tag="VF720_16410"
     CDS             861..2753
                     /gene="dnaK"
                     /locus_tag="VF720_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010932327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX7880995.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGGEPVVIPNSEGARTTPSVVAFTK
                     SGERLVGQQAKRQAVTNPKNTVYSIKRFMGRGFDEVGGEMKEVPYEVTKGTGGQVAVK
                     VGDKEFTPPEISAMILQKMKQTAEDYLGQKVTEAVITVPAYFNDSQRQATKDAGRIAG
                     LDVKRIINEPTAAALAYGLDKKKNEIVAVYDLGGGTFDISILEISEDGVFQVKSTNGD
                     THLGGDDFDQRVMDWLADEFKKSDGIDLRNDAMALQRLKEAAEKAKIELSSSMQTDIN
                     LPFITADQNGPKHLNLSLTRAKLEQLVGDLVDRTVGPCQKAISDAKVTASQIDEVILV
                     GGSTRMPKVQEMVKKLFEKEPHKGVNPDEVVAIGAAIQGAVLAGDVKEVLLLDVTPLS
                     LGIETLGGVFTRLIDRNTTIPTKKMETFSTAADSQTAVDIQVFQGEREMAAHNKQIGH
                     FQLDGIPPAPRGVPQVEVTFDIDANGILSVSARDKATNKEQSIKIQASSGLTEDEIKN
                     MVKDAEAHSADDKKKRAEIEARNKADALAYETEKQLKESGDKLDADTKARVEAAVTRV
                     REALKTDNVDEITSSAEALTQVWHEAAQKMYAAAGAGPQGPGPDAAGGAPPKDKTVDA
                     DFEVVE"
     gene            2854..3201
                     /locus_tag="VF720_16415"
     CDS             2854..3201
                     /locus_tag="VF720_16415"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX7880996.1"
                     /translation="MLVRLMVSWVHPQVMMFLGKVKNPTTDTMERNLDAARTLIDVLA
                     ELQSKTEGRLDPDDQRLLQHTLTELRMNWLDESNKDAGGAPADGGGGGADDVEDPAAG
                     PTPSAGGTPPAGL"
     gene            3201..>3659
                     /locus_tag="VF720_16420"
     CDS             3201..>3659
                     /locus_tag="VF720_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX7880997.1"
                     /translation="MAKRDYYEVLGVPENATEEQIKKAFRTQAKKYHPDANRNDPSAE
                     AKFKEAGEAFEVLGDAAKRQQYDRMKRCGGHPGGFPSGGVPGGYPGGADGGTFRGETI
                     DLSDLFGGGGGGRGMGDIFEHLFGGRYRQAEEPEAERDIAVALDVPARVAE"
BASE COUNT          787 a         1114 c         1214 g          544 t
ORIGIN      
        1 caacgtcagc cgcagcgcgc gtcagcttgg catctctcgc tcgacaatgt ggcgcaagat
       61 gaaacagtat ggactcggat aacgctgtaa gtcgttattc ttgacgcgtc tgcttcaaaa
      121 tggcacaacg actgtctcaa aatgaagcgg taggggttgc gcacggcaga ggggtcatca
      181 tgaattattg ttctataacc gattgtcatt gaattgttta acctcccgga aacacccgaa
      241 attcttcact ggcacatccc ctgcaaagtt ggcccgacag cacggtcctt cggcttgaac
      301 caacgacaca ggaggtgttt caaaatgacg cttgtccgtt accgcccgtt cgggcgtacg
      361 atgtcggttg ccgacgagat gaaccagatg ctggactcgt tcttcaccgg ctcgaattcc
      421 aacccttcga gtccgaaccg ctggtcgccg ccggtggatg tccgggagtc ggagacgggc
      481 ttcaccgtcc gggtggaagt acccggggtg gcgaaggacc agatccattt gaacttgacc
      541 aacaataccc tgacgatcag tggcgacaag aagcaggtcg tcgacgagtc gaagggacag
      601 tggcaccacg tcgaacgggc gtggggctcg ttcgagagga gcattacgct tccggtccgc
      661 gtggatggtg agaagatcgt cgcgcggcac caggacgggg tgctcgagat cacggtaccg
      721 aagtccgacg aagccaagcc acgcgagatc aagatcgagt cgtaaatcca ggcccgatcg
      781 aaaatcaacg agaggcggtc ggctccggtc ggccgcctcc tttacataga tcgtcaatta
      841 aaaagaaacg aggggaacac atgggcaaga tcatcggcat cgacctcggc accacgaatt
      901 cgtgcgtagc cgtcatggaa ggcggcgagc ccgtcgtcat tccgaactcg gaaggcgcgc
      961 gcaccacgcc gtccgtggtg gcattcacca agagcggtga gcgcctcgtg gggcagcagg
     1021 ccaagcgtca ggccgtcacc aacccgaaga acaccgtcta ctcgatcaag cgcttcatgg
     1081 ggcgcgggtt cgacgaagtc ggcggcgaaa tgaaggaggt cccctacgag gtgaccaagg
     1141 ggaccggcgg ccaggtggcg gtgaaggtcg gcgacaagga attcacgcct cccgagatct
     1201 cggccatgat cctccagaag atgaagcaga ccgccgagga ctacctaggc cagaaggtca
     1261 ccgaggccgt catcaccgtg ccggcctact tcaacgattc gcagcgccag gccaccaagg
     1321 acgcggggcg catcgccggc ctcgacgtga agcgcatcat caacgagccc accgccgccg
     1381 cgctggccta cggcctcgac aagaagaaga acgagatcgt ggccgtgtac gacctgggcg
     1441 gcggtacttt cgatatctcg atcctcgaga tctcggaaga cggcgtcttc caggtgaaga
     1501 gcaccaatgg cgacacccac ctgggtggcg acgacttcga ccagcgggtc atggactggc
     1561 tggccgatga gttcaagaag tcggacggca tcgacctgcg caatgacgcg atggcgctcc
     1621 agcggctgaa ggaagccgcc gagaaggcca agatcgagct gtcgtcgtcg atgcagaccg
     1681 acatcaacct gccgttcatc acggccgacc agaacggtcc caagcacctc aacctttccc
     1741 tgacgcgggc caagctcgag cagctggtgg gcgacctggt cgaccgcacc gtcggtccgt
     1801 gccagaaggc catctccgat gccaaggtca cggcaagcca gatcgacgaa gtcatcctcg
     1861 tgggcggttc gacgcgcatg cccaaggtgc aggagatggt gaagaagctc ttcgagaagg
     1921 agccgcacaa gggggttaac ccggacgagg tcgtggccat cggcgccgcc atccagggcg
     1981 cggtcctggc gggcgacgtg aaggaggtcc tcctcctcga cgtgacgccg ctgtcgctcg
     2041 gcatcgagac gcttggcggc gtgttcacgc ggctcatcga ccggaacacc accatcccga
     2101 cgaagaagat ggagaccttc tccacggcgg ccgacagcca gaccgcggtc gacatccagg
     2161 tgttccaggg cgagcgcgag atggcggccc acaacaagca gatcgggcac ttccagctgg
     2221 acggcatccc gccggccccg cgtggcgtgc cgcaggtgga agtgaccttc gacatcgacg
     2281 ccaacggcat cctgtccgtt tcggcccgtg acaaggccac caacaaggag cagagcatca
     2341 agatccaggc ctcgtccggc ctcaccgagg acgagatcaa gaacatggtg aaggatgccg
     2401 aggcgcactc cgccgatgac aagaagaagc gtgccgagat cgaggcccgg aacaaggccg
     2461 atgccctggc ctacgagacc gagaagcagc tcaaggaatc gggggacaag ctcgacgccg
     2521 acacgaaggc cagggtggag gccgcggtca cccgcgtgcg cgaggccctg aagacggaca
     2581 acgttgacga gatcacctcg tcggccgaag ccctgacgca ggtctggcac gaggcggccc
     2641 agaagatgta cgcggcggcc ggcgccggtc cgcaggggcc gggtccggat gcggccggtg
     2701 gtgcgccacc gaaggacaag acggtggacg ccgacttcga ggtcgtggag taatcgttcg
     2761 accggcaccg accggccgga gcaacggcaa cattctaatg acggcgggcg gggctccatc
     2821 ggggtcccgc ccgttcgtat cgggaggaac gtgatgctcg tccgcttgat ggtgtcatgg
     2881 gtgcatcccc aggtgatgat gttcctggga aaggtcaaga acccgacgac cgacacgatg
     2941 gagcgcaacc tcgatgcggc ccggacgttg atcgacgtgc tggccgagct ccagtcgaag
     3001 acggaaggac ggctcgaccc ggatgaccag cggctgctgc agcacacgtt gacggagttg
     3061 cggatgaact ggttggatga atcgaacaag gacgcgggag gagcgcccgc tgacggtggt
     3121 gggggcggag ctgacgatgt cgaagacccc gccgcaggcc ccaccccttc agccggcggc
     3181 acgccgccgg caggactttg atggcgaagc gggattacta cgaggtgctc ggcgttccgg
     3241 aaaacgccac cgaggagcag atcaagaagg ccttccggac gcaggccaag aaataccatc
     3301 cggacgccaa ccgtaacgac ccctcggccg aagccaaatt caaggaggcc ggcgaggcgt
     3361 tcgaggtcct gggcgacgcg gccaagaggc agcagtacga ccggatgaag cgctgcgggg
     3421 ggcaccccgg cggctttcca agcggaggcg tccccggcgg ttatcccggc ggcgccgacg
     3481 gcggcacgtt ccgcggcgag acgatcgacc tctccgacct cttcggcggc ggtggtggcg
     3541 gtcgcggcat gggcgacatc ttcgagcacc tcttcggcgg ccgttaccgc caggccgagg
     3601 agccggaggc cgagcgcgac atcgcggtgg cgctggacgt gccggcgcgc gtggcggaa
//
LOCUS       DASVOD010000767         3122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_885732, whole genome shotgun sequence.
ACCESSION   DASVOD010000767 DASVOD010000000
VERSION     DASVOD010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3122
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885732"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1800
                     /locus_tag="VF720_16425"
     CDS             154..1800
                     /locus_tag="VF720_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX7880998.1"
                     /translation="MITVTNVSKQYGRQVLFVEASFQLNSGEKAGLVGPNGAGKTTLF
                     RMLTGEETPDEGDVSVPKRVSIGYFRQDIGEMSGRSVLDEAIAGSGTLGELHHELKDL
                     EAALADPDKADQMEKILDRFGHVQDEYQQGGGYDLEARAREVLHGLGFEDRQIDGDVG
                     ALSGGWKMRVGMARVLLGRPDVLLMDEPTNHLDIESILWLEQFIKDYQGIVLMTCHDK
                     DFMNRIVDRIIAIDSGEFTTYTGNYDYYVRERTIADANKEAAYARQQAMLAKERRFIE
                     RFQTHAAKAAQVQSRVKKLEKIEIVELPKSRKVVEFDFRQPARSGDDVLAMKGVSKAY
                     GPRILYDDFNFNIRRTERWCVMGRNGAGKTTLLRLAVGALLPDSGTVRTGASLTIGYF
                     AQQSLDLLDPALTVIDQIRKDFPDAGQGALRNLLGAFQFSGTDVEKPIRVLSGGEKSR
                     LVLARMLFDPPNFLILDEPTNHLDLETKEMLVEALKGFEGTMLFVSHDRTFLKGLANR
                     VLELRPGDGNGIPVEPPLAYPGTYLEYVERTGNEAAGVHR"
     gene            1935..>3122
                     /locus_tag="VF720_16430"
     CDS             1935..>3122
                     /locus_tag="VF720_16430"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX7880999.1"
                     /translation="MRFLRNRLSVFALSLLGVLPALLAPAARPGVVHAAPAAAIPSDD
                     ALGARLDAYLKPMFPADKPGAVVLVARNGKPIYRKAFGMADMATRTSLTPESVLRIGS
                     VTKQFTAAAILRLEETGKLKVTDPIEKWLPDFPLSGHTVTIENLLTHTSGIKGYTEVP
                     AWAARMNEEMTTQQVVDFFKNEPFLFEPGTNWAYSNSGYFLLGMIIEKASGMPYKDYL
                     QQEIFGPLRMTHSGYGDRNPTFPGEARGYAMDGDKVVPAEPLSMSSPYAAGALVSSVD
                     DLLEWDNAIATDRLLHPNSSKAMWTPFKQVSGKTSGYGYGWLMGEYEGHPIQEHNGGI
                     NGFTCHAMRLPEDKLYVVVLTNSDQPPADPGYIALTLASEALGRPMPQPGDFILAPEK
                     LDEF"
BASE COUNT          563 a         1091 c          987 g          481 t
ORIGIN      
        1 gttcgggcgc gtggagcggg catctccgcc acgacccgca tcgtcttcgt ggattgacgg
       61 cgtccgcgcc gggccaacgg cggccggacc gggcacgttc gggcggcgtt gccacccagg
      121 cttaccacgc cgtctgctaa actccccgcc ctcatgatca ccgtaaccaa cgtcagcaag
      181 cagtacggcc gccaggtcct cttcgtcgag gcctcgttcc agctcaattc gggcgaaaag
      241 gccggcctgg tgggcccgaa cggggccggc aagaccacgc tcttccggat gctcaccggc
      301 gaggagacgc ccgacgaggg ggatgtctcc gtcccgaagc gggtcagcat cggctacttc
      361 cgccaggaca tcggggagat gtcgggccgg agcgtcctgg atgaggccat cgccggcagc
      421 ggcacgctcg gcgaattgca ccacgagctc aaggacctcg aggccgccct ggccgatccg
      481 gacaaggccg accagatgga gaagatcctc gaccgcttcg gccacgtgca ggacgaatac
      541 cagcaggggg gcggctacga cctggaggcc cgcgcccgcg aggtgctcca cggcctcggc
      601 ttcgaggacc ggcagatcga cggcgacgtc ggcgccctct ccggcggctg gaagatgcgc
      661 gtggggatgg cgcgcgtgct gcttggccgc ccggatgtcc tcctcatgga cgagcccacc
      721 aaccacctcg acatcgaatc gatcctttgg ctcgagcagt tcatcaagga ctaccagggg
      781 atcgtcctca tgacctgcca cgacaaggac ttcatgaacc ggatcgtcga ccggatcatc
      841 gccatcgact cgggcgagtt cacgacgtac accggcaact acgactacta cgtgcgcgag
      901 cgcacgatcg ccgacgccaa caaggaggcc gcgtacgcgc gccagcaggc catgctggcc
      961 aaggagcgcc gcttcatcga gcgcttccag acgcatgccg ccaaggccgc ccaggtgcag
     1021 agccgcgtca agaagctcga gaagatcgag atcgtcgagc tgccgaaatc gcgcaaggtg
     1081 gtggaattcg acttccgcca gccggcccgc tcgggggatg acgtcctggc catgaagggc
     1141 gtgtcgaaag cctacggccc gcgcatcctg tatgacgact tcaacttcaa catccgccgc
     1201 accgagcgct ggtgcgtcat gggtcggaac ggcgcgggca agaccacgct cctccgcctg
     1261 gccgtcggcg cgctcctgcc cgactcggga acggtgcgca ccggcgcctc gctcacgatc
     1321 ggctacttcg cccagcagtc gctcgacctg ctcgatccgg ccctcaccgt catcgaccag
     1381 atccgcaagg atttcccgga cgccgggcag ggggcgctgc gcaacctcct cggcgcgttc
     1441 cagttctcgg gcaccgatgt cgagaagccg atccgcgtcc tctccggcgg cgagaaatcg
     1501 aggctggtcc tggcccggat gctgttcgac ccacccaact tcctgatcct ggacgagccc
     1561 accaatcacc tcgatctcga gaccaaggag atgctggtcg aggcgctcaa ggggttcgag
     1621 gggacgatgc tcttcgtcag ccacgaccgc accttcctca aggggctggc caaccgggtg
     1681 ctcgagctgc gccccggcga cggcaacggc atcccggtcg agccgccgct ggcgtacccc
     1741 ggcacgtacc tggaatacgt ggaacggacc gggaatgagg cggctggggt gcatcggtag
     1801 ggccgacccc ccaccgagcg cgatgaatcg accgcgaaaa caggttggct aaccgacgca
     1861 acctgaagtc gacatagaac gtagcccgac tctcgaacca cggcctcacg ttgacgcatt
     1921 ccaaggggga ccctgtgcgc ttccttcgta accgactgtc cgtctttgcc ttgtcgctcc
     1981 tcggcgtcct gccggcgctc ctggccccgg cagcccgtcc gggcgtggtc catgccgcac
     2041 cggccgccgc catcccgtcc gacgatgccc ttggcgcccg cctcgacgcc tacctgaaac
     2101 cgatgttccc cgccgacaag cctggcgcgg tggtcctggt ggcgcggaac gggaagccga
     2161 tctatcggaa agcgttcggc atggccgaca tggccacgcg cacgtccttg acacccgagt
     2221 cggtccttcg catcggctcg gtcaccaagc agttcaccgc ggccgccatc ctccgcctcg
     2281 aggagacggg gaagctcaag gtcaccgacc cgatcgagaa gtggcttccc gattttccgc
     2341 tctcggggca tacggtcacg atcgagaacc tcctcacgca cacgtcgggc atcaagggct
     2401 ataccgaggt tccggcgtgg gcggcgcgca tgaacgaaga gatgacgacg cagcaggtcg
     2461 tcgacttctt caagaacgag ccgttcctct tcgagccggg cacgaactgg gcctactcga
     2521 attcgggcta tttcctcctc ggcatgatca tcgagaaggc cagcggcatg ccgtacaagg
     2581 actacctcca gcaggagatc ttcggcccgc tcaggatgac ccactcgggt tacggcgacc
     2641 ggaaccccac gtttcccggc gaggcgcgcg gctacgcgat ggacggcgac aaggtcgtgc
     2701 cggccgagcc gctctcgatg tcgagcccgt acgcggccgg cgccctcgtc tcgtcggtgg
     2761 acgacctcct cgagtgggac aacgcgatcg ccaccgaccg gctcctccat ccgaactcgt
     2821 cgaaggcgat gtggaccccg ttcaagcagg tcagcggcaa gacgagcggc tacggctacg
     2881 gctggctgat gggggagtac gaaggtcacc ctatccagga gcacaacggc ggcatcaacg
     2941 gcttcacctg ccacgccatg cgtcttccgg aggacaagct ctacgtggtc gtcctcacga
     3001 acagcgacca gccgccggcc gacccggggt acatcgcact cacactggcg tcggaggcgc
     3061 tcggccggcc gatgccgcag ccgggcgatt tcatcctggc gccggagaag ctcgatgagt
     3121 tc
//
LOCUS       DASVOD010000768         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_312670, whole genome shotgun sequence.
ACCESSION   DASVOD010000768 DASVOD010000000
VERSION     DASVOD010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312670"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1899)
                     /locus_tag="VF720_16435"
     CDS             complement(172..1899)
                     /locus_tag="VF720_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881000.1"
                     /translation="MRRFTPVRRLGLLLFSTTLLYAPCVSAAPLLRDAPVTWKEDDRR
                     PIPKPEVRDPNLLWDGIDQTFFGPISRVTDPGRLLRRASVPFGSDHVRAAVNVNALDE
                     VPNSSWFTNRIGLAPMSPEEVALGPGLAYGAESAAGPDTTGDWTVVRAKTEGVTPGFN
                     IRDSRGHVYVIKFDDPRYPHMSSAAGVITGRLLHAAGYNVTADYIVYFERAHLKLGEK
                     VNFTPRDGAKRPMTEADIDAILALVPPEKDGRWRALASRYLPGEPAGPFNWESRRRDD
                     PNDRIDHEDRRELRGFRMFAAWLAHFDTKQGNTLDMWQEQEGGKSLRHYFIDFASTLG
                     AGATGPFRTYSYEYSFDFPAIGGRLLSLGFHDDKWRQLRIPGGLTEVGYFQSDTFDPM
                     EFKPLTPNASYANMTERDAYWAAKIISAFTNADLESAVATGQYENPEAARWIAQMLAE
                     RRDKIARKMFDEVPPLDFFRMSGTASISFHDLGTERGIYPGAVARYRARFQVVDAEDD
                     GEGWSDWSESPDPTFEIDAPGLNGLSRDDQGNNFLAFEFQVNRGDGWSRSVRAWGSTT
                     GNGIIAVEH"
     gene            complement(1899..>2725)
                     /locus_tag="VF720_16440"
     CDS             complement(1899..>2725)
                     /locus_tag="VF720_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881001.1"
                     /translation="PEDEAFSGLDTTWGGLGVNRRLGGTWVLQASALANSAGTRPPAE
                     EGDQSVGEVFPDDIPYGYAERSDGATFGLDLAAGEPRTALRPDDEALLRVHVSYFDDW
                     NNASPSYWAGRVSARRFFPLWFTGRTLGLRGMIAWSDPVGPGEIPFQRMNSNHGLDRF
                     RGYQDFRFTDRGMTLFTAEYRWPIWVTREALSPGLDFILLADAGQVFRQVDQIAIDRL
                     AESYGFAFEYAGSRGLLARVEFAWSNEDRVIRFGLVNLLETGFGQLFQGRIMDREP"
BASE COUNT          448 a          847 c          985 g          445 t
ORIGIN      
        1 agggaaacgg cagcaaacag ggtcaggcca acgatcaggc ccggcaggag tcggcggata
       61 tggctcatgg gggtccccaa ggggtggatc gaggaggcgg tcggcggatc gaaccaaccc
      121 cccgggggcc ggttccccaa catgatagcg gatcgggatg gggcggactt gtcaatgttc
      181 gacggcaatg atcccgttgc cggtggtgct cccccaggcg cggaccgagc gactccagcc
      241 gtcgcctcgg ttcacctgga attcgaaagc gaggaaattg tttccctggt cgtctctcga
      301 caggccgttc agcccgggcg catcgatttc gaacgtcgga tcgggtgatt ccgaccagtc
      361 ggaccaaccc tctccgtcat cttccgcgtc cacgacctgg aatcgggcgc ggtagcgcgc
      421 aaccgctccg gggtagatgc cgcgctcggt gccgaggtca tgaaaggaga tcgaggccgt
      481 cccgctcatc cggaagaaat cgagcggcgg cacctcgtcg aacatcttcc gcgcgatctt
      541 gtcccgccgc tccgccagca tctgcgcaat ccaccgggcc gcttcgggat tttcgtactg
      601 tccggtggcc acggccgact cgaggtcggc gttcgtgaag gcggagatga tcttggcggc
      661 ccagtaggcg tcgcgctcgg tcatgttggc gtagctggcg ttgggtgtga ggggcttgaa
      721 ctccatgggg tcgaaggtgt cgctctggaa gtagccgact tcggtcaggc cgccggggat
      781 ccggagctgg cgccatttgt cgtcgtggaa cccgagcgag aggaggcggc cgccgatcgc
      841 ggggaagtcg aacgagtatt cgtagctgta ggtgcggaag gggccggtgg cgccggcgcc
      901 gagcgtgctg gcgaagtcga tgaaataatg gcggagcgac ttgccccctt cctgctcctg
      961 ccacatgtcg agcgtgttcc cctgcttggt atcgaaatgg gccagccagg ccgcgaacat
     1021 gcggaagccc cggagctcgc ggcggtcctc gtgatcgatc cggtcgttgg gatcgtccct
     1081 gcggcggctt tcccagttga aggggccggc cggctcgccc gggagatagc ggctggcaag
     1141 ggcgcgccag cggccgtcct tttcgggcgg cacgagcgcc aggatggcgt cgatgtcggc
     1201 ttcggtcatc gggcgcttcg cgccatccct gggcgtgaag ttgacctttt ccccgagctt
     1261 caggtgcgcg cgctcgaagt aaacaatgta gtcggccgtc acgttgtaac cggcggcgtg
     1321 gagcaggcgc ccggtgatga cgccggcggc gctcgacatg tgcgggtagc gcgggtcgtc
     1381 gaacttgatc acgtacacgt gcccgcggga atcccggatg ttgaatccgg gcgtcacgcc
     1441 ttcggtcttg gcgcgcacca ccgtccagtc gcccgtggta tccggaccgg cagccgactc
     1501 ggcgccgtag gccaggcccg ggccgagcgc cacttcctcg ggggacatcg gagcaaggcc
     1561 gatccgattc gtgaaccagg aggagttcgg cacctcgtcc agcgcgttga cgttgaccgc
     1621 ggcgcgcacg tgatcgctgc cgaacggcac actggcgcgc cggaggaggc gccccgggtc
     1681 ggtcacgcgg ctgatcggac cgaagaacgt ctggtcgatg ccgtcccaca ggagattggg
     1741 atcgcgcact tcgggcttcg ggatcgggcg gcggtcgtct tccttccagg tgacgggcgc
     1801 atcgcgaagg agcggcgcgg ccgacacgca tggcgcgtaa aggagtgtgg tcgagaagag
     1861 gaggagtccg aggcgtcgga cgggggtgaa gcggcgcatc acggctcccg gtccatgatc
     1921 cgcccctgga agagctggcc gaatccggtt tcgagcaggt tgaccaggcc gaagcggatg
     1981 acgcggtctt cgttgctcca ggcgaactcc acgcgggcga ggaggccgcg cgagccggcg
     2041 tattcgaaag cgaagccgta cgactcggcc agccggtcga tcgcgatctg gtcgacctgg
     2101 cggaagacct ggccggcatc ggcgagcagg atgaaatcga gaccgggcga aagcgcctcc
     2161 cgcgtcaccc agatcggcca gcgatattcc gcggtgaaga gcgtcatgcc gcggtccgtg
     2221 aatcggaagt cctggtagcc gcggaagcga tcgaggccgt ggttggagtt catccgctgg
     2281 aacgggattt cgccagggcc taccgggtcg gaccaggcga tcatgcccct gaggccaagc
     2341 gtgcggccgg tgaaccacag cgggaagaac cggcgggcgc tgaccctccc ggcccagtag
     2401 gacggcgagg cgttgttcca gtcgtcgaaa tacgaaacgt gcacgcgcag cagcgcctca
     2461 tcgtcggggc gcagggcggt gcgcggttcg ccggccgcga gatcgagccc gaacgtggcg
     2521 ccatcactcc gctcggcgta tccgtacggg atgtcatcgg ggaagacttc gccgacactc
     2581 tgatcacctt cctcggccgg cggccgcgtg ccggcagagt tggcgagggc gctggcctgg
     2641 agcacccagg tgccgcccag ccggcggttg acgccgaggc ccccccaggt cgtgtccagc
     2701 ccggagaacg cctcgtcctc gggat
//
LOCUS       DASVOD010000769         5533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_208115, whole genome shotgun sequence.
ACCESSION   DASVOD010000769 DASVOD010000000
VERSION     DASVOD010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208115"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VF720_16445"
     CDS             <1..509
                     /locus_tag="VF720_16445"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX7881002.1"
                     /translation="HDQHALKAFEVHALDYLLKPYTAARFQAALARVRAELADADDFP
                     ARARLGRLLDARDPAPGYLHRFTVRDRDRIVFVRAEEVEAIAAAGNYLELVTTAGTHL
                     VRMTLSEAESRLDPAKFARIHRSTIVNKSRIREIKPDSHGDADVIMVSGASHRLSRAY
                     GKGTLLDP"
     gene            complement(516..2291)
                     /locus_tag="VF720_16450"
     CDS             complement(516..2291)
                     /locus_tag="VF720_16450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX7881003.1"
                     /translation="MRNDIGRRMAAILIPLLTLPSPLFAQAFTTFTRITDPANPIVTE
                     TGHQSGGGCWVDLVGDGYLDLFVANGNLSNELNSLYRNNRAGGFVRVTTGSVRNDGGS
                     SIGGTVGDYDNDGKIDLFVTNRNFFGNFLYRGLGDTNFVKVTGAHPVTDLGNSNSASW
                     VDVDRDGNLDLYVVNFQGADYFYRNGGAPAYAFTRVDTTALTAGGEFSIPGAWGDYNN
                     DGRPDLFIGNAGTQSDYLYQNRGNLWFTRTSIADGGATLGASWGDFDNDGDLDLVVPH
                     FQGQKIKFYRNSGAPLYQLASIDTGAVSASFGNWIGSGWGDCDNDGDLDLVVTYDGAP
                     AGLFRNDGPPNHGFTRIVTGPVGTDTGYNFGAVWGDYDRDGQLDLFIANRLGEQNRLY
                     HNDGNANHWLTIRAVGTVSNRSAIGARIRLHSKVAGVPRSQMREVTGQTGYNSQNLDQ
                     HFGLADAAVADSIVIDWPSGAREVWRGVLADRWMTLIEGGSTVAVPERPQPVSGGVLF
                     APPRPNPARGAVRLSFRLAVPGDVTLALFDVRGRRMRSLLAERRPAGSNEISWRPDES
                     IPDGVYFVRLEAGVESRTERLVIAR"
     gene            complement(2376..3623)
                     /gene="pepT"
                     /locus_tag="VF720_16455"
     CDS             complement(2376..3623)
                     /gene="pepT"
                     /locus_tag="VF720_16455"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008694872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEX7881004.1"
                     /translation="MLTINQERLLERFLRYVAVDTTAGEAPIGDGPSSPGQLVLGKLL
                     RDELLALGLTDAEQDRHGIVTATIPATVDGAVPVLCFNSHVDTSPETTGANVKPNVIR
                     SYAGGDIVLSGDTSKVIRTTSNPELNDLLGKTLITTDGTTLLGGDDKAGVAIIMEAAA
                     ALLENPSVPHGKIRVMFTCDEEIGHGVDHVDLEKLGADAAYTVDGMAAGRLDVETFSA
                     DGAVVTVKGVNIHPMIAKDRMINAIRAAGEFCARLPKETLAPERTDGRDGFIHPVRME
                     GGVAEVTIHLILRSFDTPALDDYRKKLESVAKEAMAAVPGSTITVATHAQYRNLADGL
                     AKAPRVVKNVHEAYRRLNRPSTETIVRGGTDGSRLTELGLPTPNLSSGQHNPHSPLEW
                     ACLDEMVETCQVIGELVQVWAER"
     gene            complement(3655..4131)
                     /locus_tag="VF720_16460"
     CDS             complement(3655..4131)
                     /locus_tag="VF720_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881005.1"
                     /translation="MAAKLLDTLLQAEVEFVVVGGLAAVLHGSTLLTRDLDICVPIGA
                     RSFLKLQSALRPLNPRVRAGSGVIPLELDTESAARLTNLYLRTDDGRLDCLGSVAGLG
                     DYEKVLAASEKLTVHGYPLFVLGLDGLIVAKEAMGRPRDLETVTQLKAIRERLNRH"
     gene            complement(4112..4318)
                     /locus_tag="VF720_16465"
     CDS             complement(4112..4318)
                     /locus_tag="VF720_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881006.1"
                     /translation="MNRVQGKDPAVNSAWQAAALAGHDMDMLEESIELSPEERLRLHE
                     LGMERVRLLEAAMTQARADGRETA"
     gene            complement(4361..>5533)
                     /gene="creD"
                     /locus_tag="VF720_16470"
     CDS             complement(4361..>5533)
                     /gene="creD"
                     /locus_tag="VF720_16470"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEX7881007.1"
                     /translation="NSQSITGPALVIPYTHRWTEVTAAGKEVSRTETRHAVFLPDRLT
                     ARGNLDGDVRRRGIFAVPVYTLGMALEGEFARPDVASLDINPEDVAWERAQLAVGVSD
                     ARAIQEASSVTWDGRAVPFLPGTGAFRDTETGIHAEVGLPAGSGPIAFSFPLTMNGSL
                     GLYLAPFARTTRLEIRSDYPHPSFQGNWLPDERAVTANGFQATWSIPFLGRNYPQAWR
                     AEIGMSKEIAASRFGVELVNPVDQYRMAARSVKYAGLFILLTFATVWLIELLSGVRVH
                     PIQYLLLGAALCLFYLLELSLSEHLGFPTAYGVASLAVIVMVASYGLVMLRRAGRALT
                     IGAGVTLLYGYLYILLVNEDYALLIGSVGLFLILAAIMFVTRRVAWYGSGDRMVAG"
BASE COUNT          914 a         1817 c         1866 g          936 t
ORIGIN      
        1 cccacgatca gcacgccctg aaggcgttcg aggtgcacgc gctcgactac ctgctgaagc
       61 cgtacaccgc ggcgcgcttc caggccgccc tggcccgcgt ccgcgccgag ctggccgacg
      121 ccgacgactt tcccgcccgg gcccggctgg gccgcctcct cgacgcgcgg gacccggcgc
      181 ccgggtacct ccatcgcttc accgtgcgcg accgcgaccg gattgtcttc gtgcgggcgg
      241 aggaggtgga ggcgatcgcg gcggcgggga actacctcga gctggtgacc acggccggca
      301 cccacctcgt gcggatgacg ttgtcggaag cggaatcccg gctggatccg gcgaagttcg
      361 cacggatcca ccgctcgacg atcgtgaaca agtcacgtat ccgcgagata aaaccggatt
      421 cgcacggcga cgccgacgtc atcatggtca gtggcgcctc gcaccgtctc agccgggcct
      481 acggcaaggg tacgctcctg gatccctgac gcacgctacc gcgcgatgac caggcgctcg
      541 gtgcgcgact cgacgccggc ctcgagtcgt acgaagtaga cgccgtcggg aatcgactcg
      601 tccggccgcc aggaaatctc gttggatccg gcgggacgcc gttcggccag cagcgaccgc
      661 attcgccgcc ccctcacgtc gaacagcgcg agcgtcacgt cgcccggcac cgccaggcgg
      721 aatgaaagcc tcacggcgcc ccgggccgga ttgggtcggg gtggcgcgaa cagcacgccg
      781 ccggaaaccg gctgcggccg ttcgggcacc gccaccgtcg accccccttc gatcaacgtc
      841 atccaccggt cggccagcac accccgccaa acctcgcgcg cgcccgacgg ccagtcgatc
      901 acgatcgagt cggccacggc cgcgtccgcg aggccgaagt gctggtcgag gttctgcgag
      961 ttgtagcccg tctggccggt cacctcgcgc atctggctgc gcggcacgcc ggccaccttc
     1021 gagtggagcc ggatccgcgc cccgatggcc gagcggttgg aaaccgtgcc caccgcgcgg
     1081 atcgtcagcc agtgattggc attcccgtcg ttgtggtaca gccggttctg ttccccgagc
     1141 cggttcgcga tgaaaaggtc gagctgcccg tcccggtcgt aatcgcccca cacggcaccg
     1201 aagttgtagc cggtgtcggt gcccactggt ccggtcacga tccgggtgaa gccgtggttc
     1261 ggcggcccgt cgttgcggaa gaggccggcc ggcgcgccgt cgtatgtcac gaccaggtcg
     1321 aggtcgccgt cgttgtcgca atccccccag ccgctcccga tccagttgcc gaacgaagcg
     1381 cttaccgcgc ccgtatcgat cgaagccagc tggtagagcg gggcgccgct gttccggtaa
     1441 aacttgatct tctgcccctg gaagtggggg accacgaggt cgaggtcccc atcgttgtcg
     1501 aaatcacccc agctcgctcc gagcgtggcg ccgccgtcgg cgatgctggt gcgggtgaac
     1561 cagagattgc cgcggttctg gtaaaggtag tcgctctgcg tcccggcatt gccgatgaaa
     1621 aggtccggcc gcccgtcgtt gttgtaatca ccccaggcgc cggggatcga gaactcgcct
     1681 ccggccgtca acgccgtcgt gtccacgcgc gtgaaggcgt aggccggagc gccgccgttc
     1741 cgatagaagt aatcggctcc ctggaagttg acgacgtaga ggtcgaggtt gccgtcccgg
     1801 tcaacgtcca cccacgaggc gctgttggaa ttgccgaggt cggtcaccgg gtgcgcgccg
     1861 gtcaccttca cgaagttcgt gtcgccgagg ccgcgataga ggaagttgcc gaaaaaattc
     1921 cggttcgtga cgaagaggtc gattttcccg tcattgtcgt agtcgccgac cgtcccgccg
     1981 atcgacgacc caccatcgtt ccgcacgctc cccgtcgtca cgcgcacgaa tcccccggcc
     2041 cggttgttcc ggtacagcga gttcaactcg ttcgacaggt tgccgttggc cacgaagagg
     2101 tcgaggtagc catcaccgac gagatccacc cagcagccgc cgccgctctg gtgtccggtt
     2161 tccgtcacga tcgggttggc cgggtcggtg atgcgggtga acgtcgtgaa cgcctgggca
     2221 aacaggggcg acgggagagt caggaggggg atgaggatgg ccgccatccg gcggccgatg
     2281 tcgttgcgca tgctccagtc ttgacgtgaa accggccggc cgcatgggtc ccgggaccag
     2341 tcggtggggg cgggggatga acggggggaa aggctttacc tctccgccca cacctgcacg
     2401 agctccccga tcacctggca ggtttccacc atttcgtcga ggcaggccca ttccagcggc
     2461 gagtgagggt tgtgctgccc gctggagagg ttgggcgtgg ggaggccgag ctccgtgagg
     2521 cgcgagccgt cggtgccgcc ccgcacgatg gtctcggtcg acggtcgatt aagccggcga
     2581 taggcctcgt gcacgttctt caccacccgt ggcgctttgg ccaggccatc cgccaggttc
     2641 cggtactggg cgtgtgtggc cacggtgatc gtggagccgg gaacggccgc catggcctcc
     2701 ttcgccaccg attccagttt cttccggtag tcatcaagcg caggcgtgtc gaaggaacgc
     2761 aggatcaggt ggatcgtgac ctcggccacg cccccttcca tccgcaccgg gtggatgaag
     2821 ccgtcccgac catcagtgcg ctcgggcgcc agcgtctcct ttggcagccg cgcgcagaat
     2881 tcgcccgccg cgcggatcgc gttgatcatc cggtccttgg cgatcatggg gtggatgttg
     2941 acccccttga ccgtcacgac cgcgccgtcg gccgagaacg tctcgacgtc gagccggccg
     3001 gcggccatgc cgtccacggt gtaggcggcg tccgcgccga gcttttcaag gtccacgtgg
     3061 tccacgccgt gcccgatctc ctcgtcgcag gtgaacatca cccggatttt tccgtgcggc
     3121 acggaagggt tctcgagcag cgcggcggca gcctccatga tgatggccac gcccgccttg
     3181 tcgtcgccgc cgagcagcgt ggtgccgtcg gtcgtgatga gcgtcttgcc gagaaggtcg
     3241 ttgagctccg gattgctggt ggtccggatc accttcgagg tgtctccgga caggacgatg
     3301 tcgccccccg catacgagcg gatgacattc ggcttcacgt tcgcgcccgt tgtttccggc
     3361 gaggtgtcga cgtgcgaatt gaagcagagc accggcaccg ccccatccac ggtggccgga
     3421 atggtggccg tcacgatgcc gtgccgatcc tgctccgcgt ccgtgaggcc cagcgccaga
     3481 agttcatctc gcagcagttt cccgagaacg agctggccgg gggatgaagg cccatccccg
     3541 attggcgcct caccggcagt ggtgtcgacg gccacgtaac ggagaaaacg ttcgagcagg
     3601 cgttcctggt tgatggtcag catggggttc ctcgggttct gctctgcagg tgagtcagtg
     3661 gcgattcagt cgttcgcgta tcgccttgag ctgggtaacg gtttccaggt cgcggggacg
     3721 tcccatcgct tccttcgcca cgatcaatcc gtcgagtcca agcacgaaga gtggataacc
     3781 atggacggtc agtttctcgc ttgcggcgag caccttctcg taatccccga ggccggctac
     3841 cgatccaagg cagtcaaggc gaccatcgtc cgttctcagg tagaggttcg tcagtcgcgc
     3901 cgcgctttcg gtgtccagct cgagcgggat gacaccggag ccggccctga ctctcgggtt
     3961 gagtggccgc aacgcggatt gcagtttcag gaaggaccga gcgccgatgg ggacgcagat
     4021 gtccagatca cgtgtcagca gtgtcgaacc gtggagcacc gccgccagcc ctccgacgac
     4081 gacaaactcg acttccgctt gcagaagcgt gtcaagcagt ttcgcggcca tccgctcttg
     4141 cctgggtcat tgccgcttcg agcaacctca cgcgctccat cccaagttca tggagcctga
     4201 gcctctcttc cggcgacaat tcgatggact cttccagcat gtccatgtca tgacccgcaa
     4261 gcgctgcggc ttgccaggcg gaatttacgg caggatcctt gccttgaaca cggttcatgc
     4321 cggatagcct aagttagctg actgtatccc gcaacgcgtt tcagcctgcc accatccggt
     4381 ccccactccc gtaccacgcc acccggcgcg tcacgaacat gatggccgcc aggatgagga
     4441 agaggccgac cgagccgatc aacagggcgt agtcctcgtt cacgaggagg atatagaggt
     4501 agccgtacag gagggtgacg ccggcgccga tggtgagggc acggccggcg cggcggagca
     4561 tcacgagccc gtaggaggcc accatcacga tgacggccag gctggccacg ccgtaggcgg
     4621 tcgggaagcc caggtgctcg gaaagggaga gctcgaggag gtagaagagg cagagggcgg
     4681 cgccgagcag gaggtactgg atggggtgca cgcgcacgcc cgacaggagc tcgatgagcc
     4741 agacggtggc gaacgtgagg agaatgaaga ggccggcgta cttcacgctg cgggccgcca
     4801 tccggtactg gtcgaccggg ttgacgagct ccacgccgaa gcgcgaggcg gcgatctcct
     4861 tcgacatgcc gatctcggcg cgccaggcct gcgggtagtt gcgcccgagg aagggaattg
     4921 accaggtggc ctggaagccg ttggcggtaa cggcgcgctc gtcgggaagc cagttaccct
     4981 ggaagctggg gtggggataa tccgagcgga tttccagcct ggtcgtacgg gcgaacggag
     5041 ccaggtagag gccgaggctg ccgttcatcg tgagcgggaa ggagaacgcg atgggcccgg
     5101 agccggcggg gagccccacc tcggcatgga tgccggtttc ggtgtcacgg aaggcgccgg
     5161 ttccggggag gaacgggacg gcccgcccat cccacgtgac cgacgacgcc tcctggatgg
     5221 cgcgggcgtc ggacacgccc acggcaagct gcgcccgctc ccatgccaca tcctccggat
     5281 tgatgtcgag gctggccacg tccgggcggg caaactctcc ctccagtgcc atgccgagcg
     5341 tgtacaccgg cacggcgaaa atcccgcgcc ggcgcacgtc gccgtcgagg ttccccctgg
     5401 cggtcagccg gtcggggagg aacacggcat ggcgcgtctc cgtgcgcgac acttccttgc
     5461 ccgcggccgt cacctctgtc cagcggtggg tgtaggggat gaccagcgcc gggccggtga
     5521 tgctctggga att
//
LOCUS       DASVOD010000770         2195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_208211, whole genome shotgun sequence.
ACCESSION   DASVOD010000770 DASVOD010000000
VERSION     DASVOD010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208211"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VF720_16475"
     CDS             <1..680
                     /locus_tag="VF720_16475"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX7881008.1"
                     /translation="DPYALRRAANGIMRILMEKQWHLDLDATVAAALSTLPPTAFAKG
                     AAPDAVKIRINDFLRDRLDYFLREAGLPYDVVAATLAPVTTMSKGPVDARARANALAA
                     IRGEADLEKLVVGYKRAANILKGVDENPPADASSIEAGEPAEKALWNAWREASDALRG
                     AIDRVDYPAAVRTYLSLRAPIDDFFNGVMVNSENAAEKRRRLGLLGQVRELFDRLYDL
                     SKIVVEG"
     gene            complement(695..2167)
                     /locus_tag="VF720_16480"
     CDS             complement(695..2167)
                     /locus_tag="VF720_16480"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX7881009.1"
                     /translation="MRSIFRRRMATAFLFALPFAPLVLGGCATNPATGKKEISFVSRS
                     QEKEIGRGEDQHILAEYGTYGDTLLTAYLTDVGNRLAAQSELPSLEWHFRLLDSDVVN
                     AFALPGGYIYITRGIMAALNSEAQLAGVVGHEIGHVTARHTAQRITNQQLAGVGLMAG
                     MILSPEVARYGQALQTGAGLLLLKFGREDENQADGLGIRYMVKAGWDPREVPATYDML
                     GRLSSASGDAIPSFLSTHPDPGDRKARTSTLAIDAVTSSGKSTWTVHADEYKKRVDNM
                     VYGIDPEAGYLEDNVFYHPGLNIEITWPNGWKVTNSAANVSSTDGTAAIQLTIETTKD
                     GPSDPVAYVDYLNQKGEISAAQGQGRTINGQKAWVGRVTSSRGGQGEGDFAVAFIAKG
                     EKEFYQFVGIPGATDANGRFQNVVTSLGPIKDAGKRNARADRVDLVTAPGGKSYDQIF
                     AGEKDLALPFDKLAWLNNAFGKDVPAKGTLVKVVRRTTPA"
BASE COUNT          337 a          702 c          773 g          383 t
ORIGIN      
        1 aggacccgta cgcgctccgt cgcgcggcca acggcatcat gcggatcctg atggaaaaac
       61 agtggcacct cgacctcgac gccaccgtgg cggcggctct ctccacgctg ccgccgacgg
      121 ccttcgcgaa gggcgcggcg cccgacgccg tgaagatccg catcaacgac ttcctgcgcg
      181 accggctcga ctacttcctg cgtgaggcgg gcctgccgta cgacgtggtg gcggcaaccc
      241 tggcccccgt caccacgatg tcgaagggcc cggtggacgc gcgcgcacgc gcgaatgccc
      301 tggcggcgat ccggggcgag gccgacctcg agaagctggt cgtcgggtac aagcgggccg
      361 ccaacatcct gaagggagtc gacgagaacc cgccggccga tgcgtcatcg atcgaggccg
      421 gcgagccggc ggagaaggcg ctctggaacg cctggcgcga ggcgagcgac gcactccgag
      481 gcgcgatcga ccgggtcgac tacccggcgg cggtgcggac gtacctgtcg ctccgcgcgc
      541 cgatcgacga cttcttcaac ggcgtgatgg tcaactcgga aaacgccgcc gaaaagcgcc
      601 gtcgcctcgg cctcctcggc caggtgcgcg agctcttcga ccggctgtac gacctctcga
      661 agatcgtcgt cgaggggtag gccggcgccg actgtcaggc cggcgtcgtc cgccgcacca
      721 ccttcaccag cgttcccttg gccggcacgt ccttcccgaa ggcgttgttc agccacgcca
      781 gcttgtcgaa cggcagcgcg aggtccttct cgccggcaaa gatctggtcg tagctcttgc
      841 cacccggggc cgtgacgagg tcgacgcgat cggcgcgggc gttgcgtttg ccggcatcct
      901 tgatcgggcc gagtgacgtg accacgttct ggaagcgtcc gttggcatcg gtagcgcccg
      961 ggatgccgac gaactggtag aactccttct ctcccttggc gatgaaggcc accgcgaaat
     1021 ccccctcgcc ctggcctccc ctggaggagg tgacgcgccc tacccaggcc ttctggccgt
     1081 tgatggtgcg cccctgtccc tgcgcggcgg agatttcccc cttctgattc aggtagtcca
     1141 cgtacgccac cggatcggaa ggaccatcct tggtcgtttc gatcgtgagc tggatggcgg
     1201 cggtgccgtc ggtggaggag acgttggcgg cgctgttggt caccttccag ccgttgggcc
     1261 aggtgatctc gatgttgagg ccggggtggt agaagacgtt gtcctcgagg tatccggctt
     1321 cggggtcgat gccgtacacc atgttgtcca cccgcttctt gtactcgtcg gcatgcacgg
     1381 tccaggtcga cttgccgctg gaggtcaccg cgtcgatggc cagcgtcgag gtgcgggcct
     1441 ttcgatcgcc ggggtcgggg tgggtggaga ggaagctggg aatggcgtcg cccgaggcgg
     1501 aggagaggcg gccgagcatg tcgtacgtgg ccggcacttc gcgcgggtcc cagccggcct
     1561 tcaccatgta gcggatgccg aggccgtcgg cctggttttc atcctcccgg ccgaacttga
     1621 ggaggaggag gcccgcgccc gtctggagcg cctggccgta acgggccacc tcgggggaga
     1681 ggatcatgcc ggccatgagt cccacgccgg cgagttgctg gttggtgatc cgctgggcgg
     1741 tgtgccgcgc ggtgacatgc ccgatttcgt gcccgacgac gccggccagc tgggcctcgc
     1801 tgttcaaggc cgccatgatc ccgcgcgtga tgtagatgta gccgccggga agggcgaagg
     1861 cgttgaccac gtcggagtcg agcaggcgga agtgccattc gaggctcggg agctcggact
     1921 gggcggccag ccggttcccc acgtcggtca gataggccgt gagaagcgtg tcaccgtagg
     1981 tgccatattc ggccaggatg tgctggtctt ctcctcggcc gatctctttc tcctggctcc
     2041 gggagacgaa ggagatctcc ttcttgccgg tggcggggtt ggtggcacag ccacctagaa
     2101 ccaggggtgc gaaggggaga gcaaaaagga aagccgtcgc catccggcga cggaagattg
     2161 agcgcatgga cgatctcctt atgggcgttt gactg
//
LOCUS       DASVOD010000771         2065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_573242, whole genome shotgun sequence.
ACCESSION   DASVOD010000771 DASVOD010000000
VERSION     DASVOD010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573242"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF720_16485"
     CDS             <1..268
                     /locus_tag="VF720_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017157814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX7881010.1"
                     /translation="HVTLPGLGPVLLFVAVTTLIGFFQLFSEPYVMTQGGPLRSTTSI
                     VLYMYEEGFRWWRLGTASAVAVLLFLMTLAVTLVQLRWARNRAS"
     gene            265..1074
                     /locus_tag="VF720_16490"
     CDS             265..1074
                     /locus_tag="VF720_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017506094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX7881011.1"
                     /translation="MKRLLTYLALAIGGLLTLAPLLWMVSASFMATGETAILPPHLVP
                     DKPTLEHYRALFVRLHLARNFLNSALVTVIATVVSVFVNSMAAYAFAKLAFKGRDTLF
                     RSLTLALVIPAQVAMLPLFLILRTMGLVNTFAGVIIPYVAGVFSIFMVRQYLLGLPDE
                     LLHAARVDGAGEFRIYRSIVLPVITPILVTLAAFTFLSAWNDFMWPLIVLSDESRYTL
                     PVALANLSGEHVQDTELMMAGSVLTVLPALIVFLLFQKAYVRGILGGSVKG"
     gene            1071..>2065
                     /locus_tag="VF720_16495"
     CDS             1071..>2065
                     /locus_tag="VF720_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEX7881012.1"
                     /translation="MMKYSIRLFFVIVLQWLAPTPAAAADRQVLDDFEDVSAWAPFPA
                     DGVSLKLSSDTGRIGKALRMDFGFTGGGYAVARRKLDLDLPENYAFTFDIKGDAPVNH
                     LEFKLLDASGENVWWYVRRDMTFPADWTLLRTKKRQITFAWGPLPGELRHVAAVEIVV
                     TAGTGGTGTLWVDDFALVTLPPPGATPPQPVAEASTEQPNAAAGLAVDSDSLSAWRPA
                     ELPAGLTIDYAVPREFGALTLTWGAVGVPSEYAVLGSMDGRSWTTLASVYHGNAGRDD
                     FLLPESEWRYLRIDLPPPADPTSAPPLPSLLDVRLQPLEIGASREAFFGMLARAAP"
BASE COUNT          278 a          754 c          642 g          391 t
ORIGIN      
        1 acacgtcacc ctcccgggac tcgggccggt gctcctcttc gtggccgtca ccacgctcat
       61 cggcttcttc cagctcttct ccgagccgta cgtcatgacg cagggcgggc cgctgcgcag
      121 caccaccagc atcgtgctct acatgtacga ggaagggttc cgctggtggc ggctcgggac
      181 ggcctcggcg gtggcggtcc tcctgttcct catgacgctc gccgtcacgc tggtccagct
      241 ccgctgggcc cggaaccgcg cctcttgaaa cgcctcctca cctacctggc gctggcgatc
      301 ggcggtcttc tcacgctggc gccgcttctc tggatggtgt cggcctcatt catggccacc
      361 ggcgagaccg ccatcctgcc gccccacctc gtgccggaca agcccacgct cgagcactac
      421 cgggcgctct tcgtgcggct gcacctggcg cggaacttcc tgaacagcgc gctcgtcacg
      481 gtcatcgcca ccgtcgtgtc ggtcttcgtc aattcgatgg ccgcctacgc tttcgccaag
      541 ctggccttca agggtcgcga cacgctcttc aggagcctca cgctggccct ggtgattccc
      601 gcccaggtgg ccatgctccc cctcttcctc atcctgagaa cgatggggct ggtgaacacc
      661 ttcgcggggg tcatcatccc gtacgtggcc ggcgtcttca gcatcttcat ggtgcgccag
      721 tacctgttgg gcctgccgga cgaactcctt cacgccgccc gcgtggacgg tgccggggag
      781 ttccgcatct atcgttcgat cgtcctgccg gtcatcacgc cgatcctggt gacgctggcc
      841 gccttcacgt tcctctcggc ctggaacgac ttcatgtggc cgctgatcgt cctgtccgac
      901 gagagtcgct acacgctccc cgtggcgctg gccaacctgt cgggggagca cgtgcaggac
      961 acggagttga tgatggcggg atcggtgctc acggttctgc ccgcgctgat cgtcttcctg
     1021 ctgttccaga aggcctatgt tcgcgggatt ctcggcggga gcgtgaaggg atgatgaaat
     1081 actctatccg attgttcttc gtcatcgtac tccagtggct cgccccgacc cccgccgccg
     1141 cagccgatcg acaggtcctc gacgatttcg aggacgtctc cgcctgggcc cctttccccg
     1201 ccgacggcgt ctcgctcaag ttgagttcgg acaccggccg catcggcaag gccctgcgga
     1261 tggacttcgg cttcaccggc ggcggctacg ccgtggcccg ccgcaagctt gatctcgacc
     1321 tccccgagaa ctacgcgttc acgttcgaca tcaaggggga cgcgccggtg aatcacctcg
     1381 agttcaagct gctcgatgcc agcggcgaga acgtctggtg gtatgtgcgg cgcgacatga
     1441 cgttccccgc cgactggacg ctgctccgca cgaagaagcg gcagatcacc ttcgcctggg
     1501 gcccgcttcc cggggagctc aggcacgtgg ccgccgtcga gatcgtggtg acggccggca
     1561 ccggcggcac cggcacgctc tgggtggatg acttcgcgct ggtcaccctc ccgccgccgg
     1621 gtgccacgcc gccccagccg gtggccgaag cgtccaccga acagccgaac gccgcggccg
     1681 gcctggccgt cgactccgac tcgttgagcg cctggcgccc cgccgagctt ccggccggcc
     1741 tcacgatcga ctacgccgtc cccagggaat tcggcgcgct cacgctcacc tggggcgcgg
     1801 tgggcgtgcc gtcggagtac gccgtcctgg ggtcgatgga tggtcgctcc tggacgacgc
     1861 tggccagcgt gtatcacggc aatgccgggc gcgatgactt cctgctcccc gagtccgaat
     1921 ggcggtatct ccggatcgac cttcccccgc cggccgaccc gacatcggcg cctccactcc
     1981 ccagcctgct cgacgtccgg ttgcagccgc tcgaaatcgg ggccagtcgc gaggcgttct
     2041 tcggcatgct ggcccgcgcg gcgcc
//
LOCUS       DASVOD010000772         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_415614, whole genome shotgun sequence.
ACCESSION   DASVOD010000772 DASVOD010000000
VERSION     DASVOD010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415614"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VF720_16500"
     CDS             complement(<1..673)
                     /locus_tag="VF720_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881013.1"
                     /translation="MRTEPHRSGSAFFERCCRVILLLTCCLAFWVVRPAAASHINIVV
                     DGDLTDLIAGVNANLGGANGGFMASDPLGDIYTGPCAYVNGYDIRRTYALFDYRDDNG
                     DLTSTDVTLYLGWVVEGAIGDVDGNGNPNTFEITSPGGSSGCALSDENGIGPNESYNL
                     LFDLDCTGPVDDVRIAVKNNVVAIVSGANLIPLPGASFAFNGSSLEVKIPDYQNLLAP
                     LNLVGV"
     gene            complement(1000..2046)
                     /locus_tag="VF720_16505"
     CDS             complement(1000..2046)
                     /locus_tag="VF720_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7881014.1"
                     /translation="MTTDALISVRELAMHFPVGSAGLFGGGRRVLRALDGIDLDIVRG
                     ETLGLVGESGSGKSTLGRCLVRLYQPTRGRILHDGEDLAHLGGAAMRARRRLLSMIFQ
                     DPYASLNPRMTVEDIVGEPLAVHRLASGAARRAKVGELMELVGLDPRFIRRYPHEFSG
                     GQRQRIGIARALATGPEFIVADEPISALDVSIQAQILNLMSDVKSRLGLTTLFISHDL
                     AAIRHVSDRVAVLYLGKLVELGPAAAIEEQPCHPYTVALLSAAPSINPTRARRAILLP
                     GDPPSPLAPPSGCRFHPRCPLAFDRCRTEEPMLRPINTTTGVRLAACHVAEEVPSRLT
                     EFARSEGGSTTTFS"
     gene            complement(2043..>2434)
                     /locus_tag="VF720_16510"
     CDS             complement(2043..>2434)
                     /locus_tag="VF720_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX7881015.1"
                     /translation="VTHDLAVVAGMAQRVAVMYAGRIVELAPTASLFADPRHPYTIGL
                     LRSLPGRRRAGAAGGRLASIPGRPPDLAHLPAGCPFAPRCPWAFDRCHAERPPLRPLA
                     TGTQSACWLDDPARAPTTQPTLWEDRS"
BASE COUNT          434 a          762 c          836 g          402 t
ORIGIN      
        1 ccacgcccac caggttcagc ggggcgagca ggttctggta gtcggggatc ttcacttcga
       61 ggctcgagcc gttgaacgcg aaactggcgc ccggcagcgg gatcaggttg gcgccgctga
      121 cgatcgccac gacattgttc ttgaccgcga tgcggacgtc gtccacgggg cccgtgcagt
      181 cgaggtcgaa cagcaggttg tacgattcgt tcggcccgat gccgttctcg tcgctcagcg
      241 cgcacccgct cgagccgccg gggctggtga tctcgaacgt gttgggattg ccgttgccgt
      301 cgacgtcgcc gatggcacct tccacgaccc accccaggta gagagtgacg tccgtcgacg
      361 tgagatcacc gttatcgtcc cggtaatcga acagcgcgta cgtccggcgg atgtcatagc
      421 cgttcacgta ggcgcagggg ccggtgtaga tgtcccccag ggggtcggaa gccatgaagc
      481 cgccgttggc gccgccgaga ttggcgttga ctccggcgat caggtcggtg aggtcgccgt
      541 ccaccacgat gttgatgtgc gatgcggcgg ccggccgcac gacccagaag gcaagacaac
      601 aggtgagaag caggatgacg cgacaacagc gctcgaagaa agcgcttccg gaacggtgag
      661 gctcagttcg catgtgaccc cccgacagca agtgccatcg atctgaagaa cggatgaaaa
      721 cctggatgta tcgctcgatt gtttcgtgac gggaggaacg cggccgttgg ctcccgatga
      781 aaaacggaag ggccttcttt tcaagagcgt ggcgggtcac ctccggtcgg atggttgtgc
      841 ggcgtcgagt gcgatcaact gaggtgcgag ccacaggtta ttccagcggg aattcaattc
      901 gcaagaattt ccgaactttt caaaacaaat aagggattgc cctatcacaa cttgcagcgt
      961 ttttcgtcgt ctcgtccgtc ggtgtcgagt cgggaacgct tacgaaaacg tcgtggtcga
     1021 tccgccctca cttcgcgcga attcggtgag tcgtgacggc acctcctcgg ccacatggca
     1081 ggcggccagg cgtactcccg tagtggtatt gatcggtcgc aacatcggct cttcggttcg
     1141 gcaacgatca aaagcaagtg gacaacgagg atggaagcga caccccgatg gtggtgccaa
     1201 cgggctcggt ggatcgcccg gcaaaaggat ggcgcgacgg gctcgcgtcg ggttgatgct
     1261 tggcgcggcg gagaggagcg ccaccgtgta aggatgacac ggttgctcct cgatcgcggc
     1321 cgccggaccg agttcgacga gcttgccgag gtagaggacc gccacccggt cggatacgtg
     1381 acggatagcc gccaggtcgt ggctgatgaa gagcgtcgtc agaccgagcc ggctcttgac
     1441 gtcgctcatg aggttcagga tctgggcctg gatcgacacg tcgagcgccg agatcggttc
     1501 gtccgccacg atgaattcgg gcccggtggc cagggcccgc gcgattccga tccgctggcg
     1561 ctgcccgccg gaaaactcgt gcggataccg gcggatgaac cgtggatcga ggcccaccag
     1621 ctccatcagc tcgcccacct tcgctcgacg ggccgcgccg cttgccaggc gatgcaccgc
     1681 caggggctcg ccgacgatgt cttccaccgt catgcgcggg ttcaggctgg cgtagggatc
     1741 ctggaagatc atggagagaa gccgacgtcg tgcccgcatg gcggcacccc caaggtgggc
     1801 caggtcttcg ccgtcatgca ggatgcgtcc gcgcgtcggc tggtacagcc gcacgaggca
     1861 gcgtccgagc gtcgattttc ccgagccact ctcaccgacc agccccagcg tctcgccccg
     1921 cacgatgtcg aggtcgattc catccagcgc gcgcagcacg cggcgtccac cgccgaagag
     1981 gccggcgctg ccgaccggga agtgcatggc cagttcgcgg acgctgatga gcgcgtcggt
     2041 cgtcacgagc gatcctccca gagcgtcggc tgggtggttg gcgcgcgggc cggatcgtcg
     2101 agccagcagg ccgattgagt gccggtggcc aacgggcgaa gcggcgggcg ctccgcatgg
     2161 cagcggtcga acgcccatgg gcagcgcggc gcgaacggac accccgccgg caggtgcgcc
     2221 aggtcgggcg ggcgccccgg tatcgaggcc agtcgcccgc cggccgcccc ggctctgcga
     2281 cgtccgggga gcgaacgcag gaggccgatc gtgtagggat ggcgcggatc ggcaaagagc
     2341 gaggccgtcg gcgccagctc cacgatgcgg ccggcgtaca tcaccgccac ccgttgcgcc
     2401 atgccggcca ccaccgcgag gtcgtgcgtc acca
//
LOCUS       DASVOD010000773         5842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_886451, whole genome shotgun sequence.
ACCESSION   DASVOD010000773 DASVOD010000000
VERSION     DASVOD010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886451"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VF720_16515"
     CDS             <1..756
                     /locus_tag="VF720_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX7881016.1"
                     /translation="SGRKLSTDMARLDQLLGGGIELGSSTLIAGPPGTGKSSLAAGMV
                     AAAAARGEKASVFIFDEGVHMLLSRAAALGADLRPGLDKGLIKVQPIDPAELSPGEFA
                     DAVRRAAEEHGASIIVIDSLNGYLNAMPDERFLTIQLHEMLTYLAQKGVATIIIGAHQ
                     GLIGTTMSSPVDATYLADTVILLRYFEALGEVHQAISIVKKRGSEHERTIREFRLGRG
                     GIEIGEPLRDFRGVLTGVPTFDGKPTDLIRDWK"
     gene            759..2738
                     /locus_tag="VF720_16520"
     CDS             759..2738
                     /locus_tag="VF720_16520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7881017.1"
                     /translation="MPRPVTPLERRALIMAPTGRDAALTEAMLGRSNIGSAICRDMVD
                     FGSELARGAGVLIIAEEALSVDISRSLIQLLSGQPSWSDLPVLLLTRHGASSAIVAAA
                     TEDLGNVTLLERPVRTAAFLTAVRAALRARDRQYELRDRVEDQAMLAAIVTWSDDAMV
                     SKSLDGIIQTWNGAAERMFGYTEAEAVGQPITLIVPADRLAEERMIINRLLRGERLDH
                     FETLRVRKDGRLLDVSLTISPIYDATGRVVGASKVARDITHQKATEQALRDADRRKDE
                     FLATLAHELRNPLAPIRNSVHILKLLEKLPPEVEHLSSIMERQINHMVRLVDDLMEVS
                     RITRGKIDLRKEPVSLGSVLAGALETSQPLIDAANHRIHSVIPSTPMIVDGDPIRLAQ
                     VFSNLLNNAAKYTRPGGQIWLTVRADGSTAIVTIRDSGVGITREMLPRIFDLFAQDRS
                     AVDLSQGGLGIGLTLVKSLVHMHGGTVSVNSPGRGQGSEFVVRLPLIAVPSPPHPPAP
                     QMGSGLKQLRVLVVDDNKDATDSLSRLLQHLGAEVRAVHDGASALRALESFHPTLVLL
                     DIGMPEMDGYEVARHIRKLDPNKEMMLIALTGWGQDEDRRRSFRAGFDHHLVKPTDIA
                     ALQELINSVSGGGGGPSGGGGPAGGGKAPGRGLAP"
     gene            2882..3565
                     /locus_tag="VF720_16525"
     CDS             2882..3565
                     /locus_tag="VF720_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881018.1"
                     /translation="MSPAMYVHIAAGSVGLVSGLLSVVAPKGGSLHKRSGNVFTGAML
                     IMAGLGAGIAASRGQDSSILGGVLAIYLVVTAWTTVRPPTAQARTLDRAAMVYALGIT
                     LASLAMAADTMAAGKFARQGVPVPMLLIFGTVSLLGALGDWRMLKDGPPRGSRRIARH
                     LWRMCFAFFIATGSFFLGQADELPESFRIMPLLALFAFAPLLVMAYWMWRLSPKRKKP
                     APLQVSTAG"
BASE COUNT         1017 a         2067 c         1793 g          965 t
ORIGIN      
        1 tccggccgca agctctccac ggacatggcc cggctcgacc agttgctggg cggcggcatc
       61 gaactcggat cgagcacgct gatcgccggc ccgcccggca ccggcaagtc gtccctggca
      121 gcaggcatgg tggcggcggc ggccgcgcgc ggcgagaagg cctccgtgtt tatcttcgac
      181 gaaggcgtgc acatgctcct ttcgcgggcc gccgccctcg gcgccgacct gcgccccggc
      241 ctggacaagg ggctcatcaa ggtgcagccc atcgaccccg ccgaactgag cccgggcgag
      301 tttgccgatg ccgtcaggcg ggcggccgag gaacatggcg cgtcgatcat cgtgatcgac
      361 agcctgaacg gctatctcaa cgccatgccg gacgaacgtt tccttaccat ccagctccac
      421 gagatgctga cctacctggc ccagaagggg gtggccacca tcatcatcgg cgcgcaccag
      481 gggttgatcg gaacgacgat gtcctcgccg gtcgatgcca cctacctggc cgacaccgtc
      541 atcctgctcc gttacttcga ggccctgggt gaagtgcacc aagccatctc gatcgtcaag
      601 aagcgcggca gcgagcacga gcgcacgatc cgcgaattcc gcctcggaag ggggggcatc
      661 gagatcgggg agccgctgcg cgacttccgc ggggtactga cgggcgtccc cacgttcgac
      721 ggcaagccga cggacctgat cagggactgg aagtaacgat gccccgcccg gtcaccccac
      781 tcgagcggcg cgcactcatc atggccccga cggggcgtga tgccgcgctg accgaggcca
      841 tgctggggcg gtcgaacatc ggctcggcca tctgccgtga catggtggac ttcggcagcg
      901 aactggcacg cggggcgggc gtgctcatca tcgccgagga agcgctctcg gtggacatct
      961 cccgctcgct catccagctc ctgagcggcc agccttcctg gtcggacctg ccggtcctcc
     1021 tgctcacgcg gcatggcgcc agctcggcca tcgttgccgc ggccacggaa gacctgggca
     1081 acgtgacgct cctcgaacgc ccggtccgca ccgcggcgtt ccttaccgcc gtgcgcgccg
     1141 cgctccgggc ccgcgaccgg cagtacgagc tgcgcgaccg cgtggaggac caggccatgc
     1201 tggccgccat cgtcacctgg tccgacgacg ccatggtgtc caagtcgctc gacggcatca
     1261 tccagacctg gaatggcgcg gccgagagga tgttcggcta taccgaagcc gaggcggtcg
     1321 gccaaccgat cacgctcatc gtgcccgccg atcggttggc cgaggaaagg atgatcatca
     1381 accgcctgct gcgcggcgag cggctcgacc acttcgaaac cctgcgcgtg cggaaggacg
     1441 ggcgcctgct cgatgtgtcg ctgaccatct cgccgatcta cgacgccacc gggagggtgg
     1501 tgggcgcctc gaaggtggcc cgcgacatca cccaccagaa ggccaccgaa caggccctgc
     1561 gcgacgccga ccgccggaag gacgagttcc tggccacgct ggcccacgag ttgcggaacc
     1621 cgctggcccc catccgcaat tccgttcaca tcttgaagct gctcgagaaa cttccgcccg
     1681 aggtggagca cctctcgtcc atcatggagc ggcagatcaa tcacatggtg cgcctcgtgg
     1741 acgacctaat ggaggtgtcg cgcatcaccc gcggcaagat cgacctgcgc aaggagcccg
     1801 tgagcctggg atcggtgctg gccggtgccc tcgaaaccag ccagccactg atcgatgcgg
     1861 ccaaccaccg catccattcc gtcattccct cgacaccgat gatcgtggac ggcgatccca
     1921 tccgcctggc ccaggtcttc tcgaatctgt tgaacaacgc cgccaagtac acccgccccg
     1981 gcggccagat ctggctcacc gtgcgcgccg acggctcgac ggccatcgtg accatccgcg
     2041 acagtggtgt gggtatcacg agggagatgc tcccccgcat cttcgacctg ttcgcacagg
     2101 accgctccgc cgtggacctg agccagggag gcctcggcat cggcctcacg ctcgtgaaga
     2161 gcctcgtgca catgcatggc ggaaccgtca gcgtgaacag ccccggccgt gggcagggaa
     2221 gcgaattcgt ggtgcgcctg ccgctgatcg ccgtcccttc tcccccccac ccgcccgcgc
     2281 cacaaatggg cagcggcctc aagcagttgc gtgtgctggt ggtggacgac aacaaggacg
     2341 ccaccgacag cctgagccgg ctgctgcaac acctcggcgc ggaagtccgc gccgtgcatg
     2401 acggcgcctc ggccctgcgc gcgctcgagt cgtttcaccc aacgctggtc ctcctcgaca
     2461 tcggcatgcc cgagatggac gggtacgagg tggcccgcca catccgcaag ctcgatccca
     2521 acaaggaaat gatgctgatc gccctgaccg gctggggcca ggacgaagac cgcaggcgct
     2581 cgttccgcgc cggcttcgac caccacctgg tgaagccgac ggatattgcg gcgctgcagg
     2641 agctgatcaa ttcggtgagc gggggtggcg gcgggccttc gggcggtggc gggcccgcgg
     2701 gcggcgggaa ggctcccggc cgaggccttg ccccgtaacg cccggcccct ccgcgagtcc
     2761 ctcaaaccct taccgcgtca tcgccccaga ttccgcaacc cccttcgaga ctcccccgta
     2821 ctccacgccg gtcccgaccg gggccgctct gccatggccc gagcctcgtt ggagttcgcg
     2881 catgagtccc gccatgtacg tccacattgc cgccggctcg gtcggcctcg tctccggact
     2941 cctttccgtg gttgccccca agggggggtc acttcataag aggagcggaa acgtcttcac
     3001 cggtgccatg ctgatcatgg ccggactcgg cgccggcatc gccgcctccc gcggccagga
     3061 cagctccatc ctgggcggcg tgttggccat ctacctcgtc gtgaccgcct ggaccaccgt
     3121 ccgccccccc accgcccaag cccgtaccct cgaccgcgcc gcgatggtct acgccctcgg
     3181 catcaccctc gccagcctcg ccatggccgc cgatacgatg gccgccggaa aattcgcccg
     3241 ccagggcgtg cccgtgccga tgctgttgat cttcggcacc gtctcgctcc tcggagccct
     3301 tggcgactgg cggatgctga aggacggacc accccgcggc tcgaggcgaa tcgcccgtca
     3361 cctctggcgc atgtgcttcg cgttcttcat cgccaccggc tcattctttt tgggccaggc
     3421 cgacgaactc cccgaatcgt tccgcatcat gccgctgctc gcgctctttg cctttgcgcc
     3481 gctgttggtg atggcgtact ggatgtggcg cctgtcgccg aagcgaaaga agccggcgcc
     3541 actgcaggta tcgacggccg gataacgcct ccgccggcgg gtccgctccg gaggcggcct
     3601 gactccggag gcggcccggc tccgcaggct gctcgactcc gcaggccgcc tgactccgta
     3661 ggccgccacc ggctcccgca caaagccatc aaactcgcgc tccgcttcgt agcgattgtg
     3721 cgcccgacac cgcagctcga tgttcccgac gttggcccgc cccccgcgcg cgtacggcac
     3781 caggtggtgg aattccagcc acgcgcgctc gttgcagcgg tggcccgagt tggaacgaaa
     3841 cgcacatcga tcgtgatcgc gttcactgac cgcgcgccgt acgtcaaccg gaatgtaacg
     3901 cgatgggcgg cgagtttcgg cctgacgttc gcgacgctgc ctcacctccg atgtcccggc
     3961 cgacacttcc gccgccacct tcggcactgc cgcctttccg gtaaccgccc gcgggcgcgt
     4021 gccgatcgcc cgtttctcga cctggcgtcg caactgccgg agcgcgagca tcacgacggc
     4081 tgccggatcg ccgtcgggaa cctgatgacg catcaggtca atcagccgca gcaggtcgtc
     4141 atgcgcctcg gacgagatcg tcaggtggag ccggaagttg atggacgggg cacgatcgac
     4201 agtctcggga acaagagtat cgggccccgt ccccgccggg gtggcggggg ccgaaagttc
     4261 ggacgaatgc ttcctcgcag cgggaacgtc cggcgttgcc tcaagagtcg ggtgatcatc
     4321 gccagatgga ttgctcgaat cgcccctcag gaacgatgtc ggcgacggaa ccgccgcccg
     4381 ctgcgcaacg atccgcgtcc gaaccgcgcc acccggctgg aatcgcgcga cgatcgcatc
     4441 gattcccagg cgtgatgctc ccgccgcctc atcgagaagc cccgccaggt tttcaggagt
     4501 gacgtgtggc gccagccgcg caattgcatc caggtgaatg tcgccgctcg caatccgcgg
     4561 cccgatttcc gggaatcgcc gcaacgcgcg cgcggcccgg atgcgcacgt aggccgccga
     4621 ctcgctcagg cacagaaccg ttcggcacca gacgaagagg gatggacacc cttcggccag
     4681 gtacgcccgc cgacgatcga cttcggagag atgcgtcaac cattcgagga cgctcgcacg
     4741 ctcgcgcaca gtcagcagtt tcagttgcga tatcacttcg tcggtggtga gctcttccag
     4801 cccggatcgt ttcgttttca tgaaggaagt gtcgcatgcg gattttcgag tgaaggacat
     4861 gagccatggc cagacatcgg gcgtcgtcag gtttcaaatg attcaagtgt gcgattgcgt
     4921 tgaggagcgg agccgaacga tgcagacagg tctcgtcaaa ttgggcgccg cgagacttca
     4981 ccacgttcgg aaaaaccgtc gtcaacaaaa atatcgagca agcgaatttt cttgaactgt
     5041 ggctagccac agtcgaccgg ctattcctca aacagtgttc cgcaaaagga atgttgtcgc
     5101 tgttcccacc ttcgtcatcg acccgctcca ggcccatccc ctgtccgatg gagcggccgg
     5161 cggcgatagc ggagcaccgc cttctcaagt caacggccaa ttacgctgat aacttcctgt
     5221 cgggacgttc gttagggaaa cgtgaacccg tggggcgccg gaccatgcca tagaaacacc
     5281 ctgccggcct gccagatttc tccatcccgc acccaaataa ttgtggccga tcgcggtccg
     5341 ccagatgtaa attcagccga ggccaaccgg gccttctccc ccgtcatgac ccagaacgag
     5401 gcacacgatg gcgtcgtttc ccacaccccg ggttcatgag gtccaagcct ttaccccccg
     5461 cgaccaaacc tccccattcg tgccggccct ccaggacgcc aaggattgcc gcgcgtgcac
     5521 gaccaagggg acgtccctgt tccgggcgac cggcggtgat tcatgaaatt cgcccccaga
     5581 cactcgcgcc tcatccccat cctagccctg acgaccatcg gcctcttcgg gatcgactcc
     5641 caaattcgtg tcgcaacggc aaaggtgctg atcgaacagc cggataagca gacggatggg
     5701 gatccggagg acataactga gggagcccct cgccgcattt cgccgacagt ctcaacccca
     5761 ccaactgagc ctgtgcgcac aactccgcag cacttctgga caagagcact gaattggctc
     5821 cgatccaggc ttcggggttt ga
//
LOCUS       DASVOD010000774         2506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_676588, whole genome shotgun sequence.
ACCESSION   DASVOD010000774 DASVOD010000000
VERSION     DASVOD010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676588"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1968)
                     /locus_tag="VF720_16530"
     CDS             complement(<1..1968)
                     /locus_tag="VF720_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX7881019.1"
                     /translation="MPDARSEQPPAPTAGHDAIRLGVPGFEFHDLFRPYRLVDLDRAF
                     LNHVREHDAPLVEQLIAARADGGASLDEPATVEMLMHLAPHVGRFIARLFDIEDAAAN
                     QDDKAIEDAPIYDCRRLFLERRVFKSIPDEVTLLATNLAAAEAAYRDVIDRRLPPAAG
                     TDDPELELGRIAVLLMQVETTARTESPEVLAGVQADLDTVGAWATLLAYHPEFRGTSG
                     RWTIFFRPHKLDFEELVEREFDDPAVPTLFHGAHHKRRRRDGFDLTDRRGTRRQALSE
                     MHYCIICHERKKDSCSHGFPEAAPTTETPTTVEAETTPAARRVHATDTPKLGRAFKKN
                     PLGIPITGCPLGEKISEAHALKRGGHGLAALAMIMIDNPMCAGTGHRICNDCMKGCIY
                     QKQTPVDIPMAETNILTGVLGLPWGFEIYTLLMRWNPLNLRRPVALPYNGKNVLVVGM
                     GPAGYTLAHYLLNEGFAVVGIDGFKIEPLAFRFLGDALGTPRPIKDMRKHLMPLTDRT
                     VLGFGGVSEYGITVRWDKHFLDLNYHALMRRDHFLLYDGVRFGGTLTIEDAWEQGFHH
                     IALAAGAGRPTMVDAPGSLLRGIRSASDFLMTLQGGGAYKNTSLANLQIHLPALVIGG
                     GLTAVDAATETSAYYVAQVERTLERYQALVAD"
     gene            complement(1989..>2506)
                     /locus_tag="VF720_16535"
     CDS             complement(1989..>2506)
                     /locus_tag="VF720_16535"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX7881020.1"
                     /translation="TSTAQYGVLVVGQGSVELSNCSISSTREAALALTETGRATGLNV
                     TLSGANFGVITDGSSSATLTGPKITGNGIGAQARAGSTIRLTGGQVARSRQSGLLVTN
                     GGTIQATGTAIEQNGGNGATARSGKITLNGVTISNNAQFGALCESAAGVIEKIGVTFV
                     GNKMGDTLGCP"
BASE COUNT          381 a          797 c          899 g          429 t
ORIGIN      
        1 gtcggcaacg agcgcctggt agcgctcgag ggtgcgctcc acctgcgcca cgtagtaggc
       61 ggaggtttcg gtggcggcgt ccacggcggt caggccgccg ccgatcacga gcgccggcag
      121 gtggatctgc agattggcga ggctcgtgtt cttgtacgcg ccaccaccct gcagcgtcat
      181 caggaaatcg gaagccgagc ggatgccgcg gaggaggctt cccggggcgt cgaccatcgt
      241 ggggcgcccc gcgccggcgg ccagggcgat gtgatggaac ccctgctccc aggcatcctc
      301 gatcgtgagc gtgccgccga agcggacgcc gtcatagagc aggaagtggt cgcgccgcat
      361 cagcgcgtga taattcaggt cgaggaagtg cttgtcccag cggacggtga tgccatattc
      421 ggacacgccg ccgaaaccga gcacggtacg atcggtgagg ggcatgaggt gcttccgcat
      481 gtccttgatg gggcgcggcg tgccgagcgc atcgccaagg aaacggaagg ccagcggctc
      541 gatcttgaag ccgtcgatcc ccaccacggc aaacccctcg ttgaggagat agtgcgccag
      601 cgtgtagccg gccggcccca ttcccaccac caggacattc ttgccgttgt aggggagggc
      661 cacggggcga cgcaggttga gcgggttcca acgcatcagc agcgtgtaga tctcgaagcc
      721 ccacggcagc cccaggacgc cggtcaggat gttggtttcc gccatcggga tgtcgaccgg
      781 cgtctgcttc tggtagatgc atcccttcat gcagtcgttg cagatgcggt gaccggtgcc
      841 ggcgcacatg ggattgtcga tcatgatcat ggccagggcc gccagcccgt ggccgccccg
      901 cttgagtgcg tgcgcctcgg agatcttctc gccgagcggg cagccggtga tcggaatgcc
      961 gagcggattc ttcttgaagg cgcggccgag cttgggtgtg tcggtcgcgt gcactctcct
     1021 ggccgccggc gtcgtttccg cctccaccgt ggtgggcgtc tcggtcgtcg gggccgcttc
     1081 cggaaatccg tggctgcacg agtccttctt ccgttcgtgg cagatgatgc agtagtgcat
     1141 ctccgagagc gcctgccggc gggtgccccg gcgatcggtc aggtcgaagc cgtcgcgccg
     1201 gcgtcgcttg tggtgcgccc cgtgaaaaag cgtgggaaca gcgggatcgt cgaactcccg
     1261 ctccaccagc tcctcgaagt cgagcttgtg gggccggaag aagatcgtcc agcggccgct
     1321 cgtgccgcgg aattcggggt gataggcgag gagcgtggcc caggcgccca cggtgtcgag
     1381 gtcggcctgc acgcccgcca acacctccgg cgattcggtc ctggcggtgg tttccacctg
     1441 catgaggagg acggcaatcc gcccgagttc aagctcggga tcgtccgtgc cggctgccgg
     1501 cggaagccgg cggtcgatca cgtcgcgata ggccgcttcg gctgcggcca ggttcgtcgc
     1561 aagaagtgtg acttcgtccg ggatcgactt gaagacccgg cgctcgagaa acaggcgacg
     1621 gcagtcgtag atcggcgcgt cttcgatcgc cttgtcgtcc tgattggcgg cggcgtcctc
     1681 gatgtcaaag aggcgcgcga tgaagcgccc cacgtgcggc gccaggtgca tcagcatttc
     1741 gacggtggcg ggttcgtcca gcgaggcgcc cccgtcggcg cgcgcggcga tcagctgttc
     1801 gacgaggggc gcgtcgtgct cacggacgtg gttcaggaag gcccggtcga ggtccacgag
     1861 ccggtagggc cggaaaaggt cgtggaattc gaacccgggc acaccgagac ggatggcgtc
     1921 gtgaccggcc gtcggggcgg gcggctgttc ggagcgggca tccggcacgg cgcgaacctc
     1981 cagcggctct atgggcagcc cagcgtgtcg cccatcttgt taccgacgaa ggtgacgccg
     2041 atcttctcga tgacgcccgc ggccgattcg cacagggcgc cgaactgcgc gttgttcgaa
     2101 atcgtcacgc cgttgagcgt gatcttgccc gaacgcgccg tggcgccgtt gccgccgttc
     2161 tgctcgatgg cggtgccggt agcctggatc gtgccgccat tggtcacgag gagcccgctc
     2221 tgccggctgc gggccacctg tccgccggtc aggcggatcg tggatccggc ccgcgcctgc
     2281 gcgccgatcc cgttgccggt aatctttggc ccggtcagcg tggccgagct cgaaccgtcg
     2341 gtgatgacgc cgaagttggc gccggagagc gtcacgttga ggccggtggc acgaccggtt
     2401 tcggtcaggg ccagcgccgc ctcccgcgtg ctggagatgg agcagttcga cagctccacc
     2461 gagccctggc cgaccaccag caccccgtac tgggccgtgg aggtga
//
LOCUS       DASVOD010000775        11904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_624780, whole genome shotgun sequence.
ACCESSION   DASVOD010000775 DASVOD010000000
VERSION     DASVOD010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624780"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF720_16540"
     CDS             complement(<1..217)
                     /locus_tag="VF720_16540"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX7881021.1"
                     /translation="MPVSPDYRDFILEQLNRLAPTTGRAMFGGVGLYVDGVIVGLIDD
                     DVLYFKVNDATRGIFKAAGGHPFRPFGD"
     gene            305..1090
                     /locus_tag="VF720_16545"
     CDS             305..1090
                     /locus_tag="VF720_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881022.1"
                     /translation="MAAPRVSTVLILPLLLALCAPVSAGLLGIDSNYVLHDINTVNGV
                     TSNPRSVGNKVNMIAISPGGILYGVSQGFINDGPPGQQLFTINIATGAPTLVDTLSQP
                     IIVEGDIACDPTTGILYGVDSTGELYTINTTSALVVQVGTIGTNTDLSAATFDASGTL
                     WVVDSFGPTLLKVNKANGAVLMTVPLDPINQEVGGLAFSPAGTLFHAAGTTGKLYTLN
                     TTTGQALTIGPLAPSGGIWGLAWTPEPVSLEPSSWSRIKALVP"
     gene            1115..1201
                     /locus_tag="VF720_16550"
     tRNA            1115..1201
                     /locus_tag="VF720_16550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1149..1151,aa:Leu,seq:taa)
     gene            complement(1218..2069)
                     /locus_tag="VF720_16555"
     CDS             complement(1218..2069)
                     /locus_tag="VF720_16555"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX7881023.1"
                     /translation="MNRTIVCSLAFLGLAGVFSPDAAECRAATPPDSSRTEFLAVTLN
                     IWHDAENWPARMAVIRDTLRALHPDVVLLEEVLQNRVLPNQAATLAESLGYAWTFVSV
                     DPPEAAKRYGNAILTRHRIVETHEVKLAPLNDYRVAGHARLQVGNRSLDVFVTHLHHT
                     EEGDSIRVTQVDGLLAFVDTTRVPGGALLVGGDFNATPDAAEIKPVRDRLIDCWSLVH
                     PGKPDTTITTLNQAKGLPARRIDYLFLGSEYLRTVASEIFLDSPTVDGTWASDHFGVW
                     ARFEWVE"
     gene            complement(2192..4660)
                     /locus_tag="VF720_16560"
     CDS             complement(2192..4660)
                     /locus_tag="VF720_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881024.1"
                     /translation="MPACIARRVLFAMPLITLLAAEATAQSLRNDFWVAYPSANSLNP
                     VGVAAIVPTDSVVFIGGDFTTIGPPTGSAVAVDMATHEPLTVYPQVDGPVYAAIPDGV
                     GGYYIGGAFSTVQGQNRSGLARIGADGRVLPWSPGVKGSVRTIVTTGGKMIVGGSFSS
                     LGGVAASNLGAVDLGTGNGLPGWGPGTNGMVRALKLSGGLVYVGGSFTDISGLPSRDY
                     LIALETTSGVGQAWDPPMSEEVHSLAVSGNTVYAGGAFTFYGPSDTDGIVAIDADTGA
                     IRDWTTFIYSSGGGAIDVNTILVRNGAIYIGGKFSHVSSQLRFGVAALDSITGQPTAW
                     LPQAANIQGITESNGLLYLAGPTGVSAVDVATGAGTGWSVTTNTTAYGFVPLGTRGVV
                     YGAFTSAGCITRNRLAALSMQTGRPTDWNPNVNGPVYCMRLNGTTLYIGGRFEMIGGV
                     THNGAASFDATNGQLTSWTPSIGTALGGVKCMTIGAGGVVHIGGNFTSVSGSSRLNLA
                     AIDPATGLATPWNPSANGEVNALWYRPAFDAVPSTVWIGGAFTTVGGQGRLRLASVDA
                     AAGSAGGLLSWNPGAGNTVRTLLVLGNPPLTRIIAGGDFNTLAGQPRNYVGSMDQSGA
                     ATSWNPSADGPVHALTVTGTNYYISGTFTTLGGAPRSNVGIVGSNGLATTWNMPLVTG
                     SVRSMALRNNFLYAGGLFLGVQGWPHSGFAAITQGATTGVGDDEEAPKAPVVVTAWPN
                     PFTRRVTLRVDDGQNSRLTASIFDIRGRLVARLGDEAFDAARRELSWDGHDLGGRDTP
                     SGVYFARLHGGSAHRSVRLVRVVE"
     gene            4963..5220
                     /locus_tag="VF720_16565"
     CDS             4963..5220
                     /locus_tag="VF720_16565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881025.1"
                     /translation="MLMQRLKTAAWTAMGTAMIALPALAQQGQPGPSGPPGPQGPPGP
                     SGTTTTILGVDQSTALLIGGLVLFVVVVLAIVSMANRDRTA"
     gene            complement(5283..6293)
                     /locus_tag="VF720_16570"
     CDS             complement(5283..6293)
                     /locus_tag="VF720_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012625580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEX7881026.1"
                     /translation="MPDLGGFGPALIILIVLALAFDFINGFHDTANAVATVISTRVMR
                     PWTAIALAGVLNFLGALSGTEVAKTIGSGIVGASVPTGAIIAALAGAIAWNLLTWYYG
                     IPSSSSHALIGSLLGAGIASLGFGSVHWNVLRDKVVLPLFLSPAAGFLIALFLMKLLI
                     RIFANMPPARVGPLFRRTQIVSASLMAFSHGSNDAQKTMGIITLGLLSAGVIHEFHVP
                     AWVLMSCAVAMGLGTLAGGRRIIHTMGTRFAHLEPIHGFAAESSAAAVIQVASHLGSP
                     LSTTHVISSCILGVGAARRLNAVRWGVARTMVSAWFLTIPISAALGFVVAFLERAIFG
                     PG"
     gene            complement(6286..6900)
                     /locus_tag="VF720_16575"
     CDS             complement(6286..6900)
                     /locus_tag="VF720_16575"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX7881027.1"
                     /translation="MSRKPNGFFENFEQAAANNIAAARLLEGLCSDFTNSEDRVKELH
                     DLEHRGDEISHDIYAALNKVFIPPLDREDIIEITRALDDVMDHIYGAADAMCVYNIKQ
                     PTAVAANFSKIIVSCTEAVAKQLPKLRQRKRMKEISEGIIEIHRLENEADVLLRQGLT
                     ELFHKPNDPVMVIAWSRIYETMERVTDKCEDIADVLRGLAIKHA"
     gene            complement(6953..7861)
                     /locus_tag="VF720_16580"
     CDS             complement(6953..7861)
                     /locus_tag="VF720_16580"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX7881028.1"
                     /translation="MSKPVLVTGASTGIGRAIVERLAAAGLPVYATARKPDDLAGLDA
                     IPDVMALPLDVRDPASVVALAETIRTAGRGLYAVVNNAGLGGIGPVASFTDEAVRDLF
                     EVNVFGVFRLTRACLPLILESHGRVIVIGSQGGSVSMRWYAPYTMTKHAMEAFTKALD
                     DEIRPHGARALIVQPGAVSTAIGDNSETGDRERFRSAPPPFDVDAAAILKGFDEPSVF
                     DPTQPESAGNRRHSPPDDVARVVESLLTDPDPPLRSLVGTRWEGNRVITTLIDRLVEA
                     NACPSLGYSADELAAKVRERMARGRP"
     gene            complement(7889..8770)
                     /locus_tag="VF720_16585"
     CDS             complement(7889..8770)
                     /locus_tag="VF720_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX7881029.1"
                     /translation="MLIVGAGAVGQVFGHYMALGGASVGVSVRPHRVDEARRFTLYRV
                     TGRDRTDTGVFRPITVVSTPAEVAGHSWDQIWLCVPSDALDRSWLESLIAASGSTTIV
                     GLTPGLHDAARLREWFPSHRHVDGVIGFMAWHSPLPGEARGPGTSVWLPPLSASFFDG
                     PDAGSIVNVLRSGGLPAQVKRGAAGLGAQASALMLPVIGALGEAGWSLRDLIHGRRLA
                     PAMASARHALRVVDRVHGTNSAFLRFIARAWLARLALGLAPRIVPFPFEAYLRVHFTK
                     VGSQVTAMLRDYGAPLS"
     gene            9068..9946
                     /locus_tag="VF720_16590"
     CDS             9068..9946
                     /locus_tag="VF720_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881030.1"
                     /translation="MKKAVWISLAAVVVLGMSAWVYTLSKEKKTVEGQYAQAVVTGDS
                     LRTSFDVALSSIAEIQDSLLAILPSESSVMHMSDQVETGGPMTASRKDQVLRSISDLQ
                     GSIQRSKEMIAGLESRLGEQDVKIGSLEKIISGLKRTVSDREHMIAGLNNKVESLTVE
                     VTNLKTDVAAGQAQIATQQQVIEDKRREISTVYYVVDTKDNLKDAGVVKSTGGVIGIG
                     KSTKLTGQFPQQVFARVDTDMQTTIPLSGKKPVVLSGQSATSYEIVPVNEQMSELRIV
                     NPEEFRKVRYLVVQVG"
     gene            10072..11040
                     /locus_tag="VF720_16595"
     CDS             10072..11040
                     /locus_tag="VF720_16595"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881031.1"
                     /translation="MLRMTRSSFLLRFLFPALVIPAVQVSAANALGPWSPGVVDHWGS
                     EGTGPGQFGLAHGLAVGPDGSVYVGDTLNNRVQKFTADGDYVLEFAVAFPGGVDVDDA
                     GNVFVVGGTDQVFKFSSTGEPITSWGGTGSDPGQFRFPLDVAVDSQGFVYVVDWMNHR
                     VQKFTNDGGFVLAFGSEGTGDGQFMTPVSIVCTGSDVITVHVVDMGLDRTVAFLGNGE
                     WTGGLPGDLHDPTGIALEHTGYLLLLDAGSGRIHVYGPGGEFQFAFGSEGNELGQFFH
                     PSAIDAGPDGRIYVMDKDNHRVQVFQSIASPIVPATWSGIKSLSAR"
     gene            complement(11018..11737)
                     /locus_tag="VF720_16600"
     CDS             complement(11018..11737)
                     /locus_tag="VF720_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7881032.1"
                     /translation="MITITGLSKSYGAVRALSDVSFTLAPGQICGLLGRNGAGKSTLI
                     RILVGLLPPDAGSLQVDGKPVPFGDITLRRAIGYVPESELLDDFLTVRQFLEFVAAIR
                     ELPIAERRADIDRWLAAFRLTDKADALLVECSHGMRRKTSMAGALLGRPRLLLLDEAL
                     NGLDPESRVVMRGVLRDFAAGGGTALFSTHVLETAETICDRALIIVRGKIARDLPSDA
                     WRGAPGTLEREFLAVSEPTAT"
BASE COUNT         2094 a         3819 c         4072 g         1919 t
ORIGIN      
        1 cgtcgccgaa gggccggaag ggatgtccac ccgcggcctt aaagatgccc cgcgtcgcat
       61 cgttgacctt gaagtagagg acgtcgtcgt cgatcaaccc gacgatcacg ccgtcgacat
      121 acaggccgac gccgccgaac atcgcccgcc ccgtcgtcgg cgcgaggcgg ttcagctgct
      181 cgaggatgaa gtcgcgataa tccggtgaaa cgggcatggg gccggaatcc tagcaccggg
      241 gccggaaggg tgggtgctac aatgtcggcc tgcccgtctg aaccacactc gccggaggta
      301 caccatggcc gcgcctcgtg tctccaccgt cctcatcctc ccgctgctcc tggctctttg
      361 cgcaccggtt tccgccggat tgctcggcat cgactcgaac tacgtcctcc acgacatcaa
      421 taccgtcaac ggcgtcacca gcaacccgcg ttcggtgggg aacaaggtga acatgatcgc
      481 catctcgccg ggcggcatcc tgtatggcgt ctcgcaggga ttcatcaacg acggcccgcc
      541 cggccagcag ctcttcacga tcaacatcgc gaccggcgcc cctacgctgg tagacacgct
      601 gagccagccc atcatcgtgg aaggggacat cgcctgcgac ccgacgacgg gcatcctgta
      661 cggcgtggat tcgaccggcg agctctacac gatcaacacg acgagcgccc tggtcgtgca
      721 ggtggggacg atcggcacga acaccgacct gtcggccgcg acgttcgacg cctccggcac
      781 cctgtgggtc gtcgactcgt tcggcccgac gctgctcaag gtcaacaaag cgaacggcgc
      841 cgtgctgatg accgtgccgc tcgaccccat caaccaggaa gtcggcgggc tggccttctc
      901 gcccgcgggc acgctctttc acgccgccgg cacgaccgga aagctctaca ccctgaacac
      961 gacaaccggc caggccctga ccatcgggcc gctggccccg tcgggcggca tctggggcct
     1021 cgcctggacg cccgagcccg tgtccctcga accgtcgagc tggagtcgca tcaaggcgct
     1081 ggtaccctga ccgtgttacc gtgccccacc ccgcgccggg gtggcgaaat tggcagacgc
     1141 tgcggactta aaatccgctg accgcaaggt cgtccgggtt cgaggcccgg ccccggcacc
     1201 aggtctacag gtcggcctta ttccacccac tcgaaccttg cccagactcc gaagtgatcc
     1261 gaggcccagg tgccatcgac cgtcggagag tcgaggaaga tctccgaggc cacagttcgc
     1321 aggtattccg agccaaggaa gaggtagtcg attcgccgcg ccggcaggcc ctttgcctgg
     1381 ttcaatgtcg tgatcgttgt atcaggcttg ccggggtgga caagcgacca gcaatcgatc
     1441 aggcgatccc gaacaggctt gatctctgcc gcgtcaggcg tcgcgttgaa gtcgccgccg
     1501 acgagaaggg ctccgcccgg aacccgcgtc gtatcaacga aggcgagaag gccgtcgacc
     1561 tgggtcaccc ggatgctgtc gccttcctcc gtgtggtgaa ggtgggtcac gaagacgtcc
     1621 agggatcgat tgccgacctg gagccgggcg tgaccggcaa cccgataatc attgaggggc
     1681 gccagtttca cctcatgcgt ttcgacgatc cggtgtcggg tgagaatcgc gttcccatac
     1741 cgcttcgcgg cttccggagg gtcgacggag acgaacgtcc atgcgtaacc caggctctcg
     1801 gccagcgtgg ctgcctggtt ggggaggacc ctgttctgaa ggacttcctc aagaagcacc
     1861 acgtccgggt ggagcgctct gagtgtgtcc cggatgacgg ccatccgcgc cggccagttc
     1921 tccgcgtcat gccagatgtt gagcgttacg gcgaggaatt cggtgcggct ggagtccggc
     1981 ggagtggcgg cccggcactc ggcggcgtca ggagagaaca ccccggcgag gccgaggaaa
     2041 gcgagcgaac atacgatcgt ccggttcatg ggccaagcgt aggtcggtcc cggagattaa
     2101 tccagaggat tgggggggac ctgtgatcgc caccgagcgc atcgagcctc cggcaagtgg
     2161 gtcagcgaac ggccggggag gtgatgctcc gtcactccac gacacgcacc aggcggacgc
     2221 ttcgatgcgc cgagcctccg tgaaggcggg caaagtatac gccactcggc gtgtcacgtc
     2281 cgccgagatc gtggccatcc cacgatagct cacgccgcgc ggcatcgaag gcctcatcac
     2341 caagccgcgc caccagccgc ccccggatgt cgaagatgct ggccgtgagg cgcgaattct
     2401 gcccgtcatc cacccgcagc gtcacacgcc gcgtaaaggg attaggccac gccgtcacga
     2461 ctacaggtgc cttcggcgcc tcctcgtcat cgcccacgcc ggtagtggcg ccctgggtga
     2521 tcgccgcgaa gccggagtgc ggccacccct gcacaccgag gaacagcccg cctgcgtaga
     2581 ggaaattgtt gcggagcgcc atgctgcgca cggagccggt caccagcggc atgttccagg
     2641 tcgtggccag gccgttgctc ccgacgattc cgacgttgct tcgcggggcg cccccgagcg
     2701 tcgtgaaggt gccggagatg tagtagttgg tgccggtgac ggtcagggca tggacgggtc
     2761 cgtcggcgga agggttccag gaggtggcag cgccggattg gtccatcgag ccgacgtagt
     2821 tgcgcggctg gccggccagc gtgttgaagt cgccgccggc gatgatgcgg gtgaggggcg
     2881 ggttgccgag gacgagcagc gtgcggacgg tgttgccggc tccggggttc catgagagga
     2941 gcccgccggc ggagcccgca gccgcgtcga ccgacgccag gcgaagtcgt ccctggccgc
     3001 cgacggtcgt gaaggcgccg ccgatccaga cggtgctggg aacggcatcg aaggccggcc
     3061 ggtaccagag ggcattcacc tcgccgttgg cgctcggatt ccagggggtg gccaggccgg
     3121 tggcaggatc gatcgcggcc aggttcaagc ggctggagcc gctgaccgag gtgaaattgc
     3181 cgccgatgtg cacgacgcca ccggcgccga tcgtcatgca cttgacgccg cccaaggccg
     3241 tgccgatgct gggagtccag ctcgtgagct ggccgttcgt ggcgtcgaag gaggccgcgc
     3301 cgttgtgcgt gacgccgccg atcatctcga agcggccgcc gatgtagagc gtggtgccgt
     3361 tgagtcgcat gcagtagacc ggcccgttca cgttgggatt ccagtcggtg gggcgcccgg
     3421 tctgcatgct gagggcggcc aggcggttgc gcgtgatgca acccgcgctg gtgaaggcgc
     3481 cgtacacgac gccgcgcgtg ccgagcggga cgaagccgta ggccgtggtg ttggtggtca
     3541 cgctccatcc ggtgcccgct ccggtcgcga cgtcgacggc agagacgccg gtgggcccgg
     3601 cgagatagag gagcccgttc gactcggtga ttccctggat attggccgcc tggggaagcc
     3661 aggccgtcgg ctggcccgtg atggagtcca gggcggcgac gccaaagcgc aactggctgg
     3721 agacatggct gaacttgccg ccgatgtaga tggcgccgtt gcggaccagg atggtattca
     3781 cgtcgatggc gccgccgccg ctggagtaga tgaacgtggt ccagtcgcgg atggcaccgg
     3841 tgtcggcatc gatggccacg atgccatccg tatcgctggg accgtagaac gtgaaggcac
     3901 cgcccgcgta tacggtgttg ccgctcacgg ccagcgagtg gacttcctcg gacatcggag
     3961 gatcccaggc ctgcccgacg ccactggtgg tctccagggc aatgaggtag tcgcggctgg
     4021 gcagtccgct gatgtccgtg aacgagccgc cgacgtagac gagcccgccg gagagcttca
     4081 gcgcgcgaac cattccgttc gtgcccggac cccagccggg caagccgttc ccggtgccga
     4141 gatcgacggc gcccaggttg ctggcggcga caccgccgag cgacgaaaac gagccaccca
     4201 cgatcatctt cccgcccgtc gtcacgatcg tgcggaccga tcccttcacc cccggactcc
     4261 acggcaggac gcgcccgtcg gccccgatgc gagcgaggcc ggatcggttc tgcccttgca
     4321 cggtgctgaa agcgccaccg atgtagtagc cgccgactcc gtcggggatg gcggcgtaga
     4381 ccgggccatc gacctgggga tacacggtga gcggctcatg ggtggccatg tcgaccgcca
     4441 cggccgaacc ggtgggaggg ccgatcgtgg tgaaatcgcc gccgatgaag acgaccgagt
     4501 cggtgggcac gatggcagcc acgccgaccg gattgagcga gttggcggag ggatatgcca
     4561 cccagaagtc gttgcggagg ctctgggcgg tcgcttccgc agccagcagg gtgatcaggg
     4621 gcatggcgaa caggacccga cgggcaatgc aggcaggcat gatgatcctc tcggggttgg
     4681 gacggtgctg ccgggatcag cgccgaaacc ggagaaagag gctcacggaa agggagtaca
     4741 agtttcccgg gcagcttcct atttgccccg atgtgcggac gtgtcgcgcc gccaatagat
     4801 tctgtatctt gttgatattt aatagtttgt ggcgatgttt gagcgaagtg gcatcccctt
     4861 tgcattttcc acgggccata cgccccgatg ttcagcacac cgcgggcata ccgaaaaacc
     4921 tcttcgacgg tccatcgacc gtcccggcaa aggagtacga ccatgttgat gcaacgactc
     4981 aagaccgccg cctggacggc gatgggaact gccatgatcg cccttcccgc actggcccag
     5041 cagggacagc ccggcccatc gggcccccca ggcccccagg gacctccggg cccgagcgga
     5101 acgaccacga cgatccttgg agttgaccag tcgaccgccc tcctgatcgg cggcctcgtg
     5161 ctcttcgtgg tggtggtcct ggccatcgtg tcgatggcga accgcgatcg cacggcgtag
     5221 cgcggaagac gacgacatca gatcggcaaa cggaagcggc cccgagatcg gggccgcttt
     5281 ttttatcccg gcccgaagat ggccctctcg aggaacgcca ccacgaatcc cagcgccgcg
     5341 ctgatcggga tcgtgagaaa ccacgcgctg accatcgtgc gggccactcc ccagcgaacg
     5401 gcgttcaggc ggcgggcggc gccgactccg aggatgcacg agctgatgac gtgggtggtg
     5461 ctgaggggtg agccgaggtg cgaggccacc tggatgacgg cggccgccga ggattccgcg
     5521 gcgaatccgt gaatcggctc caggtgggcg aatcgcgtgc ccatggtgtg gatgatccgt
     5581 cggccgcccg ccagcgtgcc cagtcccatg gccaccgcgc acgacatgag gacccaggcg
     5641 ggcacgtgga attcgtggat gacgccggcc gacaggaggc caagggtgat gatccccatc
     5701 gtcttctgcg catcgttgct gccgtggctg aacgccatga gcgaagccga tacgatctgc
     5761 gtccgtcgga agagcgggcc caccctggcc ggcggcatgt tggcgaagat gcgaatgagc
     5821 agcttcatca ggaagagcgc gatgaggaag ccggcggccg gcgagagaaa gaggggaagc
     5881 acgaccttgt cccgcagcac gttccagtgg acggagccga aaccgagact ggcgataccg
     5941 gcgccgagga ggctgccgat cagcgcgtgg ctggagctgc tcgggatgcc gtaataccag
     6001 gtgagcagat tccaggcgat ggcgccggcc agcgccgcga tgatggctcc ggtcggcacc
     6061 gacgcgccca cgatgccgct gccgatggtc ttggccactt cggtgccgga gagtgcgccc
     6121 aggaagttca ggacgccggc cagcgcgatg gcggtccatg gccgcatcac acgcgtggaa
     6181 atgaccgtgg ccacggcgtt ggccgtgtcg tggaacccgt tgatgaagtc gaacgccagc
     6241 gccaggacga tgagaatgat gagcgcgggg ccgaaaccgc cgaggtcagg catgcttgat
     6301 ggccaggccg cgcagcacgt cggcaatgtc ctcacatttg tccgtcacgc gctccatcgt
     6361 ttcgtagatg cgactccacg cgatgaccat gaccggatcg ttcggtttgt ggaacagctc
     6421 ggtgagtccc tgccgcagca gcacgtcagc ctcgttctcg agacggtgaa tctcgatgat
     6481 tccctccgag atctccttca tgcgcttgcg ctggcggagc ttggggagtt gcttcgccac
     6541 cgcctcggtg cacgacacga tgatcttcga gaagttcgcc gctactgccg ttggctgctt
     6601 gatgttgtaa acgcacatgg cgtcggccgc gccgtagatg tgatccatca cgtcgtcgag
     6661 cgcccgcgtg atctcgatga tgtcttcgcg gtccaggggg ggtatgaaca ccttgttcag
     6721 cgccgcgtag atgtcgtggg agatctcgtc gccccgatgc tcgaggtcgt gcagttcctt
     6781 gactcgatcc tcggaattcg tgaagtccga gcaaagccct tcgagcagcc ttgctgcggc
     6841 gatgttgttg gcggccgcct gctcgaaatt ttcgaagaac ccgttcggtt ttcggctcac
     6901 gtggttgtct ccttccaggg tccgacgata acggtaaccg ggcgcgctgt cgtcagggcc
     6961 ggccccgtgc cattcgctcg cggactttgg ccgccagttc gtcggcggag taccccagtg
     7021 aggggcaggc attggcttcc accaggcggt cgatcagcgt cgtgatgacc cgatttccct
     7081 cccagcgggt gcccaccagc gagcgcagcg gcggatcggg gtcggtgagc aatgattcga
     7141 ccacccgggc cacgtcgtcc ggcggagagt gcctgcggtt gccggccgac tccggttgcg
     7201 tggggtcgaa gacggagggc tcgtcgaatc ccttcaggat ggcggccgca tccacgtcga
     7261 atgggggtgg ggcgctgcgg aagcgctcac ggtcgcccgt ttccgagttg tcgccgatgg
     7321 cggtgctgac ggcgcccggc tgcacgatca gggcccgggc cccgtgcgga cggatttcat
     7381 catcgagcgc cttcgtgaac gcctccatgg catgcttggt catcgtgtag ggtgcgtacc
     7441 agcgcatgga gaccgaacct ccctgcgagc cgatgacgat gacgcggccg tgcgactcga
     7501 ggatcagggg gaggcaggcg cgcgtgagtc ggaagacacc gaagacgttg acctcgaaga
     7561 gatcgcgcac cgcctcatcg gtgaaggagg ccaccggccc gatgccgccg aggccggcgt
     7621 tgttcacgac ggcgtagagg ccccggccgg ccgtgcgaat ggtttccgcg agggcaacta
     7681 cggatgcggg atcgcgcacg tccagtggca acgccatgac atccgggatt gcgtcgagac
     7741 cggccagatc atccggtttg cgggcggtgg cgtaaacggg caggccggcg gcggccagtc
     7801 gttcgacgat ggcgcgaccg ataccggtgc tggcaccggt gacgagaacg ggcttggaca
     7861 tgcacggcct ccttgtgggg ccgggatctt acgacagggg ggccccgtag tcgcgcagca
     7921 tggcggtgac ctgtgaaccc accttggtga agtgcacgcg caggtaggcc tcgaacggaa
     7981 aggggacgat gcgcggcgcg agccccagcg ccagccgcgc cagccacgcc cgcgcaatga
     8041 atcgcaggaa cgcggagttc gtgccgtgaa cgcgatccac gacacggagc gcgtgccgcg
     8101 cactcgccat cgccggcgca aggcgccggc catgaatgag gtcgcgcagc gaccaccccg
     8161 cctcaccgag cgccccgatc accggcagca tgagggcgga ggcctgcgcg ccgaggcccg
     8221 ccgcgccgcg tttgacctgt gccggcaggc caccgctccg cagcacattc acgatcgagc
     8281 cggcatccgg tccgtcgaag aaactggcgg aaaggggcgg cagccacaca ctcgtgccgg
     8341 gtccgcgcgc ctcgcccggc aacggcgagt gccatgccat gaagccgatg acgccatcca
     8401 cgtgccggtg cgatggaaac cattcccgaa gccgcgccgc gtcatgaagt cccggcgtca
     8461 ggccgacgat tgtcgtgctc ccgcttgccg cgatgagaga ctcgagccag ctccgatcga
     8521 gcgcatcgga gggcacgcac aaccagatct ggtcccagga gtgccccgcg acttcggcgg
     8581 gggtcgacac gacggtgatc ggccggaaaa cacccgtgtc ggtgcgatcg cgccccgtca
     8641 ctcgatacag cgtgaaccgc cgggcctcat ccacccgatg cggccggaca gataccccga
     8701 ccgacgcccc gcccaacgcc atgtaatgcc cgaagacctg cccaaccgct ccggcaccga
     8761 cgatcagcac gttcatccgg caggggtacg acggatcggc gtgccggtca agccggccgg
     8821 aggggaatcg ccggaacggc ggaaagggaa ggagtgagcc ggtagggagg gcgcattggg
     8881 acgaaattac acggtatcaa tgccccacgg agggctcaga accccctgca ggggcacgag
     8941 atgggtggcc cgccccttgc ttcgtatcag ggcattcccc atgatgaggc atctggccag
     9001 tgcgcacgga tgtccgggcc cgcaggcaac acgggtcctt tttgagccct tcctggaggc
     9061 atttgccatg aagaaagccg tctggatcag cctggctgcc gtcgttgtcc ttggaatgtc
     9121 cgcctgggtg tacaccctct cgaaggagaa gaagaccgtc gaggggcaat acgcacaggc
     9181 cgtggtcacc ggcgattcac ttcgtaccag cttcgacgtg gccctgtcgt cgatcgccga
     9241 aatccaggac agcctgttgg ccatcctgcc cagcgagtcg agcgtgatgc acatgtccga
     9301 ccaggtggaa accggcggac cgatgaccgc ttcgcgcaag gaccaggttt tgcgctccat
     9361 ctccgacctg cagggaagca tccagcgtag caaggagatg atcgcgggcc tcgagagccg
     9421 gctgggtgag caggacgtga agatcgggtc gctcgagaag atcatctccg gcctcaagcg
     9481 gacggtcagc gatcgcgagc acatgatcgc cgggctcaac aacaaggtcg agtcgctgac
     9541 ggtcgaagtc acgaacttga agaccgacgt ggccgcgggc caggcgcaga tcgccacgca
     9601 gcagcaggtg atcgaagaca agcgccgtga gatcagcacc gtgtactacg tcgtggacac
     9661 caaggacaac ctgaaggatg ccggcgtcgt gaagagcacc ggcggcgtca tcgggatcgg
     9721 caagtcgacc aagctgaccg gccagttccc gcagcaggtg ttcgcgcgtg tcgataccga
     9781 catgcagacc accattccgc tgtcgggcaa gaagccggtc gtcctttccg gccagagtgc
     9841 caccagctac gagatcgttc ccgtcaacga gcagatgtcg gagctccgca tcgtgaatcc
     9901 ggaggagttc cgcaaggtgc ggtacctggt cgtccaggtc ggctagacag tcttaccgga
     9961 ggcatccggt tcggccgacg cccttcggct gaaccaccgg ttcgcgagac cccggcccct
    10021 cgatggaggg gccggggtct ttcgttgttt ggtggtgtgg cggtcgctat catgttgcgc
    10081 atgacccgct cctccttcct cctgcgtttc ctcttcccag cgctggtgat tcccgcggtc
    10141 caggtgagtg cggccaatgc cctcggcccg tggagcccgg gagtcgtcga tcactggggc
    10201 tcggaaggga ccgggccggg gcaattcggc ctggcgcacg gcctggccgt tggaccggac
    10261 ggttccgtct acgtgggcga cacgctcaac aaccgcgtcc agaaattcac ggccgacggc
    10321 gactatgtcc tcgagttcgc ggtggctttc cccggtggcg tggacgtgga tgacgccggg
    10381 aacgtcttcg tcgtgggcgg cacggaccag gtcttcaagt tctcgtcgac cggcgagccg
    10441 atcacgtcgt ggggcggcac ggggagtgat cccggccagt tccgttttcc gctcgacgtg
    10501 gcggtcgatt cacaggggtt cgtctacgtc gtcgactgga tgaaccatcg ggtgcagaaa
    10561 ttcacgaacg acggcggctt cgtgctcgcg ttcggcagcg aggggacggg tgacgggcag
    10621 ttcatgacgc cggtgtcgat cgtatgcacc ggttccgacg tgattaccgt ccatgtcgtc
    10681 gacatggggc tggaccggac agtggccttc ctggggaacg gggagtggac cggcggcctg
    10741 cccggtgatt tgcacgaccc caccggcatc gccctcgagc acaccgggta cctcctgctg
    10801 ctcgatgccg gcagtggccg tatccatgtc tacggtccgg gcggcgaatt ccagttcgcc
    10861 ttcggcagcg aggggaacga gctcggacag ttcttccacc cctcggccat cgacgccggc
    10921 ccggacgggc ggatctacgt gatggacaag gacaaccacc gcgtgcaggt cttccagtcg
    10981 atcgcgtcgc ccatcgtgcc cgccacctgg agcgggatca agtcgctgtc ggctcgctga
    11041 ccgccaggaa ttcccgttcg agcgttccgg gggcgccgcg ccaggcgtcg ctcggcaggt
    11101 cccgtgcgat ctttccccgc acgatgatga gggcgcggtc gcagatcgtc tcggccgtct
    11161 cgaggacgtg tgtcgaaaag agcgcggtgc cgcctccggc ggcgaaatcg cgcaggacgc
    11221 cccgcatcac cacgcgcgat tcgggatcga ggccgttgag cgcctcatcg aggagcagga
    11281 gccgcgggcg gccgaggagc gcgccggcca tggaggtctt gcggcgcatg ccgtgcgaac
    11341 actcgaccag cagcgcgtcg gccttgtcgg tgaggcggaa ggcggccagc cagcggtcga
    11401 tgtcggccct gcgctcggcg atcggcagtt cgcggatggc ggccacgaac tcgaggaact
    11461 ggcgcaccgt gaggaagtcg tcgagcagct cggattccgg cacgtagccg atggcgcggc
    11521 gaagcgtgat gtcgccgaag gggacgggtt ttccatccac ctggagagat ccggcgtcgg
    11581 ggggcaacag gcccacaaga attcggatga gcgtgctctt gccggcgccg ttccgcccca
    11641 ggaggccgca gatctggccg ggcgcgagcg tgaacgacac gtccgagagc gcccggacgg
    11701 cgccataact cttcgacagg cccgtaatcg tgatcatgcc gcccacgcct cgaaggccgc
    11761 cggcgccttg cgcgcccaga ctcccagcac cagggccgcc accgcaaaag tcgcgatcgg
    11821 gaccagtacc cacgtgacca ggaagagcac cgcggcgatg ttcaggaaaa agccggccag
    11881 cgtaaggcgc gggaagagcg ccac
//
LOCUS       DASVOD010000776         5211 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_728647, whole genome shotgun sequence.
ACCESSION   DASVOD010000776 DASVOD010000000
VERSION     DASVOD010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728647"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2280
                     /locus_tag="VF720_16605"
     CDS             133..2280
                     /locus_tag="VF720_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881033.1"
                     /translation="MSNGRMRRTFSAALGVLGVLLVVVAVMLGFARRVLFDERAFSAR
                     VAASLEDSRVAGWVAERLADGVIAVQPDLVGVRPLLVGVGRGIVISPPFRAAARRTAR
                     TAHRALMSGTGQDILLTVKDVGVVLSSAVSEKPAIAGKIPPKLAASIGALKSLPGGER
                     AMRLVQFAHRLRAGAIALFLAGIGLCVLSVRLAEGRRVAIVRIGTAFAVAGLVMVIGA
                     RFGGDLLSLFARDESAAPALAGLGRAFLRPLVVWGFGLGFSGLVFASAAASLLERVSM
                     EQWARDTQGWLFGAQHRMWLRMVRGLVGLAIGAAILSAPLASLTVFAWLAGVVIAFAG
                     LREAFHALLHTLPEVDRTAARRAPGTPGRLAVGVIGLVAVAVIATTAWLVLRTSHREA
                     VAGDLLAVNGLPELRDRTIDQVVFPGTHNAMGSPDIPGWMFPNQGADIRAQLGSGIRA
                     FMLDALYAAPVGDRVKTEMKEGAPGLAKYQAAIGAEGMDAAIRIRERLVGSEEGERDV
                     YMCHGFCELGARKLADGLEDMHDFLVENPDEVLILIIQDVDVAPADIARCFEESGLID
                     FVYRGPLGPPWPTLRSMVESNQQVIVFAENDWAGIDWYHPAFETFQETPYTFHEPDQF
                     SNKPNRGGTGGSLYLMNHWIETTPMPKPTNAEIVNAHDFLLARIEGFEKERRHLPNIV
                     AVDFFQSGDLVAVTRELNLRPVDEPLPKKGRRR"
     gene            complement(2287..3342)
                     /locus_tag="VF720_16610"
     CDS             complement(2287..3342)
                     /locus_tag="VF720_16610"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX7881034.1"
                     /translation="MTIEKTEGERPQDQVALFIDFENIRYSVLNTYGREVGGQMLMEK
                     ARKHGLVTLSRAYADFSEHPDRVQRDLQVSGITAINVAAHKMGDSKKSGADMEMLMDV
                     FETFLDRPFVKTFVLMTGDRHFIRAVTIARNRYGKQVIIAGVPGSVSNDLVEAAGSNF
                     DPLVFEPVGATERFTSFVRFCDRLERSKPFITFKYISAAMSTSSEFPGLQEEEARQLV
                     ADAIRDGILLKEQRTDGYRICKLNRSHGGVMDVIAGRPVTNLPEHHAAAAAAIQALAP
                     PMPMSTNGHGGHAHAGNGVTQGYRPATSAFGPTAIDPQEEWRRRARELGLPDVEEEEE
                     AMYRTSSPMPAPELPTE"
     gene            complement(3588..>5211)
                     /locus_tag="VF720_16615"
     CDS             complement(3588..>5211)
                     /locus_tag="VF720_16615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881035.1"
                     /translation="IREVLYAEANPIRRRRLHARILEAIERIHADRLDAHATDLAHHA
                     VQAGAMEKGFDYSLRAVNRSESVYAHDEARRYADLARECAEALGDRPRIVEAWRARSR
                     VESVSGASAAAAAALVEALPWVDDADQRNAIEVNIGLHYVSVGDHRGLPHLESVMQRL
                     DTAADPDLAARALSIRARYHHYDLEHERALELLVEARRLAAVKDNTGTLTSVMSYIAG
                     AHQHMFQVDRSLAMGTELMEMGRARGNRFATALGHEFIGEAKALAGLWRESVAASEEV
                     RRLGIEMGARDRIAWADFIAMYARFQGGRLRRSLADGRHGLELAVQIGELRLAALIAG
                     YLTHILAELGERETWRAMADEATRLVEAAGQRWMHLLSRYCLAGAHLAEGDHVTATKI
                     LADPGLRQLGDNRAALIVGLGIRVECELAAGRFESALGVAEQLATLGVETKASFQEGI
                     ARRVAAQACVALGRWEEAMVEIRRAIALQESHDHQFELAKSRAALADMLDARRASGKF
                     SPFENETLAEEAAAERVESVRLQHASRSGEMG"
BASE COUNT          740 a         1755 c         1834 g          882 t
ORIGIN      
        1 ggcgcatgcg tgctccgtcg gtggatttcg gtccgcgcgc atcatggcag acccgggcat
       61 tcccggatag gccggcgggg gatgtcccga tgcccggctt gttggtagtg tccgccttca
      121 cgggagggaa ccatgtccaa cggccgcatg cgccgcacgt tctcggcggc actcggagtt
      181 ctcggcgtcc tgctcgtcgt cgtggccgtc atgctcggct tcgcccggcg ggtgctgttc
      241 gatgagcggg ccttttccgc gcgcgtggcg gccagcctgg aagattcccg ggtggccggc
      301 tgggtggccg aacggcttgc cgacggcgtc atcgcggtac agcccgacct ggtcggcgtg
      361 cgcccgcttc tcgtgggggt ggggcgcggg atcgtgatct cgccgccgtt ccgggccgcg
      421 gcccggcgca ccgcccgcac cgcccaccgg gccctgatga gcggcaccgg tcaagacatc
      481 ctgctcacgg tgaaggacgt cggcgtggtg ctgagcagcg ccgtgtcgga gaagccggcc
      541 atcgccggca agattccccc gaagctggcc gcgtcgatcg gggcgctcaa gtcgctcccg
      601 ggaggcgagc gggccatgcg gctggtgcag ttcgcccacc gattgcgggc gggcgcgatc
      661 gccctgttcc tggccggcat cggcctgtgt gtcctcagcg tccgcctggc cgaggggcgg
      721 cgggtggcca tcgtccgcat cggcaccgcg ttcgcggtgg ccgggctcgt gatggtgatc
      781 ggcgcccgct tcggcggcga cctgctctcg ctcttcgccc gcgacgaatc ggcggcgccg
      841 gcactggccg gtctgggccg ggccttcctc cggccgctgg tggtgtgggg gttcggcctc
      901 ggcttctcgg ggctcgtgtt cgcctcggcc gcggcctcgc tgctcgagcg tgtgtcgatg
      961 gagcaatggg cccgggacac gcaggggtgg ctgttcgggg cccagcaccg gatgtggctc
     1021 cggatggtgc gggggctcgt gggcctcgcc atcggcgcgg ccatcctgtc ggcgccgctg
     1081 gcttcgctca cggtgttcgc ctggctggcc ggcgtcgtca tcgccttcgc cgggttgcgc
     1141 gaagcgttcc atgcgctgct gcatacgttg ccggaggtcg atcggacggc tgcccgacgg
     1201 gcgcccggca cgccgggccg gctggcggtg ggtgtgatcg gtctcgtcgc cgtggcggtg
     1261 atcgccacga ccgcctggct ggtgctccgc acgagccatc gcgaggccgt tgccggcgac
     1321 ctgctggcgg tcaacggcct gccggaactg cgcgacagga ccatcgacca ggtggtgttc
     1381 cccggcacgc acaacgcgat ggggagcccc gacattcccg ggtggatgtt ccccaaccag
     1441 ggcgccgaca tccgcgcgca gctcggctcc ggcatccggg ccttcatgct cgacgcgctg
     1501 tacgcggcgc cggtcggcga ccgcgtgaag accgagatga aggagggggc gcccggcctg
     1561 gccaagtacc aggccgccat cggcgcggag gggatggacg cggccatccg catccgcgag
     1621 cgcctcgtcg gatcggagga gggagagcgg gacgtgtaca tgtgccacgg cttctgcgag
     1681 ctgggtgccc gcaagcttgc cgacgggctc gaggacatgc acgacttcct ggtcgagaac
     1741 cccgacgagg tgctgatcct catcatccag gacgtggacg tggcgccggc cgatatcgcc
     1801 cgctgcttcg aggagagcgg cctgatcgac ttcgtctacc ggggaccgct cggcccgccg
     1861 tggcccacgc tgcggagcat ggtggagagc aaccagcagg tgatcgtctt cgcggagaac
     1921 gactgggccg gcatcgactg gtatcacccg gccttcgaga cgttccagga gacgccgtac
     1981 accttccacg aaccggacca gttctcgaac aagcccaacc gaggcggcac cggcggttcg
     2041 ctctacctga tgaaccactg gatcgagacg acaccgatgc ccaagccgac gaacgccgag
     2101 atcgtcaacg cccacgactt cctgctcgca cggatcgagg gattcgaaaa ggagcgccgc
     2161 cacctgccga acatcgtggc ggtcgatttt ttccagtcgg gcgacctggt ggccgtgacc
     2221 cgggagctca acctgcggcc ggtcgatgag cctctgccga agaaggggcg acgccgctag
     2281 ccggccttat tccgtcggca attccggggc cggcatcggc gaggaggtgc ggtacatcgc
     2341 ttcctcttcc tcttccacgt ccggcaggcc cagctcgcgc gcccggcgcc gccattcttc
     2401 ctgcgggtcg atggctgtgg ggccgaaggc cgaggtggcc gggcggtaac cctgcgtgac
     2461 gccgttgccg gcatgcgcat gcccgccgtg accgttcgtg ctcatcggca tcgggggcgc
     2521 cagggcctgg atggccgcgg ccgcggccgc gtggtgttcg ggaaggttcg tgaccgggcg
     2581 gccggcaatg acatccatca cgccgccgtg gctccggttg agcttgcaga tccggtagcc
     2641 gtcggtgcgc tgctccttga gaaggatgcc gtcgcggatg gcgtcggcca ccagctgccg
     2701 ggcctcttct tcctgcaggc cggggaactc gctcgaggtg gacatggccg ccgagatgta
     2761 cttgaaggtg atgaacggct tcgagcgctc gagccggtcg cagaagcgga cgaacgaggt
     2821 gaagcgctcg gtggcgccca ccggttcaaa gacgagcggg tcgaagttgg atccggccgc
     2881 ctccaccagg tcgttggaaa ccgacccggg gacgccggcg atgatcacct gcttgccgta
     2941 acgattccgg gcgatcgtga ccgcgcgtat gaagtggcgg tctccggtca tgaggacgaa
     3001 tgtcttgacg aaggggcggt cgaggaaggt ctcgaacacg tccatgagca tttccatgtc
     3061 ggcgcccgat ttcttgctgt cacccatctt gtgggccgcc acgttgatgg ccgtgatccc
     3121 ggaaacctgg aggtcgcgct ggacgcggtc cgggtgctcg ctgaaatcgg cataggcgcg
     3181 ggagagtgtg acgaggccgt gcttgcgggc cttctccatc agcatctgcc cgcccacttc
     3241 gcgtccgtac gtgttcagga cactgtaacg aatattctcg aaatcgatga agagcgcgac
     3301 ctggtcctgt gggcgctcgc cttcggtctt ttcaatggtc atgggtgtga aaatcctctc
     3361 gtcgggggtc atgacccgcg ccgggggcgc acgggggcac acgaagccca tgggcgcgcg
     3421 gtacgcgggg gggcggccgg agaaccggcc gaaacattga gatggctgtt tgcgatggtc
     3481 aggaacacaa tcgattcggt ttccagaagc aaccaacgga ctgactcaat gaactgatgg
     3541 gaccgattct aaccgcgtgc cccgccgacg tccagaggtt ttccatccta ccccatttcg
     3601 cccgatcgcg atgcgtgttg cagtcggaca ctttcgaccc gctccgccgc cgcctcctcc
     3661 gcgagtgttt cgttctcaaa cggtgagaat tttcccgatg cccgccgcgc gtccagcata
     3721 tcggccagcg cagcccgcga tttcgcgagt tcgaactggt gatcgtgtga ctcctggagc
     3781 gcgatggccc gccggatctc gaccatcgct tcctcccacc ggccgagcgc gacgcaggcc
     3841 tgcgccgcca cgcgacgcgc gattccttcc tggaaggacg ctttcgtctc gactccgagc
     3901 gtggccagtt gttccgccac cccgagcgcc gactcgaatc gtcccgcagc gagctcgcat
     3961 tccacccgga tgccgagccc cacgatgagc gcggcccggt tgtcgcccag ctgccggagc
     4021 ccggggtcgg ccaggatctt ggtggcggtc acgtggtctc cctcggccag gtgagcgccc
     4081 gccaggcagt agcggctcag gaggtgcatc cagcgttgcc cggcggcctc gacgagtcgt
     4141 gtggcctcgt cggccatcgc ccgccacgtt tcccgctcgc cgagctcggc caggatgtgc
     4201 gtgaggtaac cggcgatgag cgcggcgagc cggagctcgc cgatctggac agcgagctcg
     4261 aggccgtgcc gaccgtcggc cagtgagcgt cgcagccgcc caccctggaa gcgggcgtac
     4321 atggcgatga agtcggccca ggcgatccga tcccgcgccc ccatctcgat gccgagccgg
     4381 cgcacctcct cgctggcggc caccgattca cgccagaggc cggcaagagc cttggcctcg
     4441 ccgatgaact cgtggcccag tgcggtggcg aaacggttgc cgcgcgccct ccccatttcc
     4501 atgagctcgg tccccatggc caggctcctg tcgacctgga acatgtgctg gtgcgcgccg
     4561 gcgatgtacg acatgacgct cgtgagcgtg ccggtgttgt ccttcaccgc agccagccgg
     4621 cgcgcttcca cgagcagctc gagtgcccgc tcatgctcca ggtcgtaatg gtggtagcgg
     4681 gcgcggatcg agagcgcccg ggccgccagg tcgggatcgg ccgccgtatc gaggcgctgc
     4741 atcaccgact cgaggtgggg aaggccgcga tggtcgccga ccgacacgta gtgcaggccg
     4801 atattgactt cgatggcatt ccgctggtcg gcatcgtcca cccacggaag cgcctcgacc
     4861 agcgccgcgg ccgccgccgc cgatgcgccg gacaccgatt ccacgcgcga gcgagcccgc
     4921 cacgcctcca cgatccgcgg gcgatcgcca agcgcctcgg cgcattcccg cgccaggtcg
     4981 gcatagcggc gtgcctcgtc gtgggcatac accgactcgg aacggttcac ggcccggagc
     5041 gaatagtcga accctttctc catggcgccg gcctggaccg cgtggtgcgc gaggtcggtg
     5101 gcatgggcat cgagccggtc ggcatggatc cgctcgatgg cctcgaggat ccgggcgtgg
     5161 aggcgtcgcc ggcggatcgg gttggcctcg gcgtagagga cctcgcggat c
//
LOCUS       DASVOD010000777         7191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_573591, whole genome shotgun sequence.
ACCESSION   DASVOD010000777 DASVOD010000000
VERSION     DASVOD010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573591"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2948)
                     /locus_tag="VF720_16620"
     CDS             complement(<1..2948)
                     /locus_tag="VF720_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881036.1"
                     /translation="MTPLAARRNPALIVAGLMILVTQVDANTYPVAYSDNQPRATATY
                     VEDHHDHGAPHEWHSGVDIPRTAGGTLVRTAQAGTLGTVGNGSGMSGAVYVAAGQATA
                     DGPWSILLPPGPPLYLLDVISVTRTIPGIGTTTYTVESVGSLLPGFGSGRINLAANQP
                     DPGAALPVGSQVDVSYFYYSAVVRSMTFDVAGDYHQYAHIANVHQYFDWDMYPATAAN
                     RVYGYADVPPGRSPLVLPEGFTFAAIDTLLPFDNELPHVHFSQFVGGDAFPANTVNPL
                     YYGTLGTDDPMNMRPEVEEIEFRRNGVNQLVPAAADRTIVYGRLDIFADCRDSMGGVE
                     VGGTDTDADTDRETGAFNAEYWITANGSGGSNVASSAAPNAMFKAPGVTELPILPLAD
                     VFWLDSQFELDVNTDYHLCVTHVNEDRLRHWASDAPSTATNSDGSDAVDAARANERAK
                     FKDGPYDVHARAADVVGTGNERTEQVVVDNFRPMVKEVFVEDANGPLYHATWNLTTGD
                     EQYELDPPDPDEAMLDEFGNKQAASGQFAVDIRVTFTEAMQNAEISIQPLGLDLLLYQ
                     DGNDATVWSTTIAPQDLSNHPDKSGKQMLTITGIDWAGNELLQLSESVPVPTADLRRD
                     AAGQFQPLGGADTIHRFGICTAVTFIIDDTGSMTEELEGIKETCVQMINEDTDNPDFF
                     PLYRLYTFKDNVTFREQSEDPGEVIPEIQQLEADAGGLCPENSPDALYQAAQNMQSGG
                     VAYFATDASANQFSRTLESAMSAMENNDVQVNELLTDVLCQDNPSGVPNGLDPEAWGC
                     PDQDCGGTSGSGDTELQAIVPDGVMYEYRRMAAETGGILIHATKPEAGLALKAIHANR
                     ASNALLFSVRDTLTLPGMLSYPVPVDSSVSKLFILFDPVAAESLVMVVHRPGGAVVDT
                     LDADVVPLVGTALTFLTIDAPIAGEWLFEISGRGSFQLRVGAASPFTFRVVGAPRLAP
                     GRATPVELTFP"
     gene            complement(2945..3892)
                     /locus_tag="VF720_16625"
     CDS             complement(2945..3892)
                     /locus_tag="VF720_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881037.1"
                     /translation="MGNETESRKTRTHLTILMILGCFLTRTLTAVPASAREPGALNPT
                     MARDVDVIDWYASEARALPPEVLPVDLDRAIVALRRGQAYTHTFTSPSGARRITVVYR
                     LDVGSRVPHVMATATVDESGVTFDVLPFLLFDLADGGTAIVGHGSLFEEAASEGPLRY
                     QLAFYEPDGRQYRPELEIGLGTWRSSCFLDGGGTFVVGLPGTIVAFDTGKGTLRWRHD
                     LQDGGWPKVSANPAGSRLAALIRKPADAWTIELLDSGGTALGKSALHGRPAALRALFD
                     QSGEHVFIPCSRSDSGETATTLELRSASDLSVQRIVRLP"
     gene            complement(3876..4556)
                     /locus_tag="VF720_16630"
     CDS             complement(3876..4556)
                     /locus_tag="VF720_16630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX7881038.1"
                     /translation="MRKVLQTARDGDPKAPAGPVAETGRVRQKSRTRRHITDVARRLG
                     AQGKLQSLADLADEAEVSRATVYRYFSSLADLDAEMALAVQVKEPDELFRGEEAGALP
                     RVLKVHAHLFGLMSQHEAGFRAYLKRSMEQWDRSGRKPGTLREGRRARLLDAALVDLK
                     RQLNRNDYQKLVQVLSLLVFIEPYILLKDIYGMTDAQADRTMRWGIERLLSGTLHMAR
                     GDRHGKRD"
     gene            complement(4675..6348)
                     /locus_tag="VF720_16635"
     CDS             complement(4675..6348)
                     /locus_tag="VF720_16635"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7881039.1"
                     /translation="MARRSPLLTALALLVLPASALGAQPYYEDWDAFDDVAGWVMNTI
                     AVTISHSNVGGNPNGYLVSTREGSGVFSAGATSELPAVSGDYAAAGITEVSFDVLYSP
                     EQPGANLQGGGPAYFAAAFRVRYQGPANNGWTFGLPNDFTPGWKSYTITFDPTWTDME
                     AVAAGWVPDDPMGDSFATTMSDVYHPEVRIDGNGTLVVGIDNFRLSAGACDEDVTGPT
                     VDVTLSRDTLWPPNHKMIEVCAEVTAEDNCDSDVDVDLVSVTSSEPENGLGDGDTEGD
                     IEILENGCFLLRSERAGGGDGRKYSIVYSATDNAGNTTYDTTCVMVPHSRNGHAHVAS
                     GIAGGGFQAGASQYALTVLSRPGFDARQLDPSRAFVGNDVGYVRPVESGITDVNGDHL
                     TDRVFLFDTSDTQDLIDASVARVEAEAGATYQVGDGELELARPRPTPAAFYYEGPDGA
                     SFLETNVLRIPGVQQAPTPKFEEAPDFELAGNVVRFAVPEPSRISITLFNVLGQKVRT
                     LVAEDLSAGVHEVAWDGTDDSGSLAPAGIYFARMSRPTGVSTARMLLSR"
     gene            complement(6455..>7191)
                     /locus_tag="VF720_16640"
     CDS             complement(6455..>7191)
                     /locus_tag="VF720_16640"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX7881040.1"
                     /translation="RTGYLEMPTWALYNSQWDWRSFLDETFATLAGNGATDLVIDLRL
                     NEGGSNVGDVIVSHLSGSPVTRPAIARLVRYRKVPDDLAPYLDTWDPGFKDWGDAAID
                     SSGRFFRLRRDADDDRNAAIMPVPPRFAGRVWVLVGPTNSSATFEFAQTVRENRLGTL
                     VGQSTGGNQRGINGGAFFFLRLPHSGIELDLPLIGQFRGGEWPDAGLDPDVLVEPTVD
                     DIARGRDAELEAVRALIGPTDGVPGR"
BASE COUNT         1205 a         2361 c         2372 g         1253 t
ORIGIN      
        1 gggaacgtga gctcgacggg cgttgccctt ccgggcgcca gccgtggcgc gccgacgacc
       61 cggaacgtga agggcgacgc ggcgccgacc cgcagctgga acgagccgcg tccgctgatt
      121 tcaaacagcc actccccggc aatcggtgcg tcgatggtga ggaaggtgag cgcggttcca
      181 accagcggaa ccacgtcggc gtccaacgta tccactacgg cgccgcctgg tcggtgcacc
      241 accatgacga ggctttccgc cgccaccggg tcgaaaagga tgaagagctt cgacacgctc
      301 gaatcgaccg gcacggggta actcagcatc cccggaaggg tcagcgtgtc ccggaccgag
      361 aagaggagcg cgttgctggc acgattcgcg tggatggcct tcaacgccag gccggcttcg
      421 ggcttggtgg catggatgag gatgccgccg gtttccgccg ccatccgccg atactcgtac
      481 atgacaccgt caggaacgat cgcctggagt tcggtgtcgc cgctccccga agtgcctccg
      541 caatcctggt ccgggcaacc ccatgcttcg ggatccaacc cattggggac gccactcggg
      601 ttgtcctggc acagcacgtc ggtcaggagt tcgttgacct ggacgtcgtt gttttccatg
      661 gccgacatcg ccgactccag cgtccggctg aactggttgg ccgaggcgtc cgtggcaaag
      721 taggccacgc ctcccgactg catgttctgc gcggcctggt agagcgcgtc cggtgaattc
      781 tccgggcaga ggcccccggc gtccgcctcg agctgctgga tttccgggat gacttcgccg
      841 ggatcctccg actgctcgcg gaacgtgacg ttgtccttga acgtatacag ccggtagagc
      901 ggaaagaagt ccggattgtc cgtgtcttcg ttgatcatct ggacgcaggt ttccttgatc
      961 ccctcgagct cctcggtcat gctgccggta tcgtcgatga tgaaggtcac ggccgtgcag
     1021 atgccgaagc ggtggattgt gtccgcaccg cccagcggct ggaactggcc ggccgcatcc
     1081 cgcctgaggt ccgccgtggg caccggaacg ctctccgaga gctggagcaa ctcgttcccc
     1141 gcccagtcga tcccggtgat ggtcagcatc tgcttcccgg acttgtccgg gtggttcgac
     1201 aggtcctgtg gcgcaatggt ggtggaccag accgtcgcgt cgtttccatc ctggtaaagg
     1261 aggaggtcga gcccgagcgg ctgtatcgag atctccgcat tctgcatcgc ctcggtgaac
     1321 gtcacccgga tatccaccgc gaactggccg ctggccgcct gcttgttgcc gaattcgtcg
     1381 agcatcgcct cgtccggatc cggcgggtcg agctcgtact gctcatcccc ggtcgtgagg
     1441 ttccatgtgg cgtgatagag ggggccattg gcgtcctcga cgaagacttc cttcaccatc
     1501 ggccggaagt tgtcgacgac gacctgttcg gtacgctcgt tcccggtgcc caccacgtcc
     1561 gcggcccggg catggacgtc gtacggacca tccttgaact tggcccgctc gttggccctg
     1621 gcggcatcca cggcatcgga gccgtcgctg ttcgtggcgg tggagggagc atcgctggcc
     1681 cagtgccgca gccggtcctc gttcacatgg gtgacgcaca gatggtagtc ggtgttgacg
     1741 tcgagctcga actggctgtc cagccagaaa acgtcggcca gcggaagtat gggcagctcc
     1801 gtcacgcccg gggccttgaa catcgcgttc ggtgcggccg atgaagccac gttggaaccg
     1861 ccgctgccgt tggcggtgat ccagtactcg gcattgaacg caccggtctc ccggtccgtg
     1921 tcggcgtcgg tgtccgttcc gcccacttcc acgcccccca tgctgtcgcg gcaatcggca
     1981 aagatgtcga gccgtccgta cacgatggtc cggtcggcgg ccgccgggac cagctggttg
     2041 acgccgttgc gccggaactc gatctcctcg acctccggac gcatgttcat cgggtcgtcc
     2101 gtgccgagtg ttccgtagta gagggggttg accgtgttgg ccgggaaagc gtcacctccg
     2161 acaaactggc tgaagtgcac atgcggcagc tcgttgtcga acggcaggag cgtatcgatc
     2221 gccgcgaagg tgaacccttc cgggagcacc agcggagagc gtcccggcgg cacgtcggca
     2281 tacccgtaga cgcggttcgc tgccgtggcg ggatacatgt cccagtcgaa gtactgatgc
     2341 acgttcgcga tgtgggcata ctggtggtaa tcgccggcaa cgtcgaacgt catgcttcgc
     2401 acgacggcgc tgtaatagaa gtagctgacg tcgacctggg agcccacggg cagggccgcc
     2461 ccgggatcgg gctggtttgc tgccaggttg atgcgtccgg agccgaagcc gggcagaaga
     2521 gagccgaccg actcgacggt atacgtcgtg gttccgatcc ccggaatcgt acgcgtgacg
     2581 gagatgacgt ccagaaggta caacggcgga cctggaggca ggaggatgga ccagggtccg
     2641 tcggccgtgg cctgccccgc cgcaacgtac acggcgcccg acatcccgct cccgttcccg
     2701 accgtaccga gcgtcccggc ctgcgcggtt cgcaccaggg tgccgcccgc cgtccgcgga
     2761 atgtccacac ccgagtgcca ctcgtgtggg gcaccatggt cgtgatggtc ctccacgtag
     2821 gtcgcagtgg cgcgcggctg gttgtcggag taggccaccg gataggtgtt cgcatcaacc
     2881 tgcgtcacca ggatcatcaa tccggccacg atcagggccg gattccggcg ggcggccaac
     2941 ggggtcatgg cagcctcacg atccgctgga cgctcaagtc ggatgccgag cgcagctcga
     3001 gtgtggttgc cgtttcgccg gaatcggaac gggagcaggg tatgaagacg tgctccccgc
     3061 tctggtcgaa cagcgcccga agcgccgccg gccgtccgtg tagcgccgac ttacccagcg
     3121 cggtgccgcc gctgtcgagc aattcgatcg tccaggcatc ggccggtttt cggatcagcg
     3181 cggccagtcg ggagcccgcc gggttggccg atactttggg ccagccgccg tcctgcagat
     3241 cgtgccgcca tcgcaacgtc cccttcccgg tatcgaaggc cacgatcgtg ccgggcaggc
     3301 ccacgacaaa cgttcctccc ccatcgagga agcaggagga tcgccacgtc ccgaggccaa
     3361 tctcgagctc aggccggtac tggcggccgt cgggttcgta gaaggccagc tgataacgga
     3421 ggggcccctc gctggcggcc tcctcgaaga gcgaaccgtg gcccacgatg gccgtgccgc
     3481 cgtccgccag gtcgaagagc agaaacggca gcacgtcgaa ggtgaccccg ctctcgtcca
     3541 ccgtggccgt ggccatgacg tgcggtacac ggctacccac gtcgagccga tagacgaccg
     3601 ttatccgcct cgcgccggac ggcgaggtaa atgtgtgcgt gtaggcctgc ccccgccgca
     3661 gcgcaacgat ggcacggtcg aggtcgacgg gcaggacttc cggaggaagg gcgcgggctt
     3721 cggaggcata ccagtcgatg acgtcgacat cgcgggccat ggtcggattc agcgcgcccg
     3781 gctcgcgagc ggacgccgga acggccgtga gcgtccgtgt caggaaacag cccaggatca
     3841 tgaggatggt gaggtgggtg cgggtcttcc gggattcagt ctcgtttccc atgtcgatct
     3901 ccccttgcca tgtgcaacgt cccggaaagg aggcgttcaa tgccccagcg catcgttcga
     3961 tccgcctggg cgtcggtcat cccgtaaatg tccttgagca ggatgtaggg ctcgatgaag
     4021 accaggaggc tgagcacctg caccagcttc tggtagtcgt tccgattcaa ttgccgcttc
     4081 aggtcgacca gggccgcgtc cagaagccgg gcccgccgcc cttcgcgaag cgttcccggc
     4141 tttcggcccg accggtccca ctgctccatc gagcgcttga ggtacgcccg aaagcccgct
     4201 tcgtgctggc tcatgagccc gaagaggtgc gcgtgcacct tgaggacccg cggcagtgcc
     4261 ccggcttcct cgccacggaa cagttcgtcg ggctccttga cctggacggc cagggccatc
     4321 tccgcgtcga gatccgccag gctcgagaaa tatcgataga ccgtggcccg cgacacctcc
     4381 gcctcgtccg ccaggtccgc caatgactga agctttccct gcgcccccag tcgtcgcgcc
     4441 acatcggtga tgtgccgccg tgtccggctc ttctgccgga cccgccccgt ttcggcgaca
     4501 ggacccgccg gagccttcgg atctccgtcg cgagctgttt gtaagacttt tctcatgagt
     4561 agaagttatc tcatgaccat gggatcgtca agcaattccc cgaaaacgaa acggcacccc
     4621 cccggccagg ccaggggggt gccgtcaggt gatcgacgtg acgacttcag gacatcaacg
     4681 gctcagcagc atccgggccg tcgaaacgcc cgtcggccgg ctcatccgag cgaagtagat
     4741 ccccgcggga gcgagcgaac ccgagtcgtc cgtgccatcc cacgccacct cgtgcacgcc
     4801 ggccgagagg tcttcggcca cgagcgtccg gaccttctgc cccaggacgt tgaagagggt
     4861 gatcgaaatg cgggacggct ccggcacggc aaagcgcacc acgttgccgg ccagttcgaa
     4921 atcgggcgct tcttcgaact tgggcgtcgg cgcctgctgg acgcccggaa ttcggaggac
     4981 gttggtctcc aggaacgagg cgccatcggg gccttcgtaa tagaaggcgg ccggggtcgg
     5041 acgcgggcga gcgagttcga gttcgccgtc gccgacctgg taggtggcgc cggcttccgc
     5101 ctcgacacgg gccacggagg catcgatcag gtcctgcgtg tccgaggtgt cgaacaggaa
     5161 gacgcggtcg gtgaggtggt caccgttcac gtcggtgatg ccgctttcga ccgggcgcac
     5221 atagcccacg tcgtttccga cgaacgcgcg cgagggatcc agctggcggg catcgaaccc
     5281 cggccgcgac agcaccgtca gggcgtactg cgaggctccg gcctggaagc ctccgccggc
     5341 aatcccggag gccacgtgcg catgaccgtt ccggctgtgc ggcaccatca cgcaggtggt
     5401 gtcgtaggtc gtgttccccg cgttgtcggt ggccgaatag acgatgctgt acttgcggcc
     5461 atctccgccg ccggcgcgct cggaccggag gaggaagcag ccgttttcga ggatctcgat
     5521 gtcgccttcg gtgtcgccgt cgccgaggcc gttttccggc tcgctgctgg tcaccgagac
     5581 caggtcgacg tcgacgtccg aatcacagtt gtcctcggcg gtaacctccg cgcatacctc
     5641 gatcatcttg tggttcggcg gccagagggt gtcgcgcgac agggtcacgt ccaccgtcgg
     5701 gcccgtcaca tcctcgtcgc aggcgccggc cgagagccgg aaattgtcga tgccgacgac
     5761 cagcgtgccg ttgccgtcga tccgcacttc cgggtgatac acgtcggaca tcgtggtggc
     5821 aaaactgtct cccatcggat catccggaac ccatccggcg gcgacggctt ccatgtcggt
     5881 ccacgtggga tcgaaggtga tcgtgtacga cttccagccc ggcgtgaagt cattcgggag
     5941 gccgaacgtc cagccgttgt tggccgggcc ctggtaccgc acgcggaacg ccgcggcaaa
     6001 ataggccggt ccgcccccct gcaggttggc ccccggctgc tccggggagt agagcacgtc
     6061 gaacgacacc tcggtgattc ccgccgcggc atagtcaccg gacaccgccg gcaattcgct
     6121 cgtggcgccg gcggagaaga ccccgcttcc ttcgcgtgtc gacaccaggt atccattcgg
     6181 attgccaccc acattggagt gactgatcgt gacggcaatc gtgttcatca cccaccccgc
     6241 cacgtcatcg aacgcatccc agtcttcgta atagggttgc gcccccagtg ccgatgcggg
     6301 gaggaccagg agagcgagag cggtaagcag gggactgcgg cgggccatgg gggcctcctt
     6361 gattggggtt gtgccgacgc tgtcggaaac aggagaaccg tggtagggac aggaagggaa
     6421 agctaggact gcgggaacga caagtcaaca ataatcagcg ccccggaacc ccatccgtcg
     6481 gccctatcag cgcccgcacc gcctcgagtt ccgcgtcccg gccccgggcg atgtcgtcca
     6541 ccgtcggctc cacgaggacg tcgggatcga ggcccgcgtc gggccattcc cccccccgga
     6601 actggccgat caggggaagg tcgagttcga tgccggaatg gggcaggcgc aggaagaaga
     6661 aggcgccgcc gttgatgccg cgctggttgc cgccggtgga ctggccgacg agcgtcccga
     6721 gccgattctc acggaccgtc tgcgcgaact cgaacgtggc ggaactgttc gtcgggccaa
     6781 ccagcaccca cacgcgtccg gcaaatctcg gaggcacggg catgatggcc gcgttgcggt
     6841 cgtcgtcggc atcccgtcgc aaccggaaga agcgcccgct cgaatcgatg gccgcatcgc
     6901 cccagtcctt gaatcccgga tcccacgtgt cgagataagg cgccaggtcg tccggaacct
     6961 tgcgataacg cacgagcctc gcgatggccg gacgcgtcac cgggcttcca ctgaggtggc
     7021 tcacgatcac gtcgccgacg ttgctccctc cttcgttgag cctgaggtcg atgaccaggt
     7081 cggtggcgcc gttgccggcc agcgtggcaa atgtctcgtc caggaaactc cgccagtccc
     7141 actggctgtt gtacagcgcc caggtcggca tctcgaggta acccgtccgt g
//
LOCUS       DASVOD010000778         8685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_624996, whole genome shotgun sequence.
ACCESSION   DASVOD010000778 DASVOD010000000
VERSION     DASVOD010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 8685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8685
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624996"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF720_16645"
     CDS             complement(<1..183)
                     /locus_tag="VF720_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881041.1"
                     /translation="MRRVFGSRAFWVPAAVLTLSLGQSLPARASFEALEVDPRARALG
                     GAATGVDLGWLSAWHNP"
     gene            complement(219..3941)
                     /locus_tag="VF720_16650"
     CDS             complement(219..3941)
                     /locus_tag="VF720_16650"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX7881042.1"
                     /translation="MRFYRPRRAAPLGLLALLPIAVLALSALPSPVSAQTPAKLLLQS
                     VCILGTDQEYVAVYNPTNASVDLSNYYLTDALFTNQGYWFLPDPTKRNSVSSGGGTFN
                     DWHVRFPAGSMIAAGDTVAVSIQGSAAFVRAYSRKPAFEILAAGLTDDASVPNMREAF
                     PGSVPASANGATAHGLTNSGESVTLYYWDQQSDLVIDVDYLVWGNDTGMFRYFNIKTG
                     VTVDGPDGDSNASAYAADTPVAQQSFIVPAEAAQAHTFGNAFRREDYNEGTQATSGGN
                     GIGGRNETSENLAVTWSKDQPAMPPFAKGGGPVIVDLVAKPGGPYTGIVNQAVSFNGS
                     ASTPATGETITGYNWNFGDGMTGTGATPTHTYTTAGTFAVMLTVTGSGGGSHTAQTTA
                     TITTGEPPPPSSATKLLIEGVCVLGTDQEYVSIYNPTNAAVNLSNYYLTDALFTNQGY
                     WNIPDPSKRNQASSGGGAFNDWHVRFPDGATIAAGDTIAVSIQGSVAYQRAYMRKPHF
                     EIVATGITDDVTVPNMREAFTGSVPPAASGTSAHGLTNTGESVTLYYWDQLTDLVTDI
                     DYVVWGNDGGQFRYFNIKTGVMVDGPDADTAPSSYAADTPVAQQAFFPPADGTNLHAF
                     GNALRRTDFNEGTQHTTGGNGVGGRDETSENLPQTFSAAIPAVPPFVKSGGGGGTLMA
                     RPGGPYTGTVNQVIAFNGASSTPATGGSITAYNWTFGDGGSGTGANPTHTYTTAGTFT
                     VTLTVTGSGSTGTNSASTTATVTTDEPPPPSSATKLLIEAVCVLGTDQEYVSIFNPGT
                     QAVNLSNYYLTDAIFTNQGYWNIPDPSKRNQASSGGGAFNDWHVRFPDGASIAAGDTI
                     AVAIQGAAAYIRAYNRKPHFEIVAPGITDDATVPTMREAFTGAVPPAASGTSAHGLTN
                     TGESVTLYYWDQASDLVTDVDYVVWGNDGGQFRYFSIKTGVMVDGPDADTTPSTYKAD
                     TPVAQQAFFPPADGTNLHAFGNALRRTSFTEGTQKTTGGNGVGGRDETSENLPQTFSA
                     ALPAVPPFVKGSAPPPPGNLTAFISGTPLRGAVGQAISFNGSQSTPKTGETITNYAWT
                     FGDGGTATGAIASHAYATAGTFTATLTVTGSGGGTNSTSTQVSITVPSDGELQLVART
                     FMPTIGETYPITLDVELGRWQFLRIYDLEGREKRVLLDEQSAGVKKTILWDGRDEELM
                     LVSSGTYIIHLETRDEAGHIQSFSGPVVVARRLERN"
     gene            4547..6448
                     /locus_tag="VF720_16655"
     CDS             4547..6448
                     /locus_tag="VF720_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881043.1"
                     /translation="MRLWKRFSILTAMLGTVVVAADPASAVFTINEIRVDHTGTDVDE
                     YVEFRGTPSASLANHWLLVIGDNAAGTCGVLENIVDLSTYSIQADGYFALRISTGTPT
                     LTGYDATISGSIENSDNLTFLLVTGSTATPAQDLDTNNDGVLDATPWTGIVDQIGLDE
                     GTVPACATGDEYLYTATRIGPDGLNVPGHAWRCTDTGAWQIGQFDINGGVDTPGAANQ
                     SCTNPAPTIVAEHRNVCAPLGGQSVTVIDTVTAATSATLTYHINGGGPINVGMTQSGT
                     QWSGVIPAQVNGTVVTYQVVATNAQGMDTGTSRGYFVGTKTIASLRNPDPVTGVEPFR
                     FYGVRIAGTVTAAQGTFSAVNTDFWVQDGTGGIRIFKFGLPASSPALGDQVTIAGELD
                     QFNGLLEIENSPTCGDLEISIDNPGVAPAPVLINSCDLGEDTEGLFVKMEFPFADTTG
                     NNSANWGANKTYRIHDCLPDTVLLFIDTDTNIDGTPIVRTQMGITGISNQFDTVSPFT
                     AGYQIIPRSLSDIAYVYTTADVPGSAGGLKARLLPNAPNPFKDATTIRYDVAGSGNAR
                     GEVAVRINLYDVAGRLTRTLLNESKLPGSYELRVDASEMGDSPSGIYFYEMLIDNVSI
                     GTHKLLVTR"
     gene            complement(6600..7397)
                     /locus_tag="VF720_16660"
     CDS             complement(6600..7397)
                     /locus_tag="VF720_16660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX7881044.1"
                     /translation="MTPTTPRTDVLTGPLPLAGERHPLNRDYVRWLTRGNATALGTGL
                     VLFAAWWLWSGRKPVEPVARPVRLVRYAELSEPPSIAAPAPPQIQVQVAAARAVAAPT
                     IAIPEPVPMAEVQDTTIATQEEMAASDIPAPVTTDAFGVGSGDSLVVGGAGGGGGGIG
                     GEGEGDGHPSPEDFVAVESDPERLRMDTPVYPPVALQAGVEGTVMVQALVSKEGKVLK
                     VRVLEGPEMLHESAIACAKTAIFRPALAQNKPVEVWVVMPITFKLRK"
     gene            complement(7394..7897)
                     /locus_tag="VF720_16665"
     CDS             complement(7394..7897)
                     /locus_tag="VF720_16665"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7881045.1"
                     /translation="MADVGGGPARAQVKREGVISIKRPKRHDAVRIDMTPMVDVAFLL
                     LIFFMVTTVFRQPLAMEINMPEPDSAVKVPEGNVLTVDVDAADRMTWHLGPTLPAAAT
                     WPELDRVFAGALQRNPQLIVLVRVDRKARYATMVEMMDTLEDAGIERFSVVPKDDAPA
                     PAPAEQR"
     gene            complement(7900..8352)
                     /locus_tag="VF720_16670"
     CDS             complement(7900..8352)
                     /locus_tag="VF720_16670"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX7881046.1"
                     /translation="MAKASRAHGRVRIDMTPMVDVAFLLLIFFMSTTQFKAPESVAVV
                     LPASTSEVHVPQTGTIILTVTQAGDIYIADQQGTVSEPVPSDALFDTILDWRGRNPDA
                     VVILKGDRNADFGTISNVMETLARAKTLRFNLMTDLKRRDRGAPAGEN"
     gene            complement(8356..>8685)
                     /locus_tag="VF720_16675"
     CDS             complement(8356..>8685)
                     /locus_tag="VF720_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012507519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX7881047.1"
                     /translation="ASALETPILERNLSALSTIASIATLIGLLGTTIGMIRAFRAMST
                     AGAPDAVQLALGISEALVNTALGLITAIVGIVLHNWFSTRVEAFGSDMEEAGFETLQA
                     LRQRPER"
BASE COUNT         1383 a         2834 c         3015 g         1453 t
ORIGIN      
        1 cgggttgtgc caggccgaca gccagccgag atcgacgccg gtggccgcgc cacccagcgc
       61 gcgggcgcgc ggatcgacct cgagcgcctc gaaagacgcc cgggccggca gcgactgccc
      121 gagggacaac gtcaggacgg ccgccggcac ccagaaggcg cgggacccga agactcgccg
      181 catcgaaact ctcctcatcg cgtcggtgcg caccatggtc agttcctctc caggcggcgc
      241 gccacgacga cggggccgga aaagctctgg atgtgacccg cttcatcgcg ggtctcgagg
      301 tggatgatgt acgtgccgga cgacacgagc atcagttcct cgtcacggcc gtcccagaga
      361 atcgttttct tgacgcccgc tgactgctcg tcgaggagca cccgcttctc gcgaccctcg
      421 aggtcgtaga tgcgcaggaa ttgccagcgt cccaactcca cgtccagcgt gatcgggtag
      481 gtctcgccga tcgtgggcat gaatgtgcgg gccacgagct ggagttcgcc gtccgacggc
      541 accgtgatgg aaacctgcgt cgaggtgctg ttggtgccgc cccccgagcc ggtgaccgtg
      601 agagtggccg tgaacgtgcc ggccgtcgcg taggcgtggc tggcgatggc gccggtggcg
      661 gtgccgccgt cgccgaaggt ccacgcgtaa ttggtgatcg tctcgccggt cttcggagtg
      721 gactgcgaac cgttgaacga gatggcctgc ccgaccgcgc cgcggagcgg cgtgccggaa
      781 atgaaggccg tcaggttccc cggcggcggc ggcgcgctcc ccttcacgaa ggggggcacg
      841 gccggcagcg cggcgctgaa cgtctgggga aggttctcgc tggtttcatc gcggccgccg
      901 acgccgttcc caccggtggt cttctgcgtt ccctcggtaa agctggtccg gcggagcgca
      961 ttgccgaagg cgtgaaggtt ggtgccgtcg gccggcggga agaaggcctg ctgcgccacg
     1021 ggggtatcgg ccttgtaggt actgggcgtg gtgtcggcat cgggcccgtc caccatgacg
     1081 ccggtcttga tgctgaagta gcggaactgc ccgccgtcgt tcccccacac cacgtagtcg
     1141 acatcggtga cgaggtcgga cgcctggtcc cagtagtaga gcgtgacgct ttcgccggta
     1201 ttggtgaggc cgtgcgcgct cgtgccgctg gcggccggcg gtacggcgcc ggtgaaggct
     1261 tcccgcatcg tcggcacggt ggcgtcgtcg gtgattcccg gagccacgat ctcgaaatgc
     1321 ggcttccggt tgtaggcccg gatgtaggcc gcggccccct ggatcgccac ggcgatcgtg
     1381 tcgccggccg cgatggacgc gccatccggg aaacggacat gccagtcgtt gaacgccccg
     1441 ccgcccgagg acgcctggtt gcgcttagag ggatcaggga tgttccagta accctggttc
     1501 gtgaagatcg cgtcggtgag gtagtagttg gagaggttca ccgcctgcgt gccgggattg
     1561 aagatcgaca cgtactcctg gtcggtgccg agcacgcaga ccgcctcgat cagcagcttg
     1621 gtggccgacg acggaggggg cggctcgtcg gtggtgacgg tggccgtggt cgaggccgaa
     1681 ttggtgccgg tgctcccgga cccggtcacg gtgagcgtca ccgtgaaagt tccggccgtg
     1741 gtgtaggtgt gcgtggggtt ggcgccggtg ccgctgccgc cgtcgccgaa cgtccagttg
     1801 taggcggtga tgctgcctcc ggtggccggt gtggacgagg ctccgttgaa cgcgatgacc
     1861 tggttcacgg tgccggtgta cggaccgccg ggacgggcca tgagcgtccc gccaccgcca
     1921 cccgacttca cgaagggcgg gacggcgggg atcgcggcgc tgaacgtctg gggaaggttc
     1981 tcgctggtct catcacgccc gcccacgccg ttgccgccgg tcgtgtgctg ggtgccttcg
     2041 ttgaagtcgg tgcgccgcag ggcgttgccg aacgcatgga gattggtgcc gtcggccggt
     2101 gggaagaacg cctgctgcgc caccggcgta tcggcggcgt agctgctcgg cgccgtgtcg
     2161 gcatcaggtc catcgaccat cacgccggtc ttgatgttga agtagcggaa ctgccccccg
     2221 tcgttccccc acaccacgta gtcgatgtcg gtgacgaggt ccgtgagctg gtcccagtag
     2281 tagagcgtga cgctctcgcc ggtgttggtg aggccgtgcg cgctcgttcc gctggcggcc
     2341 ggcggcacgg agccggtgaa ggcctcgcgc atgttcggaa cggtgacgtc gtcggtgatg
     2401 ccggtggcca cgatctcgaa gtggggcttg cgcatgtagg cgcgctggta ggccacgctc
     2461 ccctggatcg acacggcgat ggtatcgccg gccgcaatgg tggcgccgtc cgggaaccgc
     2521 acatgccagt cgttgaacgc gccaccgccc gaggacgcct ggttgcgctt ggagggatca
     2581 gggatgttcc agtaaccctg gttcgtgaag agggcatcgg tcaggtagta gttcgagagg
     2641 ttcacggcgg cgttcgtcgg gttgtagatc gacacgtatt cctggtcggt cccgaggacg
     2701 cagactccct cgatgaggag cttggtggcc gaggaagggg gcggcggctc gccggtggtg
     2761 atggttgcag tggtctgagc ggtatggctt ccgcctcccg aacccgtaac ggtaagcatg
     2821 acggcaaagg tgccggccgt cgtgtaggtg tgcgtggggg tcgcaccggt gccggtcatg
     2881 ccgtcaccga agttccagtt gtaaccggtg atcgtttcgc cggtggccgg cgtcgaggcg
     2941 gatccgttga acgagaccgc ctggttcacg atgccggtgt aggggccacc gggctttgcg
     3001 accaggtcga cgatgaccgg gcctcccccc ttggcaaacg ggggcatggc gggttgatcc
     3061 ttgctccagg tgacggccag gttctcactg gtctcgttgc ggccaccgat gccgttgccg
     3121 cccgacgtgg cctgcgtccc ctcgttgtaa tcctcacgcc ggaaggcgtt tccgaacgtg
     3181 tgggcctgtg cggcctcggc cggcacgatg aacgactgct gggccaccgg cgtgtcggcg
     3241 gcgtaggcgc tggcgttgga atcgccgtcg ggaccgtcga ccgtcacgcc ggtcttgatg
     3301 ttgaagtagc ggaacatccc ggtgtcgtta ccccagacga ggtagtcgac gtcgatgacg
     3361 agatccgact gctggtccca gtaataaagg gtgacgctct caccggagtt ggtgaggccg
     3421 tgcgccgtgg cgccgttggc cgacgccggc accgagccgg ggaaggcctc gcgcatgttg
     3481 ggcacgctgg cgtcgtcggt gagcccggcc gccaggattt cgaaggcggg cttgcgggag
     3541 taggcccgca cgaaggcggc gcttccctgg atcgaaacgg ccaccgtgtc gccggccgcg
     3601 atcatggagc ccgccgggaa gcggacatgc cagtcgttga acgtgccgcc acccgaggag
     3661 accgagttgc gcttggtcgg gtcgggcagg aaccagtacc cctggttcgt gaagagggcg
     3721 tcggtcaggt agtagttgga gaggtcgacc gaggcattcg tcgggttgta caccgccacg
     3781 tattcctggt cggtgccgag gatgcagacg gattgaagga ggagcttggc gggagtctgg
     3841 gccgaaacag gcgacgggag cgccgacaga gcgaggaccg cgatcgggag cagggcgagc
     3901 agcccgagcg gcgccgccct ccgcggacgg taaaacctca tgattccgaa cctttcgggc
     3961 aggcgtggcc cgggggacgg gatgatggac gcgggaccag ctccccccat ggtgctggtc
     4021 gagcgctccg ccgcgtggac aatggcggca ttcgaggcct cgcagggccg tggagtgtcg
     4081 gttcgggagc gggttggttc cctgaagacg acgccagtgg cctggcgtga cgtccgggat
     4141 gagcgaaatg caccacggcc gccccgtgga tccccgacag gcggcgcaat atagcaacgc
     4201 cctccggggc tgtccacttt tgactgccgt ggacggtgtg ccaacgggtt atcgggtccg
     4261 gaacgcgccg gcgggccgcg tgaccgcgcc ccgggcacct ccaccaccac gtggaatccc
     4321 ccgtcccggc cacgccgacg agggcaaccg cccggcaccg accggggggg gcccttgccg
     4381 ggtgccagcc ccgccatcta cccgaaattg gtggaaagac gacccgcccc ggactaaact
     4441 gttgagcgtt cagatttccg atctgccggt acgcaccggc ttgccactcc ccctgttcgg
     4501 gggaactcat tccatttctc aggacatccg agggggtacc ggcaccatga ggctttggaa
     4561 acggttttcc attctgaccg ccatgctggg aaccgtcgtc gtcgcggccg atccggcttc
     4621 cgccgtcttc acgatcaacg aaatccgagt cgaccacacg ggtaccgacg tggatgaata
     4681 cgtggaattc aggggaacgc cgagtgcttc tctcgccaac cattggctcc tggtgattgg
     4741 cgacaatgcg gcggggacct gcggtgtcct cgagaacatc gtcgatctct cgacctactc
     4801 gattcaggcc gacggctatt tcgcgctgcg catcagcacc ggcacgccca ctctcacggg
     4861 gtacgacgcc acgatcagcg gctcgatcga gaacagcgac aacctgacct tcctgctcgt
     4921 caccggcagc acggccaccc ctgcgcagga cctcgacacc aacaacgacg gcgttctgga
     4981 cgccaccccc tggaccggca ttgtcgatca gatcggactc gatgaaggga ccgtcccggc
     5041 ctgcgccacg ggcgacgaat acctctacac ggcaactcgc atcgggccgg acggcctcaa
     5101 tgtgcccggc catgcctggc ggtgcaccga caccggcgcc tggcagatcg gccagttcga
     5161 tatcaacggc ggcgtcgaca cgcctggtgc ggccaaccag agctgcacca accccgcccc
     5221 gaccatcgtg gccgagcacc gcaacgtctg tgcgccgctc ggcggccaga gcgtgacagt
     5281 catcgacacg gtcacggcgg ccacgtcggc cacgctcacc taccacatca acggcggtgg
     5341 gccgatcaac gtggggatga cccagtccgg cacgcagtgg tcgggtgtga tcccggccca
     5401 ggtcaatggc acggtcgtca cctaccaggt ggtggccacc aatgcccagg ggatggacac
     5461 cggcacgagc cgtggctact tcgtcggcac gaagaccatc gccagcctgc ggaatcccga
     5521 tccggtgacc ggcgtggagc cgttccgctt ctacggcgtg cgcatcgccg gcaccgtgac
     5581 cgcagcccag ggcaccttca gcgccgtcaa cacggatttc tgggtgcagg acggaaccgg
     5641 cggcatccgc atcttcaagt tcggcctgcc ggcctccagc ccggccctgg gcgaccaggt
     5701 gaccatcgcg ggcgagctcg accagttcaa cggcctcctc gagatcgaga attcccccac
     5761 ctgcggcgac cttgagatct cgatcgacaa cccgggcgtg gcgccggcgc cggtgctcat
     5821 caactcctgc gacctcggcg aagacaccga agggctcttc gtgaagatgg agttcccgtt
     5881 cgccgacacc accggcaaca acagcgcgaa ctggggcgcc aacaagacgt atcgcatcca
     5941 tgactgtctg ccggacaccg tgctcctctt catcgacacc gacaccaaca tcgacggcac
     6001 cccgatcgtc cgcacccaga tggggatcac cggcatctcg aaccagttcg acacggtgtc
     6061 gcccttcact gcgggctacc agatcattcc gcgcagcctg tccgacatcg cctacgtgta
     6121 caccaccgcg gacgtccccg gctcggcggg tggcctcaag gcccgcctgc tgccgaacgc
     6181 gccgaatccg ttcaaggacg ccaccaccat ccgctacgac gtggccggtt ccggtaacgc
     6241 ccggggcgaa gtggccgtcc gcatcaacct gtacgacgtg gccggccgtc tcacccggac
     6301 gctcctcaat gaaagcaagc tcccgggttc gtacgagctg cgcgtcgacg ccagcgagat
     6361 gggcgattcg ccgagcggca tctacttcta cgagatgctc atcgacaacg tctcgatcgg
     6421 cacgcacaag ctgctcgtca cgcggtaacg cgggacgaag gtcccgccag cgggcctgac
     6481 atcgtcacca tgaaaagggg gccggaggaa actccggccc ccttttctct tgctgatgtc
     6541 gaaagagtat tgctgatccg gaaagccgat caggaaactg gtcccgaggc ctgtcggtcc
     6601 tacttccgca gcttgaacgt gatcggcatc accacccaca cctcgaccgg cttgttctgg
     6661 gccagggccg gccggaagat ggccgtcttc gcgcaggcga tcgccgattc gtggagcatc
     6721 tccggccctt cgagcactcg gactttgagg acctttccct ccttgctcac cagggcctgg
     6781 accatcacgg ttccttcgac gccggcctgc agcgccacgg ggggatagac cggcgtgtcc
     6841 atccgcaggc gctcgggatc ggactcgaca gccacgaagt cttcgggtga cgggtggcca
     6901 tccccctctc cttctccgcc gattcccccg ccgccaccgc ccgccccgcc gacgacgagc
     6961 gagtcgccgc tgccgacgcc gaacgcatcg gtggtcaccg gcgcgggaat gtcggaagcg
     7021 gccatctctt cctgggtggc gatggtcgtg tcctgcactt cggccatcgg tacgggctcg
     7081 gggatggcga tggtcggtgc cgccacggct cgtgcggccg ccacctgcac ctggatctgg
     7141 ggcggcgcgg gggcggcaat cgacggcggt tcggagagct ccgcgtagcg caccagtcgg
     7201 acggggcgag ccaccggttc aacgggcttc cggccgctcc agagccacca ggccgcgaac
     7261 agcacgaggc cggtgccgag cgcggtggcg tttccccgtg tgagccagcg gacgtaatcg
     7321 cgattgagcg gatgccgctc ccccgcaagc ggaagcgggc cggtgagcac gtcggtgcgc
     7381 ggcgtggtcg gggtcatcgt tgctccgccg gcgcgggcgc gggcgcgtcg tccttcggca
     7441 ccacgctgaa gcgctcgatc ccggcgtctt ccagcgtgtc catcatctcg accatggtgg
     7501 cgtagcgggc cttccgatcc acccgcacga ggacgatcag ctgtggattg cgctgcagcg
     7561 cgcctgcgaa gacgcggtcg agctccggcc aggtggcggc cgccggcagg gtgggcccga
     7621 ggtgccaggt catccggtcg gcggcgtcca cgtccaccgt gagcacgttc ccctccggca
     7681 ccttcacggc cgagtcgggc tccggcatgt tgatctccat ggccagcggc tggcggaaga
     7741 ccgtcgtcac catgaagaag atgaggagaa ggaaggccac gtcgaccatc ggggtcatgt
     7801 cgatccggac ggcgtcgtgt cgcttcgggc gcttgatgga gatgacgccc tcacgcttga
     7861 cctgggccct ggcggggccg ccgccgacgt cggccatggt cagttctccc cggccggcgc
     7921 gccacgatcg cggcgcttga ggtcggtcat caggttgaag cgaagcgtct tggccctggc
     7981 cagcgtttcc atcacgttcg agatcgtgcc gaagtcggcg ttccggtcac ccttcaggat
     8041 gacgacggca tcgggattgc ggccgcgcca gtcgaggatg gtgtcgaaga gcgcatcgga
     8101 gggcaccggc tcggaaaccg tcccctgctg gtcggcgatg tagatgtcgc cggcctgcgt
     8161 caccgtcagg atgatcgtgc cggtctgcgg cacgtgcact tccgacgtcg aggccggcag
     8221 caccaccgcc accgactcgg gcgccttgaa ctgcgtggtg gacatgaaga agatgaggag
     8281 caggaacgcc acgtcgacca tgggggtcat gtcgatgcgg acgcgaccgt gggcccggct
     8341 ggccttggcc atggctcagc gctccggacg ctgcctgagc gcctggagcg tttcgaaacc
     8401 ggcctcttcc atgtcgctgc cgaacgcttc cacccgcgtc gagaaccagt tgtgcagcac
     8461 gatcccgacg attgcggtga tgaggccgag cgccgtgttc accagcgcct cggagatgcc
     8521 gagcgccagc tgcacggcgt cgggcgcgcc ggccgtcgac atggcgcgga aggcgcggat
     8581 catgccgatc gtggtgccga ggaggccgat gagcgtggcg atggaggcga tggtcgagag
     8641 cgcggagagg ttgcgctcga ggatgggcgt ctcgagggcg ctggc
//
LOCUS       DASVOD010000779         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_573696, whole genome shotgun sequence.
ACCESSION   DASVOD010000779 DASVOD010000000
VERSION     DASVOD010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573696"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1289
                     /locus_tag="VF720_16680"
     CDS             153..1289
                     /locus_tag="VF720_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEX7881048.1"
                     /translation="MRMPMPVLPLTAVFDDLPDPRRDTANKLHALTDLLAIATCAVIG
                     GAESWEAVAEYGRTKEAFFRRFLPLANGIPSPDTFERVFAKLAPGAFARAFGRWMAAA
                     CEAAGLVPIAIDGKSARRAKRNTATGCLTVVSAWATENRLTLGQVAVPDGSNEIGVIP
                     DLLRTLDLAGAIVTIDAAGCQVENARLIRGQGGHYLLAVKDNQPTLRAAVGRVAEAAA
                     AADFAGVPIGEAVGEGHGRQEERYVSVFGTPAGIPADWPDVAAVVQVNREREVGGKRT
                     CTTHYYLTSYAGTAAEMAAFVRGHWGIENGLHWVLDVVFREDDSRVRAGHAGENLAMV
                     RRVAVSLLRRAPGKGTTPTKRLKAGWDDDYLLQVLQGIPALVVR"
BASE COUNT          236 a          495 c          573 g          220 t
ORIGIN      
        1 ccacgtatgg ggcgtgatcc cccgctcgct ttcctccaga aacgatttga gcattgggcg
       61 ggcgtccctg tttacgcccc accagatgcg gcagggctac cgcactacga accgctgacg
      121 gcatagtggc ttgaggctac accgagggca tcatgaggat gcccatgccg gtactgccat
      181 tgacggcggt gttcgacgac ctgcccgacc cgcgccggga cacggccaac aagctccatg
      241 ccctgaccga cctcctcgcc atcgccacgt gcgcggtcat cggcggggcc gagtcgtggg
      301 aggccgtcgc cgagtacggg cggaccaagg aggcgttctt ccgccggttc ctgccgctgg
      361 ccaacggcat cccgagcccg gacacgttcg agcgggtgtt cgccaaactc gccccggggg
      421 cgttcgcccg ggcgttcggc cggtggatgg cggcggcgtg cgaggcggcc ggtctggtcc
      481 cgatcgccat cgacgggaag tcggcccggc gggccaagcg gaacaccgca accgggtgcc
      541 tgacggtggt gagcgcgtgg gccaccgaga accggctgac cctcggccag gtggccgtcc
      601 cggacgggtc gaacgagatc ggggtgatcc ccgacctgct gcggaccctg gacctggccg
      661 gggcgatcgt gacgatcgac gccgccggct gccaggtcga gaacgcccgg ctcatccggg
      721 gccagggcgg gcactacctg ctggcggtga aggacaacca gccgaccctc cgggcggccg
      781 tcgggcgggt ggccgaggcg gcggccgcgg cggacttcgc cggggtgccg atcggggagg
      841 cggtcgggga gggccacggg cggcaggagg agcggtacgt cagcgtgttc gggacgccgg
      901 cggggatccc ggcggactgg ccggacgtgg cggcggtggt gcaggtgaac cgggagcggg
      961 aggtcggtgg gaagcggacg tgtacgaccc actactacct caccagctac gccgggacgg
     1021 cggccgagat ggcggccttc gtccgggggc actgggggat cgagaacggg ctccactggg
     1081 tactcgacgt ggtgttccgg gaggacgaca gccgggtccg ggcggggcac gccggcgaga
     1141 acttggcgat ggtccggcgg gtcgccgtgt cccttctccg acgggcaccg ggcaagggaa
     1201 caacgccgac caagcggctc aaggccgggt gggatgacga ctacctactc caggtacttc
     1261 aaggaatccc ggcactcgta gtgcggtagc cctgcggtag ccctgaacgg ctgggtgatg
     1321 gatttggcgc gcgagctgaa agaagctggt tacacgattg acgccaacaa gcttctttag
     1381 agcgcacagg cagcccgtcg tttctgtcac cccctccacg caagtgaccc gtcgtagcgg
     1441 atcgtccgca ccggcgcggt tgggtcgaac ggtttcacgt ctggcaggga atcgccgcac
     1501 ccctcagctg ccggcgctcc tggc
//
LOCUS       DASVOD010000780         7730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_416143, whole genome shotgun sequence.
ACCESSION   DASVOD010000780 DASVOD010000000
VERSION     DASVOD010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7730
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416143"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF720_16685"
     CDS             complement(<1..253)
                     /locus_tag="VF720_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX7881049.1"
                     /translation="MRALVYHGSKDVRVDEVDEPRLMADDDLILRVTATAICGSDLHI
                     YRGKIPKMEDGDILGHEFMGIVEEAGRGVSAVKKGDRVVI"
     gene            complement(558..3893)
                     /locus_tag="VF720_16690"
     CDS             complement(558..3893)
                     /locus_tag="VF720_16690"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX7881050.1"
                     /translation="MNRSTPIAGSFKAAALTAAVIAVLALTAHAADKKDDKPKDDGKP
                     AARLTSSVLSGFKFRSIGPATYSGRVIDIAIHPSDLNTWYVAVACGHLWKTTNHGISW
                     TPMLDDQGSYSFGCVTIDPNDPLTVWVGSGENNSQRSVGYGDGVYLSKDGGTSWNNVG
                     LKNSEHIGMIAVDPRNSKTVYVAAMGPLWNSGGDRGIYKTVDGGTTWERVLDIDENTG
                     FNEVHLDPTNPDVIYATAYQRGRRVWTLVNGGPGSGLHKSTDGGKTWTKLTNGLPKEE
                     MGRLGMAISPKNPRHLTVLVEAANSGGGTFRSDDGGLNWSKAGPYTSGGAQYYQELVP
                     DPSDDDRLYSMDTFLQVTNDGGKSWERAGEKFKHVDNHAVWIDPGDSDHLLVGCDGGL
                     YETYDRCQSFSFFGNLPITQFYKIAVDNDLPFYNIYGGTQDNYSFGGPSRTTNNHGIV
                     NADWYVTQGGDGFQTQVDPTDPNIVYSQAQHGELTRYDRKSGEGIDVQPQADPGQDTP
                     RWNWDSPLLISPHLHTRLYFAANRLYRSDDRGDNWRPISGDLTRQLDRDTLPLMGRTW
                     SIDAVARHGSTSQYGNIVALAESPKKEGLLYVGTDDGLIQVSDNGGESWRKIEQVGNV
                     PELTYVSRLTASSHDEGRVYAAFNNMKMGDFKPYLFRSDDRGGSWHSIAGDLPVRGGV
                     WAIVEDPVRPDLLFVGTEFGLYVTFDGGTKWLPMKAGLPVVAVRDIAIQARENDLVLG
                     TFGRSFYVLDDYAPLRALGEKELEAEGATFAVRPALMYFPSSPLGLKDKAFQGEAFYA
                     APNPPFGAVLTWYLRDERKTRKKLRQESEKALAKEMKPIPRPTWEELTAEGREEDPYV
                     EITIKDAEGQVVRRLKGGAGSGIQRVAWDLRYPAPDPVDLSPPDNSSPFGSPPAGPVV
                     LPGTYRASFSVVAYGLTTPIGNEQPIEVKALNSGTLAATNPADVAEFRTKTARLQRAL
                     LGATSALGEAQRRVDHLKKALVDAPAADPAWAADVRTLDQAFKDLKVRFSGDPVKSAK
                     NFPQPVSLTSRVNRIVEGHWASTSMVTGTLRKNYDIAAADFETALADLHKLIDVELAG
                     LEEKAEKAGAPWTPGRVPTWTRE"
     gene            complement(3922..4833)
                     /locus_tag="VF720_16695"
     CDS             complement(3922..4833)
                     /locus_tag="VF720_16695"
                     /inference="COORDINATES: protein motif:HMM:NF015509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEX7881051.1"
                     /translation="MLLLRLFVDNLLPAFLAAGAGTFLAWRLKVPTAPLSRTALFLFS
                     PCLIFKLVMAQPADPAPLLKMAGYVLTTLPIVGILAWITARALRLDRSMTAAMVLVAL
                     LPNAGNLGLAVNLFAFGKEGLAQAGLFFIVSALWSYSAGVVVAAMGKVGIRQAILSLV
                     KLPALWAALVAFIFLGMGWRLPTPVHRSVDMLADAAIPIFLVVLGMQLVGVDWKARRG
                     PVVAATALRLLAGPAVALAMAVAWGLDGAARQAAVFQASMPTAVFASVIMSEFEGEAE
                     FTSAVILTTTILSPLTLTPLMALLGAR"
     gene            complement(4840..6030)
                     /locus_tag="VF720_16700"
     CDS             complement(4840..6030)
                     /locus_tag="VF720_16700"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX7881052.1"
                     /translation="MTKPRTGSVFITGGLQRKALAAALALKQRGVEVTVGDPSHLALS
                     LWSLRVDHRALYPDPEQSEERFIDWMVRRMKSHPVDLLFPTGGEGEINAIHKHRDRFD
                     GIVTVGLSPAHLFEVTEDKGTLYDRAVKAGVAAPLTWQPATVEEALDRAGECTYPALI
                     KPRRGSGGRGIVHVANADALRAAYPDVAARHVKPLIQEFVPSSGVGLGVAALYDLQAR
                     PVTLFSYKRLREFPIGAGPSTFTESTRDPELLDAARRILDELGWQGLAMAEFRRDARD
                     GKAKLIEVNGRFWGSARLPIVSGLNFPWLYFQLMTTGKVDPPPAYKEGVRCRWLFPGD
                     FLHLVTNKKRWKMDPPFFQFFGKDLYYDPPIIPDPFPLMGQVAWAAWHAMHLPTLRKY
                     LHRR"
     gene            complement(6132..6554)
                     /locus_tag="VF720_16705"
     CDS             complement(6132..6554)
                     /locus_tag="VF720_16705"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX7881053.1"
                     /translation="MTAYVDSSVLLRLVLQQSNRLSEWDNVTRPVTSRLSEVECLRTL
                     DRLRLDEMLDDLGLAKNRRALDQLARQLDFIELTRPVLSRAGGPMPTRLRTLEAIHLA
                     SALQWRSSRKESIVMATHDRALGIAARASGLEVVGVIF"
     gene            complement(6551..6817)
                     /locus_tag="VF720_16710"
     CDS             complement(6551..6817)
                     /locus_tag="VF720_16710"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX7881054.1"
                     /translation="MIKSVKIAPLRARFSEFLRRVRSGEEFIVMDRDRPVARLVPIEP
                     DDDDLVVTPGAGAYARMGDMPLPDAVQLSEDPVRYLLEDRESGL"
     gene            complement(6941..>7730)
                     /locus_tag="VF720_16715"
     CDS             complement(6941..>7730)
                     /locus_tag="VF720_16715"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HEX7881055.1"
                     /translation="GTRLAVGSEDGFQLFDITNPVAPQLVGTLDLGQSIGAVVFDGSF
                     VFAEASGFEVLAIDLTTPANPVVAADVLLDDTSQALHLDGDRLYVGGYGTMMHVVDVS
                     NPTEPSVLGWHFAPVDGDIRGLASYSGVIYAAASYDGMYLLPPQCEAASGVPTPGPAV
                     ARFVVRPPFPNPSRGTVNIDIEPGELPLASGAEAPARVLRVSIVDVAGRLVRHWPELK
                     LESDEVRLTWDGRDNAGLAVPAGVFFVRVSDGQTRLAQRLVRVE"
BASE COUNT         1293 a         2545 c         2622 g         1270 t
ORIGIN      
        1 ggatgacgac acggtctccc ttcttcacgg cgctgacgcc gcgcccggcc tcctccacga
       61 tccccatgaa ttcatgtccg aggatgtcgc cgtcttccat cttcgggatc ttcccgcggt
      121 agatgtgcag gtcggagccg cagatggccg tggccgtcac ccttaggatg aggtcgtcgt
      181 ccgccatcag gcggggttca tcgacctcat cgacccggac atccttcgag ccgtgataca
      241 cgagtgcgcg cattgcttcc tcctctcggg ccagaggacg ggagtcgacg caagcgcgat
      301 gccatggcgg gcgccggagg gccggaggcg ttgtgatggc cggcgggccg aaacgtgggg
      361 acggaaccgg cggccgggcg gggagccacc ggccacgggg gtaaggtcat gcccgaacgg
      421 ccgccgtggt gggcgctacc tttcgtcccg gcgtgaaaaa gaacgccccc gcgatgtcac
      481 gacgatcatc gcggggacgt caggaggtcg tgccgtaccg gcgcagggcc ggtgtcgatt
      541 cgggtgacgt gccggtctta ttccctcgtc caggtcggca cgcgccccgg cgtccagggc
      601 gcgccggcct tctcggcctt ttcctcgagc ccggccagct ccacgtcgat cagcttgtgg
      661 aggtcggcca gcgccgtctc gaaatcggcg gcggcgatgt cgtagttctt ccggagcgtg
      721 ccggtcacca tcgaggtcga ggcccagtgc ccttccacga tccggttcac gcgcgaggtc
      781 agcgacacgg gttgggggaa attcttggcc gacttcacgg ggtcgccgct gaagcgcacc
      841 ttcaggtcct tgaacgcctg gtcgagcgtc cggacgtcgg cggcccaggc ggggtcggcg
      901 gccggggcat ccaccagggc cttcttcagg tgatccacgc ggcgctgggc ctcgccgagc
      961 gccgaggtgg cgccgaggag ggcgcgctgg aggcgggcgg tcttcgtccg gaattcggct
     1021 acgtcggccg ggttcgtggc ggcaagggtg ccgctgttca gcgccttcac ctcgatcggc
     1081 tgctcgttgc cgatcggcgt cgtgaggccg taggccacca ccgaaaagga cgcgcgatac
     1141 gtgccgggga gcacgaccgg gccggccggc ggtgagccga acgggctcga gttgtcgggc
     1201 ggggatagat ccaccgggtc gggggccggg tagcggagat cccaggccac gcgctggatg
     1261 ccggagcccg cgcccccctt cagccgccgg acgacctgtc cctcggcgtc cttgatcgtg
     1321 atctcgacgt acggatcctc ctcccgcccc tcggccgtca gctcctccca ggtcggacgg
     1381 gggatcggct tcatctcctt ggccagcgcc ttctccgatt cctggcgcag cttcttcctc
     1441 gttttccgtt cgtcgcgcag gtaccaggtg agcacggcgc cgaacggggg gttgggcgcc
     1501 gcgtagaacg cctcaccctg gaaggcctta tcttttaatc cgagcggcga cgacgggaag
     1561 tacatgagtg ccggccgcac cgcgaacgtg gccccctcgg cctccagctc cttttcgccg
     1621 agggcacgca gcggcgcgta atcgtcgagc acgtagaagc tgcgcccgaa cgtacccagc
     1681 acgaggtcat tctcgcgcgc ctggatggcg atgtcgcgca ccgccaccac cggcagcccg
     1741 gccttcatcg gcagccactt cgtgccgccg tcgaacgtca cgtaaagccc gaactcggtg
     1801 ccgacgaaga ggaggtcggg gcgcaccgga tcttccacga tggcccatac gccgccgcga
     1861 acgggaaggt ctccggcgat ggagtgccag ctcccgcccc ggtcgtcact ccggaagagg
     1921 tacggcttga agtcgcccat cttcatgttg ttgaaggcgg cgtacacgcg cccctcgtca
     1981 tggctcgacg cggtgagccg gctgacatag gtgagctcgg ggacgttgcc gacctgctcg
     2041 atcttccgcc agctttcgcc gccgttgtcg ctcacctgga tgaggccgtc gtcggtgccc
     2101 acgtagagga gcccttcctt cttcgggctc tcggccagcg ccacgatgtt gccgtactgc
     2161 gaggtggagc cgtggcgcgc cacggcatcg atgctccagg tgcgtcccat gaggggaagc
     2221 gtgtcgcggt cgagctggcg cgtcaggtcg ccggaaatgg gccgccagtt gtcgccgcgg
     2281 tcgtcgctcc ggtagaggcg gttggcggca aagtagaggc gcgtgtggag gtgcgggctg
     2341 atgaggagcg gcgaatccca gttccagcgg ggcgtgtcct ggccggggtc ggcctgcggc
     2401 tgcacgtcga tcccctcgcc gctcttcctg tcgtagcgag tcagctcgcc gtgctgggcc
     2461 tgcgagtaga cgatgttcgg atcggtcgga tcgacctgcg tctggaagcc gtcccccccc
     2521 tgcgtgacgt accagtcggc gttcacgatg ccgtggttgt tggtggtgcg cgaggggccg
     2581 ccgaacgaat agttgtcctg cgtgccgccg tagatattgt agaaggggag gtcgttgtcc
     2641 accgcgatct tgtagaactg ggtgatcggc aggttgccga agaacgagaa cgactggcag
     2701 cggtcgtacg tttcgtagag gccaccgtcg cagccaacca gcaggtgatc ggaatcgccg
     2761 ggatcgatcc acacggcgtg gttgtcgacg tgcttgaact tctcgccggc gcgctcccag
     2821 ctcttgccgc cgtcattcgt cacctggagg aacgtgtcca tcgagtagag gcggtcgtcg
     2881 tcggacggat cgggcaccag ctcctggtag tactgcgcgc cgcccgacgt gtacgggccg
     2941 gccttcgacc agttgaggcc gccgtcatcg gagcggaacg tgccgccgcc cgagttcgcg
     3001 gcttcgacca gcaccgtcag gtgccgcggg ttcttcggcg agatggccat gccgaggcgc
     3061 cccatctcct ccttcggcag cccgttggtc agctttgtcc acgtcttgcc gccgtcggtg
     3121 gacttgtgga gccccgagcc gggtccgccg ttcaccaggg tccagacgcg tcggccgcgc
     3181 tggtaggccg tggcgtagat gacgtccggg ttggtcggat cgagatgcac ctcgttgaaa
     3241 ccggtgttct cgtcgatgtc gaggacgcgc tcccaggtcg tgccgccgtc cacggtcttg
     3301 tagatgccgc gatcgccgcc cgaattccag agcggcccca tggccgccac gtacacggtc
     3361 ttcgaattgc gcggatcgac cgcgatcatc ccgatgtgct cggaattctt gagtcccacg
     3421 ttgttccacg acgtgccgcc gtccttcgag agatacacgc catcgccgta acccaccgag
     3481 cgctggctgt tgttctcgcc gctccccacc cacaccgtga gcgggtcgtt cggatcgatc
     3541 gtcacgcagc cgaaagagta cgacccctgg tcatcgagca tcggcgtcca cgagatgccg
     3601 tggttggtcg tcttccagag atgaccgcag gccaccgcca cgtaccaggt gttgaggtcc
     3661 gatgggtgga tggcgatgtc gatgaccctc cccgaatagg tggccggccc gatcgagcgg
     3721 aacttgaagc cggagagaac ggacgaggtg aggcgcgcgg cgggcttgcc gtcgtccttc
     3781 ggcttgtcgt ccttcttgtc ggcggcatgg gcggtcagtg ccagtacggc aatcaccgcc
     3841 gcggtcagtg cggcggcctt gaagctcccg gcgatcgggg tcgaacggtt catgggggaa
     3901 atcctcctcg gcaagccggc attatcgcgc tccgaggagc gccatcaaag gagtcagtgt
     3961 caggggggaa agaatggtgg tcgtgaggat caccgccgag gtgaattcgg cttctccctc
     4021 gaattcactc atgatcacgc tcgcaaaaac cgccgtcggc atggaagcct ggaagaccgc
     4081 cgcctgccgg gccgccccgt cgagccccca cgccaccgcc atggccaggg ccacggccgg
     4141 tcccgccaga aggcggaggg cggtggcggc cacgaccggc ccccgccggg ccttccagtc
     4201 gacccccacg agctgcatcc cgaggacgac caggaagatc gggatggccg cgtcggccag
     4261 catgtccacc gaacggtgca ccggagtcgg gagccgccac cccatcccca ggaagatgaa
     4321 ggccacgagc gccgcccaga gggccggcag cttgacgaga gacaggatcg cctggcgaat
     4381 cccgaccttg cccatggccg ccaccaccac gccggccgaa tacgaccaga gcgccgacac
     4441 gatgaagaag agccccgcct gcgccagccc ctccttgccg aaggcaaaga gattgacggc
     4501 cagccccagg ttgccggcat tcggcagcag cgccaccagc accatcgccg ccgtcatcga
     4561 gcgatcgagc cgcagcgccc gtgccgtgat ccaggccaga attcccacga tcggcaacgt
     4621 cgtgaggacg tagcccgcca tcttcaggag cggagccgga tcggccggct gcgccatcac
     4681 cagcttgaag atgaggcagg gcgagaaaag aaagagcgcc gtccgcgaca gcggcgccgt
     4741 cggcaccttg aggcgccagg cgaggaacgt gccggcgccc gcggccagga aggcggggag
     4801 gaggttgtcg acgaagaggc ggaggaggag cacgatcgat cagcgccggt ggaggtactt
     4861 gcgcagcgtg gggagatgca tggcgtgcca cgcggcccag gccacctgtc ccatgagcgg
     4921 gaacgggtcg gggatgatcg gcgggtcgta atagagatcc ttgccgaaga actggaagaa
     4981 gggcgggtcc atcttccagc gcttcttgtt cgtgacgagg tgaaggaaat cgcccgggaa
     5041 gagccagcgg cagcgcacgc cctctttgta ggcgggcggc ggatcgacct tgccggtcgt
     5101 catgagttga aaatagagcc aggggaagtt gagccccgaa acgatcggca gtcgtgccga
     5161 cccccagaag cgcccgttca cctcgatgag cttggccttc ccgtcccgcg cgtcgcgccg
     5221 gaactcggcc atggccagcc cctgccagcc aagctcgtcc agaatgcgcc gcgccgcgtc
     5281 gagcagctcc ggatcgcggg tcgactcggt aaaggtggat ggccccgccc cgatcggaaa
     5341 ctcgcggagg cgcttgtagc tgaagagtgt caccggccgg gcctgtaagt catacagggc
     5401 cgccacaccg agcccaacgc cggaagacgg cacgaattcc tggatcaacg gcttgacgtg
     5461 ccgtgcggcg acatccggat acgccgcgcg cagcgcgtcc gcattggcca catgcacgat
     5521 gccccgcccg ccgctcccgc gccgcggctt gatgagtgcc gggtaggtgc attcgccggc
     5581 ccgatcgagc gcttcttcca ccgtggccgg ctgccaggtg aggggcgccg ccacgcccgc
     5641 cttgaccgcc cggtcgtaca gcgtcccctt gtcctcggtc acctcgaaga ggtgggcggg
     5701 ggagaggccg accgtcacga tgccgtcgaa acggtcgcga tgcttgtgga tggcgttgat
     5761 ctctccctcg ccgccggtgg ggaagaggag gtcgaccggg tgcgatttca tccgccggac
     5821 catccagtcg atgaagcgct cctccgactg ctccggatcg ggatagagcg cccggtggtc
     5881 gacgcgaagg gaccagaggg aaagtgcgag atgggagggg tcgccgacgg tgacttccac
     5941 gccgcgctgt ttcagcgcca gcgcggccgc cagcgctttc cgctggaggc cacctgtgat
     6001 gaagacagac ccggtgcggg gcttcgtcat gtacggggag ggcgttccgc ggggggcgtc
     6061 atgcggacga tgacgaaaga actcattgga aaataataag atagtggagc agtggttggt
     6121 agggcctcgt ctcagaagat gacgccgacg acctcgagac ccgacgcccg ggccgcgatc
     6181 ccaagggcac ggtcgtgcgt tgccatgacg atcgattcct tccgactcga tcgccactgc
     6241 agcgccgacg ccaggtggat ggcctccagc gttcgcaggc gggttggcat gggtcctccg
     6301 gcgcgactca gcacgggacg cgtcagctcg atgaaatcaa gctgacgggc gagttgatcc
     6361 agtgcccgtc gattcttcgc cagcccgagg tcatccagca tctcatccag tcgcaggcgg
     6421 tcgagggtcc ggaggcactc cacctcgctg aggcggctcg ttaccggacg cgtcacgttg
     6481 tcccattccg acagccggtt cgactgctgg agcaccaatc gcagaaggac cgacgagtcg
     6541 acataggcgg tcacagcccg gactcccggt cttccagcag gtatcgcacc ggatcttcag
     6601 acagttgcac cgcatcgggg agcggcatgt cgcccatacg cgcataagca cccgcgcccg
     6661 gcgtcacgac caggtcgtcg tcgtccggct cgatgggaac cagtcgggcg accggacgat
     6721 cacggtccat gacgatgaac tcctctccgc ttcgcacgcg ccgcaggaac tcgctgaaac
     6781 gggcccgcaa cggggcgatc ttgaccgact tgatcatgtc atgaccatgt caggtcaagt
     6841 tgatgtccgc aagaaccttt gtatgaaatg tctttgatta caacgaaatg caggcgcagg
     6901 tttacataat ggctcggccc gaaccgagtc tgccctcgtc ctactcgacc cggaccaacc
     6961 gctgcgccag tcgggtctgc ccatcgctca cccggacgaa aaacacgccc gccggaacgg
     7021 ccaggccggc attgtcccga ccatcccagg tgagtcggac ctcgtcagac tcgagcttca
     7081 actcgggcca gtgccgcacg agtcggccgg cgacatccac gatcgacacg cggaggaccc
     7141 gggcgggtgc ctcggcgccc gaggcgagcg gaagctcacc cggctcgatg tcgatgttca
     7201 cggtgccccg gctcggattg gggaacggcg ggcgcacgac gaacctcgcg accgccggac
     7261 cgggtgtcgg gacgccggac gccgcctcgc actgaggcgg aaggagatac atgccgtcat
     7321 agctcgcggc ggcgtagatc acgcccgagt acgacgccag gccccggata tcgccgtcca
     7381 cgggcgcgaa gtgccagccg agaacgcttg gctccgtcgg attgctcacg tccacgacgt
     7441 gcatcatcgt cccatagccg cccacgtaca gccgatcgcc gtcgaggtgc agggcttgcg
     7501 aggtgtcatc gaggagtacg tctgctgcca cgacggggtt ggccggcgtc gtcaggtcga
     7561 tggccaggac ctcaaagccg ctcgcttccg caaagacaaa cgagccgtcg aagacgaccg
     7621 cgccgatgga ctgcccaagg tcgagcgttc cgaccagctg cggggcgacc gggttcgtga
     7681 tgtcgaacaa ctggaaaccg tcctcgctgc cgacggcgag cctcgtgccg
//
LOCUS       DASVOD010000781         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_729029, whole genome shotgun sequence.
ACCESSION   DASVOD010000781 DASVOD010000000
VERSION     DASVOD010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729029"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="VF720_16720"
     CDS             complement(<1..1033)
                     /locus_tag="VF720_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX7881056.1"
                     /translation="MRLMTGAALLLLVTGSAGCTKKSGEAGGARAATTAPGKGPGGRG
                     GAVFPVEVVPVPGQPVEYAVSAVGSVDAFEIVQVTARVPGAVEQVHFREGDRVKEGAI
                     LVEIEPARYRLSVQEAEAAVARAEAALAEAESGLARREGANTTNAGLIPGEELDAWRT
                     RVATQKAEVASSQAALEMANRNLRDSQARAPFSGIIQTRDVQTGRYVTPGSLLTTLVR
                     RDPLLLRFQVPDGDAASLAPGMPARFSVRGAGDSHSAKIISVAESADPTSRMVAVTAE
                     VVNPVESLRPGAFAEVTVPVGAPRSAPVIPQTAVRPSERGFLAYVVSGGMARERVLAL
                     GLRTADGLVE"
     gene            complement(1036..2358)
                     /locus_tag="VF720_16725"
     CDS             complement(1036..2358)
                     /locus_tag="VF720_16725"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX7881057.1"
                     /translation="MQLRFSVPLSTLLLMMFVPDARALTLEEAIHRALERNERGLAAG
                     ETAEAARARVARARSFLLPELTATGDWTRRSHETTRTVDGVTSVLSTRDGLEGRLSLN
                     QVIFDARAFPLLREAKHSRDAAEFDAVEVRRQLAFDTAEIYLTVLAGEQVVRAAGERR
                     DLADQNTREVRVRFGAQLVSSNDVSRAELESAVAERELVEARGAVRVARLALSHLLDQ
                     PVTDSLEVPMTLLHGATEVPPMADVPDGTAPTDTAAVDRRPDVLASRSRVEALEAAAD
                     EPTARYLPQVNFNAQGWATNEAGFGGRGEDWNLGLGLTWPIFDGGDREAAKSERDALA
                     RAGALDLKNQERGVAVEIASARVAVESRQATLTQSDVAVQAARRNAAESAELYRRGLI
                     RALEAVDANVQLFEAEVEKTRAQFTLALAWLDLKAALGYDPLTETGIP"
     gene            2632..3849
                     /locus_tag="VF720_16730"
     CDS             2632..3849
                     /locus_tag="VF720_16730"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX7881058.1"
                     /translation="MPVESHRLPLLRDWHTHPLLYAAFADGVNLGEEAGASRESAIAW
                     IREHAAARGGSGWTIGYAWNSGRYPLAARDFDGLPPVVVMNLSLHGLIVNQSGRELLR
                     AQSPDIAHHLDDQEWIERNLLMVLNLFALDGSSPDRLRRFFDHLLTHHGIHAVDEMLL
                     VGPAEIASFDEAGLANRVRFWAAPGMFDALPPAMQRRVHGIKLFTDGALGTRSAALLA
                     PYPDGPGGTGMLLYSDDELAALLGRYGRHPLAIHAIGDRAIEQVVKAVEATRPAGPVR
                     MEHAQFITEPVALRARTLGMTLCMQPNFSDDAIHYRDRLPAGYPEKNNPFRMLIDSVG
                     FRPGEDLVFGSDGMPHGVSEALRQSLFPPHSGQVLTLDEFRAGYCLDDHDPGHIDVTI
                     DRAARSVSCRIVC"
BASE COUNT          599 a         1474 c         1320 g          630 t
ORIGIN      
        1 cttccacgag tccgtcggcc gtgcgcagtc ccagcgcgag gacgcgctcc ctggccatac
       61 cacccgacac cacgtacgcc aggaagccgc gctcgctcgg ccgcaccgcc gtctggggaa
      121 tgacgggcgc actccgcggc gcgcccaccg gcacggtcac ttcggcgaag gcgccgggtc
      181 gaagcgattc gacggggttc accacttcgg ccgtgaccgc caccatgcgc gaggtgggat
      241 ccgccgattc ggccaccgag atgatcttcg ccgagtgcga gtcgccggcg ccgcgcaccg
      301 agaatcgggc cggcatgccg ggcgcgaggc tggccgcgtc gccatccggc acctggaaac
      361 ggaggagcag cggatcgcgc cggaccagcg tggtaaggag gctccccggc gtcacgtagc
      421 ggccggtctg cacgtcgcgc gtctggatga tgccggaaaa gggggcgcgg gcctgcgagt
      481 cgcgcaggtt gcggttggcc atctcgagcg cggcctgcga ggaggccact tcggccttct
      541 gcgtggccac ccgcgtgcgc caggcatcga gttcttcgcc ggggatgagg ccggcgttgg
      601 tggtgttggc gccctcgcgc cgcgcgagcc ccgattccgc ctcggccagc gccgcctccg
      661 cgcgggccac cgcggcttcg gcttcctgca ccgacaggcg atagcgggcc ggctcgatct
      721 ccaccaggat ggcgccttcc ttcacgcgat cgccttcgcg gaagtgcacc tgctccacgg
      781 cgcccggcac gcgggcggtg acctgcacga tctcgaaggc atcgaccgaa ccgacggcgg
      841 agacggcgta ctcgaccggc tggccgggaa ccggcaccac ttccaccggg aagaccgcgc
      901 ctcccctgcc gcccggtccc ttgccgggcg cggtggtggc ggcccgggct ccgccggctt
      961 caccgctctt cttcgtgcag ccggcggacc ccgtcaccag gagaaggagg gccgcaccgg
     1021 tcatcaaacg catgatcatg gaatccccgt ttcggtgagc gggtcgtaac cgagcgccgc
     1081 tttcagatcg agccaggcga gggcgagcgt gaattgcgcc cgggtcttct ccacctcggc
     1141 ctcgaacaac tgtacgttgg cgtccacggc ctcgagcgcc cggatgagtc cccgccggta
     1201 gagctcggcc gactcggcgg cgttgcggcg ggccgcctgc accgccacgt cggattgcgt
     1261 gagcgtggcc tgccggctct ccaccgccac gcgcgccgag gcgatctcca cggccacgcc
     1321 gcgctcctgg ttcttgagat cgagcgcgcc ggcccgggcc aatgcatccc gctccgactt
     1381 ggccgcctcg cgatcgccgc cgtcgaaaat cggccaggtg agccccaggc cgaggttcca
     1441 atcctcgccc cgccccccga agccggcctc gttggtggcc cacccctgcg cgttgaagtt
     1501 cacctgcggc aggtagcggg ccgtcggctc gtcggccgcc gcctcgagcg cttccacccg
     1561 tgaccgcgag gccagcacgt cgggacgacg atcgaccgcg gccgtatccg tcggcgccgt
     1621 gccgtccggc acgtcggcca tcggcggcac ctcggtggcg ccgtggagca gcgtcatcgg
     1681 cacctccagg gagtcggtca ccggctgatc caacagatgc gagagcgcca gtcgcgccac
     1741 ccgtacggcc ccccgcgcct ccaccagttc gcgctcggcc acggccgatt cgagctcggc
     1801 gcgactcacg tcgttcgagc tcacgagctg cgccccgaag cgcacgcgca cctcgcgggt
     1861 gttctggtcg gccaggtcgc gacgctcccc cgccgcgcgc accacctgct cacccgccag
     1921 caccgtgagg tagatctcgg ccgtgtcgaa ggccagttgc cgccgtacct ccacggcatc
     1981 gaactccgcc gcatcccgtg agtgcttcgc ctcgcgaaga aggggaaaag cccgcgcatc
     2041 gaagatgacc tggttcagcg acagccgtcc ttccaggcca tccctcgtcg agaggacgga
     2101 ggtcacgccg tccaccgtcc gggtggtctc gtgcgaacgg cgcgtccaat caccggttgc
     2161 cgtcagttcg ggcaacagga atgaccgcgc ccgggccacg cgcgcccgcg ccgcctcggc
     2221 cgtttcgcca gccgccagcc cccgttcgtt ccgctcgagc gcgcgatgga tggcctcttc
     2281 gagcgtgagg gcacgggcat ccgggacgaa catcatcagg agcagcgtgg aaagggggac
     2341 agaaaaccgc aattgcatgc cggagaaacg tctcctgtaa cggggggaat cgacgaaccg
     2401 ggcggcggca atctcctgac ggaattggtc gccgtcgtga tcatgccgca aggagcggca
     2461 ccgatgcgcc ccctggaaca gaccgtgtgg cgacaaggtt ccgccaatct acgcgtaatc
     2521 tcaatgtttg cagatattta cgtgatcacg gaaccggtat cccgaatccg ttccacccta
     2581 tcggatttgt gtggtggaag tgtggcggcg gcggaaggtt aagctcccgt tatgcctgtt
     2641 gaatcgcacc gccttcccct tctccgcgac tggcacaccc atccactgct ctatgcggcc
     2701 ttcgccgacg gggtgaacct cggggaggag gccggcgcct cccgcgagtc ggccatcgcc
     2761 tggatccgtg agcacgcggc ggcacggggc ggctccgggt ggacgatcgg atacgcctgg
     2821 aacagcggcc gataccccct ggcggcccgc gatttcgatg gcctgccgcc cgtggtcgtg
     2881 atgaacctct cgctccatgg gctgatagtg aatcagtccg gacgggagct cctgcgcgct
     2941 caatcgcccg acatcgccca tcatctcgac gaccaggagt ggatcgagcg aaacctcctc
     3001 atggtgctca acctgtttgc actcgacggg tcttcgccgg atcggctgcg ccgcttcttc
     3061 gaccacctcc tcacccatca cggcatccac gcggtggacg agatgctcct cgtcggtccg
     3121 gcggagatcg cctcgttcga cgaggcgggc ctggccaacc gtgtccggtt ctgggccgcg
     3181 ccgggaatgt tcgacgcgct tccgccggcc atgcaacgcc gcgtgcatgg catcaagctc
     3241 ttcacggacg gcgcccttgg cacccgcagc gctgccctcc tcgcacccta tcccgatggg
     3301 ccggggggaa cggggatgct cctctactcc gacgacgaac tcgcggccct gctcgggcga
     3361 tacggccgcc accccctcgc catccacgcc atcggcgatc gggccatcga gcaggtggtg
     3421 aaggccgtgg aggcgacgcg ccccgcgggg ccggtccgca tggaacacgc ccagttcatc
     3481 accgaaccgg tggcccttcg ggccaggacg ctcggcatga ccctctgcat gcagcccaat
     3541 ttttccgacg acgcaattca ttacagggac cgcctgccgg cgggatatcc cgaaaagaac
     3601 aacccgttcc ggatgctgat cgacagcgtg gggttcaggc ccggcgagga tctcgttttc
     3661 ggttcggatg gcatgccgca cggggtgtcc gaggcgctgc gacagtcgct ctttccgccg
     3721 cattccggcc aggtgctcac gctggacgag ttccgggcgg gctactgcct cgacgatcac
     3781 gacccgggcc acatcgacgt gaccatcgac cgcgccgccc gatccgtctc ctgccgcatc
     3841 gtctgctaga atcccccgct ccgatgagca ccccgagtcc cgccatggcc accgcgaatc
     3901 ccgctcccga gcgcgcgttc cgtcgttccc tcgagtcggg gctggccgcg gcggcgcact
     3961 ggccgggcga tggcctggtc gtggcgctgt cgggaggctc cgattcggtg gcgctgctcc
     4021 acc
//
LOCUS       DASVOD010000782         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_625364, whole genome shotgun sequence.
ACCESSION   DASVOD010000782 DASVOD010000000
VERSION     DASVOD010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625364"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VF720_16735"
     CDS             <1..1020
                     /locus_tag="VF720_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX7881059.1"
                     /translation="NMATLYGAMLAGVHVVIMGAGIPREIPGVLDQLAEHRRATLRFD
                     VEHLSTGRQEFLRFDPARHWRAPAPLVRPQFLAIVSSNVLATTLARKSNGRVDGFVVE
                     GPTAGGHNAPPRRDPHLAPGASPTWGERDVVDLGKLGELGLPFWLAGGMGSPRALGKA
                     LAAGASGVQVGTLFAFCEESGFEPALKARILTALLRGDIDVRTDGQASPTGYPFKSLH
                     LRNDPEDVRREARTRVCDLGYLRTPFQSADGMIRYRCPAEPVEAWMKKGGDPAATVNR
                     RCLCNGLLAAVGQGQWRRTEGREPTVVTCGSDLEDLRALLAGRTGYTADDVIDWMLAD
                     SVVYR"
     gene            complement(1011..2912)
                     /locus_tag="VF720_16740"
     CDS             complement(1011..2912)
                     /locus_tag="VF720_16740"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX7881060.1"
                     /translation="MSSSSTISRPSREPQASAAPLHRRIPIASWLDPYARMLLVAALN
                     TPFLLAVLAFHIEGVSWTSPAAVYAVLAFLGYYVSFLLAGITVLFFVTWYSRRLFIGA
                     GTLLLALSLFYLFVNSIVHRIYHLHIDAFWIEYLVQNFSGIGFTPFMIGMAVAVLLGI
                     VALQAWIHRRVRRARNPGWIVTGLLVTCLLSLAGSQAVHVVAHETNDTRITSITPQLP
                     FYFPLRSHRNALKYGSLMSGIGEPSGPPATEGSRTLRYPRHEVSCVIPEGGRRPNILM
                     IVLESWRGDMLNATVSPAMSAFAARSSNFLNHLSSGNATPTGIFSLLYGLHSTYWLSV
                     KADNAAIDNPVLIDVLKANKYAFGIYADSHFSRHKIKDAMFRGIDVHESFSGSTADLK
                     DADLTDRLLAFIGAEQREGTPFFGFAFYKATHFSYYYPADDAPFQPARDVNPAIALSS
                     TDPTAFLNDYRNAIHYVDELVGRLLGRMDEMGVLENTIVIITSDHGEEFDDNHASYWG
                     HCGNFTEFQTRVPMIIHVPWKEPRVVMARTSHVDIPPTILQEGFGLGTEAGDYSNGCN
                     LFSPLENSRPMIVSSYVNHAIVVDDDVYVVNPTHLEKYKLSDIHAKAGSFTPATSKRA
                     IEEMSRFYR"
     gene            3073..3405
                     /locus_tag="VF720_16745"
     CDS             3073..3405
                     /locus_tag="VF720_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX7881061.1"
                     /translation="MESSFAIIAEPNRRAILGLLAGSERSVGELERRLKMPQTSVSKH
                     LRVLREAGFVESRIEAQRRVYRIRPEPLREVDAWLAQFRRTWTVHVDALERHLDRMEK
                     APAKGRKR"
     gene            3402..3947
                     /locus_tag="VF720_16750"
     CDS             3402..3947
                     /locus_tag="VF720_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX7881062.1"
                     /translation="MSNRANYTPGPAAGADVIKDGEKWTLVLVRELHHPPAKVWEALT
                     DPKQLAEWAPFDADRSLAAAGPVTLSTAGTPTPQASASTVTRAEAPRLLEYSWGGNDL
                     RWQLEPLTSGTRLTLWHKIDNRYIAWGAAGWHIAFDVLDHLLEGDPIGRMVGGDVMQF
                     DGWQRLTQEYTRHFTPRQEGS"
     gene            3944..4393
                     /locus_tag="VF720_16755"
     CDS             3944..4393
                     /locus_tag="VF720_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX7881063.1"
                     /translation="MKKSELEKAASAQIDRRIEELDDWRGRTLARVRKIFHQADPDVI
                     EEWKWMGTPIWSHDGIISTGEAYRSVVKLTFARGASLKDPKGLFNSSLEGKVRRAIDI
                     REGEKIDETALKALIREAVALNVAGKSKPKTTRPAAGKVAGRTSKKG"
     gene            complement(4434..>5086)
                     /locus_tag="VF720_16760"
     CDS             complement(4434..>5086)
                     /locus_tag="VF720_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX7881064.1"
                     /translation="REGNRVHLGISTGGHYMSEDGGETFTAANAGVGAGFTPDPYPEF
                     GQCVHKIAGHPDAPGRLYMQNHGGWAEWDGPGGRRPDIGVLRSDDYGRSWRSIAKGLP
                     SDFGFPIVVHPHDPDTVYVLPLEPMTRTCPGSAPAVWRSENGGDSWKKLSRGMPRKES
                     YFTVQRDGMDVDHLKSPALYFGTTTGQLWIGRDGGEEWSTLFDSLPPIHSVKVAVV"
BASE COUNT          918 a         1620 c         1713 g          835 t
ORIGIN      
        1 aacatggcca cactgtacgg ggcgatgctg gccggcgtgc acgtcgtgat catgggggcg
       61 ggcatcccgc gggagatccc gggcgtgctc gaccagctgg cggaacatcg gcgggcaaca
      121 ctgcgattcg atgtggaaca cctgtccacc ggtcgccagg agttcctgcg cttcgatccg
      181 gcgcggcact ggcgggcccc ggcgccgctc gtccgtccgc aattcctcgc catcgtgtcg
      241 tccaacgtgc tcgccacgac gctggccagg aagtccaacg gacgtgtcga cggcttcgtc
      301 gtggaaggac cgaccgccgg cggacacaat gcgcctcccc gccgcgaccc tcacctggcg
      361 cccggtgcct cgccgacatg gggtgagcgc gatgtcgtgg acctgggaaa actcggcgag
      421 ctcggccttc cattctggct ggccggtggc atgggatccc ccagggccct ggggaaagcg
      481 ctggcagcgg gcgcatccgg cgtgcaggtc ggcacgctct tcgccttctg cgaggaatcg
      541 ggattcgagc cggccctgaa ggcccgtatc ctgaccgcgc tcctccgggg cgatatcgac
      601 gtgcgtacgg atgggcaggc ctcccccacc ggctatccgt tcaagtcgtt gcacctgcgc
      661 aacgatccgg aagacgttcg ccgcgaggcg cgcacccgcg tctgcgacct cggctatctc
      721 cgcacgccct tccagtccgc cgacggaatg attcgatacc gctgccccgc cgagcccgtc
      781 gaagcctgga tgaagaaggg aggcgatccc gccgccaccg tcaaccggcg ctgtctctgc
      841 aacggcctcc tggcggccgt cgggcaggga cagtggcggc gtaccgaggg tcgcgagccg
      901 accgtggtga cgtgcggctc cgacctggaa gacctgcgcg cccttctcgc cggtcggacc
      961 ggctacacgg ccgacgacgt catcgactgg atgctcgccg actcggtcgt ctaccggtag
     1021 aagcggctca tttcctcgat tgcccgcttg ctggtggccg gcgtgaacga tccggccttc
     1081 gcgtggatgt cggagagctt gtacttctcg aggtgagtcg ggttgaccac gtagacatcg
     1141 tcgtcaacga cgatggcatg gttgacgtag ctgctcacga tcatcggacg cgaattttcc
     1201 agcggggaga agaggttgca cccgttgctg tagtcgcccg cttcggtgcc gaggccgaat
     1261 ccctcctgca ggatggtcgg cgggatgtcg acgtggctgg tccgggccat gacgacccgg
     1321 ggttccttcc agggaacatg gatgatcatc ggcacccggg tctggaactc cgtgaagttg
     1381 ccgcagtgcc cccagtagct ggcgtggttg tcgtcgaact cctcgccgtg atccgacgtg
     1441 atgatgacga tggtgttctc cagcactccc atctcgtcca tcctgccgag caggcgcccc
     1501 acgagttcgt cgacgtagtg gatggcattg cgatagtcgt tcagaaacgc ggtcggatcc
     1561 gtcgaggaga gggcgatggc ggggttcacg tccctggccg gctggaaagg tgcgtcgtcg
     1621 gccggatagt aatagctgaa gtgcgtggcc ttgtagaacg cgaacccgaa gaagggcgtc
     1681 ccttcccgtt gctcggcccc gatgaacgcg agcagccggt cggtcaggtc cgcgtccttc
     1741 aggtccgcgg tggaaccgct gaacgactcg tgcacgtcta ttccgcggaa catggcatcc
     1801 ttgatcttgt ggcgggaaaa gtgggaatcg gcatagattc cgaatgcgta cttgttggcc
     1861 ttgagcacgt cgatgaggac cgggttgtcg atggcggcat tgtcggcctt cacgctcaac
     1921 cagtaggtgg agtgaagacc gtacaggagc gagaagatgc cggtcggcgt tgcgttcccc
     1981 gaactcagat ggttgaggaa attcgaggac cttgccgcga aggccgacat ggcggggctc
     2041 accgtggcat tcagcatgtc cccacgccag ctctcgagca cgatcatcag gatgttcggg
     2101 cgccgccctc cctccgggat cacgcaggaa acctcgtgtc gcgggtaccg gagtgtccgc
     2161 gagccttccg ttgccggggg cccggagggc tcaccgatcc cggacatcag gctcccgtat
     2221 ttcagggcgt tgcgatgcga ccggagcgga aaatagaagg gcagctgcgg cgtgatgctc
     2281 gtgatgcggg tgtcgttcgt ttcgtgggcc acgacgtgaa ccgcctggct gcccgccagg
     2341 gacaggagac aggtaacgag aaggccggtc acgatccatc ccgggttccg cgcgcgccgg
     2401 acacgccggt ggatccacgc ctgcaatgcc acgatcccca ggaggacggc cacggccatg
     2461 ccgatcatga acggcgtgaa accgatcccg ctgaaattct gcacgaggta ctcgatccag
     2521 aaggcgtcga tgtgcaggtg gtagatccgg tgcacgatgc tgttcacgaa gagatagaac
     2581 agcgacagcg ccaggagcag cgtgcctgcg ccgatgaaca gccgcctcga ataccaggtg
     2641 acgaagaaca ggacggtaat gccggcaagc aggaaggaga cgtagtaccc gaggaaggca
     2701 agcacggcgt acacggcggc cgggctcgtc caggacactc cctcgatgtg gaaggcgagg
     2761 accgccagca ggaagggagt gttcagggcg gcaacaagaa gcatccgggc atacggatcg
     2821 agccacgagg cgatcgggat ccgtcgatgc agcggagccg cgctcgcctg aggttcccgt
     2881 gaggggcggg aaatcgtcga actggagctc atgtcgccgg cattgtaagt ccaatgccgt
     2941 ccactgcgaa cagaccttcc gcgcccttgg gcggaggatg tcaccgatcc ctgccccggg
     3001 acttccggcg gagtgaatca tcgccaataa tatgccttga caggtatata cctgaatgtg
     3061 catattaagg acatggaatc gtccttcgcc atcatcgccg agccgaaccg ccgggccata
     3121 ctcggcctcc tggccggctc ggaacgctcg gtcggggagc tcgagcgccg cctcaagatg
     3181 ccgcagacat cggtttccaa gcacctgcgc gtcctgcgcg aggccgggtt cgtggaatcg
     3241 cgcatcgagg cccagcgtcg cgtctaccgg atccgccccg agcccctcag ggaagtcgat
     3301 gcctggctcg cgcagttccg gcgcacctgg acggtccacg tcgatgcact cgaacgccat
     3361 ctcgaccgga tggagaaggc gccagcgaaa gggaggaagc gatgagcaat cgcgcgaact
     3421 acacgcccgg ccccgcggcc ggcgccgacg tcatcaagga cggggagaag tggacgctcg
     3481 tactcgtccg tgagctccat caccccccgg caaaggtgtg ggaggcactg accgatccga
     3541 aacaactggc cgaatgggcg ccattcgatg ccgaccggag cctggccgcg gcgggccccg
     3601 tgaccctctc gaccgccgga acgcccacgc cgcaggcgtc cgcaagcacc gtgacccggg
     3661 cggaagcgcc ccggttgctc gagtacagct gggggggcaa cgacctccgc tggcagctcg
     3721 agcccctgac ctccggcacg cgcctcaccc tatggcacaa aatcgacaac cgctacatcg
     3781 cctggggcgc cgcggggtgg cacattgcgt tcgacgtact cgatcacctg ctcgaggggg
     3841 atccgatcgg tcgcatggtc ggcggcgacg tcatgcagtt cgacggctgg cagcggttga
     3901 ctcaggaata cacccggcat ttcacccccc gacaggaggg atcatgaaga agtccgaact
     3961 cgaaaaggcg gcctcggcgc agatcgaccg gaggatcgag gagctggacg actggcgcgg
     4021 caggacgctc gccagggtcc ggaaaatatt tcaccaggcc gaccccgacg tcatcgagga
     4081 gtggaaatgg atgggaacac ccatctggtc ccatgacggg atcatctcga ccggggaggc
     4141 ctacaggagc gtggtcaagc tgacgttcgc caggggcgcg tcgctgaagg atccgaaggg
     4201 cctcttcaac tccagcctgg aggggaaagt ccgccgcgcg atcgacatcc gtgaaggaga
     4261 gaagatcgat gagacggcgc tgaaggcgct gatccgggag gccgtggccc tgaacgtggc
     4321 gggcaagagc aagccgaaga cgacgcggcc ggcggccggg aaagttgccg ggcggacgtc
     4381 aaagaaggga taggcaggtg tccgggggcc tgtcccggcg gcagcttctt tcctcagacc
     4441 accgccacct tcaccgagtg gatcggcggc agcgagtcga acagcgtcga ccactcctcg
     4501 cccccgtccc ggccgatcca gagctggccg gtcgtcgtgc cgaagtaaag cgcaggcgac
     4561 ttgaggtgat cgacgtccat gccgtccctc tgcaccgtga agtagctctc cttcctgggc
     4621 attcccctgg aaagcttttt ccaggagtcg ccgccgttct cgctgcgcca gacggccggg
     4681 gcgcttcccg ggcaggtgcg cgtcatcggt tcgagcggga gcacgtaaac ggtgtccggg
     4741 tcgtggggat gcacgacgat cgggaatccg aagtcggacg gcagtccctt cgcgatcgat
     4801 cgccagctcc tgccgtaatc atcgctccgg agcacgccga tgtcggggcg acgtcccccg
     4861 ggcccgtccc actcggccca tccgccgtga ttctgcatgt agaggcgccc cggggcgtcc
     4921 gggtggccgg cgatcttgtg cacgcactgg ccgaactcgg ggtagggatc gggcgtgaac
     4981 ccggcgccga cgccggcatt ggccgccgtg aaggtctcgc cgccatcctc gctcatgtag
     5041 tggccgccgg tcgagatccc gagatgcacg cggttccctt cgcgca
//
LOCUS       DASVOD010000783         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_782585, whole genome shotgun sequence.
ACCESSION   DASVOD010000783 DASVOD010000000
VERSION     DASVOD010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782585"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            451..2004
                     /locus_tag="VF720_16765"
     CDS             451..2004
                     /locus_tag="VF720_16765"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX7881065.1"
                     /translation="MSSSHHVRRSRLNVAEIDRVTAENPKSRVGLSPIGWAGLSLFLL
                     LLCLFLIILVDGRAVVHARDRSRPWSVALEAGDWPQVLTLAKAAVDKNARDAEAQNAY
                     GLALMETGKLDDAQNPLIAAEALSPEVPDYKVDLGDLYLKKGVVELAATRFREAVDLD
                     PSLVDVRWKLARALYTTQQYDETLAELTEITRLQPDNWDAYRLTADVAIGRKKYDEAI
                     QNLNLYTQVVRDSRALSKMAYAYMSLQPADTAGARTAAMRALEINPDDSQAHIALARI
                     SLIQKQNEEALVHYEKASAFFLPARDTYLMGRIYDAKKDLPKAGVAFRTAASIDSVNK
                     DYLGQAGSNAMLTQNWSDAADAYERLLQADPTNVSAVANLASALAQLGRMDEAGKLLE
                     AQIKTNESSPEVQLAMGDFQLKKEDKAAAKAAYQQALSLNPSKAVGSRASEALGYMLW
                     EEGDFAGAVPVLKNGLRYDECNIRSVLTLANSYVKLNQTPDAITLLRNAQSCPNSEQV
                     RQMLKALGG"
     gene            2029..>2261
                     /locus_tag="VF720_16770"
     CDS             2029..>2261
                     /locus_tag="VF720_16770"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX7881066.1"
                     /translation="MGVKTVMHGPVAVITPEGTLWGGDETSKLKETIDGLVAGGNTQL
                     VIDLGSVNHLNSTALGLLVSTHSGYAKRGGAVKL"
BASE COUNT          425 a          768 c          707 g          361 t
ORIGIN      
        1 ggggcgggcg cccccccggg aggcgccctg cacggcgcac ggaaacgatc gccccccgga
       61 cacgtgttct tccggaggcc gggggggcga tcgttttcgt gcgccacccg gaacgttccc
      121 gcgtgagagt ggtatgtccc ctttcccggg attttggatg gcgagcaagg taaccgtcat
      181 gtaaactccc gcttgacgcc acgggagaac tgcaatctcg ctcggttgtc cggctcgaat
      241 cgacccatcg gttccccacc gctcgggttg ctgcctccta tcgggccatc catcaagcag
      301 ttattgaagg tcgagtaggg ttcgggtatc gtggcgcgtc cctcaagggg gatggtcgcg
      361 gtcgccccgg cttccgtgca tccgtcacgg ctgatagtcc cgttttttcc acgcgttgcc
      421 ctcgcggatg ggtcggcgcg aaggagtccg atgtccagtt cgcatcacgt tcgccggtct
      481 cgcctgaatg tggcggagat cgatcgggta accgccgaga atccgaagtc acgggtgggc
      541 ctctccccga tcggctgggc cgggctttcc ctgttcctgc tgctcctctg cctcttcctg
      601 atcatcctcg tcgacggacg cgccgtggtg cacgcgcgcg accggagccg cccctggtcg
      661 gtcgccctcg aagcgggtga ctggccacaa gtgctcaccc tggccaaagc cgcggtggac
      721 aagaacgccc gtgacgccga agcccagaat gcctacggcc tggccctcat ggagaccggc
      781 aagctggatg acgcccagaa cccgctgatc gccgccgagg cgctctcgcc tgaagtgccg
      841 gactacaagg tcgacctggg tgacctctat ctcaagaagg gcgtcgtcga gctggccgcc
      901 acccgtttcc gtgaggccgt cgacctcgat ccctcgctgg tggatgtccg ctggaagctg
      961 gcccgggccc tctacaccac ccagcaatac gacgaaacgc tggccgagct gaccgaaatc
     1021 acccggctcc agcccgacaa ctgggatgcc taccggctta ccgccgacgt ggccatcggc
     1081 cgcaagaagt atgacgaagc catccagaac ctgaacctct acacccaggt ggtgcgcgac
     1141 tcgcgggcac tctcgaaaat ggcctacgcc tacatgtcgc tccagcccgc cgatacggcg
     1201 ggggcgcgca ccgcggccat gcgggccctc gagatcaacc cggacgacag ccaggcccac
     1261 atcgccctcg cgcggatcag cctcatccag aagcagaacg aagaggctct cgtccactac
     1321 gaaaaggcct ccgccttctt ccttcccgcc cgcgatacgt atctcatggg gcgcatctac
     1381 gacgccaaga aggacctgcc gaaggccggc gtggccttcc gcacggccgc ctcgatcgac
     1441 agcgtcaaca aggactacct gggccaggcc ggctcgaacg ccatgctcac gcagaattgg
     1501 agcgatgcgg ccgacgccta cgaacgactc ctccaggccg accccaccaa tgtttccgcc
     1561 gtggccaacc tggcctccgc gctggcccag ctcggcagga tggacgaggc cggaaagctg
     1621 ctcgaggcgc agatcaagac caacgaatcg agccccgaag ttcagctggc catgggcgac
     1681 ttccagctca agaaggaaga caaggctgcg gccaaggcgg cctaccagca ggccctctca
     1741 ttgaatccgt cgaaggcggt cggaagccgg gccagcgaag cgctgggata catgctctgg
     1801 gaggaaggcg atttcgccgg cgccgttccg gtgctcaaga acggcctgcg ttacgacgag
     1861 tgcaacatcc ggagcgtcct cacgctggcc aacagctacg tgaagctcaa ccagacgccc
     1921 gacgcgatca ccctgctccg caacgcgcag agctgtccga attccgaaca agtacgtcag
     1981 atgctgaagg cactgggggg ctgaggcccg ggtacccgag gaggatcgat gggagtcaag
     2041 acggtcatgc acggcccggt ggcggtcatc acgccggaag gaacgctctg gggcggtgac
     2101 gaaaccagca agctcaagga aacgatcgac ggcctcgtcg ccggcggaaa cacgcagctc
     2161 gtcattgacc tggggagcgt gaaccacctg aactcgacgg cgctggggct cctggtttcc
     2221 acgcactccg gctacgccaa gcggggcggt gcggtgaagc t
//
LOCUS       DASVOD010000784         6429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_677543, whole genome shotgun sequence.
ACCESSION   DASVOD010000784 DASVOD010000000
VERSION     DASVOD010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677543"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VF720_16775"
     CDS             <1..818
                     /locus_tag="VF720_16775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX7881067.1"
                     /translation="TAGHETDLIDRIQFDGGTGFSGWVARTQKPILFGSLKRSQPTHE
                     GVIKSFMAAPLVVAGRTIGVLTLGHSRENAFNRDDLRLLVMVGTQAAALLQKVILEAR
                     IREVSITDDATGLYTTGHFLSRVQDESARAGRFLQEFAVVRVSMDDFADFRTAVGPEA
                     ADRAIVEVASILRGLARGTDLVARTGPSEFALLLPVTNRDEAEAAARRLAHGIDNHVF
                     PRRKRLTASTAAVTFPEDGTDSQELLRAADNGLERARRLGRENFVEMPAVRAS"
     gene            831..2549
                     /locus_tag="VF720_16780"
     CDS             831..2549
                     /locus_tag="VF720_16780"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX7881068.1"
                     /translation="MTDERPAELPAGGDGAYSRPKILVVDDEESVLQVFVDLFAERDY
                     EVSTANNGEAAVAQLEAGTFDLLLTDLNLPGVDGLAVVAKAKMVDPEIVVLIITGQAT
                     ITTAIDALRHGAYDYIPKPFDLWAVDKVVTRGIQARRLKQENRQLLEYLTRANGELQR
                     HEEILKEKVNIATRQMSTLYEVGQRISESLNWSETMDVIVEKAVSLTGARSGLILLNQ
                     GNAGQLAGLAGVGLDAELVASFKIRTDEGLAGRVAKSRTAEIGENLTASGQEPLCRLG
                     AVSALAVPLLSKGELIGVINVLNKDGGPFQEDDQNVLTMFASQAAIAIVNSQLYSQAR
                     EIDRMKSEFVAVVSHELRTPLTAMKGSLELLSDRDYFTITDKQQELLGICMASVERLI
                     NQINDILDFSKIEGSKLPLNFETVEPVAIVRGVFEHLAPLARKKSIELAFEPQAHLPA
                     LHADGLRLAQVLTNLVGNALKFSPEKTTVEVAVGIDAGEMLFVVDDQGPGIADEDLPK
                     LFQKFQQVDSSHTRKVGGTGLGLFISRGIVEGHGGRIYVEAGKGGKGSRFCFRLPLDP
                     ALARAA"
     gene            2600..3421
                     /locus_tag="VF720_16785"
     CDS             2600..3421
                     /locus_tag="VF720_16785"
                     /inference="COORDINATES: protein motif:HMM:NF018406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 family protein"
                     /protein_id="HEX7881069.1"
                     /translation="MSTPESPPADGGPIQNFVRSFYSRRFYREAGLFPMPAALVNWLL
                     LALVLGLVTGIQLSGAVGRGLVDWNARLDAGTVPAVRINHGHLEVRGEQPFVQMEPSG
                     LLVVDTTGTWTALPDSVPAGLLVTRDRVVFKPSSGVSRTYELAGQHFDFWLDSAGLRR
                     VRGVAIPIVLLVMTPMAFFYFALINLSLVLILVGAAMAADRIFGNRVGLPFRTLLKLG
                     LFVVTPVAIGFKFLGLVAPALSEALLPFYPAVTAFLLLGAIRQSVAATTPETGAD"
     gene            3425..4912
                     /locus_tag="VF720_16790"
     CDS             3425..4912
                     /locus_tag="VF720_16790"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX7881070.1"
                     /translation="MDPLRRAATSGAAFSLVSIVGMRAFGFLTTFFLTHVLNQDDFGR
                     YALILSYNAVGVLVAGLGVTIIAPKLIAETVPPDRPPGPDTQAILRSFLTIAGVAALV
                     VGLIVTLCATAIRGALAANGASWSMLGVLGLTVAATSLSAAIAAMLQGLHRTRELAIF
                     NTLIAGLYLLAAIPAARAFGVTGAVATLAACHGVVMLAALAWLPPGARQPLLSAKTRE
                     VLARGVNLGWPLLVSSLALAMSGSWLRTWLGKSVSVAEVGRFQVADTLNQAILFVPLA
                     LATPLFPLASTMGRATPEERARVFGPAFAFIALLSLPATLVVGWGSRFWIGLFNAEYH
                     AAWPVVYLLTAGYFLTSLTTITGALLAGFGLMMDSLKITVFWSALLALSAMLLAPRYG
                     AMGVAAGYVVSAIIQTAVMAIYYRRRLGIRLLAAPRVLVLFPILFALGWWLLGNVSPL
                     SGLLLAIPASGLVLLTSLDLTRRAISHLLRWIRPTTTVDAGAGPA"
     gene            4909..5802
                     /locus_tag="VF720_16795"
     CDS             4909..5802
                     /locus_tag="VF720_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881071.1"
                     /translation="MIRTPVDRSRRWLVGCHRGMGDLLYVSSALTRLRNELPPGIGLD
                     LAAGRGLWADAVRHLPAVDRILPTDSWDDITRLARDGGYERVLVARVTEWMPWRRYFG
                     WTLRAQLEGKHLVDVLAATLGVRLDPADRHPLYVSDPSDPEDDPTPARELGRYAVVAL
                     HGYTHHGHNESAWRLLLPPLLDDATKRAHRLVIVGPPGEPIVAAPGLVDRRGESIRRV
                     AALVERAAVVVSVLNGITVLADALERPVVLLALGRDPLSVVGPLRPPLAVFGAPGSKG
                     ASAVNVSAFVDRVGRALAEES"
     gene            5804..>6429
                     /locus_tag="VF720_16800"
     CDS             5804..>6429
                     /locus_tag="VF720_16800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX7881072.1"
                     /translation="MAVVLFVNSTAMHGGAEEALLHLVRAAVQAGHRPVLATPGEGWL
                     PAAARESGAITEVVPRLPEAMATDSALAQFGPWLPAATGLARIARRHGAAIVHSNTPR
                     TAYHGGLAARLAGARAITHCYDILGLPYASTPKAWLLDRLSDWTLTVSNAVATAILAH
                     APRMAGRMSTLYHGFDAARIAATPPADLTALFGVPRNAGVVVHAAAMTP"
BASE COUNT          900 a         2309 c         2143 g         1077 t
ORIGIN      
        1 ccaccgccgg tcacgagacc gacctgatcg accgcatcca gttcgacggc ggcaccggct
       61 tctccggctg ggtggcccgt acgcagaagc cgatcctctt cggctcgctc aagcgcagcc
      121 agcccacgca cgagggggtg atcaagtcgt tcatggcagc gccgctcgtg gtggccggcc
      181 ggacgatcgg cgtcctcacc ctcggtcatt cgcgggaaaa cgccttcaat cgcgacgacc
      241 tccgcctcct ggtcatggtg gggacgcagg ccgcggcgct gctccagaag gtgatcctcg
      301 aggcccggat ccgcgaggtg tcgatcaccg acgatgccac cggactctac acgaccgggc
      361 acttcctctc gcgcgtgcag gacgagtcgg cccgggccgg acgcttcctc caggagttcg
      421 cggtcgtccg ggtgtcgatg gatgacttcg ccgatttccg taccgcagtg gggccggagg
      481 ccgccgaccg tgccatcgtc gaagtggcct ccatcctgcg cggcctggcc cgcggcaccg
      541 acctggtggc ccggaccggc ccctccgaat tcgcgctcct cctgccggtg accaaccggg
      601 acgaggccga ggcggccgcc cgtcggctgg cccacgggat cgacaaccac gtcttcccgc
      661 gccggaagcg cctgaccgcc tccacggccg cggtcacctt ccccgaggac ggcaccgact
      721 ctcaagaact gctgcgcgcc gccgataacg gtctggaacg ggcgcgtcgc ctgggccggg
      781 aaaattttgt cgagatgccg gccgtgcgcg cctcctgacg ggagtgttga gtgaccgacg
      841 aacgtcctgc tgaactgccc gccggcggcg atggcgccta cagccgcccg aagatcctgg
      901 tcgtcgacga cgaggagagt gtcctccagg tcttcgtcga cctgttcgcc gagcgcgact
      961 acgaagtgtc caccgccaac aacggcgagg ccgccgtggc ccagctcgag gcgggcacgt
     1021 tcgacctcct cctgaccgac ctcaatcttc ccggcgtgga cggccttgcc gtcgtggcca
     1081 aggccaagat ggtcgacccg gagatcgtgg tcctcatcat taccggacag gccacgatca
     1141 ccaccgccat cgacgcgctg cgtcacgggg cctacgacta catccccaag cccttcgacc
     1201 tgtgggccgt cgacaaggtg gtcacgcgcg gcatccaggc caggcggctc aagcaggaaa
     1261 accgccagct cctcgagtac ctgacccggg ccaacgggga gctgcagcgg cacgaggaaa
     1321 tcctcaagga aaaggtcaac atcgccaccc ggcagatgag cacgctctac gaagtggggc
     1381 agcgcatctc cgagagcctg aactggagcg agacgatgga cgtcatcgtc gagaaggcgg
     1441 tgagtctcac cggtgcccgt tccggcctca tcctcctgaa ccaggggaat gccgggcagc
     1501 tggccggcct ggccggcgtg ggactcgacg ccgagctggt ggcctccttc aagatccgca
     1561 cggacgaagg gctggccggc cgcgtggcca agagccgtac ggccgagatc ggcgaaaacc
     1621 tcacggcctc gggccaggag ccgctctgcc ggctgggcgc cgtttcggcc ctggccgtgc
     1681 cgctcctttc caagggggag ctaatcggcg tcatcaacgt gctcaacaag gatggcgggc
     1741 cgttccagga agacgaccag aacgtcctca ccatgttcgc cagccaggcg gccatcgcca
     1801 tcgtgaactc gcagctttac agccaggcgc gcgagatcga ccggatgaag tccgagttcg
     1861 tggccgtggt gtcacacgag ctgcgcacgc cgctgaccgc catgaagggg tcgctcgagc
     1921 tcctgtcgga cagggattac ttcacgatca ccgacaagca gcaggaactc ctcggcatct
     1981 gcatggccag cgtcgagcgc ctcatcaacc agatcaacga catcctcgac ttctccaaga
     2041 tcgaggggag caagctgcca ctcaacttcg agacggtcga accggtggcg atcgtccgag
     2101 gggtgttcga acacctggcc ccgctggcca ggaagaagtc gatcgagctg gcgttcgagc
     2161 cacaggccca cctgccggcg ctccacgccg acggacttcg cctggcgcag gtcctgacca
     2221 acctggtcgg gaacgcgctc aagttctcac ccgagaagac cacggtggag gtggccgtgg
     2281 gcatcgacgc cggcgagatg ctcttcgtcg tcgacgacca gggtcccggc atcgccgacg
     2341 aggatctccc gaagctcttc cagaaattcc agcaggtcga ctcgtcccat acccgcaagg
     2401 tgggggggac gggcctcggg ctcttcatct cgcgcggcat cgtggaaggg cacggcggcc
     2461 ggatctacgt ggaagcgggc aagggaggca agggaagccg cttctgcttc cggctgccgc
     2521 tcgatcccgc cctggcgcgc gccgcctgat ttcccgcggg gactgaccga cggacgggcc
     2581 ggctgatatt ctccggcgcg tgtccacccc cgagagtcct cccgccgacg gcgggccgat
     2641 ccagaatttc gtccgcagct tctacagcag acgcttctat cgcgaggccg gcctctttcc
     2701 gatgccggcg gcgctggtca actggctcct cctggccctg gtgctcgggc tcgtcaccgg
     2761 catccagctc tccggcgcgg tcggccgggg gctggtcgac tggaatgccc gcctcgacgc
     2821 gggaacggtg ccggccgtgc gcatcaacca tgggcacctc gaagtgcgcg gggagcagcc
     2881 gttcgtccag atggagccga gcgggctcct ggtggtcgac acgaccggca cctggacggc
     2941 gcttcccgac agcgtgccgg ccggtctcct cgtgacgcgc gatcgcgtgg tgttcaagcc
     3001 gtcgagcggc gtatcgcgca cgtacgaact ggccggccag cacttcgact tctggctcga
     3061 ctcggccggg ctccggcgag tgcgcggcgt ggcgattccc atcgtgctcc tggtcatgac
     3121 ccccatggcc ttcttctact tcgcgctgat caatctgtcg ctggtgctga tcctggtcgg
     3181 cgccgcgatg gccgccgacc ggatcttcgg caaccgcgtt gggctgcctt tccggaccct
     3241 cctgaagctg ggcctcttcg tggtgacgcc ggtggccatc gggttcaagt tcctcggcct
     3301 cgtggcgccc gcattgtcgg aggcgctgct gccgttctat ccggcggtca ctgcgttcct
     3361 cctcctgggc gccatccgtc agagcgtggc cgccacgact ccggaaaccg gcgcggactg
     3421 acgcgtggac ccgctgcggc gtgccgcaac ctcgggagcg gccttctcgc tcgtctcgat
     3481 cgtcgggatg cgcgccttcg gattcctgac gaccttcttc ctgacgcacg tcctgaacca
     3541 ggacgacttc gggcgatatg cgctcatcct gagctacaac gccgtcggcg tgctggtggc
     3601 cggcctcggc gtgacgatca tcgcgcccaa gctgatcgcc gaaacggtgc cgcccgaccg
     3661 ccctcccggc cccgacacgc aggccatcct gcgatcgttc ctgacgatcg cgggcgtggc
     3721 ggctctggtg gtcggcctca tcgtgacgct ctgcgccacg gccatccggg gcgcgctcgc
     3781 ggccaacggg gcctcgtggt cgatgctcgg cgtgctcggc ctcaccgtgg cggccacctc
     3841 cctgtcggcg gccatcgcgg cgatgctgca ggggctccac cgcacgcgcg agctggcgat
     3901 cttcaacacg ttgatcgccg gactctacct gctggcggcg ataccggcgg cccgggcatt
     3961 cggcgtgacc ggcgcggtgg ccaccctggc ggcctgccac ggggtcgtga tgctggcggc
     4021 gctggcctgg ttgccgccgg gcgcccgtca gccgctcctg tcggcgaaga cgcgtgaggt
     4081 gctggcccgc ggagtcaacc tcggctggcc gctcctcgtc tccagtctgg cgctggccat
     4141 gtccggttcg tggctccgca cctggctggg aaagagcgtg agcgtggcgg aagtcggccg
     4201 cttccaggtg gccgacacgt taaaccaggc catcctcttc gtgccgctgg cgctggccac
     4261 gccgctcttt ccgctggcct cgacgatggg tagggccacg ccggaggagc gggcgcgcgt
     4321 gttcggtccg gccttcgcct tcatcgcgct tctctccctt ccggcgaccc tggtcgtcgg
     4381 atggggatcg cgcttctgga tcggcctctt caacgccgag taccatgcgg cctggcccgt
     4441 cgtgtacctc ctcacggccg gctatttcct gacgagcctc accacgatca ccggcgccct
     4501 gctcgccgga ttcgggttga tgatggacag cctcaagatc acggtcttct ggtcggcgct
     4561 gctggcgctg agcgcgatgc tgctggcgcc aaggtacggc gccatgggcg tggccgccgg
     4621 ctacgtcgtt tcggcgatca tccagacggc ggtgatggcg atctactacc gacggcggct
     4681 cggcattcgc ctgctggccg cgccgcgcgt gctggtgctc tttccgatcc tcttcgcgct
     4741 tggctggtgg ctgctcggga acgtatcgcc gctctcgggt ctcctcctgg cgattccggc
     4801 ctcgggcctc gtgctgctca cctcactcga cctgacgcgc cgcgccatca gccatctcct
     4861 gcgctggatc cggccgacga cgaccgttga cgccggcgcc gggcccgcgt gattcggact
     4921 ccggtcgatc gatcccgtcg ctggctggtc ggctgccacc ggggaatggg cgacctcctc
     4981 tacgtgtcgt cggccctcac ccgcttgcgg aacgaactgc cgcccgggat cgggctcgac
     5041 ctcgccgccg gccgcggcct ctgggccgac gcggtacgtc acctgccggc cgtggatcgc
     5101 atcctcccga ccgattcgtg ggacgacatc acgaggttgg ctcgtgacgg cggctacgag
     5161 cgcgtcctcg tggcccgcgt caccgagtgg atgccgtggc gacgctactt cggctggacc
     5221 ttgcgggcgc aactcgaagg gaagcatctc gtggatgtcc tcgcggccac gcttggcgtg
     5281 cggctggatc ccgccgaccg gcaccccctg tacgtgagcg atccttccga tccggaagac
     5341 gacccgacgc cggcccgcga gctcggccgt tatgcggtgg tggcgctcca cggctacacg
     5401 caccacggtc acaacgagtc agcgtggcga ctgcttctgc cgccgctgct ggacgatgcc
     5461 acgaagcgcg ctcatcgact ggtgatcgtc ggcccgccgg gcgagccgat cgtggccgcg
     5521 cccgggctcg tcgaccgtcg tggcgaatcc atccggcgcg tggcggcgct ggtggagcgg
     5581 gccgccgtgg tcgtctcggt gctgaacggg atcaccgtcc tggcggatgc gctggagcgc
     5641 cccgtggtcc tgctcgccct cggacgcgat ccgctttcgg tggtgggtcc actccggccc
     5701 cccttggcgg tattcggcgc gccggggtcc aagggtgcgt cggcggtgaa cgtgtcggcg
     5761 ttcgtcgacc gcgtcggccg cgcactggcg gaggagagct gacgtggccg tcgtcctctt
     5821 cgtcaattcc acggccatgc acggcggggc cgaagaggcg ctgcttcacc tggtgcgggc
     5881 cgctgtgcag gccgggcatc gcccggtcct cgccacaccc ggggagggat ggctcccggc
     5941 cgccgcccgg gagtcggggg ccatcaccga ggtcgtgccg cgtcttccgg aagcgatggc
     6001 caccgacagc gccctggccc agttcggacc gtggctcccc gcggccacgg gcctggcgcg
     6061 catcgcccgt cgtcacggtg ccgcgatcgt tcactccaac acgccgcgca ccgcctacca
     6121 cgggggattg gccgcgcgac tcgcgggtgc ccgcgcgatc acccactgct acgacatcct
     6181 gggcctgccg tacgcatcga cgccgaaggc ctggctcctc gatcggctca gcgattggac
     6241 cctcaccgta tcgaacgcgg tggccaccgc catccttgcc cacgcgcccc ggatggccgg
     6301 ccggatgtcc acgctgtacc acgggttcga cgccgcgcgc atcgcggcca ccccgcccgc
     6361 cgacctcacg gcactcttcg gggtgcctcg aaacgcgggc gtcgtcgttc atgccgcggc
     6421 catgacccc
//
LOCUS       DASVOD010000785         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_625598, whole genome shotgun sequence.
ACCESSION   DASVOD010000785 DASVOD010000000
VERSION     DASVOD010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625598"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1799)
                     /locus_tag="VF720_16805"
     CDS             complement(<1..1799)
                     /locus_tag="VF720_16805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX7881073.1"
                     /translation="MSHPHAALPANRSDRAPLWVPLVVLAIALLVRILYRQQAGHVLL
                     FNEFTGDSAQYLELAGRLKNEGLMAPKGEAWFQAPFYPFFLFLAGASPTHLELARWLQ
                     FAAGATGALLAWDTARRLAGSTAGLMAGLIAAVYGPRVFFDGEMLSIGWSLFFLQLMA
                     WLLVLRHESRDRPPSPALDLPLGVTGGLAALAQPNTLVASVGVIGWLFVAGRRTPHAR
                     RGAILTALGVLLVILPVTLRNKAESRDWVLISANGGINFFIGNNPEADGTFHLPPREP
                     LLNDPSGLVTASREAAARALGHPASPSATSGYWMNRGLAWWGSAPLDAVSLTARKVLY
                     LLNNIEIPNHYDFAWFTQRVPILRLLPGFWLLLPLAAWGLARIGREPIGRLFALLLGA
                     LLLSVAFFFVTDRYRLPIVAFLFPVAGIGAADLWSIARGERPGARLPAILVVSAFLLL
                     AAFPLVDTRPSMAHMHNLVGTLHYKRGEASAARREFEAALAISPNLAEPANNLGRLAL
                     NDRDDAGAERWFRKALELDPRRAEAWFNLEELERSRRRYREALALIDGMERQVPGVTE
                     SFGGALAYRRGLSFQALGDTARAGASLREAIRL"
     gene            1876..>2609
                     /locus_tag="VF720_16810"
     CDS             1876..>2609
                     /locus_tag="VF720_16810"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEX7881074.1"
                     /translation="MIDFQRQLLQLLESGRRVAVATIVEARGSTPQREGGRIIVAEDG
                     ATWFTIGGGAFEALVIDDAKECLRSGRSDLKSYSLHDSGDEALGMACGGNAMVFIDVP
                     PIAPPLLIFGAGHVGRALARLAVGFGFRVSVIDDRPDMLDTAAFPAGTTLIATDRSFR
                     ENVPPIDAATSIVLVTRCHDTDEAAFEGVAHAPWSYLGMIGSRRKVKVVFDRLVERGM
                     ARDILDRIHAPIGVPIGSHLPEEVAVS"
BASE COUNT          456 a          825 c          966 g          362 t
ORIGIN      
        1 ttcagtcgga tggcctctcg cagcgacgcc ccggcccggg cggtgtcgcc cagcgcctgg
       61 aacgagaggc cgcgccggta ggccagggcg ccgccgaacg actcggtcac gccgggtacc
      121 tggcgctcca tgccgtcgat cagggccagc gcctcccggt atcggcggcg cgatcgctcg
      181 agttcctcca ggttgaacca ggcctcggcg cggcggggat caagctccag cgccttccgg
      241 aaccagcgtt cggctccggc atcgtcgcga tcgttgagcg ccagccgccc caggttgttg
      301 gccggctcgg cgaggttcgg cgagatggcg agcgccgcct cgaactcgcg ccgcgccgcg
      361 cttgcctcgc cccgcttgta gtgaagcgtg ccgaccaggt tgtgcatgtg ggccattgac
      421 ggcctggtgt cgaccagcgg aaaagcggcc agcaggagaa atgccgacac gacgaggatt
      481 gcggggaggc gggcgccggg tcgttccccg cgggcgatcg accagagatc cgccgccccg
      541 atgccggcaa cggggaagag gaatgccacg atcggcaggc gatatcgatc ggtcacgaag
      601 aagaaggcca ccgacagaag gagcgcgccg agcagcagcg cgaagaggcg gccgatcggc
      661 tcccgcccga tccgggccag tccccaggcc gccagcggca ggaggagcca gaagccgggc
      721 aggagacgga ggattggaac ccgctgcgtg aaccaggcaa agtcatagtg attcgggatc
      781 tcgatgttgt tcaggagata gaggaccttc cgggccgtga gtgatacggc atcgagcggc
      841 gccgagcccc accaggcgag tccgcgattc atccagtagc ctgacgtggc cgacggactg
      901 gcgggatgcc cgagcgcgcg cgcggcggcc tcccgcgacg cggtgacgag gcccgaggga
      961 tcgttgagaa gcggttcccg gggcgggaga tggaaggtgc cgtcggcttc aggattgttg
     1021 ccgatgaaga aattgatgcc gccgttggcg gagatcagca cccaatccct tgactccgcc
     1081 ttgttgcgga gggtgacggg gaggatgacg agcaggacac ccagagccgt caggatggca
     1141 ccgcgccggg cgtgcggcgt gcggcggccg gcaacgaaaa gccaaccgat cacgccaacc
     1201 gaggccacca gcgtattcgg ctgcgccaac gccgccagtc cgcccgtgac gccgagcggg
     1261 aggtcgagcg cgggggaggg cggccgatcg cgcgactcgt gacgcaggac gaggagccat
     1321 gccatcagct ggaggaaaaa gagggaccag ccgatcgaga gcatctcgcc gtcgaagaag
     1381 acgcggggtc cgtagaccgc cgcgatgaga ccggccatga ggccggcggt cgatcccgca
     1441 aggcgccgcg ccgtgtccca cgcgaggagc gcgcccgtgg cgccggcggc aaactggagc
     1501 cagcgggcca gttcaagatg ggtcggcgat gcccccgcga ggaagaggaa gaaagggtag
     1561 aacggcgcct ggaaccaggc ctctcccttt ggcgccatga gcccttcgtt cttcagtcgc
     1621 ccggccagtt cgaggtactg ggcggagtca ccggtgaatt cgttgaagag gaggacgtgc
     1681 cccgcctgct gccggtagag gatgcgcacg aggagcgcga ttgcgaggac gacgagcggc
     1741 acccagagtg gcgcccggtc ggaccggttc gcggggaggg cggcgtgagg atggctcatg
     1801 ggaggccgaa agtatagaaa cgaccgcgga agccggtcaa cggagaggcg cggcgtgcgg
     1861 taaactcgtc gacccatgat cgacttccag cgccagctgc tccaactgct cgagtcgggc
     1921 cgccgtgtgg cggtggccac catcgtcgag gcacgcggct ccacgcccca gcgcgaaggg
     1981 gggcgcatta tcgtggccga ggacggcgcc acctggttca ccatcggcgg cggagcgttc
     2041 gaggcgctgg tcatcgacga tgcgaaggag tgcctcaggt ccggccgctc cgacctgaag
     2101 agctactcgc tgcacgactc gggggatgag gcgctgggga tggcctgcgg agggaacgcg
     2161 atggtcttca tcgacgtgcc gccgatcgcg ccgccgctcc tcatcttcgg cgccggccac
     2221 gttgggcgcg cgctggcccg gctggcggtg ggctttggct tccgggtgtc ggtcatcgac
     2281 gatcgtccgg acatgctcga caccgcggcc tttccggcgg gcacgacgct gatcgccacc
     2341 gatcggagct tccgcgagaa cgtgccgccg atcgatgccg ccacgtcgat cgtgctcgtc
     2401 acccgatgcc atgacacgga cgaggcggcg ttcgaaggtg tggcccatgc gccctggagc
     2461 tatctcggca tgatcgggag ccggcgaaag gtgaaggtgg tcttcgatcg actggtcgaa
     2521 cgggggatgg cgcgcgacat cctcgaccgg atccacgcgc cgatcggcgt tccgatcgga
     2581 tcgcatcttc ccgaggaagt tgccgtgtc
//
LOCUS       DASVOD010000786         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_729722, whole genome shotgun sequence.
ACCESSION   DASVOD010000786 DASVOD010000000
VERSION     DASVOD010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729722"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VF720_16815"
     CDS             complement(<1..600)
                     /locus_tag="VF720_16815"
                     /inference="COORDINATES: protein motif:HMM:NF013673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEX7881075.1"
                     /translation="MLSDPLFEPSAGISRRKFLARSLTAGVGLAAANLTWETLAAREA
                     GGATAADLPELTKFLSRDQVAELIRTALTAGGEFAEVYAEYTINTGIRLDEKKVKSVD
                     YNILSGVGIRVIDGDQVGYAYADTYDMAELKEAARVAAAIARAGGGLKPKPFQVSTAK
                     PPFILKTPLPLNLAEEAKLELCRRVDGAARGHDAKISQVT"
     gene            761..1819
                     /locus_tag="VF720_16820"
     CDS             761..1819
                     /locus_tag="VF720_16820"
                     /inference="COORDINATES: protein motif:HMM:NF022720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HEX7881076.1"
                     /translation="MSFVNCRFIPVSVARAAAFGLALTAFVSAAAPALGADPIPPGPW
                     MVDLRLGALLSQSSYSNNWAKGDQGSFSWVANMDFDAERQFSVKFNWDNYLRLAYGQT
                     GQQVADPDHPGKNSWEPPNKTTDQILAESTGRFTLGGFVDPFIGIRGESQFEDESNSY
                     GTLNFNPVRLTETAGLARVFKKSAYTEVASRLGLGARQTFGKEFINDGADKRSFSTND
                     GGVEWFSTAKLPLVDSTRVVYNGRLLVFAPVFYSQSDELERFDEAALALDPNHEEVSD
                     FWRVPDVDWQNEFVARISKVVNVNLLVHMVYDKYDANTNVAGERPVDELAPLVNGAVR
                     KSAQWRQTLALGLTYQIF"
     gene            1829..2227
                     /gene="mscL"
                     /locus_tag="VF720_16825"
     CDS             1829..2227
                     /gene="mscL"
                     /locus_tag="VF720_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020193520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX7881077.1"
                     /translation="MLKEFKEFALKGNVLDLAVGVILGAAFGALVSSLVNNVMMPPIG
                     VVTGGVDFSRLGIMLKPAEGDKPATMLSYGVFINSVITFLITAFAVFLLVKNVNRFRK
                     QEAAAPAAPPEPSAEVKLLTEIRDLLRSRG"
BASE COUNT          394 a          782 c          751 g          416 t
ORIGIN      
        1 ggtcacctgg gagattttcg cgtcatgccc gcgcgccgcg ccgtccaccc gccggcagag
       61 ctcaagcttg gcctcctcgg ccaggttgag cggaagcggc gtcttgagga tgaacggcgg
      121 ctttgccgtc gacacctgga agggcttcgg cttcaagccg ccgccggccc ttgcgatggc
      181 cgccgccacg cgggcggcct ccttcagctc ggccatgtcg tacgtgtcgg cgtaggcgta
      241 gccgacctgg tcaccgtcga tcacccggat gccgaccccg gagaggatgt tgtaatcgac
      301 cgacttgacc tttttctcgt cgaggcggat tcccgtgttg atcgtgtact cggcatacac
      361 ctccgcgaac tcgccgccgg ccgtgagggc cgtcctgatc agttcggcca cctggtcgcg
      421 cgaaaggaat ttcgtgagct cgggaaggtc ggcggccgtg gccccgccgg cctctcgggc
      481 ggccaaggtt tcccaggtga gattggccgc ggcgagaccc acgccggcgg tcaggctgcg
      541 cgcgagaaat ttccgccgcg agatgccggc ggacggttcg aaaaggggat ccgacaacat
      601 gacgcctccg gaagccgcag ggtgatgggc gttggcacgc ggggaccgat tacgtggagc
      661 gcggagaata gacccggggc cgcgcctcga caacacccct caaatcgcgc accatcgccc
      721 gccatcgccc ctttccatta cccgcaggag gaccgtcgtg atgtcgttcg tcaattgccg
      781 tttcatcccg gtgtccgtgg cacgcgccgc cgccttcggc ctcgctctca cggccttcgt
      841 gtcagcggcc gctccggcac tgggcgccga ccccattcct cccggcccgt ggatggtgga
      901 cctcaggctc ggtgccctcc tctcgcagag ttcgtacagc aacaactggg cgaagggaga
      961 tcaggggtcg ttcagctggg tagccaacat ggacttcgac gccgagcgcc agttcagcgt
     1021 taaatttaat tgggacaatt acttgcggtt ggcatacggc cagaccgggc agcaggtcgc
     1081 cgaccccgat cacccgggca agaacagctg ggagccaccg aacaagacca ccgaccagat
     1141 cctcgcggag tcgaccgggc gcttcacgct gggcggcttc gtcgatccct tcatcggcat
     1201 ccgcggggaa tcgcagttcg aggatgagtc gaacagctac ggcacgctca acttcaaccc
     1261 ggtgcggctc accgaaacgg cgggccttgc ccgcgtcttc aagaagtccg cgtacaccga
     1321 agtggcttcg cgcctgggcc tcggcgcccg ccagacgttc gggaaggaat tcatcaacga
     1381 cggtgccgac aagcgcagtt tttccaccaa cgacggtggc gtggagtggt tttccaccgc
     1441 caagctgccg ctggtcgact ccacccgggt ggtttacaac gggcggctcc ttgtcttcgc
     1501 gccggtcttc tacagccagt cggatgaact ggagcggttc gacgaggcgg ccctggccct
     1561 cgacccgaac cacgaggagg tgtcggactt ctggcgcgtt ccggacgtcg actggcagaa
     1621 tgagttcgtg gcgcgcattt ccaaggtcgt caatgtcaat cttctcgtgc acatggtgta
     1681 cgacaagtac gacgccaaca ccaacgtggc cggcgagcga ccggtcgatg agctggcgcc
     1741 gctggtcaac ggggccgtcc gcaagagcgc gcagtggcgc cagacgctgg cgctgggtct
     1801 cacctaccag atcttctgag gaggagccat gctgaaggaa ttcaaggaat tcgcactgaa
     1861 aggaaacgtg ctcgacctgg ccgtcggcgt gatcctcggt gccgcattcg gcgccctggt
     1921 gagctccctg gtgaacaacg tgatgatgcc gccgatcggt gtcgtgacgg ggggcgtcga
     1981 cttctcgcgg ctcgggatca tgttgaagcc ggccgaaggg gacaagccgg ccaccatgct
     2041 ctcctatggc gtcttcatca attcggtgat caccttcctg atcaccgcgt tcgccgtctt
     2101 cctcctggtg aagaacgtca acagattccg caaacaggag gcggccgccc cggcggcgcc
     2161 tcccgagccg tcggccgagg tgaaactcct taccgagatc cgcgacctcc tgaggagccg
     2221 cggatgatcc cccgccgcgc tccggcctcg ctgtatccgg ccatcctgat actcgtgggg
     2281 ctggccgcct ccgcgaccgg ggcctgggca caggcgggag gcacctcctc cccgtcggcc
     2341 ccc
//
LOCUS       DASVOD010000787         7603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_729758, whole genome shotgun sequence.
ACCESSION   DASVOD010000787 DASVOD010000000
VERSION     DASVOD010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729758"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VF720_16830"
     CDS             <1..915
                     /locus_tag="VF720_16830"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX7881078.1"
                     /translation="DKSVYMLGEDIGQYGGAFKVTKGFLDEFGPERIVDTPLAESAII
                     GVAIGSAMMGMRPVAEMQFADFVACGFNQLINNAAKMHYRWGAAVPMVVRLPSGGGVR
                     GGPFHSQNPEAWFCHTPGLKVVAPSTPNDAKGLIKAAIRDDNPVLYFEHKYLYRRIKD
                     EVPDGDFTIEIGKADIKRPGADVSIITYGSMVHFSLEAAEKLAGEGIDVEVVDLRSLL
                     PYDQQAIADTVRKNNRVVVVHEATRTCGFAAEVMAFIVEDLFTHLDAPVKRVTSIDAP
                     VPYSPPLEDFFMPNADKIAAAVREVAAF"
     gene            956..2428
                     /locus_tag="VF720_16835"
     CDS             956..2428
                     /locus_tag="VF720_16835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014201366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX7881079.1"
                     /translation="MARVDIIMPQLGESIAEGTVVKWLKKPGDKVGKDESVLEITTDK
                     VDSEIPSPSAGILVEIVAPEGTTVGIGKPLGVLETDATAAAGASKAAPAPEAAKPTSD
                     VTKAPVTAGDSVVPGAGAAAPMAAPPPEPAVMAHAGHGNGGNGGSDRFYSPLVKRMAR
                     EEGVALSELDRLAGTGQGGRVTKDDFLGYLEQRKAGGVAMPQAQPMGQPTMMPAGMPM
                     MPMMPQFAPMPQFMPMPGQSGPPAVPAAYKINREPGAPETRIPWDNVRKKIAAHMVMS
                     KSTSPHVTSCAEVDVTTIVSFRETYKDVFQQSQGFKLTYTPFFLHAVTKAIQDFPGVN
                     AIVDGETTVIKHAINLGMAVAMDDGGLIVPVLKHAEDKNFVGLARNADDLGRRAKSKK
                     LVPDDISGGTFTITNPGVFGGLWGTPIINQPQVAIFGMGTINKRVAVINNMIAIRDMI
                     YVTLSYDHRVVDGALSHLFLARVKWYLENFNPMGQIPGVI"
     gene            2444..3463
                     /gene="lipA"
                     /locus_tag="VF720_16840"
     CDS             2444..3463
                     /gene="lipA"
                     /locus_tag="VF720_16840"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007803982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX7881080.1"
                     /translation="MSDIDRPDDTQVERGKAPLRRSLIRPDSADQFKWNDRGRSAAES
                     STPATPNVNIPGHRPAWLKAKLPGGATFNEMKALVASHKLNTVCQSANCPNIGECWNE
                     RHVTLMIMGHTCTRSCGFCDVLTGRPAALDAGEPARVADAIRIMGLDHVVITSVNRDE
                     LPDGGAAHWAATIREVRRVNDAITVEVLVPDFQGDTAAVNTVLAARPHVYAHNLETVR
                     RLQKVVRPQASYGTSLMTLRHAAGPGHLTKSGLMAGLGETEDELREALRDLREAGVAV
                     VTVGQYLQPSPKHLPVARWVTPEEFARLKEFGEALGIRHVESGPLVRSSYRAGRQVRE
                     LLTAR"
     gene            complement(3499..4137)
                     /locus_tag="VF720_16845"
     CDS             complement(3499..4137)
                     /locus_tag="VF720_16845"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX7881081.1"
                     /translation="MNPRTSVLVVALAAMVSVLPRSASALPPIDRHNWSAGVAYGVGH
                     ADIDFVNQDITVDGERGGTPQFHISRMIGSRFMLGIANRQWMDEGGLYEGKIRCNTQS
                     LSLMLTVYPGNLANWTSGFYLQGGYGFANARISLLERAEGGVDEHGNTYVVVRKEDEG
                     GTAVGLGAGYEFRISSHFAAGVNVSYNQLSINGEIFDGANFVPGGIHLNWYF"
     gene            4227..5600
                     /locus_tag="VF720_16850"
     CDS             4227..5600
                     /locus_tag="VF720_16850"
                     /inference="COORDINATES: protein motif:HMM:NF024484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3943 domain-containing protein"
                     /protein_id="HEX7881082.1"
                     /translation="MFRRRALVALLLLTLPAMARAQESAPPDTTVVEFDHPKPLRALG
                     EVVGTNLAIWTYDRYIRENGTNPGFRIGFNSWEENMLNGFEWDDNNFKTNQFAHPYHG
                     SMYFNAARSSGYDFWTSIPFAFTGSILWEYMFEIHHPSYNDWIATSVGGTTLGEMMHR
                     LSSLILDDRDTGGSRRWRELGALLVDPMRGLNRIIDGDVSRVGPNPSDWRPGYLGMRF
                     DAGLRVTGEDKLTDADTTRAFISLEGQYGDPFGRDRRKPFDTFGFGVDLNFGDASVLS
                     GVRARGLLAAADLKRSEKTNHLLGAFLNFDYANTNAYTLGGQSIGAGVMSRFDDTFLG
                     DIRTEIHANAVILGAVKSDYESYTGRAYDYGPGASAIIAASLVRNNRPWLSVRHSEVW
                     IHTLNGTDGEHFVSVTRARVDVPIAPTLGVGAMYQLYLSDADYTDLEDTHHRLPELRA
                     FLSFPLD"
     gene            5614..6006
                     /locus_tag="VF720_16855"
     CDS             5614..6006
                     /locus_tag="VF720_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881083.1"
                     /translation="MKLKLTALVAFGVLAMAPAAMAQGNSSDWTDLGSPRMKGRLGVA
                     EVSFDTLMVHEVQFAYDDASNFRIGQVTIMHGPRHKPWPQDFLAIDPLPGSPRIMRLR
                     KPVRATEIRFLYSNLIPGEKSDVHVLVR"
     gene            complement(6003..7118)
                     /locus_tag="VF720_16860"
     CDS             complement(6003..7118)
                     /locus_tag="VF720_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881084.1"
                     /translation="MTALAALLLPAFAAPAPAAPRIVSQTKVSGVPGTIVAWYDSMAY
                     VLGRDSTNAPALIQYWPESGRQISLVLGSRRLGTFLSVAPDGHTILVRETDSTGVNRL
                     LTTTLPTKPPATGSYSALWIDHLPVSDADPHVTWYGDSKSFLYQDVLDDAKSTPSVFR
                     FRLGDKLPRLYLFGCSRPLLAPSGDAVVCVGVDTLNPGQPRDLESRQPVGMEDIKAGD
                     FHWVAPLRTWIPSRAGWSPDGNRIALVGYEIAADATLLRKLYIHSRITRGNTQLDLKG
                     DNHRPDRDHSQDVACWSPDGSWIAVVMTSPRSGEPEYKGGVWITTKSGQEPTQLVPTE
                     GVWRGNLFWVGPKTFLIGDIEPPPGMERTYWRVEVAG"
     gene            complement(7163..>7603)
                     /locus_tag="VF720_16865"
     CDS             complement(7163..>7603)
                     /locus_tag="VF720_16865"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX7881085.1"
                     /translation="VDFKLEMAKTMGATEVFRVDQPGVEEAIRKATGGEGVDCVLEMS
                     GAPAGVNFGLKLLRMGGWIGLLGLPKKPFEIDVTRDIIFKGVTVHGIIGRRMYDTWYQ
                     GTALIADRGLDLSPVITHELPLDRFEEGFAAMTAGRAGKVILYP"
BASE COUNT         1370 a         2550 c         2500 g         1183 t
ORIGIN      
        1 gacaagagcg tgtacatgct cggcgaggac atcgggcagt acggcggcgc cttcaaggtg
       61 acgaagggat tcctcgacga gttcggcccc gaacggatcg tggatacgcc cctggccgag
      121 agcgccatca tcggcgtggc catcggctcg gccatgatgg ggatgcgtcc cgtggccgaa
      181 atgcagttcg ccgacttcgt ggcgtgcggc ttcaaccagc tcatcaacaa cgcggccaag
      241 atgcactacc gctggggcgc ggccgtcccg atggtggtgc ggctgccgtc gggcggcggc
      301 gtgcgcggcg gccccttcca cagccagaac cccgaagcct ggttctgcca cacgccggga
      361 ctcaaggtgg tggctccctc cacgccgaac gacgccaagg ggctcatcaa ggccgccatc
      421 cgcgatgaca acccggtcct ctacttcgag cacaagtatc tctaccggcg catcaaggac
      481 gaggttccgg acggggattt cacgatcgag atcggcaagg ccgacatcaa gcggccgggg
      541 gcggatgtct cgatcatcac ctacgggtcg atggtccact tcagcctgga agccgccgag
      601 aagctggccg gcgagggaat cgacgtcgag gtcgtggacc tgcgctcgct cctcccctac
      661 gaccagcagg ccatcgccga cacggtgcgg aagaacaacc gggtggtggt ggttcacgag
      721 gccacccgca cctgcggctt tgccgccgag gtcatggcct tcatcgtcga ggatctcttc
      781 acgcatctcg acgctcccgt gaaacgagtc acgtccatcg acgcgccggt gccgtacagc
      841 ccgccgctcg aggatttctt catgcccaac gccgacaaga tcgccgcggc ggttcgcgaa
      901 gtcgcggcgt tttagatttt ggcaaaccgg aagcagcgat aacaaaggag cagacatggc
      961 acgcgtagac atcatcatgc cgcagctcgg tgagtcgatc gccgagggga cggtggtgaa
     1021 atggctcaag aagccgggcg acaaggtggg gaaggacgaa tcggtcctcg agatcaccac
     1081 cgacaaggtc gactcggaga tcccatctcc ttcggccggc atcctcgtgg agatcgtggc
     1141 ccccgaaggg acaacggtcg gcatcggcaa gccgctcggc gtcctcgaga ccgatgccac
     1201 cgcggcggcc ggcgcgtcca aggcggcacc cgcgccggaa gccgccaagc cgacgagcga
     1261 cgtgacaaag gccccggtca cggccggaga cagcgtggtg cccggcgccg gcgccgctgc
     1321 gccgatggcc gcccccccgc cagaaccggc cgtcatggcc catgccggcc acggcaatgg
     1381 cggcaacggc ggttccgatc gcttctactc gccgctggtc aagcggatgg cgcgcgaaga
     1441 gggcgtggcc ctttccgaac tcgatcgcct ggccggtacc ggccaggggg gccgcgtcac
     1501 caaggatgat ttcctcggct acctcgagca gcgcaaggcg ggtggcgtgg ccatgccgca
     1561 ggcgcagccg atgggccagc ccacgatgat gccggccggc atgccgatga tgcccatgat
     1621 gccgcagttc gcgccgatgc cgcagttcat gccgatgccg gggcagtcgg gcccgccggc
     1681 ggttccggcg gcctacaaga tcaaccgcga gcccggagcg ccggaaaccc gcatcccctg
     1741 ggacaacgtg cggaagaaga tcgccgcgca catggtgatg tcgaagtcga ccagcccgca
     1801 cgtcacctcg tgcgccgaag tcgacgtgac caccatcgtg tcgttccgcg agacctacaa
     1861 ggacgtgttc cagcagtcgc agggcttcaa gctgacttac acgcccttct tcctgcacgc
     1921 ggtgacgaag gccatccagg acttcccggg ggtcaacgcc atcgtcgatg gggaaacgac
     1981 ggtcatcaag cacgccatca acctcggcat ggccgtggcc atggatgacg gcggcctgat
     2041 cgtgcccgtt ctcaagcacg cggaagacaa gaacttcgtg ggcctggccc gcaacgccga
     2101 cgatctcggc cgtcgcgcca agtcgaagaa gctggtgccg gacgacatct cgggcggcac
     2161 gttcaccatc accaacccgg gcgtcttcgg cggcctgtgg ggcacgccga tcatcaacca
     2221 gccgcaggtg gccatcttcg ggatgggcac gatcaacaag cgggtggcgg tgatcaacaa
     2281 catgatcgcc atccgggaca tgatctacgt gacgctgtcg tacgaccatc gcgtggtgga
     2341 cggggcgctc agccacctct tcctggcgcg cgtgaagtgg tacctcgaga acttcaaccc
     2401 catgggacag atccccgggg tcatctgagc aaggaaaggc agtatgtccg acatcgatcg
     2461 tccggacgac acgcaggttg aacgtggtaa ggctccgctc cggcggagcc tcatccgtcc
     2521 ggacagcgcc gaccagttca agtggaacga ccgtggccgc tcggccgcgg agagcagcac
     2581 ccccgccacg ccgaacgtga acatcccggg gcaccgcccc gcctggctca aggcaaagct
     2641 acccggcggc gccaccttca acgagatgaa ggcgctcgtg gccagccaca agctcaacac
     2701 cgtctgccag agcgccaact gtcccaacat cggcgagtgc tggaacgaac ggcatgtcac
     2761 gctgatgatc atgggtcaca cctgcacccg ctcgtgcggc ttctgcgacg tgctcaccgg
     2821 ccgtcccgcc gcgctcgacg ccggcgagcc cgcccgggtg gccgatgcca tccggatcat
     2881 ggggctcgat cacgtcgtca tcacgagtgt gaaccgggat gaactccccg acggcggcgc
     2941 ggcgcactgg gccgcaacca tccgggaagt acggcgcgtc aatgacgcca tcaccgtcga
     3001 ggtgctggtg cccgacttcc agggggatac tgcggcggtc aacacggtcc tggccgcccg
     3061 cccgcacgtg tacgcgcaca acctggaaac ggtgaggcgc ctgcagaaag tggtgcgccc
     3121 ccaggccagt tacggcacga gcctcatgac gctgcgccat gccgccggcc ccggccatct
     3181 caccaagtcc ggcctgatgg ccggactcgg tgaaacggaa gacgaattgc gcgaagccct
     3241 ccgcgacctg cgtgaggctg gcgtggccgt cgtcacggtg ggacagtacc tgcagccatc
     3301 accgaagcac ctgccggtcg cgcggtgggt aacgcccgag gagtttgcgc gactcaagga
     3361 gttcggcgaa gcgctcggca tccggcatgt ggaatcgggg ccgctggtgc ggtcgagcta
     3421 tcgggcgggg cgccaggtgc gggagctgct cacggcgcgg tagggatctg ccgcagggga
     3481 aaagaacccc gcgcggccct agaaatacca gttcagatga atgccgccgg gaacgaaatt
     3541 ggccccgtcg aagatctcgc cattgatcga gagctggttg tagctgacat tgacgccggc
     3601 cgcaaagtgg ctggaaatgc ggaactcgta gccggcgccg agccccacgg ccgtgccgcc
     3661 ctcgtcttcc ttgcggacca ccacgtaggt gttgccatgt tcgtccacgc caccctcggc
     3721 ccgctcgagg agcgagatgc gggcgttggc gaaaccgtag ccaccctgga ggtagaagcc
     3781 gctcgtccag ttcgccaggt ttccggggta gacggtgagc atgagcgaca ggctctgggt
     3841 gttgcaccgg atcttgccct cgtagaggcc cccctcgtcc atccactggc gattggcgat
     3901 gccgagcatg aagcggctgc cgatcatgcg gctgatgtgg aactgcgggg tgccgccgcg
     3961 ctcgccgtcg accgtgatgt cctggttgac gaaatcgatg tcggcgtgcc ccacaccgta
     4021 ggcaacgccg gcagaccagt tgtggcggtc gatcgggggg agggcgctgg ccgagcgggg
     4081 caacactgac accatcgccg ccagtgcgac gacaaggacg gaggtccggg ggttcatggc
     4141 cgaaccatag caccggcacg gggggttgaa aaccggtaat cggccggagg cttgtcatcc
     4201 caaatccttc gtcttaggat gcccgcatgt tccgccgacg cgcgctcgtc gcgctcctcc
     4261 tcctgacgct ccctgccatg gcacgggcgc aggaatccgc cccccccgac acgacggtcg
     4321 tcgagttcga tcatcccaag cccctgcgcg ccctcggcga agtggtcggc accaacctgg
     4381 ccatctggac ctacgaccgt tacatccgcg agaacggcac gaaccccggc ttccggatcg
     4441 gcttcaactc gtgggaagag aacatgctga acgggttcga gtgggacgac aacaatttca
     4501 agacgaacca gttcgcccac ccctatcacg gcagcatgta tttcaacgcg gcgcgctcga
     4561 gcggctacga cttctggacg tcgatcccgt tcgcgttcac gggcagcatc ctctgggagt
     4621 acatgttcga gatccatcac ccctcgtaca acgactggat cgccaccagc gtggggggca
     4681 cgacgctcgg cgagatgatg caccggcttt cgagcctgat cctcgacgac cgtgacacgg
     4741 ggggcagccg ccggtggcgc gagctcggcg ccctcctcgt cgacccgatg cgcggcctga
     4801 accgcatcat cgacggcgac gtgagccggg tgggaccgaa cccctccgac tggcgccccg
     4861 gctacctcgg gatgcgtttc gacgccgggc tccgggtgac cggcgaggac aagctcacgg
     4921 acgccgacac gacccgggcc ttcatctcgc tcgagggaca gtatggcgat cctttcggcc
     4981 gcgaccggag gaagcccttc gacacgttcg gcttcggggt cgacctcaac ttcggcgacg
     5041 ccagcgtcct ttccggcgtg cgcgcccggg gcctcctggc cgccgcggac ctgaagcgca
     5101 gcgagaagac gaaccatctc ctgggcgcct tcctcaactt cgactacgcc aacaccaatg
     5161 cctacacgct tggcggccag agcatcggcg ccggcgtcat gtcccgcttc gacgacacgt
     5221 tcctcggcga tatccggacc gagatccatg ccaacgcggt catcctcggg gcggtcaagt
     5281 ccgactacga gagttacacc gggcgcgcat acgattacgg cccgggggcc tcggccatca
     5341 ttgcggcctc actggtgcgg aacaaccggc cctggctatc ggtgcggcac tcggaggtgt
     5401 ggatccacac gctgaacggc accgacggcg agcacttcgt gtcggtgacg agggcccggg
     5461 tggacgtgcc gatcgcaccg acgctcgggg tgggtgccat gtaccagctc tacctgtcgg
     5521 atgccgatta caccgacctg gaagacacgc accaccgcct gcccgaactg cgcgccttcc
     5581 tctccttccc gctcgactga taaggagacg aacatgaagt tgaagctgac ggcgctcgtg
     5641 gccttcggtg tgctcgcaat ggctccggcc gccatggccc agggaaactc ctcagactgg
     5701 accgatctcg ggagcccgcg gatgaagggg cgcctgggcg tggccgaggt gtcgttcgac
     5761 accctcatgg tccacgaggt gcagttcgcc tacgacgacg cctcgaactt ccggatcgga
     5821 caggtgacga tcatgcacgg tccgcgccac aagccgtggc cgcaggactt cctggcgatc
     5881 gacccgctgc cgggctcccc gcgcatcatg cggctcagga agcccgtccg cgccaccgag
     5941 atccggttcc tctactcgaa cctgattccg ggcgaaaagt cggacgtcca cgtcctcgtt
     6001 cgctaaccgg ccacttccac ccgccagtag gtccgttcca tccccggcgg gggctcgatg
     6061 tcgccgatca ggaaagtctt cggacccacc cagaaaaggt tgccgcgcca gacgccctcg
     6121 gtcggcacca gctgcgtcgg ctcctggccc gacttggtgg tgatccacac gccacccttg
     6181 tactcgggtt cgccggaccg ggggctcgtc atcacgacgg caatccaact gccgtcgggc
     6241 gaccagcagg ccacatcctg tgagtggtcg cgatcggggc gatggttatc ccccttcagg
     6301 tcgagctgcg tgtttccgcg cgtgatgcgg gagtggatgt agagcttgcg cagcagcgtg
     6361 gcatcggccg cgatctcgta accgaccagc gcgatgcggt tgccatcggg cgaccacccg
     6421 gcccgcgagg gaatccaggt gcggagcggc gccacccagt ggaaatcgcc ggccttgatg
     6481 tcttccatgc cgaccggctg gcgcgattcc aggtcacgcg gctgcccggg gttcagcgtg
     6541 tcgaccccga cgcagacgac ggcgtcgccc gatggcgcca ggagaggccg actgcacccg
     6601 aacaggtaga gcctggggag cttgtccccc agccggaagc ggaagaccga cggggtggat
     6661 ttcgcgtcgt ccagcacgtc ctggtagagg aacgacttcg agtcgccgta ccaggtgacg
     6721 tgcggatcgg cgtcgctgac cggcaggtga tcgatccaga gagcggagta ggagccggtg
     6781 gcggggggct ttgtgggaag cgtggtggtg agaaggcgat tgaccccggt ggaatcggtc
     6841 tcccgcacca ggatggtgtg gccgtccggc gccaccgaaa ggaacgtgcc gagacggcgc
     6901 gatccgagca cgagcgagat ctgtctcccg ctctcgggcc agtactggat gagcgccggg
     6961 gcgttggtcg aatcgcgccc gaggacgtag gccatcgagt cgtaccaggc cacgatggtg
     7021 ccgggtacac cgctcacctt cgtctggctc acgatgcggg gagcggcagg ggccggggcc
     7081 gcgaaggccg gcaggagaag tgcggcgagg gcggtcacga gtgcgccgcg acgggatcga
     7141 ttccggatca cgagtgacgc gctcaggggt agagaatgac cttcccggca cggccggccg
     7201 tcatggccgc aaagccttcc tcgaaccgat ccagcggaag ttcgtgcgtg atgaccggcg
     7261 agaggtcgag gccgcgatcg gcaatgagcg ccgttccctg gtaccaggtg tcgtacatcc
     7321 gccggccgat gatcccgtgc accgtgacac ccttgaagat gatgtcgcgc gtcacgtcga
     7381 tctcgaacgg cttcttcggc aggccgagca ggccgatcca gccccccatg cgcaggagct
     7441 tgaggccgaa gttcacgccg gccggagcgc cggacatctc gagcacgcaa tcgacaccct
     7501 cgcccccggt ggccttccgg atggcctctt cgacgcccgg ctgatcgacg cggaacacct
     7561 cggtggcgcc catcgtcttc gccatctcca gcttgaaatc cac
//
LOCUS       DASVOD010000788         3856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_626087, whole genome shotgun sequence.
ACCESSION   DASVOD010000788 DASVOD010000000
VERSION     DASVOD010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626087"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VF720_16870"
     CDS             complement(<1..581)
                     /locus_tag="VF720_16870"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX7881086.1"
                     /translation="MREAVALFPGQGSQSVGMGRALFEECPEARSVFEAADDALGFAL
                     SRLAFEGPDEELTLTRNTQPAILMHSVAALRVLAARGFAPTAAAGHSLGEYSAYVAAG
                     SLTFEDAIRLVRRRGELMYEAGVARPGAMAAVLGLTDSQVEEVCRDAADTGIVVPANR
                     NSPGQVVLSGEEAAVERAMELAMMAGAKKSVRLP"
     gene            complement(581..1576)
                     /locus_tag="VF720_16875"
     CDS             complement(581..1576)
                     /locus_tag="VF720_16875"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX7881087.1"
                     /translation="MADRKTTCVIAGTGSFAPPRILSNHDLERLVETSDEWITTRTGI
                     RERRIADPGVASSDLAFEAAERALRTAELEARDIDVIIVGTVTPDHVFPSCGCTLQAR
                     LGARNAAAFDVTAACSGFLYALQIGRSMIEAGTARNVLIVGVEVLSRIIDWSDRSTCV
                     LFGDGAGAVVLRPSDEPGRGVLSVRIGSDGEQGEVLYLPAGGSRHPATHETVEQHLHY
                     VHMSGNDLFKIAVRGMETVARQALDEAGIGISDIDLLVPHQANLRIIDATARRLEVPS
                     EKVVVNIDRYGNTSAASIPIALDEAVRGGRIRPGSRVVMVAFGGGLTWGAAVVNW"
     gene            complement(1569..2615)
                     /gene="plsX"
                     /locus_tag="VF720_16880"
     CDS             complement(1569..2615)
                     /gene="plsX"
                     /locus_tag="VF720_16880"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX7881088.1"
                     /translation="MNAKRPITVGVDGMGGDEGPGVIVEGALMALREMDAEGRVALIG
                     DQRVLEAEVRRFDPSGSAGSRLLIEHAAEAVGMGEEAATAARRKKESSLAVGTRLMKE
                     GRIDALFSAGNTGAMVAASLLGVGRIPGVQRPALATRVPADTPSGFCVILDVGATADC
                     KPAHLAQFAVMGDIYTRRILGVAEPRVGLLNIGEESSKGNELAREAHALMTGLPIRFA
                     GNVEGRDIFRGHADVVVTDGFTGNIVLKFLESVAGWTSRILKDEIKSNLVSQLGALLL
                     MPGLKGLRRRLDYSEYGGAPLLGVNGACIVGHGRSSAKAVKNGLKAGAAFVRESVNDR
                     IHEAFGKERAEHVG"
     gene            complement(2612..2794)
                     /gene="rpmF"
                     /locus_tag="VF720_16885"
     CDS             complement(2612..2794)
                     /gene="rpmF"
                     /locus_tag="VF720_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020267997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEX7881089.1"
                     /translation="MALPKRRHSHTRGAKRRTHWKLKATSLSTCSHCTQPKLPHRVCP
                     NCGYYDGVEVITPKES"
     gene            complement(2878..3438)
                     /locus_tag="VF720_16890"
     CDS             complement(2878..3438)
                     /locus_tag="VF720_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEX7881090.1"
                     /translation="MSRHDHRNDRKSLKRLILPISPLETGRSPLEMSVDLNRLELGDW
                     VRPLEPMELEGSVDKFGEAVTVRGHGEARVEETCARCTGTFERILAIDILVYCDRRGS
                     DAEADTRQLEQEGEVVYHDGVSVDLTEPVREAVILAQPMNPLCREDCKGLCAGCGADL
                     NVEPCRCGEPAPDPRWAALGRLKSGE"
     gene            complement(3477..>3856)
                     /gene="ndk"
                     /locus_tag="VF720_16895"
     CDS             complement(3477..>3856)
                     /gene="ndk"
                     /locus_tag="VF720_16895"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016960892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX7881091.1"
                     /translation="DAAERNLVGSILSKIEASGLKIVRLQMLQLTPERARKFYHVHEG
                     KPFLDDLVAYMSRGPVVVGALAGESAITRWRELMGATNPANAAPGTIRKEFAVDIQTN
                     TVHGSDSPASAAEEIPFFFPNPL"
BASE COUNT          586 a         1284 c         1349 g          637 t
ORIGIN      
        1 gggaggcgga ccgacttctt cgcgccggcc atcatggcca gttccatggc cctctccacc
       61 gccgcctctt cacctgacag gaccacctgc cccggcgagt tccggttggc cgggacgacg
      121 atgccggtgt cggcggcatc gcggcagacc tcttcgacct ggctgtcggt gagcccgagg
      181 acggcggcca tcgcaccagg acgggcaacg cccgcctcgt acatcaactc cccccgccgc
      241 cggacgagcc ggatggcgtc ttcgaacgtc agcgacccgg ccgccacgta cgccgagtat
      301 tcgcccagcg agtggccggc ggccgcggtg ggcgcgaagc cacgggcggc gaggacacgc
      361 agcgccgcca cgctgtgcat gaggatggcc ggctgggtgt tccgggtgag cgtgagctcc
      421 tcgtcggggc cctcgaacgc gaggcgcgac agtgcgaagc cgagcgcgtc gtcggcggcc
      481 tcgaagacgg agcgggcctc gggacactcc tcgaagagcg cccggcccat cccgaccgac
      541 tgtgatccct gccccggaaa gagggcgacc gcttcgcgca tcaccagttc accaccgcgg
      601 cgccccaggt gagtccgccg ccgaacgcca ccatcacgac ccgggaaccg ggtcggatgc
      661 gacctccccg gaccgcctcg tcgagggcga tggggatcga ggcggcggac gtgttgccgt
      721 accggtcgat gttgaccacc actttctccg aaggaacttc gaggcgtcgt gccgtggcgt
      781 cgatgatgcg gagattggcc tggtggggca cgaggagatc gatgtccgag atgccgatcc
      841 cggcttcatc gagggcctgg cgggccacgg tctccatgcc ccggacggcg atcttgaaca
      901 ggtcgtttcc gctcatgtgc acgtagtgca ggtgctgctc gaccgtttcg tgagtggctg
      961 gatggcggga gccgccggcc gggaggtaaa gcacctctcc ctgttcgccg tcggaaccga
     1021 tccggaccga gaggacgccg cggccgggct cgtcagacgg cctcaagacg acggcgccgg
     1081 cgccgtcgcc gaacagcaca caggttgagc ggtcggacca gtcgatgatg cgcgagagga
     1141 cctcgacgcc gacgatgagg acgttccggg ccgttccggc ctcgatcatc gagcgtccga
     1201 tctgcagggc gtagaggaaa cccgagcagg cggcggtgac gtcgaacgcg gcggcgttcc
     1261 gggccccgag ccgcgcctgc agcgtgcagc cgcacgacgg aaagacgtga tccggcgtga
     1321 ctgtgccgac gatgatgacg tcgatgtccc tggcctcgag ttcggccgtg cggagcgcac
     1381 gctcggcggc ctcgaaggcg agatcggacg atgccacgcc cggatcggcg atgcggcgtt
     1441 cgcggatccc cgtgcgggtg gtgatccatt cgtcactggt ttcgacgagg cgctccaggt
     1501 cgtggttcga caggatccgc ggcggggcga acgatccggt gccggcgatg acgcaggtgg
     1561 tcttgcgatc agccaacgtg ttccgccctt tccttcccga aagcctcgtg gatgcgatcg
     1621 ttgacgctct cccggacgaa ggccgccccg gccttgaggc cgttcttgac cgccttggca
     1681 ctggaccgcc cgtggcccac gatacaggcc ccgttgacgc cgagaagcgg cgcgccgccg
     1741 tattcggaat agtcgagccg gcgtcgaagc cccttgaggc ccggcatgag gaggagcgcc
     1801 ccaagttggg aaacgaggtt ggactttatc tcgtccttca ggatccggct ggtccagccg
     1861 gccacgctct cgaggaattt gaggacgatg ttgccggtga agccgtcggt gacgaccacg
     1921 tcggcatggc cccgaaagat atcgcgaccc tcgacgttcc cggcgaagcg gatcggcagg
     1981 ccggtcatga gggcgtgagc ctcgcgggca agctcgttcc ccttcgatga ctcttcgccg
     2041 atgttgagca ggccgacccg cggctcggcc acgcccagga tgcgccgtgt gtagatgtcg
     2101 cccatgacgg cgaactgggc caggtgggcc ggcttgcagt cggccgtggc gccaacgtcg
     2161 agtatcacgc agaagccgct cggcgtatcg gcggggacgc gcgtggccag cgccggccgc
     2221 tggacgccgg gaatgcggcc gacgccgagc agcgaggcgg ccaccatggc gccggtgttg
     2281 ccggccgaaa agagggcgtc gatccgccct tctttcatga ggcgcgtacc gaccgccaga
     2341 gacgattcct tcttgcggcg cgccgccgtg gcggcttcct cacccatgcc gacggcctcg
     2401 gcagcgtgct cgatcagcag tcgtgaaccg gccgagccgg atggatcgaa acggcgtact
     2461 tcggcctcga ggacgcgctg gtcgcctatc aaggccaccc ggccctcggc gtccatctcc
     2521 cggagggcca taagggcccc ctcgacgatg acgccggggc cctcgtcccc gcccatgcca
     2581 tccactccga ccgtgatcgg ccgcttcgcg ttcacgattc cttgggggtg atgacctcga
     2641 cgccgtcgta gtagccgcag ttggggcaca cccggtgcgg cagcttgggc tgtgtgcagt
     2701 gcgagcaggt ggacaggctg gtggccttga gcttccagtg ggtccggcgc ttggctcccc
     2761 gcgtgtggga gtgccggcgt ttcggcagtg ccatggttcg ttctctcctt gcgtcgtgga
     2821 ccttgcggcc cggtggtcgt tgttcgtttc aaaaggcgcc ggcgcgccga cttgccgcta
     2881 ctcgccggac ttgaggcgac cgagcgcggc ccagcgcggg tcgggcgccg gttctccgca
     2941 ccggcagggc tcgacgttga ggtcggcgcc gcaaccggcg cacaacccct tgcagtcttc
     3001 acggcagagc gggttcatcg gttgggcgag gatgacggct tcccggacgg gttcggtcag
     3061 atcgacactg accccgtcat ggtagacgac ctccccctcc tgttccagtt ggcgggtgtc
     3121 ggcctcggca tcgcttcccc gacggtcgca atagaccagg atatcgatgg ccaggatgcg
     3181 ttcgaacgtg ccggtgcagc gggcgcaggt ttcctctaca cgcgcctccc cgtgaccccg
     3241 caccgtaacc gcctcgccga acttgtcgac cgaaccttcg agctccatcg gctcgagcgg
     3301 ccggacccaa tcgccgagtt cgaggcgatt caggtcgacg gacatctcga gcggcgaccg
     3361 ccccgtctcc aggggagata tggggaggat caaccgcttg agcgatttcc tgtcgtttcg
     3421 gtggtcgtgc cgggacatgc tggctcgttc gtggttttcc gtccgggccg cgaggctcag
     3481 agcgggttcg ggaaaaagaa agggatctcc tcggcggcgg acgccggcga gtcggaaccg
     3541 tggacggtat tggtctggat gtcgaccgcg aactccttgc ggatggtgcc gggcgcggcg
     3601 ttggccgggt tggtggcgcc catcagttca cgccagcgcg tgatggcgct ctcccccgcc
     3661 agggcgccga ccacgaccgg tccgcgcgac atgtaagcca caaggtcgtc gaggaacggc
     3721 ttcccctcgt gcacgtggta gaacttgcgc gcgcgctcgg gcgtgagctg gagcatctgg
     3781 agccgcacga tcttgaggcc ggaagcctcg atcttcgaaa gaatgctgcc gaccaggttt
     3841 cgctcggcgg catcgg
//
LOCUS       DASVOD010000789         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_365222, whole genome shotgun sequence.
ACCESSION   DASVOD010000789 DASVOD010000000
VERSION     DASVOD010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365222"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VF720_16900"
     CDS             complement(<1..1199)
                     /locus_tag="VF720_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881092.1"
                     /translation="MRLVRFARGDFLERTRNSGFVVTLVAILAAASLGLPTNSSSYAT
                     VDLDGWRGLYDSAWVGVSVSLLTSLFLSLIGFYLVKNSLERDRRSGVGQLLGASPLSR
                     FGYLMGKMLSNAALLGVLVFAVMVMAGVMQYLRGESRLLQPVDLVAPFLLVTLPFLFL
                     VAAFAVLFESVPLLRGSLGNVAWFFLWTALLVGPGMENAELARTGGEPGNDPMGASFL
                     IGEMMRGARAAVPGLDPKNISVGVNITPGRTLQTFDWGGIDWTAARVFGRLMWLLGVP
                     VIVGVAVPFFDRFSGEKRRADAPGEPVGGAATPIPSLPMEGGAFSAAALTAIGARASS
                     PDVARLVSAEVKLLVKGVRRIWWLVALGLSIASAVAPGTGGAVLAAIVWIWPLAIWSG
                     MGCRERL"
     gene            complement(1196..2074)
                     /locus_tag="VF720_16905"
     CDS             complement(1196..2074)
                     /locus_tag="VF720_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016427926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX7881093.1"
                     /translation="MKLDFEGLGKRYGRDHWALREFSLSLAPGIVGLLGPNGAGKSTL
                     MKILATVSRATEGRVLWNGEDIATKPDPLRRVLGYLPQDFGIYPNLTALEFLDYLAAA
                     RGLSSAVSKKRIEELLALVNLSEAAKRAIGGYSGGMRQRLGIAQALLNDPKLLIVDEP
                     TVGLDPEERNRFRHLLTELAGSRIVLLSTHIVSDVEATAESIALIHRGRLVAFSSPEE
                     LMARASGQVWEWSVAAGELPDVRRRHRVSGALRRGGDVLVRVVGERPAGGALPATPTL
                     EDAYLCHVEPASAGSA"
     gene            2263..3063
                     /locus_tag="VF720_16910"
     CDS             2263..3063
                     /locus_tag="VF720_16910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020034819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEX7881094.1"
                     /translation="MPQMHEVDYKVYGEDLQFVEVELDPEEAVVAEAGGMMYMDDGIM
                     METIFGDGSAQSQGKGFLGSLMGAGKRLLTGESLFMTVFYNKGAGKKKVAFGAPYPGT
                     ILPMHLGQMGGELIVQKDSFLCAAKGVSVGISFQKKLGAGLFGGEGFIMQRLTGDGLA
                     FIHAGGTLYARTLNPGEVLRVDTGCIVGFQPSVDYDIQMVGGVKSALFGGEGLFFATL
                     RGPGQIWLQSLPLNRMAARIIAAGGSVGGRREEGSILGGLGNLLDGDS"
     gene            complement(3311..4030)
                     /locus_tag="VF720_16915"
     CDS             complement(3311..4030)
                     /locus_tag="VF720_16915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010036021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX7881095.1"
                     /translation="MNEGPTMKLKTLRDLYVEELKDIYSAENQILATLPRLIDGAELP
                     ELKAAFEDHLGQTREHVNRLETIFESLDVSPRGKKCRGMEGLLEEGSELLQENEDGST
                     VLDAGLIGAAQRVEHYEMAAYGCARAHAHELGLDDAADLLQQTLDEEEAADMRLTEIA
                     EQAANARAAISDRAQSKQREDDLDGLDDEEEFDVPTVARRAAPRATSSPASKSKGATK
                     SAVTKRANGNRTSVPMATKRK"
     gene            4271..>4601
                     /locus_tag="VF720_16920"
     CDS             4271..>4601
                     /locus_tag="VF720_16920"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX7881096.1"
                     /translation="MAESIKTYLMFDGTAEAAIRLYVSLFAGAEITRLELRGESEPGK
                     PGTVSRGTFRLLGQEYMAFDTPMPHAFGFTPAISLFVECDSEEELDRLHAALSEGGTE
                     MMPPGDYG"
BASE COUNT          813 a         1575 c         1489 g          724 t
ORIGIN      
        1 tgaagtcttt cgcggcaccc catgccggac cagatggcca gcggccagat ccacacgatc
       61 gccgccagca ccgccccgcc ggtgcccggg gccaccgcgg aggcgatcga caaccccagc
      121 gccaccagcc accagatccg gcggacaccc ttcacgagaa gtttcacttc ggcgctgacg
      181 agccgcgcga catcgggcga cgatgcgcgc gccccgatgg cggtgagggc cgccgccgaa
      241 aaggcgccac cctccatcgg cagcgacgga atcggcgtcg ccgcgccacc caccggttcg
      301 ccaggcgcat ccgcccggcg cttctctccc gagaatcgat cgaagaacgg caccgccaca
      361 cccacgatga ccggcacccc aagaagccac atcaagcgcc cgaacacgcg ggccgccgtc
      421 cagtcgatgc cgccccagtc gaacgtctgc agggtgcgcc ccggcgtgat gttcacgccg
      481 acggagatgt ttttcggatc gagccccggg acggccgccc gcgccccgcg catcatctcg
      541 ccgatcagga acgacgcccc catcggatcg ttgcccggct cgccgccggt tcgggccagc
      601 tcggcgtttt ccatgcccgg cccgaccagg agcgccgtcc agagaaagaa ccacgccaca
      661 ttgccgagcg agccgcgcag gagcggcacc gactcgaaga gcaccgcgaa cgcggccacg
      721 agaaagagaa acggcaacgt cacgaggaga aacggcgcga cgagatcgac cggctggagc
      781 aggcgcgact cgccgcgcag gtactgcatg acacccgcca tcaccatgac ggcgaacacc
      841 agcacgccga gaagcgcggc gttgctcaac atcttcccca tcaggtagcc gaagcgggag
      901 agcggcgagg cgccgagaag ctgcccgacg cccgaccgtc gatcgcgctc gagcgaattc
      961 ttgacgagat agaaaccgat cagcgacagg aacaggctgg tcaggagcga caccgacacg
     1021 ccgacccagg cggagtcgta gaggccgcgc cagccatcca ggtcgaccgt ggcatagctg
     1081 gatgaattcg tcgggaggcc gagggatgcc gccgcgagga tggccaccag cgtgacgacg
     1141 aagccggagt tgcgtgtgcg ctcgagaaag tcgccgcgcg cgaaccggac aagcctcacg
     1201 ccgaccccgc ggacgcgggc tcgacgtgac agagataggc atcctcgagc gtgggagtgg
     1261 cagggagggc gccccccgcc gggcgctccc cgaccacgcg cacgagcacg tcaccgccgc
     1321 gtcggagcgc ccccgaaacg cgatggcgcc ggcgcacgtc cggcaactcg cccgcggcca
     1381 ccgaccattc ccacacctgc cccgacgccc gggccatcag ctcctcgggc gaggagaacg
     1441 ccacgagccg tccccggtgg atcagggcga tcgactctgc ggtggcttcc acgtcggaca
     1501 cgatgtgcgt cgacaacagc acgatccgcg atccggccag ctccgtcagc aggtgccgga
     1561 accggttgcg ctcctccgga tcgagcccca ccgtcggttc gtccacgata agcagcttcg
     1621 gatcgttgag gagcgcctgc gcgatcccca ggcgctgccg catgccgccg gagtagccgc
     1681 cgatggcccg cttcgccgcc tccgacaggt tgacgagcgc gagcagctcc tcgatccgct
     1741 ttttcgacac ggcggacgac aagccgcgcg ccgcggccag gtagtcgagg aactcgagcg
     1801 ccgtgaggtt gggatagatg ccgaagtcct ggggcaggta gccgaggacg cgacgcagcg
     1861 gatccggctt cgtcgcgatg tcctcgccgt tccagagcac gcgcccttcg gtggcccggc
     1921 ttaccgtggc caggatcttc atgagcgtgg attttcccgc cccgttcggc cccaggagcc
     1981 ccacgatccc cggcgcgagc gacagcgaga actcgcgcag ggcccagtga tcgcgtccgt
     2041 accgtttccc cagaccctcg aaatccagtt tcaaggcaac ctccggccaa agatcgcgta
     2101 tcacccgccg aatccgttcg cggcctgccg cactcgatcc gatcgctacg gcatccggcc
     2161 ggcgaatgtt gccgaaaggc tgttttggct acaatccctc gccggaactg atatccacag
     2221 acgttgaaac tgaccttcac tgactccgag gaggacactc ccgtgcccca gatgcatgaa
     2281 gtcgactaca aggtctacgg cgaagacctc cagttcgtcg aagtcgagct cgatccggaa
     2341 gaagcggtcg tcgccgaagc cggcggcatg atgtacatgg acgacggcat catgatggaa
     2401 accatcttcg gcgacggcag cgcgcagagc caggggaagg gattcctggg cagcctgatg
     2461 ggcgcgggga agcggctcct gaccggcgaa tcgctcttca tgacggtctt ctacaacaag
     2521 ggcgccggga agaagaaggt ggcgttcggc gcaccctacc ccggcaccat cctccccatg
     2581 cacctcggcc agatgggggg cgagctgatc gtgcagaagg actcgttcct ctgcgccgcc
     2641 aagggcgtat cggtgggcat ctccttccag aagaaactcg gcgccggcct cttcggtggc
     2701 gaaggattca tcatgcagcg cctgaccggc gacggcctcg cgttcatcca cgccggcggc
     2761 acgctctacg cgcgcacgct caatccgggt gaagtgctcc gcgtcgacac cggctgcatc
     2821 gtcggcttcc agccctcggt cgactacgac atccagatgg tcggcggcgt gaagtcggcc
     2881 cttttcggcg gcgaaggtct gtttttcgcc accctgcgcg gacccggcca gatctggctg
     2941 cagagcctgc ccctcaatcg catggcggcc cgcatcattg cggcgggcgg cagcgtgggt
     3001 ggacggcgcg aagaggggtc gatcctgggt gggttgggga acctgctgga cggggatagc
     3061 tgagggactc gccaaccgtg attggccata gtggactgtg ttccacacag aatcggaact
     3121 cggttcgaac agagttcccc ggaatggatg aaaaccgccg gaccgggccc tggtcaacga
     3181 cttggccggt tttacaagcg gtggcccggc ctttgcaatc cactatatgg tgatgaagcc
     3241 gcccggcgat gccgggcggc ttcgtacttg aggttctgac atcggcgggg cgaagccccg
     3301 ccgattccgg ctatttccgc ttcgtcgcca tcggcaccga cgtccgattg ccgttcgcgc
     3361 gcttggtgac ggcggacttc gtcgccccct tcgatttcga ggccggcgac gacgtcgcgc
     3421 gtggagcagc gcggcgggcc accgtcggga cgtcgaactc ctcctcatcg tccaggccat
     3481 ccaggtcgtc ctcgcgctgt ttcgactgtg cccgatcgct gatggccgcg cgggcgttgg
     3541 ccgcctgctc cgcgatctcg gtcagccgca tgtcggccgc ctcctcctca tccaacgtct
     3601 gctggaggag gtcggcggcg tcatcgaggc ccagttcgtg ggcgtgcgcg cgggcgcagc
     3661 cgtaggcggc catctcgtag tgctcgacgc gctgggcggc gccgatcaag ccggcgtcga
     3721 ggacggtgct gccgtcctcg ttttcctgaa ggagctcgct cccctcttcg agcaggcctt
     3781 ccatcccccg gcacttcttt cccctggggg agacgtcgag actctcgaag atcgtctcga
     3841 gtcgattgac atgttcccgg gtctgcccga ggtgatcctc gaaggccgcc ttgagctcgg
     3901 ggagctcggc gccgtcgatc aacctgggga gcgtggccag gatctggttt tccgcactgt
     3961 agatatcctt cagttcctcg acatagaggt cacgaagcgt cttgagtttc atggtgggac
     4021 cctcgttcac gggggattcc tggggcggcc atggaacgac tcccggaagg gatgcaggaa
     4081 tcgggcctcg ccgccggatg gcgctggttc gccggcgggg gcacaccggc ccgaaacgaa
     4141 tcaccccgct gacgaacttt tgtcccgccg ccgggcaatc aagtgactct gttacgcttt
     4201 tactttcgag cgcgaccggt tttcccgaca acctgatcgg cacaccgatg cggtgctcca
     4261 cggaggaatc atggcggagt ccatcaagac ctatctcatg ttcgacggca cggccgaggc
     4321 ggccatccgt ctttacgtat ctctcttcgc cggtgcggag atcacgaggc tggaattgcg
     4381 cggcgaatcg gagcctggaa aaccgggcac cgtgagtcgg gggaccttcc gactgctcgg
     4441 gcaggagtac atggccttcg acacgccgat gccgcacgcc ttcggcttca cgccggccat
     4501 ttccctgttc gtggagtgcg acagcgaaga ggagctcgac cggctgcacg cggcgttgtc
     4561 cgaggggggc accgagatga tgccccccgg ggactatggc t
//
LOCUS       DASVOD010000790         4909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_678240, whole genome shotgun sequence.
ACCESSION   DASVOD010000790 DASVOD010000000
VERSION     DASVOD010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 4909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4909
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678240"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..318
                     /locus_tag="VF720_16925"
     CDS             85..318
                     /locus_tag="VF720_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX7881097.1"
                     /translation="MNDETRRATIYLDAELHRALKHKAAETDRSLSELVNDAVRRSIA
                     EDAEDLAALRERAKEPTLKFEDFVRDLKRRGKL"
     gene            305..562
                     /locus_tag="VF720_16930"
     CDS             305..562
                     /locus_tag="VF720_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018146401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX7881098.1"
                     /translation="MASYSLTIRRSAAREIEALPKADRIRVIERIQRLADDPRPSGCE
                     KLSGEEKYRIRQGSYRILYEVTDSIVTVTVVKVGHRRDVYR"
     gene            complement(553..891)
                     /locus_tag="VF720_16935"
     CDS             complement(553..891)
                     /locus_tag="VF720_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881099.1"
                     /translation="MQREIDALPGADQRLDIQILGINSIGSEGGNSGMVAGRLLPWLQ
                     DTEAANVWVNWAITYRDVVIVDEENRVVDVYNLTSHSLADSANYATLRGKLLDAAVIE
                     GFATRAAGQR"
     gene            complement(928..1170)
                     /locus_tag="VF720_16940"
     CDS             complement(928..1170)
                     /locus_tag="VF720_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881100.1"
                     /translation="MSGFISPWRRAAAVLCFSAVLALGIAACGDDDETNNPPPEGVVP
                     DFALKDVNPASTTHNDDVSPRDYLGGVSAYYFGDAG"
     gene            complement(1257..3050)
                     /locus_tag="VF720_16945"
     CDS             complement(1257..3050)
                     /locus_tag="VF720_16945"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX7881101.1"
                     /translation="MTTDGDPATRRRLPRLTGLAGTVLGLAAAFAAWAKDGTIDARSV
                     RHDADGPDEVVWATAENPATRFLSVRFTCARADAISRAGLRIYRLDDQGLEGFRDHDA
                     SRYAGLDDLEVRALTVPLSDDPRVGPEGSRWRGYRIWLDDQDRLFWSGRDSVSTKESV
                     VPRFDRLFPPARNGQGISDHIITIPGWATDAVAIRIDVATFRNGDPSNDPKDGRPGGD
                     LAGLAAAIPSLDSLGVTLVLLSSLVTTCPIDPTAPIDLMSIDPRYGNLELFEKVVQQA
                     HSREMRVVATLPLAAISRSNPWVSDALYFKEESAFHTFFDFPRTDDGAIANAADTTGT
                     VPWNADNPEVVDELLKPVQLWARTRLDGWYLPDLDSQPPSFWQSLTAAMKALDPKSWI
                     IGQGTGPLDRWMTGDLVDAIDTPAYEAAVMPFATGKERDAMTLCAALDKRRVETPEPF
                     TRTSVNPLGAAPDDAAGRLATFLQLTSEGVPLLDASSPLLSGSRRWVRELIEIRKEHP
                     SLRRGSVEPIYVQGGVACVLRRTSEEMFLVVVNQEPTPLTTRLPIRGSFGQVSQKDAV
                     DLLSGTRHAIRSGELFLKDVPAGGMALVRLR"
     gene            complement(3055..3459)
                     /locus_tag="VF720_16950"
     CDS             complement(3055..3459)
                     /locus_tag="VF720_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX7881102.1"
                     /translation="MVTRSADPLGEKMKHHEGFLNLVTDAKSRVKECNAGDVLTRLTQ
                     GADSVVIDVREESEWEAGHIRGAVHLGKGIIERDIARVVPDNDREIILYCGGGFRSAL
                     AADNLQKMGYTNVLSMAGGWRAWKENDYPSET"
     gene            3546..4295
                     /locus_tag="VF720_16955"
     CDS             3546..4295
                     /locus_tag="VF720_16955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX7881103.1"
                     /translation="MKESKQARDARSLREMFEMIGERFLDLPFTKGTAQEVEFLVEML
                     DLTPKDRLLDLACGPGRHAVALGKQGIRVTGVDLSDRLLALAAQAAELEGVTTVEFVK
                     GDAREMPYNEEFDAAICLCEGAFGLLETDIENERVLNAIYRALKPGGRMALTCLSLYH
                     LMRKGQDLTAFDPQTNMYEREEMIQVEGGSEELFRFHERYYDFPGLKILLERVGFRHI
                     LGFGCRAGEYSRRAITLDDVEILVYAQKPVR"
     gene            complement(4435..>4909)
                     /locus_tag="VF720_16960"
     CDS             complement(4435..>4909)
                     /locus_tag="VF720_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881104.1"
                     /translation="LLTVGLFLPAAVFAQMSDKVAVIRIKAIEGLVADNAPVQVSRDQ
                     AFLAGSSLRLSKAAVGSLEGVTAIVLIDRRTDSMSGSDLKKHHQEEVLTVTGDTFTVK
                     GVDGQNVTYALDHLSDGSVDLLGPADRIIARGQGQFKVWKVEGTSMMSRDKISLY"
BASE COUNT          847 a         1560 c         1679 g          823 t
ORIGIN      
        1 aaacccgttg gcggcaagcc ctttccgcgc ggggcgttgt aaaacgtctt gacgctaaaa
       61 cgtcaaaacg gcacactcga tgggatgaat gacgagacac ggcgcgccac gatctacctg
      121 gatgcggagc ttcatcgcgc cctgaagcac aaggcggcgg aaaccgatcg gagcctgtcg
      181 gagctggtga acgatgccgt gcgtcggtcg atcgcggagg atgccgaaga cctggccgcc
      241 ctgcgggagc gggccaaaga gccgacgctc aagttcgagg acttcgtccg ggacctgaag
      301 cggcgtggca agctatagcc tcacgatccg tcgctcggcc gcccgggaaa tcgaagcgct
      361 ccccaaggcc gaccgaattc gcgtcatcga acggatccag cgcctggccg acgatccgcg
      421 cccgtcggga tgcgagaagc tctccggtga agaaaagtac aggatccggc agggaagcta
      481 ccggatcctg tatgaagtga ccgattcgat cgtgaccgtg accgtcgtca aggtcggcca
      541 tcgtcgcgac gtctatcgct gacccgctgc cctcgtagca aacccctcga tcaccgccgc
      601 atccagcagc tttccgcgca gcgtggcgta attggccgag tccgcaaggg aatgcgacgt
      661 gaggttgtag acatccacca cccggttctc ctcgtcgacg atcacgacgt cgcggtaggt
      721 aatggcccag ttcacccaca cattggccgc ttccgtgtcc tggagccagg ggagaaggcg
      781 accggccacc atgccggagt tgcccccttc ggagccgatg ctgttgatgc cgaggatctg
      841 gatgtcgagg cgctggtcgg cgccggggag cgcgtcgatc tcgcgctgca tctggtcgag
      901 gtagccaaac tggctacggc agtagcctca gccggcgtcg ccgaagtagt acgccgacac
      961 gcctcccagg tagtcgcgcg gggagacgtc atcgttgtgc gtggtcgagg cggggttgac
     1021 atccttcagc gcgaagtcgg gcaccacgcc ttccggtggg ggattgttcg tttcgtcatc
     1081 gtcaccgcac gcggcaatgc caagggccag cacggcggag aagcacagca ccgcggcggc
     1141 gcgccgccag ggggagatga aaccactcat ttcgcattcc tcgttgttcg ttccggtcga
     1201 tcgtgcgcaa ttctcgatgg ccgcccgtgg cggcgatgag cccgggaagt atacgcctat
     1261 cgcaggcgga ccagcgccat cccgccggcc ggcacgtcct tcaggaagag ctcgccgctc
     1321 cggatggcat ggcgggttcc cgacaggagg tccaccgcgt ccttctggct cacctgcccg
     1381 aaactgccgc ggatcgggag cctggtcgtg agcggggtcg gctcctggtt caccaccacc
     1441 aggaacatct cctcggaggt gcggcggagg acgcaggcca cgccaccctg cacatagatc
     1501 ggttccacgc ttccccggcg gagcgacgga tgctccttcc ggatctcgat cagttcgcgc
     1561 acccaccgcc gcgatccgga caggaggggc gatgaggcat cgagaagcgg aacgccttcg
     1621 ctcgtgagtt gcaggaatgt ggccaggcgg ccggccgcgt cgtcgggtgc cgccccgagg
     1681 gggtttacgg acgtgcgcgt gaacggttcg ggggtctcca cgcgacgctt gtcgagcgcg
     1741 gcgcagagcg tcatcgcgtc acgttccttt ccggtggcga agggcatgac ggcggcttcg
     1801 taagcgggcg tgtcgatggc gtcgaccagg tcgccggtca tccatcgatc gaggggcccc
     1861 gtgccctggc cgatgatcca gcttttcggg tcgagcgcct tcatcgccgc ggtcagcgac
     1921 tgccagaagg acggtggttg cgaatcgagg tcggggagat accacccgtc gaggcgcgtc
     1981 cgtgcccaga gctgcaccgg cttcagcagt tcatcgacga cttccgggtt gtcggcattc
     2041 catggcaccg tgccggtggt gtccgccgcg ttggcgatgg ccccgtcgtc ggtgcgcgga
     2101 aagtcgaaga aggtgtggaa ggccgactcc tccttgaaat aaagcgcatc cgacacccag
     2161 ggattcgagc gactgatcgc ggccagcgga agcgtggcca cgacccgcat ctcgcgactg
     2221 tgggcctgct gcaccacctt ctcgaagagc tcgaggtttc catagcgggg atcgatcgac
     2281 atgagatcga tcggggcggt cggatcgatc gggcaggtgg tgacgaggga cgagaggagg
     2341 accaatgtca cgccgaggct gtcgagcgag gggatcgccg cggcgaggcc ggccagatcg
     2401 cctccggggc gaccatcttt cggatcgttg gagggatcgc cgttccggaa ggtggccacg
     2461 tcgatccgga tcgccaccgc gtcggtggcc cagccgggga tggtgatgat gtggtcggaa
     2521 attccctggc cattccgggc aggcggaaag aggcggtcga agcggggaac gaccgactcc
     2581 ttcgtgctca ccgaatcgcg ccccgaccag aagaggcgat cctggtcgtc gagccagatc
     2641 cggtagccgc gccagcggct cccttccggt cccacgcgcg gatcgtcgga gaggggcacc
     2701 gtgagggcgc ggacctcgag atcgtcgagt cccgcatagc gggacgcgtc gtggtcgcgg
     2761 aatccctcga gtccctggtc gtccaggcgg tagatgcgga ggcccgcccg gctgatcgcg
     2821 tcggcgcggg cgcaggtgaa gcggacggaa aggaagcggg tggcgggatt ttccgcggtg
     2881 gcccacacga cttcgtcggg tccgtcggcg tcgtggcgga cggagcgggc gtcgatcgtc
     2941 ccgtcttttg cccacgccgc gaatgcggca gcgaggccga ggacggtgcc ggcaagcccg
     3001 gtgagcctgg ggagacgccg gcgcgtggcc ggatccccgt cggttgtcat gccatcaagt
     3061 ctcggagggg tagtcgtttt ccttccaggc ccgccagcca ccggccatcg agaggacgtt
     3121 ggtgtatccc atcttctgga ggttgtcggc cgcgagggcc gatcggaagc cccccccgca
     3181 gtagaggatg atctcgcggt cgttgtccgg gaccaccctg gcaatgtcac gctcgatgat
     3241 tcccttcccg aggtggacgg ccccccggat gtggccggcc tcccactcgc tctcctcgcg
     3301 cacgtcgatg accacgctat cggcaccctg ggtgagacgc gtcaggacgt ccccggcgtt
     3361 gcactctttc acgcgcgatt ttgcgtcggt gaccaggttg agaaatccct cgtgatgctt
     3421 cattttttcc cccagtggat cggccgatcg tgtcaccatg gccgtgaccg acctgcgaac
     3481 cgtagaggac aaccgacgcc gggacaaggg cgccggaagc gcgcaacgac cgccccggag
     3541 gccagatgaa ggaatcgaaa caggctcgcg acgcgcgatc gctccgcgag atgttcgaaa
     3601 tgatcggtga gcgcttcctc gacctgccgt tcaccaaggg caccgcccag gaggtggaat
     3661 tcctggtcga gatgctcgac ttgacgccga aagaccggct cctcgacctg gcctgcgggc
     3721 cggggcggca cgcggtggcg ctcggcaagc aggggatccg ggtgacgggc gtcgaccttt
     3781 ccgatcgcct cctggccctg gcggcccagg cggccgaact ggagggcgtc accaccgtcg
     3841 agttcgtgaa gggcgatgcc cgcgaaatgc cgtacaacga ggagttcgac gccgccatct
     3901 gcctgtgcga gggggccttc gggctcctcg aaaccgacat cgagaacgag cgcgtcctga
     3961 acgccatcta ccgcgcgttg aagcccggcg gccgcatggc gctcacctgc ctctctctct
     4021 atcacctcat gcggaagggg caggacctca cggccttcga tccgcagacc aacatgtacg
     4081 agcgggaaga aatgatccag gtggaaggag ggagcgagga gttgttccgc ttccacgagc
     4141 gttactacga cttccccggg ctcaagatcc tgctcgagcg ggtcggcttc cgccacatcc
     4201 tcgggttcgg atgccgggcc ggcgaatact cgcgacgggc catcacgctc gacgacgtgg
     4261 aaattctggt ctacgcgcag aagccggtgc ggtagcccgc tgcgggtcgc gcccgccgtc
     4321 gatgtgccga aaagacgaag ccggagatac tggtcgtatc tccggctcgt ttttcccccc
     4381 agcgtttgga agacgccggc ccggcatgcg gcggcgagcg ccgcggtacc gtacctagta
     4441 cagcgagatc ttgtcccgcg acatcatcga ggtgccttcc accttccaca ccttgaactg
     4501 cccctgcccg cgggcgatga tccggtcggc ggggccgagc agatcgaccg aaccgtcgct
     4561 gaggtggtcg agggcgtagg tgacattctg gccgtcgacg cccttcacgg tgaaggtgtc
     4621 gccggtgacg gtgaggactt cttcctggtg gtgcttcttc aggtcgctgc cgctcatcga
     4681 gtcggtccgg cggtcgatga ggacgatggc ggtgacgcct tcgagcgaac cgacagcggc
     4741 cttcgagaga cgaagggacg agccggcaag gaaagcctgg tcgcgactca cctgcacggg
     4801 cgcgttgtcg gccacgaggc cctcgatcgc cttgatccgg atcacggcaa ccttgtcgga
     4861 catctgggcg aaaaccgcgg ccggcaggaa aagtccgacg gtcagcagc
//
LOCUS       DASVOD010000791         5279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Eiseniibacteriota bacterium isolate
            SMAG_U3144 k141_678247, whole genome shotgun sequence.
ACCESSION   DASVOD010000791 DASVOD010000000
VERSION     DASVOD010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959772
            Sequence Read Archive: SRR10695394
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Eiseniibacteriota bacterium (soil metagenome)
  ORGANISM  Candidatus Eiseniibacteriota bacterium
            Bacteria; Candidatus Eiseniibacteriota.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 02:56:53
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,397
            CDSs (total)                      :: 3,363
            Genes (coding)                    :: 3,353
            CDSs (with protein)               :: 3,353
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 1 of 10
            Pseudo Genes (incomplete)         :: 9 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Candidatus Eiseniibacteriota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678247"
                     /isolate="SMAG_U3144"
                     /isolation_source="soil"
                     /db_xref="taxon:2212470"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="23.2097 N 104.1342 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF720_16965"
     CDS             <1..548
                     /locus_tag="VF720_16965"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX7881105.1"
                     /translation="DASTRHELADVSDEWLTMQRTREMRFSLGRFDPDYLAESAMVIS
                     RDGAGRITAFANANRASAASLIGLDLMRRRRQVEPGTMDTLMVALIEWARGEGLAEVS
                     LGLSPLAGVGGGPDAPVAERGLGFVYRRLDHYYNFRGLHDFKSKFQPRWYERFLAHPG
                     GASLPGVATAMLSATLADRVW"
     gene            complement(573..1307)
                     /locus_tag="VF720_16970"
     CDS             complement(573..1307)
                     /locus_tag="VF720_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881106.1"
                     /translation="MRRPNTRLLASFLPALIVFLPIVSLTQRTGPAWGADPGPGPDRT
                     DKSSRPGTSDPTTGDVPTFSVSGDVFRELSDEAEHHFKQAREAMTRGEPARAAADIRK
                     AASYLRLEASRGHEADAGRLRAEASTIQALANRTTTIPDSELVSAFAHADLVLAHHHF
                     DRLERDWRDHDIDDTAADIVATRHHLLAARGWHGGDVPASIVAGYDQAGALAARMGQP
                     IGWSAAEVDSVKVFLAGELGRQQGGS"
     gene            1504..2220
                     /locus_tag="VF720_16975"
     CDS             1504..2220
                     /locus_tag="VF720_16975"
                     /inference="COORDINATES: protein motif:HMM:NF024957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEX7881107.1"
                     /translation="MLFRRVTLAGLIGGLILLVAPPAHAQVLIGYLLGEKLSTPVFNM
                     GFEVGANFSKLDGFEEGGRRNGPVFGLFADWRFSEHVHLGGAILPIAGRGAKDLDPAQ
                     TGDAAIDGQTAESRMERSLGYVEIPLILKWSPHRQEGIRIGAGPSFGFVTGADDRYAC
                     RTPDGAPYTIERDIKGLIPGFDFGLSAEAEYRLEFLSIAVRYTQGLTDMRVEGTDEAV
                     HTRVLTGTGRIYLGKKPAEH"
     gene            complement(2352..4862)
                     /locus_tag="VF720_16980"
     CDS             complement(2352..4862)
                     /locus_tag="VF720_16980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX7881108.1"
                     /translation="MRALPILVQLALAVAVLTGYRVESASFRTALLALLVAYPVHVML
                     PARARLPFFAMLGVAALVFVAGPVSAAWILALGGSLIALAHLPIPFRARLGLIVMAAV
                     ALGVLRATPPTTLPRPWSEVVWPILASMFMFRFLVYLYDLSHKSAPFSFWRAVAYFFM
                     VPNVWFPLFPIVDYQAFNRAAPEAGRFRNHQIGIDWVVRGAVQLILYRVVYQHLLVDP
                     VTIAGLGDLVRALVTTFLLYLRISGSFHLIVGILRLFDFALPETHHLYFLASGFSDFW
                     RRINIYWKDFVMKLVFYPAFFRLRKTNPAGAMVPATLLAFAATWALHSWQWFWMCGSF
                     LVSLTDTLFWAILAVLVTIDAVRESKPGARATRARGWNADLRRALATIGTFSFLTVLW
                     SFWTVGSWGEFTAMFGRVRSVSAADVGLVAAILAVIGTWSVIDNRTRRLPARAGTTSS
                     GWLTRETWLSRDVMTNAAVALILVALAMRPVQARLPEPAGRFFAELESPRLNDQDLAL
                     LQRGYYEDLTQVHRFSPDLAIVYGERPPDYFIPWRQNLRDETTDYFQEVLKPNAAYTY
                     MDVRYTTNRWGMRDRDYARERTPGVPRVALVGSSYTLGWGVADDETYESIAEKRLATA
                     GTPVEILNFAVHAYDPVRNDLVLEQRVMPFHPDVAIVFCNTNERKTLVTNLAHAIEQG
                     IPLPYPFLEEARAAAGVNRTTERAVMELRLADVADAAIEAAWTRFVTDCRAAGIVPIW
                     IFMPETIARVPGQSTEQVPRFYEMARRAGFDVVTDLAPAFTGVDGETLKLFPWDYHPN
                     VRGHELLAVEFENVLRREVLPRIGKSGVGGAAGGPSGR"
     gene            5040..>5279
                     /locus_tag="VF720_16985"
     CDS             5040..>5279
                     /locus_tag="VF720_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX7881109.1"
                     /translation="MHTSPNVKVVTGRPQSNWGDAVAVPVFKGRGAVRGTLFTALDKQ
                     LKGALTDALALSDFNGGEDVKTVYKRSKALPPRVLL"
BASE COUNT          909 a         1661 c         1892 g          817 t
ORIGIN      
        1 tcgacgcctc gacccgccac gagctggccg atgtgagtga cgagtggctc accatgcaga
       61 ggacccgtga gatgcggttc tcactcgggc gcttcgatcc tgattacctg gccgagtccg
      121 cgatggtcat ctcccgcgac ggcgccggcc ggatcaccgc cttcgccaac gccaacaggg
      181 catcggcggc ctcgctcatc ggcctcgacc tgatgcgccg ccgccggcag gtggagccgg
      241 gaacgatgga caccctgatg gtagcgttga tcgaatgggc ccgcggtgag ggcctggccg
      301 aggtcagtct cgggctctca ccccttgccg gagtcggggg cggtcccgac gcccccgtgg
      361 ccgagcgggg gctcgggttt gtgtaccggc ggctggatca ctactacaac ttccgcggac
      421 tgcatgactt caagtcgaaa ttccagccgc gatggtatga gcggttcctg gcgcatcccg
      481 gtggtgcgag cttgccgggg gtggcgacgg cgatgttgtc ggcgacgttg gcggatcggg
      541 tgtggtaggg gggccggact gagtctggct gactaactcc cgccctgttg acgtcccaat
      601 tctccggcga ggaagacctt cactgaatcg acctccgccg cgctccaacc gatcggctgg
      661 cccatccgcg ccgccagggc gccagcctgg tcgtagcccg ccacgatgct ggcggggaca
      721 tcgccaccgt gccagccacg ggcggccagc aggtggtggc gggtagcgac gatgtcggcg
      781 gcggtgtcgt cgatgtcgtg gtcgcgccag tcgcgttcca gtcggtcgaa gtgatggtgg
      841 gccaggacga ggtcggcgtg tgcgaaggct gagaccagtt cactgtccgg gatggtcgtg
      901 gtgcgattgg cgagggcctg gatcgtgctg gcttcggccc ggaggcggcc ggcatcggcc
      961 tcatgtccac gcgacgcttc gagacgcagg tacgaagcgg ctttccggat atcggcggcg
     1021 gcgcgcgccg gttcgccgcg cgtcatggcc tcacgggcct gcttgaagtg gtgctcggct
     1081 tcgtcggaca gctcgcggaa gacgtcgccg gagacactga acgtcgggac gtcgccggtg
     1141 gtggggtcac tggtaccggg gcggcttgac ttgtcggtgc ggtccgggcc gggtccgggg
     1201 tcggcgcccc aggcgggtcc ggtgcgctgc gtgagggaga cgatggggag gaagacgatc
     1261 agggcgggaa ggaagcttgc gagtaaccgg gtgttcgggc gacgcatgac gttcaacctc
     1321 gggagccggg cgccgctcgg ccccggtcat tccgggcgag gggtgggaag ccggcccacc
     1381 cggcctcgta caccgggagg aggttggggt tcgcgaactg tgggggacac aggtctcact
     1441 tcgtccccgc tcgccggacg ggattcgtgg acgtcatcac ccggtcgagt gtaggctgcc
     1501 cccatgctat tccgccgcgt cacgctggct ggcctcatag gcggcctgat ccttctcgta
     1561 gccccgcccg cacacgcaca ggtcctgatc ggctacctcc tgggcgagaa gctgtcgaca
     1621 ccggtcttca acatgggatt cgaggtcggc gccaatttct cgaagctcga cggtttcgag
     1681 gagggggggc ggcgcaatgg gccggtgttc ggacttttcg cggattggcg gttctccgag
     1741 cacgtccacc tgggcggggc catcctgccg atcgcggggc gcggggccaa ggacctggat
     1801 cccgcccaga ccggggatgc cgcgatcgat gggcagacag ccgaaagccg catggaacgc
     1861 agcctcggct atgtcgaaat cccgctcatt ctcaagtggt caccgcatcg gcaggagggg
     1921 atccggattg gagccggccc ctcgttcggg ttcgtgaccg gtgccgatga ccgctacgcc
     1981 tgccgaacgc cggacggagc gccctacacg atcgagcgcg acatcaaggg cctcatcccc
     2041 ggcttcgatt tcggcctctc cgccgaagcc gaataccgcc tcgagttcct gtcgatcgcg
     2101 gtgcggtaca cgcagggcct caccgacatg cgcgtcgagg gtaccgacga ggccgtgcac
     2161 acgcgcgtcc tgactggaac cggccgaatc tacctgggga agaagccggc cgagcattga
     2221 tggactgccg gcccggagcc ggcaactatg ttcaggcagg gcctggacgc cgggttcgac
     2281 cgccgatcaa ctttgttcag gcagagtccg gacgccgggt ccgaccgccg atcaactatg
     2341 ttcagaccga gtcaccgccc tgacggaccc cccgccgcac ccccgacgcc cgactttccg
     2401 atccgtggca acacctcgcg ccgcaacacg ttctcgaatt cgacggcgag gagttcgtgc
     2461 ccgcggacgt tggggtggta gtcccacggg aagagcttca gcgtttcacc gtcgacgccg
     2521 gtgaaggcgg gggccaggtc ggtcacgaca tcgaagccgg cgcggcgggc catctcgtag
     2581 aaacggggca cctgctcggt tgactggccg gggacccggg cgatcgtctc gggcatgaag
     2641 atccagatgg ggacgatgcc ggcggcacgg cagtccgtga cgaagcgggt ccaggcggct
     2701 tcgatggcgg cgtcggcgac gtcggccagc ctgagttcca tgacggcgcg ctccgtggtc
     2761 cgattcacgc cggcggcagc gcgagcttcc tcgaggaagg ggtaggggag tggaattccc
     2821 tgctcgatgg catgcgccag gttcgtgacc agcgtcttgc gctcgttggt gttgcagaag
     2881 acgatggcca cgtcggggtg gaacggcatc acgcgctgct ccagcacaag gtcattgcgc
     2941 accggatcgt aggcatgcac cgcgaagttc aggatctcga ccggcgtgcc tgcggtggcc
     3001 aggcgcttct cggcgatcga ctcgtacgtt tcgtcgtcgg ccacgcccca gccgagcgtg
     3061 tagctcgatc cgaccagcgc cacgcggggg acgccgggtg tgcgttccct ggcgtagtcg
     3121 cggtcgcgca tcccccagcg gttggtcgtg tacctgacat ccatataggt atacgcggcg
     3181 ttcggcttca gcacttcctg gaagtaatcg gtcgtctcgt cacgcaggtt ctggcgccac
     3241 gggatgaagt aatcgggcgg gcgctcgccg tacacgatgg cgaggtcggg gctgaagcgg
     3301 tgcacctggg tcaggtcttc gtaatagccg cgctggagga gcgccaggtc ctggtcattg
     3361 aggcgagggc tctcgagctc ggcgaagaag cggccggccg gttcggggag ccgcgcctgc
     3421 accgggcgca tcgccagtgc cacgaggatc agggccacgg cggcgttggt catcacgtca
     3481 cggctcagcc aggtctcccg cgtcagccag ccggacgatg tcgtgccggc gcgagccggc
     3541 aaccgcctgg tgcgattatc gatgacgctc caggtcccga tcacggccag gatcgcggca
     3601 acaagaccga catcggccgc cgacacggag cgcactcggc cgaacatcgc ggtgaactcg
     3661 ccccacgagc cgaccgtcca gaacgaccag aggactgtca ggaaactgaa cgtgccgatc
     3721 gtggccagcg cgcggcggag atccgcgttc cagccgcgcg cgcgggtggc ccgggcgccg
     3781 ggcttcgatt cgcgcacggc gtcgatcgtt accagtaccg ccaggatcgc ccagaagagc
     3841 gtgtcggtca gtgacacgag gaacgagccg cacatccaga accactgcca tgagtggagg
     3901 gcccaggtgg cggcgaaggc caggagcgtg gcgggcacca tcgccccggc ggggttcgtc
     3961 ttgcggagcc tgaaaaaggc cgggtagaag acgagcttca tcacgaagtc tttccagtag
     4021 atgttgatgc gccgccagaa gtcggagaaa cccgacgcca ggaagtagag gtggtgggtc
     4081 tcggggagcg cgaagtcgaa caatctcagg atcccgacga tcaggtgaaa actcccggag
     4141 atgcggagat agaggaggaa tgtcgtgacc agcgcgcgca cgaggtcgcc cagtccggcg
     4201 atcgtgacgg gatcgaccag caggtgctga tagacaacgc gatacaggat gagctgcacg
     4261 gcgcctcgca cgacccagtc gatgccgatc tggtgattcc gaaatcgccc ggcttccggc
     4321 gcggcgcgat tgaacgcctg gtagtccacg atcgggaaga gggggaacca gacgttcggc
     4381 accatgaaga agtaggccac cgcacgccag aagctgaatg gcgcgctctt gtgggacagg
     4441 tcgtacaggt acaccaggaa ccggaacatg aacatggaag ccaggatcgg ccacaccact
     4501 tccgaccagg ggcgggggag ggtggtcggc ggcgtggccc ggaggacgcc gagcgccacg
     4561 gccgccatga cgatcagccc gagccgggcc cggaacggaa tggggagatg cgcgagggcg
     4621 atgaggcttc cccccagcgc caggatccag gcggcactga ccggaccggc cacgaagacc
     4681 agggcggcga cgccaagcat ggcgaaaaac gggagccgcg cgcgggccgg caacatcaca
     4741 tggacgggat aggcgacgag gagcgcgagg agggcggtac gaaaggaggc ggattcgacg
     4801 cggtaaccgg tcaggaccgc cacggccagc gcgagctgga ccaggatggg aagcgcgcgc
     4861 attcggcggt cgatcataac acgcgggggg gacacgggcc cggacagcca cgatccaccc
     4921 gcactggccc ctcgtcaaaa ccgctgatat agtgccttcc cttcgaagcc gcctgtcggc
     4981 gcgtcgagtg gccacgcaca gtcaaccggc cgcggccggt cgtccttgac ggagacctca
     5041 tgcacacgtc gccgaatgtg aaagtcgtga cgggccgccc gcagtcgaac tggggcgatg
     5101 ccgtggctgt accggtcttc aaggggcgcg gcgcggttcg cgggaccctt ttcaccgcgc
     5161 tcgacaagca gttgaagggc gcgctcaccg acgcgctggc cctctccgac ttcaacggcg
     5221 gtgaagacgt gaagacggtg tacaagcgaa gcaaggcgct gccgccccgc gtgctcctg
//
